LOCUS       DAWEJW010000001       100869 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_100_length_100869_cov_55.918722, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000001 DAWEJW010000000
VERSION     DAWEJW010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 100869)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 100869)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..100869
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100_length_100869_cov_55.918722"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10439..10538
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15503..15602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    42038..42137
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    43424..43523
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    57466..57565
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        23836 a        27303 c        19649 g        29581 t
ORIGIN      
        1 aagacaaccg tctcgacatt tgcggtccag gggaactggt ccacggccac ggccttgtcc
       61 acgcggtaac cgttctccag aaacacttcc agatcccgca caaggctggt gggtttacag
      121 gagatataaa cgatatgatc cacgccgtaa gcaatgatct tcggcagtgc cttggggtgg
      181 atgccgtctc tggggggatc caggatgatc atatccggct tctcttccac ttcgtccaga
      241 accttcagta catctccggc gatgaattcg cagttatcca gtccgttggc agcggcgttt
      301 ttcttcgctg cctccacggc ttcctccacg atctccacac cgatgacttt cccggcggcc
      361 ggtgccatga gctgtgcgat ggtgccggtg ccgctgtaga ggtcgaacac agtcttgtcg
      421 ctgccgccca ggtcgcccac ataatctctc gccgtctggt acagtacctc cgcactgtaa
      481 gagttggtct ggaaaaagga gaatgtggag atcttgaatt tcaaccccag cagttcctca
      541 taaaaatact cctgtccgta taacagctcc gtacgatcac tctgcaccac atctgcaatg
      601 gaatcattgg taatatgtaa gattcctgcg aattttccgg ctagcttcct atcctgttcc
      661 agggaaagca gcagatcctt aaatcctgtg atcagcgcat ccacatccaa gcttccttcc
      721 accgccgtct ctccctgcca gggatcctgg gaggtcgtca ccagcgccac cagaatctct
      781 ccggtgcggg atgccttacg taccagcagg tgacgtaagt acccctcatg gctcattctg
      841 tggaaaaagc ttaccttctc ccgggcaaag taatctctca ctgtctgtaa gatcaggcgg
      901 taatccgcat ccacgatctc acagtccgct acggtcacta tatcgtagaa gctgcctctc
      961 ttatgcatgc ccagcgccag cggtccgtcc ttgtactcgt ctccgaagga aaattccatc
     1021 ttattacggt attcataagc cttcgggctg cccttgatgc cctcaaaagc ccaggcttcc
     1081 tcctgcttgt ttaacacact gtccagcagg cgttttacct gttcctcctt gactttcagc
     1141 tgttcttcgt aaggaagaga cagataggta catccgccgc acaacccaaa aagggaacac
     1201 gtccggccgg tttccagagg tgacttttcc gtcacctcca aaagccttcc ttccgccttc
     1261 cctttgcgca ctttattcac cgagaacttg actttctgtc cgggcaggct gttcttgact
     1321 acagcagtct catctcccac acagacgatc cctttgttgg gaaaatcgac ccttacaaca
     1381 gatccttcat atacctgtcc ttttttcatg ggttagttct ctccttcatc ctcgaagaag
     1441 tcgtcatcat cctcttccac gtcatctgct tcatcttcga agtcatcctc gaagtcttcc
     1501 agataatcct gtgcaaagaa gaaacggtat acagcataag ccactgctgc aacagctact
     1561 acggcgccga tgatggccag tacccataac aggatattgc gttccttctt ctcttctact
     1621 tccttgccca acagcttgtt aagcttcttc agttcgctga ctttcagctc atttaatttg
     1681 tcaagatcta ccagattttc aattctgttc atattattta cctgcctttc ctgcatctat
     1741 tcgtatttat ggatgcaaag cattttgttt tcattccttt atatgatacc atattttcat
     1801 caaaaatact atagaaaaac catatttgag gaaatacatt tcttattctt atattcttat
     1861 aatttcttac aactgcttta gttttgcaaa agacgcatac ataatcttct gccctgcctg
     1921 gtcgaattcc acggtcacct tatagtctct gggttctctc acgatattta gtaccgtgcc
     1981 ctcgccgtat ttgatatggc gcactctgtc acctacctcg tagtccagtt tctccggtgc
     2041 cttgaactcc gtgcccttct gcagtccggc caactggttc agtccgctga tgcccttgga
     2101 aatataaggt ttatccgcct ttgccgtaac acggggtttt aaggtcgcct tgggtcttgg
     2161 atcaaagccg gtattaccga agccgccaaa accactatcc gagccggatc cagaccgggg
     2221 agatgcaatt gcctccagtc ccatggcacg aagagctgct tcattggctc ttctcgcctg
     2281 aaaatcttcc agatcatcgt cccggtatct tctgctgggc ggcagggtat tatccaataa
     2341 ttccttcgga atctccctca caaaacggct caccggattg tactgggtct ccccacgcag
     2401 cattctgctc tttgcacatg tcagcgtcag atcctccttg gctctggtga ttcccacata
     2461 agccagtctt ctctcttcct cgatctccaa cgggtcatcg gaagcaatgg tcataaagct
     2521 ggggaataat ccatcttcca tgccggcgag atacaccacc gggaattcca ggcctttggc
     2581 actgtgcagc gtcatgagca gcacccggtt gtcaccgttc tccacattgt caatgtctgc
     2641 caccagcatg atttcttcca gaaattccga cagagaaggc tcgtcgtgag tctcctcata
     2701 tgcggccgcc ttggtgatca attcatctac gttccccaga cgatcctcag cggattcctc
     2761 atcctgatcc cgcagatatt ctgcataatc gatccgttcc agcagagatt tgatcagatc
     2821 cggcaatgta tacgaagaca atccgctgcg aagtacctcg atcagattca caaatccacg
     2881 gatcttggtc tccgctcttc ccagtccctt gatctgatct gcttcacaca atgcatcaaa
     2941 taaagtaata tccttcatct gtgcatagtc tgctaccttt tccaaggatg ccgcaccgat
     3001 gcttcttttc ggcacattaa taattctgcg cagagccact tcatccctgc cgttatcaat
     3061 agttttcaga tatgccagaa tatcgcggat ctcctgtctg gcatagaagt tcactccacc
     3121 taccacatga taaggaatgc cctccactac catacgttct tccagaagtc gcgactgggc
     3181 attggtacgg tacaacaccg cacagtccgc ataggcaatg ccgtcatttt tcaccttgtc
     3241 agcaatatcg tccgcaatgt attccgcctc ttcataggca ttgtcaaact gcctgaaatg
     3301 tacccggctg cctgatcctt tttccgtcca gagggctttt tccttacggc ctctgttgtt
     3361 acggatcacc gcgttggccg catccaggat gttctgggtg gatcggtagt tctgttccag
     3421 cttaatcacc tttgcatcgg gatatacctt ctcgtagtcc agaatattac ggatattggc
     3481 accgcggaat ttgtagatgg actggtcatc atcacctacc acacacagat tgcgataacc
     3541 gtccgccaac agacggatca gttcgaattg cgctgtattg gtatcctggt actcatccac
     3601 catgatatat ttgaaacgtt cctgataatt ataaagtact tccgggcagc ttttgaacag
     3661 ttccacagtc tttacaataa tatcatcaaa atccagtgca ttactctttt tcaaggtctc
     3721 ctgatactct ctgtagacct tggcgatcac ggatttcttg taatctccca cggattttac
     3781 ttcaaattcc cgcacatcca ccagctcgtc cttggcggag gaaatttccg ataacagcgt
     3841 ccgctctttg tacatcttcg tgtcgatgtt caggcgcttg cagacctgct ttatcacaga
     3901 tttctggtca tccgtatcat aaatggtaaa attcgtatca tagcccaacc ggtcaatatg
     3961 tcttctgagg atcctcatgc aagtggcatg gaatgttgct acccatacct gatccgcacc
     4021 gaaacctgcg atctgatcca cacgctcccg catctcctgg gctgctttgt tggtaaaagt
     4081 gatggccaga atgttccatg gatttactcc catctcatcg atcagataag ccactctgtg
     4141 ggtcaggaca cgggtcttgc cgcttcctgc gcccgccaac agaaggagcg gtccttccgt
     4201 atgaagcacc gcttcttttt gttctttatt taaggtatca taaatactca tgaattacgc
     4261 attatccctt tcattaaatt cctgcaaaaa ctttcggaaa tcatcgattg gcataggctt
     4321 tgcataaaaa taaccctgta cgatgtcaca gtccgccaga cgcagcatgt ctaactgttc
     4381 cttcgtctcc accccctctg ccaatacctt gaagccgaga tgcttcaaca ttttagtcag
     4441 atatttgata attgtcaatg ccgtttcttc ccgttcgctg ttcatggtgt catccaggaa
     4501 ctgcttgtcc agcttcaccg tatccaccgc taccttggac aacagattca gggatgaata
     4561 accgctgccg aaatcatcta ctgaaatctc taccccttta tcccggagag tctccagatt
     4621 gttggtcagt ttatttagat ccgacatgaa aacagattcc gtcacttcca cttccaaaag
     4681 ttcactgggt actttgtagt ctttcaggcg ttttaaaata ttcggtacga agtctgtata
     4741 ttcattatgc cttctggaaa agttcaccga gatcggcacc acttcttctc tctgcgccaa
     4801 agcatcctgc aaatattcca gtacctgatc cagaatggca aaatccacct tggtcacaaa
     4861 tccgtttctc tcaaagacat caataaatct ccccggcatt accatagaac cgtcagcccg
     4921 gatccatctg accagtgctt cagcacccac tacttttccg gtgtgcaaag aaactttcgg
     4981 ctgcagatac gcccgaaatt ccttattatt atatgcagct accataccag atacgatctc
     5041 tctggtattc tccagattct tacggatctt ctctgtatag atttccaccg cactgtctac
     5101 cttctgggtc ttgcactgtt ttctggcctc attggcatta tccatcatgg aagacaccga
     5161 ccagtcattc tccaggattt catacattcc cgtggcaatg accagattgc actggtcgta
     5221 gcggctgttg gtcttctgca caaaagtatt ggtgaattcc agatatttgt ctctggcctt
     5281 ctccaccgta tcttccttca gcactcccac gaaatgatcc gaagttacac ggcagaacag
     5341 tacgccgttc tggcactttt cctgcaaggc tatcgcaaaa tcatgcagga tcctatctcc
     5401 cacttcataa ccgtacattt cgttcacata ctggaaattt gaaaaatcag atgacacaaa
     5461 aaacaatcct gatttcccgt gggcagccat ataggcctcc agcttatcca caaacttgtt
     5521 atatatcggc agtccggtca gagcatcata attcagtttc cggttcagct cacgctgatc
     5581 cttctccgca ttcttcagcg tgcgcagtcg gttaaaaatc tggtgagaaa ttctgcacag
     5641 tgcatttttc tcctcctgcc agcttctgtc tgtgtggcga ttcgagaaaa taacgctggc
     5701 ctttaatccc ttagcggccg tggacattac aatcatcacg gactccgcct cttccttgta
     5761 gacatccgga gtctctgact gccggtagat cacagcaccg gattcatcgg cctttttccg
     5821 gatcctgtcc cattcgaaca cgccgggatt caggattctt ctggcaaaat ctttcttctc
     5881 ttcacgattc cattgataac ggattgttgg tccagcctcg ttgacttcca tacagatcat
     5941 gtcatcaatt ccaaaaaagc tacaggtccg ctcacagatc acctttagtg cactggtcag
     6001 attcggcaca ttttccaaca gatccatgca aaataatacc aaatcttccg cactctcaaa
     6061 ctgtgcctct ttatgggcaa tctcgtcctc cttgttctct tcactgtcca gataactctt
     6121 gccgggcaat gccacacctt ccttgacatc cagcaggtaa taagcaccct ttcctttgct
     6181 ctttacctga tacagggctg catctgccca ctggaacaac agattgtaat ccgtcaccgt
     6241 gccgtctgtc accgccgcgc ccaggctaca ggaaatgtgc agccctgtct tgtcatcatc
     6301 ataacggtca gacatgcaac ggttcagctg ttttaatttc tcctccagct gttctctgtc
     6361 catgtcctcc acatagaaga caaattcatc cccaccgatc cggcctttta acgcctcggg
     6421 gaaaatatta ctgagttcat ccgcaaagga acacaatacc gcatccccaa acagatggcc
     6481 gttggtgtcg ttgatcttct taaagctgtc cacatcctgt acgaccagat acccagtctg
     6541 cccgcgtttc aggctctgta gcctggcacc gatctgttgt ttgatggtag aattgttcag
     6601 caatccggtc agggaatctt tctgagattt ttcccgcagt tcccactctt ttttcttctg
     6661 gtcatcaatg tcacagacct ttcccagaac ccgctccggt tctccctttt catcataaaa
     6721 agtctgtccg atgaccttca cccacctggg tttcccctct gcatcagtct tgcagagttc
     6781 gatataaata tccggcttgc cactgcgcag tgcttctgcc agctgttgtt ctaccgtttc
     6841 tgtccggttc ccggcttctt tgagcagctg atctgtatac ccttccgtga tctgccggga
     6901 atacagaatt ccctctcccg gaccggcata atacatcttg tcatttacaa tatcatattc
     6961 gaaaatcaga tctccggaca tacggatcac agccatcagc ttttccctct cccggtgcag
     7021 ttctctggtg gtctcatcca gctgtctgtc cacatcccct gtctgttttt cctgcaatgc
     7081 aacctcgttt ttcaatccca tacatttttc ttccatgcag accctctttt gctttctcta
     7141 tacctcttcc acccggctga aattccagac acggagattg tattccacga tcggcgtgtc
     7201 acagtaaata catttcttgg ctcccagtcc cggaagcggt gctccacagt tcggacagtt
     7261 cagtcccaga ccggcatcct cctgattctc gatcatatcc tgatcctgga tatagaccag
     7321 ttccacgtta tacttggcct gttctttatg ctcttcactg cctcttatta cttttccgtt
     7381 ctcttttaat gcatgaaaat actccactgc cgtctgcagg acaatactct gtcttcccct
     7441 ctgcttccgg tactggtgga tctccgttct gtgaatatgg atattttcaa agctctcctt
     7501 ctgggactgg ttctgtagca tttccagccg cagtctgagc tgttctttca attctctggt
     7561 tccctccgac aacagggccg gattctgttt cgtaatactc tgcagatagg aggtcagcac
     7621 attctccgct ctgctcttca tctcatcata atgaaattcc gggaaatccc gcataatgga
     7681 cggcatgtac aggttggttg cggaagagac cgacttcggt gtcatttcca cttccttttc
     7741 tctctgtttc attccctcga tcatggagtc cgttccgaat aacatccggg aggtatcccg
     7801 cagttttttc ctgatgttgc aataggcata ataaatgatt cctgcaagca ccagaagtaa
     7861 taccaatatg acgataattg caatcagatt tccgctcatt tatgtttctc tctgtttttc
     7921 attctgttgt aatgtttgta tagcataagt tcaggaacgc aggtgccaaa gttgcaccac
     7981 atagctgaac tgttaacagt tcagcgcgcc attcgcaaag ccgcactgtt acagcaaagt
     8041 acagcagacg ccaaaaggca tccgctgtac tgctgttttg tagaaatatt ctgctgagta
     8101 tgtattatac tctgtcgttc tcgtcaaaag gatcaccgca ctccgggcag aacttgggag
     8161 gatgtgcagg atcttccggc tcccagccac acttgtcgca tttatagatc ggtgcgccgt
     8221 ccggcttctt tgctccgcat tcggaacaga acttgccctg gtttactgca ccacagctgc
     8281 aggtccaacc tgcatcggag ggcttgggtg cgccacactc ggaacagaat ttgccccggt
     8341 tatccgtagc accgcagcta caggtccagc ctagtaccgg tgcctgcatc ttaggagcct
     8401 gcccctgcac aggtgctgcc tgtgcctgct gcatacccat accattctgc tgtgcatcca
     8461 tctggtacag ttggccggca ttaataccac ctaccatatt cgccatgttc ataccggcaa
     8521 atgccatcat aggtcctgta gaagtattag atgctgccat cttcatagct tcggcctgtg
     8581 cagcagtcat ggtagctgca gccattccgg tattgcgcag tactgcggtt ctctgcagat
     8641 cagcaatagt cttcgcatcc tcatcgggaa tctttgcaga gttgatattg atagtaacga
     8701 tctcgatacc gcgaagcttg gtccacagat cggaaagttc atcttttaaa gcctccgtca
     8761 gttcctcagt gtgtgcggga atctcactgg gacggacgcc ttttgcggag atcttggcca
     8821 gtgcgggctg caatgcagtc aacagttctg ccttcatctt cgctaccagt tcacttctgg
     8881 tgtaggactc tgttacgtta ccacatacat tcttgtagaa taacagagga tctaccagat
     8941 ggaatgaata ttcaccgtta caacgtaaag aagtatccag atccagtccg atattctgat
     9001 cgatcacacg gaaaggaatg gggcttgcag taccaaagag attgttcatg atctccttaa
     9061 tattaaaaaa gtagattctc tgatcctttg ccgcattacc tccgaaggta aaacgcttcc
     9121 cgatattgga aaatgtattc ttaacgctct caccaagatt tccgtagaac aggctgggct
     9181 cggtggaaga atcatatacg aattcacctg catcggcaca gaactctaca attccgccct
     9241 gatccacgat gatcatacac tgtccttcgt tgactgccac gatagagccg ttggagataa
     9301 tgttctccac acccttggtg ttactgttac gtccattggt tacacgctgc tgtcctttta
     9361 ctaccagcac atcattggaa agggaatcac aatagaaata ctctctccac tggtcagcca
     9421 tcatgctgct gactgcatct ttcgctgctc tgattaaacc cattaccgca cctccgtttt
     9481 tatatgaaaa atctgttctt tttcacatct cttatatttt agcaaatttt acacattccc
     9541 tcaactgctt ttcgtaaaat atcgtctttt ttcctttaca atgtgccgtt tttctactaa
     9601 actgtgatac tagagatgtt tgattaaact ataatataaa agggttgaaa gaaaaatggg
     9661 taaaaaagct acaaaagccg ctgataatat gtactatctc gcacgttgcg aagccgctga
     9721 gacgaatgcg gacttcagca gccgggaaaa agccgccgaa ctggtgggca tcgaccgtac
     9781 cagactggcc aggatcgaac tggacaccat tgttccctat cccgaggaag tcaaggccat
     9841 ggccaaggcc tacaatacac cggagctgaa caattccttc tgtgccaggg aatgtcctct
     9901 gggccgcagc agtgtatcgg aagtcgacat tgtggacttt gaccggctgg cattgaaggt
     9961 actgggttcc ttaaaagata tcgacaccct gcgggcttcc ctgattgcca tctccgagga
    10021 cggcatcatc tccgaagatg agcgcccggc cttccaggat attctgaatt ccctggagaa
    10081 gatcagcacc aacgccaaag ctctgaagct ctgggccatc aaaaatatac acatcaagga
    10141 ggaggacgtc tgatcctcct ccttttttac aactatcctg ttttatttat cttgccgccg
    10201 ctttctctgc aaattccgta gttaccagtg tttcgtaagg tactctctct ttcagcacat
    10261 ccgaatcctc cagaatattc tgcaacaggt caaaggcatc tttatggaag atcagatcct
    10321 ctttccaggt atcctgttcc tgatatctgg caacaatagc tgtcaatgtg tcactttcgg
    10381 tctcggcaaa ctggggtgca atacacttgg caatttcctc cgcactatgc tcctgtacnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc gatggacaga tccgttgtcg
    10561 gatccatatt atttttctcc agaatatatt caaataccat ctccggcata ccgcccgctc
    10621 taccgcccaa gacggtagta ccttttaaca tatcccacga aaaatcatcg atgggttctc
    10681 tcgccaccag gaaattgcca gcccgctggg tcagctgggc aaaattcacc acataatctt
    10741 ccgttccgcc agcataggtg taaatggtgc tctcgctgcc cataaagccg atgtctgcct
    10801 ccccggtcag tactgccgtc atggtcttat ccgcgccaaa tcccgtcacc agctccagat
    10861 cgatcccggc atcttcaaaa tatccttctt cgattgccac atacatcggt gcataaaaaa
    10921 cagagtgggc tacctcattg agcaccactc tggtcttctt tggtccgttc gtctgctgtg
    10981 cgctgccgca gcccgtaagt gcagccgctg caagcactac agacaatact gctaccgtta
    11041 ctttccttct tttcataatc attcatcctc cctgagatta catatttatt ctatgtaacc
    11101 tcagggaaaa cgttccatat aattacagat ttgccttgat cttatcgatc atctcctgat
    11161 actccgcatc ggtcagcaga gtcttgccgg ggttcatctc gaaggttccg ccccactcga
    11221 agccgtcctt gtacttcggg cacaggtgca tatgcagatg gcagccggtg tcaccgtaag
    11281 cgccgtagtt taatttatcg ggatggaatg ctgcatgaat agctttggct gcatgagcca
    11341 catcctcgaa gaataatctt ctctcttcct cgctgatgtc cacaatctcg ctgacatggt
    11401 ccttatatgc cacgatacat ctgccgggat ggctctgctc cttgaacagg atcaggctgc
    11461 ttaccttcag atcacagata aaaatgccga acttgtctaa cagttctcct ctcatacaat
    11521 atccacaatt tgcgtctttc atttgttctc ctatctgccc tcttacgggg cctgccgtct
    11581 gcggcttaaa ttcttgtccg gtttgccggc caggatcagc tataatgtta tgttgatcag
    11641 cttacggatg ttctccgtat catagttcaa caccagttcc ttcggaaatt ccagttcccg
    11701 catcagttgc tctgcatcat caaatgctcc gatggcaaag cacatatggc tgtccgttcc
    11761 cattataaca ggctgatcat acttcatgca agtctttaac agctctgtaa tattttctct
    11821 gcctccttgt ctggccgact ggggattcag ggatgaattg ttcacttcca acaccgcctg
    11881 tgcctgtctg gcctctcgca ccagttcgtc ataatccaac ggaaatctac cgtcatcggg
    11941 atgcccaagt attttcacat aaggattctg aaaagctctg acagatgcca gtgtattctc
    12001 cttgcggctt cccgcagtaa aggcaaaggg atgaatgctg gcaagggcat aatccatttt
    12061 ctgcagatag cgctcttcca gatccagttc accgtttgta ctgcagatat ttgcttctac
    12121 gccgcatagc aggcgcagac ttccatctgg cttcggtatt acccggccgg tgttctcttc
    12181 tctgtcatat attctgggaa tcactttata attactgaaa aagaaattat gcgggcctcc
    12241 cggggaagtc ggtccgtgct cactcattcc caggtactgt aagcccattg ccaatgcagc
    12301 atctatattc tccaacaagg tactatacgc atgccctgct gccagagtat gcgtatgaag
    12361 atccatgatt gctttcattc tattgtcctt ttactttcca aaacggtcta attctttttc
    12421 ttatgagctt ccttctccgg ttcctgtgct ttcatcgtat ctgccagatc cgctaccagc
    12481 tgttccacaa tcatcttctt catatagaca tcaaagctca acaggcagat cagggcaaat
    12541 ttctgtaaaa actcctgctc ttctccggtc acttcggtaa aggaatcctc tgctctgcgg
    12601 aatttctcca caacatcctt tttcagatcc tccagctgtt ccttctccag caccaccact
    12661 tccttgtaga cgctctccag gtcaaagacc tgtccgttct gaaaatagcg gtcagtaagg
    12721 ggcttaagca gcgtctggat ctcattcatg ggaatgatcc ctttataata gtagataaaa
    12781 ataagaagca gcacatgctc tttggaatat ttcttcttca ccggcggcgg cagcagatca
    12841 ttcttcgcat aattattgat catggtcttg gtcaggatgc ggtcttcggt ctggggccgc
    12901 actgcggaac gcagcttacg atccataaat gtcagtacct gatccatata cagatcaata
    12961 ttcggaatgt cttccgactt gatatattct actttctcca gactctgtaa gatcatctga
    13021 agtaattctt ctgttgttgc tgccataatg ttcctgctgt cctttccgtg aggtagtttc
    13081 caaaaatatt gactcgagaa cccgtgcttc gcactctgct gtctgcttcg cagacggttc
    13141 tctcgttaat gcatgcaaga aaacaaatgc tcgctctgcg agcgcttgtt ttcttttgca
    13201 ttcattatat agtattcaaa actacatgtc aacagttgca catttcctct gttctctgaa
    13261 tttatgaaga ttttataact attttgtttc actcctttac tttacaactt tagtatgtta
    13321 aaatactaat agttgcgttg tattgattac aactatggaa aggtgtgtcg ctaatgaata
    13381 aaaaaatcaa aacggacgct gtagattccc tttttgatgc ggtgttgtgt ctgcagtccc
    13441 gtgaggaatg ctacagtttc ttcgaagatc tgtgtactgt caacgaactg ttgtctctgt
    13501 cccaacggtt cgaagtggct gccatgctga aggacagcaa gacatatctg gagattgccg
    13561 agaagaccgg agcttccacc gccaccatca gccgcgtaaa ccgttctctg aactacggca
    13621 acgacggtta cgagctggtc tttggacgta tggagaaaaa ggaaacggaa cagaattctt
    13681 aacttaatga ataaaaacag gagcactcaa aaacggcgct cctgttttta ttttatcttt
    13741 tattcttctt atgcttcgct tgtattggct gctgttgttt ccgcagctgg agtttctgtc
    13801 ttctccaccg tctctgctgt ctgtgaagtc ttctgatttt ctctcttttc ttcctcttcc
    13861 acttccttca gagcttctgc gatggctgcc ttggcactct tgcgtcctgc ccggaccgct
    13921 ttggccgttc tggctttacc ttttctgtca ggaactgtct ggttcatatc gatttttccc
    13981 tggaatacgg catgttcatc aataacgatc acctttgtgg caatgtcccc cagcactttg
    14041 gccgaagctg tcagttccgc tttctccggt gcagtgatat ttccggtcac ttccccgccg
    14101 atcagaacag acgcagcctg tacatctcct gtgactatgc ttccggctcc caggatcagg
    14161 ttgcctgtca ccgtcacgtt accaccaatc ttaccgtcaa ttcttgcaga accgtccaat
    14221 gtaaaatctc cggtcaactc cgctccggca ccgatcagtg tgctcatttt tgtatctgca
    14281 ttcttttttc ctaacatgta tcttccaaac ctttctgcaa tcaaccactg attgccagca
    14341 tatccatagg attaatatag cttccgtctt tctgcatctg gtatcccaga tccgcgttat
    14401 cttcgccgat gacaaacaag gtagttccct gtactacgga atcctccaca ttcactgtgg
    14461 tatctccttt attccgatag atggtgacat aaccgttgcc atgatccacc cacacgctgt
    14521 gtccgtagat ctcgtcatca tttatggcaa tgaccgtacc gctggccgcc gccacaaccg
    14581 tagttcccac cgatgcatgg aatatgcaca tgggatcgcc ctcacttact tcctccatgg
    14641 atgcagaacc cgtcagaggg aattccgtag gaaggctctg cttttccaga gttgctgata
    14701 attcgctctc actctgtacc ttctggctta cggtgtcgct taagatctgt atctcttccg
    14761 cctgttccgt cacctgctct tccagcttac tgttctgctc gctcagtgtc ttgttctctt
    14821 cctccagtgc cataacctgt tcatggctcc gtctactggc gttctcccag agctgtccct
    14881 cgtagacaaa atagccgatc actccgccga ccaatacaca caacagaagt accacgactt
    14941 ccacgatcca tgcacgtaca cggaactgct ttacattggc atctgctgcg tctgacgtaa
    15001 gaatcagcac atggttcaat cttcgcttat gtttacgtct tttcatagag tcacctccgc
    15061 taaatatcaa cccattttac cacaggaaag ctcttacagc aatcatttct taacattttt
    15121 gtaatatttt cccaagattt tcttcattta ttcgtattta aaaggaaatt gcctataaat
    15181 ttttgtgcaa ttttacagaa tcgtctattt acttttacta cctgctgtgc tatgatgcac
    15241 ttgctgagac aatgttgtat acattgcaaa aagtaaatga cagcaaagaa tttgtggagg
    15301 tatctattat gaacaaaggt acagttaagt ggttcaacaa tcagaagggt tatggtttca
    15361 tctccgatga atccggcaag gatgtattcg tacactacag cggtctgaac atggacggtt
    15421 acaagaccct ggaagagggc gcagccgttt cttttgatat cgtagatgga gaaaagggac
    15481 ctcaggctgt taacgtaacc aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnacatcttc ccatttcatg ctgccgccga agagatccag tgcactcccg atggtcacat
    15661 tcatccggtt ctgccccagt ttttttaaca aatacagatc ttccatggag tgaacaccac
    15721 ccgcataggt gattggattt ccggtataat ttccaataaa ctttaccagt tcctgttcca
    15781 ttccccgggc cttgccttcc acatccacgg catggatcag gaattcatca caatagacag
    15841 ataatttttt aagtagtgcc tcatccactg cttcttctgt cagcttctgc cagcggtcag
    15901 tcactaccag atactgctct cccgcacgct tgcagctgac atccagcaca aggtgctctt
    15961 ttccgatgga ctggaccagt ttgtccagtc tgtcatagtc gatccgtccc tccctgaaca
    16021 cataggacgt cacgatcaca tgggaggccc ctgctgccag atactctgcc gcattctccg
    16081 ccgtaatgcc tcctccgacc tgcaatcctc ccggatatac acgcagggcc gacaatgcct
    16141 gttccttcgt tgcctcataa taagggctgt ccgaggcatt caataaaata atatgtccgc
    16201 cttttaaact gtttttctga tacagttttg cataaaaggc agcgtcctgt tctgagacaa
    16261 agttctcctg cgcccgatca ccgagatctt ttaagcttcc accgacaatc tgttttactt
    16321 ttccgttatg aatatcaata caggggcgaa attccatctt tttcctcgtt ttcttccaaa
    16381 tgatttttat ttttttgaat tattttctaa aatccgcaca tactaccctc aagccgacca
    16441 cctttggttc ggactgaata tccctgacgg gcatcttgaa atggaggaca ttatgaaaaa
    16501 taagacaaat aacgtaacat cccacatcgg gaaaaaatta ctggttctgg gcatcatcac
    16561 agtctacctg tgtatggcga ccgcctgtgg caccaacaag aacaacaata ataactccgg
    16621 cagcatggct gcctctcctt ctccgtccac ctctcctgct gctcagggga gcacagaggc
    16681 gcagaccaca gagaccggaa ctgccgctac cgacagcact atgggtgaca tgaacggcaa
    16741 cggtaacacc gtaggagaaa atgattccat gctggacaat gcaggcaatg ccatcggaga
    16801 agccggtgat accgtaggca atatcgtgga cgatgccgca aacggtgtct ccgatatcac
    16861 caacgacctg gtaggcaacg gtaatgatac tgcaggtaac aacaattcta ccactgccac
    16921 caccggagcc ggccagagat aagttatacc tcttccatga tcgtaccttc ccggtaagcc
    16981 tccaggagtt cttccagata gaagatattc tcctggatct ccttgccggt atcggtatcg
    17041 gccaccagtt ttctgcgggg gaaattctcc accaccacgg aatccgagaa aatatccgat
    17101 tccttgatga tcgccgcttc cgtagattca aaaggttcat gggctaccag acgcattcct
    17161 ctggaattac ataccagcgt atatcccgca attcccgtct ttttctgata tgctttggaa
    17221 aagccaccgt cgatgatcag cagcttaccg ccgcatttga tgggtgattc cccatggatc
    17281 tgttccacgg gcacatgacc attgacgata tgggcatcgg ccttgtccag accgaattcc
    17341 tctaagattc tgccgatgac ttcctcattt tccaacagct tatagtaact gtttttctct
    17401 tctatatgag tctccttatc cttcaggaaa taccgctcaa aggtagccat ttttgccttg
    17461 ccgaacaccg gggaaccggg gcctgcccag atgtaccaga tcatatcctg tcccagagcg
    17521 cgctctttgg cttccttggc ataataacct cttctggcgt aatattccag aatgtcgtac
    17581 agtgcttttc cgcaatactc ttttccgcag atctccacct gcatgaaact gccgtccgga
    17641 ttcaagggga cacagccgtg atacaacaga ttgccgttgt aaatcttgta catgccgccc
    17701 ttggaaaaca gaaattttac atggcgttgc agtttctcac agcgcatgaa cacctgctgc
    17761 agacgcagca tgaccttgct ctcttcctcc gtgagttcgt aaggatgtac aggatccacc
    17821 gtagggaaat ccacgtcttt catgggatat tctttgccgt ccacgcagat cgtctttttc
    17881 tcgaaatcga tccgatgcaa cagcatccgg tcttccatat ggaactccgg gtgtctcatg
    17941 atcagctgtc cttccagctt gaactgcaag atagcaattg ctttatgcat cttggtgtcc
    18001 agcccgagat ccttggtatt gtaatcggta ttgaacttga tggtaaagac atcgcagttg
    18061 gtattctcat aagtttccag tgcaaatgta gccagaggca gcaggttgat gccgtagcca
    18121 tcctccagag tggcgagatt accgtatctg gcagacattc tgaccacatt ggcgatacag
    18181 gccagacttc cggccgccgc gcccatccac accatgtcat ggttgcccca ctggacatcc
    18241 acggaatggt attcgcacaa agtatccata ataatatgag ggccgggacc tctgtcgtaa
    18301 atatcaccga cgatatgcag atgatcgatg accagccgct ggatcaggtg gcttaacgca
    18361 ataataaact gcggcgctct gccgatgcgg atgatagtat ggatgatctc attatagtat
    18421 gcctctttgt cctggatttc ttccttctcc gtgatcagtt cttcgatgac ataggcgaaa
    18481 tccttcggca atgcctttct gactttggag cgggtgtatt tggaagagac acgtttgatg
    18541 acctgtacca gacgatgaag ggatattttg taccagtcct ccagattgtc ctcttctctt
    18601 tccacgatct ccagtttgct ctctggataa tagatcaggg ttgccaggct ttttttgtcc
    18661 ttgttgctga tggtgttgcc aaattcctcg tcgatcttgc ggcgtacgga accggaaccg
    18721 tttttcagga catggttgaa ctgttcgtat tctccgtgga tatccgtcag gaaatgttcc
    18781 gtacctttgg gaaggttcag gatggactgg aggttgatga tctctgtgga ggctgccgct
    18841 atatttttgt attggtgtga gagggtcttt agaaccttct gttccatttc gctcatgaaa
    18901 ttcacctttc tatgttactt cacgaaacgt acggatacca tgcctcggtg taaggtctcc
    18961 gagtgtgaat tttttaggaa aagtgattca tttgttctac ttaacacggg cacgcctaca
    19021 cgcaccatcc gtagtgcgga tcggctgtcg caaacatcgt gtttgctcca ccctgttctt
    19081 caacactttt caagaaaatg tcaacactct acgacaaaca tcatcgccat ggcatccatc
    19141 cacttcgtca gttcatgact ttaccaggta attacatcgc tgcattgata acatcagaca
    19201 tgttgtacat gccggcgggc ttgcctgcca ggaacttggc tgcctgtacg gcgcctttac
    19261 cgaagacagc tttggagtag gcggtgtggg agaaggttac cacttcgtct gccccggcga
    19321 agatcacatc atggtcaccg acgatggtgc cgcctctgac agagctgatg ccaatttctt
    19381 tcttcggtct ctgctctctg cggttgctgc ggtcatagac atattcatac tcaccgttaa
    19441 attcctcgtt gatggaatct gccagtgcca gtgcggtgcc gctgggagcg tctaccttca
    19501 gattgtggtg cttctccaca atctccatat cgaagcctgc gttggccagc actccggcag
    19561 cttctttcaa aagctttaac atcaggttga tacctaagga catattggcg gattttaaga
    19621 tagccacctt tttcgctgct tcgtctactt tcgccagctg ggcctcggac agaccagtgg
    19681 tgcacagcac gcagggaagc tttttctcca cgcagtaatc caacagcttg tctactgcca
    19741 cggctgcgga gaaatcaatg attgcatctg cagccacatc acatttgtaa atatctgtga
    19801 atacagggta gggattgtgt ccatcgtctc tggcatcgat tcccgccacc agttccatat
    19861 cggggtccgc tgcgatcagt ccgctgatca cctgtcccat tttaccgtta catccatgca
    19921 tgattatttt taccattttt cttttctccc atctatcatg atttttgtaa taacgatcca
    19981 gcagccctca tattcatttg caaaagcgca ctatgaatcg ttactttttg aaaaaagatc
    20041 ccgcccatga taaaattttc acaggcggga ttcttccact cttgctctct attttccagc
    20101 ctacaggaca ccataatcct tcagggcttt ttcaagcctc ttggcattgg cctcttccat
    20161 ttcacacaga ggcatacgga acgcaccggc tcccattccc atcatgttca gtgcggtctt
    20221 cacagggatc gggttcactt cacagaacag tgcgttacac agatccatgg catctaactg
    20281 catctgacgg ctacctgcca catctccgtc aaagaacttc tggcagatct cgtgggtctg
    20341 tgtaggtgct acattggaaa gtacggagat aactccgact ccgcccaagg ccagaatggg
    20401 tacgatctgg tcgtcattac cggaataaat atccacaacg ccctgtccga tggctgccag
    20461 gtgagcgatc tggctgatgt tgccgctggc ttccttgaca cctacgatgt tctccacgtc
    20521 cttacacagc ttcactacgg tctccggcag aatattgcat ccggtacggc tgggtacgtt
    20581 atacagaata gcgggaacct tcacagaatc cgcaacggtc ttgaaatgac gatacagtcc
    20641 cttctgggtt gctttattat agtagggact taccagcaga atgccgtcca cgccgtattt
    20701 ctccgcttcc tgagacagat atactgcggt atcggtacaa ttggaaccgg tacctgctac
    20761 tacggggatt ctctttgcaa ctttgtccac acagtatttg atagtatcca gatgctcctc
    20821 atgggtcagg gtagatgact cacctgtggt accacatacg ataatagcat ctgtgccgtt
    20881 tgcgatctgg aattcgatca gttcaccgaa tttctcataa ttgacggatc cgtcctcgtt
    20941 catgggtgtg acgattgcca cgcctgctcc tttaaaaata gccatatgct tcctccattt
    21001 gcaaaacgcc agcgcacaat gacaatggcg ccggcgctga ttatccaagc ttatttgata
    21061 gcgccccatc tgctcacgag atgacagtca tacgcttctt aaacgtatgc ccaagataac
    21121 atatacagga taccttatct ttcggcgtgt tcccctttct tcttccctca tcggtacctg
    21181 ttacctccga aaaaatactc ttgaaaaacg caacctctat cttcttaaat atagttttat
    21241 gatagcatca acccatgtga ctgtcaatcc cagaccttga attttaccgt ttcaatcttg
    21301 gacacttcat ccgccagaac acagatctca tcgttcaggg tgattcccgt cagaatcaca
    21361 tccgtatctc cccggcattc cagtaatgcc ttcaggtcat cttccgtaat aatatccttc
    21421 tccaccagtc ccagcacttc gtccaggatc agcagatcgc attctccagt cgtcagtact
    21481 ttcttggcaa agccgatccc gttacggatg ttctggatct cctcctgctt cttttcctcc
    21541 ggtaattcca cgaaattacc gtccgatttc tcgaaacgga acagcttgat ctccggctcc
    21601 attctgcgta aaaaatcgga atcccccagt ccctttccct tcaggaactg aataataacg
    21661 accctcttgc cttctaccgc tgcctgtacc gctcttccaa tcgctgccgg tgatttgcct
    21721 ctgccatcgc cggtataaat atagatgcat ccttctgcca ttccacacct catggtctat
    21781 ctgcgtatct gacgctccct agctgtacac agttactaat atctatgcat tcttttatac
    21841 catatattgt cactttttgc aatcttttgt cgttagacga tggattcctg cacctgtgcc
    21901 tcctccagaa gttccagttt gctgacggat acctcgaaag ctactctttt ctccacatgc
    21961 tcttcatcca gttttttcat atattcacgg ctctggatcc gtccccagat cgcacagcgc
    22021 tctcctacct tgaaatgctc cgcataacgg gcgttacgtc cccaacagat acagggaata
    22081 taatcggatt ttccgtacgg acggtttacc gccagcagca gatccgctat ttctcttccc
    22141 aacggtgtct tacgatacac cggttcctta catatatatc catccagata aatctggttc
    22201 gtcttcgaac tttcttccat ttcttcaata aatttcactt ctctggcaaa cacagacaac
    22261 accagtctgt tcttgcgctc ctcatgacga ttgtaggaac gaaactgtcc gttcacacac
    22321 aaaagcattc cgacatagct ggctgtcacg tccagtaatc tttctgagac catcaccggt
    22381 ataatgtcaa aactgtcgct cagtctcgta catttgatgt ccaccatgta gaatccttcc
    22441 ccatagatct catggctgta ggaaaaattg cttaccactt cccccatcac cgttacctga
    22501 ttgttttcaa taaccttatc tgtcattttt gttctccctt ctacatcttc tgactcttct
    22561 acaacaatct ttatgtattg cactactatt tatactctaa aatccatcgt ttgagtacta
    22621 aaactcatcg cacaaaaggg caaattacgc acaaaaggga tttcttttcc atactttttc
    22681 atatagaaaa ccaccaaaag cgttgatttt ccacggtttt ttcttgcgtc cggaaatgat
    22741 aagtgctata ctgaaacagt ttgaaaaaag attgaatcag ttgcacgcat ttttagaaag
    22801 gcaaggattt ttatgagaca aaaaagagaa gatgtcagaa acgttgcaat cattgctcac
    22861 gtagaccacg gtaaaaccac tctggtggat gagctgttaa aacaaagcgg tgtattccgt
    22921 gaaaatcagg aagttgctga gcgtgtcatg gactccaacg atatcgagcg tgagcgtggt
    22981 atcaccattc tgtccaagaa cactgccgtt atgtataagg gtaccaagat caacatcatc
    23041 gacaccccgg gccatgcgga cttcggcggc gaggtagagc gtgtcctgaa aatggtaaac
    23101 ggtgttatcc tggtggtgga tgccttcgag ggtgccatgc cccagaccaa attcgtgctt
    23161 cgtaaagcac tggaattaaa gcttcccgtt atcgtatgta tcaataagat cgaccgtccc
    23221 gaagcaagac ccgatgaggt cgtagacgag gtactggaac tgttcttaga gctggatgcg
    23281 gatgattctc agctggattg tcctttcgta tatgcttccg caaaggccgg tattgcctct
    23341 ctggatgctg acgaaatggg tactgatatg gaacctctgt ttgagaccat tttaaactac
    23401 atccccgctc ccgaaggaga tccggatgcg gctacccagg tactgatcag taccattgat
    23461 tataatgaat atgtaggacg tatcggtgtc ggtaaagtag acaacggtac catccgtgta
    23521 aaccaggatg tggtcatctg taaccaccac gatcccgaca agatcatcaa gaccaaggtc
    23581 agcaagctgt acgagttcga cggtctgaat aaagtagaag tcaaagaagc tggcatcggt
    23641 tccatcgttg cgatctccgg catcagtgag atccacatcg gtgacaccct ctgctctccc
    23701 gaaaatgtgg ttcccattcc tttccagaag atcagcgagc ctaccattgc catgcagttc
    23761 atggtaaacg attctcctct ggccggtcag gaaggtaaat tcgttaccag ccgtcacctg
    23821 cgtgacagac tgttccgtga gttgaatacc gatgtttcct tacgtgtgga agagaccgag
    23881 tccgccgacc gtttcaaggt atccggccgt ggtgagctgc atttgtccgt actgatcgag
    23941 aatatgcgtc gtgaaggttt tgagtttgca gtaagtaagg ctgaggttct gtatcatacc
    24001 gatgagaacg gcaagaagtt agaacctatg gagctggctt acatcgatgt tcccaatgaa
    24061 tttgccggtg ctgttatcga gaagcttggt cagagaaaag gtgaacttcg taacatgggt
    24121 tccatttccg gcggcactta cacccgtctg gagttctcca tccccgcaag aggactgatt
    24181 ggttaccgcg gtgagttcat gaccgatacc aagggtaacg gtattctgaa caccgtattc
    24241 gacgactacg gcccttacaa gggagatatc cagtatcgta agatgggttc cctgatcgca
    24301 tttgaagccg gtgaagctat cacctacggt ctgtacaatg cacaggagcg tggtactctc
    24361 ttcatcggac ccggtgaaaa ggtatactcc ggtatggtca tcggtcagac cggacgcagt
    24421 gaggacatcg agatcaatgt ctgcaagaag aagcagctga ccaacacccg ttcctccagc
    24481 gctgacgaag ctctgcgtct gacaccgccc aaaatcctgt ctctggagca ggctctggac
    24541 ttcatcgata ccgacgaact gttggaagta actcctacca acctgcgtat ccgtaagaag
    24601 attctggatc cgaccatgag gaaacgtgcc atgatcaata agaaagctta agaagattta
    24661 taaatagaac caccttcgtg tactgcctgc agtatgtgaa ggtggttttt aactgggtta
    24721 acaaagaatc ccaaatgccc acggcgaacg gaacatcctt cctcgatgta aggtctcccg
    24781 gtgggaattt tctgacgaaa agtgagtagc ggttctaaac cgcacgggca cggctccagt
    24841 tgccatccat ggcaacgtcc gcctgtcgcg aacgtcctgt tcgctccacc ctgatgttca
    24901 acacttttcg agaaaatatc accacctccc gacaaacatc ttcggaagga cattccatcc
    24961 gccgcgggca ttctgcgttt tggaaagcct gcgaaaaaac agtagcccgc gttggggtaa
    25021 ggacaagcag actctaagca gccgtcggta cttagcgagg gtactttcct cgcttatgta
    25081 ccgctacggt ctgcgtccga agtggaaacg ggtctgcggt tcttagcccc aatacgggct
    25141 acggggatat ttcactaact cttcagttaa ttccaggtgg ctgtcaacac atgctccccg
    25201 gtcatctgga cgacggtgtc tgcggtaata atctggccgc tgtactccca tccgccgaag
    25261 gcactgccct cttttaccgg ggtaggaagt tcaccgtagg tctcgccgaa ggtcacctgt
    25321 ttggattcca cggtggaacc gtcggcaccg gtgccgccgt catagtcgaa tttcacggtg
    25381 aactgtgcgg gaacccagtg agcggtcaga gtcatatcgc cggtgacttt ctccgactgg
    25441 aaataccaca gcactgtctc tccctctttt tccgtatacc acccgtcaaa ggtgtagccg
    25501 ggcttataag gggtctcggg ctctgtaagg aattcaccgt agcgtacctt ccgggctgcc
    25561 acttcacttc cgccgtccgt atcgtacaca atgttgaatc caccgcgtac cgcgaagaaa
    25621 ataatcattc ccacgatcac cacgatcagg acaccgatca ggccgtccag aatgcggatg
    25681 ggaacatcct tgcttccgta aatgccgcgg ccgaaccatt ttcctttcgg acggattgct
    25741 tcctccggct gctgcgtcgt ttccgactgc gtggtgactt ctgctgcctt ttcttctgta
    25801 ttctctgttg tcacttgttc ctgattcata atttttcctt ttcctaatct atcataagca
    25861 atttctatgt gctgcgcact ctcgaaattg caaccaatat tcgtattccg gactgtcgtc
    25921 cttcatactc atattgtttt actcccaaca tagaaaacat cctttacctg caagcagtca
    25981 cggatgtttt ctatgggtga gtcttatatc attactttct taccaggtac tttctcatat
    26041 cgatcgcgca ggcaaccaga atgatgagtc ctttgatcac atactgcatg ttcatgttga
    26101 tggacaggaa gttcagtgct acgaagatga ttcttaagag aaatacacca agtacgacac
    26161 cactgatctt accggtacca cctacgaaag agacaccgcc gataacgcaa gctgcaatgg
    26221 catcacactc gtagttcaat ccggtatttg ctgtgttgga accgatacgg gaagactcga
    26281 tgaaaccggt cagaccgtac atggcacctg ccagtgtatg tactaaaatg gtggttttca
    26341 ttacgttaac accggatacc tttgctgctt cgggattgga acctactgca aacatgttct
    26401 taccgaaggt ggtcttgttc cagatgaacc acatgataac aacgatcaaa gctgccagcc
    26461 atacatagtt gggaatggcg attctcgcgc cgccggcatt gaaggagatc acgcttccct
    26521 ttaccacatc gttgaagtgc tgatccagac cggacagagg ctgaccgtta ttgccgttga
    26581 tcatgatgta cattaacagt aggccgtata cgatcaactg ggtagccaga gttacgatga
    26641 agggatgcaa ctggaatttc gctacgaaga aaccgttgac ccatcccacg ataccaccga
    26701 ccaacagtac gatcaggatc accagaggga tcggaagtac cggaaggttc gggaacatct
    26761 tggtggcata agtcacggac tgcatcaggg atgccgtgat acatgcggtc agtcctacca
    26821 cacgtcctgc ggacaagtcg gtacctgtca atacgataca tcctgcaata ccacaggcaa
    26881 tgggaaggtt cgccgcggac aggctgatga tgttgacaat ggaagccgcc gacaggaaat
    26941 tggagttctg gatgtaagtg taaataattg ctacgatcag caggatgtat aaggcattgt
    27001 taagtaaaaa ttctttccaa taggtcttct tgtctctgct gtccagcttc ttgtagtctg
    27061 caaaattttg ctttacgccg ttaattttat tctgaattgc tttgccggga ttcatacgca
    27121 tatcctccta tacatatttt gcagcaagag acatgatctc ttcctgtgtc gtatttttgg
    27181 catctacttc tcctgccagc ttaccgccgc tcatgacaag aattctgtca catacaccca
    27241 gcagctccgg catctccgaa gataccatca ggacaacttt gcccttattg gccagatcga
    27301 tgatcagctg gtagatctcg tactttgcac ctacgtcgat acctctggta ggctcatcca
    27361 gcatcagcac ctccggctct gtgagaagcc atctgccgag gataaccttc tgctggttac
    27421 caccggacag tgaccggatc ttcgtcttct gggaaggtgt cttgatatgc atggcatcaa
    27481 tggcccatgc agtgtccttc tccatggatt tatcactcag ataaaatcct gcttttttat
    27541 gtttcttcag actggagatc accgtgttct cccggatatt caggattccg aagataccgg
    27601 tggcacgacg ttcctccgtc agaagtgcaa aaccgttttt aatggactct ctggcattgc
    27661 ggtttctgac ctctttgccg tccagtttga tcacaccgtc ttttctggtg gccacaccga
    27721 agatattctc cagtgtctcc gttctgccgc tgccgtccag tccggccaga cctacgatct
    27781 caccctttcg agccttaaag gatacatcct gcagcaagga atatctcgcg cttaagtgtt
    27841 ctacctccaa tgccacttca ccgggcttgt tggtcttggg cgggaactgg ttggtcagtt
    27901 cacggcctac catcagtttg atgatctttt ctgtagtcag gttctgtgca ggctctgttg
    27961 cgatccaggt accgtcacgc atgacggtga cttcatctgc gatccgcagg atctctgcca
    28021 tcttgtgaga aatatagata ataccgcatc ctctgtcacg aagcatattg atgatcttga
    28081 acagatgctc cacttcttct tccgtcaggg atgaagtagg ttcgtcaaat acaatgatct
    28141 tactgttgta ggataccgct ttcgcgatct ctaccatctg cctttgtgat accggcatcg
    28201 tactcatgat tctcttgggg tctacagcga tccccagttc ttcaaaaatc ttgttggtgt
    28261 cttccagcat cttgtgctcg ttgaccataa tgcctgctgt catgggataa cggcccagcc
    28321 agatattatc catgacattt ctcttcagtg cctgattcag ttcctggtga accatggcca
    28381 ctccattatt cagtgcttct ctggaatttt tgaaattgac ttccttgcct tccagttcga
    28441 tcttgcctgc atccttactg tagatgccga acagacactt cattaaggtg gatttaccgg
    28501 cgccgttctc tcccataagc gcgtgtaccg ttcctttttt tacggtaagg gatacatgat
    28561 ccagtgcctt tacgccgggg aattccttgc atatgtctgt catctgaagt aatacttcct
    28621 gtcccatacg catcctccta tacataaaga gcggccgctg ccagatgcag cagccgcttc
    28681 ttttatttac ttgttattca gatcattctc cggtgtaagt agcgtaaggg atgtagatct
    28741 tgttgctaac cttctcagag atgttgaagc tgtcggtatc tgccatcaga tccttgccag
    28801 cctggatgtt cttagccagg taagcaatac cgtttgccat accttctgca tcctgcttga
    28861 tggtaccggt catcttgccg tctgcgatca gctgcttggc tgcgtcagtt gcatcaacac
    28921 cgaatacagg aatggtggtg cagttgccat cgcctaagtt gtagcccttg tcattcagag
    28981 cggagataac gccttcagcc ataccgtcgt tgttgcagat aaccagctcg atcatgttac
    29041 cagcggactc attgtactga gacaggttgg tggtcatgta gttgttagct gcggttgcgg
    29101 accagttacc gtcctggtca acctgatact tatcggtatt ggatgcatcg aagtactcca
    29161 gagcaggctt gcctgctgcg gtgatcactg catctgcatc ttctacaccg tactgggtac
    29221 gatagattgc ttctacgtta cctaactgac ccatgaacat tgcatagctg atcttgccgt
    29281 cgccgtttaa gtcgatggtg tcaaagttgt ctactacata ctgaccaacc atcttaccct
    29341 gcatgtgacc agcttcggga gcgtcggttc caacgaatgc acacttgtca tagctgccta
    29401 ataccttacc ttcatcctca tcggactcta ccgcacggtt gaagaagata acggggattc
    29461 ctgctgcgct tgccttgtca acgatggcct gggaagcgtc tacggaaccg gatgttacga
    29521 tgttgacgat cagaaggttg gtaccctgtg caatagcggt atcgatctgc tcgttctggg
    29581 tggtctggtt actgttgccg tcataattct ggaacttgat gcctgcttcg gtaagcgcat
    29641 tatccagtgc agtacgcacg ctggagatgt atgtatcact gtaggtgtaa tagaatactc
    29701 ctacattcaa ctctcccttt cctccgccgc tctttccgca cgctacgagg ctgagcatca
    29761 taatgccgac caacagtagt gacatgaact ttttcatatg aaatacctcc ttgttttgat
    29821 gaaaatgtat gtgtttcatc tcttgatgag gtaattatat ggcatcttgt acataaaaga
    29881 tatacaaaat cttacgcatg gggttaaaaa atatactatt ctgtacacat tttgttcatg
    29941 ttctgtacat aatttgtaac tattcagagc cgaaaagcat tacataattt cgtcgaacgc
    30001 ttgcattcgt aagaattatg taatgcttat tcggcgaagt aaccacatga gcaaaagaat
    30061 agctctgaaa gagcggtttt gcgagtgggg ttctgaatag ttactttctc tcttcaaaag
    30121 agaacaatat cttctggctc tcccgctcgt acatgttctc tctggtcaca atagtggtgg
    30181 ctgtgtctat cacgctgggg ccgccggtct gtccgttgat caggttcgcc gccgtctcca
    30241 ctcccagata ccccatggca taggggttct gcacgatcag ggcatccacc tccccggtct
    30301 ccaacattcc cacagagacc acattggagt caaacgccac cacctgcgtc ctgtctcccc
    30361 ggtccagatc ccggatggcc cagcccacac cgaggctggt ccattcgtta aaggtcacca
    30421 ccacattgat cctggaatcc gacaaaagca tctcttttgc tcccgctctg gcatcctccg
    30481 tggtggaaag gacattgatg gtggtaatat cctttactct cgggtcctgc tccaccgtct
    30541 gccggaatcc cagttcccgt tgctgcccgt tggcggaatt cacatcataa ttcacgatac
    30601 cgatatacag ttcgtcttct cccaccgcca gagctgcttc cgccgccttc acaccggcct
    30661 gcaggttatt ggtcccaatg cggcagctta ccatactgct gttgacatca ctgtcaatga
    30721 caacgatctt aattcctctc tgggctgcct gaccgatgat ctccgcattg gcattgtaat
    30781 ccaccgcaga gaaaacgatc acatccactc cgtcctccat ggcctgccgc accatctcat
    30841 tctgggtcac ataatcttct tccgattccg ggccgttaaa cgtaacattc agattgtatt
    30901 ccgtagccgc cgcccgggca cccgcaaaga cacttttaaa aaaggcgctc tccgtggact
    30961 tcacgatcat agccacctgg gttttccctg tctcagcttc ttttccgcag ccactcatgc
    31021 tcataagaag caggacaccc gcagccagca gggacagtat ttttttcatt ttctgttttc
    31081 tcatactttc tcccatcctg ccgctgcatt ttcttccacc ttcggcatcc gtatgatcac
    31141 agtagtgcct tcatccagtt cgctttctat ggaaagaccg tactcttttc cgaaataaat
    31201 cttaatcctg tcgttgacgt tcttgatgcc gatgccagaa ttatctcccg gctccttatg
    31261 aaggatcgac cgacactgtt cctctgacat accgacaccg ttatcctcca cccggaagac
    31321 cacatctttc ccttcgctgt agatgcggat cagaatccgt ccttcatcca ccatgcggtc
    31381 cagtccgtga tagatggcat tttcgataat gggctgcagg gtgatcttgt tacagtaata
    31441 ttgcagacag tcctcttcca cctgaaactc ataggaaaac tgattcttat agcggaaatt
    31501 ctggatcttt aaatagcttc tggcatgctc tacttctttt tcaatgggga tcaattcgtg
    31561 ccccttgctg atactgatac gaaacagttt cgccagagca ttgaccatct ccacggcatc
    31621 ctgacacctt tcttcctcgc agagccaccc gatggcatcc aacgtattat ataagaagtg
    31681 gggattgatc tgggcctgca gagcctttaa ctccgttttg cgcagggaaa tctcttcctg
    31741 acgtacctgc ttcatcagtt tctggatctt tcttaccatg tgctcgtagg actgtgacag
    31801 tgcctcgatc tccgtggtgc cttccacagg ctgataaaca tatcccgcag catccttttc
    31861 gaactcttcc atagcccgga tcaggcgctg caccggattg gtcaccagtc tggataccag
    31921 aacactgacc accatggtcg tcagcagaat gattgtcagc atgacacaga tgtttcccac
    31981 cagttccatc tctttggccg tcaccagttc actgatatag ctgacaccca ccaccttcca
    32041 gttacagtta tccagcgcca ggacactaca aatcagttct tcggtctcaa aggaaccgtt
    32101 gtggctcaga tccagtccct ccgtggcttc cgtcttaaga cccgaataca gcagctgctg
    32161 ttggggatga tagatgatct ctccattact gtctgtgata aagcagtagc catgctgtcc
    32221 gattcccaca tcgtccacat aatccgatat tttggagaaa cggatatcaa tgactacccg
    32281 cagcttctgt ccgtctgctc cgggaatctc ctgtaagacc gataccaccc agggataata
    32341 gtcctgcaac agagattcca catggggttc cgacagatag agtaccccgg ggctgtagga
    32401 atccagttcg atataagaca gatccttcag ataatgttcc ttcagagtat gagttcccgt
    32461 atgggaatcc accagcttcc cctgctcatc atagatatag accgccacca gatccgaccg
    32521 gacactgcaa agagtctcca gtgtctgccc ggtacttccg ccgctctttg tcagatcctt
    32581 ctcgatcaga ctcatgatct cctgcatatc tccgatgtag ttagaaatgg tattggaagc
    32641 ctgagtcacc gcctgttcac tgctggtatg tgccgcctgt ttcatacttc cgacataaag
    32701 agaaaaaaag taaaaagtgg accacaacaa aaacagggta accagtccca ccactaatgt
    32761 cgtaatgata aaagaaaggg acgttttttg cttcttcata tcttattctt tctcctgtcg
    32821 catggcattg ggagataccc cgcaatactt tttaaagcag tagctgaaat aatgctgatc
    32881 actgtaaccg cagcgctccg caatctcccg catcttcatg gaactgccca gtaccagttc
    32941 ccttgccgtt tcgatcctct ttttcgtcaa cagatccacg aagcttgacc cggtctcttt
    33001 tttgatcagg gcactcaggt aattggggct cacatgaatg ctgttgctga cgcccaccag
    33061 agacagggac ggatccccat actccttgtc gatgatctgc atggcctttt cacacaggat
    33121 ctggctgctc tttttcttgc ccgcattgac cagttcccat acttccaggc aaagtgccgt
    33181 gaaacgatcc ctggattccc gtatcggacc ttccagcatg gcaaaacgct ggagcagggc
    33241 ggaatcttct gtcaccgtat aggcaatgcg gatcagcgcc gaggacatcc ggaagatcag
    33301 gaatttgcat ttcagggcag catcctgctg cacctccagt ttgccgaata catccttcag
    33361 atagttttcc agttcctctc gggaaccacc acggatcaga ttctccatct gagtaacaca
    33421 ttccgccgcc agctccatgt ccatggtgtt atcccgctct tcatcggcaa tataatggat
    33481 gccgctctct cccggagtgc aatatcccaa tgcactcata gctcctctgt aggcctcgtg
    33541 gcattctccc agatgttctc tgatacggct gacaccgata tgacaatgca ttttcagaat
    33601 gcgttccgta ctctgtacca tctctcccac ggcaatatgc agataccggt cgaaggtcga
    33661 cgtggtggcc gccagcactg caatgatcct gccgtccatg gcaaaagaat aatgcttcat
    33721 atatttttgc agtatcatat ccacggaatg taccagttcc ggtctcgtac ggttcttccc
    33781 gttggtatcc tccaccgtga tggccagcac cgcatactga aaggcattat tacgatcctt
    33841 caaaaagccg ttgccaaccg cctgttcctg taaaagttcc tcccggtcct gcccctcaaa
    33901 gctggcataa tcatctagca aaaggggcag aaaaaactgt tgcagatccg gattatcatg
    33961 ttttctggca tcaaattccg caaagattcc gtcgatctta agcttgatct tcttcagttc
    34021 ttccgtcaga tccaccatag tgataggttt caacatataa ctgatgatat tgtactggat
    34081 ggcctgctgg gcataagaaa attcatcgaa gccgcttaag aaggcgatct gtgtggtagg
    34141 acgtacctct ctgacctgtc ttgccagctc gattccggag acaaaaggca tccggatatc
    34201 cgtaagcagc agatccggtt ccatcctctc caccagttcc aaagcttcga tgccgttctc
    34261 cgcttccccc acgacttgaa atccgatgct ctcccagtcg atcttcctta tgattgctct
    34321 gcgtaattcc tcttcgtcat ctgccactac gattgtatac attgctgtcc ctctctggta
    34381 tgtattattt taaggtaagt atcttcccgg aaaacgcaat gggaagggat atttttctgg
    34441 tactgttttc gcctgcaaac agaatctggg caatgccgtc agccgatacg gacgcaatcc
    34501 tgccggttcc gtatttctga tgctgtactt cccgtccgga ctcacaggct gcgatgcagg
    34561 cctccacttc ccgtggcggc agcttctgca cctttttgtc cggtgtcttt agcagcgagc
    34621 ctctgatccc gacagccgta ccggtctttc ttacagcgtt taacggcttc gttttctcaa
    34681 acacttctcc tgaaaaagca gaggtcagat tcggtccaaa ggtaaataca tacagctgtt
    34741 ccttcgctct cgtcattccc acataataaa gccttctctc ttcctcatag gcattggcct
    34801 ccggggaatc cggtacacag cccctccccg gaatctggga agggatcact cccgccagca
    34861 tatccgccag atacactctt gtgtattcca gtcccttgct ggaatggatc gtggacagga
    34921 taaaattact cttcggatcc ccgtagccct ctcgcatcat ctcctgcagc ttctgtaatc
    34981 ttgcagggaa atcaaacagg ctgttctcct gatctcccag ggcctttaat atatccgcct
    35041 tgccactgtc caacccgtgg gtatccatga attcctgata tcccatataa ttcaggatcc
    35101 ggtagatagc ttttcctgct gactcatacc gcatattgtc cagatgggta gccagtgcct
    35161 tgcactgttt tctggtgtaa acggatacct catccatttc cgccaccgta tccaacagtg
    35221 tcagtcgtct ggtattggca tccaccgcct ccatggccgc ctttttgctg ataccggctc
    35281 ccattttgta atagatccga agaaatgcct ccccatccca tggatccaga gacaactgaa
    35341 taaaatcaca gatatcgttg accaccggat gggaaaagaa ggtcatatcc gaatttttca
    35401 cccggcagga caatccttta cgttccaaca gatcaatgat gggaagtgca ctttcgttat
    35461 tccggtataa tactgccgtc tcctgttcac agtcctgggc tactttcagc agataattgt
    35521 actgtccctg tctggatact gccttgatct ctgtaacgca gccaccacct tctctggccg
    35581 cggtcatctt cttcggatgt ctgtttttat tcttctggat cagagtatcc gccgcccgga
    35641 cgatctcctg cctgctgcgg taattggtct ccatgagaag gatccctgct cccgggtgtc
    35701 tgtcctcaaa ggagacaagt gcctgcgggt aagctgcccg gaagccgtag atactctggt
    35761 cttcatctcc caccataaac agattccggc tctgcgccgc cagcaggtcg atcatatcat
    35821 gctgtatttt cgaagtatcc tgcgcttcat ccacacagaa ataccgatac tgctccctga
    35881 aatgtgcaag tacctcagga tactgttcca gaattctgag ggcgtagatc atctgatcat
    35941 cgtaatcgat catctgcatc tgcctgagtt tcgcattata agtacggaat atctccggga
    36001 atttctccac atccgtgtcc atatcccgga ttttctccgg tgtcagacgc atatttttcg
    36061 cataggtgat atccagctgg gtctctttga tatcattttc cgtggcgtat ttccccgtta
    36121 cttccagaaa agactgtttg acggccgccg aggcttcctt atctgccacc tgaaaaggcg
    36181 ttttcccggt acggtaggca aaatattgca gtaccttctg ggagatgccg ttgatggtgc
    36241 gaaactccag ccgctctgca tccgcttctc cgaacatccc cgcaaaccgc ctgcgcatat
    36301 cattcgttgc cgccaccgta taggtcactg tcaggatcga ctccggtgtt attcccaggc
    36361 aatagatcat atagcccagt ctgctgacca gtacggtggt cttaccgctt cctggaaccg
    36421 caagaagcag taccggacct tccgtagcct gtaccgcctg catctgctgt tcgtttaact
    36481 gtatgtgata gttccgtaca aattccgtaa aattcaattc attgtcccac tttccgcctc
    36541 cggcattttt ctgccttcct ttgtcgctat cggctcaccg tccagtactg catccagaag
    36601 gataccattc tcatcgtaag acagttttaa ggagaaaaag gggcggttct cacgaagcag
    36661 ccggaaaaat atcttgtccc cttcccagag atccagttcc tccaccttat tgatctccac
    36721 ccattccagc gctccttcgt cacactccct cggttccccc gtaaatccat ccgccgtata
    36781 caggcacata tactcacaga cgtcccggta taaaaaggtg atgattcccc ggaaccggta
    36841 ggccgtcaga gtataccctg tctcttcctt tacttcccgg agcacacagt cctccgggct
    36901 ttcgtccgcc tcgaaatgac cacccacgcc gatccatttg tctttgttaa tgtcattttc
    36961 cttcttcacc cggtgaagca tcagatattt tccgtcctgc tcgatgtagc agagcgtggt
    37021 cagatttctg agctgctgta tactcatttt ctttctcctc tatatcccta tcctctgaaa
    37081 aatctgccca aaattattat ttcttacaat gttccgcaat ggctgcgatc gctttctcca
    37141 tctggaacgg ttccgccaga tgtgcattca ttccggcggt cagacatttc ttcgcatctt
    37201 cttcatacgt cagcgcattt tccctattcc tcacattaca aatccatttt tgcatttgaa
    37261 tgcatattta atttatttta ccatattgac tgccggacgg caaccttatt gctccagcat
    37321 attctcaata aaaatcccat atcctctggt actgtcctgc acgagaacat gggtgacggc
    37381 ccctacgaat ttaccgttct ggatgatagg tgcaccgctc attccctgga cgattcctcc
    37441 ggtcagggcg atcaggtcag aatctgtgac tttcagttcg attccccggt tcacgttgtc
    37501 atgatcaaga tgaatgtcgg tgatctcgat atcatagtat ctggtggtgc catctacggt
    37561 acacaaaatc tgcgccggtc cctttcggat ctcctgtttt aatccaatgg gcatcgcctc
    37621 ttccgttccc attgccagtg ctttgtcatt gcacttgccg aagattcccc ggacactgtt
    37681 gccggtaata tcccccagaa tcctgtcatc agaatagacg atcattcccg tcatttcccc
    37741 cggatgcccg gccgttccct tctgaatgga gatgatctcc gtctggtaca gggttccgtc
    37801 attcatttcc atcaggttgc tggtgtccac atcattgatt ccatgcccca gtgctccgaa
    37861 atttccctga ctgtcaatgt aggtcatggt tcccacaccc tgggcattgt ccctgaccca
    37921 gacaccaatc ttatatttct tcgtctgatc ctgtaccgga tcgattttaa catcaaaata
    37981 ttcttcgccc cgccggatcg tcaaaatcgc tgcttctcca ttaccgtttt cgatccgggt
    38041 gatcagatca tccttgtccg ccacttccgt gccgttcagt ttcaccacat aatcgccgct
    38101 tttcaaaata tgttccgccg gagccacctt ctccccgttg acagaacgga actctccggt
    38161 acctatgacc attacccctt cggtcttgac atagattccg atgggaacac ctaccgggat
    38221 cagctgctga tcctcgatca cccggatccc cacctgctta aaaggcagga tcccgaacag
    38281 cttcacctgc atctcaaggc tgtccgtcat tccggtcttc atagtcaccg gctgactcag
    38341 atccagtgtc acggcacctt ggggaatatt actggttccc tgttcactga cacttaaaat
    38401 ctcacctttt gcgggaatat ccagtgccag tgattcctcc gattccgccc gcatataaat
    38461 aacagaagga atactgctgt ccaccagata atagctgtag ccaaagagtc ccataccggc
    38521 caggataagg gcagcacaaa ggcaggcccg gtaaatccgc agtctgcgca ccttctgttt
    38581 ccagagctgc aaaatgatga ctgcatcttc tcctgcctgc ctttttactt tttccgtttc
    38641 cgtaatccat agcttcatac acattccccc actgaacttc tgtcattgct gtatagggta
    38701 tagtgtacgt cacattccta tttttaatct gtgatcaccg ggatttgcgg tttacagttt
    38761 tctccagttt aaccacaaat gaccacattc ccactgcttt tatattcctc tgggcagtcg
    38821 tccgtcagaa tcgttccgct ctgaaacgcc ccgaaggtca cagagctgac atttccgaac
    38881 ttagaatgat ctgccagaat aaaacattct ctgcattgtc ccatggcagt ctttttcacc
    38941 atggcctcat tcatatccgg tgtggtcagg ccactctgcc tgctgatccc gttggttcca
    39001 aaaaagcctt tggtaaaatg atagctctgt aaattcatca gtgcctgatt gcctaccaca
    39061 gcctccgtgg agccttttaa ttcaccgccg accagataca cgcggaatcc tgccgctgcc
    39121 agtgtcttgg catgcgctac cgcattggtc acaaaggtgg cacttttctc tgtcagataa
    39181 gaaagcatac ttgctgtggt cgtacctgca tccagataga taaaatcctc cggctggatc
    39241 agtgctgccg ctttggcagc caccagctgt ttggcttcca cattgacttc tgccttctgt
    39301 tccaccgtgg gttcatgtgt tatcaccgtc cggtcacatg ccacagcacc tccaaagacc
    39361 tttaccagct tcccggcatt atgcaatgct gtgatgtccc ggcgtaccgt ggattccgac
    39421 gtatttaaca gatcttttac ctcggccacc gtaatgctgt ttttttcttc caataatttc
    39481 agaatgatct cataacgttg ttctgtcagc ataaattgtt attttctctt tcctttgtca
    39541 catctgtctc cagtatgatc ttacgcacgg gaagaatact tccgggagac atggataatt
    39601 catcaacacc catagccaga aaacgtctgg tcaacgatgt atctgcaccc agttctccgc
    39661 agatacctgc ccagattccg gccttatgcg cattttccac gaccatctgt atcatacgca
    39721 gcactgcggg atgatgcgga tcataaaatg catccagctt cggattctgc ctgtcgatgg
    39781 ccagtgtgta ctgggtcaga tcgttagtac cgatactgaa gaaatccact tccttcgcca
    39841 gctctgcact catcatgaca gcagccggtg tctcgatcat aataccctgc ttcggttctc
    39901 cgaaagccac accctgttct gtcagttcct gttttacctc cgccacaatt tctttgatcc
    39961 ggtggacttc ttccacgctg atgatcatgg gatacataat ggagatatta ccaaatgcgc
    40021 tggcgcggaa cagtgcacgg agctgtgtct tgaagatctc cggtctggtc agacagatac
    40081 ggatggcgcg gcatcccatg gccggatttt cctccggctc catttcgaag taatcgcact
    40141 tcttgtctgc accgatgtcc agtgtacgga tgatcacagg ctttcccgcc atggtctccg
    40201 ccacagtgcg gtagatctga aactgttctt cctcggtagg gaaggtttct ctctccagat
    40261 ataaaaactc actgcggaac aggccgatac cgccggcatc gttctgcaat acggccgcca
    40321 gatccttgct gttacctata ttagcataga gcatgatctt cttaccgtca atagtgatat
    40381 tttcacgtcc ctttaaggtc tggagcagtt ctttcttctc ctgctcctcc tgctttagct
    40441 gctgcatttt cgacagagta tcttcatcgg gatccacata gatgcagccg tttcgtccgt
    40501 ccacgatacc aagttttccg tctatggcat cggtcagagg gatatcggtg ccgatcagtg
    40561 ccggaatgct catggtgcgg gccaggatcg ccgtatggga attcacggat ccatgtaccg
    40621 ttacaaagga taataccaga tccttgttca actgtaccgt ctcggagggt gccaggtcgt
    40681 ctgccacaat gatcacgggt tcttccgatg ccgccatgcc tgccgttctg ccacccagca
    40741 cgcttaatac gcgctcggaa atgtctttga catccgcaga tctggctctc atgtagtcat
    40801 cctccatgga ggcaaacatc tgtgcaaaat tgtctccggt ggaagccacc gcatactctg
    40861 cattcacctg ctgcatatga atgatgttct caatggattc attgtaatca tcatcctcca
    40921 gcatcatctg atgaatctcg aaaatagctg cattggcttc tcccacttct ttcaaggctt
    40981 tctggtacag attctgcagc tgctccatgg cggtttgtct ggcctgtctg taacgagcga
    41041 tctccaggtc gggattctcg atcttcacac gctttacctg ctgctcatcc ttcttatgaa
    41101 cactgatcct gccaatggca attccgccga atacactttt tccctggtag acttccatga
    41161 cttttctcct ctcctgacga taatattatg attacttaga ggtttgcttt gacaaattcc
    41221 gtaacagctg cataagctgc ttcctcatcc tcaccgtcga aggtcagggt gatctcgttg
    41281 ccggccttaa cagccagacc catgatgccg aagattctct tgcagtctcc ggtcttgccg
    41341 tccttggtca gagtgatggc actctggaat tcctttgttg cctttaccag ttctcctgct
    41401 gggcgagcat ggataccttc gggatctgtg ataacatatt taaattcttt cattttctta
    41461 tctccttttc tgatttttta tattacggta actattcagc acccacattc gcaaaaccgc
    41521 tctttcagag cttccctttt gctcatgtgg ttactttttt cttcaataca cccaacagca
    41581 gtgcggacac taccgtacct gccaccagtg ccaccagata catcactgcg ttacctacta
    41641 cagggaatac gaaaattcca ccgtgaggtg ccatcagtgt gcagtggaat gccatggaca
    41701 gggcgcctgc cagtgccgaa ccgatgatgc aggaaggaag cacatgcaga ggatcggatg
    41761 ctgcaaaagg aatcgcacct tcggtgatga acgccaggcc catgatgaag ttggtgggac
    41821 ctgcgtctct ctcttccttg gtgaatttat tcttgaataa cagggttgcc agtgcaatgg
    41881 cacagggagg agtcatacct ccgatcataa cagctgccat gatgtcgtag ttgcctgcgg
    41941 taattgctgc ggttccgaat acataagctg ccttgttgaa ggggccaccc atatcgatag
    42001 ccatcatgcc gcccaggatc agtcccagta cgatccgnnn nnnnnnnnnn nnnnnnnnnn
    42061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42121 nnnnnnnnnn nnnnnnnccg atgaagcccg ccagtaccgg aagcatcaga ccgaatgcca
    42181 cgccgccgat attcttgaac attgcggcta tgggagtaat cgtaccaaag ttacttctct
    42241 gatctgcggg aagtgcattg atatcaacgt tcagtccgtc gatcaggaat gcaattgcga
    42301 tcagaatacc tccgcctact acaaaaggaa gcatatggga tacgccgttc atcagctggg
    42361 tatagatctt atgagctgcg ctttccttac cggttacggc tgtagttgtt tcgctgcttt
    42421 ccccgtggaa aacttccacg tttccactca ttgcctgttt taccagctgg tctgccttgc
    42481 caataccgtc ggatacctga cagctgatga ctttcttgcc gttaaagcgg tccatgggta
    42541 ctttggcatc agccgccacg atgatgcagt ccgccgcttc aatctcttct gcagtcagta
    42601 cattttttgc gcctgcggag cctctggtct caatcttaat ctgacagtct gctgctctgg
    42661 ctgccttttc caatgcttct gctgccatat aggtatgagc gattccggtc ggacaggagg
    42721 tcactgccaa cagcttaaag cctttcttct tctcggtggt aataccggta tcacttaatc
    42781 tgtcatcaat gctcttggca ctttcatctg ctgcatcaat gatctgtaaa aactcatcta
    42841 cgctttttgc gtttctcagg gaagtggtaa aattttcatc catcagcatc acggacagtt
    42901 tgctcagcac atccagatgt acattatcct ttgtattcgg tgctgcaatc aggaacagta
    42961 aggtcaccgg ttccccgtcc agagattcat actccacacc gtttttgatc accatggctg
    43021 ccagtccggg ggcttttacc gcatcacatt ttccgtgagg gatcgcaatt ccctctccga
    43081 ttcccgtggt actctcttcc tctcttgcat agacctgttc acgatacttc tccacatcag
    43141 acagctttcc gctttttgcc atcagttcca ctgccagatc cagtgtttcc tttttgtcag
    43201 ccggtgccgc atttagggaa atactatttt tgtccaaaag atctgtaatt ctcatgttgt
    43261 gacccatcct ctctttttat atttatcatt gttattgttc tacctgctgt aatacctgca
    43321 agacttcttc tctggtggcc agattctcgg aaaatgcact ggcgcttcct gcagcaattc
    43381 ccatatggaa cgcatgtaca taatctttct tttccatcca tccnnnnnnn nnnnnnnnnn
    43441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43501 nnnnnnnnnn nnnnnnnnnn nnnatgtcat ccggcatggt ataaggaata cttcctgcca
    43561 ataccaggac atctccgtct cccagtctgt caatcttttc catcagctgt tccaaagctt
    43621 ttgcatcaat atccgggcca cagccattga tctctgttcc gtcaatactt ttcagcttca
    43681 gattgattct ggtcattccg tgatcgattt tgatgaaatc agcatttacg ccaaactcgt
    43741 tcaatctccg gatcagttcc tctccagtga atcccgccgc atatcccagg gctgtggaat
    43801 ccagtcccag attcttcagt acgatagaga cattgattcc ctttcctccc ggaagaatca
    43861 gctctgatga ggttctgttc gtcattccca gctgaaagtc atccacggat acgatgtaat
    43921 ccagtgacgg attgaaagtc acggtacaaa tcatttctat ccctcctgtc tgtcatcttt
    43981 cttgttctta tgatgttatt atatcttcac attcagtcaa attcaatcaa tttaatccac
    44041 aaatatggtg ctttattttg gttgaatttg cacaaaataa aaggatgaaa tacttcttca
    44101 tcctttttca atgtttcttt cactatagca tttcccttat ctggcttctt taaacccgtt
    44161 tgcctgttct ttcatttcct ttgcattgga aagcgccgct tccgtcagtg catcactgcc
    44221 taacagtctt gccagctccg ccagactttc ctcttctgcc accttgtgaa tgtccgtaat
    44281 ggtcatatca tcggtactgc tcttttcaat aatgaaatgc acatccgcca tggctgcgat
    44341 ctgaggcaga tgagtgatac agatgacctg atgggctcct gccagaatac tcagctgctc
    44401 cgctactttc catgcggttc tgccgctgat accggtatcg atctcatcaa agatcaatgt
    44461 atcaatggca tctttccctg ccagtaccgt tttgatcgcc agcatcaccc gggacagttc
    44521 accgccactg gccacctggt tcaaaggttt cagactttct ccgggattgg tggaaatcag
    44581 gaattccaca tcatcatagc cttttgttgt aatggcctga ccgggtctga ctgcgatgtc
    44641 aaattccacc gtcaggaaat tgagacctat cagcgcatct ttcaatttct gcgtcagtac
    44701 cgctgcgttt tttctgcgga tctcagacag ttcttcacag gctttcttca gtaacgcctg
    44761 cttttcctcc cactcggcgt tcaatttctg catataggca tcataatccg ccagtttttc
    44821 cagataagct tctttttcct ctttgtaccg caggatctct tcgatcgtgt tgccgtattt
    44881 tcccttgagg tgattgatgg tattcagacg atcctctgta ctgcgaaaat cctcttcatc
    44941 aaattccaga tccgacatat attcggagac atcatgattg taatctgaca aaagactttc
    45001 cacctctgcc aactgttcct ccatctgttc cagtgcagga tcatacatgg tcacactacg
    45061 caatgcccgc agtgccctgc tcaaagaatt cccggcaccg tcttccgcgt cattgccact
    45121 gagttgatag ctctccgcca ggctttccgc aatttttcgg ctgttggaca ttttgcggta
    45181 cgcctgttcc agttcctcat cttctccggg ctgcagggaa gcatccgcga tctccttttg
    45241 ttcaaattcc gccagagact gttctctgct tctggaggta tcatccagag tttcctctgt
    45301 gatctttttc tccagggcag tacactcatg ataaagtgca gccacccgcc ctgcgcattc
    45361 tgcaaattcc gctcccgcat aggcatccag gatctccata tgttttttct tgtttagcag
    45421 agactggtgc tcatgttgtc cgtgaatatc gatcagcagc tccgaaagtt ccttcacctg
    45481 ctttgccgtc acggtctctc cattgatgcg gcagatgctt ttcccggagg aaagttttcg
    45541 gctcaggatc acggttccgt cttccgaagg ttccagatcc atgctcttta atttttccgc
    45601 cagcctttct tcctcgcagt cgaagaccag ttccaccaga ccgttctctt ctccccgtct
    45661 gagcatgtct ttctcgaact ttcctcccag tgccagattc actgatccaa tgagtaatga
    45721 tttaccggca ccggtctcac cggtcagtat attcagtcct tctccgaact ctacctctgt
    45781 ctcccggata agagccagat ttttcacgcg taaactacga agcatgtatt tccccctgtc
    45841 ttacctttct atcatcagat ggatcttgtc cataaggatc tgggtatctt ccacggtccg
    45901 cactgccacc atgatcgtat catctccagc gatacatccc accacttcgg agaatttcag
    45961 ggcatccagt gccgccgcca ctgccatggc catgccggat acggtcttga tcaccagaat
    46021 attctgtgcc atgtccatgg agataaaacc gtctctgagg acgcggatgt atttatctcc
    46081 catatgactg tcatcctgtt ttaaaatgac gtatttctgt ttgccgccac cggtgggtac
    46141 tttggacagc ttcagttctc tgatatctct ggatacggta gcctgtgtca cagcaaaacc
    46201 ggcttccttc agatagcccg ccagttcctc ctgggtctcc acatctcttt tttctataat
    46261 ctccacgatc ttgcgatgtc ttgttttctt catcttgacc tcatgccttt ctcacaacaa
    46321 ttcgtcagga atctctcatt ttccgatgca atacatccag gaagcttacc tgattcagct
    46381 gaataaactc ggtgatcttc tccgactgca caatacggat ccggtctccg gtacacatgg
    46441 tctctttgtg gctgccgtca taattggctt ccacggtctg gcttttgccc tctctgccac
    46501 cgggaatctc tataacgatc tcatcttccg gggacaggat aatgcttctc tggttcaggc
    46561 tgtgggggca gatcggtgtc agggtgatca gtttcgcctt gggctccacc agcggcccgc
    46621 ccgccgacag attatatccc gtggatcctg tcggtgtagt cacgatcatt ccatctgcct
    46681 tgtaatgatt cagaagctgt ccgttgacat agatgttgaa ttctatgatc tgcagggatc
    46741 cgcttcggga aatgacgatg tcattcaaag cccagccttc cgccacggtt ccatccgcac
    46801 agcagacttt accgtttaac atcattctgc gttctttctt aaaatcatcc cggatcaggc
    46861 ggtccaggga ttcctctaat ccagacagtt cgatctccgt catataacct acggttccca
    46921 gattcactcc gatgatgggg acaaggatct tcttcgtctc tctggctgcc tgcagcaccg
    46981 tgccgtcgcc gcccaatacg atcatgcagt ccacatctaa cggaatgtcc gttgcttcct
    47041 cttccgccac ggaatccgct tcttccttcc agtcggattc cttgaccttt aatttcaccg
    47101 tacagccttt ggtttccaga taattctgga tccgtttcgt ggtcgcaagc tcctgatcct
    47161 tatgcttgtt ggtatatatc agaaaatgct tcatagcttc gcactccttg tctgttacag
    47221 ggtatgagag ttgtctacga tacctttgat tacggcatcc cattcagggg tataggacag
    47281 tccggtcttt tcctctataa tagtctgtaa ggcgctctcc gcttcatgct cggtcagcgc
    47341 cagcttttct tcggagatct cctccggctt acggatatgc agcagatatt cgatattgcc
    47401 ctccggtccc ttgatggggg aatattccag atgcagtact tcgaaaccaa tgctgtccgc
    47461 atagtcgatg atcttgcgga tcacttcctc gtggatctga ggctctctca ccacaccgtt
    47521 cttgcccacc ttgcctctgc cggcctcgaa ctggggcttg atcagacaca ccatctgtcc
    47581 gccggtcttt aacagatttc tcgccgggat caaaatcttg gtcagagaaa taaaggacac
    47641 atccacgctg gcgaaatcca gtgcatccgg gatatcctcc ggggtcacat aacggaaatt
    47701 ggtctgttcc atgcatacca cccgctcatc attccgcagt ttccaggcta actgcccctg
    47761 tcccacgtcc acggaataca ccttcgtggc tccgttctgg agcatgcagt ccgtgaagcc
    47821 tccggtagat gcaccgatat ccatgcagat catgccgtcc agcagaaatc cccactgttc
    47881 cattgccttt tccagcttca agcctccgcg gctgacatat ttcatggtat gatttctgac
    47941 ttccagcttg atcttgctga catcaaatcc ggtgccggcc ttgtcttcct tctgtccgtt
    48001 gacatagaca ttgccggaca ttatgatggc ttttgccttt tctctggaag gagcataccc
    48061 ctgctctacc agaatcacat ccagtcgttc tttcatcttc gtctcctaat tatttataat
    48121 ctttccagaa ttttctccgt gatgctttcc gcatccaatc cgatctcctt tttcaaaagc
    48181 tctacatttc catgttccac ataagcatcg ggaatagctg ccatgacaca tttgatccgt
    48241 ttctcgtggt cattcagaca atccaacacc ttttctccga atcctccgct ttggacattt
    48301 tcttccatgg tcacgatcat ctcatgatct tcacaggccg aaagtactgc ctcttcgtca
    48361 atgggcttga taaatctggc attgatgata cttgccttgt aacccttttc atgcagaagc
    48421 ttctggactt cctccgctgt ctttaccata ctgcctacgg caaacaatgc gataccgccc
    48481 tctcttgcga tccactccgc cttgcccagt tcaatggggg cacggtgttc ggcaagtccg
    48541 gcataagccg ttcctctggg atatcgcacc gcaatgggcg catccagtgc cacggcaaac
    48601 ttcatcatat ccgataattc ccatttgttc ttcggcgcac agatatgcat attgggaatt
    48661 cccgacagat aggtataatc aaaaataccc tgatgggtct caccgtcact ccccaccagc
    48721 cctgcacgat ctatagcaaa cactaccggc aggttctgaa tacacacatc atgcaggatc
    48781 tgatcgtagg ctctctgtaa aaatgaagaa tacaccgcaa caataggcct catgcctcct
    48841 gctgccagtc ctgctgcgaa ggtcaccgca tgttcctccg caattcccac atcaaagaac
    48901 cggtccgggt acatattccg gaagcgcttt aagccggtgc cgtccggcat ggctgccgta
    48961 attgccacta cccgttcgtc ccgctggccc agcttgcaca taacagtgga aaacacatcc
    49021 gtataattgg ccacggtgcg gggatgggaa ggaattcccg tctcgatgtc aaaaggctct
    49081 gccccgtgaa atctggcggg atgtttctcc gccggtccat agcccttgcc cttctgggta
    49141 atcacatgca ccagcacagc tcccttgact ctcttggctt cctcaatgac cttgatcagg
    49201 ccttcgatat catgtccgtc taccggtcct aaataagtca ctcccatatc ctcaaaaaac
    49261 atcccgggga tcaccagctg tttgaagctg ctcttggccc ggcggatact attgacaata
    49321 ccgtcgccac cgggttttct cttgagagcg ttgtatatgc cctctttcag atccagataa
    49381 ccggtcgccg tacggatatt gttcagatat ttggaaacac cgcccacatt ttcggagatg
    49441 gacatattgt tgtcgtttaa taccacaata aagttcgtct ccagtttggc tgcattgttc
    49501 aaggcctcgt aagccatgcc tcctgtcaga gagccgtctc cgatcacgga tacaatggtg
    49561 gtcttctccc ctttgagatc tcttgccttc accagtccca gtcctgcgga tatggaggta
    49621 gaactgtgtc cggtatcaaa tgcatcataa tcactttctt ttctcttggg gaacccactc
    49681 atgccatgga actggcgtaa gacatcgaaa ccgtccttgc ggcccgttaa tattttgtgg
    49741 gtataagact gatgacctac atcccagatg atcttgtcct ccgggagatt cagagcaaga
    49801 tgaagtgcca tggtcagttc caccgctccc aggttcgaac ccagatgccc cccggtcaca
    49861 ctgatctttt cgatcaggaa ctgccgaatc tcctccgcca gttccccata gtctgattta
    49921 tctatttttt tgatatcgtt tgctttctcg atcttatcca gtaacattgg cttttcctct
    49981 gctatttcgt tctatggacc aggtgcagca acagctcctt caggaattca tgttctcccg
    50041 aaaagctgtc cagagtcgct accgcttccc cggtcagccg ttccacttct ctgccggatt
    50101 cttccagtcc gtgtaaagtc acataggtct gcttatgatt ctgtgcatca cttccggtag
    50161 cttttcccag ctccttgctg tcccctatca catccagaat gtcatcctgg atctggaatg
    50221 caatgccgac attgtaggca cacagttcca atgccgcaat ctcttcatcg gttgcccctg
    50281 ccagcgtagc tccgatagtc atggccgact ggatcagggc tgccgtcttg ttggcatgga
    50341 tgaacaggat ctgttctgtg gtcagaggtt ctgcctgttc ctccgcttca atatctgcgt
    50401 tctggccgcc gatcatccca tagattcctg ccttgcgggc taaaatattc agtgccgccg
    50461 ccactctcgg gtaattcgga tctaccggtt ctcccgaggt gcatgccgga aaagcacgaa
    50521 atgcagtctc aaaggcataa ttgagcagtc cgtctcctgc caggactccc ataccgtcac
    50581 cgtacacaga ccaggtggtc ttgcggcctc tgcggtactc gtcattgtcc atggccggca
    50641 gatcatcatg taccagagaa tatgtatgga tcatctcgat cgccgccata aaaggctcta
    50701 tcgtatcatc tttgccaccg aacattttat aggtctcctg catcagcatg ggacgcaggc
    50761 gctttccgcc tgccataaga ctatagttca tggcttcaat aacggtcttc tggtatcctt
    50821 cctctgccgg aagatattcc ttcagtaatt cttcaatcgc tgccgttctc ttctgcaatt
    50881 cctgtctgaa taacgtttct tccatgtctg atgccaacct tcctctacct gccggtatgc
    50941 tgtatgctta ctggggatcc agcggttcca gtccgccctc ttcgttcagc ttcatgacct
    51001 gtttttccac ccggtcgatc tgttcgctgc accgtttcag cagattcatg cccttgctgt
    51061 acgccgtgaa agcttcctcc agagaaatat cctctgcttc cagctgtccg atcagttctt
    51121 ccactgcgga aaaattctct tccaggctct gtggttcctg tgcttccgtt ttcttttttc
    51181 ctgccaaaat agcacctccg tctgctgcct gcatctatct gcatcccccg gataccggta
    51241 aatgctatcc gggaagtctt tcttccttct gtatctgttc cacccgggta tcgatcacgc
    51301 cgtcactcac ccggatccgc aggcgctccc ctgcttctac ctgggtcacg gaacggatat
    51361 ttttcccatc ggtatcctcc acataggaaa aaccactgtt taacttttcc aaaggagaga
    51421 ccgctttcat tctctcaata tacaatgcca ggttatgtct gcgttcccgc aggatcgcct
    51481 gcagccggtt ctgcagccgg tcttccagct gtatgctgta agtcctcttc tcccgtatcc
    51541 ggctggcggg gcttaaatat ttcaggcgca tttccatctg atccgcctgc tgcctcttat
    51601 gctgaagcac tcgtcccatc cgctgataca gcctgtcctg tctgtccaaa atctcattct
    51661 caatatcact gacctgtgtc actgccagtt ctgccgctgc cgacggggtc ggtgcccgca
    51721 gatccgccac ataatcaatg atcgtggtat ccgtctcgtg tccaaccgcc gaaataatgg
    51781 gaacagagca gtcgaatact gcctgtgcca ccattctctc gttgaacgcc cacagatcct
    51841 caatggagcc gcccccacgt cccacgatca tgacatccac gccctgtctc tccagtgcac
    51901 ggattccgtt cacaatggaa ggcgccgccg aagctccctg taccagcgca ggatacaaaa
    51961 taatctgcac gaagggattc cgtctggagg cgatctgtat gatatcccgg actgccgcgc
    52021 cggtatctgc cgtgactacc ccaagagtat ggatatactt cggaatctgc tgcttgtact
    52081 ccggagcaaa catgccctct tccgccagtt cccgttttag ttgttcgtat ttttcataga
    52141 gggctccggc gccgtccagc gtcacttcct tcacatacag ctgatatctg ccgtcacgct
    52201 caaatacact cacactgccc ttcgcaatta cctgctgccc ttccgtcagc cggaatccca
    52261 gacctctccg gtctccggcg aacataatgc agttcagggc actggcagaa tcctttaacg
    52321 taaaataaat atgtccagag ggatggtatt tacagttgct tacctcaccc tttacagata
    52381 ccatctgtaa aaagtaatcc tgcgtgaaca tatttttgat ataagtattc agttgtcgta
    52441 cgctgattac gttctgtatc ctactcaccg gatttcatga tcttggcaag gatcgcattg
    52501 acgaatccgc cagagctttc ctgtccgtac tttttggcaa tctcgattgc ctcattaata
    52561 gcaacgccat tcgggatatc gtcatcatag cgcagctcat atgcaccaag gcgcagtact
    52621 gcaagctcca ccttgcccat acgggtggta tcccagcctt cggtattctc attcaacagc
    52681 ctgtcaagct cggggatttt ctccctgatc ttctcaaaac gggccgcaat ggcatctgca
    52741 tcctgccagg aaggaacttc ctcattattc tcgcggaaca atgcgatctg ttccggcata
    52801 tcctccggtg catgaaactc tacgcggtat aaaagtttga aaacctgttc tctctgctcg
    52861 tgtcttccca taatatcttc ctgtaattcc tcttatttat ccttcttcat gttcacaccg
    52921 gcaatacgga tgttcacatc agagcaggtc agtcctgtca tattctcaat ggcatttttc
    52981 accttctgct gtactgcctg acaggtagcg ggaatattgt aaccgtactc catggttaca
    53041 gtcagatcca cggtcacatt cttgtccagc acctctactt tgacaccctt ggtcaggctc
    53101 ttaacgccta ccttgctcat gagttcgttg gtaacattac cagccatggc gctgacaccg
    53161 tctacctcgg tagctgctaa agatgcgatc atagccacta catcatcggc gatctgtacg
    53221 gtaccgatgc tctcgtcttc ctttaatacc aggttcttct tatctatttc tttttccatg
    53281 atagctgatc ctcctaataa taactaatta gttacagtat accaaatcct tattcatttg
    53341 tcacccaaaa agtcaaggat aattgtttcc cgttctgggc ataagtgacg gtattgctgt
    53401 tcccctgtat ctgcatatag gaaaaaatct cctgctcatc gatcaatgta tgacagattt
    53461 cctgataaca gagttcatct gcacatttta tcgtcacttc cgttctgccg ccctctatgg
    53521 ctctgtcgaa gaccgcctgc atctgttcat ggtcatagga agtaaagtag gaaccttccc
    53581 gcacataata atttgcttcc gtcgccgtac attccggcat ggggatcacc gcttcgatac
    53641 gatgcgtccg tagcagatcc tctgtagtca cgcacagata atcataattg atctccggca
    53701 ggctcctctg ctcgctgcca ttctccatgc ggtaagaggc atctccccag gtggtatcta
    53761 cataataata gtccccgtcc acctgtacca gattccaggc atgagcttct ccggtatcca
    53821 ccgttccctg caccagggtg cacatcaccc ccagatgatt caacagatac tgggtggctt
    53881 tggcatatcc ctggcagaca gaggctcctc ctaagaatac cgaggcaatg ttctgattat
    53941 ccgccgcact cagatcataa tttgtggaaa agatgaccgc ctcatacacc tcttttacct
    54001 tatcgtactc agaagccgcc tcttccatac cggacagcca ttccgccgtc acactgcgaa
    54061 tggcctccgc ccgcgctacg gcctcttcct tcgtctgatc gtaagtgcct gcgaagtcaa
    54121 ttcctacggt tttctcccct ctggaatatt tggtataagt atatcctgtg gtgtaaaaaa
    54181 tctccggatg atctatcagc acgcactgaa atactttgtc aatgtcctgt tcatccattc
    54241 cctgttccaa cggttccgtg gacagtttga tcgtgtcatc catagaccca atcgcctgct
    54301 cgatctcctg ataccagatc tgttcctgct catccagact ctcatatgca aaacgagagc
    54361 ctccctcctc taaaagggaa aagctctctg tattgtcatt ttccgcagcc gccgtctcct
    54421 gcacaggcat ggtatctatc tgtgactcgg gcagcacgat ggtctggggt acttcctgca
    54481 gaacctcctg tgaggagcat cctgtcaaaa gcagtaacat cagaattccg gcacataact
    54541 ttttcttatt ccgcattcta tccccctaca cactttttgt ttccatataa ttactaattg
    54601 cgtccgaact ccgtcagcac cagtccctta ttacggtcca gagtcatgcc gatgctccag
    54661 gaattcacaa acagcaataa aggattaccc ttcatgtcct tgaccttctt catatcggaa
    54721 gtaccggaca gcttggccag gtcgatctca tccttattct cgatccagcg gatatgctca
    54781 taaggcaggt tctccagacg gatctccacg ttatattcgt tctccagacg gtatttcagt
    54841 acatcgaact gcagcacacc taccacaccg acgatgatct cttccagacc ggtattgaac
    54901 tcctgaaaga tctggatggc accttcctga gcgatctgct ctacgccctt gacgaactgt
    54961 tttctcttca tggtatccac ctggcgcact ctggcaaaat gctccggtgc gaaggtcgga
    55021 ataccttcat actggaactt ctccctgggc atgcacagtg tatctccgat ggagaaaatg
    55081 ccgggatcaa agacaccgat aatatcaccc gcataagcct cgtttaagat cttacgttca
    55141 tccgccatga tctgctgggg ctgggataat ctcataacct tattgccctg cacatgacgc
    55201 acttccatgt tggcatcgaa tttaccggag cagatacgca tgaatgcgat acggtctctg
    55261 tgagccttgt tcatgttagc ctggattttg aaaacaaagg cactgaactc atgctccgtc
    55321 gggttgatca tgccgatgtc tgcttttctt ggcagcggcg tggtagtcat attcaggaaa
    55381 tgctttaaga agatctccac accgaagttg gtcaaagcag atccgaagca taccggggtc
    55441 aggttaccct tctgcacttc ctccagatca aactctgcgc tggcaccgtc caacagttcc
    55501 acctcgccaa gcagcttgtc cgctgcatcc tctccgatca gttccttcag atgttcttcg
    55561 tcctgaatgg gaatctcggt ggaagttcct tccttcgttc ccttctccgt atcggaatat
    55621 gcgatcacac actttttctc acggtcgtag acaccctgga attccttacc ggaaccgatg
    55681 ggccagttga tggggcaggt ggcaatgcca agctccttct cgatatcatc cagcagttca
    55741 aaggtatctc ttgcctcacg gtccatcttg ttgatgaagg tgaaaatagg gatcttgcgc
    55801 atcacgcaga ccttgaacag ctttctcgtc tgggcctcga cacccttgga agcatcgatg
    55861 accataactg cggagtccgc tgccatcagg gtacggtagg tatcctcaga gaaatcctga
    55921 tgtcccgggg tatccagaat attgatgcag taaccgtcat attcaaactg caatacggaa
    55981 gaggtaacgg aaatacctct gtctttctca atctgcatcc agtcggatac cgcatgtctg
    56041 gccgttgcct taccctttac gcttcctgcc aggttgatgg ctcctccgta gagcaggaac
    56101 ttctcagtca gggtcgtctt gccggcatcc gggtgggaaa taatggcaaa cgtccggcgg
    56161 cggttgatct cttctgtata attattactc actactatag tcctccaaat atatctctta
    56221 tatctctaaa aatctatttt cgcagttctt cgggatctgc tatctatgat ggatctgcct
    56281 ttaaaacaga gtttccttca aactccggtt ggattccgaa ataatccaat accgtagctc
    56341 cgatatcgga aaaagtcggt ctggttccgc gattccccgg cgtcacaggc gcaccgtaca
    56401 taagaaacgg agtatattct ctggtgtgat ccgtggtggc tgtgtaagcg ggatcacagc
    56461 cgtggtctgc ggtgatcatc agaatatcat ccggcttcat gtgtgcctgg atctccggca
    56521 gcttctcatc aaaataagcc agtgccttcg catagccgtc cacatctctt ctatggccgt
    56581 agagcatgtc gtaatccacc agattgataa agcagagtcc ttcgaaatcc ttgtccagat
    56641 attccagtgt ccgctcaatg ccttccgcat tgccggaagt gtatacatgc tcggtaatgc
    56701 ccttgcctac gaaaatatcc tggatcttac ccacagcaat gacatcctta ccggcagcct
    56761 tcagctgatc cagcatggtc actgcgggag gcagcaggga aaaatcgtgt cttctggggg
    56821 tacgggtata atgtccgctc tcaccgataa aaggtctcgc aatgacacgg cccacgccgt
    56881 gttctccctg taaaatctgt ctggcgatac ggcaataatc atataactgc tccacaggaa
    56941 ccacatcctc atgggcagcg atctgaaaca cactatctgc ggaagtatat acgatcagtt
    57001 tgccggtgcg cacatgctcg tccccgtaat ccgcaatgac ctgtgtgccg gagtacggct
    57061 tattgcacag cacatctctg ccggtgcgga tccggaactc ttccagcaca tcctcgggga
    57121 agccgtccgg ataggtaggc agtggcttgg gagaatagat acctgcgatc tcccagtgtc
    57181 cgatggtcgt gtcctttccg cgggaacgct ccgtcatgcg ggtataagct gccgtgggat
    57241 ggtctgtttt ctctcccact tccacgccgt caatattaaa caaacccatg tttttcatat
    57301 tttccatatg aaaaaaaggg ctctttgaca cgctccccag tgtatttaca ttgatgtcac
    57361 cgtaagctgc ggcatccggc atctgtccga taccgaagct gtccaataca attaaaaaga
    57421 ctctcttcat tgtttcttcc ttttgtcaag ttttccaggt taagtnnnnn nnnnnnnnnn
    57481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    57541 nnnnnnnnnn nnnnnnnnnn nnnnnattaa aaagactctc ttcattgttt cttccttttg
    57601 tcaagttttc caggttaagt catgatacca tccagattgt tccgtgcttc gcaccctcac
    57661 aatctggacc gcattcgtaa tccgctcatt tttccgcgaa aaatggctcc ttactcatgt
    57721 ggtacggctc tcgaatctcc ggatgctccc gcaagcgagc ttgcatcgca ccgtcgactc
    57781 tcgacccttg gtatcataac ataatttttc ccctatgtac acaaaaatat cactgtgcaa
    57841 tggtgctgat gacgatattt tccgccgcaa tatccgtctt tctttttacg atatcttcga
    57901 tctgtgcccg ctgcacatcc gtcagttctg cggcattgac caccacatcc acggcatccc
    57961 cgttgatgct gaccacagct tcactgaaac ctttcgcctg caaaagcacc tcggccgccg
    58021 cttccttttc cgcgatatca gtcatgcgca ccatgctgtc cactgcttcc tgtttctggg
    58081 catcgctgag ctcagtactg ttgataatct ccaataaggt ctctttattc cgggctctgg
    58141 tctgctcctt ggaaagtctt gcagcagaca gtacctccac accgttcgtt ccggtcaaca
    58201 ctgcttcccc gggaatctcc ccttcgtccg gcgtagcctc tgccacctgc atatcttcat
    58261 ccagataatc ttccatgacg atctccacat ccgaatccag gctttccata tctgtgacaa
    58321 tgtctgtgga atccactgca ctgagatctt ctgtattatc cgacgccaga tactcctttt
    58381 ccaggttggt ccccgcaaac tgcagataac ctgcaatggc tatcatgatg gcaagtgcgg
    58441 taatcataag ctgattcttc ttaattaagt ttttcactgc gttccccttt tccaaaggcc
    58501 tgtatctgcc ttcttgattt tatactctat tgtatgtacc cgtctgtgcg gatatgtttc
    58561 ctgtgactca tttttccaac accttgactt tgtgggcttc cacaccgaaa agtgcctgtg
    58621 cgatctgcgt gatttcactg gtcatattgc cttttccggc tccttccgcc accaccagca
    58681 ccccctccac tttgggtgtg atcgtcttcg ctacaaacgg tgtgccctga ctgccgtcct
    58741 gatagacggt ctggtatccg gtattggaag aactcacggt gcgggttcct ccggcggagt
    58801 cctgttcttc tgtctgggaa cgatccgcgg tcatgtcttt ttccacaatt ctttcctccg
    58861 aggattccaa tgtgatcatg acttccaccc ctcccagtcc ccggacggat tccagcattt
    58921 tcttcagttt tccctccaga taggcggtat attcctccgc gcttcccgct gccaccgccc
    58981 gggtttcggt atctaccgcc gtctcctgtt ccccggttgt ctgcgaagga gaagtgaaaa
    59041 ttccggcatt gtactgttct gcagtctctg tgccaacgga agctgcctgt ggctcctcct
    59101 ttttcaccgg cagggcgatg ataaacaata ggacacccaa caataccaga atcaacaggt
    59161 tgtcccgacg gaaccatttc ggagggccgg aaccgcctgc accgttacct gtaccaatgc
    59221 tgcctgtgat cttttcccaa aattcaccca ttgcttctca cctctttttc ttccctggct
    59281 ccggtttttc taccgtcatt ttccttaact ctccggcaga cttctccatc attccagccg
    59341 gattggtgca atgtccacat ggatcccctc cgtttctgca ttttccctat ctgtttcctt
    59401 ctgatccgat acttccccac tgttttctcc tgtaacagct ccctgtgcag aatcttgctc
    59461 ccacacattt gccgggtcat atccttcccc ggcttcctgc gccacagcag ctgccgtatt
    59521 cccaatctct tcctgcgact gttctgcctg ttcggtggcc cgggtcagca cggtctgcaa
    59581 ttcttgcata aatcctgtca cctgttcttc tgcattccag ccgtctccgc cgccgaacat
    59641 gctcagaaca ggctgtaaca actgcagcaa cagcataacg gataataaca gtttcaaata
    59701 tttatcgtag gactccttcg gtctgaaatg cactacggtc tgtgcacaga tcaggaagat
    59761 cccagcctgt ccgatcaccc ggtataacca ttgcatcctc ttacctcccc ctgcttccga
    59821 tagctaatag tgccaatgtg atcagaaaca acagcatggc tgtgcccgca gtacgaaaca
    59881 gaagcatccc tgcatttccc atatgatccg tacagttggt cactctttta tcgctgacga
    59941 ttcccataaa agctgccgca cacttgatca aaaacgcagt aaacaaaatc tccagaagcg
    60001 gagccgcaca cgccgccaga agcagtaaca gcaaaagtac gccgatgctg ttgcggatga
    60061 caacggctga acccaccgcc agctccagaa gcccgtctgc cgcatttccg atccccggca
    60121 gtgccgaaag tgccttttcc acggcggagg tcttcacgga atccaatacc ggtgtgatca
    60181 atgactggaa cacactgatc cccgtgacca ctcccagaga agccttgaga atccagccca
    60241 ccgccttttc cagaagttcc gtcaaaagtg tcagcttttc ctccacccag atcccgttga
    60301 tcatggtcaa caggccatag cagcaaatca gtggtagtac cacacccaac agtatttttt
    60361 ccgtaccata gatcagaagt agcatcagct gatacccggc tcccactgta gccggccccg
    60421 aggccactcc caccgcaaga agatacgtag gtaccatcag ccttacaaac aacacgatat
    60481 tttccaccgt ctctcttgcg gttcccagaa tcccggaaaa acattgcagc aggatcactc
    60541 ccatgagcag atagataaag taaaagctta aatccgcaat ctgatgtttg tcgaaaacct
    60601 ccacgaaatg aatcagcagt gcagaaagga tccccagtac gatcagccag atcagaatat
    60661 ttttcctgcc cgcagcagat gctcccattc cctgtatcac ttcctgcaac agggttcccg
    60721 ctgcccccag cacatctccc tgcagcagtt gtcccatcaa ttccgccacg gagatccgtg
    60781 aggccggaaa cagcttctgc atcccttttt ccagttcttc catgccatat tcctgccaca
    60841 ttgcatccat tgctccctca ctcttataaa ataccataga tctgtcggat caccgtaagc
    60901 agaatcggca gtcccgcaaa cagtaccgaa agttttccca gaatctcgat ctgatccgat
    60961 accgctgcga atccggcatc cttgcagatt ccggcgctga attcacacag gtaggtgatc
    61021 cccaccactt tcatcaaaat accgagatac ccttttgcat ccccgaggta ctgctgtaat
    61081 cccgcaaact cctccagcaa ctgtcccgtc agccccagca cataggaaaa taccagcaca
    61141 ctcagggcaa atccgatcag cagcgtatat tccggccgct gttgcctaaa ttgtatggca
    61201 aaaagaactc cggcgattcc aagcaatgca atcttaatga tctcggacat atttcctcct
    61261 attaaaacga aaacagagtc tgtacggtat caaacagatc atagatatac ggaacgatcc
    61321 agctaagaac caggatcagt cccgcaaggc tgacgagaaa agcatgttcc tccctgccgc
    61381 tgtgttttaa gacctggctg agtatcgtta caaggattcc taccgcagct attttaaaga
    61441 tcagtcctat tcccatcttt ccccgtcctt ttctcttctc tctgtggtct ccggaaccgg
    61501 catcagacta acaataacag tatcagcaga ccgctcatgg ctcccagtcc tacggagagc
    61561 ctgcatttct gtgtaatttc ccgctgctgc atatccgccg tatgttccag tccctgtctg
    61621 gacaattcga tactttttat ctgcatcgtt tcatccgcat agccctcttt ttccgcaaaa
    61681 cgcaaaaact cttcaatgtc ttcttttttc agaggaagca tccgcagctg tctgcgaaag
    61741 cttttctccg ccagttccag aaaaccagca tcctccgtct ccgcatattc acggtatact
    61801 tcggtgcaga tctctcccag cctgtctttc cgttgtctgc cggtctgtcg gcagcattcc
    61861 ggaagtgttg acccgttgta ccggatctca ctgatccact gttccagaat gcggatgagt
    61921 tcccggatcg tatataatct tcctaagaat tgctgccgat accacagtcc cattccaaag
    61981 catcccaaca gaatcagaaa tcctccgagc atctgcattc catccgtccc cttcctttta
    62041 agatcactat gcggtcaaat atctgtcgca ggcaggattc acccaggcgc tttgctgcgt
    62101 ccgtctcgtc cgttccatgc agagtggcca gcagaccgca gccgcaggcc gctgcttttt
    62161 gtaaggcccg cctgtccgtt tcctccccca gttcatccac tgcaatgact tgaggtgaca
    62221 tggaacgcaa aagtaataac atcccctgag ccttgggaca gccatcaagc acatccgtcc
    62281 gcatccccag atcattctga ggtcttccca gatagcttcc cgccagctcc gagcgctcgt
    62341 ccaccactcc cacccgaagt cccgcaccat acgtattacc atcggaaatc tggcggatca
    62401 gatccctgag cagagtggtt ttgccgaatc ccggaggcga tacaatcagg gtattctgaa
    62461 attttccttt cttatataat tttggcagga taccgtcagc tgctccccgg atctcatggg
    62521 cgacccggat attgataaac gcaatatttt tcagagtgcg aatcactgtt tcattttcca
    62581 acacagcctg tccgcacaat ccgatccggt gtccgccttc cacagtcaaa aagccctgtc
    62641 tgatctcatc ggcataagca tacaccgaat cctgacacag ccaggcgatc aactcccgta
    62701 tctccgcttc cgtcaccaga tgcgctccgt aaggatgctc ctgccattcc ccgccgttat
    62761 ttaagaaata atcttctctc gatgtttcca cgatcaccgg cctgtccaca cgtagacgga
    62821 tctcgcagat tttttcccca ttatccgctg tcttctgcca gaatgctctt ctcttttgcg
    62881 gaaaaatacg aagcaggctg tccatgtttc tcctcccgta actattcaga acctcatgcg
    62941 caaaatcgtt ccttcggaac ttctcctttg cgaatgcagg cattcatcaa gacttagctg
    63001 atatttaatg ctctgaatag ttacaatata tgtctccgtg gacaggatat tcctttcaat
    63061 cttctctttt tttctatagt gtggtataat aagcgataac tgtttttatg acttttgttt
    63121 tggaggtttt tatgaacata atcattgtgg gctgtggcaa ggttggctac accctggtag
    63181 agcaattagg cggtgagaat cacaatatca ccgtgatcga taccaatgag gaaaaggtga
    63241 attccatcac ggatgatctg gatgccatgg gcatcgtggg caatggtgtc agcttccaga
    63301 cactgtctga ggccggcatt gaccatgcgg atctgctgat cgccgtgacc ggatccgatg
    63361 agcagaatct tctgtgctgt gtcattgcga aaaaggcggg acattgtaag accatcgccc
    63421 gtgtccgcaa tcctatctac aataatgaga ttgcttttct gcgggaagaa ctggggcttg
    63481 ccatgatcat caacccggag ctgtccagtg ccgccgagat tgcccggatc ttccagtttc
    63541 cttctgcagt taagatcgat actttttcca agggaaggat cgagcttctg catttccgcg
    63601 tgacccatga ctgtctctta aataattatg aactgatcca tatccgtacc accttaaaat
    63661 gcgatgtact ggtatgtatg gtcaccagag ataatcaggt gctgattccc cggggtgatt
    63721 ttgtcttccg ggaaggcgat gtggtagcta ttgtgtccac tcctcccaag gccaatgatt
    63781 tcttcaaaaa gatcggtatc ggcgccggca gggcacgtac tgccatgatc gtcggtggcg
    63841 gaaccattgc ttattatctg gcaagaagac ttttagaagt cggcatccat accaagatca
    63901 tcgatcagga tcccgctcgc tgcgagcatc tgagtgaact gctgcccaag gcttccatta
    63961 tctgcggtga cggtacggat gaacgtcttc tgatacagga ggggctggag aacgtggatg
    64021 cttttgctgc actgaccgga ctggatgagg aaaatatcct cctgtcactg tatgcgaaac
    64081 ggacttcccg cgcaaaggta gtcaccaaga tcaaccgtat caacttcagt ggtgtcttaa
    64141 gtgatatcaa gctggatacg atctcctatc cgcgcctggt aactgcagat atgatcatta
    64201 aatatgcacg atccatgaat gaatctctca acagtaatgt ggagaatctg tataagctgg
    64261 aagacggtca tgcagaggca ctggaattct acatcaaaga ggattctgcc gttaccaata
    64321 tacctctgaa ccgtctgcac atccggaaaa atatcctggt ctgttccatc gtccgcggag
    64381 gacaggctat tattcccagt ggtcaggacg agcttcatgt aggcgattat gtggtcgtag
    64441 tactgacaca tgcacggctg aatgatatta aagatattat tgaagggtaa ttttgtagaa
    64501 ggacactatc atgaattttg cgatattgag ttttattatc ggatttattt tacaatttga
    64561 agctttattc ttactgcttc cctggatcgt gggtatgatc tacggagaat atcatgtggc
    64621 actgatctat ctggtgaccg cagctgtttg tttcatttta ggaaaattgt taagtttcag
    64681 gcacacagga cgttttaagg aattgtacgt cagggaaggc ttcactgccg ttgccctggg
    64741 atggtttgtc atgtctgttt tcggtgctat tcccttcgtt ctcaccggag agattccttt
    64801 ttatattgat gctctcttcg agacgatctc cggctttacg accacaggtt ccagtatcct
    64861 gtccgatgtg gaagcactca gctatgccag cctgttctgg cgcagcttca ctcactggat
    64921 cggcggtatg ggcgtatttg tatttattat ggcgatcctg ccgatgatgg gtggctccac
    64981 catgaacctg atgcgtgcgg agagccccgg tccttccgtc agcaagctgg taccgagagt
    65041 acgggatact gcgaaaatcc tctatggact ttatatggcg atcactgttc tgggcgtgat
    65101 catgctatgc ctgtgcggta tgccgttgtt tgattccctg tgtacgacct tcggcagtgt
    65161 cggcaccggt ggtttcggtg tcaagaacag cagtatcggc ggttactctc cactgataca
    65221 gaatgcagta acgatactta tgatcttaag cggtgtcaat tatacggtat atttctgtct
    65281 gttaagcagg cagtttaagg aagctttttc catcgaagaa gttcgttggt attttttgat
    65341 cattttcgcc agtgctctga cgatcgcctg gaatatccgc ccgctctatg caactttggg
    65401 agagacactg cggcactcct ttttccaggt aggaacgatc atcaccacca ccggatttgc
    65461 caccacggat ttcaatatgt ggccgcaatt gtcgaagacg atccttctgc ttctgatgat
    65521 gatcggagcc tgtgccggca gtaccggtgg cggtataaag gtatcccgtg tgctgatcct
    65581 cttcaaggcg atccgcaagg agctgtccat gatgatccat ccccggatgg tgaaaaagat
    65641 caagatggac ggacacaatc tgtcccacga gacgcttcgt tccaccaatg tctatatgac
    65701 ggcttatttt atcgtactct tcgtttccct gctgatcgtc tgtctggacg aatatgacct
    65761 gagcacaaac tttaccgcag tactggccac cctgaacaat atcggtcccg gcctggagtt
    65821 agtcggtccc acccagaact tcgcattgtt cagtcctctg tccaaatgcg tgctgatgtt
    65881 cgatatgctg gccggacgac tggaactctt ccccatgctc gtgatcatgc tgccgtcctg
    65941 ctggaagaaa tattaattcc ttaatcacaa aaatgagtct gtgatgacat ttttaatcgt
    66001 cacacagact cattttttca tgcatacctc taatcaaatg ttgaccgaaa tgagaacgct
    66061 acatatcatt acaatcatta tatgatgtcg gggtcaaaag cccccgactt tgatgcctac
    66121 ggattgttcc gtgctacgca ctcccacgtc tccgctccgc ttcggtccgt gctagacatg
    66181 tgccactggc acatagcacc ctacccaata ttcgcatatc gtgggattat catcccactt
    66241 ttatgctcat atcggggcgg gtcctaaggt ctttcaccca cctatgagca agctcatgtg
    66301 ggcttagacc ttttgaccct ggcatcatta tatatgaatc ttcagacact tctttccggt
    66361 cagttccttc gtccgttcat ccggctgtcc aaagccaaca taaatatctg ctcccctacc
    66421 ggtatgtaca aatttacctt cttcatctac aacggtaaat gccctcctcg gaatttccag
    66481 tgtcaccatc ttttcttctc ccggtgcaag ttccacgcgc ttaaacacgg caagtttagg
    66541 attgggcact tcgttgatat cctcaacctg tacatagacc tgcaatacat cctctgtaaa
    66601 aacatctccg tgatttgcaa cggttacttc aacacctaca ctctgttctt ccgtctgtgc
    66661 atataccaca tcttccgtca attccgccct cgtaactagt gtatcagcat aattcagccc
    66721 ataaccgaat ggataaagcg gagctttttc cacaaagcga tatgtcctgt tcttcatgga
    66781 atagtcatca aaatccggca tcccctgtaa attgcggtaa atggtaaccg gcaatttacc
    66841 cgaaggtgat atttctccaa agagtatatc tgcaactgcc tttccacctc ttgctcccgg
    66901 ataccatgtc tgaagaactg cattcgccct gttatctgcc aggttcaaat ccatagcact
    66961 tcctgccatc atacatacaa taaacggaac tcccatttct gccacgattt ccaaaagcct
    67021 ctgctgtgaa ggcggaagca tcagcccctg cttgtctccg gatgcatatg cattgcctgt
    67081 atcgccctct tctccttcaa gactttcgtc cagtcccaga cacagaataa caagatccga
    67141 atgttctgca actgtcattg cctcagagat acggtcatcc tctcttgcca gtttttctac
    67201 ctgatctaaa aacaaatggc agccttctga ataaagtacc ctgacatcat cgcctacata
    67261 gtcctgaata ccttccagta cagtaacata ccttgaagcc gtcccatgat aattgccgat
    67321 cagggcttct ctggaattgg cattgggacc aatgactccg attgttttaa gtttcttttt
    67381 atttaagggt aaaatgccat cattttttaa caatacgaca ctcttgcatg ctgcctctat
    67441 ggaaagctca atatgttccc tacattcaac aacttcatac ggaatattat catattctgt
    67501 ctcatcaaac attcccagca agaatctggt cgtaaataaa cgaatggctg atttctgcag
    67561 tagttctttg gaaataagcc cctcttcata ggcattcagg attttctgat aagtacatcc
    67621 gcaattaaca tcgcagccca tctccagagc cagtctgacg ctttcctccg gtctgtctgt
    67681 caccttatgg tgttcatgaa aatcacggat agcccagcag tctgaaacat aatgtccctg
    67741 aaatccccac tgttttctca aaacctccac catcagataa ctatgagcac agcatggttc
    67801 accattggtt ctgttgtatg cgcccataac cgcttcgact tttccttccc tgacacaggc
    67861 ctcaaactgc ggaagatacg tttcccaaag ttctttcatg ctaacctttg catcaaaata
    67921 gtgacgtttt tcctccgggc cggaatgtac tgcaaaatgc ttggcacacg ctgccgtgcg
    67981 cagatattct ccctctccct gcagaccttt gatatatgca acacccagta ttgcaatcag
    68041 tgtcgggtct tctccataag tctcatgtcc acgcccccag cggggatccc ggaagagatt
    68101 gacattgggc gaccaaagtg taattccttt ataaatatca cggtcttcat gtttttcatt
    68161 ttcattatat ttggctctcg cctccgtaga aacggcaact cctatccgct ccagcagttc
    68221 ttcatcaaaa gttgctccaa gcccgattgc ctgtggaaaa acagttgctg tacccgctct
    68281 tgcaacacca tgcaggccct cattccacca gttatactcc ggaatatcca gatgtggaat
    68341 ttcatctgcg tcaaagcgca gctgtgaagc cagttcctct acgctcatct gctccaccaa
    68401 ttcagtagct cgtctgactg cctcttgtct gttcataaat tcctccataa atttttttct
    68461 gattattagc tatataggct taatacggtc tggcgttctc ctgaaaatcc tttcttgcct
    68521 ctgccacaca ggtatcataa tctgccgagt aaggaacttt ctgcttggat ctgaatcatt
    68581 agaaaagctg cgctgtcatt ttctttttgt cttctctgat actctgttgg cagaataccg
    68641 tatttctccc ggaacagtct ggaaaaagcc ggactgttgg ggaaaccgtt ctttaatgca
    68701 atttcgccta tggtcagatc cgtttcctcc agttcccgca atgcatgttc aaaccggatg
    68761 ctccgcagat aatctttata gttgattctg ccatactgac ggaacatttt cgacaagtaa
    68821 gtagggcaat atccaaatgt tgctgccagg gtttccagtg aaatttcttc tgcataatgt
    68881 tcccgaaggt atccagtaat tctttcaaga tgtcctgaat gagtattccc cggataataa
    68941 tccttctttt cctcaggtat ccgataagcg gttaccagaa gatatttcag ctgataaaag
    69001 atactctgca tcaacagttc atagccgtac tcttttctga tctgctgtct gtacattttc
    69061 tcaagtaatg caaaaagttt tccatcctct tccttttttt caccggaaaa ccgcattatc
    69121 tgtttttcca ttgggatctg taaaacaatt gcctcatttc gtcccgatgt atgaatggaa
    69181 tgcacttcat tgctgttcac aattataaac tttcctgcct gaaggacata ttttttatcg
    69241 ccgagataaa aatctacctt tccctcccgc aaggcaaata tttcaacaca gctatgttga
    69301 tgcgcctcac atatatattt tccgtctgca ccttcgaaac ggaatagctt aaagggcagt
    69361 ccccgatccg gcaccactgc ttcctgccgg tatatcttct cttctttcat agtcatccct
    69421 aatattccac accggaagat tttctgttcc atgataaaat ctcaccggca gtttcccgga
    69481 gggggaaaat tctccaaaca gcatttcggc aattacctta ccacctctgg ctcccggata
    69541 ccaacaatcc acgatggctt tcacattcgg gttgtgttcc gcccatggca gatctatggc
    69601 gcttcctgcc attaacagaa ccaccaccgg ttttcccatc gctgcaacag tttccagaag
    69661 ttcttcctga agtcccggca gatttaatcc cagtttgtct ccgctggcat attcattcct
    69721 ggcatcgccc tgttctccct ctatggttgc atccagtcca aggcacatta ctgccacatc
    69781 ggcatgttcc acggacagct ttacttgctc cgtggtacgc actcccactt ctcagctccg
    69841 ctgcggtccg tgccaatcat gtgccactct ctttatttgt aaattctgta atttcccgaa
    69901 agtgccagca tggcaaacag gtacaggaaa ttatcataat atcgtctgtc cccggtgcgg
    69961 agcggagtat tccagaagcg atctacgcat tcctttatat gaggaccgtc tgctgccagt
    70021 gatgcctgtg cattgactgc tacaatggca accggatgca gtgccttctc ttccagtctt
    70081 gttccatcaa tcaggaacac accgtcccag tgttctctct gctcttcaca gtaaaatctc
    70141 tgcagccggt ttgcgatctc cgtctgccac ggatctttgt cataccagag atgatccata
    70201 gcaatgttgg ctatggtgcg gtatgcatca ctgtagtacc agtcatgctt tccgaagatc
    70261 tcctgatgcc cagcatgggg tgtcccgtca tagtctgcgt attctgcgct gagaccggtt
    70321 tgtggatgac atgccttatg cagatattct ctgctggctg ccgcagcctg tctccagaat
    70381 tcccggtctt cttcctccac cttctccgca aacaactcat aaaaatgagg cagatgatat
    70441 gaggggtcag aaaaatccat gtttgtgaca aatttgatca gatgattgga aggctcccac
    70501 ataggttctc ccggatgtcc aggctcccct ttgtggatac attcccgcag gattgctttt
    70561 gcctctttgc tgtaagcaaa aataccttct ccgtcccccc agcgggcaga tgcaaaaaac
    70621 aatgccatgg caaaatactc ttctccgtcc ggtgccggtc cctctgcatt gtgcgttcca
    70681 tccgtgctgc aggaccaggc aaaatagttg cggcagggtc ctgccggaat atacatataa
    70741 gtccgcaccc acttccaaag gcggtcgaat tctgccttct tatccatctg gacacagacc
    70801 atcatgccgt aggacattcc ctctgtgcgt acatcatgat tgccggtatc ctccagatac
    70861 gccatatcac cgcctgcctc atgataaaaa cgttgtgcct ccgttccgta aaatatggtt
    70921 tcaaaggtct gttccagtct ttccccgatt gcggtctcat cataaccgca ctctttcaat
    70981 acatttcgat aaattccagt ctgatatgct cctgccattt gctcgttctc cgatttttat
    71041 tctttgacgc cacccaaagt aagtccggct acaaagtact tctgtaagaa aggataaata
    71101 cagataatgg gcagcatggt aatgatcgtt gccgctgcac gcacggaagt cggtgttacg
    71161 gttcctgcgg cattcttcat tgcctccgct gaagttccct gattggttac ggaggataac
    71221 agcttcatca attcatactg caatgtggtc agattactgc tcatgcgatt gtaaagcatg
    71281 gcatcaaacc aggagttcca ctgtcctacc gccacaaaca gggctactgt ggcataaacc
    71341 ggcttacaca gcggagaata gatcttaaga aaaatagtgc tgtaaccggc accgtccagc
    71401 tgtgcagatt cttccaagct gtcagggatt ccgttcatat aagtacggat taccagcatg
    71461 ttaaatgcgc ttaccattcc agggatcaca tatacccaga agttgttggt aagccccagc
    71521 cccttatata aaaggaatgt cgggatcatt ccaccgttta catacatggt gatgacccag
    71581 aacagggaaa cctctctctg gaacaggaat cttttgcggc tgacaataaa agcaaggatc
    71641 gcattggctg ccagagacaa cagggttccg atcagtgttc tggcaactgt gatataagcg
    71701 cctgtgatca gattatcttt atgtaaaacc gtcgtatagt ttttccaggt gaattttctc
    71761 ggccacaggt agattcctcc ccgcagggca tccgtaccat cattcaaaga gattgccaga
    71821 gtattcagca ccggatataa cgttacgatc acaaacagga ccatgatgat gccgtttatg
    71881 aacggaaaaa tgtcattttt tttggatttg atcttatact ttgtatctat cattgtaatt
    71941 accatctctt tctgttttag aataactgct cttcgccgtt atgttttgca aactgattgg
    72001 ccagaacgat caggatgata cttaccaggc tcttgaaaat acctgcggct gtacctattg
    72061 cataatctcc ctggctgatg ccccatttca atacataaat atcaatcgtc tgggagaact
    72121 tctgtatcaa tccgtttcct aacagatact gaatttcaaa tcctgcattt aatacattac
    72181 ccacattcat cagcaataaa attacgatgg tcggtttgat tcccggaaga gtaacatatt
    72241 taattttatt ccatcttcct gcgccatcaa tggcagcagc ttcatacagg gaagggtcaa
    72301 tggatgtgat ggccgccaga taaataatgg cattccatcc tgtctccttc cagacatttg
    72361 caaatgctac gatcggccag aagtacccag gatgggcaaa gaaattgatc ggctggctga
    72421 gcacatgcag atttaccaat acttcattta caattccggt accagacaac atatcatgca
    72481 ggataccggt tacgatgatc caggaaagaa aatgtggcag ataagagatc gtctgtactg
    72541 tctttttccc accattactc ttgatttcat tcaatagaat tgcaaataca attgcggtaa
    72601 cgaaagttgc cacaaggttg atcacgccca tggccagtgt gttcctgatg acccgcagga
    72661 atgtcacatc cgagaataac atctgaaact tctgaagtcc cacgaattcg gagtgcagga
    72721 ttcccaactg cggcttataa ttctggaatg ccatcgtcca tccggctaag ggaagatagt
    72781 aaaaaatcac tccatagatc atcattactc ctgcccagaa aagcagaact ttttgccttt
    72841 tgatttcttt ccatgtgata ggtgttctgg tttgttttac tttttgtttc gattttgttc
    72901 tcgttaacga catatcctcg ctccttaaaa aacgggcaga tgaaatttta cctgcatctg
    72961 cccattctca aatttcattt atatgttttt atttgaactt tgctgccagt tccattctgc
    73021 tgtccagttc tgcctgtaat tcactgataa aatcttccgg cttgcaggca ttatatgcat
    73081 ccatataatc cgcccatgct ttgtcgaaat cagctgccat aactacctga ggaagatact
    73141 catgctttac ttcaccgatc ttggtccatg ccataccacc gggtgtgctg gtattgaagt
    73201 tattggagaa ggaccacata ggataccatt caccgggtgc ttcgttggtg cccagcattt
    73261 ccacgtaagt ctccgcaccg tatgcctgga atgcttcctt cacatcatcg tttaatccat
    73321 cgaagaattc cctcgcctgg ccatctggct tgtttgcatt gataccatca tcactggttc
    73381 cgttgtactg agggaaataa gaataagagc acatatggga tgccttatac tctgtatcgg
    73441 aagcctgcgt tctctgctcc ggtgttctgt agaattcacc gttctcatct acttcatagt
    73501 caacaccttc cacaccccag aaacgaaggt tatggatatc ctgatccagc agatcattga
    73561 tcagcttcag tgcgccggga acatccttac agcttacagt aatggcaagt ccgcttccgg
    73621 agttgaatgc gccaccggca ttatgccact ggttctttac gctttcatca attgtgatcg
    73681 gcagaggaat gtaatcgcat ccctgtgcat ccagtcctgc ctgcttgatg gcatcgcctg
    73741 ctgtgtaagc gaaatcccac cattgatcga tcatacccag gacacgtcct gtagaaagtt
    73801 tggaaatata ctcatcataa gactgggtaa aggactcggg gtctacaatg cctttcttgt
    73861 attcttcatt taatttactg aaatacttta ctgcggtatc ggaagtgttg taatccagta
    73921 ccgtcagcgt ctcaggatct accatacagc ttccgtcatt aggataacca tccaggaact
    73981 gaggtgcatt ctccagacag aagtatctcc agtcatcaca gagaatggta taaggaatat
    74041 tggctgttcc gtcttccata gtaggatttg ctgcattata cttttcgatc agatcaaaat
    74101 actgatccat ggtccggatc tcgggatagt ctgcccattt cagaactctt gcctgaatcc
    74161 agaatgcttc atcattatgt gtacatacct tctcttcgtt cttgatgcag ctgaactgag
    74221 ggatccagta aatgtggccg tcttcctgac gaagcttctc ccactccaga tcagtaaaca
    74281 gattcttgat attaggataa tcatccagat aatcatccag cgctaccaat gctcctgcct
    74341 catacagttg tttggttcca gtctgtccct caatgaagtc cggatactcg ccaccggcga
    74401 tcatggtacc cacagcttca tctgcggtct gtccggtcag ccaagtctct tttactttgg
    74461 cgcctacctt ctctgcgatc agctgctgca cttcattgtc gtcattgatc tcagatcccg
    74521 ctaccgcaaa aaatgcggtg aattctttga tgcctgcttc ttctgtggta ctgttggttc
    74581 ctgcgctcgt tgtactgtct ccggatgtct ggtttccgtc actgccacat ccggtgaaca
    74641 gtgccgctgt cattgctgac acaagtaaca tgcttacaag tctctttttg ttcatgttgt
    74701 aactacctcc ctttcgataa atctatttta cctttttgag aggaactaac aatcagagga
    74761 actatagcaa aagacagggg aaattatagg tagtcctgta atttcccctg tcttttattc
    74821 tcttactctc cctgttgcat ctttttacgg aatcttgcgg gagtgcagcc cttgatctga
    74881 ataaaacggt tcgtaaaata atccagatca tggtagccca catcttctgc gatctgaacg
    74941 atctttttat cggtacgcaa caaggcatcc gcagcctgct ccatccggta attgttcagg
    75001 tagtctttaa aggattgtcc gtactttttc cggaaaagct gacccagata agcactgttg
    75061 acataatatt tctcactcag gcttttcagt gtaatatttg aagcgtaatt ttcccggatc
    75121 tctttttcca ccatacccag aattcctctg gatacattct tccgcagctg tgacagataa
    75181 tccgcatatt cgcaggcaaa tctggtcaga tgtgccttac ttccgcgctt taccccggtc
    75241 gcaaagctgc tctcactgat gacccttaag atttcctcct ggttcacgtc atcatcctgt
    75301 tcacaggcta agtggatcag ctgaaacaac agataattaa tattcagggt catcgtctcc
    75361 ccacaaatac cgcgtttaca catttcttca taaaagtaag ccaccgcctc ccggatttcc
    75421 agatgattgt tctgttcgat tgccttcagc aggccatcca ggctctgctt acacaggatc
    75481 agtccttccg gagatacctg gacctcctcc tcatagaaat agatatcctt ctttgtccgg
    75541 aacccctgaa aagaacgaag catacaggta ttgccgtaag actttgcgat actgttgata
    75601 tcctgcactt tttttcctgc cagcatcacc acatttgtct gtaaatttga tgcaagatat
    75661 tcccgaaacc gttccagata ccgttttaca tccatcccgt ttttttccgg aacatagtca
    75721 aacaaaatca gtccgatatc gtagattttc tcattgacag agacatcaaa cacacacaga
    75781 tctgcatctt ctttcaggta ttccctgctg tattcataca gccgcctctg taatgtccgc
    75841 atttcccgat ctgtcgttcc ctcttcatca cagacttctt ccatctggat ttccacatat
    75901 ctcacaccga ttccacacct catgtgttca tgtatatatc tggcattcag gtcatcaaac
    75961 ttcccctgga tcaatgcgat catatttctc gccagataag cctgctccag cttccggttc
    76021 ttttcatgat ccagttcctc tctgttgtgc agggcaagaa ccttattcag taccttgcac
    76081 agaatctcgc tgtctaccgg cttcagcagg taatccgtgc agtcattctg tattgcctgt
    76141 tgtgcaaaac taaattccgc atatccgctc agtataacaa aataggcatc tgaaattttc
    76201 tctttacgga tgtgttccaa cagttccagt cctgtcatcc gaggcatttt aatatcggca
    76261 ataaccagat ctaccttttc cttcttcaaa aaagccagtg cttccatccc atttgccgct
    76321 gttcccgcaa cccgaaagcc ttcattctcc caatctacta aaagtttcaa tccctgtaaa
    76381 ataaacggct cgtcatctac taacaatact ttcagcatct gttttaccct cctgcaaaca
    76441 gggaatccgt atcgtaaccg tggttcccac tccttcttct ccttctacac ggaattccac
    76501 tctgttttcc gttaccattt tcaatctgac acaggcgttg ataatcccta ctcttccgcc
    76561 gcccttgagc atctcaatgc tggcattgca catgttgttc tgtagctctg taagtttggc
    76621 ctccgccata ccgcttccag tatcttcaat ttctatctcc agcccttctt cctgacggcc
    76681 tatccttaca aatatccatc ccgggctgga tttgctttca atgccatgca cacaggcatt
    76741 ttctacaaag gttacaatcg taagcttggg tatcttgaag ttttcacatt ctctgtctac
    76801 ttcaatgtca taattcagcc gttctccgaa acgatacttt tgaagcgcca gataagcttt
    76861 cacaaattca atctcctgga agatactcac ggaatcattg ccccattcca catactgtct
    76921 ctgcatcacc gcaagtttgg acaccatgtc ggctgtctcg ttttcttttt tcaaaatgct
    76981 gtgcatccgg atgctttcca gtacattaaa caaaaagtga ggattgatct ggctgtgtaa
    77041 tgccagaagt tccgcatttt ttcttcccac cagtatctcc tgctcacgga tcttattctt
    77101 atatacagtc tcgatcagtc cattggtacg ttccaccatg cgattataat ttcgtatcat
    77161 actgctgatc tcatctttac cgtcttcaca gggcatggga ataagatgat cactgtctac
    77221 cgatttgaat accctgctca gttccgtaat cctttttgta aaggaacggt tgaagatctg
    77281 aacgaaccag aagggaaaaa taacgttcgc cacaaggagc agcaatatga tgggaagttc
    77341 gttccggatg cttgtaagaa aactgttctc taccggcttt acataaatcg taagatccgt
    77401 accatacaga ctgatggtat gttcataggc ttctcttatt gttgcgttat ccagccgctg
    77461 aaactcgctg ccgtagctgc cgtattgtcc attggaaagg aggatccgat ctccttcaca
    77521 gatcagaacc tcattatcat agttcatgtt tttaatgatg cgcaccatac tgccataatc
    77581 aaactcgatt tttaagtact tttctgtctc tgcatcatag aaatccagcc gctgtagata
    77641 aatgatgcgc cgctcccttg tgattctgga actgctgtca tcatagacaa aaaagagccc
    77701 tttgcttttc gcctcttcgt tcagctgcag atagctctct gtattcctga gcttatccag
    77761 cgtattgacc cttcctccgt ttacaatggt atcgttatcc gtatacaggg taaatatcat
    77821 actgttcatt cccagtgcat tttccaataa cgtcccgctc aggaaattct gatatgccgc
    77881 cacatattct gcagaattag tgtatctttt ggataagaac tcttcaaaat cgcgattggt
    77941 ataaatactg ttgcctatct ccccggcatt tccgatcata ctgctcagac tgtattccac
    78001 cgcactggca atattagaca tctcatgctc cctgcgctct ctgtcaaatt ttgcagtcgt
    78061 caggaacaca acactgtctg ttacaatcag gggaatcaac acacagacaa catagaaaat
    78121 ataaaacttt tttttgatgg tgaaatcatc catccaaaga tccagcttat gtaatgcctt
    78181 ccccataaga tcctctcctt atttatgtga aaaagagcag acgtttctgt agcctgctct
    78241 tctttattat aggacatcta cgatctgccc gcaatacatt tttatacggc attctgcaca
    78301 aaccaggcaa aatcagcata gttactgctg tcctgtccat tggcagatgc gtacattcct
    78361 atcgtacatc ccacaaatcc ccctgcttcc tccgtggtat aaggaagcag actgatatcc
    78421 tcagcaatca gcatctccct gccatcccga aaaagccata tccgtgcctt ctgcctgtcg
    78481 caatgcattc gtattttaaa gaattgtcta ccttctccta ccggcttttc ggtaaggatt
    78541 ctctcttccc catggataca ggctgtcaca acaaaataat tctcgtcagc ccttttccgg
    78601 atctccatgc gcagatgatt ttcatgattc tggtacaaca ccattcccgc tgtctcacat
    78661 tcctgcttcg gttcaaattc catcccagtc tctgcataga atgcataatt tttctgacgg
    78721 attccgaaat aggaacaatg attgcagtca gatatttttt ccttttttgt atagagccgc
    78781 agcatccccg gacggtgact cagcgaatag ttttcttcac agatttcttc tacacttacc
    78841 agccgtttgt ccggtttttt ctcccagaaa ctgatatgat cggaagtact gcttacttcc
    78901 tctggaaaac ggtattcctt tgtgggcagt tccaatgtat cttccagatg cccgacgcct
    78961 tctgcaatca ccggccatcc attttcccat gtgactttcg ccagaaaagt ctctcttccc
    79021 atgctgctgt gtccttcaca aggtctggat gccagcatta ccacgtacca gttcccctta
    79081 ccatcctcca ccagatctcc gtgtcctgcg tagatcacag gatagttttt ccccagattc
    79141 ctgtgggtga agatcgggtt tctggggcag ccctcgaacc actggaacag ctttttactt
    79201 cttgcaatgc tgatgctgtg ctccggtccg gttcctcctt ctgcatgcat cagataatag
    79261 taaccgtcga tcttataaag gtgcggtccc tccggccaga taactccttt tacagcccct
    79321 ttccagattg ccatgctttc tcccacaagc ttcatctgcc gaagatccag ttcctgcacc
    79381 cagatttccc agtctccgtt atagcggact ccttccggat tcggtcgtgt accacagtaa
    79441 tagcatttcc cgtcatcatc gaagaaaaga ctgggatcga tccccggagc gtcttcgccg
    79501 agataacaag gatccgacca cggtcccgcc ggatcttttg ccgttacaat gaaattgcct
    79561 ccgtagctta cattggtagt gatcatgtaa aacaatccat catggaagcg gatcgttggt
    79621 gcataaatcc cctgtgatat ttcacttccc ttcaccggaa gttgttccgg tctgtcaaga
    79681 atattcccga tctgctccca gtgcgccaga tctctgctgt ggaaaattgg aactcccggt
    79741 gcatacacaa aactggaatt cacaatgtaa tagtcctctc ctgccctgca tatggatgga
    79801 tccggataga aaccggataa aattgggttc tttgctgtaa ccattttgtt ttcctcactc
    79861 tgttctatat tgtgtatcca aatcctaaga ttgtaaattt tttattctgg tcttcctctg
    79921 tcactgtaat ccgtacttta tgggatctgc tttctgtctc ccaaaacagt aatacagcat
    79981 tacaatgcag ccaattgttc ttatggggat ccgccgtcat acaatataca tcatttacaa
    80041 atacatcggc cttccccaca tccatttctc cggaatcctt aaatataaga aacagtgact
    80101 tgcaggtaat ctccatttca aatcccggca tctgcggtct gcttccatca tacatccagt
    80161 tatacggaaa ttccggtgtc tgtgtaaggc ttccgtccat ttccacactc tgcaattcta
    80221 tatctgtgga agtaaaacta cccgtccgga ttttggctcc tacataacgg ttcttcttat
    80281 caagcaaaaa aattctttca aaatcagcac cgatcactgc cggtccgaaa agcttttccg
    80341 acaggatctg ttcctccgtc attccttcca caaaagtccc aactcttgca ggttctgctg
    80401 catcacaacg ctcaaacaga tattgcaggc agtccgccat aatggtatgt ccggtattat
    80461 tgggatgaaa catatcataa aaaaactgat ttttagataa aattcttccc tcgccgcact
    80521 ttaatgaaaa ctgtggagtg accgcattga ggatgctcac catgggaaga tcataaagat
    80581 cccccaccgg ccttaaccgc tcctgtaaat tccaatcatt tgcaaatacc gaaaacagga
    80641 gaaccactgc cggcttccat ggcagtttca acactttccg caccaggctt tcataacaga
    80701 caccctttgt ctcatctccc tcatcgttta ctgcaaattc aataaccaca atatccggtc
    80761 gttccccttc cctgagcaca tcccggtcaa accggatcat tcccagttct gaaggtgttc
    80821 cgccgactcc ggctttgata aacctgacat tgttctgtgt cgaaaaacga ttctgaaaca
    80881 gttgaaagga cttatatgca tagcattccg tatggattgg tatagctccg gctccctgcg
    80941 tgatagatcc accgatatag gcaagcgtta cctcttttcc tgccttagat ttttcaatag
    81001 ccttacggat acgataagga ttccccagct gcaacagaga gcggctgatc atgccgcagt
    81061 attcttccga atgcatatct ataaacagat cctcttcctc cggaggtgct tcgtatccgt
    81121 catttaggaa gagtcttaca ctgacttttc ccatccgctc cggcgctgca aacagaattt
    81181 taatctgtcc cggcacatga tcatcctctg tccacctgta atccgacaga tagattctat
    81241 gttccatgcc gtcacactta agagaagtcg taagattcgt tccacctccg tacaggtcct
    81301 ttttcccata catctgaaat acaaattctg cggtttcttc cggccggtcc gtctccacgc
    81361 ttacgccgat actatgtacc agtctgccaa atccctctac tgtctccaga agtgtcagtt
    81421 cttccttatc cgtaacattt cccgtgatct gtgcactgtt gatcagccgt ccgtcgcttt
    81481 catagataaa ctggattccg gttccgtcct tttgccgttc tccaaaaata tctttgtctt
    81541 ttaatatata gaaaaaaggt cttgcctttt ccggatcctt cggtgctgcc gatcctgtca
    81601 tttctatccc tcccgaactt ttatcagttc tattatagtc cggctctcct tgcacaaaca
    81661 cccgggaaaa tataggaaat gcaggtaata attttatatc cggcaggctg caaatcgtct
    81721 ccggaaggaa ttccaatctt ctccgtcgct tcgttccatg acaattgctt ttgctttttg
    81781 tccatccgga atatatttct ttataatgtt ttgcatccgt tccggtgtct cttcggcatc
    81841 ggtcatcacc aatatagtct ctacggcttt tcctgctgcc agaaaatact ccataatgtt
    81901 cggtattttc ccctggtgca ggaaacagga cgcagtttct gtggaaagtg cttttaagat
    81961 cattacgctc cgctcttttt ccgctctttc aaaatatgcc ggtgtatata catagatcgt
    82021 acttactttc tcttcctccg caagataatc ataatgtata tgcacctgac cgtgcactgt
    82081 atccgccagt tctgtcagcg ggaactccgg atctggcatt tccagatagt tcacagccct
    82141 gtctcccgag tatcccaccg gagcatccgg gttgattaca tccgtaccat tcgccctgaa
    82201 atacacatta tggtatcccg gcgtgatatt gtggatttcc cctgtccaat atccttgtct
    82261 ttcctgatct ttcctgagtt ttaggaattc ttttccgtcc agggcggctt ctaccgagac
    82321 tgcctccggt gcctcgaagc atacgactgc cgttccctgt tccgtaatgc agattccgtg
    82381 agggatgtcc gggtattttc ctgcaggcct ccctgcttca tcagtctcaa agcggatctg
    82441 gcggtatacc ggatcaaaca tcagtatctg ttcttccatg gtctgtcgct gtagtctctg
    82501 tcttgtgatt ctggctgttc tttcccccgg aatgtcatcc gtttctgccc ccgctttccg
    82561 gaacaataac ggtacaaaat catacaggct tttcctccag acatgccatt cgtgatatcc
    82621 ctcatatact ttggaaatgc aatactttcc tgcattttca aacttttgtt ctatctgctt
    82681 cattcccgtt ctgatctgat cctcatagca tccacaggac ataaatacga catccaatgt
    82741 ttccttgctg tcgcagatcc gttccatctc atgaatagca actccggaaa atacaccggc
    82801 agcagaaaat aacttcatat tttttgccac aatatccgta gtctgcgccg atcccaggga
    82861 aagtcccgcc atcgcccggt aatctcttcc ctttctcacc cggaaattat tttcaatata
    82921 aggaataatg ttgtggatca gttcactctc aaaatttcca gggtaaaata caggtttttc
    82981 tccatctttg aaggcatagc cactgctcat gaccacgatc attttctcgc actttccctc
    83041 tgcgatcaga tagtccatga taaagttcgt cttcccctgc cagatccatc cggtctcgtt
    83101 ctctcctacg ccatgctgca ggtacaatac cggatatctt tcatctcctt cttcatatcc
    83161 ataaggtgta tagacataac attccttcag gtggctgctc accttcgaag cgtatttaca
    83221 gatgctgacc gtaccatgag gaatatcctt tgcaaaatag aaatccacat tcttctcctg
    83281 cacctcaaaa gtattgatcg cagcaaaaca cccataagaa attccggcgt acggattgca
    83341 gatccggaca ccgtccacat accagaaata ataatgcatc ccccagtgaa aatcttgaac
    83401 ttccgcaaaa aatccgccct gcccatcggg catcagattt attttcttat ttgtaaaata
    83461 tcccccaagt ccggcaacct gtactatttt tgctgccggt gcatacagat aaaaaatgac
    83521 tgcgccgtct tctttcactg taacagatgg tcttcctgct ctcttttttt ctattggata
    83581 tctgtccaaa gctccgtttc tcttctcgtt cataaaaaaa tacccccaat ccttttcttt
    83641 agtttaaagg aattgagagg tatttttcta tttgtttttt atactttttt gtaaaaaact
    83701 tatcctttta ctcttttttc cacctcaagc tgcaacatct gataataaat ctcaggatta
    83761 caggaatcat atgctttcat atattgctcc cacgcagtct caaaatcttc tgccatgatc
    83821 acctggggaa gccatttgtg tttcaccgca tccatccgat ccctgacctg tccggcagga
    83881 gaattggcag atagggatgt cgtgtagcta tacatgggat accatgcccc gggagcctca
    83941 ttgttgccga tcatatccac ataatttgtc actccgtaag cagaaaaaca ttcctgaata
    84001 tcctccggtt gatttttata aaattccgtt gtctgatatt ccagtgaaaa cgcattgatt
    84061 ccatcggaaa gccttccttc tactcgtgga aaataagaat aaaaacaaaa gtgttttctc
    84121 ttttcttccg aagtcatcca acgcttctcc tgctcctctg tcagataaaa caatccgttt
    84181 tcatccacgc agtaatcatc tccctctttt ccccagaaac gcagtttaat aatctccggc
    84241 tctaacaggt cactgacaaa ctgcaaagct ccgtcaatgt cattgcagga taccgtaatg
    84301 gataagcctg tagagctgtc catctctgcg gagcgtgtta catgccattg gttggcgatt
    84361 ccttcctctc tggtaatcgg aagtggaaca taatcgcagc cctgttctct aaggccctgt
    84421 tcatcatagg cattctgaat cgaatagtag aactgccacc actgatccgg cataccgcag
    84481 accgcacccg tggataattt tgccagataa tcctcatagg tctctgtaaa tacttctgca
    84541 ccgagaatat ctttgtgata ttcctcattc agctttttaa agtaccattt ggcagtatct
    84601 gtggtgttat aatcgtaaac ctgtaatgta tccggatcca caatacagct tccgtcattt
    84661 ggataaccat ccagaaactg cggaatattt tccagacaaa aataccgcca gtcatcacac
    84721 aaaatcgtaa acggaatgtt cggtgttcca tcctccatta ccgggttcgc tgccacatag
    84781 cgctcgatga gatcaaaata ttcatccaca gtatgaatct ccggataccc cgcccatttc
    84841 agcactctgg tctggatcca gaaggcttca cctgagtggg tcacctccac atcttcgcca
    84901 tgggtcacac caaactgtgg gatccaatag atatgaccgt ccggtctgcg gaatctctcc
    84961 cactgttctt ccgtcagata atttttgata ttgggataat tctcccagta ttcatctaaa
    85021 ggaagtaatg cccctgcctc ataaagactg gtatctccgg aaataaaatc cggatattcc
    85081 ccactcacaa tataactgtt caaggcactt tccttggact gtcccaccag ccatagttct
    85141 tcacaatccg cacctgtcag gcgtgcgatt tcttttttta tatcattatt ctggtcaata
    85201 gattctccct ccacagcaaa aaaagctgtg aaatgcttca cattgttctg cgccgctgtc
    85261 tgtgcatatg caaaatataa tattccgccc aaaagtccaa atactgctac tgctgcaatg
    85321 attctttttt tcaatcgtct gtcctcttat ccatctgttt tgaggctttc agccatgcaa
    85381 tctcctgctt gtaaggtgga atggtcttct tgtcttcgcc ctccgcacac agccatggtg
    85441 tctccaagat tttgggcacg gcggtaaacc gttcgtcatg gacgatccgg tgcagggtct
    85501 catagccgat ggtaccctgt ccgatattag catggcggtc cttgtgggct cctaagggat
    85561 tgaggctgtc gttcacatgg atcactgcga tctggtccaa tcccagaatc ttgtcaaact
    85621 gcgcaagcac accttcgtaa tcattcacca gatcatatcc ggcatcgttc acatggcagg
    85681 tgtcaaagca tacccgcagt ctgtccttcc gctctactct gtcatagatc tttcgcagct
    85741 cttcaaaagt tctgccaacc tcactgccct tgcccgccat ggtctccaga gcaatattga
    85801 ccttgctgtc atctgcgtca agcaccagat ttaacccctt tgcgatctgc tccacggatg
    85861 cttccacacc ggcacttaag gaagaacccg gatgcagtac cagtacctta ctgtgaaagg
    85921 ctgcggcacg ctttacttct tttgccagaa attccacgcc gaaatcaaac atttccggct
    85981 tctccatgtt gcctaaatta ataatatagg gagcatgaat gacgatttcc cggatacctg
    86041 ctttttcggc ataggaaatc ccctcatcaa ttctcagctc tccgatttcc ttgcgtctag
    86101 tgttctgggg tgccccggtg tagaccatca gcacgttggc accgtagctt tccgcctcct
    86161 tcacagagcc taaaaacatt tcttttcccg acattcccac atgggaaccc aataatatct
    86221 cagccatgat ctctccttat ccggaaattc ttttttcaaa atttatcatc tcacagctat
    86281 cagtttggca caatcgggaa ggttttacaa gagtttttat tataccgcct atctcccata
    86341 tttctttcaa taatagaata tcttttaaaa cattttacgg taaaattgtt agcaactctt
    86401 caattttttt aatgtggtaa tctgctttgg cgtacgaata tgcttcacca gttcctaccg
    86461 cctgcatatt tccagccttt gctgcatcaa tacccgcata ggcatcttct acaacaatac
    86521 attcctccgg ttgcaaatca aggtactctg ccgcctttaa aaacacttcg ggatccggct
    86581 ttgattttga gatattggta ccatctgata ttgcatcaaa tgcctcttca agtgccactt
    86641 ttttcaaaat aatcttagct tttctactgg atgagcccag tgctattttg taccctcttt
    86701 catgtaaatc tgccaacgtt tttcgcactg cattgctcac atccgaaggt accatatttt
    86761 caagataaat ccgtaccata cgatattccc agctgctgaa aatactcctt aactgccatt
    86821 atagaccggt tatatgtata gatatcactg tcagaatcca cccgtgcaaa cgtttcacta
    86881 tcataaactg cctgttgcat ttcctgatat aaatgagaat acgatacctc aatattttgc
    86941 agatatgcgg ttacgatatt gctttccgcc cttctgttct tcccccgtac ccatttccgt
    87001 cgattcatct tctcatcctt ctcttttcac tgaccagaaa ggggaacctt tccgcctttc
    87061 ggtttcaaag ttcccctatt caggattatc ttataaaatt cgttcgtata cggctctctt
    87121 cttccggcag tttcaatccc attttcagtc ccaggttctt gcagttaatg aaaaatgcca
    87181 tattgttccc aagggttcgc atggtctgca atccctccag atcctgccgc acgtcttccg
    87241 ggcaggcccc gtgtgccatg ttccaatact gtgaagtgac aatgggcatc tgcatgagac
    87301 cgaaatactt attcatctgg tcccaggttg ctgtggttcc agctcttctg gccgagatca
    87361 cggccgctgc cggtttcagc cggaagtggc tgctgccgcc attcagatcc gcataaaaga
    87421 ctctgtccat aaaagaagtc atggctcctg tggcacccgc ccagtgaacg ggagttccga
    87481 atacgaatcc gtcataatct ccggcaatct ccaggaattc attaaccgaa tcctggaaca
    87541 cacatttccc tgtgccctga cattttttac aggcaataca gccactcaat gctttattac
    87601 caatccagaa aatgtccgta tccactccgt tatcatttaa tgctctggac acctcacaca
    87661 atgcggtgta ggtgcatccc tctctgtggg ggctgccatt gatcaacaat actttcattg
    87721 ttgcgctttc ctcatttcct tattcattca gattcagaga agccacccag tctgccgcat
    87781 ctgcatcgga tgcaccggag ctaaatctgt gaccttcctg ccagtcaccg gtccctgcca
    87841 tctcttccag aagattgccg ctgtcaccca gtccggaaga tgaagaggtg cagaacggaa
    87901 tcacggtctt acccgtaaag tcattgcctt tgacaaaatt gtctaccggc catgctgcga
    87961 ttccccacca gatcggatag cctgcatctg tcaagactgg gacaaaaaaa attttatttt
    88021 tttctgtaca tattctatca aatccttact gatttttaaa gtacagaata gtttattact
    88081 attaaccttt tcgttactac ccatagaaga agccagcagc tttttcacta ctggctttct
    88141 gtattactca ttttgtttac tacactgcat ttcggataaa gttctcaccc ttaaacacaa
    88201 cctctatatc tttgtcgttg taaatgagca ccttatcaat tatgttggaa agtacatctc
    88261 cgtcatactg ttccagcata ctatactggg ttagctgttc tatcttctcc tctgtgtcag
    88321 gctcctgatt tgcagctttc tcctgcaaat ctgatatctg attctccaga tcagctaaca
    88381 gctcttggtt tgtttttctt agctgcacat attcttcttt tgtgtacttt ccctctctgt
    88441 atgactgata acaagaaact gtgctgtttg atattctgtc atactccgct ttaagctcac
    88501 taatcaaata ctcatcgctt gtcaccttcc ttttatcctt tgatgccctt ttaagttctt
    88561 cttcctgtat aagtgttcta cagatttcct ttgtgatttc cagcaccttg tcctgtgctt
    88621 ctgacttcag cataaaaatc tgctgacatt cgccagtcct taccatcctg cgttttggac
    88681 agaggagctt cggtgtacat actgcactgg aattcatcag ctttcttcca caatagggac
    88741 agataaaaag atttttctta cgtttctttg tactcttttc aatccctctg gcttcaaccc
    88801 gtagcatttt attgacttca tcaaacattt catcgcttac tattcttggt attgcacctt
    88861 ctacaacaga ccagtcctct ttatttcgtt tgcgaacctt cttattatct ccaaaaccaa
    88921 cagtttcaca tttattctgg atcattattc ctttgtactg ctcattctgg acaatcgcta
    88981 taacttctga attatcccac atataatccc ctttattatg aaccggaaca taattgattc
    89041 cattcttcca ctggttttga actcttgtcg ggatttttct ctcatttaat atgcgggcaa
    89101 cctcacttgc attatatcct gcaatgatca gtgaaaatat ctccacaaca acctttgcag
    89161 cttcttcatc tattatcagc ttatgcttat cgtccggatc tttcttgtat ccatagcaga
    89221 caaatgatgc cgtgtattcg ccactcagat ttcttgtcct tctggcactc tttacctttc
    89281 ttgataaatc catactgtaa agctggtaaa tcagattctt ataagctaca tccattcctc
    89341 cggtaacacc aaagtttctg tcactgtcat aattatcgtt gacagaaata aacctcattc
    89401 ccattgcagg gaatacaaac tccagatagt tgcctacttc aaggtaatcc cttccaaatc
    89461 gtgaaaagtc ttttactatg atacattcaa atcttccaag ctctgcgtcc tgtatgagcc
    89521 tttggaattc tgctctgtta ttgaacattg ttcccgaata tccgtcatca aagtattcca
    89581 gtacctcaaa acctgcaaat tcctctgttt tcttgatata cgcatacaag agttttctct
    89641 gtcctgtaat actgttactt tcgtcttttg ttcctgcttt tacctcctca tccgcatcag
    89701 agagacggat ataaaaggct aattttcttt tctcagccat tatcctgcct ctctttccat
    89761 attcagttct gcgaattttt tcagttcatc ttcatagttc agaacgatct caagattctt
    89821 gttttcgtat atgcgtacct gtgcaacaaa caaatcgacc atttcctgcg tcaacttgcg
    89881 ttttccttta aaacggagca tttcagcttt taactgggca acggtctttt cagcactttc
    89941 atactgactt gccgcctgcc tgtattcatc agcctgtatc ttcaatttct caatacgctg
    90001 cgaatactcc ctgttcagtt gcaggtattc cttttcatca agcagttctt ctgtgtaatc
    90061 tgcatacaag ccgctcttta attccataag cctgtccatt tccttgacac acttattcac
    90121 ggcttccgta tatacctgtt cctgtctgat taccctgctg tcattgtggt gttcctttaa
    90181 tatcatttcc ctgtcaacaa agacctgtat ctgctctttt ataacggcaa gcaccgctgc
    90241 atatacatcg tctaccttta caggcaggca gttacaacta tcctttccat atctttttct
    90301 atgcgtacac agataaacct ttgcgtgttt ctctgggtga atatggatac tgcttccaca
    90361 acagccgcaa atgattttgc ctttcagcat attcactgtc tgcttttttg ttggctttat
    90421 tacctttctt ttattcttct gtaccgcata gaactgttcc tttgcaataa gtgcaatatg
    90481 atgattttca aaaacatacc actcatcttt attggtagta tgcctctttt cgtgtctgta
    90541 caggcatacc ctgtctttac cagtcacata tcttccaata tagacttcat tctttaaaat
    90601 ctgtgacaat gtatcaacag tccacttaac tccactcagc ttttcaagtt tctctatatt
    90661 atgccttact ctgtactggg caggtgtaag gatatgcttc gcattcagtt ctgccgctat
    90721 cacagaatgc agtgtacctt tttctgcctc attaaaaatc caccttacaa catctgcggc
    90781 ttccttatcc tcaaccatta ccttgacttt attttcatta aagactacct tatagccata
    90841 agccacattt ccagtcggaa tacctttttc catcgcttcc tgcttactgg cagatatttt
    90901 ctttgaaata tccttagcat acagatcatt ggctatattc gtgaccgcaa ccataaggtc
    90961 tgcgtctttc tttgaggaat cataattatc cgtaatcgct acaaagcgga tattcatcat
    91021 cgggaataca cgctctacca gattgcccgt ttcaatgtaa tctctaccca agcgggacaa
    91081 atcctttacg acaatgcagt tgatccttcc tgccctcata tccgacatca tacgctcaaa
    91141 ttccggtctg tcgaatgttg taccggaaat ttcatcgtcc atatagatgt cataaacttc
    91201 catgtccatc tgctgatcca caaagttctt aatgagattt ctctgattga tcagggtttt
    91261 tctgtctctc gtttcatctt tttccgcaga taacctcaca tatatggcag tcttatacac
    91321 tttggataaa gccttcagcg tatccgtgga ctgtttcggc tgtgttcttg acttccttgc
    91381 catttaaacc accagccttt ccgcatcatg ctgtctttcc agctccccgg ctcttgcaac
    91441 gatttttctg tattcatcgt cacagtccag tacgatttct atatcaccgc cctcatatac
    91501 ctttacagac tgtataaggc tcactaccac attacgggta agagaaggca ttgttttcca
    91561 tttcctgtgt tcttccagcc actcggtatc tctttcaatc ccggcagcaa tgttttccgc
    91621 ttccttctgc aacctgtcga ttgccttttc cgcctctgct ctttttacct caaaactctc
    91681 cttgataatc gcaaaatctg atttatcaac aattccatct cggtaatcct cataaagcga
    91741 aacaagcatc tggttgtatt tggttatctc tttctctttt gcactgatcc tctcttttgt
    91801 ctttttctgg tctattctga aattggcact attgccagct tcctttatta cctcatctgc
    91861 atccagaagt attgctgtca tatccctcaa ggtatcaaat acacggcttt ccaagtctgc
    91921 ctcttttatc ctatgggagc tgcacttctt attcttcttg ttattggaac acatatagta
    91981 aacatacttt tttccattta ccgtagacac ctttcgtgtc ataagtgccc cacagtcggc
    92041 acatactgct attccggata aaagatagat agaatctttt cccggtgcca ttctcatatc
    92101 cactgacaac agacgctgta caatctggaa agtccttaaa gataccagtg cctcgtgatt
    92161 gttgtggcat actgcccatt tctcctgtgg ctttaatatc tttgttttaa ccttatggtt
    92221 tggtgttgta aattttccct gtacaagtgt tcctgtataa ataatattcg ttgcgatacg
    92281 ccttaccgca acaggagtcc acagcagctc gtctttcttc tgaaatgctg tcttataatt
    92341 ttcgccattg ctgagtttgt atgccatagg tgacaggata ccaagaacat tcagcttatc
    92401 gcttatggca tcaaggctca ttccgttctt tatccagcgg aaaatgtcct gtaccacatg
    92461 acctgcatat tcgtccggga caatcacatt atggtcaatc tcagatttct tgtagccata
    92521 gacacagtaa tttcctacaa attctccgtt ctgtctcttt gtttcaaggt ggcttcggat
    92581 ttttatggaa atatccctgc aataagcgtc attgataaga ttcttaaacg gaatgattat
    92641 ctcgtttccc tgtgcctgtg tatctgcact gtcataggca tcattgatcg ctataaatcg
    92701 tatccccatt gcaggaaaca tccgctcaat gtatcgccct gcgtcgatat actctcttgc
    92761 aaacctcgaa agatctttta caacgataca gtctataata ccgtctttca catcctgcaa
    92821 cataagttta aatgccggac ggtcaaaagt tgcccctgaa tagccgtcat cctcacggat
    92881 tgatactatt tcaatatctt ctttgtcttt taaataatta agtatcaggg atttctgatt
    92941 tgagatactg ttactttcag cttttctcac atcatcaaga tcgccatctt ccttagataa
    93001 tctaacatag atggctgctc tgtaaattct tttagaattg ttcatgtcca ccactccttc
    93061 gattttctaa gttagaagtc ccaaatcaag aggaatggaa acataaatat gaaattgata
    93121 ccctttcggg ttagatgtcc ttatttgtct gtaatgatat cacaaatcca tctgaatttc
    93181 cacccctaat tttggctgac tgctctatac attggcaagc atagcaatat aattgtcttt
    93241 caggctttcc ccattatcgg aaaatgaaat cttaatcttg ctctttcctg acctgcggaa
    93301 atacggatta cctgtctttg caatcattcc cgcaaccttc ttttccacag gcattccgtc
    93361 agcgtacttt atgctgttta cttccaccaa tgtatcaata tccacatctt ctaatctctg
    93421 tgccgatatt ttgtccaact gttcaactgt catcctatcc ctccaatcaa attaagccgc
    93481 cagatatgct gacggctatg taacaccgga gctttcgccc cggctggaat ttatgcaatt
    93541 ggattattat gttttgtact catacccaac tggatctgca taccttttgt tatctgtctt
    93601 aactggtttt tgcttacttt cccgattttt tctatcaggt ccgtatcttt cacatcacat
    93661 aactgctcac actgagcgat actgttcgtt cttaatccaa cagccttaaa cgaactgata
    93721 aaaatatgtg ttggcagata tgtcttttta tgaatcttac ttgtaagcgg tactgcatgg
    93781 aataccgtgc tgtgtctgtt tgcactgtca ttgctcacaa tcactactgg tctgataccg
    93841 tgcagcttgt ggtcattctc atcttccggt actccaaaat caaccatcca gatatcgccc
    93901 cgtcttattt cactcataat attcacttcc ttgtctgtca actggtattt cctgcccacg
    93961 gatatattcg acagcttcct gttccgtagg acaggcaaca actctgtctc ctttcccatc
    94021 aaacaccacg gtttctgatg aaaatgtcac tatgcttaat tcctttttat ccggcattgt
    94081 cctcctcaaa tgagatgaga gcaggttcat agcccctgct ctcgtcgtta atgctgatcc
    94141 cgtgctctgc tgcaagttcc atgatacctt tcaccacatc tttatccgat gagacatcat
    94201 agatactgct taccagaata ctgataatgg aatctctttc gatataaaat ttaagtcttt
    94261 ccacagactt cgtatcttta aagattgttt ctacaatctc aaaatccgct ttcttcgcat
    94321 atgcttctat cctgtctgcg gctctgtcgg catttctgcc tacgcagata acacaacact
    94381 tactcataga gcctcctatc tgtcggctct gagacgcttg aaaaatactg ccataaaatc
    94441 agcaagtgaa acaataccgt tatcttcctt tttccctgcc tgctccatct tctgataata
    94501 tccagccata tcatcgtttg ctgtctcaac cgtaatgccg tgctctccga gcataatgat
    94561 gttatccata attgtttctt cacaatcgcc caccatatca ctcataagag taacaaccac
    94621 atcaacatct tctacttctg ccataccaac aaaggcaaac ttcataggaa atgtcattcc
    94681 ctccggtctt gcgttctccg aaatgactgc aaccacctca tatccattca cagcgcaata
    94741 ctcctcaaga acctgcatta ccatctgatc cgctgcctca tttcctgttg ctccaaaata
    94801 tataactgct ttattcattt taaaatctcc attcctgttt ttattttgat ttgatatcct
    94861 gtgcacactt tatctcctca cataaactga cctgccaatg ctctctgtac ttataagccc
    94921 cgttgttgtc tgctggttac tgcaaatcag tttgtgtggg aagataaaat actatcttcc
    94981 cggtttctta accatccccg tatcgggtaa catatccttt gccagccaca atcacttcgt
    95041 agtttcctgc ccatctggac ttacgaaagt agccactctc tgaaaatgcg ttttgctgaa
    95101 attcaggtgc acttattatc tcattacctc cggctgctaa cccggtaggc acaaccctct
    95161 gctcttatcc ttaaaggctc ctgctgttac gtttctcata atcggctaca gctctgagga
    95221 tagtcatcgc acgaaatggg attctgccac cctgtcatag acaaggcgat ataagctcgt
    95281 gggatatgcg tgtcctattc tgttttcaat attcaacggc tcttttctca gaacctgaac
    95341 aaagtataac tgacaccacg catcttctac agtaaccgac actcttttga actaagagta
    95401 acactcccac ctgttccaaa tccttataaa aaaagccgga ctgcattatc aaaaatgcaa
    95461 tccgactttt ttattctgtc agaagtttca tattgtcaac ggcatttaat accatattcc
    95521 ggataaactg tacttccttc tccttcagac caaccagcag atcatcgact tctgcctttc
    95581 tttcacaccc acacacaaga taatccaacg ttatatgaaa caattcagca atactaatca
    95641 gcgtatctat ttttgctcca tttacaccag cttcaatttt ccgcagagca tccactgaca
    95701 atccaatctg ctcggcaagc tgctgtcttg tcattcttct tgccgcacgc aactctttta
    95761 ttctttctcc actattaaca atatcgtagt acaccttcca ctcttttccg gcaccttata
    95821 tatttgctta agctgtattc attgtaccgt aagcgaatct tttgtaagag tgactaaacc
    95881 agctatttca ggtggaatat tgtacacctt aataccaaga atataaatat ttgcggatta
    95941 aaaaagagat atttccaaac tctcattaca ttttggaaat atctctttac ttaatatcct
    96001 catccggcac aaactccatg atgtcctcta tcttacaatc aagtatggaa cataatctgt
    96061 taaggattac cgtttttacg accatattct ttcgcaatcg ctgtaactgt gctttatcta
    96121 ttccataatc tttaattagt gcatactggc taatattttt cttcttgagt gtgatccata
    96181 acctgtcata tactatcatt acaaagacct cctccttgta ttctacgcaa gagtgtccta
    96241 taatgatatg gtgcgtgtat gcaccatact gtatgtatat gatagaatag aaaatagcca
    96301 taatctgcat agaaaacagc tctcaaggtt attatgtaat atagaacgga ttatctgttt
    96361 tacttttatg gtattcaacc gggacaattc aagcacctga caatttgacc gcaaataccc
    96421 ttctcagaaa ggattcacaa caacactatg aatgataaaa acacatactc tgttgataaa
    96481 tatttggaaa tcattgccga aaaagaagga atcacaaaag aagaagtcca acaggaaatt
    96541 gggcgggcag tttccattgc tttaaaaagc cctgatccaa aaatgcaacg cttctggaca
    96601 gactttccat gtgaaaatga tactcccacc attgaagaaa tcatatacca tctcgctgaa
    96661 aaatttgcaa aagagtcata gacaaaggac aggataacta cccgtctatt cactcagata
    96721 atcttcctgt cctcttaata tttgttcaat catatgtaat gcaaattcta tttctctttc
    96781 gtcacatcga ttcatcataa atgcaaaacg ctcaatgtgt ttttctgggt gctgctggct
    96841 catcaaaacc ataggcgatg actccaatgc atgaacaata ttcaaatatg tatcaagact
    96901 cattgccctt ctgccatttt caacactttt gatagtatcc aatgatacat ctgcccgttc
    96961 tgcaagctct gcctgtgtaa ttccaagcct tctgcgttct tttctgatat tctctgcaat
    97021 aatttcgtat atgtgctctc ttttccccat agccccatca tcctttctga cttatcgcat
    97081 tatatcagaa attattatgg gttttaggaa actgtaatcc tttgacattt tcgtgtaata
    97141 ctttgcatat tccttctaaa caccaatcta cttttacact gaaatttaaa atttcagcaa
    97201 aatgtatata acaacatgag aaaagcccgt ggcagatcca cgagctttat ctccagtttg
    97261 aagcatggat atagatagtt ccctgctttt atttagtgta acatacacct ttacttagct
    97321 acatgtgtaa aatcccagtg cgaaaatacg tctacatagc aggtttttgt ttcaaccaca
    97381 tatctctcca tgccgcctaa tgcttcatta tatctttctt tatattttgt ctcaacgacc
    97441 ttatatgtat aatacatagg acgatatttg attaaataat gagatccttt tggaacagtt
    97501 acggagtatc cgctacctaa agaatatgat tttgatgcac ctagtgtaac acccaactca
    97561 cttcctatag ttccaccatt tgtatatcca ccggaaactg aagctttaaa ggaaatatta
    97621 taagaagtat cttgagtgta attcaatgat aattttgcgc tagatgtaga actaccacca
    97681 ctcgtgccat ttctccatgc accacttttt aaactacctt tggtatattg ataaccatat
    97741 acggtaacaa aatcagaaat gcgttcttca ttttcctcat cagctttaat ttccgtttca
    97801 aaataatcag ctccatcatg aggcaaattt tcagtgtcat agttatgagt ctccgcagca
    97861 aacgctgttg tcgagcaccc caaaatcaat ataaaactta aaaccatact aattattttt
    97921 ttcattagaa gtcacccccg ctttgcttta aaaaaactat aaaccagata aataattgcc
    97981 aaaatgatac taaaaacaaa aaagccacca atataaataa gaggactttt aaaaagataa
    98041 tccatgttat tggtaagata tccatttcct gaactaaatc cagttattga gtcaatatat
    98101 tggacaataa tggaaagaaa cggaagcgta cttaaaccaa atatgccaaa aattatcatt
    98161 attatagcta aaatcttcaa atgaaaatct ttcatcaaaa tcacccccta ttttaatatc
    98221 attttgtcgt tatatgcaga tcattctata agcatattca taattatatt tacttctcct
    98281 ttcctcagta ttcaatgtgc catatagagc aatctgttgt atattctaag aatagcagga
    98341 atcaaagcaa aagaaaatat cctatgcaga aagtgacctg tttttgcaga aaatgaccaa
    98401 cagaacaaaa attactcaga aatcaagtaa aaaacacagc attgatacta tttcaaagct
    98461 acagttctgc tctaaagaat atattgagag tacgctttca acaatatttc ctagtctaaa
    98521 aacggtatta gaagaatttt ggtaaaaatt tagcaggcaa taatttgaat attattgcct
    98581 gccaaattaa tgatgaaatc atatgattct tctgtcaatc agaaccaatc tgctttctcc
    98641 tatcggagtc caagcataca cactcttgca atataacgta tcatcagctc cgagcctgtg
    98701 ctttctgtat attgtctgac caatacttat tgctgaaaaa attcccattt ctaggcaaat
    98761 ttctgaacat atccataaca atatcatatg ataagcctga tgtgtccaca aacatctctt
    98821 tcccatcttt agatttaact gcaattccgt tatcacccac atacgctact gtattgtcat
    98881 ctaatttctg tattaatccc gtcatttccg caaatttctt tagcgcaaac tccccggatt
    98941 cttcacaaag ttttcggaac ttttctgcat cctcacctac catatacgga tattttccat
    99001 ctctaatata ccatgaaatt cccgtttctg aatccgtgta tttaggatca gttaatgccc
    99061 actcttccaa aggaacaccc tgataccata ctgtcatttt ttcactggaa tcattgctta
    99121 tttctggttc atcattttga atttctttct cagcaatttc atcctgcccc tgttcatatc
    99181 ctctacatga cacgttacac tttagtgcct cataaatttc ttgaacattt gcatatttgt
    99241 taactgctgc aacatccggt gtctctaaca cttccttttg ttgaaaagcc tttgattggt
    99301 tatattttga acctgatttt cttcctgcgt aaatattgtt atttacagcg gtatttacca
    99361 tatttaccat atttataatc cctccagcag caatataatc taattttctt attatagtat
    99421 gttgcacttt atgccagatt gctataaaat gcaacataca atgaaaatat tctgttgcgc
    99481 ttgcttaata tcccatattc aagcagctat tattgtgcat aagcagcaga agcaatatag
    99541 cctacctgcg aagacccaac agaatacaac tcataatgga aatggtatct aacgcctacc
    99601 ttaacacctg taaatgtcag tgtccatgaa tctcctgaat agttagcaat ttgtaccggt
    99661 gcacttgttc cgtcatttcc tacaagagta attgttccct gaagataatc tgttccactt
    99721 tcattaggca tataaacatt taagcacaac tttgtaacgc ttgagttatt aatggtgtaa
    99781 taataatttc cgttgaatac accgcttctg gaattgcttc ttgtaatggt tctatttgca
    99841 tcatactgaa tcgtatctgc aagagcaacg gcgtcatcat aagcaggtac tacgtccaaa
    99901 tcgccacaat cgcttacttc tactgcctta tttgcattag gggcagcctg tccctgagtg
    99961 gatacttcac ttgcaaacgc agttgttccc attgccaaac ataacatcaa actcaacacc
   100021 aacgccttta aactttttac tttcttcatt ttaaaatctc ctttcaaatt aacatcgtat
   100081 aaattttcat tccaacagga atctataaac aactttatgc ttttttctgt ctttccagat
   100141 ttagataagt attcatattt cgcatataaa acatctgcaa atcatacttt cctaatttgt
   100201 tcatatcctg aataaccttt tcaaagctgg atataaggtc acatctttca tttaatccca
   100261 tgtgaccagt ttcttgtgga aaagagctga gactattacc cctatcaaca tcataatatg
   100321 cctccaaagc actcatttcg acataagaag catttctcaa atcaatatcg ttaatattaa
   100381 tcttttcttc aaattctttt ccattttcat caatcccttt ggctatcact acaggattat
   100441 catctgttga ctcttctgca tacttaatat aaaacgaaag accggttcca tttccgcctg
   100501 aaaacaacat atcatccgtc tttaaataca ctttcattga tgtctgagga aatgcctcat
   100561 ttccgttaac agatgttttg ctattgtttt gtcgctttcc aaataattca gatacactct
   100621 gcactcccga accaatacgc atttaaccac ctccattcgc attataatca gtatagacat
   100681 tgtcaaatac gttggcaatg gtgtcggcaa ggtactgatt gatatcccct tccttgttgc
   100741 cgtgcatgtt catattgact accatgacac caccgtcctt cagatgatct ttgaccatgg
   100801 taaagaattc cacggaagac atctggaaag gaatggtgat gtcctgatag gcatccacca
   100861 tgatgacat
//
LOCUS       DAWEJW010000002        80203 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_148_length_80203_cov_61.854806, whole genome shotgun sequence.
ACCESSION   DAWEJW010000002 DAWEJW010000000
VERSION     DAWEJW010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 80203)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 80203)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..80203
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148_length_80203_cov_61.854806"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    48896..48995
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    54558..54657
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        18916 a        22703 c        16271 g        22113 t
ORIGIN      
        1 tggcactcat tttttgcaaa ccggttattt gttacttaac aggaatgctc cttctgtaaa
       61 aatatatatt ttgcagggtt tttcttttgt actctttcct ttttgctctt ttcctcttgc
      121 tcttgtacgc atatcctatc tactccttaa tattcggaac agctccatat cgtcctttta
      181 agtaacctag ttcaatatta tctttttctc taattaaata atcgtccaga agcttaacat
      241 atgttgctcc atcttcatcc ttatccacta aatagatttg ttcagggttt aacaaattga
      301 gaattttggt atcgtgtgta ctgaaaaaca gctgtgcctt tccacaattt actttattat
      361 taaacaattc tatgaaatac tctgttaact ttgtatgata atttctgcct aactcatctt
      421 ctataaataa tcccccttct ttatttataa aaagcatttc agcaatatta tctataatgt
      481 cacttgttcc agcggaatca tcattaattt caatatcaaa tgtttctccg ctcttgctag
      541 catgagtaat ccaaatcact ggtctatctt caggtattaa atcttctgaa acctcacctt
      601 ctcctaacat attggttagg aattgaagca ttgcatctct atgttgcttt tctatcttct
      661 tattctttac atatatatct tttatattaa aatctgcctt ggaaagtgcc tctaatagca
      721 tatctttttg agaactatct ttcactacat ctgctattac ttggctaata ttcatatcac
      781 catattgcct aaacattttt tcaaaccatt tgtatggagt ttttatcttt tcatatcccc
      841 actctgctgc aactggcaaa tatagtctat ttgaatttgt tttttgagct atctcatttt
      901 gaaccttaac atcattcccc ctaaaattat attcaggtgt gttgtttaca tctctctcaa
      961 aaagtgtcgt tgccttcttt gaataatacg ctaaaagata ctcctctaaa acacgctctg
     1021 tatctattgt aaagccataa acatacttta tattattctc gcaaaaaata aactcgaatg
     1081 acgatccatc ctctctacta tctttatcaa gcataaacat ttcaatatta attttagatt
     1141 tttcttttcg ttctattgta gaattaataa atttcaaagc aaaaaacaac gaattaataa
     1201 tgttactttt tccacttgca tttgggccta ctaaaccaac acatctataa acagagctcc
     1261 ccaactcatc actaagaagt ttatctgctt ctacattttt ttgcatagca gtaaaattaa
     1321 taattatttc atcttttata gatttataat tctttacaga atactgtatt aacatatatc
     1381 tacctcccaa attattaaca ttgttttatg tacttttttc tccgaatatg ctttgataga
     1441 gttattttaa actttatcac ttatccccgc atttgtcaaa ggatattatt attaacaata
     1501 ctattatagg taattttatc aaatttattc ttatagaagc ttattttcgg caccgtttta
     1561 gcaccgctga ctacactttc actgctattc agacgtaaaa aggctcccag aggaaaacct
     1621 ctggaagcct taattttact gcatttatct ataatcagat attgattact cgataatagt
     1681 agcaactctt cctgaaccta cggttctacc accctcacgg atagcgaagg taagaccctg
     1741 ctccatagcg ataggatgga tcagttcgat ggtcatctct acgttatcac caggcatgca
     1801 catctcaacg ccagcaggca gttcgcaaac gccggtaacg tcggtggttc tgaagtagaa
     1861 ctgaggacga tagttgttga agaaaggagt atgacgtcca ccttcatcct tggtcagaac
     1921 gtaaacctga gcggtaaact ttctgtggca ggtaacagta ccgggtttag ccagaacctg
     1981 tcctctttcg atttcagttc tctggatacc acgaagcaga gcaccgatgt tatcaccagc
     2041 ctgtgcctca tccagcatct tacggaacat ttcgataccg gttacaacag tcttcttggt
     2101 ctcttccttg ataccaacga tttcaacttc atcattcaga tgcagaacac cacgctctac
     2161 acgaccggta gcaacggtac cacgaccggt aatggtgaat acgtcctcga cgggcatcag
     2221 gaaaggctta tcagtatcac gctgaggatc agggatataa gaatctacag tatccatcag
     2281 ttccatgatc ttgtcgcccc actcgctgct gggatcttcc agagctttca gagcggaacc
     2341 cttgatgata gggcagtcgt tgaagtcgta ctcttccagc tgctcggtta cttccatctc
     2401 aaccagctcg atcagctcgg gatcgtcaac catatcacac ttgttcagga atacgatgat
     2461 gtaaggaacg cctacctgac gggacagaag gatgtgctcc ttggtctgag ccataacacc
     2521 atcagtagca gctacaacga ggatagcacc atccatctgt gcagcaccgg tgatcatgtt
     2581 cttaacgtag tcagcatggc ccgggcagtc aacgtgtgca tagtgtctct tctcggtctg
     2641 gtactcgatg tgagcggtag aaatggtgat accacgctct ctctcttcgg gagccttatc
     2701 gatgttatcg aagtcaacct tctcgttacc aggtactctc tcagcaagaa ccttggagat
     2761 tgcagcggtc agagtagttt taccatggtc aacgtgaccg atggtaccaa tgttacaatg
     2821 gggctttgtt ctgtcaaatt tagctttagc cattatttaa gtcctcctta aaataattaa
     2881 acagtttcgt agttttattt ttttaactcg ctacctttga ttatattaaa taatcagagg
     2941 tttttcaagt cattttattg atttaatgct tattttgctt ttgcagcaag aaccttttcc
     3001 tgaacgttct taggaacggg ctcatacttt tcgaagaaca tggagtagtt accacgcccc
     3061 tgggtcttgg aacgcaggtc tgtggagtaa ccgaacatct cagcaagggg aacgaaagcc
     3121 ttaaccatct taccaccgcc gatgtcttcc ataccttcta cacgaccacg acgggagttc
     3181 aggtcaccga ttacatcgcc catgtattcc tcgggcatgg taacctcaac cttcatgatc
     3241 ggctcaagca gtacaggagc tgccttctgc attgcttcct tgaatgccat ggaacctgca
     3301 atgtggaatg ccatctcgga ggaatcgacc tcatgatagg atccatcata tacattcgca
     3361 tgaacaccca gtacagggaa tccacccaag ataccagcct gagctgcttc ctgaatacct
     3421 tcacctactg cggggatgta ttccttagga atggaaccac caactacagt agattcgaat
     3481 aacagagtag gaggatcgtt gatcagaatg tttgccttag gatcaaattc aaacttctca
     3541 cagttagcct ccaggggctc aaacttaact ttacaatgtc catactgtcc acgaccaccg
     3601 gactgcttag catacttgga atccacttcg acagccttgg taaacgcttc cttataagca
     3661 acctgaggag cacctacgtt agcttctaca tggaactcac gaagcagacg gtcaacgatg
     3721 atctccagat gcaactcacc cataccggcg atgatggtct gaccggtttc ctgattggta
     3781 tgagcacgga aagtaggatc ttcttctgcc agttttgcaa gagcttcacc catcttgccc
     3841 tgaccggctt tggtcttagg ctcgatagcc agctcgataa cgggctcagg gaactccatg
     3901 gactccagga ttacaggatg ctgctcatca cagatggtat caccggtagt ggtgaacttg
     3961 aaaccaactg ctgctgcgat gtcaccggag taaacctttt ccagctctgc acgcttgttc
     4021 gcatgcatct gcaggatacg tcctacacgt tccttcttgt ccttagtagc attgagtaca
     4081 taagaaccgg aattcagagt accggaatat acacggaagt aagcaagctt acctacgaac
     4141 gggtcagtca tgatcttgaa tgcaagtgca gcgaaaggct cttcatcgga agactttctc
     4201 tctacttcat taccgtcaag gtcaacaccc ttaatagccg gtacatccag aggagaaggc
     4261 atatactcga taatagcatc cagaagcttc tgaacaccct tatttctgta agcagtaccg
     4321 cagcatacgg gaacggctct gcactcacag gtagcttttc tcaaagcagc cttcatctct
     4381 tcaacggaag gaatttcacc ttccagatac atcattgtca gatcatcatc cagctcacaa
     4441 accttttcaa tgagctcgtc atgatataaa gcagcctcat cagccatatc tccaagatca
     4501 tcggtaacag tgatgtcatc acccttatca tcgttgtaga tgtaagcctt catctcgaac
     4561 agatcgatga ttcccttgaa ttcgtcttcc ttaccgatag gcaactgaag gcagatagca
     4621 ttcttaccaa gtctggtacg gatctgatct actgctgcgt agaagttagc acccaggata
     4681 tccatcttgt tgatgaatgc catacgaggt acattgtagg tgtcagcctg acgccatacg
     4741 ttctcagact gaggctctac accacccttt gcacagaaga cgccgacagc gccatccagt
     4801 acacgaaggg aacgctcaac ctcaacggtg aagtctacgt gacccggggt atcaatgatg
     4861 ttgatacgat gctccaaagc accgggcatg ggtttggtct ctttttccag agtccagtga
     4921 caggtagtag ctgcagatgt aatggtgata cctctctcct gctcctgagc catccagtcc
     4981 atggtagcag taccttcgtg agtatcaccg atcttgtagt taacacctgt gtaatacaga
     5041 atacgctcgg tagtagtggt cttaccggca tcgatatgag ccataattcc gatatttctg
     5101 gttctctcta acggatattc tcttccagcc aagttttttc cctcctacta gaatctgtaa
     5161 tgagcaaaag ccttgtttgc ctctgccatc ttgtgcatgt cttcttttct cttaactgca
     5221 gatccggtat tgttggctgc gtcaagaatt tcgttagcaa gtctgtcttc catggtcttc
     5281 tcacttctct tacgagagaa catggtcaac caacgaaggc ccagagcctg acgtctctca
     5341 ggtctgacct cgataggtac ctgataggta gcaccaccga tacgtcttgc ttttacttcc
     5401 agaacaggca tgatgttgtt catagcctct tcgaatactt ccagagcagg cttgccgctc
     5461 ttctcttcaa ccttagcaaa tgctccgtat acgatcttct gggctacacc cttcttgcca
     5521 tccagcatga tgttgttgac aagcttggta accactttgt tgttgtataa aggatctgct
     5581 aatacatctc ttttctgaat atgtccttta cgtggcacgg ttcttccctc cttaacaaat
     5641 tgttctcgtg agatccgtta tctcggcatc aaagatgcct cgatgtcggc tgttcgccga
     5701 ccaatccctc cataaaaagt tcgctcgtaa gcaagcttac gctctctttt catgtcggtc
     5761 ttgtcttctc actagttacg ttaaatcatc ggtactcaca tgtgtttccc caagtgttcg
     5821 cgctgttata tctgtatcac agaattccaa cacttttctt agtttcgttt ttgtggtaca
     5881 aagatgtctc gctgacatct ccaaaaatcc tcgcggattt tcatcgaact ctcggtccga
     5941 tgttcaaaac gccccggata ctacttattt cttagcatcc ttaggtctct tagcgccgta
     6001 cttggaacga gcctgttttc tgttagcgac accggcggta tcaagggtac cacggatgat
     6061 gtggtatctg gtaccgggca ggtccttaac acgaccacca cggatcaaaa caacgctgtg
     6121 ctcctgcaga ttgtgacctt caccgggaat gtagctggta acttcgattc cgttggaaag
     6181 acgtactctg gcgatctttc taagagcaga gttaggcttt ttaggagttg ctgtcttaac
     6241 agcggtacat acaccacgct tctggggagc agaaacgtct gttgctttct tgtgaagaga
     6301 gttgtaaccc ttctggagag caggtgcagt agacttctta acagaagtct gacgtccttt
     6361 tcttactaac tggttaaatg ttggcattct gtttcacctc tttcttaata gtataataga
     6421 cgcacctttt ctggcacgct ttaacagtat acttgttccg acttccgttg tcaacaggtt
     6481 tttcgtgaaa tttacagggt ttttcatgac tttttcatat attataagtc gagtaggctg
     6541 gctcctactc gatttgtata ctttgtacac catctcgctt aaaagctcga tgatgtacat
     6601 tcgccaaaat gggagccgta ccgtttccga taccgctccc tgaagtattt cccgttattt
     6661 tcctgcttac acaaagtctg tttacaggat ttctttcaga tatctctcta acgcatcctg
     6721 ccaggaagga agccttacga atccgttctc tgtcagcttg gctttgctca tgcggctgtt
     6781 ctccggtcgc ttggctttcg tcggatactc ggaagccggt acaggagcca catccacttt
     6841 catgccagcc tggcggaata tctcgcaggc aaattcatac cagttacaga taccttcgtt
     6901 ggtagcatga tagataccgt atttatccgt caggatcata tccaccagaa gccttgccag
     6961 atcataagta taggtaggag atccgaactg gtcattcacc actgtgagat gatcatgggt
     7021 cttgcccaga ttcagcatgg tcttgatgaa gttcttgccg ttgactccga atacccagga
     7081 gattcttacg ataaaatatt tctccagtgt attctgtact gccagttctc cttcgtattt
     7141 ggtctgtccg tatacattca gcggatgacg ctcgtcctct ggctcccagg gtctggtgcc
     7201 ctcgccgtca aacacataat cggtactgat atagaccatc ttgcagtcca gcttcttgca
     7261 gacgttcgca atgttctgtg tgccgtccgc attgacctta cggcagattt ccacgttatc
     7321 ctccgctgca tccactgcgg tataggctgc acagtgtacc acagcgtccg gtgccgcctc
     7381 tccgatcacc ttttccacgc tggccgcatc tgtgatatcc atctcctgaa tatccacgcc
     7441 gacagcctca attccgcgct ttgtcaactc atttaccaca tcatatccca actggccttt
     7501 tacgcctgtt accaagactt tcatagattg ccttcctttt ctttcctatg cttgtggcgc
     7561 aggtctctga accggccaca tacatgatta taacattccc ggcagacatc agcaacgaaa
     7621 tacttcactt ttataaatct ggtgtaaaaa atccggggat gatccccgga tctttctgat
     7681 taaatttaaa cagtcatata gctttgcgct gcccgccgga tatcctcgac aattccttct
     7741 gcctttttat cctccagttc ggttacctgc tttctaagtt tccaccagtt ccagccggga
     7801 tgtgcatttt cctgcaattc tatgattctt ttctggtatt ttctgcataa tataaaataa
     7861 atcaccaaga gtaataaaac accaatgccc agcattccgg ctactgtgtc accgtaatat
     7921 ccactctttg ctgcctgtgc aagcggtgtc gcttcatctt caatttgtac gattcgataa
     7981 cctgtcaaaa tcacaggtat ggaatataac atgccgctca tctcgaccgc ctccgttatt
     8041 tcttagtctc tatctataaa gactacttat ccatgaaaaa cggctcacat ttttcgatat
     8101 ttttttcagg aattcctacc ggtctgcttc cggaaatttt tatcgcataa atttactttt
     8161 tatgggaaat ccgatataat ggtgatataa attttcacac ggctatttgt gctgcaggcg
     8221 tcacaaagca gcccttatcg cagaatcaaa tttgaaattt gattctcgat ttctccgacg
     8281 gaaggaatcg tctgcagaat ggagattatt atgaaaaaaa gtttcatacg acatattttt
     8341 gctgtaagta tattgtcctg ttgtctgtta ttcggaggat gcagtcagaa ttctgacgga
     8401 gaggatacat cctccgccac gactgcttcc acctccccgt ctgagactgt tgccgcccag
     8461 cctgccgatg tttccccgga ttccgattcg tctgccatag aatccacttc ggaggatacc
     8521 agggcactca ctctgtcagt agtcaatatg tgcggcatcg aaatcggtat ggtagcggtc
     8581 attgatcccg tgaccgggga acaggtaaat ctggattccc tgtcggatca ggaatcgatc
     8641 tctatggaag cagactggcc ctcctctgtc aatgagttcc aatgggctct ctacaatact
     8701 gacggtgaat tatgcgttga ggggacaacc gatatcagca gtgcccaggc cacagtgact
     8761 cttcttctga ccggagacgg tgatctggaa aaagtcgaag agctcttcga gtaattttct
     8821 ttccaaggtc taaaaaacga catccaccgg ggaaatccca atggatgtcg tttttgttat
     8881 cttttattct tcgatatctc ctgcattttc tgcctgagcc ttcagcttct tctcattgtg
     8941 cttcttatac atttcatatc cggttgcacc gaggatcaga atgatcagcc accaccacca
     9001 ggacatcttt gcatgttcgc tttctacggc aacatctgcc aggggaacag cctcttcttc
     9061 gatatttacg gcttccttgg tctcttcctt atcggaacca gcctgaacgg tttcctgtgt
     9121 atttgcagga gctgcttctg caagaggtgc tgcttcgtca gcgatctgta caacgggtgc
     9181 tgctgcctgg ttctgtgcca ctacggttgc ctgtgctgcg ggtgctgctg ccaacgcaac
     9241 aggtgtaaca actgttgctg cctctccggc gccgccttcc tctgtatcac tggcaccccc
     9301 ggtctcacct tctccacctg caggtgttcc gggtgtaggt gcaggagtca gacgatcgat
     9361 caccttatcc agctctccgc ctgcctcatc caagctgccc agaatctcct tcagagtatc
     9421 ttctgcatcc tttttgttct gttcagccac ggccagcttt ccttccagtt ccttcagagc
     9481 tccaaggtta ctggttctgt tactcttcag agcttcgatc tctgccttca gatcattgac
     9541 cttagcctgc gctgtttcca cgtctttctg tgcgtcctgt gcatcctgta acagcttctg
     9601 atacttttgg gtcagagcct tggcatcatc tacgaactta cgatattcag tatccttctg
     9661 ggtcagtttg atgttgccct gaatgtaatt caggttctgg atgatctgac cggtcagcac
     9721 ctctgcattt gcataagtct cagttctgac agcagtattg gttgtggtct ctccggtttt
     9781 ctccagataa tcaatctcat aggaatagct ggtatgagca atggtatcgg tatctgtagt
     9841 cttcacattg tacacaccgg ttgcgccgct tgcttttgcc tgtgccagtg ctgcatcctg
     9901 taccgcattc tgtgcctccg ctgcggtggt cttctcatcg gtcttgacac tgacacctgc
     9961 tacatagcgg atggttgcct tgccaagctt ccagtcatcc cagttagcgc tgacgaaaat
    10021 accgccatct gcctcgactg cctttctggc tgcatcttcc agtttcttgt tcgtagtttc
    10081 tccattacca aacagagcct tgatgtcatt ccagagggag ccgaaggtac tctggtctac
    10141 tgtcttctta gtgacctttg cataagttgc ggatacagtg ccggatacga ggaagtcgga
    10201 atcatcatac cagtgatctt ctgtatagcc ggttacctta agatcactgc ttacaccgat
    10261 caggtataag tcgtcatcat cgttaatctt attgcttaac gccttctcct gctctttcgt
    10321 aacctttgct actgcttctt cctctgttct tacaccataa tccgtctttt tatctgtatg
    10381 cttccattca acctcttcat ccttcacatt cacagtcttc gtaaaggtag gaatgtaggt
    10441 tccgttaccg gtataagtat aatccgtttt ttctgttcct gtaacggtta catccttgcc
    10501 ggcagcgtcc ttcaaatcct tttcctttgc agcagctgca gcatctctct ctgcctctgt
    10561 ctgatactgt tcggtagaag tgaattttgc atcggtatag gtgatggtgg tcacatcagc
    10621 ggtcacggtc ttgatcaggt tgccgttctc atccagtttc caggattcct tctcggtagc
    10681 ctcatttaca tcagtatcaa cctcgccgtt ttcggtcttc tggttattgt cagaaataat
    10741 gatactctcg gttccgtcgt tcttgatcac tttctttcca tctactgcaa taatggttcc
    10801 gtctttcaga cctttttcca cttcggaaac agtgatagta tccccatttt ccttcacata
    10861 ctgatccgga ttggtctcct gcgccgtctt ccagtttact tcctcaattc gcttctcaaa
    10921 gatcacgata ttgtccttgg aagtctgttt gtcatccatc acatagttgt aaaactttgt
    10981 ctgcttatta ccattctcat cggtataggt tacttcaaaa taattcttgg tattattgtc
    11041 ttcaccctgt ctgcggacaa ccttaatatc atcggcagtg atcttctgta cctcgggcag
    11101 atagtagttc tgcataatgg aaatgaaaag cttatcctca tttttccagt taagtccctg
    11161 atctccctgt gtcagagctt ccttatctgc aatatccatg gcacctgctg cggaggtatc
    11221 cactgcgctc ttggcagctt ccagagcctt tgccagtgct tctgcatttt cctgcgctgc
    11281 tttcaactca gtagctgcgg catctgcatt ggcatctgca ctgtttaccg catcctcata
    11341 tgccttttca taatccgcca gtttctttgc ggcttcttca taagctgcct tggcggtatt
    11401 gtactccccg agctttgcgt caaaatctgc ctgtgcctga tccacggtag tctttacttc
    11461 ttcataggca gcattggcat cggtgataga agcagcatcc ttgatattct cgatctgtcc
    11521 atttacctta tcctgggaag cctgcatggc atcctttgct tctgccgcag tctgtcctgc
    11581 atttgccgca gcatcagctg ccttattcag ttctgcgtcg gacagcttgt cattggcttc
    11641 cgcaaccccc agcgcagtat ctgcattctc tacggcagac tctgcgtcct tcagggatga
    11701 accgtcttta acagtcttat cctctacttt ggcattggca tccagaatgt cctgagacag
    11761 atcctttccc tcggcatcct tcacatctcc ggctgccacc ttgtcaacca cttccgcctt
    11821 cacatcagcg gcactctttt cggcgccctc tacagccttg tcagcatctt ttgcgactgc
    11881 ttccgcctga tcgcatacac cggcttccgg tgtcactgta atgttcttat cctcgtttcc
    11941 ttcggaagaa ttcccctctc cttctgctgc cagtgcagtc aacggtgaag atgccatcag
    12001 cattgcggaa atgccgatcg ccatagcacg aatcactttt tcgttcttac tgttttttct
    12061 catatgattt ttcctctcca ttctattttt caacaatatg ttcaaataaa tactgcagca
    12121 cctccggcgt gaacttcttt gccgaaatag ctactttaaa atttccgtct tcttgctcct
    12181 cctgtctggt gacatctgca tacatgatca tcgtatccgc tacgattatg atgtctttcc
    12241 ccttaaggtc aggagatcct gccggcatcg taaaggctat gcccagcgga ctgacattcc
    12301 gggtctccac aaaaatgctt tccccggtat cacataccac gataacactc tttgcctggt
    12361 agcccacacg gtcgatccgt cttcgttctt ccatgtcccc ttttgctcct ttcttccaga
    12421 gatgcctgtt aaaacgcccc ttttattctg tcttatctat taagacttcc cgtctcagaa
    12481 aaaaggttca gaaaaacaga aatatttttc tattttttta catttttttc aaaacattgt
    12541 attcttagta tttttcccat tccttttcct tacactcacc tccatggcca gcagatccgc
    12601 aagcaggatc atccctccca gcaccgccgc caacggttct ctccaccatc catatccaaa
    12661 caataccacg gcaccggtca ttcctaccca gttcacccgg gttccatgca tcttcattcc
    12721 acctcttctg cctctctttc taatacgcat ccgcatgctt taacaacact gtcaccgtct
    12781 tgcacaaaat ccggatatcc tccatcagtg accagttatc aatatattcc acatccagct
    12841 ccacaatctt ttcgaaatcc gtcaccagac tcctgccgtt cacctgccac atgcccgtca
    12901 agcccggctt gatactgatc cgccgccact gccttgtgcc gtatttctcc acctcgtccg
    12961 gggtcggcgg tctcgttccc accagactca tggaacctcc caacacatta aaaaactgtg
    13021 gcagctcatc aatgcttaaa ttccgcagga tcctgcccac ccgggtgatc cggggatcct
    13081 cccggatctt gaacattacg ccccccgcga tctcattttg cgacagcagc tctgactttc
    13141 tctcttccgc atccacatac atgctacgga atttatagat ctgaaatgtc ctgccgtttt
    13201 tcccgacccg ggtctgccgg aagatcacag gaccggggga atccagctta atggccagcg
    13261 ccgccaataa catcaccgga gaaaacaaaa cgatccctgc caggcctcct gccacatcca
    13321 tgatccgttt tatcacctgt tctccggtat tcaacgtcac cgtatggtag gtgatgaccg
    13381 gataggttcc cacacagctt acataactgt cggctctgcg cctcttatag aaatccacca
    13441 ccatccgtac cgtcaccccc atatcaatgc acagatccac atatttctgc atggaaaaaa
    13501 gctgttcctc gcgcttttgc agaatataga tctgatccag atggtaccgg cggatcagca
    13561 tttccagatc ttccagactt cccagatatt ctcctgcatc tccttctgaa atctgtgcca
    13621 gttcctgtgc cgatgccgca atatacccca caagctccat ggggatgctg gtcttcttca
    13681 aaaaataccg aaacttgttg tactgcgccc ttcttcccac aaatgcggta cgcggcactt
    13741 ccccgcgatt tctttccatg tctctgcagc atctgccgtg tcgcagcaac ggtacgcttt
    13801 ccagactgta cgccaggatc agaaacagaa aatatcccct ctgatcctct gtaatgtatg
    13861 gcagcaggac cacggttgcc gctgttgcca ccagaaacga tcctgttctg cgccgctgta
    13921 tcctgtccgg ataaccgaac agggtcacat tatacagata ttgctcttcc tttcccaata
    13981 cataaagcag cacgaatatc gctgcaagtc ctgtcatacc cccaagtgtc cgaaggctcc
    14041 atgctcttcc cataaataac acagccactc cataagccac cagaagccgc agcatatcct
    14101 ttactatgtg tccgatatcc ttatttgcac tcgtatttga ccgattcatt tttctccccc
    14161 ttctcgtgtc actttttcat ccggtctgtc gttttccgct gtcctttgtc tctctctgtc
    14221 gaccttcctt tggtgacaat cgtttttccc ccatgctaaa atagcaccag caaaggaggt
    14281 aagataatac tatgttcaag cttgtactga tcgatgatga tgccgaagtg ctggcgatca
    14341 atcagaaata tctcaccggt gcgggattca ccgtgtatac tgccatctcc ccccgggaag
    14401 gcgtcttatc cattcgaaag aatcttccgg attgtatctt actggatgtt atgctgcccg
    14461 aagccgacgg ttatcgggtg tgccgttaca tccgctcttt ttcctctgtg ccgatcctgt
    14521 ttctcactgc cagaagttcc gaggaagatg tgatccgggg acttacctgc ggcggggacg
    14581 attatctggt caaaccctac agtctccggg agctgaaagc ccgggtggac ctcctgatcc
    14641 gcaggcgcag acaactggcg caggcttctt ccgatctggt actgacgatc ggaaatcttt
    14701 ccatcgacag ggtagcccac aaggccttct accgggaaca ggatctgtgt ctcgccaacc
    14761 gggaatatga agtcctgctg tatctggcag aacacccgga ccgggacatc accttcgagg
    14821 aactgggaac ggctctcttc ggtgtctacc gggaaagtga cagacgcagc gttatggtca
    14881 atgtcagccg tctccgcaaa cgtttcgaag gcaattacga attggagaac cgcatcgaga
    14941 ccgtctggtc caaagggtat cgttttctga aaggaggtat ctgaccatgg cacaaaattc
    15001 ccgccccgtt ttccgttctc cctctctcga acaggagacc gtggaagaac tgtcccgcag
    15061 gctgctggaa atcaccgcgc agttaaatgc cagcaatcgc tccctgcagc acctgcaaca
    15121 ggaacgtacg gaaatgctgg ccaatctctc ccatgatctg cgggctcccc tcactgccat
    15181 ccgcagtgcc gtggattatc ttacttccgg acagtctctg tctgcacagg atatcgaagg
    15241 tgccctcacc ctgatcgatc accggactgg gacactggaa catctgatcc gggatatgta
    15301 cgagttgttc actctggagg atccttccca cgccttttcc tttcaggaac tggatgcccc
    15361 ggcctttctg gaagaatatt tctataccgc acttccggac agccactatg ccggtcatct
    15421 tctctgttta tctgtatcgc aggatctcca tgccaccctt tttgcagatc ccggaaaact
    15481 gatacggatt ctggacaatc tgctcggcaa tgccgcaaaa tatgctcccg ccggaacgaa
    15541 aatcactctg tctgccgcgc cttccgctga ccttacttct ctccggatct ccgtgaccga
    15601 tgaggggccc ggcattccgc cggaggacct cgagcggatc tttaaccgca cctacaccgt
    15661 ctcctccgcc agaactcccg gttctgccac cggcagtggt ctgggactgg ccattgtaaa
    15721 gacgatcaca gaacgtcacg gcggaaccgt gtcctgcgaa aatgcctccg gtgcaggcag
    15781 tatcttttct gtgacacttc ctgcaaaatt cttacaatca tgatacattt ctttacttcc
    15841 gcccctgtgt tccctatgct gtagtcacac agggactttc cccgtgtaca tctctgcata
    15901 aggcagacgg aaaggagaat tcttatgaag cattggacac aacctgaact gatggaactg
    15961 aaggtatccg agaccgcctg tggatggttt ccctggtacc ccggatatcc cggcggagga
    16021 ggacatcatc ccggacacca tcccggtggc ggcgactgtc agcccccgca gccttctgag
    16081 gaaccggaag agttcttaag ctgacacgac gtttcgcagt gcgtgtactt ccgctgccac
    16141 ctcggcagtg gaatcacctg ctgccggtct ccttatccaa aagaccggta tctgctccgc
    16201 tattttcagg cagttctgcc agatttccat tcccggatcc tgttttttcc acagatgtct
    16261 taagaacaga ttgtctttat acacgatcat gctgtcgggg cctgtgattt tttctgcttt
    16321 tatcttcccg gacttatctt ccgcctcttt tttccatcgg attcccagaa aaacaaatcg
    16381 ttccagtctg gccttttccc tttgaaatac cccggtacag ggcaccagaa atttgtcctt
    16441 tttttcatca atatacaaaa gttcttccgg gggatatccc tgcctcagca ccacatccct
    16501 gcacagcttc atataaggaa aagccggata cacccacacc ccttcttttc cggaaatgtc
    16561 caccactgcc atatcgtctg ccagaaaacg atacccccgt ttcagcagtt ctgccgtcag
    16621 tgtggacttg cccgcgccac tctctccagc gatcaggatc gcaccacctt ccggagttgc
    16681 cagcgcactg caatggatgg gaagttttcc ctgctgaatg ctgagcattg ctattccaaa
    16741 gcccagcaga taggttcgta ccgctccggg atctcctccc ttccggatgt gataccggat
    16801 cacctgtccc cgctccacac tcatggccag cgtacgatta cacaaccagc ctttttctgt
    16861 acctaaaaaa cttccgcaga cgcagtctcc gtgttctttc cgaaactgtg cttcctcctc
    16921 ttcgtccatc gcccggatcc ggaccttttc ccgtccgaag tccctgtcat cctccggtaa
    16981 acgcaccgga tccagctgta caaagtccag atccgctgtt accctcatgc cgtacagata
    17041 gtagtggtac tgcattcttc cgtcacactc ccttcccgga tgcaaagttc tctgtcacaa
    17101 tattttaaaa cacccttcct gtgggtgata agcaggcagg tgggtctcgg ttctaatgcc
    17161 cggagcccct gcaatacagc gacctccgta gcttcatcca gcgccgaggt ggcctcatcc
    17221 aggatcagaa acggtgctct ccgcaaaaag gctctggcaa tggcgatccg ctgcgcctgt
    17281 ccttccgaca ggccatgccc tctttccccg atgacggtat ccagcccctc cggcagtgac
    17341 cggacaaaat tttctccgga agccagtttc agggccgccc acaattcttc ctccgaagca
    17401 tcttctctgc ccatctgcag attttctctc acggttccgc tgaacagagt attcccctgg
    17461 gggacatagg caataaactt ccggacgaaa gctccggtct cctgcctctg tccgctgcca
    17521 ttgtagaaat ccaccgttcc cgctcccgtt tccagaaagg ccatcagaat gcggatcagt
    17581 gttgtcttgc cgataccgga ctctcctaca atggcgacaa attctcccgg acagatcttc
    17641 agattcaccc gtttcagcac cggttccctg gtatatccga agcatacatt tcgaagctcc
    17701 actccgatct cccatccacg gatctctccg gctgcccttt gctccccggg aattctctgt
    17761 aactccatga tcctcccggc ggaagtcaac agggcaatga tccggggaat ctgctgggcc
    17821 agtcccagga tcggcgcctg tacccggttc accagtgtca gaaataccga catagtaccg
    17881 taggtgatct gttgtctcga cagctgccat gccccgtagg aaaatgctat cagatatcct
    17941 ccctgaaaag ccagtgacat caccgtggaa ctggcggttc ccagtccggt cttgcggtat
    18001 acccagtaga accgttcctc ccgcagcttt gtcaggcgct ccaccgcccg gtcctctccg
    18061 gtaaaagcct ttacgatcag aaggttggcc agactttcct gcaaaaagga acggtattcc
    18121 gactcactct cctgcacttt cccctgtaac cgtttcaggc gttttcccag ccaccaggag
    18181 cacagggctg ccaccggtgc caccagcagg gcaaacaccg caaggagcgg ggaataataa
    18241 aacaacatac aaaacaccag taccaactcg atccccagct gtaagatatt ggggatcgtc
    18301 cccacaatac cgtccgccac atttcccgca tcgctggtca gtcttgtcat gagatcaccg
    18361 gtatgatagg ccgtcacttc ccgccagcca gaatgcagga tcttctcata gatctgccgt
    18421 cggatcccga aggaaaaccg ctccgttacc agtgcagtaa gcagtgtcga caacaccgag
    18481 attccctgca ttcccaataa cagcagcaca taacccgtaa gcagccgtcc gaaaccgctt
    18541 cccactgtgg catgatcaat gatctccttg gtgatctgca ccatggccag cgaggcaaaa
    18601 gtctccaaca gcccaaacaa taacagcaga ccaatcctgc ccgcataggg agcggtatat
    18661 tgccacaacc aggaaatata atctcttcct cttatttttc ccatatgtgt ctacacctct
    18721 tccaggatcc ccgcctgttc cagtttgtcc agaaatgcca gcacctcttt ttcacacacc
    18781 tcttccatca cttcatattc ttccatcagt ttatgtaaga tagcctccac cgttatgccg
    18841 tcttcgcctt cctgcccgtc cagcatatcc cagatgtcgt ttcccacacc tttgatgcag
    18901 aaatatttcc cctgttcgaa atccaccatg accttttctc ccgccaggtc cgtcacattc
    18961 cttttcgctt tcattcggat ttttgccgtt ttgtccattt tctgttctcc cttcttttac
    19021 acgctctgtc tccgtctgtt aatcgagcag gcggactcat catatacacc aaaaaagccc
    19081 atgtaaccat gagcttccct gatccattct ggcattttcc tctcatacag tccaaaaact
    19141 actgtacgtc tacggtaggt tctccggaat tgccggaaga aggaccgtaa gtggggatag
    19201 tatacttacc gtcatcggac tggtaaacgc cgtcctcgat cagacctccc ttagggctgt
    19261 aagccgttgc tcttacgttc agttcctcca tttcgggctg actccatgct ttcattttcc
    19321 attcctcctt attacatttt tatgatttct tacatttctg attataataa tttccctgta
    19381 aaaataccct ctttctaaca aaactgtaag attttcgtta cgacctcgtg gagtgtatcc
    19441 ttgtccgact gcctgatcac ttcatagatt ccggcctgcc ctgcgatccg gaccaactgt
    19501 tccatcaaag gatacggaat cccggtactg taccggtagg tatccgccag atacaactgg
    19561 ctgctcaaag tctccagtgc tttctgcccg gtgagctttt ggatactcac tgcctcttcc
    19621 ccggtattct gcaccgcatc ctgccacccg gccacagcat cctttcgcag caaaaaaatc
    19681 ttcccgagga gcgccgcctc tttccgatag caatcctgtc tgcgccaggc atatttgtcc
    19741 ctctcttcgt caatatagat cagttcttcc agcggcttcc cgcatttcag cgccatatca
    19801 cgacaaagct tctgctgcgg gtaggaaggc tccgccagca tccccatagt tcccggatgc
    19861 accctcactg tgtcatccgc cagaaatcca agctttcttt gcaacaattc catggccacc
    19921 gtagacttcc cggccccgct ctccccacag agtaaaatgg cctgttcccc ggtatacaat
    19981 gcacttccgt gaaaaaaagg ttcccccctc tgcagacaga ccagtgccat cccggcactg
    20041 agcaacaggg aagtgatcac aagcagttgc ggttcctccg aaagctcaac ggtgatccta
    20101 ctgccccctt ccacatagac ctgtgttccg tcccggatgc ggaaccaggc cgatacaaag
    20161 ccgtttgtcc aggtcccgta gcccttctgt cccgcttcca gcaggaaatc cggcatcctt
    20221 ccgaatacaa tttttacctc tgcctctccc gccgtctccg gtactgcctc cgaaaagggg
    20281 atctgcgact gcacccgcag tccgtagatt ctgtaaaaat attccatcat acctcaaacc
    20341 ttctccgata ttcctgtgcc aggcccacat aaaagccacg caccatgtct gcttttgagt
    20401 gcccgttttc cccggacatc gtctcacccg gaatccccga cagttcctca cagatccgtt
    20461 cccactgcaa ggcgagcagt tccccgcttc ccacgccctg tcttcccttg tggaattcat
    20521 ccgccaggat ctcttccggg atcttcccct gtaaatactg ccgaaccagg cgtctctcct
    20581 gcccggcatg ggtgaactgt tccgccggga gacttagcac cagcacgatc agccgcttgt
    20641 ctctggtggg atctctgggc accactcctg tggccagaga cagctttact tcatattctc
    20701 cgatctgccg caaggcactt ttgtcataca tataccggga caccgcttca tgatccccac
    20761 cggcttccgc cgtgagaaga tcccgccaga tccacttgcg gctcctgcgg tattttctgc
    20821 tgtagacatt gatctcccgc accagttctt tgattcttcc ctgatgtagc agtgtcagga
    20881 acaggcttcg aaaatcccca taggatatgg taatatttcc gtactgtccc gacagcagga
    20941 tcccacagtc tttctgagct gccagacgat aactttcata catccagggc agattcagta
    21001 ccgctttaca gggaacttcc agaacttccc gcagcctccg gttctccgcc agcagatctc
    21061 cccggttggt caccagatac tccggttcca gattcccgtg aaatctccgc accagttcca
    21121 cactgcttct ctcatcctcc acagcgtact gtcccgggat ccgtgctctt tccttctcct
    21181 ccggcaccgc agtaaaggaa tacagtttct ttcctctggc ggcaaggtaa ggtgctgcat
    21241 aagccgccac cgcattggag tccagcccgc cgcttaacag aattcctgtt tcttttccac
    21301 cccggccttc ccggatcaca tcctccacgc agtgccggaa caccgtggtc accagctccc
    21361 ggtaccctgc ttccgtcttc ccccgcagga tcctccttcc cgcaaaggga tcccagtaat
    21421 cccttctgtg caccccttct tcagtaaata ccacaatttc tcccggagcc agtcgcagga
    21481 tccccgcata gggtgtctct ctgggttccg acaccgcccg cagatcccgg atggtataga
    21541 accggtcaaa ccagcccgta ttttccttcc agttctcccg ttcgcaggtg atccccgcaa
    21601 gcaacgtgga aaaatagacc cttttttcct cctgaaaata atagaccgaa cggtttccca
    21661 ccgcatcatt gaacaaagtc agggtccgct tccccggctc cagatacgca caggcatagg
    21721 ctcctctggc cacggcaagc gtttcctccg gatggctgca aaagctttca tacagaattt
    21781 ccccgtccgg atggctgcac agatcccgga tgcctccaaa ccgctgcacc agttctccgc
    21841 ggttatccag gatcacatcc gccacgaaca ggcttctgcg ctctcccttt ctttcataag
    21901 ggaatctttc ttccacagct tcccttgtga cattctgtac cccgcaggcc agataatagg
    21961 ttgcctccct tttttcctgt atccggtcca ggcagctcgt cctataggcc tgccgcatcc
    22021 gcagagcctc ttcccacagg ttccctgcac gattttttct ttgtgcctct gactgcgcca
    22081 cactcagatc cacaattccc cagattgccg acatacttat cccctctttc ctgaagcctc
    22141 cttacagaac cgggccacgg tagtatatcc gccgccgtcc cctccggtca catacatttc
    22201 cccaaaacgc agccaggcat gggccaccat tttaccttct tccatgccac accccagata
    22261 cagggtggaa gaaagtcccc gccggcacaa cagtctctgg gccgccagcg cccgcaccag
    22321 acatttactc tcccagggag tcttatcaca gacctttccc acgatctggg cgatacgcgc
    22381 cgcctgtcgg tatccttccc gggatgccgt tcctgcggat tcttcgtttt ctctgcccca
    22441 gcccttccgc agtttccccg gcggcaggag cagcagctgc atgcggaaga tcgccgaata
    22501 ataataggta agtaaggttt cttttttcca tgggttcttc caaagaaacc ctgacggact
    22561 tttcatggct ttcttacact ccttataaat atttcaatgg aacagactaa tgaaaacgta
    22621 actgcagtct gcggtagata tcctgtctta caccgatctg tgatgcatcg atctgccgca
    22681 gcatttcccg cttatatttt ccccggccgc agaccacacc ccggacggcc cggattcccc
    22741 aggctgccgg aagcagaaat tttcccgcaa cgggatttct cgaaagccac gggtaataca
    22801 acaccatata ttcataagga gggaaccagt accagcgttt cagggcatct cggccctttt
    22861 ctcccttctc cggctgcgca tcgcagaacc ggttccagat accgttttca tccttgccat
    22921 agattccaca cccctgcata tagtcaaaca gttgctgata aaacgcttca ccgggctctc
    22981 cctgcagcca gatccgggcc agcttctcca tatgctccgt aaatgccgtc agtcccaatc
    23041 ccgcaaactg cttttgcaga tagtctgcgt tcatttcccc accgaatttt tccagaaaca
    23101 cataaatgtc caccagattc cggataccac agcctctctt gtaaaaatgc tttgccatat
    23161 gagccatcat gtaaatatag aaatcttccg cctcaaaatc atacacatac agaaaaccct
    23221 ttctgcgcac cgcccgggac agatccgaga aataggcata ctgcctctcg tccactgtcc
    23281 ggtcatacat ggctctgtgc gcctccacca cgattcccgg tgctttccga tagatatcat
    23341 gatgcttgat cttctgttgc aggacatacc ccatggcttc cagttccact gccgcctgct
    23401 ccatgaaatc tccgtggatc agaatgtcaa tatcactcat ttcccgcaga aacggagacg
    23461 gataatagaa tttcatccgg gctcctttca tgggttgaca gggaatcttt gcctgttcca
    23521 tccgttcctc cagcttcttc aattcctgca gctgcatacc ggtaaaaagc atgcttccca
    23581 gtactttttc ccgcaaaagc tctttttctt cctccgaaag cccatctgtt tttaacaagg
    23641 ctcccaaaag cagataatcc atatggttct cctctgaaat ctttacaatc tcccttacag
    23701 agactccctc tggcagagac gccgcctttc tgtcctccaa tccggccttt accagttccg
    23761 ccagatattc tgccattgtt tccatacgtg gtcccctttc cctttattct tcaggtgcgc
    23821 tttccgtgac cgtttcctgt actccgtcag gagcctccgt ctgatccaca agcgttataa
    23881 gctcttccgt atatcgcaga atatattctc cccgtgaggc aaaatattcc tgaatcgtat
    23941 ccccgtcagc cagatatcct tcctctgtct gtcgcaggcc gtagcgctcc gccgtggctg
    24001 tcactgccac ccggtaagtt tccagaagcg gtgtcagata ctcttctgcc ttttccttcg
    24061 tcagttcttc cacgtattcc cgcattctct gccggaacgc tgtctgaaag tccttatttt
    24121 tcagcaatcc ctgtaaaaac agatcttctg ccacccccgg acacagatag gtattcacac
    24181 tgtaattact gaccttattc cgtcccagag tatccgtggc atccgagaat tcccagtgcc
    24241 attttcccgt ctctccttct cctgcccgtc gccacaaagt ggtggaaaaa gagtcaaact
    24301 gcgaattacc gaaatagaga ttggcacagt aataatccag atagctttcc agatccatct
    24361 gctccaggat cttctgatac gttccgtgtc cggaagtatt cccctcggtc accagccggt
    24421 acagttcccc gtattccgga gcgatctggg agggatagcg gttctctatg acctgaattt
    24481 cttccgggga accgccggct cttcgggcaa aggtttccgc cgtatttacc ctgctcacca
    24541 gatacaatcc ccagaactcc ccgtccacaa acaccagaca gcccttccgc tcttccagcg
    24601 taaaatccgt gatttctttt aacagcagtt cctctaaaac ctgtctgttc ttggccacat
    24661 gatccgtgcc gcccccgtac agaagaaacg tgccgtcccg gaaaagctca tacagctcac
    24721 aactgctctc cggcaccgga tccgctcccg tcataagaaa acttttctgc ccgtaatcca
    24781 gatttccgtc cttccgcaaa cttaaaatta actccccctc ataagtcaga tatctgtccg
    24841 cttcgaagta ttccacatgg gcattcatct ctccaccccg gtaataattg gcagaatcaa
    24901 accgcaggtc atccgccgcc agcgcattct cataatccac acctgtgaca taattgccgc
    24961 caaaataatc aaacagctcc acaggatcca cagtcagcga caacaccggc agatcccggt
    25021 acattgctct tgcctccata gccacaaaat agctggcgca ggtgacatta cttgtcctgc
    25081 ctccggcatc tactgccact gcctgtaaca ccaccgcttt gtccacgtca ttaaacggcg
    25141 catagtcctg gtaggctgac actgtggaaa tggcggagta gacattggga ctgccgcaga
    25201 tattcgttat ctccaccggt gcctcataga gaattccgtt ctccggatcc ggcaccgtgc
    25261 catccagtgt ataatagata ctgcttcccg ccggtgcagc gatctctacc gtaatatcct
    25321 ccggataaaa ccctcccggc agggaaaata ccggctgatc ccctgttttg ctccgggtaa
    25381 cacgactccg gatctcgatc tgcccgcttc cttccgtctg ttcttcccgc tgcccgctgt
    25441 cctgtacctg ttgtgtcagc tgctgtctgt cattctcttg gctttgctgc agccagatca
    25501 gaaaagcgga aaatcccaca catgctaaca gtgccagcgt gattcctatt cgttcccccg
    25561 tttttttctc tgtatccatt tcttccctca ctcagtccct gtaataaata tctctggtgt
    25621 ataccttctc ccggacattc tccagatctc tgtgataacg attggccacg atcacatccg
    25681 actgccgctg aaaggcttcc agatcccgta tgaccgggct tccgaaaaaa tcatcctccg
    25741 aaagttccgg ctcgtacaac aaaatctgtg cccctttggc ccggatccgt ttcatgacgc
    25801 cctggatact ggactgacgg aaattatcgg agccggactt catgataagc cgatagattc
    25861 ctatgaccac cttttgcccc ggtacaccgc ctctaccctg acttccctga taaaatcccg
    25921 ccttctccag aatactgtcc gcgatatgat ccttgcgggt actgttggcg gacacgatgg
    25981 cctggatcag attctgtgga atgttctcat aattggccag tagctgcttc gtatccttcg
    26041 gcagacagta tcctccgtag ccaaaagagg gattgttgta ctgcatcccg atccggggat
    26101 ccaggcagac accctcgata atattgcggc tgcttaagtt ccgcatctcc gcataagtgt
    26161 ccagttcgtt aaaaaaactg acccggagcg ccagataggt attggcaaac agttttaccg
    26221 cctccgcttc cgtagggtcc atgatccgta tggggatctc ctgttttttt gcaccctcac
    26281 gcaaaagctc tgcaaaccgt cttgcatact ccgccatttc aacggacttt gtgtctgctc
    26341 ccacaatgat ccgggaagga tacagattgt catacagtgc atgcccttcc cgcaaaaatt
    26401 ccggagaaaa caggatcctg tccgtgtgat atttttccat cagagacact gtatatccca
    26461 ccggcaccgt actcttgatc acgatcaccg cgtcaggatt caccctccgt actgtctcca
    26521 ccaccgtttc cacagaaccg gtatcaaaaa agtgaattcc gggatcatag ttcgtcggtg
    26581 tggagacgat cacaagctcc gcatcccggt aagccacctc tccgtccagg gtagcctgca
    26641 aatgcagcct gccgcctgcc agatattctt tgatctcttt atcccggata ggtgatctcc
    26701 ctttttgtaa caactctacc ttctccggta tcacatccac cgccgttact tcatgattct
    26761 gggccagcag aactgccatg gacaatccca catacccggt tcccgctact gcaatcttca
    26821 tagaaagctc ccgcctttcc gtaatctgca aactttgtgg tttcctctgc ggatcggtca
    26881 tttcccgaag tcgctttatc atgaggtatt tcaggcagaa atctcttccc cattgccaac
    26941 agggcccgga aatccttccg gcctccgtaa cacaacagaa aaatgctgtt ggacacaagc
    27001 agacatacca gtgcccgctc caataattcc cggattcctt ctaccggtat ctgactgcat
    27061 acccggtcgg tcagcataca gtttaaaaat gttacgcttc catagaagac cagtgtcccc
    27121 agatagcttt gccggtcctc tccgtcaaac aacgtcgtaa acagattatg tatcagccac
    27181 ggcattccta cgatgaccgt ggcaactacc gtagacagga tcactccgta cagccccata
    27241 ggtttcacca gcagaagatt caggaccaga ttcactcctg ccgttgtcag tgtccggaac
    27301 cggtctctgt gccagatccc ggcggcatcc ttgtaggcat tgaacagctg gttgaactcg
    27361 tagataaaat aatatacaca cagacagatc acgatctgtc cgggcaaaat gcctgcctcg
    27421 ccgatccaca gtttcataaa gggctgcaac agacacagca ggcagcaggt gcaaagtcct
    27481 gcgatccagg cgatcaacag tgtaaattta cagaagtccg cataattttt cccccggctc
    27541 tcggtcagaa gactattgcc gatcccggct agagtggagg tgaaaagtac ccccaccaga
    27601 ccaatgacgg aagtaacgat gtaataataa ttctgataga ctgccagcac tgtcagtccc
    27661 aaaaatgccg aaatgaccag agtatccgcg gaattcacaa tgaccacccc cacccgggag
    27721 gtaaacaggt ccttgatctt gccccggatt ttttgcctgg tctgctcttc cacctgtcct
    27781 cttgggacat aatccggata atttttctgt gccaccagtg cggtacacag attcgtcagg
    27841 atctgtgctc ccagtgccgc cagcagatac cagtaataat tccgaaagcc caccagcagc
    27901 ccggcctgta taagatactg cagcgtccgt accgccagat ccgccttatt tgccaggtca
    27961 cttcgctgat gagctgccag cagactgttt ttatacgcaa acagccagta actgatgaca
    28021 caggctccca gatgcagcag atataataca tacaaatcca catccggcgg aatactgtcc
    28081 gttttcacca gcgagggcag aaacggcagc agcactattc cggccagaaa gatccccaga
    28141 ccgatcaggc ggtaatatct gcgatacaat gacaacagag cacagatctc gtccttattt
    28201 ccttccgcga tgggagcata catactgtag cccagtgcac tccctactcc cagctccgcc
    28261 agattcagga tctgcagtat ggaagtaaac agactgttca gccccagata ttccattccc
    28321 agataccgga tcagcagcgt gcgcatgata aaaggaacca gaatctgata ccccttcagg
    28381 agtcctccga agatcacatt tctgacggca tttcgggtcc ttttcattca cactttctcc
    28441 ttccggcttt ctggccgatc cacagatcat agggaaagct ctgtagcagt ccctccctca
    28501 gttcttccag ttcgggcaat ttcatgggtt ctgtttcctt tgcttccgca agggatgttg
    28561 ccgagcgcac ctcatatctg ccggtggcaa agtaccagtc ataatatttc agcatctctt
    28621 cctccatccc ctgccgaagg aataccgacg ggaccccata gctctccgcc agaatgatcc
    28681 catgtaacga agacgagtat accacctccg cctccagaat tttgtcgagg aaggtccggt
    28741 aatctccggt tcgtatggaa agtctgtcat ctcctgtcgt tttccgcttc atgtaatggt
    28801 cgatcaccac atattttttc tttttctccg gtcgtctgcc gggataaatc agaggcagca
    28861 ggatcgccgg gtccccgtag atctgcggac aggtatatcc gttctcccgg agcacctgtg
    28921 cggtgaccgg acctctgact gcccggatat ccagcttccg gtatcccttt tgtgtagtca
    28981 cccgtcccac atgggcgaag gaattgaccc cgctgcccca gatcaccgca tcgaaaactc
    29041 ccagtcccaa cacagaacca atggtcatca gatggcaggg ctttgccgcc tctctgtaca
    29101 gatccagtcc cagacggtgc aacatccact gcgtcaccac cggtgccagc agatccccca
    29161 gattctcatt aaaattccag tactccagat ttactttgtt cctgtctgct tttcttccgt
    29221 ccaaggtttt tcgattgcgc aatacgccga tctgttttgt cagttccaat cctctctcct
    29281 tcccctgtcc taccgccacc ggtacagacg gtccaatagc cggaatctct tatgcaggag
    29341 caactcccgc agcaagcgcc tctttcccgt ggcccggtat tcccgcaggg cattctgcag
    29401 cggcagggaa ccaccgatcc gtttcagttc cgcatatttc ctggtttcct catagccgga
    29461 atctctatag atcttatgca gatacatcag actcttcccc acgaaacagg cattgacctc
    29521 cgcatacagt tcctgctttg cctgaagcgt tcggaagcac tgccaggtgc ggtcccggaa
    29581 atacaataaa gcctcataat agttctcatg aaaacggttc accagattct ccgcttcctt
    29641 atagtgataa caatacaacg actccggaac cacacagatc cctttgctct tttccagcac
    29701 ctgcatggaa aaagccatat cctcacagat ggcaaattcc tctccgaagc ggatatcatt
    29761 ttccagaagc aattccctgc ggatcagctt attccatggt ggattgaaaa attcccgccg
    29821 gaagtccgat aaaaaccgct cctccagata ttcttttcct ccaccgcaga aagctttttc
    29881 cggaaggtta tccacacatt ctttcgtctg ttccaccaga tatgtaaaac cgcacaccga
    29941 aagatccgcg ccagatactt ttgccttttg atataaagtt gccagggtat ccgggaacag
    30001 ggtatcatca ctgtccgtaa aaaaaagata ctctccttca gccttacaga tccccagatt
    30061 tctggcactg ctgacaccgc cattttcctt gtgccagacc cggacccttg tgtcctgcgc
    30121 ccccagcctg tcacacaagg ccgaagagct atccctgctg ccgtcatcga taagcagcag
    30181 ttcccagttg gaaaagctct gccggaaaat actgtccaca cagcgctgta attctttttc
    30241 cgcgttatac accggcacta cgatactgat ctttgtctct ctcatttttc tccatcacac
    30301 tgcatattct ctgcgccacc ttgtcccagg aatactctct cagataccat tccctccgcc
    30361 gttccacttc ctcccggtat ttcggatcct gaagtacttc ccacagatcc agtgccagtt
    30421 cctccgcatc ctccccggaa ataagcctgc cggtgcatcc gtcctcaatg aactccgaca
    30481 tagcgaattt atctctgccg atacagggca gaccataagt aagtgcctct gcaaatacca
    30541 gtccataggc ttcaaaataa gacggcatac agaagacatc acacaggtta taatagtctg
    30601 tcagtttttc agaccccaga tctcccagca ggtggatctt ctgccagtcc acctcttcta
    30661 ccctgatctg cctctcccgc agagcgtccc ggatgtccga ctcctttgcc cccgccagat
    30721 acagctccgc tgaccacaga tattttttcc gcaggattgt gaaagcctgt accaccaacg
    30781 gaccaccttt tctcacaaaa ttccttccca caaacaggat acgccgcccc tcctttgatc
    30841 ccggacggat ccctgcagca tccacattga tgccgcctcc caccgcatgg accttttccc
    30901 gggggagccc gcattcccgg atcaggaact ccgaaagcca cctgctcatg gtgaataccc
    30961 cttcactttg ccggtattgc tccaattgtc tcgccgccct gcgttccagc gtcttttgcg
    31021 gcacctgatc atactcgcag taccgatagc ccaccggatc ttccctctgc agccacagaa
    31081 tcgcggaaat ggaaaggtcc tgatagagat aggatgccaa atgcgctccc gacggccagc
    31141 cgccaaactg aaacacaggc attttttcgt ctgtcctgtg ccgatgtctc cacgcagcat
    31201 atctccccga cagccgcagc cggtcccgca ggaccggccc ggggttcagt cccttcttcc
    31261 tctgcagtct ccggagtatt ttgcggtaca gaggatccga aagtgccaga tcctgcacct
    31321 gcagataagg ctccatggcc tgtcgcaggg accaggccgt tccggaccag gttctctttt
    31381 tatctttgta ccacaggcat atatacagta cttccgtcat aatcatctct tcctttccgc
    31441 atacagtgcg atctgtgtgc acccgtccgt gatgtcatag cataccgccc actgtgcccc
    31501 cggcaccacc acatcgatat caaagggaaa atcctcaaag gtcatatccg ccgttctgtc
    31561 ctcatagggc ggacacgcct taacaggcag tattatggtc tgtctgggcg tcacgatcat
    31621 cagcttccgt tcctgcgccg tatcgttggt cacgctgaaa tggatcctgc cctcttcatc
    31681 cacgaatctg ctgcctacat acaaagtatc cgtcgtataa atattctcca tggtgacccc
    31741 cgccgtgaca ttgcgatggt agaaggttcc ataacgggcc gcatccccta ccgagatatt
    31801 acacagcacc acattctcca gagaaaaacc gtcttttacc agtgtgtaga tggcataacc
    31861 gcctccgttg agaatgcagt ttgtgaaata gattcctttg gcaccgctct tctccggggc
    31921 aatcatgaga caggcattca catagctccc ccctccgggg atcgccggta cttcgaatct
    31981 gcagttgtcg aaggaaatat tctccgccgt aagagaagga tatccaaaga tctgtacccc
    32041 gtcagaatgt actgtatccc cgctgtccgg atgtgccaga tccgcaatgt agcaatctct
    32101 cagtgtcaca ttgcgatagg gattcaacgc atctccgctg ctgccaccca gacggcaccg
    32161 ggtaaacgtg gcattgcttc cctgaaaatt acgcagggtg cagtcttcga acacatagga
    32221 tatccgggca tcctcccgcc cggtagtgac tgcatcgaat ttacagttcc ggaaagtcac
    32281 catggtctcc acggcaattt tgccctcatt gaccatcaac agtgcatacc gggaaaaatc
    32341 catattccgg atctctatct cattccccgc ctgtctgttg ctgtagacca gatccagtgc
    32401 cagctgtcct ccagctccgg tcttatactg tattccaccg taaatccccg gttccgggat
    32461 ctccgtcagc tctgtatctg tatccacacc ggtattgaaa tggtccggga tctctctggg
    32521 tgccgcctgc gcccgtatcc ccagactcat actcagaact attgcaaaca gccaaaaact
    32581 ttttcgcatt ttcgtttcct tttttcgttt cttctctcta tttaccttat gtatctgtgc
    32641 accgctttgt gttgcaaatc cgacatattt tcccattttt cccgggagat cacctggaac
    32701 agtgcgatgg gaagatacgc atacttgatg gtctgcagcg tatccatcca ccacagattt
    32761 aagaagtacc cgcagaccgc aagcgccaga cataacattg ttctgtcctt cttttgaatc
    32821 ccgcatcgca ccatctcccc caccgcctgc aggatcaaca gtccgtagac caccagtccc
    32881 ggatacccgt agcggatata attggccaca tagaaattgt caatggagtg gatcgtggcc
    32941 cctcccggaa tataccagga gaaatgatat ccggagccct gccccaacca ggggttcagc
    33001 cagtccaccg tgaaaatatc cagcagggca tccctgtagg aagcgctgtt ggcaagggag
    33061 gtgctgtcca ccccgtaacg gatggcatag gaggttccaa acatctcatc caccactgta
    33121 ctaagctgca acagaatata atgtcccggc ggagaattgg gaaataaaaa tgcaagaaac
    33181 agaatcaaaa gcccggccgg tactatggta aacaacagtt tcttcctctg ctctcccggt
    33241 gccagcacgg tcaacagcaa aagctccagt cccatgaccg ccagcgtgga acgggatccc
    33301 gtaagaaata cattaaccac caccaatagc aggagaggca gatgccgaaa cagcgtgaag
    33361 caatgtttct cctcatcaat gcacaataat ggtaacccgg agatcaggat cagcccatac
    33421 gccagagaat gattggccgg ccccatgatc cggtaggaac cggagcggac catgcctccg
    33481 gtataaagtc ccctgatggt ctccagatag gcaaagggtg tctttctcat gaccgcctcc
    33541 acgagtccca gcacacacag aaacaaaagc atccgccgga aaagcaccag aaatccatca
    33601 cttcccagat actcccgcag tacgtagatc accagaaaag ctcccaataa ttcaatgaac
    33661 ggataaagaa aagtacctat gtgaaaacgc agtaccgccg tatagaaaca aattcccaga
    33721 tatcctgcca taaaaggcca caatctgcag cttctcaccc cttcccagaa catctttttc
    33781 cgggattctt tgctcagcag aaagagacac agaatcgtta tgagcattcg ctgtgccgtc
    33841 agatcaaagc ccggcagccg gattccgaaa tactgtggta aaatataata ggccacaaga
    33901 aaagaaaccg acaaaaactg tccaagactc cgctccccac aattttcaaa atagcggttg
    33961 ctgctctgcc agccctgccc cgttgtcttc gttttttcca ccagatatct gctcattgcc
    34021 tcatcctctg actgccatgg caaattccac cggcagagat tttttgctgt gataacaatc
    34081 ccggtatttc cataaaagcg gcacaatatt ccacaatttt cgctgttcca tcagctcgat
    34141 ccgaagggtg gtggcccgga tattgcgtct taacagttcc ttcgcttcct ccggcattcc
    34201 aaagtcctcg gcgaaccgca gagtggtttc atgacttttc tgcaattccc ggaaaaaagc
    34261 gatccgtctg tccagatccc gcatcttgcg tttggataca ttacccgagt gcattcgccg
    34321 ccgtaacgta acactgtgca gaatgtaaca gccgtccatg cacagggaca acttccaggc
    34381 atattcatca tgcgcccacc cgggatacca gtacttcttc gtctcttcca ggaactctct
    34441 gcgcagacac atggtacagc cctcactgcc gatgaaaata tgggccggac tccatgcaac
    34501 atgctccatg cttccgtccc cccggaagct tttcaatacg ctccggctga cagaaggagc
    34561 atcgggatcc actgtaaacg gtacgaattc cgatcccagc accagtatct ctctccgttt
    34621 ttccatctcg ctcaccatat cttccacccg gtccggttcc cagatatcgt cctgatcgca
    34681 gaaaaagaca taatcaccgt ccgtctcttc caccgctgcc cggaaattct ccgcataccc
    34741 cagtcgtttt ccgttctgtg tgatcctcca tgccggtgcc aactcgtaag tctccagata
    34801 ttcccgtacg atctccagcg tctgatccga ggaaccgtca tcccgcagga tcacttgatc
    34861 caccggaagg ctctgaagcc ggatggaatc cagctgttct gtgataaatc tggccccgtt
    34921 ataagtggcc attgctaccg atatccgcat atttttcccc ttttctctgt ccgccatttt
    34981 ctatccaggt aaccctcccg gcctgcatct gccgatggtt cccccacaga tcccgcacaa
    35041 tgccggtgtg gcgatccgca gataacgccc tttcagtcca ttgcccagat aggggttctt
    35101 ccgatactcc ggaaaatttt cccgaaggaa cacaaaagtc tcttcccgca gagatttccg
    35161 caacttccgg tcccgcactc tggagatccg ccccatggaa ctgcacaaca ggatcctcgt
    35221 acagaaatat tccagttctt tcccataaat atccatcagt tctttttccc ggtaaaaccg
    35281 acaggcatcc tgcaataccg gaaaaatatt tcccacccgt ctgcttttat tgtcattcat
    35341 ggtagaatcc cctctgtccc ggtgatagac atagactccc agcattttcg cctgtttttt
    35401 cacaaaggga cgtaacttca ggtagaaaga ggtgtcctca tagatatacc cctccgggaa
    35461 ccatatatcg ttgtccgtaa gcagtctcct gcggatcagc ttgttccagg gagccgccga
    35521 accgtaagag cgcttctctc tgccatcttc ctccagataa tgaaaatcac attccaccag
    35581 atccagttct tcggaaccgg ccctggtgta catggccgcg tacatacgcc tgtccacata
    35641 atcatccgaa tccacgaatc ccacatattc tcccgtggca gccgcaagtc ctacatttct
    35701 tgcctttccc tgccctgcgt tcttctgggt gatgacccgg atgtgttcgg gatccctggc
    35761 acgatatctt tccagaatct tgggagaagc atccgtggaa ccgtcatcca cgaagatcag
    35821 ttccagtgaa gacaacgtct gctctgtcag ggaacgaatg cattcctcca gatatttttc
    35881 cgtattgtat accggaacga tcacgctgac tgccggcata agatttcctc cacactatgt
    35941 aatagaaatt ccggaaaata cagactgatc tccccgtgtt ccttgtaatg tgccacgtcc
    36001 tcgctgcagc ggtatccctt tttcgccaga tcccggcgca gatagcggat atgttcctca
    36061 taggtgtgtt cgctgtcgga ataatgcaga tagatcctct gggagggcgc ataacggttg
    36121 gctgccacct ttttcggaag atactcattg aggaattcga ccttttcctt acaaacctgt
    36181 ccggcaatat gcttcaacgc ctcaatattc ccggtgtcca gcaggctttg tcccagaaaa
    36241 tactgggggc cacccaccac catatagctt ccgggatagt ccagtccgaa attcagtgcc
    36301 gtgtaaccgc ccttgctgga accgcagaag atcaggcgtt ttgctccggt ctgctcccgt
    36361 acccggtcga tcagttccct tacaacgacc gcctcctcga atttcatatt gctgcccaga
    36421 taaaaggaac ctctgtgatc tgacgcaaaa tcatccaaca gaaacaactt gttacaggga
    36481 acttccttta acgtccggac ataattgtat ctggcccgga tcccttttct ggtgcaggag
    36541 ctgaaaacga tgatcagatc ccgggaatcc acccggtcca gcatatattt taatgtcact
    36601 ccccgggagc gatatttttt ctgatcccgc aaaaggcgca gcttcgtcat catatactct
    36661 cccagttttt tccctttttt acgaaatttt tgaatgtttt ccatggtttt ccccctcttc
    36721 cgcaattaaa aaattctcaa tatccttaag gatcctccgg gtattggaat ggtatccggc
    36781 aaagtccctg ctgccggtat cccggatgca atcccccagt ttctcactgt cataacaggc
    36841 acagatcagg ttcattccgt cgaattcccg aagcagctgt acctgatgat cgtccacatg
    36901 ctccccgtag ctggcaagtc tcggcactgc gatcactttt ttcccttgtt ttacggcgcc
    36961 gatgatcaca ccggttccac catgggtaat gaccatccgg cattcccgca gcttcccggt
    37021 gaagccttct ctgtccagaa acctgcagta ttcataatgc tttggctgat aatcgctgac
    37081 tccgatctgg gcaaacacct cctctcttat catgccttcc tcgatcagca gatctatctt
    37141 ttgtaacaat cgattgaact ggaacttctg agatccggtc gttacaaata tcatcctaat
    37201 aaattccccc caggcagatc gcatccggat agatttcctt catctgtgtc cattggacat
    37261 aaaaacgatc cgcataatga tataacagtt ttcccgtcag tgtggccgaa gtcactttgg
    37321 caaatgactc gatgtagatc agcttcctgc cggagagctt ggcttccatg cacagcggga
    37381 tcatggccag cactccggtg cagaccacca catccggctg ttctttatgc aacagccgca
    37441 acgaatccac ggtatttttc aacagataaa aaaggcatct ccactgtctc cggttcacct
    37501 gctgcagata gtaacatttc accggtgtct tcacctcata gcctgttttc tccgttacta
    37561 caaaactttc gtatttttcc attagcggtt ttaacatcat cagttgttca aagtggccgc
    37621 ccgacgatgc ggcaaaacat attttcataa agacctccct ttttttcaaa ctgcttcccc
    37681 attttgctgt ttatattcct gccagtattg ctgcagtctg tagttacagt ccttagattt
    37741 tggcggcagg ccgttgaaca ggatgccgca gatatccgca tccacctgct ctaacagtgt
    37801 caccgccgcc ttttcccggc tcatatggct gacgccggcc cgcaccacca gaagataggc
    37861 ggtgacttca tgggacaata gcagggcatc cgtcacaatt cctaccggcg gcagatcaat
    37921 aatgatgcag tcgtaccggc tacgcaacgt cgtaagcagc tccttcatcc gctctccgcc
    37981 caacagttcg gaaggattcg gtggattttc tcctcccacc agcacatcca gattctgccg
    38041 gtattccacg atctgcggtg tctcccgcaa tcccgccaga tactggctca gtcccagtct
    38101 gccccggagc tttagcagag atgccaccga catattccgc atgtctccgt cgatgagcaa
    38161 tacttttttg cccagctgtg cgtaactgat ggcgatgttg acactgttca aagtcttgcc
    38221 ttcatcctta tccgcactgg tgacggcata gaccggacaa cgttctcctt ttcccgtgaa
    38281 cagcaggttt gcccggatgg tattatagga ttccctcacg gcaaaggagc tgttctcccc
    38341 caataccaac acgggcttac tcttccggtc cggcgcctgg atctgcggaa tatctcccag
    38401 caccggaatc cggaatgcca gcttcagttc gtaccgcctg cggatcgtgg tatccatcag
    38461 tcctgccagc agaaacaata caaaggatgc cccaaagcat ccggcaaagc ccaccagtgc
    38521 ataattgacc agactggtag aactgtaggg tgcttccggc agtgtggcat aatcgatcac
    38581 ctcaatgcca ccggacttca cgatccggcc gatctccttc ggagccaccc tgccgatagc
    38641 attggccagc tccatggccc ggtaaggatc gctgtcataa gtgtagacat agaacactgc
    38701 cgtgttttcc accacactgg taccgattct tcccgacaaa gcttccgttc cgtaatccag
    38761 cccggcttcc tccagcacct tctgaaggat cgtgttgctc ttcaggatta atatgtaact
    38821 ctgtaccaga ttcttggcct gggtaaattc ggaatttgag atattgaccg aagaatcata
    38881 cacatagtcc ggatcactgt atacatagaa ggatacctcc gcccggtaaa gtgtactgtt
    38941 agtcagatat gcataagcgc ctcccgccat tcctcccagt acccccagga tcataatgta
    39001 tgcaatcctt ttttgcagga tccacaggat cttctccaga tgaaaattat tcaagatcgt
    39061 ttccaacatt gtcctctcct tttcgacaaa ttctgctttt ccctaaaatg aaaatatcca
    39121 ccagggaatt ccctgatgga tatcttacgc cggacattgg cctgtccgtt ttcttcttat
    39181 gtaaaccgta taaataatac gttccatata tgcagccacg cctccgctca tacactccgg
    39241 aagcacttcc gtatataaag acggcgctaa gaagcggaat ggctcaatat ttcttttctt
    39301 ttttttaagt gcctgttatg atataatgag cgcaagagag ccaacatgct tgcatgattg
    39361 gcgaacggcg aagtgatttc ccgatgtaca cctcaccacc agcgcaatca aaggagtgaa
    39421 cctatatgat acagaaaaag aaattcgtaa aaaccttccg taacaccaaa accgtgcagt
    39481 taattcttct tctgctgttg gaaaccgcct atgttctggt cctgactctg aaccctgcac
    39541 tgggcacaaa gcttttcgaa gaccggactc tgtttatctt atgcacactt tcctggatcc
    39601 ttgcattatt taatctgata tggttattat acgatttcta taaattacgc acttttgccg
    39661 aagaaagcca tgctctgaac cgtgcggcct tcctggatca tctgaccggt atccccaacc
    39721 ggcacggtct ggacgtggtc ttcgagacct atgactctcc ggaatctctg gctcatgtag
    39781 cctgctatat ggtgaccatc accaacctga aagagatcaa ccagaccatg ggccatgtcg
    39841 taggtgacca gatgttaaaa gacttctccg gtattctgga aagcgttggt gatgctttcg
    39901 gtgtggtggg ccgtaacgga ggcaacgaat tcctgatgat cgtcaaccac ggctctcatg
    39961 acaccatgga atatttcatc gaaagtctga accagcagtt aaaagagtac aacgaggagc
    40021 acagtaacgc tcccattcag atccaatacg catatatttt aaatgaagag gcgcatgccg
    40081 aatatttttc caccctgctc acagagacct acaacaagct gcacccctaa ggaacccgtt
    40141 aacacaacag tgagacgaac gcatgaaaga cttgaacatt ccttacattg ccggtcttgt
    40201 gatccgtgcc cagcatagcg actcggatgc cttcgcagaa ttgtatgctc ttacctataa
    40261 taaagtatac aactacacca ggcattatct gagagatgac tttctcgctc aggatgccat
    40321 gcaggaggtt tacatactgg ctttgaaaaa catcggaaaa ctgaatgatc ccaccgtttt
    40381 tatcgcctgg ctgaaccgca tctgtttcca tgtctgttat gacatgaccc agaagaatcg
    40441 tccgcagaat gactgtcagg tcttaagtca ggaaattctg gatattcttc aggaccctca
    40501 tctggatgcc aatccggaac tgcactacca gcagaaggat gagtattccc gcctgacgga
    40561 agctttagag cagttaccct ttaatgaaaa acaggtgctg atcatgcgtt attataacga
    40621 catgaagcta gaagagattg ctgccgccat ggagatcagc cgcagcagcg tcaaacgcta
    40681 cattgcctcc ggacaggaac atttgaaaga aagtatgaaa ggataacaga atgatgtttt
    40741 ttcataaaaa aaacagatat gaactggaca tgacaactgc caacaatgct ttgcagaata
    40801 tcctgtccag ttgcaaccag ccggtcaata cgatcccttt tgacaagctg gtgcttcgca
    40861 aaaaagtgaa tgccgcctcc tacaaccgcc tgatcgttgc cacgacactg atcttcgtgc
    40921 tgaccttttt gtcgccgctc gccatcgttc ctctctctga gatgactgaa aaactgttgg
    40981 ctcccacacc tgcggtgctt acactggatt atgtggaaaa taatatttta agtctgaagt
    41041 tcaccggtga caacattctt tacgaggagg cttttatgga gactgtgtcc ggggagatca
    41101 tcgaaccgct ctctgtggat tcctccaagg gtgtgatcaa ttttccgttt ttgtccgagg
    41161 aagccaatat ttatgtcccc gtcaaaaacg gtgagactct ccacctgctt tttactcccg
    41221 ataatgtaac gggcctggaa caataacaac aggatgtaac gatttttatg cggatgccaa
    41281 aattatctgc gcgactgcgc attttgctaa ttacatattc catgacaatc ggcttatgtg
    41341 cctgcggatc cggctctgac aatctgcctg tggatgcccc cgtcgcagac acttacgacg
    41401 aaccatcaca atcctccgca gttccttctt ctgcggatac gaatatcagc tcttcggatg
    41461 cctcttctcc tgaaacggaa gccgtaactg acgcagagcc tcttcgggat gccacgcctg
    41521 tctgtcttgt gccccgggcc gacggaacag caacagcttc caatgatgtg gctgtcattg
    41581 actattctca tatgtctaac ggctatgtct gtgccaatta caccggcacc tgtcccaagg
    41641 tcaaactgcg cattaccggg ccggataccg ttgtctatac ttacgatctg cacggtggcg
    41701 gctatgagac tttccccttg tcttccggag acggatatta tgacgtgacg atctatgaaa
    41761 atatctccgg taccaattat gccacctgcc tgtatgcaga cctggatgta cagattacgg
    41821 atgcattcag cccttttctc taccccaacc agtacgtgaa ttttactgcg gacagcaaag
    41881 tggtagccaa gggacaggaa cttgccgagg gtgcttcctc ggatctggaa gtgatcaccc
    41941 ggatctatga ttatattacc cagaatatta cttacgacta cggcaaagcc tccgatcctc
    42001 ctacgggcta tacctccgat gtggatgcca ttcttgcttc cggcaccggt atctgtctgg
    42061 attatgccgc ggtaatggca agcatgctgc gaagccagcg gatcccgacc cggctggagg
    42121 tcggctatgc ccaggatgcc taccatgcct ggatcagtgt ctacaccgct gataccggat
    42181 ggctgaacgg catcatagaa tttgacggca atgtctggac cctggtggat cctacgttcg
    42241 gtgccaacac ggatgacaag actctgaaaa agttcatcgg tgacggcagt aattatatcc
    42301 tgcagaaaat gtattagtga aagggaacaa aacgccatga agaccacaga gaaaaaacta
    42361 ctttctaatg aagagatcgc ttctttttgc agtcaggctg ccatgctgtt tcaggcaggc
    42421 attccgcccg tggagggcat ggccatcctg caaagtgatg cccagtctcc cgaaggcaag
    42481 gcgatcttcg aggagatcct taccgtatgc cgacagggag aaagctttca caaagcactg
    42541 gaagccacca aggtcttccc cgattactgc ctgcatatga tcgctctggg tgaagaatcc
    42601 ggtaatctcg atgtgtgtat gagctctctg gcggattatt atgaaaaaga ggatgccatc
    42661 gcaggcagta tccgggatgc tgtaacatat ccctttatca tgatcgccat gatggccgct
    42721 gtcatcgtgg tactggtcag ccgcgtcatg ccgatcttcg agcaggtcta tatagagctg
    42781 ggcagtgaga tgaccgggtt tgctgcatca cttttgcgtc tcggcaacca tctgaaccgg
    42841 tattccttta ttttcgtaag tattctgtgc atattgttgt tactgtacct ttttgctacc
    42901 agaacccaga ccggcaaacg tgtcaccgcc cggttcctga actggttccc cctcaccaga
    42961 cgtttttatg aaagtgtggc ctgcgaacgt tttgccagcg gtatggcact gaccttaagc
    43021 agcggtatgg atacctattc cagcctggat atggtggccg ctctggtagg caacgagaaa
    43081 atgaaacaga agatcctgag ctgtaaggaa gccattaatg ccggtgccaa ttttgccgag
    43141 gctctgaccg gtgccggcat tttcaaccac ctgtattccc agatggtatc tgtcggtttc
    43201 cgcagcggta acgtagatgt ggttctcaaa aaaatagcgg atcgttatga ggaaaatacc
    43261 aaccgcagac tacaatccat cattgccatt ctggagccga cccttgtcat tattctgtcg
    43321 gtgatcgtgg gcttgatcct gttatcggtc atcctgcctc ttatgggtat tatgaccagc
    43381 atcggatagg agtacgatac tatgaaccgt tttgagcaaa aaagtatgtc cctcgggcgt
    43441 aagctgatct atttacttcc cattctggcc tttgtgatat tgtttgttct ttttctgcgg
    43501 ggcatcggct ccgtcggtga atctactctg agcaagcagc aggaaagtct ggaaaccgca
    43561 ttggaacgca gcatttccca gtgctatgcc gtagaaggct gttatcctcc ttctctggaa
    43621 tatctggagc agcattacgg actgttgtat gacgaggaca gcttcttcat tgactatgaa
    43681 tattatggca gcaaccttct tccggaagtc actgtacttc gccggaccgg tgctcactaa
    43741 aggagtttct gacatatgaa caatagaaat catcagaagc aggaaaaaca ttttattgtg
    43801 gatatcttgt tcgttctggc gttgtttggt gtattcgctg tctccgcact tgcccttgtt
    43861 accatcggag cagatgtata ccagcacact gtagaagata tgggcgtcaa ttacgaaagc
    43921 cggacggcgg tgtcttatat catggaaaag gtgcggcaga acgataccgc tgacagtatt
    43981 ttcctgaccg atctggaaaa tgttccggcg ctctgcatgc tttccgaaat tgacgaagaa
    44041 acctactgta cttacctcta tctgtatgac ggacatttaa aagaactttt tatgcgggaa
    44101 ggagcttctc tcggcggaca ggtattgcct gccggtacgg atattatgga cttgcaggag
    44161 ttctccttat cctatgcgtc ggacgatctg atccggatat ccctgagaac cgcctccgga
    44221 gaggaccata ctttttatat ccacgtgcac tgtaatacta ccacagaata gaaaccgaac
    44281 agaaaggatt ccgaacttat tatgaaacat tccagatcca gtttattttt aatggaaatg
    44341 atcatagcga tcctgttttt ttcactggcc agtgccgtct gtatccagtt gtttgcccgc
    44401 tcacatcttt tgagcacaca gacggtaaac cagaatcatg ctgtcattca ggcacagaat
    44461 ctggcagagt cctatctctc tctggagggc aatgtggctg ccatgcagga tttgttctcc
    44521 ccatcggagc tgaccgatga aaacactctc cgccttgcct ttgacagcag ctggaatctg
    44581 tgcactgccg aagataacgc cggcttttat gcagaactaa aaagcactcc ggcagaagag
    44641 accggaatca tgaaaggcga gatcaccgtc tatgatgtat ctacaccgga atctcccatt
    44701 tacacactct ctgtcatgca tcacaccgca gaaaggaggg aatcccatga ataattcctc
    44761 cagaaaaaaa caattcggca tgaatatcgg atccgcctcg atccttctgg tattcgtgat
    44821 cctctgcctg gtgtcttttg ccgtactgtc catcgtcagt gccaacgctg atagcaaact
    44881 cagcgcccgt gtgctggaac gtaccaccgc ttattatgcc gcctgcaatc aggcagaaca
    44941 atccctggca ggtatggata agacgttgca acgcatgtat gcatccagtg acagcgaaga
    45001 agcctatttt gtggcggtag gtcacggaaa atcttacgtg attccaatct ccgacttaca
    45061 gaccctgcag gtgaccattg agatcctcta tccggaatct gatgaagata ccttttaccg
    45121 gatcactgcc tggcaggttc tgaatacaga agaattgcaa taattgttcc gattctaaaa
    45181 ggctccccgg gaaaatcccg gagagccttt cttaataaag tctttttatt ttactgatta
    45241 tcagcgtctg ttcccacttc cacagtttct gcttctgtgt tcacatcttc attcagttct
    45301 gcaccttctg catccagttc gcttccgtca ctcaggttca gttctgcatc gtcaccgtac
    45361 atatcaccac cgaagtcaat ggtctctacg gcatcggtgc tcagtctggt attgcgatat
    45421 ctcttcatac cggttcctac aggaatcagc ttaccgatga taacgttctc cttcagaccg
    45481 atcagattat ctaccttacc cttgatggca gcttctgtca ggaccttggt ggtctcctgg
    45541 aaggatgctg cagacaggaa ggactcggtt gccagtgctg ccttggtaat acccaggatg
    45601 atacgcttac cttctgcagg ctccttgcct tcagcggtca gcttctcgtt catttcttcg
    45661 aaatccagaa catctaccag agttccgggc aggaactcgg tatcgccgtt attctcgata
    45721 cgaaccttct tcagcatctg acgtaccagg acttcaatat gcttatctgc gatatctaca
    45781 ccctgcagac gatatacacg ctgtacttca cgaagcatgt aatcctgtac ggcacgaata
    45841 cccttgatct tcaacagatc gtgagggttt acactacctt cggtcagttc atcaccggcc
    45901 tccagtacct ggccgtcgat aaccttgata cgggaaccgt aaggaatcag atatgccttg
    45961 gattcaccgg tctcttcgtt ggtaattacc acttcacgct tcttcttggt atcacggatc
    46021 tcagccttgc cgccaaattc agcaatgatt gcaagtccct taggctttct ggcctcgaac
    46081 aactcctcga cacggggaag accctgagtg atatcgtcac ctgcgacacc accactgtgg
    46141 aaggttctca tggtcagctg ggtaccgggc tcaccgatag actgtgcagc aatgatacca
    46201 actgcttcac ctacctgtac ggcttcaccg gttgccatgt tggcaccgta acattttgca
    46261 cagataccaa cgtgtgaacg gcaggtcagg atcgtacgga tcttcacttc ctcaatgggc
    46321 tcaccctgtg cattgacacc aacacttaag atcttggcag cacggctggg ggtgatcatg
    46381 tggttgtgct taacgatcat attgccgtct ttatcataaa tatcctcgca ggaatatctt
    46441 cctgtgattc tgtctttcag gccttcaatg gtctctttac catccatgaa tgccttgact
    46501 accataccag ggatctcagc ctgaccctca cagcagtcta cttcacggat agaaagctcc
    46561 tgagatacgt caaccattcg tctggtcaga taaccggagt ctgcggtacg cagagcggta
    46621 tcggacatac cttttcgtgc accatgtgcg gacatgaagt attccagtac gtccagacct
    46681 tcacggaagt ttgacttaat gggcagctcg atggttctac ctgtagtatc agccatcagt
    46741 ccacgcatac ctgccagctg tttgatctgc tgactggaac cacgcgcacc ggagtctgcc
    46801 atcatgaaaa tgttgttgta tttatccaga ccggacagca gtacgtcggt cagctcttta
    46861 tcggtctcgt tccaggtctc tacaacggca cgataacgct cttcctcggt gatcagacca
    46921 cgtctgaagt tgcttgcgat cttgtctacg gtagcctgtg cagctgccag catttcaccc
    46981 ttcttctcag ggacggtcat atcggaaatg gatacggtca tagcagctct ggtagagtac
    47041 ttgtaaccga tagccttaac atcatccagt acctctgcgg tcttggatgc tccatgggta
    47101 ttgatgacct tctccaggat ctgcttcaac tgcttcttgc ctacgtggaa gtctacttcc
    47161 agcttcagga agttctcggg atcgcttctg tctacgaatc ccagatcctg aggcaggatc
    47221 tcattgaaga tgaaacgtcc cagagtggac tctaccgtac cggtaatggt ctcgccctct
    47281 gcatttacct tgtgcatacg cacatggatg cgggaatgta aggtcagcgc ctggttctca
    47341 tatgccagga ttgcttcgtt tacgctcttg aaatacttac cttcgccgat cgcaccgggt
    47401 ctctcctggg tcagatagta gataccaagt accatatcct gggaaggaac ggctacgggt
    47461 ccaccatcgg aaggcttcag taagttgttg ggagacagca gcaggaaacg acactctgcc
    47521 tgtgcctcct gggacagagg aagatgtaca gccatctggt ctccatcgaa gtccgcgttg
    47581 aatgcggtac ataccagggg atgcagcttg attgccttac cttctaccag gatcggctca
    47641 aatgcctgaa tgcccagtct gtgcagtgtc ggcgcacggt tcagcataac aggatgttcc
    47701 ttgataacct cttccagcac atcccatacc tgtgtatcca gcttttctac cagcttcttc
    47761 gcattcttga tgttctggct gattcccttg gctaccagtt ctttcattac gaaaggcttg
    47821 aacagttcga ttgccatttc cttcggcaga ccacactgat agatcttcag ttccggtcca
    47881 acgacgataa cggaacgtcc ggagtaatct acacgcttac ctaacaggtt ctgacggaaa
    47941 cgaccggtct taccctttaa catatcggac agagacttca gcgctctgtt accggggccg
    48001 gtaacaggac gtccgcgtct gccattatcg atcagagcat ctaccgcttc ctgcagcatt
    48061 ctcttctcgt tacgaacaat gatatcagga gcaccaagct ctaacagtct cttcagacgg
    48121 ttattacggt tgatgatcct tctgtacaga tcattcagat cggaggtagc aaaacgtccg
    48181 ccatccagct gtaccatagg acgcagatcg ggaggaatga caggaaccac atccatgatc
    48241 atccactccg gcttattacc ggactcacgg aaagcttcta caacttccag tctcttaatg
    48301 atacgtgcac gcttctgacc ggaggactcc ttcagaccgg tcttcagctc tactgcctct
    48361 ttttccaggt caatggcctg cagcagctct ttgatagcct ctgcacccat gcctacacgg
    48421 aacttgttgc cccaggtctc tcttgcatcc tgatattcct tctcggacag cactgccttg
    48481 tattcaaggt tcgtggttcc cggatccaga acgatatagg aagcgaagta cagaaccttc
    48541 tccaggattc tgggagacag atccaggatc aggcccatac ggctggggat acctttgaaa
    48601 taccagatat gggatacagg agctgcaagc tcgatgtgtc ccatacgctc tctacgtacg
    48661 ctggctttgg taacttctac gccacaacga tcacagacta cgcccttata tctgatcttt
    48721 ttatatttac cacaatgaca ttcccagtct ttgctgggtc cgaagattct ctcacagaac
    48781 agaccgtctc tttcgggctt cagtgttcta tagttgatcg tctcgggctt taatacctcg
    48841 ccatgggacc actcccggat tttctccggg gaagctaatc caatcttgat ggcatnnnnn
    48901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccatg ggaccactcc cggattttct
    49021 ccggggaagc taatccaatc ttgatggcat caaatgtcat aggctgatag gttgtttcat
    49081 tttttgtttc tgacattaat ggcactccct tctatacaaa gcattaaaat tcatcatcac
    49141 tgccgtcaaa gttatcatcg gtgtaatcat cgtcaacgta atcgtcgtcc tcagagttca
    49201 ccagctcacc attgtcatca aattcctgtg actgatagcc catctcgcca agggagctct
    49261 tgtcgtaatc gtcatcgtac ttacgatcac cttccatctc ataacggtaa tcggtatcgc
    49321 cgtaatcgac agtctccatg atctcaacct cggtctgatc ctcacgcagc actctgacat
    49381 ccagacccag tgcctgcagt tcctttaaca gaaccttgaa ggactcggga ataccgggct
    49441 cagggatatt gtctcccttg atgattgctt cgtaagtctt cacacgtccg atcacatcat
    49501 cggacttcac agtcagaatc tcctgcaggg tgtaggatgc gccatacgcc tccagagccc
    49561 atacttccat ctctccgaaa cgctggccgc cgaactgggc tttaccaccc agaggctgct
    49621 gtgttaccag ggagtacggt ccggtagaac gcgcatggat cttatcatct accagatgat
    49681 gcagtttcag gtaatgcatg tgtccgatcg ttacagggcc atcaaagtat tcaccggttc
    49741 taccgtcacg aagacgcacc ttaccgtctc tggaaatggg aacacccttc catactcctc
    49801 tgtgatctct gttctcagac agatactgca taacctcagg aagaagctct tcaccatgct
    49861 tgctggtaaa ggtctctcct gcccagggct taccgtctgc ggtagtttcc tgtccctttt
    49921 ctttccactc tgcactttct gcatcgtcga aaggaaggtt tacataatcg ttggcaagat
    49981 ccaacgtatc cataatatct ttctcgttcg caccatcgaa gacaggtgtt gccacattga
    50041 atcccagagc cttggcagcc agggaaaggt ggatctccag gacctgtccg atattcatac
    50101 gggaaggcac gcccagaggg ttcagcacga tgtccaaagg acgtccgttg ggcagataag
    50161 gcatatcttc tacaggaagc acgcgggaaa cgacaccctt gttaccatga cgacctgcca
    50221 tcttatcgcc tacggagatt tttctcttct gtgcgatata aatgcggact gcctgattta
    50281 ctccgggtgc cagctcatcg ctgttctcac gggtaaatac cttggcatcc actacaatac
    50341 catattcgcc atgaggtacc tttaaggagg tatctcttac ttctcttgcc ttctcaccga
    50401 agatggcacg cagcagtctc tcttctgcgg tcagttcagt ctctccctta ggagtaacct
    50461 tacctaccag aatatcaccg gcacgaacct ctgcgccgat acgaatgata cctctgtcat
    50521 ccagatcctt cagtgcatcg tcaccgacac cgggaacatc acgggtgatc tcttccggtc
    50581 ccagcttggt gtcacgggct tctgcctcat actcctcgat atgaacggaa gtataaacat
    50641 cgtcacgtac cagtctctca cttaacagaa ccgcatcctc gtagttgtaa ccttcccagg
    50701 tcatgaaacc gatcagaggg ttcttaccca gtgccagctc accctgagag gtggaaggac
    50761 cgtctgcaat gacctgacct gccgctacat ggtcaccctt gaatacgata ggcttctggt
    50821 tgtagcagtt ggactggtta ctgcgggaga acttggtcag atggatctct gccttttctc
    50881 cgtcatcata agcaatgcga atgatcttgg aagatacata ggtaatggtt ccgggcttct
    50941 tggccactac gcagacaccg gagtcaaccg ctgtctttac ttccataccg gtaccaacca
    51001 caggagcctc agtgcttaac agaggcacag cctgacgctg catgttggat cccatcagcg
    51061 cacggttcgc atcatcgttc tgcaggaaag gaatcagtgc agtagccaca gagaatacca
    51121 ttctcgggga tacatccatg taatcaaaca tttccttagg atattcctgt gtctcatcca
    51181 gataacgtcc ggaaacagat tttctcacga aatgtccctc tgcatccagt gcttcgtttg
    51241 cctgtgccac atggtaatta tcctcttcgt ccgcagtcat gtagactact tcatcggtaa
    51301 cgcgggggtt cttaggatcg gacttgtcga tcttgcgata aggagcctct acaaaaccat
    51361 attcattaat tcttgcatag cttgcaagag agttgatcag accgatgtta ggaccttcgg
    51421 gtgtctcgat agggcacatt cttccataat gggagtaatg tacatctcga acctcgaatc
    51481 cggcacggtc tctggacaga ccgccggggc ccagtgcaga cagacgtctc ttgtgggtca
    51541 gctcacccag agggttattc tgatccatga actgagacag ctgggaagat ccgaagaact
    51601 ccttcactgc agcggttaca ggcttgatat tgatcagaga ctggggagaa atatcctcag
    51661 catcatgtgt tgtcatacgc tcacgtacca ctctctccat tctggacaga ccgatacgat
    51721 actggttctg cagcaactca cctactgcgc ggatacgtct gttgcccaga tggtcgatat
    51781 catcatcgtt gcccaggcca tactccagat gcatattata gttgatggaa gccaggatat
    51841 cttccttggt aatatgcttg ggtaccagtt catgtacgtt cttcttgatt gcttctgcca
    51901 gcttctcggg atcatcgccg aactcttcca ggatctgtgc cagaaccgga tagtatacca
    51961 gttcatggat accgaaatcc ttgggatcgc aatctacata gtgggtcaga tctaccatca
    52021 gattagacag tacttttaca gttctctctt cggtctggat atatacgaag ggaactgcgc
    52081 tgttctggat ggtatctgct agctctgcgg tcaccttgtc tcctgcggat gcgatcactt
    52141 caccggtgga aggatccact acatctgcag ccaggatctg atgacggata cgattcttca
    52201 gagctaactt cttattgaat ttatatctgc cgactttggc taaatcatat cttctggggt
    52261 cgaagaacat agcattgatc aggctctcag cgctctcaac gcttaaaggc tcaccgggac
    52321 ggatcttttt gtataactct aacagaccct cctgataatt ctcagcagtg tccttggtaa
    52381 agcttgcctc gatcttgggc tcgtcaccga agagttcacg gatctcatca ttggtaccga
    52441 tacccagggc tctgatcagt acggtaatgg gaaccttacg ggttctgtcc acacgtacat
    52501 agaagacatc gttagagtcg gtctcatact ccagccatgc accacggttc gggatcacgg
    52561 tacaggagaa gagcttctta ccgatcttat catgtccgat tccgtagtag ataccgggag
    52621 aacgaaccaa ctggctgacg ataacacgtt ctgcgccatt gattacgaaa gtaccggtct
    52681 cagtcatcag cggaagatca cccatgaaga tctcatgctc actgatttct tccttctcct
    52741 tgttataaag gcgaacctta accttaagag gtgctgcaaa ggtagcatct ctctctttac
    52801 actcttcgat agaatacttc acgtcgtccc tgcagagttc aaaatccaca aattccagac
    52861 tcaaggaacc gctgtagtca gcgatcggag agatgtcgtc aaagacttcc ttcaagcctt
    52921 cgtctaaaaa ccactgatag gagtccttct ggacctcgat cagattgggc atctctaaaa
    52981 cctcttcttg tcttgaataa ctcatacgca taaccttggt accggcaatc ggacgtattc
    53041 tgttcttttc cattgacatt tcaccccgtt ctttcagatt taaagcctat tttgcgtcgg
    53101 caacaatgca gtatatataa acgccagttt tttggcatga caaataagca tggtaccgca
    53161 caatagtgca ctatccataa tagcacaatg ttagaaatac gtcaaggaaa tattttgaat
    53221 tatttaaaaa gaatagatat ttttaaaaat taataactga cagatgcgaa taagaaaaac
    53281 catgcgccgg caggtgccgg cacatggtcc gtcatatact ggttaaaaat tacttcagag
    53341 taacctttgc gccaacttcc tcaagcttct tcttgatgtc ctcagcctct tccttagaag
    53401 cgccttcctt aacaacctta ggagctccgt caactacgtc cttagcttcc ttcaggccca
    53461 gaccggttac ttcacgaaca accttgataa ccttaacctt ctcagcgcca acctcggtca
    53521 gctctacgtc gaactcagtc ttctcttctg ctgcttcagc agggccagct gctgctacaa
    53581 caacgccagc tgctgcagat acgccgaact cctcctcgca agcctttacg agatcattta
    53641 actctaatac agataactct ttgatagcat cgattaattc ctgtgctgtt aatttagcca
    53701 ttttaatttc ctccatttga aatattttga ttgattgtca attagctgca attattattc
    53761 agctgtgggt gctgcttcgc aagctgctgc gccgcctttt tcagccagct gattcataac
    53821 acgtgcaaaa ttggtgatag gactctgtaa gctgccaagc agtttggaaa tgagtacgtc
    53881 tctggaaggg atgttagcga ttgcagccat acccttagca tcataaacag taccttctac
    53941 cacaccggcc ttgatctcaa gcttatcagc agtcttagcg aactgagcca gtactcttgc
    54001 aggagcggtt gcatcctcgg tagagtaagc cattgcgctg ggtccttcca gatactcgga
    54061 cagaccttcg aactgtgtac ccttgaacgc gaagttcatc atggtgttct tgtaaacctt
    54121 gtagctaact cctgcctcac gtaagctctt acgtaactgg gtatcctgct caacagtaag
    54181 tccacggtag tctaccagaa ctacagactg ggcatctttg ataccagcag aaatctcttc
    54241 tactacaggc tgttttaatt caacttttgc catttcttta cctccttata gattttgtgc
    54301 cgataagaag aatataaata gagtcctgct cgcaggattc tacgcctgcg gcgaatcgct
    54361 ttagcgatat attccacttc gccgcagtgc gatcctcgcg gagcgttttg ctttatagga
    54421 taaaatccta taaagtaaaa aaaactctct gtgaagacac agagagatga tatcatcata
    54481 cttatctcct cggcaggact tacgctcggt ctcccttgca cctgctgtct tcggcatggt
    54541 tttacaacct cagatagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    54601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca
    54661 aaaaccgcct cactgtaaac agtgaagcgg ctcttttgca ttcgttatct aattagatta
    54721 gagatcagat tagaactttg ctacgctaac cttaacgcca ggtcccatag tgctggtcag
    54781 agtgatgctt ctcagatact gaccctttaa tgcgctgggc ttagccttta cgattgcgtc
    54841 cataagtgca ttgaagttct cctgcagagc ttcctcagag aaagaagcct taccaacggg
    54901 aacatgcacg atgttggtct tatcaagtct gtattcaatc ttaccggctt tgatatcgtt
    54961 aactgccttg gttacatcca tggttacggt accagcctta gggttaggca ttaagccctt
    55021 aggtccgaga accttaccca gacgacctac aacccccatc atatcagggg ttgctactac
    55081 aacgtcgaag tccagccatc cttcgttctg gatcttcggg atcagctcct cgccaccaac
    55141 atagtcagcg cctgcagcct cagcctcatt gatcttatcg ccctttgcga ataccagaac
    55201 ttttacagtc ttaccagttc cgttaggcag tacaactgcg ccacggatct gctgctcagc
    55261 gtgacgtccg tcacaaccgg ttctgatgtg aacttcgatg gtctcatcga atttagcagt
    55321 tgcagttttc ttaaccagag cgatagcatc gttgggctca tagaatgtag cacgatctac
    55381 cagcttggca gcttcggtat atttcttacc atgtttcatt attttacctc ctaggttcca
    55441 gcggcaatat tttattcatt gcctcccaaa tatttataag ttactcctca acaacaatac
    55501 ccatgctgcg tgcagtacca gcgatcatgc tcatagctgc ttccaggctt gctgcattca
    55561 gatccggcat cttcatctca gcgatttcct gaaccttagc cttggaaatg gtagctacct
    55621 tggtcttgtt aggaacgccg gaaccggact taatgttgca ggctttcttt aacagtactg
    55681 ctgcaggggg agtctttgta acgaaactaa agcttctgtc tgcatataca gtaataacta
    55741 cggggatgat aagatctccc ttgtctgctg ttcttgcgtt gaactgcttg gtaaattcaa
    55801 cgatgttaac cccgtgctgt ccaagtgcag gaccaacagg gggagccggt gttgctttgc
    55861 cggcagggat ctgtaacttg atatatcctt ctactttctt tgccattgtg gcacctccta
    55921 taatgtggta attcggcgca ggctgccttg ttgcttgcct cctgctccca ctggaacgac
    55981 ttctcgttcc tttcatgctg ccactctctt caccttgcgg tttctgagag ttttatattt
    56041 tatctccgga attgcccgtg agaaaaaata tccttgtaag cttattaaag ctttctgacc
    56101 tctgcaaagc tgatttccac aggggtctct cttccaaaca tttctacatt gatcgtagct
    56161 gtctgtttgc catagtccag tttctgcacc acgccgacgg tatccttcca tactccggcc
    56221 acaacagcaa tgctgtcgcc ttcaccaaag tcgatggata cattctcggt cttaatgccc
    56281 aaaggcttca tctcagcttc tgtaagaggt acaggtttac ttccgggacc tacgaaaccg
    56341 gtcacaccac gggtattacg aacgacatac caggtatcat cgttcatctc catgttgaga
    56401 agcacataac cggggaacat tttcttggag accgtcttgc gggcgccgtc tttcagttct
    56461 acgacatcct gcatgggaac tcttacttca aggatctgtt ctgccagact cttgttgttc
    56521 tctatggtct tctcaatatt ggctttgacc ttgttctcat atcctgaata ggtatggact
    56581 acataccatt ttgcctctgc catacaatca ccctcgctct ataatgatgt taagaagttt
    56641 acgccgtact ggataaaata atccagaacc gcaataatca cacccactac aactgatatt
    56701 gcaacaaccg ctacggattg cttcaatgtt gattgtctgt ccggccatga aattttctta
    56761 aattcagctt ttgcgccttt aaagaaactg gggcgcttct gtttttctgc ggaatctccc
    56821 atttcgacct cccatactag aacggctgat tatttggttt ctttgtgcaa tgtatgtgtc
    56881 ttacagaatc tacaatactt cttggtttcc attctatcag gatgagtctt cttatccttg
    56941 gtagtattgt agttacgctg cttgcactcg gtacatgcta aagtaatttt tgtacgcatt
    57001 tttctacctc cacgtgtttt ctgatctcta aatttaagca taaaaaaaag acctaaatct
    57061 catctcgctt ggatactata acataatctg cggtcttcgt caactgtttt cccaaaaatc
    57121 ccactatttt tcttgtttct tcttatttat tatgctataa tgatcgcata aaagtggggt
    57181 attggatttc cccgtcccaa ttcatccgca gggaagaaag gagatgccta tgagtgctat
    57241 catgaacact gtctataata actacctgac cacctatgca tcaagacaga ttacaaaata
    57301 tgacacccat aaaaagagtg agcttcgcag tgtatacaac tctatcgtaa agctgaacaa
    57361 ggacactccc tggtatcttc ccaccaccaa taaagacaca cagcattatg ccgtggatct
    57421 gaaggaaaac gcccgcgaac tgcacaacac catcgcacag atcggcggac tggagacgga
    57481 tggactgttc cggaaaaaga gtgcctactc cacagacgat gatatcgtgg aggcttccta
    57541 cataggctcc gataccacca caggagcttc tccggaattt gatatcgaag taaaacagtt
    57601 ggccacgccg caggagaatc tgggttattt tcttccggat ctggcaacca cgcttgctcc
    57661 tgccacctat tcttttgata ttgcgatcaa cgatatgaac tatgaattcc agttcaatat
    57721 tggcgagggt gatacgaacc ggcagatcac ggaacgtctg tcccgtctga tcaacaacgc
    57781 ggatatcggt atcacagcag atgtgacgga atccgattcc cgttacgcac ttcgtctgac
    57841 ctcggatgct accggtgtac cgaacggcaa ggcctatcat tttcaggtca gcgatgatca
    57901 taccagcaag acctccggtg ttgtggatta cctcggactg aattatgtca gtcgtcctgc
    57961 cggcaacgca agattttttc tcaatggtga agaacgaacc gcttcttcca accactttac
    58021 aataggaaaa ctgttcgatg tacagttaaa agccgtcagt ccggaggatc agcccacaca
    58081 tgtcggtctg aaaacagata ccgaatccat cacagacaat atcatccagc tggtaggcag
    58141 ttataatgaa tttattaaaa cagcttcctc ctacctggag acacagtcca gaagcaaaca
    58201 gctgattcac gaattctcct ccatagcctc ccactacggt acttctctgg aaaacatggg
    58261 aatgcacctg caggaggatg gtattctatc tgtcaatgat gaaaagctgc ggcagaccgc
    58321 cgcggaatcc gggaatgacc tgtccggctt caatgtacta aaagaattca gcagttcgct
    58381 tttacacaag agtgatcagg ttgctctgaa tcctatgaat tatgtagaca aaaaaattgt
    58441 ggcctacaaa aatcccggtc acaattttgc cagtccttac attaccagcg cttacagcgg
    58501 tatgatgttt aacagctact gttgattccc ttccggggaa ttaacagtag ctgttatttc
    58561 attttctgct tctgttttct tattttgcct ttttggattt ggccttgtac ttatcaatct
    58621 gtacaaaatc atccatcagc tccagaatct tgtctctacc tgtctggttc agcttcacgt
    58681 agttctgcat aacacggttt tccagaagct gtgctcccag agaagcatct ctctccagag
    58741 aagagaaatc caccgggttc aggctcagct tgtcacacat cttgatcact gttccgtacg
    58801 ccatcccttc aatcccgcgc tccaatgcag tcaccagtgt gctgtaggga atgttcatct
    58861 ccatggcaaa ctgtctgaca ctacgatact gtctcaaaat ttctttcttt aaaatctccg
    58921 ccttagtcat atttattcct ccacgtgtcc tccaaaattg gtacagctat aatatacgat
    58981 ttcccgtttt ccatttcaag taaaaaacag aaaattaaga atttcgacga atttttaaca
    59041 atatttttat atttcttcca attgatccag ccagatcctg gcacaggcat cggacggcat
    59101 ccgcagatca cctctgggtg acaatgccac acttcccact ttcggtccgt ccggcaggca
    59161 ggaacgctta aactgctgtg tgaaaaatct ccggtaaaaa ttcttcagcc acttgcggat
    59221 cgtcggatcg tcatattttc cttcaaaggt ctctctcgcg atccggtaga ccttgtccgg
    59281 tgcataaccg caccgcagca tataatacag gaaaaaatca tgtagttcgt aaggacctac
    59341 cagatcttcc gtcttctgtg caatgttccc ctccaccggt ggaagcagtt ccgggctcac
    59401 cggcgtatcc agcacgtcct ttaggactgc ggtaagctct gcattattac aggtatcggc
    59461 ataataggcc accagatgtc tcaccagtgt tttgggcact ccggcattga cgccatacat
    59521 ggacatatga tctccgttat aggtagccca ccctaaggcc agttccgaca tatctcccgt
    59581 gccgatgacc agagcatttt ccttgttggc aatatccatg agcacctggg tacgctctct
    59641 ggcctgtccg ttctcatagg tgacatcatg gcactccata tcctgaccga tatccgtaaa
    59701 atgtctggtg accgattccc ggatatccac ctcccgcaat gtagttccca gcgtcttcgc
    59761 cagcagacag gcattctcat aagtccggtc tgttgttccg aaacagggca tggtcactgc
    59821 aatgatcccc gacctcggaa gtcccaacag atcaaaagtg cgcactgtca ctaaaagtgc
    59881 cagtgtggaa tccagtcctc ctgaaatacc caggaccgct gtcttacatc cgatatgctc
    59941 atatctcttt ttcaggccga aagcctgaat ggataaaata tcttcacatc tgcggttgcg
    60001 ttctaccatg tcctccggta caaacgggtg agcagcgaac ttccggtcca gttttgtccc
    60061 agtcagctgc atggaaaaag gaactgtaag atagggcttc atcggtctct ttccgaaggt
    60121 acccattcgc ctgcgctcac tgcttatttt taatacatcc agatctgtgt aaagaattcc
    60181 cgtagtaaaa cgagcggact gtaccaacag tgttccgttc tctgcgatca gatcatgtcc
    60241 tccgaatacc agatcctgcg tggattcccc ttctccggca gaggtataga tataaccgca
    60301 cagaagtctt gcactggaag cttttatcag aagggtacga taatcatcct tggaaaccgt
    60361 ctcattggag gccgacagat tcgcaatgac tgtagctccc atcagtgcat gcgaggtaga
    60421 aggcggctct gccacccaca gatcttcgca gagctcacat cccaatatca gctccggaag
    60481 ttcctcacac cggaacaaca ggttggatcc gaaaggaatc acctgttttc ccagacgata
    60541 ctccgtcacc gtttcctctc ccggtgtgaa atgtctgcct tcatagaact ccgcatacat
    60601 agggatattt ttcttaggaa tcattcccag tatcctaccc cggtacaata ccgcagccac
    60661 attgtacaga cggccgtcct tgtccacggg aagtcccacc agaaataacg tttccaactg
    60721 tccggtctct tctgcaatcg taagcaatgc ttcttttgct ttttccaaca aaagttcttg
    60781 caaaaaaaga tcgttacagg tgtatccggt aatacataat tccggaaaca ctacgatctt
    60841 tgctccatgc tccgccgctt cttttgcctg ttcacagatt acctgcgcat tatagaccgg
    60901 atccgccacc cggatcctgg gtgtggcgga tgctaccttg acaaatccct gtttcataag
    60961 cttcctcgct ttactgtata cattactgga tcatctgttt caattcatcc acggtaaaat
    61021 tatagttttt attacagaag tggcagttca cttcgatggt ctctccatca tcgatcatgg
    61081 actgcaattc ttccctgcca atgctgatca gtgcttttct cactctgtcc ttgctgcaat
    61141 tgcaggcaaa ttccacagga agtgtatcgg tgatctccag atccattccg gaaagcagta
    61201 cttccagcat tccctccgga gtcttgccgg catccagcat agtagtaacg gaagagagat
    61261 tcttgatatt ctcctccagt ttgctgatca tttcctcttc cgcaaagggc atcagctgta
    61321 caatgaaacc gcctgcctgt ctcacggtat tgtctctgtt catcaatacc ccaagtccta
    61381 ccacggaagg cacctgttcg gataccgcaa aataataggt cagatcctct gcaatctcgc
    61441 cggtctgcag ctctatctgt cccacatagg gatccttcag tcccatatcg cggatcacgc
    61501 ttaaaacacc tgcacctacg gcaccgccca catccagttt tccctgggca ttggctgaca
    61561 ggatcacatc gggcacattg gcatagccct ttacatgacc gttcatatct gctgtaactg
    61621 tcaggccccc aatgggacca tctcctttga tctgcagtgt gatcagttca tcgtcaccct
    61681 ttaacatgct tcccatcatg gcgcccgcgc tgagtaatct tcccagtgcc gccgttacca
    61741 cagggctggt attgtgagac gctcttgcgg tctccaccgt ctctcttgtc gtgcaggcga
    61801 acgctcggat ctgcgcattt gccgcggttg cacgtaccat ataatctttc atttattcca
    61861 cgtacctttc tgtctacagc tctttctgct gctcttttgc tacgatatat actctttcac
    61921 tggtctccgt taccgcctca ccggtatcgg aatccatgat ctccataatc ttcagtccgg
    61981 cctgttccac cagtgtcctc atctgttcta cggtataacc cctctgcaaa tgggtctcgg
    62041 taaatctgtg gaaggtatca ccctcttcct ggacgaagat tgtcagatca tactcgttga
    62101 tctcttcttc cggatcataa taattttccc agataaaact gcaatcctcc cggttctccg
    62161 caatcgtcgt attgccgatg acttcccggt atttataatc tgtattaaaa tcaaagataa
    62221 aaatgccacc ggggtagaga taattgttca ccagtcggaa tacctgcaaa agttcctctt
    62281 cttccaaaat ataattgacg gaatcacaga cgctgatcac cgtaccaaca gtagaataca
    62341 gatccagttc ccgcatatcc tgatgcagat agaggatctc cgaaccgctc tccacctttt
    62401 tctccatggc aatgcccagc atctcttcag aattgtccac gccgatgcaa tcataccctt
    62461 tttggtataa cagttctgtc aaagtgccgg tgccgcagcc cagatccacc accagattcc
    62521 gctcggaatc cagaattcct tccgcgtccc gcgtaggtct ggatacacca taggacgcaa
    62581 tcagctgatc cagacgattg caccacattt catagggtgt attatccatc agttcgtcat
    62641 atactcttgc aaagtctgta tagatttctt ccgtattctc cataattgca gcatctccag
    62701 ttttttagaa aatagtcccc agtgcgccga atactcccat gctgagcaat cccatgatca
    62761 cgcctgccag aaccacaccg cacattactg caatgacact ggacttgaaa tccatgtcta
    62821 acaaacttgc tgccagtgtt ccggtccacg caccggttcc gggaagggga atacccacga
    62881 acaacaacag cgccacgaac aggcctctgc ccgctttttc ctgcagcttt tgtccgcctt
    62941 tatgtccttt ttccaggcag aaggtaaaga acttgccgat gaccggcttg tccgcacccc
    63001 attccagtac ttttcttgca aaaaagaaga taataggtac cggcagcata tttcccagaa
    63061 tacagataat atagctggga accaaaggaa gattcattcc aaccgcaata gggacagcac
    63121 ctctcagctc gatcagagga accatagata ccaggaaaat catcaaatat ttttttaaca
    63181 tgtgtttact ccaattcttt taatttattt atgcccgctt ttccagataa tttttcaaaa
    63241 aagcgatcaa tcggtccatt tcctcatccg tgccgactgt aatacgcagg gagttggcaa
    63301 ttctcggttt attccagtgt ctgacataga tgtcagcctc acggagagcc cggaacagtt
    63361 cttccgcggg aacttcctta tgagaagcaa atataaagtt tgctttcgaa tccgggaaag
    63421 taaagcccag ctcgttcagt tccttcttga tccgctctct ggtagctgcg atctttgccg
    63481 tggtggcctt gaaataagca tcatctctta ccgcttccac gcccatgacc tgggaaggca
    63541 ggttcatggt ataggagttg aaggaaaact tcacatcgtt cagatagtgt atcagcttct
    63601 cattaccgat acagaagccg atacgcattc ccgccatggc tctggatttg gaaaaagtct
    63661 gtactaccag aagattctca tattttttaa gcaagggcag tgcacttgtg cctccaaagt
    63721 ccacataagc ctcatccacg atcactacca cgtccggatt ggcctgtaag atttcttcga
    63781 tcattgcctg ggactcttcc acaccggtag gtgcattggg attcgggaag atcacaccgc
    63841 cgttgggctg tttgtaatct tcagggttca tgtgccagtt ctcatccagc gcacaggtat
    63901 gataaggaat ccggtacaga tccgcccata catcatagaa ggaataagtc acatccggga
    63961 acaggatcgg ctttccgctg ttgaaaaatg tcagaaatgc catggataat acatcatcgg
    64021 atcccacacc tacaaatacc tgttccggtt tcacaccgta atatgctgcc aactcgtcca
    64081 ccaggggctg cgcatttgaa ttcggatata aacgcagtgc cgcactctcc atattttctg
    64141 ctgccttccg cactcccggg gcaggaggat aaggacactc attcgtattc agcttgatca
    64201 tgttcgggcg gttcggctgc tctcctgcca cataaggaac caccttacgt acgttttctt
    64261 cccacttcat gttaataacc atgcctttct caaaacctgc aagctttcag agactttcgt
    64321 cccttgtgca ggcacaaatt tgcgtgtgaa aaatttattt tcagttatct gtttgttcat
    64381 tctgtcgatg ttttaattct tccgcatagc tcctcgcctg tgcaggaagt cccagatgtt
    64441 cgtaacaccg gatcagcccc tgtaaaggtt cttcttccga actccgcagc gccagtaccg
    64501 gctgtgtttc ataagctgca ttataatacc agacagccgc ttcctcaaag tctcccgtct
    64561 cttcatagaa ataccccagt tcacagcaga tttccgaaca gccgtccccc gcgatcacct
    64621 tggaagtata cttgaaaaat gtcacagcag ctccccgcag tctggcggct ctggccacca
    64681 cacaacagcc ttccgtaatc tcatccgcac ttctgtccgg ggacgaaact tctgccctga
    64741 aataatcttc cgcttccaga aagtcccgtg cctctcccgc caggaacagt tctctggcat
    64801 acattccgta cagtcttctg gaaagtcgtc ttccctgcgc gatctgtttg cggaaatttg
    64861 tcagatccct gcccgcatga ttctgttccg gcatgtgagt gatgactata tcgctgtcat
    64921 agactaccgg ttccagccgt acagtttcat ggattggttc ttcccacacg aagcttcgca
    64981 gtctgcggaa taatttggga cgatattctt catcaaaatt atacactgtg ccgttctgta
    65041 gctgattgcc gtatttcatc tggacgatct cgatctccgg aagcagtgtc tcctttaaca
    65101 gtcgaaacct tcgccggttt tcttccgaaa gcacctcatc cgcatctgcg gaataaatgt
    65161 attccttcgt agctttggaa aatgcaaaat ttctcgccgc actgaagtca tcgttccatg
    65221 tgaaatcata tattttatcg gtgtatccgg ctgcaatccg ctttgtggca tccgtggatc
    65281 ctgtatccac aataatgatc tcatccacca gatccctgac actgtccaga caccttttca
    65341 gaacccgttc ttcatttttt acaatcatgc acaaagatat tgtgatcatt ccggatctcc
    65401 gttcccgcat ttttgcatgc gatgctgttc ctacagcact ttggataaga attcctgtaa
    65461 tcttgggctc ttaggattgg tgaagaactc ctggggagta ttctcctcca cgatattacc
    65521 accgtccatg aaaagcacct tcgtacccac ttctctggca aatcccatct catgtgtcac
    65581 gaccaccatg gtcataccgc tctctgccag ttctttcata acttcaagta cctctcccac
    65641 catttcggga tccagtgcgg aagtaggctc atcaaacagc attactctgg gattcattgc
    65701 cagagatctg acaattgcaa ttctctgctt ctgaccgccg cttaaagatg ccggataagc
    65761 atctgccttt tcctccagtc caactctctt taacagcttc agggcattct cttttgcttc
    65821 ttcctcaccc atcagtttca atttcacggg ggcaagggta atattgttca tgatcgtcag
    65881 attgttgaac aggttaaaat gctgaaatac catacccata tgctgacgaa ctttgttgat
    65941 gtcgatcctg ggatccgtga tgttctgtcc atcgaaccag atctcaccgc tggtaggtct
    66001 ctctaaaaga ttcaggcagc gtaaaaatgt actcttaccg gagccggaag gtcctaccac
    66061 cacgatcttc tcaccggggt gaatatgata atttacccct tttaatacct gattatcttc
    66121 aaagctttta tgcagatctt taacgattat cactcttacg cagtctcctt tccagttttg
    66181 caagcagaat ggacaatacc ttggtcagta caaaatagat gaaggcaata ccaagcagcg
    66241 gtgtaaatac atcaaacagc ttggaggtca tctggttggc cacacgggta aggtcactca
    66301 ttcctacgta acctacaata gaggtttcct taatcagcac gataaattca ttgcccagag
    66361 gcggcaggat gctcttgaat gcctgcggaa tgataatgta tcgcatcgtc tggaatttgt
    66421 tcagtcccag tgatctgccc gcttccattt gtcccttgtc cacagccagg atgcctgctc
    66481 tgacgatctc cgccacataa gcaccggaat tgataccgaa tgtcagactg gcgatcagca
    66541 cactgttctt acaggtagcc agaatgccgg accacatgat caacaactgt accgtagtag
    66601 gagttccgcg gataatatca atataagcac ttgcaatccg ggacgctatg gtcttcgatc
    66661 cgttttttct cgtggataat ttcaaaaatg ccgctatggt tccgatcagg ataccaagca
    66721 gtgccgcaaa aaaagaaacc tctaaggtta ctcccagtcc tttcagataa agcttccatc
    66781 tgtccgcttc aatgaaggct gcataaatat gttgccagat attttgcatg ttatttccta
    66841 tgcctttctt gtgaaaatat aagtgtacga ttggtcaaac cgtatatgtt gcctacctta
    66901 atatagcaaa aagaagagaa tggcacaagc cattctcttc tttttcatgt atattttgta
    66961 taaaaattat tcaccgatgt actcagctac cagtgcatca aaagtaccgt cagccttcag
    67021 atcagccagt gcctgattta cctgctctac caactcgctt cccttaggca gtgcgatagc
    67081 gtattcctca gcttcgaagc cgaaatcagc accgtcgatg atcttaacct taccctcgaa
    67141 tttggtatta aatacttctg cagggttctt atcaatgata acagcgtcta ctctgccgtt
    67201 taccaaatcg tttactgcat cgattgctct gttgtaggtc tgtgcggtag caccttcgat
    67261 atcctctgcc aggaacatac cggtggtacc cagctgacag ccgatggtct tgttcttcag
    67321 atcatctgca gctgcgatct cggaatctgc cggtacgatc acaaactgta cagcttcata
    67381 atacgcatcg gagaaatcta cctgctcctt acgatcatca gtaacggtca ttcctgcgat
    67441 agcaccgtcg attctgttgc caatggaact aaccagagag tcaaactcca tattctctac
    67501 ctgtacttcc atacccagtt tctcgcctac agccttgatc agagctacat cgaacccgtc
    67561 gggttctccg tcatctccta catactcaaa gggagggaat tctgcgttgg taccgaatac
    67621 cagtacgcca ccctcggtct tatcagcggc ctcagtagta gctgctactt ctgtagtggt
    67681 tgctgcagca gtctctgcca cactgctccc ggaagcctga gagctctcgt ttgctgcgga
    67741 atcagaacca cagccggtca acattgctgt acacattaca gttgcaagta acattgcgat
    67801 tgcttttttc ataatattat cctcctgtct gttttctacg gacgacagcc gtccttaaac
    67861 gttgattact atagcacctt tttgaaaatt gtgcaactgt ttatgcataa atatacgcag
    67921 ctttactctg caaatacaac attaccggga tctgcaccca gaaggttacc actatatatg
    67981 gaataaacgg cttcggtaca cgaagttttc catccggtgt cacatttctt ctgcaaaact
    68041 gtactgcagc cagcaatgtc aacgccgata acatatggat cagaaaccac aacagattcc
    68101 cctgccaggc gcagcctgcc aacgcacata cagaaaatgc atgacagtca gctcttccgt
    68161 acatttgggc aaacaatatc tgctgcagga gtacaaacaa tgccactgct gccgcctgct
    68221 gtatgctaaa cctgtcagta atcctgcaca ctccatcctc catataagct tccgacaccg
    68281 gaagccccag cagtattcct gtccatggca ggcaccacca ccatacataa ttatacacat
    68341 aactgtgtac agcatccatg caggccgccg ctaacaaacc tcccgccagc agggagcaca
    68401 ggatctcctg tgcccccagg atcaccagtc tgttttcttc cggaagtctt ccatccattc
    68461 cccctataac aagaatcaca actgcattga tccaggccag tcccccgaga agccacgctt
    68521 ttccgggttc catcctctcg atgtctcccg atagttccac acgcagcagc cagattgcac
    68581 taccatagat aaggcctccc agaaccaccg accacagtat ccggaacacg atttccaagg
    68641 ctctcatacc tccttaccgt aaccgggtac gcagttcatc caacacattt cccgacactc
    68701 cgatcacgct gatcgtcgga tgatcttcca gcctcttgtc acctttaaag gcccgcactg
    68761 tgatttcctg tgtctggttc tccggagtat ataaaggcac cataaattcc agttcttccg
    68821 tcttacgata cccctgactt tcggtataat catagacttt attcaatgtc ggatccagtg
    68881 ctgtcataca ttccgggaaa atgacttcca cccggtccgc atatccccat gtggtaaagg
    68941 taagaattcc gctttcgccg catttgaaga ccggctcctg cggctccaaa atacgtttca
    69001 cactgctttc cagtgcaaat tccgtcgtac cgtaacagat acggctctca ttccccacat
    69061 gatccgaagc atatcccagt accgtaaaat ccccgctgaa cagcggttcg tcctctgtga
    69121 ttgtaatcac gatacagcca tcttctccgg gaaggtaggt cttagaaaca gcattgtccg
    69181 tattactgat aacaattgta aaatgatcca caccacttag agcatctgtg gcagtaacac
    69241 gtatggtaac agctccgtct ctgcgatcga tcagtgcccg gttctccagt atttccatcc
    69301 cccggatgac cggagcttct ccgtcagcga tcagagcgat cccgtttttc tgatcctgcg
    69361 tagatacaga atatatttta tctgttccgt gatccgcttc cgctaccgga atcacccgaa
    69421 tcgtttgtcc gttccggtct ccacttatga caaattgctg tactccggta agttcctggt
    69481 tccggtgtga ccgcaaagta taggaatagg aatgttgctg cagcacattg gtcccctcag
    69541 tcgcataaga cagtccctcc gcatctgtcc ggatcacatc atccgttatc tccgcagacg
    69601 gtgtgacaat gatatttctc gccctgccac cgtcctccgt caccgtttca tatattgtcc
    69661 cattcagttg atagtccggt ctggctgtac cttccatata agatgcattc gttaacgtga
    69721 acggtgtctt tccgtccgcc cggacatacc aggttttgtc cgctgccgcc ggatatacat
    69781 tgccctctgc ctcgtttatc ttaagctgtt ttgtatgaag cttccacaga atctgttttc
    69841 catccacaca aatatgtgtg gtctcgctta catttcccgc cacatccaca gcggcaacat
    69901 gcagatactg caccccatcc tttacctcca gttccatatg gggttcttta caataaccgg
    69961 catcgtccgt tacgacggta tccctgtttt gatccacaag ataataatat cccctgatac
    70021 cgctaagcaa tgtgtttttt gtgatattgg agtcacatag tttggaagta ctgcccgtca
    70081 gataagattc cgctctatga taatataccg tcccgtaatc ctccggctct gtccacgaga
    70141 ttcttattct ttcactgtcc agtgcttctc ttacgacgtt ctccggaatt ttcccgggag
    70201 ccgcaagatc agttgccctg actcccttca gctgtaaagt ctcctggtaa cctattgctt
    70261 ttgactgcag acacaacagc ccgtctccac tccgtactcc actttcactt tcatatagca
    70321 gtgcacattc cggatttaca taattgcttc cgccatatgc cggcttcgag aataggacct
    70381 ggccttccgt ataaccgcat acaagagttc tcccaccact gaaactaagt gagctgattc
    70441 ccgcggtaaa ccagacattt cctcttaaat gaaaattcag atgcacgtag atatgtgtcg
    70501 ttccttccgg taatgagata tgcagggact gccggaaatc actggctgcc gggtctccgc
    70561 cgccatgttt atcataaaaa ggataatccc gaaattttcc ctgtgcaatt tttgttcctt
    70621 tctgatccag gattacaaat tccgacggct gcttctcatc cggcagccag tcggtattca
    70681 caatatttcc acccagagat ccccatccat cgcaaaaata tgaaagattc aatgtcgtat
    70741 tgccattggt gggaatcccc cgataaccgg aaacattcca catctgttcc ttaggataat
    70801 tcccgtttct ggcccatccg acccggacta tggcacggct ccactcatca tcgtccgtac
    70861 ttgtggtatg tccgttatac tgataagaat ttgtgcctga ggaatcaaag caattcgatc
    70921 cccacataaa atattcccaa tctccgaatg ttggtgtata agaagcattt ctatagcagt
    70981 cattcgtatg gtgatgcacg atcttctctc cggcggttcc tccataatat ccggctccgc
    71041 ctccggccat acccgcttct ccttcctgct gcccgaggac actttccatg ctgccaccct
    71101 tgccgcctgc accgcccggt gatgctccac cgcctcctcc tgcgatcagc aggattccct
    71161 tccggtcgga taccacggat gtcattcctc ctccgtttcc ataatttgaa gctctgccgc
    71221 ctttgttata cccgttttga ccacccacgg cataggtcag ctgttccccc ctctgcagcc
    71281 agaatgttcc ggaggcactt cccccacatc ctccccgaaa ggaaccgtag cactgtccct
    71341 gcgcccccat cgcctttatg acgtagagtc cggtatacgg cacgttatat gtcctcgtct
    71401 ttgcttcata aataccggtc agttcaacgg ccgctccgct gttgatatcc gttgcactat
    71461 taatccgcat ccaggcaccg tcttccagtc tctgataaat aaggtacgtt ttgttgttgt
    71521 gatccgtctg ctcccaggac agatccaccg ctccttttcc ttcgttttcc ttgtagttat
    71581 ctttcgccag cagggtaaga tccacccatt gcgcgtacaa tgtcgtattt tcggacggta
    71641 tgatgctgtc gcccgctcct cctgccggcc gtcggaattc cgagtcataa taccagcctc
    71701 caaaggacca cccgggtctg gtggtctccg gtaatgtgac aggttccggc tgatagactg
    71761 ccgtcagcct gtccacatta ccgtccggag catcaaagat atattgcttt ccatccagac
    71821 tccccctgaa cggatgcacc tttttccatt cgacaaaccg atttttccct tgcaccggag
    71881 tgatctccga tccgccattg gcacgaaaac agactgtata cccatctggt gcgcagacca
    71941 gttcttcctg caacagatat ctttcaagat atggctgcgt tacagaaaat atctcccggt
    72001 catcatactt tcctccattg gcataaagca caagggtgct ttcactccgc acccattgtg
    72061 catataaatt gactacacca tgatcgttat ctgtccaggt tgtccggtct ctccaatccg
    72121 cctgcctgag attttcgatt gcagctttat ccgcataagc ggttcctgaa ccatctggct
    72181 ctgtgttcca gcccgcgaac aaatatccgg tcctgctgta attgttctct gtcaggtgtg
    72241 tcaccggagt gaccgtcttg ccttcgtaga ctatggcatc gttgtacata tggatactgt
    72301 catccatata accgttataa gatgcacctc ccgtattggc ctgataacag accttatact
    72361 gattccagga aatattttta catttatgtg ccggcagatc caccccctgc tccagattgt
    72421 cgcagaacgg acagagataa tagtagctca tcggttttct ttcgttgccc agatccaggt
    72481 actgtatcga ggccgccgct tctctgctca tatagatatt gtactcttca atggcttctc
    72541 cgcaatccgc acaataggct ttccagccgg aataataata ctgcatgcat ttttgtctgc
    72601 caaaagatac cccgtgccag ttttttccgg tatatccatt atgaatacac tgtcccggcc
    72661 ggtggaccac tttccaatat ctcactggga tttctccata ccgtttctct gtataaatat
    72721 gttctcccac taccggatct ctcagcgaag aaggaatctg cgtatacaca gtggtgctgt
    72781 catcccgggc ataccacaca taattccggt ctcctgcgca ggttgtaaat gcacgtccat
    72841 ccggactgaa agaaacataa ggactgttgt aaatggtctt tttcgctgcc gcttccgact
    72901 gtatcgaaaa caggagcgca attcccagca cgagtaatcc tgccagatat ctcccatacc
    72961 gcattctcat tcctcctgcc tatccgtatc ctctgccttg accacacgca aggagccctg
    73021 tgccagtgca gcataaaagt ccgccaccct ttctttttcc atatagatat ttactctctg
    73081 tgctggagtt gtcccatcat caccgccgat tgcagctcca ttccggtcaa acacctgttg
    73141 gacaaacaca ttttcccaca acagtttccc gttttgttcc tcttcctcct gttccacaca
    73201 ataaatatgg atcctgtctc cggcacgcag gacacctccc actacctggt acagatcttc
    73261 ggcgcggaat ccggctataa caggttcccg catctgctct gtgatctccg acagtgcccg
    73321 cagcatgcct gtagtcagta ccatcccctg ttccactcca tatgccgcta ttcttccgct
    73381 tgccgcctct gtgcttttca gtgcagtctc cggcacaatt cttttgtcga tttccatgac
    73441 tgtaatatac tgctctgcat tttccgcgga gattttttct cccctgggaa ttgctgccgt
    73501 taccacgcat acctgttctt tttcgtattc cgtaagcact ctcttttctg tctgcagcaa
    73561 aaaaacaaat actgccatag ccgccacaaa cgctgccagg atacttccgt tccagattct
    73621 attcttccac gcctgactca tcttccgttt tttcattcgt ttcctctccc atatattcac
    73681 tgaccacatc taccagctta cttttttcag cagttactgt aattcccaga aaaccttcca
    73741 ccggatatgt gatctcactg tacacaccgg tattctcaat ggtctgtccc cccggagtcc
    73801 tcacttctcc cagggttcct tcccattcca ggatccttcc gtcaccgttt cgttcccaca
    73861 cctgcacttt tgttcccgtg acattataga taatgtaatt tcgtatctct acctttccgg
    73921 aaatcagctt gtgacctact gcttcataat tttcatttaa ccccagattt tcacggacag
    73981 cagaacaata tcccgcatac gcctgttcct gatctgtgat atgtacctta tgtgtgctgc
    74041 catactcccg gatgtcaatg actgccgatg ccagattgga cagtgccaat gcatcttcca
    74101 gataccgtgc agatgccttg tacattgcca gttgcagctg catatacaac aaaattgcca
    74161 gaaacagtat cagaaacaat cccagcaccc aggaaacctg tccgctttct gcttttcctg
    74221 tctcctgccg gttctcagtt tttggcagtc gatactctct tttcccgtat ttcataatgt
    74281 ccgcccagct ttccgtgaat ctgcaggata atgcgttccc catatcctgc aggttccaaa
    74341 gtacttccct cataatcgat ctctgtcagt cctgccgtct ccagttctgc cgtgaggtgt
    74401 gcctgctccg ccggttccag atatcctacc gtttccattt tcagcagata ggccctcgcc
    74461 agctgtccta cattctcttt tgcctggagc agctgcacat tctgcaggta gttcagcatc
    74521 actaccgtca gtgccagcac acacatgccc atcgtcagca gctctcctat gttccctggc
    74581 tgtttccctc gttccgtcat cttctctcct atcctatacc cctgtcagga tatctgccgc
    74641 tttggtctgc atcgctgtta caatggtctg tataaacgtt gtcagactgt ccttcatgac
    74701 cacgcacaat aacagtgcaa taatacacag acctacagtg accaggatcc cgtcgatccc
    74761 ctgctcctgc cgtaaggcat gttcttttac ttttccccag attctctgta aattcttcat
    74821 gttttgtccc tttcttgttt tgttttattg gtatttcaga attgcaccac tgcccggaac
    74881 atatatccca cacaggcata taaaatgatt cccagtaccg cccccagcat tcccagctga
    74941 taaaaagtaa tactacggtc cagagcactt ttttgctgtt gaagcaccgt aagttccata
    75001 tccttcaact gctctcccat gtccttcaac agatctaccg cctgccccga ctctcctgcc
    75061 tggatcaggg taatgcacaa tgcatcgatc tgacggttgt caaatttctg ttggaagtca
    75121 gacaaagccg taaccagatc cgactgtatg accagatctc cggacagttc catcaaagcc
    75181 tgctgtaatc tctgcgtccg tacgctctgg cagcattccg tcaaggcatc cgttacatag
    75241 actccggcac aggtctgtac ctccagtgcc tgatagatca gctgcagatc cggcatcatc
    75301 ttacgattgt cctgtcggtt caggtacagc aataacagtt ccggcacata atacagtccc
    75361 attgccgcaa gcagtgcata tccccagccg atagattcca gtgtcagtac tgcgccaaga
    75421 gaaagaaaca ggcgcatcag caggtatttt accggtgtga ttttcttccc atagtgaaat
    75481 tttgcaccgt ttttccttaa tttcttttct gttccggtga cccagttttc ataccttcgg
    75541 ctctcccgca gcattccggt aaattcctcg caggcatgca acaccgtttt cggcattgtt
    75601 cccataccca gccggaacac aaaaattcct cccagcaccg ctcccaacag ttcgggaatc
    75661 cagataactc tccagctcat tctccacacc tccctgacta gcggcgaaaa ccatacagtt
    75721 tccgcaaaaa caaaaacaat atcagtccat agacacccac tgcaagatgc cccggtaaag
    75781 tcccggatac tattttccag accgacacat cgatcagccg atccaccgtc agcagggcga
    75841 ataccgacat tcccagaagc actgccatat tgatacctgc ctctcgaagc atccctttac
    75901 gttcctcttc catacgcaga tactcccttg cggaacgtct gctgctgtct accagtgtgg
    75961 tcagatctgc catgtagcgg atgctgatct ccagatttct ggccagttcc tttatcttgg
    76021 gatgttctat tcgttccgcc atggacaaaa gtgccagcga actgtctccg gtcgtctgtg
    76081 cttcataggc gcattcctcc agtgctcctt ttaacggctc ctccacataa cggctcacct
    76141 gacccagaac catggtgatc tctcccgctg tcagactgta attccccaga aaattcagac
    76201 atttcagcag attttcattg gtcacccgca attcttctgc ccgcagtgtg ctcaggaaca
    76261 gatattccat ggctccgcat atcattattc ccaataaagc tacctggaat ttcccgctgc
    76321 ttccaagcaa cactccaaag atcaccgtca ttcccagaag atttcctacg atccatttct
    76381 ccggggtgca cgccggaaat ctgcgccgca taccgcaata acagagaaat tcttccaccc
    76441 ggcagatcca ggtattgtta cgctgtagtt cctgcagctt gtgccggttt tccaataccc
    76501 gcagtcttgc cgactgatcc atcccttttt tcgtccggga cagcaggcgt tttgccgtct
    76561 ctacccaccg tatccttcca aacagaagca gaaaacccgt aaatattaaa caaaagatcc
    76621 atacgtatat gatcataagt ctctcctctc atgtaatggt caaaacaatc tctcatactc
    76681 cagattctct ttctccgcat tccaccccag acaggcgaag atctccttca cctggtaatg
    76741 ttccataaac accagggtct gaaagcagtc catcattttc attaattcgt tgcgggaata
    76801 acgactgtca tacattgcat agtccacgat cttctccggt gccctgtccg cagagggtgc
    76861 atgtaccgta gccgcacaga tctgtcctgt atacgctgca ttcaacagat acaatgcttc
    76921 tcctcccttc acctcaccta tgataaaaaa atccacgtcc atcgtcagtc ccgctatgct
    76981 gatatgtttt aaatcataat tcacactgct ttccgaggtt cccggaagcg aatgaaggaa
    77041 catcatatcc ggatgaaaca gtgtggtcag ctcatccgcc tgctgtgcca ccagaattgc
    77101 catatcatcc ggaagcgtct ccttcaacgc atttaacagt gtggttttac cggaagaatt
    77161 tcctccgcag atcagggtcg atccctgtcg gaacctctgc accagcagtt ctgccgtatt
    77221 cctgtccagc atctctttct ccaccagttc tgtcatcatg ggaaaactct tcggcacctt
    77281 ccggatacac agataaggtt cgctgtaggt attgacaatg ggcatggata gggtaaaacg
    77341 aaagataaag tccggatgac tttctgtatc cgtaaaccgt tggatcgcat tcaggttaga
    77401 aatattcacc tgatttctgg ttgccacata atcaataaac tgccggtact ccttctccga
    77461 agcaaaggca attccggcat ccatacgttt tccttctctc ttgatccgga tacagtcatg
    77521 actcaccacc cggatatccg aaatgctttt gtcatccatg agcggtgaca gtctggaata
    77581 accgaaaata tattgttcaa atgattttaa aagtcttttc cttttcgttg ccgggaaatg
    77641 ataatattga tcgatatgtt ttcctgcctc ctgcagaaat tcctccttgc ccaacacacc
    77701 cttttttacc tggatcaaaa gcacctgttt ctgtgtgata aaatcttcta ccagcctctg
    77761 caattctgca tcagaggcgg tttcttccct tttgttttct ctttcctcca tacatcctcc
    77821 tgttggtttt tctcaaaata atccattttg cactaattaa tacattatgt gattcctggt
    77881 cgaatatgac ctgatattta gaattttgac ctcgactgac ctgtcgggta atatgcagat
    77941 tactcccttc cgtggcatct gtcaacgcct ccggaactat tttctcattt tcggaagaat
    78001 ggccaaagaa aaaccgcatt ctgtcaggtt aatttcaaaa agcccgtcag aatgcggtct
    78061 gcgcatttgt ctattttgtc tttgtctatt ttgccatatc cagctcccgt tcctgctgcc
    78121 aaagcagttt gtcgaactcc tccaccggca tcggccggta gaataaaaag ccctgtacat
    78181 cggtacaatt acagcctaac aggaactcct tctgttcttc cgtctccaca ccttcaatga
    78241 cgatatgtgc tcccagctgc tgcagcatgg caatggtatt gcggataatg atctgactct
    78301 tatctttctc cagatcccgg atgaattccc gatcgatctt aatctcatcc aatgtctgat
    78361 tctttaacat gttcatagta gaatatccgg taccgaaatc atccatggac accaggaacc
    78421 ctcgctcccg caggctttcc atgcggcgat acataccaac ctcgtcctcc aggaatgcgc
    78481 tctccgtcag ttccagcgga acatactccg gcagaacatc atattcctcc ctgagacgga
    78541 gcagtgtctc tgtcagtttc tcatcatacg catgcaatcg ggatacattg atggaaacgg
    78601 gaataagtgt ccggccttcc ttacgcagct ttccaagata agcaaacact ttctcccaga
    78661 tatattcatc tacattgatg ataaatccgt ttttctccag tacgggaata aactctcccg
    78721 gtggcaccag ccccttttcc ggatgcttcc agcgcacaag cgcctcacct ccgatcactc
    78781 ttccggccct catatcgacc ttgggctgca catacagctg tagttctccg ttctccagtg
    78841 cggacacgat atcattttcc acctggcgtt ctcgcatctt ctgactacgc attttttcat
    78901 caaatactgc atatgtccga tatactttgc ctttaatact gttcatagcc atcgtggcgc
    78961 aatctttcag atagctgatg gcaggggctc tctccggtga gattccgatt ccaaaggatg
    79021 gttgtacacg gtaagcgaac ggaaagtcag ttatcttttt tctgatctcc cgcacaataa
    79081 tttccagttc ctctacttcc tcatagcttg tgcataagca aaagatatct gctctgatat
    79141 ggcaggcata gctatccggc ctgttattct cataccagtc aaagcaagac gcaataaatc
    79201 ttagcaatct gtctccttcg tctctgccgc agaactcatt cacagcctta aactgtacaa
    79261 tatccataat tatgacaccg tattttttat ccggctgctg cttgatcatt tcaccgcaca
    79321 gatagaaaaa agaattgata ttatgcagat ttgtcagccc tgttctgtta ttttctacat
    79381 tttcttccgc catttttcta agagaataca gatataattc ttcttttccg gcatctgttt
    79441 tctgcggcga agattcaatg acttcctctt ttgctttctg atacgtcata ggcaccctcc
    79501 tcaaaacata aaccagtccc gatgaatgct tttattatac ttcattttgg tgattttttc
    79561 tactatatgc cgatttttat tgtttttttt aggaaaaatg atataatatg ggtgtctgta
    79621 aagttatttc agagaggagc gtactttatg tttttacttt tagccggcgg acttttgatc
    79681 atccttttgg cagtcgtgat tgctgtcgta tcttctgtag tttccgctgt tgcagcagct
    79741 accgacgatg cagaagactg acattccgga cttcggactg tcagttttct tctccccgtt
    79801 cgattctcgc cgccaggtct tctaagtaca tccagcgttc cattttttct tccagctgct
    79861 gttccacagc ctctttttcc tgtgttaatt cattgagttt cacaaaatca ctggcatatt
    79921 ttgaaaaatc tgcttccaaa gttgcaattt tatcctctaa tgctgccatt tcgccttcaa
    79981 tggattcata gtctttctgt tcctggtaag tgaattttaa tttgcgtttc tgtccgtttt
    80041 tccagttttt agaagtagtt tcctcatcgg cgttcttcgg ctgggatgct gccgccgttc
    80101 ctgtgctctc tgtgttcccc tccgcagctt tcctattggc ataatctgtg taaccgccct
    80161 catactgcac catgcgggta tcctccaggg cgaagatcct gcg
//
LOCUS       DAWEJW010000003        59380 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_265_length_59380_cov_53.823329, whole genome shotgun sequence.
ACCESSION   DAWEJW010000003 DAWEJW010000000
VERSION     DAWEJW010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 59380)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 59380)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..59380
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_265_length_59380_cov_53.823329"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    28764..28863
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    30516..30615
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        16624 a        12294 c        16290 g        13972 t
ORIGIN      
        1 ctatatgaac aaagaagtat tatttgccaa gaccttagag caggtccgca agaccgccaa
       61 ggagcagggc aactgcatca gcgaagaaca ggtaaaggaa gcattcgccg agctggatct
      121 gagcaatgaa cagctgcaga tggtatttga ctatctgtta aaacataaaa tcggtatcgg
      181 acagcctatg gatcccgatg aattcctgac ggaggaagaa aaggattact tacaggaata
      241 tctggatgaa gtagcggcat taccgaccta cagtgacggt gaaaaactgg cattttccat
      301 tgcggccatg gcgggagaga cggatgcgca gcagcgcctg atcgagattc atctggcgga
      361 tgtggcagag atcgcaaaac tctatgccgg acagggtgtc ctgctggaag atctggtggg
      421 agaaggcaat ctggcgcttt ccttcggtgt gaccatgctg ggcagtctgg agaagccgga
      481 cgaagtcgag ggaatgctcg gcaaaatgat catggatgcc atggaagaat acatagccga
      541 acatgcagaa aattccaaga tcgacaagcg agtggaagat aaggtcaaca aggtagcgga
      601 taaggccaga gaactggcgg aagaactgca ccgcaaggtg accacggagg aactgatgga
      661 agagaccggt atgtcccgca agatgatcga ggatgccata agaatgagcg gtttcaagat
      721 tgaggatatc gacaacaatg caaaagacag tttatgagaa ttataaatac agcctgcagg
      781 atacggcaaa ggtctacata ggcgcaaaat atacctttgc agagctgctg gaccaggacg
      841 aggtggcgtt taaattccgc ctgatcgtgg aacgctatat tttagcggaa aaagaggtgg
      901 atccccagga tacgctggag actcatctgt attatctgaa gccggacagt tttcttgtga
      961 agatctacga ccggatcaag gctcgggtga agatcaatat catcgaggag aaaaaaagga
     1021 ttttcggcag aggcaaaaag cgttatgtca cgaaggaatt gaacatccgg gaattagttg
     1081 ccatgacacc ggaggagaaa gaggcgaagg gtgtcgtgat tcaggaaatc tccatgagca
     1141 agctggcact ggccgcgttt tgacagatta agctcaccca cagaaatcta cgcggctgct
     1201 tgcagacagc ggagattttc tgtgagggga gcaaacgggc atgagtaaga agggcgatag
     1261 cccggattac gaatggacgt tacgatttcg agagctcgaa gagcaaagaa atcggttaag
     1321 aaagatttag aaaagaggac acatcagaaa tgagagtaga agatttatcc acatatgaga
     1381 ttatagagaa acggcagatc ccggacatta acagcgtgac ctatctgtgc cgtcacaaga
     1441 agaccggagc aagagtggca cttgtatcca atgatgacga gaacaaggta ttctatatcg
     1501 gtttccgcac cacacccaag gattccaccg gtgtagccca tattctggaa cactccgtat
     1561 tatgcggatc gaaggaattc ccggtgaagg atccttttgt ggaactggta aaggggtctc
     1621 tgaacacttt cttaaatgcc atgacctatc cggacaagac tgtatacccc gtggccagct
     1681 gcaatgacaa agatttccag aacctgatgc atgtctatct ggatgcggtt ttttacccga
     1741 atatttacaa aaacgaatct attttccgac aggaaggctg gcattatgag ctggaaggtg
     1801 acaacgagga gctgaaagtc aacggtgtgg tctataacga gatgaagggt gctttttcct
     1861 ctccggatga tgtgctggag cgggagatca tgaattccct gtatcctcac accacctacg
     1921 gctgcgaatc cggaggtgat ccggaagcga tccccgagct gacctacgaa gaattcctga
     1981 atttccaccg gaaattctat catccctcca acagctatat ctatctctat ggcaatatgg
     2041 atatggcgga gaaactgacc tttatcgatg agcattatct gtctgtctac gatgcccttg
     2101 aggtggattc cgaagtgacg gaagaggcgg catttaccac gccgaatcgt attgtcaggg
     2161 aatgtcccat cggcgaggga gaggacgagg aggagaatac ctacctgtcc cagaacttct
     2221 gcgtgggcaa tagtctggat ccgaagcttt acattgcgtt ccagatcctg gactatgccc
     2281 tgtgttccgc tccaggagca cccttaaaac aggccctggt ggactgtggt gtgggtaagg
     2341 atgtctacag catctatgag aatggtatcc gccagcccta tttcagtgtg gtggcgaagg
     2401 acacttccgt agagaaagaa caggaatttt tacaggtaac ggaagaagtg ctgaaaaagc
     2461 tggtaaagga cggcttcgac gaaaaggcat tgcttgccgg catcaattat tatgaattca
     2521 aatacagaga ggcggatttc ggttcctacc ccaagggact tatgtacggt ttacaggtgc
     2581 tggacagctg gctgtacgat gacagactgc cgttcattca cattgaggcc aacgagactt
     2641 tcgctgaact tcgagggaaa gtgaagaccg gatattttga gggtctggta cagaaatatc
     2701 tgctggacaa tacccatcgc agcgtggtga tcttgcagcc gaagctggga cttctggaag
     2761 agcaggagca gaaacagcgg gaaaagatgg cacaggtgaa agctgccatg agttcggaag
     2821 agatcgagaa cgtgaaggag accttccaga agctgaccga attccaggag agcgaagacg
     2881 caaaggaaga tctggagaaa atccctctgt taaaacggga agatatgaag aaagaggcaa
     2941 atctgccggt gaacgaagtg cggagcattg gagataccac acttttgtat catgatctgt
     3001 ttacaaacgg catcggctat ttacgtctga tcttcaaatt ggatcagatc cccggtaaat
     3061 atttcccgta tatcggcatt ttgaagggct gcctgggact gttaaatacg gagcattatg
     3121 cttatggaga tctgtttaac gagatgaatc tggtcaccgg cggcatggct gcggtaaata
     3181 atgtctatgg caagctgcag gatacggatc agtttatact gactctggaa ctgaagacga
     3241 aggtatttta tggccgcctt gcggaagcaa tcgatctgat gcgtgagatc gtaatgacca
     3301 gtgattttac ggatgccaag agactgtatg agattctggc ggagggcaag tcccggatgc
     3361 aggcacagat gacctccgga ggacacagtg tggcggccgg aagagcctta tcctacggca
     3421 gtattcccgg agctgtgagc gaagagattt ccggtatccc gttctatcgt ctgatcacgg
     3481 atctggaagc tcattttgac gagaaaaaag aagaattggt ggaaatctta cagaccttag
     3541 tgaaaatgat cttccgtccg gaaaacctca tggtggattt cgtaggtgag gaaaaagccg
     3601 tggctctttt ggatgctccg gtggaagctt tcaaagcagc actttatatg gaaaacgtgg
     3661 aaaaagaaca ttacattccg gaaacctccc ggaaaaacga aggtttctta acctccggtc
     3721 aggtcaatta cgtatgccgt gccggtaatt tccgcaagag tggactgcaa tatacgggag
     3781 cattaagagt actcaaagtc atgatggggt atgaatatct gtgggttaat gtccgtgtca
     3841 agggtggtgc ctacggctgc atgtgcagct tcggcagatc cggtgacagc tactttgtct
     3901 cctacagaga tccgaatctg ggcaagacca ttgaaactta cgagaaagct gcagatgcca
     3961 ttgcagagtt cacagcagat gaacgtacca tgacccagta tatcatcggt gcggtcagtg
     4021 atctggacgt acccatgaat ccggcggcaa agggactgta ttccctgagt gcctatatga
     4081 caggactgga taatgccact ttacagagag aacgcgacga actactggcg gctaccgaag
     4141 aggatatccg tgcattgtcc gcacatatcc gtgcatttat gcaggaggat ctgctctgtg
     4201 tcgtaggcac ggcttctaag ataaaggagg aacaggagag attcctgaag gtggaaaatc
     4261 ttttttaaaa cggcaaaagg aaagctgcga ttttgcaaaa gtggttctga atcgtaaaaa
     4321 gatcaaataa ataaagaact tttgtgatat tatttcgttt atagaggtga acaataaaaa
     4381 gtcaacagca ggcggatccg acaggctgtc aggatgccgt ccgcagaacg gagagggtta
     4441 tgaaaaaacg aaattttgaa acaaagatca ttacagggtt gctcactgcc ggcatgatat
     4501 tatcgctgac cgcctgcggc agctcatcga acggcagtta cggaatcagt gatctcatgg
     4561 atgcggatta tgctgttgct tccaaggagg cagctgcacc ggaggcgtcc gcttatgaag
     4621 atggatttgg catgacagaa gggtacgatt ccgcaggaaa tgctcaggac acaagtacta
     4681 cagtgcctga ggagactgca gccacagcag accggaaact gatcaagacc gtgaatatgg
     4741 atgtcgagac cagggagtat gataagctgt taagcgcggt agagaataaa gtaacagagc
     4801 tcggcggcta tatcgaaagt ctggatgcct ataacggcag tacatattat agttatcgtt
     4861 ctacaaggaa tgccaatttg accatccgca tcccgaagga cagactggag gagttccaaa
     4921 atacggtctc agagctgggc aatgtgacca gtcgttcgga gaatgtacag gatgtgacac
     4981 tgacctatgt ggatctgcaa agtcacaggg atgccctgca gacggaacag gagagactgt
     5041 tgcagctgtt ggaacaggcg gagagcatcg aagacatcat taccatcgaa cagcgcctgt
     5101 ccgatgtgag ataccagctg gaaagtatgg aatcccagtt gagaagttat gataatcagg
     5161 ttgactacag caccgtatac ctctatattg atgaagtaga ggtctatact ccggtagagg
     5221 aagagaccac atgggaacgt atttctgcag gctttatgga cagcttaaag agtatcggcg
     5281 agggtattaa ggaagcagcc atctggttcg tgatccatat cccctatctg atgatctggg
     5341 ccatcgtgat cgtgatcatt gtactgatcc tgaaaaagat caagaagcgc acgaagcgga
     5401 tccgggcgga ggaaaagaaa aaattcgccg aagaggaagc aagaatgcag aagactttgc
     5461 agaacacggc agagaataca gcagagaata cggtagccac acaaaacagc aatgagtgag
     5521 aatctataag ataagccgga cggaccggcg gctgacagaa aagcgtcagg gaaaaagaca
     5581 agaacaggag aaataataat gaacgaaaat ccggcataca aatccggctt tgccacactg
     5641 atcggacgtc ccaacgtagg aaagtccaca ctgatgaacc gactgatcgg acagaagatt
     5701 gccattacat ctaacaaacc ccagaccacc cgtaaccgta tccagacggt gctgacactg
     5761 gacgagggac agatcgtatt tctggacact cccggtatcc ataaggcgaa aaacaagctg
     5821 ggcgattaca tggtcaatgt tgcggagcat acattgaacg acgtggatgt gatcctgtgg
     5881 ctggtggagc ccacgaactt tatcggtgcc ggagaacgtc atattattga gcagctgaag
     5941 aagaccaaga ctcccgtgat tctggtgatc aacaagacgg ataccgtgaa aaaggaagag
     6001 atcctggcgt tcatcgatac ctatcgcaaa gaactggatt tccaggagat cgttccggtg
     6061 tccgcactga agggagacaa cacggatgtg ctgatccagt gtattttaaa atatctgcct
     6121 tatggcccgg cattttatga tgaagatacc atcaccgacc agcccatgcg tcagatcgtg
     6181 gcggagctga tccgtgaaaa ggctctgaga cttttgtccg aagaaattcc ccatggtatt
     6241 gccgtgacgg tggaaagcat gaaggagaga ggccggatct gtgacatcga ggccacgatc
     6301 atctgtgaga gagattccca caagggtatc attatcggca aaggcggaca aatgctgaaa
     6361 aagatcggct cccaggcccg cccggagatc gaggatatgc tggagatgaa ggtcaatctg
     6421 cagctttggg tgaaggtgaa gaaggactgg agagacagcg acatcttatt gaaaaacttc
     6481 ggatataatc cgaaagacat tgagatgggc gaatagaaaa acgaaatttg cagaccctat
     6541 ttttacagag gataatacac tgatagaaga gggggatgcg taagatgcaa ttcacgaatt
     6601 actggcagca atttgaacat accggcagag tggaggatta cctgaaatac ctgcagagcg
     6661 cgggaaagga ggaacctttg gctggtgcgg aatgtaaaag ccccctggtg gattgcagag
     6721 cggaggggcg tacagacctg tccgcagggg aggatggatc atgcaggaat ccctgtatgt
     6781 aacgggaatt gtattaaaac aggcaccttt cggtgaatat gacaggcgtg tatgcctgct
     6841 taccagagaa aaagggaaga tcactgcttt tgccagaggg gccagaaagc ccggtaaccg
     6901 gctggctgca gcgaccaacc cttttgcttt tggctctttt cgtctgtatg aaggacggaa
     6961 ctcttatacg atatcggaag cagagatccg gaactatttt ccggaactga tgacggacta
     7021 cgtgggggca tgctacggta tgtatttcgc ggaagtggcg gatttttact gccgggagaa
     7081 caacgatgag aaagaaatga tgaaactggt ctaccagtcg ctgcgggctt tgtgtgcacc
     7141 ggcgcttccc aatgaactgg tgcgcagtat ttttgaactg aaggccatcg ttgtcaacgg
     7201 agaatatccg ggagtaccgg aggagcgcaa actggaagaa tccacacggt atgcactgaa
     7261 ttacatagcg gagagttctg tggaaaagtt atataccttt accgtatcgg acaaggttct
     7321 tgcagaactt tcacagatcg cgtcggaata tcggaagcgt ttcatggaca ggtcgttcaa
     7381 aagtcttgaa atactgaaaa ctctatgtta aaatcggtta tggctgatgc caaaaaagga
     7441 gactcattta tgaggcagaa tagaataaaa aagtttctta cggcaggatt gtcggggctt
     7501 ttgatactgt ccctcacagg ctgcggtcag gcggcaaagc ttcccgagac tgtagagaat
     7561 acctctctgg tggtggagaa ggacggcaaa gtgacctctt atctggtgaa tacttttgac
     7621 aaagacttct acaatctgga cggccttaca cagatggtgg aggaagaggc tgaggaattc
     7681 aatgccacac atacagaggc tacagagaat cccatgaatg tgaaaacggt gcaggctttg
     7741 ggagacggag ccctggtgca ggtggtacag gagttcgccg atacggacag ttatgccgag
     7801 tacaacgaac aggacctgtt ctacgggacc agagtggaag cgctggcaca gggactcacg
     7861 gtgaatcggg aacttgtaaa cgcagcggac ggcactccgg cggattctga gaagctggac
     7921 aaggcgttgg aaaagaatca tctgatcatt acaaatgcaa gcgcatatat atactgtcct
     7981 tatccggtgc tatatatcag cgagggtgtg gtcatgggag aggatgggta tgtagatgcc
     8041 tcccaatccg atggtgtcgt tacgatactg atgaaaaaat aggctttaca gtaatacaaa
     8101 tatagagcaa gaaaggtttg gtactgacat ggaaaagaca atggagaaaa tcgtagccct
     8161 ggcaaaagca agaggttttg tatatcccgg ttccgagatc tacggaggac tggcaaatac
     8221 ctgggattac ggtaatcttg gtgtagaact gaagaataat gtgaaaaagg catggtggca
     8281 gaagttcgtg caggagagcc cttacaatgt aggtgtggac tgtgcgatcc tgatgaatcc
     8341 ccagacctgg gtggccagtg gacatctggg tggtttctcc gatcctctga tggactgtaa
     8401 ggagtgtcat gagcgtttcc gtgcagacaa actgattgag gatttctgtg aggagaacgg
     8461 cattgccatt gaaggcagtg tggacgcatg gagccaggaa gaaatgaaga atttcatcga
     8521 ggagcacaat gttccctgtc ctacctgcgg caagcacaac ttcaccgaca tccgtcagtt
     8581 caacctgatg ttcaagacct ttcagggggt taccgaggat gccaagaaca cggtttattt
     8641 aagacccgag accgcacagg gtatttttgt taacttcaaa aatgtacaga gaacttcccg
     8701 caagaagatt cccttcggta tcggacagat cggtaagtcc ttccgtaacg agatcacacc
     8761 cggcaatttc actttccgta ccagagagtt tgaacagatg gagctggagt tcttctgcga
     8821 gcccggcacc gatatggagt ggttccagta ctggagaggc ttctgccgtg actggctgct
     8881 gtctcttggc atcaaggaag acgagatgag acttcgtgat cactctcctg aggaactgtg
     8941 cttctacagt aagggaacca ccgatattga gttcctgttc cccttcggat ggggcgaact
     9001 gtggggtatt gcggacagaa ccgattatga tctgacccag catgccaata cctccggcga
     9061 ggatatgact tacttcgatg atgagaagaa ggaaaaatac atcccttacg ttattgagcc
     9121 ttctcttggt gcagacagag tggttctggc attcctgtgt gcagcttacg acgaggaaga
     9181 gttagagggc ggcgatgttc gtactgtgct tcatttccat cccgcactgg caccggtaaa
     9241 gatcggtgtg ctgcctctgt ccaagaaact gaacgagggt gcggagaaga tctacacaga
     9301 gctttccaag aaatacaact gtgaatttga tgacagagga aacattggta agagatatcg
     9361 tcgtcaggac gagatcggaa ctcctttctg cgtaacttac gattttgatt ccgagacaga
     9421 tggctgcgtt accgtacgtt tccgtgattc catggagcag gagcgtgttg ctatcaaaga
     9481 tctggatgct tactttgcag ataagtttac attctaagat cactattcag gagcagaaag
     9541 atttttctgc tccttttcaa tatctttttc tattggtacg aaaactttga tacttacagt
     9601 ggaggggaca gcgtggaacc gatcgaagtg tttcagattc tgggaatcga acagacaaaa
     9661 gacgaaaggg cactgaagaa tgcctatcgt gacaagctga cggtaacgaa tccggaggat
     9721 gatccggaag gcttcaagcg actgcggatg gcttatgagg aggcctgccg ctatgccgga
     9781 acgccggatg cagaagagaa cgaggaggcg gaaccgacgc tggaggacga tactccggct
     9841 ggacagtggg tacgtggtgt tcggaaggtc tatgagaata ttacggaccg ctgtgatgtg
     9901 gaaaaatgga aagcgctttt tgaggcggat gactttcttt ctctggagga agaggaaaac
     9961 tgcaccactt atctgcttcg ttttctgatg gaacattata agctgccgac cgcaatctgg
    10021 aagcttctgg atgagaaaat ccacattgta cagaatgcgg gggccttcag agaacgcttt
    10081 ccggcacagt ttgtaagcta tatggtacat aagtgcgaat ccggggaaga agtggatttt
    10141 tccgagttcc gtggtgcgga agatgcggat tacgatcagt ttctgcaata ttatgacaga
    10201 gcttatcagg cgctgcagga gaaaaaatta caggaagcgg agcagatgat cggatgtggg
    10261 gatgctctgg ggattaccca ccctgttatg gagatctgcc gtgcgtccct ctatgaagga
    10321 aagggacaga ccgcagaagc cattactttg ttaaagaaac tgtctgcaaa atatccggag
    10381 gatgatctga tcgcttacaa tacggcggaa atactgtgga gaaatgaagg gcgcgtagaa
    10441 gcgtctgcta tttatgagag tcttcgggag aagctgccga aacactatat ggcgaacctg
    10501 cgtctgacgg actggtatta tgagcagggg aactataaag aggcaaaaaa atgtgcggaa
    10561 gagattcttt ccgtaggcgg agacgacaca tttctggaga cactgcagaa gatcaacacc
    10621 gaactggaac gggaaatgaa acgggaatat gcgcagaacc atgatcccgg tctgggactg
    10681 gatctgggct ggtgttatct gcaggatggc aggaccgatc tgggaatccg cacggtcagg
    10741 gagctggaag caaaagttcc tgcggacagg cgtgaggaat atctgggact tatgacaaaa
    10801 ctgctggcag aagcggcaga atatgagaag gcaatttcca cggcagccaa atgggaagaa
    10861 agcctggaga aaaagatcct ccgggatacg gatgccgaag aggaggaaaa agacagagac
    10921 cggatccgac agtcacatat gatccggatg cagtgctatc gggcttatgg ctatgtacag
    10981 ccggaaaaat tcgccgaagc cattgcggaa gcggaaaaag ccgagaccgg aacaccgaag
    11041 gatatcggta tgctgatcga aaaggcacag atctatgtgg agatggaaga gtatgaacgc
    11101 tgtgcggaga tatcccagaa actgatcggg gattatcagg tttacgctgc atatgccaac
    11161 gaactggaag cggcgaagag gcagtggaat gcagcagggg tgatccagaa cggcagagcc
    11221 tgtctaaatt attttccaac ctatgtcaag gcctatgaaa tgatggcaaa ggtgtacatg
    11281 gatctggcac atccggagga actgaaggaa ctgttacaac aggcaaagga taataatgtc
    11341 aaaagtgtga tcctggatgc ctatgaatgg cagctgacca atacggggct tccccaggaa
    11401 cagatgaacg acgccataga taaattccgg aaggaatatc tgagcaaact ggaagcgggc
    11461 aagaccatat tttaccggag aggacttccg atcattacgg aatatctgta tgcctaccct
    11521 tgtgaatttt tactggtgga gcggggagat tttcataatg cggcaggctg tctggaggcg
    11581 gcaaaagcgg attatgaaaa ggcgctttcc acgaacccgg ccaatccttt tgcctggaag
    11641 ggactggcac ggatccacag atgccgcggc aaatacgatg aagccatgat ctgcctgaga
    11701 aaatgtgtat ttttctatga aaagagcgga acacagaatc atgcctggcc ggaactgatt
    11761 gcggaacagg cagaaattta ttttttgctg ggtaatacgg aaatggcgga acagttttac
    11821 cggagatacc gtgagatgac gggaagtgcc ggagaacggg accggaaccg tatgagggat
    11881 ttcgccagat gtctggcctg caatgacaag actacggagg gattaaaagt tctggagaag
    11941 gcattcgtca atgtactgga tgcggcagga gaaaagctgg atctgtgtgt ctggtgcggg
    12001 gaaaagacga tcgccggaaa tattctgaca tcttggccgg agaagatcga actgcttggg
    12061 aaaaataccg ggaataccca ggagtatttc gaagattact ttttccatct gggatggtat
    12121 gggctgatct gtggttccgg aaaagtggcc atcaagaata tggataaggc actgatcttc
    12181 cataaggagg atctcagcaa aaaggatgac attgcggacc tgattctggc atgcatcctg
    12241 tacggagata agaagaaagg tgctgactat gcacaggcgc tgaaagcctg catggaaaga
    12301 gaagataaaa gcggcaagga tgtgtatctg aaatatccga agcttcggat cgtacacgaa
    12361 tatctggcgg gatattatac ggccaccgat gaggaacagg atacgctcct gcaactggac
    12421 aaagactgtt ccttctgcca tggctgtgta catcccgtct gcgaggaact ggagatgata
    12481 cggatactgc agatgctgaa aaagggccgg gagaaagaag cactggagcg gctgaaagag
    12541 cagatgcagg aacatcccgg aatgggattg caggccatct ggcaccggta tcattcggaa
    12601 caggtgacga aggatacgga tcctgccgta gccgcttttc acaaggaaaa gccgcagccg
    12661 gagaaacggg gattctggca gaggctgttc gggaagaagt aaaaaattaa aatgaagaga
    12721 gaagaaaaca tatgatagtc ggaatcgatt tgggaaccac caacagtctg gtggcagcat
    12781 ttacggagga aggacctgtg atcatcccca accgtctggg aaaacatctg accccctctg
    12841 tggtcagcgt ggatgagaac ggcaatgtct atgtggggga gacggcacag gaacgcagaa
    12901 acctctatcc cgacagtgtg gcgcaggcat ttaagcgcag catgggaacg gatcgcactt
    12961 atgacctgtc cggcaaaaag ttccgcccag aagaattgtc cagcatgatc ctgcgttatc
    13021 tgaaagaaga tgcggaagcc tatctggggg aagaagtcac agaggccgtg atcagtgtac
    13081 cggcctattt tgacgacaag agaagaaaag ccaccaagcg ggccggagaa ctggccgggc
    13141 tgaaggtgga gcgtatgatc tcggagccta ctgcagcggc ggtggcttac ggcctgtacg
    13201 aaaaagaaaa agacaccaga tttctggtat ttgacctggg cggcggaact tttgatgtct
    13261 ccattctgga gctgtatcac aatattctgg aggtgcgggc ggtagccgga gacaattatc
    13321 ttggcggtga agatttcacg gaagtgatgt cgaaactttt tctgcaaaag acaggactgc
    13381 attataagga tctgtcggag aaagaacagg tgcgtctcta taaaaaggca gaagaagcca
    13441 agcggggaat cagtgatcag actgcggtga ccatggaact tatgctgggt gaagagaaca
    13501 aaacggcaga gatcacctta aaagaatatg aggaagagtg cgaagaactg ctaatgaaga
    13561 tcagggaacc ggtgaagaag agtctggcgg atgcaggact gaagctgtcg gacatcgatg
    13621 aggtattgct gatcggcgga gccaccagac tgtcggtggt cagggatttc ctgatcaggc
    13681 tgttccgcaa attcccggat acaagactga acccggacga agccgtggca ctgggcgcgg
    13741 cgatccaggc ggccatgaag gaacgccggg aggaggtaaa ggaagtcatt ctgacggatg
    13801 tatgctcctt tacattggga acggaagtcg tggtggaata cgaggaaggc aaatttgaag
    13861 acggccgttt ctgtcccatc attgagcgca acaccgtgat ccccgccagt catacggagc
    13921 ggttgtacac tgtcagagac aatcaggaca aggtgcgcgt ccgggtactg cagggagaga
    13981 gccgtttcgc cagaaataat ctgtttctgg gagaactgaa cattgatgtt cccaaggggc
    14041 ccagaggcag tgaagccgtg gatgtgactt acacctatga catcaattcc ctgctggaag
    14101 tggaagtcaa ggtggtatcc accggactga cccagaaaat gatcatcaag ggacaggaca
    14161 accagatgac ggatgatgag atccagaagc gaatggaaga attgtcctac ctgaagatcc
    14221 agcccagaga cctggaggag aaccgtctgg tgcttctcag agcggagcgg atgtatgagg
    14281 aagcactggg agaccggaga aaagaactgg atcgttatat cactgttttc gaggctgcat
    14341 tgaaaaaggg caaaaaagag gagatcgagg aggccagaga ggcattgaac gagatcctgg
    14401 aagacgagga tgagtgatgt tttatccttg acgcggcgaa acgataacat tataatgtag
    14461 aaatgtaggt ttattttccg aaaggtgtaa tttttagaca aggagaccga gagaatgaga
    14521 caattcactt ctaatgagct gagaaaagct tggaaacaat tttatataga ccgtggccat
    14581 gtggatgtgg gagcagtatc cctggtatct gacggatcca ccggtgttat gttcaacgta
    14641 gcgggcatgc agcctctgat gccttatctg ttgggacaga agcatcctct gggtaccaga
    14701 ctctgcaatg tgcagggctg cgttcgtacc aacgatatcg attccgtagg tgacaagagc
    14761 catgtgactt tcttcgagat gatgggaagc tggagcctgg gagattattt caagaaagag
    14821 cgttgccagt ggagtttcga actactgaca caggtattcg gatttgatgc cgaccatctg
    14881 gctgctactg tatttgccgg tgacgagaat gcacccagag atgaggaagg cgcacactac
    14941 cgtatcgctt ccggtttcaa gaaggagaat gtatattatc tgcctgccga tgacaactgg
    15001 tggggactgg aatacggccc ctgcggtccc gacagtgaga tgttctatat tgcagacaga
    15061 ccggactgcg gaccgaactg cggacccggc tgtgactgcg gtaaatacac ggagctgggt
    15121 aatgatgtat ttatgcagta tgagaagcat catgacggtc atctgacccc cttaaagcag
    15181 aagaacgtgg ataccggttg gggcctggag cgtattctgg cctttttaaa cggtaccaga
    15241 gatgtatacc agacggatct gtttgctccg gtcattgctt atatcgagga ggcttccggt
    15301 gtgaaataca atgcggatga aaagctgacc agatccatgc gtattctggc agaccatctg
    15361 cgtaccagtg taatgctgat cggtgacgaa gcaaagctgc ttccttccaa taccggtgcc
    15421 ggttatattc tccgtcgtct gatccgtcgt tcggtaagac acggacgtac cctgaatatg
    15481 actacagagc agctgctgca cattgctgcc atgtacattg atgagatcta tgcagaaagt
    15541 tatcctctga tgaaaaagaa cagagagttc attctgagcg aactgcagaa ggagatcgca
    15601 cgtttcgaga gcactctgga gaacggcatg aaggagttgc agaagattct ggagcagaag
    15661 agaagtgaag gtaaaaagga gatcgacggc aagtccgcat tctacttata tgataccttc
    15721 ggattccctc tggaactgac tgtagagctg gcacaggaag agaatctgac cgtagatgag
    15781 gaaggctttg ccgctgccat ggaagagcag aagcagaagg caagagaagg acagaacttc
    15841 tcccagaaga ttaccaccgc agcaggtgta ttcgatggac tggatgagaa ggtcaccagt
    15901 gaatttgtag gttatgataa actgaccgca gaaggaaagg tagtagcact ggccaccgag
    15961 actgaactgg taaacacctt aaagataggt gagaccggta cactgatcac cgatgtgact
    16021 ccgttctatg ctaccatggg cggacagaag ggtgactttg gtgtgatcag caccgaaaac
    16081 ggtacttttg aagtgactga gaccgtgaag atcgcaggcg gacgcatcgg acatgtgggt
    16141 aaggtcgtat ccggaacagt tactgtggga gacaaggcag agctggcagt ggataccgac
    16201 aacagaaaga gcgtatgtaa gaaccattcc gcaacccatc tgttacagaa ggcactgcag
    16261 atcgtactgg gtgatcatgt agaacagcag ggttcttacc aggacggcgc aagaacccgt
    16321 tttgacttca gccacggaca ggccatgact gcagaagaga tcgctaaagt agaggctctg
    16381 gtcaacgaga agatcgcaga ggatattgca gttgttaccg atatcatgag catcgaggat
    16441 gcgaagaaga gcggagctat ggctctgttc ggagaaaaat acggcgatac tgtccgcgtg
    16501 gtatccatgg gagacttctc gaaggaactg tgtggtggta ctcatgtaaa gcataccggt
    16561 gagatcggtt acttcaagat cctgtccgag agcggtgtcg ctgccggcgt cagaagaatc
    16621 gaagcactga ccggtgccaa cgtgatggca tactatcagg ctatggaaga gaacttcaat
    16681 aaggcagccg cagccgcaaa ggcagccccg gcagctctga ccgagaagat ccagcatatg
    16741 caggcagaac tgaaggctct gaatgctgag aacgaatccc tgaaagccaa gatggcacag
    16801 tccgcactgg gcaatgtcat ggatcaggca gtggaagtca agggaacaaa gcttctggca
    16861 accagcgtag acggtgtgga tatgaatggc ttgcgagacc tgggtgacca gctcaaggat
    16921 aagcttggtg aaggcgttgt cgtactgctc tctgcacagg atggcaaggt caatatgatc
    16981 gccatggcaa cagacggtgc tgtaaaggcc ggtgcccatg caggcaacct gatcaaggga
    17041 attgccgcac tggttggcgg tggcggcggc ggacgtccaa acatggcgca ggccggcggt
    17101 aagaatcccg caggcattcc cgcagcgatc gcggaagcgt ccaaagtact ggagggtcag
    17161 ctcgcataat ggaacgagag aatctgaaat cccgtttagg ctttatactg ctgtctgccg
    17221 gctgtgccat tggtatcggc aatgtatgga agttcccgta catggccggc cagggcggcg
    17281 gtggcgcatt tgtattgttt tatctgatct tcctggtgat ccttggtctg ccgattatga
    17341 ccatggagtt cgctgtggga agagcaagta agaaaagtcc cgtaaaggct taccaggcac
    17401 tggagaagcc gggacagaag tggcatatcc acgggtattt cacgctggca ggctgttatc
    17461 tgttaatgat gttctatact accgtagcag gttggatgtt acactatttt tatatgacgg
    17521 ctaccggtaa actttccgct ctttccgcag atgcggtagc ggatcagttt acccggatgc
    17581 tggcagatcc aggggtcatg atgttctgga tggtactggt ggtagtcatc ggtgtcgtca
    17641 tatgtgccgg tggcctgcag aacggtctgg agcgtgtgac gaaagtcatg atgatcgcac
    17701 tgcttgccat catggtagta cttgccatca acagtttctt catggcggga gcaaaggaag
    17761 gtctgaagtt ctatctggta cctgattttg gacgtatgca ggaggttggt gtggtatcta
    17821 cactggtagg tgccatgaat caggcattct ttaccttaag tcttggcatt ggtgcaatgg
    17881 cgatcttcgg aagctacatc ggcaaggatc attccctgat gggtgagtcc gtcagagttg
    17941 tggtactgga tactttcgtg gcaattaccg caggactgat cattttcccc gcctgcttta
    18001 cctatggggt ggatcagact tccgggccca gcctgatctt tattactttg ccgaatattt
    18061 tcgcaaatat gccttacggc cgcctgtggg gaagcctgtt cttcctgttc atggcgttcg
    18121 cagctctgtc tacagtactg gcggtatttg aaaatatcat ctgctgtggt atggaactga
    18181 ccggtgccag ccgtaagaaa tcttccctgg tgaacctgtt cctgatcatt gtactgtccg
    18241 taccctgcgt actggggtat aatgtatgga gctgggatgg ttttgctgtc ttcggcggag
    18301 cagtgctgga ttttgaagat ttcctggtaa gtaatctgtt cctgccgtta ggatctctgg
    18361 tatatctgtt gttctgtgtg accagatacg gctggggatg ggagaactat aaggcagaag
    18421 taaataccgg caaaggctta aaagtccacg actggatgcg cggttatctg acctatggac
    18481 tgcccctgat cgtgatcttc atcttcctgt ttggtatcta cgataagttt tttaaataag
    18541 attttgaaga tccagtagct tgcacaagaa taaggacaag cagactctaa gcaacccgtc
    18601 ggtaccatag cgtcccaagg gacgcattgc gagggtattt tccacgctta tgtaccatta
    18661 cggttaagcg tccgtaagcg aatcgtgcga aagaaagcac gcgcgggtct gcggttctta
    18721 ttcttgtgtg agctacgggg aatattatat ataaaaaata aaaaaagtag ttgacgaata
    18781 cattttttat gctataatat caaatgtgtg tagaacaact gcgcattaga ataacccagt
    18841 cagtcatgtt acttgaaggg actgaaggga ggtcgggtgt aagatgtcaa acgtaatcgt
    18901 aaaagagaac gagactttag atagcgcttt acgtcgtttc aagagaagct gcgctaaggc
    18961 tggtatccag caggagattc gtaagagaga gcattacgag aagcctagcg taaaacgtaa
    19021 gaagaagtct gaagcagcaa gaaaacgtaa atataactaa tgcaaaacaa cagtcctcgg
    19081 cgtaatcacc ggggactttg tacttatgag ggaaacaaaa tgtggaatta tgaaatactg
    19141 ggaacagata tctatcatct gttagcagcg tttgtattgt acagtattct tggctgggca
    19201 ttggaatcta cctatatgtc tttctgcaat cacaagctga ccaaccgtgg ctttggcaaa
    19261 ggcccctttt gcccgatcta cggtttcggc ggagtgctgg gatatctgat tttaagtccc
    19321 ctgcgtggga aactggtaaa gctgtatttt cttggagcca ttctggccac cacttttgaa
    19381 tatctggtgg gaatcggtat gatccgtttc ctcggagaac tctggtggga ttataacaac
    19441 aagcccttca attaccgtgg tatcatctgc ctggaaagta ctgttgcctg gggcttttat
    19501 gcaataggaa tcgtacagtt cgttcacgat gccatgtatc acattattga cagagtacag
    19561 atccaggtgg gtatccgttt gattcaggta attctggcga ttgtagctgt ggattatgtg
    19621 gtccagctga tcaaagtatt tcacatcgat gtgcgcagta agggtgaagc cctgaaggaa
    19681 cgctgtcagg cactgatcac cagactgtag tgttccggac ggttggatta caggtatata
    19741 caaggccata cgaccatatc ttttagaaga aaggataagg gagtgtggct tttttatgca
    19801 aaaaaacagg aaaaggattt tcacatggat cttacttttt acggtcagca tacaggtatt
    19861 ctggtgggac gggatcagtg tatctgcgga gccggcggca atcgaagctc ccagtgccgt
    19921 gcttctggag agctctaccg ggaaagtggt ctttgaacag aatgccagag agcgcagaag
    19981 tcccgccagt attaccaaga tcatgacatt gctattgaca ttcgaggcat tggatcaggg
    20041 gaagataaaa ctggaggatc cggtgaccgt cagtgcttac gccagttcca tgggaggctc
    20101 ccaggtattc ctggcggaga acgaggtaca gaccttggag accatgatca aatgtattgc
    20161 tgtagcttct ggcaacgatg ccagtgtggc ggtggcagaa tacatagcgg ggagtgaaga
    20221 ggctttcgtg gatcagatga atcaaaaggc ggcagaactg ggaatggtgg atacacattt
    20281 cgaggattgt tgcggactga cggattccga cggacactat acgacagcca tggatgtggc
    20341 cattatgtcc agagagctta cggtgaaata tccccaggtc tttgagtata ccggtatctg
    20401 gatggaagac atcacccatg agaccagaca gggcagctct acatttaccc tgaacagtac
    20461 caacaagctg ttgaaacagt accagtgggc tacgggactg aagaccggat ccacctccaa
    20521 ggctaaattc tgcctgtctg ccactgccac gaaggatggt attgacctga ttgccgtggt
    20581 catgggagcc ccggactaca aggcacgttt taaggatgct cagacactgt taagttacgg
    20641 tttcaatgtc agtgacctgt atctggatga gaatacggat gtattggagg accttcggat
    20701 agaaggtggc gtggaagata cggtacctgt cagataccag agcgaattcc ggtatctgga
    20761 tacggaaggc aattcgctgg acggagtgaa gaaaaccata gaattaccgg aggaggcaca
    20821 ggcgcctgtc gcaaaggggg cagaggcagg cagagcggtc tatctgttga acggagtgga
    20881 gatcggaagc gttccgatcc tgtatgaaaa tgatgtggcg aaagcggtat acaaggacta
    20941 cctctttaaa ataatggaat tttatctttt gtaagcccga aaaatatgtt atactataga
    21001 acatattggc aattaactgg aaatagacca tgggggcaaa gaatttggat gtggtgatta
    21061 ccgtgatcgc agtgatcctg gtggttcttg tatggataat gctgtacgac agcaatcgtt
    21121 ttgtggtacg gcattatttc ctgcgggatc agagaatcaa aaagcctgtt aaggcagtgg
    21181 ttctggcgga tctgcacaat aagagatacg gaaaagaaaa tgagcgcctg ctgcaggcca
    21241 ttgatgagat aaggccggac atgatcctga tcgccggaga tattctgacg gcaaagccga
    21301 aggcaagtct ggagacggcg gtggacctgc ttacgaagct tgccgggaaa tatccgatct
    21361 actacggcaa cggcaatcat gaacaccgtt tgaaactgta cccggagaac tacggagata
    21421 tggcggagcg gtacgaggag gccctgcaga agatcggaat ccggaggctg gtgaatgaac
    21481 ataccgttct ggaagaatcc gggatctgca tttacggatc ggagatcgac aagctgtatt
    21541 acaagcgctt cgggatccag cccatggatc cggagtatct gaaaagtctg ttgggacaac
    21601 cttccgcaga aaagtatacg atcctgatcg cccacaatcc ggattatttt ccgaaatacg
    21661 cggactgggg agcagatctg gtgctggccg gtcatgtcca cggcggtatg gtgagagtcc
    21721 ccatctgggg aaaaggcgtg gtgtctccca atgtgagact ttttccaaaa tatgatggcg
    21781 gagagttcac actggggaaa accagaatgc tgctcagcag aggactggga atgcatacga
    21841 ttccaatccg gctgttcaat cctggagagg tcctggaagt ggatctgctg ccggggggag
    21901 aagaggccgg tgggagtgat gaggggaaat aggagtttta ttacatggca atatctgtaa
    21961 aactggaagc atttgaaggt cctctggatc tgttgcttca tttgatcgag aaaaacaaaa
    22021 tagacatcta tgatattcct attgtggaga tcacggccca gtatctggat tacatcaggc
    22081 agatgcaacg ggaagatatg aacgtcatga gtgaattcct ggtaatggcg gcaacactga
    22141 tcgatatcaa gtgcaaaatg ctgctgccca aggaagtgaa cgaggacggc gaggaagagg
    22201 atcccagagc ggagctggta cagaagctgt tggaatacaa gatgtacaaa tatatgtcct
    22261 tcgaactcag agaccggcag gtggatgctg ccagaaatat gtacagagaa cagagacttc
    22321 cgaaggaagt ggaagcctac agacagccca tcgattacga agagctgatc ggcgatatga
    22381 acttgaacaa gcttcatgaa atattcaaat ctattgtgaa gaaacaggag gacaagatcg
    22441 accctgtccg cagccagtat ggcaatatcg aaaaggacga ggtggatatg gacatcaaga
    22501 tgctgtatgt ggaagcttat gccagagaac ataagacctt cagtttccga aagcttctgg
    22561 agaagcagaa cagcaaaatg gaggtcatcg ttactttcct gatcattctg gagctgatga
    22621 agaccggcaa gatcaacatc agtcaggaaa atatattcga tgatattatg attacatcta
    22681 acgtttaaga tacagtgagg tcggaacatg gaacgcagta aagcagaagc agtaatagaa
    22741 gccatccttt ttaccatggg ggattccgtg gagatcagca gacttgcgga agcgatcgaa
    22801 gaggatgtga agaccacaaa atccatttta aaagatatgg cggcgcggta tgaggaagag
    22861 aaccgtggca tcggactgac acagtttgac aattcggtac agctgtgtac caagtcggat
    22921 atgtatgaat acctgatcaa gatcgccaag actccccgaa agatgacatt gacggatacg
    22981 gttctggaga ccctgtccat cattgcctat aagcagccgg taacaagact ggagatcgag
    23041 agagtcagag gggtcagctg cgatcatgcc atcaataagc tgttggaata cgatctgatc
    23101 acggagctag ggcggctgga tgctcccgga agacctctgc tgttcggcac aacggaacag
    23161 ttccttcgct gctttggtgt gaagagcctg gaggaactgc cggaactgaa cccggtgcag
    23221 gtggaggaat tcaaacagca ggcggagcag gaagtacagc tcacattaaa catataatag
    23281 aataggacaa agaatcagcc tcataataat catagtaata agggactctt ttgggagtga
    23341 tgattatgcg aggctttttt tgtgcccttt tcgccggaat tctgtgcctg gaaagtatag
    23401 gcggattctg tcggcagggg tatgtcagcg agggaatccg ggaacaggct acgcaccttt
    23461 ttaatgaaac agcggaaaag gaagccgggc agctgcagga ggatttacac ctgtatgcac
    23521 aggcggcggt gcttctggat gccgactccg ggcgggtact atatggaaaa aatgaagaga
    23581 ctcccatggc aatggccagc accaccaaga tcatgacctg cattctggtg ctggaaaatg
    23641 caaaggtgga ggaagaggtc agtatttccg cctatgcagc cactatgccc aaggtaaagc
    23701 tctatgtgaa aaagggggag cattatacgg taagagaact gctgttttcc ctgatgctgg
    23761 aaagccataa tgatgctgct gcggcactgg cggaatatat cggcggaaag cttctgggtg
    23821 agacgaaaga ggcttcggag catactacag aagagagtaa ggcagcatta catagatttg
    23881 cacaggcgat gaatgaaaag gcggtggaga tcggctgcga ggatacctgg tttatcactc
    23941 ccaacgggct ggatgccaca gagacattga cattacctga cgggagtatt ctggaaaagg
    24001 agcaccatac cacagcaaag gatctggcct gtattttacg gtactgtatc agagagtctt
    24061 cccagagaga tctgttccgg gagatcaccc ggacacagga gtattgtttc acggaaaacg
    24121 gacgctcctt tcagtgccac aaccacaatg cctttttgca gatgatggac ggagctttta
    24181 ccggcaagac cggatttacg aacaaggcgg ggtactgcta tgtgggagca ctggaacggg
    24241 atggaaaatg tatgattgtg gcacttctgg cctgtgggtg gccgaatcat aagacctata
    24301 aatggagtga cagcagagag ttgtttggat atggactgga aaattttgtt taccggaagt
    24361 tcggagagga agaattttcc ggacgggaac agtaccttat gccgatcccg gtgacggagg
    24421 cacaggatga ggaattgaca ggagaagtgc agctggcggt ggaactggat ccggaggcgg
    24481 aaggagcgga aggcctgctt atgcgcgcag acgagaaagt ggagattctc taccggaaag
    24541 agaaagaact gaaggcaccg gtaaaggaag gaacgacggt gggagaaatc tgttatctgg
    24601 tggatggcga aatctggcgc agggagcggc tgtatgctgc ttggacagtg gaggccgtgg
    24661 atctttcctg gtgcggcaga cagatcctga aacgttttct ggtagcattc ccgtagggat
    24721 tgcagaaaaa tggaatggaa aaaatgacgg ggaaattgtt caaaatgagg tacaatgtat
    24781 aaaaaatgta aaaatctgca attcgttctt tgcgagttgc agattttatg ttatactaca
    24841 gactgttaat catgggggca tttgtgcttc tatattaata aacgttttct attatatttt
    24901 attatattat caacggaggg ctgaaaacat atgagtacta cttcaaaagc gagtcaaaga
    24961 atagcagctt tactcgatga caacagtttc gttgaaatcg gcggacttgt aaccgcaaga
    25021 gctacggatt tcaatctgaa acccaatgaa actccttctg acggttgtat taccggctac
    25081 ggagtgatca atggtaatct tgtatatgta tatagccagg acgcttccgt actgaacgga
    25141 accatcggcg agatgcatgc aaagaagatc accaatctct acgatctggc tatgaagacc
    25201 ggagcacctg tgatcggact gatcgaatca gcaggtttaa gactgcagga agcaaccgat
    25261 gcactggctg catttggtga gatctattta aagcagacca tggcttccgg cgtgatccct
    25321 cagatcactg ctgtattcgg aacctgtggc ggtggcttag gactgttccc taccatgacc
    25381 gatttcactt tcatggaaga gaagaatgcc aagctgttcg taaatgctcc taacgctctg
    25441 gacggtaatg tgatcaccaa gtgtgattct tcttccgcta aattccaggc agaagagagc
    25501 ggtattgttg acgtggtagc tgacgaagca accattctgg agaaggtaag agagctggta
    25561 agcttcctgc ctgccaacaa cgaggatgat gcaagcttct tagaggattg cactgatgat
    25621 ctgaaccgtg taaatcctga gatcgcaggc tgcgtcggcg acacatccgt tgctctgtcc
    25681 attctggcag atgacaacaa cttcttcgag gtgaaggccg gatatgctaa gaacatggta
    25741 accggtttct tacgtctgga tggcgttacc gttggtgcgg tagctaaccg ttccgagatc
    25801 tgcgacgaag aaggcaaggt agcagagaag ctggatgcag ttctgactgc agagggctgt
    25861 gagaaggctg ctgagttcgt aaacttctgt gatgctttcg gcattcctgt actgaccctg
    25921 accaatgtaa agggttacga agcaactctg gcttccgaga aggctatcgc aaaggctgct
    25981 gcaaagctga cctacgcatt tgccaacgct accgttccca aggtgaacgt tgtgatcggc
    26041 aaggctctgg gaaccgctta tgtggtaatg aactccaagg caatcggtgc ggatatcacc
    26101 atggcatggc cggatgcaca gattggtgct atggacggca agctggctgc caagatcatg
    26161 tatgatggcc agggcgctga tgtgattaat gaaaaggctg ctgagtatga agcacttact
    26221 ctgaatgtaa ccagcgcagc caagagagga tatgtggacc agatcgtaaa tgctgctgat
    26281 accagaaaat atgtgatcgg cgcattcgag atgctattca ccaagagtga agaccgtcct
    26341 gccaagaaac acggaactgt ataagaaagg gtgattgtaa aatgaagaaa tttttaagct
    26401 tagtatgtat gattacctgc atctttggtt tgaccgcgtg cggcagtgag gagacctata
    26461 cggattatga gcagcgcaaa atggacactg caatccagat agctacccag tatgtgattc
    26521 cttctctgga gaattttgaa gacgaagctg cactggagag cttctctgag tataccgctg
    26581 atgaagtggc atatatggta caggaaaatg taggtatcac cgttgatggt tatgcataca
    26641 aaactgctat cgagtccttc aactccgcta aaaagtccat tggcggcatt accgcagtcg
    26701 gtgatgcaga agctgccatc gatgatgatc agatcattgt acatgtagat gtgaccggtg
    26761 ccaaacagaa tgcacaggca gaagtgatct tcaccaacga tatgttcctg agcatggagt
    26821 ctgcagcgct gaaccccgtg gagtccatgg gtggcctgat gaccaaagct gcactgaaca
    26881 ctctgatcgg tatgggaact gtattcgtaa tgctgatcat gatctcactg atcatttccc
    26941 tgttcaactt cattccgaag atccaggctg cattcagcaa gaaggataag gaggaagaag
    27001 caaagaacgc aggtattgat aaggctgtaa ctcagatcgc tgcacaggaa gaacccgagg
    27061 cagatgacac tgagctggtc gctgttattg cagctgcaat cgcagcaagc gaaggtgcgg
    27121 caagcactga cggatttgta gttcgttcta tccgcaaagt aagataatcg taaaaacgtt
    27181 aggaggatat taaaatgaaa aattatacca ttaccgtaaa tggcaacgta tatgatgtag
    27241 tagtagaaga gggcgcatct accggagcac ccgtagcagc aaaggctccc gcagctccca
    27301 aggcagcacc caaggctccc gcagcagctc ccaaggcaag cggcgcagca ggcagcatca
    27361 aggttgaggc cggtgcagct ggtaaaatct tcaagttaga ggcaagtgtt ggacaggctg
    27421 ttaagaaggg tgatgctgtc atcattctgg aagctatgaa gatggagatc cctgtagtag
    27481 ctcccgaaga cggcactgta gcaagcattg acgttgcagt tggtgatact gttgaggctg
    27541 gtgcagttct ggcaactctg aactaggcat tttacatcag ctctataaac aacaggaggt
    27601 catcaaatgg aatatatagc tagtacgctt aacaacctga ttcatcagac agcgtttttt
    27661 aacctgacct ggggaaacta tgtcatgatt ctggttgcat gctttttcct ttatttagct
    27721 atccgtcatg agttcgagcc tttgctctta ctcccgatag cgttcggtat gctgttggta
    27781 aacatttatc ccgatatcat gctgcaccct gaaaatgcag caaacggtgc cggcggctta
    27841 ctgtattatt tctacaagct ggatgaactg gcaatcctgc cttccctgat cttcatgggt
    27901 gtaggtgcca tgacagactt tggtcctctg attgcgaacc ccaagagctt ccttctggga
    27961 gctgctgcgc agtttggtat cttcgcagca tacttcggag caatctggct gggattcaac
    28021 gacaaggcag ctgcagcaat ctccatcatc ggtggtgctg acggccctac ctccatcttc
    28081 cttgctggta agctgggaca gacggcgatc ctgggaccta tcgcagtggc agcatattcc
    28141 tacatgtcac tggttcccat tatccagccc cctattatga agcttctcac tactgagaaa
    28201 gagagaaaga tcaagatggg tcagcttcgt cccgtatcca agctggagaa gatcctgttc
    28261 cccatcgttg ttaccatcgt tgtatgtctg atccttccca ccacagctcc tctggtaggt
    28321 atgctgatgc tgggtaacct gttccgtgag tccggtgttg ttcgtcagct gactgagaca
    28381 gcatccaatg ctctgatgta catcgtagtt attctgctgg gtacttccgt aggtgctacc
    28441 accagtgcag aagcattcct gaatgcagat accttgaaga tcgtagctct gggtctgatc
    28501 gcattcatgt tcggtaccgc cgcaggtgtt ctgtttggta aactgatgtg tgtattttcc
    28561 ggtggcaaga tcaatccttt gatcggttcc gcaggtgtat ccgcagttcc tatggcagca
    28621 cgtgtatccc agaaggttgg tgcagaatcc gatcctacca acttcctgct catgcatgct
    28681 atgggtccta acgtagcagg cgttatcggt actgccgtag cagccggtac attcatggct
    28741 atcttcggtg tattttaaag cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28861 nnntatagaa aatattatta tggaggatat tcgtaatgtc agaaattgaa aagaaaccga
    28921 ttaagattac ggaaactgtt ttacgtgatg cacatcagtc tctgatcgct accagaatgc
    28981 cgaccgaatt catgcttccc atcgttgaca agatggataa agttggttat cattccgtag
    29041 agtgctgggg aggtgctacc tttgatgcat cccttcgttt cctgaaggaa gatccctggg
    29101 acagactgag aaagctgcgt gacggcttca aaaacaccaa gttacagatg ctgttccgtg
    29161 gacagaatat tctgggttac agaccttatg cagatgacgt ggtagattac ttcgtacaga
    29221 agtccatctc caacggtatt gatatcattc gtattttcga ctgtctgaac gacctgcgca
    29281 acttacagga agcggtaaat gctaccaaca agttcaaggc acaggaaggc agaggtgaag
    29341 cacaggttgc tctggcttac actctgggtg atgcttacac tctggagtac tggaccagca
    29401 tggccaagaa gatcgaagag atgggtgcag attccatctg tatcaaggat atggccggtc
    29461 tgttagtacc ttacaaggct gctgaactga tcaaggcgat gaaggaagtt accaagcttc
    29521 ccatccagtt gcatactcac tatacttccg gtgttgcaag catgacctac ctgaaggcag
    29581 tagaggccgg tgttgatgtg atcgataccg ctatgagccc cttcgctatg ggtacttcac
    29641 agcctgctac cgaggttatg gttgagacct tcaagggaac cccttacgat accggtctgg
    29701 atcagaacct gttagctgag atcgctgact acttcagacc ttacagagat gagtgcctgg
    29761 ctaacggcct gctgaatccc aaggttatgg gtgttgatat caagactctg ctgtatcagg
    29821 tacccggcgg tatgctttcc aacatggtta gccagctgaa ggagcagaat gcagaagaca
    29881 gatactacga tgtgctgaag gagatcccca aggttcgtaa ggatctgggt gagccccctc
    29941 tggttacccc ttcttctcag atcgttggta ctcaggcagt cttcaacgta ctgcttggtg
    30001 agcgttacaa gatggctacc aaggagacca aggcagttct tcgtggtgag tacggtcaga
    30061 ccgttaagcc catgagccag gaagttatcg acaaggttat ccccggtgag gagagaatta
    30121 cctgccgtta cgctgatctg ctcgacaatg agatggataa gctggaaggc gaaatgaagg
    30181 aatggaaaca gcaggatgag gacgttctga gctacgctct gttccctcag gttgctaccg
    30241 acttcttcaa gtatcgtcag gcacagcagg aaaaggttga tatgacccag gctgacacca
    30301 agaacggtgc ataccctgtc taaataattc gtcaataaaa agtcgcacga ctgctcgcag
    30361 tcagtgcggc ttttttatta gacgaacaaa accacatgag caagtagggc gatagcccgg
    30421 attgcgaatg tggttgcgac ttcgagagcg cgaagcgcgg agaagccgtt atttagacat
    30481 gtccccggtc gaaccggggc gatagcccgg attgcnnnnn nnnnnnnnnn nnnnnnnnnn
    30541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30601 nnnnnnnnnn nnnnnaagcc gttatttaga catgtccccg gtcgaaccgg ggcgatagcc
    30661 cggattgcga atgtggttgc ggcttcgaga gcgcgaagcg cggagaagcc gttatttaga
    30721 catgccctta gtcagccccc agtcagcccg atatgcgaat gagtttattt acatattgac
    30781 aaaccacctc ttcacccact aaaataacaa gtgttataga agaacacatt gtttgctgta
    30841 aatacagcgg aatgagaggg attatggctc gtaaagagat gatcacgatt gataaaatcc
    30901 tggatacggc atttgccatg acaagggaag agggatttgc caatgtgaca gccagaaggg
    30961 tagcggcgaa agcagggtgc tctacacagc ctatttttag agtatataag aatatggaag
    31021 aactgtggga tgcggtctat gagagagctg cagcttattt tctggattat tacagcttgt
    31081 atccccgtac cggcaagaca cctttcgcca atctgggcat ggcttacata gcttttgcta
    31141 gagaagagag acacctgttc gagcttctgt ttgtatcgga taaccaggat ggcaaacata
    31201 agaaaaagag catgtatgag atcttgaatg gtgatgccgg aaacgtggtc tacgagatca
    31261 acctggccag agtggcgggc tgcccggatc ccggtgatct gttcatgaaa atgtggattt
    31321 tcattcacgg agctgcctgc atgtctctga ccggagatta tgatctgaca gacttacaga
    31381 caatgcagct attagagcat tcctattacg ctttttacaa tgagacaacg cgaggttaag
    31441 aaccgtggca gaatatgatg tttttacccg aatcaacgaa cattatgcaa aaatgagcaa
    31501 aggccacaaa gccattgcgg attacatatc ggaacattac gatcaggcag tatttatgac
    31561 cgcagcaaag ctgggtgaga cattgggaat cagtgaatct accgtagtgc gttttgcagc
    31621 gggaatcggt tatgacggtt atccgaaatt ccagaaggca ctggaaagct gcgtgcaggg
    31681 aaagctcaac ggcattcaga aaatggatgc caaatacggc agaagctccc agtcggagat
    31741 cctgacctcc gtactcagcg cagatatcga aaagatccag gataccatag acaatctgga
    31801 tccggcggcc tttgaatccg ccgttgccac gattctggag gcggagacca tttatatcat
    31861 gggacttcgc agcaatgagc cactggcagc gtttctgcat ttttacctga atatgatccg
    31921 tggcaaagtc tttttactga ataccaccag tgtcagtgag acttttgaac agatgatcca
    31981 tattggcccg aaggactgct tcatcggtat cagttttccc agatactcca tgcggacgtt
    32041 aaaggccatg gagtttgcca atgaccggaa tgccaaggtg atcgccatta ccgacagcat
    32101 ccattccccc atgaatctgt attcttcctg caatctgctg gccagaagcg acatggtgtc
    32161 ggtggtggat tctctggtgg cacctttaag cgtgatcaat gcgctggtag tggcattgtg
    32221 cctgaaatgt ccggagcagg tgcgtaaaaa tctggagaca ctggaagaga cctggaataa
    32281 ttatcaggta tatctgaatg atgagatcga ctttatagat gatgaaccga tgctggacta
    32341 ttccctgcgg cgcaaggaaa aatgatggac ggacagatcg gcaaaagagg atagaaatga
    32401 gtaaggtgat tgtgatcggt ggcggtgctg ccggcatgat ggctgccatt gcggcagcgc
    32461 aaaaagggca taaggtaata cttctggaaa agaacgaaaa gctggggaaa aaactgttca
    32521 tcactggaaa aggccgttgc aatgtgacaa acgcggcgga catggatgtc ctatttcaga
    32581 atatttgtac caatgaaaag tttctgtaca gtgcttttta cggctttgac aatacacggg
    32641 tatgcgatct gctggagcag gcaggctgcc cgctgaaaac ggagcgggga gaccgggtat
    32701 ttccggtatc cgatcattct tcggacgtga tcgccgccct ccagagagaa cttcgcaaat
    32761 atcaggtgga tgttcatctg cataccgagg tgataaagat cctaacgaag gaaacggatg
    32821 gcaatcctgt tttctgtggc gtggaatgtg cagaccataa gaaaatcgcg gcggatgact
    32881 gcattgtctg tactggaggc tgttcctatg ccagcaccgg atccacgggg gacgggtatc
    32941 gtttcgcgga agagacaggg cataaggtga cggagaggaa accggcattg gtgcctttgg
    33001 agatccggga aaactggtgc agggaactga tgggcctttc cttaaaaaat gtggagatcc
    33061 ggatgcagtg tgggaaaaag acctggtatg aagggttcgg cgagatgctg tttacccatt
    33121 ttggcgtcag cggccccctg atcctgtcag ccagcagctt ttattcgaaa aatctttcga
    33181 aacgtaaagg cggcgatgag gtaaaacttt tcctggatct gaaaccggcg ttgaccccgg
    33241 aacagctgga caaacgtttg cttcgggatt ttgaggaagc aaagaacaaa caatttaaaa
    33301 atgcgctgga ccacctgttc cctgcaaagc tgattcctgt tatgatagag ttgtccgaca
    33361 tttccccgga gaagaaagtg aacgaaatca gccgggaaga gagaaaagct ttcggctcat
    33421 tgatcaagga actacccatg acggtggcgg ggacgagatc ttttgcggaa gcaatcatca
    33481 ctcagggggg aatctcggta aaggatatca atccttccac catggaatcc aaacgtgtac
    33541 ggcatctgta tttcgcaggg gaagtattgg atctggatgc catgaccggg ggctttaatt
    33601 tgcagatcgc ctggtctacg ggacatctgg cgggggacag catacaataa attcgcgcac
    33661 aaaaaagtcc ggagagagat tttacgaatg aaaatcggaa gatttgtgaa aaaataataa
    33721 ttaatagaaa tcaattataa ttaatagaaa taaagaaaag aggactagac tatgagtttt
    33781 aatgtagcaa tcgacggccc ggcaggtgcc ggaaaaagta ccattgcaag agctgtggca
    33841 aagaaaaaag gatttatcta tgtggacacc ggagcaatgt atcgtgccat ggcactgttt
    33901 atgttaagag agggcgtgga acccacggat gcagcggtca tcgccgataa atgcaaggag
    33961 gcggatatca ccattcagta tgtggacggt gagcaggtcg ttttattaaa cggagagaac
    34021 gttaatgctt atctgcgtac cgaagaagtc ggcaatatgg catccaatac cagtgcacaa
    34081 cccgcagtac gcaccaaact ggtagaactt cagcaggcac ttgcagctaa aagcgacgtg
    34141 atcatggacg gccgtgacat cggtacagtg gttcttccga atgctcaggt gaagatctat
    34201 ctgaccgcca gcagcatggt gagagccagg agaagatatg atgaactgac cgccaaggga
    34261 gagacctgtg atctggagaa gatccgtcag gatatcgaag acagagacta tagagatatg
    34321 cacagagaga cctctccgtt aaaacaggct gaggatgcgg tactggtgga tacctccgat
    34381 atgacgatcg agcaggtcat cgaccggatc gtggaactga tcggttaaac aggagatctg
    34441 atatggaagt aagactggca gactgcgcag gcttttgctt cggcgtaaag cgcgccgtag
    34501 atactgtgta tgaacaatta aaaaacggga agactatcta tacctatggc cccatcgtgc
    34561 ataatgagga agtggtccgg gaactggcgg aaaaaggagt aagagttctg gaaagcaaag
    34621 aagaactaaa aaacctgaaa gccggggaga ccattcccac tgtgatcatc cgtgcccatg
    34681 gagtggcaaa agaaatctat gatatcatgg aagcgaatgg tctggagtgc attgatgcca
    34741 cctgcccctt tgtgaaaaag atccatcgta tcgtggaaca aaagagcaca gaagggtacc
    34801 atgtgatcgt cgtgggggat ccgaaacatc cggaagtaga gggtatcgtc ggctggtgtc
    34861 agggcccggt aaccgttctg gaaactcccg aacaggcaga aaattttgtg aaaacagaag
    34921 gtgaaaagct ctgtattgtg tctcagacga catataacta caataaattt caatatatag
    34981 ttgaaatttt tgaaaaaaaa ggttacaatg atagtgttgt gaacactatc tgtaatgcta
    35041 cagaagagcg acagcgatcg gcaaaggcca ttgcagcgga agctgacgtt atgattgtaa
    35101 taggagggaa gcacagttct aatagcagaa aactttacga aatctgccaa agggaatgtg
    35161 tgcacaccta ttttatacag acactggatg acttgcattt agatctacct aaagctgttc
    35221 gactagtggg tattaccgcg ggggcttcca ccccaaataa attaattgag gaggttcaaa
    35281 attatgtcag aattaacttt tgaacaaatg ctggaagaat cattaaagac aatacatgca
    35341 ggagaggtag tcgaaggtac agtcatcgat gtgaaaccgg aagagatcat tctgaacatc
    35401 ggatacaaat ctgacggtat tcttaccaag aacgaataca ccaacgactc cagcgtggat
    35461 cttaccacgg ttgccaaggt tggtgacacc atggaagtca aggttctgaa agtaaatgac
    35521 ggtgagggtc aggttctcct tacctacaag cgtcttgctg cagacagagg caacaagaga
    35581 attgaagaag catttaacaa tcacgaggta cttaaggcaa ccgtatccca ggtactggac
    35641 ggtggtttaa gtgtgatcgt ggacgaagta agaatcttca tccccgcaag cctggtatcc
    35701 gatacttatg agaaggatct gaccaagtat gcaggacagg agattgaatt cgtgatcagc
    35761 gagtacaatc ccaagagaag aagatacatt ggtgaccgca aacagctgat cgtagccaag
    35821 aaggctgagc tacagaagga actctttgag aagatcaaag aaggtgatat cgtagaaggt
    35881 gttgttaaga acgtaactga tttcggcgca tttatcgatc ttggcggcgc agacggactg
    35941 cttcacatct ccgagatgtc ttggggcaga gtcgagcatc ccaagaaggt attcaaggtt
    36001 ggcgataagc tgaaagtcct gatcaaagag atcaacggca ataagattgc actgtccctg
    36061 aagttcgacg acgctaaccc ctggaagaat gcagcagaga agtatgcagt tggcaatgta
    36121 gtaaccggta aggttgcaag aatgaccgac tttggcgctt tcgtagaatt agagcccggt
    36181 gtagatgctc tgcttcatgt atcccagatc gcaaaggagc acattgagaa accttccgat
    36241 gtactgagcg ttggcgatga agttacagct aaggttgtag atttcaatga ggctgatcac
    36301 aagatttccc tgagcgttaa agctctgaca gctccagaac ccgttgaggc agaggaagag
    36361 tccgatgcag atgttgtatc tgtagatatt gacgctgtta tcgctgcaga ggctgagaac
    36421 tctgagaaat aatcaaccgt taggtggtag atattcatga tatatgatta tgagtatttt
    36481 aaaaaggaga tttattctct gactacgatc gatctgaatg cttacaaaga gaagcagatg
    36541 aaacggcgta tcgatactct gatcgccaag cataagatcg taggttatga caagtatgta
    36601 caggcactga agacggataa agtacttttt gaggaatttg taggatacat tactatcaat
    36661 gtttcagaat tctaccgtaa tcctgaacag tggaaatatc tggaagagac cgtgatcccg
    36721 gaactgattc aacgcttcgg caagaacctg aaggtttgga gtgcggcatg ttccacggga
    36781 gatgagcctt actctcttgt aatggctctg tcacggcaca ttccgttaca gcagattcgt
    36841 atctatgcta cagatctgga caaacaggtt atcgcaaagg caaagaccgg cctgtatgga
    36901 gaaaagagca tagaaggcgt gccggaggat ctgaagaaga aatacttcac gaagattggt
    36961 ccgtcttata aaatcgcaga tgagatcaag gcaagagtgg attttcacca gcacaattta
    37021 ctgaaggata cttatcctac cgactgtaat ctgatcgtgt gcagaaatgt attgatttac
    37081 tttacagaag aagcaaagga tgaagtattc cggaagtatt accagtcact ggccaagggt
    37141 ggtatgctgt ttatcggaag taccgaacag atcatgaatt atcgcgaaat cggatttgac
    37201 agaaagagtt ctttctatta tgtgaaaccg atgtagtgag tacagaaaat gacaaaataa
    37261 gaaagaggaa agcagttttg ctttcctctt tttgggcttt ctgtgctata agtgctataa
    37321 gtacatctaa gggacgatgc ctcatttcgt tccggttagt aagtagatgc gatcatggac
    37381 aaaacatgat tggcatactg tgtaaatcct tctctgtttt tcttcatttc atccgtcaac
    37441 tcgaagaata attccttgct cttataatct ctcttgaaga atggcgtgaa cagtatctcc
    37501 cattggggaa gatagcagga gaatttccgg gtgtaacagt tcgctgcgga ggcctggagc
    37561 ctgtgctctt tgtccacgaa accggctaca gatttgtgca atgccacatc ttccgtagga
    37621 agatagtaat agtccttgta gttggaatag aaatatttta attcttcctc gtaaatggga
    37681 acctttaaag tagccgattc tccttctccg cggaaatagc agttggccgc agaagcggtt
    37741 accagtctgg gcagggaagt gggaatctgc agggtgatca acagttcctt gcgttgtgca
    37801 ccgctgacat ctttgtaata gttggcctgt acttttctgg ctttcacaca attctcgttg
    37861 aacagatcat agtaggccag aatcggaaga acttccaaca ttcccttcag gtcgtccgca
    37921 ttatgcaaaa acaatgcttt ttctgtgaat tccgaaggat tttttacata atcatggtat
    37981 agaccaatga gttctccgcc ggagaaaaca tcggtccggg caattccgag atactgctcc
    38041 aaagttttct gcttacagtt cggcagcttt aggaagaatt tataagggga gatccggcgg
    38101 taaatgtcga taccctgaaa tccgtcaaag gagaacggaa gctttaactg cgcacatttc
    38161 tgggtgataa agggaaggtc aaaattattg ccgttgaaat gtaccagata gcgataatct
    38221 cttgcaaaat caaagaaagc agtgagaata tccccttcct gggaatagtc atccgccatc
    38281 cactggatgg tccgccattt gccggcctga tagtaggcac agccaatcat atataaataa
    38341 gaagtacggg aagtgaatcc cgtggtctca atatcgagaa aaaggatttg ttccagaggc
    38401 gccagcttat ccaagggata ggagatcgcg aaatcctcca gagtctcttc tgattttttc
    38461 atgtatgacc tctttctgcc gtgtgggcaa gttaataaac gctttcttcg ttcttcgaac
    38521 tctcgaaagc gtaacaaaca tgcgtaatcc gggctatcgc cctacttact tatgtttgtt
    38581 tattcacctc atagaaaacc tgtgctgcct gcaagcagtc acacagactt tctatgggtg
    38641 aatttatttt accataattt tagaatttgc agtagcattt tgttgataaa agttgataaa
    38701 aatagtacaa ttatcataat gcaatttgca atacaagtaa ctatttagaa aggatgttag
    38761 gaaatggcaa agttgacatt tgtaggcgga atacatccct atgatggaaa agatctgtcc
    38821 aaggacaaac cgatccagga tgtcctgccc aaaggggaaa tggtctatcc gttgtcccag
    38881 cacatcggcg ctccggcgaa gcccatcgtg gcaaaaggag accgggtgct ggcgggacaa
    38941 aagatagcgg aaagtggtgg cttcgtgtcg gcacccatct atgcatctgt gtcgggtacg
    39001 gtaaaaacca ttgagacgag acgtgtggta acaggagatc tgatacagag tatcgtgatc
    39061 gacaatgacg gcctgtatga gagcgaagaa tttcatcctt atgcaccggt ggacaaactg
    39121 cagaaggaag agatcatcga cattgtgaag gaagccggta tcgtaggtat gggtggtgcc
    39181 ggatttccta cgcatgtcaa gctgtctccc aaggatcctg acaaaattga atatgtaatt
    39241 gctaactgtg cggaatgtga gccttatctg acttccgatt accgcagaat gatggaagag
    39301 ccggacaagc tgatcggtgg tctgaagatc atgctgaagc tgtttgacaa tgcccacggt
    39361 attctggccg tggaggacaa taagccggat tgtatctctc tgttaaaaca gatgactaag
    39421 aacgatcctc agatcacggt gaaggcactg aagaccaagt atcctcaggg cgcggagcgt
    39481 cagctgatct atgccacaac gggacggaag atcaattcct ccatgttgcc tgcggacgta
    39541 ggttgtattg tagataacgt agatactgtg gttgccattt acagagcggt gacggaagga
    39601 cgccctctga tggaacgtat catcactgtt accggagatg ctgtggccaa tccccggaat
    39661 tttcgtgtac ctatcggtat gagttatcag gaactgttgg atatagccgg tggatttaag
    39721 caggagccgg agaagatcat ctgcggaggt cccatgatgg gattcggtat gttccagctg
    39781 gatgtaccta ccacaaagac ctctaccgca ttactggcac tgacccagga cgatgtatcc
    39841 gctatggagc ccagtccctg catcaactgc ggacgttgcg tggaagcatg tcccggccgt
    39901 attgttccca gccttttggc aacctatgcg gagcattttg atgaagaggc tttcgtggaa
    39961 cataacggta tggaatgctg tgaatgcggc tgctgtagtt ttgtatgccc ggcaaagcgt
    40021 cctctgacgc aggagatcaa atccatgcgt aagatccagc ttgccaagaa gaaaaagaag
    40081 taggagggaa gaacagtgag cggactttta aaagtatctt caaatcctca tatccggtca
    40141 aaagtaacga ccagcagcat tatgacagcc gtggtgatcg cactgctgcc ggctgccggt
    40201 ttcggaatct ataactttgg ccccagagca ctggcagtca tggctgtgac cattgccagt
    40261 acagtattga cggagttatt attcggctgg ctctggaaga aaaaaatcac gatttccgat
    40321 atgtcagcag tggtaacggg actgctgctg gcgctgaatc tgccggtgag cattccgctg
    40381 tggatggcgg cattgggcgg tgtgtttgcc atccttgtgg taaaaatgct gttcggtggt
    40441 ctgggtcaga actttatgaa cccggcactg gcagccagat gctttctgct gatctctttc
    40501 ccgtcccaga tgacgaattt tgcctgtgat gcctataccg gggctacacc gctggcagca
    40561 ctgaaggcag gagagagtgt caatgtcatg aatatgatcg taggtaagac cgccggtacc
    40621 atcggcgaga cttccgtgat cgcactgctg atcggtgcct gttttctgat cctgatcggt
    40681 gtgatcgacc ttcggatccc cggtacatat attgtgaccg tgatcctgtt tgcgggaatc
    40741 ttcggcgggc acggctttga tcctgcctat ctgtctgcac agttagcagg cggcggcctg
    40801 atcctgggtg cctttttcat ggcaacggat tatgtcacca gaccgattac catcaaggga
    40861 cagtatgtat acggtgcggt actgggactt cttaccggaa tcttccgtat cttcggaccg
    40921 ggagcagagg gagtgtccta tgccatcatt ttaggtaacc ttctggtacc tctgatcgaa
    40981 aaattcacca tgccggtggc ttttggcaga aaggcaggtg cgaaacgtga aaaataagaa
    41041 tgatattcag gcaatgttaa aagaagcggg cattttgttc gcaattactc tgattgctgg
    41101 acttttgctt ggctttgtct atgaactgac caaggagccc atccgtcagc aggaggaaaa
    41161 agcggtgcag gaagcctgtc aggcggtctt tgcggatgcg gagcattttg aagaactgga
    41221 tgcttataca ccttctgcgg atacggcgca gaagctggca gataccggaa tcactatcgg
    41281 taccgtctat gaagcgcagg atgcttccgg aacgcagttg ggctatgtga tccagaccac
    41341 gacctccgaa ggctatggcg gaaatattgt gctgtatgtg ggaatccgtc tggatggtac
    41401 ggtcaatgat atttctattt taagtatttc cgagactccc ggactgggca tgaaagcggg
    41461 agatgtgctg gtgccgcagt tccacaacaa gaacgtgaag agctttgcct acaccaagac
    41521 cggatccact tctgacagtg agatcgatgc catcagcggt gcgaccatca ccacgaaagc
    41581 agtgaccaac gctgtcaacg gaagccttct ggcatttaat gattatgtac agggaggtgc
    41641 tggcaatgaa taaagcaggt gaaagacttt ataacggtat catcaaggaa aatcctacat
    41701 ttgtactgat gctgggaatg tgtccgactc tggcggtgac cacatcggcc atgaacggac
    41761 ttggcatggg cctgaccacg gcagtggtac ttaccatgag taacctgatc atttcgctgc
    41821 ttaggaaagt gatccccagc cgggtgagaa tccctgcatt tatcgtgatc attgcatcct
    41881 tcgtaactgc ggtacagctg ctgttgcagg cgtatctgcc gtcactgaac gatgctctgg
    41941 gtgtgtatat tcctctgatc gttgtaaact gtatcattct gggaagagca gagagctatg
    42001 cgtattccca tccgcccatt ccttccctgt tcgatggact tggcatggga cttggtttct
    42061 ctctggcact gacttgtatc ggtgcagtac gtgagatcct ggcggccgga tccatcttcg
    42121 gatttcggat catgccggat tcctatgtga cgatcaatat tttcggactg gctcccggtg
    42181 cgttcttcgt actggctgca ctgaccgctt tgcagaacta tatcaagaat aagagaaagc
    42241 tggctggcaa ggattacgag aagatccagt ccggctgcgg acatgactgt ctgcactgtg
    42301 gcgagagcgg ctgctcggag cggttttatg acaataccaa taccacggaa gaggctgtga
    42361 aggatgcggc aaaagcggca gcaaaacctc agacggcaga ggatgatctg gagaccatcg
    42421 atttagataa ggaggacaaa taaatggata gtgtaaaaga tatgcttctg ttactgatca
    42481 gttcctccat cgtcagcaat atcgtattaa gccagttcat gggactgtgc cccttcttag
    42541 gcgtgtcccg taagatcaag acggcgggcg gcatgggaac ggcggttatc ttcgttatta
    42601 cgctggccag tgcggtagcg ggtgtgatct acaaatttat cttacagccg ttacacattg
    42661 agtatctgga gacgatagtg ttcattctcg tcattgctgc actggtacag ttcgtggaaa
    42721 tgttcctgaa aaaggcaatg ccttccctgt atcaggcact gggcgtctat ctgcctctga
    42781 ttaccaccaa ctgcgcggtg ctgggtgtag ccattaccaa cgtgcagaag gaatacggta
    42841 ttctgaccgg tatcgtaaat ggctttgcca cagcgtttgg ctttaccatt gccatcgtga
    42901 tcctggcagg actgcgtgag aagatggaat acaatgaagt gccggaatct ttcaagggaa
    42961 tgcccatggt actgctgaca gcaggactga tggccattgc tttctgcgga ttctccggtc
    43021 tgatctagga aggagtaaca tagatgagta taacaggaat tctgattgct gcggcttgtg
    43081 tcggaatcgt cggcatcttt gtgggactgt tccttggcgt ggccggcatt aaattcaaag
    43141 tagaagtaga tgagaaggaa gaggcggtgt tagctgccct tcccggaaat aactgtggtg
    43201 gctgcggctt cgccggatgt tccggtctgg cagctgccat tgcaaaggga gaggctgcgg
    43261 tgaacacctg tcccgtaggc ggagaggaag tcggcaaaaa aatcgccgcc atcatgggtg
    43321 tagaagcaga ggcttccgag cgcaaggtag cttatgtgca ttgtcagggt gactgcgagc
    43381 ggaccacaac ggattatgat tattatggca tcaaggactg tcgtatgatg agctttgtgc
    43441 ctggaggcgg gccgaaatcc tgcaacagtg gctgcctggg attcggaacc tgtgcacagg
    43501 tctgtccctt cgatgccatt catgtagaga acggtgtggc tgtagtagat aaggaaaaat
    43561 gtaaggcctg cggaaagtgc gtggaggtct gccccaagca tctgatcagc ttacggcctt
    43621 attccaatac ggtacaggta gcctgcagtt ctacggacaa gggccctgtt accatgaagg
    43681 cctgcaagac cggctgtgtg ggctgcggta tctgcgtaaa gaattgccag cacgatgctg
    43741 tgaaggtcga gaatttccat gcaatcattg atccggagaa atgtgtgggc tgtggtgcct
    43801 gcatggagaa atgcccgaag aaatgtatcg taaaagcata aagaaagtac aagaggagaa
    43861 aagatatgcg tcttccggac gattatgaag atcacggagg agggctgaca cctacggtga
    43921 tcagtgccat cgttgctgtt acaattttcg tggcagtgat ccttacggtg gtgctgatca
    43981 tgaacagaca gcccacttcc caccaacaga acaatacggc tgcacagacg gagaatgtac
    44041 aacaggcaga aaataatcag acagacaaat atccggacac gcaggacctg atcaccggaa
    44101 gcacattgac tccttcggat ctggatttct gggacatgta cccggagact acggcaacac
    44161 cattgccgtc tgcggatcct tcccaaaagg ataacaaaaa ggaaacagcg gaagcggatc
    44221 cctccaccga tggaaaacat accctggtga ccaaccggga cggcaaggaa gaatgggtgc
    44281 tgatcagccc gtaccttccc aagcacgaat atgatttcac caaactggtg tgccagtccg
    44341 acctgatgaa atattatcag gacggcaaac tgacttccta tgtcggggtg gatatttcca
    44401 aatatcagga ttatgtggat tttttgaaac tgaaaaaagc cggagtggat tttgtgatgt
    44461 tgcgggtagg tgccagaggc tatggcagcg gacagatcgt attggatgat tatttcgtag
    44521 acaatatcaa gagagccaca gatgctggat tacagatcgg ggtgtatttt acctcacagg
    44581 ccatcacgga agaagaggcg gtggaggaat ccaatatcgt tctggagaat atcaaagact
    44641 acaagatcac ctatccggtg gcttttgata tgagttttgt ggacaatgat acgacgagaa
    44701 tcgagactgt cagtcgtgcg gacaagacga agatcaccaa ggctttcctg gataccatag
    44761 aggcagccgg atataagccc ctgatttacg gagacaagga gtggctgatc aaagagattg
    44821 acctgtcaaa attatcggca tatgacgtat ggctttccca gatggaagac gtgccggatt
    44881 atccttatcg ttttaccatg tggcaatatg ccaatgatgt caccatcgac ggcattgcag
    44941 gctatgccaa tatgaatatc agttttattg attattcgga gaaataggct cataattcag
    45001 gattcccggt gcaatgtcga gcgtagaata gatctcggca taacggcggg gagtgatggc
    45061 ttccacatag ttgggggtaa aattactggt gactcctttg ctatgcaaaa tatcgatggc
    45121 cttgttatag gcaatggctg cttcgatatc agtagcgtag cgccccacaa tataattacc
    45181 acggatatga atgcggacag tataaacgta ctgtccgttt tttgctaccg ttttccggac
    45241 accgtgatag atgttgtgga tctgcagatt ttccctgcgg aagtccgtag gatctccgtt
    45301 gacaaaacaa taatccacgc cggtaactcc gtagcttttg atgccatagc gtgaggtcag
    45361 cgtctgctgc attccgtagt cggccacaaa ataatgattt ccccggcaca tgatcttatg
    45421 ggaagaataa tagaacaggt cttccatgtc gaattttaag acatggtggg gggacaaata
    45481 ataatagaag agctgctgtc ccagatagat cggattgccg atgtatagtc cgttgtcccg
    45541 cagattgatc agactgaccc atttttcgaa ggacaaaggg gacaccttct cgtaggactg
    45601 cagtgtcagg gaaggatcgg acagcaggag aagcccctgt tcgtaggccc gatgggcctg
    45661 taaggcatcg gggaaactgc caagactgat atgcttgccg tttttggtca gagaagcacg
    45721 gtaatagaca gttccgtctt ttttttcggc ccggaagact cctttatata attgttcttt
    45781 ttcaatgttt tgctcactca tatttctata gtaacatttt tctccggaga acaacagata
    45841 tatttttcgg acgagctgca taaaatgaag ttagagccaa aatgagaatt ggggaaagga
    45901 acaaagcctg tatgtataaa agagcattaa gcggactact tgcaataaat ctgatatttc
    45961 tgatcggtgt tttaagtctc tgccgcatgg aagaaatctg taggaaattg cagcagacgg
    46021 tgtcggaggt acatatggaa gaaagcctgc tggcggacaa ggaaagcatc atgatgggga
    46081 ttgcttcccg gacttcttcc ggacagagag tagtggattg cagtgaatta cagcgggaga
    46141 gtaagtacca gctggaacag gcggatctgg aagtgctgct tcggatcgtg gaagcggaag
    46201 ccgggtgcga agacgaggat gggaagctgt tggtggccaa tgtgatcctg aaccgtctga
    46261 attccgaaaa atttccggac agcattacgg agatcgtatt ccagcgggaa aacggggtgt
    46321 cacagttttc tcccattgcc gacggcagtt attacaaagt aagtgtcagt gaagagacgg
    46381 tagaagctgt cggacgggct ctggcgggcg aggacatttc caggggagca ttgtattttg
    46441 ccgccagaaa atatgccgac agtgaaagta tgcgctggtt cgatgaaaaa ttgacgctat
    46501 tattccgaca tggcggacat gaattttttt cggataaaaa agcgcttcaa tgattcacct
    46561 tgcaacagtg tttaaagtat gctatacttt tcgaaaatat agatggagat gctagggaga
    46621 tattccagca tacattcata catagacacg gaggagagga aatggaagta atttatcgca
    46681 gcacacgcag caacagcgaa ccggtaaccg catcccaggc aattctgaag ggattatccg
    46741 atgacggagg actgtttgta ccggatcata ttcctgcatt ggacaagagt ctttcagaac
    46801 tgaccggaat gagttatcag caggtagctt atgaagttat gaaattattc ctgacagact
    46861 ttaccgagga agaactgaaa aattgtatca acaacgctta tgattccaag tttgatacgg
    46921 agaagatcgc tcctctggta caggcggatg atgcttatta tctggaattg ttccatggtt
    46981 ccaccattgc gttcaaggat atggcacttt ccattttacc tcacctgatg atcacttccg
    47041 cccgtaagaa taatatcaag aatgagatcg tgatcctgac tgctacttcc ggtgataccg
    47101 gtaaggcagc tctggcaggc tttgccgatg tcaagggtac ccggatcatc gtattttacc
    47161 cgaagaacgg tgtcagcccg attcaggaga agcagatggt gacccagaag ggtgccaata
    47221 cttttgtggt aggcattcac ggtaatttcg atgatgccca gaccggtgtg aagaagattt
    47281 tctccgacaa ggaactggca aaagaaatgg atgagaaggg cttccagttt tcctctgcga
    47341 actccattaa catcggacgt ctggtacctc agatttgtta ttatgtttat gcgtatgcac
    47401 aattattaaa agatggtaag atcacagagg gtgagaagat caatgttgtg gttcccaccg
    47461 gcaacttcgg taatattctg gctgcatttt atgcgaagaa tatgggactg cccatcgata
    47521 agctgatctg tgcttccaat gacaataagg tattgtatga tttcttccgc accggcacct
    47581 atgaccgcaa cagagaattc gtactgacca cttcaccgtc catggatatt ctgatctcca
    47641 gcaatctgga gcgtctgatc taccgtattg caggcaatga tgcagacaag aaccgtgagc
    47701 tgatgagcac tctgtccggc aacggcaaat acgagatcac cgaagagatg aaggcacagc
    47761 tggctgactt ctacggcaac tttgcaagcg aagcggagac cgccgcagag atccgccgtc
    47821 tgtatgaaaa atgcggttat gtcatcgata cccataccgc agtagcttcc gcagtgtacg
    47881 gcaagtatgt ggttgagacc ggagatcaca agaccaccgt gatcgcttcc accgcaagcc
    47941 ccttcaagtt caccagaagc gttatggatg ccattgacat caagtatgat gccatgggtg
    48001 actttgaact ggtggatgaa ctgtctaaga tcgccaaggt gaaagtaccc aatgccatcg
    48061 aagaaatccg taccgcaccg gtgctgcacg atacccagtg tgacgtggac aagatgaaag
    48121 acacggtgaa gagttttctt ggaatgtagt taagaattaa aattagtgag ataccgccca
    48181 agtcacgtca acggttttct gggcggtatt gtttttgtgt aaaaacttta tcctttatct
    48241 tgcgggggtt ctttgctata ataggtaagg ttagcagact gtaacaataa tatgaaaata
    48301 gataccggaa ggagaacaac atgactgcaa aagaaatgct tggaaaagtg gatcataccc
    48361 agttgaaagc ctttgctacc tgggaggaca tccagaaatt atgcgatgag gcgattcagt
    48421 atcataccgc cagcgtatgt gtgccccctt gctatatcaa gagaatccat gacacttacg
    48481 gagacaaaat caatatctgt accgtagtag gattcccctt aggatacagt gtgacggaag
    48541 ccaaggtggc cgaggtgcgc aaggcactgg aggatggttg caacgagatc gatatggtag
    48601 tgaatatcag cgatgtgaag aacgggttgc ttgacaaggt ggaggaagag atccgtacac
    48661 tgaagaaaga gtgcggacat catatcctca aggtgatcat tgagacctgt tatctgaccg
    48721 aggaagagaa gattgccatg tgtaaggcag tgaccaatgc cggagcggac tttatcaaga
    48781 cttccaccgg cttcggaact ggcggtgcca caaaggagga tgtggaactg ttcaagaaat
    48841 acattggccc taacgtaaag atcaaagcgg caggcggcgt atccagcatg gaagatctgg
    48901 aaatgttctt aggacttggc tgtgaaagaa tcggtacttc cagagctgta ggactctgca
    48961 agggtgaact tacggaaggg tattgattaa ctgatatata gatgatatat agaatggaga
    49021 taacatggaa aacaaagtga ttcaggacag acttgcactt cttcggaaga agatgcagga
    49081 agagggcatt gatttctata tgatgcccac agcggatttt cataattccg aatatgtaaa
    49141 cgattatttt aaagtaagag aatatttctg caattttacc ggttccaacg gcacactggt
    49201 agtctggaaa gacggcgcgg gactgtggac tgacggcaga tatttcatcc aggcagaagc
    49261 agaattggag ggaacgaccg tagaactgtt ccgtatgtta caggagggag ttcccaccat
    49321 cgaggaactc ctggagcaga acatgcagca gggacagact ttaggatttg atggcagagt
    49381 tatcgctgcc atggacggca aaaaatacga aaaggtactg gaaaagaagc agatccgcat
    49441 cgcttatgag aaggatctgg cagagagcat ctggacagac agaccggatt ttcctgccgg
    49501 aaaagtaacc gtactggatg agaaaatcca cggtaagagc gtagaggaaa aactgacaga
    49561 gaccagagaa aagatggcga aggacggtgc caatgcactg cttctgacca aactggatga
    49621 tctgatgtgg ctcttcaata tccgcggttg tgatgtggaa tgcaatccgg tagcaatgtc
    49681 ctatgcctat attacagaag agtccgcaac tctgtttatg cagcagaaag cactggatgc
    49741 acaggtgacg gaatacctgg cagctcatcg tatcgaggta aaggaatacg acaccgtcgt
    49801 agatttcctg aaggcactcc ctgcgggcaa tgcgatcctc gtggacaaca gatattgcag
    49861 ctatacttta tacaagacat tgcagaagaa tcagaagtta gtagagggca agaatcccac
    49921 cgaactgtta aaggccatta agaatccggt agagcttgcg agaatgcagg agatcttttt
    49981 aaaggacagc gtggccgtaa cgaaatttat ctactggctg aagacccatg tgggcaaaga
    50041 gaagatcact gaggtgaccg cagcggacta tctggaacag aagagaagag agatcccgga
    50101 attcttagat ctgtccttcc cgaccatcgc aggttacaag tccaatgcag ccatgatgca
    50161 ttacgaagca accccggaca actgtgcgac tctggagccg gaaggaatgc ttctggtaga
    50221 ttccggcgga cagtatctgg gaggtaccac cgatgtgacc agaaccatcg ttttaggtcc
    50281 catttccgaa gagatcaaaa agcattacac catggtagca gcggcagtga tgcagctgac
    50341 ccatgcacac tggctctacg gctgtaccgg acggaatctg gacattctgg caagacagcc
    50401 tatctgggat atggacattg attaccagtg cggtaccgga cacggcgtag gttatattct
    50461 gaatgtgcac gaaggtcccc agaacatgcg ctggagattc accggaggta tggtggaggc
    50521 agtgtttgag gatggtatgg atatcaccaa tgagcccggc atctatattc agggcagcca
    50581 cggtatccgc atcgagaatg ttatggtggc gaaaaatgat gtgaagaacg agtatggtca
    50641 gttcatgcat ttcgagaccc tgacatgggt acccatcgac agagaagcca ttgatgaaaa
    50701 gtatctgaac gatacccaga aaaagtatct gcacgagtat cagaagactg tctacgagaa
    50761 gatttctccc tatctgaatg aggaagaaaa agagtggctg gccaaggaaa ccagggtaaa
    50821 gtaaagagaa tataaaatca gtataaaatt tttagcggtt tcttgacttt tttactgggg
    50881 tttgagataa tacagacgat gtggcagaag ataaagacac acataaattt aaaggaaaga
    50941 ggttaatgta agatggcaga gatcaattta agcaagtacg gcatcaccgg aaccacagaa
    51001 atcgttcaca atccttctta tgaagaatta ttcaaagaag agatgaagcc tgaactgaaa
    51061 ggctacgaga aaggtcagga gaccgaactc ggtgcggtaa atgttatgac cggtatatat
    51121 acaggacgtt ctcccaaaga taagttcatc gttgaagatg agaactccaa gaacactgta
    51181 tggtggactt ctgatgagta caagaacgac aaccatcgta tgtctcagga aacatgggct
    51241 gctgtaaagg atatcgctaa gaaggaactg tccaacaaga gactgttcgt tgtagatgca
    51301 ttctgcggtg ctaataagga cacccgcatg gctatccgtt tcatcgttga ggtagcttgg
    51361 caggctcatt tcgtaaccaa catgttcatc aaacccaccg aggaagagct gaagaacttc
    51421 gagcccgatt tcgtagttta caacgcatcc aaggctaagg ttgagaatta caaggagctg
    51481 ggtctgaact ccgagacttg tgtagcattt aatatcacta gccgcgagca ggttattgtt
    51541 aacacatggt acggcggaga gatgaagaag ggtatgttct ccatgatgaa ctactacctg
    51601 cctctgaagg gcattgcttc catgcactgc tccgcaaaca ccgatatgaa cggtgagaac
    51661 accgcaatct tcttcggtct gtccggaacc ggtaagacaa ccctgtctac cgatcctaag
    51721 cgtctgctga tcggtgatga cgagcacggc tgggatgaca acggcgtatt caacttcgaa
    51781 ggtggatgct acgctaaggt tatcaacctg gacaaggatt ccgagcccga catctacaac
    51841 gcaatcaaga gagatgcgct gcttgagaac gtaaccgttg atgctgacgg caagattgat
    51901 ttcaacgata agagcgttac cgagaatact cgtgtatctt atcctatcaa ccatattaag
    51961 aatatcgtac gtcctatttc ttccgcaccc gcagctaaga acgtaatctt cctgtctgct
    52021 gatgcattcg gtgtactgcc tcctgtatct attctgactc cggagcagac ccagtactac
    52081 ttcctgtccg gtttcacagc taagttagct ggtaccgagc gtggtattac cgagcctacc
    52141 cctaccttct ctgcttgctt cggtcaggca ttcctggagc tgcatcctac caagtatgca
    52201 gaagagctgg ttaagaagat ggagaagaac ggtgctaagg catacctggt taacaccgga
    52261 tggaacggaa ccggcaagcg tatctccatt aaggataccc gtggtatcat cgatgctatt
    52321 ctggatggcg ctatcaagac cgctcctacc aagaagatcc cttacttcga tttcgaagtt
    52381 cctaccgagc tgactggtgt tgacaccggt attctggatc ccagagatac ctacgctgat
    52441 ccttcccagt gggaagagaa ggcaaaggat ctggctcaga gattcatcaa gaacttcgct
    52501 aaatacgaag gaaatgatgc tggtaaggct ctggttgctg ctggtcctca gctgtaatct
    52561 tactttacag acaaatgcaa atatggaagg ctgtctcttc ggagacagcc ttttttgcag
    52621 ataataatat tggtaaaaat tacagttgta aaatggatct taactcgcta taatatactt
    52681 aacctgagat gtacgataac tcttaccagt tttgaaccta catttacttt aaacgggatt
    52741 cctatattgc caagtggctg tcaagccctc actccgcaag gaggattgga agggaaggca
    52801 aaaggttggt tgcggttacc cacctagcat tgctaggagt caaaaggtaa gaggcggcat
    52861 tttggggata acaatacaaa agagagagca gtcctttggg actgctcttt taaagtcata
    52921 aaacagattc tgaataggcg ggaaatgatt tgaagaaaaa cagactgctt gagaattatt
    52981 ataaactttc cagagaacag agaatacagt ggaagaaata cctgtgtgtg ttaggtacgg
    53041 catttcttct ttttttactt ctgataaatt tgttacatag ctgcggtcgg gaggagccgg
    53101 gggcaccgga gacagaagag gccctgcctc agcatattcc agtagtacgg gaattgaaaa
    53161 acgtctggat cacggaggcg gaagctgggc agattacttt attctgtgac ggagtaagag
    53221 agacctatga tctggataca gaggcgaatg aggcaggcag gctgccgaca ccggaccaga
    53281 tgcgggaaca gctggcagat gtggaactta cggatgacct cgtgagtgcc gttatcctga
    53341 aaacagagaa gttcacaggc cgggtgctgt cggcagatga aagcggtatc gagatcgagg
    53401 gcaggggccg gatcccgttg gctgaggatt acaaagggta tcgcctgtac cgggagctta
    53461 ccatgtgtac cactgaagat ctgcgattcg gctatatgga tgcggacttt attcgtgaaa
    53521 atgacgagat ctgcggtatc ctgctggcca gagaagataa catggacaaa atccgggtat
    53581 tgatcaaaac ttcggatttt tcggatgttt tgcaccagac ggtgacactg acggcggaaa
    53641 gtgattttct actacagtac ggaaccggtg aggaaatgca ggaagaattg ttttccaggg
    53701 gagacgaggt gaccattgac acagacagcg attatttcgt gggagaacgg atccgtatag
    53761 taccgacagt gctgaccggc cgggtacggc tgatcaatgt gaatcgaagt cagggaacac
    53821 cttcttacag agggcatata gagctgctgc ggaccgcaga cggaattgca gtggtaaatg
    53881 agcttccgct ggaagaatat ctctacagtg tagtgccaag tgagatgccg gcatcctacc
    53941 cgctggaagc cctgaaagca caggctatct gtgccagaac ctatgcctac ggacatatgc
    54001 tgcgggcggg ctatccccgg tacggcgccc atgtggacga cagcacttcc tatcaggtct
    54061 acaataatat cacagaagct gacagtgcta ccacggcggt gaaggagacc tacggacaga
    54121 tgatcctgac agatgaagga acggtggcga acacttatta ttactctacc tcctgtggcg
    54181 tgggaactac ggcaaatgta tggaagacag cggaggcgga ggcacttgat tatctgaaga
    54241 gcagccggct gaatccggag agcctgatgc agacggacgg tggggtaatc gcggcagaca
    54301 gcaatgaggt aaacagagat gcaggtccag aggatctgag ggaagaagag gctttccggg
    54361 attttatcac ggagacacat gcggaagatt atgaagcgca ggaaggctgg taccgttgga
    54421 catatacggt aaaagagatt gatgtggatc ggatagtgga gacgctgaag aaacgttatg
    54481 aagccaacgg aaagttaata ttgactctga aggacgatga ctatgtcagt caaaatataa
    54541 aagctttttc aaaagtaaac gatatctccg ttgtaaaacg tggccccggt ggtgtggcag
    54601 acgaaatggt cattgtcacg gacaaaggaa cctttaaggt gatctcggaa tataatatca
    54661 gagcagtact ttgtgacggg gttacgaagg tggtaaggca ggatggtagt gaggtggcca
    54721 tgccgaatct ccttcccagt gcattttttg tgattgaacc tagccatgat aagaaaaatg
    54781 tggtaggata taacattatc ggcggtggat tcggccatgg cgtgggaatg agccagaacg
    54841 gtgccaaaaa tatggcacta caggggcttg gcgcggaaca gatccttaac tttttctatg
    54901 aaggatgcga gatatgctca ggacaataat cagcatctgg cgtgattttt ccagccagat
    54961 gaaaaaacag aatatcagcg cttatgcatc gagtacagcc tttttcctgt ttttgtcggt
    55021 gattccgatg ctgatggtag tctgtgcggt gattccttat accccggtga cggagcagaa
    55081 tctggtcacg gcgctgacgg atgtgacgcc ggatattgca gatgccatgg tggagagtct
    55141 ggtcgttgat gtgtatgaga gttctgtggg aattctgccg gtggctctga tcgctatggt
    55201 ctggtcggcg gcgaagggtg tcatggcact gatgcgggga ttaaatgcgg tcaatggtgt
    55261 ggacgagaag cgtaattact tcgtgatccg ttttattgca agcttttata cgttgatcat
    55321 gcttgtagtg ttgattttgt cactgttttt catggtattt ggtaatcagc tggtggatat
    55381 tgcattacat aggattccgc agctgcagat gtttgtgagt ctcctgatga atttccgctt
    55441 tctgttcgta tgggcggtgc tgattctttt gtttggcctg atttatacat atatcccgga
    55501 cacaaagctg aaattcacgg aacaggttcc gggagcctgc tttgctgcgg tagtatggag
    55561 tatattttcc tggggatttt ccatgtatgt gtcctatgga aatgggtata gtatttacgg
    55621 aagtttgacc attattgtga tcattatgtt gtggatgtat ttctgcatgt atatcattct
    55681 gatcggtgct tatctgaacc gttatttccg tccggtgaac cgggtactgg tgaatcatgc
    55741 cagcagtaag agcgaactgg caagagagaa ccgtagacgc atccgggaga tcaagaagat
    55801 ccagcgcaga aaaaaacatt atacggagat cgacagggat cctaaggggc aggatgaaaa
    55861 tctgacagac aggaattgaa ctgcggacgc aaatgtagta cactgatacg gagagggcga
    55921 gggacctctc ctgttatgat tatagtaaaa gagtatccgg aagacggatg cggaaatgag
    55981 gataaatatg gaaaagaaaa acacctggga gacctattct tccaaacagt taaaagagtt
    56041 ggagaaaata aacgcacagt atcgtgattt cttagacaac ggtaagaccg agagagagtg
    56101 cattgacacc attgtcaata ctattgagaa gtccggttac cgtgaactgg agagtctgat
    56161 caaagagggc gctgctctta aaaccggaga taaggtatac agcgtatgga tgaacaaatc
    56221 catcgccatg ttccagatcg gacgtaagcc catgacagat ggtatgaaca tcctcggagc
    56281 ccatatcgac agccccagac tggatgtaaa gcagaatccc ctgtatgagg atggtggatt
    56341 cgcttatctg gacacccatt attacggtgg cgtgaagaag taccagtggg tgaccattcc
    56401 actggcaatc cacggagtca tcgtgaaaaa ggacggcacc tgtgtagaaa tcaatatcgg
    56461 tgagaacgag gatgatcctg tattcttcgt atccgatctg ttgatccatc tggcacagga
    56521 gcagctggag aaaaaggcag caaaggttat cgagggtgaa gctctggata tcatcgtagg
    56581 caacaagcct ctgctgatcg ataagaaaga taagaagaag gacgagaaag aagccggaaa
    56641 agaagcagta aaggcaggtg ttctggatat tctgaaagag acctacgata ttcatgaaga
    56701 ggatttcgtt tccgcagagc tggagatcgt tcctgcagga aaagcaagag aagcaggctt
    56761 tgaccgcagc atgacactgg cttacggtca ggatgacagg gtctgtgctt acacttccat
    56821 gcaggcgatg ctggacagcg atgtgaccga ccgtaccatg tgctgtattc tggtagataa
    56881 ggaagaaatc ggctccgtag gtgctaccgg catgcagtct catttctttg aaaatgccgt
    56941 ggcagaggtg atgaatctgg caggcgaata cagcgaactg aacgtgagaa gatgcctggc
    57001 acatagctgt atgctgtctt ccgatgtcag cagtgctttt gatcctacct atgcgtccag
    57061 cttcgataaa aagaacgtgg catacttagg cagcggtatg gtcatcaaca aattcaccgg
    57121 agccagaggt aagtccggtt ctaacgatgc caatgcggag tatctggcac atttgcgcca
    57181 tatcttcgac aaagcagagg tgaacttcca gactgcagag cttggtaagg tcgatttggg
    57241 cggcggtgga accatcgcat atattctggc actgtatggc atgaatgtca tcgacagtgg
    57301 tgtggcagtg ctgaacatgc atgcaccgtg ggaagctacc agcaaggccg atgtatatga
    57361 ggcatatcgc ggatataaag catttgtgga aggagcgagc ctgtaatggc agagacgacc
    57421 gaactgaaaa aatccgattt ttactttgat cttccccagg agctgatcgc acaggacccg
    57481 ctggaggatc gttcttcctc cagactgctg gtactggata aaaatacggg agagatctct
    57541 catcacattt ttcgggatat tatcgactat ctgcatcccg gagactgtct ggtgttaaac
    57601 aacaccaagg tgattccagc gagacttttg ggagaacggg aaggtaccgg cggacatgtg
    57661 gaagtccttc tattaaaacg aaaagaagcg gatgtctggg agactctggt aaagcctggc
    57721 aagaaatgca agcccggtca gcgcctgacc ttcggggatg gactattaaa ggcagaagtc
    57781 ctggagactg tggaagaagg taatcgtctg atacgttttg aatatgaggg tatttttgaa
    57841 gaagtgctgg acaagctggg agagatgccc ctgcctcctt acataaccca taagctgaag
    57901 gataagaatc gttatcagac cgtttatgcc aaatatgaag gctccgcagc agcacctacg
    57961 gcgggattgc attttacgca ggaattgttg cagcagattg cggacaaagg cattaagatc
    58021 gcttatgtga cattgcatgt gggactggga actttccgac cggtaaagga agagaacgta
    58081 ctggagcatc atatgcattc tgagtattat caggtgacgg aagaagcggc gaacaccatc
    58141 aatgagacaa agaaaaatgg tgggcgtgtg atctgcgtgg gtaccaccag ttgccgtacc
    58201 gtggagagcg cagcggacga gcagggcatg gtacagcccg gctgtgacaa taccgagata
    58261 ttcatttatc ccggatatcg tttcaaggtg caggatgcac tgatcacgaa tttccatctg
    58321 ccagagtcca cactggtaat gttggtatcg gcacttgcgg gaagagaaca tatcctgcac
    58381 gcctacgaag aagcaatccg tgaaaaatac agatttttca gctttggaga cgctatgttc
    58441 atcgctgact taaaaggaga aaaatagtgg aagaaagaag acgaagcaag cgtacagaaa
    58501 tgagttccac cctgatggta aagcgcctgg accagggaga catgcaggaa gtcagcatag
    58561 acatcgtaga tgtatctccc aacggagtgg gctttttgtg caaggaagtg ttaagtatcg
    58621 gtgcagtgta tgaatcgaaa ctgaggattt ggacgcatga agtgctgcat gcgttcttac
    58681 agatcgtcag gatcgaaatg aagggagatt cttatacgta cggggctgtc ttcatcggaa
    58741 tgtcggagag tgatgcggca agaatccgcg tataccagac gattaacgac gatgaaaaac
    58801 aataagaaaa aaatagcagt cagtgttgtg gctgctattt tacttgtact gttatatctg
    58861 ttgattttcg ctttttctgg tcaggacggt gaggaatcag gctcactgag ttctatgatc
    58921 tcggaaaaat gtgcggagct tctgaatgct atttccagga aacactggac acagaatgtg
    58981 atcaacagta tggcagctta ttttgaacat ccaatccgta aactggctca tttctccgag
    59041 tatgcatgca tgggagtgct gctgtacggt gtgtggagac cctggaagga gaggaaccgg
    59101 aagctgtatc tgctgatcgt actctgggtg tttgtctccg ccggagcgga cgagttccat
    59161 cagttatttg tcccaggacg ctatgggtgt tttgcggatg tggtgctgga tacctgtggc
    59221 ggtgcgtttg gactgctcgt atgtgtctat gtggagaaaa tagtgcggcg aaggaaacag
    59281 aagcggaaag ataaggaaaa agagatcact ttgtaggtga tctctttttg tgtagtgcga
    59341 tgagccaggt acagggtttc tacttcctgt tgtgcagact
//
LOCUS       DAWEJW010000004        52627 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_313_length_52627_cov_63.719299, whole genome shotgun sequence.
ACCESSION   DAWEJW010000004 DAWEJW010000000
VERSION     DAWEJW010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 52627)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 52627)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..52627
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_313_length_52627_cov_63.719299"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2125..2224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11442..11541
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14692..14791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18196..18295
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    42175..42274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    50554..50653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        12298 a        13904 c        11446 g        14379 t
ORIGIN      
        1 tgtccgttcc tgctctttac ccattttttc atgctgaagg gtttttttgt tttcttcatt
       61 gtctctgcat tcatcgaaat acctccatac ccttctctct tagataagag ggaaccggat
      121 aaatggttcc ctctcacttc agaagattaa attagttacc tattaattac tggatctcta
      181 caccacagct ctcctgtgcg gaagtccact cttcgttcca ctcgtcgatg atctcatcat
      241 aggtcttgga accatctgct gcacactcta ccagctcctg catcttggtc tcaccgttgt
      301 tgttgatgtt aagaccactt tcagcgttca gctcgtttaa cagatcctct tcgccttcga
      361 tcgcaggctc atccactaca aagtcgatac ccttgaatgc tgcgtagaaa tccggtaatc
      421 tggtatcctt gatagcaacg ggaagtccgc cgcagtcata ggagaatccg gattcttctg
      481 ccatgaactt aacgaagcac agagctgctg cctgattgtc ttcggtggta ttagcattga
      541 taccaaagct gtagtcaggg cctacagatg ctgcctgctt accgctggtc agggtaatag
      601 ggaaaggcat ataagcgatg tcgtcagcgt tgtcaccggc ttccttcatc tgaggaactg
      661 cccaggagcc aagaaccatg caagcgatct gaccattgtt gatcataccc ttacaacctt
      721 cccagtccgt ggtggagtag tcttcctcgg taaggccttc agcaactgca tcgtacatta
      781 ctttgtatac gttgtaagcg cctgttccgt cgccgggatc agcaaaagga ttggctgtgt
      841 gcagtaactt ctggttcata taagtgctgt ctccggtagc actgccgctg atagcgggat
      901 cccactgggc gcccattgcc cagcctgctg cgtagttggt gtacaaagga acgatagagc
      961 tgtcatactc tttgatctgc tttaatgctg cgatgaaatc tgcgggagtc ttaggtatct
     1021 ctgtgattcc tgctgcttcg aatactgcct tgttgtatac gatgccgccg ttaacgcctg
     1081 cggtgtaagg aataccatat accacgcctt cgtacatctg gttgtagtag ttcagctgtc
     1141 cgtccatctc ggtagtagtt ccaagaggaa ggaagtatgt gcttaagtca gccttgtcaa
     1201 ctgcggggat aaacataaca tcgccccagt caccaccctg cagacgaagc agcgtatcac
     1261 ttgcatagtc ggtgatacct tcaatatcaa cagtgatgtt gggatacatc tcgttgaatg
     1321 catcaatgta agtctggaaa ggaactgctg cgccgtcctc taacaggtcg gtacggttag
     1381 tcaggacctt aatggtcgtg gtaatgtcgg tgtaatcctc acccaaagta agctctgcgt
     1441 aattcaggct ggtaacctct gcaggtgctg cagcctcgct ggaagcttct gcagttgcgg
     1501 gagtggtgct tgctacagaa ctctcagcgg cgccgccgtt gtctgtgctt ccacatgctg
     1561 ccatagacag tgccatgact gatgctgtgg aaagagctaa aaactttttc atcttcataa
     1621 gaaatcctcc tagttttccg aaggaaaatc cgagtcctct gacgaggaga ggatttagcc
     1681 tccttgtttt ttgctacatt tgatagcgtt tactgttttg taacttaatt taattataca
     1741 taatcatatt aattaatcat caactattct tgtttttaat ctcttattct tgctattttc
     1801 gttattatct tctattttca caagttctat gcctatgttt tgtacatatt atacaattaa
     1861 agtaattaat ttgaattaaa ttagctgatt taattaacta attttgtttt ctttgcatcc
     1921 tttgtcattg aaacagctta aataatggct tataatgtat ttaagcttaa ttattttttt
     1981 caaaaagcat gtttctagtg tgaaaataaa tttttcacat gaagttttgt tcctgcacaa
     2041 gctgctaaag cagccgaagt ttgaagattg cggaaaggca aggtttttgt atgaacattt
     2101 ccaccacttt tctcaatgca ttgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnngcggaa aggcaaggtt tttgtatgaa catttccacc acttttctca atgcattgag
     2281 gcacacttcc tccgatgtgg aggggcagcg ttccccggaa atcctgctgg ggcagatcta
     2341 taaagtatcc ctggaagaca agagcttttt actgcccctg gccggtggag aattccggct
     2401 ctcggatcct ttctattatg aatggaaccc ctttccctca tatctgctgc tttacacctt
     2461 agagggcagt gccgtcctta ctacccgggg cagcgaggtg gtcctggatc acgagcatct
     2521 gttgttctta gattgcagcc aggggttctc tctccggctg ttgcagggaa agtggcgttt
     2581 tcagatgttt ttcttaagtg gagcggagct ttcttcttat tatagcatat ttcaggctgc
     2641 ttttacctgt tgttacccga ttcccatcgg ctcctgcgtt tcacagcgga tgcagcagct
     2701 gaccaaattt tcattcaccc cgactcctgc agagcagata cagcttcacc gagaactgac
     2761 cgacatgttc gcagaactga tgctgtccgc cctgtccgtc tcccgggatg tgaagcccat
     2821 tcctgcttat ctgctgtcca tgaaagcgga ttttgaagaa aactatgctg aaaatcactc
     2881 tttggaatac tttgagcaga ctttgggcat cagccgctac cgtctgtgtc gggaattctc
     2941 cgctacattc catacttccc cgctgcaata tcttcagggg aaaaaaatag aagcagcaaa
     3001 atcactgctc ctatctacgg ataatccgat tcacgaggtg ggggctatgg taggcatcga
     3061 caataccaat catttcatcc acctgttcaa aaagcacacc ggtgtcacac ccttcgtcta
     3121 caggcagaat gcaccgcagt ctatacgtga gacacactgt ccttcagtac cagtcggcct
     3181 tccaccacgg taactccgga cttataatta tcactggtga tcttcttgat catattatga
     3241 atggctcttc tcgccatctc tcccatatct acttcatacg tcgtgatatg gatgtcactg
     3301 agccccggat acaggtagtt atcaaagccg accacagaga tgtcctccgg cacacgatat
     3361 ccgttctctc tcagtttctt gatcagaaag ctcgcagtca gatcgcagtt gcatacgaat
     3421 gcggtaggca tatccttggg aagctgcaac atattcaccg tatcgatctt acctgtctca
     3481 atgtgccgat cgtccacgac ccattccttt ttctgctcca gtcccagctc caaaagagcc
     3541 ttctgataac caaaataccg gtccgtaata ctctcggtag caagcagggt tcccacataa
     3601 gcaattttgt gatgtcccat ccgatacaga tagtaggtca gttcataggt tccgtaataa
     3661 ctgttggaaa taacggaatc gctggcttcc cttccgttat aatagtccag atagatcacc
     3721 gggatcccgg cttctgtatt cagatgctgc aggtaagtat ccatcatctt accgataacg
     3781 ataatgccat ccaccttgcg ttcctgtacc agcttcggca ttctgccgtt ctcttcctct
     3841 gacattccga tgacctccag catggtaaag cattcctttg ccgtggctct ggtagctacc
     3901 gcctggtaca tctgccagta aaatgattca tatttatcaa ggaatctctc agagatcaga
     3961 ataccgatat tgtagctctt ctggttctga ctttttcttg ccgctgacgg ctgctgataa
     4021 cccagttcct ccgcaatgct gcggatcttc tcccggactt cctcgctgac gcctttttgt
     4081 ccggacagtg ccttggacac tgttactgtg ctgactccaa ctctctctgc gatatctgct
     4141 aattttactg cttttgccat aattttgtac tccttatcgc tatttttcta cacatttccc
     4201 gataaatcgt caattatact tccatattag atttctaaaa atcgtttgtc aaggcatgtc
     4261 actgccttaa ttttacacct cggtcagatg gatcagctta ctgcggtagt ctccccacag
     4321 aggtgtcacc agcagtcctg cctgcatcag cacgtcgccg ccaaagattt ccccggtctc
     4381 ctcgatgcga tactgtttct ccggtgccag gcctttcaga cgtactctcc tgctgtggaa
     4441 gttcggtcgg gaaagtacct gtacataggt taccaacgct tctttctgat ccttggatgt
     4501 cacctgatag cagtcataat taccgttttc caaaaatgat gcaatccggg aataatctcc
     4561 tctgcggatc aggtcgttgt atttgtgata catggcggtc tgtgctggaa tctgctgtct
     4621 gtccttctcc gggatcttcg taatgtccag ttcatagccg aaggtgcctg ccagtgccac
     4681 atgacctctg gtctcaaaag gagtcactct tcccaccgta tggttggggc agtccgatac
     4741 atgggcgccc atggtggaca gcggatacaa taacatcgtg ccctcctgaa ttttcaatcg
     4801 ctcaatggca tccatgtcat cggagcacca gatctgcggg ctgtaatata acatacccgg
     4861 atcaaatctc gctccgccgc cggagcagtt ctccagaagc agatgcggga attcctgcag
     4921 cagtctgtcc tgcagttcat acacacccag cacatatctg tgatagagct ctccctgacg
     4981 gtctgcgggc agttccacgc ttcccagatc cgacagctgc ctgttcatgt cccatttcac
     5041 atattcaata ttggcactgt gcaacactgc cgccacgctt tcaaaggcat gatctctgac
     5101 ttcctttcgg gacagatcca gcacatactg gttcctgcat aagctgcctg cccgtcccgg
     5161 aatggcgatg gcccagtcgg gatgctctct gtacagctcc gaatccgggg agatcatctc
     5221 cggctcaaac cagataccaa acttcattcc cagtgcattg acctgctcca ccagagattt
     5281 taagcccccc ggcagtttct gctcattgac cttccagtca cctaagctgg tactgtcatc
     5341 gttacggtgt ccgaaccagc cgtcatccat gaccagcatc tcaatgccga gctttgatgc
     5401 ctgttctgcg atcttcagta gtttttccgt gttaaaatca aaataggtag cttcccagtt
     5461 attgatcagg attggtctct ttttatcacg gtactcgccg cggatcagat gattgcggta
     5521 catatcgtgg taatggcggg tcatgcctcc cagaccttcc gcagaatagg tcataacgac
     5581 ttccggggcc gcaaagctct cgccctgctc cagtttccag acaaagttct ccggatgaat
     5641 gcccatagtc atacggatgg aatcgaactg ggaaagctcc gcctgcgcca ggaaattgcc
     5701 ggagtataca aagtgcatac cgtagacttc accggcatcc tgcgtggcat ccgcagaaag
     5761 cagtgcgata aagggatgct cctgatggga agattctccc cgcacggagc caacactctg
     5821 tttgccgtgc atcagacttc ttctctccat ctgacgctct ctcgcccagg atccgtgcag
     5881 cgtaagaatg tcatactttt catccgtgca gtccagatcc aggcaggcag acataacttt
     5941 tgtcacataa ataggctgcc ctgccgtatt tttcaggatc acactgcggg tgatcacatc
     6001 ttcctcttca aatgtagtat agatcaggtc tgcagccaga ccgatgaccg gatccgccag
     6061 atgtaggatc agggtatcgc agtcctcatc ccccggaaat gtggaaggaa gtcccggcag
     6121 tgccggcttc tcctttacga tctcatgaga gacatactgt aactgcactc ctacatgtcc
     6181 ctgcgctgtc cgcacggaaa tggcgctttc tctgtaatct cccagaccgt tccctggata
     6241 ctccacgggg aaggaatcca gaaatgacac acgctctctg ttattctgtg acggaacgaa
     6301 gggtgcttct cccgtacgca gcaaatatac cggtgccggt gtattctcgg tatactgcaa
     6361 tttctgaccg taatatacgt gtcccacaaa attctcctga tctacaattg ccagaagata
     6421 gctggtgtgt tccgtgtcca accagaaagt tctgtcctgc tcgatgtatc tgatcgccat
     6481 aatctgcctc tttctttatc aatgtttatt cttaaaaaac cgtttttgca aaatcatttt
     6541 tccaaatttt ccaaataata tccatgacag tgtatttgca tctgtcaatg tcacagatat
     6601 aaaaaagcaa acggtgcttc cggttctgtc ttcaaaaact gcatcagcat cagtccacag
     6661 cgcatggaca cccgttcttc ccgcaggata cgaagtactt cctgtctgtc tgccaggatc
     6721 acctgaatat cctcggtatt ttccatctgc tgtcccacat tctctgtcac ggttccgaag
     6781 atcatgctgc cgctctcgtc tgtcagcccc tgtgccagga aaaatcctct tcggaaagcc
     6841 ggttctccgc cttcgtagac ttccagtttc cagccggtct cctcgatcat ttcccgacag
     6901 gcggcctccg aagcggtctc ctccggctcg atcagtcccg ccggcaattc atacagataa
     6961 tcattcacgg gataccggta ctgacgcacc agcaccattt tctctccgtc ctctgtcact
     7021 gcgtagaccg ccatgccctc ggggcgcata ctgtgggtct tgtgtttgat attgttttca
     7081 ccatttcggg acgcaaaata ataatgaaac ggcgttccgt cccgtcccag ggcatcaatg
     7141 tgatacaggt tcaaaaacgg attatccgtc tgtttctcga ttccccgata gtgttttctg
     7201 tcatttcctt ttgcgtccat gttttccatg ccctatttat actccatgtc tttctgttcc
     7261 catcgtgctc tgtcatccac gttcccacag cgcatcgtct gccgcaggcg caggcagccg
     7321 tgacggaact actgtaggat ctcctccaac agtgccactg ccagctccgg attcttcatg
     7381 ccatccgcca gccccacggc tgcggtctcc tgataccatc cggtcttcct tacccattcg
     7441 ttgttctcaa gccggatccc cgccacaatc gtttctcccg tcatggtatt taacaccatg
     7501 atctgctgat cttcatatct gtccaatagt tccttaggca gataatccct taaatccgcc
     7561 agataatccg tgccacccat gagcttcaaa aactcttcgt cagaaaaaaa cacatccacc
     7621 gaccgggtca tgaacaatac ctggatcgtg ctgagattcg tggaatccac atcggaatcc
     7681 gagttcggat cgatccagat cgtatcattc aggttcacca catcccgttt ggatatcaca
     7741 ccttgttccc gcaggaaatc cgtaaaataa tccggaccgg tggcttcgat gtgctctccg
     7801 tccgtattca ctgccatgac tcccatgacg aagctggtct tggtggcaaa atgtacgatc
     7861 aggaaaataa caatagcgat ccccgctgcc acacctatgg taacccacag ataatagtcc
     7921 ataaaatact gccatttttc agaaggagac agttgtttcc attgttcttt ctgccggata
     7981 tggtcttcct tttccaaatt gctgtctccc ttccgtttct cgaatcttcc gtctgataac
     8041 atcctgcttt tagttctata tcagtcttta gtatatcaaa ctaatttaat cttgtatagc
     8101 agacttttta atatatctta ggttcaattt tgttaaaaat aaacagaaaa agccctgcca
     8161 tgcacttaaa ttaacatggc agagctttgt gttttctatt tgttcgatca gataagaagg
     8221 ggaaatgctt tagcggtcca tctccgcaat acgtccggtg gatttgtgct ccttgatgat
     8281 cccctggatc tcctttaagt cggtgcacag caggtcaccg gggatggtgt tcttaagtgc
     8341 gctgatggca ttgccatatt ccagtgctct ctgatactca ccgggatgtg ccaacagtcc
     8401 gtacaggaca ccggagatat aagcgtcacc gcttccgatg cggtctacga cttcaatatc
     8461 ggtgtaaggt gcttcctcat aataggtatc atccttggca ctgtagataa tggagccgaa
     8521 ggtatgacgc ttcgggctat gtacaatacg ctgtgtggat gccacgatgg agattggata
     8581 ctcctccgtg aagctcttca taatgctctt ggcatcgcct tccttcagga aggtcaaacg
     8641 cgccgtgtcc tcggagcaga agaagatgtc cacataaggc agaatggatt cgatacattc
     8701 cttcgcctcg gcgccggtcc acaagttccc acggaagttc acatcgaagg agatgatggc
     8761 cccctgtgct ttaaagcgct tgatcatctc aacagcggta gctctcagct ccgggctgag
     8821 agccagtgtg atgccgctgg tatggaagca gcgggtatcg ctgtagatct tgtcatcata
     8881 gtcatcaacg gtcagcttgt tgatggaggt attcttacgg tcatatacaa tacggggctt
     8941 tctggggtaa gcaccattct catagtagta cacgcccaat ctggcatcct tgtcatcatc
     9001 gtataccaga tagtcatcgc tgacaccgca gagacgtaca cggttcttga tatatacacc
     9061 caggtcattg gccggaagct tggaaatgat accgcagcgc aatcccagca ttgccgcacc
     9121 ggtaatggca ttcagttctg cgcctcctgc ctgcttgctg aagctgtcgc ctctggtaat
     9181 acgctcattg tcgggcggtg ataatctcag catgatctct cccagggaga gcaggtcaaa
     9241 ttccttcttc tcagtcagtt ccataatata aactcccatc tgcatatttt gccaaatgct
     9301 ttatcagaat ttcaccgtta ctccttacag tgcagcgttg aaacccctta cattttttct
     9361 ttacatttgt actatatcac atatgcacgg cctcggtaaa gacactggac attaattaaa
     9421 aatttaacga atccacatat tcaaaatagt catcctcgct ggcaaataag atatattcat
     9481 tctccacata tcccatatat ccgcaggcag tcacatatcc tttcataaaa aatacctcct
     9541 tatcagaatc ctttcgttcc ctgttctgat aagaaggtat catttttaaa gtcccataaa
     9601 actctcttaa ttattctgaa ccggctctgt ttccagcacc aattctttca tttcttccaa
     9661 taattcctcg gttctgccca tcaaaagctc cgcatcggtc tccaccagtc ccgtcttttt
     9721 atacttatag gtaaaaaacg gattgccgta agcagtaaag cttaattcat ccttatgctt
     9781 tttcaggaaa tccggaatct tgcggggatc gatcttcgca tccggccgga agttgaaggt
     9841 aatgcgttcg ttcttgccct tgatctccgt catgtccaga ccgtgggccg ccatacggat
     9901 gagggcgatc cgcagcagat tgtccactgc cttggggatg ttgccgaagc gatctaacag
     9961 ttcatccttc atttcatccc gctcttttat cgtctcgatg ccggcgatcc gtttgtaaat
    10021 atccagcttc tgtgtctcgt tgacaatgta ttccgccgga atgtaagcat ccacatccag
    10081 atcgatcacc gtagcgaaat ccactgccgt atgaatgccc ttcaggttct gcaccgcttc
    10141 gttcagcatc ttgcagtaca ggtcatatcc cacggcctcc atatgtccgt gctgacttaa
    10201 tcccagcaga ttaccggcgc cgcggatctc cagatcacgc atggcgatct tgaagccgct
    10261 gcccagatcc gtaaattcct tgatggccgc cagccgcttt tccgccactt cttttaacat
    10321 cttatcccgc ttgtacatca gaaaagcata ggcattccgg ttggaacgac ccacacggcc
    10381 tctgagctga tacagctggg acaatcccag attgtccgaa tcgtggatga tcatggtgtt
    10441 gacattggaa atatccagtc cggtctctat gatggtggtg gacaccagca catcgatctc
    10501 tccgttaatg aaatcaaaca tgatccgctc cagttcgtgt tccttcatct gtccatgggc
    10561 gtatgcaacg ttggcttccg gcacaagctt tgcgatctgt gccgcgatat ccgcaatatt
    10621 gttcacccgg ttgtagacat agtagacctg tccgccccgg gccatttccc ggacaatggc
    10681 ctctctcacc agttcgtcat tgtattcgca gacaaaagtc tggatcggca gtcggtcatt
    10741 gggggcctcc tccagcacgc tcatatcccg gataccgatc aggctcatat gcagtgtccg
    10801 gggaatgggg gttgccgtca gggtcagcac gtccacgttt tcttttaatt tcttgatctt
    10861 ctccttatgg gccacaccga aacgctgttc ctcgtcaatg atcagcagac ccagatcctt
    10921 gaacttcaca tccgcagata atactctgtg ggtaccgatc acgatatcca ccatgccctt
    10981 ttccagatcc ttcaccgtct ttttgatctc cgatggcgta cggaagcggc tcatcaatgc
    11041 gatgttgatg ggaaaatcct tcattctctg cacaaaggtg ttgtaatgct gctgtgccag
    11101 aatggtggtg ggcaccagat agaccacctg tttgccctcc tgtaccgctt tgaaggcagc
    11161 gcggatcgcg atctctgtct tgccgtagcc cacatctccg cagatcagac gatccatgat
    11221 ccgatggctt tccatatcgg ccttggtgtc agcgatagca ttcaactgat cctccgtctc
    11281 ctcgaaggga aacatttcct caaactcccg ctgccatacc gtgtcttttc cgaaggcata
    11341 gccctcactc tgctgacgca ccgcatacag ctccacgaga tcctttgcca cggcgctgac
    11401 ggcactgcgt accttcgtct tggtgtgttc ccattccttg cnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn ngccacggcg ctgacggcac tgcgtacctt cgtcttggtg
    11581 tgttcccatt ccttgccgcc cagtttgttc agcttcggct tcttcgctgc atcggcggag
    11641 gcgtacttct gaatcacatc cagtccggtg gccagaatgt acagattgcc gccgtcccgg
    11701 tattcgatct tgaggtaatc cttgacgacc ttgtctacct ctaccttctc gatgccctta
    11761 tagatgccca gtccgtgggt ctcatgtacc acataatccc cgatcttcag gtcgttgaaa
    11821 tcattgatct tctgcccctg atagagcttt ttcttctttt tcttacgctt ctccgctccg
    11881 aaaatatcac tctcggacag caccacgaat ttcagccagg ggtattcaaa gcccttattg
    11941 acatgaccgt aataggtcag gatctcccca ggctgtacct cccgcatggg gtcttccgtg
    12001 tatactgccg tcagtccggc atcctcggtg ttccgcagat cctccgccag acgtctggct
    12061 ctggtacggg aaccggacaa cagcagcacc cggtagccgt tctttttata ttttttcaga
    12121 tccttgacca gtgcttcaaa gctgttgtta taaggggcga tgttgcgggc agcaatatcc
    12181 acatgcattt ctttcttgaa atatccgtac ttggtctcca tggtagccag ggtcaccacg
    12241 gcatttttct ctaaagtagc cgctacctgt tccccactgt agagaatgtt catctgtccc
    12301 ggcagcacat atccttttct ggcacgttgt tccatgctct cccggaattc cagttcgatg
    12361 gcatccgcct gttccttcac tctcgccggt tcatcgatgt agaaaacaag agaacgcccc
    12421 gccatatccg ccagcagttc cggcaaggtt accgtatttt cataaaaata gcggatgtac
    12481 ccttccagat tgatcttact ttggaattcc agcagctgtt cttttaattc gctgacctgt
    12541 gtggcgatcc tgtgggcttc ctccggctgg tgggcatccc ggaagatttt ttcctgtttg
    12601 tctgcttctt tttcaagctt actgatgccc ttgcggatct gctcttccga cagtacaaac
    12661 tctgtggccg ggtagacagt gatgctttcc agcttctcaa tggaacgctg gctcaggatg
    12721 tcgaagctgc ggatggactc cacctcatct ccccacagtt cgatacgata ggggttttcc
    12781 tccgtcagat caaagatatc cacaatgccg ccacggatgg agaactgccc cgggctttcc
    12841 acctgatagg ctttctcata tcccatagcc accagtctgg atgccagttt gctctcgtcc
    12901 agactgccgc cccgctcggt gcggatgatg tctttctcct cccacaatac ctgcggtgtc
    12961 atcagtgccg cataggtggt aacaatggtg accggcttac cctccacgac tctgcgcagg
    13021 gtcttgatcc gctcctgcac cagctgattg ccgtggatat ccgcctggaa aaagatcagg
    13081 tccttcgccg gatacagcat gacattcctg tcataaaagc ggtattcctc gtagatctct
    13141 ttggccttta agtcactata agtaacgatg accttgtatt tcaggccatc gctgagtccg
    13201 tagatcatat gaagtttctg ggagtccaca cagccggaca gagctacggt tccttttccc
    13261 tgtctggtca cttttcggat ctcatcatat tccgccagtt ctttcagtgg cgcaatcagt
    13321 gcctgcatcg ctttttcctc ttactataca accgttacgc tttcttactg ttaaaatggt
    13381 tcatggctgc atccgcattc tcagtgatga tcatacggat agcttctgct gcggtcttcg
    13441 ccgcttcgtc catgatgctt cgttcctccg ccggaaaatg tcccagcacg tagtctgcca
    13501 gatcgaagtt cttcggcttc tctcccacgc ccatacgcac gcggataaaa gtatcatgcc
    13561 ctaacatttt gatgatattc ttgaggccgt tatggccacc ggcgctgcct tttttgcgga
    13621 tacggatctg tcccacatcc agactgatat cgtcggagat cacgatcagt tccgcagtgt
    13681 catccacctt gtaataatcc acgatctccc ggatgctctc tccgcttaag ttcatgaagg
    13741 tctggggctt cgccaggatc accttctgcc ctgccacgta gcctttgccg atcagtgcct
    13801 tatgcttact ctccatgacg gcgatatttt cttcctctgc taatctgtct atgacatcaa
    13861 acccgatatt atgtctggta ttttcatatt ctttgcctgg atttcccagc cctgcgataa
    13921 tatacatagc tttctcctta ctttcttgca atcaatgcgt taacttatat tttcgcattt
    13981 ttacgcagaa aatgcaacta ttggttattt tgcacaaatt ttattttcgg gaaaacagcc
    14041 aatttccgca tggcatattt cgttacttat tttccgaatc taaagtatct attcccggca
    14101 ttgtatttta taataatagt ggagtgttac caaaacggta agcggttcac ctttcgccaa
    14161 aatgatgaca ttttgagagc ttccagtatt attggaggtg atacagatgg gaaatctatt
    14221 gaaaggtaca taagatgctg gaaacagtgg taggcattgt cggtattgtt gttacgatca
    14281 tcagtatcat cgtaacgctt atcagtatcg tacagactgc acggaaacat aaacatcaaa
    14341 aaagcaaccg ccctcgccag gattgagttg cttttttgat aagtaactat taacttaggt
    14401 gaaccgttgc tgtacggtag cactcttttc ttttattatc ctaacactct cttttgattt
    14461 cgtcaataaa attcaccatt tacaaaaatc cttgtacccg ggcttgactt tttccggaat
    14521 actggtatac tggtaaggct gaaatgccct gaaatcaagg cttttcggaa atccattcgt
    14581 gtgttcggga ccttccacac gtaaataaaa actaaaggag aaaacaaatg aacaagaaag
    14641 tactgtacct cgtacacgga gcggtgattg ctgctctcta tgttgtgctg annnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaatgattg ccgttctccg cgaaccatgt
    14821 tgtagcatcg ttcattccct ttttgtacag ctttccggtg gacggctcca tgatgacaac
    14881 gttgaattca ttaaatccgg tcaccagcac cgcctcgcca tcctccagga tcgccagtac
    14941 cggaatatcc tgattcacat aatacagtac cgcatccaga ctacagccgc tcagatccag
    15001 tacctgtacg cctgtcatat tttcttccag aatctgtatt gccgtcttgc cctgcgccag
    15061 gtcatattcg gtgttgcggg tgattccggc atgccgcagg atattgtcca gacataccgt
    15121 caggctgttc ttctggtccg tgacactttc ttccttgatt gccatgatct ggttcctgct
    15181 gacacgattg cccttcagcc ataccgtaat gcctcggtcg ttcgcaacaa ccccggagga
    15241 atcgtaggct tccttcaccg ctttcccggg agcactgtaa atgccctgta ccccataagc
    15301 attgtataca taatatctgg aaacttcgct gaccgcatcc agtgtcagtt ctcttccgcc
    15361 ctcaaatacg acctccttcg gattcagcac cttgatagtc ttggtatcaa tggttgtctt
    15421 ggtctgtatc tgcacatatc tctcataaat atcaatatcc gctgtgacta ctttattctg
    15481 tcccggctct tcttccacat tgttcatgat ctggtcatcc aggatctcct gatagctgcc
    15541 gttctcagaa cgctgtattc tcgacagggt gatctgattg ccctcgatgg cgcaatccac
    15601 gatgtagatg ccatcctgtc cgtattcctt cagattatct ccggaagaat tgctgatgca
    15661 gaccttatac atggggtaaa agatctgtcc ggaattctct gtccggatgt cgctctccct
    15721 ggccacgccg tagataatat cttctcccat gaagccaagc ggccggatag cctcaccgtc
    15781 ctccgcccgg atcactgtct gttctccggt attcagattc cggacattca gctggttgct
    15841 gtggttgatg tcatcccctt cctgccatac gatgatctca tggttctccg atacctgaag
    15901 gctgtcatcc tgtcttatac ttaccagctg ttcagcgctg cgattctcca gatccacatc
    15961 atagacaccg ttttccagga aaaaatatac atgttgctgt ctgttccggt acagcagctg
    16021 ttccatctca tccttcagca ccgcatagct cttggaatat gggatataga cttcttcctc
    16081 gatcgtattc agcgaattgt catagctgat gatctgaatg ccggtctctc cctcatgtct
    16141 tccgcgattc atgtaaccat acacggcaaa tttcacatta ccgccctcgt ctacatccag
    16201 gatcttgatg ccatgattgt catacagagt ccgccggtcg gcattatctt tatcatagaa
    16261 ggagaagatc actgtcagtc tgttggtagt cgcattatag ctgagtagct gccccatatc
    16321 ggagaacacc acggtattgc catccgcgct ctccatcata cccacatttt catccgtgat
    16381 tcccagaagg atcttgtcat tggcatacat ccgggtagta tccgggatct gtgtcatggt
    16441 acgctcataa tccagcagat acattctctc cgaagtatat ctgactctgt aatactcttc
    16501 gacattgtag tagatcttat ttttgccctc tccggtggat accacaaaat ccatcagcag
    16561 agaggccaca ttcccactga tctgtgtcag ccggatggaa gccgcgtcct cctggaccgg
    16621 ttccagactg ccccaggtga tctgctgaaa gctgctgtga atattcactt tatgaaaagt
    16681 gccgttatct gtcagcttgg aattcggttc cagatatttc gtcagttcct tggccatttc
    16741 cttgtcgtat aattttccgt ggaaatctgt cgcaaattcc aggatttccg aaatatgcac
    16801 atcatcattc cataaaatag tcgtataata atataccttc tgttcccctt ccagttccag
    16861 gatcaaggtc agactgtatt gtgtattcgt gtcgatcagg tcttttaacg taagggaaac
    16921 ggaaaagctt ttcccatttg ccttccatcc ggtgacttca ctgttctcga tcagccggct
    16981 gccgtcgatg ctgcgaacct ctgtcgagat ccccgtaatt tctcttccga acgtatccac
    17041 ggtgaaattc gcctgtctgc cctcccccag cagagtaatg cagtccttct ggtaagccac
    17101 atccatctcc acggtatttc cgtgcagttc attgcatgcc acaccgccgc tttccatggt
    17161 gatcataggc agagtggccg gagccatctc catggtcata ttgttatgtc cctgattcat
    17221 aatgctgccg atgatcagtg ctgatgccag aaatactaca acacacacgg caattctaat
    17281 gatcgttttc ttcattcttg attcttcact cattcattat tcagcaattt ttccagtgtc
    17341 ggtgccacat ggagcagttc ctccacagta ccgatatctg cgcttttctt cgggcgcata
    17401 ccgttctttt tcactgcccg gatcaacaga ttcttcgggg tatgttccat atcgatgaat
    17461 tccaggatct gggtctcata gccctgttgt tccagcagat ttgctctcag agcatcggtg
    17521 agcagtgctg ccacgcgctc cttgatgatg ccgtatttta acactggttt taaaggatca
    17581 cattttatct gccggttcag ttcatgctga caacagggaa ccgccagaat gactctggcc
    17641 ccccatttta ccgccttgtc taacgcataa tctgtggcaa ggtcacaggc atgcagggtc
    17701 accaccatgt ccgccacatc tgtgccctcg taggtactga tgtctcccct ctcgaacttt
    17761 aatctgtcat accccagctt ttccgccagt tcattgcagt gccggatcac atcggtcttc
    17821 agatccagtc cggtcacctg gatatctcta tgctgtaatt catgcagata atagtacatt
    17881 gcaaaggtaa gataggattt gccgcagcca aaatcaataa tgcggatggt acggtctttc
    17941 ggcagttcat cgatcacatc ctcgataaat tccagatacc ggttgatctg acggaattta
    18001 tcaaatctcg ccctggtcac ccggccgtcc ggggtctgca cgcccagtcc taccaggaaa
    18061 tccactggtt ttccttcctc taaaatataa tgcttggtac ggttgtgagc cagtgcctgc
    18121 agttcctcct cttcagaagc agccgccccc tccgtccgcg gtgccttgcc actgtttttc
    18181 accttcagtg tcagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagccg
    18301 ccccctccgt ccgcggtgcc ttgccactgt ttttcacctt cagtgtcagc ttgccctttt
    18361 tgctgaccag agcggtggcc tgcagggtct tcgctgagaa ttctccctgc cggaactgct
    18421 cctgtaaggc ggtgcagatc cggtccgtca gctgttctgc tgtaaaattc tcgtgaaacg
    18481 cctgagttcc tctgtaagca gtctcctgga acaacagttc ctcccggatc agcaccgggc
    18541 ggatctttac cttctgggtc tgttctggat gacggctgtt gctcaggatg atctgctgca
    18601 gatctttatt caaaattttc tgtaaaagta ttttcagttc ttccatggtt tatcctgttt
    18661 ttcaaatgtt ttgcgcatat tatagcacgc tttcctataa gaacgcaatc attccccgtc
    18721 gtccaccgtg tctccttttg tagatctcat tgcacaccaa cacatggatc agatcctttg
    18781 atactttttc cgcttcctgt tccaggatcg tgctgatcgt atccgctatc cgaaacagac
    18841 tcaggcggtc cggatattca tcatagatca tgcttccttc atagtcgtat ttgtccaata
    18901 tttccgcaat tctgcgctga cagcgtctga cctctccggg ataggtctgc tgcagatatt
    18961 ccagatcatt taagatctcg ttttcatcca ttcttcccgg atagcccgga agtgtcataa
    19021 aaaaaggcct tgataccata tccaaatgcc tcatgctttt ttatagcata tgcgtaaaag
    19081 aaaaaagagg tgctgcgaag cttatcgcag cacctgaatt ctcgccattg ctctctgcaa
    19141 tgccgtctct gccctggcaa tatccgtctc cggcgtcttg ctgcgcagtc tctcttccgc
    19201 acggtgcaat gctgcctccg cacggttttc atcgatctca ttcggccatt cgataatctc
    19261 tgccagaatg gtaactcctt cgggaaggat ctcggcgaat ccggaatgca atgctgcctc
    19321 cttctctccg tccttctccg taatatgcag gatacccggc ttcacgatca ccgtcagggg
    19381 aatgtggccc ggaaggacac caatctgtcc ttccgtggta ttgaattcta ccatatccac
    19441 aattccctca tagaatactc tttccggggt tatgatacga agtgtgaatt ctttatctgc
    19501 catatctgta ctcccctctc atgctatgca tgaacctttg ccagtacatc atcaatgctt
    19561 ccggcattca ggaagtagct ctccggaata tcatcatgct taccgtccag gatctcacga
    19621 aatccacgga tggtctcgct gataggtaca tattttcctt ccagaccggt gaactgtcct
    19681 gccacgaaga aaggctggga caggaatctc tggatcttac gggcacggga tacgaccagc
    19741 ttgtcttcct cggacagctc atccataccg aggatagcaa tgatatcctg taattcctta
    19801 tatctctgca ggatctcctg tacaccgcgg gcagtctcat aatgctcctt acctacgatt
    19861 ctgggatcca gaatgcggga tgtggactcc agaggatcca ctgcgggata aattcccaat
    19921 tctacaatag aacgggacag aaccgtggtg gcatccagat gtgcgaatgt ggtcgcaggt
    19981 gccgggtctg tcaggtcatc cgcaggcaca taaactgcct gtacggaagt aatggaaccg
    20041 ttcttggtgg atgtgatacg ctcctgcagc gcacccatct ctgtctgtaa tgtaggctgg
    20101 tatcctactg cggaaggcat acggcctaac agtgcggata cctcacttcc ggcctgggtg
    20161 aatcggaaga tgttgtcgat gaacaacagc acatcttttc cgcccttgtc acggaagtac
    20221 tctgccatgg tcagtccggt cagaccgact ctcattcttg ctccgggggg ctcgttcatc
    20281 tgtccgaata ccatggtggt cttgtccaat acgccggact cctgcatctc gtggtacagg
    20341 tcgttaccct cacgggtacg ctcacctaca ccggtgaata cggaatatcc gccgtgctcg
    20401 gttgcgatat tacggatcag ctcctggatc agtacggtct tacctacacc ggcaccaccg
    20461 aacagaccga tcttaccacc cttctgataa gggcagagca ggtcaacgac cttaataccg
    20521 gtctccagca gttctgtgtc cgtagactgc tctgcgaagg tcggtgcggc acggtggata
    20581 ggctcgtaag acactcctgc cggtaccggc ttgttatcaa tcggctgacc cagtacatta
    20641 aatattctgc ccagggtatt ctctcctacc ggtacggaaa tgggagatcc ggtggcgata
    20701 gcctccattc ctctcacaag tccgtcggta gtacccatgg caatacatct gacagtgtca
    20761 tcacccaaat gctgggagac ttccactacc agttctcctc catctctggt agggatgcgg
    20821 atcgcgtcat tgatctcggg aagtttgccc tcatcgaagc gtacatccag tacggcaccg
    20881 atgacctggg tgatccttcc tttattctct cctgccattt ctacctcttt cctgcccact
    20941 atgcgggcat ttcataatca actcacattt tgcacttatg tccctgacag gtttgtatca
    21001 ttcctaagca gaccgtaaag aaacgtcggc acttaatgag cgcttcagcg cgaatttagt
    21061 accgctacgt ttcgatccga gcgaaagcgt gtctgcgtgg aagatacaaa gcctgtcaga
    21121 gaaaagcagt gcaaaatata aatattaaag cgcctctgcg cctgcaatga tctctgtaag
    21181 ctcctgggta atgcttccct gtcttgcgcg gttgtacttc agtgacagat cactgatcat
    21241 atcctccgca ttgctggtgg cattatccat ggcctgcata cgggcaccgt tctcactggc
    21301 tgccgcttcc atcagtgcac catagatcag actggatata tatttcggta tgatcatatc
    21361 cagtgcttcc acatcttccg gttcgaaatt catcagcgca tcggcaccct gtgtggatgc
    21421 acccgcctct gcatcatatt ccacgggcag aagcttcaac agcttcggct cgtggctcac
    21481 cgtgttcttg aagtgtgtgt atgccagata aatctcgccg atctctccgg ctgcaaatga
    21541 tgtcagcagt ttgtccgaca gaagcattgc ttcggcatat gtgggctctt cgatgacctc
    21601 cggataggat tccttcacgg tatatccctt tctctccaga atctcccggc ccttattacc
    21661 gatggcatag accacaacgt cttctgcctt ccactcctcc tgtccagtga tcagcttggc
    21721 cacattggag ttgtaaccgc ctgccagacc tcggttggag gtgatgacaa tgaccgcctt
    21781 tttgggggaa gcccccggaa cgagatactt atgttccaga tgacctaccc tggacaggat
    21841 attgtttacg gtatcataca tacagtcaaa atacgtctga gaacgttctg cacctgctct
    21901 ggctctctga agctttaccg tagataccag cttcatggcc ttggtgatct gctgtgtgcc
    21961 ctgaatgctg gtcttacggc gcttgatatc acgcattgat gccatagtta cctctcattc
    22021 tgccgtatat cacggctgac acacttattt cagaaactct gccttgaaat cattcagtgc
    22081 cttcacaagc ttttcttccg tagcatcgga gatcaccttt tcctcagcga tggctgcagg
    22141 aatctcggga tgcttcgtat ccagataatc gaagaactcc ttctcaaatc tggtaatatc
    22201 ctctacaggc acatccagca ggaatctctt ggttactgcg tagatgatga tgacctgata
    22261 ttgtaccggc atgggctggt actgaggctg cttcagcact tctttgatcc gttcgccctg
    22321 tgccagcttc tccttggtat ccgcatccag ttcggatccg aactgtgcga aggaagccag
    22381 ctctctgtac tgggcaagct cggtacggat aggtgctgcg atcttcttca tagccttgat
    22441 ctgtgcggca ccacctacac gggatacgga caaacctgca tttaccgcag gacggaaacc
    22501 ggagttgaac atttctgtct ccagatagat ctgtccatcg gtaatggaaa taacgttggt
    22561 agggatgtat gcagatacat cgcctgcctg ggtctcgatg atgggaagtg ccgtcagaga
    22621 acctccgccg tattcgtcgc tcatgcggac tgcacgctcc agcagtctgg aatgcaggta
    22681 gaatacgtca ccgggatatg cttcacgtcc gggcggacga cgaaggagca gggagagtgt
    22741 acggtatgca gtagcatgtt tgctcaggtc atcatatacc accagtacgt cttctccgtt
    22801 ctccatccac tcctcaccga tcgcacaacc ggaataagga gcgatatact gtaagggagc
    22861 caggtcactt gccgtggata ccacaacggt ggtataagcc atggcaccga actcctccag
    22921 tgtttttacg atattagcca cggtagatgc tttctgaccg atagctacat agatacattt
    22981 tacattctga cccttctggt tgatgatcgt gtcgatcgca atagcggtct taccggtctg
    23041 acggtcaccg atgatcagct cacgctgacc tcttccgatg ggaaccatgg agtcgattgc
    23101 cttgatacct gtctggagcg gcgtgtctac gctcttacga gtgattacac cacttgccac
    23161 acgctcaatc ttacggaatt tgtcagtctg tacggggcct ttaccatcaa taggctgtcc
    23221 taaggagttc actactcgtc caagcaatgc gtctcctacg ggaacctcta ccacacgtcc
    23281 ggtggtcttg acgatatcac cctcttcgat attgctggca cttcccaaca atacgacacc
    23341 gacattatct tcttccagat tcagcaccat gccatataca tcgccgggga actgtaacag
    23401 ctcaccctgc atggcatttt ccagtccatg tacacgagcg ataccatccg ctacctgaat
    23461 gacggttccg acatcggata catccagctg ggaagaatat ctttcgatct gttccttaat
    23521 gacggaacta atttcttctg gtcttaaatt catggatctg tcgcaccttt ctttcagccg
    23581 gtcatcccag ctgaatattt aataattgtt tcttcatatc ttccagtttg gtccggatgc
    23641 tgctgtccac cacccggtca cccacccgga tcaccattcc gccgatcagc gtcggatcca
    23701 catggtaata cacatcaaag gtctgatagg aagtcgtagc cagcagctta tttttcactg
    23761 cctgcttctg ggcctccgtc agctccactg cggtatccac atatgcggtt cccatctttt
    23821 cagattctct gaccagttcg gtaaaataag caaagattgc ctttaatgag ccgtaacgtt
    23881 ccttggtcac cacgatcttc agaaactctg tcagatcatc actgactctg ccacggaaga
    23941 cctgttccac catggagagt ttttcctgtt tggggattcc gggatgtttc atcagcttgt
    24001 caaaatccgg attgtcttcc aatattttct caagctgtgc ggtctcattc atcagctgga
    24061 tcgcatttcc atggtcgact gccgcttcga aaagggcttc tccatagact ttggaaacta
    24121 attttgccat gtatcctcac ccatttcctt cagcgtctct tcgatcaggc tgttctgtct
    24181 ggtggtatct atggaagcgc ctaccagctt acctgccatg acagatgcca cggatacgat
    24241 ctcctgtttg atctcatcgg acattttctg cttttccagt ctggcttctt cctgtgctct
    24301 cgccaggatt ctggctgcct cttccttggc ctgtgtgacg atacgctcct cgttctgaag
    24361 agcccgtctt ctggcatcac ttaagatgtc ctccgcctct ttgtcgatgt ctttcaactt
    24421 cgcctcatac tgtgccttca gttctgcggc cttttcctga ttgtcctttg cctcttccag
    24481 ttctcccctg atcttctctt tacgatcatt taagaacttt ctggccggat tgaacagaaa
    24541 atagctgagt gccaggaaca atacgaagat ggcaatgatc atcaggcagg agtctgccag
    24601 cagctgccag tccaggtcaa acagtcttga catggtctcg ccttctgtta atagtatcat
    24661 tttcatccgc attcctttcg tttaatcagg gaagtaaggg ctttacgaac agcagcagca
    24721 tagcaatcaa cagaccgtac agaccggtgg tctcggcaac tgcctgtccc aataacatgg
    24781 tagaagtgat ctcggacttt gctccgggat tacgtcctac tgctgcagca gcgtgtcctg
    24841 ctgcgatacc ctggcctaca ccgggtccga taccggcaat aactgccaga ccagcaccga
    24901 ttgcagaaca gcctaagata aagttttcat taaaatccat agtaaatcct cctattattg
    24961 agattcttat cagaatcttt ctagcatagt ttcagatagc catacctgaa cagtacagct
    25021 ttaagcgtct cctcttgcat tggcaatgta agtcatggtc agcatacaga atacgtatgt
    25081 ctggatggct ccggagaaca ggtcaaagta tacatgcagt gctgcgggcc acgctgtagc
    25141 gatcacaccc aacagtccgt agatcagtgc catcattacc gttccggaca atacgtttgc
    25201 aaacagacgt aatgaaaggg aaatcggtac tgcgatctca ctgatcaggt taatgggcag
    25261 ccagatcggc agccaggggg gcagcgggga acacatgcct tcccagattt ccttcggctt
    25321 ctggtacttg aactgattgt aatggatcag caggaaggtc agaacaccga gaggcaatgt
    25381 cacgccgtag tccgctgtgg ggggacgcag gcctaacaga ccggaaatat tgctcaccag
    25441 aatgaagatg aagatcgttc cgatgtagtt gtagaacttc ggtgcggcct tgcccatggg
    25501 agcatccacc atggcatcca gcttctccac gatcagctcc accacattct gaaaaccttc
    25561 cggcacttct ctggctttgg ccatctggcg gttggcagca atggcaaaac caatgataag
    25621 caacatgacg atcagcacac acacatgtgt ggtcgttatc catacctcat ggccaaaaaa
    25681 ggaatactta aacacgccat gtatcataaa gtcgatattg tcggcaccgg ataacagaat
    25741 tccatgttcc attgtttcac ctccttattg tttattctgt tgttcctctg tcacttctcc
    25801 atccttcggg gcttccgctt cttccgcagt ctgcggagca gttttgtctt ccgcagtctc
    25861 ctccggcatc gcctggggta tcggatccgt ctcgtggaac agtttattgc ataatttatg
    25921 ggtaagcggc tgtagaaatg ccgttacttt catacatata acataggtga aaaagaaaat
    25981 cagaggattc atcactccgg tcttcatcat caccaacagg aaaaaaatca acatgacata
    26041 tcgtaagaca taggcacgga tgatcatttt cgtagcagtc ttctcgggct ggtccaatgc
    26101 ccggtccagg gaacggtaca tatgatatgt ggaaaccagc gccagcagaa cgccaaacca
    26161 cagacttttg gcatacattg cttcatcctt tgcaataaat gctcctatga tctgcgctga
    26221 cactcccagg aacaagatcc ccagatccat ctccagaacc gttctattta tcttttttag
    26281 tgtgtttatc atcgccgtct tcataaattg tcctggccat ccggtataca ttctgccctc
    26341 cggccacagc tcccatacag aaaagcagga tcatccagaa gtccgttccg aaatgtttat
    26401 ccagccagat gccggctgcg gacatcaggc agatcggtac caacatattg ataccaaatt
    26461 gcatgatcag tgtgatcgcc cgaaacacct gtctgtcgta gggtttcttt tttcttgaca
    26521 ctgtgtcctc ctgtgttgat ttctgctctc agcatttatt tttgtacatt tttgaaaata
    26581 ttttcccact gcagggcaaa cctatagtca ttataacgaa atcttaatat gatgtccagt
    26641 aaaatcctcc tgtccaaatg gtaaaaaatc tataaaatcc tgcgaaaaaa agattgactt
    26701 gaaatctctc cggaagtact atttgaataa ctattcagtg actttacacc cttatagaaa
    26761 gaaggatgac gcgaataaag actacagaca tcaaaagaca gctgcggatc cgtatcgcca
    26821 cccctgagga tgctgcaagg ctggttgcca tttatgcccc ttatgtggaa aatacctcta
    26881 ttacctttga gtacatcgtt ccttccgcag aagaatttgc ggaccggatc cgtcacactc
    26941 ttacccgtta tccttacctg gtggctgaac tggacgggga gccggcagga tatgcttacg
    27001 catccgcttt taaaagccgc gctgcttatg actggtctgt ggagacttcc atctatgtca
    27061 gccgggatct tcgaagcagc ggtgtcggca gtctgttata cgaaaagctg gaggaatacc
    27121 tgaccgcaca gcacgtctgc aatgtctgtg cctgcatcac ctaccccaat cctcccagca
    27181 tcgccttcca cgaaaagcat ggttataaaa ccgtggcaca ttttcacgct tccggttaca
    27241 agcaggatgc atggcatgat atgatctgga tggagaagac gctgtgtcct catacgatcc
    27301 ctcccaggcc ttttatcccg tttccggaac tgtgaccgtt gtaaagcaca ccgcacaaag
    27361 gagaaactct atgagcaacg aacttcagat ctaccgtaaa cggctgatcc cggaagaatg
    27421 cattttatta aaggatgata ttatcgtcga gcagaacgaa gactacattc tgaccaaatg
    27481 gaagaccttg aatcccaaga ccacgttttc ccacggctgt tcctgctatt atctcaagga
    27541 aggcttcaag atcagcaagt tctaccgcca tgacggttct ctgctgtact ggtactgcga
    27601 tattgtggaa tatacttccc gaccggaaga taattccctg atcgtcacgg atctgttggc
    27661 tgacattatc ctctatcccg atggaaggat gcatgtcgtg gatctggacg agctggccga
    27721 ggctctggaa aaggggctga tcacccaggc tcagatgagt gcctgcctgc gccagctgaa
    27781 caacctgatc accattatct accgggacaa gttcgacagg ctgcagaatc cgttggatag
    27841 gagcgggctg tagctgcagt gcttactttt tgctccctgg tcaaaactat tcagagtaac
    27901 atattcgcaa aataaatgag agcataggct cttacggaca caagtgtccg acgagcttct
    27961 gctctcactt actttactct gaatagttca gcaggtttcc gatgcagtgt cccagaatcg
    28021 tcaacagtgt aatatcttca tccgaccact tacggttgcg gttgaacaca tcatagttca
    28081 cgatcacatt cggcactccc tctttgcggg aaagacattg cagggaagca ccgatctcct
    28141 gcttctgcat cacggcaaat gccgccggat cctggaatct gagtttcaat gtgttggatt
    28201 ctataaaatt atcatcatcc ccgaatagcg caagatactt ctcatcttca aaaagtcctc
    28261 ttccatccgg tacttctacc atgtaattac cattccggca taccagctgc gctcccgact
    28321 ctgaataaat cgtgattcca tccagatcaa acagactgcg gatctgctgc agccgttctt
    28381 ccttttttac aatggagttc acatcggata cacacagttc ggtgatcatc ttcgtgatga
    28441 cttcccgctt tttctcctta gataccgcat aggccatagc catgttctgc atatcgtcgc
    28501 tttccagctt gccgtgaagt ctctcttcat agatgatatg gcggttcttg cctttttcct
    28561 tggccagata gagtgcctta tccgccttgc gcatcagttc ctcgtattcg cttccatcta
    28621 ccggatactc cgatacgccg atagaagctg tcacatgggt cttcggatca aatgccagag
    28681 caaattcctt cgcaatggtc ttcaaaagtg tcttcagctg tcctctggtc tccactttct
    28741 ccaacaggat cacatactcg tctccgccga atcgtccgat cacgccccgc tgtcccacca
    28801 gcacctgcat gatctccgcc actctccgga tgacctggtc accaaaaata tgtccgtagg
    28861 tatcattgat atgtttgaaa tcatcaatat caatgatcac cagccatctg gtactatgat
    28921 ccttgcggtt cagctgttcg atggtgtact ctgtccatgc ccgcttgttc agcagaccgg
    28981 ttccggcatc tctggctgcc ggagtcagat aataggcttc cttctcccgg tttctgtccg
    29041 gggttatgat gcccgctacc atatcacgct tcttcgggat ccgtgcgcct ttgaccttcc
    29101 atattccctt gtcctcctgc ccgctgacga attccatctc gaagaaatcg tccccgttac
    29161 gcagatgtgc gcaaaaggtc cgcagctgat cttccagtct ggaaatatca ctgccctcat
    29221 gtgcctgttc cacattccgg atcagcgcat tcataggccc ttcaaactgg ttcatggccc
    29281 gctcgttgac atatttatag accgtaatac tgtcctgttc gatcagatat tcaaaaaagt
    29341 aaacatcttc caatgtcata aaatgacggt acttcagaat gctctgctcc agtcgtaaat
    29401 tccggttctc cagatcatga atatctgaaa cggtgatctg atacaacgga atcccgttct
    29461 ccgtctgttc actctgttcc agacgcagat acacattcct tacactgtct ttcagattgt
    29521 gaagcaccgt gatcagttcc agcggttgga acgtatccgc ctccactgct tttttcaaca
    29581 gctccccgtc ttccggcaga agcagctcac tcaccggccg gaaagaattc tcccctacga
    29641 attcatagaa aaattccgac gccttggcta ctaccagatc ctgccttacg aagatcttcc
    29701 agtccatgat tctatttgca taaccctttt gttctacgct gttgactctc atagattttc
    29761 gctttcgttt aataaaatac atgtccgccg atattgatac ctgtcagtcc ttccaccggt
    29821 gtgcggaaat atacacagtt tcctacattg gtcacaccgg acatagcctc gtctgccgcc
    29881 tggtagcagc ggctggtggc tttattggtg gccaatgcca gttccagacg tccgctggcc
    29941 accggtgaga actgtttgtt ctgatagatc actcctacca ctgtgttggg ataaacagaa
    30001 cttctgacac ggttgataac cacactggct accgccagct gaccgtcata gggttctccg
    30061 cccgcttcac aatagatcag atttgccagc agataacggt ctccctcttc aaaggagata
    30121 tccgaaatgt cccgccaggt tgcgtttgcc gccttctggg acatggcgat ctcttctgcc
    30181 agctttttcc ggagtgtttc cagattttct tctttttgtt tgatctcctg ttcatatgcc
    30241 agggctctct gctctgcttc cgaaatatcc ccggcatatt ttgcaatgct gttagatgtc
    30301 tggctgatca gaccggacac cttgcttttc tcggcttccg ctgccatctt cagactatcc
    30361 agctccgttt tctctgcctg cagtctcgcc tcttcctctt cgatcagctt gcggttctct
    30421 tcgaattcct gcagtttctt ctggtcatag gctgcgatcc gttcaaaaaa gtccgcagcg
    30481 ttcagcagtt tatcgaagct ctgggcattc agcaaagcgt tgatgtaact ggtctcattc
    30541 cgctcgtaca tatcccgtac ccggatcacc atacactcat actgccatgc ttccgtagct
    30601 ttcgcctctt ccagactggc ctgggtatcc gcgatctcct gctccttgtc tgcgatctgc
    30661 tgttccagat ctgacagatt gttactgact tccgtcagct gaccgttcag attattcagt
    30721 tgtcccttga gggaattctg ttctcccttc aggttctcca gattatcatt ttcctcattc
    30781 agctgattct gcaggttgtt tttctcttcc tgctctttct gaatctgctg ccaggtactg
    30841 ctggtagctt ccactttgat atcttctgaa gaaaaggctc ctgcaaggat cactgctgta
    30901 agcagcacag ctgtcttctg taggcgcttc aaccgtttta ctccttttat ctgttgcttc
    30961 agacctcgat gcgtatcatg cggcccgtct tacggtactg cacgtaattc actccggccg
    31021 cgtcaaacat ccgcttggct gcccggttgg acggagttcc ctcatactta tcgctgtcat
    31081 ataccaccgt gcggattccc gcctggataa tagctttcgc acattcattg caggggaaaa
    31141 gagagacata cagcttcgtc ccctccaggg aaccgccacg ataattcaaa atagcattca
    31201 gttcactatg tgttacaaag ggatactttg tcagattcct gtcattgtcg ttctccctct
    31261 cccaggggaa ctcatcgtcg gaacaaccct tggggaagcc gttgtatccc atggacagga
    31321 tcttgttgtc cgggctgaca atgcaggctc ccacctgggt attcgggtcc ttggaccgca
    31381 ttccagacag gtgggccacc cccataaaat attcatccca gctgatataa tcgctgcgtt
    31441 tggcactcat gggtgtctcc tttatttggt accgaaaata cggtctcctg catctcccag
    31501 tccgggaaca atgtatccat gatcattcag cttctgatcc agtgctccga tatagatatc
    31561 cacatccgga tgtgcttcct gcatagcttt tacaccttcg ggtgcagcga tgatgcacag
    31621 cagacgaatg ctcttgcaac ccttgtcctt taacagctgg atggctgcga tggaagaacc
    31681 gccggtagcc agcatgggat ctaccacgaa tacctcacgc tctgcacagt ctgcaggcag
    31741 cttgcagtaa tattctacag gctttaaggt ctcaggattg cgatacaggc cgatatgtcc
    31801 tacttttgca gcagggatca gggataacat accgtctacc atacccagac ctgcacgcag
    31861 gataggtacc agtgccagct ttttaccctt taactgcttc acagtggtct tgcagatagg
    31921 ggtctcgatc tctacttctt ccagaggcag atccttggtt gcttcataac cgatcagcat
    31981 tgcgatctcg ctgatgttct gtctgaagtc cttggtaccg gtctcctttc tgcggatgat
    32041 tccgatcttc tgctcgatta aggggtgatc cataactact acttttgaca tattgttgtc
    32101 ctcctgtgtt ttgtatttgt tataatggta tttcgttgtt cgtgtctgtg taatgcatta
    32161 aacctcttcg atcaggctga ttctgcggct gtgtctctct tctccggaaa aaggtgtatt
    32221 caggaaagtc tccacgatct ccagcgccag gttctcaccg acaatgcctg cacccattgc
    32281 cagcatgttg gcatcgttgt gcagtctggt catcttcgcc gacagaggat cggagcagag
    32341 tgcgcagcgg atccccttta ccttgttggc agaaatgctg ataccgatac cggtggtgca
    32401 gatcacgatg cccttctcgc aggtaccgtc tgccacagcc cttgccgctg catgtccgaa
    32461 cacagggtaa tcgcagcttg ccttgctgtc acatcccata tcctgataag gaatgttctt
    32521 ctcctctaaa tatttcttta ctctttcttt cagatcatat ccgccatgat cacttgcgat
    32581 tgctatcatt ttccttctcc tcgtattttc tgtttaaagt tttactactt tatggcccgc
    32641 tgctttcaac agccggttca taatggcctg tccgaagcct tccgtggaaa atccctcgga
    32701 atagatcttt gtcacacctt catcatcgaa ttctctcaga atagtgtaca gatgtctggc
    32761 gatactgtcc tcatccttac ggctgccgac acttttcacc acatctgcta tataaccgga
    32821 cagcatttct tcggttccga tgattccggt cttctctcct gcggtatggt ctgctgccgc
    32881 agcccggtta atatactccc ttacctgccc cagggttccc tctacgatgg tgagttctcc
    32941 cctgggtgca taatgtctgt acttcattcc gggggccttg ggtgcctgtc cgctgtctgc
    33001 ccgcaggatc gtggtgtccg tatccactgt tcccagcact gtgctcagtt tctcctgggt
    33061 aatgtatccg ggccgcagga tctgtggcgg tgtcaccgtc aggtcaataa tggtggattc
    33121 cagaccaatc cccacctcac cgccatcaat gatggcatcg atccgcccca tcatatcttc
    33181 ttcgacatat tttgccactg tgggactggg cttgccggaa gtattggcac tgggcgccgc
    33241 aatatatccc ccgctgtatt tgatcagctt ctgtgccgcc ggatgcgagg gcatacggat
    33301 tgctaccgta tccaggccac ctgtggtctc gtaaggcacc gcgtttgatt tcggcaggat
    33361 catggtcagg gggcccggcc agaaggcatc cgcgatcttc cgcgcctctt ccggtacctc
    33421 ctttgtaatc ttgtaaatgt cctcaaaccg ataaatatga acgatcagtg ggttgtcgga
    33481 cggtctgccc tttgccgcat atattttctg ggaggacgaa ggattcagtg catctccccc
    33541 cagcccgtac acggtctcgg taggaaaagc caccagtcct ccctgctgca ggattgctcc
    33601 cgccgcccgg agtttctcct catcttcttc gctgacgggg ccctctccgg gacgaatctt
    33661 tatgatctca gtctccatgt ttttaccttc tgtgttctaa attgcatttg gatatagtat
    33721 atcatatccc cggtcaaatg gaaagattat tcctctccgg tctgccggga tgttgtgtgt
    33781 acagaataaa cggtccatcc gaccagcagc ggaatcaccg ccacggtcgg cagttcatag
    33841 cggattgcat tggacatggg ggatgccagg cacaccaaca cgtttataaa tccgggaacc
    33901 agcagtatca acgcactgta tcttttcttt ttccacagga aaaatgccag cactaccaga
    33961 atccaggtat acaatcccgg agtacacaga tacttgatca gtggcagccg catactgagg
    34021 aaccagatct gcagcagaaa ctccgatgtc tgaatgggaa atgtatgatg aattccctgt
    34081 tcctgcgcat attccggata ctccagctgg atccatgcct ctatgttctg ttccaccggt
    34141 gccagatagc catagccctt gttgacaaat gccgccacat agcatcccgg atgcttgaaa
    34201 aacatctgca cccatatttt catgtagggc cccaggtctc cgcctcgata taatatttta
    34261 atgggatccg aaatcctggg ttcataattt ttcatagcat caaagttcag ggtatcattg
    34321 atcacctgtc tctcatattc cgtaacctca tcttcgtatt cgcagacata tctcgccgtc
    34381 tgctggaacg gtacgctcag cccctcggaa gctgccggtt ttcccacatc gatcaatgct
    34441 ctgtatccag ccaccgtgat cagttcatag atcaccacag ttgccagcaa taccaggaat
    34501 gccttcctgc gttctttgcc tttcagataa attgccagca aaagcagtgc cggaacaatc
    34561 gcatagatcc cgttatttct aagcaggcag gataatacac tgaatacaat cagtcccgcc
    34621 gcatctttcc cgccacattc cttctttttc agtaccgaca tcagatatac tgcctgtaac
    34681 accgtcattt ccgcatacag cagatctttc tcaaaccatt gggcataggt tccccaaaaa
    34741 ggtgtcaggg cgaaaaacag gattcccacg gcacaccatt tccaggaaat tcccagttcc
    34801 tgcagttttt tcatgctgag ggaaaaagca taggccccta ccagtgtctg cagcaggcaa
    34861 tacaggaaaa agccgaaatt ggcatctgca atggttcttc ccagcacaaa acagattccc
    34921 ataattgcgg aggaaaatgg cggatgtgcc gctccccagt cctgttcacc cagaaactgt
    34981 ttcagttgca ccagaccgtc cccactggta gttcccggat aatttagcag gatccagaac
    35041 agccagctga aggctattac aaaaaagccc accatatgta ggtgcttttt gccctgctct
    35101 gcgggtaatt ccacatgctc cagcccaaaa tataccaggc gcaataccag atacaataac
    35161 actgcaaacc ccaaaacaca gaacgtggat aatagtatct gaaaagaatt gccccacagg
    35221 aaaatggcag aatcatattt cttaaagctg atacatacta taagaaggaa tgacagaata
    35281 acagaaagca ccaacgttgc cacatctgtc ttcgcctctc ttttcttcac ttccttaagc
    35341 attacaaata ctgccgcagc cgttaacaga tctgtataat ccagcaccgt aaatgctccg
    35401 cacagtttat tcagcccatt gacaagattc gaatccacgt atcctcccag gcagacttca
    35461 aatgtacatt gcaatgccag tgtcacgatc acggtcaaca gaacatttct tattttttga
    35521 tatcgtctca ttttctctcc cgtaacttta taaaattctg tcataacatt cgaatcctat
    35581 attatcataa taacactgtg tatacaactc gtgcctttat tttgtatttc ccgtttcaat
    35641 ccctctgtat cccgttaatg tactgtaaaa ttcctttgtg gatcgaccat gccactctgt
    35701 cctgataggc ctcgtcacac agttttcccg cctctgccgc attggacagg aagccgcatt
    35761 ccacaatgac gatggttcct ttggtttttt tcaaaagata atagctgtcg ttgggcttga
    35821 tctgccggtg gttctccgga tccagatcct ggaccagctg tgtctggatc agccctgcca
    35881 gttgttcccc tccgctgctg ccattataat agaagacctg cgctccgtgc acatactctt
    35941 ccggatagct gttctgatgg atgctcaccg tgagaaccgg atccgtctgc tcaatctttt
    36001 cgatccggcg tttcatatcc tgcactttct tattgggagc agatgcatca tacaatccat
    36061 cctccgtctc tctggtcagc accacccgga ttccgtctgc ctccagatac tgtctcagtt
    36121 tctctgtgat ccggagattg atatccttct ccggtactcc gttgatacca atcttccccg
    36181 ggtcatcccc gccatggccg gcatcgatca ctaccgtcgg tattccgtcc gtgtccgcat
    36241 tattcccctg caaggcattc acggcattta ccgtttccac atatgccgcc gtttccctgc
    36301 caagtatcgc catagacata agcaacaggc ataccatcac cgcccttaac actttctgtt
    36361 ccatccctgt tctccggtgt ctcttttatt ctatatgatt ttatatgcct ttttctctgg
    36421 aaatatcttc cgaaaattaa gaaatccttt tgttttaaac tattgcatcc ccttactact
    36481 tcagcgtata ataccattat gaaaataagc aaaaaaactc tttctatcat tttatttgtt
    36541 ctggcgggaa tttgtgttct tgctgcagtg atcctgttgg taaaagggcg gatcgaagac
    36601 cggcgtatgg aggaagcgct ggatgaattg cgcccgacgc agtccgcgga gaccacgcag
    36661 gcaccttcca cagtcgaagg tgagccgacc gttaccgtct ccccggaacc gtccacagaa
    36721 gagaccccgg cttccaccga ggagttcgaa cgagtggcca acccttatgc cgacagtttt
    36781 cttgccaaca aggatatggc ggcctggcta cagattccgg gcaccaatat cgattatccg
    36841 gtaatgtgga ctccggaaga tgagacctat tatctctacc gtgcttttga cggcagcgaa
    36901 aataaaaacg gctgtctgct ccttgacgat gaaagctgtg tagatcctct gaccacgaat
    36961 cttatcattc acggtcacaa tatgaaatcc ggtgccatgt tcggcaatct gaccgattac
    37021 gagaatcagg atttctataa ggaacacaaa aacattatcc tctacaccga agagtgtcag
    37081 cgcaattacg agatcattgc cgtctttcgc tcccaggtct accggaaaac agatcaggta
    37141 tttaagttct acaaattttt ccaggcggat acgcaggaag aatttgatga cttttataac
    37201 aatatcaagc agttatccca gtatgacacc ggtgtcaccg cagagttcgg agatcatttt
    37261 ctgacactgt ccacctgcgt ataccatgta gagcagggcc ggttcgtagt cgttgccaaa
    37321 gaagtggaac ccggtgatca ctaccttccc atacaggaat aactcacaga caatcaggag
    37381 gttttcttat gaagagactt aaaaaatttg tcacactgtt cacgctggct gccgtatgct
    37441 ttgctattcc cggtctggga aagatcactg taaaggctgc ggagcccacc acctatgttc
    37501 tgaactattc cgacagcagt tccgagtgga gatacaagga agcatccagc tggtccgcag
    37561 aagttcagga ccgcgagttg tattatctgc agcagaatat caaagacggt gactatattg
    37621 tcatcgacaa cgatgtggag aacaatgccc tggccctcaa ggtttctgtc cgtctgggca
    37681 acctgacctt caagaatact gttggtgttc ccgtagtcta tgccaacggc tatgacagtg
    37741 tctatttcct gagcggtacc tccggtgctg tcaacggtga tgtatctcat gcctatgtat
    37801 atggcgatgc caaggcaaat ttcaacagca atgtagacac tctggaaatg atcggcctga
    37861 cagatgataa atccaataat ctgcatgcca ccatcaacgg tgtcggtacc gtcaatcatc
    37921 tgattgccaa agacaaccgt gaccagagcg ttttctatga ggcctacagt ttcgccgcag
    37981 gtaagctggc cattgaagac ggatccctga agaccgaagc aaagtattac agcaccaccg
    38041 ctcccgctgc tgccgcagaa gtagctcctg cagctaccga gcagcccaca cagaccacag
    38101 ctcctgcaca ggccacacag aacagtactt cttccgaata tgacaacgtt ccaaagaccg
    38161 gtgaaaattc tgcaatgttc tggcttctgg gtgccgcagt tgtatgcatg gccggaggct
    38221 ataccatccg cagaaagaac gtacaggaag cggaataaag ttttcggcag caataaagcc
    38281 cagtcccttg cgggaccggg cttttactat tttattctgt tcctactgca gataggcatt
    38341 gatcagttcc caggctgcct gtgttccgta acccagtctc cataccgcca caccaccaat
    38401 attcttggta gccatcatgt tcagcttcac tgcgatggat tctgcatctt ccagccagat
    38461 ctgataggtg gcatttccgg aggtccattc tgcataattc tgacaggtct cctcatccca
    38521 ctctgctgtc acaccggctc tgctcatgaa atctgcctcg ttgttcatgg taatatattc
    38581 atccgtcaca tccgtgcctt cggtcttcca caggatcgtg tagaacggaa gggcattgac
    38641 taccttgttt gcaggcacct cctgcaatgt tctgtccaga ccgccggata catagctaat
    38701 gcttgccacg cttccgggat ccttgcttcc gtgccagtgt tcgtcatatc ccatgataat
    38761 gacatagtct gcaacctgtc cctggatatc cagacggtaa taatcattga aattaaacgg
    38821 tacatagttg gcaatggaca ataccagtcc catgtttcgg cactgaatgg aaagttctcg
    38881 tataaattcc acataatgcg gtccgcagtc actgctcagc tgctcgaagt ccacattgat
    38941 gccgtccagt cccaggctga ctgctgcatc tgtcacgttc cgcacaaagt tctgccggct
    39001 ggtggtggag gacagcatat taagagtact gatggccgtt ccggtctcat tggcgtaatt
    39061 aaaattatcc accacgcccc atacctgtaa gcccatattg tgggccgtgg ttacataatt
    39121 cgctgttccg aagttccgga tacttccgtt ctcatccgtc agactgaacc aggtcggtgc
    39181 aatgacattc atacccttcg taccggacac catggagtac agagtgtcat taccgcctgc
    39241 accgccgata gaatgccagc ccaggcagat cttgctgtca gaagtcaggg cagtatattc
    39301 cggctcctga taatccgtca ccggagtctc ggtctcttcc gtaatatctc ccagacgctt
    39361 gttctccaca tatccgatca cggaatccga tgtcttcacc ttactccagg tctccatctg
    39421 ctccagaatc tccagggtat ctcccttcgc tgcctgcgtg aggatcgggc tcttcacacc
    39481 gccccgcaga cgcaccgccg tatctttttt cagctgtgcc acctgtctgg tcccccattc
    39541 ggtgtagacc tgcacatgtc tgtcataaca atcataggaa taatttgtga acagcttcac
    39601 ataatccgcc gccacatata cggtatcccc ttccacgaaa ctgatcacat atccggcatc
    39661 ctgtgtgcct tctgttgtcg tataggaagt ctcgccgaaa gtcgtacgga tcacttctgt
    39721 cggattggcg tacaacaaca gtttttccgt catatccgca taaaacactt cgttcatata
    39781 tttatggaca gttgccagat caaaatagca tacgtcatct ctgatcagtg cctgttcttc
    39841 taccatttcg tcctgtaaaa taatggcact tctgtcactc tctgacacct gataaaattc
    39901 gttccagtct gcctcttctt tgctgtagga atatttatcc aacagcacct ttcccagtgc
    39961 tccgcccgcg atcacaagga tcagcaggat tgctatgatc accggtagta ttcttttctt
    40021 catatgtgct cccatccgca ggctgccgcc tgcaccattc ttgccttttc ttccgttccg
    40081 tcttctatcg gcagaacagt tttattctat cacaattcct ctatataata caaggaacaa
    40141 tagagacaga tcctgcactg cgggatacag ccagagaggc aatcccgttt tgcatccccg
    40201 cagcgcattc tctgtttcaa agtattaccc gggcaagagc tgacaccctt taacgcagat
    40261 ccaccttatc cagccggacc tggctgattt tgccctgttc tcccaatata tagtctgcca
    40321 tatacgggct ttcttctctg acggcatacg ctgccgcctg cgacggtgag accttttctc
    40381 cgtcgacata caattctgtg ccgtttttct gaagctttcg aaaatatttt tccacctttc
    40441 tcctgggaaa cttcttcccg ggagtcacag cttcccggtc ctccacagac acctcccgtc
    40501 cgcagcatat tttaccaaaa ggattcgtga aacactttaa aataacgctg ctttttccgc
    40561 tagtacatat aagcgtaact attcagaaca ctattcgcaa tataaatcat gtcaaatgtt
    40621 cttacgaatg caagcattcg acgtacatat tgacacgatt tgcgtggctc tgaatagtta
    40681 cttataaatg cctctgacag tacttgtagt aatttgtgtg aaaatgttgg aattatgttc
    40741 gtgaaaatta tctgttgaaa tattatgacc agacacgtat atgcctgtaa gacatctcca
    40801 tactatattt tacagatcag cataacctat ccttcccaaa tacattaaca gccatctgca
    40861 tcataaaata aaaaagggga tgaactggaa cgaatcgttc cctggtaaat caacaagaaa
    40921 aaatggaaaa ctgtcgacct cctctccgta ccggagtatc agaggcatct ataggtcgga
    40981 ttatacactg cagcctgcta tttgacaaga tgttttatta aattttttta aactgtgaat
    41041 agcttctatt gactaaaata tagggaaagt ataagctata taccatacca atgcgaaagc
    41101 agaaaggaag tgttgtcgca tgaaaataga aaaagtaaat gaaaatcaga ttcgctgcac
    41161 cctgacccgt gaggatctcg ccagccgcga attaaaaata agtgaacttg cctacggcac
    41221 agaaaaagct aaatctctgt tccgtgatat gatgcagcag gccaattttg aatttggctt
    41281 cgaagcagag gatattcccc tgatgattga agccatccct ctgaatgcgg attgtatcgt
    41341 actgatcatt accaaagtgg aagatccgga agaactggac acccgttttt cccgctttgc
    41401 accttccgtt acggaagaca gtgatgatga cagtgatgag agcacggcag acgaggttct
    41461 ggatctgttc cgccgtatcc agagcgaaga caataccgca aacgctcctg aagctccttc
    41521 cgcgtccgct cccgaggaga gtgacagtgt acgttcccgc ctgttccgta tggaatctct
    41581 gaaacaggta atcaatgcgg cagtaatagc cggaagccat tatcatgggc tcagcactct
    41641 gtatcacgaa gagggtaacg acgggtatta tctgatcctg acgcagggcg aagaagatcg
    41701 cgaggtcttc gaccgtgtct gcaatgtcat ctctgaatac ggtattccca agcgttccac
    41761 accggcaagc cttacttttc ttgaggaaca ttgttccacc ctgatcgccg aacatgcttt
    41821 acagcgtctt tctaatactt caatgtaaca accgatcata acattcagga gagttcccac
    41881 tctgtgggga ctttcttatt tttatggttg ttttatacct gtctctccca gcaagagtct
    41941 gtagaaaaga agccgggaaa cgcgtaactt gaaaatatag tcttattcgc atataataaa
    42001 atttaatata tgaattgctc cgttgcaatg cagcttacgc aattccatac acattcggac
    42061 atacatattc ggaattacaa agggtcaaaa cgccggttac agtggaaagg atgtacacaa
    42121 tatgatttcc gacgaagaat tataccgaca atatttaagc ggcgatgaaa caggnnnnnn
    42181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatatga tttccgacga agaattatac
    42301 cgacaatatt taagcggcga tgaaacaggg ctggaagaat tgatgagaaa atacgacaat
    42361 ccactgacct tatacatcaa cggctatctg catgacgttc acgaggcaga ggacctgatg
    42421 atagaagtat tttcctacct gttcgccaaa aggccccata tccgggatgg cggattcagg
    42481 gcttacctgt ataaagctgc gcggcatatg gctttgcgcc ataaaagcat ccggcgacat
    42541 cacttctgtc ttgatgatct gaccaaagag ccggaaggtg gaaaactggt ggatgaggtg
    42601 atccggacaa aagaacggaa tcagatcctg catttatgca tgggtgaact gcatccggat
    42661 tacagagaag ctctttatct gacctatttt gagggtatga gttaccggca ggtcgctgaa
    42721 gttatgggaa aaagtgtgaa gcagatcact aatataatgt accggggaaa agagcgtctg
    42781 cggggattat tggaacggga gggaataaca aatgcggagt catgaggaac ggatcgcaga
    42841 agtccgacga aggattgcgg aaaaagagcg acaaaaaagg cggaaacagc ggcggatcgt
    42901 tgctatttcc tgcatagtgg catgccttgc tgtgatcttc ggggcttcct ttgtcatgtc
    42961 cgggatttcc gggcaaatgg tgccggggat ctcctccgga tatgaaacgg cggcaacgat
    43021 ccttggcggt ggcgctgcac ccgaatatat cataataggg ctgctttcat ttcttctcgg
    43081 cgtatgcgtg acgatcctgt gttttcgtgt ccatctgttg gacaaagaag agcaggcaga
    43141 gcagaggggg gaccgcgcca atggaattga tcattaatct tttgcaattt ttcgttacca
    43201 tgctgggctt ctgtctgagc ggcattcgtt atctgagaca tcggacactg tcatattttc
    43261 tgctgatttg cttttatggc tgtttcgcac tgggctccct gtactggaca ttgtatctgt
    43321 tattgttttc cgaaacacca cggatctttt atgtatcgga attcggctgg gtctccagcg
    43381 ttatttttct ctgtctccta caatatactc tctcctcccc ggaggaaaga agcttttcct
    43441 gtcggaaatc cctgctggct ccgctgatcg gagttccgtt gtgcgcattt tactgtacct
    43501 ttggtgacat tctctccaac ctgctctggt gcggcatgat gatccttgtc tcctatcgtt
    43561 ccatccacgg tcttgtctac gcagagacac agacgggaaa aggacggaac ctgcggtatt
    43621 ttcacatcgg aatcctctgc tatgttgcga cagaatacgc tctgtggacc tccggctgct
    43681 tctggccgga cgactctatc ttcagtccct attgttggtg tgatcttctg ctgacgttct
    43741 gccttttcgg gctgctgcct gccacaggaa aggcggtgcg gacatgacct atattgaaaa
    43801 tgtatttatc tgtatggcct cacccctgtt gatcgctgcc ctgtgcatgg gtaggcggca
    43861 ggctaaattc tttctgtgct gtcttgcagg aatgggggca tgcctgctct ccgcttacat
    43921 caataccttc tttgccgctc tttacgcagc agatacgatt gccgccactg cggagatcgc
    43981 acctgtggta gaggaagtga tgaaactcct gccgctgctc ttttatcttc ttgtatttga
    44041 gcctgaaccg gtgccgataa aaaatgctgc catcatcgtt gcactttcct ttgccacctt
    44101 tgagaatgtc tgctatctga ttcaaaacgg agccggacat ttttccttta tctttttccg
    44161 gggcattgga acgggtgcca tgcacgtaat ctgtggtgcc atcgtcggcg gtggactggc
    44221 gtatgtatgg cagcgcacct ggctgaagat cgccggaacc tgcggtctgc tcggggcagc
    44281 catcactttt catgcaattt ataatcttct gatcgcctat ggcggtgcga tacagtatat
    44341 cgcatacatt ctgcctgtgc tgatcctgac ggcagggaaa ctgatcaccc gccgccttac
    44401 ccagaatcac aactgaatcc tgtcttttgc ggagctgctg tcggcagctc ctgttttttt
    44461 gaaaaaattt gattttcagg tgagagtttt ttcgcttttt tgtacctaat tagatgaaag
    44521 gcatatgtat tttatgactt tactttccga agggaggttt tcaacgatat gtatgataca
    44581 gaaaagcggg tcgaactggt gaaaaaacgg gtgcatgaaa aatatcgccg gaagaagcgc
    44641 cgcgggatct gccggctgtc tgccctgtgc acggcattgt ctgcgttcct tgtgggaacg
    44701 gtgcacactg cagccggcca gacgcagatc accgcccggg gaatgtacgg ttctatcctg
    44761 ctgcatgaag acgccggcgg ttatgtgctg gttggggtaa tttctttctc cacagcagtc
    44821 atgatcactg tgctgtgtat cagactccgg gaaagagaac accgcggaca aggcacggga
    44881 aaaggaaacg catcacaaca atgggaggaa aaaaattcat gaaaaagcaa aaaattcggt
    44941 tctatgcggc gctgctttgc agttccatgg tactttctct ggtctccacg ccggtatcgg
    45001 cagcagaaac cggacagctg acgaatcccc tacatccaca gagggtccgg gttccccgga
    45061 aagcgcatcg ggaaatgaag ctgcagcgat tctgaacgga ctgtacacaa cactgccggt
    45121 agcaaacggt gtgaaggaag tggcgacagc ggaggagctg gcggctgcac tggcagatag
    45181 cagtatttcc gggatcacac tgaagggtga tgttgaaatc agcagtactc tgacagttaa
    45241 ccgcactgtg acgctggatt tgaacggtaa tgtactgaaa atgaccggcg gcttcagcgt
    45301 aatcaaggta gagtcgggcg gcgatctgac aattgcggac agcactccca acaaagtaca
    45361 taaatttaac ccgaactata cagatatgtg gggatgtggc ctatggaaac tggacaaaga
    45421 caccggcacg gaaatcgtct ctggtggggt tattacgggc ggtggcggtg attttgccca
    45481 cagtgatggc ggcggtgtgc ttgttaatgt cggcggtaaa ctgaccatga ccggtggcag
    45541 catcgtcgga tgctcggcag gtggtctcgg cggcggtgta caccttgcct atgactcttc
    45601 catcggcaag agcagcacat tcaccatgac gggcggaagt attatcggct gcgcggcaaa
    45661 aaacggcggc ggtgtgtctg tctccccggg gtgcacattc accatgggtt ccggctctga
    45721 gatccgcaac tgcaacgcac aatccggggg tggcggtgtg gacatcagcg ccctgtggaa
    45781 ttcaaatatc atcggctgct ttatcatgaa cggcggaaca atccgtacat gcacaggatt
    45841 atacggcggc ggtgtttaca actctggatc ctttattatg tcaggcggta cgattaaagc
    45901 cagcatcagc accaccaccc agtatgcttc aagcggcggg gtgtggaatg ataatcagtt
    45961 caccatgaca aggggcacga tcggtgatcc ggataataaa aaagacccaa gccatgtata
    46021 caatacttcc actcagagag ttactcttac tatgcgcgac aatgcaaaaa tctacacaaa
    46081 tgtaacaaac gttggcatcc tgaatgctga cggcggcgaa atgtccggca caatgacgaa
    46141 cgataccaac aggtacggca ccggcacgat caccggcagt gagggtgccg ccggcagcac
    46201 tgagttccat ggcaaagtga ccaacaccgg caccatccgg aagggcacat tcaagaatga
    46261 ggtcataaat gagagcagtg gtaccattaa cggcggcaca ttcaccggcg cgattacgaa
    46321 caatgatggt acggttttag acggtgattt cagcggtgcc accctgaacg gtatgctcgt
    46381 gatcactttt gacccaaaca atggggatca gcccagcaca cagaaagtga attggagtaa
    46441 ggacggagcg gcactcaccg caccggatcc cgttcccacc aatgaggggc atagtattga
    46501 aggctggtat tatgataaca acggtacgga aacaaaatgg aactttgaca cggatacagt
    46561 caaatgcact atgaccctca aggcaaaatg ggaactctcc acctattccg taacgctgca
    46621 gacagacggc ggtacgattg ccagcggaaa agaagtaacc ggctacactt acggcaccgg
    46681 ggcagttctt cctaccacca atgatattac ccgcgaggga taccgctttg acggctggta
    46741 cgcagacagc aacttttccg gttcacccgt cacagagatc acaggtaccg atacggaaaa
    46801 taaaacgttc tacgcaaaat ggacgaagaa cacgactccc atcatctccg gaaacacgat
    46861 caattatatc gtggagcact acaagacgga cggcagcggc tacacgctgg cggaaacaga
    46921 acattccgca gggaagacag gtgataccgt cacagcaacg ccgaaaacct atgaaggctt
    46981 tacctacaat ccggcaatct ccaccgccag cggaacgttg aagaagatca gcggtccgga
    47041 ggatatcgtg accctgaaac tctactatga tatgaatgct gacaccgaac aggaaagcac
    47101 cgacaacggc cccgaggaaa aggctgaccg tgagaatccc tctcctgtcg tgaaaaaaaa
    47161 cccctcctat atgacctata ccgtacaggc aggtgacact ctctgggcaa tcgccagaaa
    47221 atacaactgc tccataaccg agatcgtggc agccaacagt gaccggatca agaacccgaa
    47281 ccggattcac gccggctggc agcttaagat tccccaaagc ggagcgccta tcacaggcgg
    47341 cactcccgat gctgtcctgc ctgagaataa gaaaagcggg atatacatcg tcagacaggg
    47401 cgacaccctc tgggcaatcg ccagaaagtg tggctgctct gtagccgaga tcatctccct
    47461 gaaccgggaa ctgatccgca atcctgctct gatttattca ggctgggaac tgaaagtacc
    47521 acaggattag cctcccctga aagaatctac cgtgctagtc atacttcata atatcggatc
    47581 agataatatc taagggaaaa aggccaaaaa aaagacggcc aaaggccgat gatctgaatt
    47641 ccttgaattc ttttgatttc tcaaatccgc gcaatcgacg gacacatttc gattgcgcgg
    47701 aaatctgagc tatgaaattc cataaatact ttgtttcacc tgtgtgcccc ggtcaggcga
    47761 tgtttccaaa acagaaatac aaaaattaca tctcctcgat cactttcata agctcatcta
    47821 catcaatgcc atgtaccata gctgcctctt ccagggtctc tccctgtgaa gcagggcatc
    47881 ccaggcaatg cattcctgca tcgaggagta cgggagctac ttcaggattg gcacgtaaga
    47941 tctcaccaat ggtcatgtct tttgttactc cggtcatatc ggtctccttt ctgccgtata
    48001 aggctttttt agtataatac tttcccccat attttacaag aatattctta atacatcagc
    48061 tctgacataa aataccggat ttctgacgag ggtgcttgca catagagtca gataatcggt
    48121 attttatgaa caaagcgctt ttatgaatga ggaagctttg ttgctgatgt acaaaactcg
    48181 gaacaattcc taaaaaggtt gacacttacc cttgtcaggt agtacacttt tatcgtaaag
    48241 aaacagcttg ggataccagt gaaaccggta ctctcagttg ataatattaa ttgtttttac
    48301 cagaaggagg taaattttca tggcagaagc atggagaagc tttaaaaaag atggccattg
    48361 ggatgtagac gtcaacgtac gtaacttcat ccagaccaac tacactccgt atgatggaga
    48421 tgagagcttc ctggagggac ctaccgaagc aactaataag ctttggggca gacttcagga
    48481 gttacagaag gaagagcgtg agcataacgg cgttctggaa tgcgaaacag aggtcgtatc
    48541 cagtctgacc gcttatggcc ctggctatat tgatgaatct atgaaggatc tggagcagat
    48601 cgttggtctg cagacagaca agcctttaaa gagagcattc atgccttatg gtggtatcaa
    48661 gatggctcag caggctgcaa gcacctatgg ctacgaagta aacgctaaat atgacaaaat
    48721 cttcaacgag taccacaaga ctcacaacca ggcagtattt gatgcttata ccgatgagat
    48781 gaaggttgct cgtcataccc atatcgttac cggtcttccc gatacctatg gtcgtggacg
    48841 tatcgtaggc gactacagaa gagttgctct gtacggtatt gatttcctga tccagaaaaa
    48901 ggaagaggat aagaagaact gcggtaccgg cgttatgacc gaggacatca tccgcctgag
    48961 agaagagatc gctgatcaga tcaaggctct gaagggcatg aaggaaatgg ctaagattta
    49021 cggctacgat atttccggtc ccgctaccac cgctaaggaa gctatccagt ggttatactt
    49081 cggttacctg gcagctatca agacccagaa cggtgcagct atgtccgtag gacgtatctc
    49141 tactttcctg gatatctata ttgagagaga cttaaaagaa ggcaccctga ccgagaagga
    49201 agctcaggag ctgatcgacc atatgaccat gaagttccgt atggttaagt tcgctcgtat
    49261 tccttcttac aaccagctgt tctccggcga ccctgtatgg gctactctgg aagttggtgg
    49321 tatcggcatg gatggtcgtc atatggttac caagaacgac tatcgttttc tgcatactct
    49381 ggagaacatg ggacccagcc ccgagccgaa cctgaccgtt ctgtactcca gcgctctgcc
    49441 tgataacttt aagaagtacg cagctaagat ttctgtgaat acttcttcca tccagtacga
    49501 gaacgatgac gttatgaagc ctatctgggg tgatgactac agcatctgct gctgcgtatc
    49561 cgctactcag accggtaagg agatccagtt cttcggtgct cgtgctaacc tggctaagtg
    49621 cttactgtac gctatcaacg gtggtaagga tgagaagcat ctggacaaga acggcaagca
    49681 tatgcagtgt ggtcccgaaa tcgctcctat cacttccgag taccttgatt tcgacgaagt
    49741 tatgcataaa tatgacatca tgcttgactg gtgtgcatct ctgtatgtta acatcctgaa
    49801 cctgatccac tacatgcatg ataagtacta ctacgaagca gctgagatgg ctctgatcga
    49861 taccgatgta agaagaacct tcgcaaccgg tattgcaggt ctgtcccatg tagctgactc
    49921 cctgtccgct atcaagtatg ctaaggttaa ggttatccgt gatgaccagg gttgtgctgt
    49981 agacttcaag atcgaaggcg acttccctaa gtatggtaac gatgatccca gagctgatga
    50041 attcgctgta tggctgctga agaccttcat ggagaagatc aagaagaacc acacctacag
    50101 aaattccgag cctaccactt ccgtactgac cattacctct aacgttgtat acggtaaggc
    50161 taccggtgct ctgcctgatg gtagagaagc ttacaagcct ttcgctcccg gtgcatcacc
    50221 ttcctacggt gctgagaaga gcggtctgct tgcatccctg aactccgtag ctaagctgcc
    50281 ttatgagtat gctctggatg gtatctctaa tacccagacc atggctcctt ccgctcttgg
    50341 tcacaacctt gacgagcaga aagaaactct ggtacgtgta atggatggtt acttcgatcg
    50401 tggtgctcat cacctcaacg ttaacgtatt cggtaccgag aagctgttag atgcacaggc
    50461 tcatcctgag aagcctgagt atgctaactt caccatccgt gtatccggtt atgctgttaa
    50521 gttcatcagc ctgaccaagg agcagcagga tgannnnnnn nnnnnnnnnn nnnnnnnnnn
    50581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50641 nnnnnnnnnn nnncacccct cagctgcctg cacggcagag aatggagatt attacaatga
    50701 tcaaaggcag aattcactca ttagaatcct tcggcgcagt agacggaccc ggaatccgct
    50761 atcttatttt cctcaagggc tgcaatatgc gttgtcaata ttgccacaac gtagatacct
    50821 ggaatcccga cactgacaat ctgatgacag cagacgagct tctggataaa gcagagcgtt
    50881 tccgcagcta ctggggcaaa gaaggcggca tcactgtcag cggcggcgaa gccttattac
    50941 agatcgattt tctgatcgat cttttccgca aggcccacga gagaggcatc aataccaatc
    51001 ttgatacttc cggacagcct tttaccagag aagagccttt cttctccaaa ttcaatgaat
    51061 tactgaaata tactgatctc gtcatgttgg acatcaagca tattgatgat gaagaacaca
    51121 agaagctaac cggccatacc aacaagaata ttctggactg cgcaagatac ctttccgagc
    51181 agggtattcc catgtggatc cgtcatgtac tggtgcccgg aattactgac aatgacgaat
    51241 acctgaaaaa gacaagagaa tttattgata ctctggatac cgtcatgaaa gtagaagttc
    51301 ttccctacca tactctgggt gagtacaagt ggaaagaact ggggattccg tacaagcttg
    51361 agggtgtaga accgcccacc gaagagagga tccagaacgc gaagaagatt ctggagttca
    51421 ataaatataa ataagcaact gtaactattg ataaaaaata ctaaataagt cttgtattca
    51481 aagcccattt atattacaat acaagtggtt atattgataa atttgtatca aacaagagat
    51541 ttgatacacg caaaatagga ggaaaagatt atggcatttg ttattggtga tgcttgtgta
    51601 gcatgtggag cttgtgaggc acagtgcccc gttggcgcta tcagcatggg cgacggaaag
    51661 tttgaaatcg atgcagataa gtgcatcagc tgcggttcct gcgcaggtca gtgccctgtt
    51721 ggtgctatcg cagaggatta attcctgatt cagaacgtac cccggggaag gcatttcaga
    51781 aaatgaaatg ccttttcttt ttttctgcct taatttccct gccggtattt tctttgtctg
    51841 cgatctctgt ggactctttt tccactttcg gccttctctc ctctttctgc tgtttccgca
    51901 gttgtcccct ggtctttttc cgcaacagct cgtccatctt ctgatagtac tcctcgctgc
    51961 gctgttccag tctgcggtct ctctcctctt cccgttcttc tctgctgcgg aactgataat
    52021 ccagctcctt gaccatgctt tccttgagtt cccggcaaag gtcctggtta ttctcccgta
    52081 ctgcctcacg gatcaactgt tgcaggatcc actggagcct tttagccttc tcttccctgc
    52141 tttcactctg cagtccttcc ttctccggca ccgtacatat cctctcctgc ggctgtaccg
    52201 gcatctcttt atccaccggt ttatcaacga tctcgatggc catgtgtggt gtccggactt
    52261 ctgccggctc tttccgggct tctacaggct ctttttcccg ttcctccggc agtacgtcca
    52321 gccgtccgtc ctttaatatc attttaattg ctttcaactg taatcccctt tctttcatgc
    52381 tcttgatctc tttgaaccgt tccacgtctt ccttcgtata atagcggtgc cccagctcgt
    52441 ttcttttgat gggcagatgt aattcctctt cccaatagcg aagtacatgg ctctccacct
    52501 ttacctgttt tgcagcatcc gaaatcaata aatactctcc catattctct tcctttccac
    52561 atagccatat ataatgatgt cggggtcaaa agcccccgac tttgatgcct acggattgtt
    52621 ccgtgct
//
LOCUS       DAWEJW010000005        50238 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_338_length_50238_cov_63.366957, whole genome shotgun sequence.
ACCESSION   DAWEJW010000005 DAWEJW010000000
VERSION     DAWEJW010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 50238)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 50238)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..50238
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_338_length_50238_cov_63.366957"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    29429..29528
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        12497 a        14090 c        10359 g        13192 t
ORIGIN      
        1 gtcttcttct ccggtgtact cttcctcatc agcatagtac tcttcgccgt cttcttctcc
       61 ggtgtactct tcctcatcag catagtactc ttcgccgtct tctccggtgt actcttccgc
      121 atcggcatag tactcttcac cgtcgtcttc tccggtgtat tcttcctcat cggcatagta
      181 ctcttcgccg tcagtctctt ccggatactc ttcttcgtcg gcatagtatt cctcgccgtc
      241 gtcttctccg gtatattctt cctcgtccca atcgtaacca tcctgtacgt cttcttccca
      301 gttacgatcc ggacgttttg tatggttaaa taattttttc atctgatcta tcccttctgc
      361 tcataattct cggtgtaata attccgtaac attatagcat tgtattttcc ccacatcaag
      421 gcattcaccc atttcttcag aatttcttaa atctttctaa tattatggac tttcttttcg
      481 tataatagta aaatcaatat aactcttttt accactacat tattgaatta cctgaaacgg
      541 agagaataca tgaaacgaaa caataacaac cataatatcc acgcatcttc tgtgaatgac
      601 atgccggata ttgagattgt agatctggaa aacgacacaa tgcaagacac agattctggt
      661 aatttccatt ccgggaatac tcgttccggt tctgtgcaca atttccatac cgattccgat
      721 gacgacatag aagaccttta cgtagatgcc gatgaagaaa tccctgaggg cgaagagtct
      781 gcggcccctg ccggtatccg gcgctttctg aattttcacg tactgtttgc tctcgtgctt
      841 cttattgttg tcggcgtggt aggataccgc gtcacacact ggggacagag gatcagccag
      901 tccgacatct tcaaggacgg gcagggttcc tacgacgaca gttgggattc catccttccc
      961 ctgaccgacg aaaacggtca gatgatcatt aatgatgctt ccaatatcgt tgtcttcgga
     1021 aatgctccct tcgcagatga cagggactct tccgacaatc tggccaatct gatcgccaaa
     1081 gagaccgata ccaccgttta taactgttcc atcagcggaa gttaccttgc tgcacagcag
     1141 ttaaactacg accccaccgt tgcccccatg gatgcttact gtctctactg gctggtgaac
     1201 ctggccgttg gggttcctct ggatggctac tacaccgatg cagccaatgc tctgggcgac
     1261 aagacaccgg ccgaggcaga ggaagtaatc aacacactga aaactctgga tttcaacacg
     1321 atcgacacca ttgccattat gtatgatgct accgattatc tgtccggcaa tgccatgtac
     1381 agtgatgaga atcccacgga tcccacgcag tttaccggca atctggaggc cagcattgaa
     1441 gtattgcaga actattaccc ccagatccgc attatcgtta tgtctccgac ctatgcatat
     1501 gctgtggatg agaacggcga ctatgtaagc tccgatatgt atatttacaa cggccgtgat
     1561 gtcttatcca cttatgtgat caaggaatgt tactccgcca atatccacag cgtatccttc
     1621 atggataacc tgtacggcag cattaccgaa gacaatgccg atgaatatct tgtcgacaat
     1681 ctgcacctga atgtaaaagg acgtaagctc atcgccaaac gttttgaata cttcctgaat
     1741 tactattcca aggattatgc atcccaggaa aaataattcc ggtcaaaaaa ggctcccgaa
     1801 gcagccagtt actttgctgc tccgggagcc acttgttttt ccctgaaatc tcattccttc
     1861 ataaagtaat acagatccac tgtggaatct ttgtagatca catatccgtg cattcttccg
     1921 aattcttcaa attcctgatc ctgcatcttc cccaccagtt ttttatcttc cgacaggatc
     1981 acataggcac aggcctcttc tctggccagt tctgccagct cctgtgcatc aatgacctcc
     2041 tgctccataa catcataaag atcattctgc accgtccact tgctcaccat aatgtcccgg
     2101 ccgtagggca tacaaatcac attggaatac tgtctcacat actgcagcag ttctcccggg
     2161 aatgccgcca tgacttctct gcccggaacc tcaatggtat cacagatatc cacaacgctc
     2221 tggggtacat gatacgcatt ttccgccttc tgaaaattgg gattcctgta gatcagactg
     2281 ccggatatcg taaacagcac aatagccaac gttatcccca catatctttt tctaccggcg
     2341 agttttccac aaagctgcac cgtaccgaaa gcaatcaccg gtgtcacagg caacaaccac
     2401 aggatccggt aatagatttc cccatccgcc atctgagaga cgatcttcgc caccagcgga
     2461 ttaaaaaaga caagcagcaa tactaacggc acataaagaa acatcacccg gatatatttt
     2521 cttttctctg tgagccacag atacagcaga ctcaccagga accacagaac gatcagtccg
     2581 gtccccatat attcctgaaa cagctttaaa gcatccattc ttctcctccg gtcctatcat
     2641 cccaacagca aatacattcc ggcatacaca atgcagggaa tacaacagcc gatcaatggc
     2701 agtatgagtt tccattttct gtaactgacc gcaccacaga gcccggccgt tcccaccagc
     2761 atgcatgcca gcaatgctcc catggtactg gccagacagc aggccgtcat cacagatccc
     2821 aacagcaccc agaaaccgac agtgatcttc tgttcttcct gtatttttcg aagcagtgtc
     2881 agcagcagga aaaatatcat ggggatcagg atacttccca atgcagcctt cccctgtctg
     2941 ctcctggcaa tcataaaatt ctctacagta taataagaat aatctccgaa aagcaccagc
     3001 acttccgtac agagtaaaaa tgcaggcaac gcccatttct tccggtatgc gccctctcct
     3061 gcgaataata ccctgcttcc cagaagatag aatactccgt aagtcatgga gatcagagcg
     3121 accggcacca gagtcttcgc cacgatcacc gtagtgatgc cggtcatctg ggccagccat
     3181 gcgatccaga tgggaaaagg tgccagcccg tgtctcacat ccagttccgt atgcattccc
     3241 gtataaggga tcttctgata catcctgccg gattcctctg ccgctgcgga gattgccaca
     3301 taataggcat cgtcaccatc accgtagacc atcagcactg ccaataccag ctgcaccagc
     3361 aaaagcacac cagccagcac ccacaaaata atctctgctt tctccggctt tgcttcccgg
     3421 actgcccgca gagtacggga ctgccggaaa taccggaccg ttgccgctgc cagcagcacc
     3481 aggatgtagc cgcaataagc tgcggtcacc atccacatac ggccttccga caggatcacc
     3541 ggcacacaga tcacctgaaa tcccgcccat agcagcatct gaccgctaat ccacagaaat
     3601 accggatttc tacagggttt atccacattt aaaaaacaat ttcccaccca tgccggtatc
     3661 gctaccagca gaacggcaag ccacagcaaa cgaactgcga tcatgtttca acctcatttc
     3721 tgtcttctta tcatttcatt ccttatctgg aaaacatata tatcatatct ccgtgcacgc
     3781 cttccgaaag cagaagttcc tccgacgtat aaccatactt atcccggagc acagacactg
     3841 ttgcttcttt atccacaccc ggtttgatca atacgatcac ccggtccagt gcctccacgg
     3901 attccgtatc cttacgttcc ttcagttcct ctgccgttac cagcaaagtt ttggtgtaat
     3961 gcataaattc cggcaggttc tcataatagc ccactcccgc ataaacgcag atgcatggga
     4021 actcctcata ttcttccgcc aatgcttcct gtctggcata tccctgatag aggtattccc
     4081 catcataggc cgcaagccgc agcccctgta ccaccacgag caacagcacc agcatgcctc
     4141 ccacgatctg ttctttccat ccggagatct ctgtcagttt ttttccaagg agacacaata
     4201 gcaaagcaag cagcaatgcc acaaacggaa acaaaggcat aatatagcgg tccaccagat
     4261 acggtgacat gcgggatgcc agcagaaaat atccggccac cggcaccatc atcatccagg
     4321 ccgcatctct tccggaattt tcacctttct gttcctgttt cccggcccgg aagctcagat
     4381 acagctttcc cagaagcagg attgcgccgg cggcacatcc gccggtcaga agaggcgctc
     4441 ccactgtccg atccgccaga attctgccaa atgcagccag tctctgtccg taaccggcaa
     4501 atccggaggc cagattgccg agagcttccg tccctcttcc gctggagaac acatccgaaa
     4561 ttgcaaaagg gaacaatacc agtccgatca ctgcagcgat caccatggac cggatatagc
     4621 accataattc tttgttcctg tgccggtaca gcaggatcac tgcggtaacc gccgccagca
     4681 gaaggcagta aaacaggaaa aaatactgtg tcaggaaacc cagcaccgta atagcgataa
     4741 ggcccttgtt tttcttgtca aacccgttat tcttccattt ttccacatgg acggaaaaca
     4801 gggcgacaca cagacaggaa agcagcccat acatacggat cagcagcacc gtacccagtg
     4861 ctccggtact cattccatag agaagcaccg ccaggatccc cagctgtctt ccctttcctt
     4921 ccagggaaaa cacatctgcc agctgtctcc ccagccgcag taaaagccac aatgtaattt
     4981 ccagacagac cagattgatg ctgcatccaa gccacggtga caatgttccc cgaaagacag
     5041 aactcatggt atgcaacagc ataaaatgta ccggcggatg attgtcatct tttacattaa
     5101 aataaaccga caggtaatca aaggcatctt tctgatccac cgtgacataa tcctgaaact
     5161 gccggtccgt gatccagacc ggttcctcat agacatctgc tttataagac aacagcttgc
     5221 tgtttccccg gttcctaagc acatctgtta ctgtattttt cagattctcc agagtctctc
     5281 ccgcactgtc cccctcgatc tcctcgttca cgaatttggc cagacgccct tccggctggg
     5341 taggtacgat ccagggatta tacctggcat ttgccagttc aaagctcaga agttcgtcca
     5401 tgtgatagcc ttccttccgg ctgactcccg cacccatcaa taccaggcac aatataactg
     5461 ccagcagttc cggcaggatt tttttccatt tatcccgttt ttcctgtcca atcataggaa
     5521 tcttcccatc tccgttatta ttcattatcc attacccgat agagataaat gcggtaatag
     5581 ctgttctctg tctcaaaggc ctccggcctc atcagttcca gaccggtatc ctcactgtga
     5641 tcgatatagg ctgccgacaa cagatagctt ccgcccagct gcctcagact ttccgcatcg
     5701 atcgtatagt cctgaaaata aaagccgcct ttttcgatcg tataatagcc ggggcattcc
     5761 gcgctgaaca aatagcaacg attcccccaa tggtcaaagc tgtcttccag atattcactc
     5821 ttgtccagct ccggtgcaat gatctcccgg aaacggtgct tgtactccag agaatagtta
     5881 tttgaatagc cgtccagaca gtaaaatcca tgatacaatg ccgcagcggg atcgatcccc
     5941 agacttacga cccggtaatc ctgtggttct tccccggtat tctcccgcag gtactcctgt
     6001 acctgatcca gcacatccac ggcataataa tcccggaagc tcatggctgc ataattccgg
     6061 ttcaccagtt tttgcagatt gggcttcaga ttgctctcca gcaaaatctt cccggctatt
     6121 gccacggtaa gaagcattgc ggccattacg atgatccccg ggatcacacc gttcctgcgc
     6181 ccgttccctg ttttctccgc tcctgtgtcc ctttccggga gctgttccgt cagcaataac
     6241 aggctgcacc ccagaataaa ataccacagg cagggactca gccacaggac tctgttggcc
     6301 tgaaagccct tcagagcacc cagactggaa cggatagcga ttccgatact gctgtcccac
     6361 agtgcggcca ctgccgcaaa gcctgcaata acggccagtg acaggcccac ggctttccat
     6421 aaccgttttt ctccgccctc aggtacattc tttttatccg ctatgtcctt cttcgttgcc
     6481 cgatttagaa aaaataaaac aactgttgtc atcaacagca ccacgaggat cagtccatga
     6541 taatccacac tgtgctgtcc tccctgcagg aggtttgttt tcaactgact gaaaaagtcc
     6601 accgggctga gcagatattc cgatttgtga gaaatctctt ctccctgtcc tccggacagc
     6661 tgaagcaata acgaaccgtt ctcaacgata tacgtaagca gcaaaatccc ccaggctgca
     6721 gcctgtccgg cggagaactg tttccttttg tccaccagtg tgacgatctc ccataccgcc
     6781 cagattccca gcacagcaaa accggataac accagggaag aattacatcc gaaaagcagt
     6841 acataaaaat aacagagaat cctaaaattc ttcggtctgt ccttttcccc cagtcgcagt
     6901 acacaataca ttaacagcgg caatccatac tgggacagcc cgtacaccgg caaaaacggc
     6961 agatacgcat acatacatcc ggtcagcatg gctatgaaac cgacaagccc ttttcttctc
     7021 cgcagtgttt ccgatgtttc tccggcccat ccaagcaggc agaacattcc cagatatccg
     7081 gcaaagctgc cagccacctg catacaggcc agtgccagtt ccgcaggcag ccaccggtat
     7141 aacagcacac aggcgggggc cggcatggta agcgctgttt ttgctgcacc gttcatgaat
     7201 tccggcaata caccgctgcc atcccacaga tgtcttgcct gcagaagata ggcgatcact
     7261 tcaccgtcca gctgatcgtg atacacgaac acagagtcct tccctaatac aagatacgga
     7321 actcccataa aaaatactgc caacgctccc agaaagaacc aggctttttc tgatttctgc
     7381 gttttcactt ttgttgtcat cttcgtcatg gttcctcctt cccgcgtttc tgctgtcaca
     7441 attctattat ttctccgggt tgtcttctgt atctccggcg cttatggtcc tatcggtcca
     7501 gaggatcatt cccacaagcc acagagaacc cggcagcaat ccgttcttgt aatccaggca
     7561 tccggcatcc cgcatggtat accacaggat catgcctgcc gcagtgatca cacagactcc
     7621 tgatatcttc cagaacaggc ttctgtccgg tactgttttt tcttttttct gtcggagcat
     7681 ggcaatgatc ccgcatatca tgccgctcac gaccacaaca gcgattcccg acaggaacca
     7741 ccagctgctg tattttaaca aaagctctgt cagccttgga tctcccgtca tcataatgtc
     7801 tacatttctg ccagagaagg agtcatatcc cctgccgtcc aacagcagct gcaacagtac
     7861 cgtaggcatg gcatatccga ggcaatccca ggcgatctga tggatattgt cggaagtata
     7921 cgtcttcaga tgatactcgg catactctct gaatatcttc tgtgcttccg ccttgccgaa
     7981 acgatcctcc aacgtctttt gtatgccgga ctccatggtt cccgcataat acccggcatt
     8041 cctcatctct ttccaggtca tccaggtacg tagctgttcc ggccattcat cgtattcttc
     8101 ttcctctctc atatgcgtcc agctgaaccg gcgcatcatg gcggcgctca ccgtattctc
     8161 cgccttccca tagctgcccg gtgtctgcca catgggcacc agcgtgaata aaattcccgc
     8221 cattgccaga aggagcaaaa gttcccggcc tactctcttt ttctccgtct ttctgctccg
     8281 tagctgccac agatgtatca gccacagaac aataagcggt accagcccca gatacagatt
     8341 ctccaccaca cacagtgtac tgagcgccca ccagattccg gcttcgaaca gaccatgtaa
     8401 cggtctggcc ggcgctgtcg tctcaccatc ctccgccaca gcatcctgcc gcaggatccg
     8461 gatcaccgcc gacagttcca gcagtaaaaa ggaatatccc ggagaattcg gcaatacggc
     8521 cagatgactt tgcatggcac agggaaaggt cagaagcgcc aggcttcccc agcacaaaaa
     8581 tatctttttc cctgtcactc cgcatgcccg taaaaatact acaccggcat aaaatgcaat
     8641 caggatctgt accgtatata caaaaaaagt ataagggata gaacttatgg attctatccc
     8701 ttttacaagc atcagaaaaa gagggtagcc gatccccatg tagtcatcac acacaagtgt
     8761 ctctgacgct ttcagccatg tgtagctatc gccgaattcc ggaaaaattc caaaggatct
     8821 gacgccccag cagattccca gaagaatctg cacggagaca cccaggaaca gaaatctgcc
     8881 tgcaacctgt gcagacgctc tgccaatttt ttgcatgtta ctgctcctct aattcaaaaa
     8941 agtttttcac cagtccacag atgaaatcca cctgatccag tgtcagccca tagtacatgg
     9001 gaagtcttac caggcgctcg ctctctctgg tagtataacg atcctcgccg tggaaccggc
     9061 cgaacttctg tcctgcggga gaagtatgca gcgggatata atggaatacc gtaagcacac
     9121 cgttctcctt catatagctg ataaatcttg ttctctgttc cagatctctt gccttaatgt
     9181 agaacatatg cgcattatgc acacagcctt ccggtacggt gggcaggtcg atctttcccg
     9241 ctttctttaa gggaagcagc tgctcataat agcggttcca acaggcaagt cttgcctcat
     9301 tgatctcatc agcgatctcc agctgtgcat agagataagc cgcattcata tcactgggaa
     9361 gataggaaga tccgtaattt acccaggtat atttatcaat ctggccccgg aagaacttgc
     9421 tgcggttggt tcccttctcc ctaatgatct ccgcttcttc cacgtccttc ttatcacgga
     9481 tcagcagtgc tccgccctca cccatgctgt agtttttggt ctcatggaag ctgtaggcac
     9541 cgaagtcacc gatggttccc agtgcttttc ccttgtaaga tgccagtaca ccctgtgcgg
     9601 catcctctac tacctttaag ttatgtcttt ttgcgatgtc cataatggtg tccatctcgc
     9661 aggccacacc ggcatagtgc accggaacga tggctttcgt cttctccgtg atggcatcct
     9721 cgatcagtgt ctcgtcaatg ttcatggtat ccgggcggat atccacgaac acaggaaccg
     9781 caccgcgcag tacaaaggca tctgcggtgg atacgaaggt gtaagcaggc atgatcactt
     9841 cgtccccggg ctgaatgtta cacaaaagtg ctgccagttc tgtcgcatgg gtgcaggagg
     9901 tcgtcagcag gcatttaccg gctccggtcc tctcctcgat ccaggcatta cattttttcg
     9961 tatattctcc atctccgcag atcttctgat tttttacaca ttcctgaatg taatctgcgg
    10021 ctttatccac ataaggcggt acgttaaact taatatcaat ctctctcata acacatcctc
    10081 ttttctactg tcttttagct ttctctatgt tcccgcttcc agcttcctac agctgtccca
    10141 ggcacatttt ccgtcacatg actgataata tatttcggtc ttcccttgac ttcttcatag
    10201 attctggcaa tataatggcc aatcacacca aggcttaaca taataaatcc gccaataatg
    10261 aggatcagca ggatcaccgt ggtaaaacct tccaccgccg ttcccatcag atagcgcacc
    10321 agggtctgaa tcgccaggat cacactgaac aggatggata ccccgcccat gatcgtcacc
    10381 agctgcaatg gcagtgtgct gaaggaagtg gcattggtaa tggcatattt catcagactc
    10441 cagaaggacc acttgctctg tccgtatttc cgctcctgta cttcatattc cacagtctcc
    10501 gtacggaacc ctgcccagaa ggtcaacgcc cggaaaaagg tattgcgctc cggcagttcc
    10561 aacaggacct gtactacttt tctgtccaga agtttgaaat cggaagaagc attcatgtcc
    10621 atcttgatca gtttgctcat gatcttgtaa aaaagtccgg cagacaactt atatcccaga
    10681 ctctccttac cgcggtcggc cttgatacct tccacgatct cggcaccctg ctgccatttt
    10741 tcccacatct gtggaatcac ctcaggcgga tgctgcagat cacagtccat aacgatcacc
    10801 gcatcccccg tagtcagctc caggcctgca aaaattccag cttcctttcc gaaattccgg
    10861 gaaaactccg ctccccgtac tctcggattc ttggccgatt cttccttaat acgttcaaag
    10921 gttccgtcct tcgaaccatc actgataaat accagctcat aatcgatccc gtgctgttcc
    10981 agcagttccc ccagtacttt tgcggtattg gcaatattct gttcttcgtt atatgccggt
    11041 aaaacaatcg ataataatga catcctctaa tcccttccgc agttctcggt cttgacatac
    11101 aggattccat ccacaaccct ggtggaattc gctcccggaa aagtatccat gtcaatgtat
    11161 tcctgcgtca tataaatctc acccacagta tccggatcca gaaaattcag ggttgcgccc
    11221 agatagttct gcataaatgc ctgataatcc gctcccttat agatcagata gtctccgctg
    11281 agaccgatca taccgtccgt gacttttccg gtaatatccg tagtggggaa ttcatcatac
    11341 ccgatgacac ccaccaatgc aatgggaatt ccctgatagt acccttccgt ctgctcgatc
    11401 ctgtccagca gacgtacaca gtacgcatag gtcttctcgt attttttctc cagattggaa
    11461 tatccgatat tatccgatac tccataattg aataccagga ttccggccgc cagtaatgcc
    11521 acccactgga tcccgatatc tgttttcccg tcctccgacg catatctgtc cacaaatgcc
    11581 agcatcagga tcagatataa tacccactga taccgcatca gcaaatgata ggtcagattc
    11641 ggagaaatca ccagaatcac attggtcaaa agcggaagtc ccgccgccaa cagaattatt
    11701 ataacgaaaa atgccggatt cttccaccat tttcttcgca gcatgcttct caccagcaga
    11761 taagccacca gaagcaccag tgccgcacag gccgtaaaag agaaaatatt gttcaccaac
    11821 acatttccat gcaccgtaaa tgccagaaaa tcaacataca ttccccggat cgttgcaaac
    11881 agccccagac cggaacctcc ctgctccatg gaattgatcc cctgataggt atccagcacc
    11941 tttccctgta atttcaacag aatctgcagg attacgtaat acaatgcggc tccggcaact
    12001 cccatgccaa ggtaacgaag tacatagaac gctttcgtct tccagccctt ctcttccata
    12061 aaaaagagca ggatcacata tacgcacagc agaatggcaa agggcagata tgcctgatag
    12121 atccccatac tgaacgcaag acataccgcc ccggccagcc agcctttttt ctgttttttc
    12181 gtaaaaagca ctgccagaat tgcgaggaac agtgccatca tatagccatc catggtaaac
    12241 acataggcaa aagtggatgc cagtgccgga aaagaaacca gcaatccgct cactgccatg
    12301 attaccaacg ggtctgcaag ttccagcagt tccgtcaatg ccaccgccgt cagcgccagc
    12361 cagagcagac cgatcagacc aatgatccac ggaatggtaa aatacgaaga aaagccgcat
    12421 gtcacagaca gaaaccaccg tccggaagtg atcatattct gatcaaaata catactgctc
    12481 aggccgtcat gattcggaat atccgaaagc atcaccggca gatggatcag aatccccagt
    12541 acaaatgctg agagaaaggc gatcttccat gtatttttta tttttgttct gctagattga
    12601 aaaaaatccc acggggtctt catccgtctg tcactcctta tttagtcttt ccgcgatcgt
    12661 ctgtgcgatc ttctctctgc cggcctcatt gggatgcagg ccgtcatcag tatagagata
    12721 cagatcatcc caggtgtcat gtggataata gtcatgatag atgtctatga cttctacatt
    12781 caaagcctca cacagcccga tttccgcatt gacataatcc tccagcacac caccgcccag
    12841 atcatattcc tcacaggtca gttccggatc cgtataccag gtatagggtg gggtgatgaa
    12901 gataatgcgc agctccggat aggcttcccg caactccttc acaatactgc ggatagctcc
    12961 acagtaggta tattcatcat acggatctgc cgtggattcg atgggagtgc ctgagtgata
    13021 atcattcagt ccggaaccga taaacaggat cttcacctgg tcaaaatcga tctgtcccag
    13081 atctcccaaa gtatcctcaa aataatccgt ggctgcttct cggatgtgca cggtcctctg
    13141 gacaccgaaa tccttcacca caaaggaacg taccagtccg gccgcagaaa tggaatcctt
    13201 ggtatatccc agccttctct ccgcatccgc tctgccaaga accgttcctc ccagcccacc
    13261 gttgtagcat tttaaacctg tcttctcctg aagcttcgct gcgatggatg tctcatcacg
    13321 gcacagacca tatacactgt ctcccagaaa cactacatca taaggaatcc tctgatattc
    13381 ccggtccccc ggaaaaaaca gtaccaaaag cagcacgaac aaaacagctg ctgtcacact
    13441 atataaaatt tttttcttcc agctgccgtt tttcttcata aacctcagat attatccttc
    13501 tattcttcta ttcccgccgt ctgcaggatc tctgcgatcc gggcagcccc ctgcccgtcg
    13561 atcagggttc tctccgcttc ataggttttc tgccgcaatt ccctgttctc acaggcattc
    13621 cggcaccagc tgccgatagc ttttcttgtc tcttccgggg cgtaatgcca ttttccggca
    13681 tatccggctt ttttctgctt tcccagccat tccgtcagcc cttcctggtt ctctgcgtaa
    13741 gaaaaacaga caaaaggcac tcccagaact gacaattcat agattgtcgt tccaccggca
    13801 gtcaccgcca gctgacaggc cgtcatcagt cccgccatgt ctttcacatc gcaatgcaga
    13861 accacgttgt tttgctccga tgcatattgc tccaattttt cccggttcgg attaaacctt
    13921 cccaccacca gatgatacct tatatttttt cccgccagac tgtgctcaca cagttcccgc
    13981 aggatttctc ccgcaatgtt gtctttgtct ccgccaccgg tggtgatcag tatatccgtt
    14041 acatcatcct gcaaaatata ctgtgctgcc gcctcacaga actgctgccg caccggcaca
    14101 taagcagctc cgagatactg tccggtactg cttccataca gttcccgata cttttcccgg
    14161 gaagcaaaca gattgtaatt gatcacaccg tccaccggaa atgcatgctg ctgcatatca
    14221 tccagcagat acaccctgcc aaaccggtgc aatgcctgca aataagcatc cgtcaccgca
    14281 tagctgtcca ccagaaaaac atgcccgctt ccccggatca gtccttccag cgccgaaagc
    14341 tctccttcca ggtcggcagg atccgtccca aggatcttca ccggcatgtt ccagctctgc
    14401 aacagttccg cagatgcctg ctccgcgcac aaaaagcaga gttcttccgg atgctctatt
    14461 cttttttgta attcctgtgc caccgtgagg caacgcatca gatgccctgc ccccgtaaca
    14521 gcactgccgt ccgcacgaaa ataatacatt tccgctcctt actgctgttc cttctctgtg
    14581 aaatccacaa gagaccagtc cataggggtt cccagcctgg catctctggt gatccgtttg
    14641 cccaacagtt cctcataata tctggtatgc agtccgcagg aaggtctcac agaccgcaga
    14701 ttctccggag taaacacatc cccggccttc atatcctgtg ccacgaacag ggaacgggaa
    14761 tgttctctgc tctttttctg cttcggtgtc agatcgtagg tcacctttcc tactgctttc
    14821 tcgatcatgc ggatgtgatc caccatctct ttgaattctt ccggttccat ggagaatgcc
    14881 gcatccgcac cgccgtctgc tctgcgcagg gtcagatgtt tctccaccac cttggctccc
    14941 agcgccacgc cggcaccggc cacggcacag cccatggtat gatcagaaag acctaccaca
    15001 caatcaaaga cttccttcat ggaaggaatc gtcttcagat taatatcctc atagggtgcc
    15061 ggataagcgg aggtacattt caacaggatc acattctcgt tgccctcttc cttacaggta
    15121 cgcagtgcca gctcgatatc cgccagatag gcaattccgg tagacatgat aatcggcttg
    15181 ccggtacggg cgatcttgcg gatgaaagga atgtcgttaa tctcgaagga tgccaccttg
    15241 tataccggta cctccatctc ttccagaaaa tcaatgctgg tcagatcaaa gggagaagaa
    15301 aagaatacca gtcccatctc ctctgcgatc tttttcagct ttggctgcca ctcccaggga
    15361 gtataggcgg tctcatacaa cttgtgcagt gtggttccgt cccagatggt tccctgtgtg
    15421 atctggaagc agggatcatc acagtccagt gtgatggtat ccggggtgta agtctgtatt
    15481 ttaatggcat ccgcaccggc atcctttgcc gcctgcatga tagccactgc tctgtcaaaa
    15541 tccatcagat gattcgcaga catctctgca attacaaaag tgggagaatc ttcactgatt
    15601 ttatgattcc cgatcatgat ctctttgttc ataatagtgc ctccttaagg aaatacaata
    15661 tgttcgtact tcttgctgtc ccggatttct ttccagtgtt ccgctgtcat gcgcatatat
    15721 gtcacgtcat aagcagctcc gtttttcacc acgtgggctt ttttctcttc cacgacttca
    15781 cagccgcaga tcttatggag ctggatgact ccctgattca gggtgaatac atcactgtat
    15841 accgcatttt tcccaagatc cagaaacgca tggtcataca tactcatctc cagcgccagt
    15901 gccgtctgga tgctgcgcag ttttttctcg cccacgtaat aggcccatcc cagatctcct
    15961 gtgggattcg tcagccctgt gtaattgatg acgccggctg ctgtgccgtc gatctcaatg
    16021 atccagtagc tcacatccgg atcctgctgc actttggcaa accatttctt ctgcccttcc
    16081 agagtcagct tcggatccgt attcatatag cgggtaatct ccggatccat gcgccagcgc
    16141 atgatctgtt ccaggtctcc ctctgtcact tttcttaatg taacactcat tcgcagcccg
    16201 ccttttccaa cagttcacgc attcctttta actcaatggc ttcgtctaag ggaatgctga
    16261 caccaagctc ttcctccaac tgtccgatga tcatgacatg cgccagggaa tcccactgct
    16321 ccacatcttc gatcatggta tcctcggtga tggttcccgc cggaaccttt aatacatctt
    16381 caatcagctt tatgatcttc tctttcatat ggttatcctc tgcttatcct cattttttct
    16441 aacatttacc actgtccggt cttccgtccg gcagccgtca cccagcccca ggtcaatcgt
    16501 ataaagtctc cttcagccag taggccctct ccggtctctc tgccagtgtg atctcatagc
    16561 aggtaccgcc ctcctgtgta ctttccgtcc tgtggtaacc cagcttttca tacagagctt
    16621 ccaccggctt attcttggct gtcgggatat atctgccgcg gagtccggta tatccttcct
    16681 cctgcatctg ctcctcgata cggtcgatca ccgcattctc gatattcctt cccatgacac
    16741 ggcagctcat gaccagatcc gtgatctccg gcagttctcc ggcagtatcc acgatggctg
    16801 ctgccacgat tccgttgtct ccaaacgcat ccgtcacctg atacagaaat actctctttc
    16861 cggcatcttc cacgatctgc tgcatctgct ctctggtgta gcgtctggtg gtcaggttga
    16921 actggttcgt tttattcaaa agctgtgtca gccggtccag atgttccttc ggatccaccg
    16981 ggatcagaca gatctccagc tttttcacat agtcttcaaa gctcaccgcc tgctctgcca
    17041 tggcatttct gccggcattg gcccggtact gtgcggtctt ctcccggtcc tctttcgtga
    17101 tcactgcccg ggcaaaatat tcttcataaa tctttgccat gcagggcgcc agttcctccg
    17161 gtcttgccgg gaaatcgggc acagtcacct ccggcagcat ctgccgtacc atttcccgtt
    17221 ccgcttcgct gtcatcaaaa aagacaaagg aatccgttcc cagattcagt tcttccgcca
    17281 tcttacggac attgtccggc ttcgggtccc agttgatcct gcgggctgcg aaatcttccg
    17341 gcccaagcaa catatgggga tgatgttcca agatctccat ggcatcctct tcattatttt
    17401 tggatgctat ggctaacagc acaccctgtt cctgcatcag ctttatgacc cgttgcagat
    17461 ttttataagc cagaccgctg tggtcttccg acaaaagcac cggtgtgtga tccgtctctc
    17521 cggccagacc gccccacagg gtattgtcca gatccaggac cagcaccttt ttaggggtgc
    17581 gttcctccag ttccgcctgc tgtaatattt tttccgccag aagagactgg gtctccatcc
    17641 ccagcaatac ttttcccata taccacattt tcagggaaaa cgccttctcc tcgccctgat
    17701 gctctatgat ccagcgataa ggaaatatcc gcgcattgga atgtttctcc gtcagctgct
    17761 gcagtgcagc actccacaaa ctctccagct gctgtttgcg ttccggatcc gccagcacag
    17821 ccagttccac cgcccacaga tatgcatcgg agacatagaa cacaccatgc tccggcagac
    17881 agccttccag cgtctgaaac cagtttccga tccgctcttt tgctgtctgc ggatcatagt
    17941 catgttccag cagttctgcc agatccatga tcagaaaggt aatgtccggc tgaaacgcat
    18001 gataagagga agccgggttc agcaaagctc ccagttcatt tccatagcct tccgcatcgt
    18061 agacttccgc ctgtttctgt aacatacgga tcaccatatt catatttaca ttggacaata
    18121 ctgccagtct gcctgttgcc atagtctcag attcctccgg gattattttt cctccgtcat
    18181 cagacggtat ttcatctctg cgatcttcca gtccgtcaga ttatcgatat cctgcacttc
    18241 cagttccgat acgatcaggg gcagaatatt tcccaccatc agcttcttat tcactgcaaa
    18301 gcagtccgta cggaagacat agaactgtcc cacatcatga taatggggct gcaggtcctg
    18361 ggatctgctg tccagatatt cgggatattt aaataccaac cgttcctgct ccactaccat
    18421 ggcccgctgg ggcgggtagg aaaagctgac caccggaatc agggtgtccg catcgcttgc
    18481 ttccagctgt tctaccgctg ctttcagttt ttccgctgtc acaaaaggcg ctgtgggata
    18541 gatgcagcat gccacatcaa aatgcatgtc acgcttctca tattccgcca acacttccgc
    18601 cagcacatca ttggtggtgg caaagtctcc ggaagtcgcc tcactgcgga aaaacggcac
    18661 ctctgcaccg tactttttcg caatctccgc aatctccgta tcgtccgtgg ataccatcac
    18721 atggtcaaaa agtctgctgg acaatgccgc ctcgatggaa tatgccagaa tcggttttcc
    18781 acaaaaatct ctgatatttt tcttcgggat ccgcttgctc ccacctctgg cggtgataat
    18841 cgctaatctg ctcattttcc taacctcatc tttctcatgc cccatggccg cccttcaggc
    18901 ccgtagcttc catacaaact gccggatcct gtaatagaga tagaaccgca gatcgctctt
    18961 tttctgcatg gcgatgaatt tgcgttcctc cggcccggta tattccagat aatatttgtt
    19021 cctgtcgaac tccggaaaat gtcgttctgt cccctcccgt agttctttta caaaagacag
    19081 cttcggcttc tccactcccg acagatagga gaataaagtg atcacataat acagctccgt
    19141 aaaacggtat tcgatctgct cccggtattc cgcaaggaac cctctgttct cgcattccat
    19201 gtacaaaagt tctgctgctt tcatacgatc acggcatttt tcctccgtga tgtgatgtac
    19261 cgtggacacc gcatgctggt aataataata cagcggttcc tccactctct caaaacgtcg
    19321 aaagtacagg ctccacaagg ggcctgcaca gttgtcttcg tagaaaatat gctccggaaa
    19381 atccagatga ttctcccgga tcacactatg tttgtaaatt ttcagcacca tactgccggg
    19441 acgcatgaac agtctctcat gcttttcctt cgtcagatcc ccggtctgat ccggtgtatt
    19501 gttgacgatg accttcccga cctcaaaggt atgatgatcc accagactgt aatcgcagcc
    19561 caccagatcc gctccggttg cttccgcttt tttcagaagc ttctcgtaat agtccggtga
    19621 cacccagtca tcactgtcga tgaaaccgat ccattccccc tgcgccaccc gcagtccggc
    19681 atttttggca ccgccctgtc ttaagttcac agggttcgga atgacacaga ccagctccgg
    19741 ataacgcttc tgataatcca gcaggatctc cagagaatcg tctgtagaag catcatccac
    19801 gcagaggatc tcataatcgt acagtccccg gatggtctgt cccaccaggc tgtccagaca
    19861 ataattcagt tttccctccg tcgccatatt atagacgggc acgatgatgc tcagtttttt
    19921 actcataggc gttacagatt tccccgttct atttttgctc tgccgtcttc tacccgatag
    19981 acaatgtcac atttttcaat ggtctgcaat ctgtgggcga taatgatcaa ggtcttgcgg
    20041 ccgtgcagtc ggttgataga ctccatgatg gctgcttccg tatcattatc cagtgcggaa
    20101 gtggcctcat ccaggatcag tacctccgga tcattataca gcgctctggc gataccgata
    20161 cgctgtctct gtcctccgga gaggcggatc ccccgctcgc cgatgccagt ctccagacct
    20221 tccggcagag tcttgataaa ttcatccagc tgtgcctctt tcaatacttc ccacagtctg
    20281 tcctcatcaa tcttgtcctc cggcacaccg aaggctacat ttttacggat ggtgtcatcc
    20341 agcatgaaga tcatctgggg gatgtagccg atattcttca gccatttccg gtaattgccc
    20401 ggttccttta catccacgct gtctgcatag atatgtcccg tactgggctc cagcagtccc
    20461 aacagaatat ccaccacggt actcttgccc gcgccggaag tacccacgat acctaccgat
    20521 gcacccaccg ggatcttcag atccgcatgg tcgaagatca gacgctccgt attgggatag
    20581 gcataagtaa tatctttcat ctcgatgctt tcttttacat ccagcttttc atcttcatcg
    20641 gtagcaaagg acatgtcgat attctgtccg ttgatctcat cctgtaaatt atcgctgaca
    20701 cccatgaaga aaggttcaaa ataggcgatg gaattgatct ggttgttgat acggttcacg
    20761 cagggcaaca gtaccagtgc cgctgccgcc agagttccga acactgtcag catattttct
    20821 gtaggcacac cggtggccac cagaaagatc atgtatccta ccatagtggc aacgcaggct
    20881 gtctcgatca gcagcttcgg cacctgattg tacaggctgt aacgctgtac ggcatccacg
    20941 tagccttttc cgcatttgcg gtactcggat acgaaatact gctccttgcc ggaaatcttt
    21001 acttccttga taccctgtac cgtctgggag atccatttga acagactgct gtaatagtcc
    21061 tgattgtcct taccggcctt gtacatgatg ggcttcagca ctctcttgat cagccacatc
    21121 aacagaagca ataccaccgc catcaggatc gtcatggtac cgctggaaat aaagcagtaa
    21181 ctgatgacaa acagggaaac cacgctgtcg gataacagct gtaacagtgc caggatcagt
    21241 gcatacatat tgttgacatc ggaagtgata ctgcgctgca ccacagcggt atccgcattc
    21301 agataaaatt cataacctct gcgcaggtaa ttacgcatca tgcgctcgga agtgcggaac
    21361 tggttcgtat agacaaagga taaagtcagc ttctgctgta cataagtaaa caggttcttc
    21421 accacaaata ccagaatcag cagcaccatg acaataatgg agaacgtacg atagctctcc
    21481 acacccatga tctcatagca ggcttccgcc acccggctgt gttgtaccgc ttcgggatca
    21541 atgacgatct gcatgacctc caccagcatt cccacaccgg cagtctgcaa aaaggcactg
    21601 atcaccatga ggataatgag tcccaccatg gttcttttct gttttttgtc cagcaggaca
    21661 cgtaatttct tgatgatctg tatcattgac ggtctccaaa cttatatctg ttcattctgc
    21721 ctgtggatca gtggatcctc ataggcatcc ataaaatcag gtccgaggcg taccgcttca
    21781 tccgcaaaag gaagtccctt tacctccgta aatatggtta ccaccttttt aaaacgcaga
    21841 ttcgggaaaa agttcagcat ctccatgggc atggatgcat cgaactgggg atattccgca
    21901 aaaagcgtct ggtaatccag caggtctctg ggatggggct ttaaaaatat ggtgccctct
    21961 ttttcataca tcttgataat atcccggaag atctgttctc tcacatccag tgcacatacc
    22021 ggatccgtga ggatcaatat tttatctccc accttattgc tctcttcgat ctggtgttcc
    22081 agttcgtctt tatttctcac gaatgcctgc aggagcagtt gcttatcctc cgctgtcagc
    22141 cggtcttcca gcggctttct gggaagttcg atataccggg gacagggata gcggatggcg
    22201 gaaatgtcat ttacttccat atccatgcag tattttccgt agccgttctg aacaaagatc
    22261 aggttcagat acatggacag gaatgccttg atcttaaaat gtccacggtt gtcatatcgc
    22321 gccgcgtcaa agttcttgag acagttcagg ccgtcttcca cggcatgata gcggatatgg
    22381 ttctgattca ggtagtagcc gatgggatcg gaatcgcagt agacataaat gtccttgtaa
    22441 gtccgaagat ctaccggcac ataagaagcc tcgagtctgg cgtactcctt cgtaaagcgg
    22501 atccggttct tcagattccc cagaaagctt ccggtgtcct cccggtattt ttccagttcc
    22561 gggaagaaat cttcacgctt ctcatcaaat tccagtacct cttcgaacag tcccgtgctc
    22621 tccacccggg atttcaggtt ctcgaaatta ttggacattt tggacagcac taaagtagca
    22681 gcccctgctt tcttcgcagg atctgcctcc gcccttaatt tcagttcttt taaaaaagtc
    22741 acatatacat gatagtatgt gtgacagata tagattctgt ctttcatata taatctctat
    22801 tattgcttat ttccatatag ttttgtaact attcactccc catccgcaaa atcgctgcta
    22861 tgcggattct atgaatagtt acactatagc acacttttct cttccagtaa aaaatcttat
    22921 gaaattttaa gaaagttggg gtaacagttc agccatagga tgacagaatt ggtgcctggg
    22981 tgcttgcaca caaggcaacg attctgtcat tctatgagca gaacgcaagg tcaatgaaat
    23041 aaaagatgag ctgcgatgcg gcgccggctg cgaagcagac gcttttatga attggccttg
    23101 cgctgaactg ttgcaacggc tgaaccttta caatctatac acccagatcc cccagtaaga
    23161 gtcttctgtt tcaaagtatc ccacaagttc catgcccatc ctgtcggcat cgccgatctc
    23221 agctccggag aacaggtact cacatcccag ttcccgcaat gcctccatgt caaattccag
    23281 gccttcatag accagtgagt ttcctttttg caggcgcatg taattgccgg taatactgtt
    23341 gaacagatag cagcggttcc cccacgtatc aaagtatacc cgcacttctt cgtttttctc
    23401 gatctccggt gcaatgactt cccggaatct gtgcttgtac tccaggggat aattgttgga
    23461 atatccgtcc accgtataaa aaccatgcat cagtgccggt gccggactga tccccagatg
    23521 cgccacacga taggtgctct tgtcccggcc aatcgcatca tcgatctcct gcatcagatc
    23581 ctctgaaaac cagctttccc aggagatata tcccgtgatt ccggatccgt tattgatctg
    23641 gttcacattg agatacattc cggaattcac tttcaacagc tgaagcgtcg gcaacagaca
    23701 cactgccagg atcaccagtt ttcccggcag catcctgctg tcggtatgcg gcactttcgt
    23761 tctccacaat accgctgccg ccagtgcgaa ctccagatac cagtccgcag gatacagcca
    23821 atagaaccgg tggatctgaa aatagtgtaa aaatcccgtg acgctgtttt tgaaatcaac
    23881 gaccaccgtc atatgacaaa atgcataaaa caatgcgatg ccgatcagga ataaaaagtt
    23941 gataacagcc gccttgtata tcattctgtc cgtttcttcc tttttgcaaa atgctcccag
    24001 aagcaggaat atgacaatcg gcaggatcag atatttatgt aaggacggtg cgtgttgtgc
    24061 gctgttccgg aagaggctcc aaaaagtctc aaaaaaggga gtcgcggaag acaccatttc
    24121 ttcccggtgg ctgacataac tgctgtctcc caccagaatc tccaggatca gtgaccagtt
    24181 gacaatgaca taagtcacca gcaattctgc aaatcccaga accggccatt tattctttct
    24241 tttctgaaag aaggcaacaa ggagtgccaa aagccacaat cccagcacca cataaccggt
    24301 atacaccagg tggctggtca ggccgaacag taacgtacac atcaatgcgg gaaggattct
    24361 tttccgtttc cacaggcaca gaaaggcata tgccaccaaa ggaatgccga attccgaaag
    24421 accgtagacc ggatataagg gcagcacaca gaaacatgcc ccggctatca ccgccagaat
    24481 actgctctcc gtgctctcct ttaccaacag atacattccc agaaacgcca gcacaaaaca
    24541 gatcatgtat tgtgtaagaa acgccaccct ggcagaaaac agacaataca gcggcaggaa
    24601 tagaatggcc gacggctgca taccgctggc attgacccct cccagcatct ccggatatac
    24661 cgtactgccg tcccataaat gtctggcggg aagcatataa ttcataatgc tttcatccag
    24721 ctggtcgttg atctcaaaga tgcagccatc ccccagaata aaataggggg aaaaaaatac
    24781 aaccacgatc aaaattccgg tccaccagag aggaatcctg tccagccaca ggaggaaccg
    24841 gttgatctgt ctgcctattg caggtttttc atcagaagtg aacaataata tagtccccct
    24901 tatctacaat atacccctcc tgcggatatt ccggcagatc cagagactct tcttccgctg
    24961 ccgcatagag gctgatgtcc tccagagcca ccagctgatg cccgtgcatt tcaaaaaatt
    25021 tcaccagctc cgcatcggaa tcaaaggcat agcgtcccca gtcgataaca gacaatgccg
    25081 gtgtctgtgc cacccacact cctctggatc cacgattgta tttatccagc agatccgggt
    25141 ccaccagatc cgccaatcgt ttcatcttat aataaacagg gctgctgtag ctcacatagg
    25201 catcctgaac tatgctgtag ggaatatcat aatttcccgt gaagatcacc ggttttgaag
    25261 tatcaaagtc ccgttccaga tccattgcaa tctgatccac ggtcttcact gccgcatcat
    25321 attttttctt gtcaatatag aaccatctgg tcatatccat acactggttc cagaggatca
    25381 ccgccagcac cagaactgca agtgccctca caccagcaga gatttttctg ccagccgtat
    25441 tcttcgcttt tggggacata gttcccgtca gctgccagat cccatacaca aaaagcagcg
    25501 ctccgtatcc acagaagacc ggcaggaact gtgccgaacg gtacaatgtg gcttttcctt
    25561 caatgaacag aagagaaaat gctgccaaat aagcggcagg caacaacaga agtgcccaca
    25621 gatctctgcc gcggatcacc cgcaccagcg tcaccacaag gatcacagcc gctgataata
    25681 taaaaatccg gatcggcaga taagcatagg caaatacccc atacagcaca aaggtcctct
    25741 tgaggatcat ggcaagctcc ccgaaagccc ccggctgcag catccagccc agcatttccg
    25801 tcacggaacg ctgtaccgcc tctccccgca gatattccag gtgaaatgct ttcgtcacta
    25861 ccacgatcat cacactacgc agcacaatgg ccacaagcgc cgccaccgca ccagccacca
    25921 gtgtggcaaa tatgtccttc tcctgacgtc cccgtgcgat ccgctccgtc agcagcaaga
    25981 gcatcagccc cgccagccac aggatcataa aggactcgta acatcccatg gcgatccaca
    26041 aaaagactgc cgccgtcagg atcttagcca ctgccaggca tcccagtttc tgccggatac
    26101 cggatccttt ttgcattttc cgggtactgc tctgccattc cctgacacag cacaggctga
    26161 tgccacagca tagatagcca atggcgatac cgttatgcag gaaataagta aagacttcac
    26221 tgatcagggg actggagaga aagacagcac cgaagaaagc atagcctgcc gtcggaacct
    26281 tctctcccaa tacagaataa aacaatgcag accagacgac cgctgccagc accagtatca
    26341 caactgccgc aaaatccgtc acgaagggaa caaactctgc cagcgatacc accttattca
    26401 gcaaaaacag aacccaccgc cccacaatgg cgatcagtcc ttcttcaaaa taatataagg
    26461 atggcgtatc atcgatgccg atggttgcat ttgtcacctt atagccgtag gcacacaccg
    26521 ctgtcagtaa caggatcccc atatacaggc ggttctgctg gaagtgtttc ccgtagctct
    26581 tccatttctg catcactttt tccatgcttt tcatccattc ctttttctta ttcgtccatt
    26641 actgtgctac tcttcctcca gcttctccag tcgaaagatt gcaaaatcat aatacatata
    26701 atgaaattcc aatacctgtt catagctgta ctcatcttcc atttcattgg ggtagccccc
    26761 ctggaagcag atataccaca gattatcata agtgtccagc tgactgtaac tgctcagtgc
    26821 ttccgtattc acaaaaggaa gactgtacag cttatatgcg atcgtgtagt aatgcagttc
    26881 aggatgatac acattcaaaa agatcgtatg cgtatacggt ccgatgatcg catcattctc
    26941 cgtcatgtat tcctccacga aattctcgta ggtttccaga ccgtcatcat attccagcct
    27001 gatctcggag gtatactgta acaggaagca gagacctgcc gttcccagaa ggcctgcagc
    27061 ccatattttc ctgcttttgc cttttgtgtt ctccgtgatc tgtgcaaatc ccacggcata
    27121 ccacagcatg acaaagccca tgcccgggaa tgcataacgg cccataaaac agttattgac
    27181 cgtagcggag atcacgcctc ccacgatacc cgtcagggca aatgtgccaa acgcgatggc
    27241 cggtgcaatg ttatgattct gtctcaccca gtccacggct ccgtaactga gtaccagaca
    27301 taatgccatt cccaacagta cgacaatgcc gatgggagtc tccaccgctg agaaccattc
    27361 cttacaataa tccatcacgg aatacagcgt tgccagttcc gcagtggaac cgtcatcgta
    27421 gcgcatccgc aggacaaatt gccggatcgt caccgccagc cagggagaaa acaccagcgc
    27481 caccgtaaat ccggagataa aatacccctt gatcagctcc ttcttatggc agatcaggat
    27541 cactgcgaaa aacagcagat agaataagaa cgtctggatc aatgcaaagg tatgacaata
    27601 taccgtgccg atactggcga tgcagaatac gatccatttt ttccgggccg gctctctgaa
    27661 aatgtcatag gcagacagtc ccgtcaacgt cagaaagaac agggccaccg catacattct
    27721 cacatttccc gcctgtacac tgaagatcgg catcagcagg gaaaacagca taaaatacac
    27781 cgaaatctta cgatcaaaca attttgccac ataatatttt cccagcagca tatatcccag
    27841 catgaacagc acggacatca gcttcagtgc ccagaaatgg gttcctccgc cgcacagatg
    27901 atagaagatt tttaacaaca catagtaaaa cggtgagtga tcatccgtgg ctgtaatata
    27961 gatcagccgc ttccagggca gggataccat ggatgccgaa taagcctcat cataccagag
    28021 gctgttggta atgcagaagg ctccccagag aagcagtgcc gccaccggca gtccatgcca
    28081 cagcagtgcc cacatattgt tctctgtgaa attatgctgt tttcttctct gtgtctccat
    28141 tacgtccata gtttcttccc gttctgtctt tttttatttc acatatcaga tttcagatct
    28201 tttatcagta tctccggtac agcaccatat ccgcatccct gccgagctct tcataccctg
    28261 ccgtccggca catttcatcg atggctccac cgctcaccgt tgccagataa cgactctgca
    28321 gctctgtcag atgctccgta atatattccc gttcacagca gctgatgcca aagcccttcg
    28381 gtgcactgta caaaagctgc catttcagat tctgatggcc ctccggggtc tcatctccga
    28441 aggtccagat cactacattc tcataattcg gcacctgttc tgtgtccatg gccagttttt
    28501 ccgagaagat ttcccgccag tactccacct gttcctgccg ttcctctgtc acatagggaa
    28561 tctgatagtc ataaggctcc actgctttga agctgtacag atacaggaaa gccgctccca
    28621 gaagcaccgc tttcttataa tatctggtct ccagcatact gacgatgaaa atgcctgctg
    28681 cgataaaggt caggaaatgt ttgctgcctt ccaccatttt gtacatgagc aaatgtgcga
    28741 acagcatggc gatgaggctc agggcaaaat gcacttcaat aaccagctgg ttgtgcagtt
    28801 cccgttttct gtcctctgtc agtgtgtacg gtccgaacaa cgcctcccgt acattcttat
    28861 cctccggctc cattgcgatc cgttccaccc gcttcgcccg gcgcagtgtg cggatgtcca
    28921 gaaaactctg caccaacagg atcagcagca ccaacagata accgtcaaaa aaggctccgg
    28981 aagccaggcc attccgtccc ccttcgatac aatgtctgta gaattccagt cctttccagt
    29041 aaagtgttcc gaaaaggttg cggaagcccg cgccaatccc atcagtgaaa aaggtagtga
    29101 tccagtccgt ataaaacagc ggtgtaaaat attccgctcc cagataatgt ttgatcaggg
    29161 cataggtaat tcccgttgcc gccactatca gaatcgagcc gatccaccct gcctttttat
    29221 tacggcagat ccagaaatag caggccagag ccagaaacag cagcatataa ggccgcatca
    29281 gagtcagcag taccgagatc acgagcatgg cgatcagttt cccgcggctc tcctttttca
    29341 gatagctcat ggccagtccg tagaacagga tcagcaagct gaagcagatc acctccggca
    29401 taccggacag catatatctg acaaacagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29521 nnnnnnnngg aagatacacc tgtccgatgc ttcttccttc cgctgccgtc cgtaccagac
    29581 agcagagcag gggaagcagt gccatcagta ccgctttcag cagtgtcata taacgtttgt
    29641 cctgtttcca ccaatccaca aaacgttctt tcttcatccg ttttcctgtt acccttctgt
    29701 tttatttcga tttaccggtc ttcctcaccc agatggatct tttcccggat cacatactgg
    29761 ggagaattat tcatgctgat gtaaatacgt cccacatatt ccccgatcag tcccagcatg
    29821 atcatgatca tgccgccgaa aaataccacg gctgccatca gggcactaaa gcccatgggc
    29881 acattaggat tcaccagacg cttgataatg gtatagattc catagaggaa gcccataccg
    29941 gcacatccca cacccagtgt agtggcaatc cgcagcggtt tcaccgagaa tgcggtaaat
    30001 ccgttgaacc ataagcccag cagctttttc aatgtatatc cggaactgcc ttcctctctt
    30061 tccctgtgac acaccgccac attggtaatg tttttggtgg cccggagcac cagtccaatg
    30121 acataggggt aggaattctc atagcggatc atatcctcca ccacgaatct cttcactgaa
    30181 aaatagctgg aaatatacag atccgccggt ttatccagca tcatccgggt catcagctca
    30241 ttgaccttgc ttcccatatt ccggaatgcg gaatgctgtt tatgttcgta tctggcgtaa
    30301 acggcatcga aaccctcttc cagcttgccc agcagcttgt ccacttcatc cgccggtgtc
    30361 tgtccgtcat catccagaca caccacgtaa tcgccgtcgg aatgacgaag tcccgccatc
    30421 agtgcggcat gctgtccgaa attccgggca aagccgattc ccttgatatt gtcatattta
    30481 tcacacagat cccttatggt tcccatggta ttgtccgggg aagcatcgtt caccagaaaa
    30541 atatcgtatt catactgcgt gagttcctgc attttttccc ggatctcctt caccacatgg
    30601 ggtaaggtat gttccgagcg ataacagggg attacaaaac ttactttttt cataattgtg
    30661 ttctattcct cgttttcctg atcatttccg gaattcactg ccgccatcca gacgatgtcc
    30721 cggtagtcgc cttcaataca cacatcgtcc cgcagcaggc cctctctgcg aaatcccgct
    30781 ttttcatagc tgcggaccgc ctgcccgttc tccgacagag cccgcagata cactctgtgc
    30841 agctgttctt cctcgaagca gtaccggagc atcagctttg ccgctgccgt accgacacct
    30901 ctgcctctgg catcggcctc tccgatgaaa ataccgtatt ccgccttgtt atgatgtctg
    30961 tcaatatccc ggatatagac ggatcccagt ggcttgtcgt ttgccagatc acagatgatc
    31021 atctgcacca catcgccggt ctccacatgg gtccggatcc agttctcatg ggactctctg
    31081 gtaaaaagag cctgatagat aaaatttttc cgcacggcat cactgttgcg ccatgccacg
    31141 atccggtcgg tatcctcacg ggtcatcagc cgtaaataga ttcctgcccc ggtatcccgg
    31201 taaactccgg gaatccgttc cggcgctatc cgctttttta tttctgtccg catggtatcc
    31261 tgcataatat tcactcctta tgcctgtctg taggcattgc acagatcgat cactctggcg
    31321 atctcatcct cccgcatctc cgggaacagc ggcagggtca cgcagtgtgc ggccagatat
    31381 tccgcattgg gacagtctcc cggctgatag cccagattgg cataagcctt ctgtaaatgg
    31441 cagggtaccg gataatgcat gttacattcc acatcatttt ctgccatata ccggataaag
    31501 tcctcatgat ccggcagcgt gatgacaaac agatggaaaa ccggagtggt gttctccgga
    31561 tgtctctgca tggtgatcaa cggatttgta atctccttca gataacgatg tccgatctcc
    31621 tgccgtctct tcgtccactc cggcagatat ttcaggctga cactcaacac tgccccctgc
    31681 agaccttcca gccggtagtt gtaaccgatc tcatcatgat aataccgcac atcacagcct
    31741 tgattcttca gacggtcgat atgcttataa tatttttctt cctgacaggt aacactgccg
    31801 ccctcaccaa aggcgtagag gttcttgccc gggtagaagc tgaagcaggc caggtctccc
    31861 agcgttccca catttctgtc ctcaaacttt gctccgtgag cctgggcaca atcctccacc
    31921 acaaacagtc cgtgacggtc tgcgatcttg cggactccct catagtcaaa aggctgaccg
    31981 tagaggtgaa caccgataat gcctttcgtc ttttccgtga tcttagcctc cagtacggaa
    32041 ggatcgatct cccaggtatc cggcgtacag tctgcgaaca ccggtgtggc tcctgtatag
    32101 cttacccccc atgcagttgc tatataagta ttggccggaa cgatcacttc atccccgggg
    32161 cctacgccca atgccagcat tgccagatgc aaagcggagg taccgttgtt gacaccactg
    32221 gcaaatttgc ttcccacata ggcggcaaac tctttgtcaa aggcatctgc atattttccg
    32281 ccggaaaaag cggtctcttt acatacgttc tcaatggctt tccggtaggc ttccgcatgc
    32341 gcctccacat ctcttgtcag atcgattctt ttaatatgtg ccatggtatt atccttcttt
    32401 cttccgtttc cggggcaaaa gccctcaacc ttgatgccta cggattgttc cgtgctacgc
    32461 acttatgagc gagttcatgt gggcttagac ctttttcccc tggcatctta tatatgcttc
    32521 ttaatataaa actcgttaaa caatcttgca atctgtacca gataattaca gatcgtctta
    32581 aataccttaa cggtagtatt gtaatcttcc tgccactcca ccgggtaatc caggatcttc
    32641 acaccccgct tctccgcccg taacaggaat tcgatcatat agaaccagcc gttatcctga
    32701 ctgcagccct caatcaggga aagagcagtc tgtctgcgta caaaggtaaa gccgcagatg
    32761 gcatccgtgg acttcatccg caggaacagt ttcaacagaa tcaacagtcc ctgggacgtg
    32821 atcctgcgat accattttct tccctttgta tcggattctc tggcgaaacg gctgccgtta
    32881 atgtattcga tctgaggctg tgtttcaaaa agatggattg cctctcccag atgtttgata
    32941 ttggtagaca ggtcaatgtc catatatccc accacgtcac cgtggctcaa ttccacacct
    33001 ctgcggaaag ccgcaccgac accgcggaca ttgattctct catatcttac tgtttcatat
    33061 ttctgacaga gcatctccgc gatctgcggg gtctcgtcct cggaaccgtt atccacaata
    33121 atgatctcat aatcttcaaa tgcgatctta cgcagatact ccaccgtccg tgtcacaccg
    33181 ctttccagcc gcagacgctc atttaatacc ggaaaaatta tcgtaaacat cattttgtca
    33241 gtttttccac tccttctatt acataacgct gtctcttaaa gctcatattc agaatgacgg
    33301 acaggtattt gaagatcaat gtcagcatca ccgacatcat gaaaaatcca aaagaaatca
    33361 gagtcatcag gctcatccag ccttccaccg ggcgtaccgt gctgaagatg gaataaacga
    33421 tccacccgaa catgatcacc atgactgcaa acagaatact actgatcagc atggaaaatt
    33481 tctccagtac atccgtgaaa atgatcagcg tatccagagc cagactgctg cgggtattcc
    33541 gctcttcccg gttcctggtt ccctttgcca tctctttatt gtcatagact atggtctccg
    33601 cccgcagtcc gcagttcata tacatggcct tgcggtaagg aatatacgca ttcatctggt
    33661 tcacacggtt cactgcccgt ctggaaataa tgcggaaacg ttcctgacgc agcttatggg
    33721 tattacggct tcccaaattg tacaccgcat agaaaagtct ggaggtaaat gctacatccc
    33781 gcttcggtgc cgcagccacc acgtcatagc cttccaatgc cttatgatag atatccatga
    33841 tcagggacgg ttcaaaatcc agcagacaac ggtcgaattc aaagagaaaa tctccaacag
    33901 ccagatctct tccggcgttc atggccattt ccaccccctg ataaaagctc agattgatca
    33961 ggctcaccac atggcgggta ttgctttctt ccagataccg gtggatctcc tcaatggtat
    34021 catccatgca gcagtcattc acacagacga tctcatattt ttcaaaattc tgttccatgg
    34081 ccccgcagac agtcttcagg aactctctga tattttttcc atcgttgtgc agatacagca
    34141 cgcaggatac gaaatttttc tctttattct gtattgcttc tttctccata atgtttcctt
    34201 tttcttcgcg attaatctaa aaacggatcc agatcatata cggtattgat cttgcccgat
    34261 aatacactga taaaaacagg atcttcggaa agagcccgga ttactgtggg tctcgaatcg
    34321 tcgatctccg aagccttcag aatgggcttc atggaaaaca aagatttctc atagctgaac
    34381 gcatactctt cccgcatata tttccgtgcc agtgtggaca tatactccca ggcgctctcc
    34441 ggtctgccgg ggcaatcgtt acagttcccc agaaaacctc cctgacagca gttgccattc
    34501 tctcccacac agggaaactg tggtgtcgca tcatagctgg tcttatgagg agtaaaggtt
    34561 accgaagtca gggaatgata accggtcttt ccaaacggca taatggagaa aaaggggcca
    34621 tccattaccg taaaaccgat attccggatc ttatcgctgg ctttgcagag aatgatctcg
    34681 cacagttcat atttcagtcc gaaatcctga gtctctgcgc cctttacttt cctcaggatc
    34741 tgattcacag aagcataggt ggcattcagg acaaagggag cccggtagac ctcttgtttt
    34801 ccttcgtgaa gtgccgtcac ctcataacag tccgtctgct tttcgatctc cgtgatcttc
    34861 cgtccgaaat gcagctttat gccgggatat tttgcaatct cctccatgag gtaatcccgc
    34921 aaaatatggg catcgtacgt atattcctcc gtcaggaacg ctccgtcaca caagcccggc
    34981 tgaaaatact cttccaccgg aagaggtttg caggggatcc ccgcatcctt gcagaatttg
    35041 cggaactcag ttgcgtctgt ccaggaaaaa tggctggaag tcgcgtagat ctgctcaaac
    35101 ttcgtatgaa tacagaagga atagtcctcc acaaaacgct tgaaataccc cgctgatttc
    35161 atagcggtgg acagagatct gggataatgg tagcccatgt gaactctggc ctgattgata
    35221 tatgtggctc tggtaaaagg agcctgttca atctccagta cctctacctg ttgtcctttt
    35281 tttccacagt acagggccgc gtacaagcca tacagtcccg caccgatgat cagtttatcc
    35341 atttttcctc ctgccggtga caagtgccag tacataaggc agcgccagca tcaccggata
    35401 tacatatcgc atctgtacgg tgggcccgaa caacatggtc ccgtaatagc ctgccacaat
    35461 ggcaaggggc agacagaacc gcttcctgtc tacgatcacc agcactgctg ccagggccag
    35521 atacagccac agatagcttc ccggcacgaa gagatatttc aataccggag tccgcagata
    35581 actgttgttc tccgcccagc tctccagctg ttcaaagagc cacggccatt tgctgtcctt
    35641 gtagatccct ctgtcattca gcgtgtcttc ttcccatctg gtctgcacat agcttaatcc
    35701 tgctctgccc tccacacggt tcacatccgc atgggtcgta tcaaacggag acaggaaccc
    35761 ggcattggta gccagtgccg cattgatcat atctcccgga tactgcgtca gcaggtgcag
    35821 atatacccgg ataaattcac cggaacggta gcgtgccaca taagtgttgg tatgtctttt
    35881 gacaggatcc gcaattccgg gatcataatc ccgatacgcc tcatccagaa taaaatcatt
    35941 gatcagtgct ttgtccgccg catccatggt attgtcatcc tccgcaagga cgctgactcc
    36001 cccgtgatag atcatacagc gggataactg ctggatcggc atgctcagca tctcccggcg
    36061 gtctccctgt tccgcatgcg tgacggaaaa taccgcagac agcacaaaaa gccctacgca
    36121 gaaagctgct ccgcatacca ccagcagtct cccccagagc ttcctcgctt tttttccgaa
    36181 acaaaaggtc aggaatgtaa aagccaacaa tacgatcatt gcatattttc cgttgttacg
    36241 gaataagatc atccccacgg ttcccagggc aaacagcaga tcccggatcc cgggccgcca
    36301 ggcattgcgg tcttcccaca gcagatctgc gagactgacc agctgcaaca gcaaaaatac
    36361 actgaatacc gtgtccttgg tcatgctgac actcatatac cagtgaaacg ggaaaaacat
    36421 ccccagtaac tgaatggcca gctgccaccc tgcggcagtt ttccggtgat gcagtaccag
    36481 cattccataa gccatgcttc ccgaaagcag cagcatctgt actgccgtat agaatgccat
    36541 tccggcattc acactgccga agatatgact tcccagccac agcatcccct gtagaaacag
    36601 agtatgcaca atggggtgat gatcgaaata ataatgttcc atgatctgtc ccgtctgcac
    36661 cggtgcatca taggcacaga ttccgggata atatgccaga tataccggca gccaggccaa
    36721 cagcagaaac aacagacttc cgaaaaatat ctgcacaccg gagcggcgct gcagccaggc
    36781 ggaaaggcgg gcataccatg cagcctgctt tttcccggtg ccttcggcgg tctgtgcccg
    36841 gttccaaagt cgatgcacca cccaggttcc aagaaatccc agaaggcacc ccagcactac
    36901 cgtccacagc aaagtacttc cgatgtagtc tgccgtccac aggatgtttc ccgcagatgc
    36961 cagatctttt tctgtttttg caaaaaatac aaacagacat cccagaacac tcagcaatac
    37021 aacgcctttg cgtctgggaa gaattttctt ttcctgcata ttacaaccct ttacgcacct
    37081 gatgctcccc atggacgcga ttcaggcgat attcaaaatc tttccggtcc ttggccgcca
    37141 tgacttccag catcataccg gcaaaaaacg actggatccc cgccagcagg ataaagccac
    37201 aggcgatcag tgtcgggaat ctgggcacca gaccggtatt cagatattcc aggaagaccg
    37261 gtacgaacag gatcacggaa acagcgatca ataacagggc cagcataccg aaaaagtgca
    37321 gcggcttgta atttttgtac agctgcatca ttttacggat cacacggaag ccatcgctgt
    37381 aggtattcag cttggactca ctgccctggg gacgatcgcg gtattccacc actacgtttt
    37441 ccacctgcat gttgtagttc accgcgtgga tcgtcatctc tgtctcgatc tcgaatccct
    37501 tggaaaatac cgggaacgtc tttacaaatt catagctgaa agcgcgataa ccggtcatga
    37561 tatccttaat gttggcatga aaaaggctgt tgatccccgc ccgcatcaga ctgttgccga
    37621 aattgtggaa cgggcgcttg ttctccgtaa aataagtaga ggaaagccgg tctcccacca
    37681 ccatatccgc ctggtgctgc aataccctgt ctaccatctc ctgtgcgcaa tccagcggat
    37741 atgtgtcatc tccgtcgatc atcaggtaac attccgcatc gatctcacgg aacatccggc
    37801 gtattacatt tccctttccc tgtgcatatt catagcgtac gattgctcct gcctccgtag
    37861 ccagctccac agtgcggtcg gtggaattgt tatcatatac ataggtcact gcctggggca
    37921 ggaacttttt cacatccgac accaccttgg cgatggtctt ttcctcgtta tagcagggaa
    37981 tcaataccgc aattttatcc attttccatt cctcgtattt cattttatca ttcagagcat
    38041 cacataaatt tgcaaaaccg cctgataaat caggctttcc ttttgcatat ataaaactat
    38101 tgtaccacgc ttattgattt acagccactg tttttttatg ctataatcaa gcgtgaaaat
    38161 gcagcgctca aaagttccgg aagacttcct gtctctcgcc ggtgcttgcg ggcaagaaag
    38221 gacttttccc atgcaaaagt tttatcgtct gtctgtaaat atcgtaaaga tcctgacact
    38281 gctgctggca gtttttctgt ttattggcag ctttctcact acctgttatg ccgaaaacat
    38341 ggagacccag caggtgcttc tgcgctttga caatcccttg tggaatctgc tggagcttgc
    38401 cggatacggg cttttatttg cctgttgtct gtctctgtcc ggcaaggccg gtgtgaaatt
    38461 ccgccgcggg ctgcttgttt ttgctctggg gctgatcctg cttctgggtg ggatgctcat
    38521 cgtcttcggc cgcacagttc ctgctgcgga cgccttatct gtatacaatg ctgccgctga
    38581 atggatcctt ggaaataaag atattatcca ccccaccttc tcttatctgt cctattatcc
    38641 acagcagatc ggcctgatgg cctttctgga gctgctgctc cgcctgtgga atctcaccgg
    38701 gctgagcgct cccgcctggc attttgtcaa gctggtctat gtctgcctgc tgtgcgtggc
    38761 cgttctgttc cagtaccgtt ccctgcgcta tctgtggccg gatgattggg aaccggtctc
    38821 ctgctgttat ctgattctgg tatgctgtaa cctgcctatg atcctgtaca gttccttcgt
    38881 ctatggcgag atcccttcct tcgcaatgct ttctgtggga ctatttcttc tgttaaagct
    38941 gttggcagac tgtattccgg cacattccgt ggagaccact tccccggacg acacccgcgt
    39001 cgtgggtact tcccacgcat tatccgcagt tactgtcttt accgcccttg gcagtatcct
    39061 gttccttact ttaagtgtga tgctgcgcaa gaactccctg atcctcatta ttgctgtgct
    39121 gctggtgctg ttcttcgaag cgctgcgtcc gggacgcagc ggcagggtgt gcattggcct
    39181 gatggctatg gcagtctgtc ttaccattac ctccgtgggc gtcctgccgc tggtgcagaa
    39241 gtgctacgag aaaaaagccg gcaatacctt aagttccggt gtcactgcca tgtcctattt
    39301 tgccatgggg atgcaggaat cttcccgggg atgcggctgg tacaacggct tcaatatcga
    39361 tacctatgat gctgccggga tgaattccga cgcagccaat gcaatcagcc gggcggcgat
    39421 caacgagcgt attgcctatt tccgggagca tcccggctat gccgtgaact tctatctcca
    39481 caagcatctg tctcaatggg cagacggtac ttatgccagc cggcaggcca ctctggccac
    39541 ctacggtggg cgaagcgact ttttgaagga agtctatgaa ggttctcttg cctctgctta
    39601 tatcgaatgg ggaaatgcct ggcagaatgt gttgtatctg ggcatgctga tgttctgtat
    39661 tgctacagtc agaaagcgcc gtcccggcaa tggctccgcg aatgccgcag ccaacgacga
    39721 tttccgtttc cgcaacctct gcctctacac ctatacggga ctgatcgccg tgctgggcgg
    39781 tttcctgttc cacacgatct gggaagccaa ttcccgctat attttcgtgt acagcctgct
    39841 gctgatgcct tactgtgccg ccgggatcca tgatggactg gtgcgtctgg cagcatggcg
    39901 cagcaacaga acgctgacac gtcactcaaa ttcataaaag caaaaaactc ccgtgaatcg
    39961 aataaaataa cattccattc ggttcacggg agttctcgtt tgtattttaa acctcaaaat
    40021 tcacaaataa aatacaaaaa tatgtttttt caagcctaaa aaaattatta ttttcgttaa
    40081 taaaatattt tgaacaaatt tcgtacaaaa aatcctatct taccatactt tttgtacgag
    40141 aatgcttata aacggcttat ttacgaaaat cctacggaaa cagtattttc taataataca
    40201 tggccccttg taaaaatccc cagaaataat acaatccaca catcagatgc cacagatagg
    40261 ccagccattg tatcgcaacc agtccctttt tcttcttcgt atccgccttt ccggtagccc
    40321 gcagcaatac caacagtgct cccacaaatg caaaaaagat accgtacata tatccccagg
    40381 aaaagttaaa atccatctcc cggaatccct tctcgtacag gcaaaacgcc atcagaaaac
    40441 tcataccgta aatctgccag gagaagcggt aaatgctgtc tctgcacaac tccttatagt
    40501 tcaacagaag caccaggatc gggaaaccga cagccagacc gatagccagc ggaagattac
    40561 tgcaatactg cagccacaca tgtcccagcg taaaaccgat gccgccttcc tgtccctcct
    40621 gcgggacgaa cacaccgcgg aactggtaca acagatccgc aaaagtagga aggaagcata
    40681 ctcccagcca aatggtgggc atgaagttcc ggaatttgct gtggaacatt ctccacagca
    40741 tcagaattcc cgcggccccc accagtacga tcgtaaagct cggcttcgcc atcgttgcca
    40801 gtaacagata aacagaaaag aggatataat ctcttccata ctccttgtgg gcattgtcct
    40861 gttcatacag aggcatcagc tctgcatact tgaaaaatgc taggattgca aagggtcgtg
    40921 ccgccatata agtagcgtta tggaaaggat ttgccgtaaa tactcccaga tacttgtact
    40981 tgatccccgg cagatagatt cccgttggcg gatataccat ggatacgaaa aacagggata
    41041 ccgctaccgt tcctgtgagt acgccgggaa gccaggcttt cccgggcatg gctttacgca
    41101 actccgcccc cacgtgcctg tccagcatta ttttcagggc gatcatcgct gcgctgttca
    41161 gcaacatcgc tgccagcgcc atggccagtt ccgttccggt gggcagactg cctgtgacca
    41221 gatggatcac tgccgccagc ttgaagagga tcggataggg aaagctgtaa ccgctgtcca
    41281 gtccctgcat ttccagaatg tatgccttca tatcggaatg atacagtccc gtccccaaca
    41341 tactttccac gcactgccgg taaaacagcc agagagtaac cgccgaagca atccccacca
    41401 gtacggcaaa cacaacgatc tctatgaaat tttcgacatt atttcttttt cccgtactgt
    41461 tttctgctgt tttctgtctc atgccgcctt ttcccgttat ccgtccaggt ttcacagtcg
    41521 gatcctattt cagcttcgtg taatatgcta ccactttttt caccgcatgg atcacatctt
    41581 ccacgtcttc gtcggtcaat ccgtagtaca gaggaatact catgacttcg ttgtagtatt
    41641 tctccgcatt cgggcacagc cctttttcat aacccagttt ttcataataa gagtgccagt
    41701 ataccggcag ataatgtacc tgtgggcagg tattctctgc atacagtgcg tcaaagaact
    41761 gccgtctgtc gcagttcaga ttctccagat tcagatgcag tacatacaga tgccaggtcg
    41821 tgtcagactc cgggatctcc ttctgcagga tcagttccgg catgtctgcg aaggcttcgt
    41881 tgtagcgttt gcggatttcc tttctgcgtt tctcaaaagc aggaagcttc tttaactggc
    41941 taagtaacaa tgctgcctga aaatccgtca tacgatagtt aaagcccagt tccacctgct
    42001 cgttgtacca cagagcatct gtaggatgta ccatttcctc cggatctctc gtaataccat
    42061 gagtcctcaa tctcatcaga tgacgatata gtttctcatc attcgttgta attgctccgc
    42121 cttctccacc ggttactgtc ttcaccggat ggaagctgaa gcaggtcata tctgccaggc
    42181 ttcccaccgg ctgtccctta taggttgtcc caatggcatg ggctgcatcc tcgatcagga
    42241 gcaggttatg ctctttgcag attttcctga tctcatccag ttccacagcc tgtccggtga
    42301 aatccactgc gacaattgcc ttggttctgg gggtgatcag cttccgaatg gatgccggat
    42361 caatattata agtctccggg ttaatatccg caaagaccgg ctttgcaccg gcatataata
    42421 cacagttgga agaagctgca aatgtaatgg agcttacgat aacctcatcc ccttctccaa
    42481 aacccgcggc cagagccgcc agatgcaggg ctgcagttcc gttgcatact actactgcat
    42541 atttagcatg tgtcaattca cacaaatgtt cttccagttc ctttacctta ggtccacagg
    42601 taatgtagtc gctggtcatt actgctgcca ctgcatctac atctgcctgg tcaatatatt
    42661 gccgtccgta gaaaatttta ttctctcgta caggttttcc tccaaaaata gctaattctt
    42721 ccataggggc ctcctttggt cactttttgc ctatttactg actcatctta tcatattgaa
    42781 gaaagaaaaa caacaatggt gttggcatcc accgctttgc tgcggtataa taaaaccgtt
    42841 ataaatccat acttactgtt cacagaaagg atctcttcgc cttatgagtt caaatacgaa
    42901 aaagagaata aaatcatgga aactcttttc cggtatcgtt attgtagttc tgctgcttgc
    42961 cgtaacagcc gccggaattc tctatgtgaa aatatatgaa cccggaaaac gttccgccga
    43021 tcttgggcgc ctgcagcagg aatcctatca gggggtcttc tgctccatgt atgcgccgga
    43081 agctttcccg gaagacattt actctaccta tatgggttac gatgctgtca gctgttccca
    43141 tcgcctgact tccttttccg atatctccga ttatctggaa actgcttttt cctccggaaa
    43201 tgaggtcact cacgtattgc tcattttgga ccccttactg ctgtggaaca gcaatttcca
    43261 taataacagt cgattttatg cctctttcga tgaggatctt ttggcatatg tcgattctta
    43321 ttccggtgtt gaatttacca tagtattctc ctgtccttct ctgaaatact ggcaggccca
    43381 cagcagtgac accgaaacct acgaaaatct ggtgcaggtt ctggcagctc ccctgtctgt
    43441 ccgggaaaat gtctctctgt tcttccccgg cggagaagaa tggctgatct ccaatcctga
    43501 tgcctatgac actcctctgg aattaaatgc tgtcgctgcc cgctccgtca tgctgctggt
    43561 attgagcggc accttgcaat accatggtat agatgccgat aactccagtc tgacacagtt
    43621 tgataccctg gtgcaggcag ccattaatgc ccctgcttcc tactccgatc tgtccgatta
    43681 taacatcgtc tttttcggtg acagcgtatt cggaaactat caggattttg cctccatccc
    43741 cggcgttatc ggtgccctga cgaaagcctc cgttcacaac cgtgccatcg gaggttcctc
    43801 cgccacacaa ttgacccccg atgacaaatc cttccccagt gccgtacagc gctttttatc
    43861 ggaagatact gccgaaatct ccggggagaa aaaactttgt ttcgtaatca attacggatt
    43921 gaatgactat tttacgggat atactgtgga gcaataccgt gacggtctgc aagcaggggt
    43981 gcaatctctg caggatgctt acccggatgc tgaaattctg atcgcttcct cgaatttcat
    44041 tacagcgttc gacaacggta ccgctttcaa caatgatctg gatgaaattc tgaacgatta
    44101 tgtgaccggt gccgaagaaa ccgcggcggc catgaacgtt ttctttctca ataccaacga
    44161 ggcactgcaa tggaatcccc aaaatgctgc ctgctatctg gtggatgccg ttcatcccaa
    44221 cgaagaggga cgtttcctgt ttggcacagc aattataaag gctttagagg aggatatttt
    44281 ttcttatcct gtacactgat atcttttccc agctgtactt cgatcacctg gagctccgtg
    44341 tctgccagaa gcatatgccg gcatcccgcc ggcatccgca gcacatcacc ggtctttact
    44401 ctgcgttcct caccatctat cactgcccgg ccttctccgt aaatcaccgt ccatacctca
    44461 tcccggaatt catggctgtg ataattcatc ttatgtccgg gattcaatgt cactttgatc
    44521 gtcagacttt ccttttccac atccatgatc cggaagctac cccaggattt ttctgcgaac
    44581 atgacctgct gggtaaaact gctcacatat ggtttgatat agcttgactg ctctttgtcc
    44641 gataccagaa tcccctcggg gctggccgcc acgacaatat cctgcaatcc catacacagc
    44701 accggcatat ccagctcatt caccacatgg acattccggc aggtctcatt gagcaccgcc
    44761 tccccgacgc atgcactgtc catggcttcc gtcagggtgt tccaggtacc cagatccttc
    44821 caggttccgg caaaacgcat gacctcgatc tcaggttcct tctctactac cgcatagtcg
    44881 aaactgatct tctgtagggt ttcatatttt gccagcaaat cttcataatc ggtaaactct
    44941 atgagttcat gagctttctg caacacgtaa ttcaggcgga aggcaaacac tccgccgttc
    45001 cacagcgctc cctgtctgat atattccgct gcctgctcct tgtccggttt ctccttaaac
    45061 atttccaccg ggctgatatt ttccgctgtt ttcggaatga tatagccata tttttcgctg
    45121 ggatacgtcg gttccatacc catcagcacc agatgggccg cactctgtgc cgcacgctcc
    45181 tccagccgcc gcaaagcttc gaaataatcc ttttccacgt acggatccac cggacatacg
    45241 atcaccggct cttctcccgt cactccctgc acatcctgca gatatgccac cgccagtgca
    45301 atcgccggga aggtgtctct tctgcagggc tccatgctga taccgacatt ttctcccagc
    45361 tggttgtgga tggaagagat ctgtgccttg gatgtggcaa tcgtgatctt tgcctccgga
    45421 tccgcctccc ggatctgccg gtacacccgc tgcaccatgg actccaggcc gccgtcttcc
    45481 gtatggaata ttttgataaa ctgcttggaa cggatctcgt tggacagggg ccaaagccgc
    45541 tgtccggaac cgcccgataa aagtatgata ttcataagcg tcctctctta attctgtacg
    45601 gtaagcagca gataatctcc taccgtatcc actacatcat aggaaaaatc aatgtattgc
    45661 agatctccct gatacaatac cagcaggccg tctcccacca tctacaccag ttctgctgcc
    45721 tgttgctctg gatagatgta gtgataccgg tccgcataag ccggaagctc tcttccgttg
    45781 gagacattac tcttcccctg ataatactgt gcgtctatct tacagacata ctgggtcagg
    45841 atctccgtgg catcccggtt gaactgccag cccagattgc cggtgatgta cagactttcg
    45901 tattcctcca gagaatccgc ttccgccacc gcctccatga aatctttgtt atagtactct
    45961 ctgctttctt ccgcataggt agtaaaataa gtcccgaaga acagaatact gctgattcca
    46021 taagcagctg cgaccaccgg ccacagcttt ttccacttcc gaaccgccag accgattccg
    46081 taggcacata acaggatgat cggatagaaa ataatattca cacggttaat gttaacttca
    46141 taagtgatca ggccgaccca gatcccggtg accaggaagc cccataatgc cagcatccgt
    46201 gtctgttttt caatgttttt ctcccggaag agctgtatgg taaatacaat gatccccaca
    46261 aagacaaacg ggatcgacac atgatacaac gggccaaaag ccggaatcga attgaagaaa
    46321 atatccggca gctgcaggaa tacatggctg aacagagccc aggcattttt ccccaactgt
    46381 gcaaaggaaa aattcaaaag cagaatatct gcgcttctca cactgtccgg aaaatacggc
    46441 atggtaaaaa gcggagtact tatggttctg ccaaagtgta ccgcgttgat tcccagaact
    46501 atgacttccg gcagggccac taccgtaaag atcagtgcgc acagcaatcc ttcccggaat
    46561 ttcagccggc gcttccacag gcaccatatg aagaatacaa aaagaaatgc agttacggaa
    46621 taggcagcga taccgtaaca gtaaaatgtc agtccgaaaa agcccatgga caagtataaa
    46681 tagcgtcgtt tttccaatcc aagaagcagt aaataaaatc ccagcaggaa aatatgcggg
    46741 aagagattgc aatccagcga ccagcggctc tgcataaact gccagggatt cacagcggtc
    46801 agcagcattg ccaccagggc gatccgttca gagaataatt tcctgcccac aagatatacc
    46861 aatgccaccg cgccggagga gatcagtacc atcggcagac gcaccgttac cgtgttgaat
    46921 ccgaacagtt tgataaaggg gaccatgcaa taggcaagga gaacgctcat ctgactgtat
    46981 ctccaggcag taaaatgaac cggcagaaaa gtaccatatc tgtcggttcc gtattttgat
    47041 aatgcccagg catccaccgc tcccatggcc tcgtcctggt tgataccgtc cggcactgtg
    47101 gcaaaacgga tgcaacgcag gatcacggca agcgcaagga tgctccagaa gaaaattttt
    47161 ctgtttttat tgttccaaag accggtggaa gaatcctgtg tcatagtatt cataatcgta
    47221 cgataccttt cgatgttctc tttcaggcct cctgcgtcat tttttccgcc gcatatggcc
    47281 tgtcactatc acatcatacc acagaatgtt gcatttgtcc ctagtcttcc gtataccggt
    47341 ccagcatttt gagatatttc aaaaccgctt tcttcttatc ctccggaaga ttacgatatg
    47401 tatgcaatat caattcttcc tcctgtgcat gtacttccac catttcccgc tcatcgaaga
    47461 tatctgtgat cgagattccc agctgattgc aaagaatcat catggtatcg attctgggaa
    47521 cagtcttgcc ggtcagaaaa cagcttaccg tagatgatga cagtcccgaa tgccttgcaa
    47581 tcttataagg tgtcgtgcct ctttcggcac agagctgttt gaatttttct gcaagttccc
    47641 tgcatttttc tttggtgtcg tacatattgt tttaccgcct ttgtcattta tttaggtctc
    47701 tataaagtac atgacaaaaa tcgacggatt cttacaaaat ttttttgtgt ggtgtacgct
    47761 gtatgcgttt cacaaaaagg aagcaaaaag acctgcctgt cggcagatct tttaagtatt
    47821 ataatatttt ctgttttaca tcatcagcag gctttcatga tttatttcat cataaataaa
    47881 ttccgcccat tcctcaacag tatcatggtc gacaaaatcc ttgtccattg ccagtgcctt
    47941 ggataaataa gaatcacgaa cggcacgagt tgcttccact tcattcaaat gatacttcct
    48001 gaccaaggta tcccgtacat aactcttaaa ttccctgatt tccgcatttg tcataccatt
    48061 caccttcttc cccttgcgtt caaatatttc ttcttgttat ttatcatatc acaagataat
    48121 acttttatgc aagatatcgc ttttaaagta tgaaaggaat actgttgcgg atatacctct
    48181 ccattatctt tcaaaaactc cttataatta atgtcttccg gtatgacttt gcctccgtta
    48241 accttatacg ctatattaat tatattgcca tccgcaattt ctccatagca aatatcgtat
    48301 tttctatcct gattgtgctc tagtatattg tacgcagaca gcatctgtgg tttcaatcga
    48361 ttattcagga caaattttcc ccatctttcc gaatgtcgtg gataggaacc tattactaaa
    48421 tccattgcac ttatcggttt ataatctact tctacaatgt atggttcctc gtttttatta
    48481 tttttcgcat tatatttctg agtagttctt attgctgcca gcgctgcatg atcatagctt
    48541 ggcgttgtat aaaaacccgg gccaaaatcc agatagggtt tactatattt cagatcgatt
    48601 ccatgattaa ctatatttat tgctgaacta taattggttc catgatataa tttcatctta
    48661 tgtttacttt ctttcacagt atttttactt caatatgttt ccctaatgca ctggcatatt
    48721 tttccaaaac caccaatgta ggaattcccg agccattctc cagtctggca atattagacg
    48781 ctttcatccc tgtacgatcc gcgatatcac tctgtgtcaa accctgtcga tgtctctctg
    48841 ccaccaattc cttgacaact tcatccgtat gactcgacac ttttttctgt attgcttcca
    48901 catatgcctg caatcttttt agctgatctt ttttccgctc ctcgtcatct cgcaaaattt
    48961 cagattttat gccattcata tcttctctta tatcgttttc tttcataatt catacccttc
    49021 ttctgtggtc ctgattactt tgttatttca tattatatca ttgatgatag tatctttcaa
    49081 gatatgtttc gaggaatatt tattcatata taaagtgccg gaattctgat tcgcagaaat
    49141 tacgttttcc atataatatg gtatgaattg caattctccg ctccacgatt cttaagttcc
    49201 ctcgatttat cgaaatccct ttagccgtca aaaactttac tccctcatta gataatgtta
    49261 tactttttat aaaacatccc gaaaggagcc tgatcttaaa tgcggaaatt tattatggat
    49321 aaatataatc tgattgcatt tgccatcgga ttgattccca ccattctact ttacctgttt
    49381 cctccggatg ccgctgtccc cttttgggca ttttctattg ttttattctt tctgttaatt
    49441 gccatctgga tgtgtgcaaa gctatggtta gaatccaaaa acgataaggc tgttcccacg
    49501 atatccatca tcgaatgcag tcacaatgtc tgtatttgta aaacacatga ttttatcaca
    49561 tacaactcga ttgttacatt ttataagaag gatgggaatt tcgaaaaatt aattggattt
    49621 ggattagtag aaactataaa cagcggtgga aaaaccgcac agattcggat cgttcccggt
    49681 gacatgcgga acaagaacca agtctccgct tcggaaattt ccgattttgt ctcttatatt
    49741 aatgacaata aaaataacat tattgtgcgc cctactataa caagcgatat cgtgccttat
    49801 atctataaaa tattaaatca ggaggtttcc aatgagtaaa cagattaaga tcgtacatgt
    49861 atccgatgat aattgtgatg tttctcttaa tgttggttct attaatgggg tgcaggaagg
    49921 ggacaaattt ctggtatatt ctctttccga ccacgagatc gtggatccgg ataccgggga
    49981 atccttagga tatctggaat ttgtgaaggg taccggggag gtcgtccatg tccaggataa
    50041 gatgtgcacc atcacatccg tcacatatcg gaaaccggta gccaaaaaga caatccgtaa
    50101 gacaacaccg tccatgacta tgtgggccgg cagtaccgtg gaagaaattg ttcaggatga
    50161 taaccgggag cagatacctt ttgacaatcc tcatgtggga gatcttgcaa aacctattta
    50221 aatactcttt tcaaaaac
//
LOCUS       DAWEJW010000006        45954 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_376_length_45954_cov_63.533606, whole genome shotgun sequence.
ACCESSION   DAWEJW010000006 DAWEJW010000000
VERSION     DAWEJW010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 45954)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 45954)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..45954
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_376_length_45954_cov_63.533606"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10388..10487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14634..14733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    26455..26554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    39146..39245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    42716..42815
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    44761..44860
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        10236 a        12593 c        10096 g        12429 t
ORIGIN      
        1 caccgatacg ctcgatatcg ttcaccacat ggaacagggc acccagactg ttcaaatcct
       61 cgatgggcag tgtggtctgg ttgatcttta ccagatagtc cgtgatggca tggttcagga
      121 aattgatatt tttctctacc tcgtagactt cctcgatgtc ctcttcatcc aacgtgatca
      181 gggcattcat ggcacggttt aagttctcct ctgccagaga agccatacgc tccagctcct
      241 tgataacttc taccactgca gtggcggggt tgaacaccac tttgtcgccg atgtatttta
      301 actgatagct ctctctgtaa ccaaccttct gatcctcgcc gggtacgatc aggtaagtca
      361 tcttgaccag ccatccggtg aacgggaaca gcataattac ctgtgcaatc ttgatcagcg
      421 tatgtgcatt tgctacgaat cgaccgttat ctgcggaaat ggacttgatc aattccacga
      481 tctggtctcc tgccacaaac agggcaatgt agataataac cgtaccgata atattgaaca
      541 gcagatgtat cagtgctgct ctctttgcgt cctttttacc ggtcatggaa gctaacattg
      601 cggtagcgca ggcaccgata ttacatccca ggataatgta cagcgtaata ggtaacggca
      661 gcagatcctg attagccagc aacaacacaa tgctgaccgt tacggacgaa ctctggataa
      721 tggctgtcag agcaaatccc atcaatgtgg ctaagaacgg gttcttcaga gacatcagca
      781 gattgaccac ctgtggctca ttcttcatgt tcgccatagc ctgggacatg gtgctcaggc
      841 ctacgaacag gataccgaaa ccaacgacca cttccgccac cttacggacc ttttccttct
      901 tcgtaaacat catcacgacc acacccacca gcaggatcag cggtgctatt ttggataaat
      961 taaaagatac cagctgtgaa gtaatggtag taccgatgtt tgcaccgagg ataacgcccg
     1021 ccgcctgata cagattcatc agaccagagt taacgaagct gacgaccata accgtacatg
     1081 ccgaagagga ctggatgata ccggtaaaaa taataccaac gatcataccc atgaaacggt
     1141 tcgtggtaaa gatttccaga atacgacgaa gtctggcacc tgctaccttc tcaatggaat
     1201 cactcatcaa ctccattccg aatagaaaca gtcccagacc tcctgccatt gtcaatattg
     1261 ttccaaagct catttttaat ccctcaatct ctcttgattc tcttccgaaa aaccaaaaca
     1321 ataattttca aatttattct tatataaaca atccaaaata tattttacga gatagaataa
     1381 aaacttgcaa tgtaaatttt acttttcttt tacttttctt tacaaaactt tacgcaattt
     1441 ctaccgtgtt tgcgtaaaac agctgttttt cctacgcatt tcgaccatcc gcaatatccc
     1501 ggtccagctg ctccttaagt tcttccagcg atccaaatct gcgttccggg cgccgaaatc
     1561 ttttcaaagc cacctcgatc tcctgcccgt agatgtcctg gtcaaaatca tacagataag
     1621 attccacccc catcactttt tcagaagaaa ccgtgggttt ggttcccaca ttgctgatgg
     1681 ccttatagac ctttccatta tggcgcaccg ccgcatagta aactccgttg ggcggcagaa
     1741 gtttgcctgc gggaggcagc agattcaccg ttggaaatcc cagggtatgt ccgatatgat
     1801 tgccatgctc cactgctccc accagcgtat aaggcattcc cagcatctct tcggcctctt
     1861 ccatccggcc ctcttccacc agcttacgga tgtaagtgga actgacttcg atgccgttca
     1921 cttcgatctt ctctatggtc tggacgcaaa agcccagatg cggcgccatt ctctccagaa
     1981 gttcggcatt tcctgcgccg ttcttgccaa aagacagatc ctcgcctgcc gccacaaacc
     2041 gggtattcat ctgtttggcc agaatctctg ttgcaaaacg ctctggctct gtggctgcag
     2101 tctccgtcgt aagaggaaac tcgatcagag tgtcgattcc cagacgttca aacaggcgtc
     2161 ttttttcctc tctggtggtc agttctttac cgtcagacag gccgaacagc actgccggtg
     2221 tagggtcaaa ggtgaacacg cacgccgcca gcccgtattt ttttcgacca agaatctctt
     2281 ccaaaagtct cctgtggccc agatggactc cgtcaaattt accgatggct actgctgtat
     2341 ctttttccag ataaaagtct gttgttcctg caatgatttc caagtctatt tcctgctttc
     2401 tgtattactt gggaagatct tcacgggctt gtaccattcc ttcgtttcat catacgcata
     2461 aatgcccacg aagctgtcat cctctctgta gatgcgcacc cactctcctg caggatgtgt
     2521 ggtctgttcc acagtctgtc ccggataaaa agcattcccg ttatcgatca gtttccgcca
     2581 cttttcctcc acatgcaaca tgggaaaatc cgaaaaaata cggtctgtgg gaagcagtgc
     2641 ttcctccagc ctctccgtgt cccgaagttc ctgcagctgt gcaagtgtca gggcatctgc
     2701 cagctggaat tccccgactc tggtacgaac gaggctctgc atggtgccgc cgcagcacag
     2761 tttttcccca atatcggcac agagactgcg gatataggtt cccttggaac attccacgcg
     2821 gaattttacc acgggaaact ttatttccag aatctcgatt gccggcagat ccacatgtct
     2881 tgcctgccgt tccacttctt tgcccgctct ggccagctca tagagctttt tcccattgac
     2941 cttcagtgca gaatacatgg gtgggatctg gtcatagccg ccaacaaaac cggcgatcac
     3001 ttcacgtacc tgtgcttcac tcaccgccac ttcccgctct tccaaaacgg ttcccgtgat
     3061 atcctgggta tctgtgatct gtcccagcag aagttctgcc acatattcct tggtcctgtc
     3121 cgtcagcatt tcacatgctc tggtagcatt ccccagacat acaggaagca caccggtagc
     3181 ctgtggatcc agtgtacctg tgtgccctat ctttttctgc cggcagatcc cgcgaagctt
     3241 ggctaccaca tcatgggagg taaagcccgc ttccttatat acattgatga tgccgtccat
     3301 taaccctgtt ctccccgcag ttgtttgtcg atgtgcagtg acaaattgtt gacacaatca
     3361 tggaaggttc ccttcatagt gcagcctgcg gcacgtacat gtccgccgcc tccgaagaaa
     3421 gatgccacct tcgccacatc tactttctca ttggaacgca tgctgacctt atactccagt
     3481 acattggtct catacatgaa aatcgcacag tccacgccct tgatattgcg caactggttc
     3541 acaatgccgt ccagatctcc cggtaccgca ttgtagaatt ccatggtctt gcggtccacc
     3601 atgctcacga tgcagcgtcc gtccatgaaa cgaatgctct ccatcagagc tctgcccatg
     3661 atctgggtct gcagataggt cttctggtaa aagctttcct cgatcagctt cgggaaattg
     3721 aatccgaagg agatcagaaa cgctgccgcc tgcagcgtct ccggtgtcgt attggaatac
     3781 tggaatacac cggtatcatg gatgatgccg gtatacagcg cttccgcaat atccttgtca
     3841 ataagttccg catccataat gtgatacagc acctcacagg cacttcccac ttccggacgg
     3901 accagattca catcaccgca accggaattg ctgatgtgat gatcaatgtt gatcttcttt
     3961 ttcgcattct ggaaatacgt ctccgcatct cccagacgat ccccactgca atccagcgca
     4021 aaatacacat caaaaggctc ctgcacggga cattcggtct gaatctcatc gtatcccttc
     4081 agataggaaa actcttcgga cggcttctgc agatatacgg taacttccgc ttcgggaagc
     4141 agtttacgca gatacatctg taaggaaaga catacaccca cacagtctcc atccggacga
     4201 atatgtccgc tgatgccgat ctttttcgca cccagacatt cttcacgcaa atccattact
     4261 cgtcaccttc ctctgtttct tccgtatctt ccaccacttc attttccggc ttctgtgcct
     4321 gttcatccgc tgcaatgacc tcatcgatct tgtgggacat attgacaccg tattcaatgg
     4381 actgatccat cacaaaagtg atatccggag tattacgcag attgatggta cgagccagtt
     4441 ctctgcggat aaagccttcg gcgctcttca gtccctgcag ggtagacttt ctgacttcct
     4501 cgtctcccag cacgctgatc catgccttac agctcttcag atccggtgcc acttccacag
     4561 cgaccacact ggtccaggga ctgatccggg ggtccttgag accgccccgg atgatctctg
     4621 ccagaacgcg ctgtacttcc ccgttgatac gggtattttt cacactattc tttctcattc
     4681 ttattccttc ttaattcttt cctgcgggta tcgtagacgt cttggcgagc atccgcgaac
     4741 agaatgcgtt tcgcgtaacc ccgttgaaaa acgtcggcac ttgaatcttg caagcaaagc
     4801 gcaggaagat tcttagtgtc cgctacgttt tttgcagagc gcgagcgtgg gtaagcggaa
     4861 tgcatctgcg aacggatgcg ttctagtaac ctaccgtggt acttccacca tgatatatgc
     4921 ttctacgata tccagctcct gcatcttgtc aaagccttcg aatacgagac cacactcata
     4981 tccggcctta acttccttca catcatcctt gaatctcttc aaggaagcca ggttgccttc
     5041 gaagatctgc tcgccctcac gggtgatacg aaccttacag cctctctgga acacaccgtc
     5101 cagaacatag gaacctgcga tgttaccgat agcggatgcc ttgaagatct gacgaacctc
     5161 tgcatgaccg ataaccttct cctcgtatac gggatccagc atacccttca tggcagcttc
     5221 cacatcctcg atagcctggt agatgacctt gtacagacgt acatccacgc cctctctctc
     5281 tgcggttgcc ttggcagtcg catcgggacg aacgttgaat ccgatgatga tcgcgttgga
     5341 cgcacttgcc aaagatacgt cggactcatt gatggcacct acgccgccgt ggatacattt
     5401 aactactact tcttcgttgg acagcttcat cagggactgt tttacagcct cgacactacc
     5461 ctgtacgtca gccttgacga tcaggttcag ttccttcaga ttaccctcct gaatctggct
     5521 gaacagatca tccagggaca ttctgccctt ggtctcttcc agcatctttt ccttgttctg
     5581 tgccagatag gtctccgcat aggattttgc tgtcttatca ttatcatgtg ccaggaatac
     5641 ttcacctgca ctgggaacat cggacaggcc caggatttct acaggggtag acggtcctgc
     5701 ttcttttact ctgcgtccct tatcatcgat catggctctt accttaccgt gtgccacgcc
     5761 tgcggaaatg aaatctccca catgcagggt acctttctgt accagtacgg tagccacggg
     5821 accgcgtccc ttatccagct ctgcctcgat gaccagacct cttgcttttc tcttggggtt
     5881 ggccttgagt tccatgatat ctgcggtcag caggatcatg tcaagcaact ggtcgatacc
     5941 ttctccgctc ttcgcagata ccggtgcaaa tacggtactt ccgccccaat cctcggggat
     6001 cagctcgtac tctgccagct cctgttttac acggtcaatg ttggctgagg gcttatcgat
     6061 cttgttcact gctacgatga tctccacacc tgctgccttt gcatggttga ttgcctctac
     6121 ggtctggggc attacgccgt cgtccgctgc tactaccagg atagcaatat cggtcgcatt
     6181 ggcaccacgc atacgcatgg cggtaaatgc ttcgtgtccg ggagtatcta agaaggtgat
     6241 cttacggtct cccacattta cagtataagc accaatgtgc tgggtgatac cacctgcctc
     6301 acgatcggtt acgttggtct tacggatagc gtctaacagg gaagtcttac catggtcaac
     6361 gtgacccata acacagatta cgggaggtct ctccaccaga tctgcctcgt cctcttcatc
     6421 ttccttcaac agttcttcga tgacatctac cttgacctct ttttcacaga gaatgtcata
     6481 ttccatggca atgttctctg catcttcata ggagatctcc tggttcacgg ttacaatctt
     6541 accttccagg aacagcttct tgatgatggc tgcgggctgt aacttcatct tctcagccag
     6601 ttctttgatg gtaatggatt ccggcagcac gatcaccttg atctgctctt cctgtacctc
     6661 ttccttcttc ttttccggct tgatgaaacg tccgggcttg ttcttcagcg cgtcctcgtc
     6721 ctcgtagata tgatccttct tggaacgctt gttgtccttc tcctggctga aacgtctctt
     6781 ttcatcttcg cgcttcttct caagatcctt gcctgcaggt tccggagcga agcccttatt
     6841 ctgtctgtta tctccgaatc gattacccga tccgccattg cctctttggt cgcggccgtt
     6901 attgccgtaa ctgcgctcac cattgccgtt accgcggcga tcaccctgac cgccttccat
     6961 acccggacga ccctgtctgt taaatccgcc tccctgaggt ctgccattgc ccatacctcc
     7021 tctgttacca ttctgtccgc cctgacggtt accctgtccg tagccaccct gacgattgtc
     7081 acgattgccg tctctgttgt cacggttgta accgccctgg ccacgctcgt tctgtccacg
     7141 gttgtcacga ttattctgac cgtaaccgcc ctgacggtta tcgcggttgt cacggttacc
     7201 gttctgaggt ctgtctgccg gtctggtctg tgccacattc tgtgcaggct gtgctgcctg
     7261 aggtgcctgt gcggctgcag tattctgtac tgcctgctcc tttaccggtg cctgtgtctg
     7321 aggctttttc gcaggctgct gtccacggtt gccgggtacc attgccacgc ttggagtggg
     7381 agacggaggg gtcagcggct tgatgggacg tacgggagcc tgtgcctgaa cacggttgcc
     7441 gtaggaatta ttctggtaac cattgttctg gtatctgccg ccctgattcg gacgctgtcc
     7501 gcctgcattt ctctgtccct gtccactctg tccactctga ccaccctgac gctgtccggg
     7561 catcttggaa ttctgagggt tgctaacgaa aataatcttt ttcttctttt tcgtctcctc
     7621 agtagatttg gtctcctcgg aaactttcgt ttcctgagcc tttgcttccc ttgcgggtgc
     7681 ctgagccttt tcctcagcag gctttgcgtt ttcggtattt accggagctt tctgtgctgc
     7741 gggtgcagct gcttttttct ttccgaggct gttgcgtacc agtgctgccg catcctcttc
     7801 cagcgagctc tgtgctgcct ttgcctcaat tcctttttcc tgtaaaaaac ttaatatatc
     7861 cttactctgt agttccaatt cttttgcgag ttcatgtact ttgattttcg ccatgctttc
     7921 ctccattctg cctaaccggg caaattcgct tccaattgct tgatgagtgc atctgccagt
     7981 cccgcatcac acacacctac cgaagatctc agatcccggc cgatggccca gccgagttct
     8041 tccttcgtgc catattcata taccgggact tcataatagg aacatttgtc gtgaaacaac
     8101 tttttcgtat tggccgatgc atcctctgca acgatcacca acatggcaga accttttttc
     8161 acagcgtcct ccgtctggaa ttcaccgctt accaggttac gccccctcat cgcaagtccc
     8221 aaaagggaat aaatcttatt ctgtttcaag ctgatcaaac tccttttcca gtgtatcata
     8281 tacctcagga gaaatactca tcttgaaaga acgttctaat cctttattct tccgggcttt
     8341 ctgcagacat tccttctgtc tgcatatata tgcaccacgt ccgttttttt tgccggttgc
     8401 atccagacat atctcatctt ccggtgtttt caggacacgc agcatttctt ttttgccttt
     8461 catttctccg cagcctacgc actgcctcag tggaatcttt tttgccattg attattcctg
     8521 ctctccatca aaatcgtcta cggtatcatc ttcgtaatca tccacatagt cctcataacg
     8581 gaagccggga gcatccttgg cctgagtctc ggacttgatg tcgatcttgt agccggtcag
     8641 tcttgctgcc agtcttgcgt tctgaccttc cttaccgata gccagagaca actgataatc
     8701 gggtacgact acctttgctg tcttctcatc gggatctgca aatactgcta cgatcttggc
     8761 aggagaaagt gcattctgaa tcaggttacc ggggttctca tcccagttca cgatatcgat
     8821 cttctcaccg cgcagctctt ccacgatagc attgacgcgg gcaccattca tacctacaca
     8881 ggcgccgaca gcatctacat tggggttatt gctccataca gcgatcttgg tacggcttcc
     8941 tgcctctctg gcaatgctct tgatctctac ggtaccgtcc ttgatctcgg taacttcgga
     9001 ctcgaacagt ctctttacca gttccggatg ggtacggctg acattgatgc ggggtccctt
     9061 cggggtgttc tttacttcca caatgtaaac cttgatacgc tcggtgggct tgaatacttc
     9121 gccctttacc tgctcgttct cggtcagcat ggcatctgcc ttacccagat tgatgctgat
     9181 gttcttgcct acataacgct gtactacgcc ggtcacaaca tccttttcct tctcgtagta
     9241 ctggttgtag atcacgcttc tctcttcctc acggatcttc tgtaagatca cgttcttggc
     9301 attctgggta gcgatacggc cgaactcctt ggacttgatc tccacatgga tcatgtcgcc
     9361 cacctgtgct ctcttggaga tctcctgtgc atcctccaga gcaatctgca ggcagtcatc
     9421 ctccacatca tccttcgtgg gaacgacttc cttctcagca tataccagaa aatcgcaggt
     9481 ctctctatta atctccacat ggacattgtc agcctttcca aaatggttct tgcaggctgt
     9541 catcagggag ttctcgatgg cttcaaacaa ggtctccttg ctgatctcct tctccttctc
     9601 cagaacgtcc agtgcctcca ttaattcctt attcatcatt tcctccattc cggctttcgc
     9661 cttttcttag tgtatggttt cttcttattc tacaagctat cactattcct tcgccctttg
     9721 gctctgaata gtatcaaaaa tctaatgcaa gacggatcag ggcaatgtct gctctggcaa
     9781 aagttctcgg gctgccgtct tccgtgatcg taatgttatc tgcattgaag ctctccagaa
     9841 tgccagaaaa ctctttgcat ttgtcaatgg gcttgaacag cttgatctct acttcttcac
     9901 cgatgctctt ctgcaaatgc ttatcctttt tcagcgttct acccagtccc gggctgctga
     9961 cttccagaat ataagcatcc ggaatgaaat cagccttgtc cagagcttcg gataatgctc
    10021 tgctgacatt ctcgcaatcc agaatgttca caccttccgg cttgtcgatg taagctctca
    10081 gatagtagtc actgccttct tttacatact ccacatcgta gatctccacc tgacttactt
    10141 ctgcgatggg cgtcagcagt tcttcggttc tgttttcgta gtcctctcgt ttggacatgt
    10201 aggccctcct ttactgatat gagctgtcgt tacaacaaca agaaagagtg gcgcgcgtcc
    10261 actcttactc tagtaatcat ctttaaacta tacacagtat agaccctgtg tgaaaaaatg
    10321 tcaatagggt cttgcaaaca ttcggattcc gctgatttgc ttcgcaaatc gcttcttcct
    10381 catgtttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gcaggcgcat
    10501 gcaagcatgc ccatacattg taatacccac gaattgctcc gctgccgtgc agcttccgca
    10561 attctgcaaa cattcggatt ccgctgattt gcttcgcaaa tcgcttcttc ctcatgtttg
    10621 ggcggttccc tgattcagcc gcaggcgcat gcaagcatgc cctgcggctg aatcggtcac
    10681 ctggtattac aagtagttat gtgtttcaaa gtacagttcg ttcacgtgtt cggaatgcaa
    10741 aagcttatgg gcggtgcggc tgaggggctt gcccaggaac tcttcgtagg ccttctgtac
    10801 ctcggggttc tcatgggaga agcgaagctt tctctcactg tccagacggt acagggtgcc
    10861 tccgcgccgc tccgcgaatt cgcatccgtc gtggatgggc tgtccgccgc ctccgacaca
    10921 accaccgggg catgccatga cttccacgaa atcataggat acccttcctg ccttaatgtc
    10981 ctccatcagc ttctccgcat tggccagtcc gctgacagta caggtatgta agatctgatc
    11041 tcccagtgtg aagtctgctt ctcttctgcc gtctcttccc cggactgccc ggaaagcatc
    11101 tgcttccgga ttcttgcctt ccactactgc atatgcggta cgaagtgctg cctccatgac
    11161 accgccggtg acaccgaaga tcactccggc accggtgctt tctcccagag gagaatcaaa
    11221 agcctgctcc tttaagaacc gggtattgat atgctcggca cggatcatcc gcacgaattc
    11281 tcttgtggtc agtacaatat ccacatcctg tccggctccg gcgctcttca tataagaaag
    11341 agaagcctct ctcttcttgg atacgcaggg catgatggag atacagcaga tattattggg
    11401 atcaatccct ttcttctgtg caaaataagt cttcgccatg gcaccgaaca tctgctgggg
    11461 ggattttgcc gtggacaaat tccgcagata ctccggatac ttcttggaca cgaagcttac
    11521 ccagccggga cagcaggagg taaacatggg ccatgcgtat ttccgggaat tgcccagccg
    11581 ctccagcagt tcgtttccct cttccataat cgtcagatcc gctgcgaagt tggtatcaaa
    11641 tacatagtca aaaccaatgc gcttcagcgc tgccaccatt ttgccctcgg aagcttcctc
    11701 cgcattcagt cccagttcct cgccccatgc ggttcttacc gccggtgcca cctggactac
    11761 tgtgaccttc tcgggatccg ccagtgcctc gaaagccttg tatgtatcgt ctcgttcccg
    11821 caatgctccg gtaggacaat gggtaataca ctgtccacac agtgtacagt cggagcaatc
    11881 gatggtctta ttatccgcca catctactgt ggtacgggat ccggtgttct gtacatccca
    11941 gacatgcatg gcctggactt tatcgcagat ctgtacacag cgcatacatt taatacattt
    12001 tctggaatcc cggatcagag ggaaagaatg atcccagggt gtctcgggaa cttcctcggt
    12061 aaaaggtact tccagaattc ccagatcgtt ggaaatctgc tgtaaattac agttacggct
    12121 tctgacacag gtggcacagt gacaatcatg ctgggacagg atcagttcca catttgtctt
    12181 gcgcacacgg cgtaccttcg gagaattggt atacactacc attccctctt ccaccgggct
    12241 gttgcaggct gtgatcagct tgcttttttc ctgcacttcc accacgcagg ccttgcaggc
    12301 actgatctca ttgatgtctt ttaaaaagca cagatgcggt acttcgattc ccacgcctgc
    12361 tgccgcgctc atgacgctgg ttccctccgg gacctgcagc tgcacgccgt ctattgtaag
    12421 gtttatcatt ttctcgccca ttgtttgctc ctctttttac cagatggcag ctcggccgcc
    12481 ctttaagctt ccgtagccgt atttatcgca gcgtaagcat ctgcccgctt cctggcaggc
    12541 ttcctgcttc gtcatgccgc attcaaatgc atcgaaattc gtcttgcgct ctcctgcttc
    12601 cttttcactc agctggatcc gtccgcaagg ttccttgtcc tcgtaggata ccggcgggat
    12661 ctccacatca cagggtacta catggtggta acccaaatat tcatcgatat tggctgctgc
    12721 tacttttccg gcggcaatgg cacggatgac ggtagccgga ccggtcacac agtcaccacc
    12781 ggcaaataca ccgtggacat ccttgacttc actgtcactc atggcagtaa tggtgcctcg
    12841 ttttacggag atacccgcct cctcgaaatg gcgggtctcg ataccctgac cgatggccac
    12901 cacgacgata tcacagggga ttcgaaggag aggcacatcc gcctggatcg gacgggctcg
    12961 tccccaggca tcgatggggc cgatgatctg cggttctacc cacagagccg tcacattgcc
    13021 gttctcgtcg gcttcgatct tgtgaggtgc tttcaggctg taaagctctg cgccctctgc
    13081 gatggcgcct tccacttctt caggaagcgc cgtcatatcg gtctgtcttc tgcggtaggc
    13141 aattcccacc tttttcgcac ccagacgaat ggcggaacgg gtacagtcca tggctacgtt
    13201 accgccgccg actacgacta cggtcttgtc cttgaagtcc ggcatgtcat cgtcaccgat
    13261 gcggcgcagc atttccactg cagacatgac accgttggca tcctctccct cgataccgat
    13321 ctttttatcc gtatgggcac cgatggcaat atagactgca tcgtattctt cgtgcagctt
    13381 attataagaa atctcacctt cgccgttgcc gactctggtg ttcaatttca cttccactcc
    13441 ggtggacaga atagtgtcaa tgtcctcctg taagcgttct ctcgggaagc gatagttggg
    13501 aattccgtag cgcagcatac cgcccagctt gctcttttcc tcaaatacca cggcatggtg
    13561 tcccattagc tccagataat atgccgcaga cagtccgccg gggcctccgc cgatgattgc
    13621 tactttttta ccggtgctct ccgctttctc aggcacggga accgtgttgg ctctggcatt
    13681 gtccactgcc atacgcttca ggccgcggat attgatggca ctgtcgatca tgttgcggcg
    13741 gcatcttgcc tcacagggat gctcacagat cagggcacag gcggtgggga aaggattgtc
    13801 cttgcggatc agcttcacgg catccgcata acgttcttcc ttcaccagtg ctatgtaacc
    13861 ggggatatcc acacctgcgg ggcacagagc cacacaggga accggctggg taatatggca
    13921 ggaacatttt ccgccatgtt caatgtggta gacataatcc tcacggaagc cttccagtcc
    13981 ggccagtacc atatgggccg cctcgtaacc gatggcacag tcagcggagt ccacaatgtc
    14041 ggaagcggta tcccggatca gatccagtgt cttcagggtg gctttgcctg ccaatacatc
    14101 ctccatcagg ttctgcaact gccccagtcc cacacggcag ggcacacatt taccacaggt
    14161 ctgggcatga cacattttca gaaaagaaag ctgcaggtcc accggacata atccgggcgg
    14221 gctggcgata attcttctct ccagatcttt gtacaggcgt tccaccgtaa gctgtgcttt
    14281 attcggggta gctatcttca atcggctcat aatcttctcc attctgcggg ctgatgcctg
    14341 cgttactttt atgccctgcg ccacgggcgc cgccggccac ggctatggcg tgctgctctc
    14401 cgtctgcggg tgcggtgttt tactgacacg tactatggca ttggccacct gagctggctg
    14461 cctgttgcgc cgtgtaagtg cttacatttt attttttaaa aaatagccgt ttcttgtcgg
    14521 ctactgattt ttgtatatgt gcaattgtat cctaaataat aaaaactatc aatagttttt
    14581 aggaaagtta attttttatc tatctttttg tggaatatgc atgaggatgt ttgnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagcccag gcggaacagg ctgtcagaaa
    14761 caaaaaatga caggctaatc cgccggagct atattgccta tgatattaat ctttttacac
    14821 cgtcacgatc acgccgccat cgttggcgta catgctgctg acaccgcggg tgtccatgat
    14881 caggcatcct ttgaactctg ccttttccat aagaagcttc ttcatggtct cggcgcggtc
    14941 aggagcattg cagtgggtga tcatcaggaa acgttccttc gtatcccctg cctcttccag
    15001 aacgatctcc gccattcttc ggagtgcttt cttcatgccg acgctctggc ctctctgcac
    15061 gatactgccc ttgtctccgg ccatgatcgg cttgatgttc agcgtggatg ccaccagtgc
    15121 tttcacaccg gtcagtctgc cgttcttgcg cagcgtctcc agattatcca gtacaaaata
    15181 ggtatgcaca ttgctgcgga actcttcgat ctgcttcacg atttcttcga aggaaagacc
    15241 ctgctcttcc agttctacca gtttcttcac gatctgggtc tcaccacagc tggcggactc
    15301 ggaatcgatg acgtggatct gcttcttccc gtatttttcc cgatacagat tcataccaag
    15361 caccgcactg ttgtagcttc cgctgaggtg ggaggataat gtgatcacat acacatgctc
    15421 cgcttctgtg tggtaggctt ccctgaaacg ctctggggaa gggcatgccg acttgggaca
    15481 tttcggacac tctgccacct ttttcaggaa ttccgcctga tcaaaattct catcatcctg
    15541 aatttggtaa tctcccactt ccagtcccag gggcacgatc tcgaaccggg gatcctgcaa
    15601 atattcttcc ggcaattcac aacagctgtc tactacaatt ttataactca tattatctat
    15661 ctccatatga ttgattgcag ctattcaaaa ttatgtctcc gaaagagtca ttctgcgaat
    15721 gcggcactga atagttacaa ttattttcat tttttaaaac catactatgt agtttttaat
    15781 actacttata ataacacgaa acattccgtt cgtaaagcct ttatacaaaa tattcatatt
    15841 cccttttcgg cggacttcgt atatactatt tctatatagc cagcatatta tatgatgcca
    15901 gggtcaaaag gtctaagccc atatgagctt gctcataagt gggtgaaaga ccttaggacc
    15961 cgccccgata tgagcataaa agtgggatga taatcccacg atatgcgaat agagggtagg
    16021 attgttccgt ttcgaccgaa gctcctcaca gagttcttct cacaaacata taaggagtcc
    16081 ctatgatcgc attacagatt acatccatga aaaattttat gaatcacctg ctggtagcgg
    16141 atactttcga tcctttcctg ctggaagaag ccaccgtcag tacggctctc accgttacca
    16201 ttgacgggca tatcaacaag gacttctatc ccgcagagga acggagtgca gactgtatcc
    16261 cctgggagct gcagtcctgg agcaaaataa aaggactgtg ctttgacctc atcaagggaa
    16321 aacacactcc tttgtatttt aaattcgtgc ttcatatgca gccggataaa gccgccacca
    16381 tgcttacaaa agcccaggtg gaccctgacg tcatccgagc actggtgctg aatatccgct
    16441 acgacgggga aaagaccgtt ctcaccaccg gatgtgccta ccaggtcttc accatggata
    16501 aaagtgccga caccgcatgg gacaaggcat taaaaaaata tctggatctg aagggtatct
    16561 cctacgaaga actctgatcc cgctctccgg ggtatcccca gttcttcgaa tcattcatag
    16621 gagcggttcc tgacggtcac tccttcatct tcggtttgct gagcaggctc gccagataac
    16681 cgaggatcag cgctgcggag gttcccacgg ctccagcctt gaatcctccg gcaaaaagtc
    16741 ctaaaaatcc ctgttcctgc accgcttctt tcacgccctt cattaataca tttccaaagc
    16801 ccaataacgg tacactggct cctgctcccg cataatcctg aaacggttca taccagccaa
    16861 gtgcactgat caccgcaccg gtgaccacca gaagcaccat gatgcgtccc ggcatcatct
    16921 tcgttttttc cattaatatc tgcaccaaag cacagatcag gccgcccacc cagaaagcat
    16981 tgatataatc cattctctgc atttccccct tactatctgt ttaaggacag tctctgcact
    17041 ttttcttaaa attatgcatt ctttattgat gcattctttt tgccaaaacg aaaccattcc
    17101 ggcggaagct gtaccaggat caccgccgca aatacgataa cgcagcctgc gatctgcctg
    17161 gctgtcagtg tctgatcagt agtcagaaat ccgatcttat aggcgatcca tccggagatc
    17221 gtggcaatga cggattccag gctcatgatc agtgccgcca ccgtaggatt cagtcctttc
    17281 tggcccacga tctgtaaggt ataacccaca ccacagctta aaacaccggc atataacagg
    17341 gctcccaggc cttcctgcag ctgaccgaag gtgggctgtc cccagatgag tgctccgatg
    17401 cagcagacca ctccacagac cgtgaactgg atacaggata ccacagctcc gtcaattctt
    17461 gctgcataat ggtcaacggt catgatctgt atggcaaatg caatggcaca taaaaatacc
    17521 atgatatctc cggctccgag acgcagggac tcggtcatgc agagcagata catgcccacg
    17581 gctgccatga ctgctgccgc ccagaccacc ggcgccactt ttttcttcag aaagataccg
    17641 aacatgggga caaaaatgat atatagcgtt gtgataaatc ccgccttacc gacactggtg
    17701 tatttaatac cgaactgctg caaggtactg gcgatcgtta ggaaaatgcc acacaaaagt
    17761 cctgctttca cattcagttt ccagtcaagg ggcgcagctt tgtcccctgc aatctttctg
    17821 ctgatcagaa tatacaccag aatgctggcg gcaccgatca gggaacggac accgttaaag
    17881 gtgaacggat gcatatagtc catgcccttg ctctgggaga cgaacgccat gccccagatg
    17941 gcagccgtta acagcaggat caggctgttt ctcgtagttt ttgttctcgt attttccata
    18001 gttgtatttt ccattcctta tatttttcgt tccttgctgc tttacggtta tgtgatcttg
    18061 tcagggctgt ttcttatatc tctcagcaat ctccgccact acatcccgca gcatttttct
    18121 gctacagtct atgatcagat ccgcatattt ttcatacaaa ggggttctct cctcgtacag
    18181 gtccctcaat gtctgaccgg gctgcagggt cacacctctg gcattcagat ctcccagccg
    18241 ctgttccact tcctcataag gaagcttcaa atataccacg gtgctgatat cccgcagatg
    18301 ctgcatgccc tccggctcgt agcaggcact tccgccggtg gcaatgatgc tgttttccag
    18361 attcagagag gcatttacgt tgctctcgat ctgccagaag ccctccacgc cgtgttccgt
    18421 gatgagttca tgcaataatt taccgtgtct ttcctggatc accagatccg aatccacaaa
    18481 acgtctgccc aggcgctttg ccagaaccac gcctaccgta ctttttcccg aacccggcat
    18541 gccgatcaga gtaatattgt ttttcataaa tttctgtcca ccttacctat acacaagaaa
    18601 atcgccgcct gtaagcgacg atcttccgtc ctgttcttct ttataacctt cgtagcgccc
    18661 catttgcaga actgcctctg tggggatttt gaccccgaca tcatgctatt tgctcatggt
    18721 ggttacttac actttatact tccgcaatga cttccacttc acataatacg ttcttgggaa
    18781 gggtcttgac tgcaacgcag cttctggcag gattctcagt aaagtacttt gcatagacct
    18841 gattgaaagc tgcgaaatcg cccatatctg ccagaaaaca ggaagtcttg gccactctgg
    18901 tgtaatcggt gcctgctgcc tttaacagct cgccgatatt cttcattacc tgctctgcct
    18961 gttcctcgat ggtagttccg tgaatctctc cggtagcggg atcaataggg atctgtccgg
    19021 atgcgaaaaa gatattgccc agtacaatgc cctgagagta aggtccgatt gctgcgggtg
    19081 ctttgtctgt tgaaatcttc tgtaacataa tattctcctc ttcttcctat aattacgccc
    19141 tgaggatgcc tccggatccc ggcacatcca aaatccgatg cagcatgcac atagcgttta
    19201 cttcgtcata tctacctccg gctcatcaaa ttccgccgtc acgtagaatc ctctggaatt
    19261 ctccacgata tccgccacaa ctccttcgcg attcttcaac cgataacgaa aaggaatatt
    19321 gggggacatg gccagagacg ggtctatcgt ataataatag ttactgggca gaagtccctc
    19381 ggtcactgtg atcttctgtc cgacctccaa cagtcccggc cgctccatct taaattctat
    19441 cctgcgcatt ttgactcacc agccattccc ggcataatgt gttttcgccg tttgaggttt
    19501 attttaccag taacttacag cgcttgcaag agtgtgtttc atttctgacc tgctgctttt
    19561 ccctatttca ccgccggact ctccaatact accgcatgtg cgatccccgg cacgctttta
    19621 ccttcgttga aactggtctt actgagcagg gcgccagtgg gtagaaagag gacttttttc
    19681 cactcgccgg attccagctt cggcaggacg tatgccgcca aggtcacagc gctgcatcca
    19741 cagccgctgc ctccggcgtg ggtgtcctgg gcttctgcgt caaaaatctc gataccacag
    19801 tccatgtgcc ggtcggcgat gtcaattccc ttttcccgca aaagatcgat caataccgtc
    19861 tgtcccacgc ttcccagatc cccggtgatg atccggtcat attcttccgg ggtcacatga
    19921 aaatcttcca tgtgacgggc aacggtgtct gcagcggccg gggccatgca tgctcccatg
    19981 ttcatggagt ctttcaggcc gtaatccacg atccttccgg gagtcatgcc ggtgatgagg
    20041 gctctgcact tctgatttag tcccagggca aaggctccgc ttcccgtcac cgtccagctg
    20101 gcagacagag gtctctggct cccataatct aacggaaaac ggaactcctt ctccgcactg
    20161 gcaaaatgac tggaggtcac acagaccaca tgctccgcat agcctcccgc tgccatcaca
    20221 gctcccagta tcagtgcttc cccgcaggta gagcaggcgc cgtagactcc aaacagagga
    20281 atattgtaag cggagatacc gaaggaagtg gcaatgcact gccccaacag atcaccggcg
    20341 aagagatacg acacatcctc cgctttccat ccggcctttt ccatgaccat gtagactgcg
    20401 tccttctgga gattactttc cgcctcttcc caggtcttac agccgaagag atcatcctct
    20461 cccaccatgt cgaacagggt gcctaacggt ccttcgcctt ctttttttcc cacgatactg
    20521 gcaccggcga tcacacacac cggctgtgcc agacggatac tgcttcttcc cactctctga
    20581 ttcataattt ctccttccat gtgataacac tgcattcttt tttgtcagtg ttcccgcatt
    20641 agggaagaat tatgaattac aaaaaattgt aactattcag aaccccattc gcaaaaccgc
    20701 tacttcgtag ctttcctttt gctcatgtgg ttacttcgcc atataaaact gcttatctgc
    20761 ctgaatgcaa gcattcgcag aacgcagttt ttcatggctc tgaatagtta caaaatatct
    20821 cctccagcgc cccgatcacc ttctgtaata tctcatccgc catatccgaa tagtgaatga
    20881 ccagaatccc ccgatatttc tgcgcatccg caaagcaata ttccgacaga cagttcacga
    20941 gaattccccg ctgtctggcc gcccacttga tctccacgtc actaagttcc gtccgcacat
    21001 gcagcagaaa atgagtcccg gcatcatcct gatagatctg gctgatctga cttaaaggtg
    21061 actcccgaaa catccggcag atccgctccc gctgccctct gtaatcgttg agcagtctcc
    21121 ggatatggcg ttcaaaatac cctttttcca gaaacgctgc catggcatac tgctcaaaac
    21181 cggataccgt gcaggaataa aaattcatag tggaaatata gcgttccatc aatttctccg
    21241 gcagcaccat atagccgata cgcagggatg gcaccatggt cttggaaaaa gtgttgatgt
    21301 aaataactct gtggttgcgg tccatggact gcatggaggg aaccggcttt cccaccatgc
    21361 ggaattcaca atcgaaatcg tcctccacga tatagcgctc cggttcctcc gccgcccagc
    21421 gaagcagttc ctgccgtctt cccacaggca tgaccagtcc gatgggaaaa tgatgttccg
    21481 gcgatacatg cgccacggta ataccctttt gatccagctg ttccactttc attccctgac
    21541 cgtcaatgtc cacataatcc caggtcacac cgcccacatc atacagtttc gtaatcttgc
    21601 gatacccagg attttccaca ccgaattttg catttttgcc caataactgt agcagacgac
    21661 tgtagagata ttctgttccc gctcccacaa taatatgatc cggtgacaca tgcatgccac
    21721 ggtaccgata caaatgctct gcaatggcca cccttagttt ttccactccg ttaaaaggaa
    21781 ccgtcttaag cagagacgta tcataatccg tcagggtctg ccgcatgatc ttcgcccagg
    21841 tggcaaaggg gaacttatca tattgaatat tgttggccgt cagatccgcc agatattctt
    21901 cctcccggaa acgggtcaca aaggatgcat aggctggggc ggagacgttc ttcacttcca
    21961 gacggttgac aaaataccct ctcttttcct ccgaccggat atagccctcc agcagcagct
    22021 gttcataggt attctccacg gtcttgacac tgacctgcaa gtgctcggca aacgcccgct
    22081 tcgaaggaag cttctcaccg gcagtcagcc ggccgctcaa gatgtcagct ttcatcgctt
    22141 catataagta ttcatacatg gtttttctgc cacgggcagt cagatcatag gtcagcatct
    22201 ggtatcctca ttttttcttg ttttggttat ttaaaagata tcagatttta ttataatgaa
    22261 ctcataaagt aaaagcaagc aggaaggaca gattctcccc ccgccaaggg ggtgactcct
    22321 caagagggta gctcctcaag aagagcagga ttttccttcg gaaaacaagg gaggatttaa
    22381 catgggcgta tatgcaatca ccggcgcatc cagcggaatc ggcgccaaga caaaagaact
    22441 gttacttcaa aaaggacacg aagtcatcaa tatcgatctg aaggacggcg atatctgcgt
    22501 gaacctggca accccggaag gaagacaggc cgctgtagac aagctgcatg agctgcatcc
    22561 cgaaggtctg gacggcatga tctgcaatgc cggtgtctcc ggtgcctgcg gcaatctgga
    22621 actgatcatt tccctgaact atttcggaac cgtagccatt gcaaagggcg tctatgacct
    22681 ccttaagaaa aaacacggca gctgcgtagt caccgtatcc aacaccattt cccagggtgc
    22741 gggacgtaaa gatatcgtag atctgctgaa caatatcggt gatgaaaaga gagtattaag
    22801 cctggtatcc tccatggatt ccaccaacct ctccgtgggc aacagtctct atgtatccac
    22861 caaatatgct ctggccagat gggtccgcag agtctccgcc acctgggctg ccaacggtgt
    22921 ccgcatcaat gcggtagctc ccggcaacgt acatactgcc atgaccgcta ccatgagcac
    22981 caccgcgaaa atggcactga atgctctccc cattcccacc aagtacggtc aggaatgtct
    23041 gatggctccc gaagagatcg ctgaggtgat ggtattcctg gcatccgatt ccgccagagg
    23101 tgtcaacggc aacatcatgt tcgtagacgg cggtactgac gcactgttaa attctgaaaa
    23161 ggtatattaa ggaggaacac gctatgagac ccattgaaaa atatgtagaa gctatggaaa
    23221 ataaagattt tgccggactt gcagagttgt tcacccctga tggcattctg tgtgactatt
    23281 gtaccacctc cgcctcccag caggaatatc atatctacgg aaaagaggcc atcaatatgt
    23341 tcttccgtaa caaattcggc ttccgccgct actccattct ggatgctgag gtcgtaaatg
    23401 atgagcaggc cgagttcatt gccaacttcg gaggctacaa catcatggcc atcgccacgg
    23461 ttcgtaagac cgatgaaaac ggtctgatcc agagacttac cgtaagaccg aaataatttt
    23521 tgccatcccc ttatactttt ctctatatag aatcttaatg taaaagaagc tgcaccctac
    23581 gatacagctt cttttacatt ctatcttccg aatacgtcgg tcattccgga gcactgccag
    23641 agatcacata gctggtcacg gcatacagga tctcaccgtt gtactccact ctggaccagc
    23701 cagatccttc actgtagccg gtacgatgca gattggttcc gttccgcacc tgggtgcgta
    23761 cagtgctctc tccctcgctg gtgcttggtt ccgtccgcag attgatcaga tctttggggg
    23821 taatataatc atcacaatcc ataaagacga tcactctgcc gtcctgggtg ctgacgcggt
    23881 tgggatatgt cggcttctcc ggggtcttat aggtaagatc cgtggtcaga tatgctgata
    23941 ctgcataaac cgtctggccg ttgtattcca gtctggacca tccggtggaa tcgttccttc
    24001 cggtgcgtac cagagtctct ccgttcttca gctgccctac cacattgccc tcatctccgg
    24061 tatagggtgt actgcgaaga ttactcacat ccttggcagt gacagtctcg ttcacatcac
    24121 cgaacgtcat ggaatttccc tccgcaagag catctcccgt attattggta gtggggactt
    24181 cggttgccac cggtgcctct gtcggctgcg gctgtgcttc cggagcctgt gtagatgtcg
    24241 gttgcggtgc ttctgtcact gctgccgcag gctccggagt aaccgtctcg gggatttgtg
    24301 tagtctccgg ttccgccgtt gccacctttt ccgatgcgat cccggtctcc ggggtctctg
    24361 cagaagcctg ttctgagctc tgcgcttctg ttgtcaccgc cgcgaattca ttattttccg
    24421 tagttctgtc atgatggctc agtttccaca gcggaataca gatcaggacc gccagaacca
    24481 tcatgcccgc gaaagccgga atcatccatg gccggaaccg gaagccgatc ccgtccccct
    24541 cgtcgaagtc atccaggtca tccccttccg ggtactcccc atagtcctca tcctccgtaa
    24601 agtcctccga ttccacagta tacggatcct ctgtatcaca ggttgtttcc catacagcat
    24661 catcacgtcg tcccgcctgt tcagaatcca cctctgcatc cgcaagttct tcatagtcaa
    24721 aattatattc ttccggttcc cttttccggt ctttgacacc cttttcttcc atggcatacc
    24781 ctccttccgc catctttaag ctgattatat acttccctct tttgcaaaac aagtaccttt
    24841 tcctgtcttt gcaggtatgt tatactgact gtagcattat aaaaacctct caggagttat
    24901 attctctatg aaaaataaac ttgccaaatg gaaaccttat attttcctcg cggtcctgct
    24961 cgccagcctg gttcctctgg tatggctggg gcgttacaat tatcccaccg gggatgatta
    25021 ttattacggt acggaagccc atctggtctg gcagcagacc ggaagcatac cacaggccat
    25081 ttccgccgcc tgtgccggtg tcgccaaaag ctatcagatc tggcagggaa cctattcagc
    25141 gctgtttctg atgtaccttg cgcccaatgc cttctccaat accgcctatc atctggtgac
    25201 ttttgtgatc cttcttttgc tgtgcggcag cattttctat ctgctacggc ctctggtctg
    25261 ccattttctg ccgggaacat gtggggaatg gatcacgatt tcctcagtat tcagtttcct
    25321 gtgcatccag acagtggcct ttcagagcga cagcttttac tggtacaacg gctccatgta
    25381 ttacaccggt ttctttgccg tgaccctgtt tttcctgggc acattgcttc gctacctgta
    25441 caatgggaaa aagatactga tccttccgct tctcctgttc gcaattttct taggcggtgg
    25501 caattacgtg tcccttctcc cctgcatgct gtttgtggta accgtaacct ttctactgct
    25561 gttgcagaga aataaaaaga cttatgtctg cggaattacc tccgctgttc tcctgctatc
    25621 ttttgccgtc agtgccatgg cccccggcaa tcaggtgcgg caggacggca tgtggaagat
    25681 tcccgcctgg aaagccattg ccaaatgcct gttgcagggg atccgctata cctttgcctg
    25741 gaccagactg tggtggttcc ttgccgtcct gcttctgctg ccggtcttcc tgcgcatttt
    25801 acagaagaaa aactggggat ttttctccca tcccctcctt tttaccggat attcctacgg
    25861 attgttctgc tccatgtcct gtcctctgtt ctataccatg aactccacag gacccggccg
    25921 ggccgtagcc attgtctact atacgtttct gctgatcagt tttgctgtgt tcttctactg
    25981 gctcggatat atagtccgga agctgcaaat gcgtcagaat actccggaga aaatggcggt
    26041 cctcggcaag ttgaaaattg cccgttatgt cgccatgacg gtcctgttgg ccgtcatcct
    26101 tttcaccgga gtatggcagg agacctccgc cgcaaaagcg atccggatgc tgacaaacgg
    26161 tgaagccgct gcctatgctg cagaatacga agaacgtctt cttctcctga atgaccctga
    26221 gattacggat gtggtgctga ccccctttac ccatcagcct gccatgatct ataccggtga
    26281 tctccccgga gacccggagg atcccaccag taaaaagacc gcacaatact ttggaaaaaa
    26341 ttccatatac gtagattaca gcaattaatc ccccaaaaaa ggacgcttcc ggtttttctg
    26401 gaaacgtcct tttgttatct tttactattc ttattgtcct tcgtacttct cactnnnnnn
    26461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggaaac gtccttttgt tatcttttac
    26581 tattcttatt gtccttcgta cttctcactc tgcatacgga tcagttctgc atcgtcgaag
    26641 taattgatac gcatagcgct ctttacctca gacagggtct gtgctgccac ttcacgggcc
    26701 tcgtcactgc ccttcttcag gatctcatat actgcgggaa tatccttctc cagttctttt
    26761 ctacggttac ggatgggctc caaggtctcc tgcattacgt tgttcaggaa cttcttcacc
    26821 ttcacgtcac ccagaccgcc tctggtataa tgcgccttta actcgtccag atttgcgtaa
    26881 tccggcaggt atctctcgaa gtgctccggc ttgcagaaag catccaggta agtgaatacc
    26941 gtgttgccct ccagatgtcc gggatcgctg accaacagat gctcgggatc ggtgtacatg
    27001 ctcatgatct tggtgcgtac atcgtctgca ctgtctgaca gatagatgca gttgccaagg
    27061 gatttactca ttttcgcctt gccatcgatg ccgggcagtc gctgacatgc ctcattttca
    27121 ggcagcagtg ccttgggctc taccagtacc ggtgcatata cggtgttgaa tttgtgtacg
    27181 atctctctgg tctgttcgat catgggcagc tggtcaccac ctacgggaac ggtggtcgcc
    27241 ttgaaagcgg taatatcgga agcctggctg atgggatagg taaaaaatcc cacagggatg
    27301 ctggcttcga agttacgcat ctgaatctca ctctttacgg tcgggtttct ctgtaatctg
    27361 gctacggtta ccaaatccat gtaatagaag gtcagctcgc acaattcgga gatctgagac
    27421 tgaatgaaga tcgttgcctt gctgggatcc agtccgcagg acatgtagtc cagcgccact
    27481 tcaataatat tctggcgcac cttctcggga ttctcgatat tatccgtcag cgcctgtgcg
    27541 tctgcgatca tgataaaagt cttcttatat tcgccggaat tctgcagttc tacacgtctt
    27601 ctcagggagc ctacataatg tcccacatgt aatcttccgg tgggtctgtc acctgtcaaa
    27661 ataattttgt ccatttcgtc ctctcaatcc gccgtctaca cggccataca tcattgtttc
    27721 tatcatacca tctttttgca tagaagcaaa tgatttttct acagaattaa cgctttgtgt
    27781 gcaagccccc tcgcattcat tctgctgttc tatggctgaa ctattgcaag cagcttatcc
    27841 agaattttat gtgccacctg ttccttgctc atcagttcca gactctcctc ggaattcttt
    27901 gtgatcagtg tcacaatatt ggtgtccgtg ccaaagcccg ctcctgcgct cttcaaactg
    27961 ttggcacaga tcatgtcaca gtgcttgctc tccagcttct tccgggaatt ctccagcaca
    28021 ttttccgtct ccatggaaaa tccacacaaa atctgtcccg gtctcttgtt ttccccaagg
    28081 gccttcaaaa tatccttcgt ccgcaccaga gggatcgaca tgtctccgtc ttttttctta
    28141 atcttattgt ctgctgtcac tgccggggtg tagtctgcca cggctgctgc cttgatgaca
    28201 aaatcatagt cctgcgctct ggacacgatt gcatcgtaca tctctgctgc cgtctgtacc
    28261 ggcaccacat ttacaaacaa aggcggctcc acatccgtat gggctgccac caccgtcact
    28321 tctgcgccgc ggtgcattgc agctctcgcc agggcataac ccatttttcc actggagtga
    28381 ttcgtgataa aacgcaccgg gtcaatgggt tctctggtgg cacctgcgga gatcagcacc
    28441 tttttgcccg ccagatcctt ctcgcaggcg atctccttca ccacggcttc gaacagtacg
    28501 gactcctccg gcattctgcc gtcaccgata tcgccgttgg ccagcattcc cgtggccgga
    28561 gtgatgatct catagccgaa atgctttaat ttttcgatat tatcctgcac gatggggttg
    28621 tgatacatat tatgattcat ggcaggggac acaatcttct tgcagggaca tgccatgacc
    28681 gtagtggtca acatatcatc tgcgatgccg tttgcaatct tgccgatcac attggcagaa
    28741 gccggtgcca ccagaaccac atccgcccgc tttgccagtg ccacatgttc cacactgtac
    28801 tggaaattgc ggtcaaaggt gtccaccaga catttatgtc ccgtcagact ttcaaaagta
    28861 atggggttga tgaactgcgt ggcattctgt gtcatcagca cttccacatc acagtgctgt
    28921 tttaacagca tccgcgctac attggccatt ttatatgcag caatacttcc cgagattgcc
    28981 agaactacgg tctttccctt cagcatagtc gtctcctttt gcctgattta tcaaaaatac
    29041 ttcaaaactg tactcctaaa ataccataag tgcatatttt atgcaaatat cacttttctt
    29101 tttgtccggg atatgatata aacagtagga gtgtcccgaa tcaccgggac aacttgtatt
    29161 gtatctccca gagggagaat ggtaacaagg aggaaaattt catatgaaca aacaaagtaa
    29221 gacaatgcga ctcacgttga tgggattgtt tgctgcggta cttttgctga tggcttatac
    29281 cccgctcggg tacctgaaca tcggccccct ggcaatcacc ttcaatgtca tccccgttgc
    29341 actggctgca atcactcttg gccctgtagg cggagccgcc atgggagcag tattcggatt
    29401 gaccagcttt ttgcaatgca tcggcgtggg aggcaccagc gccatgggag caatgctttt
    29461 tggcatcaac cctgtgttgg ccttcatcca gcgtttcatc cccagacttc tggacggatt
    29521 tctgctggga tatgtcttcc gttacagccg caaagccgta ggagacagaa tcgcctgcat
    29581 gatcaccgga ttttgcagcg catttttcaa caccgcattt ttcatgtccc ttttagtact
    29641 gctgttcggc aatacttcct atgtgcaggg actgatgggt ggtaaaaacg cgattttatt
    29701 catctgctct tttgtaggtg tgaatgcggt cttcgagatg tgtgcatcca cgttcctgac
    29761 cggagcggta gggctggcac tgatcaaggc aaaagtcatt cctgtatatc agaaagcaag
    29821 gtctgcagca taacaggagt atacccgtgg tactgacaat cgacatcgga aatactacga
    29881 tcggcatcgg gggatttcag ggaaataagc tgctctttat tgagaagata tcgtcccgtc
    29941 gtcccctgtc tgatgccgga tatattgaaa aactgaatat gatcttgaac aaacacggcc
    30001 tgtccgccga ggcctttgag ggatctatga tttcctcggt tgtgccttcg gtgacagccg
    30061 ttttttcctc tgctctggaa acttttctgg gacggaaacc tctgattctt tcccataagc
    30121 tggatctggg ctacgagatc tgtacggaat tcccggaaaa ggtgggttgt gaccgactgg
    30181 cagatattgc aggagccatt tcccgctatt ccctgccttt cgtcaccatc gacctgggca
    30241 ccgccacgac ttttaacgtg ttggatgaga accgtcgctt tttaggtggc ctgatccttc
    30301 ccggcgttgg aaccgcccac agggcacttt taggcaagac cgcccagctc ccgaaagggg
    30361 acattcatat gcccgcccac accatcggtg cgaatacgca ggaatgcatc accgggggaa
    30421 tgctcttggg caacgcctgt gccatcgacg gactgatccg ccgtatccag acagagttag
    30481 ggcaaaaagc aacggtcgtt gccaccggag ggtacgcaac gaccgtcatt ccacattgtg
    30541 aaacgaagat ccatatcgat cagaatcttc ttatgaaagg tctgagatcc atctacgaga
    30601 ataatacctc tactggatca ccagattaga gtagcccatc aggctctcat ccacagcatg
    30661 tgcggcttct ctgccttcac ggatcgccca caccaccagg gactgtcctc tgtgcatatc
    30721 accggtgacg aatacatttt ccacactggt ctggtattgg ccgggggcag tttttacgtt
    30781 ggtgcgttca tttaattcca ccccgaaagc atcggtaata tatttctgac ttcccaaaaa
    30841 tcctgctgcg atcagtacga tctccgcatc caggatctgc tcacttccgg cgatctcctg
    30901 catcttcatg cgtccggtag ccggatcctt ttcccaggac agcttcacga tcttcacggc
    30961 tttcaggttg ccgttcttat ccttgataaa ttccttgacc gtagattcat agattctcgg
    31021 atcatgtccg tagactgcaa tggcttcttc ctgaccgtaa tcggtcttgc agaccttcgg
    31081 ccattcgggc cagggattgt tctctgctct cttgtccgga gccttgggca tcatctcgat
    31141 ctgggtcaca gacttggcac cgtgacggat ggaagttccc acacagtcat taccggtatc
    31201 accaccgccg atgatgacca cgttcttacc cttggtctcc acatattttt tatcttcaaa
    31261 gttcgaatcc agcaggctct tggtattggc tttcagaaaa tccaccgcaa aatagattcc
    31321 tctggcatct ctgccgggag cattgatgtc tctaggattg gaagcaccac aggccagtac
    31381 gacacggtca aaatcatgta acagcttgga tgccttgatg tccttaccga tatccgtacc
    31441 ggtgacaaag gtgacgccct ctttcttcat gatggctatc ttacggtcaa tgatctgttt
    31501 ctccagcttc atgttgggga tgccgtacat taacagtcct cctacacggt cggagcgctc
    31561 aaatactgtg acctgatgtc ctctctggtt cagcagatcc gccgctgcca gtccggaagg
    31621 accgctgccg atgactgcta cctttttgcc ggtacgcacc acagggggct tggctgccgc
    31681 ataaccctcg ctgtaggcgt tttcaataat tgcatattcg ttctccttgg tggagaccgg
    31741 atccccgttc aacccgcagg tacatgccgc ttcacacaag gcagggcata ctctggaagt
    31801 gaactcgggg aaattgttgg tcttcttcag gcggttgtaa gcctgctgcc agttgccggt
    31861 atataccagg tcgttccact ccggcaccag attgtgcagg gggcatccgc tggccatacc
    31921 gcagatcatc atgcccgact ggcagaacgg cacgccgcat tccatacatc tggcaccctg
    31981 taagcgctgc ttttccttcg gcagatgctc atggaattcg ttaaaatgct gtattctctt
    32041 cttaggatcc tcagccacgc tgacttccct tttatattcc ataaatcccg ttggttttcc
    32101 catgactctc tccattctgc agacgcttcg gcgtcggaag aatcgcgaac taaatttcat
    32161 atttagttct gcgataaact aatttgtggc gcccgcggca caaattagtc gtgtgaaaag
    32221 caatctatca agattacttt tcacactggc ctcctacttt ctggtgttgg catagaatgc
    32281 ttcgatctgt gcctgctctg cgctcagtcc tttttcctcc atctggacga tggtctttaa
    32341 gactctctca taatcatgag ggatgatctt cttgaacttg ggcagatact ctccaaagtt
    32401 gtccagcact tccttaccct tggcggaatt ggtcagtgcc acatgctcct ggatcatgga
    32461 tttcaactcc agcacatcat atttggatgt gatctcgctg gaggatacca tttccttatt
    32521 cagacgcttg tacagatcgt tgccttcatc cagtacataa gcgataccgc cgctcatacc
    32581 ggctgcaaag tttttaccgg tgggtcctaa gactaccaca cgtccgccgg tcatgtattc
    32641 acaaccatga tcacctacac cttcgacaac tgcgatggca cctgagttac gtacgcagaa
    32701 acgttcgcct gccacaccgt taatgaatgc tttaccggag gttgcaccgt acagtgccac
    32761 attaccgatg atgatatttt cctcagcctt gaacttgctg ccctgaggag gatataccac
    32821 cagcttaccg ccggataagc ccttaccgaa gtagtcattg ctgtcgccta ccagttccag
    32881 agtcaggccc ttggggataa aggctccgaa ggactgtccg ccggcaccct tgcacagtac
    32941 atggtaggta tcctcttcca catcatcgcc caattttctg gtgatctcac ttccgaggat
    33001 cgtaccgaaa gcacgatcgg tgttgctgac gtctacttcg atgctgcgct tctgtcccgc
    33061 atcaatagct ttgcccagtt gctttaacag caccttctca tccagcgtct tctccagctg
    33121 gaagtcatag acctgcttgg gatcgaaggt caccttctgc ttgctgcctt cgtaagggtt
    33181 gcttaagatc tgggtcagat ccacttcctc ctgacgatcg gtcagatgct cttttctctt
    33241 cagcagatcg gttctgccca ccagttcatc tacagtacgc acacccagtg ctgccatgta
    33301 ttcccgcagc tcctgtgcga taaatttcat aaagttttcc acatattccg gcttaccgcg
    33361 gaagtttttc cgcagctccg ggttctgggt agccacgcct accgggcagg tatccagatt
    33421 acatactctc atcatgacac agcccatggt cacaaggggt gcggtggcaa aaccgaactc
    33481 ctcggcacct aaaatggctg cgatggctac gtctctgccg ctcatcagct taccgtcggt
    33541 ctcaatgcgg actttattac gcaggccgtt catgatcagg gtctgatggg tctctgccag
    33601 tcccagttcc cagggaagtc cggcattgtg gatggagttt ctcggtgccg caccggtacc
    33661 gccgtcatag ccggatacca ggatgacctg ggctcccgcc ttggctacac cggctgcgac
    33721 cgttcccaca ccggcttcgg ataccagttt tacagagatt cttgcctggg tgttggcatt
    33781 tttcagatca tagatcagct gcgccagatc ctcaatggaa taaatgtcgt gatgaggggg
    33841 cggagagatc agggacacac cgggcgtgga gtgtctggtc ttggcgatcc agggatatac
    33901 ctttcctccg ggcagatgtc cgccctcacc gggttttgct ccctgtgcca tcttgatctg
    33961 aatctcctgt gcacttacca ggtaacggct ggtaacacca aaacgtccac tggctacctg
    34021 cttgatggcg gaacaacggt tcagtccgtc cggtccgacg gtcagacgat ccaggtcctc
    34081 accgccttca ccggaattgc tcttgccgtg cagatggttc atggcgatgg ccatggtctc
    34141 atgggcttct ttggaaatgg aaccgtagga catggcaccg gtcttgaatc tggtcacaat
    34201 ggactctacc ggctctactt cctcaatagg cacacccttt ttcggataat tgaagtccat
    34261 cagtcctctg agattcttga tggaatcttc gtcgtttacc atggcagtgt actgtttgaa
    34321 catctggtaa tcaccgcgtc tggtggattc ctgtaacata tggatggtag cgggattgta
    34381 cagatgctcg tcaccgccgc tgcgcatctt gtgatgtccg gggctgttta aggtcagatc
    34441 gtttttcagt cccagaggat caaatgcctg agagtgcagg tcgtctacct gctgctcaat
    34501 atcttttaag gtaatacctc ccacacgaca tacggtgttg gagaaatact tactgatcac
    34561 ttccggtgca ataccgatag cctcgaagat ctgggaaccc tgataggact ggatggtgct
    34621 gatacccatc ttggatgcga tcttgacgat gccgtggagg acagcattgt tgtaatcatc
    34681 cactgctgca tagtaatcct tatccagcat attgctctcg atcagttcct tgatggtatc
    34741 ctgtgccaga taagggttga tggcgcaggc accgtaacct agcagcgtag caaaatggtg
    34801 tacctctctg ggttcgccgg attccaggat cagtgcgact ctggttctct tcttggtgcg
    34861 taccagatac tggttcaggg cggatacggc cagcagggaa ggaatgggca catggttctc
    34921 atccacgcct ctgtcggaca ggatcaggat gttcacgccg tctctgtaag ctctgtccac
    34981 ttccacgaac agtctgtcaa tggcacgctc taagctggtg ttcttgtaat atacgatagg
    35041 tacgacttct actttgaatc cgtctacctt catgttcttg atcttcaaca gatccgtgtt
    35101 ggtcaagatc gggttgttga ccttcagaac attacagttc ttcggggtct cctccagaat
    35161 gttgccctcg gtaccgatgt atacggtggt ggaagtcacg atctcctcac ggatcgcatc
    35221 gatgggcggg ttggttacct gtgcaaacag ctgcttgaaa taatgaaaca gcgggtggtt
    35281 cgtcttgctt aagacgggaa gcggcgtatc cacacccatg gcggcaatcg cttcactgcc
    35341 gttctttgcc ataggcagaa tgctctgttt catctcatca taggtataac cgaaagcttt
    35401 ctgcatccgc tgacgctctt cgtaggtgta ctcagggaca cgctggttcg ggatgtgaat
    35461 actcttcaat tctgtcaggt tgttgtccag ccattcacca tagggctgtc tggaagcata
    35521 tttttccttg atctcctcat cgtcaatgac tctgccctgt acggtatcga ccaggagcat
    35581 cttgccggga cgaagtcttt cctttaactt gatcttcgtg ggatcaacag gaaggacacc
    35641 tacctcggaa gacaggatca gggtatcatc gtcagtgatc agataacggg aaggacgcag
    35701 gccgtttctg tccagtaccg cacccatgat atctccgtcg gagaacagga tggatgcggg
    35761 gccgtcccag ggctccatca tggtagcgta atactggtag aaatctctct tggtctggga
    35821 catggccttg ttgttcgccc agggctcggg gatagcgatc atgaccgcca gaggcagttc
    35881 catgccgctc attaccagga attccagcgt gttgtccagc atagcggagt cggaaccggt
    35941 cttgctgatg gcaggcagta ctttgtgcag ctggcctttc agatgctcgg attccatgtt
    36001 ctcctccctg gccagcatct tgtcggcatt gccgcggata gtgttaatct caccgttgtg
    36061 taccatcaga cggttcggat gagcacgttc ccagctggga ttggtattgg tggagaaacg
    36121 ggagtgtacc atggcaatgg cggattcgta atcttcactc tgcaaatctg cgaagaaggt
    36181 acgcagctgg cctaccagga acattccttt atatacgatc gttctgctgg aaagagaaac
    36241 tacataggta tcatcggaag actgctcgaa gacacgtctt gcaatataaa gtctgcggtc
    36301 aaagggcagt cctttttcca catcggcagg ctttttcacg aatccctgca tgatataggg
    36361 catgcactct acggccttat gtcccagcac attggggaag gtaggcactt cacgccagcc
    36421 gaggaattcc attccttctt tttccacaat gatctcgaac atcttcttgg cttgattgcg
    36481 gcgcagctct tcctgcggga agaagaacat gccgacaccg tattctctct ctccgccgat
    36541 atccatgccc agagcctttg tgactcttgc aaaaaatttg tgggggatct gggtcagaat
    36601 accgacaccg tcaccggtct tgccttccgc atccttaccg gctctgtgct ccagattctc
    36661 tacgatcttc agcgcattct ctacggtcgc gcggctcttc tctccgtgaa tattgacaca
    36721 ggctccgata ccgcagttat catgctcaaa ttcttcccga tataacggtg ccttgggcag
    36781 ctcctgtttt acatcaaaca tatagttctc ctttccggtt ttctatttct ctcttcttaa
    36841 tctgtctgtt cctattcaga acctcattcg caagactgtg ctttcagcac tttcctgcaa
    36901 ctaaaaaagg tcgcaatgag actctttcga gcgcctcatt gcgacctttt ctgtactata
    36961 cacctatttt tctcatttgt aaatatagat ttttccttga attgtcagat aatttgtcaa
    37021 ttcagttatt cacacggctg ttgtctgtga attgttctaa ttttcttttt taagccatgc
    37081 ttttagcaca actctttggg gattttccct atttttcctg tctccgacca tacactttgc
    37141 ttgccagtcc ctgatagatt gcagagaaat gtctgctttc tgccattgcg gtccggagag
    37201 gtgccagggt cagtgccatc acagcgcggt aacggaacag ttctacgggt ttcatgtatt
    37261 ttgccgtgat ctccttatgc tctgcggcac tgcgtttcag gttctccttt gtctcggagt
    37321 agccgccgcc ttcgtaggaa gatatggtaa gccccagata ctgcatggga gccttcgccc
    37381 ggtaatagca ccacagaaaa tgttcataat ctgcgcggat cttgtatttt ggttcgtagg
    37441 gcttctgtcg gcacaggtct gcggcataga agcaggactg atggcaggga atgttgcggt
    37501 agcagacaaa gccggtgatt cggggcgggg atgatatctt cgtgtccgtt ttcgcgctgt
    37561 aggtatctcc gtaatagatg ccttgcagtt tcatagggtg ctgttccatg aattgtgcgg
    37621 tgtgtgccag aacttccttg tcatagaaga ggtcaccgca gtttaagaac agaatgtaat
    37681 ctccggtcac atgagagacc gcctggttca tagcctcgta gatgctgtga tccggttcct
    37741 ggtagagatg gatccgcccg tcagtgcgca tggactctat ggatccgtca cggctgccac
    37801 cgtccttgac gatgacttcg aagtctgggc aggtctgggc caggatactg tcaagggtct
    37861 gattcagttt ggtgccgggg ttcaggcata ctacgataat gctgaatttc atatgttgct
    37921 ccttttataa tgtctcatga tatttcatta tgcctcgcag tctcaaacag ggtaaggaac
    37981 gcggcacgtt ctcactctgc gcagagcgta acggtacata agtgaccaaa gtacggtcac
    38041 taagtgccga cgtctgctta cggcctgctt atccttaccc tgttttcggc tgctgggtta
    38101 tgaagtccag ttcaatacag tttatgaatg cggtcagaac tggtaactaa tgtgttttgt
    38161 tatctcatta aaatatattt ttctgtttgt gcaaagtgat caaggataca actgccactg
    38221 ccaggaatac cacgcacacg ggccagctga cgaagccggt gacggcgatg gcggtgtagg
    38281 acaggcagta ggcgaggaca ttggatgcca catgaacgag cactgccatt ttgaattctc
    38341 cgaaatactc ataagcgtag gccatgaggc agccaatgag aaagccgtag atgccctgca
    38401 cgtagttcat gtggtacaca ccgaagatca gtgcggacag agctatagca agcttcagat
    38461 ccataaatct gcgcaggtaa ccataaatca ctccacggaa cagtaattcc tccgcaatcg
    38521 gagagataaa gccgaatacc agaataccca caataaagtg tgcagaatac tgctgtgaaa
    38581 cgacttccgt ataggcttcg gatttattgg tcacacccag aagttcaaac aggaggttga
    38641 aaccgataac ggcaccgatg gtggctgcca ccagcagggc atattttgcc ttaccttctt
    38701 ttttcagatg gcttaaatgc atatcctctt tgttcttcgt gatcagaagc tttgccgtgg
    38761 accataccgc cagtgctccg ccgatgaagc ccagtgccgc tatgatactg gacacatctc
    38821 cggaatagcc gttatatgca ccggtctcat ccctcaggat cagcagatct accagtccac
    38881 cggaagcacc ggactgtgcc agctgataca acagtgccag cagcgcatac tgcaccgcat
    38941 tgcgcaccag gataaacatc aggaacgaat acaccatggt ccagattctc tggtacatgg
    39001 gatttttctt tttattgtcg ggattgctgt tcaccagttc ctcgctgccg cgaagcagaa
    39061 atttttccag ttcttccacg gattgtacga tgtagtccgc ttttgcttcc tttaactctt
    39121 ccttgcttcc gtagccataa gtcacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39241 nnnnnggctc cagcttgtcc agatcaggtt cctcaatacc gaaagagtgc agcgcatact
    39301 gcacgcaggt cgtaattcca attttgggat ctgtcagtgt accgtccaga tcaaacagca
    39361 gatatttttt cattcttata attccttttt ttgtatctgt tccaagccct gtaactatcg
    39421 caaaatattc attgaatacc gggatccgca agaatattct gtaattgtta tgaggctctg
    39481 aatagttacc ctttctttct atagcttgtg gggtccgcaa agcattagaa gctgcggatc
    39541 ttgtccccgt catcctcagt gtttttcagt gccttgatct ctacgtcata gcccacattg
    39601 gcattgacct gcacatggac cacatcaccc acttttttgc caagcagtgc cttgcccagc
    39661 ggagattcgt tgctgatcag accttccagt gagtttccgc gaacggtggt cacgatcttg
    39721 taatcctctt ccgtgccgtc gtcgatgaat tccacggtaa cagtgttgtt gatgcccacc
    39781 tcatccacgg cggagttctc ggagatcacc tttgcggtct tgatcattct ctccagataa
    39841 cgaatccggc tctcgttctg attcttgtat tttttcgctg cataatattc aaaattctca
    39901 ctgagatctc cgtgggctct ggcttccttc acgtcctcca gtgccttggg acgcaccacc
    39961 agcttacgat actcgatctc atcttccatt ttcttaatgt cagtgggggt taattcatcg
    40021 tacataattt ctccatttct atctgttcaa ttacttatat catttattga cagtttttct
    40081 cccggctcgg cacatgctga ttttctacca acagccgtcc ttctttttct tcggcgaccg
    40141 accttctttt ccttcagcag acgcctttct tttcttctga cggccgacct ttttccttcc
    40201 ggcagccaac cttcttttct tgcagaggat gaccggtcgc agtctctatg ctgtcccggt
    40261 gtgcccagca ttctacaact gcattgcgta gattaccgcc agagacagaa tgatctgaat
    40321 aatggcaaaa cggaatacag tctgtgtatt ggaccagtcc cacaccttac gcacatgatc
    40381 atgcaaggga gtacgcacat ttttcaaaat atgaatcttc aggaagcgga gcagggaaac
    40441 cttaagcagt cccaaacctc cgtccagaat cagcaccaga gccagcggaa taaacaaaaa
    40501 cggacagccg ctctttaaaa tagcgatgct gatgaacagt cccatggccc gggaacccgc
    40561 atctcccatc atcagcttac tgggtgttgc attgtaccac agataaccca gaatgcatac
    40621 ggcaaacagc aggatcagga aagaataggt ctcattgacg tccttcatac ggtccacgat
    40681 atagatggac atgatcgtaa tgatcgagag ggttccggag agtccgtcca caccatcgga
    40741 gcagttggtc acgttcacgg aggtccatac caggatcacc gtgagaatgg cgaataccac
    40801 aggcggcagg gtgaacttta catgaaacag tgccagctcc accacgttgg aattatattt
    40861 caggaacgtg atggctacca gtgccgccac gcacagatct aagaaacctt ttttgtactc
    40921 accccatgga gtcttggaag catcgtccag aaatcccgtc atcatgcata ccacgatcag
    40981 cagcagatag atcaccagct ctgcgcttaa aggggaaaac agcagaacgg atgccacaaa
    41041 tgccatgaca aaaataattc cggcacctct gggtttcccc gcagacaatt ttccgtcatg
    41101 ggcaaattct cttcctgcat ccttgggcag atacggactt aatttcgccg ttgccagtac
    41161 cgtcgctcca aaagcaaaaa ggattccaaa aaaagcaagg agagaactgc ttccctccgg
    41221 caagatcatc tgtaacatac tatccctcgt ttccacatga gatactcact tttgtactct
    41281 catgctgtat ttttctgata aattccgtaa ctgttcacag gggcattcga gaaatgaaca
    41341 cctcagaaca gtcacaaaat attttaacac aaatatcccg gaatctcaaa taatttccac
    41401 aacagttcag ttctgaacat atctgggcgc agaaatcatg cttgcatgat tttgcggaca
    41461 aatatgtgca tgagctaagc gccaatctat gagcaaaggt agtcacgaag tgacataaaa
    41521 gcgtgttaac gcgactcccg tgaacggcgc ggctgaactg ttacgtttga cagtttcccc
    41581 acggtggact actatattct tatgaacact ttctgaaaaa tagccataaa aggagaaata
    41641 ctctatgatg atccataaga tcgaaccgca taaatattac ccggaattcc gaagccagga
    41701 gcctaaacct tccgattatc tgctgttatt tgacggagaa aaggtctatc tgccctccca
    41761 tacagaaaac agtgatgtaa cggatacttc cgccgatgcc ggcatctcgg agcatcctca
    41821 agtatatctt cctacttttg aagaagtcca tggactcttg ggaatccgtc agcctatgga
    41881 acgttttttt ctggactgtg aatatctctt ttccattgat gaccgggcat tcttccgcaa
    41941 gacgacggag gatgccggtg ctctcatgct cccggaagaa gacctctacg cttccggtgt
    42001 gttccgtaat tttgagccgg aatatctggc atttgctgga atcactgcca cccagatcaa
    42061 tcgtttccgt ctggaccgga aattctgcgg ccgctgcggg catcccacag ttcccagcac
    42121 tacggaacgg gcctgcatct gtccggagtg cggtcagatc gaatatccca agatctctcc
    42181 agccgtcatt attgccatcg tggataccga gcgggacaag atcctgctga cccgttacgc
    42241 cggcggttcc tatcgtcact gggcactggt ggccggcttc gtggaagtcg gtgagacctt
    42301 caagggtgct gcccgccggg aaatcatgga agaagtcggt ttaaaggtca gcgatctggt
    42361 ctattacaaa tcccagccct ggtccttcag cgattccgcc atgatcggct tcttcgcgaa
    42421 gctggagggg gatccttcta ttactttgca agaatcggag cttggcgagg ctgcctggtt
    42481 ctccagagag aatgtcccgg atctgccctc cactatcagt gtcggtcaag aaatgatcac
    42541 attattcaag aatggtgggg atccgttctg ccagagataa ttgatacatt tgaaagtttt
    42601 tcacttctat tccatgatat accttgactt tgtgcccccg tggtttcatg ccccgggggc
    42661 ttatttagtt tcttttcctc tctgcgcccc gctctctcga tctccccggg gcttannnnn
    42721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttatt tagtttcttt tcctctctgc
    42841 gccccgctct ctcgatctcc ccggggctta atcagcatgc tccttatatc tacgccccat
    42901 gtacgcaaaa atctgccttc gacaataaca ttgtcatgag gcagactcct ttctgtactt
    42961 attctcttca ctgtttcttc acctagagca tcgtagctgt catcgcttct cattatctca
    43021 ataagcggca cttcttcgct accttcacta acagatatcc cttcgggaag ctgcgaagct
    43081 cctgctccgt gatgccccgc atcaatatca gcatgacaaa atagactaca gcagcaatta
    43141 cgatcgcagg aatgacagag atccgcatgg acttcgtcac catatacata ccctcgtaga
    43201 ttgcccaggc tgccgctccc ataaacgcgg aggcgaacgc cggaatgacg aaagtcttta
    43261 cgatctcctg ccggtagcct accgctcttc ttaccgccca ctggttcagc acacacatca
    43321 taccggaata cagcgtattg gcgatggcaa tgctgtacag atccagtctt gtgaacatca
    43381 gaagcagcac tgctgccacc gtctgcacca ccagcgagat ccccgcattg atgatcgggg
    43441 tattgacttt gccaagtccc tgcaaaatcg aactgttcag agtcgacagt gcataaaaga
    43501 taaccgacag tgacaatgtc attaaaagag ctgccgccag attcaattcc tctgccgtct
    43561 tattcgtgaa cagcagactg atcaccggct tcgccagtac aaaaagtccc acagcgcagg
    43621 gaatcgagat caccatggta gtcttcactg ccagtccgat cttctctctg gctcccttca
    43681 catcccgggc tgccatcagc tgtgctacgg aagggatcat ggcagaagcc atagcagatg
    43741 caaaggctat gggaatattg gacattgtca gggactgtcc ggagaaaata ccatattttt
    43801 cataaataga taccgtatcc agattgcgaa gggacggata ccatttggtg tagatactgt
    43861 tgttgaccgt actgcttaag ttatatacag ccgtactcaa aataaacggt gtcaccacca
    43921 gtgtgatcgt cttgaatatc tgtccgtagg aatccacatg ttcatgcctg tcacgctgca
    43981 ctctgcgcag gatcattcca cgattcagag catagactcc cgccataaaa agcagcgcca
    44041 ctaatacgcc gacaccggta cccagtgcac ttcccacggc accgtaggtt gctctcatca
    44101 cctgccctgc ctgatccgcc ggaacctcca tcgttcccat aaacatttgg atcagaaggt
    44161 atgccgcgcc tacgctgact accgcattgg cgatctgctc caggatctgc gatacggagg
    44221 tctgcgccat gctcttatgt gcctgaaaat accctcgtaa aactcccagg ataccataga
    44281 cgaagatcgt cggtgcaaag gtgcgcagta ctgtgaccgc tgctccggac acgaataatc
    44341 ccgctccgaa aaacagaaaa aggctggcta taccgccaac caccagtaca tagatcatgg
    44401 ctccgtggaa cagtctgtgg gcgttacggt attccttctc tcccagcttc tgtgcgatag
    44461 ctttggaaat tgccgaggga atactataag aggagatcaa cagaatgatc gtataatagt
    44521 tatacgctga ctggtaataa ccaagtccca gattcccgat gacactctgt aaagggcccc
    44581 tgtaaaggag ccctatgatt ctgctgatga ttccggctgc cgccagaatt cccgcctgca
    44641 tgataaaatt gttcttggaa ctgttcttat ctgccatatg taatcttacc ttattccagt
    44701 tctttatccg atcagccagt catacatctc ctggtatttt ccggcggata ccttccagga
    44761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taacgttcct caccggtgcc
    44881 cagtaccacg aactggatat catcctgaca cagttcatcc atgacataag cgatcagatc
    44941 cagacccttc tggtctgtca gtctggatac cagaccgatc atcattttct tatcatctac
    45001 gcgaagtcca agctcttcct gcagggctcg cttattcttc accttttcct tgcggaaatt
    45061 cactgcatta taggctttta cgatattttt gtccgtctcc ggattgaact ctccatagtc
    45121 gatgccgttg acaataccac gcagatcatg acttctcgca cgcagcaatc cgtccaggcc
    45181 ctcaccgtaa aacggagtct tgatctcctc cgcataggta tcacttacgg tggtcactgc
    45241 atccgcgaat acgataccgc cctttaacag atttgcatcc ttataagcct ccagcttgtc
    45301 agaggtaaaa taatactccg gcaatccggt aatgctctgt accgtcttca catcccattt
    45361 tccctggaac ttcagattat gtatggtgat cacagacttc atgttgcggt agaaatctcc
    45421 gccatggaaa cgctccttca ggtataccgg gatcagaccg gtctgccagt catggcaatg
    45481 gatcagatcc ggctggaaat tcaaaagcgg taatgccgac agcactgcct tgcagaaata
    45541 tgcatacttc tcgatctcat atctgggatc atccccataa ggcttgaaac caccgaaata
    45601 actctcatta tcaatgaaat aataatgcac cccggcttcc tctgcctcca gaacacctac
    45661 gtacacattc tgccagttga aatccatgta gaaatgtgtc acatacctga gtttatcctt
    45721 catctcctgt ttcatacagg tatacttcgg gataaacact cttacatcaa aatactcctt
    45781 gtcaatatac ttcggcaaag atcctacgac atccgcaagt cctccggtct tgataaaagg
    45841 tacgccttcc gatgcggcaa aaagaatttt tttcatgtaa accctccaca tatgctacgg
    45901 cttgtattca aaatatagat tgattatacc atgtttacga ttcatagaaa agag
//
LOCUS       DAWEJW010000007        45407 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_389_length_45407_cov_51.463045, whole genome shotgun sequence.
ACCESSION   DAWEJW010000007 DAWEJW010000000
VERSION     DAWEJW010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 45407)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 45407)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..45407
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_389_length_45407_cov_51.463045"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT        12639 a         9330 c        12163 g        11275 t
ORIGIN      
        1 atgagcggtc ggggtctggg acacaccggt ggaaccattg ataagctgga aagtttcccc
       61 ggattttcta cttcactgac tacggaacag tttattgaaa atgtgaacaa aatcggtatt
      121 gccattatgg gacagaccgc agatctggca ccggcggata aaaagctgta cgccctgcgg
      181 gatgtgactg ctacggtaga caatatgtcg ctgatcgcca gctccattat gagtaagaaa
      241 ctggcagccg gtgcagatgc cattgtactg gatgtgaaga ccggaagcgg tgcttttatg
      301 aagagcaggg aggatgcttt tgctctggcg gaggaaatgg tgaagatcgg taagaatgcc
      361 ggacgtaaga ccattgccgt gatttcggat atggatcagc cgctgggccg ggccgtggga
      421 aatgccctgg aagtgaagga agccattgca accctgcggg gagagggccc ggaagatttc
      481 aaagaacttt gtatggtact gggaagtcgg atgttacttg ccggtagtaa ggcggagagt
      541 gtcgaagagg ccagaaatat gcttacaaat gtcatcagag atggcagtgc tttacggaag
      601 ctggagcagt ttgtggaggc ccagggcggt gaccctgcgg cggtaacgga tccggatctt
      661 ctgccgaagg catccatggt ggttcctgtc tgtgcggaca gagacggctt tgtagaaaaa
      721 attgcctgtg aggatatcgg aatttgttct ctgctcttag gcggcggaag agagaccaag
      781 gaaagtgaga tcgacctgtc tgttggcatc ctcttagaga agaaaaaagg ggatgcggtg
      841 aaaaagggag aggtactggg ataccttcat gccaatgatg agaaacgcct ggaagcggcg
      901 aaggaaaagt tccggagtgc ttatttcatc accggccatc agccggaggc agaacctctg
      961 atcaaaggga ttttacagta acatattccg gcatccgttt tcatatagtt ttatatgaaa
     1021 atgaagagaa tgccatcgaa aaaagaaaaa tggatcgatt ccctgcagtt acccaaggat
     1081 gtctgtatgg gagcactgcg ggtgacgctg acgggaaact ccgaagcctg gattgaaaat
     1141 tacagaggaa tcctggaata taccggagag cggatccttt tgcaggccaa gacctgtcag
     1201 gtgtgcctgg agggaaccag actttccatc gactattata ccaatgaaga tatgaagatc
     1261 agcggaaata tcagtgcact gcgatatttg cgtgaataat catttggatg agaggttaac
     1321 aggatgacgg aatgggtcaa acgtctgcag ggttatgtga agatccgggt atggggcttt
     1381 gcaccgcagc gatttatcaa tctgtgcagt aataagggaa tattgctgtg gaatatagaa
     1441 aaacaggaag atgtttatac catgtgcatc ggcctgcggt ctttttacga actgcggccc
     1501 atcgccagaa agacaaaagt aagggtagtc atatcggaaa gatatggact accctttttt
     1561 gtgccgggaa tgctgcgcag aaaagcgttt ctggcaggtt tatttctggc ggtggctttc
     1621 tggctgattt cgtctttatt tgtgtgggat attcaggtga ctggcaatta tcaggtgacg
     1681 gatgacgtgt tctattcctt cctggagcag gagggcattc acaccggaat gcgaaggagt
     1741 gaactgaata taggtgagct ggaaaaacag ataagacgga agttttcgca ggttacctgg
     1801 acctccggcc gtctggacgg gaccagactg gtgatcgaag taaaggaaaa cgatatgtcg
     1861 gttccagaag tcgtgcagga tgctgcaggg gaaggaaaag atctggtggc agaatgcaac
     1921 ggggtgatca cggatatgat cgtgcgttgt ggtgtcccga aagtatccat aggcagtgaa
     1981 gtggcaaaag gagatattct ggtggaaggc aggatcccca tttatgccga ggatggaacc
     2041 gtcagggaat atcggccggt gaccccggat gcggatatag tgctggagca taccggaacc
     2101 tttcaggcat atcttccggc agattatatc cggaaaaaat acaccggaag acaaaagaca
     2161 ttttatttta tccatttcgg tgaccgggag tggaaaccgg agaaaaaaat ggattttttg
     2221 caatatgaca gcgtgctgga agcatgtccc gtaaaagcgt tggagtatct gcagatcccc
     2281 tgcatggtag gcaggatcac gtacagagag tatcagaaaa cagaatatta ttatacagtg
     2341 tcggaagcag aaacggtttt acagaaaaaa atcatggatt ttcttgaaac tttagatgaa
     2401 aaaggggtac aaataatctc gaaagatgtt aaaatagaaa caaagcgcgc cggctggacc
     2461 gcacacgggg aacttttgct gcgggaagcg gcggtcagtc tggtagattc ccgggcggag
     2521 gataccggag cagaagattt ggacagaaat gaggattaag acataaatga gtgtgacagc
     2581 aatccgtctg gaaatggata cagaacagat gcagaagatc ttcggtatgc aggatgcgta
     2641 catcaagaag ctggaacagg attttcaggt aactattgtg gaccgcaacg gaagtgtaat
     2701 gatcaccggg gaagaggaaa acgtttcgaa ggcctcccgg gtattacgac agctgaccgc
     2761 attgtcggat cacggcaatg agatcgaaga acagagcgtg gattatgcca ttgaaatggg
     2821 aaaagaagac caggaggaaa aactgatgga gatggatgcg gactgcatct gccataccat
     2881 cagcggaaag ccgatcaagc ccaagaccct ggggcagaag gcttatgtgg atgccatccg
     2941 gaaaaacatg atcgtattcg gactgggacc ggccggtacc ggtaagacgt atctggccat
     3001 ggccatggcg atcaccgctt ttaagaacaa cgaggtgagc cgtatcatcc tgaccagacc
     3061 ggccattgaa gccggagaaa agcttggctt tttgcccggt gatctgcaga gtaaggtaga
     3121 cccgtatctg cgtcccctgt acgatgccct gtatcagatc atgggagcgg aaagttttgc
     3181 caaaaatatg gaaaagggtc tgatcgaagt ggcaccgctt gcctatatga gaggccggac
     3241 actggacaat gcctatatta ttctggatga agcacagaat accacaccgg cacagatgaa
     3301 aatgttcctg acccgtatcg gtttcggctc caaagtgatc gtaacgggtg actcctccca
     3361 gaaggatctt cccgtgggag caaaatccgg tctggatgta gcagccagag ttctgaaaaa
     3421 tatcgatgat attgccttct gtaccttgac cagcaaagat gtggtaagac atccgctggt
     3481 acagaagatc gtaaaagctt acgatgacta tgaagcccgg acgatcaaaa gcaaaagaag
     3541 tccgagatta aacaaacgtc cggataaagg aaacagataa atatgaagaa caaagagaaa
     3601 aacggatttt cgcggctttt gctgccggag atgcttacgg tactgatcgg aggtgccgca
     3661 gtctacggac tgggactttt gggaaaacag ttgtctgtgg aaaatgccct gcgggatgcc
     3721 gtgatggcag cactgggact tgcagtgtca ggatttttcc tgcgcaggga agtagtggat
     3781 tcccgcctgg attatgataa cggagagcac ctgatgagat tctggaccgc tgtctggtgc
     3841 agccttttgt tttcgctggc ctgtgcattc cttccggcgg gaggatggcc gtttctggcg
     3901 gtctttgtgg tattgtccct gttcagcaat ctatcagtgg gaattgtgtt ttccggggtg
     3961 tttctgatga ttgccacgct gtggggacag tcagtgggca tttttttcct gtattttatc
     4021 agcggtgttt ttgcagcctg tcttttccag catttagagc aggaatttgc catcgggatc
     4081 cccctgttct tatctctgtt ctgccttttg ctgtgcgaga ccgcaaatgt ggtactgctt
     4141 gccaatgaac atttaagtct ggaacagttt ctggttccgg cagcaaatct gatcgtgtcg
     4201 ggaatactgc ttctgggtat tttgaaaatt ttttcgggca cagtggtgtt cagggatcgt
     4261 gtaaaatatc tggaactgaa tgacaccgag aatcaggtac tggtaaagta ccgcgaggaa
     4321 gaccgctccg aatatttcct gtgtgtacat acagcctatt tctgtgagcg gatcgcaaat
     4381 aagctggaac tggacaggga tgccctgaaa tgtgccggat tatatcacag aaaaggctgg
     4441 gatcttatgc aggagacacc ggatatggaa ttcccgtcag gcgcatcgga gatcctggaa
     4501 gaatataaag gaacccggaa atacaaaaaa gcggagaccg cagtgctgta ttgttcggat
     4561 gcggtagtca gtgcgatcct tttgctgttg cagaaggaac cggagaagaa gccggattat
     4621 gagcaggtga ttgacaggat ttttgagaga atccgggaaa aaggtatttt cagcgaatgt
     4681 gacctgtcct taaggggctg gaatcggatg cagaagatat tcaaggagga gaaattatac
     4741 tatgactttt tacgttgaaa atgaaaccga tgagacattt tcaaatgggg aaaacagttt
     4801 ccccatagag gagaccgtgg agcaggtagc cacggcggtt ctggatatgg agggatgtcc
     4861 ctatgagatc cagttaaatg tactgttgac agacaatgac ggtattcatg cttacaataa
     4921 ggaataccgg aagattgacc gggagactga tgtattgtcc ttccccaatc tggatttcga
     4981 tgagcctggg gattttgaga tctctgagga cagagaggca gattattttg atccggatac
     5041 aggagaactg atcctcgggg atatcattat ttctgtggac cgggtcaaag agcaggcgga
     5101 aagctatgga cacagccaga agagagagtt cgcatttctg gtcgctcaca gtatgcttca
     5161 tctgtgtggc tatgaccata tggaagaaga tgaggcaaag gtaatggagg caaagcagga
     5221 gcaggtcctc gcatcccttg gaattacaag agattaaaaa gggcgtgtca tacacgtaaa
     5281 ggtagcttac atgaatcagg tggaagtaaa aagaagacaa atacagagtt tttcctatgt
     5341 ccttgcctgt atcaatatct gggtgttcgg gaaaagcatc ggagataacg gcctgggata
     5401 tctggcagcg gctgttctgg tatttgccct gttctgggtg cttaccggga aaaatctgcc
     5461 ggacagaatg ggcagaatgc ttcggggcag aaatgccagg gggcagtatc ggaatgtctc
     5521 cagaatgcgt aagaacatga tgctgttcca gatcgcagag ggacttcttg ggactgtttt
     5581 gtgcatgagt ctgggctgga tccttttgga aaaggtcttt ctggtgcctt acggaagcat
     5641 gatcctgtgg atcttaagcc cggcactttt tcttcgttgt atccagtcgg tatttttagg
     5701 atattttcag agtgaaggtt cggaaatgcc atctgcggta tcttcggtgc tgcggcaggt
     5761 atttttcctg ggcctgggtc tggtgttcct ggggattttc cggaactacg gggagaaagt
     5821 aagtctttta ttaaaaagga cagactttac atccatgtac ggagccatgg gtatcgcagt
     5881 gggaatgctg ctttcggagg tgttggtact gctatttgtc tttgtgatct accgtggcag
     5941 catggcaggc agaagagaag cggaaagcgg tatgaaggga acggactctt tctccttgca
     6001 gctgcgtaca ttactgattc ctatgggtgg ggatattctc agagacatgt ttttattact
     6061 gcctttgtgg atcggcctgc ttttcttcca gaaaagatcg gcggatattt atgcatccgt
     6121 ttctgcgtat ggtattttcg tcggcagata tctgatcacg atcctgatgc ccgtagcaat
     6181 tttaagtgcg ggaattctgc cgggggtggc cagaaccggc agccatatcc gcaaaaaaga
     6241 agaaagatat gccagaactg catttcaggc aggagtacag gggacggtgg tacacggatt
     6301 ctttttcacg gcatggatcg cggtactggc aacaccactg tcacaggctt tggataaatc
     6361 tgcggaaatg gcacttaccg gtttgttcac tacgggatct tcgctgattc tgtggcttct
     6421 gcttttgttt tatttttcgg agatactgga acttcggggg gaaaagcatc tgctcctggc
     6481 aggatacggc attctggata tcgtgagcat cgtgatattg ctgattcttc tgaatacggg
     6541 aaatacaaca acggcattat ctttaagcct tgtgctgggg acggcagcgg gaggaattgt
     6601 tctcggcata cttgcctgcc ttcgaatgaa gacctggccg gatctgttgc gaacactggc
     6661 aattcccacc ggaagctgct gtgcctgcgg attgctggct tttgggctgg agaagatctg
     6721 cctgccccat ctgggggcaa tggtaacggt gctgctggaa ctggtgatca ccggtgcggt
     6781 ctactggttt ttgcttttga tgttacgcag tttccgtgga caggatctga attatgttcc
     6841 cggcggtaaa gtgctgcgtg ctttcggaaa aacgctccgg ctgctctaaa actgttttac
     6901 ggatgtatat tttcgataaa ataactgata ctgatacaaa gagtcaatag aaacatcatg
     6961 gatgtgaggc agtatgaaat ttgtaaaacg tatcagttac ttttttgtga ttccggttgt
     7021 gtttttaatt cttggaattt ttcttggtgt ctggggcatt cattttttct atcccggaca
     7081 atggcaggat ctgaatgaga accgacttgc cctgccgctc acggaagagg agactgcggc
     7141 agacacagat ccttatgagg cggcacccag tgtggaggcg gcagcctccg gggagacact
     7201 ttgtgcggat accgaatatg tgttggaaga ggcagatatt gtacggggta ccacagtgga
     7261 gacctcatgg aagatcccac ataaatatat tgggatgaac agggaaaatt ttctggagac
     7321 catggaactc tatgcggccc atccgccatt gtcggagatc gagcggggct ttgtggggtt
     7381 ggaggtattg tctttttccc gggaaaaagt aatggtccgt atggattacc gttatgtgca
     7441 gcccagcgca agtttttatc tggcggtgtc ggacaatgaa gtggtggtct atctggaaga
     7501 cagatccacg gtatatatca ataccgggat ctcgctagaa gaattgccgg aacagataca
     7561 gctccagatc atggacatgc tgtttgttcc ggatgaagaa acattgtatg attttctgga
     7621 gacgtattcg tcctagaatt ctgaataaag gtgatgcaaa tgaaaaaaca gatcggtgtg
     7681 atcggcggcg gtgctgccgg aatgatggca gccataaccg cggcaaaaga aggtgcacag
     7741 gtgacgatcc tggagcgtaa tgaccgtgtg ggaaagaaaa tattgcagac aggaaacggg
     7801 aaatgcaatc tgggaaatcg tgcgctttcc gtggagtgtt atcacggtgg ggatttggaa
     7861 tggatccggg atgtgttgga acattttgat acggaggata cgatacgctt tttccaaagt
     7921 atcggtctga tggtaaagga gaaaaatggc tatctctatc ccgtgtgtga gcaggcggct
     7981 gcggtgctgg acgtgctgcg ttatgaatta caggcactcg gtgtggagat acagtatcag
     8041 tgcaagatca caaaggtcga aaggctggcc tccggaaagc tgcaggtcag tgacggagaa
     8101 aagacatggc agttcgatgc ggtgattgtg acctgtggtg gaaaagccgc accggctacc
     8161 ggatcggacg gcagtggatt taaaattgca aaaaaaatgg gacacacctg cattccttca
     8221 gtgcccgcac tggtgcagct gcgctgtcag gagaccgacc tgctaaaggg gattgccggt
     8281 gtccgggcgg acagtgagat ccgggtcttc tgtgacggcg gggaaatatg ccgggaacgg
     8341 ggagaactac agctgacgga ttacggcatc tcaggcattc ccattttcca gctaagccgc
     8401 agggtgaact atctgctcag ggacaggcgg aacgtgacag cacagattga ctttttgccg
     8461 gatctgacac aggaaagcta tgaagaatta caggcagggc ggaaactttt acagggagac
     8521 cggacagcag aggaattctt taccggaata ttacataaaa aactgaccgc tgcgctgcta
     8581 aaaatggcag ggattcgtct ggctactccc atgaaggaga tcccggagga aaagattcat
     8641 gcttattata atctctgccg taacctgaaa ctgcatatca cagagagcaa tccttacgaa
     8701 aatgcacagg tatccgccgg aggcgtggat gtacgggaag taaagcctac tatggaatca
     8761 aagatctgct ccggggtcta ttttgcgggg gagatcctgg acgtggatgg ccgatgcggg
     8821 ggctataatc tccaatgggc ctggagcagt ggttatctgg cgggatacca cgctgcgtta
     8881 gcataaaata attttttaca cgtgacgtat gcctgcgtaa ggtgcaaaag ctccggaaag
     8941 gcggcaggtc attgaaagat atgaggatga aaatgatcag attaaatcag ctgaagcttc
     9001 cggtagatca tacccgggag cagcttatac ataagacggc acagtactta cggatcccgg
     9061 cggcggatat cctcgaactg cagatcgtaa ggcagtctct ggatgccaga aaaaagccgg
     9121 cactttttta cagttacagt gtgaatgtaa cggtgaaaaa ggaagaaaaa gtatataagg
     9181 atgcctgccg cagacttgga aaagccaatg ttctgctgac cgaaaaaacg gaatatcttt
     9241 ttcctgcaga aggttcaacg cagcagaagc atcccactgt gatcatcgga atgggtcctg
     9301 ctggactttt ttgcggatat tatctggcac agcacggcta tgcacctgtc attctggaac
     9361 ggggtgcaga cgtggatgcc agacaggcgg atgtagaagc tttctggcag acgggcagat
     9421 taaagccgga ttccaatgtg cagtttggag aaggcggtgc cggaactttt tcggatggta
     9481 aactgaatac tttggtaaag gacaaatacg gcagaaatat ggaagtattg aaaactttcg
     9541 taaagcacgg tgctgatccg gcaattcttt atcaggccaa gccccatatt ggtacggatg
     9601 tcctgagcaa agtggtaaaa agcatgcggg aagaaatatt gcgtatgggt ggggaagtcc
     9661 gatttcatag tcagatgacg gagatcctta cagagaacgg gcaggtcaca ggagtaaagg
     9721 tgaatgacac ggaagtgctc ccctgtgagc aggtggtact ggcaccgggg cacagtgccc
     9781 gagatacctt ttccatgttg taccggaagc agtttcccat gtcggcgaaa ccttttgcag
     9841 tgggattccg tgtggagcat tcccagagcc tgatcaatat gagtcagtat ggcgctgagt
     9901 cggtaaaagg tctgggtgca gcggcttata aggtgacggc taagacaagc accggcagag
     9961 gagtgtattc tttctgtatg tgtccgggcg gctatgtggt caacgcatct tccgagccgg
    10021 gaagactggc ggtcaatggt atgagctaca gcggccgtga cgggaaaaat gccaacagcg
    10081 ccattatcgt tgcggtgact cctgcggatt acggttccga tcatccactg gcaggaatcg
    10141 aattccagcg gggactggaa cagagagctt atgaattatg cgatgggaaa ctgccggtac
    10201 agcggtacgg agatttcagg gagaaagtca ccggagagca tcctgcggaa acggacggag
    10261 tacaaagaag tggcccggaa gcattggaac cacagtgtaa aggtgcttat gaatgggcgg
    10321 atcttaccgg aattcttccg gcagagtgta accgggcctt cgtggagggg atggacagct
    10381 ttgacagaca gatccacggc tttgcctctc cgggaacgat cctgaccggt gtggaaagca
    10441 ggacgtcttc cccggtgcgg atcgaacggg atgaagagtt acagtctgcc atccggggtt
    10501 gctatccctg tggagagggg gccggatatg ccggaggaat cacttccgcc gccatggacg
    10561 gtatccgcgt agcagaacag atcgcttcac agtttgctcc gctacagcgt tgacgtatgg
    10621 cagaaaatgt tatatgattc atatattcag agattgcatc ctgcagatcg gtttttcggg
    10681 ggaggcgctg aatattactg taataatatg gacttagaaa ggcaatcacg tgaataacat
    10741 aagggaacaa atcaaggata agaaaagaat cgtcgttaag atcggttctt cctccttaca
    10801 gcatgccgag acgggagacc tggattatat ccgtctggaa aagctggtaa gagagctttg
    10861 cgatatcagc aatcagggga aagaagtcgt acttgtgact tccggtgcta tcgccgctgg
    10921 aaaacaggca gtgcatttaa agactacgga ggggcagact caggcagaat ccatggcgat
    10981 caagcaggcc tgttccgcca tcggccaggc aagactgatg atgacctacc agaagctttt
    11041 tgcggagtac aatcaggtgg cagcacagat cctgatgacg aaaaatacca tcgtggacaa
    11101 tctgaaccgc tacaatgccc ataatacctt cacccagcta ttgaaaatgg gagcaattcc
    11161 cgttgtcaat gaaaatgata cggtagcgac ctacgagatt gagattggtg acaacgatac
    11221 cttgtcggct attgtggcag cactgatcga tgcagatctg ctgatcctgt tgtcagatat
    11281 cgatggcctc tacaccgatg atcccaggca gaatcccaat gcggaattta tcgaacaggt
    11341 ggatgaactc acggaggaat tcatgcatat gggaaaggcc agtaccggca gcaatgtggg
    11401 gaccggcggc atgaatacca aaatgatcgc ggcgaagatt gccaccagtt cggatgtgga
    11461 tatgatcatt gccaacagca aggacatcgg agtacttcat cgtctgttat ccggtgaaaa
    11521 cgaaggtact ttattcgtgg cacataagga tgaaagcttt gatctgccgg aatttgtaca
    11581 gaatctgcac aaaaatcagt gatgatacgt caacgaaaag aggaatacat tatgaatatg
    11641 gatgaaagaa taaagcgcat caatgagctt tatcataaat cccagagcga gggactgacc
    11701 gacgaagaaa agctggaaca gtccattctc agacaggaat acgtggacag catcaagcgc
    11761 aatatgaagg cgcagctgga cagcatcgaa atcgaaaggc cggacggcac cattgaaaaa
    11821 ttaaagccgt cctataaaaa gaagaaatga gcactgaaga acgcagagaa gtgaaaaaga
    11881 aactgcgcag ggaagtgctg gcccgcaggg cggcactgac ggagccggaa cgggagagag
    11941 ggaatcttct cattaccgag cggattctgg gacaccagtg gtattacctc tcggatacga
    12001 ttctgggctt tgtcagctac ggaagtgaaa tctgcacgac agagattctg gagaatgccc
    12061 tgcaggatgg caaacgtgtc tatgttccga aggtggaagg tcaggaaatg cgtttttacc
    12121 ggattcaatc cctggcggaa ttgaaagagg gctataaggg aatcctggag ccggaaggca
    12181 atacggagag ctatgaatat gctcccgaag aggcagcaaa ctgtctgctt ctgatgccgg
    12241 gaagtgtatt tgatcccctg ggcaaccgga tgggatacgg cggcggattt tatgaccgct
    12301 atctggcaga cagggaagcc ttaagacttc gcagtattgc tgtcgggttc cgctgccagc
    12361 aggtcgaaag tgttcctgtg gaagagacag atgtaaagcc ctatcaggtg attttggtat
    12421 aaatctttgg ttttggaaag gcggggaagg aatgtcggaa cttttagaac tgggaaaaaa
    12481 tgcctgcgat gccaaatatg tattgcagaa gcttactaca gaagagaaaa acaaggcgct
    12541 gcagacagcg gcaaatgatc tgtgtgaaag acaggaagag atccttgcag ccaacgtact
    12601 ggatctggaa aacgggaaaa acaacggaat gaatccggga ctgctggatc gtctgaagct
    12661 taccggagag agaatcgcgc agattgcaga gggccttcgg cagattgcgg ctcttccgga
    12721 ttgcatcggt gagaccatgg aacatttcga acgtcccaac ggactgaaaa tcgataaagt
    12781 cagagtaccg ttaggggtta tcggcattat ctatgaggct cgtcccaacg tgacggcgga
    12841 tgccttcgga ttgtgcttta agacgggaaa tgcagtgatc ttaaagggag gcagcgatgc
    12901 cattcattcc aatatcgcta ttgtcaaagt attacaggat gcactggaag cctgcagtat
    12961 gcccaggact gccgtacagc tgatcgaaga tacggatcgt gcggtcactg cgcagtttat
    13021 gaagatgaag gaatatgtgg atgtgctgat cccaagaggc ggtgcgggac tgatccgcag
    13081 cgtggtggag aacagcacga tccccgtcat agagaccgga accggcaact gccacgtcta
    13141 tgtggacaag gatgcggatg tggatatggc ggtaaaaata ctgatgaatg ccaagaccca
    13201 gcggatcggt gtctgcaatg cctgtgagtc tctggtggtg catcgtgccg tccgggataa
    13261 gttccttccg aagatggcgg aagccctgaa gacgaagaac gtagaactgc ggggagacga
    13321 aagcacccgg gagatcctgc cggactgcat tcccgctaca gaagaagatt acggtacgga
    13381 gtatctggat tatattctgt ctgtcaagac tgtggacagc gtggaagagg ccattgccca
    13441 tatcaaccgt tacaatacga agcattccga atgtatcgtg acggaaaata cggagactgc
    13501 agagaaattt ttaaacgaag tggatgcagc ctgtgtttat gtcaatgcct ccacaagatt
    13561 tacagacggt ttcgagtttg gatttggcgc agaaatcggt atcagcaccc agaagctgca
    13621 tgccagaggc cccatgggcc tgaaggagct gacctcctat aaatacaaga tacatggaaa
    13681 cggtcagatc cgcagataaa catttgcgac ccgctacagg tggataatct gcgtattctc
    13741 cggctggata ttttgttctg tatgcaaaga catctgtcgg aaataactat agaggaagag
    13801 gagaagattt atgaaagaag taaatggaag tatttggcac actatgagca cccagagagt
    13861 cactcccaca gattttgtct gtgtcatcga gatctccaag ggcagcaaga acaagtatga
    13921 actggacaag gagacaggcc ttctgatcct ggatcgtatc ctgcatacct ccactcatta
    13981 tcctgcgaac tacggtttta ttcccagaac ctacggtgat gatggtgacc cgttagacgt
    14041 attgttactg tgttccgagc cggtacagcc catgacactg gtaagagcgt atcccatcgg
    14101 cgtgatctcc atgctggaca acggcaaaaa tgacgagaag atcattgcag ttcctttcaa
    14161 tgatcccaat tacaactgct atcatgatat ttccgagctg cccagtcatg tggtggacga
    14221 gatggagcat ttcttcacag tatataaggc actggagaac aagaccactg ctgtcaatga
    14281 aaagggcaac agagatgagg caattgaagt cattaaaaag tgtctggaca attacattga
    14341 tacctttatc aagagttggt aagctgttga taattgattt tcaacaatca caaagcaccg
    14401 tatttcccat gcagttcatt gggggatacg gtgtttattt ctataaacta ttgaaaaaaa
    14461 ttgtcaaaac ccctagattt tttaaaggaa atatgagaga atgaacacga ccattatgat
    14521 attattttat caatagtatt ataagtacat attgaaagga gatttcacat gatttattca
    14581 aaagaagttg aagaaatgtg tcccgttgca cagggcgtac atcatggttg tgctcccatt
    14641 cccgaagagg caaaatgggt gcaggcaaaa gaagttaagg atatttccgg tctgactcac
    14701 ggtgtgggct ggtgtgcacc tcagcagggt gcatgtaaac tgaccctgaa cgttaaggaa
    14761 ggtatcatcc aggaggctct ggttgagacc gttggatgtt ccggtatgac tcactccgca
    14821 gctatggctg ctgaaatcct gcccggtctg accgttatgg aagctttaaa tactgacctt
    14881 gtatgtgatg caatcaatac cgctatgaga gagctgttct tacagatcgt ttatggtcgt
    14941 acccagagtg cattctctga ggaaggtctg cctgtagggg caggtctgga agaccttggt
    15001 aagggcttaa gaagccaggt tggtactatg tacggtactc tgaagaaggg tccccgttat
    15061 ctggagatgg cagaaggtta tgtaaccggt atcgctctgg acaaggatga tcagatcatc
    15121 ggttaccagt ttgttaacct gggcaagatg accgacttca tcaagaaggg tgacgatgcc
    15181 aacactgcat gggagaaggc taagggccag tatggtcgtg tagacgatgc agttaagatc
    15241 atcgatccca gaaaacaata agatacagga ggacagaaaa aatggcttta tttgaatcat
    15301 atgaaagacg tattgataag atcaacgctg tactgaacag ctatggtatc tcctctatcg
    15361 aagaagcaga gaagatcaca aaagatgcag gcctggatgt atacgatcag gtaaagaaga
    15421 tccagcccat ctgttttgaa aatgcttgct gggcttacac tgtaggcgct gcaatcgcaa
    15481 tcaagaaagg ctgcagaaag gcatctgaag cagctgcagc tatcggcgaa ggtttacagg
    15541 cattctgtat ccccggttcc gttgctgata cccgtaaggt aggtcttggc catggtaacc
    15601 tgggcaagat gctgctggaa gaggatactg actgtttctg tttcctggca ggacatgagt
    15661 ctttcgcagc agcagaaggt gctatcggta tcgcagagaa ggctaacaaa gttcgtcaga
    15721 aacctttacg tgttatcctg aacggtcttg gtaaggatgc tgcaaagatc atttcccgta
    15781 tcaacggatt tacctttgtt gagacctctt atgatcctta caccaacacc gttaccgaag
    15841 tagagagaat cgcttactcc gagggacttc gtgctaaggt taactgctac ggcgctaaca
    15901 gcgttcccga aggcgtagct atcatgtgga aggagaacgt agacgtatcc atcaccggta
    15961 actctaccaa ccctaccaga ttccagcatc ccgtagctgg tacttacaag aaggagagac
    16021 tggaagcagg caagaagtac ttctccgtag cttccggcgg cggtaccgga cgtactctgc
    16081 atcccgataa catggctgca ggtcccgctt cttacggttt caccgatact ctgggccgta
    16141 tgcactctga cgcgcagttc gctggttctt cctccgtacc tgcacacgtt gagatgatgg
    16201 gtctgatcgg catgggcaac aacccgatgg taggtgctac cgtagctgtt gctgtatcca
    16261 tcgaggaagc tgctaaggct ggtaagttct aaaacataaa atcagataaa tcaaggattt
    16321 caaggctccc gacattgttc gggagtcttt ttttccacaa aattactttg taatcgaaca
    16381 tgcattctgc tataatcatt tatagaaagt gtttgaaagg aggcagcaat ggaatcacaa
    16441 tcccatactt atattgccat ggatctgaag tctttttatg cctccgtgga gtgcgttgac
    16501 agagggctgg atcctttgaa tacgaatctg gtggtggcgg atgcttcccg gacgcagaag
    16561 accatctgcc tggcggtatc tccctctctg aaatcctatg gaatcccggg acgggcaaga
    16621 ctgttcgaag tgatctccaa agtaagagaa gtaaatgcca tgcggaaagt gcggtcccct
    16681 ggcggcagat ttttgggaaa aagcaccttt gcggatgaat tgcagaggca tccggaactg
    16741 gaagttggtt ttgtgaccgc cacgccccgg atggcgagat atctggaata tagtacccag
    16801 atctataata tttatttgaa atatatcgca ccggaagata ttcatgtcta ttctattgat
    16861 gaagtgtttc tggatgtgac acaatacctg aagacctatg gtatgacagc gagagaacta
    16921 gcatccagaa ttgcaacaga gattctggag aaaaaagggc tgaccgccac agcaggaatt
    16981 ggaaccaatc tgtacctgtg caagattgcc atggatattg tagcgaagca tatggaaccg
    17041 gatgataacg gagtccggat tgccgaattg gatgagatca gctatcggga acttttgtgg
    17101 gatcacaggc cgctgacgga tttctggaga gtcggcgccg gataccgcaa gaagctggaa
    17161 gccgccggaa tgtttaccat gggcgatgtg gccagatgct ctatcggagg actgggagat
    17221 tactataacg aagatttgct gtaccggatg ttcggcatca atgcggagct tttgatcgat
    17281 catgcctggg ggtatgagcc ggtgaccatg gacctgatca aatcctatcg accggagact
    17341 aactgtatca gctccggcca ggtactgcat tgcgccacgg attttatgca ggccaagatc
    17401 gtagtccggg aaatggcaga acagctggca atggatctgg tggaaaaaaa gctggtcaca
    17461 gatcagatcg tgctgactgt tggctatgat attgacaacc tgaagattcc ggagatcgca
    17521 gatcattatc atggtgaaat cacggtagat cgttatggca gaagcgtgcc gaagcatgcc
    17581 cacggcactg gaaaccttga tgcaatgact gcatcagtca gcaggattac agagacaaca
    17641 atgggattat atgaacggat tgtagatccc atgctgctga cccgaagaat cactctggtg
    17701 gccaatcatc taaaggatgc ggacagcgta aaggaagagc cgttctacga acagctggat
    17761 ctgttctccg gaggcttttt gcaaacgggg cagaaggaaa cttcccggga aacaaagcag
    17821 ctggaaaagg aacggctgga aaaagaaaaa cacttgcagg aagcaatgct cagcgtaaaa
    17881 tcaaagtatg gaaagaatgc gattctgaag gctgccgatc tgcaggaggg agccacaacc
    17941 atagacagga accgtcagat tggcggacat aaggcataat atttatcgga ccacaaaatc
    18001 cgataacagg aaaagagtaa aaagatgaat gataaatata aggacatgct tcatctgcct
    18061 cacccggtgt cagccaccca tccccgaatg gctttacagg accgtgctgc acagttttca
    18121 ccgtttgcag cattggcggg atatgatgac gctctgagag aaacggcccg ccggacagac
    18181 cgttttgtgg aactggatga ggacaggaag caggagctcg accgccagat ttcttatttg
    18241 caacagcatc tgctggacac agtgccggtg aaaattattt attttgtgcc ggatgagaaa
    18301 aaggacggcg gcagttatac tgccgtagaa ggctgtgtcc gcaaaatcga tgagaacaca
    18361 aaaagccttc ggatacaggg aacagaaatt ccggtagaga ggatctacga gattgatttt
    18421 ctgtagttac attgtaaata gttcagagaa atgctaaaat attaccaata gtattttgag
    18481 aacaagaatg gaaaggaatt tctgacaatg aagaaaaaat gtatcagtac agcaacactg
    18541 tcagtgcttt tgcttgtact gtttcttcct gttttctatt atatagtatt ctacgggacc
    18601 aatgtgaact acaacgaaat gcataagatc attaccgtgg agggaaataa agtactatcc
    18661 ctgtgcgcgg tgatcggagt ggctgtactg ggggcagcgt actattttct gcgaaagatt
    18721 ccttacaccg gacgcatagc agtctggttt accgggatca cactggcagt ctgtattctg
    18781 ttctgtcttg tgaatataaa aatatccaaa tgcattgcat tttacggtgg ctgggattgc
    18841 ggcatggtgg ccaattccgc ccgctggctt tatgagggcc agacactggg gtatgacgat
    18901 tactatacga tctacagtaa taatattccc gttacatggc ttttgtatca gttgtattct
    18961 tttgcctcag gacttaaggg gtatccctac aatccggaat ttatctggat ccagttccag
    19021 tgcgtcatgc tgtcactggc ggtattctgt tcagtacttc tggtccttca ggtcagcagg
    19081 aaccttggaa tttccgtgat tgccctggtc tttcagagca tctttctggg aatctctccc
    19141 tggaagatca ttccctatac ggacggaagc accattgcca tgccgatcct gattcttcta
    19201 ctgtattcat tgttccggaa gaatgggaga aaacggaaat accttttatg gtttttgata
    19261 atgtttctgg ggtgccttgg cggaatcatg aaagccacct gctatgtcac attgattgcg
    19321 gtagtactgg tggatctggt atggtctgcc tgggaagagg tgccaatgcg taagcggctg
    19381 cagaagaccg gcttaaaaat cttgcttctg atagcggctt tcggacttgg ggctttctgt
    19441 aagcagggaa tctactgttc cgtacagtat gattataatg cggatatgga gatcggctgg
    19501 agtaattata tgtataacgg cctcaatgag gatactaccg gggcctgttc cggagaggga
    19561 cttgagatcg tacgatcttt tgctggtacg gataaaaata cccggataca atatgagatg
    19621 gaaggaatcc gcaaaagaat cggtgatcgt ggtatttggg gtacaatccg gttctggctg
    19681 cgtaagcagg tcatgaattt taacgatggg acgttcagct ggtatcagga agggtatttt
    19741 caggcgtggg aatatccgtt gaacattgac agctccggga aagaagctct gagggctttt
    19801 tactggcagg acggcagcaa ttatatctgg tttacgacga tcagccaggg attatggctt
    19861 tttgtactgt tgggagtcat aacagaggct ggtatgcttt tgtggacggc ggtgtcaaca
    19921 atccgcagac ctaaataccg gacagaagag aacctgtctg accggctgtg tctttctact
    19981 gtgatgatcg ttacatttat cggtatgttt ttatttgtta tgttatttga ggcaagagcc
    20041 agatatctat ataataccat tccggtattc tctgtcatgg cagtttttgg atattacgaa
    20101 ttgtatcgga aattattcat attttgtgac aaaaggcgga aataacttcg ttttccggta
    20161 gaaattgcac gttatgagag cctataatgt gaaaatagca ctggtaaaat atataaacat
    20221 cctgtataat gtacagtaat gactgagaaa tgagtcagga acaaaaaaga gggaggaaga
    20281 ataaatgtac ttttgtaaga attgcggcga gccttatatg acagacgaag ctgtgatgtg
    20341 cgtaaagtgc ggtgtggcca agggacaggg gaataattac tgccataact gcggtaagcc
    20401 tttagctccg gatgctgcag tatgtttaaa ctgtggcgta gccaataagc aggcaccggc
    20461 accggatgca aaatccaaac tggtagccgg tctgttggca attttccttg gtacgttcgg
    20521 cgtacataat ttctatctgg gctataccgg taaggcagtt acccaattgg tactttccat
    20581 cgtgggaatc cttttatgct gtgtggtagt cggcgtattc attgttatgg gggtaggtat
    20641 ctggggactg gtagaaggta tcatgatcct gaccggcaag atcgacacag acggcaaggg
    20701 taatcccctg gcagattaat atcaggcctt ttccaggcca gaaagaggag aaaatggacg
    20761 gacagaattt tgaaaatgaa aacaatgtta cagtagatac tactgcaaca gaagtaagct
    20821 ccgcagcaga aactgtacag gagaccccgg taacagaaca gcctgtgaac aattatcagg
    20881 attataccgc aaatgtacag cctcaggcag ctgcaacagc tcctgtacag cagccggaga
    20941 acaaagaaac caatgtactg gctattgtca gcctggtact gggtatcctt tccatcgtac
    21001 ttggatgctg caccggatgg atcggcgcac tgtttggaat cggtggtatc atttgcgcag
    21061 tatttgccaa caagcagggc aagaccggcc tgtcaaaggc tggactgatc tgttctatca
    21121 ttggtattgt actgggaatt atcattacaa tcttagctgc tgtattttct gtagcattta
    21181 tgagtgaact tgcaaactac tactaaaaac cagcggagcc tggaagatca gctttatatt
    21241 gcaggatgga tatttctgct ggtcggaagc atcggaagct ttctgtatct tcatataatc
    21301 gttccgagac tgcagggata tacctgcgtg gtctatcggt tatttgggat ttactgtccc
    21361 ggctgtggtg gaacgagagc ggtaagtgca ctgcttactg gacatccgct actggccacc
    21421 tggtatcatc cactggttac gtatactgtt gttatctttg gaggttttat gctaagtcag
    21481 acccttgcca gattacatat tggcaggatc aaaggctggc gctttcatga atggcatctg
    21541 tatgcggcgg tagggatcat gatagggaac tttattctga agaatatttt gcttctgaag
    21601 tttcacatta cattatgact gtatttgcat tttttacata tacagaatat tttatcgcac
    21661 aaaagtgcag agggagaaaa catgaataaa aaaactacgg gtatcattgc ttacattacc
    21721 tggatcggct ggatcgttgc tttttgcgca ggtgacaaag agggtgctaa gttccatctg
    21781 aatcaggctc tggttattct gatcggccag atcgttgcag cagtaatcaa ctacattcct
    21841 tatgtaggtg gtattgttgg cactatctta agcatctttc tgtttgtatg ctggatcatg
    21901 ggtctgatct atgcttgcca ggagcaggag aaggaagttc ccctgattgg tcagatcaag
    21961 attttaaaat aataaaaatg acaataagtc ataaaaagaa tccccggtgt tcattacatc
    22021 ggggattctt tttatgggtg cgcctggcaa cgcacgtttc tgtgattatt aaaaaattgt
    22081 aactattctg cagcagaagc agccgctgtt tcctctgcag tggtcagatt cacatcttcg
    22141 ggctgttcac cgtgcaggaa agtgatgatg atctggatcc gcttgtaagc tctggcataa
    22201 ttttcttttg catagtctgc ggtcagctga ttgtaaccac cgttatcata ggcatcgtgg
    22261 taactctcta ccgcagtggt catatattcg cttgcttcat cggcaagaga ttccagataa
    22321 tcaaattcag taggaaaatc caacgctgca aaatcctgaa aagcgctgtc cagctgatcc
    22381 agataaccga gaagttcatc ggtggcattt tcggatgtcg cgtctacgtt gttgatctcg
    22441 gtatctatat cggagatctt ggtacaaaaa gaatctattt cctccttaaa ctgtgtcagc
    22501 gcaggatcct ttccgcaggc ggtaagcgta agtgcggtca gcagtccggc cgtaagggca
    22561 agtatttttt tcttcaaaat tatatcctcc aaatcatttg atccatctaa aaatttatca
    22621 tattctgcag ttttacgcaa ccttataatg ttaaaacgtc ccctttacga ataaaaggac
    22681 attgtggtat gattaaaaga atcaagatac aggaaaagag agctatgaca agagaagaat
    22741 ttattacgtt aagtaaagac catattttat atctggacgg cgctacagga tccaatctgg
    22801 tgaaggcagg aatgccttcc ggcgtctgcc cggagcagtg gatcctggag catcaggatg
    22861 tcatgctgca attacagaag gattacgtgc aggcgggaac caatatcctg tatgccccta
    22921 cctttaccgc caatcgcgtc aaactggcag aatatcatct ggaaaaaaat atgacttcca
    22981 tgattcgtga tctggtggct atttcgaaga aagcggcaga gagtactccg ggacatccgg
    23041 tttatgtggc cggtgatctc acaatgactg gtgaacagtt aaagcccatg ggaaaaatga
    23101 atctggaaac actgatcgat atttataaag agcagattct ttgtctggtg gatgccgggg
    23161 cggatcttct ggtggtggag accatgatga gcctggcgga gaccagagcg gcactgatcg
    23221 cggcgaaaga agtctgtgat ttgccggtaa tcgctacact gacctttgaa gcggatggaa
    23281 gaactctgtt cggtacggat gcgaaaacgg cggccattgt gttggaaagc ctgggggcca
    23341 gtgctatcgg tgccaactgt tccacaggtc cggctcagat ggagaacatt atttcggaga
    23401 tggtatccca taccagaatt cctgtaattg caaaacccaa tgccggactt ccttttctgg
    23461 atgaaaacgg aactacctgc tacaacatgg aagcagaaga gtttgcagaa gagatggaag
    23521 tactggtcaa tgccggagct acgattcttg gcggctgttg tggtaccact ccggaattca
    23581 taagacagat tcatgaacga tttggtacag atgcgaaggt tgcagcatcc cgaagaccgg
    23641 atggaattcg gtatctgacc tccgaacgta ttacccacag ctttggtctg gatgacggct
    23701 ttttcgttgt gggagaacgt atcaatccta ccggcaagaa agcacttcag gcacagcttc
    23761 gtgagggcag ctttgaaaag gtgatacagt ttgccgaaga acaggaagcc tgcggtgcca
    23821 aggtactgga tatcaatatg ggcatgagcg gtatcgacga gaaagccagc atgcttcggg
    23881 ctctggagga agtcagcggt gtcacgaacc tgcctctttc cctggattca agctacgtgg
    23941 aagtcttaga agctgcactg cgtaattatc ccggacgtgc tctggtcaat tctgtatctc
    24001 tggagacgga aaaatttgaa aaactacttc ccattgtagc caaatacggt gctatgttta
    24061 tcctgcttcc tttgtcggat gcgggactgc ccaaggatat agaagagaag aaagagatta
    24121 ttcacaagat ttatgaccgg gcactttctc tgggaatgtg caaagaagat atcgtggtgg
    24181 acggtctggt ggcaacggta ggtgccaatc cgaaagctgc actggaaacg ttggagacca
    24241 tccgctattg caaagagaac ggttttgcca cgatctgcgg tctgtccaac atttccttcg
    24301 ccatgccgga gagaagtttt gttaataccg cattcctgac gctggcaatt caggcgggac
    24361 tgaccatggc tattgccaat ccttcccagg aacttctgat gagctgtgct ttggctgcgg
    24421 atctgctgtt gaacaaagaa gaagccgctc ttcgctatat cgagtacgcc ggtggcgtca
    24481 gggagcgcag agaagagaaa gaagcggaac tggcaaaaaa actggcactg ctggaaaatc
    24541 agggaacaac cgccaagaca ggcactgccg gcaatgccgc caaagaaacg actactgata
    24601 acggtccgca gatcaatgaa atgcaggaca agctgaagac ggcggtactg aaaggcaacc
    24661 gtaacggcat tgtaaagatc acgaaggaag cattggaaag cggagagaaa cctgcggagc
    24721 tattgaatca ggtactgctt cctgccatta atcaggtagg tgaattcttt gaccagggca
    24781 agtattttct gccgcagctg attgccagtg cggaagccat gaagaattcc atcgaggtgt
    24841 tggaaccgtt gctgcagacc ggaggcaccg gagaagaaat gccggtggtg gtcattgcca
    24901 cggtggaagg agatatccat gatatcggca agaatctggt ggcactgatg ttaaaaaacc
    24961 acggattcca tgtgatcgat cttggcaagg atgtgccgca ggcgaaaatt ctggagtcgg
    25021 cgaaagaaca tcatgcggaa tttattgccc tgtcagcact gatgaccacc accatgcaga
    25081 gaatgcggga aatcgtggca gcagcaaaag aagaaggcat caccgccaag atcatcatcg
    25141 gcggcgctgt cattacgcag gaatatgcgg atgagatcgg tgccgacggc tattccaagg
    25201 atgcggcaga tgccgtgaaa ctggccaaaa gcctgatgta aaagagtctg aaacaacaga
    25261 aataatggag tatagataaa ggagcaaagc aaaatgattg gagcagatgc gattattaaa
    25321 tgtctggaag cagagggggt aagtactgtt ttcggatatc ccggtgttgc catatgtcct
    25381 tttttcaaca gcattctgga ttctgacatc aaaacggtgt tgatccgtac agaacagaac
    25441 gcggcccatg cggccagcgg tgttgcccgt atgaccggaa gacccggtgt atgtgcggtg
    25501 acctccggcc ccggtgccac caatgtgatc accggtattg ccacggcatt tgctgacagt
    25561 atccccatga tctgcattac cggtcaggtc aacagtgaac tgctgggcag tgatgtgttc
    25621 caggaggcgg atattaccgg cgccgtggaa tcttttgtaa aatacagcta tctggtgaaa
    25681 aatgtaaatg atattcccag agtgttcaag gaagcatttt atattgccaa taccggtaga
    25741 aaagggccgg tattgatcga tattcccatt gatatccaga atgcaacgat caaaaatttc
    25801 cattatccgg aagaagtgaa tctacgtacc tacaagccta ccgtcaaagg acatgccgtg
    25861 cagatcaaga aggttatcaa agagctggag cgggcgaaac gtcccattat ctgtgccgga
    25921 ggaggagtcc tgctcagtga ggccgaggag gagctgcgca gctttgcgga agaacacggt
    25981 attcccgtgg tgtccaccat gatgggtatc ggtgttatgc ctaccgaaca tccgctttat
    26041 tatggaatgg tgggcaataa cggtaagact tatgccaacc gtgccatgaa cgaatcggat
    26101 ctgctgatca tggtgggagc cagagtggca gatcgcgcgg tcagtcagcc agatctcatc
    26161 acaagaaaca aagtgctggt gcacattgac gtggatcctg cggagatcgg caaaaatgcg
    26221 ggtccttcca ttccactggt aggtgatgca aaacacatct tccaggattt tcagaaagaa
    26281 gaatttgact gtaactacga ggaatggctc actacattga atgaatatcg ttctaccatg
    26341 gagaagaaga gaacccccaa tcccgattat gtggatcctg cagcatttat cacaagactt
    26401 tccgagaaga tgcaggagga cggtgtctat gtggcggacg tgggacagaa tcagatctgg
    26461 tcctgtggct atcacattgt gaaaaaaggc aagttcctga cctccggcgg tatgggaacc
    26521 atgggttatt ctattccggc agccatggga gccaagacag cggccatgga taagcaggtt
    26581 attgcggtat gcggtgacgg ttcctttcag atgtccatga tggagctggc tacgatcaga
    26641 cagcacaata tcccggtaaa gatcatcgtg ctaaagaaca attatctggg tatggtaagg
    26701 gaataccagc actataccta caaagatcat tattctgtcg tggatctgtc cggaagcccg
    26761 gatctggaga aattatctgc ggcctatgat attccgtatc tgcgattgaa caacatggaa
    26821 cacgtggatg agattctgga cgcttttctg gcggaagaca ataccatgct gttagaatgt
    26881 ctgatcgatc cgatggatct ggtaaaataa cggaagggag ctgtacttaa aatgaagaga
    26941 atatattccg tattggtaga gaaccgttcc ggtgttctgt gcaaggtagc cggtctgttc
    27001 tccagaagat gttttaatat tgacagcctt gccgtgggag agaccgatga ccatgcagtt
    27061 tcctgtatga ccattgtaag ctccggtgat gagcgtaccc tagagcagat tgaaaagcag
    27121 ctgaataaga aactggatgt catcaaagtg aagactttgt cccaggaaca gtgctatacc
    27181 cgggaattaa tgttattaaa agttaaatat aacaagaata atcgtaggga tattatagaa
    27241 atctgtgaca ttatgaaaac agagatcgta gatatgtcaa aacactttat gatgctgcag
    27301 atttgcgatg ctccggagaa aatcgacact ctgatcaaga tgcttcagac catcagtatt
    27361 gtggaagtag ctcgaacagg aaccttggca ttatcaaaat gcacggaaaa tgatgattaa
    27421 aatacatcaa tcataatgtt aaattcacta attgacattc tgtagtcatc ttgctatatt
    27481 aagaatacag ttgctgctcc gtcagggagg acggctgtag aaatacgaaa ggtatggtag
    27541 atattcatgc agaaaaaagt atttcagctg ttggttgaca acacttcagg tgtgttgagc
    27601 cgtattgccg gattgttttc ccgtcgcggt tacaacatcg aaagtattac ggcaggtgtg
    27661 accgccgatc cccgttttac cagaatcacc attgtgacat cgggagatga cgatattctg
    27721 gaacagatcg agaagcaggt gggaaaactt gtggatgtcc gggatattaa ggaactggac
    27781 ccggatgaca gcgtgtaccg cgaactggta ctggtcaagg tgaaagtaga tcccgaaaag
    27841 cgtctggaga ttcgccaggg ggtagaattc cttgccaaca atttccgtgc gaagatcatt
    27901 gatgtaggtg cggagaatct gatcgttgaa tttaccggta atcacagcaa ggtcaatgcg
    27961 ctgatccagc gttttacgga agagtacgaa gtgttggagc ttgccagaac cggtatcaca
    28021 ggtctgggaa gaggtatcga gaacgttacc tatattgaag attgaggact gacaggcgta
    28081 agacctcaca ctttatgtta gctctaggga agatccctat cagttataaa ttttatgtct
    28141 cataacggag gagaagaaaa tggctaagat tttttatcag gaagattgta atctctcttt
    28201 attagagggt aagactatcg caattatcgg ctacggcagc cagggtcatg cacatgcact
    28261 gaacctgaag gattccggat gtcatgttat catcggtctg tacgagggct ccaagtcctg
    28321 ggcaaaggct gaggcacagg ggttcgaagt attcaccgct gcagaagctg caaagcgtgc
    28381 tgacatcatc atgatcctga tcaatgatga gaagcaggct gatctgtaca agaacgacat
    28441 cgagcccaac ctggaagagg gcaatatgct gatgttcgct catggtttca acattcattt
    28501 cggatgtatc gtacctccca agaacgttga tgttaccatg atcgctccca agggacccgg
    28561 acataccgta agaagtgagt atcaggctgg taagggtgtt ccctgtctga tcgctgtaga
    28621 gcaggatgca accggtaagg ctcaggatct ggcactggct tatgcactgg gtatcggcgg
    28681 cgcaagagca ggcgttctgg agaccacttt cagaaccgag accgagaccg atctgttcgg
    28741 tgagcaggca gttctgtgtg gcggcgtatg tgctctgatg caggctggtt tcgagactct
    28801 ggttgaggct ggttacgatc ccagaaatgc atacttcgag tgtatccatg agatgaagct
    28861 gatcgtagat ctgatctacc agagtggttt cgcaggcatg agatattcta tctccaatac
    28921 cgctgagtat ggtgactaca tcaccggccc caagctgatc accgaggaga ccaagaagac
    28981 catgaagaag atcctgtccg atattcagga tggtactttt gctaaggaat tcctgttaga
    29041 tatgtctccc gcaggcaagc aggttcactt caaggctatg agaaagcttg ctgctgagca
    29101 tccttccgag aaggtcggcg aagagatccg taagctttac agctggaaca acgagagtga
    29161 taagctgatc aacaactaat caactgtaaa aaatccaatg ctatgacaaa agggctgtgt
    29221 aagaatgtaa aagttctgac acagcctttt ttggcaaaaa atcagttgtt ttcaaaggaa
    29281 gaccgcaaaa acaaactgga aaatacatat taattgcgat aaaatgaata agtaagcccg
    29341 gaaaatagaa acgtggcatt gaaacatgaa acagatctgt ggacaggaaa agacataaat
    29401 gcgcagatca cggaaaggca tgaaaagcgg tatgaaagat tttgaagcaa aaacctctcc
    29461 caggcagaaa atggataccg aaagctggtt tatcgaatgt taccggaaca atcaggggca
    29521 tcctctgcgg acgttagtgc atctgtacaa gggaaattat cataaatttg ttctggcagt
    29581 catctgcttt tttgcaaagc atgcctgcgt ctgggtactg ccgatcatta cggccaatat
    29641 catcaatgat gtgacatccc acaacccgga tacttttcgg aacattattt tttacagtat
    29701 tctggaagca gggctgatcc tgctgaacat tccgatgaac tatctgtata cttcctacaa
    29761 gagtctggcc acccgctatg cggagacggg actgaggaaa gcattgatcc gaaagcttca
    29821 gcagttatcc atttcctatc atgtggagac tcagtccggg cgattgcagt ccaagatcat
    29881 gcgtgacgtg gaagcggtag agggactttc cacacagtta tttttaagca ttctgaacat
    29941 tgcgctgaat atcggtgtgg cgctggtggt caccatcagc aagagccgga tcgtatttat
    30001 ctttttcctt ttgaccacac cggtagcagc catcaccatg gtcactttcc ggaatatcat
    30061 gaaaaaacgg aacactgaat tccgtaagga aatggaagag acttccgcca gagtcatgga
    30121 aatggtggaa cttgtgcctg tgaccagagc acatgccctg gaggacgaag aagtggcaaa
    30181 aatgagcggc cagctttttg ctgtggcgga aaaaggctac aaactggatc tgatccaggc
    30241 attgtttggc tcggtgggct gggcagtatt ccagatcttc caggtgatct gtctggcatt
    30301 taccggatat ctggccattg gcggcaaaat tcaggcaggc gatatcacat tgtaccagag
    30361 ttattttgcc acggtagtca atcaggtgtc ctccatcgtc accctgctgc ctaccattgc
    30421 caagggtatt gaatccgtca attccatcgg tgaagtattg ttatctgaag atatcgaaga
    30481 taatgagggc aaggaaaaac tggaacaggt gaccggtact tttgattttg aggacgtatg
    30541 tttccattat aaaaataacg aacataatgt attgaaccat ctgaatcttc atgtgaagtc
    30601 gggagagact attgcactgg tgggagaatc cggcgccgga aagtctacga tcctgaatct
    30661 ggtcatcggt tttaatcagg cggaaagcgg aaaagtcctg atcgatggca aggatatcag
    30721 taagatcgat ctacgcacct accgaaagca cctggcggta gttccccaga cctcgatcct
    30781 gttctccgga acgatccgtg acaatatcac ttacggttgc gaaaatgtgt cggaggaaga
    30841 actgcagaaa gtgatcaaag ctgccaatct gtcggatctg atcgcatcac tgcctaaggg
    30901 tctggatacc atggtgggag agcacggcgg caaactgtct ggcggtcaga gacagcgtat
    30961 ctccattgcc agagcactga tccgggatcc gaaggtgatc gtactggatg aagccacctc
    31021 ggctctggac agtatttcag aaaagctgat ccagcaggcg ctgaacaatc tgacagaagg
    31081 gcgtaccacc tttatcgtgg cccacagact gtccaccatc cgggatgccg acaggatcgc
    31141 ggtcattgca gacggtcgct gcgcagagta cggcacttat gaagaactta tggctctgca
    31201 gggagaattt tatcagttga aacagattca gagctgatgg aaatcagaaa acagaaaagt
    31261 tgcttgaaac atatgttcgg ctatggtatg ataaacatat cgtagccgat ttttacaggt
    31321 tactatttgc aggacaggaa agacgaggac ttagtataca tgtttgcata tgtgaatggc
    31381 tttttggaag ataccacggt agataacgct gtgatcgatg tcaacggatt tggcatcaat
    31441 gtaaggattt ctgcggatac agcctccagg ctgccgggaa tcggagaaga agttcggctt
    31501 tacacttata cctatgtgaa ggaagatgcc tttttactat atggttttct ctcccgaagt
    31561 gatctggaaa tgttcaagct gtgcattaca gtcagtggaa tcggtccaaa gggagcgctg
    31621 gcaatattat cggtcatgga tgcagatgct ctgcggtttg ccattatgtc cggggatgcc
    31681 aaggcaatct ccagggcccc gggagtggga gcccggacgg cgcagcgcct gattctggaa
    31741 ttaaaagata agatctccat tgatgataca ctgatctcca gagagatcgc cgcaggcgga
    31801 gcccagggaa tcctgactgc cggcagcctg cacattgaca gtgagaagaa aaaggaagcc
    31861 gtggaagcac tggtggcact gggatacgga caggcagaga gcctcaaggc tgtcaatgcc
    31921 cttgaggatg tggagaccat ggattccggc gcgattctga aagctgccct gaaaaaattg
    31981 ttttaaggaa ccggataaat tatgcctaga agaatgatag agacaaatat cacagaagaa
    32041 gatatcaaac tggaaggctc tctgcggccc cagacactgg atgactatat cggccagtcc
    32101 aagatcaaag agaacctgaa ggtgtatatt accgcagcaa agcagcgcgg agatgcactg
    32161 gaccatgtgt tgttctatgg acctcccgga ctgggcaaga ccacgctggc aggaatcata
    32221 gccaacgaaa tgggtgtgca tatgaaggtg accagtggcc ctgcgattga aaagcccggt
    32281 gaaatggcgg cgatcctgaa caatctggag gagggagatc tgttattcgt ggatgaaatc
    32341 catcggctga accgtcaggt ggaagaggta ctgtacccgg ccatggagga ttactgcatc
    32401 gatattatga tcggaaaggg ttccagtgcc agatctgtgc gtctggagct gccgaaattc
    32461 actctggtgg gagcgaccac ccgtgccgga cttctgactg cacctctgcg ggatcgtttc
    32521 ggcatgatcc atcatctgga attttatacc atagaggaat tacagcagat catcatgcat
    32581 tccgcccgga tcctggacgt ggagatcgaa ccggccggag caaaggaaat ggccagaaga
    32641 agccgcggta caccgagact ggcgaaccgg atcttaaaac gtgtccgtga ctttgcacag
    32701 gttaaatacg acgggatcat taccgaggag gtagccagaa cggcattgga tctgatggat
    32761 gtcgataaga tgggactgga ccatatcgac cgcaatattc tggtcacgat cattgagaaa
    32821 ttcgacggag gtcccgtggg tctggatacc ctggcagctg ccatcggtga agatgccggt
    32881 acgatagagg atgtctacga accctatctg atcaagaacg gatttctgaa cagaacaccc
    32941 aaaggccgtg tagtcacaga tcttacctat catcatctgg gactgaattg atacgtgcag
    33001 cttacgaaat tttacttgcc accctataca ttctgatata taataaatct tgtattatat
    33061 ttttacgtaa atggcatata ttgtgttttt caaaagagtg ttaagagggg tgttgagatg
    33121 tcacctaaga cagatacaga agtgatcata ggcggaaaag tatttacttt aagcggctat
    33181 gaaagtgaag attatatgca gaaaattgct tcctacatca acggaaagat taccgaatac
    33241 gggaagctgg acggctttcg cagacaaccc ttggataagc agaacattct gctccagttg
    33301 aatattgcag atgattattt taaagccaag aagcagatct ccattctgga ggaagaactg
    33361 cagggcaagg aaaaagagct ttatgatctg aagcatgaac tgatttcagc acagatcaaa
    33421 ctggaaaatg cagaaaagca ggggaaggaa ctgcaaaagc agttaaatga agacgccaag
    33481 aagattgtac gtctggagac agaattaaaa gacaaataaa ttgatagctg tccgttttcg
    33541 ggcagctatt tgcttatatg aatcgaatgc aatagaggaa atctgaaatc tatgaaagca
    33601 gcaaaaaaag tagaattact ggcacctgcc ggaaatgcgg aagcctttta cggggctatc
    33661 catgccggtg cggatgctat atatctgggt ggcagccgtt tcggggccag agcttatgcg
    33721 gaaaattttt cggaagaaga actggttgcc tgcatccggt atgctcatct ttttcagcga
    33781 aaagtatatc tgacggtaaa cacactggta aaagaatcgg aattttccgg actgtatgaa
    33841 tatgtattgc cctattaccg ggccggactg gatggggtga ttgtccagga catgggagtg
    33901 tttgcatatc tcagagaaca ttttcccggg atggaactcc atggaagcac tcagatgacc
    33961 attacagggg agtatggggc agcttttctg aaggaacagg gggcctgtcg tgtagttccc
    34021 gccagagagc tgagccttgc ggagatccgc aggatcaagg aagtcacagg gcttgagatc
    34081 gaatgcttta tccatggagc catgtgttat tgctattccg gacagtgcct gttcagcagt
    34141 attctggggg gcagaagtgg caacagagga cgttgtgccc agccctgtcg cctgccctat
    34201 accactggaa aacatccgaa ggaatgttat cctttaagcc taaaagatat gtgtaccatt
    34261 gaatatattc cggagcttct ggaggcaggg atcgactcct ttaagatcga aggccgtatg
    34321 aagaagccgg aatatgccgc cggagttacg gcaatttatc gcaaatatat tgaccagtat
    34381 tatgcggatt ccaaagctcc tcagaagatt tctgcggcag atatgcatac gctttcctgc
    34441 ctctatatcc gcagtgagag acaggatggt tattatcaca agcacaacgg cggggagatg
    34501 gtgactctgg acagcccggc ttacagcggt agtgatgaga ctttgcttca gaatatccgg
    34561 aagaagcatc tggaacacag gctgaccctt ccggtatcca tgcaggctga attcttgacc
    34621 ggggagaccg caaaattgta tttgtggacc ggagagagtg ccgtcaccgt agaagggaat
    34681 gtggtagaag aagcttcccg gcagcccatc acacaggaaa atatagcaaa acagcttggg
    34741 aaacttggcg acagtcactt ccatctggag gactccatcg gaatccgggt aagtgacaat
    34801 gctttctacc cattaaaagc tatcaacgag ttacgccgaa agggactttc cctgctggaa
    34861 gaacaagtga tccttgcaaa cggcttcctt tatgagagaa cttccgaaaa agctccgaca
    34921 ctggctgacg atggtgattt tcggaaaaac gatagtataa agtctcaaaa atccgatgaa
    34981 aaatgggctg tcacgatacg gaccccggaa caatggcagg gcttttgcag ttcatccttc
    35041 tggaaagatc cggtcagaac cgccggacaa ctcagactct atctggatgc ggatttcctg
    35101 ttacaggatc cgtccgctgc ggagaagatt ttgagaagcc ttactaaaaa agagatccaa
    35161 tctgtgatcg ctctgccgaa gatcctgcgc ctgcgggatg ggcagtatct tgaaaaatta
    35221 cggaaattgc tgctggaaaa tctggcatat ataaatggct ttcaggcagc aaacatggaa
    35281 catatcgcat tattaaaaca atggaatttt accggaaaag aaatctatgg ggatcatagt
    35341 ctgtatctct ggaaccggac cagccgggat ttctggaagg catttttaga cggctattgt
    35401 ctgccgttgg agctgaatgc tgcagagcag agggacattc tggatcctgc gtttcctgcg
    35461 gaaaaagtaa tatacggcag gatccctatg atggtcaccg ccaactgcgt gcagaagacc
    35521 actgaccggt gtcagccgca ggagaatccg aaggcattgg atctgatcga ccgctatcat
    35581 aaaagatttc cggttcagcg gaattgtacc cactgtttta atgtgatcta taacagtgtt
    35641 ccgctttcct tgcacaagga attatgtaaa tggagcggcc tggtgaccgg acggctggat
    35701 tttaccacgg aaaatgagac cgagactttg gaagtcctgg agtattttgc cggtacccgt
    35761 aaggaattgc cctatgggga atataccacc ggtcatgaaa aacgtggagt agaatagaca
    35821 gaaaggtttt ggatccatgc aggaatatgt gattgaatta tccaaatatt ttatcgcctt
    35881 ttttatggtg ttatatacgg caagctgttt ttataccttt cggtatccgg tgggagagaa
    35941 gcatcgtgga gtatttattt tacaggatat tttgatgttt ctggtgcagg tcctctgttt
    36001 tctgaacctg tcccttgtca gcggagattt tcagtatttg tttttctttg catttatact
    36061 gatctttctg tttgctacga tcacgatggt atcgctgatc tacgagaata ttaacaggct
    36121 tttgctgaat aatatgtgta tgcttctggg aatcggtctt tgcatcgtat cgagattatc
    36181 ttttgacaag gccatccggc aatatgtgat tgtactggta tctctgatct tttccctgct
    36241 ggttccgtat ctgctgggaa agatccactt ttttaagaag atcacctggc tgtatgccac
    36301 gttgggaatt gcgctgctca gtacggtgct gatcctggga gaggtgaccc atggttccaa
    36361 gatttcattt tccctgggag gaatcacgtt ccagccttcg gaattcgtca agatcctgtt
    36421 cctgttcttt ctggcgggag cgttgtggga aaatgtctcc ttccagagag tcgttctgac
    36481 ggcgatcatt gcgggagccc atgtggtgat cctggtagta tccaaggacc ttggaagtgc
    36541 tttgatcttt ttcgtgggct ttgtgtttgt ggtttttgct gccacaagaa attatctgta
    36601 tcttctggca ggacttgtcg gcggaggtgc ggcttcttat ctggcttatc agctgtttga
    36661 tcatgtacgg gtcagagtac tggcctggca ggatccctgg aaatacattg acaataaagg
    36721 ctatcagatc acccagtcat tatttgccat cggcagtgga agctggttcg gcatgggact
    36781 gttaaaaggt aatcccacag cgattcctta tgtggaagcg gactttatct tttcttccat
    36841 ctgcgaggaa ctgggcgtga tcttcggaat atgtctgatc ctgatctgtg tcagcagttt
    36901 tctggaaatg atgcgggtgg cagtatgcat tcatgacaga ttctatcagc tgatcgtata
    36961 cggcatcggt attatgtata ttttccagat attcctgacc attggcggcg gtaccaagtt
    37021 cattccactg accggagtga cacttccttt catcagctat ggaggaagtt ccgtcatgac
    37081 gaccatgatc atgtttttca ttatacaggg aatctatatt cgcctgcaga aggaaggaga
    37141 gcgcagaggt ggcagaaaat aaggcgcaga atacgcagaa aaatacacag aaaaaagatg
    37201 ccgaccgcaa aaaggacgcc cggaacagaa aacgtatggg caaccagcgg gaaatctggc
    37261 tttctgctgg aagcatcggt gtcctgttct tgtgcctgtt ggtctatctg ggtcattttg
    37321 tggcaaccag cgaacaggat atgatcaaca acagttataa ttcccgtcag cagattctgc
    37381 tgtcccgcaa ttaccgcgga tccatttatt ccagggatgg ggatgttctg gcagagacga
    37441 tactggatgc ggatgaagag gagactcgaa actatccgta taagaatctg ttttctcata
    37501 tcgtaggtta ttccacccag ggacgcatgg gagtggaggc tctggccaat tactatctga
    37561 tcaacaccaa tacctccctg agcaataaag tgaaaaacga tactgccggg aaaaagaatc
    37621 ccggagataa tgtatatacc acactggatg tccatatcca gcaggtggcc aacgaccagc
    37681 tggatattta ccgtggtgcc atcattgtaa cggaagtgtc taccggcaag attctggcaa
    37741 tggtgtcgca tcccgatttt gatccgaata ccattccgga gatctgggat gatctgatcg
    37801 acaatgattc cagtacagta ctgttgaacc gtgccaccca gggactttat ccgcccggat
    37861 ccaccttcaa gatcgtaacg gcattggaat atatccggga gaatcccacc acgtatcaga
    37921 attattctta taattgtaat ggttctttcc gggtgggaga cagcaaaatc agttgttatc
    37981 acggttccag tcacggacag gtcagctttc agaagtcctt tgcaaaatcc tgtaactcgt
    38041 cctttgccaa tattggaatg agcctggaca gagaggcttt tcaggagact ctgaatgacc
    38101 ttttgttcaa taaagatctg ccacttacgc tgaattatgc caaaagctcg gctatcgtct
    38161 ccgaaagtac accttccgcg gaaatgatgc agacctccat cggtcagggt aagacccaga
    38221 tcacaccgat gcatctgaat atgatcacct gtgccattgc caacgatggt gttctgatga
    38281 agccctatgt cattgatcat gtggaaaatg atgaaggtac tgtggtaaaa agctttaagg
    38341 catcggaata cggccgtctg atgacagagg acgaatctgc gattctgaaa cagttgatga
    38401 ccgatgttgt ggaagaggga actgcttcca agttaagagg tttaaactat acggctgccg
    38461 gtaagacagg ttctgcggaa tacaataata tcaagggtga cagccatgcc tggtttaccg
    38521 gttttgctcc cgcagaagac ccggaagtct gtgtcaccat tatcgtagag ggtgcaggaa
    38581 gtggcggtga ttacgcagtt cccattgcaa gacgtctgtt tgatgcttac ttcaaccaga
    38641 aagagtgatc ggaggagtca aaagaatcat gatagaggct accagttgcg gtggcgttgt
    38701 gatcttccgt gggaagatcc tgctgctgta taagaacatt aagaataaat atgaaggctg
    38761 ggtattgccg aagggtaccg tcgaagccgg ggagacccat gaggagaccg cactcaggga
    38821 agtgctggag gaaagcggtt ccaaggcata cattgtagat tatatcggca gaagcgagta
    38881 ttcttttacc gtaccggaag atgtggtgga gaaggaagtc cactggtatc tgatgggaac
    38941 ggacagctat tacagcaagc cccagagaga agagtatttt gtggattccg gcttttacaa
    39001 atatcatgaa gcatatcatc ttctgaaatt tgccaatgaa aagcagattc tggaaaaagc
    39061 ctatgcggat tatcaggaaa gaaaaaggaa taatcaatgg aaaaattgat tattcctgct
    39121 tttctgaaat atttttaaac attcttgacg atttccagat ctggtctctg caagagatct
    39181 acaaattcgt ttttatcatt ttttaaaatg ggacgggagt atttgttggg atgaatgatc
    39241 agtacttccc agatgcttcc gtcattcagt tccacgctgg ttccgatctg ggcatctgca
    39301 atacgtttca tgatcggcat caataattcc acgtcatatt tatccagact cttttcgaaa
    39361 ttcccaagaa tctgtaacgg tgtcaacgca ttacggtagg ttctgggaga cgccatggca
    39421 atataggcat caataatggc aatatacctt gcgaagacat caattctctg tccggacata
    39481 tggtaaggat agccgctgcc atcgagtcgt tcgtgatgca tgatcacagc atttttcaca
    39541 tgctcattca gatcctgatt acgtacggtg gtataaccga ctacaggatg agtcttgata
    39601 actttgaatt cttcatccgt cagtttgccg ggcttccata acagttcata gggcagctgc
    39661 agcttgccga tatcataata gaaaccgcag agaatcaggg tatttttctc ttcttcgctc
    39721 attcccagcc agtcagcgaa ggtccccgcc agaagagcgg cattcagtcc ctgggtatag
    39781 gtgagttcat cctcactggg catcagattg tacagatagt ctaacagtac cgggccggaa
    39841 gtaatataat aatacaattc gttgtaaatt gtcagcagaa taatatccgc cggttttctt
    39901 cccatgatca gcagttgttt catggctaat ttatattgtc ccaaaaacaa cggataagca
    39961 cgttcgaatg ctttgaaatt agaatcaaaa cgaatcttag cataatgtgt agtggcgaaa
    40021 tcaacatctt ccattatggt gacacataca agattgtacc gtttcaattt ctcaatgatc
    40081 tgcggagtga tggtggttcc tgcagggtag atcacacgat cctgatccag aatgtcctcc
    40141 cccaggacca tatccggctg taactccatt ttcgtaataa ccttcaaagc tgtatcctcc
    40201 tcattcgtac taattcaagt ataaaatatc atgctaaaaa gtcaagtaac aaaattgtaa
    40261 tataaaggaa actatgctat actaggagaa aaagatttaa ggaacttgtg tatggcagga
    40321 aagctgaagt ttgcgcaaaa tctctatctt ggagagggca tcgcgccgga aaaactggat
    40381 aaactaaaaa agaggctgaa taaaaagcct cttctggcca atgtatatct gatcactccg
    40441 gccagaaatc ccgcggatca gctggatatt tttgatgcca gacagttggt acagcctcat
    40501 tataaggacg aagaatttct cgttctcggt atggcttccg gctacgagga tgcgctgcag
    40561 ctgatcgaac ggatcaccgg agagtgcctg aaagccagag gggactgtaa tctgcgggag
    40621 tatttactat gttgacgatt ctttttaaaa tattgagtat tcttgggatc gtgtttctga
    40681 tcctgttggg aatcgcactg gtggtaattc tcctggttct gttctttccg atttcttaca
    40741 aagctgccgg aaagcggacg gcagaggaat tctttatcac cgcgagagta aaatggctgt
    40801 tcggtctatt gcggatgaac tgcgattatc ctgatcccgg gaaaatttgc gtaaagtttt
    40861 tattctttac gctatatgat tcttccgtag agaagccgga gaaaacagac cggacatcga
    40921 cggaaacgac agggaacgag aaaacacccg cttctgcgga gcagacaaca gaagaacata
    40981 ccccggaatc tgtcgggacg cctaagctga tcgaagcaga agatcccacg gcattctcgg
    41041 caacggccgc agatgttgag tctgctgcgg aaagggagga ttccagaatc ccggaaccgg
    41101 gaacgccgga gccagcggga gaagctcccg ggaagattac ggatcgtctg tacggatttt
    41161 ttacaagaat ccggtacaca gtccgaaaga tttgtgataa aatcaaaaat attctggaaa
    41221 acattgcatt ttacaagaac ctgtggaatg acccggatac ccagggaatt ttgaaacatg
    41281 ccgggaaacg tttcggacat atctggaagc ggctgcgccc gagaaagctt acggtcaatg
    41341 ccacggtcgg caccggttcc ccggatacta cgggatatct gtatggtatc tacggaatgg
    41401 tgcttccgaa gctgggaaag gggatctgca ttaccccgga ttttgaacag gcgattctgg
    41461 aaggggattt caaggcttcc ggacatttta ccgcggcctg tgtattattc cattccgtcc
    41521 gactgctttt agatcggcga ttacgggaat tgttgcataa aatcagacaa tatcagaaga
    41581 cacagaaaaa ataacagtgt aaaacaggag gtttgttatg gcagacaaag agaatcagtt
    41641 tcagggcgtt gtagaatccc tgttgaaggg tatggatacc gtgttatcca ctaaaaccgt
    41701 agtcggtgag gctacaaaga tcggcgatac gatcatcctg ccacttgtgg atgtcacctt
    41761 tggtgtcggt gccggagccg gcaataattc cgagaaaaaa tccgcatccg gagccggagg
    41821 cttaggcggt aagatgaccc ccagtgcggt tctggtcatc aaaaacggtt ccaccaagct
    41881 ggtaaatatc aagaatcagg atacagtcac caagattctg gatatgattc ccgatatcgt
    41941 agataaattt acccagccga aggaaaaaga agaaatgtcc gatgcggaag tggtagatat
    42001 cgcatttcct gaagaggaag agaaaactac agaagacaag taatatttaa aatactctgc
    42061 atataataaa gtgagaacag tctggatccg tttgatagat gaagaagatc attggtctga
    42121 tactcttttt tgtggctgtc ggcatgctgc tcatgcttgt gatccataac cgttttatcg
    42181 gcttgatcat ggtagcactt ttattatttg cagggtatta tcttttctgt ggttgcgagt
    42241 aagagggtgc agtatgattg attgggaaga ggtcattaag gcagagagta ccgaagaact
    42301 gaaagaaata aaactgtggc ttttccagga aaatatgcgt ctgaaacagg aacgctcaga
    42361 gctggagcag gagcgtacgg aactggaaca ggcccggaac aaatttctgg atgagcgggt
    42421 gaaactgcgg gacgagctgg atgaattaaa ccgccgtaca atcatggaac ggaaacggct
    42481 gaaggaagag aatctgtttt ttgagaagaa aatggccatt ttacaggagg ggttccgaca
    42541 gctggaagaa gaccggaaga agttcgaaca ggaacggcag atactcatag aggaagccag
    42601 agaacagcag gccacagggc aggctcccgg agaaggcagt tttacagcgg tacagctttt
    42661 attccgggga gtcaataatc cgctggcttt acggaagaga taccgggatc tgatcaagat
    42721 ctttcatccg gacaacctgt tcggtgacgg tgaactggcc ggacagatca acaaggaata
    42781 tctgaaacgg aaacaggaag agcggttctg gtaactgcat ggaaaattac aagcaaaaaa
    42841 ttaagccaga aatgatgaat ttcatttctg gctttttaaa atccaatatt atcgcagaag
    42901 attaagctct ttcaacttta ccagatttta agcagctggt gcagacatgc attcttctag
    42961 ctccgccgtt aaccttgcac ttaacgctct taacattaga gttccaaatt gcgttgcttc
    43021 ttctatgaga atggcttaca ttgttaccga aatgaacgcc cttaccgcaa atatcacatt
    43081 tagccatctt gacacctcct cgatctaacg cgcatcttac gcttactttt aaacaaattt
    43141 tttcaataaa atacatgtga aatgctcaca acattgatat attaacagaa aaccaaaata
    43201 aaagcaagca aaaaatattt atttttttta ttttcttgtt tctttttcaa aggaaagctg
    43261 ttaaaataca tttatatatg tctgaactgt agattttcag gcagacatat cggaagaaac
    43321 aagtgatccg aatatgaagg aggttctata tgaaaggttc ttctatgaat acccatatgg
    43381 gaaatattgt cattgataat gaagtcattg cacagtatgc cggcagcgtg gcggtagagt
    43441 gctttggtat cgtaggtatg gccggagtca gcatgaagga tggtctggta aagctgctta
    43501 agatggacag catcaccaga ggcatcaatg tgtccctgga caacaataaa ctggttctgg
    43561 acttccacgt gatcgtagct tacggcgtca gcattctggc tgtcaccgac aatctgatca
    43621 gcaacgtaaa atacaaagtg gaagagttta ccggtattga gatcaaaaag atcaacatct
    43681 ttgttgaagg tgtaagagtg attgattaag gaggaagtaa ttgtggcttt acaacaaatc
    43741 gacgctaaga tgctttccaa gatgttctta gccggcgcca agaatttaga gaataaaaag
    43801 gaatggatca acgaactgaa cgtattcccc gttcctgacg gagataccgg aaccaatatg
    43861 accatgacga tcatgtctgc tgcaagagaa gtgtccgcac tgggcgagga tgcagatatg
    43921 gctgccctgt gcaaggctat ttccagcggt tccctgcgtg gtgccagagg taactccggt
    43981 gttatcctgt cccagctgtt ccgtggcttt accaagagca tcaaggaaga gcaggttctg
    44041 gatattcctg ttctgacccg tgcatttgat aaagcggtgg agaccgcata caaggctgtt
    44101 atgaagccga aggaaggtac gatccttacg gtagcaagag gtgcttccga taaggcaaag
    44161 gagctgtgtg aagatggtgc agaggatctg gagcagtttt taggatctgt gatcgaacat
    44221 gcccgttacg tactgagcca gacgcctgag atgctgcccg tattaaagca ggcaggcgta
    44281 gtggattccg gcggacaggg tctggtagaa gttttaagtg gtgcgtatga cgcattctta
    44341 ggtaaggaga tcgatctgac cgtagctccc gcagctactg cgaaggcaga ggataccaag
    44401 agttctctgg aagtacaggc agaggcagag atcaagttcg gttattgcac cgaattcatc
    44461 attctgttaa ataagccttt caacgtaaaa gcagagatgg atttcaaggc atttttagag
    44521 tccattggtg attctattgt atgtgttgcg gatgacgatg tggtcaaggt acatgtacat
    44581 accaatgatc ccggtctggc tatccagaag gcattgaagt atggtgcatt atccaacatg
    44641 aagatcgaca atatgcgctt agagcatcag gagaagctgt tcaagctctc cgaaaaagaa
    44701 gcagcgcaga agaaagcgga agaagagaag gctgcacagc ctcccaagga agtaggattt
    44761 ctggcagtct ctgtaggtga cggcctgagt gaactgttca agagcctcgg tgtggattat
    44821 atcatcgagg gcggacagac catgaacccc agtacggctg atattttaga tgcagtggac
    44881 aaggtaaatg caaagaccat ttttgtcctt ccgaataata aaaacatcat tctggcagcc
    44941 aatcaggcag cggaactgat gactgacaag gagcttttag tcattcccac caagaccatt
    45001 cctcagggta tcactgctgt gatcaacttt gttcctgagc tgtctgtgga agagaatgaa
    45061 gagaccatgc tccgtgagat caagaatgtc aagaccggcc aggttaccta tgcggtacgt
    45121 gataccgtga tcgatgacaa ggagatcaag aaggatgact tcatgggtat cggtgatcag
    45181 ggtattgtag ccgttggcac cgatatggta aaggtgaccc gtgatatgat cgccgagctg
    45241 gtagatgagg attccgaact gatcagcgta tactatggct gtgatgtagc agaagatgct
    45301 gccgaagcac tgcgtgcaga tctggaagag gcttatcctg catgcgatat tgaactgcag
    45361 tatggcggac aacctattta ttactatact gtttctgtag agtaaat
//
LOCUS       DAWEJW010000008        42607 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_407_length_42607_cov_53.574662, whole genome shotgun sequence.
ACCESSION   DAWEJW010000008 DAWEJW010000000
VERSION     DAWEJW010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 42607)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 42607)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..42607
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_407_length_42607_cov_53.574662"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5918..6017
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12209..12308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23858..23957
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    29868..29967
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        11995 a         8326 c        11336 g        10550 t
ORIGIN      
        1 aggccgtggc cgtggaccag ttcccctgga ccgcaaatgt cgagacggtt gtcttacttt
       61 ccaagggcgc taaaggtccg gtggaccttt gctcagcgcg gaccgaagtg gaacggagct
      121 gatcaaggtt atgaccggta tccgccgctg cggaaagtcg gtcatgctgg agctgattca
      181 tccgcaggaa ttacaggata agcattattt ccgtaagcat ggtgcaaatg catattacgg
      241 acagaattaa caggatatcg agagggtata aaacagatga agatgaaaac agcacctatg
      301 gggtggaaca gctgggattg ttatggagcg gctgttacag aagatatcgt acgacggaac
      361 gctgaattta tggctgagca tctgaaggag tacggttggg aatatattgt ggtggatatc
      421 caatggtatg agccttatgc tgcaaccaat gagtatcatc cttttgcgga tgtagtaatg
      481 gatgagtatg gaagactgct tcccgcagta aatcgttttc cttccgcagc aaacggtgcg
      541 ggctttggac cattggcaga atatgtccat tctcttggat tgaagttcgg catccatatt
      601 atgagaggga ttccacgaca ggctgtacat cagaacacaa aaatcatgaa cagtgacaga
      661 catgcccggg aaattgccaa aacaaacagt atctgtgcct ggaatacgga tatgtatggt
      721 gtagatcctg agaaagacgg ggcacgggaa tactataaca gtatttttga attatatgct
      781 tcctggggtg tggattttat taaatgtgat gatattgcac gggaactgcc acatgaggaa
      841 tccgaactga ttatgctgag caaagcattg catggttgcg gaagacccat ggttttaagt
      901 ctgtcgccgg gacctgctct tttagaaaag gcagagcttt ataagcagat ttccaatatg
      961 tggagaatca ccgatgattt ctgggataag tgggaactgt tatatgatat gttttcaaga
     1021 gcagaaaaat ggtgcaccca tgcaggtgca gggcactggc cggatgccga tatgcttccg
     1081 gtcggaccga tacggcaggt atatgatgtg aataactgga cgaatttcac acaggatgag
     1141 cagatcacca tgctgacact ctggagtatc atgcgttctc ctttgatgct gggaggagag
     1201 ctgacagggt ttgacgaatt cactatgaat ctggtaacca attctgaaat acttgccatg
     1261 catgcaaatg cacggcattc gcatcaggtg tggagacgtg aaatcgatgg tatagagcat
     1321 gcattgtgga ttgcagcaga tacaaagggt ggatattatg tcgcggtatt taatctgggt
     1381 gataaagaca gtgacatatc cataccgctt gccgatctgg agatttatga tggagtaaat
     1441 ggaacggagc tttggagcgg agagcacatg gaagagccaa agagccttag tgtgtcatta
     1501 aagagtcatg gtgctagggc atatcatttt acgtacaact aaaaagcata ctactgttaa
     1561 tgtatagaga tcttcaatca tgatctcagc tttgagatta accttatagt cattttcagc
     1621 acgttggcga tctttttctt cttgccggtt ctgactcatc atgataagag gtgcctgaat
     1681 cgcagctaca caggacagca cgagattaag caggataaaa ggaaatggat caaaggcttt
     1741 gaccgcaagc agattcagga cctcctcatt ggtcatatca tggcgcgaat ttctttaagc
     1801 acttctttta atagtttgcg gttttccttc atgctggtct ccttctgttt attcccggta
     1861 aatctgtgca aaccggaaat ttctgatgaa aaacatggtg caggatcgtt ttcgattatt
     1921 atattttact gtgagattgt gatattagca atagtgaaat ggggcagaga agagttgaaa
     1981 aggcagacgg ataagaaaca aagataaaat agtaaaaata gaatagaaca aagaaagaat
     2041 atggagataa aacagaaagg aataaacaat gaatgtaaat cctataacaa gattggatta
     2101 tccagatccg gacgtgatcc gcgtagaaga cacctattat atggtatcca caaccatgca
     2161 ctttatgccg ggctgtgaga ttctgcagtc ttttgatctg gtgcactggg agcatgtgac
     2221 ttatgtctat gagacgttgg atcatacgga tgcgcagcaa ctgaaaagcg gtcagaatat
     2281 ttacggacag ggcatgtggg cagcttccct gcgctatcat gaaggccgat tttgtatctg
     2341 ctttgtggca aatgataccc gtaagactta tttgtatact tcggaaaaaa tagatggtcc
     2401 atggaaaaag cagctgatag aaggctttta tcatgacggc tctttattat ttgatgagga
     2461 tggcagaaac tatattgtat atggaaatga tgaaatctgg attacagaat tgaatgaaga
     2521 tttgactgca ccaaagaagg acggattaca tcgtctttta gtcagtgatc ataagaaccc
     2581 ggagctaggc tatgagggtt ctcatattca aaaaataaac ggctattatt atatttttct
     2641 ggttcattcc ctgagagacc gctggatgcg gacggaagca tgcttttaca gtgattctct
     2701 ggaaggagaa ttcacgggtg gagatgtact ttgtgatgat cgtggatatt gtggacaggg
     2761 cgtggcacag ggaggaatag tggatacgcc ggatggaaag tggtatgcaa tgctgtttca
     2821 ggattccgga gcagttggac ggattcctat tctgcttccg gttacctgga aggatcattt
     2881 tccgtttttt ggacagaacg gacatgtgcc ggaagaaata gaggttcaca gtaccagacc
     2941 aggatatatt tatcagccat tagtgggaag tgatgatttc tgtaatgggc tgcttccgcg
     3001 ctggcagttt aatcatgatc cagatccacg gtactatcat ctggatgcat tgcagggaac
     3061 ctatacaatt acaacacggg aagtttgtac agagcttaca caggcagtaa atacgcttac
     3121 gcaaaggatg agttatccgt cctgcgcagc tgaggttact gttgatgctt ctgcattgaa
     3181 agagggcgat gtcgcaggtt tgtgtgcgtt gcagagctgt tatgctgcag ttggcattac
     3241 aaaacatcat ggaaaatatg aagtccttat gctggataaa aaagaagatg gtatattgga
     3301 tatgacggaa ggaactgttg ttgaatcaca ccagatggat tttcgtcttg aagcagattt
     3361 ctgtaacatg aaggatgaag cacgctgcta ttaccgggta aataagggag actggctacc
     3421 catcggaacc gtgcataagc tgtacttcaa actggatcat tttactggtt gccggtttgg
     3481 tctgttctgc tatgcggcag aggaaacagg aggaagtgcc tgcttccggg attttaagta
     3541 ttatgatgtt gatgaaatct cataaagata gaactgcata aaataagtgt cggaatttac
     3601 tcaaattttt cctactttgg acattggttt ttacaacggt attgtatagt gaagcagagg
     3661 aaaaacaaaa catgtttcag agaggagaaa cgattatgag aattaagagt atgatgaagg
     3721 gtatgcttgc tgttgtaatg gcaatgtcta tggcagcatg ctccggagca cctgcagcag
     3781 accagacttc ttctgagtca ccgtcaccgg cacagacaga aagtacagca acagagcagg
     3841 aaacaatcgt aacagaagta tctgcagaaa cgcagacgca agaagaaaca gcaaaagcac
     3901 cgaaatatgt tttctgttta ttggtgatgg tatgagctat ccccagtttc agacagcatc
     3961 tgactatctt ggagcaatag cagatgaaga ttatgtcaag gcacttccga gcaatagcta
     4021 tgaagaccgt ggaggagcag tactggacgg accggaagca ggatacaagg ttacctatac
     4081 acaggctgat gcagaagcgg ttacggctga agatggaagg gttattctga tggatgagca
     4141 tctggcagat tccgacgcta tggattatga tatggatcgt gcagatggag aatgggcatt
     4201 atctgactat gtacaaaagg gtattgaagt attggataat gacaccggat tctttatgat
     4261 gtgtgaaggt ggaaagattg actgggcatg ttttcagccc agtcccggca gatttccgtc
     4321 atcagcagac ggaagcggat ttacacacct gcttcttatg tacatgaact tggattaaaa
     4381 ttcggtattc atattatggg aaggaagagt ttcatttaat cagatgatga aggatattaa
     4441 gtcaggaaaa gatacggatt ttgttgacga gcttattttt aaggtcataa atgcaccgac
     4501 caagagaatg aaaattgaat atgtctaagg caaattacaa agccgcttat tcttattgat
     4561 tttaagagta ggcggttttt tgcgttctct tgtacttgtt tacatagcac cttgacaaag
     4621 tggctaaaac tatgcgaaag gaggacgcat ctatgtcaaa tcgcaaaaag acggtgttgc
     4681 cgacagagat tgtgacagcc gtggaggact gcggcttttt tgaaagcatt cccctatggg
     4741 cggtgacgct catcggcggg ctgttcatca cagtgctgtc gttcattatg ataatgtcgg
     4801 tgtacggcag gtttttcaag ctgtaccttt ataccgcaat cgctcccgtc cctctttctt
     4861 ctttgcggga gagccgagcc agaatgtcgg caggagcttt atcaagagtt attgtgccgt
     4921 gtgccttgag ggtgcaatca tcgtgcttgc ctgcattatc ttttcggtgt ttgcggcttc
     4981 cccgcccgtg gtcaatcccg atgcggctgc ggtcacgatg gtgtggagct atatcgggga
     5041 actggtattc aatatgctcg tccttgtcgg tgcggttaag atggcagacc gtgtcgtgcg
     5101 tgaaatgatg ggcttgtaag ggagaaagcg gatgaaacgt gtatatatat ttaaggacgg
     5161 ggtgcaaaat gcttcgttgt cgatagattt ggattacaac cttgagattg taagatgtga
     5221 agattttgag gatcgcagaa atctaaaaga atgtgcgagg aaatccttta ataaggcgtt
     5281 aaacgagcgt gatttaggag attgtgagga ttcaacatct tctctgacta cgggggaaat
     5341 tcattttgtt agaggaaatc ctaccgagtt ttcaatggat gtctgcattg tatgcagaga
     5401 tacagaggaa gatttttaca gacttattca caaaaaaaca gggttcactt atcgggatga
     5461 atactattgg aacaaagccc ctcattccgc aggaatacag aaaaaggcta agtatattaa
     5521 gaaaagggga aaatggcagc ttgtcagaac gcagtatttg aatatcaaaa acagatacct
     5581 gcggcagaac gaccacgacc atccctcttt catatgctat attgaagctg taaacaatgt
     5641 atataacgca agaatgagtt ggaagtaaac ggagtaaaac ttgaagaatc gtcatttttc
     5701 caaagatggc gatttttttg tgctaaaaag gaggtgttta tggattggaa gtaaagataa
     5761 ataaagaaat ccgtaattac acggaatcca tgtttttcgg gctgtctatg cggcagttcc
     5821 ttttttccgc ccttgcctgc ggcatacttc tatgcccaga aagcagggca aaattaagaa
     5881 aacaccctgc gggtatccct ttatgcccaa gatgcagnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnngca gggcaaaatt aagaaaacac cctgcgggta tccctttatg
     6061 cccaagatgc agggcgagta attgactttg accatcgccc ctttaaaaat gtggaagtca
     6121 tggatgaggc aatgatatcc ttatggaatc aaagagtaaa tcatgatgac aatgtgtata
     6181 ttttggggga tttctgctat agaaacgaaa aaagtgcgga atggtatctg agtcaactaa
     6241 agggacataa gcatctggtt cttggtaacc atgataccag gctgctggat aatgagattg
     6301 caatgggata ttgtgctcca tcgataggat gatgatgtac gtgtcggata gtgggaaaga
     6361 gatatgtctg tgccatttat ccgatgtcgg aatggaatgg ctcttacaga ggtcacttgc
     6421 acttttacgg tcatattcac gataagaggg atgaaacatg ggaatatatg aatcagaggg
     6481 agaacgctta taatgtgggc tgcatgttat atggatacag accggtacgc ctgaatgaga
     6541 ttgtgaaggt gaacaggact ataggattgt ctgcggagac tttggtcttt gcgtaagcgg
     6601 cggatgttcg aagaatatag tgcccaaaaa ggaagttcta tgttataatt caaataacat
     6661 caaggacttc gcaacacaca gagcagatgt aaggtggcat gatgagtaaa aatatatgca
     6721 gattagatgg aaatcaaatt tttttatccg tactgaggga tgatgaggag gcagtcggct
     6781 tatacgcgaa gtggatgtcg gatgaaacca cctgtgtctt tatagagcat gcgcattctg
     6841 tggtggacat cacccggatg cccggttggg taagagacca ttctgtcatg cggatgggaa
     6901 ttgtccttaa agagacagac acattgattg gatattgtca tattgaccat tgaactgagg
     6961 atatgtcagc ctggttatcc attaacatcg gagaaaagtc ggtgcgtgga aaaggcattg
     7021 gaaccgaagt cgtgcagata ctcttgcggt actgctttat ggtgttggga gtgtatagtg
     7081 tccatctgga tgtactggaa acgaacaccc ctgccattaa gtgttacgag aaggcaggat
     7141 tcagaataag tggcagatac cgggggcatt gttactatat gcggaaccac tatgaccggc
     7201 tgcacatgga tatcttatat gatgaatacc tgaaaataca tgtttagtgg aacatatcct
     7261 atttctttca acaattagat gatgaaatat gtgaaaatgt gagtatttgt gtggtgcctt
     7321 cccataccaa aggagagacg aatgagtctg gaattgctga attagcacgc agattggcac
     7381 agaacaatcg tatagataaa gttaattatc tgatcagaaa caaaacaatt gataaattgg
     7441 catatggcgg aagtcgtgat attagagtgc aaatcggaag cataggggtt aatcctgata
     7501 tgacggtaga aggagataga caagcataag gcaagaggag tgagagatac aaaagaactt
     7561 atggaatttg taggacaacc ggagtatgaa taaagcaaaa aaagtccgtc ggagcgttaa
     7621 agcatcgatg gacttttgct ttgctatttt acaaaagtaa aaatcaaaaa attacagagt
     7681 ctgatgcacc tcgcggatca cggcttcgct aagttccgga gctttgtcgg gatcgatgtc
     7741 atcgggaaat tccacatcaa tggtggaagc aaccggccgg tagttcagac gcagtgcgtt
     7801 tttctgaata taatcggtag cctgattgta agtttcctct gtaacgccat ttaaagtgat
     7861 cgtatataat tttgccataa tctatcatcc tttctgttgg tttatgagtt tgtggaaaca
     7921 tttccttgtt tctgtatata gtatatctct ttttataaaa aaaagaaatg cacgaaaagc
     7981 ataaatataa taataggaat cattactata tttgttaaat atatacaata tatcaaatag
     8041 aaaagaactt acaatgattg acagaaagtg taaatttcaa tatagtggaa gaaatggaaa
     8101 taaatgtatg gaggcatctt acgattgaat attgcaaagg ataaatggat gaaatacaaa
     8161 ggaacttcgg gcagagcggt tctgactctg tgcggtgtct acggggtggc atatctgttc
     8221 agcttacagc cggtgaaccg gatctattac agtgttctga atgtgctgct ggctgtggga
     8281 atctattcta tgttgcagca gacggagcaa aatctccgga aaatagagga gaaaagggac
     8341 aggaagcgca gaatctatta tgtggcagtg atcagcttcc tgtttgcctt gtccatggtc
     8401 atgggatatc agttgcagaa tctgggaatg acggattgcg gagtccgggg caaagggatg
     8461 atcctgatcc gtgcgctgtg cttaagtgtt gccgcatttc cttttgcaaa tctgttgctt
     8521 gacgggatag accgactctc tgcgagagga gaagcaacga tgtcggggag atattggaag
     8581 tccggcattg tattcgcagt aacggcagtc gttattttcc tgtgcctgat tccggtatgg
     8641 ctggcttatt acccgatcat catgtcttac gattttcaca gacaggtcaa cgaagcgtat
     8701 aagggcctgg aatggtttta tcccttgcag cccattgcac atacatggct gatccggctg
     8761 tttttacaga ttggaacggc catcggatcc tacgaggcgg gaatagcctg tatggcggta
     8821 ttccagatgg tgctgtatgc tctggtcatg ggttatgcaa cgtccatgat ctggcggatg
     8881 actgctaaaa aatggccggt ggtggtaaca acactatttc tggcgctttt cccatacaac
     8941 acggttttag tggtgtgtac cacaaaggat accttgttta ccatattgtt tacgttgttc
     9001 tttttacttt ttctggagag aaataatttc agcaacggaa aaaagaaaat cctcatgaac
     9061 attcttctgg tggctgaggg ctgtctgatg atgcagtttc gtaataatgc agtatatgcg
     9121 gtggcggtat ttatgctgct gttattgatc ctgcggccga aaaaggaaaa actgggtatt
     9181 ctgattctgg ggatttgtct ggtactggga gagaccggaa tgcgaaatgt gatccagaca
     9241 gccataggga cccggctgga ggctccgaag atcgaggctt acagcgttcc catccagcag
     9301 tttgccagag tggcatatta tcatggagag gaactggagg cgcaggatcc ggagctggcg
     9361 gcattgctgg aaaaatatgt gccgagagat cattgggatg cttattatgc gccgctggcg
     9421 gataccatca agggcgcggt aaatgcttcg gcatatacgg agaattccag gcagtttttg
     9481 agagactggc tacgagtggg caaacggtat ccgaacgagt tcctggatgc ttttctggaa
     9541 ctgaccagag gatactggtt ctgggatgat ttctcctggg cggaaaatct gggctatggc
     9601 acagacacaa ggtatggtgt gctctttacc tatacttcct cggaaatcga aggctatggc
     9661 agtattgaac atcggtcgaa attcccggca ctggaaaaag tgctggaaaa gatcgtcagt
     9721 ggtaactgct ttaatgaatg gccggtgttt tccgttattt ttcatggatc tctatacagc
     9781 tggagcctgt tctttttgat ggtgttgtgc ctgtatcgaa gaaaataccg gtgcttccag
     9841 atgagcttac tgccgttttt atactttggg actatgttgc tggggccggt agtgcaggta
     9901 cgttatttat tccccattat ggtaatgctg ccggtacttg cggcattttt ctcgagtgac
     9961 aggatgaaga gccaaagcaa tgaaaacagg gaacaaaagg ttgtttttct gggccggaaa
    10021 atctataata aggctattgg atgataatat aaacggaaag aggacccaat ggagaatcag
    10081 gagaagaacg aaacaactgt gcttccggag aatccggaga ttatggtaaa gaatccccgg
    10141 atcggaaagg tacggaaact ggaagaggaa gtcaggcgcc agaacgagct ggagcagctc
    10201 agaaccaaac ggatccgcaa ggagcaggaa tatatcgagc agatgagcaa ggcggtggaa
    10261 gaccacctgc gcaataccag agaggaacag cggtataacg aagaatttga acggctgatc
    10321 cgcaaggatg tctatcggat gcacggcatc agtgaggaca agctgcaggg aatgacggag
    10381 taccgcaatg cctggtatca gggaacggcg ttcggcatgt tttttctgtc gctgattctg
    10441 accggaatct gcggatatct tcatggcttc ggttcggaat tgtgtatttt tatggcattt
    10501 tataccgcta ttgagggaac acttctgacc aatgggaaaa gacagcatgc attgttggat
    10561 atggtgacaa gaattctgta tctgctgttg tttccggcca tgctgatcgt atttgtctgt
    10621 tttgaactgg gatttacgga gtatgggatc gtcgtaccgg tactgacgat tgccggtgtg
    10681 gtgattctgg tgctgggagc ggtatcgtac tttgtgtatg acccataccg cctggaccgt
    10741 aagaaccaca aaaaggcgga ccgctatatc cgggatatag aaaaagccgc tgtcaaggaa
    10801 gtggaactgg aagaaaaggc actggcaaaa caggccagaa aacaggaaaa actggaactt
    10861 aaggaagagg ccagacagga acgggcagca aagcgaaggg aacggaagaa ccggagggat
    10921 gagaagctca ccggctggaa agaaagcatt cgcgaaaagc ttccggcgaa gaagaaagcc
    10981 acagaggaga gcagaacgga gacaggcatc ccaaaagagc cggaagccgt gacggaggaa
    11041 aagccgcagg caggtgcaga ggagtccggg acgccggaga caaccggacc ggtagaaaac
    11101 acagagcctg tagagactgt ggaacagaag gatgcacctg cggaaccgga gcaggggaca
    11161 ccggaagaga ctggtaaagc ggaagaaatt gatacaacaa atggggagga gaaataatgg
    11221 cctattatct gattgatttt gaaaatgtaa agagccgagg catggaggga gtggaactgc
    11281 tggcggagga ggataccgtc tgcattttct acagtgacaa tgcggacagc atgacttttg
    11341 acctgcatcg gaagttgaat gagacgaaag cacagatcat ttaccacaag gtagcagtgg
    11401 gtaccaagaa tgcactggat ttccagctgg caacgtatct cggttacctg atctgtgagc
    11461 agcagaggga agggatacac ccggattact ttatcgtgac caaggacaat ggattcacca
    11521 gtctgatggt atactggaaa gcacagggag ttcccgtgcg cattatccgt aatctcttgt
    11581 ggggaaagaa tccaatggcg gaacaaaatc tcctgaccga agaaaatgca atggaagtaa
    11641 cggaaagtac ggaacaggag agcgtgcagg ctctgtcggt ggaagctgca gagccgatgg
    11701 cagtggaaat cacagaacag gagacagaga acgcaacagc ggtaatgacg gaggaaccga
    11761 aggcggaagt ggatgcagag gagattgcag agccagcatt tgtgagcgtg cagatcctgg
    11821 aagaaccgtt gcctgagata ctggaacctg tgcctgtgga acccgtgaaa aaaacggtaa
    11881 ggaaaaagag cggaaatacc cggaagaaga atactgcaaa tatgacagaa aagaaaacga
    11941 cggagaaaac tgttgataag aaggcttcag agaagaaggc agcagaagaa ccggatgaac
    12001 tggcccaggc agtggaaaag gtgctttccg accaggccat cgtccctcag gtggtaaaat
    12061 tcatccgcca gtacaagacc aaacagggaa tcaacaatgc cctgaacaaa gagttcaagg
    12121 acagcaaacg taccagcgag atttattccg ctatcaaacc tctgatcgcg gataaaaagg
    12181 gaaaatgata aaatagatca acaaaaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnntc aaacctctga tcgcggataa aaagggaaaa tgataaaata gatcaacaaa
    12361 aaattaaaag aaaactattg acattataaa atcagagtgg tatttttgct atcgaacaga
    12421 aagtgaaatt cgatgaacag aacaagatct gcatacggaa gcatttcaga gagccgccgg
    12481 ttggtgtgag acggcatgtg agtatgacag actatcctct gtgagaagtg cactgaagat
    12541 tttaagtagg tgtcaccggt attctaccgt tatttagaag cctgttggaa atgagatatt
    12601 tgtatttcat ggagatggga acaagaggtc atgtgataaa acatggcaag caaagtggta
    12661 acgcggagag cttagagctt ttcgtctttg ggtgcttaag ggcaccggag acggaaagct
    12721 tttttgtttt acgggcaatc agaatttttc tttctgtcac gacaaaacaa caagacacaa
    12781 gaaagaagga aacaaaaatg aacagtttgg taatcattct gatcgcaatg gtagtgctgg
    12841 tagcaggcta tgtcttctac ggaagatggc ttgcaaaaaa gtggggaatc gatgagaagg
    12901 cgaagacgcc tgcgtttacc catgaagacg gagaggactt cgtgccttcc tccaaattca
    12961 ctgtattttc tcatcagttt tcctctatcg ccggagcagg tcctgtgaca ggtcccatcc
    13021 tcgcatcggt attcggatgg gttcccgtgc tcctgtggct tctgatcggc ggtctgttct
    13081 tcggtgccgt acaggatttc ggtgcgctgt atgcctccgt gaagaacgaa ggaaaatcca
    13141 tgggaatgat cattgaaaaa tacattggta agaccggcag aaagttgttc atgctgttct
    13201 gctggctgtt cactctgtta gtcattgcag catttaccga tatggtggca ggtaccttcg
    13261 taggtaccgg actggaagat gccagtgtgg catatgccaa cggcagtgcg gcatccattt
    13321 ccatgttgtt catcgttgta gcagtgatct tcggtctgat ccagaaaaag gtcggcaaga
    13381 tgaacgagtg ggtcaaggca gtcgtagcca tcggtctgtt ggtagtaatg ttcgctgtag
    13441 gtatgcatct tcccatctat gcttccaaga ctgcatggat ctacatcatc atggcatacc
    13501 tgttccttgc atccgtaatg cccatgtggc ttctgatgca gcccagagat tacatgacca
    13561 cctttatgct gctgggtatg atcatcggtg ccgtacttgg tgtggttgtg gctcatcccg
    13621 atatggagct gaatgcattt aacggttttc aggtaggtaa cagcagcctg ttcccgactc
    13681 tgttcgtcac catcgcctgc ggtgcggtat ccggcttcca cagcctggta tcctccggta
    13741 cttcatccaa gaccatcagt aatgaaaaag atatgcccat ggtaggttat ggtgccatga
    13801 tcgtagagag cctgctgggt gtggttgcac tggtagttgt cggtgcagtt gcagtcaatg
    13861 gcacgaagcc cgatggtaca ccgttctcca ttttctcctc cggtgtcgca ggattcctgg
    13921 agaagatggg ggttcccgta accgtagcaa ctgtattcat gaccatgtgt gtatctgcat
    13981 tggcactgac ttctctggat tccgtagcaa gaatcgggcg tatgtccttc caggagctgt
    14041 tcctgggaga caccacagat accgcaaaga tgactcccgt gcagagagtt ctgaccaata
    14101 agtattttgc aactgtgatc actttgtttt tcggttatct gctgactctt ggcggataca
    14161 acaatgtatg gccgctgttc ggttctgcga accagctgct ggcagcactg gtattgatcg
    14221 ccctggccgt attcttaaag actaccggtc gtaccggctg gacactgtac ttccctatgg
    14281 gaatgatgct ggtcgtaacc tttactgcat tgatccagaa gacagttgcc ctggtgaaaa
    14341 atgtggcatc cggacaggca accttcctgg tagacggctt acagctgatc gtagccatac
    14401 tgcttatggt actgggagtt atggttgctt acagctgcat ccgcaagctg ttcggtgccg
    14461 ggaaaaattc agaaaaagag gcagcataag ttgtcaaaaa aaatgatttt ccggtagaaa
    14521 agaaagataa gatatgctag aataaggaac ggtcaaggag taaaaaactc tttgaccgtt
    14581 cttttcgtca gagtggacgc tgatgggaag agtaataaca gagatacaaa tatgggaaga
    14641 agataaaagg ataggattga aaatgaaaca gaatatgcag gatctgacgg aaggaccctt
    14701 aggaaagaaa atactgatct tcagtgtgcc gttaatgctg tccaatctgc tacaggtgct
    14761 tttcaatatg gcggatattg cggtgatcgg tcagttcgcc ggttccctgt cgctgggagc
    14821 ggtagggtcc acagccacac tggtcaccat gtttaccgga ttcctcattg gtctgtccgg
    14881 cggtatcaat gtactgacgg cactgtatta cggggcaaag gatagggaaa gcctgtcgaa
    14941 gacgattcat tccgccgcac tggtcagcct gatcatggga gtgctgttac tggtactggg
    15001 agtcggctta tccgagtgga tgctgacaat gctgaagacc agagaggaac ttttggacaa
    15061 agcagtattg tacctgcgga tctattatct gggaatgccg gcgctggcca tttacaactt
    15121 tggtaatgcg gtgtacagcg cagtggggaa tacgaagaag cctctgtatt atcttggttt
    15181 ttccggagtg ctgaatatta ttctgaacct gttttttgtg atcgtctgtc atatggatgt
    15241 ggcaggagtg gcattggcca gtatcatttc ccagtatgta tctgcagcat taatcctgca
    15301 ggcactgctt cgctgcaaag acatttatgc cctgcatccg ggaaagctgc ggttggatcc
    15361 ggaacttacg aaggatatcc tgaagctggg aatgccctcc ggattgcaga acgtaatttt
    15421 ccagtttgcc aacagctttg tgcagatggg tgtgaattcc tttgatgcca ccatggtggc
    15481 aggcaattcc gcagccacca atgcagatgc catggtatac gatgtcatgg cagcattcta
    15541 taccgcctgc ggcagcttta tgggacagaa ttacggtgcg ggcaagaaga aaagggtgcg
    15601 gaacagttac ctcatcagcc tggcatattc cttcggcatc ggtctgatcc tgggagtgtc
    15661 actggtattt ttcggaagag cgtttttgtc cctgtttacc agagatgcgg cagttatgaa
    15721 tgccggtatg taccgtctga cgatcatggg attttcgtat tgtatttcgg catttatgga
    15781 ctgtaccatt gcggcggcca gagccatggg caagagcatt atacctatgt tcattgtcat
    15841 catgggttcc tgcgtgttcc gtgtaatctg ggtttacacc atatttgcct atttccatac
    15901 gataccgtcc ctgtatctgc tatatgtctt ttcatggagt atcaccgcag tggcggaaat
    15961 cctctatttc atacggatct acagacaaaa aatggctctc ctgtcgtaag acagaggagc
    16021 cattttgctg tgatgcgatg agccggccgg ctcgagggtg tatggattta tgcagcttcg
    16081 gtctctgcgg cagcagaaac agcagcgtaa cgaaccggca ggagatgagc gcgttccagt
    16141 gcaacataga tgatggggat cagaatcagc tggataatga taccggggag gcaggatacg
    16201 aaatatccgg cagcccaggc agaccagctg taggaaccgc ctgcaaagat cagtgcgcgc
    16261 aggataccgg tgatcacacg gccgcccagc atggcgatga ttaaagccgc atacagatca
    16321 atgaccagct taccgctgcg gagaaactgc aagaccagac cgctgatcag accatagagt
    16381 cccaactcga tcatcataac aggcatatat ccaaaaacgg gcataccggt aagcagactg
    16441 gataccagag gtccgagaat gccgcagacc agaccgaaag gtgctccaca gaccagaccg
    16501 cacagaagca cgggcagatg catgggggag aacaggacac cgcccttggg aatggcgtgc
    16561 agagtcatgg gcagaaccac acataaggca acacacagag cagtgaggat gctcttgttt
    16621 aaaacagtta attttttcat ggaaaacctc ctgataaatg gatagctgtt cagaaccttc
    16681 attcgcaaaa acgcctccaa gaggcttact ttttgcgaat tgaagtggct tcgccaaata
    16741 aatattgcca tatattctta cgaatgcgag cattcgacga acatctgtca atatttaatg
    16801 gttctgaaca gtaacaaaaa agccagaacg gcacactttt cccttctggg ataaatatgc
    16861 cttctggcgt taagctcctt cctgaagcat aatacataat aaagggtggg catttttttg
    16921 tcaagataaa ttgcaaaaaa gcatttgcca tgctatcatg gaacatgtat gtattatttt
    16981 tggcaggagt aaagttatgc gtattatggt aatggacggc cagggcggag gcgtaggcag
    17041 aagcctgctg gaggctctga aggaacggtt tccggaggcg gaactgatcg cagtgggaac
    17101 caacgccaca gctacggcga atatgatgaa gagcggcgtg acaagcggtg ccaccgggga
    17161 gaatgccgtg gtatataaca gtaagagagc agacgtgatc gtaggaccgg tgggaatcgt
    17221 catggcggat gccatgttag gtgagatcac ccccaccatg gcagcagcgg tagcttcgaa
    17281 tgatgcaaaa ctgatcctga tccccatgag taaatgccat gccactatcg tgggagtcga
    17341 gagcagacgc ctgggagaat acattgcgga ggctgtggat gagatcgctg agaactgttt
    17401 ctgagctgac agaggaattg cgtgtgcttc ccaccggaat gcgcattatt gccattgacg
    17461 gcagatgcgc cgccgggaag acaacactgg ctgccagact tgcaaaagaa ctgggcggag
    17521 atgtgatcca tatggatgat ttttttctgc cgcctgcact tcggacgcag gagcgcagaa
    17581 gcgagcccgg cggaaatgtg cactatgaac gatttttgac ggaagtcata ccgaagcttg
    17641 catcgggaca agcattttcc tatcagagat ttgactgttc ccgcatggca ccgggagact
    17701 ggctcccggt acaaaataac ggttttgtat tcgtggaagg cgcttacagc tgccatccgg
    17761 tactgggaga atacatggac cggaaagtat ttctggacat agaccatgag acgcagacgg
    17821 agcggatccg cacgaggaac ggagaggaca tactacagga cttcctgcag ctgtggattc
    17881 cgttggaaga agcatatttt caggcatttt ccctagagga gaataccgat tacattataa
    17941 aacagacgaa aggcgagaca gatgctgcat cagttttacc cggattatga ggcggattcc
    18001 gcctatggca tagattacag aacattatat gaacaggggt atcgcggaat catttttgat
    18061 attgacaata cactcgtacc gcacggggca ccggcaacac cgcaggcaac agagttcttc
    18121 ggcagattgc gggaactggg atttcagaca ctgctgttat cgaacaacaa ggagccccgc
    18181 gtaaaaagtt ttgcggatgc agtaggtgct tcctatatct ataaggccgg gaaacccgga
    18241 agagaaggtt atctgcgggc tatggaacag atgcataccg gggcggatac cacacttttt
    18301 gtgggggatc agctgttcac cgatgtgtgg ggagctaaaa aatgcgggat catgacttat
    18361 ctgacgaaac ccattcatcc gaaagaagag attcagatcg tactcaagag atatctggaa
    18421 aagatcgtac tgtttttcta ccatagagaa aagcgtcgga aaggaaatca aaatgctgaa
    18481 tgatatcaat ggatatacca gaacctgcgg actgatcgga aacccggtgg agcacaccct
    18541 gtctccggtg atccacaata ccctgtccat ggtgcttggg aaaaatctgg cctatgtccc
    18601 gttccatgtg gagaccggaa gactggagga tgccgtgaag ggtgcttttg cactgaatct
    18661 gctgggaata aatgtaacgg ttccctataa aagcgatgtg atcccgtacc tggatgacat
    18721 agatccgctg gcatcaaata tcggtgctgt aaatacgctg gtccgcacgg aaaagggcta
    18781 taaaggctac aataccgata tgccgggatt gtaccgggcc atgtgcgagg acggcgtcaa
    18841 agtcaaggga cagaaagtgc tgatcctggg agccggagga gtagccagag cggtagccat
    18901 gatgctgttg gataaggggg cgaaagaggc cattatcctg aaccgtacgg tggaaaaggc
    18961 acagagcata gcggacgagg tgaatcacct caccggaaaa aaacggaaat ttgcaaaagc
    19021 cctgtctata gatgcgtatg atacgctgcc ggaaggagaa cgctatctgg caatacaggc
    19081 caccagtgtg ggtatgtatc cggactgtga tgcggcggtg atcgagaatc ccacatttta
    19141 taagagagta cacaccggat atgatctgat cttcaatcca ccgaagaccc ggtttatgga
    19201 actggtggag gcacagggcg gtgaagccta taatggtgcg aagatgcttc tctatcaggg
    19261 aatcattgct tttgaattgt ggacaggctg tcaggtggaa ccctggcttg cagacaaagt
    19321 ctatgaacgg atgcagaaag caaaaaagac agaggacgga aaataaaaaa atgaaacaca
    19381 aacgaaggaa tatcgtcctg atcggcttta tgggatcggg gaagacaaca ctgggactga
    19441 aactgtccta tctgctacgg atgccggtag aagacacgga caagctgatc gagagacagg
    19501 agggcaggag catcacacag atttttgcgg atgacggtga atcctatttc cgggaactgg
    19561 agacggaact gctgcgaaaa tgcggggaac agaaatacga acgtatcctg tctgtgggag
    19621 gaggcactcc ggtcaatccg gtgaaccggc ccctgctgca tcagtgtgga acagtggtat
    19681 atctgcgagt gtcgccggaa gtggtctacg agcggctgaa aaatgacact acaaggcccc
    19741 tgctgcagtg tgaggatcct ctgacaagga tccgggaact gctggagatc cgggacaaaa
    19801 tctatgcgga atgtgcggac attatactgg atgtggataa caggcattcc gatgaactgg
    19861 cggaagagtt acagctgcag ttacggaagc agaaagacat acagagaaaa aaggagagaa
    19921 aaaagatgaa aattctggtg atcaacggac cgaatctgaa ttttctggga atccgtgaga
    19981 aaaagatcta cggaacacag gactatcagt atttactgga tctgatcgac aaaaaagcaa
    20041 aagaaaccgg agaggagatc caggtgttcc agagcaacca tgaaggagcc atcatcgaca
    20101 ggatccagga ggcttattct gacggaacag aggggatcgt gatcaacccc ggtgcctaca
    20161 cccattacag ctatgccatc cgggatgctc tggcaagcgt ggatattccc aaggtggaga
    20221 ttcatatttc cgatattacg agcagagaag agttccgcaa gatttctgtg acagcaccgg
    20281 tatgtaacag gcagatttac ggacagggac tggacggtta tctgcaggct atcgacttcc
    20341 tgcgggagaa caggcagtag tttcccgaga aatttgaaaa aatttttcaa aagtagttga
    20401 aatgtgcatg tttttatatt aaaatgttaa agaattatga cgtgaaaata tttaggagga
    20461 atacattatg atttctgcag gtgatttcag aaatggtatt accattgagt atgataacaa
    20521 tgtttaccag attatcgaat tccagcatgt taagccggga aagggcgcag ctttcgtaag
    20581 aaccaagctg aagaacatca agagtggcgg tgtagtagag aagactttcc gtcctactga
    20641 gaagtgccct caggcgcgta ttgatagaaa ggatatgcag tatctgtatg aagatggcga
    20701 tctgtactac ttcatggata ccgagactta cgatcaggtt gcactgaact ctgagactgt
    20761 aggcgatgca ctgaagttcg taaaagagaa cgagatgtgc aagatctgct cccacaatgg
    20821 cagcgtattc tccgtggagc ctcctctgtt tgtagaactg gagatcactg agaccgagcc
    20881 cggattcaag ggtgataccg ctaccggtgc cagcaagcct gctaccgttg agaccggagc
    20941 tactgttgca gtacctctgt tcgttaacca gggcgacaag atcaagatcg ataccagaac
    21001 cggtgaatat ctgtctcgtg cataattgtt gagactacca ctgttctgag caatatccga
    21061 taagagaacc tgtaaggcaa taagttatcc tgtttcggga tggcttattg cttttgttat
    21121 tttctacatt tttacaccgg aaataccaca tgggaaaaag caggagggga gcctgcttcg
    21181 gagaggcatg gaggtattct atggagattt ctacatttaa ggtaaaggta cagaaggctg
    21241 tcagcgaagt gttgggacag gagtatacag tggaactgcg ggaagtgcag aagaataacg
    21301 gagtgctgct tcagggactg atgatccgta agggacagga caatgtgaca cctacgattt
    21361 atcttaattc cttctgggaa gcttatgagg gtggcgtgac ctttgcagat attattaaga
    21421 agatcatcag tatctataag gaggacggaa tcggccggaa gatcgatgtt tccttttttc
    21481 aggattttga gaaggtaaag gagaaactgt gcttccggct ggtgaaccgg gagaagaacc
    21541 gggagcagct ggaaaaagtg ccgtatattc cgatacttga tctggcaatc tgcttctact
    21601 atgcgtttga cgaagcagga gtggacaacg gtatgatccc catctacaga tcccatctgg
    21661 acaactggaa ggtgacagac agggatctgc tggagattgc agtgaaaaat acccccagac
    21721 tgtttcccgg tgagaccatt cccatggagg atgtgctggg agacgcactg caggaacttc
    21781 cggaggaagt ccggagggaa tttttgagga aggtatccat gatgatcctg acgaattccc
    21841 gcaagactta cggtgcctgc agcatcctgt atcccgggat gctggagcag ctggcagagc
    21901 gtatcggcgg cgattattac atgatcccca gttctgttca tgaattcctg ctggttccca
    21961 gggaacggga acaggacagg gaagaactga agaaaatgat cgcagaagtg aaccggacgg
    22021 agctgccgcc ggaggaagtt ctgtcggacc atttatatct gtattgcagc cgggagaggg
    22081 aagtaaggat agtgtagaat accggagtga atattatgtt tgaaaaaaac tgaataattc
    22141 caacaattta aactggacat atgtagtgtt ttatgatatt atagtcgagg tgatgtaaca
    22201 aatttgtaat atttgcatcc gtagtctaat ggatagaacg gaggcctccg acgcctttaa
    22261 tgcaggttcg attcctgtcg gatgcattta catcaccttg attataggga gagagatgag
    22321 agaagccgct tgcggctttt tgcgattaaa gaactttttt tcagaatttg gaaaggacgc
    22381 aatatgtgga aagaaaaatt acattttctt ggaaactata tcataaagca tagtaaagta
    22441 gttttgccgg ctgtcgttgt tgtggcagtg gcagtcaccg tatctgtaag cctgagcatg
    22501 aacaaccgtc accgtgagga gcagcagaac gcagagagcg cagcggcagc gtccagtgag
    22561 attgctacgg aatcgacaac agaggatgta tctttggtag caaatgagga aggtgcggta
    22621 tatactctgg tagctactta ttataatgcc atggcgaccg gtgatgagac aacgttacgt
    22681 tccgtttgtg atgagatctc cgacaaagac atgtatcgtt atctggaact ggcacagtat
    22741 attgattatt atccgacttt ggagatctat accaagaccg gcccggaaga gggttcggtg
    22801 atcgcttatg tatattacaa gattgcattt gtgggtcatg aggaagaggt gcccggttat
    22861 caggcacttt atatctgcac caatgatcag ggagaaatgt atatcaagcg cggcgagaac
    22921 agtgaggaag tcaacgatta tatcaagacg gtaagcacgc aggatgatgt tgtggaattt
    22981 aacaacaaga tcacagtgga atacaatgaa ctgatggtag atcatccgga agtattgcag
    23041 tatattagtg agctggattc tcaggtaagc attgccgtag gagagaagtt ggccaatcag
    23101 gtagccggag atcagaatac agatacctct gcggaaggcg gtgatcaggc ggcagacggt
    23161 caggatactt ctgccgaagg cacagagcag cccgcagaag agcagggatc gcagtatgtg
    23221 acgacaacta ctaccgtaaa tgtcagaagc tccgacagtg agcaggcgga taagctgggt
    23281 aaagtggcag gaggtacgaa gcttcaggta ctggagcagc gcgccaacgg ttggaccaag
    23341 gtagattatg aaggtaagga aggctatatc aagacagagt tcctgcagct ggcagagagc
    23401 gcagccggtg ccgagaccat cggaaccgtg accgctacca ccaatatcaa tgtacgtgca
    23461 tccgccagtg agactgcaga tcgtctggga gttctttccg gtggcgattc cgcggaactg
    23521 gtcggcacag aaggcgactg gagcaagatc aggtacaatg gccagatcgg atatgtcaag
    23581 agtgaatatg tacaataatg agcagtgcca aaaaatgaag cggcaaaaat ctgactgctt
    23641 gcaggcagga tttttgatga ttcttttttg atagggcgaa tgcccgacag cgaagaaacg
    23701 cgaagcgttt ttgagctgta cactgcggaa tgaggaagaa gcaatttgcg gaagcaaatc
    23761 agcggaatcc gaatgtaggt agaattgcga gagcagcgaa ggctgcggag caattcgtat
    23821 tgcgcggcga atgatagggc gaatgcccga cagcgaannn nnnnnnnnnn nnnnnnnnnn
    23881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23941 nnnnnnnnnn nnnnnnnctg cggagcaatt cgtattgcgc ggcgaatgat agggcgaatg
    24001 cccgacagcg aagaaacgcg aagcgttttt gagcggtgca ctgcgggctt ggaataaaat
    24061 aagataattc agggaaagct tcttaccaaa aacggtaagg agctttttct atgaataaaa
    24121 caggaaatca caatctgaag aaacaggaga tcaacatcta ccgggagatc cagcgtaaca
    24181 cggaaatggc catgaaggca ctggatgccg ttacggacaa aatctacgac gacaggctgg
    24241 cagccgtggt ggcggaacag gaattacagt actccaggct acatgacagg gcagtgcaga
    24301 atctggtgga gggcaaggcc cagacctacc gggagaatgc catggagaaa atgatgctgc
    24361 gctttggaat cggttataac accctgctca acaccagcac aagtcatatc gcagaaatga
    24421 tcatcaaggg cagtaataac ggtgtgctgg agatggagaa ggtgttggca cacaatgaag
    24481 agtttgctgg aagcccctgc cgcagtctgg cgaaggaact gatcgagatg gagaatgcca
    24541 acatccggac tctgaaagaa tatctatagc atacaaaacg accgctttcg gcaatttaac
    24601 gtacatcata acgcacaaat tgtatacttg catacctttt tgtgcaattt ttatagaaaa
    24661 tggccaaaaa cttttacgat ttagtgaaat aaagtttatt gtacaattaa aaaagtcgtt
    24721 ttataataaa gcatatggca accaggttgt catgaatctg tataccaatt gaaggaggac
    24781 attcaaatgt catatgttga tgaagtattt gatttggtag tcgcaaagaa tcctgcacag
    24841 cccgagttcc accaggcagt taaagaggtt ctggaatctc ttcgcgtggt gatcgaggca
    24901 aacgaggaaa aatatcgtaa ggatgctctg ctggagagac tggtaactcc cgagagacag
    24961 atcctgtttc gtgtaccctg ggtggatgat aagggtcagg tacaggtaaa caacggtttc
    25021 cgtgtacagt tcaactccgc aatcggacct tacaagggag gcttacgttt acatccttcc
    25081 gttaacttag gtatcatcaa gttccttggt ttcgagcaga ccttcaagaa ctctctgacc
    25141 ggcttgccta tcggcggtgg taagggtggt tccgattttg atcccaaggg taaatccgac
    25201 agagagatca tggctttctg ccagagcttc atcacagaac tttacaaata tatcggagca
    25261 gataccgatg ttcctgcagg tgatatcggt accggcgcaa gagaaatcgg ttatatgtac
    25321 ggacagtaca agagactgac cggcctgtat gaaggtgttc tgaccggtaa gggcttaagc
    25381 tatggcggat ccctggcaag aaccgaagca accggttatg gcctgctgta tctgaccgag
    25441 gagatgttaa agtgcaatgg caaggatatc gcaggcaaga ccgtggctgt atccggtgcc
    25501 ggtaacgtag caatctacgc tatccagaaa gcagagcagt taggtgccaa ggtagttacc
    25561 tgttctgatt ccaccggctg gatttatgat cccgaaggaa tcgatgtagc actgttgcag
    25621 gaagtaaagg aagtaaagcg tgcaagactg accgagtatg ctgctgcaag acccagcgca
    25681 cagtatcatg agaagaagaa tggtgagcat ggtgtatgga ccgtgaagtg tgatgtagct
    25741 cttccttgcg caacccagaa cgaactggat ctggaggatg caaaacagct ggttgctaac
    25801 ggcgtattcg ctgtagcaga gggcgctaac atgcctacca ccatggaagc taccgagtac
    25861 ttccagaaga acggcgttct gttctgcccc ggcaaagcat ccaatgcagg tggtgttgct
    25921 acctccgctc tggagatgag ccagaacagt gagagacttt cctggacctt cgaagaggtt
    25981 gatgcaaagc tgaagaccat catggttaat atcttccaca atctggatga tgcagccaag
    26041 aagtacggca tggaaggtaa ctatgtagca ggtgctaaca tcgcaggctt cctgaaggtt
    26101 gcagacgcca tgactgcaca gggtattgta taggaacatc tgaaaacata aggtatatca
    26161 tgagcaccgg atcgaatgtg aaaacgtttg atccggtgat ttttttatgc aaaaaataac
    26221 ccgcccctgt agctgcgtgg agcagtggta caggggcggg tattgttatt tctatggtat
    26281 tctatggttc acatattatt cttctgtctg cggctgcgct gcagcattac gggcattgat
    26341 ctcggcctgt tgctggttga tcacagattc gtagtccgga ttggcaaaga aggttgcatc
    26401 atgctgacac tgacttctgg tgtttggtgt gctgacagtc tgcgtgccat ccggattatt
    26461 gatgatgaca gtggagccct gctgtaatgc aggatcttct accagaggca tggtggcaac
    26521 accggtgtac ttgaacgggc agtcggggct tgcgggtaat ccgtcgtaag cacagatctc
    26581 accctgatag tgcacatcac agctttcggt aggttctgta ccctccgcaa agtattccgt
    26641 gtagacggaa ccatcgcaga gaccgggaat cgggagcttg ccggactggg aacataccgc
    26701 tacctgtaca ataccggagg gaaccgggaa ctgggtgtta ggcagatcct catgaacacg
    26761 cttcataacc ttacgccata aggtcttgga gacaccgtcc tcggcactgt tcaggtcaac
    26821 attgttgtcg tacccggtcc aggtagcagc ggtgtagtaa ggggtatatc ctacaaacca
    26881 ggcatcggta ggtccggtag tagtaccggt cttaccggcg atagccatac cgccgaaatt
    26941 tacacggcca ccggtaccgg aggtaactac atcctgcata gcactggtca gcaggaaagc
    27001 ggtggtctcc ttgatgacct gtctggtagc cggattggtg ttgtccagaa ttacattgcc
    27061 atcggaatcg gtgaccctgg tgtacagctt cggggtgaca taagtaccgc cgttggcaat
    27121 agctgcgtag gcaccagtca gttcgtaagg agtaacaccg taggtcagac cgcccagagc
    27181 cagggactgg ttcacgtcgg acttgatctc gttgccgacc tgcacaccgt cggtcagagt
    27241 ggtaaatcca aagttcagca gataatcata accaagtctc ggagtgatca ccgtgatgtt
    27301 cttaactgcg atgatattca gggagtcacg gatcgcactt cggatattct ggatgccgcg
    27361 gtaaccagtc ttgtaccaat tccggacggg agtaccgtcg gcataattga acggagcgtc
    27421 gttgtaaaca gtagccagag tcttgccggc actgtccaga gcgggagcgt aggaggccac
    27481 taccttgaag gtggatcccg gcagacgctt ggcactggtg gcacggttca gggttctgcg
    27541 gccctccttg gcaccgcggc cgccgatcat ggctaccaca tagccagtgt tctgctcctc
    27601 aatgaccata gcggactggg gctgaggagt catggaaata gtttcttcgt aattatctgc
    27661 atcgtcttct acacccagct gagccagcat tgcggaacgg taagtgtcca ccgctgcata
    27721 ggcatcatcc tgggaagaga agatcaggtt gaattttgaa ttctggttct gcttgaacca
    27781 ggtcatcatg ttttctttac tgaagttatt cttggtaccg tcgggagaga taatggtaag
    27841 tgcatagttc agataccatt tcacgttctc cggataattg gagggatctg catattcttc
    27901 gttcagaatt gcctgaatct tgggatccag agtggattcc acacgcaatc cgccggaggt
    27961 cagcagagtc tcggccatgt tctcgttgta accggacagg atcagatcct gttttacctg
    28021 ctcatatacc gcatcagaga agtaggaacc ggtagtggcg ttggcttcct gatagtcaat
    28081 gtcgtacaga ccaatacgct cgtacacggc atcggtatcc gccatggctt catcgtattg
    28141 tgtctgactg ataaagccaa gctccagcat tttattcaga catttcttac gacgctttac
    28201 attctcttcc ggatgacgga tcggattgta tttggatgga ttttgggtga tgctggcaat
    28261 gaccgcacat tcggaaatgg tcagttcact gcagtgttta ccgaagtaac gtaaggatgc
    28321 ggattccaca cccagggtgt tctgccccag gttgatggcg ttcatgtaac gcagcaggat
    28381 ctcgtccttg gagtagtact ttgtaatctc cagtgccagg tactgctcct ggatcttacg
    28441 cttgattttt ttgatcttgt tattaccctc ggaggtccag tccgtaaaga cggtattttt
    28501 tagaagctgc tgggtaatgg tactggcacc ctgggcttct tcaccaccgg tcttaaaaaa
    28561 ctgataaccg gcacggataa tactcttgaa atcaataccg ttatgctgat agaaacgttc
    28621 atcctcgatg gctacgaaag ccttgcccag gtagtcggga atctcgtcca tgccgacgga
    28681 gagacggttg gagttggtgc tgacgtactg gtcaatctcg ttgccctctc tgtcatagac
    28741 gatggtagct tcgccgacag ccacgacatc cgacagacgg atcgtggggg tggaggagag
    28801 gataccgcgg aaaacaccga taccggcggc tacaccgcag atgccgatgc ctacgacagc
    28861 ggctagcatg accttgacac aggtcagagc aattttcctt ccccattttt gtgatttaga
    28921 gttgagtgtc ttttgcttcg cacggacacc ttttttacta taattcataa aacaagtcct
    28981 ttctgtctga gatacagacg aaaacattat accaaataat gctatgtaaa taaaggcttt
    29041 ttagaattgt ttcacatttt ggagaagtta tgctacactg aaaaagaaag caaaagaaaa
    29101 taagaattta aaaataagtg aggccataca tgggtgacat gacagcaaca gataactata
    29161 gagtcctttt gtccggtggg gaaggtgaga tcgtggagaa aaagtcccgt tttattgcta
    29221 cgatccgcaa gtgtgaaacc gaggaagagg cagtggcttt tatagaagaa atgaagaaaa
    29281 aatactggga tgccagacat aactgttcgg catttataat aggaagcaga ggcgaactga
    29341 cccggtgcag cgatgacggg gaaccctccg gaacggcggg gcgtccgatg ctggaagtat
    29401 tgaccggatc cggtatccgc aatattgcgg tggtagtgac cagatatttt ggtggcacgt
    29461 tactcggcac cggtggtctg gtgcgggcct atacccaggc ggtgaaggag ggactggacc
    29521 actgcgagac cggcattatg cggaagggct gcgaactgga agtgacaaca gattataatg
    29581 acgtgggcaa actgcagtac tatttcggac agcagggcat cgttcctgcg gacagcatct
    29641 atgcggaaaa cgtaaaattt attttacttg tcccggtgga aaaagaggat ggtctcagga
    29701 aaaacctgac agaaaccacc tgtggaaagg taaaaattga gaaattgaaa gaaacttatt
    29761 tcattgacaa agaatagtca taaagttaaa atattattaa caattcagaa ccattaaata
    29821 ttgacggata ttcgtcgaat gtttacattc gtaagaatat atgtcaannn nnnnnnnnnn
    29881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29941 nnnnnnnnnn nnnnnnnnnn nnnnnnngag caaaaggaaa gcctcgtaga ggcgattttg
    30001 cgaatggggt tctggatgga tcctacagaa aggtggtggt ttttatgaag tgtacacaga
    30061 agagttctga caacccactt ccggggcgaa gttactgcat aagttacacc catagaaaac
    30121 atcataagga ggatcatcat ggaacagatc aaagaactgg aagaagtctt agagcttctt
    30181 aacaccaagc agtataccaa actgcgccag tatctggctg aactcaacga tgccgatatt
    30241 gcaggacttc tggaagaact ggaagaagag gatatgctca aagtcttccg cattcttccc
    30301 aaggacctgg cggcagacgt tttttcctat ttagatatgg acaatcagca gaagatcatc
    30361 acctccctgt cagataagga agcaacgaac atcatcaaca acctgatggc agacgatgcg
    30421 gctgacctgt tggaagagat gcctgccaac attgtcaaaa agcttctgac caacgcaagc
    30481 cctgaggtac gcagagatat caatcatctg ctgcgatatc cggaagattc cgccggaagt
    30541 atcatgacgg tcgaatatgt agacctgaag gagaatctga cggtgaatca ggccatcgag
    30601 cggatccgta aggtaggcct cgacagtgag accatcaata tctgttatgt cttagatgcc
    30661 cagagaagac tggtgggaac cgtggccctg cgttatctgc tgctcatgga cggtgacgag
    30721 atcatcggtg acatcatgca tgaaaatgtc atctccatca ataccctgat ggaccaggag
    30781 gaagttgcca gacagttcaa aaaatacgac tttacagcca tgccggtcgt ggacaatgag
    30841 aaccggctgg taggtattat caccgtggac gatatcgtgg atatcatcga agaagagacc
    30901 acggaggata tggaaaaaat ggcggctatc gtgcccagtg ataagcctta tatgaagacc
    30961 ggggtatttg agaccttcaa aaagcgtatt ccctggctgc ttcttctgat ggtgtcagct
    31021 acatttaccg gagctatcat ttcttctttt gaagatgcgc tgagcgtgtg tgcggtactg
    31081 gtggcatata ttcccatgct gatggatacc ggcggaaatg ccggaagcca ggcgagcgtt
    31141 acggtcatcc gtggactgtc cctgaatgag atcgaatacc gggatgtgcc gaaggtcgta
    31201 ttcaaggaga tccgcgtggc actgatctgc ggtattacac tttccgcagc gaactttgta
    31261 aaattactgt tattggataa agtaggggtg ctggtggcag catccgtgtg cctgactctg
    31321 gtggcggcag tagtgatcgc tatgattatc ggatgtcttc tgccgatcct ggcgaaacgg
    31381 ctgggattcg accctgcggt catggcgagt ccgttcataa cgaccatcgt cgatgcaatg
    31441 tcattactgg tatatttccg tgtggcatct gcaattttag ggttatgata agagaataaa
    31501 tgataggtaa agcgtctgcc tgaaagcagg cgcttttgac atttttttga caggatgttt
    31561 ttttttaagt acttttcagc aagctctttg caaggatgta aatttttgtt agaatcaaga
    31621 taaatgtgaa atgtaaccgc tttcgttaat ctttttctta agagaatatt cgggaacagc
    31681 aaaagcaacg gaggaatgaa atatgagata tttttttgaa tctaaagtag agaaaaagga
    31741 tgtaggatac accattcaga ttccttttaa tgtatgggaa gtatgccatc agagagaagt
    31801 catcaaggga gatatcgttc tggataacaa tatcattcag tgcgagcttc atcccaagga
    31861 gaagggcaat tacgagattg tgatcaccga tgaagctgct gtaaaagtag aactgggtgt
    31921 gcctcacaag atcctgttac atattaacgg ttccctgatc cgtatggatc agaacagccc
    31981 ttacagcttt gagaatccaa tccgcaagat cgacagcatg aacgtgatca tccagcccga
    32041 agacggtctg tgcggtcagg cttgtgtcgc aatgttagcc ggtgttacta tcgcagaggt
    32101 gatcagcgtg atggactgca gagagtggca ggctaccatg ggccgtgtga tctctgcgct
    32161 gaactattac ggcattgacc ataccgacat catcgtatat accgagggta gacccgctgt
    32221 actgccgaag tgctgtatca tgatggaaaa gatgggacgt ttctgtcatt acctggtaca
    32281 ctatgatgga aaattctatg attccaatct gggtgtgctg gaagagtacg atatgagcaa
    32341 gctgttaggt taccttgaaa tcaaatgctg atggaatgca aatccgtatt tgcgtgattt
    32401 cgattacctt gttctattta taaggaagtt caaaaactgt aggttacaca gatgtgccta
    32461 cagttttttc atagaaaata ggttaatcgg ctaaaaaccc cctaataggg ttgcattttg
    32521 acacggtaaa gtgtaaaatg gaaaagtgtg gttttcgtaa cttttcagag cgattactaa
    32581 ttttctatta caaaggatgg gaagaaaaat gaatcagaaa aagaagattg aaaattatca
    32641 gcagattgcc atgggaaccg gattgcgtta tgatgaaacc aatgacttgt ttcatgggga
    32701 acgggatggg tttgacttta tcgtctatgc acccgatgca agatatccgt atatgatggt
    32761 actgcatacg gcggcaaaaa gcgctgacgg gtcgacattt gacaagcagg cagtgaaagg
    32821 gtttcagaaa agcagcaaga agatcgcgag ttttggtcag aagaatctgg atatcagagt
    32881 atcactgaag gcacagtcca atgcggaaaa atgtaaggat acattgaacg aagcacttgc
    32941 agctacaacg acattcctgc gtacaaacag ttacagcccc tgctgtgatc tgtgcggtca
    33001 gaatgtagag accggagcgt tcagaatggg cggtgaatac tatcatttat gtccggactg
    33061 cgagatgaag atgcgcagtg acatcgcgat gaatgcacaa cagacggcgc agaagaaaga
    33121 aaacatcgta ggcggtattg taggtgccct gttaggatcc ctgttgggaa tgctcagtgt
    33181 tctgatctta agccagttgg gatatgtagc agcattatcc ggtgtgatca tggcggtgtg
    33241 cgttctgaag ggatatgaga tgctgggcgg aaaactgacc aaaaaggcag tagtcatcag
    33301 cgctgtgatc atgatcctta tgacgtattt tgcagaccgt gtggactggg cgattatttt
    33361 gttcaatcag ggcggcggtg cggaagcagg ttatagtctg ttcgagtgtt atcgactgat
    33421 ccctgcggca ctttccgcag aaattattga tatgggaagc tacataggca atctggttct
    33481 acagtatctg tttgtggcgc tcggagcaat ccctaccgtt atcggaaaaa tgaaacagaa
    33541 aaaagaagaa ggtattatcg caagattaaa ttaagctctt tgctttcaat agacaatgtg
    33601 gtacaatagt accagtaatg taattcctca tatcgtggta ttgggttcag acttttgacc
    33661 ccggtagcat acctgttgga attaggggga aggaatatga gaaagtggaa agcatggctg
    33721 tttgcgctgg ctgttctcat aggcattggg actattggga cggtgtcagt aacggcagaa
    33781 gcacagaatc tgaatcaggg aaagcgggta ctgtttatca gctcgtattc ttatggatgg
    33841 gacacagtcc agacacagat tgaaggaatc aaagccggag tggacgagaa taccactatc
    33901 gactatgaat ttatggacac gaagcgtttt cggacagatg agtggctgaa tatgtttcat
    33961 gatatgctga aataccatct ggagaatacg gatccctatg atgtggtgat cgtgggagat
    34021 gatgcggcgc tgcagtttgc catggaatac cgggaagaac tgttcccgga gataccggtt
    34081 gtcttcgaag gtgtgaacga tgaagaatat gccatgaaag cggcagagaa tcctcttgtg
    34141 acgggaatta ttgaaaaact gtccgtagag aagaacatag acatggcact gaaggtgaat
    34201 ccgacagccg ataaagtagt ggcgatactg gatgattcag tgaccgcgga tgcagaacgg
    34261 aagaattttt acagtgctga ggcagaatat ccggagctgg aattttctga gatcaattct
    34321 tccgaactga caactgcgca gctgcagcag gctatcagca aggtggatga taagacgata
    34381 ctgatctata tcgttatgtc taaggacggc agtggcaaac aatataccag tgaccaggcg
    34441 ctgtgtatga ttgtggatta tgccaaagta ccggtttacc gcatggtgga ggccggaatc
    34501 ggtgacggac tgctgggcgg taatgtggta tccatgtata agtccggaga gatcgcagca
    34561 cagatggcca tggatatcgc aaatggtacc gacagtgcag agatcaatgt ggtaaaggac
    34621 agtcccaaca tttattgtgt agatgaagac gtcatgcgga agttcgggct ggaggcatca
    34681 cagtttccca aagataccga atttgtaaat catcgggaga atttttttgt caggaaccga
    34741 gaggctttga taccggcact tatacttatc actgcactca atgttattat ctgctgggtg
    34801 tgcttcgata attaccgcag acgaaagctg ctgcaggaac tggagcaggc acgtgcaatt
    34861 atggaggcgg cagcacagca tgacttcctg acgggacttc cgaaccgcag caaattcatg
    34921 aaggatctgg agcagatgat cgatgcgaag gttccctgta ccgtcatgat gctggatatt
    34981 gataatttca agaagatcaa cgacacttac ggacatacgg caggagacga agcgctgcag
    35041 caggtggcaa accgcctgaa agagatgcag tcccagatac tgacatcata ccgtttcgcc
    35101 ggagatgaat ttattctgat cctgcgcagc agccagaata tgctggtgga gaagacagcg
    35161 taccagtgca gacaggtatt cacaaaggat gtggtgctct gcggtacgaa gcggaagatc
    35221 ggcggcagca ttggtattgc aagttatcct aaggatacgg ataatctgga acagctgatc
    35281 gtgtgtgcgg atgatgccat gtatcaggtg aagaagaacg gcaagaatga ttttgcattc
    35341 tataaaaagc cagaggaaga aaaaacggac aatacgcaat aaaacggaaa tagaacaaag
    35401 aatacagaaa aatagggaaa caggcagaaa tttgctgtta taataacaga ggtttctgcc
    35461 tgctttttga acggaaaatt gctttccatg ttgaaaaaac atacgattat gttataatta
    35521 gtactattga ctaaaaggag aacagtgtga aaataaattt cacacgcaaa tttgtgcctg
    35581 cggcccaaac tttgcagatt ttgagaaagg catggttaat taaaatgcga atactatcag
    35641 aagcgctggg atatgtaatg tacttttgct attatctggt gcataattat ggattggcaa
    35701 tcattctgtt cacattgatc agcaagatcg tattgttgcc ggtatccgtc tgggtacaga
    35761 agaattccat taaaatggta aagatgcagc ctgagatcaa ccggatcaag gcaaaacatt
    35821 tcggggatgc agaccggatc gcggacgaac agtccaagat attcaagaga gagaagtaca
    35881 atccgctggc atccctgatc ccgctggcag ttcagattat tctgctgatg ggactggtag
    35941 aggttatcta tcacccgctg gattatctgc ttcatctgcc acaggatgtg atcacggcat
    36001 tcaatggcct ggctgtcagc ctggccggag caaatccgga gagctcctct attcagctgg
    36061 cagtagtgga gatgatcaag agtggtaatt atgcagaaca gttcgcagca ttgcagtcag
    36121 gactggcagg tgtggacatt gcgtctgtat tacagcaggt gcagggaatt tccctgaact
    36181 tctgcggcat gaatttaagt tgggtacctt ccgaagtggg gggcattgat atcatcgtcc
    36241 ccattgcagc cggtatttcc gcatggctgt tgtgtgtggc acagaatgca gccaatgtca
    36301 ttcaggcaga gcagtccaag ctgaacaaat acggtatgat ggcattttcg gtaggcctgt
    36361 ccctgtatct gggctggttt gttccggcag gtgtggctct gtactggatc gccagcaacc
    36421 tgttcgctat tttacagcag tatctgctga actgggccat caatcccaag gattatgtgg
    36481 attatgagga actggaggcc agcaaacagg aactggagga attgcagtcc attggtggca
    36541 agaagaaact cttcgagaaa aatccctatg ccaagagaga gaagaaagat ttcaaacgtt
    36601 tcttctctgt agtgaacaag catctggtat tttattccga gagcagtggc ttctataaat
    36661 attatcaggg cattatcgaa tggctcctgg cacataccaa tctgactatc cactatatca
    36721 ccagtgatcc ggaagatcag atctttgcac tggcggaaaa agaagacaag atccgggcat
    36781 attatatcgg agaaaaacgt ctgatcacac tgatgatgaa gatggatgca gacgtagtgg
    36841 tcatgactat gccggatatt gagaacttcc atattaagcg cagctatata aggaaggata
    36901 tggaatatat ctatattcct cactgtatgg acagtctgaa tatgaccatg cgtaccggtt
    36961 ccatggatca ctacgatacc gtatactgcg tcggtaagca tcatactgag gaaatccgca
    37021 agacagagga agcttacggt ctgcctccca agaagctgat cgactggggc tactgcctgc
    37081 tggatcgtat gatcgaagac tacaggaaag cggataagac gcctcatgag aaaaagcata
    37141 ttctgatcgc tccttcctgg cagaaggaca atatcgtgga cagctgtctg gaaggcatgc
    37201 tggatgatct ggcaggtaag ggctatgaag tggtagtacg tcctcatcct caacaggtgc
    37261 gtctgcagca ggataagatg gatcgcctga aagagcgtta tgccaagaac ccggatattg
    37321 acattcagac agacttctca tccaacagca cggtattcga ggcggatctg ctggtgactg
    37381 actggtccgg tatcgcttat gaatatgcct ttacgaccaa gaagccggtt ctgttcgtgg
    37441 atactcccat gaaggtcatg aatccggaat accagaagat cgacaccgta ccgatcaata
    37501 tctggatgag ggatgtcatc ggagaccgtc tggatcctac aaagacagag gagaccgaaa
    37561 gcaaggtcag aaatctgctg gagcagaagg atgcttacca tgacagaatt gaaaagttcg
    37621 tggaagaata tgtatacaat ctgggcaaca gtgccgaagt aggtgcaaag tacattgtac
    37681 aggcagtcca ggaacagatt aaaaaagcaa aagaacaata aaaattgaaa tacaaataga
    37741 gtaaaagaaa gaggtaaaaa catgaaaaaa ttaagcaaga tcgcacaggt aacatttttt
    37801 gcagcagcat tcgcagtact gttttccgga acagcagatg catatattga tccttccgtt
    37861 atgaactatg ctattcaggc agtagccggt gtagtcatcg ctgtaggcgc tgcagccgga
    37921 atctactgga gaaaagccaa gaagaagatc gacaagagcc tgggaatcga cgagaacaga
    37981 aacaaagaag tagaatccga tgatattatg ctggatcagg cagaggacaa gcaggagaag
    38041 taatctgaca tgaaaaagcg ggataaaaag caactgctgc cgggaatcgt ggtggcggct
    38101 gccatcagtt tcctggtgtt tttatatgca ccggtagact tgtattgcag taatatatcc
    38161 gagttctggt ttgatttcgg aattctgatc agggcatcat tgggcctgtt tttgatcagc
    38221 accgttgtac tgcttctgat ctatattgtc ttgtggctga tccatccggt gctgtaccgt
    38281 ctcggactgg caggcggatt cttagggctg atctgtacct atattcaggg aaattttctg
    38341 atcaggaatc tgccgcccct ggatggaacg acgattcgat gggaagaata tacatttctg
    38401 aggacagagg attttattct ctggggcatc ggctttgttg ttgtagtgct catgtatatt
    38461 ttcctgaaaa aagatctttt gtccaaaacg gtaatgtgtt taagcggtgg attgacattg
    38521 atgctgctga tcacagcagt cagtattgta tttaccagtg gtgcactgca ggaaaaagta
    38581 cattatcaat atggtgcgga cgaacagttt gtgatgtccg aagaccagaa cttcgtgatc
    38641 cttctgctgg atacggtgga ttccagaacg atcacggagc tgttggacga gcatccggaa
    38701 tacagtgagg tatttcgtga tttcacctat tttgaaaata cggtaggagc atactcctgc
    38761 acagagagat ccgttcccta tattctgtcc ggagactggt atgaaaatga tgaacccttt
    38821 gatgattacg gcaagagggt gtaccaggag tccccactgt ttcagacatt gcaggaacgt
    38881 ggctacaata tggaactctg tgattctgaa ttgtatatgg ataccgaact gatgcatatg
    38941 ttcagcaaca ttcacgtagt ggatttcgaa ctgagttctt atacgaaatt tgcgaaacca
    39001 ctgttgaagc tgatcggttt ccggtatgcg ccctttgaac tgaagaaaat gtgtacattt
    39061 aaaaaggctg cattcgcaga actgctccag gtagagaata cacctgagga aaccagctgc
    39121 agtgaaacgg atcatgtatt taagagccag ctggatctgc ggggaattac cgtggaagat
    39181 tcctccaaaa aattcaaatt tattcatctg aacggagcac atgttcctta tatctatgat
    39241 aaggacatga atatcatcaa tgaactggac ggcacctatg aacagagcgc acaggccacg
    39301 atggtcggtg caatggatta tgtcgaacat ctgcgcaaca gtgaagccta cgacaataca
    39361 gtcctcattg tgatgtcgga tcatggctat aacggaagcc tgggacaaaa tggagaggct
    39421 acctggatgc gtcagtgtgc attacttctg atcaagggaa gaaatgagca tcatgatacc
    39481 atgcagatct cgcaggcacc cataagcttt gaggatctcc aggaggcata tgtgcgtctg
    39541 ctggacggca ggcggagtga tgaggtcttt gactggaagg aaggcgatgc aagagaacgc
    39601 cggttcttac ggtattcttt cctggatgac gatcatatgc aggagtatat gcagacgggg
    39661 tatgcttctg atatggatac catgattcct accggaagag agtataatcg ttagaaagaa
    39721 atgcaacaat gtgacaggga gaataagtgt gagtaaaaat actgtgagaa aaaagataac
    39781 aggcagacag ctgatgccgg ggattttcat atccctggct atcagctttt tgttgttttt
    39841 atatgcacct gtcgatctgt actgcgcgaa tgtttcggaa ttctggtttg atttttccac
    39901 gttattgatc actgcactgg ggatgtttgc agcctgcttc gccgtactgg cggtgttgta
    39961 tctgattgcc atgctgattc atccgtatgt gtaccggatc gcactggctg gagggctgac
    40021 actttttatc tgtacctata tccagggcaa ttttatgatc gacaagctgc ctccgctgga
    40081 tggaacgacc atcaaatggg agaattatac gatactgcgg acagaagatc tgatcctctg
    40141 gagtgttgct ttgctgatcg tcgttcttat gtacgttttt ctgaaaaaag atctgttttc
    40201 cagaaccgta atgtatctga gcggtggact taccctgatg ctgttgatca cggcagtcag
    40261 cgtagtattt accagcgggg cattgcagga gagagcgcgt tatcagcttg gcggagataa
    40321 cgaattcgta atgtctgatg aacagaattt tataattctg gtattggata ccgtggactc
    40381 cagaactttt gcagaattgc tggagacaca tccggaatat gcagctgagt ttcaggattt
    40441 tacttatttt gaaaataccg taggggcgta ttcctgtacc gagagagccg taccctatat
    40501 tttgtcaggg gaatggtacg agaatgatga accgtttgaa gattatatgc gccggatgta
    40561 ccgagaatct ccactgttcc ggacattaca ggaacgggga taccggatgg agttttatga
    40621 tgaagaactt tatctggatg acgagatagc ccagatgttc agcaatgtat accgggtgga
    40681 ttttgaactg agttcctatg tcagatttgc aaaacctttg ttgaaactgg tgggattccg
    40741 atatgcccct tttgaactga agaagaaatg tattttcaag atggcggcta tcgatgaact
    40801 ggtcaaagtg gaaaatgcac aagaaaaata cagtttcagc cagcaagatc atatatttaa
    40861 gagtcaactg gaccaaagag ggatcagtga acaggataca caggcaaaat tccagtttat
    40921 tcatttgaat ggagcccatg tccccttcat ctatgatgaa aatatgaata ttattgatga
    40981 agaggaagga acttatgaac agacgatgct ggcggtgctc tccggggctg ggaactatgt
    41041 ggagaagctg cgcggaagtg gagcttatga caatacggtg ctgatcatta tggcggatca
    41101 cggatacaac ggaagcctag atgagagcgg agaggaagcc tggatgcgtc agtgtccgct
    41161 gctgttgata aaaggcatgg gggaaaccca tgataccatg cagatctcac aggcacccat
    41221 aagctttgaa gatctgcagg aagcatatac acgtctgttg gacggaaagc agagcacggc
    41281 cgccttcgac tggaaggaag gcgatgtaag ggagcgccgg ttcttaaaat attcttttct
    41341 ggatgaggat cacatgcagg aatatgtgca gacgggatat gcgtttgaca ggaataccat
    41401 gaaggctacc ggaagagagt ataatcgtta gaaagaaatg caacaatgtg acagggagaa
    41461 taagtgtgag taaaaatact gtgagaaaaa agataacagg cagacagctg atgccgggga
    41521 ttttcatatc cctggctatc agctttttgt tgtttttata tgcacctgtc gatctgtact
    41581 gcgcgaatgt ttcggaattc tggtttgatt tttccacgtt attgatcact gcactgggga
    41641 tgtttgcagc ctgcttcgcc gtactggcgg tgttgtatct gattgccatg ctgattcatc
    41701 cgtatgtgta ccggatcgca ctggctggag ggctgacact ttttatctgt acctatatcc
    41761 agggcaattt tatgatcgac agactgccgc cattggacgg aacttcaatc tggtggggca
    41821 aatacgatat tctccggaaa gatacattgc ttctttgggt agtagtgctg atcgttgtaa
    41881 ttgcggcaat gatcgtgctg cgaaagcaga aatttgtcca tgtggtaatg ttcatcagcg
    41941 gatgcatgac cctgatgctt ctggttaccg catgtagcac cgtgattacc agcggggcac
    42001 tgcattccaa actgcatctg catgtcagcg tggaagaaga attcgagatg tctgcggaca
    42061 acaattttgt gatccttgtg ttggatacgg cagattccag agagtttacc tcactgttag
    42121 aggaacatcc ggagtaccgg gagatctttg gagattttac ttattttgag aataccacgg
    42181 gagcttacac cagcacattg aatgctattc cttttatttt atccggtgaa tggtatgaga
    42241 atgagggatc ttttcaggac tatctggaca gagtctacag agaggcaccg ttgtggaagg
    42301 aactgaagag cagaaattat cagatcgatc tctatgagga tgatatccgt gcacaggatg
    42361 cttccatcgt ggacaatttt gacaatgtct attttactaa ggtcaccccc gtttcttatc
    42421 tggaactggc aaagcaggag ctgaagctcg ttggattccg gtatgcaccg tatgatctga
    42481 aaagatactg cgtgatcaaa gaagtctatt ttaatgaatt acgggaatct catccggaag
    42541 gttcggattc caaggcgttt actgcggata atacggcttt caaggaggat ctggcagccc
    42601 agggtgt
//
LOCUS       DAWEJW010000009        40779 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_443_length_40779_cov_59.073102, whole genome shotgun sequence.
ACCESSION   DAWEJW010000009 DAWEJW010000000
VERSION     DAWEJW010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 40779)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 40779)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..40779
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_443_length_40779_cov_59.073102"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1521..1620
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    31059..31158
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        11490 a         8333 c        11037 g         9719 t
ORIGIN      
        1 aagctgtcca tcaccagtga ggaatccaag gccatcaatt ttgacgagac ccccaaggtc
       61 atcatttccg cttccggtat gtgtgatgcg ggacgtatcc gccatcatct gaagcataac
      121 ctgtggagag aagaatgtac gattctcttc gtgggttacc agtccgtggg aaccctgggc
      181 agagcgctgg tagagggtgc cgatgaggta aagcttttcg gtgagaccat tcaggtgcgg
      241 gcacagatcc ggaaactggc gggattaagc ggccatgcgg acaaagaagg actgatcgag
      301 tggatccagg cctttgaaga gaagccgaaa aaggtgtttg tggtacacgg tgaagacagt
      361 gcctgcactg cattcaccga atgcctgaag atcgagtatg ggcagagggc ttacgcacct
      421 tatagtggca cggagttcga cctgatcagc aataaattta cctatgaggc tgcaccggtt
      481 gccgtgaaga aaaagacgaa gcctgtcacc ggagtcttcg agaggctgtt ggcttctgca
      541 agacgtctgt tggcgctggt taccaagagc gagggtgtca tgaccaataa ggacatggcc
      601 aagttcgccg accagatcaa ttccctgtgt gacaaatggg aaatataagt gcattcatag
      661 gaagtatttc tgactgcttg caggcaggaa atacttccta tgagaggcat aagcaaccct
      721 gagtatgcag gacgagagtc cggaatacga aggggtgtac gatttcgaga gtgcgaagcg
      781 cggagaaatc ggttatataa cataataaga ttctccatca gcttctgcgt gactgcttgc
      841 agtcaaggct tttggctgat gagagaatca aactgatatg agtaagcaag gcggtagcct
      901 ggaatacgaa tatcagagcg atttcgagag tgcgaagcac ggagaaatcg gttatatatt
      961 agaagataga ataaggagac aacatgagct tagctgatca tatttttatc gatatgtgta
     1021 atgacattct gaccaacggt accagcacgg agggcgagaa agtgcgtccc cattggccgg
     1081 acggcacccc tgcctacact attaagaaat tcggagtggt gaaccggtat gacctgtcga
     1141 aagagttccc aatcctcacc ttacgaaaga cggcgctgaa atccgccacg gatgagatgt
     1201 tgtggatctg gcagcgcaag tccaacaaca ttcatgactt aaaaagccat gtctgggatg
     1261 aatgggcgga tgcggatggt tccatcggta aggcctacgg ttaccagatg ggtgtgaaac
     1321 atcagtataa agagggcatg atggatcagg tggatcgtgt gatctatgat ctgaaaaaca
     1381 atcctttcag ccgccggatc atgaccaata tttacgtgca tcaggacttg catgagatga
     1441 atctgtatcc ctgtgcctac agcatgacct ttaatgtgac tcagaaaaag ggagaggaca
     1501 ggctgaccct gaatgccatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 ggggagcaga tcagggatat tccgattgca atctgacatg agtaagtagg gcggtagccc
     1681 ggaatacgaa tatcagtacg gtttcgtgag tgcgaagaac ggagaaaccg gttattatca
     1741 tagaaacaaa cagaaacgag gtacaccatg aatttaattg tagcagtaga caacaactgg
     1801 gccatcggca gcaagaacga actgctgatc cgcatcccca acgatcacaa gcatttccgc
     1861 gaagagacga caggtaaggt agtggttctg ggacgcaaga ctttggagac tttcccgcag
     1921 ggcatgcctt taaagaaccg catcaacatt attttgtcca aagatccgaa ttatcaggtc
     1981 aaggatgcga tcgtggtaca ctctattgaa gaattgctgg aagaactgaa gaaatatgac
     2041 gaagagaata tctatatcat cggcggagag agtatttacc gccagatgct gccttactgt
     2101 gacgtggccc atgtgacgaa gatcgatcat gcctacgagg cggatgccta tttcccggat
     2161 ctggacagtg atcccgagtg ggagatcacc gcggacagcg atgagcaggt ctattttgac
     2221 attacctatc agttcttaaa gtacgagcgc aagaaagcct gagtgcggaa aacgatgaaa
     2281 aagaagatat atttggcagt tacggtttta ctgcttactc tggcaggaat atatgtggca
     2341 gccagaatga ttctgtgggg tggtcccgta agttttcgca gttatgtgtt gttactgggg
     2401 gatctgctgt gtctgctgct tctcctgttt tatgtatatc tgagagtacc catcaggctg
     2461 caccggaaaa tacgggacaa gtggggaact cccgggaaat gggagtggct ggaggatatc
     2521 ggcattgctt tgtgggcagt gctgtgcctg atcgtcacgt ttgccggaat tgcctggatt
     2581 tggtttgaaa atgacatccc ttcggagacg cggctcacag agaatctgat cagtgtggaa
     2641 gagatggagt tcccggacag gcactggtat tcctactggc gtccagaggg cgtgctgttt
     2701 cggagagaga cggagcttgc cggtgaggat tacattactt atctgacgga attgatggac
     2761 aaggaagaaa atacccaccc ggaggatgca gaaacggtag caaagcaggc gatggaagaa
     2821 cagtggcttg cggaatacca tgcgtcggag acggaaaccg cggaagatgt acaggatccc
     2881 ttagagcctt acgcacaggc agtatatgat gctgtgctgc aacaacaggg cttttccttc
     2941 gaagtctgct acaatgccaa aggcaatttc tatctgcagc tgggagaaca ggagcaggcg
     3001 ggaaagacct atgcatacac gctggtctac aatcgtacct cgcagaacgg aaaatgtgag
     3061 attttcgtgc tgtaccggaa tccggaagag aatctgtcca cagaggcagc acagatcgtg
     3121 gagttttatg cggtccgcag tgaagatctg caggtcattg cgggggataa gaccggatgg
     3181 tcagtggtag cgaacagtgc atatcaggaa gctaccggag aaaaataatt gtagtgaatt
     3241 taatttgagt tttcagttaa aaggagaaat gagccacatt ttcgctgaaa aaagcactgg
     3301 aaagcataaa tgtgtatatt gacttttatt acaaatggta taataatgaa aatcaataaa
     3361 ttctgttaag agaaatcaac aggcagagtg gtaaaccgag gaaagaagga atacaaaatg
     3421 gaaaagaaga acattgactg gggtaacatc ggtttcggtt atatccctac ggattacaga
     3481 tatgtgtcca attacaagga cggcaaatgg gatgacggtc agctgaccca ggatcccaac
     3541 atcgtattaa atgagtgtgc aggtgtattg cagtatgcac agaccgtatt cgagggtctg
     3601 aaggcctaca ccacagaaga tggacatatc gtaaccttcc gtcctgactt aaacggtgaa
     3661 cgtctggagt cttccgcagc aagactggag atgcccattt tccccaagga gcgtttcgtg
     3721 gaagctgtta ccgagacggt taaggcgaac gatgctttcg tgcctcctta cggcagcggt
     3781 gcaactctgt acattcgtcc ttatatgttc ggcagcaatc ctgtcatcgg tgtaaagcct
     3841 gcggacgaat atcagttccg tatcctgtgc actcctgtag gaccttactt caagggcggc
     3901 gttaagcctc tgaccatccg tgtcaccgac tttgaccgtg cggcacctca cggaaccggt
     3961 cacatcaagg ctggcctgaa ttatgctatg agcttacatg caatcgtatc cgctcacaag
     4021 gaaggctacg atgagaatat gtatctggat gctgctaccc gtaccaaggt agaggagacc
     4081 ggcggtgcga acttcctgtt cgttaccaag gacaacaagg tcgtaactcc caagtccgac
     4141 agtatccttc cgtccatcac ccgtcgttct ctggtatatg ttgccaagga atacctggga
     4201 ctggaagtag aagagagaga agtatacctg gatgaagtaa aagacttcgc agaggctggt
     4261 ctgtgcggta ccgcagcagt aatctcccct gttggcaaga tcgttgacca cggcaaagaa
     4321 atctgcctgc ccagtggcat gactgagatg ggacctgtca ccaagaagct gtatgagact
     4381 ctgaccggta tccagatggg caggatcgaa gctcctaagg gctggatcca tgtaatcgag
     4441 taatctggta tcaaaattga ataatattaa aatgcgtgtg gagaatatcc gcacgcattt
     4501 ttttgtctgt agaattctta gatttttctg aacttccaag aaaccgtggc acagaagaaa
     4561 aaaatagggt ataatcagaa tttagagact ttgagaaagt aggaaatgtt atgtttttta
     4621 aaaatagatg tcatatcacc gcggtattgg tggcgggagt gctgggaata agtatggtga
     4681 cgggactgac cgcctgcggc agcagtgacg gcacaaaagt ggtatttacc acaggatttg
     4741 ggaaaaatga ggtatttcgg atcggggacg agtcctgctc taaggcggag atcatgatct
     4801 acctgaccac gacccagaat caatatgaga atgtctacgg gacggagatc tggaatactt
     4861 ccctgaacgg agtgacgctg gaggataatg tcaaggagac agtgctggca agaattgctc
     4921 agatcaagac catgtacctg ctggcaaaag agaaagaggt cacgttggat gaagccgagg
     4981 aggcaaaggt cgtccaggcg gcacaggagt attattcttc cttaaatgat acggagatcg
     5041 aggctatggg tgccacggag gagattgtgg agaatctgta tcgggaatat gccatggcag
     5101 acaaggtcta tcagttgatt atacaggaca tcaatccgga gatcagtgat gacgaggcac
     5161 gcaaaattac ggtgcagcag atcttttttg caacagcatc cacggatatg gacggaaacc
     5221 tgaagcctta ttcggaaagc tccattcaga gtgcctatga gaaagcctgc gaggtaagag
     5281 acctggcggt ggacggagaa cacgatttca cggaactggc atccaaatac agcgacgatt
     5341 ccgagatcac ctattccttt ggcaaaggag aagcggagag cgcttatgag gaagcggctt
     5401 tcaatttggc aacagacgag gtgagccagg ttgtgaaatg tgaacgaggc tattacatta
     5461 tcaaatgcat caatacgctg gatcgggaag agacggacgc taataaatta aagattgtag
     5521 aagagcggaa acgggaagtc ttcgggcagg agtatgacag ctttgtgaat acactggtgc
     5581 gtcagctcaa tgacaagctg tgggacgaga tcacgctgat catggacgaa caggtgacta
     5641 cggacaactt ttttgatgtg tatgcaaagt actttccgga ggagtaattt ctggtttttt
     5701 acacgcttta ttaaaatttt agaattacgg aaatattgat aaatactgga tttttgactt
     5761 attgttagca ctcatttata atgagtgcta aatttgtctt gacttttcaa ttttacagtc
     5821 ctacacttag aatcagttag gaaacagtca gccatggctg tgcaatttta gaaaaggatg
     5881 tggtaataat ggcagcaaaa aacggtagtt tgtcaattaa cagtgaaaac atatttccga
     5941 tcattaaaaa gtggttgtat tccgatcacg atattttcta cagagagttg atctctaacg
     6001 gctgcgatgc cgtgaccaag ctgaaaaagc tggacatgat gggagaatat gccctgcctg
     6061 aaggctataa gggacgtgtg gatgtcatcg tggatcctga aaagaagaca ctgaccttca
     6121 aggataacgg tctgggcatg actgacgacg aagtggaaga gtacatcaac cagatcgcat
     6181 tttccggcgc aacggatttc attgagaaat ataaggacaa gagcaattcc gaccagatca
     6241 tcggtcattt tggactgggc ttctattctg cattcatggt agcggatgaa gtacacattg
     6301 ataccttgtc ctacaaggac ggcgccaagg cagtgcattg ggagtgcgac ggcggcaccg
     6361 agttctccat ggaagacgga gacaagacgg atgtcggtac gaccattacc ctgtttttaa
     6421 atgaagattg tctggagttc tgtaatgagt acaaggctcg tgaggtcgtg aagaagtatt
     6481 gttccttcat gcctaccgag atctatctgt ccaaggcgaa caccaaggag acccagatca
     6541 tcaaggaaga ggaaaagctt cctgatgatg aagtcctgga agagatcgag ccggagaaga
     6601 aggaagcggc agaaggtgaa aacgctgaag ctgctgaggg aacagaagag cctaagaagc
     6661 tgaagatcag aaagcgtcct gaattgatca acgagacaca gcctctgtgg accaagcatc
     6721 ccaatgagtg cacgaaggaa gagtatctgg acttctaccg caaggtattt caggattaca
     6781 aggagcctct gttctggatc cacctgaata tggattatcc ttttaatctg aaaggtatcc
     6841 tgtacttccc gaagatcaac atggagtatg agtccatcga aggtaccatc aagctttaca
     6901 acaatcaggt atttatcgca gataacatca aggaagtcat tcctgagttc ctgctgctgt
     6961 taaagggtgt catcgactgc ccggatctgc ctctgaacgt atccagaagt gcattgcaga
     7021 atgatggatt tgtcaagaag atcgccgagt acattaccaa gaaggtagcg gacaagctgg
     7081 ccggtatgtg caagacggat aaggaagagt atgacaaata ctgggatgac atcagtccct
     7141 tcatcaagtt cggctgcctg aaggatgaca agttctgtga gaagatgaat gattatatcc
     7201 tcttcaagaa tctggacggc aaatatctga ctctgcccga gtgtctggag atcaagcctc
     7261 aggacgagga agagaataag gaaaatgctg tagatgagaa cggcaacaag gtagaagctg
     7321 aggtcgtagg ggataaatct gaagatgagg cttctgagga gaatgctgag gaagagaaga
     7381 aggaaaagac catctactat gtaacggatg aacagcagca gagccagtac atcaatatgt
     7441 tcaaggctgc caagatggat gctgtgatcc tgacccacaa tatcgatcag ccctttatct
     7501 cccagttaga gcagaagaat gagggaatca agttccaacg tatcgatgcg gatgtgaccg
     7561 atgccctgaa gtccaagact tccaagaagg ctgaaaaaga gatggaagag caggcagaga
     7621 gcatcagcaa gctgatgaag aaagctctga agaacgacaa gatcactgtc aaggtagaga
     7681 agctgaagaa caagaagatc tcttccatga tcacgctgtc cgaagagagc agacgtatgc
     7741 aggatatgat gaagatgtac tccatgcccg gcatggatat gagtgctttc ggcggtgagg
     7801 gcgagaccct gattctgaat gccaaccatc ctctggtaca gtacatcacc gagcaccagg
     7861 acggcgagaa cgcagaaatg atctgcgagc agctgtacga tctggcaaag ctccagcatg
     7921 cacccttgtc cgccgatgcc atgaccaagt tcgtggcaag aagcaatgac attatgatgc
     7981 tgttgaccaa ataaaaatga cggattaaaa acaaagggaa tcacaatctg ggaacggttt
     8041 gtggttcctt ttttgggcta tacggcggct cggggacaga tgcagaccgg taccggaatt
     8101 agtatttagt gaatgcatat cacccgtctg ttaatcaagt agatgtatga tgaagacaaa
     8161 acggctagaa gttagtctac ttagtatatg ggaacatgca catatttttc tgactttgca
     8221 tattctgtaa ggaacatttg ttgcctgacc ggatcaagac agacaaaacg gcataggcag
     8281 aaaggacctc tgtccatgga cactacttat acaggaacac ttcagatcag cgttgtctcc
     8341 gctctgggga tgacaccgat acccggcgcg accgtcactg tctcctatac cggggatccg
     8401 gattccccgc tggagacgat gactactgac gaatccgggc aaacccctac gataacactg
     8461 gatgcgccgc cgagagagct ttctttatct ccggatatta cggcacagcc ctattcggag
     8521 tacaacattc aggtgactgc agaaggcttt gaaccggtac tggtatctgg ttccgaaatt
     8581 ctggcaggcg aattttccct acaaccgatc cgcatgaatc cgctgaatgt aacagaggag
     8641 gaagaaaaag tagtagtaat acctgcacat acgcttttcg gagagtatcc tccgaagatt
     8701 ccggaagaag aaatcaaacc tatgaacgag accggggaga tcgtactgag ccgtgtggtg
     8761 attccggaat atgtgatcgt acatgacggt gtcccggagg atcccactgc ccggaattac
     8821 tgggtgcgct ataaggatta cattaaaaat gtggcctcca gcgagattta ttccacctgg
     8881 ccggagagtg caatttatgc taatattctg gtcattcaat catttacgtt aaaccgtata
     8941 tatacggagt ggtacagggg acgtggatac gattttacga taacttcttc tacggcgtat
     9001 gatcaaaaat ggatttatgg cagaaatgtg ttcgaagaga tcgactatct ggtagattcc
     9061 atttttacca actatttgtc aaggcccggt gtacgacagc ccattttcac atcgtattgt
     9121 gacggtaatc ggactacctg ccgggggctg agccagtggg gctccaagtc attggcggat
     9181 cagggatatt ccgctattga tatcattcat tattattacg gcaatgatat gtatatcaat
     9241 tccgcagata ttatttcggg agtgccttct tcctggccgg ggtatgatct gactatcggt
     9301 tcttccgggg aaaaagtccg tcagctgcaa cagcagttga accggatcgc cagaaattat
     9361 ccggcgattc ctacgttgac acccgacgga atctatggct ccggtactgc ggaggctgtc
     9421 cggacgttcc agaagatatt taatcttcct cagaccggaa tcgtggatta tcctacatgg
     9481 tttgaaatct cggatattta tgtgggggtg acacgtattt cggagccgga tgcgtagtaa
     9541 atgggaagtt acgcgagcaa tgagccgcgt aacttcccat ttaccatgtt gtaacaagtg
     9601 taattccaac aattttcccg gacaagcata aaaaccgctt gacagataaa aataaataac
     9661 gtattattgt attaaataaa taatacagta aaacaacaag acaatgaggg atccggggag
     9721 gcatcgatag aaacgatgcg tgacaggtaa taagaaacag aaaataagaa acaagaaagg
     9781 atgataggaa tggcgtggga attggatgct gacagaccca tatatgcgca gctggtggag
     9841 cggatccaga aagagattgt gtccggcaaa tatcagccgg gagagaagct gccgagtgtc
     9901 agagatcttg cggccgtggc ggcggtgaac ccgaatacga tgcagaaagc ttttgcggaa
     9961 ctggagcgga gcggcctgat cgtgacacag cgcaccagcg gcagaaccgt gacggaggac
    10021 actgagctga tcgggaatac cagagaacag ctggcagccg ggcatgtccg gaattttatc
    10081 aacaacatgc aggaactggg gtatcagaga gaggagatcc ggaatctctt cgataaggta
    10141 caggcctgac aggggtgcaa atttatgatg aaaataaaga gaggcggaaa tagaaatgaa
    10201 taatctggta gaaattgtaa atctgaccaa gaaatatggt gccaatgtgg ctgtgaacaa
    10261 tctgaccgta agtctcccca aggggaagat cattggcctg ttgggaccca acggcagcgg
    10321 taagacgaca ctgatcaaaa tgctgaacgg tctgctgaca ccgacggaag gcactattat
    10381 gatcgacgga aattatgtag gaccggtgac caagagcaag gtagcttatc tgccggaccg
    10441 cacgtatctg accatgaatc agacaatccg ggagattctg gatttctttc aggactttta
    10501 tacagacttt tccagggaac gtgcggaaga gatgttaaag agtctgggga tcgatcctgc
    10561 agtgaaaatg cggacattgt ccaaaggtac gaaggagaag gtacagttga tcttagtgat
    10621 gagcaggaat gccagcctgt atattttgga tgagccgatt gccggtgtgg atccggcagc
    10681 gagagattac atcctgcgca ccattatcaa caactacaat ccggaagcaa cggtactgat
    10741 ctctacccat ctgatcgagg atgtggaaca ggtactggat gaagtcatct tcatgcgtta
    10801 tggtcagctg gtgttatata catccgttga caatatcaga gaagagaaag gtaagtctgt
    10861 agatgcttac ttcagggagg tgttcgcatg ttaggaaagt taatgaaata tgaatggaaa
    10921 aatatctgga aagccggtac actgatgctt ctgggaatgc ttgtggtcac cgtgattggc
    10981 tgcgtggtat tacgcatgcc gggaggactg gtaacggaga ttgcggacgg caataacatg
    11041 aatgcgacac agagctggtt tgtactctct tcctttgtgg caacactgat cctgtatgtg
    11101 atcatgctgc tggcgtctac ctggggaatg ctgatttttt tgggcatccg cttttaccgc
    11161 agtatgtata cggatgaagg ctatctgtcc cacaccctgc cggtaacagc taaccagctg
    11221 tttttaagca aggtactggt ctccggtgtc tggtatctgt tcattacgat aggcatcggt
    11281 atttcagtgg tggcactgat cgtgtctctg atgacaggcc ttttaaatat cggtgaactg
    11341 agtagtgtac tgacacaata taatgggaat atctgggaat ttctggcaga tgctttttat
    11401 gaacttggca gaacatacga agaagagatg gggatcaatc tgttgcatta tgggatcacg
    11461 ctgctcttaa cctatgtggc aggaccgttc attaccatgg tcactctgtt cggagcgctg
    11521 accatcggac agctttcctc caaacataaa ggcctgatgg gaattctggc atatgccggc
    11581 ctgacgatcc tgtcgtccat catcggcagc accgtacaga gcgcattcat gtttggtgcc
    11641 aatgtcgcaa acagtgccag tggaatcacg gtgagcacca attccgccta tgatattaac
    11701 gtgatcacca gtctgctcat tgcggccatc atgtacggcg tatcttatta catcatgaac
    11761 cgaaaattaa acctggacta accgggtcta cagcttgccc tcaactggta aatttggtat
    11821 aataaatcca aatgacaaca gggaacgggt gaaggtacat gcaggagatt attgatagaa
    11881 ttttaggagg aaacttcgat tatgagaacg gttctttgga attctcctgc gctaaaatag
    11941 agatttccct gtcacagggg acaatatatg agggctcttt tcacattctt tctgcgtcgg
    12001 aaggctatgt aaaaggatcc gtgatctcgg accatcttcg gatggaatgt atgacggatc
    12061 agtttaccgg ctccgatgca gaaatttttt actgcttcca cggagaagat ctggaggaag
    12121 gggatgtgat caagggctct ttttccgtag tcagcaacag aggagaatac aatcttcctt
    12181 ttgtggtgac ggtggagcat gggatgttga attcttccat cggggctatc cggaatctgt
    12241 tccattttgc caacctggcg aagagcaact ggaaggaagc tgtgcggctg ttctatgatc
    12301 cggaatttct gcggctgttc caggggaacg atgcccattt ctatgacagt taccggatcc
    12361 tgtccactta tgagggcaac gaacaaaatg tggaggaatt cctgatctgt atcaacaaaa
    12421 agcaacagtt ggaattcctg acggaagaga aagaactggt gaaaaaactg cctcgcagcg
    12481 cagataatta cggcatcacg gagaacaatc tgaccatcgt gaggaacggc tggggatata
    12541 cgaatctcca gatcgagtgt gagggtgaat ttgtctttac ggaaaaggaa aacattacag
    12601 acgatgattt tctgggaaac cgttgccgcc tgccggtcta catcgacagc agcctgtgca
    12661 gatctggcaa aaatttcggt aaagtctaca tatataatgc ttatacttca ctggagatcc
    12721 cggtcatggt acagctggga gacggtgtag tggaccggca tgcagatcac agtcatatgc
    12781 agtgcatcac aaagatcatg aaatattatg aagaatcgag actgaaaaag atcggtacgg
    12841 gaacctggct cgcggagacc ggcaaactgg tggagcgcat ggtgaccatg gatgagaagg
    12901 atgtgccggc gagactgttc caggcacagc ttctgatcac agaggaacgc tacaatgaag
    12961 ccggatggat cctggatcac gcggcggaca tgttggaagc ccagggagct accggcggag
    13021 aacagtgggc ttactatctg tatctgacca cactgatcca cagggatccc cagtatacac
    13081 tgcaaatggc ggagcaggtg gaacagatct accgctatga caggacccgc tggcgtgtgg
    13141 catggctgct gttgtatctg tcggaggaat acaatcgttc aacctccggg aaatggatgt
    13201 tcttagaaaa acagtaccag tacggctgca ccagtccggt gatctacctg gaagcactgg
    13261 cccttttgaa cggcaatccg gcgctgctac gtaaattgaa cagttttgaa ctgcaggtgt
    13321 tgaacttcgg tgtccgtcag gatgcggtaa acgacagcct gatcgaacag ctgttgtatc
    13381 tgtcgggaag agtcagagag tattctccac tactgggtag aattttacga agactgtatg
    13441 agaaaaagaa ggatgtgcgg atcctgcagg aggtgtgcag ccttctgatc aagggaagta
    13501 agacgggacc ggatgccttc acgtggtatc agatgggtgt ggaaagccat ctgaggatca
    13561 cgaatctcta tgaatattat atggcgtcgg tagatctgga tgctgtgctg gagcttccga
    13621 aagtgattct gatgtatttt tctttccaga gcaatctgga ttatgaacat agtgcgttcc
    13681 tgtatgcgta tctgttaaaa caccggaaag attatgagga actgtacgaa cattacgagc
    13741 cgagaatgga gcggttcgtc attgaccaga ttcagaagca gcatattaac agacatctgg
    13801 caatactgta tcaggaattc ctgtctccgg ccattgtaac ggaagctatg gcgaagcctc
    13861 tgtcccgtct gttgtttgcc catatggtaa gagtggatga cagcagaatg cgtaaagtga
    13921 tcgtcagcca gccggggaat ctgatactgt ccgagacgcc tctgcaaaac ggcacggcct
    13981 gggtagcagt ctacgggaat gattatacca ttgcttttga ggatgcatat ggtaacaggt
    14041 tccttaaaaa tgtggaatat accctggaaa aactgctggt accggggaaa tatctcagac
    14101 ttctggaaca ttatgtgccg gatacggcgg aactggatct gtatttcatg gagaacggca
    14161 gaacagaaga gaccatatct tccacaaagc tgatgcggat ggcaagactg gtggaaagcg
    14221 atgccgtgga gccgaagctg cgcagtgaga ttgcggtaca gctggtacag gcctatttcg
    14281 acgcggataa tttgcaggca ctggatgaat acctgcagga gctgcagggg gacggcttta
    14341 cggaggaaca gcgggaactg atcctgcgtt ttctggtgtt gcggggaaat tatgagaagg
    14401 catatgcctg gatcgaagca tatacccctt attttgtgga ggccaagatt ctgttgcggc
    14461 ttacggacgg tgtgatcacg cagtcggtcc atgagggaga ggcagtcctg tatgcggcgg
    14521 cattgacggt gttccgcaag ggaaaataca acggtggaat tttagagtat ctggtgcgtt
    14581 atgccaccgg taccacgaag gaattgcgtg acatctggaa agcggcaaga tcctttgaga
    14641 tagactgcta tagcttaagt gaaaagatat tgctgcagat gctgttctcc ggggcatttg
    14701 tgggagagcg gatggatatt ttccgttatt atgtatccca gggagccaga caggagatcg
    14761 aggaagctgt gcttgtacag agctcttatg attatttctg ccgggagaag atcacggagg
    14821 aatatgtatt ccgggagatc cgtaactgct atctgcgggg agaagagacc cagcggatct
    14881 gcaagctggc ttatctgaaa ttctatgcgg agaataagga caagctggag cgggaagatg
    14941 aaactctggt ccggaatttc ctggaggaaa tgatgaagga ccacattcat ctgaactttt
    15001 tcagggaata tcaggactgc ctgccacagc tgcaggagat gaaggacaag actatcgtgg
    15061 agtatcacac aagaggcggc gtccgtgcaa ggattcatta tgtgatgatg catgagaacg
    15121 ggcaggcgga ggattacctg tcggaatata tgcaggaagt atacagcggt gtatttttca
    15181 aggaattcgt actgtttttc ggggagaatc ttcaatatta cattatggaa gaatcagaga
    15241 acgaagaaca gctgacggag agcggaagcc tgcagaagag cgatattatg aatgaaagtc
    15301 cggacagcaa atacgagatc atcaatgata tgatgatcag catgacactg caggacgata
    15361 cgacactgga tcatctgatc gaggaatatt accgcaggga atatctggat cacagattgt
    15421 tcacattgca gtaaagataa aaaaagacag caggagagga ggcggaagaa catgcgactg
    15481 ccgggaagtg ataaagttat agtgggttat gacctgggga aagattatgc gcagatcagc
    15541 tgctatgtca caggcaggga agaagagatc accacattgt cttccgttgc aggctcacag
    15601 gtctatacga ttcccctggt cctgagcaag agacagggag tcaaccagtg gttctatgga
    15661 agtgaagccc tccggcatgc ggaggaagaa gagggaatcc tggtggagca tcttctgaag
    15721 ctggcaaaag atggggaacc ggtgcagatc gatggaacga ctctggatcc ggtggcacta
    15781 ctgactcttt ttctcaagcg tagtctggga atgctgtccc agatgacttc cacagagcgg
    15841 atcggggccc tgatgatcac ctgcgaggaa ctggatgcag gaatgctgga ggtgctgacg
    15901 caggcagtgg aggcattaca cctgaagacg gatgctgtct gttttcagag ccatcgggag
    15961 agcttttatt attacaatct gtatcagccg gaaaatctgt ggaagcaggg gagcgtgctg
    16021 tgcgagtata gggacagttc catccagact tattatatgg aatgcaaccg ccatacgacg
    16081 ccggtggtaa cgtacatcga agagcgggaa ttcccttttc cggtggaaaa atcggatgaa
    16141 aaattcctgg agatcgccac acagctatgc gagaaccgga tggtgtcctc ggtatatctg
    16201 atcggggaag agttttctca agaatggatg aaagaatcgc tacgctatct gtgccgtgga
    16261 cggagagtct ttcaggggaa caacctgttc agcaagggtg cctgctacag catgatggag
    16321 cgactgcatc ccagtgaaaa cggacaaaat catgtctatc tgggacagga taaattgaaa
    16381 tccaatatcg gtatgaaagt gcgcagacag ggagaggaat cctatcaggc actgttagac
    16441 gccggaatca actggtacga ggcgcagaat accatggagt tttacctgct ggaaggaaga
    16501 gcggtggaga tcctgatcac gtccctgacg ggaaagggaa gccggatcgc cagaatcgtg
    16561 ccggaagaat tgcaggaagg aatcaccaga ctgcggattc aggtggagat gaaggatgaa
    16621 acacatctgc aggtggaact ggaggatctt ggttttggca gcttccgaac agctacgcat
    16681 catatctgga aggaagaaat agagttgtag atctgcccgg ggcagagaaa atgaggatga
    16741 aaaatgaagg caagtctgtg tgtaggagaa tattgcgaga atgcctataa tgtggaaggc
    16801 ctggatatcc gggtctatag tatggaagag ttgtgctatt gcctaaagga gaatgcattc
    16861 ctgttagacc tgtccatcat gaacgataag ctggtggact ggatcggaga agaatgtaaa
    16921 gtgtgggaac tggcaaaaca gctctacccc atggtacata agcagggatc cttaagtgtg
    16981 ttcgtggtga cgatcctgca atatgtgggg atgtacgatc cggaagaaat cctgcaggtg
    17041 gagcaggtac tgaagcaggg agccggactc agcaatctgg agaaacgcaa gagccagatc
    17101 gactatatgg tggaaaaaag aaaatatgcg gcagccatcc gtggatatga tatgctactg
    17161 gagacttgga accatctgga acaggaagga aaggaacttc cggcgggaaa agtccgggcg
    17221 gcgatcctgc acaataaggg tgtggcgctg acagggctga tgttttatga caaggcggca
    17281 tattacttta acgaagcctg gaagaccgat ccggacagag aacacctgga tgcttatctg
    17341 gcggcaaaga gaatggaact gacggaggat gcctatgtgg catttgcagc gcagaatccg
    17401 gaaaattata cggagtccct ggaactggaa aagcggatcg agcaatttga acgggaatgg
    17461 gaacagcagc cggagtaccg acagctacgg ctgcgtggtg actggcgggt gcacgacagg
    17521 gtgaaatacg atgcggaaaa tgagagactg actcaggcac tgaaaaacag ttaccggaca
    17581 agtgtcagcg tatagagaag acagcaggag agaattatgg cgtggtggaa ccgtttattt
    17641 aagaaaaaag aaccgataca gcaggaagag gactgggaac agctggtcta tacccgcaac
    17701 ggtgtgaatt ttgatgagga agaccagaga aaacgttaca ttgtaaattg cctggagcag
    17761 atctcagagg cttccaagga gatcgacctc ctgacgggag agtatacgct ggtgacttct
    17821 tatctgacgg atacggatga gattgaggcc ctgccagcag agcagagaga agaggtcaac
    17881 cgggtggccg gcaagctggc cgggctggaa caggaacgca gcagataccg ggaaaagaag
    17941 aaccgtatgc gggatgcgga cttctatgcg atccgcaaaa gagagaacga gattgaagaa
    18001 ggcattcaga agattcggga cggcgagaaa tacggcaatc tgataaggca ggatctgcac
    18061 agactggacg gagaacgaca tgcctacgaa taccggagaa atgagctgga taatctgatg
    18121 aacaaccaac ggggcatggc tgtcattttc cttacggcat tgatcgtctg tgtgatcatg
    18181 ctggtgatcc tgcagtatgc ttttgaaatg gatgtctatg tgggactttt tctggcagta
    18241 ggtgcaggag ctgtagccat ctcctatgtc tgcatccggt acatggatgc aggacgggaa
    18301 ctgcagcggg tggagaagac catcaacaag atcatccagc tgcagaataa agtcaagatc
    18361 cgctatgtga acaactgtca gctgatggaa tatctgtatc tgaagtacaa tacggacagt
    18421 gcagcaaagc tggaaaaaca gtggaaaatg tatcaggacg aaaaggaaga gcggaagcag
    18481 ttcgcagagg cggaagctaa gatcgagtac taccagaagc agctggtaca gcagctggcc
    18541 ggttaccgtg tgcagatgcc ggagcgctgg atcagccaga cagctgcgct tttggacaag
    18601 cgggaaatgg tggagatccg tcacgaactg atccagagaa gacaggtgct ccgtaaacag
    18661 atggactaca atcaggaagt agcggagagt gcaagaaacg aagtaaaaga catcgcagcc
    18721 acctatccga aatatgccca ggagatcctg gccatggtgg agcggtacga ccgataaaga
    18781 attgacaaga cacctctcgt tgtgttacta tgacaccgtg ttagcaattt accttgctaa
    18841 agtatacgac gcggccaaca gtgagtggca gaacgggagg atgcattatg aaaagagtat
    18901 tagtggcatt actggcaatc agcatggcag caggtgttct taccggctgt ggttctaaag
    18961 gatctgagaa aacatttacc gtaggttttg atgcggaatt tcccccttac ggatatcgtg
    19021 atgacagtgg agaatatgta ggctttgacc tggatctggc agcagaagta tgcaagagac
    19081 agggctggga gctggtaaag cagcctatcg actgggatgc caaggatatg gagctttctt
    19141 ccggagccat tgactgtatc tggaacggtt tcaccatgaa cggcagagaa gatgcctata
    19201 cctggacaga gccctatatc gacaacagtc aggtattcgt cgtagcgtct gcttccggtg
    19261 tacagaacaa ggaagaactg gcaggcaagg tggtagcagt acagaaggat tcctctgcgc
    19321 tggctgctct gaacgatgaa gaaaatgcag acaacattgc actgcgtgat tccttctccc
    19381 agctgatcga atatgcggac tacaataccg ctttcatgga tctggagcag ggtgcggtag
    19441 atgccgtagc cattgatatc ggtgtggcac agtatcagat tgcgcagaga gaagaaggaa
    19501 aattcgttat gctgcagggc gaggacaaca agctggctgt agagcagtat gcagtaggtt
    19561 tcctgaaggg taatgatgaa ttgagagata ccgtacagaa gacactggac gaaatagcag
    19621 cggatggtac cttcgcacag atcgctgaaa aatggggact gaccgacagc gtatgtctcg
    19681 gtaaataatc aactaaggat agttaaggag tagaattcat gagtttgctg accatgagtg
    19741 cccaactggc agagggcttt ctgataacgg tggaaatatt tgtactgacc cttttgtttt
    19801 cccttcctct ggggctgatc gtagccttcg gaagaatgtc aggatgtaag gtgatccgca
    19861 ttatctccaa aatatacata tccatcatga gaggaacccc tctgatgtta cagatcatcg
    19921 tggtgtattt tgcccccttt tatgtattcc gcatgcaggt gggaacgggg tatcgtttcc
    19981 cggcggtgat actggcattc gtgatcaatt atgcggcata ttttgcagag atctaccgtg
    20041 cgggcattga gtccataccg gtggggcagt atgaggctgc ggaggtgctg ggctacagca
    20101 aggcacagac tttcgtgaag atcattctgc cgcaggtggt gaagcgggtt ctcccgccag
    20161 tgaccaatga gaccattacg ctggtcaaag atacctccat ggcatttacc atttccattg
    20221 cggaaatgtt cacggtggca aagcagatcg gagcggcaca gaccagtgtg cttcccctgt
    20281 tggcagcagg tgtgttctat tatatcttca atctcgtggt ggcttctttc atggagtatc
    20341 tggagaagaa gacatcttat tacagatagg agcagtgcgg aaatatggtc cttttggaaa
    20401 tgaatcatgt gaaaaaaagt tttgacggca acggggtgct gaaggatata tccctgaagg
    20461 ttaacgaagg agaagttgtc tccatcatcg gcccctccgg ttccggcaaa tccacattgc
    20521 ttcgctgtgc aacgcttctg gagaaaatgg acggtggtga gctgatctat ttagggcaga
    20581 cagcagcttc ggagaaaacg gataaaaacg gcagaataca gtgcgaatat gcgtccaagg
    20641 cagaattaca taagatccgg aaatgtttcg gactggtgtt ccagaatttt aacctttttc
    20701 cccattatac ggtgatgaaa aatatcatcg atgcgccgat ccatgtggat aaggtaagcc
    20761 gcacagaagc cgtggcgagg gcggagaagc ttttagcaca gttgggactg tcggataagg
    20821 cagatgccta cccttaccag ctgtccggcg gacagcagca gagagtatcc atagccaggg
    20881 cactggcact gcagcccagg atcctgttct tcgatgagcc gacctcggca ctggatccgg
    20941 aattgacggc agaagtatta aaggtgatca gacagctggc aagtgaacat atgaccatga
    21001 tcatcgtaac ccatgagatg cagtttgcca gagaagtatc cgaccgcatt attttcatgg
    21061 aacagggtgt gatcgtggag gaaggttctc ccgaagcact ttttacaacg gaaaatgccc
    21121 gggttcggga atttatcgga aagttgcagt aaaaatatat tgattttttt tcgcaaatct
    21181 gtttatctat taggtgtggg tgtatcttgc ccacacctgt ttcaataaaa gactgaaaaa
    21241 cataagaatg cgaggactga tggtatgagc gtacaggaaa tgaaaccgat caaggaaggc
    21301 aaagttcgtg agatctatga caacggagat accctgatca tggtggcaac tgaccgtatc
    21361 agctgctttg atgtgatctt ggacaacaca gtaacaaaga aaggaactgt attgacccag
    21421 atgtcgaaat tctggtttga tatgactgag gatgtgattc ccaatcatat ggtatccgct
    21481 gatgttaagg atatgcccga atttttcaga cagccgaaat tcgacggcaa cagcatgatg
    21541 tgcaagaagc tgaacatgct ccccatcgag tgcatcgttc gtggatacat taccggaagc
    21601 ggctgggaaa gctacaagaa gaacggtacc gtatgcggta tcaagcttcc ggaaggtctg
    21661 aaggaggcag ataagcttcc cgagcccatc tatacccctt ccaccaaggc agagatcgga
    21721 gatcatgatg agaatatctc ctatgagcag agtgtggaat atctggagaa gcgtttcccc
    21781 ggcaagggcg cagaatatgc ggccaagctt cgtgactata ccatcgctat ttataaaaaa
    21841 tgtgcggatt atgcactgac caaaggaatc attatcgcag ataccaagtt cgagttcgga
    21901 ctggatgaga acggcaacat ggtactgggg gatgaagtat tgactcccga cagctcccgt
    21961 ttctggccgg tagacggcta cgagccagga catggccagc cttcctttga caaacagttc
    22021 gcccgtgact ggctgaaagc caaccctgaa aaaggctgga agctgccgga ggacatcgtg
    22081 gagaagacca ttgacaaata ccttcaggct tacaagatgc tgacaggcaa ggatctgtag
    22141 ggtacggttt tttagttttt tttcacgggg caggcaggaa aggtaaggct tacaagtgac
    22201 ggacaacatt tatattcaga cagaaaataa tgacaaatta aaggaagaat gtggcgtttt
    22261 cggcgtgtat gatttcgacg gacacgacgt ggcatccacg atttattacg gccttctggc
    22321 attgcagcac agaggccagg aaagctgcgg tattgccgtc agtgacacga atgggcccaa
    22381 gggcaaagtc ttaagcagca aagacatggg actggtcaat gaggcattta caccggaaca
    22441 gctggagaag ttaaaaggtg acatcggtgt gggacatgtc cgctattcta cagcgggagg
    22501 atccaccaga gagaatgcac agcctctggt actgaattat gtaaagggta ctctgggact
    22561 ggcccataac ggtaacctga tcaatgcgcc ggagcttcga agagaactgg agtataccgg
    22621 tgctattttt cagaccacca ttgattccga ggtgattgct tatcatattg ccagagagcg
    22681 tttgaattcc aaatcggtag aggaagctgt gggaagagcc atgacgaaga tcaagggagc
    22741 ctattccctg gtggtgatgt ctcccagaaa gctgatcggc gccagagatc cttatggatt
    22801 taagccgtta tgcatcggca agagagacaa tgcgtatttt ctgtccagcg agacctgcgc
    22861 tctggatacg gtaggtgccg agttcgtccg ggatgtgctt cccggcgaga tcgtgaccat
    22921 ttccccggag aaaggaattg agtccgatac cacccatgta ttaaagcctg cggagaccgc
    22981 cagatgtatt tttgagtata tttatttcgc cagacccgac agctttatcg acggcgtcag
    23041 cgtatacgat tcccgtattc tggccggaaa gttcctggct atggattccc cggtggatgc
    23101 ggatctggtc atcggcgtac cggaatccgg caactgcgca gccatgggat attccatgca
    23161 gtcgggaatc ccttacggca tgggcttcgt gaaaaatgcc tatgtgggca gaaccttcat
    23221 caaaccgaag cagaagagca gagaaagcag cgttcaggtc aaactgaacc ctatccggga
    23281 agccgtggag ggaaagcgtg tcatcatgat cgatgattcc attgtccgcg gcaccacctc
    23341 cgaccgtatc gtaaagatgt tacgggaagc cggagccaaa gaggtacata tgagagtcag
    23401 ttcaccgccg tttctgtggc cctgctattt cggcacagac gttccggcga gagaccagct
    23461 gatcgcatat aaccgttcca tcaaggagat tagtgatctg atcggcacgg acagcctgga
    23521 ttatctgaag attgagagac tggaagaact ggtgggcggt aatcttggca tctgcaaggg
    23581 ctgctttacc ggaaaatacc cgatggaacc gcctgcggaa gatatccgcg gagaatttga
    23641 caaataaaca ataactattc agaaaaccgc agaataagaa aaactgatac gtcatgcctg
    23701 catgtaagta gcagttttct tatttgtaag gtgactcgag tagagtcacc atcccacaca
    23761 tgaacaaaat tagctgcgaa gcagcgggaa agtatgagaa gcatacggtt ttgtgaatgt
    23821 gagggatggg ttttgaatgg ttgcaataaa ttagaaaata agaggagcag acaaatgagt
    23881 acaggtacag acagatatca gagcccgtta tccgaacgct atgcaagcaa ggagatgcag
    23941 tatattttct ccccggatat gaaattccgt acatggagaa agctgtggat tgcactggca
    24001 gagacggaga aagagctggg actgtcccag gacggtaagc ccgttatcac agatgagcag
    24061 attgaggaac tgaaggccca tgctgaggac attaattacg atgtggcaaa agagcgtgaa
    24121 aaactggtac gtcatgacgt tatgagccat gtgtatgcat acggacagca gtgccccaag
    24181 gcagccggaa tcatccatct gggcgcaacc tcctgctatg tgggtgacaa caccgacatt
    24241 attgtgatga ccgaggcact gaagcttgtc agaaaaaagc tgatcaatgt tatgaaggaa
    24301 ctggcggatt tcgctgataa gtataaggca cagcctaccc tggcatttac tcattttcag
    24361 cctgcacagc ctacaaccgt aggcaagaga gcaacgctgt ggttacagga gttctcactg
    24421 gatctggagg atctggatca cgttctgggt accatgaagc tgttaggctc taagggaacc
    24481 accggaaccc aggcttcttt cctggaactg ttcaacggag atcaggagac catcgacaag
    24541 atcgatccta tgattgcagc caagatggga ttcaaggagt gctatcctgt atccggccag
    24601 acttattccc gcaaggtgga taccagagta gcaaacgtgc tggccggtat tgcggcatct
    24661 gctcataaga tgagcaatga catccgcctg ttacagcatc tgaaggaagt ggaagagccc
    24721 ttcgagaaga accagatcgg ctcctccgcc atggcttaca agcgtaatcc catgagaagt
    24781 gagcgtattg cttccctgtc ccgttatgtg atggtggatg cgctgaatcc cgccattact
    24841 tccgcaaccc agtggttcga gagaacgctg gacgattctg ccaacaagag actgtctatt
    24901 cccgaaggct ttcttgccat cgatggtatt ctggatctgt gcctgaatgt ggtagacgga
    24961 ctggtagtat atccgaaggt aattgaaaag cacatgatga gcgagcttcc tttcatggct
    25021 accgagaata ttatgatgga tgccgtaaag ttaggcggca accgtcagga actgcatgag
    25081 cgtatccgtg agctttccat ggaggccgga cgcaatgtga aggtagaagg aaaagagaat
    25141 aacctcctgg agctgatcgc cgcagatccc gcattcccca tgggactgga ggaactgaaa
    25201 gcatccatgg atccgtccaa gtatatcgga cgttccaaag agcaggtgga agctttcctg
    25261 aaaaatgtga tcagcccgat cttagaggcc aacaaggact tactgggtgt aaaggccgag
    25321 atcaacgtat aaacgttttc tgctgaagta atttgtggaa acggaaacta ttcagaacct
    25381 cattcatcgg tgatcttttg atgtttacat ggctctgaat cgttacagaa tgataagaaa
    25441 gcaggagacg aaaatgggtg gattttttgg agtcgcagca aaagaagact gtgtgtttga
    25501 tctgttcttt ggaacggatt atcattccca tctgggaacc agacgtgccg gcatggcagt
    25561 ctacagcaaa gaagaaggtt acaacagagc cattcacaac atagagaatg caccgttccg
    25621 tacgaagttc gacaaggatg tcaatgagat gcacggacat ctgggcatcg gctgtatttc
    25681 cgattttgag ccccagccgc tgatggtgcg ttcccatcac ggaacttatg ctattaccac
    25741 cgtgggtaag atcaacaata cggatgccat catcaaggat ctgtttgcga agggacattc
    25801 tcatttcctg gagatgagcg gcggtgacat caatgccacg gagctggtgg cggccattgt
    25861 caaccagaag gacaatctgg tggagggcat tcagtacgcc caggagatca tcgacggttc
    25921 catgacctta ctgatcatga cacccaaggg aatctatgcg gccagagata agatgggccg
    25981 tactccggtg gcagtaggca aaaaggaggg ggcttattgc gtctcctttg agagcttcgc
    26041 ttatctgaat ctgggatacc agcccgtgag agagctggga ccgggagaga tcgccgtggt
    26101 gacaccggaa ggtgtacgaa ccctggtgaa gcccggcaag gatatgaaga tctgtacgtt
    26161 tttgtgggtc tattacggat atccgtcctc atcctatgaa ggcatcagtg tagagaagat
    26221 gcgttatcaa tgcggtgcca agctggcgga gcgtgataat gtaaaaccgg atatcgtagc
    26281 aggagtgccg gattccggta ccgctcatgc agtgggatat gccaaccgct cgggaattcc
    26341 tttttcccgt ccgtttatta agtatacccc cacctggccc agatccttta tgccgacgat
    26401 ccagacccag agaaatctga tcgcaaagat gaaactgatc ccggtacatg atctgatcca
    26461 ggatcagagt ctgttgttga tcgacgattc tattgtccgc ggcacacagt taagagagac
    26521 cacggaattc ctgtaccaaa gcggtgcaaa ggaagtacat atccgtccgg cctgtccgcc
    26581 tctgttatat ggctgcaagt acctgaactt ttccagatct tcttccgaga tggatctgat
    26641 cacccgccgt gtgatcaagg agattgagca ggagggcaga gaaatcgatc tgaagaacta
    26701 tgtgaatccc gatactccgg aatacgagga gatggtagga cggatctgca aacagctgaa
    26761 gttcaccaca ttgcagtttc agagattgga tgatatgatt gagtccgtgg gcatcggcag
    26821 agagaagctg tgcacctact gttgggacgg cagagaataa tgcgaaaaga acttctaatc
    26881 actcagagca taggctcttt cgtgaagaag ttctaagttt cgcatggcaa aacgctacgc
    26941 gttttgaata tagatgtagg ttatataaaa tataataaat attgatttta cttataccat
    27001 aaagtgttgt atactgtaaa ggtgtgagaa agagaagcag gagtttcttt ggatcacccg
    27061 gaataagata ttggatgtag atttatcaca gaaactgtgg taaagaatgg aggaaaacat
    27121 ggtaaaggca gttgtaggag ccaactgggg cgacgaagga aaaggaaaga tcacagatat
    27181 gctggcagag aaagcggaca ttatcgtacg ttttcagggc ggcgcgaatg caggacatac
    27241 catcgtgaac aattatggta aatttgctct gcacaccctt ccttccggtg tattttacaa
    27301 tcataccacc agcgtgattg gtaacggtgt ggcattaaac attccgatcc tgatgaaaga
    27361 gatcaagtct atcacagaca gaaatgttcc catgcccaag attctggtgt ctgatcgtgc
    27421 acagatggta atgccttacc atattttatt tgatcagtat gaagaggaga gactgggcgg
    27481 taagtccttc ggatctacca agtccggtat cgcacctttc tattctgata agtatgcgaa
    27541 gatcggtttc caggtaagtg agctgtttga tgaagaactg ctgcaggaaa aagtacagcg
    27601 tgtcagtgag atgaagaatg tcatcttaga gcatctgtat cacaagcccc tgatcgatcc
    27661 cgcagagctg ttaaataccc tgcatgagta tcgagatatg atcgcacctt ttgtatgcga
    27721 tgtatccgca ttcttagata aggcactgaa agagggcaag accattctgt tagagggcca
    27781 gttaggaacc atgaaggatc cggatcacgg aatttacccc atggtaactt cttcctccac
    27841 gttggcagct tacggcgcca tcggtgcagg tatccctcct tatgagatca agcagatcgt
    27901 taccgtaagt aaggcatatt cctcttccgt aggagcaggt gctttcgtat ctgagatctt
    27961 cggtgacgaa gcagatgaat tacgtcgccg cggcggtgac ggcggtgagt tcggtgcaac
    28021 taccggacgt cccagacgta tgggatggtt cgactgtgtt gccaccaagt acggctgtcg
    28081 tctgcagggc gctaccgatg tggcatttac cgtactggat gcactgggat atctggatga
    28141 gatccctgta tgtgtagggt atgagatcga cggtgaagtt accactgatt tccctgttac
    28201 ctacaagctg gaaaaggcaa agcccgtact gaaaaagctc cctggctgga agtgcgatat
    28261 ccgcggtatc cgcaacttcg atgaactgcc ggagaactgc agaaagtatg tggaattcat
    28321 tgaggagcag atcggattcc ctatcaccct ggtcagcaac ggcccgggaa gggaagatat
    28381 catctatcgt gagagtcctt tgaagaaata aatgctgtca aatgcggaga gataggataa
    28441 tctttgatgg aaaaaacatg tgtaaaatgt gaatgagaat ggaaagggca tggtgctggt
    28501 atgagatcag cccgtgtcct ttttagtata cgatgaatcc ggcaggaatt ttgattacac
    28561 agtatctggc tgaatgcaag cattcgcaga acgcagtttt gcatggctct gaatagttac
    28621 agatttttac tgtgaaggaa caaaaaccag tgtctgatag taattttgag atggagacag
    28681 cgagatatga tcaataatct ggaagcgtat aaagtatttt attatgtggc aaaatgtggg
    28741 agtgtgacca aagcggccgg ggaactgtca atttcacagc cggcggtcag tcaggccatc
    28801 aagcagctgg aaaataccct ggatgccgct ctgttccacc gggcggccaa gggagtacgg
    28861 ctgaccagtg agggagaact gttgtattcc tatgtggcaa aagggtacga acagatcgaa
    28921 atgggtgtga agaaggttca ccagatgcag aatatggaac tgggagaagt ccgcatcggt
    28981 gccagcgata tgacattgca gttttatctg cttccctatc tggaaaagtt ccatgaacag
    29041 taccccggga tcaaggtgat cgtcaccaat gcgccgacac cggagacatt gaattattta
    29101 agagagggac acattgactt cggaattgtc agtacgccgt ttccggagag acctgatatt
    29161 cagatgatac cggtgcggga gattgaggat gtcttcgtgg cgggaagaag atttttcagc
    29221 tacaagaacc ggacgctgga ttttcagaaa ctggaaagtc tgcctcttat tttcctggag
    29281 aaaaatacca gcagccgcag ctacatggat gaatttctgg cgggaaacgg ggtatatgtg
    29341 cagccggagt tcgaactggc cactagcgat atgatcgtac agtttgtgag acgaaatctg
    29401 ggtgttggct gtgtgatgcg ggattttgcc agagattatg tggaatccgg cttgcttttc
    29461 gaacttagat tcaataaaat aatacccaag cgtcaattct gtgtcgttag gaacacgaag
    29521 atgcctatgg ctccggctgc ggagaaactg ttggagatca tggagctgga cagacaggaa
    29581 gaagcgaaga tataaaagtg acggcaaaaa gaggatatac cggcggaaac ggaactgctt
    29641 tgactagtaa aacgaatagg agagaagagt tatgatacgc aatataattt ttgacattgg
    29701 caatgtgttg acagactatc gctgggaggg atttctggcg gacaagggat ttgatgcgga
    29761 aatgatcaaa aaaatcgcaa aggcatctat tatgcatccg ctgtgggcag agtatgaccg
    29821 cggtacctgg acggacgagc aggttctggc tgcatttgta aaggatgcac cggaactgga
    29881 gaaagaactg catcaggcgt tcgagaatat cgctggcatg gtaactccca gagcttacgc
    29941 tatttcatgg ttacaggaac tgaaagcaaa gggataccat gtgtattatc tgtccaactt
    30001 ctcacacaag gcagagacag aatgtgcgga agcgttgaat tttctgccgg agatggaagg
    30061 tggcatcctg tcctacaagg atcagctgat caagcccgaa ccggagatct accagctgct
    30121 tctgaaacgc tatggattaa aggcagatga gagcgttttc ctggatgata cgctggtgaa
    30181 tgtgaaggcg gccgaagaac agggtatcca cggcatccac ttcctgacca aagagcaggc
    30241 ggaagaggaa ctgcgtaagc tcggcgtgga ctgctaaatg gttcgtagcg gagggtgcct
    30301 tagaccggac gaactcccag agcggcagaa agccctcagg cagatatcgg tctccgagtg
    30361 tgaattttct aaacccgtgg atcaagtatt tctcatccaa acgggcacgg ctccaggcga
    30421 catccatgtc gccgaccgcc tgtcgcaaac atcgtgtttg ctccaccctg actttttacc
    30481 acaggttaag aaaatatcaa cactctccga cagacatctg cctgagggct ttctgccact
    30541 tcgggagttc tgtttgtatt caaaacgcca atttctacga aaaaaataca aaaacataag
    30601 attttgctat gtaaaaagtc tctattttcg ctacacaagc aaaataaaga aattccgtac
    30661 aaaaaatcct gatttcggaa cttttttgta cggaattcgt tgttttgggg ctttttgcac
    30721 caaactaact tgctatttat tttcgttctc cttttcttcc ggatggaagt ctgcgaaggt
    30781 ggagcggttt gtcagttttc caaacatcca ggtgtagatc cagatgagaa gagggatggc
    30841 cacggtggct agcaggcagg tggcgaatag cgcgtggctg gcggagctgt cgaagatcgc
    30901 cgcaaaaagc gtcacgacat ataaaagcac cagcagaata attccaatga tcgcgaagat
    30961 ctgtttggaa gtcatttttg ttttcttgtt ttgtgtattt ttatcctgtg acatttgtat
    31021 tcctttcgtt attcccattc ataattcaca aaagagcgnn nnnnnnnnnn nnnnnnnnnn
    31081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31141 nnnnnnnnnn nnnnnnnnga gcggaagtgc cggtgcggtt cgtggacctc aaaagagcgg
    31201 aagtgccggt gcggttcgtg gatcgcaaaa gctgcgggag tgccggtgca gttcgtggac
    31261 ctcaaaagag cggaagtgcc ggtgcggttc gtggatcgca aaagctgcgg gagtgccaga
    31321 actgtccgtg tgcctcaggg actgcggaac cgtcagagtt attcgtgatt ctcagaggtc
    31381 tcggaactgt gttccacatg ttcttcgttg ggttctggaa tcgtcatcag taccttgatg
    31441 atacggttct ggtcgatacc ctgtaccttc aagtgaatac cgttctccag ggtaacttcc
    31501 tcaccatcct ccggcagacg atccagacag tcgatgatca gaccgccgat ggagtcataa
    31561 tcttcactgg tcagatttgt tccaagaacg tcgttgatgt catccagctt catgctgcct
    31621 tccaccagat aagtatcttc tgacatcttc tggatcagtt cttcctcgtc ctcatcgtat
    31681 tcgtcacgga tctcaccgac gatctcttct aacagatcct ccagagtgat cataccaacg
    31741 gtagcaccgt attcatttaa tacgaaagag acattggtgg ttttttcccg catctcatac
    31801 atgaggtctg cgattttctt gtactcgtaa gtatagtaag catcacgcaa aatgtttccc
    31861 acatggaaag cactctgatc ctccgtcagg ataaagtcct tgatattgat cagaccgata
    31921 atattgtcct tgtcatcctc atagaccgga agcctggtat acatggaatc ccggaaaact
    31981 gccagaacat cgtcataagt agcatccaga ctgaccgtta ccatattgat gcgtggaatc
    32041 atgatatcct tggcgtgggc atcggaaaaa tcgaacacat tgtagatcag cttgcgttcc
    32101 tcggattcga tgacaccgtc ctcatgactg acgtccacgt aggttttcag ctcgctttca
    32161 gtcatggtgc tggctttttt gttgggatcg atatgtaaca gcttcatgat accgtgggac
    32221 agcagatcca caatgaagat gaccggcgtg agcacctgca tcagaaaata gattacattg
    32281 caatatgtca gggccagctt ctcagaatac agtattgccc aggtcttggg tacgatttcg
    32341 ccgcagagca gcacgacaat ggtcagaaca ccggttgcga tacctacagc cagattgatt
    32401 cgcatagcta atgttgttgc cagggaagat gcagtcagat tgacaatatt gttgccgatc
    32461 agaatggtac tgatcatttt actgtaattg tccaggactt tgttgacgcg tgcagcacgc
    32521 ttgctgcctt cctcttccag ggtacgcatt cgtacacgat tgaccgtgga cagtgctgtc
    32581 tcggcagaag agaaaaagcc ggatagaaaa atcagaatta cgatagaaac aagttgtatg
    32641 acaccagagg tatccaaaaa agttcactcc ttcttaaata atagtagtag gcataagccc
    32701 catttataag ttattaagca acaatataca ataggacagg aatagttgtc aagttttccg
    32761 aatgatttga atatatattt tacataggta aaaaggagga gcaacatgga aaaaagaggc
    32821 gagagaaaag aaggggttcc ggtgcttgca ctggcaaaat gtctgctggc atcctatgtg
    32881 ctgacactgt tgcttttatg tctgttggca cttttattgt ataaattgca tctgacggaa
    32941 ggaatggtga ccattgcaat ctcggctatt tatgtcgtaa ctacctttat ggcgggattt
    33001 ttggcaggaa aaaagatgga gacgcagaag tttctttggg gagcactgct aggggcagca
    33061 tatttcgtga tactggcggt ggtatccgta ttgatcaaaa cgccggaaac tgcattgggc
    33121 aattccttct ggaccacaat ggtattgtgt gtggccgggg ggacactggg cggaatgtta
    33181 gggtaaaata cagaaatgga caggaattta acggtttttg agaaaaaaag cttttacaaa
    33241 tgccaaatac atgatataat gagcaaaaat gagttgcgtg tctgtatgtt tatatacacg
    33301 ctggcgtcta taggcgcttt gaaatcataa ggaggctatt tttaatgaag catattaaga
    33361 ctttaacaac cagagattta aaggaatcca tgaaaaaggg cggctgcggc gagtgccaga
    33421 cttcctgcca gtctgcatgt aagacttcct gcactgtagg aaaccagagt tgtgaaaaga
    33481 ttaaataatc taagaaaatg agaagagact aacgtaagca gcgattcagg tcgctgctta
    33541 ttctctgtta actattcgga gctggagatg acagcgtatt ctttgtcgga tgcttgcata
    33601 cgtaagaaga tacgtccggc aaaatacggc gaagtaacca catgagcaaa taacatgcag
    33661 gcatgttatt caggaaagtc cggaggatga ttttgcgaat ggggttccga tagttaatgt
    33721 taatgtattt taagcaataa gaagtagaaa gaagaggaat agaactgtga tacaccagta
    33781 taagagcaac ggctataata tcgttatgga tgtgaacagt ggttctgtcc atgtggtaga
    33841 tgatattgtg tatgatatga ttccgctggt agagcctctg gtgacagaag gtatcaagga
    33901 tgcggacacc atcaaggctg ctgtactgaa tcttgcaaat atcccctatc cccaggaaga
    33961 gatcaccgaa gcggtggacg aggtcctgga actggagaag gcaggacagc tgtttgctcc
    34021 cgatatttat gagaattaca tttttgattt taagaagcgt cagaccgtag tcaaggcact
    34081 gtgcctgcac attgcccatg actgtaacct tgcctgcaga tattgctttg cggaagaggg
    34141 tgagtaccac ggcagaagag cactgatgag ctttgaggtc ggcaaaaagg cactggattt
    34201 cctggtagcc aattccggca accgtgtgaa cctggaagtg gatttcttcg gcggcgaacc
    34261 actgatgaac tgggatgtgg taaagcagct ggtagaatac ggcagaagct tagagaagtc
    34321 caacaataaa aagttccgtt ttaccctgac taccaatggt atcttattaa atgatgagat
    34381 cctggagttc gtgaataagg aaatgggtaa tatcgtgctg agtatcgatg gacgtaagga
    34441 gatcaacgac aagatgcgtc ctttccgtgg cggacagggc agctatgaca tcattgttcc
    34501 caagttccag aaggtagcgg agagcagaga ccagatgaac tattatgtgc gtggtacgtt
    34561 cacccacaat aatctcgatt tctccaagga cgttctgcat ctggcagatc tgggattcaa
    34621 acagatctcc gtggagcccg ttgtggcaca gcctacggat gattatgcca tcagagaaga
    34681 ggatcttccg atcttaaagg aagaatacga taaactggca gtagaaatga tcaagcgtaa
    34741 gaaagagggc aaggctttca atttcttcca ctttatgatc gatctgcagg gcggtccctg
    34801 cgtagccaag agactctccg gctgcggatc cggtaccgaa tatctggcgg ttactccctg
    34861 gggagacttg tatccctgcc atcagttcgt gggtaacgag aagttcctca tgggcaatgt
    34921 ggataccggt gtggtaagag atgatatccg tgacgagttc aaatgctgta atgtctatgc
    34981 aaaggacaag tgtaagaagt gttttgccaa gttctattgt agcggcggat gtgctgcgaa
    35041 ctcctataat ttccatggca atatcaatga tgcgtatgac atcggctgtg agctgcagag
    35101 aaagcgtatc gaatgtgcta tcatgctgaa ggctgcggaa gcagcagaag cttcagaaga
    35161 atagaaagga caaaggaaag atgaaaaaaa gtaaagcaat catcattctg ctctgcgccc
    35221 ttctggtcct gctgggtgtc ggctatgtgg atctgtatgg cgtagatgcg gaaggaactg
    35281 ccagtgcttc agacataagt ctgggactgg atctggccgg tggtgtcagt atcacttatc
    35341 aggtggtagg cgatgaagag ccggatgcaa cggatatggc agatactatt gccaagctgc
    35401 agaagcgtgt tgagaactac agtaaagagg ctattgtata ccaggagggt accgatcgta
    35461 tcaatatcga gattcccggt gtaacagatg ccaacaagat cctggaagag ctgggacgtc
    35521 ccggttccct gtatttcatt gcagagacag acaaggacgg caatgcgaac tactctatgc
    35581 aggcagtgac cgatgcacag ggcaatgtaa gctatgcgta cgctctgaat aagaccatcg
    35641 atgaactgaa ggcagatgga tccatcatgc tggagggtac agatgtcaag actgctaccg
    35701 caggctctat caaggaccag acgatgggca attccactta tgcggtagat ctggtgatga
    35761 cggaggaagg cactaagaag tttgaagagg ctaccagaaa tgcttacgag aagggcgaga
    35821 ccctgggtat ctactatgat ggatcctttg taagtgttcc ttccgtaaaa ggtgtgttct
    35881 ccgatggtaa tgcacagata tctcctatga actcctatga agaggcagag agcctggctt
    35941 ccaccatccg tatcggtgga ctgaagctgg agctggaaga gctgcattcc aaggtagttg
    36001 gtgcacagtt gggtgtggaa gcaatctcca ccagtttaaa agcagctgtg atcggattta
    36061 tcgtagtggc tgttttcatg attgcagtat atttcctgcc cggatttgct tctgtcattg
    36121 cactgggtat ttatgtggca ttggtagtat tgctgctgaa cggctttgac ataaccctga
    36181 ccctgtccgg tatcgcaggt atcatcctgt ctatcggtat ggcagtggat gccaacgtta
    36241 tcgtctttgc ccgtatccgt gaggaactgg caacaggcaa gactgttaag agtgctatgc
    36301 agatcggtta tgacaaggca ctgtccgcta ttatcgacgg taacgtgact accctgatcg
    36361 cagcggcagt gctgtggctg ttaggccccg gtactgtcaa aggcttcgca cagacgctgg
    36421 cacttggtat cgtattatcc atgttcactg cactggtagt gaccaagtac attatgaaag
    36481 ctttctacgc actgggactg aaagatacta agtggtatgg tatcggcaag gagcacaagc
    36541 cgatcaactt cctcggcaag aagggtattt tctttggact gtctctggca gtgatcctgg
    36601 ttggattcat taccatggga gtatataaag taaacagtgg tgatgcactg aactacagtc
    36661 tggagttcaa gggcggtacc gcaactactg ttacattcaa cgaaagctat accctggatg
    36721 agatcaatag ccagatggtt cctctgatcg agagcaccac cggcagtgca gtacagatcc
    36781 agaccgtgca gggttccaat gaagttatct tcaagaccaa ctctctggat gtggatcaga
    36841 gacaggctct gaatcaggta tttgtggata acttcggcgt agatgagtct ctgattactt
    36901 ccgagaccat cagctccacc atcagtaatg aaatgaagtc cgatacgatc ctggcggtag
    36961 cagtagcaat catctgcatg ctgatctata tccgcttccg tttcagcgat atccgtttcg
    37021 gtgtcagcag tattgcctgc ctgctccatg acgtgctggt agtgatcacc ttctacgcac
    37081 tggcgagagt atccgtcagc aatactttta tcgcgtgtct gctgaccatc gtaggttact
    37141 ccatcaacgc caccatcgta gtattcgacc gtgtacgtga gaatatggca gtcatgacga
    37201 agaaggatga cctgcaggag gttgtaaacc gcagtattac gcagacatta tccagaagct
    37261 tatttacctc ccttaccacc ttggtaatgg taggtgctct gtacatctgg ggtgtgacca
    37321 gtatccgtga ttttgcattg cctttgatgg taggtatcat ctgcggtgct tattcctccg
    37381 tatgcgtggc aggtgctctg tggtatgtac tgagaaagaa attcgcgccg aaggcaaaat
    37441 aagatttcac atagtagaat ggaatcaaaa aaggagtcgg aatgaaatgt tccggctcct
    37501 tttaaagtta agttatggtt atacattctg ctgcaaactg gcaagttcct ttttcagaga
    37561 ttcaatctct gcgtctttcg cggcattctc ggcagtcaga gtatcaatct tcttcagcag
    37621 atactgttcc tggagttcag catccattcg tgcctggcag cgttgtctga ccatgtcatc
    37681 cgcattcaga ttatataaag tctttgtcgc ttccatcagg tattcatcat ttttagcagt
    37741 cataagaatt tcctcccatg aaatagaccg gaataaagca gcccatttat caatgccata
    37801 tgctttgtca gcgtcagtag ccaattctat atgctttaag tctaccacag aaagtgtgag
    37861 tttgtcacta tattcaaaat gatccttaag attcagtaat cgatatgtag catagaactc
    37921 agggtgttcc gggaacaggg tgtagtctaa aaaaccaatg tgaattacag gttttaattc
    37981 agaataattt tgtcctttat ttagttgatc aaagttacgg cacagataac tcagggaacg
    38041 ttccggccag ttgtataggt tattcacctg catttccaaa tttatgagag tattattgtt
    38101 caacaagaca ttggtatcga gaacaaaatc tttgtcttca atagttgcac ctaaagtgat
    38161 tggattggta atggtgacgg acttaattgc ggatggtggc agatggagca gagaactgat
    38221 gagaccgatc agagcctttt gacttagctg cagggcagcg cggaacatat agtcattgcg
    38281 gaaaccgtag tcaataggac cggtaaggtt catggaaaat acttgtttgt gtttcatagg
    38341 cagaatcctc cttagtgtca ttaaaagttg cttgaaaaat ggcgaaacgc cggatgtaag
    38401 aaatttaaaa tagaaattga attgactata gcataaaaag agaagatgca caagaatttt
    38461 tatggtacaa tgtaatgagt ctcagggaaa aacaatcggc tgtgaagcag aatgtagagt
    38521 gcgaaagcac gaatacgtga gcaaaattat gaaagtggga aaagtatgga aaaatggtat
    38581 gtagccgcaa aaaaggcaga ttttgataaa tgggcgaagg aatttcatat cagtccggtg
    38641 acggcgagaa tcctaagaaa ccgggatctg acggaagaga gtgagataca gaagttcctc
    38701 tacgggaaac tggaagattg ttattctccc tggctgttaa aggatatgga taaggcagtg
    38761 gaggaaattc taaaagcggt aagggaaggg ctgcatatcc gggtcatcgg ggattatgat
    38821 gtggatggaa tctgttcttc ctatattctg accaaaggtt ttcagatgct gggggctcag
    38881 gtggacacgg ctattcccca cagaatccat gacggctatg gattgaacga gcatctgatc
    38941 gaagaggcca aaagagaagg tgtcggtatg atcgtgacct gcgataacgg aattgcagcg
    39001 gcaccccaga tcgcaatggc aaattctctg ggcatgcggg tgatcgttac ggatcatcat
    39061 gaagttccat atatagaaga aaacggagag cggaaggaac agctgcctcc ggcgctggca
    39121 gtggtggatc ccaagagagc agacgatacg tatccttttt ccggcatttg tggtggcgtg
    39181 gtagcactga aactgatcca ggcgatcacg gaaaagacag gaaatccggg acttgtaaat
    39241 attcaggagg agttactgga atttgcagca ctgtctaccg tatgcgatgt tatggagtta
    39301 aaagatgaga atcgcattct ggtgaaggaa gggttgaagc gtatccagaa gggatataac
    39361 gaaggcttga aggcgctgat ggaagtgaat gatattgcgc cagatcggct gagcgcctat
    39421 catctgggat ttgtgctggg accctgcctg aatgccacgg gaagactgga tactgccaaa
    39481 cgggcattgg aactgctgca gagctttacc agggcggatg ccatgaccgc ggcgagagag
    39541 ctgaaagacc tgaatgagag ccgcaagaac ctgacagtac agggaattca gagagcggat
    39601 gagtatattc aggaacatca tatggcagaa gataaagtta tggtgatcta tctgccggag
    39661 gtacatgaga gcattgccgg tattattgcc ggaaaagtca gagaaaaata ccatcatccg
    39721 gtcttcattc tgaccaaggg agaagatgga gttaagggtt ccggacgatc catagaagcc
    39781 tatcacatgt atgatgccat gacgcaggta aaagagtatt ttaccaagta cggcggtcat
    39841 aagctggcag caggcctttc catgcgagaa gaggatattg aaccgctgag agctgctttg
    39901 aataaaaaat gcactttgac ggaggatgat tttattccga aagtacatat cgatgtggca
    39961 atgcccatgg ggtatgcgga tgatgcgctg gcgggagagt ttgccctgtt ggagcctttc
    40021 ggcaccggga atccacgtcc tttgtttgcc cagaaggggc tggtcttcca ggcgggcttt
    40081 aaaatgggtg ccaacaagac ctgtgccaga ttccgggtga agacaccgga gggtacgaca
    40141 cagacggtgg tattttttgg agatctggag aaactgggag ctttcctgga tgaaaaatac
    40201 ggtgcaggaa gtgaagaggc actttatgcc gggaaaggga atttcccatt gtccgtggta
    40261 tatcaggtca gccagaatac ctataagggg aaaacagaag tacagttcgt catgcagaac
    40321 tattgttaaa ttttttattt tttgaacaca aaatggacac attttctctg taatatgtat
    40381 ttcagatagt tgatgaactc actatctccc cccctcataa gaaaccgaaa agccttaggt
    40441 acgatgcctg aggctttttg gtgtccggag agaaacttac cgcagtttat aaaacctcaa
    40501 gacacatttt agaattaagt tagaattcgt acacactctg tacacattta ccctgtaata
    40561 tgtatttcag atagttgatg aactcactat ctccccccct cataagaaaa tcgtaaggcc
    40621 tcaggcgcaa cagcctgggg ctttacgatg tctggaaaag caaaatagtt tctgataatg
    40681 cttgacacca cccgaaagtt atggattata atataaaaat atggatgatg ccagggtcaa
    40741 aaggtctaag cccacatgag cttgctcata ggtgggtga
//
LOCUS       DAWEJW010000010        39918 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_457_length_39918_cov_57.620550, whole genome shotgun sequence.
ACCESSION   DAWEJW010000010 DAWEJW010000000
VERSION     DAWEJW010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 39918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 39918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..39918
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_457_length_39918_cov_57.620550"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15758..15857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        11476 a         8463 c        11191 g         8688 t
ORIGIN      
        1 gcacggaaca atccgtaggc atcaaagtcg ggggcttttg accccgacat catatgaata
       61 aaaattataa aaatatagaa ggaatagcag cacaaaaatg aaaacacaga tttacgaatg
      121 gagacaggca ctgaaaaata ttaccttaaa aaattgtgcc ataactttgt tgggaagttt
      181 tattctggca tttggattat ataacgtgca ctccatctcc ggagtaacgg agggtggtgt
      241 gctgggagcc acgttgctgt tggagcattg gacaggaatc tctccggcgt ttaccggagg
      301 gattatgaat attttatgtt atatcctggg atggaagctg ttaggcaagg aatttatcgc
      361 ctattcggct ctggctaccg caggattctc aggaacctat aaaatctgcg agcaatttcc
      421 tcatctgtgg ccggaactgg caaatatgcc ccttctggca gcactggtgg gagcattgtt
      481 cgtcggtgtc ggtgccggac tgtgcgtgcg catcggcgga gcccccagcg gagatgatgc
      541 cctggccatg agtatttccc atgctaccgg ctggaagatc cagtgggtgt atctgatgtc
      601 ggatctgatc gtactggtaa tgtccttaag ctacattccg gtgagacgta tcggatattc
      661 gttgtttacc gtgcttatgt ccggacagct gatcggcctg atccagaact tcggaagtgc
      721 atccaacagt gatgcggagg gtcttgcgac ttccaagtgt gaaattatgg attgatctgg
      781 taaatctgaa tttctccgtt gtcaaagata cagggccagg cggagcggaa gtagttttca
      841 tcgacagcgt aacttgcccg ttcccaggaa gagacttcaa tataatcgat gtcgtaagta
      901 cggatcagat cagcagaaac agcgggagtc tcataaattg tccggaaatc ttcgtgatat
      961 tctgtgtgat agagaccgtg cattgccaaa aaggttcccg ccccgtttaa cacattgcgc
     1021 cctgcaaggg cggcaatttc atttacatgc ctgttgttgg taagaaaacg ggatgtggga
     1081 gaggtatttt gctcgacgaa tgctgcagct tccacctgtg cggaagaata agcggtataa
     1141 tcggatatta cctctctgca cagggtcaga acggcagaaa aggtgcaaag aaataccatg
     1201 ccgatcatgc agaggtatcg tgaagaggct tttttcagag aacgcaggaa atcaattccg
     1261 taatcggcac tgaggcagca gacaaatata taagttatat ataatagttt attattgtca
     1321 tatgtgttag gggtgaagga aatgatttcc acaacaaacc acaggagaaa tgccccggac
     1381 atcacatcaa aatcttcttt tttacaatga atcatggcag ggacgaacca tagcaggatc
     1441 gcaccccagt ttttcagata aaaccacaaa taagtatcgc cctgatttcc ccagttaaaa
     1501 tgccccagca aaaagccgga ttgcgttgtc tggccaaaag tccattccaa taactgcgga
     1561 agtgcaagaa gcagaagcac accaaagaaa actccccagg tggtcaaaaa cttctgcagt
     1621 gtatcattgt ttgtactttt accggaaaag caccggtaca gtaaggacag accgtatagc
     1681 acgagggatg ccgcaacaag gatgccaaaa cgaaagagca gtacaggtgc aacggcagca
     1741 gaactttcgt tcagaatttc cagaagaatc atacaggtca cgaaacatcc gagcagaacg
     1801 gctcccggat gctctaccgg ggaagtatta tgcggcacgg accggtaaag gcacagcaac
     1861 atccaacagg cacttaagat caggatggca acgaaggaat gggtatggat cataggtaat
     1921 gcccctgcca ggatgccggc agggagaaaa cattcttttt ccccgttccg gatggcccga
     1981 agcagcagcc agatggcaca gaacaggacc gcatagccaa agagtgttgc acgctgtggc
     2041 aggagcatat ctgcgatgat attgacccag cggatattcc ggtcgaccag attggtgggg
     2101 gtagtgtaat agccggtaaa aatatcactg aatttataac cgccttctct gctccagtca
     2161 atgaaatata cgaagccaag accgccgtta tagaaaaata agacccaggc agccagagat
     2221 ttggcgcgat catggaacag aacatcggca atggcataaa atcccgttat gacctggaaa
     2281 aaagcaaaat acatagggag catataagca tagcgcaggg atgcaccaaa caggtaaata
     2341 ctggaagaaa tactgtcgca caggaaagga tagcagagcc tatcccaggg agagatggag
     2401 tagtaaggag ggaaggtctg ttgattggca atacttgtaa tgattcccag gtgcatctgc
     2461 agatccccat aagtacactg ccccacatga agaccgtcgt tcttcggcag gatggtgtgg
     2521 gtggataaca aaatacaaaa cagaacaaag gtaccgcctg ctagcagaag aaaggcataa
     2581 tttctgcgca acagctgccg gtagtctttg gtgggagcag tcgaagatac tgccgtggca
     2641 ggatggtaaa agacatatag caggaccaga gaaatgacta cgaaaagcag agccataata
     2701 tgagctgtca tggaaaaacc gaagaagaag gcaaacagag caggaatcca ctgcaacaga
     2761 acagagccgg atacactgcc ggtcagcaga tggaacagaa gtcccttttt aggaaaaata
     2821 cgttttatgt acagaaaccc cacacattga aatagaagaa aataaagtgt gccaaggata
     2881 tttccgaatg cagatccata gaaaccgttc atgagaattc ccccttaaat aaaaatcagt
     2941 aatattatac cattgcttcg gaaaaagcaa aagatgcaac taaatttaag ggtggtacat
     3001 tctgagaaaa aagagtaaaa taaaatacaa tatgtgaaaa agattcctga gagaaaggcg
     3061 gatacctcta tggaaaagga taagaataag atggaaaaca agaaattaac agagcttctg
     3121 gagcacttgg aatatgagct ggtacaggga acactggaca gagagatccc tgcggtggtc
     3181 tatgattccc gcaaggtcgt tccgggatgc ttatttctct gcatcggcgg agcaaatttt
     3241 gacggtcatg attttgctgc acaggtggca gagcagggag ccggagtact agtggtgcaa
     3301 aaagacgtgg aactgccgga gaatgtggat gtgacggtga tcaaagtagc ggataccaga
     3361 tatgccatgg catttatttc tgcggcctgg ttcggccatc cggcagaaaa gctgaaagtc
     3421 atcggtatca cgggtaccaa aggtaagaca acgaccacct atctggtaaa gtccattctg
     3481 gaaaatgccg gctataaagt gggactggtg ggaaccatcg aagtgatcat tggagacgaa
     3541 catatccatg ccaacaatac caccccggaa tcctatctgc tgcaggagta ttttgcccgt
     3601 atggtggagg caggactgga tacggttgtg atggaagtat cctctcaggc gctgatgctg
     3661 caccgtacgc agggctttgt gttcgattac ggtattttca cgaatctgga accggatcat
     3721 atcggtccca acgagcatgc cagcttcgag gaatatctgc actgcaaggg attattgttt
     3781 aaacagtgca aggtaggtat tgtgaatggt gacgatgagc actggcaggc agtgacggaa
     3841 ggacatacct gcacactgga gaacttcgga atgggagagc actgtatgct ccgggcggaa
     3901 gaacggaaac tggtgcataa gcccggagaa ctgggcgtga ctttccacgt agccggactg
     3961 atggattttg atgtggaagt gcccatgccc ggcaaattca gcgtgtacaa tgcgctggcg
     4021 gctattgcca tctgccgtca tttcaaggta gatgaggaaa acattaaaaa ggcactgttg
     4081 caggcaaagg taaaaggccg tatcgagatg atcaaggtat ccgatcagtt caccctgctg
     4141 atcgattatg cccacaatgc catggcgctg gagagtctgc tgaccacgtt gcgggaatat
     4201 gaacctcaca gactgatcag cctgttcggc tgcggaggca accgttcgag acagcgtcgt
     4261 tttgagatgg gagaggtcag cggcaagctg gcggatctga ccatcattac ctccgacaat
     4321 ccccgttttg aggaaccgca ggatattatc gacgatatca agacaggtat ggcaaaaacg
     4381 gatggaaatt atgtagagat ctgcgatcgt aaggaagcca tcacttatgc tatcgaacac
     4441 ggagaacccg gagatattat cgtgctggca ggcaagggac atgaagatta ccaggagatc
     4501 cgcggggtga agcatcccat ggatgagaga gatctgattc gggatattct ggcggaagga
     4561 catattccgg gatctgcagc aaaataatga ggaaaacaga ttgaatacgt tatatgcgga
     4621 tatcattgta gatatctccc acgaaaaact ggatagaacc tttcaatacc gggtgccgga
     4681 gagtctgcgg ggcagactgg agccgggagc ctgtgtcatg atcccgtttg gcaacggcaa
     4741 tcgtcttatc aagggttacg tggtggcttt gggggaaacg tgtcagttcg ctccggagaa
     4801 gatcaaggat atcgcaggaa tgccggaaaa agagaccgga gtggaggaca aaatgattgc
     4861 tctggctgcc tggatccgtc ggaattacgg ctgtaccatg atccaggcgc tgaagacggt
     4921 actgccggca aaacagtcgg taaaaaagct ggaacacaga cagctggtac gtctgatgaa
     4981 cagagaagag attttgtcat tattaggaga atgtgaacgg aaaaaacagg tggccaaagc
     5041 aagactgtta aaagccctgt cagaacagga gacgattccc tatgagtgga tcaccggaaa
     5101 actgggggtc agtgcagcca cggtaaacag tattgtaaaa agcggcgcgg caaaactggt
     5161 cagttccgaa agctatcgca atccggtcaa aatgcagtcc gtagaggcag tgcgtaatac
     5221 gctgagtgag gaacagcaga ccatcgttac ggaagtgctg aaggattttg atgccggaag
     5281 gagacagacc tatctgatcc acggcatcac cggcagcggc aagacggaag tatacatgcg
     5341 gctgatcgga gaaatgatcg cgaggggacg gcaggctatt gtgctgattc ccgagatcgc
     5401 cctgacttat cagacactgc tgcgatttta ccagagattc ggtgatcggg tcagcgtgat
     5461 gaattccacg ttatcccccg gggaaaaata cgatcagtgc gaacgggcca aagctggaga
     5521 aatcgatgtg atcatcggcc caagatccgc actttttacg ccgtttccaa gtcttggtct
     5581 gatcgttatg gatgaggagc aggagaacag ctataaaagt gaaagtactc cgaaatatca
     5641 tgccagagag actgcgctgg aagtggcaaa actgtacggg gccagtgtag tgctgggttc
     5701 agccacacct tccctggagg cctattatcg tgccggtagg ggagaatacc ggctgtttca
     5761 actgacaaag cggctcaccg gcggggaact tcccacggtg tacacggtgg atctcagaca
     5821 ggaactgcag gagggtaacc gttctatttt cagcagaaaa ttgcaggaac tgatgacaga
     5881 ccggctgaac aagggccagc agaccatcct gtttttaaac cgaagaggct acgcgggatt
     5941 tgtgtcctgc cgttcctgcg gtgaggtcat gaagtgtccg cactgtgatg tctctctctc
     6001 ggaacataag ggaggaaggc tgatctgtca ttactgtggt tatacacagc cgatgccgaa
     6061 gctttgtccc aagtgtggat cgaaatatat tctgggattt aaggcaggta cccagcagat
     6121 cgaggaaaag ctgaaggagc tgtaccccgg tgtgcggacg ttacgcatgg atgcggatac
     6181 cacccggacg aaggacagct atgagaaaat attatcctcc ttcgcaaacg gggaagcgga
     6241 cgtgctgatc ggcacccaga tgatcgtcaa gggacatgat ttcccaaagg tgacactggt
     6301 ggggatcctg gcggcggatc tgtcgttaaa tgcgtcggat taccgggcgg gagagcggac
     6361 tttccagctt ctgacccagg cggtgggccg ggccggcaga ggagcgctcc ccggggaagc
     6421 ggtgatccag acctatcagc cggatcatta cgccattacc tgtgctgcgg ctcaggatta
     6481 cgagagcttt tatcaggagg agattctgta tagagagctg tccggctacc cgccggtggc
     6541 tcatatgctg gcagtacaga tctacgggaa agaggaagaa aatgtaaaac agcttgccct
     6601 gagactgaca gatgtggtaa agtctaatat ggactttttg tcaggaaagc aaaaagaaca
     6661 gtccaataag aagatacagg tactgggacc tgcccctgct aacatcagca agatcaatga
     6721 tatctaccgg tatgtgttct atgtgaagca tgcggagtat gatgtcctgg tggaagtaaa
     6781 ggacagactg gaggaaactc tcagacagtg gcagcccaga cagatgagta tacaatttga
     6841 ttttgacccg gtaagcgtgt tataggaggt aaagaactgt ggcaatcaga aacattcgtg
     6901 aatatgggga tgaagtactg accaagacct gcaaagaggt caaggagatg acaccccgga
     6961 ccaaagaact gatcgaggat atgttggaga ctatgtacga tgcaaatggc gtagggctgg
     7021 cggcaccaca ggtaggtgtc ctgaagagaa tcgtggtgat cgataccacc ggagaggatc
     7081 cgtatattct gatcaacccc cgcatcgtag agagcagtgg agagcagacc ggacaggaag
     7141 ggtgtcttag cgtacccggt aagttcggca tcgtaaccag acccaattat gtgaaagcgg
     7201 tagcactgga tgtgaatatg aagcccatcg aagtagaggg aacagaattg ctggccagag
     7261 ccatctgcca cgaactggat catctggacg gtcatctcta tgtggaaaag gcggaaggac
     7321 cgctgcagga tatgacctac gaggatgagg aagaagagta agctttccgg aaaggagaca
     7381 gactatgcag atcgtattta tgggaactcc cgattttgcc gtgggagcgc tggaggcact
     7441 gatcacacag ggccatgaga tcaccgcagt ggtgacccag ccggacaagc caaagggcag
     7501 aagcaaagaa ttacagtttc ctcccgtgaa ggaatgtgcg gtaaaacatg gcattcctgt
     7561 attccagccc cgtcggatca agacaccgga agccattgag gaactgaaaa aatatcccgc
     7621 agatatttat atcgtggcgg cgttcggaca gatcctgtct caggagattc tggatctgcc
     7681 gaaatacgga tgtctgaaca tccatgcatc cctccttccg aaataccgcg gggcatctcc
     7741 tatccagaga gtgatcatcg acggagaggc cgagactggt gtgaccatca tgcagatgaa
     7801 tgcgggactg gacaccggcg atatgttgta ccggaagaaa atccccatta cttcggagga
     7861 caccttcgaa actttgcatg acaagctgat ggtgctgggc ggggaagcga tcaccgaggc
     7921 actgcctctg ctggaggcag gcaaactggt accggaaaag caggatgata ccaagacctg
     7981 ctacgcggcc ctgatcacca aagagatggg aaaactggac tttacgaaaa cagcagcaga
     8041 actggatcgt ttgatccgtg gactgactcc ctggcccagt gcatatacgc attatcatgg
     8101 aaaacagctg aaaatctgga aagccattcc tatggcaact gcgcataagg aagcaccggg
     8161 cacgatcctt acggtgaata aggattcctt tgaagtggct gcatcggaag gctccttaaa
     8221 ggtgatggaa ctgcagctgg aaggtaaaaa gcgtatgaca actcacgatt tcctgctggg
     8281 ggtaaaggta cagccgggag agcttctggg cgagcgttag gatacgacga aacgaaaagc
     8341 aacgatcggg taagcggtgt tgacagaaag gagactttcg tatgtacggt ggctattacg
     8401 gatatggtta ttattgggat cctacttatt ttctggtatt ggtcggactg gtattgtgtc
     8461 tgggtgccag cgcactggtg aattccacca tgcgcaaata cagcaaagtc cggaatatga
     8521 gaggattgac gggagcggaa gcggcccgca acattctcaa cagagaaggc ttatacaatg
     8581 tacagatcga gtgcctgaac tccgatgacg gtgatcatta cgatccccgg acggatacgg
     8641 tgcgactgtc ttacaacaac tttaacggaa cctcggtgac cgcggtcagt gttgcggccc
     8701 atgagtgcgg acatgccatt cagcatgccc atggctatac ccccatgaac attcgtgctg
     8761 cactggtgcc ggtggtgaat atcggaagca atttaagcat tcccctgatc tttttaggag
     8821 tactgctcag ctggaaccag acattgatcc agcttggtat ctgggcattt gccctgaccg
     8881 ttattttcca actggtgaca ctgcctgtgg aattcaatgc ttcccacaga gctatcgtga
     8941 aggtggaaga atacgggtta ttaggcagtg aagaagtaag cggcggcaaa aaggtgctga
     9001 cggcagcggc tctgacctat gtggcaggag cggcctcctc cgcattacag ctgctccgac
     9061 tgatcctgct gtacggcggc aatcgcagaa ggagagacta atcttgagtg attatgcaac
     9121 gaccgataca agagaaatta tagtggagat cctgctcagt ctggaaaagg ggcaggattt
     9181 ttcccataaa ttgatgaaag cggttctgga taaatacgat tatctggatc ccagagagaa
     9241 agcatttatc aaaagagtca cggaaggtac gatagagaga cagctggagc tggactatta
     9301 tctggatcgg ttttcttctg ttccggtccg gaagatgaag cctctgatcc gctgtcttct
     9361 tcgcatgagt acttatcaga tcctgtatat ggacgcagtt cccgacagtg cggtgtgcaa
     9421 tgaggcctgc aaactggcgg ccaaaagagg cttccgtacc ctgaaaggct tcgtcaatgc
     9481 ggtgctgcgt aatatttccc gcagcaagga acagatgccg ttgccggatc ccaaggatac
     9541 ggtgaaatat ctgagcataa aatattccat gccggaatgg atcgtgaatc tgtggctgcc
     9601 tgcatatggc agagaaggaa cggaaacact tttaaaagga ctcctttcca ttcatccggt
     9661 atccctgcgg ttctccacgg aactgaccga atcggagaga gagtcactgg cggagaaaat
     9721 tgagaaaacc ggtgtacgtc tgcagcaaag cagggaactt ccttatgtgt atctggcaca
     9781 gaatctggaa aatgtaagtg aacttccggg ctttgcggaa ggcaaattta ccgtgcagga
     9841 tgccagctcc gcacttgcag tggaagcggc aggtatatgc ccaggtaata cggtcatgga
     9901 tctgtgtgcg gctccgggtg gcaagacgat cctggcggcg gaatttgcag gggaagcagg
     9961 ccacgtcgtg tcccgtgata tatcggaata caagacggac ctgatccggg agaatctgga
    10021 gcgcatgaac cgtaggaatg tggaggttca ggtgtatgat gccacggtgc atgatccgga
    10081 gaaggaaaaa tatgcggacg tggtgctgat ggatgtcccc tgctctggtc ttggtgttat
    10141 gggaaaaaag agagatatca aatatcatgc cacaccggaa agcctgcaat ccatcacaga
    10201 actgcaaagg cagatcgtgg cagccggatg gcagtatgtg aagccgcacg gtgtactgct
    10261 ctacagtacc tgcacgatcc accgcgggga aaatcaggag atggcagcct ggatcacaga
    10321 gaatttcccg tttgtactgg aagaagagag acagcttctg ccgggaacgg atgagaccga
    10381 cggattctat tatgcaagac tgcgcagaaa agcgtgagag ataagaagta agagaacgaa
    10441 aaatggatac gaaaaaagat atcaaatccc tgaatctgga agaattgaag acagagctgg
    10501 agagcatggg agagaaggcc ttccgggcaa agcagatgta tgaatggatg catgtgaagc
    10561 tggtcaggag ctttgatgaa atgaccaacc tctccgcaaa attcagggag caatgtaagg
    10621 aaaaatatga atacacctgt gtaaaccctg tcagaataca ggagtccaag atcgacggaa
    10681 cgaaaaaatt cctgtttgaa ttatctgatg gcaatgtggt ggaaagtgtc tggatgcagt
    10741 ataagcacgg caacagtgtg tgcatttctt cacaggtggg atgccgtatg ggctgtaaat
    10801 tctgtgcgtc cacgctggac ggactggaac ggaatctgac tccttccgag atgctggatc
    10861 agatttacgc catcacaaga ctgacggggg agcgggtctc caatgtggta gtcatgggca
    10921 ctggggaacc gatggataat tataacaata ttttgaaatt tctgcatctt ctgaccgatg
    10981 agaacggtct gaacatcagc cagagaaatg tcacggtgtc cacctgtgga atcgtgccca
    11041 gaatgcggca gctggcggat gaaaagctgc agatcacact ggcattgtcc ttgcatgcca
    11101 ccaccgatga aaaacgaaga aaactgatgc ccattgccaa ccggtattct atcaaagagt
    11161 tgatggaagt ctgtcagtat tatttcgaac agaccggacg aaggatcact ttcgaataca
    11221 gtctggtggg cggtgtgaat gatacggaag aggatgccag ggaactgatc gccctggcga
    11281 agccgttgtg ctgccatatc aacctcatcc cggtcaaccc cattaaagag cgggattatg
    11341 tgcagtcgga tacgaagcac attcaggcat ttaaaaataa acttgaaagt aagatgatac
    11401 atgttacaat tagaagggag atgggccggg atattgacgg agcctgcgga cagttaagac
    11461 gccgtcatac gcaggaggcg aagaacccgg gggaactctc acaggaggaa gtttgacgtg
    11521 cttaagacgt tttccataac cgatatcgga aggaaaagaa aagtaaatca ggactatatc
    11581 tatacctctg aaaaaccggt gggaaatctg ccgaatgtat ttatcgtggc agatggtatg
    11641 ggtggtcaca atgctggaga ctatgcgtcc aaggtaacgg tagagaccat gcttgccgag
    11701 atggagaatt cttttgagaa gaatcccacc ctgatcttcc gcaaagccat tgaggaagcc
    11761 aatgatgtga tccgcaagag agcaagcgag gatgaaaagc tggaaggaat ggggactacg
    11821 gtcgtggtgg catcctgcat gggcagattc ttacaggtgg ccaatgtagg agacagcaga
    11881 ctctatgtgg tgggcagcaa gatccgccag atcacaagag atcattcgta tgtagaagaa
    11941 atggtacgga tcggagaact ggatcgtgcc gatgcaagga accatccgga taaaaatatt
    12001 attaccagag cggtaggggc ggaagacaaa gtggagccgg atttctttac cgtggaatta
    12061 caggagggtg agatcgtcct tatgtgctcg gatggtctga caaatatgct ggaagacgag
    12121 gaaatccgca tgattatcag cggagccaga gaccttgtgg agaaggcaga gagcctggtg
    12181 gaggcggcca atgccaacgg cggcagagac aatatcagtg tgatactgat cgagccgtgc
    12241 gcgtaaatta cccagctttt ttggaaatgg agagcaagta tgatcaaaat aggaatgatg
    12301 atcggcgatc gctatgagat cctggaaaaa atcggaaccg gcggtatgtc agatgtctat
    12361 aaagcaaaat gtcacaagct gaatcgctat gtggcgatca aggtattgaa gcaggaattc
    12421 agcgaaaatg ctaattttgt atccaaattc cggatcgagg cacaggcggc agcaggactg
    12481 atgcacccca atattgtcaa tgtctatgat gtcggggaag agaacggaat ctattatatc
    12541 gtcatggaac tggtagaggg tattaccctc aaaaagtaca ttgagaagaa ggccagactg
    12601 tcttacaagg aagcggtaag tatcgccatc caggtaagca tgggtattga ggctgcacat
    12661 aataatcata ttatccatcg ggatatcaag ccgcagaata ttattatctc caaggacggc
    12721 aaagtcaagg tgacggactt cggtattgcc aaggcagcta ccagtaatac catcacttct
    12781 aatgtcatgg gaagtgtaca ctatacttct cccgagcagg ccagaggggg ttacagcgat
    12841 gaaaagagtg atatctattc gctgggtatc accatgttcg agatgctgac gggaagagtt
    12901 cctttcaacg gggagactac ggttgccatc gccatcaagc atattcagga agaaatgccg
    12961 tctccgaagg agtttgtacc ggagatccct tccagcgtag ccagtattgt actgaaatgt
    13021 tgtcagaagt ctccggaccg cagataccag aatatggcag aactgatcgc ggatctgaag
    13081 cagtccctga tcaacccgga ggaagatttc gtggtgaata ccgatccgga tatggaaggt
    13141 ggtacccgga tgatctccga tgaggatatg gcgcagatca aacgaaaagt cccttatcag
    13201 ggaggacggg aatacgaaga accggatcat gacgagacca tgcgtctgcg ttccgatacg
    13261 gaagccatgt atgaaaacga agaagatggc gaagacgaag ataattacga ctacgacccc
    13321 aagatggagc gtgtgaccac aatcctggca attgtagccg gtgtggtgat ctgtattatc
    13381 ctgatcgttc tggcagtgaa gatcttcggc ggcaaaaatg aaggaagcag tgactctgat
    13441 gcaggaccga tcattacgac cgaagagacg gatgccacag aagtggaaaa ggtctccatg
    13501 ccgaatgtaa cgggcatgaa ggtagaagat gcaaagagca ccctgaccag tctgggaatc
    13561 ctcacagagg tgacttatga ggagtccgac acggtagcgg aaggtattgt tatcaataca
    13621 gatgtcagcg tgggagcaca gatcgataag ggcagcaagg taaccctgaa agccagtgca
    13681 ggaactcagg gtgtggaagt acccaatgtg gtgaatctta cttttgatga agccaataca
    13741 cagctggtaa gcaagggatt cctggtcaac aagacagaga actattccga tacggtagag
    13801 tccggccggg tgatttccca gaccccctcc gagggcaaca aagcaccaaa gggcagtgtg
    13861 atcactgtga cagtaagtct gggtaaggaa gataccaaga tccgtgtgcc gaatctgatc
    13921 ggtctgtccg agcaggacgg cacggtagaa gccatcgagg caggactgac cgtaggcagt
    13981 gtgacctata tctacagtga cgaagtggga gaaggcatga tctgttacca gagctattcc
    14041 catggctcct atgtggagca gggaacttcc attgatatca aggtcagtca gggagctcag
    14101 aaggtgactt acaagtgtaa tgtcagcatt gcggcaccga cgacttcgga agcacccggc
    14161 tatgtatcgg gaatggaagt cagcatcaaa ctggtgacgg atgacaataa cgtactgctg
    14221 gataccaaga ccagcacttt cccggtagct accaatttca acggtatgac tgcctccggt
    14281 ggtacgctca ccataacgta tcaggtgaca agcgaaccga ccaccacaac gaacccggat
    14341 accggggagc aggtgacggt tcccggaacg acagaagacc gttcctttac cagaagagtt
    14401 gagttcgtaa aggaataaga tcaaaggcgg gaagctatga agggcaagat tataaaagga
    14461 atcgccgggt tctattacgt ccatgtagcg gacgatggcc atacaatggg cacggtgtac
    14521 gaatgcaagg caaaaggagt ctttcgcaag gatcacagaa aacccctggt cggggatgac
    14581 gtggagatgg atgtcctgga tgaggcacag aaaaaaggaa atatcaggga attgcttccc
    14641 agacacagtg aactgatccg tccggcggta gctaatgtgg atcaggcgct ggtgatcttt
    14701 gccatcacga agccacagcc gaattttaat ctgctggatc gtttcctggt catgatgcag
    14761 cagcaggaca ttccctgcat tatctgtttt aacaagcagg atattgacga ggaaggcaaa
    14821 aaggaagatt atcgtgccat ttacgaacag gcgggattcc gtacgatcac ggtcagcgca
    14881 gccagacaag aagggataga taccattcag gagctgcttc gcggcaagac cactacggtg
    14941 gcgggtccca gcggtgtcgg taaatcctct ctggtaaact gcctgcagtc cggcacagtg
    15001 atggagaccg gctccatcag tgagaagatc gagcggggaa agcatactac caggcactcc
    15061 gaactgatct ccattgcaca ggatacgtat attctggata ccccgggatt tagctccctg
    15121 gggttgtttc ggttggagaa ggagcagctg gcggaatttt atccggagtt tgcccctttt
    15181 gaaaaatatt gcagattcgg aggctgctcc catatctctg agccggtatg cggtatcaag
    15241 gctgctgtgg cagagggaga gatcccgcag atacgttatg agaactactg tctgctctat
    15301 gatgaactga aaaatcagag aaaatattaa ttatgagtga agtgcagaat aatctagggg
    15361 aaaaattcaa agagaccatt gcggaagcaa tgaagaccgg agattttcat gcgttgaaca
    15421 cgctggtgac ggatacggtc aacgatgcct tgcgggaggc ggatttccag gcaaatctgt
    15481 ccagagaggc cacggagaaa aaggtgtgga attcccaacg cagctggcag gataaaaagc
    15541 gggaggaagt caagaccgag gcggagatcc aggcggaacg gcagcagcgg gaaaagcagc
    15601 aggaagagca ccgcagacag aacgaggaat ggcacaggca gcgcagagag cgggaaagac
    15661 agtgggaaga acagcggaag gcgcaggcag cacagaaccg tgcacggaat caggcacagc
    15721 cggtgacacc cagaaaagct actgcggcag aaatgacnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnctg tgcggcacca aaatgtatgg tgtgatcaac gaaatggccc
    15901 gctataccgg aaaaagtgtc cgctatgtca gaagggatct tcgtaagatg ttaaaagccg
    15961 gaatgtttcc ggacggacat ctggatgata aagagacctg ctttatgctc aataatgtga
    16021 tctaccgtca gtatctggag acggagaaag cctatctgga gcagaagaag gaacagaata
    16081 cagcgggcac cgatacacag gatacggagc agaaccgcag agagcaggaa ctgcacagta
    16141 ttgtggcaga aggaatggaa tacatccgtc atctgcggga tctgaatgac aggatcgaag
    16201 gagaagctat ctctgagaag ctgtaccagc tggaaaatct gttgaaggag atctttgacc
    16261 gagtgcagga gcatccggag cagatgcacc ggatgcataa actgatggat tattacctgc
    16321 ctactatgtt gaaactggtg gagcagtatg cggaatttga cgagatcagc agcccgggag
    16381 aagatattct cgatgctaaa aaagagatcg aaaagacact ggatatgatc aatcaggcat
    16441 tcgttacatt gctgaacaat ctgttccggg atgcagcctt tgatgcgtca gcggatgcac
    16501 aggtattgca gacgatgttg gccagggaag ggctgacgga atcggatttt gtaagagaaa
    16561 aaagtgtatg acagcaggaa agcaatgctg atggtaaata aaaaatcatg agagagtatg
    16621 gaaaggcggg gttataagta tgaatatgga tctggatgca gcaacaaaag aggcacctac
    16681 ccttacactg gatccctttg cggaggcaaa ggcagagatc gtggagaaga agccggagga
    16741 actggtagag gagcaggctg ttccggagat ggaactgaca ccggaagaac agaaaatggt
    16801 agatgatttt gcgggacaga tcgatctgac caatacccag gcggtattgc agtacggtgc
    16861 ggggagtcag aaaaagatcg cagatttctc tgagacagca cttagcaatg tccggaccaa
    16921 ggatatgggc gaggttggac agctgttgac ggatgtagtg gcacagctca aggattttga
    16981 tacagaggaa gacaagggat tcttcggctt gtttaaaaag agcggggata agctgagtaa
    17041 tctgaaagca aagtatgata aagcggaagt gaacatcagc aagatctgtg atgccatgga
    17101 aaatcatcag gtggtgctgc taaaagatgt ggcggtactg gacaagctgt atcagctgaa
    17161 tctgaattat tttaaagaac tgtccatgta cattctggca ggtaaaaaga aactgacaca
    17221 ggcgaaaaac gtggaactgc cggaacttct ggaaaaagca cagaagagcg gactgccgga
    17281 ggatacgcag gctgcgaagg attttgcagc aatgtgtgag agattcgaga agaagatcta
    17341 tgatctggaa cttaccagag ccatttctct gcagatggca ccccagatcc gactgatcca
    17401 gtccaatgat atcgctatgt ccgagaagat ccagtccaca ctggtgaata cgatccctct
    17461 gtggaagagc cagatggtca ttgccatcgg tctggatcat gccaccgatg cagccaaggc
    17521 acagcgtgca gtatcggata tgaccaatga actgttgaag aaaaatgcag aagcactgaa
    17581 agtggcaacc gtggagacag cgaaggaatc agagagaggt attgtggata tcgagaccct
    17641 caagaccacg aaccagacac tgatgaccac actggatgaa gtcatgaaga tccaggagga
    17701 aggccgtgcc agaagacaga gcgcagagga agacttacag cggatcgaaa atgaaatgcg
    17761 ggataagctt ctggagatga gccagtcatc ccatacatga tttttacata attttaagca
    17821 gagaaatctt gacgaaacaa ccagaggaaa ttaaaataaa atgtatgtag gttaattaac
    17881 ggaaggagca ggaaacaaat gaaaaaactg gaagaaatgc taagggagaa aacgattaaa
    17941 aataagcttt cttttgtgtt ccgcattatg gacgttttat atgtggtgct ggcactggtg
    18001 gcagttgtgg gattgataca gtcaaaaaac tatgtgggaa gcgtaattgt tgtgatcata
    18061 gttgtattag ctattgcatt taatgcagct atgtcaaaga tgcttgccaa aatgttggta
    18121 gagcctattg agagtctggt agtggcatct gagaagattg cttccggtga ctttgagatc
    18181 ggcactcctt atgagtccga ggatgagctg ggacgcttat ccgacagttt tgagaccgca
    18241 gccggtattt tgaaaaaggt tgtatcagat ctgcaggata ttgtagagca ttttgctgag
    18301 ggtaattttg atgtacatag caactgcccc gatgcgtatg taggacagct ccgcaatgta
    18361 ctggatgaac tggatgatat ggtgaacaag gtcagtgaga ctatgcacgg tattcaggga
    18421 tcttccgaac aggtgtctgc cggaagcaat cagctggctg tcagtgcaca ggatattgca
    18481 gaaggtgcta ccagtcaggc tgcagctgtt gaagaactgg tggctaccgt agaagaagta
    18541 acgaatcagg taatggagaa caccaagtct accgatatcg ttcacgataa ggcaaaacag
    18601 gtaggtatcg aggcagccaa cagccagcag aagatggatg agctggtaga tgccatgaag
    18661 aaaatcgata ccacgaccca gaatatcgag aagatcatcg cagatatcga gaatattgca
    18721 tcccagacca atctgctgtc tttgaatgct tccattgagg cagcaagagc aggggaagcc
    18781 ggaagaggtt tcgcagtcgt agctgaccag atcagacagc tggcagaaga gagtgcaaat
    18841 tccgcagtag agtctaagaa gctgatcgaa gaatccatga acgaagtagg agtgggtaac
    18901 agggttacca gggagaccaa cgaagcaatg cagaaggtta tgaccgaact ggatcagatc
    18961 atccaggagg ttgctaatat ccgtaccgca tccgacagac aggcagtatc tgtcaacaac
    19021 atcaagaagg gtgtggagga catcaacgat gttatccagt ccaactcagc agcagcagag
    19081 gagacttccg ctaccagcga ggagctttcc gcttccgcta ctactttgaa cgagttgtta
    19141 gagcacttca aactgcgcgg ttaatcggag gatgttttcc caagatcgta atagagagaa
    19201 cagaagaacc atagctgaaa ccacagctgt ggttcttttc atatcggagg agtaatgagt
    19261 aataaggaaa tgcttcatag aaaacaatat aggatagcgg cggtagtggc cggtctggcg
    19321 cttgcagtaa gtatgacagc ctgtggtgca cagaaaggga ctgaggggac ggaaagcgtg
    19381 gaaactatgg aaaacgtgga tcctgtggtt cccgagagcg tagcagcatc cgtggagaca
    19441 atagaaaatg agaaaaacgc agaggaattc ggtagagaaa cgctggcgga aacgtctgtg
    19501 ccgcaagggg cggcgaatag tgcaggacat gaaatggtga cttcggggac cgacaaagtt
    19561 cccgggggag aggctgcggt gtcgcaaagc ggtatgccga cagaggccgc aggcagtcag
    19621 acagaaacgg tagccggtca gccggaggct atacagaacc agccggtgga atataccgac
    19681 ttacagcaga tcactctgaa tccggactgg gaatatgcgg atcattcgaa gataaatacc
    19741 ggagccgcag tactataccg ggcaccagag gagtccggaa gcaagggaat cattatcgga
    19801 gtgaatgcgg gacatggcac cgccggtggg gccaaagtaa agacgctgtg ccatccggac
    19861 ggttcggcca agactacggg aggcagtacg gcagcgggag ctacggaagc tgcggcagtg
    19921 tccggaggca tgacatttca ggacgggaca ccggagcgaa cggttacttt gcagatggca
    19981 cagattctgc gggacaaact gttggcctcc ggctatgatg tactgatgct cagggacgga
    20041 gaggatgtgc agttggacaa tgtggccagg accgtgatct gcaataacgt ggcagactgc
    20101 catatcgcac tgcactggga cagcggtgac ggcaagaatt acgacaaggg atgcttttac
    20161 atctctgtgc cggaagtact gaagagtatg gagccggtgg catcccactg gcagcagcac
    20221 gatgctctgg gagcggatct ggtggaaggg ctgcggggac agggagcaac gatctatgga
    20281 aaaggaaaca tgagcattga cctgacccag acttcttata gtacgatccc ttccgtggat
    20341 atggaactgg gaaatgcata ttccgatcac agcgatgcta tactggatca actggcggaa
    20401 ggattgctgc aggggattaa cgtttatttt caacagcagt aagattggac agagcggcaa
    20461 tgcattgatc ggagatgaac ttcaaaagaa atcccagcac tgccatcagc agcaattcaa
    20521 cgagaaacaa acctaaaaca cccaccatac cgcgcatata gggaatagcc ctcggagtga
    20581 taaaatagat aatgatatgg tggaccagaa atacttcata agagtagtta cttatcgtac
    20641 ggagaaatcg gccgtgtcct ttttcgagaa ccggttccag gcatgcagcg gaaatccaga
    20701 aggcaacggc cagtatcgtt attttcaaac cggtgctgat gggaagtgca aaagggcaca
    20761 gaacaaagaa aatcaccacc ggcagggata agaaaatcca tttaggattg aatttttcat
    20821 gataataacc aatcatcata ccgattacga actcgtagcc cttcaggaag aagttcatat
    20881 ggatggggac ggagaaatcg taatgaaata aaacaatcaa ataaatcccg gtagcgatga
    20941 taaaaaagaa cttttcactt tttatcataa aaaatcgaag caatggaaag atcagataca
    21001 acagaataag acatcccaga aaccattctc cgatggtaag ggaaaaggtg ctgatcccgt
    21061 gcatgctgac ccaggaatcc attccaagaa aagtaaaaac aattctccac gcaggaattc
    21121 cttcaggaaa aatattgtgg acggaatggc tctggaaaag gagaaacagg aaataaacaa
    21181 tgtaaagtat ataaaaggga atcaggatgc gcagaaatct tttcttataa tattttgcga
    21241 ggctgaagtt ctccttcgcg gtataggaga gactggcacc ggataacatg aaaaatacag
    21301 caacacccag ggttgccaga tgcatattgc tgttggaaaa cagaggattg agtctctcta
    21361 caggacagat tccggagaga tagagctgaa agagcagatg ataaaaaata atgaaacaaa
    21421 agcaggtaca tctaagcaaa tcaaaataac gtattctttt cattgatttt ccctctttgg
    21481 tttatgatat tgcatattat agcatatttt tatttcgaaa gaaaagtatt taggtgtttg
    21541 aaaaaaacac atattatgct ataattttag attatattgt tcaactaata aacagaaagg
    21601 caaaaattcc gtttatagag ggatttttga acgggtaaag ataaaatgaa gctgggtatc
    21661 gtaggtcttc ccaacgtggg aaagagcaca cttttcaatt ccctgaccaa ggcgggagca
    21721 gaatccgcca actatccctt ctgtaccatc gaccctaacg tgggtgtggt aacagttccg
    21781 gatgacagac tgaaagcact gggcgagatg tatcattcca agaaggtaac tcccgcagtg
    21841 atcgaattcg tggatatcgc aggtctggta aagggtgcct ccaagggtga gggactgggc
    21901 aaccagttct taagcaatat ccgtgaagtg gatgctatcg tacatgtggt aagatgtttc
    21961 gaggatccta atgtcatcca tgtggacggc agcgtgaacc ctctgcgtga tatcgagacc
    22021 atcaatctgg agctgatctt cgcagatctg gaagtactgg agagaagact gtccaaggta
    22081 gtcaaggcag ccagaatgga caagacctat gccaaggagc tggcactgct tgagcgtatc
    22141 aaggagcatc tggagagcgg taagatggcg aagagcctgg atgttaccga tgaggatgaa
    22201 ctggctctgt taaaggaata caatctgttg acttataagc ctgtgatcta tgcagccaat
    22261 gtagccgagg atgatctggc agatgacggt gcatccaatg agatggtggc aaaagtaaga
    22321 gagttcgcag ccggtgaaca cagtgaggta ttcgtgatct gtgcacagat cgaggaagag
    22381 atctccgagc tggatgatga tgagaaggca atgttcttag acgacctggg attaaaggaa
    22441 tccggtctgg agaaactgat taaggcaagt tatcatatct taggtctgat gagcttcctg
    22501 actgcaggag aggatgagac cagagcatgg accatcaaga ttggcaccaa ggcacctcag
    22561 gcagccggca agatccactc cgatttcgag agaggcttca tcaaggcaga agtagtaaac
    22621 tacaaggatc tgctggagca gggctctctg gccgctgccc gtgaaaaggg tctggtcggc
    22681 atggaaggca aggattatgt agtgcaggac ggcgatgtga tcctatttag attcaacgtg
    22741 taatactaag atccgcaata gacagattac gagactgctt gcaggcagag taatctgtct
    22801 attagcggat caaacataca tgagcaagta gggcgatagc ccggaatgcg aatgtatgtg
    22861 ccggattcgg gagcgcgaag cgcggagaag ccggatagta tggagccatg agcaagtagg
    22921 gcgatagccc ggaatgcgaa tgtatgtgcc ggattcggga gcgcgaagcg cgcagaagcc
    22981 ggatagtacc aagaaaggaa ataaatatta tgatgaatgc aggagatatt gcatttcccc
    23041 atttaggcat ttacctggag aatgttccca gaagcttttc cgtattcggt ttcgaaatcg
    23101 ccttttacgg cctgatcatc ggaatcgggg tactggcagg cattctgatg gcggtgcatc
    23161 aggcaaaggt gacaggagaa aatccggata cgatctggga tttcgccatt tatgccgtga
    23221 tcttttccgt gatcggagcc agaatctatt acgtggtctt tgcctgggac tattacaagg
    23281 acaatctgtt aagcatcttc aatacccgta acggcggtat ggcgatctac ggcggtgtga
    23341 tcggagcatt cctgacactg ctcatttatt gcaggatcaa gaagatcaac ccgttccggc
    23401 tgggagatct gtgtgtgcca gggttgatcc tgggacagat catcggccgc tggggtaact
    23461 ttatgaaccg tgaagtattc ggggaatata cggacagttt tctggccatg cggcttccta
    23521 tcgaagctgt gcgcagccgg gatatttctg agaatgtagc agctcacatt atcgatggaa
    23581 ccaattacat tcaggtacat cccactttcc tctacgagag cctgtggaat ctgatgatcc
    23641 tgatccttat gctggtgtac cgcaagcaca aaaagttcga gggtgagatc tgtctgctgt
    23701 acttcggagg ctatggtctt ggcagattct ggatcgaagg tatccggaca gataccctgt
    23761 ttatccccgg aacgaccctc gccgtatcac aggtattatc cttgtgcatg gtgatcttcg
    23821 cagtggtgac ggacgttgtg gtacgtatcc gcctggaaaa acagccgaaa gtggaaaaat
    23881 attaaaaatt tttaaaaatt tattaagaat atcgaaacag gagcaaatac atagatattg
    23941 tgtttgctct tatttttata ccagatattg tagcaaaaag cgcctttgtc gtacgacagg
    24001 gcgttttttt gcgtggtgct gtcgaaaaaa gctacttgat ttctcttgta aaatacccta
    24061 ttttagtata aaatcataat aaaagtggga gaaagtggag gtaagtgcca caaagtggta
    24121 gatttttaga aaaatgacct ccttttcgtg gcagtcccct tttggatgaa tggtggtgag
    24181 tgcgatgttt atgagcgagt acaaccatac gttagatacg aaaggcaggt tgatcattcc
    24241 ggcgaaattc cgagaagtct taggagagga atttgtgatt tccaagggaa tggatggctg
    24301 cttatttgtg tatgccaacg atgactggaa tgcttttgaa cagaagctga cctcattacc
    24361 tctgatcaat aaagaagcaa gacaatttgc ccgtttcttc ctggccggtg cagctacggt
    24421 agaagtggac aagcagggaa gaatcctgtt gccggcagca ctcagagagt tcgctggact
    24481 ggaaaaggac gtggtactgg tcggcgtcgg aagccgtgtg gagatctgga gcaaggataa
    24541 atgggagaat atgaactccg ataccgatat ggatgagatc accagcgcca tggaaggact
    24601 gggactgacc atttaacggg tgaaaaacgt gaccgagaaa ggatgaccgt cgtggaattc
    24661 aatcattatt ctgtgatgct gcatgagacc attgaacaac tgaatattaa cccggacggc
    24721 atctatgtgg acggaacttt aggtggagga ggacatgcct acgaagtatg cagccgcctg
    24781 aaaaacggac atttctacgg cattgatcag gatgatgcag caatcgcagc agcatccgca
    24841 agattgcagc cgtttggaga taaagtgacc gtgatccgca acaattatgt gaatgcagtg
    24901 gaggccctca gagaatatgg tgtcaccggc gtggacggaa tcgtgctgga tctgggagta
    24961 tcttcctatc agctggatac cgaagaccga ggattttcct atcggtttga tgcccctctt
    25021 gacatgcgga tggatcgcag gcagaccctg acagcgagag atatcgtcaa caattattcc
    25081 gagatggaac tgtatcacat catccgggat tacggtgaag atccttttgc caagaatata
    25141 gcgaaacaca ttgtgaaaaa cagggcagac aaacccattg agaccacctt tgaattaaat
    25201 gagatcatca aagccgcagt cccggcaaag atgcgacaga acggacatcc ttcgaagcag
    25261 actttccagg caatccgtat cgaatgtaat caggaactgg aagtactgaa aaaggcgctg
    25321 gacgaactga tcgatctgtt gaatcccggc ggaaggttct gcattattac cttccattcc
    25381 ctggaggacc ggatcgtgaa gaacgcattc cgcaggaatg agaatccctg cacctgtccg
    25441 ccggaatttc cggtgtgcgt atgcggtaag gtctccaagg gaaaggtgat caccagaaaa
    25501 cccattcttc ccagtgtgga agaactggaa gtaaacagca gatccaagag tgcaaagctc
    25561 agggtatttg agaaaaaata aaagacttta gtagtgaaag cggggagaaa gagtatggca
    25621 cagagaagaa gagtaagcaa tactccttac atatatgata atacagcaag agacttacag
    25681 atccgcaggc agatggagga gcctcggaga cagatgtctc atgagacgag aaaaaacaga
    25741 gaaaaagcaa gacatatgag catcggctac gtcacctttc tgatgggagc cctgattgcc
    25801 tgtgccttct gcctggtaaa ttatatccag atgcagtcgg agctgaccac caagatcact
    25861 gaggtgtcca gaatggagag tgaactgaac tctctgaagc tgtcaaacga tgaagagtac
    25921 agccggatta ccagcaatat caatctggaa gaagtcaaga gaattgccat cggcgaactt
    25981 ggaatgacct atgctgccga aggacaggta atcacctacg caagcagcag caacgactat
    26041 atgcgtcagg cggataccag ccagaactaa gtctgggagg cagaaagcgt gaaggaacag
    26101 aagcctaaga aattttccat aaagatgcag aaaaaacttt tatgcctgta tgccctgatc
    26161 atactggcat ttattctgct gtctgtccga ctgtcctgga tcgtgaaagc ggatggagca
    26221 acctacgaaa aacaggtttt gtcacagcag aaatacgact ccgtgacatt gccgttccgc
    26281 cgcggagaca tcgtggatac caagggaaca aaactggcag tcagcgagaa ggtatataat
    26341 ctggtcattg attcctatgt aatgttaagc cgtgagactt atctggaacc gacgatggag
    26401 gcgctgtccg caaatttccc ggatctggat atggcggcag tgagacagta tgtgatcagt
    26461 catccggatt cttcctggta tgtccctctc agaaggctga cctatgatca gatcagcggt
    26521 tttcaggcgg cagcactgga ggacagcaag atcaagggcg tctggttcca ggaagaatac
    26581 aagagagtct atcccaatgg ttccctggcg gcggacgtga tcggattctc cagatcggac
    26641 aacgaaggac agtacggact ggaagagtat tacaatgatg tgctgaacgg aaccaccgga
    26701 agagagtatg gatatctgaa tgatgatgcc aacctggagc ggaccatcaa accggccgtg
    26761 gacggcaata cgatacacag taccattgat gccaatatcc agggtatcgt ggaaaaatat
    26821 ctgaaacaat tcaacgatga gcataaggat gcggtacatc ccggtaacgg tgcggaaaat
    26881 gtgggctgca ttattatgga ggtaaatacc ggcaacattc tggccatggc cagctatccg
    26941 acctatgacc tgaacgacac caggaatacg gacgcccttt tgggatcccg taagatcgag
    27001 atggtgacca atgccaacgg ttatgatgtt ctgaccaaga cagataccta ttttacgcag
    27061 gaagacattg atgccttaac ggatgacgaa ttgctggaca acctgaatta cctgtggaag
    27121 aactattgta tctccactac ttacgagccg ggttccacag caaaaccgtt tactgtggcg
    27181 gcagcactgg agagcggtgc tatcaccggc aacgagcact atcagtgtaa cggatctctg
    27241 gaagtcggcg gacatacgat caagtgtcac agctatcgtt ccggcggtga aggagatgta
    27301 tccgtacagg attccattgc ctggtcttgc aacgtggcgc tgatgaagat tgcagctact
    27361 ttgggaaaag aagagtttgc caaatttcag caaaccttta actttggatt aaagactaac
    27421 gtggatctgg caggagaagc gagaacggca agcctcctgt tcccggtgga tcagatgggc
    27481 agtgccgatc tggctaccaa cagtttcgga cagaacttca atgtaacaat gatccagatg
    27541 atcacgggat tctgttccct gatcaacggc ggttattatt atgagccgca tatggtggat
    27601 aagatcacca attctaacgg cgcaacagtg gaaaacatcg agcccagagt actgcgtcag
    27661 accatttccg agagcacctc ggcgaagatc agacagtact gccgggctac cgtaatggaa
    27721 gagggtggtg accgcagaac cggtagaacc gcaagacctg caggttatgc catcggtggc
    27781 aagaccggta ccgcagagac cattccccgt aaaaacggtg agtacgtagt atcctttatg
    27841 ggatatgcac cggcggatga tccccagatc gctatctatg tggtcgtgga tcgtgccaat
    27901 gcatctccgc aggatgatgc gaagttcgct actggaatcg taagaaatgt actgacagaa
    27961 gtgctgcctt acctgaatat tttcatgaca gaagaactgt ctgagaagga gatccaggag
    28021 ctggcagaaa aacagatcga gatcaccaat cagtataccc agaagcccga ggacggtgaa
    28081 gatcagaata acggtgacgg aaccggtgat ggacagaccg gagacgatca gacgggcagt
    28141 ggagatggaa ccggtgacgc tcagggcggt gataatacaa cgccttccga ggattggaga
    28201 aattatccgg tggatccggc caccggttat ctggtaagcc cgattgacgg tggactgatc
    28261 gaccctgtta cgggagcgga tgtcggcggc gatgattccc tgggggacag cccggtcaac
    28321 aataatctgc tgaaccagca gaatgaatag taaggtaatc tgacgaatag cctcacagca
    28381 agagcaataa aatgtaaaaa gctcttgttg tgaggttttt ttatggcaga tcatcagaat
    28441 cgtttctttc atcggaagaa gatcatgacc atatttttcg tatgtgcggc agtgctctgc
    28501 ggactgttgg gaagactatt gtatctgatg gtatggcggg cggaatatta tgaggaagcg
    28561 gccacaaggc tgcacgaaag agagcgcagc atcaaggcgg cgagaggacg gattctggat
    28621 cgcaacgggg tcgtgctggc ggacaacaag atggtctgta cggtatcggt gatccacaat
    28681 cagatcgagg agccggagca ggtgatcgcc atactggtga aggaactggg aatctccgag
    28741 gaagaggcca gaaagcgggt ggagaaatat tcctccatcg agcgggtgaa aagcaacgtg
    28801 gataagaccg tgggagaccg gatccgggaa tatgatctgg ccggggtaaa agtggacgaa
    28861 gattacaaga gatattatcc ttacggggat ctggccagca aggtgctggg ttttaccgga
    28921 ggagacaatc agggaatcat cggactggaa gtggtatatg aagagatcct gcagggggat
    28981 ccgggaatga tcctgacgat cacggacgcc aaggggatcg aggtggatac ggcaggggag
    29041 cgtcgggtgg aaccggtgcc gggcatggat cttcgtatca gcatcgaccg gaacatccag
    29101 gagtatgcta cacagctggc ggcgcaggcc tgtgccacca aggaggcgga cagtgtttat
    29161 atagtggcta tgaatccgca gaacggcgag atcctggcta tggtgaatta tccggagttt
    29221 gatttgaatg acccttatac cttaccggag ctgacggaag tgaaggagga cggcagcgtt
    29281 gtggcggtgg acccggacac actgacggcg gaacagaaac aggaaaagct gaatgccatg
    29341 tggcgaaacg gctgtatcaa cgacacctac gaaccgggat caaccttcaa gatcatcacg
    29401 gccgctgcgg gactggaggc gggagtggtg accccggaaa gcaccttttc ctgccccgga
    29461 tttatcatcg tggatgaccg taagatccgc tgccacaaga ttgccggaca tggcggccag
    29521 acttttgtac agtgcatgga gaacagttgt aacccggcat tgatttccgt cggcctgcgg
    29581 ctcggtgtgg acaattacta ccattatttt gaacagttcg gcctaaaaca gcgcaccggc
    29641 atcgacctgc cgggagaagc tgggactatc atgcataaaa aagaggatat gggaaatgtg
    29701 gagctggcta ccgtggcttt cgggcagtct ttccagatca cagcaatcca gctgctgacc
    29761 acggcttcct ccattatcaa cggcggcaac cggatcaccc cgcatttcgg catcgaggcg
    29821 ctgaaccgtg acggggaggt ggaagagacc ttttcctatc cggtgcagac ggggatcgtc
    29881 tcggaggaga cttcggctac tatgcgctac attctggaaa tggtagtggc ggaaggatcc
    29941 ggcagaaacg gtcaggtaca gggcttccgc gtcggcggca agactgcaac cagccagacc
    30001 ctgcccagag gaagcggacg ttacatatct tcctttatcg gcttcgctcc ggcggatgat
    30061 ccccaggtca tcgccattgc tattatcaat aatccccagg gcatctacta cggggggcag
    30121 gtggcagcac cgatcataag gcagctgtac gaaaatattc tgccctatct gggactggag
    30181 aagaccgaag agccggagga gacagcggag cccggggagt gatggcggtc gcagacttcc
    30241 ccagggacga tgtcgaaaac gatggaaaga aaaaggttcc ggcggatgaa aatttgacaa
    30301 aatccgctga tgccaccctt tataatgttt ctgaaggagg ggtgtgatgc attatgaact
    30361 ctacttagac agtctgttcc tcctgaattt cgggatgaat ctgctgctgc tttgcctggt
    30421 ggatcacagc atgggccaca ctgccacgtg gtaccggctg cttgcgggag ccgtggtcgg
    30481 agcggtctgg tatctgtttc cttttttatg gaaaggaaca gccgccggga agctgttgct
    30541 ttgttttctg ccggggacaa tacttatgct gtatgtgaca tttcacattc gcagccttgg
    30601 gacgttgtgg aactatttcc gaaaactgat gctgtccacc ttcctgatcg gaggcattct
    30661 ggcggcactg cttcggggat tcccgatggg aagaaattat ctgcctggga tcggtatgac
    30721 cctgggagtc ggagcattct ccggctggat acttgtgcgg gggcgcaaaa aggaagcgga
    30781 acaagcctgc tattgtgagg tagtgttaca gggagcggag ggaccgttgc gcattcatgc
    30841 attggtagac tccggcaata ccctgaccga acctatcagc ggagcaccgg tatcggtgct
    30901 ggacgcacgg atattttatc ggctgtggcc ggagggactg cctggattcc gggtgattcc
    30961 ttatcacagt gtagggaaaa agagcgggat ccttaaggga tatccggtgc cgcagatgac
    31021 gctcatacag cagggtgtgg aacgagaata cagaaatgta tacttggcag tgagtgagga
    31081 gatcgcggga gaagagatcc cgatgctgtt acctcctgcg ctgttgcagg agacgaacaa
    31141 agcacggagg aagaaggaaa aaataaaaga gccggacaat ccggcaaggc acaatgtggc
    31201 agataggagt aaatatggtc ttaaagatag caataccggg aaaaatgcag tttaaaatga
    31261 tgcgcaggga gccttatctg ctgcaccgga aaggagatgt ccactatatc ggtggcgcgg
    31321 aggtactgcc gccgcccctg cccagtgagc aggagaatca ggtgatcggt gatctgggga
    31381 cggaatacga ggatgaggcg aaagccatcc tgatcgaaca taacctgcgt ctggtggtct
    31441 acatagccaa aaaatttgac aataccggtg tgggggtgga ggatctgatc tccatcggca
    31501 ccatcgggct gatcaagtcc atcaacacct tcaacccgga gaaaaatatc aagctggcaa
    31561 cctatgcctc ccggtgcatt gaaaatgaga tactgatgta tctgcgccgg aataacaaga
    31621 cgaaaatgga agtctcgatt gacgagccgt tgaatgtaga ctgggacggc aacgagctgc
    31681 tgttgtcgga tattctgggg acggatgagg atgtgatcta tcgcggtata gagaatgagg
    31741 tggagcggaa acttctcatg aatgccgtga gcaagctgtc caagcgggag aagaccatcg
    31801 tcagacttcg gttcggactg gggacagtgg acggacagga gatgacccag aaggaagtgg
    31861 ccaatctgtt gggaatctca caatcctata tttcccgact ggagaaaaag atcatgcggc
    31921 agctgaaaaa ggagatcagc ctgcatattt gactttgcat tcgggacgga tacatgatac
    31981 tatataacat gtgcagaatt gtgggagctg cgtggcaaca gagcagttcg tgaaccatat
    32041 aatacagaca ggatagtttt caggtaacgg aatcggaagt aaccaaaaag gacaaataca
    32101 tgatacagcg tgatgatata ttatcaatgg aatatttaaa aaagacggaa ttcaccggat
    32161 gccaccaggg gatgcgttac cgtctggagc agactgagga tccggaaggc ggcaaaaagc
    32221 ttctcgtgac ggtctggccg gagcccttta actttttaaa gacaccggag gagaagaagc
    32281 agagggctat gttctccttt gacgaggacg gtgtggtgga cgcggttggc tggatgaacg
    32341 acagattatt tgagaataag aagctatggg atgaggcccc ttctcactgg gacacttatc
    32401 agatgtgaaa cgggaatttt aagagacaga tataggatga agaaagaatg atttggaaat
    32461 atataataag ggatctgaac ggtgccatac aatatctgcc ctatggtgtg atggccggtg
    32521 ccgtcatggg actgatcctg aatgcaatga atgccaggag ggagcggaag ggaaaagaag
    32581 cctttccgat ggcaggtctc atggtgtttt ctttgtacct tatgatcata ctggtgatca
    32641 cttttttctc cagagaggat ggcagccgga accgtgccat ggatcttgag cttttctcca
    32701 cctgggggat caatacgaga aataacgcct tcgtggtgga aaacgtgctg cttttcatcc
    32761 cgtatggctt tgcatgcccc tgggcatttc cctggcttag gggatttttc aggaacatct
    32821 ttgtggcttt tgtgaccagc ctgggagtgg agactttcca gctcattacc ggcagaggtt
    32881 attttcagat cgatgatatc ctgaccaacg tgctgggagg aatcatcgga tactggatgt
    32941 tttggctggt gtatcatacg ttacagagaa tacgaggatc aaggtctatg cggtaagata
    33001 gcctcgcata gctttcaatg catttttttc gagacgggag acctgggcct gggagatgcc
    33061 gatcatgtcg gcgacctcgg tctgggtctt tccttcgaaa aagcgcagag agatgatatg
    33121 acgctctctg tcgttcagac gtttcatggc ttcactgagg gagagatgtt ccacccacgt
    33181 ctcttcctta ttttttttgt cactgatctg gtccatgacg tagagggtgt caccaccgtc
    33241 ggtgtagacc ggttcgtaga ggctcatggg attctgcatg gcatccaggg catagacgat
    33301 gtcctccttg gagataccta cttctgcggc aatctcttcc atggtgggct ctttcagatt
    33361 tttcctggtc agggtatcgc gggcgtagat tgccttgtag gcggtgtcct tcagagaccg
    33421 tgaaacacgg atactattgt tatcccgtaa aaatctgcgt atctcaccga taatcattgg
    33481 aaccgcgtag gtggaaaatt ttacacccag cgtggaatca aaattatcga tggccttgat
    33541 cagaccgata cagccgatct ggaacagatc gtccacattt tccgcactgc cggaaaagcg
    33601 tttgatcacg ctcaggacca gacgcaggtt gccttcaatg taggtctctc tggcggcggt
    33661 atctccctcc cggatctttg cgaagagagc ttccttgtcc gctgccttta gcaaaggcag
    33721 ttttgctgta ttgacaccgc agatttctac ttttccctgt accatagaga cctcccaagt
    33781 tttctgaatc gttgctgatt tttgataact acagtattca caggagagtc agtgactata
    33841 ccggcaagaa agattttcca gtttttcccg aacgggaaac ggagcggtat catataataa
    33901 atagaaagaa aacgggagga gcagagtatg cttttttccg agttaaaatg caaagatgta
    33961 atcaatgtca gagactgtaa aaagctgggg catgtatgtg atctggagtt cgatgagtgc
    34021 agcggatgca tctgtaagat catggtgccg ggatgcaacc agtggctttc atttctgcgc
    34081 tgtgagccga atatcgtcat cccgtataag gacatcagac agattggacc ggacataatt
    34141 ttagttgaca ttaactgctg aatcttctat aatcttaaca taaataacac gtggttttta
    34201 taaggaggat gcctatgaaa tgtccgttct gtagccatga aaataccaga gtcatcgatt
    34261 cgagaccggc ggaagataac aactccattc gccgccgtcg tgtatgtgat gaatgcggca
    34321 agcgattcac cacttacgaa aagatcgaaa cgattccgct gatcattatt aaaaaggaca
    34381 ataacagaga ggcctatgac cgttctaaga tcgaggcagg agtactcaga gcctgccata
    34441 aacgtccggt cagcgcacag cagatcacga ctttggtgga tgaggtggag aacgagattt
    34501 tcaacaggga agaacgggag atccccagcg gcaccatcgg tgagcttgtg atgaataagt
    34561 taaaggatct ggatgcggtc gcttatgtga gatttgcttc ggtctaccga gaattcaagg
    34621 atgtgaatac cttcatggat gagctgaaga gtgttctcaa cgataagaat cacaattaaa
    34681 atcagaacag gacttctgta tttacggaat agcacacctc cgtctgcccg gcataagttg
    34741 gacagggagg tatttttatg cgataaattc agaaaactgt gttttggcgg aagaaacggt
    34801 acagacagaa aaaatagctt ccggccagca ccagctgact tgccagaatt ccccacagat
    34861 atccctgcat gccgatctgt ggaatggcga aaaatacaaa ggccagacgg atcagcagac
    34921 agaccacatt ggagaaaaag atccgccccg ccattcccag cccctgcaaa atgctagaga
    34981 gcgtggtatc cagatacaaa aaaggacaga tgaatcccag cgtcacgatg aaatgcccgg
    35041 ccagttcact gtggaacaat tcggttccca tccaatcccc cagaaaaaga aaaacgcaca
    35101 gacaaagaaa gcccatgaga ctgcagtatt ttacggtacg cagagtgtcg ttccatacgg
    35161 cttcccggtc accttttgca tgattttcgg agatggtggg gaggagaagg accgccacgg
    35221 aaccggtgag agcattggga aaaaagatca tgggcatggc cataccggtg agtacgccgt
    35281 atacacttaa ggctgtggtg gtatcataac cgtagagcag aagcttctgg ggaatggaga
    35341 cagcttcgat gctctgcaaa atattcaaaa cgatacgatt ggcggtcagg ggcagtgcca
    35401 tgcccaacag atcccggtag accgcggaag aaggacagat attttgcagg gaaagaacag
    35461 aggcatccgg atacaggtgt gtgtacaggc agatcagagc gatcagcatg gacagaagct
    35521 cgccgacggt cagacctaag acggccgcat tgatggaggg ggtgcgcccc tcagacagat
    35581 cggctccggt gacgagaaag acacagccga ctctggccag ctgctccaga agctgcgtga
    35641 gggcaggaac ggaagctttc ttttttccat agaaatagcc gttgatgcag gcatgcagcg
    35701 cggcaaaagg aatagagaag gacagaatcc gcagcatgga tatggtacgg gcttctttta
    35761 acaggaaggt tcctatgggt tctgcggcga gaaacaggac agcattgact ccaagggaca
    35821 gagggagcgt gatgcttgcc ccggtcagca gcgttttcag gtcatcggaa cgccgccccg
    35881 cagtattggc ggcaacgaat ttggagatgg cggtctggta gccggcagct gtcagggaaa
    35941 aggagagaga cagtgcggga ctgagcagct gatagatccc cattccttct tctccgaaga
    36001 gcctggacag gtagatccgg tagaaaaaac cgatgatgcg ggtgataaga ccggtgatcg
    36061 tcagaatcag agtaccgaag atcagaggat gacgtttggc atgcttcata gcttttcgtt
    36121 ggatcctttg cagataaaga tcttgcaaaa gtatattcca tccaccttgt tgacagaact
    36181 gcgcggtgaa tgctatcatg aataacagtg aaataagcat gaaaatatgc ggaaatgagg
    36241 caatatgata tatttagata atgcagcaac taccaaaacg gcaccggagg tcgtggatgc
    36301 gatgcttccg tacttcagtg aatactacgg caatgccagc accatctaca gtctgggtgc
    36361 cgagagcaag aaggccatgg atcatgcccg ccagaccatc gcagacagtc tgggtgcaaa
    36421 gccggaagag atctatttta ctgccggcgg ttccgagtcc gataactggg cgctgaaagc
    36481 taccgcagaa gcctatgctt ccaagggaaa acatatcatt accacgaaga tcgagcatca
    36541 tgccattctt catacctgtg agtatctgga gaagcgaggc tttgagatca cttatctgaa
    36601 tgtggacaga gacggactga tcagtctgga tgagctgaag gcagccattc gccctgacac
    36661 gattctgatc agtgtaatgt ttgcaaataa cgagatcggt accatcgaac ccattgccga
    36721 gatcggtgag atcgcgaagg agcacggtgt attgttccat accgatgcgg tacaggcgta
    36781 tgcacaggtt cccatcaatg tggatgaaat gcacatcgac atgctcagtg ccagcggcca
    36841 caaattaaac ggccccaagg gtatcggctt cttatatatc cgcaagggtg tgaagatccg
    36901 ttcctttgta catggcggag cacaggagag aagccgcaga gccggtacag agaacattcc
    36961 tggtatcgtg ggactgggtg ctgctgtaga gcgcgctatg cgtattatgg ataccaagac
    37021 ccgcaaggag atcgaactgc gggattatct gatcggacgt ctggagaacg agatccctca
    37081 ctgctggctg aacggtcacc gcacgaagag acttccgaac aatatcaact tctccttcct
    37141 gttcatcgaa ggagaatcca tgttgatcat gctggatatg aagggcattt gcgcttccag
    37201 cggttccgca tgtacttccg gatctctgga tccttcccat gtactgctgg ctatcggctt
    37261 aaagcatgag gaagcacacg gctccttgcg tctgacctta tctgaggaaa gcacgaagga
    37321 agagatggac atcgtggcgg aagaagtgaa gaaaatcgta cagagactgc gggatatgtc
    37381 tcctttatat gaagatttcc tgaaaagcca gaagaacaac taagtgagaa agaagaggat
    37441 aacagataca tgtacagtga aaaagtaatg gaccactttc agaaccccag aaatgtaggg
    37501 gagatcgagg atgccagcgg cgtaggtacc gtgggcaatg caaagtgcgg agatattatg
    37561 agaatgttcc tggacattga cgagaacggg atcatccggg aagcaaaatt caagaccttc
    37621 ggctgcggcg ctgccgtagc taccagctcc atggctaccg aactggtaaa gggtaagacc
    37681 attcaggaag cattacaggt aacgaacaag gccgtaatgg aggctctgga cggactgccg
    37741 cccgtaaagg tacactgttc actgcttgca gaggaagcga ttcatgcagc actgtgggat
    37801 tatgcagaga agaaccatat caccatcgaa ggactgcaga agcccaagaa tgacatcagt
    37861 gagggcgaag aagaggaaga ttattaagat ctatgaagaa aaaagtagta gtaggcatgt
    37921 ccgggggagt agattcctcc gtggcagcca tgttattgaa ggaacagggc tatgacgtga
    37981 tcggcgtcac tatgcagatc tggcaggacg aagaggaagc agccaaggaa gccaacggcg
    38041 gctgttgcgg actgtcggcg gtggatgatg ccagaagagt ggcacagaga ctggagattc
    38101 cttattatgt catgaacttt aagaaggaat tcaaatgcca tgtcatggac tattttgtgg
    38161 cagagtattt aagaggccat acccccaatc cctgtattgc ctgcaatcgt tacgtgaaat
    38221 gggaatccct gttacagagg agcttagaga tcggtgcgga ttatattgcc acgggacatt
    38281 atgcgaggat tgacaggctg ccgaatggcc gttttgccat ccgtaattcc gttaccgcag
    38341 ccaaggatca gacctatgcg ttgtacaatc tgacccagga gcagctggca cataccctga
    38401 tgccggtggg tgaatatacc aaggatgaaa tccgcgtcct ggcagaggag gcgggacttc
    38461 cggtggcgca taagccggac agtcaggaga tctgtttcgt gccggacaat gattatgctt
    38521 cctttattga cagagaagcc ggcgaaaagg tgccgggacc gggcaatttt gtcacggagg
    38581 acggcaggat cttaggccgg cataagggca ttacccatta tacactggga cagcgcagag
    38641 gactggaact tcctatgggg gaaagagtgt ttgtcaccgc catccgtccc gagaccaatg
    38701 aagtcgtcat cggttccaat caggaactct ttacggataa agtgctgtgc gatcatgtga
    38761 attacatgac agtggaagat ctgacagagc ccaccagagt attggcgaag atccgttaca
    38821 accataaggg agaatacgga actctgacaa aacagccgga cggtaaagtg ctctgtacct
    38881 ttgatgcacc ggtacgtgcc gcaacaccgg gacaggccat ggtattttat caggatgagc
    38941 atgtactggg cggcggaacc attattctgt aggaaagcgc tcaaagagga ggtctctgcg
    39001 tcctacggat acaaagattt gtatagcgga aaagatcggg atcactattg ttacaatagt
    39061 ggtcctgcat ttttatcacg gcgtatgaca acagtggcac cgacgtgcat ataatagtag
    39121 aaagctttta gcaacagcag gagtgtttat ggattatcgt ttttcatttc agagacctat
    39181 gagaagaagc agtttcggct ccatgaaggg aaccattgtg gatctggtac cggcaggaca
    39241 gggtggccgc agagccgatg gatgcctttt gttcgctaca atcgaggata cggacggcaa
    39301 cacggtgaac tttttcatca acccggatac gtatgtggtg gatttcacta cactgtcggt
    39361 gggaatgatg gcaaccttct ggtatcgcaa tgatgcaccc atgcccctga tctatccgcc
    39421 tcagtatacc gcagtggtag tagcggagga ccggaatgac cggtatgtgg atgtgagcta
    39481 ctacagcaat tccctggtca atgaggagca gacattgcag ctgaatctgg atggcacagt
    39541 ggatgtgcgt accgtgaaca accagtattt ccaggcgagt cccgcgaacc ataatctggt
    39601 ggtcatctat tcctcttcca cgagaagcat tcctgcccag accacgccgt ctacagtggt
    39661 ggtactgtgt gacagaaact gtggttaggc agacaaaaga ggacagttgt agtccattga
    39721 cctattgaga tttttgcaaa aatgttatac cataaaacaa tcccctatag aatgatgtcg
    39781 ggggcaaaag tccccgactt tgatgcctac ggattgttcc gtgctgcgca ctcccacaat
    39841 ccgccccaat attcgcatat cgtgggatta tcatcccact tttatgctca tatcggggcg
    39901 ggtcccaagg tctttcgc
//
LOCUS       DAWEJW010000011        39314 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_463_length_39314_cov_57.666752, whole genome shotgun sequence.
ACCESSION   DAWEJW010000011 DAWEJW010000000
VERSION     DAWEJW010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 39314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 39314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..39314
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_463_length_39314_cov_57.666752"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT        11323 a         7903 c        10523 g         9565 t
ORIGIN      
        1 aaaccgaagc ttttctccgt gatgaaaagc tacacgaaag aacagtttgt aaaagatgtg
       61 atcgcaggta tcatcgtggc gatcattgcc ctgcccttat ccattgcact ggcactggct
      121 tccggagtaa caccggaaaa gggtatctat acagcaatca ccgcaggctt tgtgatctct
      181 ttcctgggtg gcagccgcgt acagatcgca ggtcctactg cggcatttgc cactatcgta
      241 gccggtattg tggcgaagaa cggcatggac ggtctgatca tcgcgacatt gttagcgggt
      301 gtgatcctga tactgatggg attattccga ttgggtaatc tgatcaagtt catcccttat
      361 accattacca cgggctttac ctcaggtatt gccgtgacaa ttctgatcgg acagatcaag
      421 gacttcctgg gactgaccat tgtcagtgaa gagccgctga tcgagaccat ggacaaatta
      481 aaagcggtat ttcagtttat atccaccacc aatctgcagg cccttctggt gggtgtgatc
      541 agtctgctga tcctgatcgt atggcagtat ctgccgggct tctgcaagaa gattccccct
      601 tcactgattg cagtactggt gggaagtgcc atggtagcac tgcttaatat gaatgtaaac
      661 accatcggag atctgtatga gatctccaat aagctcccga caatttctct gccgtccttc
      721 agcctgaaga ccgtacagaa tgtcctgccg gatgctttca ccatcgctat tctggcggct
      781 atcgagtctc tgctttcctg tgtggtggca gacagcatgg tgaatagtag acaccgctcc
      841 aacatggaat tgatcgccca gggtgccggc aacattactt ccgcattgtt cggaggaatt
      901 ccggctaccg gagccattgc cagaaccgca gccaacgtga aaaacggcgg acgtaccccc
      961 attgcaggta tggtacattc tgtgacactg ctgttaatac tggtggtgct gatgccttat
     1021 gcggcactga tccccatgcc taccatcgca gcgatcctgt tcatggtggc atacaatatg
     1081 tgtgactggc ggaagttcgt aggactctgc aagactgcac ccaagagcga tatcatcgtt
     1141 ctggtgacca cgtttgtact gaccgttgtg tttgatctgg tggtagccat tgaggtaggt
     1201 atcctgctgg cagccattct gttcatgaaa cgtatgagcg atgtgacgga agtggaaggc
     1261 tggaaatatg tggacgatga ggatgatgcg gacagcctgt ccttaagagt cgtaccgcac
     1321 aacactatgg tttatgaggt cagcggccct ctgttcttcg gcgcagcgga caagatcctc
     1381 aagatcacgc tggatgagaa gatgaattgt ctggtactgc gtatgcgcag cgtgagcgct
     1441 atcgatgcca cagctatgca taatctggaa cagctatata cagactgcaa gaagaaaaat
     1501 atccagatca ttctgtccca tgtaggggaa cagcccatgc acgtcatgga gaagagcggt
     1561 tttctggaca aggtcggcag agagaatgtg tgtgcacata ttgatgatgc actggagcgc
     1621 gccgcacggt tgggataagg ggcaaaaact atcatttgtg gacgggttct ttaatttaca
     1681 acctcaatat tttgtgctaa gatagatgcg gatacgcaag cttttgcgtg tccgctttgt
     1741 cgtgtattct tttacagaac aggagaaaat catggctgaa ttgacaccaa tgatgcaaaa
     1801 atatatggag accaaggagc agtataagga ctgcatcctt ttttatcgcc tgggagactt
     1861 ttatgaaatg ttctttgacg atgctatagt agcatccaaa gagctggaaa ttgcactgac
     1921 cggaaaaagc tgtggactgg aggaacgtgc tcctatgtgt ggcattccct atcatgcggt
     1981 ggagacatat ctggccagac tggtcagcag gggctataaa gtagccatct gcgaacaggt
     2041 ggaggaccca aagctggcaa agggactggt gaagagagag gttatccgtg tggtgacccc
     2101 ggggaccaac ctggatgtcc agtcgttgga agccagcaag aacaattacc tgatgtgcat
     2161 tgcctatacc tcggatggaa tcggcatttc cgcagcggat gtcactaccg gagattatta
     2221 tgtgacagag gtagaagacc tgcggaaact gaaggacgaa ctgatgaaat atgagccctc
     2281 ggagatcatc tgcaatgagg cctttctggt cagcggatat gatgtggagg acttgaagtc
     2341 ccggttacat atgtcggtca gctcactgga gtctcacatg ttcgatgatg acggctgccg
     2401 gcggatcctt atgagacatt ttaaggtgaa tactctgatc ggcctcggtg ttgaggaatt
     2461 tcctacagga attctggcag caggagcatt actgcaatat ttatatgata cccagaagac
     2521 ggatctggaa cattttactc atatttctcc ctatcttacc agcaaataca tgctgttgga
     2581 cagttccacc agaagaaatc tggaactgac agagacactg cgggaaaagc agaagagagg
     2641 gtctctgttg tgggtactgg acaagacgaa gacggccatg ggcggcagat tgctgcgaaa
     2701 ctatattgag cagcctctga ttgataagga agaaatggag aaacgtctgg atgcaatcca
     2761 ggaactgaat caggattcca tctcccggga cgagatcaga gagtacctga atccggttta
     2821 cgatctggaa cgactgctca gcaaagtaac ttataagacg gcaaacccca gagatctgat
     2881 cgcttttcgt aactctctgc agatgctgcc gcctatcaag acggtgttgg cgggcttcca
     2941 gaaagaggag ctggcagcca tccgggaaga aatcgatgga ctggaggaca tttatcagct
     3001 gattgacgag gcgatcgttg aggaaccgcc catatctatt cgtgaaggcg gaatgatcaa
     3061 ggatcagttc gatgagacta tagatcatct gcgtgcagcc aaacatgacg gcaaacaatg
     3121 gctggtccag ctggaagaag aggacagaga acgtaccggg atcaagaatc tcaaaatcaa
     3181 aaaaaataat gtgttcggct acttctttga ggtgaccaat tcctacaagg atctggtgcc
     3241 ggaggattat atccgcaagc agacccttgc caatgcggag cggtatacca caccgagatt
     3301 aaaagaactg gaagatacga tcttaaatgc ggaggacaag cttcagacac tggagtatga
     3361 tattttctgc agaatccggg ataccatcgc acaggaactg gtacgtatcc agaatactgc
     3421 caaggcgctt gcaaagctgg atgtttatgc atccctgtcc ctggtgtctg agcgcaatca
     3481 ctatgtacgc ccgaagttaa atgaaaaagg tgtcattgat atcaaggatg gcagacatcc
     3541 tgtggtggaa cagatgatca ctaatgacat gttcattgcc aacgacacct atctggataa
     3601 tggaagtcat tgtatcagca tcatcaccgg tccgaatatg gcaggaaaat ccacatatat
     3661 gcgtcagacg gcactgatcg tgctgatggc acagatcggt tgtttcgtgc ctgccagaag
     3721 tgccaacatc ggcattgtag acagaatctt tacccgtgtg ggtgcatcgg atgacctggc
     3781 cagcgggcag tccacgttca tggtggagat gaatgaggtg gcgaatattt tacggaatgc
     3841 cacatccaaa agtctcctga tcctggatga gatcggaaga ggcacgtcca cctttgatgg
     3901 cctgagcatt gcctgggcag tgatcgaaca tatcagtaat cgaaagcttt tgggagcgaa
     3961 gacactgttt gccacccatt atcatgagct gaccgagctg gaaggcaaaa tgaacaatgt
     4021 caacaactac tgcatcgcag tcaaggaatg cggagacgat attgtattcc tgcgtaagat
     4081 cgtaaagggc ggtgcagaca agagctacgg tatccaggtg gccaaactgg ccggtgtgcc
     4141 ggatatggtc attgaccgtg caaaagagat cgtggaacag ctgtccgaca atgatatcac
     4201 cgaaaaggtg cagagtatcg ccattgataa taagggcgat gggaaagcaa aaaaacagcc
     4261 aaaatacgat gaagtggatc tggcgcagat gtctctgttt gatacggtga cggacgagga
     4321 tgtgctgaag gaactgatgg agatcgaagt aacgactctg acaccgctgg atgcactgaa
     4381 tactttgtac cgcctgcaga ataagttaaa gaaccgctgg aacgcctaga aagcataagg
     4441 gaggagattt atgggaatac atgtactgga ctcccggacg atcgacagga tcgctgccgg
     4501 tgaggtggtg gagagaccag ccagtgtggt aaaggaactg gtggagaatg ccattgatgc
     4561 cggatctaca gcaatcacgg tggaggcaaa ggaaggcggc attgaattca tccgcgtgac
     4621 ggataacggc tgcggtatcg agagagatca gcttcctacc gcgttcctgc gccatgccac
     4681 cagtaaaata gaggatgcgg atgatctgaa ccatattgcg agtctgggat tccgtggtga
     4741 ggctttatcc agtatcgcgg cagtttcccg ggtggagatc attaccaagc gaaaggaaaa
     4801 tctcacgggt acccgcatgg tactggaagg ggcgcgggag caatccattg atgaaatcgg
     4861 tgcaccggac ggcacaacgt tcctcatgcg gaacctgttt tttaatacgc cggtacgccg
     4921 gaagttctta aaacagcctg ccacagaggg cagttatatc acggatctga tggaacatct
     4981 ggcattgtcg agaccggata tttcgtttaa attcgtactg ggaaaccaga ccagattcca
     5041 tacctccgga aacggtgacc tgcgggaggt aatttaccgg atctacggca gggagattgc
     5101 cgcagagctg gtgcctgtcc agatcgaaga gaacggcatc aaagtcgaag gatatctggg
     5161 caaaccggta ctggtgcgtt ccaaccgtaa cttcgagatc tattttatca acggtcgttt
     5221 tatccgcagc ggtgtgatcg ccaaggctat cgaggaaggc tacaagcagt atctgatgca
     5281 gcataagttt ccactgtgcg tactgcatat caccatggat acggagacgg tggatgtgaa
     5341 cgtacatcct tccaaaatgg atgtgcggtt ctctgacggc atggcatttg ccagaatgct
     5401 cagtgagaag atcgccatca ctttgcggaa ccgggagatg atcccggatg cttctctgga
     5461 ggatgaaaag gcaatccaga agaaagaaca ggaagagaga agagcttcct ggaaagaaca
     5521 cacaccggaa gtatttgaaa aaaagagaga agaaagctat cgtgtcatgg aggcagcaaa
     5581 atatgaagcg gcttccaggg accggagaga atttatacag aaacctgtct ggcaggaaaa
     5641 tcatgccggg gtacagacca gtatccgcaa gccggagcta atagagaact ccacagtggg
     5701 tgttgcagga actgtgacgg tgaaaaaaac gcaggccgtc ctgtacggta atacaccaga
     5761 agtttccaaa gcagtcagca ccacagccgt accgcagaaa gatgatttct tcgtggaggc
     5821 agtgccgccg gaagacagga agaacccggt acaggaacag gtggccacaa caactgcttc
     5881 cggacagccc acggcatcgt cgcaggatgc agctgccgcc ccggaaccgg agatcaaaaa
     5941 tacccagcag ttgaatctgt tcgaggaaaa actgttgtcc atgcagaatc gcagccgtta
     6001 ccggattatc ggtcaggtct ttgataccta ctggctgatc cagtttgaag acaaattgtt
     6061 catcattgac cagcatgcgg ctcatgaaaa ggtaaaatat gaacgcctga tgaaacagtt
     6121 ttatgaaaag agtgtggtgt cccaacgtct gatgccggcg attattgtca gcctgacagg
     6181 gcaggaggaa agcattctgc acacctatga ggatgctttt gcacagctgg gatttgagat
     6241 tgatgctttc ggaggcaacg aatatgccct ccgtagtgta ccagtggatc tctacggatg
     6301 cagtgagagg gaactgttcc tggcagtgct ggatgaactg tcccaggaga ccggcaatcg
     6361 cggaagtttt caggtcattg aggaaaagat tgcatccatg tcctgcaagg cggcggtcaa
     6421 aggcaataac cgtctgagct ttcaggaggc ggagaaattg atcgacgaac ttctgaccct
     6481 ggataatccc tacaactgtc cccacggcag acccactatt attaccatga cggagacaga
     6541 tctggagaag aaatttaaga ggatagtata atgcgtttac tgatcaaaca gagagtgttt
     6601 tcctggacag attcctatga tgtctatgat gaaaatgaaa atccaaaata ttttgtaaaa
     6661 gcggagtttt tctccctggg acaccagctg catgtatacg acagaaataa ccatgaactg
     6721 ggagtgatca gacagcggct gcttaccttt cttccggcct ttgaaatcga gatcggtggc
     6781 cgggtccgtg gtgaaatccg caaacagttt tcttttttca agcccagata tgagatcgac
     6841 tataacggct ggcgcgtgga gggagatttt ctgggatggg attatgatgt atacaatgga
     6901 tgcagcagta tcatccatat ctccaaagaa ctgtttcact ggggagatac gtatactatt
     6961 aatttctccg atccacagga tgaattggag ggtctgatgc ttgtcatcgc catcgatgca
     7021 gcaaactgta cccagaacaa cggatagcag gataaaaaga gtaaggaaaa aataatgtcg
     7081 gtaatacaga ataaaaagaa accactggtg gtcctgaccg ggccgacggc ggcgggaaag
     7141 acaaaactgt ccatcgcgct ggcaaaagcc ataaacggag aaatcctgtc ggcagattcc
     7201 atgcaggtat ataaacatat ggatatcggt tctgccaaga tccgtccgga ggagatggag
     7261 ggggtgcccc atcatctgat cgatattctg gagccctggg aagaattcaa tgtagtagtg
     7321 ttccagaaac attgtctgga atgtatggag cagatatatg acaggagcca tatccctatt
     7381 ctggtgggag gcaccggatt ttacattcag gcggtgcttc ggggcattga ttttacggaa
     7441 aacgaagaga acaccgaata tcgaaaaaaa ctggaacttc tggcagaaga acagggaccg
     7501 gagagtctgc atgagatgct gaagaaggtc gatcccgtat cggcggagaa tattcatgcc
     7561 aataatatca agagaaccat ccgtgcttta gaatattatg aactgacagg agaaccgatc
     7621 tctgtacaca acgagaggga aaaagaacgg acaagtccct ataatttcag ctattttgtg
     7681 ctgacggacg accgggaaaa gctgtatgca cggatcgagg acagaattga tgaaatgatg
     7741 tcacagggtc tggtggatga agtaagacaa ctgaaggata tgggatgccg taagggcatg
     7801 acgtccatgc agggactggg ctacaaggag attctggaat atctggatgg agaatgcagc
     7861 ctggaagatg cggtctatac cttgaaacgg gataccaggc attttgccaa gagacagctg
     7921 acatggtttc gcagagagag tgaagtcacc tggatcgaca agggccggtt tgattataac
     7981 gaagagaaaa tattggaata catgatcaga gaattaggag aaaaagagat ttatgagaag
     8041 cagtaatgaa gagatctacc tgtctatggg aatcagcaag accgtatatg attacggctg
     8101 caccattgag aaaagtttaa gagaccgctt tgatgaagtg gatagtaacg cggaatacaa
     8161 tcagttaaag gtggtaaatg ccatgcagca gcatcaggtc agtgaggcct gcctgttggg
     8221 cactaccggt tacggctaca acgatcttgg aagagatacc ctggaggctg tctatgcagc
     8281 gactttccat acggaggatg cactggtgcg ccctcagatc acctgcggca cgcatgcact
     8341 ggcattggct ctgatgagca atttaagacc tggagacgaa ctgttgtccc cggtaggcaa
     8401 accctatgat acgctggaag aagtcatcgg tatccgcccg tcgaagggat cgctggcaga
     8461 atacggtgtg acttatcgcc aggtggatct gctgccggac ggaagctttg attacgataa
     8521 aatccgtgag aatatcaatg agaaaactca tctggtaacg attcagcgat ccaagggcta
     8581 ccagaccaga cccaccctgt ccgtacagcg gatcggcgaa ctgatcgcct tcattaaagg
     8641 cattaagccg gacgtgatct gcatggtgga taactgctac ggagaattcg tggagaccat
     8701 agaaccttcc gacgtggggg cggatatgat cgtaggatct ctcatcaaga accccggagg
     8761 cggactggct cctatcggcg gatatattgc tggaaagaaa gaatgtgtgg aaaatgcggc
     8821 ataccgtctg acctctccgg gactgggcaa ggaagtcggc gcttcccttg gcattttgaa
     8881 agatttttat cagggatttt tcctggctcc cacggtgacg gcgggagcac tgaagggtgc
     8941 gatctttgca gccaatgtct atgaaaaatt aggttttgcg gtagtaccga acggcagtga
     9001 gagccgacac gatatcattc aggcagtgac cttcggtcag cctgaagggg ttatcggctt
     9061 ctgccaggga attcaggcgg cggcaccggt agacagtcat gtgacaccgg aaccctggga
     9121 tatgccggga tatgatgcac aggtcattat ggcagcggga gcatttgttt ccggttcctc
     9181 cattgagctg tctgcggacg gccccatcaa gcctccgtat gcagtgtatt tccagggcgg
     9241 actgacctgg cagcatgcca aattcggtat tctgaaatcc ctgcaggtac tggtacaaaa
     9301 aggtcttgtg acggaagaac agatccggaa agcaatgtag aactacattg aaaaatacga
     9361 atcctgtaga aaaagcgcaa aataaaatct actaaatttg tgtacagaag aagctttttg
     9421 tgatagtata aaaagacgta tggaagacag gctcacgggg agactgttca gccatggaga
     9481 gtacacagaa gaaagaagag gaactacctt gaagagaatt aattgggttt tgatcgtcct
     9541 ggtactgatc ggatttgtga tcggcagatt tatagggact tatttcgacg gtacttttct
     9601 gaattacgga cagaatttcg gcctcagcag tccagtggaa ctgaatctcg gtttcattat
     9661 tctgactttc gggctggagt ttaatatcac gatcgccagt gtgatcggcg tgatcatagc
     9721 gttcgtggta taccgtttta tccgttaggc agcatacgtc ggagcaggca gaaggagact
     9781 tatgcccgag aagaatgcac aggtaaatac agaccgggga cttccctatg agagatttct
     9841 gcagttcgga ccggagaatc ttacggaggc ggaattgctt gcggtgattc tgcgaaccgg
     9901 gacgaaggag gattccgcgg tggcgctggc tgagaaggta ctggcactgg ccacctatcc
     9961 gagagaagga ctgctgggtc tgcatgatgt gagtgtggaa gaactgatgg agatccgggg
    10021 catcggcatg gtgaaagcgg tgaaactaaa atgcatcgcc gaactgtcca acaggatgag
    10081 ccgggccaaa gccagagagg ggatctgctt caccagagcg gatcatgtgg cggaatattt
    10141 tatggaaaaa ctcagacaca gggataccga atgtgtgtat ctgctctgtc tggatgccaa
    10201 aggacagctg atccgtgaga agaaattgtc ggacggcagc gtcaatatgg cactgatctc
    10261 tcccagagag atatttttgg aggcgttaaa ggacaaggca gtcagtctta tcctcgtgca
    10321 caatcatccc agcggagatc ccactcccag cagatccgac agggagctga cggaaaatgt
    10381 ggcaaaggtg ggaaaccaga tggacattcc gctgttagac cacattatta ttggcgataa
    10441 caggtatact agcttcagag agcaaaaatt tttgatataa gaaagggata acgacagtgg
    10501 caaacaatgc atttggtatc gaccttggta ccaacaatat aaagatctat aaccgcaatg
    10561 atgacaatat catgatcgaa aaaaatatga ttgccatcga gaataagaat actttatttg
    10621 cttatggtaa ctctgcattt gaaatgtatg agaaagctcc cagcaatatt catatttctt
    10681 atccgctttc caatggtgtg atcgcggata tcaaaaatat ggagacgctg gtaaagtatt
    10741 ttatcggtga tctgcagaag ggcaatacca agccttctga tttcttcatt gcagtgccta
    10801 cggatgttac cgaggtggaa aaaagagcat tctatgatct gatcaaagga gctaacgtaa
    10861 aggccaagaa gatcatggta gtggagaagg ccgtggcaga cggtctgggt ctggatatcg
    10921 atgtaaaaaa ttcccagggt gtcctggtgg tgaatgtcgg ctatgagacc acggaaatct
    10981 ctatcctgtc actgggcggt atcgtactga gccgtctgat caaggtgggc ggcatgaaat
    11041 ttgatgatgc catccgtgcg gcagtcagaa gagaattctc tctgatcatc ggtggcaaga
    11101 cggctgagaa tgtgaagatt gccctgaagg aactggaaga agaaggaaag ggtgctgtag
    11161 tatacggcag agacattgtc accggtctgc cggtggagcg tgagattccc acaagaatgg
    11221 tggacgagtc tctggaagag cactttaata ccattattga taatgtaaag gtgatactgg
    11281 agagaacacc ccctgaactg gcagcggata tttaccgtca cggtatttat ctgaccggag
    11341 gagcatccca ggtaagtcat ctggcagaga gactggcaac cggtaccgga ctgcatgtga
    11401 atgtggctga gaatccgctg accagcgtgg caacaggact tgccaagatc atcaaggatg
    11461 ataactataa aacggtagct tatgcaattg aagggatgag taaatgagcc cagtcgtaaa
    11521 aagaaaaggg gagaggttta ctttaccgag taaatatctc ctttttattt taactatcct
    11581 ctgcaccatc atgatgctgg tgacctttgg aaccaatgta tttaaccgac cgttcaacag
    11641 agtggtggga tatgtgatcg taccctttga acaggggatc gccaaggccg gagaatggct
    11701 ggcaaatcgt tccgatgaac tggtgcagat ccggacgctt ttagcggaga acagtgccct
    11761 gaaggagcag gtggcttcct tgacggaaga gaacactctg ctgcagcagg acaaatatga
    11821 actgaatgaa ctgagagggt tgctggagct tgatgaagaa tacggggact acaataagat
    11881 cggtgcccgg atcatcagca gagattctgg caactggtat tcttcttttg tcattgacaa
    11941 aggctctaat gatgggctgg cggatgatat gaatgtgatc gccggaggcg gtctggtggg
    12001 acgcattacg tccgtaggac cgaactggtc ccgggtgact tccattattt ccgacaattc
    12061 taatgtcagc ggtatgacac ttgccacggg tgacaatctg atcgtatccg gagatctgca
    12121 gatgatggcc cagggagtga ttcctttcag caagctggtg gacagccagg atgcagtggc
    12181 agagggagat aaggtagtta catccgatat cagtgataaa tatcttccca atatcctgat
    12241 cggctatatc agtaccatca ataaagatac caacaatctg acgaaatccg gatacctgac
    12301 tccggtggtg gatttcgagc atctgagtga ggtgctggtg atcacggaca agaaacagca
    12361 ggttccggga ggtggcgcag atggagaata acagcagctt cctgcgcaag attgtagtgg
    12421 ttcttctgat ccttttattt tttgtcttgc agtgcagtgt atttaacagc ctggcaatgg
    12481 gcggaatcat tcccaatctg atgatcattc tgacttcttc cttcggattt atgagagggg
    12541 agaaggaggg actgctgatc ggattttcct gcgggctttt atgtgatatt tttttcggaa
    12601 gtttcctggg attttatgcc atggtcatga tgtacatagg ctttgttaac gggaaattca
    12661 gccggatctt ttatccggag gatatcaagc tgccgttggc gcttattatt gtcagcgaca
    12721 tttcttatgg attgatttgt tacattctga tgtttctttt gagagggcgt tttcatttcc
    12781 cgtattattt tacaagtgtg atcctgccgg aggcacttta caccatcgtt gttacgatta
    12841 ttttgtatcc tctgatcctg aagattaatc agaaactgga ggagaaggaa aaaaggagcg
    12901 cacagaaatt tgtttcgtga aattagagat cacattatta atattgtgac cagcagactg
    12961 acagtactta tgatactgtt tgcttttttt gccggtgttc tgctttaccg ctgtttcaac
    13021 ctgcagatcg taaacggaga agagtatctg aacgacttta tccttcagat cgagaagacc
    13081 agggatatat ccagtaccag aggacggatc ctggacagga acggcaatgt actggcggaa
    13141 aatgaactgg cttattctgt gaagatcgag gatgtgtttg agtccagccg gaataagaac
    13201 gcaaaggtca atgacgttgt ctatcggctg ataaagctga tcgagaaaaa cggtgacagt
    13261 gtgatcacgg attttaagat cattattgat gaagacggcg attatgccta ttccgtggat
    13321 ggcacggcgc tgcttcgttt tcaggcggac gtgcttggat acagaaccat agacgagctt
    13381 agtgaagaag aaaaagccat gacagcccag gaactgatag agtacttaag ccgtgccaag
    13441 ggctttgcta tcggtcagta cgaagatccc gagaacacca agaccccctt cgtgccgggg
    13501 aaaggttata ccaaagagga gtggctgcag atggtaacca tccggtatgc catgaatctg
    13561 acctccttcc ggaaatatgt gggaaccact gtggctacca atgtcagtgc ggagacagtg
    13621 gcggtgatca tggagaattc cgaccagctg gacggtgtgt ccatcgtaga ggatacggtg
    13681 cgccactaca tagacagcaa atatttcgcc catgtgctgg gatataccgg caaaatctct
    13741 tccgatgaac tggcagagtt aaatgaccag gtggtgacgg aaggcggcct ggaggatacc
    13801 tataccatca atgatgtggt aggtaagagt ggtattgagg catatatgga gactaccctg
    13861 cagggtacca agggcagtga aaaagtggtg gtgaacaata ccggtaaggt gatcactatt
    13921 ttagagcgga aagaggcaca gccgggagcg gatgtctacc tgaccatcga taaggatctt
    13981 acggaggcgg tatataatat catcgaacag aagctggccg gtctggtggc aagcaagatc
    14041 atcaacgcca aagaattcaa tctgccggag aatgccaaga gctcgtccat taagatcccg
    14101 atctatgacg tgtattttgc catgatcaac aacaatatcc tggacagaaa acatttcgag
    14161 gcagaagatg ccggagagac ggaaaaggca gtctatgccg cttatctgga gtataagcag
    14221 ggtgtttacg acagactgac ttacgaactt acagaagggg caactcccta tagcaagctt
    14281 tccaaggaat atcaggttta tcagagtaat atcgtaagcc tccttcggga ggaaggcgtg
    14341 atcatgaagg aactggtgga tgataacgat gctacgcaga cggcctgggc gaaggaggaa
    14401 gtgatctcct taaaggagta tctgcagtat tgcatcgcca tgaactggat cgatgtcagc
    14461 aagctggatc tgaatgacaa atattcggat tctacagaag tttatgacaa actgttggag
    14521 tacaccatta atgccatcga ccataccacg gagttccaga aacgtttcta caagtatatg
    14581 cttttaaatg acaagatcac cgggaaacag atctgtatgc tcctgtgtga acagcaggtg
    14641 gtggacatcc cggctgagga tgaagaaaca ctgtattccg gtaaattgtc ggcatatcag
    14701 tttatgatga accggatcaa taatctggag atcacaccgg cccaactggc actggatccc
    14761 tgcaatgcct ctgtagtcat tacggatgtc aatacgggag atgtgctggc tatggtgtct
    14821 tatccgggat atgacaataa caaaatggcg aataccgtgg atgcggagta ttatgcgcag
    14881 ctgaatgcgg acaagtccag cccacagctg aattttgcca cccagtataa ggcagcaccc
    14941 ggttccacgt tcaagattgt atccgctaca gcgggacttc tggagaatgt gattaatctt
    15001 cagagccgtg tgaactgtgt cggtactttt accgagatca caccatcccc cagatgctgg
    15061 aaaatttccg gacatggcta tgaaaatgtg acatctgcca tcaaggattc ctgtaactac
    15121 ttcttctaca atgtgggcta ccagctggct accagaaccg gaagctataa tgaggaagcg
    15181 ggtctggata ccctgagcaa atatgcggat ctgtatggac tgacagataa atcgggagtg
    15241 gagatcggag aatatgcacc ggatgtatcc accaaggatc cggtacgttc tgccatcgga
    15301 cagggcagca acagctacac taccgttgga ctggccagat atgtcactac cattgccaac
    15361 agtgggacct gctataatct gaccctgttg gataagacaa cagattccca gggcaatctg
    15421 ttgaaagagt accatgcaga agtgagaaat cagattgacc tgccgcagga atactgggat
    15481 gcgttccatc tgggaatgcg tcaggtggtg gaaaacaaaa agtactttag tgatctggcg
    15541 gtaaatgccg caggtaagac cggtacggca gagcagacag cttcccgacc aaaccatgcg
    15601 ctttttatct gctatgcgcc gtatgagaat ccggagatcg ccattgccac aagaatacct
    15661 ttcggatatt cttcagacta tgcagcccag ttcaccagag atattatcaa gtattattac
    15721 ggactggcgg aagaggatga tctgatcaca ggtactgcgg atactctcga caatgcagta
    15781 tccaatgaga tgtaactatc cggagtcaga taacacagac agatgaatgc caaatgtggg
    15841 acttcggata ttaagaaaat aaaaagacag gtataagaaa atgaaagatg cagtattgat
    15901 caaaagctat cagagcggga tcactcttct gatgagggag gatgcttccc tggaagaaat
    15961 cctgcaggaa ctggcggtga aattcacaga ggcgaaaaac ttcttcggta cgtccaccat
    16021 ggcattatcc atggagggac gggaggtgac cgaagtggaa gaaatccgca ttctggatac
    16081 catccggaag aacagcaatg tgaggatcgc ctgtatcgtg ggccatgatg agaccacgaa
    16141 caagaacttt atcaaagctc tgcagcatat ggagaaaaag ctttccaccg gggacgaggg
    16201 gcagttctac aagggaaccc tcaaaaaccg ggaagtcata gaaaccgaga acagcattgt
    16261 ggtcctgggg gatgtctacc cgggcagtgc ggtgatctcc gcaggcaaca tcatcattct
    16321 gggaggactg tacggggaag cttatgcggg cggatctggc agagaagatt gttatattgt
    16381 agcgcttgaa atggagccgg aaagattaaa aatcggcgat ttcaaatata aaaccaatgc
    16441 gaaacagtcg aaatggggca ttcgtcccaa agtacaaccg aaaattgcac acctgaagaa
    16501 cgggaagatc gtatttgatc cccttacaaa agaattactg ggtacttttt aaaagaatcc
    16561 aggggacgac aaaatcaaac aggaggaatc caaaatggaa caaaacgttt ccacggagaa
    16621 gaaaagctcc gaagtcattg tcgtcacttc aggaaaaggc ggtgtaggta agaccactac
    16681 atccgcaaac gttggaacag gtcttgcaaa agcgggcagc aaagtctgcc tgatcgatac
    16741 ggatatcggt ctgcgtaacc ttgacgtggt catgggactg gagaaccgta tcgtttacaa
    16801 tctggtagat gtggtcgaag ggaactgcag aatcaaacag gcactgatcc gggacaagag
    16861 acatccggga ctgtatctga tgccgtcggc acagaccaga gataaatcgg cagtcacccc
    16921 gggacagatg gtgaaggtga tcgaccactt gagagaacaa tttgattaca tcattctgga
    16981 ctgcccggcg ggaattgaac agggcttcca gaatgctatt gccggggccg acagggcact
    17041 ggtggtaaca acaccggagg tctcggccat cagagatgca gatcgtatta tcggactgct
    17101 ggaggccaac ggcttcaaac agatggatct gatcatcaac agactgcgca tggatatggt
    17161 aaagcgcgga gatatgatgt ctgcggatga tgtggtggat attctggcgg taccgctcat
    17221 cggaatcgtt ccggatgatg agaatgtagt catcgccacc aatcagggag aaccattagt
    17281 cggaagcgat acaccggcgg gtaaggctta ccgcaatgtg gtggatcgtg tgctgggaaa
    17341 agaaattccg ttcctggatt tcgaaaaagg agtatctttt tggacaaaag taaccggtat
    17401 ttttcggaaa gcataggaag gggacgggat gaatatgaaa catttttttc gcaggaaaag
    17461 ctcctgccag gtggcaaagg accgtctgaa aatcctgctg atatcggacc gggtgaactg
    17521 ttctccggag atgatggaac tgatcaaggc ggatattaca aaagtgattt cgaaatatat
    17581 gcggatcgat acggatcata tggaaatcga gatacaggca aagggcagta agggcggacg
    17641 gaataagagc cccagcctga ttgccaatat tcccattatt gatttacaga agactgcgaa
    17701 aatgccataa ggagcataaa gaacagaaag gtcaaacgga tgtttaagaa ttatagatta
    17761 cgggattatg actttaaact gatcatactg atacttgcac tgtccatcat cggcatcata
    17821 accatcggca gtgcggaacc tgcccagcag accaaacaga tggcgggtgt agttgcgggg
    17881 cttgttatca tgatcgccct ttcttttttt aattattccg tgatcctgaa gctttactgg
    17941 ctgatgtaca taggcaatct ggtactgctc ggactggtaa tcctgatggg tgaagaaggc
    18001 aacggagccc agaggtggct taagatcggt ggactgcaat ttcaaccttc agaacttgca
    18061 aaaattttat tgattttatt ctttgcacag tttattatga aatataagga gaagctgaat
    18121 actttccctg tgatcgcatc gattgcggta cttatgggtg ttccctggat catggtaaag
    18181 gaacagccgg cgctgtctac cagccttgtg ctgatcttta ttttctgcgt gatcctgtat
    18241 gccggaggca tcagttataa gctgatcttc ggagcattgg cggtggcaat tcctgcagtg
    18301 atcatactgg tatctctggc aatgcagccg gacagtacca tattggagac ttaccagaaa
    18361 aatcgtatcc ttgccttcgt caatccggag gaatattcca cggatctggc ttaccagcag
    18421 ttgaactctg taatggccat aggctctgga gaactggacg gcaagggcta taaaaacaat
    18481 gagatcacat cggtaaagaa tggcaatttc ctgtctgaag cggaaaccga tttcatattc
    18541 gctgttatcg gtgaagagtt tggttttaag ggaagtatcg tagtgatcat actgctgatg
    18601 ctgatggcaa tggagtgcgt ttctatcgcg ggaaaggcga aagataccgc ggggacgatc
    18661 atagcggcgt caatgggggg actgatcgct ttccagagct ttgtgaacat cggagtggct
    18721 acgtttattt taccgaatac aggacttccg ctgccttttg taagctatgg cctgacctcg
    18781 ctgatgagtc tgtatattgg aatgggtgtt gtactgaacg tacgattaca ggcgaagaga
    18841 agctagacaa aagaaatcat acattacaca gagaaggggt atggactata tgaacattgc
    18901 actgatcgca catgatgcca aaaagaaact gatgcagaat ttctgcattg catatcgcgg
    18961 aattttaagt aaaaatgaat tatatgccac aggaactacc ggcaggctga tcgaagaggt
    19021 caccaacctg aatatacaca aatatcttgc tggacatctg ggaggagaac agcagatcgg
    19081 tgcacagatc gaacacaacg agatcgatct ggtgatcttt ttgcgggatc cactggcacc
    19141 caaggcccat gagccggatg taagcaatgt aatgcgcttg tgtgatatgc ataatattcc
    19201 gttggcaacg aatctcgcca gcgcggaact gttgatcaag tccttagaca gaggagattt
    19261 agagtggcgt gaaatgtaca aataaagttc gtcggatctg cttctgcatg tccttgatcc
    19321 tctgcatgag tctgcttacc ggatgcggta atgtggcgta tacatttccg tacgatgtca
    19381 attccagtgt cagcagtttc aatgtgctgg caggaaccgg agcgggcagg gcagagccct
    19441 ttgctgcgga tctgtgtgtg gtgacggaga accagacggg ggatggcagc gtagacatgt
    19501 cacaggcttc ggccgctgta ctgttcgacg taaacaataa aaaggtatta tattccaaaa
    19561 atgcacatga aagattgtat ccggccagcc tgaccaaggt aatgacggca ctggtggcat
    19621 tacagaatgc gtcaccggat actgtgctga cagctacgga tagcgtaaag atcacggaat
    19681 ccggtgcaca gctgtgcgga ttaaaagccg gagataccat gacattggat caggcattac
    19741 atattctgct gatgtattcc gccaacgacg cagcaatgct gatcgcggag aatatcggcg
    19801 gcagtgtaga tcattttgtt gagatgatga atgaggaagc acagagactg ggggctacaa
    19861 ataccagttt tgccaatcct catggtctgt cggatgacaa tcattatact tcagcctatg
    19921 acctgtatct gatctttaat gaagcaatca aatatgattc ctttaatgag atcattcaca
    19981 tgacaagcta ccaaaccgca tattatgaca aggacggaaa agccaaggaa cttacggtga
    20041 acagcacaaa ccgttttttg cggggagatt ttcaggcacc ggagaacatt acagtcatcg
    20101 gcggcaagac aggtacgacc aacgcggcgg gacattgtct gatcctttta tccagagaca
    20161 caagcggagc tccttatata tcggtaatcc tgaattcctc agccagtgat gtgctgtacc
    20221 cggaaatggt acaactcctg gagcaaatca ataaataact gttgtttttt gaggggttat
    20281 aaactataat aaagtaaata ggttgcgtga agttcctata aattttacga caggagggtt
    20341 acaccaatgg ttcagttgat tgtaggcaaa aagggcaagg gcaagacaaa acagctgttg
    20401 gacaaagtca acagcgaggt aaaggatatt gcgggaagta tcgtatatct ggacaaaagt
    20461 acgaaacata tgtatgaact gaacaacaag gtgcgtctga tcgacgtttc ccgttatatg
    20521 atcgagaatg aaagcgaatt tctgggattc gtatgcggta tcatttctca ggatcatgat
    20581 ctggagcaga tgtattttga cagctttctg aaaatcgcag cactggaaga caaagacatt
    20641 tccgcagttg ttgaaaaact ggagagaatg agtgatttct tccaggtgga ttttatcctc
    20701 agtgtatcca gggacgaatc cgaattaccg gagtctgtga aggataagat cattgtatct
    20761 ttgtaagaag aacgaattat aattttcacg tatgagaagc ctcggatgcc aaccggggct
    20821 tctcaaacgt aagggagagg aaaggaggtt ttccaatggc agaacaaccg agaaaaataa
    20881 aaaaatacag aaaacctttg aacctcaata tcggtatgct catcttcgga gctattttta
    20941 tttatgtaat catttgtgtg atactgtatt ttcagacaga ccacatcgtg cgttatgaag
    21001 taacggaagg atcactggct accaataata tttaccgtgg agtgtcaatc cgcacagagg
    21061 aagttgtaag tacggatact gccggatatg tgaattttta tgcaagagaa ggcgaacggg
    21121 tggccaaggg agacactgtt tacatcgtgg acgagacggg ccggctgacg gaagaactgg
    21181 aggatgccag ccttggagaa aataccctga gtgaccagga actttcggaa ttccgtaatg
    21241 aaatcgtgaa ttttatgcac gggtatgatg acaaaaatta cgagagtacc tatgatttta
    21301 aatattcttt gaagaacacg gtgctgaaac tggccaatgt aaatatgctg caaagcattg
    21361 aaaacggaaa taacggttcg gctgcaaata ttgtaaattt ctgttatgcg cccaccaccg
    21421 gtatcgtggc ttactggacg gacggttatg aaaatctgac cgtggaaggt gttacccagg
    21481 aagtatttga taacaaaaat tacgagaaga aacagatgct cggtaatgaa ctgatggctg
    21541 tgggagatcc ggtgtacaag ctttccacgg aggaaaactg gtcggtggtc attcccatcg
    21601 atgcagagcg aggcgcagag atccaacagg aggattatgt caaagtacgt tttctgaaga
    21661 atcagtatga gtcctggggg caggcaaagc tgtttaccgg cagtgatggc aatacatttc
    21721 tgtcacttac ctttacaaat tccatggtta cgtttgtatc cgacagattt ctggatatcg
    21781 agctgatatt gaatgatgag accggactga agattccaaa ttcttccatt gtggagaagg
    21841 aatttttcct gatcgatgaa gattttgtca cgaccaatga aaatacggga acccagggtg
    21901 tgatcagaca gtgctaccgg gataacgggg acatttccag tgaatttgtg gaggccggtg
    21961 tgtacagcta cgatgaaaat gaaggcgtgt actacatgga tacttcggtg ctgaaggccg
    22021 gagatgtcct gtacaagaca gacagccagg agacctatac ggtaagcaag cgtgcaacac
    22081 tgattggtgt gtataatgtg aataagggat atgctgactt caagcagatc aatattctgt
    22141 atcagaatga cgagtattcc atcgtaaaag ccaatacgac ctatggcctg aatgtatatg
    22201 actatattgt actggacggt tcttcggtat cggatgatca gatcatcacc aatatcaaga
    22261 aaacagatac cggcaggacg gatattccta cagacagtgt agaaccggga gtaagcgaca
    22321 atataccgga gagcacagag ccacttccag cggagaccag agaggaaccg acgcaggaga
    22381 ccacacagga agcaacacag gaaagtcaga caccggaaag ctagaaagga tggcggatac
    22441 tatggaagag accatacaga gcatggaact ggcacaaaat tacaaaacgg taaaagagaa
    22501 cgtcacaaaa gcctgtgagc aggcgggcag atcggagcag gaagtgacgc tgcttgcagt
    22561 cagcaagacc aaaccggtgg atatgctgat ggatgtctac cgtgcgggag ccagagattt
    22621 cggggagaac aaggttcagg aactggtgga caagatccct cagatgcctt cggatgtcag
    22681 atggcatatg atcggccatc tgcagcgcaa taaggtaaaa tatattgtag ataaggttta
    22741 cctgatccac agcgtggaca gcctccggct ggcggaagag atcagcagag aagccgtgaa
    22801 gaagcaggtg gaagtcaata tcctgatcga ggtaaatgtg gcacaggagg aaagcaagtt
    22861 cggaactacg gtagaagaaa ctgcaacact gatccgtgag attgcaacgc ttccgggaat
    22921 acatatcaga ggattgatga cgatcgcccc ttttgtggag gatccggaag aaaacagggg
    22981 ctattttcag aaacttcggc aattagctgt tgacatcgga aataaaaaca ttgataatat
    23041 ttctatgaat attctttcca tgggaatgac cggcgattat atggtggcca cgcaggaagg
    23101 agccacgatc gtcagagtcg gaactggtat tttcggagaa agagattata gtaagacgat
    23161 ctagaaagag attgaataat aaaggagaca cacaacaatg ggtgtaatgg ataagttcct
    23221 gaattatatg aaactgaacg atgaagagga tgaaggtttc tatgatgacg attatctgga
    23281 tgatgaggaa gaagtagaac cggtaaagaa attcagcccg gccaagagca gacagcaggc
    23341 acaggaggat gacgaaccgg aagagaagag taagcgttcc tcacagccta aggtaacacc
    23401 gatccaccgc accgtgacca agaaaatgcc tggaaccggc atggaggtct gtgtgatcaa
    23461 gcctacttcc gtagaggatg caagagagat cacagagaca ctgttggcca accggacagt
    23521 ggtacttaat ctggaaggcc tggacgtgga tatcgcccag cgtatcattg actttacttc
    23581 cggatcctgt ttcgcaatat cgggtaattt acagaaaata tcccattata ttttcattat
    23641 tacgccggcc agcgtagata tctccggtga ttttcaggat attttcggcg gcgccggatc
    23701 ttttgaaatg ccgatcaaca atggaatgtc ataaaatgtc aaatgccatt aaaatgtcat
    23761 atgaagataa cttcctgtgg gcaactgcag gaagtttttt aaaaacagat ataaattata
    23821 caagaaagaa cagtttataa ggaactgaag ggtgaacaac atggcacaga gattatcggt
    23881 gctttataac aaaaagcctt gctacgatat tgtattccag cagtcttttg acggcctgtg
    23941 ggaagaactg gcagagcttg gcgcagcaga caagagatta tgtattgtga cggattccac
    24001 tgtagacgga ctgtatgcgg cacagattct taagtttctg gagggaaaat gccgtaaggc
    24061 tgtgaaatat gtgttccccg caggagaaga acacaagaat ctggataccg tgagggacgt
    24121 gtaccgtttc ctgattgaag agggttttga cagaaaagat atgctgctgg cactgggcgg
    24181 cggcgtctgc ggtgatctga ccggatttgt ggcggctact tatttaagag gcattgattt
    24241 tgtacagatt ccaactacat tgctggcaca ggcagacagc tctattggcg gtaagacagg
    24301 tgtggatttt gacagctata agaacatggt aggcgcattt aagatgccca gactggtata
    24361 tatgaacctg tccgtattaa aaacgctgga agagagacag ttttattccg gatttgcgga
    24421 agtaatgaaa tccgcactga tcaaggatgc gcccttctat gagtggctga tcgagaacat
    24481 gtatgagatc tgcgagcggg atctgaatac cctggaagaa atggtgatcc gtacctgctc
    24541 catcaagaaa atggtggtgg aaaaggatcc taccgagcag ggagaccgtg ctctcttaaa
    24601 tcttggacac acgatcggac atgctatcga gaaatacaag aattttgaat tgtatcatgg
    24661 agagtgtgtg gcactgggta cggtagctgc agcatatatt tcctggaaaa aagaaatgct
    24721 gagcatggaa gaattttatg agatccgtga tatgtttgta ccgttttatc tgccaatctc
    24781 cgtggacgat atcgatccgc aggagatctt gaaacttaca aaatccgata agaagatgga
    24841 agccggtacg atcaaattta ttctgttaaa gaagatcgga aaggcagtta tcgacagaac
    24901 cgttacggat gaggaaattc tggctgccat cgaagaaatc aattttagtg aagaggatgc
    24961 tcatgaataa ggaaaagaaa agtagtcgag ttgttatgtt gatcatagat gccctgattg
    25021 cagccgtact tctggtactg gatcagttta ccaagcatct ggcggtggta catctgaaga
    25081 accagccggc ctatgtgatc attgacggag ttctggaatt gcaatatctg gagaacagag
    25141 gatccgcatt cggtatgctg cagaaccaga agatatttat tctgtttgtg ggaatcgtat
    25201 ttcttgcggt gctgttgttt ttccttttca agcttccgga acagaagaaa ttccgaatcg
    25261 tgcatgtatt gctggcagtg atcattgccg gaggtatcgg caacatgatc gacagattcc
    25321 gactggatta tgtagtggat tttatctcct tcgtactgat caattatccg atctttaatg
    25381 tggcggatat ttatgtggta gtggctacca ttggcctgtt tattctgttt ttgttcgtat
    25441 acaaggaaaa ggatctggaa ttcctcaact ttaagcagaa tcgttatcgg gagatgaaat
    25501 aatggaagaa ctccgatttc agatagcaga tgagatggaa gacgaacgga ttgacaaatg
    25561 tatgagtctg cttgtggaga atctgtcccg ttcttttatc caaaagctga tcaaagacgg
    25621 aaatgtgctg gtaaacggta cgccggtaaa gggaagttgt cgtgtcaaat gtgatgatga
    25681 agtagtcttc tccgtgccgg aatctgtgga accggatatt gaaccggaga acatccctct
    25741 ggatatcctg tatgaggatg cggatctgat cgtggtgaat aagccgaagg ggatggtggt
    25801 gcatccggcg gcgggacatt acagcggcac tctggttaat gcactgatgt atcactgtgg
    25861 cagggatttg tccgggatca acggagtact gcgccccggt attgtacatc gtattgacaa
    25921 ggataccact ggatctgtca ttgcctgcaa aaatgacaaa gcacaccggt gcattgcaga
    25981 acagttgaag gaacattcca tcaacagaag ataccgtgcc atctgtttcg gagagctgaa
    26041 tcaggaggaa ggcaccatcg accagcccat cggcaggcat ccgaatgacc gtaaaaaaat
    26101 ggcggtgaac caccagaacg gaaaacgtgc cgtgacccat taccgggtat tacagagatt
    26161 tcagggatat acttatatag agtgtgtgtt ggagaccgga aggacgcacc agatccgtgt
    26221 gcatatggca agcatcgggc atccgcttct gggggatgaa gtctactcca accggaaaag
    26281 tcctttcaaa ttacaggggc agacacttca tgccaagaca ctgggattta ttcatcccac
    26341 taccggagaa tatctggaga tcgatgctcc gctgccggaa tattttgaac atcttctgac
    26401 agtgttggag aaataaaact tatagcttat gtttcgtaca gaaatgaggc gactatgaat
    26461 ctgtttgata ttttaggacc tgtaatggtc ggccccagca gttctcatac tgcgggagcg
    26521 gtccggatcg gttatatttc aggaaaatta cttcaggacc atgtggtgaa ggcggaaatc
    26581 cttctgcatg gttcctttgc tactacgggc atcggacatg gcacggataa ggcgctgatc
    26641 gcaggccttt tgggaatgcg gccggatgat atccggattc cggacagctt tttcctggcg
    26701 aaaaaggacg gaatggaatt ctctttttcc accattaccc tgaaagatgc ccatcccaat
    26761 acggcggttc tgcggctgac cggagaacat gggcggaaaa tagaagtgca ggcggcttcc
    26821 attggcggcg gccggatcct gatcgcaaaa ctggatggaa tcgaagtaaa tttctctgcg
    26881 gaaaagccga ctttgattgt acacaatgtg gatcagccgg gacatgtggc acaggtgacc
    26941 tccatgctgg ctgaaaagca ggtgaatatt gcaactctgc aactctacag ggacaagcgg
    27001 ggcggctacg ccgttatggt catagagaca gatcaggaag tgccggagga atcggtggcc
    27061 tggctggaac agctggacgg catcattaag gtgacctata tcaatgtgga ggagtaacta
    27121 tgtcctatca gtcatttgaa cagatattac agaaatgtca tacggaagga aaagaattct
    27181 ggcaggtgat tctggaagat gatatggcgg agcggaatgt gtcaaagcag gacagtatgg
    27241 agcatatgcg gactacctgg gatgccatgc tgctggcagc ggctacttat gaaggagata
    27301 ttaagtcctc cagcggactt gtcggcggtg atggccaacg gatggaggaa tacacacaca
    27361 ataccggtaa ggagtcacta tgcggggaat atgtagacga atgtattgca ggagcgttac
    27421 agatgggaga atccaatgcc tgtatgaaac ggatcgtggc agcacctacc gctggagcct
    27481 gtggggtatt gcctgcggtg ctggttccct attttcaaaa acggtgcagg gataccgaca
    27541 aaattctgaa agccatgtat gtagcggcgg ggattggaca gattatagcc acccgttctt
    27601 ccatcagcgg ggctgcggga ggatgccagg cggagatcgg gtccgccagt tccatggcag
    27661 ccggagctct gacatatctg cagggaggca actgtgaaca gattgctaca tcggctgcca
    27721 tggccatgaa gaatctgctg ggactggtat gcgacccggt agcgggactg gtggaggttc
    27781 catgcgtgaa acgaaatgtg atcggtactg tgaatgccat ggcctgtgcg gatatggcaa
    27841 tggcggggat cgtaagccgg attccaccgg atcaggttat ggatgccatg aaagaagtcg
    27901 gggataaaat ggatgcttcc ctgagggaaa cggcgttagg aggcctggca cagacggaag
    27961 aaggcatgaa aatagcagaa aaaatgcatg catcatatga aaattagtaa taaacgacta
    28021 aaaaaactga taattggttg attttttgtt aaatatcacg tataatgaaa atacaaaaaa
    28081 tgctagttat caagtacagc agaaagtggg tgtgtgtatg aatacgatta ttaccattgg
    28141 acgtcagttt ggttctgcag gccgtgaaat cggggaaaag gttgcagagt atttcggaat
    28201 caaatgctat gataaggaac ttcttatcag agcggcacag gaaagtggtt tctgtgagga
    28261 aatgatccag aaccatgatg agagacctac caattctttc ctgtataatc tggtgatgga
    28321 tacttattct ttcggctaca atgcatcctc ttttgtagat atgcctatca gccacaaagt
    28381 atttcttgca cagtttgata cgattaagaa gatcgccgat gaaggccctt gcgttattgt
    28441 aggccgttgt gcagattatg ctctggcaga ttacaagaac tgcatcaatc tgttcatctt
    28501 cggcgatgac gatgtgaaga ccaaacgtat catggccaga tatgatctga cggaagccaa
    28561 ggcaaaggaa atgatcacga agaaggataa gcagcgtcag agttactaca actattattc
    28621 ttccaagaag tggggcagag ctgatagtta cgatctgtgc atcaacagca gcatcctcgg
    28681 catcgaagga accgtaaagc tgatcattca gtatgtggaa gacttcgaga agaaaaataa
    28741 tgcagatatt ctggaaaagt agatatctga aatatttttc aaaaaaataa aattatatct
    28801 tgacgattag ggcacctcat gttattatac tacggtatgc cgcataacta ggtataagtg
    28861 tgtcggattt tagaatcctt taccaccaaa gttagcgagt aaataatagg aggtgcctta
    28921 ttatgtacgc aattattgca acaggtggaa agcagtacaa agtttctgaa ggtgatgtga
    28981 tcaaagttga gaagatcgat gcagaagctg gtaatactgt tacttttgac caggtgatcg
    29041 cagtaagtga caataccctc aaggttggtg ctgacgttgc taacgcaact gttaccgcaa
    29101 ccgttatgga gcagggtaga ggtaagaagg ttattgtata caagtacaag agaaaaaccg
    29161 gttaccacaa gaagaatggt catagacaag catacactca ggttaagatc gaaaagatta
    29221 acgcgtaatc gttatgacaa ctattatcat tcgtaagaac catgaaggga tctatcgtgg
    29281 attctactgc atggggcatg cggaatatgc gaagaagaga ttgttcggta aggaaccgga
    29341 tatcctctgt gcagcgattt ccgtgaccgt acagagtacg atcatggcgt tggacgagat
    29401 gatcggcgaa aaaatgcagc tgacgcagaa tgaggagacg ggatttatca aatgtgattt
    29461 cgagaattcc attccgcagg acaagaccgt atttctgatg gactttatgg tgttttcact
    29521 gcagaagctc agcagacagt acggggaaca gtatttgcaa gtaaaaatcg aggaggtgta
    29581 gaccatgttt aactttaacc ttcaattttt tgcacataaa aagggagttg gttctaccaa
    29641 gaacggtaga gattccgaat ccaagagatt aggtgcgaag agagctgatg gccagtttgt
    29701 aaaagctgga aatattcttt acagacagcg cggtactaag atccatcccg gtgtcaatgt
    29761 tggtatcggt ggagatgata ctctgtttgc tttagttgat ggcgttctcc gttttgagag
    29821 aaagggcaga gacaagaagc aggcttctgt atatcctgta gagaaataag ttgatttaac
    29881 cagggcttca aacatactga ttgtttgaag cccttttagt ccgtaaagag ttttatgtcg
    29941 gaaaggagaa caaaatgttt gctgacagag ctaaaattta cgtaagaagc ggtaaaggcg
    30001 gagatggtca tgtgtccttt cgccgcgaaa aatatgttcc cagtggcggc cctgacggtg
    30061 gcgatggtgg tcacggcggt gacgtgatct ttgttgtaga tgagggactg aataccctga
    30121 tcgatttccg gcacatccgt aagtacaagg ccggagacgg ggaagagggc ggcaagaaaa
    30181 actgccgcgg caaggacgga gaagatatca ttatcaaagt ccctgcggga accgtgatca
    30241 aagaagcaca gagcgggcag gtaattacgg atatgtcggg ggacaacaaa cgggttgttc
    30301 tcttaaaagg cggcaagggc ggtaacggca accagcatta tgctaccagt accatgcagg
    30361 cacccaagta tgcacagccg ggacaggctg cacaggaact ggaacttctg ctggaactga
    30421 aggtgatcgc tgatgtcgga ctggtaggat ttcccaatgt tggtaaatcc acgttcctgt
    30481 ccagagtgac caatgccaga cccaagattg ccaactatca tttcacgacc ctgaatccga
    30541 atctgggtgt agtggatctg gaaggaaccg gtggttttgt cattgcggat attccgggac
    30601 tgatcgaggg agcttccgag ggaatcggtc tgggacacga attcctgcgc cacatcgaac
    30661 gtaccaaggt gatcatccat atcgtggatg cagccggtac ggagggaaga gatcccattc
    30721 aggatatcta tgccatcaat aaagaactgg aagcctataa tccggagatt gcagccagac
    30781 ctcaggtgat cgcggcgaat aagattgacg ctatctatac ggaggatgga tccgatccgg
    30841 tagctgcaat ccgggcagag tttgaaccga agggcatcaa ggtgttccct atgtctgcag
    30901 taaccggtca gggcgtaaag gaactgttat acgaagtgaa cgatatgctt gctaacatcg
    30961 gagaagagac cactgtcttt gcgcaggagt acttcccgga gagtatgaca ggatctgtgg
    31021 atgatgctta caccgtaacc tatgatgaag aagaggacga gtacgtagta gaaggaccca
    31081 agatcgagaa gatgctggga tacaccaatc tggacagtga gaagggattt accttcttcc
    31141 agaatttcct gaaggatacc ggtattttag aggaactgga agcactgggt atccaggagg
    31201 gcgatacggt acgcatgtac ggtctgtcct ttgattacta taagtaagtt tgtgaaagga
    31261 aaagatagat tatgacaagc aaacagagag cttatttaat gagtctggcc agcaatctga
    31321 atcccatttt tcaggtgggt aaatccagcc tgactccgga actcaccgca gctattggtg
    31381 aatcctttaa taataatgaa ctgatcaaga tcggtgttct gaagaactgt tttgacgacc
    31441 ccagagctat tgcggagatg gtggcggagc gtacccattc ccaggtcgta caggtgatcg
    31501 gcaagaagat tgtattgtac aagcccgaca aggacaagcc gaagatcgaa ctgcccagat
    31561 aaggtcagag catagccgac gagaaccaat caagaatgat aactgcgtgc aaagggatga
    31621 ggaagcatga gcagaatagg cattatgggt ggcaccttta acccaatcca cataggacat
    31681 ctgatgctgg ctgaacaggc gctggagtcg gcacacttag accagatctg gatgataccg
    31741 acgggctgtt cttatctgaa gacaaaagag ggcattacgg ttctgcccgg gacagaacgt
    31801 tatgacatgg tacagcgtgc tatcgcagat aacgacagat tccgctgcct ggacattgag
    31861 ataaaacgtc ccggaaattc ctatagctat gagaccatgg agcagctgca cagagaatat
    31921 ccggagcacc ttttttattt catctgtggg gctgactgcc tgtttaccat ggaacaatgg
    31981 aaatgcgcag gccgcctgtt tgctgcctgc gagattatag ctgcggtacg gggagatgcg
    32041 gatatgaagg gaatgcagga gaagatcagt cagttggagg aacatttttc tgcgaagata
    32101 cagttgctct cttttcgaag aattgagatc tcttcttcgg aaatccgagc gagaagatcc
    32161 gaaggtcttt ctgtgcgcta tcttgtgccg gaaacagtga gggaatatat ggaagaaaag
    32221 gggctttacc tggatgagtg atatcttaaa aaaactgacc aaagagatgc aaaaagtaca
    32281 ggatcccaag cgctttaccc atacattggg ggtagaatac acagcggcgg cccttgccat
    32341 gcgatatagt gaagatgtgg agcgtgcaca gatcgcggga ctgcttcatg attgtgccaa
    32401 atgcatggaa aatgataaac tgttgtctgt ctgcgaaaag tatcatcttc cggcgacgga
    32461 tgtggaaacg cgcaatccct atcttttaca tgccagagtg ggaagctatc tggcgaaaaa
    32521 ggaatataaa atcgaagatc ctgagatctt aagtgcaatt ttatatcata ccacagggcg
    32581 tccgggtatg acacttctgg agaagatcat ttttaccgca gattatattg aaccggggag
    32641 aaaacaggca ccgaacctta cccagatacg ccacctggca tttacggatc tggatgctgc
    32701 cgtgacgaag attttagagg atacgctgga atatctgaaa tcgggggacg gtgagatgga
    32761 tcccatgaca gaagaaacct ataactatta taagaagaat ggataaataa aatagaaagt
    32821 gtgaggagtt agcatgagcg aagaatccag aaaaatggca aaactggccg tagaggcgct
    32881 ggaagataaa aaagcagagg atatcaaagt aatagacatc agtaatgtgt ctgtaattgc
    32941 agattatttt attatcgcgg gaggcaataa ctccagccag atccaggcac tgtgtgataa
    33001 tgtagaggaa aagctgggaa gagccggctt ccctgccaga cagaccgaag gatatgaaac
    33061 cgccaactgg gtactgttag actttggcga tgtgatcgta catgtctttg acaaggaaaa
    33121 ccgtctgctc tatgatctgg aacgtatctg gagagatggc gtacagattc ccgtagagga
    33181 actttaagct tcgctgtctc ccagatggag aaagaagctg atgagataga gtccgctgat
    33241 tcccaccaaa acatatacaa ttctggaaaa ccaggacatg tcaccgaaca ggaaggctac
    33301 aagatcaaag cggaaaagcc cgatcagccc ccagttcact gcacctatta tagctatggt
    33361 gagagcggtt ccgtccaaat atttattacc cataaaatct ccttatcctg tctgtatgaa
    33421 atttgctgct gcaaaatcca tacagacatt tttttgaagt ttctatttaa tgattgtttc
    33481 atagatagtc ttacccccaa aaataaaaaa tatactactt ttaaataaaa attaagcttt
    33541 gttgatctat tttaatgtca aaaaacatca aattagaaaa taaagtccat tgacaaagga
    33601 aatagaagcg agtaatatac aaaaataaca attatacatt gtatatttgt atacattgtt
    33661 aaaaaggagg aagatattat gaagaaattc aacaaggtgt taagtgttgt tgtagcatct
    33721 gccatggtta tggctatggc tggttgcggc aatacagaca atggttccgc tgcaaccggt
    33781 actaacgcag caactgagag cgcagcagct gacaacggtg cagcatccgg cagtgtattc
    33841 aaaattggcg gtatcggacc taccaccggt ggcgcagcag tttacggact ggctgtacag
    33901 catggtgcag agatcgcagt agaagagatc aatgcagcag gtggtatcaa cggctatcag
    33961 gtagagttca agttccagga tgacgaggca gatgctgaga aatccgtaaa tgcttataat
    34021 gcattaaagg actggggtgc tcagatcatt gatggttcta ccaccagtgg tgcatgtatt
    34081 gcggttaccg acaagaccaa agaagacaat atgttccaga tcactccttc cggatctgca
    34141 gtagaatgtg ttcagtatga taacaacttc cgcgtatgct tctccaatcc taaccagggt
    34201 cttgcatccg cacagtatat tggtgagaat ggtctggctg agaagattgc tgttatttat
    34261 gacagctccg atgtatattc ttccggtatt tatgagaagt ttgtaaagga agctgaaaac
    34321 cagccctttg aagtagtagc tgcggaagca tttactgctg ataacaagac ggatttctcc
    34381 gtacagttac aaaaggcaaa ggatgccgga gcagacatgg tattccttcc tatttattat
    34441 caggaatccg cacttatcct tacacaggca gctgctatgg gttatgctcc caagtggttc
    34501 tcatgtgatg gtatggatgg tattctcggt ggtgtagata attttgatgt atctctgacc
    34561 gaaggcgtta tgctgttaac tcctttcgca gctgatgcaa ccgatgatct gacccagtct
    34621 ttcgtaacta agtataagga aaaatacggt gagactccca accagttcgc agcagacagc
    34681 tatgatgcta tctatgtgat caaggctgct atcgagaaga gcggtgttac tcctgatatg
    34741 agtgtttctg atatttgtga tggtatgaag gctgccatga cagagatcac tgtagatggt
    34801 ctgaccggta acggcatgac ctggaccgca gacggtgagg ctaacaaggc tcctaaggct
    34861 gttgtgatcg agaatggtgc ttacaaggct atgtaatttt gctgataaag tttccaaaga
    34921 gggttaccgc agggtagccc tctttgctta atgtcagaga ctttgcaata ctgggagagc
    34981 agttgttttt cccggtatgt tctgttatac tttattttat atgataattt gtgcattcgc
    35041 atgaatggag gtagttcaca tgagttttat cagttatctg atcaacggta tcagccttgg
    35101 aagtgtctat gcgctgatcg cattaggcta taccatggta tatggtattg ccaaaatgct
    35161 gaatttcgca catggtgatg tcattatggt cggtgcattt attacttata caatgtgcag
    35221 taccatggga ctttctcctg tgatcggtgt tctggcagct gttatcgcct gcaccctgtt
    35281 gggaatggct attgaaaaag tagcatacaa gcctttaaga aaggcttctt ctccgttagc
    35341 ggttctgatc accgcaatcg gtgtcagcta tctgctgcag aatgtagctc ttttgatctt
    35401 tggtgcaaat gccaaggcat ttacttctgt agtatccgtt ccggctgtga agctggcaga
    35461 cggacagatc aatattaccg gagagaccat tgttacgatt gttacctgtc tgatcattat
    35521 ggcaggtctg atgcttttta tcaacaagac caaagcagga caggcaatgt tagcagtatc
    35581 cgaggataag ggggctgcac agttgatggg tatcaatgtg gacggaacca ttgcactgac
    35641 ttttgccatc ggatccggac tggccgctgt agcgggagtt ctgttatgtt ccgcatatcc
    35701 ttccctgaca ccgtacaccg gttccatgcc cggtattaaa gctttcgtgg cggctgtatt
    35761 tggcggaatc ggatctatcc ccggtgctct ggttggtggt atattgttgg ggattatcga
    35821 aatctttggt aaagcctata tttcttccca gatggctgat gctattgtat ttgcagtttt
    35881 gatcgttgtg cttctggtaa aacctgccgg actgtttggc aagaatattc aggaaaaagt
    35941 ataagggaag aggcaggtac ataatatgaa gaaattatcg aataagaaaa taaataaaac
    36001 cttccgggat aatcttatta cttacggaat ggtgattgca gcttatgtca tcatgcagat
    36061 tctggtgtct gccggcagtg tgagcagctt gatccaggga ctattggttc cgctgtgtac
    36121 ttatgcaatt ctggcggtat ctctgaacct taccgtaggt attctgggag aactgagcct
    36181 tggacatgca ggatttatgt gtatcggagc attcaccagc gcatttttct ccaaagtaat
    36241 ggagaatagc gcaatgcctt ccggacttcg ctttttcttt gctattctga tcggtgcatt
    36301 ttttgcaggt gtgttcggac tgctgatcgg tatccccgta cttcgtttga agggtgacta
    36361 tctggcaatc gtaacactgg cttttggcga aatcattaag aatctggtaa atattatgtt
    36421 tgtaggccgg gatgagaatg gattccatat ctcctttacc aataccatgg atctgaacat
    36481 gtcagaggac ggagacgtta ttatcaaagg tgcacagggt atcaccggaa cccctcatga
    36541 ttccagcttt accattggta ttgtgttatt gctgatcaca ttgtttattg tattgaacct
    36601 gatccattcc agagacggac gtgccatcat ggcaattcgt gacaatacca ttgcggcgga
    36661 atccgtagga attcatatta caaagtataa actgatggca ttctccattt ctgcggcact
    36721 tgcaggtgta gcaggcgtac tgtatgcgca taatctgtcc acactgacgg cattacccaa
    36781 gaactttgga tataacatgt ctattatgat actggtattc gttgtactgg gtggcatcgg
    36841 taatattcgc ggttccatta ttgcttctgt aatccttacc ctgttaccgg agttactgcg
    36901 tggtctgaac gattatcgta tgctgatcta ttccatcgta ctgatcgtaa tgatgctgtt
    36961 caactgggct ccgaagtgta ttgaatggag agaaaaagtg gcagccagat tcaagaagtc
    37021 tgtgaaaaag gaggctgaat aatcatggca ttattagagg caaaaaattt aggcatttct
    37081 ttcggcggac ttcgtgcggt agatgatttc aatattaaga ttgaaaaagg ttccctgtac
    37141 ggactgatcg gtcccaacgg tgcaggtaag accaccgtat tcaacctttt gacaggtgta
    37201 tataagccca cagagggtat tattacgctg gatggaaagg atgttaccgg taaaaagacc
    37261 atcgaaatca atcaggcagg aattgcaaga accttccaga atattcgact gttcaaggat
    37321 ctgtctgtga tagacaatgt aaaagtaggt ctgcataatc atatgagcta cagcacggtt
    37381 accggtattt tacgtctgcc gaaatattat aaagtagaaa agcagatgac ggaggaggcc
    37441 atggagcttc tgaaggtatt cggattggaa gaggaagcgg aaacactggc ggacaatctt
    37501 ccctatggta agcagagaaa gttggagatt gcgagagcac tggctaccaa gcccaagctc
    37561 cttctgttag atgagcctgc tgcaggtatg aaccctaacg agacgctgga actgatggag
    37621 accatccgtt ttgtcagaga gcattttgat atgacgatcc ttctgatcga gcatgatatg
    37681 aaactggtag gcggaatctg tgaggaactt acggttctga actttggccg tgtcctgtgt
    37741 cagggcaaga ccagtgatgt gctgaacaat ccggaagtca tcaaggctta tctgggtgaa
    37801 taggaggaca acagctatgg caatgttaga agtaaatgat attaaagtat attacggcat
    37861 gatacaggcc ataaagggtg tgtcctttca cgtagatgag ggagaggtca ttgcactgat
    37921 cggagccaac ggcgccggta agacaacgat cctgcacacc gtatccggtc tgctgacacc
    37981 gaaggaagga accattactt ttgaaggtca ggatctggtc aagattcccg gacataagat
    38041 cgtatccatg ggtatggcgc atgttccgga gggaagacgt gttttcgcac agctttccgt
    38101 attacagaat ctgatgatgg gtgcttacac cagaaaagat aaagaagaga tcgcacagac
    38161 tctggagacc gtatttgacc gctttccccg tttgaaggaa cgtcagaatc agatggcggg
    38221 taccttgagt ggtggtgagc agcagatgct ggccatgggt agagcattaa tgtctcatcc
    38281 caagattatt ttaatggatg agccgtccat gggactgtcc cctatttttg taaatgagat
    38341 ttttgatatt atccaggaag taagcaagag cggtactaca gttctgctgg tagagcagaa
    38401 tgcgaagaag gcattgtcca tcgcagacag agcttatgta ttggagaccg gtaagattgt
    38461 tctggaaggc aaggcaagcg accttctgaa taatgattcc attaagaagg catatctggg
    38521 tgaataactc ttgatttaag tcgctgcggg tggaagcagc agagacagaa aggtatggtg
    38581 atcgaaatga ataatgtagt cattaccatt gcaagacagt atggaagcgg cgggcgaacc
    38641 atcggtgaga tgttggcgaa gaagctgaat gttcattact atgacaagga gctgatgaag
    38701 ctggcaagtg atgacagtgg catcaatgaa gcgctgttcg taaatgcaga tgaaaaagtg
    38761 aagagcacga agctgttcca tattgcgaaa aaggagtatc acggggaact gattcctccg
    38821 gaaagtgatg attttacctc cacggacaat ctgtttaact atcaggcgaa gatcatccgg
    38881 gatctggcaa aggaggaatc ctgtgtcatt atcggacggt gtgcggatta tgtgttgaag
    38941 gattatccga atgtcctcag cgtatttatt catgccccga aggaatattg tctggaaggc
    39001 gcagcgaaga agcatagcat gagcccgaag gaattggagc gctttatcaa taagacggat
    39061 aagcaccgtg cggagtacta taagtatcat actggacggg aatggacaga tgcaagaaat
    39121 tacgatctgt gtctggacag ctccaagctg ggatatgaaa gatgcgtgga tgagatcatt
    39181 tcttatatga aagtaagatt cccggaggac taaatgtaaa atatagttaa aaacagcctg
    39241 cagcaaatca tgctgcaggc tgttttgctt atctgtaaaa gcggaatacg ccgaacacgg
    39301 tgccaaggat ctgg
//
LOCUS       DAWEJW010000012        38177 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_480_length_38177_cov_60.546718, whole genome shotgun sequence.
ACCESSION   DAWEJW010000012 DAWEJW010000000
VERSION     DAWEJW010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 38177)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 38177)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..38177
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_480_length_38177_cov_60.546718"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         9149 a         9650 c         7317 g        12061 t
ORIGIN      
        1 gcaacactga gtttatttta tactatttgt gggggagatg caagggaaaa ttattcataa
       61 agcgaatcca gcgtaagcag caatacatcc tcgcctgata atgtttcaaa ccaatccgtg
      121 tatccggata gagaaaagaa aatatatttt gagatcttat atttagcggt aataagtttt
      181 cctcttcgaa ttaaagtatc atatataccc ttatcaatct tttcattctt gaatttacat
      241 tctccgatca cagccatttt gtctatatca gacaatgcaa caacatcaat atctgcagat
      301 tgcgctctta tatccccatt tttgtctgca acgttctcca caccccacca ggtccctaca
      361 gaagtaataa agcaaccata ctcgccggtt attcccttca ttagtatata atatctgcac
      421 atttcttcaa acacggaacc cataaaagaa tgtagtaccg gcttcactgc cttttcataa
      481 taaatttccc cctgtcccat ttcaattaca ctggtagctt tcggaataaa ctcataccag
      541 aacttgaaca tatagtcctt taaaacatac tgggtcttct ttttgttctt ctcctccgtg
      601 atacatttct tcttttcaac caaacccaca tttatcagtt tctctagtga atataatacc
      661 gttggttctt tttccccaat ttttcccgca attgtattca gggtattctc accagatgca
      721 atctgctcaa caatattgtt tacaaccgct atatctgaga attcctgggt caaaagattc
      781 cttgtttcat catatagata tccgtccgtt ctgaagaaca gtcttataat attctcatca
      841 atgctcttcc gcggatcaat cagcgaaaga tactttgcaa caccaccggt aattccataa
      901 cagattgcct tatcctcatt agaatactcc ggaacaaatt ttgctgcatc caggtaatta
      961 aatgcctcca gtttgatctg ggaatcccgt cttccaaaca agggactctt ttcactgagt
     1021 actttctgtt ccataaagct gggcgcagaa ccacataaaa tcactttaag gcttttatca
     1081 ttccaaaccg tatcaatgta tttctgcaag atagataaaa gcgcttcatc tctctccgcc
     1141 caatatggca gctcatctat cacaagcaca agcttctcgt ctccaatatt tttatcaata
     1201 aaatcaaaaa cagcctccgt agtatgaaag ctgatattct ccaccccggg cataagcaga
     1261 tcaactacct gctttgaaaa cagttccaga tttctatttt ttcctacttt tgtggctgta
     1321 taaaaaatag ttttcttatc cttaacgaat tccgtaataa gagtagactt tccaatgcgt
     1381 ctacgaccat atatcaccgt cataccaatt ccatcttttt catagaattc ctttaaagat
     1441 gccagttctc tttctctgcc aataaacata gaatccacct ccgtcttcat taaaatcatc
     1501 tttattcaaa ttgattttaa taaaattctt ctgaataaaa ttcaagatga aatcttatgt
     1561 tttgcaattt ttttctattt tctaaatgct caaaatatct gttgaatttt ttatcccatt
     1621 ctcgcaaaaa tgttgtatac tagtaatcat gaaagccccc tcctggattt ggtctagtca
     1681 actttaatcc taacacagga tcccggtgct ttctttattt ttctaaaaac tgtggtgatt
     1741 caccaaaata tttcaaataa ataagatttg aaatatctca gaattattaa ctgctaattt
     1801 aaagtaacac actttatcaa tttcttcttt gcctattggc aaacctttcc gtctttgtta
     1861 caataatctc agataagaat tacttcgtaa aactacttaa gtataatatt atttttcata
     1921 gtacaaacaa caaaaagaga ggtgtcactt atgttatcat ttaatgattc ctatttttta
     1981 ggagaaaccc gggataattt ttatatcgaa cccatgatga aatgtgtatg ggcagcacag
     2041 ttagaggtca tgtgtgtcat tcaacaaatt tgtgaaaagc ataatattcc ctattttgct
     2101 gactggggga cgttacttgg tgctgttcgc cacaagggct ttattccctg ggatgatgat
     2161 atggatattt gtatgcttcg taaagattat aaccggtttt tgaaagttgc tcccaaagaa
     2221 cttcctgaag aatatcatat taatagtcct tatactaagg aagaatattc ttatgtactt
     2281 tccagggtac taaatgcaca tagtatttcc tatgattctt ctcgtttata ccgctttcat
     2341 cattgtccct atattgtagg cattgatatt ttcccattag acaccttacc tgcggattct
     2401 aacgaggaag cccttttaaa tcaacttgtc gctatcttga tatcggcggt cgggcactat
     2461 gatgattcac cggaaactat cattaaaatg attccagaat tagaaacttt gtgtggtatc
     2521 aagataaaac ctcagcaaag cataaaaaat cagcttcttc gcatagcgga tgctttatgt
     2581 cagtcctata atgattctga cagtaatata attacctatg ttcctgaaaa cactcaaaag
     2641 catattcatt tacaaaaaga ttggtatcaa acctgtgaat atttaccttt tgagaatatt
     2701 attcttcctg ttccaaaaaa ttatgacgct gtacttacca cattatatgg cgactatatg
     2761 actccagtaa agagctctgc cggacatgat tatccattct ataaaaagca aacaactgcc
     2821 atgacagatg cacttacaca aaaaatcatg aatggtgaaa cacctttcca cctttaatcc
     2881 tttaattcta aaacaaatgc cgaagtatat agctaaaaag ctacatactt cggcattttt
     2941 gcgtatcgta aacatttgca aaaagagccg gcaaatagcc gactctttta tggtaaccat
     3001 ttacacttag cctacgatta ctgcagtaag gacagcactc cctgattagc ttgattagac
     3061 tgagccaaca tagcctgacc cgcctgagcc agaatgttgt tgttagaata cttaaccatc
     3121 tcagtggcca tatcggtatc acgaatctga gactctgcag actgagtatt ctcaacaacg
     3181 ttgtctaagt tgttgatagt atgctctaat ctgttctgaa tagcacccaa attagaacgc
     3241 tggttgttca aggtcttaac agccgtttta atcgtgtcta ttgcaccttt agcatctcct
     3301 gtagaaccaa cgtctacagc atcaatacct aaaccagaag catccattga agcaatagta
     3361 agcttaatct gattatcagt cgctgactca gcacctacct gaagatccac atccgtaaat
     3421 gttccatcta acagagcttt ctcattgaaa gtagtcgtag cagccacacg gtcgatctca
     3481 gtaatcaggt tagctacctc cgcctggata taattcttat cctcgttttg attggtacca
     3541 tttgcggact taactgccag ttcattcatt ctctgcagca tatcatgaac ttcgttcaga
     3601 gcaccttcag cggtctgtac tgcggagata ccatcctgtg cgttagcaga agcctgagtc
     3661 agacctctta cctgacgacg cattttctcg gaaattgcaa gtcctgctgc atcatcggct
     3721 gctctgttaa tcttgtaacc ggatgaaagt ttctcagtag atttagcctg agtcttggtg
     3781 gtgagaccta acattctgtt agagttcata gctgtaagat tgtgttgtac taccataata
     3841 tattcctcct tgaattgacg agcgcttccc tgcgctccgt tatttgcttt ggcaatgtct
     3901 gctccgggct cgcacttccc tttactttca tcgccattgt cttccgtatc tccggcgtgc
     3961 gaaactttca aatcggaaga atggttaggt aagcaattgt tttatttgct ttacttgata
     4021 agtatatcgg agaggttttt ggaaacttaa cccctttttg caaaaaatgt tgactttttt
     4081 attttgctaa gatatattat ataaaaagga agcagttttt taccacttgt ttactttgta
     4141 ttcttgagtg gggtacttgc ttcctttttt atttccacca aatcatataa atacaatttt
     4201 ccgtcttgtg tcctatttac taccagcctc cccttgtaaa cattatattc ttctgtcttt
     4261 ctattgttat cataaactgg tagcgcaaat ctgaccgtat aatagttcca gccacctttt
     4321 gcgtctttct tatgtttact cttcttattt tcactatgta ttttattggc ggctgattct
     4381 accagttctc ggattccttg tgctgcattt gccttggctt ttgcccgtgc tccccttata
     4441 tgttctgaat actttgagcc cgtatactca tctgcgaatt tagttcctat ttctattttt
     4501 tctttcgttt gtttattctc aattatttca ccaaggtact tctttaggta ttcttttact
     4561 gcatcccagt caatgtcctg tttattccaa aatataatat ccggtattat aacaatagca
     4621 ttaccttgtt catctcttac tacttttact tcactcatat gttttccttt cagtattact
     4681 ctttccaatc agaaagtatt tttatcattc tattaccttc tcatccagtg aaatcaatgg
     4741 tgatacacct aaaatttttt tgatttgaat taagatttgg tctgtctgcc agaattgata
     4801 ttctgtgaat ttacaatagc atattgttac cgataaatca atcataattt gtgcagtacg
     4861 ctctgactac tctgattggc ctgggtcagc atggattgtc ctgcctgggc attaatcccc
     4921 tcaattgcca aagtaaattc cggtcgatct atccccaaag ctgaagttac ttcggcaaat
     4981 tctcaagcgg aagttacata tgtccctgtc tgatattcag gcagggactt tattttagat
     5041 tatccagacc ggtacgatat aattgtctct gtcaatcgca ctaagtcccg gtttcatgca
     5101 gataactgcg ccctttgcac gaggcgttga tgccttgtcg agtaattcaa atacctttac
     5161 cagctctgtt ccgggatttg cagttttctt aatctcaatc ggattcaata ttccatcatg
     5221 ttccaggaca atatcaatct ccctggcatc tttatcacga taatagtaca aatacggctc
     5281 ttttccacaa ttcaggtagg ttttccgaat ttccgcgacc acgtaatttt ccaaaatggc
     5341 cccgttcata gcaccgcatt ctaacgtttc cgcactgctc catttcgtca ggtaacacac
     5401 cagacccgta tcgtagaaat acagtttggg cgttttcaca aggcgcttca acaaattatt
     5461 agaatacgga tgaagataaa aaataatacc cagcgtttcc agaattgtca gccaatcctt
     5521 cgcctgcgtc tgattgatat ctgcatctct ggcaatctct gctgcattaa ccatctgccc
     5581 acatcttgct gccaccgcag taataaaccg aaggaactta agcgaatcta tggcatccga
     5641 taattctctt acatcccgct caatataagt agacagataa ctgctgtaaa aaagctgact
     5701 gttactgtta ataccgctta cgatcgcagg catggagcct tgataaatcc gttcaaagat
     5761 ccctcttgta tcagcttgct ctctctcttt ctgcctctcg tttaaatcct ccagatctac
     5821 cgtaaacggt ttcatcgttc ctccggatat ctctgcctgt gacagggatg taagtgaaag
     5881 tactgccact cttcctgcca gtgactcctg tactccctgc atcaatttga acacctgtga
     5941 tcccgtaagc caaaatgctc ccggctcatg gtttttatct acatttatct tgatataagt
     6001 aaacagttcc ggtgcatatt gaacctcatc aatcaataca ggtggtttat gcagttgcag
     6061 aaacagttcc ggatcggtct ttgcaatatt tctttcgttt aaatcatcca gtgttacata
     6121 tccccgctcc gttccctcca tcagtttttg gagcattgtc gtctttccta cctgtctcgg
     6181 tcccgttacc aggagtaccg gatattccct ggttacctgt cttactacat tctccagatt
     6241 tcttttgata taactcataa aagtccctct ttcggctaat cttttttatg attatatttt
     6301 agccggatta ttttaaaata tcaagcattt tctggctaat ctatactctg attatatttt
     6361 agccgaaacc acatttttat tctatcacat tgattccgtc ataaaaaagg gaagccgata
     6421 tctctatctg cttcccttta tccgttgtat ttattgttta ttgtcgtaca tctggggcgg
     6481 tacatatccg gcgccgctca tgctcttgta gtagtcaaag gcgctcttgg cactcttgcg
     6541 tctctggccg attccggcac gttctctggc gaaataattc tcgaccagtt tcttgttccg
     6601 ggcttcctgt gcctgtacgg acacgctgag ttctgtcact ttggctacct tggcctgcat
     6661 ttccttgatc tgtgctgcgt agcgctgtct gttgccctcc agctcttttg ccagcttttc
     6721 atacagaatt tcaaagccgt catccaagcg taccacttca tcgatcaggt gctgtttctc
     6781 ctccacggca gcatcgaact gacggatatc caccttctct gcggaaaaaa cttcctcctg
     6841 gcgcttatta tattcctgaa tcttctgtaa tacctccagc ttccgatcca ggctctcgga
     6901 caatatcgtc agctgacttt ccatcagata gcccctttct cacggttgat gcgcatgact
     6961 tccttccagt tatctcttac ggaatgaaga tgcatattga tctcctccag gatttcctta
     7021 tcctttttta cgttggcctc cagcaatctt ttgaacagat aatcatagat attgatgaaa
     7081 tcctgtgcta cgggatattt cgtatccagg gtagactgta ggtatccgat gatattctgt
     7141 accttgcgga tattattatg tgccttctca acatcttttt cttctattgc cacttctgca
     7201 atattgcaga acttaatggc accttcatag agcatcagtg tcagttccgc gggggaagct
     7261 gtcaacactt tactgttatt gtactgcgca taggcattag gtaacgccat atcatgatcc
     7321 tcctagcaaa cttgtcaccg cactggaatt actctgcagc tttgcaagag ccgtctccat
     7381 cttggagaac ttggaatacc atttatcctc gtaatcgttc agcttctctt ccagctcgga
     7441 tattttcgtc ttgtaatcat tatagtccga agtcatcttt ttgtcatcat aaaaacttcc
     7501 ataagaacga tatccgtcta cagatttgga cagatcactc attttggtat ataagttttt
     7561 agacagagaa gagaagaaag cgattaccgt atcgggatca ttactgatca tgctcttcag
     7621 cacatccgat ttaccggagg tattggcatc atccggatca cctgcaatgt gataagcatt
     7681 tttctcattg tccggtgcct caaagtaacc cagggtctca acaccgaaat cactgaggta
     7741 catagtcttg ccgttcacgt ccacgctgcc ggacataata ctcttcaatg cagaactgat
     7801 ggtagacaag ttactatctc tgcgaagcag agcatccttg atcttggtct cgtatttctc
     7861 tacctcagag tcagacatag aatctttctc atcatctgtc aggggttcat accctttggc
     7921 agaatccgca ttgtacagtt tgtccatctc attgatgatg gaattgtact ccttcaggaa
     7981 gttcttcacc atatcataga tgccatctac atcatcttcg gtagtaacag tgatatcctc
     8041 accgtctttc gtctcactga gcgcagtgaa agtcaagccg ttgatttcga agacattgct
     8101 gttgccggtg aattccgcac catttaatgt aatcttggca tcctgaccac tgattttagt
     8161 agctccggta gccgtatagg ttccgttatt gatggatgtt acaacatcgg acgcgtattt
     8221 tgcctgagac agatagctgt tatttacctg gtccttcaat gtctgtgtgg cttctgcagt
     8281 ataagttgca ttaccgtccg catcggtacc ttctgttaca gtaatatgct tctgtatatc
     8341 tgcgatcttt gtatctgcat cggtgatact cttctgtgcg cttttcagag actgggcatc
     8401 cgtcttcttt tcagacaatg tcttaatctc ctcattcagc gtatccagtt ccttctgtgc
     8461 agtatctttt tcatcaccat ctgtcagatt cttgatcttt tcttccagct ctgtcttctc
     8521 tttctgcttt gcttccaact gtttcgtata gttttctaca gtatccaggc tggaatcctt
     8581 atatttttca ttgatctcat cgatcttctt ctgtgcatca ctcttagtgc tgagcagaga
     8641 cttatactgc tccagataac tgcttacttt ggattcaata tcctttgtga tacggccatc
     8701 cttgttaata tttgcaagag tagctgcatc atctccgctt acatagaaag ccgcatactc
     8761 cttatatctg tccagtgttg cctgatcccc cttatcgcca atcagacttg ccttcagtcc
     8821 caatgctgac agcgctgcgt caccggtgct gtccgatgcg gtaatactga agtcattgtc
     8881 tcgcccggat gcggaggcac tgacgaagaa tctctggttc ttcgcatcaa agctggcatt
     8941 gacacctgct tccttgatct tattcagcac atcggaaatg gtactgtcac catttaccgt
     9001 aatatccaca ctctttccac ccgcagtgat attgaaggta ccttctccgg tcactcccag
     9061 ttcgcttaat ttagatgcag cagtcagact tcccccatcg gcagccttga tctgtgcgcc
     9121 ggtgagataa ccggtcttgg ccagcttgtt gatctgcagg gactgtactc ccttgacggc
     9181 attttcaccg gtgatcacgc tgactgcatt actgttggat acttttgtgg tcctcttgct
     9241 gtaagaagac gtaaaacgca tattagcgat atattttgcc tgcagatttt tcagtttagt
     9301 atttaattct ttccaggcat cctgcttcca ctgcagtttg gtctgtgcct ttttggcatc
     9361 atccaccttg gtctgtttgg cagataccag ttccttaatg atagtctctg tgtccatacc
     9421 ggacatcaga ccagacattc tcattgccat aatgtgttcc tcctgattct tacttcttat
     9481 ctcttctcat ctaccaggat accggctaat tcccacactt tggcgatcat gtccagggtc
     9541 ttctcgggag gaagttcctt gatcactttc ttggtatcct tatctatgat cttgatggtt
     9601 acgcggttgg ttgcctcatg aatgccgaag accgcttcgg aatgagacat catgctcttg
     9661 ttcagcttct ctaccgcttt cttgatctgc tcattggtca catgctgttg ctgctcccca
     9721 ttgggatcgg cagaggtatt gctctgctcc ttgccctgag cattctcaac gactgctgtc
     9781 gtctgatcga tcttgtctat tggctttaca ttggcagaat tgtattcaga ggcgttttcc
     9841 tctgtggtca cttttgtagt cgcattcttg acagcggacg tgttctgtgc ctgcactgtc
     9901 atgagactgg aaatcggttc tatggtcata atggtcacct ccgtgtgata aaaagcgcct
     9961 tccgtgggcg cacgaccttg cttctctaag gattgtatcg gtaaattctc ctgaaaaatt
    10021 aataggtgct gcaaaaattt cacgacacca tctattcaat aatgcattcc tgctctaatt
    10081 ttatattttt ctccagcata taccggataa cctcgctgaa ttccttttct tctcctaatt
    10141 tctccagacg ttttttatct gtttctgtca atttttttcc atattgtgaa tattgtttca
    10201 gcgtatccag atccatgcgg aagctttcaa atggaatttc tgttttttta cgtaaatcca
    10261 gaaaaataag gaatcctccg gcatctatgt ccccaaaatg ataatatttt gcagcaggaa
    10321 ttgcatcata aatcttttgg agcagttttc ttcgtaccct gttatggtat cctcccaggt
    10381 aaacaaggag gctgttttcc tcatggtatc tgaaaaaaga agtaaggttt tcaacagtta
    10441 ttaccttctg tattctgctg aaatccgaga aacgaatgtt ttcaatatcc tctcccgaaa
    10501 ttccaattcc ctgctttaaa agggacaaat tcatctcctc cccttccacc agcaggtgca
    10561 catctccctt aaaatacaca aaatcagggg tttgataaat tccatattcc gccaatagtt
    10621 ccactgcatc catttcctta tattcctcat cgaactgccg aaacactctg ataatcctgt
    10681 tttctatctg ttcaaaatat ttagaatcct gaaaatgttg aatggagaac tctctgatat
    10741 aacaaggtgt tttattttgt tctacagata cacatgcacg aagaaatttt tctgtttcct
    10801 gcagattttc caatgttatg tattctttta ccgacttgtg gttttccagt cgttccaaca
    10861 gatatcctac aaaatttacc gtgaccggag catcttcatt cagatatttt tgaagaaaga
    10921 ctctgttttc ttcctccaga ccatgctttg gttttcttcc tgtataccgg taaattacat
    10981 caatttgatc cgcattcatc cgaacctttt gtatgaaatg tccctgctta tggtccttcc
    11041 agattacagt tatcatattt ttattttcca gtgcattcat caaaatatga attttttcat
    11101 attctgatga actctcatca taatatgccg gtatagatgt tctcgtaaaa cgaaactcta
    11161 tctgtatctt tctcctgttc tctccaattg acaacagact gttttcatag gtatccagca
    11221 aatgatttaa aaccattctg tcatactgta ccatttacat acctctctgc ataactgatt
    11281 ttttcatctg tcattacgag taatgtctcc tccacaaagg gactgatata ctgtatttta
    11341 tccggtggtg cagcaatcaa tatctgtagc ggcaaccgcc gcaaaaattc cagaactccg
    11401 cctattcttt catcatccat attattaaac gcttcatcaa aaagcacaag tcccactgcc
    11461 tctccgccaa tattgttctt atacagctga acaaaagatg ccgctacggt gacgtaaaaa
    11521 ggtgtctgtg tctcaccacc gctcttttcc tcacacacct ttgaatataa ggaatagttt
    11581 ccatcgctgt gtataatgcg tatatcataa tccatataag tacgataatc cgtaaattca
    11641 tccagtgttt ttgtactatt gtcattatca agtgccagcc gttcgaacag ttcttctatc
    11701 actaccttat gattctcatg aaacagtcca ctgaaaatag actctccctg catcgcatta
    11761 aaatcatcca tgatcatttc ataatattga cggtatttct tactcggcat atacaggaat
    11821 tcatactttt cattgctgaa cgtaatatca ttcaatgcct tgtttaattc cttaaattca
    11881 gactgggctg tcttcatatt ttcctgtagc ttagacaaaa actgctctct gaattcttct
    11941 tccgctgcgc ttctggccgt ctccacctta tcctcatact gcagcagttc tgaatttttc
    12001 agtttcaaat actccgcttc aaaatcccga tatccttcca tcgaagcagg tgcaccaaaa
    12061 tcatgcgctg ttttatattc cctcattacc tctttcatag agtccgttgc cttatcaatc
    12121 agtgtctggt ttgcttttct ccttcgcaaa taattatctc tgaactgtgc ataagactta
    12181 ccctctgtct gtttctcata ttcctgtctc catattggca ataaatctgc atcgtctgcc
    12241 ccaagttttt cataaaatag tgctttttct acatgatttg cctgattctc tgcaattttc
    12301 tgttttgtca ggctgattct ttcctccgtc ttaccggcag ttctgtctaa attactcctt
    12361 tttaccgtca gttcctctaa cattttttcc aaggtagaca attgcagctg tttcatgatc
    12421 atattggaat ttttcttcaa tgtagcaatg ttttctttgc atttgtcaat ttccagagaa
    12481 acctgccgca aatcaccaat aacgtccaaa cggtacttaa tatctgtatc ctgatccgta
    12541 ttcagtggct ccagtacata ttccagttgt ttcagcctgc ttttcaagtc cgccagctgt
    12601 tgcttcaatt cctcccgctt ctgttttgcc tgttccagtt gtacctttat ggcattctgt
    12661 ccaatgtatg gtgtttcaaa aacggatggt ttaatagcac tcgctacgtt gttctgatac
    12721 ttcatgcact ctcttgtgat tgagatccta taagttttta gttcccgata atgctcacac
    12781 atatgaactt ttccgagaat catattgaca taacgttttg cgtatttatt tttggaagac
    12841 actacggtag ccagagatcc ttccggtgcg gtatcatatt cttccagatt ctgtgtgtta
    12901 ataaggccta ctccatatgc ttttttctct tttcgaagcc tgtcataaat tcccaatgca
    12961 atatcaaaat tttcaggctc caccagaata taaaatctct gcgtgtttaa ataaccttcc
    13021 acagcatttc tccaggatcc atctgtaatc tccaacattt cacataaaat acgcggttcc
    13081 ggagtccgtc cgatacgttg aaactcttcc ttaatttcca ttcttacacg ttctacatct
    13141 tcagaatacg aaaatttttt cttcattaac tgttcaattt tcttgtccag ttcctgcttt
    13201 tcatcgctga tttcggcttc ctttgctatc gtctctgctt ttttctgtag cactttattt
    13261 cgcattagat ttttgtagga aatagcctgc aaaagtgtgt cctttgtctg cgccagattc
    13321 atatcattgt ctggcatgca caagtttcga taataaagtt ttatagatgt gtccacatcc
    13381 tttacttcca gcaatttttt caactgcatt ctggcagagt ccatgctttc ctgaaggctt
    13441 tcccgctcct tcagcaaatc tgtctttctg ttctccaata actgcagtcg cttctcctgt
    13501 tcgtcttttg ccagaaaatc tttatctgtt gcaagttccg cacgcaaatc atctctgatc
    13561 tgttgttttt ccatctgttc ttttttcaca gtctggatct gaccgttttg ttcttccaac
    13621 cgcaaagtat ccgtctgtat ctcagcctgc atcttttcta tttcttccag gataagatcc
    13681 gcttctactc tcgcaagata atatccatat tgcttatcct tctgtaatcc atccgctgcc
    13741 tgggcatgta aggaattgat tttttccaac ctctcgatac gaatttttac gttttccagt
    13801 gtacgctgta aatcttgata agtccggaca ttttcacgta atacatcaat attgacttcc
    13861 ttctcatcca atacatagga atacacaaaa tctttaatat catcaatcgg tcgaaaagcc
    13921 agagccttgg gaatcagccg gaaaaattta tcttcgattc gtccaaagcg gtttttgatc
    13981 attgatcttg cttctttatc tgtagtacac cattgtttta ttttagaact attcattgtc
    14041 cgaaactgat tgatagactt tggcgctttt ccttgaaaaa acatagagtc ctcgaatcgt
    14101 actccgtcca tcaaatatct tgccgttttt tcctgtccct ccgaagcaga gtctaccacg
    14161 gcaccgatca caaaatattt atcctttttc tcttcataaa attccagtgc aacatgtgca
    14221 ctgatctctc ctgtccgttc ataaggttta ttttctcttc cggtcttaca gcgaatatac
    14281 ccggtcagtt ttcttttccc gttttcatga gctgccttat taaaataatt ggttgaacaa
    14341 ataatcacga actgaatagc gtccaaaaga gtggattttc cagctccgtt ttctccggaa
    14401 atcagtgtag atttttcaag atctattgtt tcattaacaa agcgatgcca gttaatcaat
    14461 ttcactctcg tcagtttctt cactgttttt tcctcctctt ttgtaaattt ccagcttttc
    14521 ataagctgcc ttaatatctt ccacgcgaac agccatcaga atagagtcaa agataataat
    14581 ccgggattct tcctccgtaa ggctggtatc caatatctct atgatctgaa accttttcaa
    14641 tgtactgatt gcattgcgaa aagttgtctt gtctatcatt ttctctctga tcttcaggct
    14701 taagaatttt tcatgaatat cccccacatt tacaattact tcctctgtag tagaaagttc
    14761 ccttctcttt tcatcatata aaattctgag aattaacaag agaaccgatt caaacagttt
    14821 catattgtag cggttatagt tttggggatt ggttaactga accacaccat attcttcatt
    14881 aatttccaaa cggtagccca atagtgcaaa acattccgca aatttttcct tgtattttgt
    14941 tacaaaataa taatctaatc tggttgtttc atttctttta cataaaaagc aggaacttag
    15001 cagccggtta catatccgtg agaaattatc cctgtctttc tgcaacattc cgtttaaaat
    15061 atccatccta cttttgtacc ttctttcgta ttgtaaaatc cgtaaattgg taaccttcct
    15121 tctgtatggt ctctcttaca ctcactacat acttcatgtt ttttctctga ccgtacaatc
    15181 gaacataaat catcttcacg aaatcatccg tagtatctaa cggtaactcc gaagctaaca
    15241 tttccggtct ttctccaagc tgtttttcta catatccatc tattttctcc gggttcagga
    15301 ctcttgccag cttttcaagc attttccttt tcttctcatt cctgagttca gtgtccggtt
    15361 cctcctgttg gattatttcc ggttcaaatg ttttctttcc ttcaatcgga gtataaaaag
    15421 atttctcatc tataactgct gaactgtata tccggatcag atcatccaaa aattcaatcc
    15481 tgtaaattcc accaagatcc atgttttccc gattgatttt ctcattcatt tcagaaagaa
    15541 tatccttgat ctgccccctg acatcctcgc cgccaatcag ataaaacttg gcacgattga
    15601 tagctgccct ctgatattgc gtatttttac gattgatttc tgccaggatt tcatccatat
    15661 tctgaaaagc atctatgacc tgatgaatat aatgatagac cagttcttct gcttcttccg
    15721 gcgcaatttc ccgaatggaa gccagttccg cacctgcttt ttccacataa gccttactat
    15781 ggcttttact ttccagtctc tctataatct ccggtcggaa tttagagaca ttatctgaag
    15841 tgagcaatcg atgatatgct ttgtctacaa tattttctat gtaatcgttg aataaaacat
    15901 ccataatttc cgccggagtc ttatactttg tcagttcatc aatataatgc ttgattccgg
    15961 aactcaggtt tttcagaccg gtaatcaaca tatctgtatt ttccacaatt gacaaaagaa
    16021 cgattcccgg attgtccgtt gtacctcgca ccaggctata tatagtgtaa atatatccct
    16081 gatattcgat ttgcttacct tctgcaattt ccaacaatgt ctttataatc ttgacggcat
    16141 attccttaaa attaactctt tgtacataac ttttatctgt ctcgatgtct atccatccat
    16201 aattttccag ttttcgcaac atccagtttg ccttatctct tgcatttttt ccctggaatt
    16261 cttcttcatc aaaatccgct gcctgggtct gttcaaaata atattgcagt tcctccacca
    16321 caacatctct gtccactcca aaggaaagct gatgatccat gacggaaaat aacttgcaga
    16381 tacattccca atatactaat ttattcggag atgccaacgg tacaaaaaaa cgttcatcta
    16441 taatatgaaa cacacgtaat ccctctttat ttatgctttg tatggaatct actgtcctaa
    16501 aaactataag tccagactat tttcattcaa cagaaaatca aaaatactca ttgtcaatat
    16561 tccgtcatca tcattccatg gtttaataat atcttttaca atgataatct ttttaaatga
    16621 atcacctaca tttatcaggg atgccttttc ctgcattctc ttctcatcat ccggaagact
    16681 gaatgctgat tgaatataat atcgtttgct tcccttattt gctacgaaat caatttccag
    16741 ttgttttttt tcctgcactc cctctttggt tctgttccgt ttcataacca caccaacatc
    16801 cacatgaaat ccccgaaccc ggagttcatt ataaatgatg ttctccataa tatgagtctc
    16861 ttctgtctgt ctgaatccaa gtctggcatt tctgagacct acgtcttcaa aataatattt
    16921 cagtggagtt ccaatatatt ttcttccttt tacatcatat cgattggcct cgcttacaat
    16981 aaaggcatcc ttaaggtact caatgtagct ccgaatggta ttcaggctga tatctgactg
    17041 aatcacactt ttaaatgtag cttctatttt cgatgcattt gtcagtgaac cgattcctga
    17101 tgccagtata ttaatcaagt cgtttatttc ctgaattttt tctatatgat tacgttcaat
    17161 aatatcttta atgtatgtct cttcaaacaa tccggtaaga tattccattc tctgctcatc
    17221 tgtattcatc gtaatggtta acggaagccc accaaaatta acataagaac tccatccttc
    17281 atatttatca ccatcgaata catccatata ttccttaaaa ctgaccggaa aaacatgtat
    17341 ttcatctcca cgccctctga actcggtgat aatatctttt gacaaaaatt tagagttgct
    17401 tcccgttaca taaatatctg cattttttat atgaagaaga gaattcagaa cctcctcgaa
    17461 ctcttccagg agttggactt catcaaggaa aatatagtaa tctttttcat ctgttatccg
    17521 gcttttaata taatccaata ttacataggg atctctatac cttttatttt ccaacatatc
    17581 cagttctatc gcaataatat gatcttccgg aacattttgg gatataagat agtttttaaa
    17641 tatggtaaac aacagatatg atttgccgga acgtcttatt cccgtaataa cctttatcat
    17701 tccattatgc attctatcta ctattctttg cagatataag tctcttttta tttccatgtt
    17761 tttctccact ttgatatttt gagaacattg tgccctcttt tttcaaatta tactattata
    17821 tctaagtcct ttcaatgtat ttttataatt ttgggaaatt gtgccctctt ttttcaatat
    17881 gcgtattcag aaaaaactcc ggattgcata ctccggagtc tgtgtcattt ccattatttt
    17941 ctttgtttta ccccaacagg ttcttcagtg ctgccatacc ttcggcatcc acagcggcct
    18001 tgttggcctc ctggatctgg aggtagactt ccttgcggta gatgggcact tccttgggtg
    18061 cggtgatgcc gatcttgacc tgatcacctt tgacctccag aacggtgatc tcgatatcgt
    18121 tgttgatgac gattgcttcg ttcttcttac gggacaatgc cagcatctta ctcacccgcc
    18181 tttccggatt tcaggatgtc gtataccgga tattttaccg ggtatttatc tccctcaagg
    18241 atgatctggc aggccttgcg ttcttccacg ttgatcacaa agggaccctg caggttcacc
    18301 gtcatcttcg tcaggtcagc aggcacagtc acagtaacca tgaccagaat gttctccgga
    18361 gtcagattgc ctacactagt gagcagttcg tcatccactt ccggatcata ctgagggttg
    18421 accagcagcg gatccatgac cggcatggcg aatccgggtt cttccagaga ctgtaaatag
    18481 cggattccta cgttgctgcc cttctcctca tcgtggagca tggtaaaatg cttcagttcc
    18541 gggaatccga taataccttt gtcaaaagtg atgatcttgt catccgcaat ctcaacttct
    18601 ccaaaaaatt ttgtattgat cttcataaaa tacctacgcc tttccgtaac ctgttgttct
    18661 agatgaaatt caacagtgtg gtctgcatta ctttaccggt agccatcagt gccgcttcat
    18721 aggtcatttc cgcactcttc agatcaatgg cggtctcggt aacatcgata tcctcgtttt
    18781 cactctttaa ggtctcaaag gtggtcttct ggctcttcat gcggttatcg atcagttcca
    18841 gtttgctgct tctggtaccg cagttggtaa cgcacaggtt ggcatcatcc agatatccct
    18901 ggaacgtagt aatgccgctt tcgaacatct tctggcattt gtcctttgcc agagtcagtg
    18961 ctttttctac tgcttccatc tgcttcgtca gggtctctgc atccgtaccg gtagcagtct
    19021 cctgcagtcc ctcgatcttg gtcttcacat tctccagatc caccacatcc tgcatggcct
    19081 gtaccagatc gtccaccgta cgtccgatac cgtgctggaa gcactcatct gcagtggaat
    19141 tcacctgaat ggtctggttg aagcctacat catattcgat gacctgtttt tcgctcttgc
    19201 cgtcaagata ggacttgtta taatccgtta cttttccgtc tgcatcggta ctggtacatg
    19261 caaaataatg ctcgggtctc agatcaccct tgacccagtg atccttctga taagttacgc
    19321 ggatctcacc ttcatttttt cccgaggtgg aaggatcatc cttgcaggcc atcagtttat
    19381 cataacggtt cctgccgagc aggatctcac cggtatccgg cacatagatc agtgcatcat
    19441 cattttttgc tgcctcggtg taagggtctt ccgtggagga ctttattgtg aacatagtgg
    19501 aatcccatgt cggttctttc attacagctt cgccggttac agggtctttc tcatagaaag
    19561 aaatagacgg ggctgcagcc gggtttgcat tcttgtctct gcagtcatca taagccagct
    19621 ggatacggta cacctcggtg gacttaatat cctgttccgt gatcttgctg ttatttgcat
    19681 tattataatt tgcagcagta atatccagca attttccggt gtcgatcttc gtcagctgtg
    19741 tgatggagct gttgtccagc tgctcggtga tctcatattt cgtattgtat tctttatcaa
    19801 aactcagaga agtatcggta cgatatccgg taaacacata acggcccgca tagtctgcat
    19861 cgccggtgct gtacacttca tctcccaggg ctttcagctg ttccagaatg atctggcggt
    19921 ctgcggattt cagatctccg ttagatgcct tggtgcactg tgtgatcata ttcgtgatga
    19981 tctctgtcag gttcttcagt gcatcctcgg ttacgtccag ccagctctcc gcatccggaa
    20041 tgttcttgct gtaatactgc gtcacctcgg tcacattgct gcgtaaacgc agggcacgga
    20101 tggataccac aggatcatcg gaaggacggc tgaccttctt ctgggtagac atctgggtgc
    20161 tcagcttatc ctgatatact ttgttcgtat tgatattgga cagattatta cgctgcatga
    20221 tcttgttcgt aattctcatt ttctccgtaa ctattcagga ttctaaaaat cagaataact
    20281 ggtacgttat gcttgcatat aagtaacagt tttctgattc gaatggtgac tcgaatagag
    20341 tcaccacaca cgtgagcaaa attagctgtg tagcagcgag aaagcacgga tacgtgcgtt
    20401 tttgcgaaca tatgtgggaa tccgaatagc tgcctccttt ctagtttaga ctccggtctc
    20461 cagaatcagt ctgtcataaa tctccgtcag tgtctggatc atcttggaag ccagattgta
    20521 tccgttctgg tattttacaa gatttacggc ttcttcatcc tcgtccacgc cggagatgga
    20581 gagtctctga ttgtcaatgg taccggatat atccttaaag cttgcataaa aagtattggc
    20641 ccgggatgca ttcaacgcca catctgacaa aatacactgt aaaaactcgg aggcactgtt
    20701 gccgcggaag ctcatcttgc tcttatccgt agccagattc ttcagatcgt tcaacaggtc
    20761 attctgttcc acgccgtcag atgcattttt tcggtttgcc atcaggctgg gatcctgctc
    20821 catggcatcc agaatatcga aattcttcgc tgtcagacga tagtagctgt catcgttgac
    20881 ttttaccgta actttactgt ttttcttctc ctgcttgtca tatcgcttcg ccgcatcatc
    20941 cagcaggaac tgctcaccac cggtagcttt attaccggtg aacatcttca cgccgggatc
    21001 tccgttgcca ctgtaaccgg aagtcagaat atcgttgaat ttctgagaga aggtacggat
    21061 ccactcattc atctggttca tgtaataagg gatgccctga taggacaggt cggtaccgat
    21121 ctccgccttt ttccctacac ggtcattggt aattccccgg ggattcttct cactgtccga
    21181 taaagtaaat gtataggagt aagtggcgtt gccgttcgca tcatattcgc aggtatattc
    21241 ccaggaatca taatagaatt cctgattgcc cagatcgatg ataccgccct gatccgacag
    21301 attacattta ttcaggtcct gcagataagc cttggttact tttacggtta cggtatcgtg
    21361 ggtcttgccg tctgccgtgg tcgtcgtgcc ggtggcggtc acctgtccgg tgaagttctc
    21421 cccgttgttg ccgtcacgca tctggatcag tcctgccaga tctccgccca tggaagcatt
    21481 gtacaggttg aacttctgac cgtctgacca atagatctca tagagaccgt caatatcggt
    21541 ctggtttact ttctcatagg aggttctcgc cacgcattcc agaccgttat aatcactgcc
    21601 gtccaccagc atctgtccgc ctgcgatctt gaccatatat ctgttggctc cagtctcacg
    21661 gttctcgttg ttggcatcga tgatcggagt ctccttgact tccacatcca cgatcttgga
    21721 taattcatcg atcagcaggg ttctgcggtc acgcagctca ttggcctttg ttccagccag
    21781 ttcaatggtg ttgatctgtt tattcaggga ggcgatctca ccggcaaggg aattgatctg
    21841 atccaccttc agtttaatct cctgattgat atccttctgt actttttcca gattacccgc
    21901 cataccattg aaataatctg tcagtgctcc ggcatatccc acgaactggg atttggctgt
    21961 ggcactgttg ggatccttca ggagtgcctg cagtccggtg atcatcagct gatcgaagtt
    22021 cgtcttgaac ccggtgctct taccgtcatc atcaaaatac gtctccaatt gctgcatgta
    22081 atactgtttc atgtcatatt cgccaagcag tgcattgtta tcccaatatc ttccgtcata
    22141 gaattcatcc cgtacacgct caatggccag agtctctaca ccggcaccgg cacagccata
    22201 ggtctggaac acccgcagcg cattggcggc ctgttgtgtc acctgttgtc tgctgtagcc
    22261 ttccgtctgt acattggcga tattattgga agtggtattc atggcagcat tgctggccag
    22321 aagtcccgta tatgcaatat ttaatccaaa aaactgtgag ggcataactt acctcctatt
    22381 tcttacacat tacacaccca gcgtggaaag aatgtgatcc agcatttcac tgatcacgtt
    22441 gatataacgg ctggatgcgt tgtatgcgtt ctggaacttg atcatgttgg acaattcctc
    22501 gtctgtggaa acaccgatga cctgctctct ggcactctgt gtagcctcca cagtggtgac
    22561 ctgattctca taaatactcc ggaatacata gccggaattg gatacctgcg ataccaggtc
    22621 ggtataataa tctacaaaag tagtcttttt ctgtacatta ggattcagcg tgtaggcttc
    22681 ctcggtaaat gccgctttca aagcatctgc ggtctcctta tcctcggaac cgtctgccaa
    22741 acggaatcca agcttggaag gctccttcgt cagcgccgga tttacctgaa gattgccgat
    22801 ggtatacagg ctctcagggc tgtccggatc ttcatccttc ataatccaat actctgttac
    22861 accgtcttct ccgatcacct tctcatagcc atccgttgtg accttggtaa acagttggat
    22921 gggactgcca tcctccatgc gcatatagcc attcggatcc gagacacaat agcggacatt
    22981 ggggcgtgta gtaccatctg caagtttcag atctccggtc tgtactcctg cagccttcgc
    23041 cagaatatcg ttcaccttcg tcaccacatt gtggatcatc tggtcgaact caccctgaat
    23101 attcatgaca acggactggg agacactgtc gtattttcct tccgccagat ctgtataatt
    23161 ggctctgtga tcacctctgg ccagaagcat tgccttcagg ccgccgatat ctgtgttcag
    23221 gtcggaagaa atctccacgg agagatcaaa tacctctgcg ccgtcaatat tataatctct
    23281 ggtgccatca gctcttacgg tgtaggtggc atcctgcggc cagaacggag tgtagaagcc
    23341 ggtggcttca tccgtcttca gagcgatctc ataacaggtt ccgtccttta cgaaatccac
    23401 gccctcgatc tgtacggata cactgccgta tcgatcttcg gaaaaggtca tgttggtcag
    23461 ctctgccagt tcatccagaa tctgatttct cgcatctctc aggtcgttgg catgttcaat
    23521 gccgccactc tcaatggccc ggatctgatc gttcagcgtt aataactggt tgccgtattt
    23581 attgatttta tctacattct gcttaatctg tgtgttcagg ttgttctggt aggagctcaa
    23641 tccgctgtat actgctccgg ctctctgtac gaattccgcc gctctctgca ccagcatgcc
    23701 ctgtgttaca gaactgctgg gatccttgga aagctcctga atagcggtcc agaagtctgt
    23761 catggtggtc tgaaatgcct cgccgttcaa ttctcccagc tggctctcta cctcttccat
    23821 gacttctgtg gacacttcat agaacatact acgtccggat tccttgcggt atgtcttgtc
    23881 caggaaataa tctctgacct gttttacgcg ggaatatgtg acacccagac ctgtctgttt
    23941 gttggaaata gacttagggt ctgtcgataa tgtgacataa gctctgttag actgctgtac
    24001 ctgttgtctg gtatagccgg tggtgtccac attagaaaga ttatgcgctg tggtattcag
    24061 cgcattctga cttgtctgta atccggacga acctatatat aaactaccca ttaatggcat
    24121 aaactttcac ctctgtatca ggggatcctc agatccccgg gatccggtct tactgcttgg
    24181 catcgaatcc atggtcaatg acccccatgg tattgcctgc attgtaagct ccccggttgt
    24241 aattcgctgt ctcgggtgca gccttggatg cctggaggag attcatctcg aattccacca
    24301 tctccagcgc gctgtttaac agttcactgt tctgctcgtt cacgcgcttc agtccccgga
    24361 ccgctgcctg taatctgtcg tgggaatccg ccagtgcctg ctgttccgca ggccgtgcag
    24421 ataacatagt gatcagattc ggcaatttca agtcgttaac atccctgttc aatacatcgg
    24481 ctatgtccgc agtcactccc gctctctgct tgtccagatt gtggatcctg ctgaccagct
    24541 cctgctcctc gtccgtgatc ttttgtaact ctgcaagatc accgcctgca ataatcggtg
    24601 tcttgcgctg tgatagctga agcagagctt cgtactccgt actctctctg tccagtacat
    24661 ctatcaggtt ttccattaaa ctagccaagg aactacctca tttcttcctg cttttgaagc
    24721 agcttctccg caaagcttgc attatccact ccataagtac cgttctggat tcttgcttta
    24781 atgggagccg ttaactcttc cctgatgtca ggtgctgctg ccactgctgc cttagcggtc
    24841 tggaaatcct tgccctggct gctgatctgc agtctgtccg tgtgtgacgc tgtctgagtc
    24901 ttctgagtcc tgctgacctt ctgtgactga tacagctgct gtacctgtgt atacgcttca
    24961 atacgcatat cgactcctcc ttcccgaatg tcttttgatg tttcacaata tgtatcggac
    25021 ggatttacaa ttactttagc cctcatggaa aaaaagtaac gatccggacc gcctgcaggg
    25081 gaacgtccga atcgtcacaa aatctgtcaa tatgcatttt cgttgcggat ctcatcgtct
    25141 acccgccgta cccggtaggt cttgtctccc accgtcacgg cggtcaccgt atcgtcgtgg
    25201 gcatacaatc cttcttccac ataagaaaat ttttccggta tccttccctc ttccaggatc
    25261 tggatcaaag cctccaccct ggggccatgc agtggattgc attctccgat gcaggtgatc
    25321 tcatccttca gcacgcagtc catggattct gtgctgacac catcaaaaga caatatcata
    25381 atatctcctg ccgccagatt gctgccgacc ctttttcccg ccgcccggat cgcttccatg
    25441 gcgccaaagg cctcattgtc gttttcgcaa tagaccacat tgatattgtc gtattttttc
    25501 aggaaagctt ccatgacttc cttccccttg gcctgggtaa attctcccga tacctggtct
    25561 aacagatccc aaccgtattt tgccgcgccc tgctccaatc ctctggtacg tccgatctgg
    25621 gcggaagctc cgatatttcc ctgaatattt acaatatgaa gatcctccgg tgcaattccc
    25681 ttgctgatgg aaaaggaatt cagccactcc gacattttca ggccttccag ttcaaagtcc
    25741 gaacccacga agcaggtgaa cagactgtca tcctccacat ccaccttacg gtccacgatg
    25801 accaccggga tcccggcatc ctttgcttcc ctgagcaccg tgtcccatcc ggtctccgtc
    25861 accggtgcca gcacgatgta gtccacctcc tgctggataa aggttcggat ggcccggatc
    25921 tggttggcct gcttctgctg accatcctcg aagatcaggt cgtacccggc ctcttttgta
    25981 aaggtacttt tcatggactc cgtgttggca ctgcgccagt cggattccgc tcccacctgg
    26041 gaaaatccca ccacaatacg gtctgtctgt gtatcctctg gtgttgcaat atcctccgca
    26101 gtgctttgtc tgtcgccgca tcctgcaagc aggattccca gccccagcag cagacccgcc
    26161 atccatcgtc ttcctgttct gcatttcttc atatctgtaa tgtcatcctg ttctgtcata
    26221 cttcccgccc ggaaatcgct ctgtcattct ctcattttcc ggcaggttct ttctctgtat
    26281 gaaaatacgc cacagagctc tccagttccc cggagatctg tttcatttta tcacagtttg
    26341 cgcccactgt ctgtatctgt gtcaggattt cctcaatgtt tgcattcacc tgctggttgt
    26401 tggcagcatt ttcctcactg atagcactga ggctgccgat attgcccaga atatcctctt
    26461 tctgctgtga cagacgtccg gtctctccgg cgatcttgcg gatctcttcc gcagaatgtc
    26521 tgatactttg ctctaactgc tgatactgct ctctggttgc aataatgtga tcctgttcct
    26581 cccggatgat attctttacc acatccgcct gttccacagt ctcaccggat ttctccataa
    26641 tgccctgggc cagatttttg atctgctcgg caccggcagc gctctgctcc gacagtttgc
    26701 ggatctcttc cgccaccacg gcaaagcctc tgcccgcctc tccggctctg gcggcctcga
    26761 tggaggcatt cagacttaac agattggtct gtccggaaat ggagaagatc agagctaccg
    26821 cctcatcgat cttttcgata taatcgttgg tctcatgaat cttcgctgta atggtattca
    26881 cggattccac agaacgattg ctgccggtca tgatcttttc catgccttcc cccgcctttt
    26941 tgctgttgtc ctgtatttct tccgaatcct tctgcagctg tccggcactg acactgatgt
    27001 cattgaccgc ctctccgatc tcatccatac ggtcactgat attctgcaca ttttccgcca
    27061 tttcctccgc tgcggaagac agctgctcca tcgcctgact gatctgtcca gcctgttctg
    27121 tggtggcatc gcttccctct gccacctgcc gtacactgtt tagaagttct ccggatacct
    27181 cctgtacacc gccgatgacc tgtgccagat tccgctgcat ctgtgaggta ttctgtaaca
    27241 gattacggat ctcccggaca atactgtcgg attcttttct ggcactcaga tcaccttccg
    27301 ccagggcatc caccctgctg ccggtctctt tcatcttgcc cgacagtgct ctgctcagga
    27361 aaaagcagat aacaccaaac aggatcacca tgcccactgc gcagaccatg aatacaacct
    27421 cgatctccca cagggcatcg gtgatctcat ttttccgttc cgccgcaaaa gccactgcag
    27481 tgatctgtcc gttattcata atcgggatat aataaccata ataagtattt ccctcaatca
    27541 ctacattttt atcataatat ccctccgtcc caagatcctg atccatgggg atttcacgga
    27601 tacgatagtc attttcattt ttaatggatg tggtagccgg aatgccgtct gcaagaatgg
    27661 ccatctccag gccttcttcc ttcaggctgt ccaggtactc ataataaccg ctggcattca
    27721 ttgccgtgat ctcattttcc ttacagtatt ttgccagatt atccgcagtt acatgcagtg
    27781 tggtctgcac actgccttcc agattcgttc tggtcagacg taaagatgtg gtactgagga
    27841 tcactgttgc caaaactacc ggtgccagtg ccagcagcat caacaacagg aacaggctgc
    27901 atccctgctt tttcagagtt ttctgctttt ttaccttcgt ttttttcata acttctaccc
    27961 cctgatattc ggtggattcc ttttactagc ggaaaaagag tgtgactgca caccagccac
    28021 gctctttccg ctcctcccta ttttcagctg cttttatttg ttctgcagca ttttcttttt
    28081 tcttgcgatg accacactct gtactaccag gaacagacac agcatagctg caatggtgat
    28141 accggtccac catgcctgat ccagacctgc actggatacg atattctgga tggtgctcag
    28201 ggacaataca ccgaagaaag ttccgatgat attgccgaca ccaccagtca gcatcgtacc
    28261 gccgatgatg gaggatgcaa tggcgttcat ctccatgccg ctggcatgag aagcagatcc
    28321 gcttcctaca tgcaggaagt acacataacc gccgatacct gccaacagtc cgcagagcag
    28381 atgggacatg aacttggttc tcttcacatt gatacctaac atcagcgcgc tctgattgtt
    28441 accgcctacc gcatagaagc tgcggcccat cttggtccag cgaagcatga taaacatcag
    28501 aatgactact aaaagcgcca caatcacacc gatctccaca taagcattga cgtaattgcc
    28561 cagtttgttc acggagccca ttcctggaac gatcacacgg gtttccttca aagcagtgaa
    28621 gctttcgttg gccacgttga aaggtttggt atttacgatc gttgtcatac ctctggcgaa
    28681 gaacataccc gccagggtca cgataaaggg ctggatatcc aggtatgcta ccaggaagcc
    28741 ctgtacgata ccgaaagcaa ggccaatggc tactgccagt aacatggata caaatacatt
    28801 accgccgccg aagtccagat atacggcgca gctcatgctg accagtgcca ccacaccgcc
    28861 gacggaaata tcgattccac cggtaatcat gactaaactc atgccgcagg acagaatgat
    28921 cagtgctgcg ttagcgttca gaatgttgaa aaatgtctga ggttttaaga agccttttcc
    28981 ctggaacacg atggctccga tatacattac gaagaatact acgatcgtga tggtcagcag
    29041 cagattggta tctgtcatat tttttaactt cttacccata tcagccaacc tcctttacag
    29101 ccttgccctg cttcagcttc ttgccgaaag cggacagcca ttctcttacc ttgggagtac
    29161 ttaagactac caacaggata accactaccg ccttgtaagc aggaagtgca tcggactgta
    29221 cgtcgaattt gtacaaggtg gtggtcagga actggatgac ataagcaccc agaatggaag
    29281 ctgccatgtt gaacttacca ccgcttaaag agttacctcc cagagctact gccaggatgg
    29341 catccatctc gatatccttt gcaataacgg agtagttgat ggaggacaga cggcttactt
    29401 tgatcagtgc tgctaccgct acacacagtc ccaggatcac aaaggtcagg aacttgataa
    29461 atgtgggatt cagtccgttt aacttggagg aattctcatt gataccaaca gactgggtgt
    29521 aaagtcccag tgttgtgaac ttcaatacca ggaagatcac cagtacacag accgctgcaa
    29581 tgaagaacgg tgtgggaatg ggaattcccg gaataaatcc accgaaatag ctgaaggtcg
    29641 gatcacttac aataggaagc tcgttgttgt taatccatgc tgcaatggaa cgtcctgccg
    29701 tatagaggat cagggtcgct accatgggct ggatcttgaa atatgctacc agggcaccgt
    29761 taaaggcacc gaagagcatg gctaccacac aggctaccag aaatgctacg ataatggggg
    29821 cctgcagggt atcgggtctg gaattcgtac cacacagtac tcgtaagatc acgctgcctg
    29881 cgatggcaat ggcagcgccc acgctgatat cctgtccgcc ggaagctgca gtgaccagtg
    29941 tcatgccaat ggctaaaata gccagttcgg aaccgtagtc caggatcgtc atcaggtaac
    30001 cggacaatac cggatctcct gcgctgttat atcccagtgt gatcttgaag aaactggggt
    30061 ctacaatcag attgaatact gccaggatta tcagggctgc aatgggaatg aaaatctgct
    30121 gccggaacaa tccgctcagg aaatttttat tacctttttc tactttcttt tcattactca
    30181 tgcttacttt cacctccggc aatcgtactc ataatcttgg tctgggtcag atcttctccg
    30241 gacaattctc ctaccacctt gcggtctctc atgactacca gtcgggagca ggtacggagc
    30301 atctcgtcgg tctcggaaga gatgaaggtc acgctcatgc cttcacttgc cagcttcagt
    30361 accagcttct ggatatccac cttggtaccg acatcgatac ctctggtggg ctcatccaga
    30421 atcagatatt ccggatgggt cagaagccat ctggctaaga ttaccttctg ctgattaccg
    30481 ccggacagcg acttgatggg tgtctcggcc gatgccgtct tgatatccag cagtttgatg
    30541 tattcgtctg caaatttatt agcttccgcc ttggaaatag ggtggaagaa tccacgaagt
    30601 acctgtaatg ccaggataat attctctctt acggacaagt cacctacgat accgtcacgc
    30661 ttacgatcct ccggcagata gccgattcca caacgcatag catccagagg cttattgatc
    30721 ttgacctgct tgccgttctt tttcaccgta ccgccgatga cacggtcagc accgaaaata
    30781 gcgcggacgc actcacttcg gccagatccc agcagtcccg taaatccatt gacctcgccc
    30841 ttcttaatat agaagtcaaa gggtttgatt ccggcatcgc tgcacagatc tttggtctca
    30901 aataagggtt ctccgttctc cgtcttctgt tccgtgggct gctcgccttt aatatcggac
    30961 aggtcatcca gttccttacc caacattttg gataccagtt gtactctggg cagatccttg
    31021 atggcatatt cacctaccag actgccgtca cgcataacag tgatcttgtc acacacttca
    31081 tatacctgat ccaggaaatg ggttacgaag atgataccca cgcctctggc cttcagatcg
    31141 cgcatcaggc cgaagagctt tgtcacttcc tgctcatcca gagaggaggt cggctcatcc
    31201 aggattagta ctttacaatc catatctaca gctctggcaa tggctaccat ctgctgtacg
    31261 gcgatggaac agcttgccag ctgctgggac gcttccgcgg ggatatccag attctgtaag
    31321 atcttgtctg cgtcctcatt catttttttc cagttctgat agatcttatt ggaacgtcct
    31381 atgtacatgt tctccgccac ggacagattc gggcagagtg taatctcctg atataccgta
    31441 ctgatgccca gcttctgtgc ctcctgcgga gagtgaatgg atacggcttt gtcattgccg
    31501 tcgatgaaga tctgaccttc atctttgctg tagacacctg tcagtacctt gatcagggtt
    31561 gactttccgg caccgttctc tcccatcagc gcatgaatct cacctttgtt cagatggaag
    31621 tccacacctt tcagagcacg tacaccggga aagcttttgt gaatattttt catttccaat
    31681 actgtgttat tgtccaaaag tttcacccct taatctgtaa ctattcagag ccatgtaaat
    31741 atcatgaaat gttcttcgaa tgctggcatt cgtaagaata ttttatgata tttatagggc
    31801 gaagtaacca cattcgcaat aaagtgggct ctgaaagagc cttttgcgaa tggggttctg
    31861 aatagttact cttttgataa aagaagcgcg gagctagcat gcgccgcgct tctggtcgtt
    31921 tacgtcagat tagataccgt agttgtctac atcttcctga gtaatggtgg ttgcatcgaa
    31981 gcctttttca tccatgatga tggtcttctc ggagatggtc tcgccgctct ccagcttctt
    32041 gatgatgtca tcgatgtagg aagcctggaa aggattgcac tgtccatcgt agttccagtt
    32101 gcctgccttc agttcttcca gagcccactt gttgcagtca aagcccataa cgataacgtc
    32161 tttaccaacg ccgtgggaga tattagcctt atccagagct gctacagcac ccttagccat
    32221 atcatcgttc tcagcgtaga ttacgttgaa aggagtacct gcgtcgatta cagactgtac
    32281 gatctgctgt gctttctctg cgttccactc agcggtctgc tgtgttacca gattgaatcc
    32341 gtcgtttgct gctgcgtcat ccagagcacc ggtacgtccc tgctgtgctg cggaacccat
    32401 aacaccctgt aagtggataa tgttgtattc acttaagttc tggctcttta accagtcaac
    32461 tgcggtctgg ccttcctttg ccatatcgga tacgatggat gcctcataca ggctctcatc
    32521 tgcatcaatg gtacggtcga agaggataac tctgatacct gcatcctgtg catccttcaa
    32581 tacggaatcc caacctgcgg tatctgctgc ggacagaagc agatagtcaa cgccatcctg
    32641 aatgaacttc tgtgctgcag tgatctgctc atcgttcttt aagctgtatg cgaaagatgc
    32701 ttcgtagccg ttagcctcgg taaaggtatt cttcatatcc ttatcgtttg cggtacggta
    32761 accggactcg ttggggtcgt tattgatgat gccgactttg atcatatctc cggaggagct
    32821 ggagctggtc gttcctgcgc ttgcactttc ggttgctgcg tttgcacttc cggttgctgc
    32881 gtttgcactt ccggttgctg cgttgctgcc tgcatcagag gagccgcatg ctacaaggct
    32941 gaataccatg gttgctgcca ttactgctgc taaaattttc ttcttcattg tgtaacctcc
    33001 caatttgttt ttgtttccca aagaactatt tctttgatga tcttattgta aagcggatcc
    33061 ggggaaaaaa ccatgaagat acctcacgga aaagttgaaa tttttatggt aaaaaaaacc
    33121 accggcgtaa aaagttgatt tttttacgat agtggttcat ttttttaata ctgcggaagc
    33181 cggaccgtga cacaggtgcc ctccccgtag gtactggtaa tggtgatacc atagttttcg
    33241 ccgaaattca gacggatacg ttcgttcaca ttgtacaggc cgtagatatc ggtctcccgg
    33301 gccttccggt tgcggatgcc tttcctcacc tgctctagcc gctccggcgt catccccttg
    33361 ccgttgtccg tgatcaacag aatgcaatcc tctccgtcca gttctccttc gatccgtatc
    33421 atgcccttgc cacgtttgtt cttgattccg tggtacagtg cattttccac cagaggctgc
    33481 agcgtgatct tgggaatctg gcaatggttt aattcttccg gaacacagat ctcatattga
    33541 aggatgtcct gataacgcat ctgctggatc tccaggtagc tgcggacgtg ctgcagttct
    33601 tcttttacat ccagaatatc ctgtccctga ttcaatgaga tccggaaaaa gtccgacagg
    33661 ctgcctacca tgctcacgac ctttttctgt tctcccgcct ccgccagcca tacaatggca
    33721 tccagcgtat tatataaaaa gtgcggattg atctgggact gcagcagttc aaattccgcc
    33781 ttgcgcaacc ggatctgctc cgtcttcacc tgctctaaca gttcgttgat cttgtcgatc
    33841 atggtattca gtgaatcgct gagcacctgg gcctccacgc cccccgtcac gtcggagcgc
    33901 accgtcaggt caccttttgc cacctgatcc gtcacctggg taaggcgtct tatggggctc
    33961 gtgatactta acggtatata ataggataag aataaaaaca gaaccaggat cagtgtaaag
    34021 gcaatgatgg agaaacggat catattcacg aagaaatcct gatattgctg cctgctctcc
    34081 tgaatccccc ggatctcgta atagatgtac tgggacatgg tatcacccac cagagacgtc
    34141 acaatctgca catcattttc ccagatttcc atgttgtcct cgtagcggtt gccttcccgg
    34201 atattgtcct cgatcctgcc gatataggtc tccagattgt tcagatattt tttgacactg
    34261 gtcagtcgtt tcaggttctc ctgagattcc gtcagttctt ccagaccggc cacgatgtca
    34321 tctgcctcca ccagcatatc atgcagactg gactcttcca gcgtcttgtt ccctacgatc
    34381 agcagataag tctcataatc gaaatccttc tggaaatcca ggctgaactg actggccatg
    34441 acggaggaat tgatcatatc ctcgtatctg cggttattgt tccataaatt atagaagcaa
    34501 aagatcagga acaatacaaa aggcaccaga aggcaaagat aggaatatct tatctttgcc
    34561 accagtgtcg aatgttcata caattctttt atcttttttc tcataggaat cccccttatc
    34621 catggccacg gctaattggt gccgccccgg tactgggtag gggtcattcc ctgggtcttc
    34681 ttgaacagat aggagaaata gtgtggatcc ttgtagccca cctcctggct gatcaggctg
    34741 ctgcgcttgc cggtgcagcg caagagttcc tttgccttgt tcatacgata gtctgtcaga
    34801 tatttgataa agctctgtcc ggtctgctgg ctgaagatca tgctcagatg gtttggtgag
    34861 aaattcacgt gggatgccag cagattcagg gaaagttcct cgtctgcata attctcatca
    34921 atgtatttca tcacctgttc caccacatca tcataccggc tgctggcagc cttatctctg
    34981 accgccagtg ctttctgcag aattctggta atgtaatgga tcgcactatc gctgtcctgc
    35041 agggtcttct cgtcctgatt gggtgtttcc agttcctgcg tatccgtctg caggctttcc
    35101 agaaaatctg ccacacagaa atagacatcc atggtaatat actgcctgaa aatattggac
    35161 tgcagtgcct tgttgctgag atcccggaaa aattcttcca cgaagtagac cacttcctcc
    35221 cggtcaccct gttttaagaa ttcctggatt cttgtgcggt cgatctgctt tggattgact
    35281 tccttgatgt caaaattatc ttccttctgc gcttcctccg tcatatgtcc gctgtccagg
    35341 atccggctgt cctgcaccag atatctgtgg gcaaaagcat gactggcctg ttcaaaagat
    35401 gtcggaagct cccgcagacg gttcaccggc ttgccgatac caccgaaata ccgcacatgc
    35461 tcatagcctt ccataatgtc cttgatcttt tctatgtatt cctgttgcaa cagcaatagc
    35521 tgttcctcgg aatcggcttt taataagaat gcttttcctt ccagattacg atcaaaggtg
    35581 atcaccgtcc tgccgtcttc cagtccccgc agttcctgtt cgatccggat caggctgccg
    35641 gaatactcat cctgcgcatg acgcagagac tgggtcttga acagaataat attgtaccag
    35701 ggtgccgaca gatcgatccc cagaatctct gccgattcca acagttctgc cacggtgctt
    35761 tctccggtga ccagttcctg gaacagtttt ttccgctctc tgagattgtt ttcctccatc
    35821 tcctgcaaat accgctctct gatctgaatg gctctgcgct tctcctcgat cttcgccgac
    35881 aattcatcca attccgctag cagttcctca ccattgatgg gtttggacag atactgggcc
    35941 actcccagct tgatagcttc cttggcatat tcgaattcct ggtaaccgga cagaataatg
    36001 atttctgtgt ccggaagctc cttgcggatc agacggctca gttccaggcc gtccataaac
    36061 ggcatccgga tatcggtgat cacgatatcc ggacgcaatt tctggatcat gggataggcc
    36121 agctctccgt cgccggcctc cccacagaac tcatagccat gagccttcca gtcaatatta
    36181 tttttgattc cttcacggat gacaaattca tcctccacca aaaacacttt taacatttct
    36241 acgataccct tcttctatct gcaggtaact tctacccgca gattctgcct gcagattttt
    36301 atttcgttct ctgcaatact attttctatt atacacgaaa acagattttt cgggtagcat
    36361 attttagcgg tagaatactt ctcccagcat caggtctctc ttcagatcgc ggatcttcgt
    36421 atccttgtcg atgtatacag cctcgatgcc catcatggct gcccagtcac ccatctgctc
    36481 tgcggtcaga tcgtaggtaa atgcggtatg atgtgcacct cctgccagga tccatgcttc
    36541 cgcgccggta tacagatccg gttcgggagt ccagaagttg gtagcggtag gaagcttcgg
    36601 catgggtttc tccactttct tgcagttcac atcgttgatg atcagacgga aacgattgcc
    36661 catgtcgatc agggaagtag cgataccatg accttccttg gaagtaaata ccagtcttgc
    36721 aggatcttct ctgtctccca tggacagggg ctggcacttg atgctgatgg gaccgtccgc
    36781 aatggtgggg cagatctcca gcatatgggc ttccagaata ccttccttgc ccttgatcag
    36841 attgtaggtg taatcctcca gcatggaagt acccttggca tcctttttgc ctgcggtcat
    36901 gatcttcatc agacgtacca tggcggctac cttccagtca ccttctgcgc cgaagccgta
    36961 gcccttctcc atcagtctct gaatggccag tccgggcagt tgcttcaacg cacccagatc
    37021 tccgaagtgg gtaacgatgg cctgataatt cttctcttcc aggaaacgtt caaatcccag
    37081 ttcgatctgt gcctgtacag ccacatgttt tttaaactct tcggggtctc ttccctctaa
    37141 taaaatatta tatttctcgt agtactcgtc taccagtgca ttgacatcgg atacagatac
    37201 ggcatttaca ctctctgcga tctcgtttac agggtaagcg tctacttccc agccaaactt
    37261 gatctgtgct tctaccttat caccttcggt taccgctaca ttgcgcatgt tgtcagccac
    37321 acgcatgaca cggatatggc tgctctccac cacgccgact gcagcacgca tccaggatgc
    37381 gatcttgccc tgtaccacag gatcagacca gtggcctacc acaaccttac gctccatgcg
    37441 catgcgggtc acgatatgac cgtactctct gtcaccgtgg gcagactggt tctcattcat
    37501 gaaatccatg tcgatggtgt catagggcag ttcctcattg aactgagtat ggaaatgcag
    37561 caggggctta cgatactcct gtaatcccag gatccaggat tttgccggag agaaagtatg
    37621 catccaggtg ataacaccgg cacaagtgtc atccagattt gcttcattaa aagtacggcg
    37681 gatcagttcg ttagtgatca gggtcggctt ccataccacc tcataaggta acacaccgga
    37741 agcgttcagg gcatccacga tcttgtgaga atgctccgct acctttgcca ggcactcgtc
    37801 cccatacaag tcctgtgatc ccgtacaaaa ccagaattga tatttttttg tctgtaacat
    37861 aagtaatacc tcctgtagtt gaatacaatg atgtcggggt caaaagcccc cgactttgat
    37921 gcctacggat tgttccgtgc tacgcactcc cacaatccta cccaatattc gcatatcgtg
    37981 ggattatcat cccactttta tgctcatatc ggggcgggtc ctaaggtctt tcacccacct
    38041 atgagcaagc tcatatgggc ttagaccttt tgaccctggc atcatacaat ttcgatatta
    38101 ccatattaca acttgtatgc tcctgctaac aagttgtatt tcctacgaca agctccggct
    38161 ggatgagaat ctttctc
//
LOCUS       DAWEJW010000013        36199 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_518_length_36199_cov_54.423224, whole genome shotgun sequence.
ACCESSION   DAWEJW010000013 DAWEJW010000000
VERSION     DAWEJW010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 36199)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 36199)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36199
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_518_length_36199_cov_54.423224"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3679..3778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5378..5477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20101..20200
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        10629 a         7201 c         9529 g         8540 t
ORIGIN      
        1 gaaagcagat cagaggtctg acacaggctt ccctgaatgc acaggatggt atcagcgcag
       61 tacagaccgc tgaaggcgct ctgaccgaag tacatgatat gctacagaga atgaatgagc
      121 tggcagttaa ggctggaaat ggtactatgt ctgctgatga cagaaactat gtacagaatg
      181 agatcgatca gcttgtaaat gaaatcgacc gtgtatctga aaccacgaaa ttcaacgaga
      241 cttatctgtt aaagggtgac gcaaccgcaa atggcaatca gaagactttt gcggcaggcg
      301 acgtattagg aacggttgat ggagtaactg tagcagcaaa gggtaccacg gaggctacat
      361 tgacggatgg tgctgaaatt actattggaa aagtaaaagt agtatcaagt gataatacta
      421 attttacaat tgcagatgtt gcagcacaga aaaaggctaa agatgtcact aaaattacat
      481 ctgatggaac caatgctaca gtaactttaa aagatggtac agaagttaca gatacaattg
      541 ctaacattac aaccacctat ggtatagaag caaaagctaa tttcaaagga tctgctacaa
      601 ttagcaaaca attagagaac gagaatacta atgcagcggt aacgggtaat tataccggaa
      661 atttgaatga tcttgatccg gaggctgaag ttaaagtgaa actggcaatt ggagcatccg
      721 gaagcactgc actgagttct tctgagaaag ctttgatctt aagcttccag gtaggcgctg
      781 ataccaccgc tgagaacaag attgaagtat ccttagagtc tatgagcgct aagggcattg
      841 gtgtaaacgg actgcaggta aatggcacag attccacgaa tgctgatgcg gctgttaaga
      901 ctatcgcaac tgctatccag aaggtatcca cccagcgttc cgcacttggt gctgttcaga
      961 acagacttga gcacaccatc aacaacctgg ataacgttgt agagaatacc accagcgcag
     1021 agagccagat ccgtgatacc aatatggcta ctgagatggt taagtactcc aacaacaaca
     1081 tcctggctca ggcaggtcag gctatgctgg ctcagtccaa tcaggctaac cagggcgtac
     1141 tgtctctgtt acagtaagct taagctgaga tcaggttgtt tccaaagagg aacgaaccat
     1201 atctgtaaga gttatgtaag aaccaaaggg cagtccatga cgggctgccc tttttgggtt
     1261 cttataagac cttttgatca tccaaagatt tcctttccaa ttccttcgaa aaattatcat
     1321 agtaatccat ggctgcctgt gtagaaatag aaagctcagc atcatactca aaaaagttca
     1381 taccaatgtg ctccttttac ataatttttt gtgcttttga gaattcattt tcaaattcct
     1441 ttgaaaattt ttcgtaatag tcaatggcag attgtgtagg gattgatagt agagtctcta
     1501 acttgtggat tgcatcctct tttgacaagt taaagtcctt acatgaccga acaatttctt
     1561 ttgctctgcc ttcctctacg ccatccttgt aaataactct cttttccgcc tcagcatcat
     1621 actcaaaaaa caccatatca ataacctcct tcttattctc aagcaaaaat tccttcaaaa
     1681 tatccctcct gatacatacg tccacggcct gaacgatggc ctgccggttt ggatacacct
     1741 ttgcctgctc acggacttct gcgacaaact gggcgtactc ctttaaaatc tggcaggcat
     1801 ccatcagctc ggagttgaac ccgagattga tattgattac ctgtacctgt aattcaagct
     1861 gaggagcctc ttccttattc cggtaggcac tggacagctt cagggtgctg tattccggga
     1921 gaggatccgt cccgttataa aacactacaa aatgtggagt atccagcatc tgtaacttgt
     1981 tggaataaag caggtcatcc gtgttcagct tcgaatattc acttgcaaga tacaataaaa
     2041 aacgatacgg catattggga ttcaatgtgg actggtgctc aaaaaaccat agatcaaaat
     2101 ccaccacaaa tgacaggtcg ttctgcaagc ttaggtaaac cgcattttcc aaagttgtaa
     2161 tatgcagtag ctccggatct tcataatgcg agtgattcag cgcattgtac agcgagagca
     2221 gcttctcctt gtcatgaaat attttccgga acaccagatc cttataattc cgctttccgg
     2281 aagccggtat tgccacagcc acattccccg gcgcagcagc tatcgcattc ccatacgaaa
     2341 tttcttccgc aacaaaattt ttccagaatc tcgacacagt attcatcaaa tactcctccg
     2401 agaaatagtt gccggttata ttattttgtt ttaactccat aaatctcctt tctgttgcaa
     2461 aacaaatgta aaaataaaat aaaacaaata ttttccatat aaaataaata ataagggaaa
     2521 ctttgaccac tgacgggtct ataagaattt ttgaaagaag aaaaattact tctaatacca
     2581 ccataccata gggattcctt attggcaagt ctgaaaaatg ttacaaaaag gctacaaaaa
     2641 atccagaaaa atgttacaga acgcttctaa agttttactt tcccggcatt ttggggtaag
     2701 ataagcagta tagattccta aggacacaga aagagagggt tactataatt atgaaatttc
     2761 aggacatgcc ttacgaaaga atagattttg ccaaagtgca ggaagagatg ggagaactga
     2821 tccgggagct ggatgcggcg aagagcgggg aggagcagtt cgaggtgcat cagaagtact
     2881 atgcgctgac ggaccgtgtc atgacgctga tgaccattgc gaatatccgc ttcgacatcg
     2941 atacttccga taaattctac gaggaagagc ataaatacta cgatgagcag ggaccggtat
     3001 tcagcaatct ggtgctggct tatcagaaga aattatatga atccccctac agagcttatc
     3061 tggaggagaa gatcggtccg gtagcgttca agaatatgga gctggcacag aagtccatga
     3121 gcgaggcgtt gatcccgctg atgcaggagg agaattccct gactatggaa tatgataaac
     3181 tgattgccag tgccaagatc gactttgatg gcaaggaact gaacctgtcc ctgttgcggc
     3241 cctatctggt gaattccgac cgcaatgtcc gcagacaggc atgggagaag atgagcgcgt
     3301 ttttcctgga aaatgaggaa aagttggaca gtatctacga caaactggtg aagaaccgta
     3361 ccgcacaggc cagagccatg ggttacgaga attacctgga gctgggatat taccgcatga
     3421 accgcaactg ctacggcaag gacgaggtag aagctttccg caggcagatc aaggagtatt
     3481 tcgtaccctt tgcagagaag ctgcatgacc gcagaagaca acgtctgggt ctggagaagc
     3541 tcagctatat cgacgagcag gtatatttca gggatggcaa cccggcaccc accggaaccc
     3601 cggaagagat catggcagca ggacagaaga tgtaccgtga gctgtccccg gagactgcgg
     3661 aattttttga tttcatgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
     3781 gaagatgtac cgtgagctgt ccccggagac tgcggaattt tttgatttca tgatggagaa
     3841 ccaactgttt gacgtattgg gccgcaagac caagaaagct ggcggttaca tgacttatct
     3901 gcctctgtac catgcaccgt ttatcttcgc taactttaac ggtaccagcg gagatgtgga
     3961 cgtcatcacg catgagtgtg gtcatgcgtt tcaggggtat ctcgctgcac aggatcctat
     4021 cagagagcat gcggatatcg gcatggagac tgcagagatc cattccatgt ccatggaatt
     4081 tttcacggaa aagtggatga acctgttctt cggcgacaga gcgcaggatt acgtggaaat
     4141 gcatctggag gatgcggctg cgtttattcc ttacggctgt atggtggatg agttccagca
     4201 tatcgtctat gagcacccgg agctgacccc ggccgagaga aagcaggcat ggagccgtct
     4261 ggagcgggag tacaagcccc acctggatta cgagggagat cctttcttcg gacagggagg
     4321 cttctggcag aagcagctgc acatttacga ctaccctctg tattatatcg attattgtct
     4381 ggcacagacc tgcgcactac agtacaaggt gaagatggat gccgatttta cagaggcgtg
     4441 gaagagctat ctgaagctgt gcaggctgtc cgccagtgat ttttacacta atatgatcaa
     4501 agaagtcgga ctggacagtc cttttgagcc cggatgtgtg aaaaatatcg tggaaaaact
     4561 tgaaaaatat gtgaaatagc tttctttttc cgcagaaata cggtaaaata aggagtcatt
     4621 gggacttaag acaccaggaa ggagacatgt aagcaatgtc taagaatgaa aaagttacag
     4681 aaaataaaga acaaacagaa cagaaggtca tgaccaaata tgaccgtaag gtacagaagc
     4741 gtaaagagga gaaggaaaag gagaaaaagg aagagcgcat cagcacggcc atcggcatcg
     4801 tagtgcttgt ggctctggta tgtctggtag catctttccc catccgtacc tatctggcaa
     4861 ctcatgagac ctatgtggtg gtcaatggag aagctgtaaa caaggtagag ttcgattacc
     4921 aatacaacct gaccaagaac aattatatca cccagtacgg cagctatctg acatacttcg
     4981 gactggatac cagcaaggat ctgtccaccc agatgtattc cgatactctg acctggcagg
     5041 actattttga gcagaatgct gtagagagcc tgaagcagaa taaggcgctg atggcagaag
     5101 ccaaagcagc cggcttcacc tacgatacca cggatgagta caacactttc aaggagacca
     5161 tcaaaacctc cgctgcatcc gcaggcatct ccgagaagga atatgtacgt tccatctacg
     5221 gaagctatgc aaccatgggc cgtatcgaag aatatgtgaa gaacgacatg gtaatgaacg
     5281 cttattatca gaagctgcag gaggacaatg ctcccagtga tgatgagatc cagagctatt
     5341 atgaggaaaa taaggcaacc tacgattccg tagactannn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnngat accgcttacc agccttccga tgctgagatc gccaaggcaa
     5521 tggaagatgc gaaggtactg gcagatgatg cggagaagac cgtggcaaaa gacggcgaag
     5581 cacacgagaa tgagaagaag tcttccgtga actacctgat ctccgactgg ctgtttgatg
     5641 atgccagaaa ggcaggagac accaccgtca ttactaacga caacagccat tgctactatg
     5701 ccgtggcatt tgagaagaga tatctggatg agactccttc cgcagatgta cgtgtgatta
     5761 ttcccaccga ggataagacc ggtgaagaga tcctggagga gtggaagagc ggtgcagcta
     5821 ccgaggacag cttcgcagaa ttgtgcaaga agtatacaca ggatacttct gcagtagaaa
     5881 acggcggtct gttcgagcag gtgaccaaga ccggtatgac agaggagctg agcaactgga
     5941 tcttcgacaa cagccgtcag gcaggagata ccgtagccat cactgtcagt gacaccacct
     6001 atgtacttta ctacatcgga caggatcagc ccgagtggaa gatcaacatc aagaatacgc
     6061 tggtatccga tacaatgagt cagcatatgc aggatattac cgcagatgta acagtagagg
     6121 atcccaaggg taaactgaat tacctgaagg tgcaggcaga ggaatccgca gccgctgaga
     6181 ccgcgaccgc agagacccag gagatcaccg aggagcagac tgctgctacc gaagagaccg
     6241 caacacaggc acagtaaagc ttgctcagga acagaacata gacaacagac aagtacctgt
     6301 ttcactgcac aagcagaaga gacaggtact gttaggttat gagcaaaaat agtcgcaaaa
     6361 gggcgggaaa catgataatg tgactctgcc agttacgtga gctgaacaga gaataacttt
     6421 gagaaaggta tgaataaaca tgcaggaaaa agttctggta ctggatattg acggaacatt
     6481 gaccaattca aaaaaagaga tcacggaagc taccaaacag ggaatctgga atattttaca
     6541 gcatggtcac aaggtgatcc tggcttcggg tcgtcccacc cccggcatgc ggaaatgtga
     6601 agaggagctg gaactgaaaa aatacggcgg atacctactg tcatttaacg gagcccgtat
     6661 cgtggagtgt aagacaggag aagtggtcta tcagagaaca ctgcccctgt ccattgtgcc
     6721 cggattgtac gaattcgcca aagcgaatgg ctgcggactg atcacttatc agggtcctgg
     6781 cgtcatctct gcgttcgagc cggatgacta tgtgcagctg gaagccagaa tcaacggaat
     6841 tcctataaag gtcgttgaca atttcgtcga atatgtgaat ttcgatatta ataaatgcct
     6901 gatgacagca ccggaagaga tcgcaccgga gctggaaaaa caactgcagg aaaaatacag
     6961 tgatgtggca agcatctacc gttccgaacc ctatttcatc gagatcatgc cgaaagatgt
     7021 gaacaaagcc acctctttgg atcatatgct ggaaggcatg ggactgacca gagagaatgc
     7081 gatctgctgc ggggacggtt acaacgacat ctccatgatc gaatatgcgg gaaccggcgt
     7141 agcaatgggg aatgcccagc cggcggtcaa ggatgcggcg gactatatca ccggcaccaa
     7201 cgatgaagac ggactggtgg aagtcatcga taagttcatc atgaacccgg agcaggcata
     7261 gcctttctta gaaacaaaca atagacacaa agcagtagca agcgaaaact cacagagcaa
     7321 tgagcgtgtg tgatttttgc aaaagaggat atatatgatt cagatacaaa tacctgaaaa
     7381 agcgaaatat atcatagaaa ccatacagaa tgccggattc gaagcctatg tggtaggcgg
     7441 ctgcgtgcgg gattccattc tgggcagatg cccggaggac tgggacatta ccacctcggc
     7501 gagaccggag caggtaaagc agctgtttcg tcgcaccatc gacaccggaa tccagcatgg
     7561 caccgtaacc gtcatgctgg acaaggaagg ctttgaggtt acgacctacc gtgtggatgg
     7621 aaaatacgaa gacagccgac atcccaaaga agtgacgttt actccgaacc tggaggagga
     7681 cctgaaacgc cgggatttta ccatcaatgc catggcctat aatgaaacgg aaggcctgat
     7741 cgacattttc ggcggattaa aagacatcga ggcgaagctg atccgatgcg taggcaaccc
     7801 ggaagaacgt tttggagaag atgcgctgcg gatcatgcgg gcaattcgtt tttcagccca
     7861 gctgggctac gagatccacg aggacacgga ggcagcaatc cggaagctgg cgccgaccct
     7921 gcagaagatc agtgcggagc ggatccaggt ggaactgact aaactgttgg tgtcccctca
     7981 tcccgacacc ctcagagaag cttatgacat gggtgtcaca aaggtgatcc taccggaatt
     8041 cgatgccatg atggagacac cgcagaagca caagcatcat aaatacaatg tgggtgagca
     8101 tacattacat gcacttatgg agatcgggcc ggacaagaat ctgcgttatg ccatgctgtt
     8161 ccatgacatc ggcaagccgg ccacacttac tgtggatgaa gacggaatca ctcatttcta
     8221 cggacacccg gcggtgggag aagagatggc gcggaagatt ttgcggagac tgcgatttga
     8281 taatgatacc gttgcggtgg tcacccgtct ggtgagatac catgattatg gcaatgatgt
     8341 gataccggat ctgcggattg tccgccgggc ggtgaataag atcggggaag atattttccc
     8401 actgttgttc ccggtgaaac gggcagatat tttggcacag agcgattacc tgagaacaga
     8461 aaaactggag aatctcgaac tctggaagaa actgtatcag gaaatgctgg aaaagaagca
     8521 gtgcgtctcc ctgaaaacac tggcggtcac cggcagggat ctgatcgaca ccggtatgaa
     8581 accgggcaga gagctgggag agatgctgca acagctgctg gaactggtat tggaacatcc
     8641 cgagcagaat accagagaac agctgctgga aaaggtaaag gaactgtcgg cagacaaaga
     8701 ttgagcatac tttacaggct ttcctcataa gatagcgtag gaaccggaaa acggttaagg
     8761 acgtatctta ggagggattg taagtgaatg acagagcagt ggcactgctg gagcagtatg
     8821 agatagaagt attgcgtacc cgaaaaggaa ggggagcgat tctgtgcgat acacagcagg
     8881 gctgcctgat cttcaaggaa tatacaggac gcgaggaaca gctgcgtctg caggagcagg
     8941 tcctgaagca cctggaggaa cggggaaccg ttcctgcgga gcgattgctt gccacaaagg
     9001 aaggcgcgtt atcggtgaca gaccgggacg gtgtcagata tattctgaag acctggtggg
     9061 aaggccgaga atgtaatatc cgtgacaggg aagaatgtat ggaagccatg cggcttctgg
     9121 cgagactgca cggtgatctg gaatttacac cggtatttcc ggagacggag gagacttcgg
     9181 caccggtcat agagagatat ctccccagcc gggactatga gaagcataac cgggagctca
     9241 agagagcccg caggtttttg aaacagcgca gccagaagac ctggttcgag atccggttgt
     9301 ctgcagtgat tgatcctttt ttagaggaag caagacaact gtgtgaagaa tggaaagaga
     9361 tcgagctggc tgcctccgat agcggagagg aagcaccgct gtgtttctgt cacggagatt
     9421 accaatatca taatattctg cggcaggacc gtggattttt cctggtaaac ttcgaaaaat
     9481 gtcaggcaga cgggccggtg agagacctgt atctgctgtt gcgaaagctt ttggaaaaaa
     9541 gtgaatggga tgccgaatgg ggcagagtgc ttctggcggc ttatgaaagt gtcagaccgt
     9601 taaagcccta tgagagacag gatctggtct accgcctgtc ctatcctgag aaattgtgga
     9661 agatcgtaaa cttttattat aattccggca aggcctggat cccggagaag aatcaggaga
     9721 aactggaccg actgttggaa caggaagcgg ccagaaagaa attcctgaaa ctgctgcaaa
     9781 gataaaagcc gggcaatgaa cggcagacaa cggaagagac gttgcggcag gatcgcagcg
     9841 tcaggagaca aaggagaagc aaaaacgttg aaaagattcg aagacaaaaa gtattcgaaa
     9901 acgaaacaga cagtggtaat cctttcgctt gtgacagcaa tgtcatttgc tggatgcggg
     9961 cagacagcat cctccgggac ggcggcaaca cctgcgccaa cggcacaggc agagcggata
    10021 gataccggct ttgttgtcac caatccggac agctatgatt ccgcagatac ggcggtactg
    10081 gtggatatca ataatgttga caacactgtc acactgttaa atctggattt aggcaaacgt
    10141 tacacgctgt ccatggacgg aacaacgcgg ttcgcagata aatatggaaa ggcaatttcc
    10201 ctgagtcagc tgcaggaggg ggacatcgtg gatgtgactt ttttgaaaag tcagaagcat
    10261 ctgacctcca tgcagctggc gcagacagca tggcagtatg aagatgtgga gcactacgag
    10321 atgaataccg tacggaatga ggtggccatc ggggaagagg tgttccagct gtctaaggat
    10381 acccagtatt tttcccaggg acacagcatt gaaaaaatgg atctgaatcc ggcggatatt
    10441 ctgacgtttc acggcattga cagcacagtg ctcagcgtaa cggtggaaaa aggccatgga
    10501 tacctcagac tggtcaatga tgagaacttt gtgggcggct ggctggaaat cggccagaca
    10561 cagatcgtac ggatcacaga ggacatgctg gtgactgtgc cggagggaag ctatcaggta
    10621 gatatcagct ataacggagg tggcggcacg aaaaatgtcg taatcaaccg caacgaggaa
    10681 acaacactgg atatcggtga tctggaagtg gcagaggcac agtacggtat ggtgctgttt
    10741 tccctgaatc cttccgatgc agaattgtat atcgatggaa gtcaggtgga tgcttcccag
    10801 ccggtcacgc tggaatacgg tattcatcag atcatagcga aggcggacgg ctataagtcg
    10861 ctcacacagt atctcagggt gggacaggaa tccgcaggag tggatgtcca gctggataag
    10921 gcggactccg acagtgaaga cagtacggaa agcagcagtt cttccagctc ttccgcaacg
    10981 gaatctacct ccaccgcaga tacgactacg acctactacc gcgtgcatat tgatgcgccg
    11041 gaaaacgtgg aggtatatct ggacggcaac tatgtgggaa tttccccctg cagttttaag
    11101 aagacctcag gaagtcatgt gatcaccctg cgaaaatccg gttatgaaac cagaagttat
    11161 acggtacagg tggatgaaga agaaaaggat gtatcctact catttgtaga cctgacggcc
    11221 agctcttcat cgacagaatg atcctttccg ggaggggaag aaatgccgta aatatgcgga
    11281 aaaaccttga caaaagggat aaaaacagat atatatatgt ataggcgttt caaaagaact
    11341 attttcgagc gtaaaattaa taacgaaaac tcattacagg aggagttcaa tatgaacaaa
    11401 acagaattag ttgcagctat ggctgatcag gcaggtcttt ccaagaagga cgcagagaag
    11461 gcattaaagg cattcaccga tgttgttgct gaagagttaa agaaggatgg taaggttcag
    11521 ttagttggct tcggtacttt cgaagtatcc agcagagcag ctagagaggg tagaaatcct
    11581 cagagcggcg cacctatgaa gatcgctgct tccaaggctc ctaagttcaa ggctggtaag
    11641 gcattaaagg atatgctgaa cgcataatag aagctgaatt ttcagagact cgataggaca
    11701 acctttcggg tctcttttgt ttttcagaaa cggatgatcg tgaggttaaa agcatgagat
    11761 tagacaaata tttgaaggta tcccgactga tcaagcgccg tactgtggct aatgaagcct
    11821 gcgacagcgg cagagtgctg atcaatgata agccggcaaa ggcatccgcc aatgtcaaag
    11881 agggagatat catttccatc tctttcggca acaaggaagt aaaagtcgag gtcctggatg
    11941 tacaggagac cgtgaaaaaa gaagaagcca aagaactttt ccgttatata taatatcagg
    12001 ttctctatgt ataaatttcc acattttccc atacaataca ttgtaagtcc ggttccggaa
    12061 ggagcggagg atgggaggct atggagagaa tggaagattt aaacgcgaca ggacgtatgc
    12121 ataaagtgac catgaccaac agacggacat gtaccgtgaa tggggtaaat gacgtccttt
    12181 cctttgatct gcgggagatc ctgctggaga cggaacaggg gatgctgatg atccgtggca
    12241 acgagctcca tgtgaaccgc ctgacattag acaaggggga agtggatatt gacggccgga
    12301 tcgacagcct tgtatattcg gataccgcag catccggaga aaaacaggaa tccttcctgg
    12361 gcaggctgtt ccgctgatgg atgaaaatgt ttttctgctg cattcctttc tgctgggtgt
    12421 ggggatcact tttctgtatg atattctgcg gatcctgcga agagtgtttg ctcacagtac
    12481 cagcatggta tctctggaag atctggcatt ctggatctac tgtgcgggaa aggtctttct
    12541 gctgatgttt catgaaagta acgggaacct gcgatggttt gccgtgctgg gggcattggc
    12601 gggaatgtac ctgtatcaca ggacaatcag tccctttttt gtaaagtatg tggcactggg
    12661 cctgaatact cttctgcacc caatccggaa agccttgcgg ataacgggaa attggctgaa
    12721 aaagcagttg actatactta ggaaaatgct taaaatattc attaaaagga gatgcgcgta
    12781 tggcaagaag aaaagcagcc taccgcaaga gacaccagaa ccggttcagt atgttcctgg
    12841 tgaccctggt cgtgttgatg atcatggtgg tcgttgcggc gcggagcgtg gaattacaac
    12901 agaagattgc gggctatgat acccagatcg caagtcttgg cgcgcagatc gaagcagagc
    12961 agaccagagc ggaacagatc gaggaattcg gtaagtacac tcagacgaaa gcttatgtgg
    13021 aagaggtggc gaaggataag ctgggactgg tatacgaagg tgagatcctt tttcaggaag
    13081 acaattaagc agggaaactg tttctcaaca gtgaacctgc tttttttgtc gcaaaaaatg
    13141 aagtctctcc ctccctcgat ttgacaaaag gaagaggaaa actgccctat aatcgtgaaa
    13201 acagaaaggg gaggatgcca tgatcatagg gaaaaaggag caggaacggc tggagagaga
    13261 acagtgtgca cagatctcac tgctgtgcag acagaaactg atgggatatg cggagagttt
    13321 tgaggaactg gggaagagct ttcggcagga gatacagatt acaggagagg acaggcagaa
    13381 tattctggaa aaatgtatgc tgtggcagag cagacagatc atgggagacc atctgcagga
    13441 agtggcccgg atcatggaac gggtagcagg agaggaactg gggtattgtc cgctggaaga
    13501 gaaaaagagg aaaagcctgg tacaggcgct caagagtgaa gggatcatcg ccagagacct
    13561 ctgctatgtg gagaaggacg gcagcagctc cgggacagaa gcagggatca gtatgacact
    13621 gtccaccgaa aaaaggcact ataaggcggc acaggtggcg gacatgctga cggtattatt
    13681 gggaaaaaag ctgcaggtct cttcaggaag cccttatctc gtggagcagg agccgcattg
    13741 ttttctgttc acagaggaac cgaaatacat agcactgacg ggggtgagcc gggcggtaaa
    13801 agaaggggag aaagtctcgg gggatcagta tgccaccatg gaatcggaaa aagggaagct
    13861 gatcctgctg ttgtctgatg ggacaggctc cggggaggat gccagccggg gcagcggcag
    13921 agtcatggat ctcatggaaa aaatgctgga ggcgggcttc gggaccgagg catctgtgaa
    13981 tctcctgaat tccgcgttgt atgcgcaaaa tgaagaggac gatcatccta cgatagacct
    14041 ttgcagcctg gatctgcata ccggcgaatg tgagatctgc aaggtgggcg gtgttgccac
    14101 gttctggaag acctccggaa gggttctctg tgtcggcggc gaaagcctgc cgttaggtat
    14161 ttttcaaaaa gtgcagatag aacgacagat gtgtcagatc cggccgggag atctgctggt
    14221 catgatgacg gacggggttc tggatatgat ggaagacggg gaagagcgca tggcacaggt
    14281 gctggaaggg ttgcaggaac agaacccgca ggagatcgca gagaaaatat tgtcctatgc
    14341 catctgtgcc tcagagggac ggattcggga cgatatgacg gtatttgtgc tctgcctgtg
    14401 ggaaaacacg tgatgccgcc ttgatttttc cccataatta ggatagagta aaaacatgaa
    14461 tacatggatg gatacgcgag aaaaagtatt tgcatatatc agagagcatc acatgctgca
    14521 ggccggcgac cgtgtggtag ccggtgtttc tggaggggca gactcggttt gcctcttatt
    14581 tttgttgctg gaatggcaga aggaagtacc aacagacatt gccgtagtgc atgtggatca
    14641 cggcattcgc gcggaggccg gagaagatgc ccggtatgtg gagcagctct gcgaggagag
    14701 aggaatcctt tttttcctga ccagggcaga ggtaaggaac agagcccgca tggagaaaat
    14761 ctctgaggaa gaagccgggc gcagaacgag atatgaggcg tttgaaaagg cggcaaagga
    14821 gtggggagcc acgaagatcg cagtggccca caacagcaat gaccgcagcg agacccagct
    14881 gttccatttg ttccggggca gtggaatccg gggactggcc agcattttgc ctgtgagaga
    14941 tcggattata cgaccgctcc tatgtctgga acggtgggag atcgagaagt ttttacaaca
    15001 gcgaggaatt gtctattgca aggatgccac caatgaggag gatgactaca ccagaaatcg
    15061 tattcggcat catattttgc cttatgcgga gcagaatatt gtaaagggct gtgtagcgca
    15121 tatgaatcag acggcggagc tgctggcgga gacggaggac tatctggaac tgcagacaaa
    15181 agaagcagct ggccgatgta tccgggcaac ggacggtgac ggagattccg tagagacggg
    15241 ggacagccgg aacgcggtga tctgtattgc tgtggagtct tttctgtcgt tgcatcctgt
    15301 gatccgtaag cgcctcttgt atgaagaagt gaagcaattg tctcccgggc agaaagacat
    15361 cacttaccag catattcagg agctgctgac cttgtttacc agggaaggta acaggcagat
    15421 ctgtcttccg ttcggcatcc ggggtagacg gcaatatgag gaagtcctgc tgacagtaga
    15481 gaggacagac agggagcttt tggagaagac ggaagtggct gagaaatatc aaccaactct
    15541 gcagctttct cccggacaga cagggacagt ggaatgtgaa gtgggaacgg tggaatactg
    15601 tgtgtgtgac ctggccgctg cccatgaaaa aaaccaagaa gttcccacaa atcagtatac
    15661 gaaatggttt gattatgata aaataaaaaa atgtccggtg atacggacaa gaaagccggg
    15721 tgactatctg accattgctg acggaagagg cgggatgatc cataaatcgc tgaaaagtta
    15781 tctggtcgat caaaagatcc cgcaggcgga acgcgacaga ctgccgatcc tggcagaggg
    15841 agagcatgta ctgtgggtcg tgggataccg tatcagcgaa tattataaag taacggagaa
    15901 tacaaaacgt attttaaagg tacaattaat aagaaaggag ttcgcttaaa agcgaaaaga
    15961 caaaagaaaa tggcagagca tatcagagta ctgttgacag aagaggaagt agataagaga
    16021 atccaggaga tcggtgagca gatcagcaag gattatgcgg gtaagcaggt acaccttgta
    16081 tgtgtactga agggtggcag ctttttcatg tgtgaactgg caaagcgtat cactgttccc
    16141 gtatctctgg actttatgtc cgtatccagc tatggcagcg ataccaagtc cagcggtgta
    16201 gtcaagatcg tgaaggatct ggacgaatct ctgaaagata aggaagttat tgtagtagag
    16261 gatatcgtgg acagcggaag aaccctgagc tatctgctgg agatgctgaa ggacagaggt
    16321 ccggcttctt tacgtctgtg caccctgttg gacaaacccg agagaagagt gatcgatgtc
    16381 catgtggatt ataccggatt taatattccc gatgagtttg tagtgggcta tggtctggat
    16441 tatgatcaga gatatcgtaa tctgccttac atcggtgtgg tagaattcga ttaagcgata
    16501 caaagatgta ggaggcccgt gaagttgaaa caaaacggca aaacaatcaa ttcatatttt
    16561 atatttatca tacttctgct tctggtgatc ttcggcctca acctgttgaa caaccgtaca
    16621 gatgaataca ccaaggcgga gttcattgcg gatctggatg ccggcaatgt atcggaagtc
    16681 gtggtgcagc ccaacggaga agcacctacc ggatatctgg agatccagat gaagaacggt
    16741 gcatctcata agctgtacgc aacggacatt acagaactgg agaccctggt acgtgagtat
    16801 gggtttgatc cggtggtcaa tgacattgaa agggagaact ggttcctgac ctatatgctg
    16861 ccgatgctgg tcgtgctggc ggtgggaatt ttcctgttca tgatgatgaa tgcccaacag
    16921 gcaggcggcg gtaacggcaa gatgatgaac ttcggcaaaa gccgcgccaa gatgacactg
    16981 ggtgacaaga gtgtcacatt cgctcaggta gccggtctga aagaggaaaa ggaagaactg
    17041 gaggagatcg ttgatttcct gaaggagccg ggaaaataca ccggtgtcgg agccagaatc
    17101 cccaagggtg tcctgttgga gggccctccc ggaaccggta agaccctgtt ggccaaggca
    17161 gtggccggag aagccggagt accgttcttc agcatttccg gatccgattt cgtggaaatg
    17221 ttcgttggtg tgggcgcttc ccgtgtgcgt gacctgtttg aagaagcaaa gcataatgca
    17281 ccttgtatcg tatttatcga tgagatcgat gccgtggcaa gacggcgtgg taccggtatg
    17341 ggcggaggac acgatgagag agagcagacc ctgaaccagt tactggtaga aatggacggt
    17401 ttcggcgtca atgagggcat tatcgttatg gcggccacca accgtgtgga tattctggat
    17461 cctgcaatcc tgcgtcccgg tcgtttcgac cgtaaggtag cggtgggaac cccggatatc
    17521 ggtggcagag aagagatttt gaaagtacat gcgaagaaca aacctctggg ggatgatgtg
    17581 aatctgcagc agatcgctca gactactgca ggcttcaccg gagcggatct ggagaacctg
    17641 ctgaacgaag cggccattat cgcggcgaag gagaaccgct cctttatcgc acagaaggat
    17701 atcaagagag cctttatcaa agtgggaatc ggtgcggaga agaagagccg tatcatttcc
    17761 gacaaggaga agaagatcac tgcataccac gaagcaggcc atgcgatcct gttccatgta
    17821 cttccggatg tgggaccggt ctataccgta tccattattc ctaccggtgt gggagccgcc
    17881 ggttacacca tgcctctgcc ggaaaaggat gagatgttcg ccaccaagag ccgtatgctc
    17941 caggatatca tggtatcttt gggcggacgt atcgcagagg agatcatctt cggagatatt
    18001 accaccggag catccagtga tattaagaaa gccaccaaga cagcccgccg tatggtaacc
    18061 cgttacggaa tgtctgataa tatcggtgtc atctgctatg acgatgacga tgatgaagtc
    18121 ttcatcggta aggatctggc acatgccaag gcgcacagtg aggagatttc cggtgagatc
    18181 gataaggaag tcaagcacat catagatgac tgctatacca aagccaagga tattatctta
    18241 cagcatgagg atgtgctgca tagctgtgca cagctgctgt tggaaaagga gaagatcacc
    18301 agagaagaat tcgagggtct tttcgtatag gaaaaggttt tcgcgggagt ttcgtggaat
    18361 tggtagtatt gtacaaaccg attttgtgaa aaatatacaa aaatgaatat tcacttttgt
    18421 gatatttgtg aattttgaga atagtcaaaa ataggagtgc atgctattat aatactcgta
    18481 acccccccaa tacattatat agttttttgc tataccccat aagaaagaaa taccttctct
    18541 aaaaagggca gtcctcccaa gactgtcctt tttactgtct aaaaatcgct gaataaagag
    18601 gcgggcagtt gtcagatgta aggaaaaagg gtataataaa tagagctttt gagcttggga
    18661 catgtcatac tgccggagcg cacaagacaa caaagcactt tggcagaagc agtgtcataa
    18721 tatagatagt aagacattag tctcagaaag ggtagatacg atggatctgg agcgattacg
    18781 tcgaacagaa tataatcagc aatacattgc ggccatgaga cctgtattta acaggagagc
    18841 actttttaca gataccagtg cggaatatgt gattccggaa gagccggcct gtttttccga
    18901 agtgacgatc cgcttccgga ccgccagaaa taatgtggac agagtgttcc tggtatgcgg
    18961 cggacagaag catcttatgg tacgtgtgga gagcaaaaat gattttgact attatgccta
    19021 cgtaatgcgt ctggatgatc agaaggtatc ttattatttt gaggtgcaga ccggcagaat
    19081 taccggcatc tttgatatgc ggggcctggt gcaggaagtt aatgaatatt atgatttcat
    19141 tatcattccc ggtttccaca cccccgactg ggccaaggga gcggttatgt accagatcta
    19201 tacggatcgt ttctgcaatg gagattcttc caatgatgtg ttgaccaatg aatattgcta
    19261 catcggagaa ccggtacacc gcgtagagga ctggggacgt tatcccgcac agatggatgt
    19321 ccgtgagttc tacggcggag acctgcaggg tgtgctggat aagatggact acctgcagga
    19381 tctgggagtt gaggtgattt attttaatcc tctgtttgtc tccccttcca accacaaata
    19441 cgatattcag gattatgatt atattgatcc tcatattgga aagattgtca gtgatgaagg
    19501 ggatctgctt cccgacggac agcgggagaa ccgtttcgct tcccgttaca ttgaccgtgt
    19561 gactaacaag gcgaatctgg aagccagcaa cgagctgttt gctcaggtgg tggcagaggc
    19621 acaccgcaga ggtatgagag tcattctgga tggcgtgttc aatcactgtg gttcctttaa
    19681 taaatggatg gacagagagc gcatctatga gaatgcggaa ggatatgaca agggtgccta
    19741 tgtcagtgct gacagccctt atcggaatta ctttgatttc cacaatcagg cagcatggcc
    19801 ctataacaat tcctatgacg gatggtgggg acatgacaca ctgccgaagc tgaactacga
    19861 gggatcccag gaactgatgg actatgtcct tcatgtagcg aaaaaatggg tatctccgcc
    19921 ctacaatgtg gatggatgga gactggatgt ggcagctgat ctgggtcaca gtcaggaatt
    19981 caaccatcat ttctggcagg aattccggaa agcggtaaaa gaggccaatc cggaagccat
    20041 tattctggca gagcattacg gcaacaccag agactggtta cagggaaatg aatgggatac
    20101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cagatgacat ggacgggtgc
    20221 tcctacaatc tattacggag atgaggccgg tgtctgtggt ttcacagatc ctgacaaccg
    20281 cagaacatat ccttggggac atgaggatca ggtgctgctg gcatttcata aggacattat
    20341 ccgtctgcgc cgggagaacg aggagctgcg gatcggctcc ttgaaaatgc tggaaaatga
    20401 ctacaacttt atttcttacg gacgttttaa ccgcaagggg caatgtgcga tcctgatcaa
    20461 caataacaat catcccatca ccaaagagat tcatgtgtgg gagatcggaa cccctaagac
    20521 aggtaagatg aaagtgatcc tgcagtccag ggatgacggg tattgcatgg aaggtgcgga
    20581 atatgagatc agggcaggca aggttctgat cgagatgcca cagacctcgg caacgatcct
    20641 gaaatacgta gaagaataaa tatatgtaac tattcagaac accattcgca aaaccgcctc
    20701 tgcgaggctt cctttttgcg aatggtggtt acttcgccct ataaatagta tcaaatgctc
    20761 ttacgaatgc aagcattcga cgctcattcg atactattta catggctctg aatagttaca
    20821 aatacaaaag acagagagga tattcgatgt tcaccatcat tattaccctg ctgttgccgc
    20881 tggtatccat tggaatctgg cggcaatcta ttttaaaaaa taacaaaaaa agcggacagt
    20941 ctgtcacgat cgggaaggga gaatatgtcc ttcgttatct tacctgcctg ctctgtatgc
    21001 tggtcattcc ctggatcctg ttgtcgctga ccggcaatga cggcaatacg atcttgagaa
    21061 agctgttgga atccagagaa tatgcagtga aagttctatg tctggagatc agcatgatgc
    21121 tggtctatgc cattgcagaa ttgttcgtgg aagaagcgaa agctggaaag catgagaaga
    21181 tccggagcgt cctgagtaag attaccgata gcaaggcgtg gagcgtattt cgcaaataca
    21241 taggaccggt ggcagtactg gcattgacag tgctggtggt gtgtctgaat ttcagcatga
    21301 tgtccgaccg cgtactgtgg ggggatgagg cgttttctgc gaatacggcc cacaaggatg
    21361 tggatggcat tttgcaggta ttgtattact gggacaatca tccgccactg tattattact
    21421 ggctgaaatt attcgggacc ctgttcggat ataaggtacc ggtattccac ctggcctctc
    21481 tggtgccctt cgtgatcggg atcgtactgg cactgaccgt tgtgcggaaa catttcggaa
    21541 tgcttgcggc aacatttttt gtgatgatct ccggtctggg acaggcgtgt ctggagtaca
    21601 atctggaagt ccgcatgtat gcgctggctt tcctgtgtgt aatgggttgc ttctattgtt
    21661 cctacagagt cattgctgac ggcagcagga agacctgggc aggcatggtg ctgtgggcac
    21721 tggcagcagc gtattctcac tattatgcac tggtggctgt aggcattatg atgtttttca
    21781 ccggagtggc cgtatggatc aaataccgtg ggaaaacatg gataaagggt gttctggcca
    21841 tcgtagcatt tttcataggt tatgcaccct ggctatattt cttctatgcc ggcttgaaaa
    21901 atgtcagcag aggctggtgg atgacagaga tcctgggact ggaccagagt ctggagatcg
    21961 ttatgggcgg cagagggatg aatggtatcg tattcccgtt ggtgattctg tttctggtgg
    22021 tgaccctggc agtggattct tctgtattct ctgtggaaaa agacggagta catatgcaga
    22081 agccttctgt gagaaactgg tctgacaaga catatgccat ggcagtggga gcctgcacaa
    22141 tccttggaac tttggcattt gcttatttgt taagtgttgt tatggcaccg atgctggcac
    22201 agagatatct gtatccgtta agcgcggtgg caatcgtcat gctggcggtg ggcagcagcc
    22261 gtgtgctgga actggctgca gagctggaga agaagtcctg gaagggcctg gaggctgtaa
    22321 gcagaattgt gctggcggta ttgctggtag tattattcgg cctgggaatt cagaactatc
    22381 gggaatgcta tgacagctat gaacagcaga aggtggagac cgaaaagaca ctggatctga
    22441 tcggtacccc cgatgaggat gtaaatatgg tcacgaatgg tgtaaaacat ctgggttgga
    22501 ccgtgctgta ttattactat ccggataatg aaatcgtgaa cggagattac aaccaggcgg
    22561 attccgacag gttctggtat tttacaccga ccgaacttgg cgcagatgcc atcgccggat
    22621 tgcagcagga tggatatcag gtaaccgatt acggactgat gcaactgtcg cagtatcctt
    22681 tctatttata ttatattgaa gcagttcagc ctgcgccatt cgcaaagttg cgttagcacg
    22741 ctttttcgtc acttcgtgac tacttttgct catagattgg cgctctgctc ataggatgac
    22801 agaattgacg ctttgtgtgc gagcacccaa tcgtcgcttc tgtcatccta tggctgaact
    22861 tttacactaa aataataaaa acatcttgca atttgtgtga acatatgcta caatttcaca
    22921 ttgttaaggc actcttgcct gacatggatg gattcccgag tggccaaagg ggacagactg
    22981 taaatctgct gcaatttgct tcggtggttc gaatccacct ccatccattc gcgttgatga
    23041 gctcaatgga acagtgctta gcgcgtaagt ccatgcagta gagtataacg caatgcgtcc
    23101 gctggtgtgg cggaataggc agacgcaagg gacttaaaat cccacacgta tattccacac
    23161 aatatcgtgc aaacccctga ttttactggg tttccagaaa atcaaaaaat tttaatttgt
    23221 tcatccatcg gaaatatccc tccaaatatc agggaaaacc cctccgattg atgtatatac
    23281 acaccatgct ggtgtggcgg aataggcaga cgcaagggac ttaaaatccc tcgggggcaa
    23341 ctccgtgccg gttcaagtcc ggccaccagc agtatggcat gaggaagaat cccggttagt
    23401 ttgctgctgc tcggggttct ttttctttgt aatttggtat gattccggct atcgcatttg
    23461 cggatataac cttgttctta tagttcagat gagcatagat attcatggtg gtggaaatat
    23521 tactgtgtcc cagccattcc tgaattgctt tcatgtctat accgctgtca tataaaagcg
    23581 tagcacagct atggcgtaaa tcatggaatc ttatatgctt cagctcattc ttgtcacaga
    23641 taagcctaaa gtgctgtgta acaaaattcg gcttaattaa attccccata ggatctacat
    23701 tgatatattc tctgaactcc ttgttatagg aatcaccaaa cagttttgca ttaagctgct
    23761 gattacgcag cagtttaaca agtagttctt ccagctgggg caccagaggt aaagacctgt
    23821 tactggattt ggtttttggt ttgtccttct ttatcatttt cagcttgcca tcgatacagg
    23881 actctacgac gacgtgcttt acgctgattg ttttacggac aaaatcaatg ttcttccatt
    23941 tcaaaccaat aatctcactt ctacgcatcc cataataact tgcaaaaatt acaatctgtt
    24001 cgagaggatc tccgcgaaaa acccggaaca tctgatccaa ttcttcagca ttgtaaatac
    24061 tgccgacaaa aacttcttta ataggctttt taacacgatc agcgggattg cagtttatgg
    24121 ttccgtctat atacaattcc tgcaatgcct ttcgtatatt tgcgtgtctg tggattacag
    24181 tatttgcaga tacttttctg gtatccatta aataatcgta ataatccttt atcagctgtg
    24241 ggtttttctc aagatctgac agagtaaaac ctttttccaa aaaatatgga taaacggaac
    24301 tttcaatgcc acattgatag ccagcgaagg tatcttcatc taccgaatgt ctcatggatt
    24361 taagccagaa cagcatataa tcacaaaata aaatctgatc gactggttgt agcatgagat
    24421 tagggttaac gggtggaatt acccgttttt tcttatttcc ctgtttgttc gtattcttga
    24481 atgaagtaag attcatcaca tcgggtttat ccatagtggt ttcagaaaaa ccattcagat
    24541 tacgattatc tgactgatgt gcaacaacat cagtatctaa actacttaat tcttccggat
    24601 tcacttccct tccttgctca gcagccattt caggcggtgt aaactcttga cgtgcttgtt
    24661 ggagcattct ttcggcattt cttgcatttc ctctgatttt caggccggta gagatttgtt
    24721 ttgtctttct ctttccattt gcatccttat aactcagaat aatctggtaa ataccgtttt
    24781 gctccctcag atgacctgct accatatata tttcctcctt ttggtagtct cagctcatct
    24841 gccatgacaa gagcattata gcatacctaa aatgaaatta gaaaggtatc tgtaaatatt
    24901 ccaaaacata aatttttggt attttataga tacgtccgat acaaacagat ttaatctgct
    24961 tttctttcac aagcttatag gctgttttgg tgctgatgcc gagcatggaa gcaagctgtt
    25021 ctacggttac tacatcaggg tagttttcaa aaatagattt atttgtattt ttcatagtga
    25081 atcctcataa atgataggtt atacacatat ctcccgtcac cgcttcctgc gcccttttag
    25141 gccgcgtagt atggctgtac tgtgtgcatt tagtctatcc gcagtgatag gacggatagc
    25201 acccgtatgc atgacggtat atgctatgaa cttgtcatgg agcaatgacc cataatacag
    25261 atcacttgta gagtaaatac agcagatgat gaaaaatccc tgaaaaattt tttgaatttt
    25321 gtcaatgggc taataacata caaacaatgt tatcagaaga aagttggaat gctttgatta
    25381 ttatagcgag tgttaaaagc cggttgacag agtaatcaca gatatgctat tctcagcttg
    25441 taattgattt ttgtgcatcc caatcgggat agaagcacac gttgtttaag tttgtacgta
    25501 agtgtcaaaa attgccataa gtaaaagtgg catatttttg gcactttttt tgtttccgga
    25561 aaatgggaat gaaaaataag tttatatgtg ggcaatacca cacgcagttt aggaatggat
    25621 tcattgtgca gccattcttt tttgttgcca gaaattgccc atacaagggc ttaatccgca
    25681 aagcggctgg ataaataacc tcgaatgagg agaagaaagg aaggaaaaca aatgaatgga
    25741 gcaagagtgt ttgaagacgc ttacagtggt gtcttcaggg aacaggagga tttcttttca
    25801 tgcctgcatg aaattatggg aaaatcctct tggatccgac aaaaggtcag aaacctgcgg
    25861 ttagttccca ttattgcagg gagtaccttt gagaaagaac tacgcagtca gtatacggaa
    25921 gatggcatgg atgatggcat cataacggat acgattgcaa atacagcgtt aatgctcaaa
    25981 taccggggaa aaatgttacc ggtgagggac tgcgccatca agagcattct ggatcgtgca
    26041 ggtatccagg gaagcggcct tcgcagggtg gagagaaacg tctatgccag aatcttaaat
    26101 gacgtactga aagtcacaaa gggagaggca ttactccgca tcagcgaagg aaaggtatcc
    26161 gcagtattga gcggagactg caatgattac gctatcctta acatggagga gatattcctt
    26221 cacaccgtgc aatatttgca ggaggaattt gaaggctgca ggtatatggc aggcttctat
    26281 gagcatgaca tggtatctgc catgtggagc attgatgagt caggtctgtt atcggcttac
    26341 agcagggaac tttcccttct tgggaagacg acggaagaca tgatccccat ggtgcgggtt
    26401 actacatcag ataccggaac ctgtggagcc aatatctacc ctatgctgtt aaacgtaaag
    26461 gatggaaaga gtgtcaacct ggggtctcca ttaaagcttg ttcataagaa cggcagcaca
    26521 atggcagact atgatgcaca gcttaagaaa ctctatgcaa agtatcagct tgcaatcgga
    26581 aagcttacag agcttttaga agtgcggatc gccaatccgg tgaactgcat gagcgaagta
    26641 atggcaaagc ttgcaattcc taagaaatat caggcagaag cactggactt attcaaggca
    26701 cagtacgatg tctggtcctg tacggcacat gatatctatt tcgctatttc agagatcctt
    26761 ttcatgctgg agtgcgaagg agcttcgggc agccgtatcg caaagatgga agagcagatc
    26821 gcaagggcac tgtcaatgaa ctgggcagag tatgatttcc cggaaatgta aataaaaaca
    26881 aaggagaatg gacaaatgga agaattaaga aagaaactga tggaagaatt aaagaataaa
    26941 ctgggagaag gatatgaggt atccccggta gatgtaaata agaataatgg aacgatatta
    27001 catggaattt ctataatgtt aaaaggcaac ataggaggtc ctacggtata tgtagataac
    27061 ttctatcaga gatttcaggg gtccaatgaa gagattgagg agatgataga ttacattctg
    27121 gaggatatca gagcctgcta tgaaatagac gatgaggagc tacaaagaaa tgtctatgaa
    27181 tatgacaggg cgaaggaaca tctgagagtt gcactatgta attatgaggc aaacaaggaa
    27241 accttatcaa gcagacctca taagcggatc cttgacctgg cggtcattgc gtgtgtcaga
    27301 aatgacgaag ctcccgtggg aagaacgggc tttatctatg tcactgacag attattggaa
    27361 atctggggag tttccaaaga ggaaatgttt actactgcct tgaaaaacac gatgaaagag
    27421 gcattctgtt gcaaactgag tgatctgctg gcacaacagg atgactttga gcttcctccg
    27481 ggagtggaga ttcccttgta tatcctgaca acaaactcca aatatctggg tgcaagctca
    27541 cttttaaatg gtccggcact gaatcagata gcggaggagt tacacagtaa tctgtttatc
    27601 tatccatcca gcatccatga agtattggta ttaccggatg aaggtacaag aaagcagaga
    27661 gcaatgcaga aaaaaatggt ggaagaggta aatgaaacaa atgtttctcc ggaggaaaga
    27721 ctttccaaca gcgtttatta ctttgaccgt gagaaacatg aagtcaccat agattacctg
    27781 ggaaaaccgc tcagcgaact gaacagatag aaacagaatg aaatatagaa cagtggcaga
    27841 aaagagaagg catccccttc tctttttctg tcgtatggaa ggagaaagaa tatgagtctt
    27901 aacttaaatt acaagccgga agcagttgtg gatatttccg gattatcaag agaggaatgg
    27961 ttagattacc gtaagaaagg aatcggtggc agtgatgctg cagcaatcat gggagtttcc
    28021 ccgtttgcaa ccaaaagaga tctcttttac gacaaaacag gtatccgtcc ggcttttcag
    28081 gagaatgagg ataactgggt agcaaaggaa gtaggacaca gactggaaga tctggtagca
    28141 gaaatctttt cgcaaaagac aggacttaag atttttccag tgcgcacgat gttcagacat
    28201 ccgctgtatc gctttatgct tgcagacgtg gactttttca tcgaatttgc agatggtacg
    28261 tatggaatcc tggagtgtaa gacaaccaac tacaactgtc aggaaaaatg ggcaaataat
    28321 acaatcccgg tcaactatga gtaccaggta cggcactata tgtccgtcat gaacattgat
    28381 aaggcctata ttgcctgtct ttacgggaac aatgcagagg agttctttat acgtcccctg
    28441 gagagagacc tggagacgga agaggatctt attgcggaag agaagtactt ctgggaagaa
    28501 aaggtgttga agaaggtgga gccggattac gtagagaaag cagatcttgt gatcgcaagc
    28561 atcaaaaagc attatggtcc agcagatccg caggccgcag gtgtcattct ttccacaaag
    28621 aatctatcag ggatcaagac gtatctgagc ctgaagcagg aaaaagcgaa gttggaaggc
    28681 gaggtaaagc gtttggagga gagaatgaag cagtcctatc ttggtgttct ggaagaatta
    28741 ggagcaagct gcagcggcac gatcatggat ggcaccacaa agtatgaggt gtcctataat
    28801 ccgacctacc gtacctccat tgacaaaaaa ggactggaga aactgaaaat ccagcacccg
    28861 gatgtctatg acgattacgt gcagacgacg gagaaccgga gatttgtcgt aaaggaaaga
    28921 aaggtggcat agcggtatgg agaacagaaa ggttgtattt atctgtgcac cgtatgatgc
    28981 taaggattcc atcgctcacg caaaggaaat cgcacttgta tcgcattttc gggctcttat
    29041 agaggatatg taccactata agacttatgc tcccggaagc taccttaccg ggccttcaaa
    29101 ggaggcaaag gatttccggg tagagatcga agatgtgatc cttaaaaact gccatgcgat
    29161 gtttgtatgt ggcagggaag tgacaggtgg aatgcaggta cagattacaa aggcaaaggt
    29221 attaaatatc ccaatttacc gggtagtgga aggcaaaaag ggagtgatta ttaccatgta
    29281 tgtagaacag gagaaaaaat atgtgtaagg aaagaaagat accagaaata cttatatgcg
    29341 caccttgtga tgcggtcaca gaagaagatg cagaaaagga atatgagctc cgcaagcact
    29401 acaaaagagt gttccgaaga ggagagccta agtgctgcac ttttaaatac attgcttttg
    29461 cgattaataa cgcaaaccta tcagatgcgt tcttcaggaa gagcctacaa aagtcaatgg
    29521 ctaagtcctg tgatatcgtc atagtctgcg gagatgtgat aacaccaaat atgaaagagc
    29581 agattgaaga agcaaaacgg tatcataagg aaatttatcg aatgatagaa aaagaggaag
    29641 gtgcttgtat cttctgttat caagacgagg aggaagcaga tgaaaagtaa gtttgtaaaa
    29701 acggtctatg agaagaatgg attttgcatc tatgtatatg ttacggaagc agaatcggtg
    29761 ccacaggcag ccagaagtaa ttactataaa ggccctggat atgaatttac cgctatcgga
    29821 aataatcttc cggcaaataa gctgtcggaa gtagatctgc atggtgactg ggagaaaaac
    29881 agccacggag tgcagttcaa agtagattat tttgaagaga tccgcccgca gagtgtggaa
    29941 ggaatcgaaa gctacttagc atccggtatg gtaaaaggga tcggtcccaa gacggcaaaa
    30001 ctgattgtcg aaaagtatgg caccagaacg tttgaaattc tggatgatta cccggagtcc
    30061 ctactggaga tcaagggaat caccaggaaa aaactggata aaatactttc ggcatatcag
    30121 gacagtcatg cggtcaggga tctggcagcg tatcttactc cgtttaaggt aacacccaag
    30181 aaaatccgta agatctatga gcatttcggg aatgcggcac tggatgtcgt aaagaaccag
    30241 ccgtttgccc tgtgtgagat cagtggattt gggttcctta ccgtggatga gatcgccaga
    30301 gcgaatcact gcagacccaa tgagccatta aggatcgaag gctgtatcgg ctactgcatg
    30361 gaactggaaa tgcagaacgg taatctctat gccgataagc agaaattcca aaagcaggtg
    30421 catgagcagc taaacagagg ttatgcagaa gaagtagtta ccgaggtaga ggtgtataag
    30481 gtgatgtacc agatggtgca tgacaaagcc ctttactatg aggacggtgc cctgtatagt
    30541 gccaaactct atcattttga gacacaagca gcgaaggatc tggctacact cctggtgcag
    30601 acgtatatgc cgcctgcagg actggaagat ctgatcacgc aggcgcagaa ggatttaaag
    30661 ctgttattat ccgacaagca ggtggaagca gtaaagaagg cattcacaaa tctggtgagt
    30721 attatcaccg gcggtcccgg caccggtaag actacggtgc agagggtact gttatacatc
    30781 agcgaaaagc tgggagaaga aaagatctta ttaactgctc ccacaggcag agccagcaga
    30841 cgcatggcgg aaagcaccgg aaaaagtgaa gcattgacgc tccatagcgc gcttggatta
    30901 aataacgatg aggagtgtga ggctgcggac gaaatgttat ccgaggattt catcattgcg
    30961 gatgagttta cgatggctga tatgcggctg tcctatgaac ttttcaagca catcgaaaag
    31021 ggtgtacgcg tggtgatcgt aggagacgta gaccagttac ccagtgtcgg tcccggaaat
    31081 gtgttccgcg agcttgtgct gtgcggcgtg atcccggtca caatccttga catggtattc
    31141 cgtcagggaa aggacagcag gatcgcagca aacgcccata agatgcagga gaatgatacg
    31201 aacctggatt atggagatga ttttatcttc tgtccggcgg atactgcagc ggaagcggca
    31261 gacaaggttg cggaatatta cagatccttt gtggatgcgt atggcgttga caatgtgcag
    31321 gtacttactc catttcgtaa gaagggagaa gcaagcgtta atgccttaaa cgacaggctg
    31381 tgggagatgg taaatccgaa aagcagtatt gccagggaat taaaggccgg caatgcgctg
    31441 taccgggaag gagaccggat tatccataac aaaaataaga atgacatcag taacggtgac
    31501 atcgggtata tcacggatat ctatcaggat gaggatggcg tggatcttat gcgactgtcc
    31561 ttttcggatg gaagaatcgt ggaatacagt gcagaggatg cggaccttat cgaacacgca
    31621 tatgcaacaa ctgtacataa gagccaggga agtgaataca aggttgtaat cttaccgtgg
    31681 cttcccatgt tttatctgat gttgcgccgt aatattctct acacggcgat taccagggca
    31741 aaagagaaaa taattatcgt aggcagcaag aaggctctct accaggcaat ccataacacg
    31801 gcgtgcgaca agcgaaatac ccgtcttgga gagcggatcg taagagaata caacgcattg
    31861 cttacaaaga aagcttcagc ataggaggat gacaatgaat aaatatgcgg atgagtttta
    31921 tgcgacattt caggaacttg cgaggaaata ttcagcagaa aaagtgtggc aagatgtgat
    31981 ttatgcctgt gctttaacgc aatcagtatt tttacgaagt caggataaga gcggtcatga
    32041 aaagattgat aggataatgg agcaatattc cgaagaagag cagtataaaa taatgagttt
    32101 atatgcgaac atcatggatg cactgcaaga aaatccgggg caggattttt taggagagat
    32161 gtatcacagg cttgatttgc aaaagagtga aaaagggcag ttttttactc cgtatgatgt
    32221 atgcaagctg atgactttat taagtttatc caccgagaat cttgcaggag aaatttccca
    32281 aaagggttat gttacagtaa gcgatcctgc gtgtggatcc ggagcccttt tactctcttt
    32341 ttatgaaacg gtaagagaaa tgggaattac aggttatcag gtgttggctg tggcacagga
    32401 cgttgacaga tcagcagctc ttatgtgtta tctgcagctt tcggtgttat ttattccggc
    32461 aatcgtaatt gtcggtaatt ctattaccaa ccctgggata gatcctcaaa atgaggtatg
    32521 gtatacgcct gcttacaatg tatatcactg gcattatgag cgttaccata taggagaagg
    32581 gacggatctt aggattgcag tcaacaagga aagtggagga aaggtatagc atgagcgaaa
    32641 tagtaatgcc ggagtgcatg aaacaaagaa gagcaaagtt attaaatgcc acgtatgaat
    32701 atttccttac tgtacttgag atggacagaa acgaggcagc actgcggttc cccatggatg
    32761 aacagctgat gtgggtactc tttgggtacg cagacaccat gctggaacga ttagggttca
    32821 acgtatgcta tccggacatc tatgtgcagg atgggaagaa gatattatgt aatactacgc
    32881 attgcggatg taagacctgc catatcaggg aagcatccag aatatcagaa cagttaaaag
    32941 atgtaataga aaatgcaggg ttttgcatag tcagcgagga tatcaccggg ataaaagtac
    33001 gggatccaaa ggatgacgaa tgcaccatat ctattacagc aaagaaaaat tcatagaaac
    33061 agatgggaag gcggtattta ttatatatca ccttcccaaa atggaaggag caaaaagaat
    33121 gagtgaacag acctatatgt atgagcagga aacagccgta gcagcattac accaggtgga
    33181 aggattcgat ccgagaaagt ttatgcgcct gatacagaag gaagaccagt catccagata
    33241 ttacttggat gtagcttacc gtaaactgtg gtttcgcctc tgctatccgg aaggaaagat
    33301 cgtgaaaacg atccgtagca tcacggagaa gatggcaatt gtggaagcta aggtgtattt
    33361 aagcaagaat gatccggagg attcctatgt tgccaatgcg tttgcacaga agtaccgcat
    33421 ggatgattca gagatcggtc agaagtatgt ggaacttgcg gagacggcag ctgtgggacg
    33481 ggcattatcg gatgccggtt tcggacttca gtttgcagac cgtgaaaaag agatggatcc
    33541 ggaagtaacg gaggcaccca tcgatgaaga agtgatggca ggaactccca tctgtccaga
    33601 gaacattcca gaggaagttt ttgggagcag tgttggagaa aacctgatgg aagatatgat
    33661 tccgggacag tgtggaattg aggattatat cccgatgccg gatgagatca tgcaggtggc
    33721 ggaggcttca aaagaggaag cacaacagcc caaggcagca cagcagccta cgaagaaacc
    33781 ggagcagaca ccacaggagc agttcatgca aaataatgtg gctccggagg ccacagttgg
    33841 tattacaagg gatatgccgg taaatgacat ctacgggaag ctcaccagag atttagcggt
    33901 aaaggtggta gtttctgccg gatgttacaa gggtaagact ctggggcaga ttgcagtaga
    33961 gaagccggga tccttacagt ggtatgtgga ttcctataaa ggtccagata acctgcttcg
    34021 tgcagcagca aagtatctgc tcgatcaggc acttgcacct gcctgctaaa atatagggcc
    34081 ccacattttg tggggtcgat agaaaggaga ggtacagcaa tgcaggattt tccatttacg
    34141 attcgtgacg tggcaagtgt gatgaatctg cggatcagac atcggaatac ggtaagcctg
    34201 gatgtagact gtccgctttg tgacgaccat aaagggaaaa tgaatctgaa ctttcagaag
    34261 aatgtattcc gttgtaaccg gtgcggtgag agcggaggaa tgctaaatct gtatgcgaaa
    34321 gcatacgggg tagatcgcca gaccgccaga aaagagatta tcgaagctac cggtggaagt
    34381 gcatttaaga gagagcagat ccatagaaga gagatagaaa gcaccagacc acagatcact
    34441 aatgcagaaa tggcagaaga tgccgtaaag cataagactt ataccaggct gtttgagatg
    34501 ctgatcctgg cagactgcca caaagacagt ctgttaaagc gtggattcac ggaagaacag
    34561 attgaagcaa atgggtataa gagtactccg gtatatggct ataaaaagct taccaagcgg
    34621 ttgatcgagg aaggatgcac ggtagaaggc gttccgggat tttacaggga taaggacgga
    34681 gaatggacga tctattttaa cagaaaagct tccggtttta tgatcccggt gaaaaatccg
    34741 gatggactca ttaccggcgt acagattaga ttagatcacc cttacgatgg cagaaagtat
    34801 atctggttgt ccagcgtcaa ctttgaagca gggacgactt ccggaagccc tgtgcatttc
    34861 gtgggaaagc cgggagataa gacggtattt gttacggaag gaccattaaa aggagatctg
    34921 gcccatgcgt tatccggaag aaccttcctg tgtgttcccg gagtcaacca gtcagcaaac
    34981 ctgatgccag tcttaaatga gatgaaagag ctgggaaccg ggttcctcta tgaagcctat
    35041 gacatggaca agctgcttcg accggtctgc cagggggatt acagtgaaaa ctgtaaggaa
    35101 tgtccctgtt atcgtaagga ctggaagaag cagcgtattc cctgtgaaaa aaagcagatc
    35161 aagcgggata atatcaaccg tggctgcaat aagcttgccg agatctgcaa ggagcttggc
    35221 cttcagggta agacgcttac ctgggatacg gatacagacg gtaactgggc agaaaacgtg
    35281 aaaggcgtgg atgattacct ggtttccatt agtaagtcca aatttaggga aatttaattg
    35341 ccctatccct tttgtattgg aaaggaagta gacaggatga aaatggtgta tatagtgatc
    35401 tgctgttgct gcaatgggac gaatattttg aaattgattc tgtttggaca tccgaaaaga
    35461 aggcagaaaa gagatgcaac gaattaaatg aaaaaggtga gaaatatctg atagaaaaat
    35521 gtggatgcgg attatacgaa gtacggagcg aaattgtatg cacgtaaagc aaatccatgg
    35581 tacagaaaaa aggaaatgtg acaggcgaat tatacttacg gtatatttac acagtaagta
    35641 aagtagggta tatttatatc aagtgtaatt cctgtcacgt gcacaacagg taattggttg
    35701 cggtgggtaa cggacccatg caggaaccct gtggcactca aagttagaaa aaagagctat
    35761 tttcatggac gtttcggacg ttagccaaag gatctgatgc atcagaggca ctaggcacat
    35821 gataggctct ttttttatgg gtattattaa aattatcctg cggagtgaac ttcgtcagaa
    35881 tcttcacttt taaagagcac gtcaaacatc ctatcaacgg aagtgaagga tggatattgt
    35941 tcaggatgct tacgcatttc gtctacttct ttcatggcag ctattgtctc atcattgggt
    36001 aggttattta cttcaggtaa ggcgtagctg aaaatgcacc gtccgatact gttcaatgag
    36061 ctggcgctat tcgacaagct ctttgagcat tgcgccgaga ttgacgaagc ggcacgaagc
    36121 cgcatggaac tgatcgtgcc agagctggca aagcagtacg gcgtgaccga aaggctgaaa
    36181 gccgaaaacc aaatggaat
//
LOCUS       DAWEJW010000014        36012 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_523_length_36012_cov_59.733432, whole genome shotgun sequence.
ACCESSION   DAWEJW010000014 DAWEJW010000000
VERSION     DAWEJW010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 36012)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 36012)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36012
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_523_length_36012_cov_59.733432"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    17169..17268
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    27752..27851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    34121..34220
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8899 a         9543 c         7480 g         9790 t
ORIGIN      
        1 gatacgataa tacttaatcc tccccttttg aaaagaggca cccgtgggtg cctctttttt
       61 tgctatcagt tcctacaggg gaacttttcc ttctttgatc agttcaataa aagcatctgc
      121 cacctccgga tcaaactggg ttcctttatt tttctccagt tcccccatag cgtattccac
      181 actcaggggc tccttatatg ctctcttgga gatcatggca tcaaagctgt cgcacaccgc
      241 caggatcctg ccaagaaacg gaatctcttc tcccttgatt cctctgggat aacccttgcc
      301 gtcatatctc tcgtgatggg aaacgactgc ggggatcaca tagttcatat tcggcagata
      361 gtggatcatt tcgatggatt tctccacatg gctcttcatg atctcatatt cttcattcgt
      421 aagctttccg gcctttttca ggatgctctc cgggataccg atcttaccga tatcatgcaa
      481 aaggcctgcc acctttacgg tctggatatc gtttctggac aagcccagct tctgggccag
      541 caataccgca tactgggata cattggtgga gtgctggaaa gtaaaggaat ccttcgcatc
      601 aatagccgcc accagtgcat acaccgtagg cgccacctgc tcgtaaccgg tctccttatc
      661 ctggctctcc tcattgatac cgccttcata tacctggatc cggtccttac cattacgctt
      721 cgcataaaag gccgcccgct tggactgctg gaacaattcc agcgcatccg ctgccagacc
      781 gggataccat gccacaccaa cgctgaatgt aatgggctgc atgacctgtg gtttatttgt
      841 agcattctcc tgtactgccc gggtcagctt ctccgccagc tccaccagtg gttccctgcc
      901 ggtctccggc accgagacca gaaactcatt ggatcctacc cggaatacct ctccgttctc
      961 cacattatcc aaaaatctct tcgcgcacca ttgcagaatc cggtcgccgg tctgttctcc
     1021 gtacaggtca ttgtataact taaaattatc catgtccatg tataaaaagc cccgttcttc
     1081 tcccaaggca ccatcgcggc gcagatacac tccacaatag ctgcggttat acaggccggt
     1141 cagttcgtca tgaatggata cctgatatac cttttcataa gcatcaatgt ttttcagact
     1201 gccggaagca atatttgcca tctgccggat gcagtccgcc tcgcggtaat tgagcttact
     1261 ctcccgcaga cgctccatat atacaaatcc acagaccata ttatcatatt ttaacagggc
     1321 gatctccggt gtctcttttg tctcttttgt ctcttttgtc tcttcctgct ctacgatgcc
     1381 acagactctc tccacctcat cttcgcccag cgggatatgt ccgctctgta ccacctcatg
     1441 gtaaccgtca tcatttatat tcttctcaaa gattctggat tcccagcccg gaattgcatc
     1501 atccagaatg tcatgcatat tggcaaacaa ttccttacgg tttaaaatgg atgccgctct
     1561 gtcctggaat ttccgcagtc cctccagcat atgcttcagc ttctggttca ggatatcctc
     1621 cagacgcttt ctggcaaaag ctaccaccag caccgtccag gcacattgca gaacgatata
     1681 gatcagcagt ttctgcgtta ccgcccgctc cagacttccc agcacatttt ccagattatg
     1741 agacaggatg accaacggca gggatgccac cacaactgcc agaaaatata ctgcacctac
     1801 cgttacacga taggaaaaat caaacagata ctgcttgtac atgatataga ccatgcacac
     1861 cgccatacca acgccgccca gcatatcata cggaaagacg cttcccggaa tcaggcagag
     1921 cagatttccc atcaaaacaa acagggttcc cagcaacagc ggctgtagtt ttttacgcag
     1981 ctgtaggtct ccaccgatct tgcaatgagc cagataagtg gcatagacca ataccgagac
     2041 ctcccatacc gtcaacaccc agattccgga tgttgcatga tacatataga cataagttcc
     2101 atcggcacgg gtcatcactt ccggtgccgg caggatcgca ccactccagg cattgcccag
     2161 aaccagagct gtcgtcagga ctccatagat atagatcagc gcatcatgct ccgtaatttc
     2221 cagaaaagca aatagaaatg catacataaa cactggaatc accagcagac caaacagtgc
     2281 aaaattatgc cagaaacgaa ttcccggaaa tacctgtagc cgcatgagaa tcgccccacc
     2341 ggtccacacc aggcaggaag tcagcactgc cctcagacta cgaactgtcc ggctcttcat
     2401 ggtattgaaa aagcagaata acagaaaaag ggagcacaaa aaggctccca ccggaacacc
     2461 gatgtagaga tttctatcta atgtcatatc cagaaagctc tccatacttg tattactccc
     2521 tcacactttt gtttttattt tcgaaccgat atcccttttt cgacccgaat cctcttatgt
     2581 atgtaaaatt ataccacatt tttcctttaa agacaatggt acgtccaaaa tcctctgatt
     2641 ctctgatccg cgaaaaggta gacgtaaatt acggcactct tccttgtatt ctccgtccac
     2701 gataacatcc agaagcggta aaagtttcgt gatcaccgga tctcccgctt ccatccaggc
     2761 cagaagatcc ttatcaaaat catagccggt atagcaccag atcgtcttcc ggggataaat
     2821 ttcccttact ttctttacca gagaaagcac cgttgcacgg ttctgcggtt ccataggttc
     2881 gccgcccagt aagcttaagc cccggatata gtccggggcc agtgcctgta agatctcctg
     2941 ttctgtttcc agcgtgtaag cctgtccgta ctgaaaatcc caggccacac tgttaaagca
     3001 tcccttgcaa tgatgggtac aaccgctgac aaacagcgat actctcacgc cgggaccgtt
     3061 tgccacatcc gttttcttga tcgtcgcata attcatagga catttccttc ccttttacta
     3121 tctgcatatt acagatgaag aactcttgcc ttgatctcct tggtcttgcc ggcattccag
     3181 aagttctctc ccagataacc gcaggttctt ctggtgacat tcattttcgc atgatccttg
     3241 ttgtgacact gagggcactc ccattccata ttatcattga tcatgatctc accgtcatag
     3301 ccacagacgt ggcaataatc ggatttggtg ttgaactcac cgtactggat attatcgtaa
     3361 ataaaacgga tgacttcctc gatggcttcc acattattgg tcaggttcgg tacttccacg
     3421 taagagatag ctccgcccaa cgacttattc tggaactcac tctcgatctt gaacttggtg
     3481 aaagcgtcga tcttctcccg aacatccaca tgataggaat ttgtgtaata tcccttatcg
     3541 gtgacattgg aaatatcacc gtatttttcc cggtcgatcc ttgcgaaacg ataacacagt
     3601 gtctcagcag gagttccgta gaggctgaat gcaataccgg tctcctcttt ccattttgcg
     3661 cagcgatcct tcatataatc catgacacgc agggcgaatt ccttgccggg ttccacggtc
     3721 tggctgcatc ctttcatcag atacgtagtc tcatacagtc cgatatatcc cagagacatg
     3781 gtggaataac cattgtgcaa gtatttgtcg atggtctcgc ccttcttcag acgggcgatg
     3841 gctccgtatt gccagtggat ggggctcaca tcagatacca caccttccag tgccttgtga
     3901 cggcacatca atgcttcata acacagttcc agacgctcgt ccagcagttt ccagaatttc
     3961 tcttcatttc cttttgccag aataccgatc tgcggaagat tgatgctgac aacgccctga
     4021 ttgaagcgtc cttcccactt gtactctccg ttttcatcct tccagggaga cagaaaagaa
     4081 cgacagccca tgcaggaaaa tacatttccc tcgtaattct cgcgcatctt cttcgcggag
     4141 atatagtcgg gatacattct cttggcagag cactgtgcgg ccagtttggt cacatagtca
     4201 tattttccgc cctttaagca gttgttttca tccaatacat agaccagctt cgggaaagca
     4261 ggagtcacat agacgccctt ctcattcttg gtcccctcga tccgctgacg caggatctcc
     4321 atgatgatct gcacggtctc ttccacatat tcgtcattct catcgatatg caggaaaagt
     4381 gtgacaaaag gagactgtcc gttggtggtc atcagagtat tgatctgata ctggatggtc
     4441 tgcacaccgg atttcagttc gtcatgcagg cgcatctgca cgatcttgtc cttggctgcc
     4501 tgatccagag tatccccgaa ttcttcctcc agctgttttg caaatttttc cttactctta
     4561 cgcagatatt tccccagatg cttgatattg acgctctggc cgccgtactg gttgctggcc
     4621 actgccgcaa tgatctgggt ggtcacggta catgccacct gaaagctctt gggagactcg
     4681 atcatcttgc cgttgatgga agtgccgttg tccagcatgt ccttgatatt gatcagacaa
     4741 cagttgaaga tcggctggat gaaataatcc gcatcatgga aatgcagcac accgttgtcg
     4801 tgcgccatgg caatatgctc cggcaaaagc agacgtctgg taacgtcacg ggacacttca
     4861 cccgcaatat agtctctctg agtggaagcc agacgggtat tcttgttgga attttcctcc
     4921 gccagctcct tattctcatt ccggatcagt tttaaaatag attcatccgt agtattggac
     4981 tttctgagca gacttctctg gtaacggtat acaatatatt tcttggccag ggtatactta
     5041 ttatgctcta ccagatgctc ttcgatcata tcctggatat gctctacggt ctgggtctgc
     5101 ttgccctcct gctgcacatc ctccagaatc tcttcaatca gtccttcgct ggcacgatac
     5161 cgttcctcca cttcgttatt ggccttatgg attgcttccc ggatcttctc ctcttcataa
     5221 ggcactactc tgccgtctct cttctgaatc ttcattgctg tttccctcac tttatatcat
     5281 tcccataaga tacgaattcc tgcataagcc gaaagaaaag ccgcatcgga agcaagttcc
     5341 cgacacgact ttttctgcaa atcatagtgt ctcactagat tttgtgtttt ccaagtaatt
     5401 tcgtatctac atcttgtatc taatattata gtgaggatac atggaaaaag caagtatctt
     5461 ttttaaaaca ttcctcttcc accataaccg ccgaagcata aaccgccacc gccacagcaa
     5521 aggttgcaga acagattggc cagacaaatt ttcaggcaca ggtcgttgcc acccacatgg
     5581 ggatagccat aggctgcctg tctgctctca taccactgtc ccccgctctg tagtctctgc
     5641 aggagcatct ggtattccat attctgggga tccagcgcca ccgccttgtt tgcatgttcc
     5701 agcgctgcta cattatttcc aaggcccatg tgtgccatgg cactgtagta ataccatctg
     5761 gccgtcttct catgttctcc catgccgtcc aacgtatttc tggcctctct gtaatagccg
     5821 tttctgagat aattaccggc tgcccgcagt ctgggctctt cttcatagcc agaagtctgc
     5881 tgttgccagc ctgcctgctg ccagaagtct ccaaatccct gaccgtagcc gccatatccc
     5941 tggcttccac cgtagccgct tccgctacca ccataggtct gcctgccata tccgccaccg
     6001 taactgtagc cttcggtccg ctccttcatg atctgctggt aggctgcctg gatctccttg
     6061 aacttttctt ccgcctgatc cttattggga ttgttgatat tggcatcggg atggtatttc
     6121 cggctcagtg ccttatatgc tttttttatt tcctcctcgg aggcatctct gctcactccc
     6181 agaacactgt atggatcata agtcatttcc cgttacctct tttgcactgt ctttcatccg
     6241 tttcttccgc acgatctcat aacggcacca tacaccggaa tacagaatat tccgcagaat
     6301 atccacattc tgtaaaatag gaagctgttc gaactcctta cagcactccg acatcatcat
     6361 ggtcaggatc tgccggcatt cctcctcaaa gcccggttcc tcataccgtt tctttaacgg
     6421 attataggtt ccttttttga ggtcctgctc gatatcctca taggcatcca acagataaat
     6481 gaattttccc aggaaaaatc caagtctctc cagattgtcc ttccactcat cctcacgaca
     6541 ggatacaatc tgtgccatga ctctgccaaa caatcccgcc atggtatcaa tatccgcatt
     6601 gccctgcttt tccgcatccg caaaatcctg catggccagt gagatatttc ttactttttc
     6661 ctgatataat aacggttttt tacggaattt tcttcccaac agataactgt acaccagtcc
     6721 cttcagacta tgctcgtcct cccagtcgtc ctccgcttta tagcaggaaa aaagcaccgt
     6781 catatctgcc acataatccg taaacagatt tcttcttgtg ggatgttttt ccaaaggatg
     6841 ggccacacat ctgcactggg cctcctgctc ctccggttca tataagccgg tgagcagcat
     6901 caggataaaa gtcatatcgt aattcagaga aagctgcccc aggacaccgt aacgttcctt
     6961 caggctctga cacaatccgc aataatagga tcgataaaca tcatactcct taaatttcat
     7021 ttccgactga ttgatcacaa tatatccgaa catctggctc accacccttt cctatatttc
     7081 tgtattccaa ttttcatttc ccgtgatcct gccacaggat tttcccgtca ggtcttgcgg
     7141 ataaattctt caccgcattt tctgcaccgg attgcgatct tccccttatg tctgggtacc
     7201 cgaatcttct gtccgcattt cgggcattta tagatcttgt actccttacg accggcaata
     7261 gcctttttct ttcgctgcca ccatccgcga accttcattt ctttcttcag ataccactgg
     7321 ttctcggcac tcctcttact caggtcttta gaaaacatcc ggtaatacgc atagactaac
     7381 agggccagag ccagcacata aaatattctc agaccaaaaa aagagagcgc caggcagacc
     7441 agagccacaa tcatcaaaaa ctgattgaag cggtcgttgc cataacgccc ccacatgaaa
     7501 cgctgtaact tctctttcat gaagtaccac ctttcgtccg ccaccataca ggggacacat
     7561 tatccaaatc tttcagagtc tccttcacga agtctcctct gaatgttacc tacattatgg
     7621 tatgccgaaa ggcaaaaagc aattaactaa gtataaactt ttgtaaaaga actattattt
     7681 attccacagt tcctgggccc gttcatccca gtttttcgcg tattctgcgc actttccgca
     7741 cagatgctcc acagactccg gtgactgtaa atcagtggaa tgtgctccgg tctcatgcac
     7801 catacgctgt aagatttccg gattttccag catcgggcag ggctttaagt gattgcagtt
     7861 gaaaggctgg ttcgcacggt atgccataaa cagtggctgc ttcaacgcag tcagcagatc
     7921 tacttccttg atattggcac cggaataatg gatgaataca cagggctcca catcgccgtt
     7981 tggattgata tggcagtagt ttctgccgcc tgcgatacat ccgcccacat attctccatc
     8041 gttctggaaa tcgaaggcaa agataggttt tccgcccttc atggcacgga tttcacgaat
     8101 acggtgatac atatattctc tctggtccgg atctaagagc aattcggtag atgcatcatt
     8161 gcctacgggc atataatgga aataccagct gaatctggca cctttttcaa tgatcatatc
     8221 caggaattca tcactggtga ctactttata gttcttgctg gtatagcaga tggaagttcc
     8281 gaagatcaga ccgtactgct tcatcagatc catggcatgc attactttat caaacacgcc
     8341 cttgccgcgg cgtaagtcgt tcacttcgtc gaagccttcc agactgatag ctacggagaa
     8401 gttgcctacc cgcttcatct cctggcagaa tttctcatcg atcagcgtgc cgttggtaaa
     8461 tgcatggaag ccacagtcat cgtgtttctc gcacagacgg atgatatcat cctttctgac
     8521 cagaggttcg ccaccggtga agaaatacag atagatacca agttccttac cctgggtaac
     8581 gatgcgatcc atatcttcaa agcttaagtt cagacgattg ccgtactctg ccgcccagca
     8641 gccggtacaa tgcaggttgc aggcactggt gggatccatc aggatcagcc aggggatatt
     8701 gcactgatgt ttcacacgca tctcgttgat ctctttttta ccgcagaacg ccgcctcaaa
     8761 accatagtcc agtgcggaca ttttgatgac atggggatcc acttcgttta agatccggtt
     8821 cacatactgc atccatttgc tgtccggatc ctggatcata cgtcttgccg catcatagct
     8881 ttccttgcgg aaggtatctc ccataaattt ttccgacaga tctaccagct ttaagaatgc
     8941 cttttctctg tctgcttcgt ccttattcac atacttcaga attccgtcga ttgctgcgcc
     9001 aaatgcagct ctctcaactt tgtgtttcac gtctgccata cgatttacct cacttttgtc
     9061 tatttattct atgaatcacg gtatttcaga tataatatcg aaacaataca tccacgccgg
     9121 caagcaggaa gatcagcgct ccactgatca gtacacggtt tcttccatga cacccgatcc
     9181 gatatcccgc atagatgccg gcgatgaccg ccagcgcagc cataattgcc agctgaatga
     9241 tctgcgccgt tactctggtg tctaaaaagc ccatgccaat tcccgtcaga aaactgtcca
     9301 cactggtaag catggcccaa atgcatagct gtctccaggc gaaggcatct tcccttcgtt
     9361 ccagcgggtc ccgcatatgt agtgcccgga cgatcagcca tatcccgatc aggaaaaaaa
     9421 taactgccga tgccgccctg aacagtcttt ctgtccgctg atagttcatt gcaatgtacg
     9481 gaagttccga aatcaggctt cctaaaagca ttgcaccgct ctgccatcct gtgaacagca
     9541 ggatcatttt caggatctgc tttttgtcca gtctggaaag catagcgcct ttgtataatg
     9601 cataggtaaa gacatccagg gatagtccca ccgatacaat gaaaatttca taccatgcca
     9661 tccatgcaac ccctttctcc cgttataatg tttttatctc ttttcatttt ttactttaaa
     9721 caattttccg gcatatggta agatacaacc cttgcacagc gtttttcctg ctcttacatt
     9781 ttttcgataa ctgtaaatat tccgcgacat tttggcaaaa gtgtcgttta aagttgagaa
     9841 aagaaagttt tttggttata atatttctat cccggttctt ttataggaaa aggagtcctg
     9901 tgatgaagac aaaagaatta ttggcagaca gttttcgttc tcttgtgctt accatgcctt
     9961 ttcagaagat ttccatcaaa atgatcacgg atggtgcaca tgtgatccgc cctactttct
    10021 ataactattt tcaggataaa tacgaagtga ttgagtttct gttcgaccag gacatcggca
    10081 gcaaagtcga agtcatgatc gaaaacgata tggaacagga agccatcaag ctgatgttta
    10141 tctgttttga aaaaaataag gaatattatc atcgactttt tgaaaccaca ggccagaatt
    10201 gcttcggaga atttttttca cactactgtg aacagacctt tttccggatt ctgtccagac
    10261 atcctatgag gaaaatgccc tgcgaactta tcacaccgca gtcactggca cattataatg
    10321 ccctcttatt aattgaaatt ctaaagttat ggctctcctc tccgaaagaa gtccctgcgg
    10381 agaaaatttt tgaggcctac caatatatta tacgaactcc gatactggat atgatccaat
    10441 atccggaacc ataaaaagac aggtcgaact gacctgtctt tttatatctg ataaacttat
    10501 aatcttgtga tcacaaagat atcatagatt gcctgaattg cggtctcaaa atcttcattt
    10561 gccacaccga tgatgatgtt cagttcggaa gatccctggt cgatcatctt aacattgata
    10621 tttttgtgtg ccagtgcgga gaagattctg ccggcagtgc cgcgggtgga cttcatgcca
    10681 cggcctacga cagcgatcaa tgccaggtcg gattcgatgt caatggtgtc gggattggcc
    10741 agtctgtgga tgcctgccac taccttctgt tccttgtgca tgaactcatc ctgatgtacg
    10801 aataccgtca tggtatcaat gccggaggga acatgctcga aggaaatacc attttcttca
    10861 aatgcctgca atacctttct gccgaaaccg atctcggagt tcatcatatc tttttcaata
    10921 ttgacggagc agaagccctt cttgccggcg ataccggtga tcacgtattt ggacttctga
    10981 caggtgcttc ccacgatcca ggtaccattg tcctcgggag cgtttgtatt gcggatattg
    11041 atagggatac cttcctgacg taccgggaaa atggcatcct catgaagaac ggtagcaccc
    11101 atataggcca gctcgcgaag ttcacgatag gtgatagtct caatagcctc gggatgatca
    11161 atgatgcgtg gatccgcaat caggaaaccg gatacatcgg tccagttctc atagacatcc
    11221 gctttggcag ccttggccac gatggaaccg gtgatgtcgg aaccgcctct ggagaaagtc
    11281 ttcaccgttc cgtcgggcat ggcaccgtag aagccgggta caactgcttc cttactctct
    11341 gccagtctgg cacgcagtac ttcattggtc tttgctgcgt caaattcgcc gttctcatcg
    11401 aagaagatga cggttgccgc atcaatgaaa tcatagccca gataatttgc catgataata
    11461 ccgttcagat attcaccacg ggaagccgcg taattgctgc cggcctttgc cttaaagttc
    11521 ttctcaatag tcttgaactc atcgacaaga tccagatcca gctgcagtcc gtcgatgatc
    11581 tcctgataac gggctttgat agcatccagc tctgccttga agctcttgcc ctgatctgcc
    11641 agatcatagc aggcatacaa catatccgtt accttggtgt catcggaaaa tcttttcccc
    11701 ggtgcggaag gtaccacata ttttctctcc ttatctgccc ggatgatgtt tcctactttt
    11761 ttgaactgct ctgcgcttgc cagagaactt ccaccgaatt tcactacttt tgacataaca
    11821 ttttctcctc aactctattt aatgatgtcg ggatcaaaag ccccgacttt tataccagac
    11881 ggatcctgcc gagcatctgc ccgcccagtg cctcgtattt tgcctgaaaa tctttttcct
    11941 tcatactctg tgtgaagtat ccgaagtctt ccttccggtc cttaagatgt accatccggc
    12001 ctccggggaa cgctgctgta accgcatctt ccgctccggc ctttgcccgg atgaagaaac
    12061 ttctctcggc ctcgccgatg tctgccagct ttacttcctc tttgtcccag aagcacatga
    12121 tcacctttcc ctgatgtctt gcacagtcga cgacatccga tactaccgcg ctggcggtgg
    12181 gaagcttacc tgcgccccgt ccgtaataca tggaatctcc cagcatgttg ccggtgacaa
    12241 atacagcatt gaatacacca tgtaccatgg caagcggatg ttctttggaa agtaaaaacg
    12301 gtgctaccat aacctctgcg gtattatcat caatggccct gcttctgccc aatagcttga
    12361 tggtatgatc tgtggcattg gcatattcga aatccgctgc tgtgatctta gtgatacctt
    12421 cggtgggaac cttctcggaa tccacatttt tactgaacat cagggaagac agaattgcaa
    12481 tcttacggca ggcatcataa ccttccacat ccgcttccgg attgcgctcc gcatagcctt
    12541 tgtcctgcgc ttccttcaga accgcagcaa agtccgcacc ctctttttcc attttactta
    12601 agatataatt cgtagtaccg tttaagatac cgttgatagc ctcgatcttc tctgctgtca
    12661 gggaataatt caacggacgg ataatgggaa taccgccgcc cacactggct tcgaacagat
    12721 agttgcaatt atgttctctg gcgatctgca gcagttcggg accgtgcttg gctaccagct
    12781 ccttgttgga ggtgcagacg ctcttgcccc gttccagagc ctgcttcgtg aactgataag
    12841 cggctccgac accgcccatg gtctcacaga caatggtcac actgtcgtca tgtaagatcg
    12901 tattgaaatc atgaatgact ttgtcctcgt aaggatctcc ggggaaatct ctcagatcca
    12961 ggatgtattt tacctccagt tcctgtgcgg acttcttgtt caccatgtcc ttgtttttct
    13021 cgataacttc caccacaccg ctgcccacgg tgccgtagcc taatactgca acgttgatca
    13081 tatcataacc cttgcctttc tcatagcctg caaactttca ggtactttag taccttgcgc
    13141 aggcacaaat ttgcgtgtga aaaattttat tcttttgcca gtatttttac atgatgtaca
    13201 ctgggctgtc cctccagctg ctcgatcatc cgggaaatgt cccctgtggt ctgcaatacc
    13261 tgaatactca atgtcagaga agcgattccg ttgatgggaa tactctgatg gatcgtcagg
    13321 atattggccc ggtactctgc gatgatcttc agcacatccg acaatatccc cggttcatca
    13381 tccatctgaa aggtaagagt gatggtcgtt ccctgggaat tgtcatgaaa gggaaaaata
    13441 tcatctttgt acttataaaa agaactgcgg ctgataccta ccgcatcaac cgcttcctgt
    13501 atggtcagaa ccttctctga ctccagaagc cgctttgctt ccactacctt taacagtact
    13561 tcgggaattg ctttctgctt caccacaaaa tacttggtcg tttctcccat atgttatcct
    13621 ttcttccgtt cgtatctgta gtgcggacac ttgtttttca ccgagggata ttctagcaca
    13681 gtaaagcatg aaagacaacc cccattttta accttttgct gtaaattcca aaaagttcaa
    13741 aacaggggtt gtctctaatt cttgcgggga tatttgttcc ccgtctcccg atatgcgtta
    13801 aaatcccatg ccggtcccgt ctgtaaccat tcatacatga cgtgttctgt attctcataa
    13861 aaaatgtacc ggcgttctct cttttctccc tcctgatatg ggaaaatcgc atctcccgcc
    13921 gctccgtcca tcagtctcac atatgcatcc tgaaggtcct cgtaagctgc aggaacaagc
    13981 gatgtctgta acggatggct ctctcccaca ccttttacca ggaacagagg attctgtctg
    14041 tcatatgccg agacctcatt ctgaggatca aagccatgat ctgccataac gataataacc
    14101 gaattgtcat acagatctga ctgtttcagc ttatcaagga actgaccgat caccctgata
    14161 tttgcctcca gcgtctccgt atacgaagaa tctcctaccg cattcagatc cgcatcatac
    14221 aggaaaggaa catgccctcc ctgcaaatga taataccgga aacactttcc ctcctgatac
    14281 gtcacctttt cttcctgcat atgtgtggca aattctttga gatcccatgt atacagagga
    14341 ttgctctcgt ccacaaaggc attgtgctgc ccctgcgtct gtacaatggc ccgataacag
    14401 tattccttta ggaaaaacgg tgcatagcgg atgccgccca gcttgcagat tgcccgcctg
    14461 aaatacttcc agacatacgc ctcatgctgc agttccacca tattggaaaa ctgataggta
    14521 tacgaagtca cccacggatc agactcatac aatgcgatgt cataatccct ctcagacagt
    14581 tcccggaaca atcctgattc atccacagct gccgccgcgt aatccagata atattcttct
    14641 ccttcatacc atttgcccga taagatatac ggaacagaaa acgccgtcca gggatatgct
    14701 cccaccatat ccggataata agtaaaatca gcaaatgtct ctgtataggc cggatccgaa
    14761 tccatcactt cttcaaaagt cttcgcatcc accgcatcca acagcaatac caggaaattc
    14821 tgatcctgtg acagacggta ctgtccgttc tccagtgcat aataagtggt tctctcccgg
    14881 taagcccctg tggtcacagt catcgtcaca agcgttatca tgaggattcc cgaaagtccc
    14941 gcactcacag ccatacagat tccctgaaat ctctttgcac caagcagctt tgccacggtc
    15001 acgaccgctg ccgctatgag aatacacact atcgctgtct ttatattttc tccccggtag
    15061 tcctcccata cgatttccgt gccgtcgaaa ggcggcatat gattcaccag aaaattgccc
    15121 tgcacatata aagtaagcaa aaccaccagt tccgctgccg taacgaaccg gcaaaaaact
    15181 ttggagatac aggctgccaa aaggattccc aacacattca gtccaaaaaa aagtgcaaaa
    15241 ttctgcagaa ccgcttttaa tatcttataa aaatcaaacc agaattctgt ctggttcgta
    15301 acatacaatt ccaacggtgc ataaataaaa aaaataaaac agacagcaaa agctgccagc
    15361 agtcccggcg ctgccacctt ccacctgtcc gtcacaaagg ttttccattt ctcttttttc
    15421 atctgttccc tctatctgct gttcttttgt attccgtcca acattttaac acgacgactg
    15481 tataaactca actccaacat aaataaaggc cagcctgcac atttacatgt acgggctgac
    15541 ctttgcaaac agcttctgcc tagatcagag gatatttgtc tgttaatttc tgaatgatgg
    15601 ctctggctgc gggaacctgt tcctcgccac ccttgatgac catggcaatg gcctctgcca
    15661 cctgatccat atcctcggtg ttcagacctc tggaagttac cgcaggggtt cccagacgga
    15721 taccactggt cacgaacgga gactggggat cgttggggat ggtgttcttg ttggcggtga
    15781 tgtgagctgc atccaacagc ttctccactg ccttaccggt cagaccgtaa ggggtcaggt
    15841 ctaccagcat cagatgattg tcggtaccgc cggagacaat cttaatgtta cggctcatca
    15901 ggcccttgca caatgcctgt gcgttgtcca caatgttctt ctgatatacc ttaaagtcgt
    15961 cgcttaatgc ttcctcgaag cagacagcct tggctgcgat gacatgcatc agcgggccgc
    16021 cctgaatgcc ggggaaaata gctttattaa aattgaattt ctcgttggct tccttgttgc
    16081 agaggatcat accgcctctg ggtccgcgca gtgtcttgtg ggtggtggtg gtcactacat
    16141 ccgcataggg aatgggactg ggatgaaggc ctgctgccac aaggcctgcg atatgtgcca
    16201 tatctaccat cagtaccgca ccgacttcat ccgctacttc acggaacttt ttgaaatcaa
    16261 tggtacgtgc atatgcagaa gcacctgcaa tgatcatctt gggtctgcat tccagggcca
    16321 gctctctcat tctgtcataa tccaggaaac cgttgtcatc cacgccgtaa ggtactacat
    16381 ggaaatattt accggacata ttgacgggtg aaccatgggt cagatgtccg ccgtgatcca
    16441 gattcatacc cataatggta tcgccgggct tcagcacagc gaactgcact gccatatttg
    16501 cctgtgctcc ggaatggggc tgtacattgg cataatcaca tccaaacagc ctcttggcac
    16561 gttcgatcgc tagattctcg atgacatcca cgcactcaca accaccgtaa tatctcttgc
    16621 cgggatatcc ttccgcatat ttattggtca gtggacttcc catagctgcc ataacagcct
    16681 tacttaccca gttttcagat gcgatcagct cgatgtgact gttctgacga ttctcctcgt
    16741 caataatggc ctgtgccact tcgggatcta ctttttttac ttcatccaat gaatacatgc
    16801 ctcattcctc cttatgttac gttaattcgc taaacttatg gtttagtata gcatagaacc
    16861 ttttatttgc aaagacagaa aatgaacaat gttggcagaa gccccctggt gtgttagaat
    16921 aaactttaac ataactattc agagcctcat tcgccataaa aattttcaaa attatgctca
    16981 ttcatgcaag catgattcgc aaatttatgc aaattttccc ggctctgaat agttacaact
    17041 ttacgaaaga tggggaatcc tcatgtatca tattattatc aatcccgcct cccgctccgg
    17101 aagaggggca aaaatctggg ccgaacaggt ggagccggct ctgaaagcag gcaataccga
    17161 atatcagann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat ctgggccgaa
    17281 caggtggagc cggctctgaa agcaggcaat accgaatatc agacctattt ttccgggaaa
    17341 gccggagaag tccgacagct ggccgcacag atcaccgcag agcatgctgc agagcctgac
    17401 ttaacgctca tcgttctggg gggtgacggc acgataaacg agacccttca gggcattgca
    17461 gatccttcca aagtcatcct cggctacatt cccaccggct ccagcaatga tctggcccga
    17521 gatctgggaa tttcaaagga tccaaaggct gctctcaatc tgatccttca taataatact
    17581 cccagagcca tggatctggg gagactgacc tatctggacg aagaccagcc ggaagaatcc
    17641 aggctttttg ccgtcagctg cgggatcggc tttgatgccg ccgtctgtgc ggaagccatg
    17701 agttcccgga tcaaggatac catgaaccgg atcggcttag gaaagctgac ctatctgggt
    17761 atcgccttaa aacagctgat cactgcccgc aaggtctcct gcacgctgac actggaggac
    17821 aaggtacatg acaaaaagga atccgtagaa ctcccccgtt ttctgttcgt gacctgtatg
    17881 agtcaccgtt acgagggcgg cggtttcatg ttctgccctc ccgctgtcga tcatgacggg
    17941 gtattggatc tctgttccgt aggcaatatt tccaagggat tggttctgct ggccctgccc
    18001 actgcctttt tcggcaaaca ttattttgtc aaaggcatca atgcccatac cgccacacag
    18061 atgtccataa cagcttccgc tcctctgtgg gtgcatacag acggagaagt gacaagaaag
    18121 agccgcggtt tccgggtgga atgtctcccc ggtgccatcc ggatgatcac accttaaaca
    18181 gatttaacac actctaaaga ttttcttaag aatgcttaag tattccaaaa aagccttgat
    18241 ttttttgttc cgatagctat actaaaattg attttggaac ataggttttt agagaaatga
    18301 ggtttttatg gaaaaattac ggatcttcta tttgaaatat aaacatgcca ttcctcttat
    18361 tatatatggc attatttaca tgacctggtt cgcctggctg gagaaaaata ctaccggaca
    18421 ttaccggatc atacatatgg cggcggatga ttatattcct ttttgtgaag tgttcgtcat
    18481 cccttacttt ttgtggtttg cctatgtatc ggctgtggtc atttatttct ttttcaagga
    18541 caaagaggat tattaccgca cctgtatctt cctcttcacg ggcatgacga ttttcctgat
    18601 cgtatccacc ctgtggccca acggacagca ccttcgtccc gctgttatgc cgagagacaa
    18661 tatcttcacc cgtatggtag ccgctctgta tcggacagac actcctacca acctgtggcc
    18721 cagcattcat gtatataatt ctctgggtgc acactttgcc attatccgca gtaaatgctt
    18781 tgagaagaaa aaaggcatcc gcatcggttc cctgatcctg gctgtctcca ttattatggc
    18841 aaccatgttc atcaaacagc attccgtatt tgatgtgctt actgctttcg tcatggcggc
    18901 tgtgatgtac acgctggtat atcgttatga tctgctgatc gctgtcagag agttccgcaa
    18961 taagagaaaa gcaaaaccgc agatcggcta aaggttgttt caaaacgtgc gccgacggcg
    19021 cagctataaa aatgcagtga cgttacccac gccactgcat ttcttttgcc ctcatttatg
    19081 atctgttaca ttacaatact tcttcgctct tcttcaatgc cgaggcaatg accgcatcca
    19141 tatcatagta gcggtattcc gccagtcttc cgccgaagat cacattcttt tccgtatctg
    19201 ccagcctttt atactctgca tacaaagcgc cgttcttttc atcattgact ggataataag
    19261 gctcatctcc cggcttccat tcggaactgt actctctgct gatgaccgtc ttcgggagat
    19321 cctgtccctg ttcgtccttg ccaaactcga accatttgtg ctcgatgatc cgggtccagg
    19381 gagtctcacg atccgtgtaa ttcaccgcag catttccctg gaagttggga atatccagca
    19441 cctctgtctc gaacctgacg gaacgatatt ccagtgctcc cagagagtag ccgaaatagg
    19501 catcgatggg gccggtatag acaacagtct ctgccagttt atccagttcc tctttctgct
    19561 ccagataatc tgtgttcagg cgtacctcaa tgccctctaa cagatgttcc accagcttcg
    19621 tgtaaccgcc aatgggaatg ccctgataca acgcattgaa atagttgttg tcaaaagtca
    19681 gacgcaccgg cagacgtttg ataatgaagg ccggaagatc tttacagtca cgtccccact
    19741 gcttctcggt gtagcccttt accagttttt caaaaatatc cttacccacc agagagatgg
    19801 cctgttcctc cagatttttc ggttccgtga tcccggcagc ttgccgctgc tcttcaatct
    19861 tggccgcagc ttcttcggga gtcaccacgc cccacatttt attaaaagtg tacatgttaa
    19921 aaggcagtga gtacaactct ccgtgataat tggctaccgg agaattggtg aaacggttga
    19981 actccgcaaa ttgcgtaatg tatttccaca ccttcgtatc attggtatga aaaatatggg
    20041 caccgtattt gtggacatga atcccttcta ccgtttccgt ataaatgttt cccgccacat
    20101 tagggcgttt gtcaatgacc agaacctttt tccctctgtc ggccgcttct cttgcgaaca
    20161 ccgctccgta caggccggaa ccaaccacca gataatcgta tttcataagt tctacatatt
    20221 tcctttctca cagtcgcatt cctgcgctta cgttatttcc gtacgacagc cttctctccg
    20281 aagacacgtt tcagaaccac cagagtagcc agtgtcagca gaataccgat caccagacag
    20341 atcaccacat tctgtacaaa acctgcaatg gtataagtca caaaagagat tgccgccacg
    20401 gtcagtacat aaggtacctg ggtggataca tggttgatat gattacactg tgctcccgca
    20461 gaagccataa tagtcgtatc ggagatgggg gaacaatggt cacccataac agcacctgcc
    20521 agacatgcag acacaccgat gatgatcaga tgctcggctc cgggtacgat agctccggtc
    20581 gtgggatcgg taaatacgcc ggtaacgata gggatcagaa taccgaaggt tccccagctg
    20641 gtaccggaag caaaggccag gaaacatgct accaggaaaa tgatggccgg aagcatattg
    20701 gtaagccccg tggatgctcc ctgcatcagg cctgccacat agacatccgc acctaacagt
    20761 ccggtcatgg tcttcagagt aacggcaaag gtcaggatca gcatggcagg aaccatggca
    20821 atgaagccct gggtcagaca gttcatggcc tccttgaagg ataccagtct tctgcacata
    20881 tagtatacaa tggcaaataa taatgcgatc aaagatcccc agggcagtcc cacggaagcg
    20941 tccgttgcgc tgaatgcatc gataaagccc acgccttcaa agaagccgcc gacatagatc
    21001 atgccgatga cacagcagac aatcagtacg atcaccggga tcaacagatc gtagaccttg
    21061 cctctggtgg aacctttggg tgcattgtct gcattggcca cgcgctctcc ctcggtaaac
    21121 agatcaccct tttctctggc attgcgctca tgctttgcca tggggccgta gtcgaagccc
    21181 atcagcgcca taccgacgac gaatacgatg gtcagcagag aataatagtt ataaggaata
    21241 gctcttacga acagttcaat accggaataa acaccttcat ccaccatacc ggatactgcg
    21301 gccgcccagg aagagatcgg tgcgatcata cagatgggtg ctgcggtggc atcaataatg
    21361 tatgccagct ttgctcggga aatcttatgt ccgtctgtta ccggcatcat aacagagcct
    21421 acagtcagac agttaaaata gtcatccacg aaaatcagaa cacctaataa aaaggtggaa
    21481 agcagcgctc ccgctctggt ctttacatgc tttttcgccc attcgccgaa ggctgcagag
    21541 cctcctgcct tgttcatcag tgctaccatg gtacccaaca ggatcaggaa catgaagata
    21601 ccgatattcc atgcatccgc cacggaattg ataaatcctt cgttgatcac cgtatccagc
    21661 gttgcaatag gagaaaatcc ggtataaatc aaagctcccg taacaatgcc gataaacagt
    21721 gacgaatata cttcttttgt gatcagtgcc aatacgattg ccaccacagg cggcagcaat
    21781 gcccaaattg ttccgtacat aattttgccc tctctcgtac tttttttgta acatttcaga
    21841 tacattttta ctttatggca aacccgtata cctgtcaata taattttcca aattaacgct
    21901 ttagcacagc aatttatccg ttccttttct ctttttcgct ttaaatcgct aaattttctt
    21961 gcatccttgc acgatctggg ctataatgag caatgacatt tagcagaaag gacgtgccct
    22021 gcgcacgatg gaataccatg agtttcgaat ttatcaaaaa actgcccact cctgcggaga
    22081 tccgcaagga actgcccctt ccggcagaat tagccaggat caaagaagaa agagatgctg
    22141 aaatccgtga cgtcctcacc ggcaaaagca ataagtttct ggtgattatc ggaccctgct
    22201 ccgctgataa cgaagattcc gtatgtgatt acgtcaatcg tcttgccaaa gtgaatgaga
    22261 aagttaagga caagctgatc ctggtgccca gaatctacac caacaagccc cgcaccaccg
    22321 gcgaaggcta caagggtatc accagccagc ccgatcccga gaagaaaccg gatttcctgg
    22381 caggtctgat ggccatgcgt aaaatgcaca tccgcgccat caaagagacc ggtctcactg
    22441 ccgcagatga aatgttatac ccggagaact ggggttatgt atccgatatc ctctcctatg
    22501 tggccatcgg tgcccgttcc gtagaggatc agcagcatcg tctgaccgtc agcggcttcg
    22561 atgtagctgc cggtatgaag aatcctacca gcggagattt ctccgtaatg ctgaactccg
    22621 tatatgcggc acagcacccc cactccttca tctataccgg atgggaggtg aatacccacg
    22681 gcaatgatct ggcacacacc gtactgcgcg gtgctaccaa caagcacggt gccaatatcc
    22741 ccaactacca ttacgaggat ctgatccgtc tgtgggatat gtatgaaaaa atggatctga
    22801 agaatcctgc ctgcgtgatc gatgcgaacc actccaactc caacaagcag ttcaaggagc
    22861 agatccgtat caccaaggaa gtcatgcaca gccgtaaact cagccccgat ctgcacaagc
    22921 tggtcaaggg cgtcatgatc gagagctaca tcgaggaagg caaccagaag gtgggtgccg
    22981 gctgctacgg caagtccatc accgatccct gtctgggctg ggaagacagt gagcgtctga
    23041 tctacgatat cgctgagtac gaataaaaag aacgattttg caaatggggt gctgaatagt
    23101 taaaacagtt tcaaaaaaac tgcaggaatc cttttaataa cgcaggattc acaaaacatt
    23161 ccaataaaca tccgaaaacc agtgcgatcc atatgagcgc cagttttccc aatctgccgg
    23221 gcagggattt ttttccctgc ccggttgtgt gatggtaaaa ataaatactc cggtgcatat
    23281 cttcacacca tctgagtaac aggatcatag ccggcaggta acacagataa tggggaaaga
    23341 tgctcacgat ccccagaagg atccccttga ttccatacct aaacactgcc gtactcagaa
    23401 aaaagcccac agagaatccg tattttacgg tgactcctcc gcagaaaatc catcccagat
    23461 aagtcgttgc catgataata agcaccagaa aattccgaca tcttttgcac agcacgtacc
    23521 agaacaggat actgctgtcc acggtcatat atttcatgcg atataacgtg tcctcatcca
    23581 gcattcccgc acggtttaac agaattcccc gtccgaaata caccgcaaac aatcccacca
    23641 caaatcccgt caaaaacaaa aagtgcagcg ggagttttcc cgcaggcaac atggatctgt
    23701 tccatttcat atattctcgt ctcccgtcag gcgttgttag taatatatgc ggacaacatg
    23761 gaaaatcatg ctttcttatt cgtctttcag aaaagaaaaa gcccatccgg acaggtactg
    23821 cctgtctgga tggggttccc taaaatcatt atgtagcagc cggtcgtgac tgcttttgtc
    23881 tgtaacggaa ttactcactc aggtatttgg acgcgtaagt taaaatgccg cagattgtct
    23941 tggcatcctg gatcctgcag tcgtagatca tctgctttaa ttcttctacg gaataagcct
    24001 ctacattgat gaattcatct tcatccagat gctggtttcc tctgtctttc agtcctctgg
    24061 ccagataaat atcgatcttc tcattgcaga atgccactgt ggtataaata gaggtcaaca
    24121 gttccacatg gtcagtatga tacccggtct cttcctgcaa ttcccgcata gctgcctgat
    24181 ccgtgggctc ttctctcccg ttcagtccgc ctgccggaat ctccagagtc tcacgctcca
    24241 gggcattgcg gtactgacgc accatcagaa gcttaccgtc ttctctcacg gctactactg
    24301 cagccgcccc tttatggtcg atcagatccc attttgctat attaccgttg ggaatctgca
    24361 tggtatcctg ataatagtcg ataatagcac cctttgctac cagatcacgg ctgatacgct
    24421 tgaattcttc catgacttat catcccttct catccaaatc gaatacttac ctgctttcca
    24481 gtaatttatc tacgctcacc atcttgacgc agagtacaaa cagatccgta gccattgcca
    24541 tctcctccgg tgtggggaag gaaggtgcaa tacggatatt ggaatccttg ggatccttct
    24601 tgtaggggaa cgtagcgcct gctccggtca gagtcacgcc tgcttcctta cactttgcca
    24661 cgatagcctt ggcacagcct tccagggcat caaaggagat gaaatatcca ccgttgggct
    24721 tagtccaggt accgatgcct aagcctccca gctcattctc cagcacttgc agtacagcct
    24781 cgaacttggg acgcatgatc gctgcgtgct tcttcatatg ttcacgtaca ccattgatat
    24841 ccttgaaata acgtacatgg cgtaactggt tgatcttatc ataaccaatc gtctggatcg
    24901 tcatggactt gcgcatatca tccaggtttg ccttggaaga tgcaatagcc gcaatgccgg
    24961 ctcctgcgaa ggttaccttg gaggtggaac agaactcgaa taccatgtcc ggatgtcctg
    25021 ctttctcaca ctcggaaaga atctctaaca gctggtcctg tctgtcatca tataaatgat
    25081 ggatggcata ggcattatcc cagaaaatac ggaaatccgg tgcggcaggc tgcaatgctg
    25141 cgaaacgtct tacagtctca tcggagtagg aatagccctg agggttggag tatttcggta
    25201 cacaccagat acccttgatg gcaggatctt cagacaccag cttctctacc atgtccatat
    25261 ccggtccttc gggtgtcatg ggcacattga tcatttcaat tccgaaatgc tctgtgattc
    25321 caaaatgtct gtcataaccg ggtacgggac acagaaactt tacatgatcc agcttacacc
    25381 agggggtatt gcctaagatg ccatgggtca tagcccggga tacggtatca tacatgatgg
    25441 tcaggctggc attaccgcag acgatcacat tctccggctt cacttccaaa atgtcagcga
    25501 tcagtttctt tgcctcagga ataccgtcca ggccaccata atttcgaaca tccattccat
    25561 cctcagcctt catagcgctc tgagaattta caacatcgag ataatccatt gtcatatcca
    25621 gctggttaac tgccggtttt cctctggaca tatccagttt caggcctttg ccttttgcat
    25681 cttcatatgc agcgttcagc tctgccttca atgttaaaag ttcttccttg gataattcct
    25741 gatatgcttt catttcgtac catacctttc tcgttttttc ccatagggaa tacgctttct
    25801 tccgtggacg ttggataatc gtatacaatc atacacttcg aatatcatac tataagaaag
    25861 ttctttgtac aatacctttt ttcgaaaatt ttatcaaaaa agctgctgtg ctttaacagc
    25921 acaacagctt ttcttacttc gtatgaaatt gtcatttctg actggtcagt tatttggcgt
    25981 aatcaattac gcggctctca cggatcacat ttaccttgat ctgtccggga tattccaatt
    26041 cagcttcgat ctgcttggaa atatctcttg ccaacaatac catatcggca tctgaaatct
    26101 gctcgggcac taccataaca cgaacttcac gacctgcctg aatagcaaaa gatttatcta
    26161 cacctttgaa attgtttgtg atatcttcta actgtcttaa tctgctagta taggtttcca
    26221 gtgtttccct tctagctccc ggtcttgcag cggatattgt atcagcagct tgtacaatac
    26281 atgcaatcag ggaagtaggc tctacatcgc catggtgaga ttccacggca ttgataaccg
    26341 tagcgttctc tttatatttc cgacacagtt cagcacccag ctgaatatgg gaaccttcca
    26401 tttcgtggtc tactgcctta ccgatatcat gcagcagtcc cgctctcttg gcaagtctca
    26461 catccagtcc cagctccgct gccagaagac ctgacaactg tgcaacctcg atggaatgct
    26521 ttaatgcgtt ctgaccatag ctggtacgga atttcatctt accgagtaat cttaccagtt
    26581 cgggatgaat gccatgtaca ccaacctcca gagttgcggc ttctccctct tccttgatca
    26641 ttacttcgac ttccttctgc gccttctcta ccatctcttc gattctggcc ggatggatac
    26701 gtccgtccac aatcagcttc tccagcgcaa ttctcgctac ctcacgacga atcggatcaa
    26761 atccggacag gattactgct tccggtgtat catctatgat caggtctaca cctgtcatgg
    26821 tctcaagggt acggatgtta cgtccctcac gtccgatgat acgtcccttc atttcgtcat
    26881 tcggtaactg tacaacggaa attgtagtct cagagacatg gtctgctgca catctctgta
    26941 tagcgttaac cacatattcc tttgcctttt tgtctgcttc ttctttggcc cgactctcca
    27001 tttccttgat catcacggcc gattcatgtt tcacttcatc ttcaacaatt ttcaataagt
    27061 agtcttttgc ttgttcagag gttaatcctg aaattcgttc cagttcctgc acacgctgct
    27121 cattcagtct ggagacttct tccttcatgc gattgaggga ttcttctctg gatgctaaac
    27181 ttgcctcgcg cttctcgatg gcatctgctt tcttatcgat gttttcttcc ttctgctgaa
    27241 ctctgcgctc ataccgctgg gcttccgctc tgcgttcctt gatttctttg tccagttcat
    27301 tcttggtacg aatggactct tccttgactt ccaggagtga ttcgcgcttt ttgttctctg
    27361 cagtcttcag agcttcatca ataatctctc ttgccttctc ctctgcgttg ccgatggttc
    27421 cctccgcgtt cttcttcaga cggttcgtgg taacctttgc tgtgatcagc actgcagcta
    27481 taatggcaat aacagaagcc actaatacta ttacaatatt agtgtccact acaggagcac
    27541 ctccttatga aattttcatt gtacattttg ctccgataat acaaattatc gttcataata
    27601 gaatgcaatt tatgtacatt ctaacaagca attcacaaca gtattagtgt aaactgaaat
    27661 tacacccatg tcaagtacaa gtcgcaatta gtccaaaaat agttcctata tcaaaacaat
    27721 ctttgtgcat tatttcttgc tgttatgcgt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27841 nnnnnnnnnn ncggataaaa gccctgctgt aattttgtcc gcagctgtgc cgtcgtatat
    27901 tctcttccct gcaacaggtt catggcacga agcttcgccc gcttgggaag tacctcctgc
    27961 atgatagtcc gatagtcttc ctcgcttaat tgctgtcctt cctttatatg gtaaagacgc
    28021 aattcgcctt tgtacaatac aaaggcgaat tcctgatcaa tatatacttt gcttctcgat
    28081 ttggaaagtt ccgtaacctg tgttaccagc atggatctac tcttccttct ctgctgcggc
    28141 agtgacatct gcctttagtc cgtaatgctc tctgaccttc tcggcaatct catcgcagat
    28201 ggcgggattg tcttttaaat actgcttggc attctcacgg ccttgtccga tcttgttgcc
    28261 ctcgtaggca taccaggcac cggactttgc cacaacgtca cagctggctg ccagatccag
    28321 aatatctcct accatggaaa taccttcacc gaacatgata tcaaactctg cttccttgaa
    28381 aggaggtgca atcttattct taacgacttt gacacgggta cggttaccga tcacttcacc
    28441 gccctgcttc agagcttcga tccttctgac atccagtcgt acagaagaat agaacttcag
    28501 cgcacgtccg ccagtagtgg tctcaggatt accgaacatt acacctacct tctcacgcag
    28561 ctggttgata aagataaccg tacagttgga cttgctgatc acagcggtca gcttgcgcag
    28621 cgcctgggac atcagtcgtg cctgtaaacc cacatggctg tctcccatat caccgtcaat
    28681 ctcggccttg ggtaccagtg cggctacgga gtccactacg ataatgtcaa tggctccgga
    28741 gcgtaccatg gtctccgtga tctccagtgc ctgctcaccg ttatccggct gggaaatgta
    28801 caggttgtca atatctacgc cgatgttctt cgcataaaca ggatccagag catgctccgc
    28861 atcgatgaat ccggcaatac cgcctctttt ctgtacttct gcgatcatat gtaaggtaac
    28921 ggtggtctta ccactggatt ccggtccgta aatctcaatg attcttccct tgggaatacc
    28981 gcccagtcct aacgctatat ccaaacttaa agaaccggtg gggatggtct ccacattcat
    29041 ctgtgccgtg ggatctccca gcttcattac cgcgcccttt ccgtactgct tctcgatctg
    29101 gctcagcgcc gcatccaatg ctttaagctt atcgtctttt tccatgtcaa tctccttttt
    29161 gttcaaggaa caaatgttct gctttttatt taggataaaa catttgttcg tgtttgtcaa
    29221 tgtctatttt tattatacat attttgtgta cattaaagct ccctcataag cattccccct
    29281 tattttttcg ttccgatgat cgtctgaaat tttggcgaat gcctgaacat cctgaaatgt
    29341 ctgtcgataa tcagagataa aagatacaaa aagaatgtat cacacatccc cgcttcccgc
    29401 aaggacgcat gatacatttc ctgtgttttc gtcactatct acaatttttt accccttcgt
    29461 tttgagggta tctcacaaaa tatttttctg ttacttttta ccaaaagtaa ctttactgaa
    29521 gtatttcaga atacagctgc gcataaggat cagtgctgcg gaggtggatg cctccctgat
    29581 ctggctgcgg gttccggaga aatgatactc tttcaccgta acttctccct ttacgctgca
    29641 tccgatatat accagtccta ccggcttttc ctcggttccg ccgtccggtc ccgcaattcc
    29701 ggtcactgcc acagccacat ccgatttcat taacagtgcg gctccttttg ccatctctga
    29761 ggcagtcttg ctgctgacgg caccgtattt gtccagcgtg ctcttacgca ctcccaggaa
    29821 tttccgtttt gccttattgg aataagtcac cagtccggcc ttgtagcact ccgatacccc
    29881 ggggacattg atgatccttg ccgcgatcat gccgccggta caggattccg cggttgtcac
    29941 cgtcagatca ttggccatca gcagatctgc taccgccatc tccagggtag tctccgcctt
    30001 ggtactgtaa atatccgctc caaaacggga tttcagttcc ttcaccacag gtttaatggc
    30061 ttttccggct gccttggtat cttccccgga agcggtgaca cggatatgta cttctcccgt
    30121 cttggcatag gtagctatcg taggattggt ctgtccgtcg atcagatcct tcagggtatc
    30181 ctccacctgg ctctccggca caccacaaag ctttaccatc tgagaacaga tcaccccggg
    30241 agtcagtgta tccagataag gaagcacctg ctgttcgaac atgggaacca gttccgcagg
    30301 ggggcccggc agcaacacaa tacggttctt ctccgtctcc agaataatgc ccggcgctgt
    30361 tccattatca ttgtccagca ccttcgctcc ctctggcacc atggcctgtt tccagttgtt
    30421 ctccgtgggc tgaatatctc tgactgcgaa aaattcggca atgcgctcca tgctgtgatc
    30481 atccaccgag agattctttc cacagacttc cgccacagtc tccttggtca gatcatcctc
    30541 cgtgggtccc agtccaccag ataacaggat cacatccgaa cgctccatgg cacatttcaa
    30601 cacacctgcc agtctctcca cattatctcc caccactgtc tggtagtaac agtccagtcc
    30661 ggctcccgcc agcttttccg ccagataagc tgcattcgta ttcacaatgt tccccagtaa
    30721 taattcggta cctacacaga tgatctccgc tgtcatatct ttaccgcctt tcgctgtcac
    30781 ttgtcctata tctttcccac agacagtata ctacacttct cgggcggaga aaaggaaata
    30841 ttttactcca gagaattttc actttccact ttttcctgat tccagagctg gtgtcgtatt
    30901 ttttcatccg ttcccttgtg actccgctcc cgccactgag taggggtgca ttcatacctt
    30961 ttctgaaaac agcggtggaa ggtccgcata ttgggaaatc cgcattcgta actgatctct
    31021 gtcaccgtct ttctgctgtc cttcaacaga ttgcaggcca ggatcatccg ctgctgcgct
    31081 acataggaag gaaaagagat atttaactgg gaggaaaaaa tatgggacag ataatacgga
    31141 ctaataccca actcttttgc caccttctgt gccgtcagtt ccccggtgat attgtcgttg
    31201 atatattgca aaagcttctg cgtgatattc agatccgaag gcttgtcctt tttgatcact
    31261 gggatatttt catacatatc cgccaacagt gccatcagaa gtcccttgtc ctgccgaaat
    31321 ggccgcagtt ccttatgcag accaatatct gccagaatgt tcagtaccat ctgtccgtaa
    31381 cggctgagca tgcttttctg aaaacgggga gactccatat cataataggt aagttcttcc
    31441 tgaaattccc cgatggaatc catatctgca atgatcagaa gcattcccac ctctcccaca
    31501 gaactgtagg aatggatctg gttcgggtag atcatcagac agtctcctgt aatcagatga
    31561 taattcacac cagctaccgt ggcatccatc tccccttccg tcacatggac gaattccata
    31621 tgtttatgca gatgaaacgg gaagttcggg tgccaccaga catgacagtc atagaccttt
    31681 ttccggctct cataaaacgg ttttactgtg ttcataccaa tccccatacc tttcacagtc
    31741 tgcaaacttt aggccgcagg cacaaaattt aaagctctct gtattctaca taccatattc
    31801 cgtcccgaat ttctacctgt tttgttaaaa atagcaattt ttgtcccata tgtaatttct
    31861 ttttgtcgtg cagtccacac ttttttcact tacaataagc ttaaatgaaa gcagttccaa
    31921 tttaaaaaac aaaaagtgag atatattatg aatcgtacag aagccagaga aaaagctacc
    31981 gcactggtag cacaaatgac aatagaagaa gctgcttccc agctgttaca ctcgtctccc
    32041 gcgattcccc gtctgggcat tcccgcctat gactggtgga gtgaagccct tcatggtgtt
    32101 gccagagccg gtactgccac ctgttatccg caggcgattg gcctgggtgc aacttttgac
    32161 cgtgaattat tacagaaaat cgccggttcc atcgctttgg aagcccgggc aaaatataac
    32221 gcttattccc gcctcggaga ccgcacccgg tacaaaggtg tgaccatgtg gtctcccaat
    32281 atcaatatct tccgtgatcc ccgctgggga agaggccagg agacctacgg agaagatccc
    32341 gtacttaccg cttcccttgg ctgtgccttt gtggaaggat tacagaccaa aagagacggt
    32401 tacctgacca ctgccgcctg tgccaaacat tttgcggtgc attccggtcc cgaggctctg
    32461 cgtcacagtt tcgacgccaa agctacgaag aaggatctgt gggaaactta tcttcccgct
    32521 tttgaagctc tggtgacaca ggctgatgtg gaagctgtca tgggtgctta taaccgtacc
    32581 aacgaggaac cctgctgtgc tcactcctat cttatggagg aggtgcttcg cgacaaatgg
    32641 cattttgaag gacattacgt ttccgactgc tgggcgatcc gtgatttcca cgagggacat
    32701 catgtaactg catttccgga ggattccgcc gctcttgccc tggaaaaggg ttgtgattta
    32761 aactgcggct gtacctatga atatatttta caggcatata aacagggaaa ggtaaccgaa
    32821 gaagaaatcc gccgcagtgc agaacgtctt ttcaccacca gatacctgtt aggactcttc
    32881 gatcacagtt ccctggacga aattccctac aatgtggtgg gctgtaaaga acatcgggaa
    32941 ctggcttatc aggccgctgt ggaaagctgt gttctgttga aaaatgacgg tacactgcct
    33001 ctgtccttga aagataacaa acgcatggct gtcatcggcc ccaatgccga cagtcatgcc
    33061 gcactggttg gtaactacaa cggtaccccg ccccgttcca tcactgtagt ggaaggaatt
    33121 acaagaatct gcgaggatac cggctgggat gtcaattatg cagagggatg tctgctctat
    33181 gatgaccctt ccgtacgaaa ggtattccag aactaccgta tccccgaagc tgtggcattg
    33241 gcagaatcct gtgacctcgc cattgtatgt gtcggtctcg actccacact ggaaggagaa
    33301 cagggagatg tgggtaatgc atttgccagt ggagacaagc cagatctgct gcttccaaag
    33361 atccagaggg atctggtaga acaggtcatc gctaccggta ctccggtgat tctgattgat
    33421 ctggccggaa gccccatcga tctgtccgct tacgaagatc aggtgcctgc aatactgcat
    33481 gcctggtatc ccggcggaga aggcggacgg gccattgcag acatcctgtt cggcaaagta
    33541 tctcccggcg gcaagctgcc ccttaccttc tattataatg acggaccgct gccagacatt
    33601 accgattatc atatgaccgg acgcacttac cgttattttg aaggcaggcc ctggaagcct
    33661 tttggtttcg gactcaccta cggagaatta cagatcacag aagcaaaagt aactgaagtt
    33721 gattacacga aggagattcc ttccgcacaa atcacaatac attgccagaa tcccacggat
    33781 cgtgacttaa gtgatgtcat acaggtatat gcccatgtaa acggttcttc caacgaagtt
    33841 cccaaccaca agctggctgc gttcgaacgg atccgtctgg atgccggaga aagcaaagaa
    33901 ttccggatca ccattccgcc catggccttt accacggtag acaattccgg caacagagta
    33961 tttgatggca cttctgctac actttatctg ggattttccc agccggactt cacagaagaa
    34021 gatcagaaga tctatacagg caacagaggc caggtattcg cggtcacttt ataatgagca
    34081 ttatggtggt aaaccatttg ctatagagag atgtattttt nnnnnnnnnn nnnnnnnnnn
    34141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34201 nnnnnnnnnn nnnnnnnnnn aaacaaggag gataaatcct ctcctcgcca taggggctcg
    34261 gattttcctt cggaaaacaa ggaggattac aatgaaaaga aacaagatct taagcctgtt
    34321 gctgtccgtt tccatgctga ctgcactcac cgcctgcgga agtcagagtt ccgatactcc
    34381 cgcagcatcc accaccgata acggaagcac tactgctgct accgaaagca ccgcttcttc
    34441 cgatgacggt tatgtactgg acaaggttac cctcgtcgta gacggtacct tcaatgcatc
    34501 tgtggatgct tatcaggaca aattcgtcga acaatgggac acagctgtca gtgaagcact
    34561 gggccatccc atcagtctga atatccagca gttagaccat tccagctatg tagatggcgt
    34621 tggccgtctg tttgccagcg gtgattaccc cgatgtcatt ctgctgaatg ccggtcagta
    34681 tgcagaatat gcaaaaacag gtcttctgtg ggatatgaca gctgcttatg ataatgcaaa
    34741 gttccacagc cacatggtac tgcccgcagt aaacgagaat gtccgtatcg acggcagaca
    34801 gtatggtctg tccaccggtc ttggcggcgg ctgtatcact tatgtaaagc aggcatggct
    34861 agatgcagtc ggcatgaagg ctgaggatat taccgactgg gattcctact atgcaatgtt
    34921 aaaggcattc accgagcagg atcccgatgg caatggcaag aacgatacct acggcgtagc
    34981 tgcagcaggc tttatcggtt ccgaggctcc ttacaccaac tatctgcctc agttctggca
    35041 gaacgcatat cccagcttca cctatgatga gaacggtgta tggtatgacg gtttcaacac
    35101 ccaggagacc aaagatgctc tgcttcgttt acagcaggct tacgcagacg gtgtcatcga
    35161 tcccgaatcc ctgaccatgg gtaccaagga tgtccgtgaa aaatggtggt cttccgatca
    35221 gtccggttcc ttcggtgctt tcacctactg gtccggttac tggaacgata acttggttaa
    35281 caacatggat aagaacggtg tagattccgg actggcaaga ctggccccca tcgctgaaat
    35341 ggatggctac ctgaacagag aatctcctgt atactgcatc attgatgacg gtgacggcga
    35401 tgattccaga gaacaggcaa tcttcgatgc agtcttcgag accatgttcg acggcggtac
    35461 tgtacagacc ctgtgggtat acggtgcaga aggctaccat tggtctactg aggctgagac
    35521 tattgttacc ggtgaaggta ctgacaatgc caagacctat gaatacaaag acggtgagtt
    35581 ccatctgcgt ctgaatcctt ccgatcccag tgcattatgg aaaaagaatg ctatcgatcc
    35641 ttcttccatg atctgctcct tagagaatgg ttttgaatcc gctaccgatc tgaccaagga
    35701 atgtaacgag ttcttctccg aacacagcgt agacgctccc cactctgctt cctgtgacgg
    35761 tattaccaac tacggcggta cgatcaatga tgccaaaaac gtagtcatcg ccgaggttgt
    35821 tgtaaagggc ggagatgtag atgccgctat ggacaattat gttaagacta cccaggatat
    35881 ggtagacgag atcctcggac agctgaatgc agactaaata gtaaaaaata accgattagc
    35941 aacctgctcc gccgggcgca ctaaaaaagc cgcttcacaa aaatgtgaga cggcttttta
    36001 cggtaactgg aa
//
LOCUS       DAWEJW010000015        35183 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_538_length_35183_cov_69.389461, whole genome shotgun sequence.
ACCESSION   DAWEJW010000015 DAWEJW010000000
VERSION     DAWEJW010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 35183)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 35183)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..35183
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_538_length_35183_cov_69.389461"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11028..11127
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    28642..28741
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    30806..30905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9951 a         7403 c         9511 g         8018 t
ORIGIN      
        1 taaggcaacg gactctgact ccgtcatttc aaggttcgaa tccttgtagc cctgcgaagc
       61 aggggttgac ctgctaatgc aaacctcagt agagaaatct gctgaggttt tttgttgtcc
      121 ggaaatatct cagatattca tggtgaaatt cccctgattg tgaataaaag ttggcaataa
      181 tacacaaaaa agccacacaa agttgaagag aattataaaa tgtgataaga taaacataaa
      241 attaaggtag gagatctgca aaaacggaca aacagaaaag gggaatctta tgaacaatat
      301 aaaagtacag acgaaaatga tcatagtaat actggcaacg atggctgcac tgttgttgtg
      361 tactatcttt tcagcacaaa gcatgaaaga attgcagtcc aaagcattag ataccatgga
      421 gactgatgag cgggaagcct acgatgaaca gatcaagcag caggtagata atgtgatctc
      481 gctctgccag accatttatg atcagtatca ggctggtgtc tataccgaag cagaggccag
      541 gaaacttgcg gcggatgaaa tccgccagct tcgctacgga gattcgggat atttctgggt
      601 ggatcagtat gacggaacca atgtggtact gctgggaagt gcaacagaag gtacaaaccg
      661 tatggagacc aaggatgcta acgggtacca gatggtaaag gaaatcatcc gcatgggaca
      721 ggagccggat ggtggttatg tagattatgt gttcccaaag gaaggagaga cggaatcctc
      781 tcccaaacgt tcctacagca aagcgtttga accgttccag tgggtcatcg gcacaggaaa
      841 ttacaccgac tatattgatg ataagattgt cacggtaggt gatgaatttt ccggttatgt
      901 gacaggaaga ctgacgatgt tcattgcatc gacacttata gaaggtgcaa tcgtgatcgt
      961 ccttctggtt ttcattatta tcagcatcgt ccagccgctc aaaaaatgtg tggcatccat
     1021 cggcatgatg gaacagggag atttctcaaa atctatggga gataagcttt taaaacgccg
     1081 tgatgatttc ggacggctgg cagtctctct ggagtctatg aggacagaga tgagtggact
     1141 gatcggtgaa gtcaaagagc agactgcaga gatcaacaat atggtacaga atattgataa
     1201 cagtattcag gctctggatg aacagatcga ggatgtgtcc gctaccacag aggaactggc
     1261 agccggaatg gaagagacag cggcatcttc tgaagagatc aatgctatgt ctcatgagat
     1321 tgagagtgca gcgaagaata ttgcgacacg ttctcaggat ggtgccaccg aagcagatgc
     1381 gatccgtgag cgtgcggtag gaatcaagaa ggatattaca caaaatgatc agcgtaccaa
     1441 agccattcat gaagagatca acgaaggact gaccaaggca ctggaggaaa ttaaagtagt
     1501 caaccagatt ggagttctgg cagaatctat tatgcagatc acgggacaga cgaatctgtt
     1561 ggcactgaat gcatccattg aggcagcacg tgcgggagaa gccggaaaag gatttgctgt
     1621 ggtggcggag gaaattcgtg tgctggcaga acagtccaag gcagccgtct ctcatatcca
     1681 ggatgtgacc aacaatgtga tggatgctgt aaacaatctg gcagaaggaa cacagaagct
     1741 gttgggattt gtcggaacag atgtggtgga cagctttgca gcattttccg gtatggcgga
     1801 ttcttacagt aatgatgccg gtcagatcga tggactggta acggatttct ctgcctcctc
     1861 cgaagaactg ctggcatcta tcagcggtgt catggaggca atcaacgaag tcagcaaggc
     1921 agctaccgaa ggggcttcgg gaactacgga tattgcggat aagacgggga taatcgtaga
     1981 aagagccaac gagatcaagg aaaaagcgga ggctgcaaga attgctgcgg acaaacttca
     2041 gcaaaatgtg gaacacttta tcgtgtagag aatgtgcatc atgcacagga agaaaaatat
     2101 aaaattgtgc aaaaagcact tgaaaagtaa aaagttaagt gataatataa gccacaacaa
     2161 gaaataaacg aaaaatatta tcattcatgc aaagaagcat ttgtagtgat acgaatgctt
     2221 tttttgcgtt atctgcagca ggcggatcag gcggtgagaa acggcggtac cagcgcagga
     2281 ctgtgtggca gagagatccg gggcaaaaag gtgggaatca tcggctgcgg tcagatcggt
     2341 ttcatgacag cgaagctttt ccgggcattc ggtgcggaag tctatgccta tgccagacac
     2401 gaaagagaag aagtgaaggc agcaggaatt gcatacaaac cgctggatga cctgttgaga
     2461 gagtgtgata ttatttccct gcatacgccg aacaacaagg agaccagagg aatgatcagt
     2521 gcggagaaga tcgcactgat gaagaaatca gccattttta tcaactgtgc aagaggcctt
     2581 atcgtagaca gcaaggcact ggcacaggct ttgaacgaag gacggatcgc aggagctgcg
     2641 gtggatgtat ttgactgtga gccgcccatt cccgcagagg aaccgctgct tcatgcgaaa
     2701 aatacattgt tgacaccgca cgtggcattc ctgtcggagg aagccatggt acgcagagcg
     2761 gaaatcgagt tttccaatgt gtacgcttat ttggacggaa agccggaaaa cgtctgtgcg
     2821 ctatgatgcg gagcggaaac agcaaaccga acatattttc gaattcatct gtacttttca
     2881 aaaaaattct gctataatct gtgcgtaacg tgaaaaaata gataacagtt cagccgtgaa
     2941 cgtatccggg aacaggatca tgcctgtatg attttagaac cgaatacgtt cacgaactgt
     3001 gttattccag taacatattt gcacggagag gcatcatgaa tccatcgaac gaaccctcac
     3061 aattcaaatt acattccgaa tatcagccta ccggagacca gccgcaggcc attgcggagc
     3121 tggtggaagg cttccggcag ggtaatcagt ttgagacatt gctgggtgtt accggttccg
     3181 gtaagacctt taccatggcc aatgtgatcc aggcattgaa taagccgacg ctgatcattg
     3241 cccacaacaa gacactggcg gggcagcttt acggggaaat gaaggaattt ttcccggaaa
     3301 atgccgtgga atattttgtc tcctattacg attattatca gccggaggct tacgtgccgt
     3361 cttcggacac ttatattgcc aaggattctt ccatcaatga tgagatcgat aaactgcgtc
     3421 tgtcagcgac agcatccctg gcggaacgca gggatgtggt ggtagtggcc agcgtgtcct
     3481 gtatctatgg tctgggaagc cccgacgaat atcaggatct gatcgtatcc ctgcgtcccg
     3541 gcatggtgaa ggatcgtgac gaggtattgc gggaactgat cgatattcag tacaaccgca
     3601 acgatatgga tattcagcgt gccactttcc gtgtcagggg agatgtggtg gaagtatatc
     3661 cggcacaggg cggcgattat ctcatccgta tcgaattttt cggggatgag atcgaccgga
     3721 tcgcagaggt agagcctctt acagggaaaa ttcatgcaga gctgaatcat gtggcgatct
     3781 tcccggcatc ccattatgtg gtatctcagg agaagatcaa ggcagcctgt gacaatatcg
     3841 aagaagagat gaaggaacgg gtacagtttt tcaagggcga agacaagctg atcgaggccc
     3901 agcggattgc ggaacggacg aatttcgata ttgagatgct ccgggagacc ggtttctgtt
     3961 ccggtatcga gaactattcc agacatctga acggacttcc tgcgggagca ccgcccatga
     4021 cgctgttgga cttttttccg gatgattttc tgatcatcgt ggacgagtcc catatgacga
     4081 tcccacagat ccgcggtatg tatgccgggg accgctcgcg aaagcagaca ctggtggact
     4141 acggtttccg tctgccctcg gcactggata atagaccgtt gaactttgag gaattcgagg
     4201 cgcatatcga ccagatgatg ttcgtatccg ccactccttc cgattatgag aaggagcatg
     4261 aactgctgcg gacagagcag atcattcgtc ccacaggtct gctggatccg gaagtacagg
     4321 tgcgcccggt agagggacag atcgatgacc tgatcggaga ggtcaacaag gagatcgcac
     4381 agcatcataa ggtactgatc accacactga ccaagcggat ggcagaggat cttacggatt
     4441 atatgaagga agtcggtatt cgggtgaaat atctgcattc tgacattgat acgctggagc
     4501 gtgcccaaat catccgggat atgcgtctgg atgtgttcga tgtactggta ggtatcaacc
     4561 tgttgcgaga gggtctggat attccggaga tcactctggt ggccattctg gatgcggata
     4621 aggaaggatt ccttagaagt gcgacttccc tgatccagac catcggtcgt gcggcccgta
     4681 atgcggaagg ccatgtcatc atgtatgcgg acaccatcac ggactccatg cgggaagcac
     4741 tggatgagac caacagacgt cgtgtgatcc agatgaaata taatgaagaa catggtatca
     4801 ccccccagac tatcaaaaag gcggtgcgtg atcttatcag catctccaag gtaatcgcca
     4861 aggaagaggt acgctttgag aaggatcccg aatccatgac gaagaaggaa ctggagaagc
     4921 tgatcggcga gatccagaag aagatgcaga aagcagcggc agacctgaat ttcgaagcgg
     4981 cagcagaact tagagataaa atgcttgaat taaagaaaca attaaatgat atggaataaa
     5041 agtaaagcat caaagatgcg attttgcgaa tgggcctctg aatagttgga gaacgatatt
     5101 ttatttaaga aaggcaagat aatggaagaa gcaaagaaga tgcgtccccg ggagtacatt
     5161 aagatccggg gtgccaatga acataacctg aaaaacatta atgtggacat tcccagaaac
     5221 gagctggtgg tattgaccgg tatgtccggt tccggtaagt cctctctggc attcgacacg
     5281 atctatgcag agggacagag acgctacatg gaatccctgt cctcctatgc gagacagttc
     5341 ctgggccaga tggagaagcc caatgtggaa agcatcgaag gactgtcccc cgctatctcc
     5401 attgaccaga aatccaccaa ccgtaaccct cgttctaccg taggtacggt aacagagatt
     5461 tatgactatt tccgtctgct gtatgcgaga atcggtattc cccactgccc gaagtgtggc
     5521 agagagattg ccaagcagac tgtagatcag atggtggatc agatcatgaa catgggagaa
     5581 ggcacgaaga tccagttgct ggcccccgtg gtcagaggcc gcaagggcga gcatgccaag
     5641 gtgttggagc gcgccaagag aagcggttat gtccgggtac ggatcgacgg cagcatgtat
     5701 gaactgacgg aagagatcaa actggataaa aatatcaagc acaatatcga catcgtggta
     5761 gatcgtctgg tggtaaagga tggtattcaa cgccgtctga cagactctat tgaaaacgta
     5821 ctggaactgg cagaggggct gctggtggtg gatgtgatcg gcggcgaacc ggtgactttc
     5881 agccagagct tttcctgtcc ggactgtggt atcagtgtca gcgaagtgga gcccagaagc
     5941 ttttccttca acaatccttt cggtgcctgc ccggtatgct tcggcctggg atataagatg
     6001 gaattcgacg aggatctgat gatcccggac aagagactca gcatcaatga gggagccatc
     6061 acggtcatgg gctggcagtc ctgtgcggac aagtccagct ttaccaatgc tattctgcgg
     6121 gcactggcga aggaatacaa ttttgatctg gatactccct tccaggatta tccccagaag
     6181 attcatgaca ttctgatcca cggcaccaac ggtaaagagg tcctggtgca ttataccggc
     6241 cagcgcggca gtggtgtgta tccagtagcc tttgaaggcc tgatcaaaaa tgtggagcgc
     6301 cgctatcgtg agaccgcttc cgagagcagc aaacaggaat atgagacttt catgcgcatc
     6361 actccctgta aagaatgtaa gggcatgcga ctgaagaagg aatctctggc ggttaccgtg
     6421 tgcgacaaga atatttatga gatcacatcc atgtccatcc gtgatctgca gaaattcctg
     6481 gataccatga cgctgacgaa acagcagcag ttcatcggtg agcgtgtgct gaaggagatc
     6541 cgcgcaagag taggatttct ggtggatgtg ggactggaat atttgtctct ggccagagct
     6601 acggctaccc tgtccggcgg tgaagcacag cgtatccgtc tggctaccca gatcggttcc
     6661 ggactggtgg gtgtatgcta tattctggac gagcccagca tcggcctgca ccagagagac
     6721 aatgacaaac tgctgaatac tctgaagaac ctgcgggatc tggggaacac tctggtagtg
     6781 gtagagcatg atgaagacac tatgctggca gcggattata tcgtggacat tggtccggga
     6841 gccggttccc acggcggaga ggtcattgcc tgcggcacag cggaagagat catgcagatt
     6901 ccggaatcca ttacgggaca gtacctcagc ggcaaaaagc ggattcccgt accggagacc
     6961 agaagaactc ccaccggctg gatcaaagta ctgggagctc aggaaaacaa cctgaaaaac
     7021 gtagatgtag aaattcctct gggtgtgatg acctgtgtca ccggtgtctc cggttccgga
     7081 aaaagttcac tggtgaatga gatcctctac aagcatctgg cgagagaatt gaaccgtgcc
     7141 cgctgcattc ccggcagaca caagggtatc gaaggtgtgg aacagctgga caaagtcatc
     7201 aacattgacc agtctcccat cggacggact cccagatcca accccgctac ttataccgga
     7261 gtatttgatc tgatccgcga tctgtttgct tctacggcgg acgcaaaggc caagggttac
     7321 agcaagggac gattcagctt caatgtcaag ggcggaagat gtgaagcctg cggtggcgat
     7381 ggtatcatca agatcgagat gcatttcctg ccggatgtct atgtgccctg tgaggtctgc
     7441 ggcggtaaga gatacaaccg tgagacactg gatgtaaaat acaaaggcaa gagtattttt
     7501 gatgtgttgg atatgacggt ggaagaagca ttgcctttct tcgaaaatgt accgtttatc
     7561 cgcagaaaga tcgagaccct gtatgatgtg ggactttcct atgtaaagct gggacagcct
     7621 tccactaccc tgtccggtgg tgaggcacag cgtatcaagc tggccacgga gctttccaga
     7681 agaagtaccg gcaggaccat ctatatcctg gatgagccta ccacaggact gcattttgag
     7741 gatgtccata agctggtgga gatcctgcac agactggcgg acggcggcaa cacggtggtt
     7801 gtcatcgagc ataatctgga tgtgatcaag accgcggatt atatcatcga tatgggaccg
     7861 gaaggcggag accgcggtgg tacagtgatc gccaagggaa ccccggaaga aatcgtcaag
     7921 gtgaaaaaat cctacaccgg ttactatgtg aaaaagatgc tggagaagga caagaaacta
     7981 cgataactat tcagaccccc attcgcaaaa cgctctttca tggctccctg attctgaata
     8041 gttatcaaat tttaagaatt accctctatg aaaatggaaa aaattcagtt ataatatgat
     8101 gtatatgact aaaatagcgt gtagagtcaa gacttataga tagataccat cggaaagggg
     8161 taataaaaaa gatgcagtgc acagatatcg gaattgattt aggtaccgcc agcattctgg
     8221 tatacatcag aggaaagggc gttgtattaa aggagccctc tgttgtagct tttgatagag
     8281 atacaaataa gattaaagcc atcggtgagg atgcaagact gatgctggga agaactcccg
     8341 gcaacatcgt agccgtaaga ccgttacgtc agggcgttat ctctgattat accgtaaccg
     8401 agaaaatgat gaaatacttt atccagaaag cgctggggaa gagaactttc cgtaagcctc
     8461 gtatcgctgt ctgtgttccc agcggcgtta cagaggtaga gaagaaggca gtagaagatg
     8521 ctacctacca ggcaggtgcc agagaggttt ccattattga ggaacccatc gcagctgcta
     8581 tcggtgcggg tattgatatt tccaagcctt gcggtaatat gatcgtagat atcggcggcg
     8641 gtacttccga tatcgcagtc atatctctgg gaggtactgt agtcagtgct tccattaaga
     8701 tcgcaggcga tgattttgat gaagccattg tgcgttatat gcgtaaaaaa cataatctgc
     8761 tcatcggtga gagaaccgca gaggatctga agatcaagat cggttcctgc tacaagagag
     8821 cggaagtgga ttatatggat gtcagaggac gtaacctcgt gaccggtctg cccaagactg
     8881 ttaaggtgtc ttccgaggag accgaggagg cattgcgcga gactaccgca cagatcgtgg
     8941 agaccattca cagcgtactg gagaagactc cccctgagct ggcagccgat attgcagaca
     9001 gaggaatcgt actgaccgga ggtggaagcc tgctgcgcgg tctggaagat ctgatctctg
     9061 ccaagacagg tatcaacacc atgacagcag aggatcccat gacagcagtg gccattggaa
     9121 ccggtaaata cgtagagttc ctggcaggct acagagagta acacagacag ctccggggaa
     9181 ccggaaaaga tagactaagg caggatgccg tacatttgtc gaaggatcgc agccgcaaat
     9241 ggaggatatg aatggttaaa ggcttgtata ccgcttacac cggtatggta aacgaacaga
     9301 acagaatgga tatcatgacc aacaatctgg ccaatgcatc taccgtcgga tataaaaagg
     9361 aaggatccac cagccagtcc tttaatgatg tgctgacggt caagatcaag gaccagtcgg
     9421 taggcatgag aaatgtccag aagatcggta tcaaaaatcc cggagtgaag atcggagaga
     9481 attataccga ttatacgcag ggatctttcc gcattacgga taatacctat gatctggcat
     9541 tagccggaga tggctttttt gccattgaat ttaccaataa ggcaggagag accgacacca
     9601 aatacacccg tgcaggacag tttacactga ataaggatgg atatctggta acggaagagg
     9661 gtgactatgt tctggatacc cagaacagga ggatccgcct gaacacactg ctagattcca
     9721 agatcaccga tgacggtacg atttatcaga acgatcaggc agttgccaga attcaggtga
     9781 cggactttga agattatgat tacctggaaa aatatggaga gacctattat cagcccattg
     9841 aaggggccaa aacgactacg gcagatgcac aggtgaagtc aggctatctg gagatggcaa
     9901 atgtacagat tgtatccgag atggtaaatc tgatctccat taccagagca tatgaatcca
     9961 accagaaggt ggtccagacc attgatggca ctctggatgt agcagcgaac cagattggta
    10021 aactgtagta atgaattcgg ctgggacagc cgataactga ttatagaata ttttcaggat
    10081 aaaaatcagg tcatggatga cataagggac attgtgagag gagactacat atggtcagaa
    10141 gcttatggac agcagcgacc ggcatgatcg cacagcagac aaatgtggat acgattgcca
    10201 acaatctggc aaacgtaaac accacaggtt acaaaacaca ggtgaatgaa ttcaagagcc
    10261 tgttatacca gaacatccag acgaagacaa cttccgcaaa cggagatacc aagcccagca
    10321 gtgcgcaggt aggtctcggt gtaagaaatg cttccatcag ttccgtattt aaggaaggaa
    10381 gtctgacagc atccgacagc gacaccgcat ttgcaattga tggcaagggt ttctttgcag
    10441 taagaggagc ggacggcaat acctactaca ccagaaacgg taatctgaag tttactcttg
    10501 cagccaacgg cggcaatatg ctggctactt ctgatggact tccggtactg gatacacagg
    10561 gcagacccat tgtactgggc agtaattatg tgatgagcaa tgttacggtg agcaaggacg
    10621 gaagtctgtg ttatcccgat gcaaagaaca atccccagcc tatcggcatc acaataggtg
    10681 ttttccagtt caataatccg aacggactgg aaagactggc agacagcctg tatcagcaga
    10741 ccgcagccag cggacaggct atcaacgaag caagaaataa taatgtagaa aagagcagta
    10801 ttatccaggg atatctggaa agctccaatg tacaggtcgt agacgagatg gtcaacatga
    10861 ttgttgcaca gcgtgcctac gaactcaact ccaaagccat cacagcatcc gatgagatgc
    10921 tacagcaggc caacaacctg cgtaggtaat gagacatgtc aaaccgtaag atgacgattc
    10981 gcctgcttgc aggcggaaac gtcagattgc ggtttgatag ggcgaatnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnccc gacatcgaag gaaacgcgaa gcgtttacga
    11161 gatgcattgc tcattaccgt agggacgatt cgcctgcttg caggcggaaa cgtcagattg
    11221 cggtttgata gggcgaatgc ccgacatcga aggaaacgcg aagcgtttac gagatgcatt
    11281 gctcattacc gtagagcata gaaaacctaa aaactaagga ggcccattta tggatatctc
    11341 ttctatatac ggcagttccg ctctggacag caccgcatac accaatgcgg cgaaccagac
    11401 ggcttccaaa ttgcagaacc agttaaacgg ttcggattac accaaagcaa cagacgatga
    11461 actgatggat gcctgcaagc agttcgaggc ttatttcctg gagcagatgt tcaaggaaat
    11521 gatgaagacc atccctgaga gcgatgagac ttcttcttcc aactcccagc ttatggatta
    11581 ctataaggat gagatggtac agcagatcgc atccgactct acggagcaga acagtctggg
    11641 actggcacag atgctatatg agcagatgaa gcgtaattac aatcttgagt agcgacttcg
    11701 ggctgccatt tctggcagcc ctgatctttt ttatcttacg ccgccaccgc ggtgacctct
    11761 tcccaattcg gagagaacag ccttgaaacc cacaaatcaa cagacagata gtatgaggaa
    11821 taaaaatgga gacgattaca ggttttgtag accatattgt gtttcaaaac agtgaaaacg
    11881 gatatacggt aatgatcctg tcgacagaag gggaagagat gacctgtgtc ggtatgtgca
    11941 aaggactgac ccagggagaa aatatttccg cagagggaga atacatagaa catcccgtat
    12001 acggccgaca gttcaagatc cagagctacg agaccgtaac accgaccgac cgcgtgggaa
    12061 tggaacggta tctgggttcc ggagcaatcc ggggagtcgg tgaggcactg gcagccagaa
    12121 tcgtaaaaaa attcggagac gatactttcc gcatcatcga ggaggagccg gagcgtctgg
    12181 ctgaggtaaa ggggatcagc gaacgcaagg cacaggagat cgctatccag atggaagaaa
    12241 aaaaggatct ccgggaagca ctggtctatt tacagcaata cggaatcagt aatactcttg
    12301 ccgtaaaaat atataatacc tatggtatgg aaatgtacag cgttatgcgg gagaatccct
    12361 atcgcctggc agaggatgtc agtggtgtgg gattccgtat cgcggacgag atcgcaggga
    12421 agatcggcat tcataccgac tcggattacc gcatccgcag tggcattctg tataccctgc
    12481 tgcaggcggt gggagaaggt cattgttatc tgccgttgga tcttctgctg cgcagagctt
    12541 ccgaactatt gggggtatca gaggaaaata tccgtcctca ggtggataac cttatcatgg
    12601 accgaaagct tgtggcaaag ggagaagcag tatttgcttc ctcttattac tatgcggagt
    12661 tgaattgtgc caatatgctc aggaatctga atatccctat ggtggaggca gaaaatcttc
    12721 cctcccagga tgcagcgatc cgcaaacgtc tggaacgcat ggcggaaaat ctgtccatgg
    12781 aactggatga actgcagctg aaagcggtgg aggaaagtat caaaaatggc ctgtttatcc
    12841 tgtccggagg tcccggcacc ggaaaaacca ccaccattaa tatgattatc cgattctttg
    12901 aatcggaagg catggacatt tttctggcag cacctacggg cagagcagcc aagagaatga
    12961 cggaagccac cggatttgag gcgaagacca ttcatcgtct gttggaactg aacagtgccc
    13021 tgtcggaaga cgacaccaga aaggcaaatt tcgagcggaa ccaggagaac cccttagagg
    13081 cggatgtggt gatcattgat gaaatgtcca tggtggatat tcaactgttt caggcgcttc
    13141 tgaaagcaat tcttccggga acacgactga tcctggtggg agatgtggat cagctgccca
    13201 gtgtgggacc cggacaggta ttgcgggacc tcatgaacag ccgggcgttc cccatggtaa
    13261 ctctggagaa gatcttccgg caggccggac aaagtgacat tgtcgtcaat gcccatcgga
    13321 ttaacagagg agagcagatc actctggaca ataagtcaaa ggatttcttt ttactggaaa
    13381 gaaatgacgt aaatgtcata tataagcaca tgatccagct gattcaggat atgctgccca
    13441 gatatgtggg agccacctcc tttgatattc aggtactgac ccctatgagg aagggaagtc
    13501 tgggctgtga gacgttgaat gagatcttac agcgttatct gaatccggcg gatcctcata
    13561 aaaaggagca tgcctatggc aataccgttt ttcgggaagg cgataaggtc atgcagatca
    13621 aaaacaatta ccagatcgaa tgggagatcg tcgggagata caatattccc atcgacaagg
    13681 gaatgggcgt attcaacgga gatatgggaa gggttaagga aatcaatgat accatgtcca
    13741 cactgctggt ggaattcgat gacggaagac gggtcaacta tcctttctcg gcattggagg
    13801 aactggaact ctcttatgcc attacgatcc ataagtccca gggaagcgaa tatcctgcgg
    13861 tgatcctgcc actgttgggc ggccccagaa tgctgttcaa ccgcaacctt ctgtacaccg
    13921 gaattacccg tgccaggaac tgtgtcacca ttctgggaag cagtgagacc gtgcggaaca
    13981 tgatcgagaa tgtcagcgag aatcgcagat atacgggatt ggagcagcgg atccgggaga
    14041 tctgctgtct gccggaaaat gatacaccgt agacggaaaa caaaacggga gcctgagatt
    14101 gcagaaagag atcagaaaaa ccataagcaa aataaaggaa acgggattac agctgctgtt
    14161 tcccggaaga tgcccggtct gtgacgggat cgtccgtcct tttggcagga aaatctgccc
    14221 ggaatgtctg cctaaattaa agccggtgac ggctccgtgg tgtatgcgtt gcggaaagaa
    14281 actggcggag gaacgggagt attgcggtga ttgtatgcac cgggaacata aatacgacag
    14341 agccagaact ttatacgaat acagggatgt ggctccgtca atctatcgtt ttaagtatag
    14401 tggacggcag gaatacggcg attttttcgg agaagaaatg gcgcgtctgt tgggagattt
    14461 tatcggccgg gtgcgtccgg acgttatagt tccggtgccg ctgtaccgtg ggaaactgcg
    14521 gaaaagagga tataaccagg cagcatgtct ggcaagggct ttgggcagaa gcctggaact
    14581 gccggtggat gagaaactgg taaaacgggt gagaaataca gcaccgatga agcatttaaa
    14641 tcccacagaa cggcaaaata atttgaaaaa ggcttttatt ataggcagaa atgatgtaaa
    14701 attatatgac cggattattc tggtggatga tatttataca acaggtacga ccctggatga
    14761 gatcgcagca ctgttaaaag agcacggagt gtcaaaagtg tattgcgtga cattggcctg
    14821 tgggtctggt gtatgatcag gaaggtgtga gatactatga atataagaaa ttgcagaatg
    14881 tgcggacata tttttaacta tgtggcagga ccgtttctgt gcccgcattg ccgtgagaag
    14941 atggaagcca agttccagga ggtaaaggaa tacatccgta ataaccccgg tgtaagtatt
    15001 caggatgtat cggagaactg tgacgtggag ccgtcccaga tccagcagtg gctgagagaa
    15061 gagagactgg aactgacaga aggatccagt attctgctga actgtgagaa atgcggagca
    15121 ccgatccgca gcggaagatt ttgcgaaaag tgcaaatatg atatggccat ggatctgcag
    15181 agtgtactgc cgaagaagca gactgcgccc cagcagagcc ccagaacatc cagagacgga
    15241 gaaagaatgc gttatctgta agcatttgta agcggaggaa ttatgctgaa cgaagaaaaa
    15301 gtcatactga tgacgcaaat ggcatcttat gaagagaatg aaggcaaaaa gaatatggcc
    15361 atcggccgtt atttccgcag tgactacatc gccatacagg tgttgaagtc cattctgtgc
    15421 gcgacgatcg cgtatggagt ctgctttgcg ttgtttgtgt tctatgattt tgagaatttc
    15481 atgcaggata tttacaagat ggacctgatc ggttttggaa aaaatgttct gctgtactat
    15541 ggaataacgg ttgtagtgta tggagtggca tcttatctga tctgctctct gcgctatgca
    15601 cgggcgaaaa agagcctgaa atgctattat aacaatctga agaagttgaa ttccatttac
    15661 aatgagtaaa cggcctacac agattgacct ggaggaaata tgactgcttt actggaatta
    15721 agagagaatc tcaagaagat ctacagcaga aatgaagctt ttattctgcc ggtgatcaag
    15781 tttttactca gctttatcgt gctgagcatc ataaacggca aaatgggata tatgacaaag
    15841 ctggataaca tggccatcgt gctgatcgta tcgttgctgt gttctttcct gcccaccgga
    15901 tttatggctt tttttgcaat gatgtttgca gtgcttcata tgtatgcact ctctattgaa
    15961 accgcggcgg ttgggctggt tgttttcctg ctgctgtatc tgctgtttct cagatttacc
    16021 gcgaaggaag cactggtagt ggtactgacc ccggtgcttt gtatgctgaa gctgccctat
    16081 gtgatgcctg tggcaatggg actgatcgga accccggcgt cctgcgtatc tgtaggctgc
    16141 ggtgttgtgg tgtattatct gctccagacg gtgattacca atgctccgac catcaactcc
    16201 atgggagcag aggaagccac tgccaaactg cgcctgctga ttgacggaat gctggggaac
    16261 aaggcaatgc tggtgacgat cgcagcattt gccattaccg tgatcgtggt gtatctgatc
    16321 cggagaatga gtgtggatca ctcctggacc attgccatgg ttgccggcgt tatgatcgaa
    16381 gtgatgatcc tgctggtggg agatctgatg tacgatacca atctctccat cgtcagcgca
    16441 ctgttaggtg ccgttgttac gctgatcgcc tgcaagatca tagaattttt ccgattctgt
    16501 ctggactata gcagaacgga gaaagtacag tttgaagatg acgagtatta ttactatgta
    16561 aaggcagtgc ccaaaatgac agttgcggcg cccaccaata cagtgaagaa aatcaataca
    16621 cagcgacgtc cggcaggtca gcagaccaga acctccggac agggtacgag atctgcaggc
    16681 cagacctaca gaagtacggc acataccggc aggccctcag aaggaacagg ccgaagtgtt
    16741 gtgacagaac gtacccccgc cagaaacgga gttccttacg gacagcaggg cggttaccgc
    16801 ggacacgaga tgtccggtgg cagaagtgtc accataggcg gcaatcacac gaatcctcag
    16861 gatgactccg atgattatga agaattgttc tgaagggatt aatggaaaat gcaattaggg
    16921 gataagctgg cagaatatat taaaaatttc agcagttacc gcacaaccat ggatttcggc
    16981 gatgtggccg agatcctgat cattgcggta ctactgtatt atgcgttagt gtggatgaag
    17041 acgacccggg catgggtatt gctcaagggt ctggttgtca tcctcatttt tttgctgttg
    17101 gcttattttt tccgtatgac taccatcctg tggatggccc agaatgttct gggatttgcg
    17161 gtgacagccc tcatagtggt gctacagccg gaattgagaa aggcattgga ggaactgggc
    17221 aagaagaata ttatcagttc tgtattacct tttgacaaca atcatcgtgt ggacgaggga
    17281 ttttccgaga agaccattaa tgagatcacc aaagcctgtg tggagatggg caaggtcaag
    17341 accggtgccc tgatcgttat cgaacagaag gtttccctgc gggattatga gagaacggga
    17401 atcgatgtgg atggtatcgt gaccagccag ctgctgatca atattttcga gcacaacacc
    17461 ccgctgcatg acggtgctgt gatcgtacag gggaaccggg tagcctctgc cacctgttat
    17521 ctgccgctgt cggataacct gggactgagc aaggaactgg gtaccagaca tcgtgccggt
    17581 gtgggtatct cggaggtgac agattccctg acggtgatcg tatccgaaga gacgggtaag
    17641 atcagcgttg catacgaagg aaagctggag cggaatctgg atgcggagac tcttcgcgac
    17701 agaatgcata agatcctgaa caataccgga gaggaacaca aaaatctgcg aatctggaag
    17761 ggaaggagcc gtgagaagaa atgaaaaaat tactgacacg taatcttgga ctgaagcttg
    17821 cttctctggt gctggccttt gtattgtggt ttctggtggc acagatctat gatccgaagg
    17881 acacggtaac cttcaataat atccaggtca gactgattaa cacagatctg ctggagcaag
    17941 aaggaaaagt atatgaggtg ctggacaaca gtaatctggt aagagtaacg gtaacaggcc
    18001 cccagagtat tgtaaaatcc gaattgcgca gaaacgatat tgtagcggaa gcggatatga
    18061 gcaaactgac agatatcaat acgatcgcca tcacttatta ttgtgagaat atcagcaacg
    18121 attccgtgga gatcaggggt aatcacgatt ccgtgcgcct gaatgtggaa gacaagacca
    18181 gcaaatggat caaactggag agcaccacgc tgggagaggt ggcatccggt tatatgatcg
    18241 gcaatgtaac gttggatcag accaacatcg aagtaaccgg acctaaatca gcgatctcac
    18301 agatcgatca tgccggagtg gacatcaatg tagcagattc caccagcagt ctgtcggcta
    18361 atgtggacat taagctgtat gatgcggacg acaatgaact atctctggaa actgtcaaga
    18421 aaaacgtgga ttctgcgcat atgacagtag aagtgctggc aacgaaggaa gtaccggtag
    18481 agattgaata tatgggtgta cctgaggacg gttatatggc taccggtgaa gtagagagta
    18541 gcaggtctac agtgaagatc gccggaacgg catcagcact gttgggagta tctgccatta
    18601 ccgtaccgga ggatcgtatg aatattaccg gacagtccag cgatctggtg gatatcatta
    18661 atctgaagga gtacctgccc gctaacgtca gactggcgga taagagcttt gacggcaaga
    18721 tcactgcaac ggtatacatt gaaccgatcg cgaccaagga cctgtccgta ccggcagaga
    18781 acattacgat cagcggtgtg ccggagggaa tggaggcaga gatcactaca gaggcggaga
    18841 cttatgatat cacggtatcc ggcctggaca gagatatcag tattctgcgg gatgatacgg
    18901 tgacgggagt actggatctg gagaaatgga tggatgacaa caatgtagaa gaactgacac
    18961 cgggaaatta cacgatccct gtgacgttta acctgtcgga ggatatcatg gtagaaaatg
    19021 atgtcaatat ccacatccga ctgaagaatc aggatacaaa taattaaaag atagaatgag
    19081 aggtttttta tcatggctag aatgtttgga acagatggtg tacgaggaat tgccaacgag
    19141 gaactgactc cgttgcttgc catgcaactg gggcaggcag gtgcctatgt acttacgaag
    19201 gaaaatgagc acaaacccac gatcatggtg ggttgtgata ccagaatttc cggagatatg
    19261 ctggcgaatg cgttgatggc aggagcctgc tccgtaggag ccaactgtgt ctacgtaggt
    19321 gtgattccca ctcctgcggt tgcttatctg accaagaaat acaaagtaga tgccggcgtt
    19381 gtgatctccg catcccacaa tccggtagag ttcaatggta ttaaattctt tgacggtaac
    19441 ggctataagc ttcccgatgc catggaagat gagatcgagg cattgatccg taacaatatg
    19501 cccagtatca aatttcccat cggtcccgga gtcggcaaga tcaagtaccg taccgatgcc
    19561 agagaagagt acatcaatca tgccatgaga gcggtcaatg tggatctgac cggactgaag
    19621 atcgtggtgg attgtgcaga gggtgcctct ttctatacat ccgtagaatg cctgaaagaa
    19681 ctgggcggca gcgtggtggc cattcacaac aatccggacg gtaccaatat caatgccaac
    19741 tgtggttcca cccatatgga agaactgcag gccagagtgg tctatgaaaa ggccaatgtc
    19801 ggtctggcct tcgatggaga cgcagacaga ctgctggctg tggatgagaa cggtaatatc
    19861 gtcgatggtg accagatcat ggcaatcgta ggcaactaca tgaagagcaa gggtactctg
    19921 aagaaggata ccatcgtggc aaccgttatg agtaatctgg gattcttcct gatggcagag
    19981 aagaatgcca tccatatgga gaagaccaag gtaggtgacc gctatgtact ggagcgcatg
    20041 aaggagatcg gcgccagcct cggcggcgaa cagtccggtc acattatttt cctggatgag
    20101 aataccaccg gagacggact actcagtgca ctgcatctgc tgcaggtcat ggtggatacc
    20161 ggaaagcctc tgtcagaatt ggctaaggtg atggaagtac tgccccaggc attggtaaat
    20221 gccaaagtgg ccaatcacaa aaaagagaaa tatatggaat atccggagat cgcagcagcc
    20281 attgggaaac tggaagaaaa attcgccgga gagggccgtg tcctgatccg tccttccgga
    20341 accgaaccgc tggtaagagt catgatcgaa ggaaaggatc agcaggtgat ccaggaagaa
    20401 gcacggaagc ttgccaacct gatacaggat atcatgttct agcagacaga ttccgaaagt
    20461 tgtctggtaa aagcccttgt gaatgctttt cattcatgct atgatagaaa tgtaaatgaa
    20521 aagtaaaatc ataaacacta gagaaaataa tgcagaataa tatagatatt ggagggttta
    20581 ttatatggta agtcagaaag tcgtaatcaa gaatcccacg ggcctgcatc tgcgtcccgc
    20641 cggaattctg tgcaaggagg caatgcagtt caagtcattg atcaccttca aatttcgtga
    20701 aaatactgca aatgccaaga gcgtactcag tgtattgggt gcttgcgtaa aatgtgggga
    20761 tgagatcgaa ttcgtatgtg agggtgagga tgaggcagaa gccctgaaga ccctggtaga
    20821 agccgtagag aacggtctgg gagaataaca ggatcgtcat agaggaagat tacagaggct
    20881 gtgcatattg aagatgcgca gccttttcct atggaactgt cttaagccat ggaaaaaata
    20941 gtttcataca caaaggattt atgctatgct gtaactattc agagcggatt tagaaaatgt
    21001 gattctgaaa agtttatagt acatgaggga gagaaaatta aatggaagaa aaaagcatga
    21061 agaaaagttt ttccagctta ggtttccgat tcctgatcgg aacactggtc atctacgcag
    21121 tgcagatggg agtaatgacc atcgtaggtg cggtgaagcc ggaatggctg caggacacca
    21181 ccataagcct gatcctggca gtattaccac tgtatcttgt gggtatgccg gttctgatcg
    21241 ctgtggtaaa gcggatgccc ggggaagcac ccgcgaagaa aagcattaca ccgggacagt
    21301 ttgttctggc actgatcatg tgttttgcac tgatgtattg tggtaatctg gtgggaacgc
    21361 tgatcaccac tgtcgtaggg gtattaaaag gctccgcagt ggacaatgca ctgatgacct
    21421 atgccaccgg atccaatatg atcgttacat ttctctatat ggtgatctgc gcgccgatcc
    21481 ttgaggaata tattttcagg aaactgatcg tggatcgtac cgtaaaatac ggacagggag
    21541 tagctgttgt tctgtccggt ctgatgttcg gattgttcca tggcaacctg aatcagtttg
    21601 cctacgcatt tttactggga atgtttctgg catttctgta tgtgaagacc ggagaactga
    21661 aagtgaccat cggcttgcac atgtgcatta attttatggg tgccgtagtc agtgtactgt
    21721 tattgaaagc aatccatctg gaggaatacc aggaagtcat tatgaacggg gcagattcac
    21781 aggctgtcat gaactacatg atgaagtacc tgccgggctg gatcggctat atgatctatg
    21841 tattgtttat tctggcagta ttggttacgg gaatcgtatt gttcattgta tatcgtaaga
    21901 agctgaaatt agagccggga cagattgcaa agggacggag attcaagacc gtgatcggca
    21961 atccgggaat gatatgctac tgtgttttct ggatcgccat gatcattatc cagatgttcc
    22021 cggagattgt tacagcaatt acgggaaatc tgtaaaaaga acggctgtgg cagaacatcg
    22081 ccgcaaagga gatcggaagt tatgtacgca tttgacagta gaatacgtta cagcgagaca
    22141 gacagtgaag ggtatcttac gctggacgca ttactgaatt attttcagga ttgttccacc
    22201 ttccattcgg aggatgtggg gctgggaatc ggttatatga aggagattgg tcaggtctgg
    22261 gtcctttcct cctggcagat cgtggtgaac cgttatccga aattagggga aaaagtaaag
    22321 atcgtgacgc tgccctatga gctaaaagta tttttgggat accgtaattt tgccatgctg
    22381 gatgaacagg gagaatatct ggcgaaagcc aattccttat ggagtctgct ggatgtcggc
    22441 accggcaaac cggtaatggt gaacgaagcc atgcataagg gctacgaaat agacgaaaag
    22501 ctggacatgg agtatgcccc ccgcaaaatt gccgtgccgg aaggtggaga actgttggaa
    22561 cccatcgtgg tgaaaaaaca ccacctggac accaaccatc atgtaaacaa tgggcagtat
    22621 gtaaatattg ccatggagta tctgccggat gattttgtga tccgtcagat gcgggcggaa
    22681 tacaagaaac aggcattcct ggatgatgta ttgcatccgt atgtagtgtc tacagagaat
    22741 gactacgtca tctgcctgca ggacgaagcc gggaaacctt atgtatcagt ggaatttcag
    22801 ggacagtaat cctatgctgt cagaacagga gagaacgtgg aattgagatt aggcgaaaaa
    22861 cagacgctgg taatagtgaa aaaagtagaa ttcggagtgt atctggcaac gaaggaagca
    22921 ccggaggaca aagtgctgct gccggcgaag caggtgccgg aaggagcaaa ggtcggagat
    22981 gaactggaag tattcctgta ccgggattcc agtgaccgtc tgatctctac gacgagaacc
    23041 ccgaagctgt gtatgggaca ggtggctctg ctcactgtag tacaggtagg gaaagtaggt
    23101 gctttcctgg actggggact ggagaaggat ctgctcctgc cctttaagca gcagacccgc
    23161 aaggtaaaga ccggagaaca ggtgctggca gccctctata tcgacaagag cggcagactg
    23221 tgtgcgacca tgaatgtcta tgaacatctg cgcacggaca gcccgtacaa aaaagacgat
    23281 aaggtaaccg gccggatcta tgagatcagc aagaatttcg gtgcgtttgt ggcagtggat
    23341 aactgctttt ccggactgat cccgaaaaaa gaactgttcg gagataccga gctcaggatt
    23401 ggcgatcagg tcactgccag agtagtaaaa gtgttggaag acggtaagct gacactgagt
    23461 gtccgggaaa aagcctatct gcagattcag aaggatgcgg agaagatcga gagactgctg
    23521 gacagctatg aaggttctct gccgtttaat gataaggcgg cgccggaggt cattgcccat
    23581 gagaccggca tgagcaagaa cgagttcaag cgcgcggtag gacatctgtt aaaagaaggc
    23641 aagatccaga tcacggagaa aaatattcgc agggtgcgtg gatagtgtgg aaaaaacaaa
    23701 aaaagtgtgc tatagtaaga acgtaatctg agagatatga acagatacag gaggtgttat
    23761 catggatatc gcaggattat caatgtcaat ggcttcaaat aatctgcaga cgaaaatcgg
    23821 aacggctgtg ctgagcaagg cgatggatac gaacgaagca ctgggacagg gaatcgtcca
    23881 gatgattgat tccgctgcta tggaacgcag cgtaacaccg gaacttggtg cgaatatcga
    23941 tctgcggatt tagcgcagaa ataatacata ataaacgcta agacaaaaag agttcggaat
    24001 attagttttc cgaattcttt ttttgcgcgt atttaaaaga aaaaggcatt tttactaaaa
    24061 aatgcataaa aaactattgc atttttacca gtttttctgt aaatttatat atgatatgga
    24121 ctcacatatc atttttcggg gttatagcaa aagagaagta tgtttgggga ctgaaggagc
    24181 agaagatgat atcaaatcag attttacaga acaccattga aggattgaaa ggaatcgcca
    24241 gagtggaatt gtgtgtcatg gatgtggatg gcaaggaagt agcagccacc atggatatgg
    24301 gcaactgttc caagcccgcg gtggaattcg cggcatcacc ggcggactcc caggagatcc
    24361 agggatatca gtatttcaag atctatgatg agcagcagct ggagtatatt ctcgtagccg
    24421 gaggtaccgg tgaggatgtc tatatgatcg gcaaaatggt tgcgtttcag atccagaatc
    24481 tgctggtggc ctataaggag agatttgata aggacaattt catcaagaat ctgctgctgg
    24541 ataatctgct gctggtggat atctacagcc gttccaagaa gctgcatatt cagacagacg
    24601 tgcccagagt ggtcatgatc gtggaatctg cgggaggcaa ggataataat gtcctggaac
    24661 tggccagaac tcattttggc agcaacagca aggatttcat cacagcggta gacgagagca
    24721 acgtgatcgt ggtgaaggaa ttcacagaga ccgataccgg caaagaaatt gaaaaatcgg
    24781 caagagcact ggaagcggct ctcctgaagg aaggtatccg tgagatccgt attgcatacg
    24841 gtaccattgt acatgagatc aaagaagtca gccgttccta caaggaagca aagatggctc
    24901 tggatgtagg caaaatcttc tttgacgaga gagatattat cgcatacagt gaactgggaa
    24961 tcggccgtct gatctatcag ctgcccattc ctctgtgcaa aatgtttatc cgtgagatct
    25021 tcggaggcaa gagcccggat gattttgacg aggagaccct gacgaccatt tacaagttct
    25081 tcgagaacag cctgaatgtg tcagagacct ccagacagct gtttattcac agaaatacgc
    25141 tggtatatcg tctggataag ttacagaaga gtacagggct ggatctgcgg gtatttgaag
    25201 atgccatcac cttcaagatc gcactgatgg tcgtaaaata catgaagtat atggaaactt
    25261 atgagtattg atttcttgcg gagccatgaa ataacggaaa gtgtgcgtcg aatgcttgca
    25321 ttcgtaagaa catttgacgt tatttctttg gcgagaaagc ctcaaatgag caaacaacaa
    25381 gttaacttgt tgtttagaag agcatcgtag atgcgatttt gcgaatgagg ctctgaatag
    25441 ggatcaatat ggtagcacaa aggcagcaac tgtccgcagg gtgggatctt acgggcaata
    25501 gaaaggcatg atcataaaat gacaagaaaa gaaacacaaa ggctgaaaag agagcggatc
    25561 attgaggaaa acggaatcat tttcctggac aacgtcagca agtcttattc ctcaggttct
    25621 ccggcactga atggtgtgtc gttaagcatc ggaagagggg aatttgtatt tattgtaggt
    25681 gacagcggat ccggtaaatc cacgttgatc aaattactgc tgcgggagct tacagcaacc
    25741 agcggcagtg tttatgttat gggaaatgat ctggcgaaat taaagcacag gcagatcccc
    25801 cgttttcgtc gtaacctggg tgtcgtattc caggatttcc gactgctgaa tgaccgcaac
    25861 gtctatgaaa atgtagcatt cgcacagcgt atcgtagaga ctcccgtcag tgagatccgc
    25921 agaaatgttc ctgcgattct ggcaactgtg ggactggcag gcaagtataa ggccaagacc
    25981 aaacagctgt ccggcggtga gcagcagcgt gttgcactgg ccagagcgct ggtgaacaat
    26041 ccttccgtac tgttggcaga tgagcccacc ggtaatctgg atcccaataa ttcctgggag
    26101 atcatgaaat tgctggagga gatcaatgag agcggcacca cggtagtcgt ggtcacccat
    26161 aaccgcgaaa tcgtcaatgc catgcagaaa cgagtgatca ccatgaaaaa aggtgtgatc
    26221 atcagtgacg aagagaaggg tgggtatatc gatgaagatt agtacacttt tttatacgat
    26281 caagcaggga tttgccaata ttttccggaa taaatggtat tctctggcat ccattgccac
    26341 gatatccgca tgtctgtttc tgtttggact gttctattgc atcgtggcca actttcagaa
    26401 tatcctgaag actgcagaag aaggtgtatc cgttacggta tttttccaca gtgatatgga
    26461 taactgtgag agtcatgtag acggacagat cccgtccgaa cagcgcctgg aagagatcgg
    26521 acaggagatt gccaagcgtg cagaggtatc cgaggtacag ttccagtctg cagatgatgc
    26581 atgggcaacc ttcggaccgg attatttcgg tgaggattat gcagaaggat tccccgagaa
    26641 tccactggca ggagaggata cttatgagat tttcctgagt gatgtctcca tgcaggatgc
    26701 actggtgacc tggctacagt ccattccgga ggtgcgcaag gtcaactatt ccgaaatgac
    26761 agccaacaca ctgagcggtc tgaaccttct gatcgcctac gtgtccatgg gaatcatcgt
    26821 gatcctgctg gcagtcagca tcttcctgat cagcaatacc gtagctatcg gtatttctgt
    26881 ccgttctgcg gagatcaata ttatgaaata tatcggcgct accgactttt tcgtgcgtgc
    26941 gccctttgtg ctggaaggta tgctgatcgg tctgatcggt gcagcagtac cgctgggact
    27001 gatctattcc ctttacaatt atgcgttgaa ctatgtgatc aaccgtttta tggtactcag
    27061 cggcttcctg aatttcctgt ctgtggacga gatattccat ttcctgatcc cggtatctct
    27121 gggtgtcggt gtgggtatcg gtttcctggg aagtatcgct actgtaagaa agcatctgca
    27181 cgtgtaaata caattccagc agataacccg gatcctattt gtcatgatgt gtgcggagtg
    27241 tggctctgca ctgcatcatg acattttaag aatggagact aaatcaaatt gaataaaaag
    27301 aaaaaaggct ttctgtgcct ggcactgtgc ctgatcctga cggtttcctt cggcatgcag
    27361 ggattgcggg taaatgcgac caatatgtca tccattacct ctgacagtat ccgggacaaa
    27421 caggatcaga tcaaacaggc ggaaaaagaa cgtgaacaga tgaaaagcaa tctgagcaat
    27481 ttggagcaga tcaagaaaga cctggagaca cagaagacca atctgaaaaa ttatgtaacc
    27541 cagctggatc agaatctgag ccagatcgaa cagaatattg cggatctgaa aaataagatc
    27601 acagcaaaag aagaagaaat tgcaaagacc caggcggaac tggatgcggc actggagaga
    27661 gaagaaaatc agaaagatgc catgatccgc cgcataagaa ttatgtatga gaaaggggat
    27721 tcctacatcc tcgatatgat gttgaaagca gaaagcttca gcgatttcct gaaccgggcg
    27781 gatttcatgg atctgatcat ggcttatgac agacagcaat ggaaagactt catggagaac
    27841 cgcaaatata ttgaactctg caaagaggaa ctggaagcgg agaaagccat tctggatgag
    27901 gccaaggccg gcgtggaaca ggagcaggcc aatatggagg ctctgatcga ccagaagaac
    27961 cgggatatca ccgcttacga atccgatatt accaacaagg aacaggccat caaggaatac
    28021 aagcagtcca ttgcggatca ggatgcggag attgcagccc tggaggctgc cattgccgca
    28081 gagaaaaaaa agatcctgga ggccagtggt actgtgctga cttacgatgg cggtaccttt
    28141 aagttcccgc tggcaaccta tacccggatc tcggatgatt acggcaaccg tatccatccg
    28201 acactgggaa tcgaacagtt ccacaacggc gtggatttcg cggcgcccaa gggcacggct
    28261 atttatgcgg cctatgacgg acaggtggtg gcagccactt acagcaacac catgggcaac
    28321 tacgtgatgc tcgaccatgg tggtggactg tatacgatct atatgcatgc ctcggcatta
    28381 tatgtatcca aggacgatat tgtggtcaga ggagagacga tagccgcagt gggctccacc
    28441 ggacgttcca ccggcaacca tctgcacttc agcgtgcgta aggatggagc ttacacatcc
    28501 ccctggaact acttgtcgca atagttctgt aatcgcaggg cacgtgactg cttgcaggca
    28561 aagtgctctg gcgattaaca gaacaaacat acatgagcaa gtagggcgat agcccggaat
    28621 gcgaatgtat gcagcatttc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28741 natgcgaatg tatgcagcat ttcgggagtg cggagcacgg agaaatgcgt gttgcgacaa
    28801 gaagcaggac gtgttgcgac aaaccaatgt ggagaagcgc cgatagcccg gaatgcgaat
    28861 gtatgcagca tttcgggagt gcggagcacg gagaaatgcg tgttgcggca agaagcacga
    28921 cgtgttgcga caatatcttg gaaataaagg agagcattta tggaggaaaa taaggaactg
    28981 gaaaccacca ccgaagaaca ggcttctgta actccggcgg caaataatgg cgagaaaaaa
    29041 ggcttatttc tcagtggcct tatcgttggc tttgcggcca cgttgctgat cgtgggcggt
    29101 atctttatcg cttttcagat ccacaacatc atgaccatga aggatgatgt ggcagcacag
    29161 gtatcctatg gagaagattc tgcggtcaat gcacagacca ttaaaaaact gcaattgctg
    29221 gaagacactg tgaaggagaa cttttatctg agtgaggtca ccaatgaaca gctggaagac
    29281 ggtatgttcc gtggcctgat ggactctctg gatgacccgt attccgagta ttataccgca
    29341 gaggagctga atgaactgac ggaacagaca cagggcattt actttggcat cggtgcctat
    29401 gtcagcatgg attccgagac caatctgccc aagatcagcg gcgtgatcga aggagcaccg
    29461 gcagctgatg tggatctgcg tgccaacgat attatctatg aagtggacgg tgtctccact
    29521 gcaggtaaga cgttgacgga agcggtgaat atgatccgcg gagaagccgg gaccacagtg
    29581 gatctgacca ttgtcagaca gggcgccggg gattatctgc acgtcaccgt ggagagagcc
    29641 aaggtagaga gtcccaccgt aaaatacgaa atgttggatg acaatatggc atatatccag
    29701 atcgtggaat tcgacgatgt gaccatagac cagtttaccg atgcgctggc aaccgtgaag
    29761 ggctccggca tggaaggcct gatcctggat ctgcggggca atcccggtgg aagtctggat
    29821 gcagtggtta aaattgccag aaagctgctc ccggaaggca tgatcgtata taccgaggac
    29881 aaggcaggca agcgcacaga gtatacctgt gacggcaaga ctcctctgga agtacctctg
    29941 gtggtactgg tggatatgaa cagtgccagc gcctccgaga ttctggccgg agcaatccag
    30001 gactacggcg ttggaaccct ggtaggtacc acgaccttcg gtaaaggaat cgtacagcag
    30061 atcatgccct tcaccgacgg atccgccatc aagatcacca tcagcgctta ctatactccc
    30121 aatggccgca atatccacgg aaccggtatt gaaccggatg tggaatgcga gtttgacggg
    30181 gaagcatact acaacagtga tgatccggtg gacaatcagt tggagaaggc aaaagaggtc
    30241 ctgaaagaat taatgaaata aaagtcttga caaagcgcac aaaactgtat aatatgtaac
    30301 ccataaaaac aaaggcgttt ttaccggatc ggtaaaagcg cctttttctg ttatcaaaaa
    30361 ttcctgcccg gaacgtagaa tggaggagca aaatgatcag actggaaaat gtatgtaaga
    30421 atttcggaga tctccaggta ctgaaaaatg taaatctgga agtaaaagaa ggcgagaagc
    30481 tggttatcat cggaccctcc ggttccggta aatccaccac cgtgcgatgc atgaatttcc
    30541 tggaggagcc taccagtggt caagtatata tcgatggaca gaagctgacc catgcgaaca
    30601 agaccaagat catccgggag tccatctcca tggtattcca gcagttcaac ctgtatccgc
    30661 atatgacggt attgaagaac ctgaccatag cacccatgaa gctgcatcac aagagcaaaa
    30721 aggaagccga ggatctggct tatcattatc tggatgtggt aggacttagg gataaggctc
    30781 atgtgtatcc cacaacactt tccggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30901 nnnnnaaatg gactgcattg gcagcagtca tggcaatgac tgcaacttta tttaccggat
    30961 gcggcagcac tgatgccccc gcttccgggg caacagcacc tgcagcggaa agtaccgcag
    31021 cagcaaccac agaaacagga gcggaagctc ctgcagcaga cggcgagctg gcagcagacg
    31081 tacaggcaat cgtagaccgt ggtgtattga gagtcggcgt taaaaatgca gtagtaggct
    31141 ttggcttcca ggataccctg accggtgagt attccggact ggagatcagc ctggctgaga
    31201 agatcgcaga gagccttggc gtggatgtag agttcaccgc tgtaaccgca gctaccagaa
    31261 ccgagcttct ggattccggc gatattgact gtgtactggc tactttcacc atcaccgatg
    31321 agagaaagca gagctgggat ttctccactc cttattttac agactatgta accgtactgg
    31381 tagaggataa gagcggcatc agctccctgg cagacctggt agacaagaag gtcggtgtat
    31441 cctccggatc cacttccgca aaggcactgg taaaggctat gatcgatgca ggcctgatcg
    31501 atggcagcgg ctttgatgca gatacctttg atcccgcact gtggactacc ggaatctctt
    31561 tccagcagta tgatgactac cctgcaatct ccaccgcact gagtgccggt gaagtagatg
    31621 ccttctgtgt agataagtcc attctggcta tttacaagac tgacggacgt tcctacatcg
    31681 acgacaagtt ctcccctcag gaatatggcg tggctaccac caagggttcc ggcttctctg
    31741 catacgtgga tgaactggta cagggatggc tggcagacgg tactattgac agtctgatca
    31801 cagagaacgg attagagtaa aaaagaaaaa agtaactggg aggaaacact atgtctggta
    31861 ttatttcagc aagagcctgg ggcaaggtct gggcgtacag gagcacattc ctgttaggac
    31921 ttctgaatac tgtggagaca gcattttttg cgatcctgtt atcgctggca ctgggtgtga
    31981 tctttggcct gatggctacc agcggaaaaa aggtattgca gatcatcagc agagtctatg
    32041 tggagatcat gcagaataca ccgattttgc ttcagctgtg tttcttctac tatgcgctgg
    32101 ctttttccgg tcacagtatc ggaatcctgc cgacaggtat cgttgcactg ggcgtataca
    32161 ccggagccta tatggccgag gtggtgcgcg cgggcatcga ggccgtccct aagggacagt
    32221 tcgaggcggc tgtgtcccag ggattcactt atgtggagcg gatgtattat attatcctgc
    32281 cccagagcat caagatcatt ctgccgccca tggtgaacca gatcgtaaac ctgatcaaaa
    32341 atacctcctg cctgtatatt atcggcggag cggatctgat ctcgctgact tacagctttg
    32401 tcaccggtga aaataccggt ggagcctatg ctccggccta tctggtcagt ggactgttat
    32461 ttttcgttat atgttatccc ctctccaaga cagccagtgt ctgggagatc catctgaaga
    32521 aaagagacca gcgggtgaca tttcagagaa cagaagggac ggtgacggac aatgagtaac
    32581 ctggaagcaa gcttttccat actgggacag aaaggtgtta tggcctacat tctcaaaggt
    32641 acggtattca ctctgatcat tgccctgatc gctgttgtgc tgggaatcgt catcggttcg
    32701 gtactggccc tgtgcagaaa ctactgcaca agcaaaaaga cgaaaatatt tggcatgatt
    32761 gctaccgtat acatagaagt atttcgaaat acaccgttgc tgctgtggat cttcatatgc
    32821 ctggtattct gcccctgtcc ggagctgttc aaccggaaac tgttcgggct gaccacagta
    32881 gagaccaagc tgttgttcaa ggcagcagtt gctctgatcc tgtttacctc ttccgttatt
    32941 gcggagatca tccgcggagg actgaattcc atcgcacatg gacagtttga agccggccac
    33001 gcacagggat tcaatgtggt acaggttatg atctatatta ttctgccgca ggcttaccgg
    33061 aatgtcattc ctaccctgtt gagtcaggtg atcaccacca tcaaggactc ttcctatctg
    33121 gcaaatgtgg cgaccatcga actgatggcc cggaccaaac agctgctttc cgcagccagc
    33181 cgttacaatg gtactggtaa tgtaaacgta tcggatgtat ttgtgctgtt tggtgtagct
    33241 gcattgatct attttatcat caactacaca ctgtcctgcg tggtacgtac catgcagaaa
    33301 cggcggaaga gaccggtacg tgtgaccgca gattaaacga gaaaagaaaa gaggatatcc
    33361 atggaacagt atgtgatcac gatttcaaga caattcggaa gtatgggacg ttccattgcc
    33421 agggaactgt ccgaaatact gggaatagaa ttcctggaca gagatattgt ggaggctacg
    33481 gcgaagcgca tgggccttcc ggtatctgtc atcagcgatg aagaagagag tatgaagtct
    33541 actttcttcc ggaggcagta tccgctcgga atgggaatgt ccagcctcaa ggatgagatc
    33601 tttcttaccc agaaaaatat catccgggat ttcgcagcca agggatcctg catcattgtg
    33661 ggacgctgtg cggactatat tctggaggat atcccgaatc atctgaatgt gtatgtctat
    33721 gcccccgatg aggcaaggct gaaaaactgt gtggagaacc tgcagatgga tccccagacg
    33781 gcaaagaaaa tgatgcggga tgtggatgct gccagaaacc gttaccacaa agcctatatt
    33841 cccggctggc agagcgtttt cgaccataag gatctcatga tcgacagcag ccggttcggc
    33901 atcgacggca cagcaaagat tctggcagac attgttaaaa accagtgggg ctgatggtcc
    33961 aaagattttt gatctgcgga gacagcgtgt cataatccca ggtgcgggaa gaattgctta
    34021 cccgattggg atctttgacg caaaagcccg tgtcatcata atcatagagc aggatatagt
    34081 ggccggaggt ggtgaaatcc cccggccgca tgctgcatac gataagtttg ccctgatcca
    34141 gggctgtttt catgctgttt tcaaccaggg gaagttcctt gccggacagg ccgagggcag
    34201 gaagcccttc ggtccacagg gaccagctgg tgccattttc cgtataataa ccgttctttt
    34261 cacagtagga ggcaatctct ctcggggtgc cgttcaaatc gtgggtgaaa tagacataga
    34321 ccatggacag gcaggtggga ccacagccgg cgagaccgat gatgctatca ccgtaggact
    34381 cgtagcccca gcgcttgtcc cattgcagga gcagaggcac ctgtccaatg agaacatcgg
    34441 aagcaagatc gatggaccgt cccagataat tttgtctgtt ggggtagtct gccacgaaat
    34501 ccgcggcttc ggcattattt tgggcaagct cggccagtgc ttccgtgatg tttacggtat
    34561 ctgcggtggt agagagactt ccggcagcgt cagattcatt ttcttcccgc agtgcagcaa
    34621 aatcatattg gacaaccggc agatcatacc gggcagtcag ctgataatac agatgattga
    34681 cggcaggaag aatctcatgg ccgtaatcct gccagaaaga gactgctgtc aggagcaaca
    34741 cgatggcaaa tgagacccgg aggaagcaga ctatgcgttt tcttctgcgg atcgcccgct
    34801 tcctgcggag catctcctgc ctggaaattc tgcggcggcg ttgttgcacc ggaacctgat
    34861 aagctgcatt tgcattcata gaaatccctc ccgttagaac ctttttgaca aaataaccgt
    34921 cttttctata gaagagtata aaggattgtc ccataaaaag tcttttaagc agttttaagc
    34981 tttttgacag atatctttaa gaattcatga agatatgtta caataaagca tggaccaaaa
    35041 tcattcggaa aggggaaggg accgatgatc aggagaaaat atgttccgct gcgcattgtg
    35101 cagccgggga tgatgatcga tcaggcaatc atagaccgtg caggccgtgt actgatcgcc
    35161 agacgtaccc gactggagga ttt
//
LOCUS       DAWEJW010000016        34465 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_552_length_34465_cov_71.917350, whole genome shotgun sequence.
ACCESSION   DAWEJW010000016 DAWEJW010000000
VERSION     DAWEJW010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 34465)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 34465)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..34465
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_552_length_34465_cov_71.917350"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         9486 a         7374 c         8867 g         8738 t
ORIGIN      
        1 ctaactttct atttacagct ttgtggtcca ctgggacaga tcccatacct gggtggccag
       61 gccatcgtag aattccggct catggcagac catgagaata cttcccttgt attctttcag
      121 tgcccgtttc agctcgtctt tggcatccat atccagatgg ttcgttggct cgtccagtaa
      181 cagaacgttc gtggcacggt tcatgagctt gcagagtctt acctttgcct gctcaccacc
      241 gctgagtacc tttacgcggc tctcaatatg ctttgtggtc agaccgcatc tggccagggc
      301 ggaacgcacc tcatactggg taaacgcggg aaattcctgc cagatctctt cgatacagct
      361 gttgtcaccg gaaccggaca tttcctgctc gaaataaccg atttccagat aatccccctg
      421 ctccacttcg ccggacagag gcgggatcag tcccagaatg ctcttcaaca gtgtggtctt
      481 accgataccg ttggcaccga tcagcgctgc cttctcgcca cgttccatgg acagattcag
      541 gggcttggac agcggctcgt cataaccgat gatcagatcc tttgtctcga aaatgatacg
      601 accagtggtg cggccctcct tgaaagagaa ttcaggcttc ggcttctccg cagccagctc
      661 gatgacatcc atcttatcca gcttcttctg gcgggacatg gccatgtttc gggtggatac
      721 acgggccttg tttcttgcta cgaaatccct caaatcggcg atctccttct gctgacgatt
      781 atatgcggcc tccagctggg atttcttcat ggcatagact tcctggaact tgtcgtaatc
      841 gcccacatag cgggtcagct cctgattgtc catgtgatag atcaggttga tgacactgtt
      901 caggaaggga atgtcatggc tgatcaggat aaatgcgttc tcatattcat tcagataacg
      961 cttgagccat tcgatatgtt ccgcatccag atagttggtc ggctcatcca gcagcagaat
     1021 atcgggcttt tccaacagaa gtttgcccag cagaactttg gtacgctgac cacctgacaa
     1081 ctctgtcaca tcacggtcca ggccgatatc catcagtccc agagctcttg ccacttcctc
     1141 cactttggca tcgatcatat agaaatcatg catagtcagc agatcctgga ttgttcccag
     1201 gtcttccatg tactgttcca gttcttccga ggtggcatct cccattttgt cacagatttc
     1261 attcatctgt gcttccatat cgtataagaa atcaaaagcg gaagccagta cctgacggat
     1321 cgtcataccg ggggtcagca ctgtatgctg gtccagatag ccgacgcgga cattcttcgc
     1381 ccattccact ttgccctcat ccggcatcag tttaccggtg ataatattca taaaggtaga
     1441 ttttccctcg ccgttggcac cgatgagacc gatatgttcc cccttcagca ggcgaaacga
     1501 tacatcgtta aaaatagcac ggtctccaaa tccgtgggtc aggtgttcta cgtttaatat
     1561 gctcatattt cctcacattc tgctgctttg cagcgtcgtt tccatcaata gtcctttgca
     1621 agtatagcgt actgtccgta gttttgtaaa gaaagaaagc aaaaagggtg gaaaacaata
     1681 attttttgtt gtgaaaaaga gcacatatgt gctatgatgt acgcggtttt gcgaatgggg
     1741 ttctgaatag ttactatttc ctgattaaat acagaaagaa ggcagcgtat gcttttactt
     1801 caacaaatgg tgattttttt cctgatcatg ctgatcgggt acatctgcag aaaggtcgga
     1861 gtattcgggg aaaataccgg caagatgatc tccggtatcg tcattaatat cggcaatccg
     1921 gcgctgatca ttgcctccgg catgaatcct gaaacattgg aaaataaggc ggcattgttg
     1981 gttacgctgg ctgtggcgct gatctttttt gctatcatgt ttgtgattgc agagctgatt
     2041 ccgagactgc ttcgggcaga cagagaagat tatggttctt atcaggtcat gacgatcttc
     2101 gccaatattg gctttatggg ctatcctctg ctggatgcca tgtacggcgg tgaagcagtg
     2161 atccatgctg caatttttaa tctgctttac agtgtgctga tctataccta tggcattaac
     2221 aggatgaaga cctccggaca gcgggaaaag ctgaactgga agcagctgat gaatgtgggg
     2281 gtgatctcct gcctcattgc agtgaccctg tatatcagta atcttccggt tccgatggtc
     2341 ttcaaagata ctgccacccg gatcggagct gtcaccggac cattaagtat gctggtaatc
     2401 ggtgattcgc tggcacagat ccggttaaag gatctgttta cggatgtgcg gctgctgctc
     2461 ttttctgttg tgaagctgct gcttatgccg gcactgcttc tgtggggact gcgttttttc
     2521 attacagacc ccatgtttct gggtgtctgt ctggtcatga cggccacacc tgtcggcagc
     2581 atgacagtca tgctggcgca gcaatatgac ggtgattacc ggctgacctc taagggcgtg
     2641 gcgctgacca ctgtgctgtc cgtagctaca atgccgtttc tgttctggct gttgaaaatt
     2701 taatgaaact ttttctaccg gattgtcgtc tgatagaata gggatcccca aaaaggggat
     2761 actgcaactg aacatgggaa atatccctgc aaaacaagga gagattttat gaaaaagaaa
     2821 ttagcatgta tgttacttat gagtgctctg gcactttcta tgaccgcctg cggaggcaag
     2881 gataataacg gtgctgcatc agacaaccag cagtccacca tggagagcac cgcagcggga
     2941 caggagaccg gcgaggccgg tagtacaacc gctgcacaga atccgaagga cgctgcgaaa
     3001 gcgttacaga gtgcaaaaaa ggcagttacg gatgcactgg gagacaatta ctggccggat
     3061 tccgagatac ccgaggaaat gctgaacgag acctacggtg tcagtgcgga gctctatgat
     3121 gcctacctgg gagaggcacc catgatcagc gccaatgtag atactttatt gattattcat
     3181 gcaaaagatg gtaaggtaga agaggtagaa aatgcattga acgcctaccg tgacagtctg
     3241 gtgaatgaca cgatgcagta tcccatgaat ctgggcaaga ttcaggcatc ccgagtggaa
     3301 cggataggcg attatgtatg ctttgtacag ttgggtgcgg acacttcctc tgtggaagaa
     3361 cagggcgatg aggccgtgat cacttactgt caggagcaga atgaacttgc cctggaggct
     3421 atcagacaga ctctggagta aatacggagt agagtgaatg gtattcagca gtgtattgtt
     3481 tctttttcgg tttttgccga tttttatgat ctgctatttc ctggttcccc ggaagatgaa
     3541 gaatctggtt ctcttcctgg ggagcctggt cttttatgcc tggggagagc ccgtctatat
     3601 tttcctcatg cttttttcta ccatatccga ttatgtgtgg ggaaggctga tcgaagaata
     3661 cagagggaag gatcactcca ggatcttttt gctatgttcc attggcatca atctttttat
     3721 ccttggattt tttaaatatg ccgatttcct gatgcagacg gtgaatacag tatttggaac
     3781 atcaattccg ctgttaaagc ttccgctgcc catcggaatt tccttttata cgttccagac
     3841 catgtcttac gtgatcgatg tgtaccgggg cgatacaaaa gcgcagcgta atattctgca
     3901 gttcggtgtc tacgtgacca tgttcccaca gctgatcgcc gggcccatct taaagtatca
     3961 tcaggtggaa cggtatctgc aggacagacg gaccgacctg gatgccatca gttacggggt
     4021 aaaacgattc gtgacaggac tggccaaaaa ggtactgctg gccaataacc tgggactttt
     4081 gtggaaacag gtaacggaac tgggaaatga gcagatgagt attctgatgg catggctggg
     4141 aatcgcagcg tttgcactcc agatttactt tgatttttcg ggttattccg acatggcgat
     4201 cggattgggg gcagtcttgg gcttccattt tccggagaac ttcaattatc catacattgc
     4261 tgcttcggta aaagatttct ggcacagatg gcatatttct ctgagtacct ggttcaagga
     4321 gtatgtgtat attccgctgg gcggcaatcg caaggggctg ccgagacagc tgtttaatat
     4381 tctggtggta tggatgctga ccggtatctg gcacggtgca ggatggaatt tcctgttctg
     4441 ggggctgtgg tttgcatttt tcctgatcct ggagaaatta ttcttaggag atattctgga
     4501 aagtgtgccc aaggtgttcg gcaggatcta tacgttggcg gtagtactga tcagctgggt
     4561 gttttttgca ctggaaagtc ccggagagat cctggcttat ctccaggcta tgttcggaat
     4621 gaacggaata gggccggtga attcgctggc aatgtttttg tgcaatgagt atctggtgct
     4681 tctggtcatc gcgctggtgg catgtcttcc gctaggcagc agactggtgc atgctttgaa
     4741 aagcagtaag acaggccccg ccatggcact gtaccggttg ggagaaaagg tgattccggc
     4801 agtactgttg cttctgtcgg tggcgtatat tgtggatgcg tcgtataatc cctttttgta
     4861 ttttcgattc tgaccgggaa ggaggaaccg catatggatg ataaaagaaa ttcgctgttg
     4921 accatcggac tggtctgtgc ggtacttctg gttctgggag tggcagatct gtggaacagt
     4981 gacagagtat attcggaagc ggagaaccgg gtgctggcat cccgccccgt attttcctgg
     5041 gagagtcttt tcacgggaga atacggggat gcttatgagg aatacatgtc cgaccagttc
     5101 gtaggacggg ataaatgggt aggacttaag accggggcag atatccgttt ccggaaaaaa
     5161 gagatcaatg gggtctatct gggagcggat cattatctga tcggagtgaa cgatcccggg
     5221 gaatacacgg aacagatgga gaactcccgt gtggcatcgt tgacaaaact ggtgaaccac
     5281 tgggatgcca aggtgatgct ggtgccgacg gcggacaata ttctcacgga taaactgccg
     5341 gcatttgcac cttattatga cgaagaacgg ctgcttacga aggtaaggaa ttctattgga
     5401 gaagagcatt atatagatgt gtatcatgct ttgcaggagc acgcacagga acccatctac
     5461 tatcgcacgg atcatcattg gaccagtctg ggggcatatt acggttttct ggcctgggcg
     5521 gacagtgtgg gacgtttccc ctacccgtat gacgtaaacg gcatgaagac ggtgtcggag
     5581 aattttcagg gaaccctgca gtcccgtatc aatgtggact ggacgaagga tagcatacag
     5641 tatttcccgg agacggagaa aaaagcagta tcggtgacgt atgattttgc ggacacggct
     5701 gattctctgt atgcaccgga ttatctggat accaagaacc agtatggatt tttcctgaat
     5761 gacaatcatg catttattga gattcatacg ggatacaatc ccggtaagac gctgtttgtc
     5821 atcaaggatt cttatgccaa cagcctgatt ccgctactca cggcccatta tgagaatatc
     5881 tacgtggtgg atctgcggta ttttaacggg aaactgttcc aactgatgga gcagtatgaa
     5941 ccggaacagg gaatggatgt gctggtgttg tatgattgta ttcatttttt agaggatttt
     6001 aagtattatt aaggaggctt attatggctt atttgggcat tccccagaat accattgcgg
     6061 tactgcagaa gtaccatttc aattttcaaa aaaaattcgg acagaacttt cttatagaca
     6121 cgacggttct ggaccggatt atttcttctg cggagatcac gaaggaggac tgcgtgctgg
     6181 agatcgggcc gggcatcggt accatgaccc agtatcttgc ggagcgcgcg ggcagcgtgg
     6241 tggccgtgga gatcgacaag gcactgatcc ctattctgga agagacatta caggattatg
     6301 ataacgtcac ggtcatcaat gacgatatcc tgaaagtgga cattaaccgg ctggtggaag
     6361 agaaaaacgg aggacgcccc atcaaggtgg tggcaaacct gccctattac ataacgacac
     6421 cgatcatcat gggcttattc gagagccgtg tgccgctgaa gagcatcacc atcatggtgc
     6481 agaaggaagt ggcggaccgt atgcaggtag gccccggcac gaaggattac ggcgccctgt
     6541 ctctggcggt gcagtattat gcaaagccgg aaatcgtggc gaatgtaccg cccaactgct
     6601 tcatcccgag accgaatgtg ggcagtgcgg tcatccgcct gacccgctac gaagaaccgc
     6661 cggtacaggt gaaggatgag aagaagatgt tcgccctgat ccgggcttcc ttcaaccagc
     6721 gccgtaagac cctggtgaac ggtctgggaa atgcgggcct cccaggcatc accaaggaat
     6781 ccgcggcagc agccctggca cagatgagcc tgtcccctac cgtaagaggt gaggcactga
     6841 cactggagca gtttgcacga ctgagtgatc tgttgtagag cgacttatca tgaagcagac
     6901 aaggagtata cgttcagaca gctgcagatt cataggtcac tccgtctccg accgataata
     6961 attccgatat tttaacggtg tcatattccg gtatttttta aacacccgtt caaaataagt
     7021 aatattttca tagcccaact gttccgcaat ggaagtgatg ctgtcatccg tttccagaag
     7081 gagccggtgg gcttttttga tgcggttgac attcaggtaa tcattgatgg taaaactggt
     7141 gacctccttg aagatgcggc tcaggtaaaa tttgttgata tagaaccgcc cagcgagatc
     7201 ctccagagag atccgctcag tacaatgttc tacaatatag tgcgccactt catcgacctt
     7261 ttgatacttc gggttcgaat gtctggccgg ggatgagaca acgggaccct cgggctgcaa
     7321 gcgtagcacg gcaatgaaaa attctgccag cttcgtcatg gcggccagaa gatagccggg
     7381 ctttttgtgg atcagctcag aggcaatgtc ctgcaacaga gactggatat aggggcgcat
     7441 ttcttctggc agtgtcagaa tactgccctg ctccgtgaaa aaactctgca gctgcaatcc
     7501 tccgaaagat gccagaaagg tagaaaaggg ctcttccatg attcctaaaa cgatccggtc
     7561 gtgatgtggt ccggcggaag ccataccggt gcggtggatc tgctccttgt caatgaagac
     7621 cagcgttccc ggggtgacca gataggtctg ctggttgata aaataatacc gttccccctc
     7681 taaaagataa taaatctcgt attcttcgtg aatgtgacgg atatccatgg aataattata
     7741 gtcccgtaca acacggtcga tgtggaatcc gtctacaagc tcattataga gccagtgatc
     7801 ctgaggcgtc gaagcaacag cattgtccgc aacgcctgat tccggtggag ccggtgtctg
     7861 aaaatcccct gtttcaaaca taatagtgat ctcctttttc ctatgctata ttagtgctat
     7921 attagactgc attctgaaaa acagtatgcg caggctatat tacaaatcta ttggcttgta
     7981 taaaaacaaa agtcaatata gtacgaaaat ggcaaaaaat acgcaaataa tagcaagatt
     8041 attatacagc cataggctat aatgtgcaat atagagaagg cagtacgggc cgatggagca
     8101 gaggatccca aggaccggaa acacataagg aggatacaaa aatggagtat gcagacggaa
     8161 aggtaaaaga tttacagatc gcctatattg gcggtggttc cagaggatgg gcatggacat
     8221 ttatgacgga tctggccatg gatgaagaac tgtccggaac catccgcctg tatgatattg
     8281 atgcggaggc ggcaaaacac aatgagatca tcggcaacag actttcttca agagaggatg
     8341 tggtgggcaa atggaactat actgtttctg acagcttaca gtccgcattg accggtgcgg
     8401 atttcatcgt gatctctatt ttgccgggaa cttttgatga gatggctgtg gatgtacata
     8461 tgccggaacg actgggaatc tatcagtctg taggagatac cgcaggcccc ggcggtgcca
     8521 tgagagcact gcgtacgatt cccatgtatg tggagatcgc acaggccatc agggcatatg
     8581 caccgaaagc ctgggtcatt aattacacca accccatgtc cctgtgcgtg aagactttgt
     8641 actatgtttt tccggagatc aaagcttttg gatgctgcca tgaagtattc ggtacccaga
     8701 aagtgttaaa gggtattttg gaagagacca tggggcttaa ggatgtgaaa cgtgaggata
     8761 ttcaggtcaa tgtactgggt atcaaccatt tcacctggtt tgactatgct tcctataagg
     8821 gaatcgatct gttccccatt taccggaaat ataccgagga gcataaggaa gacggctaca
     8881 aagaagcaga taaaaactgg gcgaactcta ctttcgagtg tgcccatatt gtgaaatttg
     8941 atctgttccg caaatatggt ctgatcgcag cagcaggcga caggcatctg gtggaattca
     9001 tgcccggtgt gggtgacagt tatctgaaag atcccgagac ggtaaagaga tggaaattcg
     9061 gtctgactac cgtagactgg agaaagaagg atctgcagga acgtctggca aagagcgcga
     9121 gactggcagc cggtgaggaa gagatcgagt tgaagcctac cggggaggaa ggtatccagc
     9181 tgatcaaggc actctgcgga ctgacccgca agatcagcaa tgtcaatatt cccaataccg
     9241 gacatcagat tgccaatctg ccggaaagtg cggttgtaga gaccaatgcg gtattcgaga
     9301 gagatgccat ccgcccgttg tatgccggag agcttccgga gcagatcaag gagctggtgg
     9361 atccccatgt ggcaaaccat gagcggattc tgaaagcagc tattctgtgt gattttgatc
     9421 tggtggtgga agcattcctg gaggatccgc aggtgaaagg aagagcttcc agagaacagg
     9481 tggaagagct ggtaagggat atgctccgca gcacggcaaa atatctgccg gaaggctgga
     9541 aaaagtatct gtaaaaataa aatagaaaaa agaacccggg aggtgctgca gaaggatgag
     9601 gcggcacctc tttttgtgta tacgatgagc caggtacaga tttgtgcttg cacaagaatc
     9661 tgtacctggc gaatgtacat catcgagctt ttaagcgaga tggtgtacaa agtataaaaa
     9721 tcgagtagga gacagtccgc tcgaggggtt aattatttga aaaaagaata taagcggcag
     9781 aagaattata aagtcattgt gaaaatgcac aaaactgcga gaagtatttt gagaacaata
     9841 tacaaacttc cagggattta cagaaaagca cttgcaacca gttcgtgaat gtgctatttt
     9901 ctaattacag caacggaaac gttaccggga acaataccga aaccgtttcc gacaacctga
     9961 cgggagtata gcaagaatta tgaagggttc ccgttccaac aaaaaaggag aggtaagatt
    10021 atgaagaaga aactggcaag tgttttactt tgtgcagtaa tggcagcttc catgctgacc
    10081 ggttgcggaa atgctgacaa tgcaacagca accacccctg ctaccgacaa caagacggac
    10141 gcagcagcaa ctaccgaggc ggcagagaag accgcagagg cagcaggctc tgtatactat
    10201 ctgaacttca aacctgaagt tgacgaagta tggcagaagc tggctaagga atataccgag
    10261 gagaccggtg tccctgtaaa ggttgtaact gcagcaagcg gtacctacga gcagacactg
    10321 atgtctgaga tcgctaacaa ggaagcacct accctgttcc agatcaacgg acctatcggt
    10381 tatcagaact ggaaagacta ctgcgcagac ttaaaggata cagacctgta cagctggtta
    10441 atggataaga gcctggcaat caccggcgaa gacggtggcg tatacggaat cccttacgta
    10501 gtagagggtt acggtatcat ttacaacgac gctatcatgc agaagtactt cgcactggat
    10561 ggtgctaagg cagcaagcat ggatgagatc aacaacttcg ctaagttaaa agaggttgta
    10621 gaagatatgc aggctaagaa agatgagctg ggtattgaag gcgtattcgc ttccacatcc
    10681 ctgactcccg gtgaagactg gagatggcag actcatctgg ctaacatccc tgtatactac
    10741 gagttcaagg acaagggtat caccgatacc gacactctgg agttcactta ctccgataac
    10801 ttcaagaaca tttttgatct gtacatcaac aactcctgca cagcacccgg tctgctggga
    10861 agcaagtctg tagacgactc catggctgag ttcgctctgg gtcaggcagc aatggtacag
    10921 aacggtaact ggggttggag ccagatcaat ggcgtagacg gtaacaccgt taaggctgag
    10981 gatgttaagt tccttcctat ctatacagga gttgaaggtg aagagaacca gggactgtgc
    11041 accggtactg agaacttctt ctgcatcaac aaggaagctt ctgctgagga tcaggcagca
    11101 tctatcgcat tcgttgaatg gctgttctct tctgaaaccg gtaaggcagc agtaaccaat
    11161 gaccttggct tcattgctcc tttcgatacc ttcagcgatg atgaaaagcc caccgatcct
    11221 ctggcacagg aagttcttcg ttacatggca gatacctcca agacatctgt atcctggaac
    11281 ttcacttcct tccccagcca gcagttcaag gatgacttcg gtgcagctct tctggagtat
    11341 gcttccggtt ccatcgactg ggacgctgta aagacaaaag tagttgacga atgggcagct
    11401 gaaaaggccg caaccgcagc taactaattg atctcagctt ataactattc tgaatcgtta
    11461 tccatcttat ataacttcag acacaggggg cggtggtggt accacggccc ccgtgtttgt
    11521 aaaacccgga atatcatgta aaaaggaatt aataggagaa ggcttatgca aaagacatta
    11581 aaacgatatt ttcccatttt tgtactgcct acattgatcg cattttcctt tgcatttatc
    11641 ataccgtttg ttatgggcgt atatctgtcc ttttgcaaat ttaaaacgat tacaaatgca
    11701 caatttgtag gcctggaaaa ttatatcaaa atttttgcgg acaaagattt tgtcaacgca
    11761 ttcggtttta ccttaaagtt ttctgttgta tctatcatta ccattaatgt atttgcgttc
    11821 attctggcac tggcgctgac cagaaagatc aaaggaacca acctgttccg taccgtattc
    11881 ttcatgccga acctgatcgg tggtatcatc ctgggttata tctggcagca gatgatcaat
    11941 gcggtattgt tgaaatatga gaccacactg gtagctaatc ctacctatgg tttctgggga
    12001 ttggttatcc tgatgaactg gcagatgatc ggttatatga tgatcattta tgtagcagga
    12061 ctgcagaatg tacctacaga cctgatcgaa gcagcacaga ttgacggagc tacttcgtta
    12121 cagactttat tcaaagtgaa aattccgatg gttatgccat ctattactat atgtttgttc
    12181 cttactgtat ccaacagttt caagctcttt gaccagaacc tggcgctgac tgccggtgct
    12241 cccagcaaaa agaccgcaat gctggctctg gatatttaca atactttcta cggcagaaac
    12301 ggttacgaag gtgtcggtca ggcaaaagca gtactgttct tcatcgttgt ggcagtcata
    12361 gccctgggac agctggtact taccagacgt aaggaggtag aacaataatg actaagaaaa
    12421 aaacttccaa tgtgatcata ttttgtatcc tgtgtctgct ggtagttgca tttttaacac
    12481 ctattttctt cgtgctgatc aactccttta agggaaaact gttcatcagc aacaatccgt
    12541 ttgcactgcc taccgcagag acctttgtgg gattgaccaa ctatgtgaac ggtattgaaa
    12601 aaaccggttt tttcacagct ttcgggtggt ctgcatttat cactgtattt tccgtggcag
    12661 cgatcattct gttcacttcc atgactgcgt actatctgac cagagtgaaa accggcgtga
    12721 ccaacgtgct gtattacatg ttcgtatttt ccatgatcgt gcctttccag atggtaatgt
    12781 tcactatgtc caagctggct aatatgaccc acctgaacaa ccctcccgga atggtgcttc
    12841 tgtatctggg atttggttcc ggtctgtccg tgttcatgtt ctgtggattc atcaaatcga
    12901 ttccgctgga catcgaagag gcagccatga tcgacggatg caaccctttg cagacattct
    12961 tcggtgtcgt aatgccgatc ctgaagccca cagccatcac agtagccatt ctgaatgcca
    13021 tgtggatctg gaacgactat ctgctgcctt atctggtgat cggcctgagt actaattaca
    13081 agaccatacc ggttgtagta caatacctgg taggttctta cggtgcaaag gatctgggtg
    13141 ccatgatggc actgctggta ctgtccgtaa tccctattat cgtcttctat ctgacctgtc
    13201 agaagtacat tatcgagggc gtggtagccg gtgcagttaa gggctaaaaa atagaaagat
    13261 gtggttatta atatgagaaa aagcggaata ttactgccgg tgagcagcct gccgtcccgc
    13321 tacggaatcg gttgcttttc caaggaagca tataaattta ttgacagatt aaaagaagcc
    13381 ggtcaggcct actggcagat tctgccatta ggacccacca gctatggaga ttctccgtac
    13441 cagtcttttt ccacttttgc gggtaatccc tactttattg atcccgagga tctggtggcg
    13501 aggggctatg tgaccgcaga gcagtgcaat acctatgact tcggcacgga tatccattcc
    13561 gtggattacg gcaaaatcta taagagccgt ttccgcctgt tgaaggaagc gtttgataac
    13621 agccatattg aagaagatgc gagatttcag gagtttgtac agaagaatgc atactggctg
    13681 gaggattatg cactttatat ggccatcaag gatggtttcc gcggcatctg ctgggcagcc
    13741 tgggaagagg atatcaagct aagaaccccg gctgccatgg acaaatatcg cagaaaatat
    13801 gcgaaagaga ttatgtttta ccagttccag cagtatctgt tccaggtaca gtgggaaaaa
    13861 ctaaagacct atgccaacga taacggcatc aagattattg gggatattcc catttatgtg
    13921 gcatttgaca gctcagatgc ctgggcgaat ccggagctgt tccagttcga tgagaattgt
    13981 gagccggtag cagtggcggg atgccctccc gatgcgtttt ccgtcaccgg acagctgtgg
    14041 ggcaatcctc tgtataactg ggaacatcac aaggagaccg gttatgcctg gtggatgcag
    14101 cggctggcag cctgctttga acgctatgat gtggtacgta tcgatcattt tcgtggattc
    14161 gatgcttatt atgcgattcc ctatggggaa cccactgcgg agcacggcca ctgggaacat
    14221 ggcccgggat atgatatttt ccgttccatg aaggaaaaac tgggagaaaa agaagttatt
    14281 gcagaagatc ttggcttttt aacaccatct gtgatagaat tagtgaagaa atccggttat
    14341 ccaggtatga aaattttgca gtttgcattt gattcccgtg aggaaagtga ttatctgccg
    14401 cataattata cacataattg cgtggtatat acgggaactc atgataatga tactgtgatg
    14461 gggtggtatg acaccttgaa gggagctgac aaaaagctct gtgacgacta tctgcgtctg
    14521 aaaaatgcgg acggagagcc gattcccaga gagcagcttc cctgggaatt tgtgcgtgcc
    14581 gcaatgtcca gcgtagcgga tctggcgatc attcccatgc aggattatct tggactggga
    14641 tcggaggccc gcatcaacat tccttccaca ctgggcatca actggaagtg gagaatgggg
    14701 aacggagatt ttacaaagga gcttgcagga cggatccgca gtatgacgaa gctgtacggc
    14761 agataggtcc gtaggcttgg aaaggtttgt gctccatgga ggaaatcacg atcaaagaca
    14821 tcgcaagaat ctgcggtgtc ggtgtcagca cggtatcccg tgccataaat aaccacccgg
    14881 acatcaatcc tgagaccaag gaaatgatca tgcgggtgat caaagagaat aactacgtac
    14941 caaacaacag tgccagaaac ctgaaacgtc aggatgccaa ggcaattgca gtactggtca
    15001 aaggtattaa caactctttc ttcagccaga tgatcaaggt attggaagaa gagatcaagg
    15061 aaaaatctta cgccatggta ttgcagcatg tagactatga cgaggacgaa gtggaagtgg
    15121 cactgaagct ggtcaaggaa aaaagacttc gcggcattat cttcctcggc ggttatctgg
    15181 tgcacagtga ggaaaagctg gcacagcttc atgtgccgtt tatcttaagt accacgggaa
    15241 tgctgcccaa gggcttcagc cgcaacacct attcctccgt agcggtggat gataccaagg
    15301 aaagctacaa gatggtggac tatctgtgca agaacggaca taaggatatt gcaatcctct
    15361 gtgcccgcaa aacggatgct tccatcggta agctgcgtct ggagggctat aaaaaggcgt
    15421 tgaaggataa cggaatagaa gtaagagaag aactgatcct gcctatgagg agtgatttgg
    15481 aagattactc cctggagaat ggctatatgg tgacgaagga attcctcgag aagcagattc
    15541 cgtgcacggc gatcttcgca atctccgata tgctggcgat cggtgccggg aaagccttgt
    15601 ctgatgccgg atacaaggtg ccggaggata tttctctggc aggctttgat ggtgtggaaa
    15661 tcgggaaata tgttatcccg tccttgacga ctttaaagca gccggtggac agcatggcga
    15721 gagagacggc ccggatcctg ttcgacatca tcgggaaaaa ggcgaagcac cagcactgcg
    15781 tgttcgacgg agaactggtg gtgcgggatt ccactatgcc gaggacttag actatgcaga
    15841 tacatggttt taacaagaca actttattag attatccgga acatattgca gccactgttt
    15901 ttacaggggg ctgcaatttt cgctgtccgt tttgtcacaa cggggaactg gtgctggatc
    15961 ccggctgcca gccgctcatt ccggaggaag aagtactctc ttacctgaaa aaaaggcagg
    16021 ggattctcca gggcgtctgt gtcaccggag gagagccgac tctgcagcgg gaccttcggg
    16081 cgttcctgca aaagatcaag gagctggagt atcccattaa gctggatacc aacgggtata
    16141 tgccgggggt attgtgggaa ttgttgcagg agcacctgat tgattatgtg gcgatggata
    16201 ttaaggcttc cagggataat tatgctcggg cggcgggact gccgcatata gatatctccc
    16261 acatcgagga gagtatcggc atcctgaaaa gcagtggcat cccctatgaa ttccgcacta
    16321 ccgtggtaaa gggaatccac agggtggaag aattcgagga catcggtaaa tggatcgccg
    16381 gatgccctgc gtattatctt cagaattacg aagagaatga aaactgcctg taccggatga
    16441 tacagacagc ggaatgtgac gatgtgaaac cgggcggtgt agatccgttc ggcgcatttt
    16501 ccaaagagga actggaacag atggaggaac tggcccagaa atatgtgggg aaacttgtgc
    16561 tgcggggcgt ggaatagacg ctgtgcctcg cagctacata agctgttgta tttttacaaa
    16621 ctcatcaaag cgaggtaagg ccagtgtgac tttcccgtat tctttggaaa taatgacacc
    16681 ttttctcttt aaacgctcac gatagacaga gaaaagttca gatttcatat ctaatcgctc
    16741 acggagagct tttacctgag tctctccgct gagagacatt tcagttacga tttttttatc
    16801 cagatcggat aattccgacc atattttact gtatacatat tcatccagat attggtcata
    16861 ttccggcaaa atctgttcca aagtcttttt atcgcgattt tcccagtaga gataaccaag
    16921 tacctgaaag gcaaaaggat agcctttggt cagtgaagcc agttctccgg cagcatctat
    16981 atccagagaa aagatatcca tatattgttt gcggatagcg gtataattga gtggctccag
    17041 gattaatttg ggtgcacggt ataaaaaggt cagtacttta tcattttgaa gattataaat
    17101 attttcatat aatcccgtca tcagtaaaaa gatgggatag tcgtttcgta aaaaaatctg
    17161 aaaggagctt gcaaagattc ggatatattt ggaattcgtc acttcatcaa cgctgatcag
    17221 caaccttttt tcacttttct ggatatattt caacatgagt tccagagcgt tttcgatatc
    17281 cgtgaccgga gcagtgtttt caatggatac tcccagacca aaagcagaaa agtcaagttt
    17341 tgcattaata aacagcgtgt gtaattcggg aatgctgtag atttttgcag caagactttg
    17401 cagtaaatct ctggtaggat tcagttccac aacgatccag tcttcgcttt ttcgtaattc
    17461 cgaagcaata tttgtcatca ttacagtctt gccagagcca cggacaccgg tgatcatgta
    17521 gatctgattg gatggaatct cggcacaaaa gctttccaga atttggtttg tttccgtgag
    17581 acgggagata tattgcaacg gtttttttcc aaaagataac gtaaacggat tggcactcat
    17641 aatgaaaacc tccttttctt atataagttt atataacttt taaaatctta tataacttta
    17701 tataagtgta tataactttg taattattat ataagtttat ataacttttg gcaagagata
    17761 ttgcggctat gggaaatgaa tgcagaagga aaatgtaaac gcttactctt cctaaataga
    17821 catttcatga gaaaatgtgc atattttata aaaaataaat gttgcgcaaa aatattagct
    17881 aaaaattact caaaaaatat atgaataact tccaaaaata ttcaaaatac acaaaaatgg
    17941 tttactaatg tgaagcgata gtgctaatat atagtacata gtgttgtaag agcttacaaa
    18001 tataatcgtt tataacacat gctaagtgga ggtatgggaa tgttaggcag gagaggaaga
    18061 aaagagagaa aacgactgat ggcattgctt cttgcgggag ccatggtgct gtcgggaatg
    18121 ggaatcagcc ccatctctgt acaggcagag gaaacggaac ctgtggtaga acaggtaagt
    18181 gagacggttt ctgacggaaa tgcggaaaga gccagagaag tgttgggaga ggaagaaggt
    18241 attgcggcaa cttcgacaac acaggatgcg aatgcagcaa tggctgctgc gcagagtgga
    18301 acggaaaaag acctgtatga attggatctg agcaagggtc tgacagcagg aacaacgtat
    18361 gatggcggaa tttctgtact tgaaactatg agttttaaat ctggttcagc tagtttagat
    18421 ggggtaaagt atacaggatg tgtacaggga gataattcgc ctaacccgaa caaaggaaaa
    18481 attcctacca gtggtgctgc agtaagactg gaggcggaaa aagatggcag aatcaaagtt
    18541 gtatttaaag caaaaggcaa agatatttat tttttgcagg atgtagatgg caagccccaa
    18601 gggattgaga cctttaaggc gggagcagac ctgattgcca aaacttatga ggtggaaaaa
    18661 gggggcactt attatttata tgcaagtggt agtaagatta tgatctatgc tctgaccgta
    18721 gattacagat cttccgtaag ctgggatgac atagcagctc ccgttctggg aacacctttt
    18781 gtcaaggacg gaaccatcac cgttccgtac actgcacaga tcggtggaat ctatgcaaaa
    18841 tctttggaag tgacgatgta tgatgctgat ggtaatgcgg tggagacaaa atccgttact
    18901 gcacagggaa cctctggaaa tgcagagttc cagcctacag cgtccggcac ttacagcttt
    18961 gaagcaaaac tgataggaga aggcgagaat acaaaagcaa gtaataagac aacatcaata
    19021 gaatttgtat tacctatggc aacgccgaag ggcttaaatg ctgagaataa gggacacggc
    19081 aatgtgaaac tgacctggca ggcagttgcc gaagcagaga gctacaaggt atctgtggac
    19141 ggcacggttg ttgaagaagg agtgactgca ttggctcaga cgatcaaagg attgacggta
    19201 ggtcaggaat atacatttca ggtagctgca gtcagaggag aaaatgtatc cactccggcg
    19261 gaagtaaaac tgacaatcac tgaagatgca gtgaagacct ggaattttgc agcatttggt
    19321 cagggcgttg acaacaaaac agcgaataac ggttatagtg gaagcctgga tacaggtgat
    19381 ttgaagatct gggagaagaa cagcaaagga aagctggtag cagcttcaac ggatggtctg
    19441 gcattctact acaccacgat tgatccggca acagagaact ttacgttatc tgcagatatt
    19501 acggtggacg aatggacata caccaacgga caggaaggct ttggccttat ggcagctgac
    19561 cgtgtgggtg agaatggaga ttccggtatt ttctggaaca attcctatat gctgagtgct
    19621 acaaaggtgg aatatctctg ggatgccaca gaaggcaagg tatctgatgc cggagacaaa
    19681 tacaccatga aacttggtat cggttcccag gaaaaaatag gtgtaacacc ggaaaatatg
    19741 aacgacagtc ctacagatca gggcttccgg tctaccatga ctactctgga gaccagtgct
    19801 gcaaacagcg gaaacggcgc gggaacttat aacatagtag gcaaatatac caattctgca
    19861 gaagctgtga aggatattac ggatctgaac gtgcagacca ctttccacat gattttgcag
    19921 agaaacaata ccggttattt cctgagctat accaatgtgg aaaccggtga gaccgtaacc
    19981 aataaatact atcacggcaa agacggagac gaactgacca gattggatgc agagaacatc
    20041 tatgtaggtt tcttcgcttc cagaactgca accatcagcg ttgccaatgc agtgctgaca
    20101 acaatcgatc cggccaaaga tgctccggca gaatccagac cggtcactta tgtaacaccg
    20161 aattatacga ttgattctgc ttctgtagcc aacagtgcgg attacgaact ggtatattac
    20221 ggcaatgcgg acggaacatt gaaaattacc aacagtgaca atgaaaccgt attgtcagat
    20281 gcgggtgtaa aggcaaatac caaggtatat acccatacga cactgaaaag cggaagcaac
    20341 gttttccatg tagagtttac cccgaatgcg gattacagac cttcagagta ttctctgttg
    20401 agtagctatg atacagtaac atttgacttt acagtaaatt ataatgtaaa gggtagaacg
    20461 gttatttatg ctgctcctga gggaagaagt acgggaagcg gtaccaacag tgatcctgtg
    20521 gatatttata ccgcagtgaa gagcgtggct ccgggccaga tgatcgtgtt gaagggcggt
    20581 acttatgcac tggataagac ggtgacgata gacagaggcg tcagtggaac tacagatgcc
    20641 agaatctata tgattgccga tccggacagc acttctcgtc cggtactgaa tttccagaga
    20701 aaatgtgcag gtatgatcct ggcaggtgat tactggtatt tccagggatt tgatgtaaca
    20761 ggttctgcta attccctcaa gggtattcag gtatccggca gtcacaatac actggatgat
    20821 attatggcat atagaaatgg caatactggt attcagatca gccgttacaa gagcacagac
    20881 ggaagaagcg actggccttc ctataacctg atcctgaact gtacttcata tctgaacgca
    20941 gatgcgggat atgaggatgc agacggattt gcagctaagc tgacgattgg tgagggtaat
    21001 gtatttgatg gatgtattgc agcatacaat gccgatgacg gttgggatct gttcgccaag
    21061 atagagaccg gagctatcgg tcaggtggtc attcagaact gcgtggcatt caagaacggt
    21121 tatgtactgg atgagaacgg acaggaagta gatgccggta acggtaacgg ttttaagatg
    21181 ggtggttcca gcatcagcgg acatcatatt ttgcgcaaca gtgtatcatt cggcaataag
    21241 gcaaagggta ttgattccaa cagctgtccg gatattgagg catattcttc taccagttat
    21301 aacaatgagt cctttaacgt ggcattctat acaaatgacg caaagaatac tgcatttatg
    21361 gcaaaaggaa tcctgtcctt taaggatagc agcaatgcag cagggcagac ggttacagaa
    21421 cagttcaaac cgaaaggaac tcaggagacc agtgcctacg aaaatgcaat gaactattat
    21481 tggagaggcg aaaactctac caacagtgaa ggaatagcag ctacagcaga atggttccag
    21541 aacatggata tgaacagtgc aatccacgga ggcattcgca gaaatacgga cggtaccatc
    21601 aatatgaacg gtttcctggc agtgacttcc gcagtaccgg aaggtgttgg agccagaatg
    21661 accggaaccc cttctgctgt atttacggta gcggcagatg tggtgaacaa cgatgatgac
    21721 gatgacgatg atgatgacaa cagcggttcc tctgcagcac ctgcagtaga ctggacggat
    21781 gtaagcaact ctgtacagga taaggtggca gagatgatga aaaatcctgc tattgcaagt
    21841 gtcaacatga actttgtatg cagcggtgaa gtcaaggtgc ctcagaatgt actgaatacc
    21901 attaaaggta cgaagctgac ggtggcattc cacagcggaa acggtgtggc actgagcatc
    21961 agtggacagg atctgaagaa taaagatctg tcaaagattc agaatattga tctgacagta
    22021 gaccagacga gcaacacaat cccggcaaat gtggtatccg caaagtccgg aaccgtgaac
    22081 agacagctgg gtatcagaga taccggcagc ttcggagtga atgtcaacat tcatgtaaat
    22141 gtaggcaagg acaattccgg caagagtgca aacctgtatc gctataatac agagaaggga
    22201 cgcctggagt attgcggatc ctttaccata acttccaccg gacagtccat gttcgcattg
    22261 aagcgcggcg gcaattatct ggtaactgtt actgaccgca gaccttccga gagcatctgg
    22321 tatacagagg gcggttatac ggtaaagagt ggtgatacgc ttttcaagat cgctaagaga
    22381 aatcacatga ctctggcaca gttgcttcgc agaaatgtac agattacaaa ccagaatgtg
    22441 atcagagttg gtcagaaact gaatctggaa tgatcgtaaa aactgtaaga gatagagaaa
    22501 aaggacaaga cgggaaactg aaaaacgaga aaaacggatg gcttgggagg gccatccgtt
    22561 tttttgtgta tatgatgagt cagcctactc gattggaaat attttattgc acctgattgg
    22621 ggctcttctt tccactttcg aaatccagag cattctgcaa ggtagtctct gcaatggctt
    22681 ccagtgcttc ttttgtgaaa aatccctgat gggatgtgat catgacattg gggaaggaca
    22741 aaagccttgc ggtggtagaa tgctccagaa tctcatcgga acgatcctcg aagacgtttt
    22801 tcgtctcttc ttcatagaca tccagaccga ccccggcgaa cttgtgcagg cggatgccct
    22861 cgataagatc ctcggtcttg accagagcac cccgggaagt gtttaccaga atgacgccat
    22921 ccttcatttt cttgatggtg tcaagctgga tcaggtggta ggtggaatcc atcagcgggc
    22981 agtgaaggga gatcagatca ctttgcgcca gtaattcgtc taaggtcaca taggtgacga
    23041 aatctttcag atccgggttc tcgtagacat cgtaggcgat gactttcatg ccgaaaccgt
    23101 tacagatccg gcacatagct gcaccgatcc ggccggttcc gatgattccg gcaatcttac
    23161 catagaaatt gaagcccatg agtcctgcga ggctgaaatc attttcccgg accttgttat
    23221 aggctttgta taagcggcgg ttgcaggcca gagccagagc catggcatgc tctgcgacag
    23281 cttccggaga ataaccgggg acacgcatga cacatatgtc atatgcagct gccttttcca
    23341 gatccacgtt gttgtatcct gcgcaacgca gcaggaccag gcggacacct ttttcgtgga
    23401 gaatttttaa tgtggcttcg ctcacattgg agctgacgaa agcacagacg gcatcgaagc
    23461 cgtcagccag agcagcagta cggggatcca gatccgaccg gatgtaatca atctggatgc
    23521 cgggaaattt tttcagagtt gcctcgaaag attctttatc atagcttttg gtatcataaa
    23581 ataatatttt cataacagtc ctcctcgggt tgatttttat agtatgtgta ttcttatttt
    23641 taatcatact tagatttggc tttactatag ctcaaaatat gggaattgtc tatcatattt
    23701 aaaaagtgaa atcgagaaaa attataaaaa tgatgcagat atgatgcaat cggcagatat
    23761 actggcatca ggaggcgagg attatgaaaa aaatattttt tgcagcagta tttgcgggag
    23821 cactgttagg ttttacatcc tgcggaaacc gggagatggt gacggatccg gagatggaga
    23881 cagttataga cagcatcaag gagacacaga gcggtcagac gacggaagag acgatggccg
    23941 ggactgagga gactgcggaa gtgtccacac agacggaacc gacagaaggc cagagtgaaa
    24001 tacagggaag tgaatgggtg cagtttacca cagaggatca cgagggtagc agtgatcccg
    24061 aagacaagac cagaagttat acctattact atccaacagt tacagtgacg attccgggca
    24121 atgagacggt gcagatgaag attcagaaag agctggatga atatgtagac agttttgtag
    24181 aaggaatcga gaacggggag tttggtacac tttatgagga tatgccggtg tatgagcaga
    24241 gctatgagga tcttactttc cgtgtgatcc gcgcggatga taaggtgatc agcctgtcgt
    24301 gggggctgga agggtatgat cagggggctc atggatggta tactttgtac tatatgaact
    24361 attatacgca gaccggagag agaattactt ttgacagtct ggggagcgga ttccgcgaca
    24421 aagcactgga actggtgaca gcaaaggcag cggaaatgca ggcaaaggag gactgcttct
    24481 ttaacgatta cgagaagagt atcaaactgg tggtgttgga tggtacggaa gatttgaatg
    24541 ctatctatca ggaaatttat gggccggaca ttgcaggtac ggataatggc cccgcagacc
    24601 ctacattcta cattacagag gatggctttg tatttgaatc cggccagtat gtattacagt
    24661 cctatgcagt tggaattgtt gactttgaga ttccggcagc agacttcggg gatgatctca
    24721 cagcagatat tttttaatgg agtacaggat gctggcgggt atatttgcaa aatataggac
    24781 tttacatggg cgggcaccgt gtatataata agaacatcgg gaatgaggtt ctcccggaag
    24841 cacaggcttc gaaccgccgg aatttgaaca tatgaattcg atttggctga tgacttctgc
    24901 agaatatgca ggggacacca gcctttttgt tagaattcaa aatgtcctgc tgcgagaaaa
    24961 cggaggaatt tttatactta catcactggg actgatcttt ttactgggat tactcatggc
    25021 ggcggtctgc cagaggattc acctgccccg tattatcgga atgctgctta cgggtattat
    25081 tctggggcct tatgtgttgg atctgctgga tgtgtcaatc ctgaatatct catcggaact
    25141 gcggcagatc gcgctgatca tcattttatt aaaggccgga ttatcattgg atctgaagga
    25201 tctgaaaaaa gtgggccgct ccgcagtcat gctggcttgt gttcctgcca cttgcgaact
    25261 gttggctttt gtgatatttg cacccatgat ccttaacatc agcagagtgg atgcagcggt
    25321 catgggagca gttctggcag cagtgtcacc tgcggttgtg gtgccgcgga tggtacatct
    25381 tatggaaagt ggctatggta cggagaaaag cattccgcag atgattctgg ctggggcatc
    25441 ctgtgatgac atctatgtca tagtattgtt ttccactttt gttggaatgg cacagggtgg
    25501 acaggtcaat gccctggatt ttgtgaatat cccggtatcg atcctgacgg gtgtgttgtt
    25561 cggtgcattg gtgggttggc tgctggggca gctttttgaa ctgttgtacc gaaagggcag
    25621 ttatgtgcgc aacagcatga aagttattat catcatgggt ttgtccttct tattaatggc
    25681 tttggaaacg ctgttaaagg gaaccgtggc cttttccggc ctgttggcag tagtgagcat
    25741 ggcctgcgca ttgaaaatgc gggcaccgaa agaagtgacc ggcagattgt ctgagaagtt
    25801 cggcaagctg tggctggcag cggaggtagt gctgttcgtg ctggtgggcg cggcagtgga
    25861 tatccgctat actatggaag ctggactggc agccatagga atgatcctgt tggcattgat
    25921 attccgctcc attggtgtga cactgtgcct gttaggaacg aatctgaata agaaggagcg
    25981 tctgttctgc atgatcgctt atttaccgaa ggctacggtg caggccgcca tcggttccgt
    26041 gcccctggcc atgggattgc cctgcggcaa gatcgttctg tccgtggctg tgctggccat
    26101 ccttatcaca gccccgctgg gagcactggg aatggatgcc acataccgga agctgcttca
    26161 gaaaaatgga gaaaataata tcatttgaca gccatccggc acgtgatcga gtcccggaag
    26221 gaagccgcaa aaacgtccac aacatctaca ccataagaac cgcaggcacg ctctcgctct
    26281 ggcgcagaac gtagcggtac ataattgacc ccaatacggt caataagtac cgacgtctgc
    26341 ttacggcctg cttgtcctta tggtgtagat gttgtagcgc aaactacgaa tatccaatgc
    26401 aaaaccccgg cagctacaac aactgccggg gttttaagga ggatagaaat cgttttaaac
    26461 tatgtacatc aggtttatta acccatggta ataactacgt tgtactcttc cttgcccttt
    26521 tcgtaaggaa tgatgtgtcc gtctactgcc ttgccgtcca cggtcatgga tacgacaccc
    26581 ttttccacgt tctgagggtt cttcacctga atgtgataga tgccctcacg gaacttacgg
    26641 gtgatggaga aatcaccgaa gcccttggga atacaaggat ctacggacaa tccctgatga
    26701 gtgggatata cacccagtat gtattgggag atatttacga aagtccatgc agcagtaccg
    26761 gtcagccagc tgttctttgc ctcgccgtag aattttgcat ccttaccggc taccatctgg
    26821 gaatatacat aaggctcggt acgatggatc tcactgatat cctcaatgta tgcgggacag
    26881 gtcttacggt agatctcgaa agctctgtca cctctgccca gaacggtctc tgcgatggat
    26941 acccagggat tgttgtggca gaagataccg gcattctcct tatatccggg gggataagag
    27001 gatacttcac caagttcgag gtgatactcc gtgtatgcag gttgtaagat catgacacca
    27061 tacttggtat ccagtctctc ttttacagaa ttcaatgcct tttcggcaag tccttccttc
    27121 acaccgacac cggccagtac gcagaagccc tgaggctcga tgtagatctg accttcgcgg
    27181 cattccctgg aacctacttt gtggctgtaa gcatcgtaag cacgtacaaa ccaatcgccg
    27241 tcccaaccat ccttcaggat cgcatcgtac attgcctgta cttccttacg tgcgcgggct
    27301 gcttcggtat cgtcacccat cagctcgcac agctgagcat attcttcgcc gtattttacg
    27361 aacataccgg caatgaatac ggattctgca acgggtcctt cactgggacc gaaggtctgg
    27421 aaagattctc cgggatgagc ggagaagcag ttcaggttca gacagtcatt ccagtcggca
    27481 cggccgatca gaggcagatt gtgagggcct ttgtggttga tgatatagtc taaggagcgt
    27541 tttaagtgat ccattagagg agcagctacg ctcatatcgt tgtcaaaagg aaccatctgg
    27601 gtcaggatgg aggtatcgcc ggtctctctt acataagcac tggtacctgc aatgagccac
    27661 agaggatcat cattgaagcc actgccgata tcggagttgc ctttcttggt cagaggctgg
    27721 tactgatggt aagcactgcc gtcctggaac tgggtggaag cgatatcaat gatacgctct
    27781 cttgcacggt ccggaatcag atgtacgaaa ccgagcagat cctgacagga atcacggaaa
    27841 cccataccac ggccgattcc ggattcgtaa taggaagcgg aacgggacat gttgaaagta
    27901 accatacact gatactggtt ccaggtgttg accatacggt tgactttctc gtcagcggaa
    27961 tctaccgtgt agcgtgccag cagctgtttc cagtaagcct gtaatgctgc aaaagcctca
    28021 tctacctgtg catcggtctg gtattttgcc agcatttctt tggcacgggt cttgttgatc
    28081 acaccgtagg attcccattt ctcgtcttcg gggttctcga tgtagcccag aacgaagata
    28141 aaggtcttgg tctcaccggc tgccagatcc acgttgatct gatgggaagc gatgggagac
    28201 cagccgcttg ccatggaatt cgtgcacttg ccgttctcta cagcttcggg agaatcagcc
    28261 cccctgtaag cacccaggaa agcatctctg ctggtatcga aaccgtcaat cttcgtattt
    28321 acggaataga tcgcataatg attacgacgc tcacggtact cggtcttgtg ataaatggtg
    28381 gaatcctgta cctcaacctc accggtgctt aagttacgct ggaaattctt catatcatcg
    28441 tcagcgttcc acagacacca ctctacataa gagaatacgg acagcttctt gtcctcggag
    28501 ctaccgttgg tcagggtcag ctgggagatc tcgcaattat cgttcagagg aacgaaggag
    28561 agcacggaag ccttgacacc gttcttggaa gaggtgaagc ggctgtaacc gataccgtgg
    28621 cggcactcgt aggagtccag ttcagtcttc gtaggctgcc agccggggtt ccatacggta
    28681 tctccgtcct taatgtacag atatttaccg ttgatgtcgg ccggtacgtt attataacga
    28741 taacgggtca gacgcagcag ctttgcatct ttatagaaag tatatccgcc gcaggtgttg
    28801 gatatcagac tgaaaaactc attgcagccc aggtagttga tccagggaag cggagtcttg
    28861 ggagtggtga tgacatattc ctgatgcaca tcatcaaaat gaccatactt cataagaaaa
    28921 tctccttctt tcatgttgtt gtatgttgag tgtttgtttt ttcgctgcgg agtgcttaat
    28981 gttaagtaaa cgattaagtg ctccggattt ctgatttgta ttatacagaa gcaaagggaa
    29041 aaatgcaata cgaatcttga aaaagcccgt aaaatgcgca atgtatacaa aaacagtggg
    29101 gaaacatggg cagaagatac aaaatgaaag aaccttcgaa acaccggttt tacgaaacaa
    29161 aataatagca aacgttatcg cacactttgc ctttatatag aagaaacaca atctatgtac
    29221 aagccaaatg tacaaaacag cctatctgaa attgttaatt atagacaaag tggatacttt
    29281 tttgaaaaaa actgcaaaaa agtattgcaa aatgctcaaa gatgttatat attaaaacta
    29341 cgaaaacgat taagttacaa aacaggtcgg acggaatgac gaagacatgg aacaggagaa
    29401 agatatggca tcactgaaag acatttccaa gatttgtgga gtctccgttg caactgtcag
    29461 caaggcactg aacgaccaca aggacattgg agaagaaaca aaagcaacta tcaaaagggt
    29521 cgcaaaggaa atgggatatt ccccgaacct ttccgcaaga gctttgaaga cgaacaggac
    29581 ctacggcatc ggagtcctct ttgcagatga agccagaagc ggtcttaccc acgattactt
    29641 ttccagtgtg ctggacagtt tcaagaggac ggcagagaag agcggttacg acattacatt
    29701 cattaatagc tgcaagaacc ggccggatcg gatgtcctac ctggaacaca gtaggtacag
    29761 aggctttgac ggagtggtgc tcgcctgtat tgatttcgga gatcccgaag tcatcgaact
    29821 ggtacagagt tccatcccgg tagtcaccat tgactacctg ttcaacaatc gtatcgcaat
    29881 catgtcggac aatgttgcgg gaatgaaaga actgctggag tacatatata gtagaggaca
    29941 taggaaaatt gcttatattc acggagcaat gtcagcggtt acctcgggac ggttgtccag
    30001 cttctaccgg acggcggaac gtctgggact ggagattccg gacgagtata tcagagaagc
    30061 agcttacaga gacacaacaa caacctatcg tgtgaccaat gaactgctgg atctcccgaa
    30121 tccaccgaca tgcatcatct atccggatga ctttgcttct ttcggaggca tcaacgctat
    30181 cagacagaga ggattacaga tccctgaaga tatctcggta gcaggctatg atggcataca
    30241 ggcagcaagg cagttggagc ccagactgac cacagtccaa caggacacag aacagatcgg
    30301 ctgcctggca gccagagaac tgatcagtct cattgagaga cccaagacca ccatcataga
    30361 acaggtcatc gtacccggga aagttgctga aggcaagtcg gtggccagaa ttccgacaga
    30421 caattaatca ggcatttata tggaagcgac gcaaccgccg acatatgaat ataaatgcaa
    30481 caaacactta caacaaacat tcaacaaaaa atgggaggaa acaaacatga agaaaaaagt
    30541 acttagtgct ttacttacaa ctgctatgct tgccagcatg ctggtaggtt gtggcagcag
    30601 caatgaagct cctgcagcat ccaccgatgc ggcacccgca gcttccaccg aagctaagac
    30661 cgaggcgcct gcatccaccg aggcagcaga gcctgcagca gcagaggaag gcaaagtctt
    30721 caacatctac tgctggaacg aggagttcaa gagccgtctg acagatcatt atcccggata
    30781 tgaagaagta gacggaacca ccggtaagat cggcgacatc actgtaaagt ggaacatcac
    30841 tccttctgat gacaacgctt atcagaacaa tctggatgaa acactgttaa agcaggctga
    30901 tgcagcagct gatgacaaga tcgacctgtt cctgattgag gctgactacg cattgaaata
    30961 tgttgatacc gactacacaa tgcctgttaa ggatctgggc atcaccgatg cagatctggc
    31021 taaccagtat cagtacacca aggatgttgt taccgactcc aacggcaatc tgaagggtgt
    31081 ttcatggcag ggctgccccg gcgttctgat ttacaaccgt gaggctgcta aggctgttct
    31141 gggaaccgat gatcctgcag aggttcagaa gtctgtatct gattgggata ccttcaccgc
    31201 aaccgctgag aagatgaaag cagccggtta caacatggta tcttccgtaa acgatactta
    31261 tcgtgtatac tccaacaacg tatccagcaa atgggtacag gatggcaaga tcgtaatcga
    31321 tgacaacctg atgaagtggg ttgatgactc caagaagatg gttgacgcta aggagaccgg
    31381 taccttcgac ctgtggtctg atgactggag caaaggcttc tatcccgatg gcaaagtatt
    31441 ctgctacttt ggacccgctt ggctgatcaa cttctccatg gcagctgata ccgatggatc
    31501 tatcgcaaat cagggtggct ggggcgctac tgaaggacct cagggctttt tctggggcgg
    31561 tacttggatc tgcggtgcta ccggaaccga caacgcttct ctggttaagg atatcatcct
    31621 gaagatgacc actgatgaag acatcatgaa agagatcgtt gtagctgatg atgatttcgt
    31681 aaacaacaaa cccgctatgg aagctatggc agcagatacc tcttatcaga gcaaggtatt
    31741 gggcggacag aaccctctgg ctatgttctg cgcaggcgct gagaagatcg atctgagcaa
    31801 cctgtctgca tacgatcagg gatgtaacga agagttccag catgctatga agaactactt
    31861 cgatggcaag gcttctctgg atgatgcact ggatctgttc tacaagggtg tagaagagaa
    31921 gtatcctgag cttactcact aatccgaaat gataggcgaa tagcgaaaca ataaggaatt
    31981 gagaaccgtg cctgcctggc tggtcaggta ggcacggttt ttctctcaaa attcccggtt
    32041 ggcagaagct tttttcagaa gaagataatt aagaacaaaa agaagagagc tgctgcgagc
    32101 ctgatcggaa acaaagaaag gcacgaaaaa cgtaaagtcg ctaagagagc atgacggcaa
    32161 aagggaggaa tcattatggc aaaggtaaaa cgcggaaagg tggtacgcta taataagtgg
    32221 ggttatattt tcctgatccc ctttgtagta gtgtatgtat tattccagtt gatcccgtta
    32281 ttcaggacat tttattacag cttttttgaa aattatcgtt ccggactgac acagatcgga
    32341 ccgaatttca tcggatttgg taactatgca aagctgttgt ccaatccgga cctgtggatg
    32401 tacaccaaga atacattgat catgtgggta ctgggatttg taccgcagat catcatatct
    32461 ctgcttctgg gtgcatggtt ctcggatccc agccttagat taaagggaca gagattcttc
    32521 aagacagtta tctatctgcc gaacctgatc atggcttccg catttgcaat gttgttcttc
    32581 acgctgttct ctgatggtgg tcctatcaat gaaatgctga tgcagatcgg aatcttaaaa
    32641 gagccatatc agttccttac aaacatatgg agtaccagag gtctggtagc attgatgaac
    32701 ttcctgatgt ggtttggtaa tacaacgatc ctcctgatgg caggtatgat gggtatcgat
    32761 acttccctct tcgaggcagc cgaggtagac ggagcaacct ctacccaggt attcttcaag
    32821 attacattac ctttgttgaa gccgattctg atctacgtaa tgatcacatc cctgatcggt
    32881 ggtctgcaga tgttcgatgt acctcagatt ctgaccaacg gtaagggcga tcctatgaga
    32941 tccagcatga ccctgatcat gtacctgaac aaccatttgt acagtaagaa ctacggaatg
    33001 agtggtgcgt tgtctgtaat gttgttcatt atcacaggta tcctgagctt gatcgtattt
    33061 aagttctcga acggcaaaca ggataagtag gaggtggcag aaatgttgag cgtagaaaca
    33121 aacgagacaa aaaaaggtct gggcattcat gccagaagag cacttgcata tatcgtactg
    33181 gttattatga gttttctctg cctgttctgg ttctatatac tgtttgtaaa tgcaaccaga
    33241 tccaaaggcg agctgacaca gggatttacc ctgctgccca gcacacatct gatccagaac
    33301 tggaagggtg tcatggcagg aaccctgccg gtagtcagag gtatgatcaa cagccttgta
    33361 gtagcaacct gcagtgctgt gctgtgtacc tacttctcta ccatgaccgc atatgcgatc
    33421 catgtatatg attttaagtt aaagaagttc atgtttacct ttatcctggc aatcatgacc
    33481 attcctaccc aggttaccgc actcggattt atccagttgg tagctgatat gaagctggag
    33541 gataacttta tccccctgat catcccggca atcgcagcac ctgctacttt cttctatatg
    33601 aagcagtaca tggaaagtgc attgcccctg tctctggtag aggcagccag aatcgatggt
    33661 tccggtgagt tccgtacatt taattccatc gtattgccgc tgatgaagcc ggcaatcgca
    33721 gtacaggcga tcttcacttt cgtagcgaac tggaataact acttcacacc ggcactggtt
    33781 ctgcatgatg acaagatgaa gacattacct atcctgatcg ctcagctgcg ttccgcagac
    33841 ttcctgaagt ttgatatggg tcaggtgtat gtaacgattg cattctctat cttccctgtt
    33901 atcatcgtat acctgttcct ttccaagtac attgtccagg gcgtggcaat gggaagcgtc
    33961 aagggttaat atttcgccag caacattcaa tacataaatc tctcctgaaa agcggcactc
    34021 ttccccggag tggcgctttt cgcttgcaaa aaaacgggga aacgggtata aaataaagag
    34081 aagggtgaac gtgtattttg aggaagaaag aggtaattgc catgcagatg tatgaggtga
    34141 cagcactggc accggaagga ccggaggaag tctatcaggc gatggtgttt gcagaagatg
    34201 aagatgatgc cttgaaccag ctggaggaac agctgaagga gcagggtatt gcccatggaa
    34261 tgtgtatggc ggaagaagtg tagtgtaaag tgtgaaaaga acttctaatc actcagagca
    34321 aaggctcttt cgcgaagaag ttctatgttt cacacgcaaa tttgcgccaa tgcgcaaact
    34381 ttgcaggtta tggaaggatg tgagtgttgc aaatggaaaa ttcctatcgt ttttttgaga
    34441 ataaagattg taaatatttc ccctg
//
LOCUS       DAWEJW010000017        33280 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_572_length_33280_cov_55.744861, whole genome shotgun sequence.
ACCESSION   DAWEJW010000017 DAWEJW010000000
VERSION     DAWEJW010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 33280)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 33280)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..33280
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_572_length_33280_cov_55.744861"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    976..985
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8361 a         8002 c         5945 g        10962 t
ORIGIN      
        1 ccaatgcggt tactgctaat attcctgctc cgatcagagc taataatttg ctgtgtctta
       61 ctctcataat aatgtcctcc atgtgtttgt ttttctttct cttgattacg caatcatact
      121 aacatggagg aaattcacat aatatcacag tccggtaatt tatttgtaag aattaggtaa
      181 aatcacatgc tctctaagat tccgtcggca atggcccggg cgatctcgtc aaaccggctg
      241 tcaaagagtt cgttgtccgc atccgtattg ataaatccca cttctaccag caccgccggc
      301 atatttgtgc tgtttaacac caccagattc ggtctttcat tcactcccag attcacgaat
      361 cccacctgca ccaatccggc attgatgttg tacgccattc tggctgccgc tccatagcgg
      421 ttgtagacta agctctccac tccgctgtac tggttcggat aaggactgga attgcgatgg
      481 atcgagacaa aataatctcc tcccacctca tttccctcct gcgccttctg ataaggagac
      541 tcgtagatat ctgtggtccg ggtgtaatac acatccactc cgttttcttc taagatgctt
      601 cccaccgcca gtgtcagcgc tagcgcatcc tcactttctt ttcttccatt ataagtggct
      661 ccggggttcg caccaccgtg attggaaata cggctactat attctatatt aacaaataag
      721 cagacaacag gtttattaat tgccctgtcg tctgcttatt tgttgcctat gatatgacat
      781 catttttcta tgagtacatg ataaaattgt ttttttctgg tatttttaat tttcagcatc
      841 ccggatacac atttcatgct atccacatat gccggcgtta caaatatttg ccgaggattc
      901 tttcggatta ttctattaac tattgtttta tgtgtataaa tctcaataat cgttccttct
      961 ggaatacttt catatnnnnn nnnnnttcat tcttaaactg tttaagtcta ttcgctttag
     1021 ctaccttttg aatccaccaa ttattattca taaaaatttg cacgtaattc tttttccaac
     1081 acgggcagta ttttatgaga tatctacttt tattacccct atttgctttt gctataccta
     1141 atactatata taaaatccat attgcaaata tcacaattcc tattatctct tccacattac
     1201 atgcaagtat taaaacaata aatgcaccga ttatgatatc ttttggtaca atataaaata
     1261 tttttttcat tatgtaactc ctcctgttga taattattta ttctcttaaa ggagctgcat
     1321 tcagctggta aaatgcgctc ttttaccagc tatttactct ttacaggttc cggtccaggt
     1381 ccgacccaga tgcggaatgc tttcttacca taatctctgg cataaatctt tgtgccatcc
     1441 tttgtggtaa tataagtacg aaaaatgtac ataatatatc cactcctttc tgcaagttgc
     1501 tcttgcaaaa agagtgccac tgtgataaat tataggtgtc tagattataa tttataccag
     1561 ctgagcagta gcaatacttt tcagcaagag ccaagcatct cgtgtgcttg gttttttata
     1621 tgtaatagct tctttgaagc ttatacatac ctaattatcc taactaagca atagaaaatt
     1681 gagaaagtat ttttctttca tatccagtat attctctttg accatattgc aaccaactat
     1741 tatagtattt atacgcataa caagcggctg cataactaat gtcaaatttt ttcataatgg
     1801 aaaaaggact tatatcttca accatattat gtataagtgg aggtggcgcc aacgcatact
     1861 ttgcaaaaaa ctctgcttct gcctcctctt catctccttc ttttatatgt cctaaagcat
     1921 agtgtcccac ttcatgcatt atagtatgat ttatcctgcc ataactttga caatgatcat
     1981 tgtaatacaa tttccatgta tgatcatcat attcagccaa aaagccatca ccactacgtt
     2041 tcatgagata agattctttt tcttctccta acgccgagta cggaatgacc tttattccca
     2101 tttttgtagc catttcaaat gcgcttatcg gtgcgctttt tattccatac tctatgaatg
     2161 tgtcgctcac agttgatttt atgtattcat attgttcatc ttctaagcgc atataacctc
     2221 ctattttctc tctgctaaca ttctcattag ttccattctc tcttccattg acattttctc
     2281 cgcatttctg gcaattaacg ttttaacagt tctgaattgg ttgtcatctt catcaccttc
     2341 acctgtatct aaaagataat cagtagtaac tcccaatgcc ttagcaatct taattaaatt
     2401 tacacctctg ggtacacgat ctccttttat ataatgtgaa atcgctgact ctgtgatatc
     2461 cgtgaccttt gccaattctt tctgcgtcat cttcctttgg aagagtaacg ttgcaatttg
     2521 atctgctact cttactgcca ttttatcctc ctccatacgc gcacaactat ctgacaattt
     2581 tataatacat gattattatc acttcgtcaa gtgtaattat cattttgtta taattcaata
     2641 ttcatacatt ttattatctg cttccgattt taaatttaga gaggcgaagg actgtctttt
     2701 ctatccttcg cctttattga atattatatc atactcgctt aagtttccca gctttaagca
     2761 atgccagcat ctgtgtgttc tgctttgcag atccgacata tccagtgata ccgttttcct
     2821 ttgcaatcag ctttctgttt gcatagctgg agtttactcc gatcgcattc agtgctgcca
     2881 cgatagatgt ggatgttcca gtatatctcg gataatatac cgtagtagac tgcagctttc
     2941 cagtgctgtc cggttccaca tagcagatat ccagatcaac ataactattg atgccgggta
     3001 cattgccgtg gctggagtac tgccatcccc acaaaacatg gaaaatactt ggctttttgg
     3061 atgcagcagg cattgacgta atactcattt tcttggtgct cggatacctt gcaatccaga
     3121 actcacagtc aatgtactcc cgatagggtt tgatataaga attgtaaaat gacagtccag
     3181 tatatacgcc aaactcgtat ccggctgctt cgatcactgc tttgtaggta ttgatgatcc
     3241 tgatcagctg gatactctta ttcttcagac acgcatcctc tacgtccgcc cacaccttac
     3301 caacttttct gccagccaga gtatttacga ctgccttaac tgcggtgatg gcgctgctct
     3361 ccgtagtagt atacagataa ttgtatacat ccacaggtaa tccctgcgct gttgctcctg
     3421 catagtttct gataaaggac tcttctgtct tgttcgattt atcaatgacc ttcaatacag
     3481 caaactcaat tcctgctttc tttacctttg cccagtcaat ggtgccgttc cactttgcta
     3541 cgtctatacc ttttcttgcc ataattattt ctccttcttt ccgtcgaaat ccaataaatt
     3601 ccgaagcagc tcatacatac cagtcgctgc caatcctgaa atcatgccgc ccaatacaat
     3661 atcagcattg atcccggatg acatatggat aatgattgcg ataatcgtcc ccagacacat
     3721 ggataccaaa ggaatgtact tattcggaaa tttatcaaag gctgtcttaa gggaatatcc
     3781 gaccagaaga cagattccta aaatgatagg gttaatgagt tgcaataaaa acgataaatc
     3841 cataatgtta ttcctctctt tctaaatctt caattcggtg gtttgctact ttgatctgtt
     3901 cctgcataac tgcctgcgcc tcctctaatt taaaagtacg ctctatgaca gtgttatgtt
     3961 tatctacttt tttctctagc tgttccagcc gatatgtagt cagttttgta ttgaccagga
     4021 taccgcaaaa agcgcctgct gcgctgcctg cacatccgat cagtgctaca atgatctctg
     4081 ttgccatgtc cttctcctta atataatgag ccggtcacct cccggtaagg aagcaatcgg
     4141 ctcttggctc aatggttgct atgtaattgt gtaggaccgt ctcttgctct cataggctgc
     4201 ctcctactct actgtcaatt ctgtcagctg cgtctccagt tcattaatct gatcccggag
     4261 agcctgtctc tcggcgtgga cagtctccat atcatactcc gtctgttctc ccagcagagt
     4321 atactcatag gtttttatta ccttataatc gcttgcggca atccgttttt tcaggtcatt
     4381 gatctgtgcc gtcacctgcc ggatctgtga ctgtctggac atttcctcca gttcttcctc
     4441 ggtgggaggt tgcaccggtg caacttccgg ttctgcgtag acggatccgt catcggagag
     4501 ttcgtaccag ccttcgcctt ctcggtacaa ggtggtgtag gcttcgtact cgccgttatc
     4561 aagcgggtac ttacaatctg gatccaaata gagacggaat ccgtcagtgt tgacttcgag
     4621 tttatctcca tcatctccgg tgatgcgaat tacatgggga ctttcctctg ctacaatgac
     4681 atgctgtatg gtctttttgt ttttatactt tatataaccc ataagggctc ctttctggcg
     4741 ctcttgtggc tgcgcccgcc atctgattat ctggttacta atttccttcc atcattcatt
     4801 cactccttcg gtaaatggca agttaaaatc cgtagattat gatataacgt tcccatcagg
     4861 tgtaaccacc gattttgtac actgcaaaag aacgggcaat attgtagatt ttggattccg
     4921 cattttaagt gggtctatac catacggatc tccattagcc acgctaccag cagatttaca
     4981 caccaaatat aatatattga tacccagtca gtacttagtg ataaatgatg ttgctagcac
     5041 tggtaatgta agcttgatgt acaatggaag tatatttcag gagtattccg caaccggaac
     5101 tctaaatgct tgcgtgataa aaggtacttt tattatttag ccggtgcaaa tgcgagatta
     5161 tatgtaccac tcgatccaaa tataacggag tctcctgatg caaatggaat acataccgct
     5221 ataggatttt ctgatgatgt acataatgcc aagaaataat ttgcattctt ggatgatcgg
     5281 atagatgccc agccattcac tgcaccctgt attgttccaa ttacatagcc attagtcata
     5341 caagtgtaat tagattgtat ggctacagca gatttatagt caggggcaat taacttgcca
     5401 gttacatcag taaccgcatc tgccaccgcc ttggcatccg ggacatagcc ggtggctttg
     5461 gtggccagca gatcatcctt ggatgtgatc atctgtgcaa aagccggtgc cgtcaaatca
     5521 gtaaaaaact tttttatttt accaaaaacc gtctttacgc tctcgccggt atcaatgttt
     5581 tcccgggcct ctgcctctgt gaaggtgatt tcggagtccc caatatcgct gctaaagccc
     5641 ttggctaaat agatccagtt gatcttatca tcccgtggag ctccgtccgg ggcatccact
     5701 agggcaaggt atgtgctgcc gttatgtaac acagcatcca accgctcata cgcagtattg
     5761 atgttgtaat ctcctttgta ggatattcct atctttccaa gtgtcttata tccttccggt
     5821 gctgccataa atcattcctc cttatgctac tttccaatac aaaacattgt catctgctac
     5881 aaaatccaca cccacgccat ccttcatgta aaggttcatc gtggtctcat ctaaataaaa
     5941 tttaggctct gtgatgcttg cataggtctc cgcacggtct gcatctatct tagcctgtgc
     6001 tgcagatgct gccgccgctg atgcctgctg tgttgctgtt tctgcctgca ctgtgatgtc
     6061 tgcaagatag ttcggctgca gcttctcgtc cgtgatacta ccgtttttaa taccagcctt
     6121 tactttaccg tctgccccca ccgtccagta aacggtatca gagtccataa actcaaactg
     6181 agtaatcaac gctgacagat ccgcatattt ctgcgtgcca tcatccagtg taatgattaa
     6241 ccgctgtgtt tcgggatcat agtcaaaatt aaccgccagc ttttcgagca tggtatcaat
     6301 gacctgctgg gcgccagaaa atgcagtgat tgtccattta ccagtcgctt tatcataggc
     6361 aatctgttta atacaggact gcgcatcaac cttgtcaaac ttggtcaaat caagtgtgat
     6421 tacacgctca tcaatggtgc aaactccatt gctaagctta tccatattgg ttttattcac
     6481 tggtgtcttg gtcgatggct tattctccca atagttctct tcccagtcat acgctttctg
     6541 catcctgctc cacctctttc tgttttgcct cggcagcatc gcggttcgca atctccgcca
     6601 gtaatgcatc cctggctttc tgctcctgtc gtgccaaatt ctcctgcaat gccatgcgct
     6661 taacttcctc cggcaacgga gatgcttcta caaaatttgc gattgcctga ctaaattcct
     6721 tgatttctaa attgctcata tctcttaatc ctccggtccc aaataagtta taacagtccc
     6781 actaatggtt tttgttctcc acgcaactac tgtaccttta taattcatgt acccgctgac
     6841 acccattgct cttacgctga ccagatcaac gcttgatagc ttatttatga tagtcgcagc
     6901 gctgattctg tccgctttaa tcaccccaga ggatgtccag ttggatacct ccatgtagtc
     6961 tgcctttaca gtgctggcgc tgatatagtt ggcttccacg ttgctcagac gggctgatac
     7021 ggctgacaga tcagatgtag tcacatgatc cgcctccagt gatcctacgc ggccactgac
     7081 ggcattgaga gagtcgatgg ttgccttggt ggcaatcagg ttgttcagtt ccagttttgt
     7141 cacattcaac gtctctatgg tggcatattt gctgaccagt tcatccgcat ttaccacacc
     7201 gaccaggtct atccgttctg ccttgatctt gatgctttcc gcagtctgat tgatctctga
     7261 aacgatattg tccttggata ccttggttag aatctgctgt gcattaatgc tgatctgcgt
     7321 ggacaggttc tggttgatat ctttcatttc cagacgagtt tcatccaccg tccttgtaag
     7381 cacatttgtt tttcccttta actggataat ctgcttctgc agtccattaa cctgtccggt
     7441 acggtactcc tcgccctccg ccgtgtaact gtcccggagt gcctgaatgc ctttaagcgt
     7501 acgctgtaaa atataggtat agatggtctc ccgggaagta tacagcagga tatcgtctcc
     7561 aacctccaaa catggattgc cacgggcctc tacctgcgct ggtcggtacc acacaccact
     7621 gatcacgctg aggacgttat ctgcgatggt ctgcaggtct gccgcagact tgccgtatat
     7681 caaaaagtta tcctcgatga tgtaacaatt attgccatta ccagcgatgg caccgatgtc
     7741 gttctcttcc tggcggatct gcagcttatc aatatgttgg actacaaaat cctcatactg
     7801 gcaggagata taattgctcc tggatacctc cgctgttccc atcggatctg cagggtaaag
     7861 gtcatctgcc ggatacagat catccgcggg gtacagaccc tcgatcatct gttccagcac
     7921 cacatactgc agttttccat ttctaccgat gtgcccaaaa catccgttaa tctcgcagat
     7981 ggattcaata accgttttcc ccgggagctc tcccggatcg atagtttttt ctaccaccat
     8041 atcatcgttg accagtgtga tttcttcttg ttccacaccg acataagcac aaaagctatc
     8101 tctgaactgt ctaagcgtca ttggaaatgt caggctgtta taccacccag ctacctctgc
     8161 attcaggagg tcgtacatgg catcataggc cacaatatcc cgatatcttc tatctgccgt
     8221 aggtacatca gaatccactt tataaactcc catcataaaa ggagcctcat cgactccttc
     8281 caatgttact gatactgata tctttttccc tgcaagaggt actacccgtt ccctgaccct
     8341 caatttgaaa gtactcgcct cacatctgcc aaaacttatt tcactctctg agcatagtct
     8401 ctctgtgagt tctgcgcttt cacctttcca gtcctcctca ttcagcacac ttccatcact
     8461 acattcaatc tgcatttttt tggagacaga tgtgtcatta taaaaatctt tatatttata
     8521 atctatcatt tcctctccct cttaatactc ctggaacgca acacgtaatg gcttatacca
     8581 taattccatg ccattccaac tttttgtctc tactgtataa tttggtacat acatttctcc
     8641 cgatttatat cctccggtat ttacatcgaa ataagttaca ataacctttc tttccttctc
     8701 ctttatgtac gctttttcca ttgcttgtag aaattctgtc atttcccatg cttccagtgg
     8761 aatcgtattg aactcaattt ttgttgtata atgatctgca acttcccggt ataaaatatt
     8821 caaaccattt ctgtcagagt ccagatctgc acgctgatcc ggactcacct tataggtctc
     8881 aatatctaca tactttgaaa tatcagtatc tcccactttt aataaccatg cctgaaatgc
     8941 catcctgctg tctccttata catccagcaa caggtaattt ccagttgcct taaagtactc
     9001 cctgtttatc tttttcagta gttccgcaaa ttttacacca ttgatttcta tcgtattgcc
     9061 tgacgccaat attctgatga tagtctccag caatgtaatg atcttatcca gcttttccgc
     9121 agatatggat cctcccgatc ccgctgccgc ctgtgctgcg cttagtgcca ttttctgtaa
     9181 cttatcttcc ggtgatacaa tttctccctg atgtcggtta tcaccgatca tggcaagctg
     9241 aggtgtgttt gccttgacat atccaccatt ccacagtttt ggaatttgcg gaggatcact
     9301 cggcatctca aacccccagt cttttcctac cagatctcct gccttttttg cgacgcttcc
     9361 gattccattt accacattgc gtagtgtaga atatatcagt gaaatcattg cattcacacc
     9421 gtcaatgatc agattacata ctcccttgat agatccccat attgcctgcc agattccatc
     9481 cgtaattttc tgtaagcttt cccatgcctt tttccagttg cctgtaaaca ctccggtgag
     9541 gaagtccaac agtcccccta atattttcat agctccggat ataaggtctg acacggtggc
     9601 gaatacagta cacatgatgt ttatcacaat gtctgccacc tgcttgattg taggtgccag
     9661 atacccgata attggtttaa ttactgtgct ccatgtagct gcaagaaaat ctcctactga
     9721 actaatcagt tcaagaatgt tatcccatag tggtctgagg ttttcttccc atagttcttg
     9781 caacgcttcc ttggcatgat tcagtactgg catcacaata tcattccaca gttctaaaac
     9841 cgtcttcttg atatcattcc aggcatcaac tatgttgccg aaagcactgc tgccgttgga
     9901 atcccaccat tctgtaagtg aatttcctaa atctcctaca atctgaccga ccagtgatgc
     9961 gcattcgcca ccaaattcaa acaattctgt cagtgtacct tccagcagtt cctggttatc
    10021 ctgcatccac tgtgacgtgt gctctgtagc tatatcaaat ccgcttgcaa ggatcgtccc
    10081 tagggacata ccgaaatcat tataacctgt cagaatatct tctagtccct ttgtgatttc
    10141 cggtcttgcc ttatccagtg cccccagcag gttattgtag gtctgctcat taatctctgt
    10201 gagattgatg aacccgtttg ctatagattg gctgacatcc tcgctccagg attgtatttt
    10261 actgctgttc ttttccagat aaccagcaat tccatccagt ccaatatcta ccaacttaac
    10321 tgtaacagcg atcttatttc ctatcctgtt ccccatgtat ccacccaatg gatctatgat
    10381 ggtttcaaga tttttaactg ctgtctttgc cagaggctgt aattgtgtcg ttactctggt
    10441 aaaattcttt ttcagaccgg caaaatcaat cttctttagt ccattattga gctgatcaat
    10501 aaatgtcctg acactcggaa tctttttgaa tgcatcggaa aatttctgcg tgattttatc
    10561 agcactttct tccgctgtct tagttgcggt ctgcagtcct gcaatgtcta ttccagatgt
    10621 ttctcctgct gccgcagatg tatcagtttt ctgggacagt aaatccaatt catctgagga
    10681 cagtaatcct cccaatttct ttgctgcctt cccggcggca ttgatatttt cactgacacc
    10741 tgccgaagca gcctctgctg cttgcattcc cgaagcaaca ttatccgcct ttttccctgt
    10801 aaatttctct gtaaaagcct taaatacatt cgcaagctgc accagtttcc ccatcaatgt
    10861 attgatcacc ttgatagccg gtgtcaatac attgatcagt ccctgaccga ttgccgccat
    10921 aaaagactcc gtctgtaatt tcaggatcct tacctggtta gcccagccgt cagaagtccg
    10981 cataaagtcc ccggatgccg tcgcaagttt actttgcacg aaagagtacc gtagtgcgac
    11041 cttctccgcc tctgacattg ctgctgtggt cttaccatag ccatttgcca ttgcataagc
    11101 gtccagggcc gtctgcgtca tgacgacgcc taaatcctta aggctctctg tttcacctgt
    11161 gaatactgat ttcagcttgg tgtatgcttc atcctgtgta atgttataaa aggatgctac
    11221 atctcctgcc agtccagtca gcgtggtaga catatcgtat gcctgctgtt caccaaatcc
    11281 gaatgccttt gccatagctc cgaaggtacc tgtatacttc tttgccattg tttcggataa
    11341 gccaaacgct gttgaggcat tctgtgcaaa cttatctacc tgctttgaca ttgcaggaaa
    11401 agtcacatct acaacgttct gcacctcgct cagatctgat cccagttcga tacactttgc
    11461 gctgaaatct acgagtttct ttacagcaaa agcggcagcc agtttcttgc taactttcgt
    11521 ggcaaggttc tggatgccgc tcatctgctt attaaaatct tttttattta caacaagatc
    11581 gagtccgatc tgtccaacgc ttgtagcttc actcataacc agcctgcctt ctaagacagg
    11641 cacatcggca cagcgtctta taacttcaac tcaaaaatct ttttacagtc cttattttta
    11701 catcggaaat aaattcccct gcaatgtgca tcttccgtct gcattgcatt caccggatgc
    11761 ccacagtaag gacacactac ttttttctta tctacttttt caatgtatat cgccccctgc
    11821 cagagaaatg aacgcattct tcagttgatc aagtactgct gccatattat caggtgctac
    11881 cttttttgct ctgtttgcac gccattcatt cctgattctg tgttgttccg gagtaaaatg
    11941 gtctaaaata tccttatcct cctcggccct gattgctacg atccgtccca gcggtgtctc
    12001 cggtccgatt ccaataagaa gatccctaaa ctcatcccac ttcatggtat caatttcttt
    12061 tgacagccga atcccgtact gcgcctggaa ggatgatacg atcagactgt aatctccgat
    12121 cagatcatag tacgggtcac tgctctcccg gctcttcgtc tcccgtgatc atgtctactg
    12181 ctgccatgat gattgtctgg aaatccttga actggagatt cagtttatcg atctttttcc
    12241 gatccttctc attaaaaatc agttcatata ccgccaacac ttctttagct gatgtaccct
    12301 tcgaaaaaat acccatgatc ttcagcacag tggctgcatc ggaatttact tctacggtaa
    12361 catccttaat cttcaatacc gggttctcgt caaaactcag cttttctgta atatctacga
    12421 ttttctttgc cataatagcc tcctgttttt atgctgcggg agtaatctca ggttttccat
    12481 tgctcataat atcgaattcc aacggtgcca cagctgtaga gtctcctgct ccaatgttct
    12541 ttacgttcac gactgctccg gcaaacagca ccacggttcc gtcggggaat gtccactgga
    12601 catctttctc tgcagagcgg ccgtttaccc acgcaagtgc tgctacagca tcattaccgg
    12661 catctcctac gttacgtttc gcagttacgg atatggtaac tcccttactg gtaagcaggc
    12721 gtctcaccca tcctttttct gtaaacggat gccattcctc tactccatta tcgaaagata
    12781 cactgaatgt ctcgcagtcc gcaatatcaa ccattttctt ttctacaccg cttgctgccg
    12841 cattgatctg gaactggttt tcatagcatg gatatactcc tgtaataggt gtgctcattc
    12901 tttttcacct tttcctttct cataaataac agccatctct atgacccatt cgcagatacc
    12961 ggcatcatct tttccgacat cctgtggttc ataaagaggc tgtataaatt ttatcaactg
    13021 atcgttgacc gttacatttc ttgcagcctt caccgcatca aatgctgtca tggctgcctt
    13081 ttctgattct ctcggcgaat tattccagtg aatcaacagg gtgacatatt ttgtcccgta
    13141 agatgcaagc tgtggtcctc ctaatgctgt cttatactcc tgctgatgtt tgctgttata
    13201 aacaccgatg gacttctcct gcttgtccgg caggcttccc atatatacat ggtctgccag
    13261 ttcaagggat tccacataat cccgcacatc cgataacatc ataatccggc aatcctcctg
    13321 tatatttgtt tgtatgcttt ctggcagtac tctgattttt tcccagagat ccagtcctca
    13381 taccattcgc ctcttgcatt cgggttctcc gtcttctgga aatgatattc cgggtggaaa
    13441 taaagccgtc ttgcataggg tgtgctggat atgatactga cttttccctg gctgctctcc
    13501 gaataatcaa caaaagtgct ctcgttttgc agattgccgg tatcccttgg gaacacctgt
    13561 gcctgcacta cattggtatg caacgcctca gcggtctgct ctaaagccac cacctgtgct
    13621 cttgtgagct gttgaatctt cggaaaattc agttttactg tagaatttac actgatcata
    13681 tcagcagcac ctccgtatag ttgactgttc catccgggtt tctcgcctta cggccctcaa
    13741 gaatcctgcg ttttgcaccg aatatcaccg cactgcctcc tgatatgacc gtaagctccg
    13801 ggcatatatc tccgggaaac aatgctgtcc ctgtaatctc tatgagtttc ttctccgatg
    13861 tcagcacagt cttggcttta tcctgatagt tacattttcc agaatattcc accggcttca
    13921 atggctcccc gtatttgttc agtccttcct gatctatcgc aacggagata tctgtcttgc
    13981 ataatctttt gggcaccaga cacggatatt tcatggaatc acctcgcaat tctgcaacac
    14041 agacccgtct gcatcagcaa cgaatagaca tcccgcttca tggcaatacc tttttccatg
    14101 aaaacattcc aggaacttcc gaactgtgcg gatactccat ttatgctata gctggataaa
    14161 atcgtattga tttcatctgc attctcatat tcgaaatctg cctgcaggca gacaacttct
    14221 tttatcgtct cctgttgaaa agctgtcaga tgatcgaatc ctgcagccac aatccggtta
    14281 aatgtcaggc tgtcaatatg tcggcatgcc tgacggagtg ccttttcaag ctcttcgtca
    14341 ggaatcacgc tgccattata gatctctgtg tactcttctt ttcttacata aggtttatag
    14401 gacatatgcc ctccttactc cccggtgtac tccgtggtat ccacatctac atagacgcta
    14461 tccactttac cgtcacgacc attcgggaat acaaaagtgt cagacagaga tctgttctgg
    14521 tacaggtatc cgtctccttc ggtatgtgtt ccgggattga aataatagat agaagcgatc
    14581 ttgggaaccg tcttacaggt ctgtccgcat gccaccagta cattgatctt atgagctccg
    14641 gttacagcag caacgtggtt actggtgtcc tcggccacct ttttcaacgg agcaaatcca
    14701 ccctcagcag gctcccagtc gaaagcatca tagaaacgct catcgtcgat aacttccatg
    14761 atgggtacac catcgatttc cgttactctt gtctcgatgc cgataccacc ctcagcaatc
    14821 tgtgtaagtt caattttacg ggtaaactca gtggactgct ccagtgcatc cataatagta
    14881 ctggccacat acataagcag gctgccattt gccttatacc gtctcaactt tcctttcgca
    14941 aggatgtcct tcagcattcc gaataccttt gccttagtat aagcagaaat agcggtctgg
    15001 ctgtgatatc cctctgtctt ctgtgccacc tgtgccacac gggagaagaa cagggcatct
    15061 gtctcaggca ctacctgagt ctgttcgaag gttctggaga tattctgcat ggatgcagtt
    15121 gcgttggtct catccacatc tgccttgtct accaggaact gaacgtctct gtcatgggtt
    15181 actgtaaacg gaacatctgt ctgatcgaag gatcccatgt tccaaccacc ggttctcttg
    15241 tgattcttat aaccagtggt gctcatctgt gtaaagtgga atgtcttcgc atccagccat
    15301 cttacattag atgtaatgaa gggagaggtt aacgctccct gcatcagaat ctgcaggagt
    15361 tcaggactcc actgctgtgc atagtttaaa ttaggcatat cttatacctt cctttcctta
    15421 gttccaccga ttccatcttt tggtcggtgt ctgttgctgt tgtacggttg cctgctgtgt
    15481 atgctgcgaa ggatctccgc ctgtccctac atgaaggaaa cctgtagtat ctgtctcctg
    15541 cggctttaat gcaggaatat cctccaacac cttattcagg gcttccgtaa gtttctcatt
    15601 gctgatcttt ccatcctgtc ctactgtctg gctgaaatct gccatcttca gtacataggg
    15661 aatggatgtt acgctgattc ccagtccgac tgctgccatc gtcgctgcct gctggatctg
    15721 tgcctgtctt gcctcagctg ctgcggttgc agcctgctgt tgcaatgctt ccacattcgg
    15781 ctgatttgcc gccttctgtt ccttgaaggt tgctatagcc tgttccacct cctgttggga
    15841 aagtccctgc tgcttgaaat aggctttcaa tgccgtatcc tcttttgccg caagcgttcc
    15901 atccaacatc tgctggattt tcccatagtc aatctgcggt gttgcattct gctgtgactg
    15961 ctgatcagtc tgttctcctg ccggtgctcc gccctggctt ccatcggggt ctaagaatct
    16021 tcttactgtc ttgtaaaaca taacgtgctc ctttccattt tgagggtgtc acccttactg
    16081 cgatccattg tcttcggtgt ctccggtcac gctgcagttt attgccttgc tcgtgtttgg
    16141 gcataaaaaa acacgccatg aagcgtgttg attccagatt atttgttgca ccggtgcaat
    16201 tttctttttt cgagataaaa ataccaccaa tctactgacc ggtggcttca tgttctttta
    16261 ccattcttcg caaacgttct ttataatcct catagctttt atcttttccg atgatgtatg
    16321 cggcatctcc cattttttcg gagaaggata atactttcct gcgcaactcc tgcagttcct
    16381 catcgttttt cattttttca acaaattctt ttttgaacat aattacctct ttagcacttt
    16441 cataaatgct tcatatagct ctggcaattc actttttatg aattctacag ttatatcatc
    16501 cgactgatac aatgcagcat atatatccgc aaatatctcc gactccgcat atccgggttt
    16561 acctatgtat tgtgattcat gtctgtatac tcctgtaatc acattgtctg ttatgcatga
    16621 cattatatca ctgatgaagt aattgtactc taaatcacca tttacagcaa gtctccgttg
    16681 atacttctcc tttttttgca atattttgtt ttctgtattt tttattgcct ctgcgaattc
    16741 agcatacatg ggactgccat actcattatg atcaattcta tgggctattt catgcgccag
    16801 cacatgcttg tagttctcct cttcatactg cggatgtctc ggattgataa ttatcaaatc
    16861 attatcaaga tcatacgaaa atgcatattc tgacagttca tctatcttga tgcactcatc
    16921 tcttgtgtac tgatccacta aatcgatcat gatctgcgga gtatccgatc tcggcacttt
    16981 cacctcatca ggaactttat accggtcttc cgtttcctga ctccattctt tttccttcgc
    17041 acggtacttg cttttattct ccggatccag tgaaaatgat gctaatctat ggaatttttt
    17101 ctcctgtctc tctgcatatt gctgtcttgc ttctttcctg ttctgttctt cgatattttc
    17161 tatgtctttt ttactgtatt cattatccaa atcctccagt tctggaaaat aggtagtgtg
    17221 gctgtctctg catctagggt ggtatagtcc tgctgctatt gccgcgctca tcaggggata
    17281 tggtccatcc ttggcgcttc caccgctcca tacatcatcg atcagtatct taccaacaaa
    17341 cggtaaacac ttggggcagg gatttccacg cttattcatg atcaccgtgg atatccccca
    17401 ttcctgcctt ttctgccctt ccccctgcag gtatgcacgc ttactggctg tccgtattgc
    17461 catgtccgca tagtctgcca atgtgtgtct ggatccattg gtatattcca cacagttaag
    17521 accagcggca atgaaatcct ttgtggccat gtctaccgcc ttctcataag tccctgctcc
    17581 actgttggca tatacctgag cattaaaaat aatcttacga tattggtcat ttgccatgcg
    17641 caggacggct gtctcagcct tttccatgtc tgatgtggtc gcccggatca gcgcctccag
    17701 cttcctctgg ttcaaccgga agaatgccgc cgatgctccc ggacttactc ttcttgctgg
    17761 gaaacctttc tttatagcct ccagtatggc tatctcctgc tccatatctc cttcatccct
    17821 ggcagtactg atcagcgctt cgatccggtt attaatgtct ttaaattttg taccaaatcg
    17881 ttcctgattc tcttttctgt acttttccaa cgaccggagc tgctctgtct gccacatgga
    17941 ccactgctta tcttcatcga tttcctcaat cttatgtctt cgcatattcc ggatcatgga
    18001 agcaatgagt tcattctcaa tagcttcgaa tgctgctccg atatcatatt ctgaatttat
    18061 cttaggcatc taatcacctg ccgtttgcat ataccttgaa tccctggctt ttaaactgtc
    18121 tggtcaatgt cttgatctgc gtgacgctgg tacaatgatc acatcggagt tccgcataat
    18181 tacctttttc cactgcatag attcctttcg gtacctgctc actggccacc ttcagaagcc
    18241 cctggtactc ctcccggttc atccggtatg tttttttcgc tacttttact tccatcacta
    18301 cctccagtaa atccgtttat cctgaattct cctgcatccg ttctgatctc cggctccgga
    18361 atgctctgaa tcccctgctc tgccttgagc cttgcgattt cttctcgttt gcaatcatcg
    18421 tccagactgt caccgtatag ttcctccaca cagcgctcaa tgctcatgat tccgctctgc
    18481 tttgccttac caactgtttc cacctgagat tcaaatgaag gattggcata ttctccaaat
    18541 gggagattta cctctacact ttccactgcc tcattcttca tcaggtgata tgcgttgata
    18601 cacatggata ctacctgtgg caatactgtc tgaagagttt ccacgataat gtttcttgtg
    18661 tacagcgttg ttttttcctt ttcacgctgc gcttctgcat tatccagttt ttttacatca
    18721 atccccagtg tagaaggact gatgatcccc tgcaggcaaa ggtccagtgc cgtacagtag
    18781 gaagcctgat agctgtcatg aggaatgctc ggctggtctg tactgattac gtttttctgc
    18841 ccttcgcgct ggtctccttc tgctgcaaaa tatctgttat cgaacggatt cggtgttatc
    18901 gcagctcctg tttccggatc cctcggaacc agacagtccg gaatatatgt tttggctctt
    18961 cctgctctca gcgcatccat ccactggctc catacttcat ccagcgcatc atagttgtcc
    19021 acctttccgt caaagatact tccgccacgt ccttcatatt ttgccgactt atagaacatc
    19081 ataggcaccg ccagcataac gcttttatcg aaggtcacat cttccagtga attggttatc
    19141 tgtagtgtag tcagcggaac ctgtctgtta tccagataca gttcgttctt tacataccca
    19201 tatccatata cctcattgag cacatatgtc tttcctcctc cgctgtatgg tgtcttaaat
    19261 atcacttccc ggaccttgtc ctttttccgt atgatttcga cacgatcccc ggcataccat
    19321 tctaaaatcg gatactcact gacttctgta tcaatggaca ctttaaaagc cccgtctccg
    19381 atatacagtg cttctttgat tgcatcctct accttatcgg caaagttatt attctcaggc
    19441 tttgcaatgt ctttccatat ctgtttctgc atttcgttct ctgaggaaaa ttcaaattcc
    19501 cccatatctg gaaggactac tgctgccaga gttctcaccg taagctccgg aacacctgtg
    19561 tggatcttgc gcatttccat ccccggtgta ctcttgctgg accagaattt atatttatct
    19621 gcatattccg cattctgctc atagaactgc tccagttcgt tgctgtcacc acgataccag
    19681 atgcggtttc ggatcgcatt cccctcgaag tccatcatct cattgatatt gaacacataa
    19741 ggattcgccg gagaaacatt cagccagctc cgtatacctc ttttgatatt ctcatttatc
    19801 ttttccatca ggttcacctc tgtttatcct cctcgaatcc aatcatattc cggtatggaa
    19861 tccatccgta ctggtttgca ttgatcgtat ggtcgttctt atcctccggt accgggacat
    19921 cctcttcctc gtcccatgaa tagcgttcca attctgagat atggtttgtg caatcctcaa
    19981 ctaccagata gcagtcctgc tggatccatc ccagctgtaa attgatacga tccagtattg
    20041 ttaccttttt gtaggactca atgaaattgt aaaggcaccc atgcaggcgc ttatacttcc
    20101 gaagttctgt tattgtcgcc gcatccgcgc agtcaacaaa ggattctttt gcaaatcccc
    20161 attccgatct gcatctatcc agaaaagcta taaactttac cgttgtgtca gatggtgcca
    20221 acggcacact gagatcagca ttgctataca ccatttcagc cagtgtgatc agcttgcggt
    20281 catccgtaat gccctggaag atcattgcaa tggtatccgg agattttgag gaatatgatg
    20341 tatccagtcc ggccgtaaac ttcctgaaac ggatcttccc atctgcaatc tgtttcttca
    20401 cccatgcagc agtaacaaca tgtttctttc tgacaaagtt ggagaatacc aaccctgtcg
    20461 cttttccgcg gagaccctgg atcttatttt tccagatctt tgttcccttc ggtgtgtttt
    20521 gcaggatcat ctgcagttta tccggtggaa ggcctgcatt gtctttaaaa gaaaagaacc
    20581 aatggatcca tccgtccttt ggctcttctt tcagttcctc tatgatttcc tgtggtgtct
    20641 catccttcca ttccggaaga ggacgtgcac agttgatata ttctttgtac accggcagtc
    20701 ccggatcgtc tgggtttagt gttgccatca gataatcaca tctcatggat gcttctctga
    20761 caaaatctat gtctgcggta tttacttcat ctatgtacag acagccatat tgtccaccga
    20821 gagctttctt ccactttttc ttgttaccgt aacccagcac atatatgact ttatctcccc
    20881 ctgcagtatg gaaaagcaga tgtggaatct tatcatcctt tgtaccactg ccgttatact
    20941 ccaccaaaat accaaaatca tccagtatgc ccagatcctt gttgatgata ttcttctctg
    21001 cggtaccggt atcatctgca gcaatgatgt gaagcttctt ggggctttct gccaccttaa
    21061 gcataaactt gaagattcct accgtcgtct tacctgctgc cgtggttcct tccagaaatt
    21121 ccaccggagc atcgcatttc aggaatgctt tgtatttctc tgacagcagg agtttacttg
    21181 cgctcattac ccatcaccac gcatctgtct gatcaggtca tccagtttac tctgttcgga
    21241 tttaagctct ccggagatct ggacatcctg tttgtctctc catttatccg gtttccggtt
    21301 ctttaaccag aatatctggg ctgtggtttc tccgggttta tgtttctcta cttcgacaac
    21361 taccggttct accgtttcct tgattctctt gccattttca tataggactt cttttttcgt
    21421 acatataacc ttttcagtat aattgtatcc agccgcatta ttatatagag cccgttctat
    21481 ttctctatca actacttcct ttcccctttt tagggtgtta gaaatgttag tatacttttt
    21541 acaccatgca tataacgttt ctctgcgaat tcccatattt gtagcaatct gctcatctgt
    21601 taatccatct cttgcccatc cttccagctt tagcaagcct tccggtgtca gccaatactc
    21661 atatttgcct tttgccatcc gctcaccatc tctctagtgt tgcaccggtg caactccacg
    21721 aaaaaaggca acgcagctat ctgcattgcc ttgtcactaa tttatcacga tactatatta
    21781 tcacatttga catgcgaaat catgccatct tttactttaa ctctccaatg tacctaccaa
    21841 tctgttcgat tgtcttgaac acgatccgtt tcatctgccg ctcactgtat gatgccccac
    21901 caatcttaag gtacgggatc ggtgctctga ggcccttgct ccagtatctg atccggatta
    21961 ctctctgttc ctccggtttg agagaattat aggcaaattc caccgcctct atctcttttt
    22021 tgatcctatc atgatacacc gaggtcatct tcagggcctt tgcttctgtg acagattgtg
    22081 ccttgtccat ttccctgaca gggtccaacg gacgactgct gcctcccgct ggcgatgcca
    22141 taatgtccga tatgtactcc tcatattctt tcttgcgctg gggataacgt aataatatag
    22201 tttcaatgat cctccagctc gttctgttaa ttctctgcat cgatgctttc tcctttctgc
    22261 cacaccggag ttgcaccggt gcaattatgc cactttgttg tatttgttct gcatctcctc
    22321 gatatcatct accagataat attttactgt aatctctggg ttggaatgtc cgaggagctt
    22381 acttaccagc atcacatccc ctgtcttacg gtacaggacg gacgcaaagg tcttgcggta
    22441 tatatggacc gttgccgtca accgcgtcac accaccgcgg actgccattt ccttggtcag
    22501 cttctcgatg ccgtactccc gcatacgatt atgaggcgcc ctgtcagcca aaaatacagg
    22561 gtctgtccca ggacgcccac cgatataatt ccgcaatgct tttactgctt taggtgtcaa
    22621 cataccggtg cgatatttat tgctcttctc tccccagatg ttgacagtcc tgtgcgtcag
    22681 atccagatcc gaaatattga gattggatat ttcgcctact ctcatgccgg tgcaaagcat
    22741 cagttcaaac agtgcttttt cctttggtgt atccaataca tcacggatat cttccacctc
    22801 ttcatctgtc aaccgcttct tttgtgcctg aatctgcttt accttgtcca ctccatctgc
    22861 aatattatca acgatatgct ttttccggaa agcccagccg aaaaatgtgc agaggtaccg
    22921 gtatagcgta gacttataat tctggctgat atgatcacgg tatgctttga tagcaaggaa
    22981 atctgttatg tcctgtgctg taatatactt ataatttttt cccgtgaaat cgaagaactc
    23041 ttttattctt ccaagatatc cccgcatggt tgattccttc aggcctactg ccgccccatc
    23101 gatccagtat cgcttcataa gccattcatt gtcatgttct tccaccatcg gaagctgtct
    23161 ggtctcggca agatcaaagt cctgcatctt taccaaaaat attatcttta ccttgtcaat
    23221 ctgttccggc gtcaaaatgt tactcatttc aaatgcaatg tcgttgatca ggtctgtctt
    23281 tgtcataggc tgtacctcat tttctcattg cgtgccagct gatcctatgg tatgatactg
    23341 gcaagcaggt aagcggtaga tgctatcttt ggtcggatgg tctaccgctg ttttattggt
    23401 atcgattgca ggccctctgc actggtcttt ttattatgct ttttctatca ctccttcgct
    23461 aaattccagt tgatatttca catattttag gaaatttaat aactgttata tttaattatc
    23521 aaattcttcg caaacccttg aaaatactaa gtttgtagca agtgagcttt cccttactct
    23581 ttcccttgtg ttatattctc acattggttt tcatgaacct taatatggga aaaaataagg
    23641 gaaagaaaat ttcataacac agtataacaa taacgacatg gtgaactgat tgaaatgttt
    23701 tcgatattta actctataac tgattattaa aagaaaatgg agataagata cgatgagaac
    23761 aatttatgca gaatacaaca taaatcacga tagtattgat gtttacacaa gtgccggata
    23821 tatgcttcgc attgattgct ggaaagctga aaaaaatcta aaaaccacat acggatcaga
    23881 atgtgcgctt acttcattgg ctgtggatga gcctttggaa tatgcaagat tatatcttga
    23941 gggtaattta cagatgtggg tagatgcaga agactcacta gagttataat tcttatccat
    24001 ataaataata tgcaaatata tatcccatca atatagcgaa tatactgaag taaaagtatc
    24061 tttataatca attttaacca aaagaatatg ttcttagagg aggtgtctcc cgaaagaaca
    24121 tattcttcta ttatgggtat tataaatata gaacatgctt ttcttgtgct acaatttctt
    24181 cactatgagt tacaacaata actgtttttc cctgttcact taactcatgc aatatattca
    24241 taacaatctc actatttttt ttgtctaagg aaccagttgg ttcgtctgct aatacaatcg
    24301 aacatttctt tagcattaaa cgagctaacg caactctctg ctgttcgcca ccagataatt
    24361 gatagacttt tttgttaatt actttctgta aaccaacttt ttcaagagct tcatttatta
    24421 aaatatccgt tctacctgat ttttttatta tttctagatt ttcttttact gttttgtttt
    24481 ccaaaagggc aaaattttga aataaaaatc caacaacttc tttgaaatat ttctgctttt
    24541 ttcctttttt tgccacatca aaaccattca caataataga acctttgtca ggagtttcaa
    24601 tacctccaat catattaagt aaagtacttt ttccgcatcc actttcgcca ctaattacca
    24661 aaaaacggct atctggaatt tccaaatgaa aatttttgaa caaaatttta tcattatatg
    24721 ctttgcatag attttcaatt gttatcataa gcaacctcct tttaatgatt tcggtatgtt
    24781 tgttttttca acaattgtga tattaaagaa aataattgta aattctatta gtgccataaa
    24841 tgaactaaca agaatgctaa ttcctacttc agtctgtacc gataataaag aatagataag
    24901 catacctact aatatcacaa aattttccac tacaatcatt ttaagaagtg ttttgtgccg
    24961 ttcaaataaa ctatatccaa agattttcat caaggaaatt ttcattgcat tttctcggaa
    25021 ctctagtcta cttacggtaa caataatcat aatatttaaa agcaatacaa tcgtacaaag
    25081 agaacttaaa aatcccacga gttttattaa aaatgtgtga ttatataaat actgttcatg
    25141 tacgtttgta attactagct gataatttcc aagcatatct tcatactttt tgctgatatt
    25201 tcttagctgt ttttcatcac attgaaataa aactgctcct gctttatagc tttcaagata
    25261 cccaccattg actgctaaat ctttgtttgc ctgataaata attatcggat ttttgctttt
    25321 ttcgatacca tttattctac ttgtatctaa ataggaaaag tattctgttt cgctatactc
    25381 tatgtactga atatttaatt gttgtaaatt atcatgattt aaaacatggc tcaaggagtc
    25441 ataggctaat tgcttgtttt tagcaaaata tctattcttt ggaatgaaaa taatcaaatc
    25501 agaagattca ttctctactg catttatctg cttcgtaaaa ccttgtaaca tatcttttgc
    25561 atgattattt acatatatca catcattttt atcatttaaa atattcaagc atattacagg
    25621 ctttaatgtg ttatattcat tcttgtatag tttattccaa aaagcttctt ctttttcagc
    25681 attaaaatca gtagtcttta cggtaaaata atttgcatca taatattctt ctaacaaatg
    25741 ttcatttgta aataggttat tatgaatact actaatatta gtgataattg taaaaacagc
    25801 ggctacccct gctataaatt ttaacgaata aattaaaaaa tccactcctc ttttgtgtgt
    25861 tgcatttgcg aatgcctttc taatatcaaa aaagcaaaaa gaacaatatg gaatagtgga
    25921 aagaataatt ccaatggaat ataaaattgt gacgagtcta ttctcatatg ctcccgatat
    25981 ataatttgac agcaatattt ttgcaacaat aaataatgca atgtcaaaag ttacatcgaa
    26041 taaagcagcc ttaaatgcta tgaaaccagc actttctcct aaggaaactc gtacaacaac
    26101 ctcttttttt cgcctaacca cttcaattac atttagaaca atcatcaatg caataatcat
    26161 accccatata ataaaaatca tgtccttttc cgtagaattc caatattctg gataagttaa
    26221 gctatatttt tctgataatt tctgatatgc agagataata ttgtcctcat ttcctatgta
    26281 tgagataaag ttttcataac ccacagaggt actttgcaat tctgagagat tatgaaattt
    26341 tactttcgta attccggaaa ctaaagcggt atattctgat tcttctatgt ttgcagtatt
    26401 ttttaatgtt tgtctgatta ctttatcatc gccataaata tgaagtgttg aaaggtattt
    26461 attattgatt tcattgtact gagaaaaaat atgtacatca ttctccgttg cagtttctga
    26521 tacatctttc agaaactgcg acatatcttc actagcaact tcatagcgtg atgacgtaaa
    26581 atatgctgta gaaaagttcc aaagctgatc ttgaaatatt tcagattgca taagtattcc
    26641 aaaaatcagg aaacaaaaca aacataagtt tttaattgct ttcatttatt tcaaccccag
    26701 attccatact ttatttgctt tttcaaatat cgtagtcaat attgtgctat tttcatcaac
    26761 aatccattga tcttcttgat cttcagcttt tttgctactt gacaacataa tttgagtatt
    26821 caaatcctta tagtcattaa atagcacgcc ttgacttatt ccttggtgaa atggttttat
    26881 ccaaataatt agtgcatatc gaacatctga ctcagctatc gcaagatttc cattccaaag
    26941 caaataatca gggacagaca cactatactg gaatccatct ttatctgtgt aaatttttac
    27001 tccagattct tctatttcag ggaagtcttt ctgaaattcg ttatatggtt tataactccc
    27061 gaagtaccaa aataaattat agcaacagaa caaaataacc aaaacaattc ctatatattt
    27121 tatttttttc ttcatttata tacactccac aaaaaagagt ggttggaatt ccaaccactc
    27181 agccattatc ccgaaccttt gtttagtagc tattaccgta ggtaatacta tttcctttat
    27241 gagcaacctc aattttagac caattacctt taccaacatt tgaacccgaa aaagaaccat
    27301 ttccattttt gacatatgcg taatgatctt tagttgaatg atatgcgtgt gcataatctt
    27361 cattaatagc aagagtatta taaccataag tcaaaacacc ttttccgcca tcagatgtag
    27421 tgctcaattc tcacttttta tcaaatgtct tattcccgtt tgtaacagaa aatgccatta
    27481 ctggaattga tgaagaaatt aacattgcac taatagctac ccctacaatc actttctttg
    27541 ctctcataag ttttacctcc tttgatctgc tgtatttcta tttatgtttt atcaaatatt
    27601 ttgtatctag tcaatcagta tcttgactta tatagcgtcg aattgtaaga tatattcctt
    27661 gtaaaattaa ccaaaagaac aacagattta ttgataatgc ttttttaatt acttctccaa
    27721 aataaatgat gacagccgca cttgtcagta tcatcagtag ggtaataaca tcccaacagt
    27781 ttttcttata taattctata acctttattt ctatcagtat cgccagtatt cctgtccctg
    27841 ccacacatat tcccacaacc aatatcagca ataaccaata tatgattccg gctataatct
    27901 catttggaat ttttgtgctg atttgtgcca cagattgccc ggcatcaatc gtccaaccga
    27961 taaaagtttg tatgaatgat gctgcatcat ggaagaatga tttgcaatcg gaaaggaaca
    28021 catctgactg tactgcctga aaaagagtgg ttgtcagcga ataccatgca agaaggaaca
    28081 aggttgtttg gaacattacc gtttttgctt tatactgtcc ggcaagtcgc tctttttgtg
    28141 ttccgtattt tgctttttca ttctgatagg ctgtccggtc acaggattca catttttcat
    28201 agagtaccgg cttttctacc ggtatctcta cgattttctg atgtgtccgg gcataatccc
    28261 gttgttgtgt taagcatcgt attttatctt gacattcctc tatcgtgacg tttgcgcttc
    28321 gcagcttctc tttctcgctc cgcaatgctc tgtctgccag cttcaagtcg ttctttaatg
    28381 cttgaatatt cccggctctg ttctctttgt ttaatttctc gttcaaggtc agagattcgt
    28441 tgagctgcgt cttcagttcc acgataagct ggtctttctg ttccagttct tcttgtatct
    28501 cctcggtcaa gagcagaagt tcttccaact gttcggcgtt ctttgattct ctggattcgt
    28561 tcatctatat cacgtccttt ctctacctgc tgttccagtt cagcaattcg gtgttctgtt
    28621 tcagtaataa actgttttcg ctgttcagct tctgtgccaa tatctgccat tgctgatttt
    28681 cgttttccaa aagttctatc ttcgttttct gttcttccat cttggaaagc agttcctcgg
    28741 tatttggcaa aatgttgagc agctcggata actcgctgtt taatttttgc aatttctgat
    28801 tctgttcctg cataagagag agttgggtgt cccggttctg catttccaga agcatttctg
    28861 tcattaggtt ctgctgttct tcgatttgac caatcatgga ttccattaag ggtataattt
    28921 cccggctttc ttctaatgtc atttaatcgc tccttccata atgataatgc acctgatact
    28981 ttcccaaagg tgtccagtat cttttgcaat aacgtgtttt gttgttttat gatttgattt
    29041 tccgctactt tccacgaacc ttttatttcc tgcccggcaa gaaacttctg ttcaatcttt
    29101 cttgcgtcag caccttcatg gatggtagga atctggagtt ttccctgctt ttcataagaa
    29161 cggtgatcga cctgattatg caaaggcaga tgttcattac acacctttgc ccactcgctc
    29221 cgccacagtt cacagttttt tggattgttc catcccgtag catcggtcag tacccgtttc
    29281 cattgcaggc ggtttcttgc accaatcttc tggattccgt tttcatctaa tacagggata
    29341 cggattccat gacggtcagg gtttttctta tcctgccacc agttcggatg ggattcatca
    29401 atcacgatat ttccgctttt atctctgaca aaaccccaat ctttgacttc tttcttcccc
    29461 caagaatgat ccgggttaaa agggcgcatt gtcagaagca gatggacatg aggattgcca
    29521 tcccctttgt cgtggatact ccaatcagca cacattccct tatccacaaa tgtttttttg
    29581 atatagtcgg ctgtatattg aatctgttcc tgtctgctcc attctttggg gagggcaaat
    29641 tcaaatgacc tgccaagttg ggcatctgca cttttttcaa tcttcaatac ctcattccat
    29701 aaccgttgtt tctgaaatgt gattttaaat ttttctaaag cagcttcttt atcttctgac
    29761 cttttgtatc ggacagattt ttgaaagcgt tttatatttt cttctggtac tatctgccat
    29821 tcaggaggtg cgttttcgca catcatcaga ctggtgtaga ccacttcttt tttagaagtg
    29881 taataactga ttctcccggt ttcctcgttt ttcatcacat ccccattgag atatgcggaa
    29941 gcggagataa cagatttccc tttacttcgt ccgactattt tgattgtgta atgaaaaatc
    30001 gccatgtctg gattcctcct ttctgcacat ccggcataga tttccggcaa cattgctttc
    30061 agttttagaa aaagcagtat tgccggaacg ccctctgcgt aagagtgccc tgcgcatagc
    30121 agcctgcatg gaatgcgccc ctctggcgaa gagtgcctcc tgcggcatga gcaggtctgg
    30181 ctgaaagccc cgccgacgga acgagcccgg caagcgtgag cgcagaccgg ttgccacttg
    30241 tggcaaactt attgggacga cctctggttg tccataagtg cgcccttcat gaaaaagcaa
    30301 tgaagggaat gaaaaactca tcccctccgc cattacactt cctccatatc ggtatttgtt
    30361 tctttctgca ttgcctttga gaaaaatttc ccattggctt cctgcttttt cagaaaacca
    30421 atcagctttg ggatgtcttc ttcctcaata ggcgcaccga gaacggattc caccgcaccg
    30481 ccaacctgac agagcctatg ggttcgtttt ttactttctt cggctttctt tcgtttcaga
    30541 agttctttct tctgtgctgc atatcgtttt gctttttcaa ggctttcctg ttccttcttt
    30601 tccatttcaa gcattcgttc ttcataactt tttgttgatt ttgccattat tacattcccc
    30661 tttcttttgc aaacataagt tctcggttgc gtatcagaag aagcacatgg tataatcttc
    30721 ttgcgtgtag gtatatcatg gcttcggtct gatagaacct ttggcggttt ctttggaagc
    30781 cgccttttta atttgccaca gttcttgtta cggcttttac atttcctcta tccctcaaat
    30841 cacgaccttc attagcttcc ataaacattt ccagaatatc ggttttaatc aggactttgc
    30901 gaccaacctt taatactgga agtttcttcc attctacaag ctgtcttaag gtgtttctgc
    30961 cgattcccgt atagtcagca gcttcttcga tggataatgc aatttttctt tgcgtcatat
    31021 aatcacctcc ttataaattg cattatgcaa ttcttgtgat gtaagtatat ccttttcaca
    31081 tctttttgtc aagcgttacg aaatattaaa aacaaatatt atattgcata ttgcaattat
    31141 agaaaaacaa tgctataatt catacatacc gaaaaaggag gcagtcagca tgaaagataa
    31201 agaactacgc aagctgatag gcagcagagt aaaacagcgc cgtctggaat tgaatctgac
    31261 acagccttat gtcgcagaaa agatgggtgt taccgcttct acaatcctgc gttatgagaa
    31321 tggttcgatt gacaatacga aaaaaatggt gctggaaggt ctttcggaag cactccatgt
    31381 atctgtggaa tggctcaaag gggaaacaga tgaatatgaa accgacatta cggataagag
    31441 agagttacag attcgtgatg cgatgggaga tattctggaa cagttaccgc ttgcccttac
    31501 caaagaagaa gatgcttttt caaaagattt attactgctg atgttaaaac aatatggtct
    31561 gtttttggat tccttccagt tcgcctgcaa aaacttcaag gggaatgctg gtcagacgga
    31621 tattgccaaa acaatagggt ttgaatcgaa tgatgaatat aatgagatta tgttcttaag
    31681 ggaaatcact cacaccatca atgcttttaa tgagatggca gacgttgtaa ggctctattc
    31741 caagaaacca aaagcagcag aacaaaggct tgcaaatctt ttatcagaag tcttatacga
    31801 agattccgaa tcggtatagt aagacaaccg gagttatgat atacttacac gcacgaagca
    31861 tttcatcagt tccgattgtc taatatcgaa aggagacata cgattatggc aaaaggatct
    31921 gtaagaaaaa aaggaaagaa atggtactac cgcttctatg tagaggacgc aagcggcaat
    31981 cttgttcaga aagaatgcgt tggaacagaa agcaaaagtg aaactgaaaa gctgctccgt
    32041 caggcaatgg atgattatga gaaaaagaaa tttgttgcca aagcggaaaa tctcacagtc
    32101 ggacaacttt tggatgtgtg ggcagaggag gaattaaaaa caggtacgct cagcaatggt
    32161 acggtggaga attacctcgg aacaatccga aatatcaaga aacacccatt ggcagaacgg
    32221 aaactgaaaa atgtaacctc tgaacatttg caatccttct ttgatttgct ttccttcggg
    32281 ggagttcatc ccgacggaaa agagagaaag ggttacagca aagattatat ccattctttt
    32341 tccgcagtca tgcagcagtc cttccgtttt gcagtatttc caaagcagta tattacgttc
    32401 aatcccatgc agtatattaa actgcggtat cagacggatg aagtggattt gttttccgat
    32461 gaggatatgg acggaaatgt ccaaccaatt tcacgagaag attatgaaag attgcttgct
    32521 tatctgcaaa aaaagaaccc agccgcaata cttccaatcc agatagccta ttatgccggg
    32581 cttcgtattg gagaagcctg cggtttggca tggcaggacg taaatctgga agaacaatgc
    32641 cttaccataa gacgcagcat ccgatatgat ggctcaaagc gcaaatatat catcggacca
    32701 accaagcgga aaaaagtaag ggttgttgat tttggagata cactggtaga gattttccgt
    32761 aatgcccgga aagagcagtt aaaaaatcga atgcagtacg gagaacttta tcacacgaac
    32821 tactacaaag aggtcaaaga gaaaaacaga gtgtactacg aatattactg cttagacaga
    32881 acagaggaag tcccagcaga ttataaagaa atttcttttg tttgcttaag accggatggt
    32941 tgcctggaac ttccgactac tttgggaacg gtatgcagaa aggtggcaaa aacattagag
    33001 ggatttgaag gctttcattt ccaccagtta cgtcacacct atacaagcaa ccttttagca
    33061 aatggagcag ccccaaaaga tgtgcaggaa ttgttgggac actcagatgt cagtaccaca
    33121 atgaacgtct atgctcactc cacaagagat gcgaaacgaa aatcggttcg gcttcttgat
    33181 aaagtggtgg gcaatgacta aaaaatttcc cttatttccc ttgtgattat ctcataaggg
    33241 caaaaataag ggaaactaca tatcatttca ctaatggaca
//
LOCUS       DAWEJW010000018        31388 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_613_length_31388_cov_60.939999, whole genome shotgun sequence.
ACCESSION   DAWEJW010000018 DAWEJW010000000
VERSION     DAWEJW010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 31388)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 31388)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..31388
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_613_length_31388_cov_60.939999"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         8789 a         6650 c         8794 g         7155 t
ORIGIN      
        1 atgaaggaac acattcagct tggaggatag ggaataaaga aaattacagc ttgtgatatt
       61 ccgggctgcg cttttcgtag attgtgtaat atttgaaacc gcacagctga aggatctcgg
      121 aggcaagatc cagataggat cccacatcct gaggtttgtg ggcatcggtt cctacggtga
      181 tgatctcacc gcccagctca tgatagcggc gcagaacatc ggtacaggga tgaggctgtc
      241 tgagaccgta gcggaagctt ccggtgttca gttccagacc ttttccctgt tgcactaaaa
      301 gctccaggat cggatcaatg gcatccttat atttgtcata ggaatagttc ttgtccttgg
      361 tgggagccac acggacgata tagtccagat gccccaatac atcataatcg tcatggcatt
      421 tcagattctc cagtgtctct gcaaaataag cccggatggc ttcttcctcg gaggaaaatt
      481 tctcgaaata ttccggataa tagggatcca ttcctttgca gagatgagtg gagccgatga
      541 cgaaatcaaa atcataggcg gaaataaaat cttcgttgcg gtcggccaga tgaggctgca
      601 atcccagctc cacgccgaag aggatccgca ttttcccttc atatttcgcg cggcagcgga
      661 gcacgtcgta cagataggaa tccgtattca gtaaaaagat cgttcccggt ccttcctcgg
      721 tgcggggata atcaaaatcg ttatgctcgg taaaacacat ggtttccagt cccagagcga
      781 gacctttatc gatcatctcc ggcatgggag cctgggaatc tcccgaaaaa gaagagtgaa
      841 gatgacaatc tgctttgata gacatatttt tcatcctttc tggttatcat aatacgaacc
      901 tattatagca aaaatgaagg gaaaaaccat agcatttcta aacaaactgt aaaatttacc
      961 acaaaatcac aaatattttt catttacatc ttgaattata atcaggaatt aggtaaaatg
     1021 tctttgctga taaaattttg acaatctgtg aataacggaa agccaaaaga tataaaatta
     1081 aaattttagg aggaaactaa aatggcagta agagtagcaa ttaatggttt tggccgtatt
     1141 ggtcgtcttg cattcagaca gatgttcgat gcagaaggat atgaagtagt agcaatcaac
     1201 gatttaacca gccctaagat gttggcacat ctgttgaagt atgattcttc tcagggtgtt
     1261 tacaagtatg caagcaccgt tgaggctggc gaggacagca tcaccgttaa cggcaagacc
     1321 atcaccatct acaaagaggc tgacgcaagc aagcttcctt ggggtgaatt aaaggttgat
     1381 gtagttctgg agtgtaccgg attctacacc tctaaggaga aggcttccgc acatatcact
     1441 gccggtgctc gtaaagttgt tatttctgct cccgcaggta acgaccttcc taccatcgta
     1501 tacaatgtta accacaagac cctgaagccc gaagataccg ttatctccgc agcttcctgc
     1561 accaccaact gtttagcacc tatggctaag gctctgaacg attgcagacc tatcatgagc
     1621 ggtatcatga ccactgttca cgcttatacc ggtgaccaga tgatccttga cggacctcag
     1681 agaaagggcg atctgagaag aagccgtgca ggcgcttgca acatcgttcc caactctacc
     1741 ggtgcagcta aggcaatcgg cctggtaatt cccgaactga acggcaagct gatcggctcc
     1801 gctcagcgtg ttccgacccc taccggttct accaccattc tggtagcagt agttaagggc
     1861 gctgtaacca aggaagagat caacgcagca atgaaggctg cttctaccga gtccttcggt
     1921 tacaacaccg atgagatcgt atcttccgat atcgtaggca gcacctacgg ttccatcttc
     1981 gatgcaactc agaccatggt tgctcctatg gaagatggca atactcaggt acaggttgta
     2041 tcctggtatg ataacgagaa cagctacacc tctcagatgg ttcgtaccat caagtacttc
     2101 agcgagttag cataagccga caggcgttaa cgcgtaaaga cctatcaggg tccggtctta
     2161 caaggaccgg gcccttttta gatactaaaa taattggagg ttaataccat gggattaaat
     2221 aagaaatctg tagatgacat taacgcaaag ggaaagagag tcctggtaag atgcgacttt
     2281 aacgttccct taaagaacgg tgagatcact gacgataccc gtatcgttgc agctctgcct
     2341 accatcaaga agctgatcaa cgatggcggc aaggtaattc tttgctctca tcttggtaag
     2401 gtaaagaatg gccccaacga aggcgaatct ctggcacctg ttgccaagag actgtccgag
     2461 aagctgggca aagaggttac tttcgtagct gactacaatg taaccggcga agcagctacc
     2521 aaggctgttg agaacatgaa ggaaggcgac gttgttctgc ttcagaatac ccgtttccgt
     2581 ggcgcagaag agaccaagaa cggtgaggaa ttcagcaaag aactggctga cctggctgat
     2641 ctgtatgtat gtgatgcatt cggctcttct cacagagcac atgcttccgt agcaggtgtt
     2701 accaagttca tcaccgctaa gggtggtcag aacgtagtag gttacctgat ggagaaggag
     2761 atcaacttct taggtaaggc tgtagaagat cccgtaagac ctttcgtagc tattctgggc
     2821 ggtgcaaagg ttgcagacaa gctgaacgtt atctccaacc tgttagagaa gtgtgatacc
     2881 ctgatcatcg gtggtggtat ggcttacacc ttcttaaagg caaagggata tgaaatcggt
     2941 aagtctctgg tagatgatac caagatcgac tactgtaagg aaatgatgga aaaggctgag
     3001 aagctgggca agaagctgtt actgcctgtt gattccgtaa ccatcgctga tttccctaac
     3061 ccgatcgatg ctcctgttga ggttcaggta tatgatgtta ccaacatgcc cgcagacaga
     3121 gagggatgcg atatcggacc caagaccgca gctctgtatg ctgaggctgt taagactgct
     3181 aagaccgttg tatggaacgg acctatgggc gtattcgaga atcctaccct ggcagcaggt
     3241 accatcgctg tagctaaggc tctggctgag accgatgcaa ctaccattat cggtggtggt
     3301 gactctgcag cagctgttaa ccagcttggt ttcgctgaca agatgagcca catttctacc
     3361 ggtggcggag cttccctgga gttcctggaa ggcaaagagc ttcccggcgt ggcagcagca
     3421 gacgacaaat aaatagaaat aataaagctt tcccacggaa tgtattcgaa cctgcctgca
     3481 ggcacagatg catcctgtgg gaaacacaat ataagaaaag aagaggtgta tgattatggc
     3541 aagaaagaag atcattgccg gcaactggaa gatgaatatg actcccagcc aggcagtaga
     3601 gctgatcaat acattaaagc ccctcgtagc aaatgatgaa gtagacgtac ttctgtgcgt
     3661 tcccgcaatc gacatcattc ccgctatgga ggcagcaaag ggttccaata tcatgattgg
     3721 tgcggagaat atgtatttcg aggagaaggg tgctttcacc ggcgagattt ctcctgcaat
     3781 gctggatgat gcaggcgtga agtatgtgat catcggacac tccgagagaa gagagtactt
     3841 tgcagagacc gacgagaccg tgaataagaa ggttctcaag gctttcgagc atggcattac
     3901 tcccatcatc tgctgtggtg agactctgac gcagagagag cagggtgtta ccatcgactt
     3961 catccgtcag cagatcaaga tcgcattcct gaacgtaact gctgcacagg cagcaacggc
     4021 tgtcatcgca tacgagccta tctgggctat cggtactggc aaggtagcta ctaccgagca
     4081 ggcacaggaa gtatgtaagg caatccgtga gtgcatcgct gagatctatg atgatgcaac
     4141 cgcagcagct atccgtatcc agtacggcgg atccgtatct gcttccagcg ctcccgagct
     4201 gtttgcacag gcagacatcg acggtggtct ggtaggcggc gcttccctca aggctgactt
     4261 cggacagatc gtaaactata agtaatagaa caattgcaca aaaaataggg gcttaaaacc
     4321 cttgcaaaaa gaggattctt tgttgaaagc caacaatgga atcctctttt gttatgttat
     4381 ataatttaat aagtatcacc tttttctatc actaaactat catcaggaat gatgggttat
     4441 gtcattttct tacgtagatt atgacagata gattaaaatg aatgtggttg gtcgggtgtt
     4501 tataaggtat aattttatat tatagcatga ctcgcttttg gctatggaat aaggctatga
     4561 tttttgtttt aggttttgaa ggtctgagac gcatggaaag tcaggaaaca ctatatgaaa
     4621 tatgtattta ccaatggaaa aatcttaaac ggtacaaaag acatgcaggt acaggagggg
     4681 caggttattc tggtggaaaa tgggaggatt acagagcttc tgccagcgga agaagccggg
     4741 aaacgaaatc tgaaggcttc cggatatgaa gaaatcgact tacagggaaa atatatcctg
     4801 ccggggctga tcaacatgca tgtccatctg gcaggcgacg gaaagcccca gaagaaacag
     4861 cgtgacaatg aagcactggt caaaaagatc atgagcaatg gcctgacaaa ggctatcgcc
     4921 tacaatatgg tctgcggttt tgcaaaagac gaactgtaca gcggtgtgac tacgatccgt
     4981 actgtgggtg gactgggaga ttttgacacc agactgcggg acgacattgc cgctggtaaa
     5041 aagcccggcc ccagaattct ggctgccaat gaaggaatct ctgtgcccgg cggacatatg
     5101 gcaggctctg tagctatcgc cgcagacagc gtggaagaag cgctgcagca cttagagact
     5161 tcgaaggcac aaaaggtgga tctggtgaaa cttatgatca ccggcggcgt gctggatgcg
     5221 aaggaaaagg gtgtgcccgg cgaactgaag atggcccctg aaatggtaaa ggcagtctgt
     5281 gacaaggccc acaccatggg gtatatggtg gcagcccacg tggaatctcc ggagggggta
     5341 aaggcagctc tgaaaaacgg ggtggattcc attgaacatg gtgcaaaagc agatgaagaa
     5401 atgatttcac ttttcaagga acataatgca tttttgtgta ccacattgtc tccggcactt
     5461 ccttatgcat tgtttgaccg atccattacc aatgcctctg aagtggagca gtttaatggc
     5521 aatgttgtat ttgaaggtat tatcgactgt gcgaaggcgg ccattgccaa tgatattccg
     5581 gtggtgctgg gcaatgatgt gggatgcccc tggatcaccc agtacgattt ctggcgggag
     5641 ctgtactatt tccacaagta tgtgggagta tccaacgctt ttgcactgta taccgcaacc
     5701 tgccgcggcg ccgagatggc gggtatcgga gatatcaccg gtacactgga atccggcaaa
     5761 tgcgcggata tgatcgtggt ggagaagaat ccgttagaag atctacgggt gcttcgaaat
     5821 gtggatatgg tcatcgtaca gggaaaagtg atccgtgctc ccaaggtgaa gaaaaagcag
     5881 atcgtagaga cggagttgga caaatttctg aattagaatg gaacggcaat gtctgtttac
     5941 gtagaataga attgtattct gatgcgtgaa tattgtataa taaaaactat gcaggacagg
     6001 aaacctccgc tgcatagttt ttatttttga cagattatgg gaaattatag gaaaaagaaa
     6061 atgacatttg aaaaattaga aaaaaacctc atggatgtgg ttctggagga acaggcaaaa
     6121 ttaggcttcc gcagagagaa gatccgcctg tattatccgt tatctacact gaatcacttg
     6181 ctgggtacac aggatacggc ggaacagatg gaagttacac tggcaggaca gccggagagc
     6241 atgacaataa agcttggaaa actggctgtg agccgtaagg gagaccgttt ctgcctctgt
     6301 attccggagg aaggcagtgc ttatgtccat gagcattttg cagagaaggg atttatctat
     6361 gaactgatcc gcttgatcgg gcagcatgac tgtaagatgg aggatatccg acagttattt
     6421 ttgcggtaca gtgagaatat ttatgtggaa gagatgcggg gcgaggactt tgatgtcatg
     6481 atccgtttcc cggaggaaat gggagatccc tactgttatt gttttcggga tgaagggtgc
     6541 catgtgatct atcaccgatt cctgccggag gattacgcag acctgatgaa agcatgagac
     6601 aatcatggta aacgaacgat aagaaaatat tatgtgtatt ttacgatatg gaaatacgat
     6661 ggaatctatg gtaagataaa aaaggttcta cgaagaaaaa tgtcgtaaaa ggcagaaggc
     6721 gaggggtaaa atgatcttta gatgtaaaaa ctgcggcgga aatgtagttt acagcccgga
     6781 gagaaagaag atgttctgtc ctttctgtga gagtgaggac agcgaagaac gcgaggacgg
     6841 cgtgatcgag acaagcggtg aggaaaacgg cactgcggaa ggaacggtcc aggcagcggg
     6901 agccggacag ccggtcagca gaatggtagc agccgagggt gctgcgcaga aaaaggtgac
     6961 aaaggtatgt cccaactgtg gtggcgagat tcccatgacg gtaaacacct ccgctacaca
     7021 gtgtccttat tgcgacaatt atgtgatcgt ggatgatcag atttccgggg cctacacacc
     7081 gcatatgctg attcccttcc gtatgggcaa ggaagtctgc aagaagctga tccgtgataa
     7141 atttgagaaa tgtatttttg caccgacgga ttttctgtcg gaagtaagaa tgaacggtat
     7201 ttacggtgat tatgtaccgt tctggttcta cgattacaat accaactgca cgttccacgg
     7261 tgaaggtacc aaggtgcgca gctggacgac cggcaataca cagtataccg agacctcata
     7321 ttatgatatt gtccgtgata tggacattga ttttgtgaag attcctgtgg atgcttccgt
     7381 gggaatgccg gatgatgtca tggatctgat ggagcccttt gattacaagg aactgcagga
     7441 gtttaagcca gaatatctgt ctggatttca tagtgaacgc tacaatatga cttccgatct
     7501 ggtggagagc cgtgccaaag ccaagatgca ggaggatgca cagaagatgc tgcatgacag
     7561 ctattctgga tattccagtg tccgcaaaat gggagaaaac atcaacgtga cggacagttc
     7621 tgtaagttat ggtctcctgc cagtatggaa gtatgactat agctacaaag gcaaagaata
     7681 tcctttctat gtaaacggac agaccggtaa actggtaggt actgcaccga tttccaaagc
     7741 aaaggtgtgg gcttatgcgg gaacacttgg tgtgacactg gcggcaattc tatgcatggt
     7801 gaatgcaatc gccagtctgg tgtagaaatt atgagggaag gataacggga caatggaaaa
     7861 agatattaag aaagaagcac tgagacagta cctgaaatat tttaaggtat ggttcatcat
     7921 tgccggcata ttggcggtga tcaccatagg agcggcagtc gttcatctgc ttacacccaa
     7981 gactgtccgg ggcaacagcc aggatcccac agagcgtgtg tttgactatg cggatatgct
     8041 gacagacgaa gaggagcaga gcctgcggga atatatagca gagtgtgagg aaaagatcca
     8101 ggcggacatc gtgatcgtaa cgatctccga atccgtagag tatgatctgc agaatccggg
     8161 tcttgcggag gcatttcatg cgaaggaagt cggcagtacc gactggagta cagccatgag
     8221 agatctggcg gataacttct atgattacaa caatttcggt tatgacaagg tacacggcaa
     8281 cggagttctg ctgctggaca atgcctatga aggacaaaag ggaagctggc tgtctacctg
     8341 tggaaatgtc tatgattatt ttggcgatta cgagatcgac caggccctgt acgcagtgga
     8401 tgattacata gacgaaagcc cctacaaagc gtataagaat tgtatcagtt atgtgacccg
     8461 gaccatggag gaaagtaagg aaagcatgcc gatgaccttt tctccctgga tcctgacagg
     8521 tcttgtcgtt gcactgatct atgccgcagt gaacctgcat cagagaaaag caaaggatac
     8581 tactacggtc aatcagtatc ttgatggcaa aaagcccaag atcaacaata ccagagacca
     8641 gtatctgcgt aagaacgtgg tgacccgcag gatcgagacc agcagtagca gtagcggaca
     8701 cagcagcgga cacagcggcg gtcacggagg aagtcatcgg agcagcagcg gtgtcagcca
     8761 tggcggcggt ggccatagaa gataaaataa caagttccat tcctggaaca agagatccgg
     8821 cgtatcgggt gcagacagac actcatacgc cggttttttc gttttacgga ggaaggagcg
     8881 acggcgggaa acagtagtgg aaaatttttc catgggttga caggaagcct gcagacaggt
     8941 ataatcagac ttgcgatgta ataattctgt aatatatttg taaaaggatt ttaaagaaga
     9001 atttatgata aaacatacaa gagaaaaaca agtaaagatc gtagccactg gcctggcatt
     9061 gtgtatgctg gcggcaccgc tttccgcttc cgctgcagaa agcagtgtcc tcatggcatc
     9121 cggcggtgtg gcttctgtcc tggagacgga gcgtacactg gaggaataca tccagatcgc
     9181 acaggatgcc cagggagctt cctggggcta taccaatatc ggtgtggcca atgtggaatc
     9241 cggtaatctg aatgtgagag cggtatcttc cacagacggt aagctggtag gtaagcttcc
     9301 caaggatgcg gcctgtgaag tggtcgagac cgtagatgac tgggcacata tcaagtccgg
     9361 tgaagtggaa ggttatgtca gcaaggaatt cctgctgact ggaccggaag ccagaatccg
     9421 agccatggaa ctggttcata cggtagccat tgccaatacc gatggcctga atgtacgtca
     9481 ggagcccaat accaccagtg agatcgtgac tcaggtggga cagggagaag agatggagta
     9541 tgtggagacc ggttccgacg gctgggtgaa gatttctatt gacggcgagg atgcttatgt
     9601 atcccaagat tatgtcacag tggaagaaaa gctggatacc gccatcacca tgacagagct
     9661 tctctacgga cagggggtat ccgatgtccg cgtagatctg gtagaatatg caaaacagtt
     9721 cgtaggtaac ccttatgtct ggggaggcac cagccttacc aagggtgcgg actgctccgg
     9781 ttttgttctc agcgtattca agaaatacgg cattaccctg agccattcct ccagagcaca
     9841 ggccaatgag ggcaccaaga tcagtgcatc cgagctaaag cccggtgatc tggtattcta
     9901 tggtaacggc aagggtaata ttaatcacgt agctatttat atcggtggag gacaggtaat
     9961 tcacgccagc agcccgaaga ccggtatcaa gatcagcagc tataagtatc gtacacctgt
    10021 gaaatgtgtc cgtgtcattc acgactgata aaaagcattg ctttcgcaaa agggcggggc
    10081 aatgcttttt tgtgtacaaa tcgagtagga gacagcctgc ttgattattc gaagatatgc
    10141 gcaggatctt tcttttacaa ttgcgtttcc cagggaagta tgatactatt tttgtataca
    10201 tcagcggtcc ctgacaggaa aaaagggggc ttcgtacaga gacgaaaatg ggtgaggaat
    10261 atgcggaaag tagctttgat caccgatggc tggagaaggt tgtttaccta tgcctggccg
    10321 gcaggtattt tgcagagaat caaagagagg gacgaagaag taaatttata tatttttaac
    10381 agtactggca actggagcag agatgctggg tacaacaggg cagaatataa tatttttaac
    10441 cttccggatc tgtcagagtt cgacggcatt attctggaat tgaacaatat cagctctccg
    10501 gcagtgctgg cggaagtgat ccacaaggcg aagcagtcgc accttccggt agtctctatt
    10561 gccaacgagc tggaagattt ttattatgtg ggtattgaca attattctgc catgaagcag
    10621 gtgatcgcac atatgcatga ggtgcataac tgcaaaaaat tctggctgct ggtgggggct
    10681 gacagcaatt atgagagcag ctgccgactg cagggaatgc ttgactatgc caaagaacac
    10741 aagatcaaaa tagataagaa tgacatctgg tacggctctt ttgactacaa gagcggattg
    10801 gaaggctaca ggcatctgtg ggatactcac aaagagctgc cggatgctat tatctgtatc
    10861 aatgataatg tggcggtagc ggtatgcgaa gcggcggccc agatgggatt tacagcaccg
    10921 aaggacttcc gtatcacagg ctttgacaac ttcgacaagg ccggatttta cacgccgagt
    10981 attaccactg tgggacatat cagagaggaa gctgcataca agggaatgga tattctcctg
    11041 aaaatctggg cgggagaaag tgtcccccgg tacaatttca ccaatactga gatgctgtgg
    11101 caggagagct gtggctgcgg caaaggaagc agcagggatg ccagagccca tctgaaggat
    11161 cagatcatgt acagcgtgga aagtgaggaa ttcgacgagg aagtcctgtc catggaggca
    11221 gagatgatgc agtgcaatac cgtagaggaa atgatgtact gcattcccca gtgcattccg
    11281 tccctgaaat gcgatgccat gtatctggtg ctggacgatc acctcaatgc ctataaaaaa
    11341 gaaacggaga agagtattca tctggatgcc accccttcgg acgagggatt ttccttgcat
    11401 ggttatccga gacagatgca gatgaccttt gcatacgaga gggaccagag actggatctg
    11461 gacagacagg aagtggatgg catcttcccg accttcgatt atccgaagcc gggacaggac
    11521 tttttgttcc tgccgttaca tttcggagag cgtaccgttg gctatgtagc tatccgtaat
    11581 gcggtatacc tgatggagaa acagtacctg ttccagatca tgaatgcact gacccgctcc
    11641 atggagaacc ttcacaaaaa agaaatgttg gcctatatga accgcaagct gtcttccctg
    11701 tatattatgg atacgctgac aggtatgtat aaccgtatgg gctaccaaca gctgggagag
    11761 gagtccttcc gtatttccgg tctgaacaaa cgcaaacttc tgatcttgtt cgtggatctg
    11821 gatcgtctga aatacatcaa tgacaccttc ggacacgagt atggggatat ggccatctgc
    11881 gctgcagcca gagcattgat gcagtgtagc agccgggacg ctgtgccggc cagaaccggc
    11941 ggtgatgaat tcgtcctgat ccagagttac cagtccgatg aggtttccag agaactggtg
    12001 ctgcgcatcc gcagaacact ggaggcggag ggaaaagccc agaagcttcc gttcccgttg
    12061 tccatgagta tcggaactgt cgtgacggat ccggaatccg cactgtcttt tgcagattat
    12121 gtaaaaattg cagacagccg gatgtacgag gaaaaggtac agaagcgggc cgcaagaaaa
    12181 tagaaaaata tatttacaac cgatagaaac tatgatatat tgaaatagta tgattagccg
    12241 atgcccagat tacggacact ccgaccttag tcttggtatc tggcgtttaa aaaacaggag
    12301 gaaaaagaaa tgatttctaa ggaaaagaaa acagccatca tgaacgagta tgcaagaacc
    12361 cccggtgaca ccggttcacc tgaggtacag gtagcagtcc tgaccgcaag aatccaggag
    12421 cttaccgagc atctgaagga gaaccccaag gatcatcatt ctcgtcgtgg tatgctgaag
    12481 atggtaggtc agagacgtgg tcttctggag tacctgaaga agaaggatat tgagagatat
    12541 cgtgcattaa tcgagaagct tggcttaaga aaataatgct gactggtgag cggggtctgc
    12601 cgattacatc ggcgctccgc ttctcttgtt tctcattaca tccatatgca cggtgaaaat
    12661 ctaattcacc gcgcctgaaa aggtaacaca cgggatggaa gtgagccccg agaccgctga
    12721 aaagattatg ttgcggatca tgggagcaac atggaatttt cagtagtttc ggtaatcctc
    12781 cagcccgtag aataataatt catatcgtag cgtggcacca aagataacag tcacagtacg
    12841 atggatagga ggaatttatg tatcaaagtt acaagatgga tcttgcaggc agacctctgg
    12901 tggttgatat caaccgtgta ggtaagcagg caaacggatg tgcattcatg cactatggtg
    12961 ataccactgt tctctgtacc gcaaccgctt ccgagaagcc cagagacggt atcgatttct
    13021 tccctctgag tgtagagtat gaagagaaga tgtacgctgt gggtaagatc cccggcggtt
    13081 tcaacaagag agaaggaaag gcaagtgaga atgctattct gaccagccgt gtcatcgaca
    13141 gacccatgcg tcccctgttt cccaaggatt accgtaatga tgtaaccctg aacaacatgg
    13201 taatgagcgt ggatcccgag tgtcgtccgg agttggtggc tatgatcggt gcagctatcg
    13261 ctacctgcat ttccgatatt cctttcgatg gcccctgcgc tatgacccag gtaggcatga
    13321 tcgatggcga gttcatcatc aatcccagcc aggagcagtg gaagaaaggt gatctgaacc
    13381 tgaccgttgc ttccaccaga gaaaaggtga tcatgatcga ggcaggagcg aacgagattc
    13441 ctgaagcaac catgatcgaa gcaatttata aggctcacga agtgaaccag accatcattg
    13501 ctttcatcga caagattgta gcagaagtag gcaagaagaa gcatgagtac accagctgtg
    13561 cagtgcccgc tgaaatgttc gaggagatga agaagatcgt atctcccgct gagatggaag
    13621 aagctgtatt caccgatgat aagcagaccc gtgaggagaa tatccgtgta atcaccgaca
    13681 agctgaccga ggctttcgct gagaacgaag agtggctggc tatcttaggt gaggcagtat
    13741 accagtatga gaagaagacc gttcgtaaga tgatcctgaa agatcacaag cgtcccgatg
    13801 gacgtgcaat cacacagatc cgtcctctgg cagcagaagt agacatcatc ccccgtgtac
    13861 atggttctgc tatgttcacc cgtggacaga cccagatctg taacgtatgt actctggcac
    13921 ctctgtccga gcagcagaag atcgatggcc tggatgagaa cgaagtaagc aagagatata
    13981 tgcatcacta taacttccct tcctattccg taggtgagac caagccttcc cgcggacccg
    14041 gaagacgtga gatcggtcac ggcgctctgg ctgagagagc cctgatcccc gtactgccca
    14101 gtgaggacga gttcccttat gcaattcgta ccgtatctga gacttttgaa tccaacggat
    14161 ctacctccat ggcatccacc tgtgcttcct gcatgtccct gatggcagca ggcgtaccca
    14221 tcaagaagat ggtagcaggt atttcctgtg gtctggtaac cggcgagacc gatgacgatt
    14281 tcgttctgct gaccgatatc cagggactgg aagacttctt cggagatatg gactttaagg
    14341 taaccggtac taccgaaggt attaccgcga tccagatgga tattaagatc cacggtctga
    14401 ccagacctat cgtagaaggc gctattgcga gatgccgtga tgcaagactg ttcatcatgg
    14461 atacctgcat gaagcccgct atctctgctc ccagaacaga ggttggcaag tacgctccca
    14521 agatcatctc cattcagatc gatcccgaca gaatcggtga tgtagtagga cagcgtggta
    14581 agaccatcaa tgagatcatt gcccgtaccg gtgtgaagat cgatatcacc gatgatggta
    14641 aggtatctat ctgcggtact gacaaggaaa tgatggataa ggctctggag atggtgaaga
    14701 tcatcaccac cgatttcgaa gagggccaga tcttcaaggg taaggtagtc agcatcaagg
    14761 agttcggtgc attcatcgag ttcgcacccg gcaaggaagg catggtacac atctccaaga
    14821 tcgccaagga gagaatcaac cgtgtagaag atgttctgac tctgggcgat gtagtgactg
    14881 tagtatgcct gggtaaggat aagatgggca gaatcagctt ctccatgaag gatgtagcac
    14941 agcagtaagc tgtaagaacg caaggcataa gaatataaga tataaaataa gaaaggatcg
    15001 ctgcatagtg tactgtgcag aggtcctttt tgtgtatacg atgagccagg tacagatttg
    15061 tgcttgcaca agaatcggta tttggcaaat gtacatcatc gagcttttaa gcgagatggt
    15121 gtataaagta tatgaaacgg gcggaagaca gtcagccgga tctgcctgga atgaggcagc
    15181 aagaagtgac atttgaaaaa gggcacaaaa aaagcaccta catgacggta taggcgcttt
    15241 tgtatgtctt aaattctgta ccagtgcaag aaaaattaag ccattccgtt gacagctttg
    15301 ctcagacggg aaacctttct tgcagcgttg ttcttgtggt aaacgccctt agaagtagcc
    15361 atctcgatgg tcttggtagc agcggttaat tcagccttag ctgcttcctt gtcaccggtc
    15421 tcgattgcag cgtatacctt cttgatagcg gtcttaacgc cggatctgat agctttgttt
    15481 ctgtcagctc ttgtgcggga tactaaaatt ctcttttttg cagatttaat gttagccaag
    15541 tcgtacacct ccataatgta tacaataaaa ttataattta caagaaatgt ttcactttag
    15601 ccagacacgg aagactaacg taaacatact attgtagttt agacaatctg ggagcttttg
    15661 tcaatacata ttttgcggat ttttgtaaaa aatgtattga taagaaaggc agacagaggg
    15721 tgaaaaaatg gaaacttttc agatcagaac agacctggcc ctggaggcaa gagaaagtgt
    15781 caatgaagag gaaagcaaac tgcggggtgt gtcggtggac gagcactatg aggaagagac
    15841 ggatatccgc attaccaagg tgaccatcga tacgaaaaat gcggaaaaaa cactgggaaa
    15901 gcccatgggt gtctatgtga ccatggaggc accggccatg gtggaaccgg atgacgatta
    15961 ccacagggag atctcggagg cgttagcgga ggaacttttg aaaatgatgc ccggggaagc
    16021 ggaggaactg tcggtattga tcgtggggct gggaaaccgg gaagtgacgg cagacgctct
    16081 gggaccgcag gtggtggaca atctgctgat caccagacac gtggtcagag cctacggcaa
    16141 agcagcctac aactgtaccc gtatgaatct ggtcagcagt attgagccgg gggtaatggc
    16201 gaagaccggt atggagacgg cagaaatcat aagcggtgtg gtgaaggaga cgcaaccgga
    16261 tgtgctgatc gtggtggatg cgctggcggc tcgcagcacc agaagattga accgtacgat
    16321 ccagatcacc aataccggca ttcagcccgg ctccggcgtg ggcaatcacc gcaatgccct
    16381 gaccgaagag accctgggcg taccggtaat tgccatcggg atccccacgg tggtggatgc
    16441 cgccacgatt gtgggagatg cactggagaa gctgatgagc ggggaaaagg agttcgatgc
    16501 ggtcaaatat atgggacagc accgcatggc ttttgcagaa ctgaacaata tgtatatgac
    16561 cggaaaggat atcgattctg tgatcaaacg ggtcagctac acggtctccg agggaatcaa
    16621 tattgccatg gaaaaaaact gggcgtaact gcatatatat agggtaacag ttcatgagtt
    16681 gagcaccgga ttacgagcaa aggtagtcac gaagtgacgt aagagcaggt caaggcgact
    16741 ttgtgactga cgagggctga actgtcgtaa caaaaaaagt gcggaaggag agcgcggacc
    16801 gtgaagaatc cgaaaaatac tgtctggcgg atcgcactgt ttttgctgac agttatctgt
    16861 gggcttttga tggcggatcg gggagaaaat gagtttccgg tatttgcctg ttccgaagct
    16921 ttgtcaggag aaagcggcag taccggatta ctgctgcagc agaatatgtg ggaagtagta
    16981 ccgctgttag gctatggtat tgtggagcag caggggacac agaatccgga ggaggatgag
    17041 actacctgcc ggaccctgac caccgaagat ctgatcagcg aagaacttct ccttccgcag
    17101 ccggaggaaa cgcagcagac ggcccttatc ccggagagtg tggaggcaac agccgggact
    17161 gcattgagca attttcgtcc ggcggtacaa caggtgcaga ttgaccgcac acagcttgcg
    17221 gattatgaga cactggtacg gaatttttat gccatagatg ccaataccat ggcaggcagc
    17281 gatcagttat ccgtggaaaa actgctggga atggatatga cgttgccaca ggaaggtgac
    17341 ggaccacaga tcctaatcta ccacacccat tcccaggagg cttttgcgga ttcggttccg
    17401 ggagatgtga acaccggtat cgtgggagtg ggagagtgcc tgaccaagat cctgacagaa
    17461 caatatggtt accgggtgct gcacaatacc ggtcaatatg atgtggagac cagagacaat
    17521 gcgtattccc gggcacttcc ggcagtggaa cagatccttg cggagaatcc ctctatccag
    17581 gtgatcatcg atctgcaccg ggatgaggtg gcggaagaga caaagctggt gacggatatt
    17641 caagggagac ccacggcaag atttatgttc tttaacggtc tgagcaggac ccgcaagacg
    17701 ggagatattg attacctggc caacgagaac caggaggcca atctggcatt ttcttttcag
    17761 atgcagttga aggcggcgga gtattacccg ggactgaccc gcaggatcta tctgaagggc
    17821 taccgctaca atatgcatct gcgtccccgg acacttctgg tggaactggg agcccagaac
    17881 aatactgtgg aggaggccat aaatgcctgt gatcctctgg cacatattct ggatatggtg
    17941 cttaagggag agtagcgata cataaaagca ggataagaca acgaatcgaa gtaaaatcat
    18001 agacaagaat gcggaattta tgctacaatc gttagatgca agaatatgtc aggaaaggac
    18061 agtagtatgg cagtaataga tcagagcaaa atccgcaatt tttgtattgt ggcacacata
    18121 gatcacggca aatccaccct ggcggaccgt atcattgaga agaccggtct gctcaccagc
    18181 agagagatgc aggcacaggt cctggacaat atggacctgg agagagagcg tggcattacc
    18241 atcaaggcac aggccgtgcg taccgtatat aaagcacagg acggagagga atacattttc
    18301 aacctcatcg atactccggg ccatgtggat ttcaattatg aggtcagccg ttctctggcg
    18361 gcctgcgacg gcgccattct ggtggtggat gcggcacagg gcattgaggc acagacactg
    18421 gccaatgtct atctggcact ggatcatgat ctggatgttt tcccggtcat caacaagatc
    18481 gatctgccca gcgcagatcc ccagagagtc attgaagaga tcgaggacgt catcggtatc
    18541 gaggcacagg atgctccgct gatctccgcg aaaaacggca tcggtattga ggaagtctta
    18601 gagcagatcg tgaaaaagat cccatccccc aaggggaatc cggacaaccc tttacaggca
    18661 ctgatctttg actctgtata tgattcctat aagggtgtta tcattttctg ccgtgtcatg
    18721 gaaggcacag tgaaaaaggg taccatgatc cgcatgatgg ctaccggagc gaaggaagag
    18781 gtcgtggagg tcggttattt cggagcggga cagtttatcc cctgtgacga gctttctgcc
    18841 ggaatggtag gctacatcac ggcttccatc aagaatgtaa aagatacagc agtgggtgat
    18901 accgtaacgg acgacaacaa tccctgcgca gaacctctgc ccggttataa aaaggtacag
    18961 tccatggtat attgtggatt atatcccgca gacggttcca agtacccgga tctgagagat
    19021 gcgctggaga agttacagtt gaacgatgcg tccctgttct atgaaccgga gacttctgtg
    19081 gcactgggat ttggcttccg gtgcggattt ctgggactgc tgcatctgga gatcattcag
    19141 gaacgactgg agagagaata caatctggat ctggtgacta ccgcccccgg cgttgtctat
    19201 aaggtgcata agaccaatgg ggaagtaatt gagttgacca atccttccaa tctgccggat
    19261 ccctccgaga tcgaatatat ggaagaaccg gtggtaaatg cagagatcat ggtgacttcc
    19321 gaatttatcg gttccatcat ggaactgtgt caggagcgcc gcggcaaata cctcggcatg
    19381 gaatacatcg agggcaccag agcgttgttg aaatacgaac tgcccctgaa cgagatcatt
    19441 tatgatttct ttgatgcttt aaaatcccgt tcccgtggtt atgcatcttt tgattacgat
    19501 ctgaagggtt acgagagatc gaatctggtg aagctggaca ttctgatcaa ccgcgaggaa
    19561 gtggatgccc tgtccttcat cgtctgtgca gattccgctt acgagagagg acgtaggatg
    19621 tgtgaaaagc tgaaggacga gattcccaga cacctcttcg agattcctat ccaggcagcc
    19681 atcggcggca aggtcattgc cagagaaacg gtaaaggcca tgcgtaagga tgtgctggcg
    19741 aaatgttacg gcggtgatat tacccgtaag aagaagctgt tggagaagca gaaagagggt
    19801 aaaaagcgta tgcgtcagtt cggcaatgta gagatcccgc agaaggcatt tatgagtgtt
    19861 ctgaagctgg atgataaaaa ctgatgaagt aattgaagga gcaatatgaa taaaaaggaa
    19921 ctggaattat acattcatat tcccttttgc gtaagaaagt gcagctattg tgatttcctg
    19981 tctgcccctg ccacggagca gacaagggaa gcctatatgg ctgcactttt tgcagagatc
    20041 gggggaagag caaaagatta cagtgaccgt accgtgacca gtatatttat cggaggaggt
    20101 acgccgtcgc tgttgtccgg ggaacagatt gggcagttga tggaccgtat cagagagcag
    20161 ttcgcaatgg cacaggacgc ggagatcacc atggaggtga atccggggac ggccagcgca
    20221 gagaaacttc ggaattttta cacggcagga atcaaccgtc tcagtatcgg tatgcagtct
    20281 gcccaggcag aagaactgaa aaatctgggc aggatccatg attttgaagg tttctgccag
    20341 gtgtaccggg aggccgtgga ggcaggattt acgaatatca atgtggatat catgagcgga
    20401 ctgccgggac agacgcttgc aagttatcgg gacacgctgg aaaaggtgct gcacttagaa
    20461 cccatgccgc agcatatctc tgcctacagc ctgatcgtgg aggagggaac cccttttgcg
    20521 gccatggcag agaggggaga attgcccttg ccggaggaag agacggagcg tgccatgtat
    20581 gaggagacca tagaggtgct ggcaaagtac gggttccatc ggtatgagat ttccaactat
    20641 gccagggatg gttatgaatg cagacataat gtgggctatt ggatccgcag ggattacctg
    20701 ggattcggta tcggtgcggc atcccttatc gataatgtgc ggtttcagaa cgaaaagagt
    20761 ctgggagctt atctggcaca tccgctggaa agcagggaag aggagcagac actgacggta
    20821 caggaccgga tggaggagac catgtttctg ggactgcggc tgacaagagg agtatcctgt
    20881 gtggattttg cagaacaata cggcaggact gtggaggaag tctacggaga agtgatcgcc
    20941 cggaatgtgg cggatggtct tttgaccgtt acggagagga aagagaatca gaaaaaagac
    21001 cggctcctgg cactgacaaa gagagggctg gatctgagca attacgtgat ggcgcagttt
    21061 ttattctgag gaacgtggga gactgacagg cacttgtgga cagaaaatca cataaactat
    21121 cataagcaac tgtccacgga gggatttttt gaagaccttt cagcagccat taaccgcagg
    21181 agaagaagcg tattatatcc gcttgctcag ggaaggtacc gtggaagaat ccagacaggc
    21241 cagggagatc ctggtggaga ggaatctgcg cctggtggca cacattgcca aaaaatacca
    21301 gaatgttgag gaagacatgg aggacctgat ctctatcgga tgtatcggac tgatcaaagc
    21361 agtgaattct tttgactcag gaaaaggaag actggctacc tatgcctgca gatgcattga
    21421 taacgaactt ctgatgatgc tgcgcagccg caaaaaactt tccagggaag tctccctgta
    21481 tgaacctatc ggacaggata aggaaggaaa tgccatccat ctgttggacg tgatcgaaga
    21541 gaagcagaag gacgtggtgg aggatctgga gcttgacaga aatatcaggc gcctgttcct
    21601 ggctctggac cactgtctta cggagcggga gtatcggatt ctggtgatgc gctatgggat
    21661 cctgggctgt aaagaacaca cacagcagga ggtgggagat gttctcggaa tttccagaag
    21721 ctatgtctca cgcatggaaa aaagagcatt gcataagctg gcggaggctc tgcgtgagga
    21781 ctaaacatta ccttgtcaat taaggaacac tgtgatatat ttatttctat aagtgactaa
    21841 agataagggg ggggacaggg atgcattttg taagaacgga agacttgaag acgggaatgc
    21901 gtctggcaag acctgtttat aataagaaga gtattctttt gtttgacaga aattcccttt
    21961 tgtcattaca ggcaatagaa agtattcgta atttcggatt gatcggtgtc tatgtactgg
    22021 aaccggcaga accgcttcca cccctgactc aggaggacct ggagttcgaa cggtttcaga
    22081 ttcaggcggt cagtgccatc gaagaggaac aggatcgtat cctgaagacc agaaagcaga
    22141 accgtaccca gtccatcgca gatatggtca tccgcaatta cggacacttg gatgagaaaa
    22201 tcaatttcta ccagaatctg agaagccggg aggattattt ctccagacat tccctgaacg
    22261 tggcaatact ctgtgccatg attacccatg tgatgaatat cagacgggaa gaacagtatc
    22321 ataccgtatg tgctgccatc cttcacgata tgggaaaagt gaaaaaacat gatgatgtgt
    22381 attacggggc accttatacc agagaggata tgctgcggaa ctgcgagacg caggaagagg
    22441 cctacggtat catcgaggag gcttttgcca ccgacggatc cggcatccgc aggatctgcc
    22501 agcaggcagc cagaaaacag ctggacctgt tcccggaaga cggagagaag agcaggtata
    22561 aaatgctggc cggagccaat gtattgctgg tggcgaaccg ttatgatgag atcacggcca
    22621 tgacattaca gggaacggca cagtctgagg tgaaggctct tcaggaactt ctggagcacc
    22681 cggaggtata tgatccggag acggtacggg cattgatccg ttccatcaat atccttccgc
    22741 ccggtgccag cgtggaactc agtacgggag agaaagcact ggtattgaat gaaaatgaag
    22801 aaagtgtact gcggccgaca gtggtaagct tccgggataa cagtatcctg gatctgtccc
    22861 ttcccgggaa tgaggacatc cagatcgtgg atgtcatgaa gaccatggac aaccggtaca
    22921 tattcaataa atagtgagga gcaattatgc caacaatcga agaaatactc agagccgcca
    22981 aggaagcggg agcaagtgat gtacatctga cggtaggtat cccacccaaa atgcgtgtaa
    23041 acggcaatct gatcaccatg gattatccca ggatgcttcc ggcagacacg ctggaagtac
    23101 tagtcaatat catgacggaa gtgcagagag aacgttttga ggaacgggga gaatacgata
    23161 tgtctttctc tattcccaac tgcggacgtt accgtgtcaa tgcctacaag cagagaggtt
    23221 ccgtggctct ggcgttccga ctggtaggaa ccgtggtgcc ttctccggaa gagctgggtg
    23281 taccggaatc cgtggtggaa ctatatcagc gcaaacgtgg tctggtactg gtcaccggac
    23341 ctaccggaag cggtaaatcc acgacgcttg ccgccatcat cgacaagatc aacaacaaca
    23401 gagatgccca tgtcatcact ctggaggacc ctatcgagta tctgcatcag cataagatgt
    23461 ccatggtcaa ccagcgtgag atcggcatag attccaataa ttatgccaat gctttaagag
    23521 cggcactccg tgaagatccc gatgtcattc tggtaggaga gatgcgtgac ttcgagacca
    23581 tcagtgtagc tattacggca gcggagaccg gacatctggt attgtccacc ctgcatacga
    23641 tcggtgcagc cagcaccgta gaccgtgtga tcgatgtctt cccaccacat cagcagcaac
    23701 agatccgtgt ccagctgtcc aacacactgg aagcagtcat atcccagcag ctgatcccga
    23761 cagcggacgg caagcaccgt gtggcagctt ttgaggtcat gcatgccaat catgcggtcc
    23821 gcaacctgat ccgtgagggc aaatcccacc agctggccag cgtcatgcag accaaccgga
    23881 aactgggtat gatcaccatg gatgaggcta ttgttcagtt gtattttgaa ggcaagatcg
    23941 acagggaaca tgcgatccag ttcgctcagg atcccgacag catggaaaat aaaatttaaa
    24001 ggaatgatcc ggaaccggct atataggagc agaccagacg acgcagcgga aagcgtacgg
    24061 ttttgtgaat ctgtagtgag gttccggagg atttttcacc aaaagacatt ttcatttaaa
    24121 atttctgata ttaattctaa gaagattccg gtagtttcac tgcccttttg cgggctctta
    24181 tgattaccca ttgtatgaat tgttttgtta agtctgatta tttttgtcag attcattcgt
    24241 tttatctgtt tctgttttgt ttcactgtta tttccataat aaataaaaca ttttaaagga
    24301 ggtattttat gtttactaca gtttgttcgg ctgcagtcta tggtctgcag gcatatctgg
    24361 tccgcgtgga agtggatctg gcagggggac taccatcgtt tcagctggtg gggtcgctgg
    24421 ggagtgaggt gaaggaatcc cgggagaggg tgagtgtggc catgaaaaat tccggtttcc
    24481 agatcccacc ggcacatatc acagtaaatc tggctccggc aggtcgacgt aaagatggta
    24541 ccgcctttga tctgcctata gctgtgggac ttttgcggga tatggaactg gtaccggagg
    24601 aagcgctgca ggatacattg tttttgggag aactgggcct gaatggggag atcaagaagg
    24661 tgaagggtgt acttcccatt gttcaggagg cggcaaaaag cggtatccga agggtggtgg
    24721 tgcccggaga aaatgccatg gaggctgcgg tgataccggg gatcacggtc tggggtgtga
    24781 actgtctgaa cgatctgctg gcggcactga atcatccgga gctggaaact gaaaaagtgt
    24841 atcagccgag gatccgggcg gaggacttgc taagggaagg aaccggacag gatacgggag
    24901 atttccggga agtggcggga caggagaatg ctaaacgggg tgccgagatt gctgcggcgg
    24961 gattccacaa tctgttgatg atagggccgc ccggggtggg aaaaaccatg gtcgccgggc
    25021 gaatccccgg aatcctgccg cctctgacac tgaaagaaag tctggaggtg acaggaatct
    25081 tcagtgtggc gggacttctg ccgccgggac aggcactgat cacaaggagg cctttttatg
    25141 caccacacca tactgtgaca caggcagcac tgatcggcgg gggaagcggc atcccaagac
    25201 ctgggatggt atcgctggcc catcggggtg tactgtttct ggacgaactt ccggaattcc
    25261 ggaaaaacgt gctggatggc atgcgacagc ctatggagga gcgtaaagtg cagatcgcca
    25321 gaagtgccgg gatggtcact tatccgtcgg actttattct ggtggcggct atgaatccct
    25381 gtccatgcgg gtattatccg gaccggaata aatgccgctg tacccagccc cagatcgaga
    25441 aatatctggg aaagatatcg ggaccgattt tagaccggat tgatctgtgt gtggaactgc
    25501 atcccgtgga tattctgcat ctgcagaaaa cgaaaacgga aaaaagcagt gaagatatga
    25561 agcggcagat ccggcatgca agagaacggc aggcggagcg ttttgcagga acggattgcc
    25621 gtttcaatgg ggacatccgg tctgcacagg tggaagaata ttgcccgttg ggagaggagg
    25681 gtcagaaagt catggaacag ctgtatcgta cgctgcagct cagtgccaga gtttatcacc
    25741 ggattctcaa aactgcccga accatagctg atttggagga ccgggaagag atcacggggg
    25801 aacatttgct ggaggcggcg tgttatcgac cttcggagac gtattggaat taattgtgtg
    25861 ccggaaccgg gtacattaag tgtccggcaa gaaaaagggg ccggtacgaa aatcacggaa
    25921 aacgtgcggg ctgtgtgaaa acagggaaag ggacaggtat gtttatggcg gaggaagaga
    25981 gaaaatatgc atattttttg tgcagcacct atacaatcgg gggcgtgcgg gcagaacggc
    26041 tgcttgcacg cttcgggtca ccccgggcgg tatatgaggc ggaggcagag gaatggaggg
    26101 agtgcgtggg caattctgcg gcggaagcct tggaacggca gaagaaaagc ggagagtggg
    26161 aacgggaata tggacagctt tcggaacaac agatccactt tcttttgcgg gaggagaaag
    26221 gatttcctgg gaaactggca gaaattccgg atccgcctta cggcattttt taccggggaa
    26281 aactgccgga tgagaacgaa ccggcggtgg cggtcatcgg tgccagagag tgttcggaat
    26341 acggcagata tgtggccgag gaactgggac agtatctggg aagagcggga atacaggtca
    26401 tcagcggaat ggcccggggg attgacggga tcagccagca ggcagcgtta tctgcgggtg
    26461 gaacttccta tggtgtgctg ggatgcggag tggatatctg ttatcccgca cagaatcgaa
    26521 gattgtatga agaactgttg gaaaaagggg gattgctttc cacctatgcg ccgggaacaa
    26581 agccgctgcc ccagtatttt ccaccaagaa accgtattgt cagcggactg tcggatgctc
    26641 tgatcgtcat tgaggccaga cagaagagcg ggacgttgat cacggtggat atggcactgg
    26701 aacagggcag ggacgtctat gcggtgccgg gcaggcttac ggacaggctc agcgacggct
    26761 gcaatcatct gctgacgcag ggagcgggag tgtttttatc tccggagagt tttgcagagg
    26821 attttttgcg gagctgggag gaaaaacagg gactttccgc agatggctcc ggaggaaaga
    26881 acaggcggaa aaataatagg gaaaccggca gaaatacggc aaaagtaaaa ttgcccaaag
    26941 aatccctgca aaagatcttg gaatgtctgg aacctgtgcc aaagacatcg gagacgctgt
    27001 tgcaggaact ttgggagaag ggagaatata cggacatttc cgcactgaac cgggcactga
    27061 tggaactgga acttttggga tatgccagac agaacagtcc gggacatttc agcacttctg
    27121 cgaattgaca aggctttcgg aaaaggggta tagtaataga catatgagaa tgacgagaaa
    27181 ggtcaggtgc ccttatgaac gataaagata cttacagaat gcataccaaa gtaatacaga
    27241 tcggagaccg gaagatcggc ggcggcaatt ctatcgccat ccagtccatg accaatacga
    27301 agaccgagga tgtgaaggca accgtagagc agatcctgcg tctggagagg gcaggctgtg
    27361 agatcatccg ctgcacagta ccgacgctgg aggcggcagc agctatccgg gagatcaaga
    27421 agcagatcca cattccttta gtggcggata tccattttga ctaccgtatg gcactggcag
    27481 ccattgagaa cggtgccgac aagatccgca tcaatcctgg gaatatcgga aatccggaga
    27541 gggtaaaggc tgtggtagat gcagcaaaag agaggaatat cccgatccgt gtgggagtga
    27601 acagcggttc cctggagaag ccgctggtgg aaaaatacgg tggagtgact gcggaaggta
    27661 ttgtggagag cgcactggac aaggtacaca tgatcgaaga tctgggatat gacaatctgg
    27721 tcatcagtat caaatcttcg gatgtgctga tgtgcgtgaa agcccatgaa ctgctggcag
    27781 gccgtacggt gtaccctctg catgtgggca ttaccgaatc cggtactgtg aacagcggta
    27841 atatcaagtc agccatcggt ctgtccctga tcttaagcca gggcatcggt gacaccatcc
    27901 gggtatccct gacaggagat cccgtagagg agatcaaatc cgcaaagctg atcctgcgta
    27961 cattaggatt gcgcaagggc ggcattgagg tcgtatcctg tcccacctgt ggaagaaccc
    28021 agatcaatct gatcgatctg gccactaaag tggagaaaat ggtagaggat tacccgctgg
    28081 atatcaaagt ggctgtcatg ggctgcgtgg taaacggccc cggagaagca aaagaagcgg
    28141 atctgggagt tgcgggcggt atcggagaag gtctgctgat caagcatggc gagatcgtaa
    28201 aaaagcttcc ggaggatcag ctgctccccg ccttgaaaga ggaattggat cattggagtt
    28261 aaaataaatg agtaaaccgt tttttgaggt atttccttcg ttacagttga atacggacat
    28321 tcgtgacctg atggggcaga cagaagtgga gagagtctcg gcgacaaaac gcagagactt
    28381 tcttcgggtg tacttaaaaa gtacccgcct gatccagaaa gcggacatct ggaccacgga
    28441 acaggagatc aagaagcagc tgttccctca ggccaacctg acagtgaaaa tatacgaaaa
    28501 gttcgaattg tcttcccagt acaatccgga aaagctgatg gatatttata aggaaagtat
    28561 tctggaggaa ttccgggagt acagccatat ccagtataat gccctgaaga cggcgaaaat
    28621 cgagtatcct tcggaaaatg aaatgctttt aacgctggaa gatacggtat taaatcacag
    28681 tatggaaggg gagatcctgc agattctgga gaagatcctg gtggaacgtt gtggattctc
    28741 cgtgaagatc catacggctt atgtggaaaa agagaccggg cgtttccagg aggaagagga
    28801 agcaaagatc cgtctgaagg tggatgccat ttacagtcgt acgaaaggca gaagagaaga
    28861 aggagagcag actgcatcgg cggaaggaag cgggcaaagt acagcacagg atccggaaac
    28921 cggcaaggct gcagagaccg cacaaaagcc gaagaccgag aatgccggtg gcgtgaccaa
    28981 gagctttcag ggtggcagcc gcggtgaatt caagcgtgga gaattcaaaa agggcgagtt
    29041 tcgtaagggt ggtaactttg aaaaaggtgc gttgaagaga tcagacaatc cggatgtcat
    29101 ctacgggcgt gatttcgaag aagaggccat gaagattgag gaactcatcg gagagatggg
    29161 tgaggtggtc atccgcggta aggtactgtc cgtggatacc cgtgacatca agaacgagaa
    29221 gacgattatt attttcaata tctctgattt taccgatacc atgaccatca agatgttcgt
    29281 gcgtaccgag caggtgaagg aagtgaccgg ggacatcaag ccgggagctt tcttaaaggt
    29341 caagggaatc tgtatgatgg ataagttcga ccacgagctg gccatcggtt ccatcgcggg
    29401 aatcaagaag atccctgatt ttaccaatac gagagtggat acctccgcca gaaagcgtgt
    29461 ggagctgcac tgtcatacaa agatgagtga tatggacggt gtctccgagg ccaaggacat
    29521 tgtaaagcgc gcctacaaat ggggacatcg ggccattgcc atcacggatc acggcgtggt
    29581 acagagtttt accgatgcaa accatgtctg ggatgacctg tggaaagcgg aaaaaggcaa
    29641 gcggaaagaa gccggagacg agaatccgga caagcaggat ttcttcaaga tcatttacgg
    29701 tgtggaagcc tatctggtgg atgatctgaa agagatagtg acgaatgata agggacagag
    29761 tctgcatgag gattatgtgg tattcgatat cgagaccacg ggattttccc cggtcaacaa
    29821 ccgcattatc gagataggtg cggtaaaggt atccggtgga gagatcgtgg atcgtttttc
    29881 cacctttgtc aatccggatg tgcccattcc cttcgagatc gaaaagctga ccagtatccg
    29941 ggatgaggat gtcatggatt cccctcagat cgacgtgatc ctgccgcagt ttctgcagtt
    30001 ctgtgaaggc tgtatcatgg tggctcacaa tgccagcttt gatatgagtt tcattatgga
    30061 gaattgcaga agactgggct atccgcagga gtttacctat gtggataccg tgggcatttc
    30121 cagagtactt ttgaagaacc agtcgaagca tacgctggat gcggtggcca agaccctggg
    30181 gatttccctg gagaatcatc acagagccgt ggatgatgcg gagtgcacgg cccatatttt
    30241 cgtgaaattt attaaaatgc tggaagagca ggacattcat aatctgacgg aagtcaatgc
    30301 actgggagct tccagtgtgg atgccgtgaa gaagatgccc tcttaccatg ccattattct
    30361 ggcgaaaaat gatctggggc gtatcaacct gtatcgtctg gtgtcccagt cgcatctgac
    30421 ctattttagc aaacatcccc ggattcccaa gagtctggtg atgaagtaca gagaaggact
    30481 gatactggga agtgcctgcg aggcgggaga actgtaccgg gcactgttgg acggacagtc
    30541 ggatgcccag attgccagac tggtgaaatt ctatgactat ctggagatcc agccctgcgg
    30601 caacaacaaa ttcatgatcg cttcggaacg tgtgaggaat gtaaattcca ttgaggatat
    30661 tcagaatatc aaccggaaga tcgtgtccct gggagaacag ttccacaagc cggtagtggc
    30721 tacctgtgat gtgcatttcc tggatccaga ggatgaggtc taccgccgta tcatcatggc
    30781 gggacgagga ttcgatgatg cggatgatca ggcaccattg tatttacata ccacggaaga
    30841 gatgctggaa gaattctctt atctgggaag tgataaggcg gaagaaatcg ttattaccaa
    30901 taccaatatg attgcagaca tgatcgagac tattgcaccg gtgcgcccgg acaaatgtcc
    30961 tccggtcatt cccgacagtg ataagacact gacggagatc tgttataaca gagcccatga
    31021 aatttacgga ccagatctgc ctcagatcgt agaggcgaga ctggaaaagg aactgaattc
    31081 cattatcaag aacggattcg ccgtaatgta tatcattgcc cagaagctgg tatggaagtc
    31141 ggtagaggac ggttatctgg taggttcccg aggatctgta ggaagttcct tcgtggcgac
    31201 catggcggga atcacggagg tcaacccctt aagccctcat tattattgca gtaagtgtca
    31261 ttatgtggat tttgacagtg atgaagtcaa ggcctatgcc ggtaaggcgg gaatcgatat
    31321 gccggacaag ctgtgtccgg tgtgcggaga aaagcttcac aaggacggat ttgatattcc
    31381 tttcgaga
//
LOCUS       DAWEJW010000019        30937 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_626_length_30937_cov_66.476491, whole genome shotgun sequence.
ACCESSION   DAWEJW010000019 DAWEJW010000000
VERSION     DAWEJW010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 30937)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 30937)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..30937
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_626_length_30937_cov_66.476491"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    25265..25364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    27904..28003
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7258 a         8397 c         6168 g         8914 t
ORIGIN      
        1 atcagcaaaa tccgctttcc ctttttcgat cccagcagat acaccacata tcccagcagc
       61 aggtatccgt gaaatcccgc attatacaac agggagatca gcgggaattg tgttacaaaa
      121 tgtatcattc cgatcagaaa ttctctcatg cctttcagct gtggaggaaa tcctatttcc
      181 atatagaact cttccatcga aagctgctct ctgccaaccg tagtctctat gatcggcgtt
      241 cccagtctgt ccttccggtc cgtataaaaa tatccgtaag tctggttaaa aaatgcctgg
      301 aaataagtat ccggatgctt gcagaactgt gcaaaccaga ctttaaaata attcttcagt
      361 tgttccttcg tgggatggga tatcatctgg tttttggccg catcggatat ctccggtgaa
      421 taatgtgccg ttcccatctg atcatagtca tcaaacaatt ccgacagaat cgcccgttct
      481 tcctccgtga cctctgctcc gtgatccctc tggtatcttg ccgtctgttg aataggggcc
      541 gatagtcctt ccgccagtct cccctcttca atatcatgtg ccggcatata tatctgctct
      601 atatagaaca ggatcataaa accggttact gctgtaaaca agtataattt ccactgcttt
      661 ttatgataga aaaacagaca gatcagcgca cccaccaaaa ggtatctgcc ttcttttctg
      721 cagagtgtca taccgcaaga agcacagaga aatacgatct gtttccacca ttttttcttt
      781 tccgttccat ctaccaggat atccgcaaat gacactccaa aatacaacag gctgatatag
      841 tactgtgcat ccttcaccat agtaaatccc cacataggaa atagcggaaa cacagtaaga
      901 aacagaagtc cgaaccaacg gatccagacc ggagctttca tttttttcat aacgcagact
      961 agatatgaga ttccaactgc ctgcaatatg gactgtatca aagtataggt aaaaattcca
     1021 agattgtcgg atcccagtac gactcttccg aaagccagac atcttcccat gattgccgtg
     1081 gataccaccg ggtgtttatt gctgttttca ataattccca tgtacctcca cacctgttca
     1141 aatgcatccg cctgaatcgg tcccgggaaa aaacttatca tatagggcag cgcacacagc
     1201 attgctatgg caaaagtact ccagaaggga tgggcttcga aaataaaatt ctctattttc
     1261 ccctttattt ctctcctgcg cactccttcc caatgttccg acacataggc tcccaacgca
     1321 atgagactct catacaggtt gtaataaccg gccatcacca ccagcgccaa caggaactga
     1381 gcaaagtttc caaacacata gttccagctg ccctgctcat gatagctcgt ccccattacc
     1441 gtagtaaccg caaaaaaggc gcacaaaaca tgtagccatc tgttttttct cagagattcg
     1501 tccttcgtga tccagcatac tgctcccaca cccatggtca gaaaaaccat ttccacatgc
     1561 agaccgtcca atccgtcact caatgttgtc agacagcccg gtgtcaacgc aaatatcatg
     1621 aataagatcc ggaaaaacca tccaacgttt ttccatttac tgttttcctt aagcaactgt
     1681 ttcatctgct gtacccttct tatttctcca atatttgaac gctttattca tagtttccaa
     1741 aagaatgaga taaatcaaag catttcccag ccagtaacca cctattatga tcactttatc
     1801 cggatgtatc ttataataat atctggttgt agcctgtgca tcttccgcag caaacaaccc
     1861 gccgttcctt tcaagacgta tattctccgg ggccacaccg tttccatgtt ccgcatacat
     1921 aaaccggata ttgcattctg cggcttccgg tattgtaagc gtccaatgat atgtctgttt
     1981 tccggaaagt gacaccccca ggggaagtgc gtagaaatga tccgcactca tacttccgag
     2041 aggaaaatcc tgtgacatca gctctgtatt cttttcatca tatagtacaa aatgcaatac
     2101 ctgatctgtc accgcttcct gctcaaatcc cagcgcaaat tctatctgat ccaatctgga
     2161 atacagcggc ttaaaatact gtgtgatctc cgtccgttcc gtgatgggct cactatacac
     2221 actgtattca ctgtaagagc tgctcatata gttattatac accaggtatc ccggccagat
     2281 ggctaattcc agtgcaatga gcagcaccgt cagcagacct tttcctatga cctctgcctt
     2341 gttgattttc tttttctttt gaccccgaca tcataatata taaggataca cgagatgaag
     2401 aatttatgca aacaaaactg gtatcagatc acattacttt ttctgttttg tgtcacagta
     2461 tttttaagat tctataaact gaccgtttta ccggatgttt tacatataga cgaagccgct
     2521 cttggatata actcctggtg tctggccaat tacggtaccg atcgttatct caatgtgctg
     2581 cctttctatc cgcagaattt ttacggtggc caaagccctc tctatactta ccttgtcgta
     2641 ttgctgatca agtttttcgg gaaggggaat ctgtccgttt ttctggtaag atttcctgct
     2701 gcctttgcca gtcttcttct gtggatcacc ggcaccagga gcatgtccct tatctttgat
     2761 aataaaaaaa tcaccctcgc tgctgctctt tttctgtgtt tctgcccgta ttttgtgatg
     2821 tccggacgtt atgccctgga ctgcaatctg atgttgtgct gcatttcctg ttctgttctt
     2881 gtactgcttt tgtatctgaa aacagggaaa ctgcatactt tacttctgtc cggagtctgc
     2941 ttcggtatca gcttgtactc ttatgcattg agttatctga tcatacctat ttttctggtt
     3001 ctgatctctc tttacatgct ttaccataaa aaaatcacgt ttcccagaat actgttgtgg
     3061 gcggtcagtg tctgcattac ggcattgcct gtgatccttt ttgcctacac acttctcttc
     3121 catgcagagc caattcattt tctcggcttt acaatttctc ctatttccgg caaccgcatg
     3181 gatgaaattt ctttttcctc attgggtgca aatctgtgga acaatcttaa gatcactctg
     3241 acatacagta tttatcctat ggattccgtg gataaattct ataccttata cccggtttcc
     3301 atccctttta tcctgttggg aatgttatgt tccgtttatg acttcggaaa ttccttccgg
     3361 acacgctgtt ttcattccgg cacagtatat ctgttctatt tcatcagctg ctcctttgtg
     3421 gttgctctga cacctacaga tcatctatat cgtgccaaca gcatctatat ctgttatctg
     3481 ttcttttttc tccgtggtat ccgggcatgt tgcgattttc ttaccgttta ccggaaagca
     3541 tttctgagca ttctggctta tggctatgtg ctgtggattg cttcgtttat gcgttactat
     3601 tacactatat attccgtgct ggatctgcat acctatgcaa attccttcta ttttgccgat
     3661 atttccgaca ttgtcaccta tattgatgaa aatctcgggg ataaagagat ctatgcggac
     3721 tgcgttgatg tagaggagtt tttgtatttt tattatccta tttctcctta tgagcgcgtt
     3781 cccctgtccg cccgggaaga agacgatgaa atccccaagc attatatact gaatacttcc
     3841 actccgctct ctccttccgc agcctacatt gtccgcaaag aaaatcagga atttatttct
     3901 tatctgaacg attccggact ttcctgccat acggtagaat tttcaccgta ttacttattt
     3961 tattttgatt gattgcgagg tatccacaaa tgaaggatct gaaatctctg gtgaaaaaat
     4021 actggtattc cgcagccatc ctgctgttgt ttctcattgc ggtcttcctg cgcgtttatc
     4081 gtctggaaag cctgcctgac gttcttcata tcgatgaagc aggactgggt tataatgcct
     4141 ggtgtctggc tcattacgga accgaccggt atctcaatgt acgccctttt taccctcaga
     4201 atttttatgg tggtcagagt cctctttata cctacttact ggctctgctg atccggacag
     4261 tcggacaggg caacctgtct cttacgcttc tcaaaattcc cgccgtcctt gcaagcctgt
     4321 tgctgttttt tgtcgggacc aagagtatcc gtctggtgtt cgatgatcaa aaatggagta
     4381 ttgctgctgc tttcctgttg acaatctgcc cctattatat catgtccgcc cgttttgccc
     4441 tggactgtaa tcttatgttg tgctgctcag ctgtagcatt gctgtttctc atccgtttca
     4501 cacagactaa gacccttcgg aaccttatat tatccggagt cttttttggt atcacgatgt
     4561 attcgtatgc actgagttac ttcctgatcc ctatttttct gatctgcatt tccctgtatc
     4621 tgctatacac gaaagaaata tccttccgcc gggttctgct ctgggctgca tgtgtatgta
     4681 ttacagctct gcccgtaata ttatttgtat gctccctgtt attccactgg gaaaccattc
     4741 attttctggg gcttaccata tctcccatcg cctccgagcg catgtccgat gtgggagtca
     4801 cttctttctg ggaaaatata aaagatatca taaagattac cttgacatgt ggttcctacc
     4861 gtttagatgc cgtacctaaa ttttacacgc tttatccctt atccattcct tttattgttc
     4921 tgggatttat cggtgcagtt tacagttttc ttgtctctct gtacaagcgg acttttcagg
     4981 ccgacagtat ctatctgctg ttctttctca gcggtctcat caccattggt ctgtccggat
     5041 ccggctatgt ctaccgggct aacagtttct ttatctgtta tctcttcttc ctgataaaag
     5101 gacttttcct ttgctgccgt ttcctgtcct cttatcacac aggctttatg ctccttttgt
     5161 ccgccggtta tctcttatgg tgcatctctt tctgcagctt ttatttccgg gtctatacca
     5221 tgaccgacgt ctgcccgaat tatctgtatt ccatctcctt ccgggagtgt gtggatgatg
     5281 tgaatctcaa cctggattat gcggatctct atgtggatct ggtcaacatg aaggagtttt
     5341 tcttctttta ttatcccgca tctccttatg atactcagga tgaagaaacc tttaccgacg
     5401 gtatacacac ttatcatttt ctcaccgact attccacgga gatcagtcct tcttccgtct
     5461 atgtggtata caaaaagaac aatgaattcc tgcagaatct gtcagagtcc ggactggctt
     5521 accagacctg ggaatacccg tattattatg ttatttattt tgagtaaccg tgctttcctc
     5581 tttcttcgta ctcagtgtca ctccatcacc taccggcagg atcaccgtct ccagttccgg
     5641 atgatgtttc aattcataca gatattcccg catccttgca tgaatggtcc ggttccttct
     5701 ggttacggca aaacgggatt ctatgatatc accgtcctgt aatacgttgt cggagatcaa
     5761 cagtccgcca gggggcaaca ggcgtaagat atccggcagg aaatgaatgt actgtccctt
     5821 cgccgcatcc ataaagatca catcataggt gccttccagt tcccgcagga tctccgtagc
     5881 atcaccctcc agcagagtga tccggtcttc cctgcctgcc ttacggaagt tctcctttgc
     5941 aataggaatt cttttctcat atttttcgat cgtcgtgata tgacaatcct ccggtgcata
     6001 ttcactcatc agtaacgcag aaaagccgat agcagtcccg acttccagga tgctcttcgg
     6061 ctttgtaaat ttcagcagaa atttcagcag actttgcatg gatctgcgta tgatgggaac
     6121 ctgtgtctcc agagcgaatt tttcaatctc attcagccac gccggattcc cgctgtccag
     6181 ggaatcgata aacgcggaca ttctctcgtc tatgatcata ctgtccctca tttcacactt
     6241 tactgtgcaa ctgcagtggt atcctgtgtc tcaccggtaa tcgcactgcc gttgtctata
     6301 gtctccactg tctcttccgt tgcgctttcc gtctgtccgg ccatgacttc catcatctct
     6361 tctgcggtca tggctgtact caattcgtag gttccgggct tccagtcctt tttatactcg
     6421 gatagcagat actgtaatgc aaacagtctg ccatctctta ccagaccttt ttcctgcaac
     6481 gtattaccaa tctctgtcgc agacatgtct gaagtcaggg tcacggtgat cttgcggcct
     6541 tctccgctgg agatcgcagg ctccgtaaaa atacgataac catagtcgta gcatgtagac
     6601 gctccggtgt acaccagata taccacgatc actgcaaatg ccacttttaa gatagcgccc
     6661 aatacggcac ctgccaaatt tgctgccttc atgtgttctg tcctttctta tccaagtaat
     6721 ccgcatttcg atccatacat tcgcaaaatc gtctcttcga gactttcctt ttgctcactc
     6781 aaagtgcatc tgatcggtaa cgtaaagtcc gatcccctgc atcatacggc aaaatgccag
     6841 ctccgccgcc agaaaatccg acaccagtgg attctcccgg aaagcttcgt tttctctggt
     6901 aaactgatcc agcttatcaa agttggtgtc cttgctggtc tgcaattcaa aatttctggt
     6961 ccggaattca tcgatctgtc tcttaagttc cgattgttcc ttcacgcggg ccagctgctt
     7021 ctgatattcc agataagcgt cggtattacg aatcgcctgt accatctgct ctacagcctg
     7081 atttacattt tcgtccgatg taatcttcat ctgcttacga cctttccagc aatcgctgct
     7141 attacacttc cataatgatc ggaaggatca tgggacgtct cttggtcttc ttccatacat
     7201 aatcgccgag gacatccttg gtggtggtct tcagtttacc ccagtccgta attcccttat
     7261 ccagacatgc ctgcagcgca tcatccacgg tgcgtctggc ttcctcgata agttcatcgg
     7321 attccttcac atatacaaaa cctctggata caatgtcggg acctgctacc agttctccac
     7381 tggccttgtc cagactcatg accacgatca taataccatc ttctgccaga tgctgtctat
     7441 ctctcaatac tacatttccg acatcaccga cacccagacc gtcgaccaga atagctccta
     7501 cgggtactct gccgttgacc ttggcctgtt cctcgctgac ttccaataca tctccggagg
     7561 acaggatgaa gatattgtcc ttactgatgc ccagactctc tgcaatcttg gcctgtgcct
     7621 tcagatgctt gtattcacca tgcacgggta ctgcgtactt cggatggatc agggtataga
     7681 tcagcttgat ctcttcctgg caggcatgtc cggatacatg agcatcctgg aaaataacat
     7741 ctgcgccttt gcggagcagc tcgttgatga tcttggtgac ggacttttca ttgcccggga
     7801 tagggttgga ggagaagatt acggtatctc ccgcaccgat cgtgatctta cggtgcatat
     7861 tgttggccat acgggacagg gctgccatac tttcgccctg gcttccggtg gtgatgatca
     7921 cctgctgctc atcgggataa ttcttcatct gatccagatc caccagtgta ttctcgggaa
     7981 tattgatgca ctccagcttg atggcggtat ccaggatatt taccatactg cggccttcca
     8041 cagccacttt acggccaaat ttgtatgccg tattgatgat ctgctgtaca cgatccacgt
     8101 tggatgcgaa agttgccacg aaaattctgg tgttcttatg ttcttcaaat aaagtatcaa
     8161 aggtcttacc cactgttttc tcagaagggg taaagccggg acgctctgca ttggtggagt
     8221 cgcacatcag tgccagtaca ccctttttgc cgatttctgc aaaacgctgc aaatcaatag
     8281 catctccata taccggtgtg tagtctacct taaagtctcc ggtgtgcacc acaataccgg
     8341 caggggaata aatggccagt gctgcggcat ctacgatact gtgattcgtc ttgatgaatt
     8401 ccacacggaa atcgcccaga ttgatggact gaccgaactt cactacttta cgcttggtct
     8461 tcttcatgag attgtgctcg gtcagcttgt tctcgataat acccatggtc aggcgggtag
     8521 cgtagatggg aacattaata tcccgcagca cataaggcag tgctccgata tgatcctcat
     8581 gtccgtgggt gatgacaaaa ccctttacct tgtcgatatt gtccttcaga taagtcacat
     8641 ccgggatgac cagatcgatt cccagcatat catctgcagg gaaagacaga ccacagtcca
     8701 ccacaataat actgtcctca tactggaagg cagtaatgtt cataccgatc tgctccaggc
     8761 ctcccaaagg aatgatcttc agacctgaaa cgttactatt ctcttttttc tttttcaacg
     8821 aaattacctc caaattctat acgtctatac atcttgcttc gtgtatgtat tccggcgtta
     8881 gaatgagata gtatgctctg caaacaaaga tctgtgatct cacgcctgct ccttccggct
     8941 ctcctctcaa agactcccgt ggagatcaga catcccagat cttacgattc catcaaaata
     9001 acggcatctc tcatgccctt cgtatattcc ctgtcacttt tggcacaaaa ccattatatg
     9061 ctgaaaccgg caaagcctcc aatatccctc tctgccaatc atacaaatca tatataaata
     9121 taagaccgtc cttctcgcag aactgcctgt atttatcata tatcaaggtc ataagcctga
     9181 aaacacaacg tgcttgcacg ggagtgattt tatggcttaa tgacctgccc caatatgagc
     9241 ataaaagcgg agcgaatgct tcgcaatatg gtgatattgg aacggattgt gtgagtgcat
     9301 ggcacggaac aatccatagg atatgatcta aattaaatca acgtcctcta acatatccgc
     9361 aaatactccg gataccgctt ccagttcttc atcatcggag actacatcat aaatgctctc
     9421 ttcttcacca tcctgagaca tatccttcag gatcagggca tctccatcgc cctcttccac
     9481 atcagtcacc aggatgtaat tgtgtcctcc gatcctggtc tgttccagta cataaaattc
     9541 tacaggttct tcgccttcgg ggcgaaatgt gatcttttcc aatttactca ttctgtttcc
     9601 tttttcttct cattgttcct gcgatccaga tagccctgca ggatcagagt agctgcgatc
     9661 atatccacgt aatccttacg gttctcacgg cggattcccg cttccatcat ggctttgtcc
     9721 gctgccacag tcgtcagtct ctcatcccag aaatataccg ggagaccggt cctgcgctcc
     9781 agcttgtcct tgaactcttt ggtcagttcc acgcggacac cttcggtatc gttcatgttc
     9841 ttgggaagtc ccagtacgat ctcttccact tcattctcga cgatcagttc ctcgatccgg
     9901 gccagcgtct gtcgtaattt attctcttct ttgcggcgaa tgatctccac accctgcgct
     9961 gttaagagca gactgtcact gatcgccacg cccacggtac gtgagccgaa atccaaaccc
    10021 atgatacgca tgtgggtcct cctgataact gtaattattt ccagtgtttt gtcctgatat
    10081 actccgtgag cagttcttcc accagttcgt cacgctctac cttcatgata aggctgcggg
    10141 catttttgtg actggtaata taagtagggt ccccgctcat gatgtaacct acgatctggt
    10201 tcaccgggtt gtatcccttt tccgtcagtg cctcatatac ggtagagagc accagcttca
    10261 cgccggtctc aggttctgtc tctaccttga agtattgtgt atttcctaag tcctgcatac
    10321 gatcctcttt ttctgccata gggcaaatct ttccgttatg ccttatatta ccgcatttac
    10381 tttgaaaatt caaggggtga tagtaatagg atttcatccg tcaaaaaccc ctgtaccctg
    10441 ccgcatatca cggtatttgc aacatttttt ttattttcct tcacagagac agccagtttg
    10501 acataatctc tcgtgtgtcc cagcaggtat tccgttccgc cgatctcctt ggtctcctcc
    10561 agaagcacct ccgcctcccg tcccacatag tgggtccgga actgcttcga ctgttccttc
    10621 tccatagcca gaagttccgc acttctggtg gtcttcacgg gatccggcac ctgatgagac
    10681 atcacggccg ctctggtgcc tgcccgtttg gaatacttga aaatatgcat ctcgaagaat
    10741 tgaatccggt ctaaaaatcc taccgtctgc gcaaactctt cctcagtctc ccccgggaaa
    10801 cccacaatga catctgtggt gatggcggga tggtcaaata ccttacgaag cagcgcaaca
    10861 ctctccgcat attcttcgga agtatattta cggttcatac ggcgtagcgt ggcatcacag
    10921 ccgctctgca aggacagatg gaaatgggga cacagcttcg gcagtgccgc cattctggct
    10981 gccgtctcct ccgtaataat acggggttcc agggaaccga tacggatccg ctcaatccct
    11041 tcaatagtat ggatccgttc cagcagatcg atcagcttac tgctgccgga ataatctctt
    11101 ctttcctcgt cttcttttaa tacagaaacg ctttctcccc gcttccaggc ctcctcgtcg
    11161 aaatcaatgc cataagaact gatatggata cctgtcagta cgatctcccg gtatccgttc
    11221 tgtgccatgc cggtgatctc ccggatgata tcctccgccc tgcggctgcg gaccctgcct
    11281 ctggcatagg ggataatgca gtaagagcag aactgattgc atccgtcctg aattttaata
    11341 taggcccggg tgtgctctgc cgtctgtttc agagtcattt cttcgtattc tgcagtatgg
    11401 ttaatgtcta taacagtagt gtcatgcaga gtcttgtcca caccatgtcc ggcattttct
    11461 gcattctctg ccaccgcatc ttcctgttcc agatcctgca aatattcctc cagaatggat
    11521 gccaggttct tcttgcggtt gttgccgatc gccagatcga tgcagggatc ctgctcaaca
    11581 tcttcccttc cggtctgcac gtagcatccc acagccacca ccagtgccgc cggatttttc
    11641 tgcttcgcac gatgaagcat ctgtctgctt ttccggtctg caatattggt aactgtgcag
    11701 gtatttacaa tataaatatc tgcggtttca tcaaatggta caatagtata acctttttct
    11761 tgtaacattt gttgcattac ttctatttca taggaattaa ccttacatcc gaggttgtgt
    11821 aatgctacac ttttcatagg attcctcact ttttttgtat tgttttcgtc ttgacttttg
    11881 agcgttcccc cattaatata aagtcagaag gtatgacaca cattaaggag gtagtcaaca
    11941 tgaagacagt tcagatttct ttaaattcta ttgataaggt aaaatctttc gtaaacgata
    12001 ttactaagtt cgattatgat ttcgatcttg tatccggcag atacgtaatt gatgcaaagt
    12061 ccatcatggg tatcttcagc ctggatcttt ccaagcctat cgacctgaac atccatgcag
    12121 aggatggcgc tgatgaggtt ctggaagtat taaagcctta tctggtataa gttaccgatt
    12181 cggaattccg aatacatagt aaaacagaga acaccttgag agacgcaatc ctcttgaggt
    12241 gttttttata tgccattcca aaaaagtaac tattcagtac ctctgtttcc ccggttctga
    12301 atagttactc tgcattcgca cttatgcttc cgcaatgatc agttctttgg tctccagtgc
    12361 ggtcttcacc agttcatcga tcttctcatc cagattgtgt tcatgtctgc ggatcacctt
    12421 cacgttgggt ttcgtcacag gatgctttgc cacgaaaatg gtacagcagt cttcataagg
    12481 aagaatggaa gtctcatagg ttccgatctt ctcggatact tccacgatct cctgcttgtc
    12541 aaaaccgatc agcggacgat agaccggcag ctcgcagact tcattggtgg cgatcaggga
    12601 attcatggtc tgagatgcca cctggccaat gctctcaccg gtgatcaggc ccaggcactc
    12661 agtctctttc gcgatctgtt ccgcaatccg catcatataa cggcgcatga taatggtcag
    12721 ttcctcatga ggacatttct cataaatata taactgaata tcggtaaaat tgatcacatg
    12781 cagatagatc ggtccggtgt aacgggcaac gatcttcgcc agatccacta ctttctgctt
    12841 cgcacgctca ctggtgtaag gcggtgcatg gaaatatacc gcatcgatct tgacgcctct
    12901 cttggcgatc atataacctg ccacgggaga atcaatacca ccggagagaa gcagcattcc
    12961 cttgccaccg gtacctacgg gcattcctcc gggaccgggc aggatcacgg agtagatata
    13021 gatttcttca cggatctcaa tgttcagcat aatatcggga tgatgtacat ccaccttcat
    13081 attgggataa gccttcagga tagcttctcc catatccatg ttgatggtca tggaatcctt
    13141 ggggtaattt ttccgggcac gtctcgctgc caccttgaac gtctgattgc aatcgggata
    13201 tacttttgca atgtagtcaa tcacggtctc acacagcttc tcaaagccct catcctgtac
    13261 gtgcactacc gggcagattc cggagattcc aaagactctt tgtaagtgtg ccacaacttc
    13321 atcgtagtca aattctgact ctgcatctac gaagatacgg ccctgtgtct tgtgcaccag
    13381 gaattctccc tcacactttt tcagtgcata cttaatctgg cgtaccagag catcttcgaa
    13441 caggtaacgg ttctttccct taatgccgat ctcggcatat tttatcaaaa atgatgtaaa
    13501 cacgttcgtt tcctttctga gtgtttctca aatcgcttat ctgcgactgt atctgcgtaa
    13561 cataggtact atattgtaca atgcctggag acatacgtca atctcttctt ccgtggtaaa
    13621 tacggaaaaa ctgaaacgta tggtggaatc cagcagggat ttatcgatgc cgatggcttt
    13681 cagtgtagca gaaggctgcg gcttgtgtgc agagcaggcg cttcctgcag aaacatagat
    13741 tcccttgtcc tccagtgcat gcaggagcac ttcacttctg acgccggcaa aagatacgct
    13801 gacaatgtgt gccgccgttc cctcaccgta cggtgcatcc ggcaaaagtc cgttgacctt
    13861 cacctgatcc agtctttcca caccttcaat gaatcgtctc ttgcaggcgc agagtctggc
    13921 atgatcctca tcaaaatgac tatagagcat ttccgctgct tttgcaagtc ccgcaatacc
    13981 gggcacattc tcggtaccgg agcggaggtt ctgctgctgt ccgccaccga atacgatggg
    14041 ctggatcttc acccgttccc ctacatacag cagtccgacg cccttcggtc cgtggatctt
    14101 gtgtccactg acggacagca gatcaatgtg catcttcttg ggatagatcc gggattttcc
    14161 gaatccctgt accgcatcca catggaacag cgtatccggg ttcatctgtt tgatcagagc
    14221 tccggcttcc tcaatgggct gcagggctcc gatctcgtta ttcgtgtgca tgatagatac
    14281 cagaatggtc tccggtgtca tggctctgcg taagtcttcc agccggatac gtccgcaggg
    14341 atccaccggc agataagtga cacgataccc ctgggtctcc agataacgca tggtctgcag
    14401 gatagcggga tgttcgatct gcgtcgtgat cagatgattg cctctgcggc aattcgccag
    14461 ggcgcagccg atcagtgcca tattatcgga ttccgttccc ccggaagtaa ataaaagctc
    14521 tctctcctcc accttcagaa tccttgccag agtctccttg gcatagcgga ggtatttctc
    14581 tgcctccact cccttcatat gcaggctgga gggattaccg taatcttcac acatgatctt
    14641 cgtcatcaac tctgccactt ccgggaatgt ccttgtggta gcggaattgt ccatatagac
    14701 ttccattctc ttcttctcct tgctatctat ctcttcagta actattcagc gccctattcg
    14761 ccaaatcgac tcttccgggc tttccttttg ctctgaataa ttactatatt cttcttatga
    14821 ttaaaatatg ccgcaggcag gcgatccgtg ccgtccgctg tctaattttc caactgatac
    14881 atcagccatg cgatagtcgt aaatccggcc gtctccgtcc gcaggatccg ttttcccaaa
    14941 gtgatcggtt gaattcccgc ctccgtggca agtcttatct cctccggatc aaatccgccc
    15001 tccgggccga tgaaaatagc gatctgctgt ccgggtcttg cgctttcgat gagctgtttc
    15061 gtctgtggca ttccttccgc cagctcgtaa ggtaccagtt tcagatccat ttcagaagcc
    15121 gttttcaccg cctgcacata actcataacc ggtaccaccg tggggatcac gccacgctta
    15181 ctctgcttgg ctgctgcctc cgcaattccc tgccagcgtt tgatttttgc atccgctttt
    15241 ttctcatcta gcttgaccac gcagcgcttc atggacaccg ggatcacctg aaaggctccc
    15301 agttccacgg ccttctggat gatgagctcc atcttgtccg ccttgggcag tccctggaac
    15361 aggtagatct tcgccggaag ctctactccg tcttccttga taaaacgaag ctccagacgg
    15421 acacagtcct cttccagtgc aaggatccca cagcgatatt ccttcccgtc gattccattg
    15481 ctgacagcca cttcttcgcc ggggcgcatc cgcagtacat ttttgatatg attcacatcg
    15541 gttccccgga tgataacgct cttcgctgcc acgtcgatct gttccggttc tacaaaaaac
    15601 tgatacatag aaactcctat tttaagcact taattctttc ttgcggtaac gctgacccat
    15661 tctccctggt aggtcacttc cagtacttcc aatccagcgg ccttcacagc ctccaccacg
    15721 gtctgctcct tgtcatcaat gatgccagag gtgatgtaga taccgccctt cttcagctga
    15781 tgtacgatga caggagtcaa cggtaccagt acatctgcca gaatattggc caccacgata
    15841 tcataacact catagcctac cttgtcctgc acttctttgt ctgtaatgat attgccgatc
    15901 atcatgtcaa actgtgccgg atcgattcca ttcacttcgc agttctgtgc cacagcttcc
    15961 acagcacaga tatccagatc ggttcccacg gcatgttttg ccccgaacat gagggataaa
    16021 attgccagaa taccgctgcc ggtgccgaca tccaaaagct ctgtctcagg agtgatgaac
    16081 ttacgcagct gacggataca cagctgtgtg gtctcatgca taccggtgcc aaatgccgta
    16141 ccgggatcga tatgaagcac catcttgtca gtatcttccg gtttgatatt ttcccaggaa
    16201 gggatcacca ggatatcatc gatatagaac tggtggaaat actgtttcca gttgttgatc
    16261 cagtcaatgt cctccgtctc atctaccaca acagaagcct cgccgatatc cataaactgg
    16321 cgtaagtctt ccagttcccg ctttacatcc gcaagtacag cttcggtcgt ggtcttctcc
    16381 ccggccactt ccagactgcc gtcctctttt tcctctacaa aaaaacttaa ataggcaatg
    16441 ccgtcatcct ccggtccgtc cggcaggata tctacaaaca tctgctcctt ctccatggcg
    16501 gtcaggggca ccttgtcttc gatctgtgcc ccttccagtc cgagatcgta cagggtgctg
    16561 ataataatat cttccgcttc cgttaccgtc tttattctga atttaaccca cttcattgtt
    16621 ctttttttcc tttcttttcc attgttccaa cacagcatat atcattgcag ccactattgc
    16681 aataaaactg tcgatttcca atacgctgac cgcatccagc ttctctctcc aggtgtaccg
    16741 gttcaatgcc tgtccgtcca cagttgcttc atgtaccacc agttgctgat tttccggtac
    16801 atgcgccttt tccgtagtag tatatactac ctgcggcaga ttctcggaaa tactttgatt
    16861 cacgatccgg tattcataca gggcaccttt tttcaatcgg gtctcaatga ccgccgggaa
    16921 aaaagtcacc ggttccatgg catcataagg aatcacacct tcatatatct tctgatcctc
    16981 tttccatatc tccagaccca gaagtccgtt ccgttcttcc tcctgattat agttcaccgc
    17041 gatttccagt gttttcagaa aatctgtctc cgcaacaaaa tactgcgaag catattctcc
    17101 cacctgcagg ggctctgtat atccataatc ctcacttaag gaactgtcct ttttatcttc
    17161 acggattcct ccatacggcc atacaaacaa catggaaagt atcgctgcca gcaggactcc
    17221 tctttccacc catttaatca tttcccgttt cctccgttat gttcaccttt aaaatatcaa
    17281 aatccgtatt cccatccata tactgaaatc cataatgtcc catattttca taggttacgg
    17341 tatatttatc cgattcctcc agcagatgtc cgaaacactg gaaataccag actcttttcc
    17401 ccaccagttc ttccagatcc ggcacctctt ccatggtata aaagatctgg tccggcatat
    17461 agcatccgaa tagctgtccg aagcgggaat ccgtatcgta gacgaccaca tctcccggct
    17521 ccatattctc cgctataaat gccttcgtct cttccagata tgagattgta ccatattctt
    17581 catgataaga gattccgtag gccgacactc ccatccacac agtgaaggac agacaggcga
    17641 tccattcctg taaattgatc ctggataaaa tgatagccat tcccagccag aacaatccca
    17701 tgctatgcat aacatggcgg taggtaaaaa agtgggacgc gtgtaatgcc agcagcgccg
    17761 ccatcaaata gcttactggg aaaatacagg tacacagaac cgcaaaaaca ccgtttcttc
    17821 tccccagccg cttccaaaag aggatcgcgc agaccagtgt cagtacaaaa agaagttctc
    17881 ccattttggt cgcatacttc actccggttc cgaaaatgaa atcaaaaaat tcgtaaatac
    17941 cgaatttgga ccggttcgtc gggtttccat cagaaaccag cagatgaaag aaatcggatt
    18001 ttatcagcca tgccacaaaa agcaatacaa cggaaccacc acagacaaac ggcttccaga
    18061 tctgttttct gtcctgctgc caaatcaccg ccaccagata caggtagata aacaggtaac
    18121 tgaacgcagt aaagtaatga ctgaaaatac cgcagaaggt cgtcagaaac aatatcgtcc
    18181 acagggcctt actgttcaga cgctcccgca ggaaataagc ggtcagtccg cagacggtaa
    18241 ctccaaacac cgtccaggaa tacattctgt tgttcacact gatcttcatc attgcaggtg
    18301 tcaggcttac catgatcata taaagggctg ctgtcttcgc tccccactgc ctgcggatca
    18361 cgacaggccc cagcagcatg gacaataccg ttcccagcaa tgcaaacatt ttgtaggcaa
    18421 taatgccgtc tcctaccaga aacgaccaca gccgcaatcc catataccat accggcacat
    18481 ccgtatcatg agccgccagt accacgcgaa tgattcccat catcgtattg tcatgggcgg
    18541 tgcgataact gtatgcttca tcatagtcga tcccctcact gagacacagg gagaacaata
    18601 ccgccgtgca cagaatgagg atcagccata agccaatggt tgcaatttta tgtttctttt
    18661 ccgaagtcat ttttttcatc tgcatcaaat cattaatcct gtcttctctt tactcttctc
    18721 tatcatatga ttcagcttgc ggaacggcgg ctccaacgcc agaccaagca gcaatgctac
    18781 gatcaggaac agtgtcattt ttcctacatt gatccaataa tccacgccgt acattcctgc
    18841 aatacattct ttaaacgctt tcatgccaaa ctggaacggc aggaaaggat agatccactg
    18901 gaatacctgc ggaagaacct cgatagggaa cgttccgccg cttccagcca cctggatgac
    18961 catgactaca atagccagtg cctcacccac gtttccaaaa gcgaaagtca cagaatacat
    19021 aaagcagctg aatgccgcac tggccacggc tgccgccagc cagtataaaa acctgtgctg
    19081 acactgtatc tcaatataat aaaggttccc aagaacaata agcagtgtct gtgcctgtcc
    19141 gatcaggaaa aacagcagat atctgccaaa atatctctca tgcgccttat aatgatcccc
    19201 cggttcaatg ggtttcacac ccacgtgaat aatagccacc aggaacagcg ctcccaccca
    19261 gatcgccagg atcgtataga acggcgatgc cgcagagccg taatgatcca ccagataaac
    19321 aggtaccgtc tccagtctga ccggagaaga tacgaagtct gccaccgccg tcggatcggc
    19381 agccatagcg cttaaaatat cctcataggt ctccgcaccg gtgatccgtt ttatattatc
    19441 tgctattttg ctgaccttgt ccagcagttc ctgtgcgatc accagggaat cctgcaggct
    19501 gacattacct tcctgcaaag tctgctgata ctctttcaga atatcggaaa tttcatccat
    19561 attcacatcc gtaccgttca tcatcgctgc ggcattcagg aaagcctgct gcatggattt
    19621 gccggtagcc cggaaagaag gtctgacatt gttattgtaa tcactcatca gattattcag
    19681 ctgtttgttg cagatctgga tctgttcccg gatctgtgcc cgcagagtat tgatatcatg
    19741 atccacatct cccttgctgt cttgcagctg tttcagatcc gcttcgatcg ttttcagaga
    19801 ctggttgatc tcctgaatct gagcatcctg tccggtgatc aggcttccca gcacatccat
    19861 attggcctgc atgccctgta ccagtccgat ggccgtgccc aatccggtgt ccgtggtgct
    19921 tcccacattg ttgctgccca tctgctccaa caatccatca gaaaagaccg acagatactg
    19981 caacgcttcc gtgaccatgt ccatactgct gctcaccata tctcccataa cgtcaaccgc
    20041 atcgacgctg gaattgatca tggtctgcat accgatcatg gtatttcgac cattctccag
    20101 catattatcc atatccggga gcatattctg gctggtctcc atgatcatct ccgcggaggt
    20161 cataatactc tggaaggact gtaacataac cacataatcc tgcaactgtg cctgcatggt
    20221 atccagagat tcttccacac tggtctgccc gccggcattc accaccgaaa gggcatttcc
    20281 cgctttcatc accgcttctg ccacagtact gataaaggtg gcatttacct gttcctgcac
    20341 agcatttttc gctttgcccg tgattttggg ggcaatacca ttctttttct cattttcata
    20401 gtagtaaatg gtgggatgat ccacttcacc gtcgataaat cccagcattt cctgagtaaa
    20461 agtctccgga ataatcaatg ccgcatagta atcactgttc ttcacgcctt ccagtgcttc
    20521 ctccgtggtc tccgggaaca cccagccgat agtggtattg gctcccagag cctccagcac
    20581 actgtctccc acgttcagag acagccccat gatctccgcc ccggcatcat cggaagccac
    20641 ggctactttc agctgactgg tagctgcggg ctcgtaagga tcccagttgg agaaaatgtt
    20701 gaaccaggca tacagtgccg ggaggatgct gagtcccatg ataataacga ccgccaccac
    20761 attattgctg atccggcgga tgtcggtggt aaatattttc cagatattat acatactatt
    20821 caccatcctc ccagtcttcg gtctcgtctt cgttttcctc ttcggtttca tcttccggat
    20881 cctcttcggt cccggtctcg gccggggaat tcaggccttc accaatcagt ttccgggtct
    20941 cccggatgat ctgcgccgcc cggtcaaagt ctgtcggagt caccgctttg agatcgcctt
    21001 cttctccttt gagctccatg atcttctcct gcagattata atccatatat tccacaacga
    21061 tcagatagat tgacagggca aacagcgaca cgatccacag gatcaggaag atgaccttgt
    21121 tgctgccagt caggaacaac agtgccagaa aaaacaaggg gacgatccag atacatttca
    21181 gaccgatctt tattcttttt ctattcttct catgaagggc ctgctcgtaa tccagaacct
    21241 tctgaaaaat ttcttcgtaa tttctctcca tgattttacc tttctgcctg ttcggaactt
    21301 cattcacaaa cccactcctg cggagttttc gttttcatgg ttctgaacag gtgtcacatt
    21361 aactcagtgt cttccatacg ctcttccaca aaatggttca gttttacgaa cgggatccgg
    21421 atcaccaggc cgatcaacag tccctcgatc ataaacaggc tcaattttgc cagtgcttcc
    21481 atataggcag taccgtacat acccgccagt gcctcacgca tggcccggat ggcatagggg
    21541 aagggaaaga aaatataaat cttctggtag gtgtccggta gcagttcgat cgggaaggta
    21601 ccgctggaac cagcgatctg caataccata acaataacgc agacggcctt gcccacatct
    21661 ccgaatgcca gtgtcagaga atagatcaac agggaaaatg ccgtagccgt aaagcttgcc
    21721 gccaggaaaa acagccccgg ttccacaatg tttactttta acagccacag atctccggcc
    21781 acaatgatgg ctgcctgaat ctgtgccaat aagaaaaaca gcaaatatct gccgaaatac
    21841 aattgatagc tctgtacatt ttcaagccct ttcggagagg cttttacttt caccagtgcc
    21901 accaggatcg tagaacctac ccagatcgcc agtgtggaat agaacggtgt catggcagaa
    21961 ccatacgtag ccaccggata catggctatg gtctccatgg ttacgggaga tgcaaaatat
    22021 gcccccagac tatccggatc tcctccaagg aaacgcacca gaatatccat catttcctct
    22081 tcggatgcac cgtccagctt ctctgtcaga tctgtaagtt tggcgctgat attatctacc
    22141 acatctttga tctgtcccag actctcactg gttccggaca cagtgtcacc gacaccttcc
    22201 atgacaactc cgacattttt caccgtattg ctcagggtat ttaccagatt ggtcacctga
    22261 ttcagagaat ctgacatatt tgtcagaacc ttctgcatct ggggcaccag attattctta
    22321 tagattccct gcatattact tacagaagtc tcgcagctgt caatggcgct ccttaaatta
    22381 ttcatggcat tgttgacatt gacctttgca ttctccgcag ccttgtcgtc cgtggattcc
    22441 agaacgctgc ccactacggt attcatcaga tccagttcct tctgcatggc tttcaatgct
    22501 tccatagccg catcagttgc cgctgcgttg atattgctgc cggtgccgcc gttcgcagca
    22561 ttaccgtcag agacgctttc cagcttttgt tggatcagtg ccgccagcag tgtatttact
    22621 tgtccattca gcgtattggt atctcttgcc acccggttca gatctctcgt catctgggcg
    22681 gtatcctccg ccagtttgct ttcatccagc gcctttttga tactttccag agagttattg
    22741 atattgctca tggaagtatc gatgttgttg ctgaaattgc ccagggtgtc tctggtagaa
    22801 gacagatcct gactgctctg gttcagactg gctgtggtgc tgtccagttt cttctgcatc
    22861 gcgggaatct catagttgac atgggtgatg gcagactcca gccgcgcatt gcctgcctga
    22921 aaggagtcga tcatggcact gtattccttc agattgtcat tcagttcctc cagtttggaa
    22981 cagaattcct ctacataact gtccccctgt acctgatccg acaccgtatt gccctcttca
    23041 aataccgtgg tagccgccac cttgataaag gtctcattga tggtattctg cagagttcct
    23101 gccaccgtat ccgtgatttt ggtagcaatg gcattgctct tctgattctg ataatagatc
    23161 agcgccggat tctcaaaatc atccgaaaat acattgtaca tactataagt aaatttatcg
    23221 gtaatgacga cggctgcata atattttccg gcttccacgc cctcttttgc ttcttcctcg
    23281 gtatccagaa agatccattt tacactggtg ttggaatgca ggctttccag tacggattct
    23341 cccatattta ccaccgatcc gtctttcagc gtatagtcct gatccaggga tactaccgcc
    23401 acaggaattc ctccggtatt ggcataggga tcccagttgg caaatatatt aaaccacgca
    23461 tacagcgacg gtatcacgca caacccgata ataataacca gcgcaaaagg ctgcttcacc
    23521 agttttttca gatctccggt aaaaatcttc caaatatttt tcatatagct tctaacaact
    23581 cctcatccgt atccatttga ttggttttgg tcttttctcc ccgggccgcc gcttcacaca
    23641 atcccagtat caggaagaaa agcggcgcat tcagcacctg ctgaaagctg atcagggaat
    23701 gtatcccata ggtaagcagt aacaatactg cgagaaaatt cttcctgtat gtctgcagcg
    23761 ccgtgataaa aacagctgca taggcagcca cacccagcag tcccatattg accagtgtgg
    23821 tgagccactc attatgtgca ttggtaaaaa cagagccttc aaaatatcct ttatcaaaaa
    23881 gcaccgtgcc tcccggcaac agttctccca gatacgatgc aaaacaatcc gggcctgctc
    23941 ccaacagctt ctgatgcaga tttcctttcc ggaaacccat ccatgccatc tgccacaatc
    24001 ttccccggcg attgccccat tccgcaaaat cgattaccga caaccgatgc agataccatg
    24061 ccagcactac cgctattcct gccagtgcac aacacaatac tccctcttgc aatatttttt
    24121 tcttattctc cggaatcctg tgacaggctc cccaaagcag cagacaccct gctgcaaccg
    24181 gaatccatcc ctgccaggct gccagttttt tggcggtgcc atcctgcagg agaatgtcaa
    24241 acgttcccag atataatatc catttacccc aacagcacat tcccagcgcc accgctgcaa
    24301 gaaaaaggaa atatacttcc caatgtccgg tttgcttccg gtcgagccaa aagcacaacc
    24361 ccatgactgt gcatgccacg atcacaccgc tgtcacttcc ctgtaaaagc agaagcatca
    24421 tggccaacat attacacgtt cctgccagca gtgtgggacc ttttttcccc gcttccactg
    24481 cccggtgaaa cagcgccatg gaaaagggaa acatcacact gtaatacccg ctgagccagt
    24541 tgctgttccc tagcgtggtc aacatatggg taaactccca gtcccccaca acgtaaccct
    24601 tgagaagtcc cagaggatca tagcccagct tctgcaacag accgatcagg gtgaccgcca
    24661 caaaagccgc ctctcctaac catatcgccg ttttacaact acctccgtac ttcgccgtca
    24721 gtaaaaaacc tccgatcatc aaacagatcg tgacagctcc catgtaccat tcccgctctc
    24781 cgttccacgc gatactgcca taggggctgc agattgcaga gagtaacgca cagattccgt
    24841 acaggcagac cgccgtcgtt accgcatgtg ttctgcattt atcgaccgat tttttcacta
    24901 cttcagccag agatctggca aaaatccggt gttcccctgt ccaccggctg cttacagtaa
    24961 agacacacat tgccagcaat gcgatcccct ggcaaagcag tgaaacattg cgatacaggt
    25021 aatatttggt atcacccagt ttataatatc cattccaggg aatataataa agaggcagca
    25081 ctgccagaat aacgatcaga taactctgac atacaatttt cagaaattcc tctttccggt
    25141 tctccatcct gcaggctcct tcgtgtcaca gacagcataa ctacttgaat tgtaagcgtt
    25201 tctgcccatt ctgtcaaacc gttcaccctg ccttttgaca aaaaaaccaa actgtattct
    25261 atatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctgtc aaaccgttca
    25381 ccctgccttt tgacaaaaaa accaaactgt attctatata aaatactcat gcctttcagt
    25441 aacctgcaaa ctttgagccg caggcacaaa gttgcgtgtg aaaaatttat ttctcacatt
    25501 attttccttc aaggatacct ttaacttttt cctaacaaac cagtagcccc ggctgggtgt
    25561 aagaataagc agtaccgcta cgctctgcga tagagcgcga gcgtgccgcg tttcttaccc
    25621 cagccgaggc tacggggaat tttatttatt aacttttact tccaaaactt ctttttcttt
    25681 ccgtccttgg gatccttctc cttggtctcg ccttctccct gtccggtggc attcttcacc
    25741 gcattcaggg aatccccggt cagcgcatca aactgcttta acagttcctt tgcttccggc
    25801 tttagcttat cgggggtctg aaccaccagt gttacataat ggtcaccgcg gacatcctta
    25861 tttctccagg aaggaacacc cttgccgcgc agacgaatct tggtgtcggt ctgtgttccg
    25921 gcctttacat catataccac cttgccgtct accgtatcga tcagcacttc gccgcccaat
    25981 gccgctactg caaaagaaat aggtacggta gagaaaatat cgaaatcctg tctctggaag
    26041 ataggatgac gacctacgat cacttctacc agcacatcgc ctctggggcc accgttgaca
    26101 cccggctcac ccagtcccgg catacgggta gactgtccgt tgtcgatacc tgcgggaata
    26161 tccaccgcat aacgcttctt cataggaata tatccggtac cacggcaatc cgggcacttc
    26221 tccttgatca ccttaccggt accctgacag tcgggacagg tctgtacatt acgcacagta
    26281 ccgaagaaag actgctgggt aaataccacc tgacccttac caccacactt ggtacaggtc
    26341 tcagggctgg ttccgggctt cgcaccggag ccgttacagg tcttacaggt ttcttttacc
    26401 gtcaggtcaa tttctttctt acagccaaaa acagcttctt caaatgtaat atgcacgctg
    26461 gtacggatat tggcgccctt cataggtcca ttatttctgg cacctctgct tcttccgcct
    26521 ccgaaaatgt ctccgaaaat atctccgaag atgtctccga agtctgcacc gttaaagtcg
    26581 aaaccaccgg cgccgcctgc accaccgtca aatgccgcat gaccatactg atcatactgg
    26641 cgtctcttct cgggatcact cagcacggca taagcctcgg aagcttcttt aaacttctgt
    26701 tctgcctcct tgtctccggg gttcatgtcg ggatggtatt ttttcgccag tacccggtat
    26761 gcttttttaa ttgcagcatc atctgcattc ttatctacac cgaggacctc gtaatagtct
    26821 ctcttctgct ctgccatttt tgcacctctg tcatttaaat ctaaaaacgg ttatgcacag
    26881 ggcacgcttc cgcataccct gtgccacctg ttattcgcaa tcactgttac acagcattta
    26941 ctgttatacc acgatttgcg ttatttgtga agtttaatta tacttctctg aaatctccgt
    27001 cgatcacatc atcgtctgcg ttagatccag cattgctacc ggcctgtgca ttcatgtcag
    27061 ggcctgctgc accctgtgca ccctgctgtg cctgagcagc ctgctcatac atcttggtga
    27121 atacagactg agcactctgg gtcagtttct cttttgcagc cttcagctca tctacatcgc
    27181 tgtcgctcat ctcctggtct ttggttctct ccagaatagc cttcacagct tccagatcag
    27241 cctgtacctg ggtcttgtca ctctcgggaa ccttgtcacc tacttcattc agagccttct
    27301 ccgtctggaa tacgaaggaa tctgcttcgt ttcttgcctc gatagcttcc ttacgcttct
    27361 tatcctgtgc ttcgaattca gctgcttcct tgacagcctt ctcgatatcg gaatcggaca
    27421 tgttagagcc tgcggtaatg gtgatgtgct gctccttgcc ggtacccaga tccttagcgg
    27481 atacatttac aataccgttg gcatcgatat cgaaggtaac ctcgatctga gggattcctc
    27541 tgggagcggg agcaataccg tccagacgga actgtccgag ggacttgtta tccttggcaa
    27601 actgacgctc accctgtact acgttgatat ctacagcggt ctggttatca gctgcggtag
    27661 agaagatctg gctctttttg gtaggaatgg tagtatttct ctcgatcagt ctggtagcga
    27721 caccacccat ggtctcgatg gacagggaca ggggcgttac atccagtaac agaatatcac
    27781 cggcaccagc atcacctgca agcttaccac cctggataga agcaccgatt gctacgcact
    27841 catcagggtt cagagatttg ctgggctctt taccggtcag ggctcttacc ttatcctgta
    27901 ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttcagag atttgctggg
    28021 ctctttaccg gtcagggctc ttaccttatc ctgtaccgca ggaatacgtg tagaaccacc
    28081 taccagcagt acctgaccca gatcagaata gttcaggcca gcatccttca tagcgttctg
    28141 tacggggata gcagtcttat caaccagatc tctggtcagt tcatcgaact tagcacgggt
    28201 taaattcata tcaaagtgct taggtccctc tgcagttgcg gtaatgaaag gaaggttgat
    28261 attggtggtc attgcgctgg acagctcttt cttagccttc tcagctgctt ccttcagtct
    28321 ctgcatagcc atcttatcac cggacagatc tacgccttct gccttcttga attcatccag
    28381 catccactga atgatcttgt tatcgaaatc atcaccgcca aggtgtgtat caccgttggt
    28441 agccagtact tcgatgacac cgtcaccgat ctcgatgata gatacatcaa aggtaccgcc
    28501 accaaggtcg tataccatga tcttctgttc tttttcattg tccagaccat aagcaagtgc
    28561 tgctgcggta ggctcgttga tgatacgctt tacatccagg cctgcgatct tacctgcatc
    28621 cttggtagcc tgacgctggg catcattgaa gtatgcggga actgtgataa ctgcctcggt
    28681 aaccttctca cccagatagc tctcggcatc tgctttcagc ttctgcagga tcattgcgga
    28741 gatctgctga ggagagtatt ttttgccgtc aatggtacga ccgcgatcgg tacccatatc
    28801 tctcttaatg gaagaaatgg tcttctctgc gttggtaact gcctgacgct ttgcaggctc
    28861 accgaccagt ctctcaccgg tcttggtaaa tgctacaaca gagggtgtcg ttcttgcacc
    28921 ttctgtatta gcgataacgg tgggtttacc accttccata acagctacac agctgttggt
    28981 tgtacctaag tcaataccaa taatcttact cataatattt tcctcctgtt tttattctct
    29041 gttttcattt tatagtttct attctcaaaa atatgtgata atcaaattat tgaacaacgc
    29101 ctaccatgct gtgtcttacg accatgtcat gatatgtgta acccttctga agttcctggg
    29161 ctatcacacc ggattcatac tcatcgctct ccacctgcat cactgcgttg tgaagattcg
    29221 ggtcgaattc ctgtccgact gcctcgattg gtttcacacc gatgttctca agttctgtca
    29281 tcagctgttt gtagatacgg ttcataccgt ctacgaaagg atcctctttg gattcctcgg
    29341 gaaccgttgc caggcctctt tcaaaattat ctactaccgg aaggatcttc tcgatcacac
    29401 tcttggcacc ggtatcgaac attgcctgtt tctctttatc ggttctcttg cggaagttct
    29461 cgaactctgc catctgacgc tttactctgt cttccagctg atcgatcttc tccttgtagg
    29521 aatcttcctt tttgttcagc tttgcctgct tctttgctgc ctttttgtct tccttgctca
    29581 cgggttcctc atcttctgtc tcttcttcct tcagttcttc ggcatctgaa agatcctgtt
    29641 ccatttcttc ctctaatatt tctttttcct gatctgccat tcttactttc ctttctggaa
    29701 tctgtctatt ctttgcggta aaggtcatcc aactggttct gcaccgtccg cagcgttcct
    29761 atgaccttgt cataatccat tctcttggga cctatgattc ctatggttcc cttcatgcct
    29821 tctcccaatt cataagtagc tgtgacaaca ctgcaatcct tcatgccctg taccggtgtc
    29881 tcgtctccga tgtacacctg aatacctgtg ctgccttcat ctgccaggga ctcctggatc
    29941 agaccgccca gctgctgctt ctcctcgaaa gcattgatca gttcacttgc cttggactga
    30001 tctgccagct ccggataccg gaaaatattg ttggcaccgc tggtgtagat ctccaggtcc
    30061 tcgtctgcct tgatggcatc cgccacagca tcgatcacct cactgacgat ctcactgtgg
    30121 attccggcct gctgcttcat tgctgcgatc attcccagat tgatctcctc catggacaat
    30181 ccattgaggt gggtattcaa cagaatgttc agcttcagca gtgtctcatc atccagttcc
    30241 tgattcaccg ccaggatatt gttcttgatc acattacctt cggcaacgat gaccgccaga
    30301 atctgtccgg catctacccg ggacagctgg atgaacttta ccttgttgcg atgtacctgc
    30361 ggagcactga tcatggtggc gtattgtgtg ttctgagcca gggtcttcac cacctgtttt
    30421 aacaggttct ccagcttatc ctgcctctct aagagcatat ccttcatctc attgacttcc
    30481 tgctctctgg tggccatcat ggtatccaca tagaagcgat atcccttatc ggaaggaatt
    30541 cttcctgcgg aagtgtgcgg ctgtatgata taacccatct cttccagatc cgccatctca
    30601 ttgcgaatgg tcgccgagct taaattcaga tctgtgtatt ttgaaatggt cctgctgcct
    30661 accggctctc ccgtttccaa gtagttccgg ataatggctt gcaatataat tgtttttctg
    30721 tcatccaatt gcattccatc actccctttg ggattattag cactcatcta aaatgagtgc
    30781 taacatcttc ttaccctaaa atagcactac agggtattat tgtcaactga ttttccggaa
    30841 aataattcta aacaaactat taaaaaaaac tattcagagc ctcattcgcc aaatacagat
    30901 tcttgtgcaa gcacaaatct gtacttggct catcgta
//
LOCUS       DAWEJW010000020        30587 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_634_length_30587_cov_63.689703, whole genome shotgun sequence.
ACCESSION   DAWEJW010000020 DAWEJW010000000
VERSION     DAWEJW010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 30587)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 30587)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..30587
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_634_length_30587_cov_63.689703"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    16191..16290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    24060..24159
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7062 a         8411 c         5860 g         9054 t
ORIGIN      
        1 cggcaactct gatatcttat cataaccgct ttcgtttgtc aacaactttt ttcgattttt
       61 tcaaatcttt ttctcaaccg gaaaactttc attttcgcag tggagtttca tactaccaca
      121 ttgctccgtg ctttgtcaac aactttttac atttattttt caaagcattt acacaatggt
      181 ggaaatggcg taaaaaaaga aatctcaatc tcttaagatt cttcttttac ttcctattgg
      241 tagtaaacgg agaaagaggg atttgaaccc tcgcgccggt tacccgacct acacccttag
      301 caggggcgcc tcttcagcct cttgagtatt tctccatgaa ctgaacatcg tctctaccaa
      361 tatgttattg gcgtcatttc gtgacgcaaa aattattata cataagctct atgcttttgt
      421 caatcgcttt ttttattatt ttcctaattg tagatattgt ttatgaatat tgccgaatag
      481 cttcttcaat cttcgttttt acaatgtccg caatctccgg tgttttcaac tgtttatact
      541 cttcataaca gattggcggt aaatagatca gctttaccgt aaccggagct atggacttct
      601 catcaaacgg tttgtaagaa tcaatcagag cacatggtac aatggggcac tttgctctga
      661 ctgcgctttt gaatgttcct cccttaaatg gaagcagttg atttcccata cggcttctgg
      721 ttccttccgg gaagatcaga aagtttctgc cctgctttac ttcttctgtc atctgattga
      781 tcacctgaag cgattgctta atgtcttctc tgtctatggc aatcgcgtgc aatgcccgaa
      841 atacttgttt taacaggatt acattgctta cttctttctt ataaactaca gagaaaggaa
      901 ccgggcagga ctccagaaac acaagtgcat caaacattcc ctgatgattt gggaaaaaga
      961 taaacccgtt ttctttcgga atattttcca caccatagct ctctatggtt actcttccgg
     1021 cacggtttgc cgcttttgtt acctttttga taaatgcata gttcttttcc aggtcaaaat
     1081 tctttctggt tcccagccac catatccgaa tcagataata cggcacctta tagaacagtc
     1141 ttaccaccat cagtatgatt ctatacatag tccgccccag tctactcttc gttatattct
     1201 tttaccgccg tttcatacag gttattacct tctgcgtcaa ttactacaat cgcaggaaag
     1261 tcctttacgg tcagtttacg gatagcttct gttccaagat catcatatgc aatgacctca
     1321 gaagaagtaa ttgcgcggga caaaagtgct cctgcaccac ctacggctgc aaagtatacc
     1381 gctccgtttc ttacaatggc atcccggact tcctgtgacc tttttccttt tccgatcatc
     1441 cccttcagtc ccagatccaa taatcccggt gcatatttat ccattctgct ggctgttgtc
     1501 gggcctgcag accctatagg acggccttct ctcgccggag acggtcccat atagtagatt
     1561 acgttatttt tcattcctat aggaagtgct tctcccctgt ccagagcttc ctgcattctc
     1621 ttgtgcgccg catcccgggc tgtatagatg gtccctgtca gatatacata atctcctgat
     1681 ttcagagagc gggcatcttc atcgctgaga ggtactttta catatttatc catgtcgctt
     1741 acactccaat tattataata tccggacatc tttacttcag atctctctaa ctgcatgcct
     1801 gttgacatgg caacagatgt ttacagctac cggcaatccc gctatatgtg tgggatatgt
     1861 actaatattg accgccagtg ctgtggttga tccgcccagt cctcccggtc cgatgcctgt
     1921 tttattaatc ttctccagta gctcttcttc cagttccctc acatagggta tctccgaatg
     1981 ttcatccacc ggacgtgtca atgccttctt cgccatcaat gcacattttt caaaagtacc
     2041 gccaatgccc actccgacta ccataggagg gcacgcattc ggtcctgcat cctttaccgc
     2101 cgtcaggatt gcgttcttca cgccttctat tccgtctgct ggcttgagca taaatactct
     2161 gctcatattc tcactgccaa atcccttcgg tgccacggtt atcctgacac gatcccccgg
     2221 aacaatacta taatgaatga tcgcgggtgt attatccttc gtattctccc ggtaaatggg
     2281 atccttcacc acagattttc gcagataacc gtccacatat ccctgtctga caccttcgtt
     2341 gacggcatct tccacattgc cccctgtcag atgcacatcc tgacccactt ccagaaatac
     2401 aaccgccatt ccggtatcct gacagatggg aatcatatcc ttgcctgcaa tatccatatt
     2461 ctgctgcaac tgttgcagga tctgcttccc caaaggtgct ttttcctgct gttccgcttt
     2521 tgtaaatgcc gtcttcatgt cctcagataa gaaatgattg gcctctatgc acatttcttt
     2581 gatattttgc gtcaaagtag ctacatctac tgttctcatg gtttctccct gttattcggt
     2641 aatggttttg cgaatctctt ccagttcttc cggtgtactt tccccgggat tccataaaat
     2701 atgctgtcca tcattctcat aacggggaat caaatgcata tgaaaatggc gaacggtctg
     2761 tccggcagcc tctccattat tctgtacgat attgagacca tcgcaatgta atttctctgt
     2821 catctttgtt gccatctttt ttgcaagaac aaatactttt gccgctgtat cctccggcag
     2881 ttcatagaga ttatccgcat gctccctggg caggatcagc gcatgtcctc tggtagccgg
     2941 tcccagatcc aagattactc tgaaatcccc gtcttcgtat aatgtcttgg aagggatctc
     3001 tccgtttgca attttacaga aaatacaatc ttcctttttc atcgttttgc tcctatctgt
     3061 tattttcttc aaagggaaac atctgatcca gtgttctgag aattcggaaa tctcccttca
     3121 ttcgcttcat aatgcctgac ataaacgccc tctggaaggt cattcgtccg ttcacaatat
     3181 cttccatggc agcacgattc atctgcatct ccacatccgg cttctccgtc tcaccgtatc
     3241 cgcattccag tgcgccactg ccaatagcga tccacagagg attcttcttt tcttctatat
     3301 taataacata tcttgcttca aaaccaggct gcggtacaaa aacttttcgg aactcttcca
     3361 gatattcctg ttcattatcc gttccgctgt tatccaacat atcccggaac atggaagtca
     3421 attcctgaat atcttccttc tgtcgctgta caaacgtatc atccgataca tattccgaca
     3481 actgttctgt ctcctgcggt gtcaggttga ttgccttggg gatcgttact ttctgacgca
     3541 tcactttatt gctggccggg aaactggcaa tcttctggtt gatagtacgg tacatgttct
     3601 cagccttttt ctcaatgatc tggccgtact gtccgttcat ctccaacgtt actgtattct
     3661 ctatatatcc gcatatgccg ctgcagggca atcctcccag aatctcccag gccgccgcaa
     3721 gactgagctt gccttctctc tctccgtagg tggtagacat cacgaccgga cacatataaa
     3781 ttcccgcgat cgtctcttta tccccataca gccagcaggc atccagaaac tgctgcatat
     3841 agccaccgat accgtaccac tccactgtcg ttgccagaat aattccgtct gcctctttca
     3901 gcgtctgtgg caatgtcgta atattggtct ttccatcata aagattgtat ctctccacat
     3961 gcacccgcaa ctcttccagc acttcctgca tcttattgat cacatagatc gtaggatcat
     4021 caatgattcc tcgtccgcca tagtaaatgt tgatattcaa gtccacacct ctttcgtttt
     4081 cttctgtctt ctgtgtttcc tgagcgcaca aaacaaaagt cccgccagga atccggtcac
     4141 cagtccgccc acatgtgccg cattgtcaat attttgtgca gtaaaaccac tgtacagaga
     4201 taatgctatc atcagaatca cccgtttggg tgtcacatcc ggcatagatt gccgatccag
     4261 caaaatccag gcaagcagta ctccgtccag tccaaagatg gctccgctgg cacctacaga
     4321 tgccgaataa tccatctgca gaagctttac cagcagtgag cataaattcc ctccgattcc
     4381 ggacagaaaa taaagcacca gaaacgtgac atgacctgtc accttctcaa tcatggcccc
     4441 cagaaaaaac aggaggacca tattattaaa aatatgctct atgcccgcgt gcaaaaacat
     4501 tgcccacaaa agcctgccgt actgtttacc ggccaataca ctgactacat ccagttcgcc
     4561 tttattatat aacacatttc ccgaaaaagt acacatcagg aacacaatta cattgatcac
     4621 cacaagcatt ccgcttaccc agggtacatt acgaagcctt ttcaccttca ttgctcctct
     4681 caatgttctc tccatccggt attgtatttc aaatgtacca ctgtttatat cactccgtca
     4741 acaaaaaggg ggctgcacac tgcctaccgg tagattaccg tgatcttacc caatgtaatc
     4801 tcatctcctt cttccaactt tcgcctctca taaggctgca gttgtaatcc gttcttaaat
     4861 gtaccgtttg tggaattcat atcttcaaga taatacccat ctgtttcctt tatgatcctt
     4921 gcatgcagac gactgatcga ctgatcattc aataccagat ccgtttctcc ccgttttttt
     4981 ccgatcgtgt agattggttg ttctaatgtt gcaagcagct tcccttcttc cgacaacagg
     5041 cggtgtgtac tctctctggg ggccggtctt tcctccatat acacagtcct tcccatatct
     5101 tcttcctcat aagcggtctc ctccgccacc gcatatcccg tcatggtcag ttgcagactc
     5161 cgggtataat tatcccgttc ctgcttctct ttctttttcc ggttttccca cagattaaac
     5221 aggcctttct tttcctttat tactgtaact tctcccaccg gcatttcctt ctcttcttcc
     5281 cttatcgcat ccttctcctg atcttgtccc acgacaacag ttgtttcctc ctctacggca
     5341 tccatttttt cagggattct caggcactct gcatcctcaa aaattttggc ctgcaaatag
     5401 acctctcctg ccgattcata ttgctcgtag gctttataga catattccac caggctttca
     5461 tcctgataat ccacatgttc caccaggtat tccatcagct catcaaatcc tgtattttcc
     5521 gtgcaatacg ggacatatat aaaataaaat tctttcttct ccagatcctg atagacatgt
     5581 tgcggaaacc acacgatgtt actctcttcc aaaaggaaac gggacatttc ctgccgcact
     5641 ctgcgcatac tccacaaaaa gtccatgatc cattgtcttg taataggctt cttctcaaaa
     5701 agctgtgcaa tattctgtct ggaagtaata tcataataga gataggcctc tccgtctatg
     5761 tatctcaaac tgcagggcag caatcctttg atccctcccc ggctgatcat acaatactga
     5821 tatttctttt cctccggttt ccgttctaat aatagtctct cataattcgc atttaagctt
     5881 ctgatatatt ctgtatttag caactattct tccatccttt ctcactgtac cgcaccactt
     5941 gtattttcct tgttatcact ctctgttgtt tcttccgtct tcccacgata cctctgactg
     6001 tttcgcagaa tctccaccgg attgttcctc ttcacctgaa atgcggcttt tgcgctccaa
     6061 tacctgggta cttccgctat ttcggaaaac ggataaggca ttcttcccgt ctgttccacg
     6121 gttacctgat tcttttccag tttccagacc ggcaattcac tctcccaggc aatatatttc
     6181 tccgtgtcaa aaaacatatc tttctcctgc agctgtcgat tcagttcctc ttttttctgc
     6241 atccatctac tgccgcttct cactgccgcc cttcccatat cctgttccaa aaggcagcga
     6301 tcatattgaa aaaacagtaa atacgtcatc aacactatca ctcccaacac tataggatac
     6361 agaactgccg cctccacggt aaaatacgct ttataattct ttcgttgcat acgattgctc
     6421 cctccaatca tcctgtcatc catgccgtta tatatgctgc cagtaaaaag ggaagatatg
     6481 ggatctgcag gctgcgattc cgctttctgc aaaacaggat cattgcataa agaaagatat
     6541 acagaaggct tatgcctaag ataagaagaa tatttttatc tctgcttata aaaccggtca
     6601 gcgccagtac gataccatcc gcctcaccga tttcctttgt tgcctttgac aaaagcaaca
     6661 gcagaattcc gggaagtagt gcgacaagcc tgctgcattc aaagcctttc cagacaataa
     6721 ccataacagc cagtactccg cccaggaaca atacccccac cggaaccgct ctcttccgga
     6781 tatcatacca gctgccccat agcagatagc cggtcagtat cacattttcc cacaactcca
     6841 tcttcaccat tctccagttt tatattcttc cgcatctact gcattctctg tacccgtcca
     6901 catctttcct ggcaatgcaa tatacggttc tcttaagccc ggggcagtcc cgccttctgt
     6961 gataacattc accctccgag caaatatagt agccattctc ttccggggca gcggcatctt
     7021 ttttctgtac catacacttc tcacagggat gataaccctt aggaatttcc gctgcttcca
     7081 cccgtcgaac cgacaatctc agatgtgtac attctctgct tctatgatat acttctgaat
     7141 cttccgttac atagacatat tcttcatcat tctcagtccc cggaatcgca tagccgttcc
     7201 acaaatgccc atagtatcgg ttcgccatac ggaattttcg aaatcccaaa acctccgtga
     7261 gcggactgat cccataggtg actacaatct cacagatatc attctttgtg ctgctctcca
     7321 gaaattgcag gctgtctgcc ccctgttcta atggcgactg ctccagatat tcttctccca
     7381 gctgattgca gatctgattt ttaacatagg tataggaaac agccactgtt cccaacctgt
     7441 catcgttatc ctctttctct ccggcatctc ctgcattcag tattccgcca tagattccca
     7501 cctgtctgcc ggcatcccac aatgctactt ccaggtttcc atgcaaacgc atcatttcta
     7561 tagcgcttcc catatggatc ataaaaaagc aaaacaacgg caaaaccagt gctgtttcca
     7621 ccgtcatact tgccctcacc ggtgttcgcg ggagtataag cagtgatatc ctctttatga
     7681 agtttgctgt ctgtaagaat tttacgggga gtaaatcgtt acatgggtta agagtttttc
     7741 ttaaagtcgg agtaggactt gattcattct tgttaatagt tataaagctg tacttgcata
     7801 aagaggacat cgctgctcac ctcccttccc ggctcgtctt cctaattacc agtattttct
     7861 attgcctcta aagatcctat ataagatttc tgtctctcga tctcaaaaga atagccatat
     7921 ttgcttttta ctttcacatt cattgcaatt tccacaatgc aggtatccag tcggaatgct
     7981 ctgtttcccg gagtatttcg aatatccatt tccaccacgt ccatagcccg gcaggtaaca
     8041 gactgttcct cacagagcag caaaaatatt ctcagataat cttcatatcc cattcccggt
     8101 gaatcggaac cggaggcatc ctcttcggaa gtcccggaca attctccctg taatgcggaa
     8161 gaaagtccgt aatgccaggt agcatctgtt tttaacaggg gaatcttttc cccggcaaga
     8221 agttccttta catcataaac actttctgca aaagcccatc ccaggatcag tgtcgccgca
     8281 agcaactctc caatctccgg aaccatcatt gccgctgcaa gtgcctctcc caatagctcc
     8341 gctattgccc gtttttcctc actaccggtc agatacatgg tatttgcagc ttctctgata
     8401 acgaatattc ggttcactat tcccttcaga ttgtcagtat cattatcctt gcctacaatg
     8461 acatattctg tctgatacca tagggggctg tcctgtttct cctggccata ccgcccaaga
     8521 taccgcaaca gatactcatg gaaaaaaact ttttgtttta acgcttccca ttcttcataa
     8581 ttttctgcgg tccccggcac cagatttccg cggtttattt ctcctcgctc catccgcgtc
     8641 tccaatagtg actctgtttc tatcctgcgg gaggagatat tgtcttcctc tgttaccagc
     8701 tttaacagac ctttatttct tttctcttcc agggctgcgg taggattctc tatatgaacg
     8761 gtttttcctt ccgtggtctc cttcccgtca tattcctgaa tctgtgcatc gatctgctgt
     8821 ttttgtcccg caatatcctg ttgttccagt ccacgggatt ctatgacctg cagccagtcc
     8881 gtcagttgct gtaagagtcc gattcccacc gtatcctcta tggcatcgac agcaacccgc
     8941 cgaaagactt ctccttcgcc atccgtcaga aatgccgctt tcacagcctg tgcttcttct
     9001 gcctgaacag caaaaaaatc ccggtacaac agtttttcga gaaacacatc ctccagagaa
     9061 aaattacggt tcatatactc cagcaaatga ctctccgtca gctttgtcga agcactttct
     9121 gtcccgtaag agcagtctat ggcaaacaga ttgtactgtt tctgcagttc tctatgatat
     9181 tcagccagga tactatccat tccgatatcc atgacgcatt ctgtttccag tgagattccc
     9241 cgataccgac agccctcgat caaagcaaga cacagggcca tgatcaccgt caccgtcagt
     9301 gccagatata ccgtcatata accacccggg gttacctttt tcacggatgt cataacgtat
     9361 tttccttata tctttttggt ctgatttgta atctttgtga aaatggaatt gacaacttcc
     9421 gtgatcttct ctttaaaaat aatgaccagc cctaccagta ccacaatgat tagtattact
     9481 tccactgtac ccattccgtc ttcttctgtc aggaactgtc gaaatgattt taatttgttg
     9541 tttcccataa ccataccttc cttttcttat attcccgcat tcgaaaatgc aggcaccatg
     9601 atcattacca ttactaccac cagcagcatc accataggca ggagcagttt tgtcgttgct
     9661 tcctcactga gttttctggc attctgcatc cgcagttctt ctgcctgggc agcctcttcc
     9721 tttaattgct gcaaaagcgt agaactgccc tttttcagat tttgtgtcag gaatgtgctc
     9781 aaacggacat attccctgac tcctgtcctt ttcccaaagc tctcatatgc tgccccttcc
     9841 ggcactccca tatgtatctc ccgggttgct atcagcattt cctcgtatgc ttcccggcaa
     9901 cgtccccctc gttttctttc cttctcgtaa tcttctgcaa cccggcaaaa cgcagaccgg
     9961 accgtcaatc cagcttccag atataacgtc agcttttgca gaatttccgg atagcttcta
    10021 cgttcctctc gctttttctt ctccaccaga tcatgcagat ccttgtcctg gaagaaaaag
    10081 actgctgccg ccaccaacag agcccccgcc aggatcttcc atccagtctt tggaatatct
    10141 tcttcccagg aaatttttct gccctcaatt tctgtgggca gctggatttt ttcactttcc
    10201 ggatcttcct cctgcgcccg ctgcagtaat tccgacaact tgcttttctc tctttcctgt
    10261 ggggtctcat tcctttttac gactgttacc ttaaccattg tctctctgac aaattccccg
    10321 tattgtatct ccgctctcaa caatacaggc acatcctcct gcccgggatg tatctgtcca
    10381 ttctcttcaa cagcttccgt agaattgctt ttccagttca ccagaaacgg gtatccttca
    10441 tacgtttccg ataaatccag attctctctg atctcatcgg gagaatcatt tttctccagg
    10501 atcaactgag gcagttctct cccaaactgc tcgtacaaat tctccaattc gctttggcta
    10561 tacttgcgag gcatcacttc catctggaaa gaatatctct cgccgtcctc cacctgtccg
    10621 gtgatgctgt attcccgact gccctcttcc ccagataccc gatacaccca ttgattctgc
    10681 aggatatgac tttcccgact gctgtaatgc attgtaatgg caagcattat ccctgcgatc
    10741 agtatgactg cagaaagtgt cagctttgtc acataatagt ctgtcacaca ctcctgcgga
    10801 ttttctcccg ggtgcatacg attcagatcc gcttgcacct tatctcttcc aggtatcgga
    10861 attctccaca aatgtagctt tttatagcag accatcatca ccttatagaa aaaacgcagg
    10921 atacctttct tacctatcat ctctttctac agttcctcct cgatccgatc cagtatcttc
    10981 tcacccagca gatatgctcc tgcatagact gcaaggcaga gtgtcatcac tatgataccc
    11041 attccattat ggtagagcgt tccaaaatat cccggataag cggctgcgat atagagagaa
    11101 attccaaacg gcatgatctc catcacgttc tgttccagtc ttctcccctg taacatggtc
    11161 agcatctcct gcatggtttc cactttatta ctgatcacct ctgctgtagt ccgtatgatc
    11221 tgcgtgacat ttccaccgcc tctcttggca atgttcaaaa tagcggaaaa ttgctcaatt
    11281 tcctcaatgc cactccggtc tgccagatca ctgatcaact cttccagcgt gatattcagt
    11341 atcattcctc tgcggatcag ttccagttcc cggtacatgg cggaatgctc accatacaac
    11401 attcgtatat cctcccggct ttccagaaaa gcattttcca acgcatatcc gcttctcaga
    11461 gaatttgcca cagacaagat gcattccttg aattgcatgg tcagttcctg tctgttcttc
    11521 cgacactgtc tgttgacgca cctgcgaaaa tagagaatcc ccagagggaa caataacgga
    11581 accgcccaga tagagcgata gaaaaaccag gcaaaaaata taactacccc tgatgtctgt
    11641 gccaacagcc gtatgcaatc tccccgcttc tgaaaaacct tatgataatc ctgctgcctt
    11701 aagctttcct tcatgaagta attctccttt tctccgcagc ctgccgacca cttttccttc
    11761 actgttttcc cccagctctt caaactgata caaaggattc aggcgtattt ctccattctc
    11821 acaacctact acctccgtga tctccaggac acgtctactc ttatcacgga tccgccccag
    11881 atgtacaata acatccactc ccgaggcgat ctggttgcga atagccgtca gcggaagatc
    11941 cattcccatc aggaccatat tttccagtct tgccagcata tccgtggcac tgttggcatg
    12001 cccggtggac atactcccat catggccggt gttcatgcat tgcagcatat cgatagcctc
    12061 cggtccccgg acctctccta caataatccg gtccggccgc atccgcagag acgaacggat
    12121 cagttcccgg attccgattt ctctgcatcc ttcggtattg ccgttacgag tctctaaacg
    12181 aaccagattc ggaagaccct gcagctgtag ttccgcactg tcttctatgg tgatcacccg
    12241 ctcttccgcc gaaatatagc cggacagaac atttaggaat gttgtcttgc cgcttcccgt
    12301 tcctccactg acaaaaatgt tgtagcccgc cttcaccagt gtctccagga actgggctgc
    12361 ctccagcgaa atggtttcca gaccgatcag cttctgcatg gtgacaggct tgtccgggaa
    12421 tctccggatc gtgacgatcg gaccattcag agcaatggga ttcatgacaa tatttactct
    12481 ggaaccatta ttcaaccggg catctacgat gggtgacgct tcattgacca cgcgattaca
    12541 ccccgccaca atctgctgaa tgacatcctg taatttttcc acggaatcaa aggctcgatc
    12601 cagttcccgc agttgtccat cctgttctac aaaaatgtga tccatgccgt tgatcatgat
    12661 ctctgtaaca gagctgtcct ccacaaaaat ctgcagaata tccagtcttc tcagggaatc
    12721 aaacaattcc ttacgcagac gtctccgcaa ttctaccggg cataactcca gactttcctg
    12781 ctccatcagt acttcatcga tcgtatcctc cacttcttca tccgtataat ctcttccata
    12841 atccagtctt tcctgtacca tctgacgaag ttttcttctc tgctctaaat aatccatttg
    12901 tccttctcct attctactct tccaatgtct ctatttcttt tctcacataa tctgccagtt
    12961 cccctctggt atactgttcc atttccacgg gaagtcgctg aaagtatggc aaattcagct
    13021 ttctggtctt cccctttact tcctctttct cgcatagtga caatagctgt tcatattgat
    13081 ccagtttaca ctgtgacatc ttgtcctctc tggtcagcgt aaatactttg atacacatct
    13141 gcagaacatc caacagcccc tgtatgcttt cactcagatc cagaatgaca tattcgtatt
    13201 tccccagctc ctcgatcctc tggaacagac ttctccattc ttcccaggtg atccccaaca
    13261 ggttctgtcc gttccgcatg ggtggaatat aatccagatt gccttttctc tgaatgaccg
    13321 tctgcatgcg aagaggaaat tttccttcat ctcctgctaa aaaatagagc agatctgcca
    13381 gatcccggtc ctgtctctcc ggaagcaatt ctatgatccc cacataatgt tcgaaattca
    13441 gatacagcgt gggatgtttt tctgccatca gctgtgcgaa agacaatgcg aacgtacttt
    13501 gaaggcatct gtgaaccgga gagtacattc cgataaaacg ggtcttatat tccgcacata
    13561 acagcggcat ccgtatcccc gtcgtctcta catacaacgc caacagttcc ttccacacct
    13621 gtcccgcttc ctgatatttg ttaatattgg gaaactgatg aaagcggaga gttccgctct
    13681 catttaaaat tgcctgacat gtcggctgta atgtgttcaa tgcctcctca caggaggatt
    13741 ccgccaccac cagcattgtt acctccccgc ttttctctcc cgccaacagt tcttccgccc
    13801 tggtataagt atggatctgc cagggcaatt ccttttgcct cttcaggtat tctgaaaaaa
    13861 gccgcgcgta ttcttcctcc cggtcataca gaaccagaac cggttccttt tgttccatca
    13921 atacattcct cccatataca tcaatgcact gcagaacacc ggtcccgcca tgcaaatccc
    13981 tgctccggca ccttcccgtt ctccggcaaa ataaagtttc catcttccac accggaatac
    14041 gtcccataag tactctccca aatagtacaa acggtctttc atattgcgtt ccagatacat
    14101 tttgaaaagt gcaattactg ctgcaatcat gagcgcgaaa aacagaaaat aaagtacttt
    14161 attccaggga aaaaaaccgg cacagactcc caacatctta acatcaccgg ctcctatggc
    14221 tccgatccaa aaaacaaaat aaagtaatat cgttgtcagt aatgcacttg ccagatattc
    14281 caaaacgcct ttgtgcccgc cgctgatcaa ccgatacccc aggcctgcaa acatgcagca
    14341 tacgatcagc caattgggta tcctgtgcgt tttgtaatca aaacagcaag ctattgccaa
    14401 aagtatacat aataccccaa atatctcatc tccttctcct gatttcacat tattttcctg
    14461 cgtatagtcc tgtcgtcacc ttcctcccaa ctattcaaaa tcagtcttct ggtaatgcgg
    14521 gatgaatcta tcccaaaata gaaattctaa aagatcttat aagatgctct gaacaaccat
    14581 cgcgattggt tgtaagatga attataaatg ctcactttcc gtctggcaat acctttttcg
    14641 taaaaagata aactttagta cattttaaca aacagaattg tttttcatat tttcagtcat
    14701 cctgaaatac gctattttac acgctttccg tatgtcaaaa caccttttta taatatttca
    14761 taaatactta gcccgtacag catcacaatg cccggaattc caagaattcc cgttgtcaaa
    14821 atacttactg cattgattcc aaccactgaa gtaaatccaa ggaaagtcac acccatatta
    14881 ataaaataaa tggcaatggt tcccataatg ccccgcaatg caaaattgag gagccactct
    14941 gcctttttcc ccaaaaagct tatgagcagc aataatgccg ccactgctat cagccataat
    15001 aaattttccg gatgttccac tgttgttctc gcattcccga caattcttta tcctaatcta
    15061 ttcctctttc cgtgtaaaaa tgtcttccgt tttcctccat gtcaggtata tttttccttt
    15121 tttgttccta tactgttagc agaaaggacg gttaatgtta tgaaaatctt ttttagtcag
    15181 gcagatgcct ccccggaaat cattgaagag gatttctctc ccatagatct gaaagaatcc
    15241 ataagaaaaa cccgccaggc gttggaaatc gcctatgcgg gtttcgataa tgctttggaa
    15301 agtgatctca ttgacagtta tatttatgaa atcaatgcgt tacaaaaaag gtatcagcat
    15361 ctcacagagc tggccgctct gcagccggcg gatcaggaaa aaaatctata ccaacattcc
    15421 ccgattcgtg ccctggtcag ccatgttttc ggttaactgg ttcctgccgt aagtcagtcc
    15481 ggcatatact gtctgagaat tgttcatcac cgggccggaa gtatcctgat cctggatctg
    15541 cctgtacgca tcgcagagag gtgcgatcac tttgcgaatc tcttccaata ttgtgcggtc
    15601 tctgcggact tcacatacag caagtctgcg aaggcagaac aggtacagtc tggaaagctc
    15661 tcctgccggg ctgtattctc tgtgcagaga gttcaggagc tctttgatac atcccctggc
    15721 tctatgaaca gcttccgcca gtccggcatc atctgctgcc tgctctccgt ctgccagata
    15781 ttgtaaggtc atttcataca ggatcacgat caactgcgtg gaattcgcct gtgtaatccg
    15841 aagtgtaaat tgttgtttgc attcttttgt cattctgcca tgtcctctta ttcagaactt
    15901 tcctactgta acagctgcag tacctgagac ggtctctcgt tggcctgtgc cagcatggag
    15961 gttcctgcct gtgccaggat ctgataagtc gtataagaag tcatttcttc cgccatatct
    16021 acatccatga tacgggaata tgcggaagtc atattttcgc tggtaatatc cagactgttg
    16081 atcgtagatt ccagacggtt ctggaatgca cccagtctgc ctcgaacgga agatacatat
    16141 ttgatggcgc cgtctacgcg atcaatggca tcgtccgcac cttctgcagt nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cagcacaata ttgccgtccg catcctttgt
    16321 cagcggtata gactttatgg tatattcttt taccggcacg tcggtagaat agtagttcac
    16381 cttaacttcc tgcttgttgg tatatccctt cagatcgtac tccccattca gcagtttctg
    16441 tccgttgaac tctgttacgt cagaaattct ggtgatttcc tctttcagtt gctttacttc
    16501 atcctgtacg gtagctcggt catcatccgt cattataccg ttggcagcct tgacggagag
    16561 ctcattgatt cgctgcagca tctcactgat ctcggtcatg gcgccatctg caatctcgat
    16621 aatggatacg ccgtcgctgg cattctggtt tgccacggac aggccctcga tctgggcatt
    16681 catacgtttg gacattgcca gtcctgccgg attatccttt gcatgattga tctttaatcc
    16741 ggaagaaagt ctctctaatg atttggacag caggtcatca ttggtagata aggcattgtt
    16801 ggataacatt gccgatacat tgtaattaat tctcatgtgt tgttactcct tactttccgc
    16861 tgctgtctgc gacagcctgc aggaatcctt ttgccaggcg gtacagttgc gccgcatctg
    16921 cccggctctc tattgtttct gcattttgcg tctcaccggc agccatcctt gtcatatccc
    16981 gggagctgac aacagaaagc ttttccgctt tccagtctgc agaggtatct gtctgaatcc
    17041 attcaaggaa tatatcggag gtcttttcca gtttcgtaac ctcttccggg gtatttccta
    17101 cgagataacc ttcgatgtgt tctccgttga cctgtaaatg cgcttccaac tgttcgtctc
    17161 ccgcatctac ccggatatcc acagccgagg tctctccggc tccctgctgt agggtaagat
    17221 gcactcctgc cagctggtct cccagataca tcggcaggaa atactcctgc ctgttctgaa
    17281 gatttcctgc aaggcgcagc tgtttgtgta ccagctgtaa ctgtttcaca tccagatggc
    17341 tctctgcctg ttccagactg atcgtctctg tctcctgtaa agcatcctga agcatggtcc
    17401 ggtaatcatc tgcaaaattc ggctgatcca gctgttccca caatccggag gcttctgttc
    17461 cggcaggctc ccctccggtt gccgccacag gtatttcttt ctctttgttg tatttcttac
    17521 gatagcgttg caggtcgctg aacagttccg ccggatcctc cagcagagcc tgagctgcca
    17581 gcaggttaga agtactggta tttacctctc ccctttccaa catttgtgct gctcccggtt
    17641 cggtctctac cgcttcccgc atctgctgca gacattcctg ataatatgcg tctctgctct
    17701 cagcgtcttc tgacacatct gtcaatattt cttttgccat ccctacagaa atctgctctg
    17761 aaatattgtt ctccgataaa cgtacttcct gtgtcagtcc ggtatcatcc gacactttcc
    17821 agtccatatg ggaggttctt ctgctcctgg aagctgccag gagtgtggaa aattgcaatt
    17881 ctgcgccggt atttaccacc gcgcctactg cggcaccatc ttctctctca atctggtgta
    17941 ccatacgata gataccgata taagctttcc gctcattctc cgtgatatct ttcttttgtt
    18001 ccagctgata caggaaacgg ctgtagtcct ccgcctcttc ctcataactc tgaggatttt
    18061 cggcaaaata ggactccagt tccccaaaag tcttctccaa cggatttacg ccgtcccgga
    18121 tcatctgcaa tacattcttc ggtgtcagtt tctcaatcac tgcagtgacc tgtttgtctg
    18181 cttctttgac tctttcgata ttttctgcag taatctccat ccggttatac gcgaggatgc
    18241 ggaccgcccg ctggttctcc ggtgttttat ccaatgacat atctgtcaga atgtcatcta
    18301 cattggaaaa tgccttgcgg atgctgtccc ccaggtctct tcgaggagct gtcattaaag
    18361 tctcatagcg ttcgttcgct tctgtataag cggtctgcag cgccttgccc tcgccgtgga
    18421 attctgccac ggtatcctgc gaagctttct gttccaggct atagggtccc aacacgctga
    18481 ccggcagtcc cggcagttcg tttgtcacct gtacggtctg tgtataggtc tcatattttg
    18541 ccacggcatc cgcatcgttg gggaaatact tatccgcaac ttctgcctcc gctctacgca
    18601 gtgcttcgat cagctgctcc ataggggccg tatcaatgga aaaatcactc tgtaagagtt
    18661 tcacattcac ttccgctgtc atgcgcagcc ggatctcttc cagttgtttt ctggccgcca
    18721 gattctccgc cgtaacatcc cattttgcat ccgagaacca gtcctgtacc atttctgtgg
    18781 ctttctcgta tatattttcc tgccgtgcca gattcgcatg aacaggggat ttcccctccg
    18841 agagtgccga tgccgctgcc tgtgcaaaag catcctcact gacgggatag tcaatcgtat
    18901 ccagttctgc cagctgctgc aaggtatccg ttgtcagtgg caaacctgct cccaccagcc
    18961 actgcgcttt ctggcgattc tcatcattga ccggcaggcc ggcatccgtg atcaccttat
    19021 ccatctggtc ctgcaattcc tgcggtacgg tattctgctg tactttcgca ccgctgttct
    19081 ctgccacata caagttccag atctccggct ccaatccatt atctacaaga tacccgacct
    19141 cgccttcctg gggctgctgc agagaagatg ccagctccca ggcggttttt aattcatcta
    19201 cattttcagg agtcagaggt atatccgttg cccggaactg ttcttcaata ctttgtgcca
    19261 gtgtatcgct gcccacagct gccgccaggg tgtccagatc tatgtcgtcc gtgtatccgg
    19321 caatgttctg tccggaacgc accagttctg ctttgatcct gtccacgatg gtaactgcgg
    19381 tatcctgatc cagttctctg gggtcaaatc cgtcctcaca agccttcgca taatcctcct
    19441 gggacatagt atgtgacagt agtgtcatgt aatcctgctg taatgccaca ttggtgttcc
    19501 cggcatcctg ctgcagttca atcagggatt ttcccttatt attctcttca tttcccacag
    19561 gcattgggat ctgcccactc tcgtatacgg ctccaaaagt atttgcattc acactttttt
    19621 tattgcgctg tattttatct ctgccccagg tatttttctc cgtctggata ctttctgttc
    19681 ttttttctgt atcagttacc gtctgattct gaaatgttat cttcacgctt gtcgtccttc
    19741 ctggtaataa ctcttgaaat aactattcag agactcattc gcaaaatcgc tctttcagag
    19801 ctttcctttt gcccattgag gctatatcgc cctataaata tcactaaatc ctcttacgaa
    19861 tacaagcatt caaagaggcc ttagtgatat ttacatgtct ctgaatagtt aagaaaaaag
    19921 atgagtgcac cttttggtca cactcatctt ttatttcggt cgtttcttat aatttcttaa
    19981 ccggacttag aacagaaata cagcagctcc ataagggggc agatcaaagc tgatagaata
    20041 atccttgtaa tggtaaggct ttttctctgc catgatctct gcgggaattt cattgcccca
    20101 gccgccgaaa cgctcctcat cgctgttgag cagcagctta tatttcttct ttttcggcac
    20161 gggtacggta taattctccc atttcatcgg agtcatattc aatacaaaca gcagattgtt
    20221 cttgctgctg gaacattttc tcacaaagca ataggtgctg tgttctgcat catccgcatt
    20281 catccactcg aaaccgtccc aggtattgtc gatctcatac attgcaggat acttgcggta
    20341 cagttccaac agttccttta cataggtatg tacaccctga ttcagcttct cgcctaacag
    20401 ataccagtcc agttcgcgtt cttcactcca ctcacgctcc tgtccgaaat cctgtcccat
    20461 gaacaacagc tttttaccgg aatgtccgaa catataggta tagcccacac gcagattcgc
    20521 gtatttatcc gcagtataac ctggcatctt atttaccatg gaacacttca gatgtaccac
    20581 ttcgtcatga gacaggggca gaatgtagtt ctcactgtca ttgtagctca tggcaaaggt
    20641 catagcgtaa tgattgccct tacgatacaa aggatccagc ttcatgtatt cacagaaatc
    20701 atgcatccag cccatattcc atttaaagga gaagcctaat ccctcgcctc cgatatcgct
    20761 ggtcacattc ggccacgccg tggattcctc ggcaattgtc acaaatccgg gatacgttcc
    20821 acggatgaca gaattcagat ggtggaagaa ctcaatggcc tccagattct tgttgccacc
    20881 gaatttattc ggcagccact caccttcttt tttaccataa tccagatata gcatggatgc
    20941 caccgcatct acacggatgc cgtccacatg gaactctctg agccagaaca aggcattggc
    21001 taccaggaag ttctttactt catttttgcc gtaattgaag attttggttc cccattcggg
    21061 atgctctccc agacggggat cgggatgttc aaaaatgcac tgaccgtcaa attcagccaa
    21121 gccatgggca tcagttgcaa aatgcgcagg tacccagtcc agaatcacgc cgactccggc
    21181 cttgtgcagt tcattgatca ggtacataaa atccttcggt gttccgtaac gggaagtcgg
    21241 tgcatagtaa ccggtcacct gatatcccca ggaaccgtca aacggatgct ctgcaatacc
    21301 catcagttct acatgggtat acttcatctc tttcagatat tctatgatcc ggtccgcaaa
    21361 ctcccggtaa ttgtagaatc cttcggcggt accgttgtta ggatgcttca taaaagaacc
    21421 gatatggcat tcgtagatcg ccataggctc tttattcata tcctttttgt cccgggcttc
    21481 catccacttg gaatctctcc agtcaaagcc tgtaatatcg gtaacgatag atgcagtgcc
    21541 cggccggtat tccgcataat tggcaaaagg atcggctttg tagagctttc tgccatccct
    21601 tgcatagatc aggaacttat acatcgttcc ctcttccacg ccgggaatga acagccccca
    21661 gataccgcct tctcccagct tctgcatggg atgggattcc tcattccatt cattgaatgt
    21721 gccaatgaca ttcacctgtg ctgcatttgg tgcccatact ccgaaataga ctccctgcac
    21781 gccgtcttcc accgacagat gtgcacccag ttttttgtaa atatcataat gggtcccctg
    21841 ggcaaaaaga tactgatctg cttcggaaat aaataccttc tccttcgtca tggccgcgcc
    21901 tcccgctata aaatttaact gttcagaata agttatacaa aatctttctc ggtgaggatg
    21961 atcatatcct catcgtcata tctcttggcg aacagtacat cgaagaaatg cttcaatgtc
    22021 tttttatttg catgagcgat cgcttcatcc acgatctgct ttacatccac taccgtaacc
    22081 acatgatcta tcgtctgcat atcttcgatt ctggtatgta acgccagctc tccgagttcg
    22141 tcaatagagt attccatctc ccgtgcatac tgtttgccga atgccaccag cgtgttattg
    22201 ctgagggctt ccacatccat tcgtgcggta aagcactctc tcaatttttc atgtttctcg
    22261 aggaatttat caatatccct cttcgtatct tccagaataa ttacgattcc ctgactctcc
    22321 tgctgcaatg ccttgtgaag tacatttgcc gtagcatcgt tcatttcgga tgccttgcaa
    22381 atgatcagcg cgccgttttt cagcttggat aaggtatctg cagtatcctt tttattcaat
    22441 gcatgtccag agatctttgc cacttttccg gagaaattgc tgtcggttgc ctgaatttcg
    22501 cgaaccatgt tcttcgccag agataaggta tccataccct cttctccagt gatgatcacg
    22561 ttgccggtgt aggctgccag actgatatta tcaatggcct tgactaactg ctctctggca
    22621 gatctgctct ggataaaggg tgcgtacagt tctctctctt ctctggtaag cgcacgaacc
    22681 tttgctttgt ctcgctctac tgcggggcgc tccggctgtt ccggtgcttc cccggcttcc
    22741 ggcgtttcct cagccgtcag ttctatctct tcgctggctg tttcctcatc taccgattct
    22801 actgcttccg ccgtgggctc ctctacaggc tcgtcatcca ttgcttcttc cggttctgcc
    22861 tgttcctcgt ctacgatttc ttcctgctca tagatttctg cttccggttc ctcaaattct
    22921 tctgcttccg gctcatatgc aggttcttcc gcgagttctt ctacagtatc ctcttccgga
    22981 agctcttcta caggctcttg ttccgcttcc tctgcaggcg cagcctccgt cagttcttcc
    23041 acgggttcct ctgtggcagg ctcttccgtg atctcttcca gttcttctac ctcatcggta
    23101 tctgttgtat cttctgccac ggtatctgcc gtgtccgcag ctttttcttt ttccagtttt
    23161 tccagcaatc cgtctcttac cgcctgctca aactccgtga acatggctcc ggtctgctgt
    23221 aatacatgtt ggcgaacctc ttcttctctc ttctccaggt tctccttctt cttgcgttcc
    23281 cactctgcca gaatatcctc gatattcatc tggccggtga tctgtttctc aatgctctct
    23341 gcctccggca ttaccaggct gatctgaccg tcggattcct gtgccaacac atcggccagt
    23401 tccttgggcg gctctacggg agctgccggg gttaccggtg ctacaggagc tgccggcgcc
    23461 gatgccgtaa cttcatgaat ctcctgcgtt tgggaagctt ccggtacttt ctgtgtttcc
    23521 ggttccttca cgatttcctg tacttccggt gtcttctgta cttcggatag ctccggtact
    23581 acatcggttc ttcttgcaac aacaggctct tccggcagaa tctcttccgt ctctacctgg
    23641 ggcacctgtg acagatcgcc gatttctccg gtctccccaa agaatacttc cgagctctcc
    23701 atctgctccg tctccggctc cagatcgtct tcactgacat ctgcgatctc cggatagtcc
    23761 agagactccg tatcggaatc cccgggatcg atcatgggtg ccacgataga acgggtaatg
    23821 gaatccgaag tctccttcgt atggtcctca ctcagtacct cccgcaggcc tgctgccagt
    23881 tctgcctgta gattgatcgt attatattgt cccacatcca tggtcttcac atgaatgtcg
    23941 aaatcatctt ccgccggctg cgcgggtgcc tgctggggct gtaccggctg caccgtttca
    24001 tagactctgg tgtcaccggt cacctgctcg taggtttcct cctgtacata accctgattn
    24061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc atagccctga ttcggatcat
    24181 aagtttcgtt ctgtccataa ccgggatcct gcgcataatt ctgagaatac tgctgcgtac
    24241 cgggcgcgtc aaaacgatga tcgtattttt cctgctgttc cggtgttagc ggctgatgga
    24301 gcattttcag ctccattgcc ttgataacgt atttgccctc gccaaaccaa aggatcagct
    24361 catcacattc ctctacacat ctggcagcaa gtcctactct atgatataga taagccaact
    24421 catatgccca tttttctctg taatctctct ttttcagttc ttccagaaca gcaatgcgct
    24481 cttccagact gacatcctgc gcctcatata atttgtactg caaaatatat ctgccgggat
    24541 ccttgggcgc tacctgcaca aactctttgt aatactcgat agcctgtaca tactcttcca
    24601 ttttaatgga aagttcgcac agagaatagc agatcgtcct tcctcccgga cggcgttcat
    24661 atgccagaag cagcatatct ctggcatcct cgtatcttcg attgatcttg taaaggtcac
    24721 taatggtgca cagcatcata acacttttga ctctgcgcca gtcaatggta tccgcaatct
    24781 ccgcagcctg cgcatactcc ccacggctga tcaggtcttt gatttcttcc gctcttaatt
    24841 tatattcact cttatccaag gtatttcgct cccttaacct tttcttatcc taagcttctc
    24901 attcccccgg gtatactacc ccactttttt cttattcagt gtaccatagt acccattgat
    24961 atgtcaaaat caaactgggc aaaatgtatg gaaatctccc cttcggaata ctacccacga
    25021 aagtgggatg acttcttggg cttttgacgt tttttcgcct gtcggattgc ttctttgcgt
    25081 ctgttacgaa ggtcctgatc caccgaacga taccgggatc tggaacgatt tcttctgcgg
    25141 ttaaatctcc aggtcaggcg ggtactgcgg tgggcaaaat gataattctt gaaaaacgta
    25201 cgtaataaca gtgcaaggaa gatcaatacc gcaattccca cgatacagaa aaatactttt
    25261 accacattaa tgaaaataac cgcaggaccg ccggaagtgc tgttttcctc ctccggggat
    25321 gtctcctgtg cagccgttgg aattccacca ttgtttccgg tgttatctcc tgctgtttcg
    25381 gttgtctctg tattgtctgc ctctgtggtc gttgtgcctg actgcgtttc atcggttgca
    25441 ccgctttcct ctgcagactc cgtggtctct gccgtatttt cccggaagac actactgccc
    25501 ttttccgctg ccgtaaagtc cagggatgcc gttcccagta ccacatcatt gtaagtataa
    25561 gtgatcacag ccacctgtcc tggttctgta ttatcatagg aaatactgga caccgcatcc
    25621 tgaaaggtga tggtgttcgg caaagtgatg ctgtccgtct ggttcagttc cagaatcggt
    25681 ttggagttgc cgaaaatatc attcccactg taaaaagatc ctgcattctc gatattatac
    25741 ttggtctctg tctgagaaat gttcactctc tgaaaattgc tgaaaccata atcaaacaga
    25801 gtgattgtat cttcatagta attcggattt tcgtctttca tgaccacaca gatcagcttc
    25861 atgccgtctt tttccgcaca ggacaccagt gtactgcggg caacatcggt gtaaccggtc
    25921 ttacagccta ccagatacgg atatgcatat ttaccgccgg ggatcagttc catcttgttg
    25981 acttccataa tatcatccgg ctggcgttcc gacggcaaaa tgtgcagcat atgtgtcatt
    26041 gtcattttac acagcgcttc gttggcaaaa aaagctcttc cgatcatcgc caggtcatag
    26101 gcactggtgt aatgatcctc attgggaagt ccgttgggat tgacgaaatt ggaatcaaca
    26161 caccccagtt ccttggctct ttcattcatg atcggtgcaa agtcctgcca ggtcgttccg
    26221 gaaatatgct ctgctaccgc gaaagaaact tcattggcgg agcggatcag aatggcattc
    26281 agacattcct ccatggtcag ctgctcgccg gggtcaatag cgatatggtt gcttccggga
    26341 tcaatatcgt gtaccgcatc ataggagaaa tccacgatct catccatgct gcagcgctcg
    26401 gatgcgatca atgttgtcag aattttggtg gtactggcag ggtattcctg ctgatggata
    26461 tttttggcat aaaggatcgc tccggtatcc gcatccatca aaatggcgga ttctgcgctg
    26521 actaccggtc ccgtaggcca gtttgccact tcattggact gaatggccag tgcctgattg
    26581 tgggcgatgt tctcttcttt gatcgttgca gcagagacac taagcccctg agatgcaaaa
    26641 aggcagagag cgctgattcc catcactgcc ctggttaaaa aacttctata tgaaaaatgc
    26701 ctgaaaaact gcatgtataa atccttcctg atatcttcaa aataaaatgg tgtatgataa
    26761 tgcgcattct ttatcataca ccattttacc taaaaaccaa agtcttatct ctatttttta
    26821 attcttttta agaccttctg cgaacgctgt cattctctat acggaatacc gtaaatgttc
    26881 ctgtccgtcg aagtctttcc acacaaaatt tttgccttcc cagatcatgt atccgtgcca
    26941 gctttcttcc ttgggaatcg acacggaccc cggattcatg cagatatact gctctgtctc
    27001 atgaacaacg catttcggca catgagtatg accgtgcagt aaaatatctc cggaatgcaa
    27061 tggcggcagt ttctgctcat tatagatatg tccatgggtg gcatagattg cctgcgtccc
    27121 ctccgcaagt acacaataat cactcagcac cggaaattcc agcaccatct gatccacctc
    27181 cgtatcgcaa ttaccgcgta cacagtagat atcatttttc cgctcgttca ataaagcgat
    27241 cacttccttg ggcgcataat ctctgggcag atcatttctg gggccgtggt ataaaatatc
    27301 ccccagtaaa agcaacctgt cggccccttc ctccgtatat gcttccaaca gttttcgaca
    27361 gtaatatgcc gatccgtgaa tatccgatgc aatcatccat ttcataaatt ttctcctcct
    27421 actgccagta taatcccccg gcctgtgact tgacaagctt tctcagataa agtacattga
    27481 caatacagat tactataaga aaggctgaga ttaccatgag gttacgaaat attaccggtt
    27541 cccgggaagt tattgcggaa agtccctatg tcgtaccgga agataccctg gaagcatgcc
    27601 ccggaacctg gcatgagatt ttcggaaacg atcatccgat ccatattgag atcggtatgg
    27661 gaaaaggacg tttcctacat acccttgcaa agcagaatcc gcagattaat tatgtgggaa
    27721 ttgaaaaata ttccagcgta ttgctccgcg cgatccagaa aatggaagag gacgagcttt
    27781 ccaacctgaa attcatccgt atggatgcag aagacatcga taaggtattc ggaaaaggtg
    27841 aagtggaccg catttatctg aatttctccg atccctggcc caaggaccgt catgcgaaga
    27901 gaagacttcc ttcgagacag tttctggccc gctatgatgt gatcctgaaa aaagagggta
    27961 cgctggaatt caagacagac aaccgcccgt tgtttgattt tgcaatagag gaactggagc
    28021 ccgccggatg gaaagcgcag gtgatcacct acgatctgca taacgatgcc gcgctgatgg
    28081 aggggaacgt catgaccgaa tacgaggaaa agttttcctc cgttggcaat cccatctgca
    28141 aatatattat ttacagataa ggccccgaat atagaatctt atttttgaga tactatcaac
    28201 aacagataac agaaaggaag gatacttatg gaatatacgt ttaccgttgc taattttgag
    28261 gaagaagttc tgaactcccc tctgcccgtt ctggtagatt tctacgcaga ctggtgcaat
    28321 ccctgcaaga tgatggctcc taccgtggca aagctggcag aagcttacga gggaaaagta
    28381 aaggtcggca agatcaatgt ggatgagaac atgaccatcg cccagaagta ccgcgtggca
    28441 agcattccga attttgtgat cttcagagac ggacaacccg tggccaactt cgtcggcgcc
    28501 atgtccgaag cagacttccg tgagaagctg gagcaggtga ttctgtaaaa atcagttttt
    28561 taaacccagg tacggtgagc ggatgcctct gccgtatccg ggtttttgat tttttacaga
    28621 tattttcccg taatgctgtc ggcatctgcc ttgatctgct ccggagttcc cacggcaacg
    28681 actctgccgc catcttctcc gccaccgggt cccatatcaa tgatgtaatc ggcattgcgg
    28741 atcacatcca gatcatgttc aatgacaagt accgttgccc cattgtctac cagcgtctga
    28801 aatacctgca acagggtctg tacatctaac ggatgcagtc cgatcgtcgg ttcgtcgaat
    28861 acaaatacgg aatctgattg tcctctgccc agttcactgg ccagcttcag ccgctgggcc
    28921 tctccaccgg agaggcttgg tgtctcttcg cctaatgtta aatatcccag tcccagattt
    28981 ttcaggactt ccagccgctg atgcacgact ttccaatcct ctgtcgcctg caatgccgtc
    29041 gttacatcca tctccatcat ctgcggcaga gaataacgtt tcccccgttt gttctcatag
    29101 cagatgtccc atgcttctct ggcatagcgg gaaccaccgc actccgggca gggcacatcc
    29161 acatccggca gaaactgcac gtccagactg atctgtccgg tgccgtcgca gaccgggcat
    29221 cttaattttc cggtattata agaaaaatct cccgccttgt atttcctgtt ttttgcatcc
    29281 gcagtcttgg catagatctt ccgcagttca tcatgcacat tggcataggt agccaccgtg
    29341 gaccgcacat tgatgccgat gggggaagca tcgatcagct tcacatgggc gatgccctct
    29401 gcggtaatgc tttttacatg tgctgggaaa tgttcgccgt ttaattttgc ttccagcccg
    29461 gggatcaggc tttccagtac catggtggtc ttcccggatc ctgagacacc ggtgactacc
    29521 gtgagccgtc ctttgggaat gtcgatctcc agagatttta ccgtgtgaat cgcatccgta
    29581 gacagatgaa gctttcccgg tgcaaatatg tcctcccggg cgatgggttt gcgaattcgc
    29641 atattcgctg ttccggccag aaacggcccg atcatggact ctttctgtgc gatcacctgt
    29701 ggaataccgc cctgtgtgat cacataaccg cctccggcac cggcttccgg tcccatctcg
    29761 atcagccagt ccgcttcgga taatatctgg gtatcatgat ctaccaaaat gatggaattg
    29821 ccatcagcaa ttagatcatg catcacggca ttcagtccca cgatattcga aggatgcaga
    29881 ccgatggagg gctcgtccaa cacatagagt accccggtgg tacggtttcg caccgcccgg
    29941 gccagctgca tccgctgtct ttccccggtg gacagggagg ctgccgcacg gtccagagac
    30001 aaataagaaa gtcccagatc catcagcctt ttcgccacag tctggaaaga ttcacagatg
    30061 gattccgcca tggggcgcat ctcttccggc agagaatccg gcactcccgc tacccagtcc
    30121 accaattctt tcaggggcat ctggcaggcc tgtgccagcc ctatccccct aagtctcggt
    30181 actctggcag cttccgaaag tctgctgccg ccgcattctg ggcagatatc ctcctttaag
    30241 aatttttcca cacgtttcat gcccttttca tcttttacct ttgccagagc attctctacc
    30301 gtgtataccg cattaaaata cgtaaagtcc agttctcccg cctgattggt cttcttcgcc
    30361 ttatagaaaa tatgtttctt ctccgcgggt ccgtgataaa cgatctcttt ttcccggtct
    30421 gtcagttccc ggaagggtac atctgtccgc actcccattt cccggcagac atccgtcatc
    30481 aatgaccaca tgaggctgtt ccagggtgct accgctccct catcaatgct tagggaatca
    30541 tccggcacca gagaatccag atccacggtc cgcacacttc ccgtacc
//
LOCUS       DAWEJW010000021        30409 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_636_length_30409_cov_64.205706, whole genome shotgun sequence.
ACCESSION   DAWEJW010000021 DAWEJW010000000
VERSION     DAWEJW010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 30409)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 30409)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..30409
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_636_length_30409_cov_64.205706"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3752..3851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8723 a         5999 c         7966 g         7621 t
ORIGIN      
        1 gataccttta atcccgtatt ggagaatctg actgtgaatt ttaatggaac tgcatgggca
       61 gagggaacaa actatacgta tgatacgact accggattat ttaccggaac tgcaggaggt
      121 attaccgtac ccgcagcgac ttacacccag gatcccgtta cgggagcgtg ggggatcaac
      181 cccggcgtca gcacgctggt gatctccgga actgtataag ggctgccgcg ctcctaaggc
      241 tggcgccatg caacccattc gcagtcactg cgtgacttgc tcatggacgg ctgtcgccgt
      301 ataaatctaa aataaaaagt cactggaagt acataaatgt cctcccagtg actttttatt
      361 ttatccttgc atggcttgta gcttagccaa agtatctctt taacagaccc tcaaatgcct
      421 taccatgtct tgcctcgtca cgagccattt cgtgtacggt gtcatggata gcatccaggt
      481 tcagagcctt ggctctctta gccagatcaa acttacctgc ggtagcaccg ttctcggcat
      541 ctactctcat ctccagattc ttcttggtgg aatcggttac gacttcacct aacagctcag
      601 cgaattttgc agcatgctca gcttcttcgt aagcagcctt ctcccagtac agaccgatct
      661 cgggataacc ttctctgtga gcaactcttg ccattgccag atacatacca acctcagagc
      721 attcaccctg gaagttcgca cgcagatctg caatgatatc ctcgggagca cctttcgcta
      781 cacctactac atgctcagca gcccaggtct tgccttcctc ctgcttggtg aacttggaag
      841 cgggagcgtg acagatgggg cactctgcgg gagccgcatc accttcataa acataaccac
      901 atacctgaca tacatacttt gccataattt tttctccttt tctctaatga aatgttttgt
      961 tttagttttc tctttttgcg tgtgctttat aagcacaaat atattatgga acgaagccag
     1021 ttttcattca gaaaactagg actccgtagc aaccctggtg caggcgggac aagtgccata
     1081 gaaataagtg acatgtcccc ggatccggcc gtcaaatctc tctccggctg tctccatgat
     1141 ggaatcaata ctatccatgt ccagatcaat gacgcttccg cattccgtac acacgaaatg
     1201 ataatgttcg gaagtatcag catcaaaatg gtcaacaccg tcaccgaccc ggagtcgtaa
     1261 aatctctccc atgtccgaca acagagtcag attacgatag acggtaccga gacttaagtt
     1321 cggatcctgc tgcttcagat tagaatagat cacatcagcg gtagggtgat ccttgcgtcc
     1381 catgagaaag gatttgatca tttcccgttg tctgcttcgt ttcagtgcca tgataagccc
     1441 ctttctcgat aatagtaatg attactgttt cttataagct tattatacat gatgcgagaa
     1501 taatgtcaac agaaaaatga aaaaattttg tttatatttt tttaatgata ttcctgcttc
     1561 ctttatacac agaaggtggg aatcatgcta taatgagcga aagtaaagta taaaatataa
     1621 attattcacg tgcaggttgc tgaaaggcaa gagtaatatg aaatttaaaa caagacttcg
     1681 tgtaacattt ataagtatta tattacttcc gttattgttg acgatcatgg cttttgtaat
     1741 gattgccatt tacctgatga attactctca gggtctcagc ctgacggata ttgattattc
     1801 catgatgtcg gagaatttca gggaattcac caacaccacg gatcaggcgt attatgtgct
     1861 gctggaccag gtgaaggaag acagcagcag gctggaagat aaagaatatc tggatcatat
     1921 caatgaggag gtatcgcgga agtctactta cattattgta agaaaaggtg ataagctgta
     1981 ttatgccggc aatgaagaag ccgcacagca gatttttgaa aaactgcctg cctatggtga
     2041 cgagaatctg tcggatgaca gcggatattt ctacaatgaa ctggagaagt atgtaaaaca
     2101 gattgatttc accttccggg atggaacacc gggcagcgta tttatcgtca cgaaggtcaa
     2161 ttcgctgatc tcgagacatc tgctgatcga tatgttcgtg gcgatcattg tgatcctgat
     2221 cttcaccagc ctgatgctga cccagtggat ccacaagggc gtatttgaac ctatcaatga
     2281 gctgaatgtg gcaatgcgca agatcaagga aggcaacttt gattatgttc tggagacgga
     2341 tgccaagggg gagatcggtg atctctaccg caattacgag gatatgcgtc tgcggctgaa
     2401 ggaatccaca gaagagaaca ttcagcatga gaagcagaac aaagagctgg tcagcaatat
     2461 ttcccatgat ctgaagaccc ctatcacagc cattaagggt tatgtggaag gaatcatgga
     2521 cggcgtggca gacacaccgg agaagatgga caaatacatc aagaccattt acaataaagc
     2581 cattgatatg gatcgtctga tcaacgagct gaccacttat tccggaattg ataataaccg
     2641 gatcccgtac aatttccacc ggatcaacgt ggcggattat ttcggtgact gtgtggaaga
     2701 agtcggactg gatctggagc agcggggtat caagctgaac tattcgaatc tggtatctcc
     2761 tgacacagtt gtcatcgcgg atccggagca gatgaagaag gtcatcaata atattatcag
     2821 taacagcgta aaatatatgg acaagcctga cgggcatatt gatatccgta ttcttgacga
     2881 agtggattcc atccgggtag agatcgagga caacggcaag ggaatcgcac agaaggatct
     2941 gcagaagatc ttcgagaggt tctaccgcac ggatgcttcg cgcaattccg cccagggcgg
     3001 cagtggcatc ggattatcca ttgttaaaaa aattgttgag gatcatggcg gttatgtatg
     3061 ggcgacagcc aaagaagggg aaggaacctg tatgcatttt gtattgagaa aatatattga
     3121 gctgcagccg ggtggaacca agtaatgaat aagggaatga aacagaaatg gcatgataat
     3181 cgataataca aggacattct gtaaatggag gataaataat gagtaagatt ttaattattg
     3241 aagatgaagt agcaattgca gatcttgaga aggattatct ggagctcagt gatttcaagg
     3301 tcgatatctg caataccggc gatgagggac tgaagaccgc actggcaggc gattatgatc
     3361 tgatcattct ggacctgatg ctgcccggca tggacggctt cgaagtatgt aagaagatcc
     3421 gcgaggagaa aaatattccc attctcatgg tatctgcgaa gaaggacgac attgataaga
     3481 tccgcggctt agggctgggt gcggatgact atatgaccaa acctttcagc cccagtgaac
     3541 tggtagcccg tgtgaaggcg cacatggccc gttatgagag actggtggga actaaccaga
     3601 agcagcagaa tgatatcgtg gagatccgtg gtatccgtat tgataagact gcaagacgtg
     3661 tctatgtgga cggtgtggag aagacgttta cgaccaagga attcgatctg ctgacgttcc
     3721 tggcggagca tccgaaccat gtatttacca annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn naggaattcg atctgctgac gttcctggcg gagcatccga accatgtatt
     3901 taccaaagaa gaattgttcc gtgagatctg ggatatggat tctatcggtg atattgccac
     3961 cgtcacagtg catatcaaga agatccgtga gaagatcgaa aaagatactg caaagcctca
     4021 gtatatcgag accatctggg gtgtgggcta ccgatttaaa gtataaaaga agaaaaataa
     4081 gatcagaaga gaactcctat gatggataca gcaacaaact gtctccaata ggagtttttt
     4141 atctaaaagt gtgttataat catagcattg cggattgcac ccgtgaaatt tgattagcga
     4201 ttccactgat gagataaagc atcggcggaa tggagtgtgt tatgaacagt aaaaaaataa
     4261 tacagttgtc tacgacacag aagatcatgc ttagctttct gtgtgccata ttagtgggta
     4321 gcttcctgct ggcattaccc atcagttcag ctaccggaga agcggttcct tatatagatg
     4381 ctttatttac ggctaccaca tccatatgtg tgaccggtct ggtgacggtt cccacatatt
     4441 ccacctggag cgtctgggga cagatcgtga tcctgtttct gatccagttg ggaggtctgg
     4501 gagtcattac ggtcatgtac ggtgtgatga tgggactgca cagacgactg ggactgggta
     4561 accggtggat gctgcaggat gtatttaatc tgaataatat ctccggaatc gtgaaattcc
     4621 tccgcaaggt tctcataggt actctggtgg tggagggatg cggagctctt ttgtacatga
     4681 cagtatttgt gccgggatac ggactcagag gcatatggat ttcgattttt aatgccgtat
     4741 ctgccttctg caacgccgga atggatatta tggcggaaga cagtctgtgc ggctatgtat
     4801 ttcagcccat ggtcaatctg gtcacgatgc tgttgatcat attaggaggc ttaggctata
     4861 ttgtctggtg ggatgtactg cgggttttga aaaatatccg gagccagaag ctgaaatgtt
     4921 tccgactgct gacgcttcac agtaaaatag cactcacggt gacaggaata cttattgtag
     4981 tgggagcggc ggtattttat atttttgaat acaataatcc tctgacgatg caggattaca
     5041 cggtgccgca acggatctgg gcatccctgt tccaggcagt aactacgcga accgcaggtt
     5101 ttgccacaat tccacaagag gatctgacca atacctctgc gatcatttcg gtgcttttga
     5161 tgctgatcgg cggctctccg gtgggaaccg caggaggaat gaagacagtt acgatcgcag
     5221 tgctgctggt atctatgttt gccactatag ggaataagga ggatgcagag ctttttggga
     5281 gaaatattcc gaagcaggca gtgaataaat cggtagcagt tgtaggtatg ttttttatca
     5341 ttgcgtcatt gtccacgatt cttttatctg cagtgacaga tgcggatgtg atcgatattg
     5401 tgtatgaaac agtgagtgct acagctactg tgggattaag caggaacctg acttccatgc
     5461 tgaatctttg gggaaaactg attattatcg tgaccatgta tctgggaaga gtcggcccca
     5521 tatcgctggt agtggcattc agcacgcaga aaaagaacaa aaatattgtg aagaacccga
     5581 cagaagagat cagcgtcgga taaagcagat cgaagcaaca ggaaggaaga gactatggga
     5641 atcaatcaga cctatgcagt atttggactt ggaagatatg gaagaggtgt ggcgaaggag
     5701 ttggcagaga acggcgcaga ggtggtagcg gtggacaaca gagaggcagt tgtcaatgcg
     5761 gcaatttcag agattccata ctgtaaatgt gcggatgtta cagatccgga agcgttgaga
     5821 cagatcggta ttgcgaatat tgatgtggtc atcgtgacca tggcaaatca tctggaagcc
     5881 agtgttatgg caaccatgct gtgtaaagaa ctgggcgtaa agaccgttat caccaaatgc
     5941 gccactgaga taaactgcaa gatccttacg aaagtaggag cagaccgtgt agtattcccc
     6001 gagagtgagt cagggatcca tctggccaaa aatctgttaa gctccggctt catggacatt
     6061 attgaattgt cccaggatgt gtctatggtg gaaatggaag tgaaacagca gtggatcggg
     6121 aagaacctga tcgagctgaa tctgcggaaa aaatattcca ttaacgtggt ggcgatcatc
     6181 gaaggaaaaa atgtaaatac caatatcgat ccggaagtac ctctgacaca ggatatgcgg
     6241 ctgatcgtta ttgccaaccg ggcaaaattg aataaactga aatagaatag cttcgagcta
     6301 taaaaaagtg aaacatctga cggtgcactt gagcacaaga cagaatgttt cactcttttt
     6361 ttatacggtg gcagccgtct gaaaagttgt aaacgggtga tcagttatca gcccggaaag
     6421 tcttctcatt aaaggcactc tgctgcttgg caacgatagt ggtacatacg gcatcaccgg
     6481 tgatattgac agcggtacgc agcatatcca ggatacggtc gatacccatg atcagtgcaa
     6541 tgccttcggt aggcagtccc acggaggtca gtaccatgga cagggtgacc agaccgacac
     6601 tggggacacc ggcagtaccg atggaagcga tcgtcgcggt ggcaatgaca gtagcaatgg
     6661 cagccggggt cagaggaata ccataagcct gtgctatgaa aacaactgct acgccctgca
     6721 tgatggaagt accgtccatg ttaatggtgg cacccagagg aatcgtaaag gacgaaatct
     6781 gtttggagac accgattttt ttatacagag tgtcgataga cagagggatc gtcgcattac
     6841 tggtggaagt ggagaaggca aaggtcataa ccgggaagaa ctttttaatg aacttaaaag
     6901 gattcaagcg ggtgaacaca aacagcatga cctgatatac cacgaaacac tgtactgcca
     6961 gcgccagaat gacacttccc atatatttca gcataggcac aaaagcgtcg aagccgatgc
     7021 ctgcgaaggt cttcgcaatc aggcagaaga caccgacagg ggcagctttc atgactgcaa
     7081 tcgtcatttc catcataatg tcattgaact gactgaaaaa gttcgccaca gtggagaccc
     7141 ggttgcccat ggcagccaga agaatgccaa caaataaagc aaagaagatg attggcagca
     7201 tatctccggc ggacagagag gtaaaaatat ttttcggaat aatattcaac aacgtctccg
     7261 caaaatctac ctgggtggtt gcgacttcgg tggcatcctc cggcaacgca atatttaaac
     7321 cgacgccggg attgatcaga ctggcaatac ccagtgccac gcagatcgcc aaggcagtgg
     7381 tgcacagata gaacaggatc gttttcacac ccacagttcc caacgttttc gtatcaccga
     7441 tggccattgc gccgcagacc agggagcaga ataccagagg gaccaccagc atctgcatca
     7501 ggcggataaa accatttccc agtacataga gaataccgtt gatcagaatc gtatctctga
     7561 agtacccttc cggcatataa taatggatca gtacaccaca taccgcaccg gttaacaggg
     7621 cgataaagat tttggtggac agaccgagtt ttttcttttc ttttttgtgt ttttccacta
     7681 ttccagtcct ccggtttctt tcatgtagga agccagtgtc tctctccagc cactgggttc
     7741 ttcgatgccg gtgagacgca gcatcatatt atccagtacg gaatatttct ccggggtgcc
     7801 gtcttttact gtgacggtaa gccggtcctc ttttccggca agacgcagga tctctttcgc
     7861 aaattccagg cgtgtgcagg aaccgccact gcatacggcg tgatagatgc cgtaatgatc
     7921 gttgtcgatg aagaatttta ccacgcgtgc aagctctgcg gcggaggtag gtaccgcacg
     7981 gtcgttattt agcagttcca tagtagatac ctgggagtcc gcggcagctt cgagtacttc
     8041 attcaaaaag tctttgccaa tgccatagat ccaggaactg cggataatga catagcgggt
     8101 catcagctgg atgacgaatt tctctccggc atatttggat ttcgcaaaca gccccacggg
     8161 atttacatca tcgaactcat tatagggtgt gtcggatgcg ctggaaaata catcatccgt
     8221 agagatctgt atgagttttg cctgaatgct ctccgcagac tgcgccagat ttctcgcacc
     8281 tacggcgttt accttgtaag ccatatctgt ctccgactgc gctgcaccgg cggaaggatg
     8341 atatcctgca cagttgatga cgacatcggg acgattccgg tgcatgaaca gggaaactgc
     8401 atgttcatcc gtgatatcca gctcgtcctt atcggtttcg tataattcat attccatcat
     8461 gtctaacaac ctgatcaacg cctgccccat gtgaccgagg gaaccgacga tccatatttt
     8521 tttcattgta tacctccttt attttatata atgttgggtt ttgtagaaat gatcaagaaa
     8581 atatctggga acagtatagc atacgatacc gtgcgaaaga aagcacaaaa gagaagtgag
     8641 aataatttcc ttggcatata tagactgacg atatatattg tgtgtagaaa gcccatatag
     8701 agaggagcgt atacatatgg caatcgatag gaatctggta cagggaagta tggcaatgat
     8761 gatcctgcgt ctattggaga ccaaggacat gtatggctat gagatgattg aggcactgcg
     8821 agagagatct aacaatgtct tcgaactaaa ggccgggaca ctgtatcctc tgctgcactc
     8881 gctggagagc cgtggagatg tggtgtctta cgaggacaat acttccggca agacgagaaa
     8941 atactaccag ctgactcctg caggaaaaaa gcatgcggaa gaaaagaaga aggaatggca
     9001 ggaataccag actgcagttt ccggagtgtt gagtcttcag agagggtgct gattatggga
     9061 aaggaagaaa aaagggtagt gctggcagcg catgaggagg cagaacaacc ggcggcggaa
     9121 gagaatacgg attggcggaa tgccggaagc atccgggatt atctggatgc cataggagaa
     9181 cagatcgaga atccccatgc cagatgcgca atccgcaggg agattggcaa tcacattgag
     9241 gaacagacgg aggactacca ccgggaggga atgacacccc tggcagccgc acaggaagcg
     9301 gtacgccaga tgggggatcc gctgcagacc ggcagggaat tgaaccggat ccacaggccg
     9361 aagtttccga cagtgttgtt tggcatcacg gtgatactga cactggtggg aattgtcatg
     9421 cagggaatct tgttttccca tatggagacg gtgggagcgg aaaattatct gcgtaatacg
     9481 atcctgtata acggaatcgg actgttgctg attctcggac ttctctatgg aaactatatg
     9541 aaattggtac gtttcagcta tgggctgacg gcgcttttta tagctgcagg gctggcaggc
     9601 agtatgctgg ggacacatat gtggagtggc agttatcatg acagatacga agctgtctat
     9661 ctgctgtgga tgtttcttcc ggttctgtat gccttgcttt tgtatcgcat gcgaacctta
     9721 agctggaagg taatcctgat tctggatgcg gtgtatctgg cagcggtgct gctgttatcg
     9781 aaggatatta cctgctctgc cggtatgatg gaagcattct tgttatctat cgtaattttg
     9841 ttccttgcca tcaaacgggg cattctgccg ggaaaaaaga gattactcta tttggcggcg
     9901 gatggattac cggatgggaa aaggggagac cggttatatt gccagcaata ttcagcagga
     9961 acagcatggc ttttcattgt tgggtggggg aaagatcagt gaggcattac tttttacctc
    10021 agatcaaaat aacgggacat ggtccctgtc acaggagagc tgtacaacct acattattca
    10081 cagcattttc ctgtggtttg gtatcattgt gggagtattg gtgatcgtgg cactgtttgc
    10141 ttttgcgctt tatgcactcc gtgtcgctct gcgtcagagt aatcgtgcgg cattgctgtt
    10201 gggcagtgtg tgcagtctga gcatcctgct tcggatcgtc aatgcggttc tggtgaattt
    10261 tggatttggc atttactaca ccatatccat tccatttctg gcttatgggc tggggaactg
    10321 tctggccaat tccctgatgg tgggagtgat tttatgtgtt tttcgtaaca gcaatgtcat
    10381 gatggaagag ctggaaagaa aaattagtat acaaactgcc gatataaaaa gaagagaaac
    10441 aaaaacagaa tcgtaaactt gtataggtac aaaggcgtaa tttagaattt catattcata
    10501 gtgcataaaa aacccccttc aataagaaag atagtcccct tatctttgaa gggggctttt
    10561 gctatactgt taacataaag ttaaggggaa cgcaaaaaca gctaaaatta gcctatttat
    10621 taatggaagg gaaggacgaa gagaaggtat gagtgaaaaa gaagtcatag tatccatgaa
    10681 ggatatatgc aaaagcttcc cgggagtaaa agcactggat catgtacagt ttgaattgcg
    10741 atccggagaa gtcatggccc tgttgggaga gaatggcgcc ggcaagagta cgctgatgaa
    10801 gattctgagc ggtgtgtata ccagagacag tggaagcctg gaaatctttg gcaaggagta
    10861 tggggatctg acaccgaaac aggcacagga agccggagtt gctattatcc atcaggaact
    10921 gaatatgtgt aagcacctgt ccgtagcaga gaacatgttc ttaggcaggg agaagacaaa
    10981 aggtattgta ttggcaaacg gtgatatgga agcggaagcc tcccggatat taaatgatct
    11041 gaagatcgat attgacccga agcaggtcgt gggagaactg ccggtatcca aacagcagat
    11101 ggtcgaaatt gccaaagcat tgtcggtcaa tgcgagaatc ctgatcatgg atgaacccac
    11161 atccgcactg acagcaaagg aaatagatga tctgttccgc attattaagg atctgcgatc
    11221 caagggatgc ggtattgtat acatatccca cagactggaa gagcttcagc atattgtaga
    11281 ccgtgtgacc atcatgagag acggacagta tattacttcc atgaatttca aagataccac
    11341 gatggaccag attatagcca atatggtagg acgagagatc aaggaacagt tcccgagaga
    11401 gtcgtgtaaa aaaggggaaa agattctgga agtcaaggga ttaaacgcag gtcgcatggt
    11461 gcgggatatc aatttcgcat tgtatgaagg cgagatcgtg ggctttgccg gactgatggg
    11521 tgctggaaga acagaaacga caagagcaat cttcggagtg gatccaaagg aaagcggaga
    11581 gatcattctg gaagggaaaa cattaaaaat cacaaatccc aatgatgcaa tccgcgcagg
    11641 aatcgtactg gcaccggaag accggaaaaa ggatggattg tgtaccaaac tgagcatccg
    11701 gcacaatatt gcattaccta atctggatat tctctgcaac aaactgggag tagtcagcag
    11761 caagaaggaa gatcagatgt gtgacgaggc agtagaaaac ctgaagatca aaacaccgaa
    11821 cgtggacatc aatgccggta acttatctgg tggtaatcag caaaaggtag tcgtaggaaa
    11881 atggctggca cggaattcaa aagtcgttat cttcgatgaa cccaccagag gaatcgacgt
    11941 ggcagcaaag gttgaaatct ataacctcat gaacgaactg aaaaaacagg gaattgccgt
    12001 aatgttcgta tcttcggaga tgccggaagt catgggaata gccgatcgta tcattgtaat
    12061 gtgtgatgga aggattactg gggaattgga tgccagaaac acaacgcaga atgagatcct
    12121 gaccctggca acccagttcg agaagaagat cgcagctaac gcttaaatta taaccataag
    12181 aaaggtgaaa atatcattat gaaaaagcaa agtccgttga aaagaatttt tgcgatcaga
    12241 ggaatgggag gagcccttac cgcttttgcc ggtctgatca tcatctatat cgcttttggt
    12301 attataaatc cggccgtatt ttccggtcag aatgtaatga acctgttacg atccatgtca
    12361 aagtatctga tcattggtat tggtcagagt tatgtgctga tcaccggtaa tatcgatctg
    12421 tccattggat ctgtggtagg tatgagtgcc atgatcgcag caacactgat gaccatgggt
    12481 gtgaaccctg tagtagctat cctgattact tttgtatgtt gcatgatcat cggggtactg
    12541 aatggtattc tggtaggtaa gtggaaactg cctcccttca ttgctactct gggaaccatg
    12601 tttgtagcaa gaggtgttgc ttacatggta aacggcaacc gtaatacgga tgccatcgca
    12661 tccggtgtcg gcaaagatgc cggagagact ttccagaatg ttttctacta tggtaaaaca
    12721 ttgggcgttt acaatacttt ctggattgca attattatct ttatcgtatt cttctttctg
    12781 ctgtccaaaa cacgtaccgg cagacatatt tatgccatcg gttccaatgt ggatgcagca
    12841 aagctgtccg gcgtcagcgt agtaaagacc accaccaaga cctatctggt cagtgcattc
    12901 tgttcttttg tggtaggatt gatcctctgc ggtcaggccg gaatgggtaa catggaagcc
    12961 ggtaacatgt acgagatgta tgcggtagcc gcaggcgtta tcggtggtat ttctcctttg
    13021 ggaggaaccg gattgctgtt gggaactctg gcaggtgctt ccgtatggca gacactggag
    13081 aatggcctga atatgattgg cgcccaggta ggtatccaga gactggttat cggtatcatc
    13141 gtagtgtttg cagtacttct ggatgttgtt gtaaggaatg gtgtatttaa gaagaaaccg
    13201 gctaagaatt agacatcaat taagatgcta agataaatca atgaacactt ttaaggaggg
    13261 tttacaaaat gaaaaaggta ttatcagtag cttgtgcatt atgcatgaca acagcaatgc
    13321 tgaccggatg tggcagcagc aacaacgcag gaaccgcagc caccggtgag gcacagaaca
    13381 gtgcggcagc atccaccacc acagagcagg cttcaactgc aacagacgca ggaaccaccg
    13441 caggatccga tactctgatc gcgctgatca ccatggattc tctggatcag cactgggtaa
    13501 ctctgaatga gggcgcacag aaggcagcag cagagctggg tgtaacagtt cagtttatgg
    13561 ctcccaatac caaggatgat gctcagcaga tcgagtgtgt aaacaacgca gtcagtgcag
    13621 gtgcacaggc tatcatcgta gcagcaaacg gtcccgatgc gatttcttcc gcactgagag
    13681 aggcatcaga tgcaggcgtt aagctgattt acgtagattc ccctgcgaac gtatccgcag
    13741 aagctacttt ctctaccgac aacaatgctg caggtaagac agcaggtgaa gagatgaaga
    13801 aagcattggc agatgcaggt gtcaccagtg gttccattgg tatcatcaat gtaaatgcag
    13861 caaccgattc ctgcgttatg agagaagaag gtttccgttc tgcatttgaa ggaactgatt
    13921 tcgaacttct ggagactcag tacggcgaag gcgatgcagc aaaatcccaa agtattgcag
    13981 agaactatat tacccagggc gttgtaggta tcttcggatg taacgaaggt tccaccaccg
    14041 gcgccggtaa cgccatcaag gcttccggta aggataacgt aatcggtgta ggatttgata
    14101 agtctgatgc tatcctgggt ctgatcgatg acggatatct gctgtgtacc atggctcaga
    14161 atcccgatgt tatgggttat gaaggtgtaa aagcagctgt aaaagccatc aatggtgaaa
    14221 gtctcggtgg tgctgtaacc gatacaggag tatctgttct gaccaaacag cagtaagcaa
    14281 aatcttttct cttccataaa tataaaaatt caacaggaat atccggaaat atgggatatt
    14341 cctgttgaat tgtatttaaa cgagtgctaa aataaatcta tatttatgga aaaaaggcta
    14401 ataatgaggc taaaattatg tggaaggtaa tgatcgtaga tgacgagaag ctgatctgca
    14461 agcttgtcca ggcattggtc gagtgggaca aactgggaat gcagatggcg gcacaggcag
    14521 aaaatgccat acaggcactg gatatgctgc agcaatatag accggacatt ctgatcacgg
    14581 atattcgcat gccgggaatg gacgggctgg agctgataaa aaatgcaaaa aagatatgtc
    14641 cggaactgga aatcatcatt atcagtggat atgcacattt cgagtatgca cgaaatgcgc
    14701 tttcccttgg tgtgggaaat taccttttaa aacctattaa acaggacgaa ttgaacgaaa
    14761 cactgcgaaa aattggtgag cggctggatg caaagaaatc tgcccagggt atggaaatgg
    14821 ctgagcggag agtcagtgag agcgatgtaa atcgcctcag atatgcgttg ctgaaggatc
    14881 ttgtagaaga tgcctataat ccaaccgcag tgcagctgca ggagaactat tattttaagg
    14941 cggaagctga ttgttatcag gccatagtgg tgaaagcagg atatgatccg gaacaaatga
    15001 gtaaggcagc acagactgtg atctgtgaaa aaacgaaaga gttgttctat cattgcctgt
    15061 caaaaaactg taatgactgc ctttttgatt atgtgaaaga ttccgggtat ggtattctta
    15121 attttataaa agaagatacg gataaaataa gaactttgct tttggaattt ttgcgacaac
    15181 tggaagcgaa ccgttccatg ttgggaccgg taagattttc acttgccatt ggcggtgtga
    15241 cagtggatgc ggaacaattg accgcatcca taagaaaagc agagcttgcg attcgggaga
    15301 ggatcgtgga aggctgggga agacttctgg aagaagtacc tccggcatcc ggattaccaa
    15361 tgcaggatat tctggatcga tattgtaaag aaatggaaca tgccatagaa gttctggatc
    15421 cggcagaagc cagcaagtgc ggagaagaac tgaaaaatgc ggtgatgagt gtaccggatg
    15481 tcagaggacg ggaaattcag gaaacggtgc tgtcagcagg aagatttttt attgtgcgtg
    15541 taagagctac atctcccact atatttgaag aaaagtgtat gcattgtggc tctgcggaag
    15601 aattgtttca tgaactgtca acgctgcagg aagaactgat gacagaagct ctggaagcca
    15661 gacaggggga gacttcccgg ccgatccgcc aggcaaagca gtatgttcac aaacattatg
    15721 cggaaaatat tacattggaa gaagtatgtg accatgtggg tttcagtgta gcatatttta
    15781 gtgccctatt taagaaggaa acaggagaag ggtttgccaa atatctgatg cgggtaagaa
    15841 tggatgaggc taaaacgctg ttgagagaaa caggattgtc tgtgaccgag atctgtgagc
    15901 gggtaggata taatgacaga aagcatttta cccagacttt tcataagatt gccggcgtaa
    15961 atccggcaga gtaccggaag ctgtacggtt gagaggagag cgtatgaagc agggaaaaat
    16021 gcattatctg aactttcgga cattgtgtgc gtggagtttt atggtgttgt ttctggctgc
    16081 gctcttggga tatcttatct atgaaaaaat cagggggaat ggggcaggct ggttcccggt
    16141 gatctttgga gggtgcctgt tggtgcttgg tatctgctat gctgtagtgg agatatggct
    16201 gccttaccgg cgtatcgaga agcggataga attatttctg gacggttata ccaccagcaa
    16261 tctggtggga gaagatggga tccacatttc tccggagctg gagagtctgg tcagcaaaat
    16321 gcagtatctg atgcagtcac aggagatgct gaacctgaat aagagacagg cacagtatct
    16381 ggcattacaa aatcagatca atccccactt tttatataat acattggaga gcatccgtag
    16441 tgaggcactg attgcaggac tggtaagtgt cgcagatatg acagaggcat tggccacttt
    16501 tttccgctat accatcagca aagtagaaaa tctggtgtca gtggaagaag aattgcagaa
    16561 ctgtatgact tattttaaaa ttcagcagta tcgtttcggc gacagactgc agctttccgt
    16621 tgaatgtgat cccgaggaaa aagcagcgat ttacagctgt cgtatcccca aactgacctt
    16681 gcagccgatc ctggaaaata gtatcattca tgggacagag tgcaaactgg gaacgggaca
    16741 tatccggatc ctactggaaa tgacggaaag ttgtctgctt ctgtgtatct cggatgacgg
    16801 caacggcatg gaagaacgca aagtagcgga tatcaatgaa agattgcaaa aaagcggagg
    16861 tattctcgca acgggagaat ccgagacaaa aggcgggatc gcactggtaa atgtcaataa
    16921 ccgtatccat ttactatttg gagaagaata tggaattcat gtgtattcta tgccggagat
    16981 tggcacggat gtgaagataa gaattcctgt aatctacagt gatcgtaatc tgattcatat
    17041 ggaagaaagg caggaaaact actgttgatc agggaagtgc tgcgcatgga gaaggtgacc
    17101 tacaaggaac agggaagaat tctcctggat aattttaata taacggtacg aaagggagag
    17161 gtattcggtc tgatcccggt caatcgttat ggaatggatg cttttctcaa actgttgcaa
    17221 caaaatatgc cgcttcacta cggatatgta tattatcgtg agcaattggt caatcagtgg
    17281 cagaggtcgc ataaccggac gaacaggatc agtatcatcc gcaacaaaag cagtctggcg
    17341 gaggatctaa ccgtggtaga caatattttt gtcctgcgga gaggatttca taagtatctg
    17401 attcatccgg gaatgttgga acgactgctg cagccttttc tggatgaaat cgggatcaat
    17461 atcccggcgg gggcttatgt cagcgaactg acaatatttc aaaagtttgt gacagaagtt
    17521 ttgaaagcgg tggtggcagg aagtcagctg atcgtattgg aaaatgtcag tacttttatt
    17581 agcgagagcg aactggagga attgcacagg atcatacgac tgtacgcaga aagaggaatt
    17641 tctttcattt acatcacggc tcattttgaa gaagcaacct atctgtgtga tcgtatggca
    17701 ttaatggtaa acgggcagat catcaaaacg tttcagagag gtgatccgat tcctgataca
    17761 tttccgctac agagtgtgga ggaatatgac aggtgggtga gatctcaaca tagacgacag
    17821 gaagcagata cagacagaca gaccgtattg atcatggata agatacaata tggtaatctg
    17881 aaagatatta cgattcgggt cgggcaggga gaatgcgttg tattgcagga tctggataat
    17941 cgttgcattg ctgattttgt ggagctgttt caaggagaga ttccagagaa aggaagcttt
    18001 tatctgacga aagggacaca aaaaagtgag aaaaacagag atattgcggt aatccaggag
    18061 ctacccacag agactatgct gtttcctcat atgagttatt ttgataacct atgttttacc
    18121 atggatcatc gaatccgggg tgtgtggaat cagtctggaa tccggagaag tatccaggag
    18181 gaacaaaaag atctgttggg ggaggacgta tttgacactc cggtgtggaa cctgaccagg
    18241 cagcagaagt atgatctggt atatacgaga attctgatgc agcatccgca ggtcgtgttg
    18301 tgcgtacagc cgtttaaaaa tgcggaagtg tccgtgcgca gtcatatctg ggagctgatg
    18361 gaacggtttt tgaaaaaaaa tattgcagtg gtgatcctgg cggtgaatct tgccgattct
    18421 ctggctctgg ctgaccgtct gatccggata cggaatggac agattcagga aatctatgat
    18481 caaaaggatt tctccagatt gccagagcat acaccctggc tgcatttata tcagaaaacc
    18541 tatgggaata gatcggaaga taaaaaataa aaagaaagaa ggaatcacta tgaaattggg
    18601 agcattagaa gcgggcggaa caaaaatggt ctgtgccatc ggagatgaaa caggaaagat
    18661 ctatgagcag gtatctatag ctacaacgac gccggaagag accattccga aattgatcgc
    18721 atattttcag gacaagcaga tagacgcatt gggaattgca agttttggac cggtggaact
    18781 ggatccagca tcggaaaaat atggatatat tactaccaca tctaaacttg catggacaaa
    18841 ctttgatttt ttgggaagca tgaaagcagc gttaaaatgt cctatgggat ttgataccga
    18901 tgtaaatggt tcagtactgg gtgaggttac tttcgggcag gcaaaaggga aacattgcgt
    18961 catgtatttg accgtgggaa ccggaatcgg agcaggcgtg tatattgacg ggaagctgct
    19021 gcacggtatg cttcatcctg aggcaggaca tatcctcatc acaaaacggg atacagacca
    19081 ttatgaagga aaatgcccct atcacaagga tcattgtctg gaaggaatgg cggctggtcc
    19141 tgccatagaa gccagatggg gcaaaaaagc agtggaactg gcagattgtc cggaagtatg
    19201 ggatctggag gcagactacc ttgcgcaggc attgacaaat tatgtgatgg tactttctcc
    19261 ggaaatgatc attctcggag gcggtgtcat gcatcaggag caactattcc cattaatacg
    19321 caaaaagctg attacctata tcaatggcta tgtgataaca gaagaactgg aggatccgga
    19381 gcattatgta gtacctgcgt cactgcatga tgatcagggc atcatgggat gcctggaact
    19441 ggcgagaaga gcattggagg gataatctat gcctttatgg aacaaaagag cggtaagcag
    19501 cggacaaaca gaagaatcat ataaggaaca gctacaatcc ttgaaacaga ctgtcaataa
    19561 tctgggattg gtatccgaac aatttgaagg tgcggcagag gggctgacag agtcctccga
    19621 taaggtggga catggcatac aggtgcttgc ggaaggtgcc tcaaggcagg aaagagatgt
    19681 attgcaatgc cggaaaatag ccgaggaatt cacagcgaag atcacggata tggatcagga
    19741 gactgcaact atgcagacac aggttcagca gatgcagcaa cagagtgatc ttggcagaca
    19801 aagagtggaa catctgggga aaacacagga aaaactcaaa ggttccatgg atacgatcac
    19861 agaagagatt cgtacgcttc tggataaaaa tgcaaagatt gaatctgtca caagtgttct
    19921 ctatagtatt gccaagcaga cgaatctgct gtctttaaat gcatccatcg aggctgccag
    19981 agccggggaa gccggaagag gatttgctca tgtggcatat gaagtacgaa aattgtcgga
    20041 ggaatgtcat aaggcaagtc agagtatcaa tgcatccatt caggagatta taggttcgtt
    20101 aacaaaactg aaagagatca tggaggaatc cgatgcagct tttgaagcac agaaaaccgc
    20161 ggtaggggat acaatttctt cctttgaaaa tataaatcac agtgtgaaag aactggcaga
    20221 agcgcagttt tttttcacag aacgtatgga tgaagtcaat gggaagaagg aagagctact
    20281 ggaagtcatg gaatctattg caacaatctc cagtcaggca tcggcatcct cggaaaacgt
    20341 ggcgggaatt actgcagaac agaagcagaa tgccagcctt atggggcatg tatccgaacg
    20401 attgaacagc gaattaaatc agctgaatga aatcctggct aatataccgg tggattatgc
    20461 accggtacag cgcaggaaaa ttgccatggt atgggatctg gatgatcctt tctggtatcc
    20521 ggcgacgaaa caggcctatc gcactgccaa aattctgaat tttgatatta cggtatttgc
    20581 acctaaggga agaggagaag caggaactct ggaaatggtg cattttctga aaaaggtgtt
    20641 ggaggagcgt tatgatggaa tctgtatctc acccattgcg gatctcagag tggaaaaatt
    20701 gctgcagaag atggcagaga agggtgtgga ggtgattttc atcctgtctg catttgatag
    20761 tgttccctat cgcacactca tcggaactga cagttatcag tgcggacgga gtaccggaga
    20821 ggctgtgaga gaagccttgg gagccgacgg tgccgttggc attatcaagt ggaaggataa
    20881 cctcattgag actgtagagg acagatacca cggtgttgta gatgttctga aagaaaacag
    20941 aattccttat tatgatatga ccggtccggg agaacctact gctgaagaag caacaaaatt
    21001 aactgaccgg cttcttacgg agcatccgga tatctctgta ctgtgcgcta caaatgtagg
    21061 atggggattg gcttttgcca gatatcttaa gtccaaaggg aagaaggtaa aactggtaac
    21121 agtggacttc acggatgaag ttggagaatt catgaaggcc ggctatgtag atgctgcaat
    21181 tgcgcagcgt ccggagagct ggggatccat gacactggaa aaaatagagg cagtatttga
    21241 aggacagagt atacccaaaa ccattgacac cggaacctat ccggtaacac cggccaatat
    21301 cagaatttat gtgaaggact gatcccgttt cttgcggtat tgcagcggcg acatccgaaa
    21361 agaagcgtga aagctacgga taaaatgact tacattagaa aagccggact gcagggcaac
    21421 ctctgtaacc ggcagagagg tactctctaa cagatggcag gcatgggtca gtctcagatg
    21481 catcaggtat tgcgtcagag tcagatgaaa atgctgctta aaatagcggg acaaatattt
    21541 ctcggataaa tgaaattgct ctgccagggt ttccaaccga agggggtccg tgtagttata
    21601 ctgcagataa gtcagtatct cacgctgcat tggatcgttc cttttggagc ccatcgggtt
    21661 aatggttccg caggcagcga ttttttgcag aagtgacaac aataaaatac gtaatcggaa
    21721 atgcccgctc aaagtatttg gaagctgtct gacagacagg atctcacgga tgagagaaat
    21781 gatgtcagga cagctttttt cgtgggaaag attatgatgc aaaagcaatt catggttacg
    21841 caggggaagg agaaactccg tttccaacag atcgtccgtc tgaaaggaaa taaattccag
    21901 tggaaacaac agggtatgat agtctacgcc cggaattgcg gaacccataa aatgaagctc
    21961 accgggattc acaaagaata cggagccgcc ggccgcttcg tattcttccc cttcaatgat
    22021 gacattaaga gagccggagc ggacatatat gatctcgacc tccggatgcc agtggacagg
    22081 aatctgaaaa aaagcaggca tatcatgcag gtgataacag ctgaaaggaa attcttttgt
    22141 tccgtgaatg cgttcttctc ttaattcagg atacataggc agacctttct gtgaagtgga
    22201 gtagaggata ttatggtcag attactataa aagtagaaat aagtcaattt tctgtggtaa
    22261 aaatgcaagt tttaggaaag ataaagtagt aatatgtaaa aattcaaggg actattcgga
    22321 atagtattct gaatagttgc aaaatgaaga tgcaaattta taggaaccgc agaaaggata
    22381 taaaacctac gatggaagga cagaataagt cattatttca attatttgta ccactgtgtc
    22441 tggagacgat cttttacatg ttgtccggaa tggtggatac tttaatgctt tcttctgtgg
    22501 gagatcaggc agtcggagca gtcggaacag caaatactta tattggtgta tttattatta
    22561 tgtattcggt agtttctatg ggaatgctgg ctgtgatgac acagaatatc ggtgcgggca
    22621 gaccggggat cgcctgtcag gcgaagactc tgggattatg gtttaatggg atcctgggtg
    22681 ttgtgatgtc tgtgtttttg tttttctgtt ccgatgggat tctgtatgtg gtaggtattg
    22741 caccggcatt gaaggagcct gctgtacaat atctgcggat cgtaggaggc ggaagtatac
    22801 tgaacgcaat gattcccata ctggcaggat atctgcgtgc gtttggatat acgaaacagc
    22861 ctctgatcgc aactgtgaca ggtaatattt tgaattttat attaaatgcc gtgtttctgt
    22921 ttcgtttcca cctcggtgtg cagggggtcg catccgcaac agtgatctcc aaagtagtga
    22981 atctgatatt gatagtgatc tttgtacggc ggctgatccc tgcaaaaaaa tattcggaga
    23041 gagtaaattc cggagagatt ttgcggaaga tcatccggat cggattacct tccgctttgg
    23101 agaatattat ttataatata gcgatgacac tggtgatccg ttttctgaac cagatggata
    23161 cagaaggaat caatgtggct gcccgctcct atacagcaca gatcacgaat ttatccttct
    23221 gtgtgggaag tgcatttgcc caggcgaatg ccattatgac cggatggtac atgggtgcgg
    23281 gaaaatatga aatatgtgat aagcagacta aaaaatctgc ggcgatcgga gcggtactgg
    23341 cgatgggaac agaatttctg tttgcagtca gtgccccggt cctgatctct tttttcacgc
    23401 aggataagag tatggtagaa ctagtgcaga agctgatgtt tattgatatt gccctggaag
    23461 ccggacgagt cacaaatctg gtatacggga atgctctgaa aaccagtgga gatgctgtat
    23521 ttccggtgat cctcggagct gttttcatgt ttttgtgtgc agcgggagga acgtatctgt
    23581 tggggataca gctgcatctg gcagtagtgg gtgcttatat cggtctgaca gcagatgaat
    23641 gctgccgtgc ggtcggcatg atgatgcgct ggcggagcgg agcatggaag aaaaaaggat
    23701 tgatcgaaga ttgatggaag tgatccggga gtactataag acggtatacc ggctggcttt
    23761 tgcccaggtg gacattctct tgcttttcgt agcttatcat agcatagcgc attttttata
    23821 attaaaatat tgacaatgtg aaaaaatgtg ttatagttct aaatgcaaat gcaactcatt
    23881 tgcatttaga acaaatatat gattaagtat tcatttgata aagaaagtat ggaaaggtaa
    23941 gagttatgtc ggaagttatt ttagatacct taatagatag tataaagctg gtgccgtttt
    24001 tgttcctgac ctatctggct atggaatatc tggagcataa ggctggcgag aagaccacac
    24061 atatggtgcg aaaagcaggt aaaatgggac cgttgatcgg tggcgtggca ggtgtgctgc
    24121 ctcagtgtgg attctcagca gcggcatcca acttatatgc aggcagggtg atcaccctgg
    24181 gaacactgat agccatttat ctgtccactt ctgatgagat gctgccgatc ctgatttcgg
    24241 agaaaatgga tatccgcttt gtactgggtg tgctgggagc gaaagcagcc attggtgccg
    24301 tagccggatt tgtgatcgat ctgttgatcc gggagaggaa gatacatcct catgaccacg
    24361 ttcacgggca tgaggaaaat gatcatgaag aggaagagca cattcatgaa atctgtgagc
    24421 atgagaactg ccattgtgaa aaggatggca tttttctatc tgcggtaaag catacgcttc
    24481 acattacctt tttcattata gtcattggat ttgttctgaa cacagcactg cattttgtgg
    24541 gagaagatgt tctggccgga ctgatcctga atcgtcccgt actgggacct gtgctggcag
    24601 gagtggtggg actgattccc aactgtgcag catctgtgac cataacgcaa ttgtatatca
    24661 gtggtgtgat cagccttgga gccatgatgt ccggattgct ggtgggcgcg ggagtaggac
    24721 tgttggtgtt gttccgcgtg aatccggata aaaaggaaaa tctgaaaatc gtgggaattt
    24781 tatatgtgat tggcgtttta gcaggaattg tgataaactg gctgtaggca caagcagtgc
    24841 caaaattgag agctgactct ttgactgctt gcaggcataa gaggcagatc caattttgat
    24901 agggcgacgc ccgtccagtg agggaacgca gggcgttacc gagctgagca gcactgcgga
    24961 tgccccggat cgcgatatta aaggacagat atctatggaa gaacataaat gtgaacacaa
    25021 tgatatttcc taccctaagg gattgaaatg gaccaaacag agaaaatgcg tgtatcaggt
    25081 actggaggaa gcgaccgaac ccctcagcgc cgtgcagatt tacaatcgta ttgagaaaga
    25141 acacgaaggc agcgattatg ctgtatccac aatctaccgg atcctggcaa catttgaaga
    25201 acacggaatg gtagagaaga ctacgttcat gggagacggt accgtagtat acgaattgaa
    25261 ccgtggggaa catacccatt atgcggtctg cctggaatgt cataagcgga ttccgctgcc
    25321 cagctgcccc tttgcacacg tacatctgga acaggatacc ggagatttta ccgttaccgg
    25381 acacaaatta gagctttatg gctattgcaa aaagtgtcag gaagcccgtg aaaatcacta
    25441 atttcaccta ttatataaaa aaatcgaaaa aaatatttaa ttttttttga aaaagtactt
    25501 gcattattta aaatcatgtg ataacatatg tcttgtcgtt gcgggagaca caacaacgac
    25561 aaagaaaatg caccgctagc tcagttggta gagcacctga ctcttaatca gggtgtccag
    25621 ggttcgagcc cctggcggtg cactcgagag aatcgttttt ggagacatag gagctccggg
    25681 gacggttctt ttttttggtt ttaattttgc tggacaaact actttacaaa gctgggaaca
    25741 catgttatat tgaaattagc caaagagaga ctaatgtcta acttcaagac gcagaaaacc
    25801 ctgggatgct accccggggc tttttgttgc caggctgact gttgctattc atcaccgtcc
    25861 agccatttgc atatgtagta gccgactaca cttgccacaa cagagagtat aaagttaaag
    25921 agttctacca aaatgtcacc ccttccacag tctggttgga aatgggaaac gacatggatg
    25981 aaggacacta cagaaaaatt gttaatagtt ctgtatatga agtattcacg atatttgaaa
    26041 ttgatgagtg aggggaatac tatgaatgat gcaatagcaa tgatgttttc aattttaatt
    26101 gtcatgttcg ggtacctggt atttaagatc cggacagatc agtcccaatc aacgaccgcc
    26161 agcaccagaa aacggagcaa gaagagaggg acaggaaagc cggttatcaa aaataactgg
    26221 aatatgcgac cactggacac aaaagatcct gcagacaaag atctgcggta tgaggatttt
    26281 gtagaatcat cttggaaaga aatagaaaaa taattgtctg acaaactact ttacaaagcc
    26341 tggaacccgt gctatattga acttagataa gaaagtaata attgttccaa ggcgcagaaa
    26401 accccggggt gcgatcccgg ggctttttgt tgctaggctg actgttgcta agcttctccg
    26461 tccagccatt tgcatatgta gtaggcaact acacttgcta taacggagag aaagaaagta
    26521 ataataatat ccaaagcgcc acctccttcc tgctggaaag agtcaacagc atgcctatta
    26581 tatcacacgg ttctcgtagc gtaaaccgtc aaaaaacgac ttaaaatacc ccttagatac
    26641 catttctctt tatcagggtg tctggggttc gagctcctgg cgatgcactt taaaggatcg
    26701 tttttggaga cataggagct ccggggacgg ttcttttttt gcatagatac cctgattaag
    26761 gagatttcac atgactgcga tatttgatac gcatgcgcat tatgacgatg acgcatttaa
    26821 cgatgacaga gaacagcttc tggcggcgtt gccggggcag ggcattgccc gggtggtgga
    26881 tgtgggtgcg agcctggcat cctgccggaa agtgttagag ctgatggagc agtacgatta
    26941 tatatacgga gccatcggcg tacatcccag cgagaccgcg gaactgacgg aagaatctta
    27001 ttcctggctg aaggaacagt gccagaaaga gaaatgcctg gcagtgggag agatcggact
    27061 ggattattac tggccggagc cggaccatga gacacagaag aagtggttcc tgcggcagct
    27121 ggatctggcg cgagagatcc gaaagcccgt tatcattcat tcccgggatg cggcgaagga
    27181 tactgtggat ctcatgacgg aggcgcgcgc ggaggagatc ggcggtgtaa ttcactgtta
    27241 ttcctatacg aaggagacgg cgaagatttt cctggatatg ggattctact tcggaatcgg
    27301 cggcgtgctg acttttaaaa atgcaaagaa gctgaaggaa gcggtagagt acattcccat
    27361 ggatcgcatt gtattggaga cggattgccc gtatctggca ccggaaccga accgcggcaa
    27421 acgcaacagc tctctgaaca ttccctatgt gatcgctgcc atggcgcaga tcaagagaat
    27481 cacagaggaa gaagtgcgca aggcggcatg ggacaattcc ctgaaattgt atcgcatgga
    27541 taaataaccg ggtaaaaagc aatggaagca ataaaagaaa tggaagaaag caagacaaaa
    27601 cagcaactat tttcaaaaga agatctgaaa aaactaatcg ttccgctctt atttgaacag
    27661 gcgctggcta ttaccgtggg tatggcggat acggtcatga tctcttcggc gggcgaagcg
    27721 gcggtctccg gcgtatccct gatcgacatg tttaataatc tgatcatcag tgtgctggcg
    27781 gcactggcta ccggcggagc cgtggtgacc tcccagttcc tcggagccgg gaaaaaagag
    27841 gacgcctgca aatctgcaaa acagttgatc gtgtcctccg tatgcattac catcggtatt
    27901 tcgattttgg tcatggtgtt ctgtaatggc atcatttccc tatttttcgg taaaattgaa
    27961 gcggacgtat ttcataatgc tgtgatctat atgatgatct ctgcactgtc cttcccgttc
    28021 cttgccctgt acaattcctg tgcagcgctg ttccgctcca tgggaaattc caagatcaca
    28081 ttgaaggtct ccgtggtgga aaatatcatt aacatcggcg gtaatgcttt gggcgtctat
    28141 gtactgggac tgggcgtggc cggtgtggcg attccttccc tgatctcccg tgcagtggca
    28201 ggattcatcc tgtatcattt gctgaaaaac ccgtccaacg aagtgcattt attaaaagaa
    28261 agattccaca tcgactggcc ggtgatccat aagatttttt acataggtat ccccagcggg
    28321 attgaaaacg gtattttcca gctgggacgc gttatcgtgg tgagcattat cgctggcttc
    28381 ggaaccgtgc agatcgctgc caacggtgtg gcgaacagtc tggacagtat gggctgtatc
    28441 gtgggtcagg ccatgaatct ggccatgatc acggtgatcg ggcgctgcgt cggtgccggt
    28501 gattccggac aggtacggca ctatacgaaa aaattattga agatcacgta tgtttgtacg
    28561 tttgtggtaa acagtatcat tctgctgtgt ctaccctgga tcctgaaatg ctacggatta
    28621 agcacggaga ccacgcagtt gtcctacatt ctggtcatga tccacaatgg catggcaatg
    28681 tttttgtggc cagcatcttt tgtactgccg aacatgcttc gtgcctgcaa tgacgtgcgc
    28741 tttaccatgg tgctgtccat attctctatg ttcatgttcc gtatcggatt cagctatatc
    28801 atcggtgtga acatgggtta cggagccatc ggtgtctgga ttgccatggt actggactgg
    28861 atcttccgcg tgatctgctt cgtgagcaga tatttgtccg gacattggtt gaagacagcc
    28921 agattgtcag agagatagcg caccggttgt ccttgtatag gaagtctgtt ataataccga
    28981 taacagatag agatctgtac aatatcagag gagaggtacg tatcctccga caaaaggaat
    29041 cgtctgcgga atggaggtta atatgcaggt agtattggaa aatgaagctt taaaggtaac
    29101 gatcaacagc tttggcgcag aacttgcaag tattcggggg aaagagacag atactgagta
    29161 tctgtggaat gcggatgcaa aattctggaa gagaagtgca ccggtactgt tcccctttgt
    29221 gggcagtctg aaaaataagg aatatcacta cgaaggaaaa gcctatccca tggggcagca
    29281 tggttttgcc agagatatgg agtttgccgt ggatgtacag atggcgacgg aagcatggtt
    29341 ttccctgaga tctaatgaag aaaccaaaga gaagtacccg ttcgagttta ttttaaaagt
    29401 aggttatgaa ttagaaggaa gaaatctaaa ggtcatctgg caggtggaaa atccggatac
    29461 aaaaacactg tacttttcca tcggcggaca tcccgcattc atgtgtcctg tgaatggaaa
    29521 aggaaagcag tcggaatatt attttaagtt tgatgccgac aaagatctga cttacggact
    29581 ggtggcggat gacggacgag gactcatggg acagcaaaag gatgtactgc cggtgagaaa
    29641 cggctatgcg cagatcacag agcatctgtt tgacagggat gctctgatcg tagagaatcg
    29701 tcaggcttct gtagtgagcc tgtgtacccc ggacaaaaag ccttacctta ccgtaagctt
    29761 tgacgctccg ttattcgggc tgtggtctcc cgcaggcaag ggagcaccgt ttatctgcat
    29821 cgagccctgg tacggcagat gcgaccggac gaccttcgac ggcagcctgg aacagcggga
    29881 gtatggcaat actctgcagg cgggcggggt attccgcagg gagtacacca tcaccgtaga
    29941 ataaatccag aaacagcagc gatatgttag gtatcgctgc tgtttttaag tggctcatct
    30001 tatgaattat tcttgttctt gtaatggaaa ttcggaatat gctcccatct gtcccggaaa
    30061 taatgggcga tcagtttcgg acggcggtct cgggtaaaga tgcctttccg gttgccgtct
    30121 gcgcgcatac agccctgcag ggtggcataa tcggcgaagt tccatgcctg ttccccgata
    30181 aaaaagtctc ttccgtcgaa ggccttgctg tacattttat aaagttctac ctgaaattct
    30241 tcactccaca tttccggcgc acagttatgg agcccggcga gagtatcggc gccgtattcg
    30301 gtcatcatca cgggttttcc gatcttttcc cagaaatcca gttccagatt ccaggcgtag
    30361 gaagcagcct ccagatcacc ggagagattg taccatccgt aatagcggt
//
LOCUS       DAWEJW010000022        29625 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_654_length_29625_cov_64.524112, whole genome shotgun sequence.
ACCESSION   DAWEJW010000022 DAWEJW010000000
VERSION     DAWEJW010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 29625)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29625)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29625
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_654_length_29625_cov_64.524112"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1463..1562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8388 a         6162 c         8101 g         6874 t
ORIGIN      
        1 acagcggaac cgacagcaga acctacaaca gagcccacag cagaacctac aaaagaaccg
       61 acagtatccc cgactgcaga gcccacagcg actccgacag tacccccgac ggcaacagca
      121 accccgacag cggcccctac ggcaaccccg acagcggccc ctacggcaac cccgacagtg
      181 gcccctacgg caactcctac agcgactcct acggcaaccc ctacagcgac tccggcggcg
      241 acaattactc tggataaaac ggcagtgacg acttatcaga aggcaacgga tacagttacc
      301 gcaaaagtca gcggaagtgg aaccgtaagt gcggcttcca gcgataccgg tatagcaact
      361 gtggctgtaa gcggcaagac cattactatc accggcgtga aagcgggaag tgctacggta
      421 acggtaacgt atacagaagg cagcaacaag gtggaagcca agtgcacagt aacagtaaag
      481 gcttccaatg caagagaaga taaaaccacc aagctgaaag acaagagcgg tgtacagctg
      541 tatgtgcagg atggtgacag ctatcgtgaa gcagtctatg cggattactt tactgcctct
      601 aagttcttta tcaaaggtga tgtgaaatac accggatggc agacactgga cggaaagctt
      661 tatttcttta cagcggatgg caacaaggtt accggagaac aggtcattca gggcgcaaaa
      721 tacaattttg ccagtgacgg atctctggta gttggttccg gcaccatggg aatcgatgtg
      781 tccaagtgga atgggaaaat tgactggaac gccgtgaaga attccggtgt cagctatgtg
      841 atcatccgtg tgggttatcg cggttcctct cagggagcac tgatcgatga ccctaccttc
      901 aagaccaaca tcaagggagc tacggcagca ggactgaaag tgggtgtata cttctttacc
      961 caggcagtgg atgaggtcga ggcagtacag gaagcctcca tggtactgga ccgtatcagc
     1021 ggttacaaga tttcctatcc ggtattcctg gatgtggaag gaagcggcgg aagaggtgat
     1081 aagatcgatt ccgctaccag aacggcagta tgtaaggcat tctgcaatac cattcagaat
     1141 gcaggataca ccgcaggtgt gtatgccaac aagacctggc tcagccagaa gatggatgca
     1201 agtgcactga gtggctacaa gatctggctg gcccagtatg cagcagcacc cacctacacc
     1261 ggacgctatg atctgtggca atacaaatcc acaggaaaag tcagcggcat cagcggcaat
     1321 gtggatctga acctcagcta cctgggatat taagatttgc aataaagccc agaccgactg
     1381 cttgcaggcg aggctgggac tttattagca aatcaaacac acatgagcat gtaggacgat
     1441 agtccggaat gcgaatgtgt gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnaggagccg tgttgatatc ctgcttgttt tatggtaaaa tatccctatc tataacaaaa
     1621 aatgtgcgct gtgcgcagaa cggagaaaaa atgagaactt atctggtaac aggaggggcc
     1681 ggttttatcg gttccaacta tattcattac atgtttaaaa aatacgataa tgaaatccgt
     1741 atcatcaacg tggatgcact gacctatgca ggcaatcttg agaatctcaa ggacgtggag
     1801 aagcgtgaga actacacctt tgtaaaggct aatatctgtg acaaggatgc catcagcaaa
     1861 attttcgcag agaatgatat cgaccgtgtg gtacattttg cggcagaaag ccatgtagac
     1921 agaagtatcc gcaacccgga gatcttcgta cagaccaatg tcctgggtac tgcagtgatg
     1981 ctgaactgtg ccaaggcagc ctgggagctt cccgatggca gcttcaaaga aggcaagaag
     2041 ttcctgcatg tatctacaga tgaggtctac ggatctcttc ccgatgatga caatgcattc
     2101 ttctatgaga ccactccgta tgatccccac agcccttact ccgcaagtaa ggcaagctcg
     2161 gacatgctgg tgaaggcata tatggatacc tatcatttcc ctgccaacat taccaactgc
     2221 tccaacaatt acggtccttt ccagttccct gagaagctga tccctctgat catcaataat
     2281 gcattgcatg gcaaaaagct tccggtctac ggtgacggta agaacgtccg tgactggctg
     2341 tatgtggaag accatgctaa ggctatcgat atggtacagg aacagggaag actgttcgaa
     2401 acctacaatg tgggcggaca taatgagaag cagaatatcg agatcatcca cattatcatt
     2461 gagactctgc aggagatgct tccggattcc gatccccgca aggcacatat taacaacgac
     2521 ctgatcactt acgtggagga tcgtaaggga catgacagaa gatatgccat tgcaccggac
     2581 aagatcaagg cagagatcgg ctggtatccc gagaccatgt tcaaggaagg catccgcaag
     2641 accatcgcct ggtacttcga gcatgaagac tggatgaata atgtgaccag cggtgattac
     2701 cagaagtact acaatgagat gtacgaagaa aagtaaagaa tacctgttaa atgggaacga
     2761 tgaacgtcgt tcccattcgg tgtgttaaaa aacaagtaaa aagaagcccg aaaagggcgg
     2821 atcggaggac gaaatgaaag gaattatact ggcgggaggt tccggaacca gactgtatcc
     2881 cctgacaaaa gcgatttcca agcagatcat gccggtgtac gataaaccca tgatctatta
     2941 tcccctgtca acactgatgc tggcagatat cagagaggta ttgatcatct ccaccccaag
     3001 agaccttccg gtatttcagg aattgctggg agacggcagc cagctgggca tgcgcctgga
     3061 atatgcggtg caggatcagc cccgcgggct ggcagatgcc tttatcatcg gtgcggattt
     3121 catcggtaat gacagagtgg cactggtact gggagataat attttctacg gacagagctt
     3181 ttccgcagta ttgcgcagag tagccgccag agaaaagggc gcaactattt tcggctatta
     3241 tgtgcgtgat cccagagaat acggtgtagt ggaatttgat gagaacggca aggcactttc
     3301 catcgaggag aagccggagc atcccaagag caattatgcg gttcccggac tgtatttcta
     3361 tgacaatgct gtagtggaga tcgcaaagaa tgtaaagccc tctgcaagag gagagattga
     3421 gatcaccagc atcaacaacg agtacctgaa cagaggcagt ttacaggtag agacactggg
     3481 aagaggcttt gcatggctgg ataccggcaa tcatgatgcc cttctggatg cggcagattt
     3541 tgtggcggct ttccagaaga gacagggtct gtacatttcc tgtattgaag agatcgcttt
     3601 taagcgagga ttcattgaca aagagcagct gcttgctctg gcagagccgc tgcttaagac
     3661 gaactacgga aaatatctgg tagaggtagc aaatggactc taagctgcgg aaaatggcga
     3721 ttctggcctc catggcggtg atcctgctgg tggcactgct ggtcatgtat gtcaacaggg
     3781 agcagtttac ggcagcttcc ggaccgccgg catccggcac tcagaatgcc ggagcgggtg
     3841 acagtgcatc tccggcggaa aatggcgatc aggcagaaga gacggtctca ccggatggtc
     3901 agatcggcaa tgatctgaag gcatttttaa aagataatac gttttttgat caggaagtaa
     3961 atccaattct ggaagctgca aaggataaca gcaaccgtct atctctggta gccacctcca
     4021 tagaaaagga tctgcggatc cagatcgtgg acaacgaagg aagtccggtg accggggaga
     4081 gcttctacgt acggctggat ggcctgggtg attacaagga tctggatcag gacggtatca
     4141 tttacattgg agatctggat tccggagact attatattga gctccttccc attgagggat
     4201 ataaggttcc ggtcagtgag acaagagttc atgtcaagga taaagtggag tttctggcca
     4261 ttgacgatat ttccctgttg atcaagacgg aggatgaagt ggatgcagaa gccgaggaca
     4321 gcgccgtggc aggtgctctg gcagatgcgg acaagacaga gatccagaag ctgcagacca
     4381 cgtccggtaa cgcaaaggtc ggtatcgatg tctccaaatg gaacaaggag atcgactggg
     4441 ataaggtgaa aaatgcagga gtgcagtttg ccattatccg tgccggatac cgcggttcag
     4501 taaccggaag cctggtggaa gatcccatgt tcgtgacgaa catgaagggt gcacaggccg
     4561 caggaattcc ggtgggcgta tactttttca cccaggctgt ggatgagaag gaagctgtgg
     4621 aagaggcttc cgcggtgatc gaactgatcc gggattaccg gctgaactat ccggtattta
     4681 tcgataccga aggtgcgggc ggtaacggcc gtgcggacag tctggatgct gagaccagaa
     4741 cactggtatg tgaagcgttc tgcaggacca tagaaaatgc cggatacacg gcgggtgtct
     4801 atgccagccg taactggtac aacaataatc tgcagacagc aagactggaa aactatcata
     4861 tctggctggc agagtatcgc agtgttccgt tatatcaggg atattataag acctggcagt
     4921 atacctccaa gggaaaagtg gacggcatcg aaggtcgtgt ggacatgaat atcacctacg
     4981 aatagtcaat aaaaataaag agtgaggacg aaataaatgg gtaaaataac agtagaaacc
     5041 tgtgaaattg aaggattaaa gatcattact cctacggtat tcggagatgc cagaggatat
     5101 tttatggaga cttacaatta taacgatttc aaggctgcgg ggattcctga ggtgttcgta
     5161 caggacaatc agtccgcttc caagaagggt gtattgcgtg gactgcattt ccagaaacat
     5221 tttcctcagg acaagctggt gcgcgtgatc cgcggagaag tctatgacgt ggcagtggat
     5281 ctgcgggaag gttccaggac tttcggccag tggtacggag tgcttttgtc tgaagagaac
     5341 aagaagcagt tctttatccc gaagaatttt gcacatggtt ttctggtact gtcggattat
     5401 gcggaattct gctataaatg tacggatttt tatcatcccg atgatgaggg cggtattgct
     5461 tacaataccc cggatatcgg tgtaaaatgg ccgattcccg aaggtatgga actgacgctt
     5521 tccgagaagg ataccaagtg gggcacactg gaacagtacg tgaaggaaaa agagaatgaa
     5581 ggtaagtaag aaaacgggta ttgccctgtt tgtgacagcg gcagtgatca tggcggtgct
     5641 cattgtattc cataagaatc cgggacctgc ggtagatccg ggacaggaac tggccaagaa
     5701 gatcatttcc tgtgtggtga ttgcagcggc atgctttgct tttattcact ggtatgataa
     5761 attcaccgga ctgccggtgg agctgttcca gaaccggcat ctgatctgga aactggccaa
     5821 aaatgatttc aaaaaaagat atgcgggatc ttatctgggt gcggtatggg ctatggcaca
     5881 gccggtggtc acggtggcta tgtattacgt tgtttttgat aagatcatgg gtaatacgag
     5941 tactcccctg cgggagggtg tggaagtacc ctttgtattg ttcctgacgg cgggactggt
     6001 accttggttc tatttcagtg aggcattgaa caatggaacc aatgcgctgc tggaatataa
     6061 ctatctggtg aaaaaagtag tatttaagat cagtatcctg cctatcatca agattattgc
     6121 agccaccttt attcatgtat tttttgtgtg tctgctgttg attgtggcag caatttacgg
     6181 gtattatcct accatatata cgatacagat cgtatactat agcttctgcc tgttcatttt
     6241 tgtactggca ctcagctata cgacttgtgc agtagtagta tttttccggg atctgtctca
     6301 gatcatcagt atcggattgc agatcggcat gtgggccact cctattttgt ggaatctgga
     6361 ttccctgtcc ggcaattgga tcacgattct gaagctgaat cctctggtgt atattgtaaa
     6421 cggttatcga agtgctattt atgagaagga atggtttttc caggatttct tttccaccat
     6481 gtatttctgg atcgtaacgg tagtactgtt cgggttggga gccgttattt tcaaacgatt
     6541 gaaggtacat ttcgcagacg tgctataata cgaatgattg ttacgatttc gagagttcga
     6601 agaacggaga aatcgtatta taaaaaacag agcatagcaa aacttttata gatgaagaac
     6661 cttgtgggga ggagctgcat tacaaaactg ttgtggggaa aacagaaagg gtgaaagtaa
     6721 ttgaagggtg ctcgtgaaaa atttagaaaa ttttggttat ggaagccggg aaagatcctg
     6781 ctggccgtgg gatgctgcgg actgctgctg ttgacgctga ttccattgtt tcgtctgaca
     6841 gtctatgcgg ttccgtatta tgatgattac aatttcggaa gatttgccag aactgccatg
     6901 gagcaggaac agtcaaaatg ggctgccatc tccggtgcac tggattgctc cagaacgcag
     6961 tggtatgcgt ggcagggaac ctattcttct attttcttta tggcattgat gccggcaatc
     7021 tggggagaac agtattattt cctcggtcca gtgttcatcc tgctgctatt gctggcgggt
     7081 accatgacat ttacccatgt gatcctgcgg aaagtactgg gcatggaaaa gtggaccggc
     7141 ctggcagcac aggcagtgat tacagtggca gtctttatgt ttatttattc cgcacagagt
     7201 ggcttctact ggtataacgg cggagttcat tacgtgggaa tgcacggctt tggattgttg
     7261 atgttgtctg tggcaatctg cctggagaga gcagaaggaa gaactgctac cgggctgcta
     7321 tttacggcct cggtactgct ttccatgatc acggccggaa gcaattttgt cacggcattg
     7381 cagggactct tatcgctgtt gacgattctg tttataagtg tggtagtgga acaccggaaa
     7441 aagggactgt ggctgttgcc tgctacgctg gtatacatta taggatttgg gctgaatgta
     7501 gcggcaccgg gcaattccgt gcgggcccgc agctatgtcg gctggggata cagtcccctg
     7561 gagtccatcg gcagatcttt tctggaagga gtaaaacatc ttccagagtt tacgggatct
     7621 attgttctta tggtaatggt gatgctgtta cccctgatct ggcaggcact taaggagacg
     7681 gagtatcgtt tccggtatcc gggagtcgta cttctgtggt ccttctgcct gtatgccacc
     7741 ggttacaccc caagcctgta ttccctcgga catgccggat tgtccagaac tctgaatgcg
     7801 gtgaagatca catatttatt gctgttgttc ctgaacgaaa tatactggtg tggctggctg
     7861 cagcaaagac tgcagaagaa ggcatcaggc agcacggcaa gatggctgac gaactggaac
     7921 ggagccgcag tgtggtggtt ttatgcgatt atgggtctgg aatttatcat gatctttcaa
     7981 gtgtcaccca atcaggcagg acattattcg gcttatgggg cctattacta tgtgcatacc
     8041 ggagaagcgt ataatttcca tcaggaatat ctggaacggg tggagaagct attgggagat
     8101 gaggatgatg tacagctgca accttatcac tacaagccct ggtttttgtg catgggagac
     8161 ctctcggagg atgaaaataa cgaggccaac cgttctctgg ccatatggta tgacaaagac
     8221 agtgtaacgc tgataagcga ggattaaaat tgatgcagga aaagaaaaag ctggccattg
     8281 aagtcgataa tgtccagaag atctataaac tatatgataa gccctcagat cggatgaagg
     8341 aggctttcgg cttcggaaaa aagaagaagc ataagcttca ctatgccctg aagggggtgg
     8401 acatgaagat ctatcaggga gaaacggtgg gtattatcgg taccaacggt tccggtaaat
     8461 ccacgatcct gaagatcatc accggtgtat tgaatcccac ttcgggaaga gtgctggtaa
     8521 atggccgtat ttccgctctg ctggagctgg gtgccggatt caatatggaa tataacggca
     8581 ttgaaaatgt gtatctgaac gggacgatga tgggattttc cgagaaggag atcgaggcaa
     8641 agcttccgga aatcttaagt tttgcggata tcggagatta cgtctatcag ccggtgaaga
     8701 cttattccag cggtatgttc gtccggctgg cctttgcggt ggccatcaat atcgagccgg
     8761 agatcctgat cgtggatgag gctctgagtg tcggtgacgt atttttccag gcaaaatgtt
     8821 atcataaatt cgaagaattc aagaagatgg gcaagaccat tgtgttcgtc agtcatgatc
     8881 tgagcagtat cagcaaatat tgtgaccgtg tatatctgct gaatcaggga aatatcctgg
     8941 gcgaaggcag tcccaaggct atgatcgaca cctacaagag ggtattggtg ggacagtatg
     9001 acggcccgga gagtgtggga gaagagactg ccagtctgct ggatgatgaa gacctgcaga
     9061 gagcagctgc ccaaaaagca gatgagaagg ggaccgatgc ttcccaggct tccatggagg
     9121 ccaaggggca gaatcccaat gccctggaat acggtacgaa acaggcacag attgaggaat
     9181 tttacattac ggatgacaga ggggttccca ccaatgcgat cctgaagggc agtatgttta
     9241 ccatccatat gcgggttcgt tttatggatc atattccggc ccccattttt gcattttctg
     9301 taaaaaacgt catcggtgtg gagatcaccg gtacaaatac catgattgaa aaggcttttc
     9361 tggacagcgt ggagccggga gaggtgaaaa atgtgacttt tacccagaag atgaacttac
     9421 agggtggtga gtatctgctg tctctgggag tgaccggcta caatcaggat accttcgagg
     9481 tatatcacag actgtatgat gccttgaata ttacggtggt atcggacaag aacactgtag
     9541 gatattatga tatggattcc gaaacaaaag tatgggatgc ggaggagaag taaggaatga
     9601 cagagaccat cgggaagatc acactgaatc tggataaata tcccggcgag gattattatt
     9661 gcgacggcag tgtggaggac gagatcttag acattgtaaa aaaatacagt acggtggaat
     9721 atgacaggat cattgcggag cgaaagagct ggccgatcct gtatcatttg tctgctctcc
     9781 gggaaaatat cgtggatttc cttcccatac agaaaacaga gaaagtgctg gaagtgggca
     9841 gcggatgcgg agccatcacg ggtgcactgg caagaaaagc cggggaagtg acctgtgtgg
     9901 atctctcgaa gaagcgcagc ctgatcaacg cctaccgcca cagcgaatgt gaaaatgtga
     9961 cgatccatgt gggcaatttc acggatgtgg aaccggaact tcccgcagac tatgactata
    10021 tctgtctgat cggtgttttt gagtacggac aggcttacat aggcggcaag acgccttacg
    10081 aggatttcct taagatcctg cagaagcatc tggcaccgga cggcaggatc gtgattgcca
    10141 ttgagaacaa atacggattg aaatattttg ccggatgcaa ggaagatcat ctgggcagct
    10201 ggttttccgg cattgaaaat tatccggagg gcggtgtggt acgcacgttt tcccggaaaa
    10261 agctggagcg cattttcgat gcctgcggtg tgggagaacg cagtttttat tatccgtatc
    10321 ccgattataa atttatgacc acagtctatt ccgatgctta tcttccagga cgtggagagc
    10381 tgtccaacaa tctgcggaat tttgaccggg acagaatgct gttgttcgat gagaagagtg
    10441 cttttgacgg aattgtggag gaaggattgt tttccgtttt ttccaattcc tatatggcgg
    10501 tgatcggagc acctctggat ctgaaatatg ccagatattc caatgaccgt gcggaaagct
    10561 tccggatccg caccgagatc ctgcgggaca aggaaggctg taagacggtc cgaaaatatc
    10621 cgctgacaaa agaagcggaa gcccatgtgc gtcatatgcc ggaggcttat gaaaaattga
    10681 aggaacggta tgtgggaagc agtctggatg tcaatgtctg ccatctggga gaagagaacg
    10741 gcatacctta tgcggaattt gagttcgtgc cgggcagacc gctgtcggaa ctgatggacg
    10801 aatgcctgga cagacaggat gtggaggaat ttcacaacct gttcgcagaa tatctggaga
    10861 gagtgggcta cggggaggat gtgccggtgg cggactttga tctgatcttt gccaatattc
    10921 tggtggatgg tgaccactgg acactgattg attatgaatg gacttttgac cgtcccatag
    10981 agaccagagc tctggccttc cgggctgtat actgttatgt actggaagat gaaagacgta
    11041 atgcactgga actggatcgt attctggatc gtctgggcat cacggaaaac gaagccagac
    11101 agtacagaga gcaggagatg gaatttcaga aatatgtgac cggacagaaa ttgtccatgg
    11161 gtgagatccg caatctctta ggcggcgaaa tttataagcc cacggaatgg atcggacggt
    11221 tccgtcagac ggagggagaa ctcagggtac agatctatga ggacaaggga cagggatttt
    11281 ccgaagaaaa ttcctatttc ccggagaacg tctatgcaga ggaaaaacag gcggaattta
    11341 cggtgaattt cgatggaaat gtccattatt tacggctgga tccggccatg tgcgcctgcg
    11401 tgtgtaaaat ccgggagctt accatgaacg gacagccggt tcctgtgcag gacaaaaaga
    11461 tcgttaccac caatggaaaa atcttaaagt ccgcagacgg tgcggagcat cccagcgtgg
    11521 tatttcccac ggaggatccg aatctgacga tccgtgtgga tgcattggac agaaaggcag
    11581 agaacatcct gactgtgaaa atggagatcg tgcagattcc ccttgctgtg gcaagtgaca
    11641 tggtgggagc ggtgaaaaaa tttttctaag gagacaggaa acttgagcat tcggacgtca
    11701 gtaaagcaga tgcttgtcag acaacaggat aaaaagtatg aagcagaact ggcaaagctg
    11761 cgggtgacct acgcgcaatg ggctgcggag caggaaaaaa agattgcgga aacagtggtc
    11821 acagagatcg gggaaagagc cggtcttgcg gaattcgtga tctacaggca gcagaaagga
    11881 cagctggcgg aaaatgctgt ggagcggatc aatgcgtatt ttgtgaagca tccggaggca
    11941 gagatcgtct atggagatga agatctgctg tcagagaacg gagagcgtgt gatcccctgg
    12001 tttaagcctt gctgggctcc ggatacctac cgggctttct tctatgtggg aagcgtcgtg
    12061 gcagtgcgga gcagactgtt gcagaaactg ggggagcccg gggctgtgac agaaggagag
    12121 agcaccggca gggaaatagt attttcgaaa gcggaagaga tccgtcccct gatggaccgg
    12181 ctgtttctgg cggccggtgg atttgagaga ggctgtcata ccattggaca tctggaagaa
    12241 gtgctgtttc acggcacttt tggtactgcg ggaatcggac tgcagggacc ggcagaaacc
    12301 agccgggaga aagcggaaga tgagcagaac ccctgggaag agtaccggac ggcagcggaa
    12361 tccgcaaaac tgtccgtgga actggcggcc aaagcggcag aggaagccag agaactgttc
    12421 gccagagagc ttagggtctc tgtgatcatt ccttccaaag ataatccttc cgtactggga
    12481 aaatgtctgc ggtcgctgac gcaacgcccg gagggcagtg ttccggtgga aatcctgctg
    12541 atagataatg gaagtaacga ggaaaacagg aaaaaaacag aacagctggt ggaagaaatc
    12601 cggacagccg gaacaccgat ccggtatgta tatgagcctg cggagttcaa tttttccacc
    12661 atgtgtaatc gtggcgcaga actggcagat ggcaagctgc tgctgttctt aaatgatgat
    12721 attgaattat gtgagaatga ctggctggat aaaatggtgt ccagagcctt gcagccttat
    12781 gtgggcagtg tgggactgaa actttattat ccggattccg tgaagatcca gcatgacggc
    12841 attgtcaatc tgccggtggg accggtgcat aagctacagt ttatggagga tgacagaagc
    12901 tactattttg gacggaaccg ttttacacag gattgtgtgg ccgtgacggg agcctgtctc
    12961 ctgatccgga cagaggtgtt ccgggaggcc ggaggattcc gggaagtgtt gcgggtagcc
    13021 tataatgatg tggaactggg cttccggctt ctggaaatgg gatactacaa tgtggtgtgg
    13081 aacgaccgct ttgcatatca ccatgaatct ctcagccggg gaagcgatga aagtcctgag
    13141 aaaatgcagc gtctggtaca ggagagagaa ctgctctacc agatgcatcc acagttccgg
    13201 ggagaggatc ctttttaccc gaaggggctg aaccgggagg gactggacag ccgtgtggtt
    13261 ccggcatatc tgacagatcg gaatgtttta caggagcctt tctggaaaag gggacttcct
    13321 ggcggagagg aattacagaa gatccgcagg gataactgcc tgatggccag agtagagaca
    13381 gcgggaccgg agcggatcca gggatacagt gtaattctgg gagacgataa tgcctgctat
    13441 gaaaaacatc tgttgctgat tccctgtggg gaaacagaag gacaggatgt gtggtccatg
    13501 cagttgatgc ctgcctaccg acaggaactg gaggaaaatc ttccggatca gaaaaatgtg
    13561 gcattgggcg gcttttgtgt cctcagagaa ggagaacaac tgccggcggg aaactatacg
    13621 atcgctgttc ttgtggtgaa tcgtgtcagc aagctgaaac tttggaatac tacgggaaaa
    13681 tatctgaccg tagagcttcc ggcagcaaaa gaataaaaca gatgcatgat ccgctaaaat
    13741 gcggtgaaag catcacggag gagacatatg tcagagagca acaccgagat caattataag
    13801 gaagcctacg agagagagca tgaaaaatgt gcggatctgg cggacaagat cgtagtactg
    13861 gaatcggaga aggaagatct gcagttcaaa ctggaccgaa tcaagaaaaa taaattctgg
    13921 aaggctacag gacctgccag aagtgtgatc cattttgccc agagacagaa ggaacggctg
    13981 tctcattgcg gtggtcccaa agatattctg cggaagatcc gtaacaagag tcaccagaag
    14041 agcctgatgg aatcctacgg taccggaagc tttcccgatg cggagcagag gaagaaagag
    14101 gaagaaacgg tattccccta tatgccgaag atcagtattc tcgtgccgtt gtggaataac
    14161 aagagagagt tccagataca gatgctggac tgcgtcatga accagaccta tcagaactgg
    14221 gaactgtgtc tggcggacgg ttccgatgcg gaacatgctt acatcgaaga actctgcaag
    14281 gagtacgcgg cgaaatcaga tggccgtatt gtatataagc acctggacca taacggaggt
    14341 atttcctaca acaccaatga gtgttacaag ctggccaccg gagactatat cggactgttc
    14401 gatcaggatg acattctgca tccttccgta ttgtatgcct atgtcaagga aatcaatgaa
    14461 cagggtgcgg attatctgta ctgtgatgag accaccttcc aggaggacaa tattaataaa
    14521 atgctgacca tgcattttaa gccggattat gcaccggata atctgcgggc caacaattat
    14581 atctgtcatt tcagcgtctt ttccagaaaa cttctggaag gcgaggaact gttccgtacg
    14641 gcttttgacg gtgcgcagga ccacgatatg atcctgcgtc tgacggacag agcacaaaag
    14701 atcgtacatg tgcccagact gatgtattac tggagaagcc atgcgggcag taccgccgcc
    14761 agtattgacg caaagcccta tgccatcgag gcggcaaagg gtgccgtggc ggatcatctg
    14821 aaaaagcatg gattcaaaca ttttcagatc accagtacca gagcctgtgc cacaattttc
    14881 cggatccgct atcagattct gggagatccg aagatctcca tcgtgatcgc caacaaggat
    14941 cacgtggaag atctgaagag atgtattact tctattcaga agaacagcac ctggagcaac
    15001 tacgagatca tcgtggtgga gaacaacagc accacaccgg aaatccggga ttactattcc
    15061 cagctgctgg gattgtccgg caatgattcc tacgcagaga gatgcaagct gcataccgtc
    15121 tgcggtcatg acggcgggat tctgcacagc gaggacggcc ggatctctgt ggtgacctat
    15181 catggtgact ttaattattc tgctgtaaat aatctgggag tatcctatgc caccggagat
    15241 tatatccttt tgctgaacaa tgatacggaa gtgatcactg ccaactggat ggaagagatg
    15301 ctgatgtatg cacagcggga agatgtgggt gccgtaggtg caaagctgta ttatgcggac
    15361 aagacgatcc agcatgccgg tgtagtcatc ggactggggg cacatcgcac cgcaggacat
    15421 acccattatc gtattccggt acagaacctc ggctacatgg gacgcctgtg ttatacccag
    15481 aatgccacgg cagtgaccgg agcatgtctg ttggtgaaaa agagtctgta tgagcaggtg
    15541 ggaggactgg acgagagctt tgtgatctct ctgaatgacg tggatttctg cctgaaactg
    15601 cgcaaactgg gactgctgaa tgtatggacc ccttttgcgg aactgtatca ttatgaatct
    15661 atttccagag gtctggatga tcagggagag aaggcggagc gttacaacaa agaatccgag
    15721 catttccgtg aaaaatggaa ggcagagctg gaagcaggag acccctatta caacccgaat
    15781 ttctctctgg atcgcagtga ctacgcgctg agagatccgg ttagcggacg ttaaattgct
    15841 cttttcagta aaggtaatct atggtaaaat aatggcggag agcgtttact gaagaaagat
    15901 aaagatagcg ataaaacagg aggataggaa aatgagttat atggacgaac tgaatttctg
    15961 gttgaatgat gattattttg accagaagac caaggatgaa cttctggcaa tccgtaacaa
    16021 cgaggcagaa gtggaagatc gtttttacaa ggaactggaa ttcggtaccg gcggtttacg
    16081 tggcgtgatc ggtgcaggaa ccaaccgcat gaacgtgtat accgttcgta aggcaactca
    16141 gggtcttgct aattatatca ttaagcgcgg cacccagagc aagggtgtgg ctatcgcatt
    16201 tgactcccgc cgcatgtctc ccgagttttc cgatgaggca gcaagatgtc tggcagccaa
    16261 tggcatcaag gcatacattt ttgaatcttt aagacctacc ccggaactgt cctttgccct
    16321 tcgtacgctg ggatgtaccg caggtatcgt agtgaccgca agccacaacc ctcctgagta
    16381 caacggctac aaagtatact gggaggacgg tgcacaggta accgctccta tcgataagga
    16441 catcatcacc gaagtacagg ccatcaagga ttatcatacc gtgaagacca tgagcagaga
    16501 agatgcggaa aaagcaggcc tctatcaggt gatcggtaaa gagatcgatg acaagtacat
    16561 ggctgagctg aagaagcaga tcattcatcc cgatgttatt caggaaatgg ctgatgacat
    16621 caagatcgta tatactccgt tatgcggtac cggtaacaag ccggtaagac gtatcctgtc
    16681 cgagctggga tttaagcacg tctatgtagt tcccgaacag gagaatccgg atccaaactt
    16741 caccacactg gattacccga accctgagga tcccaaggca tttacatacg cactgcgtct
    16801 ggccaaggag aaggatgcgg acatcgttct ggcaaccgat cccgatgcag accgtctggg
    16861 tgtgtatgcc aaggatacca agaccggcga gtatgtggca tttaccggta acatgtccgg
    16921 tatgctgatc gcagaatata tcctgagaga gaagactgct accggcgcta tgcccgagaa
    16981 tcccgcactg gtaaccacca tcgttaccac caatatgacc aagcccatca ccgaagctta
    17041 tggtgtaaag ctgatcgaag tactgaccgg cttcaagttc atcggtgagc agatcaagtt
    17101 cttcgagcag accggaagca acaattatgt attcggtctg gaagagagct acggctgtct
    17161 ggcaggtact catgcaagag ataaggatgc agttgtagca gttatgtgtc tgtgtgaagt
    17221 ggcggcttac tgcaagaagc acaacatgac tctgtgggat atgatggtat ccatgtatga
    17281 gaaatacggt tacttcaagg agacccagta caccattacc atgaagggta tcgacggtgc
    17341 aagacagatc gccgagatca tggataagct tcgtaagaat ccgcccaagg cattcggtga
    17401 cctgaaagtg gagaaattcc gtgactatct gaacgatgta gtcatcgaca tggagagcgg
    17461 cgagaagacc actaccggac ttcccaagtc taatgtcctg tattttgagc ttcccgacaa
    17521 tgcatggtgc tgcgcccgtc cttccggaac cgagcccaag atcaagttct acatgggtgt
    17581 taaaggtaac agcatagagg atgcacaggc caaggtagag aagctgaccg aggatgtaaa
    17641 ggctgtactg taaccaccat gagcaaatgg catgcaagta tgttattcag gaaagctctg
    17701 gaagagcgat tttgcgaatg gggttctgaa tagttaatgt tatttttaag agcgaatcgc
    17761 ccgggaaggg cgatttgctc ttttataaaa gtggaggtat ccgtggcaaa gctgaacata
    17821 gcaaacctta agaaaactct atattatttg caacgcaacg gcctgaggga aacctggatc
    17881 tctgtaaggg aacgcctgac agagacagac cgctattttt atgtgccctg tccggaagag
    17941 gaactggaaa ggcaaagttg cagaaaatgg gataatccgg ttacgctcag cattgtggtg
    18001 ccgttgtacc gtaccccgga aatctatctg aacagaatga taacaagcgt tatgcaacag
    18061 agctatcctc actgggaact gattctggcg gatgccacag aggatcacag tgtgaaggag
    18121 acgttgacaa atcagggatt cctgacagaa cggctcctgg aaaatgcgga aactattgca
    18181 gcggatgcaa ggatccacta tatacacctt acggaaaatg ccggaattgc ggcgaatact
    18241 aatcaggcat taccctatgc aaggggagaa tatatcggcc tgctggatca tgatgatgta
    18301 ttgacaccgg atgctctgta tgagatggca gatgctatta ccaaagcaaa tgacagaggt
    18361 gtcagagttg cattcgctta ttcggatgaa gacaaatgca acggggatga gacaaaatat
    18421 tacgatccca accataaaga agactttaat tacgatttaa tcctgagtaa taactatatc
    18481 tgccattttc tggtgatgga tgcagatctg atgaagaaac tggccttccg gccagaatgc
    18541 gacggtgccc aggactatga tctggtgctg cgggcagtgt cagaggtgct ggctgcagat
    18601 ggccgctccg gggaggagag aattcttcat attcccagag tgctgtatca ctggagatgc
    18661 catgaagcat ctacagcagc caatccccac agcaagaaat atgcttacga agcggggctg
    18721 cgtgcactgc aggatcatgc ggcagaacgt gggatcccgg caaaggcaga ggagaccaga
    18781 catgtaggat tttatcggtt acaatataca gaggtattac aggagagacc ggatgtggct
    18841 gccgtaggtg gcagagtgct gtctgggaaa aaccgcggca ggatcgccgg cggcagaatg
    18901 actgcggacg gaaaggtgtt ctacgaggga ctgccgaagg actttggcgg ttatcttcac
    18961 cgggcggagc tgtcccagga tgcagaagcg ttagaccttc gctgcatcag gatccggtca
    19021 gctgacaggg aattgtttga aaaaatcgtg ggagtgcctt atacggaagt agtaagaggt
    19081 tcggaacaac agccggtatt tgacagctcc acactgcctg ccggggcaga tatccggctt
    19141 ttaagtctgc aattatcgga agcattacga aaacggggaa gactgctgta tctgccggag
    19201 tatccggaga aatgggagag actatgaagg aagtaaccgt cgttataccc aattacaacg
    19261 gaatgaaata tttaccggaa tgcatggctt ccctgtgtca gggacaggaa aatgcaccgg
    19321 aatatgaagt gctgatcata gacaatggtt ctgctgacgg gagcgtggaa tatctggaca
    19381 gcgaatggtg tcgcaaaaaa gaggcaccgg cagaagaggg gggaaaaccg caggtgcgtc
    19441 tgattgccct gccggagaac accggcttct gtcatgcggt gaatctggga atccgggagg
    19501 cacgaagctc ctatgtgatc ctgttgaaca acgataccag agtagaagct ggatttgtga
    19561 aaggactgta tgatgccatt cggaaggatg aaaagatctt ttccgtcagc gccaaaatgc
    19621 ttatgtggga taagccagag ctgctggatg atgcgggaga ccgctactgc gttctgggat
    19681 gggcatattc ccggggaaaa ggccggcctg ctgtggatta tgacaagaat gtcagcatat
    19741 tctcagcctg tggcggagcg gcaatttaca gacgtcaggt atttgaggag atcggctatt
    19801 ttgacgaagc gcattttgct tatctggaag atctggatat cggttaccgt gcccggatct
    19861 acggctatga gaaccggtac gagccgacgg catccgttct gcattacggc agtgcttcta
    19921 cgggatcccg ctacaattcc cggaaaacgg aactttcttc ttccaataat atctatgtca
    19981 tcggcaaaaa tatgccgcta ctacagtgga tcctgaatct gccgttcttt ttgcttggat
    20041 ttggcataaa atttctgttt ttttgtaaga agagaatggg aaaactatat ttaaagggat
    20101 tggccgaagg gtggaaacgt accgtgggtc ctgtgggaaa gcaggcaaag gtgcactttc
    20161 aatggagcca tctatggaat tatgtgaaga ttcagtggca attatacctg aatgtttttc
    20221 gaattcttat gaaatcttaa gaaattgctt cataaattct taagttgaga aaaaaccacc
    20281 gttattgtag aatgaaatcg ttgaaaagaa gcttttcgga cttaaagatt gggtgacccg
    20341 tatgataaaa gacaaccaga aagtctttaa caggcttctt gtgataatag atgcgctgat
    20401 cacggcagca tcttttgtcc ttgcctatta tatcaaattt tatatcttaa atgacggtcc
    20461 ggggatcggt gtcctgccgg tgcaggatta ttatatgctg cttcccttta ttgtacccgg
    20521 ttatatgttc ctgtattacc ggtgcagtgt gtattccccg aagcgtacgg ttcgccgtag
    20581 acatgagata tacagtatcc tgcaggcgaa taccattggc ctggcggctt tgatcattat
    20641 tttgtatatg atcatccgtg agatcaattt ttcacgatcc gtcatggcaa ttttttatgt
    20701 cctgaatgtt tttctgactt ccgtattccg tatcatcatg cgcaaggcat tgcgttccat
    20761 acggaaaaaa ggatacaatc tgaagcatat tttgctggta ggttattccc gggcggcgga
    20821 ggaatatatt gacagaatcc tcagcaatcc ccaatgggga tatgtagtgt gtggtattct
    20881 ggatgagcac atcccgggcg gcacgaccta taagggagtg aaagtactgg gaacgcttgg
    20941 taatctggaa tacatccttc cggagaacaa gctggatgaa atcgcaatca cactttccct
    21001 gaaggattat gattatctgg aaggtgtggt ggatatctgt gagaaatccg gggtgcacac
    21061 caaattcatt ccggattaca gcagtctgat ccccagcaga ccctatacgg aggatctgat
    21121 gggattaccg gtgatcaata tccggtatgt accgctgacg aacacaggca atatggtgat
    21181 caagcgggca atggatatcg ttggttccat tttcggtatc attattacct caccgatcat
    21241 gctcatatca gcaattctgg taaagctgtc cagcccggga ccggtgatct ttaagcagga
    21301 acgggtagga cttcataata agccgtttta tatgtacaaa ttccgcagca tggcaatgca
    21361 gacggcggcg gaagagaaaa agggatggac ggtacgcaat gaccccagag tcaccggtat
    21421 cggtaaggtt ctcagacgta ccagtatcga tgaacttccc cagctgttca atatcttaaa
    21481 aggagatatg agtctggtcg gtcccagacc ggaaagaccg cagtttgtgg aaaagttcaa
    21541 ggaagagatt ccccgttaca tggtaaaaca tcaggtacgg ccgggactga ccggatgggc
    21601 acaggtgaat ggcttgcgcg gtgattcctc catcaagaag agaatcgaat atgatattta
    21661 ctatattgag aactggacca ttggatttga tatcaaaatc attcttatga cgttctttac
    21721 aggatttatc aataaaaatg cttattagta caaggagaag acaagatggc aacaaattct
    21781 aacaacaatg gcggcaatgg tcagagacct gccggaagcg gaagaaaacc ttccggaagc
    21841 gcacagagac ctacaggaca gagacccgcc ggaagcggtc agagacctgc cggcagtgga
    21901 cagagaaccg gagctgcgaa cggacagaag agaatgtctg ccaaacagcg tgcagccaag
    21961 aagagaaaaa aggttatcat ctttggcata gagatcgtag tgatcctgtt catggtagca
    22021 ttattgtatc tggtaatgac tcataccaac aatgagggcc ccaaggtaac cgttctggag
    22081 acagaagaac ttcagattcc cgaagaggtg cagcaggatg aaactatgaa gggctatatg
    22141 aacattgccc tgttcggtgt ggacgccacc aaggaaagcc tgttgtacaa gggcagccga
    22201 agcgatagta tcatgatcgc cagcatcaac atggataccg gtgatatcaa actggtcagc
    22261 gtatatcgtg atacgtacct gaataccggt acggattcct accagaaatg caatgccgct
    22321 tattcctatg gcggagcaga gcaggcggtt aagatgctga acatgaatct ggatatggat
    22381 attacgaact ttgtgaccgt aggctataaa ggactgagcg aagtgatcga cggtctgggc
    22441 ggtatctata ttgatgtgga ctctgaggag atcaagcata tcaacaacta tcagatcggt
    22501 attgcagaag ttttgaaatg cgattataca ccggtaactg agaccggtat gcagctgttg
    22561 aatggtctgc aggctactgc ttactgccgt atccgttata ccgctggtaa cgactttaag
    22621 cgtgctgccc gtcagcgtga agtcattcag gcaatcgaag atcaggcaaa gaaagcagat
    22681 tatgcaacat tgtccaaggt attcaacagt gccatcgacg acatttacac cagtctggat
    22741 tccaaggaca tcctggatct gctgggcaat atttcaaatt atcgcatagt ggacgaagga
    22801 ggtttccccg aagagaccat gcgtgacacc ggcaatatcg gtgcaaaggg aagctgtgtt
    22861 attccggtaa atctcgaaag caatgtagta tggctgcatc agttcctgtt tgatgatgcc
    22921 aactattccg taaccagcaa cgtgaaggaa tacagcaata agattgaatc tgacacatca
    22981 ccttatatga ataagtaaga aacagtgtat agagtaaagc aacaggaata caagggagtc
    23041 ttccggaatc tgccggggct cccttgtgca ttaccaagaa ggttataatc ccttggacag
    23101 taggaaaagg cttgtgccgc aataagagtt aaagtataat gaaccagaga ttggaaaagt
    23161 gaggagcgct ctgacatgga gatagatgtg atcattcccc tgtataagcc gggaaaagaa
    23221 ttatttacat tattggataa actgggcagt cagagcgtac cggtacatca ggtgattctt
    23281 ctgaatacgg aagaaaaata ttttgaacag ctgatctacg gaatcagatt tctggaaacc
    23341 tatcagaatg tcaaggtata ccatgtatcc aaaagagagt tcgatcatgg cggtacgaga
    23401 cggatgggag taaaaaagtc ttctgcggat gtcttcgtga tgatgacaca ggatgccatg
    23461 ccgaaagatg acaggctgat cgaaaaactg gtggaacccc tgcaggggga agtggcggtg
    23521 gcctatgcga gacagctgcc cagagaggat tcgacaccgg tggagagcta taccagagaa
    23581 tttaactatc cggcaaagtc ccggatcaaa tcggcagcag acctggacag cctggggatc
    23641 aagacctttt tctgctccaa tgtctgtgcg gcctatcgcc gggagatcta tgaggaactg
    23701 ggcgggttcg tcagacatac gatatttaat gaagatatga tctatgcggc aaaggcggtg
    23761 gaagcaggat actctgtggc ttatgcggcg gatgcgcagg tggtgcattc tcataattat
    23821 accaacgggc agcagtttca cagaaacttt gacctgggag tatcgcaggc agagcatccc
    23881 gagatttttg cagcatatcc ttcggagtcc gagggaaaac gaatggtgaa agatgtgacg
    23941 gtatatttgc gcagcaacca tatgtctgcc ggattgcccc atttttatat gcaatgcgcc
    24001 tgtaaatatg caggttattt actgggaaag aactatcgca ggctcccgaa aaaatgggta
    24061 cttgctttta cttccaacaa ggaatactgg aaacagaaca gaaaggatgt atgactatgt
    24121 gtggtattgt aggttatatt ggaacagaac aggcagcacc gatcattctg gacggtctgt
    24181 ccaagttgga ataccgtgga tatgattccg caggtatggc gatttttgac ggtgagaaga
    24241 tcaacaccag aaaagcagtg ggaagactta aggttctgga gaacctgacc cacggcggag
    24301 agaatatgaa aggaacctcc ggtatcggac atacccgttg ggcaactcac ggagctccca
    24361 gcgatatcaa ttctcatccg catatgaaca atgcgggaac cattgccgtg gtacacaacg
    24421 gcattatcga gaactatatt cccctgaaga aaaagatgca ggataagggg tatcagttcg
    24481 tatccgagac tgataccgag gtattggcac atctgttaga ctattattac aagggaaatc
    24541 ctctggaagc catcacgaag gtgcttcatc gtgtggaagg ctcttatgcg ctggggatca
    24601 tgtttgcaga tcagccggat aagatttttg cagcccgcaa ggacagcccg ctgatcgtag
    24661 gaaagagcga caacggcagc tttatcgctt ccgatgttcc tgcgatattg aaatatacca
    24721 gaaccgtata ttatgtagac aatcaggaag ttgtggaatt acagcagggt agtctgcgtt
    24781 tcttctccgt ggatgaggaa gagatcgaga agcagccggt gaccattgac tgggatgcca
    24841 atgcggcaga aaaggctgga tatgagcact tcatgctgaa agagatctat gagcagccga
    24901 agaccatcaa cgataccatc tctccgagaa tcaaggataa tgatattgtg atcgaagagc
    24961 tgaacatgag tgatgaagag atcaaagaga tcacgaagat ccatattgtg gcctgcggct
    25021 ccgcatatca taccggagtt accgcaaaat atgtgatcga gggtctggca agaatcccgg
    25081 tagaagtgga tgtggccagt gaattccgtt atcgtgatcc gattcttcag aagggtaata
    25141 tggtcattgt catcagccag tccggtgaga cggcagatac tctggcagca ctgagagagt
    25201 ccagggaaag gggcttcaag gtactgggca ttgtcaatgt ggtgggaagc tccattgcca
    25261 gagaggcaga cagtgttatg tatacctggg caggtcccga gatcgcagtg gctaccacca
    25321 aggcttacag tgcacagcta actgcacttt acatgctggc tatgaaattc gccagggtac
    25381 gtggtactat tgatcaggag aagttcgccg gtatgctgac agatttaaga tgcctgccgg
    25441 atcagattga actgcttctg ggacagaagg agaagatcca gcattttgcc aaccgttacg
    25501 tgggagcaag agatatcttc ttcatcggac gcggtattga ttacgcgatt tcactggaag
    25561 gatccttgaa actgaaggag atctcctata ttcattccga ggcttatgca gctggagaac
    25621 tgaagcatgg tacgatttct ctgatcgaag atggtacgct ggtagtggct ttggcaaccc
    25681 agccggatct gttccagaag accatcagta atatggtaga agtaaaagca agaggagcct
    25741 ttgttatggc agtcaccatt gaaggcaaca aggccattga gaaagcgtcg gattatgtaa
    25801 tatacatccc cgagaccaat ccgtacttta ccaattctct ggcaatcatt cctttgcagt
    25861 tgttctccta ctatgtggca gtgggacgcg gctgtgacgt ggataaaccc agaaacctgg
    25921 caaaatccgt tactgttgag tagagtgtaa taacttaccc atagagagac tgcttgcagg
    25981 cagaacagac tctctgtttt ttcaggattc ataattgctt cagaaagtta tcacaattcg
    26041 gacacaattg acggatatac tgtgttcata aggtgagagg aagaaatgaa acaggtaatg
    26101 gtaaaaagag tttctcactt gcaacaggat aggaggaagc aatatgtacg agaatgataa
    26161 tttttccggt tacggaaccg gcaatgaaaa tacaaccggt acattcagtg gagcagatct
    26221 gaatgagaca agaacagaaa gcagttatgg ctatgaaaat catagtacta cccagagtgc
    26281 agcaggaagc acgaatggtt acaccactta tcagatgggc ggaaccacag gcagcaacaa
    26341 tacggagcct aagaaaaccc aaaaacatgg cggttacttc cggaaggcaa tggtaagtgt
    26401 cagcctggga ttgttcttcg gactgttcgc aggaatcggt ttctatgcag tacagcaggg
    26461 aaccgggatg tttacgacca gcacagatac cgcagtggta gatgagatca cttctgaggc
    26521 aacgacagaa agtacccaga gtgcacagct ggctaccaat gttacttatg tagagtccga
    26581 tgtatccaat gtggtagaaa aagtaatgcc tgccatggta tccatcgtaa ataactttac
    26641 cgagacagcc aatgtcttcg gacagcagta tacacaggaa gaagcagcca gcggttcagg
    26701 tatcattgta ggtaagaccg atgatgaact gctgatcgta tccaataatc atgtagtaga
    26761 gagtgcagat actctgaccg ttacttttat cgatggcagt gaagcacagg cacaggtcaa
    26821 aggactggat tccgatatgg atctggcagt gatcgcagta tctttaaatg atctgagtga
    26881 tgataccaag aatgccatta cagtagcaac acttggcagc agtgatgatc tgaagcttgg
    26941 tgagccggtc atcgctattg gtaatgccct tggctatggt cagtctgtta ccaacggtat
    27001 tgtaagtgcc ctgaatcgtg agatcactct ggagaacggc tctaccggtc tggagaacgg
    27061 ctctaccggt actttcatcc agaccaacgc agccatcaac cccggtaact ccggcggtgc
    27121 actgctgaac atgaatggtg aggttatcgg tatcaactcc aacaagatcg gtggtactgc
    27181 cgtagaaggt atgggatatg ctatcccgat tacttcggca agtcctatca ttgcagatct
    27241 gatggagcgt cagaccagaa ccaaggtagc tgaggatgaa gtcggttaca tcggcatctc
    27301 cctgcgggaa gtaacttccc agatctccca gatgtacaac atgccggaag gtatctatgt
    27361 agtatctgta gaggaaggca gcgcagcagc caatgccggt atcatgaaag gtgatatcat
    27421 taccaaattt gacggcagca gtatctcttc ttactctgat ctgcagaaaa cgttacagta
    27481 ttatgcggtc ggtgattccg taaccgtaac ggtacagaga cctcagaacg gtgaatatgt
    27541 atccatcgag ctgaatctga cactgggcag cagacctgca agccagaaat aaagaaataa
    27601 caggtaaata aaaggaaatg gaaagagacc cgggcgagac atatggtttt gcccgggtct
    27661 tgtaatattt accaaaatat gaatagttat aaatggttta gaaaaactta acttgtgccc
    27721 cgctacttca ttgagtataa taagcgtagg caatgagaag tgtcagacac gggtattatg
    27781 caatatgact gcttgcaggc attattgcgt aatggccggg gatgataggg cgaagcccgt
    27841 atatcgaggg aacacgaagt gttgccgaga tacactgcga agatgctttg gagccgggga
    27901 tgatagggcg aagcccggat atcgagggaa cacgaagtgt taccgagata cactgcggaa
    27961 tagtggtagg aaacaaagag gagaataaca tgacagataa ttataatgat gattatagag
    28021 aagtgatccc taaggaggaa aaaacagaga ccgatgtgag tgaaactcag gataacgaaa
    28081 aaaataacgg aaacacacag gtggcggata ctgcaaaaga aaattccacc gaaaaaaagt
    28141 cagaggacaa aaaggacagc agcgaatacg aagatgtctg ctttatctgc cgcagaccgg
    28201 agagcaaagc cggcagaatg ttcaagctgc ccaaccatat ctgcatctgc gatgattgca
    28261 tgcacaagac catggagact gtgagccagt tcgattatca gggaatgttg aacaatccca
    28321 atgatttcaa ccagtttaac ggacagaacg gattccccaa tatcagtttt gtgaatctgg
    28381 cggatcttca gggagacggc ggtattccca acaagcagaa actcaagaag aaaaagaagg
    28441 cggaaggtcc caaacctgta ttcaatatca aggacattcc ggcacctcat aagatcaagg
    28501 ccagtctgga tgaatacgtg gtaggacagg aaaaagccaa gaaggtcatc tccgtagcag
    28561 tatacaacca ttacaagaga gtggccactg acactatgga tgagattgag atcgaaaagt
    28621 ccaacatcct catgatcggc cccaccggtt ccggtaagac ctatctggta aagacactgg
    28681 caaaacttct ggatgtgcct ctggctattg ctgatgccac ctctctgact gaggccggtt
    28741 atatcggtga tgatatcgag agcgtggtat ccaaattgct ggcagcggcg ggcaatgacg
    28801 tagaaaaggc ggaaaaaggt atcatcttca tcgatgagat cgacaagatc gccaagaaga
    28861 aaaacaccaa ctcccgggat gtcagcggag agagtgtaca gcagggtatg ttgaagcttc
    28921 tggaaggtgc ggaagtggag gtgccggtag gtgccaacag caaaaatgcc atggtacctc
    28981 tggcaacggt gaacacccgc aatatcctgt ttatctgcgg tggtgcgttc ccggatctgg
    29041 acgagatcat caaggagcgc ctgaccaaga ccgcatccat cggtttcaac tcagaactga
    29101 aggataaata cgataaagat accgatattt tatccaaggt gactgtggag gatatccgca
    29161 agtttggtat gattccggaa tttatcggtc gtctgccgat catttgtact ttacagggat
    29221 tgtcaaagga tatgctggtg aagatcctga aagagccgaa gaatgcgatt cttaagcagt
    29281 accagaagct gttggagctg gatgaggtaa aactggagtt cacagacgat gccctggatg
    29341 ccattgccga gaaggcaatg aagcgcgata ccggtgccag agctctgcgc tccatcatcg
    29401 aggacttcat gctggatatc atgtatgaga tcccgaagga tgataacatc ggaagggtta
    29461 ccattaccag agagtacatt gaaggaaccg gcggtcccat catcgacatc cgcagtaatg
    29521 agatcctgga acccgtggag aatctgaagc agattccggt ggaagagaaa taaattgaga
    29581 gttagtgaaa tacattggca aaatccacga actcccggag catgc
//
LOCUS       DAWEJW010000023        29371 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_665_length_29371_cov_60.019887, whole genome shotgun sequence.
ACCESSION   DAWEJW010000023 DAWEJW010000000
VERSION     DAWEJW010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 29371)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29371)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29371
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_665_length_29371_cov_60.019887"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10016..10115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8202 a         6422 c         8204 g         6443 t
ORIGIN      
        1 aaatttttca aaaacagagt gacgaatgaa agcagcggct tcctcgggag aggcgtagtt
       61 cgccgcaatg ggcgtatagt ccatataaca ttcatcgatg ctgacctgtt cgatgtcggg
      121 gcagataccc gccagatgtt ccataagcat ctggcttttc agacggtaca tattatgatc
      181 cggagaagcc atgaccagat gcggacattt gcgcagggca ttgaccaccg gttcaccggt
      241 agtgatgccg aaagccttgg cgggaatgga tttggccagc accacaccgt gtctggtgga
      301 ggtatcaccg cctacgatgg aagggatcgt gcgcagatcc acaggatccc cctgttccag
      361 acgggccagc gccgtccagc ttaaaaaagc ggaattgaca tcaatatgaa aaatgaccgg
      421 ttccctcatg gcgtctcctt tccggatcag accgttttat ttacctccag tatagcatag
      481 atcggttcgg gagaaaacaa aaaaatcgaa cagacattct ttctttacaa ggcatataaa
      541 tgctgataga atctatatgt tacaaagtta ttacagtaag acaggtatac agaatgcccg
      601 aagggggcat actgttttca gatacgatag atcgagaagg aattcatgaa gaaactgaca
      661 tcttactcga aaagagtaaa atatacatat gtgaaaacaa cgctggccac tctttttgtg
      721 agcctctttt tcctcaaagg gtatgcagcg tttgaaaaga ccggagagaa ttacttccac
      781 gtgttcctga acggcaatga gatcggaacg gtgggcgaag cagacagagc ggagcagctt
      841 ctgcaggaag ccagaaggaa tatcgcttcc cagagtgatg atatgatctt tatggacgca
      901 gatttaaagc tggtcggtga agaagtgctg tggggaacac tggacaatga ggacaatctg
      961 ctggcagcca tggaacggga actggagagc agtattcgtg agacttccca cagatcctat
     1021 acaatgaaag taaatgaata tatggtcaac ctgtccagcg tggaggatgt ggagtctctg
     1081 ctgcaggcag cagtggacaa atacgacagt gaagacaaat tcagcgtgga actgactcac
     1141 gatgcacaga aggaattcaa tgttcttacc accgaggtag tcaataaaca ggaactgacc
     1201 gctcaggaag aacagaagga agaatccatc tatgcaggcg gtgtacagtc cgtactggat
     1261 caggcgggct cccaggcaga ccagcagggg gaaaaagatt tcgaagacta tgatcttggg
     1321 ctgatgacca tggactttgc ggaaaaggtg gaaatcgtag agacctatct tccggaaagc
     1381 atgctgactc cgctggatca ggcaatcgag gatgtgatca aggatcagga gacccccgga
     1441 gaatatgaag tagtcagcgg agataccctg tcggagattt cgattaaggt aaatattccg
     1501 atggatcaga tcatcgccat gaacagcgag acactgaccg atgaaaattc tacgatccgc
     1561 gtaggggaca agctgatcat taccgtgccg cagccggagc tgtccgtgga gcgcatggag
     1621 caaaattatt atgaagaaat ctatgatgca gatgtaattt atgtggacaa cgattcctgg
     1681 tatacgaccc agaccaagat tttgcagcag ccctccgctg gtttccgtcg ggtagtggcg
     1741 gatgtatctt atctgaatga cagagaggtc accagggata ttctgaagga agaagtggtc
     1801 atggaggcag ttcccaagat tgtggaacga ggcaccaaga ttcctccgac ctatatcaag
     1861 cctatttccg gtggacgtct gagttccaat ttcggacgca gaaagtcacc taccaagggt
     1921 gccagcagct accataaggg tgtggactgg tcggttccta ccggaactgc ggtatttgca
     1981 tcctgcggcg gtacagtggc cagagccgga tggggaagtg gatacggtta ttgtgtctat
     2041 atcaaccatg aagacggcag acagacccga tacggacatc tgagcaaggt gctcgtcaag
     2101 gtcggccaga cggtaaaaca gggagaacgt attgcactga gcggtaatac cggcgtaagt
     2161 acgggacctc atcttcactt tgagatcctc attaatggca gccaggtgaa cccgttgaag
     2221 tacctgaatt agggcgttgt atttacaaat gtgcgaaaat gtggtataag tgcataagaa
     2281 ggtttttctg atctgagaat gacagaggta agaaacaatg gaacaatacg caattaaagg
     2341 cggtaatcca ttagtcggtg aagttgaaat aggaggtgcc aagaatgcgg cacttcctat
     2401 tcttgctgct tccgtgatga cggatgagac tgtttatata gaaaatatgc cggatgtaag
     2461 agatacgaat gtccttttga gcgccatgga gaacatcggc gtgattgtaa agagaaccga
     2521 ccgtcacaaa gtgactctca atgcatccaa catcagtgaa cttgtagtag aagatgaaaa
     2581 tatcaagaag atcagagcgt cctactatct gatcggtgct ttgttaggta aatacaagca
     2641 tgcggaagtg gcacttcccg gtggatgtga tatcggctgc cgtgccatcg accagcatat
     2701 taagggcttc cgtgctctgg gcgcggacgt gaagatcgag catggcctga tcaagacaca
     2761 ggcggagcat ctgcgcggca gccatattta tatggatgtg gtcagtgtcg gcgctaccat
     2821 caatatcatg atggctgcgg taatggcgga aggcaccacg attatcgaga actctgccaa
     2881 ggagcctcat gtggtggatc tggcgaactt cctgaacagc atgggagcca atatcaaggg
     2941 tgccggtacg gatgtgatcc gtatcaaggg tgtttcccat ctgcatggca cagagtatgc
     3001 cgtgattccc gatcagatcg aagccggtac gtttatgttc gcagcggcag tgaccaaggg
     3061 cgatgtgacg gtgaaaaatg tgattccgaa gcacctggag tctattacgg caaaactcct
     3121 ggagattggc tgtgaagtag aggaagcgga tgactgtatc cgtgtggtag cgtccaagcc
     3181 tctgcagcat acccaggtga agaccctgcc ttatcccgga tttcctacgg atatgcagcc
     3241 ccagatcaca gtggcgctgg gactttccaa aggaaccagc atcgtgacgg agagtatttt
     3301 cgagaaccgt ttcaaatatg tggatgagct gacccgtatg ggcgccagca tcaaagtaga
     3361 aggcaacacc gctatcatcg acggtgtatc cagatatacc ggagccaata tttccgcacc
     3421 ggatctgcgg gcgggagcag cactggtact ggctgccatg tctgcagaag gattctctac
     3481 tgtggatgat atccgctata ttgagagagg ttatgaggat ttccacctga aattacaggg
     3541 actgggagcc cagatcgagt tggtcgagac ggagcgggac ctgcagaagt tcaagctgaa
     3601 gatcggttaa tgagtggatt tctcataaac cacaacagaa tgacattttg aaaaaaatgt
     3661 ctgttgacaa aacaggtaac tcatagtaag ttaattagcg tagaaaagtt catatagact
     3721 ttcccttatt cagagtggtg gagatacata ggatcgatga agccacggca accccgcaga
     3781 atgcggaagg tgcccacctg agcgaataac tgtcgaacaa taagaggatt tgatcgcaca
     3841 ataaatcagt ccgcttatga aagcggactt ttttgcatca ttcagagctg tcggcattga
     3901 aaatttgatt ttgcaaatga ggctctgaat gacaggactt tagcaactca tacactagac
     3961 cctcttcccg caaggaagca gaatggagga caaatggaaa aacgtttatt tacatcggag
     4021 tccgtaaccg aaggacatcc cgacaaaatc tgtgatgccg tatctgatgc ggtactggat
     4081 gcgctgatgg agcaggatcc gttcagccgt gtagcatgtg agacctgcac caataccggt
     4141 ttcgtactgg taatgggtga gatcaccacc aaggcaaata ttgatattcc cgctattgtt
     4201 cgtaagaccg tgaccgagat cggctatgat tcttccgaaa agggattcga cggcaacacc
     4261 tgtgccgtta tggtatctct ggacaagcag tccactgata tcgccatggg cgtagacaag
     4321 gccctggaag caaaagaaag ccacatgacc gatgaacagc tggataccat cggtgccgga
     4381 gatcagggta tgatgttcgg ctatgctacg gatgagacac cggaatatat gccgtatccc
     4441 atctctctgg cacacaagct gaccagacag ctgaccaagg ttcgtaagga tggtacactt
     4501 tcctatttaa gacccgacgg aaagagtcag gtatccgtag agtatgatga gaatggcaag
     4561 ccttttcgtc tggaggcagt cgttctgtct acccagcatg acgaacatgt atctcaggag
     4621 cagatccatg aggacatcaa gaagtatgta ttcgatcctg ttttgcccgc agacatgatc
     4681 gatgcagaca ccaagttctt catcaatcct accggacgtt tcgtaatcgg cggacctaac
     4741 ggcgacagcg gactgaccgg ccgtaagatc atcgtagaca cctacggtgg atatgcacgt
     4801 cacggcggcg gagcattctc cggcaaggac tgcaccaagg tggacagaag tgcggcatac
     4861 gcagcacgtt acgttgccaa gaatatcgta gcggcaggac tggctgaaaa gtgtgagatc
     4921 cagctgtctt acgctatcgg tgtggcacat cctacttcca tcatggtaga taccttcggt
     4981 acgggcaaac tgcatgatga taagctggtg gagatcattc gtgagaactt cgacttaaga
     5041 cccgcaggca tcatcaagat gctggatctg cgcagaccta tctacaagca gaccgcttcc
     5101 tatggccact ttggacgtac cgatgtggat ctgccctggg agaagctgga caaggtggat
     5161 ttattgaaga aatacctggc atagttacga gccatgcaaa aataaaacag gaactgttcc
     5221 cgggagttta ctcacaggga acagttcctg ttttgatttt tatgcggcac atgtatgaat
     5281 gaagcatggc gttttatttg gtttcctctg tctcctcggt ctcgtctgtc tcttcctgtc
     5341 catactctcc gatatggaac agctgctgtt ctccggaggt agtgacatgc acaccttttt
     5401 cttccagctt cagatccttg acatacatgg tcttcgtagt ctggggcaga gcggccagat
     5461 caatttcaac gggctccagc agatgtgcgg gatctgcttt gtaatgcacc tcggacaatt
     5521 ctgcttcgaa gatcccctgt accttgtctt cgttcacata gacaatggga acagtagcgg
     5581 atacgatttc gccggctacc agagcctgaa agtccagagc catatatttc ttggtcagag
     5641 gatcaaaatc aatatttttt accagaacat ctacattctt accatccagt tccagataag
     5701 cctgggtgcc ttcttttgca ttcttgataa aagcggatgc atccttctct gtgaatttca
     5761 gggaaacatt ctctttcatt tcttttccgt acaaaacacc ggtgataaaa ccgtctcttc
     5821 ttaatttctt tgctttttcg tcaggatttc ttttttcagc ttttaatgtc aacataatga
     5881 tgacctcctc gatctctacg tttgtttcgt gtagatgaga gcttgtagaa gttctttttg
     5941 cactatggtt attatagcac atatagaaca aagtaaacag ttaaaatgtc acaaaaataa
     6001 cagaaaaaaa gtggaattta caagcaaatt tcagaaaaca atgtgaagcg gcactctttg
     6061 gcatctacga catgaaacga aaatatgtaa aacacatgga aatattcgtt tcaaaattgt
     6121 gtgcgtatga tgatatatct gagaaaaatt tacaactgca ttacaagaac acgttacaac
     6181 aataaagtaa aaggttgcgt aaaaaggtaa aatggggtat acttgtcaca aatacatttg
     6241 aatttactgg gaggacataa ggatggatat gaatattgta tgcctggatc tggagggagt
     6301 actggtgccg gagatctgga tcgcttttgc ggaggccagc ggaatcccgg aactgaagag
     6361 aaccaccaga gacgagccgg attacgataa attaatgaaa tggagacttg gtattctgaa
     6421 ggaacacggt ctgggactga aggagattca ggagactatc gcaaagatcg accccatccc
     6481 cggggcgaag gagtttctgg atgagctgcg ttccattacc caggtcatca ttatcagcga
     6541 cactttttca cagtttgcgg gccctctgat gaaaaaactg ggttacccca ccatcttctg
     6601 taattccctg gaggtagctg acaacggtga aatcaccggt tttaagatgc gtgtggagaa
     6661 ttccaagtac actacggtga aggcattgca gtctatcggc ttccagacca tcgccagcgg
     6721 tgacagccac aacgatctgg gcatgatcca ggccagcaag gcagggttcc tgttcaagag
     6781 taccgaacag atcaagaagg atcatccgga gctgccggcc tatgagacct atgacgaact
     6841 gatggcagcc atcaaaaaag cattataatc tcctatatta ctattactat gaaaaactct
     6901 ccggacagat gttattctgt tgaggagagt tttctgtgta tacagtgaat gcgtttaatg
     6961 gagaatattg atccgccaca cacggaaaac agggaaaaga caaataaaat ataaaaatat
     7021 aatcgaaatt aaaaaagtta tttacatatt tttacaaaat gttacaatat tataatatga
     7081 cttgttatca accaatagta aggagaggaa aatgttcgaa aatttgaaaa tcaggcagcg
     7141 cctgaaaaaa gcttttgcca ttattgtggt tatatcatct attgcaggag tgctcggagc
     7201 actggcaatg atcgttacca tgaaccgcta tcagcatgca ttgaccaatt acggtttttc
     7261 acagggagat atcggtaagg ccatgactgt attcacggat gtaagatccg caacccgcgg
     7321 aatcatagga tataacgacc cggatctggt ggacggtctg atcacgactc acgatgagaa
     7381 gaagcaggct tttcttgaat actgggaagt tgtcggcgag actgttgttt cttcaaagga
     7441 gaaggaactg tatgatcagg taaatgagca gattcagcag tactgggagc tggatcagca
     7501 ggccattaat accggtaagg tggatatgga agagaagcgt atgcaggcac aggagctgat
     7561 gcagaatcag gtagcaccgc tttataatga aatttatgat accatgcgtg aacttatgaa
     7621 cgagaatgtc cgtgaaggag attccctttc ttccatactg aatatcatgg gatttatctt
     7681 cctgcttatc attgttggtg tgatcgtgct ggcaattatc attgcaacga gaatggaaca
     7741 tgcaatctct cagggaatcg cagcaccgct ggatgcattg gcaaagcgtc tggagacttt
     7801 tgcacagggt aacctgtccg atccgttccc gacactgaac agtaaggatg agatcgctga
     7861 catgattcat tctgcaaatg aaatggcaga aaagctgagt tttgtcattg ccgacaccgg
     7921 agaagttatg agccagatgg caaacggcaa ttacaacatc acttccaaga acccggatat
     7981 gtatcagggt gattttgaac agttgtttct gtctatccgt aacatgaaga cacagatggt
     8041 tcagacactg cgttccatcg aggagtcttc caggcaggta tccgcaggtg cagctaacct
     8101 ggcagatgct tcccagaatc tggcagaggg cgctaccgag caggcaggtg ccgtagaaga
     8161 actgcaggct accattacca acattacttc caatattgag caggctgctg aacaggcaca
     8221 ggagtcctat gagcaggcca gaaaatatgc aaacgaagca gataacagca gagcagagat
     8281 gaaggctatg gtggatgcta tggccagaat cagtgatact tccaacaaga tcggcaatat
     8341 catttctgaa atcgaggata ttgcatccca gaccaacctg ttgtccctga atgcttccat
     8401 cgaggcagcc agagcaggtg aggcaggccg tggattcgct gtggttgctg accagatccg
     8461 tcagctggca gagcagagta ccaagtctgc tgtagatacc agagacctga ttgaaggttc
     8521 catgcaggag atcaaggaag gcaaccaggc tgcagacagc gctgctgctt ccatcgagat
     8581 cgtagtagac ggtatcggca agattgcaga gtcttcccgc agtatcagcg agatctccaa
     8641 ggaccaggcc aatgccatgg aacaggcaga gcagggtgtc aaccagatct ccgaggtagt
     8701 acagtccaac tccgctaccg cagaagaatc ttccgctacc agccaggaat tgtctgcaca
     8761 ggcaatctct ctggatgagc tgatcagcaa gtttattctt cctcaggaat aagaacggct
     8821 gagacattga gaaaaatata gtacagctgg aaaagcaggt atcggcaacg atgcctgctt
     8881 tttttctctg acaggaagtg gacaaggata tctgatatgc gatataatta cagggaacag
     8941 aactggaaca aaatgagtga aatgaggtaa tcatgggaat ggatcaggaa cagacacccc
     9001 acaagcgccg ggtgcgttat aaagggaaat accccagaaa atttgaggaa aaatataagg
     9061 aattacagcc cgaaaaatac ggagatacca ttgcccatgt gattcagaag ggaaacactc
     9121 ccgcaggcat gcatatctct attatggtga aggagatcct ggatttcctg aagatccagc
     9181 cgggagagac cggatttgat gcaacactgg gctacggtgg acatacgaaa gccatgttgg
     9241 agcagctgca ggggtgtggg catatctatg ccacagacgt agatccggag gagtctgcca
     9301 agacgaagaa acgactggca gaggccggtt acggagaaga gattctgacc atcagattac
     9361 agaatttctg taccatagat gagatcgcaa aagaggtcgg aggatttgat tttattctgg
     9421 cggatctggg agtatcttcc atgcagatcg ataatcccag cagaggattt tccttcaagg
     9481 tggacggtcc gctagatctc agactgaatc agcagcaagg aatcagtgca gcagagcgcc
     9541 tggataccat cagcagagaa gaactggcgg gaatgcttta cgagaattcc gacgaaccct
     9601 actgcgagga actggcgaag gccatcaccg atgagatccg taaaggacac cggatcgata
     9661 ctactacaaa attaagggaa gtcatcgaaa agacccttga ttttctcccg gagaaggaga
     9721 aaaaggatac catcaaaaag acctgtcaga gaacttttca ggccttgcgt attgatgtga
     9781 accgcgaatt tgaagtgtta tatgaattta tggaaaagct gccgggagcc ttgaaacctg
     9841 gcggaagagt agctatcctg actttccatt ccggcgaaga caagctggtg aagcaggcat
     9901 tgaaggaagg ctaccggaac ggcatttatg ccgattatgc gaaagacgtg atccgccctt
     9961 ctgcggagga atgtgtacag aacggcagag cccgttccac gaagatgcgt tgggcnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntccca tcccacaccg gcacaggaag
    10141 agacactgga gaccttgtat ttcgcggcga tgctggctcc cggctaccac agggaacagg
    10201 tgggagcaac ttacgaggaa tgcgtcagaa aagcaaaaga actgcactag aaagggaacg
    10261 acaaaatggc atttgcacat cttcatgttc atacagaata ttcgctgtta gacggctcca
    10321 acaagatcag ggagtatgta gccagggtca aggagctggg catggacagc gcggcaatca
    10381 ccgaccatgg tgttatgtac ggcgtgatcg atttttaccg ggcagccaga gaagcgggga
    10441 tcaagccaat cctgggctgc gaagtgtatg tggctccgaa ttcccgtttt gacaaggaac
    10501 tgaccggcgg ggaagaacgt tatcatcatc tggtgctgct ggcggagaac aataccggct
    10561 atgcgaatct catgaaaatc gtcagccgtg gctttacgga aggatattat tataaacccc
    10621 gtgtggacat ggaagtgctg caggagttcc acgagggaat tatcgcattg tctgcctgcc
    10681 ttgcaggtga agtgccccgg tttattctga agggaatgaa ggacgaagcg agaaaagcag
    10741 cccgcaaata cgaggcctgc ttcggcaagg gcaattattt tctggaactg caggatcacg
    10801 gtatcccgga gcagcgtacc gtgaatatgg aactgctcca gatgagcaga gaactggaca
    10861 ttccgctggt aaccaccaac gatgtgcatt acacctatgc tgaggatgcg attccccacg
    10921 atattctgct gtgtctgcag accggcaaaa agctggcgga cgaggatcgc atgcgttatg
    10981 agggcggtca gtattacgtc aagagcgaag aggaaatgaa gggactgttc ccctatgcat
    11041 gggaggccgt ggagaatacc cagaggattg cagaccgttg taatgtggag atagagtttg
    11101 gcgtgacgaa gctgccgaaa tacgatgtgc cggaaggtta tgattcctgg agctatctga
    11161 acaagctttg taatgacgga ctggcagagc gttacggtga cggtgaccag cctgccgggg
    11221 agaccggaca gaccctgcgg gaacgtctgg attacgagct gggtgtcatc cgcagaatgg
    11281 gatatgtgga ctatttcctg atcgtatggg atttcatcaa ctatgcgaaa gagcacggga
    11341 ttccggtggg accgggacga ggttccgctg cgggaagtat cgtggcatat tgcctgaaga
    11401 tcaccaacat tgatcccatt cattataatc tgctgtttga acgtttcctg aatccggaac
    11461 gtgtgtccat gcccgatatt gatgtggact tctgcttcga acggagacag gaagtcatcg
    11521 attatgtggg cagaaaatac ggcaacgaca aggtggtgca gatcgttacg ttcggtaccc
    11581 tggcagccaa gggtgtcatc cgtgatgtgg gacgtgttat ggatctgccc tatgcgttcg
    11641 tggattccat tgccaagatg gtgcccaatg aactgaacat caccctgagc aaagcgctgg
    11701 aaatgaaccc ggaattccga aagctttatc aggaggatga gcaggtgcat catctgatcg
    11761 atatgtgtaa gcgtctggaa ggactgccca gacatacctc catgcatgcg gcgggcgtgg
    11821 tcatctgtca gaagtcggcg gatgaattcg tgccgctgtc cagaggctcc gacggttcca
    11881 tcgtcaccca gtttaccatg acgacattgg aagaactggg actgctgaaa atggatttcc
    11941 tggggctgcg tacactgact gtgatccatg atgcggtaaa atttattgag aataccaccg
    12001 gcagacacat agatgtggat gccattgact ataacgatcc gaaagtactg gcttccatcg
    12061 gcaccggcag aacggaaggt gtgttccagc tggaaagtgc cgggatgaag agcttcatga
    12121 aggaattgcg tccccagaat ctggaagatg tggtggctgg tatttccctg tatcgtccgg
    12181 gtcctatgga ttttatcccg aagtatattc agggtaaaaa tcatccggat gcggtaactt
    12241 atgcctgtcc ccagctggag ccgatcttaa agcccaccta cggctgtatt gtatatcagg
    12301 aacaggtaat gcagatcgtt cgtgatctgg cgggctatac actgggacga agcgatctgg
    12361 tgcgccgtgc catgagtaag aagaaacagt ccgtcatgga aaaagagcgg gccaacttca
    12421 tctacggcaa cccggaggaa aatgtccccg gctgtattgc caacggcatt gacgagcaga
    12481 cagccggaca gatctacgat atgatgatgg atttcgccaa atatgcgttc aacaagtccc
    12541 atgcggcatg ttatgcagtg gtggcttacc agacggctta tttgaaatat tattatcccg
    12601 tggagttcat ggcggcgctg atgacctccg tcatcgataa cccgaaaaag gtatccgaat
    12661 atattttgaa ttgccggaac atggatattg ccattctgcc tccggatgtg aatgcgggag
    12721 aagccggatt ctccgtatcg gatggcaaga tccgttatgc cctgacggcc atcaagagtg
    12781 tgggacgtcc catcattgac agcctggtac aggagcgtaa ggaaagaggc cctttcacga
    12841 atctgaagga ctttattacc cgcatgtccg ataaaaaaga gatgaataag cgggctattg
    12901 agaacctgat caaagcagga gctctggatg gtctgggcgg tacgagaaaa cagttcatga
    12961 gtgtctatgt gcagattgcg gaccatattg cccatgataa aaagaacaat ctggccggac
    13021 agatatccct gtttgacatt gcaggggaag aggataagga agaattcgat atccggatgc
    13081 cggatgtggg cgaatacaat aaggagatgc tgctgggctt cgaaaaagag gtgctgggtg
    13141 tctacgtcag cggtcatccc ctggaggaat atcaggagct gtggcagaac tgcatcagca
    13201 atactgccgg ggattttgca ctggatgaag agaccggaga ggtgcagtta gtcagggacc
    13261 aggccaatgc agtcatcgga ggattgatcg cggacaagac agtaaaatac accaagaatg
    13321 acaagatcat ggcatttttg aatgtggagg atctgatcgg taatgtggaa gtcgtggtat
    13381 ttccacaggt gtacgagcgc tacagtacct tgcttgtgga ggatgcgaag gtgtttatcc
    13441 ggggcagagt atccctggaa gaagacaagg acggcaagct gatctgcgac cagatcatca
    13501 gtttcgagga tgcgcaggct gcgagagcgg cgaaccagcc cctgttcccc agaaattacg
    13561 gaggccgggg cggcagcagc cgcagcggat cctacggaaa cggaagtgga aatggcggtg
    13621 ccggtagata ccggaatgcc gggaacggct acggacaata ccaaaatgac ggtatgaaca
    13681 gcagtagtat gtcggtggga cagaccggtc aggcagcagg acaaatgcag tccgggcagc
    13741 gtacacaggc aaatggaacc cctcagaaaa aaggcatgcc ggcgggagcc tggatccagt
    13801 tccccaatat ggaggcctat aagaccagag agcaggaact gctgcaggcc attgcggatt
    13861 ccgacgggga tgacgatgtg gtgatctatc tgcgggacat taaaaatatt aagatcctgc
    13921 cccaaaatct ccgggtaaaa gccgacgggg cattaatgga aaagcttaca ttgctgtttg
    13981 gaaaagaaaa tgttaaattt ataacgaaac ctattgaaaa ccgctcaaaa atagattaaa
    14041 ataaggaatc gagaagagaa aactattcgt tcgtttactt aggaggtaac agtcatggca
    14101 aaagagatta agacgatcgg cgtattgacc agcggcggtg atgcaccggg aatgaacgct
    14161 gctattcgtg cagttgttcg tacagccatt gcaagaggac tgaaggttaa gggaatcaaa
    14221 aaaggctatc agggtctttt gaatgaagag atcatagata tggaagccaa ggacgtatcc
    14281 gatatcattc agcgcggtgg tacgatctta ggaaccgcac gttgtctgga gttcaaggaa
    14341 gcggaaggac agaagaaagg tgctgagatc tgcagaaagc acggtatcga cggtatcgtt
    14401 gtcatcggcg gtgacggttc ttaccgtggt gcacaggcac tgtccagact tggtattaat
    14461 accatcggtc ttcccggaac catcgacctg gatattgcat gtaccgagta cacaatcggt
    14521 ttcgatactg ctgtcaacac agcaatgcag gcaatcgaca aggttaagga tacttcttcc
    14581 tctcatgaga gatgcagtat catcgaggta atgggcagaa atgccggtta tatcgctctg
    14641 tggtgcggta ttgccaacgg tgccgaggat atcctgcttc ccgagaagta cgattacaac
    14701 gaacagaata ttatcaataa tatcattacc aaccgtaagc atggtaagac ccatcatatc
    14761 atcatcaatg ccgagggaat cggacattcc acttccatgg cccggcgtat cgaggctgct
    14821 accggtattg agacccgtgc tacgatctta ggttacatgc agcgtggcgg aagccctact
    14881 gccaaggatc gttactatgc atctatcatg ggtgcttacg cagtagatat catgctggaa
    14941 ggcaagacca accgtgtcgt aggttatcag cacggtgagt tcatcgattt cgatatcgaa
    15001 gaggctttgc agatgcagaa gggcatcaat gagtaccagt tcgaggtatc tcatctgctg
    15061 tccatctaat ttaatttaac agttcagact gcgaagcagg cacttttata aataagagtg
    15121 tctgaacgga tatgaagaca aaggcgtttc ccattgcgtg gaaacgcctt tttgactata
    15181 ataaactcat ccatagaatg catcccgact gcttgcaggc aggggatcat tctatgagag
    15241 gagtaaacaa acataagtaa gtagggcgat tagcccggat tacgcatgtt tgttatggtt
    15301 tcgagagttc gcagaacgga gaaaccatat tattatgatg tttaggagcg gttagcagac
    15361 agatatgatc acaagcaaca acaatgcaaa agtaaaacag gtggtgcagt ggcagaccaa
    15421 ggcaaaggag cgccgcaagg atcacgtatt tctggcagaa ggtatcagaa tgtgtgagga
    15481 ggctccggta aaaaacgtgc gggaggtcta ccttacggaa gaactggagc aaaagatccg
    15541 gcagaatgcc cagaacggag acgcggcatt ctgggacaag ctgcagcaga ccggctacga
    15601 gaccgtctcc acggaagttt ttgccaaaat ggcggatacc cagacccccc agggcattct
    15661 gacagtgctg gaacagccgg tctatgacct gacacagctg ttggaacagc cggatccttt
    15721 atttctgatg ctggaaaatc tgcaggatcc gggcaatctg ggaaccatga tccgtaccgg
    15781 agaaggagca gggataacgg gtgttatcat gaacaaccag acggttgata tctttaatcc
    15841 caagaccatc cgtgccacca tggggtctat tttccgggta ccctttgtct acgtgccgga
    15901 tctggcactg gtgctggaac agatgcatgc aaaaggtatc tacacctatg cggcacactt
    15961 gaaaggacag gaatactacg attccttttc tttccgggag cctacggcgt tcctcatcgg
    16021 taacgaagga aacggcttat ccaaggaaat ctccgaccag gcaggacagt atctcaagat
    16081 ccccatggag ggcagggtgg aatccctgaa tgcctccatt gcagcggccc ttctgatgta
    16141 cgaggcccac agacagcgaa gcgcaagact gtaactatcc cggacccatt tgctttatag
    16201 atattatcaa atgccaggca ttcgatgcgc agccgataat atttacatgg ctctgaataa
    16261 ttatgatgtc ggggtcaaaa gcacccgaca ccataataat agaagaaaga cagaacagga
    16321 ggaaagatat catgaagatc ggatttatcg gactgggaaa tatggccact gccatgatcg
    16381 gaggaatgtt gcaaaaagga attgcagctc cggaggacat cattggttca gccagaacag
    16441 aaacaacagt agaaaaagtt aaaacaaaat ttaatattaa cacaacaacg gataacaaaa
    16501 tcgtagcggc acaggcagac attctgttcc tggcagtgaa acccatcttt ttcccggagg
    16561 tgatcgacca gatcaaagat gtcgtggcag aggataccct gatcgtcagc attgcggcag
    16621 gaagaactct ggactatctg aaagaggctt tcggcagacc ggaattgaag ctgatccgct
    16681 gcatgcccaa tactccggca ctggtactgg aaggctgcac cggtgtctgt gcggaagaaa
    16741 acgtcacgga ggaagacatg gagcaggtac tggcactgtt acgttccttc ggcattgcca
    16801 gtgtggtacc ggaacggctg atggatgtag tcgtaggtgt cagcggtagc tccccggcct
    16861 atgtgttcat gttcattgaa gccatggcag acgaggcagt agccgccgga atgcccagaa
    16921 aacaggctta tgaattcgcg tcacaatccg tccttggcag tgccaggatg gtgctggaga
    16981 ccggcagaca tcccggagaa ctgaaggaca tggtgtgctc ccccggtgga accacgatcc
    17041 aggcagtcaa ggtactggag gaaaaaggca tgcgcgccgc tgtcatggat gccatggatg
    17101 cctgcattga gaagtccaaa agcatgtagt gacagctcga ctctgttcat gagttgagcg
    17161 ccggttcatg agcaaaggta gtcacgcagt gacgaaaagc gtgataacgc aactttgcga
    17221 atggcgctag ccgagctgtc atggctaaat agtgcccaaa gtcccaaaac ttgacaaaaa
    17281 ttttgtcatc tgttatgata tctcgcgtaa caaatttaat atttttgtaa caaaccggtg
    17341 aaaatcttaa taatttcagc atatactatt cactggtgag atccacaggt atcacaccca
    17401 tcggggaatc ttcctgtggg aaaggcgtgt atcaagatga caaaaagcag aagaatgctg
    17461 gttgtatgcg taggactgct ctgttctctc ttcctgagca tgcagatgag catgaccgca
    17521 caggcgggca atacaaaaaa ttatgtaaat gatctgaacg gtggtatcgc atccatcctg
    17581 gatcccggtt ccaggaacag cactgaggtg atcaacgcta cggtgaagga actgaacctg
    17641 actttcccgg cagaggaaga gatcagttcc ggtctggtca tggcgaacgt aaaggatgcc
    17701 gtgaatgtaa gaagcgatgc cagcgaggaa gcatccaagg tcggtaaact ctacaaggac
    17761 tgtggaggta ccatcctgga gcgtaaggac ggctggacca agatccagtc cggcactctg
    17821 atcggctggg caaaggacga atatctcctg ttcggagatg acgccagagc actggccaac
    17881 gatgtaggca ggatgatcgc acagatcaat acggagaccc tccgtgtgag aacggaagcg
    17941 gatcaggaag ccggtgtcct gggacttctt cccaagggag acatcgcaga tgtagtagac
    18001 aacagcaacc cggaatgggt ctgtattgac tacgaaggcg cagacggtta tgtggctgcg
    18061 gaatatgtaa ctgtagattt ccagatcgac tccggtgaga cactggagga gatcaaggca
    18121 agagaagccg cagagcgtga agcaaagcgt cacgtcaact acggcgaata taccaccgat
    18181 gcggatacca cgcagctgct ggccgcactg gtacagtgtg aagcaggctg tgaaagctac
    18241 gaaggccagc tggcggtagc gtccgtagtt atgaaccgtg taagaagcgg agcatatccc
    18301 aatagtattc acggtgttat ctatgcctcc ggacagttta ctccagcact gaacggtaag
    18361 gtaaataccg tatacgaatc cggcaggatc tccgcaagct gtgttcaggc agcccaggaa
    18421 gccatctccg gtgtctccaa tgtaggagac atgacccact tcagacgtaa caatggccgt
    18481 gacggactgg tcatcggtaa ccacgtcttc tactaagtgg cacacaaaag aacagcgcat
    18541 acaacagaga cggtatcccg gaacaggggt accgtctttt aacgtacagg caggagccga
    18601 tccgccagat ggaatgagga atgagaagag cacaatagaa tcctgtatcg tgcaccaata
    18661 gaggttgtca cagaagacta tttgtagtac actggataca actacaaaac caaaggagtg
    18721 atcacatgag tgaaatggta accttgtggc ttgtggtatt gatcgtatcg atcggtgtgg
    18781 aggtcgccac tctgggactg acgagcatct ggttcgccgg aggcgccgtt gtggcagtga
    18841 ttgccgccgc attgcaggca ccgatctggt tgcagatcct gctatttttt gcagtgtctc
    18901 tgctgctgtt atttttcacc agacctgtcg cagtcagata tttcaacaaa gaccgcgtaa
    18961 ggacgaatgt ggaaagtatg atcggcagac aggcaatcgt gaccagtgag atcgacaatc
    19021 tgcagggcat cggacaggta acggtgggcg gccaggagtg gagcgccaga accgaaaaag
    19081 acggactgaa tctacagccc ggaacggtgg tagacatcgt ggcagtcagt ggtgtcaagc
    19141 tgatcgtcaa ggtggatccg cagatgacga aggtcatcgg ggaagtaccg cagtcacaga
    19201 attaagaatc gtgtaacacg gatacatgag ggaaaagcgc gtacagaggt acgcagcgta
    19261 cacgaatgta cgcagaagga ggaaaaatgg tagcattaat cattattgtc gttttagtat
    19321 tatggatctg tgtatcctgt atcaagatcg tgcctcaggc acaggcctac gtgatcgaac
    19381 gtctgggtgc ttatcagtcc acctggaatg taggattcaa tctgaagatc ccgttcatcg
    19441 ataaggtagc cagaaaagtc aatttaaaag agcaggtagc ggatttccca ccccagcccg
    19501 taatcaccaa ggataacgta accatgcgga tcgataccgt agtcttttac cagatcacag
    19561 atcccaagct gttcacctac ggtgtagaga atcccatgat ggctattgag aatctgaccg
    19621 ccaccaccct gcgtaatatc atcggtgacc tggaactgga ccagaccctg acttcccgtg
    19681 agaccatcaa caccaagatg cgtgcggcac tggatatcgc caccgatccc tggggtatca
    19741 aggtcaaccg tgtggagctg aagaacatca tccctcccgc agagatccag aatgccatgg
    19801 agaagcagat gaaggcagag cgcgagagac gtgaagccgt tacccgtgca gaaggtgaga
    19861 agaaggctaa gatcctggag gcagaaggtg ccaaggagtc cgcaattctt cgtgcagaag
    19921 cagataagca ggcagccatc cttcgtgcgg aagctgagaa ggagaagatg atccgcgagg
    19981 cagaaggtga agccgaagcc atcctcaagg tacagcaggc aaatgccgac ggtatcaaga
    20041 tgctgaagga agcaggcgca gatgcagcag tcctgaagtt aaagagcctg gaagctttcg
    20101 aaaaggtagc tgacggccag gccaccacca tcctgctgcc cgcagagctg cagggcattg
    20161 ccagcgtggc agagaccttc aaggcagccg caggcaaaaa ggcagaataa gtaaactaaa
    20221 aaacgataaa ccaaagccac tttccgggaa ataaggaggg tggctttttt atgtcggatt
    20281 cacaaaaaac aatgacgggg gataaacgcg aggggttgaa aaacacagaa taatgaagta
    20341 tattgattac atatagcttg ttacaggcaa cgtttgtaac agggatatca atggacaaaa
    20401 tcagaaaaca gaggctgcgg gcgcaaggcc ggaaagcttc ggaaaagagg agtattatgg
    20461 atttaaaggg acgcgatttt ttgaaattac tggactttac accggaagag atcgaatatc
    20521 tgctggatct ggcggcagac ctgaaggata agaagaaaaa aggaatcccc gtagatactc
    20581 tccgaggcaa gaatgtagcg ctgatctttg agaagaccag tacccgtacc agatgtgctt
    20641 ttgaagtggc agcccatgat ctgggaatgg gaacgactta tctggatccc accggatccc
    20701 agatcggcaa aaaggaaagc attgccgata ctgccagagt actggcaggc atgttcgagg
    20761 gtatcgaata tcgtggcttc ggacaggata ttgtggagga actggcaaaa tattccaagg
    20821 tgcccgtatg gaacggcctg accaacgaat tccatcccac ccagatgctg gcagaccttc
    20881 tgaccatccg tgaacatttc ggacatctga agggaatccg catgacctac atgggagacg
    20941 cccgttacaa catgggtaac tctctgatgg tagcctgcgc gaagatgggc atgcacttta
    21001 ccgcatgtac caccgcaaaa tatttcccgg cgaaggagct ggtagcggag tgtgaggcca
    21061 ttgcggcaca gaccggcggc agtattaccc tgaccgagga tgtgaaggcg ggcacgaagg
    21121 atgtggacgt gatatacact gacgtgtggg tatccatggg cgagcccgac gaagtatgga
    21181 ccgagcgtat taaggagctg tctccttatc aggtgaataa agcagtcatg gacaatgctg
    21241 gcgagcaggc cattttcatg cactgtcttc ctgcattcca tgatctgaag accaagatcg
    21301 gtgcccagat gggtgaacgt ttcggcatca ccgagatgga agtgaccgat gaagtatttg
    21361 aatccggcca gtctctggtg tttgaagagg ctgagaaccg tatgcatacg attaaggcag
    21421 tgatggcggc gactttatga gtatgaaagc aaagatttta gagcttcttc gggaaaagaa
    21481 ggatcgggtg gcagcaggag caccggaaag cgaggcctat gtctccgggc aggagctctg
    21541 cgagcagttc ggtgtatcga gaactgcggt atggaaagcc atcggacaac tgaaaaaaga
    21601 aggctatgtc attgaagcgg tacagaaccg cggctaccgt ctgctggatc agacggagga
    21661 agtctacaac caggcggata tccagagcag attaaagacg aaatgggtgg cacatccttt
    21721 acagtttttt gacagtatcg attccaccaa tatccgtgcc aaactggaag cggaacaggg
    21781 cgcggagagt ggcctgttgg tggtgtcgga tatgcagagc gccggacgcg gcagaagagg
    21841 ccggggctgg gagagccccg cagggataaa tatttattac accctgatgt taaaaccgga
    21901 tttttcatcg gactgtgcac cgatgctgac gttagtcatg gcactggcgg tggcgaaggg
    21961 catccggcag acactgcgcc gcgacagcga agaggcagca gccaaagtcg ggatcaaatg
    22021 gcccaacgat attgtggtgg acgggaaaaa agtctgcggc attctcacgg agatgagcat
    22081 ggagcagggg tatatccagc acatcgtcat cggtgtgggc atcaatgtgc gtaaacagga
    22141 attcccggaa gagatccggg accgggcggc agccattgat gaacaatgcg gcttccgcat
    22201 ttcccgcagt cagctgattg cggatattat ggaagccttc gaggaagatt acgagatctt
    22261 tttacggacc catgatctga aaggcttgcg ggcatcctat gcggaacttc tggtaaatca
    22321 ggacagggaa gtctgcgtgt tggatcccaa gggcgaatac cgtggcattg ccagaggcat
    22381 caacgaccag ggagagcttc tggtggagag accggacggc accgtggagg aagtctatgc
    22441 cggagaggta tcggtccgcg gaatctatgg gtatgtgtag gcaggtacag cattttccgg
    22501 gatggcagaa ggcaggacgg acagccggat ggaaagaccg gaataaaaag accggaacag
    22561 atagtccgtg acagaattga gacagaacag gatatagaag tatggacgag aacaagatcg
    22621 tactctgcgg cgccaacgcc tatgagaaga aatattattt taacgaagaa caatttaaga
    22681 tgattccgga ttccatcaag caggagctgc atgtgatctg cgtgctcttc accgaggaag
    22741 tgggcggtgt ctttaccatt gcctttgaag aggatggaaa tgtggtcatg gagaccaatg
    22801 cggatgatga tgacatctat tatgatgaga tctcttccgg actgttgatc tctgagatcc
    22861 gtaggaacag acaggagctt ttcgagtccc tgagtctgta ttaccgtgtg tttatcaaaa
    22921 aggaagatgt atccgcattg ctggatgagg aggacgatga atgatttttg tcattgatgt
    22981 aggcaacacg aatatgacca tgggtgtctt tcaggggaaa aagctggaag ccaccttccg
    23041 gatcatgacg aagaccccca gaacctcaga tgaatacggg atcatgatca cccagctgct
    23101 aaaaaataac ggtatcgaag tgacggacat cgagggagtc atcattgcca gcgtagtgcc
    23161 ggatgtgatg cattccctga ccagttccat catccgttat ctcaagacaa aaccactgat
    23221 cgtgggcccc ggtgtgaaga gcggcatcaa ggtcgctacc gaggatcccc gggccatcgg
    23281 tgcggaccgt atcgtggatg cggtggcagc ttatgtgaaa tacggcggac cggtgctggt
    23341 actggatttc ggtacggcga ccacctatga tctgattacg gaggacggac gttttactgc
    23401 aggaatcact gctcccggta tccggatcag ttcggaggct ctgtggaaag agacggcgaa
    23461 gctgccgaat attgagatcc gtaagccgaa gaccatcctg gcacaggaga ccattaccag
    23521 tatgcaggca ggactcatgt acggtcagat cggccagacg gaatacatta tccgcaaggt
    23581 aaaggaagag agcgggcttg cggagctgaa ggtcgtggcc accggaggct taggccgtgc
    23641 cattgcggac gagaccgaca gcattgatat ctatgacagt tctctgacac tggatggact
    23701 gcggatcatc tacgacaaga acagacgttg ataggggaat gacatgataa agctggaaaa
    23761 agcagcaaca aagattatgg cagccatcgg aacagcagta atgctgttcc tgctgtttta
    23821 ttcctggaaa tatacgaaga ggctggtgga accggaatat tatctggatg aaaaagacag
    23881 tattctctgg aatgctcttg tcggcgtagt gctggtggcg gtatgcgggg ctctgtccgg
    23941 agtgatgaaa aagatcccgg cgtgggcact gcatctgctg gcagttctgg gagctgtgac
    24001 agcagtgatt ctggggataa ggcttgcgag ggccgccgga gcggagagca ttgcggacca
    24061 gtattatgtg caaagagcag ctatggcact ggcggacggt gatgcggaat gggtgctggc
    24121 acaggattac tatggtatct atccgttcca gctgggactg gcggagatct tcgccttaat
    24181 cttccgtgtg gcaggctcca cggaacggct cgtgattcaa caggtacagg cggtctgcgg
    24241 aggcatcacg ctgtatgtgg gatttaggat cgtgcgggaa ctgtcgggaa gccgggcagc
    24301 ggaactgctg tatcttgtgg cggagctttt gttcctgccg gtgtattgct atgctttgtt
    24361 tatctatggt gagagtatcg gaacctgcag cgtcctgtgt gccatctggt gttacctgat
    24421 ggccaaccgg ccggagggaa aggtgtggca gaagggactg ctgtggagtg ccgtcgcgtt
    24481 gttcatggga ctggcgtatg cgaccagaaa tgtattgctg gtggtatgga tcgccatggt
    24541 catcatgcag atcctggtgc tgcttcgcag taaaaaatgg cagggactgc cggcgctctg
    24601 cgtggtgctg gcagtcatgc tgctggggaa aacgactctc tgtcacatgg cggaaggaca
    24661 gcttgaccgg gaatatggtt ccggtgctcc gtatgttctg tgggttgcca tgggaatgca
    24721 ggagaatccc gacgccctga aggggccggg aacctacaat gactataatc tggacactta
    24781 tctgcaggcc gggtatgaca gcatagaggc agcagaagtg gccaaaggag atatcaggca
    24841 gtgtctggca aaatggcagg aggatcccgt ggcagccgtt acgtttctca aggagaaact
    24901 gctgatccag tggattgagc ctacttattg cagctttaac cagaccagat accagtaccg
    24961 gccgcaaggc tgggtggaag acctctatac cggacaggcc aatgaaagag cactggcatt
    25021 cctgaaccgt tttcagggaa tggtatatct ggcggttttc gggtattatt tgaggatcct
    25081 gctgggaaaa ctgaaaggtg tacaggtact tccggggatt atttttctgg gcggcttttt
    25141 cattacgatc ctctgggagg ccaagagccg ttatgtgtat ccctatatcg tcatgatcct
    25201 gccctgcgcg gcatgcagta tggaatatta cggccgtctg ctggcgggtg gcatcgggag
    25261 gatcgcaggc ggtattgcga gtagtaggga aaggaaacag aaacaaaaag aaataggaag
    25321 gacagcatga aaaaattaca gattggtaat gtgaccctgg acaacccggt gatcctggca
    25381 cccatggcgg gagtatccga cctgccgttc cgcctgcttt gcagggagat gggagcggcg
    25441 ctggtgtgca tggaaatggt cagtgccaaa gccatatatt ataacaataa aaatacggac
    25501 agtctcatgg agatccatcc cgaggaagtg ccggcttccc tgcagctgtt cggctcagat
    25561 ccccagatcc ttgcggagat ggcaaaacgg atcgaggaac gtcctttttc catactggat
    25621 ctgaatatgg gctgcccggt gccgaaggtc gtgaacaacg gcgaaggctc cgcgctgatg
    25681 aaggatccga agctggtgga gcagattctg accgcactgg taaaagccat cgataagccg
    25741 gtgacagtga agatccgaaa aggcttcgac gatgaccatg tcaatgccgt ggagatcgcg
    25801 aagatcgcgg aagcctgtgg tgtggcagcg gtggcggtgc acggacggac tagagcccag
    25861 tactacagcg gacaggcaga ctgggatatc atcgcacagg tgaaggatgc agtgaagatt
    25921 ccagtgatcg gcaacgggga tgtggacagc ccggaaaaag caaaagccat tctggaacag
    25981 accggctgcg atggtgtcat gatcggccgg gcggcagaag gaaatccctg gattttccgg
    26041 gaggtggtat cctatctgga aaacggcacg atcccggcaa gacccacgaa ccgggagaag
    26101 cgggaaatga tcctgcgcca tgcggcattg cagctggaat acaagggaga atacaccggt
    26161 gtccgggaga tgcgtaagca cctgtcctgg tatacggtgg gaatgcccca ttccgctcat
    26221 ttccgccaga ccatcaatac catggagaaa atggacgaac tgatccgggg agtgcaggaa
    26281 atctttccgg acgaagtgta gtgcggattt tggatacagc aatgcattat gaggcagacc
    26341 ttgcatgata tgatattgtg cgcggtgtgg tgcatataat tgggatgact gcatacaatg
    26401 aagcatggac actattttgt atctctatcc ggcgcgacgg acagcgatgt gcgggacacc
    26461 gccggaggaa aaagcggaac tgcgccgggg aaaactgaag aaatttttgc ccttcctgtg
    26521 gaaaaaggga gaagaccggc gccctgtggt gactgtgagc cggacggaat attttttccc
    26581 ggactatcat ctggtcagag tgtcggtagc cagaacggaa ggcatggata aagaacaggc
    26641 ggagctaaag ctccgggaac aattacagga attgacagag gatccggatc acacctatgt
    26701 ggtgtgccgt gagcctcttt ctttttattt cggaagggaa ttccgggatt atctgcaaca
    26761 ggagtggatc gaacatctgc tgcgctacgg ggagagaccg ggcggctatc cggcgtggcc
    26821 ggatctggtc ctgctgggga gaaatccgta tctgccttat gtcgtgctcc gctatgctgc
    26881 cagattgcag agcatgaaat ggtatcttac ggaaagagaa taccgggagg aggaagagtg
    26941 gctgaccggg gaactggagg atgagtacgg tctgatcccg gagatccggc tgcttgcgga
    27001 aaatgcggac tacggacgag tacggctgca ggggacgaaa ccctgcgtgg tactggattt
    27061 ttgcgacaat gaaaaggtct acggcggcgg actggcagca ggcagtatct ggctggattt
    27121 tccggcatcg gaggacaagg agcaccgggc cctggtacaa tgtccgcaga tccgttattt
    27181 ttccatgaaa aaagagtgga aagaccctcg aaaagcccgg gaataccttg acaccatcag
    27241 caaaaatggg tataataact cggttgatta ggctagtata gttgccggga gtgtcggcag
    27301 tacaaaatat agaaaacagg aaggtgcggt atcatgcagg aaaagaaaaa cattttgacc
    27361 tacgaaggcc tcagaaaata tgaggaagag ttacacgatc tgaaggtggt aaagcgtcag
    27421 gaagtagccc agaagatcaa ggaagcaaga gagcagggag acctgtccga gaatgctgag
    27481 tacgatgcag cgaaggatga gcagagagac atcgaagccc gtatcgaaga actggagaag
    27541 atcctgaaga acgcagaagt cgttgtagag gatgaggtag atctggacaa gatcaacatc
    27601 ggatgtatcg ttaagattct ggatgtggag tatagtgaag aactggatta caagatcgta
    27661 ggttccaccg aggccaacag cttaaagggt aagatttcca acgaaagccc cgtaggtaag
    27721 gcactgatcg gtcataaggt cggcgatacc gtagaagtag agacacccgc aggtgtattc
    27781 gcatataaga ttctggagat ccagagaagt aatgttgact aagtaagata gagtaaggag
    27841 tggaaaaaag tggcagatac acagaacaca aaggtacagg aacaggacat tcaccagctg
    27901 ttgaaggtaa agcgtgagaa gctggcgaac ctgcaggaag caggtaagga tccttttcag
    27961 atcaccaaat atgatgtgac ccatcacagc accgatatca agaacaattt cgaggaactg
    28021 gaaggcaaga ccgtccgcat cgccggacgt gtgatgtcca aacgtgtcat gggtaaggca
    28081 tccttctgta atgtacagga tctgcccggc aatatccagg tatatgtggc tagagacagc
    28141 atcggtgaag agtcctatgc ggatttcaag aagtacgatg tgggcgatat cgtaggtatc
    28201 gagggtgaag tcttcaagac caagaccggc gagacctcca tacatgcggc aaaggtaaca
    28261 ctgctgtcca agagcttaca gatcctgcct gagaagttcc acggcctgac caataccgat
    28321 atccgttatc gtcagcgtta taccgacctg atcatgaatc aggacgttaa ggatactttc
    28381 gtaaagcgtt ccagaattat cagtgccatc cgccgttatc tggacggaca gggcttcctg
    28441 gaggtagaga cccccatgct ggtatccaat gccggcggtg cggcagcaag acctttcgag
    28501 acccactaca atgcactgga tgaggacgtg aagctgcgta tctccctgga gctgtacctg
    28561 aagagactga tcgtcggcgg catggagcgt gtcttcgaga tcggccgtgt attccgtaac
    28621 gaaggtctgg acaccagaca caatccggaa ttcacactga tggagctgta ccaggcatac
    28681 accgactatt acggaatgat ggatctgact gagaacatgt tccgctatgt ggcacaggaa
    28741 gtatgcggta ctaccgtgat cccttatgca gaagagacca tcgacctggg caagcctttc
    28801 gagagactga ccatggtaga tgctgtcaag aaatacgcag gcgttgactt cgaccagatt
    28861 cccgataccg ctgctgcgaa gaagctggca gacgagaagg gtgtacacta cgaagagcgt
    28921 catgccaagg gcgatatcct gaacctgttc ttcgaggagt tcgtagaaga gcatctgatc
    28981 cagcccgtat tcattatgga ccatccggta gagatctctc ctctgaccaa gagaaagccg
    29041 gacaagcctg attatgtaga gcgtttcgag ctgttcatct acggacgtga gatgtgtaac
    29101 gcttattccg aattaaatga tcccatcgac cagagagagc gtttcaaggc gcaggaagct
    29161 gcactggcag ccggcgatga ggaagccaac accaccgatg aagatttcat gaacgcactg
    29221 gagatcggta tgcctcctac cggcggtatc ggttacggta tcgaccgtct ggtaatgctg
    29281 ctgaccaact ctcctgcgat ccgtgatgtt ctgctcttcc cgacgatgaa aactttgagt
    29341 aaagaaaacc agtaaaatca agggtttcag c
//
LOCUS       DAWEJW010000024        29120 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_672_length_29120_cov_60.828694, whole genome shotgun sequence.
ACCESSION   DAWEJW010000024 DAWEJW010000000
VERSION     DAWEJW010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 29120)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29120)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29120
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_672_length_29120_cov_60.828694"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    24146..24245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8280 a         6174 c         7869 g         6697 t
ORIGIN      
        1 ccttaccaga gagagcggct gcatgtatgc gctggaaggc tgcttcaaga caggaatgtg
       61 gaaggtagct tccaagccgg agagccgaga ccggatattc cgggcgtaag ttctcacgag
      121 ttatccccgt taccggatag gactttgtaa gaagtcgcga ggcagcgcaa gctgtgaaca
      181 aaggtggtac cgcgttactg acgccctttg ctgacgagag tcggcagagg gcttctttag
      241 tttcaggaga atttaaggag gaagcaatga gcaaagaatt agccaaaacc tatgacccaa
      301 acggcataga agacagactg tatcagaagt ggcttgacaa aaagtatttc catgcggagg
      361 tagatcacag taagactccc ttcactatcg tgatcccgcc cccaaatatc acaggacagc
      421 tccacatggg acatgcattg gacaacacca tgcaggatat cctgatccgt tataagagaa
      481 tgcagggcta caatgcactg tggcagcccg gcaccgacca tgcaagtatc gctaccgagg
      541 tcaagatcat ccagaagctg aaggaagaag gcatcgacaa gcacgatctg ggacgtgaaa
      601 agttcttaga gagagcctgg gagtggaaga aggaatacgg cggacgtatt attagtcagc
      661 tgaaaaagct gggctcttcc tgcgactggg acagagagcg tttcaccatg gatgagggat
      721 gcaacaaggc tgtgaccgaa gtgttcgtga agatgcacga aaaaggctat atctacaagg
      781 gtgctcgtat cgttaactgg tgtcccgtat gtaatacctc catctccgat gcagaggtag
      841 aatatcagga gcaggcagga catttctggc acatcaagta tccgctgatg aacgaggacg
      901 gaacgcccag caccacagag tttctgacct ttgccactac ccgtcccgag accatgttag
      961 gtgataccgc agtagccatc catcccgatg atgagcgtta cacccatctg catggcagaa
     1021 aggtactgct tcctatcgta aaccgtgtga tccctatcgt tgaggatgct tatgtagaca
     1081 gagagttcgg taccggtgtc gttaagatca cccccgctca tgaccccaat gactttgagg
     1141 tgggcaagcg tcatgatctg cctgtgatca atatcctgaa cgatgatgcc accatcaata
     1201 aaaacggcgg caggttcgag ggcatggacc gttacgaagc aagaaaagcc atcgtggaag
     1261 aactggatca gatggggctg ttagtaaaga tcgaagacca tgtacacaac gtaggtaccc
     1321 atgaccgttg taagaccacc atcgagccta tggtaaaaga ccagtggttc gttaagatgg
     1381 atgaattgat caaacctgca agagaagcag tgaaaaaggg cgagatcaag ctgatccccg
     1441 agcgtatgga gaagaactat ttcaactgga cggataacat ccgcgactgg tgtatcagcc
     1501 gtcagctgtg gtggggacac aggattcccg catattactg cgatgactgc ggtgaagtag
     1561 tcgtagccaa ggagatgccg aaggtatgtc ccaagtgcgg ctgcacccac tttacacagg
     1621 atcccgatac gctggatacc tggttctcct ccgcactgtg gccttttgag accctgggct
     1681 ggccggagca gaccgaggat ctgaagtatt tctatcctac cgatgtcctg gtaaccggtt
     1741 atgatatcat cttcttctgg gtgatccgta tgatcttctc cggatacgag cagatgggag
     1801 agaagccttt caagaccgta ttgttccacg gactggtacg tgacagtcag ggacggaaaa
     1861 tgtccaagtc tctgggcaac ggaatcgacc ccctagagat catcagccag tacggtgccg
     1921 atgcactgcg tctgaccctg atcaccggca acgcaccggg caacgatatg cgtttctact
     1981 atgagagagt agagaacagc cgtaacttcg ccaacaaggt atggaatgct tcccgtttca
     2041 tcatgatgaa catggagcag gcactggaga agaccggaaa agatatcacc acccctgcgg
     2101 cagcagattt acagcccgta gataagtgga tcctgtccaa gttaaatact ctggtaaaag
     2161 atgtgaccga caacatggat cacttcgagc tgggtatcgc cgtacagaag gtatatgact
     2221 ttatctggga tgagttctgt gactggtaca tcgagatggt aaaaccccgt ctgtacagca
     2281 ccgacgatgc agtcagccag aatgcggcac tgtggacgct gaagactgtc ctgatcgacg
     2341 ccctgaagct gttacatcct tacatgccgt tcatcactga ggagatcttc tgcaccattc
     2401 agaatgaaga agagtccatc atgatcagca agtggccgga gtacagcgag gatcgtgcat
     2461 tccccgcaga ggagaaggcc atcgaaacca tcaaggaagc cgttcgcggt atccgcaaca
     2521 tccgtactga gatgaacgta gcacccagcc gtaaggcaag tgtgtatgta gtcagcgaaa
     2581 atgacgagat ccgcaggatt tttgaggagg gtaagctgtt cttcgcaagc cttgcttatg
     2641 cgaatgagat catgatccag gctgacaaga ccggaatcgc agaggatgcg gtatccgtag
     2701 tgatccccgg tgcaaccctg tacattccgt ttgcagagct ggtagacatc gcacaggaga
     2761 tcgagcgact gaagaaggag cagaagagac tggaaggcga gctgtcacgt tccaggggaa
     2821 tgctgtccaa tgagagattc ctgtccaagg ctcccgaagc gaagatcgca gaggagaagg
     2881 aaaagctggc aaaatacgag cagatgatgg aacaggtgac aaagcgctta gaacagctgg
     2941 catgatctgc aacgaaacgt acagaataaa aagaacgtaa agcataagga atacgatgag
     3001 attcggtaat gacatgataa cgtttgaaga agcggtggca tatctgaagg atatgccccg
     3061 ctttactgtt aaaaaaaatc ctgcggacag cagggacctt aaatggtttt taaaaaaaat
     3121 cggaaatccg gagaaggatc tgcggatcat tcatgtggcg ggcaccaatg gaaaaggctc
     3181 cgtctgtgcc tacatgagtt cgatcctgcg ggaagcaggc tatcgcacgg ctgtattcac
     3241 ttcacctcat ctggtggata tgcgggagcg ttttgccgtg aacggtgaaa tgatctcaga
     3301 ggaagctttt ttacaggggt ttcttgcggt atgggacgct ttgcaggaga ccaattctgc
     3361 taatactggg gacgggcagc gaggggaaga agcgccacag gaattcgttc tcaactttta
     3421 tgaatacctt ttctgcatgg cattactctg ttttgcagaa gaaaagcccg actactgcat
     3481 cattgagacc ggcctggggg gcagactgga tgccacgaat tatgtggaca ataagctcct
     3541 gaccgtcatt acgcggatca gtctggatca tgtacagtat ctgggagaca ccacagcgaa
     3601 gatcgctgcg gaaaaggcag ggatcctgcg ccccggtgtt ccggtggtgt atctggacgg
     3661 ggatgcggat gccagtgcgg taatttgccg caaagcgtca gaacttggtg cgatacagat
     3721 accggtgtcg aaaaaggact atactttcct tggatttcgg aagaaataca ttgatttttc
     3781 cttgcgctct gagtattata attatattag tcttactttg cataccattg cacgctacca
     3841 gatggaaaat gcggcattgg cagtgcgggc cgtggaagtg ctgtttcgca gtacggacat
     3901 gcctgacggc agaatgtgtg ccgcagccgg gtgtcccact gtggaagaga tccgacaggg
     3961 aatccttggc tgcttctggc agggacgcat ggaagaagta cttccggagg tgtacgtgga
     4021 tggtgcgcat aatgatgacg gcatccgggc ttttctggat accgtggaac aggacggctg
     4081 cacggagggc aggagactgt tatttggtgt ggcggcggat aaggattgca ggcatatgat
     4141 acagagggtg atcacgtcgg gattattcga ccggatcgct tttacgcata tgcggacggc
     4201 gaggtcgctt tcgctggaag aactgaaagg tttactggca gcttatccgg aagaccggtt
     4261 tactatgtat acggaagcgg atacggcact gcggacggaa gtctcgggca gacagccagg
     4321 agaacggctc tatatcgcgg gaagcctgta tcttgtcggg gaaataaagg agtctttgga
     4381 ccatgatcaa ttttgaagaa gaactgaaga aatttcatcc cagtctggaa gtggaagacg
     4441 ctgaggaagc tatctacaat caggatctga ccgatgcggc ggatctgttg gtgaaaatga
     4501 taaaagaatc cgaagaaaaa gaagagaaat aaggagggag agcatgtttt gttataactg
     4561 cggctgccat ctgtcagagc acgatttctg tacgtcctgc ggagcagatg tatccctcta
     4621 caagaagatc atgtgtatgt ccaaccgttt ctacaatgac ggtctggaga aagcaggagt
     4681 acgggatctc acaggtgcga tcaacagctt acggcagagc ctgaaattta ataagaacaa
     4741 tatcgaagcc agaaatctgc tgggactggt gtattttgag accggagagg tcgttgcggc
     4801 actgagcgaa tgggtcatca gcaaaaatat gcggcctgag aaaaatatcg cggacgatta
     4861 catcaatatg ctgcagtcca atgcggcccg cctggatgcc attaaccaga ccatcaagaa
     4921 atacaatcag gcactggtct attgtaatca ggacagcaag gatctggcta tcatccagct
     4981 gaaaaaggta ctgtccctga acccgaagtt catccgggca caccagctgt tagccctgct
     5041 ttatatggac agtgaacagt gggacagagc ggagagagaa ctgcgtaagt gcatggacat
     5101 cgaccgcaac aatacgctga ccctgcggta tctgaaggaa gttgagatgc agctgactcc
     5161 ggacgagaat agcaagcaga ccggccggcg gaagaaggat gatgcggttc gctaccagag
     5221 tgacaatgag atcatcattc agcctttgaa tgtgaaggag cagaagagtg caggtctctc
     5281 ttccctgatc aatattgcca tcggtctgat cattggtctg gccgtgatgt attttctggt
     5341 ggttccggca gcggtatcca atgccaacaa tgaggcccag aagaccatca ccgacatcgg
     5401 caatcagtta gacaccaaga cctcccggat ccaggaactg gagacccaga tgcaggcact
     5461 gcagacagag aatgatacgc tgaatcagga actgcagggg tatgtgggaa ccgacggtac
     5521 gctacagacc atcgacagcc tgttagcggc agccaccaca tatctggaga cgcaggatat
     5581 tacccagacg gcggctaacc tggaaagcat ttcggcatcc gtggatgtgg acagtacttc
     5641 cgaagcattc cagaagctgt acaagacgtt gcttggcctg atcgggccgc agctgtccgc
     5701 acagtattat gagacaggct acaatgctta ccgcagcgag gattacgcga cggccatcga
     5761 gaacctgtcc aaggcagtca tttacgatga gaccaacaag gatgcctggc tgagcctggg
     5821 caactcttat cgtaagagtg acgatgccca gaatgccatc atcacctatg acaagatcat
     5881 agagctgttc ccggatacgg agacggccag aagtgcccag agatatcgtt cccagctggc
     5941 agagaatacg gcacagtagg gaagaacaac ataccatcag acaatgacac acaacacacg
     6001 aaagagaact tgcgtaaagt aaagcgcggg ttctcttttc ttttgagtaa aaatgctgga
     6061 aaacagcgga atgtgaggaa atatggaaga ctttcagatc attgataact gcctgatggt
     6121 gcgcatgccg gaggaagtgg accaccacag agcctcctat atctgcgaag gggcggacag
     6181 gctcttggtc agggaaaacg tggaaaatgt agtgtttgat tttgaggata cccggttcat
     6241 ggattcttcc gggatcggga tcatcatggg gcggtaccgc aagatttcct gcttcggcgg
     6301 acatgtctat gcgatccatg cagaccggca gatccagcgg atcttccgta tgtccgggct
     6361 tcacaagatc gtcaagatcc tgccgggtta aacgttggaa ggaagattct gtcgaaaatg
     6421 aaagaaatta ttcaaaaaat tatgaaaaac agtgcaataa taggcgcaga aatgaggaga
     6481 acatggtagc agagagggaa aacattcagg tgacggaaga aaaagaactt ttgcagcaga
     6541 gaacgcaggg cagccggaat gagatggaaa tcgtatttga tgcggtctcc tgtaatgaaa
     6601 gctttgccag agtggcggtg gcggctttca ttacccatct gaatcccaca ctggaagaac
     6661 tggcggatat caagaccgca gtatcggagg cagtaaccaa tgccattatc catggatacg
     6721 agaatctgtc cggttatacg aagcatgggg agatgcgccc ggcgtacagc attatccatc
     6781 cgggaaaggt aagactgcac tgcattcttg agaacgatac gctttccatc gaggtatcgg
     6841 atcagggaaa aggcatcgag gatgtggcca gagccatgga accgttattt accacgaagc
     6901 cggaactgga acggtccggc atgggatttg cctttatgga agccttcatg gatgagctgg
     6961 aagtggagag catcccgggc aagggcacaa gagtacatat gtggaagaaa atgcgctgcg
     7021 gagactggat cggaaaagag gactgaggca tggaagaagt atcagtactc atcggaaagt
     7081 cacaaagcgg agataaagaa gcgagagaag tactgatcga gaaaaatctg ggactggtgc
     7141 atcacattgt gaaaagattt ctgggcagag gttatgaccc ggaggatctg ttccagatcg
     7201 gtgtcatcgg tctgatgaaa gccattgata aatttgacct gaagctggaa gtgcggtttt
     7261 ccacgtatgc cgttccgatg atcaccggag agatcaaacg gtttctgcgg gatgacgggt
     7321 tactgaaagt gtccagaacc atcaaggagg atggtctgaa ggtcaggctg gcaagacagc
     7381 ggctgcagag cagactgcgc agggagccta ccctgcagga gctgtccgag gaggcacagc
     7441 tggacagaga ggatgtgatc ctggccatgg aagccggggc ggaggtggaa tccctctata
     7501 gcagcagcgg acaagaggat ggcagcgaac tgtgcctggc ggaccgggta gtggccgcgg
     7561 cccacggctg tgtgggagcc agcatgggaa gcagcagtgc ggtggaaaat gatgtggaga
     7621 aggacagact gctggaccag atgctgctgg cacagctgct tggggagctt ccggagagag
     7681 accgggagct gatccgtatg cgctattttc agaataagac ccagacggag atcgccggga
     7741 tcctgggaat cagtcaggtg caggtcagcc gcctggagaa aaagatcctg cgggaaatgc
     7801 gggagatggc cggataaggg ccaattcatt aataattcat aaaacaaatt ttttcctctt
     7861 cctttccgtc ggaaattcga ttatattata tgaaggaact attcagcccg cattcgcaaa
     7921 accgctcttt cagagcttgc tttttgctca tgtggttatt tcgccgtata aattatgtca
     7981 aatgttctta cgaatgcaag cattcgacgt acatattgcc ataatttgca cggctctgaa
     8041 tagttatata taatcttata gaccgcaatg ctgcatagtt atagagagac agcatgaaaa
     8101 aggagagcag gggtacttat gaaaaaacaa aatgaaaacg gacggatccc cgaggatcgt
     8161 ctgagcagag caaataagcg taagaaaaac aacaccatag catccattct ggtcatgacc
     8221 ggatgcctgt tggtgttagt actggtaacg tatgttgcgg gaatcctgta ttcctggatc
     8281 gacagcaagt tccagaataa tgccaacgat ctggcggcag cagcggaagc cggaagccag
     8341 acggaagaga gcacacagga agtggtgaag acctatacgc aggaggaagt ggatgccctg
     8401 atcgctgaag caaagcagca ggctgaggac gaggaagaga acaagatcct ctccgggatc
     8461 cgtgatggtc tgacctccgg cactaccatg gtagagaccc tgcgtcctta ctatcccaat
     8521 gaactggtcg ttgtgagcaa cggaaccttt aatttcgtac ccattcgtga tgatttgcag
     8581 aaaaatgatt atgtattgga gaacctgaac attctcgagg acggagaagt gcagtatatg
     8641 caggacggtc aggtagtatc ccacaaggga atcgacgtct ccaagcatca gggcaatatc
     8701 gactggacca aggtagccgc agacggtgtg gaattcgcat tcatccgtgt aggcctcaga
     8761 ggctacggca ctgagggtaa gctggtggag gacgaatact ttgagcagaa tgtcaaggga
     8821 gcattacagg caggaatcaa agtaggtgtc tacttctact ctcaggccat cacagacgag
     8881 gaactgttgg aagaagccaa tctggtactg gaaaaggtaa aaccttataa catcgaactg
     8941 cccatcgtct tcgacgtgga aaaagtcagc ggcggcaagg gccgtgccaa cgaactgagc
     9001 gtggaagaga gaacccgcct gaccgccctg ttctgccaga ccatccagga tgccggctac
     9061 aagcccatga tctatcacaa catggagatg ggaacactga tgctggatct gggacagctg
     9121 gaacagtacg ataagtggtt cgcttattat aatgatgatc tgtattatcc gtatgcctat
     9181 aaggtatggc agtacagcga aaaaggcgct gtggacggta ttaatgagga agtggatctg
     9241 aatatctggt tcggggactt ctaaagggtg attacgatga aagagaagat ctagatgaaa
     9301 gactgtagaa ggacattact ggacgaattt gtgaagctgt cgaaggattg ggacaacaat
     9361 aatatgttat ataattctct tatgttttct aaattatatc ctggagatgt tgaaaatagc
     9421 gtagcagatg cttcactgat gccgaagcag agtgatgaaa agatgagaaa tgatatcatg
     9481 acaagctggt ggactccgac taaaatcttt ttattgggaa ataagaagga gttaatgcaa
     9541 aaatcaagga aagaattgga ggaagtctta ttggaacgga ttcctatggg gaaggatgaa
     9601 gagcaactcc gagaaagctt atcaaaaatt cggcatgaaa aaacagggga aaaaatagaa
     9661 aaagacgtta tagatgcatt tatggggttc cttggatcag tttatgccgt gggcaatatg
     9721 acaagtgcag cggtgacatc caggggtggt gccttggata attgggatgc aaaactgaaa
     9781 aacatacatg agaagtatat aaaagaagaa aaagcgtggg ttgattatgt aaagaccaat
     9841 aaatttgagt gttattttgt ggataaggat ccgcgtaagg aaataatccc attttggagt
     9901 tacggttaca gcaatttagc atctgcaacg gatgctgatt ggatggaata tttttccacg
     9961 gttacaaaaa ggattgaaga aagagacaaa ttgaaagcct gaatagatat tcattcactt
    10021 gcgaatatag ctcctataat acaaataatg atacttttta aaagtaagac tttaaatgag
    10081 acggttgcgg ccgtctcgtt ttatgtagcc agaaattttt tataacctta tccatatccc
    10141 catatccaaa aatcatttta ttttctatat atactaccac ttatttacaa aaatattgcc
    10201 atagatgttg tggagaaaga gaggaagaga cggggaggaa agcattatca ggcatctatg
    10261 atgcatatga tattcggagg aaatccggga agtagcaaaa ggaggcgtat tattcattga
    10321 tgaagcatat tctctgcaag gcgagatcgc tattacgaca ttgcttcggg aaatggaaaa
    10381 ccacagagag gatgtcattg tgatatttgc cgggtacccg gaacctatgc aggggttcct
    10441 tgacagaaat ctcggtatgc ggtccagaat tgtatttcag gtgaaatttg aggactatta
    10501 cagccagaac ttcccggcag ggaggaatgc ataggaataa gcagtgggaa cagcatgcat
    10561 aaagccggtg gagaatgtct gacatgggat tcttgcgaga ttcctgcgga ggaagaggag
    10621 cgggtggttc ccagagaaag gaccgctttg aatatcagtt tttccgaatc ccctgtatct
    10681 ggaggcagat tttccggaga tggaagaggt gcaggatgag aagcagagag cacaccgggt
    10741 tcgcgggtga ggcagaaact atatgggaca aagtatatta aggagtctga atgagcagaa
    10801 gctataagca tacgccggta tatgaggcga aggggaacag aagcaaggag cgatcccgct
    10861 tccagaaagc tcgacagata tcagaagtgc taccgaagga aatgagaaaa ggaggaagga
    10921 tatgcattat gttatcggcg acatacataa cgacctgaag aaactgaaac agatcctaaa
    10981 gcagatcaat atcaccaggg atgatgaact gatcgtcctt ggtgatgtgt ttgaccgggg
    11041 tggagaaact gcggacccct tcggagtata ttgtgccctt gccggcttgc agggaaagtg
    11101 ctcctggata cgaggcaacc acgatgactg gctggcaaat tatacttatg agtactttaa
    11161 tactccggag aaaaaacggc acaaattagc tccctatggt tacaacagtt ttgaactgct
    11221 gcgacagcgt attaccgaag tagacatgcg gaacattgct gatctgatca agggactgcc
    11281 gctacagaag gaactggagc tggagggaaa gaaatatctg tttgcccatg ccatgacttc
    11341 ccatccggat gtccgggaag aggaccgata ttacatgcgg gggacgatgg atattgactg
    11401 ctttttcttt gaaggtatcg atggatatat ctccatgtgc ggtcatacgc ccacggacag
    11461 cattgtgtgg aagaaggaca gaagatatct ggacgagccg ggcaattcca tctggtgcaa
    11521 cgacagacat aacgtatatc tcttagattg cggatgtggc tttgccggtg gaagacttgc
    11581 ctgtctctgt ctggagacaa aagagcgatt ttacgcctga aaacgggacg aggatattta
    11641 tgtacaggaa ttatttctac agggaggatc ttaaggtaga actgacacag ttggaagaag
    11701 aggatgaaaa agagcggttc cttgagggaa gtaaacataa gaggctgatt cccggttatg
    11761 ttgcgttgga agtcaaagaa aatacctgta agatatctcc ggtgctgtat ccggagtttc
    11821 gtgggaaggg atactggaaa gatgccagag aacttttgct ggaaagaatc tttaaagaaa
    11881 gatttttcac agaagaaaac ggaagacgtg ttccttaccg gatcgaccgg gtggcgatcg
    11941 tattgtatca gaaaccccga gatattgccc ggagtacgga aggaaagctt ttgtttcggg
    12001 atttgaaacc ttatttacag atattcggat atttttctgt agtggacaga gacaactggt
    12061 ccggtgaaat gtataagtac agagatgaca taccggagaa atacgatgac aggcccgtat
    12121 atggaatcgg tatggaggat aatcccgatg cggatacatt gccgccaaag cggctggtga
    12181 tcgtgctttc agagaatgaa aaggaagaat catgaaaaag tttatggaac tgttggaatg
    12241 cgatttccgt agcggagaaa acaaatattg tcttttaaaa tctccggaag gctttacttc
    12301 ttttcagata cacgaggatt acggaaaaat cttctggatc agagatgacc tgtatcggga
    12361 aaattaccat aatgtgatcg aaccgctgat ccattatccg ggagatcgaa gatttctgag
    12421 ttctccgatc tgcggatgcg acgagaatcg gatttagaat ccttccttct tacgaactaa
    12481 tcgtaactct cttctcttaa tggcattatc ccattccatg cgttccccgt cgttctctaa
    12541 aatgaggccg ggaaccggag tgggctttcc atcgtcgccc atggctacca tgacgaagta
    12601 agcccggttg atgggatgtc tggtgccgtc ctgctcttcg cggtaggtgt ccacgcggac
    12661 ttccatggaa gtgtgtccca catgggtgac tttaccgatc agcacaataa tgtcattcag
    12721 ataggcaccg gatttaaaat acaggttgtc gatggctacg gtggtggcat ccctgccgca
    12781 gtgacgtctg gcagtggctc cggctacttc atcgatccac tccaacagtc ttccgccgaa
    12841 caggcgcccg aaaccgttga tatccggatg aatgaccaca tgcagctgat gtgtctcgga
    12901 atcggagact tttttcgcgg gaagtgcact cgtctttttt tctgtatttt cagtcgtatc
    12961 tgctgtattc atagttgtaa aacctcacat atattttgaa atctaaaatt cttcactcaa
    13021 aacggcgcaa acagtaagtg gggcagcgtc tgcacatata taaagatagt atagcacatt
    13081 tgggaaaaaa gtgccatacg tatatgattt tcggttttgg gatattctat tgtgtgcaga
    13141 aaaagatcat ttgcagatcc tttgataact gccgggagca gaactttgcg aaaggggaac
    13201 ggagatgctg atcagacaat tattgctcat agtaaccggg gccagttacg gcctgttgtc
    13261 ggcagccgga gtgtttacag tgctggtggc ggtggggctg atcccccggt tcgcaggcaa
    13321 gacccatacc gcacgttatg tacttctcta tgaggaaatg gtcattttcg ggacgctggc
    13381 gggatgtttt gttacgattt tcccagagta cagtcagtgg ggaaattatc tgcgggaaca
    13441 cttcccggaa cagatgaggc tgtggacggc agcgggcatt ggagcacagg cggttttcgg
    13501 gctgtttgcc ggaatgttca tcggttgtct ggcgctggcc attgctgaga tgctggacag
    13561 tattcctatt tttgccagaa gaatctcttt ccgccacggg ctgggcgggg ccattctggg
    13621 gatggcagcg ggaaagatct gcgggtcact gttttatttt gccacagaac tgcatcggac
    13681 cgtgcaataa gcaggggaat tcttccggag gagcaagcac acaaatgata tggacaatct
    13741 gggaaaatat gtctatgcag agcagaaacg gcaggaaggg acagggtata tgaagcagca
    13801 gacggtttat ctgaaatgtg acagaagtgc ggaggtgcag gcacaggatg ttttcctgaa
    13861 agatgtggca gagattcgct gtcaggatcc ggtattgcag gcaaaactaa aagccatcaa
    13921 aatttatcat tttcccaagg acggcgaaaa acgatgtgtg atcagttgtc tgaaactggt
    13981 aagtcttatg gaagaaatct gtccggagat cgatgtgcag gtggttggag agacagatgt
    14041 gctggtggaa tggatctgtg tgaacagaca caaaggctgg cagcagtgga tcaaggctgc
    14101 ttttgtgtgc ctgatcagct ttttcggtac agcctatacg atcatggctt accataatga
    14161 tgtggggatc aatgaggttt tcacggaagt gtaccggatg gccatgggtg ctgaacccgg
    14221 aggcttgaat acgttggaag tattctattc tcttgggctg gcagctggca ttatcgtttt
    14281 cttcaatcat gtcggtggca ggcgcctgac caaggatcct acccccgtcg aagtctccat
    14341 caaaaactac gagaccgacg tggataagac cctgatcgaa caagcaggaa gagaaggaaa
    14401 agagctggaa agctgacctt cccttatttt agcggaatca tttcggaata agaggtattc
    14461 ccctgggaat ccttcatgaa gaagatgcat acgtaatttt ccgggtgatc cagcggctgt
    14521 accacggtat gataatcgcc ggtcagatcc acatcgaagc ccatcatcca gccggatttt
    14581 tcccagtcga agaagttcaa cagattaccg ttatcatccc gggtcacgta gctgcatctt
    14641 gccattaagg acatagtctc atagtcatcc agatggatca actgcttgct ggcactctgc
    14701 aattccgcat cgtcatgatc cagaggaatc gcactacgga tgataccatc gggatattcg
    14761 tcgctgacga cgatctgtac tttcgccgtc tgggattccc agttgtcagt ataattcatc
    14821 agcaccgcat gtgccagata acgattctct gtgtcgctga tatcggtctc tgtcaggatc
    14881 atgggaatat cggagagacc gtcctgatcc tgcatgtaga cagcgttatt tttgtaagca
    14941 gcatgtaaga aaccattttc atccagagtc acacgggtac ttccctgaat gaagagatac
    15001 atatcatcgg cacgttcgtc ttcctccgcg gtaatatagc cttcgtccct ggctttgcag
    15061 aggatgtagt agccgccgga ggcaaagttc gcctgctgtt cgggggtcag ctgcaaggta
    15121 atgtcggatt catcgtaagc accgtcgttc tgggtggtga cggaagtctc cgcattggag
    15181 atctctcgat ctgccagcag ggtgtcaccg ttttcaatgg cgtaaaagtc ttccagaaaa
    15241 cgggtgtaat tgggagcaaa gcccagataa tataacattt cgatgcaggc atcccgataa
    15301 tccgcatccg tctgataagg atagcagata gagattcctc cggcattgtc catgttggtg
    15361 ccagaataga cgatacagtt ttccacggcc tctgcggcag cctgtgcggc ttcggaatca
    15421 tttcccacca gctctaacag atgcaggaca tccaccatgc cataatccat atcggaggag
    15481 taagtaccga agtctcttac ccgggcacgg ttacgcacca gcctggggta attctgcaca
    15541 tccagagaag tgtccagttc cgcaaaataa tcgttcagag cttcttcggc ttcggcatag
    15601 gcattcaggt cgatgcagga cagggtcagg tcggagtaca gattcggata gtagtcgaag
    15661 acatattcac cgtaatccat gtaggaatcc acgatatatt cccccatgac atcgcccggg
    15721 atcacctcat cggacagctc tgacaggaaa tcgtaattcc agccccagcc gggttcggtc
    15781 tcctgggagg caatcatata attggcatag ggagccagca cggaaaccac ttccagggaa
    15841 ctcatcaggc aggcatcaaa gccgatccat tccagttttg tcatgtgggc gcccacggaa
    15901 tctcccagcg cctcggacag ttcatccagc gtcagaaggt cgcggaagtt ctcatccacg
    15961 ccgtagccca gcaccggtcc gccaccgtga tcccagagga tcagactgta ggaatctgcc
    16021 gggaagaaat caaaaccgta atttaaaaag ctactcaaag tcaggggatc ccccatgttt
    16081 tccgccggat aggtatccac ggggacgaaa ccggtatcgg tcaacagcag gacggtgcac
    16141 tcgtcaccgg acaggccacg gtcctgccat tcggatgcac caccggcata gaccagaata
    16201 ttattgtgcg ctgtgtccac tccggccgct tccatctcgt ccagatccag actggcatta
    16261 ccgtaatcgg attccagatc ggagcctacc agatagacca tgatggtctg gacttcaccg
    16321 atggtatcca gcgagtcgta atccatctgg ggcataggag tggaagacgg ttgggactcg
    16381 gtatggtaag taccatgacc gtgagacgag gatgcctgcg aattgttttg tccggcatac
    16441 tccatgatct gtgcaaatac gcccacaggc gtggtgcgga tgcggcaccc ggtcagaacc
    16501 acggggacca gcagcagacc caaaagggcc cgtaactttt tctttgttct ttttttcata
    16561 ataatttccc tcatgaaata aaatttatac tcattgccca ttatcgcatg aaaaaaagag
    16621 agcgtcaaac agtgtaactg cttacaatct gaaaaaatac caaagaaaaa acaaaattct
    16681 ggttctttct tttttcggaa aaagatgggg agccggaatt cggtggacag tcagaaccac
    16741 aggaaagaag aaaagacttt tttacaggaa aacggtgtgg acctcatgat ccgggtacat
    16801 gacaccggaa tcggtatggc catcacggat cagctggtac agctcatggg cggagaaatc
    16861 gtcgtagaga gtctgaaaga taagggctcg gatttcaccg tgtttttgca gctgcctctg
    16921 gcagaggaag gcagtatcga agagaaaacg acaacagaga atcgggaaca gaatgcggat
    16981 aacgcgttcc gcggccgcag gatcctgctg ggcggaggtc tatttcgacg gtattggatg
    17041 gcttccggtg gatgtgacac cggggtatta ttatgacgcg gtgaaattgc agcagatggt
    17101 ggcgacaccg gatatggtgc acaagacact ggcagaagat cataatctta tggatgcgga
    17161 acagatcacg gataccggaa atgatatgga taccgccgtg gcagaggtcg cggagctgtt
    17221 gagaagtctg tttttatgga agaaaactgt gttaaaagaa aatttgtaaa tagaagcata
    17281 cataaaaaga gtgaaaaagg acattctgtt atcagagaga gcagtcacag agtctgcctg
    17341 ccgtgcaagc ggccgggcgg cggaaaggca tggtacatct atgaaacagg aagacacaaa
    17401 acagggcaat cagcagaagc aggaatacga acagtatgtc aaatcggtga cccccaccca
    17461 caatctgtgg gcacagatgg gaaaagcgtt tctgaccggt gggatcatct gctgtatcgg
    17521 gcagggaatc ctgaactata ccggcaatgt gctgggaatg gatccccaga cggcaggcag
    17581 ctggtgttcc ctgatcctgg tgtttgcatc ggtggtactc acggcactgc atatttatcc
    17641 caagatcgtc aaatggggag gtgcaggagc cttagtaccc atcaccggct ttgccaattc
    17701 cgtgacagcc ccggcggtgg aataccagaa agaaggccag gtattcggta tcggctgcaa
    17761 gatattcacc attgcaggac ctgtgatcct gtacggaatt ttcgccagct gggcattggg
    17821 actgatctgc tgggtgctgc agatgttcgg gatccaattg taagttgaac tgaaatagaa
    17881 aacagtgtaa aatgactttg tctggatgcg gtttcgggca aagttttttt atgaaatgtt
    17941 aaaggagaga ttaccatgca gataggaaaa gagatggcga tcccgtccat cctgaagatc
    18001 ggaaacgggg cattaggtaa gatcggtggt tatttaaaaa cggaaggttt ggatcaggta
    18061 gtgttatttt tcggtaacgg actgatcgat atgttcggag agctggtcat gaattcactg
    18121 aaggaagcgg acgtgaatgt tctggaatac aatgaattgg atacggtgga tattgacgat
    18181 atcattacgc tggctttcgc tattcccaac aaggcaaagg cggtgatctc tatcggtgga
    18241 ggcaaggtca tcgatgccgg aaaatatgcg gcatttctgc ggaaccttcc ttttatcagt
    18301 gtgccgactt ccagttcaag cgacggcttt tccagtgcca gcgcatccct tctggtgcag
    18361 ggaaagcgaa cttctgtccc tgccagactg gcttatggca ttatcgtgga tacccaggtg
    18421 atccgtaccg ctccggagaa gttcatttac tccggcatcg gagatatgat ttccaagatc
    18481 acggcacttt atgactggat tttcgaagaa aaggccggct gcggtgaggt gaatgatttt
    18541 gctgtgatga tcgccaagaa agcggtgaac agcttcgttc gtacccctta tgagagcatc
    18601 aaggacgagc tgttcctaaa agaactgctg gattctttgg ccatgagcgg tattgccaac
    18661 gagattgccg gcagcagtgc ccctaccagc ggaagtgagc acctgatttc ccatgcattg
    18721 gataagatcc tggaagtgcc gcagcttcat ggcatccagg taggtattgc cacctatatc
    18781 atggcaaagg tacaggatca ccgttacatc cgtgtcagca cggtacttaa agataccgga
    18841 ttctgggatt acgtggcaac cttacatatg aagaaggccg atttcctcaa agccatcgac
    18901 atggctccca ccatcaaacc ccaccgccat acctatctcc acgaggaaaa ataccgcgaa
    18961 gcagcaaaga aactggtgct ggaagatgaa gttctgaacc gggtactgga ggattgacgg
    19021 agtttataaa gagtttatac tttttttctt gcaaacataa aaggttgcgt tataatgtta
    19081 gatggctaac aaaaatggtt agctatctaa catttttatt tacgcgagca acgagccaaa
    19141 gtccgtgctt gcacgagacc ttggcgactg ccgcgataac ttgagaaact cgcgaagcgt
    19201 gtttctcata gtttgcgcga gtagagagga aaatcccatg ctggcatgag gatttgacga
    19261 acaccgcatt agaggcatag gagataagga ggcaggatga acgaggagac aagtgtactg
    19321 acgaaccgtc agcttggatt tgagatcaag gccgccaaca acatgatccg ccggaagatc
    19381 gatgcgatct ttgctcagat ggaaggaaat gaaatcaacg gaatgcaggg gccgctgttg
    19441 ggatatctgt atcacaagag ccggaacggc gatgtatatc agaaggatgt ggagaaggaa
    19501 ttccgaatcc gcagatccac ggcaacggta atgctccaaa atctggaaca gaagggctat
    19561 atcgtccggg tggcatccac ggaggatgcc cgactgaaaa ggatcctgtt gacggagaaa
    19621 gcgatcgagc atcacaatct gatcgaagag cagatccggg atttcaatcg agagctggag
    19681 aacggtatta ccgaagaaga gaagcagaca ttcttaacta ttttagataa gatgatgcag
    19741 aatctggggc aggacagcag gcggaccgga cgttgtaacg ggccatgccg gaacgagtga
    19801 tgctggcagt catgcataac agagaggtta caaaacattt attataggat agaaaaatac
    19861 aagaaaggaa accagctatg atcaaagcat tagcaaaaca gatcaaggaa tataagagtg
    19921 catctcttgt aactcctatt ttcatgatcc tggaagtagc catggaaatg gtgattccgc
    19981 tcctgatggc atccatcatt gatgacggtg tgcaggcggg agatatgaaa catatttttg
    20041 ccatcggttg ttacatgatc ctggcagcta ttgtcggatt gttcgccggt gtcatgggtg
    20101 gtaaatacgg tgcgaaagca tccaccggtt ttgccagaaa tttaagagag gccatgtatg
    20161 agaacattca gaccttctct ttctccaaca ttgataagtt ctccacggca ggactggtca
    20221 cccgtatgac cacggatgtt accaatatcc agaacgctta ccagatgctc ttacgtatgt
    20281 gcttccgcgc accggtcagc ctgatctgtg ctatgctgat ggcgttcctg attaatgcaa
    20341 gagtggcaag tatttatctg gtggcggtag tgttccttgg aatcgttatc atttttatta
    20401 tgagagcagt gtccaagtat ttcagtgaag tattcaagaa atacgatgat ttaaatgcca
    20461 gcgtacagga gaacgtagcc gcacagcgtg tggtaaaggc ttatgtgcgt gaggactatg
    20521 agatcgacaa gttccataag gccagctaca acatctacaa gatgttcaaa aaagcagaat
    20581 gtaccatgac ttatgtatgg ccggtaatgc agtttaccgt atacggctgt atccttggaa
    20641 tcagctggct tggcgctcac atgatcgttg ccagccagtt gactaccggt gaactgatga
    20701 gcctgctgac ctattgtatg acgattctga tgaacctgat gatgttagcc atgatcttcg
    20761 ttatgatgac catgagcgct gccagcggaa agcgtatcgc ggaagtatta aatgaaaagg
    20821 cggacatcac caaccccgaa caacctgact acgatgtgac agacggtagt atccgttttg
    20881 atcatgtgac cttccgctat aacaaacaga gtgagaagcc ggtactggat gatttgaact
    20941 ttgagatcaa agccggagag accatcggta ttctgggtgg taccggtagt tccaagacca
    21001 gtctggtgaa cctcatcagc cgtctgtatg atgtaaatga gggtacggtc tatgtcggcg
    21061 gtaaggatgt cagaagctat gatctggaga ctctgcgtaa cgaagtatcc gtagtattac
    21121 agaagaacgt gctgttctcc ggtaccatct tagagaatct ccgttggggc gacgagaatg
    21181 ctactgaaga ggaatgcatc cgtgcctgtc gtctggcctg cgccgatgaa ttcattgaga
    21241 agatgccaga gaaatacaat acctatattg aacagggcgg aagtaacgta tccggcggac
    21301 agaagcagag actgtgtatt gccagagcac tgctgaaaaa gccgaaggta ctgattctgg
    21361 atgactctac cagtgccgtg gataccgcta ccgatgcgaa gatccgtaag gcatttgcta
    21421 cggagatccc cggtacgacg aaaatcatca ttgcacagcg tatctccagt attcaggatg
    21481 cagatcgtat catcgttatg gataacggta agatcgatgc atttgcacct cacgaagaac
    21541 tgcttcgtac taatgagatc tataaggaag tatatgaagc ccagacccag acgggtggcg
    21601 gcgactttga tgagaatgga ggtgagtcat aatgccagga cctatgggcg gcggaatgcg
    21661 tggacccggc agaagaatgc agggcggtac acgtattgag aatccgggaa aagtatttaa
    21721 acgactgatg gcgtatatct ttaaaaatta cggcattcac tttatctttg tgttggtctg
    21781 tattgtactg agcgttgtgg caagcattca gggtactctg ttcatgcaga gactgatcga
    21841 cgactatatc atgcccatga tcggacagga gactccggac tttggcaatc tgttccggga
    21901 aattgtccgt gtggctggtt tttatctggt cggtgtggcg gccacttatg cttacaaccg
    21961 tatcatggta accataactc agggaacact gaagaatctc cgtgatgatc tgttcgaaca
    22021 catggaaaag ctgccgatca agtattttga tacccattcc cacggcgata tcatgtccat
    22081 ttacaccaat gatattgata ctttacgtca gatgatcagc cagagtatcc cgcaggtatt
    22141 ttccagcttc atcaccgtag tcggtaccct gggaagtatg ctggtattaa gtgtgcctct
    22201 gacagcagtc agtctgatca tggtggcact gatgatgttc gtcactggta aggtagcggg
    22261 cttaagcgga aaatatttcg taaagcagca gaagaatatc ggtaaagtca acggttacat
    22321 tgaggaaatg gtggaaggcc agaaggtagt caaggtattc tgccatgaag ataagagtct
    22381 ggaggagttc aaggctctga atgatgaact gtgcgacagc gcatacaatg cgaataagtt
    22441 cgcaaacctg atgggaccca tcaatgccca gctgggtaac ttaagctatg tggtatgtgc
    22501 catcgtcggt ggtgccatgg cgctgggtgg tttcgcagga ctgacgctgg gtaagcttgc
    22561 cagcttctta acttttaaca agagcttcaa catgcctatc agccaggtca gccagcagct
    22621 gaacagtatc gttatggcac aggctggtgc caagagagtc ttcgatctgc tggatgagcc
    22681 ggtagagacc gacgagggat atgtgaccat cgtccgtgcc aagaaggtaa acggcaagat
    22741 cgtggagtgt accgagagaa ccggtatgtg ggcatggaag catgtacatc aggcggatgg
    22801 atctgtagat tatattgaat tacagggtga agtggtattc gacgatgtgg acttcggtta
    22861 caatgatgac aagatcgtgc tgcataacat cgagatgtat gcaaagcccg gtcagaagat
    22921 cgcattcgta ggctccaccg gtgccggcaa gaccacgatc acgaacctga tcaaccgttt
    22981 ctatgatatt caggatggta agatacgtta tgacggcatc aatatcaaca agatcaaaaa
    23041 agcggatctg cggaagtctc tgggtatcgt attgcaggat acccatttgt tcaccgctac
    23101 cgttatggac aatattcgtt tcggtaagct ggatgctaca gacgaggaag tcattgccgc
    23161 agcaaaactg gctaacgcgg atacctttat ccgtcagctt ccggatggtt acaacacagt
    23221 cctcaccggt gacggtgcga acttaagcca gggtcagaga cagctgcttt ctattgccag
    23281 agcagccgta gcggatcctc cggtactgat cctggatgag gctaccagct ctatcgatac
    23341 ccgtaccgag aaaattgtac aggacggcat ggataagttg atgaaaggaa gaaccacttt
    23401 cgtcatcgcc cacagacttt ctactgtcaa gaacagtgac tgcatcatgg ttctggaaca
    23461 gggccgcatc atcgagcgcg gcaaccacga tgaactcatc gcccagaagg gcagatatta
    23521 tcagttgtat accggtaatg cgatcgctga ggggtaagtg aatagatatg ctataccaaa
    23581 aaacagtgcc tggattggca ctgtttttta tatgaagtat tttataaaga ggacttaacc
    23641 tgagatcaat acccaggtat cccgccaacg caagtatttt attacaaaat ccacatccac
    23701 cggctgcccg gacaataccg gatagaacag caggaacagg gcaaacgcca ccgcagcata
    23761 agcgcagcag acgatgatga aggtgcggtc agacatatgc ttcttccact gcatcaggct
    23821 gtacacgatc atcagcacta caaaaggaac actggggaaa tagtggtaga tgaaggtaat
    23881 tctggtgacg aagaaccagg gcaggtactg cgccaggtag ccgactgcga ggaaagcagc
    23941 tgctgcatat tctctgtggg acagggtggt cgcagtatct gtggagcact ctgtttgtgt
    24001 gtgtcctgtc agagaacaga gaaacgcatt gttgcgaatt aacaggtaca ggatgtaaat
    24061 aaatgccaga ataccgatcc accatacaac aggattgccg aaagcactga ttccctcttt
    24121 gactgcatcg gtcagatatc cggaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24241 nnnnnaggat tgccgaaagc actgattccc tctttgactg catcggtcag atatccggaa
    24301 tagtaccaga tcgggcggac catagtgggc cactgatacc aggaagagga ataaggatgc
    24361 gttgcctcca ggccgctgtg atagttgaac atggaagtct ggttggtcag catacggtca
    24421 aaaagtcccg gatgggaatt gtctacaaat ggcaggtagg acaacagata gatcactgcc
    24481 ggaaccagca cgaaaaaggt caggcagaag tcgatcgtct ttaccgtata atccgggaat
    24541 ttctttacaa tctgctgatg ctccatgccg ttggtgcttt ttccgggatg ggctttggca
    24601 tagaggtatt cccggtagcg tcgcagcaga tgcgcaaaaa acagcagtgc cagtccacag
    24661 cccgcataga taccggtcca tttgctggca attcccagtc ccatgcagat gccgcataag
    24721 cccagaggca caaaggtctt atacaacggt gtatcgtaaa aactcataga acaatagtag
    24781 tacataaaat agtacattgc aatcacaaag aatgtgatat aaacatcgat ggtggcaatc
    24841 cttgtctgcg taaaatgcat aaaatcaaag gcaaacagga agcaggccag agctgccgca
    24901 ggagtattcc ttgtcatttt tttccccagc aggtagataa acggcagcat ggcaatgccg
    24961 aacagagtac ccatgatacg ccagccgaaa ggattcattc caaagatggc aacacccagg
    25021 gcgatgaaga tcttgcccag aggcgggtgg gtattttcgt aggtcggcag gccgtgaagg
    25081 aactcgtaag ccgtgcgtgc gtgatagatt tcatcgaaat acatgctgtt gcggaaggaa
    25141 tagcgttccg ggtacagatc gctttcgtca aacagtgtag ggtaggcatc tgcgttcaac
    25201 gggcgtgtga tgttgccatt cgcgtccagg aagaccagtt caatcagaga ggcatcatta
    25261 tccgtcaatg acagacgcag atatctggtt gtggcagaca tatcagaagt actgttttct
    25321 gtggcctgtg tcagcagatc ctgcagggag acatcctgcc aggtgaagac attgtttaaa
    25381 atgatctcac cgagataagt ccaggagtct tcggcattac ttttgacatc catcccaaaa
    25441 tgtcgctggt gccagggagc cagataactg gccatagtca caggcagcgc atcttcgggg
    25501 aattccagct cgatggcctg taattctccc gacatatcgt aagttgttgt cggagctttg
    25561 cggtctccca gatcatagag ggcaaagcaa ctgtacagaa taccgattgc cagcaacagg
    25621 cacagatccg gtttcgtgaa acggattctc tcttcggaag gaatgggctc caggggagag
    25681 agaaaatatt ctcgaagtct ttgtccgagt cctgcggcag atcttgtgga agcagtatgc
    25741 ccggaagctc tacggcctat cgtgggctgc ggctttgcat cggtggcagc tcctgcttca
    25801 ccgtgaacat aatatttcca gatgatctta tagaaataat acaggcagca caacattccg
    25861 gcagatacca gcagaatgat gggagccttg cggtcataat tctgcggatc gtaatggtac
    25921 agcacattgg cggtgttgta aaaatgcagc actgcaaatc cactgaagca tttccagaga
    25981 ggtgtagagg gccggtacag gcagcagaaa agcaacaggg ccagagcggg atacatataa
    26041 cgctcatgca tacggacgga gaacaggaac atcgtcagga tgataaaggc accgagacag
    26101 aaatatcttg aaggctcttt ccggcagcgg gctgcaataa ggaaggtaaa gagtacgatc
    26161 agcaggatca cgatagtgcc ccaggtctta cagggcagga agaggaaaat cgtgttctgg
    26221 tcgacccagt tcattcctaa tagtccccag aaattgtagg cattgatggc tgcatattca
    26281 taggatccca acgttgaagt atattgtgag attgcagcgg ttaatccgaa aggagcgcac
    26341 agaaggagca tcccgcagat gacaaccaga ccaccgcaca aattatagaa gaatttccgc
    26401 caggaaaaat cctgtaagaa cacatggtcc cagatccctg ccagcagtac cggcgtgaag
    26461 atcagcatct gtggctttaa caggacaccc agtccgtaga cagcataggc aggaagcagg
    26521 tttcccttca tgaggaacag gcacataagg atgatcatca gcgtgtatac ggaatccacc
    26581 tgtccccagc aggaagaatt cagaatgacc gccggctgga acagatacgc agaggcagca
    26641 aagattccct gcagatcgga aaaatgcagt cgctttaccg cctctctgta gagtagaaac
    26701 ccacaggcaa tatcacagag aattgcgggt aattttaaca gtaaaatatg cagatcggaa
    26761 taataaggaa tctgcagcag agatcgtaac gctccgatca gatacaatac atacatgaaa
    26821 cctggcggat agtccgtaaa catctcggca gaatagaact gccctggccc taaagtgaaa
    26881 atccggtcag cccaggctgc aaaacaggcg atatcattac tgaagccgtg ggtggtgtat
    26941 gcaaggagca ggcgcagaag cagggcacct ccaaatacaa gcaacagaag agaacgccgg
    27001 ttgcctgtat tgtggaaatg agaactgttc tgaaagcctg tatagcagac accgcacaaa
    27061 agcagtgaaa gcagtgcaaa aagatgtatc ataagatttt attcccccaa tagtttctta
    27121 tttaaagtca taccttaaaa atatcaaaaa ataactcttt cgtaaagacg aatgcggaaa
    27181 aataaggtat aatcattaac aaattataac gattcttgca ggaaggggaa actggcatga
    27241 aaaataaaaa agcggaaaat gcgatcacag cagtgctggc ggcagcggta ctgctgaccg
    27301 gaggaatgag tctgcattct tatctgtcag agcagagagc aaaaaatgaa tacgatgcca
    27361 tccgccagga ggtggtggca gagcctgcgg cgggggaaac acaggaagag attgcggaga
    27421 aaaattatcc ggagctgcag atcgactttg ccgaacttat caggaccaac caggattttc
    27481 gtggatggct ctatttcccg gcattggaca tcagttatcc ggtggtacag ggggaggaca
    27541 atgactatta tctgaagcat tccttcgagg gggagagtgt gaatgctgga tgcattttca
    27601 tggactgtgg ggcttcagca gactggtcag acaggaatac ctttgtattc ggacataata
    27661 tgcgggatga gagcatgttt ggggctttta aaaatttgct gaaggggacg gcaagctgcg
    27721 aggagaatcc ttacttctat atatacacgg aagataaagt ctacatttat gagatttttt
    27781 cctattatga aacaaagagt accagtgacc gttttgccac gtttacctca gatgcctcct
    27841 atgatgatta tgtgcagtgg gccacggaac attccctgta tgcttcagat gcagacttaa
    27901 gtgacagggg aaatatcgta tccctatcta cctgctacgg ctcatccggt acaaaaaggc
    27961 gggctctgat ccatggagtc ctgacagaaa cggctgtata ctaaggctta tgttacaaaa
    28021 atagcaaatg atactgaaaa gatcaaaaat ataataaaaa tctgaacctt ttttgaaatg
    28081 gcgccagtct taatgattgt aaagaggaac gaaaacgttt aaagacgttg aaacacgcag
    28141 tttcattgag ggagaaatca gatgagcaga tttagcaagt atgtaaggga aaaagtggta
    28201 aaggttctgg tgacagcact ggttgtaggt tctgttgcag ggaacgaagg gattgctgtg
    28261 caggcagcag gtacggattt aaatgatcca ttttcatttg tattatatac aaatgaattt
    28321 gtatcctcaa atctggacca tattgaggga aatgccgcaa ttaaagaatt aaaggtttcc
    28381 atggtaagct atgggggaaa atcttatctg ggagatcttt ctgctccgaa tcagattctt
    28441 aaatatacag cgtcaggcag aacagacgaa aaggaactga taattccgct tactaattct
    28501 gacggaacac agaactctgt tgagcgggaa gtgactggac agggttataa tattatagct
    28561 ggtgaaaata aattatatac tggaaatctt ctgaagaact gtgaggtatg ggatacagat
    28621 attgcatctg agatcagcaa tgtcctggca ggtaatatgg tacagaaaag caacgacttg
    28681 tatgctttgg cggatgaaaa taacgataca caactggtat tgaatgtaac aagtgctgag
    28741 ttaaatacca ggatggaaga ctatgacaaa gagatcactg gcgcagcttt ttatggaaaa
    28801 acagttgtgg taaatgtaag tgattccggt acagtgaata ttaccggctg gatgaatgcg
    28861 catggtgcaa aaaagcaagc attggaacat cagctgtggg cagacagaat tatttggaac
    28921 tttggcaatg caacagaagt taatgtaaat tctattttcg gatatatttt ggctccgaat
    28981 gcaactgtct ataatggcgg aactgttgtc ggaggcatca tagcggataa gtataagcag
    29041 ggtggagaga ttcactgtcc gtgcgaaccg gatgagccgg aacctacgcc tacgccgacg
    29101 gtaccggtgg taaccgagga
//
LOCUS       DAWEJW010000025        29041 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_679_length_29041_cov_45.263472, whole genome shotgun sequence.
ACCESSION   DAWEJW010000025 DAWEJW010000000
VERSION     DAWEJW010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 29041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29041
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_679_length_29041_cov_45.263472"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5046..5145
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11920..12019
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14955..15054
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16744..16843
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19051..19150
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7473 a         7329 c         6015 g         7724 t
ORIGIN      
        1 ccgttcatct cgtaggtacc gtatttctcc aacaggcttc ccagacgtgt gacaccctct
       61 ctggcattgt gaatgtaagg aagcaccaga gacacaatgt cctcctcgcc gatgccgccg
      121 gagatctctt ccacaccatc cttcgccgga acatattcca ccagtggatc cgctcccaga
      181 actctggcat tggaagtgat ggtctctgtg gccgtcatgc ccacacctgc cgcattgaca
      241 ccgtgcgctg cccagatacc ctctccgtcc agtgcattgg ggactgcggt ataacgcatg
      301 ggatcctccg gcagatcgat ctccacatgg gaaagcacgg atttgtattt tttcggctgt
      361 tcttccggat gcaccacaac gaatttcttc ggtgtgaact gccccgaagg agaatcgtca
      421 ttccgggcga tcatggtgga accgtcataa gatgcttttt ttcctactaa tatggttgta
      481 catggcataa tcattacctc ctgatactgt gtttcaaaat tttcttctcc ggcaccggaa
      541 atctgtgttc cagtcctggc acttatgcca gcttctgtcc gttaatgact acattttgaa
      601 ttgtctccgg cctgccctgt aaaggctcga tctttgtgaa ctgtctgcag ccggtgactt
      661 caatattctc catctgcaga tttctgaaat acgggatgtc gtcctcattt tccatggcaa
      721 tggtctcttc ccggttcagg gaattgagca cataggacat ggtcaggatc actgcctcgc
      781 ccttgatctc gctcatatgg atattgcgga gagtgatatt ttccaccacg ccgccgcggc
      841 ccagcgcact cttcatgcgg acacccacat cggttcccac gaaagtacag ttctccacca
      901 gaatatcctt tactccgcgg gacatctcac tgccgatgac gaagccgcca tggccttcgt
      961 tcaccagaca gtcatggatg taaacattgc tgcagggccc ctgaatcttg cgggcaatgg
     1021 catttttgcc ggacttcatg cagatggcat catctccggt ctcaaaaaca ctgtgatgaa
     1081 tatgcacatt agtgcaggac tccacatcaa tgccgtcacc gttctgggca taataaggat
     1141 tgcggacctg tatatgctcc accgtaaggt tctcgcagaa ataggggtgg atattccatg
     1201 ccggagagtt catgaacgtt acgccttgca gaagcacatt tttgcaatgg cgcagactga
     1261 tcatcaccgg gcggtaaaaa tcataatagg gtgccgcggc ggccagtgct tcctcggtgt
     1321 ttccctgaat gttgtggtga tttcctttta aaatcgactc tgtgggcatc cagatcgctg
     1381 tttctttcgt ctctaacaca taagggctga tggtaaacaa cgtatcccac tgtttttccg
     1441 tcatcttaaa cttcttgatc ggacgccatt tatcaccgct gccgtcaatg ataccatctc
     1501 cggtgatggc gatattttca gcattttccg ctgtgatggg agacacggta cggatgcagt
     1561 cctgcccttc ataactggta atgcgcaggg gataatcctc tttggacttg atgaatttta
     1621 acatagcctg actgtccaga tgaagattga caccgcttaa cagccggagc ggagccaccg
     1681 cccagatgcc tgcgggaact cttaccgtac cgcccccctg ctccgacact tcgcagatcg
     1741 cgcggttaat ggcctccgca ttgtgtctgc cggtctcgat ctgcaaatcg ccgctcttcg
     1801 cgccgtattc tgctatggac acctcccggt ccgggaactg gggaagttct actttataat
     1861 tctgattata catggttatt gttttctcct ttgaatattt tcatggatac tttcacattt
     1921 tctatgtttc tgattctagc gtgttccgtt tcggacttcc acccggtttt tgcgtttttc
     1981 cgaagggttc tactgcttat ttgtgccctt ccgcgcagat ccagtcctcg ccgtctttta
     2041 agtgaatccg tacctcttca aatcctgcat tttccagcag ttcccggaat tcttccggtg
     2101 tgggattaaa cagatggaat ctttgaatgt tttcccgcac ctgttccggc agatcctcct
     2161 tctggtaaat gtcggccacc agtaaaaagg ttccctgctc cttcaatacc cggtagactt
     2221 ctttcagatt ctccgcggga tccggccaga aataaaagct ttccaccgta atgatcttgt
     2281 caaaagcatt gtccgcaaag ggcagttttt ccactgaggc ttccacgaga tccatttttc
     2341 cctgctgcag tgcttctgca ttggttttcc cggaaagctc cagtgaagtg gcggaataat
     2401 ccactccggt cagatgtccg tcacgcacct cttcactcat gcgtttcaga gttgctcctc
     2461 caccgcagcc gatgtccaga atccggtcat tttcccggat cttccagtgt tccaacgccc
     2521 agccggtcac tgcgtagtgg ctttcgttca tgcggcgcag catcttctcc ccatcctccc
     2581 cgaccggttt cccggggttg ccggagatgg tgatctcctg ctgggtgcgt tcgttggtgg
     2641 ttaggtttat ggtattattt tctgtttttt cagtcatgat ctttattcct tcttccagcc
     2701 gctttctttt tggatctttg cggttttctt tgcaattttt actatctcta ttataatagt
     2761 aactgtttat aatcatgtaa agggcaatcc cgttattgag aatatctctg cataagggat
     2821 ttggcaaaac acccgataca aagtacaatc tgcgaaagca tacgccggaa atgcaatgga
     2881 acgcttgcag gttacggaaa ggcatacatt tttatgaaat attatcttgc ccccatggag
     2941 ggactgacca cttataattt tcgcactaac tggaaccgct gttacggcgg catggacaaa
     3001 tattttaccc cttttatctc caatcggcac atgaacagca gagaacgcaa cgatgtgctt
     3061 ccggaacata atgtgggcat gtacaccgta ccgcagatcc tgaccaataa ggcggaggaa
     3121 tttctgtcgc tggcagagca gctggccgga tatggctacc acgaggtcaa tctgaatctg
     3181 ggctgtcctt ccgggacggt ggtggcaaga aaccgtggtg ccggcttctt aggcacgccc
     3241 cgggaactgg aggattttct ggacgagatc tttgaaaaat gtccactgga aatctccatc
     3301 aagacccgca tcggcatgga ctacatggat gaatgggctc tactgcttaa aatatatcaa
     3361 aaattcccca tgaaggaact gatcattcac cccagactgc aaaaggaagg ttacaagggg
     3421 acaccccatc tggaagcttt cgcagaggcg gtggaagccc tgcaatgtcc gctgtgctat
     3481 aacggggata ttacctctcc ggagtcgcta acgcagattc ttacgaagct tcccagtatc
     3541 gatatggtca tgatcggccg gggaattttg cagaatccgg gattattgca ggaattgaag
     3601 gctgcctcca cggagagtgt tgcgctgccg tcttgtgaag agcgtctggc tgtcctgaag
     3661 gaattccaca cttccctcct gaccggttat caggagatca tgtccggcga taccaatacg
     3721 ctctataaga tgaaggatct ctggactttc ttaagccaca gcttcgaatc tccggacaaa
     3781 tacgtaaaaa agatccgcaa agccacagat gccagcgact ataagctggc cgtcaatgcg
     3841 ttattccggg agtgcgcatt gaagtcggag aattcttgat cctgacatca tcccatacct
     3901 ttattccccg gtgtcgaacc gttctccttc gttggcgtct tccatgacga cctccggctc
     3961 cgggatgcct gcattttcca catagctgaa atgaatgacc gcctgtggcc agttgagact
     4021 gatccgggcg gtcttctgcc gctcctcgcc tgccttgcgg atcagttcca acagttcctg
     4081 caggacttct ctggtcagat agcctccccg ccactggaac gtcatggccc ggcctttttt
     4141 cgccatctgc tgtgctcttt tatatacttc ttccgttttc gtaaaggaaa gttttttctc
     4201 cttgtaataa aaatgatcgt tgtccggaca gccgggtgcc ggggcgatca acggttcatg
     4261 atcccggaac actgctttat ctcccagatt gaaatagtcg tagcggatct ctccgggtgc
     4321 ttccgcattg ccctgatgat ttcccaatgt attgtcaaat gtggcatcca gatggtaata
     4381 ctgcccgccg atccgcacaa tattccaggt gtgacggtac ttgatccctt tctccggatt
     4441 gttgccgcag atagcgatta cacaccacac acccagcgca tcacaaagca ccttcaccga
     4501 tttggcaatg ccctcgcaga cacccacgcc ctgtcccagc ggtccgataa tctcatggga
     4561 atagggcttt ttcaacttat cgtaggtgat attttcgcag ataaagtcgt gaatgtactt
     4621 ctctttttcc cactctgtga actttgcggc aggtcttgcc agcttttcca cccgggactg
     4681 catggctttc tgatgttctc tgatcttatt tttctcaaac agatactccg gcacgaagat
     4741 gatgttgggg gagtcctgat aatatttata ccgataactc accacccaga agatctccgg
     4801 atgatccagc cgcagtcgga aaaatacgtc atacaattct tccgccggga tccgcggcag
     4861 ctggatctcg tctgccagcg ccagcactcc ctgctgcatg gcgtgatagg cggcctgctg
     4921 gggcttgttc attttctgat agtaatatgg ttccatggta gtttcctatt ctattgtttt
     4981 catctgtctt atttttacat tttaacagaa ggtttttctt ttgggtggat gtttttgata
     5041 tttttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatttt tacattttaa
     5161 cagaaggttt ttcttttggg tggatgtttt tgatattttt gatatttttt gatatttttt
     5221 tgatattttt tgaaactttt gcagcagatg catagtctaa ccatcagaga cctgtaagat
     5281 atagttttat ttaagagagg agttttactt atgaaaaaca aaatgatctg tgcactttta
     5341 actactgtga tggtattgtc ttttgctgcc tgtggcagtc agggaaatgg agcagctgcc
     5401 tctactgaaa gcaccgtagc ttccgaaagc caggttacag aaagcaatgc agaagccaat
     5461 actgaaaccg catccaccga aagcacctct acggcggaag gagcctatga agacaacttt
     5521 gcagttccca cagaggatgc tgccgctttc gctaaaaaga tccaggatgc cgtagccgct
     5581 gaagacctgg atgcactcgc tgatctggtc aattatccgg tctatgttgc tctgggcgat
     5641 ggcagtgtga tcgaaaccag ggaagatctg attgccttag gtgcagacaa aatcttcacc
     5701 cctgagctga aagactctat ggctaatgct gatctgtccg aattaagccc cagtatggca
     5761 ggcttcaccc tctacagcac cggtgacggc cccaacatca ccttcaatgt ccagaacgga
     5821 gtactcggca tcagcggtat caattactaa tttccgcttt gaaccattaa ggggagcctt
     5881 tcatcaggct ccccttactc gtgtcgaaac aaccgttgtc aacggtacca tagtgatatc
     5941 tatgttcccg tttgtcttac gcgtgatcag cgtggctccc cgctggatat attgcttcgc
     6001 gatgccggaa tatcccagat aatcaatgga cattccgtaa gtcatgcgga tacctttata
     6061 ggtcagtgac aacgtattca gatgatcgtg tccacagaaa atccatttca gccagccgtt
     6121 atccaccgct ttttcaaaaa aatgaggtgg tcggttcgat attccgaagt actcgtcctt
     6181 ttctccgata ctgccgtgtt cataaatcac gctgtgatcg cccaatttca ttttctcata
     6241 agcttcctta aattctgcgg gcggcatgtg gaaaaatgcc atggcaggca cttttctctc
     6301 gttcagtttt tccatgcacc agtccgtctg atcctcatgg atccggtcga aaccgctgaa
     6361 aaaccagccg tcaccataca tgttggaatc cagcagaatc agcggaagta acagttttcc
     6421 ctgatcatct gtgaggtcta ctacaaaatt ccctacgccg gtaatgggat tctgaggact
     6481 gtgttcatat cttcctttgg taaaaacagc gtatttcccc gtggcaaaaa tctccgccag
     6541 ctgctcctta ttgcaggtgg agcccatttc acagtcatgg ttaccaaaga ccagagtata
     6601 gggaatctga aaaccatcca gaaatgccag cagtttttct gcctgctttc ggttgttcat
     6661 ggtaccgctt ttcgggaaaa acgggaaaac agaatctccg gtcaggacga tcagatccgg
     6721 tcccgtccgg cggatcagct ccgtcaccgc ttccagcgcc agtctgtcct ttttccggga
     6781 aaacatgccg aaaccaaggt gcaggtctgt cagctgcaat attttataat ctctgtccgt
     6841 cgggatgcaa agagaaaaag tctcttcgtc ccgaatctga aagtttttgg aagtatacat
     6901 aagaatctct tttctttttg gggattaact caacgggatc aatgctccta ttgcagaaag
     6961 tactgttgaa atcaccatca gcacctgaaa aggcaccgtg ccgatcacac cgcagatagg
     7021 actgctgccc gccttttttc tggatttacc gtatgtcagc accagcatga tgctcaccag
     7081 taccacaaca ccgctcataa tccgggtcag aacgataaag ctgttcacgc caaatacggc
     7141 aaacaaaatg cagggaactg tggcgatcag ccagctgatg cggttgccgc tgccgatctg
     7201 ctcatgaacc acatcgcgga gggcgagagt attgctccag aacgtggtaa gtaaagcaaa
     7261 cagggaaaat acgcctgcca ggatcatcgc ccaggtaccg atactttctc ccaactgcat
     7321 gtatgccagt tctttttcca gcgctccctt ggttcccagg agaaccgtgc ccgttacgat
     7381 caggacaaac actgaggaaa gtccaaaacc gatgaaaatg gagcggcgga tctgtttcgc
     7441 gttgccatcc agacctttca ccacctgaat caccgcctga ttggcggaag ttgcgaatac
     7501 caccatgctg tacagcgcca acaggttgtt ccagtggaac tctacccgtt gcaatgtatt
     7561 cagcggatgc agaaaagtcc ccaccgtcat caccgcaacg attcccatca ggacgatgac
     7621 tgcgtatttc tgcgcgattc ctactgcttt catgccgata aacaccacga ctccggcgat
     7681 cacatagtac aaaatctgcg ccgccacgaa gggcatcccg aaccagttca caaatatctg
     7741 ggcgccaccg ttgatattac cgctgacacc gatcataatt gccagcccgt agacaataaa
     7801 tacgccccag ctgaatatct gtttcatttt gccgtggaac agctctcctt cgaaactttt
     7861 taccagctgg acgccaccgt tgttatagga caactcggca atgatcaggt gcagaatcac
     7921 gttcacacaa tatgcaatca ataccatcca tacgacatcc agcatactgt tcttcgtggc
     7981 aagatacgga acggcgatga tgccggtacc gatgctgttg ccgacgatca tgctgatggc
     8041 ttcgaaggtc gtgagatggg cttcggattg gatggggtgg gacatgggga ggacctcctt
     8101 ggtaaaaata ctgattttct atagttataa aagtttagcg gtaagtgctt cctgcaatac
     8161 tttggagcaa ttgattcctc gcttatcggc aagtttggac atccatgccg gaagcgagac
     8221 atttttgcga actgccttgg tgtcagtcat gctacgatat tttatagtgt ctgcctgaat
     8281 gatcgtaaga atatctgtcg gtaaatgagg aatctctgac tgcggtgtag gctcattcgg
     8341 agtaatttcc tcatcttcca gaccaacaag acaaagattc aatgcatctg taataagggc
     8401 aatcgcctct gccaaagtcg gtgctgtagt aatacacccg tccacatccg gaacctttgc
     8461 atagaaagtg ccatcttttt cagtaataac tgcggtatat gtgtattgca tgtcatttgc
     8521 ctccttctat atttgcttct ttgaagatat agcgcatatc attttcatta aaatcatgac
     8581 gttttacagg aattgtctgc ttggtattcg gttgaaaata caaatcatga cttgctccat
     8641 gtctttaaaa aataaaaccg ttcttttcca attctttaat tgttttcttt cgtggatgca
     8701 ttcttttctc cttttatatt atacacaatt atacataaaa tcaataataa aatacacaat
     8761 tttacacaaa tgattatgtt tcatttattg catcaaaaat caaattgtta catgggaaat
     8821 ataaatcgat tcttttaaaa ctgactttat aagatatcgt aattatcaac acactgtacg
     8881 atatcagaca taagatagaa taatataaga aaatatcaca atctatagaa actgtccggc
     8941 aggcgtacca ttctcctgca tctctcgggt ttcccctgtc aagccaacgg cgtgtggacg
     9001 ggtacgaaat tttccgcctc gaacagtttc cttgattatt tgtaataata acatccttag
     9061 agttcaatgt aaaggtactt aaagttcttt taatttaatc atttttagtt taattatatc
     9121 atttgttgaa aaatgatata attgaatcaa ctatccctta tgttacggca gcatcccctt
     9181 gatcttcacg ccactcagat ccacatccag cacgatcttc accatccaga agaacaacag
     9241 cagcaccagc atcgggatca cgcaggaagt aatgatcatc acggcaaccg cctcgataaa
     9301 gtgatttagg gaattctcca ccttctgtac cagatcttcg gtagaaaccg tcacattttc
     9361 cacgacattt gccactgaat ctttagcgcc ggaaatggca tcttttgccc agtcgaaaat
     9421 gtttgagaca gagccggagt tattgttgtc cttctcctgc acatttcctg taacagttcc
     9481 agaagcttct gttgttccga ttccggttgt ttccgtcgca ttagtgtcat ccaccacgcc
     9541 actgttttcc agtatattct gtgtgttcct ggcatcctca atggtagctt cgatctgggc
     9601 ctgataggtg tctccgataa ggtcagaaat ctttacactg gccggaacca cgagaaaaat
     9661 ggcaatgcca aatactgcaa gttttgccgc cagtttccgc accgtagcat tttcaaaagc
     9721 cagattcaat atccataatc cacaggcaat cggaataagg tatgtaaatg ccacatatcc
     9781 ggtaatcgtc actagatatt tttcaagata aatggcgcag agaaccacca gcagataccc
     9841 gctgacatcc gccattttct ccgcaatggg cgttgccgtg tctcccggaa gcaatgtgat
     9901 cagcgccgaa gtcaccgtgg atgccgccgt cagctccatg accgtctgtt ttttctcatc
     9961 cagagaggct atggttttct ggtgattttc cggggcggag acgtattttc ctacaaccgt
    10021 tatggacaaa atagcaacca gcaataatac tgctgctgta atgcatttct gtttgataaa
    10081 gttcatacta attaccttct ttccttcacg atttctatat tatttcacgt aaatatcctg
    10141 ctattacatt tttcaggacg taatttaggc tatacactac acttttcagg acgtaatctc
    10201 gtgccatctc acacttttca ggacgttact tcaaaaaccg ccgcaacaac ttctcttttt
    10261 tctcatcata cctctggtac cgtatcggca gatccatcca tgtctttttg tccacgatac
    10321 tccgataatg gctgaaggtc tcgaatccgg tgcggccgtg atacccaccc atgccgctct
    10381 cacctacgcc gccgaagggc atggcactgg tggcgagatg aatgatggtg tcgttgatgc
    10441 agcctccgcc aaaatggcaa tagttcagga tccgcctctg ggcctttttg tcttcgctga
    10501 aaaaatacag agccagagga tgaggatgtt cctccacaca gcggatcgcc caatccacaa
    10561 agtctcccgt ggctgcatgc gtcccggaag catccctgca aaaatcattt tgtgcaacac
    10621 ctttctctgc atcatgtgca ttatacgtca gcaccggcag gatcggtccg aagatttctt
    10681 ctcccatcac ggcatcttcc caggtcacat ccgtcatcac tgtgggagca atccgcaaag
    10741 tcttctcgtc atactgtccg ccgcagacga ttttttccgc atccatcaat cccaacagtc
    10801 tgtgaaaatg cttctcgtta atgattttcc cgtaatccgg attctgcagg ggatctgcac
    10861 caaactgcct cacgatctcc gcccggatag cttccaccaa tcggtcgcgg atcctgacat
    10921 cacacaacac ataatccggt gccacacagg tctgcccaca gtttagatat tttccgaata
    10981 cgatcctcct cgccgccagc ggaagcttcg ccgtagcatc cacgatacag ggacttttcc
    11041 cgcccagctc cagcgtcacc ggggtcaaat attccgcagc acttctcagc acctcccgtc
    11101 ccacggtctt gcctccggtg aaaaatatca tatcaaaccg ctgctgtaac aatgcctgat
    11161 tctctgctct gccgccggtg atcaccatca catattccat cgggaagcat tcttccaaaa
    11221 tcgtttttaa tactgctgcc gtcgccgggg cataggcact gggttttacc actgcggtat
    11281 tccctgcggc aatagcatcg atcaacggct ccagtgtcaa tagcaccgga taattccatg
    11341 gactcatgat cagcacggta ccatagggtg acggagaacg gaagctcctc gccgcaaact
    11401 gtgataacgg tgtcggcact ctcttttccc gcatcagtcc ccgcaggtgc ttctgcatcc
    11461 aggtcagttc cgacagagtc agtccgatct cgcacatata gctctccatc cggctttttc
    11521 ccagatcctg ttgcagtgca gcattcagtt cctcttcatg gtgctgcagg gaggacttta
    11581 attttcccag ctgttctgcc cgaaatttcc gggaaagtat ggctcccgtg gcaaaatatt
    11641 ctctttgtct gttcaatatc gtccggatct cctgctctgt catagatgcc tcctgatttc
    11701 tctgctcttc tattctttat actacccatt ttttcatttc aatgcaacga aaaacggcgg
    11761 agcttttcac tccgccgccc gcctatttct atttacttta tttccacgca aaatttacca
    11821 ttccgtacac atagatgaac aacactgccg ccggcacaaa atatttgaac accggcttca
    11881 tccaatgctg cactttcaat ccttttccct cgttggcttn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnt tgaacaccgg cttcatccaa tgctgcactt tcaatccttt
    12061 tccctcgttg gcttcctcca ggaatttgtc ccagccccag ccgaccttat aacagcaaaa
    12121 tactgccagc aggatcgaac ctatgggcat gatattgttg gacaccagga aatcccagaa
    12181 gtccaaccag gtcgttcccg ccgcaaaggg ctgaaaatgg atcacgctga agcccaacgc
    12241 cgttgtcaat gccaacagaa acgttcccac accgcagacg atacatccta ccggtctcga
    12301 ccatccggtc agttcccgga ccatggccag aatattttca cacacgccca gcactgtgga
    12361 catggctgcg aacaccatga acaggaaaaa taaggttccc caccatctgc cgcctgccat
    12421 gttgttaaat actgtcgcca tagtatcgaa cagaaggctg ggacccgccg tcacttccac
    12481 atcataagta aagcatgccg gaaatataat gataccggca atcactgcca ccagtgtatc
    12541 caggataata atatgaatag actcccccat gagagaacgg tccttgccga tataacttcc
    12601 gaagatcgcc ataccgccca ttcccacgga caaggtgaaa aatgcctgat tcatggcgcc
    12661 caccaccaca gaaccggtga ttttggaaaa atgagggatc agatagaagc tcaatccctc
    12721 tcctgctccc ggaagtgtaa agctgtgcac cgccagcacc aacatcagta cgatcaatgc
    12781 tgacatcata tatttggtaa tacgctccag cccaccctgc agatggaaac ttaaaatgac
    12841 aaaagcgatc agtaaggtga acaacagata tgtcacattc accgagggcg tcgtgatcat
    12901 tttttcaaat ccgaaatcct gattctgccc tgtcagaaat tttacaaaat aatagaggat
    12961 ccatccggac accaccgcat agaaagcgat cagcgcaatg tttccgatga gacagacgat
    13021 gccccacaga ttccatttgc ccctgccgcc ggataatttc cgatacatat aaagtggact
    13081 cgtctgcgcc gcacgcccca cggagaattc catgatcatg gtgggcaggc ccagcacgat
    13141 caggcagatc acgtagatca gcatgaagct tccgccaccg ttctgcccgc acatccacgg
    13201 gaatttccac acatttccgc agccgatggc acaccccgcc gacagcatga taaatcccaa
    13261 acgactcccc agtctttctc tgttttccat acccatgatc cttcccaaaa ctacttattc
    13321 taaacatggg atacgacata tatttttgct gttttcccat atttccgcca tcttttcaca
    13381 ttgacagcct tattttttca ttatataatt ttcataaata tttgaaaaac aaattatttt
    13441 tatcatattg atcgaataat tcaatcgaga aggtgcaatt attttatgga tgtcaaaaat
    13501 ctggaaacct ttgttatggt gaacgaactg aagagcttta cactggcggc acagcgcctg
    13561 ggctacaccc agtccaccgt gtcttttcag atcaaacagc tggaaaaaga gttgtcgatc
    13621 ccactgttcg agcgcatcgg tcataccgta accctgacca acgaagggga gaagctgtta
    13681 cccctggccc agcagattct gcggcttgct gcggaagcca cccacatcag cggtaatacc
    13741 gccccggagg gtctggtgcg gatcgccatt gcagagtccc tggccagctg ggaatttcac
    13801 agccgtttta aggattttca tgaaaaatat ccgggcatcc gcctgaagat cattgctacc
    13861 agtactgacg aaatgttcca gatgctgggg cagaatcaag tggacattgt atacacgctg
    13921 gaccgccgta tttttaatca caattatgtg accgctttcg aagctccagt ggatattcat
    13981 tttgtcaccg gtgccggtca ccccttagcc ggacagactc agatcaccct ggaacagatt
    14041 ttggaatatc cgctgatcct gacggagaaa aacatgagtt accgggctgc tctggatgat
    14101 ctgctctccc aaaacggtaa ggagacaatg cccatcatgg aggtgggcga tacccagctg
    14161 atccgcaatc ttctgtgtca gggtctcggt atctcctatc tgccggagtt cgttgtagcg
    14221 gacgatctgg caaaaggaaa tctcgtcagc ctccccatga cggagattca gccggatatc
    14281 tggcggcagc ttttatatca taggaacaaa tggatctcac cggaactgaa atgtgtaata
    14341 aaatacctgc aggaggaata atcccctgca ggttttctat tatatatgga tgtcgggggc
    14401 aaaagcccca cctttaattc ctacgaattg tttcgtgctg cacactccca cacttctatc
    14461 caatattcgc ttatcgagga atcttcatcc cagaagcatt ccgattccat gtaccaaaaa
    14521 ggctgccacc catgccacaa aacactgcca gagtaccacg gcaatggccc attttccacc
    14581 cagttctctg cgtacagaag cgatggctgc cacgcagggc gtatacagca ggctgaatac
    14641 cagtaatgta gcggctgcca acggggtaag taccgtggca atactgctgc cgaacaatac
    14701 ttccagggtt gctaccacac tttcttttgc catgaatccg ctgatcagag acgtacagat
    14761 ccgccagtct cccaatccca gcggccggaa cgccggaacc aacagacctg atagcattgc
    14821 cagcatactg ttggctgaat tctctaccag attcagtttc agatcaaaac tctgtaaaaa
    14881 ccatactaca atcgttgcaa tgaagatcac agtaaatgcc ttctgtaaaa aatctttcgc
    14941 cttttcccac aacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccatg
    15061 actgccggta ccgtacatcc gaacccaatc agcatcggca caatacttcg tccggacaga
    15121 ccgatctttc gaagcaactt atccatcacg aatgccacac gggcgatata accgctgtct
    15181 tccataagtg aaaggaagaa aaacaaggtc accacaatag gcaggaaact caatacgctg
    15241 cccaccccgg taaaaatacc gtcgatcaca agactttgta cggcactgtt gacatgggcc
    15301 gctgtcatgg ctgcatccgc aagttctgtc agtctgccga ttcccagttc caaaagccct
    15361 tgcagccagg ctccgatcac attaaatgtc aaataaaaca ccaatatcat gatcacaata
    15421 aaacaaggga ttgccgtata ttttccggta aggatccgat cgatcttctc actgcggatc
    15481 cgttctttgc tttccttcgg ctttactacc gtctgttcac ataatctctc gataaaatca
    15541 aaccgcatgt ccgcgatagc cgcactccgg tccacaccac gctctgtctc catctggcag
    15601 acaatatgct ccagcatttc tttttcattt tcatccagct gcagcttctc caggatcaga
    15661 tgatctcctt caatggcttt gttggctgcg aaacgcaggg gcagctttgc tgcttccgca
    15721 tgatcctcga tcagatgccc caccgcatgg atgcagcggt gtacggaacc atcatgatcg
    15781 tttttatcac agaaatcctg ccgtagcggc cgttcctgat attttgcgac atggactgca
    15841 tggcggatca gttcgtccac accttcgttt ttcgcagcag aaatggggat tacgggtact
    15901 cccaggaatg cctccatggc attcacatcg atggaaccgt gattgccggt gacttcatcc
    15961 atcatgttta gggctacgac catgggaatg ttcatttcca acaactgcat ggtcagatac
    16021 agattacgct cgatattcgt cgcatccacg atattaatga tagctctggg tttctcatac
    16081 aggataaaat tacgggatac gatctcttca ctgctgtaag gggacatgga ataaataccg
    16141 ggcaggtctg tcaccaaagt atccggatac cctttgatgg aaccgtcctt acggtctacg
    16201 gtaactccgg ggaaatttcc cacatgctga ttcgaaccgg tgagctgatt gaacagtgta
    16261 gtcttgccgc agttctgatt tcccgccagg gcgaacgtta atgtcgttcc ctccggcaaa
    16321 ggattctcat ctttttttga atggtatttt ccatcctcac ctaatcccgg gtgctccgca
    16381 tccgtcaccc ggtccagccc tttatgttct cttgttctct tcgatatagg tgcgatctcg
    16441 atctgatccg cttccgccaa acgcaacgtt agttcatagc cgtgcacctg caattccatg
    16501 gggtcaccca tcggagccag ctttaccacc gtcacctccg ctcccgggat catacccata
    16561 tccaggaaat gctgtctgag ttctccggaa ccaccgacgg tttcgatcac ggcgctttta
    16621 ccgatttcca attctttcag tgtcataaca cttctgtatc ctttcgtatc tcaacatttt
    16681 aatgttctgt gttgtaacag aagtattata gcgcatttgg tattgaaaat cagtatcaat
    16741 ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaataag gcgacacgac
    16861 tattaaaagt cttcgctacc atcgagatcg gcattgagta tctgttagtc ggtttacaga
    16921 cagatgcaag tttcattcac tatgcgctga ttgccatttt catattacag attccttatt
    16981 atgagaaaaa atctctgaag aaaaccgctg tgggattatt cgtgctctac ctgatcgtca
    17041 tgatcgttca ggctacgaag ggaatctacg gtcaggatgt aaatgccgtc tgctctaccc
    17101 tgctggtatt cctgatcgga attattattc tggagaccgg taagatcaca atacttttta
    17161 ataatgacgc catcggttct tcccgggaag aacataacca tgtaaaagaa gtactggata
    17221 atattctgga cgtatcccag accgtacatc aggagaccac caaaaccaca gatcttatga
    17281 accagctggt acaaactacg gaatctgtag ccaacagtat gcaggagatc tcctccgcca
    17341 ccaatatgac tgccaccagc atcgaagaac aaagccagat gaccggcacg attcagcgtg
    17401 ctatcgagca gaccagtcat atctccgaac agatggttca gattgccgag gattccaacg
    17461 aaagtatccg taaaaacatg gctgccatgg aagacttaaa acaacagtcc gctatgatca
    17521 aagataccaa ccattccgtc acggatgcca tggaaaagtt acaggaaaag acgaaagaag
    17581 tggaagagat cgccggaagc atcatggcaa tctccggtca gaccaatctt ctggcattga
    17641 acgcttccat tgaaagtgcc cgtgccgggg aggccggacg cggtttcgcc gttgttgccc
    17701 agcaaatacg gcagttggca gaacagacca agagctttac cgaagagatt acgaagatca
    17761 ccaatgagct gaatgccaat gccaacatgg tagtcaacac gatcagcggc tccattgaag
    17821 ctaccgaaca gcagggcgaa aagatcctgg ctgcttccga gactttcgaa cagctgaaca
    17881 aaaatatgga agcactggct tcccaggtgg aagaagtaaa tcaccacatt ctgggattgt
    17941 cagaatccaa taataagatt atggaaaaca tctcccagct gtccgctgta actgaagaag
    18001 ttaccgccaa tgccgagcag gtgcacacca tgagccagaa caatctggat tatgcagaac
    18061 aagtaaaaac ggcagtagaa catatccgct ctaccaccga tgagatgggg acgcaggagg
    18121 gttaaccctc ctgcgttctt tgatcttact atttcaattt caaaccgtct ttaaaagcat
    18181 ttcctacttt atctcccatt gcaagataag tattattcac aggatcatag ctgattggct
    18241 tcaatttctt cggatcgatc ttgccatccg tcaggatgct ttcgtccgca ttgacgttga
    18301 cgatatcgcc caccagaatg ccgtcttcaa aacttttcac cttacattcc agcgccatgg
    18361 gaagttcatc gatcaacggt gcattgacaa aatcactctt ggctgcatgg aaaccggact
    18421 ttgagaattt atcaggtact ttcttcgcag attccatgcc cacatagtcg caggctacca
    18481 cggtatcttc ggttccgata cttacggtaa atgctccggt ctttgccaga ttctccgtgg
    18541 tcttatgctc tgacatactg atggagatct cattcatgtc cgtaatgatt ccccatgctg
    18601 cgttcatggc atcgggtact ccattttcgt cataagttgc aatgatcagc accggcatgg
    18661 gatacattac cggctttgct ccaaaattta ctctgctcat attcgtcctc cttgtactaa
    18721 attaattttt ccttccattc tgcttcccgg aatccggtca acaccagatc cccgtccaca
    18781 atcagcggtc tctttaccag cattccgtcc gtggccagca gccgaagttg ttcctcttcc
    18841 gtcatgttcg gaagcttgtc ctctcaatta aatctgcctt gaaacaaaat gtaccgtttc
    18901 ttttctctgc tcaagtatgt aaggtatcag ttccaatgga tataccttcg tgtccttcct
    18961 tagttcctcc agcggaatcc agcagaaatc cagattgatt ctctcatgat ccaattcatc
    19021 atatccatga aacaccccat ccataggaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19141 nnnnnnnnnn ggaaaataca aaatcatcgt ttttaaatag aatgtccatg tgatctccct
    19201 tcacgtcaga tatactcatc ctgttttacc taaactttaa aatcaaaact gttattccca
    19261 cggaaatcat cataagccca agcaaaatgg ccagcgcaat aacctttgca tttttctcca
    19321 taaatcggat aggtgtccca tacttttcat caatttttgc atctcttcgc tcattcctga
    19381 tattctgctc ccactccaga tccttcagtt tcatatattc gtcataatgg cgtttttcca
    19441 gacggtcttc ctcttcgcgt accaataatt ccaggttttc tttataagaa gccccacaat
    19501 tactacaccc cttttcgtct gtgctttttc cccttatctt attatcacag tattcacagg
    19561 taaattcata atcacttgca ggtttataaa ccggaggctc gccgatttca aaattgtagt
    19621 atttatgacg tatcagctta tatttttgaa ttgcctgcat tgtagtactt atatattccg
    19681 tttcctgtgt aatggcttct tcctgggatt tcatatactc taaatacctt tgatgcatgg
    19741 cccgcttctt taactgatac tccttgttct ttccaaaatt accgccacag gttggacatt
    19801 tttcatccgc agaagatacc ttggatccgc aaaactcaca ggtaaattca aaattctcct
    19861 gaaaatgatc ctttttgtag gaaatgtact taaccggttt caggtcctta cgtgctctaa
    19921 tgcccgaaaa gaaaaacaag tatataagta ataacaggcc aaaggtaatt gctatattaa
    19981 aaaatactgt tgaaaacaaa ctgaaaaatt ccataataat gtgtcattcc tctttcgtat
    20041 taaatataat tcttaactaa tcatatcata gtaaatccta tttttcatct aaatcctaag
    20101 aatgtactga ttacaactct tgatacttca tgcatgggaa atatcttaga aaaatccagt
    20161 tgccatatct ccattttctg ctacaatttg atcataatag ttgcgaagca acgttaagca
    20221 cctgaggtgc gaatcatgat atattatgat tataccaatg taaaaaatga ggataacaca
    20281 aatgagaaaa acaattcttc ccgtattaat gacagcaaca atttctttga gcctattggc
    20341 ctgcggatca aacaattccg gtgaatctga atccaccacc aatattacag aagcagaaac
    20401 aacggccatc gtcgcagagt cagaaaacgc atctactgcc acagaaccgg atgccgggca
    20461 gagtgaagcg aacgcagaaa ccgaacgcac tgaaaccaca gaagtgataa cggaagattc
    20521 cacagaaact ccggcaccgg tggaagtatc aggaagcacg gagaatagca ccattttgcc
    20581 tctctcttat gaggatttta atgttaatgg atttggatat agcaattacg tagacttata
    20641 tggagccgga aacggctatg aagatgtaga acgtatttct attatgacta caaattgtac
    20701 ggcatatact gaataccccg atcaaatgtc atcagacaca gaaacttcgc gtggtataaa
    20761 attaggcgat tccagggaac aagtgattgc tgcttatgga gagcctcaaa aagaggtagt
    20821 gtataaaaat aaggcctata atcatgatgg tctggaaagc caatacaatt tatgctattc
    20881 ctatattgac gaaagctacc cggaagtctt tttcaattta tccttcagta tggatcaaaa
    20941 tgataatgtg ctggatattt gctatgaagc aatattgatg aaatggtata aataattagt
    21001 cgtttaattg ttttatcgtt atacaatatt tttctttgaa aaacatctac ttatcgaaca
    21061 tgatgcacaa taaacgcacg cgtgcgttta ttgtgcgttt attaaatcaa ttcataatgt
    21121 gctgaccgtc catttccaac aagcttaatc ctacccttat cagacatctt tttcaacaaa
    21181 cgataagctt ctgtattagt tccataatcc atacttgtct ccatagctac aacatgtccg
    21241 ccaataatag cataatcatc atttttaaat aaaatatcca tttaaccacc taccaatcat
    21301 taccttgaaa tacatatcat atttatacat tactccgcac acaaatagag ccaaactaga
    21361 gccatttgat aaaacaaatg ccggaagccc gcataaaggc aggctcacat aaggatactt
    21421 ttaaaaagtg accgattgag agcagcacaa cgccacagag atttaaaact ccgtggcgtt
    21481 gtgctttttt atagctaaca ggaaaatgat tagtaaaaat tttgattttt gattaaggtt
    21541 gcgggcgtaa cagttactgc aaagtgattt attctacaat ggcggcgtcc gtcagtctgg
    21601 aatagtgagc agtatattta cgtaaaaaca gcaaagccgc ccggtttcgg acggctaatt
    21661 ggcacaggct gtgttttata ctcccaaaag caacatgccg agtgcggcag aaaagagtat
    21721 cattataatc ggcgacggtg cagttttttt gattctttta tatccgaaat gtgtcagcca
    21781 cagaatggca aagaccaaaa tggatttagg atccggagca agcggaccgg gtgtcgattc
    21841 tgaaacggca atgaaattta agaattcgct ttccgttagc cagccgtttg aaatgactgt
    21901 ttctcttaca agggaaacca ttccgtatcc gccgccgaag gtaaaggcgc cgattttaag
    21961 aaaggtgaga aacaaagtcc tctcttttct cattgccgca ccgcctgtct tttacggatg
    22021 ataaaaagta taacgatacc gagaataatc aggcagccgg acagcacctg tgatacacgg
    22081 atacccggaa ggagataaag gctgtcggag cgcagcccct ccacaaaaag ccgtatcgtt
    22141 ccgtacccga tcatataaaa cggaagaaga tagccttttc gcctgaaatg cgggcagcat
    22201 atcagcatgg cggtgagtaa agctaaattt aatgcggatt cgtagaaaaa ggttgcctgc
    22261 cgccattggc cgatttcttc tatgtaaacg ccgtaaggga aaaagcagag cgaaggattt
    22321 gtaatttggt ttccgtatgc ctcttgattg acaaaattcc cccaacgccc catagcttgc
    22381 ccaagcacaa ggctcggcat cagggtatcg gcaagtgacc aaaaggaaat ccgttttact
    22441 ttacagtaaa tcaatgcgac tgcgacacct ccgaacactc cgccgtaaat ggcaagaccc
    22501 cctcggttaa tttcaaaaac agaaagcagg tcgtttttgt aattatccca ttcgaaaatc
    22561 acatagtagg ctcttgcaca aataatgcat acggggatca gacaaagagc aagatcgtat
    22621 atatgctcct tgttgatgcc ggtttttctg catcggtata tcgccaaagc aaagccaagc
    22681 accataccgc aggtgataat gattccgtac caagcgatat tcagcccttc aattccttgt
    22741 ttcctcgctt ttctcagtct acccgcagca tacggtaacc gacgccgata tgcgtctgaa
    22801 tgtattgcgg actgtccttt tgcggctcca gctttttgcg cagtgtcgcc ataaatactc
    22861 tcagggaagc aatatcgttt tcccagctgc tgccccaaat ctgctgcgtg atataggtgt
    22921 gcgtcagcac ctttccgaca ttatgagaga gcagacacaa aagcttgtat tcaatcgggg
    22981 tcaggtgcaa ttcctcccca tttagataag cgcagccggc ggcataatca attttaagct
    23041 tgccgttttg gaaaacgggt gaatcggcgt cgccgcttgc ctgaagaagc agcagccgtc
    23101 tttgcgtgac ccgaaggcgt gccaaaagtt cctctacgga aaagggcttt gtcagatagt
    23161 cgtccgcccc ggcatccagc gccgtgattt tgtctttatc ctcgctgcgc gcactgatca
    23221 caataatggg catatttgac catgagcgaa tattttcgat taccagcgtg ccgtcgatat
    23281 ccggcagccc cagatccagc agaacgatat ccgggttatg ggaggtcgct tctataatag
    23341 cgctgtgtcc gttctccgcg gtcaggtagc ggtaatcgtg cgcctttaat gttgttacga
    23401 tcagattgcg gacgggacgg tcgtcctcca cgacgagtac aaggggttta ttcattcaga
    23461 gtcacctcgc tgagtggtaa agtaaaggta aatatgcaac cgtgcggtac gttatccgta
    23521 agcgtcagcg ttccgccgtg cagctctacg atagatttac acagggtaag cccgatcccg
    23581 aagcttctgc ggctgtctgc gacggtattt ttcccggtgt aaaacatttc aaaaatatgc
    23641 ggtttcatat cgtctgcaat tccgtttccg ttatcggtga ctcggatcac ggcatccccg
    23701 ccctgttttt cggcacttac gcagatctcg gagccttgtt cggtatattt caaagcatta
    23761 tcaataaggt tgacaatgac ctgcacgata agctgtgcgt ccatacgggc aagaatcaaa
    23821 tcgtcgcacc ttaccgtcac cttgtgtccg acggattttt tcttcaaatg cgaaacggct
    23881 tcgttgacca cttcgtctac aagctggtcg gtaagctcca gcttcaggcg tccgtcattc
    23941 agccgtgtaa catacagcag attttccacc acgccgatca gccattcgga atcatcatag
    24001 atatctttat agatctgctg cttggttgct tcgtccaggc agcccccgtt atgcaaaagc
    24061 gtgtccgcat tacccgaaac ggagcaaagc ggcgtgcgca gatcgtgtga gatcgaccgc
    24121 aaaaggtcgg cgcgcagctg ctcgcttttt gcaagatctg ccgcccgttc cttttcggct
    24181 gcgttgtgag cattatccat tgccagcgca cattcgttca cgaccgacag cacgatgctg
    24241 ctctcaaacg aatcgggctt ttcgggtctc atcggtatcc cgattacacc gtatactctg
    24301 ccgcctgcac ggatcgcaag ataaagacat tcggatttgc caaactgtgc tgtcgttgct
    24361 cctgcacggc ggccgttttg aagcacccat tctgcggtct gccgctctgc atcgcttagc
    24421 agcttttcag cgtgtgtatc ctttttttcg gcatacagcc gcccggacaa cattccgttt
    24481 tcgcctttcg tataggcaac aatactgcgg tcaagcagcc gcgagatctg catacaggtc
    24541 acgcttaaaa catcatcgtt gctttttgct ttttgcagca gacggtcagt gtcaaacagc
    24601 acctgcacac gaaacgcgga gcgagccgag agccttgcat gatctttcag cttcgaggca
    24661 agcgtgccgg tgagaacgga agacgccagc atgactgcaa aggtaacggg atagcctacg
    24721 gcataggtct gaaaggacaa cctgggttcg gtaaggaaaa atccgaacaa tacaacactt
    24781 agaagtgagc ctgccatact gcaaaggtat cctttggtca aaacggagat catcagcaca
    24841 ccgaggatgt ataccgtaac gatattggta tcgggaaagc cgagatgatc gaacagaaac
    24901 ccgatcgccg tgcatacggc aagcgccagg acggttatgg caatatcccg cagcgacggt
    24961 atctctgcac caaggaacaa acggcgtctg tgcggctttg tatagttcag cgcatcggga
    25021 ataatatgaa tgtcaacatc aggggcggag gcgatcagct tttcggtgag agtcggtctt
    25081 ccgaacagcc ctctgcgtct ggcactgctt tgcccgatca cgatttttgt gacatcggaa
    25141 agtcggacat attccgagat ctgcagcggt acatcctcgc cgtgcgtcat aacgatctcg
    25201 gcgccgagcc tttgtgcgag ctgtatgttc tgcgaaagtc gggactgatc ctcatcattt
    25261 atgaccgcat ccgtctgcac atagatggcg gtaaatcttg catgaaggac cttagccatc
    25321 tttgcggcgg tgtgtatgat ccttgcattg gacggcgaag aagataggca gacaaggata
    25381 tgctcgtctg cgcctttttt tgactgctcg gtttgtgttc tcatccggtt caccgtcctt
    25441 ttgctttatt atacatcaaa atcgtgaaat aatataccgc aaactaatac tcatcttctt
    25501 tcccgtcgga aagaataata tcgacatttc tgtgcgtttc acgaaaacgg cggatcgcac
    25561 ataatatttc actttcggac agtccctgcg gcaacagaat ttcggaaggc tcacgggatt
    25621 cgttttcctt taacagtgct tttctgtttt cttcaagaaa tcgatccagc ctgcacatga
    25681 tataaaatcc gaaagcaaat atgccaagca gactgacgat cagcaatagc tcagccatcc
    25741 tcattcacct ctgttaaatg cgaaaacact tttgcagttc tttgtaagca ccgagaacca
    25801 aaagagtgat attgtccgta agaacggttt ccggtgaaac tgccatattg atttttccgt
    25861 tctccttggt tgccataata ttgatgctgt attttctgcg gatatccagt tctccgacgg
    25921 ttttgccgat ccattcctcc ggcacctcta cttcaaaaat ggcatgagca tcatcaacct
    25981 caatgtaatc ccgaatatga tcggcggtgt accgaatagc agcccaattt gctacctgtt
    26041 tttccgggta aacgacctcg tccgctccgt tgcgcagaag aaatttttcc tgcacatcac
    26101 gctcggcgcg ggacacaact aattttgcgc cgagttcttt cagcaaagaa gtggtttcca
    26161 gcgagttttg aaaatttccg ccgatggtga cgatgcacac atcaaagttt ccgatgccga
    26221 gcgactttaa aaactccgtg tttgtgctgt cgccgatctg cgcgttggtg acgatcggca
    26281 gaatctcatt gactctttct tcgttgctgt ctaccgccat tacctcgtgt ccgagcttat
    26341 tcagctgcaa agcaatatgc ctaccgaatc tgccgagacc tattaacaat atgtttttca
    26401 taatatagta actcctttat ccgactgtga ttttttcgag cggcagcttt gctccgctct
    26461 gatttttgtt ggaaatcgcc gcgtaaatca aggtaagtcc gccgactctg ccgagataca
    26521 tcaagacgat caaaataagc tgagaaagaa caccgagctg cggtgtgatt ccaagggtaa
    26581 gcccgaccgt tccgacagcg gacgccgttt cgtaaaggca ggtgctaagc ggcagacctt
    26641 ctgccgtgct gataacaatg ccgccgacaa aaaacagcag gaaatacatt acggcaacgg
    26701 ttgcggcgtt tttcactgcc gaaccgtcaa tgcgcctgcc gcagacttcg gcatcgtcct
    26761 ttttgcgaca gacggagaat gcactcagaa tcagaacggc aagcgtagtc gttttcatac
    26821 ctcctgccgt ggagccgggc gatccgccga cgagcatcag cagaatcata attgccttag
    26881 aggaaccggt catagcggga agatcggcgg tattgaaacc ggcggttctc ggtgtgacag
    26941 actgaaaaag cgacgcaaga atcctttcgg cgagcggcag ccccgtaaaa tcgcagaaaa
    27001 aaaagaatac tgcgggagcg ataatgagga ttgccgttgt taccaatatg accttacttt
    27061 gcatacggta ttttttgaaa tgccacttgt ttgtgtaaat atcgtcccag gtcaaaaatc
    27121 caataccgcc cacaataatt aaaagcataa ttgcaatgtt tattgtaggc tctccgatgt
    27181 atcccgtaag agacgggtat ttgttttctg cggtgccaag tatgtcaaag ccggcgttgc
    27241 aaaaggcgga aatcgaatga aatgccgcca tccaaattcc cttaataccg aagtttccgc
    27301 aaaatgtcgg cagcatgacg atcattccga taagctcgat caaaaaagtt ccccgtacaa
    27361 taaagcgggt caggcggacg atcccgccga ccttcggggc agagatggca tcctgcatgg
    27421 tactgcgctg catcagcgat atttttctgc cggacagcat agcgaaaaat gccgcaactg
    27481 tcacaacacc aagcccgcct atctggatca tcagcagtat taccgtctgc ccgaaagcgg
    27541 accaataact gcccgtatcc tgcacaacca gccccgtaac gcaaacggcc gaggtcgatg
    27601 tgaacagcgc ctcgtgaaac ggcgtaacaa ctccggcagt cgatgaaaac ggcagcatca
    27661 atatcagtgc tccgagcaga ataaccccgg caaagcccaa aacgatcagt ttaaaggatg
    27721 acagtcgctt ttttctatgt gtaatttcag gcatatatac ttctcctgtt tttgtttgtt
    27781 tacaaagagt atattatgaa atataaaaaa acggtgtaat gatccggtgt gctgtattaa
    27841 gattgcatta aggcgctcat tctttgaaaa aacagtgcag agtatagcaa atgatatcac
    27901 ttaggcatca cccacttctg gtccaatgga ccagaagcta ataaaccatc tccaataatc
    27961 tctcatatgt tgactgcagg gatccccggc tgtgtaagaa ggcataaaga aaggccccga
    28021 ctctcaaagc aatttgttct aagaaatcgg agccttccag caggaacagc ctagcacagc
    28081 cgtaacctac agtaaacata tgtaatttta atcttcatca tcaaaagagc ttccaaatga
    28141 gctattgctt tcaacaatat gcatctcgcc agattccata tccattgcta ctccacctcc
    28201 tatatcctgc ataagattcc catctgaatc catcatcata ttgccaccca tatcaaagca
    28261 gtaatctcca tctgagtaat caaatcttaa ttccataagt tcacctccta tttaatctga
    28321 aaaatatcgt gaccatcctc tgtttttttc acttggtata aacaatcgtc aatccaaaaa
    28381 gtaatcttat ctgcagcctc atcgtttgtg gcaatattag tacagatcaa attactaatt
    28441 gcattagttc ctccgcatgt ctttggaagt atatgatgaa tattccatcc gcaatatgtt
    28501 ctctccttcc catcacaaac ataataatcc ggatctccat acccttctcg gcacattaaa
    28561 tatccatgga aatcctctgc gaaattgttg tcaccaaaac acatctccca taatctcaga
    28621 gcatttttct tattgattcg cattcttttc acctccaagc ctgacccaaa ataaaaaagc
    28681 ctgtacacta tgcatgtgca gactaacagg tcagacttca gactttacac acatagttaa
    28741 taaagtgctt tgtaatcaaa acacttcaat aaaaatatgt gtaaagcttc gaaacaataa
    28801 tttacgcacc taaggtttat cattactttc tccagaaatc aactagaata aacgtccccg
    28861 gatagtttcg taagatttac tttatacaca ataccattac tccttttctt ccgatagcat
    28921 atcagactaa gtctatgcat gacctctagt aagaacacca taaacagctt cataacatgt
    28981 accatcggag accagctatg aaaagtcaac cataaattag caaaaaaatt ctttttcata
    29041 t
//
LOCUS       DAWEJW010000026        27478 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_723_length_27478_cov_57.434052, whole genome shotgun sequence.
ACCESSION   DAWEJW010000026 DAWEJW010000000
VERSION     DAWEJW010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 27478)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27478)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27478
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_723_length_27478_cov_57.434052"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         8165 a         4986 c         7694 g         6633 t
ORIGIN      
        1 gagaagattg atgagatgat caagaggaag agggaattgt ttacgtaaaa caggggaaat
       61 cgtttgtata gtaataatat caaaaaaata agaatagttg ctgatgttgc cagtgttttt
      121 gcggaaagtc ttgaaaggag gaaactatat tgggctgaac agttgcatac tggcatcgta
      181 ttatagcatg cgaaggagaa aggggaattc tcaatgttaa gagttgctat atgtgaggat
      241 gttttatctg agttgcagaa gcaaacacag atcatacaat ccatcatggc aaggctatcc
      301 aaaaatgtga aatttttttg cttccggagt ggggaagaat tattaatgga aacagacacc
      361 acgggaaata tggacatcgt ttttttagat gtggaaatgc ttggtatcag cggaattgaa
      421 accgcgcgta tccttaggga aagagacagc agggtaattc tgatttttgt atcctgtcat
      481 aaccagtact gcaaggaaat gattgaagtg cagccatatg catttataga taagccggtg
      541 tctgaagaaa aattggagaa gatcctgaaa cgggtgtttg aaacacattt gaattccgat
      601 gacagttatt gcttttctta taataaaagg caatacaata ttccactgaa tcagatccgg
      661 tttttccaaa gtgataaaag aatcgtacag atcaatgcag tacagctgga tatgcctgaa
      721 ttggaatact tattttatgg aaaattggaa gaagtggaaa ggaatctgcg gaaagcaaat
      781 gtaaaattcc tgagaatcag aaaatcgttg ctcgtgaata ctcgttttat taaggagtat
      841 tctgcggaca gggttgttct ggataatgga cgggaagtag aaatcagtaa aaattacaga
      901 gatcatgtaa gacagcatta tatttcaatg ttaaagggga gaagatggga atgatgcttg
      961 ttctggctat tgtggagatt ttttctgtaa tactgcagcg tattttttgt gacagtacat
     1021 tgaaaaagaa gaaaattcgc aggtatacag actatctggt atggggagga tattttgcgg
     1081 tcttcaacgg tgttacatat gttttaacgt atctgggaga tggcacgagt aatatctggc
     1141 tgaatatatt actttttgta tgtatttttt ttgtgacgat cagaatccta tatactgatt
     1201 cagtcagaac attgatggcg acgactatat ttatgtatat gagcggcatg tgtgcagaat
     1261 tgctcgtgta ttacggaaaa gaatttctgg catggacaga tgatgcagaa gtaaccttgc
     1321 tgtgcacagt gttatctaaa attgtctggt atttgattat taaatttacc tctctcatta
     1381 taaaactgaa cagaaaagcg gaactgaatc ttcaggattg gttggaagta tttatcgttc
     1441 cggtctgtag tatatggatc ctgatttccg tttttatcac aggaaccctt gaaaactatt
     1501 tctggggatt tattgtagta ttcatgatcc tgctgatcaa cgtctttacc tactatctgt
     1561 atgataaagc aaaagaaaat atggaaaaac aaatgcggga agaaatcctg aaaaatcagt
     1621 gtgagtacta tattcgccag aaccgggaaa gtaaggaatg gtgggaagaa ttaaggaagc
     1681 tgcgtcacaa tatgaagcag cattacatat tggaaaatac ttatttggaa aaggaagatt
     1741 atgaagccct gaaaagatac tgcagtgaaa atctgaagtt tttagacaaa aaagatttta
     1801 tttccaatag cggaaatctt tatattgaca gtgtggttaa ttacaaggca gatatggcgg
     1861 aaaaggcagg aataaaggtg acagccaata cggaagtgcc gcaggatgcg gagatgaatg
     1921 cagaggatat cagcatttgt ctgggcaatc tattggataa tgccatagaa gctgtaaagg
     1981 aactgaccga agatagggtg atcaggctat ggatcaccac agacggtaat aatatagcca
     2041 tcagtattaa aaaccggtat aaaaatgcat tgcataaaag cgggggacga tatctgacaa
     2101 gcaaggaaga cgacaggatg catggaatcg gtctttcgat cgtcagacag atcgtggatc
     2161 aatatgccgg tgagatggag atacgggagg aagataatgt ttttgatgta atgatattaa
     2221 tgtatgattt tttaacataa aaagaatacg gaatatgtga atacccggat taagatatgc
     2281 ttttcaagag aaacgtttcg aagcatattt taacccgggt atttttatcc aaaagttaca
     2341 tataacccgg tcgtttttga catctacggt tatgacttgt cgatatgtgt tataatctgc
     2401 aaaaaataag gaggaaacac atatgaagaa attcaaagaa tttttcaaga agtacgcatg
     2461 taatgcatca gcaattttgt tgacgggagt gttggccgga gtagcaacaa gaggatgcta
     2521 ttttgtgtac tatcagcctg aggtacccga aaatctgaaa aacttttcaa aaaataagta
     2581 agcaatggaa cgattttttg aaaagataac cagggcattg gttgagaacg gtaatatccg
     2641 tgaggaagaa gaggaagtat atcagtacgc cctgaaaagt atgctgatat taggaggaaa
     2701 tattcttctg agtctgatga taggagtttg tatgggaatg ccgggatact gtgtattgtt
     2761 tctgtgtatg cttgttcccc taagatcaga tgcaggcgga tatcatgctc ctaacttgtt
     2821 tatctgttat ttactttctt ttgcatcctt gattttgaca atgttttggg caaaagagac
     2881 aagtgtattt caacttgcca tcgtatcagc acttgcattg ggctcattat tattaatttt
     2941 tctgtttgca cctttggaca gtaaaaacag accgctggaa ggtcaggaga gaaagtgtat
     3001 aaggaaacga gccagaatag ttgtgtgtct ggagtggatc acagggggac tgctactgct
     3061 tgtcagccct cctgccgcct atacagtatg gagcgcaatt gtgtggagtg gagtgggata
     3121 tatagcatgg tatctggaaa gaaaaataga ggcaagagat gaaaagcatt aaaggatata
     3181 aaaagagatg tttacctgtc tgtttactgc ttatgctgtt cataaatgtt ttgcctgcta
     3241 aggcagcctt ggatgcagcg aaaagggcag attacactgt atcagaattt ggtctttttc
     3301 agcctaagaa gcctaaaaaa cagaagtaat aagtaaggta agagaagggg aggacggaga
     3361 tgaaaagaat agggatattg ctctgctgta ttgtcctgtg tctgctcttt ccagaaaagg
     3421 tgcatgcaga agagatagtc tccgaaaagg aacctgttga tattattttt gttattgatt
     3481 gcagcgggtc catgaaaacc aatgatgtat cacgaatggg cctgagtatg gtacaggcat
     3541 ttgtggatac agtacaggct gaagatatcc gtattggata tgtggcttac aatgacagca
     3601 ttttatctta ttctgcaccg aaatccatag cattggctga agaaagagaa gccttgaagg
     3661 aagaaatagg agcaataact tattcccggg atacggatat tggattagga gtctcctacg
     3721 cctgtgagct gctgtctgcg gagaaaaaca ccagaaaaat catggtgttg atctcggatg
     3781 gagaaacaga tctgccgcag ggtaaggaac gtacggagga acagtccaat caggaactgg
     3841 aacagtgcgt ctgtcagtgt caggaagaag gcatacagat ctatacagtg gcttttggtc
     3901 aatatgacgg tagcaaaacg gtcttagaag aaatagcaat gcagacagaa gcagaaagct
     3961 attctgcaca agggccggaa gacctgatag aaatattgta tggaattttt caggacaatc
     4021 ttatatacca aatacaaaaa ttttccagtg gaacttatgc gggaggaagt caggaaatca
     4081 ggtgtgtgct ggatgcactc tatctggatg aaattaacat agtattgatt tcctcaaaac
     4141 ctataggaga agcaaccgtt caatatggcg gggaggaaat cctgctgaca gggctgtccc
     4201 attatgccgt aggaaagatt gaaaatgtcg gagagaatca gacaggaaaa gaactgatta
     4261 ttcactcgaa aacagaggaa ggacaggatc ttcaggtata tgttatcagc taccgggggc
     4321 ttacaccggt attggagatg acaacggatg ccgaaagaaa tcagcatctg gaatattggg
     4381 tttactttaa agaccgcaat gaaaacatta taaagaacac agagttttat aacagctttc
     4441 tttggaaact tgccgacgat gatgcggaca tggtacagga atatgtgaat gtgtcagagg
     4501 gtgtcctgaa ggggagtctg caatttcctc attccggtat ttatatgttg agaggaacac
     4561 tttcggatga ttttggaaac tattcatttt cagctcaggt caaagtgata aatgagattc
     4621 caaacgggag cataccggaa gaagattgta cggttttaga cggtgaaaga attcttaatc
     4681 tggatgaatt tttcacagat cctaacgggg atatattgac ctattctgtt acaggtgtgc
     4741 aggaaggtgt tgaagttggc ctggagggga atctcctgac gattactcca aggagtgcag
     4801 ggacacatat tgtcacattg caggtcagtg acggtgaaga tgcgatacag tatgcctatc
     4861 ggataaaggt aattccgata tggcaggcat attggtgggt ggttgcactt attcttatag
     4921 tcggcatatt tgttttgtgg aagattttac ataaacccag gcctgaattg gagcgcctga
     4981 cggaagagaa aaagcaatac catttctgtg ggaaattgga tgcctatttt gtattgcagc
     5041 cggaagatga ggaggagatt ccaccacttt ctttttccat gaataaggtg aaggacggca
     5101 gggtatcatt gggagcgctt tttggtactt atccggaaca ggcaaaagca ctccagttgg
     5161 aagatatttt tctgattgct gatgaaaacc gcaacataat cttataccac agatcaaaat
     5221 ccggtgtcat ggtgggaaat gcaattgcat gtatgcagat acagtacagt attagttttg
     5281 gagatatcat ctacattact tcatccgatg ggagatatga tctggagatc cactatgttg
     5341 cagtatttga atgatattta taatattacg gcatttattg taccgagaag caattgtaga
     5401 catcagttgt ggacaaaaat tgctcacaac ttttcaataa atcaaagtaa aaggaggatg
     5461 tgatagccat ggagacaatc acacagacaa acagaacaat actgtttgcc gaaatcaatc
     5521 cggaacgact caatctgctg actttagtag gggatgtgcg gggaaagagc agtctggatg
     5581 acgacaagat caaagagatc aacgaagagc tgttggtctc aagctttgag gaatttctgg
     5641 aaaagtttac tccggtggta tattcctggt gcgacgcagc cacaggaacc attcagtaca
     5701 gtctgacaaa gccggaaaat attccccaga actgtatcac ggaaataccg ctgaatgaat
     5761 ccaatgatct tttaaatatg ttgacgacac tgatgagcgc aagagatgcg cagggcgtag
     5821 ccaatgcaga tttccagttc agcaatattc tggagatgat ctcccctaag aaaatcatgg
     5881 atgacatccg gcaggtccgc agggaaatcc agtataccta cagtaaatat gtagaactgg
     5941 atgaggaaga tcccaaacgt ctggatctgg gtgataaact gaactatcag tttgaacagg
     6001 caagtcagaa ctacaacaat gttctggcca tgctgccgtt ggcgatagaa gacattaaga
     6061 cccggttgct gcttggagac agtgaagcag ggcagggaca gtctgaattc aaggccggac
     6121 ttctgagtat gggagatgat ggagaactaa agattctgga gatgaagcag gaggagagta
     6181 cccagcttgc ggtggtggat gatcaggtta acacaagtct tatggagacc ttccgggagg
     6241 attatgatgc attaaatgat aatccgtccg actatgtgag ggatctggtg gtgcggacat
     6301 tctgcccgtt aaactccaca atggagagca gcatcgacag ggagttggaa gtacgcaatt
     6361 ataacagcta tctggagttt tacaaaaaaa gtaaggatga ttttataaaa gttgtcaagc
     6421 ccctgatgga aaaaatattg ggagtgaaga ccttttttga ccagtatcag gtcaaggaaa
     6481 gggggatgca gccgaagctt ctgattacga acctgcccct tgagatgatg gtaaaggcca
     6541 gcaatctgcc ccgcctgatc acatacctga ataccactaa cgacaagaat gaatttgaca
     6601 atacaatctg gtttggaatc gtacctgatg tggagttgaa tcagacagaa ggaggcagac
     6661 ttcagagagt tcgttttgca ggcaacaagc gggaggagaa gagcggtaat aataccatgg
     6721 agaacgtggc aacgctgatg aatgcgttgc agccctaccg cattacaacc ttcttcagct
     6781 tttgttccgg cgaagagtcc accttcagtt atattgctac agaaggtgtc aacatctata
     6841 aggaaaagtg tgcgcctcta atgaagaagg cctacagtga gtttgccgtg ccgtgcattc
     6901 ccaacgcaac catcattccc aaggacaagt ccggattggt tctcgataag cggatggtca
     6961 tggatgagga tggaaatgtt tctctctctg cggagaagga tgatgtgatg aagatgtggc
     7021 tggaaggagt ctatgtgggt gcggcctatg aagcggcagg cattgtggca gcatggcagt
     7081 gtccggagta tctgaagcag cgtttcagaa atacaagcat ggaatatccc ggtgtgcggt
     7141 ttgatgtgga agccaatgac aatgcgcttc tggcgaccac cagcatgacc aaagagatct
     7201 ccggctttac caataccatc aagaatgcca tcaaccgcac cggcttcggc tttgtattct
     7261 cttctgacaa tgcgcaatac aagggccgga atgtcaagaa cataacggtt tacaaggcac
     7321 gcaacctgtt aagtaacggc aatgagtttg aacccatcta taagacgctg gtcgttacat
     7381 atattgagcg tatgctgcga ttctacagtg gcgattttaa gcaggataag attctgacat
     7441 ttttcagcaa taatcccaac agccagaaga gcagatggga tgcagaccgt cagtatgtta
     7501 attctattct tcaggacggg gatgaactgg atttcagtat tgacgagaaa aacggtgtct
     7561 gcgatgtatt agtgaccttt gcgaatacca ctaaaaatct gaaagtacag cttacgagaa
     7621 aagctggaaa tgaataatct tgtacatgcc gtacagataa aaaataggag gaaacaaaaa
     7681 tgggagtacg attgagtatc gaaggaagcg agtctatcaa gttacaagag accagcatca
     7741 ccggcattga gtttggagcg gatatccctc atgattccaa tgcaagatct acggatctgg
     7801 ggtcaacggt attgatcacg gggaagattc tggcagccgt agacggagaa acagcggatg
     7861 acaccagcaa gcttgcaaag tggtctttgg ttcctgctga gaaatccgac tgttaccgta
     7921 aggtgcagat caatgtggtg agtgcatctc agattgtgcg tcagatcacc attcccaatg
     7981 cgttcgtggt gggatacgaa gaggatttta cggatgagaa cggagcaggc actttccgcc
     8041 ttctgatcaa gcagaagaag gataagatga gcaatcttaa atttgagggc ggcttcggtg
     8101 aggagtaatc ggcaggccgg taaaataata catagatagc gcatagcaga tgaaaggaga
     8161 ccaattatgg gatttacatt aaaggtggag ggaaacacca cgattgaact gggaaccaca
     8221 agcattaccg gagtaaaggt cagcactgac attccgcagg acagcaacgc cagatccaca
     8281 gatctgggca gcacgatcac tgttacggga aagattctga ccgccgtgga cggagatccg
     8341 tttgacgcaa ccagacagct gggcttatgg gcactggttc ctgcagaaaa ctctgactgc
     8401 taccgtaagg tcactgtgga agtgattgct gcttcacagg tggtccgtaa gtattcctat
     8461 cccaacgctt ttgttgtgga ttacaaggaa gattacggcg atgtggaagg catcggcact
     8521 tttaagctga tcatgaagca gaagaaggat aagatggcgc aggtatccgt agaaggtggg
     8581 tacagcatta attaaacagg aaaattccgc atgcagcttt ggcggaaaga cataaaaggg
     8641 ggctgcctgc ttgcgggtgg tccccattga taaatctact gacaggcggc agtacatgaa
     8701 agattactat aaaatattgg gcgtagacag acaggcatca acggaacaaa taaagaaggc
     8761 atatcgcagt ctggctaaga aatatcatcc ggatgtggta aaggatgata aggcaaagca
     8821 agagcgcatg tatgaaattc aggaagccta tgcatgtctg gaaaatgaag aacgccgtaa
     8881 gaaatatgat gcagagtgtc tgaaaagccc ccgaaacact gtgaacgcag gggagaaagt
     8941 gaaaaacggc agaacctctt ttggagagaa gagtccggat atgggacagt tcgaacggtt
     9001 tttcggattt caacccggca gggggatgga aacttatcag gaccatagaa tctcaaagcc
     9061 ggaggggcct atcaaacccg aggaaatgtt tgcagccttt ttcggaagag gaagcatgca
     9121 ggaggcagaa aaaagaaatg cggcgggaag caaaaggaaa aagagttaaa cagacgctgg
     9181 atgttctgat tattttatgc ggagcaggtg ctgttttcct tgtctttaag gaaataggga
     9241 catggcgctt tgcacctgcg gtttattttg tcattgttat cggaatggca gcggcgatat
     9301 atgatctttt ccggctcagc ggcagcaaga atacggaaga gatcatgcca gaggcggaaa
     9361 gaatacaggg gatacaaagg ctgattctgc tggatgagga aggaaagccc attaaatcat
     9421 gggacttgca gggaaagatt tccctgatca tcggaaaagc cgggcgggat caggagctgg
     9481 atattgatct gtcagactgt gagtacagca gctttattga ctttcagcat gctgtgctga
     9541 atttctgtct ggatcaatgg tatgtggagg atcttgggtc tcagaacggt gtaaaagtag
     9601 gaaaggtgga agacggggaa tgctacaggg tgatccaccg cccctgtaag gttgtggcag
     9661 gagatgttct ttacattgcg aatacgaagc tgtttctgac gtaatgtgaa gtagagataa
     9721 aaaggatagg agatgggaga aaatgggttt ggcgaaatgt ccgaatggac acatttataa
     9781 ccccagacgt tatgggaaga tctgcccgta ctgcaatatg aaaacgcagg acgaggcatt
     9841 tgcggaaaaa ccgctgggat ttgagcctcc cgtggaaata ctggaggaga cggtgcgtcc
     9901 tgtgtgcggc tggatcatct gcattgaggg tgcaaaggta ggtatggatt acaagattca
     9961 tgaaggtaag aattttgtag gccgggggga tgaaatggat atccagattc tgggggataa
    10021 tgagatcaac cgcagaaatc acgctatcat tgtatatgat cctaagaacc taaataccat
    10081 gatacttccg ggagattctt ccggtattgc gtacttgaac gataagcctg tatatgtacc
    10141 gctagaactg aatccctacg atatcatcaa tctggggcag agccgttttc tgtttgttcc
    10201 tctgtgcgga aataatttct cgtgggggga tctgaaatga cgatgggtat gcaggcgttg
    10261 tcaggtctgg ggcttgtata catcattttg atcttctgcc gctgcatttt gaaatgggga
    10321 gatggatcct gcggaactga taagcggccg gggatcggta ttgcgcagac taccggcagc
    10381 cggcaggtac aggcggacgt tgcccagata tgggacagca gtgccgggct gatggcggtc
    10441 atagcggatg gaatcggcag tgccaatacg ggtaaagtgt gtgcacaggt ggcagcggat
    10501 gcgattctgg atcgttatga gccatattgc gagctaaacg atccgatata ttttttcagg
    10561 acatcgtttc tggaggcaag ccagcgtatc cagagaacga taggagagcg cagaggcggt
    10621 gccagcaccg gcgcagtgtt tataaatcgc agccacctgt attatgcggt ggcaggagat
    10681 atccgtgtcg cagtcatgcg gggcggggag atgattccgc tgagcaaggg gcagacgctg
    10741 gatgttctgg cggcgcaggc ctatgaagac ggtaaaatat ccagacagga tacgctttgg
    10801 agcatggagg agaaaaaggt ctggagttat ctgggacagg atggcttcca tgagatagag
    10861 gtatgtgaaa agcctgtgct gctaaagccg gaagataaag tgttattgat gagcaaaggg
    10921 atctttgagg aactttcctg gagagaaata gaggatatcc tgatcgacac ggatgattcc
    10981 cagaaatccg cagactgcct gatccgtgca gcagagcaga agggaaatcc ccagagggac
    11041 aatggaagtg tcatactgat acaaaggttg gaggcttaaa tgagacgaat caattctgat
    11101 tttcagacgc ttcatatatc agaagaggga cagaagctct ctaaccgaga ctatttcggt
    11161 tatgtggaga tggatgattt tgcctgctat gtattggcgg acagtctgga tgacgaaccg
    11221 gccgttaaca gtgcaagatt ggtagtagac agtattatcc gtgactttac ggaagcaccc
    11281 acgatgggaa agggcactct ccggcgatat ctgctcagag cacatacaga gctcctgaag
    11341 caaagagcgg gaatgcattt aaaggttgct gtggtggtgg cagtgaccga ctaccggacg
    11401 cttcgctatt gtcatgtggg caacagccgt ctgtacctga tccggaacgc ccggattctg
    11461 gaacagacga aggaccagtc gctgacgcag aatctgctgg aacaggaacg gatcctgctg
    11521 gatgaggccg caaggcatga agagcgcaac aatctttatt cctttctggg ggaaagagga
    11581 aagccggaga tacagatttc ccggaaaaag aagctggaag caggagatct cctgatacag
    11641 cttaccagag gcgtatggga acagtgtgga gagcaggaac tcctgcggat cgtcaatgac
    11701 gccaaagaga caaaggatat tctggatcag gtggaagact gtatcctgat ggagcagaac
    11761 agtagaagta tcgacaatta ctccatggct gtgactgctg taaacaaggt gtatcagtct
    11821 ccgaaaaaac cggtttccgt caaaaaggtt ctgatgatcg tcctgcctgt tctgttggtg
    11881 gtgattaccg tcggcgtgac cctgtttctg cggtaccgca gtattcagaa caaaacgcag
    11941 tccctgttgc agtatatgga aagcggtgag gagtaccttg cctgcagtaa cttccagaaa
    12001 gtggcggaag aatatgaggc agcgaaaaag ctggccgaca gcctgcataa ggagcaggaa
    12061 tatcgggagg ccgattccta tacgaaactg gcagagcagg tgatcttagc ggatgaagca
    12121 ttgagcgcag cggaatatca gaaagcacag gaactgtatc ttgcagccag acagatggcg
    12181 gtggaaaacg gaaatgtggg actttcctat attgagggac agcttaaccg gacggaaggg
    12241 tacatagaag tgtttgatct gattgctcag ggagagagaa aagaggagta tgacaatctg
    12301 acaggcgcca ttgcactcta tcaggaagca aaggaaaagg ctgcagcgct ttacttcatg
    12361 gatggaaaga aggaagcact ggagttacag atggcagcgg aagaaacgct ggaaaaggaa
    12421 cagttagcgg cggagaaacg cctgcaggaa cagatcgaag cagaggcggt cagccgggca
    12481 ctggatcagg atcagaagac aaatgaccag cagaacgcca taaatatgga gaatcaggga
    12541 aatgaactgt tggcccaagg cagttatgaa agtgccatta ccttttatcg tgtggcccag
    12601 gcctcctata agcagctggg tctgacggag ctggccgacg gaattgataa aaagatggaa
    12661 gctgcccagg ccggaattgc agcaaaggaa gcgcaggcgg cagaggcgga gaagcagaaa
    12721 tcagaataag gatgtgtgga aggggaaaca gatgggagaa agcatgagcc ggtatactta
    12781 taccctgaat cccagtcagg gagtgacgga gaaacatacc tacagacaga gtgaactgga
    12841 aaagatgacg acatttcacc tgcgtgaaat atgcagaaaa gagcgtctgg tggtttcttc
    12901 tgccaaaaat gacgacaagg atgggctgat ccgtttgatt atgcggttca ggggacagaa
    12961 ggaataccga catatcagag aattctgtga aggcggaatg gaacgcatac aggagttttt
    13021 gaagcatcag gtcatccgtt ttctggagac tccggaagtg gatatacccg gtaccattac
    13081 gattttccat gatacggaaa tgaatgaact ggacggctac cggatcaaat ctgaggaaaa
    13141 gcttttcgcc ggaaatctcc tgctggtgga tgaggcattt aaaatatata cctgtttcta
    13201 catagaggaa atagaggatg tggcgtatct gtttaaggga aaagggatgc cggtatgtcc
    13261 gttggagaag catcagtatt ccattctgta tttcccgaat gaagcaatct cggaatttct
    13321 gtatgactgt tattacggaa atcatgtctt cacgcccgga catacggagg ctgttcgcat
    13381 tccgcttctg gatgttcagg aaagacagat tccccaggcc gatctgccgc tggtgatcga
    13441 ctttggaagc tccaatacca ccatgggaat ctgtctgtcg gatggaagta tgcggatcgc
    13501 gacggcgaag ggaaaaacga ttattcccag tgtgatcggt gtgcaggaaa aagcgggcgg
    13561 agaaacggaa tttttgtttg gctatgatgc acaggagatg aacaggcaga actaccggga
    13621 tgaggatgca gctgtatttt atgatatcaa gcgttggatc agcgatgcag accgggtgga
    13681 gagcgtcata ttaaagagtg gatataaata ccagtttccg agaaaagaga tgctccgtgc
    13741 ctatctggat catcttctgg aaatggcgcg tcagcagttt aagtgcagct ttaccaatat
    13801 tcagctattg gcaccgatcc ggcagaaaga gaagttccgg cgggtgttca aggaactgct
    13861 tccggaatat accgtgaact gcgagctgga cgagggaatg gccgtattgt tccacagcat
    13921 tcacagcatg atccgggcaa aggcatatga ggaacgcaga tggtatcacg cccttgtgat
    13981 tgactgtggc ggcggaacca cagatttaac ttccgggcgg ttccggattg agaataacag
    14041 ggtgtcctat atcattgatc tggaaacacg ctatgaaaac ggcgacacaa atctgggcgg
    14101 aaataacctg acctaccgta ttcttcagct tttaaagctg cgtctgacgg aagaactggg
    14161 atttcagacg aaagaagcct tattcatagg gggcgcagag gaaggcaaat ccgcttacga
    14221 ggaactggag agacgatacc ttcaggcaga aaagtggctg cctacccggt ttaaggagta
    14281 tgagggcagg agcagggagc agtatttcta cgtcaagaat aattactact acctttttga
    14341 actggcagaa caggtgaaaa agctgttttt tcaagccgga ttctgttaca ggctgaaaat
    14401 atccacgaat aagggagagg atctcttctt agataaatgg aagctttcct tctgtggaaa
    14461 aggagatggc ggatccgctg cagagcagct ggaaacgatt tccggacctt tggaaatctg
    14521 tctgtattta catgaaatag aggaactatt gagaccggaa atatatggac tgatggagcg
    14581 ttttctcgat cagaaatttg aaaaaggaca gcttgcggaa tatgagatga tcaagctgac
    14641 ggggcagtcc tgcaaatcga gactgtttct ggaagcgctc aaacagtatg ttccgggaaa
    14701 gcggatacag ggtgtccggc gggacgatgc agggacggaa ctgaaaatgt gctgtctgga
    14761 gggagcactt gcttacttta tgaactgcaa gctgggatac atgaaggtaa acgagaacta
    14821 tagagtaggc agcctgccct atgagattat ggcgtatacc catgaaaagg aagagaaaac
    14881 cctgattaag agtcaggata cgcagaatgt gatcggatac atttcccgtt tccacatcgg
    14941 aaatcaattg gatctgtatc ttaatgatga acagggggaa tggctcagga cataccattt
    15001 tgcctatgac acttctaaat ttgagaagac tacccagaag gagatcgacg aggattatgc
    15061 gggtacggtg attcaggaag aaacagatat cattttagag ggagagatga agttctttgt
    15121 atgggtttcc aaggaacgtt ggggatttgt ggtgctgccc atcctgagag atcaagagga
    15181 actatataaa ggtgcagaaa ccttcttcga ttttgaagac gatacatggg aactgaattt
    15241 ctttgacgga aggaagtagg tgtgcattat ggggtggtca ggtcaggaag acggcatgga
    15301 cgtaatgaaa gagataatcc agaaggagat acaatccata agcagtctgg aagaaagggt
    15361 gatcttcaag aatctgatgg aaggagtctt tttatccctg tatgacacga atcgggagat
    15421 gtacgaggga ttggagcgcc gcatcaaaga agaattggac tatgacaaaa accgctatta
    15481 tgtaaagaca ggcattgtgg agagggaatt tttcgatgcc tcccaccatc tgctgtcacc
    15541 aatggatgaa agtgatctgg cagaaaaact gtatgatatg aaggaaattc tgcgggcggt
    15601 ggaagaagag ggagcgttcc ggctgatgaa agtcatgtta cgctgcgatt ttctggaaca
    15661 gcagaaactg ctggcattgc agcctgtgtt tgaaggcgtc atcgagaccg aagaaccgga
    15721 gaaggaatgg aaggtggaga tccgtctgcg ggaaaatcgg gagtatctga aaaaaataga
    15781 atacctgtat ctgttgttta ccagaaacgg cataccctgg cagaccgcca atgcacccta
    15841 tctgtataag atggcggaca tgatcgtgac cgggctcccg gaaggaatta ccggcaaaga
    15901 gaaaatcagg cgggtaacca tccagtttgg agaatacagc agtatgattc aagaagatgt
    15961 cattccggta tggaatattc agagacttga gctggacagc atcggtttcc ccataccctg
    16021 cggagaccat cagaattatg aacacagcgt ttccctgaag aagcatggaa taaatcatgc
    16081 atatctggtg gaggatgaca ggaatgtaca gagtgtcagt caggaagggg aaagattacg
    16141 aatcgtatgt aaaaccggtg aagcaaaaaa atgggggatc tatcagatcc gttcttccca
    16201 ggagcagaag atagaccgct atacctttcc catcatggga aacggccggg aagaaagctt
    16261 tgcagagaaa taccagagaa agtggaacca gcagatcagg accaaaacag agctggcgca
    16321 tttcataaag ggattcgggc tggaggatta tgtacgttat caggactgtc aggtagagga
    16381 cgactttccg ggcaataggg agacatacgc ggtgaactgc tttattccgg atgagatccg
    16441 gctcagatca acgcagaaga aactggttct ctatttccaa ccgggaaaac aggagaggtg
    16501 gctgcagaga gatattctga gttttctggt atctgaggta cagcggatct atccggaata
    16561 tgactgtggg ggaatattgg tatgaggtac ctctgggaag tactattgga agcgaaaaag
    16621 gaacagattc cggaagagag acttcggttt gtccatgcgc cacagggcag cgggtatatg
    16681 gaactgtccc tgccctgtct caaccagacc tggctgggag aagaggagca gccggaagac
    16741 atcaacatag aggtgaatac ctattatcgc ttttatgaca tattctgtga aatgtttcca
    16801 ccggatgagg cagagttccc ctcactgcgg gaaagtctga caaatttatg cctgcatatg
    16861 ctggcacaga atgatatcag gatgggcatg acaagggaag attaccacaa gagacttctt
    16921 gcaaaagaga ttctggacgg aaacttcgga gaaatagcag gaaacgtatt ccggtcaatg
    16981 agcagcaaag agcaggagat attgctgggc ggatggctga acagcttccg gacaggcagt
    17041 gtcctgccgg tttttctgga tatggttcat ggtctggtgg cggatagcat cgtctatcac
    17101 aacaacgcct atccggatga gattctgatc tataccggat ggaagagaga gcggaacctg
    17161 gagcagagga tccgattcct gatcgatacg tttctggaca tccggtatcg ggtggaaatc
    17221 ttttatgaat atcatttcgg gataatcgga gtagaggaga cgatgcggat cggggagatc
    17281 gcgatctgct gaagggagag aaaatgtttc agaatattta tcctgtgttt gaacccaaaa
    17341 gacttttaaa aaaagagatg ttggaaaatc tcagggattt tcccagaagc ctgtttggat
    17401 tgcagtatca gaattacagc gatggaattc tgtatggatg tgacattacg ggaacggaga
    17461 cagggatgat tcttctgccg ggaatattgt gttacaaagg tgtgccctat tttctggaga
    17521 gaccatatcc tgtttcgtgt aaagcagaag gaaaaatggc atatgtaaag gtacatttct
    17581 gggataagac catcggcagc ggcggtgaag agtatttgtc acaaatcatt gtggatgaac
    17641 aggagccgga tgcagaacag gaactggagc tggcacggtt taaacttcag gcgggagcaa
    17701 gacttcgctc agactatgtg gacttttatg attacgagac cgaatttgat acggtgaatc
    17761 gtatttacgt gccttatgcc gcactggatc atcccggcat ctggccgcag attttaaaga
    17821 gttttgcaag agaattatta aagtatgctg tgacagatcc gtgggattgt gcattctgtc
    17881 tccaatgtct gcaaaggaag gaagccatgc cttatgaggc ggtccgtgcc tatcttcatg
    17941 caaggctggg gcagaataag gaatacacaa atatgcagat gtatggcgca ttgaagagaa
    18001 ttctgggtga agcgggcgga agggaagaga ttcccggaca gacccgaaag aaagagggaa
    18061 cattgctcat gctttaacgg tgtgagggaa aaggaggcgg caggcatgga aaacgaaatg
    18121 gatttgtcca tagcacggtt tatcagagag cagcgggaac gggaagaaca ggaggaaaga
    18181 gaacgcctgc ggaaacagta tgatcccagg gagcggatta cttatacgct ggaggagatg
    18241 atagagggga tacgaagcgg acgacagtat ctctatacat tgttgctgga gtttgaaccg
    18301 aaggacctgt tggaaggacg gttgacggtt ccttttatta aaaatttcta tgatgtggag
    18361 caaaatgaag ccggaatgat tcttttggca agcaatgcac gcaaggttgt ttttaatgta
    18421 tctgatgcac cttgcacaaa agtgaagatg cctctgaata aatggatcga gcagaccaaa
    18481 gaagccatga aggatatgca catatatatt aagcccggga aaaaggcggt ggtaggaaat
    18541 atggaatatt tctgctatac ggcgcctaca tccagaggac gtctttttaa catcgtcttt
    18601 cggttgaaaa aaggcggcag gatatatgcg ggaaacctga attgcccgga ggaagagcaa
    18661 aaaggcatgg gactgttgct ggaggcaatg gtgcatgtga ttgaggaaat gaaccgttag
    18721 aagggaggga gcagcgtatg gatgcgatta cctatgggag tctttttctg atactgggaa
    18781 gtacgcgtct gtcggttttg acatgctgca tcagtgaaaa tcccgggaag cacggattcc
    18841 tttccgcaga tgcagagacg caggaggaac tgaaggatta tcttctgtac gaggaagatg
    18901 gtcatatcgg attatttgta atgctcggag agaccataaa accgttgttt tacggcacgg
    18961 tgacctgtat ggaagtaaag gctcagggag gaaggtgtgt gctgcatctg gaggcactga
    19021 cggaaagcta tcagatggat cttacagtaa ggaaccggtc ttttcaggac gtagccatga
    19081 cctcccacca gttaattcaa aagatattgg agccttattc gcaaagtcag attctgttct
    19141 ccatagaaga taaggcatta gggcagatta tggtgcagta tcaggaaacg gattgggaat
    19201 ttctgaaccg tgttctttcc gcctatggtg ccagtgcgta tattgcaggc aatgaaccgg
    19261 ggatttatct gagagtagga ttgatggata cagaggagga tgcagactgg gatctgcttc
    19321 cctatgttct gcatcgtaat gctgcgccca gagagacaaa aaaaggactg aaagggcaga
    19381 tctgctatca gatagagaca tatgacattc ttccattggg agaaaaagtg cttttcaaag
    19441 ggaaagaact gtatatcgga aaaatcgaaa gatttttccg gcagggactt tttgtcagca
    19501 gatattatct gtattttgca gagggactga ggaaactgaa atattacaat ccgttcctcg
    19561 gtggagtatc cataaacggg gtggttacgg ccgtcagcag gaatcggctt caggcacaat
    19621 tagagacgga tgctttggca aattacagga aaaagtattt tttccccttt tctacagtgg
    19681 cagcatctcc ggatggaagt ggatggtatt gcatgcccag accgggggat caggtaagga
    19741 ttttcttccc caccgcagat gagaaggaaa gctatgcgat tgcaaatatt cagggagatt
    19801 cgtcgcctgc atctgacagt ccgatgagca ggcctgatct gaaggatatt accatgccgg
    19861 atggaaaggc tgtccggttt atagaaggcg gaatacaact ggccgttggc ggaaataaag
    19921 gaactgtcac tctgacaaat gatggaaacg ccgaaattgt taccgatgat gacattgaaa
    19981 tcagtgcggc ggaggcagtg tgtttcagca cggatggaat gatgagtgtg acagcgggat
    20041 cccggataca gtttatcaat gatgcggggg gaaatattac cgtaacagac gaaacagtga
    20101 aaatcgatgc tgctatgatt atcaacaatt agcggaggga tattatgtac agtttgcatg
    20161 atatagaagt aaacgggctg gatctggcag aggtgttgga gtgtgaaata gaatcccggg
    20221 caggagagca tagtacgctg gtgatccttg cccgtgtgac agaagaagag tttgtatttg
    20281 aaatctcaga ttgtcaggat cttgaagtac ttctgcgcga aggagaaggc aggaagatcc
    20341 tgttctccgg catcctgacg gatattcaga taacggaaag cggtcaggtg aagactgtac
    20401 gaatagaagg aaagagccgc agctggctta tggatcggga gaaacattcc cgttctttcc
    20461 agaacgctaa gatgactttt caggagttag cacaggaaat actggcaaat tataaggatg
    20521 ctgatctgat ctatgctgct gagggaaagg cggttggaag tctgatcgtt cagtatgagg
    20581 agacggattg ggaattcctg cagagagtgc tttccaggga aggtattatg atcactccgg
    20641 actgccgaca gcccggctta aaattatatg cgggagttcc ggagcttatg gagtcagcat
    20701 ttccctgtca tattctggat atggaaaagg atatggacgg atattatgag ctgaaggcaa
    20761 acggcagaga ggtgcatgca tcggatttta cccgctatac ggtcgtttca gaacagctta
    20821 tgggaatctt tgatccggtc cggatacagg gaaatccttt tgtggtttat gcatgcagat
    20881 attcctttga agatcaggag atgcagggaa cttataaatt acaaagtgca aaggggctta
    20941 caaggcctgt gatctatccc atgcatttga tcggggtagc gctgaatggc aatgtggtga
    21001 acgtatcagg tacaaaagtg caggtagcaa tggcaattga tggaaacagc aggaaacgtg
    21061 cattgtactg gttcccctat tccaccctgt ctgcatcctc agatggcagc ggatggtatt
    21121 gcatgccgga ggtgggagat gatgtgcgga tatattttcc ttccaaggca gaatctgagg
    21181 caattgcact gagtgctgta agtaattacg atgcaccgca gagtggagaa gatcggatga
    21241 gcgatccgga caaccggtat ctgcgcacca aatccgggca ggagcttgca ctggccccgg
    21301 ggtatataaa gctttcctgt ggaaaagcag catccgcagt gacaattcag acggatggaa
    21361 aggtcaaaat acaatctcag tctatggtga aggcaatggc acaggaagag attgcgattc
    21421 atgcggagga gcaggtgacg cttcatgtat cggaacaatt tattatgcat agtctgagcg
    21481 gcggacagat tgtatccagc aaaggcaacg ttattatgca gggaacagaa gtaatcgtgg
    21541 attaacatgt tttgaaggag agacagatgg acagagaaac ggctttgcag aattacagag
    21601 aagcggtatc gagaaaaata gcagcattcc gcagccatat gggggattct gttctggaac
    21661 atgccgagga ttgggaggct gtggtagaaa aagccatgaa gcttctggga gagcagatgg
    21721 aaaaacaggg aaaggagtat gtatgttttt tgtatttttc tctgttgaag tcagatacga
    21781 tcaacagaaa ctatcgggtt cagcttcacg gtctcgatat gagctggtat atggacaagg
    21841 aaccggttga ggtgtatgtg gatgtaaagg aattgcttac acctttggat gaactttgga
    21901 atgagttagt ttgtgccaat cagggatatg gtgtctctgt caatgaatat gacattcaga
    21961 atctgctgtt tgatgaactg accattatgg acaatatgat ctgtcaggtg cttcgctatc
    22021 gtctgcggga ctgggagaaa aagggaatct ttgagcctgt tacccgttct ccctattggg
    22081 tcctgaggtg gggggaatat cgtgatcaga ccgaaatact ggttcagacg gaccgggtgg
    22141 aaaaggatcc cggcgtctgg aagaccgaac tgtcaaaggc agccagagag ccggaaaaga
    22201 tggtgttcag ttattggtat aaaggcacct atgcagacag aacgatccgg gacatggata
    22261 tgcgctttat tacgtttgag gaaagcaccg tacaaaacat tgtatttcag aactgcaatc
    22321 tggaaggaag ccgtttcccc ggaaccagac tgacgggatg cagctttgaa gggtgtaatc
    22381 tgtggggggc agattttcgg gagtgcacgt ttgaacagac atcttttgcc ggagctgaac
    22441 ttactgccgc tgtgttcccc gcagaaagtg ttccgtttct ggaaatcagt gcagagcagc
    22501 ttcaggtaat ccgtctggac agggaggaaa aatcatgagg tatttcttta ttacgcagga
    22561 caaaaatctg ccgggatcta ttcagtatcg tgattttgat atcaatggga gccgtcatgt
    22621 gttttctaaa gaggacagca gccagttgga cagtgttgtg gcattgtatc tggcagggga
    22681 tggcaacgag gccagatggg attttctaca gcatccggta accatgttct ccagaagatt
    22741 ccggaatatt ctggatgcgt atgaaccggg attgttcttc caggaggtag ttctaatcca
    22801 caaggagagt tcccggcagt accggtatgt acatacgtta atggatcagc tggatgcggt
    22861 gggaagtcag acggaatatt atcccaacgg gacggtaaag cgtctggttt tagacagcgg
    22921 gaaaattgga cagcataacc tgttcctgct gaaagggaac cagagaaagg atccgattgt
    22981 cagtctgcct ctggcagaga gcctgctgcg gcgcaggccg atgggaatac actttgaaga
    23041 agtggaggtg cagtaaatga gtgagataca gccaagtctg ggagaactgg aggatgcaca
    23101 gaatactgcc tatgagaatt tcttcacagc gcgggatgct gttacggcgg ctggggaaag
    23161 agttgctgcc gccgatgaag cggtatgtct ggcagagaaa aagtatatga acggtgaaat
    23221 cagtgtggaa gagtgggagg caacgttaca ggaattaagc gatgcacaag tggctgagac
    23281 agcggcaaat gagaactatg aagctgcggc agcgggggcg caggcggcag cggaagcggt
    23341 ggaggccaaa aaacaggaac ttcgtgacag ccgtgtaaat gacaccgctt atgtggtaca
    23401 ttgtgcccgg atcgagtgcc cttttggtat gcgtgaaagc tatctggcac tggatgctac
    23461 ccatggggta ctgacgcacc agataccgca gatgacggta aaagatatga ttctgaatac
    23521 aaatatcatt aacttcggtg gatgccacag cagggagaac cctgatgttc aggcggagat
    23581 tgaaaaaacc aacgccatca tagaatccaa aaaagactgg cgggacgatg tggtgggcta
    23641 tttcacaaag aaatggaatg agcgtgtaac aatcataaaa gcagggatcg gtcttgcaaa
    23701 aaaactatta gggatgaaaa agaaagaaaa gaccgaagaa gaaaagctgg aagaaatgag
    23761 ttcagatttt gttggtgagt gcaaggccca gttccccgcg gacggagaat ggctggaagg
    23821 gcatgaaaag gtatttatta atggggaacc gttgctgttg cgcagatgca gtatcatgtg
    23881 cagttacgga ggatgtgtaa cgatcttgct ttcgggacag ccggaatagg aggaaaaagg
    23941 tatggagctt gcttatcagg gtcttttcat tcgacttccc tttgaaggtg cggttggagt
    24001 agaagcattt gaaatgaatg cgtcatttaa tgaccatgtc acattaaggc tgttacttct
    24061 ggtggaagaa gaaaagatag aagctctcat tcatggtatt ggggatgggg atgatataga
    24121 ggtatataag gcggaggctg atgggctttt atatgccgga aagattacag acgctaaaat
    24181 ggagcaggat cgcagcctgc accttttgca gctggaagca gcttcttata ccatggaatg
    24241 ggggcttgca ccggtaagcc agagctttct gaacttagac acaacataca ggcaggttat
    24301 ggacaaggtc cttaagaatc agaaggatgc ggaaattctg gactgtgcta ccaaaggtgc
    24361 tgtgattcct gatttcctgc ttcagtatga ggagagcgac tggaatttcc tcgtgcgtct
    24421 tgccagtcat tttcatacct ttatggtacc ggactgccgt gcggcgcatg gacgggccta
    24481 ttttggaatt ccggatctgg gggaagaata tgtcctgagc gatgaagagt ttacagagat
    24541 caaggatatg gaccagtatt atcgtctggg cagagaacag aaaatccttc cgcaggaaac
    24601 ccttaaatgg aaagtgactg ccaggcagga tttctgtctg gcacagaagg tacgcttccg
    24661 gggaatcagt gccattgtca caaggataca gtatcagacg gtagaaggag aacttgtacg
    24721 gacttatgaa ctgagccgga ggaagggggt gctctgtgcg ccggagaaga atcctcatat
    24781 tttcggtatg agcatccccg ctacggtaaa agagcgttcg ggaaattgtg tcagggtgca
    24841 tttccatatt gatccggaat atgataattc ccctaatgta aaatacttta cttatgcgat
    24901 cgagagcagc tttatctact gtatgccgga ggtgggaagt caggtacata tttacttccc
    24961 cggggatgat gagaaagatg ctattgcagt tcatgccatc cggacatccg gcggttcctc
    25021 cgcatccggt tatgcacaga taccggacaa caaatctttt tccaatgtaa atggtgcgga
    25081 acttttgatg actccatcta aggctatcgt atctgccgat caggaaaagc agacctgtgt
    25141 ctatctggat acagatggaa atgcccttat cacagggaag aagatttgtc tccatgcgga
    25201 aaagaatctg attgtgggtg atcctgcaga ggaagacgga caggcttcca gacaaatggt
    25261 gctggaggcg gaggaactga cggttcaggt cggagggaac ggttctcaga tcaacttaac
    25321 ggatgaggca cggattatag cagcctttgt aaagatggat gcatcggacc ggtcgcctgc
    25381 tgcaaatccg tctctggaag aactcagatc atctgtaaca gcaaatgatg agaccaacag
    25441 aaaaaatatc aatgaagatg tcagcaatga gctggtggag aaattcgagg aaggcagaac
    25501 cggtatcctg aaagggatcg taaaagtggc tgccacagtt gcaactgtgg cggtagcggt
    25561 agcgctgacc gcagcaaccg gcggtgctgc ggcagttatt attgcaaccg tggtgttggg
    25621 agcagctacg gctgcatatg ctgctgcaga tgtgggagaa ggaatagaca attacaaaaa
    25681 ggcccaaagc ggtgatttaa gtcagggaca caatttcatg cgggatgacg tattcggtgg
    25741 aaacgagctc ctgtatgaac tggtgagagc gggtacggag attgccttcg gtgttgtatc
    25801 cggctgtgca ataggtagtg catttaataa attgatgaca gcaaaaaaat tcatacatct
    25861 tgctaaggaa gcaaaatgtc tgaaggcagt catgcagata gggggaaatg tcctggatgg
    25921 tatttttgat gatctggcac ggacaggaga gatcaatcct gccaggacac tatggaatat
    25981 cgggatagga tttgcacagg gcagtaccgg cagtggcatc cgtgacggac tattgagtaa
    26041 attaggtatt gaaggatgtt ccttcggaaa ccatctcatt aagacatttc tgggcggttt
    26101 tattgacacc ggcatagatg gggtgggttc tgctctgagt gaacaggact ttgatctgaa
    26161 gagcagcctt atccagaatc ttttctttaa tggactggac gcttttatca gcgatcctgt
    26221 ggacgcagtc acgggaatct atgtcattca ggctacggat ttcctgctgg ccagcgtgcc
    26281 ctttgccctg aaactggaac ggtcctatta ttctaccaat aatacggtct ctgtactggg
    26341 gctgggatgg aaattcccat atgccagcag gatatatcgg gatacaaggg atgtggagca
    26401 tacccgggtt catctggaga ccatcacggg tcattctgtg tgctacgagg agcaggatgg
    26461 ccgttgggtg aaccagagca agggagcatc cagattcctg atggaagtac aggaggcaga
    26521 gataaccgga caggagcggt atgtgcttac ggatgtggtg gaccataccc tgagtgttta
    26581 tgatgccaga ggactgcttc agtctgtgga atatccaaat cagcagcgct taagctttgc
    26641 ctatggagaa gaggggctgg aacggatcgt tacgcccctg ggcaatgtac ttcaggtaga
    26701 gtgccggggt gggcgtattc tgcagattac ggatgagatc ggacgcagga cccagtatcg
    26761 ctatgagggc gatcttctgg tggatgtggt gcatacggac gaggggatta cccactacga
    26821 atatgatgaa aacggacata tttcttccgt tacggatcag aacggaagtt gttatctgga
    26881 gaacgagtat gacgtgaagg gcagggtcac ccgtcagaac ttttcaagcg gtgtgtacca
    26941 gacctttact tatgatgata ttcatcacag gaataccatt tattacagcg aaacgggcaa
    27001 gacggagatc tatgaataca ataaccggtt tctgaaggaa cgggtaattt atgaggatgg
    27061 ttcgtttcag acctatcagt attcggatga caacttaaag gtggaagaaa tcagtcgtct
    27121 gggcagcagg aaagagtggg agtacgatgc ctatggaaga agcatccggg aaaaattccc
    27181 ggatggcttt gaagtgcacc atgaatataa tgagcaccat gatctggtac atacatggga
    27241 cagcgacgga agagagaccc tctatactta tgaccaggaa cacaatccgg tcaggacgat
    27301 ggaaaagatc aaagagggca aatggaaaga aacggcccgg aagtatgatt tcaggggaag
    27361 atgcattctg gagcgggatg ccctcggaaa cgaaagcctt aaggaatatg aagccagccg
    27421 ggcatacccc agccgtgtca ttacgccgaa gggggaggag actgcctacg gctacgat
//
LOCUS       DAWEJW010000027        26108 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_781_length_26108_cov_56.661191, whole genome shotgun sequence.
ACCESSION   DAWEJW010000027 DAWEJW010000000
VERSION     DAWEJW010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 26108)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26108)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26108
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_781_length_26108_cov_56.661191"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2538..2637
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6853 a         6489 c         5149 g         7517 t
ORIGIN      
        1 ccgatatgag cataaaagtg ggatgataat cccacgatat gcgaatattg ggtagaattg
       61 tgagagtgcg tagcacgaag caattcgtag gcatcaaagt cgggggcttt tgaccccgac
      121 atcattttat tctatttctg tcaagaaaga aaggaaatta ttatggcaaa gaatcctatt
      181 gttactatta ccatgaagga tggcggtgtg atcaaatgcg agctttatcc cgaagttgca
      241 cctgaatccg ttaacaattt tatcagcctg atcagcaagc atttctatga tggtctgatc
      301 ttccaccgtg tcatccgcgg tttcatgatc cagggcggat gtcccgatgg caccggtatg
      361 ggcggccccg gctacagcat caagggtgaa ttcgctgcta acggcgtgac caatgatctg
      421 aaacataccg aaggcgttct gtctatggca agagctatgc atcccgattc cgcaggcagc
      481 cagttcttca tcatgcacaa gacaagtcct catctggatg gctcttatgc cgctttcggt
      541 aaagtcattg agggtatgga tgtggtaaat aagattgctg agacccctac cgattacagc
      601 gatcgtcccc tggaggacca ggtaatggaa agtgttaccg tagatacctt cggcgagacc
      661 tatcccgaac ccaacaagct ggatcgataa gatttcaaga aataacctcc ggaagtcaga
      721 tgttctctga tctccggagg tctttttatg tccatagctt tcatatctat ggatcagtat
      781 tccacatttt cacagttttc aataaaaata ggatcttgca cttcgccctt catgtcctca
      841 gtaatctcat aggagaaatt ccggaaatgg atattttccg cattgcgggc atacagcact
      901 ctgagagaat cctccaggat cacattcccc tcattgggac gcagatcatg gcaattcttc
      961 ggccagtccg tcttcttctc cagttggaca tgaataccgt caaatgtgat atcccggata
     1021 tttttcgaat cgtcaccgta gatcaggatg ccattctctc cgacacagtt gatattctgg
     1081 aaacggacat tccggatata tcccacattc gtctgtgcat ttctcttcac cgcagtgatg
     1141 gtaatgggtt ctgcctcgcc ccaccaatgt accttggaga acaggcgggt atcgatattc
     1201 agattggaga atgtcacatt ttctatacag cctttatcac gtaactgcag ggaaattgca
     1261 cggttggtgc gctggatact acagttggtc acggaaatat tccggaaagg tgcttcactc
     1321 tccgtgccga acttgatagc ggcactggtg gagatcatgg tacagttatc cactacaata
     1381 ttttcgcagg gaccatattc catggcagcc gccgtattct taaatacaat acagtcatct
     1441 gcgcattcaa tatgacagtt ggagattctc acattgctgc agtgatccgg atcaatgcca
     1501 tcgcagttag ccagacgcag attattgata atacggattc cctcaatcaa tacttcctta
     1561 catcccacca gatgagtggt ccagaatgca ctgccggtca gggttacccg ctggatggtc
     1621 agatgcctta cattttccag gaacagcaac ggtactcttg gataaaaata tccgtcaata
     1681 tgccatttgg tcacttttcc atagaatatc tcctcgttgc cgtcaatctt gccaaatccg
     1741 gtgatagcca cattctcggc atccttgctg tatacaaaat tcagcgtcgg ttttccattg
     1801 taatcacagg cattgtaagt cggagtgtcc accttgctga tctgtggggt tcccactttc
     1861 aacatatcga aatcttccag atggtcgctg gctttcagga cagcccccat ctccagatga
     1921 aattccacat ttgatttcag aaaaatggtt ccgctccgga agacatgtcc tccctgtaac
     1981 aggaccttac cgccgccagc ctggctgcag gcatcaatcg ccttctggat ggctgcagcg
     2041 tcattcgtca cgccatcacc ttttgcaccg tattccaata catcgtatat catctctctg
     2101 ctcctctctt agcccttcag gccggaagtg gaaattcctt ctaccagata tctctgcagg
     2161 cagaagaaga tcaatactgc aggcagaata gacaaggttg ccatagcgaa catagcaccg
     2221 tagtcggaag aagaactggg gtcacagaac attttcagtg ccaggctgac aggatatttc
     2281 tctgtcttgt tgacatacaa taatgctcca aggaaatcat cccatctcca cataaaggag
     2341 aagatacatc cggtgatcat ggtaggaacc atcagcggga agatgatctt ggtgaaaatg
     2401 gtataatagg agcagccgtc aatctttgct gcctcatcca gctctctggg aatgccttca
     2461 ataaagtttg tcatcatata aacgaagaaa ccctgaatag caaagcaata aggcacgatc
     2521 agcggcagat tggttccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga
     2641 agaaaccctg aatagcaaag caataaggca cgatcagcgg cagattggtt ccaacccagt
     2701 tcagcttctg ataccagaga tactgaggga tcatcagtac ctgtgccggc agcatcatag
     2761 atgccagcat tgctgcgaag agaagctttc tgccggggaa tttgcatctg gcaaatccat
     2821 aggctaccac agaggaagaa agtacggttc caatggtggc ggtaatggag ataaacagag
     2881 agtttttgaa gaaggttcca aaacctacct ttgcaaaacc cttccagccg tttacatagt
     2941 tctgtaatgt gaatttttcc gggatcaggc tggtggctgt ggtaaagatg gtgttcgtat
     3001 ccttaaagga actcattacc atccatacca gcggatagat cattaccagt ccgaaggcac
     3061 atactaaaat gtgatatata atattgctga ttgtcttttt tcttttcata ataacaccca
     3121 cttacgcgtt gcggcgtacc tttccgattc tataaaccgc catcgtatac ccacagtttt
     3181 ttggttgcaa acaggatcca tgtaataaat gcgatcaatg ccagcatgat ccatgccagt
     3241 gctgcgccgt aaccggtact tccgaagagg aaggactgct ggtacatata taccgtgtag
     3301 aacagagtag agttcatggg tttaccctgt gtgatgatca ggcactgtgt aaatgccaga
     3361 aaaccgttga tcatctgcat taccaggtta aaatggatcg tgggagtcag aagcttcagt
     3421 gtgatcttcc agaactgctg ccatccgttg gcaccatcca ctcttgccgc ttcatagagg
     3481 ctgacaggaa tctgcttcaa tgcagacagg aagatcagca tggaagaacc gaactgccat
     3541 accgccagaa tgatcagagt ccagattgct gttctggtat cacccagcca tgctaccggc
     3601 tctttcattc cgaacaccat caaaagttta tttacaagtc cgtctaccgc aaacagtctc
     3661 ttccagagga ttgctactgc tacagatccg ccgatgatgg aaggcagata atacagtgca
     3721 cggtaaacgc ctgtcatttt cgtactctta aataaaagca gtgcaacgat caatgcgaag
     3781 atcagtcgta acggaaccga aatgatggca aatttgaaag ttaccaccag agactgataa
     3841 aacgttgcat cagaaaacat tctcttaaaa ttcgcccatc cggtaaatgt agggggctgt
     3901 aaaatattgt aatcgcagaa ggaatagtaa agcgatatac acatgggaat aatcataaac
     3961 gccagaaatc caacgataaa tggcatagta catacgatac ctgcagcctt ttccgtattc
     4021 agcttctgac ggatagtcat gtgctttgtt gtcttcataa cgttacctcc gttgctgcag
     4081 cattcataga agcagccttt tttcagaatg ggtactgcgg atgcttccac agtacccatt
     4141 ttgtcattct aaaaaatgtt gtaagattgt tattacttat gtaagttcag acagcttatc
     4201 tggacatgat ctggttgccc atcttgaaca gatcttctgc tgcctcctgt gcggtcatct
     4261 gaccatagag gatcttatca accagctggt tgtagtaatc atatacttca tttgctccat
     4321 cagggatcgc agggctgata gcggagcagt tgggagttac tacgtcattt acatacttgg
     4381 taaccacctg tgcagtctca tcctgcagag gagcgatggc ggatgctact acggaagaag
     4441 caggcacacc tctctcacca agaaggatct tgttagcatc ttcagagttc aacagatagt
     4501 ttacaacctt aacagcttct gcctcattct cgccggcatc tctggatact gcaaagaact
     4561 ggctgggctt taagaagtta gacttctgag ggttctcgga aggccatgtg gtgatgccga
     4621 tctccatgcc ttcgggagcg gccgctacca tagcagacag ctggttggac cagaagaatc
     4681 cgcaccagga ctgggttgct tcggaggaga agtataccag aggactctgc tctacagagt
     4741 tggaagtcag ttcagcgtga acatctgcgc cgatgatcca tccgtccttg acaccatctt
     4801 cataaatctg gaagcccggt accagacttg ctgcatctgt tacattgagg gaatcctcat
     4861 tgaacagctt caccacatca tgagaacgca ttgcataggg cagatagttg tcacctgcac
     4921 tgtaaggaag atcagtctta acaccggact tctcataaac ctcacgtgcc actgcctcga
     4981 actcatcaat tgtcatgtta tcttttacag tgattcccag gctgtccagc aaggtcttgt
     5041 tgtagaacag ggaaggtgca ttcattcctg cacagactgc atataactta ccgtcaatgg
     5101 aacccgcatc cagaataccc tgagatacat tagatacatc cagcgtacca tcttctacat
     5161 aaggtgtcag gtctaccagc agatctgctg ctacatactg tgccagatag gaatagtcca
     5221 tctgcaggca ctcgggaagg gaattacctg ctgccagggt ggacagcttg gtccagtaat
     5281 cattccactc tgcaggctgt gtatcaaagg ttacaccggg attctgctca gaatacagat
     5341 ccagggctgc ctgtgtacgt tcatttctgg tctggttgcc ccaccaggtg aatgtcatgg
     5401 atacatttcc ggccgctgca cttccgctgt tgtcagccgt cgtggttgcg gtattaccgc
     5461 tgtcggtgga agcggtagct gcgggttcat tggaagaacc acatgcagtc agtccgacga
     5521 tcatggcgga tgccattaac agggataaaa ctctcttttt cattaatata accctccttt
     5581 tggtatcgaa aggtaaatcc gctatggatt tacttctttt tgatgttcta attataacaa
     5641 ttcttatttt ttccaggaat atacttttcc gtctatttgc accaaaaaac cgatatcatt
     5701 gttgaaaaca tgtgcatgtt gtgtatataa tcaataaaga actgatggag gcttgtatat
     5761 gatgtcattt tttaataaga atatctcgag aaaaatagca aatcagaagt tagaaaccaa
     5821 gttactgctc tctaccatag ggattgacct tctctttctg ttctttttcc tggtagctgc
     5881 cttttccatt attacatcgc gttatcataa gctgctgtat cagtccatgc agtcttcctc
     5941 gtcgttagtc tcctatgaat ttactaaccg gctggaggat ctggtgacca tgaccaatat
     6001 cgtccgttca gattctacag tccaatcaac actggatgaa atctaccagc cccaggaaga
     6061 ctatgctgta cattattact ctgatattta ttctgcttta cagaaacact acctggaata
     6121 ccgccagccc tacctgaaaa tggcggcaat ttcctgcccc agattcatca cttataccaa
     6181 tgaaaatatt gcctgtcggc cggatgcgga tctgactaaa gaactgatag ctctggcaga
     6241 ggccggggaa ggctccccgg tatgggtaac ttcccatgcg gaagaccacg gtatatttct
     6301 tgtccgggag atcaaaaaga tcaagaactt acgactggat aatctcggaa cattgatcat
     6361 caatgtaaat ctgggagatc tggtaacgga aatgtccaag atcagcaatg aatataaaga
     6421 ttcctactgg atcatctatg agaaagacca gctgctgttc tcttctccgg aattggatac
     6481 agaggaagta cagaaaatca atgataaaat caaaagttat gctgttgtca ccctgaatgg
     6541 cagaaaatat tttgccatcc gcggtaccat ggacatcaat gaatggaatt atctgcatct
     6601 gatctcttat gacgaagtgg caaaatccca gcagatgact gcatttctat atgtattgat
     6661 cctgttctgc ggtttcctgt gttccatttt gatcgtccac ctgatcgtac gtaagatcac
     6721 cagacatttt gatctgctgc tctaccgtat gcagcggttc ggtgaagact ccaccgtact
     6781 ggcagagatt ccttatgatt ataatgatcg taccgatgag attggtattc tgcacaggca
     6841 atttacacat atggcagagc ggatccagac actggttgtt gaaaattacc gtcaacagct
     6901 gctggctaaa gatgcgcagt taaaaagtct ggaatcccag atgaatccgc attttctgta
     6961 taacacgctg gatgccatca actggcgggc caaggccatc ggagagtcgg aaatatcaca
     7021 gattgcagac tccctgggtc attttctgcg gatgacgctg aacaaaaaag aacgctttac
     7081 gttgaagcag gaacttgata ttatacatta ttatatgact atacagcagc ttcgttttga
     7141 caaccgtctg aattttacgg aaagcattcc ggatgcttgt ctggatgcac aggttcccaa
     7201 gctttccatt cagccgttgt tggaaaatgc cgtacactat gcgctggagc agatcacgga
     7261 tgaatgtatc atcaatctca cctgtgtgta ttcccagggg ctgatgcaga tttatgtgaa
     7321 aaacagcgga tctgaattcc ccgatgattt attggagaat ctccggacac ataagatccg
     7381 ggaacagggt ctgggcattg cgttgttaaa tattgaagaa cgcatccagc tgatgttcgg
     7441 ccagcaatac gggctgcact tctataacga aaatgatttt gctgtagtaa aaatggagat
     7501 accttatgtt aaagatgatt atcgtggatg atgagaagat catacgtgaa acgatccatt
     7561 ccctgatcga ctggaattct ctgggaattg atgtggctgc tgtctgcaaa gacggtattg
     7621 aagcctttga ctgtattctg gatgaatacc cggatattgt catgaccgat atcaagatgc
     7681 ccggtttatc cgggctggat ctgattgaaa aggtccgtgc tgcccagttg aacacagagt
     7741 ttatcatcct ctcaggatat ggtgaatttg aatttgcacg gaccgccatg cgctatggtg
     7801 tgaaacacta tctgttaaag ccttccaatg aaacagagat cacgcaggtc atcgtatcct
     7861 gtgtggaaac ctgtaaaagc aagactgctt cccgggtaga ttccttgctg acacagctgt
     7921 tcctcacgga aatgccaccg gaaaaacttt tgcagagaag atttttcaca gaattatccg
     7981 gggaacagga tgtggatctg gccaagaccc agatgatccg tctggtaatg gaggcctcca
     8041 agaaggaata ttacccgctc agcaaactgc agaccacaga cagtctcatg gcagtcaacg
     8101 tctgtgtcac catgaatgac ctgattccat ctgcggagca gatcatgacc tcgatcttct
     8161 ctgtggaacc gcagcacaaa tacacagact gtgtggaaaa ggtcataaag tacattggag
     8221 agcatctgag tgacagcaac ctttcactga aatggatctc ggaaaattat ctttttatga
     8281 atccggatta tgtcagcaaa atgttcgtca aacagactgg cagcaaattt tccgcctatg
     8341 taacagagct ccgcattcag gaggcgaaaa aacttttact ggagcactca gaagaatctc
     8401 cctatgcggt agcggaaatg gtcggtttcg gcaataatcc ccagtatttc agccagatct
     8461 ttaagaaata tacgaggctg tcaccgaagg attatgtgaa gtcaatgctg gaaccgtaag
     8521 accgcagcta ttgcagctat tcagaacccc attcgcaaaa taggccgttg ttcatatttt
     8581 gtttacaaat ccttcaagga atcttcaatt ccataacacg ttttggacat ttctgtttca
     8641 tatactataa ccatagaaac aaacaaaaca aaaacttaag cacacaacag tttcttgttt
     8701 aaaaaacttt ttcataataa atgccaagga tcctgatcct tggcatcctc cctttctagg
     8761 ggttttttca actatcacag atgctatttt gcaaatgaag ctctgaatag ttattttttt
     8821 gtcttaatgg tcggaatcgg aataaacaag aatctcgctt gcgagattct ccgcctgcgg
     8881 cgggtcgctt ttgcgacata cttcataacc gccgcagtgc gatccccgcg gagctttttt
     8941 aattcatagg aagaaatttc ctatgaatta aaaaagtctc ccgcatcgct gcgagagact
     9001 tttgcatttt tattccagaa ttattcagcc ttgccaacag atccgaacag ttccatcttc
     9061 tccttaacag tagccttgat agcttctgca ccgggagcca gaagcttacg agggtcaaat
     9121 cccttgcctt ccagatcctt accagcctcg atgtacttac gggtagcatc agcaaatgcc
     9181 agctggcact cggtgttaac gttgatcttg gatacgccca ggtcgatcgc tttcttgatc
     9241 tgatctgcag ggattccggt accaccgtgc agaaccagag gaagctcacc ggtcagctcc
     9301 tgtacagctg ccagagtctc gaagcttaag cccttccagt tagcggggta tttaccatga
     9361 atgttaccga tacctgctgc caggaagtca acacccagat cagcgataga cttacactcc
     9421 ttaggatcag cgcactcgcc cataccgata acgccgtctt cctcaccacc gatggaacca
     9481 acttctgcct cgatagaaag tcccttgctg tgagcagcct taaccaactc agtggtcttc
     9541 tctacgttct cagcgatagg ataatgagaa ccatcaaaca tgatggaaga gaaaccagct
     9601 tcgatgcact tgtagcagcc ttcaaaagag ccatgatcca gatgcagcgc aacgggaaca
     9661 tcaatgtcca gatcctttaa cagtccgttg accatgccaa cgactacatc ataaccgccc
     9721 atgtacttgc cggcaccctc ggatacaccc agaataacag gggaacgaag ttctttagcg
     9781 gtgagaagga tggactttgt ccactccagg ttgttgatgt tgaactgacc tactgcatag
     9841 tggcctgctt ttgctttttt gagcatttct gttgcggata ctaacatttt aatacctcca
     9901 ttttatatac tcattcttgt tacgatatac gaaatgttat acattcgtaa aagcacgaaa
     9961 aacacatttc gaataatcac tttgtgacta tccaaactat agcacattct tttcaaaaag
    10021 ggaacagctt attgttaaat ttttaccaaa ttttaagatt ccttctgttc cacccggatg
    10081 tctacgcccc ggcagatgtt ctcaatcact acttttgtat gggttccgcc atattctcca
    10141 tccagagtcc atggaagagg ctctgcggaa gtgatctcca cctttgacgt ccggaagcag
    10201 tacatagcat cggtatcgat atcccggttc agcagggata ccataatgtt attcagttca
    10261 atcgggttct tcggacgttt gatcagcgtt acttcgaaaa cgccgtcatc cagcctgacg
    10321 tttttgccgg tgatccgctt aaatcctcct accgatgcgg aattggtcac cattccgaag
    10381 ataaaatcat ccacgatcgt agtatcgcca taagtaatcc gcatgggata ggaacggatc
    10441 gcggacagcc gcttcattcc ttccagcaga tatgccatat gccccagcac atttttcata
    10501 tcctgtccgg tctcataaga gacatccgtg aatagtccga aagctgcgat ataaacgaac
    10561 acatcctcat taaaggatcc cacatcacag gcgaaatcat tcccatttac aatattttcc
    10621 gcagctctca ccatattctt cggcagcccg aggcttcctc cgaaatcatt ggtgctcccg
    10681 gccgggatat agccaatagg cgtacggaag ccactgcgga tcatgccggt gaccacctca
    10741 tccagcgttc cgtctccgcc gctgcagacc acgatatcat attctttgct gcgcttctcg
    10801 gtcttctcca tggcatcctc acttctctgc gtgggatata cggtgatctc ataaccgccc
    10861 ttggtgaata catccagaat atccgccagt ttgttgcgga tttgtgcctt tccagctttg
    10921 ggattataga taaataacat cttcttcata atactgccct caagatacaa caaacgggac
    10981 taacagaatt ctccatcagc cccgcttctt ctcatgttat gttcttaagc cacctgaccg
    11041 ctcaaaaact tttcaatgtt gtccacagcg gtctgctcat ctgcaccctc tgcagttact
    11101 gtaagctctt cgcctgcaca aagtcccagg ctcatcatac ccataatact cttggcattg
    11161 atccgcttat cgtctacttc aaaataaatg ttgctttcgt acttgctggc ttcctgtact
    11221 aacagtgcca ccggtcttgc gtccaggcca tcttccagtc tgaccgtaat actcctcttt
    11281 gtcataattt tcctcctctt ttacgtccgg attctatctg ccagttcgct gagtttacgt
    11341 aatctatggt tcacaccgga ttttcccacc ggcggatcca gatactctcc cagttccttc
    11401 agcggagcgt ccggattctc cagcctgacc tctgccatct ggcttaaggg ctcgggcaga
    11461 ttctccagtc cgtagtgatc ccggatataa ataatatcct ctatctgtct ggtcgctgca
    11521 gttaccgttt tggtaatatt ggcggtctca cagttcactc tccgattcac agaattgcgc
    11581 atttctttca caatcctcag gttttccagc ttcatcaggg caatgggtgc ttcgcacaca
    11641 ttgagaagat ccacaatacc ggatccttcc ttcagataga ccacgtaata cttcttccga
    11701 agcacaatct tggcctctat gtcaaatccc tgtatcacac tctgtagttg tctcgcttta
    11761 tcctcatcgg tgcatacaaa ttccaggtga taacttttct gcggatcgct catagaacca
    11821 atgcagagaa aagctcctct taaaaatgct ctttgacagc aggaattttt aatcagcagg
    11881 gggctcacag gctcagacag atccccgatc ttatcgatca cactccgcat ctgttcctcg
    11941 ctgattttaa catcagtatc tatattatat gtttttttca ataatgtaaa gccttttctg
    12001 acaaccgcct cgttttccgt ctggaaaccg atcatgaaat ctccttctgc ggagcgtccg
    12061 tattgtccac tgaagttcat gagtgcggcc agctcagcga tctggcaatg tcttgccggg
    12121 ctgatgtgtt ttgccagttc ttcctttact tcactcgaaa atgacatgtc agattccttc
    12181 tctgggtaca tcacggtgat acagcttgat accataatga ccttccgcct tcatacgttc
    12241 gtacaattta tttgctaaag taacgcttcg gtgcttaccg ccggtgcagc cgattgcaat
    12301 aaccagacgg tatttgcctt ctttaatata attcgggatc agaaactgaa tcatgtcggt
    12361 cagcttgtcc aggaatattc cggtctccgg aaaactcatc acataatcct gcactccttt
    12421 atcattaccg gtctggggct tcagttcctc gatataataa gggtttggca ggaatcttac
    12481 atcaaagacc agatcggcgt ctgcggggat tccatgcttg aacccgaagg acatgaccgt
    12541 taccataaga ctattgtatt cttcgttctg tacaaaaata cgatccagct cttccttcag
    12601 ttctctgact aacagattgg aggtatcgat gacgtaatct gaatttttgc ggattgtctc
    12661 caggatcttg cgctccttgt gaatgccgtc ctccactctg ccgtctgctg caagagggtg
    12721 cacacgtctg gtctccttat accgttttat cagagcagca tcgtcggagt ccataaacaa
    12781 aatctcgaat ttgtagcctt tttcctttaa ttgctccaaa atccgggtcg catccatgaa
    12841 attctggtct gcccggacat ccagtcccag ggccaccttg ctgatctcac tgcccggcat
    12901 ggcaatcagt tctacgaact tctccaccag agataccggc agattatcca cgcagtaaaa
    12961 tccagcatct tccaaaagct ttaatgcggt tctctttcct ccgccactca tgcctgttac
    13021 tactacaaat ctcatatgca cctcgttatt ctatcgtaac tattcagaat cccattcgca
    13081 aaatcacctc ttcgaggctt cctttttgct catgtggtta cttcgccata taaatttgcg
    13141 attctgcgaa gatgcaagca tccgcagaaa acaaatttac atggttctga atagttacag
    13201 gaatttgatc tcgggttcca gctccacgtg aaaacgttct ttcactcttc tctgtacttc
    13261 ccagatcaca tcttttacat cttctgcagt ggcattgccg gtgttgataa taaatccgca
    13321 atgcttttcc gatacctttg cgcctcctac ggagtatccc cgcagaccgg cttccatgat
    13381 cagctgcccg gcaaaatgtc cctcgggacg tttgaaagta ctgccggcac tgggatattc
    13441 caaaggttgt ttttctctgc ggcgaattgc cagttcctcc attttggctg cgatctgctc
    13501 cggatctccg ggctgtagcc ggaaagtcac tttcgtcacg acaaacgact ggttctggat
    13561 cacgctggta cgatagccgt actccatggt actgttgtcc agttccatga tctctccatt
    13621 acggttgact accgtaacag tggtcaccac ctggctcatc tctccgccgt aggctccggc
    13681 attcattaca gctccgccac cgatggtacc ggggattccc gcggcgaact ccaggcctgt
    13741 caggccatgc tctttcgctg cttttgccac ctggctcatc agggcccctg cctctgcttc
    13801 cagatagcag tctctcactt cgatccggct catatgctta ccaaccacca gaacaattcc
    13861 tgcatatccc tggtcgctga ccagcagatt gcttccgttg ccgatcacgg tatagggaac
    13921 atccaccagc cgaaggtatt tctggatctt acagagctgc tcttcgtttt ccagatagac
    13981 caggcaatct gccgggccac cgatccggaa ggtggtatgc aatttcatcg gttcctgtaa
    14041 cagaacttta tctgccggta caatttcttt taatgcttct atcactgctg cacttatcat
    14101 gttgtcaggc tgctctttct ttttgttata attatgccgc gaaagcctac tttaatacca
    14161 gtgctgccgc accgtagatt ccggcatcat ttcccagctt ggccagggcg aatttcgtct
    14221 gcttgcaggg accgaaagca catctcttat aagaaggctc tatgtatttg aataaaatgt
    14281 ctccggcctt ggacacacca ccgccaatga caaaaatctg gggatcaact acgctggcta
    14341 ctgcggcaag tcccttgcca agatactcgc cgaactgttc tgcgatctcg attgctactt
    14401 tatctccggc tttcaccgca tcaaatacgg tcttcgcaga gatttcacca cctctgagca
    14461 tgctgggagc atcgtctttg gccagacgtc tgttggccag acgggtaatt ccggtagcgg
    14521 atgcatactg ctccagacag cccttgttct tacagccgca gacctctgtc tcgttgtctt
    14581 cgatatgaat atgtccgatc tcaccgccgg caccattagt accagtcagt atcctgccgt
    14641 cgatgatgat acctccaccg acaccggttc ccagagtaac ggctaccaga ttggaatagc
    14701 ctttaccacc gccctgccac atttcaccca gggctgccac attggcatcg ttacctgcct
    14761 tcacggtaac gggcagatcc agttcctttt tcagcgtttc agcaagatca aaagatcccc
    14821 aaccgatatt aacagcaccg ttgaccatga aaccttcggc atttactgca ccgggggcac
    14881 cgattcccac acccaccaga tctgctgcgg tgatgctctt ttcctgcatt ttatttttga
    14941 tggatgctgc gatatctggc agaactttct ctccgccgtt atcttttact gtgggaatct
    15001 cccacttatc cagcacctgt ccctcatcat tgaaaagtcc taacttgacc gtggttcctc
    15061 ccacgtctac cccaaatgca tatttactca tcatcctcgt cctcctcacg tctttttgcc
    15121 agagagttct gtacacgggt gtatagctct tttgctgcgt tgtagcccat cttcttctga
    15181 cggtggttga tagctgccgt ctcgcagata actgccaggt tacgtccggg acgaatcgga
    15241 atgttgtggc ataccacctt attgcccaga tattcggtat aattctcttc cagtcccagt
    15301 ctgtcgtact ctttgtcctt atcccaatct tccagacgga tgaccaggtc aatggtctgg
    15361 gtatctttta cggacaatgc accaaacagt gccttgacat ccacgatgcc gataccacgc
    15421 aattcaataa agtgttttgt gatatccggt gcagaaccta ccagcgtatc atcactgacc
    15481 ttacggagtt ctaccacatc atcggttacc agacgatgac ctcttcggat cagctccagt
    15541 gctgcctcgg acttaccgat gccgctctca ccggtgatca ggacaccttc accgtagaca
    15601 tccaccagta caccgtgcac ggaaatgcag ggagccagct tgacatttaa ccaccggatg
    15661 atctcagaca taaagtcaga agtggatttt ttcgtagata ataacggaat ctgatgtcgg
    15721 atcgccgtct ccaggaaaat aggatccggt ttcaggtcac ggctgaacac gatacaggga
    15781 atatcataag ccatcagctt ctcataagct tccttacgag tctcatccgt catgccttcc
    15841 atataagtgt attccacaaa tccgacaatc tgcagtcggg tcgcatcaaa atgctcaaaa
    15901 tatcctgcca gctgcagtgc cggacggttg atatccggct gtgtgatctt cacatgtttc
    15961 acatcaatct ccggtgtgag attttccagt ttcatttttt cgataacacg ggttaaactt
    16021 acacttgcca tactaatctc catacctttc cgcgaaaaaa ccgccccggt tccccgccct
    16081 tttcattatc ttttagtgta ccacaggttg ccctgtctgt gaagcaaaaa aggccacgat
    16141 ttcctctgcc gtacgctcat tcatttcagg aatttccatc aattcttcca ccgatgcctg
    16201 ccggatctcc tccagtgact taaagtgcct catcagggcc tttctccggg ccggtcccac
    16261 ccccgggata tcatccagta cggacttcac ctgagtcttg ctgcgcaggg atctgtgata
    16321 ctcgatagca aaacggtggg cctcatcctg aattctggtg atcagcttaa aaccttcgga
    16381 atgggtatca atgggaagct caatattatg ataatataag cctctggtac gatggttgtc
    16441 atccttgacc atgccgcaga ccggaatgtc gattcccagt tcttccagta cggacagcgc
    16501 aatattcacc tgtccgcgac cgccgtccat cagaatcaga tccgggaact tcgtgaagct
    16561 tccgtactcc tgatccatct cctgttcttc cagctctttg ctctcttcca tgccgtggcg
    16621 gaaccgcctc gtcagcactt cccgcatgca ggcataatca tccgggccgg agacactctt
    16681 gattttgaat ttacggtaat cgctgcgctt gggcttcccc ttttcgtaga ctaccatgga
    16741 gcctacattt tcaaaaccgt tgatattgga aatatcgtag gcttccatcc gggccgtgcc
    16801 ggtcagcgga agctgcaaca gatctgagat ttccttcacc gcaccgatag tacggccttc
    16861 ctcacgcttc agtttctccc tgtcctgaga caataccagt tttgcattct gggctgccag
    16921 ttccaccagt ttttccttac tgccgatctt tggcaccttc aggtagaccc tgctgccttt
    16981 ccgttcagac agccattttt caataagttc tgcgtcctca atctcatatt gcagcatcag
    17041 ctctctggga ataaatgggg tccccgcata gaactgcttt acaaaatcct gtaaaatcgc
    17101 tggtttgtta ttttccggca catgtgtcat atagtaatgt tccctgccga tcagcttgcc
    17161 atcccggacg aaaaacacct gtaccaccgc ttccgtctca tcctgataca gtgccaggat
    17221 atctttatct tcaccgacgc cctcggtgat cttctgtttc tgggacacct ggcggacact
    17281 ggataacaga tctctgtatc tggcagcttc ttcaaattcc agttcctccg ctgccttgtt
    17341 catcttctct tccagatcct tcagaatcgg gctgtaattg ccgtttagaa attccagtgc
    17401 tcccgccacc tgctgtctat actcctcctt gctcacatag ccctgacagg gagccaggca
    17461 ctgtttaata tgataattca ggcaggggcg ctccagtccg gtgtctctcg gcagcacccg
    17521 gttgcaggta cgcagctggt acagcttgtt cagcagctca atagtatctt tgaccgccgc
    17581 agcactggta taaggaccga aatagcggga cttatccttc ttcatggtac gggaaaacag
    17641 gattctggga taatcctccc ccaccgtcac cttgatataa gggtacgtct tgtcatcctt
    17701 taacagcgtg ttatacttcg gtgaattctc cttgatcagg ttattctcca gcaccagtgc
    17761 ctccagttcg gagtccgtca cgatatattc aaatcgcgcg atcaatgata ccatctggtc
    17821 aatggcagga cctctgccga tattttcacg aaaataagac cttacacgat tgtgcaggtt
    17881 gatggctttt cccacataca aaatgacatc cttgtcatcc cgcatgatat atactcccgg
    17941 cttgcggggg agctttttta attcttcttc aaagttaaac ataaatattt cctcgcatgc
    18001 ttctatcata ccgattcttg agaaaaatac aacaagggat ttttatcttg tacaaaacaa
    18061 aagcggtgtc tgccagcaac tgcaaacacc gcaaaggaaa ggtaatatat ggtatcagcc
    18121 ttttacactg ccacttgtca gacctgccac aaagaatttc tggcagcaga taaacacgat
    18181 aagtatgggt aaaagactaa atactgacaa tgcaaagaca tagccccact gtgtaaattg
    18241 atgttctcca aaaaatccgg agattgcaat tggcattgtc cgtttcaaat tatcatttaa
    18301 aaccgtattc gcccaaggaa attcctccca tgcagtcaaa aaattgaaaa tagataccga
    18361 agcgattccg ggttttgcaa ggggaattat gatccttccg aaaaccgtaa aaacacttcc
    18421 accatccata taagttgctt catccaattc tctgggaatc ccttccacaa aaccctttat
    18481 cataaatgtg gaataaggaa ttgtccatgc tgcataaaaa ggaaccagtc ctacaagaga
    18541 gttgattaca cgtaatttta ccgccagttc atactgtgga acgataagtg tcagtcccgg
    18601 cacgatcatt gttaagacga taaatccaaa caggaaattt tttcccggga aatcaaatct
    18661 tgcaattaca tatcccagag ccgaagcaat cgctgctgca atcagcacgg atgctacgga
    18721 tacacacata ctgtttaaga agttacgata gaattttccc tgtcccaata cgtatttgta
    18781 attttccaga gtcacctctt ctaatgctgg taaaaatcga ggggggtatt catacaatgc
    18841 cccattcggc ttcagcgatg tgctaatcat atataccata ggaagcacaa caatgattaa
    18901 tccggcaatt aatataatgt gaagaaatat ctgtatccat gtttttcttt tcatactcac
    18961 acctgcctat cttattgatc tttctgctgc atgagtttaa actgtattac agcaagtatc
    19021 gcaagtacta cggttgtaat aaaggacaac gctgcagcat agccgaattt tcctgtactg
    19081 aatgccttat gatacatcca tgtcagaata acatctgtct gatgcccggg ttcaccaccg
    19141 gttatcatct taatagaagt aaatacatta aatccgccaa tagtgagcat tactaacgca
    19201 aataaaatcg ttccccggat actcggtatc gtaacattcc acattttatc ccatgctgtg
    19261 gctccgtcga tagcggcagc ttcatagaga tctgtaggca ccgtctgtaa tgctgcaagg
    19321 aacacaacca tattccagcc aattcccttc catattccca gtaacattgc tactgtcata
    19381 ccgccccatc tggtatccag ccatcgtaca tttgcactgg taatatgtac aatattcatc
    19441 agaaaataat ttaataatcc ctctgtgtta aacacatatt tgaaaatcag agacgctact
    19501 acccatgacg taattactgg caaataatac agtacccgat atccgacttt aaaacgtgta
    19561 atctggttca gtaaaagtgc aactaaaaat ccagccagca tctgtcccgg cacagtcaca
    19621 agcgtatata ccaatgaatt tataaaaaca gtccgaaagt attcatctcc caatacttcc
    19681 ttgtaatttt ccaatccaat aaaacgatga ctcagcatac tgccaaaatc ccattcaaag
    19741 aaactcataa taaataattt caaaataggg tagatcgtaa aaatcccaaa tacaagtaac
    19801 ccgggtaaaa gtaataatcc aatctgtatt ttgcttttcc gtgatgtctt catagaaata
    19861 cctcttttac agttgggaca gcacctgacg atactgcccc ggactgctat ttgactttat
    19921 acatatggca agttataatt aatattattc tgcgagaagt tcatcaaaac gctctgcgag
    19981 cttatccata gcttcctgtg ctgttttctt tccgttcatt acatccgtaa ccgctacaga
    20041 taattcgctg tccatttcag accagcaggc tacggtagga cgggattttg cagttttgat
    20101 agcttccagg aacggtgcat aatctgctgc ttttaccgta tcgctttcca gtgcttcctt
    20161 gttgacaggg atctgtccgc attttgccat ttcctcttca gcgaattcac tggtcataaa
    20221 cttcatgaat ttccatgcag catctttatt tgcactattg aacatagcaa tatcttcacc
    20281 acccagaaca gatatggatc cagcatctcc ggcaggcatt tccgtggttg catattcaaa
    20341 atcaggataa gagccttgca tttccgcaac cttccaggga ccttccagaa gcatcatata
    20401 tctaccggtt ccaaaaccat ctgtcatcgg aatgtcacct gcattccatc ctgtgattgc
    20461 acctgcctga tacagttccg caagcgtttc gattgccttt actgtctccg ggctgttgat
    20521 ataaccggat gcctttgtct cctcttcatt ggtaatggat ccacccatac tccagatgaa
    20581 cgggcagaga ttccaacctg ccagtgccgg ctcatcatat ccccatacct gctgaccatt
    20641 tgcattggtt cctgctaatt tcttagcagc atcaacaaat tcatccatgg ttttgggagc
    20701 ctcaatacct gcatccttga aagcctgcac attatagaag agaattttgg tgttggtgtt
    20761 cagtgctaat ccatagaagt gaccatccac ttttgctgtg ctcatagcac tttccaataa
    20821 gccaccggag acatcattaa agtcagacat ctcttcatcc agtgctgtca gaattcccat
    20881 tttctcaaat tcgggaaccc atgcgctatc cagtctggct acatccggta acgtgtttga
    20941 ctgtgcgctg atcagaatct tctgatgcag atctgcccat tcatgagata ctgcatttac
    21001 cttgattccc ggattttctt tttcgaattc cggaatcagt acattcatca gagtttcatt
    21061 ttctgccgac tgcgcactat aatgatgcca gaaattaata gtaacttctt cctgcgaagc
    21121 actttctgta gctgcaggag cctctgctgt tgctgatacc tgactctctg agccagtttc
    21181 agattctact ggtgattctc cacatcctgt taaggctgcc aggctcatta cggttgtgag
    21241 tagaattgcc agtaattttt tgtttttctt taccttcatc attgtaaaca tcctcctatt
    21301 gttttttgct cgagttcgat gaactcatta tgacttttac agaatacggc tccatttgca
    21361 acgtaacttt gcccttgtat tctgttatgt cctcattcaa caattccgct tctccgatat
    21421 cttcctctcg taacagttcc cctgaaagca cttctgcaaa aacgcctctt tccaaaaccg
    21481 gacattctat tttacagttt tttgttcctc tatgcagaat cacataacag cttccagtct
    21541 catctttacg aacaaatcca taagtatcgg ttgtttcgtc tgcaataaca gttctgaagc
    21601 ttccggatcg aatgcaacta taattttttc gcaactgtat cattttttgg taccagttct
    21661 tcacatcttt atccgcattt tcttcccatt ccatacacct tcggcaatca ggatcattgt
    21721 ctcctgtcat tccaacctca tcgccgtagt aaattgcagg ggaacccgga aatagtattt
    21781 gaaatgctac tgccagtttc agaagtgttt tatcttcccg gcacagatac aggaaacgtt
    21841 ctgtatcatg actgtcgatc aggttataca aaagacaatt tgtctcgtcc ttgtaataag
    21901 ccagcatatg atttagtcta ctgtcaaact ctgttacgga aattgacttt tctgctatgt
    21961 aatctcggag agcatcccta aatacataat tcattacaga gtccatctga ttgcctcgca
    22021 gcatctttcc ggcatatccc caggtctcac ccaacagaat cttatccgga tatttttcct
    22081 tgatctgtat tctcgcctct tcccatacag aagggtctac ttcatctgca acatccagtc
    22141 tccagccatc aattccataa tgatcaatcc agtaaaacat tacttttagt atgaattccc
    22201 tgacctccgg attggaggta tccagttttg gcatccattt atacgctccc acacattcat
    22261 aattatggtg actgggttcc accggataat gattgatatg aaaccatttt acataacggg
    22321 atttttcctg tttttccagt acatcctgaa atggtttaaa atgcactccc gtatggttaa
    22381 atacgccgtc caacacaatc cggattccgt aactgtgaca cgtatccaca agttctctga
    22441 atgtctcttc ggttccaaac tgggaatcta tcttaaagta atctatggtt gcatacttat
    22501 gattaaagtc tgcctcaaat ataggattca ggtagatgca ctctattccc agttccttca
    22561 gataaggaat cttttgaatg attccctgca ggtctccacc catatgatgt tctctgtcag
    22621 gcaaagttcc ccatggttta catccttccg gatcatttcc acgattgcca ttacaaaatc
    22681 tctccggaaa tatctgataa tagatcactc cttttgccca ctccggaaaa gtaatgacat
    22741 ccgtcccatt gacatacaaa tattcaaaga aaccagactt gggaattacc tgctgcaatc
    22801 catttgccgt ataatacatc atatttccac tactgtccgt cagttcaaaa taatattttt
    22861 gataacgtgc aatctgatga aatattattt tcccctgaaa ataatcaaac aacccatctc
    22921 tgtagcagca tttcagttcc tgtttctttt ggttctccgg ttttgttctg tcccagtaaa
    22981 ccaaaataca tttcgatata tccttttttg cacagcgaat cttaaatact aattcatttc
    23041 tctttaccgg atatatgtac tcttccgtat tgaaatgctg tattgcttct tttttcactt
    23101 cttccgtttt tctcctttca ttgtagattc cctctcaata attctcgttg aaagcaatac
    23161 ctcctttctg tgctgtcccg gattctgaat aatggaaaac aacgtattgg cagcttcttc
    23221 tcccagctta tacgtgtcaa tatccactac cgtaagtggc ggatccatat attctgccaa
    23281 tggataatta tcaaatgtga tgattccaat tgatccggga atagctatat ttttcttttt
    23341 taaaaacttc aaagtctcgt aagcaaccac attattggta catataatac tgtcaatatc
    23401 tggatacttg ttgagtgccc tttgtatcgc ttcaccgatc tgattactct ccaccccaca
    23461 ttcaacaatt aaattctcag ccacatcttt cccatttagt cctatcgctg cctgaaatcc
    23521 ctggattctc tttctttcaa acattgttat gcaattttca acaagtaaaa tcgtcttttc
    23581 atatcccatc cggtgtagat aatttacagc atcttctgcc ccctgtttat tatccatatc
    23641 aatccagtga ataccatctc ttacgttatc cggttcgcct aacacaataa acggaaaatg
    23701 attacccagc agtaggtcta tcagttcctc tgttgccgca gaaacaggaa gtataatacc
    23761 atctatcttt ttctctaaaa tcagattttt gacggcacta ttatcctctt tatccgaatc
    23821 gctggtaatc aacaggttaa accctctctg ctgcgttacc gcttctattg ccgaaacact
    23881 tcggataaaa aaagcatttg agaatgcatc actgttaccg gcatcgatta ccagccctat
    23941 cgtaaaagta ctcccattta caaggcttct tgccaggctg ttgggatgat aatccagttc
    24001 tttcatggca tctttaattt tctgcctcgt ttcctctgaa atactggctg ttccattaat
    24061 tactcttgat actgttgacg gatttactcc tacttttttc gcgatatcct taatcgttgc
    24121 tggcatttta tcctcacccg ttttctatga ttgtcacttg tcatgcaaac gtttgcataa
    24181 tcttgtaaaa aaataatgca aacgtttgca ttaactatga tttgatatta tcatgtagct
    24241 attactttgt caatattctt tctcacaaaa tttacagaca acatttattg ttttattata
    24301 ccttgtaaat tccaaaatcg tttgttataa tcaattcaaa gcatatattc tacaattcag
    24361 acagcgaaag ctgttcttcc tatctattat cgtttcaatg gattctatgc ttttacacca
    24421 ctttcacgcg catagtacat tgaaggatag cggatatgga gtaggatcat tcctcttgtt
    24481 ttcatgtact aaccatacga acaggaggtc cctatgaaaa ataaacatga caatcgctct
    24541 ctcccggcaa accggaacta taaggatact attttccgat ggcttttttc cgacaaaaat
    24601 aatctgcttt cactgtataa tgcaatagca ggagcacatt atcagaatcc ggaagctctt
    24661 aacattgtaa cgttggaaaa tgccgtctat atgggcatga aaaacgacct tgcctttgtg
    24721 ttggaaaccg gtctctattt gtatgaacat cagtctactt acaaccccaa tattcccttg
    24781 cgtgatcttt tttatattgc aagcgaatat cagtctctta taaatcagag aaccctgtat
    24841 tcatctacct tacagaccat accgactcca aaatttcttg tattttataa tggtactgac
    24901 gaaaatattt cggatcgttt agagttacgt ttatcagatg cttatgaaaa ctactccgaa
    24961 aatccagatc tggaattaaa agttactatg ttgaacatta attccgatca caacttcgag
    25021 cttttgaaaa actgccatgt cttatgggaa tattctcaat atgtaaccag agttcgtaaa
    25081 tatgctacta tgatgtctct taatgatgca gtaaatttgg ctataacaga atgtattcag
    25141 gagggaattt tgacagagtt cctatcgcat aaccgcgcgg aggtgttgaa ggtgagcatt
    25201 tttgaatatg acaaggaaaa agaagaaaaa ttaattcgca aagctgaatt cgattatgga
    25261 aaagccatgg gactggctga cggcgtaata tcgcttttag aagaaaaagg aagactctct
    25321 gaagaccaaa taaatagcat tcggcgcgaa acccgtccgg aaaaacagac ggaatggttc
    25381 aaacttgcca taaaagttga tacaatagat gaatttttca aaaaagcaaa tataaaaaga
    25441 cagtagcggc tttcgccgta taaaatcaaa aataagagtc atttgtcgta agcaagcttc
    25501 ctcaaatgac tcttattttt gattttgcat ggactcatgt gctataccat cttgaatccc
    25561 tttgccctca gacagatttc cacttcctca ggatctgcgg tatgcaggat catgacgggg
    25621 gtggaagaat cacggttgaa ggacaagtag gtatagtcca cattgacatt gctgtctaag
    25681 agagctgtca gcagggtatt cagtgcaccg ggcttatcct cgatctctac agccaggaca
    25741 tccacgaaac ggcagaggta gcccttctct gtgaggacct cttttgcctt gtcaggatcg
    25801 gagacaacca tacggttgat gccgtactcc gcaccgtcgt tggtaacaga accccagata
    25861 ttgatgcctg cttcatacaa cgcattggtc atagtctgca gggctccttt tttattctca
    25921 gcataaatag ataactgttt taacatttcg gtattacctc ctcattgcaa tctgactgtt
    25981 atgcagtctg tcggaaactt ccgacacttt aatatgataa agcattatta caaaataatc
    26041 aactcggagt tcgcatttga ctaatgctct ttgctaatta ctctatttta taggtgtcgg
    26101 gttcaaaa
//
LOCUS       DAWEJW010000028        25187 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_811_length_25187_cov_60.368216, whole genome shotgun sequence.
ACCESSION   DAWEJW010000028 DAWEJW010000000
VERSION     DAWEJW010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 25187)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25187)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25187
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_811_length_25187_cov_60.368216"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2208..2307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16802..16901
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7456 a         5118 c         6736 g         5677 t
ORIGIN      
        1 tctgttataa cagagcccat gaaatttacg gaccagatct gcctcagatc gtagaagcaa
       61 gactggagaa ggaattgaac tccattatca agaacggatt tgccgtaatg tacatcattg
      121 cgcagaagct ggtatggaag tcggtggagg atggatacct ggtaggctcc cgtggatccg
      181 taggaagctc ctttgtggcg actatggcgg gaatcacgga ggttaacccc ttgagccccc
      241 attattattg cagcaaatgc cattatgtgg actttgacag tgatgaggtt aaggcctatg
      301 ccggtaaggc gggaatcgat atgccggaca aggtatgtcc ggtgtgcgga gaaaagcttc
      361 acaaggacgg atttgatatt cctttcgaga cgttcctggg attcaaggga gacaaggagc
      421 cggatatcga cctgaacttt tccggtgagt accagtccaa ggctcataaa tatactgagg
      481 ttatcttcgg cgcgggacag accttccgtg ccggaaccat tgcaaccctg gcagataaga
      541 cggccttcgg ttacgtgaag aattattttg aagaacgtgg cattcacaag cggaccagtg
      601 agattgaccg tattgcttcc ggatgtaccg gagtacgacg cagtaccggt cagcatcccg
      661 gcggaatcgt cgtactgccc ttaggacagg atatcaactc cttcaccccg gtgcagcatc
      721 ctgccaacga tatgaccacg gataccataa ccacacactt tgattatcat tccatcgacc
      781 acaacctgtt gaaactggat attctgggac acgatgatcc taccatgatc cgtatgcttc
      841 aggacctgac agggagagat cctctgacga ttcccttaga cggacaggat gtcatgagtc
      901 tgttccagaa tacggatgcg ctgggcatta ctccggagca gatcggcgga acaaagctgg
      961 gggcattggg tatcccggag tttggtaccg atttcgccat gcagatggtt atcgatgcca
     1021 agcccaaggc attttccgat ctggtgcgta tttccggcct gtcccacggt accaacgtat
     1081 ggctgggaaa tgcacaggat ctgatcatga gcggcgtggc aacgatttcc accgccatct
     1141 gtacccgtga cgatatcatg ctgtatctga tccagatggg tgtggaatcg gagaagtcct
     1201 tcaagatcat ggaggccgtg cgtaaaggca tggtggcgaa gggtagatgc gccatgtggg
     1261 aagagtggaa agaagacatg ctggcccaca atgtacccca gtggtatatt gaatcctgtc
     1321 agaagatcga gtacatgttc ccgaaggccc atgcggcggc atacgttatg atggcatggc
     1381 gtattgcata ttgtaagatc cattatccgt tagcctatta cggagcgttt ttcagtattc
     1441 gtgccaaggc attttcttat gaaattatgt gtcagggaca gaagcacttg gagagcgtta
     1501 tggtggatta caaaaagcga caggatacgc tttccaataa ggagaaggat gccatgggtg
     1561 atatgcgtat cgtgcaggaa atgtatgcac gaggctttga attcgagcca attgatatct
     1621 ttagagccca gtccagatcc ttccagatcg tggatggcaa attaatgcct tccttaaaca
     1681 gtatcgacgg actgggagag aaggcggcgg atgccatcgt ggaagcggca aaagacggcc
     1741 ccttcctgtc taaagatgat ttccgccaga gaaccaaagt ctccaagacc atcatcgacc
     1801 tgatggattc cctgggatta ttgggagacc tcccggagtc caatcagatc agtatatttg
     1861 acctggtgta acagctcagc cataccatga cgaatgcacg gcttggacgc ttgagtacaa
     1921 agctgcgcat ttgacatggt atgagcggag cgcccaatcc atgaagcaaa agatgagccg
     1981 cgcaagcggc gtcgactgcg cagcagccgc ttttgcgaat gaccgtgggc cgagctgtag
     2041 tccggccagc cgcttttgcg aatggccgtg ggctgagctg gtctaaaaat ttttaaaaaa
     2101 agtgcttgca ttttttcgac aactcgatta tactatgcaa gtactgtttt tgtggctgat
     2161 tggtcaagcg gttaagacgt cgccctctca cggcgaaaac aggggttnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnacg tcgccctctc acggcgaaaa caggggttcg
     2341 attcccctat cagctgctta ttgaaaattg tagaaatttt tcaaaaaata cttgcaattt
     2401 tcaaaagaat gtattatact gaacaaggtt cgtaagtaac ctatggctga ttggtcaagc
     2461 ggttaagacg tcgccctctc acggcgaaaa caggggttcg attcccctat cagctgctta
     2521 ttctttaata gaatagccca accctgaaaa gtgtcggaaa gcatagaaaa ttgctttttg
     2581 gctctttttt tatactgtct ggggctgggc aaatgaaaaa tcttcttgcc attcccattt
     2641 ttttgttgta tagtggttca cgattactca aagcagggga aaatacggca aagaggcggg
     2701 aaccggatgc cgtaggtccg tagacagacg gatagatggg agtgcatatg gcaaaatatt
     2761 tagtaattgt agagtcaccg gccaaggtga agacaatcaa aaagtttttg ggagcaaatt
     2821 atgaagttat ggccagccag gggcatgtga gggacctgcc caagagtcag ctgggatttg
     2881 atccggaaca tgattatgaa ccgaaatata ttaccatccg cggaaaggga gaccttcttg
     2941 cttctttgcg caaagaagta aaaaaggcag ataagattta tctcgcaacc gacccggacc
     3001 gtgaaggaga agctatttcc tggcatctgt attatgcctt gaaactggaa agcaaaaaag
     3061 tatatcgtat taccttcaat gagatcacca agaatgcggt aaaggaatct ctgaaaaacg
     3121 ccagagagat cgatatgaat ctggtggatg cccagcaggc aaggcgtatg ctggaccgta
     3181 tggtgggcta tcggatctcc ccacttctgt gggcaaagat caagagaggc ttaagtgccg
     3241 gccgtgtaca gtctgtggca ctccgtatca tctgtgacag agaggacgag atcaacgcat
     3301 ttattccgga agagtattgg tctctggatg tgaatctgaa agtaaaaggc gagaagaagc
     3361 ctatttgtgc aaaatattac ggtaccaccg aaaaaaaaca gggaattacc agcaaggaac
     3421 aggcagacag cattatggat tccctgaatg gcgcaaaatt cgaagtaaaa gaagtgcgtc
     3481 acggcgaacg tgtgaaaaaa gcgccgcttc cctttacgac ttccacattg cagcaggaag
     3541 ccagcaaggt attaaatttc tctacccaga agactatgcg tctggcacag cagttatatg
     3601 aaggtgtgga tatcaaggga gagggcactg ttggtctgat cacctacctg cgtaccgatt
     3661 ctacccgtgt atccgaagag gcggagacac aggcagcgga atttattgat gttcattacg
     3721 gtgaaaaata caagaccgct gcagcaacgg agaagaagaa cagccagaag attcaggatg
     3781 cccacgaagc tatccgtccc acggatctga accggatgcc tattgctatc aaggagcagc
     3841 ttcccaggga tctgttccgt ctgtatcagc tgatttggaa acgatttgtg gcaagccgca
     3901 tgagtgctgc acagtatgat accacttctg tgaagattca ggcaaaggac cagatttttg
     3961 ctctggcagc ttccagaatg cgtttcgatg gattcatgag tgtctacacc caggaagatg
     4021 ataaggaaga agaaaatgtg ctcggcggta atctggacga gaacagcgtg ctggaattcc
     4081 agagctttga tccgaaacag cattttaccc agccccctgc acattacacc gaagccagtc
     4141 tggtgcgtgc catggaagaa ctgggcatcg gacgtcccag tacctatgcg cctaccatta
     4201 ccaccatcct tgccaggaga tatattgcca aggagaaccg caatctctac gttacggaac
     4261 tgggggaagt ggtcaacaat atgatgaagg aagcattccc ctccatcgtg gatgtgaatt
     4321 tcacggccaa catggaagct ctgttggatg gtgtggaaga aggcacggta aaatggaaga
     4381 ccatcgtgtc caatttctat cctgatctgg atgaagcagt aaaaaaagcg gagaaggacc
     4441 tggcagaggt gaccattgcg gacgaggaat ccgatgaggt ctgtgaactg tgcggcagac
     4501 gaatggtgat caaatacgga ccccacggca gattcctggc atgtcctgga ttcccggaat
     4561 gccgtaatac caagccttat tttgaaaaga tcggtgtagc ctgccccaag tgtggcaagg
     4621 atattgtcat gaaaaagacc aaaaaaggca gaaaatatta tggctgtatc gataatccgg
     4681 agtgtgattt catggtatgg cagaagcctt cgggagagaa gtgtccccgc tgtggcaagc
     4741 tgctgctgga aaaaggcaac aaactggtct gcatggacga acagtgcggc tatgtgaaaa
     4801 ataaaccgac ggaagagaag tagttttata tactgtcaga attttctcat aaattaacgc
     4861 tattttcatt gtaatctgaa attacatgtg ctacaatgtg cataacatct ggtagtttct
     4921 tagtatgtgt agacggaggg aaatagatga gtagcgtaca actgttggac aaaacacgta
     4981 aaatcgggaa attattacac aacaataact ccagcaaagt ggtattcagc gacatctgta
     5041 aggtgatgcg ggaaattctg aattccaatg tactggtgat cagccggaaa ggaaaggtcc
     5101 tcggcgtagg aaaatccgac ggaattgagc cgatcacaga actgctggat gacaatatcg
     5161 gtggatttat tgatccgatg ttaaacgaga gactgctggg cgtattatcc acgaaagaga
     5221 acgtcaacct ggagactctt ggctttgaaa ataccgatac gagaaaattt caggcgatca
     5281 tcactccgat cgagattgcc ggagagcgtt tgggaaccct cttcatttac aaatgcaatg
     5341 aacagtatga tattgatgat attatccttt gcgaatatgg taccacagtg gtgggactgg
     5401 aaatgttgcg ggctgtgaat gaagagagtg cggaggagag ccgtaaggtg gctgtcgtaa
     5461 aatctgcagt cagcactctt tctttctcag agatggaagc catcacccat attttcgacg
     5521 aactgaacgg catggaggga atcctggttg ccagcaagat cgctgacaga gtcggtatca
     5581 ccagatctgt gatcgtcaat gccctgcgga agttcgaaag tgccggtgtg attgaatccc
     5641 gttcctccgg tatgaaagga acctatatta aagttttgaa tgatgccgta ttcgatgaga
     5701 tcgaggaact gaagcgtcag aacggcagaa attaaatgaa aaatgtaaga atttgcaaga
     5761 ataagaaaaa tttaggaaga agagactatt gcgaaaaata gtctcttttc tttatattta
     5821 ttctgagtga gtttatttta ctattgtgtt tttgtcgaaa tactttataa taaaataaaa
     5881 tgggtatatg gtagaaagtg gtttttgggc gctattgtca gaagaccgcg gaatgtgagg
     5941 taatatgata cagacaaacg catttgatta tatcaatgtt ctggaccggg cagcggacgc
     6001 agcctggcag cggaacgaag ccatttccaa taatatcgcc aatgtggata ctccgggcta
     6061 caaaagacag gatgtggcct tcgaatcagt actgcagcag gctcttggca acaacagata
     6121 tgagtccatg gatgataagg tggccaatgt gaatctgagc agactgcggg gcagggctta
     6181 tgttgactat gccaattatt cctatcgcct ggatggcaat aatgtggata ttgaaaatga
     6241 aaatgtaatg ctggcggaga accagctgaa atatcaggga ctgatcagca gtatcaatca
     6301 ggaatttacc aatctaaaga cagtgatgaa atgaggagtt agtgtatgag tatgttttca
     6361 gcatttgatg ttacggcatc gggacttacc gcccagagac tgcgtatgga cgtgatctcc
     6421 gaaaacgttg ccaatgccaa tactacaaga accgaggatg gtacccctta ccgccgtaag
     6481 atcgttacct ttcagcagaa ggggcagcag cagactttca gtcaggtgct gcacaacgtg
     6541 agcagtagct ataccggcag cggcgtgaag gtaagccaga tctttgaaga tactactacg
     6601 gagatgaata tggtatacga tccttcccat cccgatgcgg atgagaacgg ctatgtgaca
     6661 tatccgaatg taaatattgt tcaggaaatg acggatctga tcgatgccag cagatcctat
     6721 gaagccaatt ccacggcatt tggcgccagc aagtccatgg cattaaaagg actggagatc
     6781 ggtcagtaag ctgacgggtc tttattacct ggctccggat agcggcgccg gaatatatgt
     6841 gaggaaggac atggtttatt aaaatggata tttcagcaat tacagctttg aacagtgtaa
     6901 acggtactgc cacagtatct cccacttctc tgaccggaat gctggcggag aaggagaaaa
     6961 aggcagaagg cactatgttc gaaacttttc tgaatacagc aatcgacaat gtgaagatca
     7021 ccaacagtta tctctcggat gccgagaatg agaagatacg atttgcattg ggtgagaccg
     7081 acaacaccca cgaccttact attgctatgg agaaggcttc ggctgcattg cagtataccg
     7141 tagccatccg cgataaggtg atggaagcat acaaggaact gatgcagatc cagatctaaa
     7201 gaagtatttt tattcttgac gtaagaggag agagctgcaa tggcagacaa attaaaagaa
     7261 atacctgcaa aaatactgga atggtggaac aaattcacca ccaggcagaa gagcatcatc
     7321 gttggagcgg cggcggttgt gatatttgca tttgcaatca tcatgtacgt tatgagtaag
     7381 ccccagtatg tacggctgat cacctgtgag acaacatcac aggcttcgga gatcatagat
     7441 actctggaca gtgcgggaat tgcacataaa gaatccaatg acggactgaa gatcgatgta
     7501 gagagcagcc aacagagtgc ggcaaacttg gcactgggtt ccgcaggata tgtaccggat
     7561 gcgttagatt tgaatgatta tctgggcggt agtatgtcta cgaccgccac tgacaaagag
     7621 agactttaca attcctatct ggaaaaatac ataacacaga cgatagaagc acagagcgca
     7681 gtaaaatccg ccaaggtaca tctgagtttt ccgaaagata acggaactct ggcagcacag
     7741 aaggaagagg cgtctgcctt catccaactg gaactggacg gcacctttac ctccgccaat
     7801 gctgcggcac tggcaaaatg tgtggctacc tggctgcgca acgatactac ggcgaatatt
     7861 actattatgg atacagattc caacctgctg tttgcaggtg gagatgatta ttctaccgca
     7921 ggaatcgcta attctatgca ggagctgcag aatcaggcgg aatccatgat cgccaatcag
     7981 gtcaagaagg tactgctggg aacaaaacag tataatgatg cggccgttac cagccacctg
     8041 agcatggatt tctctgatta taaggagacc gtaaaagagt attatgcaaa ctccggcaga
     8101 gatgaaggta tgctttccca tgaggagact tatgagagcg agaataccaa tgacggcggc
     8161 ggtgtaccgg ggacaacttc caacggggag agcggaaata ctacctatgt atctccggac
     8221 agcagcaaca gttcttcctc tacttccgaa accagccggg attatctgcc gaatgagtct
     8281 attaaggata ctgtgacacc ggcaggagga atcaactaca ctaattcctc catatctatt
     8341 gcagcaatta cttataaaga aattcattat gaggatgtaa aacgacaggg gcttctggat
     8401 ggcactacct gggatgaata caaatcccag aacagcgcag ataccaagat gacagttgat
     8461 caggatatgt acagcctggt agccaatgcc accggtatca atgagagtaa tattacgatt
     8521 attgcttacg aaagtcctat tttttacgat aaggaaagca gcccggttac ggcacagaat
     8581 gtgttgagcg tgcttatgct gctgttgatc ctgggactgt tggcatttgt gatattgcac
     8641 agcatgcgta ccagacaggc ggtacagcag gaggaagagg tatctgtgga gaatatgctg
     8701 cagtctaccc ctgaagcaga gctggaagat attgatgtgg aatctaagtc tgagacacgt
     8761 aagatgattg aaaaattcgt agatgagaat ccggaatccg cagcggcatt gttgcgtaac
     8821 tggctgaacg aggactggaa ctaagaaacg gagtaactat ggcaaggaca aaagcagaag
     8881 gtggaatatc gggactgcag aaagctgcaa tcctcctgat ctctctggga ccggagagat
     8941 ccgccggaat ttttaaacac ttaaaagaag aagaaatcga agaactgacc ctggaaatcg
     9001 ccaataccag aagtgtgact cctcaggtca aagaagaagt gatcaacgaa ttttacgaag
     9061 tgtgtctggc acagcagtat attgccgagg gtggtatcaa ctacgcaaag gaactgctgg
     9121 aaaaggcact gggttccgac aaggctatgg atgtcatcgg caagctgact gcatctttac
     9181 aggtaaagcc cttcgagttt gtgcgtaaga cggatgccag ccagctgatc aacttcattc
     9241 aggacgagca tccgcagacc attgctctga ttctgtctta tctgtcaccc agccaggcgg
     9301 cgttgatcat ttccgcattg ccacccgaca gccaggcaga ggtagccaga cgtattgcag
     9361 tcatggatcg taccagtccg gatgtcatca aggaagtgga aaaagtactg gagtccaagc
     9421 tggcaagtct ggtgaatcag gactacacca ttatcggtgg tgtggatgca gtggtggata
     9481 tcctgaatac tgtagaccgt ggtaccgaga aacatatcat ggagactctg gagatcgaag
     9541 agcccgaact ggcagacgag atccgaaaaa agatgttcgt tttcgaggac atattgctcc
     9601 tggacgacaa ggcaattcag cgtgtgctgc gtgacgtgga caacaacgat ctggcagtgg
     9661 cactgaaggg tgccaacgag caggtacaga atgctatctt caacaacctg tccaaacgtc
     9721 tggttgttat gatcaaggaa gatatggaat tcatgggtcc tgttcgtatg aaggatgtag
     9781 aagaagcaca gcagaagatc gtcaatatta tccgtaagct ggaggattcc ggagagatca
     9841 tcatttccag aggtggaggt gacgagatcg ttgtctagta atcttttaaa acggggattt
     9901 acacagcttg tggaagaaga tgcccgggtg atcaatacca acgatctggt agcgaaaagg
     9961 atccgggaac tggctgccaa aatgcagcag gacgaaggaa ccggatttat ctccggactg
    10021 gcggcagata aagtagaggc actggtgacg gacaccgggg atggcgaaga aaatccgcag
    10081 acttccggca atgtgatcaa ggccggagaa gacctgcaga aattaaaaga ggaagccgaa
    10141 gcagaggctc agaagatcat agaggatgcc aaagcaaagg ctgagagcat tctggcggat
    10201 gccagagcac aggcagaagc acagaaagca gacgcactgg agcaggccag aaggcaggga
    10261 cagcaggaag cgaaagcgga ggctgaacgc gcagaggccc agagagcagc ggaatatcag
    10321 aaaaaagctg ccggactgga tacggaatat cagaagagaa tggatgaact ggagccccag
    10381 ttcgtagata ccattacggg tatttacgag catatttttg atgtggatct gcattcttac
    10441 agagaagtgc tctgctatct gatttctgct accatgagaa aaacagagga caaccggagc
    10501 tttctcgtac atgtttccaa agaagattat ccctatgtca gcatgcagaa aaagcagatc
    10561 atggcagggg caactgcacc gaacagtacg gtggagatcg ttgaggacat gaccctgggc
    10621 aaaggtgagt gcatgatcga gaccgaaagc ggcatttttg attgtggact ggggactcaa
    10681 ctgtcggaac tgcgtcagaa actaaaatta ttatcctacg aaggctgata aatggtcatt
    10741 aagaacattg attttgaaaa atataataaa atggcagaaa agagttttta ccgtaagctg
    10801 gggaaagtgg tcaatgtggt aggtctgacc atagagtccg ccggaccgga ttccaaactg
    10861 ggggatctgt gccggatcct tccggatgcg gaaggggcaa ggcccatcat ggcagaagtg
    10921 gtaggcttcc gtgacaagaa gacactgctt atgccctatg attccgtaga tggtatcgga
    10981 ttgggatgtg tggtggaaaa taccggagat cccctgaagg tcacggtaaa cgacaaactg
    11041 ctgggcaaga cggtgaacgg actgggcgtg cctattgacg gcgaaatgat caatggagcc
    11101 gcctatccgg tagaggcagc acctcccgat cctatgtcca gaactattat cgatcaggta
    11161 ctgccgttgg gagtcaaggc ggtagacggc atgatcacgg taggaaaagg tcagagaatt
    11221 ggtatctttg ccggctctgg tgtgggcaaa tccaccctga tgggtatgtt cgcccgcaat
    11281 accaaagcgg atatcaatgt catagcactg atcggcgagc gaggcagaga ggtacgggaa
    11341 tttattgaga gagatctggg tgaagaaggt atgcgcagat ctgttgtggt tgtggcaacc
    11401 tccgaccggc cggctctgga gcgtaacaag gcggccaaga cagctaccgc tatcgcggaa
    11461 tatttcagag atcagggcaa ggatgtcctg ctgatgatgg actccctgac ccgtttttcc
    11521 atggcccagc gggagattgg actggccagc ggagaaccgc cggtgaccag aggatatcct
    11581 cccagtgtgt actcggagat gccgaagctg ttagagagag ccggaagagc cgcagtaggc
    11641 tctattaccg gattgtatac agttctggta gacggcgatg attttaacga gcccattaca
    11701 gataccgccc gaagcattct ggacggtcat attatgttgg acagaaagct gggacataag
    11761 aaccattacc cggctatcga tattttgcag agcatttccc gatgcatgag ccagatcgcc
    11821 accagggaac acaaacaggc agccaacaag ctgaaaaatg tactggcaac ctataatgag
    11881 gcggaggatc tgatcaatat cggtgcatac aaaaagggca gcaacccgaa tattgattat
    11941 gcaatctcca gaatcgatgc tgtgaacggc ttcctatgcc agggtacgga tgaaaaattt
    12001 acctttgaag agaccgtaca gatgctggaa gagctgttcg cagatcaggg gtgagataaa
    12061 tggcgagatt cagatattct ctgcaaaaca tcctgaatat caaagaaaaa atggaaacac
    12121 aggcgaaaca ggaattcgga acggcgcagg cggctctgaa tgtggaaaca gaacatctgg
    12181 agcgcttgaa agaacgcaga agagagtatg aagagcaatc tgcggggctg ttaaaaggaa
    12241 agctggatct gcgggccatt gaagagaaca aggaagcact tttgaaaatg gacagcattg
    12301 tggcaacgca ggcaatccgg gtggaaaaag caaaggaaaa tgtggaagct gccagagaac
    12361 ggatggcaga ggccatgaag gaaagaaaga tgcatgagac actgcgggag aaggcatttg
    12421 aggcttttct gcaggaagaa aaccatgcgg aaagtaaagc aattgatgaa ctgaccagtt
    12481 acacctacgg acagaagaac cgctagaacg gggatgcggg gcgaaaagcc ggcaggtgat
    12541 ggaaaggcat gaggtttagt atggcaagag aaaaaactcc ggaagaaaac gcagcggata
    12601 acgagagaaa acagctggtg gaagagaaaa aaaatttaaa gaaagaacag caggcccagc
    12661 gaaaagaagc aaagcgccgg gcaagggaaa ttgccaaaca ggaagatgaa ctggaggacg
    12721 ggaatgaggg gaacagcctt ttgaccttcg gggccacgat cctgatcgtg gcattgtggc
    12781 ttgctgtgat ctgtgtaatc atcaagctgg atatcggcgg cttcggaagc tcggtagtaa
    12841 caccgatctt aaaggatgtt ccggtactta accggattct tcccggcaac tctgtgactg
    12901 agacaacgaa ttccggtgct tacggcggct ataccagctt acaggatgcg gtggatcaga
    12961 tcaagagtct ggaactacag ctggagcaga tacagaatgc atcttccgcc aaggacgaag
    13021 aactggatca gctgaaagcc gaagttctca ggctgaagga attcgaaaat cagcaggtgg
    13081 aattccaacg gattcagaag gaattctatg acgaggtcgt ttattccgat aagggacccg
    13141 gagcggaaga atataagaaa tattatgagt ccatggatcc ggccactgcg gaatatattt
    13201 acaaacaggt agtgactcag ctgcaggaga gtcaggaagt gcaggattat gcggccgctt
    13261 attcagccat gaagccgaag caggcagcgg caatttttga acagatgacg aacaatctgg
    13321 atctggcagc cagaattctg aaggtcatga gtgcggatga cagaggtgcc atcctgggag
    13381 ctatgaattc agaagtagcg gcaaagatta ctaaaattat ggatccggaa tcttaagaaa
    13441 aagcagaaaa gatccgatac atgatttgta gtataattac aggtcataga ccgtcataag
    13501 gtggtaaaag gggcctggga aaggaggagt tatggcagga acgacaatta aaggtgtcgg
    13561 taacagcggc aatgtgcaga taccggatat ggtcacagca gcgaagcaga atgcgggaac
    13621 cagcttccag gcggtatgga ataagaccca gagcaatgcc cagacaacta atgcagccga
    13681 cggggaccgc acccaggcgg gacggaacga tgtcagacgt ggtgaatctt tgagagccgg
    13741 cggcagacaa cgcgacaatg tagcggatgt ctctgatgga aacaagacga tggaagatga
    13801 cagcctggat gcaaaggatc tggagaaagt tcaggaagtt gtgggaacgg tggtacagaa
    13861 cctgatccaa cagatcacgg atacattttc cgtcagtgaa gaagaactgc aggccaccat
    13921 ggaagatctg ggaatgacag aagtggatct gacagatccc gggaaactga atgaattgct
    13981 gatggcagtc agcggagcac aggattcgtt cgcattactg acggatgaga atctgtacaa
    14041 tgatgtaaaa ggaattatgg atctgcagaa ggaactgaca gggcaggcgc aggaagagtt
    14101 acagctatct cctgaaaaat ggcaggaagc ggtaacacag atcacacagg aaactgtggc
    14161 ggaaccggtg atcaccgtgg aaacagataa aacagagaat gtggccgtta ctacggaaga
    14221 atctactgtg gataccacag aagcaaccgt gacacaggta cctgacagcg gggaaaaagt
    14281 tcccgtacag aacgcacagg agaacaagac cgaagattcc ggcagcagac aggaacacaa
    14341 cgacggtgga cagcatggca atctgttatt acagaatctg aaggaagaca atttcctggc
    14401 acagttacag cagacggcac agacagaaga agcaagaacc gcagatacgc aggacattat
    14461 gcgtcagatc atggattata tgaaagtatc cgtcaaggcg gacagctctg aactggaaat
    14521 gcagcttcat ccccagagcc tgggaactct gcatatacag atggcatcca gaaacggtgt
    14581 ggttacggcc aatttcattg cacagaatga aacagtcaag gcagcactgg aaagtcagat
    14641 ggtacagctg aaggaaaact ttgcagagca gggtgtcaag gtagaagcta ttgaagttac
    14701 cgttcagacc caccagttcg aacagaatct ggagcaggga agaggcaata acagtaatac
    14761 ggcaaatgaa acgggtgcag gtagaaagcg tacccgcagg atcaatctga acgcagcatt
    14821 tgcagaagaa gagcctcaga ccgaagaaga ccggatcgca gcagatatga tgtcggcgaa
    14881 cggcaatacg gtggatttta ccgcataata aataacgaaa ggagaaaaag gaaatggcat
    14941 tggttgcacc cgtggaaaac ggtaagatcg tagagaccgc atcccagact tccgtgagta
    15001 aagctgcaaa gtccagcagc ggaatggaca aggaagcatt tttacagctt ctggtagcac
    15061 agatgaaata tcaggatcct ctggagccta cgtccaatac ggaatacata gcacagtatg
    15121 cacagttctc acaggtagag cagatgcaga atatgtccaa cagtatggat ctgcagagag
    15181 caagttctct ggtaggcaag gaggtctata tcaagaccac cacatcctcc ggcgatacca
    15241 aactggtgca gggaaaagtg gattatgtgt cctacgagaa caacaaagct tacctttata
    15301 tcaacgaaaa gaagtactcc attgatgatc tggattccgt agtggatacg gattatctga
    15361 atgcgtacaa caaggcttac aactttaccg taaagctgaa caagcttcct aatgtaaacg
    15421 gcattgattc cagtgatggc aagaccattg atgatctgga gaaggaatac aacgatatga
    15481 ctgattacga gaaatccttc ctggcaaaag ataccgtgaa cagcttgaat aagtacattg
    15541 aacgtctcaa ggagatccgc aaagctgccg aagaagcaga agaaaagaag gataccgaga
    15601 gcaaggatga gtcagaagaa actgacagca cagagagcgt ataatgcagt aagcagctga
    15661 cgcagagggg cagatggata tggatattca gaacaacagt tttctgtcca ttgaccagtt
    15721 ggcaaatcag tacttaaaca gcagccgcaa gaccgtaaaa gcagcacagg aaaatctggt
    15781 atctttccag gagatcctac agaacaaaag ccaggaacag aatgtttccg gaacgcagga
    15841 tctgaaattc tccaagcatg ccgccgtacg gctggaaaac agaggcattg agctgacaga
    15901 cgcacagctg gaacgcttga atgacggagc aagaaaagcg ggacagaagg aaatcaggga
    15961 ttctctggtg atcgtggatg acttggcctt tattgtgaat gtacccaata agaccgtggt
    16021 gactgccatg aacagcagag aaaccgaaga aaacgtattt acaaatatta acggagccgt
    16081 tattatgtag accggaccag ggatggaggt catgctccct tgaatgacag agagggagca
    16141 cggttccaat acaggaggaa caaagcacta tgatgagatc attgtattct ggcgtagcag
    16201 gacttaagac acaccagacc agaatggacg taatcggtaa taatattgcc aacgtcaaca
    16261 caacagctta caaatccagt tccatgactt tcagtgaact gatgagccag accacacaga
    16321 aggccagtgg tgccaacgct accactggtg tcggcggtac caatgccaag cagatcggtc
    16381 tgggcgttaa ggcaggagct atcaatactg ccattaccac gcagggttcc gcgcagtcta
    16441 ccggtaaccc ctttgatatc atgatcaccg gagacaactt cttcgtagtc agcaacggct
    16501 ccgagaattt cttcaccaga gacggctctt tctatgtaga tggtgccggc aacctggcaa
    16561 tgaccagcac cggttacaac gtcatgggat ggggcgtgga tgagaccaca ggtaacatta
    16621 agcaggatac cgtaaccgca ctgcgtatta tgagtgctgc gaatatgaca tatcctcctg
    16681 aggctaccac acaggcaaat atttccggta ttctggatga gaacgataag gatgtgacca
    16741 gtgccaacgg taagaccgta aacctgaact tcttcgatgc gagaggttac agttatactg
    16801 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncatccagaa ggatggtatg
    16921 atttacgcaa gctatgacaa cggtatgacc aaactgttag gccagattgc taccgcagca
    16981 tttgccaatg cctccggtct ggagaaagag ggagacaacc tttactctgc aaccctgaac
    17041 tccggtgagt tcgatggtat cggcgtagac atcaccgcag gcggtggata catgtccccc
    17101 ggtcagctgg agatgagtaa tgtagacctg tcttccgagt ttactgagat gatcaccact
    17161 cagagaggtt tccaggccaa cagccgtatc attaccgtat ccgatacttt gttggaagaa
    17221 ctgaccaatc tgaagcgtta atatgtaact attcagagcc atgcaaatgc aaaaaaggaa
    17281 gcctcgcaga ggcgattttg caaatggggt tctgaatagt tgtaaaaata aaatgactac
    17341 ataaagggat aggaaaaaac ctgtcccttt tgtatatata aaagatcaag ggaaaaaagt
    17401 actataagaa aaaatgagaa aaatgcacaa aacggataaa gtgtagaaaa tgttcggata
    17461 acagggaagg agaagacagc atgatagaag ttacgaagat caatggaatc aaggtattgg
    17521 tgaatccgga cctgatcgaa ctggtagaag agaccccgga tacggtgttg acactcacca
    17581 caggcaggaa aataattgta aaagaaagta gacaagaaat aaaaaatctt gtaatattat
    17641 acagaaagga tatttttgca agataaaaaa gcagaatgtc aggtgattta agtggatata
    17701 gcatctttag caggaatatt attagcgtta ttcatgttgg tatttggtat cataagctct
    17761 gcgggtgtcg gaggattcgg agaatatctg gatccggctt ctgcaattat tacattcggt
    17821 ggagctttct cctgtaccct catgtccatg agcttacaga attacatagc gggtctgaag
    17881 tcctttactc tgatcttcaa ggcaccggca ctgaatactt ccgagatgat cggcaagatc
    17941 atagagcttt ccaatgtggc acgtaaggaa ggtctgctgt ctctggaaga agcagcaacg
    18001 gatctggagg aacctttcct gaaaaaggga attcttctga tcgtcgatgg taccgatccc
    18061 gaactggtac gtgccatcat ggagacagaa ctggtaagtg tagagggaag acataaagag
    18121 acgatcggtt tctgggatac actggcggcc atgggccctg cctggggtat gatcggtacc
    18181 ctgatcggtc tggtagatat gttgtatcac atggacgatc cgtccacact gggacctgca
    18241 atggcagtgg cgttgattac tacattgtac ggatccttgc tggcaaactg gatctgtacg
    18301 cctgtttcca ataaattaaa agcagataat gctgtggaaa tgcagcagaa ggaagttatg
    18361 atcgaggggc ttttatccat acaggctggt gaaaatcccc gtgtcatcga agaaaaactg
    18421 aagtcattcc ttcctccgaa ggacagaaca agtgcagatg gtgaagagga agcagggggt
    18481 gaagcataat ggcaaaacgt aagccagaag atccacctgc tggatccccg gcatggatga
    18541 ccacattcag tgacctgatg aacttgctgc tgtgcttctt tgttatgttg ttttccatgt
    18601 cttcgataga tgcaaccaag tacagcgaaa tggcagcagc tatgtcgaac agcactttta
    18661 gcattttcaa tgccggatcc acggcaatcg gtgagggtat cctgatcagc aacggcgtca
    18721 gccagttgaa tgatctggat gagtatatca gtaatgccgg aagaaacgca gacagtgaga
    18781 cggattctga taattatgag aaatatgaca gcgcagaagc tttggaagag gcactgaatg
    18841 agaaaaatct ggagagcaac gaagaactgg ctgaaacagt acaggaagct ctggtggagg
    18901 acagccttgc agatcaggtc agtgtgtcat ttacggcaca atatgtgcag ctatctatga
    18961 agggagcgct gttgtttgat tccggaagtg acagcctgaa ggatcaggct aaggcagttc
    19021 tggacaaggt gggagtgatc ctggaacgtt acggaagtaa ttccaccata gaaatcgaag
    19081 gacatacaga taatgtccct attcacagtg cgaggtttgc cgataatgaa gaattgagca
    19141 gcgccagagc actttccgtg ttctattatc tgacagagac tacctctctg gatccatctg
    19201 atctgagaca cgcagggatg ggtgaccgtg taccggtagc tgataactcc acagaagagg
    19261 ggcgcagcaa gaaccggcgt gtagagatca gaatctataa tccatccaca acatattagt
    19321 gtggagaggg aaaggaagaa taagaccaat gaagaaaaat ctgatgacag tattgattct
    19381 ggcactgctg gtggtgaaca ttgtgttgac cagcgtcatg cttgtgagca ttgtgggtac
    19441 gaataagaag accgcacaac tggtagacaa tattaccact gccatgaatc tggaacttac
    19501 cgttccgggg gatgaagaaa caacgaatgt tgcccttacc gatacagagg tatataatat
    19561 tgcagactcc atgacaattc ctctgaagag cgaagcagga gcaaaacagg attacatcat
    19621 gtttgatgta tctttgtcta tcaacaagaa gcataaggat tataagacct atggcagcag
    19681 tgacacgctg gcaagctatg agaacctgat caaggatgct atcacggcta ccgtttccgc
    19741 acatacagag gatgagtgca gagaggatat ggaaggctta aaagaggaga ttctgaagtc
    19801 tgttcaggat ctattccaat ccgacttcat ctataaggtc gcaatcagcg gagtgaaatt
    19861 cggctgatga agcgggaaaa taagatgaca ggaggtgagg gcctgtgagt gatatacttt
    19921 cacaaagtga aattgataat cttctgaaac agttgtctga gggagacctg gatgtagacc
    19981 agatccaggg ggaggacgag aagcaggtca aaaactatga cttcagccgc cctaccaaat
    20041 tctccaaaga acatttacga accctggaga tcatattcga gcattacagc cgactgatct
    20101 ctacgaacct tccggtatat ctgcgcaaga acgtgcaggt atcggtagca agttcggaaa
    20161 cggttacttt cagtgagttc tccaacgcac tttcgaatcc gtcggtgctt ggaattgtta
    20221 attttgcacc actcaacggc aatattatta tagagattgc aaccaatcta tgttatgcca
    20281 tgctggatcg tatgctggga ggcagcggcc agccattgga aaagagcaga gatttttccg
    20341 atatagagtt gacaatctta cagaagcttt tggtcatgtt tacgcagctg atgcgtgaac
    20401 cctggaaaaa cgttgttgag atctcaccgg tgttaagccg gttggagacg aatccccagt
    20461 tcgcacaggt aattgctccc agcgatatga tcgctattgt taccctgaat atgaaaatcg
    20521 gggatgtcga aggaatggta aatatctgtc ttcctttctt tacactggaa gatgtgatgg
    20581 ataaactgaa caccaaatac tggttctcca caatgcagga gaatcacgat gaacactacg
    20641 aggagtacat tgagtccatg atccggagag tagatattcc gatcaaggca gtgctgggaa
    20701 ggagcaccat ttcggtgaat gatttcctga atctgcaggt gggcgactgt atccggctgg
    20761 attccagagt cgataccgac atgaatgtgt atgtgggcaa tattaaaaag ttcacggcac
    20821 tgcccggtac ggatagagat tcgtatgctg ttcagatcac atcggtgatc agagaggagg
    20881 agtaacgatg gatgggatgt tatctcagga tgagataaat gccctgctgc aaggtatgga
    20941 tctgtcggat accgcggatg gcggtgatgc ggcagcagat ccggcaccgg agaacagtac
    21001 ggctgaaaat tataataatg agaatgaagc tgcggagaac gcagcaccgg ttgtcagtga
    21061 cggagaggaa ctgacggatg tagagaagga tgcgattggc gaggtggcca atatcagtat
    21121 gggatcctct gccacaaccc tatattcact ggtaaaccgc aaagtaaata ttacaactcc
    21181 tgtggttacg ctggctacat ggaaaaacct gctggacgcc tatgaaaagc cctgtgtatt
    21241 tattcagatc aaatataccc agggactgga cggtaccaat attctggtat taaaagaaca
    21301 tgatgtaaaa gttatcactg acctgatgat gggcggtgat ggtacgaata cagatggaga
    21361 gctgggagaa ctgcatctga gtgcaatttc cgaggcgatg aaccagatga tgggctccgc
    21421 agcgacttcc ctttccacac tgttgcagac ggtaattgat atcagcccgc ctgagtcttc
    21481 tctttttgat ctgacagagg taaaggatgg caaggaaata tctccgtttt taggaggaac
    21541 ctttgtaaaa atcgcattcc gtatgcagat cgatgatctg gtagacagtt ctatcatgca
    21601 gttgtatccc atcgattttg cccgtaagct ggtagagacc tttatcaata cccagatggg
    21661 aagcctggga gaaagcaacg atcaggcagc aactcagccg gaaagcatac ctgctcccgc
    21721 agcagctccg cagaacaatg caggacagat gaatgccggc gcaaacaata tgactcagat
    21781 gaatatgcag caaccggatc ccatggctat gaatcatatg aatggcatga atcagatggg
    21841 caacatggga atgccacaga tgaacggcat gaaccagatg aatggcatga accagacggg
    21901 tggcatgggt atgaatcaga tgcctggcat ggacatgaat agcatgaacc agatggggat
    21961 gaatggtatg cctcagatgg gaatgcagca gatggggaac atgggaatgc aacagatgaa
    22021 tggcatgggc atgatgaacc agatgggcaa tatgggaatg ccgcagcaga atgtcaatgt
    22081 gcagcctgca cagttccaga gcttctcaaa tgacagcatg ggactttccg gacaggaaaa
    22141 tattggtctg atcaaagatg tccctttgga agttaccgta gagcttggca gaacgacgaa
    22201 atccatttcc gagattctgg agttctcacc gggtacgatc attgaactgg atcgtattgc
    22261 aggagaaccg atcgatgttc tggtaaacgg aaaatttgta gccaagggtg aagttgttgt
    22321 aattgaagaa agctttggcg tccgtattac ggaaattatt aaataaaaca gagaatagac
    22381 caaacaaatg gaggaaaatt aaatggcaaa gaatattttg atctgcgatg atgcagcgtt
    22441 catgagaatg atgattaagg acattctgac caagaacggt tacaatgttg caggagaagc
    22501 agagaacgga atgaaggcag tggagaagtt caaggaagtg aatcccgatc tggttctgat
    22561 ggacatcacc atgcctgaga tggacggcat ccaggcactg aaggagatca agaagttaga
    22621 cggtggcgct aaggttatca tgtgttctgc aatgggccag caggcaatgg ttatcgagtc
    22681 tatccaggcc ggtgccaaag atttcattgt aaaacctttt caggcagaac gtgttattga
    22741 agctgtgaaa aaggtagtgg gttaatgatc atacttaccg gaaaagcaga cagttatgcc
    22801 cagttcatta cagtattgtt catctttgtg gcagtactgg cggtaaccgc attggtaacg
    22861 aaatggatcg caggatacca gaaacaacag ggagcggcag gcaatattga agtgagagag
    22921 accgttcgga tcgcaaataa caaatacatt cagctggtcc ggatcggtga gtcttatttt
    22981 gccattgcgg tctgcaagga taccgtaacg gccctgggac agattcccgc agaacaactg
    23041 aaaggtaaga ctacattgga aagtacttcc gggttccggg aattattgga acgtgcggcg
    23101 aagaaagaga atgaagagaa agatcattga tgcaagtata aaattaaaga cgattatcta
    23161 tatggtatgc ctgctggtca cggtaggcat attgtgtatt tgtacaccgg taaaggtgca
    23221 ggcatcggag cgtcatctga cgggagatac ggaagtttcc acggtgatca atccggcggg
    23281 aacagccacg accccggaag aggtaggaca gctgaatacg gcgaatactg tcagcattac
    23341 ctacaataac ggtaacggac agatcaacgg tgcgcttcgg attctgatca ctctgacgct
    23401 gatcgcactg gcaccgacca ttattatcat gatgacctct tttacgagga tcatcatcgt
    23461 gctgcatttt accaggtctg cactgaatac ccagacggca ccgccgaacc agatcctgat
    23521 cggactggca ctgatcctga catttttcat tatggagcct acgatcacga ggatcaatga
    23581 ggaagcaatc cagccttttg aggagggcac gatcaatcag gatgaggctc tggaaaaagg
    23641 catggctccc ttacgggaat tcatgtatcc ccagacccag gtgaaggatg tggagctgtt
    23701 catggatatt gcgggtcagg agtgggacgg aaccctggag gatattccga attccgtgct
    23761 gattcccagc tttatgatca gcgaactgcg aatggcgttc tggatcggct ttatgatcta
    23821 tattcctttt attgtaattg atatggtagt ggcatccact ctgatgagta tgggtatgat
    23881 gatgctaccg ccgacgacga tctccatgcc cttcaagatc ctgctgttcg tactggcaga
    23941 cggttgggca ctgatcatag ggcaactggt acagacattt tattagattc gtaagatgag
    24001 gttttgctat gacgatagat gcagtatcgg aaatgacgaa taatgcgtta tatgtgatca
    24061 tcaaggtatc cctgccggtg ttgctggtat ccctggttgt tggtctggtc atcagtattt
    24121 tccagacggt gacttccatt caggagcaga cactgacctt tgtaccgaag atcgttgcca
    24181 tctttttatg tctggtggtt atcggacact ggatgatgaa cacgatggtg gagtacacgg
    24241 tgcaattgtg gtcaagtttc agccagtatg tacataggtg acgggaagaa atatggtaga
    24301 tctttctttt tcaatatatg atcttgaata ttttctgctg atattcgtaa gagtatcctg
    24361 ctttgtatat atagcgcctt atttcgggat gaatgacacc ccggccagaa tccggatcgg
    24421 catcagcttt tttacggcgt ggctgcttta tgagacgctg acgccggcgg atgcggtagt
    24481 tgttgacact gtgatggaat atgcggtgat cgtcatgaaa gaggcaattg ccgggctgct
    24541 gatcggtttc ggggccaata tctgtatggc agtcgtgaat tttgccggtt ccattgctga
    24601 catggagaca ggcctgtcca tggctacgtt actggatccg gctacgaaag agaccaccag
    24661 cattaccggt gtcttgtatc agtattcttt tatgctgatg ctgatcgctt ccggaatgta
    24721 caggtatctt ttcggagcac tggcagacag ctttacactg atcccggtga acggggtggt
    24781 gtttcacgcg gacagtctgt tacaatccat gacagaattt atgagtgatt atatcctgat
    24841 cggctttcgt attgtattgc cgattttttg tgtgatattg cttctgaatg caatcctggg
    24901 agtgctggcc aaggtgtctc cccagctgaa tatgtttgca gtaggtattc agctgaaggt
    24961 tctggcggga ctgacggcaa tgttccttac caccgggatg cttccggggg ctgccgactt
    25021 tgtctatcag gaaatgaaaa agatggtagt atcttttatc ggaggtatga tgtgatcctg
    25081 gaatatgatc tgcaattctt cgctgcagaa gggatgggcg gagagaagac agagcctgct
    25141 accgagaaaa agctgtctga tgccagaaaa gaaggtcagg ttgccaa
//
LOCUS       DAWEJW010000029        24929 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_821_length_24929_cov_61.931857, whole genome shotgun sequence.
ACCESSION   DAWEJW010000029 DAWEJW010000000
VERSION     DAWEJW010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 24929)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24929)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24929
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_821_length_24929_cov_61.931857"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1070..1169
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4861..4960
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6884 a         5313 c         6605 g         5927 t
ORIGIN      
        1 gattatgtga acgccggaat cggagctacc gattcccagt tcggcgcggc gagagtacag
       61 gaagacctgc ttcagcggct accggatctg gtatttgtag aattttccgt gaacgatcac
      121 agtacgccgc atttctgtga gacctacgaa ggtctggtga ggcagattta tggcagtgcg
      181 agcgcaccgg caatggtgct gatccataat gtctattatg ataccggtaa aagtgcggca
      241 tattatcatg cgcaggtagg acgacattat gatctgccct gcatcagtat gcagaacagt
      301 atctatcccg ctgtggcagc cggaagactg ccggcagaga agattacggc agatttcctt
      361 catcccaatg atcttggtca tgaattcatg gcatctgtga tcacgaattt cctggaaaag
      421 gtaatccatg ataaaacgca agagccgcag gaaattttcc cggccccgct gacagagaat
      481 gcctatgagc attgcgtgag actacaatat tttaattcca caccggagaa gagtggcttt
      541 gaagaagata tgacaccgca gagaggcatc acggatattt tccgtaacgg atggagcgcc
      601 ggagaaaaag gagcttatct ggagttcact ttccggggaa ccggtgccgc cgtgcaatac
      661 cgccgagtga aggacggacc tgcacccatt gccacagcag tggtggatga gaaccctgag
      721 acggcgtgcg tgttggacgg aacctttgat gagacctggg gagataagat ggaactggca
      781 acgattgcgg agcatctgcc ctatggggaa caccgggtga gaatcgagat cacggagaca
      841 cacgagaacg ataaggctgg attttatctg gtgtcattga ttgtatccgg ggtaagaaca
      901 gaaacagaag cggaataggc atagtacgga agtcaggtag acagatcgga agaaatgtgt
      961 tacatatcag atagaatatt tttccagaaa cggacgttgc aaagctaagt agcggaataa
     1021 cgggaaagat gccgtgcaat acatagtaag tatcatggga aaggatagan nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tggagacctg gcaggataag ccctttgttc
     1201 tcatgagcgt ggtagagggc gaaggcaccc tgaacggtac tcctttaaag aagggcgatc
     1261 acctgatcct gcccgacggc tacggtaagg tagagttaca gggcaggatg cagatcatcg
     1321 catctacgat ctgagttcac gacagatcaa actccggtca cttgatttat ttcccaagta
     1381 caacggatag cttgatttct ttttctgttt cgctcaggtc gaaatcaccg ccgtcggaga
     1441 cggtcacgcg cagttcatag agcacgccgt ctgtcagctc cgtcacattt gctccggtct
     1501 cgtctgtgag cgaccacagt tcagagccaa ccttttccag cttgccgtct gcaccatagg
     1561 cgtagagcgt cagatcggag gcactgccgg acaggttgtt catgcggaag gctaccatgg
     1621 tgttgggatg cacgttgcag atggtcgcgg tgttgtgcca gcttgcaacc gtaccttcac
     1681 cgccatcctg ctcaaactga tcctgcgaaa gaacatttct tgccgcctga tcaagatcaa
     1741 cctcacggta cggaagctcg ggcaggtaca caaagcgatc ctgcaaacgc agcagttcca
     1801 gatagcctgt cgggcagtag agcgtatttc cgttgctgcc ggtgtacatg ccgatagcca
     1861 tattattgta gccgtatttg ttggacacat ccatcgtgaa gacggtttcg cccgttgcaa
     1921 agtccaaaat aatgtactgc cacatgccgg tatccaaatc ctgtacataa ccgtagatat
     1981 agcctgttgc agtggagagc ttcatgatcg ccgtgtcgcg caaatcgttc cggctccaga
     2041 cgctcttcat ttcatagcca tccgaggttt ttaaggtgtc cacccgctca acgcccgggg
     2101 cgatcataac attacccttc atcagccagt tctggtcata aatgttggcg tagctctgaa
     2161 tcgaagagtc attgtcaggg tcggcaagac ctgcgttacc agcgccgaac cagttgcaga
     2221 cgatcgtgct gacggtgcct tcaccgtcgt cataaacgat tgcagagttt tcaaccgcca
     2281 cctgataccc atcgggcaga tcatccaaaa caggcatgct ggctacgacc tctccggtct
     2341 tcatgtcgag cgcaagaagc ttaacggggt ctgcgttgtc ggtgaacagt acaaggtttg
     2401 gtgtcagtgt gggcgagcag ccgccacccc aggcaaggcc gccgccggta gtcttatcgc
     2461 catcatgact gaccttcgca ccgacactct catatggcgt gcaccacaca acgttcacac
     2521 cttcttccgc gcgcaacaga tagcagttct ggttcgtcag aatgaccgca ccgtctttgg
     2581 aggcagcaat gccgttctct gcgccctcgc caaggggcag cccgtaaaca aagacagcct
     2641 ttgaaagatc ggtttgttcg ccattcagaa tcgattctat cgctgtgtgg gcaatgtagc
     2701 cgagaacgcc ctgctgctgt cgttcgggat agattcggaa gccgccggtg gcgaaccaaa
     2761 ggtttccgtc gtagtcaaag accacggaca gaagattctg tgtcagctct ttgccgagcg
     2821 cggtctcagc agctgccttg atatcgatat ccagaacctt ttcaaattcc ggaatgacgc
     2881 tgccatcttc ctctgtcgta cgcagtatca gtacatggtt gttgctcgtc gggcagacga
     2941 cacggttttt agagtcaagg aatgtgtagg aactctggat gacgtaaccg ccgccatcat
     3001 gctgcatcgg tgaaaaatag ccgagcgtct tcgtctctgt tgcattcaga tcacggatgg
     3061 cgatgccgcc gagcagtggc acgaccgcgt gaccgtagtt gtcaaagtaa atcgcgggtg
     3121 aggcgttcgg gttcgttgtc tcaaaggaaa cattgatttc cggatagatg ccgagcggca
     3181 gaatttcatc cgttgagtcg gtgttgtagc cgtcgtgatg gatgtttgca tcactttttg
     3241 ccatataagg gttttcatcc acgtgtttgg gttgtaaagc cttcacgggc agtgcaccgg
     3301 ataacggtgc agaagatgtt gtttcgtcct ctgccggtgt gggcgttcca ccacaggccg
     3361 ccatagacag gcacagtgtt cccgccagaa gaaagctaaa tacagaataa atcttttttc
     3421 tctttttcat caataatcct cctcattgtt atgtttgttg tgatccgccc ggaataaggc
     3481 agacgttttt ttcccatctg accatgtaga aagtccctgc tgaaacgctt cttctgttat
     3541 tggctctaac agaaaatagt ctacacgcag ccgaaatgca tgcagggaaa aatccagatc
     3601 gctgcaccag atgatgcgtg tttcgggaaa aagcgttcgc agatgctcca ccgcattcaa
     3661 cccttctact cccggaagtg cgatcacagt gcaggttggc gcatttacac gcgccacatc
     3721 aaagaacaac tcctgcccac ggtaacagtc cattttggga aacagaccgc gttcaaagca
     3781 gaaccgtgaa agctgctcta tataaaaggc ttcctgtgtt tcgctacttg tcagcacggc
     3841 aatgcggtac acagcactcc ctcccttctg ccgtcattga tgctttctgc ttgtcagtat
     3901 acggatatgc aaacgctttt caagccgttt ggcacgaaac tctcaattcc gtcacgaaat
     3961 ctgagttttt cattgtgccg gtgttggaca ggtatgatat aatttagttg gaatttttta
     4021 caaaggagtc agtccgatat gagaatagca atagtggatg atatcgagac cgaacgtgcg
     4081 ctgttgaaaa tgcggcttga gcgacagctg agcttgtacg gtgtagagac ggagatctta
     4141 gaatttgaca gcagcgagag ctttctcgca gcagaaaaag aacggcactt tactgtggca
     4201 tttctggaca tttacatgaa aggattaagc ggcatggatg ccgcaaagga acttcggaag
     4261 acggatgcag actgctttct gatcttcacc acaacatcta ccgaccatgc actggaggga
     4321 tttcaggtgc gggcatttca ctatctcgtg aagccttttt ccgagggaga acttgccggt
     4381 ctgcttgctg aactgctcgc taaacttccg cgtccggagc cgattctgac agtgaaagtc
     4441 agtggcagtg acgttcgcct tcgctatagg gacattattt ttgccgagca ttttgctcat
     4501 atgatcaaca ttcgtacgac tgccggtaaa acgcttgcca cgcggcaaag ctttaaggca
     4561 tttaccgagc cgctgaaaaa agatccacgc ttttttgtct gcggccgcgg tacgattgtc
     4621 aatttagaac atgccgctga ttttcaggcc gccgctttct gcatgaccga cggtagccgc
     4681 gtatatgtca accaggaact tttgaagcct gccaggcagg catttatgga atttctgttg
     4741 caagggggtg tatgagatga aagatattct gcgtccgatc ctggagttgt ccgttgtact
     4801 ccccggtctt ttacttgcat atttccctgt aaaatcctat ctgaagcagt cccccggaaa
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taatggccac cagtctcaac
     4981 cgcaacgcga gactacagca ggaaaaccag cttcttttcc tgcagcagca acgctatgag
     5041 aatctcaaag ctgccatcga agaagtgcgg caggcgcggc atgatatgcg ccatcaactg
     5101 aatcaaatta ccgcactggc tgagaccggc gatctggagg gattgaaaag ctaccttgaa
     5161 aaaaccattt cccgcatccc gaatctcggt atacacttct gcgaaaaccg cgcagcagat
     5221 agcgtcatcg gctattattg tgccctggca aagcgcgaag acattccctt ttgtgcaaag
     5281 ctcgatctgc cgcagactct tccaatcgat gagattaaca tgtgtctggt cttgtccaac
     5341 ttgttggaaa atgcgctcga agccagtatc cgcactgcac cagacagacg gcagattaaa
     5401 ataactgcat acctgcatgc cgggcggctg cttctgatcg aagttgagaa tgcctatgac
     5461 ggcgaaatca gggagaagga cggtgttttt caatcctcca aacgaaaggg aaacggcgtc
     5521 ggcatccagt caatccaaca tattgcagaa aaaagcggcg gtgcaagtac gtttgcctat
     5581 cagaatggtg ctttctgtgc taaagttatg ctctgcggat aaatgcacat ttctgccgat
     5641 attctgtcgg tgtcattccc acaaatttct taaactggcg catgaagtgt aattcattgt
     5701 cgtagccgca gaaatccgaa aggctgcgga tgctcatatc acttccggtc agataatatt
     5761 ttgccagttc cagacgggca cttatgatgt cctgttggca ggaacaggtg aagaactgtt
     5821 tataaagatg ctggaagtaa gagagactta gggccagaga atccgccaga gccgggatgc
     5881 tccagttatt cgccggatta ttgtagatct gtgtccggag tccggagaag gcagaataat
     5941 acttctgcac gctgggatcg ttggaatttt cttccagctc ttcctgtaaa gcatacagga
     6001 aaatatgcag aaaggcatca atagactgct ctctgtagcg ggagtcaccg taaaaaatat
     6061 cggaaagcat gcggacatat tccgacagtc tgtgaaaatt ataaggatgc agaatctgac
     6121 agaacggagg caataattca agaaaaatat cccgttcctg ggcatccgga agaaaatgga
     6181 tccagtcatc gttatacccg atggcatcac agccatagtg gatataagta tccggcggga
     6241 aacaaatgag ggagttggga gcgaccgtgt gtttttcccc gtccagataa atccaggatt
     6301 cccgcttgat aagcagaata aggtagtctg gaactccgcg cttatgctcg atattacatg
     6361 gttttgggtg gtgggaatca tgcccgcagc tgttgacgta tatcatgatg atatctcctg
     6421 taaaaagcag aatatgtcag ttattagcat tgtaagatat tgcatctgcc gtgtcaattg
     6481 attaccatga aattaagaaa acaaaggagg tatccgacat ggtttgggag atccgggagt
     6541 acggagcagt ggatgacggc agaaccgtca atacggcggt gatccagaat acaatagaca
     6601 gatgccatca ggcaggcggc ggtaccgtac tgatcgaagg cggagtgtat ctgtgcggaa
     6661 cgatttttct gcggagcaat gttacgctgg aaatcgcaca gggaacggtg ttaaaggcaa
     6721 accccgacat cagtgattat gcggagaata cccatcataa cagatatcgc aatgaggaag
     6781 cactggacag atgctttctc tacggagaag atctggaaaa tatcgggatc tgcggaaaag
     6841 gacgtatcga aggcagttcg gaggcatttc ccaacaaagg caatatttac cgcccgatgc
     6901 tgatccgttt tttgcggtgc cggcagatac atatcgagga catccgatta tgcgacgcgg
     6961 cggcatggac tacggcgttc ttagattccg agtacatctg gatcagcaaa gtatatattc
     7021 ataacgagaa acgctataac ggcgatgggc tggattttga cggctgcgcc catgtattta
     7081 tagataattg tagtattacc ggaacggacg acaatctgtg cctgcaatcc agctcgaaag
     7141 aatatcccgt gaaggatatc catatcagca actgtgaatt cacatcccta tgtgcgggga
     7201 tccggatcgg cctgaaatcc atcggggata tctacaacgt agttatcagt aactgcacta
     7261 tggacagagt ctggcgggaa ggcatcaaga tcgaatgcac ggaaggcggc agcatctcgg
     7321 atatcatgat ccagaatgtg acgatgcggg atgtaacaag acctgttttt atgatcctga
     7381 ataaccggtt caagacagac gattatggaa gctccgtaga actggaacag atgccggaga
     7441 tcggcagaat ggaaaatatt acgatcaccc atctgacggc ggtggattcc gaagaaatga
     7501 agaacaccca ttatcgtttt ggcaatgata ttatgggaga accgaagttt aacggcatcc
     7561 gcatagatgc ggaggaacat catccgatcc gggacgtgac tttacagaat atctgctacc
     7621 ggagtatcgg tggggtaaag aaggcagaca ttcccgcgga atacccgccg gtgctggatc
     7681 aactgcggca tccgggagag gtgacctcgg agaattatta tcccgactgg agccggacgg
     7741 catatctgga tatccggaat gtggaggaac tgtatctgga caatatctgc tttgaatgcg
     7801 tggaaccgga tgagagggaa ccgtacctga tcgagaacag cagcatcctc agacaggata
     7861 tccgggtgaa agcatagaaa acgcgcaaac cgtgaacata gaacaaataa caggaaaaag
     7921 aacaggagaa aaacatggaa aattatcagg catgggcaga agaggtttta gaaaaagtaa
     7981 aagagaaaat ggtatgggtc agtgaaaaaa acaaggatat catcccttac accaccgatg
     8041 cgaacggtaa ttatgatgac cgttcctcag aagagaaaga atggaatcac gatgacggtt
     8101 taaactggtg gaccaacgga ttctggggcg gcattatgtg gctgctgtat caggataccc
     8161 aagaggaaaa atatgcgcag attgcacggg tttccgaaca gaaaatggag aaatgttttg
     8221 aaagctattt cggacttcat catgatgtgg gattcatgtt cctgcctacg gcagttgcag
     8281 attatcgctt gaccggccat gaggattcca aacggatcgc catgcatgca gcaaacctct
     8341 tggcaggcag attcaatccg gtgggaaagt tcatccgggc atggaatcag gtgccgggca
     8401 gcgataccag aggctgggcg atcattgact gcatgttcaa catctgtctg ctctactggg
     8461 cttccgagga aagcgcagat cccagattcc gtcagatcgc catgatgcat gcagataccg
     8521 taatggagaa tttcatccgt ccggatggtt cggtatgcca tatcgtggaa ttcgatccgg
     8581 agaccggtgt catggtaaaa agccacggtg gacagggcta cgcggaaggc tcttcctgga
     8641 ccagaggaca gggctgggca ctgtacgggt ttaccaacag ctatacccat accggcaaac
     8701 aggaatatct ggataccgcc aaaaaagtag cacactattg catcgccaac atcccggaga
     8761 atggtattat tcccgtagac ttccgtcagc ccacgcagcc ggcctgggag gattcctgcg
     8821 gtgcatgtat cattgccggt ggtctcttag aggtagcgaa gcatgtaccg gagctggaga
     8881 aagagatgta catccgtgca gcggttaaga tcctgaaggc cattgcggag accagagcag
     8941 atttcacaaa ggactgcgat gccatcgtac agaactgtac cggagcctat cacgacagca
     9001 gacatcattt taccatggta tatgcggact attattttat cgaggcattg tataaactta
     9061 ccggaaccgg tatcctgttg tggtaagctg ggaatagtag agcaggaaat ctgcggagag
     9121 gaaggacagg caaatgaaat taggtggaat agaagccggc ggcaccaaga tggtatgcgc
     9181 cataggggac gagcagggca atatttttga cagagcggta tttcccacgg agaccccgga
     9241 gattaccgta ccgaagatga tagaatattt tcaggataag ggaattgagg cgctgggcat
     9301 cggctgcttc ggcccggtgg atctgaacag gaaatcggct acctacggat atatcaccag
     9361 tacgccgaag ctggcatgga gattttacga ttttgcggga gccttccggg aggcattgca
     9421 gattccggta ggttttgata cggatgtcaa cggggccgtg ctgggagagg tacgcttcgg
     9481 tgcagcgaag ggctgtgaaa atgccctcta catcactatc ggtaccggtg tgggcgtagg
     9541 cgcttacatt aacggaaaac tgctgcacgg actgatgcat ccggaaggag gacatatttt
     9601 cctgcaaaag catccggagg atacctacgc aggctgttgt ccctaccacg gtgcctgtct
     9661 ggaaggtctg gcctccggcc tggcaattca gggcagatac ggcaaaaaag gagcggagct
     9721 ttccggaaga gacgatgtct gggaactgga aagctattac atcggtcagg ccgttgccga
     9781 ttacatgatg acctattccc cggagaaaat catcctctgg ggcggtgtta tgcatcagga
     9841 gaaggtcttt gacatggtca gaaaaaatgc gaaggagttc ctaaacggtt atctgccgga
     9901 ggtaagcctg ccgaaggatc tgtcacagta tattgtggct ccggcactgg gagagaatcc
     9961 gggcatcatc ggggcgttgt gcctggcaca ggaggaaatg gtatgcagca gatagcaaaa
    10021 ggaatctgga agatcgtatt gggagaaccg gaaacgttca caccggagca tttcaggcag
    10081 tttccggtca gaacagaagc catagaacag attccggtga gccgggaatg cagagtcagc
    10141 gaggagaaaa tccactggag aaagacaaaa cggggaatca cggtcaccct tcccatggag
    10201 acacaggaag atatctatgg cttcggtctg cagcttcagg gattcaatca ggcgggcagg
    10261 agacgttata tcaaggtaaa ctccgatccg gtggcgaata ccggggaagg ccatgcgccg
    10321 gtaccttttt acatttcttc cgcaggatac ggattatttg tgaatacctt ccgttacacc
    10381 acctttttga tgggaacgaa cagcgaacgt gggcagtctg ccgggatgac ggcagagaat
    10441 gaagcccata aagagttttc cgaggctgcc atttatgcgt taaagagagc gaaagaagaa
    10501 cgaaaagtga tcatagatat cccggcggca gggggtattg aactgtatct gttcgcggga
    10561 gactttatgg aaatcgtcca aagatacaat ctgttctccg gcggtggctg tgtgcctccc
    10621 atgtggggac ttggtatgtg gtaccgcatc tatggcggcg gagatgaaaa ggctctgaaa
    10681 gaactggcag agcagttccg caaggaagaa attccgatgg atgttcttgg actggaaccg
    10741 ggatggcatt cccatagcta ttcctgcact tataaatggt caaacctgtt ccctcatccg
    10801 caggaaatga tcgatgatat gacagcacag ggctataaac tgaatctgtg ggagcatgtc
    10861 tatgtatatc ccaaggctcc gttctatgag aaactggtgc cttacagcgg tgattatgag
    10921 gtgtggaacg gactggtgcc ggatttcgcc acgaaagagg catgtcagct attcgaagat
    10981 taccacagag acacgttcgt aaagcagggc attgcgggat ttaagctgga tgaatgtgac
    11041 aactccgatt acaatccttc caactgggcg ttcccggatt ccacggaatt cccttccggc
    11101 atggacggtg agcagatgca catgaccatt ggaaccctgt atcagaacct gatctaccgg
    11161 atctatcaac aggagaaccg cagaacctat tcccaggtgc gttcctccgg agccttagcg
    11221 gcatcgttac ccttcgtact gtattcggat ctgtatgatc ataagcagtt tatccgcggt
    11281 gtggtaaatg cgggattttc gggacttttg tggtgcccgg aggtgagaga ctgtgccaat
    11341 ggggacgatc tgctcagaag attgcagacg gtagtatttt ccggacatgc actgatcaac
    11401 agctggcgga ttcccagtcc cccctggaaa cagaccgaca ttgagaaaaa tcttgccggg
    11461 gagcccatgg atacggcaga gtattatacg gcggaatgta gaaagttgtt tgaactgcgc
    11521 atgcgcctgc tgccctattt atacagtgct tttgtggagt acagccatag tggcaagcct
    11581 cccatccggg cactggtgct ggattatccg gaggataggg aagcgcgtat ggtggatgat
    11641 gagtatctgt tcggcgagag catgctggtc tgccccctga cctacgagga tggcacttcc
    11701 agaagcgtgt atctgccgga gggcagctgg tatgatttct ttaccggtga aaggacagaa
    11761 ggtggaagaa gagtggaagt acatgcggag tacgacaaga ttcccgtatt tgtaaaagaa
    11821 ggcgccatca ttccgctggc gaaaccggtg acccatgtgg agaaggatac ggtattcgac
    11881 atcacagtaa agacattcgg ggaagcggat ggcagctttg tactgtatga ggatgatttc
    11941 gagacttttg cttatgagga agggcagaac cgtatcgtga tcgaaaagca tccgggagag
    12001 gatgtgacga tccactccga aaacggaacg atgacaaaat atcggttggt gaggtaaaac
    12061 aatcatggaa aatctgtgtc tgaagatcat gaataaaaat atggaggcgc tggcagtggc
    12121 cagaggggaa aatgaggtga atctggtcta caccggagag tatcaggagg gagatattat
    12181 catcctggaa gtgtccgaag gcagtcatta tgcatggctg caatgggacg atgccatcgg
    12241 aaagtccatg gtatatctga cgggaaatgt aacctacagg atccccttcg gagaccgccg
    12301 gatcaacctg tctccgaaag catttgctgg gaagaagcat ctgctgtcag taaagctggc
    12361 caaagaattc gagatacagt attaccggaa tctggcagta aatgtatgtg atcagcatga
    12421 gattgaaaac atatatcccc atgccaccgc caatgtggag accagaggcg aggccgtgtt
    12481 cgcggcgcag aatgccattg acgggtatac cgtaagcagc tgccatgggg aatggcccta
    12541 ccagtcctgg ggcatcaata gacaggagga tgccagaatc cggatcgatt tcggaagaga
    12601 agtgctgacg gatacgattg tggtttacct gagaacagat ttccctcatg ataattggtg
    12661 gcagcaggta tcctttgaat tctccgacgg cagccggatg gatgccgcca tgcagagaaa
    12721 agaaggacct caggagatcg ccatcgggca gaagaagatc agctggctgg aaatgtgcga
    12781 tatgaagaaa tcttcggagc cgtctccttt tccggcactg acccagatcg aagtctacgg
    12841 aaccgaagtt cagggcagga aggagtagcc tatgtttgaa aatgggaaaa tgtgggcgga
    12901 tgatgttgga aagccgattc aggcccatgg cggttgcatc attcagcatg acggtatgtg
    12961 gtactggtac ggagaacaca agggggccaa gaactgcccg ggtaccaccc gtgtggatgt
    13021 catcggtatc agctgctatt cgtccaaaga catgaacagc tggcgctatg agggactggc
    13081 actggatgtg aaggacagcc cgaaaggcag tctcatacag ccggaaaacg tctgcgagcg
    13141 tccgaaggtc ttatataata aaaaaaccgg aaaatttgtc atgtggatgc atctggacac
    13201 tccgaattat acttatgcgg gagtagcggt agccgtagca gaccgtccaa tcggtccgtt
    13261 taccgtgatc cgggaaatgc agccgaaccg gacggacagc agggacatga cactttttaa
    13321 ggatgtggac ggtacagcgt ggctggtgca cagctccgac tggaataaga cgctgcgcct
    13381 gtccaggctg acggaagatt ttacggatgt agatggattt tatgcatccg ctctgacgga
    13441 tcagaccaga gaggctccgg cactcatgta ccgggatgga aaatattata tgatcacctc
    13501 cggatgtacc ggatggcagc caaacagtgc gttatatgcc gagtgcgatc atctctgcgg
    13561 tcagtggaag ctgatcgata atccctgtga gggcgacaat tacagacgca cctttgaagg
    13621 acagagtact tatatttttg aaaaggacgg gaagacatat ctgatgctgg atcactggaa
    13681 accaaagaat cttaagaatt ccggttacag catccttcct gtgacagtag agaacgggat
    13741 attgaccgta aagtggcagg caactttccc ggaacaggca tcatagtcgg gggcttttgc
    13801 ccccgatatc ataatacagt aagcggaggg ttattatgat tcacaatccg atcttaaaag
    13861 gctttcatcc cgatccgtcc atcgtgcgtg tcggggaaga ttattatatc gcaacatcta
    13921 cctttgagtg gtggccgggg atccggctgc atcactccac cgatctggta cactgggaac
    13981 tgatcgagta tccgctaaac cggacttctc aactggatct gcggggcgtg ggtgcatccc
    14041 agggagtctg ggcaccctgc ctgacttatg ataaaggcac gttttatctg ctgtataccg
    14101 tggtgacggc cttctattgt aatatgtacg atacgaataa ttatctggtg acggcaacgg
    14161 acattcacgg cccatggtcc gaacccatag cgttaaataa tttcggcttt gacccatcac
    14221 tgtttcatga cgatgacggc agaaaatata tggtaagcat ggtaacagat cacagagtgc
    14281 cgaaaaaata tgtgggacgt ctggtattgc aggaatacga tccggtgcag aagaagatga
    14341 ccggaccggt gaaggacatt taccgggcgg ataaaatctt tttggaggga ccgcatattt
    14401 tcaagcgaaa cggatggtac tatctgttct cggcggatac cggcacggga gaactgcacg
    14461 ggcagaccat acagagatcc cgggatatct ggggacctta tgagatgtac caggcagact
    14521 tcatggagag aactgcggag aatgaggcct attccatcct gacttccaga catcatgagg
    14581 atattctgtt acagaaatcc ggacactgcg atctggtgga gacgccggag ggagaatggt
    14641 atgccgtgca tctgtgtgga agagcttcgg accgcagaaa tccggcggat gcgataaggt
    14701 ttgccggagc aagacgttat atgctgggac gtgaaactgc catacaaaaa atgaaatgga
    14761 cagatgacga ctggctagtg atggcggatg gcagtaccga gccgaaggaa tatgtggagg
    14821 aaccggcggg aaaccatagg gtggaaaaac aggtgatgga atccgcacag gaaagtaccg
    14881 gcgcaaatat gcgggatgat tttacggcac cggttctgga tcccgattat cagtccctgc
    14941 gtatccccat gacggagcat tacttgtctc tgacagagcg tcccgggtat ctgcggatgt
    15001 acggaagaag cggtctttca tccaaatttt ctcagaccct gattgcaaaa cgactgacgg
    15061 aatatgatat ggagacatcc acaaagctgg aatttgaacc ggaagtgttc aaacagatgg
    15121 cgggactgat cctgatgtac gatacggata attatctgta tctgcatgtc tcccgggatg
    15181 aggactgcgg aaaatgtatc gctctgttaa aagcagagaa taagaaatac gaatatctga
    15241 cggactatct gcctgttttc ttcggattgc ccatctatct gaaagcggat atgcgaaacg
    15301 gttatattac gttcgcctac cggctggaag gcagcgactg gcagacgatc gcagaaaata
    15361 ttgacggcag ctttatgtct gacgaagcct gcacggaagg gtggtttacc ggagctatgg
    15421 ctggaatctg ctgccaggat ctcactggat tcggcaagta tgctgatttt gactggtttg
    15481 aaatgaaaac aaaataggtt cttaaaaaaa tgtactttga ccttaaaaaa gtgcattttt
    15541 tttatgtctg taaattgcta tattacaatt acaacaacgg aaggccaacc ggaaagtaat
    15601 acataagcac gaaacaatat agcaaggaat caaggaggtt actatgagaa agaaattact
    15661 ggcaagtgtt ctggtagcag caatggcaat aaccatgtta gcaggctgcg gaagcaccga
    15721 tgacaataca acaggaagca ctgctcctgc agcatccacc acagaaagcg cagcaactac
    15781 tacagtagca gctgacgcag aatccatcgt atctgcaccc accgaattaa catttatctt
    15841 tgcagatggt gatgagggag ccaaggcttc catgaatgag atcgttaaca gatttaatgc
    15901 ggcatatccc gatattaccg tgaccatcga gcccggtaat ggcggtgctt acagcgaatt
    15961 catcaagacc aaggacagcg tcggagaatt ccccgatgtc atggagatga gagataccgc
    16021 aatgtatgta agggcaggca agctggagcc tctgtccgag gagattacct ccctgttccg
    16081 caccaccaca gaatttaacg gtcaggtata cacagctcct ttgtccggtg aaaatacaca
    16141 gggcatcatt tacaataaaa aatattttga tgacaacggt cttacagagc ccactacata
    16201 tgaagagttt atgactctgt gccagaccat taaggataaa ggcgatatgg caccgatcgt
    16261 agtcggaggt caggatatct ggcatatggg ctttttgttc cacaaggctt acaatgatca
    16321 ggtactgagc aaggatccgg atttcatcga gcattgctac gagggaacca aggacttctc
    16381 cgatgagacc atgaaggcaa ccttccagga gctgaaggat atcttccagt atgcacagga
    16441 tggctggact tctactcccg atgcccagat cacgactttc ctggtaaacg atatggcagc
    16501 gatgatgtat tccggtacgc atatgttctc ccagatcgat gcagcagatc ctgacttcga
    16561 gtatggatgg ttcgcaattc ccagtcccga tggcaagacc cgtctggtag gtggtgccgg
    16621 cgtcggtggt ctggcaattt ccgcagaagc agccaaggat ccggacaaga aggcagcagc
    16681 agaagctttc ataaagttct tctttgcacc tgagaactac aaggtatact gcgagaccct
    16741 gaacttcatc cccactaccg tagatgagcc tcagatggat gtatctcctg tattccagga
    16801 ggttatcgat gccaatgcaa gtgcagatga ccttgcacct atgtggaacg gtcgtgtagg
    16861 caacaacgag ttgccccccg atttcagaaa ctttacctac aagaccctga ccgaagtgct
    16921 tcagggaacc agagatattg attctgcctg tgatgaatta aataagacct ggcaggtaag
    16981 catgcagagc tttaaccccg ttacaggtgt gggaatcgaa taatccacgg attagagagg
    17041 aatctcgtac tgtcaggtaa ttctgacagc tacgggattc tttttagaaa ggaaggaaat
    17101 gcaatgcaga aaaaaagaaa attcgataaa acagcctttg tgtttatcgc tccggccttt
    17161 atctttttca cactgtttat tatcgtaccg acggtctcta gtgtatatta cagttttacc
    17221 tcctgggatg gcgtaagccc gaatgtaaaa ttcatcggac tggcgaacta taaagagatc
    17281 tttaccagtg cacgttttgg aaatgcattg aagaacacaa tattactgac ggtatttatc
    17341 tctgtattcg aaaatgcatt cgcgttggca cttgcattga tcgttgataa tgtcagatgg
    17401 ggcaagaatt tcttccgcag tgcattttac gttccggttc tggtcagcgg tattgtatcc
    17461 ggtttcatct ggaagatcat gtacaattac aactttggta ccatcaacac gatcctgaag
    17521 gacatcggca tgggtgattt tcgacaggac tggctgggca acaataagct gacactgatc
    17581 atgattggcg tagtactggt atggaagggt gcaggctact atatgatcat ctatctggct
    17641 tctctgcaga gtgtatcccc ggacctgatc gaggcagcac agatcgacgg tgcaagcccc
    17701 tggcagcgtt ttaagtccat cactgtgccc ctgatctccg gtgcatttac catcaacttt
    17761 accttatccc tgatcaacgg attaaaggta ttcgaccaga tcaacgttat gacggacggc
    17821 ggccccggat tcacctccga gacactggta tacctgctgt ataaggtagg cttcaacgaa
    17881 ggcagacagg gcttcggtac cgcagtcggc attatgctgt tgtttatcat cattatcctg
    17941 aatacactgc agcagaaatt cttaagaagc agggaggtgc agatgtaatg agcaaagaaa
    18001 aagttgtcag aaagcccgtg gagaaaatca aacccggaca gatcgtatta tttgtagtta
    18061 ccattctgct ggcgatcctg tttctgtatc cggtattctt cgcactgatc tccgcattca
    18121 agagtaacgg tgatatcctg aagaacccga ttgcattacc gacctccttt tatacacaga
    18181 actttaagga tctgtttgca cagtccgatt tcgctacggc aatccttcat tccgtgttcc
    18241 tgactgtggt atcggaagta ttgatcgtat gtatcgtacc tatggcggca tacgggattg
    18301 agagaagaaa aagtaagaca acgtccctga tctacaccta tttcctggca ggtatgatga
    18361 ttccttttca tctgtacatg tttcctctgt tcaaggagat gaaggttttt catatcttcg
    18421 gtaatatggt gggacctatc gtatgttaca tttccggatc tattgccttc ggtaccctgt
    18481 tatattgcag tttcctgaaa ggcatccctc tggagatcga ggaagcggca cagattgacg
    18541 gatgtactcc gttccagacc ttctggaagg ttaccttccc tcttctcgga ccctgtacag
    18601 gatccatggt gatcctgaac ggtctgggaa tctggaatga ttacctgatg ccttacctgg
    18661 cacttcccag cggcaaagcg aagaccatta ccgttgagat cgcatccttc gtaggacagt
    18721 atactgccag atgggatatc gtatttgcag gaaccgtaat ctccatcgtg cctgcgctga
    18781 tcatcttctg tatgttccag aaatacttcg taaagggaat taccgcagga gcagccaagg
    18841 gttaagcaag ttgcggaatg cattgaaatt tgttattcta taactatgca gaatcatatg
    18901 tcaggattct gcatagttat tttgttacta ttcagagcca tgcaaaactg cgttctgcga
    18961 atggggttct gaatagttac gttatttttt atgatgcagg gtttgcggta gtatggtaac
    19021 gcttcattac cgggacaacc tggtcaggaa ggaaacaacg agaggggtaa ctttattgaa
    19081 acattttaat ctttatcaca aatttgccct gacaatgata ttgctggggc ttttgccgat
    19141 gctccttctg gcaactttta ttgcaaataa aatgatcaga gattactcca gggcattaag
    19201 tgcgcaatat gaccaggcct cggaatatgt attgtccagt atggaatcag tatttgataa
    19261 ttataatacg atatccaaaa tgccttattt ttataatatg ggtgtgggaa atggtgcaga
    19321 cagctatttg tccttcgatc atttccgtaa gattctcaca ggtgaggagt atgaagcgga
    19381 atccatggag acggacagac aacgggatat gcagggattt ctgcagtatc ttcaaagtgt
    19441 ggacaactac attaacagtg ttcatgtcct tgctgatcag gagaatgggc agaggctttc
    19501 ttttcactac agtgtataca ataccttctt ctccaatgaa ggaaaattcg aagaactggt
    19561 gtcctatgaa ggactggaca gggaaaacaa gaatctgata ctgataccgc cccatacaac
    19621 agcctattat tatggcgagc cggagcaggt atttaccata gccaggaatt attttgacct
    19681 gcggggaccg gtaggacata ctccgtatgt agctacgatt tttatagatg tagatttaaa
    19741 gcggatagaa aaaatatttc agagtattga ttttgcaagt aatgagcaga tctatgtggt
    19801 agataagaac ggaaaatgct tttacagcaa tgacgaagag gttatcggaa gtgatctggc
    19861 gcagaagcta caggaaattc aaaatgacaa ggaacggttc gtagtcaccg cagatgggaa
    19921 aaaatacggt ctgtcagtgt acatctgtat ggatgcaagg gtcgcatttt ccgatatccg
    19981 taatatgcag aggttgatgt atggttgtat cttactttcc atcctgttgt tgtttgcggg
    20041 ttccttctat ttttccgcca gactgacaaa acctatgcag cgtctggtga atcagatgaa
    20101 gaaagtggga aaaggtaatt ttgatatcga gatcccggta cagagctcgg atgagattgg
    20161 gacactggcg gaaagcttta atgagatgag ccgggaactg aaaaagtata tcgaccagtc
    20221 ctatctggca cagatccggc agaacgaagc ggaacttacg gcattgaagt ctcagatcta
    20281 tcctcacttt ctgtataata cgctggagat catacgaatg acggcgctgg aagatgagga
    20341 gaagagcaag gttcccgaaa tgatcgaggc attgtcacag cagatccatt acattatcgg
    20401 tcctatgcag gatcttgttc ccttggagca ggaaatcgat attgtaagga aatatgtgta
    20461 tctgttgaac tgcaggatca gtggtaaagt gaagctgatg gtaaatgccc agggagctgc
    20521 catgatcctg gtgcccaagc tgattctcca gcctttggtg gaaaatgcgt atgtacacgg
    20581 cattaagcca aaacagggca gcggaagtat tatgatcgag gcccacagga aggaggatac
    20641 gctggagatt tcagtgatgg ataacggtgt tggcatggat caggacagca tcgataagat
    20701 tcttcagctg ttacaagggg atgctcccgg tatcaagaac cagtataact ggcagagcat
    20761 cggactaaaa aatgttcatg accgcatccg ctatctgtac ggcgaagaat atggaattca
    20821 gattaccagt acggtcgggg taggtaccat gatcagcgtg atattgccat ggaatactga
    20881 ggagaaggga gagagtccag atgataaaga tgatattggc ggatgacgaa ccggtaatca
    20941 ccagaggaat ccgaaaactc atcgactggg aacaactggg aattcagatc ataggggaat
    21001 atgaggacgg gaaaagtgca ttcgcggcaa tcctgcggga ccagccggag attgccctgt
    21061 tagatatatc catgcccgga atgagcggcg tggatattat ccgggaatgt catggcttgc
    21121 aatgcaagac acacatcatt tttatcagtg gatttcagga attcgagtac gcgaaagcag
    21181 cactggatta cggagcaacg gattatctgt tgaaaccggt gatacgggaa gaactgttga
    21241 aagctattga aaaatgcgtg aaaacaccgg agcagccggg cgtaacatat aaaggcaata
    21301 tagatttttc cggggatgcg ggagagcagg aacagatata tgtgccggtt tgtgtctacc
    21361 cgatgttctg ccgcagtgag aacgaacaga tccggcatct gatccacttt tctctgctga
    21421 gttttctgga ggaatatttt gaggagaata aggacggtat tgtgttccag aaggagaacc
    21481 ggatcgtagt cgttctgcaa acgaacggtt ccatgaacgt gtcggagaag ctggaacaga
    21541 tcaaggaggg ggcagagaat gccacagggt atcagatcct actgatctgc ggtgatgaga
    21601 tccattccat gcaggaaatt ccggagatgt tcccaaaatg tgtggaaaaa gaaggctatc
    21661 tgtttttcgc agatcagatg aaatacgctg tgatttcatg gaaggagact ccttttcccg
    21721 gcagaacgga attgcagagt acggaagagg tgaaaagtac aattgcagag gggattctgt
    21781 cacatcagca ggaactgatc cgggacggaa tgacccgtta ccgtcagatg gtatgtcgac
    21841 tgtctaacgg gagaaaagag gacgctctgt tctattattg tacattgatc cagtcattgg
    21901 aagaaattct cagtggaaag ggtgcaaaag tgtcatttcc gaagcatcag gaattgctgg
    21961 aaaaggggag aagctgtgtc agcttttccg agatggaaca gatatttatg gatgtgattg
    22021 ataatgccta taatgggctt ggggaatcag tgacccagaa tgataagaag gatatcaata
    22081 aggcactgga gtatatcgag ctccactatg cagagagttt gtcattgaat gtcctcgccg
    22141 aagaagtaca tatgaatccc tattacttta gcagcttttt caaaaaaagc accggcaaaa
    22201 atttcaagga ctatgtcaat cagatccgcc tgaaacatgc agtcacactg cttctggaca
    22261 ctgacaaaaa agtctacgag attgccatga atgtaggctt tggcgatgcc agagcattta
    22321 acgatgcctt ccagagggcc tacggggaga caccggcagg gtatcggaag agattgcgga
    22381 agtgaaaata gtagttgtgg caatagtttt cgtaagatat aatatatttt gggagagaat
    22441 ttcggagaag gtcttgtaga aaggataaga ccttatgcat aatacactga aaagagctgt
    22501 aatagcgaca aatcaaaagg cacaatatga tgcgaaagtc aaaaatctgc tagggcataa
    22561 aattatactg gctcatatac tgaccagaac agtagatgaa ttcaagggga tgaatcccag
    22621 ggaggtgatc ccctatatag aaggaacacc ttatatcagc aaggtgccga tagaaccggg
    22681 gatgaccaat cacgaacaac agggacagca gattatcggt ctgaatacgg aaaacgtgga
    22741 aataaacgag ggactgataa gatttgacat tattttttat gtccggttac catctgaaga
    22801 tagcaaggat gccggattga cacaaatcat cataaatgtg gaagcacaga aagatgaacc
    22861 gacgagttat gagattttaa atcgtgcgat attttatgcg agtcgcctgg tatcatccca
    22921 aaaggagcgg gattttgtta aatccggtta tgatgatatt aaacgagttt attccatttg
    22981 gatatgtatg aatatggaac agaacagtat gaaccatatt cattttgtaa atgatgctgt
    23041 aatcgggaac catgaatgga agggaaaaat cgatctgatc aatattataa tgattgggat
    23101 tgcaaaagaa cttccggaac aggatgagag atatgaattg caccgtcttc tgggagcact
    23161 actgtcgcag aaactgacag tagatgagaa aatgtcgatc atggaaacag agtacgatat
    23221 tgagttggaa gaaaatttgc gaagggatgt gagtacaatg tgtaatttga gtgaaggaat
    23281 cgaagaaaag ggaattgcaa tcggtgaagc gagagcagaa gcaacaatga tagccaggat
    23341 gcataaaaac ggaatgcaac cggagcagat tgcggcagtc acagagaaaa gcattgagga
    23401 agtaaggaaa atcatagctg attgtgagaa tggataaagc aaaagtgata caggaatggc
    23461 agatcgctgc tgttcctgtt tttttctcaa attctttctt ttaactttat tgcagtgatg
    23521 cgattgagtg tatataatag aatcgttatg taatgcagta ctttagagaa ggaataaatc
    23581 acaatggata ccatcagagg taaatttttg aagcaacatt tactctcaga cgagtttttg
    23641 gatctgatcg agaagaataa gcgccccatg gacgagctga tgtcttatta tcagtgtgcc
    23701 atcatggagg tggagacgaa attcaaggtg ctgaatcagg agtattccct ggaatatgac
    23761 cgcaatccca tcgaggggat caagacccgg gtgaagtcct acgacagcat ccttaggaag
    23821 atccgccgga aaaatattcc catgacgctg gaaggcattg aggagaatat ccgtgatatc
    23881 gccggtgtcc gggtcatctg ctctttcccg gatgatatat acgagctggt ggagagcttt
    23941 ttacgacagg atgacattac cctgatcgag cgcaaggatt atataaagaa tcccaaggag
    24001 agcggttacc gcagccttca tctgatcgtg caggtgccaa tctttttaca gaacacgaag
    24061 aagcttgtct atgtggaggt gcagttccgc accatagcca tggatttctg ggcaagcctg
    24121 gagcacaagc tgcagtataa gaaaaacatc ccggaaagtc aggcaaagtt tttaaaggat
    24181 gagctgtatg actgtgcaca gcagagtgcg gcactggata agcggatgca gaatatccgg
    24241 aatgtcattg cagagagtga gactaaggaa gaggaaaaac aggatttcct gccgatcttt
    24301 ttgcgggaaa acaaagggta attacgcagc ctgaaggtat gtagatgatg gaaaggcgag
    24361 aaaaattgta tgaagatcag agatattttg aataaagata tagcaacgat ctcttttgag
    24421 gtatttcctc cgaagaagag tacggatttc ggaaatgtgg aggcagcagc actggggatt
    24481 gcggcattgc agcctgctta tatgagcgtg acttatggtg ccggtggcag caccaagggg
    24541 cataccattg ctttggcagg agagattcag aagcagcaca atgtgcctac ggtagcgcat
    24601 ctgacctgtg tctgtgctga caggagcagt atcggcgcag ctcttacaga aatgcaggcg
    24661 gcgggaatcg agaatattct ggctctgaga ggagatattc ccaaggattt cgacggagaa
    24721 gtatttacgg attttacgca cgcctccgac ctggtgcgtt ttatcaaaga aaacggagat
    24781 ttctgcgtgg gcggtgcctg ctatccggag gtgcatcctg attctgcaaa taagaatgcg
    24841 gatattcagg ggctgaaggc gaaggtggat gccggatgcg agtacctgac gacccagatg
    24901 ttcttcgaca acaatatctt cttcaactt
//
LOCUS       DAWEJW010000030        24734 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_828_length_24734_cov_59.223267, whole genome shotgun sequence.
ACCESSION   DAWEJW010000030 DAWEJW010000000
VERSION     DAWEJW010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 24734)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24734)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24734
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_828_length_24734_cov_59.223267"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    22304..22403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7103 a         4914 c         6089 g         6528 t
ORIGIN      
        1 agggcgaaaa tacggaaaag ggtactcttt tcctatgctt gctaagaaag ttttttatct
       61 tttatgtcct tatttccctc tacggagaaa aaaccatttt gccgaactta tgcaaaagaa
      121 aaacgctgca atctcaaaaa agattacaac gcttccgaaa atccatattc aattttgccg
      181 gacgtttcta tttgtcctct ttttcgactt ctgaaatttc ctttgcggtt gctgttacaa
      241 tccgtatgcc gttatcgctc atatcatcaa gcagagcgtc gagctgccgc cgctttgtat
      301 cttttccgac gggcgtatct aaaagaaact ggtctacgga tatattgtaa cggagcataa
      361 gctcaaaaaa gatttgcaga ctggggtgct gtcctttatt ctcaatattc gcaaggtagc
      421 gaggggaaat atacatttca tcacttacct gtttgcggct ttccttgcgt cctgtccgcg
      481 cagcttttat cgcttctcca aaggctttga agtcatattt ttctactggt ctttttgcca
      541 tatttattca cccagttaca ttttactgtt cccctttctt cttggatatg tctatacagt
      601 tccactaatt agccataata attcatattt atatacttgc tattattcgt atgtccgact
      661 ataattatac tgatatgatt tgtagaaagg acttgggtgt tatggaatat atgtcttgcc
      721 ccgaagcagc gaagaaatgg ggaatttctg aaaggcgggt acaggtactt tgcagagaaa
      781 accgcatacc gggtgtttca aaacttggat atatgtggct gataccaaag gacgctgaaa
      841 aaccgattga cgggagaaca aaacgaggaa aggagctgca ccatgaataa agttttgatt
      901 atagatgatg ataaagagct ttgcgctctt atgaaaaagt gcatagagca ggaaaaatta
      961 tcagccgtaa ctgtatataa cggtattgag ggggttcgtc taattgatga aaataaagat
     1021 agctattccc ttattatttt ggacgtaatg ctgccggata tagacgggtt tcagattctc
     1081 caaaaaatta gagatacaag caatatcccg gtgcttatgc ttactgcaaa aagcagcgaa
     1141 gaagataagg ttttcggtct gcgactggga gcagacgact acttgacaaa gccatttggt
     1201 attaacgagc ttttggcgcg tgtcaattcc cttatccggc gatataccac cttaaacccc
     1261 tttacggcag acatagacag catatcgttg aaagatatgg taattgacaa attaaatcgt
     1321 acagttacgg tcaaagatat tcccgttttg cttacaggaa aagaatttga tttacttctc
     1381 tttcttgctt ccaacaaagg acgagtattt accaaaaaac aaatttattc acaggtatgg
     1441 gaggaagaat acgcttttga tgatagcaat attatgtctt ttattagcaa attaaggaaa
     1501 aagattgagc cagaccccga ccaccccttt tacattttaa ctgtccgggg tgtcggctac
     1561 cgttttaaca aggaggcgta acatggatat aaatgtttat cttttcatag tgatagccct
     1621 tatgctgctt attattatgt atctcctttt tgtcttacgg cgtgtgcgga cacagcttgc
     1681 gctgataaaa gatgccttag atgatataaa gggcgggaat ttgaaccgac gtattttagc
     1741 aggcgaaaac gatatgacaa aaacaatttg ctatgacatt aacaaaatag cagtaaacag
     1801 tcaggcgcag cttatacggc aaagacaatc tgaacaggca tataaacggc tgatgacaag
     1861 tctttcacat gatgtaaaga ccccgcttgc ttctcttgta ggttatctgg aagcggttga
     1921 aaacaaaatt gttattggag aggaaaagga cgaatatatc catgtcgcac atgaaaaagc
     1981 ccactatatg aaatcctttg tggaaaattt gtttgaatgg gtaaaactgg attccggaga
     2041 gcagattttt cattttgaaa aatttgacct taacgagtta tcccgcaaca ttatagcgga
     2101 ttggattccc gtcttagaaa gcaatcattt tgaatatgag tttgtcatac ccgaaacaga
     2161 gtattttatt cacatagatg taaacgcata tacccgcgta cttaacaatc tttttcaaaa
     2221 tattattatc catagtgcgg gagataaaat ggcactgcat atttgtgaag atatacaaca
     2281 agctaaaatt ataatttcag ataatggaaa aggaatagct cccgataagc tgccacatat
     2341 ttttgaaaga atgtaccagt gcgaccagtc gcggtctgca aagggaaatg gtttaggact
     2401 ggcgattgct aaagagctta tcagtgttca caaaggaacc attaccgctg aaaacagctc
     2461 ttataaaggg actgtattta ctatattgct tcccaaagcc ctgtaaataa tgcggggctt
     2521 tcctgctata aaacaagaaa aaagcaaggt ttcggcaagg ttatggcaag gttgatacta
     2581 tataatggga ggtagaaagg aggaaaagac aatgactatc atttctactg aaatgctgaa
     2641 atggaaacgc aataagacag tcttaggtat ctttattcta actgcaattt taggaatatt
     2701 cgcagttgaa cgtgctttca gtatatcaag aagtagctca ctaatgaata gttttagtga
     2761 tttatacaca ttggcattta agaaccttac aagtttgttt ctgcctattg tgttgagtat
     2821 gtttgcaacc acattgttct ttgatgaaca gaaaaatgat accttaaaag gacttctgat
     2881 tattccgatt tccaaaacgc aactttattt ttcaaagata gcagtggtaa tttttatgtc
     2941 attagcacta tgtctgctta cttttgcaat ctgtattctt ggcggctttc ttgcaggcgg
     3001 ctttacagat ttgaatacgg aaacaatttt acaggcggga ttattatttt tagccggtgg
     3061 cgtgttagtt ccacttgcaa tgctccccgt tattttttta gcaacattgg caaagggata
     3121 tgttctaccg attggggctg cattacttta tcttgtgcct gtcgtgctta ttccgtcggg
     3181 gattatggga ctgcacccac tggcgagtgt tatgggaatt tatccctata tttcatctga
     3241 cgcagcagaa atggttaaga aatgggcggg aggaatagaa ataactatat ctccgaatat
     3301 ctgtttaatt tctcttttgg taattggaag tatttcagcc attgtttccg tacttgcatt
     3361 gcggaaacag acctattgaa agtgaggata tatgatgaat aaatatgtaa ttgaaacaaa
     3421 acaactgaca aaaacttatg gagagcaaac ggtagtcaat tctgttaata ttcatgtgaa
     3481 acaaggacag atatatgggc tgttggggcg taatggggcg ggtaaaacca caattatgaa
     3541 aatgattttg gggttgaccg atattacttc cggcgaagta gacgtattcg gtcaaaatat
     3601 caaagggagg gaaaaaagag tatatccccg gattggtgct attattgaaa cacccggctt
     3661 ttatccgaat ttaacaggta cggaaaactt ggagatattt gcaaaactgc gcggtactgc
     3721 tgcgccaaat gcagtaaaaa acgccttgga gattgtcggt ctgccttatc gggacaaaaa
     3781 attgtttagc aagtattctc ttggaatgaa gcagcgtctt ggaatagcaa atgctatttt
     3841 acatgaccct gaactgctaa tcttagatga acccacaaac ggtcttgacc cgataggcat
     3901 tgcagaagta agggacttta ttaagacttt aagtatcgag cgcggaaaaa caattctcat
     3961 ttccagtcat attctgtccg aaattgcact actggctgat gacatcggca ttattgacca
     4021 tggtgtacta ctggaagaaa acagcatgaa tgagctacaa aagaaaaaca gtaagtatat
     4081 acttctgcaa gtttctgata taccgaaaac ttctcttgtt ttggaaaggc agtttggggt
     4141 taccgaatat tccgtacagg acgaacacac cctgcggatt tatgacactt cgttggatat
     4201 ggctgctgtc aataaagctc ttgtggtaca ggatatagct gttatcagtt ctcagctatg
     4261 caatgataca ttagaggact atttcaaaaa aatcacagga ggagaaggaa ttgcttaggc
     4321 tgatacaggc agagtttctt aaactgcgtc gaaaaaaatt tattgtgtta atgctgttat
     4381 ccgctctttt catgccactt cttgcagttt tttatttcag aagtgctgat aaaacagtta
     4441 ttgaaccatt acaattttat aagtggacta cattcagcta tacagcttgg attatacttc
     4501 ctgttgtatt gggtttggtt tctactatgc tcatgtatga cgaaaatcaa aacgatatgc
     4561 tcaaacagtt gtggattata ccgattagca agacaggata tttttttagc aaatttattg
     4621 ttgtactgtc ttattccgtt tgttttatgt tgcttacagc catagcgtct gtatttttcg
     4681 gtatgcttcc cggttatata acctttgaat ggaatagtat cagttatcta tttaagaaat
     4741 gtattgaaat cgccatactt accgcatttt ccatattacc acttttagcg gttgcagctg
     4801 ccgggaagaa ctatatactc cctgtatgtg tgacactggt ttacaccttt cttagcttta
     4861 ttttgcttat ggttaatatg tacctacacc ctctatccag tgcaacagca attatcatgc
     4921 gagatattcc cggtgttatt ttggcacagg aagtcaatat tccggctgca cttttttgta
     4981 tcggtatatg gactattgtt tctatcgtat ttgcgtacat ttccttaaag catagaaagt
     5041 gagtgattga atgatgaaag aattgatttg ggcagaacat caaaaactcc gacgttcaaa
     5101 aattgtgttg gtcgccatat tcgcagttat tatgattgca attattgttt ttactcaagg
     5161 gcagttttct tttggggaaa aacgctatat agatgacgca gggtggttta tgaacgcagc
     5221 acagtcttta gcaacttttt ttgttcttcc tgcggttatt gcacttttgg ggagctatat
     5281 gatttgtcgt gaggaacaag aagatactat taaatcatta cgtttaattc caataagtga
     5341 agctaaaatg acgattgcaa aattacttat tacttttgca tttagcgtac tcttttattt
     5401 gctgcttttc ggcattacat ttttagttga agttattttg cattttaggg gcttgtctat
     5461 caaaatgatt ttaagttttt tcaaaatcta tttacttgac ggcattggga ttttctttgc
     5521 tattgcacct gttattgcgg ttgtatcaag aataaagaaa ggctattggc ttgctttgat
     5581 ttttacagta atttattctt ttgcaggatt attcacaagt atatccgatg ttctttcggc
     5641 ttgctatcct attactgcta cattcaatat ttccggctat tattctgcca cctttggaga
     5701 aatcattatt agccttatga gtttgttctt ttgtggattg ttttctgtct tgattttaat
     5761 atctaaaaaa acaatcaaaa ataagtaatg atacaaccaa catctgccgg acgacggcaa
     5821 agaaaaaaag ccgtcgcaga gcagccatat tttcacgcgc ctattctatt tcggcggctt
     5881 tgattttcag agccgccgtt tctttttgtc tgtacgacga cccttaacca tgtagacatg
     5941 gcggctttag gaggtagccg ccatgagtta taaaccacat caacaaattc ttacattctt
     6001 tgatttatca aaaaaagccc gaccgccacc ggggatattc ctacccttag atatgaacta
     6061 ttaaatcatc taaatacttt ttattattcc tatgcgcctg tccgcgtttt tttgcggacg
     6121 ggcgcatttt gtatgcttaa aaaaattttt gaaaaaattt ccgaaaatat ttggcatttt
     6181 ttcaaagctc cgtattaccc cgcgaaaagg gggaagcacc accaactttc aacgagaaag
     6241 gaggtgagaa tgtggaacct aatagcaggg agttttataa gcaatgttct tttcagaagt
     6301 attgcaatgc ggtattgcac aatgaagcgt gcgacgctca tagagagctt cgtacacata
     6361 aggcaaagga agtaaccttt tccgacatga ccttagatga agcgcgacag ctacatacct
     6421 ttgatgaata tttcaaacgg gaaactgctg aaaccgtctt tgaaaaagcc ggaaagaaaa
     6481 tcacgccgaa gctgcttctt gaagcaatcc gtactttgcc ggaagaaaag cgcaaagcca
     6541 tattgctgta ttatttcgag ggaatgagcg atacagaaat tgcagagttg ctcaatacac
     6601 cgagaagcac aatacagtac aggcggacaa gctcttttga acaattaaga aaatatttgg
     6661 aggaaaatgc tgatgaatgg gacgaatggt aatcaacctg gctacccgga aaatgccctt
     6721 gttccttatc ctgtcattgt ggcagcgaca aagggcgacc cggacgctat gaaaatggtc
     6781 ttgcagcatt tcagcggtta tatagcccgt ttatccatgc gaaagttata tgacgagcgt
     6841 ggaaatgttt attttggtat agacaacgac attcgggaac ggctgcaagc caaactgatg
     6901 atggctgtcc tcaattttag aacagagaaa taaccgcaat cggcggtgga acgtctttac
     6961 tccccctttt ccgttccgct gctaatgcgg caacacagcc attttgaacc ttgacaaaga
     7021 aagcggcgca atagaacggc gacgcagcat aaggcaaatc ggatacgttc cttttgtgcc
     7081 gagccatacg gcgggtgcgc catgaccttt ctcctacggg aaaagcgagc gaacaacacc
     7141 accgctgcaa agcaagtgta gcaacttgcg ggcgataacg cacagaatac ataatgatac
     7201 ttccttacag ccatagtccg agcgttagaa gcgtcgcagg caatgggtag ggcttcggca
     7261 gaccgccgga ggggtgcaag tcccatgagg ttacgctaat agccgtccga ttaaagccta
     7321 tgttacaaat tgacttttga cccatttaga aagggggacg acaaattgaa caataacgac
     7381 gtgccgattt gggaaaagta cactcttacc atagaagaag ccgcaaaata tttccgtatc
     7441 ggagaaagca agctgcggaa actggcagaa gaaaatcccg tcccggactg ggtaatgatg
     7501 aacggaaacc gtattcagat taagcgtaag cagtttgaaa aaatgattga tacggtggac
     7561 gcaatctgat agtgaaatcg agccttgaat gtggtacaat gtagtcaagc atatcaaggc
     7621 tctttccatt atggaaagga gcataacgat gtcggaaaaa agacgggata ataaaaatcg
     7681 tattctccgc accggagaga gccagagaaa agacgggaga tatgcctaca aatatataga
     7741 tacctttgga aaaccgcagt ttgtgtattc ttggaagtta gtacctacgg acaaaacccc
     7801 ggcaggaaaa cgcgacgata tagcgttgag ggaaaaagag aaagaaatcc aaaaagacct
     7861 tgacgacggt atcgaccata tcggaaagaa aatgacggtc tgccaactct acgcaaagca
     7921 gatacgccac cgggcaaatg tgcggcatgg tacaaagcag gggcgaaaac agctcatgcg
     7981 gattttgcag gaggataaac ttggagcctg ccggattgaa aatgtgaagc tctccgacgc
     8041 aaaggaatgg gcattacgca tgaaagaaaa aggttacggc ttcaagacta tcaacaacca
     8101 caagcgttcc ttaaaggctg ctttttacac agccatacag gacgattgta ttcgtaaaaa
     8161 tccatttgac ttccagttga acacagtcct tgaagatgat acggaaccaa aggaacctct
     8221 atcccctacg caggaagcgg cttttctgtc ctttgtgcag catgataaag tctatcagaa
     8281 atactacgac gagattatca tactgttagg gacagggctt cgcatttcgg aactctgcgg
     8341 actgacggaa gctgacattg acttagacaa acagcttatc aatgtagacc accaactttt
     8401 gaagattgca gacgtgggtt actatgtgga aacgcctaag acgaaaagcg gcaatcgggt
     8461 tattcctatg agtgaaaaag tgttggaagc atttcaacgg gtgctgaaca aacggaaata
     8521 tgcacagcct gttattttag agggctacac aaagtttctg tttcttaacc ggaacggctt
     8581 gccaaaagtg gcagtcaact atgaaagtat gttccggggg cttgtaagga aatacacaaa
     8641 aacgcaaaag gtagcattgc caaaggtaat gacaccgcac accttacgcc acacattctg
     8701 caccacgtta gcaaatgcag gaatgaaccc aaaggcattg cagtacatca tgggacattc
     8761 caacatcaat atgacgctga actattacgc gcatacgact tccgaaacgt cgataacaga
     8821 aatgaagcgg ctgattgcat aagtagtaaa cggagaattt actactccac atactacttt
     8881 tgaacaccaa aacatacggg gatataagaa gatttgagaa tatcttccat aacaaaaaat
     8941 gccggaaaag cctgtaacaa taggctatac cggcatataa gaacttttga aaagatagtc
     9001 aaaaattact ttgtgatttt acataaatta caggaaagac actttctgct ccgcaggaaa
     9061 gcagaatccg cacgatatcc tttagaaaac ttttcagata ggtttcttct acaaaggaac
     9121 agatacaggc atatagtccc ttttcgggaa aggacagaag tccctgatac tttttgaata
     9181 cctggggaaa attatcaggc tgatccaatg cttccataag cagcccctgt tctaatggcg
     9241 actgcatatc cttcagcaga agggcctgct ccgccttctg ctgtacctct ttttgctttt
     9301 tttcttcacg gatcgtatcc agacagttga tcagctcctg cttgtccgtg ggttttaaga
     9361 gatagtagcg gaccccgtat ttcatggcct gcttggcata ttcgaattca ccgtaaccgg
     9421 ataacaggat aaaagtgatc cgggagtcga tgcgggaggc acgttcgatc agttccagac
     9481 cgttcaaaat aggcatcttg atatccgtga tcacaacatc gggatagtca tcacggatcc
     9541 ggtcataggc ttccattccg tttttggcgg tagcaatcac cttgtagccg agggaggtat
     9601 aatcgatcat ggaagaaagt gcttcacgta tgatctgttc atcatctaca atcatcaatc
     9661 tgagcataag aatcctctct ttctttggga atggacagca gtacaatggc cattccgtct
     9721 tcattgtata tagataagcc gtaccggtcg ccgaagagca gcttcaggcg ggagttgata
     9781 ttcaccagtc ccacaccgga accctgggga gtgacctgcc tgtgttccag cttccagatg
     9841 agatcatcct cgaaatggga accggtattt gctacggtaa tctggtagac ggtgtctttt
     9901 tcggaaatgg tgacggtgat atgacagaca tcgtcactga attccagagc atattttaat
     9961 gcgttctcta ccagaggctg gatacaaagt gtcgggacat aaatattgga aatgtcatcc
    10021 ggcacatgca gctggaagtc cagccggtct ccgaaacgga tctgctggat ccggatataa
    10081 tcctttaaaa cgtccagttc cttttttaaa gggatcaggt cttctttgct gttgacagca
    10141 gcgcggaaca gattccccag ggaacggacc atggtggaga tgtcatcggc accgtatttt
    10201 tgcgccatcc agttgatggt atccagggta ttgtagagaa aatgcgggtt gatctgctgc
    10261 tgcagcatct tgatattggt atcccggagc aacagctgct tttcatagtt tttatcccgc
    10321 aaagtcttta cattggattt catttcgtca aagctgacat gcagctgccc gatctcatcg
    10381 gtacggagac tgtaatcgta gtgagcggtg tcctccggat cctggagtcc ctgtccgaag
    10441 cggcggatct tctctaccag aagatccaga tggtgcagta tcctgcgcag aagcaacagt
    10501 gccagaaaca gagcgatcat cacgaccacg gcggagaaca ggatcatccg gatcagaacg
    10561 gtatggatcg cagaaaagag cggatcgtaa tcctgaaaat acagataatt ccatccggta
    10621 cgtggaaaat ctccctggat gacaaaggtc tttcgtttat ctatggaaag aatagaataa
    10681 tggctgctgc catgattcag cgactgcagc aggctggaat actgttccgg atggtaagtc
    10741 tcctgtgggt aaaactgcgc atttcctgca aacaggataa aacgggaatg cgtggagaga
    10801 gaatatccgg cattctccag ggcatcactg atcagctggt ccaggtcgat cactatgtac
    10861 aggtaagcca gatgatccag ggtcagatgg cgaagctgcc ggatatcccg gtaaagtacg
    10921 atggtattcc cgggggaagc cggtgcactc cagctggcac gcccctggca ggctgctgca
    10981 ttttctgcca gttcctccag aggaaaagca tccagatcgg aagtatttcc catacagatc
    11041 agtgtgtcgt caggaagtat cagactgatg gatttgatat agtcgtcatc aaaaatataa
    11101 ggatagagag cctgatatat cgtctgcttt ctctgggcat tacggatact ggtgggagca
    11161 tccatgatat cggtgagatt cgtctgaatg atggtatcgg acaggatccg ggcagaaccg
    11221 gattccacgg tctccattct tgcagagacg aaggaagata cataattcag agactgtgca
    11281 ttggaagtat acagctcacg ggcataatac tgggaaagaa gctgaatgcc cacaacaaaa
    11341 gcaaagaaca caaaaagcaa tgtcgttgac aggatcagta gtattttctt atataaagac
    11401 agattattaa aacgctgtga taaattgctc atggaaaaac ctccgtatca tggttattat
    11461 atagtaaaaa tatgggaagt tcaattttca tccgtgggaa gaaaacaaaa agccgttttt
    11521 tgaacactca gaaacctttt tttgaacacg aaattatgca aaatgccata tggaatatgg
    11581 aaaaactgaa aaaagtatga aaatgtgacg aaaaaaatgt aagattatgg ttaaaaagcc
    11641 atttttttgc atcgacagaa ccgttattta actttaccat tatgcaaaat ggacataaaa
    11701 taaattcatc aaaacaacaa cgagggatgt gcaggacaca tacccgacag attgtatggg
    11761 aggtataaca aatgaagaaa agaatgattg caggtcttat ggctgtattg atgaccatgt
    11821 ctctggctgc atgtggaagc agcgcaggca gttctgatgc atctggcaca ggctccgctg
    11881 ccacaggtga taacagcgca gcacaggctg ccagtggtga caaagtaacc ctgagccttt
    11941 gctggtgggg caaccagaca agaaatgatg taaccaagaa ggcagttgac ctttacatgg
    12001 aacagaaccc caatgtagag atcaaggtag agtttaccga ctggtccggc tactgggata
    12061 agctttctgc aatggcagca ggcggcaacc ttcccgatat tatccagatg gattactcct
    12121 atctgcaaca gtatcagcag agcggccagc ttgctgatct gagccagttc attgacagcg
    12181 gtgtcattga caccagcaag attgcagaca gcgttatcga tagtggatct attgatggaa
    12241 cctgttatgc attaagcctt ggcagtaatg ctcctatgat ggtatatgac aaagaaatcg
    12301 tagaacaggc cggtgtagag cttcccgagc agctgaccat cgaagaactg tatgatatcg
    12361 gacagaccat ttacgagaag accggtgtga agacctacta cgatggcggc atcaatatga
    12421 tgcagatcat cgcaagaacc cagggatctc atttgttcga tgaactggca gcaggaacca
    12481 agactgcaag cgaaacccac ttcgctaatg tagataagtt caacaaggcg gaatccgcta
    12541 tttctcctga tcttctggca gagaagaacc cggatgtagt agagaccaag cccatcattg
    12601 atggaaccac atggaatgac ttttcttatt ccaaccagta catcagtatt gccaacacag
    12661 ccggtagaga tctgggaatc accatgtatc ctactaccag cgatgcaacc acccaaccga
    12721 tgttcttaaa gcccagccag ttcttttcta ttgctgagac ttcccagaat aaagaggaag
    12781 ctgcaaaatt cctggactgg ttcaccaaca gtattgagtg caacaatatc ctgatggcag
    12841 agagaggtat ccctgtgaac agtgatgtag cagaagcgat caaacctaat gtggatgaga
    12901 attcccagaa ggtatttgac tatatcgcag aagtatccaa gatcgcaact cctattgatg
    12961 atcccgatcc ctctggtaag ggcgaaatag aagcacaggc aaagaccatt gttgagaacc
    13021 tgcgttatgg agatacagat gcagcgggag cagcagaaga attcgtaacc acatctcaga
    13081 agattctgag cgagacggca aagtaagacg agaagtaaga cttctgaagt aagaccgtta
    13141 taacaaattg taatcaaagg gcgcctggat cccatccggc atcgggcggg aaccgggcgc
    13201 ttgttataga aacggcagaa ggagaggaaa aatgagcaaa gaaaagactt ccccaaagag
    13261 aaaaagaaca ttgggccagt ggctggatag tcctaacgta tccggatatg tgtttgtcgg
    13321 accgttcatt atcggatttc tggcattcat gatggttccc atgcttttat cttttgcatt
    13381 ttcatttaca agatacaata ttttacagtc cccgacattc attgggctgg aaaactacat
    13441 taccatgttc acggatgatc cgaaattctg gaaggtattc ggtgtcacca tgtattacgt
    13501 ggtattttcc gtacctcttc gtctgatcat ggcattgatc gtagcaatgc ttctagtgaa
    13561 gagcaccaag ctgtccggct tttacagagc agtgtattac cttccttcca ttatcggttc
    13621 cagtgtggcg gttgccattc tgtggaaaag aatgttcgcc agtgacggtg tgatcaacgg
    13681 tctgttcgga ctggatatcg catggctggg acgttccgat actgcgatct ggaccctgat
    13741 cattttggca gtatggcagt tcggatcctc catgctgatc ttcctgtcag gattaaagca
    13801 gattcccgta agcttatatg aggcagccac catggatgga gctggcggtg tccataagtt
    13861 cttcaagatc acactcccca tgctgacccc cacgattttc ttcaatctga tcaaccagtt
    13921 gatcaacggc tttatggcat ttacacagag ctacattatt acacagggaa aaccaaggga
    13981 taccaccctt ttctatactg tatatatgta ccagaacgca tttacctata acaaacttgg
    14041 ctatggatgt gcacttgcat ggtttatgat tctggtagta ggaattttga cactgatctt
    14101 gttcaagacc cagaacaagt gggtatatta tgaatcagaa ggttgatggt gagcgtatga
    14161 aaaaaggata taaagaaaaa agatttgcta tcggagtgat ttatcatatc ttcaccttct
    14221 gttttgcctg cgtgatgata tatccgttag tatggatggt acttagttct tttaaggata
    14281 ccaatgagat tataagaacg gcatcggaat tgatcccgag acacttctct ctggagaact
    14341 acaaggtagg ctggcaggga tttgccggat acaatttcgg aagcttcttc aaaaactctt
    14401 ttgtgatcgc aggtttatcc accatcggag cagtggcttc ctctgctgtg atcggatacg
    14461 gcttcgcaag aattaacttc aaattcaagg gattctggtt cgcctgcatg ctgttgtcca
    14521 tgatgcttcc gttccaggta gtaatgatcc cgcagtacat aatttttaat aaattaggct
    14581 gggtcggttc ttaccttcct atcattgttc cccagtggtt cggtcaggga ttcttcattt
    14641 tcctgaatat ccagttcatc aaggggattc ccatggatct ggatgaggcg gccagaatgg
    14701 acggatgttc tacctatacg atcttcttcc gtatcatcct tcctctgatc aaaccgtcac
    14761 tggtaaccag tgcgatcttc tcctttatgt ggagatggga tgatttcctg gcagcactgt
    14821 tatacttaag cgatcctctg aaatatccgg tgagttacgc tctgaagatg ttctcggatc
    14881 ctaccgcagg ttctgactgg ggtgctatgt ttgcaatggc aaccttgtcc ctgattccta
    14941 tatttgttat tttcctgact atgcagaaat atctggtaga aggaattgca tccaccggta
    15001 tcaagggcta aagctgttcg gcggggagac tgttccggtt ctccccgctt ttcttgtttt
    15061 cagataaaaa tcagaggaga gtgtgatatg gacactgtta gaaatcgaat caaagctgtt
    15121 ctcgaagccg tagagcagat gatatatagt gacagacaac cggtaatttc cgtgaagatc
    15181 gccaaagcgg attaccattt tgaggaggaa ttccacagag aaggaaaaga tttccgggat
    15241 ttcggccgca atgagacctg gggagagctg gatcagcatt atttcttcct ggcagatgtg
    15301 gcgatcccgg agcagatgga ggggcataca gtccgtgccg tgctgaatac cggagccaca
    15361 gatatctgga atacggacaa tccccagatc atggcttatg tgaacggcag atttgcagcc
    15421 acgttggata tgaaccatca gtttatcatt ctgtcggagc atgccaaagc aggagagact
    15481 tacgaactgg ctttttatgc gtattccagt gcgtatgcag gcacgtttac cggtacgaat
    15541 ttcttccgcc tggagctggc agtctaccgg gaagaggcgg cgggactcta ttatgacatg
    15601 caggctgtct atgaagctgc ggatcttctg ccggaggaca atctatccag gatcgagggc
    15661 tttaaagcac tggaaagatg cataaatctg ttggatttgc gcagacccgg atccgcagaa
    15721 tgctttgaga gcatgaaaat ggcggcgcag gatctggaag gtacttatta tgccgacaga
    15781 agaccgaatc cggtgacggt gcacagtatc ggccataccc atattgatgt ggcatggaaa
    15841 tggcctctgc gtcagaccag acaaaaggca gtgcgcagct ttgagacggt actgaacctg
    15901 atggacagat atccggaata ccgttttatg tccagtcagc cacagctcta tgagttcgtg
    15961 aaggaagatg ctcccggggt attggccaga atcagggaac ggatcaaaga gggcagatgg
    16021 gaagcggaag gggccatgtg gctggagccg gactgcaaca tttcctccgg agaatccctg
    16081 atccggcata tcatctatgg ccgcagattt tttgaacaag agctcggtgc ggagaaaaat
    16141 gaagtattgt ggctgccgga tgtctttggt tacagtgcgg cgctgccgca gatcctgcag
    16201 aaatccggga ttccttattt tatgaccacg aaaatcggat ggaatgagtt caaccggttt
    16261 cctcacgaca cctttctctg gaagggcatt gacggaacgg aagtgttgac acatctgatt
    16321 tctaccagaa actatcagaa gccgggtgac ctgaagatgg tgggaaacca ttccaccact
    16381 tataatggtc tgcagaatgc ctcccagctc atgggtacct ggcagcggta taaggataag
    16441 gatgtgagca ctgaggtgct gacctgttac ggctatggtg atggcggtgg aggccccaca
    16501 gaagagatgc tggagcagag cagaaggctg gagcacagca tcgtggaatg tcctgcagca
    16561 agacagacag gggtgaagga attctttcat attctggaag acaaaatgga caaaaagcgt
    16621 ctggcggtat gggatggtga actatatctg gaattccacc gtggtactta tacttccatg
    16681 gcacagaata aaaaatataa tcgtaaggct gagttcaaaa acggcgaaac cgagctgtat
    16741 gcagcaatgg catcacttct ggatcaaaag tatctctatc cacaggaaca actggaacac
    16801 agctggaaac tgcttttgct gaaccagttc cacgatattt tgcccggttc ctctattaag
    16861 gaagtgtatg aggattccgc cgcacagtat gaagagattt ttgctgcgga tgaagaaatg
    16921 atgaaaacag cgaagaagag catccgggaa aagctgttcc ggtatcgtgc ggagaagaat
    16981 gaggaggtct gcgcagtatg gaatcctttg agctttgcac gtacggcact gatccgtaat
    17041 gcagagggca gttggcagaa aataacagca ggacccagcg gtgttactgt gtgcagagcg
    17101 gtgaactccg gagaggacaa ctgtttcact gagcttgtaa tggaggaaaa cggacgaccg
    17161 gtaagcttca aaacgcccta ttttaccgta agatttgatg ccaatgctga gatcacaagc
    17221 ctggtggata tcagggagga ccgggaactg atccaaaagg gcaggatggg taaccgtctc
    17281 accgtatatg aggatcggcc ggcagaattc gatgcctgga atattgacga gggatatctg
    17341 gagaaatcct gggatgtcat tgatgtggaa gaattcaaag tattggagaa cggaccggag
    17401 acggcggcat tacatgtgcg cagacggttc caggattcca tgatcgaaca gaccattcgt
    17461 ttctaccggc acaccgcaag gatcgatttt cagaccagag tggactggca ggaacatcaa
    17521 cagctgctca gagtatcctt ccctgtggat attctggcac atgaagcgga ttatgagatc
    17581 cagttcggca atgtaaagcg gccgacacac aagaacacat cctgggatcg tgcaagattc
    17641 gaggtatgcg cacacaaatg ggcagatctt tccgagcccg gttatggtgt ggcactgatg
    17701 aatgactgca aatacggtta tgatgtccat gagggtgtga tgggactgac attgatcaag
    17761 tcaggtattt tccccaatcc cgatgcagat cagggacagc atgaatttac ctacgcattg
    17821 ttccctcatc agggagattt ccgcaagggc agtgtggtaa gagaagctta cgatctgaac
    17881 tgcccgatgg catgggagaa ggtacagggt atctatgaag acagcttctc aatgctgcag
    17941 atagcagagg aaaatgtgat ggcagatact gtgaaagcag cagaggacgg caatggcatc
    18001 atcgtacgta tctatgagac ttggggaatg cgtaccaaag tgcatgtgaa cttcccactt
    18061 gctccggatg cacaggtaac agtctgtgac tgcatggaga atttttccgg ggtggagaat
    18121 gcggatatga cccgtgcaca ggacggatgg agctttacta tgaagcctta tgaaatcaag
    18181 accctgcgta ttgtgaatga aaaaaagtga gagggttacg aaggagaaaa ttatgatcaa
    18241 tatattagaa tacggtgcag tgggcgatgg acagaccaat gatgcccctg tcattcagaa
    18301 agcaatcgat acgtgtgcag aacagggagg aggcgtagtc ctcttccccg gcggcaggac
    18361 atattcttcc ggatatgtgc tgttgaaatc caatgtggag gtacatctgg agccgggcgc
    18421 tgtctggaaa gccagcagca acatagaaga ttattacccg ttgcgcaaca atggtaaaat
    18481 cacagcccat gaatccggcc tgccttcttt cctgaacagt gaatatgcag gacgcccgtt
    18541 ccatgccttt atctatgcgc tgggacagga aaatgtgacg atctccggac agggtaccat
    18601 tgatggcaat gaatctgtct tttacggaga tgactcgggt tatcatatcg agggttccta
    18661 ttatcccaga gttccgctcc tgctattgga ggatgtaaag catctgacca tcaaggaagt
    18721 ccgcctgacg aactgtgcct tctggacagt gcatctggta ggctgtaagg atgtgctgat
    18781 cgacggtatc cgcctgctga ataatctgaa gatggcgaat tccgatggca tcgatccgga
    18841 tcactgtcag aatgtccgga tcagtaactg tcacattgaa tgtggagatg acgccatcgt
    18901 attgaaaaat tccggggatt ataagaaata cggtccctgt gaaaatatcg tggtcaccaa
    18961 ttgtactctg gtatccacca gtgctgccat caaattcggc acggagggag agtctgactt
    19021 ccggaatatt ctggtggaaa attgctgtat ttccagaagt aaccgtggaa tttccattca
    19081 gatcagagat aatggcaacg tggaaaatgt aatcttttcc aacatcatta tggagaccag
    19141 aagattctct tatgaatggt ggggaagagc ggaggctgtc tgcctgactg cgctggatcg
    19201 caaacccgga gttaaggccg gtaagatccg caatatccgc ttccagaata ttacctgcga
    19261 gggagaaaac ggcattttcc tgctgggcag tgaagacaat tatattgaag atgtgacctt
    19321 tgagggcatt cggctgacac tggataagaa atccaagtgg gagatcaacg gctacgataa
    19381 gagaccctgc cagggagagg gagagatcca cacaaagatt tccggaatct atgcggatta
    19441 tgtgaagaat gtctccttcc atgatattca gatcacgaag aaagaaggaa tcctgccttt
    19501 ttacgaggag gacgtgacac tgggaaatgt agaaaatgta cggatcgatg tagaagagcc
    19561 ggaagatggg agccagaatg gagaagagta atagaaggat cgctctcatc ggatttgcaa
    19621 tcctgatggt cggcctggga atcagtgaca gcctgagagg tattttctcc ggcatttttc
    19681 agacgcattt tgcattgacg gcaaaggggc tgtccacgat catagccgta agctatgcgg
    19741 gaaatctgtg cttcctgctg gccggaggca gaatcatgga taagatcgac cgcaaaaagg
    19801 cttttttggg atttctcgca gtatggatgt gtgcaatgct ggtattcctg cttacagata
    19861 actattatgc attgctggtg ggaatgttcc tggcaatggg agcttctacg ctgttgaata
    19921 cgtccattac actgatcaca ccgttcattt ttgccacatc accggccatg atcgtcaatg
    19981 tattgttctt tacgcaggga atcggtacca gcggaggaca gagtctgatc ggaaatctgg
    20041 ctaacgattt tggcggatgg aaagctgtga caggggtgct ttttgccatc ggcgttctgg
    20101 cgttcctgat cattttaaaa ctgcagatcc cggacagcgg agaagtgaag aaggcagaaa
    20161 aggatcggca gggaaggaaa atgaagggaa actacctttg gattgtcatg gtggtgatct
    20221 ttggtgtata ctttattgca gaacatggaa tgctgaactg gtttgtgatc tacggaacca
    20281 atgcgctggg actggagcag ggaaaggctg ccaattatat ggcgttgttt tttggcggaa
    20341 tgacggtggg aagactggtc ttttctccgt tggttgaccg gctgaaggta ttcccgagca
    20401 ttgccttgtt ttctgtgatt tctgcagtgc tttatgcggc agggatcttc agtggggcac
    20461 tctggatgct gagtctggcc ggactgttct gctcgatcat ttatccgaca ctggttatgg
    20521 ccatcgggag actttatgat ccgtcgcaag tgtctacgat gtccggattg atcatcagtg
    20581 tggcatccct gtttgatatt ggatttaact atatttacgg agacattgct gaaaaaacag
    20641 gatatggcag gagctttctg atcatgccgg cagcaatgat actctttact ctgttgtttg
    20701 tgctggtgtt ccataagcgg atcctgaaca gggaacaggc atagtaaaaa gttgtaatgg
    20761 gaggatgtta gaatgaaatt aggcggaatt gaagccggcg gcacaaagat ggtatgtgcc
    20821 ataggagatg agcagggaaa gattttcgac agggcagtat tccctacgga gacaccggag
    20881 agtacggtgc cgaaaatgat agcatatttt caggataaag gaatcgaggc actggggatc
    20941 ggctgtttcg gcccagtgga tctgaataaa aaatcagcaa cctacggcta tattaccagc
    21001 actccgaagc tggcatggag attttatgat tttgcgggag cgttccggga agcactgaaa
    21061 gtccctgtag gttttgatac agatgtcaat ggagcagtgc ttggcgaggt acgcttcggc
    21121 gcagccaagg ggtgtgagaa tgcactctat atcaccattg gtacgggagt aggtgtaggg
    21181 gcatatatta acggcagact gctgcatggc ctgatgcatc ccgagggcgg acatatcttc
    21241 ctgcgcaagc acccggagga tacctatgaa ggctgctgtc cttatcacgg tgcctgtctg
    21301 gaaggactgg cttccggtcc tgcgattcag ggcaggtatg gcagaaaggg agctgaactg
    21361 gccggaagag aggatgtctg ggaactggaa agttactaca tcggacaggc tgtggcagat
    21421 tacatgctga cttactcgcc ggagaagatc attctctggg gcggtgtgat gcaccaggag
    21481 aaggttttcg acatggtgag acagaatgcg gtggagtttc tgaatggcta tctgccggag
    21541 accagcctgc cgaaggatat gtcacagtat gtggtggctc ctgcactggg cgagaacccc
    21601 ggaatcatcg gtgcaatgtg tctgggaatg gatgcatacc tgatggaatg cgggaagaat
    21661 ctgtgaggag gcttgatgaa gtagcggttg ataatccaga gagggtgaga tcaccagaga
    21721 gttttcggcg atctcgccct cttttttgcg gtcctataag gcaaggatct cgggagaatc
    21781 cggtttcgtc gtgtcggggt tggcaccgcc gagacgtcgg ggactgtaag tggtgtgtgg
    21841 aacgttgctg ggcagttcca agattgcgga gatgaaaacg tgtaacaaag atttgaaatt
    21901 atgtaataaa taatcataat tttgtcaata cacttttggc actaaaagcg aaaagtgccg
    21961 aaaatatttt ggtggatgac aggcatagag tatggcaaca gcaggttgct tttcataaca
    22021 tgaatttatc tatatgatat ggttatggta cgataatgca gaacaaaatg attatacagt
    22081 aacccaaaca gaactttcct ctgaaggggc acagtagcaa aggctgctat tgcgggtagg
    22141 taaggaaagt cggccagaca atctgagagg gtattgtgta agcaataatt agggtatatg
    22201 agaatatgat gagaacccca ttactgatag aaatatcagt aatggggttc ttttttggaa
    22261 aaatattgtg taggggtcat gccgtagtgt tttttaaaaa cccnnnnnnn nnnnnnnnnn
    22321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnntcttttt tggaaaaata ttgtgtaggg gtcatgccgt
    22441 agtgtttttt aaaaacccgg taaaaataag aaacgctttg gaaaccacag gacttgctga
    22501 tgacagagag agaattgccg ccctgttcca cctggtgtat ggcatgacgg aggcgaagct
    22561 tattaatata ttcttttacg gtgagggatt ctgtccgccg gacagcccgg tggacatagc
    22621 tttcggacac accgcaggat tcggcgatat cccggacggt ctgaagcgtt gtatagtttg
    22681 cgttaatata attaatgatc cgctgaaaac cggaggcttt attctcactg acgggaatct
    22741 cattatctaa cggatactga tgacgaaaat cgttgagatc tgtaagaatc tgtgccaata
    22801 acagataggc agtgggagat ttgcagcaag aagctgtatt ccagtcgagg agcttttgtc
    22861 tgtaatcctc gggaatgaaa atgatcggtg ccttgaaaca ggtcaggtag gaagatacta
    22921 cggcaggtaa aaagtatttt tgaagatagg tttttgaaaa atggatgcag atcccagtat
    22981 aatccgtgtc accgacgctt cggtgagaga tattgctctc aaacaggctt gccatgccgg
    23041 cttctacccg gtaggtgatg tcgccgatgg taaccagacg ctccccggag agtagaatat
    23101 agatttcata attggtgtgg tgatgcgaat agggcatgga ccatcctttt tttgtatctt
    23161 cataggatat ttcaaaatct tcatataaaa aagttcttct atctgtcatt tctgattcac
    23221 tccttcacgc atattatatc aaaaaggttc agaataatgc aatagatata aaaaatagtt
    23281 catgatagtg aatttaaaac actaaagtta agctatataa tgattccgtg atcaaggaga
    23341 aaacatcaga aaatttacta tggatctgct atgggatttt ctgtatggaa aagggtctgt
    23401 gggatggagc ttatatgaga atattgttga atggaaaatg gcatgtagta ctggaggacg
    23461 gaaccacggg acagatggat ctgccgggga cgttggatga gaacgggatc ggacatagag
    23521 atgtgggggc gaatcagtgg catccggatg cggtactggg gaatgcggcg ggagagatag
    23581 acaaagatgc accgatcgct acgagattta ccaggcggca tacctatgag ggagaagcca
    23641 ggatcagtcg taagataacg gtaccggatt acggaaccga caggctgttc gtactggcgg
    23701 agcgggcacg ggcactccgg ctgctagtgg atggagaagc gtgttcggtg ttccggcagg
    23761 ggacactaag tacaccttat atttttgaac tcaccggggc ggcaccggga gaacatgaat
    23821 ttacgtttct ttcggataac agctatccgg gaatgcccaa agctgccatc tgttattctt
    23881 ccgcagcgac ggatgagaca cagacaaact ggaacgggat acttggcgaa tgcagcatgt
    23941 ataccagacc gcagaatttt atagacagcc tccgggtata tccgagagcg gtgaaaaagg
    24001 aagagaaaaa taaagcaggc ggatatgtgc tggatgtatg tgtggaactg gcacctggcg
    24061 caaagaaagt atataaagat gcgaaaatca tattacagtc agaggctctg gcggcaggag
    24121 agttagaaga cacacagaca ttgacggaaa ttatcagcta ttccggggag ggattagcgg
    24181 aagccggaac agataaagag gagaatccga aaacaatgga aatctggttc cgtgatctgc
    24241 ctctgcggga gaatgtgaaa ctgtgggacg aagatgaggg caatctctat gagatggctg
    24301 taaccctgga caacggtatg tcagcagaag ataaaggcgg tagcactgct gagtgcagga
    24361 cccgtttcgg catccgctct tttggtgata acggcagcgg cagactggca ctgaacggta
    24421 gggcaatttt tctgcggggt gaggcaaatt gcgcagaata cccggagacc gggcatccac
    24481 ccatgacaat tccggaatgg aaagagatgt tattaaaata tcgcagttat ggaatcaact
    24541 ttgtaagatt ccattcccac tgtgagccgg aagctgcgtt tgcggcagcg gatgaactgg
    24601 gaatgcttct gcagccggag ctgtctcact gggatccgaa ggatgccttt ggaacggagg
    24661 aaagctatcg gtattaccgg gcagaacttg tggacctatt gaaaacctat gccaatcatc
    24721 cctcttttgt aatg
//
LOCUS       DAWEJW010000031        24659 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_832_length_24659_cov_63.593440, whole genome shotgun sequence.
ACCESSION   DAWEJW010000031 DAWEJW010000000
VERSION     DAWEJW010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 24659)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24659)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24659
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_832_length_24659_cov_63.593440"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7903..8002
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5654 a         6811 c         5122 g         6972 t
ORIGIN      
        1 aaaaacagac cgcgcatcct tattagatgc gcggtctgtt ctatacgata atcccctttt
       61 gcttttgata cctttggtat catttcataa attatataaa acatctcatg aattgtcaat
      121 agtactttca ttttttgtcg tatttttcaa aaattataaa agtactatcg tctcccgcca
      181 cacttcgcac aaactctcca gcctccatgc cgcattggcc gggctggtgg agggcagcac
      241 cgtggcttct atgccggtca ggggcagctg atattttcga taaagctctc ctgctttgtt
      301 accgttggca tagatccgcg tgatctgtga ttccgccaga atccttcgaa tatccgtcgg
      361 ctccacattg cggatgctac tgtcgctgga tcccttaata tcgcagctct ggattacatc
      421 ccagacggca atgtgatgag ccagtagcat tttctttttc tcctctatgg tctctggcac
      481 cggttccccg cacagatctg ccatcacttt ccagaaacgg ttctgcttat ggccgtaata
      541 aaaatggttc tcccgggatt tcaccgaggg caatgttccc aggatcagca ccagcgaatc
      601 cgcatcgtac accggtgcaa acgtgtgctc aatatgttca tatcctgtca tgctgttttc
      661 cttcttcctt atggctgtcc ctgtctttca gcagatatcg ggccgcagga atttccacga
      721 tcagagtaac cgctaccgcc accgccgcag acaccacgat aatcagtatc tgatatttcc
      781 actgccatac acggtcacct gtcatatttg gcggcgtatc cccgctccaa taaggaattc
      841 ttttttcttt cagccagacg gcgatcatct gatgccaaag atacagatta taggaaaatt
      901 tgcaaaaaag tgatgccacc cggttggaat atagtttctg ataatattct cccgcacagg
      961 ccgtactgaa aaggaacaat gcaaacagaa acgactgcac cagccggctc tgcagctgaa
     1021 ctgcctgcag attcacatcc ccgtcgtatt tatgaatcat cccgttcatc agaaaaatac
     1081 acaaaacaga gatcactgtt cctgccagcc gcgagtattt attttctgtc acatatttct
     1141 tccacatgac atacaggatg cagcaaagca tgccattggc atagattccc ataaacgtca
     1201 gaatattatt tccataattt ctctcttttc ccgcaaaaca gttcagaatt accgcagtac
     1261 ttgtaacgga aatcatcaac atcacactat aagtggcaaa ggcccacttg cggaacaatc
     1321 ttgcgatcca cggcagcagc agataataga tcacttccat ctgtaccgtc cacaacacgc
     1381 cgttaaaaga ggtggatata taagtgtccg accagtttgg tgcaacgcaa aatacatgtg
     1441 tcacaatatc tttgcacaat gcccccacat tccgatattt atgcccaggt aaaatataaa
     1501 aaaacagatc gatcgtcagc ataaaatagt aagaaggcaa tattcttgct gccctctttc
     1561 tgtaaaattt cttcgtatcc ggccagtcct ctcccagaac aatagatctt gcataaggat
     1621 aaaaattgca gaatgcgctg agcaggatca tcatatccac aaaaacgaat ccatatctga
     1681 cccatccagc aaggttgatc tccgtgagtc caatatattt tgtgtactct gttggaatcc
     1741 gaagatacgg tgtcagccat gtctgctgcc agaaatgaaa ccacactacc atcccgatgg
     1801 ctatggcacg gattccatcc agcacaccga ttctgttttc tattctgttt ttttcatcca
     1861 tgctgtattc ccgccgtttc tgattttctg ctgcttccta tctgtcatgt tccagtctcc
     1921 atctgataca tctggtgaga taatccacgt aatccgcatt cttacacatt tccttgccgg
     1981 gagtatcgga gatctttgcc acatccatgc cgttgcaggc agtggtcttc atgacaatat
     2041 tcaacggttc ctcgcaggta tcattggcaa tataggttcc gataccgaaa gccaccgtca
     2101 ccctgtcccg gaatgcctta cgcagttcat ctgcccgtgc aaagtccagg ccgtcactga
     2161 acagcagggt cttgcgcttc ggatcaattc ccagggattc ataatgagat atcagcttct
     2221 ccccccatgc aaagggatcc ccactgtcat gccgcacgcc ggaaaacaga gtggaatagg
     2281 tcagctggaa atctttcagg aaacaatccg tggtgatcgt atccgtcaaa gcaattccgt
     2341 tcaaaatgcc atactctttc acccaggcat ccagggcata ccagttggaa tatgccggat
     2401 tgtgcttgtg attgccctgc ccggtacaca tgatccattc atgggccata gtgcctaccg
     2461 gtgtcacgtt ccatttcttt gccagataga cattggaggt accaatgcag gcggagtcct
     2521 tataatcctt attgtgaccc accagcttct gtactgccag ttcctgtgcc tctgcagaga
     2581 gacggcgtct cagaccgaat tcactgaaat ttccaaggca atacgttccg tccaccagct
     2641 tctgtagctt gtctgccaga cgctcccgga aggaggcata cagcttctca tagtcataat
     2701 tcatacggaa aaatacttca ttaacaatag ccagcgtcgg gatctcatac atagaagtat
     2761 tcagccagga gcccttcgtc tcgatagtca ggccacagtc tgcctccgtg ccgatctcaa
     2821 aatcctcata tctgggctgc cacaaacgta aaaaatccac ataggaaccc ttgatccatt
     2881 ttacgttctc cagataagcc agttcctctt ccgtaaaacg taagtcacaa taggcccgga
     2941 tctgcgcctt gatttcttct accatttcag atgtgaaaaa tacatctgta ttcctgcatt
     3001 tgaaggtcca ggtagtctta taagcgctga actggtggta gatacactgt cccatagaga
     3061 acttgtacat atcattttcc agtaagctgt tgatgatctg tggtaattgc atgattttat
     3121 cctctttctc tcttagattc acaactgtgt aaatatgata tgttctctaa atcacatccg
     3181 cctggatcgg tcccatagct gcgatggccg tctggtgcgt ctggggtgtc acacaggcac
     3241 aggccttctc aatgacacac actctcgctt ccggtacaaa tgcctttgcc atgaccacat
     3301 tgcttaacac acagattccg gtgcagaatc cggtaaaatc cacttccatg ggatattcac
     3361 cctctgctgc ctctcctgct gccgtcacct cttcccagat tgccttcagc gtattcccaa
     3421 aatccagact gccaaaagta ggctttttca ccgtaaggat ctgttccggc gcataatgct
     3481 cctgtaccgc cttcatgata tccggatgga tcttatatcc ttctgtgcct tcctgtccgt
     3541 gcagaacggg cagacgcttt ccttcctggg tgtgcagata atccgccgta tgggtatcct
     3601 ttgtaaagat caccttgtag tattccccgc tttctgctgc cgcaacgata tactttaccg
     3661 ccgcctcgca ctccttgttt cccagcgccc cggtgatgaa ttcgttctgt gcatccgtga
     3721 tcactaatac tttctttgtc atgtccctct cctctactcg tttttctata atgatgccgg
     3781 ggcaaaagcc cccgactttg cacaaaaagc aggcaaccgc atcctgcgac tacctgcttc
     3841 tattacttat agaaataatt tcttcacttt ttctgcatca aatacaactt cctttacccc
     3901 gtccacttcg atctgataca gggcattggc tgccagatgc tccgccgcct gctcaaggtc
     3961 acggattcca ctggtgttgg aatagatctc caccaccgca tccagtccct cttcggtgag
     4021 tgtgcattcg tctgcctgca gtccaatcct tttcagcacc ttcggcaatg caaatctgga
     4081 gaaaatgatc ttcttctcct ccggtgtgta atccgggatg tcgatcactg caaaacggga
     4141 cattaacgga gcactgatct gctccttgtc attggcagtg gcgatggggt agacaccaaa
     4201 ggtgggaacg gtacattcca tatagttgtc cgtgaatccc agattgtcaa gcagtgtcag
     4261 aagcacatcc gccggattgc cgttaccctt gccgtttgcc gccttgtcca actcattgat
     4321 gatgaatacc agattagact ctcccgccat ggaaaaagcc tccatgataa tgcccggctt
     4381 ggcattggca tagattctgg agcttccggt cagctgctcc ggatcgttaa tggaactcat
     4441 gtctaatgtt gtccagggca atttcaggat cctcgccacc gcataagcga tctgggattt
     4501 accggttccg gccggaccca ccagaagcag tccgtaagcc ggtaaggtgt gagtacggtt
     4561 aatctggata atggtctcca tgatccgctg cttgactctc tccatgccgt acaattcctc
     4621 gtccaggatc cgtctggctt ccatcggatc aatggcttcg aagtaattgc ccttccactg
     4681 aatgttcatc atgatggaca gcgctctctg ggcatgtctt ctctcttccg gagacacctc
     4741 atgggatctg gccaccgcca gatttcttct ggcccacagg cggatgtttt ccggcatggt
     4801 ccttccagca catgtcatga aatcggagat actctgaagg ctggtaagct tcatatcatc
     4861 gccgtcttcc tcttcgtcct ccccctgttc ctcttctgcg ggagcactgc tggcgaacag
     4921 tctttccatc atgaactgta aaaatccatc ttctgccgaa agcttggttc cgccgccaaa
     4981 tgcagtctct cggatcttgt ataccgcata gccttcttcc cggttttccc cggaaagccg
     5041 gacattgatg tgattttctc ctaaccatct gacgaggctt acgaatcttg catcgatctg
     5101 taaaatatcg gcataggcac tgctgccctc ttccctgaat tcgaaatttg tgcgtttgtt
     5161 ctgattgccg aagacaccga tagcatggtg cttccacggt tcgctgctgt tgtccaggat
     5221 caggatcggc ttttcgggac ttgccgcagc ctcattggaa cggaacatgg tataggtgca
     5281 tacgggatcc tgcttcttct cattttccgg aatactgctg aaatcaaata ccggcattgt
     5341 cagcctcctt ttttctcgcc tgaatcttct cctcatcgat cagcttgaac tgctttacca
     5401 gattggtcat attctcggac tgagccagca gttcctcact ggtagcggat aattcttctg
     5461 ctgccgcaga gttggactgt acgatctcgg agatctggtt cacaccgctc tccgcctgat
     5521 tcatggcatc tgactgagca gctgcaatct ggttcagttc ctcagaatcc tgtgcgatct
     5581 tgcggacgcc ttccacgata acgctcaacg tctgggacgc atgatccaca gcttcattgc
     5641 cggcatggat ctcctccagt gtaccgccga tcagctctct ggtattgaca gcggactgcg
     5701 cactctgctc tgccagctta ccaatctggt ctgccaccac agcaaaacct cttccggcct
     5761 cccccgctct ggctgcctca atggacgcat ttaaggacag caggttggtt tgactggcaa
     5821 tatcctcgat ctcaccgatg atgttgctga tcttctcgga agtctcgttg atctgttcca
     5881 tgatctccat cagatgattc atctcggtac ggccttcatc tgccttagca gagtattcct
     5941 gtgcctgcct gctggaattc ttggtatgct ctgcagtatt gtctaccata gaggtaatat
     6001 tagtaatggt tgcctgtagc tgctgtacgg ctccggcctg ctcagtagcg ccctctgcca
     6061 gagactgtgc ggcatctgcc aggcttccgg cacccatgga tacctggccg gatacggaat
     6121 ttacattgta caatgcttca ttcatcttat atttgatatc tctcagggct tccttcaatt
     6181 catccagatc ccctgcatag agatcggggt aaagaaattt taccgtccag tcaccatttg
     6241 ccatggcatt cagagcctgc ttgatatcct caatgatcat tcgcagattt tcagccattg
     6301 caatggcggc acgattcatc tctgccactt cgtcctctgt ctttacttcc gggaactctg
     6361 aagacagatc acctgcagca aacccatcca gtctgtctgc cagcgctttc agcggtccga
     6421 cgattccttt ggctattctt tttcccgctt tttcagatcc cgcaaagcct actccgatga
     6481 tcagaaggat tacaataaaa ataaccacac ggaaaacatt cagtttggct tccatggcat
     6541 ttccgttcgt tacactgctg tccaacaggc tcgtcatatc tgcatagacc tgctggaaca
     6601 cgggatccat ctgttcgcct gccatctgct gtgcctgcat ccgcttagca gaatcggaag
     6661 ctgttcccag ttccagtatc tgtgcctcga tctgctgata agaagctacg ccgtccttga
     6721 tctgctggta ggtagctctt ccctcatccg tgatcatgtc atcttccagg gcactgatat
     6781 aggtatccaa ctgttccgcc gtcaggtcat gggcattttt tgctgtagcg atcagtgtat
     6841 cgtcatcata gccgatggcg gcacgggtct cactccgcat ctccgcgata acgatcatcg
     6901 ccttaccgat gtcaccctgc gcgaaaccgt aatgcttcaa ggcgtaggaa tactgataac
     6961 tcacgattcc catggccaca caggcgatga cacccgtcat gctcagcagt atcgtggtct
     7021 tacgaaatac tttcaatagg cgcttctcaa tggagagctt gtttaaactg ttaaatctgt
     7081 ccgttctgtc cattccaatg ttccatcctc tctgtaaact gtgtgtaaaa atctatcgtc
     7141 ccctcatacg gcaataaata aaacagacgc agcagcagga gattttcttc cctctgtttc
     7201 ccttcgtctt ctcctgtcag tctttcttcc atggcatgta acaggtcatg ccattgtaag
     7261 gtaaattgct gattgcgggt cagatccgga gtatcgatcc atttactgac ttttatcttg
     7321 ctgcggttct cctttgcaca ttccccggtc tgaagaaaat aggaaaatcc tccattttca
     7381 taatatcttc ccagcggaaa cagtctgcag attccaggcc ggtacggatg gatgctgcat
     7441 cttccttcct catccagata agcgcaggct tcctctgtcc cctgcatttt cagattcggt
     7501 aagatcacac cgtccaccac attgagctcc accgatgccg ctaatagctc ctgcatgctt
     7561 ttccccattc ctgtagtcat ccggtacaca tcatagggat ccaggataat ggaattgccc
     7621 atgccgtgac aacaccggga acagccattg caaccaccgc aatccgcttt taccatatca
     7681 tttgctttgt ataatcggcc gtcggatatc tccgcaaggc ttacatttcg cttcatatct
     7741 acaccatcag cctctctgtg ccttgcgttc catgatctcc ttcaggctct cggtgtaagg
     7801 agctctcgcc accccgtatt ccgtgacaat tccggcgatc agttcatggt ccgtcacatc
     7861 gaatgccgga ttgtagacct ttacaccctc cggagccatg cgnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnttggacat gacagaagcg gacatattgt cacagatcag
     8041 tgtcacatcc acgcctgcgg aatgcagttc aaatgctgtc agtctggctc cctgcaaaag
     8101 tggtctggtc tcatccgcaa atacatggaa gttgtaacct ctctcctgtc ccagataaat
     8161 cggtgcggtt gcggtaccgt attttacggt tgcaagttgt cctgcattac agtgggtcag
     8221 aataccatct cccggtttta ccagttccag tccaaattct ccgatttttc gacatacttc
     8281 cacatcttcc gcccgaatct gtagtgcttc gtcatgcagg atttccttca cttttgcgac
     8341 agtcttgtgc tcttctctgc cagcctttac agccacagca tccatgcgct tcagcgccca
     8401 ggacagatta actgcggtgg gtctggaaga atccagatat tccttatatt cggtaaattt
     8461 ctcataaaac ttttcaaaat cttcggtctc gatgcggttt gccagcagat aaatacccag
     8521 tgccgccgct acgccgatgg ccggagcccc tctgactttc aacagataaa tggcatccca
     8581 gatttcctgt gccgtatgta aagcgatcag ttcgatcctg ccgggtaata aggtctggtc
     8641 tatgatgatc accgcatcct gtgcttcatc cagtgccacg gtgtcgtaat cgagaattgt
     8701 tttttctttt tccatagata ctcctttata tatgttttcg taatcgtcag atccttacgg
     8761 tatcttcccg gttaattttc atgccggata atatcggcaa attctgcttt cgctgccttg
     8821 gccagatcct ccggtgtcag acggatctga gatccgatct tgccgccact gacatagatc
     8881 tgctcctgtg tctttgcgct ttcatcgatt ctggtcacgt actgcttttt cattccaatg
     8941 gcagtacagc cgccgcggat ataaccggta atggcgttaa tgtcctttac gtggatcatt
     9001 tccacgctct tttcccctac cgatttcgct gcctttttca gatccagctc cgccgcaata
     9061 gggataacga atacaaaata attcttgctg ttgctcacgg tgaccaacgt cttatagacc
     9121 tgttcatagg gcagggataa tttgtcagct atctgaagac cgtccacgaa ttcctcacac
     9181 tcgtaggtgt gatgggtata aggaatcttc aaagattcca ggatccgcat tgcgttggtc
     9241 tttacttctt tttgttttgc cataaaacta ctcacacctt tcaacaacct gcaaactttg
     9301 agccgaaggc acaaatttgc aggtgaaaaa tttatttttc accttgttac ccttggtatc
     9361 attttatttt actattttgc ttccctcagc gcaagcatac aaggcaagaa acttgcacaa
     9421 gttgcatcag ttttttgcag aaaagaactc cgcccgactc ccggacggac cggggaatca
     9481 gacggagttc ttatatgtta ttaatccgtt gttacgaatt agtttgctgc cttctcagcc
     9541 tttgcagcct tcagctgctc ggtcagagcg ggaactacct tgttcaggtc gcctacgata
     9601 ccgtagtcag ccacatcgaa gataggcgcg gtctcatcct tgttgatggc aacaatgaga
     9661 tcagactctt ccatacctgc tacatgctga atagcaccgg agataccgat tgcaaagtaa
     9721 atctggggac gaacggtctt accggtctgt ccaacctgca gatccttggg cttccagccg
     9781 ttatctacaa ctgcacggga gcaggatacg gtaccgccca gaacctctgc cagatcctcc
     9841 aggatcttga agttctctgc gctgccgaca ccacggccgc cggataccaa gatcttggca
     9901 tccatgatgt caacggtatc ggatacagcc ttaacgactt ccttgatggt tacatatttg
     9961 ttgttcggag taaatccggg attgtactct tcgatcacag cctttgcacc tgcgatagga
    10021 gcgatcttct gcataacgcc gggacgaaca gttgccatct gaggtctgtt gtcaggacat
    10081 gcgatggtag cgatggtgtt accaccgaat gcaggacggg tcatcagcag ctgattgtgc
    10141 ttctgctcct gtccggggat agcaacgaga gggaaatcac cgatttccag tacggtacag
    10201 tctgcggtca gaccggtctg aactcttgcg gaaacggtag ggccgaggtc acgtccgata
    10261 gcggttgcac ctaccagaac gatctcaggc ttatatttct caataacaga tgccagtgca
    10321 tgtgcataag gctcggttct gtaagtctcc agttcgggat cgtccacaag gattacctta
    10381 tctgcgccat actcagccag cttatctgcc agattcttta cattataacc gatcagaacc
    10441 gcggttacct cggtctccag ctttgcagcc agctctttac ctttgccgag cagctccaat
    10501 gcgataccgc tgatctcatt gtctacctgc tgggcgaaca catatacacc cttgtattct
    10561 tcaatattat tggttaccat ggtttgcctc ctatattaga tgatgtgctt ttctttgaat
    10621 ttagccacaa tgtaagctgc tccgtcttca ggtgaatcgg gaacgatctt ctcacctgcg
    10681 cccttacgaa ccttatcgga tgcctttgca atcttggtag gagatccctt caggccaagg
    10741 ttgctgtcat caacatcttt cagatcagct ctgccccagg tagtgatctc tgccttgcat
    10801 gcatcgaaga ttccgccggg agtcatataa cgaggcttat tcagctcgga cagtgcagtg
    10861 atcaggcagg gcatctgagc ctctactaca tgatatctgt catcatactg tctctgaacg
    10921 atcaccttgt ctccctcaac cttgatgtcc tgtgcataag agattacggg aagtcccaga
    10981 tgctcagcga tctgaggacc aacctgtgcg gtatcaccat caattgcctg acgaccggta
    11041 atgatcagat catagtcaag gtttctcagt gcacctgcaa tggtagtgga agtagcccag
    11101 gtatcagcac cacccagtac tctgtcggta accagaattg ctttgtcagc gcccatagcc
    11161 agcgcctcac gaagagcatc tgctgccttg ggaagaccca tggtcagtac ggtaacttct
    11221 gcgccgaact cgtccttcag cttcagtgca gcttccagac cagccttatc atcgggattc
    11281 atgatagcaa gcattgcgcc tctgtcaaga gttccgtcag gattgaattt aacaccgccc
    11341 ttggtatcgg gcacctgctt aatacaaaca acaatcttca ttgtattttc tccttacttt
    11401 tctaataaaa tttccgacaa atgcctgctt gcttatttca gcagatctgc ggagataacc
    11461 attctctgaa cttcggaagt accctcgtag atctcggtga tctttgcatc acgcatcata
    11521 cgttcaactt cgtactctct gatgtaaccg taaccaccgt gcagctgaac agccttggta
    11581 gtaacttcca tagcaacctc agcagcatac agcttagcca tagcagcttc tacagagtaa
    11641 gagatcttag gattggtctt gtactcgtcc ttcttgcgtg cagccttgta taccagcatc
    11701 ttggcagctt ccaccttggt agccatatca gccaactgga actgtgtatt ctggaatccg
    11761 gaaatggact ttccgaactg ctttctttcc ttggtgtaag caacggttct ctccagagcg
    11821 ccttctgcaa tacccagagc ctgggaagcg ataccgatac gtccgccgtc cagagtgtgc
    11881 attgcgatct tgaatccctg tccctgctta tcaagcatat tctccttggg gatacggcag
    11941 tcggtgaaga tcagctcgta agtggaagaa cccttgatac ccatcttctt ctctttggta
    12001 ccgaaggtaa atccgggagt acctttctca acgataaagg aagaaatctc cttcatcttt
    12061 ctgcctctct tctctacgat accggtaact gcgataacaa tatatacatc agcttcttta
    12121 ccgttggtga tgaagcactt ggatccgttg atgacccact cgtcaccatc taatacagcc
    12181 ttagtctgct gtccctgagc atcggtacct gcaccgggct cggtcagacc gaaagcaccg
    12241 atcttacggc cgcttgccag atcgggaaca tatttcttct tctgatcctc agtaccgaag
    12301 gtcatgatag gatcgataca cagagaggta tgtgcggata cgataacgcc ggtggttgca
    12361 caaaccttgg aaagctcctc aacacacata gcgtaggtca gggtatcaca gccctgtccg
    12421 ccgtactcct tgggaacggg aattcccatg aagccgtact tagccatctt ctcaacggtc
    12481 tctctgggga aggtttcggt ctcgtcaact tcctgcgcaa gaggttttac ttcgttctcg
    12541 gcgaactctt tgaacagctg tctcgccatc tcatgctctt tggataatgt aaaatccatg
    12601 atgttaatcc tccattgtta tttatacttg gttgtaatta tgaacttaga ctgtccagag
    12661 ccgtatccgg ctccgaacag ttattttacc ttttgcctac tgtgcatcca caggagtctt
    12721 ggtgcggtct gcattgtata catagaagcc cttgccggtc ttcacaccaa ggttaccgcc
    12781 gcgaaccatc ttacggatca gagggcatgc acgatacttg ctgtcaccgg tctcgttgta
    12841 cagaacatcc atgatagcaa ggcagatatc cagtcctacg aaatcgccca gttccagagg
    12901 tcccatggga tgattagcgc ccagcttcat agctgcgtcg atacctgcga tatcggatac
    12961 accttccatc ttgatgaaag ctgcttcatt gatcataggg atcaggatac ggttaacaac
    13021 aaagcctgct gcctcgttta cctgaacggg ggtcttgccg atctcttcag atatcttctt
    13081 gatggtgtct acagtctctg cgggagtatt cacacctgca attacctcaa ccagcttcat
    13141 acggtctgcg ggattgaaga agtgcatacc gatcagagga cgggtcagtc cgttgccgat
    13201 ctcggtaatg gaaaggctgg aagtgttgga agcaaataca cactcgggct tgcagatctt
    13261 gtccagctca ccgaaggtgg tcttcttaac ttccatattc tcaaatgcag cttctacgat
    13321 cagatcgcag tctgcacaca gatcttcctt caggcccgga gtgatcttag ctacgatagc
    13381 gtcaacagcc tcctgagtca tctttccttt ttctaccagt cttgcatagc cttttacgat
    13441 cttggccttg ccgccgtcag cccactcctg cttgatatcg caaagagcta cttcatatcc
    13501 gtctacctgg gcaaatgcct ttgcaatgcc ctgtcccata gtacctgcac cgataatacc
    13561 tactttcatg gtaaatcctc ctgattttat acttttatat tcgtaatgat tagcagttct
    13621 taaaaggctc ttttaccttc tccttgttct tgtcaaggaa gaaagccata ccatacttct
    13681 gatcttctgt ctcgaagcag tcaccgaaca acttctcttc gatcactact gcctgatcca
    13741 tatctgcatc caggccttcg ttaatagcct tcttgcagtt gcggactgcg atgggagcat
    13801 tctttgcaat ggtggctgcc atcttctgtg ctgctgccag cattacttcc tgtgcactct
    13861 ttaccacatt gccgtctgca tctacttctg cgctgtaaac ttcgtttacc agaccgattc
    13921 tgtaagcgtc tgctgccttg atattgcgtg cggtgtagat catctgtttt gccatgcctg
    13981 cacctaccag acgagccagt ctctgggttc cgccgaagcc gggagtgatt cccagaccta
    14041 cctcgggctg tccgaagact gcattgtcgg aacagattct gatgtcacag ctcatggaga
    14101 tctcacatcc accacccaga gcaaagccgt tgacagctgc gatcacggga atggggaagg
    14161 tctcgatctt gcggaataca tcgttaccct tcttaccgaa tgcctcgccc tctgccttgg
    14221 tcaggctgct catctctccg atatctgcac ctgccacgaa ggacttactg cctgcaccgg
    14281 tgatcaccag acaacgaacc gttgccagat ctactgcatc caatgtagcg ttcagctcat
    14341 ccagtacctg agaattcaga gcgttcagtg ctttctcacg gttgatggta atggttgcca
    14401 cattgccatt ctgctcatac aaaatgtaat ccatgttttt acctcatttc acatttcaat
    14461 cagtattttt aaaaccacac taaggcgata gagattctac cgccccagcg aattgttatt
    14521 ccattagtcc tcgatcttaa cgatggtgga gcatcccata ccaccaccga tgcacagagt
    14581 agccagaccg gtcttggcac ctcttgcctg catctcatgc agcagagtta ccagaatacg
    14641 gcatccggaa gctcctacgg gatggcccag agcgatagca ccgccgttgg ggttcagctg
    14701 cttatctaca tcgatgccca gttccttacc aactgctacg gactgtgctg cgaatgcttc
    14761 gttagcctcg atgatatcga agtcttcgat cttcataccg gtcttagcca ttaccttctt
    14821 ggtagaagct acagggccga tacccataac ctcgggacga acaccagcca gagcgcctgc
    14881 tacgaaagta gccataggct taacgcccag ctccttagcc ttctcttcgc tcataacaac
    14941 gatagctgct gcaccgtcgt tgataccgga agcgttacct gcggtaacga atccaccggg
    15001 attgatggca cgaagcttag ccagaccttc gatggtagaa ccatgacgag ggccttcatc
    15061 ggtatcgaat acgatggtct cttttttctt cttaacttct acgggaacga tctctgcctt
    15121 gaaggcaccg ttcttctgag cttcttcagc ctttaactgg ctctttaatg cgaactcatc
    15181 cagttcttca cgggtaagtc cccactcttc gcagatgttg tctgcggtct tgatcatatg
    15241 ataatcattg aaagcatccc aaagtgcatc tttgatcatg gtatcaacca tggttgcgtt
    15301 acccatacga tatccgtaac gtccctgagg aattgcgtaa ggagccatgg acatgttctc
    15361 cataccacct gctactacga tatctgcatc accggccatg atcatctgtg ctgcctggtt
    15421 cacacagttc aggccggaac cacataccac gttcatggta actgcgggaa cttcgatagg
    15481 aagtccggct ttgatagctg cctgacgtgc tacgttctgt ccctggccag cctggattac
    15541 acatcccata tatacctggt caactgcttc gggagcaacg cctgctctct tcagagcctc
    15601 cttgataacg attgcgccca gctccggtgc aggagttgtg cttaaggttc cgcccatggt
    15661 accgattgcg gtacggcaag cgcctgctaa tacaactttc tttgccattt gtttgtcctc
    15721 cactttttgt tttcgtttgg tttgctttct gaaatgtaga ttgttatttt tttatcattt
    15781 cactgtcccc attttaacat agcgaaatct gttttgcaat gcttttgtca aaaaaataac
    15841 aaaataacaa atgaaatttt tagaaatcag aattcccgga tttttgtagt gcggatccac
    15901 tctcttgttc cctcgacttt ctgcacttcc tgcgtgtgtt ttgccagaaa acaggtcagc
    15961 ggatagacat cgatccagat ctcgttgtca ctctctttct gttcttcatc aaagaccagt
    16021 tccaatcccc agtgcagatg ggttacctct atattattta cattttctgt ggtactgtaa
    16081 cctgtatgtc ccatataacc gatcacatcc cccgccgtca ccacgctgcc ttcttccagt
    16141 ccttccgcat aggggtagtt ctgccgcaga tgtgcatagt aatagtagcg cttattatca
    16201 aagctgcgga taccaatgcg ccagccgccg tactggttcc agcccagggc ttccacagtg
    16261 ccggactcga cagcaatgat aggtacaccg acgagcccca tcatgtcatg gcccagatgc
    16321 cttcgtttat atccgtagct tcttcccgcc ccgaaatcat cataatgagt ataatcaaat
    16381 cctctcgcaa tggggcaata accttttaat ccgtattttt tctgccaggt gctctgtccg
    16441 tcttctccaa tcacctctgc ctcgtattct cccaccagtc ctcccagtgc cgcgctgtaa
    16501 gcttccagat aatacggata gtatttcata tcttttgtga gagtttctat ctctgcctct
    16561 ccctcagcca gcttctccga cgtctcgttc aggatcttca gcgccttttt gtcaaattcc
    16621 cctccggttc tggctgcagt atatgcaagc agcgggatcc actccacatg atgttccgtg
    16681 tcaaacgtat ccacatccca gtcatatgcc tggcagagcg cttcatagga tacggtaaag
    16741 tccacccatt tgatataatc ctttcccgca gccgaggttg cggtgaccac cgcctgttcc
    16801 atgctttctg tccgtcctgt ctcccggagc agaaatgctc ccatcattat aaatagaagc
    16861 atcacctctg cccggatcca tgcccgcatt ctcttttctc tgtccattcc ctgtcctgct
    16921 ctcagttcct ccttacaggt tatgtttctg atttttccgg tattcagaca attcgaaaat
    16981 ctccggggca tatcatttcc catacaacaa aacagcctgc cggaaactcc ggcaggctgt
    17041 aaatttgtct ttatttaatt ttctgtccgg ttttatactt ccggctcgat cagtccgtag
    17101 gtatcgccct ttctcttgta cactacattt acctgatctg tctctgcgtt gcagaatacg
    17161 aagaagttgt gtcccaacag ttccatctga atgcaggcat cctcgggata catgggcttg
    17221 atatcgaact tcttggtacg aacgatcttg atctcgtcat cgtctgtgta atcattctct
    17281 acataggctt tgctgaaaga agctgccgcc tgctgtttgt caacgatctt gttcttatat
    17341 tttttcaact gtctctcaat gatctcttcc accagatcaa tggatacata catatcgttg
    17401 cttacctgct ccgaacggat aatgcttccc tttaccggaa tggtcacctc aattttctga
    17461 cgttcttttt caacgcttaa tgttacatgt acttctgtgt cgggattgaa atacttgtcc
    17521 agcttgccga tcttttcctc cactgctgct cttaatcctg gtgtaacttc gatgtttctg
    17581 ccaacaataa taaacttcat gctgatcatc ccttccaagt atttatttac aaggttttat
    17641 gtagcatccc ttgtatttat tgtatagtca ttatagcaca atagcacagc tttttgcaac
    17701 aatttaagaa gttttttact tgtttttgac attctgtatg tcaaccctaa aatcgacaaa
    17761 tcctgtcatt ttattatttt tattacatgt tcacaaaaca tttgttcctg ttttttcgta
    17821 aatttatccc ccttgcagtg ccggtttttc tgtggataat gtgtataact tggtgtataa
    17881 gtaactttta tcctattttc tggcattttg gatgtggata acttattttg tatttaaatt
    17941 tcttttctgt cgtttttacg ttcgtgtcac tttttttgta tactttgtat agttccgttt
    18001 tgtcaatccc catttcatcg cgtaacaata ttttgacaaa ttgcacaatt cgttatacaa
    18061 cttatagttt tttgccttat cggcacttaa gttccactgt ccccacagca tcctgtccac
    18121 atggaaaata aaccgaccac atgacacggt gcaatcgagt agtctcgctc atcgtataca
    18181 caaaaaagca ctgtgtgttt ccacacagtg ccttgtactt tctgttatat aatcagaaga
    18241 tccgtcgaac tgccagtacc ttcttgtaat ccgcattaga tacgatgata ccggtcttgg
    18301 cggtagatgc atgaacgatc tggccgttac cgatatacag tgccacatgg ccggagtaac
    18361 agatcaggtc acctgcctgt gcattggcaa gtccgtcaac cgcggaaccc tggctgcggt
    18421 ctgcagaaga ggaatgaggc aggcttacac cgaagttagc atatacactc attacaaatc
    18481 cggaacagtc ggtgccgttg gtcaggctgg atccgccgta tacataaggg ttaccaacga
    18541 actgtaatgc atagtcggca actgcggtac ccatctcgct gtctccgctg gccgcataag
    18601 taactgcggg agcgctgctg gaagagccag aactcttgct gcttgtggct gccttggctg
    18661 ccgctgcggc acgtgctctt tctctctctt ctttctcctt tgccagacgc tttgcttctt
    18721 cctctttgga ttctgcttct acgaattccg tatgcaggga aacaaattct gtagagaccc
    18781 atccaagacc ttcctcggta tctacctgta cccagccatc ggtctcatcg gatactacca
    18841 gatcgtcctc cagaggaacc agacccagca ccgatgcttc cagcgaaggc tcggtacgaa
    18901 ctttcaatgt ggtggtgtta acggttgcca gtcgggttcc taccttcttg gcaagttcta
    18961 ctgcttcgtc gccggtcaca cagtactcgg cttttacata accggttacg gaaccggaag
    19021 aaatctgata ccagccgttc tcttcggaaa tgaagaggcc aacggaatcg tcatacaatt
    19081 taccgacgat ctcaccttcc tcactgggca ggcttcttac atttacataa tcattcacct
    19141 gtgcgattac cagattcttg aaggactcct cctcggatgc ttccggggtg gtatccatct
    19201 gatcgtcacg gatcacagtc tcaataatgc tttcaatctg tttcgtgctg ttggtacctg
    19261 tggaaaggct ctccagcttg ttgccctttg ccagggtcag ctcgataccg ccgctgggca
    19321 gtacagatga agatgctgct gcatttgcag tgatattcat tccggaaaaa gccataaccg
    19381 ctgccatggt gcaaacggta atttttacag atttacgaac cattctatcc tcctgaaact
    19441 cttggagctg tctcaatttc ttaacttatt tctttacatt ttcaaatcat ttgttacgaa
    19501 tttgttacat ccgttaacaa atatatcacg cgaactccca tttgtcaata tagtgaatta
    19561 attgtcaata ttgtcaaaag ttcccatttc atgcgggttt gcggtgtttt tacataaaaa
    19621 gagccaccat ccaggtgact cttttcttta aatattcttc ggttttcttc cgaatttctt
    19681 aatgaatcta ttactaatct gtgtctgtta acgaaaacgg ttgcagtaat ttcccgcgct
    19741 gactgctgca ttggcaccgt ctgccaccgc tgttacgacc tggcgcagag gtttcttacg
    19801 gatatctccg gccacataaa tgccggggcg gccggtctct ccggtctcat ccgcagggat
    19861 ataaccgtct tccccgactg ccaccagttc ccgcagcagt tccgtgttgg gatggatgcc
    19921 tacagcgata aagatgcctg ctgcctccag atctttttcc gcttctgtct tcacattttt
    19981 gatacgcagc ttttccacca tgtcctcgcc acagatctct gtaaccacat ggtcatagag
    20041 aatctccaca ttagggagac tctttaattc ctcctgcaat accatagctc cccgcagact
    20101 atctcttctg tggatcagat acaccttttc gcaggttctt gccagataga tggcatcttc
    20161 cagtgccacg tcaccgccgc cgaccacagc cactgttctg ccccggaaaa aggcaccgtc
    20221 acaggtagca cagtaggata cccccatgcc gttcaattct tcctcgccgg gtactcctaa
    20281 tctggcatgt tccgcaccgg tggcaaagat caccgtacgg gtctccagtt cctggttgcc
    20341 cgcatagacg catttgtaat cgcctcgttc ttccacactg tgtaccgtag cctccaggaa
    20401 ctccgctccc agtttctctg catgctgccg gaacgtcatt cccatatcga atccactgat
    20461 gccgggaagt cccagatagt tatccacctc ataggtattg agaatctgac cgccgctcat
    20521 gggcttttct tctataataa gtagattcag tcccgctctc ttaccataga ctgcagctga
    20581 caaccctgcg ggaccggaac cgataatgat caaatcatac ataattttta ctctcctttt
    20641 gtccagtgta gcagttcccc tatggaaaag caagtccatc tgtgctacac tcgttttaca
    20701 ggtttttagc atccggcact gttttatctc caaagtttgc tgtagaatag cggaacccgt
    20761 ggtagctgat ggactttgcg gatgacttat gccgggctgc cgccgtgaat gaaaaacaat
    20821 aattcagaaa ggatacttta catgaataca ataaagaacg caccgttggc tcttgtcacc
    20881 ggtgcctccc gcggcatcgg taaggccatc gcagaagccc tggccgaagc cggctaccat
    20941 ctgatcctca cctgtagcca taccttgccg gaccttcagg aagtggctgc ctgtttagaa
    21001 gaaaaatatg gcatcacctg caccgccatg gctgcggacg catcggaacc cgctgccatc
    21061 aaaaaagttt ttgcctccct ggaccatctg gatgtactgg tcaacaacgc cggaatctct
    21121 tacattggtc tgctcacgga tatgagtgtc gaagaatggc agcatgtcct gaatacgaac
    21181 ctgagctcct gcttctacac cagccgtctg gcagtgcctc tgatgctgct gaagcacagc
    21241 ggacgcatta tcaatatttc ctccgtctgg ggaaatgtgg gagcttccat ggaagtggct
    21301 tactccgcct ccaagggtgg tgtgaacagc ttcaccaaag cactggccaa ggagctggct
    21361 cccagtaata tccaggtcaa cgccatctcc tgcggtgtta ttgatactgc catgaaccac
    21421 tgcttctccc ccgaggaaat gaacgccctc cgggaagaga ttcccgccga ccgtctgggc
    21481 cagccggagg aagtcgcaaa acttgtcctc cagctcatcc aggcgccaac ctatctcacc
    21541 ggacagatcc ttactcttga cggaggctgg caataacact ctccaccgat tctcccggta
    21601 ccagtgatgc cacgatccgc tccatctctt ctttggatcc agcatcccgg caccggagga
    21661 tcagatgttc cttctccgtc tctgtcagtg ccgcatagcc ttgcatggcc tgttcattca
    21721 tcgccagcgc catgccgaat cctaccgaca gttcattcaa aggaaacgct tctgttccgc
    21781 cgcttccccc agtcaatcca tccattgcaa actcctttcc cgcgttctgc gaagatgcca
    21841 cattttacag gtatcctgtt tttgcataac aatcgtgcac ttgatcgata acataatttt
    21901 gcatatgtta tcgatcaagt gctactgttt agtctgtgca ggaggaatct gcgatataca
    21961 aaacgaaaac tcgctctttg taaatgaaaa tctcacgaat aacggtattc cccgccatcc
    22021 gtgagatttt cagtttggtt tctgtctgtc aggtatccaa cattattttc cggcaagata
    22081 ttctctcata tgttctctga tatgctcgaa ctgttttctc gtctgaggat tccgtgattc
    22141 aaaattcatc agcttatcca gatacccctg tctcttcaca atacggcagc tctcgggata
    22201 gaccagctcg tacaccagcg aaatatgtcc caccacatta tccaccgctg ttttcttcag
    22261 ggaacgcagt acagcgtgct cctcatcaaa gctctgcaac acttccggcg tcacttcctc
    22321 ctggtataat acctcggtgt ccacattgta gatctcttcc agagggaatt ccacattgac
    22381 cttcaagata tcaatcttat ccgcatcccg taagatatgg cagaacattg ccgtacgttc
    22441 gtccagtccc ttcggaatcc ggtaggcgct gtgataaaac accgccgtcc acagtagctc
    22501 atcgcaggaa gcatcttcca cgaaatcccg gatactgatt ccggcatctg cctgaatttc
    22561 ctcggacagg ctttcttcgc ttccggaagc aagtccgtgt ttctcttccc agaccttacc
    22621 aaataaaatc cttgctccgt atttcgcatg atcgatggaa tctgcatcgg taaaggtacc
    22681 atatacccgc tgttgttcaa agcgtcccac atcatggagc agtccggtca gccacgcaat
    22741 gtccacctct tcctccggaa ggtccagaga tctggcgatc tgctgacaca atccgcagac
    22801 ccggtaggtg tgttctattt tcaggcggat catgtcccgg ctgctgtcat aacggtccgt
    22861 gtattcctga aatgctgcca gtgctttttc tctgttgatc ttcattgttc gttgcctctt
    22921 ttgcctctta tctcttatat catgtttcat gactcatgac ctgttcggtt ttttcaaatc
    22981 cattccttct tatctgatgg tttttgtctc tgtcaggcct ttttctctgt cgacgataca
    23041 taggccagct tgtataccgc tacacagatc actgcaacca ccatcagcaa tcccagctcc
    23101 aatgcccatg taggaatcgt ggaggcacac accacagtca gaaagtccac tgcaaaatgc
    23161 agtccgaagg ctgctgccaa atgcagcttt ctgccagtct tcaccgcact gtatacaaga
    23221 atcgacaacg cgatctgcag agcgatcgca ctgatccgct ccaccggggc gagatagaac
    23281 tggtaagccg gcgtggtcca gagaacagag agctgatggt aggttacctc ctgttgtgcg
    23341 ggctccaatg ccgtcatagt cttctccatc aggccaccgt taatcatggc tgcggtaagg
    23401 agattgctca tagaagacat accaaggatc agaaaagcct caatgccgcc atgtccggcg
    23461 ccgtacatca atgcattttc ccgggtaaga tgttttttca ggcagaattt catggcaatc
    23521 caccggccac actcctcaaa aagtgctgct gcaagcccac cgtaaatcgc atacagccag
    23581 atattgtccc ggatcacgtc tccggtcacc gtcagcacaa tggcatgaca gatctgctct
    23641 aagatcatgg caaagcccac gaagataccg cagccgatga aaaatgccgg cacccacgcc
    23701 tttgtcttct tatagataaa gatacctaaa aatatgggta aacccacgga taatagcaga
    23761 gatatcacca tccccaccat gcttgcaccg cttacggttc cccagatcat tgctgtttcc
    23821 gttacactct caactgccat atattctcct atctgagcgc cacgggacgc tctgcgcacc
    23881 tgttatttca ccgttccctc ttgtaagatt ctccggaaaa aggtaagaaa accttcctct
    23941 accgccacct gatccaccgc cgtctcaggc agatcataga aatatttccc gcttccggga
    24001 tccagggaaa tgctgtccag cggaaacccc tctctgcgga tgctgctgtg gggtacatcc
    24061 gtcagccaga atttctcact ttccatgtca ggctccatcg cctcatatac atgttcctca
    24121 tcctgcacat agcagatggc attgcggtat tttgccgtaa gccttccata gcgttttacc
    24181 agtccgatat aatgctccag catctgctca tccgtcagat atacaccgtt caccgtacgc
    24241 acatgtaccc cgggctggat cgcctccggg acatcttcaa aatacaaccc ggaatcacag
    24301 gaaaatacag gcatacgaaa agcttcataa taagcatgcg ctttctgcct ggcattctcc
    24361 agaggtgtat tgcctgtctc cggggcatcg gggatctcca ccccttgtgc ccgcagatcc
    24421 tgcagactga tcagttcaat gccaagtccg gctatgcggt tctgcatggc agctgccttt
    24481 gccggattgc cggtaccgta taacaatttc ataagcgttg tcctgcttcc tttccggtca
    24541 gatcagcgcc ggtgtctgtg ccaggacaga gataatcgcg atcgccgcca aaatcacaac
    24601 aactaccact actgcaacaa tgatcttctt tttcatatat gatacctgct ctttctaac
//
LOCUS       DAWEJW010000032        24615 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_835_length_24615_cov_66.193811, whole genome shotgun sequence.
ACCESSION   DAWEJW010000032 DAWEJW010000000
VERSION     DAWEJW010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 24615)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24615)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24615
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_835_length_24615_cov_66.193811"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         6651 a         5005 c         6359 g         6600 t
ORIGIN      
        1 ttgtactatt attatatgca actttagaaa atatgtcaat ccccatgaaa aatcaaaaaa
       61 tatcttttct gcttatgaga aaggaggtcc cttctatggc aaccacaaga atcatgccgc
      121 ttcatgtcgg caagggccgc acagaaagtc gggcgatcag tgacatcatc gactatgtgg
      181 ccaacccaaa gaaaacagat aacggcaggc tcattaccgg ctatgcgtgt gacagccgga
      241 ctgcggatgc agagttcctt ctggcaaagc ggcagtacat tgccgccacg ggacgagtgc
      301 gcggcgcgga tgatgtgatt gcctatcatg tgcgccagtc tttccgcccc ggagagatca
      361 ctccggaaga agcaaacagg ctgggtgtgg aatttgccaa gcgttttacc aaaggcaatc
      421 atgcctttgt ggtctgcact cacatagaca agtcgcacat tcataatcac attatctggt
      481 catcggtcag cttagaatat gaccggaagt tccgaaactt ttggggcagc accaaggcgg
      541 ttcgtcggct aagtgacacc atctgtattg agaatggact gtccattgta gagaatccga
      601 aacctcacgg aaagagctat aacaaatggc tgggcgatca ggcaaagccc tctcaccgag
      661 agctgctgcg ggtggcgatt gacaacgcat tatcacaaag ccctgttgat tttgaggaac
      721 tgctgaagct gctgcaagag tctggctgtg aggtatcaaa acgagggaaa tcatatcgcc
      781 tgaagctctc cggttgggag aaggcagccc gtatggacag cctgggagaa ggatatggat
      841 tagaagattt gcaggcggtt ctctccggga agaaagcgca taccccacga aagaaagtgg
      901 tcgcacaggc agagccgccg aaggtcaatc tgctggtgga cattcaggca aaattgcagg
      961 ctggaaaagg tgctggctat gctcgatggg ccaaggtgtt caacctcaag cagatggcgc
     1021 agaccataaa ctacctatca gagcacaacc tgctggagta tgcggttttg gaagaaaagg
     1081 ctgcggctgc cacggcacat cacaatgatc tttcggcgca gatcaaagcg gctgaaaagc
     1141 gcatggcaga gatcgctgtt ctgcgtactc acatcgtaaa ttatgccaag acccgtgagg
     1201 tctatgtggc ataccgcaag gcgggttact ctaagaaatt ccgggaggaa catgaggaag
     1261 aaattctgct ccaccaggct gctaagaatg cctttgatga gatgggcgtc aagaagctgc
     1321 ccaaggtcaa agagttgcag acagagtatg cgaaattgct ggaagaaaag aaaaagacct
     1381 atgccgagta ccggcgttcc cgtgaggaaa tgcgggagct tttaacggca aaggccaatg
     1441 tggatcgagt gctgaaaatg gaggtagaac aggatgttga aaaagaaaaa gaccacggcc
     1501 agcggtaagc tggtcctggt caaaagcgtt cgcagaacgc gcagccacag aatgaaattc
     1561 tgtgttcaaa ggggcttggg ggcgctgccc tcaacaagca agcggagagg aaaatcacgg
     1621 agggattttg cgctctgcgg gtcggtgtgt attaacaccg gcattgcttg cctcttttac
     1681 ccggaaatga aaggagccag tgaggaaaca gtcaaatttc actcaccggc tcaattcgtt
     1741 tcaaatttct cggatagttc tgttagttct ttcgcggtct cctgcgtatg gtgcaagagg
     1801 gttttacgca attctgacgg acagctgcaa taaagcatct tcatctgatt ggctccctca
     1861 tcttctggag aaacatctgg attgatcagg gtgtccagag agagcggaag cacctttgcc
     1921 aatgccttca aaatcaaata ggatgggttt atttcccctt tttcgatttt ggcgatttgc
     1981 tttacagata catgacttag atccgcaagt tcttgttgtg tcaggtcttt tttctttcgg
     2041 gcttcccgca ttttttgccc caaagcggtc aaatcatcaa tcggcataat cattcacctc
     2101 aaatatattg tatcgtgccg atttacacaa aggaataacc ttattatgcc tattgacaga
     2161 tatgattata ctgattttat gagagggaat agggaaagtg cagttgtatc cttgatgagc
     2221 tatgttacaa tgctaatagt aaggaaaaat aaaaaatgtt tttgattttt agatttagga
     2281 catttctgta acattttgtg tttatcctat cagtatagtt gaaaaataag gaaggaagtg
     2341 aatgtcttga agaagattct aattgtagag gacgatcagc ttttgaatcg gacattggca
     2401 tataatctga cctctgatgg atatgaggtc atttctgttt ttaattttga cagcgcggta
     2461 agaaagctgc gggaaaatga atttgatgtg gcactgttgg atattaacct ccccgatggc
     2521 agcggactgg atttgtgtga agaaatccgg aatcgcggtc agcataccta tatcatcttt
     2581 atcacagcca atgataagga gagcgatatg ctcaagggat atgaagtcgg cggcgcggat
     2641 tatgtgacta agcccttttc tgtgacagtg ctttgtaaaa aagtggcggc ggtttttgca
     2701 aatctggaac tgcggacacc ccggcatgat ctgtttgatg atggttttct gaaaatcgac
     2761 ttctccgaac aaagtgcgtc ccttgccgga gaatccctgg actttacacc gaaggaatac
     2821 cgtaccctgt tcctgttcgt aaagaacccc cggattattc tgaccaagcg gcagatttta
     2881 gagaagctgt gggacattga tggagacttt gtggatgagc atactctaac gacgatcatc
     2941 agccgtatcc gcaaaaaaat cgaaacagac gagaggaaat atattaagac agcttatgga
     3001 atgggctatc agtggatcgg cggtgagcct cgatgaagct gcaaaattta tctgtcaggg
     3061 catttttgag aaaaattgtg gcagggactc tgctcactgc cgctttgctg ctgttgattt
     3121 ttttcctgtt gacgaaagat gtgcgtgttg tcatctgcgg cattattttg acagctgcat
     3181 tttccatttg gggcatggtt tttctgcact attttcaaaa gaaactttct ctgttcaccg
     3241 atggtttgtg ccagacattg gatcacatga tggacagtac agaccggccc caggtagatt
     3301 atgaagcgga aacactgctg tcccggatca gccaccgtct ggaacggctc tataatgtga
     3361 tgcagaaaac cagacatacg gcagagggag aaaaggaaga actgcaatcc ctggtatcag
     3421 atatttccca ccagaccaaa acccccatcg caaatttgaa gctgatcaac gatacgatgc
     3481 tgacaaggcc attgacagaa gaaaagcgca aggagttttt gcaggccaca ggcacgcagc
     3541 tggataaatt ggattttctc attcagggaa tggtgaaaac ctcccgtttg gaaacgggtg
     3601 tcattacgct ggagaaacag gatgctgtaa tcggggatac gctggtgagc gccatcaatg
     3661 gtgttctggc tccgatggaa caaaaagaga ttagcctgtc ggtggactgc ccatctgatc
     3721 tgacaatatc ccatgacagc cgctggactt ccgaagcatt gtttaacatt ctggataatg
     3781 cggtaaagta cacctctgcc ggtggcagca tccaggtgag ggtacgggat tgggaaatgt
     3841 atttgaggat agatgtgacg gatacaggca gaggcatccc ggagcattct cagggcacga
     3901 tcttcaaacg cttctatcgg gatgaagcgg tgcatgatat agatggggtc ggaatcggct
     3961 tataccttgc ccgtgagatc attaccatgc agggtggcta tattaccgtc gagtctaaag
     4021 ttggggaagg atcaactttt tccgtgttcc tgcccataaa ataattctga ggcagtctgt
     4081 tttggcaggc tgcctttttt gaaaaatttt tagaggacaa tggattttag gacaattctg
     4141 caacatttgg gatttatgct gacagcaaca tcatgaaatt ggaggtggcc ttatggaaat
     4201 cacaaagaca ttactcatca atgcagcttg cttgctgatc ccgccggtcg ttgttgttct
     4261 gctgctccgg cacttctttc caagaaaaat cagatggtca gctggtgtgg tggctctggt
     4321 agatctggtg atcttctgga cagatatttc ttattatgag agcgtttggc tgacgctgtt
     4381 ttttgttgta attcagttag caatggtttt gcttatatct atccggcttt ataaaaaaga
     4441 cacacgaaaa tgaattgtcc taaaattgaa gcaattcacc cttaaactgt tgagcctttg
     4501 ggatatttct gcaacatttg ggggttagga tgacagcgaa aggagtgaat ggctatgaca
     4561 ttaccatttg aaaatgatac ccgtattatt gtaaagaagt tggcaaaacg cagcttacag
     4621 gcagataaac ggcggaatat ttttttaatt ctaaccatca ttttaaccac agcgctctta
     4681 tcaggaatgt tttttaccgt ctttgccaaa caaagagagt taaaagacaa catccgggga
     4741 cagtaccaag cagttgtgat ggagactaca ccggaagaaa tagaccgcct ttcagtccaa
     4801 tctgaaattg aacaatgggg attatctcaa aattttggta ctactcgcta ccaagacagc
     4861 aatcttacag tggaatacgc tgacgaaggt tggatggttt tagggaaaaa gccatcctat
     4921 atagggacat tcccgcaagc agaaaacgag attttagtag aacgggcatt tctggattat
     4981 tttgaattgc cacaagaaac tgggcaaaca atccgactgg atttaggaaa cggggaacag
     5041 gattatattg tttcgggtat cttacaaaga gaaaacactt cccggatatt ttcggttatg
     5101 gtgtcccgca cctttttaga gatgcaggca gaaggtgagc ctctttttga atttcggttt
     5161 cgctttgttg gtgctgaccg ttcagacatg aacgaactaa aaacgaatat tacttccttt
     5221 ttagtagcaa acaatattcc agaaaaccgt gttttttaca gctcaaacta ttttgatatg
     5281 cagggatttc atagcaacag cgtttatgtt tgtattccta ttgcaattat ttttttaacc
     5341 gcctgcggtc tggtgattta tagtatcttc ttcatttcta tcagaggaaa actccgggaa
     5401 tatggaagat taaaagtttt aggtacgaca cctcgacaac tccggcggat tatccgacgg
     5461 gaaagttttt ggctgtctat atgcagtatt ccgattggtc ttataattgg cgcactgttg
     5521 ggatttatag caagaccatc atattgggaa tggaaagaaa atttgttgct tgccgtttgt
     5581 gtagcgggat ttaccgaact gattgtgatg ttttctaccc acgcaccgat tcgtctggcg
     5641 actaaagttt cccctattga ggcagtccgg gacagtgggt atcagacaga agcgcctaaa
     5701 aaatcttcca gaaaatcagg aaaacatatt tctcctgtgt atttggctat gatgaatttt
     5761 cagcgaaatc caaaaaaagc agtgctgact ttgctttccc ttagtctgac aggggtattt
     5821 ttgttcagtg cagcaaccgt actccgttcc gttaatgtca acaatatggc ggccagttcc
     5881 atgtatgacg attgcaacta tacgatcatg tgggaagcct ccccggaaga actcttagaa
     5941 atctcccgcg aaaatcccct gacaccggaa ctacgggaaa aggttcttgc agtagatggc
     6001 gtaaaatcta ttatcccccg cacttcttcc gcagctattt tgtcattgcc gaatggtgtt
     6061 tctgatgact ttgagctaca tactttcaca agggaagaaa tggaggaatt gatcccagag
     6121 aagatgatgg tggatggaac atccgactat gatgaattag ttaagaatca tggcatagtc
     6181 atcacggatt ctatggatga tcctatgctt tccatgcttt cgggttatca gcctaaagtt
     6241 ggcgatatta tcacttttca accttatggt ggagaaccta cggaacttac agttatggga
     6301 attgccgaca gcaaaaaggt tacgaaaacc acaggaatag ccatgtttac tttgccagta
     6361 gacttagcac agcagcttta tcctgatgta gagaatatgg atcgcgtttg gaatgtcttt
     6421 actgaacagg ataccgatag tctacggaaa gaactctttt ctttgttgga cgatccccgg
     6481 ctgaatataa tttcccgctc tgattatgga gaggaatttg aagttgccct ccaaagcgtt
     6541 atgcttctga tttatggact tctttgcttc ttgttcctgt ttgctttggt taatctggta
     6601 aacaccctga taacgaatct tctttcacgc caacaggaat ttggagtatt gcagtctgtg
     6661 ggaatgtcgg gtagacagct ttctaaaatg ctgacgatgg aatgtctgtg ttatatcatt
     6721 gccacactcg tgattatgct gctatttggc ggattgatag gggcatttct ggtggtgctt
     6781 attacaaaag ctaatatcgt cgggccattg gtttaccagt tccccatttg ggaattgcta
     6841 acttttgcag cagcacttct tttgattttg gttttatatt ctgcctttgc agtccgctat
     6901 atgcggaggc aatctttggt ggaacggatt aaggccatga gctgataaaa aagtatctta
     6961 taaaagaatc acagaggaca attctgtgtt gctggccgcc gtaaaagtct attgtggaga
     7021 gattgcggga aatcgaatag atagtttgaa actactcgtt caataagaaa atgaattgtc
     7081 ctaaaataga agcaattcac ccttaaactg ttgagccttt gggatatttc tgcaacattt
     7141 gggggttagg atgatagcga aaggagtgaa ggactatgac attaccattt gaaaatgata
     7201 ccagccggat tgtaaagcgg ctggcgaaac agaatatgaa agcaaaccgg cgtacatcca
     7261 tttctatcat ggtagccata ttgatcgcat caactttttt gtgttcgtta tgcacattcg
     7321 tccaaagcta ttggaatcaa agtgtgcagc aggagattgc agcctacagt gattgggatg
     7381 cacagctttt ggagattcat gccggccagc tggatttgat tcggaataac gaaaatatcc
     7441 agagcatcat ggtaaaagga gataatcaga ccgccttatt gccgactgca tccggcctgc
     7501 cctatctttt gatccaaaat tgtgatgggg catattgggg cggtatgcgg gaaaagaatc
     7561 tgatcctgcg cggacgggtt ccccaggcgc cgggagagat tgttgttgga aaaagttttt
     7621 ttgagcagaa tccatctgtt gagattggag atcggctgaa cctggatttg ggcgaacgaa
     7681 ggattggaaa tacaactgtg gattttcttt cccccattca aagcggggaa gaatttgtga
     7741 aaacagaaaa agtgcagtac accatcgttg gtgagattga tatgactgtt tccagcgcat
     7801 acaatggcta ccctgcgtat gggtggctgg acacggccac attatcggag gacacgggga
     7861 tcgttgcgta tcttcaagtg acgcagccgc gaaaagtcta tgaaatcatc ccgcagatag
     7921 cagaggacat tggcttgcag ccggatgaat ttggtgacta tcccttccgt taccacacag
     7981 ccctgcttgg gatgtatgga atttatgcgc cgggacattt cctaagcagt gaccttccca
     8041 aacttgcgct tgcgctgggt ttggtcatgg cagcatctat ggcggtattt gcctatatca
     8101 ttcggggcgc tttttctatt tctgcgaaac gcaaggtaaa ggagctggga atcttaaaat
     8161 ccattggaat gacgcccagg cagatccgca tgatgatcgt atatgaggcg cgctggctgt
     8221 ctgtgcttcc cattctggtt tctgtgggac ttggatacct tttttcctat ggcgtattgg
     8281 cagcatactc tgacctgacg caggaagtga ccgggagccg gataaccgct tccttttccc
     8341 cgtgggtcgc agtggtttct gtgattcttt cgttcctgac ggttcgcctg gctgcttctg
     8401 gtcctgcccg gcagatggga aaacttcgcc cgattgaagc ggtcaaagaa agctggagca
     8461 atccttcttt gaaaaaatca gcaaagcatc cgatcctgaa aaagtgcttt ggattccttg
     8521 gaaatatttc tgcaaactcc atgacggcta ataagaggct gtttcgtacc tgtacggtaa
     8581 cgctgagtct gtgtatgctt ttgatgttca gttttctggc tgttttttcc gtttcagata
     8641 ttaacaatac aaaagcagaa caggacagcc actttaatgt caatcttacc atggagtctg
     8701 gtcagaaaat agagccagcg cttatggatg aattgaagca gctgcctcat atagaagaac
     8761 aggccaccta tacgatggcg aactgtgcga tctgggtttc agaaagtgag ctgtcggaag
     8821 aatttctttc ctctggagga tttggcacaa aagcggctgg tgagtatgta gcaaagcgcg
     8881 atggtcgcta ccgtatccca tgtgtattga tcggtctgga acaggacgcc tatgaaaatt
     8941 atctcatgca gtcaggggtg ccttattctg aacagggtgc tgcgctcatt gtcaattctg
     9001 ttgtgaaaaa tcctgactcc agaggttatg aagccaaaaa agatatggtt ccatatctaa
     9061 aactcaaaga aggtcagtct ttagaagtaa cagagaagtt cctggactcc attcaggggg
     9121 attaccgttt tgatgtgacg gtgtcatctg cactttctga aatgccggag attgggcgta
     9181 atatggcctt ttatacattg cccattctgg tgccgatgga gcgatactat gagattatcc
     9241 agaatttcgg agaggatcgt gcagtttaca attacaggac ttacatgaat cttctcgtgg
     9301 aagatgggct ggatgccgaa gtacaggcgc aggcagagca tatctgtggt acttatctgg
     9361 ggacgagtga tttctatacc tccagcaaga cacagcgcgc tttggacaga gagcggctga
     9421 ccgatgcgac catgctgatc gtttacagtt tgactgcact atttggtatc attggaatct
     9481 catcggcagc agtggcgatt ctgaacagtc tgtatcagag aagaaaggaa tttgccatgc
     9541 tgcgttctgt ggggttggat agaaaaggtc ttgaccgttt actgcatata gaagggttct
     9601 tccttggagg aaagcctttg gtcattggat tgccgatcct gtttttgatc gccgcagttt
     9661 tgatgtggat gcaggatgtg actttcatgg agttcattca ggtatttccg ttattagggc
     9721 tggcagccta tattgtcctg gtgttagtgg tcatcagtgg gatttacagg gcggcttcca
     9781 gaagaatccg cagggatatt attgtggaag tcttaaagga tgaaaatgtg tgagtcctac
     9841 tgcaaaatgg tttaccatta tgccataacc caaagcagga ggcaaagacc atgaaaaaaa
     9901 cgatggtaat ttcatttata gctgctgcga tgctattgat actatgtacg gcttgtcatg
     9961 tagcagagag ccacaatgag gtactggcac aagaaaacac tgttaacgga ggatatacca
    10021 ttacagatat taccgacccc gttgaagttc aaagctacat tgagcagcaa ggcggtgaat
    10081 acactgccga tattatggct gttcatcagg ttacatttac cgattatacc gtaaacgaca
    10141 ttacagatcc ggcagaaatc aaagcctatc tggaaagtca ggggcaagaa tataacgaga
    10201 atattaccgc tattcatcag attactttca ttgacgggga agcgacacca gagggttccc
    10261 tttgcgaagt tgaaaaatga attgtcctaa aattgaagca attcaccata gattttcatg
    10321 ccatttagga caattctgca acatttgccc tttaccctat actcaacaaa tataagaaag
    10381 gcaggtaact gttatgaaaa ttttgcagac aactgatctt aaaaaatact acggcacaga
    10441 gccgaacatt acccgcgccc tggatggcgt gaacttctct gtggaggagg gcgaatttgt
    10501 ggcggtggtc ggcacttccg gctccggtaa gtccaccctg cttcacatga tgggcggtct
    10561 ggacacaccc acctccggca gcgtgatggt gcgggataag gagctggcga agatgaacga
    10621 tgagcagctt actatcttcc gccgccgcaa tatcggcttt attttccaga actacaatct
    10681 ggtgccgatc ctaaatgtcc atgagaacat cgtcctgccg gtggagctgg atggggatac
    10741 ggtggatcag aaattcatgg cggatgtggt gtccatgctg ggactggagg ataagctgaa
    10801 gaatatgcca aacaacctct ccggtggcca gcagcagcgt gtggctatcg cccgcgctct
    10861 ggtgtcaaag cccgctatcg tccttgccga tgaacccacc ggaaacctgg acagcaggac
    10921 cagcgccgat gtgctggggc ttttgcagag aacgagtcgt gaatttaacc agacgctggt
    10981 aatgatcaca cataatgatg ccattgccca gcttgcagac cgtatcgtgc ggattgagga
    11041 tggcaaaatc gttggataaa ggaggtggca gacatgacat tgccttttga aaatgataca
    11101 gggccggtta ttaaaaaatt ggcgtcacga agtattcaga cagataagcg gcgcaacctg
    11161 tttgtgatcg taacgattgc tctggcagcc gctttgatgg cagcgatttt ttgtgcagga
    11221 gcgggaagtg acagaaaact ggaggcggac attcggggac agtatcaggc ggttgtggtg
    11281 aattgtgacc gtgatacgat tgaaagactg aaagactgcc cggaagtgga acgctggggc
    11341 ctttcccaga attacggttc tgcccgctat ggtgacagcg ttctgactgt ggagtatgcg
    11401 gatgtcaact ggatggaatt gggtaagaag ccttccttta ctgggacact tccgcaggct
    11461 gccaatgaaa ttctggtaga acgggcattt cttgattatt ttgaaattcc cgcagaagtc
    11521 gggcagacca tagaagtgaa tcttggaaac ggaaagcaga cctataccgt cagcggtatc
    11581 atggatgtgg agaatgactc ccgtatgttc cagctttatg tatctgaagc atttgtggag
    11641 gagatggcgc agggagagcc gctgtttgaa tttcggcttc gttatactgg tgctgatagt
    11701 atggagctgg agcagctcaa ggcggatatt gcagcattcc tttctgccaa cgatgtctca
    11761 gaggatcaga ttttttacag ttccaactat ttcgatatgc agggctttaa gtccggcgtg
    11821 atgaaatatt acattcccgt ggcaatcctt ttgctgattg cctgtgccgt ggtcatttac
    11881 agtatctttt tcatttccgt aaagggcaaa atgcgggaat atggacgcct gaaagtgatt
    11941 ggcacaacac caaaacagat ccggcggatc gtgcggcggg agggattgct gttatctctt
    12001 tgcggtatcc cgctggggct tttggttgga ggggccatcg ggtttgccat tttcccggca
    12061 cattggagct ggatgggcaa tctgccctat ctggctgcga ccgcagcagc ctgcgccctg
    12121 acggtatttc tgtccattca tgcgcctgtc cgcatggcgg cgagggtatc cccaatcgaa
    12181 gcggtccgct ccagtggtta tgaaacggca acagaccgga aagatcagaa aaaccaccgc
    12241 attacgccgt tctctatggc acagatgaac tttaggcgga gcaggaaaaa gacggtcatc
    12301 actctggtat ctttgggatt gacgggtgtg tttctggtaa cagccgctac ggtacttaac
    12361 tccatcagtc ccgaaaagat ggcgatagaa gctatgggag atcactgcaa ctatgaggtc
    12421 agctggatgg attcgggagg cgcggaagga ttgcctgcca tcgcacggga aaatccattg
    12481 acagaagaac ttcgtcagga gcttcttgcc atagatggtg tggaatctat caccagccat
    12541 gaagtaacat ccgtaacggt gaaactcccc caggaaagtg ttgcattaaa gttccctgtg
    12601 tttgacagag agcagatgga acagtggctg ccggaggaaa atctggaaca gggcagcgca
    12661 gattatgagg agcttgccgc gaacaacggt gtggtcgtga ctgattcgga agatcatctt
    12721 ttaagtatgt tttatggcta tacgcctgct gtcggggatg tgctgacctt tgaatccatg
    12781 gacggtaaga ccatagaagt aacggtcatg gggattgcga aaccgtcagt gacatcgggc
    12841 accggcgcat ggggcctgtt tacccttaca gaagaactgg ccgatcagct ctacccggac
    12901 attgagaaca gagaggctgt ttggaatgtc catacatcgg aggactcgga tgcgctcagg
    12961 gcgtctattt tcagcctgct ggaaaatccg gtgctgacgg tcttttccag agcagaccat
    13021 gcggcatccc tggaatcgca gctaaagatg atgacccgcg gtgtttatct gctgctggca
    13081 ttcctgtttg tgttctccat ggtaaatctg gtcaacaccc tgatgaccaa tctgctggca
    13141 aggcagcagg agcttgggat tttgcagtct gtgggaatga ccggaaagca ggtttcccgt
    13201 atgctgattg cagaatgcct gtggtacgca ggggttacgg tatttttgtc agttggcatt
    13261 ggcggaatcc tgggctggat ttttgactat gtgatcagca gttttaatat ttttggggaa
    13321 ctgtcctatc aattcccttt gatggaaacg gttgtttttg ttctggcact tttggtcgtg
    13381 accggaatct tctctgtggt tgctgtccgt tactctaaac ggctttccct tgtagagcgg
    13441 atcaagacca tggactaacc agaggcggct tccgatgtga agccgccgct ttgctgacag
    13501 cagaaatata agacttaaag gagatgatca aaatgacagt accatttgaa aatgatacag
    13561 gaaatattgt caggaaactg gcaagaaaaa gtttgaaaag tgagaagcgt cggaatctca
    13621 tggtggtgat cgccgttgcg ctggcatcct gtctgatttg tttttcgatt gtgatggctt
    13681 tgtctacccg gcagatagag aaaaatcatg tggaggatac ctatgaggcg gtctatacca
    13741 ggattaccga agaagatgtg tccaccctga aggggctgga tgacttctca agggtaggag
    13801 agtattatat gctggctgtg gaacctgcgg agcagggata caatgcctcc tatatttatt
    13861 gtgatgagga aacgatgtat attgcccgtg accagatgaa gttgctggaa ggccgactgc
    13921 cccaaaagga ggatgaggtg gtggtgagcc gttatttcct ttccaactat ggtgcagacg
    13981 ccgggatagg tgaaaaggtt atgctaagca gcgagagctt tcacggagaa tacacggtga
    14041 ctggcattat ggaaggctat aaggaaaaag aggtaaatgg atcttccatc cttctttcca
    14101 aagaagccct gaagggatgg agcggctatg accccgctga ttatcgagcc tatgtgcatt
    14161 ttaagaatga gcagcaaatg gatgaaacag agctgactgc ccgcagcagg gagattgcca
    14221 aggaatatca gttggaaatg ccgatcatga acctcagcta tatgaagttc tataagcagc
    14281 ctgtgaatgt cagtatgctg gcgctggttg ccggaattgc agttctggtt atcatcggag
    14341 ggtatgttgt gatacaaagt atcttccgaa tttctatcaa tgacaaaatc caaagctatg
    14401 gacaactgcg gaccatcggc acaacgccaa aacagatccg ccgtattgtc aaaaaagaag
    14461 gccgtttctt aggatgggct ggcattggta tcggtatttt gctgggatgt gcagccgggt
    14521 ttgcgctgtt tccccgtggg tttaatctgc tattttatgt agccgcgatt gttttgactg
    14581 cactgcttgg ctggtttatg gtatccattg cgatccgcag accggtaaag attgccgccg
    14641 gtatctcccc gattgaagca gtccgcttta ccggcaatca gagcggaaag gcccacacac
    14701 ataagaagaa tatgcggtta aatcccctct ccatgggaat tgcaaatttc aggcgtgacc
    14761 gcaaaaagac gatcagtatc gtggcttccc tgagtctggg cggggtaatc cttttggtga
    14821 cagcttctat tcttttggtt cgttcgccgg agcggatcgc aagacagtat ttcccggatg
    14881 gggattataa aatctatatc cattctgaaa aaacagaata tgaggtgatg tcaaaaggaa
    14941 atcctttgaa tgaagcactt cggcaggagg tgctggctgt ggacggcgta acagatgtga
    15001 tagagaatcg tcaggcggtt catgtgcggt ttgaaaatga ggagagttct tccggcggaa
    15061 tgtgtgatct gttgagtgac cgaaacaggg ataagatgga agcggcgctt gtgtccggaa
    15121 ctttacctca agattcccat agtattgcac ttgatatgga atatgcagaa gatatgatcg
    15181 gtgtagatgt ggggtcagtt ataaaactta ccatagggga tcaagagatt cctgtcacgg
    15241 tttcgggtct gttcataaac gatgggctta aaaatggtca tggtcctctg gcattggaca
    15301 gcacctgcat ggttgcaacc aaagaattgt ttcaggaagc aatgcctatg attgattgtt
    15361 ttgattattc ctggagcatt gtcagcgatc ccaaaaaggc ggagcagatt gaaaacagtc
    15421 tgagtgcgat catttcttcc aatccagatc ttgacttgga taccattggg atacataagg
    15481 attatgcgga aatggaaaat cgggttgttt ttggagggtt tcaggcgctg tcctggctgg
    15541 tattcctctt tggcgtggtc aatctcatca acaccaccct ctccaatcag atgtccagaa
    15601 agagggaaaa cagcgtcttt cgtgccatcg gtctgaccag aaaacagctc tgtcaaatga
    15661 ccatctatga gggaatttgc tatgcgtttt ctgctgcgtt ggcaaccctg gcagtcggcc
    15721 tcccgattgc agtgatattt gcccgaaaat tcagcgaaat gacttttcat gtagcaatgc
    15781 catattcctt ccccttcctg caaatggggc tgttcgttct ggtgctgttt ggcctggagg
    15841 tgattctgtc cttctggaca atgcgcagac agaaaaatca atcgctggtg gaagaaatgc
    15901 ggtcaatgga atgacctttt tgaagtggcg gggcggcagt gcgctgcccc gctttttcta
    15961 ttattttaat aaattaaaat caggggtgga tatatgaaaa agatactgtt gattgacgat
    16021 agtgatacct atatatggaa tctgaggaag tatttacagc gccggggcta tccagtgaaa
    16081 acggcttcca ctttgaatga agcgcggacc gtcatccaag aggaaatgcc gctggtagtc
    16141 tgctgtgatc tcgacctgcc ggacggttcc ggcgtggatt ttctggacga ggtgcggacc
    16201 gcagacaagg agctgccttt tattctggcg tcctgccatg acaaggacga ctacgaacaa
    16261 gaggctatgc gccggggcgc gacgctgtgc atggacaaaa tgaaagggca gttggtgcaa
    16321 gataagctgg tggaatacgc ctaccggcag ttatccggcg aaaaggcccc gacttttcac
    16381 aagctgctct ttgtccatac ggaagatacc aacgccgaag tgctgcgggc tgctatgctg
    16441 caaaagggct ttgacctgat tctggtttcc tcgattgagg aggccaagcg ccatattttt
    16501 gaggataagg aaattgaact gatcttgtgc aatctggaac tgccggatgg cacagcaatg
    16561 gagctgtttc atactctgcg gcgggtggct gggatgttcc aagtgaagaa tccccctgtc
    16621 cagcttctgc cgttctttat cctcaccgag aacaatgacc ttgccacgga atatgaatac
    16681 cggcatgagg gcgtgaacga ctatatcacc gccccggtca atatcccgga gctgatccgg
    16741 cgagttctgt tctttgtgga atgaagcttt tttattcggc ggttcataag caaggccgcc
    16801 atttttaatt catttatctt caatgtttgt tctgaatatc gtataatgta ggggaacggc
    16861 tgtggtttca tagagtggtg aggtgtttat atcatgaatg aacgagaaaa aacaatccga
    16921 ttatggtttg atatgtggct caatcagcag gatatgggga ttgatgatat ttttacagaa
    16981 gatgtgatct atacagaaag ctggagtccc cagtataaca accgaaaaac agtaaagcat
    17041 tggtttcagg aatggaatac ccgtggcaag gtggttattt gggaaatcaa gcaatttttt
    17101 cataagggag atcaaacgat tgtggagtgg tatttcaaaa atgaaatgaa caatggaagt
    17161 atagaggaat ttgatggaat ctcgctggtt gaatggacag aggataatag aataaaggcg
    17221 ttaaaagaat ttgggtgtaa tcgcaatacc tataatccat accaggaagg cggtactcct
    17281 caattcaaag cagaaaaagc gaattggttt tgaattgagt gcttatggag gtaaaaatat
    17341 gatgtatcgg acagaattag tagaaggaat taccgtggaa aatgtaacag agaaaatcaa
    17401 tgcaaaaata taagaaatgg aaaaggaaag ctatcgactt gttacgatgt ctttttgggg
    17461 aaccgaaaga actgtactgg ttttcaaaaa agggttaaag ggtagtttgc tttaacacat
    17521 agtttgcttt tgaactgtcg tctatggtgc gttgacagac tggtaatctc gactattcgt
    17581 taaaggactt tattgtggac tgttcgagca agccattggg ctttgaggtg acaaatatga
    17641 aagtattggt tagtgcttgt attatgggag agaactgtaa atataatggg aaaaacaaca
    17701 aaaattcagc agctatccat tttttaaaag ataaagaagt gatttctatc tgcccggaag
    17761 tattagcagg aatggaaact cccagaccct gcgcagaaat tgtgaatggg agagtagttg
    17821 atgaaaacgg aaatgatgtt agtttagaat ataataaggc agtggcagtt gcattatcga
    17881 aaattcagaa tgaagagatt gatttagtta tattacaatc cagaagccct acctgtggtg
    17941 ttaatcagat atatgatggt agcttcactg gaaagttagt ttctggaatg ggtttgtttg
    18001 caaaggcatt aaaacaaaaa ggatatcatg tcattgatgt tgaagaaata taagtttacc
    18061 atttttcatt tgttgaggga taaggttcct aacgcattaa ggctgcattt ttatattcgc
    18121 ctgatgcaaa tgtcaatggg agcatgagcg actatatcat agaatattat ttttttcacg
    18181 gaggctgata tgaaagaaaa aatattgata atagaagatg atccgttgat acggaatgaa
    18241 ttgaaaacgc tgctgcaaag caatggctat gaagcggcgg ctccggaaga tttttctgat
    18301 gtgattgatc ggatcaaagc cgaacagcca cacttgattt tgctggacat caagctaccg
    18361 ggaaccagtg gcttttctct ctgcaccgag gttcgcacat tttccgaggt gccgatcatt
    18421 tttgtgacaa gctgcaacac ggatatggat gaactgaata gcatcatgct gggcggagat
    18481 gcttttatta cgaaacccta taacacagcg atcctgcttg cgaagatagc atctctgctg
    18541 aaaaaagcct atcccacgca gcaaagggaa cagatcgtct atggagatgc agtgctgcat
    18601 ctggaatcca gcagcctgga ctataacgga catagcgtag agcttaccaa gaacgaatta
    18661 aaaattctct attacctgtt caagaacgga gggaaaatct gttctcgtgg agatatgatt
    18721 gagtatttgt gggacaatca gctatatgtg gatgacaatg ctttgagcgt caatatcaac
    18781 cgcatccggg aaaaactggc gggcatcggt ctgacggatt ttatcaagac aaagcaccga
    18841 caggggtaca cgatatgaac agcagacgat attggaaaaa caggctgcca tttctgctga
    18901 cgaaccttgt ttgtatggct gcactcactg tatttttgct ggtgtgcggc aattcggttt
    18961 ccgcagtagt attgatcctg attgtatggg cattgatttt gctgatggga cttgtcctca
    19021 cttactggaa acgaaagcgg cagatgaaaa aacttctgga tatggcagag cagctctctg
    19081 agagatacct tatttccgaa gtgatggagc tgccggagca ggctgaggat caggtttact
    19141 atcagctttt gaaaatggct ggaaaatcca tgttggagca gattggggag gtcgagcggg
    19201 aacgcctgga gtacaaggaa tacattgaac aatggattca cgaaatcaaa acgcccatta
    19261 ctgccatgaa acttctatgt gaaaaccatc ggatggactg gacaaaagaa cttctgctgg
    19321 aactggaaaa gaccaatcgc tttaccgaac aggctcttta ttatgcccgc agtgagcata
    19381 cagagaaaga ttattctgtc cgggaaatgg cactgtccca agtggtgcat caggcgattg
    19441 cagataataa atatctgctg ctccaaagtg gtatgcgcct ggaagtggag gaaatgcagg
    19501 atacggttta ttcagatgaa aagtgggtgc gttttatcct aaatcaactg attgccaatg
    19561 cggtcaagta tcgtactgaa cagccggttc tccacatttc tactcataaa cggcaggatc
    19621 aggttgttct tgtcgtggag gacaatggaa tcgggattgc tgcgtccgat ctgccccgta
    19681 tctttgaaaa gggatttacc ggtcagaatg gtcgtctgat tcagcagtcc acgggcattg
    19741 gtctgtatct gtgcaaacgg ctctgtgaaa agctgggcat tggcattacg gcggagtcgt
    19801 cggaacatgg cacagccatt tccctttctt ttcacattaa ctgtctgatt catgaagtgc
    19861 agaactgaaa agcgttctgc acttcttaca tttttgttag aactttgtaa gaaaacttga
    19921 tacaaacgaa gccgctctta ttctacaata gagatcagag gtgataacaa tgaaagaaat
    19981 tttgaaactg gatcatatcc aaaaatatta tggaaattgc gggaatgtta caaaagcaat
    20041 tcaggacatc agcttttccg ttcaagaggg agaatttgta ggaatcatgg gagcatctgg
    20101 ctccggcaag actaccctgc tcaactgtat ttccaccatt gataccgtca gtgcgggaca
    20161 tatctatctg gatgggaccg atgtaacgga aatcaatgaa aaacagattg cccggtttcg
    20221 ccgggaaaat cttggatttg tgttccagga ttttaacctg ctggacacgc tgaccatttc
    20281 ggaaaacata gccctggcgc tgaccatcaa caaggttccc gcaggcgaga ttgatgggcg
    20341 ggtgcgggaa atggccggaa agctgaatat cacggatatt ctggacaaat acccctatca
    20401 ggtgtccggc ggccagaagc agcggtgcgc ctgtgccaga gccattatca accagcctaa
    20461 gctgattttg gcggatgaac ccaccggtgc gttagacagt cactcgtccc agatgctgct
    20521 ctctacgatt cagagtatca acgaagatct cggagccacg atcctgatgg tgacacacga
    20581 cgctttctcg gcaagctatg ccaatcgtat tctctttttg agggatgggg caatctttac
    20641 agaaattttc aaaggcagcg attcacgcag gactttcttt gaaaaaattc tggatgtcct
    20701 caccatgatg ggagggggtg tgagtgatgt acgctaaatt ggcactccgt aatatccgca
    20761 gaagcctgcg ggattatatc atttactttg tgacgcttac cttgactgcg gcattgatgt
    20821 actcattttt ggcattgggc ttctcatcag atgttcttgc tatggcagaa aatatgtcga
    20881 tgctgactac tggaattctg cttatgtcag ctctggttgc ttttatgtcc tcgtttgtaa
    20941 tcggttatgc gatccgtttt atgctgggac gacggaaaaa ggaatttgca acctacgaac
    21001 tgattggcat ggaagcaaag acagtacgaa atttgttcct tgctgaaaac agcatcatcg
    21061 gcacaggagc ctttctgctt ggttctttgg ttggtacagg actttccggt ttgctgaatc
    21121 aggtggtaaa aaatatcttt gaggtgccgc atacctatca ggtttccttt tctctccagg
    21181 catgggcagt aacatttttg ttttttgcct tgatgtatgg ttttgggatg ctccgagcag
    21241 caaagattat ccggcatcag aaagtaatcg atttgctgta tgataaccgc aagaatgaag
    21301 aataccgttt caaatcattc cgccatagtc ttttggtagt attgctttca atagccgcaa
    21361 tggttgcagg cgtgattttg cttgggcgga tgcttcagac acaaaccaat gaagcatttt
    21421 tgtaccttgg cggagcctgc ctgctcattc tggtgggtgt ttacgagctg catcgccaga
    21481 ttccgctttt gctgcaccga tttgcaaaac aaaatctgcg ccataagtac aaagaagaaa
    21541 atctgttctt cctggggcag attgggcggc gcatccattc tgctggccgg acaatggctg
    21601 tggtggcgat tcttttaacc atatccctgg caaccatgtt tgtggggctt acaatgggag
    21661 caggctacaa agccaacatg aaagcctatt atccctatga tgcaggcgtg gcgattgatg
    21721 cacctttgga aaaatccaac atggattcca tcgtttcctt tacagaggag cattgtggag
    21781 tcgaggattc tgtgagctat tatctgtatg cggttcctga aaaaccgatt gaagccttgt
    21841 ccttgtccga ctataaccat ctgcgtgaaa tcctggggct ttctcctgtt gtcatgggca
    21901 acaacgaatt tttggttcat tgtgatacat ggaactatat ggacggaatc cggcagggct
    21961 tgaagcagca gccggagatc acattaaatg gtcgaacctt gactattgca gagacaccaa
    22021 tcctgacgga gccgatggag cagtatcaaa tggccggaac gaagggatat gtacttgttc
    22081 tgccggatga agcagcttca caattagtcg gagaaaaaat ccgtctggcg atgaaactgg
    22141 aagatggcgg atacccggaa ttgaaaagcg acttgaagca attcctgaac agtgggaaat
    22201 ggcagccgga attgcagtcc ggtcaacagc tcccggaaaa agtaacgatg ggtgttacag
    22261 taaaggcgtg gggcgttgcc aattcgctga cgggatttac agctatttcg ttctgcggat
    22321 tgtatttaag cattattttc atcattctat cctgctcggt tcttgcattc gagcagcttt
    22381 ctgccataga caaaaaccag aagaattatg cagtcattga ccgtttgggt gtaccggggc
    22441 acaggcaagc ctccctgata cgcagggaac tgtccacagt ctttttgatc ccgctgctat
    22501 tccctctttt gctgacggtt ttgctgatag taggggcaca gttctttttc ggagaggcaa
    22561 tcttgcagca gggacttgta cctctttatg ggctggtgac gatcctgcta ttctgtgcaa
    22621 tctacctgac ctattttggt gcaacaatgt tcttattcaa acgggttatt cttcgaccgg
    22681 aaatgagata aaaattgaat tgctacaaaa atgaagcaat tcagcctgtt ttcttgtcaa
    22741 aaaagaaaat ggttaggaga tttctgcaac atttggtctt tattatagaa ttacaagtaa
    22801 gatagaaaat gtgattaaat ctgccgaacg acggcataag aaaaaaagcc gtcgtacagc
    22861 agacacattt ccacgcgcct atgaagtggc ggctttgaga aaatcagagc cgccgtttct
    22921 ttttgctttt tcgacgatgc tcgtgcgatc aggagggcgg tatataggag gataccgcca
    22981 tgtcaatttt cgtcattcac aatagccatg agttacacca gctaaaaaaa gtcttgcgcc
    23041 tcctccgggg agatacgact atctatcggc attatcattc caactaaagc agaagaatca
    23101 tagtcatttt tgttgcgcca gtttcccatt gcggggagct ggcgcttttt attgtcgatt
    23161 tttcgggaaa gctcccgaag tatgtcgctg ccgctcccca cctcctttcg attcactcgt
    23221 caatcgtccg tgaatcgaaa tggaggtaca taatgaagaa agtcaatctt agggacttat
    23281 atcccgatgt ttataaaact gatcaatttg tagaagtaac cgaagatgtg ctggagacaa
    23341 tccgagccgc tgagcgtgca gaaggtgcct atgaccggag gatgtatcgt tataaggcgc
    23401 actactccct ggactgcgac aacggcattg aaaatgcgat cctgttgaag ccccagacgc
    23461 cggaaatgct cctggaagaa aagcagctgc gggaacagct ctatgccgct gtgatggctc
    23521 tgccggagaa gcaggctaag cgaatctatg cccggtatta cctgggtatg cgtgtgagcg
    23581 agatagcagt agcggagggt gtagacccaa gccgtgtccg tggcagcatc cggcgcggtc
    23641 tgaagcagct ggcaaagtat ttttgaaaaa gtttcgtttg gtgcgctcgt tttttgctct
    23701 ttttgtcaga gttaatagaa ggataagttt tccccccttc aacagcactt tgacaatttc
    23761 atagacagca taacaggtac ataacccgtg tacgagcgag tgtggaacgc cgcgaagatg
    23821 gagcagccag ggaggggatg aacaaagctg tttcagagcg atctacgatt tcacaaagcg
    23881 gatggtggca gatccggcgc gaggactgca cgggggctta aagatacttc ctcacggcct
    23941 cctaaagact tggggggaac cctgcggcgc acgagtaaaa cgacgctgcg gagttatgac
    24001 aaccgcgcca gcggagacct gttatgttcc catcgtcagg gttgcgtggc aaatgtgacg
    24061 agaattgagt catattaaaa taggaagcga ttattttctt tgttggtcgt tttctattca
    24121 aaatcagata ccatgcgctg gcagttacgg ctgccagcgt attcatgtga ttttgaaagc
    24181 acgatgataa ccttgacaga ttggagggaa tatggtctat ggaaaagctc attacacgaa
    24241 aagaagctgc tgaaatctta ggaattagca tagcaacgct ggatgccgcc cgtaacaacg
    24301 gcttgatctc ttatgtgcag tatgttcaga atggctgtgt gtattttact gcggcaggtc
    24361 tccaggagta tattgcaaaa tgtacgcaca gggcaaagcc tgttgagaga agcacaactt
    24421 accgtaagcc tcgaaacgga agatcgtgag ccggtccgta tccttgatgg aatcagagat
    24481 gtttgataaa acaaaggtac agcgctggag actattctta ggaggtggag gaattggcaa
    24541 atagaaaaat aatgcttccc caatatggca cagttatgaa aagaggtgtc ctatattata
    24601 ggaccagaat caaag
//
LOCUS       DAWEJW010000033        24313 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_841_length_24313_cov_67.341207, whole genome shotgun sequence.
ACCESSION   DAWEJW010000033 DAWEJW010000000
VERSION     DAWEJW010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 24313)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24313)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24313
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_841_length_24313_cov_67.341207"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2751..2850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21801..21900
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6712 a         5081 c         6369 g         5951 t
ORIGIN      
        1 cgcacggcga cagtgacgtt acaggcagtc gaagcaccga ttcgtcccgg tgtctgcgca
       61 acagaagaaa acagagaata tccgcccgcc tttgtggatc ccggatttat tccggtgcgg
      121 aaactggaaa tgagcgctcc ctatatgaga ttgacaccgg agaagccgta tgtattgcta
      181 catacccgta tctatcccat ggaagccaca gaccgtaagc ttctctggag cattactgac
      241 gcttccggta tccccagccc tgtggcaaaa ctggaggaac tggaagacgg cgagagcatc
      301 cggatcacag gcgtcagtga tggcagtttt caggtaaggt gtatgacctg taacggaaca
      361 accaatatta aaatgatttc ccaacttgac tttgcagtgg agggcatggg acagacgttc
      421 cattctccgt atgagccggt gacgggtatt tcttatgtcg gaagttttgg cggagacgta
      481 agccgcggag tggagaacag tgccggaacg gacaaggcac agcctaccgg actggtctat
      541 gaagcgttag actttggtga attcggaagt gacgagatca cgctgtccat ttttgccaac
      601 gataatgatc cccaccggat tcagatatgg aagggagagc ccgaagagag cggttccggg
      661 acacagggaa cactggtggg tgaaataacc tatcagaagc cgggcatctg ggaagtcttc
      721 cagccggata ccttcgtatt gccccggaga ttaaagggtg taacaaaact gacaatagtg
      781 tcagaagata aaattttcct gaaagagttc catttcacca gacaggaaaa agcttatgca
      841 agactgagtg ctctcacgga cggagttacc tatggtgaca gctttgtgag aaccggggat
      901 gccgttgaac agatcggcaa taatgtctct ctcgaattta cggatatgaa ctttggagaa
      961 gcgggaaccg atagcattgt catctgcgga agaactcctt tacagcagaa tacgatccag
     1021 cttaggttta ccgatggagc tacacaggta cttcccttcc cgcacagcga ggaatatagg
     1081 gaactgcgtt tccctctgga aagagtgaca ggagagcaga atgtgacctt cgttttcctg
     1141 ccgggatgca attttgattt caaatggttc cggtttgaaa aaagcggatc tgaggagtaa
     1201 gtgataacta tgagcaaatg gaacagaaaa atatgggcag caatactggt ggtgggaatg
     1261 acagcgtctc tgactgcctg tggaggcagc aaaggagaaa acagcaatgc gtctgctgcc
     1321 ggtacacaga atgagactgc caccgtacag gatgagagca cggacagtaa cactgacagc
     1381 accggcagta ccacgtctct ggtgcaggac aaccgggtac tgcagatccc ggacgacaac
     1441 taccgtacct actatgagct tttcgtatat tccttctatg acagcgacgg tgatggcatt
     1501 ggagatctac agggcgtact ggaaaagctg gattatctga atgacggaga tgataccacg
     1561 gatatggatc tgggaatcaa tggtatctgg ctcatgccgg tgatgccctc cgtcacctat
     1621 cataaatacg ataccacgga ttatgagaat atcgatccgc agtacggcac tctggaaacg
     1681 tttcaggagt tgctgacggc ctgtcatgac cgtgggatcc gtgtgatcct ggatctggcg
     1741 atgaaccatt cgtccagtaa acatccctgg tttttgcagg ctgtagaata tctgaaaaat
     1801 ctgccggaag gcactgagcc tgactcctca gaatgtcctt atgtggatta ttaccatttt
     1861 tcaagggaag ccctgagcgg atatgcacag gtaaatggga cagactggta ttatgaagcg
     1921 agattctggg acggaatgcc ggatctgaat ctggagaatg aggcggtacg ccgggaattt
     1981 gagcagatcg cggacttctg gctggatatg ggtgtggatg gcttccgact ggatgcggtg
     2041 aaagagtatg ttaccggatc cacagaggac aatgtggaaa ttctaagctg gttcgcggat
     2101 tatgttcatg gcaaagatcc ggagaattat ctggtgtgtg aatgctggac ggatcagaat
     2161 acctatgcca aatattatga gagcggcgtg gacagcatgt ttgattttgc ttttgccgac
     2221 aagtccggta tcattgccaa tgtgatgaac ggtaaggcat cggcaacatc ttatgcaaag
     2281 aatctggaaa cagagcaggg catgtatgcg cagtacaatc cggattatat caatgctccc
     2341 ttctacacca atcatgatat gggacgcagt gcaggttact atgccgggga gaatagcgaa
     2401 gctcagacga aaatgggcaa tgcccttaat ctcttaatga atggcaatgt attcctctat
     2461 tatggggaag aactgggcat gaagggctcc ggcaaagacg agaacaaacg ggcccccatg
     2521 tactggagca aggacggcaa tgcggaaggc atgtgtaagg gcccggcaga tatggatgat
     2581 ttccggatga aatttgacag tctggaagaa cagcaggagg atcccgattc catctataat
     2641 tatgtgaaac aggtgatccg tgtccgcaac cagaacccgg tgatcgccag aggcaccacc
     2701 gcttatctgg agcagtattc cggtgaacaa tgctttgccc tgacaagaag nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaaacaggt ctgtgggcga accctacaga
     2881 cctgtatgct ttcttacaaa atcttcaaat tcgtttaccg gtaacggctt cgagaagtag
     2941 taaccctgaa tatcatcaca gtgaagattc ttcagaaact ctacctgatc cgaagtttct
     3001 accccctctg cggtaatagt cataccgaag ctctgcagca gctgcgtgat gtgcaacagc
     3061 agcttttcac cgttccggtc accaatgtaa tcgatcagac tcttatccag cttgattgta
     3121 tcgaaatgca tgacattcag cgaagacaga gaagaatatc cgcttccgaa atcatccagc
     3181 agtagagtaa atcccgcttc atggaactgc tcgatcaggg aggagatatc actgttatta
     3241 atggtagcac tctcggtgat ctccagcgga acatatttcg gatccagctg gaaagaattc
     3301 aaaagctccc ggtacttatc taccacattg ctgtagtata ggctgactct ggaaatattt
     3361 acagaaatcg gatacaattt tgctccagcc tgcagccatc tctgctgttg tgcgcacacc
     3421 gtgcggaaca catattcgtc cagtgttgtg atattaccgt ttttctcaaa taatggaatg
     3481 aacttgccgg gagagagcat ggtgccgtcg gggcgcttcc aacggatcag agcctccgct
     3541 cccagaatgt ttccggtgac ggggtctacc ttcggctgat accacagctg gaattctccg
     3601 ttcttcaggg catcttcgaa gacatcttcc atcagacgat cctcggagag ctgtttgata
     3661 tccagctcat cataaaaagc ataatgcctg gtacgttttc cttttatcgg atacatggcc
     3721 tgtacagcct tgccgtaatt ctgttcgatt tccttatgat cggcggtctc atagatgccg
     3781 cacagtggga acagtttcgg aatattaagc tttgtggaca aattggaaat atcctgacac
     3841 aggcactcca atctgggttc taaggtacac cggtcattat cccgcaggaa caggatgaac
     3901 cggtctgcat tgatgtgggc agaaaattca cccagatgtg taccggtttg caggatcttc
     3961 catacttcca acagtgtttc gtcacccttg gccacaccac aggtattatt gatcagcttg
     4021 aaatcctgaa gatccagggc aacatagaag ccactgtctc cgcctcggtg ggataatttc
     4081 tccttgaagc aggcaaaatt atcaccattc gtcagaggat ccacataggc cagacgatat
     4141 aactgtctgc gctgtatccg gatactgtgg acaaaagcaa aagtaccgcc tccggcaatc
     4201 acaatgatca gcaggatcag cagccgcacc tggagcaata tcggggcaga acgactggaa
     4261 agtacctgat cgggaacaat agtcaggacg aaccactgcc tgtcgctgcg gagattgtcc
     4321 attgctgccg catagtaata atacttctgg ctgtctccgt agaaatgacc accgctggaa
     4381 gaagaactct gaagcagcgt atgcagatcc tgcaagggct ggtcgttacg ggcatcctga
     4441 gtcagataat cggatatccg gtcggcttcc tgcagggaag ccggtgcatt ggaagatgtg
     4501 gcgatcaggt caccgtcctg atttaccaga cagctgaagc cctgattatt aaaggattgt
     4561 acgcttaaaa cttcttcaaa atgctggttc cgataggtgg caaataaaac accgataatg
     4621 gaatcactct gatcatacac aggaatgctg aatacattga tgggttccgg agtaccgagg
     4681 cgatcctgga gggtattggt aataccagga agtccctgca taccatcctt aaaaaaatct
     4741 ctgctggaca gatcctgtac atagccatcg gtgcttatca cctgaccgtc cggtgagacg
     4801 aaaccgatcc gtttaaactc ataggtggag acaaaagatc ccaactgtgc agcaatggat
     4861 tttgcattga ctgcatccgg ggaaacgctg atcgcatcgg caaggctgta taataagttg
     4921 aagttttcac ggagctcctg ctgtacgatc agggcgttct gcgtggcaac atcccggaga
     4981 gtggactgta cttcttcctc tgtatcctgt gtgatctggg cagaactcca cataactgtg
     5041 gaaattacca gcaccagagc aatgctcaga atgatcaagg cctgttttgg taatttactt
     5101 tttttcattt tatatcctct tatgtatgtt ataaaaatta ccttatataa tataccatta
     5161 tacacattta cgattgcatg gcaacaaaaa ctgcattgac agtccggatt tcctgtgcta
     5221 ctgtaagaat acgcaaatgc aatgaggaag agagtattta tcatagaagt tttacagaga
     5281 acattccgta aggctgagag gaatggaacg gacagataga gaagatggct tcggagcggc
     5341 gcaccgaacg gataccgggg agaataggaa cggtagaagc aaggctgcga caggatccgc
     5401 ctgttacagc ggatagggtt acggacccga agaggtctgt actgtgaggt acagacgaag
     5461 agaagtggta acacgggata acgctcgtct tcttgaaaaa cataatgttt tttgaggaga
     5521 cttttttatt gcaacaaggt cacaagtcaa aaattacgat gtgcttgcac aagagtaatt
     5581 ttatgacttg atgacctgcc ccgatatgag cataaaagtg gagcgtaagc ttcacgatat
     5641 gcgaatattg ggttggattg tgagagtgcg gagcacggaa cgatccgttg gaattaaaat
     5701 aaataagaaa aggagaatgt gatttatgga aagcaaagga aaatattatc ttaccacagc
     5761 gattgcctat acatctgcga agccccatat cggcaacaac tacgagatcg tactggctga
     5821 cagcatcgcg cgtttcaaga gaaaggaggg ctatgatgtc ttcttccaga ccggtactga
     5881 cgagcatgga cagaagatcg aactgaaggc tgaggcagcc ggcatcactc ccaaggaaca
     5941 tgtagataag atcgccggtg tcatccgcgg tctgtgtgat ctgctgaaca tttcctatga
     6001 taagttcatc cgtaccacgg atgaggatca tgagaagcag gtacagaaga tcttcaagcg
     6061 tctctatgat cagggagata tttacaaggg ggcctatgag ggaatgtact gtaccccatg
     6121 tgaatctttc tggacggagt cccagctggt agacggcaag tgtccggact gcggcggtgt
     6181 cgtaaagcct gctaaggagg aggcttattt cttccgcatg agcaaatatg cagacaaact
     6241 gatcaagcac attaatgagc atcccgagtt cattcagccc gtttcccgta agaacgagat
     6301 gatgaacaac ttcctgctcc ccggactgca ggatctgtgc gtatcccgta cttccttcac
     6361 ctgggggatt ccggtgacct tcgatcccaa gcatgtcatc tatgtatggc tggatgcgct
     6421 gaccaactac atcaccggta tcggctacga tgcagacggc aacagcaccg aacagtataa
     6481 gaagctgtgg cccgcagacc tgcacctgat cggcaaggat atcatccggt tccatacgat
     6541 ctactggcct attttcctga tggcactggg cgagcctctg ccgaagcagg tattcggaca
     6601 tccctggctt cttatgggcg gtggcaagat gagcaaatcc aagggaaatg tggtctatgc
     6661 ggatgatctg gtagattatt tcggcgtaga tgcagtacgt tactatgtac tgcatgagat
     6721 gccttttgaa aatgacggta atctgacctg ggaactggta gcagagcgta ccaattccga
     6781 tctggccaac accctgggca acctggtgaa ccgtaccatt tccatgagca acaagtattt
     6841 tggtggtgta gtagagaata ggaatgtaca gctgaccgag aacgatgcag cagtggatgc
     6901 cgatctgaaa cagactgtga ccggcactta tgcaaaagta gtagccaaaa tggaagaact
     6961 gcgagtagca gatgccctga ccgagatctt cggcatcttc aaacgctgta ataaatacat
     7021 cgatgagacc gaaccctggg tactggccaa ggacgaagcc aaggcagatc gtctggctac
     7081 cgttctgtac aatctggtgg aaggtattat cattggcgca tccctgttag agccttatat
     7141 gcccgaaacc gcagagaaga tcgcaaaaca gctgaatacc tccctgcgtg acttcgatca
     7201 gttagagcag ttcggcctgt atgagagcgg caacagggtg actgaccagc ctgagatcct
     7261 cttcgcccgt ctggatatga aggatgtagc aaaaaaagag gcagagctgg aagaagccat
     7321 gatcaaggca gcagcggagc atgaagcgga agaggcaaat gccgaggcag aaaaggcaga
     7381 gcaggaagcc atcgatattg agcctaagac agagatcgaa tatgacgact tcgcaaaaat
     7441 gcagttccag gtaggtgaga tcatttcctg tgaggcagtg cccaagtcca agaagctgct
     7501 gtgcagccag gtgaagatcg gcagtcaggt acgtcagatc gtctctggca tcaaggcaca
     7561 ttacagcccg gaggaaatgg tgggcaagaa ggttatggta ttggtgaacc tgaagcctgc
     7621 gaaactggca ggagtaatgt ccgagggcat gatcctgtgc gcagaagacg cggaaggcaa
     7681 tctggctctg gtcaccccgg agaagaatat gcctgccgga gcagagatct gttaaatagt
     7741 atatgacaac gcaactttac gaatgaggcg ggatgaactg ttactatttt acaaaaaaag
     7801 caatataggg cttaaaattt tataagaaat gatatataat gaataggacg ccaaagcaga
     7861 tgctgtaaaa ttgacatttg ttttgtgcgt cctatgattt ttccgtaacc aaggaggtct
     7921 tatgattaag gaagaatttt actttgattc ccgggacggc gaaagccgta tccatgcagt
     7981 gagatatact cccgatgacg gcaatgtaaa aggcattgtg caggtagtgc acggaatggc
     8041 agagtatgtg gagcgttatg agaacctggc tgaatttctg actgccagag gaatccttgt
     8101 taccggagaa gaccatctcg gacatggaaa atctgtggcg gaaggcggca cttacggtta
     8161 cttctgcgaa caggatccgg caacggtagt ggtaagggat gtacatcggc tgaagaagat
     8221 cacagaggaa agctatccgg aggtgcccta tgttattttg ggacacagta tgggttcctt
     8281 tattacgagg aattatctgt gtcgttacgg taagggagtc gacggggcgg taattgtcgg
     8341 aacgggaatg cagtccgggg gattgatcct ttgttccaaa gccatggcag gcatacagaa
     8401 gctgttctgt ggttccaggc aggtcagtca ttttattgat aaggctgcat tcgggaacta
     8461 caacaagcgg atcgatgtac ccagaaccgc aagtgactgg ctgagccgga atccggagaa
     8521 tgtggaccgc tatatagcgg atgagctgtg tggatttacc ttcactgtga acgggttcca
     8581 gacgctgttt gaactgatcc gcagactgca gaagcaggag aatctggaga aggtaccgca
     8641 gaacctgcca atccttatgg tatccggtgc cgaggaccca gtgggagatt acggtaaggg
     8701 tgtccggaag gcatgtgatt ctctgaagaa ggctggtgtg aaaaatatta ccgtcaaact
     8761 gtacgagaat gacagacatg agcttttgaa tgaggaagac ggtgaacagg ccatggagga
     8821 catctatcaa tggatccgca gggagattcc gggactggat taagaccgga aagctttcgg
     8881 atcagagagg cggtgcatca tgtcggatac ggcagcgggc acttacgaga tcagatgggc
     8941 ggaagacaaa gactggattc ctgccatgca tatgatctgg aagacatttt taaaatttga
     9001 ggcggtggat tatacagagg agggaatccg taacttttat gattttatta cggatgaaca
     9061 cctctataag tcgttccagc agggaagata tcagatgatg gtggcgctgg acggtgagag
     9121 agtcatcgga gcggcttccg tgcggaatta taatcattta tcacttcttt ttgttgatga
     9181 ggagtatcat catcggggaa tcggacggga tctgatgaga agaatgtgtg aatatctgaa
     9241 aaaagaggaa ggggaacggt atatgtcgct gaaagcagct ccgtatgccg tagagttcta
     9301 tcgaaaactg gggtttcatg cggtacatga agaggaggca ttttccggaa tacgtgtgac
     9361 ccccatggaa aaatttttat aaaacagaaa aggataagat ataaatgaaa atttttgatc
     9421 tggatagccc tttaatgaat gtactgaaca aaatggcaga tctgatgtgg ctcaatattc
     9481 tgacactgat ctgctgcatc cctgtcatta ccgctggagc tgcgctgact tccatgcatt
     9541 atgtggcatt gaagatcgtg cgtaacgagg agagttatat tacaagaagc ttttttaaat
     9601 cctttaagac caatttccgt caggcaaccc tgatctggtt gctgcttatg ctggtagcgg
     9661 cgatccttgg cggtgattac tacattatta ccaaatccgg aatgcagttc agccaggtgt
     9721 tggtggtact gatcatggca gcaggagtgc tggtgatctg cacttcttta tatgtatttc
     9781 cggtacttgc caaatttgac aatacaatca tgggaaccat tcgaaatgca tttatcatga
     9841 gtattttaca gctgcccaag acagttgtaa tgttcgtaat ggcatttttc ccgttgatta
     9901 tttatctggt atctttaaga ctgataccca ttattttcct gtttggtttt tcactgccgg
     9961 cttatgcctc tgccatgctg tacaataaat tcttccagaa gctggaagat cagttgttgt
    10021 cagaacagga gcctgcaccg gaaatctcgc cggaagagga tgagagagta ttccatgatg
    10081 aactggatga aagcctgaaa acagacgtga agtaacaaaa atgtcctgcg ggggcatcag
    10141 acgagaggtg ttcggagtat gcaaaagaga aaattttggg aaattttttt ccagtgggtc
    10201 tttccctgcg cattgctggt agttgcactg gttctgagta tttacgaatt taataataaa
    10261 atgaattcag ccatacggac ggtggtggat gatcaggcag agcagatcgg ctattactat
    10321 tccgcgggag tagatgacaa gctgggggca ctgggagata tcacgagtgc catggcaaac
    10381 attatggcga gccggccgga cagaagtgat gcttttgtat atgagaagct ggacaccatt
    10441 gtaaaagcca gcaacgccta tatgtctgct tattgtgctg tgaacggaaa cggaatgctg
    10501 agtgaccgcc gggagttcga tatgtcggag ttaaactatt acggcagcat cagcggaacc
    10561 tccgcccatt atatttatgc cggcacggat gggatcaacg gacagactgc ctttatctat
    10621 gtgtgtccga ttgcgatatc cggaaatgtc accggatatc tgctcagtta tatgaacccg
    10681 gataacatga aggaattctt cgacaattct gtatatggtg acaaggcatt tttctctctg
    10741 gtcaaccgga acggaaccat tatggcatgc tacggtgcca ctgacggaac ggctattttg
    10801 cagaatgatt tctggaattc cctgaaagat atttcggaaa gcctgggaag ctggaccttg
    10861 tttgaaagac agcagcagaa gggcaacaac ggcattttac atgttaagga agacggtgta
    10921 ggcaagatca tctgtcattt tccaatccgc ggtaccgagt ggaacctggt ggtcggtatt
    10981 aatgaatcct atctgtctgg aatccagcag tccttccgcg gcccgattgt gaatctgatc
    11041 gtcaagatca gtatttccat tgcagtagcg cttattatta tcaccgcaat caatattctg
    11101 gtacgcatca aagcctctga acacagtaag gtgttggagg ataaagcgga taccgaccta
    11161 ttgacggatc tgaacaataa gatggccaca gagcgtaaga tccgggaata catggaaaag
    11221 tacccggaca agcagggcgt attgttcgta ctggatgttg ataactttaa gaagatcaat
    11281 gatactatgg gacatgcctt cggagatgaa gtgctccgca acctggcagt acgtctgcaa
    11341 tccatgttcc gggctacgga tatcgtgggt cgtaccggtg gcgatgagtt catggtgttt
    11401 ctgaaggata tccgcgagat cagtatgatc gaacgggaag gcaagaagat cgaacagttc
    11461 ttccatcagt ttgaagtggg agaatatgta aaatattccg taacggccag cgtgggagca
    11521 gcggtcttcc ccggggacgg caagtctttc gaggaactgt acaaagccgc agataatgcg
    11581 ctgtatgtgt caaaaagaca tggcaaaaac cagcttagct tctataaaaa gccggaaaat
    11641 gagacaaagt agggcgattc ttgtggaata tgtccaagaa atgcgggcgg atttgttcaa
    11701 tctgacaagc atttcgaaaa aaaacaaact ttataagcaa ttcttacaat aggattcaaa
    11761 atctttgtga aatagtggta tggcacattt tcgtgatttt cgttatactg aattcagatt
    11821 caaacgagac atatcgtttc acaatgagag aaaataacat tggtaaatat gaaaggagta
    11881 ttttcaaatg gcaagtttat ctttaaagaa tgtctgcaaa gtatatccta acggtttcga
    11941 agctgttaag gatttcaacc ttgagatcgc agatcaggag ttcatcattt tcgtaggtcc
    12001 ttccggatgt ggtaagtcca ccacccttcg tatgatcgca ggtctggaag atatctcttc
    12061 cggtgaactg aagatcggtg acagagtagt taacgatgta gagcctaagg atagagatat
    12121 cgcaatggta ttccagaact acgctctgta tcctcatatg tccgtatacg acaacatggc
    12181 atttggtctg aagctgagaa aagttcccaa ggatcagatc gataagatgg ttaaggaagc
    12241 agctaagatc ctggatttga ctccgctgct tgatcgtaag cccaaggctc tgtccggtgg
    12301 tcagagacag cgtgttgcta tgggacgtgc aatcgttcgt aaccccaagg tattcctgat
    12361 ggatgagcct ctgtccaacc tggatgctaa gctgcgtgta cagatgcgta tcgagatcgc
    12421 taagctgcat cagagactgg gcaccaccat catctacgtt acacatgacc agactgaggc
    12481 tatgaccctt ggtaccagaa tcgttgttat gaaggacggc gttatccagc aggtagatac
    12541 tcctcagaac ctgtatgaga agccctgcaa cctgttcgta gcaggattca tgggatctcc
    12601 gcagatgaac ttcctggatg ctactgttaa ggtagaaggc gacatcgcta acctggtaat
    12661 cgcaggccac agcattcccc ttcctcccgc taagtccaag aagctgatcg atggcggtta
    12721 cgatggaaag gttgttacct tcggtatccg tcctgaggat gtatatgatt ccgagatgta
    12781 catcgagacc tctcctcaga gcgtatttac ctctaccatc aaggtttatg agttacttgg
    12841 tgcagaagta tatctgtact tcgatcttga cgagttccct atgaccgcaa gagtagattc
    12901 tcgtaccact gcaagacctg gcgataccgt taagttcgct ctggatgttg agaagatcca
    12961 cgtattcgac aaagagaccg agcaggttat taccaactag tcttaagttt cataagagat
    13021 ttcaaggtcc tgtctgtgaa tgcagacagg gcttttttta tgcattttgc ttgcacgagg
    13081 tgagtaaatc agatatacta agtatatata gttctgacat agatgaaaag cgagactgct
    13141 tgcaggtgga gcttttcgcc tatgaacaga acgaatggat atgagcaggg aggacgatag
    13201 tccggaatgc gaagagacgt tgcgaaatcg agaacgcgaa gagcgaagaa ttcgatatag
    13261 attatgctac agaaaggcgt ggacaatatg attacaggcc agattataca gacaagcatt
    13321 gatgaattaa aagcaatcac taaggtagac ctcggagtct atgatctgaa cggatccgaa
    13381 gtggcatcta ccatggaaaa ggatgacata accacagatc tgatcacagg ctttgcggct
    13441 tctccggcgg atagccaggt gatcggagta catcaccttc tgaagatccg tgacgaaggg
    13501 gatcttttat atgtactggt ggcacgggga atgacggatg acgtatatat ggtgggtaag
    13561 attgctgtca gtcagatcca gaatcttgtc attgcctaca aggaacggtt tgaccggaat
    13621 aatttctttc agaacctgtt gttggataat ctgttgctgg tagatattta caatcgggcg
    13681 aagaaacttc atgtggaagt cagctgtccc agagccgtat atctcattga gaccaaggat
    13741 gagaaagatg gcattgtatc tgaagtgctg aagggcatgt tttccccgca ggcgggagat
    13801 tatgtaacag cggtggatga gtccagcctg atcctgatca aatctgttga gagcaccacc
    13861 acacctgaga cactgcatga actggccgag actattgtag ccatgatgaa tgcggaggct
    13921 cttctggatg tgaaggtggc atatggcacc gtggtacagg aattgaagga cgtatccaag
    13981 tcttacaagg aagccaagat ggcactggat gtaggcaaga tcttctacgc agagaaaaag
    14041 gtgatcgcct acagcacgtt gggaatcgga cgtctgatct atcagctgcc ggtgaatctg
    14101 tgcaagatct ttattgagga gatcttcggt gacaatgtac ccatggatct ggatgaagag
    14161 accctgaaca ccctgaacaa gttcttcgaa aacaatctga atgtgtcgga gacttccaga
    14221 cagttgttcg tgcatcgtaa cactctggtg tatcgtattg agaagattca gaagagtacc
    14281 ggcctggatc tgcggagctt tgatgatgca ctgactttca agatcgcatt gatggtcgta
    14341 aattatatga aatatctgga cagccaggaa tattaaggac aacccctcgc ataaagttag
    14401 atagaaactt tatacggggg gattttatgt catatcggaa acagatcagc tatttggaat
    14461 tacaggaaaa cgggcaacgg atccggggag ccgggtatgc aaagctggaa agctgggaaa
    14521 aggaagagat tctgatggtg cagctgagag gacaggggat cgtgtctggc aaggagatga
    14581 tgctgctgta tctgcagcgg gaaagcggag aagaactgtt atgtgaaatg acggcagaac
    14641 gggggaccat ttcctgtgaa aaaagaattt ccagaaaagc ggattgggag ccgacactgg
    14701 ggatacgcat tcccctggaa cgtaattttg agatcatagg gcggtttgca ggagcggctg
    14761 gtgcgaagaa agtacctgcg cctgtgactg cgattgcacc ggttacggtg ggggaaatcg
    14821 aaggacaggg gcaaggtgga aaccagccgg agcaggcaaa gcggccgata cagacagaac
    14881 agcaggaaac tcaaaatagg gagatgcagc gggagcgggt gcaggagcaa ggcaggcaag
    14941 cccgtgaaca gaccgaaaaa gtggcagaaa ccgtagtcgc aaacgaaaaa atggtcagtg
    15001 gatcggaagc ccggagctcg aaaaaaactg ccacggaaca cattctccac atgaaagaca
    15061 ccaaatggga gcaattaagc agtatctacc cccacatcca tcccttccgg gatgccaggg
    15121 aatttctttc cgtgggtccg gaggatttcg ttgtgttgcg ggagcgttgc tatcagatga
    15181 cccacaacag ttttttgttg catggctatt ataattacag acatttatta ttaatgcgtc
    15241 aggaagtctc tggggaagtg aaatattaca taggagtccc gggtaatttt tatgaaagag
    15301 aaaaacaggt agccagaatg tttggctttg aaagttttga aggaaccaga gaaccggcat
    15361 gggaagggga tttcggatat tatctgatcg cagtggaact gtgagagaat cacagtcccc
    15421 gctcgtcaaa ttccggaata aagctttcac aggcggaact tccctcttgc cggaagccct
    15481 gttcaatgcc gattcgctcc gcaaagcgca ggacccggtc gtattcctcc ggtgtcactg
    15541 tgcgattgag tgcttcaata tctgccacct gcggaagagg tgtatattga ttcataatgc
    15601 taatatagat atgatccccg taggtctcat ggagataccg caggatcttc ttggagtctt
    15661 tcgcttgtcc cggcaggagc agatggcgta cgatcatccc ccgctgcatc agcccggtgt
    15721 cgggatccat gaccggatct aagacctgcc ggtacatttc cgcaatggca gcgcaggcaa
    15781 tctcaaaata gtccggggca tgcgaatacg cagcggaaag ctccggagaa aaatatttca
    15841 gatccggcag atagatatcc accagaccat ccagatggcg taaagcctcc ggagattcgt
    15901 aactgccggt attgtataca atggggatgg acagtccctg atttttggcc tgctgtaagg
    15961 caatgacgat ctggggaaga aaatgtgtgg gagtaacaag attgatattg ttagcaccct
    16021 gttcctgtaa ttgtagaaag attttcacca gatgttcggg agtgatgact cgtccggctt
    16081 tccccagagc gatgttatgg ttctggcaga agacacagcg caggttgcat ccggagaaaa
    16141 agacggtacc ggaaccggaa gtaccggaga tacagggctc ctcccaataa tgcagagagg
    16201 ctctggcagc cataatatct gccgtctggc cacaatatcc cacgccacca tccaggcgat
    16261 cggcatggca ggccctggga cacaggatgc agtcggacat gattttccgg aaccatgcgg
    16321 catcttgggg agccagatcg ccggaaacgg atgctatgct gttggaaacc gaaaaagggg
    16381 atgacatatc gcctgacaca atctataacc tcttaactga cttatccatg aattcacaga
    16441 acactttaaa tatcaaaacc gcacatgccg gaccaggata acagctccac ccagtttcct
    16501 cacggcacat ggaaggttct acttagtgct gctttgttcc caacctgaca cggttcgtag
    16561 attcccattg cgtaagaccg agtttcatca ctattcccat gataccggac atgtacggcc
    16621 tttttagtgt atcctgttcc ccggtttttg tcaagcctct ttgccttgaa ggaaacaggg
    16681 aagagtgtta taattaaaat aatatgtaat tttgtcgaaa gatagttgcc tacggagaag
    16741 gatccatgag aaacaagcga aaaagtatat ccctgctggg atggataata ggtgcacttt
    16801 tactgatata tctgggagta ttttgctacc tgaatctgtg taaatatgca caacatgtgg
    16861 attcggatat tgccgcagag gctttgctgg cccgggagat ctgggtggag aaggatatta
    16921 cacccaacga ctggatctcc agcacggaga gacggatcat tggaatgcct acggttgcag
    16981 ccgtatttta cggtatcacc ggcagcatgc aggcggcggc ggggattacc tgtgttctat
    17041 tgggtgcgat tttgctgggg acattttatt tttttctgag aaaattatct ttgagcagac
    17101 cggcatcaat tacggcattg ctggtattgt gtgcgctgcc aatcaacggt tttcgcaatg
    17161 aggggcagat ggttcctttt gtagcgctgc ttttgttcct atttgcagag tattatgtgt
    17221 ttcatggcat ttttctgttt ttcaatatcc tgttttacct gaaattaaaa gaaaaccggc
    17281 agatgacccg gaagaccttt ctggagtggc tggtattgtt tgttgccgca gttttgctga
    17341 actgtggagg acagagatgt ctgcaggtca tcatcctgcc actgcttgta gtggaagtga
    17401 tcgctttatt catggaatcc ggacatctcc ggcagaagct gcccggtgga cgttatcttg
    17461 ccacagccta tgtgggaagt cttgcacttg cttttctggt atcctgtctg tacggcggca
    17521 gaggagatta tgcagtctat ctgctgcagc cgggagaggc agtagagaaa ctgcttcttg
    17581 gggttccggc agccctgttg gagaactttg ggctggtggg aaatgcaaag gtagggagct
    17641 ttgcttctct gatgcagctt cttgtctggg tgttcctgat cctgttgggg tatggactct
    17701 ggtatgtgtt ccggaaaaat acagagagca cagacagaca aaaagatgca ctgacaattc
    17761 tccttgcttc tgtgggagtc acagcattta tcatcgggat caccagcgcg gaggcggccc
    17821 attattactt tttcatggca tggttcgcag cagctgttct ggtagcggtg atggtggatc
    17881 atatgtcgga aggacagccg gtgttcgctg gtctgatctt aacggccgta gcgctgttcg
    17941 cggtgctgaa tctgaaatac acctactgtg aagcggccac cacacaggat aacctgaagg
    18001 aatatcagga agtggcggat tatctgacag aagcgggaat cgactacgga tatgcagaat
    18061 tctgggatgc cgaacggatc tgtctgatca cggacggccg ggtgacgatg ggacattcct
    18121 ataccatggc cagtctttcc ggatactggt ggctcaccag tacgaaatgg tatcccccga
    18181 cgctgcccac ggaaatgcgg accgcttacg tcgtacggac agaaatgcgt gaagcctttg
    18241 aacagcaatt tccggaaggg gaagcaccga tactggaata tgagaatgaa aagctggcag
    18301 tatatgtcgg tgacagaaat tatgtggaac tataagataa aaatgaggga acattaatgg
    18361 aaaaaatata ccgtattctg ttgggaatat ttcttgtctg tctgattccc attcttttgg
    18421 tcctgagtta ctggtcctgt cggtattctt attacggaac ggtggattac agcatcagaa
    18481 atctcctgat aaaagacagt acctggaaac attttgttat tttcctgctg acatctgtcc
    18541 ttgtctatga gggagataaa tggctgaatg ctcaaaatca ggaaaaacag gaaaaaatct
    18601 gcatctatac attgttggca accagtgtta ctgcgtttct gctgggaagt atctacgttc
    18661 tcaacaatcc ctattatccg gtaggagatc agatcagcgc cacggcattt gcagcatatt
    18721 gccgggatgg caacttcatt atgttgtgct ccggaggcta tgtcgggatg tatcaacagc
    18781 aaaaagggct gggaatattg tatgagatat tgtttgccct gtttggaaat ttcaattata
    18841 ctccggcaaa aatcctgcat gtaatatggt gggtactggc gattctggca ggatacggtt
    18901 tcctgaaact gaatacagac agagccatat ttagaatcgt atattgtatt atgatgttgg
    18961 gctgtattcc ttttttgctg tatttaccgt atatttacgg ggacgtgctc agtatcagtt
    19021 ttggaatggt aatgttctgg gcggtgtctg cttatgaaca ttatgagaag aagaggtata
    19081 ttgcgttggc ggcatgtgtg gcagggattg ctgtacttgc ccggaaaaat acatggatca
    19141 tactcattgg agtgggaatt tatgcagttc tggtctgcct gaagaaaaag aagggacaat
    19201 atctgctggc agggtttgca attcttctga cggcagcact gacggtgaaa gcggtagatg
    19261 taatgtatga ataccgttcc ggatatccca gtgacattgg cattccgagt attctctgga
    19321 ttgcgatggg attacaggag acggatggca tggccggggt gtacaatcgc tatcagcaaa
    19381 ccacgtttgc agaacatgat tttcaacagg agcctgccgc acaggaagga aaggaatata
    19441 ttcgcgaacg attgcgggag ttccgggaga atccggctat ggccagagat ttctttaaga
    19501 gaaaactgga agatcaatgg atcgaaccgc tgttttccag cctcaaggca acagagtcgt
    19561 ttgatacgga tggagaaccg ctgtcctccg ggattaccag tctgtactat gggaacatac
    19621 atgaaaccgt atggaagctg gccaattatt accagagtat cgtatacttg gcaggacttg
    19681 tacttggaat tgctttgtgc ggaagatggt ggcaaaaaaa agaaattccg acagcattgt
    19741 ggctgccgtt gattggcata gtaggtggct tcctgttcag tatcatctgg gaagcgcaga
    19801 gcagatatgt atttccgtac tatgtatttt tgatcctctt tgtgccgatg ggactttatg
    19861 aaacgggaag agcgatttcc cgattgccga agcatagaag aaaaacaacc gaacagaacc
    19921 agacacagga agaaagctta cgagagatcg cctgatgcct ttctggcagc tttctcttgt
    19981 ttgttccgga tgcggagctc ttcaaaaaaa gtcttcagca tctggctgca ttccgtttcc
    20041 agaatgcccc gggtaacctt cacctgatgg ttaaactggg gcatttccag tatatttagg
    20101 atcgaacctg cacatcccgc cttgggattc atgcagccca tcactacttc agggatacgg
    20161 gcctgcacaa tggcaccggc gcacatctgg cagggttcca gggtcacata gagcgtgcat
    20221 tcttccagac gccagtcgcc gatcactttg ctggctttgt tgatcgctgt gatctctgcg
    20281 tgcgccaatg tattcttgtc tgtatttctc cggttgtaac cacgaccgat gatcttacct
    20341 tcatgtacaa tgacacagcc gatggggact tcacccaggg cataggcttt tttcgcctgc
    20401 ttcagagctt ccttcatata tttttcttcc acacttttga ttactgccat ggtatctgta
    20461 gcctttctgg cgcttgcact gtccgattta ttctgccttg gaaaaccttt tccaaaaaat
    20521 cacactatat ttcgtaaatg ctgtacattg acgaaactat cgttaactta tagtatagag
    20581 aatatatgag taaacgtcaa atggaacgag accgcatttt gctgcggtaa cggagagcaa
    20641 cgggcgcagg aactgcgacc ggaacggggg atttatggta tcgatcaaag aagttgcaaa
    20701 acatgccgga gtggctattt ccacagtatc caaggtctta aatggctatc ctaatgtcag
    20761 tgaagagacg aagaaaaagg tacaggacgc catcaaggag ttgaattata ttccgaattc
    20821 cattgctgcg gcgctttcct ccaagcagtt tggaagaatc gcgctggtgc tggatcccaa
    20881 tagacagacc caggccattg accaggtctt catgcagtat ctggtagggg cactggataa
    20941 ggccaaggaa ctgaacgtgg aagtcgtgac gatctttccg tccatgatcg cggggatgac
    21001 ggcggaagag atcacaagat cttttctgtc ccagagtatt tccggcgtgg tgatctatgg
    21061 catgtctaag gaggacaagg tgctgcagaa gctgatccgg gagaaacagt ttgcctgtgt
    21121 ggtagtggat gcgccgattg tcaattccag gactacatcc atcagcattg atcaggaaca
    21181 ggcccagtat gatgtggcga agaagaccgt gctggatgac aactgtaaac gagtgctcta
    21241 cattgcgggc agaagagacg gttatgtcac ggatcagaga ttgaagggga tgaagcgtct
    21301 ggtgaaggat ctggatctga ccatgatggt cagacaggga gacttcagtg agctgacggc
    21361 aagaaacgtg gcattgaaat atgcgaagaa taaggatgtg gtggtctgcg cttccgatct
    21421 gatggccata ggtgccatga atgcattgat cgatatggat attttccgtc cggtgtgcgg
    21481 cttcgatggt atcaccctga tgggatatgt cggcaagcag atgaatacca taaggcagga
    21541 cttctacagc atctccagca gagccgtgga agaggtacac cagctgatga acggtgggga
    21601 agggcatcag gtaatcatgc cccacagtat cgtgaaaatg tactataagg acattatctg
    21661 ttaagctatt ttgcacaaaa aatcaggaaa attaaagaaa aggttgcgga aaacgttttc
    21721 ctatgctatc ctgtgtattg taagagaacg acatacacaa aatctcagta ttgcgagggt
    21781 atgaaaagcc ggaaagtgca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21901 ctgagacaag gggatgggat accaaaaata agtatagtac ccagacaaag aaaaagaata
    21961 tgaaaaaatg agtgtggcac ggtacattca ttttggcaaa aataaaacta tcgtgcagaa
    22021 tggagtttga aatgaattta gagcagactt taaatgagat caccggcaaa ttatatggca
    22081 agaatatcgg tgcctgcacc gatgcacagc tgtacaccgg agtattgcag ctgaccaaag
    22141 agaagatggc agagactccc gccattacag gagacaagaa ggtatattat atttccatgg
    22201 agttcctgat cggtaagctg ttatccaaca atctgatcaa tttaggcatc tatgaggagt
    22261 tgagcgatat cctgaagaag aacggcaaaa ccatcgcaga catcgaagag gcagaacccg
    22321 aaccttctct gggtaacggg ggcttaggca gactggcagc ctgcttcctg gattccatcg
    22381 ctaccctggg acttcccggg gacggtatcg gtctgaatta ccacttcgga ttgttcaagc
    22441 aggtattcaa ggatcacctg cagaacgccg agaagaatga ctggatccag aaggattcct
    22501 ggctgaacaa taccggcaca aaattcgaag tatctttcgg tgaccgcaag gtgacttccg
    22561 tactttatga tattgatgtc gtaggttatg agaatggact gaacaagctg catttatttg
    22621 atgtcgagac cgtagacgag agtctggtaa agaagggcat cacttttgat aagaaagcta
    22681 ttgagaagaa cctgaccctg ttcctgtatc ctgatgattc cgatgaggca ggtaatctgc
    22741 tgcgtatcta tcaggaatac ttcatggtat gcaacggtgc ccagctgatc ctgaaggaag
    22801 taaaggaaaa gggttatgat ctgcatactc tgcctgagca tgtagcgatc cagatcaacg
    22861 atacccatcc taccatggtt atccccgagc tgatccgcat tctcaccaca gaggaaggtt
    22921 tcactatgga tgaagctatc gacgtagtaa gccacacctg tgcttatacg aaccacacca
    22981 ttctggcaga agctctggag aaatggcctc tggcttatat cgaggaagta gtgcctcagc
    23041 tggttcccat catcaaggag ctgagtgacc gtgtggcaaa gaaatataag gacgaaaaag
    23101 ttcagatcat tgacaaggac aagagagtgc acatggcaca tatcgacatc cactatggct
    23161 atagtgtcaa cggcgtggca gcgatccata ccgagatcct gaagcagagc gaactcaatc
    23221 atttttacaa gatctatccc gagaagttca acaacaagac caacggtatt accttccgca
    23281 gatggttgct gtcctgcaac catgagctgg ctgccttcct gacccagacc attggtgacg
    23341 gctacaagaa ggatgcggat gaattacaga agctgttaga gcataaggac gatcaggccg
    23401 tactggatgc catctacgac atcaagaaga ccaagaaggc agaactggta tcctatatca
    23461 aggagaagga gggcgtggag ctgaatcccg attccatctt cgatattcag gtaaagcgtc
    23521 tgcatgagta taagcgtcag cagatgaatg cactgtatat cattcacaaa tatttggaga
    23581 tcaagggcgg caggaaacct tctactccac tgacctttat cttcggtgca aaggctgcgc
    23641 ctgcgtatgt gattgctcag gatatcatcc atcttctgct ggtaatgtcc gacattatca
    23701 ataacgatcc tgaagtcagc ccttacatga agcttgttat ggtagagaac tataacgtaa
    23761 gctatgctga gaagctgatc cctgcctgcg atatctccga gcagatctcc ctggcatcca
    23821 aggaggcatc cggaaccggt aacatgaagt tcatgttaaa cggtgctgta acactgggta
    23881 ccagtgacgg tgcaaacgta gagatccatg agttggtagg cgatgacaat atctacatct
    23941 tcggtaagga tagtgacact gtgatcaagc attacgagaa ggcagattat gtctctaagg
    24001 actactacaa gaagagccct gtgatcaaag aggctgtaga tttcatcgtc agcaaagaag
    24061 tgctcaaggt cggcaaaaag gagaacctgg agcgtctgta caacgagctg ctgaacaaag
    24121 actggttcat gaccctgctt gacttagagg actacatcaa ggtaaaggat caggcatttg
    24181 ctgattacga ggatcagcag aagtggaaga agatgatgtt agtgaacatc gccaaggccg
    24241 gattcttctc ctccgacaga actattgcag agtacaacag agatatctgg aagctgaaat
    24301 aaatggaaag tta
//
LOCUS       DAWEJW010000034        23653 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_871_length_23653_cov_56.353123, whole genome shotgun sequence.
ACCESSION   DAWEJW010000034 DAWEJW010000000
VERSION     DAWEJW010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23653)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_871_length_23653_cov_56.353123"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2659..2758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7322..7421
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5653 a         6487 c         4801 g         6512 t
ORIGIN      
        1 gtctcctgca cggtcttaag tacccggtca aactgttcct tcacctgctc ctctggcacc
       61 tgttccatgg ctgccgccgg agtaccggta cgtttggaat aaataaaggt aaacgcattg
      121 tcatatttca cacggcggat cacatcaatg gtctcatcca cgtcttcttt cgtctctccc
      181 ggaaagccta cgataatatc tgtggtaata gccatatccg ggatctccct gcggatcttc
      241 tccgccaact ccagatactg ttccttggta taacgacggt tcatggcttt taaaatcttc
      301 gtggaaccgg actgtaaggg cagatgcaga tgtctgcaga tctttttcga atgcttcatt
      361 acctcaatga gttcctccga cagatccttg ggatgggaag tcatgaatcg gatccgctcc
      421 aggccttcga tcttctccac ttcctgcaga agctgcgcaa aggtaatggg gtcctccaga
      481 ttcttgccgt aggaattcac attctgtccg agcagcatga tctccaccac accgtccgcc
      541 accaggcctt caatctcccg aatgatatcc ttcggattac ggctgcgttc cctgccgcgg
      601 acatagggga caatacaata gctgcagaaa ttattgcagc cgaacataat attgataccg
      661 gatttgaaag aatattttct ctctaccggc agatcttcca cgatcttgtc cgtatccttc
      721 cagatatcaa tgaccattcc ctcattttca aacagggagc ataacagctc tgcaaatttg
      781 aaaatattat gcgttccgaa gatcaaatca acaaaacgat aactggtctt gatcttttcg
      841 atcacggaag gttcctgcat catgcagccg cacagggcga tcttcatatg gggatttttc
      901 ttcttgtaac cgttcagtac ccccagtctt ccgtaaacgc gctgatttgc attgtctctg
      961 accgtacagg tattgtagat cacgaaatcc gcattttcgt cctcaatgat ctcatagccc
     1021 actttctcca gaattcccac cagtttctcg gaatccctgg cgttcatctg acacccgaaa
     1081 gtcgtcacgc agcaggtcgg ctttctgccc agcttttctc caagctttgc cacatgaccg
     1141 gaggccttct ccataaaata atactgtctc tccgtttcgt tcatttgtgt tacattcgtc
     1201 atataaaaaa atgttgcctt tccaaatcag tttttgttct catcttatcg attcgcatat
     1261 tttgctgcaa tggcgatcag cagttccgta gtcttctcca tggactgtac gcaggcgtac
     1321 tcgtatctgc cgtggtaatt ctctccacct gtgcacaggt tcggacaggg cagccccata
     1381 aaggacagtc tggctccatc tgtaccgccg cggatcggag atactttggg ctcgatcccc
     1441 agttcttcca tggcagcttt cgcgttgtcg atcagatgca tgtgctgctc catgatctcc
     1501 cgcatgttat aataagaatc tttcacatct accacaacag tgccttcccc atacttccgg
     1561 ttcaggaaat cggcgcagga cataaacagc tctttttttt gttcgaacag ctgtctgtta
     1621 tgatctcgga taatgtaatc cgccgtcact tcctccacgt tgccctgtat ggcgtccagg
     1681 tggaaaaaac cttcatatcc ttccgtatac atcgggttct gctcagtggg cagttcactc
     1741 tggaactcct gtgcgatcag aatagcattt ttcattttcc ccttggcgga accaggatgt
     1801 acactcctgc catagacatg gagttttgct ccggctgcat taaagttttc atattccaat
     1861 tcccccagcg ctccgccgtc cacggtgtag gcataatccg caccgaacag cttcacatca
     1921 aaatgatcgg ctccggcacc cacttcctca tccggggtaa agccgatccg gatcttacca
     1981 tgctttttct ccggatgcat taataaagtc tccgccatgg tcatgatctc agccacaccg
     2041 gccttgtcgt ccgcgccaag caaagtcgtt ccgtccgtca cgatcagatc ttgtcccata
     2101 taatgtaaca gttccggaaa atcctccacc ttcatgacaa tattctctgc tgcattcaac
     2161 acaatatctt ttccatcgta attctctacg atccggggat ttacgttggt atccgtcacc
     2221 gccggagagg tatccatatg ggaaatgaag ccaagtaccg gagctttctc gcagcctgcg
     2281 gatgccggaa ttgtggcata gacataacag tgctccttgt cataagtgat ctctgcggcc
     2341 cccatggcct ccagttcgct caccagctgt ctggccagat catgttgttt catggtactg
     2401 gggatggtgg tgctttcttc actggactgg gtatcgatct ttacatatcg taaaaaattt
     2461 tcaatggtct tgtccataat ctcactcctt aacttataca ccatctcgct taaaagctcg
     2521 atgatgtaca ttcgccaaat acagatcctt gtgcaagcac gaacctgtac ctggctcatc
     2581 gtatacacaa atacaactgt cctctcttac gctcttataa acgcggacag ctgtatctta
     2641 tttatcatat catggtgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     2761 atccgaaaaa tcccacactg cttccagcga gcagaccgct cccaaaaaag tgaccagtcc
     2821 atagcccagg cgataccact gggttcggtc acagccgccc gtcagatact caaaagcttt
     2881 ctccccctga tattcccagg cgataatcgt ggcaaatgca aacagtacaa tggagattgt
     2941 gagcatccat tcccctgttc tgccgaaagt atctgaaaaa acagcgatca tgagggctgt
     3001 accgttcagg acttctcccc gggggtccct ctgccccagc attccactgg ccgcaatggc
     3061 aagccccgtc agagagcaga tcacaatggt atcaataaag acacccgtca tgctgatata
     3121 tccctgatgc accgcatctg acgtatctgc ggcagcagca ctgattcccg ccgcccccag
     3181 tcccgcttca tttgagaaaa cacctctgga gaccccatag cgcatcgcca ttcttccgga
     3241 atgggcgatt ccgttttcca ctgcaagcat cgtccctgcc gcccctcctg tcatagcctc
     3301 cgggcagaaa gctccccaca ggatctgcac cacaccggcg ggaagattct taaaatgggt
     3361 aaagatcacc attcccgttc caaacagata aaacactgcc atacagggca ccagatattc
     3421 cgtcaccttc gcaatggttc ctatccctcc cagaatcacc agaatcgtaa gcagacttaa
     3481 cacaattccc gtgactgtct gttttacctg aaacgtcacc gaaagtgcct ccgcaataga
     3541 atttccctgt gtcatatttc ccatgccgaa ggaagccagc acagcaaatg ctgcaaacag
     3601 catggcgagg atcctgcctg ccgttttctg tgggaatgca ttttgcagta catacatcgg
     3661 accgcctgcc ggttccccct tttggttttt gacacgatac ctgacacaca gtgtgctttc
     3721 taccagtttg gtcgacagcc cgatgatccc ggacagtaac atccagaaca gagcacccgg
     3781 ccctcccagt accatggcag tggctacccc tacaatattt cccgttccaa tggtggccgc
     3841 cagttccgtg gtcagggaag aaaacgaaga tactcccctg ccctctgttc ccttccggct
     3901 ttctggcaaa aacacacgcc tgagtgcagc aggcagattc ttcaagggaa gaaatcgtaa
     3961 gctaagcatc agataacagc cggttcccag caacaaaaaa ataagccagg gtccccatat
     4021 gatccggtct gcctgctgta aaagtttttt tattcctgtg atcataaaca gtcgtatctc
     4081 cccgacttat tttctaaaat atattcttta tttttctatt ttataccgtt gctatcactg
     4141 tccggctgct tttccggatt cctgtatttt ccggaatgta tttactggat atccggcaga
     4201 aaacccggta tcttttctgc gatctcacgg atcccgaaca gatatccgat gcctttctcc
     4261 agttgtcctt cggaagcatt gagcagtgtt gcggtcagat gcagcttatg ctctccatat
     4321 gcaagatcac acgaatactc accgttgtcg ttttccatgg tcatccggtc ctccgcttct
     4381 atttcagctt ctttatccca catgatctgt gcattggtat cataaggtgc ctctccttcg
     4441 atttcaatat aggtcccgga atcataatca tgcagataag actgcatata atcggtgata
     4501 aaataataag gggcatccgc accaatatca tcccaggtga caggttctgt ctcatcttcc
     4561 aatacaacct tcatggcata gagtgttccg gtcacagaat ccacgacaca ctctatactg
     4621 actcccgtaa cctcctcttc cttcatgcgc atacagatgt cataggccat gatcgcgact
     4681 ccgtccgaca gcttttcatc atacaaaata tatcgtttga tggaaatatg atctgtatcc
     4741 atatttttga aaaaatagtc ctccgggatc acacccatat cctgaaaagt gtaattccat
     4801 tccgtattta aaatatattc atcgatcaaa ctatatattt caccatccgt tgcagcttca
     4861 taatcctgtt cagagatata gtactgtttt cccaaagcga taccgtctgc cagattttga
     4921 agtctgcgca cggtatctgt ctcatactct tccttccaga cggtataatc cgttcctgta
     4981 tttgatatac tgactgaccc agtcagcctg ctcttatcct gcaatcgtaa cagcccttcc
     5041 ggtcctgcaa atgcaaatat gatcagcacg agcagtccgc ccatacatag taggaaattg
     5101 cgcctgttct tattctcctg ttttttcata ttccgccttt tgctcccctg ttgcttttat
     5161 tttttacagc atgtatttcc ccgggaaaat ataaccgcat taccgtgcct tctccaagct
     5221 tgctgtcaat cttcagttcc ccctgatgca gggtaatgat cttctgacag agagccattc
     5281 cgataccggc tccgccctcc ttgcgggatc tggacttatc caccatataa aaagcctccg
     5341 tgatccttgc gatctcttcc tgagggatcc ccctgccgtt gtctaccacc ttgatctcat
     5401 atccctgtgt gcgcttactt cccttcatca gaataaatcc gccttcttcc acagccttta
     5461 cggcattatc caacagatta tataacagtg acagaagcag atccctgtcc ccccagatca
     5521 ctcctctttc cagatcgatc ttcccccgga tccttttacg tccaaagggg atccgcatcg
     5581 tatcccgaat attttcctcc agttctttcg tctgaagctt cttgaattct aattcctgtc
     5641 tatccattcc caccagctcc agcaatttgt gagagaggct ttccagacgt tttccctgac
     5701 tgaaaatata ataggtggct tcgccccgct cctgttctgt cagttcatag gagttcagca
     5761 tatccgcata tccgatgata gaagtcagcg gtgtcttgag ttcatgggca aatgccgccg
     5821 taaaatcttc cttctgtttt gcttccagct ctttttcctc catctgacgt accagtctgt
     5881 ccgtcatccg gttaaagctc ctggacagtt ctccgatctc atctgtgccc tgatagacag
     5941 ctctcttctc aaaatcccct cctgcaatcc ttcccgcagt gcggtttaac tgctctatgg
     6001 ggcgggtcag atatctggac agcaaaaaag cagccagacc accgatcacc agcagacatg
     6061 ccagagcaat accatattgc cgcatcatac tactccgttc ttcataaatg ccgctcagat
     6121 ccttcaggat ccctagatat accgtatctg ccgaatccgt cgcctgatct gtgataatgc
     6181 tgagcagata ataacacgtt cctaccttgc ggacaccgta gacctgatct gcaccgggat
     6241 ccatagtccg gagcatttcc tccgcaagcc tcctgatctc atcctgcggc agcttcaggc
     6301 tgccctccag agtgatcgtg ccatctgttt caaggatcat acattccgta ccgctctgac
     6361 cggtcatccg ttctgttgcg gaatttcctg cctgtatccc agcaaattcc gctccgtatt
     6421 tttccatgtt ctgatacacc atatcatata tggtctgaaa caaaagattt tcccgttttc
     6481 cctgttctgt ctcccgttcc agcatccggt caaaatagga agacagcatg aaagttccaa
     6541 agagcagaaa aatcactgta agaagcgcaa tcactgaaaa aaacaattta tgagcgaact
     6601 tcattcaacc gataccccac tttatatact gccacgatca tctcttccca gtgcagtttt
     6661 ttcttcagcc gctgtacatg caggtccacc gtgcggctct gtcccatata ttcaccgccc
     6721 catacggtct cgtatatagt ctcccggtac aaagccctgt ttttatttct tgcaaacaat
     6781 aacagaagat caaattcctt cggtgtcaga tagatttctt ctccatccag tgtcactctg
     6841 tgggatgccg tatctatagt tacattgttg acctgtatca tcgtctccgt cttatgatat
     6901 ctgcgcagca ctgcctctac tctggccaga agctccacga tctcgaaagg cttcggcaga
     6961 taatcatctg cgcccatgcg aagccctttt actttatgtt ccgtagaacc caaggctgtc
     7021 agaaagatca ccggaagctc acaggctttc gcataatcca tcagttcata accattgatt
     7081 cccggcagca tgatatccaa aagtgccaga tcgaatttct cccgctccat aagatccgct
     7141 gcggtctgtc cgtccggtgc ccaggtgcac tggtacccgg ctctcgtcag attgatccgg
     7201 atcagattgg caatgggctc ctcatcttct gcaattaata ttctaagcat ttccctgtcc
     7261 ctcgaccacg cttcccaagc tcacgaattc gtgctatcgc actctgcatc ctgcttcgca
     7321 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntattatagc atatctcatc
     7441 tttgtatcca atttgtatca tctatttatt tggcactatt ttttcgtccg atcataccat
     7501 atattaaatg aattattgga aaggattgct atgaaagact attcttttga agaacatttt
     7561 gaaaaatatc cactggccat ggcctatgtt ccctggcagc atttcaccaa aatgtatgag
     7621 aatctggaaa aaggctacca ggcgggtacc atttttccgg agcttgacaa accttttacc
     7681 ggaaggagat gcgtaaaatg aacagtgaag aaagaatgct gcacgacatc ggcattgccg
     7741 acttcgttct gacggatctg atgctgtatc tggacactca ccccacagac cggaatgcca
     7801 tggagtattt caaccattat accaggatca agacacagat ggagcgggaa ttctccagag
     7861 aatattaccc cctgcggaaa gatctggcag aaagtaacaa ggagtggcgt tggggcaacg
     7921 ctcccttacc ctgggaagga ggatgtaact gatgtggagt tatgagaaaa gattacagtt
     7981 ccctgtaaat atcaaggaac ccaatgccaa actggcccag gtcatcatct cccagtacgg
     8041 tggccccgac ggggaaatgg gtgccagcat gcgttatctg tcccagcgtt attccatgcc
     8101 ctttaaggaa gtggcaggac tgttgacgga tatcggaact gaagagcttg gacatttgga
     8161 aatggttgcc accatcgttc accagctgac ccgtgacctc tccatggaag agatcgagaa
     8221 aagcggattt gccaattatt atgtggatca caccattggt gtctggcctc aggcagcagg
     8281 cggtgtgccc ttcaatgcct gtgaatttca ggtaaaagga gacatcatca cagatctgca
     8341 cgaaagtatg gctgcggaac agaaagcccg ctccacctac gacaacattc tgcgcctgat
     8401 caaggatccg gatgtctgtg aacccatcaa gttcctcaga gcccgggaga tcgtccacta
     8461 ccagcgcttc ggtgaagcct tgcgcattgc ccaggatcac ctgaactcca ggaacttcta
     8521 tgcgtttaac cccggttttg accgctgcga ttcctgcgag acctgataac tgtttaccat
     8581 gcacgcttcc agaatccaat tttgtatcaa aacaatttag aaaaggctgc cggttccgaa
     8641 tcctataatt tccgtttcga aactggcagc cttttttcaa ttttcagaat tatctctttt
     8701 cgccaggtat tatctctctt cacccatgaa gaacaatgcc agtgttccgg ggccggaatg
     8761 agaaccgatg gtggggccca catagttgat gaggaagctg tccatgccaa agcgcgcctt
     8821 taccaagtct ctcaccagtt ccgccgcagg cagatcatca ccgtggctga tgaaaacaat
     8881 atcgttctgg tcacggtagg atcccatctt ctcctccatg taatccacca gtgcatgcag
     8941 agatttctta cgtcctctta ccttaccgat gacaatcaga tgtccatcgt tatctacatg
     9001 aagcttgggt ttgataccgg ccagtgttcc tacaaatgcc gtagccttgc tgacacgtcc
     9061 gccgcggtag aggtgataca gatcatccac ggtaaataca tgtaccagat gcagacgatt
     9121 attcttcacc cactctgcca cttcttccat ggatttgccc tgatctctca aagtcactgc
     9181 cttgtggacc agcagtccct ctcccagaga agcgcacaga ctgtcgatca cgatgatctt
     9241 gcaatcgagc tgttcctcca tgatctcatc cgccgcgatc cgcatgctgt tgtaagtacc
     9301 gctcagtccg gaggaaaaag cgatacagag gatctcttta ttttctttca gatattccgt
     9361 aaaatgagcc tttgcctcct cagggttgac ctgggatgta gtcggcatct tgccctgtct
     9421 catcaatgca tagaattctt tttcgggaag ttcctgtcct tgtccataag tcacaccatc
     9481 catgatatag ctaaggttca tgcatccaag atcatgttcc ttcagatact ctaccggcag
     9541 atcagcagta ctgtctgtaa cgattttaaa cattggttac cctcctgatg ctatcaattt
     9601 ttcctaagca tacatcaaaa gggcacccat ttcaattttc gctgacaaaa gtgatacatt
     9661 ttgtcacatt tttctagttc ctggcattct tccacggaag aaacgatttc tcatttttgc
     9721 tctgtcaaaa ggaatcagcg gatacagata gctttgtccg gagattgtct tattcgtaag
     9781 gagcgccagc gcaaatataa tcagtccggc aatataaccc catattccga aaagctgtgt
     9841 caaaatcaga ttgaggatcc gcataaattt caaggcataa cccaattcat aattagcctg
     9901 cgtgtaatta gcaatggcca caaatgccat ggcaagcata acctcactgg agaaccaccc
     9961 actctccacc gaaaattctc ccagcaccag cgccgccatg acactcaagg gagtactcaa
    10021 catattcggt gtgttgaccg ccgccagccg cagtccgtca atggccagct ccaacagtaa
    10081 aaactgcagg atcagaggca cattgggatc ctctttcaga ataataaatt ccagattctc
    10141 tgggatccac atccgatgat tcataaggag cagatatgtg ggtgtcacga aatacgtgag
    10201 cagtgcgatc aggaaacggg tcagacgcag atacgttccg gtgaccggcg gaaagtaata
    10261 atcatccgcc tcttccacca catcaaagat cgtggtgggc aggatcatgg cagaagggga
    10321 attgtccacc aggatcacca gattgccttc cagcacctgg gccgccgccg tatccggccg
    10381 ctccgtgtat ttgaacttcg gaaacggatt gtaccacttc cgctggtaca gacattccgc
    10441 caggctttcc tgattcatgg ccagagcatc cacctgaatg gaacggatcc gttcccggat
    10501 ctgcgtaaga agtttcggat ccacacggct ctccatataa cacagtacga tatctgttct
    10561 ggaattctcc ccggcattaa gcatttccat atgaagatcc gtactacgga tcctgcggcg
    10621 gatcaatgct gtattgaata ccactgtctc cacgaagccg tccttggacc cccggagtac
    10681 cttgtccttg tccggctcct ccacccctct ggcgggatag gttctggcat cgatcaacac
    10741 cgccttttca taaccgtcca ataacaggac aaagacgccg gacagcaaag ctcccagtat
    10801 tttctcccag gtatcgtcca gatccacctc cacgtaggga atcgcgattt tggacatctc
    10861 atgggcatcc tttgggaagt cctccgcctt cagatccatc agatactgta atattttctg
    10921 catcagttca tccttgcaga aaccatcgat cagatacatg caggcatcgg tgtcaccgag
    10981 acggatcacc cggtgaataa tatcgaaatt ggtttccagt cccatcagtt catccacata
    11041 ctgcatattc tgctgaagct tttccgatat tctgacgagc tttggttgtt tgttctgtgg
    11101 cataaaaaat cccccttggg tctatgatct aaatttcatc atgtcccatt cgggggattt
    11161 tattctgcta ttttgttgtt ttacctgtta cttcgtggtg cttccgaagc cgccgttacg
    11221 gattccttcc gcatcatcat cttccgtgat gccatactgc ataaagattc cctgtgcaaa
    11281 tccggttccc gcaggaacct ccacggtctt gccttcattg gaatcattgg tgatcttcat
    11341 gaagatatgt ccttcattat ctgaaaagaa atagtcactg tcaatgatgc ccacggtatt
    11401 gttcatctgc aaacggtatt taaagcccag tccgcttctc ggataaatct gtaataccca
    11461 gtcctccttc atttcagcac gaatgccggt aggaatcttg atggtctctc ccggggccag
    11521 ggtaaaggca aacggtgcga agaaatcata gcctgcgctt cctttcgtgg ctctcctggg
    11581 aagttttaaa ttttcataca tttccttgat ctgtgcttcc gtatactgag gatatccctc
    11641 ctgcatggca gtcaaaaact gccccggact tacaaaatga aattttgcta ttctgtgcat
    11701 tgtctgttct ttcctttttc ttcttacatt accggctgaa accagccgct tcctatgcgt
    11761 aatccccgca gtgcaatacc ggcacccgtc tatggtcact ggccagggtc ttcttcacat
    11821 cgatcacccg ctggttctta ctgcccttcc acaggagctt tacatctttt tcctccacat
    11881 ggaattcacc gtctactacc acatccacat actgcatcac cggcagatca cagatgtctt
    11941 cccagctgtc cccggtgtac atccagatcg ttttcgtggg atatttttcc cggatttcac
    12001 tcatgagttc tctgacatcc gcccggttca cgggatgcat cggatctccg ccggaaaagg
    12061 taataccgga aatgtacggc ttttccaact gttcgaagat ctcctgtttt gcgctttcgt
    12121 caaataagag tcctccgtca ggatcccagg taataggatt ctggcattcc ttgcagcaat
    12181 ggttgcagcc tgccacccac agtacgaccc gcagaccatc gccgttcagc atatcgtcct
    12241 tggttatatt atgatatctc atttatctgt ccatcctgtc tctacatact tttccgctct
    12301 gcgatctccg ccatcttggc atcgttcagt cttgtatctc catgtactct ggagtaagac
    12361 agatagccgt tcatacggtc gatcttggtc agattccggc tgccgcagac aggacataca
    12421 tccatttcca gttcctgatg gccacagtcg tcgcagtaag ccagagacag attgacaccc
    12481 tcgtagaagc ccatatccat ggctctgtgg atcagtgtct tgatggcttc ggtattgtaa
    12541 tcaatgggat attttacata ctggatctta ccgccgttgc tcagatccca gaaacggttc
    12601 tccagatcct gtttctggat cggcgtaata tcctctgtca catggcaatg gaagctgttg
    12661 ctgacatatt ctcggtcgga tacaccctcg ataataccgt atttttcacg gaactgcttg
    12721 acctgaagac cgcacagatt ctccgctgga gttccgtaga tggcatacag attgccgtcc
    12781 tcttccttgt actcggagat cttctggttg atatgctcca gaacttctac cgcaaatgca
    12841 ccgtcttcca ccagtgattt gccgttgtac agttcctgca gttcatttaa tgccgtaatg
    12901 ccgaagctgg ccgtagcgga tttcagtaac ggtttgatct tgtctgtcag cttcagatgt
    12961 ccacccagga aaccgccctc gcaatatgcc agaggattcg tggacgcacg catttctccc
    13021 agataagcat aggtccggat atgcagctga cggatcagtt ccagataata atccagcact
    13081 tcataaaagt ctttgctttc ttttcttgcc ttggccagaa tcatgggcag atgcagggat
    13141 accgcaccga tgttgaaacg tcccacaaat accggctggt ccttgtcatc tgcaggatgc
    13201 atgccgcctc tctcatacca tggagacaga aatgctctgc atcccatggg agatacgatc
    13261 ttgccatact gcttgtacat gcttgccaca tagcccttgc cggtaagact cagccagtcg
    13321 ggatacatgg tcttggcaga gcattccaca cctgcctcga aaacatcctc cagcggcttt
    13381 ccggggccgt gcagattctc atcatataag aatacgatct tagggaacaa tacaggcttc
    13441 ttgtgatcct ttttgccctg tccctttctg cggacatcca gcatggtaat agtcgccatc
    13501 tttgcaaatt taccggtgcc ggttcctgcg gtcacggtaa taaacggata atcaccacgg
    13561 ctgctggcca cagtattgaa cttgtactcc cacccctgga agccctgttc aaattctttc
    13621 tggacatctg ccagtgcctc tgcttctgcc acatctgcgg caatacccag acgctcatat
    13681 tttgccagcg ctctgttata agacttctcc gcatacggct ccaggatcag atccacgctg
    13741 ggtacggtga atccgccgta ctgctggctg gctgcactta aaacgatatc gccgataaca
    13801 tcaaacgcca catccagtgt cttcggctca ttgtaccaga tattgcccat ctcaaatccg
    13861 ccggtcagta cactctgcac gtcgaacaga caacaattca tggtatctct tctggcggac
    13921 atgtcatgga cataaatata accgtcccgg caggcctgga tctcttccgt ggtcaggaag
    13981 aacttctggt acagttcctt gttcagctga ttgaagatca ggctgcgctt ggtggatacc
    14041 agtgcggaat ccgtgttggc attttccttg tctccgatgt acatgatgga ctggctcttt
    14101 ttgtagacat catccagcat ccgtacaaaa tcctgcttat agttacgata atcgcggtag
    14161 ctttttgcga cgaccggttt tacctgctct aaggcacttt ccacaatatt gtgcataatg
    14221 ggaatgggta cttcatccac ttccagttca tttacttttt ctaccacata gcggcagatc
    14281 tgctcttttt cttctctggt gaattttgtc agggcacggt acgcactctt gcctacagcg
    14341 ctgatcacct tctgtacatt aaaaagctct ttgctcccgt cctttttaac gacgtagatc
    14401 tctctgtccg gtctgaactt tttgccataa tcttcatttt cttcatgggc atgttcaaaa
    14461 ttactgctca tctttctctt cccttcatcc gataatcccc ggctgttgcc cggatttact
    14521 gtattcccct cacttacgat aacgtttgta gataccatat atggtatccg tagtcatcaa
    14581 aaagcaatat attgtgggtg ctatgtacca gtataaaaaa gaatccctgg cttgtcaacg
    14641 agggatccct tagaaaacta tacaaaaaaa atttcaaatt ttaggtaact ttttattccg
    14701 cctcctgtga ctgttcccgt aaaatatctg aaagccactg tacatagcgc ttttgtaccg
    14761 cttccggcgc tacgatacgg gccccctgtc cgataccggc cagccatccg aagaactgtc
    14821 cgctgaccgc cacctttgcc cggatccgga acacttcctc cgacatggga cggatatccg
    14881 tctcccttcc gaagcggtcc agtaccacgc cggtcaggcg caccggaaac tccatggtca
    14941 ccacgctctc ctctccggca aacatgccga aggtctggtt cgtataggag gtcacatcga
    15001 tctgtgaaaa ctgttccact ccttctctgg gcagatccgt cacttccgcc tgtcccatct
    15061 tgtccacacg gtaatgcttg atcactctgt cttcactgtc gtaggcggcc agataataat
    15121 tttcctcccg ccagatcagg gcccagggac tgacgcattt gttgccgttt ggtctgggca
    15181 ccagctgctt tttaagattc cagtccaggt agacaaaaga gatctgctta ttttcctgaa
    15241 tagcccggtg gatcgcatca atgctgtaat aaatgctctc attctccgtc ttgactcttc
    15301 cggccacata gacctgtcgc tgcagtttac cggcatcgtt tttcccggcg atctgctcca
    15361 gcttccggat cagttctctg gattttttcg tggtgataaa ccgggaagac tgtaccgcgt
    15421 ccaccaacag ctttaattcc ggaagttcaa actcccggct gcccagataa tatccgcctc
    15481 ccagtctgct ctgcacctgc accacatcat agccgaaatc cgcaagacat tccatatcgt
    15541 tgtagatact cttgcgctct gcatggattc cctgattttc cagatatttg atgatatccg
    15601 gtgtgctcat tggatggttt tcatccgtgt tctcccgcag catcttgatc agatataaaa
    15661 gttttaattt ctgtcccgaa gattttgcca tggattattc ttctgtctgt ttcttttcct
    15721 ctgctgcatt ctttttcttg cgcagagtga ttcccaacgt aatggccact gcacctacaa
    15781 ccgcaatgac taccagtact accagataag ataaaaatga attcaaaaag cttactgcca
    15841 taatgtcttc tccttttccc gatatctttg tatcccgaaa ctattgcatg tattagaagt
    15901 ataccaacga aatccccgta tcgtcaagtg gtcccttcgg atatggccgc cggggtattt
    15961 atccattttc ctgtcttaag aaaattttcc gcctgtccgg aagccttctt tatgatctgt
    16021 tcctcatacc ccatcatctg aagcagacgg atcgcatttc tggtggatgc ttttcccggc
    16081 agcagctgat aggggaacag gatatcaccg tcccggatca cttcctcaaa atgatagttg
    16141 tcatagtatt ccttcaaaag ctccgtcatt tcaatatcat gggtagcggc aaaaccaagc
    16201 gttccgctct ccgccagacg gatcaggatc tgcgttgccg ctgcgatccg ctccaccgta
    16261 ttggttcccc gaagcacctc atccacaaaa caaagcaccg gagttctccg ttccccggcc
    16321 gcatccagaa ttcgtttcaa tgaccggatc tccacaatat aatagctctc cccgttttcc
    16381 atgctgtccc gcagggccat ggaggaaaag atctgaaata tcggtgcatg ataactttcc
    16441 gccgcgcaag tatggatcgt ctgtgccaga acggcattca ccgccaccat ttttaagaac
    16501 gtggatttac cggaggcatt ggatcccgta agcagaattc ctctgtccgc ccggatactg
    16561 tttttcaccg gctgtgacaa cagcggatgc cagcccttct tcatatgcag aaaagctttc
    16621 tccgtatctt cttccagttt cggaatacag tacccttcca gagatttccg aaatccgcct
    16681 acggatatgg ccatatccag gtacccggtg attgcaagca tggtttccac ttgtccggtc
    16741 tttttccgca gcttttccag catccgatta aactgaaaga tatccagatg caatatcatc
    16801 cgcagataat ccgcaaggat atccagagga tttccaccga tctttccctg atttccggcc
    16861 atgacccaat aggatccacg tcttaattcc cgcagacttt tcagtacctc ccgaagttca
    16921 tccagttctc tcctgtatac cggtattttc tgcttctcca gttcttcaca tacatcgatc
    16981 agccgtaata cataagcgaa gctgataata tacggttcga tcactttttt ctcccgaaaa
    17041 tacgtcatga tatgccagag catacacaag acaattccta tggtacccat cgcaggctgt
    17101 acgaacaata acaggagaaa tggaaggtac agcagatttc ccagcacaat cttacggttg
    17161 gaacgctccc ccagataatc caggttatcc agataatcgt agagagaata ttttcccaga
    17221 tgtcccagcc gtttcatcaa cagctggatc cttactttta tatcctcctg ctcctgcagc
    17281 cagttaacca cttcttccag atgttccatt gcctctctcc cgcaggaaat attccggagc
    17341 gtatagtaca gatattcctc tcccgccgca gaataggtcc ggtcgatccg gcgaaagacc
    17401 tcgtccattc ccagatcgtt ccatgtgata tcatccagct gcttctcttc cttatgccgt
    17461 tccagatagc tgcccagtct tgcaaaccgc tccagtgaat attccttcgg tggttctttt
    17521 ccgtaatttt cacgaagata cttcttgaaa agtttttcct gatttttcgt ctgtatcagc
    17581 tcctgcacta tcataaatat aaaaagcaaa agcaatacgc ccgcaaatac aacaaattcc
    17641 atattttttc tcacttttcc ggatataaga aggcaggcac cgccctgtgc agtgcccgcc
    17701 cccatattct gatctgtttt ttatatttct tatttgtaaa actctttatg cgacaataaa
    17761 gaactttaac aggaagatca aggataatac ataggtaagt acggatacgt ccttagcttt
    17821 accggtaaat accttgataa cggtataggt gatcagaccg ataccgatgg catgtccgat
    17881 agatccggag atcggcatgg cgatcatcat cagagccacg ggaagcgcct ggcagatgtc
    17941 atcaaaatct acattcttaa gacctgctac cataaggaca cccacataga tcaatgcgga
    18001 agaagtagct gccgcaggaa tgataccggc gatgggtgcg atgaagatac atgcaaggaa
    18061 cataataccg gtggtcagtg cggtcagacc ggtgcgtccg cctgcttcca caccggaggc
    18121 agactctacg aaggtggtaa cggtggaagt accggttacg gaacctgcca gagttcctac
    18181 tgcatcagac aaaagtgcct gcttcatgtt aggcattttt ccatccttat ccagcattcc
    18241 cgcacggctt gcggttccta ccagagtacc gatagtatca aacatatcaa tgatacagaa
    18301 tgtaacgatc agagtaattg cagtaaacca gccaatctgt gcaaatccct ggaagttaaa
    18361 cttaaacagt gtagtggacg ccatatctcc gaaggccggt acgaaggatg cagttgccag
    18421 agaagcaaag ggattggttc ccaggaaaat ggcctctcct gcccagtaga tgatgctggc
    18481 gatcagcatc cccagtaaaa cagcaccctt gacacctctc tgtgccagaa caacaatggc
    18541 aaacagacct acgaacatgg taacaaccga cagcaccatt gcggaatatc cggatcccat
    18601 attggtctta gccagtgaag gagtcagtgc accgaagaag tctctcatag catagaaagg
    18661 tccgccgttc tcggaataga ttcctgcatt ggaaccgacg ccgatgttta acagcatcag
    18721 accgatggcc ggtgcgatac cgagacgtac gcccaaagga atggcatcca caatcttctc
    18781 acggatattc agcacggaga ggatcaggaa tacaatacct tccaccagaa caatacacag
    18841 ggcagcctgg aaggatgcca cataggtcat gccggtcagg gctacgatat tggttactac
    18901 gactgcaaaa aagctgttca gtcccatgcc aggtgccatg gcaaaaggct tgtttgccag
    18961 aaatgccatt acgatggtac cgatggcgga tgcaatgcag gttgccagga aaacaccgtt
    19021 ccacagctcc ggcatggtat ccttgccaaa accggtcagc aggttcggat tcagggctat
    19081 gatataagcc atggtcatga acgtggtcag tccggcaatg atctctgtcc ttgcggtggt
    19141 gttgttttcc ttaagtttga agattttttc taacatcttt tccctcctaa gtattctggg
    19201 agccacgcgg gggagctccg ttctatgtct tctgagttat ttttcttact cagtcaacag
    19261 tctctttacg atcttctcgc attttgtgta aatacgttcc ggattctctc ccacattaaa
    19321 ttctccgtta cgatacaaaa tcttgccgcc gatcatggtc atgcagacat tggacttgct
    19381 accgctgtaa accagattct tggggatgtt gtgaatgggc tgcatattgg gctggtgcag
    19441 atcgatcata ataagatctg catattttcc ctttgcaaga acatctgcgc ggtttaggcg
    19501 cattgccttg gcaccgttca ccgttgccat cttcagtacc tccacggcat ccatgctgga
    19561 cgcatccttc tctaacagtt tgcttaagcc ggtgactaag aacatctccc ggaacatgtc
    19621 cagacagtta ttgctggccg gtccatcggt tccaattgct accgggatct tgtgccgcac
    19681 ataatcagca atgggtgcaa tgccactggc cagtttcgtg ttggagccgg gattcgtgat
    19741 cacatacata cgatgtctgc ggaagacatc catatcctgt tccgtcacat gaacgcagtg
    19801 gaaacctcct ccgccaaaat caaacattcc cagagagtct aagaacatgg gcggtgtcat
    19861 tccgtagcga gccttacact cttccacttc cttctccgtc tcggagatat gggcatatac
    19921 cggtgtccgg taatgatgtg ccatttccga cactttccag agcagttcct tggaacaggt
    19981 atattccgca tgaaatccca gctggtaact gatcagggaa ttcttcttat tccattttaa
    20041 aaactcctct tctacctgtt ttggagaaga actgaaatta ttcagtccgg ataccagtac
    20101 acaacgcatt ccggtgtcca gacaggcctc agcaatagta tccggtgtca ggtacatatc
    20161 gaaaatggaa gtcactcctg aggtcaagta ttccagcact gccagtctgg tcagttcata
    20221 gatgtcctca ccggtcagtt ttgcttccag aggaaatacc tgcttgttca gccactgatc
    20281 cagaggcagg tcatctgcca tggagcgcag gaaagtcata ccggaatggg tatgtgcatt
    20341 tttaaatccg ggcatcagaa ggtttccctt gcaatccagt tcgatatccc acgcaattct
    20401 ggggaactgg gatttgtccc attcctccgc aagttctttc tgttccgcca cataggcgat
    20461 cttgtcgttt tttacccata cttcaccata gaagacttct ctgccgggtt ccatggtgag
    20521 aattcgtgca ttatataatc tgatattcat ctgattgcat ctcctccaaa tccaaaggga
    20581 agtaattctt ttaaggtata gcgttgataa tcctgttcac tatttaccac gtagatcaca
    20641 aaatcatccc tgcaaaattc ctgcatcacc tgtctgcaga taccgcatgg atacgcatat
    20701 tccaccggtg cctctccgga tattcctccg gcaatggcaa tggctgtaaa ttcccgttct
    20761 ccctcgctga ccgccttgaa aaaggcggta cgctccgcac agttcgtagc tccgtaagaa
    20821 gcgttttcaa tattaccgcc aaggtatctg tgtccatccg cagtcagtag tgccgctccc
    20881 accgtataac cggaataagg agcatatgcc agttttctgg cttccaatgc ctggctgacc
    20941 aatgcctttt cttctgtctc ctggatcatc cgcatctcct ttttctctgt gtctatacaa
    21001 atttcttcac agattccgtt accagcttct tgaatctggg agctgccata tccgcagctt
    21061 cctgtacttc ctcatgattc agaggctgat ctgtcattcc cgccgccaga ttactgatac
    21121 aggagattcc acagatccgc attcccatgt ggttggcagc aattgcttcc accactgtgc
    21181 tcatgcctac tgcgtcacag ccaagtgtcc ggagcatacg gatctctgcc ggagattcga
    21241 aggatgggcc ggtcagctgg gtataaatac cctcctgtaa atagataccg ttctcctttg
    21301 cggtctcacg gatgatctgc tgtaagtcct tgtcatacac atggctcata tccgggaaac
    21361 gcagtcccag ttcatccatg ttggcaccga tcagaggatt gggggcaaac acggaaatat
    21421 gatctctgat cagcatcagg tcaccggcat ggaaagaagt attcacaccg cctgccgcat
    21481 tggtcagaaa cagaatctca gcgcctaaca gtttcatcag tctggtgggc agtaccacat
    21541 ccgtcacatc ataaccttcg tagtaatgta ctcttccctt catacacacc acaggaatct
    21601 catcgagata accgaagata aatttaccgg catgccccgg tacggtggaa acaggaaatc
    21661 cctcgatctc cgcataaggg atctctgtca ccacacggat ctgttccgca taatcgccca
    21721 ggcccgatcc caaaacaatc gccaccttag gtacaaaatc cgtctttttt ctcacacact
    21781 cataacagtt ctgtaatttt gcatatacct gattcatcta tatcctccgt ttagccgtat
    21841 tttcatgcaa tttagcaaat attcttcctg tttttcagaa aaataaagca aagtcaacaa
    21901 aggtattata caaaatgcac gggaaaaaag caatgggaag ctaacatccc atacaatttc
    21961 tccgtgcttc gcactctcga aattgtaacc cacattcgca tttcggacta tcgtcctccc
    22021 ctgctcatgt gtgtttactc ccctcataga aagtctgttt tgcctgcagg cagtcttgca
    22081 gactttccat gggtgggtta tggaatcata taataataca aaccatgcca ccgttgctgt
    22141 cgttgacgat cttttgcatg gtatcctgga gtttcagctg gctctcttct ccgatggaag
    22201 caactttcgt ggtgatgccg tcatccacca gctgttccac agtcttgccg aagatattag
    22261 tctcccagat accgtgctcc gcctccgcct gatcgataaa atggatcaga tcctgagcct
    22321 gctgttctgt gcccacgatg ggagcgatct ctgtctcgat atttgcgcgg atcatatgta
    22381 tggaggggct gctggccttg attttgacac caaacttgtt gccatggcgg atcaattccg
    22441 gtttctccag tgcaatgtct tctctgtccg gagtcacaac gccgtagcct ttgatcttca
    22501 ccatggacaa tgcatcctgc accttcgtat attccttttt catttctgcc aactctttta
    22561 gtttattcaa cagttggtat tcatcctcta cgttctctcc taacattccg gaaagcattt
    22621 cgtaataata cctgtcgtcg attcctacct gtaccggaac aataccgttc cacaactgca
    22681 ccggctccat cttgcagctt tgaatataat ctccctgcaa ttccacaggg tgtcccgcca
    22741 catcccgaat ccggtcatag ctgttcatca attcccggat ctgtcttaac aggtcctgtt
    22801 ttaccgcatg atccaccgga agcatatcca tccatttcgg gaaataaaat tccactgccg
    22861 tcagaggaaa ttcatacagg attttttcca gaaggcgatt aatatcttcc tttttcagtt
    22921 gctcgcagtt caccggcatc acggccacat cgtatttctt cccgatttcc tccgctacct
    22981 gtctggtctc ctcggaataa ggtttctggc tgttcaccag cacaaggaag ggttttccgg
    23041 actttttcag cgccaggatt gtcttctctt ccgctcccag ataattgctt ctgggaagtt
    23101 cccctatact gccgtccgta gtgatcacaa gaccgatggt ggaatgatcc cgcatgactt
    23161 tatccgtgcc gatctctgcc gcctgggtaa acggaatctc ttcctccgac cagggagtct
    23221 tcaccatgcg ctccgcatct tcttccatgt gtcccgccgc accgtcgatc atatatccca
    23281 cacagtcgat cagccgtacc gatacttcaa tatcatcatt cagtcgcacc tttgccgctt
    23341 cgttgggaat aaactttggc tccgtagtgg taatggtctt acctcccgcg ctctgaggca
    23401 gttcatcctg cgcccgcatt ctggcatgtt catcctccat atagggcagc accatttcat
    23461 ccataaagcg cttgataaat gtagactttc cggtcctcac cggtcccagt acccctatgt
    23521 agatttcccc tcccgtcctt tgttgtatat cccgatacag atcgtatgta ttcactgcat
    23581 agtccataaa aatacctcct gccattggtg ttatatgtat atgcaggatg caagcaatta
    23641 tgaattaact aga
//
LOCUS       DAWEJW010000035        23594 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_874_length_23594_cov_56.739539, whole genome shotgun sequence.
ACCESSION   DAWEJW010000035 DAWEJW010000000
VERSION     DAWEJW010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 23594)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23594)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23594
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_874_length_23594_cov_56.739539"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2373..2472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11849..11948
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16260..16359
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5611 a         6360 c         4479 g         6844 t
ORIGIN      
        1 gggccgatct gggctacgga attcatgaat tcttcggtgc tgccgcttct ggggggatcc
       61 atcaggacca catcgacctt cgctccctgt ccggccatct ccacgaggaa tttcccggcg
      121 tcgttctggt agaactggat gttgcggatc tggttcgcct tggcattgtt gcgggcatcc
      181 cggacagcat cggcatttaa ctccacaccg atgaccttgc cggccttgtc actggcaatc
      241 atgccgatgg ttccggtgcc acaataggca tccaccacag tctctttgcc ggtaagtccg
      301 gcatactcca gagctttccc atagaggatc tcggtctgta ccggattcac ctgatagaag
      361 gatctgggag aaatacggaa cctctttccg cacagttcat cctcaatgta tcccttgccg
      421 tagatcacat gctccttgtc gccaagtacc atgctggtat ttcgattgtt gacattgatg
      481 accacggtag taatctccgg gtaacgctta agcaatgcct tcacgaaatt attcttggag
      541 ggcaggattg gggaagccag caccagtacc accatgatct gtccggtgac atgtccggtg
      601 cggatcagga catggcgcag cagtccgtat ccggtatcct cattgtaagg gcggatatgg
      661 aatgatttca acagtccccg gatagagaca atgatctcat ccgccttggc attctcgatc
      721 aggcagctgt ccacgggaac gatccggtgg ctcttctgtt cataaatgcc ggagatggca
      781 tcgtgatacc ggttctcgcc gaataccgca tgtaccttgt tccggtaatg ctccgggtgc
      841 tccatgggga tgacaccctc gaccttgcaa aaaggagcca gaagctcctt taattgcttt
      901 tccttattct tcaactgttc cacatagggc ttgttgatcc agctgcaccc gccgcattta
      961 tcagccaccg ggcagagacc actcttcttt ttaacagccg tcttttcccg gccctgtcct
     1021 acgggcaccc gggtttcctt gcctgccgtc actttagttt ttcccgccac attggctttt
     1081 cccgttgctt tggtctttcc cgtccctttc gcaggtccgg atgttcttct gtcatttctg
     1141 ttatcgttct tcatcattcc gcctgacttc atttagtcat ccagtcttct catttctgtg
     1201 taagccaaaa gtagcggtcc cacacccttg gcatcgtctt ccacgatggg ctcggacata
     1261 taatactcga agctgccgtc acgccgacgg ttatcctcag gtcccagtcc tgccacgagg
     1321 cagataccgc cgaggcttaa atgtccgtcc ttctccttca gataacggtc acagattccc
     1381 tgaaatgcct ttaatccata agctctgtaa gactccggta aaaatccaag tctcgcaccc
     1441 ttcagcagag agtatgccat gatcgcactt ccgctggtct ctaaataatt cggctcctga
     1501 ccacccagtg ctggaagctg gtaccacata ccgctctcgt cctggaattt taacatggaa
     1561 tcgatcagat ccacgaatac cttcttcaga ttgtcatact ctgccttgta ctcttccccc
     1621 ggctcacatt tgctcagagt gtccaacaat gccatggaat accagcccag tgctctcagc
     1681 cagaaattct gggacagacc ggtctccttg ttacaccaga atgccttttt ggaagcatca
     1741 taaccgtgat agtacaatcc ggtcttggga tctctcatat attttacaac attggcaaac
     1801 tgatgaaaaa tgtcatcata gtgcttcttg tcatcgaatt tggtctcata ctccatgtag
     1861 aagggctgtc ccatgtacat gccgtccagc catacctgat tgggatagat cttcttgtgc
     1921 cagaagttgc cttcctcggt gcggggctgg gtctccacct ggctgtagat caggtcgatg
     1981 gccttgcggt atttttcctt accattcagg gcatacaatg cgaacagagt cttgcccgca
     2041 ttgacattgt caatattata ttcttccacg gaatacttcg taatcgtacc gtcttcctgt
     2101 actctgtggt caataaatgc atctgcgaat tcgtaatact tcttctctcc ggtagcgttg
     2161 tagatctcta aaagtgccag aatcatgcaa ccgtcaatgt aattccagcc ggcagctttc
     2221 ccctctctcg ccttctctat gttccatgcg ggaacatcca gagtgctttt ctcgatcagt
     2281 tcatcaatgt atttctcaaa aataggcagt tgcatgtatt atttcctcca aatttctatt
     2341 ttacggcata tagctttatt ttacacttta ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nngctgacag ggctcgtacc cggcaatgga tcaatatttt caaattccac
     2521 gatccattgt ccgtcatccg ggaaccccgg tgtctgcctg tcacaaccat catatcctgc
     2581 cgccatgatt gctgctgcca ataacagtga tccatttccc ggcagataca acggcaggtc
     2641 ctttcgtcct gtctgccggt tattgccgct tgtgacataa cagttctttg gagattcctt
     2701 caatagtaac tctattgctt tctccggctt tcctaatctt accgccgtca tcgccatcat
     2761 ggcaaaatcc catccccaca gggtaggata tttccagcat tcctctacca actgcaatgt
     2821 gttgtccata accgtcctgt ctatccggtc actgtcgatc agaccaaaag ctcccagcat
     2881 agaggggtga tctctgttat actccgtaaa tgtcgtcgga caattttcat gtgccaggta
     2941 cacgccgtct ttttccgtta attctgccat atgctccgct acctgttccc agagttcttc
     3001 cttttccctg ccgagtcgtt ctgcccacca tccggcgatc cgcaaggtat cccggaaata
     3061 ctccacttca aaagccggat tgcacacatc cattgcctta tgacattcct gtaccggtat
     3121 taccggagca cagatactgt agactttttt caccggatcc catcccacaa aatctaccat
     3181 gaaatcagcg gtttccttca ccagttccca gttctcctct aagaatctgc gatttctttt
     3241 tctgcgatat gccatttcca gcatatagat aatgtgcggc tgctgccaca ccagcaaggg
     3301 cgcaatattg gaagggcagt ccactccttc cgtggcgatc attttcggcc atcttgctcc
     3361 tttgtagccg tttcttgccg cattttctct tgcctgctgc agatgttccc tataccatgc
     3421 caggcttctg tccagcagct cctcctgatg ccacagcgga agccatgcac aatgccacag
     3481 atacatctcc agatgcattt taccgtacca gctgttacat gtcagtcctg tctcctgcgg
     3541 aggtgtcgaa ccactggaat tgattgccat aagatactgt gacaaaataa tccgccgttc
     3601 cagttccctt gctctggggt cagcgctttt attcagctga atgataccgc cccgctccca
     3661 gaaacgccta ccccctcttt tggatgcgtt cattacttcc tgtgcagata aacattctgc
     3721 ctgctctttt tgttttccta acgctatgga gatgtccagt ttttctccat tggcgaaaat
     3781 acgaagggta tggctgcctt cctttcggat ctttcctccc tgcgcataga tacctgcata
     3841 atactcatcc ctgtccagtt gtctccacag aagcattttt tcatcactcg tctgcagtat
     3901 cgtggtacgg tgtctgtcgt tctgtgtcca atccgatgcg gtaatatccg aagctccata
     3961 ggggaaatcc agcactatac taatcttttt ctccttcagt gcctcgctct ccagagaaaa
     4021 ggccaggatg tctctccctt cattgtgaca ggcggtccgc actctgcagg cattgttccg
     4081 tatctggaat tcactgtaca gaatcccttc atacagaacc agttcctgcc gcaccccggt
     4141 gatatcttct gcagaaatgc tctcctcttc ccactgcagg cggaccctta ccagattcag
     4201 tctgtgggga ttttcccgca gccagttata gacatcttca ttgcccacct ttttatcctg
     4261 cggatatttt aacagacgtc cttctctgtt tacatattcc gtcatcacca gatcatccag
     4321 tgtataattg tacttttccc tgcttaccgg tttcgtgtgc catccccatt gtgacattgt
     4381 acacaacggg agttcctgat attcctcgta tagcgtctgc attccggtga tatccgccgt
     4441 aaaagcaagt tctccgtttc ctaccgtaag cggggcgtcc gttgctatct ccgtcagcac
     4501 cggattatgt cccgatatca gtttctgcct gtctatttta tgttctgcga ccatacaaat
     4561 gctccttatc cttttactga accgatcata acacccttta caaaatgttt ctgtacaaaa
     4621 ggatataaaa taatcagtgg aatactacca aatacaataa tagcatattt taatacttca
     4681 atgttcgctg cacgcatact ttgttctgcc gtagttgcca tttcctccaa agctgctacc
     4741 gaattcatga tattacgcag gactacctgc agtgtaaatt tattctctga agacaaataa
     4801 ataaatgcat caaaatagga attccaatga gcaatggcat aataaagtac caatactgcc
     4861 aaaatagatt ttgacaacgg taataccata ttaaccagaa cccggatatc atcgcatcca
     4921 tccagtcttg cagattccaa catttctccc ggaatgctgt tctggaaata ggttcttgca
     4981 atgatcatgt tatataccga aattgctccc ggtaagatca atgcccacat actatccagc
     5041 agtccgaggt ttttgacatt cagataagta ggaatcaacg gtgctgtgaa gatcataacg
     5101 aaaataaaca gagcgctgat cacctttctt ccaataaaat cctttacgga caatggatat
     5161 gccgccataa ttgtcaatac aatatttacg catgttccca ctatagtata tataatggaa
     5221 tttttataac cggtcagaag agacctgttg gaaaatatct cctcatatgc atcaatattc
     5281 catcccttcg gccagaaaga tacctgtcct cttattacag cgcttggcga agacaaagac
     5341 gaagctacca cattgagaat tggcatcgct accaacagaa gacatatcat caggagcaca
     5401 gtatttacta tataaaatat acgattactt ttgctcataa cttttctttt catcttatgt
     5461 tcctccctat atcagactgg tatcggcaac cttatccgct attttgttta caaccagcaa
     5521 taatatcaga gataccactg actggaatat accaatcgct gtcgaatagc tatactgcat
     5581 tccgctcaga ccgatacgat aaacatacgt tgagataatg tctgaagttg ccatgtttaa
     5641 atcattttgc aagagcagta ctttttcata tcctattgtc aaaatatgcc cacaggacat
     5701 gatcaacaga attactgctg tcggcatgat actgggaatg tcaatatatc tgattctttg
     5761 ccagatattc gcaccgtcca cggttgctgc ctcatgtaat tcctggtcta ccgcagcaag
     5821 cgcagagata taaattacgg cattgtaacc cgttttctgc catacatcag agaaaacata
     5881 taatgttttg aacaatttag gctctcccat aaaatgaatg gcctgtcctc ccagattctc
     5941 gatcaggtga ttcaccggac ccgtcagaga taacatctgt gacagaatac ctacgatcaa
     6001 aacggtagaa ataaaataag gcatatacgt taccatctgt accgtctttc cgaaccatcg
     6061 cactctgaca tagttcaatg caatcgcaag aatgatcggt ggaaaaactc ccaatacaat
     6121 accatacaca gacaagccca atgtattacc catcagtctc cagaactgtg gtgactggaa
     6181 aaacatcttg aaatacttaa gtcctacaaa cgggctgtgc cagatacctc ctttgaaatt
     6241 gtaatcccgg aatgcgatct gcgatccaat aataggcata tatttaaaaa cgatcagata
     6301 aaccagaggc agtgctacga tcagatataa ctgccaatgc ctttttatgc tgcgtattgt
     6361 attttttgca tgctgttttt tatgttccgg acgaattgtt ttcttaggat tcattttctt
     6421 ccttctttct ataaaaggag ggctgcctta atgcagtccc ccttgcaaat gtttcaaaag
     6481 gctttcccga tctcctattt tgcaaaagaa gattcatcat atgccttctg taataactcc
     6541 aaatatctct ccagtcctac attattcagg gcactgatat agccatccca ttctgcatca
     6601 atatcctttg ctccggtaat aaactgcacc atatattctt tctgaacatc cataacattg
     6661 gtcttaagca aagacatttc gctggagtct tcctggctca tatataaatc cggcattacc
     6721 gactctctgg actgtcgcac cttttcatgt tcaagagacg ctctgtaaat aaccacattt
     6781 cgaccattga ccggcttaac atcctccgca tacggatccg ggttcgtagc atctgcaagc
     6841 aggaaatccg gtgtacgcac caatgccgta acctgattca aagttacatt ggctgtggaa
     6901 gcatccagtt tcttatagag tgcttttgtc acaccatcca tgcctacttc tgtcgtatca
     6961 tcatccagca tgacataatt cacaccttct tttccaagaa tagctctgta tgtctgcgtc
     7021 tcaatataat ccagcagacg gaatgctgct tcgggattac tgcagctgct cgaaataaat
     7081 gtcatacctg tctggaacat aacataagga ttccaggatg ccacacattg accgtccggt
     7141 cctgtcaaag gtgcaagtga ctggtactgc ttccatttat cggaataggg ttctgttgtc
     7201 aaatcacatg catatcccgg tctctgggcc agaaatgctc cgatgacaca ttcatcccct
     7261 gcttcattta tgtttacctg attattctta tcctgcgtaa aagattctgg gtttaataat
     7321 ccttcactat acagctgttt taaatatttc aggccttctt tgtagccttc ctgaaccggc
     7381 gcaaacgtca cttttccgtt atctaaatac aatttatttg tctcagaagt cagttgaaac
     7441 ggattcatta gaaatccatc aatctgtgta cccgctccgg aagtcactgt tgacaaagga
     7501 atttcgtcat tgagatctcc attgccattg gcatcctgct ccttaaaagc tttcatgacc
     7561 tgataaaact cttccgtagt tgtcggcatc tctaatccca gattatccag ccactgcgta
     7621 ttgatccaca atttcatatt gtattgtaca tgcagggatc catctacatt cggtaaagaa
     7681 taaatattac cgttgggagt tgttatatag tctctcatcc cctctatttc atccagtgcc
     7741 tgcttataac ctactgaaac agtgtccaga taatcgttta aaggtaagat cagtccctgc
     7801 tctcccagcg cctgttctgt tgctttgtca tagcggttac tactgcctac accagttaaa
     7861 ataatatccg gaagatctcc cgaagcgaac atggtgctca gcttttcctg tacattatct
     7921 gaagccgcac aggtctgcca ctccaaatgg attcccgttg tctcttcaag ttcttttacc
     7981 atttcattat cttcccagct gccgtccgga tttgcaggtg caaacaccgt cagagtcacg
     8041 ggctcattta caatgggaag ttgtcccggt tcattaaaca atgaattgtc ccctgtttgt
     8101 gtctgacctg cttctgctcc tgatgatcct gctgcctgtt gggatgatcc gcacccggta
     8161 aacattcctg tcataagcat gcatgctgtt aaaagtgcta caattttttt cttcttcatt
     8221 ttgtgccctc gccttctttt tttgctcagc ccacagctta ccgatttgat tttttgtatt
     8281 tgtactgtgg ctgccttacg catttcacaa tacaggccgg gcactgaaac aacaacttga
     8341 aattcctgat gcaacgtaca aaatctaaga aaatgcgccg tacactttct taggtactgc
     8401 gcattttctt agatttttat tctttccatg ccgaaggtgc tatcccgtag gcacgtttaa
     8461 aggcccgtcg gaaggttgcg ctgcttccgt aacccaccat ctgagcaatg gtctccatat
     8521 cccattttcc ttcggcaatt aatttttttg ccttttccag tcttaatttt tccaaatagc
     8581 tactaaaaga ttttcccatc gtctccttga aaaagataga aaaatagttt tctgatagtc
     8641 caaactcttc tgccaccatg gggacgccca gagatggatt gccataattt ttctccagat
     8701 attcttccag tttctgatag tatatctcgc tctttctgct cctgctgtga taattttctt
     8761 cgcatagacg caggaactgt gtttccaata cttccaatgc tttgggaaac gactgttccg
     8821 tctgtacttt ttccagcagc tgctccgttt ccatactttc tgccatatta ttacacattc
     8881 ttatcaaagt agaggtgatt cccgatataa cgactttccc tccattggcc gacagatgta
     8941 tttttcccag attttcttct cttattttta taaaaatttc ttttacctgc tctgaattac
     9001 cattttttgt agcagcctgt aatttttctt ccatttccag cggataccaa ggcatattcc
     9061 ttttccacag ctctctgctg tattccacta cattctgttt ttctattttt cccttatcag
     9121 agcaatataa cgcctgttga taagaaaata caatttctcg cttttctgta taacagctgc
     9181 cgattccgac ataaacctcc tccgctccaa gctgtttcag tttctctgct gtctgctctc
     9241 ctacaagcag aggttccata ggattttcct ctgtacaata ttgcagcaat gctacatggg
     9301 tcggatctac cggataccat atctgttctg tttccgtctc cgcacatttt tttgccgctg
     9361 acaacagttt ttctccctcc tcactgccaa acaccagtac atagtaatcc ttctgcacta
     9421 accggattcc ggcatgggca gcttcctcta taatattaag ttctgtgtca aattctccgt
     9481 tcattagtaa tgtcagaaaa tttcttctct ctttcacggt cttttcctgt agcatcgcat
     9541 tcaaagactg ataatcatcc aacagggcat gaactccctt ctccagatat tcatactctg
     9601 aaatattttc cgttttatga atttctctgt cttccagcat aaaaaatccc tgcatattgt
     9661 caatcaggtt atgaatcggt ttgctgtacc ttaatgcaaa acaacaccca aggccaattt
     9721 ccagtactgc ccctgtgaaa agaatcaacc atgctgctct acgaattctt tgaaaatcct
     9781 gcattactac cgccatcgga agaacggccg caaaatgcat tccattttct tccgatgtct
     9841 gatttaccac cagacaatct gttccattcc attttatttg ccggatatct tcccctgttg
     9901 cttcacttag aaacagatcc gtgttctccg gatcatcacc atccgcattg attttcccca
     9961 gacttgcaag cagtctgtcc tctgcatcat aaatcagaat tcctccttgt cggctcaaca
    10021 gttgtccaaa catttgctct agattttctt ttgaaagcaa aaattgtact acagcatttg
    10081 cttccggctc ctgttgaata taattccgca cagggtagga aaatgtcatt gcctgaaatg
    10141 ttctctcccc tgtatctatt tcttccaacg gaaacagctg ccacgtattc gactcgaaac
    10201 tctgttttct ccaggtctca aaatccgtat tttgataagt gcaataattg tcataaaaaa
    10261 acttatcccc aactaccatc ccgctttttc gaaatatcaa ttcatttttc ataatcacac
    10321 tgtaattccg atagtgctct gaatccgcaa aggtctggcg aagcatgtca ttgcatctga
    10381 ttaatgctgc gatattattg tccccatcct tcaatccgtc cagaaaatag acccacttta
    10441 aatcactgtc gtttgataca gaaaaagctc ctgcacacat aatatccagt tgcttttcaa
    10501 atgccatgcg gccttccgtt aagtcatttt tcataatttc actgtaatag ttctctgtac
    10561 tatttttcca aaatgaaagt agcaaaaata ctgctataaa cggtgcagcc aacagcgcac
    10621 cataagaaag cagcaatgta gagtatattc tcttcacatg aaaacgtttt aatttctgtc
    10681 gtgtcattcg tatggttatg ctcctcattt ttctcatggt ccaaaaaata tccggacttt
    10741 cgaacataaa atgtacttat ctgcaaaaaa acagcaaaca cgtacattat attacgcatt
    10801 tgctgcattg tcatcatgaa tatataattt tctcgctctg gaatgccctc gtcagcccga
    10861 ataccaacac tcccgccaca cacagattca cgatcacggt caaaagaatt tctgccggct
    10921 gataactaaa ggaaaaaata ccgttcatac actgcacact gttgtatacc gggatcagat
    10981 atcggtatag cgggatctcc tttccctgac tgagcatggg gctcaatccg ataaatgtca
    11041 ccacaatggt aaacggagac actgttgtag ctgcctcttt cacacttttg gcaaaagcgg
    11101 agaccagtgc gatcatggat accagcacca ttaccgttga cagaatgatc agaagcagca
    11161 tcgcataatc cataggtacg tacaccgagg aatccacgcc ggtcagttct ccgcccatca
    11221 ttttcggcag tgccgcaaaa gttccgataa aactggaaca gcctgccaga agcgctatga
    11281 tacttaagga aaacactttt cccagagcca gggaacttct cttcatgggt gtcaccagta
    11341 atgtcgctat ggtaccccgc tccttttccc cagcaatgga ctccggtgcc acagacatac
    11401 atccactgta caaaaacatc ataagcaaca acggcatcat catggaaaac atctgccccg
    11461 tagtatcttt gctggtggca cagtcatagt ataccgaatc tccggcattg atatccagtt
    11521 tattagctaa ggaagtctca tactgctcca gcacctccga tacctcattg taaaaatgag
    11581 cggattccgg ttccgcggaa ttgtaataaa tttctacatt gggcgcagat tccccactac
    11641 tgacctggta gttttccacc actgtatcaa aatccgccgg aaataccacc agaccatcca
    11701 cctgtttctc ctggatctcc tgccgtatct ccgtcagctg ctctctgtcc gcctgctgcc
    11761 agtccacccg cagttctcgc atctcttctc ttacggaatc cggcatgtcc accacataag
    11821 ccttcgccac ataagtatct tccggaagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnntt ggtgggctca tcgaaaatta tgatctccgg atcctgcacc agtgagatca
    12001 ccagggatac cttctgcttc attcccgtgg acaaatccct gacgcgcacc tgtgcaaact
    12061 cctgaatgcc gaaccgtgca aacagcatgc gttttctggc gtcccgtctg tctgtcggaa
    12121 taccgtacaa ttcagagaaa aagtcataca gataatccgg ggtaaaaaaa tcctccagct
    12181 tcagttcact ggtaaggaag ccgatcttgc gccgtacctc ctccggctgt gtcactacgc
    12241 tgctgccgga taacaccgca tccccgctgt ccggccggat cagcgttgcc agcatccgaa
    12301 gcgttgtggt cttaccggca ccgttaggtc ccaaaagtcc gaacacttcc ccccttttta
    12361 cgcaaaagct cacttgatcc accgctgttt tggtcttcga tgcactgtgc tccatttttc
    12421 gttgttttgc agacagatga aatgtcttgc tgatctcctg tacacggatc aactcttcgc
    12481 tgtcccctgt ctcccatttt ttctcttcca tatacgccat atcctccatg tcactgtctt
    12541 ccccgtcttc cactttacta tttacagagc atcctgcggt tcgtcagatt gcaggaattc
    12601 ccgcaattcc cattgccaca cataccagcc acaagagcag cagatgcgca ggatagaata
    12661 agtaaaagaa atatttattc tgccgccctc tcgttccgtt gtagaaccag atcagaataa
    12721 aggaaagtgc tgcaaacatc tccaggctgt ccatcaataa aaaaatgatg acaccggccc
    12781 acatctgtgc tttctttcgg ttacggaaca gataaagtac agtgattgcc aaaatcccct
    12841 taaaatcata atccgtctta agaaccaagc ccagtacaca tcctgtcaga ataataacgg
    12901 cattcagtcc catgcgcaga atcttttttc caaccgttgt acccacaaaa tgctgttcca
    12961 ccagatgaag tcccgcaatc accagcagcc ccaataacaa tgtaaaaaat acattttgat
    13021 atgccggata ccatgccttg ccgctgaatg ccagatcaaa tggcacctcc gaaatcagtg
    13081 caaataaaaa catcctgccc agatactttt tcacattgtg ggtcttttga aatccctcta
    13141 ccagtaaaaa acagtaaata gggaacgcaa atctgccgat gatacgcatt gccatgtaga
    13201 tgccgtacat tcggccgttc tgccccatcc aggcagtata ggcctcctga ttcgccatag
    13261 cttcatacag cccgttccgg atcaacagcc ttgtcacgat cacagccccg atgtgatcga
    13321 tcaacatagt cactaccgcg atatttttca gcactgcact gctgattcca cgtttttcct
    13381 gtattttccc ttccgatatt acttctgtct gcatccttat attccatcct cggtttttgt
    13441 attatttaat tagtacaata atgtatttgt tctgctttgt caagcgaccc atttgtcaga
    13501 ctcctacaaa aatgtcccct gctgacgcag aggacacact tattaacaat cttttttcat
    13561 cctaccttat atccggttcc ccataccacc ttcaggtatc tgggctcctt gggattgctc
    13621 tcgatcttct cacggatgtg gcggatatgc accgcaatag tattatccgc tccgatagcc
    13681 tgcatatgcc agatagattc atagatttgg ctgatggaca gtaccttgcc gcgctcctgc
    13741 actaggagcc ttaaaatctt atactcgatg ggagtcatct tcacttcccg tccctctacc
    13801 gtcactgtgc ggttcctgtc gttgatttcc agtccatcca cgcggtaaat actatcaata
    13861 ctttcgcaca gattgaccaa ctgtgtatat ctgcgaagct gggatttcac tctggctaac
    13921 agctccagcg gattatatcc cactgtcaca taatcatccg caccggcatt cagtgccatg
    13981 atcttggcgg tctccgcatc ctgtgcggaa atgataataa tggggatcgt agtcatatta
    14041 cggatgtcgt ggatcatctc aatgccttct ccccagcctc tgccatccag ttccacatcc
    14101 agcagcagaa gctggatctc ctgtgcacgc agtaattctt gtaactgtgc tctccccgtc
    14161 actgccacca tttttaagcc ttctcctgcg agaagggaac tcattgtctc tatcacaccc
    14221 gtattattat tgtatactgc aacccttttt tccatgctcc acaccttaca cctttcgtat
    14281 cggaaaagac ccctccgcct tatccacgtt tatcgtatcc tgtcaatgca tattttctgc
    14341 aacataatta ttaaaattct ataaacattt cgttttgctc ttatccattc ataaaagcgt
    14401 ctgcttcgca gccgacgccg ctaccgcagc tcatctttta tttcatgaat aagacgcttc
    14461 gctcatggaa tcgtaaatac actgctttgc gcgcaagcac tcacgcagct tatttacgat
    14521 ttcatggcaa aacgaaaccc tacaactaca tagacgcaat tgtagggcaa aaagttgcag
    14581 tatttttatt tttcaggtgt aaaggtttca ggtgtaaaaa acttcttcca gtaccagtcc
    14641 tttggcaggt actaacgctc ccgccttcgc tctgtctccg gactccagta catccgtcac
    14701 actctccggt gtccgtattc cctttccgac ctcgaggagg gttcccatga gaatacggat
    14761 catatgatgc aaaaatccat ttccggtaaa tacaaactcc acacggttct ctatccgctc
    14821 gatccggatc tcatcgatcc ggcggacggt ggatttctta cttttcttcg cagaggtaaa
    14881 tgccttgaaa tcgtgttctc caagcaatag ctccgccgcc tttcgcatag catccaaatc
    14941 taatgcttcc ggttcctctg tcgcatagcg tctccagaac acatccggga tctgactgtt
    15001 gatgatgcgg taacggtaac gcttcccggt ggcatttagc ctgctgtgaa atctcggtgc
    15061 cgcttccgac acctccacca ccgcaatgtc ctctggcaga taccggttac aataggccat
    15121 gatctcttct gtactcatgt cagattccgt atggaaattc gccacctgtc ccaacgcatg
    15181 cactcctgca tccgttctgc cgctggctgc gatctccaca ggctccccgc acattttcgt
    15241 caaaagaact tccatttttc cctgaatcgt attgtctgtg gatgtctgtt tctgccatcc
    15301 ctgatatctg gttccttcat attgcagtcg tagtctgata tttctcatga tttcttttct
    15361 ttctgttttt tcgcagaagc ttctttttga gcttcttttc gagcttcctt ttgggcttct
    15421 ttttgagcct cttcctctgc tgcctgtctg gccagttcct cgctcagcct ccggtctctc
    15481 tcctgcatct gccgcacttc atccatatcc tccgacttca gtctacggaa atgattgatc
    15541 ccggctgtca gaatcttcac gctgtcctta gtcgtctgta aagcgccctc ctgatacatg
    15601 gtcagagcga tcagaccaat gggcactgcc acgatcattc ctacgacacc gcccagctta
    15661 tatcctatgt acaggaggaa cagggtcggc agaggcggca ttcccacgga atctcccacg
    15721 atcttcggct ggatcagctg acgcgccagc tgtccgatgc cccagatgat cagcagtcct
    15781 atggccattt tataatcaga acttaagatt ttgataattg cccaggggac cataacagtt
    15841 ccggttccaa aaaaaggcag gaaatccaga aatgcgattc ccagtgcgat caacaacgca
    15901 taatctacct gcagaatagc aaatccgatg actaataaca gatacatcca cacttcgatc
    15961 ttaagctgtg ccttaagata tccgcctact gctttgacca gtccgcgctt cagcatataa
    16021 taccaggaca gtacttccgc cggtgtatga ttacggaacc agactgccag ctggtctctc
    16081 tcagccacaa agaaatagga agaaagcagt gccatgatca gtccgatcag gatcgaagga
    16141 acctgcttgg caaaattgcc cacggcattg atcgtagggg tgccgatctt acccagcaga
    16201 tctcccacat agcttccgat ctgattgatg acatccatga tgctttgctg tacatccgcn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aaaatactaa cagcttcggc
    16381 accaggaaaa cgatcaacag caatataata aatgccacac ccagattcac taaggcttta
    16441 caatatttct tcatatgctc tcactctctc acacattctg cttttacact cattcatcct
    16501 tcctgctgtc tattcctgtg caggatcttc tgctgccagc actccgtcca ggatctcgta
    16561 gatttcttcc cttccctgtt tcgtctgtgc ggaataaggt acgatctgtg tatccgctcc
    16621 ggcctgcagg gtggtgcgga tcagcctcac ctgcttctgt atctgactgc ggttgatctt
    16681 gtccagcttg gtggcaatga tgatgggatc gtagccattg cgacggatcc agtcatacat
    16741 gatccggtcg ttctctgagg gagcatgccg gatatcgatc agcagaaata ccagcttcag
    16801 ctgtttggat ttatgcagat aatcttcgat catcttaccc cactgggctt tcacatgctc
    16861 attggcctgt gcatagccgt agccaggcag atctacaaag tagatttcat cgttaatgtt
    16921 gtaataattg atggtctgtg tcttgcccgg ctgggaactg gtcctggcca gagacttacg
    16981 gttcatcaat ccgttgatca gagaggattt gccgacatta cttttcccgg cgaatgccac
    17041 ttcgggatgt atattctccg gaagcttgct ggtaatacca cagaccgttt ccagacttac
    17101 attctttatg atcataaaaa ctccatttct gcgctgcttt tgcgcatctt aggtttgtac
    17161 ttttttgcac aaaacgctgc cctgtgtgac cacaaggcag cgcccggatt attttgcctt
    17221 tttcagagat tcatcatgta tggtgagggg ctcgctctct ccatccactg ccgccttggt
    17281 gacaacacat tcttcgatgg tgtcatccga aggaatctca tacatggtat ccatcattac
    17341 ggattccata atggagcgta agcctctggc tccggtcttg cgttccagtg ccttgtcagc
    17401 aatagcctcg atggcatcct gctcaaagct caattctacg ttatccatct ggaacagctt
    17461 ctggtactgt tttaccagag cgttcttcgg ctcttgaaga atacggatca gtgcttctct
    17521 gtccagtcct cttaaggaga cacagatagg aacacgtcct acgaactcgg ggatcagacc
    17581 gaacttcacc agatcatgcg gagttacttc ctgcagcagt tcgctgatat ccttatcctc
    17641 tcttctgccc agctccgtat tgaaaccgat ggccttacga tccagtctgg actctacgat
    17701 cttctccagt ccatcgaaag caccgccgca gataaacaga atgttggtgg tatcgatctg
    17761 aatcagctcc tgatgaggat gctttctgcc tccctgggga ggtacgcttg ccacagtacc
    17821 ctcgatgatt tttaacagag cctgctgtac accttctccg gatacatcac gggtaatgga
    17881 tacattctcg ctctttttcg tgatcttatc gatctcgtca atatatacga taccgtactg
    17941 tgcacgctct acatcataat ctgccgcctg gatcagcttt aacaggatgt tctccacatc
    18001 ctcacctaca taacccgctt cggtcagtgt ggtggcatcc gctatggcaa acggtacatt
    18061 gatgatcttg gccagtgtct gggccaggta agtcttaccg gaaccggtag ggcctaccat
    18121 gataatatta ctcttttgca gctccacatc attcagatcc ttgggagccg ttactctttt
    18181 gtaatgattg tacaccgcaa cggagaggac ttttttggcc tcctcctgtc cgatgacgta
    18241 ttcatccagg aagttcttga tctccttggg tttcagaagg ttaatgtcgg gactcgcagt
    18301 ctcttccact tcttcatctt ccagttcttc ctccaggatg tctgcgcaga tgtctacgca
    18361 ctcatcacag atatataccc cggcaggtcc cgcgatcagc ttgcggacct gactctgggt
    18421 cttgttacaa aatgaacatc tgatgtcatt tccggaattt tttcctgcca ttattatact
    18481 cctgttattt tatctccttc cgaagacgcc gcttttgcgt cgtaggaaac gcgagccaaa
    18541 tttcagattt ggctctgcga taaaagctac tttgtgccgt atgcggcaca aatagctgtg
    18601 taaaaaatat gctatcaagc atatttttta cactatgcct cgtggaaggt gatcacctta
    18661 tcgatcagac catactcaca agcttcctca gcgcttttcc agttatctct ctcggtgtct
    18721 gcgcagatgg tctcataatc cttgccggtg ttctcagcca gcatacggtt caacttctcc
    18781 ttggtacgaa gaatatgctt tgctgcgatc tcaatctcgg tagcctgacc ctgtgttcca
    18841 cccagtggct gatgaatcat aatttctgca ttgggcagtg caaaacgctt gcccttggca
    18901 ccgcctgcta aaaggaaagc tcccatgctg gctgccatac caatacatac ggtggacaca
    18961 tcgcacttga tatatttcat ggtatcataa attgccatac ctgcagtaac gcttccgccc
    19021 gggctgttga tatacaaatg gatatcctta tccggatcct ctgcttccag gaacagcagc
    19081 tgtgctacaa tgatgctggc agaagtctca ttgacttcct ctcccagaaa aatgattctg
    19141 tccttcaaca gtctggaata aatatcataa gaccgctcac ctttgctggt ctgttcaatg
    19201 acgtaaggta ctaaactcat attctaaaac ctccattgct tctgattaat attactatcg
    19261 tatcctactt atcaggcttt ttcaacgaga ttccgaagat ttaataaaaa tttaaaagaa
    19321 agcgtatccc cacctccctg cggatgtggg aatccgcttt ctctttctat cgtatcttaa
    19381 agcatatatt atgcttcttc gggcttctct tctgctgcct tttccttctt ggtggtcttc
    19441 ttggctgcag tctttgcttc cttagcgttg tctacgatga aatcagctgc ctttcttaca
    19501 cagatatctt ccttcacctg cttagcgccc tgctcaccca gaagctcttt taccttgtca
    19561 gcttccatct ggtaagcttc tgccatgctc ttaacttcag cttcgaagtc ttcctcggta
    19621 gcttccatct tctcagcagc tgctacagct tccagaacca gacgggactg gattctcttc
    19681 agtgcttcgg gcttcagctg ctccatcatg gtctgagcgg tcagaccggt gaactgcatg
    19741 tactgctcca gagtgattcc ctgtgcctgg attctctgtg caaagttctc taacagctgt
    19801 ctctgctggg tctctaacat agcatcgggg atctccatct gagcgtctgc gatcactgca
    19861 tccagagctg cctcttcctt ggcaatctta gcttcctctt ccttcttgga agtcagcttc
    19921 ttctgtacat cttctctata ctcagccata gtatcgaaat cggatacctc gcttgcgaac
    19981 tcgtcatcca gatcaggaag ctgcttctcc ttgatctcct taacggtgca cttgaatact
    20041 gcaggcttgc ctgccagatc ggttgcatga taatcctcag ggaaggttac gtttacttcg
    20101 gtctccttgt tcagttcagc accaaccagt gcctcttcaa aaccgggaat gaaggaaccg
    20161 gaaccgatgg tcaggggata gttctcaccc ttgccgccct caaatgcaac accgtctaca
    20221 aaaccttcga aatcgataac ggtcatatcg ccgtccttaa cagcacggtc ttccacgctg
    20281 atggttctgg cattgctctc acgctctctg ttgatctcag cgtcgatatc ctcatcgctt
    20341 accttaacgt cgatcttgtc aaccttcaca cccttgtatt tgccaagagt aacttcaggc
    20401 ttcagagcaa cctctgcggt aaagatgaaa ggcttgccgg cctctaactg ggtcacatcg
    20461 atcttcggag aagatacgat ctgctcctca cactcttcca gagccttatc gtaagcatcg
    20521 ggaatcagtg cgtttgctgc atcctcatag aatacggact tgccgtacat ctgctcgatc
    20581 atcttacggg gagctttgcc cttacggaaa ccgggaatgc tgatcttgtt cttattcttc
    20641 tggtaagcac cttcaattgc cttctccagt tcttcagcag atacttcgat ggtcagcttg
    20701 gccatgtttt tctctaactt ttcaacctgt aaactcatta taacatttcc tcctttatac
    20761 ccttgcgggt ctgtatccag tcgcagtcat tttgcaatta tagcataagt tggaggaatt
    20821 gacaaccttt ttattttggg gctttttgcg catccggggc atacaggcac ttcgtgctga
    20881 ctccggggat cattcccagc ttccccgaca gggcactgac tttttcctgg ggggcatcca
    20941 tgaccacact gatgatgttc atctgctttt catgataagg tatccccatt ctgccgatga
    21001 tatattcccc atattcgtgt aaaagtccgt tgatccttat cgccgcctcc ggatcctcca
    21061 caataatgcc gatcagggtc actcttgtat tttccgtacc tttttccata tcggtcttcc
    21121 tcctgtggtt tctatatctg catctgcgtc atttccgaaa attcttctcc ggttctgata
    21181 tgagtagcta tcatcacgat ccggcctctc gtcttcctcc ggatatagtc ttcggccttt
    21241 ttgcggtttt cttcatccag tccggtaaaa ggctcatcca gcagaacaca ctgcgcttcc
    21301 gcctccatgg cccgtaccag agacactctc cgtttcatgc ctcccgatag ttcgtggcag
    21361 ggctgatcca acgcttcttc cggcaacaac tgcgtcaatg ccgttctcgc ctgtgaagca
    21421 tctcccagga tcatttccag attcttcacg gcactacagt cttcacacaa acgatcctcc
    21481 tggaattgca tggcaaaagc atccatttcc gacacttctc cctcctccgg ccgctccagt
    21541 ccgcacagac accggaaaaa agtggtcttg ccactgccgg aagggttatc cagtacatac
    21601 ggttccccgc tgtgaaattc gtgatccaca tgtcggaaga tccattgatt gtgaaaactt
    21661 tttccaagat tccttacaca cagggtgcgc tgttctctac cagcgatttt ctgtggcatg
    21721 gagggatcct tacaggcagg ctcccaccgg aaaaagactt caatcccgta gaatattatt
    21781 ttttcaaaaa atacactgag tactacgatc accgctgtcc aggcaaacag atccgccgta
    21841 tccagataaa tcttcgccag atacagtgct ccaccgatgg aatgactggg agtgccaatc
    21901 acttccgccg ccactccgga cttccagcac attcccagag atagctgaaa cgcgctgagc
    21961 aaaaaaggct ttaacgccgg acggtaaatg tagaagaact gagtaccaag gggaaggcgg
    22021 aatacctccg ccatctctaa cagccctctg tccgcgctct ttagtccttc cagcgtattc
    22081 aggtaaatat tcggaaatac taccagaaaa cagatagcca ccgccagcat ggaagaaccc
    22141 caccagatca gcaaaagcac tgcaaatacc gctaccggta ctgccttgca gaataccatg
    22201 aaaggacgca ggacttcttc cgccagcgga taacggtagc tgatggccgc cagaagaagt
    22261 gccaataccg cccccagcaa aaagccgaag gcgatccgcc acagcgaatc aaaggcactc
    22321 cgccagaact ccacctgccc ggccagagta agcagtgccc gcaatgcctg caaaggtgtc
    22381 accaccagca ggaaattgtc cacacacatt gccagaatct gccatactcc cagccagaac
    22441 aacagtatta taaatttttt aacgtatatt ttacccttat tctgcatcat aatagaaatc
    22501 ggattccgga agtccgccac ctatggacag ggaatcctgg tgaaacagta cgtcaaggta
    22561 tccggaaagt gcctgcttca tttcagcttt gtcaatatag gtaatgttgc agttcggaat
    22621 tgctttctga gcaatgggct ctttggctac gatctgggcc tccactgcca gtgcagcacc
    22681 ggtggcaggg tctgcattga tgtcttccgc acttgcctta tgatcttcca taaaggattt
    22741 cactgcttct tcgttctcct cgaggaattc cttgcggacc accgttacac cggtgaccat
    22801 gctgctgccg tcctctccct gtaccttatt ccactcttcg tttaagtcga agatcacctt
    22861 cagtgcatca ttctgcatgc aggctgcggt aacaaaaggc tggggcaaca ggccgatggc
    22921 tgtgggatct tctgccagta cagatgccac ctctgttgct tcggatttat actccagtat
    22981 cacgttatcc gcagacatac catttgctgt cagcaggtac tgcaatacat agtccggtgt
    23041 ggttcctttt ccagtcagat aaatggtctt accctttaga tcggtaaagt ttgtcagacc
    23101 gtcttctccg gataccatat aaagtacacc cagagtattg atatcaatga cttccacacc
    23161 gccctgtgtc ttatgataga gaacgcttgc cacattggcg ggtaccagtg caatgtccag
    23221 atctcccttt accatcagcg gcaggatctc atctgcacca gtagccatct ggaattcata
    23281 ggtattggca gtctcaccct tgtctgcttt attcattaaa aataaaagtc ctaatgaggt
    23341 cggtcccttt aaggatgcca cacgtaccag cgtgccatcc gcggtctccg tattttctgc
    23401 tgtggcttct gtgccttccg caacggtttc cgtactctcc accgtttcag tgggctgtac
    23461 gctctccgtt gccagatctt ccgatactgt ttccacactc tgtgcctgtc cgcatccggc
    23521 aagaccgctg cacaacagtc caagagtcag tgcaagtgcc agagtttttg tgatttttgt
    23581 tttctttttc attg
//
LOCUS       DAWEJW010000036        23358 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_888_length_23358_cov_55.258894, whole genome shotgun sequence.
ACCESSION   DAWEJW010000036 DAWEJW010000000
VERSION     DAWEJW010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 23358)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23358)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23358
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_888_length_23358_cov_55.258894"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5519 a         6499 c         4785 g         6555 t
ORIGIN      
        1 cgatagatct gttcgcacag gatcactcgc atcaactgat gaggaaatgt cattttggaa
       61 aagcttaagg ccagatccgc tcttttacag acacttggat gcaaaccaag cgatccgcca
      121 atgacaaaac agatatggct ttttcctctg acagtgcaat cttcaatcca tgccgcaaat
      181 tcttcggaag aaaagttttt accggcaatc tccagcgtaa cgatcagggc atcctgtggc
      241 agtttcgcca ggatccttgc cccttctttt tcacggatct gatcttccag agccgcactg
      301 gctccatccg gtgtcatttc atccgtcact tcgatgatat ttaacttcgt ataacgcccc
      361 agacgtttgc tgtattcttc tatggcgtcc cggaaaaatt tctccttgat cttccctaca
      421 catacgatgg atattttgat cattcttctg ctccgtttgt atccgggaaa atctgttcat
      481 agatctttgt gtcaataagg ttctccagca tctgctgatc cagattcata aactgatgat
      541 tcatctggtt gcggattact tcgaatctct tgtaataaag agcctcttcc ggtgtatctg
      601 cagcattttc aatctgagcg tcaatttttt ccgccgtcag attttcttca ctccacttca
      661 gaagagtatc caccagagag ttctcggact ctgccttttt ctcaaaaagc tttgcattcc
      721 gcatggatac cacgcccatg acaatgaaca gaatgaaaac agcactcatc actccgtaga
      781 acatataggg gttgccaatg ttcaaaggaa gtacgccggt aattcccagg atcatgacaa
      841 gcattccgag gattcccacc accagcagta cccaggcaga ggaacgattc tcctctgctt
      901 tggctccact gttcatataa gtaagtccac cctgggcgga agctgttttc agcagttctc
      961 cgggattgac tttctccgat gcttcttctg tctccggcat caggaattca tctaccgcag
     1021 cctcttcctc agcagcattt tccgtccgct ctttttcctg ctgttccaga aatacccggg
     1081 caatcgtctt cgcctgtttt tcctcttttg catctacaaa cagttcatag acctgctccg
     1141 cctcatcgaa ggaaagtttg ctcttgcgga tcccattgta tgtaaggaag tctttcagtt
     1201 tctccatttc gtcctgttca ccgaacagca cggaagtcca gtccgtcaat tcttcctcgc
     1261 ttaccagcgt ttctccgcaa tcgggacata cggtgattcc ttcccgatat tcgtttttac
     1321 atttcggaca ccatgccata taccaacacc atccttaata ctcgtaatcc aggcaggctc
     1381 tgttgcctgc cacggatcca atatattcct ttgctgccag atgatccgga tgcacctgat
     1441 aggcagccag cgcctcagta tctgtaaact cagagatcag agccacatcc cggttgctgc
     1501 tgtccagttc attgatgatc acttccacct ttacggcacc gggaaccttt tctttgatgg
     1561 gctccagtaa ctccttgatc ttcccggcgg tctctttctt ttcctgtttt gtcagattgc
     1621 cttccagact ccacagaaca atatgtctta ccatgtaatc ctccattcca tcctgtctgt
     1681 ggacaggatc cggggtgttt attttgtact cagatattcg tcaatgcctt tggcagcagc
     1741 ttttcctgcg cccatggcca gaataacggt cgctgctccg gttaccgcat ctccgccggc
     1801 atagacacct tcctttgtgg tcttaccatt gttctcgtcc gcaatgatgc acttccactt
     1861 gttggtatcc agtcccttgg ttgtagaaga gatcagagga ttcggggaag tacccagaga
     1921 catgatcacg gtatccagtt ccatcacgaa ttcagatccc ggaacctcta ccggacggcg
     1981 tctgccgctc tcatcgggct cacccagttc catgcggatg cacttcatac cggtcaccca
     2041 tcccttatca tcggaaagaa tctcagtagg attggtcagc agatcaaaga tgatgccttc
     2101 ctcttttgca tgatgtactt cttccactct ggcaggcagc tcctcttcgc ttctacggta
     2161 tacaatgtgt acctccgcac ccagacgaag tgcggttctt gctgcatcca tcgccacgtt
     2221 accgccgccg acaacggcta ccttcttacc atgcatgata ggagttctgg agtcgggatc
     2281 aaaagccttc atcaggttgc ttctggtcag gtactcgttg gcagagaaaa caccgttggc
     2341 attctcaccg gggataccca tgaacttcgg cagtccggca ccggaaccga tgaatacagc
     2401 ctgaaaacct tcctcatcca tcagctggtc gatggtcacg gacttaccta ccaccacgtt
     2461 agtctcaata tgaacaccga gagattttac attctcgatt tcttttgcca caacaccggt
     2521 cttgggaaga cggaactcgg gaataccgta taccagtaca ccgcctgcct catgcagtgc
     2581 ttcgaaaatg gtcacatcat atcccagctt tgccagatct ccggcacagg taagaccagc
     2641 agggccggaa ccgatcacag ctaccttctt accgttcttc tgtgctgcgg gagtgggctt
     2701 gatgccattc tctcttgccc agtcagccac aaaacgctcc agcttaccga tggagatcgg
     2761 atctccctta aagccacgga tacattttcc ttcacactgg ctttcctgag ggcatacacg
     2821 gccgcagaca gcgggaagag cgctggattc actgataata cgataagcct cttcaatgtt
     2881 gccgttcttc acctgttcaa tgaactgtgg aatctggatt gctacggggc agccctttac
     2941 acactgtgca tttttacagt taatgcagcg ggaagcctct tccattgctt cttctttgtt
     3001 atatccgaga caaacctctt tgaaattggt agcacgttcc ttcgcatcct gctctctgac
     3061 aggaattctc tttaatacat ccattttatt ttaaccatgc ctttctgatt tatcgtgttc
     3121 aaacttttgt atgactactg gcagttgccg cagccaccgt gatgggtatc tccctctttc
     3181 agggccagca ttgcacgtcc ttctgcagtc ttgtagatct gctgacgtct cattgcctca
     3241 tcaaagttta cttcatgtcc atcgaactcc ggtccgtcta cacaggcgaa ctttaccttg
     3301 ccgccgacag ttacacggca tgcgccgcac attcccgtac cgtctaccat aataggattc
     3361 agactgacaa ctgtgggaat ctccagttct ttggttaaga gacacacgaa tttcatcatg
     3421 atcataggac cgatggcaat acacagatcg tactttttac cttctgccac cagatccttg
     3481 atggtctggg ttaccatacc gcttcttcca taggagccgt catcggtggt tacatacaga
     3541 tttcctgcga cagcttccat ctccttctcc aggatcagaa gatctttggt cttgcttccc
     3601 acgataacat ctgcagcaac gccatgctca tgcagccatt ttacctgggg atataccggt
     3661 gccgcaccga caccaccagc cacgaagagg atcttctttg cctttacctc atccagaggc
     3721 tgttctacca gctcggaagg acatcccaga ggacctacga aatcatggaa gtattctccc
     3781 tcggaaagct gtgccatcag ttttgtaccg gcaccaacgg tctggaacac aatggttact
     3841 gttccttttt ctctgttgta atcacagata gtcagaggga tacgctcact ctcgctatcc
     3901 attcttacga tgacaaactg accgggttca caggtctttg ccactctgtt tgcttctacc
     3961 tccataagat agatgttggt tgtcagctcc tgttttttta cgatcttata catttctctt
     4021 tttccgtccc ttctgttagg attatatatc taaacggtca ccaagcgtat aatggcggcc
     4081 gctttcgtcg gtctgcaatt tgtacagttc ccgggtatag gcatttacgg cgaacagatt
     4141 ccccgtcttt gtgactgtcc catcttctcc ccggtagatc tcggcaacaa catagaaatc
     4201 atctatcttg ttgatgttcg tcacatactg atactgatag agatatgccg cagcggattc
     4261 atcaaagctt ccggatgctt ccgttatttt accactttct atggcataat tcactacttt
     4321 tgtcacggca tcctccggca taaactccgt gttcatgacc agattgtagc ttttctccac
     4381 gatctcgctc tttcttcctt ccgcaatgcg gataaaccgg aagactgatt tgccaagagg
     4441 catggggatg ggtccattat acaccgtgga attctgcgta ggatcggaac catccgttgt
     4501 gtaataaaca tcatatccgt cggtcacctc gatattgatt gggaattcat aatccccact
     4561 gaccgccgtt acctccggag cactcagttc ctcgatctca atatggtaat ttttggtaac
     4621 cactggacta ctgatcccat tctcattgat gtagacggct ttgatacagt aatcaccgct
     4681 ctctaaaagg atgggtgccg tatacagcag actgtgttct cccggttcgg tttcgtcggt
     4741 agtatagtag atcttaccac ttccgaaagt tgtcagtttc aaaggctgga tgctggtata
     4801 atatccctcc tgcagactga attccggttc cattgccata tagctctggt atttactcat
     4861 aatatttaca ttaccacagt tcaacaacag atcattaatg gtcttataat cttccctggc
     4921 cgcatagatg gcgatcagct ttccataagc actttccagt tgttcggaag tagctgcggc
     4981 atccgcaaca atctcccgaa gcagatattc atattccatt ttgttgttct tctgaaaata
     5041 aatatccgcc agttcaaatt ttaaggtgat gttccccgga tcgatttcca acgcacgttc
     5101 ataacaggtg atcgcctgat cgtattcccc ggaagctaca tactctctcg cactctttat
     5161 ctgatattca gcggaatgat agtgggaata agagattccg aaaccggtac ataccaccgc
     5221 tactaccgca agcaccagga tcagcacaat gaatttccag cgaaagggat gttttgccgt
     5281 attttccagc ggttcttcat cccagtcctg caattccgtc atgctctgct gtgcatcctg
     5341 atcgtccgtg gcatcttctt ccggtgtcat ttccctggca atatcatgca gagtctgctc
     5401 catattgtat tcaatttccg gttcaaaatc gggaacaata tggatatcct ccccacagtg
     5461 ctcgcagtaa agcgtacctt ctttcatttc cgccccgcaa ttcggacact tcatataatt
     5521 atcctcttac tgcctgcaag gataccgatt ccaggcagtt attcatcagc atagctatgg
     5581 tcataggacc tacgccgccg ggaaccggag taatggcgct gcatacaggc tccacactgt
     5641 caaagtccac atcaccgcac aacttgtttt cttcgttgcg gtgaattccc acgtcaatga
     5701 caaccgcacc ctcttttaca tattctgccg tgatcatctt cggcttgccg acagccacca
     5761 ccagaatatc cgctcttttg gttacttctt tcagatcttt ggtacggctg tgagccaccg
     5821 tcaccgtacc gttctcccga agcagcaaca atgccatggg cttgccgacg atattacttc
     5881 tgcctaccac cacacactct tttccggcga tctcaatacc ggaacgcttc agcagctgta
     5941 tgactcctgc aggagtacag gacacaaagc ccggcttgcc gatgcagagt gcaccgacat
     6001 tctggggatg aaatccgtct acatccttta aaggagaaat ggaatccagt accttttctt
     6061 cgtcaatatg tttcggtaac ggcagctgca ccagaatgcc gttgacatcg gttctgtcat
     6121 ttaattcccg gatcagctcc agtaattttt cctctgttgt ctcctctgga agttcatatg
     6181 ccagagaccg gatacctaca tattcgcagg cctttttctt gttgcggaca tatacggaag
     6241 atgcgggatc cgcacctacc tggatcaccg ccagcgttac ttcgcagccc tgctctttta
     6301 accctgcaac tttttctttg acttcatctt taatctgtgc ggaaataact tttccatcta
     6361 ttaactgtgc catgagacac tttcctcctt actagaacaa gcctacgatc ctaccgcagt
     6421 catccacatc gatattgtaa gcagctggag ccttgggcag gccgggcatg gtcatgaccg
     6481 caccggtgag cactacaaca aaaccggcac cggcagacac atagacctct cttacattca
     6541 attcaaaacc ggaaggtctt cccagtaacg taggatcatc tgacagagaa tactggttct
     6601 ttgccataca taccggcaga tcaccaaagc caagttccgt cagtctcttt aactgttttg
     6661 ctgccgccgg agcaaacact acatcagacg ccccgtagat ctcctgcgcg atctttttga
     6721 tcttatcctg aagaggcagt tcattttcat ataaaacatg gaaatggttc tctttttcat
     6781 cgattgcttt taatactttc tcagccagag cgattccgcc ctctccgcct ttctcccaca
     6841 cttcggagag tgcgaactca cagcctcttg tctcacagaa ttctctgaca taagcggtct
     6901 ccgcctcact gtcggtgata aaagagttca gggtaaccac tacaggcaca ccatatttct
     6961 gaagattctc aatatgtttc tccagattca caataccttt tttcaacgct tccagattct
     7021 ctgctcccag atccgccttt gcaacgccac cgttgtattt cagtgcacgg atggttgcta
     7081 ccagtaccac tgcatcagga gtaagtcctg ccttacgaca tttgatatcg aaaaatttct
     7141 ctgctcccag atccgcaccg aagccggctt ccgtgatgca atagtctgcc atcttcatgg
     7201 ctgccttagt agctctgacg gagttacatc cgtgagcgat gttggcaaac ggacctccgt
     7261 gtaccaatgc tggagtatgc tctaaggtct ggatcagatt gggcttgatg gcatccttta
     7321 acagtgctgc catggcaccg actgctttca gctgaccggc ggtcaccggc tcaccctgat
     7381 agttgtaagc cacaacgatt ctggacaggc gctccttcag atccttcata tcgtctgcca
     7441 gacacaggat cgccatgatc tcactggcta cggtaatgac aaaatgatcc tctctgacaa
     7501 agccgtctgt cttgttgcca agtcctacca caatattacg aaggactctg tcattcatgt
     7561 cctcacagcg cttccacacg atctgccggg tatcgatgcc cagatcattg ccctgctgga
     7621 tatgattgtc cagcaacgca gccaacaggt tgttggcaga ggtgattgca tgaaaatcac
     7681 cggtaaaatg cagattcagt tcatccatag gcactacctg tgcataaccg ccgccggcag
     7741 ctccaccttt gataccaaag caggggccta aggacggctc tctgagcgca attacagcct
     7801 ttttgcccat tctgccgaaa gcttctccca tacctaccgt ggtcgtggtc ttaccctctc
     7861 ctgcaggagt tgggttgata gctgttacca gaatcagttt tccattggga cgatcctgga
     7921 tcttacgtaa aaattcttcg gaaagctttg ccttgtattt tccgtaaagt tccaggtcat
     7981 ctgcttcaat gccaatactt tcagccactt tggtgatggg ctccattacc gcttcctgtg
     8041 cgatttcaat atctgttttc atgtcctttt tccctccgtt tattcagaca gtctcgaacg
     8101 aatgtcagat ttcctccaac agctgttcga actgttccat gctcataagt acttttctgg
     8161 gtttagtgcc ttcttcttca cctaccacgc cgtattcgca aagctgatcc atgattctgg
     8221 cagcacggtt gaaaccgatt ttcaacacac gctgcaacat gccgatgctg gccttgtctt
     8281 tatcaataat aaaccttgcg gcctcctcaa aatattcgtc tctgtcgtta ccgccaccag
     8341 aaccgcttcc ggaggaacca cctgacgagc cgatattttt gatcttctct tcaatatctt
     8401 ctgcatagtt gctataggta ttgcccagtg cctggttctt gagataatcc accacatcgg
     8461 atacttcctt gtcggacaca aaagcgccct gtactctggc cggtttggaa taaccctgcg
     8521 ggtagaataa catatcgcct ttaccaagca atttctccgc accgttcata tccaggatcg
     8581 ttcgtgaatc cacaccgctg gatacggaga atgccacacg actgggcata ttggccttga
     8641 tcagtccggt gatgacatcc acactgggtc gctgggtagc gatgatcaga tggatacctg
     8701 cggctctggc caactgtgcc agacgacaga tagattcctc cacttcgccg gggcatacca
     8761 tcatcagatc cgccagctcg tccacgataa tgacgatctg tggcatcttc ttcggtgctt
     8821 cctctccttc cggaacaggc atagtctcta ttttcttgtt ataccccttc agatcacgga
     8881 cattataatc ggcaaacttc cgataacggt cttccatctc actgacgccc cagtgcaatg
     8941 cagcagatgc tttcttggga tctgtcacta ccgggatcag caaatgagga atgccgttat
     9001 agacactcag ttccacgacc ttcggatcca ccatgatcag tttcacatca tccggatgcg
     9061 ccttgtacaa aatactcatg atcagcgtat taatgcatac ggacttaccg gaaccggtag
     9121 ctccggcgat cagcatatga ggcatcttgg caatatccgc caccacagtc ttgccagcga
     9181 tatccttacc tacggcaaat gcaatattgg aattaaactc ctgaaactct ttgctctcta
     9241 acagatcccg gagtgctact gtcatgttct ctttgttcgg gacttcgata ccgatagctg
     9301 cctttccggg gatcggtgcc tcaatacgaa tatccgttgc cgccagattc agcttgatat
     9361 catctgccag gcctacgatc ttggatacct ttacgccctg ttccggctgt agttcatagc
     9421 gggtaacact gggaccctgg ctgatatcgg taatcgttac ctttacaccg aaagtattta
     9481 acgtctgctg caagcgcatg gcggtctctt tcaattctct ggaggaatct ccggtagctg
     9541 cttttccctt ctgtaacagg ctcagaggcg gaatccggta ttctgccgga ggctgtttgg
     9601 ccgctttcgc aatatcctta tgtatctgat cctctgccag agaattaccc tttggcggct
     9661 ccggtgccgt gattttcggt cgtatgcggc tttccgccgt tgctgtctct gcaggttccc
     9721 gcacgacagg ctcagtctcc ggctgcagct gccgcatatc ggaggatgac tgccattccg
     9781 taaccggttc ctctacaggc tccgggatat cttccttgtg aatccggata gattccgcct
     9841 gaggtgtcag gagtgctgcc tcctcctgcc aggattctct gcgttcaaaa ggttttttga
     9901 aggtagcagc actggtcact tcctccacgg ggatctcctc gtaaggttct tctgctgcgg
     9961 cctcctgcat ggtcacctga tgcattcccg ttaccttgat cttatcaaaa tcgctggagg
    10021 tcactctggt ctcttccaca acaggaatct cctgtgcatc ctcttcataa acgatctcat
    10081 gaatatcttc ccgtcttccc acaggctgtg cctcctgctt tttcagggaa gtgtccatca
    10141 taacaccgct gacttttttc tctctgcggg gaatggcgtt taagatctgc tcattctctt
    10201 tctcttccgc acggagtctg cgctcttcct cacggatccg tcgttcctct tcccggatac
    10261 gacgggcttc ctctctctgg gaattacgtt cctcctgttc ctgtctgcgg atctgtgcat
    10321 tttcccgtct gcgcactgca tcctctctgg acagttcccg gatccggtca ccaccgtttc
    10381 gcatgctgtt taagaaagaa cgctccgtta ccaggatcag actgatcata gaacacaata
    10441 agaccaccag aaccgttcct accgtctcca gataatgcag caacagatag cagatacttc
    10501 ctgccagaat acctccgccc tttttaccgg tactgcagtt tgtatataac agtttgatat
    10561 catactgttc catagagccg gcagtcctgg cgatcagatc acagatcaca ccgagcatca
    10621 gaaacaatac gacacctgca atcattttgc gtacagcggt agggttcccc tcattggcga
    10681 accagaaagc cacagccaga aatatcacaa taggtgctat atatgcagta aatccaaaga
    10741 taccgaataa gaccccgctg atcgcattgc cgaccgggcc gataatacca aaattacaac
    10801 aaaataatat caccattacc acaaacaaaa cgatcagacc gatctcatgg aacagttcac
    10861 tgtcctgtgc tgttctctgc acctgcctgc tgcggctgtt tgtggtactt tttctcgtcc
    10921 tattggaaga tgatgttctt ttgcctgatg aagatgccat gtcatcatac tcctaacttc
    10981 attatagtca taattagaag tatatcattt ttccatggcc ttgtcatccc tttttctgtt
    11041 cccttcgatc atcttccgaa gcctgcagat ggcccggtcg atacctccca cttcgtcaat
    11101 aataccgtgg ttcaccgctt cttctcccac cagaatgctt cccacatcct tcgttaagaa
    11161 tccggtctcc atcatgagtt cttccagttt tgtccggctg attttacagt gtttgcatac
    11221 aaagcccgtg atccggtcct ggatcagttt aaaatattca aaggtctggg gcacaccgat
    11281 caccatgcca ttcattctta ccggatgtat caccatggtt ccagtgggta cgatgaagga
    11341 atagtcacaa ctcaccgcaa taggcacccc gatggaatga ctgccaccca gcaccaggga
    11401 tacggtgggt ttgcttaaag atgccagcat ctctgcaata gccagtcccg cctccacatc
    11461 tccgcccatg gtatttaaaa gtaccagcac accgccaatt tcttcactgt cttcgatggc
    11521 tgccagttgt ggcaggatat gctcatattt cgtggtcttc gaattaccgg agagattatc
    11581 atgcccttcg atttcaccga taatggtcag cagatggatc ttttccccgg atgccgcatc
    11641 cgacagcgtc agttgtcccg ttttttcaat tctttcgtct ctgtgttctt ccgcctcggt
    11701 cttcggcgct tcattcttgt tctgattgtt atctttctga ttctgcatgg cctgtttcct
    11761 cccagtttca atgattttat gattttgaaa atttaaataa caaaaaaaga cgacacaaag
    11821 gcattccacc tctcgtatcg tcttttagta tgtccgtcaa tccaacattt cattaggaaa
    11881 ccaacagatt tttaatgatc aaaatcatcg tcatccgcta ccggataatc aaaatcactg
    11941 tcatcgtcat tatccagctt ataatccagt accttaggca catgcttttt cggcaggggc
    12001 agtggatttt cgatcagctg tattttttcc gtttctacag gctcttcctg gaaatccgct
    12061 ggctcttcct ccgggaattc ctcggtctcc agatcctgta ctttcagccg ggatctgcgt
    12121 ttcaccgttt ccgtactctc cctttctttt tcctgcatca tggtcacata gggaatcagg
    12181 ctttgtactc cggctccaat gagaagataa cgaagccgga catgcatcca cattccctga
    12241 atatccgggg ttgtgatctg aaacgtcatc agtaactcca acacaatcca caaaaggatc
    12301 cagatactct gacgttctct gcgtttctga caccagaaac caaatacacc gagaatttgc
    12361 attgttacca ataaaaagat cagaatcttg ccggctacca cagtatccgc tccggtcgtc
    12421 atagtaccgt cgtaaattgc aaccggccag gaaaaagcag acggttggaa cagccgtccc
    12481 catgcagtga gaacatttcc cataagtttc ccgctgacac aggcgtctac tgcgattgaa
    12541 acaaagaatc cgactgcgcc tccgaaagct gccagtaatg ctgcccacat tctatgtaaa
    12601 aatcccacag tttcctcttc cgctgtctcc agtgcaaaaa ccgaaagtat cggaagatac
    12661 agtaataaac ccgtgatatc cagataggtc agcactccca ttgccacacc agcgataaaa
    12721 aaactgagaa tcgggcggac gcccttctgt tcaccttttc gtcggaaggt attcagtgct
    12781 gccgcaacca gacacaggcc tatcatccat aacatctgat aaagttcctc cggtcccaat
    12841 actactacct tccagcacaa tggccagagc attccaaatc ccagcgccgt aactgccgca
    12901 gccggtccag ttaatcggcg gaccgcaaaa taacaaagaa tccagccaag aatctgtaag
    12961 cccgcctgga atccgagtgc cacggtgatc ttattgccga ggagcagcaa aagcccgtgc
    13021 agtatctgca gatacagata aaccgcgcca tgtactacct gtgggatctg cgtctgtgtt
    13081 gtcactcgga ccgtatcaaa ccagacatcc tccgccgcta catccagatc catcagctgg
    13141 atttcacgaa tcctcaggac aatccccgcc gctaccaaca gcactgtgag gattccctcg
    13201 accacaagtg cagtctgccg attatctacg tttgtttttt tctctctgct cttttgcagt
    13261 cctcttgaca gcagaaaagc aacaagccct gtgacagcaa gccatacacc tgctatcaca
    13321 aggccgatcc attgggattc ctccggagcg cttggcatag tgaccatcat cgctccggtt
    13381 cccgccataa ttgcagaaag taaccacagt attacactta ttacattctt tttataattc
    13441 attatttcag taccttttcg atgttatatc tgggacgatg ttttacctca atgtaaatct
    13501 tgccgatata ctggcccacc agtccgatgg ccattaactg aataccaccc aggaaccaga
    13561 tggacaggat cagggaagtc cagcccggta ccacatgtcc ggtacaataa gagatcagcg
    13621 cataaatagc tgccagaagg cttaaggcca caatgatcat accggcaccc aggatcatac
    13681 tgataggctt cacactgaag gaagaaatac cgttgaatgc caatgcaagc atcttcttca
    13741 gaggatactt ggactcaccg gccactcttt ccttacggtc atagtataca cagtccgtct
    13801 gataaccgat caaaggcatc atacctctta agaacagatt ggtctcctga tacttggaga
    13861 actgttctac cgcgcgcttg gacatcagtc ggaaatccgc atgattgtaa acagtcttga
    13921 cccccatcat ttccatgact ttataaaatc cctgcgccgt ggtacgtttg aagaaagtat
    13981 cggtcttacg ctccttacgg acaccgtata cgatatcaca gccgttgtgg aacttatcga
    14041 tcatttcctc gatcacagct acatcatcct gcagatctgc atcaatggaa accgtcacat
    14101 cagacagatc catggcagtg attagtccgg cagtcagtgc gaactgatgt cccacattgc
    14161 cggccagctt tacaccgcat acctgcaccg gatgtgcctg atgctcttct tcgatcagct
    14221 cccaggtacg atccttactg ccatcattta caaacagaat aaaactgtct tccgcgatct
    14281 ttcccttatg gatcagatca tccagcacac ccctcagtgc ctcggaagca atctttaaca
    14341 cctcttcttc attataacaa ggaactacaa ttgcaagttt ctccatgctg ttacccctaa
    14401 cctttcaatt tattcgtccc agttatgata tactgcctga acgtcatcat catcttccag
    14461 cagttccagg gttctctgta agttcttcac tgcagtctca tcggtcagtg ttacataatt
    14521 ctggggaatc atggtaactt ctgcactgat catgggaatt ccggcatctt ccagagcctt
    14581 gcgtacctct tcgaagttat cgggatcagt caggatctcg aagctgtcat cctcttcgga
    14641 gaaatcttcc gctccggcat ccaaagcgat catcatcaga tcgtccgctt ccatatcaca
    14701 ttcttccttg tccacgatga tctgtccctt cttatcgaac ataaaggata cacatccggg
    14761 agtacctaca ttaccctgtc ccttggtgaa tgcgcttctt acgttggcag cggtacggtt
    14821 ggtgttgtcg gtaagtgcat ctacgatgat ggcgataccg ttggggccgt aaccttcgta
    14881 agtcacatac ttatagttta cattgcccac atctccggct gccttcttga taccgcgctc
    14941 gatggtatcg ttgggcatgt tgttggcttt tgccttggca ataacggttg ccagtttgaa
    15001 attgttggcg ggatcgggac cgccctcttt tacagcgact gcgatctcac ggccgataat
    15061 ggtaaaaatc ttaccctttg cagcatcgtt cttttctttt ttatgcttaa tattagcaaa
    15121 tttggaatgt cctgacattt ttgttactcc tttgaactta aagtttcttt ctctttgtat
    15181 gcgtagtttt tacgcaataa cgctctttct atcatagcat tattttcttt tttcttcaag
    15241 cactgattgt ccgtcttctg cattctgcgg tgttttttcc tccggaagct tccggaccaa
    15301 cacacttcct atcatttttt tgaccacgga aaggatttta aagttgtatc ccttatagtc
    15361 cacatcgaac tgctcgtccg gttccgggat ccggtccagt ctggagatca ggaaaccgtt
    15421 caaagtctcg aattcttcct cgtcgaaggt aatacccaga agctcttcca cttcctttaa
    15481 cggagtcttg ccttccatga catattcgcc cttgccacgg ttcttgatgt attcctgatc
    15541 ctcatcatac tcatccatga tattgccgac gatctcctcc agaatatcct ccatggcgat
    15601 cagtccgtcg gtctgtccgt attcgtctac cacaatggcc atctgcagct tctgggactg
    15661 catttccttg aacagttcat cgatattttt cgtctgcggc acaaaataag gctctctgag
    15721 cagcccgtcc agttttgcga taggcaggtc cagcttgtca tcactgatat ggaaccgcat
    15781 ggcatccttc agatgcagga taccgatgat atgatcaata ttctcctcat acacagggta
    15841 acggctgttc ttgcctccca gcatggaagc aatggcatct ttcagtttcg tctcaccatc
    15901 aatggccacg atacttccac gatgtgtcat gatatcctgg gcttccttgt ctccgtattc
    15961 gaaaatgttg gagatcatct ccgcctcact ggcctgaatg acaccctgct catgaccttc
    16021 ctgcaccata ttgatgatct cttcctcggt cacatcattt tcatccgtat tgttgcggac
    16081 accgaagagg aacaggattc ccttggctgt cagattcacc agccccgtca ggggtgccag
    16141 cagtctggtg ataatataaa tcgggtcaat acaggcatac gcccacttct ccggctgtct
    16201 ggatgccatt ttccggggaa tcagcacacc gaaagtcaac aggatataga tcatggcaat
    16261 aaaagacaat accgtggcaa tccctgaaag cactcccgta ggcacattct ccagagacat
    16321 gcctcccaca ttgatcacat gaagtccctt gtcaatggtc tgcagccaga tattcagata
    16381 aaaagctccc attaaaatat tgatcagcgt aataaccagc tgtaccgtat tgatatagat
    16441 ccccggacgg ctgatcattt cactcagccg catggaccgg cgatcctttt cttcctctgc
    16501 tttatgtagg atctcctttt cattcagatt gtcgatggcg gcatcaaatc catagaaaaa
    16561 catatccacc agcaacagca ggaaaaagag aaaaatggcc acaggcccac tcatgtccat
    16621 attttatttc atcctttcgt attttatcct attttcggat acactatgca tcgggctatg
    16681 tatccagctc caggctgact ttcagtgccc ggttcactct ccgcataatg tctccgtcca
    16741 gatggcatac cttatctttc agcctctttt tatcgatggt gcgcagctgt tccagcagca
    16801 ctacggaatc tttatccata tcgtagcggc gggcattcag ttcaatatgc gtaggcagct
    16861 tcgccttgtt catctttgag gtgattgcag cgcagatcac ggtgggactg tgtttgttcc
    16921 ccacatcgtt ctggatgatc agcaccggcc ggatgccgcc ctgctccgag ccgataaccg
    16981 gtctcaaatc tgcgtaatag atatctcctc ttctcatttc tactccttca tcagcgattt
    17041 aacataagta ttgccgattc ttccggagtt atacgaacac tttagatccg tcagaaattt
    17101 tcaaaataat cttttacttc tgtgatctca ccatttcgac ggtagactct gggaattcgt
    17161 tttcccagtg tgcagacaaa ctcataattg aatctgccgg aaagttctcc cagttcttcc
    17221 gccgtgatcc gctcctgacc atccgcgcca aggagcgtca ccttatcgcc ctccatggct
    17281 ccgggaattc cggaaatatc caccatgaac tggtccatgc agacccttcc gaggatgggg
    17341 gcctttttcc cgtggatcag cacgtagcct tttcccgaca ggcttctggg atacccgtct
    17401 ccatatccta cagggatggt ggctactctg gtatcttttg ttgcggtaaa caatccaccg
    17461 tagctgacac tctgccccgc atggatcgtc ttgcagtaaa tgatatgact gtagagagac
    17521 attgcagccc gcagaggtac aatatcctta cttacttctt cggaaggata taaaccgtag
    17581 gtcgtgattc ccgcccgtac catatccatg ttggcctggg gcatttccag aattgccgca
    17641 ctgttggaac aatgctttac cggaatctcc agtcccagtt ccgactgtat tctatcaata
    17701 aaattctgaa acagttccag ctgatggttg gcagaagtct tatccgcctc atccgatttc
    17761 gcaaaatggg taaagatgcc ttccacttcc agtccgggat gttccatgac aaattttaca
    17821 aaatccagcc cttcgtcgtc cggagtgata ccgatccttc ccatgccggt atccactttg
    17881 atgtgcactt tggctttctt accggccttt gctgcggcct ctgccagttc ttccacggta
    17941 tccctgcgat acactgccgg gcggatctct tcccggatca tttcctcata acaataagga
    18001 aaagtatatc ccaggatcag gatcggtttc ttcacactgg cttccctaag gacatgtgcc
    18061 tcctcgtagg tagctgccgc atagccgaac ataaaatcca catcctccag cattctggca
    18121 atgggaacac cgccgtgtcc gtaaccgtcc gtctttatga ccgccagtat cttcgtctcc
    18181 ggggcaatat tttccttcat atggatcata ttctcccaaa tggcatccag atctatttcc
    18241 gcccatactc gttggtagga attcatcttt tcctgtagtt tgcttatcat ttcttcacac
    18301 tttctttctc atcatagtct ttcatcaggc aatccgcaat atctcccgcc attaccccat
    18361 actccgtatg gagctttgcc gccagctcac cggctctgcc atggagatac acaccgttaa
    18421 cagctgcatc aattgcattc ataccctgag ctaaaagtcc caggatcaca cccgtcagga
    18481 catctccgct gcccgcagtg gccataccgc tgttgcctgt agtattcaga taaaattctc
    18541 cctgttcttt acatacaatc gttcttgcat ccttggcaac cgccacaccg tgaaaccgtt
    18601 ctgccagttc tctgccacac tccggaagct ccttcttgca ttccgggatg gtcttcgcaa
    18661 gcagtctgga caattctccc acatgtggag tcagaatgat aattctgcct tctgccccct
    18721 gggtacgcag cttctctgcc aattccttac cgttttcttc cgccagaaga ttcagtgcat
    18781 ccgcatccat cactaatggt tttctgctct tttccactac ttttttcagg cattccagtg
    18841 cctgttcctc tctgccgatg ccgggaccga cagcaataac atccgcccat tccatgctct
    18901 cggacagctg tctgcaggat ccgtacaaag cctccggaat tccctgctgt aagatctgcc
    18961 ggttctcctc tgcggtgatt acttttacca taccggctcc ggtacgatac gccgctcttg
    19021 ccgccaggat tgctgctccg gccatgccgt tgctcccggc caccagcaaa gctttgccga
    19081 aggttccctt attcccggag gatgttctgt ccggcagatg ctgttcacag ctgtcataag
    19141 tatacatctc cgggctctgt cccagaaagc tctccggtcc gatccccaca tttgcaatgt
    19201 gtacctgccc ggcataatcg gttcccggat acattacaag gcctctctta cagaaaccaa
    19261 aagtaatcgt tgcatctgcc aggaaagcac aaccaagcac ccgtccggtg tccgagcaga
    19321 tcccactggg aacatccagt gccagcttaa atccttccgc cacattcatc tcttctacgg
    19381 cttccgcata gatgccttcc accgggcggc tcaatcctac accgaacaag gcatctacaa
    19441 tcacattata ttcatctgcc tgcgtattac tgtctgtctt caccggaaaa tgttgcaggg
    19501 tctgccactg gctcttccat tgctctgtgg ctttctccgg atctcccaca agtcgtaccg
    19561 ctaccgtata accgtccgcc gcaagaagtc tcgccaaagc cagaccgtct cctccgttat
    19621 ttcccatacc agcaaaaatg agcacttttg tccgttcctt cacggcatca aagcgctctt
    19681 tcagaaaatc ctccgcagcc agagccgctc tttccatcag aaccggcccc gggatgccga
    19741 ggtactctat ggtattttta tcgtattgct tcatttcctg tgcagttacc agatatctca
    19801 tactattttt gctccggatt atatttcatg tcaaacagat tgaccacttc tgcaccaaaa
    19861 atatcatgca tgtagttgta gatctcctga ttctgctgtt ccatctgctg tttttcgatc
    19921 agacgttttt tcagttcgat acggatcgca cccacccact ccgcagtctc tttgatctgt
    19981 ttgtcattat ccttcataat atcatagcag cagtccatag taaacatcat aagctgaaga
    20041 tttattcttt cgatctctct ggggatatct tttaactcat cttcgtatgc ttccagcttc
    20101 tcattgcatt cctcgatcag gcgcttatga tcctcgatct gtttgttcag cttactgttt
    20161 tcgccctgct gttccgcctc atccaccata gggacgatct ctttcatcag cttctttttc
    20221 agattccgga tatccttcga ctcggtattg agcttacctt gtcgtttcaa cagctgattc
    20281 agggcatctt ccgtttcttt tagcggaacg gaaccggtct tattcaacaa ccgataccat
    20341 ttattatcca gtgtgagtac gggcagatgc tttcccgtca atgcactttt gaaagcagcc
    20401 tgtcggtcct gtgcattttc ttctgcgtta ccgttgccca taatatcact cctttgatgc
    20461 cttacttcat gttgtattat ttctcggtgc gctgaaatca taaaagcggc tgcttcgcat
    20521 ccgtcatcgc cttgcgactt ttccttttat tcattttcag acactccgcc tatgaaataa
    20581 gcaatcatac gcttgcttat ttcatgtctg aactgtaacg gttaatatta taggatagct
    20641 tcattaagct gtcggacaga tgcacgttct ccacctgaat tccctcggga acattcagat
    20701 ccacatgagc gaaattccag atagcacgga tcccgttagc caccagctta tctgccactt
    20761 ccacagcact ggtctttgga atcgtcagca cagcgatatc gatgtcattc tcccggataa
    20821 aggattccag ctgatccatg ggcatgacct tcacaccgcg gacatcctta cccaccagtt
    20881 ccgggttcgc atcgaacatt cccttgaaga taaatccacg acgttcgaag ttcatataat
    20941 tgcccagcgc tcttcccaga ttgccggctc caatgatgac gaaattatgc tgcttatcca
    21001 gtcctaagat cttaccgatc tcacgataca gagattctac attatatcca tagccctgct
    21061 ggccgaaacc gccaaaatta ttaaaatcct gacgtatctg ggatgcggtc accttcatca
    21121 gtccggaaag ttcctgcgaa gagatgcgtt ccacaccttc atccttcaat tcccccaaat
    21181 atcgaaagta tctgggcagt ctgccaatca cagcctgtga aatttctttt acttccaata
    21241 ctttcacctc cgttttttta gtatacacca tatgttaaaa acttgtcaat gaattgacag
    21301 gttttcttct tttcgttaca atgaaattta aaaagcccgg tctccgtggg ttggaaataa
    21361 aggatactta ctaccatgat attatcttgt cagaatattt caaaagcatt tgtagaaaat
    21421 caggtattga aaaatgtctc ttttcatata gaggatcatg aaaaagctgc catcgtgggc
    21481 atcaacggtg ccgggaaaac gacactgctc cgcatcatcg taggggaaat gactcccgat
    21541 gacgggcagg tggttctggc gagggacaag accctgggat accttgcaca gaacagcact
    21601 gtggacacct ctcacaccat ttacgaggaa ctgttatctg tcaaggcgga tctgctgcgc
    21661 ctggaggaaa agatccggga atgcgaaaac aatatgaagc atgccgaagg agatgcgctg
    21721 gaagacctga tgaagcagta cacctctctc acccatgctt tcgagaccgg cggcggctat
    21781 ctctaccgta gcgagctggt cggtgtgtta aagggactgg gttttaccga agatgaattc
    21841 tccaagccgg ttgccacgct ctccggcggt cagaagaccc gtgtggcgtt aggacggctt
    21901 ctgttacaga accctgatct cattatcctg gacgagccga cgaaccactt agatatgaat
    21961 tccatcgcct ggttagagac ttatctgttg aattacaagg gagccgttct catcgtttcc
    22021 cacgaccggt attttctgga ccggattgcc gggaaagtca tcgagatcga ccagtccaag
    22081 gctacaacct ttatgggcaa ttactccgat tatgccgtaa aaaaggaaca gctgcgcgtt
    22141 gctgcctgga atgcttatat gaaccaacag cgtgagatca agcatcagga agaagtcatt
    22201 gaaaaattaa agtccttcaa ccgggaaaag tccatcaagc gggcggaaag ccgggagaaa
    22261 atgctggaca agattgaggt catcgaaaaa ccctccgaag tccgcacaga tatgaagctg
    22321 acactgatgc cccggatctt aagtggcaat gatgtgctga ctgtggagca tctgtccaaa
    22381 agctttgact cccataagct ttttaccgat gtcaatttcg agatcaagcg cggcgagcat
    22441 gtggcgatca tcggtgataa tggcagcggc aagaccactt tgcttaagat cttaaatggt
    22501 ctcgttcctg cagatcaggg taccttccga ttaggttcca atgtggagat cggctattac
    22561 gatcaggagc atcatgtgct ccacagcgag aagacattgt tcgaagagat ctcggacgat
    22621 tatccctacc tgaacaatac ccagatccgc aatgtactgg cggctttcct cttcaccggg
    22681 gaggatgtgt tcaagcgcat cagtgatctg agcggcggtg agcgcggacg tgtctctctc
    22741 gccaagctgg tactctccaa cgcaaacttc ctcatcctgg atgagccgac gaaccactta
    22801 gatatcatgt ccaaggagat tctggaggat gccctgaacg gttacgaagg aacgatattg
    22861 tatgtctccc atgaccgtta ctttatcaac cgtaccgccc acaggatcct ggatctgacg
    22921 gagggacaat tcgtaaatta tgttggaaat tatgactatt atctcgaaaa gcacgacacc
    22981 gtgatggctg caatagaagc cagtgttcca cagagtgctg acgcagataa cacagttgcc
    23041 gctaaggttg cagaatccga agttaagctg gactggaaag cccagaaaga agaacaggca
    23101 cgactccgca agaaagaaaa cgacctgaaa aaatgcgaag aacagattgc cagactggaa
    23161 gcccgtgtct ccgagattga caccgaaatg tccgatcccg ccatcggcac ccaggtggca
    23221 aaattgcagg aactgtccaa agagcaggcc gcctgccaga agcagctgga aaaattatat
    23281 gaacagtggg aagaactggc agagtaaatt gagagttagt gaaatacatt ggcaaaatcc
    23341 acgaactccc ggagcatg
//
LOCUS       DAWEJW010000037        23252 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_896_length_23252_cov_58.086563, whole genome shotgun sequence.
ACCESSION   DAWEJW010000037 DAWEJW010000000
VERSION     DAWEJW010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 23252)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23252)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23252
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_896_length_23252_cov_58.086563"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4310..4409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8491..8590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5357 a         6283 c         4848 g         6564 t
ORIGIN      
        1 gccggcggta ccggttcctt catcgaccag atggccagtc tgattcagac ggacgcttcg
       61 ggcttaaacg aatatgcgaa aaattataaa tccctctaca ccattgctgc ccgctgtggt
      121 gtatttgcca aaaccgatat ccagcctctg atcaacgaag gtgctaccaa ggaagacctg
      181 tctgcttcta ttttccaggc agtggtgaac cagacaattt ccggtctggc ctgtggtaag
      241 ccgatccgcg gtcacgtagc tttcctgggc gggcctttac atttcctgtc tgaactgaaa
      301 gctgccttta tccgtacctt aaatctggat gacgagcaca tcattgacgt ggacaactcc
      361 catctgttcg cagccatcgg ttccgcacta aatgcgaagg aggaagtctg catcagtctg
      421 tctgacatgg tgaaaaagct gtcctccgac atcaagatgg agttcgaagt agaacgtatg
      481 gagcctctgt ttgcggacaa ggctgcttat caggaattcg tggagcgtca cagcaagcat
      541 cacgtgatca cgggagactt acagtcctat cagggcaagg ctttcctggg tatcgatgcc
      601 ggttccacca ccacaaagat cgctctggta ggtgaagatg gatccctgct gtactctttc
      661 tattccagta acgacggaag ccccttgaag acagcgatcc gttccctgaa ggagatctac
      721 tctctcctgc ccgaaggcgt ggagatcgta cgctcctgtt ccactggtta cggcgaagcg
      781 ttgatgaaag ctgcttttct gctggatgac ggtgaggtag agaccgtggc ccattactat
      841 gccgctgctt tctttgaccc cagcgtagac tgtatcttag acatcggcgg tcaggatatg
      901 aagtgcatca agattaaaaa ccagaccgta gacagcgtac agctgaacga agcatgttcc
      961 tccggctgtg gctcctttat tgagaccttt gccaaatccc tgaactatac cgtgcaggat
     1021 ttcgctcagg ctgccctgtt tgcagaacat cctatcgatc tgggtacccg ttgtaccgta
     1081 ttcatgaact ccaaagtaaa acaggcgcag aaggaaggcg cttccgtagc agatatctcc
     1141 gcaggacttg cttactccgt tatcaagaat gctttattta aggtaatcaa agtctccgat
     1201 gcttcagagc ttggtaaaaa tatcgtggta cagggcggta ctttctacaa tgatgctgtt
     1261 ctgcgcagct ttgagaaaat tgcagactgc caggcgatcc gtcccgacat cgccggtatc
     1321 atgggtgctt tcggtgcggc tctgatcgcc agagaacatt acgaagacgg ttatgtgacc
     1381 acaatgctgg attatcagaa gatctgcgat ctccagttcg agaccagcat ggcgaaatgt
     1441 aagggttgta ccaacaactg tcgtctgacc atcaacaaat tctccggcgg ccgtcagttc
     1501 atctccggca accgctgtga acgtggtatc ggtggtcaga agaatgccca caatgtaccg
     1561 aatttatacg aatacaaatt acatagactg ttctcttatg aatctctgga cgctgacaag
     1621 gctcccagag gtgtagttgg tatccccaga gtgctgaata tgtacgagga ctatccgttc
     1681 tggttcacct tcttcaccga actgggctac cgcgttgtgc tctctcccag ctccaaccgt
     1741 aagatctacg agcttggcat tgaatccatc cccagtgaat ccgagtgtta tccggcgaaa
     1801 ctggctcacg gccatgtgac ctggctgatt cgtcagggaa tcaagttcat cttctaccct
     1861 gcgctattct atgagcgcaa cgagttcgag gatgccaaca accattacaa ctgccctatc
     1921 gttacttctt attctgagaa tatcaagaat aacgtagagg aaatcgccgg cggtgagatc
     1981 actttccgca atccgttcat gtcctttaga gatcttggta ccgtgacgga tgcgctgacg
     2041 gaggaattca ccgagattcc cgccgcagag atcgccgcag cctgcgaaaa gggttggaag
     2101 gaactggcaa atgcccgtca cgacatggag aaaaagggcg aagagactct ggaatactta
     2161 cgcaaaaccg gcaagcgcgg tatcgtgctg gcaggccgtc cctatcatgt agacccggag
     2221 atcaaccacg gtattcccga gctgatcaca tcctacgata tggcagtact gacagaggat
     2281 tccatctccc atctggcaaa accggaacgt cctttgattg tatccgacca gtggatgtac
     2341 cattccagac tttacgctgc cgcaagctat gtaaagacgg tggagaatct ggatctgatc
     2401 cagttgaatt ccttcggctg cggtctggat gccgttacca ccgatcaggt gaacgatatc
     2461 ctctccggct ccgacaagat ctacacctgc ctgaagatcg atgaagtcaa caacctgggt
     2521 gcggcccgca tccgtatccg ttccctgttg tctgcaatcc gtgtccgtga aaagatgggc
     2581 aagcaccgca acatccgttc ttccgctatc gagaaggtac cgtttaccga agagatgcgt
     2641 aaggactata ccatcatctg tccgcagatg tccccgatcc actttgagat cttgcagcct
     2701 gcgttcaatg cctgcggtta caatttcgtg gtattggaca acgataacaa gcactctgtg
     2761 gatgttggtc tgaaatatgt taacaatgat gcctgctatc cttccctgct ggtagtaggt
     2821 cagatcatgg aagctctgct gtccggtaaa tatgacctga acaagacagc catcatcatg
     2881 agccagaccg gtggcggatg ccgtgcgacg aactatatcg gcttcatccg tcgtgccttg
     2941 aagaaagcag acatggagca gattcccgtt atctccctga acctggctgg tatcgagagc
     3001 aatcccggct tccatctgaa tgcggatctg ctgttacgtg ccgctgtggg tgcggaattt
     3061 ggtgacatct tcatgcgttg tgtatatcgg atgcgtcctt atgaagcaac tccgggttcc
     3121 gtggatgctc tgcacaaaga atggcttgcg aaggtacaga aatttgtctc cgcgaagcat
     3181 atcagcattc cgaagttccg caggatgtgt accgagatta tccgggattt cgatgcggtt
     3241 ccggtactgg atatcaagaa gccccgtgtc ggcgtggtcg gtgagattct ggtgaaattc
     3301 tctcctgcag gcaacaatca tctggtagag ctgctggaga gcgaaggtgc ggaggctgta
     3361 gtacccgacc tgatcgactt catgctgtac tgcttctaca accagatcta caaagcagaa
     3421 cacttaggta ccagcaagaa gactgcaaag atttctgcgc tgggtatctg ggccatcgaa
     3481 catatcctcc gcggctctgc cgtgaaggct tttgaagaaa gtaagcattt tgatccgcct
     3541 accagcattt acaagatcgt ctcctatgcg gaaccgatcg tatccatcgg caaccagacc
     3601 ggtgagggat ggttcctgac tggtgagatg gtggaactga tcaaggaagg cgtaccgaac
     3661 atcgtatgta cccagccttt cggctgcctg ccgaaccacg tagtaggtaa gggcgttatc
     3721 aaggctctgc ggaaggcata tccttcctcc aacatcgtag ccatcgacta cgatcccggt
     3781 gccagcgaag tcaaccagct caaccgaatc aaactcatgc tctccaccgc ccagaagaat
     3841 ctgaagaagg gtgaaggaag cgcggaataa aacatttata gtctgaaacc actgcccgcg
     3901 gcaagggatt ccattggaat tccatgccgt gggtgttttg tattcagatc tcttttccca
     3961 aacaaacatc ttctcaaaat ctcccctgtc atgttataat acactcagat atgcttgtgc
     4021 cttcaaaagt gcccgctcta ttgcttatct gcatgtccca caatgttcat ttatctccat
     4081 tttcgctaaa agctgttttc cggctatttt agtacataat ttctcatatt ttttcttttt
     4141 ttgtatctat tttacccttt ttctttattt tgatacaatc atcccttttt ccacttgaaa
     4201 attagacttt tttgtatttt tttctggtat tttatcctat ttacaccaaa ccattttgaa
     4261 aggagttttt tatgattcag aatttagaca ctcgccttaa taaattacan nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc atgattggcg ataaccaaac gggcacaaaa
     4441 aaagaccggg cgtgtatcga cctccagtca ttagattgat tctgtttcta acttttgggc
     4501 ggtacagctc cggttcttta aggtgagcaa gctctctttc acctgcaatc tcacgatgca
     4561 tttgcgctgc ggttctcaat gtattgttgc aagacttcgg tgacttcctc cggtgccagg
     4621 ccggcatcct cgatcaggtc acttactgcc tccagttctt tgcgcctctt ctgctcatat
     4681 aattcttcca gttcccgttc ctcggattca atgcggatat tcagcgcatt gatcaactcc
     4741 tgttttgcct ggatcttttc ctccagggat tttctctgtc ctcttggcat atactgtgtg
     4801 ctcctttcct tatggcaata ctatcgaaaa ggccattcat cagggatcac aagcgtagca
     4861 taagctgccg tgatcagtca tgaatggcct gatatgttac agtatatgga caacattgta
     4921 aaaatatacc atatgtctgt taatattata ccgctataat ttttgtccta tcggaacaag
     4981 cgtccacaga tatttttctt tgctcacatc aaatctgtcg gatctagccc aggttgtcta
     5041 ctgacgcctt cagcttgtcc agtgcaagct ctagagttgc tctggggcag gcaatattca
     5101 ctcgctggaa gtctgctcca ctgggtccga acattgctcc gccatccagc cagagtcccg
     5161 ctttctctac gataaactga tccagttcct tgccggaaat cccaagttca ctgcaatcca
     5221 gccataccag ataagtacct tccggctcta ccagatgaat ctgcggaagc ttctcctgca
     5281 gatatgctcg caggaaattc agattctcaa gcagataagc tctcaactga tccagccact
     5341 cctgtccgcc acgatatgca gcctcgcagg ctaccgctcc caacgcattc ggctcatcgt
     5401 atccggtctt gtcgatttcc ttctggaacg cttcccgcaa tgtctcattt gagataaaaa
     5461 tattggaaat ctgtaagcct gccagattga aggtcttact gggtgcggtg caggtcacgg
     5521 tgaactccgc aaaacgggga tccacattgg caaacaccgt atgctcaaat ccgggatata
     5581 caaaatcatt gtggatttca tcactgacta cgatcacatg atggcgcagg cagatctctc
     5641 ccagctgctg cagttcctct ctggtccata ctctgcccac cgggttatga gggttgcaca
     5701 gaataaacag ttttatatgt tcctgcacga tcttgcgctc gaagtcttca taatctatgc
     5761 tgtactgcga ctttccttca gttacccttt tttcgtatac caatgtatta tcaatcaccc
     5821 tacggtcatt gtccctaatg atattggtaa agggataata caccggattt tggatcaaga
     5881 ccgcatctcc cggctttgtg aaagctttta ccgctgcacc cagtgcaaat accacaccgg
     5941 gggtcttcat gatccactct ctcttcgtct tccaattatg gtttttttcc atccaattgg
     6001 ttatggcatt ataataagca tccttaggct gggtgtatcc gtagattcca tgatctaccg
     6061 ccgcgtgaag gcttttcgta atctcctccg atactgtaaa gtccatgtct gcgacccaca
     6121 gtggcagcac atccgcaggc ttgcctctct ccacggcata gtcgtacttc aggcagttcg
     6181 ttcccttacg atttataatt ttatcaaagt tgtatgtagc catacgaatc ctcttacctt
     6241 tcgacaacct gcaagctttc tgcctgtggc cccaagttgc aggttaaatt tttctgtcaa
     6301 tcatacctat tctaccagga ccttctgcac gtcctgactc gttgcgtctg ttgtgggact
     6361 tccgtgaaac tttccgcgga actggctaaa ttactatgta taccagtcaa ccccactcag
     6421 gcaaaggcct gctccaggtc tgccagcagg tcatcaatat tctcaatacc aacagacatt
     6481 ctcaaaagct tttcatccac acacagagct tccctgacct ctacaggtac atctgcatgg
     6541 gtctgcttca tgggataggt cagaagactt tccacgccac ccagactttc tgcaaaacgg
     6601 atcagcttta cgctctccaa aattttaaca gctttctccg cagaattcaa tcggaaagaa
     6661 atcattccgc catagccgct gcactgtctg tcaatcagct ccttatccgg tctggaatcc
     6721 agtccgatat aatagacttc ttccacttcc ggtctctgtt ccagccagtg tgcgatggcc
     6781 agagcattct tctggtgctg ttccatgcgg actgccagtg tcttgatacc gcggagtaac
     6841 aaccagctgt caatcgcaga cagacaggct cctgtagtct tgtagatatt atagagctgg
     6901 tcatacagag cattatcctt caccaccaga aaaccggaca aagtatcatt atgtccttcc
     6961 agatatttgg ttccactgtg taccacgata tcagctccta atgtcaacgg cttctggaaa
     7021 taaggagaca gaaatgtgtt atccacgatc aacaggcatc cattctcatg cgcaagatct
     7081 gcaattgcct gaatatccgc tactttcatc atcggattgg acggtgtctc cagatagata
     7141 gcttttgtct ccgggcgcaa cgctgccttc accgtatcca cgttggaagt atctacacaa
     7201 tccacggaaa taccattctt gcgactgatc atgttccaca gtcttaatgt tccgccatac
     7261 agatcatcgg tagcgatcag atgatctccg ggagaaaaga tttccatcat tgcagtgatt
     7321 gccgccattc ccgtagaaaa tgccagtgcc tctgttccct gttccaacag agcaaccgtc
     7381 ttctgcactt cgtctctggt ggggttgctg cagcgggaat aatcatatcc cgtactcttc
     7441 cccaggcccg gatgcgcata cgccgcattc tgataaatgg gcatgctgat ggcgccgaac
     7501 ggatcctttc tctcattact tccctggaca catatggtct ctgctgcaaa tgccatcttt
     7561 ttctcctcct atgtctatgt atactatatt attcccttta tatttcctac tgttccagta
     7621 ggtatagtat atatttatca tagtacatag aaaaagtcaa gtttctattc catatttacc
     7681 aataaagttt catttctttc tcagcttcct gctctgagat attcagtttc agtctcacct
     7741 tttgtaaagt ttcttcataa ctggtattaa aatccttgca cattataata atcccttgcg
     7801 caactccctg agaaatcccc tgcgaaattc cctgtgcaat cccttttctt gtatactgat
     7861 caaataattc acacaccctg atctcatcct cctctttgat tcccatttct tttaatgcat
     7921 ctccggtatc atcaacattt gtatctccgg ataatacccg tagcatctta actgttgctt
     7981 ctttatgaat cactttccgg tccgaccgat agccatctcc ctctgcaaga taatccacga
     8041 tgattctcat atcactttga aaaagctctc gtatttctct gggtaaatga cgcatttccc
     8101 aaacatgaag ttgtacatca tctacatact tccatataat atctgcagtc gcccctcctt
     8161 catcaaataa ccggtgcaaa ctgcatcccc ctttccacct gctctttccc cagtataaca
     8221 ctatttctat cacaggatat aggctcggat tttgtttttc atattgtctg cggtattctc
     8281 cacctgcata cccggccttg cgcaatacca tgcccttatc cacctttgtc tgattggcaa
     8341 ataaatacaa taccttaggc accccattta ccacatggta ctttgctaca tcttccaatt
     8401 gatttcgcaa tccattttct ttatccaagt aaagactctc agtagccgcc gacaacattt
     8461 cataatcttt aacccttctt ctcccttcat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn tagatggtgg cggcatcctg atcgttaagc gtcatcttgc cgctgccgga
     8641 gaacaacccc atctccgatg cagggaatgc cagcgtgtat acaccgtcac tggtcacgga
     8701 ataatacgac gtacctgtga cataatagtc ctttccacct tccgtaaagt gattgtctcc
     8761 gccggcaatc acatgtgctc ccacttccgc tttggcagaa tttgtcactg taaatccggt
     8821 cagaggcttc accttcaatg tgcccttgcc gtatgcgatc tccatagcat ccgcatccgt
     8881 ggactccttg aagaactcta cccggatctc cgttgccgaa gacaggatgt tgtaatcggt
     8941 agccttgaaa tccacctgaa tcttattgct gcgatccagg atcatcttgt tggcagcatc
     9001 atacatcata tatacggagt caatggtgtt gccgttgtca tcccggaaaa atacggaagg
     9061 tgccttggtt ccggcattgt aagctgctac catggtgttc acaaacagct tcttctccgc
     9121 cattcctgta ggctccgtat gtcccgcacc ggaatacgtg acattgccca tgctgtaaat
     9181 ataatagtta tttaccacat ctttggggct gacattatac aggttcacac cattgtcatt
     9241 gccccgtccg ttgttcctct cagtctgtgt ctttctggtc ttggcattgt cggacaggtc
     9301 agacagacaa taccatacca caatatcatt tttgccgtct ccatccttat ccagctccat
     9361 gttcggctgc agccactgat aatgtgttgc cgccactgtc agagtctgct gttcttctgt
     9421 agtgatctga taaggataat tagtgatctg tccctgattt acctgcgtaa cgatcatatc
     9481 aggaccggca ccgtctccga attccttgta atctcccaac accgtgatct gcccctgaga
     9541 gttcgtcgtc ttatccatac cctgataaaa cagagaattg cgaatcggaa aatagaaact
     9601 cgtataagac gttcccttcc gcaggatcgc attgatccga ttctgcacat aacggactgt
     9661 cgtatatttg gtcgtgccct cgatatttcc gaccgtctga tcactgctcc ggtccgttct
     9721 cggcttccat gccgtatcgt aattgtactg cgtgtccaga atattgtaat aagcatcata
     9781 acgctttctc tgggcatcgg ataaactgga attgttcctc tggtcatcat aatattccag
     9841 caacgcacca taacgatcca gccctaccgc cgcacgcatg gtcttgttga actcgtagcc
     9901 ccagtaccag cctttaccgc taccggcctt actgttgata taagaactgc tgtcatgggt
     9961 aaacaggaca ctcttaccat cttcgatata atcacggatc gcttcagata ctgccatgtt
    10021 cttcaggctc tgatccttgc ttccgaactg atagttgtct ccgaatccca ggatcagcat
    10081 atcatactgc tggaaagtat tgtatgccgc attgtaataa gcctcagact tctgttcatc
    10141 ggtcttaagc tttttcaact gatcgtctga aatcaatccg ttgataaaag ttgaagtctt
    10201 cacggaatca atagatacat taaattcccg gataccgctt aacagtgact ttacatcact
    10261 gtcactttcc agattccaca gtgtcttgtc attagatgct tttctgtcgg acatcagctg
    10321 caatacctga atatccactc tggaacctct gtccactgcc gtataaccgg acactgcggt
    10381 tctcaccagt gcatcagaat tatcaaagaa gatcatcttc cagggcaata ctccctgata
    10441 atcatccggc agcactctga ctaccttata ggtatatccg gtgcgcagct cgtaatggcc
    10501 actgtcattc ttcgccactt ccttccattc ctgcccggat ctgacataca gattcagtcc
    10561 ggcaagctcc tctgtggagg aaagtgtctg cccgatcaca tcggaaccgg aaaagcgacc
    10621 gtcggagttc ttatcaatat acagcttgca atcatacgtc gtgctcacct gggaggcagc
    10681 cgcatcattg gacagagcaa aaatatactg cagatagaac ttaccgtccg tcatcttcag
    10741 gaacgcatcg ttgataccgt ccccgttgta atgcagctct gtaggaatat aatcttcccc
    10801 aagatttttc acccattcga tctgcagttt ggacagattc agatggttgc caaagtttct
    10861 ctgtctctct tctcttgctt cctttgccgc ctttgcggca gccagcgcct ctttgctgtc
    10921 atccgccgcc ttttctgctg ctccgggtac tagctgactc tccgcaaata cattctcctg
    10981 ccagtatttg taattgccgc tttcatccgt ggacaaggca aactgtatca actggtaaaa
    11041 ataggaactt tcgtccactg ttgccgtatt ggcactcacc ttaccgtcaa ttcccgtcag
    11101 aagtgcattc gcaaatacta ccggataccc ggcggaaata tactgctcga actccttttc
    11161 tctggtggag ttcatatcat ttcccgggcc acggtactga ttggaagtgt gactcaggga
    11221 atcatccttc agacggttct tgttatcatc cgattcatcc ttataatcat acagatcccc
    11281 gatatgcgta tagatcagcc cattcatgga actgtcattg taaacggtct gattgttttt
    11341 cacattcatg gttccggtgc gggcacccac atagatcata tcatatcgat cgttgagatc
    11401 ctctatcttg ccaatgaact ctgcggaagc catctgggta atggaaatcc ttccaccgta
    11461 actgctctgc cccagaaatc ctttcacgga atctgcagat aaagctccag ttccgaaatc
    11521 ataacagggc tccagatcca atatcgcaag gctgtccttt acctggcttc tgtgcagata
    11581 ccagttcata atatatctgg tgatggaagc tttgctgatc tcttcattca aagtgaccgc
    11641 cgtggcattt tcctgtgtga tcagaaaatt ctcgtagcgg atctctttca ggacatccgt
    11701 gtagatccat gctcttgtcg tatctgactt cagatccgtc agataatcct ctgccgcata
    11761 agctttggaa ctgtcgttgt tcaggtatac attttcgttc aggtattcct tcaaggtatc
    11821 caacgtcagg gtatccgcat ttgccgcctg cattgcagac gcataggatc tgatatttcc
    11881 ctctgccgcg cttttcagcg tatcgacact tacattttcc caataggaat ctccctgacc
    11941 tgcgattgcg gttgccacat tgtcatccga cacctgcagc agagtcaatg ccagtttcgt
    12001 caacacttca ttgctctggg tgctgttctt ggcataaaaa ttataatcca tgatcaccgg
    12061 cactctggct gcagcggttc tgatcccgct gtcgtccacg ccaaatgcca gctttgcgat
    12121 cacggttgcc gcagagacca tattggacgg ctctgccccg taatttccct gaccggaaaa
    12181 atagaacaga tccacatctg ccagatcaat tccacaataa gaatcctccg ctgtaatgga
    12241 agcatctgcc agatcagcca gttcctgcag ggtaagtgtt gtcacttcga tcttcagtgt
    12301 atcgatatcg tctccggtgg ctgtaccact ctcataacga ctggtaccgg acagattcag
    12361 gacatttttc ttgaaccatt ctttgttgta gaaaccgccg ttataggcaa tagtctccgt
    12421 agcatcctct ctgtcatccc ctacaaaatc atagctgttg gcggtgtcaa agcccgcttc
    12481 ccccttgtaa gtaacctgaa gggaacccgg tgtcaccata gtgtagctgc cggcctcatc
    12541 cttacgatag gtatcgatca gacggtaatc accgtttttg tcacttactg tcattgcaga
    12601 tacataatag tcgccgttat catttgctgt gaaatccaca agataggcac tggctatggt
    12661 ctcaaatgta tatgccttcg aaacatcatc ccattttgcc caaagctgtg cgctatccgg
    12721 ctgtgcatga tccaggatgt agagattctg ggtaatatct ccggcagaat aggctgctat
    12781 cgcatctcct ccggatgtcg tgcgtgcctg cacagcagta cctgcatcat ttccggatac
    12841 ccctctggac gtagtcttta atccatccgg cagtgccgca tcggtaccgt atacaatctg
    12901 tccaagatag gtataaactg cagtctcacc cgtacctatc ttctgataga ttgtcctgcc
    12961 cacataatct tttgcctgaa aactcgtttt atcaatagcg gccagatccg tctccgaaat
    13021 tgcatataag ccctgcgcat atcccctggt gctgtctgaa ccattgataa tcgtagtctc
    13081 cagtccgttg cttcctccgg tagatctatg ggaaaatacc ggggtcacgc ttttggcatc
    13141 ccggttactg tctccggcgg cctccaatgc actgtatcgg ttataacgtt ctcctgctgc
    13201 caccgtctcc ccattggcaa tctcacgata gaccgcatcg atctgttggt aattgtatct
    13261 gccggatccg tcctgtcgga atacaaagcg tcctcttacg tcctcggttc tggaagctcc
    13321 ggtgctttcg ctgtaggcag aaaaagtaac cggacctgtc gtcggaaact ttgcagcatc
    13381 cagactgtcc atagctgtct ggcgctctgt ggctgacggc atctcggaaa gctttctgcc
    13441 ctcagatacc ggctcctcgc cgcctgccag atagccgatc gtggcatttt ccttcgacgg
    13501 aacgatctcc agaatatgga aagtaccatc agaagaagct accgtatcct gtaccagttt
    13561 ttcaataccc ggaagggttc tttcctgcgc actgacctcc atagagccat agctctgacc
    13621 ggctccgacg accaaagcta tggtgaggat tcctgctata tacttttttg aattgcgttt
    13681 ccaattcatg gtcatttatt atccttccat tcccaccact ccggattctt ctctacttcc
    13741 agtaaaagat ttctcttatg atccggatat cgttcatcat aaatggtcat aattaaataa
    13801 tcattttctt catgtgcgcc aatatcaaac tgtacctgcg aaaaaggaag atgcatatta
    13861 tcaatttctg caaatttcgt gttggaatcc aacggcagtt ctccctggaa atatcctgcc
    13921 agttttgctg ctctatgtcc tttctctctt ccagactcaa aaccatacgc atgcagatca
    13981 taacgattcg tccaggtagg tctcagcacc gtaatcacat gctgatcatt ggcaaataag
    14041 atatccacta ccggcaccag tattaccgtt tcggccacta atgtggaatc atccaggctc
    14101 catgcctgaa gcttcagacg atattcggta tcccaggaaa tatagctaag tgctcttacc
    14161 tctgtagatt ctcccgtatt ttctgttgtc agatagcctt cctgatgaac attatcatat
    14221 gctccgtcat agacttcaaa accgacctga tatgattttt gtattccgtt ccataccttg
    14281 aaatcattat tcagctcact gggaatatct gcaaatctcc aatttacttc ccctttataa
    14341 tttcgaatct cgcaattaat ttttccatta cggtttaata caaactgact ggagtaaata
    14401 tacggttcct tcgcgtttac aaccgtcttc aggggaggga ttgtaatatc gatttctctg
    14461 gtaatcttaa tatctactga taaagctatc accgcacgta cacgtactac cgtatccgtc
    14521 atatttccac attccagttc tgcctggaac tgtcgaccat cagaatctgt caaaccggtt
    14581 attttgtaat tattggcata atcatctacc acactccact caatctgtgg ataaccattt
    14641 ctgtaatccg cccaggacgg cagacattca atcaatgcct tataaccgat cttttccgta
    14701 cttccttcgg taaaatcata tgtggtcaga ttcttggcaa tcagtgttgc atcatatttt
    14761 ccgtcaatgt tggtataggg taataccgat acctttgtgg atgcagacat tccgggatcc
    14821 gccacggaag tacaggttac ctgtagcaca agtgttccgg tcggctcata tctgtcagcc
    14881 gtcaaaagac cgctcccatt tatcgtggtt cctgccttaa gtccagttgc gtccactctt
    14941 gccacgctcc aggtcacccc ggcattttta gcctcttctg ttccttccat agtataagag
    15001 tactgcaggc tgtccccctg aatcacagaa gcatctttcg gttctatatc cagagaatcc
    15061 accatatgct tttgtaccgt tacggtagcc gttgccgcta tggtaggatc cgctacggcg
    15121 gtacaggtta ctgtcagaat attctctgct atggtctcat ccgctgccac actcagttta
    15181 ccgttaccgc tgatggtact cttacatgcg ctgccatcct gataacttac ggcccaggtc
    15241 acgccctgag caatggcttc ctcgtctccg gtgatctcgt aatcgaagac gaaagcctgt
    15301 ccctgtttca gatctgcgtg atccggaatg atacggatgc cgctgatcca ggaatagatg
    15361 ctggacggct cggagctcag atcatttctc agcttgatgg tctcggaaat atcataggac
    15421 ttgtcctgat aggtaaaggt catattcaga acgactttcc gcttatccag ttcactgagg
    15481 tccacgctga atctgctgac atgctctgcc atcagtgcgg gcctggctgt atcaaaatct
    15541 cctaccacaa ctttgccttc gccgtcctta gtatttgtta cctcgtaaag atacacctga
    15601 ttggcatcgg cataatccgc tccggcacca ccctgccagc ggatcatata gctgttgtcg
    15661 ttattgatca gacgcagttc actgacactg gcattggtta cttccatgcc gttaacgtct
    15721 tttgccacat tgccatcaac atcaaccgct tcctgatctg tcagattgga aaagctgatc
    15781 cccttctcca catcaatgat cagatcgatc atctggttgg cggtcagctg ggcttcttcc
    15841 tgcagagtca gttccttgtt tccccgcata taggaattgt tgccggcgat cacgaatgct
    15901 cccaccgcac ctgctactat ggtcatgatt gcaagagata cgatgatctc taccagagta
    15961 aatcctttct taccaatccc catggtcaac ctccttactt cgtcaggatc ttgccagtct
    16021 tgccgaccgt tgtgatttct atgtttctcg ttccgttgct tacgacgatt ttcagacaat
    16081 attccttcaa cacattgttt gccgtacagg tatccttctc aaaggccccg gatgcacggt
    16141 taaacaccag atgcagccca tttgaagatg aaacataagg taacaggctg tcggatcctt
    16201 tcagttccgt cagggaatat cccgtggaat ccccctcaaa atatactttg atatcaatag
    16261 gctcttttcc cagtaccctg ttggatacct ccacatctcc ctgccggatc tgtgccgtat
    16321 attccttggt cgtatcatta tagctgataa cgcactccac ctgattctgt tctttaccga
    16381 gggtcagcac tctggtccgc tccagtgttg tcacaatttc gtccgcacat ttccggactt
    16441 tcctgccggt cagggatgta atggatgtgg ccatcacacc caccagcacc gccatgatcg
    16501 caatgacaat gatcagctcc accagggaaa aaccactgtt gttattcttt ctgccagttt
    16561 tcataaacta tcaaatctcc cagatttata tcactgttct tataggactt accgttactg
    16621 ctcaggtaac cgtctccgcc atttaaaaaa tctaccacat gatattcctg tgtcttaccg
    16681 gcagcatcta cgatcgttct ggaataggtc agggcatgaa gcaccgcatc ccggtcgggt
    16741 tccacattga cattctgatc cagcgtgatc gttccgccgg acagaatcgt tccctggaag
    16801 ttctccgtca ctttgacatc tccgtcagca atgatcagcc ccttaaattc accgaatccg
    16861 gcttccggat aagtataatc accctttaca ataactgcct tatatccctg ctccgcaagg
    16921 cttcctgtgg tattgccgaa atattttgac tccaccttat ttccggtact gccgtttaat
    16981 acagcatgga aggtcgttat ctccccttca tttaccagat tggtataagc attggctgca
    17041 tccgtcttct ccgaaggaag cagctgctca tagacatcga tcatcttctt ggacaatgcc
    17101 tggaatttca actgatccga tgccaaaagt gcctgatatc ctgcattggc accatcactg
    17161 gtcagtgtgg catccaacag cttctctttg cttcccgcag ccgcactgct gtcatacatg
    17221 gccacattgc ctgccagatg catgatgtac gctccgttct gctgtccacg cacctgaatg
    17281 gctttggtat acagaccggt ataggcttcc atggtctgct ggttggcacc gtaataatcc
    17341 tgaaaatacc ggttagcatt ggctctcgcg gtatcagagg tgctgtcata gcttacataa
    17401 taatacacca gcgtgatctt gctgttgatc cgcttgaaaa tcttctggat attgttctga
    17461 gtcagtccgt actcggccag cgtatgacca ttcagcgtag cggacggaat attcaatgcc
    17521 acttccgtta catccgcctg gtccctgatc ttgtccttat actgctccaa cgtgatcggg
    17581 ttgctggcac cgtttaacac ggtcttgccg gaagcatcca cgccgataca ctgcgccggg
    17641 acaaggtaag ccagctggtc actcttcaca gcgatagact gtcccatcat cacatcctgt
    17701 actccggtgt tgctcacacc atcctcactg gtaattccat cattggcttt cgtcagagaa
    17761 atgtaagaat ttcccgccag cgtaagattc ttcaattcat tcagatagat ctccgtatcc
    17821 gtgccgttga ccagaatggc gctgctgtcc acgccctttc cgttgccgaa gccgatatac
    17881 tgtccggcat attctccgtc gatctgtgct ccggcggtaa atacattccg ctctccgttc
    17941 atcttgagat ctccggccac atatacgttg tttccctgaa agttcaacgt tccgttgctt
    18001 cccgtattgc ccggaatacc tccgttcatg gcaatggaac cggcccatac ttcaccgtac
    18061 tgatctgtgg taagtgtggc tccttctccc aaatataagt cggaagccat caccatacgc
    18121 ttgtcttcgt tttcattgcc ggtggtctct ttccattcca ccgtcacacc gttacccttg
    18181 ccagactctt cgctgcccac gataaaacgg ttcgcataaa agctgccgcc gatgacattg
    18241 gtgctgtgta gcgcaggatt ggaaggaagc gcctgaatat tctcctgtgc caccatggag
    18301 attcccgtaa tggaaggcag cgtcactgcc tgtgcaaaat ccatatccgg catcttcacc
    18361 cggatatcgg tctggatcac cgatacataa ccgtcggtat ccgtataagt caccttcagg
    18421 ccgcagaagg aaagtccgtc ggtgtacaac tgaaaacgtc cggtaggaat atccgtggtc
    18481 tccactctct ccttgttgcc cgtcaccgga tccgttgtca ttacatatac cttcagactg
    18541 ccgctgctgt ccagagccat ccggtacact acttccccgg agtcattcaa tgtctctagc
    18601 accgtccccc gctttatctc cgaagacagg ctgttgaaca gcttggtcag atcatagttg
    18661 ttgatattca tacttcctgt cactgcataa taatcctgca tatccttcag gaaataatat
    18721 ctcagtttgt tcagcttctc ctctgcggag gttgcctcat aatttttcag gaccagttca
    18781 taggcacttc cgacagaagc ggacacccgc tctcccatag ccgtccggat ctcatccagc
    18841 acagtctctg cagagtaaaa attgtccttc gccttgagat tgttcacctt catctgatag
    18901 ttcagcagtg acatatacat caaaacactg gctaaaatgc caataaaggc catggcaatg
    18961 atcacaacga ccattgctga gcccttattg tccgttcgtc tgtggaattg tcttaccctt
    19021 gaaaatattt tgggatttct tccgcatatc ttcatttttc tcgtcttcct tccggtatct
    19081 tctctgtcct gtgcctgacg gatcactcga tcacggttcc cgtcatggta agcacagggg
    19141 actgtacacc gatcgtatcc ctgctgtaca cctgtacccg catgtcatag atacgattgc
    19201 gtcgctccgt cggtgtcaga ccgccgtccg cacccataat atccttcgcc acgtctccgg
    19261 cagcatttcc tgccgcaccg gacacgctga gatcattgaa aatgtattcc gtattgctgc
    19321 ggtctatcag attcgtccgc agtctgcagg cggaatcaat gctggctccg gcagaggaca
    19381 tccagcccgg ccaggtctcc cgcagttcaa atgccgcctt gtaattgttg ttccaggagg
    19441 aatacgggtt aaaaccggtg ctttcgtccg tgaagctgtc tattttctcc gctgacggag
    19501 tctgctccac caggaacaga tttgtctgta cattcttcgg gttatttacg atcactttgt
    19561 ctaccgcaga agtcaccgca ctctgggatg cctccactct gggctgcagg cacacaatga
    19621 tattggccag ctgggaaatg atctcctctg catctaccgg attcccgctc tccttctcct
    19681 tcttaagctc cgctgctttg gctcttaccg cctcttcgtt ggaaaagatc acataatccg
    19741 taccgttctg ggggtagtct ccgctgatgc ctgtaaggat ggcggaattg caatgataga
    19801 ctgtggaggc cgtcaccaga tacacatctg tctctacgtt ctctccatct cccagatcca
    19861 gcaaaatgcg gctgctgtct atggatattt cgatggtctt ggtagtattc ctggcaatct
    19921 ccataacgtc tctgccgccg gaatacgctt caaaataacc gtatgcctcc tgtgcggcat
    19981 cttccgattc ggaatatacc gcattcagat tgcctgccag gttctggata tttacaatat
    20041 ccttctcatt cagtgccgtg taaccgttag gatccagtgt tactaccaca tcatagatgc
    20101 cggaagtcgt cttctcatcc cggaaggtat attgccagat accatcttct gctgcgccgt
    20161 ctccgtccac atccagagag acatctctct gcacattgtt agtgaaatca gtgggcgtga
    20221 tcgtctcata ggtgtctgcc atctcctcca gtgtatgtgc ctcgaactgc tccatgacat
    20281 cctctgcgat ggccgtggca tgcatggtgt tgcgggactt ggcattggtt ctggcagaag
    20341 tcacaaagct gtgcaaaagc ggcacaacga tgatcgccag gatcacgatg gcaatcagca
    20401 gttcaatcag agaaaagcct tcgttattca gttgtcttct ctccgtgtac tccttcatgt
    20461 gaggcatcct ccctgctcat gactaatacc gttttctccg cactccgtgc tcccgaaacc
    20521 ggacaaccat tcgcactccg gactatcgtc ctccctgctc atggttgttt gatttgctaa
    20581 tcatagcagt atcctgcctg caagcagtcg tatactgcta tgattgcaaa tctattattc
    20641 cacgtttccg aaaatagtgt ctacgcccat ctgcatatcc gggatgtaag gtgaactgta
    20701 taccttatcg gttcccagca gataataggt gttcatggtc atgttgccgg tgagcattcc
    20761 gtcggtctcg ttgtagctga tgctgacgcc ctggatgctc tcacgttcct gctgattgta
    20821 gatgtatctt accagtccct tgaaatcgtc ataggtgcag gaaatgctga tgctcagggg
    20881 tacctcatac atatgggtat ctgcataagc agtaccgtcc gtagataccg gacttgctgc
    20941 cgtcgttgcg gcagctgctg tggtatctgc cgcagcatcg gctgctgcta catccgtggc
    21001 agctgttgca tccgcagtcg tcgttgccgc atccgctgca ggagtataca ccacctctgc
    21061 ttcggaaccg aatgcaatat ttgccacctc ggtacgagta taatcttcca atgtctttac
    21121 catcataatg ctgtcttctg gtaagatatc tgccgggaag cggttctcaa aattcgtgat
    21181 ataggactgc atcttcccgg tagcctcaat gtagtccgcc ttacgctgat ctaatatctc
    21241 cagcgttgcc acctgggatg ataacgttac attctgtgcc tcaagggcat ctgatttttc
    21301 attaaaactg gaatatacca gaaaataagt acacaccgca ataagaacac cgatcactgc
    21361 gatcaaaagc tgaatttcac tctttttaac cttcatcttc cgctgcctcc tactgtgctg
    21421 tctgggtgtc ggcctgtgcc tcaccgctct cttcggtctc atccgtcagc tccttatagc
    21481 ttccggtaat ggaaaaagtc actatggacg tgccggtctc atccttgctg tccgtaatat
    21541 tgctgatctc tacatcatca atactctgca tgttgcggat attcagaatc gttctggctg
    21601 ccgccgcttt cccttccacc gtcactgaca tgctgatgcc ggtctggtcg gagctgaagc
    21661 tgttggtata gaagctgtcc ggcaggatct gttccagttc attgatgaac tccaccagat
    21721 tctcattggg agtctgggta taagcatata aatatgtgta tttatcatac tgggcttctg
    21781 ccgccagata atcgttgtat actgcctgtg ccggctgcag ctcctgtacc cggttctgca
    21841 gatacacatt ctgcgccaca tttcccagat atctggtcac agaggtggct gccatggcga
    21901 tggacaggat cactcccaac accagtaccg ccacactgac gaaggtataa ttcgtgccgc
    21961 tgaccacggt cagtcccttg gacttctggg tgctcttgtc gatcagtccc acaggtgcga
    22021 gcaccgcacc cagacaggaa atataggctg cgggtctggt ggaacggatg gccttgctca
    22081 tgccgatctt gtcatccatc tcggacaggg tatgtacctt gcgctccaga cagttggtga
    22141 acagcttgct catgccgctg aagctgccgc ccagaccggt aacataggac ttttcaatgg
    22201 gattttcact gttcctggaa tcatagaagt caatgacacg gctcacaccc acgatcagag
    22261 gctccaggga agcggtgatc ttctgtcttg ctgctgtgac ttccacatta ccggcatcct
    22321 catcttccca gcgtccggca ttttcttccc agactttgtc tcccggatgg agtactctgt
    22381 tcagacatgt tttcttctgg aaacgttcta ccgcactcat gggatcgttc acggcatagg
    22441 ctccggaagc gatcatagta tcaatagcat cctgcacacc gtaagccacc gttctctgca
    22501 gcaacacctt acctgcggaa agcacggtca caatggtgct gttctcatcg attttgatga
    22561 ccatggtaac tttttcggac ttctcatcgc ggagaatctg gtataagctg ttgctgctgt
    22621 agtcgaaaca ctcgatctcc cagccgcaca tctgtgccaa ctgataatag ctgtttaaaa
    22681 gagccttggg tacggccaga gccatgacac gaagcttgcc attctctgcc agaccgcctg
    22741 ccagataatg tccgatctca tattgggtca gatccaccgg gaaatagtca ctggcattgg
    22801 tcttcaccag tgcctcgatc ttgttcgcct tgacattggg aatctgtacc tcacggcttg
    22861 ccactctggt agaagaaatg gtaaacagca ctttcttcgt acggatgcca ttcgttccca
    22921 gtgcattcac cagatggtct gcgtactcct gtgtcggctg cagcatacca tccaccacga
    22981 tgccctccgg cgtctccact gtcagtacct tatagacctt cggctttttc accttatagt
    23041 ccatctcgca gatcttcgta aaagagtttc cgatctcaat actcaatacg cgattgttca
    23101 ttgccatatt tctacccttc ctcttcccgg tatttatcgt atttcaaaag ccttccgcca
    23161 tcgcggaatc tttgaagaac tttctgaaat catgttcgaa atgcggatat cttttataag
    23221 taaaaatgca gataccactg taagaattca tt
//
LOCUS       DAWEJW010000038        22774 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_914_length_22774_cov_50.705445, whole genome shotgun sequence.
ACCESSION   DAWEJW010000038 DAWEJW010000000
VERSION     DAWEJW010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 22774)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22774)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22774
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_914_length_22774_cov_50.705445"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         6616 a         4359 c         6085 g         5714 t
ORIGIN      
        1 tactatgcac ggccttacag gtactgaata tcgcagattt tatctcgaca atgtttttgt
       61 cccacatggt gatcgcagga accttcctga cggtatttat caccatttgc agggatctta
      121 ttcagggaac agcgaagcat tacaagctgc cgctgatcgg gctggtggct gccatgatcg
      181 cagtcatgct ggaggtgaca gcagtgtacc gtgttgtgtc tctgtccggg atctttatcg
      241 caaccggact ggtggtcctg ctggtcgtta ctctgatcca gacgatggac cgcattcgtg
      301 agctggagct ggacaggcag agagaggcaa gggaaagtct ggattatctg acaggtcttc
      361 ccatgcgtca taagggtgag gcactgatcc ttgagaaaat gcagaagcag ggcggctgtc
      421 tgggatttgt ggatatggat aatctgaaga agatcaatga tgtctatgga cataaggcgg
      481 gggatcatgc cctcagactg gtgggagcga cgcttacaga gtgcatgaag aacgcagtag
      541 tctgccggtt gggaggagat gaattcctgt tctatctgcc ggaggtatcg gaagcagaga
      601 taaacatgcg ggtgagaaag ctgtttgaca gctttcgtgc ggcaaaaaat gctgcggcgg
      661 agacttcagc ggcatccctg tcctgcggtc tgtgcatgtg cagacaggga gacaactttg
      721 aagaatatta tataaaagcg gacaaagcat tgtattatgt caaacagaac ggcaagaata
      781 attaccggtt ctatgaggag ctggaggaac agcagctcac gaagcgatca gtaaataatg
      841 aggggatact tataaaagat tcttagggta gtaggagcag atatggcata tcaatatgag
      901 aaggtaaatc acgaactgct ggttccacag atcgaagacc agctgatgat gtatatgaag
      961 caggagaaca tagccatcgg agaaaagctg cccagtgagt acaagctggc agaattgttc
     1021 ggcgtggggc gcagtacgat ccgtgaggct gtgaagagcc tggtgactaa gggtgtcctg
     1081 gaggtgaagc gaggctccgg cacctatgtc agaagcacag agatggtggc agaggatccc
     1141 ctggggctgg cgcagttcga ggataagtat cgtctggcaa tggaactgtt cgaggtcagg
     1201 attctgctgg agccggaggt cgccgctgtg gcaagcagga atgccacgga agaggagaag
     1261 cagcatatca aagagctgtg tgatgagacc gaacggatct atctggaggg gaagaaccac
     1321 atccccaagg atatggaatt tcatgaggct atagcaaaat gcagcggcaa ccgcgtgttt
     1381 gaggtacttg tccccattat atacactgcg gtgacgactt ttgccaatct gaccaacaga
     1441 cagctcatga aggagaccat agagacccac agagccatca ccgatgccat cctccgggga
     1501 gacagcatgg gggcaaagtg tgctatgatc atgcatctga cctacaatcg tcagaaactc
     1561 ctggaactga tcgaacaaag agaaaagtca tcagacaagt taatgccata aaatgacaag
     1621 tattgataaa attatacgaa aaaccagcct gggtaacacc atcaggctgg tttttttatg
     1681 cgatatgtgg aaaaaagatg tagatttgaa acaacttgaa gaaggcacca aagacattta
     1741 tacttaagat acaaagaaag atgtcagaca tatagagaga acaaacggtt acagaaaaat
     1801 aggaggtatg aaaatgacag agtttgttgc ttcgccggaa agattgatta ttggagcact
     1861 gttaggcttc gcagtattac tttttttaat cattaagctt aagatccagc cgttcatcgc
     1921 gatcatgtct tccgcactga tcataggcct ggtgtccgga atgccctttt ccatgatcac
     1981 ggacactctg gctgagggtg tcggcaatac cctgcagacc attgccatac tgatcggtct
     2041 gggctccatg ttcggagcga tcctgcaggt atccggtggc gtggaggtca ttgcggatac
     2101 gctgctcaag gtattcggag agaagaaagc gccctgggca ttaggcatta cgggactgat
     2161 cgtgggaatg ccggtattct tcgaatccgg tctgctgatc ctgatccctg tggcattcgg
     2221 aattgcccgc aagacgaaca aatccgtatt taattattcc atccccctgc tggcaggctt
     2281 ggcgatcgga catgcttttg tgcctcccac accgggaccg gtgctggtag cggaaatgct
     2341 gggagtagag ctcagctatg tcatcatact gggcatcgtc atcggtattc cggcgatgat
     2401 cctggcagga ccggtgttcg ccggatttat cggtaaaaaa atccattgca cggtgccctc
     2461 ttcctatctg gaaaatgaaa aagaagcaga gcagaaaaat accggtaaaa aaccgaattt
     2521 ctggctgatc gtatgtatca tcctgcttcc gctgtttttg atcatcctga atacattgtc
     2581 ctccgtgatc ccgttcatga agccggcaca gccggttctg gaattcctgg gtactccggt
     2641 catcgcactt ttactgtcaa cgatctgcgc catgctgttt ttgggactgc ccggtggctt
     2701 cagcatcaaa gagatcacga acattatgac gaattccctg cactcctgcg gtaatatcat
     2761 cctgctgatc gccggcggcg gaatgctgag atttatcctg cagaactccg ggatcggaga
     2821 attgttcggt aatttcgtag gcagcctgcc tctgccgatg gtactggtag cattcctggt
     2881 ggcagcagtg gtcagaatct ctgtgggctc ggcaacagtg tccatgacca tggcagcggg
     2941 gatcattgct tccatgccca tggtatccgg gttctcacag ttacagcttg ccacgatcac
     3001 cattgccatt gcggcaggcg gcacagcctt cagtcatgtg aacgattccg gcttctggct
     3061 gtccaaatct ttgtttgagg tggatgagaa gaccaatctc aagacctgga ctgccatgga
     3121 gaccattgtg ggtatcgtgg gagtgatcgg agctactatc gtatggtttg tagtgtctta
     3181 aagaaaagga gagatgatta tgcttagaag tgaagaagtg agaaaactgg caccggagat
     3241 ggatccgctg cgtatgggaa tgggctggaa ggtaagtgat ctgtccaagc cgcaggtcat
     3301 ggtggaaagc tcctacggag acagccatcc cggctccgcc catctgaata tattcgtgga
     3361 agaagcggta aaagctgtgg atgaaaatgg cggtaaggca gccagatatt ttgctactga
     3421 tatgtgtgac ggtatcgccc agggccacga tgggatcaat tactccctgg cacaccgcga
     3481 cgccattacg aatctggtgg aggcacaggc caatgccaca gtattcgatg gaggcgtatt
     3541 catagccagc tgtgataaat ccatgccggc catgctgatg agtatcggac gcctgaagga
     3601 tatgagtgcg atcgttgtga ccggaggagt catggaggca catgagctgc cgaagaaata
     3661 tgtggtggac gatcctgcct gcaggatcaa tgagctcctt actctggaac agattggtaa
     3721 attcgatgcc tgggagaaga cgggagtcat ccccaatgaa cagttggatt attataagca
     3781 gcatgcctgc cccagctgcg gagcgtgctc ctttatgggc acggcgtcca ccatgcagat
     3841 catggcggag gctctgggac tgatgcttcc gggcaccgca ctgatgccct ccacggcacc
     3901 ggaattacgt caggcagcct acgatgcggg aaaacagctg atggagctga tcgcaaaagg
     3961 gatcaaagcc tctgatatcg taacgaagga aagctttgaa aatgcgatca tggtgcatgc
     4021 agccatttcc ggctctacca atgccaccat gcacatcccc gccattgccc atgaattcgg
     4081 ttttgagatt gatgcagata cctttgaccg cctgcacagg ggcgctcatt acctgttgaa
     4141 tatccgtcct tccggagact ggcccgcaca gtatttttac tatgccggcg gtgttccccg
     4201 cgtcatggaa gagatcaaga gcatgctgca tctggatgtc atgacggtga cgggcaggac
     4261 attgggtgag aacctggagg agctgaaaaa gaacggcttc tatgagcatt gtgatgaact
     4321 gctccgcaaa aagtcgaaat tattcggaag aactatcgat agagaggata ttatccactc
     4381 cttcgagaac gccaaaggta cggagggcag tatcgctatc ctgaagggca acctggcacc
     4441 ggaggggtgt gtgatcaagc atacagcatg cccgaaaaat atgttcaagg cagtgctgac
     4501 ggcaaagccc tatgacagtg aagaagcatg catcagtgca gtgctgcacg gagaagtgaa
     4561 gccgggagat gcaatattta tccgctatga gggccccaga ggcagcggaa tgcccgagat
     4621 gttctatacc ggagaggcta tctgtgcaga tccgaagctg gcctccagcg tggcactgat
     4681 cacagacgga agattctccg gagcctccag aggaccggtc atcggacatg tatcgccgga
     4741 ggcggcagca ggcggtccca ttgcattcgt ggaagaagga gatctgatcg aactggatgt
     4801 ggagaacaga aagatcgcca tcgtgggtat ccggggcgag aggaagactc cggaggaaat
     4861 agacgcagtc ctggcagaac ggaaagccgg ctggaagggc tttgagagta aatatcagca
     4921 tggactgtta aagctgtacg cacagcatgc cgtaagctcc atgaagggtg cttatatgga
     4981 ataaacatta aataatgtca gaactgtcag gcacatcagc taatctgatg tgcccttaaa
     5041 ttatatactt ttacattgtg ggaaaaacta aggatgaaag aataatattg ttccgtttag
     5101 cttacgtctg agagcagagt tcataatgga atcataccat ggatatttgt caaatagtaa
     5161 caatattagg gaagcatatt aggctatttt actgtattga caagtgcggt gtgaaactat
     5221 atcatttaaa tagtttaacg tttacgtaaa cgataaacta aaaaagatac agttgcctta
     5281 tggattcaaa tatcaagagg gatggttgct tctgttatga ccgcatattt cagtaggagg
     5341 taaaaacatg cagggagaaa tagaatatca tacgaaagaa cgggtaacga ttaaagatat
     5401 ttctgctaaa ttgggtattt catctacaac tgtgcatcgt gcattggcag gaaaggaagg
     5461 catgacggat gaactgcggg ataagatcct tcaaacagcc cgggagatgg ggtatgagat
     5521 caattatgca gcatcctgca ttaaacgtaa gttacaaaaa attgcagtga tattgccatt
     5581 agaagaggac agatattttg ataatatctg gaaaggcatc cgaaaacagg cagaagaagc
     5641 gagacctctg aatgtagatg ttgaggtttt tgcctgtaag gatgaatggg atgaacggga
     5701 tattttgaag acgattgcag ataaaggagc tggtgagtat gcaggagtac tggcattttc
     5761 ttattccagc agttattcca gaaccactga aataatgatg gaactgcagc ggttaattac
     5821 gatgaaaatc cctaccttta tgattgatga tgatatggtg gaacctgccg gaatttattg
     5881 tattcctgcc tatcaaaaag aaattggaga gctggcagcc gagttgacgg ctcttatgac
     5941 cccggaagaa ggaactgtat tggtcagcag aggacgtgag gattctacga ttcacaggga
     6001 gaaactgcag gcttttcaag actatttaag aaaacataaa ccggggttga aaatagtctt
     6061 gatagacggt tattccacga aagaagaact ggatgggccg gttcgtgaaa acattaaaaa
     6121 ggcacttcag gaacatacag atgtcaagct ttattatgcc cagacaagcg gtgatacccg
     6181 tgtggctgca gaggtgttcc gggaaaataa agaaaaatgt aactttgtca gaatcggaac
     6241 agatttaaat tgctataccg cagaaagcat tcgcaagggg gaattgacgc tggtgatcga
     6301 tcagggggga tacagaaagg gctacatggg attaggcgcc ctagtggata cagtagtaaa
     6361 acatatcaag ccggaacaaa aattaaacat tactttagat gtggtctgcc aaagtaatct
     6421 gattttttat gaacatacaa atatgtaata ttagaaaaat ggtgattata aacagtacag
     6481 cattgctgtg catttataat aaaatgtttt aagtaaggta acacgaaagg agaaaagcaa
     6541 aatgaaaaag gaactgaaaa gactaggaac attgatgtta gctgcggcta tgtcagtttc
     6601 aggtcttaca gcatgtggtg ggaaaggtaa tgaaacaggt gcggcaacag aagtgactgc
     6661 tacagggggt gctgctacac aaagcacagc ggcagaagca gcttcttatc cgcttgatac
     6721 agacgtgacg gttacttact ggtcagagct gaatggtaac gtggcagcaa acttcaataa
     6781 tatgggagat actgaatttg gtaagaaatt acaagaagaa agtggtgtga aaattgaatt
     6841 ccagcatcct gcagtaggac agacggcaga ggcatttaat ctgttgttgg gaaaaacaga
     6901 tctgccggat attattgaat ataactggct gggttatccc ggtggaccgg aaaaggccat
     6961 caaggatgcg gttattattc cgttaaatga tgttattgat aagtattgtc caaaccttaa
     7021 ggcatatctg gaagctaatc ctgagattga taaaatggtt aaaacagatg atggtaccta
     7081 ttattgcttt ccgtttatcc gtggaggaga cagattactg accaacatgg gacttatgct
     7141 tagaggtgac tggttagagg aactggaatt ggaggttcct actacaatgg atgagtggga
     7201 aacagtcctg acagctttta aagagaaaaa gggtgcaaca gctccctttg cttatcagtg
     7261 gtctttagcc ggcttaacca ataacaatcc atttgcatat gcttatggag cttccaggga
     7321 attctatctg aatgcagagg gaaaagtgac ctatggagct gtacaggatg gctataagga
     7381 atatctgatg acaatgaata aatggatgaa agaaggattg gtcgatctcg accttctggc
     7441 attaacaggt gatcaggttg cagctaaaat tacgaatggt accgcaggtg cttcttttgc
     7501 atattgtggt tctggtttag gcaactggac caaagccgga caggcaacga accaggatta
     7561 tgtgctggtt ccggcaccct atccgacatt gaagcagggt gaaaaaccgg agatgggtca
     7621 gagagacaac aattatatcg gtgtgggatc tgctgcgatt actacatcct gtaaggaggt
     7681 agaggttgtt gccagattat tagactatgc ttatagtgaa gcaggggcaa cattatacaa
     7741 ttttggtaca gaaggtgaaa gctataccgt agtagatgga caaccggtat atacagattt
     7801 agtgttaaac aatcctgatg gtctctcagc aacacaggcc attgcgggct atgcacgtgc
     7861 atgctacaac ggaccgtttg tacaggctga ggagtatgcg gaacagtatt atacaacaca
     7921 ggagcaaaag gatgctattg cgatctggtc tgataccaat atgggcgaat atgtgattcc
     7981 gcctgttaca gcgacaccgg atgaagccaa agaaattgca tcttatatgg cagaaataac
     8041 tacatataga gatcaggaaa ccattaaatt tatctatggt gaccgtagct tcgatgagtg
     8101 ggatgattat gtggcagcca ttgaaaagat ggggctcgca agagttcttg agcttaaaga
     8161 agcatctttg gaaagataca agaatagata atttgtgaac acgtaccgga agcttcacta
     8221 tgagtttgcg gcattatctc gaaatgggag gtatctattt catgaaaaaa aatattccaa
     8281 atgcagagag acttggagcc agaatcagac gtgattggat tcgtaaccgc tcattatata
     8341 tcatggtgat tccggtactt cttttttaca ttttatttca ctataagccc atgtatggca
     8401 caattatggc gtttatggat tatagcccga gacttggctt tgctggcagt gaatgggttg
     8461 gttttgataa ctttatcagg ttctttcgca gtccgtattg ctggcaggtg attagaaaca
     8521 ctttgatcct tagcgtttat ggtatcgtta tttgtttccc ggcacctatt attcttgcac
     8581 ttctgataaa tgaagtaaag agccagggat ataagaaagt ggtacagacc gttacgtatc
     8641 tgccgcattt tatttccctg gtggtagtta ccggtattat caaggatttt acaatgagta
     8701 ccggtcttat tactgacata gtggtatttt ttggtggaga gagacaatct ttaatccaga
     8761 atcctaacct gtatcgcacc atttatatcg tatctgatat ctggcagggg atcggatggg
     8821 gctctatcat atatttgtct gcattggcag gagtgtcaga agacctttat gaggcggcag
     8881 caattgatgg cgcaggacgt tggaaaaagc tggtgaatgt gaccattcct gcgatcatgc
     8941 ccacgattgt aacgatgctt attatgagac tgggacaagt actgggttct aattatgaaa
     9001 agacaattct tttatataat gaagcaacct atgagactgc ggatgttatt tcctcctatg
     9061 tgtacagagt gggtctgtta gaccgtgatt ggggatactc agcagcgatt ggtctattta
     9121 actcgttgat caatgtgata ttattactga taacgaataa gatcagtaag aaactgacag
     9181 aaacaagctt gtggtaagga ggacaaaatg gcggaaattc aaagaagaca aataaaagag
     9241 tcatttggct ccagagcatt tgatgtagtc aatattatta ttcttgcttt attatcgttg
     9301 gtttgtttat atccggtctg gtatgtgtta gtggcatctg tcagtgatgg taatttatta
     9361 atgcagcata caggcggact gttaaagcct cttggattca gtcttggagc atacaaaaag
     9421 gtattccaga atcctatgat tatgcaggga tatctgaata cacttaaaat tcttgcgatg
     9481 agcatggtgg tacagctgac catgactgct attggtgcat actttttttc cagatccaat
     9541 gttatgttta agagaccatt aatgatcttg atcaccataa caatgtttat caatggtgga
     9601 atgatcccct tttatcagac attgcaggat ctgcggttac tggaaacgca tttgggatta
     9661 gtgatccctt ttgcgataag tacctataat atgataatat tgagaacctc gtttgaaagt
     9721 attccggaat ccttaagtga agctgctaga attgatggcg ccggtcattt caccatttta
     9781 tttaaaataa tactcccctt atcaaaatct attctggcag tcatggcttt atactatggt
     9841 gtaggaacat ggaatggctg gttctggggt tctaccattc tgacggacag aaccatgtat
     9901 ccgttacaag ttgtgttaag agagatcatt atgtcaaacg ataccacatc catgacgggt
     9961 ggcatatcgg caggtgatgt agaagcaatc ggtgcgacaa tccgttatgc tacgattatt
    10021 gttgcgaccg tgcctatttt atgcgtatat ccattcctac agaaatactt tacaaagggt
    10081 gtaatggtag gagcagttaa ggagtaatat aagaatatga tggaaactaa attgcagcag
    10141 atcagagaac atcatgcagc atttagaaaa catattgaag agacaggtaa ttccttgcgt
    10201 tataaggaat tacctgtttt gacagaagag atgtttttgc tgtatgaaaa aaccggaaat
    10261 cgtctgatct atgaaaatga atattttaaa agaagagaat ttttagttgt ttttggttta
    10321 ttggtatatt ggtataaacg accagaagat attgaaaaat tagaagaagt cttgcttgaa
    10381 gtatgcggag aggaaacttg ggcgcttccg gcacatgttg atcttaaaaa aatgcagtgg
    10441 cagagagagg tggatctgtt tgcagctgag acgggacaga ctctggccaa tattattcat
    10501 ttaaacaaga attatttaaa gcctgaggtt gtacagaggg taaaggatat ggtcaaatac
    10561 cgtctgctgg attcttatat ggaagtaccg gcggggggat ggagatggga acatttttat
    10621 aataactggg ttgcggtctg tgcttcctgt gtgggaagtg ctgcactgtt tttgttaaat
    10681 gataaggcag aacagaaaaa agcaatcatt gagcgtgtat gtaatacgct gccggcctat
    10741 attgagggga tgcatgatga tggaacctgc ccggagggta tgagttattt cacatatggc
    10801 atgacctatt ttgtcggttt tgcaagacaa ttaaaagaag ataccgatgg tggaatcgat
    10861 ctgatggatt ctgagaaagt cagaaatatt gccagattcc aacataaatg ttatctgccg
    10921 gggggcaata ctgtgagctt ttccgatggg caaagcgagg atcattaccg gctggggctc
    10981 acctgttatc tggcacgtaa catagaaggt gtagtaattc cggatataag tgctgcaatg
    11041 gaatttgaga tggatcattg ttatcgattc atgggaaatc tgcaggatga tatctgggta
    11101 caggaatatc tggaagcaga ggatgagaaa agagaagaag gtgaggaatg gtttaccttg
    11161 cttccacagg cacaatgggc agtatggaaa aaaggcagaa ccggtattgc attcaaaggt
    11221 ggaaataacg atgaaccgca caatcataat gatataggaa gctttttact gacagtagaa
    11281 ggagaagtat ttctgacaga cctggggtgt ggggaatata caaaagagta ttttcagccg
    11341 gataccagat atgatatttt gtgtaacaga tcttatggac ataacgttcc gatcatcaat
    11401 ggctgtgagc aatctgcagg aagcggattt cacagtgaat acttcagaag tgattgtgaa
    11461 ggatgtgtgg aattatcctt tgcaggtgcg tatgaacccg gtgcagactg gaagctgaag
    11521 cgtatcgtgg aaagctatgc aacagacagc attgtaaaga ttacagactg tatttgtaat
    11581 gcggataaag tgttatttga ggaacggctt gtgacacaga tagaaccgct aatacaggag
    11641 agtcagatcc tgctgagggg acaaaaaggg gatcttgcaa tcaaaatcac agaagaatgt
    11701 gcatcggtgc agatgaaaaa agagattttc agaaatcata gaggaaaaga tgaagatgtc
    11761 tggctgctga catttcctgt ctctgtcaga gaaggaaagg gaaaatgtca gatttgttgt
    11821 gaatatataa aaaggaaaga tggagaggaa attctatgca gacaatagaa aagcttttaa
    11881 cgtacccgga ggctacagat acggaagtag aggaactgtt acagaaagca tccgacattg
    11941 tgattggaaa tctggatgtt tttacagaga agttccagca ttccaatagt gaaaatggtt
    12001 tctatgaagc ggtaccgaat atcagctgga caactggctt ctggacagga gaaatctggc
    12061 ttgcttatga aaaaacaaga gatgaaagac tgaaagaggc cggtgaagtc caggtacgga
    12121 gttttttaga cagaattatt gataaagttg aggttgctca tcatgatatg ggctttttgt
    12181 attcaccctc ctgcgtggca gcttacaaat taacaggcaa tgaagatgct aaaagagctg
    12241 cagtgctggc agcagaaaat ctgattaccc ggtttcagga gaagggacag tttattcagg
    12301 catggggaga attgggagca gaggacaatt atcgtctgat tattgactgt ctgatcaatg
    12361 ttccgctgtt atactgggcc actgaggtta ccggtgatgc taagttcaga gatattggag
    12421 aacgtcatgt aaagacggcc atgaaatatg ttatcagacc ggatcattcc acgtatcata
    12481 cctttttctt tgatcctaaa acaggtcttc ccaaaaaagg tgttacacat cagggatacc
    12541 gggatggctc tgcatgggga cgtgggcagg catggggaat ctatggtgct gcacttagct
    12601 atgcgggctt gaagcaaccg gaatatcttg agatttttga acagcttaca gatttcttcc
    12661 tgacacatct tccccaaaat cttgttccat actgggattt tgattttgac gatggaagtg
    12721 atgaaccccg tgactcatcc tgtgctgctg tagtagcatg cggtatgctg gaaatggcaa
    12781 aatatctgtc ggaagaaaaa gcacaatatt acagaggcat ggcgaaacgt ctgatcaagg
    12841 cactttcgga tcagtgtctg gtaaggaata gagaagaatc caatggtatt ctgttacatg
    12901 gcacctactg taagagttca ccatacaata catgtcccaa ctggggagta gacgagtgcg
    12961 tcagctgggg agattattat tacatggaag ctttgaccag attacataaa gaatggaatt
    13021 cttactggct gtagcatttt atttattttt gtaactattc agagccatgc aaaactgtat
    13081 tctgcggatg cttgcattca ggcagataag cagttttata tggcgaagta acctcatgag
    13141 caaaaaggaa gcctcgtaga ggcgattttg taaatgggtt ctgaatagtt acttattttt
    13201 tattaaagtt tacaaaattt tacgataatg ttcaaaaata tacaatttat caaaagtgat
    13261 gtagacaaac aataaaaaag tgggtaatat gtatatataa taagctcgac aggcaaaaag
    13321 tatgtacaat atgctacata attaaagcaa gaaatataaa atagagagga atgttgtcga
    13381 tgaaacagtt cagctatgtt attaaggatg aaatcggtct gcatgcacgt cctgcgggac
    13441 tgctcgccaa ggaggcaaaa aagtatcaga gtacagtgac actttcctgt aaggggaaat
    13501 cagctgcagc aagcaagctg atggcgatca tgggaatggg cgtgaagcac ggagacaacg
    13561 tggaagttac ggtagaagga gcggatgaag ataccgccgc agcagagctt cagaagtttt
    13621 tccgcgagaa cctctaggag aagacctgcg caacggagtt tggaaggtag aaaatggaaa
    13681 aatatattgg aaaatccgta tataagggaa cagcgatcgg acctgtcagt gttctgaaga
    13741 aaaaggacag tctcgttaag agaatccata tcgacaacac agcggaagaa ctgaaacggc
    13801 tggatgcggc taaggaacgt acgatgacgc agctggggca gttatacgag aaggcgctgc
    13861 aggaggtcgg agaagtcaat gcagctattt tcgaggtgca tcagatgatg ctcgaggatg
    13921 acgattatca ggatgccatt cacaacatga ttcagaacga agaagtgaat gcagagtatg
    13981 cggtggcagt gaccggtgat aattttgcag agatgttcgc caacatggat gatgaatata
    14041 tgcaggcccg ctctgcggat gtcagggata tctccaaccg gctcgtccgc aatctgtccg
    14101 gggaggagca gattgactgg agtaccatgg agccgtcgat cattgtggca gatgatctga
    14161 cacccagtga gacggtgcag atggataaga gcaagatcct ggcctttgtc acagtacatg
    14221 gctcaacgaa ttcccatact gcgatcctgg cacgtatgat gaatattccg gcactgatag
    14281 gggtaccatt ggaactggag aaaatccata acggaacccg tgctattgtg gacggcaggg
    14341 aagcggtatt ttacctggat ccggaggaag agcagctccg gcaggcagaa gcggcacagc
    14401 agacagagca gagacagaga agcctgctgg cagattacaa gggacgggaa tccgtgacaa
    14461 agagcggacg caaggtcaat gtatatgcca atatcggcag cgtatcggat gtggcatatg
    14521 tactggagaa tgatgcggag ggtatcggcc tgttccgcag tgaattctta tatctcggaa
    14581 gggactctct tccggatgag acagaacagt tcaatgctta ccgtcaggtg ctgcagatga
    14641 tggcagggaa aaaggtcatc atccgtaccc ttgatatcgg tgcggataaa aatgtagatt
    14701 acctcggact gggcaaggaa gataatcctg ccatgggata ccgtgcgatc cggatctgtc
    14761 tggagcagcc ggagatcttt aagacccagc tgagagcact tctcagggcg gcaaaatacg
    14821 gaacactggc cattatgtat ccgatgatca tttccgtgga ggaagtgttg gatattcaga
    14881 aaatcgtggc tgaggtggct aaggagctgg aggaagaaaa gcttccttac acaattccgg
    14941 aacagggaat tatgatcgag acgcctgcgg ctgtcatgat gagtgaagag ctggcggagc
    15001 atgttgattt cttcagtatc ggcacgaatg atctgacaca gtatactctg gctatcgaca
    15061 ggcagaacaa cagactggac agattctata atccacatca tgaggcagtc ctgcgtatga
    15121 tacagatgac tgttgatgga gcacataagc atggcaaatg ggtcggcatc tgtggagaac
    15181 tgggagcgga tacgacactg accacacgat ttgtgcagat gggaattgat gaactcagtg
    15241 tggcaccgtc catggtgctg aatgtccgta gtaaaatatg cgaaatggag tgagagattc
    15301 atgagaatat acaaagcaaa agattatgca gatatgagca gaaaggctgc aaatatcgta
    15361 tcggcacagg ttatcatgaa accgaactgt gttctcggac tggctacggg atctactccc
    15421 atcggcctgt ataaacagct ggtggaatgg ttccgcaagg gagatctgga cttttctgag
    15481 gttatgacgg tgaacctgga tgaatataaa ggtctgagcc gggaaaacga tcagagttat
    15541 tactatttta tgcaccagaa tctgtttgac catgtgaata tcccggtgga aaatacacat
    15601 ctgcctaacg gaatggagcc ggattcggag aaagaatgcc gtagatatac ggaattgatc
    15661 cagtcgctgg gcggtgtgga tctgcagctg ttgggaatcg gacataacgg gcacatcggc
    15721 ttcaacgaac cgggagagag ttttgacaag caggtacatt gtgtgaacct gacagagtct
    15781 acgatcgaag ccaacaagag attcttcgca tcggctgagg atgtcccgaa gcaggcatat
    15841 accatgggga tcaagaccat tatgcaggcg aagaaaatat tgatcgtagc cagcggcgaa
    15901 gacaaggcgg agatcgtaca gaaagctttc ttcggcccca ttacaccgca ggtgccggca
    15961 tcggtattac agcttcataa tgatgtgaca ctggtggcag acgaagcagc gctcagtaag
    16021 ctttctgaat aagaaagggg aagctgatag gtcatgagaa tcagaaatgc aaatatatat
    16081 acagaggagc atcggtttgt cagaggggat gcagccgtgg agaacggcag gttcgtaagc
    16141 tgtaccgggg atccggcagg tgaggaagct gttgtggatg cggaaggcat gtatctgata
    16201 ccgggactgg tggatattca tttccatggt tgtaagggcg cggatatgtg tgacggtacg
    16261 aaggaagcac tggatgtcat cacatcatat gaagcctccg tcggtgtgac aagtgtctgt
    16321 ccggcgacca tgacgatccc taaggatgaa ctgctggagg tcatgaaaaa tgccggcagc
    16381 tatgagtatt ccggtggatc ccacctggtg ggcatcaata tggaaggacc ctttatcagt
    16441 ccggcgaaaa agggtgctca ggcggcggaa aatatcatgc actgcgatta tgaatatttc
    16501 agtcagctgc agaaagcggc gaacgggctg atcaagctgg tagatatcgc accggaagag
    16561 ccgggagcga tggaatttat cgacagagcc aaaggagaag tggtcatttc actggcgcat
    16621 acagcttccg actatgatac agcaagggaa gccattgaac atggagcatc gcatgcgaca
    16681 catttgtata atgcaatgcc gccgctgaac cacagggagc ccggcgtgat cggagcagtg
    16741 agagattcag agaaatgtca tccggagctg atctgtgatg gtgtacacat tcatccgtca
    16801 gtgatccgtg caacatttgc gatgtttggt gctgaccgca tgatcctgat cagtgacagt
    16861 atgcgtgcaa caggcctgga ggatggtgaa tatactctgg gaggacagcc ggttactgtc
    16921 agaggcaatc tggctaccct gcatgacggc acgatcgccg gatcggcgac gaatctgatg
    16981 gactgtatgc ggtttgtagt aaaaacggtc ggtctgccgt tagagacggc agtgatgtgt
    17041 gccacggaga atccggcgaa ggagatcggc attttcaatg aagtgggcag tatatccgtt
    17101 ggtaaaaggg cagatttcgt actcctggat caggaactga acattgcagc ggtatatatc
    17161 gacggaaagg agttcacatg ttagattttt tgaaaggtaa gcgaaaaggg aattgtatcg
    17221 gatctccctg taaggggaag gcagttgctc ttacggaggt accggatcct acattttccg
    17281 agaagatatt gggagacgga tttgcagtga tcccctctga ggggaaaata tatgctcccg
    17341 cagacggaga ggtgaccgtg gtatttgata cgcttcatgc gatcaccatg acaacagatc
    17401 agggagcgga gcttctgatc cacatcggac tggacaccgt gacacttaag ggagccccct
    17461 tcacagcaca tgttgctgcc ggagaccatg tcaaaaaggg cgatcttctg ctggatgcgg
    17521 atctggagaa gattcaggaa gccggactgc atatcatcac tccggtcctg gtctgcaata
    17581 ccgatgaata taacaagatc agtattctga aagagggaga ggtatctccc ggcgaggacg
    17641 tgctccagat ctcttaagag cggtacgtcc gtatcaacta acgaaactga ccggaactgt
    17701 tccggttgag tataatgtaa aggaggagaa atgtatgaag tttcttcaaa aactgggtaa
    17761 agcgttaatg cttcccgtag cggttctgcc gatctgcggt atcctcatgg gtattggtta
    17821 caggttgtgc ccggcaacaa tgcagggtgg tgagatccag ggcgtggcaa atctgatcgg
    17881 tctgttcctc gtaaaagcag gtggtgcttt gatcgacaac atggcgatcc tgtttgcaat
    17941 cggtgtaggt gtcggtatgt cagagaaaaa tgacggtacc ggagccatcg cagctctggc
    18001 ttcctggctg atgctcacaa cacttctgtc taccggattt gttacgacca ttatgccctc
    18061 catcgctgag gatgctacca agacactggc attcgataag attgcgaatc cctttatcgg
    18121 tattattgcc ggtattatcg gttccatgtg ctacaacaag ttcaaggata ccaagcttcc
    18181 cgactggctg tccttcttca gtggtaagcg ctgtgtagct atcgtagcag gtgtggtatc
    18241 tattctggca tctgttgtac tgctgttcgt atggccttta ctgttcggcg gtctggtagc
    18301 tctcggtgaa gccattgtag gtctggatgt agtcggtgcc ggcatttatg ctttcctgaa
    18361 ccgtctgctg atccctaccg gattacatca cgcactgaac aacgtattct ggttcgatac
    18421 catcggactt ggagatctga agcacttctg ggcaggtgag acaagcgctg atgtatcctg
    18481 gagccttgga atgtatatgt ccggcttctt cccttgtatg atgttcggta tccccggtgc
    18541 agctctggca atggtaaaat gtgcaaagcc cgccaagaaa aaggcagcga tcggtctggt
    18601 agcttccgca gcaatctgtt ccttcatctg cggcgttacc gagccctttg agtttgcatt
    18661 catgttctta gctcccggac tgtatgtgat ctatgcagca ctgtacggca tcttcaccat
    18721 gatcactgtt gctcttggct tccgtgcagg cttcagcttc tccgcaggtg cgatggatct
    18781 gtttttctca tcctccctgc ctgcagcagc cagaatctgg ctgatcatcc ctctgggtat
    18841 cgcagcattt gttgtattct acttcgtatt cctcttcgct atcaagaaat gggatctgaa
    18901 gacccccggt agagaagatg atgatgtaga agcagagaag aagatcgagc tggcaaatga
    18961 tgatttcgca gcaatcgcag caaccattct ggaaggctgc ggcggcaagg acaacattgc
    19021 aagcattgac aactgcatca caagactgag actggaagta aaagacatta ccgcagtgga
    19081 tgataaggtg atcaaatctg caggcgtagc cggtgtcatg aaacccggta agacctctgt
    19141 tcaggtaatc atcggaccta aggttcagtt cgtagcggat gcattttcca agctttgcga
    19201 ataataaagt actgataaaa ctgaatatat gaacggaaga gagacctgca tgggaagacc
    19261 atgcgggtct ttcttatata tgatgtcggg gtcaaaagcc cccgactttg atgcctacgg
    19321 attgttccgt gctacgcact cccacaatcc taccctctat tcgcatatcg tgggattatc
    19381 atcccacttt tatgctcata tcggggcggg tcataaggtc tttcacccac ttatgagcaa
    19441 gctcatgtgg gcttagacct tttgaccctg gcatcatata tatttaataa ttactttatt
    19501 gacatttact ttggaaatga taaaatcatg acggagaaaa tcagtgggcc tgatgcctgc
    19561 ggaatgtatt gtaatgtaat ggagagaaaa gtcatgaaaa aatcaaaagg aaagaaggga
    19621 aacgggggat tttcattagt agagctgatc attgtaatag ctatcatggc agtgctggtg
    19681 ggagtactgg caccgcagta tctgtcttat atacataagg caaaggtggc ggcagatcag
    19741 gcgaatctga agaactattt tacggagatc cagctggatt atgcttctac aggagtgtat
    19801 aatccgcagg tgccgctggt ggataaatat ggagaaggcg gttatgaact cagagagatt
    19861 cactttttaa gcgggcagac tgtgaagctg aaggcaggat actatgctgt gacagaaaac
    19921 accaggggtc agggaggtta caatatatgc tattattgtg atctgtgcac agctgctgat
    19981 gcggatgaac agacaaagca aaagcatctg gatacttgtg tcattacatt tctgtgatgg
    20041 cggctgatag gaatatgtta tttttccaga cataacccaa tattatcaga aatgcagcag
    20101 atgtatccat gcttcccggc gtaggcggct gcctgtctgc ggatggcagg aaccgttgcg
    20161 tattccaggg tgtaagcggt acagccgtgt gacatagcaa aatctaaata atcagtatag
    20221 tattcacgtg tacttttatc gtttatggta tagatatcct ttgaaaaatc atacgcagta
    20281 aaaacgttct cctggtttac cccatcgatg agaacgtttt tttctgttgt aagatatttt
    20341 tttacaaaac agtccccacc gttgatcatg atctttctgc cggtatggtt catatagtcc
    20401 agaattgtaa ggattccgtc atagatggat tcctgcggat agttatagta tacatccgtg
    20461 ttatcgacaa aaaagccgtc cacacccttc tgcaccagag agtctactct ggaagctgta
    20521 catgcctgcc attccggagc cgagacatcg atccattttt cctccggcca gttctcataa
    20581 gcgcccagtg tatatttctc aaagtctgta tcataggatc ggaagctttc gatcgatccg
    20641 atattgatat aggagtagat ttcacggaca ccattttctc ttaaacgggc aatgtcattt
    20701 tgggaaaaat attccgcatc tatgacaagc agatcaatgt cagaaagttt ttctgtgatt
    20761 tcagaatctt caccgatcag gacaacgtat tttcctgtac tatcggaggt gctggcagag
    20821 caggcactga gcagggctgt agataatatc agaatgagaa cacatattat ttttatgaat
    20881 agcttcgttt tcctcaatta acggtacctc ctttttcgct tgatctgctt caatatttgt
    20941 atccagataa ttatactata gatggctcta catttcatgt atttctgtta actattcaga
    21001 gccatacaaa atactgcatt caggcagata atgtcgaaaa aaatatctgt aaaaatatta
    21061 aaaagggcga aaaatattga aaaatgtgat ataatttttt atgcaaatat aagataatac
    21121 cgggacaaaa agcccccgga tttgatgcgt tcagcggtta agacaaccgg gaaagtagga
    21181 agttatatga agattcagga tttttgtgac atggacaaat ttgagcaaat catgaagaac
    21241 tgggcaagca gtacaggact ggcaaccgtg gctgtgggtg cagacggaaa atacatcagt
    21301 gactgttata attttacgga gttctgtatt gatctgacga gaggaagcgc agaggggaaa
    21361 aagcgctgcg agcagtgtga cagagaaggc catggtgtgt atccctgcca tgcgggactt
    21421 gtggatttcg gtattccgat cacactggag gatggaaccg tgttgggcag tattatcgga
    21481 ggacaggtgc tgccggagaa tccggatgag gagaagttcc gtcagacagc aagagaactt
    21541 ggcattgatg aggataaata tatcaaggcc ttgaaaaagg taaatgtaaa gacaagggag
    21601 cagatcgacg cttcggcaaa tctgctggga gatgtgatca acatgttcgt aagagccagc
    21661 tacgtcaaca ggaaaaacga gaatctggta ggtgagctaa agggtggcat tacgaaagct
    21721 gcggaacaga tcgaggaagc aacggacaag accaaggaaa tcgatggcta cagcaagaga
    21781 cagcagattc tggcactgaa tgcttcgatt gaggctgcaa gagccggaga tcagggcaag
    21841 ggctttgctg tggtagcaac ggaagtacag aagttagcaa gagatatggc gacctccagt
    21901 gcggacatta agaagctgtt gggagagctg catgttacga tcaaccattt gaatcaatag
    21961 taatttagga taaagtttac aggaaaaccg tttttctgat ccacaaaggc gcagaaaggc
    22021 ggtttttttg cacatttttg catgtttcct tttggtgtgt ataaaaaagt cggagaagtt
    22081 gtccgatatt ctcaatagag gaaccaggta aacaaacgga tttggcattt attttcaagg
    22141 aggaaataat tatggcggga atcggagcaa ccggcagcgt aggtgcgtat gctgcgcaga
    22201 gtgattacag caagagtacg aagacgaaga agcctgaggt attcggaacg ccggataaga
    22261 cagccggcac aacaggtacg ggaaagacgt ccgaaacggc acagaccagg ggaaagactg
    22321 aggattacgg cagaaccatc ggtcagccgg agctgtcgga gaaggctgcg aaatactatg
    22381 agcagctgaa gaagaggtac ggaaattatg attttattct ggtcagcaga gatcagaagg
    22441 agaatgcaaa agccaatgct gcaaaatatg ccaatggcta taagacggtt gttctcattg
    22501 atgaggacaa gatcgaacag atggcaacag acgagaaatt ccgcaaacag tatgagggaa
    22561 tcctgagcgg tgccgctgca cagatacaac agttaaagac cagcctgcag agttccggtg
    22621 cgcaggtcaa gggctttgga atgcaggtaa atgatggagg aacactgtca tttttcgctg
    22681 tattgaagaa atccagcgcc gagcagaagg cccggatcga gaaaaaggct gagcagaaga
    22741 aaacagagaa aaaggctgcg gagaagaagg ctgc
//
LOCUS       DAWEJW010000039        22762 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_916_length_22762_cov_57.700181, whole genome shotgun sequence.
ACCESSION   DAWEJW010000039 DAWEJW010000000
VERSION     DAWEJW010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 22762)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22762)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22762
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_916_length_22762_cov_57.700181"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2297..2396
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5244..5343
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16079..16178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19599..19698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4794 a         6762 c         4669 g         6137 t
ORIGIN      
        1 cggaacaatc cgtaggcatc aaagtcgggg gcttttgacc ccgacatcat ataattatat
       61 ggtaactatt cagagccatg caaatcatgt caacatgtac gtcgaatgct tgcattcgta
      121 agaacatttg atatgattta tatggcgaag taaccacaat gagcagagaa caggccggcc
      181 tgttctccaa ggaagcctca tagaggcgat tttgtgaatg ggggtctgaa tagttacatt
      241 atttaggtag aaatcataaa gaaggtgatc cgtcatgtcc acaaagaatt ccaacttttt
      301 ttccagagat tgtatggtac aggctctgat ccagctgctg aagaccaaat ccctgtccaa
      361 tattacaatt accgagctga ccgagcgtgc cggtgtctcc cgcatgacct attatcgcaa
      421 ttaccattct ctggatgaga tcttcagcag ttatctgaag gatctggtag aatcctaccg
      481 tcaggatgtt gcgacatggc cggacaaggg caattacaat gactcccgca atatgctgca
      541 ttgttatgaa tatttcaatc attacaaaga attcattgcc tgtctggtgc agaccggtct
      601 gggacacctg ctgttacagg cactggacag ctacattctg gatacctatt ataccgaaga
      661 ccaggggagg gatttttatt acacgcttcg agccttctcc ggttccctct acaatattta
      721 tgtcacctgg attctggagg attccaaaga atccgctgaa gagatcgtat ccattatccg
      781 caagatctat tcgtaggttc cccgatctgt tctccatagt aattcagcaa aggactgcgc
      841 aaaatacgca gtcctttatt atttgataat ttatctctga tccaaaggaa catattccag
      901 ttcctgcatt acgctcttgt aagcgggacg aatgatctta tccatattga tcagctcttc
      961 gatacgatgt gcactccatc ctacgattct ggcaatggcg aagatcgggg tatacagttc
     1021 cagaggaatc tccaacatgc tgtatacgaa accgctgtag aaatcaacgt tggcgctgac
     1081 acccttgtag atccgggatt tctgggaaat aacctccggt gcgatccgct cgatcatgga
     1141 atacagggcg aaatccttgt ctctgccctt ggctaccgcc agtttttcca cgaagctctt
     1201 gaatacctgg gctctggggt cagacaggga gtagactgca tgccccatac catagatcag
     1261 tcccttgccg tcgaaggctt ctttgctcag aatcttgttc agatatgctc ttaccgcatc
     1321 ctcatctgtc cagtcggaca catgtgcttc gatatcccgc atcatctcta cgactttgat
     1381 attggcacca ccatgtttgg gtcccttcag ggaggaaagt gctgccgcaa tgacagaata
     1441 agtatcggat ccggaagaag tcacgactct ggtggtaaag gtggagttgt taccaccgcc
     1501 atgctccata tgcagcacca gtgcggtgtc cagtaccctc gcctccagtt cggtgtattg
     1561 tttgtccggg cgaagcatca tcagcagatt ctctgcggtg gaaagtttct tctggggacg
     1621 gtgtatgtac atactctcat cgttggaata gtgattatag gcatggtagc cgtagactgc
     1681 cagcatgggg aacacagcaa tcagtcccag ggactgtctt aatacatttt caatggaagt
     1741 gtcgctgcag tttttatcat aggaagccag tgtcagaacg ctcctggtca gtgaattcat
     1801 aatatcactg ctgggggctt tcataatgac gtctctggtg aagttggtcg gcagagttct
     1861 gctggacgca agaatatcgt taaaatgttt cagttcctca cggttcggca gcttaccgaa
     1921 cagaagtaaa taagccactt cctcgaatcc gaaatgatca ccccggaagc cgttgaccag
     1981 ctcgatgcag tcatagccgc gataccacag cttaccttca cagggaactt ttttgccgtc
     2041 ttccattttg aaggattcga tccgtgaaat attggtaaga cctgcgacta cgccgttacc
     2101 gttcttatca cgcagtcccc tctgtacgcc gtattcgtcg aacatctggg gttccaatct
     2161 atctgccgct ctgcacaatc cggcatgttc caacatatac tcttcgtttt tcttcatcca
     2221 aataccttcc tttccctcac atttttatat acttatttat cataactttt tggaaggggc
     2281 gatgtcaatt aacaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatta
     2401 tcccagaatc ctctttgtat tttcttttat tttctccgac agatctgcgt attttatcag
     2461 ttcttcctcg gtaaatcccg caaatctcgc ctcatcataa tcccgctgtg ccagtgccag
     2521 ttccttcatg accgcttccg cagtgggaag gattgtcact gtgaccttgg ttttacggtc
     2581 tttgcgcctt gtctccggct tttccgcttt tccctgtact tctccggctg ccttgctttt
     2641 tctggaaggc ttgggttcct tcacttcctc tattttcaag aatcccttca tggtcaggcg
     2701 ttgcaagccg ctggtcaggt tcgtgcggga gattccggcg aaatccgcca gttcccgtct
     2761 gctgtcccac tcgtgctttt cacacaggca cagaagcagc cgcacatccg ctaccttcag
     2821 atctcctttt aatgccaccg gctccagata atgttccaga agcttccgct tacggtaagc
     2881 atcccccgcc ggtccctcgg cctgcaggat ctcggtattg acaaagactc tctcgtctcc
     2941 cagcttcttg gatccgggca ggatggaagg cggcacggtt ccgtctcctg ccgcatcaat
     3001 gaagaaacga tatacctgca aacggttctt ctcgtagtcc tcttcttcca gcacatgctt
     3061 gtagaagtta ttgtaatact caaagacatt caacttcatc tttaccgtct tggagatgct
     3121 gattccctga ctggcaaggg agcctttgcg cttcacataa taatagattg gggcatgcag
     3181 ggcatagaat actttcgcgt aacggatata ttccagattg aacagaaaat cctcacacca
     3241 gttgatattc gtatccattc gcagactgtg ctcctccacc agatccctgc ggtacagttt
     3301 gttccacaac acaccatagt aaaagtctgc cggattctcc atcatatgtg ccgcgaactc
     3361 ctcctgggtg agcacaccct cctcttcaat gtctcccttg ggggacagac gctctcccac
     3421 cacgcggtaa aagtctgaaa tgaccatatc gcagccatac tcttccgccg cccgcacgaa
     3481 cagtcttgtg gcatccggcg tgatccagtc atcgctgtct aagaattgca gatattttcc
     3541 ccgtgcacga tccaatgcca gatttctgct gtcggagact ccggtgtttt ccttctggat
     3601 cacaatgact cttgtgtcct tgtttccgta ttcctcgcag atgtcaccgg aggaatccgt
     3661 actgccgtcg tttaccagaa acacttcaaa gtccgtgtat tcctgattca aaatgctgtc
     3721 gacacagcgt cgtaaatatt gctccgcatt gtataccgga acaatgatgc ttaccatggg
     3781 acgcataagc tcatcctttc tgaaattact tcataacaat aagttcgtac gccctgctgc
     3841 cgatgccaat cttctccgca tgctccagac aggtcttgta ctccgcattg ggagtggtat
     3901 tttcaaaatg atcatggtga tcacaaaagc cctcttttgc catggcatcc gtcagctggc
     3961 tgttcggcag cggtgtctgc ttcaggcagg catccacaca ggcctgatcc agcgccagag
     4021 gatcgaagga ggcgaacata ccgatattgg gcaggatggg tacatcattc tcccggtggc
     4081 agtcacagtt cggagacaca tccaccacca gagaaatatg gaaattgggt ctgccgtcca
     4141 cgaccgcctt cgcatactct gccatacggc agttgaaatc ctttgttgcc gcatcacaac
     4201 cgctatggat tgcgtcgaag ttacaggcac cgatacagcg tccacagccc aggcatttgt
     4261 tatgatcaat atgcatctta tgggtagtct cgtcgtatac cagtccctca ttggcacatt
     4321 ctctcatgca tcgtttgcag ccacggcaca aattctcatt gatctccggt ttgccctgag
     4381 cgtgctgttc cattttaccg gctctggatc cgcagcccat accgatattt ttgatagcac
     4441 cgccgaagcc cgccgtttca tgccccttga aatgattcag ggaaataaac acatccgcat
     4501 ccataatggc ccggccgatc ttagcatttt tcacatattc gccgccctct accggcacct
     4561 cgatgtcatc cgttcccttc agaccatcac caatgatcac aggacaccca accgtcaacg
     4621 gtgtgaaacc attctcccag gcacagtaca tatgctcgat ggcattctta cggctgccgg
     4681 gatacagggt attacagtcc gtcaggaaag gaattccgcc cagctccttt accacatccg
     4741 ctaccgcttt cgcataattg ggacgtaaga atcccagatt acccagctca ccgaagtgca
     4801 tctttatggc aacaaacttg tgatccatat ccatatcctg aatccccgcc gccttcatca
     4861 ggcgctttaa cttactggga agtccctctc ccagacgggt tctgaaatct gtaaaatata
     4921 cctttgattt ttccataact ttatcctccg tacctaaaaa gatattacga ttataacaca
     4981 tctttttttg tttttgagaa tttttctgca aaaaaggacg aacccaactc tcgccagccg
     5041 gtttcgtcct ttcaacgtat ttcatccgaa atacttcccc atttctttgt acaccgtaca
     5101 tcgatgtacg atgcaagtgt ttttttattt ttcttgcaca atttttgcag ataccgccaa
     5161 atatcctgct atttaaaacg atttttgtac tgcgcagcct tttccacaaa ttgctctgcg
     5221 aactcggggc aggaaggata atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnaggaatg tccttctctg tctgtcaggg acgtatgcag gtataaaaag cctccgcact
     5401 ccgctacggt ggggaggcca cctatgactg ctgcccggat ggccttgcgc atggcattgt
     5461 tctcactcag ctgtccggca tataattccg gataaccgcc gcccaacagc atcccatgga
     5521 tattttccgg caacgccttg tcatgcaatg gagaaaaata caccagttcc gctccggtct
     5581 gttccagcag gcgcagattc tcttcataat aaaagcagaa ggcttcatcc cgggctacag
     5641 cgatcctcgg gcactttacc ctatcgtttt ccacagaatc cggatcttgc tcttctgccg
     5701 tttcttcact gcgttccttc tttttggttt gggaggttct ctctattctc tcagctccca
     5761 gcggttccgc ctcttctgca atctgcaaca gcttcgaaat gtctacgctc tcccttagtt
     5821 ttgccgccgt gacccggatc tttccctgca gatcttccag ttctcccggc aggcacaggc
     5881 caagatgtct gctggcgatc tgtaagtcct tattttccgg gaaatatccc agcactgcaa
     5941 taccaagttc ctcttccgcc agaggcttca gaatctcata gtaaactgcc gacatacggt
     6001 tgaagatcac tccccggatc agatgttctt tatcataagc cagaaatccg gtgatcagag
     6061 gaatcaccga ttttcccatg ccttttgcat ccaccaccag tatgatgggg gtctgcgtca
     6121 cctttgccag atgataggag gagccttcct ctcttattcc gcccagcccg tcatagagtc
     6181 ccataacacc ttccatgacc acaagctcgt cttccctgcg atctctcaga aacagtcttc
     6241 tcgtgccgtc ctctccggtg aaaaaagtgt ccagatttct ggcaggaatc cccagcacct
     6301 gtctgtggaa catgggatcg atataatccg ggccgcattt acaggaagag atatcctctc
     6361 cgttttcctt aaaaatctgc aataatccac acgtgatcat agtcttgccg ctgccgcttt
     6421 tcggggcggc gatcatgatg cgtcgtatct ttttttcctg tgctccgatc atctctgcac
     6481 ttctccattt tccctgtttc cgttttcttc ttcggtcagc aacgctttct tcactttgtc
     6541 catagagcct gtttctccct ccctgaacgt aaatgagcag atccacaccg gattctccgc
     6601 ctgcggcaga tgatagtttc ccagcttctt cacccggttt acctgcatct gcaggatttc
     6661 ctcgtcctcc acaggaaacg cttccaatac ttcctgaagc tccgcaatcg tctccatgct
     6721 gatggcattg atcacaatgc gcatatgagg atttttccga tacaaggtct gtaaaatatc
     6781 cattaatctg cctccactgc cgccgataaa agcatgggtg ggcacaggca gttcttccag
     6841 tccctccggt gccagtgctt cgatgatctc catattgtcc atatggaaat gctctcggtt
     6901 cttccgaagc agttccaccg cctccggttt tctctcaatg gcatagacat gcaccgtttc
     6961 cgacaatccg gcgatttcca ccgccacgga tccggtgccg ctgccgatat catagactac
     7021 ggctccatct gtcaggtgca gcttgcaaat actgacttcc cggacttctt ccttggtcat
     7081 aggagttctg cgaagttttc cctccgtccc ggaagcatcc cgtaggaaac aggtgtccgc
     7141 tctgccgtgg gtcaggagtt ccggctgtgg atgaggattg cggatcaggc aggtatacag
     7201 accctcttcg gtgacctcca cacactgctc cggcatcagg atccgtatat tttcttccgg
     7261 ataggacaat tgatatccca cgatcacttc acaatctgtc agtcccgtct ccgtaagcat
     7321 ccgtccgatc ttacggagat cctcacttcc cgaggtcaac aggaaagtct tctcatgact
     7381 caccacagtt gtcggaagat ggttcagctt cttgccgtgc atgcttaaaa tggcggcatc
     7441 ctcatagctt tctcccaccc gggccgccag agaaaccacc gatgaaattc ccggcaggat
     7501 cctcacactg cctctcagtt gtcctgctgc aatcgcttcc tgtaattcca catacaattt
     7561 tctgcatccg ctgtagaatc cggtatctcc ggaaaacaga accgctaccc gaagtgtctc
     7621 ctgctgtgcc agttcctcgc tttcctgtaa tttttccaga tagggcagga tctgggcagc
     7681 catatagtag ggcttttttt ctattctggc agaatagtct gcaatcatgc gctccgcccc
     7741 cagcaggata tccgcagttt cgattgtctg ctgcacttct tttgtcatgc agtctctgct
     7801 gcccataccg atcccggcaa ggcagatttc cagacttccc tgctgcgaaa tcttacaatc
     7861 acagagttgt cccagttttc cacagattcc tgcaaacgaa tacgagtctg tccccggaat
     7921 attcccggca gcatccgatg tcttctgcgc agactcaatc acatacaccg ggattcccag
     7981 cattttcgct gcttccagct tctcctggca gcctccggcc cggccgctgt ttttcgtgac
     8041 catatggcgg atgtcgtact gccttaggat ggcagcgttc atctctgtgg aaaaaggccc
     8101 ctgcaatgcc aggatctgcc ggcctttgat tccctgctcc atgcacagtt ccagactctc
     8161 tcttcccgga aggatccgca catgcaggcg gtctttcaat ctgccggaag cgcaatacac
     8221 cgccagttcc ttgcttcccg tggtcagcag aatattgccc tctgtattct ccaaagcttt
     8281 cgcacaatct gcattgtttt caaaatattg gacatttgta acaatgtcat ctgcttctgt
     8341 aatcccgtcg attttccgtt ccagacgcaa gtaaatggga aaatgtgcat ctttttccaa
     8401 agatttcagt gtatccacag ctccccgcag attctccgta acgaccctgg cgtaagggtg
     8461 ggtggcatcc actacaatct catagccttc gtcatgcaga aacttctgca tctgctcccg
     8521 gtccattctg ccgcagtgaa ggtttacaat gggctgcccg gctgtctgca tcgcttttgt
     8581 gctttccgtc tgttcccgca ttacgatttc accgtattcc gttgccacgc ataccgtatg
     8641 tgcgattccg gcatccgcca gaatctccga caatctccgt ccttctgtgg ttcccgcaaa
     8701 tattacaagc tttttcactc tgccttttct cccttttctg cctggtagcc tcttctggtg
     8761 atcagcttgt cccccaggat ctccgtctcc gaattaccaa taaatacagt ggtgaacata
     8821 ttgacttccc ggtcccttaa ttcccgcagc gtacaggtca ccgcccgggt accttctctt
     8881 ccaatatttt ccacatatcc acaggggcgc ttcccttcta tggtttccag taaaatatca
     8941 caggctctct gcagataatc ttttcgtcta tggctggaag gattgtagat ggccatggca
     9001 aaatctccca ctgctgccgc ccgcagtctc ttttcaatct tctcccatgg agtcatcaga
     9061 tcgctcaggc tgataacgca gaaatcatga tttaaaggtg ctcccagtac cgccgcaccg
     9121 ctgctggccg ccgtgatgcc gggaatcaca agcagttccg tctccgggtg ctttgccccc
     9181 aactcgtaca tcagggatgc cagaccgtag attcctgcat cgccgctgca gatcagtgcc
     9241 acctgatttc ctgcttccgc ctcccggaag catagctcac agcgctccgt ctcctgccgc
     9301 atggaagtgg tcagcagctt tttccctgca aaacgttctc ccaataattt gacatatacg
     9361 gtataaccca caatcacatc tacctgctcc agcgcagcga tggcttctcc ggtcatcatt
     9421 tcttccctgc cgggacccat tcctatgata tagattctgt tactcatctg attttcctct
     9481 ttccatcatt tgcaagctct ccggatcctc tcatgcttcc atcacttgta ggtattgtcc
     9541 cgaggatgcc tagctacatg aaaaaatctt tgttttttct gactgtcctt ccgattggtc
     9601 tttacgatta catcgatact ttccatgctt tctcggcaat tgccacggtc atgccatcct
     9661 gtgcctgctt agctctgcag attcttccat ctgcccccgc cgccttcatg gcggcacgtt
     9721 cacacacatt gtccacaccc acctgtgact ttacaaaatc ggagccatga aaagttccgt
     9781 acaccgtctg taatttctgt gccggatagg tctgaaacgg gattctgtac ttttcgctga
     9841 attccagcag acaccgttcc tctttcttta catcaatgga agccagtgct gcgacctgtt
     9901 ctatggcaat gtcctgttct tccagtactt ttcttaaaaa ggcttccagc ttcacactgt
     9961 ccgttccttt tttacaaccc gcacccagaa tatatttctt cggcttcaac aacagttctg
    10021 ctgcctcttt tcgaaatatt tcttccgtgt tgtccgctat taggacatcc actttttcca
    10081 ccggaggata tgcacacagc ctgatctctg acggaatagt ctcgtccact gccagatgcc
    10141 ctgtctgtac ggacatggta attttctcgc ctgccagtat ttttgccgac acccgggcaa
    10201 tcccctcccg gttgtggatc cacagatcat ttcttttggc aaacaaatcc accgcaaagc
    10261 tgccatgcag gtctgtggct gtcgtgatca cagggacggc tcccagttct tcggccagac
    10321 ggaccgccag ttcattggct cctcccacat gcccggacaa cagcgggatc acatactgcc
    10381 cctgttcatc catgaccagt accgggctgt catgcagctt gtccgtgatc cagggggcaa
    10441 tagcccgaac cgcaataccg caggcaccga cgaacaccag cgcatgatgg gcagccatct
    10501 gctcaccggc ccagactgac acactctcct gtaccgctat tgtattcccg gtattatctg
    10561 tcggtacgtt actgtctttg ttcggaatca ccccgaatgt ctgcgttgta acgtccccac
    10621 tgttttccgc tgtctgcact gtcttttttc gggagcattt gctatataga ataatcgttt
    10681 cgtctccacc gcacatgctc ttcagaatcc gttccgccat cttctgtcct gttttcgtaa
    10741 agctgatcac actaatcttc acgctgctcc tcccggtttt cctctgtctg ccggacactg
    10801 ctttttgcct gacggaattc tgtggaaaaa tccgccgcat acagcctcga cttctgataa
    10861 tttccatggg taataacatc ccccaccagt accagcgctg tcttaatgat gccgtgttct
    10921 tccgctacct gtgccagtgt ctccaccgta cagatataag cctcttcctc cggccaggtt
    10981 gccttgtaga tcagggctgc gggggtgtcc tccgcataac cgcccgccat cagtctgcgg
    11041 ctcagttccc tcagcattcc cgtgcttaaa tagatcgcca tggaagcctg atgggctgcc
    11101 agactttcga tgctctcctt ctgcggcact ccggtccgtc cttccatacg ggtgatgatc
    11161 agggtctggg agacaccggg cagggtatat tccagattta acgatgccgc tgcgccgaag
    11221 caggcactga ctcccggaca gctctcataa gggatatgta atgcatccag tgcatccatc
    11281 tgctcccgca ccgcaccata gatgcttggt tctccggtgt gcagacgcac cagcatcttc
    11341 ccctgttcct gtgcctcctg cataatctgc agcacttcct ccagggtcag ttttgcgctg
    11401 ttatggattt cacagtctgt tttggcatat tgtaacagct ccgggttcac cagtgatccc
    11461 gcatagatga tgacatccgc ctgttgcagc atgtgcatgc cccgcactgt gatcagatct
    11521 gctgccccgc ttcctgcgcc aacaaagtaa accattcctc cgcctcctta cttttcgcca
    11581 atgctccgaa atcattggca tacatgatac attcgatcgc aagctttccc tgcgcccgct
    11641 tctccagata caataaaata cgctccatgg cataatccat gacttcctgt ctcctgccgc
    11701 actcgtccag aatccgcaca gcttcctctg tggtgacaca ggacaggagt ctggcagcat
    11761 ccactgccgc tacaccgcat tttacagcaa aagcggtcaa cagctccatc ctgcagtcgc
    11821 cctcccggga atgggtattc ataatacctc ccgccacttt gatgagcttc ccgatatgtc
    11881 ccgcaagaag cagacgcctg aatccctctt ccaccgccat atcgatcgtc agaccgataa
    11941 aattactgca ttttacactt ttatccagat catagtgata ggtatttttc agaaaatcca
    12001 gtccgtaatt gcccggagag atcaccacat cttctctccc cagtgcccgc tgttgccgca
    12061 gttccacgcg gatcgtatcc agaatagcct gtgtgctcat aggctccacg acaccgctgg
    12121 tgcccagaat agaaataccg cccacgatgc caagccttgg attaaatgtc ttctctgcca
    12181 acttttcacc ttccggcaca gagatctcca ccgacaggat tccacgataa tccagcacct
    12241 ggcacacctg taatacttcc cgttcgatca tttctctggg gacgtgattg atagctgcat
    12301 ttcccacggg ctgatccagt cccggcttcg tcacccggcc gacaccgatg ccgccgtcga
    12361 tcacgacctg tgcctgttca ggtgcatgtc cggttccatt cttcctaccg ggttcttctc
    12421 tggctttcct atccgttatt tccgcaaaag aaaccttcgc acagatcagc gcccctgtgg
    12481 taatatcggg gtcatccccg ccatcctttt ccacggcgca gctgacctcc tgctccttgc
    12541 ggcagatgtt gcggatcttg gctgtgtaag ggataccttt gggtgtctct atggtgattt
    12601 ctgttttctc ccttccggtc agcagcatat aggcggccgc cttggccgct gccgctgcgc
    12661 agcttcctgt ggtaaatccg taccgcatgt cttctacctc actgttacta tatcatgatt
    12721 cgcaccttcg gtgcttgacg ttgcttcgca actatcatga tcaaattcgc cgttcgccaa
    12781 tcatgcaagc atgttggctc tcttgcgctc attatatcgt gagctcagag ctccagttgc
    12841 cgatcctgcc agtattctgg ctctcctgca ttccttatcc tctgtctact ccgtctcgcc
    12901 ggttgtcttg tgtacctttc aaatcgttat tcaattcatt ctttttcagt tcatacagca
    12961 aggcattgca gatggccgct gccacgttgc tgccgccttt tcttccacga ttgatgatat
    13021 agggcacatc cgtctccata atcagctcct tggcagcttc cacattcacg aaacccacag
    13081 ggaccccgat aacgaatgcc gggcaccagt ccctttcctg catcagctca tagatctgta
    13141 tcaacgctgt aggcgcattt ccaacagcga agatcactgg tttttccagc ttcgaagcca
    13201 attccatgct gatagccgcc cgggtggtct tccgttcctt cgccatagcc gccacatccg
    13261 catccgccat atagcagtgg gcttccccgc caaaagaagc caacaccttc ttattgatcc
    13321 ccgccagtgc catattggta tccgtcacaa tatccgcacc gttttttatc aggtttttag
    13381 ccatctccac cgcgttttcg gaataggtca tcgtctccgc ataatcaaaa tctgcgctgg
    13441 tatggatcac cctgcgggtg acaggatcct gctccgcagg caaagtgatg ccccgctctg
    13501 ccagttctgc ggcaataatt tcaaagcttc gcttctcgat ttcttccggc aatacatatt
    13561 caatgctctt tttttccata agttaccttc ccccaaaatt cattttccgt cttccggctg
    13621 cttctattta ctgctttgca aagaaaacga actcattcag cagtaatctg cgcaagttcc
    13681 tgtaacagca cttcattctc ttcatgtttc ttcactgcga tccgataata gccttcaccc
    13741 agtccccgga aattctcaca gttccgaatc aggatacctt ttgcaagcag gctgtcatag
    13801 atgggctgtg tggtatacag caacaggaaa tccacttctc cggacaccac ccggatgcca
    13861 agcttttcca gtccttccca caaatattct ctctcgtttt tcacatattc cactgtgtct
    13921 tgcagatatt gctctgactg ctcagcgcag gcgatgcccg ctctctgggc aaacaccgac
    13981 aggttccatt ccggtagctg cctgcgtatt ttctcccgaa gctctgcgtt gctgcatacc
    14041 agatagccca gacgcacccc tggcatagca aagcttttcg taaaggcccg tgccagcagc
    14101 agattttcat actgtccgat ctccccaagc agaaactttg gctgttcccg gccggactcg
    14161 cagaactcta taaaacattc atccactact acggtgattc cctgctgcct gcaatgctcc
    14221 aacagttcct gcagatattc tctgcccatc agctgccccg tgggattgtt gggattcgct
    14281 acaaacagca tgtctgtatc cgctgtcagc tcctgtaaca gctcccgccc cggacggaat
    14341 ccggtctctt ccggcagata tacatatttt atatagctgc ctgctgcttc cgccacatac
    14401 tcgtatccgt aaaaggatgg cacgggaatc acaatattcc ggggcttaag tgcatgcaca
    14461 accgccagaa aaagttctga tgccccgttg ccaaacagca gatactcctt tgggatttct
    14521 ctgccacatt ctccggataa catccggctc acggcttccg tcagggccgc cgcagtaata
    14581 tccggatatt ctcccacatg ctgcactgct tcatgaatcg cctgcaccgc cgcatccggc
    14641 atccccaaag gattgccgtt taccgaaaaa tccagccgta tctgtctgct gtaaatatca
    14701 ccgccatgta tgttctgctg cattttcctt cctttccggt tcctgttacc ataggacttt
    14761 tcatgtcctc tatcgtctgc aaatcatcaa aagctttcac gcccggccaa actctgcttt
    14821 tcacactgcc accgcaatca ggatccccag acacagaatc tcacagatcc acgccgtccg
    14881 gttcatcaat cggttcgttc tgcggatatc ctccggttcc actgcccttg ttggatcccc
    14941 gatatagggc tttttcacga ccttgccaaa ataactggca tctcccgcca gctggattcc
    15001 cagcgctcct gcgcacacgg attccgtctg ggcggaattg gggctggcat gctttctgtt
    15061 gtctctgcgc cagatacgcc atgcctgctt tccgttatag cctttcccac tgataaaagc
    15121 cgtcccgacc atcaacagcg cgctgatcct cgccgggaga aaattcatca catcatccag
    15181 cttcgccgcg gctctgccga agtgaagata cttctcattc ttatatccta ccatagaatc
    15241 catggtattg actgccttat ataaaaatcc caacaccggg cctcccagtg cagtatacag
    15301 catgggggca atcacaccgt cggacgtatt ttccgccacg gtctcgatgg ccgcctttgc
    15361 cacgccttcc tctgtcagat gttccgtgtc cctgcctacg atcatggata ccgcttttct
    15421 ggcttcctcc aggctcccgg cgttcaattg tttccacacc ttgctgctct ccacctgaag
    15481 acacctcgcc gccagaatct ggtaggtcat gaccgcttct atgaccactc ccagtcccgg
    15541 atgcatcgca taagcaccga ataaaatcag tgccgacagc attcccgtaa acagtaaaac
    15601 gaccaccacc agaagttttc cctgtctgcg ctccaccccc tctctctcgg tcggattgcc
    15661 ctgtggattg cctgctcgca gtgtcttttc tgtcttcgct atcagccaac cgattccccg
    15721 gatgggatgg ggcagaaaat agggatctcc aaagagctga tccagtaaaa atcccgctcc
    15781 gaatgccaga atatgatatt tcatcaatat gtttctcctt ttgtaaattc tgacaatcca
    15841 gactttgaca gtgggtgaaa atccttgcac tccggggctc cccctcccat cgggaatacc
    15901 agaataccgc tgtaaccctg tccgcatttt gcctgatagt caaaatattc tcccccggtg
    15961 aaatgactga gcagcgccat gatggttccg ccgtgaacca ctgcggatac gctttgtatc
    16021 ccagcgtcct gtgctccaat ttcatcgctt ctttccaact ccgttttctc cgatcctgnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gtcctgtgct ccaatttcat
    16201 cgcttctttc caactccgtt ttctccgatc ctgtattgta atcaccctgc tcagatgcag
    16261 cacttctctc caagatcatt ttcatgtagt ataacatttt ttcatatccg gcaacgtttc
    16321 tgcgaatgaa ttcctctctg ctctcgcctt ccggaaacgg cagtgtaccg ccgctgtcga
    16381 tccatctctg atatgccgga tccccggaaa gttcctgata attgtgtcct tcgaatgcac
    16441 cgaaatccat ctccgtccac tccgggacaa aaatgggagt ctgccctgga tacaggatct
    16501 gtgctgtctc gaggcaccgc ttcatgggac cggaaaacag aaggtcagca gtcggatagt
    16561 ttcctcccgt cacagctttt ttcagcattc ctattccttc cgggctcaat gcttcatccg
    16621 tttttccaag ataacgtcct tctttattgg aaagagtcgc tccgtgccgg atcagggtca
    16681 ggcggatctc tatttgattt tctggccgat tccgcatacg actctcacca cctcatctgc
    16741 ctgcgccgcc aacccttcca gaattctgcc ggtgcattct ctgtaagttc tctcctccgc
    16801 ttccatgggg acgatgccgt tgccgatctc gtcacagatc agaatgcagt ccggatattc
    16861 cttatagaaa ttcgctatca ttgcctccgg atctgttccc tgtcgcaact gctcccggat
    16921 atatcggtgc agatgattga cgatcacctg cgggccagcc gtctgccgcc ctgtaggctg
    16981 ctctgtctgc tttccaggca ttgtttcaac cgcgctctca gttgcccatg tcattttact
    17041 atccgatgcc agcctcagtg ttccatccag aaccggcact tcctgcctgt cttcacgctg
    17101 aacgtaatgc tgctctacat aatgtaattt tccctgagca aagccgccaa taattaatat
    17161 catttccctc tcccatttca aatgtgcttc tgcaccactc tatccggcaa tacattttac
    17221 actgtcagca tcatatgttc tgcgattgct gccgcaacca ccacgcatcc ttcgcaaaac
    17281 gtcacaaaat accccgccgt atctccggtg atgccgccga actctttttt gctgcgatag
    17341 taataatacg caaaagtaag cagggccgct gccactgcga ttcctcccgt tacgaaagat
    17401 gtccacatca taaagccgat gcacaggatt ccctgtgcgt acaaagcagt ttttaccact
    17461 cttttatgcg ccttatccgc gaacagatac agcgtcccct cctgctttgc ggaagggaaa
    17521 ctcaccactg caacaccgct cagcactctg gacaggaagc ttccggctcc caccacgacc
    17581 agcgtccggc attccgtaat ctccgaaaag cctcccagaa aaatcagtcc atacagagcc
    17641 agcatgatca ccgcaaaggc cccgatatga gaatccttca gaatctccag tttccgctct
    17701 ctcggctggt aggaatgcag ggcatcacag gtatctaaaa atccgtccac atgaaagccc
    17761 ccggtgatca gaatcggtat cgccgttccc accaccgtat agcacaaggt tcccactccg
    17821 aaccgggcac agatccattt ccacagatac cacaggacac cgatcaccgc tcccacccag
    17881 gggaaaaagc agagtgcgta ccgcatatcc tcatccttcc actcaaactg cggcatcgga
    17941 atcttggaat acatggaaaa cgctatgaaa aaagatttga taatacgcat gcctgtttcc
    18001 tttctatccc cggctgtcct gccagttctg tgcttttgct cctggtgagc cgggtttgat
    18061 ccgctgtggg atccccgcta cgacttccac aacctcgtcc gcctccgcgg ccagcctctc
    18121 gtttaccctt cccagcgcct caatatattc tatagtagca ctgtcatagg caatgccgtc
    18181 ttcgaataca ttattggtca cgataaccag cggatccgcc tgttttctta agacttcaat
    18241 gccttgcaga atttttgcga tcactgtctc acgatccaca ggctgctccc tggaaaacat
    18301 ttcgttggcc gcaagattcg acatacattc cagcaatact gccgcctccg gctccatttg
    18361 ggaggcagcc tgctccagtg cagtaggctg ttcgatggta gtgaaacctt ttccctgccg
    18421 cagtctgtga tgcctggcca ctttggcctg catttcctca tcccagatct gcatagtagc
    18481 gatataatat tttttcttat caccggcagt ccgcagcaga taatcttccg cataagcgga
    18541 tttcccgctg ccgctgcccc ccaggatcaa tatcatcatg ttgcttctcc atccgttccg
    18601 tctttcagcc gcgccaatcg caaggtcgcg ctatcacgct ttccgtcact tcgtgactac
    18661 ccttgctcct ggcggaaatg ttagcgctta tattgttcca ctgcaatctc cgaaaatgta
    18721 gcgctctgtc cgtagatgct cattgccata tccagcagcg tgaacatcat gactgctccg
    18781 gttccttctc ccagtgccat tcccgcatgg atcactggag acagctgcaa agcatctgcc
    18841 agcttcactg ccgccggttc tttcccaaga tgggaaggga gcagatattc ccgcacaccg
    18901 gggaacagat gctccgccac caatgctgcc gccatgctga tcacaccatc caacacaatg
    18961 ggtatatgcc acaacgcgcc gccgatgcac ataccggtaa gtcctgcaat gtccagtcct
    19021 ccgactgtct gcaataccgt gaaggcatca gcatgccaca agtcataggt ttccaatgcg
    19081 gtgcggacaa cctgttgctt acgagtcagt ccctggtccg taagtcccgc acctctgccg
    19141 gtgacttcct ctgcttcgca ctgcaaaaga gctgccgtca ccgcactgct ggtagtcgtg
    19201 ttgccgattc ccatttcccc agtggccaga agtccgtaac ccttctcctt gcaatcctgt
    19261 accagttcga ttcctgtgac aatggcccgg acagtctctt cctccgtcat cgtcggttct
    19321 ttcaaaaaat ttcttgtgcc gggacgcacc ttacgatcca ggacgccggg aatggattcc
    19381 tcactattta ttccgatatc cacagggatc gtctccgcgc cgatgctctg cgccatcttg
    19441 cagacggagg atcctttccg tgccatggac tttgccaccg ccaacgtgac ctcctgcccc
    19501 gtctggctca cgccttcctc cacgattccg ttgtctgcac agaagatcag cacacctttt
    19561 ttccgaaggt cgatctgttc cgttcccagt atagctccnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19681 nnnnnnnnnn nnnnnnnnag atcagcacac cttttttccg aaggtcgatc tgttccgttc
    19741 ccagtatagc tccaatctgc gccgtaatgg tttcaaagtt gcccatgcca tccagtggct
    19801 ttgccaaagt gtcccaatgc tttttcacaa gctttctcac tgcttcgtcc ggcggcatga
    19861 tccggatctg tgccagttcc tgtccggtat aattataaaa tacgggcttc tctgagcatt
    19921 ccatagatct cctccatacg cagatattcc cgcagggtat ctgccagttt atcgtattgt
    19981 ctttccttaa tggtgcggta atcctcccag acgccgttat ccagagagat gcctttttcc
    20041 cgggccagag tctgtatcat atgcccggca atctcgcctt tgtcgaacag gccgtggatg
    20101 taggaaccgt agattctgcc ggtggagtca gcgataacag cttgtgtgat ttccgtgttt
    20161 ctcgtcgcat cgtctgcaca gaaagcgctc cgattgcttt ctgcactgtt tgccggtctg
    20221 gctgccttgt ccacggtacc cttcgcatcg ctgccatccc cgtcacaata caccgtctgt
    20281 cccatatgga tctcataccc ggcaaactta catcccgtaa gtccggagaa aataccctcc
    20341 acagcatcca gctttccatc tgtctggcag cgatgtttct ccttctgcaa cactgtccgt
    20401 accggaagaa gttccatgcc gcggatccgt ccgccttcct ccacgctgtc cggatctgcg
    20461 atctcataac ctaacatctg atagcctcca cagatcccga aaataggaac ttttcccgca
    20521 gcacgtttta ccgctgcctc cagtccgttc tgccgcatcc atttcagatc tcccatggta
    20581 tttttactgc cggggagaaa gatcagatcc ggcattccca gctctctcac atttgtcaca
    20641 taccggacac tgacctccgg catctgttca aacacatcaa aatccgtaaa atttgaaatt
    20701 ctgggatagt ggatcaccgc cagatcgatc ttccccacat gttttgcatc aaaacggtcc
    20761 gtcaggctat cctcatcttc cagcgtaagt tccatataag gtacagtccc cagcaccggc
    20821 acctgtcccc gttctgttaa catctgaatg ccgggatcta aaattgtgct gtctccacgg
    20881 aatttgttga tgatcaatcc ctttacccgc tcccgctcct cttcggtcag cagcatcagg
    20941 gttcccaaaa gctgtgcgaa cacgccgcct ctgtcaatgt ctcccaccag aagtaccggt
    21001 gcatccacca tggccgccat tcccatattt acgatgtcat tctgcttcag attgatctcc
    21061 gccgggcttc ccgcgccctc gatgacaata atatccgcat aggtctccag cttgcggaag
    21121 gctgccttga tatccgggat cagctccgtc ttgtgggcaa aatagtctct ggcggacaga
    21181 tttccccgta cctcgccgtt cacgatgacc tgggatccgg tatggctggt gggctttaat
    21241 aaaatgggat tcatgcacac cagcggttcg atccctgcgg cctctgcctg catgacctgt
    21301 gccctgccca tttccagtcc ttccttggtg atataggaat tgagcgccat attctgtgat
    21361 ttaaaagggg ccacccggta accgtcctgc cggaagatcc ggcatagacc cgccaccaag
    21421 agacttttcc cggcaccgga catggtgccc tgtatcatga ttacttttgc catcgttctc
    21481 tccatcccac agatgctact gacatcccgg gaatttcgtt tctctgtttt ctaacaccca
    21541 cgctgttgct ccgggctttc tgacttttct atctgcttcc ccagctgttc tgcatactcc
    21601 agaagttctc tctgaaaagt ctccagactg ccgaaattct cccggcaaca gataacacgt
    21661 ttgcacttat ctatcgtttt ctttgctgcc tgtatttttt cccgtgccac cggctcaaag
    21721 ggatgctctg cgatcacctc cgccgccagt gccttcgcca cgggataatc cagatcattt
    21781 tcatacagga tcccggtggc aaaggggatt cccgctctct gcagacgccg gtaagttttc
    21841 cgtccgtttc cggctccggc gatcacgaag acctgtggtg ttccttccgg cgccgccagc
    21901 tccatatcac tgctgccctc atcatagctt cccatccgga ttccatataa ctgtgtaata
    21961 tacccgtcgg tcatgacctc ctccggcgtt ccgatccggt ccaccgccct accgttcaca
    22021 cacaggattc tatcggagat tttctccgcc agttccagtt catgtaagga caggatcacc
    22081 gtcagccgcc tttctctgca cagcttttgc aggatggata aaaattccag tttgtgacgt
    22141 acatccagat acgaagtagg ctcgtccagg atcagaattt ccggctcctg gcagatggct
    22201 ctggccagca ttacccgctg tctctgtccg tcactgatcc ggtcaaacaa agaatcccca
    22261 atctctgcca cctgcaccag ttccatggcg ttttgcaccg ctgcatgatc ttctgcccgc
    22321 agcaagccaa agcgtccggt ataagggtat cttccagtgg ccaccacatc ttcacaggtc
    22381 atcagctcgg cccgcaggtg tttggtcatc accactgcca tttttttcga caattctgct
    22441 ccggtgagcg acttcatatc ctctccgtcc aaccgcaccg ttcccgcgat caaggacagc
    22501 tgtctcgcaa tactttttaa gatcgtggat ttcccggcac cgttaggtcc cagtaacgtc
    22561 aggatctctc ccttcctgag tgcgatctcc acttcttcga tcagaggctt tccgtcgtaa
    22621 cccacgctta ttttttccgt tgtaatataa taaggcttct gcgtcttcat aggtaagtta
    22681 tccacgctcc cgtctgcttc tggataccat gatccacaat actaccggtg ctccgaagat
    22741 ggccgtcact gtgctgatgc ta
//
LOCUS       DAWEJW010000040        22406 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_933_length_22406_cov_60.308577, whole genome shotgun sequence.
ACCESSION   DAWEJW010000040 DAWEJW010000000
VERSION     DAWEJW010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 22406)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22406)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22406
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_933_length_22406_cov_60.308577"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    20005..20104
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6294 a         4747 c         6059 g         5206 t
ORIGIN      
        1 gctgtgcttg cagacaaaat attgctagca cagcttattt ttgccttccg gacagcacag
       61 aaaagtgcag aaggtcataa aatcatgcta tttgatctat ggtattctct acttgtcaac
      121 aaaaacaaac caaacaaggg aggttacttt ttatgaggaa gaaattatta agcacacttc
      181 ttgcaacagc catggctgtt acaatgcttg caggatgtgg atccaatggg ggacagcagg
      241 caaccacacc ggcaacggac aataagacgg agacttccgc agaagcaaag acggagactt
      301 ccgcagaagc aaagacagag acttctggtg caacaggcgg cggtaaggtc ggtgtagcaa
      361 tgcccaccaa ggatctgcag agatggaatc aggatggtag caacatgcag gctcagctgg
      421 aggcagcagg ctatgatgta gatctgcagt acgcatctaa cgatattccc acacaggtat
      481 cccagatcga gaatatgatc aactccggct gcgaacttct ggtcatcgca tccatcgacg
      541 gtgactctct tggaaccgtt ctggagcagg caaaggaaaa agacattccg gttatcgcat
      601 atgaccgttt gatcatgaac tccgatgcag ttacttatta cgcaaccttc gataactaca
      661 tggtaggtac gaaacagggt gaatacatca gagatcagtt ggatctggac aatgcagcag
      721 gacctttcaa tatcgagctg gtaaccggtg atcccggtga caacaacgca agattcttct
      781 tcggcggtgc catggatgtg ctgcagccct acattgacgc aggtaagctg gtagtaaaat
      841 ccggacagac cgatttcgaa accgttgcaa ccgctaactg gtctactgag accgcacagt
      901 ccagaatgga cgctatcatt tccgctaact atgcagacgg aaccaagctg gatgccgttc
      961 tgtgctccaa cgactccact gcactgggtg taactaactc tctggaagct aactacaccg
     1021 gcgagtggcc tatcattacc ggtcaggact gcgataagcc caacgttaag aatatgatct
     1081 ccggcaaaca gtccatgtcc atcttcaagg atacccgtac tctggcatcc aaggtagtag
     1141 agatggtaga cgcagtcatg aagggcggcg aagcacctgt caacgatacc acaacctatg
     1201 acaatggtac cggtgtcatt ccttcttacc tgtgcgagcc cgtattcgct gatgcaagca
     1261 actacaaaga gctgctcatc gattccggtt actacacaga agcagattta cagtaatttt
     1321 ataaaataat atggatcctg cgggatcctt acggcaggcg ggcgcttccc gcctgctgta
     1381 ttcatgggaa agcaaaatag aacggtttga ttagataatg tggaaataag acttgggagg
     1441 aataccattg gcaaaaatct tattggaaat gaaaaatata accaaaacat tccccggtgt
     1501 aaaagcactt gacaatgtaa acctgcaggt ggaagaggga gagatccacg cacttgtagg
     1561 cgaaaacggc gcaggaaagt ccacattgat gaatgtattg agcggtattt atccgtatgg
     1621 gtcttacgaa ggggacatca tctatgacgg tgaggtctgc aaatttgata agatcaagga
     1681 cagtgaggaa aagggtatcg taatcattca tcaggagctt gcactgattc cctatatgac
     1741 gatcggggaa aatatgtttc tgggcaatga acggggacat aagtacaaaa tcaactggaa
     1801 tgaaaccaat gccagggcgg cagaactttt gaagaccgta ggcctgaagg aaggccccca
     1861 gacattgata aaggatattg gcgtaggaaa acagcagctg gtagagattg cgaaggcact
     1921 ggccaagaac gcaaaacttc tgatcctgga tgagcctact gcctctctga atgagacgga
     1981 ttccaaggca ctgttggatc tgatgctgga actcaagaaa aagggaatga catccatcat
     2041 catctctcat aagctgaatg agatttctta tgtggcagat aagatcaccg tcattcgtga
     2101 cggatctacc atcgaaacgc ttgtgaaggg aaaagatgat attaccgaga gccggatcat
     2161 caaaggaatg gtaggaagag agatcgccga ccgattcccg aagagagagc cgaagatcgg
     2221 agaggtcctg atggaggtca aaaactggac cgtgcatcat ccactgtatt ctgaccgtaa
     2281 ggtggtggat aatgtcaatc ttaatgtgaa aaaaggtgaa gtagtgggaa tttccggcct
     2341 gatgggtgcg ggacgaacgg aactggccat gagcctgttc ggcaaaagtt atggttccaa
     2401 catcagcggc gaactgtata tcaacggcaa aaaagtacag ctgaatactg tacaggaagc
     2461 catcaaaaat catctggcat atgtgacgga ggatcgaaag ggcaacggac tgattctgag
     2521 caacccgatc aagattaata caactctggc aaatctggat gccatcagca gacacactgt
     2581 tattgataaa gacaaagagt acaatgtggc ggtagactat aagaacaaac tgaatacgaa
     2641 atgtccaact gtggagcaga atgtaggaaa cttaagcggc ggtaatcaac agaaggtact
     2701 tttggcaaaa tggatgttca cagatccgga tatcctgatc ctggatgagc cgacgagagg
     2761 tattgatgtt ggtgcaaaat acgaaatcta ctgcatcatt aaccaactgg tggcagaagg
     2821 aaaatcggtt atcatgatct cctcggagct gcctgagatt cttggcatgt gcgaccggat
     2881 ctatgtaatg aacgaaggaa aaatagtagg agaactggcg ggcagtgagg caacacagga
     2941 gcttatcatg tccaagatat tatcagccgg taagaaagga gcattggcat gaataaaaca
     3001 aagggcctcg attttataaa aagatatacc atgatcctcg tactgatcgt ggttacggca
     3061 ttttttgcat ggagaaccgg aggagcaatc ctgcttccca tgaatgtcag cagtctgatc
     3121 tcgcagaacg catatgtgtt tattctggca accggtatgc tgttatgtat cctgaccggc
     3181 ggtaacatcg atctgtccgt tggatctgtt gtatgtttcg tcggtgccat cggagcggta
     3241 ctcatggtca acatgaaaat gaacatgtgg ctggctattc tgatcatgtt tgttatcggt
     3301 acactgatcg gtgctttcca gggtttctgg atcgcttatg tgagaatccc gcctttcatc
     3361 gtgacattgg ccggcatgct ggcttttcga ggtttaagca acgtagtcct gcagggtatg
     3421 accgtatcca ttaaggactg cactacattc gtaaatctgt tcggtggcgg tgctgactgt
     3481 tatattcccg atttcttcgg taatggtccc atcaatctga cctgtgtatt tgccggtgtg
     3541 atcgctgcag tgatctttgc agtgttacag ctgaagaata gagccgacaa ggtgaaaaaa
     3601 ggatacgaag ctcccagcat gggttccacg atcggcaaaa tcgtagtgat cgctgtagtg
     3661 atcctggcat tcagctatcg tctggcacag cacaaaggta ttcccagcgt attgatctgg
     3721 gtactgattg ttatcatggt atacggttac atcacttcca acacgactgt aggacgttat
     3781 ttctacgcag tgggcggtaa tgagaaggcc accaaattat ccggtatcaa taccaacaag
     3841 gtttatttcc tggcttatat gaacatggga ttcctggcag cgttagctgg tatggtaacc
     3901 attgcacgta tgacttccgc acagccgaca tacggacaga actatgaaat ggatgccatc
     3961 ggttcctgct tcatcggcgg tgcttccgca tacggcggaa ccggaactgt acccggagtt
     4021 atcgtaggtg ctgtactgat gggtgttatt aacctgggta tgtctctgat gggtgtcgat
     4081 cagaatatgc agaaggtagt caagggtctg gtacttctgg cagcagttat cttcgatgtt
     4141 gtatccaaac gtggtggaaa attaattgcc aagaattaat ttctacatat ctaatcaaaa
     4201 gggcccggtg catatgcgct gcaggccctt ttgatggtat tgggaggaga agagcatgaa
     4261 tacgggaaaa gaacggaaga agcgggaagg aaagcagaaa aaaggagtcg ggatcggagt
     4321 caagatcgtg gcgcttttac tgctgctggc agttgctgca ctcagctgta tgggagtgct
     4381 ggtgcagacg ctgcactcgg tcattgtaac taacgatgaa atcgtggccg gtcaggtggc
     4441 tgaacaggag aaaatctcgg agctttccag acagtttact tacattaacg ggcaggtgtt
     4501 gacccatgtc atgaccacca acagtgtgac aatggaaaat ttgtcggata agatcctgca
     4561 ggagatcact gatatggaac aacagatgac ggagttcgaa ggactgctgt ctgaggggga
     4621 tgcccggaga gaggcatttg acagtgcttc tgcggaactg gcaaaataca aaaagaccgt
     4681 ggagagtctg ctggtcacca gtgcagagaa taagacgcag gcctatgtgt ctgccacatc
     4741 gaatctgccg atgttcaatg aacatattga aagctatatg aatcagatgc tggagatcac
     4801 cggagagcag atggcacagg cacagggaca gatggagcag agtgcggcca gagtgcccat
     4861 gatcgtggcg gtggcaagtc tggtactgct tgcagtagtc gtggtgatca tgctgggatt
     4921 gcggatctgg gtcatcggtc cggtgaaata tgctacgagg caggtggatg aactggtgga
     4981 gggcattcgc aataacagag gtgacatcac cagacggatc cgggtgaaat cgggggatga
     5041 agtcggcaga ttatccatgg ccatcaatga tctggtggcg cagatgcaga ccatcataag
     5101 ggcgattacc gagagctgcg gacatatgga ggaaaaacag accggtatta tctccaatgt
     5161 ggaaaaggta aatgccaccg cggaggacac cacccagaag ctgggagtga tggctggtgg
     5221 catgcagtct gtcacggagg ctatcggagg ggtgcaacag gataccgggg tgctggatac
     5281 cacggtggag aacatgctgg aggtggcaca ggatggacgc aattatgcgg cggatatcaa
     5341 ggatagggcc ggaaaaatga aggcaacggc ggtggcgagt aagcaggagg caacccgggt
     5401 gatgaaggag atcgacacgg caatgacgga gtccatcgcc aacagcaggc agatccacaa
     5461 gatcacggaa ctgacggaag aaatcctggg aattgctgga acaacgaatc tgctggcact
     5521 gaatgcgtct atcgaggctg ccagagcagg agaagcaggc cggggattca cagtcgtggc
     5581 ggaggaaatc cgcaagctcg cagacagctc cagagagtca gccaataata tccaggagat
     5641 aagtaaccgg gtagtggaaa gcgtggaaga actttccgaa aatgctacaa aacttttgga
     5701 ttttatgaat accagagtca tgagagatta cgatgctctg gaggatacgg gcacaaatta
     5761 tcatgaggct gcggatcatg tggatgagat gatgaatgaa ttccgacaga agatcgatga
     5821 acttttgtcg gtactgcagg atgtcaatag tgctaactca cagatggaag caaccgtgga
     5881 ggacagtacg gaaaagctgg cggtggtaaa gaacaacaac cggggattac agcaggagat
     5941 gaaggatatt tcctatgcgg tggatgatct gaccggtgca gtgggacagt tgaaagaaag
     6001 tatccgctgt tttacagtgg tatagacaga aaaaataaaa tgcacgggat tcccgatggg
     6061 gaattaccgt gcattttgaa tgactgcagt tacattttag gaattttccg gagcgtgatg
     6121 cagctccaga gccagatagg aattgtggta ttccttgcag taggtgacat ctttgtcaaa
     6181 ccatgcggga gggacgaaag cctctgctgc ttctttactt ccaaactcca cttctgccat
     6241 gatcagaggg gcgaaggggg catcaaaaac atccagctcg atggtcaggg aataatctgc
     6301 cggaggagta ggaaagcctt ctttgaatcc gggatgctcc agagggatca gatagcgtct
     6361 tttggagatt acgctgccat cggctttttc ccgtaaatga taataagatg cctcgttcag
     6421 cggaagattg ctctcatctc tggccatcat gccgctgcct ttgtaagtga gataatattc
     6481 atcatcctgt tttctggctc tgaccacagg attataattc agatatacct gctcgatatg
     6541 atgagaagga tagctttcca gattgtcggg taaggatttc agggtaaact ttcgctcgat
     6601 ttccatagta ttaatgcctc gcttctgtaa ttaagactta aaaacacttc tttagtataa
     6661 catgattttc cttgaaaata cagtgaaatc tgcaaatatc tgcccatggg aattgaatta
     6721 cgcttccaaa ttgtgtatac taatcataac tgtgcaaagt cagagtatat gacttgcaaa
     6781 tgagacagaa aacaaaacgg aaagcgaggg aaaaccatga gtaagatagc gatttttctg
     6841 gcaaatgggt ttgaggaaat agaaggtctg acggtagtgg atatctgccg cagatgtgga
     6901 ctgaccattg atatggtgtc cattacggaa gaaaaacagg tgatgggatc acataagatt
     6961 ccggtgactg cggatatgac attgtcccag gtgaattttg aggaatatga ttgtctggta
     7021 ttgcccggtg gcggacaggg aacgaagaat ctggaggcct gtgagcacct gatgcagcag
     7081 atcgatgctt tttatgcatc gggaaaatac attgcggcaa tctgtgcggc tcccagtatc
     7141 ttcggacaca gaggaatcct gaagggaaag agagcatgcg cctatccctg cttcgaggat
     7201 catctggaag gtgcggctgt gactgcaggc cccgtggaag tggacggtaa cgtgatcact
     7261 tcccgcggaa tggggacttc cattcccttt ggtctggcga tcgccggtgt attctgtgga
     7321 caggataagg cagatgcctg cgcccacgct attgtatttc agccgtaagc agctgaagag
     7381 aaagacgagg acagaaaatg aaaaaagcgt taattacagg aattaccgga caggacggct
     7441 cttatctggc agagttcctg ctggaaaaag gatatgaagt gcatggactg atccgcagat
     7501 cttccatcag caataccggt cgtatcgacc atctgatccg ggaggacaag atcgttctcc
     7561 atggcggaga catgacggat tccaccagcc tggtgcgcat catcggagaa gtcagaccgg
     7621 atgaaatcta caatctggga gcgcagagcc atgtacaggt aagctttgag gcaccggaat
     7681 atgccggaga tgtagatgca ctgggcgtgc tgcggattct ggaggctgtg cgtatggtgg
     7741 gactggccga gacctgccgt atttatcagg catccacatc cgaactctat ggccgagtgg
     7801 aagaagtgcc tcagaatgag aacaccccgt tccatcccta cagcccctat gcggtggcaa
     7861 aacagtacgg cttctggatc gtgaaggaat acagagaagc gtataatatg ttcgcagtca
     7921 acggcattct gttcaaccat gaatccgagc gccggggaga gactttcgtt acccgtaaga
     7981 tcacactggc agccggcaga atcgcctgcg gtatccagga taaactctat ctgggcaatc
     8041 tggattccaa gagagactgg ggctatgcga aggactatgt ggaatgcatg tggctgatgc
     8101 tgcagcatga caagccggag gacttcgtta ttgccaccgg tgtacagcat accgtaagag
     8161 aatttaccac actggcattc cattatgtgg gaatcgaact ggaatggcag ggcagcggta
     8221 tccatgaaaa aggaatcaac aaggccaacg gcaaggtggt ggtggaggtc agcgaggcct
     8281 tttaccgtcc cacggatgtg gtcaatctgt ggggagatcc gacgaaggcc cgcacggtgc
     8341 tgggatggaa tccccagaga accagctacg aggaactgtg ccgtctgatg gcagagcacg
     8401 atctgaagct ggcaaaggct gaagcggtag tgttgaagga aaccgcggaa taacaggaga
     8461 aataaagaga ggaagtactg taatatcatg gaaaaagacg caaaaatata tgtagccggc
     8521 cacaagggta tggtaggaag tgctatttgt agagcactcc agagaaacgg atataccaat
     8581 atcatcaccc gcagccataa ggaactggat ctgtgcagac aggatgccgt ggaggaattc
     8641 ttttccaggg agaagccgga ctatgtgttc ctggccgcag ccaaggtggg cggcatcatg
     8701 gccaacagtg aggcactggc ggatttcatg tatgaaaata tgatcctgga gatgaatgtg
     8761 atccatgcgg catggcagaa caactgcaag aagctgatgt ttttgggcag cagctgtatc
     8821 tatccccgta tggcaccgca gcctatgaag gagagctgcc tgttgacttc tgagctggag
     8881 aaaaccaatg aggcgtatgc actggcgaag atctccggac tgaaatactg cgagttcctg
     8941 aacagacagt acggaaccga ctatatcagt gtcatgccca cgaatctgta cgggcccaac
     9001 gacaattatc atcccaccca cagccatgtg cttccggcgc tgatccgccg cttccatgag
     9061 gcaaaagtat ccggcgcgaa ggaagtagtc tgctggggaa caggaacccc actgcgggaa
     9121 tttctctatg tggatgatct ggcagatgcc tgcgtgtatc tgatgaatac ctacagcggc
     9181 gatgagacag tgaatctggg aaccggcaag gaactgacca tccgggaact gacggaactg
     9241 gtagcgaagg tagtaggtta tactggggag atcaaatggg atcccagcaa gccggatgga
     9301 actcccagaa agcttctgga tgtcagcaaa ctggaaagcc tgggctacca ttatacgacg
     9361 gaactggaag aaggtatccg tctgacttat gaggatttcc tcaataatcc catgcgtgcg
     9421 gaacgctaat ggttccgggc acgaacagcg caggcagaca gacggcccgg gaaaggatga
     9481 agtgaacaga atgaatgatt ttcggaagac agcacaggag atgctggaat ttataagaat
     9541 aagtcctacc tgttttcatg cagtggccaa tatcggaagg atgctggagg aagcaggctt
     9601 tcagcagtta caggaaaagg aagaatggaa actggaaaag ggcggacggt attatacgga
     9661 gcgcaatgat tcttccgtta ttgcgtttgt gattccggaa aaggaggtcg gcatcagggg
     9721 ctttcatatg gcagcagccc acagtgattc cccctgcttt aagatcaaag agaagccgga
     9781 attgaccgtg gaggagcatt atctgcgtct gaacaccgaa aaatacggcg gcatgatctt
     9841 gtccacctgg ttggacagac cactctccgt cgctggcaga ctggctgtga agaacgggaa
     9901 cggtatcgag ggcagactgg tgaatatcga cagagatctc tgcgtgatcc ccaatgtggc
     9961 catccatatg aaccgggaaa tgaacaaggg tgtggagtac aatccccagg tggatctgct
    10021 gcctttgctt gcggacgtat catttgacga atatgatgca catactacat acgatgcaca
    10081 gaccgcatcg gagaatgcgg aggaacagcc ggaaacagta gagaagccca cactggcagc
    10141 gctggcagca gaaacggcag gcgtggacgc tgagacgatt ctcggagaag acctgttcct
    10201 gtacaccaga caggaaggaa aaatgatagg agcaaaggga gaattcgtgt tgtctcccag
    10261 actggatgat ctgcagagcg cttttgccct gacaaaagct tttacggaga gtactccggc
    10321 agagtatatc aatgtctgtg cggtgttcga taatgaagag gtgggaagcg gtaccagaca
    10381 gggtgcagat tccacttttc tggaagatat tttacagcgg atcgcggaag gattgcaggc
    10441 ggatcacagc agctatctgc gctgggtggc ggacagcttc ctgatctctg cggataatgc
    10501 ccatgcggta catcccaacc atcccgaaaa agcagaccct gccaaccgtc cgtacttaaa
    10561 cggcggaatc gtcatcaaat atcatggcag ccagaaatac accaccgatg gtatttccgc
    10621 ggcaaggatg aaggattact gcgaacgggc gaaggtacct taccagacct atgccaaccg
    10681 ttccgacatt gcgggaggtt ccactctcgg caatatttcc accgcacatg tatcagtgtc
    10741 cagcgtggat atcggactgc ctcagctggc catgcattcc gctgtggaga cagcaggcat
    10801 gaaggatacg gaatatgcgg tccgggcgct gaaggaattc tggggcgaat gattttggca
    10861 gcgggaattc ttaaaaaagt ataaaaagcg tggaagcact tgaaagttct gcacgaaaag
    10921 tgcatattta ggatacagga aaagatacaa aaaagatacg aaagaaggct gaagttatgt
    10981 ttgaaatgaa accggaatac tatattggaa ttgacatgat cgatcaggag cataaacagt
    11041 tgtttgacta tgccagtgaa gcatacgaac tgctgcagga agagtttaca ccggacaagt
    11101 atgacaagat tgatgcaatt ctggaaaaac tgcgtgatta taccgtaaaa catttttcag
    11161 atgaagaagc gtatatggaa tccatccagt acaaaaagat ttttacccag aagattcagc
    11221 atcaggaatt tatcaacaaa ctggatgaat ttattgatca gcatgagaaa gatgaagaaa
    11281 atcaggatga gcagatcatg gatattctga attacctgac agactggctg atcaaccata
    11341 tccttcatgt ggatggacag attccgaagg aggcgtaaga cctgactttt ccggaatgat
    11401 ttcccacatg ggacataaga tgtaagaagc ggaagtaatg gggagtgccc atggaccaga
    11461 aactggaaaa catcctgaat cttgcattgg agaccccgga ggaagaacgg gaacagacgg
    11521 aaagtctgaa tgtcggttac agtgcggaga gccgcagctg ggagctgatc gtaaaatatc
    11581 atggcagtct ggacggactg cgggaacaga atattgtggt ggaagagctg attgccggat
    11641 atgcgatcct tacggtaccg gagacactgg tagataccgt atcggagact ccggagatcg
    11701 agtatgtgga gaagcctaag cggttttact atcagcagac agatccggac ggcgcatcct
    11761 gctttccacc ggtgacaaga agaacgcctt ttctgaatgg aaggggcgtt cttttggctg
    11821 tattggattc cggaatcacc tgggatctgg aggtgttccg caaggcagat ggcagtacga
    11881 ggatacggta tctatgggat cagacgatac catgggatca gacgattccg ggaaagcagg
    11941 ccgcatcacg ggagcaggag acgctgcgga atccgacatt gccacagaat cagataatgc
    12001 cggaggaaac gggcgatacg ggatatggga ggacaccgga tggatttccc atcgggactg
    12061 aatatacgga ggaggaaatc aaccgggcac tgaacagcag tgtactggaa cggtacggcc
    12121 tgatccccag cagggatctg accggccatg gaacggcagt ggcagggatt gccgccggga
    12181 gaagtgcgga cggtctctat accggggcag ccccggaggc ggaactgatc atcgtgaaac
    12241 tgggacttcc cagggaggaa ggttttcccc ggaccacgga gattatgaga ggcgttactt
    12301 atgcgctgcg gaaagccaga cagttaaata tgcctcttgt catcaatttg agcttcggca
    12361 acagctacgg ttcccacgat ggaagttctc tgctggagag gttcctggac aatgcttcgg
    12421 agatcggcag gacggtgata tgcgtgggca gcggcaacga aggagccgcc agaggacatt
    12481 ttgccggcaa tataacaagg gacggccgcg tggaactggc tgtgggcaat tatgagagaa
    12541 acctgaacat acagctatgg aaaaattaca gcgatgtgtt ccggataaga ctgcaggccc
    12601 cgggaggtga ggaagcagaa ttgtctacaa atatccaggg aggaaaatac acgctggaat
    12661 tggaacagac tagaatactg gtttatctgg gagagcctct gccctatgcg gtggcacagg
    12721 aaatctatct ggacatgatc ccggcagagg gaagctacat caatgcaggg atctggacaa
    12781 tccggctgga accggtagtg accgtgacag gacaatatta tctgtatctc ccagcgggaa
    12841 gcggaatcgg ggagagtacc ggattctacc gtgccacacc ccaggtgaca ctgaccattc
    12901 cgtctaccgc agccaaggtg atcaccgtgg gagcctatga ccaggtctat gatacctatg
    12961 cagatttttc cggcagaggc tatgcagaca gtacccgtac catcggcgtg gcagctgcgg
    13021 gactgaccaa gccggatctt gtggccccgg gtgtgaatat ccaggctccg gatgtgtacg
    13081 gaagctttac tcccgtgacc gggacctctt ttgcgacacc cattgtcagc ggggcagcag
    13141 cccttttgat ggaatggggc attgtacggg ggaatgatcc cttcctctac ggcgaaaaag
    13201 taaaagcata tttcaggaaa ggagccagac cactgagggg ggagactgaa tatcccaatg
    13261 accgggtggg gtatgggaaa ctgtgcgtgg cggagagtct cctggaaaat tagtatcata
    13321 aagaatgaga ttggaaacag ttccggaaca aaatgcggca aaacaggtta aataacttca
    13381 agaaacttga ttgttttgta actattgtat agaaatgagg aggcaatttg tgtaaaaaga
    13441 tgcattgaaa gctacatcac aagatgatac tataaacaag aacaatcgtt tgcgcatatt
    13501 gtgacaataa agaggtatga aaataacgga ttgcgcaagg atggggagga aagtgtgcct
    13561 gcaatctgta ggttgcaggt cacggaaagg cgtgatgaag gtatgaacaa taattctggg
    13621 aatgataaga caatgacgat ccatgatgtg gcgagggaac tgggggtgtc tgccagcaca
    13681 gtttccaggg cgatctccgg aaaaggccgt attggagccg ctaccagaga ccggattctc
    13741 gactacatag aagaacatgg cttttatccc aatgcggcag cacagagcct ggcacaatcc
    13801 aagaccaata atatcgccat tatcctgccg gaggtcaata cccttgtgga tatgcctttt
    13861 ttccatacct gtatgtatgg cgtggaagaa gtggcacagg ccaatgatta tgatattatc
    13921 gtggtcacga cgaacggaaa cgataccaaa ccattggagc ggctgatcca gaaccggaaa
    13981 gtggacggaa tgattcttac cagaacttat gagaacgaca aatacgttaa attcctgaaa
    14041 cagaagcaga ttccctttgt ggctgtgggg aaattcccgg atgatgatgt ggtacaggta
    14101 gaccatgata atgtgggagc ctgtaaggaa ctggtgacgg ttctgttcgc caaagggatc
    14161 cagaatattg cctacctggg aagcaatatg gatcagatgg taaaccgctg caggtatcag
    14221 ggatatgagg aagcctaccg caagggaaag cgcaagctga accaggatct ggtgtacacg
    14281 gatttaagca ataaagccat ggtggaaaag gcagtggaag agctgaaaaa gagcggtgtg
    14341 gagtgcattc tgtgtcagga tgactacatc tgcgatgagg tagtcagaaa gcttgcccgc
    14401 atgggtgtac gcatcccgga ccagatgaag gtggcatcct gtcattacag ccgcattctg
    14461 gaaaattatc cggttaccat aacctcgctg aagttcaata tcacagagct aggacggaga
    14521 tcctgtcagg tgctgttgga tatgatccat ggcaaaacag tacctgacaa aacacttttg
    14581 gactatgaaa tcatcctgaa ggagtctaca aaaggctgac aagagtgtga aaatcatgat
    14641 caggaaaaac gttttcctta attttgctca aaaaaattat aaaaaaagca ttttttttca
    14701 aaaaaaggat gtgctgaaaa ccaagtaaaa tcaagacttt caaagcatga ttgtgcaaaa
    14761 tgcacaattt taaatctcaa tatcagtcat tattataaat tgacaacata aggaaatgtt
    14821 gctaaactat tatcgtagac aagcggttgc gcaatgcgca caaacttgca aaacaaaaca
    14881 ggaggataac aaaatgaaaa agttagtcgc tttagcaatg tcactgacta tggcattctc
    14941 tctggtagct tgtggaaact ctgatgcttc tgcaaatgcc ggaacagata ccacagcaac
    15001 ttctgcagca gcaaccacca ccgaggctgc acagcagacc gctcccgctg agaagcagga
    15061 tgtatcttta agaatgtggg gtgctgagga agaccagccc atgttactga gcatgatcga
    15121 ttccttcaag gaacattacg cagattatgc aaacttcgat atccagcttg gtaccgagtc
    15181 cgaatccaca gctaaggata ctgttttaac cgatattgag gctgccgctg atgtgtattc
    15241 tttcgcatct gatcagctga tcgacctggt aaatgcaggt gccctgcagt ccatcgatga
    15301 aatggatgca gccctggaag cttacgcagg caagagcgta gctgatgtta agagcgcaaa
    15361 tgctcccggt tctgtagacg cagcaaccaa ggacggcaca ctgtatgcat tccctatgag
    15421 tgctgataat ggttacttct tatattacaa ttccgagtta gtaactcctg aagatgcaca
    15481 gacctgggat accttacttg cagcagctga gaaggctggt aagaaggttg gtatgactct
    15541 tgcttccggt tggtataacg cttccttctt ctatggcgca ggcttcacta ctgcactgaa
    15601 tgacgacggc agcactgcta tggactggaa cggcaccagc gctgatggcg ttaccggtgt
    15661 acaggtagta cagtctatgt tgggtattgc cggcaacagc gcattccttc cgatcgctga
    15721 tggtgatatt tccaaccaga ttgcttccgg tgacctgtgt gcagtaattt ccggtacctg
    15781 ggatgcatcc gcagctcaga ccgctttcgg tgatggctac gcagctacca agcttcctac
    15841 ctacacctgt ggtgacaagc agatccagca gggctccgta gcaggcttca agctggtagg
    15901 tgttaacaag tattctgcaa atgcaggctg ggcaactctg ctggctgact ggatcaccaa
    15961 cgaagacaac caggcagtac gtttcgcaga gagagagatc ggaccttcca atatcaacgt
    16021 tgctggttcc gaggaggttt cttccaatac cgctattgca gctctgtctg agcagtccgc
    16081 ttatggtgta gttcagatcg ttggcggcaa gtactgggat cctgccaaga cttttggtga
    16141 gaaggtagct cagggtcagc tgaaggctga tgatgaagca ggtatccagg cagccctgga
    16201 tgagctggta gaaggtgttt ccgtacccgc tgaataagtt tgggaagatg atctgttccg
    16261 gttctgtgac aaatgaatga tgaaaacgtt ctggtgcaga acgaatacag agaatagaaa
    16321 ggcgtcctga agctatgaac aataatacgt agtggatgga tgcctttttt acaggaaacg
    16381 tttccggtaa gcgggttcga ctcctgcctg caggtttatg taactggtca gatgaacgcg
    16441 ggaagatgtt cggaacagtt actaaaaatt tatattaata tgaagaaagg agagggcaga
    16501 gaatgaagaa gaaaaagagc ggaatcggcg gattcttcaa ggagttcggt gaagcagtcg
    16561 taaaaggaga tctgttcgta aaattatccc tggtctggat gggagcagga tatatccgca
    16621 gaaaacaaat tatcaaaggt attttaatga ctctgctcga agtggcagtc attgtattca
    16681 cctttggctt tgccacagag tatgtacgtg attttggaac tttgggaacg gttcagcagg
    16741 agacggtttt caatattgta accatgcaga atgagttcaa tgattatgac aactcattta
    16801 tgatcctatt gttctccgta atctcatttg tggtctggtt cacattcctg gtgatctggc
    16861 tgaagaatga agtgaccgtt taccggcttc agaaaaaagc acaggcagga gaacatatca
    16921 atacgtttgt agaagacttg cagcagttca agaatgacaa attctatatc acactgctgg
    16981 cacttcctgt catgggtgtt gttattttca cgatcgttcc tctgctgatc ctgatcgctg
    17041 ttgcgtttac gaactatgat cagggacata tgcctccctc cgcattgttt acatgggtgg
    17101 gatttaccaa cttcaagaat ctgttctcca gcggtggaat gaccattacc ttcggttatg
    17161 ccttccgtag gatcttgatc tggacgctgg tatgggcatt gtttgcaacc gctaccactt
    17221 acatcggcgg tattctgttg tccctgctga tcaataataa gagaaccaaa ctgcccaagc
    17281 tgtggagaac tctgttcatt gtaactatcg cagttcccca gttcgtctcc ttacttctgg
    17341 tacgtaactt tttcgctaac aacggcatca tgaacacgat ctgtgccaat gcgggaatta
    17401 cccagtggct gagagatatc ggcgcgatca gtaccagtta tattccgttt ttgtccgctc
    17461 ccggatgggc acatgtcatg atcatcctga tcaatatctg gatcggtgtt ccttatcaga
    17521 tgctgatcgc caccggtgtg ctgatgaata ttccttctga ccagctggag agtgcgaaga
    17581 tcgacggcgc tactccgatg cagagcctta ttcatatcac tatgccgtat atgctgttcg
    17641 tcacgggtcc ttctctggtg acggattttg tcaagaatat taataacttc aacgtaatct
    17701 atctgttgac ccagggtgta tataccacca caaatcaggc aatggcgaac tcccaggcga
    17761 aggaagtgga tctgctgatc acctggctgt tcaatctgac acagagctat tacaattaca
    17821 agatggcatc caccatcggt attattgtgt ttatcctatg tgcggtattt accctcattg
    17881 ccttcagcag attcctgaag ggtgacagag aggaggcgtt ccgttaatga agagatttat
    17941 taagacttta ttacataacc tgttactggc gtttttggca tttgtctggc tggtgcctat
    18001 cgtatggctg gtggtcacct ccttcagcgc atacaccggc atgaatacca gcaccttttt
    18061 cccgaaggaa tggagtctgg cgcagtataa gaatctgctt ttctctgcgg acagtgtggc
    18121 acagttcccg aactggttca agaacacctt catcattgca tgtgtggtat gtgtgatctc
    18181 caccgctttt gtactgatgg tggcctatgc gacctcctgc atgcgtttcc cggcacgtaa
    18241 gccgctgatg aacgccgcag ttatcgtgaa tctgtttccc ggtgtattgt ccatgatcgc
    18301 agtatatttt atcctgaaga cattgaacct gaccaacact cacctcggac tgatcctggt
    18361 atattctgcc ggcgccggac tgggttacct gatcgcaaag ggcttcttcg atacgattcc
    18421 gaattccctg cgcgaggctg cttatctgga tggagccagt gaggctacga tcttctggaa
    18481 ggttgttatc cccatgagcc gtcccatcat tgtatatacc gttatcagct ccttcctgtc
    18541 cccctggatg gatttcgtat atgcaaagct gatgttaaat gccggcatat cttcccagtg
    18601 gactgttgct atcggtctgt acaatatgct ggataagagc ctgatcaaca cttactttgg
    18661 tcagttctgt gcaggcggtg tactggtatc tattcctatc tctgtattat ttgtgatcat
    18721 gcagaagttc tatgtggaag gtatcaccgg tggcgcagtg aaaggttaaa ctgcttaggc
    18781 agtttaacct aggagaatcc atggaatgga ttctccggat aaatgtatga catggagata
    18841 aagagttaat ttatggataa atttatcaaa ggtatggatg tatcgacttt gccggaagag
    18901 acagctctcg gtgcaaagta ttatgacgaa ggcaaagagg gggacgcact ggagatcctg
    18961 aaaaaatacg gtgccaactc tctgcgcatc cgtctctgga acgatcctta cagtgaggac
    19021 ggcaagcctt acggagccgg taccagtgac tttacaaaac tggtggcact ggcttccgca
    19081 ggcaaaaagc tgggttacag ctatctgctg gatctgcatt acagtgattt ctgggcagat
    19141 cccggcaaac agttccctcc caaggagtgg gcatatgcag atgccaatgc actggagaag
    19201 tatgtgtatg attacacgaa ggatgtgctg ctgaaattaa agcgtctgga tctgcttccg
    19261 gagatggtac aggtaggtaa tgagatcacc aatggcctga tgtggcctca tggaaaatgg
    19321 gacaatgttg acaatatcgc ccggtttatc agtgcgggca tccgtgcggt gagagaagta
    19381 tccccggata gccgcgtgat gcttcatctg gactgtggcg gcaacaatgc ccgctgcaga
    19441 gaatggtttg atgcctatgt acagagagga gaggacttcg atgtcatcgg attgtcctat
    19501 tatcctttct ggcatggaac cattgaggat ctgacaaata atatgaacga tctctctcag
    19561 agatatcaga aggatgtcat tgtggcagag gtatccatgg gcttcaccat ggaggactat
    19621 gcagacaggg aaaaactgtc accggaacag cgcaagggca tggcgaccaa gccccacctt
    19681 gcagaggcgg tggagtatcc tatgacaccg gaaggacagt ctgcatttat gcagaaggtc
    19741 atggagagga tcacacaggt tccggagggc agaggaaagg gcttctacta ttgggagccg
    19801 gcatggatcc caatgcccgg ttgtggctgg gccaacgagg aatccctgaa ctatattcag
    19861 gatcccggtc cgggcggaaa cgagtgggcc aatcaggcac tgttcgatta tgatggaaat
    19921 gcactgcccg cgttgaaagt gatcagagac tttcagaagt aatgtgatat tcgttcccgc
    19981 aatgcgggga ggatgatata atgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20101 nnnnctttca gaagtaatgt gatattcgtt cccgcaatgc ggggaggatg atataatgaa
    20161 gcaaaatcac ttgcttgcaa gtgtgatttt gcgaaatgta ttgagtgagc tatgctcacg
    20221 atataagatg ccaaatggcg agatggagga aaagatggat aagtttatcg tgaatatcat
    20281 tcaccgtccg gagatggttc ccgagtatgc ggaaaaagta accgggcacg gcaaagaaga
    20341 ggatctgggc agaaaagcac tgctgacaga atctctggat attttcaagt ttcagcagga
    20401 aactgcgcat aagaatggtt tgaagactac cattcagatg acctatgctt ccctgttcaa
    20461 tgacgaagcg gtagcactgg ctaaggcaga tcacgagaag tacggtgatg agatcgcact
    20521 gtctctgtta ggtctgccct gcacagagtt ccgtgagaag tacaagacca aggatttctg
    20581 tatctggatg ttctccatgg aagacaaaaa gtctatcgtg gatgatgtgt tctgcaagtt
    20641 ccatgagaag ttcggattct atcccgaatc caccggatct tactacatgg atgcagatct
    20701 gaccaattat atcaaagcaa cctatcctac cgtaaaatgt gcggtagcaa cctgttggga
    20761 ggaaggcccc aaggcatatc atacctgcaa caactcctgg tataccttca tggatggcgg
    20821 cccctgggca ccttggatcc cttccaagca gaatacccat gcacctgcag ccaacgaagc
    20881 tgaggacagc ggtgtagtag caatccctca cctgtccaga gacctgctgg catgctatga
    20941 cggcaatggc tccaacttcg gaacccatcc tcagaacgta ttgcgtggta tgatctatga
    21001 caccaagacc tgggagtatc cttatctgta caacctgatc gatcagtatc gttccctgga
    21061 gaagtacaac aacggatacg catacaacat gatgttcgta ggacccggct ggctgaacaa
    21121 aatgggccgt tgggagcagc cttacgaact gttgaagaag agttatgaag acggtatgaa
    21181 gtattacggc gacctgaaga aggaaggcaa gcttaccgat atgaccatgg cggagttcgc
    21241 tgattactat cgtcagaaga agacctacac cgagcctgag tgtgctctgt ggagagatat
    21301 tttgtacggt tccgacaagc agttgttctg gtactgtgat cccttcatgc gtgcctgcgt
    21361 aaatatggat cagggcggtg ctatcgtaga cctgcgccct tacgctgcaa agctggagtg
    21421 gccggtaggt atcggcacca agcatgtgac cgatgcttcc tatcctttcc tgatccagga
    21481 gaagtaccgt gcaggttact tcacacatta tgcaggagaa ggtaccgtta gaagtgccaa
    21541 gttaaaacac aacggtgaag aagtggatct gtgcctgtgc cgtaccaaag ctcatttctc
    21601 tcaggaaggc aacaccagaa tcctgacatt ggatcccgta gatatcgagt tctatgatct
    21661 gaccgtaaaa ttacagacca tcgtttcctt cgaggaaggc agctcagcaa tcaagatcga
    21721 gcgtaagatc ctggagatga gcgatccgaa cgcagaagta gaactgaacg agtacatcgt
    21781 agcatgctat ggtaccactg agtactctga ggacatgctg ggtatcaccc tgagcaccaa
    21841 aaagggtgac gaggtagaga ctctggatta cgagtacaag tgccgtgaga tggagaaggc
    21901 agaggctgac gaagtaagag ctgtcattcc tcagatcgag accgcagtat ccatgagcac
    21961 caatgcagaa ggcgctgtcg gatatgtgaa ggaaggatat gcattctccc cgatgtttac
    22021 actgggttac aattccaaaa tcaaggataa ggaggtcgtt gcaacatggc tcaagctgga
    22081 aaaggcaaat taaactacag atgtccctct tgcttcatga gagatatcga tatggatatg
    22141 ttctacgata aggataagaa ggaatactac tgcctgcgtt gtcagtacag gggatccgag
    22201 gaagatgtcc tcaagaaaaa tgaaatggca agattccgtt atggcgctat gtatgagcgt
    22261 tacaccaaat tcgattttga ttgagaaaac ggataaagcg attgcataag agcattgaaa
    22321 aaaggggctg tattacagct cctttttcat atattcatat atgaaaagaa atgaaaaata
    22381 ccttttgtaa aatcaatctg tacaat
//
LOCUS       DAWEJW010000041        22316 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_936_length_22316_cov_53.803782, whole genome shotgun sequence.
ACCESSION   DAWEJW010000041 DAWEJW010000000
VERSION     DAWEJW010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 22316)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22316)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22316
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_936_length_22316_cov_53.803782"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4562..4661
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7822..7921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12948..13047
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6071 a         4622 c         6195 g         5128 t
ORIGIN      
        1 tcttgctatt aagctacaac agtaacgttg gtagcacgga tcttgctgct gttctgagga
       61 tcgcactcgg tatcgaaagt taccttctgg ccctcttcca gagacttgaa accatcagcg
      121 ttgattgcgg aaaagtgtac gaatacttcc tcgccgttag catcgttggt gatgaaacca
      181 taacccttct gagcattgaa ccacttaact gtaccgttat tcattattgg tacctccttt
      241 aataattcag gctttgcctg atccttttga tgattctaaa acgtaggaca aaaaaatcac
      301 atatttttgt aaagcatccg tgaaaaagta gcaatgtctt acaaaaatat gtgagatcaa
      361 tgactttcat ttagaatacg gttagagtat accattgccc tgatcgaaat gtcaataatt
      421 atctttgatt tttagaccta ttttcttatg gaaaaagcat atgattttaa gagcaacagg
      481 atgcggagaa taacgatgga tcttagattt ttgggaaaag tattacaggg ggcactgatc
      541 ggtctgggag cggtgctgcc ggggatctcc ggcggggtat tgagcgtggt gttcggcgtc
      601 taccggccca ttatggaact gctggcggat cctctgcgta agtggaagac gcatctgccg
      661 cgattgctcc cgtatatgat cggttctgcg gcgggatttt taggtgtggc gaatctgctt
      721 tcttatgtgt tggagaccta tccggagcaa tcggtctgtg tatttgtggg gctgatcggc
      781 ggaatgctgc cgtccctgtg gcgggaagcg ggagagcagg gcaggaccgg aggaaacagg
      841 atcgtctccg gagtgacgtt tgcggcaatg atttttttgc tgttttcgtt gcagacttcc
      901 aagactgcag tggagccggg actcggagcg tatctgttct gtggatttgc ccttgctctg
      961 tcggtgatcg ctccggggat gagtttttct acactactga tgcccttagg gctgtataca
     1021 ccgttcgtag aggggatcgc tcatcttcga ccggaggtgt tgattcccgg aatgaccggt
     1081 tgcgtggtga cttttgtgtg tctggccagg ctggtaaacc gcctgcttga aaaacagtat
     1141 gcggggatgt tccacgggat cttcgggatc atcagtgcag ctacagtgat gacggttcca
     1201 tggaacagtt ttgccacatc tccggagacc gcatcccgga atctgttctg catggcggca
     1261 ggaatcgtgg cggcacttct tttggaagtt atgaatgaaa aaatggcatg ttttcccgaa
     1321 aatgagacag aataaggagc gggaacctta tcatggaaaa aggaaggtag aaacatgcag
     1381 atgtatgaca tcgtggcaac gggagtgctg ggactgttgg tcggcttctg gtctggcagg
     1441 agtatccggt ggaaaatgga agaaacagaa gagacagggg aagacaggga agcgagaaaa
     1501 aatacagcag ccaggcaact ggtgagggaa ggtgtcacta taggcagccc ggtgaacgga
     1561 gagatccgta agcgcatgga agagaccgct gaagagaccg ctgatgccgg aactggctgt
     1621 atcagcatcg tgccagagga tggaaaggtc tatgcgccca cggcaggaaa ggtattgaaa
     1681 ctctatccca tgggaaaccg gatccgattc cgcacggaca gcggactgga actgctgttg
     1741 aatatctgca gagacaggga agaactgcac agcgcctatt atcattgcaa tgtcctgcaa
     1801 aacgagatcg tgcgcaaagg aaaactgctg atggaatttg atcaggaggg cctggcaagg
     1861 gaaggcgtgg atacggcggt gacggtggaa atgtgccaga tcccggagcg gggacagatc
     1921 gtcggtacct ggaaagatta tatccgggcg ggagaggaac ttctgtggat acagcgtaag
     1981 ggaagggatc aggaagattc tttctgccta cggtaagctc cgggagtggt gtgataagcg
     2041 gaatgaaagg cccggaaaaa ggtgcgggag cttccgaatc ccacatccag cgcaatctgc
     2101 gtcacggaga gctccgtgga caatagaaga tctctggcat agtccagcct tcgggagttc
     2161 acgtaatcag gaaaagtggt gtggagtttc tcagtgaaga tccgggacag gacaaagcgg
     2221 ctgacaccga attctttcga gagactttcc agggacaatt cttcggtata gtgggcatcc
     2281 agatagagaa gcagtctttg ctccaattcc aggtcatctg tgtttttacg cacttgcaga
     2341 ggcaacgatg gcagcaggtc tgccagcagg agtgtgagat acccttccgc atagacttgt
     2401 acatcggaag gtgtgggacg atccttttcc atggcagcgg ccagtgtttt aagtccctgc
     2461 agggcaatac ggctgtgctc tgacagggag gagacggtaa agtcattgtg ggcaggcttt
     2521 ttgttctgaa aaaactggcg gaaagagtgg cagaaatccg gctcgaagat acagagcaga
     2581 ttctgaccgg gttccacatg cttcaggcca tgcatctggt tcggaaaaac cagaacaccc
     2641 tgaccggggg taagtaaaaa atcttgctgt tccacggtga cggtggtgga accgctcaat
     2701 acatagatca actccaactg tctgtgaaga tggacggcat aggggttgtt tttggataaa
     2761 tataaataaa gctgttcctg acggttctgg taaaagaaac tcaatgggat tccctcctgc
     2821 aacttttgtc actattatga catttatagt cgctaaatgc aagatttgac acaaactaat
     2881 gggaaaatgg cacgcgatac aagggaacat ctgttatact gaaattgttc agaaacaggt
     2941 aactattatc aaatgagtgt tgaacacctg cattcgtaaa taaaaggcgg caaaggctgc
     3001 ggaatggaga aattatggac aagaccaaga tacataaaat gtggatcgcc gatcagggtg
     3061 acggcaccta taccaacccg atcctgtata ccgattattc ggacccggat gccattcgtg
     3121 tgggagagga ttattttatg atcgcttcca gcttctgtaa cacccctgca gtgcctctgc
     3181 tgcattcgaa ggatctggtg aactggaagg tcatcaatta catcattgac aagcttccct
     3241 ttgactatta tgacaagccg gtacacggct gtggtacctg ggcaccggcc atccgttatc
     3301 atgagggaac ttattacgta tttatcccca tgccggacga gggcatcatg atgtgcaaga
     3361 ccacggatcc tttcggcaag tggagcgagc cggcttatgt gcgtaaggta gtgggatgga
     3421 tcgatccttg tcctttctgg gatgaagacg gcaaggctta catggtaacg gctttcgcca
     3481 gaagccgtat cggcttcaag agcatgctgt atatgtctcc catcgaaccg gactgctccg
     3541 gagtgctgga tgacggtcag ttcatctatg atggccatgc gacccagccc accatcgagg
     3601 gaccgaagct gtacaagaga aacggctatt actatatctt cgctccggca gggggcgtga
     3661 agcccggctg gcagaccgta ctgcgttcga aaaatattta cggtccctgg gaagagaaaa
     3721 tcgtcctgca tcagggaaat tctcctgtca acggacctca ccagggtgcc tgggtggata
     3781 ccccggacgg acaggactgg ttcctgcatt tccaggatgt gggcaatgca ggacgtatcg
     3841 ttcatttgca gcctatgcac tgggagaatg actggccggt gatcggtgtg aatgaggtgg
     3901 acggctgcgg cgaaccagta ctgcgctata aaaagccgga agtaggagag acgtacccaa
     3961 tcgatgcacc ggaggacagt gatttcttcg aaggggaaaa cttgggatta cagtggcagt
     4021 ggaatgccaa ttataaaaag gaatggtatg atctggaaaa cggccagctc attcttcatg
     4081 cccaggccgc agacccgaag acgcagctgt gcgatatcag caacctgtta ttgcagaaat
     4141 ggccggcacc ggaattctcc attaccacct gtctgcatct ggaacagatg aaggacggcg
     4201 atgtggcagg actggtatcg ctgggcggat gttatacagc acttgcggtg cagaaaataa
     4261 acggaaagat gagcttacag cagcgcaccg gcaactggac gaaggacgat gaagtgcgca
     4321 ccggtctggg cgaatggaac agtgatgtca tttatatcca gatgaaggtg gagaaggaga
     4381 tttaccgtac attttatgta ggaaccgcag aagaaaattt acagcctgtg ggacagatgg
     4441 tggaagcaac tccgggccgc tgggtcggtg tcaaggacgg actgtttgcc atcaatgagc
     4501 agggtatgga aggcggcctg gtcgctgcgg actatttcgt atatcaggag ctgtaactat
     4561 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggaaggcg gcctggtcgc
     4681 tgcggactat ttcgtatatc aggagctgta actatttata gggcgaagta accacaatga
     4741 gcagagaaca ggccggccgg ttctccaaga aagcctcgta gaggcggttt tgcgaatggg
     4801 gtgctgaata gttactgaat cataaaatta catttctatt tacaatgcaa tgacaatgtg
     4861 atattttggc agttgttaat gtgatgataa aggaacgatg taagatatga cggaagagaa
     4921 aaaaatgtta ttttcacagg atgcactgaa aaaattattg tggccgctga tcatagagca
     4981 gtttttggca attgcaatgg gaatgtcgga cacggtgatg gcggccagct gtggagaagc
     5041 cgtggtatcc ggaatctccc tggtggacag tatctgtgtg ttattgatcg gtctgttcac
     5101 ggccatggcc agcggcggtg cggtcgtggc ggcgcaatat atgggacacg gagataagga
     5161 aatggtaggc cgggcctcca atcagctttt tttagcggtt ggaggtgtgg cccttctgtt
     5221 tatgacgata gcactgatct ggaacaaggg attgctggca ctgctttacg gcaatgtgga
     5281 agcggatgtc atgaatgccg caagaattta tttttatatt gtggcggtgt cctttccttt
     5341 tctgggaatt tataacggtg gtgcggctct gttccgtgcg gtgggagatt ccaaggtgtc
     5401 catgaaagtg tcattggtgg caaatctggt aaatattgcc ggaaatgcga tcctgatcta
     5461 tggtgtaagg atcggtgctg ccggtgctgc gttgtccacg ctgttttcca gaatattgtc
     5521 ggctgtgatg atcatagtgc tgcttggaaa aagtgacaag atcatagtga gcaatcactg
     5581 gaggatcgat acgaaaatct tgggaaagat tctgtatatc ggtgttccca acggactgga
     5641 aaacagtatc ttccagatcg gtaagcttct gaccaccagt ctgatcgccg gttttggaat
     5701 ggctgctacg acggcaaatg ccgtaacggg cagcattgcc agctttcagc agatccctgc
     5761 caacgccatc ggtgtagcaa tgatcactgt gatcggacag tgcatcggtg caggagagac
     5821 agagcaggca ttatattatc tgaaaaaaat gatgaaggcg gcatatgcct gcatgatcct
     5881 gttgggaatt cccatgatta tttttgcaag gccgatctgc ggaatctatc atctgagttc
     5941 ggagacaatg aaaataactg tttttctgtt atgttacagc tgtgtatgtt gtatgctggt
     6001 gcatccgttg tcctttgcac agtccaatgc acttcgcgct gcgggagatg tgagatttac
     6061 catgatcgtg gcgatcgctt ccatgtggct ctgccgtgtg ggcatggcat atatcctggg
     6121 acagggtctg ggacttggcg tcatcggcgt ctggatcgct atgaccatgg actgggtcgt
     6181 acgggcattt ttgttcacta acagggtacg tacgggaaaa tggctgaagt ataaggatcg
     6241 gctggtgaag taaagtaata taatgcaatc tggagaggaa tagaggcgca gtggacaaaa
     6301 atatctgctg cgctaaagtt ttacattgac agattttgca atgtgtttta gcatattttt
     6361 atagaaattg taacggttcc gccaggatcg ttacgcaata atacatagca catcaaagtg
     6421 cagaaatgag gcagaacatg agcaaaaagc cctatgcaga gcgtaatctg aaattcgaga
     6481 cattacagtt acatgtggga caggagcagc ccgatcctgt gaccggtgcc agagccgttc
     6541 ccatttatca gacgtcttct tatgtattcc gcaactgcga tcacgcagca gcccgtttcg
     6601 gactggaaga tcccggtgag atctacggtc gtctgggcaa cccgacacag ggcgtcttcg
     6661 agcagcgtat tgcagcactg gaaggtggta ccgcagctct ggcggtagcc agtggcgcag
     6721 cagctatctg ctacacgatc cagaacctgg caaaacaggg agatcatatc atttctgcaa
     6781 ataatattta cggcggcacc tacaatctgc tggcgcatac tctggtggac tacggtgtga
     6841 ccaccacttt cgtggctcct tccgattatg ataagatcga agcaagcttt caggagaaca
     6901 ccaaggctct ctacatcgag actctgggca atcccaactc ggatgtggta gacatcgaac
     6961 gtctggcaca gatcgcccat gcacacaagg tgcctctggt ggtggacaat acttttgcta
     7021 ctccttatct ggtacgtcct tttgagtacg gtgcagatat cgtagtacat tccgctacga
     7081 aattcatcgg cggacacggc accagcttag gcggtgtgat cgtggaaaat ggtaaatttg
     7141 actgggaggg cagcggcaag ttccctcatc tgtgcactcc gaatcccagc tatcatggtg
     7201 tgactttcac caaggcagca ccgggcgcgg cttttgtgac cgctatccgt gcaatgctcc
     7261 tgcgtgatat gggcggctgc atttcccctg tccatgcttt tattttctta cagggactgg
     7321 agacactgtc tctgcgcgtg gagcgtcatg tggaaaatgc actgaaggtg gtacagtatc
     7381 tgaacaatca tcctcaggta gagcgtgtgc atcatccttc cgtatccacc gatccggagc
     7441 agcaggagtt gtataaaaaa tatttcccga acggaggcgg ttccatcttc accttcgaga
     7501 tcaagggcgg caaggagaca gcgaaaaagt tctgtgataa tctggagctg ttctcgcttt
     7561 tggcaaacgt ggcagatgtg aagtctctgg tcatccatcc ggcttccacc acccatgcac
     7621 agctgtctga ggaagagctg aatgagcagg gaatctacag caatacgatt cgtctgtcta
     7681 tcggtacgga gaatattgat gatatcattg aggatctgga aggcggtttc cagtctgttt
     7741 aaggagagac catgctttca ttagcagttt tctattatgg aaaagcaaaa ccgggaatcc
     7801 cactgcgttc aaggcagtgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 ngttttctat tatggaaaag caaaaccggg aatcccactg cgttcaaggc agtgggattt
     7981 ttcttgtcgg tgccggtgat cgccgacagc cttatttgtc tcccgtgcgc tgctctagcg
     8041 gcatgttttc tgcgttgaat gagaagaccg gcaccaggag ccgcatcgga ctgattcagt
     8101 tctactataa ctgggaagaa gacaagaaat atctattggt ggaaatctac cttgcaaaac
     8161 agaaataagt tcagtataat aggaacgaaa atcaaaaaca gtcagccgaa aggccgcagc
     8221 aatgcggctt ttctaagata cataagttag cgcaagctaa ccatatagct acatgcgaca
     8281 gttagggaaa actaacgtca aaagaaatga tcataatgtg cgaaagtgag agataccaat
     8341 gaaatacaag attgagaaga acaccgtaca ggagacactg atcatccccc tttattcccg
     8401 aaagctatgc tcggagttgt atccgaacct atatcgggac gagatggcgg tacacctgat
     8461 cgaccagatc gactatgact tttcacaggt ggagaaaaaa tctcacagct tgatgcagcg
     8521 tttcggttcg ctggaggttg ccatgcggca gaatgacctt gcgtgggagg tacaaaatta
     8581 tctggcagat catcctgatg cggcggtggt caatttgggt tgtggacttg acagtacggg
     8641 aagagcctgt gacaatggca gctgtaaaat ttacaatctg gattttccgg atgttatcgc
     8701 cgtacgcaat caattactgc ccgccggaga ccgtgaggaa aatatttcct gcaatttgaa
     8761 tgatacggaa tggtttacca aaatcgacgc gtccgaaggg gcgatatttt ttgcctccgg
     8821 agtattctat tattttctga cggagcaagt caggacgctg gtacaggcaa tggctgacag
     8881 ttttcccggt ggtgtgctgg tatttgacgc ggcgaaccgg acagcggtta agatgatagc
     8941 aaagacatgg ctcaggagcg ctaaaattca ggatgtgggt gcgtattttg cggtttcgga
     9001 tgcgaaaagt gagatttccc cgtgggacag cagattgcag gtcacaagcc gtggctatat
     9061 gctgggctat aatgatcttc gagacccttc cgtcagcgga ttattccgct tcctcgccag
     9121 aaccggcgac aaaatcatga agatgcagat cgtaaggatc tgttttggtc agaggtgaac
     9181 tggcaggaat tagaaagtac ctcttgacaa ataccctatg ggggtatatc ttgtagaaga
     9241 aaacgagata cccctgtagg gtattttgcg tatggctgca acgaaggatc cggcagtatg
     9301 ttgcgtgcac tgatatgcag gccggaaaca gcatatgtgt agaaccggga agattctgga
     9361 aaattcggat tccggtgccg gaggctcagg agggggaaaa ttatggcaga acaaaaggaa
     9421 gaaaaaatgg aagaaaaaag catggaatgt tgtcattgtc accagaaggc aacgccccgc
     9481 tccgaaaaag aaagaaaaca attacagaac cgtttaagcc gtatgagcgg acagttaaac
     9541 ggtatcagcc ggatgctgga agagaaccgt tactgcggtg atattctcac acaggtagct
     9601 gccgtggaga gtgctttaca ggcctttggc tatgcaatcc tgaaggaaca tatggagacc
     9661 tgtgtggtgg aagagatccg gaaggggaat actgctgtag tagatgaggc agtggaactt
     9721 gtgaagaaat tgaaataagc ggcggaatgg tcgcagataa acctgtaaat ttgtgctgct
     9781 gcggtgcatt gaaagcaccg gagactttgc aggtcatgga aaggcaaggg tattatgaag
     9841 gaaagatatc gtgttacagg aatgtcctgt tccgcatgtt cttcccatgt ggaaaaggca
     9901 gtaaacaagc tggaaaatgt ggaaaaagca tccgtaaatc tgctgacgga gactatggat
     9961 gtgacctatg acgagacgaa gatcacttcg gcggaaatta tcgatgctgt ggtgaaagca
    10021 ggctacggtg cttcggtaat gaccgaaggc agtgtggcag gtgcaggcgg acagagcact
    10081 tccgggaatg cgggaagtac aggaaagtcc acagccgatg gcaaacagga gctacagcag
    10141 aagctggatg cagacgcaag agccatgaaa tggcgtctgg gaatctccat tggattcctg
    10201 atcccgttaa tgtatgtgtc tatgcaccat atgctgaaag aatggttcgg aattccggtt
    10261 ccggcattta tcgtaaacac gatgcacggc aatgccaatg ccatgaattt tgcattgacg
    10321 cagtttttac tgttattgcc gattctgtat gtgaaccgga agtttttctc tgtaggattc
    10381 aaaacattgg cgcaccgcag tccgaacatg gacagcctga tcgctctggg atccggcgcg
    10441 gcactggtgt acggcatttt cgccatgtac cggatcagct atggcttagg ctacggtgac
    10501 atggcagtgg tagagcatta tgcccacgat ctgtattttg aatcctccgg tacgatcctg
    10561 actctgatca ccgttggaaa atatctggag agccgttcca agggaaagac cagtgaagcc
    10621 atcaccaagc tgatgaatct ggctcccaag gtggcaattc ttgtgaccga ggacggacag
    10681 gaaaaggaag tgcccacgga gagcctgcag aaaggcgata tttttctggt aaagcccgga
    10741 agtctggtac cggtggacgg tatcgtgtta gaaggcaact ccagtgtgga tgaagcggcc
    10801 atcactggtg aaagtgttcc ggtggagaaa caggcaggtg accatgtggt ctccgctacc
    10861 gtgaacaagg cgggcttctt aaaatgtcgt gcggatcgtg ttggcgatga cacaacactg
    10921 gcacagatca tccgtctggt ggaagaggcc agtgccagca aggctcccat tgcacagctg
    10981 gcagataagg tggctggtgt attcgtgccg acggtcatga ccatttccct gatcaccctg
    11041 atcgtgtggc tgataagcgg tgctaccgcg gaatttgcca tttccacagc cattgccgta
    11101 ttggtaattt cctgcccctg tgcgttggga ctggcaacac ccgtggctat catggtggga
    11161 accggcgtag gtgccagcaa tggtatcctg atcaagacgg gtgaggccct gcagcaggcc
    11221 aaagagattg acactgtggt actggacaag accggaacca tcaccagcgg taagttaaag
    11281 gtagaagaag caggcggtta tcagagtgac ttcccgatgg atgccatgat gcagatcgca
    11341 gcagctcttg agaaaaaaag tgagcatccg ctggcggaag ctgtagtgga atgggcagag
    11401 cagttcggtc tggcaattcc cgagatcgtg gatttcaagg caaccttcgg ccgtggcgtg
    11461 gaaggtacac tggcagcaga attccgcgcg gtagaatcag taaaaaatga cggccctacg
    11521 gcggtatttt atgccggaaa acagaacgac accacagatg gctctggcaa gacaacggaa
    11581 cagaaccaga tggcgagaaa tacagtatcg ggtgccagta gcgtgataat tcctgccggt
    11641 acaaaattct atgtaggtaa ccttgccttt ctgcaggaga atcatatcac tctgccggag
    11701 gaagccacag aaggtctgaa ccggatggcg gacgaaggca tgacaccgct ccttgtggca
    11761 caggaaggaa gattcttagg agtcatcggc gtggcggata ctgttaaggc cacttcttat
    11821 gaagctattc gtgtctggga gaaaatgggt gtcaaagtca tcatgctgac cggagacaac
    11881 cgccgtactg cagaagctgt acgacagaag ctgggtatct ctgaggtcgt ggcagaagta
    11941 ctgccgcagg ataaggagaa aaaggtcagc gaactgaaac gtcagggcca taaggtagcc
    12001 atgatcggtg acggcatcaa cgatgccccc gcactggcag ccgcagatgt aggcatggct
    12061 attggtgcag gcacggatgt agccatggag agcgcagata ttattctgat gaaaaatgat
    12121 ctgcgggatg ccgtgacggc catgaaactg agccgttccg tgatccgcaa catcaaggag
    12181 aatctgttct gggctttctt ctacaactgc atcggcatcc ctctggcagc cggtgtgctg
    12241 tacccgtctt ttggaatccg tctgaatccc atgttcggcg cggcagccat gagtttaagc
    12301 agtatctttg tcgtgggaaa tgccctgcgg ctgagaggct ttaagagcgg atttactccg
    12361 ctgaaaacag gaacgccgga agcagcccgg acggaacagg agacaacgca ggcagctgtg
    12421 gagggagaac agtgcatact ggaagccccc gcagagcaaa atagcaaaga agagaaagag
    12481 aggacaaaca caatgacaaa agtaatcagt atcgaaggaa tgatgtgcag ccactgcacc
    12541 gggaccgtac agaaggcact ggaggccgta gagggcgtaa agaccgtaac catgagcctg
    12601 gagcagaaga atgctactgt ggaactggct tctgatgtgg cagacgaagt actgaccaag
    12661 gctgtagttg acgcaggata tgaagtaaag ggcgttaccg aaaaataaag tgttactgta
    12721 ggttacaata acggaaaaag agaaatggcg ctgccaagat gccgtgtttt tgcaaacacg
    12781 acaccggacg gcgctatttt gggtctatag agaaaaaagt gatccgcagg tacttttcaa
    12841 tgcaatattt caggaatata tgtggcgtat gggtctatac agaaaaattt caggcataga
    12901 tacttaaatt gtgaaaaatg agtatctgga gagtgatttt tctcctannn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnngcg tgaatggaaa ataattttcg aaagattcca
    13081 tataaagttt ataatttctt aattgatttt ctctgactat gtgttatatt aaatatagaa
    13141 cagatgagac agaaagcctg tgaatctgtt cgcaagtaag aaaataaata aagcaactat
    13201 tcagagcctc attcgcaaaa cgctgttaca cagtttcctt tttgctcatt gaggctacct
    13261 cgccaaataa atattgccaa agctttttat gaatgcaagc aatcaacaaa gctcaggcaa
    13321 tatttaatgg ctctgaatag ttgcaaattg cataacctta tgtgaggagg cgaacgatta
    13381 tgaatattga taaatttaca cagaaatcca tagaagcagt caatggctgt gaaaagctcg
    13441 ccatggaata tggcaatcag gagatcgaac aggaacatct gctgtacagt ctgctgacgc
    13501 tggatgacag tctgatctta aagcttgtgg aaaagatgga gatccagaag gatcattttg
    13561 taaaccgtgc aaaacaggct ttggagaaga gagtaaaggt gcagggcggt cagccttatg
    13621 tcggtcagta tctgaacaag gtactggtca gcgcatcgga cgaagcaaaa cagatgggtg
    13681 acgaatatgt ctccgtagag catctgttcc ttgccatgat caagaatccc aacaaggaga
    13741 tcgccgagat ctggaaggaa tacggcatca ccagagaacg tttcctgcag gcattgtcta
    13801 ccgtaagagg caaccagaga gtcaccagtg acaacccgga agcaacctat gatacattgg
    13861 aaaaatacgg gcaggatctg gtggagaaag caaggaacca gaagctggat cctgtcatcg
    13921 gacgtgacag cgagatccgt aatgtggtac gtatcctgtc ccgtaagaca aagaataacc
    13981 ccgtgctgat cggtgaaccc ggtgtcggta agaccgccgt ggtggagggg ctggcacagc
    14041 gtatcgtcaa gggcgatgtc cctcagggat tgaaggataa gaagatcttc gcactggata
    14101 tgggcgcact ggtagccggt gccaaataca gaggtgaatt cgaggaacgt ctgaaggccg
    14161 tactggatga tgtgaagaac agtgacggac agatcattct gttcatcgat gaactgcata
    14221 ccattgtcgg tgccggcaag accgacggcg cattggatgc gggcaatatg ttgaagccca
    14281 tgctggcccg tggtgaactg cactgtatcg gtgccaccac actggatgaa tacagacagt
    14341 atattgaaaa agatgccgct ctggaacgtc gtttccagcc ggttatggta gaagaaccca
    14401 ccgtggagga tacgatctct atcctgcgtg gactgaagga tcgttacgaa gtatatcacg
    14461 gtgtcaagat catggataac gcactggtaa gtgcagccgt gctttccaac cgttatatca
    14521 ccgacagatt tctgccggat aaggccattg acctggtgga tgaggcctgc gccctgatca
    14581 agaccgagat ggacagtatg cctacggagc tggatgaact gcagcgtaag gtgacacagc
    14641 tggagatcga ggagaccgct ctgaaaaaag aggatgaccg gatcagtaag gaacgtctgg
    14701 cagatctgca gaaggaactg gcagagttaa aggatcagtt caacactaaa aaggcacagt
    14761 gggataacga gaaggcatcc gtggataaac tttccaaact gcgggaagag agagaccgca
    14821 tgaataatga gatccagatc gcagagcggg aaggagacta tgaaaaggcc ggcagattaa
    14881 aatacagcga actgcccgca ctggagaagc agctggcaca ggaagaggat aaggtaggag
    14941 aatccgatct gtctctggta catgaaaaag tatcggagga agagatcgca aggatcattt
    15001 cccgctggac cggcattccg gtagccaaac tgaccgagag tgagcgtaac aagaccctcc
    15061 atctggacga agaactgcac aaacgtgtgg tgggtcagga cgagggtgtg accaaagtca
    15121 ccgaagccat catccgttcc aaagccggga tcaaggatcc cagcaagccc atcggctcct
    15181 tcctgttcct gggacctacc ggtgtcggta agacggagct ggccaagagt ctggcggcag
    15241 cactgttcga cgatgagaat aatatggtac gtatcgatat gagtgagtac atggagaagt
    15301 attctgtctc tcgtctgatc ggagcgcctc ccggatatgt aggatatgag gaaggtggtc
    15361 agctgaccga agctgtccgg agaaaacctt acagcgtggt actgttcgat gagatcgaga
    15421 aggcacatcc cgatgtattc aatgtactgt tacaggtact ggatgacggc cgtatcacgg
    15481 attctcaggg tcgtaccgta gactttaaga ataccatact gatcatgacc tccaacatcg
    15541 gcgcaagcta tctgctggac ggcatcaacg aagacggcac catcaagccg gaggcagagg
    15601 cggcagtcat gaacgacctg cgggctcatt tccgtccgga atttctgaac cgtctggatg
    15661 agacgatcct gttcaagcct ctgaccaagg acaatatcgg cggcatcatt catctgatca
    15721 ttgccgatct gaacagacgt ctggaggata agcagctgac catccgtctg tcggaggaag
    15781 ctatgcagta tatcgtggac aatgcttatg atccggtcta cggtgcacgg cctttgaagc
    15841 gttatatcca gaagtacgtg gagaccctgg cagccaagat gattctggca gatcaggtag
    15901 aagaaggcga taccattgtc attaccgtgg agaatgatca attggccgcc aagtgcgaaa
    15961 aaggatcgaa aaatcaataa tctgtaaaat aaaacaggag caaagatgat gatacttgtc
    16021 atttttgctc ctttcgttta caatataact ctgtcaaaaa caggaaaaga gaggaaaacg
    16081 ttatgatacc acaggatccc gtaatgttat taagctttat aaatttaaaa ctccgtgatt
    16141 tctacggaag cctgaaacag ctctgcgatg atctggatgt gaaggaacag gagatcacag
    16201 ataaactggc agccattgac tatcattacg atgaagagag gaatcagttt gtataatacg
    16261 agcagtacca atcccacagg aaaaaaggga accagatgcc tgatctgtac cggttgtggc
    16321 agatgtccag gagtgatccg ggggatgcag gtggtgacgg aaaagctgga acttcctccg
    16381 ttaacattac aaaatccgga cggtaaacgg ctgatgactg tggatattgg taccactacc
    16441 attgccatgc agttgtatga catgaccggt aaagtggtgg acagctttcc cagtgtgaat
    16501 ccccaggtca gctatggtgc cgatgtgctc tcacggatag aggcagcgaa ggatccggga
    16561 aaagcggcag atatgcagaa aaaagtccgt gacctcatgg aaaaaggagt gcagcgtttt
    16621 tccaaaaagc tgcagccgca ggagactctg cagatggtcg ttgcggcaaa taccaccatg
    16681 acctatctgt tcatgggctg gaatccggag gaactgggca aagcaccttt tacagtcagt
    16741 catagcggcg cagtggagac agaaattgca ggagtggcct gtcatatcat accgggattg
    16801 tcagcctttg taggcggcga tattactgcc ggaatattgg ccagtggaat gctcgaacag
    16861 gaggcctcca ccctgctcat cgatcttgga accaacgggg agatggcact gggaaaccgg
    16921 cacagattac atgcctgtgc cacggcggct ggccctgctt tcgaaggcgg agccaaccgg
    16981 ggcatctggg gcgcggatat ggtgagactg ttacaaaaac ttctggaaga ggggctgatg
    17041 gacggacagg gtcttttgaa ggaaccttat tttacgaaag gagtccgtat cggcgatgtg
    17101 ctggtgacga aggagtccgt gcgtgcagtg cagctggcaa aaggagccat tgccgcaggc
    17161 attgagattc tggcggacag ctatggcata agattttcgg acatttcgaa agtggtgctg
    17221 gcgggaggat tcggttatta tctggatccg aaggcggctg tggctatcgg gctgcttccg
    17281 gaagaactgg cagattgcac ggtgaccggc ggcaatacgg cactgagcgg tgcagccatg
    17341 gtgggcagtc ggatccttac agcaggtgga aagcaaacgg cggaaggaac attgccagat
    17401 ggtggggatg accttcgaaa acggcaggaa ttgcagatac gtatcctaaa tctggcggag
    17461 gaagagaagt tccctgagac ctttctgacg aagataaatt ttaactaaaa atatacaaaa
    17521 agcataacaa atggcagaaa atgttgttcc attctggtaa ttattatgct ataattttaa
    17581 atatctataa caagtaccgg agggtattaa atgagtacga atgttgaaaa accaaagaaa
    17641 tctcttttta cggggaaaaa agagacgact tccgaaaaga agccgaagcc tgaaaaggca
    17701 gtgaagggaa agaaggcatc tgcggagaag aagcaaaagg ctccgaagca gaaaaagagt
    17761 ggaaagagct cgaaactgct cagcatccgc aacaagatcg ttgtatgttt cctggtgcct
    17821 attgtgttta tggtgatcat cggtgtgtca gcataccaga aatccgcaga aggattaagc
    17881 gagaaattca ccgattccac attacagacg atgcgtatgg ccacggaaaa tctgaatatg
    17941 agctgtgatt ttatccgctc agagggtctg aaatatgctt acgatgatga tctgcggaaa
    18001 tatttcctgg gaatgttcga agataatccc gtggataagt tgaatttcct gacatctacg
    18061 aagtccaacc ttctgtcggt gcagacctcc aacccgttca tcagccacat gcacattatt
    18121 cccaagaagg gagtaaacct tctgtccact aagctttcat ccggtgtgga cggtttcctg
    18181 gatgagtaca aggaagacgt ggcaagcggg gaaggcagaa gaagtatccc gcagtggatc
    18241 gacagtcatc ctgtactgga tgaaaaggta acggagaccg aaaaagatta tattatggcc
    18301 ttcgaaatga tgagccagtc gaacaatgcc tgcgttgtca tcgatatcaa gccttctgct
    18361 attacggatt tcatggaaga gatcgatatc ggagacggca gtatcatcgg atttgtaact
    18421 cccggaggca gagaactggt tgttgagaac gtagaagaag gaaaagaaag tgtccttccg
    18481 gaagagggaa atgtattctc cggacaggag tattatacag ctgtcatgga tgaggcagta
    18541 gcggatgcag gtactgcgga ggtagatttc cagggtgaga agtatctttt catttacagc
    18601 cacagtgcag atatcggctt taccacctgt gcactggtac ctatgagagt agtgaccagt
    18661 caggctaccg agatccgcaa catgaccatc ggtctggtga tcctggcatg tgtgatcgta
    18721 gtgatcgtag gtatctttat cactgcaggt attgagaata acatgagacg catttccaga
    18781 aagttcggtg atgtggcgaa gggtgatctg acggttaccg tatctgccaa gggacatgat
    18841 gagttccagg atctggcagg ctcggctacc aacatgatca cgaatacgaa gaagctggtc
    18901 aatcaggtaa gcaatgctac cggagagctg gaagtgtctg cacagaacgt gggacaggct
    18961 tctgaactga ttcatgaata ttcacaggat attacccgtg ccatcggtga gatcaacgaa
    19021 ggtatggaag aacagtccag acatgcacag gagtgcgtgg agaagaccga tatcctctcc
    19081 aatgagatcc aggaagtcag ccatgtggta gaacgtgtgg agaagctggt aggcgagacg
    19141 gaaggcatga tcaacaaggg aatggagatc gtacaggtac tgggtgatcg tgctggcgag
    19201 acaacacaga tgactgtgaa agtgagtgag agtatcgaat ctcttcgcaa ggaatctgag
    19261 atcatcaaca gtttcgtagc tacgatcaca gagatcacag agcagaccaa cctgctgtca
    19321 ttgaatgctt ctatcgaggc agccagagcg ggagaggctg gacgaggctt cgctgtagta
    19381 gcagaagaga tccgtaagct ggcagatgat tctgcaaagg cagccggcga gatccgcaac
    19441 aatgtggcca acattacagc ccagacccag aacagcgtgg agagtgcaag ccaggcgaaa
    19501 gcaatggtag aactccagac caaagctgtg gaagaagtga tcgcagtatt ccgcgagatg
    19561 caggagcgta tgggccagct gatcgaggga ctgaaagata tcgtggtaag cacggagaag
    19621 gcagacggtg agcgtagcgc tgctgtggca gccgtgaaga atatctccga tatcattgaa
    19681 gagaccgcag gcagtgccga gaccgtcaac gatgtggcca ataagcttct gaaccatgtg
    19741 gagaagctga gcactactgc aagtgtactg gatgagaata tggaaggact taagaacgag
    19801 atttccgtat tcaagatctg agtataaata aaaaagtaac atacagagga gatccgggag
    19861 atgagagtct cgggtctcct tttttgcaag catgttttat tgcggcagca ggaatggtca
    19921 cttttgagaa tgcatatatt ttctgtgaaa gcgtgaaacg gacggcttga aatgaaaaaa
    19981 gtactggcgg gaattctgat ctttgacatg cttctgggaa tcttgtgtct ggcagtggga
    20041 acggaacgat acgcagagca gaagtcatat ggaacctata tagaaacaga caccggcgtg
    20101 tgcgggacat caggagaacc gaaaaagatc gcgctgacct ttgacgacgg gccgcatccg
    20161 aagtatacgg aacagcttct ggatggattg aaggagcggg gagtggtagc tacttttttc
    20221 gtgacgggag agaatgcgga gaattacccg gacattatcc ggagagaaca ggacgaagga
    20281 catctgatcg ggaaccatac ctatagtcac atacagctca cttcccgcaa ccgggagacc
    20341 tttcgggagg agctggtgca gaccaatgaa attctcgaag aaatcacagg ggagaaaata
    20401 agctttgtcc ggccgcctta cggcagttgg gacaaaagct ttgagaaaga actgaatatg
    20461 ttcccggttc tgtggaatat cgatcctctg gactggtgtt cccacaatgc agactgtatt
    20521 gcgtccaaag tggtggagaa ggcgggggac ggcgatatta ttctgatgca tgattattat
    20581 gagacttctg tcaccgcagc actggaagta gtggatattc tgcagaaaag aggctttcag
    20641 ttcgtaacgg tggaggaaat cctgtttgac tgaatcattg taaaacaaga gccacctgag
    20701 tgataaaatt cgccggatcc ttatgctggg gaggaccttc cagataagta tgccgtaccg
    20761 tgcctgacag agacagattc tgcgtagctg cgtaggcaga cagttctcta cgggcatccg
    20821 ggagtgattc gtaacagccg tgatagaaga tgccgagagc tttctgatcc ggcagatgca
    20881 ccacatgttc tcccttgctg gcaggaggta cggagataca acaggtgaat tcttccggat
    20941 gttccagatt gtactcaata aaaaacatcc gcttactgct gtcggagccg tacagatgaa
    21001 tggcttccat gaagacctcc cgcaggaatt ctttgcgctc ttccactccg ttaaaagccc
    21061 ccttgcggca gtacaccgtc tgggcgggga gggtaagaaa ctgtatcatg gaagcagccg
    21121 gggacgcagt acgttgcttt aacttctcga tactctgcgt caggtcgtct cgtaaggctt
    21181 ccagacgttc gatcaatggt tccagatccg tggatccatt ataataggag gagatctcat
    21241 ccagggacag ccctaaattc tgcagtaccc ggatggagcg gatcagggag atggaatccg
    21301 gggtataata gcgattcccg gcagttcctt cttttttatc cggaagcagg agacctttgg
    21361 cttcgtagtt caatatgatt cttctggtga tgccgatcgc tttggagact tctccgatgg
    21421 acatcagtct tttttcactt tttccgtttt cttcgtttga tgacatcaaa aatatgcctt
    21481 tctcaaaatt atccttgcat cgtacatcga tgtacggata taattattgt aataagcgta
    21541 gaaaaacatg tcaagacaca tacgataaaa cgaatgagct gcatcggttt gtgcggcaaa
    21601 ggaagaatca tgataaaagt agctttttgt gatgacgatc tgtcagtact taaggaatta
    21661 agcgttctga tggacagata tcgggtagaa cggaatcagg atattactta tgcagcattt
    21721 cacagtccgc tggaattgct gtcggaagta gagcggggga cccggctgga tattatattt
    21781 ctggatgttc tgatgccagg acaggatgga atcagtgccg ccagagagat ccgtaagtat
    21841 gatacttcgg tgaagatcat ttatctgaca tcctcttcgg aatacgctgt ggattcctat
    21901 gaagtggggg cctattttta tcagttgaag cctatctggc aggagagctt ctaccgattg
    21961 atggattcgg tactcaggga gtgccgcaag aatgaaaaga acagtctgat cctgcgatgt
    22021 aagacgggaa tcacaaggct ggaactggac aaactgaagt actgcgaggt aacggggagg
    22081 aatctgctgc ttcacaaaga agacggagaa gtactggaca gcgttggtaa actggacgat
    22141 ttgtgcgaac agttgaagga ttatgaatgc tttgtccgtt gccacaggtc atatatgatc
    22201 aatatggatt atgtaaagag catatctaac aaagctgtga tcctgtcgga cggtacccag
    22261 atcccgattc cgcatggaaa atattcggaa ctgaaagaca aatttttttc ctattt
//
LOCUS       DAWEJW010000042        22015 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_951_length_22015_cov_62.768898, whole genome shotgun sequence.
ACCESSION   DAWEJW010000042 DAWEJW010000000
VERSION     DAWEJW010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 22015)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22015)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22015
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_951_length_22015_cov_62.768898"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    13577..13676
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5070 a         6119 c         4641 g         6085 t
ORIGIN      
        1 tcacatccgg agaccttaca tcgagacatg tgtatcgcat gctccgggag ttcgtctggt
       61 ctaaggctcc ctccgcaatg agccacgcta ttcataagat tgtcagcaaa gaacgctgtc
      121 aatcttattt catagacggc tgtcgccgta taaaaataga gtgaaccagc ctcgcttgtg
      181 cgcaagcgcc catcgaggtg ttccactcta tttttgcatg gctcgtagct atgctaactc
      241 tcaatttact taaagtactg taccccggat tcaaagatct tcatatcctg ctcgccatag
      301 atgttcatgg ccacagcggt atctcttctc tcggagtgtg ccatcttacc gaggcatctg
      361 ccatcggggg atgtgatacc ctcgatcgca cagtaggaac cgttgacatt gtattcctca
      421 tccatggata cattaccgtt ctgatcagcg tactgagtag cgacctgtcc gttagcaaag
      481 agcttgtcga tccactcttt cggtgctacg aaacggccct caccgtggga tgcgggattg
      541 cagtaggtag cacccagtgt agcgccctgt aaccagggag acttgttgga tacgactttg
      601 gtgtatacca tcttggagat atgacggttg atggtattga aggtcagggt aggtgaatcc
      661 tccttctgac cggtgatttc gccgtaagga accagtccca gcttgatcag agcctgaaat
      721 ccgttacaga tacccagtgc cagtccgtct ctctcattta acagcttcat gaccgcttcc
      781 ttcagcttct cattctggaa tgctgttgca aaaaacttcg cgctgccgtc aggctcatca
      841 cctgcggaaa agccgccggg gaacatgatg atctgtgcct gtgcaatgga cttctcgaat
      901 tcctctacgg attcgcggat atcctctgcg gtcatgttac ggaacactct tgtctccaca
      961 tgggctccgg cacgttcaaa cgccttggtg ctgtcgtatt cacagttggt tccggggaat
     1021 accggaatga atacggtagg ctttgcgatc ttgtgcttac atacataaat actgtctgcc
     1081 ttgtaaacat tgcttgccac tgcctcggta cccttcactg ccttggtggg gaataccttc
     1141 tccagcttgg aagtccagga aacaagtgcc tcttccatgg tgatcttcac atcaccatat
     1201 acgaatacag gctcctcggt cacggtaccg atcacggtgt aatccagttc cttctcttcc
     1261 agtgcagcca tcttgtctgc ggggatctct gccagaatat cgcccagtcc gttgtcaaac
     1321 aggtcgtctg tggttacctc atcgtcgatc ttcacaccca gcttgttacc gaaggccatc
     1381 tttgctacag ctgctgccac acccttggaa tccagagcat aagcggatac gatcgtacca
     1441 ttttcggtca gttcgtggat cttgccatat aattctgcca cattctcata catcggaatg
     1501 tcgaaatcgt ctttattaat agaaaaacgt accagcttat tgccgggggt cttcagttcc
     1561 ggagtaataa tatcgccata ttttgccacg tccactgcaa aagataccag tgtgggaggt
     1621 acatcaatat cattgaaagt accggacata ctgtccttac caccgatgga gggcagcccg
     1681 aacccgatct gtgcatcgta ggcgcctaac agtgctgcga aaggctggct ccaacgctca
     1741 ggatcgctgg tcattctgcg gaagtactcc tggaaggtaa agcggatctt gctgaaatca
     1801 ccgccggaag ccacgatttt cgccatggac tctgtcactg cgtagacagc tccgtggtaa
     1861 ggactccagg aggacagata cggatcgaag ccgtaactca tcatggtcac ggtatcggtc
     1921 ttaccttcca gtacaggcag tttggctacc atggtctggg tctcagtcag ctgatatttt
     1981 ccaccgtagg gcatcaatac actgccggca ccaatggagg agtcgaacat ctctaccaga
     2041 cccttctggg aacatacatt gaggtcattt aacagtgcca cccaggcacc cttcacatcg
     2101 ccctcttcca gtttctcacc tacagcggga acagcccatt tatcaaaata attctcttct
     2161 tcggaaggaa tatctacttt tactttggtc tcctgatggg caccgttggt atccaggaaa
     2221 gctctcttca gatccacgat gggcttgcct ctccagttca gtaccagtct gggttcctca
     2281 gtaacaactg ctacgggaac agcctccagg ttctcttcct ttgcatagcc cagaaaagta
     2341 tctacatcct taggatctac taccactgcc atacgctcct gagactcgga gattgccagt
     2401 tccgtgccat ccagtccggc atatttcttg ggtaccttgt ccagatctac ggtcagaccg
     2461 tctgccagct caccgatggc aacggagaca ccgccggcac caaagtcgtt acattttttg
     2521 atcagacggc tgacttcctc acgacggaac agtctctgga tcttacgctc ggtcggtgca
     2581 ttacccttct gtacctcagc accacaggtc tcgatggaag tatcggtatg taccttggag
     2641 gatccggtcg caccgccgca gccgtcacgt ccagtacgtc cgcctaacag gatgatgata
     2701 tcgccgggat cagaggtctc acggataaca ttcctgcggg gagctgcgcc cataacggca
     2761 ccgatctcca tacgctttgc cacataagaa ggatgataga tctcttttac aaagccggta
     2821 gccagaccga tctggttacc ataagaggaa tagccactgg ctgcgcctct taccagcttc
     2881 ttctgggaga gcttgccgtg caaagtctcg gatacaggaa cggtaggatc cgcagcaccg
     2941 gtgacacgca ttgcctgata tacatagcca cggccggaca ggggatcacg gatggcaccg
     3001 cccagacagg tggcagcacc accgaaaggc tcgatctctg taggatggtt gtgggtctcg
     3061 tttttgaaaa ataccagcca ctcctcggtc tctctatggt cgccgtaatc catctccaca
     3121 ggaaccacta cggagcaggc attgatctca tcggattctt ccatgtcatc cagtttgccg
     3181 tcttttttca gctttctcat ggccatcagt gccagatcca tcaggcagac gaacttgtcc
     3241 tctctgcctt tgaagatctc ttctctggta tccagatagc tctggtaagt ggtctctaag
     3301 ggagaacgat aataaccctc accgaattcc acatcggtca gctcggtgga aaaagtggtg
     3361 tgacggcagt gatcggacca ataggtatcc agtacacgga tctccgtcat agaaggatct
     3421 ctcttctcat catcatggaa atatttctgg atatgcagga aatctttgaa ggtcatggca
     3481 agtcccagag attcatacag agcttttaaa ctgctctcgt ccatatctct gaagccgtca
     3541 aaaacagcaa catcagcggg atcatcatac accgtgatca gagtatccgg cttctccatg
     3601 ccggtctctc tggaatctac agggttgata cagtaagatt tgatcttgga aaattcttcg
     3661 tcggacacct gtccttcgat catataagtc acagcggtac ggatcacagg ttcttcgtcc
     3721 tcttttaaga acttcacaca ctgcactgcg gaatccgctc tctgatcgaa ctgtccggga
     3781 aggaactcta cggaaaatac ttttgcatcc gcaggaatct cgatggtctc ttcatacaga
     3841 tcatctacgg gaggctcgga aaatacagtc ttgcaggctc ttgcaaacac ctcatccgaa
     3901 atattctcca catcatagcg gataaactca cgaacctttg ccacatcgat acccagataa
     3961 tttttcagtt cgtcgtgcag ctcttttgcc ttgacggcat aaggttcctt tttctctaca
     4021 tagatacgtc ttacgttgct catgtctctc ctccgaaatt ttatactatg cgacactatt
     4081 cagaacccca ttcgcaaaac cacatcttgc gatgcttact ttttgctcat gtggttatct
     4141 caccatataa atatcatcag atgcccttac aatgcgagcc tttgacgtac atatgattat
     4201 atttacatgg ttctgaatag tatcatacaa catactatat tttcccacaa tagcagaaaa
     4261 caacaaattt agatagattt tacctgcctt tttgaacggt tcgtcattca tgcagtctgt
     4321 ggatcagccg gtaagcgatg tccttctcgc agatcagggc ctccgtgtgg gcgaaaaata
     4381 cgatgccgtc cgtgggggcc agaatcttct ccctcaccgt tccatcgtaa gggtctataa
     4441 tttctgccat ctcatttcca tagcgcacat cgtctcccgc atgtactttt ccccggaaaa
     4501 ttcccgcccg gttcgcaggt acatcactca gatccttttc atatataaca tgactgatat
     4561 agccggaatg actctcgtac cggatcagcc ccatacgttt caggaaacgt aagaccgctg
     4621 ccaccgcctg tcttgcactc acctcatcga tctgggtatt ctgattcgtg tataccgaaa
     4681 aggcggccgt catctcatcc tgccagttat aattcagtgt cttggtatca atgggtaccg
     4741 gcttgcgcac caccacataa ggaagtccga acagattcgc caacgacgta ttctgatagc
     4801 ctgtctccat catacgcaca tggggcacga attctcccgg tctgtaaaag gacgtaaact
     4861 ggattccata aacgtaatcc cggatctccg taagcaacgc attggtcaga cggtccgccg
     4921 gctccccgta atcatttccc ggaaaactcc gattgatatc cgcttccttg acaccgaaaa
     4981 agcggcggct gatattcacg gaataggcat tgatggtagg aatcaccaga atctgtttgc
     5041 cggcactgat acagccgttg ttttccagtt cccgcagtgc cttcaccagt tgggaacaaa
     5101 tatacatctg ctggatctca ttgcctctgg ctgcgcccac gatgcaggca gacttctcgc
     5161 ccttgccgaa ctgaaagccc ttgatgcgca tatcttctct gtaggtcgaa tgtatggtat
     5221 aaatatctct ctcaaacata atcttttcac tccgtcagga tccgcgccag cagatctcct
     5281 tcatagacca ccggataagc tctcagggta aacaacagcc cgccttttgc ggtacggatc
     5341 tgctgcttca ccgtcccttc aaatggatcc accacttccc cgatcagatc tccttttttc
     5401 acatagtggt tgtgctcgat cagcggcagg aacacaccgt ctgacttgct gcggataaac
     5461 tccacctcac cgtcactgga tatcgcaggc tcctgcacct ttggctccgg gcctgtccag
     5521 atccccattt cactcatgat gtggaaaata ccgtccacga tctgattacc gaaggatctg
     5581 tggatgtcat gcccctgtcc catctccagc accagtgtgg gaactcccag cagattcatg
     5641 gaatgggcca gcgtcgattc atgcaccgtg gcagtggcat tcatccagat catgtcggca
     5701 ttggtcattt tcgcataggg aagcagtgcc tccgcaaaat cttcactgag tcgtacctgt
     5761 gggatctcac gcacaaaaat atcactggcg tgcagatcga tacaaatatc ggatccgatc
     5821 agggaatcca ccacagttgc cgtgatccgc tccatcatat cccccgatgc gtctcccgga
     5881 aacatacggt tcatatccat atccagtctc ggcaccgtcc ggctagccat gtcgattccc
     5941 agcggattca atgccggata gatatctacg attcctttca aatactccgg atgctctttt
     6001 actcttcgcg ccgtctcata acagatgtac tgtccctcca gttcatcccc gtggatgccg
     6061 gacaccagac tgattctgga taaattccgt tcttctccct ccggacggat ccggttcctg
     6121 cggattgcca gatgtcctcc cgccggaagt tccatgctga tgatcgtttc tgtcatactt
     6181 accttttccc tctctttatc tctttgcagc ttttctacac agaaaatgct tcaggaaact
     6241 ttccctgaag catcatcacc cgttgtctta ttaatcttct acacccaatc cgcgcaacag
     6301 atacaggatc tcacgatcca ccgcttccgc cgtcacgccg atggtctggg ccgaaatctt
     6361 cagtccttcc agcacaaaca taatatttct cgcagttccc tcacagtcct cgcagataaa
     6421 ctctccgtta tccacacctg cctcgatcag tttttcaata atcttcactg cggaatcaaa
     6481 ctgctttttt aaaatattgt ctctcttggg cagttcgttt tcaaaataga attcataggt
     6541 agcctgcgtc agtgtattct tcttgcgcag aagctctttc ttctgctcct ttaaaaacag
     6601 aagcaggata tccgctgcgg tagcatcctc cgtaatactg tcggaaaata cgtcatccgc
     6661 ctcctcactc tccatgcgca gcacttccat gaagatctga ctggtattct caaaatataa
     6721 atagagtccg ccacggctga tgtcgcaggc ctctacaata tccttcatcg ttactttttt
     6781 gaagcccttc tccacaaata cttctctggc tttctctacg atatatcgct ttttctgtac
     6841 tgatttttct cccatggatc tatcctgcct ttccccactc aaagctttcc gttcgccggt
     6901 gtcttatccc agaattcaat gatctcctgc atctgtttca gtaccagata aaagatctgc
     6961 tccgataccg cctcgctcca cagatttccc tttgttctgc cgcctaagac atattttgac
     7021 aggattcctc tgaggatatc ccattccttc catcctgctt tctgaataat atcccgctcc
     7081 tcctgatgag tgcggatccg gtgtcttgtg tatacatagg catagttgcg atccatcagg
     7141 ggacttttcg tcgcttccct gacgaaattc aggagagtcc cgtcatatac cggaaaagac
     7201 acggaattca ctccgatatc ctgtccgctg tagatagagc tggctctctt gccggcgctc
     7261 tgctccagcc agggcaggta gggaagtaac ggcctcactg cctgcttata tgattccagc
     7321 aactgctctc tgtattcact gctctgttcc ataaccccaa tatacctttc catgtaccgt
     7381 tttctaccga aaacatcgct tttttcgtct gtcaatgtaa aagtgacaca tatgtagttt
     7441 tattttatca catttctatc aaaagtacag tagaaaattg ttggtattat ggtgtataat
     7501 gtgtattaaa tatttgtatg ataagacgag gtattggaat ggttggaatc gtattggaga
     7561 aggggaaaac tatatatagc tatgggcagc ccatgactgc attgcatctg attaccaacg
     7621 gtaaagtaga tgtatcatat cccggcggct cttatactct gggaaaggga gacgtgatca
     7681 gtatctgtga gatctgctcc gagatccatc tgctgggcta taccacgttg gaggatacaa
     7741 cgatcctgac gtatcccctg accagtctgg attccctgaa tgatattcta cagaagcacc
     7801 cggacgtagc acgtctgttc tgtctgtcct gtttccgtca gatcaatatt ctgctgaacc
     7861 gcagttccat ttcggaattg aactgcagtg agctgtatcg tactctgaca gatgatattg
     7921 ccacctacaa tacactgtgt agccgttacc gtctgcaggc gagatccctg gaacatttcg
     7981 atgagctgaa tgcttttctt ggggatgatt caccggacat ctggctgaac ggctattaca
     8041 tgggcttaag caggatcctg gcttcggaca attaccggat cctggtacag gagtcggatc
     8101 tgtccattgg tatgctgcgt aagggaagtc tggacttccg ccgtacttac cagagcctgg
     8161 aggaacagtt ccactatctg cagcaggtcg gttccttcta tttccgggaa tccggcaacg
     8221 atctttttga tttttatact tctttgttct ataaactggg acaggataat gaagacagca
     8281 aggcggtcta cgaccgcatt caccgtatgc tttctaaggc cggcgatctg tccttcatcg
     8341 atcaggaact gtttgcctcc agatcccaga ccttccagag cagcctgaat ctcatggaat
     8401 ccaatgattc cgctgaggca ggttcttccg gagattccga gatccttggc aagcttgccg
     8461 gatcgctgaa cactattctg gattacgccg gttcggatct ggataccgtg accagcttcc
     8521 gtcagcatgt ccacgcttac aagctgctgt ccgaccgggc ttcccaggat caggatgtcg
     8581 ctcttttacg cagacagctg accgaagaat tttatctgct ctactccctc ctgttcaccc
     8641 agacgctgga acagccgaac attcccatgc cggtcaaaat gtttctgtat ttcggttatg
     8701 tggatgagga gctggccggt ctgaaaaatg cagtcaccct gtaccgcatg gcagaagcca
     8761 tgtcggatca cagcgatatc ggtgtctata ccttttatga ctggctgatg gccatcttcc
     8821 gcgggaagaa atctcccagc cggaacgaat ttgaccagga ttattccgat tatattcaca
     8881 aacaaaaggt cggtggcaat attacggatg cagagctcaa ggcattggag aacaatccca
     8941 tggccaaggt caattacgag ctccggaata tgttccctca ggtcaacaag atcaccttcg
     9001 gacggatcac tactttctgt ccgctgttct gcgcagatga tgtgctgaag gatctggaaa
     9061 gctcctatgt caccgtttcc cgtgtctccc agattctcga ggagatccgc cgggtagatt
     9121 acacggcatt ctatagagaa agtctggata tggagcacat agatgtcatg ggtaaggaga
     9181 caattcatct ggaatatctg ccggacatca tcctgatgcc caacgtcggt atccgcggtg
     9241 tcatgtggca ggaaattgag ggcaagcgcc gtaacagccc cggtcgtatg gtcttctcca
     9301 ttttccatct ggaggatctg aagacgactt tcacccacct gaccggtgaa ttccgttggg
     9361 agctgtgcaa gagagtccag ggtaaccgct ggaacgatgt ctccatcagt tccctgacca
     9421 gtgaatattt cgattatatc cagttctacc gcaagaacca tgatctgagt acagaggcca
     9481 aggaaaaggt aaaatccagc ctgcagcgtg ccaagaacag tttcaaggaa atgttcgtaa
     9541 gggattatat gatctgggtg ctcttcgaag gtgccggttc cccgagactg aacaaagtag
     9601 cccgtcagat catgttcacc tactgcccgt tcccggagga tatttgcaat acgctggctc
     9661 agaacccgtt gtatgccgat cttctggatc gccgtaagat caagatagcc caaggcttac
     9721 atcatctgga tgttctgacc cgaaagctgc aaaacggtaa tattccggtt cccgagaccg
     9781 tggcacagga acgctattat ttaagtggca gcaaaaaagc atagcacata catatagcaa
     9841 aaagattcac tctccacccg gggaatgaat ctttttgtta tgttgtctta tttgctttca
     9901 gataccttat gcgtttgcca gctgtatacc aagttctctg cactctgaca ggccatcctc
     9961 gtccggtgtc tcatggcaga tcaggccctc accgttcaac acagtagctc ctgctgcttc
    10021 catacgggtg gtccagtctc tcatccactc accgtctccc cagccatagg agccgaacag
    10081 tgcgatcttt ttgccggaag caaagccttc cacatcacat acaaaaggct ccatctcgga
    10141 ttcctccagg acttccgctc ccatcgcggg acagcccagt gcaaacttgg gagcagcctt
    10201 tagttcgtca aggttggcgc cggtcacaaa tactacctcc gcttccttcc cggcttcccg
    10261 gataccctct ccgatggcct ctgccatagc ctgggtgtta ccaccctgtg tccagaatac
    10321 tacataaatc ttatccattt atttttcctt cccttccgca cctttccgtg cctgtttcta
    10381 tgctgcggta catccgcaga tatctatgat ttctgtcatc tttaatccat aatgcaacag
    10441 ctggatcaaa ttttccctgg catattcata tttttcaaaa agtctctgtt ttccttcttt
    10501 atccgcatat actttccaat aaccggtgca gaggctgttc ttcccctgat gtctgataaa
    10561 tccttccaca tcctccgggg gatatcctaa cagcaatccc agttcgtggg ggaacggctt
    10621 ccctctctct gcataggttt cataacgatc gcaaaatacc ggcaaaatat cctccagtcg
    10681 atcggattcg taccctgcct cccgcagcag tttctgtacc tgcggcattt tcagatactt
    10741 ttccagccgg tcttcccgat acaacagtat taccattttc tcctctgtct ccaacaaggg
    10801 atacagggaa agcacactgc tttcaacgat ccttcccacc tgtgtaaagc cttccgggga
    10861 aatcgtcagc agattggaaa tcttaagtcc cgtgatcaac ggtgcacact gcaaagccaa
    10921 ctgtagttcc acatcgtctg cttccatttc acacaggatc tcccacgttt cctgactcaa
    10981 tcagcgcttc acctcctgcc atccaaaagt cattgagtta gtcgtagcta acctatataa
    11041 ataatataga aaaaagcagt tcccgtcaag ggaactgcct gatagaaatt ctcaacatat
    11101 aaactttcaa aatagctttg gagatttcca ttctcagatc tttctgcggt ccagccctgt
    11161 actgcgatag tattctgcct tgtcctgata catccgtctc tcggactccg tcagtagatg
    11221 atcctttccc acggcttcca gagtgttcac cgctgctcct actgccatgg agacatcatg
    11281 agctgccagc tctgttttta attcctccac cttttgatag aattcctttt cagaaattcc
    11341 ggtgcacagg accaccagtt catctccgcc gatacggaac attccatttc cttcaaatac
    11401 acgtttcagg cagtcacagc acctgcggat caggcggtct ccggcggaat gtccttcact
    11461 gtcattgatc cgttttaatc ctgtaatatc acagtatacc acgccgatgg gctcggtatg
    11521 gtccacctgc tcaatgtacc tctccatagc ataacggttc cccaatgtcg tcagcggatc
    11581 cgaatagctc atcacttcca gctcccgtac cagatttctc tgcttcaggg acgatacgat
    11641 aaagtgtccc atgatctgca gcatctccga cacaaattca aaatatctgg tcgaaggatt
    11701 gtctactccg tagaagccga ttactttccc atcatcataa agcggaacca ccaccaggga
    11761 atgaatattc tgtcttacca gaacttcata catctgcgga tccacatctt tcatttcttc
    11821 cagattctct gtgacaatac tctgattctc atcaaacttc cgataccatt ccgcacagat
    11881 ctccggcggt agatcctgaa gggtatcctt ttccggtgcg actcccttgg ctacccattc
    11941 gtatgtattg tggtcatgat cgtggtcatc cttttcaaaa atataggccc gtcccgcttc
    12001 cagattcttt ccgacaaatt ccagcagcac ctgcaatgtc tgatccatgc tctcctgccg
    12061 ctgtgccaca cggatggctt cattcaacgc aacctccata ttacggtaat cttcggattt
    12121 gtgccctctc ccattcagat tgccacaatc tatggcgatt tccatacggt attttctccc
    12181 gtcttcttct accagtgtgt ccttgatatg aagttctctt cgcagcagcg gattgtaata
    12241 ttcccattcc ttaaaaaaac ccggctttaa ctgttcatta ttacacatgc cacagggcag
    12301 agaatttccc tgcaatatct cataacactt taacccgatg atctcttcaa gactttgaaa
    12361 tccatagctc ttcagagtct tcttgttcat atatatcaat tcatgagtct ctgcatctgc
    12421 cacatataca tattcaccca gattctcgaa aaattcccat attttactca tatacaccaa
    12481 cctgcttccg tattcttccc ctctttataa ctaatcagta tacacttgca aacgctttac
    12541 ggcaagcctg ttctctcaaa aatgtctgta ataatcttac atcatccgtg atcacaccgt
    12601 ccgccccaca gtttacgatc cgaagcatgg caccggaagt attggctgtc cacccgtata
    12661 ccgactttcc gcagtaatga atggcatcta ccatatccac tgtaagattt ctcccctcga
    12721 tactatagct gtcggcatag tccagctcat aatcctcttc ttccagatca tgggcaataa
    12781 aaactgttga aatccccggt tccagctcct tcattttctg caggattccc ttgttcatgg
    12841 agcctataat acactcatcc accatatcat aatcctgcag cagagtcagc acgctttcct
    12901 ccagtgcatt ctcctgtccg gtatatttca gttcgatcat cagagtgatc ctgcctctgg
    12961 cacaggcaag catttcttcc agtgtcggga tctgttctcc ccggtatgct gccgaaaaac
    13021 cgctgcccac ttccagggtt ttgagagcat ctgcctccgc atcccatacg cagatatcct
    13081 ctcctgtggt ccgtttaaaa ttactgtcat gcataacgat cagggttccg tccgacaact
    13141 gctgcacatc aatctctgca ataggcgctc catcccggat ggcctgttcc agggccgcca
    13201 ccgtgttctc cggagctact tttgcccccg cccgatgcgc taccacaatc acaggagcgt
    13261 ctttacttgc ggcctgtacc tgcggcaata caccatatgt cagaaaatag aggcagagaa
    13321 gcataacaat cgctgttttt ctgataattt tcatcacatt gccctcccat gaaagttatt
    13381 tgtcaatttt attgtaacta ttgactataa actccataca aggaaaatgt taactttttg
    13441 taaatattca gagccacgta aatataaccg gaggaacgtc gaatgcttgc attcgtaaga
    13501 tcctctggct atatttatgt ggcgaatggg gttctgaata ttacgactat tcagagccat
    13561 gtagcttcag gcagggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcga
    13681 atggggttct gaatattacg actattcaga gccatgtagc ttcaggcagg gtattcggca
    13741 gatgcttgca tacgcaggaa tatctgcctg aagttatagg gcgaattatc gttctgaatc
    13801 gtcggtaagc ccggataagc gtactcactt ccgtaacatc actaccagct catgagccag
    13861 cagtggaaat gctgccagta cattcaatag cagccattcc tgtccggaca gatgggaggt
    13921 tccgaacgct ccgatgagaa acggtacttc cgttaccgca aactgcagcg caaatccaat
    13981 aacacaggcc aacaccatca gcggattatc ccagggtctc atggcaaata cggacttctg
    14041 catatcccgc attcccactg catgatacag ctggcacatt cccagtactg taaaagcata
    14101 agtctgggat cgggcaagta ttttttcccg ggacagtaat tcacgaagtc cctctaccca
    14161 gtcagacaga ttccaggaca gttctcccct gcctgccagg atcccacagg gcaataccag
    14221 aaatgccgcc agaccgatac cggtgatcag cgctccatag agtaaggtac aggccattcc
    14281 gccccgggcg aacagactct ccgctgcttt tcttggagga cagcgcatca ggctttttcc
    14341 gtcattttta tccactccca gtgccaacgc cggaagggaa tccgtgatca gattgatcca
    14401 cagtatatga ctggatttta gaggggaagc cagccccaga gccactgcta cgaacatggt
    14461 catcagttcc ccgagattgg aggacagtaa aaatatcacc gacttgcgga tgttttcata
    14521 gacgcctctg ccttcctcga ttgccttttc aatcgttgca aaattatcat ccgtcaatat
    14581 catgtccgcc gcctgtttag ccacatccac gccggttccc atggctacac cgatatctgc
    14641 tgctttcaga gaaggagcat cgttcacacc atcccctgtc atggccacaa tctccccatt
    14701 ctgctgcagc cccttcacga tccgtacctt ctgggccgga gtcactctgg cataaacctt
    14761 gatattcctg atcctcctgg caaaagaagc atcatccaga tgattcagtt cttcccctgt
    14821 aatacactga ctgcgatgcc ctgcaatccc cagctgtctg gcaatggcat aggcggtatc
    14881 cacatgatcc ccggtgatca ttactgtaga tacacctgca cggcggaaaa tctccaccgc
    14941 ttcctccgcc tccggtctgg caggatcacg catccccacg attcccagga atgtcatacc
    15001 ttcttcccca cacccgggag tgtttccgct ctgtgctgcc gccagtgtcc gaagcgccag
    15061 ggaattcatg agttctacaa cctcactgat cctttttctc tgtccggctg tcattggcac
    15121 acttcttccg tcctgtaaaa tatgggtgca gtgctgtacg atcacatccg gtgccccctt
    15181 cgtataggca atgcttccct tatgccctcc gtttttcggg attctgtgat aggtcgtcat
    15241 catcttccgg tccgaatcga aaggcttttc atcatagcgg gggtactgct tctccagacg
    15301 ttctctatgc atccccatct tctctccgaa gtctaagagc gccagttccg ttggatctcc
    15361 gatccgttgc tctccctgca gggaagcatc gttgcacaac agcattcccc ggagcatctc
    15421 ctgtacgcat cgggattctc ccctgccatt gctgccggca gcatcctcca gcacatcata
    15481 ccaccagcat ttttccaccg tgagccggtt ctgggtcagt gtgccggtct tatccgagca
    15541 gaccacgctg accgctccca gagtctcaac ggaaggcagt ctgcggatga ttgtatgtac
    15601 tttgaccatt ctggtcactg acaatgccag acagatcgtc accactgccg gcagcccttc
    15661 cggcaccgca gccaccgcca gggaaattgc aaccagcagc atttccggaa tatttctgtg
    15721 ttgaaatacc gccagcagaa acaatgccgc acatactccc agagataaaa tactaagaag
    15781 ctttcccata tcccccagac gtttctgtaa cggtgtcatt tcctcttccg catctgtgat
    15841 catggaggcg atcctgccga tctccgtgtc catgcccgta gctgtcacga ttccttctcc
    15901 gtggccatgg gtcaccacag tagacatata taccatattg acccgttcag ccaatggaat
    15961 ctcgtgtctg cagtctgcca gaaacagcgc atctttttcc acaggtaccg attctccggt
    16021 cagggcggat tcctctacct gcagattgga gcttctggtc agccgtatat ccgcaggtac
    16081 ctgtgcccct gcctccaggt ctaccagatc gcccgtcacc agttccgccg ccggaatctc
    16141 ctgacggata ccttccctga tcacaatggt ctgtggactg gtcagctttt tcaacgcttc
    16201 cagtgccttt cgggcttttc cctcctgcag catgcccacc agggcattga ccactaccac
    16261 agtgccgatg atcacggcat cactgagctc tcccagaaag acggatacca cagctgcgat
    16321 caataatatg tagatcagcg ggtcctttaa ctgctccaga aaaagctgta ttttcgtctt
    16381 ttttctggca gctttcattt catttctgcc attttgctgt aaacgacgaa aggcctcacc
    16441 atcggtaagg cccttcactg cgtcactttt cagttcattt acggtttcct gtatattctg
    16501 tttttcgaac acggctgcct ccaatgcttg tttctattaa gcattgtatg tcatatcttc
    16561 agaattcatt acaccgaatc atcgtacatt ttaatctctt aactggaatg ctgcggtcag
    16621 ctcgttcatg ttggtggcct gcgcagacaa ttcctcactg gtggcagaag tttcttctgc
    16681 ggtcgcagag ttgttctgca ccactgtgga tatctgttca atgccctgtt cgatctgcat
    16741 catggcatct gcctgtgcct tggattcttc catggctttc ttggactcgt tggccagatc
    16801 ctcaatgcct tccacgacct tctgcaatgc ttcggctgtc ttgtccgtga tctggttgcc
    16861 atgctcaatc tcctgcaggg aagtctcaat caattctctg gtatgtaccg cagactgtgc
    16921 actgtcatct gccagcttac ggatctgatc cgcaaccact gcaaagcctc ttccggcctc
    16981 tcctgctctg gctgcctcaa tggctgcatt cagggacaac agattcgtct gggatgcgat
    17041 atcctcaatc tcaccgataa tatcgctgat ctgcttggaa gtatcgttga tacgctgcat
    17101 ggcatctgtc agacccttca tttcctctcc gctgtccttc gcctgctgct ggtaatccat
    17161 agccttctgg taagaacctc ccagtgcctt ggcattcttc tccacgatct cggttacatt
    17221 cgtgatggta gcctgcagtt cctctacgga accggcctgg tcagtagcac cttccgcaag
    17281 tccctgggca gcatctgcca tctggtttgc ccccagagat acctgtccca ctgccgtctt
    17341 gatctcatta agtgtctcac tgagcttatg gttcagctta cggatagatt ccaacagtcc
    17401 ctgaaagtct cccacatagg cttctgcgca acctgtagtc agtgcaaaat tgccctgtga
    17461 catctctccc aacagataat ccgcatcgcc aatcactttc tgcaggttac cggataatgc
    17521 ggcggtagcc tctgccagga tctgtgtctc atcctgtgtc cggatcactg gtgccggagt
    17581 atgcagatcg ccctcagcca acagacgtaa tctctcggta cactttgcca ccggttcacc
    17641 gatcgcctga ccgatctttt ttgccctgcg cacactgaaa cataaagctg cagcacctat
    17701 tgctacacca atgacaatac atacaacgga agaaccaagg aaatctgtca gtggtgcatt
    17761 tactgccacg ctccatccat tggttccttc tattggtgca taagcctgta ttgtgatggt
    17821 tccgcctttc ctataagtgc catatccgat tgctccggtc atcatcttct gctctacctt
    17881 tgcaattccc cgcaaagatc tgtccgtctg agacagttca atgttgctct tctgctcctg
    17941 ggcaatcgca gaatcacagt gagcaatggt gatacctgtt tctcccaaca gatagcactc
    18001 ggaattctta ctgacagaga ctgccgcagc aatatcattt aagaactccg gatccggcac
    18061 aacgaatacc acgccgacaa tatcaccatt cttatctccg tctgcatata caggtgcgga
    18121 aatgataacg ctgagcttac cgtctgtctt tgcaatcacg ggatccgata ccacgacctc
    18181 tccctgcatg gatctcttaa aataatcacg gtcactataa tcggttccgt cataagcaca
    18241 gataccgttg gcgccgatca gcttgcctct gaccattcca taagcttcca cacgctggtt
    18301 gatatactgc tgcttctgct cctgggtaaa tattggagaa gacagctgat agctgcatcc
    18361 gagttcggat acgatcgttg ttgtcacccg tagttcctct ttgatacggt ctgcagagat
    18421 ctctgccacc accagcatat cgtcttccag tgttctcatg gtactggaca gattcaggat
    18481 cacggtaagg atgaccaacg gaataatcga gaacactacc agcctgctga ttgccttctg
    18541 cagacgttcc cggatcgtcg tcatgccggg tgtctgcccc tttgtgccct tctgtgatgc
    18601 ttttgtactc atatgtcctc atttctgcgc tgcattttta ttatatgctt tattttcctg
    18661 tactctgtga agtgaccact tgtccggcag cgcccggata tcccatgcat ttccttcatt
    18721 ttggaacagt gatcttctgc agagatactt aattttatat gttgatttta ttttaccatg
    18781 attcagaaaa attttctatc ttttgaagcc caaaaagaga gttaatattc cgttaagaat
    18841 atcaactctc tcctgtcatt tatctctcta tcccctcatg ataagaaata ccctgttaat
    18901 agccgaactc ctgggcaaaa tacaacttgc cattggtctc atagaccgca atgctgcagg
    18961 tggtaaaatc gtctgccagg atattcgctg cgtgtgtagg agaagccatc caggcatcta
    19021 ctacactctg tgcggaatca tagcccttcg ccagattctc gccccacatc agttcactgt
    19081 tcactgtgta ccagtcctta ccgttgggtc tggtatggga gaacttggaa gctgcttcgg
    19141 ttgctcttac catagcgcag gactccagac cgttgctcca gttcagtgct gccaggccgt
    19201 tctctgctct cttcgcgttt acaatatcca acgcatcgat agccatggca cgaagttcat
    19261 ctgcacgggc cagctcagct tcactcatac cggcttttct cacagaaccc gccagtgcca
    19321 ctgcctcttc atcgaaccag acagttccgt catccgtggc aggatcacgg cttacagagg
    19381 ataattctgt actcttgccg cagcctgcaa ggctcagcac catgatgact gtcagaacaa
    19441 gactcatcca tctttttttc atttgttcta cctcttcttt ccttgattgt ttatagtaaa
    19501 tatatgttta ttcgttatga ttgcttcttc ttaataataa gccatgctcc cagccctgtc
    19561 aaggaaaggg caaagcccag attccatgta ttggaatcat cgccggtcct gggtcttctg
    19621 gaagctccca ggactgctct gtccgccaat ggtgtaggat catcggtaat gcttacaacc
    19681 cgctgacgtc ttacaccaag cacctgaggt gttaccgggg ttgccggctc agacggtgta
    19741 ccgggagtat ccggggtatc ggaatcatcc gaatcgtccg gagtatccgg gggtgcggtc
    19801 ggagtagtgg tcggcgtcgt gggtgtggta tccggtgtgc ctgtcggcgt aggctccgga
    19861 gtagcactgg gagtcggttc cgggctggta gtcacgggcg gagtaacctc cggctctctg
    19921 gacaattcaa actgcattcc gaagccgtat ctggtcatct gatagccgtt gttggccaac
    19981 acaccgttaa ggttagtggt gatcttcact tctccgtcct ttaacaggtc ggatacccga
    20041 gtatctgcat aggaaccttc ctgtccgttt ttcagcatcc ggtcatacag accttcaccg
    20101 tcaaaagtat atacattatg atatgtcgtg taatataaag catctgctac tgcagaacag
    20161 ctctccgcta ctgtggtatc ggcggttccg ttggtcagga ttgccagttc ctctgccgta
    20221 agttctgaaa agccgtagac cttatttcct tcttctctgg aagtattgtt gaaccagtct
    20281 aagtaatact gtgccaggca gctctgcgta acctcttccg gcttcatttc ttcgctgcca
    20341 tatccttttt tcaaaagagc ctctccgaca atcgcatccg taaccttacc tgcggtgatt
    20401 ccgagatccc gtaacggagc atttaaaata cggcggggca atgtacagaa cgctcccact
    20461 tcaccgatct cataaccttc aaaaccactg gacagaaccg ttactttctc tcctgcctta
    20521 ggcgctgccg ggatcgtacc gatcgtggcc tttgcaggat catactgata ctccttatta
    20581 ctctcattta ccagctttat gttgtagtac agggtatctc cgggctctgc cgcatttcct
    20641 cctgccacgg taccgttcag ataaccgatg gcatcggaca gattgattct taccttgtcc
    20701 ggggcattgc tgtggatcgt aatggtgatc gttgcatcat aggtcaccct gccggtgccg
    20761 ctttcccaat gctgttccag ttccggagtt gtagtaatgg taataccttc ggaaatataa
    20821 tggaacactg cgccgttctc attctgttca aaagttaccg ttcccgtggg atattcataa
    20881 taatctactg cggatacccc gggaatcctg taatcgtatc cgtcataatt gatccaggtc
    20941 tctgtgggtg tggtcacata ccataccgtg gcaatggtga ctccactgaa ggatcgaccg
    21001 gtggtgagca actgattcat aaaactttcc gttacatttt caccatacaa gacgccgttg
    21061 ttgtcattca catactgttt tacatcctca agggtttttc cgcagttttc tttataatag
    21121 gaagtgcctt cgctgtctcc accgtaataa acatagtgta acaaatctgc gggcttgcag
    21181 tcagaatatt tgtttacatc taatgctacg aatccaacgc actgccagct tgccccgtta
    21241 cttccggtta actttccttc ggcatctaca cctgtattat ccacagaaag atatttaaag
    21301 gtttcagcat catccaaagt catcttatga cctgcaaaca atgaaaaatc tccgtttgcc
    21361 ggcttgtaat tattgggcca tacgttgggg ctggctacct gtcctcccac atattttgcc
    21421 atggtgcctc cagcaagaat caccgttttg atggtgggat aggttttctc ttttacagca
    21481 acaacgctgt cgtttccgct tacacttgcc atttcaacaa catcgttctg ctcattctgc
    21541 gttgcttctt ccgcctgtac cgtaaatccg gtgttggaga tgctgccgat catcagtgcc
    21601 gctaccagag tttttgccag tttggtctgt attacgtttt ttaacttcat ttctgtttcc
    21661 cctcaaaacc agtatttctg tttttattac ttctttttac tctcctaata agtaagactg
    21721 cgccaaatgg aaaaaaggac cagaatttga aaaatttttc aagattctga tccttttata
    21781 cttttacagc taagatcggc tgtcgttccg ttcctgttta atgttctttt cgcttaagaa
    21841 caagccatgc tcccagactc gtcagggaaa aggcaaagcc cagattccag gcatcggagt
    21901 catcgctggt ctgcggtcgt ctggatgctc cgaggactgc tctgtccgcc aagggaactg
    21961 tctcatcctc gatggtcacc attctgcggc gtcttgcacc aagtacctgc ggggt
//
LOCUS       DAWEJW010000043        21690 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_966_length_21690_cov_61.224728, whole genome shotgun sequence.
ACCESSION   DAWEJW010000043 DAWEJW010000000
VERSION     DAWEJW010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 21690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21690
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_966_length_21690_cov_61.224728"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12962..13061
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5155 a         5924 c         4694 g         5817 t
ORIGIN      
        1 ttttccgtaa agccggctgt ttttagtctg tcccacaata ccggcattgc ctccgcaccg
       61 ggaagcgccg gatttgtggg gatgccgtcg aagtcactgc ccagacccag tatttccata
      121 ccgccgacat cagtgatatg tcttgcctgc cgtatcagtg cttcccacaa cacctctgga
      181 tctttgcttc cgccatccac cagaaattta tcataatagt tcagtcccat acagccgccc
      241 cgctccgcca gtctgcggac ccggtcatcc gtcagattcc ggagggacgg acatacagcc
      301 cttgcgttgg aatggcttgc cacgaagggc ttcttcgtac attcccatac atcgtcaaag
      361 ccggcatccg acagatggga gacgtccggg atcattccga gacgctccat ctctgtgaca
      421 aaatcgcggc cggtttccgt cagaccacac gccgcatccc tgccatcgga tccacgtatc
      481 ccagtctcac ctttctccgt ccctatcgcc gttgtcggat tttcccccgc acagctgctc
      541 ctcctggttc ccggtatttt ctcctgaaca gatttctccg tcggtctccc atctacgtcg
      601 cccctgctct gcccaagaca ccgacgcgtg gctggccacc ccagttcatt ccgataattc
      661 caggttaacg tcagcatccg cacccccatg tcatacagtt cccgtagcct ctccagactt
      721 ccgccgcaga ccgccccttc ttccaccgtc aaaagtgccg acagtttccc acaggcttca
      781 tttttcaaaa tgtcttcata gcacagtacc gggacaatcc ggttcttgtt tttctccagc
      841 tccgtccgat agacctcata tagagcacac acccgctccc agggtgtcgg tgtgcacttt
      901 ccgatatcta caaacagggc gaaattctgc aacagatatc cgctctcctg catccgctta
      961 agatccagat ggccgctgtt tttccaaagt tccccacccc ggtccgccag ttcggatatg
     1021 gtgtcgcaat gcatatctat gatcttcatg ttttctcctg atcccctatg aaaaagagag
     1081 catccacagc tctatgttcc tgccatgaat actccctctt actttctttg ttcaatttat
     1141 gctgttgtca gcaaaaaata ccttagttct cttcaccctt gactgcgaag catacgccgg
     1201 tatcgtcggg cttatcctca tcaaacttgt agtccttgcc gggcaggata gcgttcagga
     1261 taatgccggc cagcgcgccg actgccagtc cggacaggct gatggtgatc tcaccgatgt
     1321 ggaatgcgat agcacctgct gcactatagt tgataccgat ggacagtacc agaatcagag
     1381 cggcgatgat aacgttacga ctcttggtga aatctacctg agtctcaacg atattacgaa
     1441 caccgactgc ggagatcata ccatacagta ccagggatac accaccgctg gtagcggtag
     1501 gcatacaacc gatcaatgct gctaccttcg gggagaagga gaacagtaca gcaaagcctg
     1561 ctgcaatacg gataactctg ggatcaaata ccttggtcag agacagtaca ccggtgttct
     1621 caccgtaagt ggtattcgca ggagcaccga acagggacgc gataatggta gccagaccat
     1681 cacctaacag agtgcggtgc agtccgggat ccttgatata gttcacaccg acggtggaag
     1741 aaatagcgga gatatctccg atatgctcta ccatggtagc cagtgcgata ggcatgatgg
     1801 tgatcacgga agtgatcagc agggaagtgt tgccgtcgga cagtgcccag aaggcggtct
     1861 cattccagtg aatgggaagt ccgatccatg cagcttccgc taccggtgtg aaatccacac
     1921 ggccaaggat ggctgccacc acataagaac cgataacacc gatgataata ggaacgatct
     1981 tcaccattcc cttaccccag atattgcaga tcactaccaa tgcgatagca accactgcga
     2041 tcagccagtc tgcggaacag ttgtcgatag cggactggga cagggtcaga ccgatggcaa
     2101 tgatgatggg gccggttacg atgggcggga agaaacgcat taccttgccg gtaccgaaga
     2161 tcttgattaa cagggataat accagataca gcagacctga gcaggccacg ccgaagcagg
     2221 cataaggaag cagtgcggaa ttgtccgctc cgtctgccat aggggcaatc gcggcataac
     2281 cgcctaagaa tgcaaaggaa gaacccagaa atgcgggtac ctttctcttg gtcagcagat
     2341 ggaacagtaa agttcccaga cctgcaaata acagtgttgt ggaaacatcc agacctgtca
     2401 gcatgggtac cagaatagtg gcaccaaaca ttgcgaacat gtgctgaagt cccagcagaa
     2461 gcatcttggg cacgcccaag gatctggcat cgtaaatgcc gttttcgccg agtgtagtct
     2521 tctcgctcat ttttttcctc ctgtgtcata gacatgtgaa catgtaacta tttttcgtcg
     2581 cataaattcg ggcagatagt cctctatctg cccacaccta tgtgactcta ttagttacat
     2641 gatagctttt caccgttcta cagtcaagag gaaaatcact ccaccgcatg ctctttcagg
     2701 aaatccagcc agataggaat atactgtgcc ttcagatgca caccgtcata agtataggaa
     2761 gccaccattc caccggtctc gtcacagatc agcggattta catccaggaa aaatatcttc
     2821 cggtcatccg ccagctttgc gatttctgca tttctggctt cgattccttc gttggtgata
     2881 tagtccccct gttcggagcg ttccgttgtt actttcatga tggcctgcag atagatcacc
     2941 gcatccggct gcaattcctg caaatgctgt accgcttccc cgtaagcctt cataaaagat
     3001 tccactgtgc cggtacccat ttcgttgata ccgatcatca gatagatctt ggcaaactgc
     3061 ttctccgaca aggcttcctc caccgtgatc tttttcttct gtccaggcac cgatacaatg
     3121 gcactgtcga acatcttgta gactgtcagt ccggtggaag cataaaacgt ggatgtctcc
     3181 tccagtccgc cgtactcata catgcctacc gtccgggaat ccccgataaa taccgcatca
     3241 tcaaaataac tgtcatccac cgtgtgatag accacttcct gcggtatctc ttccacagga
     3301 accgtcgcgg cgggatcctg cgtctctgtt ccggtggtct ccggcattgc ggcctcttct
     3361 cccgtagcgg gactctgcac cgtacccatc gtctgatccg tctggttccc gtcactcact
     3421 gtatctccgg tctgtacgtt ttcccccggc acattgccgg atacggatcc cgttgcatca
     3481 ctctgttccc cggatattcc cggccatttt ccttccacca gctgctccgc cagaagcact
     3541 cctttcgtat aggtctcctg cagcggttcc gtatatacct gccagttatc cgtccaggta
     3601 aagaatacaa ttcccgccag caaaagacat cccaaaaagg agtatttatg caatttttcc
     3661 ttcattttcg accaaatatt tttattttct gcatgattca ttgcataatg accttttcct
     3721 aaaagctaaa atacataaaa ggattatttc cttctaataa aagtctccag atgcaatacc
     3781 agaagagccc cgccagaagc accttcaccg gcagcttatc ctgccactta tggtacagct
     3841 tctcaatgac cggtgtgcag gccagtccgg agaccagaag cagtcctcca taggtactga
     3901 gtgccttcca ggcatcctcg taaccgccgc tgaagcccgg taccagccac agcatcttat
     3961 ccagatagac ctgtaactgt gacaggtccg tgatagcaaa gcacatccag ctcattggga
     4021 tgaccagcca cagataaatg tgcggcagta ccttcatttt atgtacaaat tttaatctgc
     4081 ccagctgccg ttccagtacg atgcagatcc agagcagtcc gccccagcag aagaagttcc
     4141 agccggcacc atgccagaaa gcggtcagca tccataccac caggaggtta aaaatcgtgc
     4201 gaagctctcc ctgtctgctg ccgcctaacg gaatatacac atatcggcag aaccactgtc
     4261 ccaacgtcat atgccatctg cggtaaaaat cccggacact tctggccata tagggtctgc
     4321 ggaagttggc cggcagttcg atccccatca tccggcccag tcccatggcc atcagggaat
     4381 agccccagaa atcaaaatag atctccatgg aataggcgat ggctgccagc catgctaacg
     4441 gtgtcgagat gctttcaaat ccggtcacct gcacctcgtg ccacaagatt cccacccggt
     4501 ctgccagtag caccttggcc gccagtccca gaataaacag cttcataccg tcttccagcc
     4561 ctcgcaccgt gaaagtgcgc tccgtcaggc tgtccgagat atcgccatat cttgtgatcg
     4621 gtccggagat cagtttcggg aacatgatca cataagtagc aaacttcaaa aaagaatact
     4681 ccctgctcac ctctccccgg tacacatcga tctgataaga caatatctgg aaggtgtaaa
     4741 agctgatccc cagcggcagc cccgcagagc ctccccagtc cttgaaccag agcagtaccg
     4801 ccagattcgc gccaagtgtc aggatcagca gaagcttttt ccgccacaac gtcttaggac
     4861 tgcgttttgc cagatgaaga cccagaaaat aattgcacaa tatagatatc attaaaaggg
     4921 gaagatacct gacatctccc atggcataaa aaacaatgct tcccacgagc agtgtcaggt
     4981 tcctgcattt gcgaggtgtg atcccgtaga cgatcagaaa gatcggcagg aaccacagca
     5041 gaaattcaat actgctgaat atcaaaaaaa tcacatcttt ctatttctgt ttatctgtct
     5101 tactatcata gtagcttgaa accgtttcta ttacaacaaa acaattctta attttaaatg
     5161 acagaatttt acaactgctt aatctttttg caatttttca ccgcatcgtt tacaatagtt
     5221 ttaacaccac agggctgcgc ccgagaaagg atattttcca tgaaagaaca gttatatacc
     5281 atccctttaa acgatgccgt aaatgcacag gacgaatgcc ctttctgctt tatccaccgc
     5341 agtattgaac aggatcttct ggatttcgtg ttaggctccg gttcctctta tatggaagcg
     5401 gacatccgcg aacaaaccga caaagcaggc ttctgccggt atcattttca gaaaatgttc
     5461 gactacggca atactctggg caatgcctgg atcttaaaga cccactacca gcgcatgatc
     5521 cgggaaatgc agcaggaatt tgcctccttc cgccccggca aaagctctct cctgggtaaa
     5581 ttcaaaaaag tcgaaggcaa tgaaaatacc atcggcatgt gggtccgtgc aaaagaggat
     5641 tcctgctata tctgttccca gtataaagat acctacgcca gatatctgga cacctttttc
     5701 tacctgtgga aaaatgatga aaacttccgc aataaagtca taaacggcaa aggcttctgc
     5761 ctgccccact tcggcgatct ctgcgaagcc gctgaccgca aactttccga taaggaaaaa
     5821 caggactttt acgatcagtt gctgccggtc atggaatcca acatgcagcg catttccgaa
     5881 gacgtctcct ggctggtgga aaaattcgac tatcgcaaca aagacgccga ctggaagagc
     5941 tccagggatg ccatccagcg cggtatgcag aaattaaaag gcggatatcc cgcagatccc
     6001 gcctataaga tgtctaaata gccgcaaaat tttattcaaa aatacgatgc aattatgatg
     6061 ctatttttcc gtaatttctt cgtcagaggg ctgtccggac cgttccgcca gtcttctgct
     6121 taattgcaga tactccagaa tttcctcata cagcatctcc ggctgaaagg catcttccag
     6181 gattctctcg ttcatcaatc ccttaatggt cagttccatc atcttacgaa gtttcaccac
     6241 gtcttccgca gccgcatatt ttcccagatc cgctctggta taaatattct cataagcctc
     6301 ttccatctgc tgttttcgct cttctaccgc caccagtgcc tcattgacat tctctgcctg
     6361 tgcccggttc aggaacggct gcagataagg atagccgcac atggcatgca ttttcccggt
     6421 ctccacctgc tgcatcagag tgaacagatc cgtctccttc gcatctaccg ctgtggacaa
     6481 ttccagcaac agataccgca cactgtagtc atagacaaaa gcatagactc ccagcttgtt
     6541 ctcaaaataa tgaaataata atcccttact gatggctgcc tcccgcacga tatcatccgt
     6601 actggcatga cggtaaccgt tcagggcaaa taccttcaga gctgcattaa tcatccggtc
     6661 ctgtttttct ttttttaaat caaaaaattt ctcattcata tcgcaacata tcccttttca
     6721 ttttcatcaa taaggtttat tatgtaactg tatgggcata tgcatgaaat tcagaccagt
     6781 tacatacact acaacatgga aggagcaacc ctatggctaa aaaatttggc aaatttctgc
     6841 tgtttaccgc agcagtaggt accgcagccg cagctgctta ctactatatg caacagaaag
     6901 attctcagct gaaggaatct tccgatgcag atgatgatta tgatgatttc cacgaggatc
     6961 tggacaagag taccgagttc tcccgcaatt atgttcctct gaatacaccc gtgcagacct
     7021 ctgccccgaa ggaagagaca ggcacagagt ccacaccggc ttccgaagag gcggcacccg
     7081 cttccgcaga ggcatctgat ttcacaccgc tcaccgatca ggctaccgga ttgtccggcg
     7141 aagagggcga gaccgtcgaa gacgtggaag agttcttcga tgaagaagat gccgaggacg
     7201 aagagcctcc cgtaaatgaa gaataatcgt tctatttcga aggtctcaga gccatgcttt
     7261 ttgcaagcgt ctgagacctt cctgcatttc cgcctcatca atacttccaa agcccaacag
     7321 cacggtagct ttccgcattg tggcttccac catattctct gacatcccat aggctttcac
     7381 tttctccgcc gctgctctct ccaacagttc ctgttccgtg acattccctt tcgctgtcaa
     7441 cagaagatgc agtcccgcat cctccccgga gatcacaaac tgcttcttaa acggttccag
     7501 cccggccagc agcaggtcat gtttggcccg gtagcgcatc cgcattttat tcagatgccg
     7561 ctcgaaatac ccgtcccgga taaattcatt caggatccgc tggtcgattc tggacaccgt
     7621 gcaggaataa aaagagcaat ccctgcggta gatatccagc agatcccccg gcaataccat
     7681 atagctgaca cggattgccg gtgcaatagc cttggaaaat gtaccgatgt agatcacttt
     7741 tcccttttca tcggaagatt gcagggaagg aataggcttg ccccgatacc ggaattcact
     7801 gtcgtaatcg tcctcgatca gataacgatc cggctccctt tctgcccagc gaagcagttc
     7861 tgctctcctg ccgatggtca tgaccgcacc ggtgggatac tggtgggaag gcatcacata
     7921 ggccactctg acaggcagtc ccgaaagtct gtccgcccgc attcccttat catccatgtc
     7981 taccgtgtga atgcgatagg caaaagattc aaagatgcgg tatgcccgct tgtacgtggg
     8041 attctccatg gcaatgccca catgtctgcc caatattttc tccaatagca acagcagata
     8101 atcgtttcct gctcctacaa tgatctgttc cgggcggcag ttcacccccc gggaagaatg
     8161 cagataccgg ctgatggtca gccgcagatc ataatctccc tgaggttctc cctgcgcaaa
     8221 caggtcactg ttgttgtcat tcagaatatt cttcgtgatc cgcttccata cgcccacagg
     8281 gaaaccgttc atatcaatac cataaggaga aaaatcaatg catccctctt ccggaacaga
     8341 ttcttctcct gaagtgtccg gctccggttc gttctcttcc cgcggttcca tggcaaatat
     8401 cccttccaga cggcagacaa aatagccctt ataaggtcgt gcctcgatat atccctcgct
     8461 taacagctgg tcgtaggcat agtccacagt gcttctcgcc acctgcagat attctgccag
     8521 agaacgcgtg gagggaagcc tctctcctgc cagaagcttc ccttccctga tttcctgtcg
     8581 tatatgctca tatatctgct ggtacagaca tttgtcactg tctgtacgca gccggatcgt
     8641 catatcatac atacggttat cctacttttt ctggttcttc agttcttcca gaatctggtc
     8701 ttttaactct cttgccttgt ccttagtccg ggcattggcg gaaggggacg tcaggatcgt
     8761 ggctaccata cggctggcca catcatattt ctggaatctg gcagcaagta ccgcaatcag
     8821 aaattctacc gtgatctcat ccataccgca catggggaat ccttcggtct gcagggcctc
     8881 cgtaaaacca gtgtaagcgt tcttcagata agtctcctcc atggtcttca cttcccggag
     8941 tctggccata tccgtatccc cggactcttc cagatgctcc tgataaccac gcatcagcca
     9001 tccgctcttc agacagatat aagccttttc actggcatgg gcccgtttta ccacagcgtt
     9061 ggccaggcag agcttatagc gctcaatggc ttcttcatag ctgtaaatat cgtcatcgta
     9121 ggtatgcagc tgcactttac tgctgatatt ttccttgatc agctttatat aaaccttatt
     9181 caggctgttg aaataccggt tcagtgctgc atagccgcaa tgcggacaca gaataacatc
     9241 atatttcacc gcatcaatgc cttcgtattt tgcacgaaga tcctgatcgg tacttaaaag
     9301 tcttgccttt ccagtcttca tgatcttcgc cgggaaatct tctccgcaga caggacaggt
     9361 aaatgtcttg tcaaagatca gatccttttc ttctattttt tgggcctcgg ctgctgcctt
     9421 ctcttctttt ttatcctgtc cgtaaatgtt catgttctcc aggtcatcca gacccagacc
     9481 ggccagtcca aacaatttgt ccgacatatg tctctccttt tcttttgtac tgtccattcc
     9541 cggtgacaat acgaatacaa ctttcccaat tccccttatc ggttgtccta ctgcttactt
     9601 aggcagtaca taagaactct tcagagatac gatacggtta aataccagtt ctcccggctt
     9661 ggtatccttg tagtccacgc agaagaagcc ctgtcttacg aactggaagc ggtcatacgc
     9721 ctgtgcctgt gcaaatgcag gctccagacg gcactctgtc agtgtggtca aagaattggg
     9781 gttcagattc agacttccgt cttcattgta tacacccttc tcttcatcga tgatattctc
     9841 atacaggcgc acctgtgctt ttacagactg ctctgcattt acccagtgga tcgttccctt
     9901 tactttacgt ccatcggggc tgttgcctcc gcggctctcc ggatcatagg tacaatgtac
     9961 ttccacgact tttccgttct cgtccttgac acagccggtg cattttacga aataagcact
    10021 catcaggcgc acttcgttgc cggggaacat acggaaatat ttcttgggag gctcttccat
    10081 gaagtcctct ctctcaatat acagttcttt gccgaaggga acctcgcggc tgcccagagc
    10141 ttcgttttcc ggattgttga ctacaggcag catctccgtc tgtccttcgg gatagttatc
    10201 aattaccagt ttcacaggat ccagaatagc cattatacgg ggtgcctttg ccttcagatc
    10261 ctcacggata caatactcca gcattgcata atctgcggaa gactgtgcct tggagatacc
    10321 gcacaattct acgaacttct tgatggattc cggtgtgaat cctcttctgc gcagtgctgc
    10381 gatggatacc agtctgggat catcccagcc gtccacgatg ccctgttcta ccagcttctt
    10441 gatataacgc ttaccggtca ccacattggt caggtacagc tttgcgaatt cgatctgctt
    10501 gggaggatga ggatattcca gttctcttac cacccagtca tataagggtc tgtgatcctc
    10561 aaattccagc gtacagatgg aatgggtcac gccttcaata gcatcctcga tgggatgcgc
    10621 gaaatcgtac atagggtaaa tgcaccattt gtcaccggta ttctggtgag acatatgtgc
    10681 cacacgatag atgacgggat ctctcatgtt gatgttggga gaggccatat cgatgcgggc
    10741 acgcagtacc ttagtgccgt cctcgtattt gccggccttc atatcctcga acagtgtcag
    10801 attctcttct acgcttctgg tgctgccggg atcttccttg ccgggctcgg tcaggcttcc
    10861 tctgtattca cggatctgct ccgcacttaa gtcagagaca tatgccttgc cttttttgat
    10921 cagctttacg gctgcttcat acatttggtc aaaatagttg gatgcgaaaa agagtctgtc
    10981 ctcccagtct gctcccagcc actttacatc agccttgata gactctacga actctgcctt
    11041 ctccttggtg gggttggtat cgtcaaaacg cagattgaat ttgccgttgt atttctggga
    11101 caggccgtag ttcagcagaa tactcttggc atgtccgata tgaagatatc cgttgggttc
    11161 cggagggaaa cgggtgtgta cagtatcgta tacgccctcc gccagatctt tctcaatgat
    11221 ctgctctata aaattcttcg attcctttac ttctgcggaa tctacgattt ccttttcgat
    11281 ctcgctcatg gtaatgtcct tgcctttcgt ttacttaata ctgttttcgt atcctttatc
    11341 ttaccacaca agaaaacttt tataaaccct tctcttcaaa ataattcagc aaattctgct
    11401 ccgtctcgat gaagatattg tgcagatcat cctcactggc cgccagataa tctccggccc
    11461 ttccggtcat atagccgtcc tcgtcccata ccgggaacac ttttaccccc tgtgtcaccc
    11521 cgatggcata gatgcggaac tcatccttcg gcatgcacat gcgcacatat tccgtgatca
    11581 gatgatttct gccgtccgta ccgtccttga ccctgggaac gtatgtcata ttcctgcagt
    11641 agtcctcgga caccggagcg tcggacagtt ccaggatcct gtggaaccgc tctcccgcca
    11701 ccctgtgaag ttcaccgttt ctctccagcg tgtagcagat attctgtctg gtatcgatgt
    11761 ccatggtgcc gttctgggtg cggacggaca ccaccctgcc aagctccgcc agatcagagg
    11821 ccctcacaca ggccagaggg attttctttc tctgatatct tgtgccctca cttaccggga
    11881 atgcccggtc tttggcgtag aggatccgga acgtatcgaa atactgcgtc atgcggttat
    11941 tgaaatacgc ttcggaatgc agtgttggat atttttcctc atataatttc atactgatgt
    12001 agccgccggc cttctcgtaa tgtccgccgc cggaaccgat gccctccgaa agataatccg
    12061 acagttcgct ggcattgacc tcccggatac agcttctgac ggaaaatttg tatccgccgc
    12121 tgtcctcatt atagaccacg caggtattga cgcccgccac ctgcaggagg aagtcgctga
    12181 tcaggccaag tacattggga tcacagggct gggaatggat cacggcgaac tggtaatcgt
    12241 cgttataatt gcagcgcagc attgccacac cggcaatttc cagctctctc agggaaatat
    12301 tggaattacg gaacagggat atctggttct tatcaaaatt cagactctca cgcatatcca
    12361 tgtccaccgg gttcgacagt tcggagaact ggttggtatc catatacagg ccgtaataca
    12421 gcgcagttcc cagatctctg tttttttcca cgggataatg catttcctgc agcattttcc
    12481 acaccagggt tgcacagctt cccatattgg gctgcagacg catccgctcg gtgcagatga
    12541 cttccagtgg atgatggtcg atgacagcga tctcctccgc cgggatcttc gtcacattgc
    12601 cggcaccgta ctgacagtcc accgtgatca gcagtccatc tactgtatct tccatctgag
    12661 gctggtactc cagcggaatg tgcagtttct ccaccatcag catcaggttg gcctttctga
    12721 ctttattccg tccggaatac agcagcctgg tatcctttcc cttgtcccgg aaaaaacagt
    12781 acagcccata gccggaggct atggcatccg catccggatt atcgtggcac tggatcgtca
    12841 catggtcata acccaataat tcatccagca tcatttctct gctctttgca tcccctcgtc
    12901 tgtccataac tacgctttcc tcttcttcct caaaaaacta ttcagaacca cattcgcaaa
    12961 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natctgtctt agtggtggaa
    13081 cagacggatg cccgtaaatg ccatcaccat atcatatttg tcgcagcatg caataacatc
    13141 gctgtcacgc acggatccac ctggctgtgc gatgtatttt acgccgctct tatgtgctct
    13201 ctcgatgtta tcggagaaag ggaagaatgc atcgcttccc agagtcacat cctgcatctg
    13261 atccagccat gctctctttt cttctctggt aaatacaggg ggctttacct tgaagatacc
    13321 ttcccaggta ccgtccgcca gcacgtccat gtactcatcg cccatgtaga catcaatggc
    13381 attgtcacgg ttggctctgc ccaggctgtc tacaaactgc aggcccagta cctgaggaga
    13441 ctggcgtaag aaccagttgt ctgccttgct gcctgccagt ctggtgcagt ggatacgaga
    13501 ctgctgtcct gcaccgatac cgatagcctg accatccttg acataacata cggaattgga
    13561 ctgggtatat tttaaggtga tcagagcgat cttcatatcg atcagggcac tctccgggat
    13621 ctccttgttc acagtcacga tattggacag cagatcgccg ttgatatcca gttcgttacg
    13681 cccctgctcg aaggtgatgc cgtatacctg cttgcgctcg ataggagcag gctgatagga
    13741 aggatcgatc tggatgatgt tgtaggcgcc ttttttcttc tgcttcagca gttccagtgc
    13801 ttcgtcggtg tagccgggag cgatgacacc gtcggatacc tctctcttga tcaggcgggc
    13861 ggtatcggta tcgcagatat cagacaatgc aatgaagtca ccgaaggagg acattctgtc
    13921 tgcaccacgt gctctcgcat aagcacatgc caggggagaa agctctccca gatcatccac
    13981 ccagtagatc ttcgccagag tatcactcag aggaagtcct accgcagcac cggccggaga
    14041 cacatgtttg aaagaagtgg ctgcgggaag tcctgtagct tccttcagct cttttaccag
    14101 ctgccagccg ttgaaagcat ccaggaaatt gatatatccg ggcttgccgt tcagcacggt
    14161 caccggcaga tcactgccgt cttccatgaa gattctggaa ggcttctggt tcgggttaca
    14221 tccgtatttt aactgaatct cgttcatctt atttttctcc tattctattc tacgatatta
    14281 actattcaga acctgcattc gcaaaacgcg tcttcacaac ctactttaca tattcttatt
    14341 cacgatcttt gtctcatatt taccggtctc gatatcaatg aaacgtacaa acagagagac
    14401 cttgttatcc tcattcaggc tgttccacag catatcggta aaagcatcca tatcatcggg
    14461 gatctctacc agtttcggct cgccctggaa gctgggcagc ggattgccgt cgtgcatata
    14521 agtgtgaatg aaacgtccct ctccgggaag aggattctca taagcataag tgaaacgcag
    14581 acaggaatca ggattgccgt tgtcactctt cagaatggac atagcgtaat tgtacttgcc
    14641 gttctcgatg tgcatgatac cggaaattct cggtgtatag ttggggccgt ccggctcgaa
    14701 ttctctgctg cgcagcgact gctcaaaggt cagctgtttg tccattccct cgtagatagt
    14761 atctgtctgg tcaccgttgg tcacgatggt cttgttacca aggacacgta cgggtgcgta
    14821 gatgatcaga ctgggatctt ccagtttgga aggatcgaaa gcctgggtac ggattccctc
    14881 tccttctgct acgaacacac gattgcggct gttggcgctt cttcccatga tgaaataagc
    14941 ggtcacggca tatttaccgt tagcagaacg tccgatcacg atacctctgc cgggataagc
    15001 attgcttttc aattcctgtt ccagttgtaa catagttttt cctccgttta ctatatatcg
    15061 cgtaactatt cgcttcacca gctctgaata gttacaaaaa accgcctaag caccagaaat
    15121 agacggtgcc caggcggtcg gctttcacta ttctacactc cctgtgggta tctccactgc
    15181 agcaagctgc gctccgccag tcgtgtagct gttttaatag tagcatggtt ccccgatact
    15241 tgacaagagg gattttgata attttgttgc ttccgtaaca gttctgcccc aaaacattca
    15301 taaaagcgtc tgctgcgcag ccgtcgctgc tttgcagctc atcctttatt catgttttgg
    15361 gcgctccgct catagaatag caaaagcgat gctccgtgtg caagcacccg atcatcgctt
    15421 ttactattct atggctgaac tgttacttca catatttttc ctcaaatgca tgcctctcca
    15481 aaggacggtc aaaataatag ccctgaatgt atttgacctt cataccctgc aatactctga
    15541 actgctccgg ggtctcgata ccttccacgc agacgctgac gccgatggtc tccgccagct
    15601 ccgccaccat tttgataaag gactgggagt aggcatcctc cgccagatct ttgacaaagc
    15661 tctgatccac cttaatgaca tcaatgggaa tctcccggat atgattcagg gaggagtagc
    15721 cggtaccgaa atcatccagc gccagcgaaa cacccagaga tttgatccgg ttcaggatct
    15781 ccttcatgcg ctccatatcg ttgatagcga ggctctctgt cacttccagg gtcagattct
    15841 tcggggacag acctgtgtcc gcaagcgcct tttccaccgt ctccacgatg tccggctgta
    15901 ggagctgtac tacggaaaga tttacgttca ctttataatt cggataaccg gattcgttcc
    15961 agcgtttgca gcgggtgcag gcctcggtca acacataatt gccgatggga ttgattagtc
    16021 ccagatactc tgccaacggg atgaattccg ccggggagat aaatcccagc tttgcactgt
    16081 tccagcggat cagtgcctca gctccggcgc agacatccct gccgccctga atatcaatga
    16141 tgggctgata atagacctcg aactcccggt agccctccac cgtggcatca cgcatgttct
    16201 tttccatatc caggcggcgt cccgatacgg aatcaatgcc ctcgcggtag gttgccaccc
    16261 ggttcttacc ggtctttttt gcttcgtaca tggcaatatc cgcttttttg atcagatcag
    16321 ccacggaatc cccggcatcc gggaaagtga cgatacccat actcatggta cagtaatagt
    16381 ccgcgtcctt caaaaaccag ggcttgctga agatcttttt aatatcttcc acaatatttt
    16441 caaaccggga atagctgtcg ggcggaatca cgatgacaaa ttcatcgccg cccatgcggt
    16501 aacaggtatt ctcgatgccg ttgatccgtt ttaatgcatg ggaaatggat tttaacagca
    16561 catctccgta ctggtgcccc agtccgtcgt tgatatgctt gaaatcatcc agatccagat
    16621 acagcagagc accttcgccg ccggtcttct tcgcctgatc gatctgtctt gccagatccc
    16681 tctcacagca catacgatta tacagtcccg tcaggaaatc cgtataggcc tgctgctcga
    16741 tgcggcgctg gtacagtttt ttatctgtaa tgtcatacag agagtacaga ttggcctttt
    16801 taccgtccac ccaggcaatc tccttgcaca gcagatcgta ccaggtctcc ttctccgcat
    16861 ggtagactac actgactgac ttggtttttt ccttgcggac actgctctgt aacaaagcat
    16921 caaaattgtg atctagcagt tccttagcaa aggtattttt caggatctga ttggcaaaca
    16981 gcatccgtcc tgtggtctgg tctgtcacat aaatggcaca gcccacgttg tccagaatct
    17041 cctccaatgc tgcgtaggaa ccggccaggg aattcttctg gatccttctg gtgaggatgc
    17101 tctgcaggat cttcactgca tccgctgtga acttgatctc cgccatgctc cacacatgcc
    17161 cctgggttct atgattcaga gacagcacca tatttccgct ctcctgcttc aggatcggga
    17221 agatcatcac ggccttcatg ccgatctcct cgatttcctt cgagccggca ttgcccaggc
    17281 tgtccgtgga taccaccagt ggtttctcag catgaaggaa ggaatatgct tccactccgt
    17341 ttatcttgtc aaaataagaa atctgtcccg gggcacgcca ttcacagacc acgttcatgg
    17401 tatctgtgtc ggaatgcagt tgaaaaatat ccgcagtatc tacacggata tgctctgaca
    17461 ggatcttcag ccacttattc atggcaagtt caatctgttc atcactgtct aagagctgga
    17521 cgatctccgt ggtcgcctca atagtatgga gattccgact catctcttcc tcagcggaac
    17581 ggctccttct gctctctgcc tccgcgctga agcaggacat acgatctctg taaatatcta
    17641 cgctggtatc ccgcagcaga tccaatatct gataaaagga aatggtgtcg atcttcttca
    17701 gatcatacac ctgccagtac aggatcgtgt tgttctccac actgacagcc actgctgcca
    17761 catgtccgcc gtccggcagt tcttccacca cctgttcctc cagcgaaccg tcctgcactc
    17821 tgtccagtgc ctgtgctgct ttcctgcgat attccgtact gatcgggtct tccccatcca
    17881 gtctcttcca tgtcttgtcc acatacggct ccgtcaccac ctgattctca ctgtccaggc
    17941 agacagctgc cacgcctgtc accttgcaga attcattctg caaatgctgc agacgtctgg
    18001 gatcgatcaa ttctcgaaat gaaatttcag ccactttttt gctcccctgc ttctctttta
    18061 aatattcggg gaatacgccc ttcctcggca cccgaacaaa tatattttac catgtgacgg
    18121 gaaattcata caagatatta ttttacattt tttgccatat tcgttatatt gcttgaaaaa
    18181 ttgcaaagtt tttgtcaatt attgcaaata ttttgagatt caaatgattt ctttttccaa
    18241 aaagaggcgg ttgcaagtca gacttacaat cgcctctttc attatagcct tattttcaca
    18301 acttatttat catccacgct gtagttcgga gcttccttgg tgatatggat atcatgagga
    18361 tggctctcct tcagggatgc tgcagagatc ttcacgaatc tgccggtctc cttcagagtc
    18421 tcgatatcct gtgcaccaca gtaacccata ccggagcgga gaccgcccag cagctggaat
    18481 acggtatctt ccacgctgcc cttgtaagcc acacggcctt caacaccttc gggaaccagt
    18541 ttcttcgcat tctcctggaa gtaacgatcc ttactgccgt tctccatggc tgcgatggaa
    18601 cccatgccac ggtatacctt gtattttcta ccctggaaca gctcgtattc tcccgggctc
    18661 tcctcacatc ctgcgaacat ggaacccatc atacatacgt taccaccggc tgcaatagcc
    18721 ttggtgatat ctccggaata cttaatacca ccatcagcaa tgatgggaat accatattcc
    18781 tttgccacgc tgtagcaatc catgatagcg gatacctgag gaacaccgat acctgcaacg
    18841 acacgggtgg tacagatgga accggggccg atacctacct taaccgcatc tgcgccggcc
    18901 tcgatcaggg cacgggtagc atcaccggta gccacgttac ctgcgattac ctgcagatcg
    18961 ggatattttt ccttgatcat cttcaggcag ttcaatacgt tctgggaatg accatgtgcg
    19021 gaatccagta caataacatc aaccttggct gctaccagag ctgccacacg atccagtacg
    19081 ttggcggtga tacctacacc ggcgccgcag agcagtctgc cctgggaatc cttggctgcc
    19141 agaggatatt tgatggtctt ctcaatatct ttgatggtga tcagaccctt taagttgaag
    19201 tccttatcta cgataggcag cttctctttt cttgccttgg ccaggatctt ctttgcctct
    19261 tccagagtga taccttcggg agcggtgatc aggccttcgc tggtcatgga ctctttgatc
    19321 ttcttggtga agtcggtctc gaacttcaga tcacggttgg tgatgatgcc gactaatttt
    19381 ctgccctcgg tgataggcac gccggagata cgaaacttag ccatcagagc atctgcatct
    19441 gccagagtat gttcaggaga cagagaaaac ggatcggtga tgacaccatt ctcggaacgc
    19501 tttaccttat ctacctcttc tgcctgtgcc tcgatagaca tattcttgtg aatgataccg
    19561 ataccaccct gtctagccat ggcaatcgcc atacgatgtt cggtaacagt atccattcct
    19621 gcgctcatca taggaatgtt cagcctgatc ttctttgtca gccatgtggt caaatccgcc
    19681 tgattaggga ctacctgaga gtaagacggt acgagcagta cgtcatcaaa ggtaatgcct
    19741 tcaccaataa tctgtcccat tttctttcct cctgtgttca tttgataaga tttgtgaatg
    19801 atatcttgtt gtgttataag ttgtgattat attacatatt cgttgtactt ataacatttc
    19861 catgagttta gcagagtttc gtaggactgt caaccggata tcttatattt tttatcattt
    19921 agcaatcata ttaccgcgtt tatttctgtt tctgcaataa agaaggtccg gcgtatcatg
    19981 tacaccagac ctatattttt gactcgccag gcattcaact gctttgatgc tgttccctgc
    20041 accggactgt tgcgaaatat tattcgctga acactttgcg gggtgccatg tttttgagca
    20101 ccttgaccag ctcctcatta gggcttaaga ggatcttttc gccaccttca tagtacatag
    20161 cataacgccc gtccggctgc aatacctcgc cgatactata atccttttcc ttcacctgac
    20221 ggtttttgaa gttatccaga tgataggagt aaaccggtgc aaatacctcc atgcggtcaa
    20281 ggctgtaagt accgacacgc tttctcttgc tcttagccat gatcttgtcc acggacagtt
    20341 ccttgtccag atagaggtat tcatattcca gatcggtata catattcagg aaataagctc
    20401 ccacccccgc tgccagtgcc accggcagga aaatgataaa cacgaacatt accagacagc
    20461 tcagtactgt cagtgcgatc aataatacct ttaaaatttt tgcccaggtg ggctgttttg
    20521 cttttaccag acattctacg taagaatcac tcattttttg tttccttttc tcttcgttta
    20581 ttttatgcag agcctattcc ctcttaagga aagccttctc ttctgggccg gttctgaaat
    20641 tgcccgcgcc gttcttccgt ttctttggga aatctctgtt tacgactgtc cggattctgt
    20701 tttctcctga caatcgtttt ctattatgat atatcatctt cacaaaactt gcaaggcatg
    20761 cccggatttt cccggcgatt taattgtttt tttagattcc cctcacactt cgttcattga
    20821 ctttcagata gcgaaccttt atcattagta tattatgaag aaatttaccc ggaatgtggc
    20881 gttctgccat atttcggcag cagaaaggcg gatctcccca tggcagtaat ggatgaattc
    20941 aaagaagaac gagaggcatt gaaaaacggt acgccaaggc agaaactggc gtatttctgg
    21001 tattattaca agtggcatgt gatcattgcc ctggtggtga tcattatgat catcagtttt
    21061 gtaaaacagc ttacagaccg gaaggatccc tctttttatg ccgtgatgct gaatgccagc
    21121 ctcttagatc agatgacctc ggaacagccg gattttgtta cggattttgc agaaaaagag
    21181 ggcattgatc tgaacaccag tgatatcact tttgatactt ccatccgtat cgtggagggc
    21241 agcatggatg agacttctat cacttcttcc cagaagctga tggtgtatgt ggcagccaat
    21301 gaactggact gtatgatcac agatttcact tctttccaga aatatgccaa cagcagcatg
    21361 tttcatgatc tgcgggatat tctcactgat gagcagatac aggcactgga accttatttc
    21421 tattatgtag acagagaagt ggtactggcc atcgaagcag ccaacgatga tatgaatacc
    21481 gattataccc cagactatcc cgatcccctc cacccggagg acatgcagga tcccgtaccg
    21541 gtaggcatct gcctcacaga ctgcaaggat ctgactgata cttattactt ccgtggtgac
    21601 ggcatcgcca tgggcatcta tgccaatgcg gaacatgtgc agaccgcggt ggatctggcg
    21661 gagtatctgt tgaataaata gaaagttagc
//
LOCUS       DAWEJW010000044        21470 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_969_length_21470_cov_46.793929, whole genome shotgun sequence.
ACCESSION   DAWEJW010000044 DAWEJW010000000
VERSION     DAWEJW010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 21470)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21470)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21470
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_969_length_21470_cov_46.793929"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3303..3402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5957 a         4881 c         5446 g         5086 t
ORIGIN      
        1 aacggacgtg gtattcccgt gggaatgcac gaaaaaggaa tcagtgcgga gcgcctggta
       61 ttcacgactc ttcatgccgg cggtaaattc gataattccg cctacaagac cagcggtggt
      121 ctgcacggcg taggttcctc tgtggtaaat gctctttcca caaagttgac tgtaagagtc
      181 aagagtggtg gcttcatcta tgaggaccgt tatgaaagag gtatccccgt gatggatctg
      241 gtggacggtc tgcttcccgt agtgggcaag accagagaga ccggtacgac catcaatttc
      301 ctgccggatg ataccatctt cgacaagacc cgtttcaagg aagatgatgt taagagccgt
      361 cttcatgaga ccgcttacct gaacccgaag cttacgatca tttttgaaga taaaagaaaa
      421 gaagagccgg agaaggtcac ctatcatgag ccggaaggta tcattggctt tataaaggat
      481 atgaaccatt ccaaggagac cgtacatgat gtcattttct tttccggcga gagcgaggga
      541 atctctgtgg aagtggcctt ccagtatgtg aatgaattcc acgagaatgt actgggcttc
      601 tgtaataaca tttacaactc tgaaggcggt acccacctta ctggctttaa gacccagttc
      661 accaatgtga tcaataccta tgccagagag ctgaacattt taaaggaaaa ggatgcaaac
      721 tttaccggtg ccgatgtccg gaacggaatg acggcagtgg tatccatcaa gcacccggat
      781 ccccgtttcg aaggccagac caagacgaaa ctggacaacc aggatgccgc caaggccgtg
      841 gccaaggtga ccggcgaaga gatcgtccgg ttcttcgacc gcaatctgga gactttgaag
      901 agcattatcg gctgtgcgga aaaggcagcc aagatccgta agacagagga aaaagccaag
      961 accaatatgc tgaccaagca gaagttctcc tttgattcca acggaaaact tgcaaactgc
     1021 gagtccagag atccctccaa gtgtgagatc ttcatcgtag agggtgactc tgcgggcggc
     1081 agtgccaaga ctgccagaaa ccgtatgtat caggccatcc ttccgatccg cggtaagatc
     1141 ctgaacgtag agaaagccag cattgacaaa gtactggcca acgccgaaat caagaccatg
     1201 atcaacgctt tcggctgcgg attttccgag ggctacggca acgatttcga tattagtaag
     1261 ctgcgctatg acaagatcat tatcatggcg gatgccgatg tggatggcgc acatatttcc
     1321 acgttgctgc tgaccttatt ctatcgtttc atgccggatc tgatcttcga aggccacgta
     1381 tacatagcaa tgcctccctt atataaggcc atgccctcca agggagaaga aaaatatctg
     1441 tatgatgata aggcactgga aaaataccgc aagacccaca agggtccctt taccttacag
     1501 cgatacaaag gtctgggtga gatggatgcc gaacagctgt gggagaccac actggatccg
     1561 gaacgcagat tgttgaaatt agtagagatc gaggatgccc gcatggcttc cgagatgacc
     1621 gagcttctca tgggtacgga tgtgccgccc agaaaagcct ttatttacga tcacgctacg
     1681 gatgcggcgc ttgatattta atatcagata tcagaagaac ggagattaga aatggacgac
     1741 agcagaatca taaaaacaga atactccgag gagatgcaga aatcgttcat cgactatgcc
     1801 atgagcgtta tcattgccag agccctgccg gatgtcagag acggcctgaa gccggtacag
     1861 agaagaactc tctacgatat gcatgaactg gggatccgtt acgaccgccc ctaccgtaaa
     1921 agtgcccgta tcgtaggtga taccatgggt aaatatcacc cccacggcga cagttccatc
     1981 tatgaggcac tggtagtcat gagccaggat ttcaaaaagg gcatgcccct gatcgacggc
     2041 catggtaact tcggcaacat tgaaggagat ggtgccgcag ccatgcgtta taccgaggca
     2101 agactgcaga aagtgaccca ggaagctttc ctggcagatc tggacaagga tgtggtagac
     2161 tttgttccga acttcgatga aacggaaaaa gagccgtccg tactgccggt aaagattccg
     2221 aacctgttgg ttaatggttc cgaaggtatc gccgtaggca tggccaccag cattccccct
     2281 cataatctcg gggaagtcat tgacgccgta aaggcttata tgctcaataa tgagatcaca
     2341 acaaaagaac tgatgcgtta cctgaagggg ccggattttc ccaccggcgg catcgttatc
     2401 aataaggatg aactggaaga catctatgag accggaaccg gcaagatcaa gctgcggggc
     2461 aaagtggaac tccagaaggg aaaatctgga aagaccaatg tagtgatcac cgagatcccc
     2521 tataccatga tcggtgccaa tattgcgaag tttttatccg acgtggccgc tctttccgag
     2581 acgaagaaga cacaggatat tgtggatatt tccaaccaat cttccaagga aggcatccgt
     2641 atcgtcatcg aacttcgtaa ggatgccgat cccgagaact ttgtaaatat gctctataaa
     2701 aagactcgtc tggaggatac cttcggtgtc aatatgctgg ctatcgcaaa cggcagacca
     2761 gagactatgg gcttaaagca gatcatcaag gccaatgttg acttccagtt tgaagtggct
     2821 accagaaaat ataccaatct attggccaaa gagcaggagc gcaaggagat tcaggaaggt
     2881 ctgatcaagg cctgcaatgt gatcgatctg gtcattgaaa ttttacgcgg atccaaggat
     2941 cgtgccatgg caaaagcctg tctcgtagag ggtaaggtcg atggcatcaa gttcaagtcc
     3001 aaggaatcca ggatcatggc agcccagctg atgttcaccg agaaacaggc caatgccatc
     3061 ttagagatgc gtctgtataa gttgatcggt ctggaactgg aagcactgat caacgaacac
     3121 gaagagacca tggcaaacat ctatcgttat gaggatattc tggaccgcag agattccatg
     3181 gcccaggtca tcatgaatga actggacgga ttcaagaagg aatacagcca tccccgcaaa
     3241 actgtcatcg agaacggaca ggaagcggtc tacaaggaaa aagaactgga agagatggat
     3301 gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaatgccgt agagaccacc
     3421 attgaatata agggcaagga acttgtcctg aacaatctga agcttggaaa gcgcgacagc
     3481 aagggcacca aggtcagagt ataacaaagt cagcttagaa aacggagaaa aatatatgag
     3541 agtcatagca ggaactgcaa gaagtctgcc gttaaagaca ccagagggaa tggataccag
     3601 acccactacc gaccggatca aggaaaccct ttttaatatg ctgcagacct atattccgga
     3661 ctgtgtgttc gtggatattt tcagtggcag cggcggcatc ggcatcgaag ccttaagccg
     3721 cggtgccaga aaagcctatt ttattgaaaa tgcgccgaaa gccctggcat gtatcgagga
     3781 caatctggca tttacgaaaa tgaaagaccg tgctatagta ttaaaacagg atgcctgcgc
     3841 aggactgacg ggcatctatg aaaagcacgt agatgtgatc ttcatggatc caccctacga
     3901 tcaggagcag gagcgcagag tcttagagca gctgcgctat gccaaatatg tctccgaaga
     3961 gactctgatc ctggtagaag cctctctgca taccgatttc tcctatgcgg aagacttagg
     4021 gtttgaagtg atccgtgaga aaaaatacaa aaccaacaag catgtgttcc ttcgtaagaa
     4081 gtaacactga aaggcatgat ttttatgaaa agagctgttt acaccggaag ctttgatccc
     4141 gtcaccaacg gacatatgga tatcataagg cgggcatccg aaatatttga tgttctgatc
     4201 gtcagcattt tgaacaataa agaaaagaca ccgttgtttt ctgtagagga acgtgttaag
     4261 atactagaag aagccacaaa agatctgcct aatgtgcaga ttgacagttt cagtgggctt
     4321 ttagtggatt atgccaggga aaaagatctg catgttatcg taaggggctt acgcgccatt
     4381 acggattttg aatatgaact gcagatggca cagaccaacc ggatgttgtc ccatggcaaa
     4441 attgatacgg tatttttgac caccagtctg gaatatgcct acctcagttc ttccgccatc
     4501 aagcagattg caagttttga tggagatatc accccttgtg ttccggattt tgtggcgaaa
     4561 ctgatctacg acaaatatcg cgagaaaaag ctttcagagc atagtgatgt gtaaataagg
     4621 agagtttcat gagcagcaga atcgaacagt taattgatga aatagaagaa tacatcgagg
     4681 gttgcaagcc gaagttcatg tccagtaccg aaatcatcgt gaataaggat gagattgatg
     4741 agttactgcg ggaactgcgt atgaagaccc cggatgagat caagcgctac cagaagatca
     4801 tcagcaacaa ggaagccatc ctaaacgatg ccaaagccaa ggcagaagca ttgatcaacg
     4861 aggctaccgt gcataccaat gagctgatca acgagcatga gatcatgcag caggcttacg
     4921 cccaggccaa cgaggtcgtt accatggcca gtaagcaggc ccaggagatt ctggacaatg
     4981 ccactattga ggccaacaat gtcaaatccg ctgccatgca gtacatggat gatatgctgg
     5041 cacatctgga gaatattatt tcttcctcca cacagcaggc cagcgccaat tacgatgctc
     5101 tgatcggcaa tctgaaccag tatcgtgaca ttatccagtc caaccgcagt gagctgcacc
     5161 ccgtagatga ggatcttgat gacgtagata ctgctgcgga tgccgaagac aatctggacg
     5221 ttatgtaaag catgtccaaa caacgagtac gaaaaaccag ttttctgctg aaagctggtt
     5281 ttttaaaaat aaaggaatac gataaatgaa agtatggaaa aagcaaaaag ggctgttagt
     5341 caggacaatg atctgtgcgg tcagtcttgc ggcagcctgt ttttatggaa attccctgcc
     5401 tgcacaggcg gctcccagag cgcaggagac cggagaaagg gtcgtcattg tcattgatcc
     5461 cggacacggc ggtgataacg agggaacgct ggaaggccct gcacaggaga aaaaaatgac
     5521 catggtgacc gcgctggcca tgtacgagga actgacaaaa tacgataatg tggatgtcta
     5581 tctgactcat acggatgatg tgaccatgtc tctggcagac cgggcacaat ttgctgcaga
     5641 ccggaatgcg gatttcctgt tcagtattca ttacaacgct tccatggatc atgatctgtt
     5701 cggatccgaa gtctggatct cttccgtaca gccttacaac gcttacggtt atcagttcgg
     5761 ctgggaacag atgcagcaga tgcaggacat gggactgttt ctacggggag tcaagacgaa
     5821 actgaaagat aacggtgatg actattacgg aattataaga gaaagtacct caagatccat
     5881 tccctccgcc atcatcgagc attgtcatgt ggatgagagc agagacactc cttactgcca
     5941 gacagaagag gactggaaga aattcggcag ggaggatgct ttgtctgttg caaaatattt
     6001 cggattatcc agtgcaagtc ttggtgtgga ctacagtgga gaggcagacg ctcttccgga
     6061 agtctcgctg caatccattc tgcctgccac tgtgcgggac aagaccgaac cggatatctg
     6121 ccagctgact ttgcagaatg cagactatga gaccggagaa gtctctctgg aagtgacggc
     6181 agccgactat gactgcccca tgatgtatta cgattacagt accgatggag gcaggactta
     6241 ttccgaactg cttccctggc cgggactgga tattatggcc gggacctatc ccgatacctt
     6301 cacattttcc gtgaccttca caaaggggga acagccggtg atcaccgtcc gcggttacaa
     6361 tcaggcggat ctgttcacag agagtgatcc tgtcaccttt gcacagcctt ttattgatca
     6421 gaaaaaagct gccgaagagg cattacagga aagtcttgcg gcacaggagg cagcagaagc
     6481 ttcccgggct gcagaggaac agatcacaga agctggcagt tccgtgatca ccatggccga
     6541 tgccgccgga aatactccgg tgccttccga aaaaagtccg gaagtgaatt tcggtgtttt
     6601 tctggtgatc tgtgggattc tggtagtgct gttgttactg gtctttttgt tataccagat
     6661 ccaacagagc agacacagaa aacgaagacg gaaaaatcaa agcagaaaag tatccggaga
     6721 caacagaaac catcccagat aataagctgc agtaagcact gtcagtacca ttttatgaag
     6781 gatgtaggac ttgagggaaa gccctgtatt ccggatacag cttccggtct gggcaataca
     6841 gcttaagcca ccaaagggca aaagcaatag tgcatacaaa ggacatttgt ctccaagaat
     6901 tttcagaccc ccactgatct cccaaagcgg tgcatagcgg aagctgtcgg aaaccagaag
     6961 ccttggcaga aggttcagca gattgaaaaa gatcatgtag cctcccagct gtaagatgct
     7021 tcttcctgct gctgtaatgg catcatccag ggcatccggc agggacatgg caggaaggca
     7081 ggccggatcg gtctgttcta aaagcttcgc tgcgttgtct cttccgaaag cttggtctgt
     7141 tttctctgga atccgatttc tgtatacgct ataacgcagg gaaataccat atagcaatgg
     7201 aatgccatac ataccaaata cataggggag caccagcttc ctatgcagta gtggcagtac
     7261 aaaacccaga aaatagaccg gtcccagatt gttgcagaag gccagcagat attgtccttc
     7321 ctccgaagaa atttcttgtc tgtcccgaaa atccgaggca gtgcgggcac ccatgggaaa
     7381 accgcacaaa aagcccacca ggatcacata gattcccggt gctgacaggc gatagattct
     7441 tccgaacaaa ggtcttaccg gacggatcag gctgtccgtc atgcccatac ggatcaaagt
     7501 gccggacagg atcataaacg gcagaagcac cggcaccatt ttttcgtacc aaagacttaa
     7561 tcccagggca gcataggcaa ggctcaggga agaatgagtc agaatacagc cggtcattag
     7621 aacaaagaaa cagattccga atattttttt catggctgtc tgccttgaaa ggtttgtata
     7681 ctatatgcca atgaagaaaa attaagtcta agaaagagat caaagcccat gcggttggat
     7741 aaatttttat gcgataacaa tattggcacc cgaagtcagg tgaaagagta catcaaaaaa
     7801 ggtcaggtga cagtaaacga ccagataatc aaaaagccgg agacaaaagt caatgaagag
     7861 accgatacgg tggtctgtca gggacagccg atccattata ggaaatatgt ctattatatg
     7921 ttaaataagc cagagggggt tgtctctgcc accaacgata atacagcccc aaccgtggta
     7981 agtctcctta ctgtcccgga acaaaaggat ctgttcccgg tagggcggct ggacaaggat
     8041 accacgggat tgctgctcct gaccaatgac ggtgctctgg cccatgatct gttgtcaccg
     8101 aaaaaacatg tggataagac ctatctggta aagccggaaa agccgctttc cgcagaagat
     8161 atccatcggt tggaaaacgg gcttgacatc ggtgatgaga aaccgacagc cccgtccaaa
     8221 gccagactca cagaaaacgg tgatctgtta ttgaccattc atgaaggccg gtttcatcag
     8281 gtaaaacgga tgttgcaggc ggttgataat caggtactta cactggagcg ggtaagcttc
     8341 ggccccctgt ccctggatct ttccctgcaa agaggggaat accggacatt gacggaagaa
     8401 gagatcgaag tcttgtctgg cgctggaaag aaatcataaa taaaggagtt atttttcatg
     8461 caattcaaaa gaatgtttac cactgatgcc tatagaggaa cccgcgggta tctccgcaca
     8521 cagaaaattt atgagatttt aagaaccgtg ctttattttg ccatctctct gtctttgttt
     8581 atcgccggat gggtcagcac cggaagccgt gagaatctcc tgaccatcgt ggcggtactg
     8641 ggatgtcttc cggcctgcaa gagtctggta gaaatgttca tgttcctgcg ctaccggggc
     8701 tgtgacgagc aggtagctgc aaaaatcgca gcacatacag aaggccttac cactctctac
     8761 gatatggtat ttacttccta tgagaagaat tatgttatct atcatctgac agtctgcggt
     8821 aatacagtct gtggctacac cacggatccg aaatttgcgg aacaggcttt ttacaagcac
     8881 attcaggaca ttctgaaaaa agacaattac agagaagtct ctgtgaaaat atttaacgat
     8941 ctggacaagt atctggcaag atgtgatcag ttaaaggagc tttcctcaca gccggaactg
     9001 tctgccggga tctgccagac cttaaagagc gtatctctat aacttacttc tcaaaaggag
     9061 ggtgtcctat gcagttgctt accattggca aaaatcaggc gggacaacgt ctggacaaat
     9121 ttttaaaaaa agcgttacct aatgcaggca ccggattttt atataagatg cttcgcaaga
     9181 aaaatattac gttgaacggg aaaaaggctg aaggtaaaga aatccttgcc cagggagacg
     9241 aggtaaaatg ctttttctct gaggaaactt atgcctctct gtccggtgcc ggggcagcgg
     9301 aagatacttc cgattaccgt aaggcttacc gttctttaaa ggatatcagc gttctttatg
     9361 aggatgaaaa tatcctgttg ctgaataaac ctgccggagt tctgacccag aaagccaaac
     9421 cggaagatct ttccctgaac gaatggctga ttggccacct gttggctgcc aatgccataa
     9481 aagaaaccga tcttgccacc ttccatccat cggtctgcaa ccgcctcgac cgcaatacca
     9541 gcggtatcgt attgtgcgga aagacgctgg ccggttccca ggcactcagc cggatcatca
     9601 aggatcgttc cgtaaagaaa tattatcaga ccgtctgtaa gggcaagatt ttacaggaaa
     9661 gcaccctgga aggctatctg tacaaggacg aacgcaccaa tacggtacag gtatttccag
     9721 atatggcgga ggctccggaa gatgcttcct ttataaaaac aatctacact cccagtgcgg
     9781 tggctgggga atatacccta ctgaccgtag aactggtcac cggcaaaacc caccagatcc
     9841 gcgcccatct ggccagcacc ggacatccgt tacttggtga caccaagtac ggagacaaca
     9901 aattaaatcg caggatgcaa tcggaatact ctctgcacca ccagcttctg catgccggaa
     9961 gagtgtgttt cccggaagaa gcggaaggac ctcttgcaaa ggtgagcggt cagactttca
    10021 acgcaccgct tccccataaa tttgcagaga tcttacaggc tctgaatctg acaccggatt
    10081 ccgcctgcta gaaaggaaaa ctaagaacaa tggcaacatg gaataccaga gggcttcgtg
    10141 gatctactct ggaggatatg gtcaaccgca ccaatgaaaa atatgccgag gccggtctgg
    10201 ccctgattca gaagatcccc acccccatta ccccggtcaa aatggataag gagagccgcc
    10261 agatcaccct ggcctttttc gaacagaaga gtaccgtaga ctatatcggt gtcgtacagg
    10321 gcattccggt ctgttttgat gccaaggaat gtgcggtgga caccttttcc ctgcaaaata
    10381 tccatcccca tcaggtggag ttcatgcatc agttcgagaa acaggggggt atcgcctttt
    10441 tcctgatatt ttacagccat aaggatctgt tatattacct tccctatgaa atgctccgct
    10501 ttttctggga cagggcacag gagggcggcc ggaaaagctt ccgtttcgag gaactgaacc
    10561 cggaatatat tatcccgaag catcagggaa tcctggttcc ctatctggat attctgaaaa
    10621 aggatctgga agaccgggag gaataataaa taataatttc ttgacagcgg agaggtctcc
    10681 tagtatacta caaatagttt cgcgtgctac tatgataata tagtatcacg ttaatatatt
    10741 aaagtgtatg aattgaggaa cgcttatgaa caacagactt ttacatacgc cagagggcgt
    10801 ccgggacatt tacggcaaag aatatgcaaa gaagctggct atagaacaga aactgcagca
    10861 ggcgatccat tgtttcggtt acgaagatat ccagacccct tccattgagt ttttcgatgt
    10921 tttctccaag gagatcggaa ccactcccag taaggagctg tataagttct ttgataaaga
    10981 gggtaatacg ctggtgctgc ggccagactt tacgccgtcc atcgcccgct gcgcagcgaa
    11041 gtattttatg gaggaaaagg tgccgcttcg tttcagttac aacggcaata cgtttacaaa
    11101 cacttccaat ctgcagggta aattaaaaga ggtgacccag atgggtgcag aactcatcgg
    11161 agatccttcc gtagaggcgg atgccgaagt aatcagtatg gtgatcgaag ctctgaaaag
    11221 cgcaggactg accagattcc aggtgactat cggtgaggtg gattatttcc gcggcctctg
    11281 tgaggaagcc ggtctggatg aggatacgga aaaggaactt cgttcctgta tctccggcaa
    11341 gaactttttc gctgcccagg aattactgga gcagaagcag atccccgagc cttatcacag
    11401 cattctgtta aagctggcgg atatgttcga aaatatagag tctctggagg ctgccaaagc
    11461 actggttaaa aatgaaagat ccttacaggc gattgcaaga ctggaaaagt taaaagagcg
    11521 cttagagcag tacggtgtgg cggaatacat ttcctttgat ctcggaatgc tcagcaaata
    11581 tcagtattat accggcgttg tcttcaaggc ttacacctac ggtatcggtg atgccgtggt
    11641 aaagggtggc cgttacgaca atctgcttcg gaaatttggc aagaatgccg cagctatcgg
    11701 tttcgtcatc gtcattgacg acctgttgga agcattatcc cgccagaaag tgaatatcga
    11761 tattcccgct tccggcaaaa ccgtgtatta ccgggcagga gacgaggcag attatcttgc
    11821 gaagctttcc gaagtcataa ccctgaggaa agaagggatt cccaccgtgc tttcccccga
    11881 gatcacagag aacgaggagg cagatcagta atgagtgaag aaagatatct tacgtttgct
    11941 ctgggaaagg gcagacttgc caaaaagaca ctggagcttt tcgaacagat cggcattacc
    12001 tgtgaagaaa tgaaggataa agataccaga aagctgatct tcgtcaatga agaatacaaa
    12061 ttacgctttt tcctggcaaa gggtcccgac gttcccacct atgtagagta tggtgctgct
    12121 gatatcggcg tggtcggcaa ggacaccatt ctggaagaaa accggaatgt ctacgaagtg
    12181 ctggaccttg gcttcggcaa atgccggatg tgtgtctgcg gtcctgcttc cgccagagag
    12241 ttgttgaaac accatgagcg gatccgggta gccagcaaat accccaatat cgcccgagag
    12301 tatttctata ataaaaagca tcagaccgta gatatcatca aattaaacgg ttctgtagaa
    12361 ttagggccgc tggtagagct gtctgacgtc atcgtggata tcgttgagac cggttccacc
    12421 ttaagggaaa acgggctgga ggttctggaa gaggtatgtc ctctgtccgc ccgtatgatc
    12481 gtcaatccgg tgagtatgca gatggaagcc gaccgtatcc gcaagctggt gggtgcgatc
    12541 cgggagcagt taaaaataca ggcctgaccg agacgataag catcgtcata aaaagtattt
    12601 gaaggagagt accgtcatga gaatcgtagc attggataaa gaatcaaagc agagtatttt
    12661 gaacgacctt ttaaagagaa gtccgaacaa ttattcccag tatgagagca ctgtaaacca
    12721 gatcatcgaa aaagtccgtg ccgagggtga caaggcattg ttcgattata ccttacagtt
    12781 tgataagttc gctttaagcg cagagaatat ccgtgtcaca aaggaagaga tcgccgaagc
    12841 ctatgcacag atggatgaga atctgatcga tgtgatcaga cagtctgcgg aaaacatccg
    12901 cgcttttcac cagaagcagc ttcgtaacag ctggttcgat gccaagcccg acggtacgat
    12961 cttaggcatg aagatcaccg ccatcgaaag agccggtgtc tatgtccccg gcggtaaagc
    13021 cgcttatcct tccagcgtac tgatgaatgt gatccccgcc aaggtagccg gtgtggatga
    13081 gatcattatg accacccctc ccggaaaaga cggtaaggtc aatcccggaa ctctggtagc
    13141 cgctgatatt gcaggcgtgg ataccatcta taaggtcggc ggtgcccagg ctatcgcagc
    13201 catggctttc ggaacccagt cggtacctaa ggtcgacaag atcaccggtc ccggtaacat
    13261 tttcgtagct cttgccaaaa aagctgttta tggctatgtc agcattgatt ccattgcggg
    13321 tcccagcgag atcctggtac tggcagatga gaccgctacc cccagatacg ttgcagcaga
    13381 tctgctgtct caggcagagc atgacgaact ggcaagtgcc attctgatta ccacttcgaa
    13441 ggaactggcg gagaaagtct ccgaggaagt ggacggcttc gtaaaagtac tggagcgtgc
    13501 tcccattatc cagaagtctc tggacaatta cggctacatt ctgttagccg acaacatgga
    13561 agatgctatt gatgccgcca acgccattgc ttccgagcat ctggagatca ttacaaagaa
    13621 tccttttgat accatgaccc gtatccgcaa tgccggtgcg attttcctgg gagagtatgc
    13681 ttccgaaccc ttgggtgatt attttgcagg tccgaaccac attctgccca ccaacggtac
    13741 cgcacggttc ttctccccgg taaacgtgga cgacttcatc aagaagacca gtattatctc
    13801 ttattccagg caggcactgg aaaaggtgca caaccagatt gagaccttcg ccaagagtga
    13861 aggcttgacc gcacatgcaa acagtattaa agttagattt gaagattaat catttcctct
    13921 gggaagggag tcatcggata ttatggaacg aattgcaact gtgacccgta caacgaaaga
    13981 gaccgacatt gccatcacct taaatctgga cggcaccgga aaagcggatc tgtctaccgg
    14041 catcggattt tttgaccata tgctggacgg ctttgcgagg cacggctttt ttgacctgga
    14101 atgcaaggtg aagggcgatc tgcaggtgga cggtcaccac acggtggagg ataccggcat
    14161 tgtcttcggt gaagctatca aaaaagcggt aggagacaag aagggcatca atcgttacgg
    14221 ctattttatt ctgcctatgg atgaggtact tgctttgtgc gccgttgact taagcggccg
    14281 gccttatctt aattttgaat gtgaatttac tgtaccgaag gtaggtgaac tggatacgga
    14341 gctggtaaag gaatttttct atgccgtatc ctacagtgcc ggaatgaact tacatatcaa
    14401 aatgttgtct ccgggcaata atcaccatat gatcgaagcc atgttcaagg catttgccaa
    14461 ggcgctggat caggccgttt cctataatcc gaaggtaacg gatgttttaa gtaccaaagg
    14521 aagtctgtaa aaaatacgtg cctgcggcat agactttgca ggtatagaaa gacgtgagga
    14581 ttactatgat catcaagaaa tttgttccct gtatttattt atatcatgag catgcggtgc
    14641 ggaatcttac ggataccacc atcgtggata cggatcccgt ccgtctggca gattattact
    14701 gtgagcacaa tgcggatgag ctgatcgttt tcgatatgtc cgaaggtgat gcggagcatg
    14761 atgccgcact ggatatcatc aaggagatct gtaccagggc ggaagtggat gtcatcggtg
    14821 ccggcaatgt caagcgtatg gaggacatta agaaattact ttatgccgga tgtaaaaaag
    14881 cagttctcga ctatgagaaa gagagtaata ttgagattac cgaagaggtc tctctgaaat
    14941 tcggtaagga caagattctg atctcttaca acgacccgtc cgttctggaa ttacataagg
    15001 agaagattga gaaatatatc tctgccatga tcctgatgaa ccctcatcag atccgggaga
    15061 ctcaggccat tctttccctt ccgtttttcg tgcagatcaa tcaggtggca ttgaataaat
    15121 tgctggagat ttttgcttat gagaatgtct gtggtgtcac cggcaatacg ataaatgaca
    15181 atgtgaaaga gatcgtagcc ctgaaggatc tgtgccggga gaacgatatt cccattgaat
    15241 ccttccaggc tgcctacaag tgggaagatt tcaaaaagaa cagtgacggc atggtacccg
    15301 ttatcgcaca ggattaccgg acacaggaag ttctgatgat ggcttatatg aatgaggaag
    15361 cctatgagca gaccctgaag ctgggcaaga tgacctacta cagcagaagc cgtcaggaat
    15421 tatggctgaa gggcctgacc agcggtcatt accagtatgt gaaggaactg gtggcggact
    15481 gtgatatgga tacaatcctt gcgaaggtat ctcaagtggg cgctgcctgc cataccggaa
    15541 gcagaagctg cttcttcaat gagatcacga aaaaggatta tgaggaaagc aacaatccct
    15601 tacaggtatt cgaggaagtc ttcgatgtga tcaaggaccg caaggtccac cccaaggagg
    15661 ggtcctatac caattacctg ttcgacaagg gcattgacaa gattctcaag aaactgggcg
    15721 aggaggcaac tgagatcgtg atcgctgcca agaatcctaa ccccaacgag gtcaaatacg
    15781 agatcagcga tttcctttat cacatgatgg tgctgatggc ggaaaaggat gtcacctggg
    15841 aagagatcac tacagagctt gcgaaccgtt aactgcagtt tccacccagg tctccagata
    15901 agtgccgtaa tctacatcgg tgacataatg cagcacctga tccatggaga tcccggcaaa
    15961 gcatttgcct ttttgatcca gcaatatgat cgtcaataat aatactccgg ccagagcaag
    16021 gcggattttg aacgtaccgg tatcggaaga tgcggcaggt tccgaatcgt tttgctctgg
    16081 ccgtatttct gcctgtctgc gcatctgctg taagagttct tccttatgtc tgtgatcatg
    16141 tttttccatg ctcttttaca ttcctttctg atctctgcaa tcttcgcagt tcatatttat
    16201 gtgccttttg gtactggcag aacttaattt tataaggtat aatgataact attcagagcc
    16261 atgtaagcac ttcataagca aaaaggtaac tctgaaagag ttttttgcga ataaagttct
    16321 gaatagtttt agtaatttag gagaaattat ggctgaacaa aacggaaaag aattagcatc
    16381 cagaatacaa aacaatattc aaaaagtcct ggtcggaaaa gaggaagtga cagaacttct
    16441 tctgacggcc ctaatcgctg acggacatgt actattagag gatgtccccg gcactggcaa
    16501 aacgaagctg gccaaggcac tggcaaaatc tttaaatgcc cggttttccc gggtgcagtt
    16561 taccccggat ctgttgcccg gtgacattac cggcatcaat gtctatgaca gacagaagaa
    16621 tgaattcacc ctgcgaaagg ggccggtatt taccaatatt ttactggcag acgaattgaa
    16681 ccgtgccacc cccagaaccc aggcaggact gttggaatgt atggaagagc gtcaggtaac
    16741 catcgagggt gtctcttata caccgggcga acccttcttt gtcattgcca cagagaatcc
    16801 catcgaaagt gccggcgtat ttcctctgcc ggaagcgcag ttagaccgtt tccttataaa
    16861 actgtccatg aaccttccca ccaaagaaga ggaactgagg attctggaat tgtacatgga
    16921 ggaagatccc ctgaaaagcc tgactgccgt ggtctctctg gaggaacttc tggctgcccg
    16981 ggcagaggct gcgaagattt ttgttcatcc ctgcatccgg gaatatatgg tacagattgc
    17041 agaagccacc agacgggcag acggtattct ggcaggcgtc agtcccagag gaacccttgg
    17101 gcttttacgc tgtgtcaagg cctatgctta tctgcaggga cgttcctatg tgatcccgga
    17161 tgacgttcgt accctggctg ttccggtgct ggcccacaga ctggtatgca gcttcggttc
    17221 cgataccggc tccgcagcag gcagaatgga aaacatcctt tccactgttc ctgttcccac
    17281 agaaaggttt gagccatgat acaactgctg ggaatcattg tctttgccct gcttctgtat
    17341 ctggggcaga aatatgtata cgccaaaata tggcagcggg atctgaaggt ttccctttct
    17401 ttccgggagg atggcatctg ggaaggacag gagagcgagc tgtcggagat cattgagaat
    17461 cgcaaacggc tgccccttac catgttgaag gtcaaattcc agactgacag acatctggtg
    17521 tttgccgata ccaaaggctc ccggaccacg gatcgctatt accgcaatga tattttccag
    17581 atcggacgtc tggagaaggt caccagagcg ctgcacttta ccggcggaag aagaggttat
    17641 tacaccatac aggaggcgga tcttgtcgcg tccgatctgt tcctgacagc acagtatacg
    17701 gctacgacac ccatcgaaca ctgcagtctc tatgtgtacc cgaagccctt ttcccatccg
    17761 gattttaaac ggtctttaaa acagttaaac ggagaagttc tgacaaaacg acatcttttg
    17821 gaagaccctt tcgaataccg gggcattcgg gattaccaac cacaggatga cttaaaaagc
    17881 atcaactgga aagccacggc ccgtaccggg gatctgaagg taaaccagaa aaattatacc
    17941 tcccagaaat ccgttcggat ttttgtgaac ttagaggaca ccggtattct gaaaaaagag
    18001 gactgcgtgg aagcctgcct gcagattgcc acggcactgt tattgctgtt tctggaacag
    18061 gggatgcagg ttgccctgta ttgcaacggt attgacacaa tcagtggaga cccctgtatt
    18121 ctggaagccg gcggcggagt cagacagcgg aatgtctgtc tgcagacgct tgcaagaatc
    18181 gatacggcta aaccggtaca gtctttttcc gagctgtttg ccgcccgcat gtccgatgcg
    18241 tccaatgcgc tctatacctg ctttgtatca cccaatgcct atgaagactt tgcagcatta
    18301 cttctgcaat atcaggaaaa acacccggat atgaaatggt tttatcctta ttccgaaagt
    18361 acacctcctg atcccgggga agcgttaaag tctttgatca cttttatctc cctacgggaa
    18421 gaaagcgtct gaatgtgaaa gaaattttga ctgatctaat gaatcaatta tgtttttatc
    18481 ccctggcgct gactgcgtcc acgttgttgg ccgcgctggc tccggcgagc cgtccgtcct
    18541 tggctttgtg gatgctcggg gcgctgccgg tgttttttct gtattacgcc agaaaacgct
    18601 gtaagcatct gtcggtattg ctccttctgc acggactggt actggcatgt ttttatatca
    18661 ttccggcagc caacggcgta aaccgtggta tccggctgtt ggtaggtgca ggctttgtga
    18721 tctattccct ctggctgcgt tttcggacgg aggactttgc ttccgatccg ctgcctctgc
    18781 cgttagccgc cggaattgcc tttttgtgtc tttttctgca gcattatcag ggaaacaacg
    18841 tctgggatct ctattatcgt atgagtctga tcctggtgtt tttattgtat gctgtcattt
    18901 tgtttttgca gtcctatgag gatttcctta cggtaaacag gctgagtacc ggcaagatcc
    18961 cgtttcagga gatctttcgt tccgggctcc acagcaccgc aaccttcgtg ctgttatcgg
    19021 ggcttctgct cctggcagtg tcacagtttg cctggttaaa gccctttttg cggttactgc
    19081 gaaacggtct ggctgcgatt cttcgtttcc tgttcggact gcttcccatg gattccggga
    19141 cagaagcggt agtggtggaa cagcaggcag gcggtggcga tatgcctccc atggaagcgg
    19201 aagatccttt tattttatgg gtcattctgg aatatgtggc catgatcgca cttctgatcg
    19261 tctgcgtatt tctgctgtac cgcggaattc gaaagctgat cacatttctc agggacagaa
    19321 tgcagttttc cctccccggc actgtttctg aacagccgga gagcatggac aagcgggaac
    19381 acctggaggc tgccccggaa aatactgccg caaagcagaa gcagaaatgg ttttcttttg
    19441 ctttcttaga tgccggccag aagataaggc ggatgtatca gaaaaaaata aatgccagcg
    19501 ggcttgcaaa gatgagcctg ccattttata ctgctagaga tgccgaaaaa tcgctggatg
    19561 tggaaggaat ggctgaaatc tatgaaaaag cccgttattc cgatgtcccc tgcacagacg
    19621 gagatgtccg gcagatgaaa aattatatgt aaaaatcaat aacctgaggt aaatatgaac
    19681 gaaaagttag cgttaaatga tgatattctg atgaagatcg aaaaaccggc ccgctacatc
    19741 ggcaacgaag tcaatgccgt agtgaaggac agggacagca ttgatgtccg tttctgtatg
    19801 tgcttcccgg atgtctacga gatcggtatg tcccacctgg gaatccagat cctgtatggc
    19861 atgctcaatt cctgggatga tgtctggtgt gagcgtgtgt attctccctg ggtggatctg
    19921 gatgaaatta tgcgtaagga gaacattccg ctgtttgccc tggagagtca ggatccgata
    19981 aaagacttcg actttttggg aatcaccatt cagtacgaaa tgtgttatac gaatattctg
    20041 caggttctgg atctggcggg gatcccgttg cttgccaccg aacgtggaga ggactgtccc
    20101 atcgttatcg gcggcggtcc ctgcacttat aatcctgagc ccattgccga tttctttgat
    20161 attttctata tcggagaagg cgagacccag taccggccgc tgatcgatct ctataagaaa
    20221 tgccggacgg agaagacagg cagagaagaa ttcttacgcc gggcggcaaa gctgccggga
    20281 atgtatgtgc cccggttcta tgagaccact taccatgaaa atggcaccat tgcttccttt
    20341 cgacctacgg aagagggaat tcccgcaagg atccgcaaag agctggtaac ggatatgacc
    20401 gacagctttt atcctatgaa gccggtagtt ccttatatca aagtcacaca ggacagagtc
    20461 gttctggaga ttcagagagg ctgtatccgc ggctgtcgtt tctgtcaggc aggacagctg
    20521 tatcgtcccg tcagagaaag ggatgtggaa gttctgaaaa agtacgccat ggaaatgttg
    20581 aaaaacaccg gtcatgagga gatttcctta agttccttaa gttccagcga ttacagcaag
    20641 ctgccggagc tgattgactt cctgattgaa gagtgtgata agagacatat caatatctcc
    20701 ctgccgtctt tacgtatcga tgccttttct ctggatgtca tgagcaaagt acaggatgtg
    20761 aagaaatcca gtctgacctt tgcgccggaa gccggcagcc agagactgcg ggatgtcatc
    20821 aataaaggac tcactgaaga agtgatctta cagggttccg cccttgcctt cgaaggcggc
    20881 tggaccagag tcaaactgta cttcatgctg ggacatccca cggaaaccga agaggatatc
    20941 cggggaatcg ctgagctttc caacaaaatt gcggccactt tcttcgatac ggtgccgaag
    21001 gagaaacgtg tcaacggcag agtgcagatt gttaccagta cctccttctt cgtgccgaag
    21061 ccctttacgc cattccagtg ggcgccccag tgtaccaagg aagagttcgt ggaaaaggca
    21121 tatctgacca gaaaggccat ttccgagcag ctgaatcaga agagcatcaa gtacaactgg
    21181 cacgaagcgg atgtcagtgt tctggagggt gttctggcca gaggtgacag aaaattgtcc
    21241 caggtgcttt tatatgtcta taacaagggc tgcttctacg atgcctggag tgagtatttc
    21301 cacaacgatg tctggatgga agcttttgaa gcctgtggtc tggacccgga tttctattcc
    21361 cacagagaac ggcctctgga cgagatcctg ccctgggatt tcctggactg cggtgtatcc
    21421 agagcctttc tggagagaga atggcagaaa gcgaaaaacg agacgatctc
//
LOCUS       DAWEJW010000045        21423 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_971_length_21423_cov_57.292119, whole genome shotgun sequence.
ACCESSION   DAWEJW010000045 DAWEJW010000000
VERSION     DAWEJW010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 21423)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21423)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21423
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_971_length_21423_cov_57.292119"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    17306..17405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6023 a         4520 c         5960 g         4820 t
ORIGIN      
        1 tattcatata tgaaaagaaa tgaaaaatac cttttgtaaa atcaatctgt acaatgggag
       61 aatgtgttac atgccttatg ataaagcaga gcaagaacag aattccaatc gcaaaagccc
      121 catgatcgat ccatttgtcc gataagtgaa gaagccggaa tttccaaaga ggaaaaataa
      181 taaaaattcc caatactaca ggccagagag gatgataata aaaggctgtt gtaagatcac
      241 cgcttaatag gcataaaaat gctctggtca ttccacagaa agggcatgga atgccaaaaa
      301 agaatagtaa agggcattga tagcctgaga accacaggaa aagcaggagt agtgcaataa
      361 tagaaataag aaatatagtt tgttttatgt ttttattcat gatcagcaac ctataaaaag
      421 aaaaaagctt ttgtcgtctc cggcaaaagc tttttttatc ctgtgattat gctgtatatt
      481 taccgtctac gaatacaact tcttcggaac cggcataagc agcaccgtat gccttggtaa
      541 gagcaatgat ccagtcgacc aggctccata ctccgcagcc accggcagtg agaagcttca
      601 aaattccgag accaacctga cctctgacga atctatcaac acccaactca ccaagtaaaa
      661 agcagaaaac ccaggcaaat acatgcttgt taacacgatt ttccattaga aagcccctcc
      721 tttgtattaa atgatatcat cattatgatc cgttagtgag aaaaaatcaa tcaataaacg
      781 aaaaaaacat aaaattatgt gctttaaggg tctataaata gttattagag gaacgtttta
      841 aaataaagcg cacattgtat ccggattcgt cacagatccc gcggacaatc tcttccgcag
      901 catccttgac attgtcgttg atgcagatga tgcaggcatc ttttttgcgg ctgaagaagg
      961 aagccttcgg ccagcggata taataagaaa tccggttttt cagcaatgct ctctcgatga
     1021 gctgcttgca atcgggatcg gatacttcgc acaattcaat ctcattatta actttcagag
     1081 tggtatatag cggctgcatg atgtcacctc caatagtatt acaaggcgat tatagcacag
     1141 attctttgaa atgcgcaagt agcgcttata cttgacgcgg tatccggaag aaggtacaat
     1201 aatacacacg gaatatggaa accgaaacag cccggacggg aaggacagac agatccaaag
     1261 tgcgggcggt aggaagagaa aggagatcat acgatggata ttatagagaa gatcggtgat
     1321 accatcaccg aaaagggaaa agtagcggca gacaaagcaa aagaactggc agccattgct
     1381 aatctgaaga gccagatctc tacctgcgag gaagtcatga agaagaacta tctggagatc
     1441 ggcaagatct attttgaaca gtacagcgat atgccggagc ctccttttga gaagcagtgc
     1501 gagaccatta agaacagcca gagagccatt gcagatttac aggaaaagat cgaacagctg
     1561 aaaacaaaat aaaacgaaat gacgaaaata aaaacagggt tgacagcaat cctgttttta
     1621 tttataatgc agacataaca acgttatgtg aacgagtgaa catatgatag aataaactct
     1681 tccatagaaa acatacctga ctgcttgcag gcaggggatg ctttctatga gaggagtatt
     1741 attcccgaag gagatcgcaa tgcccttaaa acaggaaacg caaaatgaga ataaagaaac
     1801 caggcagcac cttctggaat gtgccaaaag ggagtttctg gaaaaaggat atatgaaagc
     1861 ttccctgcgg aatatctgta aagaggccgg agtgaccaca ggggcgctgt attttttctt
     1921 caaggataag gaagatctgt tggcagcact ggtggaagag ccgttgcggc agctgtatgc
     1981 aataatggaa gaacattatg ccacagagat gcacaacgag gatcaggtgg cacagctgga
     2041 tattgcggat gacggggatc tggaagcatc cagacagatc atccggcaga tgtatgcgaa
     2101 ccgggaggcc tttctgctgc tcctgacgaa atctcagggg tcccgatttg aaaattgtct
     2161 ggatgaggtc gtggatatct cggagcagca gtaccgcaga ttatgcgata tggtgacgaa
     2221 tgccaccgga agaccgcggg tcgatgatta catgacacac tggatggccc acattatggt
     2281 agatactttt gtacacctgt ttctgcatga gacagaagag agagtggcat tgaagcatgt
     2341 ggatgccttg acgatgtatc tggtgcgcgg atggatgggc ataatgacag aaagctaaaa
     2401 tacaatgcac cccaaggaag tttggacttt gcaggggtgc tgtttcagag cattaacata
     2461 acagtgttat gttaatgaaa atcattatca ttagtgatga ggaggcagga tatgtatctg
     2521 gaagtgaagg atctgaagaa gagctatgga gaaggcggcg gttatgtaca ggtattaaag
     2581 ggaatcaccg caggagtgga acaaggtgaa atgtgtgtca tccagggcac cagcggttcc
     2641 ggcaaatcca cgctgctcaa ctgcatcgga ggactggata ccgtggattc cggttccatc
     2701 gtagtggatg gacaggaggt ggcggggctt aacagtaaag cactttccaa ttaccgtcgg
     2761 gagaagctgg gttttatttt ccagttttat aatctggtgc ctaatctgac ggtgaaggaa
     2821 aatatccagg tctgccagta tctgacgaag aatcctttga atatggagga actgctgcag
     2881 gtactgggac tgacggaaca tcagaacaag ttccctgcac aactttccgg cggacaacag
     2941 caacgctgtg ccatcgccag agccttgatc aagaatccga gccttctgtt atgcgatgag
     3001 cctacgggtg cgctggattc caggacttcc cgggacatcc tgatactgct ggaggatatc
     3061 aacagacagt atggcactac gatgcttatt gttacgcata ataattccat taaaaacatg
     3121 gtacatcagg tcattatgat caaggacgga cagatcagca agaactatcg gaacgagaaa
     3181 cgtgttcctg cggcagaact ggaggatttg taggatgcag aggatattaa gcaaacgagt
     3241 acttcgggat atccgggaaa acctgcttcg gtatctggca ctgtttttcc tggtggcact
     3301 ggtcatgtat atggtggtgg ctattgtggg agctgccgag actattatgc aggggacgaa
     3361 ggagagcgga cgtgtccatc acagagagga cggacagttt ggcgtttttg ttccgctgac
     3421 cgaaaaagag accgcacaga tcacggaaaa aggtgtgaca ctgcagatgg atttttccct
     3481 ggattttcat ctgggacagt ccacgtttcg tgtctatcag gcaagagaat cggtagatct
     3541 gttcgttcca gcagaagggg aggaaattcc ggcacaggga gagatcctgt tggaacagca
     3601 ttatgcggaa aaacatgagc tgtaattagg agatatcttt atcgttgggg gaaaagagtt
     3661 caccgttgcc ggaatcggca gcacgccgga ttacgatgct gcttttgaga aaacgtcaga
     3721 taccactgta gacagcaatc tgtttggctt cggatttgtc acggcagagg attatgaagc
     3781 tctgaaagca ggcggacaga atttctggac ggaggattat acttacacct atctgctgaa
     3841 tgataccatg acggatcagg aactgaagga actgttacaa tccttcgaac tggaccgcag
     3901 taaggtgacg gatacttatt ttctggaaat gctggcagat gcggaagaga cgaagaacga
     3961 tattcaggat ggtatccagg aacttctgga tggcgtggat gagctggtgg acggtgtgga
     4021 tgaactggcg gatcataata cagaactgac ggatgcggcg gatgagttgt ttgatgccat
     4081 gctggaacag gtgaatgaca gcctgaagga tgccggagtg gaagtgacac tgacttcagg
     4141 aaattatgaa caacagctga atgccatgat cgctaatcct catgcctaca ccgcatccct
     4201 gtggcagaat ctgcaggacg cgaaaaagtc tctggaagat ctgcaggagt tccgggatgg
     4261 tgtgaaatct tacacggacg gagttaatgc agcttctgcc ggcggcggag cactggtggg
     4321 cggtatgagt cagatcacgg aaaacagtgc tgcgttgaat cagggagcag atgcaatttt
     4381 taatgccatt ttgggcatgg tgaatgaaca gctcctggca gagttggaac cttatgcggc
     4441 atacgggatc agctttccgg gactgacggc tggcggttac ggagagcagc tggatcagct
     4501 ggcaactgtc tttacacaga tgggggcatc ccaggcagcg gggcagctgt ctgcagtgaa
     4561 aagtcagttg gacaccgtgg cacagtttcg ggatggggtg aaagcctata ccgccggtgt
     4621 cggagaagca gccggaggat cccaggagct gttccagggt ctgagcgtgc tttataccgc
     4681 cagtgagcct ctggtagacg gcacagatgc cgtggtggat gcactgatgg atatggtgga
     4741 ggcacagctg gaagaaagtg atattcaggt agaactgact gccgacaatt ataaggaaga
     4801 gctggaccag ctgatggcag agggcagcag tgtggatgcc aggctgcggg attccttgaa
     4861 ggacgcgaag gatactttgg cggatctgga ggatttccgg gaaggaatca tcgattatac
     4921 ggatgccgtg gatgagattg cggatggcag cagggaactg cgggacggtg tgcaggaact
     4981 gcaggatgaa gccaatgata tgatcgatga gtattttact ttcgatatcg acaatctgac
     5041 acagtttttg gtggcagggg ataatcccag aatcgatgcc gccagcgggg atgtgatcat
     5101 caataaatat gccggtatgg tatccggtat tatcctgatg gtcatgttca cttacgtgat
     5161 ctccgtattt gtggtacata atattgaaaa agagagcagc gtcatcggtg cgctgtacgc
     5221 tttgggtgtg acgcgagggc agcttttgtt ccattatctg ctcaatccca tgatgatctc
     5281 cttcttagga ggtgcggtcg gctgtgtcct gggcttttcg aagtatggta ccggctggca
     5341 gatgcaggac agtatcgtgt atttttccct gccacccatg gaaatcgtga ctccggccta
     5401 tctgctggtc tacagtctgg tcatgccgcc ggtgacggcc gcattggtaa actgtctggt
     5461 gatcagcaag aagttgaagc gcacggcact gtctctgctt cgaaatgaac agaccgcagg
     5521 cagggccggg aagatacaag atgtaaatct gggaaatatg aagttccttc acagattcca
     5581 ggtccgtcag ctccttcggg agatgcgttc tgccgtggcg gtggtgatcg gtatgtttat
     5641 ctgcctgctg gtacttttga tctctgtaga ctgctatgtt ctgtgtaaga actttggaga
     5701 cgcctgcctg gatgagacga cctatgctta tatgtatact tacaaatatc ccacggagga
     5761 tgtgccggag gatggtactc cggcttatgt ggagagtctg aaaaaagagg cttacggtta
     5821 taatctggat gtgacggtac tgggaatcga taaggataat ccatattttc cggtggagac
     5881 cagcaataag aaaaatgaga tcgtgatctc cagcgccgca gcacagaaat ttggcgtgaa
     5941 ggtcggtgac aaactggtgc tttccgatga agtgaacgag agagattatg cgtttactgt
     6001 caaagatatc gttcattttg cctccggtgt ttatgtattt ttagaccggg atgccatgca
     6061 ggaactgttc gatcaggaag acgactatta taatgtagta ttcgccgatc atgcactgga
     6121 catcgacaac ggcagactgt attctacggt atccagagaa aacgtggagg aaagctctca
     6181 gatatttacg gatatgatgg gacctatggt cagcatgctg gcggccatct ccgcactgat
     6241 cttcatgatc gtcatgtatc tgatgatgaa ggtcatgatc gaccgttctg cattctccat
     6301 ttccctgatg aaagtactcg gctaccgaaa gggagagatc cgcagactgt atctggacgg
     6361 gaacttctat attataatgt tgggagcatt gttgggagta ccgctggcaa aatggtccat
     6421 ggatctgatc ttcccgtatt ttatttccaa tgtggccatc ggaatggatt tacagttccc
     6481 gccgcagttg tacggcatga tctacggagg catcctgatc tgctatctgg tgatcaactt
     6541 cctgctggtg ggcagactga acaagctggt gcctgcggaa gtattgaaga atagagagta
     6601 aataagtaag taaatatagg taacagttca gccatgaaca tatctgggca cagaaatcat
     6661 gcttgcatga ttttgcgggc aaatatgttc atggctgaac tgttacctaa cttttttata
     6721 taaatacata ctaaccctat tgacaaacta ggaaatataa ggtaatatca tctaaaacat
     6781 agaaaacata tagatttact aggatataaa agacgaggag gatgacaccg tggcagcaac
     6841 ctttgatacg acagaacaga gaatccttaa gatcatagat gacaaccggg agaccatcgt
     6901 agagttcgca agagatattt acgatcatgc agagctgggc tacaaggaat tccgcacttc
     6961 gaaaaagttc gtggatttta tgaagagctt acagctgccg gtacagacag atctggccat
     7021 caccggagca aaagcctatt taaataaaga aaaggccgga aatgcatccc tggcattgat
     7081 cggagaactg gatgccctgg ggattccgca gcatgcacat gcaaatcagg agacacaggc
     7141 agctcactgc tgtggacatc acgcacagct ggcaggagtc atcggagcgg cgctggcact
     7201 tacggatgca gaggtggcac agaagctgga tggacaggtg atcttcttcg ccacaccggc
     7261 ggaagagtac ggtgagatcg aattcaagaa tcaactgatc gctgatggca agatcgctta
     7321 cggtggtgga aaatgtgaac tgatccgcat cggtgctttc gatgatgtgg atgtggcact
     7381 ggcgcatcat atttccttag agggaatccg gctgggaagc aacagtggca atggttttgt
     7441 atccaaggtg atccgtatca aaggaaaagc agcccatgcg gcgggatgtc cggaaaaagg
     7501 tgtcaacgcc ttatcggcag caagcctggg gttacaggca ctggcactga accgtgagac
     7561 cttccgggat gaggattgcg tgagggttca tccgatcctg accaagggcg gagatctggt
     7621 caatgtagtt cccaacgaag cggtgctgga gacactggta agaggcaaga ccctggaggc
     7681 tttcgcagat gcatccgtca agacagatcg ttccttcaag gcaggagcac ttgccatggg
     7741 agcagggtat cgtatcgaga ctatgccggg ctatcttcct tctctctggc agccggcacc
     7801 ggaagagatg gccgatgcgg tgaaagcact ggcaggagaa aaaacggtat acgaggtcag
     7861 accggaggaa cacagcggag gatccacaga tgtgggagat gtacagcatc tgcttcctgt
     7921 gatcactttc aataccggcg gtgctgtagg tggtctgcac caggtggatt tcgatatcgt
     7981 ggatgaggaa gaagcctatg tgctgacagc caagatattt gccctgagtg cttaccgaat
     8041 gttgaaggac ggcgcggaag tgacgaagcg gaccaaacag gaatatcatc cgagattcgc
     8101 tgataaacag gaatacatag cttttatgga cagctttaat aaggtggagg aagcggacat
     8161 tgagtaaagc agattataaa atagaaggaa cggttcccag agaactgttg gtaagtgaag
     8221 tgagaaaggc cgcaagacag ttcgccatgc agttcttcca tttcagtaaa gtattatatg
     8281 accagttcgg actggagaag accaaagata tcgtgagaca gaccgtattc gaactggcag
     8341 tggaccggtc ggatcagctg cgggaaaagg cactggcgca aggcctgaag gcagattccg
     8401 tagaagactt tatgagtgtt atcgaccttc ccttcaccgg atggatcccg gaatggggag
     8461 aagatcactg tccctatgcg gaagtgtgga gaacttattt tgacaaatat ccctggttcc
     8521 gagagatcgc accgttttac tgtgacgtga tcgacactac taccattgag aacttttcca
     8581 aatgtctctc ccacaggatc acacagaatg tcatcctgga aggtacctgt tgtaagagag
     8641 agtactttga aagcgataag gtgaagcgag gagaatacac ttatggaaaa aaagaagaaa
     8701 actaagaata agaaacgttt tacctgggtg aatctggcac agctgctcac cgtggcggca
     8761 ctgcttctgt ggatgcaatg ggcagtagat tccggcaggg tgcttacgat cttcgtggcg
     8821 tccccgactt ccatcgtgga agagggcatt aagatcatta cagacggaac cttatggccc
     8881 cacttattgc tgacgatcca ggaggcactg gcaggttacc tgtccgcagt ggtagtcggt
     8941 attgcggtag gtctgttgtg gacattgttt ccggtatcgg agaagtacat gaatgtattc
     9001 tgttcggcaa tcatggcagt tccgaaagta gcaatcctgc cgctgttgat cctgtggttc
     9061 ggcatcggct tccagtccaa ggcatttctg gtatttctgt tctcggtatt tacgatcctg
     9121 tacaataccg tgaccggagc caaggagtgt aaaaaggaat acctgaaggt ggcaagggtc
     9181 tttcgggcca aacgtttcca gaccgtattt ctggtcatca ttccggcggc actcccttcc
     9241 atctttaatg gattgaagct ggcagcggca acggcactga ccggtgtatt gttctcggaa
     9301 atgcagtcgg caagagcagg acttggctat ctgctgacag agtcccagaa cctgttgaac
     9361 accccgagga tgttcttcct gatcatcctg attaccttat tatccgtagg tgcggtaaaa
     9421 ctgatcgatg ccatcgaata tgctatcagc tatcgatgga gacatgtata agcaaataaa
     9481 ttttttcaat agacgaggag gaaaatgtta tgaaaaagaa agtattcagc gtattactga
     9541 ctgtggcact ggcaaccgga gtactggcag gctgcggcag cagtgcagca accggcaaca
     9601 atgtagcaac cggagccgca cagaatgagg gaagccagac agcaagtgca actaccgcag
     9661 gagaggaatc cggagacacc atcgtgatcc gttcctgctt cgctaccgga atgaccggtg
     9721 cggtgaaccg ttttgccatt gaaaagggtc tgtataaaga gcttggtatt gagttcgaaa
     9781 acgtagaggc cagcgccgat accaactcca cagtaatgtc cctgatcacc agaggagaga
     9841 tcgatgtggc agacggagat cccagttctt atatccccgg tatctacaac ggtgtgcctg
     9901 ccaagctggt aggaaatatg tggagatatt ccggatgcta ctggctggtg gcaaacaatg
     9961 atatacagga cttctccgat ctggaaggca aaaaagtcgg taccgcagga gcttccggtg
    10021 gaatgagact ttccgtattg aagatgttgg agaagaacgg tgtcagcacg gataacgtgg
    10081 atctgatcgc caacggcgta taccagaccg cttatgcatc cctgacttcc ggcgaagtgg
    10141 acgctacgat cattcacaac ccgtatgcgg cactggctga ggcagacggc accggacata
    10201 tcctgggaag agcctgggac tacatccccg actactatac cggaacgatc atcgcaagca
    10261 acagctttat tgaaaatgag ccggagaagc tgcagcgctt cctgaccgct tattatcagg
    10321 tacatgaaga agttaagaat gactatttcg acgaatttgt tgcatgggca gcaaaagaga
    10381 tgaatacatc cgaagaggtt atgagaaagg ccatcgagtc ggaaatcgat gtatggctgg
    10441 attatccggt aatccccgaa gatcgtctgg ctaccacctt tgaatatctg aaggagtacg
    10501 gctgggtaga tgaaaatgtc agctacgaag gaacctacac caacgaattt gcgcaggtag
    10561 ctgcagatac cctgggaatg accgacccgg aagcaaaata attgagaatg ctctgaaaat
    10621 aagagacaca ggaaacggag gtaatggaaa tgcccgcaat atccattaaa aatatcaaca
    10681 aaacctatat cgatcaggat ggaaacgaag tacaggcact gcatgacatc aatcttgatg
    10741 tggcagaagg tgaattcgtc tgcatcgtag gtcccagcgg atgcggtaag agtaccctgt
    10801 tagagatcgt ggcgggcctg ctggatgcta caagcggcga agtattgctg gatggacagc
    10861 cggtacacgg taccagcaga gatattggcg tggtattcca ggatgcctcc ctgtttccct
    10921 ggagaaccgt acggaaaaat gtggcattcg gactggagat cgccggagta cccagggaag
    10981 agagagaaga acggttaaag cgttatatct ccatggtcag cctgaacggt tttgagaaca
    11041 aatatccggc acagctgtcc ggcggtatgc ggcagagagc cggcatcgcc agaacccttg
    11101 ccatgaatcc caaggtcatc ctcatggatg agcccttcag tgccgtggat catctgacca
    11161 aatgcacact gcaggaggaa ctgatcaata tccgtcaggc agagaacaag acggtattgt
    11221 tcgtcaccca tgatatcaac gaagcggtat ttctggcaga cagagtcgtg ctgttaagcc
    11281 caagacccag tacgatccag aagatctacg agattccgct ggagcagaag agaggacgca
    11341 gtgatcctct gctgttaaag atcgcagcgg aaattgccaa agatattaac agtggcggac
    11401 aggatattcc caacaactat tggggcaact tataggcgga gaatacagag aataaggagg
    11461 cagcagtatg tttcgtgaca ccagtatgga ttacctttcc agagatgaag tacttgagat
    11521 ctggaataac aatatgcgtc tgacagacga gcagctttta gccatggatg agacagaatt
    11581 ccgggcaaga gtgagagaac gttcccatca taccctggag atccaggtat atgccgccgc
    11641 atatcgccac cagaaattaa aacccaacca ggcagattat acgaagcatc tgctggaact
    11701 gtgggagaaa agaggattgg gaaaagatct gccggaatat cggtatgcct ctttcctgat
    11761 cgatgcggcg gagaaactgg taaaggggga agacgtggat ctgacacctt acaaacctac
    11821 gccggtgacg gaacagatgg agaaagactt tttcacgatt gtaaaggaac gcagaagcgt
    11881 ccgggaattc aaggacaagg aagtaccgga tgaactgatc gataagatcc tggaagcggg
    11941 gctttgggct gctcacggct gtaatgtaca gtccatccgc tatgtggtgg tacgggagaa
    12001 aaacgagccg ggactgttcc gcggcagtga cgtgccggga ggtccggtgc atctggtgat
    12061 cttacaggat atgagatgct acagagccaa ctcctttacc ccagtgagaa accagctgct
    12121 ggatgccgga gccgccggac agaatatcgt tctggcagcc cacgccgtag gactggaagg
    12181 tgtctggctg actttccccg gagaagaatt ttgtgacaga ctgcggaaac gcttcaatct
    12241 gccggattat atccgcctgg tgacctatgt ggatgtagga tacggtgacc agactcctta
    12301 ccctcctcag agatgggacg taaaggatac gattctcgga agatactagg aagaaggaga
    12361 gcggcgcatg aatattgtga tcatcggtgg cagccgcggc atcggcaagg cggccgccag
    12421 agaattgtta caggatggaa ataacgtgct gatcgttggc agaaatccga agacactgga
    12481 agagacaggg aaagaactgg ccggagcggc aacgtattcc tgtgatgccg ggaaggcaga
    12541 ggaaaccact ggactggtgg atgcagtgaa gaacggtaaa atagcgggat tcggagataa
    12601 cgtggatgga ctgatcctgt gtgcggcggt atttcccaat ccggccacga agacatccgt
    12661 agtacagccg gagccggagg aactgcaggc aatgcttgac agcaacgtga cggcatatta
    12721 ccgtctggtg aaggaatttc ttccgctgct ggcaccgggc agccgcatcg tactcatcgg
    12781 ttccacttcg ggaatccgca gagacagggg cggcatctac ggcatttcca aatgggcgct
    12841 ccgttcctat gcctatgccc tcagggagga atgcaagcct atggggatcg gcgtatccct
    12901 catcaatccc ggcggcacct ttaccgagac cagaaagaaa tcctccgaag aggacaggtc
    12961 acttttagag acctctgacc tcggaaaagt catcgccctg accttccgtc tgtccccaca
    13021 ggcagtactg gaggaagtga atatccgacc tttacaggga gatacctatt aggcagtcga
    13081 tggactgcct tttttcttcc caaggaaact cattgaatgt taaaaaaaag tatggtatga
    13141 taaagggcag aactgacaaa gatacgagga tcacggaggt ttttagatac aatggaacag
    13201 tacaagcagg aatttattga attcatggtg gattgtcagg cgttgaagtt tggcgagttt
    13261 accttaaaga gtggcagaaa gtctcctttc ttcatgaacg caggtgcgta tgtcaccggt
    13321 tctcagctga tgaagctggg cgagagctat gcgaaagcaa ttcacgatac ctacggtgac
    13381 gattttgatg tattgttcgg acctgcctat aagggcattc ccatcagtgt ggtaaccgca
    13441 gtggcttaca gcaaattata cggcaaagag gttcgttact gttctgaccg taaggaagag
    13501 aaggatcatg gcgcagataa gggaagcttc ctgggaagct ccttaaaaga tggcgacaga
    13561 gtcatcatga tcgaggatgt taccacttcc ggcaaatcta tggaagagac cgttcccaag
    13621 gtaagaggag cagccaatgt agaaatcgtt ggtctgatgg tatccctgaa ccgtatggaa
    13681 aagggtctgg gcggcaaggt cagcgcactg gaagagatca aggaaaagta cggatttgat
    13741 gcacatgcca tcgtaaccat gcaggatgtc atcgatcatc tgtacaacaa gccttacaag
    13801 ggacaggtat acattgatga tactctgaag aaggcaatcg acgaatatta tgctgtatac
    13861 ggtgcttaag cagacggctt cgaaagtttt ggatagaaga caggaggatt ccatggaaga
    13921 gaaaatctat aagatcatgc gtggagcagg tgctctgaat attgtactgg gagtcatttc
    13981 cctggtagtc ggaatcgcgg caggtgtgtt gctgatcatc agtggcggca agctggtctc
    14041 cggcaaatca cgtattctgt tctgatcaca aatgagaagg aaaacgccgt cagggcgtga
    14101 aaattgggca tttccatacc ggaagtgccc ttttcgggtt ccatggggga gtgcaggtta
    14161 ctgaaaggct tgaaaatagt atgcggaaaa agaactacat ttttacgaat aaaaaacatt
    14221 ccaacagggc gatcatgtcg accatcctgg ggggaatcag tctggtgtcc ctggggatcg
    14281 tgacgtatct gtcctacagt cagggcggcg tgatgcacgg tggatacggt gttaccggtg
    14341 tgttggccac catctattcc ctgatcggac tgatcctggg aatactgacc cttcgggaaa
    14401 aagatatcta tcgtatcttt cccgtgctgg gaacaatatt aaatgttgtt gcccttggag
    14461 gtgtcagctt tttgctgtat ctgggaagcc gtctgtaacg gatattctgg agcagaagca
    14521 ttggatcaca cagcagttat ttagatagaa ggagcaatca gatctatgga agaaagagaa
    14581 ttattattgg aacgtctgga actggcctta gaccggatcc gggagatccc cggggaggac
    14641 tggcaggaag aaagcctgca gcactggaaa gaatatttta cagcggtagc aaaattttta
    14701 ctgctgatcg aggataccag acagttcctg gagcagggaa aacagaacac tgccactttg
    14761 gaagagctga aacagcgtaa ccatgccctg tatgaagata ttttaccgga aaattatgag
    14821 aacagctttg ctaatcctgc ggtagctgta aagtgtctgg gagaagaatt cggcgcactg
    14881 gccgccttcc tttatacgga aatgcggagc ctgatcggtt ttacctatga gggaagactg
    14941 gacgaactgg tgatccgtat ggaactgttt tctgaggtat atgcggcatt tgtatatgag
    15001 gagcaggaga atcacagact tccgtcttat gcgtccatcc gtgagatcct ttactggttc
    15061 gtcagcgatt acgcggatgt ggcggcggaa gcacgggtac gggaactggt atgcccgggg
    15121 aataattttg cggcagacat tatccgaagg gcggaactga ccgatctgcg ttatctctat
    15181 gcctatggag aatacgtggg ggaaaatgaa ctggcaatgg cgaaattcct gaattccctg
    15241 ccggaagaga ccataaatac catggcagac acttataccg aggggtaccg catcggtttt
    15301 gaagtgaccg gcaaggatct gtcgaaaaaa gctgcggtgg atgtccgtta tcagttgggc
    15361 ttcgaacgca tgatgcggag agctctggag aattttgaca aaatgggatt gcagcctgtg
    15421 atctaccgtg cagctgccag tattttatat aatccctcca tttataaaaa cggcttctac
    15481 agtgtcagcc ctaacagaca gtatgagttt gatcacaagg atgacaaagc actgttcttg
    15541 gataaaatgt attgcagccg gaaactggaa gtcatgcata ccgcttttga aaaatataag
    15601 acagaggcca gaagctatgc gggcccggcc gtggtggaga ccttcggtga gaaagatttc
    15661 gcaccggtga acaagccgga agcggtgaaa atgacggatg aacagagcac tcttatggtg
    15721 gagcaccgca cccagatggg acagttgcag agacagtata ttattgagga agagcgtagc
    15781 tttacgatta ttgcgttccc catcccggag gtaggggact gctttgaaga acttttccgg
    15841 gagactattc ggatcaacac cctggattat aaaaaatatc agaggatcca gcagaccatc
    15901 atcgatgcgc tggatcaggc agaccactgt gaggtgaagg gctgtaacgg taatcacacg
    15961 gatatcacag tgaatctgtg gaagctcaaa gatcctgcca aagaaactat ttttgaaaac
    16021 tgcgtggcag acgtgaatat tcccgtggga gaagtattta cctctccggt gctggaaggc
    16081 acaaacggtg tgctgcatgt gacaaaagtg ttcctgaacg gcttggaata taagaatctg
    16141 gagatcacct tcgaaaacgg caggattaaa aactacaact gtgctaattt tgccacggag
    16201 gaagagaaca aggcattcat taaggaaaat atcctgttcc gccataagac actgccgctg
    16261 ggagaatttg ccatcggtac caacaccacg gcttatgtgg cggcaaagcg tctgggcgtg
    16321 gaagccagga tgccgatcct gatcgcggag aagatgggac cgcattttgc agtgggcgat
    16381 acctgctatt cccatgcgga agaggtgaag gtatacaatc ccgacggtaa agagatcgtg
    16441 gccagagaca atgaggtggc cgcccttcgc agtgtgaacc cttccaaggc ttattttaac
    16501 tgccatacgg acattaccat tccttacgat gaactggcag agctgaccgc agtgaaaaag
    16561 gacggcgggc ggatacccat tattgcgaac ggcagatttg tccttcccgg aacggaagaa
    16621 ctgaatgagc cgctgcggga gttggattaa aactattcag caagcatttg cagaaagcaa
    16681 ttttgcatgg ctctgaatag ttatcataag atttcataaa accattgcat cattgaaaag
    16741 attttagtaa gataaaatat atatggataa aatcggtgca accgatacat gaggaggaca
    16801 ttatggcaaa tacagagaat tgccctaagg ttggcattgt aatgggtagt gattccgata
    16861 tgcctgtgat ggcgaaagcc gcagacattc tggaagaact gggcatccct tatgagatga
    16921 ccgtgatcag cgctcacaga gagccggatg tgttttttga gtatgcaaaa agtgcggagg
    16981 aaaaaggctt caaggtgatc attgccggag ccggtatggc agcgcatctg cccggcatgt
    17041 gtgccgctat tttccctatg cccgtcatcg gaatcccgat gcacaccact tccctgggag
    17101 gcagagattc tctgtattcc atcgttcaga tgccctccgg catcccggtt gccacagtag
    17161 ccattaacgg cggcgccaat gcaggacttc tggcagccaa gatcctggct acttccgacg
    17221 cagaacttct cggaaagtta aaagcataca gcaccaaatt aaaagaacag gtagaagcca
    17281 aggatgcaaa attgcaggaa atcggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnngcaga gggctgtaag cagtctgacg cagcactggt aggcggtgag accgcagagc
    17461 atcccggact gatgcctgag gatgagtacg atctggccgg atttgcagta ggtgtggtag
    17521 agcgtaagga tctgatcacc ggcgagaaca tcaagcccgg tgatgtactg gtgggaatcg
    17581 cttcctccgg tgtacattcc aacggtttct ccttggtgag aaaggtattc gatatgacca
    17641 aagagagcct ggatacctat tatgacgaac tgggcaagac tctgggtgaa gcactgcttg
    17701 ctcccaccag aatctatgtg aaggctatga agtctgtgaa gaacgcaggc gttaaggtaa
    17761 agggctgcag ccatattacc ggcggcggtt tctatgagaa tattcccaga atgcttcctg
    17821 acggcatctg cgcagttgtg aagaaggaca gctacgaagt acccgctatt ttccgtctga
    17881 tgcagaaaaa gggcaacatt gctgacgaga tgatgtacaa cacctacaac atgggtcttg
    17941 gcatggtgct ggcactggat cccgcagatg tggacaagac tctggaagca ctgaaggcag
    18001 caggggagac cgcttacgtg gtaggtacct gcgaagccgg tgaaaagggt gtgaccatat
    18061 gttaagagta gtcgtactgg tatccggcgg cggaacgaat ctgcaggcga tcattgatgc
    18121 tatcgcagcg ggcaaggtca ccaatgcgca gatcgtcggt gtcatcagca acaacaagac
    18181 ggtgaagagc ctggagagag cacgtaatgc aggaattcct gcggtatgtg tgtctccgaa
    18241 ggattatgag agcagagaag tatttaacga tgcgttgctt gcaaaggtga aggaatttac
    18301 cccggatctg atcgtgctgg caggctttct ggtgaagatc ccggaggcta tggtgcatga
    18361 atacagtaat cgtatcatca atattcatcc gtctctgatc ccctctttct gtggtgtggg
    18421 atactatgga cttcatgtgc atgaaaaggc actggagcgc ggtgtgaagg tcaccggagc
    18481 caccgtgcat tttgtgaatg aaggcatgga tgaaggccct atcattgcac agaaagcagt
    18541 ggcagtggaa gatgatgata ccccggaaag cttacagaga cgtgtcatgg aacaggctga
    18601 gtggctgctt ctgcctcagg cggtggatga cattgccaac ggacggatct gcgtcgtaga
    18661 cggcaaagta aagcataaca aatgagtaac tattcagagc caatgggcga agtaatcacc
    18721 atgagcaaat agcatacaaa catgttattc aggaaagcct cgcagaggcg gttttgcgaa
    18781 tggggggtga atagttacgg aataaggaga aacataatat gggtatgaaa gtattgatcg
    18841 taggtggcgg tggcagagag catgccattg caacctgtgt tgcaaagagt cctaaggtag
    18901 ataagatcta ttgtgctccg ggcaatgccg gaatcggtca gatcgcagaa tgtgttccca
    18961 tcggagccat ggaatttgac aagatcgttg cttttgcgaa ggaacaggcc gttgacctga
    19021 ccatcgtagg tatggacgat cctctggtag gcggactggt agatgcactg gaggcagagg
    19081 gcctgcgtgc tttcggaccc cgtaaaaatg cagcaatcct ggagggcagc aaggcttttt
    19141 ccaaggatct tatgaaaaaa tataatattc ctaccgcagc ctatgagaat ttcgataatg
    19201 cggaggatgc catcaaatat ctgaaggagc aggcaagttt ccctatcgtg ctgaaggcag
    19261 acggcctggc actgggaaaa ggcgtactga tctgcaatac gttggaggaa gctctggcag
    19321 gcgtggatga gatcatgctg gacaagaaat tcggtgcggc tggtaatcgt atggtcatcg
    19381 aagagtttat gaccggaaga gaagtcagtg tactttcctt tgtagatggc aagaccatcc
    19441 ggatcatgac ttctgcacag gatcacaaga gagcgaagga cggcgatcag ggactgaata
    19501 ccggcggtat gggaaccttt tctcccagcc cgttctatac cgaagaagtg gatgcattct
    19561 gcaagaaata tatttaccag gctacggtgg atgccatggc agcggaaggc agacctttca
    19621 aaggcgttat tttcttcgga ctgatgctga caccgaacgg ccccaaagta ctggagtaca
    19681 atgcaagatt cggtgacccg gaggcacagg tggtactgcc ccgcatgaag aacgatatta
    19741 tcgaggtcat ggaggcttgt gtggatggta ctctggatca gatcgatctg cagtttgaag
    19801 acaatgctgc agtctgtgtg gttctggcca gtgatggcta tccggtatcc tatgagaagg
    19861 gttatgtgat aaacggactg gagaagttca acggaaaaga cggctattat tgtttccatg
    19921 cgggaacgaa gctcacagac aagggaatcg tgacaaacgg cggacgtgtt ctgggagtga
    19981 cagccaaggg agctaccctg aaggaggccc gtgccaatgc ctatgcggca acggaatgga
    20041 tcaccttcga gaacaaatac atgcgtcatg acattggcaa ggcaatcgat gaagcgtaag
    20101 acatgtcagg atacagaagg agttgcagat gaaaaagaca gtacagagaa caagtaaatt
    20161 caaggctttc tggggaatgc ttgcattggt gcttgtgctg ggagcaggac tttttgccga
    20221 gaatctggcg tttgtcagcc atgcggaaag cgcagccaag gtaacagcat ccagtgcaaa
    20281 gatccgtaag agtgcggatt cttccagtga agtcatcggc agtgcggcaa aggataagac
    20341 catttccatc aagagccaga ccaagggagc ggatggctat acctggtatg aggtatatgt
    20401 ggatgcgaat acgctgggat atattcgttc cgatctggta agcattaccg acggatctac
    20461 cccgccgtct tcttcaacta cgaccaccac gacaactaca acgacgacta ccgcagcaac
    20521 accggctccg gcagtgaatg aaacaccggt ggatgtcaca gcggtggagc ctgtaagtgc
    20581 taccgtgacg ggcggacagt cggtgcgtgt gcgcagcaat gcatctacca cgagccagat
    20641 cgtaaccaca gcagagaacg gaatggctct gaccgtcacc ggacaggcta ccggtacgga
    20701 cggcaatacc tggtatcagg tgaatttcat cgccaacaat accgaagtga ccggatttat
    20761 ccgttccgat tatgtggcgt tatccggaga actgcaggca ccttccacag agcagcctgc
    20821 ggaagaacag ccttcggaag aacagcccgc cgaagataca cagaccactt ccaaggactg
    20881 ggatacccag ttgcagggag atgcatggta tctgttagat atggtaggac agaaacaata
    20941 caagatcgat gatctgttca gctctattga ccagattact gagatcaatg cgcagtttga
    21001 gaccaatcag aagaagatca ccagtcagaa ggtcgtgatc attattcttg tcattcttct
    21061 ggtggcagcg gcggcagcag tcactctgct gatcttcaag atcaaggata tgacggatgc
    21121 ggctgatttc tctgatgcgg aggaagcagc acttcgcaga agaagagtgg acagacccca
    21181 gaccggcaga ggtcagggtg gaagaccggc acctcagggc ggcagaaccc agggaggcca
    21241 gaaagtgatg catgaagtgg gagcagagag acgtcccgca ggaaagcccg ccggacaggg
    21301 cggcagaccg gtgcagggac agagaccggc aggccagcag gcaagacccg cgacacaggg
    21361 agccagacct acggcacccc aggccggaag acccgcaccg cagggaacca gacctgcagc
    21421 acc
//
LOCUS       DAWEJW010000046        21385 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_974_length_21385_cov_54.128926, whole genome shotgun sequence.
ACCESSION   DAWEJW010000046 DAWEJW010000000
VERSION     DAWEJW010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 21385)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21385)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21385
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_974_length_21385_cov_54.128926"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5007..5106
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6841..6940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11400..11499
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14683..14782
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16189..16288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5816 a         4376 c         5537 g         5156 t
ORIGIN      
        1 cctgcggaaa tgaggagaaa atgacaaaga tcagaacaag atacgcaccc agccctaccg
       61 gtaaaatgca tgtaggtaat ctgcggtccg cattatatga atttttgatt gcaaagcatg
      121 ccggtggaga tttcatgctc cgtatcgaag ataccgacca ggagcgctat gtggaaggcg
      181 ctaccgagat catctatcgt accctggaga agaccggact ggtacatgac gaaggcccgg
      241 ataaggacgg cggttatggt ccttacgtgc agagtgaacg tatgaaaacc ggtatttata
      301 tgaaatatgc caaggaactg atcgataagg gagaagctta ttactgtttc tgtgacaagg
      361 agagactggc ttccctgaag accgaagttg tggatggtaa ggaaatcacc gtatatgaca
      421 agcactgcct gtccctgtcc aaagaagaga ttgaggcaaa tcttgccgca ggcaagcctt
      481 ttgtcattcg ccagaacaac cccaaggaag gcaccacgac tttccatgac gagttatacg
      541 gagacattac agtagataac tcccagctgg atgatatgat cctgatcaaa tccgacggtt
      601 accctaccta taactttgca aacgtggtag atgaccatac catgaatatc acccatgtgg
      661 tacgaggcaa cgaatatctg tcctcctccc cgaaatatgt acgtctgtac gaagctttcg
      721 gatggcaggt accggtatat atccatctgc cgctgatcac cgatgagaac cacaagaagc
      781 tgtccaagag aagcggacat gcttccttcg aggatctgat cgatcaggga ttcattaccg
      841 aagccatcgt gaactatgtg gcactgcttg gatggagccc ggaggataac cgcgagattt
      901 tctctctgga tgaattgatc aaggagttcg attaccatcg catcagcaag tccccggcag
      961 tcttcgatat cgtgaaattg cgttggatga atggtgaata tctgaaggct atggatttcg
     1021 acaagttcta tgagatggca gagccttacc tgaaggcagc gattagcaag cctctggatc
     1081 tgaagaagat cgcagccatg gtaaagaccc gaatcgaagt attcccggat attgccggac
     1141 tggtagattt cttcgaggag cttccggaat atgacgtcgc aatgtataca cacaagaaga
     1201 tgaagactac cgcagagact tctctggagg tattgaaaga tctgcttccg atcctggagg
     1261 cacaggagga ttacagcaat gacgggctgt atcagactct tgtaaaatat gtggaggaaa
     1321 aaggctgtaa gaacggctat gtgatgtggc cgatccgtac cgcagtatcc ggcaagcaga
     1381 tgacacctgc cggtgctacg gagattatgg aagtcctggg caaagaggaa tctctggcaa
     1441 gaatccgcaa aggaatcgag ttattacagg caaatgtctg atctgacatg cttaaatgcc
     1501 tcccagcagg aggctgtcac gttcggagag ggaccgctgt tattactggc gggccccggc
     1561 tccggcaaga catttaccat cacccagagg atcctgtatc tgatccggga acgccggatc
     1621 accccggaga agcttctggt gatcaccttt accagagaag cggctctatc catgaggcag
     1681 cgctttgcgc agatatctcc acaggaagtg cataccgtca attttggaac gtttcattct
     1741 gttttctatc aaatcctgct ccagtcgggc agaatttcat caggcaatat tttaaacgac
     1801 agacaaaaaa gagatcttat ccttcccatt ttgaagaaaa taacagactg taaaaaaaga
     1861 tccgcagcgg aagtaacgga gcttgccggg aactatcttg ctgccatcgg ttattacaaa
     1921 aataccggcg atattgagaa cagcagaaag aagcttccgg aagaagaaaa acaacatttt
     1981 tccaggatct atgaagctta tgaggaggcg agaaaaaata ccggaggcct tgattttgag
     2041 gatatattaa aggaatgtga ggagtttctg caaagggatc cggggcagcg tacatactgg
     2101 cagaaccggt ttgaatatat actgattgat gaatttcagg atatcaatta cagacagtac
     2161 agtatcgtga aaattctggc ggaaaaacat agaaatattt ttgcagtggg ggatgatgat
     2221 caggccattt acggattccg cggagccaga ccagcctgca tgagacagtt tgtagaggaa
     2281 tttggtgcaa agcagatcct tcttcggacg aattatcgca gtcatccgga tatcgtggag
     2341 gcatccctgg cggtgataga tgagaataaa gaccgttttc ataaggatct ggagccttgc
     2401 gaggcgcata gacggcaggc aggggatcat aagactgacg ccggataccg gaaggaatat
     2461 cgggtaaaaa tcagggaatt cagtgaggct tcggaacaaa tgagttatct ggtacaaagc
     2521 ttgaaaaatc ggataaatgg ggagacctgt gctgtattgt tccggacaaa tctggccatg
     2581 cagggcgtgg cggcgagact taccggggag gggatccctt tttccatgaa agagaagggg
     2641 agaaacattt acgaccattt tatcgcacag gatcttctgt cctatctgcg gatcgcgcag
     2701 gggtgtgcta caagagcgga ttacctgagg gtgagcaaca gaccatatcg gaatatttcc
     2761 agagaggctt ttggtgtcga agcatcttta ccggctttga agaaatatta tgaagatctg
     2821 actgcgggaa gtccggacgc ggggcagcaa aaggcgcaga aagcggtcgg agtatggata
     2881 cgacagatgg aatttgtaaa aaatacggaa ctgaagcttg caatggcatt tttgcgtaag
     2941 gcgtgcggct atgagagata cctgcgggtg agagctgcac aggagggaaa aacagatctg
     3001 acggaatggc aggaagtgct ggattttatc acggaggatg cgggacgata ccgaagcctt
     3061 gaagaatggc aggaagcaca ggagctttac cgggaacaac tgcaatgcag ggaaactgta
     3121 aggagtggca tgcagaagaa gcaggaaaaa caggaaaaca gcgggatcac tctgctgacg
     3181 gtacatgcgg caaagggact ggaattcgac catgtgtgga tcccggactg taatgagaag
     3241 acctttcccc acggaagctc ccgggagccg gagcattgtg aagaggaacg acggatcttc
     3301 tatgtggcga tgacacgggc aaaaaaagac ctggaacttc tctgccttac aggcacgaga
     3361 gagcgtccca ggttcccttc cagattcctt attcctctga atcgttatca tcgatgatct
     3421 cgtcaaattc acagctgtcg agatactcat caaaagcatc ggcaaccttt tcgtagacat
     3481 catcatcttc aatgtatgcg agaatgggtt cttcattcgg gtcatccgga ttctcggaat
     3541 atccgtagaa ccatacttct ccgtcttcgt tttcgccgtc ctcgtccaga ggcaacagta
     3601 cgatatagtc cttggcctct acggtgagaa tggtaatgat ggcacagttg acaacagtgc
     3661 cgtcttccag ctccagctca acggtcattt cttcatcttc atagctttct tttttaccca
     3721 tgggaatcct cctgatatgt ttattgggtg taacagtact ttgttccttt attataaccg
     3781 cttacggata ttcttgcaat gcttgttttt cttacactta cagactataa taaaaatgga
     3841 caaagagact gtaagtgata gaaaactgga agatggggta tgacaatgac agaaaaaaag
     3901 catgcaataa agccttaccc tctgggggcg catgtggagg acggagccat ccgttttgcc
     3961 tatgcgtccg ggaaaaagga ttgcggcatt ctcatatatg acagaaagag tggaaaaaaa
     4021 acgcacaggg ttccgtttcg acaggaggaa cggatgggaa atgtgtactg taaatatctg
     4081 gaactggagc cgggaaatgt cgggtatcag ttctatgaag gggagcgtac tgtgccggat
     4141 ctgcatgcac aggggttcct gcgtaagcct gcttatggga aaatacgtaa gcctgcggat
     4201 cgcattgcag tatttcccaa cgaagatttc gactgggaac aggatatgca ccctatgctt
     4261 ccctatgaag acagcacctg ctattgcctt catgtgcgtg gttttaccat gcatgcctca
     4321 tctcaggtaa cacatcgagg cacctttgca ggggttgtgg agaagctgga ctatcttcag
     4381 gaaatcggtg tgacaaccgt ggaattccaa ccggtctatg aattcgatga gacaccggag
     4441 aagacggtgc cgaagacctc cggagaactt gcggccgtgg cgggggaaaa gaacggagaa
     4501 ggtaaattgc ccggctaccg ggtgctgaac tattggggct atcgggaagg cttctattat
     4561 gcgcctaagg cagcctatgc ggcaggggaa gacccggcag aagagtttcg tcttatggta
     4621 aaagaattcc ataagcgaaa aatggaagtg atcctgcagt tctatttccc tcggggaatg
     4681 gatgcggcag agatcgggaa tattttaaga ttctgggtat tatcctatca tgtggatggt
     4741 tttcatctga tgggggaggg agtaccggct cagatgctgg ctggagatcc ggcattgtcc
     4801 gggacaaaac tgtggcatta ttcctttgat acggagcaac tctatgatcc ggcagtgaag
     4861 ccggaatacc ggaatctggc ggaataccgg gacgattatc tgtataccat gcgtcgtttt
     4921 cttaagggag atgacaatat gctatcgggc gtattgtatg agatgcgtca tatccccgcc
     4981 aatatgggac ggatccatta tctgtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnntcct gtggttatcc ggatctgtcc tatcacggac agaatgcctg gaggccccag
     5161 acagagagct ataaccggca tgtaggcatt atgtactgcg gaaagtatgc aaagacacca
     5221 cagggtgggg aggattcttt cctctatgtt gccatgaata tgcactggga gccgcagaaa
     5281 ctggcatttc caaagctgcc gaagggtatg gaatggaatc ttgtgctggc aacggagaag
     5341 gcggaagata ctctgacagt gcaggagaaa gaagaacagt ccagagagca gacggggatc
     5401 attgccccca gaagtattgc agtatatgaa ggcgttgtga tggcagcatc acaggacaag
     5461 gagaaaaaag tttcggcaag atctttaaaa aagaaaacag atgttgccgg agaaagatga
     5521 tagaacgaaa tgaatatctg gaaacatttt aaaacgatca cacatcataa gatccttgtg
     5581 gcaaagggct gctttaaggt aagtctgtat aaacagggat tgttgcatga tatgtccaag
     5641 tatagtccca cagagttctg ggtaggggca aaatactatc aggggacacg cagccccaac
     5701 aatgcggaac gagaagatat cggttattcc agcgcctggc tgcaccataa gggccggaat
     5761 aaacatcatt acgaatactg gatcgattac agcatgcagg cactgcccgg tggaatggct
     5821 ccggtgccga tgccggataa gtatattgcg gaaatgatca tggatcggat tgctgccagc
     5881 aaggtctata tgggaggcga atacacggac aggtctcctc ttgattacta ttatcagggg
     5941 acagataggg cacccattca tccggagacg aaggcaaaac tgctatatgt gctggaaatg
     6001 ctggcagagg agggagagga gaagaccttc cggtttatca aagaggaact ggtgcagcat
     6061 aaacggtgta tggactggca ccagctggag aaagcggaat agaatagtaa aaaacagtgg
     6121 taagtagagg atcctatgaa ctgtatcatt ctgttattgt tactattttg tggtggaaat
     6181 tcttgtagca acggctgtag ctgtatagga aacagcaatc gcagttcctg cggaaacaac
     6241 aattactgtg ggaattccaa taactcctgc ggaagcagta gttcctgtgg cagctcgaat
     6301 gcctgcggcg cacgttgtct ggaagacgag ggacgcggtg gagacagaga ccgcagaccg
     6361 cccagaccgg atttcccgaa tttcacaccg gtggatgatc cggcattttc caggagaaat
     6421 gacggaagat gcgagacctg tggatgtgag cagaattcct gaaaatatgg gtaacaggaa
     6481 agccccggaa ctgtcttggc agttcagggg cttgttttgt ctgaaagact agttggacat
     6541 aacttctttc cagtatttgt tgtagagttc ttcgccgtcc tctccgaggt acaggaaggt
     6601 ctccagattg ttatactgcg tcagatccgg gaaagcaatg ggagaattct tcagatcttc
     6661 atcctcgatc agctctctgg cggcggtatt aggagtaccg taggtgatgt actcgaaatt
     6721 cttcagtgct acatccggac gacacatgaa atcgatgaat ttctctgcat tttccttgtt
     6781 gggagcattc ttcgggatta cccagccatc gatccagaca ttggtgcctt cctcgggaat
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcccatttgg tgggaggggt
     6961 atctaccaga gtcttattat agatgattcc tacagttccc cagcagtagg gaacacagta
     7021 cttgttaccg ggatcgtaag cggcagcctg ctcataatac tcggcaccga tgttttcctt
     7081 ggcattgggg atcttgtcat aatccagttc ctggatcagg tcgttttcaa tcattttctg
     7141 taccatgtaa tcggaaggac ataccacatc ataagcggaa gaaccagctt ctaccttggg
     7201 atacatgatc tcgttggtct cgaactcatc gtagaccact ttgattccgg tctctttttc
     7261 gaacatggtg atcgtatcgg gatcgatata ttcaccccag ttgtagacga tgacttcacc
     7321 gttttcacta ccggcggaag aggacccgca gccggtcaga gcagttgcgg taaggatacc
     7381 gcacagaagc agtgcaataa attttttcat ttcttttcct cctgaatcta ttttcaaaga
     7441 attatttctt cttctttgcc atgggcatgt agttcaccag taaaagcaga accagtaccg
     7501 tcacaaaaat gatggtggac aatgcataca tttccggctt gatgcccttc tttacctcag
     7561 tgtagatttt ggtggacagt gtgtcgacac cgggaccttt ggtaaagtgg gtaatgataa
     7621 aatcatccag tgacatggta aatgccatca ggaaaccgga aaggataccc ggcaggatat
     7681 ccggaaatac caccttgaaa aaggcgtagg tctgggaagc acccaggtca agggcggcct
     7741 catacgtgct ggggttcaac tgcttcatac ggggcattac gcttaagatc acataaggaa
     7801 tattaaacgt aatgtgtgac agcagaatcg taccaaaacc aaatttggct ccaaggctta
     7861 agaacagaag catcagggag ataccggtaa caatctctgc gttcagcatg ggaatatttg
     7921 tgattcccat aaggatcgta cggctggtac gcttcatgga ttgcatggca atacaggcaa
     7981 tcgtaccaat gaaggtggca atcagtgcag acaacacagc cagcgccagt gtattccata
     8041 gagcctgcat gatctgttcg ttctggaaca gggcagcata ccatttggtc gtaaaacctc
     8101 cccattttgc acgggtcttg ctggcgttga aggagagtac gatcaaggta acgatggggg
     8161 catacaaaaa gataaagatc agccccagat atattttctg tgccgttgtt ttgatcataa
     8221 tgtgttcgcc tccccctctt tatccgtgat tacggagatc accatattga agataatgaa
     8281 cagcatcagt acgatggaaa gtccgcttcc cagattccag ttgccggtct gggtaaattc
     8341 ctgttcgatg acattaccaa tgagcaggat tttgctgccg cctaagatct tactgatcac
     8401 gaaagtggtc agggcgggga caaataccat agtgataccg ctgaacatgc cgggcagact
     8461 taaggggaag atgatccgta caaaggtctg cacaccgttg gcaccaagat cataagcggc
     8521 gttgattacg ctcttgtcga tcttgaccag tacattataa agaggcagta ccataaaagg
     8581 caaaaagttg tataccatac ccagtacgat ggcagagggt gtattaataa tatttaacgc
     8641 cggcagatgc agggtggaga ggatgctgtt gatgacacct gtcttctcaa gcagtgtctg
     8701 ccatgccagg gtacgaagca ggaagttcat ccacatgggc aggataaaga tcaataccag
     8761 aaacgagttc tggttgacat tcatgttaca caggatcatg gcaaggggat atgccagaag
     8821 caggcagatc acagtgctga tcacggataa cttcagcgct tcccacagag ccttggaatg
     8881 ctcggcggtt gcgatcgcca gaatattatc cagcgtaaaa gcgccgctct tatcggtcag
     8941 accatagtag aagaccatgc acaggggcac gatgacaaac atggctgacc agaagataaa
     9001 agggagcgat aataattttt tattcattga ctccaatcgc ctcctcatcc tcggattccg
     9061 gcttcttcat gatctgaatg ttgaacggat ccactttgat gccgactttg gtcccgacag
     9121 gaaccatttt cgtggactgt accagccact cgaaaccgtt ggccataact tccatttcgt
     9181 aatggacacc tttgaaaatg gaatgggtca gtgtaccatg gataatacct tcttctggtg
     9241 ctaccagctc cacgtcctca ggacggatga ctacgtcgac gggcttgttt tctccgaaac
     9301 cggtatccac acagtcaaag cgggcaccaa ggatctctac cagacgatct ctgatcatgg
     9361 ttgcaccgat aatgttactg tcaccgatga agtcggcaac gaaagcgttc tccggctcgt
     9421 tgtagatact ttcgggagtg ccgatctgct ggatatagcc ctggttcatg accacgatgg
     9481 tatcggacat ggtcagagcc tcctcctggt cgtgggtgac atagataaag gtaattccca
     9541 actcattttt cagacggatc agttcgtact gcatgtcctg acgcagtttc agatccagtg
     9601 cacccagagg ctcatccaac agcagaagct tgggctcatt tacgatagca cgggcaatgg
     9661 cgatacgctg ctgctgaccg ccgcttaagg aatccggcat acggttctcg aaaccgtcca
     9721 gattgactaa cttcagggca tatttaattt tatcattaat ataagattta cttttaccgc
     9781 tgatctttaa tccgaaagca atgttctccg caatggtcat atgagggaaa agagcatact
     9841 tctggaatac ggtatttaaa ttgcgcttat tgggtggaag gttagtgata tcccggcctt
     9901 cgaaaatgac ctgaccttta tcaggggtct cgaaaccgcc caagatacgg agggttgtag
     9961 tctttccgca gccactgggg cccaacagag tcagaaactc gttctcgcgg atgtacagat
    10021 tgagatcatc cagaaccatc tgattgtcaa atgattttga gatgttcact aaattgacta
    10081 aagcattttt catcaatttt ttccttcctg atgtaaaaaa tcctgcaaaa aatcctcgaa
    10141 aaacctaaat gaaaattttt tgcaagtcga attaatatta tacgtaaata cacggaaagt
    10201 caatgtgaag ttttgcaatt tctggcctaa tttacataaa ctttaccctg tggaaaaaac
    10261 aggagtattt ttcggcaatt ttgcagaaaa aatgtttcac aattttcaga attgtgctat
    10321 atttataaac ataaaaaaag acatagaacg ctaaggagga tccggtcatg agcaaggaaa
    10381 aatatgttgc ctacatttcc agttacacac agggggacag tcatggcatc agaatatatg
    10441 atgtggatat gaaaaacggc cgtttcaagg agaaagataa ggtagagatc acgaactctt
    10501 cctatctgac catttcccac aacggaaagt ttctttattc tataacggat tttggtgtgg
    10561 aggcttatac catccagccg gacggagatc tggagctgat caactttgca ccaatcaatg
    10621 gtatgagagg ctgctatgtt tctacggatt atacggatca gtatctgttt gtagccggtt
    10681 atcatgacgg taagctgaca gtactcagac tgaaagagga cggttccatc ggcgaaatca
    10741 ctgatgaaat ctaccacaag ggtctgggca gtatggcaga gcggaatttc cgtccccata
    10801 tcaactgtgc ccgcatgaca catgataata agtatcttct ggtggcagat cagggtatgg
    10861 atcatgtcaa tgtatatcgt tttgatgtgg agaagggcaa ggtaaagctg gaggatatca
    10921 tccgcagtga gatggaatcc gccccccgtc atatcaagat ttccgaggat ggcaagtata
    10981 tttacattct ccatgaatta aagtgttata ttgatgtata cgaatatgcg gacaataata
    11041 acgaccctac ctttgagaag atccagacgg tagagctgat caagctgacc agaggcaaca
    11101 ccaacagtgc cagcgccttc agtttttcat tggattacaa ttatctgctc agttccattg
    11161 caggggataa ttctgttatc gtatttgatg tagacaagaa gacaggactt ttgaagaaga
    11221 acttcctgct gccgatcagc ggtgagtatc ccaaggatgc agagttcttc ccggacaata
    11281 agtttctggt gtctttgaat catgaaagta atacgatgac cttcttccat attgatctgg
    11341 agcatggaac gatgatcatg aatggaccgg caatgaaggt caatatgccc aactgtattn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggaacgatga tcatgaatgg
    11521 accggcaatg aaggtcaata tgcccaactg tattgtgttc cataagttgg gataagaaaa
    11581 tgatataaaa atcccgcagc ccgcattggg gctaagaaca tcccccgcag cctcggctgg
    11641 ggtaagaaac gcggcacgct ttcgctctgt cgcagagcgt aacggcacat aagcggtcag
    11701 aatacgcccg ctaagtgctg acgtctgctt acggcctgct tattcttaca cccatccggg
    11761 gctgcttgct tattttaata accttgtgat atgagatggg atttggtaaa ataagaacat
    11821 atagaaattg ggaggtagga gtatggcaca gatcagacca tttagggcat atcgtccctg
    11881 tcaggggatg gaggagagga ttgctgcact tccttatgat gtatataaca gggcggaagc
    11941 gtgtgaagtg gtgaagaaga atccggaatc ttttctggcg gttgaccggg cggagacgca
    12001 gtttggcgag gaagtaaaca catatgcgga ctgtgtgtat gagaaagcgg atcagctgct
    12061 tcgggagaaa attcaggaag gaaaatttgt acaggatccg acgccatgtt tctatctgta
    12121 tgaactgacc atggacggga acagccagac cggtgtagtg gcttgtgcct ctattgacga
    12181 ttaccggaac aatgtgatca aaaagcatga aaataccaga gcggataagg aggaagaccg
    12241 tatccgccat gtagatacct gcagtatgca gacggggccc attttcctgg catatcgggc
    12301 gaaagaggaa ctgcagaaca agatcggaga gatcaaaaca cagacaccgg tatacgattt
    12361 tgtcagtgag gacggcatcg aacatcgtgt gtgggtgatt tcggaggctg cggatgtggc
    12421 aatgattgaa gaggcatttg caaagatccc ggctatttat attgcggacg gtcatcatcg
    12481 ttgtgcctca gcggtaaagg tgggattaaa gcgcagagag cagcatccgg attatacggg
    12541 agaagaggaa ttcaattatt tcctgtcggt gattttccca gatgaagaac tgcagatcct
    12601 ggattataac cgggtggtga aggatttaaa cggtatggat gtcgatgcgt tcctggcagc
    12661 catcgaagaa cattttatga tagagaaaaa aggacgtatt ccttaccggc cggagaaaaa
    12721 aggagctttc ggtatgttcc tcgaagatga gtggtactgt cttacagcga agaaggagat
    12781 tcggagcgaa gatgctgtgg aaggactgga tgtatctctt ttacaaaatt acctgttgaa
    12841 tcctgttctg ggaatcgaag atccaaagac cgataagcgg atcgattttg tgggaggtat
    12901 ccggggactt gcggaactgg agcggagagt acataccgat atgaaggtgg cattttccat
    12961 gtatcctact tccatagggg aactttttgc agtggcggat gcgggacgtc tgatgcctcc
    13021 gaagtccacc tggttcgagc cgaagctgcg cagcggtctg tttctgcatg agatagagcg
    13081 gtaataccta cgaccggata agcaaaatag tgacagaata gaatgcagca gactacacgc
    13141 cgcagaaaag agggaatatg acaaacaaac tttataaact gatgaactgg gcaaagatag
    13201 aagaaatcac ttattctgaa tgtgataatc cccatgaact gttaggacct cataagcagg
    13261 gaagtaaaat gctggtacag gcattcttcc cgggagcaga aaaggtgacg atccactgga
    13321 agaagacagg acaggtaggc gaagcttttc tggcagatgt ccctatggaa ctggcagacg
    13381 aagaaggtta tttcgcagcc ctgataaatg ccgggaagat ggcaccttat gaatatttgg
    13441 tggaatatgg aaagacagaa gagcgtgaag cccacagaat gatctgcggc gatgcatacc
    13501 ggcatgtccc gcagatcacg aaggaagata tggatcggtt tgccgcaggc attcattata
    13561 cgatctatga aaagctgggg gcacacccta tggaactgga tggggataac ggcacgtatt
    13621 ttgctgtgtg ggcgccgaat gccatgcgtg tcagcgtagt aggtgatttc aatgaatggg
    13681 acgggcggat ccatcagatg agacgactgg gtgattccgg catttttgaa ctgtttgttc
    13741 cgggggcgga ggtcggagac tgctataaat atgaactgaa gatcaaaggc ggattgacct
    13801 atctcaaagc agacccttac ggtaatgccg cacagaagag accggagacg gcttctgtta
    13861 tcactgactt gcgagacttt tcatgggatg acagtgagtt cttgaaaaaa agggaaagct
    13921 accagtgtgg aaatgctccg gtcagtgtgt atgaaatgta tctgggaagt tttgtgacgc
    13981 cggacgaggg acaggattat gcgaactatc gtgagatcgc tccgaaggtc attgactatg
    14041 taaaaaaaat gggatacacc catgtggaac tgatgccggt tatggaacat cctctggatg
    14101 cttcctgggg atatcaggtg atcggttatt atgcaccgac tgccagatac ggtacaccgg
    14161 aggactttat gttcttcgtg aatgaactgc ataaggcggg aatcggtgtg atccttgact
    14221 gggtgcctgc gcatttccca agggattctt acggtttgtc cgcatttgac ggcacctgtc
    14281 tttatgagca tcaggatccc agacagggca gtcatccgca ctggggcacc ctgatctaca
    14341 attacggtcg tccccaggta tcgaactatc tgatcgccaa tgcattgttc tgggtggaga
    14401 aatatcatgc ggacggtatc cgtatggatg cggtagccag catgctttat ctggattacg
    14461 gcagacagga cggagaatgg atccctaata tgtacggtgg aaatgagaat ctggaagcta
    14521 tcgaaatgat caagcatttt aattctgttc taaaaaagag agacccgggg gtattgtcca
    14581 ttgcggagga aagcacggca tttcccatga tcaccggatc actggatgaa ggcggactgg
    14641 gatttgacct gaagtggaac atgggcttca tgaatgacta tcnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14761 nnnnnnnnnn nnnnnnnnnn nngaaggcgg actgggattt gacctgaagt ggaacatggg
    14821 cttcatgaat gactatctgg attatatcaa atacgatccc tatttccgca gccaccacca
    14881 cggagagctt actttcagca tgatctatgc ctacagtgag aagttcatgc tggtattttc
    14941 ccatgatgaa gtggtacacg gcaagggaac tctgcttggt aaaatgcctg gagacagggc
    15001 acagaagctt gctaatttac gtctgactta cggttatatg atgacccatc ccggcaagaa
    15061 gcttctcttc atgggacagg atctggcgga agagaaagaa tggaacgaaa tgcgtcaggt
    15121 agagtggcag ctggaggaac agaaggaaaa tgccggtgtc cagaaactgg tgaaggatct
    15181 gaatgtactt tacagagaga ataaggcatt gtatgaatgc gatgatcagg tcaaaggctt
    15241 ccagtggatg aacgagatct ctgccaatga atgctatgta agctttgtgc gtaagggaga
    15301 agaggcagag gaactgcttc tggtcgtagc aaacttctcc ggagtaccca gagagatcac
    15361 cacaggtgtg ccttacgagg gaaaatacaa ggagatcctg aacacggatg ctgtgcaata
    15421 tggcggtacc ggtgtggtca atgaccgtgt gaaacgtgcg gaggacgtgg aatgggatga
    15481 caaaaagcag tccgttaccg tgaaaatggc tcccctttcc ttaagcattc tgcagtttat
    15541 tccttatacc gaggcagaac tggaaaaagt gatcgagaag cgtatccgca agaatacacc
    15601 gatccgcaag accgcaggca agactacaaa aagaaaacag gagaaataag gcaacatgga
    15661 tctgacagat aacgtaattg aagaggtgca gaaaaaagaa ggactgatcg catctttcct
    15721 ggataccctg ccggaaaagg cactgaatct tggcatccgg atcggactgg cactggtatt
    15781 ctttttcatt ggaatgcagc tgatcaggct gatccgcaag atcatccgta aatctatgaa
    15841 ccgggcggga gcagagaccg gtgtgatcca gttcgtggac tcttttgtaa aagcttcttt
    15901 atatataata ctgatcctga cactggcgtc ctccttcggc gtggacgcag ccagcattgt
    15961 ggcgttgctg ggatcggccg gcgtggccat cggtctggca gtgcagggaa gtctgtctaa
    16021 tctggcgggc ggtgtgctga ttttattatt gaagccgttt aaggtgggag attacattgt
    16081 ggaaggctcc tccggtaagg aaggtacagt aaaagagatc cagatcttct atacaaagct
    16141 gttaacctat gataatcaga cgattattct tccaaacggc aatctggcnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gttcccgcag atggatgtgc atatgcgtta
    16321 attaaaaata gatattgaat tttacagact ataatgataa tattaccgtt gtaattcaat
    16381 gttttaatat ttacattttt aggaggaaaa aattatgaaa aagtttctgg cacttgcact
    16441 tgcatctgtc atggctgttt ctctggtagc atgcggcagc accaatgctg acaccaatgc
    16501 agaatccagt gcggcagcta ccaccactgc agcaactgca gcaactgcag caactgcaga
    16561 gagcagcagt gaagaacccg gtctgaagat cgctatcgta tccagcccct ccggcgtgga
    16621 tgacggttcc ttcaaccagg acaactacaa cggtgttctg aagttcattg aggagaatcc
    16681 gaatgcaacc gtaaccccca tccgtgagga gaccggtgac agcgcagcag ctgttcaggc
    16741 tgtagctgat gttgtagctg attatgatgt tattgtatgc tgtggtttcc agtttgcagg
    16801 tatcggcaat ctggcacagg agaatccgga taccaagttc atcctgatcg attcctggcc
    16861 taccgttgat ggtaccgaag taaccgcaga tcaggtgatc gacaacgtat acgctgctta
    16921 ctatgctgag caggagagtg gtttctatgc aggtatggct gcagctctgt ctaccaccac
    16981 aggtaaggta gcagtagtaa acggtattgc atatccttcc aacgtgaact accagtatgg
    17041 cttcgagtgc ggtgttaagt atgtgaacgg taccgaaggc atgaacgtag aagtagtaga
    17101 gcttccttct tacgcaggta ccgatgttac cggcgctaac gtaggcggta actatgttgg
    17161 taacttctcc gatgaagcta ccggtaaggt actgggtaat gctctgatcg ctgaaggcgt
    17221 ggacatcctg ttcgtagcag caggtggttc cggtaacggt gtattcaccg ctgcaaagga
    17281 agctgaaggc gttaaggtga tcggttgtga tgttgaccag tatgatgacg gtgcaaacgg
    17341 tgacagcaat atcgttctga ccagtggcct gaaggtaatg catgatactg tttacaacgt
    17401 actgaacgaa gtaaaagacg gttccttcaa aggtgctaat gtgactctga ccgcagcaga
    17461 tgatgctacc ggtttcgtaa gtgaggaagg cagatgccag ctcaccgcag aggctattga
    17521 gaagatcaat gcagcacagg ctctgttaaa ggaaggcaag attgttcctg cggcaaactt
    17581 taacggtgta actccggaga cctttacctg gtaggaagaa tggattcaca agaaggtgat
    17641 ggtgaggaac tgtcaccttc ttcttgtttt aactgctgtt taggcaggaa aatgtacaga
    17701 aatgaggatg aacatgtcgg aatatattgt tgagatgaaa cacatcacca aacgttttcc
    17761 cgggatcgta gccaatgacg atgtgacgat ccagatcaaa aagggagaga tctttgctct
    17821 tctgggagaa aatggtgcag gaaaatccac cttgatgagt atgctgttcg gaatgtacga
    17881 accggatgaa ggagagatct atatccgggg tgaaaaggtg aagctggaat ctcccaatca
    17941 tgccaccaaa ttaaacatcg gtatggtaca ccagcatttc aaactggtat ccaattacac
    18001 cattgctgag aatatcgtaa tgggtatgga gccggtgaag aaggtgctgg gatgtattcc
    18061 ggtagtggat atgaagaagg ccaatcagga tatccggaac ctttcggaaa aatacggact
    18121 ggcagtgaac cctaccgatg tgatcagcga cattaatgtc tctacacagc agagagtaga
    18181 gatcttaaag atgctttaca gagaagcgga gatcctcatt tttgatgaac ccacagcggt
    18241 tctgacaccg caggagatcg atttcctgtt ggatattatc aaatctctgc gggatgacgg
    18301 caagactatc atcctgatca cacataaact ggaagaaatc aaacggatcg cggacagatg
    18361 tgccatcctg aaccgcggac gtctaatgga tgtcatggat gtggctacca cgtccaccaa
    18421 agtcatggcc aacaaaatgg taggccgtga agtgaatttt gagacagaaa aagagcctgc
    18481 aaaaccgggt aagaccatac tcagtatcga gaaactgaat gtttacaata aacaggattt
    18541 ccaggtggta aaggatgtat ctctggaggt acattccggt gagatcgttg cgatcgcagg
    18601 tgtggcaggc aacggacagg tagaaatcgc agatgctatt gcgggactgg taccggtgcg
    18661 ttccggcaag atcctgttaa acggcagaga tattacgaac ctgtctatca gagaacgaac
    18721 gaccgaagga atttcctata ttcccgagga tagacagaat tacggactgg tcatggattt
    18781 taccctttcg gagaatctgg cacttcgtaa ttattacaaa gagccgttct gtcataaggg
    18841 agtactggat cagaagattt ttgaagacaa cagtgaaaaa ctgatagaaa aatacgatat
    18901 ccgaagcggc cagggcagca agacagtggt acgttccatg agtggtggta atcagcagaa
    18961 agccattatt gccagagaag tggaacagga ttcctccctg atgatctttg tgcagcccac
    19021 ccgtggactg gatatcggtg ccatagagaa tatccacaag cagatcgttg cagagagaga
    19081 caagggcaag gcaatccttc tgatctctct ggaattggat gagatcatga attgtgcgga
    19141 taccatcgca gttatttata acggaagcat acagaagatc gccaaagcca gtgaactgac
    19201 caccaatgaa gtgggagagt tcatgatggg cgtaaaaaac aaggagaagg aggcataaga
    19261 ccatgaaaaa atttacaaaa ttatggaatg cggtaaagat tcctctcctt gcgatccttt
    19321 gtagtcttgt ggcaggtggt gtgatcatcg ctgtgaccgg ttccaatccc tttaaggctt
    19381 attacgcact tctacaggga agcggactgg cacccaagtc ctcctacgcc agctacaaga
    19441 gtatgctgac ggactttatg agttatgtga actattttac ccccatgatc ttcgcggcac
    19501 tggctgtggc ggtagcactc cgcgccggac tgtttaatat cggcgtctcc ggacagatgc
    19561 tggcagccgg atttaccgca agcatcgttg tgggctacag cagtctgaac gctgtgctgg
    19621 caaagccgct ggtagtgatc atcggcctga tcgtcggtgg tctggcgggg gcactgatcg
    19681 gcttcctgaa ataccggttt aatatcaatg aggtcgtttc ctctattatg ttaaactata
    19741 cgttccagta tgtgatcagt ttcttcatca ataccttttt cgtggatcct gtctccagac
    19801 agtcaaagga aatctccgca gcttccagac tgacactgat ggataccatg gtgggcaatc
    19861 tgaaaatgga tattccgctg ggaattattc tggctctgct tacggtggtg gctgtatggt
    19921 tcctgttaga gaagacaaag cttggttatg aactgaaggc tgtgggctat tcccgcagtg
    19981 ctgcaaaata tgcaggtatc aaggtaggca gaagcatggt actgtccatg acgatttccg
    20041 gtgcactggc cggacttgcc ggtgtgactt attatctggg ctatgtggga tccattcagc
    20101 caaaggtatt gatcagtacc ggatttgatg ccattgcagt atccttgctt ggtaacaaca
    20161 atcccttcgg tattgtattt tctactatac tgatcaccct gatcagcaag ggaagtactt
    20221 atatgaaatc tgcggccggc gtggatgcgg agattgcatc cgttatcacc ggtatcatcc
    20281 ttttgttcag cgcatgtaat gcttacatca agtggaaaat ggagcgaaac agaagagaga
    20341 aaaccaacaa gacaaaggag gacaaataaa tggatattgt gatcattgac ggtttatcct
    20401 ttgcactgcc tttatttatt atggccatcg gtggcatcta ctgtgagaaa tccggtgtga
    20461 ccatgctggc ggtagaaggc ttacagggtt ttggcgcctt tatcggtgca ttcgtggcgg
    20521 ttgccattgc gggaaacttt gccggagact ctccggcgcc tttctacatt gccatggtta
    20581 tggcggctgt gggcggaagt ctgtttgcac tgattcatgc cctgttatgt ctgaagttca
    20641 aggccaatca ggttatcagt ggtgtggtag tcaatattct ggccatggcg ctgaccgcat
    20701 atttaacgaa gctgttcaac agagtggtat ttggtgctac gtccgataag tttgtactga
    20761 ctgtttcttc cagaattacc atcccgggaa tctctaagat tccggtactg ggagcggtat
    20821 ttaccaatct gtatcctttt gaactggtga ttgtggtagt ggctttcgtg gcgtggtttg
    20881 tgatgtataa gacccgcttc ggcatgcatc tgcgtgcctg cggtgacaat ccccatgcag
    20941 tggatgcggc cggaagacag gtgggacaga tccgtctggc agccattatg atctgtggtg
    21001 cgttgtcggg actgggaggt atctgtttcg cgtattccat ttccgcgaac ttttcctcca
    21061 gtatttatgt aggttacggt tatctggcta ttgcggcact gatcttcggc aactggcgta
    21121 tcctgccgac tctgggagcg tgtctgttgt tcggatttgc cagatccggt ggataccgtc
    21181 tggtacaggt actgcagatg cccagcagtt atcaggatct ggtcatgatc ctgccttatg
    21241 tactgacttt gctgcttctg gtattcttct ccaaacagaa cggtgcaccg agagcactgg
    21301 gagtgatcta cgacaaaggt gcaagataag cagacatttg cagaacgtag ctttgcatgg
    21361 ctctgaagag ttactacaaa aaaag
//
LOCUS       DAWEJW010000047        21202 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_981_length_21202_cov_61.586088, whole genome shotgun sequence.
ACCESSION   DAWEJW010000047 DAWEJW010000000
VERSION     DAWEJW010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 21202)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21202)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21202
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_981_length_21202_cov_61.586088"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5656..5755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4835 a         5812 c         4964 g         5491 t
ORIGIN      
        1 cagcggcaac tctgatatct tatcataacc gctttcgttt gtcaacaact ttttttgaaa
       61 tctttttcgt cttggaaaac tttgtttttc cgtgacaggt atttatgtta acatatctgt
      121 cagactttgt caacaacttt tttgaaatct tttcaagttt ctttcgagct gttgtgttgt
      181 ctcggcgttg cctcaacaac gaatctgagt atatcatccg ctagtgtact tgtcaacact
      241 attttttgaa ttttttgcac aaattatccc ctgctacgtc aattccgcat aaacagggga
      301 tttttgccat gtttctattc tgttttttac tattttacaa agaacggaag cttttgtccg
      361 agctgttcca ccagatatgc cagatagtta ttttcataca aacatgtaag tattctgctg
      421 tctctggtat aatctacgat cacctggccc agggttccca ggtctctgaa gatattaaat
      481 gcgtatattg tccaaacaat tagaacagtt tctaagattc caaccacaat acctagtaat
      541 ttgtccaggc tatgtactat aggtagtttc acgatcatct ttgccgagaa aaatacgata
      601 ttaagaagat gatgcaccaa accgattgcc gccagcaaca gtattgtcag gatcacattg
      661 aatatctttc catcaaagta actgctcaga ccattcccga tcaggaacag caccacacag
      721 gtaatcagca gggagatcag agagatcaga gattttacca ttcccttctt gtagccgtcc
      781 atcaccatgc agatcaatac tactgccatg atgaccagta ataaattcag attcattggt
      841 atactccttt attttaactg tatcgtatct attgtactat ctgtcaccag ataatatttg
      901 taggaacctc ctgcaggaag cagaagccgg gcactcttac cgaagctgcc atggtatttt
      961 tccacaccgt ccgttgtaaa gatctggcac tccgattcgt tgtagatcac catgtcatca
     1021 tccccgaaga aaatatccgt ataatccaca tcaaaatagt agctgcccac ctttgctgcc
     1081 gaagtattgt acacatccat gcggtacttg gtctccgcct ggtccgaaag gaataccagt
     1141 cctacatatt tatcactgta gaaaaccgac tggatctcgt cgtcatacag gtattctccc
     1201 gccgtcacag gcttctgggt acccgagtag atcataagac ggctgtctcc taccgcaaac
     1261 gctgtatcat tattcataaa ctgcactttc ggcacgacca ggtcggaata agaatatgcg
     1321 ctgaccatat aatcgctctg attctcgccc accggtccga aattgtaaaa tgtcacatta
     1381 ctttttacca cgcctgcatc cacatacagg taactgatcg ccagaagctc tccgtccggt
     1441 gaaaggctga tggataccgg ataccctccg tccgtcatat gtgcctgtcc cgtatagctg
     1501 ttggtcccgt ccgcttcgta agtcatgatc caggctacat ccgtatctgc cagcaccgct
     1561 gtcactcttc ccgtttccgc tactgtgata tttcggatcg gcatggtcgt tgtaatagtt
     1621 cccagttgct tgtccgtatt ctgcacgtag atctcacggc cgttataact gccgatagct
     1681 gccacatcgc cacaagtagc gatcttcaca tcctggatca cataagtctg attccacttt
     1741 acatttccct tggcatccgt acagtgagcg ccgtctttac tatatgtaag aatactgttt
     1801 ttcaaccgta tgtctgtggc atccgatgag acctcccggc taatggaaga tacggtgtca
     1861 tatcccgtat agatatgtct ctttgcctgt accaccacaa gagcaagtgc cgctcccaaa
     1921 gccgccacca ccaacagcac ccggtacacg attttcagcc ggtgtttacg gatctttttc
     1981 ttatagtcat cctgactctt ttgccgcttt tctttttctt tcagataatt cttgatatct
     2041 gctgccatgt atctgtttcc ttatgtatta tcgtaactat taagaatcgc attcgcaaaa
     2101 ttactgcggc gcagtctgct ttttgctcat gtagttacag tataacacac ttcccctttt
     2161 tgaaaataag ttttttacgg gagaaggatt tcctgaccga tcttgatatc atcggggtca
     2221 gcaataccgt tcagggaaca gatctcagat actttactgt tgctgccgta gttccgcaga
     2281 gaaataccga tcagtgtgtc ccctctctgg atagtataat gtgtgggttg agcaaccgcc
     2341 tccactgtag gagtctctgc cactgcagat tctgccgtct gtgtcacatc ctgtattgtt
     2401 tcctgtgtcg tgttctgttc tgttgcctcc gtcacatctg tcgtattttt tgtgacattg
     2461 ccgtccgcag atgttccatc cgctacactg cccgtatttt cttccgtcgg atctacagca
     2521 ctgtttcccg ttccatccat tgatgcattt tccgtgcctt ccggcatcgc tgtctctgct
     2581 gcagccccag tagtatttcc cgccgcagca ttttcctgtc gcagagcatc ctccaacttg
     2641 tcctccgcca ccagcgtgct gatctggctc tgttcctcca ccgcatccgg cagtttctgc
     2701 tccatcaggt tgctcatggc ctgcctggct gtctccccca gggattctcc ggtgctttcc
     2761 tgccgcatca atgccaatgc catcacgcag agcagaacaa agacaccggc agccgccatt
     2821 ttcatccgac gcagtccggt cgttggcatg ggtataatat tatccggttc tctgcgtctg
     2881 ttctctaccg ctctgcgata ttccacaggc gcattctccg tatgtccggc ggcacggaag
     2941 gcatttccgc tctcctgtct ctgtctacgt tcctcctgcc gtttcttcac cacttcgtat
     3001 ttttcctgat ccaccttctc cggtgccgcc tcttcgcccc ggttctccag catatatgcc
     3061 agcatactgt cattcttctc atagaactgc gcataccccg acacataacg gcagatctcc
     3121 tgttcgcaga tctggaagcc ttccaccatc tcgccattca gtatctggta gcctaaaaag
     3181 gtcatttccg aaaaatactt tctccgcagc ttctcaatct cctgttcctg cgcctgggac
     3241 aggtgccgca ccggcttctg caggaaattc agctttgcgg agccataggc gaatagatat
     3301 gccacaccgt cctctgctgt cttcctgccg tataatgcta tggcaatatc cttgttctgt
     3361 gccattccat tcatttgttt tatgtaagat accacataat cttccacata gattctgcag
     3421 tgcttgtccg cctctccgat ctgcgttatg ttcttcggta attccataca aaaaacacct
     3481 cccatgccgt ttctatcagt tatcactaac tatagcatcc gcacaggagg tattttagcg
     3541 ttctttgtcg cacctcttca aaaacagttc gacaaagttt gcaccatatt ctatgtattc
     3601 ttagctatgc ttcaaactta tatacggtaa cagaatcgta ttctctgccg ttctccccgc
     3661 caaatacata ggaggggaaa ttctcatgat gaatggtatc cgggaaatac tgggtctcca
     3721 atgcaaaacc gcagcggttg ccgtaggtca ccccatcttt tccgggctgt acatcaataa
     3781 aattgcctgc ataaaactgc actcctggca gggtggtgta tgccttcatt actctgccgc
     3841 tcttcgggcc ttttactgtg gcaatatgcc gtaatgtacc gtcatcgttc catccatcga
     3901 tcacgaaatt atggtcatag cctccggtca ggagtaactg ctcataatct tcccggatgt
     3961 ctctgccgat cttcttgggc tgtgtaaaat ccatgggtgt gcctgctacc gcacgaatct
     4021 caccggtagg gatcgatcct gctgccaccg gagtaaagtg agaggctctc agccacagtt
     4081 catgctcttc aatgctgcct gcaccgtgac cgtccagatt gaaataggaa tggttggtca
     4141 ggttgataat ggtcttcttg tcactggaag catggtaatg cagagtcagc tcattctctt
     4201 cactcagaga gtaggttacg gataccttct tattgcctgg aaatcccagc tgcccatcct
     4261 catcctccag agaaaatgtc acgctgttct ctcctatctc cgcatcccag agtctcttat
     4321 ggaatcccag ttgcttatgg gtatgtaaat tgttggcacc gtcatttttg tccagctgat
     4381 aagtaacgcc atccagcgta tactctgcac cgccggtacg gttcgcacag gggccgatca
     4441 ccgtgccgaa aaagctgtcg ttgccgtaat agctctcacc attgtcaaat cccagcacca
     4501 ggtcgtccac cttgcccgtg gcatccggta ccagtacgcg caccagagta gcgcccagat
     4561 ccgttatctc tgctttcatc ccttgatcat tactcagggt atacagagat atctctttcc
     4621 cttccgcact tttcccaaat aaacttttta ttactgccat tgtcttgtcc tccctctttt
     4681 aactataatt ctactctacc ctctaaggca cggagtaatg ttacctcatc aatatattcc
     4741 agatctcctc ctacagggac accgctggca attctcgtca ccttgattcc tgtaggtttg
     4801 atcagcttgc tgatatacat tgccgtggtc tcaccttcca gactggagtt ggttgcgata
     4861 atgacctcct ccacatcccc ctgtaagcgt tccatcagtt ccttcagctt gatatcaccc
     4921 ggtcctacgc ccagcatcgg agaaatggcg ccatgcagta catggtacac gccctcgtat
     4981 ttgccggtct tctcataggc cgccagatct ctggtgttct ccaccaccat aatggtacga
     5041 tgatcccgtc tgggattcgc acacaccggg cagagttcct tatctgtcag ggtataacac
     5101 tccttacagt accggacatt ggctctggcc tcgatcatga cattcgccag tctgtccaca
     5161 cggtccttcg gcatattgat caggtgaaaa gccagtcgtt gtgcggactt actgccaatc
     5221 cccggaaggg ctgccagttc ttctattaat ttattgatct gtccgctgta gagttccatt
     5281 agaaaggaaa tcctcccagg ccgccggtca ttttgcccat aagctctgca ttggcttcat
     5341 ccgccttgcg cagtgcttcg tttgctgctg ccacgatagc atcctgcaga gtctcgatat
     5401 cctcgggatc cacgatctcc tctgccagct tgatggatgt gatctctttt ttaccgttga
     5461 tggtcacttc cacagctccg ccgcctgcag atgcagtgaa ctccttggtc tccagttcct
     5521 tctgtccttc ttccatctga cgctgcattc tctgtgcctg cttcatcaga ttattcatat
     5581 ttccgggcat tgcgcctccg ggaaatccac ctctttttgc catgttgctg tttcctccat
     5641 attatttata attgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctgcc
     5761 gcaatcccaa attcacgcag gatcactctt cctcgtcgat ctccatgttg atgaccttgc
     5821 ccagatccac ataattgtct tcgaactccc tgctgctttc cacggtctgg atcgtcactt
     5881 cgatctcctt accggagaaa tcggacaaca gcatttccaa ctgctgcttg ttctgctcgt
     5941 gagcttctcc tttgaaataa tcggaagcca gtccgtcctc caacaccacc atgagcttgt
     6001 tgtctccacc cagacttaat cgggcgtttt tcagatacat cttcatggga ttctcggcat
     6061 ttcccacgat ggccggccat ttccgtacga tctcctggat atccccgggg accgccttcg
     6121 gcaactgtgg cttcggcttt gccacaccgg cctgcggcac tccctgtgct ccgggagctc
     6181 cggcaggcat ttgcgccgcc gtaacgacaa tgccgttttc cactttttcc tccacctggc
     6241 gaatacgatc caggacagct tcctgatccg tctccatctc cggcttacac agcttgatca
     6301 gcgcgatctc taccaggata cgcttctgcg ccgcatagcg gatctgtccg gacagttcgg
     6361 agaaaatatg aatatagcgg atgatctggt ccatggacag catggaagct tcttccttca
     6421 gattcgccag attgtccgta gacatgtcta tgacatcctc cagattatcc gctgtctgta
     6481 ccaacatcag attccgcaga taccaggtaa agtccgtgac gaactgtacc agctccctgc
     6541 cctgcatgac gatctcttcc aacagttcaa tgcagcctaa cacattccgg tccataacga
     6601 agcgcagcaa tctgctgaac acttctgtat ccactgcccc cagcacatcc agggtcatat
     6661 cgtaggtcag ttctttgccc agatggaagg caatacactg atccaacagg ctcaaggcat
     6721 ctcgcataga accgtccgct acttttgcaa tgtagcgcag agctctgtcc tccacctgta
     6781 cctgctcggt gtccatcagt tccttcatac gatccgtgat ggtatcaatg gagattcgtt
     6841 taaaatcata tctctggcag cgggacagga tcgtaatggg gatcttgtgc acctctgtgg
     6901 tcgccagaat aaaaataaca taagagggcg gctcttccag cgtttttaac aatgcgttga
     6961 aagctccggg ggagagcata tgcacctcgt cgatgatgta gaccttgtat tttccctccg
     7021 cgggggaata ggatacctcg tcgatgatct cacggatatt gtccacaccg ttgttggaag
     7081 ccgcatcgat ctcgatgaca ttcatgctgg ctcctgctgc gattgcctta caaatacggc
     7141 attccccaca ggggccgtcc tctgtggggt gttcgcagtt cactgttttc gcaaaaatct
     7201 tggccaccgt agtcttaccg gtacctcttg ttccggtaaa caggtacgca tggccgatcc
     7261 ggttcgctcg cagctgattc ttcaatgtcg tcacgatatg atcctgaccc ttgacatcgg
     7321 aaaaagcgtc cgggcggaat ttccggtata gtgctgtata tgacatgttt tttctctctt
     7381 ccttgtaact attcagagcc atcaaatatt agcagatgct tggcaaaaat agttactcat
     7441 taattttctg tcttaatcgc cgaagcagat gcccagcagc ttcgcttcct gtacgatggt
     7501 agcatcggga tccacggtct ttaacttgcc cgctacatcc tctaacggaa cccttacgat
     7561 ctcacggttc ttgtaaccta ccatgaatcc atactgatta tccaggatca gcttgcctgc
     7621 ctcggagccc agacggctgg cgaatactct gtcataaggg caggggcttc cgcctctctg
     7681 catatggccg ggtacggtga cacgtacttc tctgccggtc ttctcctgga tctgtgcagc
     7741 cacctcgtag gatacggaag ggaacggata tttttccatc tttttcttgt aatctttttt
     7801 ggtcagggca gcatcttcct tggaaatcgc accctcagcc acagcgataa tggtaaaacg
     7861 gcagcccttt ttgtccctgt cttcgatctt ctcgatcacc ttatcgatat cataagggat
     7921 ctccgggagc aggatgatgt ccgcaccgcc tgccattccg gcattcaggg tcagccaccc
     7981 tactttatgt cccataactt ccacgataaa tactctgccg tgggaagctg ctgtggtatg
     8041 gatgcagtcg atggcatccg tagcaatatc tactgcgctc tggaaaccga aggtcatatc
     8101 agtaccccac aggtcattgt cgatggtctt gggcagggtc acgatattca gtccttcctt
     8161 gcgcagcagg ttcgcagtct tgtgggtgcc gttaccaccg agaattacaa ggcagtccag
     8221 ctgcagcttg taatagttct gcttcatggc ttccactttg ttcagcccgt tgggatccgg
     8281 atcctggatc gtcttgaacg gggtccggga ggttcccagg atcgtgccgc ccttggtaag
     8341 gatgccggag aaatccttgt ctgtcagcat gcggaaatta ccgtaaatca gtcctctgta
     8401 accgtccaga aaaccataga tttcaacctc ttccttgccg ttcgccaggg tcttcaccac
     8461 gcctctcatg gctgcgttca atgcctgaca gtcaccaccg ctcgttaaca ttccgattct
     8521 cttcatcgta ggatcctccg tttctatatt tcattatttt ctcttgcgtc tgctataact
     8581 atccaacact ttgaatcgtt atttctattt taatacattt gttttcatct gaaaatagtt
     8641 tgttgtaatt tccgaaagat ttcgatatcc tgcacacgta aaaaacgcct gcgcttgcca
     8701 ggcgtgggcg ttataaaaag gagaagacgt tatttagtat tttgcaaaac gtaaaatcaa
     8761 aaatggcata ataagtctat tagcagtatt tctcaattat cttctgcata gcatcccagt
     8821 tttcttccat atctgcagtc agcttaaact gcgtccgagc gcggttgagg ttctgctgtt
     8881 cgtagttgat gtggaaataa tggtcgccct ccagataatc ggcaagaaag cgcataccgc
     8941 actccatcgt catcagcttg gcaccccaag gcagggtctg cttttccaat gtagtaaatg
     9001 cttctcctgc agcttgcaaa aagcctttcg tgtaaatttc aaacagttcc atgctgaaat
     9061 tgaccttcga gagatcggct tcgtcctcgg cgcagtggtt ggcaccgaag cggatggaat
     9121 cgccgaaatc gttggcggcc agacccggca tgacggtatc gaggtcgata acgcaaaggc
     9181 cctcgccggt cgttttatcg aacaggatgt tgttcagctt ggtatcattg tgggtgacgc
     9241 gcagcggcag cttgccagcc gccagcaggt ccaccatgac atggtaatct gcttcgtgag
     9301 cgcgaacaaa ctcgatctca cgctgcacct gtgctacgcg gcccatacga tcctcggcta
     9361 cagcgcactg aaagtcagcg aagcgctttg gtgtatcatg gaagtgagga atcgtctcat
     9421 gcaaggtatt tgccgggtag tcggcaagct ggtgttgaaa atcaccaaaa gctacggcgc
     9481 tttggtaaaa attctcggcg gattgcacct gctgcaagca gacggtgttc tctacaaaat
     9541 catagatgcg ccagcagctg ccatcggtgg cccggtaata aggctttcca gctttggtca
     9601 gcacaacatg gagcgtggcg cgtgccgggt cattcccaca ctgtttggcc ttttcgcgca
     9661 gaaaatccgt tacgccgcaa atgttttcca tcagaccgtc agggtccttg aatgtatcag
     9721 tattgatctt ttgcaggatg tagcgctttg ccccctgcgc agacgttacc ataatggcat
     9781 acgtttgatt gatatgccct tcgccatagg gaacaggctc tgacaccgag ccattccatt
     9841 gccatgcagc caaaatttca ttcatcataa aatatcctct caggtttcgt ttatgccgcg
     9901 atgctgtaca acacgacgcc ggacacggcg gcccccgcca tgaccttgat ggggctggga
     9961 ttccacttgc gcaaaataaa aaagcccaca aggaaaatag ctactgcaat caaatccata
    10021 ctgccaaact ccgcagggaa agtgcgctgc ccgtacaggg ccatgcataa cagggagata
    10081 ccggcggagg cgatcatagc aatgacggcg gggcgtagcc cgatcaagat gccctgcata
    10141 acgctaaggc cgcggaaacg gtaatacatg taggccagcg ccatgacgat gacgcaggac
    10201 gggaaaatac agccaatcgt ggcaatcagt gccccgggca gccccgccac ctgaatgcca
    10261 acaaacgtcg cagcgttcac cgcgatgggg ccgggcgtca tttcggaaat ggccatgatg
    10321 tccgcaaagc ctgtcatcgt cagccagggg tgaatatcca ccacttgatt ctgaatcaag
    10381 ggcatagccg catagccgcc gccgatgctg aacaggccaa cttggaaaaa gctccaaaac
    10441 agttcaaggt agatcatttg tcgccctccc gcttttgcag atacagggta tccagcgcac
    10501 cgatgacacc acagaccaga ataatcagga tgatgttcac cgagaagaag cgggtcgcca
    10561 caaaagccgc cagcatgacc gtaatgggaa gccagcgttt ctttttcaga ataggccaca
    10621 tcatattgat gacgacgtca aagatgactg ccgccacccc ggccagcatg ccggccatcg
    10681 ccatgctcac gtacttattg gagcggaacg cctgatagaa cacggagatg atggaaatga
    10741 tgataagcgg cggcagtgct gcacccacca cagtaatcag tgcgccgggg atacctgcca
    10801 catggtaacc taccaaaata gaggcattga tggcgatgga gccggggctg gattgggcaa
    10861 tcgccgttag atccagcatt tcttcctcgt caatccaatg tagttctttc acgaagcgct
    10921 cccgcatcaa agggataatg acgaagccgc cgccgaaggt gcaggcgctc agcttaaagg
    10981 tagagagaaa cagctgccag tatttatttt tcttgtccaa tagaatcggt ctccttttca
    11041 gtgatacatt tgaacagcgc ggatcatttc gtacaggcgc ggttccgtgt aggcgaaaaa
    11101 cttttgtagg gcgttgcaat agcaactgcg ctgcgctgca gctgccgtat ttcggtcgcg
    11161 cttgcagcca ccacggcaca gccgtaccca tttgcattgc tgacattctg caggtatctg
    11221 ccagccatcc gtgataaacg ttcgcatttt ttcgccggta agcagcgatt gcagggtatc
    11281 gttttgaatc gtaccaagtt tataggcatc caagcagaag aagtcgcacg gatacactgc
    11341 gccactctcc tccacgacca tgtagacacc gcaggttccg gtcgtggaac aagttcccgc
    11401 cgggccaccc aaaagtagct gaatataatc ttcaaacagg cggatgctga cataggtgat
    11461 ggttctccag tcgcggtacc atgcgtcgaa cagtgcgcac aggaactgcg catacagttc
    11521 cggtgtgagg gagtagggca tcatgcacat ccggggtgcc gtccaagctg atacctacga
    11581 gaaagtgttc tttgctgaaa aatcagccca tccatcggta atggcaagac catttgtctg
    11641 gatagcgtat tgcacggcaa cgtgcttttg gttgtacttt cgcaccgtct tcgtaaagaa
    11701 acaaaagaaa tccagtccgg tcagcgttgg ctcgccgccc tgaaaggcga acgaaacaaa
    11761 gccgcccggc tcaagttccg caaagcagcg ttaaatcagc gtttccgtcg ttgcctgtga
    11821 catcatacag tcgtcggggt tcacgcaatt ctgctgctca tctcggtaaa agcaatattg
    11881 gcagcgcatg ttgcagtagc ccgctgcggg tttaatcagg aattgaagct gcttcatagc
    11941 acctcggtat gctgtaatac ggtcacgggt gttttgccca caagcagggt gtacccatcg
    12001 gtgtaaccct cttcacggtt ttttagagca tcaatcagca gagtgcgcag cgccgtcacg
    12061 cgctctgggt tttcctccag cacgttcttc gtctcattcg ggtcgtcgcg caggttgaag
    12121 tacagctctc gcccggtctg agagtaccag atatatttat cctttttcgt caggataaag
    12181 tgcatggaat cctcgcccag tgtatgctca ccgtgcagat attcgcgttc gacggggtgc
    12241 aacaggctcg tgccgtccac ggtttcggga atcggtgcgc ccgccagctc cagcagcgtg
    12301 ggcatgacgt cgcgcagttc tgccaaatca gtgcaaaccg taccatgctt gcccacatag
    12361 cgctccggtc cggagataaa cagcggcacc cggatgctgc cctggaacgg ctttgctttc
    12421 tggaacatca ggtggtcgcc cagcatctcg ccgtggtcgg acacgaacag aattatggta
    12481 ttatccatga tttgctcctc caccagtgcg gagactaaac gcccgatttg atgatccatg
    12541 tgcgtgatgg ccgcatagta acccgcccgc aactggtgga tgtagctttc atccgaaggt
    12601 gccgtctggg catggtagct gtggctgtca cgttcccagc ggacgcagtc gttccaatca
    12661 ccgcgccaag gctcggccag tgactcatct ttgtacaaat tcaagtaata ctccggggca
    12721 tccagcggag gatgtggccg cacaaagctg gccatcagga agaatggctt cgagcggtca
    12781 cggcagcgca gaaagtctcg gcactcgctg gcgacccagt tggttgggtg atacttttcc
    12841 tcgtagatcc aggggcgcac gacccagcta ttgcagtcaa gaccagtgtc caccgggtct
    12901 gcatccacgc caagctgctg tttgagccac caataatagt catcggccac acgctgatcc
    12961 tcatagctgg gcgtggtggg gcggcggtag gcatggagat agccatcatg cagccgtaca
    13021 tcatggaaac ccaggttgtt gcgcagcgga tgaacatgca ttttgccgac acactgggta
    13081 tagtacccgg ccttgctcag ctccccggcc agtgtgtggg gataatccca cggcacaaga
    13141 tcctcgtacc cgacgcgacc cgtgtgctcc tgggacatgc ctgtatacag cgtagcccgc
    13201 gcgggaacac aggtcggaca ggcagaatag gcattttcat accgcacgcc ctcggcggcc
    13261 agtgcatcga ggaacggcgt ttttacatcc gggtgaccgg caataccaag gcagtcgccg
    13321 cgcatctggt ctgccataag caggataata ttaggatgtt tatccataac aaacctcgta
    13381 tcaatgtttt tgcagccaaa gggtgccgca cagtgcggca cacccttgtc ctgctttata
    13441 gattatacca tttgatctcg ttggcgtcca tgtgcagggt aaagctgctg ccgtcgccag
    13501 tgtaaacagt ggtatcctgc ggctcgtagg tgttattcac gacacagtac ttgccgttct
    13561 tgacataggc atgtacttcg acattgtagt ttgtgctaaa ccacttgtgc aggtcggcct
    13621 cgtcgtgggc ggcccacagg atggctcggt acagcacgcg gttgttcacg aagctgtagg
    13681 gcagaccgct gatgtagacg ccgcgaccct gaccgtactc gtgggcggcc agctggacct
    13741 ccttatcacg ctgaatcaaa atctcggtgc cctcaagcgc gaagatgttc tttttgccct
    13801 cgccgaagtc cacatcatgg tcggggcagt cggccagaat gaagtgatcg cggtgctcgt
    13861 cccagttgta cttgtcgtag ttcagggtga agcccgtttc tttttctaca ccaagcggcg
    13921 cggccagctg caaataccgg ccctgatact ggtggccgcc gggttcgccg acgccgatca
    13981 gaccgccgcc acggtggaca aagcccttga cagcggagga aatctccgga tcctcccaga
    14041 ccttgccgcc ggtatgggcg gtatcgccgt cgcctacgtt gataaggaca tcaatgctgt
    14101 ccagtacctt cgggtcgacc ttgatgtcgt caaagctgat gaacttcaca tcaaacggcg
    14161 caccggacag agcctcgata acacccgcat agctgtagtt ctgcttctgg tacagtgcat
    14221 ggtgaaccat gtggcagccc caggcgcgct gttggcccca gctgttaagc acagccacgg
    14281 tcttgacgca gtagggcgtg gtgcctttga tgttctcgta cagctcgcgg aactcgttgc
    14341 agacgctctc gacataatcg agaaattccg ggaactcgca ggccagcttc aggtagccgc
    14401 cgtagccgat acgatcaatg ggcttgcgca ggatggcacg gcgagccgtg acccagttca
    14461 ccttggcctc gcgcacgggg tcgccgccct cgtggaaggt atcggggaag aagtagggca
    14521 ggaagcgtcc ctcggtgtac ttgacgccgg gaatatcgga aatcaggcgc agcgtgctgc
    14581 cgttgccaac actgccgacg atagcatcca cgccggactt ttgaaactcg ggcatgaacg
    14641 gctcgcagcc gatccagtgg tcgccaagaa acatcatggc ttccttggcg taggcgtgga
    14701 caatgtcggt catttctttc atcagaccgg cgacctcgcg gcgctggaaa gcctggaagt
    14761 ccttgtactc cttgctgggc acacggtact ggttgttgta gtagccctgg tcgatgataa
    14821 actccgggcg gaacttatag ccgacctctt tttcaaactg ctccaaaatg taggggctga
    14881 cagaggccga gtagccatac caatccacgt acttctcgcg gcgcagctcg tcaaacacca
    14941 gcgtgaacag gtggaagaag gtcgtaaagc gcacgacatt gacataggga tggctttcca
    15001 gatactcacg taggcggcgt agggtgtagg cgtgggtctt gggctggcgc acatcgaacg
    15061 ggatctggtg ctcgacatcc ttccagtcgt tgatgaccga attgtacatg tggacgggat
    15121 cccagatcag gtacgccaaa aagctgaccg tgtactcgtg gtaggcggcg ggggcgtcga
    15181 tgacaacgct ttcgctggcg gcatcatagt gccagtcggc ggtggggaca ggctcgcccg
    15241 tcgtgcggtc gatgacctcc cacaaacggg tgatgtcatc gtggtcgttg accatcatca
    15301 gttcgcggct gatgcccgtc atcaggggga tggtcagcgc atcgtccgca gcggtgtaga
    15361 acggcgtcat aatgtagcac tgctgcgttt catcgggatt ggcttttgcc catgcgttgt
    15421 ccttgcgggt ggtgtagtag gtggcataaa tcttggcgcc agtgtccttc aactcctgcg
    15481 ggaactcggt gccgtcgcag tcgcggatgg catccgcgcc caagcgcttg agcaggtcga
    15541 gggtttcagg gacggcgtcc atatcggtgg ggatcgttac acgacccatt tttcttacat
    15601 cattcatata atgattctcc ttaaaacgta atattttgaa tggaggaaag tcaaatcaac
    15661 ccttaacgcc gccactggta gccccgacaa taatcttttc agacaggaaa agatacagca
    15721 ggaaagtagg caggaacaca atgataactg cggcaaacat agcaccgtac tggccggaat
    15781 acttcattgc attgacaatc tgcaacaaac cgacaccaac aggggtcata gaatcgctgg
    15841 tggtgaaaat cagcgccatg aagaactcgt tccacacaga catgaagttg aaaatcgtaa
    15901 cggtaataat ggcgggctga accagtggca gcataatctg ccagaacgtc cgagaatggc
    15961 tgcatccgtc tatgtaggcc gcctcctcat aagaccggga cagtgatgag aagaaatcca
    16021 gcaggtaaat cgtggtatac ggtacgcgca gcataatata cagcacaacc aacagcacgc
    16081 gacccttgat gccccactgc acagccaaag agtacagtgg catgatgatc ataatagtgg
    16141 gaacactcat ggcaatgacc aagccgatac ggatggtctt gccgccgacg aacttccagc
    16201 gggaaagcac ataagcacca ggcgcagaaa tcagcagcac gcccacacag gaaacaatgg
    16261 cataaaccaa gctattgaag aaatacagcg atacattttg ggtgttccaa gccgtaacgt
    16321 agttatccca cttcaggcca ttggaaaagt ccataactct gcccgtcaga atttcctttg
    16381 ttgaggaaag gctggcgcag acgatccata acaggatggc agccgtgagt gcgatccatg
    16441 cgcaaacaat gatataaccg ggcagaagcc gaagctcttt cttccagttg attctatcgt
    16501 gatattttcc cataaccgct ctcctacctt aatactccag atccgaatct ttcaagatca
    16561 gattggtgat aacatacaca gcaatgataa tcaaagtcag cacaacgcca accgctgcgc
    16621 ccgcaccagc atcgcgctcg acaacgccct tgccgccgaa cactgtatcg tacaggtaca
    16681 caacgggcac aatggtagcc gtttctgtat tgatggggga gaacatcttc gtccacagga
    16741 agaatgtagt cgcattgatg ctccagaaag taacgcaggt gcgaatcaca cctttcagca
    16801 ggggcagcgt aatctggaag aagcggtcaa agatattcgc gccgtcgatg gtggcagctt
    16861 cgtataaatc ctgcgggata ctttcgatgc cgctgatgaa aatcagcata tagtaaccaa
    16921 cgctgccaaa aatgaaggca atcatcatgg cccagaaaat cagatctgta cccagccagc
    16981 ggacattctc accgccgaat actttgacga tctggttcag tagaccataa tcatagttga
    17041 acacatactg aatccacatg gtagccagcg ccacggcact aatgatgttg ggcatataaa
    17101 tggcggcctt aaagaatttt gcgccgcgga tgccgctggt caaaatcacg gccatcagca
    17161 tagacaggca catggtaatg atgccgccca ccagccaaat catcaccata tttttcatgg
    17221 aactgataaa gccggcatta cgcaggattt tgaaatagtt ttgcagccca tagaagctcc
    17281 atgtacttgt agatgccgta aggctctcta ccttaaagaa actcatgata atcgtccgaa
    17341 cgaccggata gacatacatg aacaactggc aaagaacgat gggcagaatg aataggaaaa
    17401 tcattgtctt gttctttttc atactggtaa tacctcaaaa atgaaatgtc ataggcaaag
    17461 acagcaccca ttcaggtgcc gcctccattc agatcgaggg aatcctgaat aaatccatta
    17521 taaccgcatc gcttgacggc gtaccatgat ttattatagg attagtcgca cagggtatcc
    17581 aagtaagcgc agtagtcagc gccggttgcg tacttgcctt cgaacagctc gatcatggaa
    17641 ctcttgatga gggaccagtc gtccagcgtg ttcagagaac cacaccagct catcggggtg
    17701 gtggtattct tcagtgtctc aacagagcca gccaggacag actctttcag gcgggtgtca
    17761 gcggggatct gaccgccctt gtcaaccagc tcctgcccgc aggtgccggt aacaatggtc
    17821 ataatgaagt caaatgcagc ctgcgggttc tcacaggaag aggagatggc cagagagttt
    17881 gcaccggcgt aagcagaagc atcaacattg ccatcgactt caggatagtt gaagaatccc
    17941 cagtttacag gctcaccaac agcagcatca acctcggaag tgacatagtt tgcgcaagca
    18001 accatagcag ccgtgtcaag gccaaccttg gtctggctgg caggataagc atcgggcgcg
    18061 ccctcggcca gataaccagc gttcacgaaa tcaatgattt cctgtgcagc cgttacagca
    18121 gcttcattgt cagcccagcc accgttctta cccaactcgg caatggtgtc ctcgcccaga
    18181 tggcggacca gatggttgta gaagttgaag tttgcgtaag caccgtccag tgccagcggc
    18241 tcatagccgt tgtctttcag ggtctggcag actgtcagga actcattcca agtcttgggg
    18301 gtgtcggtga taccgcaggc atcgaaaatg tccttgttgt agaagatgcc gccaatctgg
    18361 ggctgctcta cgatggagtt gagataacca gcccactctt tggcctgatc attgaagcaa
    18421 gcgtagctga agtcatcata accgacagcg tctgccatct cggtcaggtc gtaggtgtac
    18481 ggggcataag cgtgaccgat acggttgtaa tcatcctcga aaatgtcaag cttttcgcca
    18541 gattccagtg cggcagaaat cagggtgttg acatcacggc ctttccactc gatgttgacc
    18601 tgtgcgccgg tttcagcctc gaaagcgtcg gcggcggcct gaattgcggc tgcctgaacc
    18661 tcaccggcag accacatgga ccacatggtc aactcaacgc cctcgtactt cttgccatcg
    18721 gtagccgtag actcggcagc cgtggattca gcaggagtag cagaggtagc ctccgagttt
    18781 tgagtattgc caccacaagc agccagagat gctgccatag cagaagccat caaaagtgca
    18841 acaatcttct tcattcgtgt tttcctccta agtgtaaatt ctgtgtcggg attacctccc
    18901 tttcgcggtg atttaagtgt agtgcttttc cagtttttta gcaatcatta tcttgctcac
    18961 ttgttttaca atcttgctat ttcgtctctt tttggcacat cgccggattt tcgcgggcat
    19021 ttattatgca cttttaccat ttactatttt tttgtaaaag ttggttgttg gcaaaaatgc
    19081 tgaataccgt cttgcgcttt tcagtcttgt gcaatatcac ctgacttccg tcgtttctct
    19141 caaaacctag tactcagctg tcaatcagca tggtgaactc aaagatattt tcaatctctt
    19201 tttctgtcag atacagtgca tcgagattgg ttaaaccgat tatttttttg attttttcat
    19261 gaacgctttg gttctgggaa atattgatat taagttccat cgcctttgtc tgcgaccctg
    19321 aagtcacgca taaaatttac aagatcatag gaactgatct cgtttcagac tgtagacaaa
    19381 gttccttcct gtgagggagc tttgtctaca gtctgaaagt aaccggaact ctccggtcac
    19441 tctgcattgc catctctgaa aaatatatcc agttttccca gacatttgat caatgtcttc
    19501 atttcttcct cgtccagatc ccgtatgatg gtgtggatca ttttcctgtg gaaatctctg
    19561 tggtggaaaa aagcttttct ccccttctcc gtcagggtta ccagcaccac ccgtttgtcc
    19621 tgtgtgctgc ggttacgctc caggtagcct tttttctcca gactgttcat attcgtggtc
    19681 agggtaccca cggtgacatg cagttccccg gcaatcctgg acatgctctt gggttcctcg
    19741 atgccgatag cctccagaat gtgcatgtca ttatttgtaa tatccttgaa ttcgtctgtt
    19801 atgaccgcct ttgcctctgc atccatcacg ttatggaaca ggttcaccag cagttcattc
    19861 agtacccggt tgttgctctt ttccatagtt ttcccaataa atgtctctct ggtataccgg
    19921 caatgctccc ggaccgcagg cttcctgtct accggaccgc gtccatgccg ttgtcgtgtc
    19981 tttagtcgtt atcagtatac cgtctaccag gtcatcacgc aagccccaat cgtaagcccg
    20041 gcaccgaatc ccgccagaac gatgcggtcg ccgcggtgca gctttctctg cctgttcagt
    20101 tcgtccagaa gcaccgggat ggaggcagag gacatatttc cgaccctttc cagattggtg
    20161 gggaatttgt ctctgtctgc atgcagtctt ttcgccactg catcaatgat acgtgcgttg
    20221 gcctgatgca gtacgaacat atcaatatcc ggcacagcaa gtccggcatc tgacagtgct
    20281 tcctcgatgc actgtggcac ccgtctggtg gcgaaccggt atacttcctg tccgtccatc
    20341 tgaatataat gttccctgcc atccgtcggc tggttttggt ccgtttttgc cgctgaagtc
    20401 cttgcatatg gggttgtcag ttctcttgct ccgcattgca ggactccgcc accggtgccg
    20461 tcggagtgca gtgcccggcc cagaatgcct gctgccggta tccgtttctc ctccgccgtc
    20521 tcctgcatcc ctttctctgt ctccggcaaa gcgttactgt attccaccgc ccgagactcc
    20581 gtcctgcatc tctccataac cacggccccg gctccgtctc cgaacagaat acagctgccc
    20641 cggtccgtcc agtccaccag cttggacagt acctcactgc cgatgatcag ggcatttttc
    20701 gccaatcctg tcctcagata agcatctgcg gtattcagtg caaataaaaa tccggagcag
    20761 gccgcattca catcgaaagc caatgcattc accgcaccga tggccgcctg tacctggcag
    20821 gcgcaacagg gcagatactg ctccggagaa cagcttgcta ccagaatcag atcgatttct
    20881 tctgcagttt ttcctgcctg ttccaatgct ttgtgggccg cttctgacgc aagtgtcaca
    20941 accgtctccc ccacagacac atgacgttca gcaataccgg ttctctcccg gatccattca
    21001 tccgatgtct ccaccagctg ctccaattct ttatttgtaa cgatccttcc cggaagagca
    21061 cttccggttc cggtgattct tatggtcctc acgccatttc ctccatgata tccactgttc
    21121 tttctatttt ctgcgccctc caggcattgc tgccacagaa cagcagtcca ttatccacat
    21181 ctcccattgc cgcattgatc aa
//
LOCUS       DAWEJW010000048        20529 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1018_length_20529_cov_52.573654, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000048 DAWEJW010000000
VERSION     DAWEJW010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 20529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20529
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1018_length_20529_cov_52.573654"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2144..2243
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15798..15897
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18240..18339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6058 a         3728 c         5411 g         5032 t
ORIGIN      
        1 atacattgct ggaagaggct tccggtgggg aatggaaacg ctatcggggc ggttccatct
       61 gtgccttgct ttcttttggt gaagatcatg tgaagaacag gatccgccat gtactggatt
      121 atcgcaagcc gccgttctgg gtcatgattg gcgcggcagc agtgatcgtg gtactggcgg
      181 tgtgtctgtg cagcaatccc ggtggaagtg ggaacggggc aggcacggcg gatcgtgcag
      241 atacagacga cagcgcagga gcagaaatgg atgtagacag caatgcggag acgagtggaa
      301 atattgcgca gtttccggac agcatcaaga caccggaaga ttttgcaaag gtaacgattc
      361 atagcagaga agattttgcc gctgtttttg atgtggaagc aatcgaagaa aagcattcct
      421 ttgcagcgga tcaagtggct tatgatctgc tggttcgtga aaacaatacg aatgccgcgt
      481 atgcgatatt caaagcactg gcggaggaac ctgcatccga gacttataga aaatatacag
      541 atccggtaac ggcagcggtg tcactgttgc acctgggtga tggaagcgga gccgtgaccg
      601 agacgctgta tccggcaggg cggcaatatc cttattatcg ggaggactct gaccggccgg
      661 gagaaggctc tgtggtgaat gtacattata cttttgcgga tggatctgct gtagatattc
      721 ccatggtcat ggcggaggag atgacgcagc tgtggctctt atcctttgga gatccgggca
      781 gtgcggagga ctcgggatat ggtccgctca ccggtgatat ctgtaccaga atggtctatg
      841 gggagtacga actccgacaa ccggacagag cggtctactg gggctggatg gactggtatc
      901 ctgtggatgc cggcagttat tcgacttatt atccccatgt acagatttca aattacggga
      961 tctataccat gagtgaactt tggggaacgt ccggcagtgt atttcgctgc attcaagcgg
     1021 aacgcatcat ggcaacgggc tggtatgacg ttacctgtga gcggggcgca tatgacacat
     1081 cggatgccta tacacagctg caaaaagatc tgccggatgt gacggaatgg agcggcggca
     1141 aggtctatta tctgacagat cccgatgagg cgcatcttat gacggatgag tggatgccga
     1201 gggtacttcg ggagtttgac atgagtaccc atactgtcac cgatacggtt ctgccggtga
     1261 atttgcagct caacagcggc acgacgcttc atgttcatga cggatattat gacatttaca
     1321 atagtaatcc atccataagt atgcggtttg cacttagcgg ggcggatagc cagcacatcg
     1381 aaagagcaga cattctggcc tatccgggtg tggtatttga tgtatcggag cggacagaaa
     1441 ctgagacatc ggcgcagctg gatctggatg gcgatggtgt gccggagaag atttctctgc
     1501 atcctgcgga gccggtaacg gggtattctc tggagcaata tacgatatat ctgaatctgg
     1561 gacttagtca aatgaattgc tatgacctgc aggatgcagt gttggagata gacggacaga
     1621 cggctgagat tccggacagc acgggaaata tgagcaattc gatcttcgct ttcagcccgg
     1681 acggagaaca gctcctgatc gcattgtatg atgacggcga aagcgcagat ccactgaccc
     1741 gaatctatca ctatgaagac ggaaaaccgg tggaaacaga ccggctcgca caggatctgc
     1801 gaaaggcatg gatacaggat ggacagatgg ttatcaccga accatatttt gcggtgcaga
     1861 atgattatat ccagaaaatc tatcaggtaa catcgaacgg aagacttcgg gaagtgccac
     1921 aggaggaata tgtattaagt gcctgggagg aagtggagct gcggcaggat ataacgctct
     1981 accgtacacc ggacggggat gagacctttg tgctgcccaa aggaagcaag gtacgcatga
     2041 cgaagctgga tgcaacacag gactggatct gcatcgagat tacggatggt gtgaaggggt
     2101 ggtttcggtt acgggatgga acggattaca gtgactattt cagnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnngtgtgaa ggggtggttt cggttacggg atggaacgga
     2281 ttacagtgac tatttcagtg gcttgtattt tgccgggtaa agtgtgaaaa ggagatacag
     2341 tcggtaatat gttccttatc tcaaaagatc taaaaaacgt attatgtgga gtaaaaatag
     2401 aagaggaaga gttcctgctg gtagtaccgg cggaggacaa agcaactgca gatggcatga
     2461 tcgacaatgc agcaaaacaa tcacaggcgt tttcctggtg gctgttggaa gtatacaagg
     2521 atatcataaa ggatactgtg aaatgagata aataatgaaa aatggggagg ttgtgcttat
     2581 gatgcagcag ttaagtttca gtccggtgga aaacatattg acagtcctgt tcgcagcggc
     2641 aattctggga tatcttctgt accttctggt gcggagctta cggaacaggc aacggggata
     2701 tcatacggtg aaccgtgcaa aagtctatag caaggcacag gctactgcgg tgaaccaacc
     2761 ggtatatctg gcaaacggca cagtaaattc cggagtaacc gaggcggaaa acgtcaagac
     2821 tgtcattttt gaaaatctgg aaaaagaggg cgaacagatc accttggaag tttccgacag
     2881 aatctacagc gaattgcagg aaggcgattg tggaagcctc agctgccggg acggaaaatt
     2941 agtgagcttc ggggcgttac agaacaggga tgacagagag cggagcttcc gcggataggg
     3001 aagcagagaa cataggaggg actttatggt ggaaggcatc tatcgtttgt ttgacagtat
     3061 atggcgtctc agtctggtgg gaagctattg tattttgttg gtgatactgg ccagattttt
     3121 actgaaaaaa gccccgaaat ggtgttccta tctgctgtgg ggaatcgtat ttgtgcggtt
     3181 atgttgtccg gtgctgccgg ggaccaggat cagtctgatc ccggagcgtc tgttgaccgt
     3241 agggacggcg acaacctctg cacagttgac tgccgggaac agtgccggat cagttgacaa
     3301 tgagggagtt tataatggca gccaccgtgg agacaatact gtggatgccg tgctgtccgg
     3361 ggcggataat acgggaaatc tgaatgccgg gactttagaa gccaccggaa atagggaagg
     3421 ttctgcacca acactgggac aggatggaaa cgtagaaggc atgaatgaaa ttggagatga
     3481 cggtgtcggt gaaattcagg caccgggtaa tgttacgaat gccttggatg gtggttaccc
     3541 ggtggataac cgggataatg cggacgattc tttgcaaagg atcggtgcaa caatgggaaa
     3601 aacatggttt cgtgtcctgt tgatcgcatg gttacttgga atgacaggat ttatggggta
     3661 tcatactttt tcctacctgc gtatgcgtca ccgtatccgc agacctgaca gcggtgtcag
     3721 acaggtggaa ccgggaatct gcgagattga cggaggacac ctgtccttcg tcatggggct
     3781 gatccatccg gtgatctatc tctcttcggg gctggatccg gagagcagaa aagtcgtgtt
     3841 gtgccacgag agagtgcatc tgcagcgcag ggattacctt ttcaaaccgg cagcgctgct
     3901 gatctgctgt gtgcattggt ttaacccgtt ggtgtggctg gcattttatc tgatgaatat
     3961 ggactgtgag atgtcctgcg atgaaaaagt tgtgaagctt ctcggcgagg agagcaagaa
     4021 gatctattcc tatacattgc tggaagaagc ttccggcgga gagtggaagc gttatcgggg
     4081 cggttccatc tgcgcactgc tttcttttgg cgaagaccat gtgaagaatc gtattcgtca
     4141 tgtactggat tatcgcaagc cgccgttctg ggtcatgatc ggcgcggcag cagtgatcgt
     4201 ggtactggtg gtgtgcctgt gcagcaaccc cggaggaaat gggaacgggg cagatacagc
     4261 aaatgtgtct gacgtttccg atgcgacaga agaagtgacg accggagaat tcacagagga
     4321 cggttcagac gatgaaggca ggaaaaccga atggctggcg gcattggaac aggggattca
     4381 tagcagagaa gaattcagtg cgttgtttga tgtggatacc ttggaggata cccggtcttt
     4441 tgccggacag gacggcattc aatatctgga atacaacggc gttgcggcga gaaaactgca
     4501 cagacagatc ctggatgggg ataccgcagc ccacgagact tataaggatc cggtaaaagc
     4561 ggcggaagca ctgctgcatc tggagggtgg cagcggagaa atgacggaac tgctctatga
     4621 agcggaaagg cagtattttt ttttccaaaa agattccaca agacccggcg aaggctctgt
     4681 ggcaaacgta cattatattt tcgctgacgg atccgatatt gatattccca tggtattcgc
     4741 cgaggagagc agtcagatct ggatgctgtc actgggagat atcgacagta aaggacggca
     4801 ttcttacggt ccgttggatg gagagaatca ggcaagaatc gtctatgcgg aatatggacc
     4861 gcaggatgtg gaagaggatc ctttggcggg agcccatccc ggggattata cgcaggtacc
     4921 tcagggatct tttagagatc aatatgctac ttatcaggtg tccagttacg gcatctatgt
     4981 ggcaaccggg agtgatgccg gactggattg tgtcgtaccg gggtatattg cgccgggata
     5041 tgcagcatcc cactggtcat gcgtatatca ggatcggaga ctcttcaata cttcggatca
     5101 gtgtgactat ttagattgtt accgggatga gatagagagc gtgcgcttgt actacctggc
     5161 atcctctgac tatcaggatg gcgacttgga catctggatc gacacgatcc gggaatatga
     5221 tacttcggga cagaccggag aaaacggggt cacggactgg aagctgccgt cggaggcgca
     5281 ggatttcgac aaggcttcgt ttcgtgcaga ggacggttac tggatcttcg aaaatagtga
     5341 tggcagcgaa cagctgaagc tgccgatcct gtctgtaagg ccggtatgga acgggaaaac
     5401 agcagcggaa ctgaccgcag aggagacaga tgcctatgca gccgcacaga ggcaggaaat
     5461 cctgacaaaa gtaggagttg tctatgatgt gtccgagcgt gcggcacagg agacctatgc
     5521 gctgcttgat ttggacggag acggaaccgc agaccggatc acgcttcgtt ccaggatggc
     5581 gcaggcactg aatacgccgg atcattctcc gctggatcag tatgttttta cggtcaacac
     5641 gacgagtggc gagatgcgga cggcacagaa tctggggaac agcatctatg ccttcagccc
     5701 ggacggcaga cagatattgt tagcccttac cagaaaagat gagcaggggc agtacgagag
     5761 ctttctgttc cggtaccgga acggcgaatt acaggaagtg ggaagcttcg cacaggatat
     5821 tcgtaaaatc tgggtgcaaa acggacagat cactaccggc cggaacagtg atgatatcat
     5881 atttgcagaa cccaatatga ccggtgcaaa cacaattcta gattccagaa attcagatgg
     5941 aacaccatca ggggcatatg tagaaacatt gcaggagaaa atttcggcag atatggttgc
     6001 cgggaaatta ccatttgtag ttacctccgt cattagggaa aatccactga gattggaggt
     6061 ggaactgacc gagatgacag acgagaatat aaagatcatt cgatcctatg aaacaaatgg
     6121 cagtgccata acatttgcac aaagcagtgg caatgtacaa gagctgcagc ataataatga
     6181 ttcgcaaagt caaatagcac cccagatttc aaaagaagat gaaaacgatt gattaacaga
     6241 aagagttgac agagaagaat aatgatgttc aggcggtaat tatcagcgga aatcgaatag
     6301 atcatcgttg aattaccgcc tcaaatttta tgcaaaggta tctggacggt aaatagaaat
     6361 aagctacatc ggaattgttt attttaatat gaaattattg gaaacagtta atgaaagtgc
     6421 aacaaaaata tgatatttta aattcgtaat ataaaaagag ttacggattt aaagtaagaa
     6481 atgcgtgaga attttaagca gtaagggaga ttatcatgaa aaaacaatac agattagcaa
     6541 catttgcact gtccgcctgc ctgttaatgg catgtggcgc ccgggagacc aatgcaccgg
     6601 agaacaacga agcggagaaa caaaccgaaa atatttccaa tatagacact aaaatagatg
     6661 aggcagagac ctccggagct ctcacagagt ccgaatcctg ggaaacaccc attgcaccga
     6721 aagagatcct catagatttc actggaatct cggaatttca cccggaagga actgcggcgc
     6781 agccctattc cctggaaatc ctttccgaga cgtataacaa tattactttt gcagacgaat
     6841 ggtacaatgc atcggaagac atttcccttc ccatgatcgg agaaaactgg gactgcttct
     6901 atgatgaaaa ttatacctat cagtgggagt atacctatca gtgggaggat ggaaaactca
     6961 gcattttcga tggtgagaac tgcctgtatg tactggaata tccgacagac aaatggtacg
     7021 tcaacggtaa taatgcctgc cttgagaacg gaatttttta cggggcttca gtaatgaacg
     7081 gttatgcgca gccggacagc tgctttatgt ttgcctatga tctggaaaat gagaaactgc
     7141 tgtggcgaag tgcagaccag acttataaca gcatgaattt tctcgtgaaa ggggacgtga
     7201 tcttctgcgg ctatggcttt accgcagagg acgattatct ctatcagctg gataaaaata
     7261 ccggagaggt gatcgaccgc ctgtctctga aaaagatgcc ggacctcatg gcagagaagg
     7321 atgaccggtt gtatgtgcac acctatagtt atgattatgt gattgggata ttttaaattg
     7381 aaaatatcaa aataattgat atcggtcgac tatcttatca ttaaatattg aatgtgcaat
     7441 aaaaatatgc tattttaaaa atgtaacata aaaaaagtta catttttaaa aatggtgact
     7501 atatgaagaa ttttttggaa aaaacattgc gtcaaaatgt gatgatagaa gaaactgaat
     7561 atttgaatga gaagctgccc ctggcctttc gtggaagata cactttttat aaggttgaaa
     7621 caaacggttc accatggata gcaattcaac ccaaggcaga tgttggcttg gcagcgctta
     7681 gaaaggactg gattaaaatt gaaaaggcag caggtcttaa ttgcgccatt tttttggatt
     7741 ctacatcatt ttatattaaa gaaaaattat tggaagaggg aattcccttt gtactgaaag
     7801 ataaacaggt gtacttgcct tttatcggat atttgctgtc caatgagaat gaaaggaaaa
     7861 tttctccggt tcatttaatt tcatttttaa cacaaaaagt tatattggtt gcaatatatg
     7921 aaaaatggga aaatgttact gcatcaaaag ctgcaataag actaggcgtt acgaaaatgt
     7981 ctgcaaacag gtgctttgat gaaattgagt atctgaatgt ggatattttg ggtatgaagg
     8041 gtaaatccag ggtgataact gttccggcgg atattcagaa actgtggaat gatgtacggg
     8101 tcatattgag aaaccctgtg attgcaaggt atgagctaaa agaggatatt caattggaaa
     8161 aaaaggcagg aatcactgca ctatgtgagt actcattact ttcggataat gaatatccga
     8221 cttatgcaat taccaaaaaa gaaataacag aatccggaat tagaaaaatg aagcaggtac
     8281 attccgggga agaaatagga tgcgttgttc tggaactggg atactttatt gattttaaga
     8341 ataagaaact ggaagatcca ttgagtgtga cattgtcgct tacagagagt gagaaacagg
     8401 atgaaagaat tggtatttct ataaagagga tgctggagga atacgtatgg taaaaggctt
     8461 ttgaaaaatc ttgtgggagg aggattcaga aaaagtatgt acaaagcaaa gatgaggtgt
     8521 gtggcattat taataattat gttaagcatg gtaagctgtg ggcagaatag accggaacag
     8581 aatgatacag aacagaatac ttcagaagtt accgaggaga tgacacctaa taccataacg
     8641 actacagcag tatcaaatga agagactgaa ccggttgatt tatcggaaga tttcagtttg
     8701 gaaaatttaa aagggaaaac cgatccggaa gtaatcagac aggaattatc cgtccttccg
     8761 ttaaatatag aagaactcaa agattatccg gtgtatgtct tggattggca gtcaagggaa
     8821 tgtagcggag cagaattgct ggacgaattt tacgagcaga catcagagaa tcaaccggcg
     8881 caattagtga ttgtacacta tttagtgagt gaggaattga ttacagcgag atgtatttcc
     8941 attccctgaa cataattaga aagcagccgg aacaaagtga aattgaacta ataatgatag
     9001 tatttacgga tcaggaagat gtctccgatg aaaaggcgga acagtatgtg gaactgctat
     9061 atgacgggaa gagaccggag aacgtagagg tgcgtccttt cctgaattta cggaagacaa
     9121 gttaagttat acagtgggaa gttgtggcag cagtgcttgt ttttttacct gttggaaaaa
     9181 atgatattgc gacaacgtgc gagggggatt acgaatgaaa acttttaaaa caatagcatc
     9241 attgctggta ggtacagtat catctttgct gttaatggga tgcggagaaa tagacgccgg
     9301 gacgaaaaca tctgcgtggg agaaagagac tgctacaatt gtgccggaag aacctaaaac
     9361 ggagattata cttggagata tcgggagagc gtcgacatta caggaagcag tagctgattt
     9421 taatagtaaa aatgacggaa taacaatcac aatacatgat tattacgaag aaaatataag
     9481 cgacggaatt agtcgtttgt atgatgatat tttgacagga aaaggacctg atatcattag
     9541 tttttcgaca gatgaaatgg acgtggaagt gcttagagaa aagggtgcga tagagaatct
     9601 tattccttat ctggagaaaa gtgatgtgat tggggaagag gacatcgttg attccgcgca
     9661 tcaggttttg acatcggatg gcggcttgta tatgttgcca acgaattttg tgttatatac
     9721 attgataaca aaagaaaaat ggtgcagtaa caaagaaaat tttaccttcg aggaagcctt
     9781 gcgggccgtt cgggaatgta aagaggatcc tatgatttca cgggatttat ttttacagga
     9841 aggggcaatc tgcggaggat attccgggga aacggaagat aacagattgg aacaatatat
     9901 aaaaattgca gaacaattac cgcaacaggc gatgtttcaa acgaatgacc ttttgatgcg
     9961 ggaaggaaaa attccattca atcttgagtg cataggtgac atgcaacaat atttatataa
    10021 aaaatccgta tggggtgagg atgcagtcta tacaggattt ccgggagcag aaggaaacgg
    10081 aagaatattt atttttgtca actgtttcgc cattaacagt caaagcacgc acaaagaaga
    10141 agcatggaag tttgtggaaa gctattttac tgaggaagga cagaaaacga ttgctcccaa
    10201 ttggaatttc tcaatattgc aagatgtgct ggatcaacag cttagtgata gcagaaagca
    10261 ggaatattat cagacatcgg atggaaaaag agagaaactt cctattctga catacgaaat
    10321 tggcggtacc tatgaaaatg tttatgcggc acaagatgag gatatacagg atgtaaggga
    10381 gatgataaat aatacgaagg taatgcagcg cagtgacctt cctttaatag ggattacgca
    10441 ggcagaggct ttgtattatt ttaacggaga aaaaagtata gaagaaacag cagcggctat
    10501 aaggaacaaa attgatgaaa tgccgaatgc aacaaatgca cagacagata tggatgtcgt
    10561 tatcaatata ggggattttt cagtttcctt atatgaaggt gagcaggaaa tattggggaa
    10621 gctggacaag atggggctgt tgtatgaaaa ggttgaggat tcggataaca aaaaatataa
    10681 ttattattat aacgtaggtg atggggaagc tcaatttata caagtttatt ttcttgaaaa
    10741 agaatgtgtc agaataagaa tcagtagtaa tgaaacattt gcacatacct cgagaggcat
    10801 atattccgga aatacttatt ctcaaatggt cgaacaatat ggagattctt atgaaaaaca
    10861 tacttatata ggcaaagaga gatataccat ttatcgttat gctttaggag aatgtttcca
    10921 tgaattcggc ataccggggg aaacgaccgg tgaaatctat aatgttgatg tttatgtttc
    10981 cggtcagatg cctatctatg attatggaga agagattgta gaggattaga aattttttga
    11041 aaatatgtaa ggcgatgaga cggactatag ggaatggctg aatgcaaacg aaggtaatgt
    11101 tgaagaactg gagattccat atttttcatt tacagaagaa aatagaagat aataggggat
    11161 aaacaaaaag atgacaaaaa agcagaaagt agtatttgcg gttgtggcgg tagtgattgt
    11221 gctggggatg attatgtctt acgcattttt gatgaaaaat cagcatatag acagtacacc
    11281 tgaagaaaaa aagatgcctg ccatgagtga tacagaagac tatgaagtac gtgaaagtga
    11341 tgaaagcatt caaagcagcg aaacagaaga agattattat atgacatcta caggagtaac
    11401 agcagaggag caatttgctg aagagattaa aaatgatgtt ttaaagcatg attgggatgc
    11461 gctatccgag aaaatttcat atcctattgc aataaatggt actacggtta ataatcgtga
    11521 agatttttta aaactcgata ttgatggaaa gctgaatcaa gaatttgtag aggctattcg
    11581 tgctgaaaca tgccgaaaaa tgttttgtaa ttggcaaggg gtgatgatgg gggatggaca
    11641 aatctggttt gacaatgttt ataaaggaac aggaccttgg aaactatcaa ttatcggaat
    11701 taataatatg ctcgagacag aatcggaaga tctgaatgcg gaagaaacga tagagccaga
    11761 tccggaaata gcagatggca gtgtggacga attatccatg gatgagatca aggaattgct
    11821 ttttggtgag tggaaagtta caaaattgct tggctttaca cacgtacaaa acgattatac
    11881 gaattatccg gaaggacatg atatcatagg caatcatatt gtgataaatg aagatgtatt
    11941 ttccagtgaa gggcttgaaa aatatgaacg ttatcaatgc gagattttag aaccttctta
    12001 tgaggtatct cctataaaag aacgtaattt aatctattat gtaaaggaag ctataaaaga
    12061 cgatccggaa ctctatgatt ggattctgaa ttatgatgaa tttcagaatc tggaaataag
    12121 aggcacgaga gatggaataa aacgacagcc tccggcagtg gacataatga tttccggtaa
    12181 tacacagttt gttattctta cgatggattg tgctttttac ctgttggaaa aagaatgata
    12241 ttgcgatgat aattggggga ggaacaaaaa atgaccaaaa agcagaaagt agtatttgca
    12301 gttgtggcag cagtgcttgc agttgtggca gcagtgattg tgctggggac agttttgtct
    12361 tacatatgct atcattttat ttatgggaca agaatcactt cccgggaggg agaagcatac
    12421 cacaaattag agggaacggg agtatacagt ccgttagcgg tgttcccttc cgcagatatg
    12481 gataccgttt cacaagattt ttattatcag acccgtgatg aaatctttgc agcaacctgc
    12541 cagatctatt tggaaaatca atataccagg gaacagtatg aggcagaaac agaacgtctg
    12601 cgtaatttgg aattttctta tcaggatcag acgaatatgc tctatcagga tgaaaaaaat
    12661 tattgcagcg ttgcatatgt ggcaatggca aattggaccg accgatatga atatgccatc
    12721 acgttagacg acagtaatac cattatttat gtctatttac agaacatgga tgcaaaggac
    12781 atacatatgc aggctgatta ccttccgaaa tattttcagg ataataatgc cggaaagcat
    12841 caggatacag accctatgac cgcggattac agaagtttct atgcgttccg gataggagat
    12901 cattatatag actgtatgga cttggcagat cagattgaaa tcgccgatac cgagccgaag
    12961 attcaggcag aggatgtagc acaggaagtt gagacaccgg aacctcagac gattgcttcg
    13021 gatacatctg aagaaaagga cacgagcagc atgctcatta attctgagat tgaaataagt
    13081 gcagatttag atggagatgg gaaagaagat atagtgcgtg ttgtagataa cggcgatatc
    13141 gatgacttag caaaagatgg tacacgactc atagcgaatg tgaatggcgc agatacggca
    13201 ataaaggatt acgaaggata cgtttatagt tcgaggatta ctaccggtga tctgtcagga
    13261 gatggtaaag cggatgtatt attggaaagg tacatcttcg gaagcaatta tggtgctgtt
    13321 acaatttcga ttctgcattt agaggatacc ggatgggtgg aatatccata taactttata
    13381 cataatccca atattgattc agaacagcct gataaattcg gtgatgttaa gattgatgac
    13441 aagcggccaa tagacagtta tatcggggca accatatttg aaaaagacgg aaaaacgatg
    13501 gttcgtttta tctcattaca atatgaagtc gatgacgaaa gcacggtaaa atgtacagag
    13561 gcatcctatc gggaagatgg gtggtatata gaagatatta gattaattga taattattat
    13621 gataagagag aagaactatt agcgccgcag gatgatacac tgacgataag aggagaagtg
    13681 gctgggaacg aggatagaaa aatagtatac gagatagcaa aagaattttc ggaggcctat
    13741 tttcaaggtg attcggaaac aattaaaaag tatttagtgg aagattacag tggaacactg
    13801 gatacttatg cagagttccg ggaatctaaa ggaaaagaaa ctgtttgtat aaataggata
    13861 aaaggattag cggatgttgg ggatgaaacg ggggtaacct atacagtgca ggcggaattt
    13921 ttgccggcag gagaagactc cttgttttat ctttttatgg actttgagaa gcaagaaggc
    13981 ggatggcgga taaggacata cggattagaa aaataagagc ttaaggaaag cacacaaaaa
    14041 tttaaacttt tcaatatgca gttgacacag tcagtgaatt gccatattct aattatagag
    14101 aggtctccat taacctttgg taatcaagcc ataaagagat gaacattaac gagtgggaag
    14161 attcgtcttc ccacattttt taatccagta ttatgtgtgc actaggggaa aaactaataa
    14221 aaatgcgtac tcaccggttc agccaccaca cccccagatt cagatacccc gcgaacgcca
    14281 cccataccag ataaggcagc atcagccatg cggcaggctt cgagatgcgg taaaaggcaa
    14341 cggtagtggc gatgatcagg atccacagag cgaccagcca caaaaaggcg aaaagatacc
    14401 atcccaggtt gaagaaccag atggaccaga aaaaattaaa gataagctgt acagcataca
    14461 gggtgagggc tttcgtgtcc tcaccttttt gcatgacaac gagataagag gcgattccca
    14521 tgaggacgaa caggatactc cacaccaccg ggaagagcca cataggcggt gtcaggggag
    14581 gctgattcac tgtttcaaag gctttcatgc cgtccatggt gatccaggcg gagagtccac
    14641 ctgccgccag agggatggca acggcagtga tgaggttttt ccagtgaata ttgtgaaatt
    14701 tgtcggagaa ttgcatggga gcctcctttg aaaatacgat tttgcatgtg tcaatatccg
    14761 tgtatttctg attagtattg tatgaaaatg agataaaaat tatgtgtcta gctataaaat
    14821 gaggacaaaa atttgggact atcggcaaac ggaggttttg gtgtatgatt aagagtataa
    14881 taaagcatta tgactatcag aaacaggaaa tatccaagaa aaggtaatag gtaacatagc
    14941 tatgagcaca gaacaaacac agggttccct gacaaaagaa gcaatccggc tggcgtggcc
    15001 ggcggtctgt gaatcctttt ttatcgcact ggcgggtatg gtggatactt ttatggtgag
    15061 tacgctcggg gccaatgcgg tggcagcagt agggctgact acacagccga agttcctggg
    15121 gctggcactg ttcatcgcag cgaatgtagc aatctccgcc ctggtggcga gacgatttgg
    15181 agagaagagg cagcaggcgg ccaacgagat tctggtgatg acgatctgct tttgcattgt
    15241 ggcagcggtg atcatcagtg cactgacggt gggattcgcg gatcagatta ttgaattctg
    15301 cggatcgagt gaggacacgc atgccatggc ggcgagatac taccggatca tcatgggtgg
    15361 catgatcttc aacgtattgt ccatgggaat caatgcggca cagcgcggcg cgggcaatac
    15421 gatgatcgcc cttaggacga atctgatctc caatctggtg aatatctgcg gcaattacct
    15481 gctgatcaac ggccatttcg gattcccggc actggggatc accggagccg ccattgccac
    15541 ggtattcggt acggtagtgg catgcgtgat gagcatcgtc tcaatctgtc cgaaagacgg
    15601 atttatcagt attccgtaca tgatcgagca tcacatcaga ctgcgattag aaccgctgtt
    15661 ggagattatc aaagtcggct attccgtgtt tattgagcag gtattgatgc gtatcggttt
    15721 tatgtctacc gctatgatgg ctgcgaaaat gggtacggaa gccatggcgg cacatcaggt
    15781 aggcatgaat atcctcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
    15901 aaaatgggta cggaagccat ggcggcacat caggtaggca tgaatatcct cggtctgacg
    15961 ttttcattcg gtgacggtat gcaggtagcg gcggtggcgt tgatcggccg gagtctgggc
    16021 gaacgtgacc cggagaaagc gaaaagctat ggagctatct gtagacgtat cggtatggga
    16081 atctctgttg cactggcggt tatctatttc ttcggagggg agaccattta ccggatgttc
    16141 tttagagaag aaaatattat tacgtacggt gtgaatatta ttcattgcat ttgtatcatt
    16201 gtcctgttcc aggtgtctca ggtcatctac atgggatgcc tgcgcggcgc gggagatacc
    16261 gcatacacgg cagtagcttc tacgatcagc gttactttga tccgtacggc ggcatcctat
    16321 attttcgggt ttactctggg actgggaatg accggtatct ggatgggtat tctggcggat
    16381 caggtatcca gattcctgtt tgcgtcgatt cggttccgac agggaaaatg ggtgacaatt
    16441 aagatttaat gagcgcaggt aatgagctta tgataataac aaaactcccg gagcaatatc
    16501 gcaccgggag ttcttgcttt caaataacat gcaatgattc actgtgagtt ctatttcgtc
    16561 ttcaatgtct ttttcatagc agtgaaagtc tctgcactga tcacatgctc gatccggcag
    16621 gcatcctcag cagcaacggt gggatccaca ccgatggaag tcagccagtc cgtaagaaat
    16681 gtgtgacgtt catagatttt ctctgcaatt tcccgcccct tatccagcag ggtgatgtag
    16741 ccttccttgt ccatttcaat atattcccca ttgcggagat tcttcatagc tacgctgacg
    16801 ctgggcttgg acacacccag atcgttggca atatcaatgg aacggaccac accgttcttt
    16861 ttcttcagca ctaagatcgt ttccagataa ttctcagcag attcctgtat tttcatgtaa
    16921 tgaccatacc cttcccatac atagcatttt gaaccgaaat acctgctcta catgtggaaa
    16981 cttaaccatc cgcaggcatt acaataagaa tagcagaaaa gggtaggaaa tgcaagattt
    17041 caacttaatg agaaaaaata tcaataagaa attcctacga gatttctatg aaaaaaatcg
    17101 aaaaaatata ttgacagtaa aaaagggtta gtatatacta accacgtgaa ttgagaatga
    17161 atctcattaa gagctaatga gaaaccacaa gcagttcaat actattgaag ggaaggaagt
    17221 gtgtatgatg cccctgagta tggtaggtgc cggtgaagag aacacgatcc agaggatcgg
    17281 cggcaaagag gagacgagga agtttttaga gaccctcgga tttgtagtag gtggtaaagt
    17341 gacagttgtg tcccagaccg aaggcaacct gattgtgaat atcaaggaat caagagtagc
    17401 catcggcaag gatatggcca ataaaatcat ggtgtagcgg gcagctgtac caaaagatgc
    17461 taataggagg agaaaatgaa gacattgaag gaagttccct gcggacagac cgtaaaggtg
    17521 actaaactga atggtgaagg tcctgtcaag agacgtatca tggacatggg cattaccaaa
    17581 ggtgttgaga tctatgtgag aaaggttgct cctttgggag atcccgtgga agtaactgtc
    17641 agaggctatg agctgtctct tcgtaaggca gacgctgaga tgatttgcgt tgagtaaatg
    17701 atcttttttt tagcaggaag gttagctgaa actaattagt gaaagctaat cacatgagca
    17761 aaaaggaagc tctgaaagag cgttttgcga atggggtctg aatagttatt aaatgatttt
    17821 agaggaaaga ggtagaaaaa tggcaattaa gattgcactt gcaggtaacc ctaactgcgg
    17881 taaaacgaca cttttcaatg ctctgaccgg ttccaatcag ttcgtaggta actggcccgg
    17941 cgtaaccgtt gagaaaaagg aaggtaaatt aaagaaacag tacggcggta acggtgatga
    18001 cgtgatcatc atggacttac ccggtattta ttccctgtct ccgtatactc tggaggaagt
    18061 ggtagcacgt aactacctga tcggtgagag acccgatgcg atcctgaacc tggtagacgg
    18121 taccaacatc gagcgtaact tatatctgag cacacagctg atggaactcg gtattcccgt
    18181 tgtcatggct gtcaacatga tcgatatcgt gaacaagaac ggcgacaaga tcaacgtagn
    18241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtacctgggc taccgactgg
    18361 gcgaacgatg gcgtattcgg tgatggctgg catctgttca ccatcggaac cggtgcttat
    18421 gaagaagcag ccgagccgta tgatgatgca atgaatgtga tcaacgcatt cgtagaagct
    18481 gacggtgatg aagctctggc agcagtcatc gacagcgagt ctgaggacta tgatcccgca
    18541 gcagcagtag cagctgttca ggagttcgcg gcaggcatcg atgcatccgc taccgcagag
    18601 tatactctgg aagatgaaga gactctggca accgaggatg ttacttacac cggtgctgag
    18661 ctggcagagg cagttgatgt atacgcagca gacggcgcag aagctcccga tcccgcagac
    18721 tacggtatct gggtacccgg cgtacccgca ctgttagaga gcggtctgga tgcgatcgga
    18781 tgtgccgact ggctgaaagg tctgatcctt gacggtattg tagccggtgt cggtgcagtg
    18841 cttggtttcg taccccagat gctggtactg tttatcttcc tggcattcct ggagtcctgt
    18901 ggttacatgg cacgtatcgc cttcatcatg gaccgtatct tccgtaagtt cggtctgtcc
    18961 ggtaaatcct tcattcctat gctgatcggt tccggatgtg gtgtccccgg tatcatggct
    19021 tcccgtacca tcgagaacga cagagaccgt aagatgacga ttatgactac cacgtttatt
    19081 ccttgtggtg ctaagcttcc tttcatcgcc atggtggcag gtgctatctt cggcggcgca
    19141 gcatgggttg ctccttccgc atacttcctg ggaattgcag caatcatttg ttccggtatt
    19201 atcctgaaga agaccaagat cttcgagggc gatcccgctc ctttcgttat ggagcttcct
    19261 gcttaccact ggcctaccgt tggtacggta ctgcgcagca tgtgggagcg tggctggtcc
    19321 ttcatcaaga aggccggtac catcatcctg ctttccacca tcgttgtatg gtttactact
    19381 tacttcggat tcaccgagga aggcttccgt atgctggctg aggatgagat cgatatgagt
    19441 atcctgggca agatcggtca gtgtctggca tggatcttca tccctcaggg cttcggtaac
    19501 tggcaggcaa ccgttgcatc catcaccggt ctggtagcaa aggagaacat cgtaggtact
    19561 atgggtatcc tctacagcgc aggcgagggt tccgtatatg ctaacatggc agctaccttc
    19621 actgtagcaa gcggctatgc attcctggca ttcaacctgc tgtgcgcacc ttgcttcgca
    19681 gctatgggtg cgatcaagcg tgagatgaac aataccagat ggttctggat cgctatcgga
    19741 tatcagtgcg gtctggcata tgtggtatcc ctgtgtgtat atcagatcgg tacgctgatc
    19801 accaccggtg ctttcggtat cggcactgta gtagcattcc ttctgatcat cggcttcatt
    19861 tatctgctct tccgtcctta caaggagagc accactctga aggttgacac cgagaaggct
    19921 gcataagtaa aataaatgat agaactttcc cacaggtgtc cggcgtgact gcttgcaggc
    19981 aaagccagat acctgtgagg gaagtaaaca gatagaaacg taacattact tgcggatctt
    20041 cgataagagg atccgcagga tttcaaaata agagtaagga ggcagtctat gggaaccgta
    20101 gttgtattgg cgatactggc cggtgtagtc gcattgattg tccgcaaaat gataaaagac
    20161 aaaaagaatg gaaaatccat tcagtgtggc ggtgactgca gccactgtgg aggtcattgt
    20221 ggacactaag gaacaggtaa gctatcagac cacgaaaatg cagagagaga tcatattgac
    20281 caagctgaaa gaaaaaggct gccgtattac gaagcagcga cttcggctga ttgatattat
    20341 cctccagaat gaatgctcca gctgtaagga aatcttctac cgggcattgg aagaggatga
    20401 caagctggga gttgcaaccg tataccgcat ggtcaatctc ctggaggaga tcggtgccat
    20461 cagccgcaag aacatgtata aggtagctta ctctgaaaat tgtatgatgg aaaatgcctg
    20521 taccattga
//
LOCUS       DAWEJW010000049        20397 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1026_length_20397_cov_71.688133, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000049 DAWEJW010000000
VERSION     DAWEJW010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 20397)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20397)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20397
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1026_length_20397_cov_71.688133"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    18514..18613
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4708 a         5492 c         4537 g         5560 t
ORIGIN      
        1 gcaccgatct tgacattcag atgagggtaa ccgatagagt tacgtacctg ttcaaaagca
       61 cgtcctgctg cgaacattgc aaaagagctt acgaagggaa tcttacctac cagagacagt
      121 cctgcagcca cacccatcat gttggactct gcgataccgc agtcgatgtg gcggtcggga
      181 tatgccttac ggaagattcc ggtcttggta gctgctgcca ggtcggcatc caatacgacc
      241 agattgggga attcttctgc cagctccttc agggagttac catagctttc tctggttgcg
      301 atttttttca cggtattctc tgccattact gctcacctgc tttctctaat tctttttcga
      361 tcttatcaag gtctgccatt gccactgcat actcctcatc gttgggagcc ttgccatgcc
      421 agtccacact gttctccatg aaggaaacgc ccttaccctt caggctatgg aatacgatac
      481 aggtaggcat gcccttggtc tctcttgctt ccttgaatgc tgcacggatc gcatcaaaat
      541 catgagcatc tgcattgatc acatggaaat tgaatgcctc gaacttctta tcgataggat
      601 aaggagagca tacctggtcg ataggtccat cgatctgcag attattgtta tctaccataa
      661 cacagaggtt gtccagctta cggaagcctg cgaacatggc tgcctcccat acctggcctt
      721 cctcgatctc accgtcaccc agcagtgtgt atacacggaa atcacgattc tccatctttg
      781 cacccagtgc catacctact gctgcggaaa tgccctgtcc cagggatccg gaagacatat
      841 ccacgccggg gatatggatg cagggatgac cctgtagata ggaacccagg tgacgcagag
      901 taggcagatc ctctaccggg aaatatcctc tgtaagccag tgcggaatag agtccgggag
      961 ctgtatgacc cttggacagt acgaatctgt cacgctcctc catatcggga ttctgtgggt
     1021 caatgttcat ctcctcaaaa tataaaaatg taaaaatatc tgcggctgac agggatccac
     1081 cgggatgtcc cgctttcgca ccatgaactg cggtcacaat gcccttgcgg acatcattgg
     1141 cacgcttttg cagttctaaa ttcttcattg tctcctccat tccgccatat tggcttgtct
     1201 gttgcgattt acgttgttat actagcatac ttacgaatgc tagtctagta aaagaattgc
     1261 tcttctaccg taaaatattg ttgtcttcta tcaaacagct ttgccctgtc attcataaat
     1321 tttctcactt cgttcgaaaa ttcatttcat ggaggggcgc tttgctaata gaatttgtca
     1381 tatagtgcta gtacgcaagc gtccacgcac tataagtcaa attctatttc aaagttgttt
     1441 ggccatagta tgcattcttt ccatgcttgc gattatagtg cttatcctgc agctcctgcg
     1501 gtgccggctg aattctgggg ttgatcgtct ctgcacggta tgccattttg gccacttctt
     1561 ccaatactac ggcattgtgg acagcctctt tggcatcctt gccccaggca aacggtccat
     1621 gattcttgca cagcacagcg ggaactgcct tgggatcctt gcccagacgc ttgaactcat
     1681 tgacgatcag atgtccggta ttttcctcgt aagcatcctc gatctcttcc ttcgtcaggc
     1741 agcgcacgca gggaaccgga ccgtagatat aatctgcatg ggtggttccg tagcagggaa
     1801 tgtctcttcc cgcctgtgcc caactggtag cataggaaga atgggtatgc acgataccgc
     1861 cgatctccgg gaacgccttg tacagctcca gatgagttgc agtgtcggaa gagggattca
     1921 gatccccttc caccttattg ccgttcagat ccatgactac catcatctcc ggtgtcagct
     1981 tgtcataatc cacaccactg ggtttgatca cgaacagtcc actctctctg tcaatggcgc
     2041 tgacatttcc ccaggtgaaa gtcaccagac cataccgggg cagatccata tttgcttcat
     2101 atactttctg ttttaattcc tctaacatat acttaatctc attcctttcc acagcctgcc
     2161 aactttccga cacataggta tcgtgtactt ccgtacactc tgcaggctca aattgcatct
     2221 gaaaaattat tttgccgcaa cggctgcctt ttctgcggga agcagtttct tgtactcttc
     2281 caggaaagca tcaaatccct tcacatcctc aggatccggg ttcagtgtgg ttccggtctg
     2341 tcctgcgaag atcttgtcat tcagatagga agaaagagtc tcgccgtctt ttttctcctt
     2401 catgaaagct gccagaatag caatacccca ggcaccgcct tcactggctg tatccataac
     2461 ggtgatcggt gcgttcatgc ctgctgccat gacacgctgt cccaccacgg gagtcttgaa
     2521 gaagccgcca tggcccatca gggaatccac ggccacatgc tcttctttta acagaatgtc
     2581 catgccaatc ttcagggtag caactgcgct gtacagatgg gaacgcatga agtttgccag
     2641 tgagaaatcc gcatcgggag ttcttaccac catgggacgc cctgcatcca gtccggtgat
     2701 gggctctcct gagaaataat tgtatgctgt cacgccgccg cagtccgcag caccttccag
     2761 tgccttgcgg tacagcacgc cgtacagttc attcttatct acttttacgc caaagctctc
     2821 tgcacattcg cccagaagat ttacccaggc attcagatcg gaggtacagt tgttgcaatg
     2881 taccatagct accggcatac cgtcgggagt ggtgaccata tcgatctctt cgtgtacttt
     2941 ttccatagcc ttctccagta ccaccatagc gaaaatggag gttccggcag agacattacc
     3001 ggtgcggact gccacactgt tggtggctac catgccggtt cccgcatcac cttcgggagg
     3061 gcacatgggt acgccgggct gtaaggttcc ggtggaatcc agtaattttg caccttccgc
     3121 ggtcagaacc ccggcaggct ctccggctac cagtaccttc ggcagaatgt cacggatctt
     3181 ccagtcaaaa ttgtgtaagc catcgaactg atccagcatc ttctgatcaa aatccataat
     3241 ggtggaatca atggggaaca tacctgctgc ctcaccgatg cccagcacct tttctccgga
     3301 cagcttccag tgaatgtatc ctgccagggt ggtgaagaat gcgatatcct tcacatgatc
     3361 ttctccgctc aaaatagcct gatacagatg tgcaatgctc catctctggg gaatgttgaa
     3421 attaaatacc gggatcagct tcctgcaggc ttcggatgtc tgtgtatttc tccaggtacg
     3481 gaaaggcacc agaagttctc cggctgcatt gaacggcata tagccgtgca tcattccgct
     3541 gaatcccaga gagcccaggg tggtcagctt ctcaccgtat tttgcctcta catcgtctgt
     3601 caggctcttg tagcatccct gcaggccttt ccagatatct tccaggctgt aggtccagat
     3661 gttgttctcc agacggttct cccagtcgta ggttcccatg gcgatgggtg cgtggctctc
     3721 atctaccagt accgctttga ttctggtgga accaaactca ataccgaggg ctgtttttcc
     3781 tgttctgatc gcttctttta ctgcttcgtt cattgtgttc tctccttttc acttctttat
     3841 ttatctcatt ttttatttcc gttttcagtg cattccgcaa cagattcccg ctctacgaca
     3901 cgggtgtcaa attcgtaagt caggtccttt ttcctgcccg cgatcatctg taacaatgtc
     3961 tccgctgctt ttttccccag attctccttg gggtgcggca gggaagtgat gggcacctcg
     4021 ctgtgtctcg ccagatccga atcgtcgatg ctgatgactg acacatcttc cggtacccgg
     4081 atattgcgtt ctgtcaacat ccgaatcaac tgaaaagcaa tctgatcatt gtatgccaga
     4141 agggcggtgc attcctctac ccgattcagg attctgtccc ggcagtaacc aagttgcttc
     4201 gattcgtccg tgtcgatcca caccatccgg gaatccgtca cagtgagccc ggcctcttcc
     4261 atggccttca gatatcccag ataacgctgt ctgccctgac cgtcatccag cttcaggatt
     4321 gccccgatct tctgatgccc cttgtcaatc agatatctta cggccttgta ggccgcctcc
     4381 gcatcattta acgatacatg cggtgcctcc agttcgggat aataggtatt gaaaaacaga
     4441 agcggtatct ttctggccat caggtgccgg tacagattca ggttcggatt cggcaatccg
     4501 ctctttgtcc cctccacgat cactccggcc acatcgtccc gtttcaacag atccttcaga
     4561 atacttttct cccggtccag cgtgttgttt gtgaaataga tctggacaga aaacccgctt
     4621 tcaaataaag tattctgcat tccctgtaaa attctgggga aaatgtagct gtccacgtag
     4681 gtagtcacca cggcgatgct gttgcgctgt tccagattct tctggcggtc aaagctcaca
     4741 taagtgccac tgccacggat tctctgcacc agcccgtcct cctccagcgt gccgatagct
     4801 ttccgcaccg tctgcctgct gatatggaac tgttccgaca gctgattctc cgagggaact
     4861 ttctctcccg gacctagttg tccgtctgat atctgttcct gtatgtaatt aatgaggttt
     4921 tgatattttg tctccataat cactttcagt ataatcagac caccgtacaa agtcaataca
     4981 tcttttacct gttttaggta catttgtata tagttgtttg gtgtacaacc ctgcagtaag
     5041 tctatcccgt ggagtattca catatactct taccaacact ctttttcatc aggtaatcat
     5101 cgtgcgtaaa cgtccccctc tctccttccc acaactactc gtatgtatta gtcttctctg
     5161 cgccctcacc ggtgctctga cccttgtagc ttcccatacc tccccggata gacgttttga
     5221 gcaattcact tcccaacttt ttcaggagga aatgactggc agtaccctga atatgcacta
     5281 caccattgcc gatccaaagg cctttggcat ctccgaatat gaacctgtgc ttccgatcta
     5341 ccactcctac cagccggaag acagcaaaga acactgttcc gacttgttgc accggcttga
     5401 ccgtatagac cctgacagac tttccccgga aaatgcctat acttaccggt tattgcatcg
     5461 ctctctggaa aatgatctgg ctctggcaga ttttccttat tacaatgagc ctctgtcacc
     5521 ttcctccgga atgcagtccc agcttcccgt tctgctggcg gaatacacct tccgcacaaa
     5581 acgggatgtc acggattacc tggctctcct ggatcagata gatgactatt ttgcctccct
     5641 gcttctctac gagcaggaaa aagctgccgc aggcttcttt atgcctgcct gctcctcaga
     5701 aaaagtccgg aagcaatgtg acaccattgt cacaaccgaa gaactggcac agggaaccca
     5761 ttttttacag actacttttg aagaccgcct gtcagagcta cagaagcagg gactttttac
     5821 accggaggag actgcctctc tgattgagac caacgaccgc ctgcttgcca cggtggtgca
     5881 gcccgcctat gccgccctgt ccgaagggct tctttctctg gaaacttcca ccaatgccga
     5941 ttccactgca tccgaaacaa ctgccaatgc tgcttcgggc aaaaacaaca gtgcccacaa
     6001 tgggctcccg aaagggctcg ccctgctgcc ggacggaaag acctactatc tccatctgct
     6061 gttttccgag accggttcct cccgttccga aaaagaactg gtgcagatgc tgcttgtgca
     6121 atttcagaaa gagcaatccg caatccgcaa tctggcttcc cagtcccctt ccctgattac
     6181 actgctttct gaagaaaata cggcggtttt ccctctggca gaaccggaag aaatgctctc
     6241 ggatctacag gctcgtatga aaaatgactt tcctgtcagc agtcccgttc ccaccgtcac
     6301 tgtcaaagat gtggtcccca gtctggaacc ctatagcgca ccggcctttt acctaaccac
     6361 gcctctggga gattccgaca ataatgtcat atatataaac cgccggaact cccctcaggg
     6421 actggagctc tataccacac tggctcatga agggttcccg ggtcatctct accagaccgt
     6481 gtacagcaac cgcatctttt ccaacatgca cacggatcct gccagaaaac tcatctggta
     6541 cggcggttat ctcgaaggct gggcactgta tgtggaattt ctttcctatg attatgcggc
     6601 cactctgttg gaacaggccg gacagtcgga tgccgcacag tcggcccggc tggagaagca
     6661 tacccgcagc ttgcagttgt gcatgtacac tctgttggat ctcctgatcc acggagaggg
     6721 tgccggatac gatcaggtgg cagaggttct tgggaaattc ggtattgatt ctcccgggac
     6781 ctgcgaagca atctacacct atatcgcaga agagccctgt aattatccga aatattacat
     6841 cggttatctg gaaattctgc agctgcagga caccgccaga agccactgga aagatgctta
     6901 ctccgacctt agattccaca ccttttatct ggaacaggga acttctgatt tttcctcact
     6961 ggaagacctg cttttaatga caaattaacg tttttgtgat aggataaagt gtacggatca
     7021 tcaggttgga ggaacccatg acacagttta caagcaaggc tatcgaagaa tcctttatcc
     7081 ggctgctgaa tgaacggccg ctggataaga tcactataaa agacatcgtg gatgactgcg
     7141 gtatcagccg taacactttt tactatcatt ttcaggatat caccgctctg ttggaacaca
     7201 ttctgaatgc agatgtggag cgggtactgt cacagcatct ggatatggac tcctgggagg
     7261 acggctttat ctccgctgcc cggttcgctc tggagaataa gcgtctggtc taccatattt
     7321 acaattccgt cagcagagaa cgggtggagc gctaccttta cagtatcgcc ggagaggtca
     7381 tgcggctgta tgtatccagg atcacggaac aggtggaaca tgcggctcat aaaaaagtat
     7441 ttcccgaaga tcagaaaatg gtggtagatt tttacaaatt tgccctggtt ggcatgattt
     7501 tagactggct gaacaccgga atgaagaaag atccggaggg actgatccgg cgtgtcggcg
     7561 agattttcca cggaaatatc gaagcagccc tgacccgtgt ggcccgttaa tttggacatt
     7621 tgtccatgtt tgcacatatt ttggattatt taagagcaat gcgcaatttt tgtgcattgc
     7681 ctttttttgt ccgttttcaa gactggattc cttgcataca ataccaattg agaaagaatc
     7741 gacaacgaaa ggagccctgt tttatgaaat ttggtaaatg ggtcgtaaaa tgccgtattc
     7801 ctattctgat actggcagtt gcactactgg tcccttccct gattggtatg atcatgacgc
     7861 ggatcaatta cgatatgctg acttacctgc ccggtgatat tgacacggta gtgggtcagg
     7921 atattctgat ggatgaattt ggaaaaggag ccttttcctt cgttattatc gagggaatgg
     7981 atcccgagga tgtctcctcc ctccgggaag atatttccca tgtagatcat gtggacaccg
     8041 ttctttggta tgacgatttc gcagatgttt ccgttcccat ggaaatactt ccttccaaac
     8101 tatatgacgc tttcaattcc ggtgacagca ccatgctagc gatcttcttc gatacctcca
     8161 cctcctccga cgatactatg gaggcgatca cagcgatccg cagcattgcc ggcaagcagt
     8221 gttttgtctc cggtatgtcc gccatggtca ccgacctgaa ggatctgtgc gaaaaagagg
     8281 aaccgatcta tgtcggtatt gccgttgcac tggcctgcgt agccatgatg atcttcatgg
     8341 acaactggat cacaccgttt gttttcctga tgagtatcgg tatggcaatc ctcttaaata
     8401 tgggtaccaa ctatttcctc ggagagatct cttacctgac caaagcactg tccgctgtcc
     8461 tgcagctggc tgtaaccatg gactactcta ttttcctctg gcacagctac gaagaacaga
     8521 agagtatgta tgaggataac aaggaagcca tggctgtagc catcaacaac actctgacct
     8581 ccgtggtcgg aagttccatc accacggtag ccggatttat cgctctgtgc tttatgagct
     8641 ataccttagg tctggatctt ggtattgtca tggccaaggg tgtcatcctg ggtgtcatcg
     8701 gttgtgtcac aacgcttcct tccatgatcc tggtactgga taagttatta cagaagacca
     8761 gtcacaaatc cctgctgccg gatatgggaa aagttgcttc aggtatcacc aaagtattcc
     8821 cggtattcct gatcctgttc ctggggctga tacttccttc ttatctgtct tataaggcaa
     8881 ccaacaatga agtgtattac gatctcggtg aaacccttcc cgaagatatg gcctacgtgg
     8941 tagccaacag caaattgcag gaagacttcg gtgtaggtgc cacccatatg gtactggtaa
     9001 gcaccgatgt atctgacact gacgtacgtg ccatgatcca cgagatggaa aacgtcgagg
     9061 gtatcaaata tgctctgggt ctggaatccg tagtcggccc tctggtacca gaggaaatgc
     9121 ttccggaatc cgtaaaagaa gtattaaaga gtgatgactg ggaactcctt ctggtgaact
     9181 ccgaatataa gaccgctacc gatgaggtaa atgcccagat caatgagctg aatactattt
     9241 tgaaaaaata cgattccaag ggcatgctga tcggtgaagc tccctgtacc aaggatctga
     9301 tcgagaccac cgacgaggac ttcaaagttg taaataccgt ctctatcgta gcgatcttcg
     9361 tgatcattgc tctggtagaa aaaagtatta ccctgccgct gatcctggta gctgttatcg
     9421 agttgtccat cttcatcaat ctgggccttg ctcacctgac aggaacgtca cttcctttca
     9481 ttgccccgat ctgtatcagt acgattcagc tgggtgctac tgtggactac gctattctga
     9541 tgaccacaag atacaagcag gaacgctacg aaggcaggga caaacgtgaa gctgtcacca
     9601 acgccctgaa ggtatccatt ccttccatta tcgtcagcgc catgggactg ttctccgcca
     9661 ccttcggtgt tgccctgtat tccgacgtgg acattatcag ttccctctgt gatctgatgg
     9721 caagaggcgc tattgtaagt atgtttgctg ttatcctgtt cctgccggcc atgttcatgc
     9781 tgttcgacaa ggtgatctgt gtcaccagca tcggcttccg gaacaagaat gcagaaccct
     9841 ccaatacatt gaaagaaagg acggcataag attatgatca atttgaaaaa acaagcctgg
     9901 agaaccgggg ctctgtgcct cgccggtgtg gtagccttaa gtgttaccgg tgtctccgta
     9961 tatgcaaaaa cttccgaaaa gaaagagacc gaggtcaagg agcagatccg ggatgccgta
    10021 gatgacatct ggaaatccga agatacctcc gcagcctcca ttgatgagac cgtctatgtg
    10081 atcctgaatg cagacggaac ccagcagaag atctacgtca gcgactggct gaagaatcag
    10141 ggagaaaaag attcctacac ccagagcaat cccgataagg atgctcccat ttccctgaaa
    10201 gttacttata cgttggacgg caaggagatc tccccatcag atctgaacgg ccagagcggt
    10261 catgtagtga tccgctatga ttacacgaac gaattatatg agacccttga gattgccgga
    10321 aaagaggaaa agatttatgt tccttttgcg gtaatgaccg gtatgatcct ggataatgac
    10381 aatttcagca atattaaggt gtcctcaggt aaggtcatca acgacggcag ccacagcgtt
    10441 gtcaccggta tcgtcttccc cggactgggt acgaacctgg atatggaaga ggatttcgac
    10501 gattatctgg agattgaggc cgatgcaacg gatttcacca tgaatgaatc ctactgcatc
    10561 gccaccaatt ccgtcttctc ccgtctggat ttcagtaatg tggatgatct cgatgacctg
    10621 acagaggcaa tggatgatct ggaggatgct accaacaaac tgttggacgg gacttccgat
    10681 ctttatgatg gtgtcacaga gctttatgac aaatccggtg acttgcagga tggtgtaaaa
    10741 gaactgtccg atggttccaa cgacctccgg gatggcgctt acaaagtagc tgacggtact
    10801 gtcactttaa gaggcggtgt cggttctctg cggagcggca tcggtacact gcaggagggc
    10861 accaagaccc tgttagcagg tacacagagc ctgcagtccg gtactaatga tctgaagaat
    10921 ggtctcggtc agctgactgc acagaatgat actttgaacg gcgctgccgg ccaggtattc
    10981 gatactttgt tgaaaactgc agcttcccag ttaacggctg ccggtctcac gcttccggaa
    11041 gaattgacaa tatctaatta tgcgaccata ttaaatgctc tgtatcagca aatggagcct
    11101 ctctcggatg ctgacgttgc tctgggatat gtcctccagc aggccggact gacgattgaa
    11161 aaatatcagg gtatgattca ggcatcccag agcgtatcct caggtgatgc cgcacttcag
    11221 gcaaatttga aaaccattca ggataaagcc acaagtcttt cacaagctgc cggcagcatt
    11281 gctgccttga aagcttctct ggatggctat aatcagttct acaccggtct ccagacttac
    11341 accgccggtg tagcatccgc aagtgccggt gcttccaaac tggctgacgg ttccgcccag
    11401 gtcgttgccg gtgcttccca gttaaaggat ggcgctgaca aactggcaga cggaagtaaa
    11461 gagctgtatg atggtgctga tactttagta gaaggtgcca gagatctggc ctccggcagc
    11521 ggcaaactgg ctaacggcgt aaacgagctt tccgatggaa gcgataagct gatcgatggt
    11581 atcagtcagc tgaaggatgg ttccaaggaa ttaaaggacg gcatgatcga atacaatgac
    11641 gaagccatca gcaaactgac tgatatggat accgatgaac ttcaggaagt gatcgatcat
    11701 atgaaagcta cctcttccgt atccgaaaat tacaactcct tcaccggaga caataacggt
    11761 atggacagca gtgtgaaatt tatctacaag atcaacgctg taaaataaaa catcacaagc
    11821 ccgtaacttg acaaagcccc tctccttttc tatacttgca aaaggagagg ggcttttcgc
    11881 ttcctatatt ccgcttctga taacagaaag gcatacaaat tcccatggaa caactattat
    11941 tcagtctcaa tgcaacgatc cccgtattcc tggtcatggt gatcggttat gttttacagc
    12001 agctgcatgt taccaacgac tcttttgtca aaaccctgaa ttcattcaat tataaaatca
    12061 ccctgcccgt acttttgttc aaggacatgt ccactgcaga tttttacagt gtatgggata
    12121 ccggttacgt gctgttctgc ttcttcgtga ccctgttctg catcgtcatc acctgggtcg
    12181 tggcaggcat tttttataag aacaaatccc gtctgggaga gcttgtccag gcaagctatc
    12241 gaagcagtgc cgctgtcctg ggtatcgcct ttatacagaa catctacggg aactccggca
    12301 tggctccctt gatgatcgtg ggtacggttc cactgtacaa tatcgctgcc gtgctgatcc
    12361 tgtccttcac cggaccggat gcgaagggct tcgataagga atccctgctg aaatccctaa
    12421 aagggatcat cacgaatccg atcattatca gtatcgccct gggcatgctg tccagcgcct
    12481 gccggatcag ttatcccgtg atcatttcca agaccatcag caatattgcc gtactggcta
    12541 cccctctggc cctgatcggt ctgggtgccg ggtttgaagg ccggaaagct ctgaaactgc
    12601 tggctcccac cggagtagcc accgtgttaa aactggtggt atggcctgct ctattcctgc
    12661 cattggccgt gcatttcggt ttcaccggag aaaaactggt agcgatcctc attatgctgg
    12721 gctctcccac cactgtcagc tgctatatca tggcaaaaaa catgaaccac gaaggaactt
    12781 tgacttccag cgtggtcgtt gccaccacgt ttttaagttc cgttaccctg acggtgttct
    12841 tgtttatttt gcgaagcttg cagttgattt agttatatga tgcccacgaa ttgttccatg
    12901 ctgcgcactc tcacaattct acccaatatt cgcctatcgt gggattatca tcacattttt
    12961 cccgcaagga aaatacttgc cactgttccc gctaacgttg ccagcaatgc ccccggcagc
    13021 gtgtagcggg tttttgttac ttttgctgcc agaaaataca cgctcatggt gtaaaaaata
    13081 gtctcggtac agctcatgag gatcgaagtc agcatcccgg cataagaatc cggcccacag
    13141 gtcttaaaaa tatccagtac cagcccggtt gccgcagagg aagagaacat tttcacaata
    13201 aggagcggta tcagctgtgc cggaagtccc accgcctgtg tggcaggtcc gatcagatgc
    13261 cccagcaggt caaaaaagcc ggaggtcctg agaatcccca ctgccatcat caatcctacc
    13321 agtgtcggaa ggatctccac aacgatgcgc aggccctctt tggccccttt caaaaaagtc
    13381 tcatagacct tcacccccga gaccatccca taacccacga tccagaaaat caccatggga
    13441 atcataatat ctgacatgtg tgtcaatgcg ttcatcatga tcctcacctg tccttccatt
    13501 tacagtaaat gatggcaatg atcgtactga acagtgtggc caccagcgcc ggagcaatga
    13561 tcaccgccgg attggcgctg ccgtactggc tgcgataggc aatcatattg accgggatca
    13621 gctgtaagga tgaaatattc agaatcagga aggtgcacat ttcgttgctg gctgccgtgg
    13681 cctctgctcc ataccgtcga tcctccgatc cccgtcgtcc cttctccgtc ccctgccctg
    13741 ccactggtcg taactgcctg ttcacaggct gcatcctctg ctgctctatc cgtcgctgtt
    13801 ctgccctctg tttctccagc tccgccagct gctccatggc ctttaatcca gccggcgtgc
    13861 aagcccagcc aagccccagg acattggcaa tgagattcgt ggttatgtat tcccctgccg
    13921 ggtgatcctc cgggagcctg gggaacatga atttcagaaa ggggcgaatc ccttttgtca
    13981 ttttcgcaat cattccagac tcctgcgcga tctccatcag tcccatccac aatgccatga
    14041 cccccgccat ggtgatgcag aggctcacgg cctctctggc actgtctaac gcgccccccg
    14101 tcagtgcgct catctgcccg gtacagacac catagatcac gcctaacagg atcattcctg
    14161 cccagatata gttaagcatc tcataaaccg cctgctattc cttcgtttca ctatatgcgg
    14221 gcagcggtat gtcctatgca gcacaggtca cctttgcacg ttgctcccga cgggatgtta
    14281 catgcaaaat gagccttgcg ggcagtgctc cctgccagat tcaacgcttc ttatcaggtg
    14341 cactaaaata aacctcccgg gaataaattc tccgggaggt ttactctaaa ctacatttat
    14401 aagttatatt tattaccttc ggatctacag agttccctgt gtgaacaggc ttctcacatg
    14461 tgccacttct tctgcggtgg ctttctccgc cggcacataa taagacttct ctctggcgat
    14521 ctggtacagt tcctcctggg actgctcaca ggccgtacgg aactgcttca gtgtctgttt
    14581 taactcctta ttctccgact gggcgatcat tcccgcataa cgggtcagct cgccgttgat
    14641 tccggccaga gtatctgcta ccattgtctt ttcctgcatt ctgctgcctc cttactgcaa
    14701 aaacttcata agctgctgtt tgttctccat cgcgctgcga gcaccctttt caaaaaactg
    14761 ttttaccttc gggtcctccg cttccttggc gtactcctgc attttgcagt gggtcgtgtc
    14821 aaaaccgccg atcagatggc gcagattctg cagatctaat tctgaaatat cactcatgct
    14881 gttaacctcc tttttcttat ttgcgaggtt agtatgcgta gaatattttt ttatattcaa
    14941 taaatatgat acctacgaat tgttccgtgc tatgcacttc cacaattctc cccactattc
    15001 gcatatcgtg ggattatcat ccctctttta tgctcatatt ggggacgggt cccaaggtct
    15061 ttcacccagc catgaacagg ctcatgcggc tttagacctt ttaccctggt atcattaaat
    15121 aaaattggca aatatagata ccgaaatcac ggtcaaaata ccgcagacca cgatggacag
    15181 actgctcatg gcgccttcga tctcacccag ttccatggct tttgcagttc ccagcgcatg
    15241 agcactggta ccgatggcaa gacctttggc aataggatcc tcgatcttac acaggcggca
    15301 gatcgtatct gccagaatat tacctaagat tccggtgaca atgatgacaa ctacggtaat
    15361 ggtcacgatc ccacccattt tttcagaaat ccccatacca atagccgtgg taatggactt
    15421 cggcagaagc gttacatact gctcatgatt cagaccgaac atcaggctta aagcaaaaac
    15481 gctcaccatg gcagtcagca cgccggcgaa gattccgccg aaaatggcct tgggataagt
    15541 ctttaacaac tccagctgac gatacagagg cactgccagg ctgaccgttg ccggagtcag
    15601 gaaaatgctg atatactgtg cgccgtttgc ataggtctca taatctatat ggaacaataa
    15661 caatactgcc acgatcagga ttacggagat cagtaaggga ttggcaatgg cttttccagt
    15721 cttctgtttg atccacagac cgatctcgta tgtgaccagt gtaatggcaa taccgaaaaa
    15781 aatggactga cttaatattt ccttcattta tctgtcctcc ttatcatctg cggcagcttc
    15841 cgttccgacc gtctgcatgg ctgcggcacc tttgtttttc tgcccccgga taatatgctg
    15901 ggatacccgg cctgtcacaa tcatgatcag taccgtggat accaccgtga tcacgcagat
    15961 gggaagcagc atctctttta aagcatccac acttgccatg attcccacgg tagcgggtac
    16021 gaacataatg gacatggcat ccacgaggaa atccgctgcg gtctccacct tttccagtgg
    16081 gatcagcttc gtgatcagcc ccaccagcat gatcaccaga ccataaacgc tggctgcgat
    16141 gggaaacggg atcaggatct ccagaagctc tgccaaaaag gagattccca ggataatcga
    16201 aaattgtttc acatatttca tgatattgtc accctacctg ttaactttag tatttcattt
    16261 ctttgatgtc ggggtcaaaa gcccccgact ttgatgccta cggattgctc cgtgctacgc
    16321 actcccacgt ctccgctccg cttcggtccg tgctagacat gtgccactgg cacatagcac
    16381 cctggcatca tcattatgtt gcacacaaac aaaagaagct gcgtactatt tttcagtacg
    16441 caacttcgta ttatactact cggtttttac tttcacaatc agcattctct accatttaag
    16501 tagacttttt gaccttgctt tcgtgaattt ttaccttaca tctggtgtct taccaccgca
    16561 tattcgtcat ccagtcgaaa gtagggacat tcctgctggg ttccggataa aaagcggtac
    16621 atttcatctt catccagatt caccatgcag caatagcatt catagtcttc gtcatattca
    16681 taattgacac aagcgtcaca attagtcatt ttccctctct ccgcatatgc ctttcatgga
    16741 tgtatcctac attatcctca tatgcttttg tctgttttgc ttcggtttca ttatggtatt
    16801 ttcactgttt catgagttgc catgcaagta ctgataaatt tcccgttttc cgacacccct
    16861 gtcttttgcc acctgcttca tggcggattt attatccatg ccctgctctt cgtaataagc
    16921 catatgttct tcaatgctca tggattccca ggatgcgatc tcttcctgtc gtttctcttc
    16981 cggacttttc ccttccacta ccaggacata ttccccgcgg ggctcctctg tcttgtagta
    17041 atccaacgcc ttttccaaag tggtggggaa tacggtctcg aacttcttgg tcagttcacg
    17101 gcacaacgtg atccgccgct cccccagtgc cgcatacagt tcctccagtg tccggatcag
    17161 atgatgggga gcttcataga ggatcatggt acggctctcg gtcttcagtt cctccaggat
    17221 cattttcttt tcctttttat ctgcaggcag aaatccttca aagcaaaacc ttctcgtact
    17281 gagtcccgac aaagtcagcg ccgtaatgca tgctgccgga cccggcagag aagtgacggg
    17341 aacgctctgc tcctggcaca tccgcaccag tacttcaccg ggatcggaga tcgccggggt
    17401 accggcatca gtgatcaacg caatgttctg tccgtttaac agctgtccta ccagctggtg
    17461 cgctttttct actttattgt actcgtggta actggtcata ggtgtgtgga tgtcgaaatg
    17521 attcagcagc ttgatactgt tacgggtatc ctccgccgcg atcacatcca ccgtctgcag
    17581 ggtctccacc acccgcggtg tcatatcccc cagattgcca atgggcgttg cacacaaata
    17641 taaagttccg ggcatgagct cctgcctcct ctccaaatca tagtagtcta taactattca
    17701 aagccatgat atcatgtcac tatgtatggc gaatgctcgc attcgtaaga acatttgaca
    17761 taattatacg gtgaatggtg tgctgaatag ttacaatatc ttaactatct aatatccata
    17821 tatatcatat atttccggca tatatactcc cggcgactga tatacgatca gcggcttctc
    17881 caccgtcatg cggggtttgc cgcctcgcac cgcctcgatg agcaccatat tgggctcctt
    17941 gtcgatatag ggatatacaa gctgcatccg cttcggctcc agcttgtatt ttaccagcat
    18001 ggtcatgatc tccgccagcc gaaaagggcg atgtaccatg taaaaatgtc cgccgggctt
    18061 taacagtttt gctgtcctgc tgaccacatc ctccagcgtg cagagcactt cgtgtctggc
    18121 gattgctttt ggttcctccg ggttcgtcaa tccatgctgt ccgatcatat acggaggatt
    18181 gcaggtaata cagtcaaagg aagcagccgg gaaaatctgg tctgcttcct tgatgtctcc
    18241 tgtcacgatg tcaatgcgat cctgcaggtc attcagtgcc acgctgcgtc tcgccatatc
    18301 tgcgctctcc tcctggattt ccagtccggt gagatgagaa cagtggtatt ttgcctccat
    18361 cagaagcggg atgatcccgg tgcctgtgcc aaggtcaagc atcgtatcct tttctctggc
    18421 atgggcaaat cctgtcagaa gcaccgcatc cataccgaaa caaaatttct ccggattctg
    18481 tatgatctga taaccgttcc gctgaaggtc atcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnaacaaaa tttctccgga ttctgtatga tctgataacc gttccgctga
    18661 aggtcatcaa ttcgttcttt atccctcagt tcaaccttca ttcgtgttct ccggttttct
    18721 tccgttaccg ttattaccgt ctcttctgcc gccgtgtctc ggtcgtctgc ggaaggaatt
    18781 gtccctctga gttctgcgct cgccttcctg accgttacca ccttcctgtt gtgtctggct
    18841 ggcgggacgg aactgatctg ctctctgtgc attgtccctt acctggtccg gacggttctg
    18901 gggacggttc tcgcggcgtc tgtcacctcg attattatcc ctgcggttct cacttttgtt
    18961 gccattgtca ccaccacggt tgtcattgcg gttctgattc tcgccgttac ggttattttc
    19021 cccgcggtta ttatttctgc ggttgtcgtc cctgcgtccg tcgcgtctgc ggttttctcc
    19081 acggttgtca ccacgattct ctccgtcagt tctctgctct ctgcggtcct gacgctgctc
    19141 cgcacgtttt tctgcgcgtt cttccgcttc ttccttctcc tcgatctcct ctaacttctc
    19201 gagttccttc tgttcttcct tagacagttc ctgaccgccc tttttgccgc ggcgtctctt
    19261 gaattgtaac gtgtctgcct cgtactcctt cagttccttc tcgtcaccgg cttctaccag
    19321 taccttgacc agctggcgca gtacgttgac gctctgtacc tcgccgtgca gtccgtctgc
    19381 cgtctggaca tagtcaccca caccgggaag cttgcggttc agttcctcgt aagtgtcttc
    19441 ctcatttttc agacagcaca tcagtctgcc gcagactccg gagatcttcg tcggattcag
    19501 ggacagattc tgctccttcg ccatcttgat ggataccggt acgaaatccg acagatagct
    19561 gtggcagcac agaggccttc cacagatacc gataccgccg cgtatcttgg tctcatccct
    19621 gacaccgatc tggcgcagct cgatacgtgt tttgaacaca cttgccagat cctttaccag
    19681 ttcgcggaag tcgatacgtc catccgccgt aaaatagaat aataccttat tgttatcaaa
    19741 cgtatactcc gcatcgatca gcttcatttc cagaccatgc tttaatatct tctctttaca
    19801 gatcttgaag gcttccttct ctttttcttt gttggcagcc tcctgctcga tatctctctc
    19861 tccggcgatc cgcatcacag gcttcagggg ctggatcacc ttgtcatcct ccacctcatg
    19921 cactcccgca acgaccgtgc cgaactctat gcctctggcg gtctccacga tcacgtggtc
    19981 gcctctcttt atctcaagct gcaacggatc gaagaaatat accttgcccg ctgtacgaaa
    20041 tcttacacca attacttttg tcatatctat ctacctcact gatgccgttg gcatcttcta
    20101 ataaattaaa ataacgtagc tattcagcac ctcatcgcaa aatagcagct gcctgcgcga
    20161 aatgcgcctg caactgctat tttagcatat cttttcgaaa atgactactc tatttcttct
    20221 atcgcttacc gctttaggat ctgagttcca cacccagctt gttgttcaga ttcttcaaga
    20281 tcttcttcac gtagccgtcc actgcctctg tggtgaattc ctcgtccttc ggagcgaagg
    20341 tcacggtgaa ggccatggac ttcttctcgg gtcccacctg taatccctca tagacat
//
LOCUS       DAWEJW010000050        20182 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1043_length_20182_cov_55.776122, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000050 DAWEJW010000000
VERSION     DAWEJW010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 20182)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20182)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20182
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1043_length_20182_cov_55.776122"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10670..10769
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5033 a         5488 c         4039 g         5522 t
ORIGIN      
        1 tttatttcgc aagtaattct gcaatcttct ctcactaatt ttccagcatg aaatcacaga
       61 atttttatat ccatgttcat cccccgaagt acataatctt aatacaatct gtaaaaatcc
      121 ttcttccgtt tgtattcttt taataatcag tcccgtatat gcatgcttat ccctaataat
      181 ataatccggg gtacttattg cctcctgtat atttttcaat actttattat atgcctctgg
      241 atgtctgtcc ttaatatgtt gaatctgatt gtctgtgata ataacctctt cggtaataat
      301 atcctctgtt atacatttat atattttttg atttattttt cctatgctat acacttcact
      361 cttttcacca ccttcatttt aacttcatgt ttctttcttc cattcacgtc tctgggaaaa
      421 cgactataga tcgctagtca acgatatgag aatttgtaag agaaatattg ctcttattgc
      481 taagataaca tgaacggcaa ttttttacaa gaaaaaagac gactgcactc acatgcaatc
      541 gtcttctatc ttctttactg ttccagttta tccgcatatt tgatacggta gattctccgc
      601 agggaatcat tgatgcgttc ctcggaaatg gtgccgttct gtacagcctc caatactccg
      661 ttgtatgccg tctcaaaatt ctccggtgca tagatcatat cacagcctgc gcgaagtgcc
      721 agtaccgcag cttcatctgc tccatagtaa ttggagattg cagcctcatt catggctccg
      781 gacacgatca caccgcggaa attcagttca tttcttaaaa tatccgttac caccgcgctg
      841 gagaacgtgc agggggtatt gtctccggtc agggaagatg ccgccatatt actgatgcgg
      901 atcattttgg tcatgccatt gtcgatacaa gtctggaaaa ccacaaattc attggctctg
      961 aaatcttccg cacttctgtc tgtagatgcc atgccgtcct tcgtgctctg ggtactgctg
     1021 ccgataccag ggaactgtcc gatacatgcg gtcaccttct gctcctgtaa tcctgcctgc
     1081 atatatccta caaaggatgc cacattgtcc gcttccgatc cgtaagaact gctgcctgcc
     1141 gcatttccat ccactacact gagatctgca gaaggtgcga aatcaagatt aaagcccaac
     1201 tctgccagat aagttcccac cgtggttccc gctaggtacg cattgttggt atctccggta
     1261 gccccgatgc tctggggtgt gtccacttta gtgccaaggc ctgccgcagc taccgcatct
     1321 gtatcgcttc cctctccgtc aatggcaata aacaggggat agctggtata taatttggta
     1381 ttatcgatca tctgcttcag ctgatctgca gactggatat tcttcgctgc atatacgatt
     1441 ccacctaccg ggtatttcga caaagcatcc ttggtaccgt ctcccgcctg tactgcagca
     1501 gacacaccgg tgatcgattc cggcgtcgta atgaataatc ctgctacctt atcttccaga
     1561 ggcatgttct ggatgattgc ctcattaata atggtatcca gcttctgctc atccgtgagt
     1621 tccaccactg tctcctggct ttccgtcggc gcctggattg tctcctcatc cgaaaggatc
     1681 tcatccacct tgttctgctg ttcttcctga ttacgactca tgctggtaat cttgcttaca
     1741 gcaaaagcaa ttcccaccgc cagcaacagg atcataatac ctacggccgt atatgcaatg
     1801 atctgatttc tctgtcttct cttatgtctg aattctcttt tgtcctgctg attctgttcg
     1861 tttgtgtcca ttttcataac tcctctatct gtactattct tctattgtac atgatatcgg
     1921 caggtttttc cactggaaaa ttgtacaact attcagaacc tcattcgcaa aataaaaaat
     1981 ccaatatgga cttacaaatg caagcatttg acaaccatct tgatttttta cttggctctg
     2041 aatagttata caacattaag agagcgtacc gatcattact tcaatcagta cgccctctca
     2101 gactacaggc tccaatggct tgccctctct ttcttaaatt tgatcttcca ttttgtcttt
     2161 tgttctcttt ccacttttct tttgtacgga tgtactgtaa tgtgagataa ttcttatctt
     2221 ctgttgttca ttactttttc ttctgtgtac ttgatgttct ctatgtaaat tggttctctt
     2281 tggtagttct attcagttgt acatcatgga agattgtggt aggactttaa ttgttaggtg
     2341 gactgtacct ctctttctat agattctttt gtacttctct cccaaccttc tttgttgggt
     2401 tgttccgttt ctcttttgtt aagtctaata taatacatgc ttgtacattt gtcaacacat
     2461 ttctcgaaat attttaattt attttcaata tttttattgt gtttgtatat ttttacaatt
     2521 ttcgactttt ttccatatat tatctgtctt ttttcaaaaa aaggcaaggg caaagggcgc
     2581 gccgctgccg gggaaagtgt aacgagccat gcctgcatcc gccaaaagcc ctggcgggct
     2641 ttcgtcggat gacggctgtc gccgtataaa gtcaaaaaaa ctaccactcc ctggtgagca
     2701 agctccccag ggagtggtag tttccttgcc tttgcatggc tcgttacacg gcaaactggc
     2761 tgttatacag attcgcgtag aaaccgccct ttgccagcag ggtctcatgg ttgccctgtt
     2821 cgatgatgtt tccgtctttc attaccagga tgacatcagc gctctggatg gtggaaagtc
     2881 tgtgggctac gatgaagctg gtgcggcctt ccatcatttt ggcgaatgcg ttctggatct
     2941 tgatctcggt acgggtatcg atggaggagg ttgcctcatc gaggatcagc atgggaggca
     3001 ggcacagcat gactctggtg atacacagaa gctgtttctg tccctgggac aggctgccgc
     3061 cgtcttcggt gatcacggtg tcataccctt ggggcagtcg cttgataaag ctatgggcat
     3121 gggatgcctt ggctgctccg atgatctctt cctcggtagc gtccggttta cccatgcaga
     3181 tgttgtcacg gatagtgccg gaacgtaacc aggtctcctg cagcaccata ccgtaattgg
     3241 tacggagact tcctctggtg atatcccgga tgtcggtacc atccacctta atggacccgg
     3301 agttcacatc gtaaaaacgc atcaacaagt tgatcacggt ggtcttaccg catccggtag
     3361 ggcctacgat ggcaactctc tgaccggggg taactttcag gttaaagttc tggatcagct
     3421 tcttctccgg cacatactgg aagtatacat tcttcagatc cacattaccc tgggcatctt
     3481 ccaatactct tgcatccgca gcatcgggca cctgaggctc ctcgtcgatc agatcgaaga
     3541 tacgtccggc acagacaaaa gcattctgca attctgttac cacgccggag atctcgttga
     3601 agggcttggt gtactggttc gcatagctta aaaagcagga cagacggcct acgctgatac
     3661 cgcccttgat cgctacgagc gcaccgaaca caccgacacc ggcatatacc agactgttaa
     3721 cgaaacgggt cgccgggttc gtaatggagg agaaaaagat agctttcaga gagcatttct
     3781 gcaatctgcc gttgatctct ccaaacttgc tcttcaccgc ttctcctctg gaaaaggtat
     3841 ttacaatttt ctggttgtcg atcatttcct caatcaggga ggtctgctcg cctctggtct
     3901 cggactgctc cctgaacatg gagtaggtct tggttgcaat gaattttgcc accaggaagg
     3961 ataccggagt gatacatact actaccagtg tgatgggcac attggtcacc aacatgaagc
     4021 ccagcgtaca caggatcgtc agcacaccgg tgaacagctg ggtaaatccc atcagcagac
     4081 cgtctgcgaa agtatccacg tccgcaataa cacggctcac gatatcaccg taagcatggg
     4141 catccagata ttttaagggc aggatctcca gcttcgcaaa agcatccgta cggatatccc
     4201 ggatcacatg ataggtaata tagttgttgc aggtgttcat gacccactgg cctactgccg
     4261 tgatgatcat gacgatagcg atttttttca taatggcaaa gatgtcgggc atatccacct
     4321 gtccctcgcc gatgatcaga tccaccgcat taccggtgag gatagggata tataaggtca
     4381 gtaacacggt caggcctgct aacagcaagg agagtaccag tgccggtgta tatttccgga
     4441 tatatttcaa tacccgcttc atcgtctctt tgttattttt cttctgcatt accgaacacc
     4501 tcctctctgc tcaggatact gggaataata aatctcctga tataccgtac aatccttcaa
     4561 caattcatca tgggttccca cacctgcgat ctcaccgtca tccagtacga tgatcttgtc
     4621 cgcatggcgg atagtggaag ctcgctggga tacgataaag gtcgtggtct tatcttccaa
     4681 agtccggatt gctgcacgga gttttgcatc cgttgcgtag tccagtgcgg aggcactgtc
     4741 atccaggatc aggatctcag gctctctgac cagggctctc gcaatggtca gacgctgtct
     4801 ctgtccaccg gagaaattct taccgttctg tgccacagtt gcatccagct taccgtcctt
     4861 gccctctacc acttccgatg cctgtgcaag ttccagtgct ttccacattt cctcttctgt
     4921 agcatccggt ttgccccact ggagattact gcggatcgtt cccttgaaca gaaccgcctt
     4981 ctgaggtacg acaccgatac gcccacgcag ctcctcatca ctcatggtct tgatatctct
     5041 gccttccagc aggatctcgc cctctgtcgc cgggtagaag ccgggaatca ggttcaccag
     5101 agaggtctta ccggaaccgg tacctccgat gatacctacg gtatcgccac ggtttacggt
     5161 gaagttcata tcctgcaaag taggtgcacc ggctccctga taagtcaggg atacatgttt
     5221 gaagtctaag aaaggtgcgc tcttatctac cttatccgct agtttctgat tctgcgctcc
     5281 cacatatgca gcatctgcgc cgatatcgaa aacggaagct acacgttctg cacaggccag
     5341 agccttggtc atggttacga tcaggttcgc cagcttcacc agttctacca ggatctggtt
     5401 catatagttg atgatcgcta caacttcacc ctgactcagg ttaccgatgt tgacctgtac
     5461 cgcaccggta tagattaggg caatgatagc accgtttaca atgacatagg tcacaggatt
     5521 catgcaggca ctgatcttac ctacgaagat ctgcatggcg gacaatgcct cattgttctc
     5581 ccggaatcgc tcttcttcct tcgcttcctg atggaatgca cggatgacac ggacaccggt
     5641 cagattctct ctggtgatgc ccaatacctg atccagcctg gactgcacct ttttgtacat
     5701 agggattgtg gataaaataa taccgaatac cacaatggac aacaaaggaa tcgccactac
     5761 aaagatcagg gcacatttca catcaatggt gaatgccatg atcatggcac caaagacaat
     5821 gatcggggaa cgtaagaaca gacgtaaggt catattgata ccggactgca cttggttcac
     5881 atcactggtc atacgggtga tcatggtgga ggttcccgcc ttatcaatat ccgtaaagct
     5941 caaatcctca atatggtcaa acaatgcctg ccgcagtttg gtggataccc ccactgccgc
     6001 cttggctgcg aaaaactgtg cagtaatgga cgatgccagt cccactacac caaggagtaa
     6061 caagagcagt cccattttcc cgatataacc catattccgg ttggaaatac catagtcaat
     6121 aatatttgcc attaccagag gaaccatcag ttcgaaaaat gcttctagca gtttgaacag
     6181 tggtgccagt atggactctt tcttataatc tttcagatac atcattaatc ttttcataaa
     6241 gtcctcattt cttcgcgctt tccatgtaat gcgcatccgc ttttcatgct tcctatccct
     6301 ttacagcttt cccgttcctt tctttttacg cttctgctgt tccgtaacag accgccagcg
     6361 aatagctatc ctccagaacc cgttcccgga aacatatctg cttttcctgc cagttctgtt
     6421 cgtcggagaa atcttctccc actgcactgc cgatcccctg gccggtgtat ttgccgtagg
     6481 cttcttttct gctccacaat cggtaaaaca gttccgtgcg ctgtgacgca ggcagttcct
     6541 gcagcagttt ccattccgtt ggcgcaaaaa aacgctctgc cgtccgttcc ttatagggaa
     6601 cccatttctg aatgtccgct cccacttctc cgtctcctac cgcgcagacc acatagtctc
     6661 cggaatgaga cagattgaat tttttcggga tattcacgat ctgtggcttc cccagttctc
     6721 catattcgta ggaaaaggat aacgcccccg ccttgtcccg gtgcatccgc tcctgtaaca
     6781 ccgcaagcag ctcatcctct tccagaaaca ggattccggt attctccgta ccgtctatca
     6841 gatccagagc catcttctgc aacagcattc ccgcgccgag actggctgcc tttgctccgg
     6901 tgcttttcag ccgttgtacc ttcttacacc gcaccggatc cacatattct gcagcttccc
     6961 gcaggaatgt ccgcctgatt tcttcctcac ctgggtgcaa atgcaataca taaattccta
     7021 cagccatatt cttctcctga caaattgcca tatataccca acataaaggc tgtcacacag
     7081 aaatcgtgcg acagcctcat tattattctt ccggcttggc ggtgatggcc agatgcagtt
     7141 catccaactg cttctgctct accgtacccg gtgctcccat cataatatcc atggcattct
     7201 tgttcatggg gaaaggaatg atctcacgga tgctttcctc accacagatc aacatgatca
     7261 tacggtctac gccgggagcg attcctgcat gaggaggtgc tccgtaacag aatgcattgt
     7321 acatggcagg gaactttgcc ttcacatcgg cttcgcctaa gcctaccagt tcgaatgcct
     7381 tgatcataat ctcaggatca tggtttctca ctgcgccgga ggacagctct acgccgttgc
     7441 agaccagatc gtactggtat gccagaatct ccagaggatc ctgattgttc agcgcatcca
     7501 taccgccctg gggcatggag aacggattat gacagaattc cagttcgccg gactcttcac
     7561 cgatctcata catcgggaaa tccacaatcc agcagaactc gtagcaatcc ttcttcatat
     7621 gtttctcgct ggctgcaccc agcagctttc tgtatacacc tgctgtcttc tgcgcttcca
     7681 gcttcttgcc tgcggcaagc cctacaaagt caccgttctt aaggccgaga gccttgatga
     7741 cttcttcctt gcgatcctgc aagaacttgg agataccgcc tacgaactct ccgttctcgt
     7801 ctacacggaa ccagaatccc ttgcgtgcgg tctgtacttc tacctctgcg cagatagcat
     7861 cgatctgctt acgggtagca gtgaagccat ccaccacgat agcctttacc gtctgacctt
     7921 cgaaagcagc aaagccacag ccctgcatta cctcggtggc atcggtcagt accagatcga
     7981 tacgcagatc cggcttgtcg gaaccatact tgtccatagc ctccagataa gggatccgta
     8041 caaacggagc cttggaagca tttttataaa taccgtactt ttcaaatacg ggaggaagta
     8101 cctgctctac gatggagaat acatcttcct gagaagcgaa ggccatctcc atatccagct
     8161 gataaaactc tccgggagaa cggtctgctc tcgcatcctc atcacggaag cagggagcga
     8221 tctggaaata acggtcaaaa ccggaagcca tcaggatctg cttgaactgc tggggtgcct
     8281 ggggcaaagc gtagaacttg ccgggatgat ttctggcagg aaccagataa tctctcgcac
     8341 cttccgggga agaacaggtc agaatagggg tggtgatctc catgaagttc agagcataca
     8401 ttctctgacg cagctctgcc acgatctgac ttctcatcac gatgttcttc ttcatgttcg
     8461 gatttctcag atccagataa cggtatttca gacgggcatt ctcgtctgca tccttggact
     8521 gattgatctc aaaaggaagt gcattgtaga tacacttacc taaaatctcg atcttgtcgg
     8581 gcactacttc gatctctccg gtaggcagat tggcattctt gctggaacgc tctcttacca
     8641 caccttccac ggagatggtg gactccttgt tgatgccatc catcacgttc atcatctctt
     8701 cggtctctac taccagctga gtggttccgt agaaatcacg caaaatgaca aatccgagat
     8761 tcttgctgac ttttctcatg ttctcgaacc agccgaccag tgtgactctc tcgccaacgt
     8821 tgccgatccg aagttcatta caattatggg ttctggactg aatcattgct tttgctcctt
     8881 cgtctgttgc tttttatctc tttaattctt ctttatagaa ttccacgaat tcttcgtagc
     8941 cttccttgtt cagctgttct ttctggaatt tcttattctt gttcatgcgg gctaccagta
     9001 cctgagtacc gtccttacgg gcttcctggg cctgtgcgat cacttcacac agtctttcac
     9061 cactgacgcc cttttcgatc aggtatgcta cctgcttcgg tctcgtagga actttgaatc
     9121 ctctctccat gagcagcatg atgatacgct cgaaaccgat ggaaaatcca caggcgggta
     9181 catcattgcc ggtgaacttg cctaccatct tgtcataacg tccaccaccg ccgcagctgc
     9241 cgccgaattc cggcatggcg atctcaaaaa tagttccggt gtaataggac atacctctta
     9301 ccagagtggc atcgaagaca atctcgaaat cagaagcctt ggtggctttg acgctgtcca
     9361 tgatctctct gaggctctgg gcgatctccg gctccaggaa tccttccagg gtatccgcca
     9421 gataagccac accatcctct gcactctcca gacccttgaa cagttccaga tacttgtcta
     9481 cacatgtctt atcaaaaccg ttctccaaaa gctcagcttc cacgccttcc agaccgatct
     9541 tgtccatctt gtccagagtg ataaatacgc tgtcatagga ctcttcgggg aatccgctat
     9601 aagcagccat tgccttcagg atacggcgct cattgatacg gatctggaaa ttcttgaatc
     9661 ccagcttacc caatgtggtg gtcgttgcaa ggatcagttc gatctccgcc agattcgaag
     9721 gttctcccag aatatcgata tcacactgca taaactgacg atatcttcct ctctgaggtc
     9781 tgtccgctct ccatacattg cccatctgca atgccttaaa aggagaaggc agggatgccg
     9841 cattattgga ataataacgt accagcggta ccgtcaggtc ataacgcagc ccactgtcga
     9901 ctacctcttc ctcggtggtc gcagctgcta cattcagttt ctcaccgcgc tttaagattt
     9961 tgaagatcag cttctcattg tcaccgccct gcttactgct taagttcgca atattttcca
    10021 cacagggagt ctcaatggaa gtaaaaccga atttgccgta ggtatccttg atgacgctga
    10081 tcacataatc gcggatctgc atctcctccg gcaggatatc tttcatgccc gttgtgggct
    10141 tcttgctaag tgccatggtt ttctaccctt ccgctcgtat attttatatc tgcaaattgc
    10201 ctttcccgca attttagctc aatatcttat atatgatact ttttttcccg ataactgtca
    10261 accctcccgg gccccgcttc tgccagaaat tcaagcattt ttctatagaa aaacaggaca
    10321 gtgatctctc actgtcccgc cttctctttc atcttcttca aataggcatc tttcaatgca
    10381 catttttcct ctaactgacg gatctgcttc ttcagatcct ccagatattc cgcagtgact
    10441 ttcacgcctc cgcggaccgc tacatcttca tgtctgctca tatcctccag ccattgtctg
    10501 atggagtttt cccgcagatc attatacgta ttgtcttcct gtggctgcca cataatttcc
    10561 ctccaaactt actcagacac ggaactgccg catattctgc tgaagtttgc cgatgctctg
    10621 taatacgccc ttggtctctg tagacatatt ttcactggaa taggtgatcn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgtctgcatc gtaatttacc ttttgatcgt
    10801 tttccccata gtgcaccatc ccttttatcc ttattttgtt ttgcgtaccg tagagtactt
    10861 ttgtcttaat tgtacttttt ctcatacaat atgtcaagaa atcaccgcgt caaatcgcca
    10921 aagtttttag ttgcagaaaa gctgctaccc acgatcggat aacagctttc tgactgcctg
    10981 ttatgatttt cagactattt tttaatattt gatctctttg ccttccagtc tccactgtct
    11041 gaactctgcg gatgcggaca gtaacaggtc ggaggaagaa ttcagtgcgg tctccacaga
    11101 atcctggatg acaccgatga tgaagccgac acctacgatc tgcatggcaa tgtcgttgga
    11161 aattccaaac aacgaacatg ccatagggat cagtaacagg gatcctcctg ccacgccgga
    11221 tgcaccgcag gctgccaggg ttgccagaat actcagcacg ataccgctta agaagtctac
    11281 gtgaatcccc agagtatttg ccgctgccag tgtcattacg gtaatggtga tggctgcacc
    11341 gtccatgtta atggtagctc ccagcgggat ggttacggaa taggtatcct tatccattcc
    11401 ccactcttca caggccttca tattcacagg aatattggct gcggagcttc tggtgaagaa
    11461 cgcagtaatg gcactcttct tcaggcactt gaagatcagc gggtacgggt tcttgcggat
    11521 acaccagaac acgatgatcg cgttggtcac aaagtagata aacagcatgg aacctaccag
    11581 tactaacaga agttttccgt attcggagaa tatctccagt ccgttggaag aaatggagct
    11641 gaataccaga cctaagattc cgaagggagc catactgatg atccagctga ctacctggga
    11701 cagaccgttg gaaatgtcat ccagtacttt cttggtggta tcattggctg ctctcatacc
    11761 gacaccgatg atcacacccc aggtcagaac accaatatag ttggcattta acagagagga
    11821 taccggattg gctaccatat tcattaacag agtcttcagt acttccacga taccgtccgg
    11881 cgctgccata tcggtggctg cgtctgccag tgtcagtgtc acggggaata accggctggc
    11941 gatcacggct accagcgctg ccagaaaggt actgaacatg tacataataa taacggtctt
    12001 aatgatgccg ccatggctct tccctgcatg acacaaggca ctgatcagca ggaagaatac
    12061 caacaaaggc gcaatggcct tcagggcacc tacaaaaaca tctcccagaa tggcaatacc
    12121 ggttgcctgc ggcacgacca gtcccaggat cacaccaatg accagaccac atacgatacg
    12181 caggatcagg ctgatgctgt tccacttttc aaatagtttt ttcatactta cactcttctt
    12241 tccctttttt ctgatgtata catgtactcc attgcacaca ttatgtgcta ttatatagta
    12301 tttctcatat aaagacaagt gtattttacc cacggacatt ttgaggctat ttgtttttcg
    12361 atttttagaa cagaaaaaaa cggatccgca aaaagcagat ccgctttcct cattttccta
    12421 ttacattaca aaacatttgt cattgcggag attcccacct tcatgacacc cgccagtacc
    12481 atgacccaga tgggattcag tttcgttttc cgaagcagca acacacagat tccgaagatc
    12541 gccaccatgc tccactccgt acccgtcagg gaaatcgtcc ctccgcttcc ccagaacgca
    12601 tttttcagga tcaggatccc tgccgatgcg atcattgcca ccactgcagg ccgcagagat
    12661 ttcagcactc cctgcagcag atccagatta cgatacttca ggtatagtct tgccaggatc
    12721 gtcacaataa tgcaggacgg caaaatacac ccgaccgttg ccaccacagc tccgggaatc
    12781 ccggcaatct tcagtcccac gaaggtggcg gaattcactg caatggggcc cggtgttatc
    12841 tgtgatatcg tgatcagatc cgtgaattcc gacatggtca gccatccgtg agtcgtgact
    12901 acttgtccct ggatcagagg catggccgca taaccgccgc cgaagctgaa cattcctatt
    12961 tgcagaaaac ttaaaaataa ttgcagatag atcattttcc agacctcctt ttggaaagaa
    13021 gtgtccggac caaaccaatc agtccgcaga cgatcacaat gtagaccaca ttgatctcca
    13081 gaatacatgc tgccaaaaaa gctcccagca taatgacaag tgacatagga tctttttctt
    13141 ttacgatagg tgcacccata tcatacgtca cggaagcaat caccgctccc actcctgcct
    13201 gcatcccaga cagcatctgc gccacgaaga agttactgcg gaacacattg tagcagacag
    13261 atattgccgc tataatcaca aagggaggca ggatcgttcc aaggatcgcc accagtgccc
    13321 ccgggatccc ggccagcttg taccccacca cgatggctcc gttgactgcg atcgcaccgg
    13381 gcgaggactg ggcaatggcc accagatcca gcatttcgtc ttctccgatc caatggtatt
    13441 catccacgaa tttcttcttc atcagcgtaa cgatgacata accgcccccg aacgtgaatg
    13501 cactcagata cagtgtggac aggaatattt tccacaatat ttttccctgg gattcttttt
    13561 cttccctact ttccatgcta cttttctcca ttctttttga cagtgtcttc ccgcatcctt
    13621 gttgttctgt ctgtcaactg taaaactgca ccgcattttt caatgctcca cacacacaac
    13681 agtatctggg cgtaaccgaa aaacaccgcc ggagtcccga tcagcgttac cagccccagt
    13741 gcccagcttc ccattctctg tacaaaatcc cacgggaaat accgcagaga cattacagtg
    13801 agcaatgccg ccgctcccag ccagatatga tcatttttgg cgcatatctt ttttcctttt
    13861 acaaagcttt gatagagcaa aaacaacaga cagccaagca acagaattcc cattccgggg
    13921 tgtccgttcc tataaaaata agtactgagc agaccgccga tactgtatcc ctgctccatg
    13981 atgctggtat attccaatcc gctctccgca aaggccccgt cgaacagcca gtgtttccac
    14041 gtcatatagg ttgggtatgc catggcaact cccagaattc cgatcaccgg atattgccat
    14101 tgtctccggc agataccacc cacgcagatc aggaacaaaa gcgtcaacgc ggctacgcca
    14161 tccagtcttc cgataatccc cagggccagt actgcggtca atccgtagcc tgtcttccag
    14221 aacattttct ccgtatccga catttttatc agagacgctg tcaggatcgg caccaccatc
    14281 catatcagaa tctgtgaccg atccacactt tccgttacca catatacatg ataaggcagc
    14341 aacagatacc ccaatgcccc caccagtgaa aaccagtctt tcttttttct ctttaaaacg
    14401 caatggaaca tactccatgc ggttccaaag gtaataatct gggtcaatat gataaaagta
    14461 ctttccatct gttattctcc tgtcatgaaa ggttcttcat tgtaccagta ctgaacatcc
    14521 gattgcagta taatggtatt ttcataatat accacctgta aaacaagtat taacagtacc
    14581 gcaaacacga tccaagggga aattttgttc tggctttttc ttttttgaag aagtatccat
    14641 accacactgc ccagtactgc atatcccaac acacaggcaa caaactttcc gggatttccc
    14701 attacatata aagtcttcac cgccagagga ttccatatac caaaagctgc tgccgccaac
    14761 accagtcgcg catcctttcc ccatttggaa aacaggaaat aaagcaatgc aagtgcagct
    14821 ccggataaaa atgctatcat gatcttatat gatgttgcga ccggaaatcc aatccaccgc
    14881 attcctgccg gaatccataa atatagtaaa tgggctgccg gaatccttcc ccattctccc
    14941 tcaagcatat actctgtttc tttcaggcac tgtacactgt cagtccccag aacaagataa
    15001 tccaccagca aggggataca ggctatcatc cagattccca ccattgcaca gttgatccat
    15061 tttttctgtc cgctcacttc tttacacaat acttttctgc ggaatatcgc aattccatcc
    15121 agacaggcaa aaagaaccag cactattaca aaaaggatcc ttcttccggc ggttgtcttc
    15181 tctactttca actcatagga aacatttcct ccctccgaaa atggctgtat ctgcagatgt
    15241 gctgcatcaa ccgtatctgc cacataatag gtaatccttt tatgctctgt atctgcagac
    15301 aaagttgccc ggttgcccct gatcgcacga aaagtggact cctccgcttc cagactgatc
    15361 tccattccgc ttccgattcc gccatttgca atatccgact ctactatgat gcgatatact
    15421 cccgggttca atgcaaaatc tttgctgacg atcccccctg tttcttccac ccgatacaat
    15481 tgctgatgat ctgcctcgaa tacaatatcc gatcccccaa aagacagcac gaacagaacc
    15541 gctaagagaa gcccttccgc taataaaatc catttctttt tcatctctgt ctcctcttcc
    15601 gaatcccgaa aaacacaata cccgctactg tgatcaaagt aaccagttcc gccgttctcc
    15661 aataccacgg ttccacgaat cgcacactga ctgttccggc atatccacca ggaatgcaca
    15721 cttgcaaaat atgtccaccg tcgtctgtga tctccaatac ttctcctttg tcatccctgg
    15781 ctgcatatcc cttatacatg agaaccggaa gtgttactat ttcctcccgg gccgaatcat
    15841 tcctgcagaa aaagcttata tttaaacctc tcttttcata gtctgcgatc tgaatattct
    15901 cattggcttc cggtcttgca aaagtcagct ttgtgctgtc cgttccatag atcagatact
    15961 cttctccgga gatgtaacca aatcccatac tttcctgatt atataatgta acgtccctct
    16021 cctgtactcc cgaatccatc ataaacagat aggatgcaga caaagacact actgccgtga
    16081 tcagactcat ctgatagaag attttccggt tgttctgaaa atatcgaatc acatatcccg
    16141 ttaccgtcac cagcagaacc gtaccccatc ccagaaaacg attggggaac tgcagactgc
    16201 tcaccagtgt cgctgcaatg ccggaaattt tctgaatgtt atcccaggga aaggaatttg
    16261 tggacatcca taatgcaagg catccgaatc ctgctgcctt tatcgcaaag ctcttctcag
    16321 cctctttttg tttttgcaaa tctcccaaaa ataacagcag ccagaaaatc atcattccta
    16381 ttaccagaaa caatcctacc ccaataggat tcagtaaatc ccctgccttg ccttccatgg
    16441 atttcggaat tctgctgaac cagaactgct gtaatatttg cgcaaaaatg gttcctctgt
    16501 cctggatagt tctcgccgat gcatggcgta tcctgacatc ctgtgtcaaa taatagtcaa
    16561 caaacggtac cagataccac aggcttaatc ccagcgcaaa aaatgctcca ctcgcaagct
    16621 gccggaatct ttgccatgca aatactctgc ggatataaat cagacagaac agcactgtga
    16681 acaatgccgt aatctcacag gtcagtacat gggtctggat caatccggca tatcccagtc
    16741 ccaaaatgat ccagctttta tgaaactctc tgtcttccac atctttttca aagacaagat
    16801 aaattccgta taccaccagc ggcagaaacg tataggcgct tccctctcct acagctccta
    16861 ccattaccag tttaaaaaat ctaaatatgg ataaagtata caatgcgctg cagaccagac
    16921 ctatgatccg gtctttgaat attccgctaa agcagtacca ggctatcgct gccgtagcaa
    16981 tattaagtcc tatgcaatag aaagcatagc tttctgtcat cgtaaagcct gccatgcgca
    17041 gaagtgccgg aaaatacaat aacagattac aatagaaaat accattggcg tatccgtggt
    17101 caaataccca tctgggttcc agccgtaccg ggaactgtcc ggtcagaagt ccatccttta
    17161 caccttcaat acggtgcaaa tgataggtca gatccgctcc ggacaccatg ccgtcataaa
    17221 aataggggat ggaggcaaaa aaggcgatca caccgattcc aaacaggatc tgacgtctct
    17281 gctcatttct gccttttacc gcctcccgac gctccagcca catggtcgca agaaccaaca
    17341 gtgctgctgc cgcaaggatc acaagatatc ttgtccacag aagattcgtc tccacgatcc
    17401 gcagattccc ggtggtcagc ttttcctgtc cggaataatc gattgtcacc gtcagttctt
    17461 ctgtgggctc ataaagccag aaatcatagg aagtataccc cagtgcactg tacagatgct
    17521 cgccgttgca caacaggcct ccctgataca cagtgccgtc tttcacattg cagaccgcga
    17581 tggcatcccc ggtgcattcg taggacagct ccacccggta tactcctgtc cccagcgaaa
    17641 tatgctcaaa taccggctct tctgtataag attccccttc cgcaaaagta tagcttccgg
    17701 tatagttcca ttcccggttg ggcatggcaa tacggatgat acaacccacc atcaatgtag
    17761 ccagaatccc cagaatcaca tatctgatat aaggtttttt cttcaccttt cccattctcc
    17821 tttggtcaca tattgtaaat catttgaaat aaaacgcaaa aatccataat gagtatagca
    17881 caaaaaaaag ataatgaaaa ctctcttcat tatctttttc ctgtatttat gcgatcccta
    17941 tgcctgtttt gtatgatgtt ccacatgttc gaaggtgaat acattcagga actgttttgc
    18001 tctctctgtc tccggatgtt cgaaaaattt atcgggagcg gcctcctcca cgatcttacc
    18061 ctggtcgatg aaaataactt tgtccgctac cgcctttgca aactgcatct catgggtgac
    18121 aatgagcatg gtcttgccct gttttgccag atccagcata acatccagca cttctcttac
    18181 catctccgga tccagtgccg cggtcacttc atcgaacagc aggatctccg gatgcatgca
    18241 caatgcccgg acgatggcta cacgctgctt ctgtccaccg gacaactgtc tgggataact
    18301 ctttgcctta tctgccagtc ccacgcgcgc cagaagctcc atggcctcct tctgtgcctc
    18361 tgccttatcg cgcttctgta ccttgaccgg tgccagcgta atattgtcca gtaccgtcag
    18421 atgcgggaac agttcatagc tctggaatac cataccgatc ttctgacgca gggtggtgat
    18481 atttttactg cccttgcgga tatccatgcc ctgcagcttt atcgtaccgc cctgaatagg
    18541 ttccagtgca ttaatacacc gaagcagcgt actcttacca cagccgctgg gacctaccac
    18601 taccagaaca tctccctgtt ttacttccag attgatgccg tccaggatct tctggccttc
    18661 atattctttt gtcagatttt ctatcttcaa caatgtctct gccatgtttt cactccattc
    18721 tcattccgac tccggttcca tttctaattc cactttttct ccagcactct ggccagaaca
    18781 ctgaagggcc agcaggcgaa gaaatacaga acaaatacca caaggaatac tccgaacgca
    18841 gcattgggac tgctcatacg attggcctca atgatctgct gtgccacctt cagcatttcc
    18901 accacgccga tcatgagcac caggctggtg gtcttgatca tacgggtgat caggttgatg
    18961 gacagcggaa tcagtctccg cacagtctgc ggaataatga tatacagata cgtctgtatt
    19021 ttactgaatc ccagtgcttc actgctttcg tactgatgct tcggaatact gatcagcgct
    19081 ccacgcacca gatctcccat ctctgcggtg ccccacagga cgaagacgat gacggatgcc
    19141 acctctccgg acagattcca gcccagtgct ctggtagatc cgaaatatac gataaataaa
    19201 agcaccagct gcggcatgat acggatgaat tccagataga cccggaagat agctttgacg
    19261 aaccggttct tcaccgtcat caacgctccc accagaatac ctaacggcat actgatcaga
    19321 atagatacga tactgatctt catggctacc cataatccct gcagcagacg gagcatgttc
    19381 gtgcctttga ataatacatc aatccccaaa tccggcataa cgcatcctcc tctccaccag
    19441 agatcccacg atcgataccg gcagcaggat caacagataa aataccacca gaaggaaaag
    19501 gctctcggta gtcttataat acaggccgat cagatccttt gccgtaaaca tcagatccat
    19561 cagactgatg gcactgaata cactggtctc cttcaacagg aaaataatat tcgccacaaa
    19621 tgccgggata ctggtagaca tggcctgtgg caggatcaca tatcttagta cctgtccgga
    19681 gttcattccc aggctgatgg cactttcatt ctggatcggt tccacggcct ccagaccgct
    19741 gcggaatgcc tccgccatat aactgccacc taaaaatgcc agtcctacca caccgcagac
    19801 ctctgctgac atccggatcc caatcttcgg aaatccgtaa taaataaaaa acagctgtac
    19861 cagaagcggt gtattacggc tcagttccac gtagaccgct acgatccacc gaagcaccgg
    19921 tactttataa tattgtatcg ccgcacatac cagtcccagc agaattgcaa acaggattcc
    19981 gatgacaccg atcttcacgg tgagcaacgc tgccttttca tacataggca gatattttcc
    20041 tataaattcc cagttcatgt atttctttcc tttcaggaag cttttcaaca atcgtaacta
    20101 ttcagaatag ttacacacaa ttttttagat tataacaaaa gaagtagcca gatgcaagaa
    20161 aagactgcgg cggcaacata cg
//
LOCUS       DAWEJW010000051        20099 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1048_length_20099_cov_64.489373, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000051 DAWEJW010000000
VERSION     DAWEJW010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 20099)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20099)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20099
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1048_length_20099_cov_64.489373"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3346..3445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10727..10826
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4607 a         5540 c         4456 g         5296 t
ORIGIN      
        1 acccacacat agccattctc gggctgcaga tcggtataca tgagcagttc cacataattc
       61 agcggtcgga acaccggatt tttcacggaa tcatccttca gcaccgcgat ccgctcttcc
      121 cagccggcgc cgtaggcctg tgcccagccg tttctccaca tgcggtaggc acggtaactg
      181 gtggtgctct ggacattgcc gaacagaacg actgctgcca gaagtgcccc cgtcactgca
      241 aaataacgca ggaggtattt ctgtgccaac aggtctgcct tttcactctt ttccgctttc
      301 agaacccgct ttgcgatcca gccacaccag tacagtacat tggccaccat ccacaacaca
      361 taaaaatacc acaggatagc tccctgcctg ccgccgccca tggtgccatc cacatagatc
      421 gttgcagtag cctgggaggc atataccccg aaggtcagcg cagtaaacag gccggggaag
      481 cgaaactcat agttcatttt tctgaccgct ttccagaaga aagggatcag cagaaccagt
      541 aacagcagga ccttcagatt cgtccagctg ataatattca gaaaagttct ctccagggac
      601 agccagatgg ctatcaaagg ggaattggct gtgttgccgc caaaattgcc gttgagtctg
      661 gtggctgtca gcggagaagt cacacacatc aatacgctga gtgtctctaa cagtgtcacg
      721 atccaggtcc ggcgaaaagc atctttacgg cagatcagga ataagatctg cagacagatc
      781 aacaggcaca tggtggatac gctggtggaa aaattgttgc cacccaccag aatcgccagc
      841 aggcaggcca gcacggtaag cacgacccgc tttgccttgc cggtctcgcg aagcgcaatt
      901 tccagataca gtgtcagcag gaccagtgac aagctataca caaaagtgta attcaccgaa
      961 ccgttatacc aataaaagct ttcctccgga taggggcagt aaagcacctg gagaattgcc
     1021 atgggaatac cgatgggcag gatcagccag cggttctcct ttgcctggcc cgcaatgcat
     1081 tctctgagca gcaggaactg ggacaggatc acggaaccga tcatcagata gggggtgatc
     1141 cggtaaaaat gttctccaaa tgccgcaggc tgcaaagccg acagaatcag tatggaaaat
     1201 ctggcttccc acacattttg tgccaatgtc tggtatatcg catgaaatac accggcgatt
     1261 ccttctccgg cctgcattgc ccggtacata tattttccga aagaccagtc atctgcagag
     1321 ggataatcgt attttgcgat cgtaaacagc ggccacagga tcaccgccag tgccaggatc
     1381 cacgcgatgt ggatcaatat cattttcttt cgttctgtca gtttcattgg tataaaatat
     1441 cctttcttac aatagatccg gtgtccagtc catatctaca tcacttccgc cgctgtctcc
     1501 accggaactg ccgctgtcac tgcttccgct atcgttgctg ccagaatcac tactgccgga
     1561 atcactgctg ccgctgtcat catcgtcgtc atcgtcatca tcctgtgcag gttccggggt
     1621 cggagccggt gtgggcgcag gggtcggagt gactgcaggc tttgtcgcag caggtttggt
     1681 cgttggcttt ggcgtggtgc cgggattctg ggtggctgca ggtttctgaa tacttccgcc
     1741 ggtcttgtct cccgacacgg ttccgctgcc ggtgatcgtc tgcccatcgt taccggctgt
     1801 actgttgcca tctgcactgc cggtgatttt gccggtaatg cttcccgtga cgcttccgga
     1861 tgcagtggta tcatggtttt cttcataagc cgcaaaagga tcctgttttg ccatttctct
     1921 gaccgcaccg gcgctgaccc agtattttcc gtcaaaccat tgtacttctc cgtcaaaaat
     1981 acggatctgc ggggtatcac tgtctatggc acccgtggtg ttgtccgctg cgttgccgtt
     2041 ctctccgccg tccgtggcgg caccggttcc ctctgctgta tttgcattgt cctctgctgt
     2101 catatcctct gtcacctcat aggacgtgta ttccggacgg gtctccagtc cgatcatttc
     2161 cccgccaaaa cgatactcct ctgcggtaat atccccgccg attccctgcc acaggcagtt
     2221 ctcaccggta gcatcatagg catagaggat cagatccgta taatctgttc cctccagcag
     2281 ttttttcttg atttcttcgg aaggctgctc tgtcaggatc tttccttcgg aatcaaaact
     2341 tcccgtgtaa gtgatatatt ccatgccctc ccggttgctc cagagggaat agacatccat
     2401 cgcaccttct ccggtatgga catcacaggt atagtctccc gtgagcacac tgccctgcat
     2461 ggttccctgt tcatgggtaa tgctcccggt agcgctgtcc acgctccagg cttcgaaggt
     2521 accgttccag tccttcatgg tttccggtgt atctgccaga ggcgctgttg tatccgaaat
     2581 ctctgtcatt gtattttccc gggctctggc ttctgtggtt cccgtttcct gtgtggcatc
     2641 gggattctca gtttctgccg gatccgcgat gttctcgtcc gcaccggccg ctgtcactgt
     2701 taccagccgg atcgttgcac cctcctggct caggaatcgt ttcgtgtcat ttccggtata
     2761 ccacaccctg gagaagcatt gtccctcttc cgtatagccg gtctgtacat agagcagagg
     2821 ctgactgtct ctttccagat agtagtttcg ctgtccttct ttcagtttgg gcatcatggt
     2881 ggtgaaccag tcctcgctgt cgatgagatt cacgctctca ctcagatact cggtcagctc
     2941 cagatcattt tgcatttcct gtgcacggct gcggatcccc tctgtctctt cctccagatt
     3001 cacgctgagc tcctgtagag cagcaaatgc ttcctcatcc gcatagagcc ggtaatcctg
     3061 ttccagcata tcccgctgtt tgcggatgta tcccgctctg ttgtaggcat cggccaatgt
     3121 cagataatcc tcctgggtaa actctccttt attatatttt gtctccagat tgagaatctc
     3181 cagctccagc tctgagagca tcggctgtgc ctctacggga gtcacggcat ccttcgcatt
     3241 ttcatcactg ccatctccgc aggccgtcag ggatgttacc agaacagcgc tcatggccag
     3301 tgccagaaat cttgttgctt ttcttttcat ctttttgtcc tatctnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnntagat gctgcgggcc tgcaccggac cgtagatggt
     3481 aatgtccttg ccatccttgt tcaggatgat gtatccgcgg aatgcatatt ccaccttata
     3541 ctggtttgcc ggaaggccga ccagtacgga agtgaaccgg tatctgccac tcacggtctc
     3601 ataaatactg tcctgatagg tgccgttggc atttgtaccg taagccagtc cactgatgac
     3661 tttttcgccg cctttgatca tgggatagct tgtccggttg gcattgttca tgaccagtgt
     3721 tccgtattcc ttcagatgat agccgttcac accatttccc agcagttgtg ctctgaggtc
     3781 cgtactgata ccggtcttaa agcggattcc cgcttttccg gtaatccgga tggaaaatcc
     3841 atggtaggtc agcagatccg taagtcccgg ctgttctgtg gccacatagg cattgttacg
     3901 atattccagg gtccagacat acatgccgat gggcgctccg ttctcattat accggtacac
     3961 gaccgccgac tgggcatttc cgttcggcag tttgacgata cggcggttat tccgcacctg
     4021 tggtgtatag gcaataccgt ccacgtagat cgtggaagca tcgtaacccg acggtacctg
     4081 caataccaca ttggtagagg aagtcggcat cgggcagggg ctggccggca tattttcata
     4141 gaccggaatc ttgaagacaa aggtattctc cagcgctccc gcactttcat acagtttctt
     4201 catgctcagt gcctcactgg tgggtgctga gatgttctgc atatactgat gggtatatac
     4261 cggattgctg gcagtggggg atacattgaa tttctgtaaa tacagggtat cctggccctt
     4321 acgtatgtag cttgcggaga ttacttccgc tcctccaagg atcgagtagt aggctccatg
     4381 ccagtcatga tctttggcat aattcaatcc attgcggatc acttcttcat tggtactgcc
     4441 ggaagctccc acattgaaat aattgtaata atgttcataa ccgggatagg tgccggagat
     4501 cagcggggat gtcccctctc cctgctcctg cagtactctg gctgccagat ggaacgggct
     4561 gacctgccgg ttctcctccg caccgatgga ccagaaaata tggtagaatt tcattgaggt
     4621 ctccggtgcg ttctgactgc tgttcatgaa ggttccctcg aggaaattct ttaccgcttc
     4681 ttctgtatga tagcttgcgt tgtaggtcag ctgttcaaac tggaaaatag cattctcctg
     4741 cagggaattt ctggggtcca tgtataattt cagtacatct tccgaagcga aataccagtt
     4801 ggggctgtgc tgtccttcct tgcaataatc cccgtaactc ttatagacca gactttttcc
     4861 gccctgcagt tcgttgttga ctgcggtctg gaaatccagt ccggtattct gtcgtacaaa
     4921 ggtccagttg ggatgtttct gtttcaaagc ctgcaatgcc ggccggtagc tctccgggaa
     4981 ctgctcgatg tccgcataga caccggtggc attttcctct gccagcataa cctccgctcc
     5041 gggattcatg ccgtacagtt cttcccattc cagaaaacgt tcatccgagc atgccagata
     5101 atcccggggg atatatccct cgtagacctt gccctttacc tcggcggata ctttttccca
     5161 gacctgatag tcttcatcca tcgtcacatc ccggatctcc accatctgtc cactgggcac
     5221 ggtgaccact tttgtgctgt cttccgccgc ttccgcacgg atcgtcagat catcgcacag
     5281 atagaccagt gccatgacct cccggtcttc tacgatattc tgcaaagccc ctcgtgcttc
     5341 ttcgatccat gcctgatctt cttcatccaa tgcatgcgtc tgataggtat tgccctcgta
     5401 ggtgaatata aatgccgcca ggagaattgc tcccagaaat gccaattttc tcttcatata
     5461 acgtgactca ttccttattt tatggttgct gtgttgcccg ttctatcctg cctctacgtc
     5521 gggcatcact ctatgtgctt cagtttctta tgcagtaccc ggattccctt cggtgcaatg
     5581 gcaaacagtg tatgataaca cttgttcttt ttggtaagcc agagattttt catggctctc
     5641 caccagccgt gacgcaagtc acgcaccacc ctgcggtaga atgcgttgtc cccggtcatc
     5701 tgggcgatag ggatgtgcag cagataatcc agccgctgga agattccaaa ccggaatgcg
     5761 atttccgaca gctccggata ttttttatcc accagtgcaa gcaccatgtc cgcattgtcg
     5821 atattgtcgg cgaatactct ggagaaccgg ttcttgtctc tggtattgct gtccggacga
     5881 taaaatacat gatacgcctg tccgggaatg catgcaatcc ccggaactgc actttcctcc
     5941 cgggtcagca tttttactaa taaatgaaaa tcttcgttca gtgcccccac cggaaattgt
     6001 tctatggtaa acaggtcctt ccgcaaaagc ttggtacaga aggagcagtc tccgcggtgc
     6061 atcagaagtt ccttcagaaa accttctgcc gggatcacct gttccgttgc caccggctcg
     6121 cagatattcg gtaaaatatt gccgtctttg tcgatttcat ctctgccaac ctgcgctacg
     6181 ggcattccgg tcttttcgat tgccccgtac agccgctcat acatatccgc atccacgtag
     6241 tcatcgctgt ccacaaagcc cacatattct cccccggcct gctgtaatcc gaaattccgg
     6301 gcggaagaag gcccgccgtt ctttttatgg aagacgcgga ttctctcatc ctccgttgcc
     6361 agccggtcac ataatgccgg tgtctcatcc gtggaaccat catcgatcag caggatctcc
     6421 agattttcgt aagtctgtgt ccgcaggctt tccacgcagc ggggcagata cggtatgata
     6481 ttgtacaccg gcacgatcac gctgatcagc ggttgcttgg ttacactact gttcactgtt
     6541 tattctccta tacatagacc gctccgggat cacaggggcc ggagagcagg ggccgtcatt
     6601 ccataattgt ggaatcaccg gggcaggtgt tgtgatgttc agcatcacat cctggccggc
     6661 atccggcatt tcctgttctt ctgacatttc tggcaagaac ttcattttcc ggcacagttc
     6721 gatcaatctt tccgcagccg tccgggcatt ccattccccg acaatggtct gctgtgcgtt
     6781 ttctcccagt ctgcggcact ctccgggatc cctgagaagc cgttccacgg tccgaaataa
     6841 ttcctgttcc tcaccgtccc ggtacatcct gccgttttct ccctgacgga tcagccaggg
     6901 agccgcgccg atcatatgat ctgccaccac agcacagccg ctgttcatgg cttcattcac
     6961 cactgccccc cagccttctt tgcggtcact ggtgaccaga tagatttctg acttttccat
     7021 ggccgcccgg acttcttccg ggccttgaaa accgggaaag gtgatcacgt cggtcactcc
     7081 gtgttcctct gccaggcgat gcgtctcttc ctccagttcc cctccgccga tcatggtaat
     7141 atgaaacgta ttttcttcca gatgttcttt taaataagat gctgttttca ccaccagttc
     7201 cggatgcttc cagtcgatca tccgggccgc ccacagaata ctgcccggtt ctttgtgatt
     7261 gaagggctgc cctgcctcgt aatgcctggt ctccgggaaa tagccgaagc gcagcatttt
     7321 gccggggtag gcatggatca ggtgataatc gcaaggcaca taggctcctg cgcacaggaa
     7381 atacaccggt gccttgcggt atctggtgtg atccttgtat ttctgcagca ggcctctcgg
     7441 agagaccgcc ttccactgtc cttccttata taagcgttca ttataacgga agatcggttt
     7501 tccggcagtc agtctctcct gaatatagct ttcgtcatcg catccgccga agatcaccac
     7561 atccgcggta aacaatagtt tctcaaaatc ttccgcatct gtgacggcgt tgctgttcga
     7621 ctcagagccg ggcgttttct ccctcccaga cttatgtgca cacgaattct tcgcagcgta
     7681 gcgcacgtag ggacgttcct cggcctgcca gcccatcttc acccgctctt cttccatggg
     7741 ctgtgtctgc aggaaacaga aatctccctc cagcaattca tacagagcat cacagaaggg
     7801 cagctgatga tgattaaaat agtttgagac aaatatcagc ttcattcctt cctccctgct
     7861 atgtttgcat agatttcctg cagttttgcg aaattttgct ctttattatg ggtttttctt
     7921 gcatgttctc ttgcattttt tgagtattcc agcattttct ccggagaact ccacatttcg
     7981 ataatagaat ttgccatgga atttacaata ttttccaatt cttctgtttt tgtcgtttta
     8041 taatttcgca tgttattttt gctttcagaa gcatctgacg catatttgtc attttcagcc
     8101 acttccgaca gctgatgtcc ccggcaccac aggccgtccc ttccgccatc aaacaggctg
     8161 gggattcccc ctacctccgt gctgatgcag ggaactccca gaagcatggc ctctcccagg
     8221 gaattcggtg aattttccag agaggaacaa cacaggaaca gatgactttt cagaaatctt
     8281 tccttcattt cttctccggt cagttttccg aggaagtgta ccctgtcctc cagctgtcct
     8341 tcccggatca gtctgcgcag gtaacggcca tatgccgata ttttcagctt ttccttcggc
     8401 gtccggtatg ccgtcagatc atttcctgcc acatacacct gtacccgggg atatttctcc
     8461 cggatctgtg gcaaggcctg cagcagatag tgcagtcctt tcagaggata atccgcctga
     8521 ctgacgaaaa tggaataggg ttcacagtgc tccggctgcc attgtccttc ataaaaggaa
     8581 gcccgcaggg tctcattcat cgtataataa tgtgccttgg gattccacgc agtagtcact
     8641 gccctgtccc aggcggttct tccggtgata ttgccggcaa gtcctattgc ttccctctcc
     8701 attacacctc tgcgggcaaa tttttcctgc tggctgcgca gggtatcctt cttcaccaga
     8761 tcccggaaag tggtcttgtg gatctccgat tccggcatat cggcaaaata tgcctccgca
     8821 cacaacgtac agattccctg aattcccacc agtatcttct ccttgtgggg aaacacccgg
     8881 catactgcca gtgtgtgtgg atattccgtg ccgaaacagt ggatcacatc cggtgaaaag
     8941 tcttccgtga tccgatgcag ttcttcttcc agcaacggct gatatctgtc ggggtgtacg
     9001 gtgtcttcgt ggaatccata acatgtgata ttatatgctt cagcatctgt atttgtctgt
     9061 aaatgctccc ggtcttcttt ttcccgtggc attgctatct gcattctcca gggcacctcc
     9121 gtatctgccg gcaccgggaa tgccacagcc agctccaggt cactggaatt ctccgcggct
     9181 acctgctcca acagaccgga cagccagccc tctttattcg aagcttcttt atgcaatgct
     9241 tcggctataa ccggaagcat gatattgcag acccacagca ctttcataaa ttaaatcctc
     9301 ttttaattaa agatccagtt tagatacgga agcagccgta tctccacatc gtacattccc
     9361 cgcaacagca ttacgaaata caatagatat gctgcaacaa cgccaataat acacagggtt
     9421 cggaaccatc cctttttggc atccttcagc acacccggaa tcaggaaaat ctggctttgg
     9481 atcaggtaat atgccactcg ggatacttcc gggataaagg agccgcagga atagagcacc
     9541 agtcccgcca gattcaggta gaagtaataa cggtttctgc gattttcctg aacgctgtcc
     9601 ttccaataga tcaggcacag tatcagcact gccaggcatt tggcgatgtt gacataggat
     9661 aactgtccgt tgtcgaacat ggaatccttg taatagggat agaaccggaa gataatattc
     9721 cggtacacat cctgtccgaa gatcagagat aagatcagta atacgccaac cgcatagtgc
     9781 cactttttca gccgaatccg ggacaagaga tctatcacaa tatatgccgg gatgaccagc
     9841 agtaccgatt tgtggatcgt acagcccagc agcacccaga gaagaagctt cccatattct
     9901 ccccgcagca catatttctg cgcatacatg accagtgcca gtgcgaaata gtaacgtacc
     9961 gaattcatac tattaaagta aaatccctgt gtcaataaca gaaacaggga aaatacatac
    10021 cagtccgcct gatcatgcag tgccttcagg aaaaagaaga ccgtaatcag ggaaaaaaag
    10081 ccaaagatcg gaagataaga tccctctccg aaaatcagat ggaaaaattt caccagatac
    10141 acaaaaccgg tttcgtagga gacatgcctt ccctgatcga tcagctgaaa tttcagccag
    10201 tagacccagt aatcaccgcc cacagcaatg cggcaggcag aaaccccggc caggagacag
    10261 aagatggcga attccgccac agcattgcac gcctgctgtc tgtccggccc ggccgccatc
    10321 agatccccgg aggtattccc acgaaaggag cgtctgccgg acagatattc tgtcgtatag
    10381 tcacgatttt ttaccagaaa accgaatgct acggtaacag ctgtcaatat gatatacacc
    10441 agtgcgctct tctccatgtg gaacctcctt tctttatggc cttttttcca aaaagcaagc
    10501 tatcgtcctc ctgcctcccg gatgccgtca cgcatccact gtctggcctg ctgcaggctt
    10561 acctctgtgc acagcgtacc cttatgagca tacaaaaacc gcagtgccag cggccattcc
    10621 agccatccat acagataatg atacaatact cctctgtcat ggaaaaattt ttcattatag
    10681 ccatgaaacc aggtggattc cccggcttcc tctctgccga tggtcannnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnatat tgaaaatgat catatcggcc cggggattcc
    10861 gttcaaactc cgcaagaaca gccgcctcat aaccatcttc atacacaata tctgcatccg
    10921 aaaaaaggca gatgtcccct tccgcccgaa ggatggcctc attgcggctt ctgccgatgc
    10981 cacggtcggg aaaactgcag aaacggattc ttccgttccc cggagatatc tgcatttcct
    11041 cctgccccag ccggtcgcac tgattgatga tcaccgcatc cgattgcagg ttcatatgtt
    11101 gtacggtctc cggaagagaa gcattcatta ccgatgccag aacttctacc ttcatgcctg
    11161 ctctcctccg tgttctgtat tctgtgtctg catggctccc ggcagaaaat aatagctgcg
    11221 gatcagctgt tcatccgcat tccacaggaa ggctccgtcc acagagcagt cctgggtatg
    11281 cagatactct aataccgctt ccagtctctt tcctacccgt tctccggccg gcaccgccag
    11341 cagaaatgca tctgccttac gaagtgtctc tccactctcc gggcataaca acggactggg
    11401 aaccgctaca tattccaccg gtattccctt ccggatctta cctgtcttag ccagcttctg
    11461 caaatcctcc acgacttcct tcggatccgc ttccggcagt gccacacata ctgccacacg
    11521 gcagatttcc gcattttcgg actcctttgc ctgcttttta tcgtacactt tctccagcgt
    11581 atacatcaga ttttccgcaa atcccacact gcgcaccgtt cccagactgt tcagtccgta
    11641 gcgtctgcga agtgttgccg gcagccagat gctgtcctgc gagatctcca ccagcaaaaa
    11701 gaggactact gcaaaaaaca gagacaatac cgccgccaga atgactgccc gcaccgggcg
    11761 cacatccggt accacgactt ctgcaaaatc tccgtgatcc agcactttga tccggtcgat
    11821 ctccacgata ctctctgcaa attcgtcaca catggtatcc tccactgcat gggccagagc
    11881 aatactcatc tccggatcct gagttgccgc ctgtgtcacc ggaacactga aatccgaggg
    11941 aagtgttgcc gagaggtctg cgatccactg atctctgccc agagacagtg cttccgaagc
    12001 tccattgtct ccccgttcca cagcctcctg cagatgcttc tccacaccat ccaggaattc
    12061 tcccgtatgg atcatggtgt tccatgtagc ctcgttaatg tagtacgcac ccgccgcatt
    12121 ggggttctct ttatattcta ccttataagt ggaagaagcc gcataccctg gatcgggctg
    12181 cagcaccaca ttttttacat aataaccgcc accgaacagt aatgttccca ctactgtcac
    12241 agccagtatt ttccacagat tgcggcatag ccgcagacac ataagtctca ggtcgaaggg
    12301 ctcttttccg tagcttactt ccatgcgtct acctcctgcc ttctcagtcg tcatccttct
    12361 cggcacgacc atcgtaggct gccttgaaag ccgttacctg ttcgctctcc acaccctcgg
    12421 tactgtccgg ccagaacaat accttcagtg tcagaagcat caatttaaga tccagccaca
    12481 cagaataatt ctcgatatat gttaaatcca gttttaattt atcatacgga gaagtattgt
    12541 actttccgta tacctgtgcg aaaccggcca gtcccgcttt caccttcata cggaatgcga
    12601 actccggcat gacttccatg tactgtgcaa taatctccgg gcgctccggt ctggggccga
    12661 tgaaagacat atctccccgc agaatattga tcagctgggg cagttcatcc agtcggcatt
    12721 tgcggatcac cttacccacg ggagtgatac gcttatcccc tttctgtgcc agacgtgcca
    12781 cgccgtcctt ctcggcatca gtgcgcatac tgcggaactt cataatatag aattccctct
    12841 gtccgatggt gcagcgtacc tgcttgtaca gcacaggacc gccgtcataa agcttgatgg
    12901 caagcgctgt cagaagcatc agcggtgaag tcaccaccaa caggatcaac gagcagatca
    12961 catcgataga tcgcttgatg aaccgctgct ccatactcag ggagtactct ctggtcagca
    13021 ggatcggcgt atcaaataca tgcagttcct cggagccaac taaaataaca tctgatattt
    13081 taggcatgac atagacacgg atggaatggg catagcagaa tttcaggata atattacgat
    13141 ccttttcatt aatatcccag atcactaccg cattgatctc accgttccgg taattgtcca
    13201 gaatctcccg gcacagttct gtcgtacctt ttttaatgtg ctcacatttt gtgatcttat
    13261 atttgtcctt acggctttca aatttgttga caatgtattc gatgggacga tcgccgtgga
    13321 tcagcagaag ctctctgggc gggaatgctg tacgatagat cttgttggca atattggcat
    13381 agatgatcac cgctatgatc tgtaaaaata ccatcaacag gaacatatcc gccggaaaaa
    13441 tgctacgggc catcatacac agttccgcat agatcagcac atccgccatc agggttgcaa
    13501 acacctggga gaaaataatc tccgtgtttt tcatgtatcc cagacgcatg ccgccgtagg
    13561 tcacggagaa aaatatcagg attgcactgt agataccgtt ttccagcaga aagccacgat
    13621 accagatctt ctgcagggga tctaccagct ggaactggaa atggaactgc cacatgtagg
    13681 caaacaggct gatctccagc aacagacaga tcatcgacaa ccctatattg atcagacgct
    13741 taaaggattc ccttttccgc agtcggtttt cataataatt accgtttgta ttcaaaacaa
    13801 actcctccta tactctgcgt tttacggcgc gtaccgccca gaagcagaaa tgccaggcac
    13861 tgttcggcac tgacatgcct tccgccttac ggtacagatt ccagatcctc cgcaaagctt
    13921 ccagcttatt cgatgacagg gatttccctg ctctccgata ttttaccaaa ttttgatcca
    13981 aaccacaagc tacataaccc tcccgcagta ttctccacca cagggcagtg tcctcactct
    14041 tgatctgggg catctgcagt tgttctttcg acagctgttc catatcgaac atcacggtac
    14101 tggtaaaaat tgtggtattt ttcaaagctt ctttataagt aatcgtggcc ggcacacgga
    14161 caatctttcc ggtgcccttg ccattctcat cggcgaattc atagccggta aacacaaagg
    14221 ctgcctgttt ctctttcaga aaagccagct ctttttccag cttgtccggt gtccacaggt
    14281 catctgcatc cagataggcc agataccggc cttttgcttc gttgactccc agattacgtg
    14341 ctctcgccgc ccccagattc gtagagtgag tcagcaggcg gattctcgta tctccggttc
    14401 tctgaatata ttgttctatg atctccctgc cgcggtccgg gccgcaatcc tccaccagca
    14461 gcagttccca gtgtggatac gtctgtgccc ggacgcagtc catcgtttct tctatataac
    14521 gttctgcccg gtacaccggc accacaatac tgatcagttc ctgttccatt ttgctttcct
    14581 gttcctaatt cttacaaatc gttatagttt tccctatctt tccgcaagat gcttacttac
    14641 atcacgatca ggaaatatcc gccaaaggac cgcagtctgc cgtccgcagc ctcttgcaat
    14701 tccttctgta catcctccga caagtcctcc ggcaataaaa tgtagtctat accggccttc
    14761 ccggcgctga caaggcacgc agcaaaatct attttcctgc caccggcttc tgtcgtggta
    14821 ttttcatctg tccgcagata ttccgtctcc ccggtagtct ccaggtggct catccacaga
    14881 tacatatctt cctgccaggt ctcataggta tcataggagt agctgcccag cgatgcatcc
    14941 cagatgcttc tgccgtatac cgggtggatc tcagcgctgt aggctctggc agcctccatg
    15001 acctccttcg gcgcccacag actgtattcc cgtgagtttc ctttttccgc attgtataca
    15061 gaaagctgct ccagaatttc cctggcggga gttccctgtt gctgtccggg cattcccgtc
    15121 agtgccggta tctgcgttgt ctgtgccacg gtccattccg acaccgggtt tccacccagg
    15181 aacaggatgc ccagcaggat cagcgcagcc atcccgtttc gcaggctttt ctcttttaat
    15241 aagctgcaca gaaagtccgt tgctgcccat gcgatcaatg ctgtctgggg taccgccgcc
    15301 cagatccata cataagagta aaaggcggtc tggtatttta acaggatcag tgccgttacg
    15361 ggacagatac agccaaatgt caccagcgct ccgtaaagat acagccgttt ccgtgctttt
    15421 tccgccccgg atgcattggc ttcctttccg gaaccataga ggacaaatcc cagatacagg
    15481 atcgccagca gcaaaaatgc cgcaaatttt ccatgttgtg tatagtcggt ccaacctgtc
    15541 agggtggact gcagaagtgt catcattctg tagcctcctc tcctgacctg ccatgtgcat
    15601 cagtcatttt ccgtttttcc catcgtttcc ggaactggga aaccaacgct ccaagtatca
    15661 gccatgccac agtaacaagc aggcagactc ccgcgccata cagggtccag accatgcagg
    15721 cctccgccag aatgcagagg atcacaccca tataccttct gtccatcagg caggacagca
    15781 gatacggaag cagcaccagc gcacggatcg tcacaccacg gaatcctccg taaaacacgt
    15841 caaatccatc tacaccgggc atataggccc ctgtactgaa cagtattccc acgatcagca
    15901 aaaacgtccg tcttttcaca gaattttgcg aaaaaagtac cttcccaagg ctttcgtaag
    15961 ctgcatagga cagcagcagt gtaatgcagg ggatcagacg atacaccacg tctgtgggtg
    16021 ccatacccgt aaaacggctg atggctccat acagagtggg caggcataat attttcagcc
    16081 ggaaggacat tcccatcgaa tatgccgtcc ccgtgagagg attcacggta taaacagcat
    16141 tttccctgag aaatgtgttc acggtctcca ccgtcatatc tccggtcagc cacatgcggt
    16201 tccccgtcag tatccgcagg atctgcagca ggatcagaaa tgccaggatg cctgtgagaa
    16261 tctgtgtgct tttttccttt gccgtgtctg tcccgctgtt ttgatccgca accggatcat
    16321 ttttaccggc tgtggcccgt tttcgtgtct gtctggatat tacagacaat aatatcgccg
    16381 ccagcagaac gattcccacc tctgccagca gcagatccgt tacagtcaaa aaagagcacc
    16441 ctaacaggca gcctgccaaa tgggcgatct ctgtcagccc gataatgatc aggctccccc
    16501 cgatcaaaat atccgatgtc cgaaaagtca tttcatctcc catacccgcc tttttcttac
    16561 acgggctttt gtacatctta ccatatttat atggacttga caaatataac agcatttcca
    16621 tacaacaaaa aaacgcgtac cggaaacttc cccattccga tacgcggtct ttctcagtct
    16681 gcagaggaga atactgcatc tgcttaatta acaaactctc cctgttccaa ataattaaaa
    16741 ttaatatgtt acatagcctt ataatccagt cggatgttgt cggcgatacg tgcgatgtac
    16801 tcgctgttcg taggtcttgt ccttccggag gctacagaat agccaaaaat cttttcgaat
    16861 gtctccacat ttcctctgga ccaggacact tcaatggcgt tcctgatcgc gcgttccact
    16921 ttctgatctg tggtcttgaa atgtttcgcg atggtgggat ataacagctt ggtaacgctg
    16981 cttactacct ccatgtcttt ccctgacaaa atgattgcat cacgcaaata gtgataaccc
    17041 ttcaaatgcg ccggcacccc tatctccaac atgattttgg ttacatatct ctctaactct
    17101 gaatctttta ctgcaacaat atccaaaaca agtccccctt tttacttgtc gagtattgct
    17161 cctctgcagt gactgataga gaaatcatat catagctgtc gatatttgaa aagacatttt
    17221 tccgttaaaa tatagttaag aaactaaaaa tgttacattt tacccctatt ttgtaacatt
    17281 tttacgtaca taaaaaacgg atatttatgc ggtttttctt aaatttcctc ttttaccagg
    17341 taaatcggac ggcgttttac ttccatgtat gcctttgcca gatactgccc cagaataccc
    17401 gtacagaaaa actgtacgcc gctggtcatc aggatgatgc acaccagaga aggccagccg
    17461 gacaccggat ctccgaagat cagcttccgc acgatgataa atatgatcat caaaaaagcg
    17521 atgatacaga acagcacacc cacaaaggaa gcgatcaaaa gcggtgccgt ggaaaatgcg
    17581 gtgataccgt caagggaata taccaggagc ttccagaaat tccacttggt ctcgcccttg
    17641 gcgcgctcta cattctcata ttccagccat ttggtcttaa aaccgaccca gccgaagatt
    17701 cccttggtaa aacgattata ttcccgcatg gacaggatgg catccaccat ctgtctggtc
    17761 atcaggcgat aatctctcgc tccgtccatc atttccgtct gggaaatctt cttcatcaga
    17821 tggtaaaatc ttctggcaaa aaagctgcgg atcggcggtt cgcccttgcg ggtcacgcgg
    17881 cgggtcgcta cagaatcata cccctgccag atccattcaa acatctccgg cagcaaggaa
    17941 ggcggatcct gcagatccac atccatcata accacgtaat cgcccttcga cttttccagt
    18001 ccggcaaaca tcgccgcttc ctttccgaaa tttctggaga aagagacaat atgcacacgg
    18061 gaatcctgtt cgatcagctt tttcacttct ctcacagtgc cgtctttgga accgtcatca
    18121 atatagagaa gctccagttc tatttccttt tccttgaaaa agggatcatt tttcgtcaat
    18181 tcctgataaa acagggcaac gctttcttct tcattatagc agggaacgat cacgctcaat
    18241 aagctcattt tgtaatctcc ttatgttttc ctgtcgcttt ttcaggctta tctgcgccac
    18301 tgtgcgacag ataacagcta tattatacta tccttcgatg catcatttca aggtcaacac
    18361 tgtacttttt ccgctttttg gattatactg gatttatata tgtctgttct gcaaactaca
    18421 gcatatagga aacgaggtta tttatgactt tttttgaaca gtttttcagc aataccattt
    18481 tcctgactgc agccttcggc tggctggtgg cacagattct gaagacgctg atccatttga
    18541 tcgtctccaa gaaattcgtt gcggagcgtc tggtcggcag cggcggtatg cccagttctc
    18601 actctgctac cgtatgtgcc ctggtaacag cggtctgcta cgaatacggt gcgggaagct
    18661 ttgaatttgc catttccctg atcctggcga tcattgtaat gtatgatgct atgggagtac
    18721 gcagagagac cggtattcag gcacaggtat tgaacgaaat gctgagggtc ttcgaagata
    18781 tgggccgcag tgagatttcc gcccatgata aattaaagga attcgtggga catactcccc
    18841 tgcaggtgtt gatgggtgcg ctgttaggca ttttatgcgg cgtggtcatt tacagcttct
    18901 tactctgata accgatcgag gtgtgattat atgtgtaaat ataaatttcg acggcagttt
    18961 ctgcagactg cggtacctct tctgctgact gtctccatga tgcttaccgg atgtggacag
    19021 acgggtaact ccgattcccc ggtccgtccg atcctcccgg ataacagccc ggattcctcc
    19081 acagtttctt ccggggaaaa gcttccggat cccgtaacga tcgttgtaga ggatgattcc
    19141 actcctcccg ctgagggcat ggtccgcagc cgtcttacga acgaatgggt ggatgccgat
    19201 gtggctgcta cacgccccat tgccgtaatg attcctaatg aggcatctgc aattccacaa
    19261 tacagtctgt ccgatgcttc tattatttat gaggcgaatg ttgaaaaccg tatgacccgg
    19321 atgatggcaa tctacgaaga ctggcagaat ctggacaaga tcggcaatat acgcagtctt
    19381 cgtgattatt atgcttactg ggcattcgaa tgggatgctt ttatcgtaca tttcggcggt
    19441 ccgtttttca tcaatgagct gctggcgcag ccggatactc aggatgtgga cggtacgtcc
    19501 ggcagtgatg aggctgcctt tttccgtact accgaccgta acaagcccca caatgcttat
    19561 gcttccggtg aagggctgca aaaggtgatc gagaaaaagg ggtacagtct gggataccgc
    19621 ggactcagcg atgagaatca cttccggttt gccaccaagg cggaaccgaa cacccttggg
    19681 cagtatggtg ccaaagcaaa ggatgccacc tatattgata tgtccggatg ttatccgctg
    19741 acaaggtgtt attttgaatt taacgaggat gacggcctct attaccgttc ccagcatctg
    19801 tccggaagca cagacggccc tcatatagat gccgccaccg gagagcagct gactttcaaa
    19861 aatattctgg tacagaatac gtttcacgag gaactgggcg aggggtatct ggcttttcaa
    19921 tgtcatgaca ccacccggga cggctggttc ttcaccaatg gcagaggaat acatgtgaat
    19981 tgggagaaga cttccgatta cagtgccacc agatattacg atgacaacgg ggacgagatc
    20041 atcctcaata ccggcaagac catgatctgt attatcgaag atggagattc ctttacgtt
//
LOCUS       DAWEJW010000052        19878 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1066_length_19878_cov_46.533320, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000052 DAWEJW010000000
VERSION     DAWEJW010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 19878)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19878)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19878
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1066_length_19878_cov_46.533320"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         6643 a         3450 c         4791 g         4994 t
ORIGIN      
        1 ggaaagtata ctccgtctcc agtaagacga gttgagattc ccaaaccaga tggtggtgtg
       61 cgaaagcttg gcataccaac agtggtagac cgtacacttc aacaggcaat aacccaacag
      121 ttagtgccaa tctatgaacc gctgtttgca gatggtagct atggctatcg tccgaacaga
      181 agtgcaaaag atgcaatact taaggttaag gagtatgcag aacaaggcta tacatttgct
      241 gtagtccttg acttgtcaaa gtacttcgat actcttaatc acgaaattct catcaatctt
      301 ctccgaaaga atgtaaaaga tgaacgtgta gtacagttga taaagcgcta tctgaaaagc
      361 ggtgtaatgg aaaacggagt ggtcattgac acagaggaag gctcaccaca aggtggaaat
      421 ctatcaccat tgctggcaaa tgtctacctc aatgagttcg accaggaatt cctgaaaaga
      481 ggtgttccat gcataagata tgcagatgac atcgtgcttc ttgcaaagag caagcgagca
      541 tcagagagac tcttggaaag cagtacaaaa tatcttgagg agaggctgaa acttacagtc
      601 aaccgagaaa agagccgtac tgtcagcgtg tttgcaatcc gaaattttaa attccttggc
      661 tttgcattgg gaaggaacgg aaaaggcatt tatgtccgag ttcatccgaa gtcatggaag
      721 aagtttaagt ctagactgaa ggagttatct tcccgtaagc gatgtcagtc aatcaagcca
      781 agccttgaga aaatcaaggt gtatgcgagg ggatggctta actactacgg aattgcaagt
      841 atgaagaaca acatagatga catcaacgga tggctctatc acagaatacg tatgtgtata
      901 tggaaacagt ggaagaaacc tagaacgaaa tataaaaatc tagtcaagct gggaattcca
      961 gaacactatg cggcaacaat agcaaacagt cgcagaaagt attggtatat cagtaataac
     1021 aaagctgtga tttgggcgct gaataaagaa agactgataa acagtggctt ttatgattta
     1081 gccacagcct atcagtctgt gcacgtcaac tattgaaagc gccgtatacc gaacggtacg
     1141 tacggtgctg tgagaggacg gcggttagtc accgcctcct actcgattta tacctatatg
     1201 tagaacagtt cgtgcatgtt gggctaccat tcgtgcatag agggattgaa aaaattaaat
     1261 actctgttat attaagcaca taccatggta aacaaataaa gttgtctgta attgtcagac
     1321 aattaaggag gggggggggt gaaaacgatg gacaataagc aagtaaacaa ggcaccccag
     1381 aaaagtgccc gtctcgtact tcaggcagtg actgctattg ctcgaagtaa tgcaaattca
     1441 ttgtgccgtg gtttgttgta tgaaccaaaa gtaccaacgc agttaaaaaa ataagattgg
     1501 gaggaattga ctatgatgtt aaaaagttta aaacagaaga ttgcaaaagt aggtattggt
     1561 ttgttttgtg caggatgtgt atttctttct tcagcagcat attctcaggc ggcatcttac
     1621 accgcttatg cacttaaact gaataagact aataacttta cctctatgca tgcaaaaaca
     1681 acatccgatg attacatcac aaatgaggta acagcaatta ccaatacctc ttacgcaaat
     1741 ttctgggctg tcaataacag caagagtaaa atatctaata agtataagca gggtgttgga
     1801 acaggatctt gtaatttaat ctttaccaag agcggttata attctactaa cacacaggtc
     1861 ggtatgggaa tggaaaatta tcaatctcaa ttatcaactg catttgtaag tggaaatgta
     1921 gattttagat aacagaaggg atgggcataa acctcttcgt gggtttatgc ccttttggta
     1981 aagattatga aaaaaatggt tcttatagaa atcaaacatt gtattgatcg tgcagaatat
     2041 aaagttgttt ttattgctct aatgatttta aatattgctt cgtatatttt atgtgtaaaa
     2101 aatgatatag gaaaaagtta tcaatttatt cgttcagcaa atgaaaattt cgtattacag
     2161 ggcactgaag cggcatatat tccatatata atgtatctgt tattgcctgt ttatgcaaca
     2221 attattgcat cactttcctt aataagagaa gaaaaaagca atagttcaat cctacttatt
     2281 caaagaatcg ggaaaaagaa atatttagca ggaaaattat ttggcattct gattgtaacc
     2341 tttttgacaa taacaatacc aatgctcata aatttattat tatgccactt gacatatcca
     2401 atacatggat atgacagtgc ttggggagaa cctgattacg ctataggttt ggtatcatat
     2461 aacgctgaca attttcttga tatggtgaga ttgcaacatc caactttata taatctaata
     2521 tacattttta attacggaat ttttggctcg ggattagcta tcttagctta tgctctttca
     2581 tattataaca aaatgaaagt tctatatttc gcaaagatac ctggtgcttt gtttatattc
     2641 tatattgttt ttaatttgat tgtttctgtc tttgggctaa atgaattctc tatacaaggt
     2701 tatattatgc ctaatgttca tggaaatata ataggagaaa ttgttctgat attgcttatt
     2761 tttggattaa gtattggcat tatacagaga aaaataaaaa attatgaagt tatataagaa
     2821 aaaaattttg aatgttaatg ttggaaaatg cttactagta tcacttataa catttgtaat
     2881 tagatatttt tatgctaatg tgtacttggg gtttcataat agtctggtta caataacaga
     2941 aggagtacgt tcacaaatat attttggggg aatactaata gatcattcgt ttgaccttat
     3001 atctttatca ttttttgcga ttttgtttat ccttgagatt tgttatctta caatggactt
     3061 atttaatgat gcaaatgata acatggtaat tctacttcct cgttatggga aaaaacacat
     3121 aatttataag gatatattaa taaaaatgat attacgtgac tgtattgtag ttgtttccga
     3181 ttatttgttt tttctttttg tagagcatac ctctaatggc ttagaagatt tcaaagttat
     3241 ttttatgcta ttgatagaga tttttcattt gcaaatgatt agttatttgt tttcattttg
     3301 gaaagattca attataggat acatcgttgc atttattgta ttttttattc cagtaatggt
     3361 gtgtggattt tatttttcaa tgggaaatgt tatatggaaa atttggcatt attccttgct
     3421 acatattcct ttatttaatt ggcataacaa gcttattata tattatgtcg aagaaaatta
     3481 cgaatggact acaagcaata tagatttatt gaattttaag tcaagctgtc tcatttcagt
     3541 tatatcatcc gctgttttgg tgtggatagg taaatttctt tgcgaaaaaa aacaaatatt
     3601 gtgaggtgaa tgatatccat gaatttatat gtaaaatcta tttcaaagtc aattaaaggt
     3661 gaaaaagtgc ttgatgatat cagttataca tttgaatctg gtaaaatata tggaatatat
     3721 ggtaaaaatg gttcaggtaa aacaatgcta ctcagagcca ttgcaggttt tataaataca
     3781 gacaaaggat acataaaata taatcacaag gcattacata aagatatgga tgttttacca
     3841 gatttaggag ctgttattga aaatatttct ttttggagta tgtattcagg atttgaaaat
     3901 ttgaaaatgc tttccaatat aaaaggaatt gtatcagatg aagaaatata tgaaataatg
     3961 atatactttg gtcttgatcc caaatcaaag aaaaaagtaa ataaatattc attaggaatg
     4021 cgtcaaaaac tagctctttg tcaagcactg atggaacatc caaaaatttt gcttttggat
     4081 gagccaacta acgcattgga tcagcatagc attgatttat ttcgtgaacg tatattggaa
     4141 gaaaaaaaga atgataccat tactatactg acaagccata ataaagaaga tattgaaata
     4201 ttagcggatg agaagatatg catggaattt ggtaaaatta aggagttgat gtaatgaaga
     4261 aatttgtatt aactatctgt gttgtaatca tttgtatggt gacagcctta ttctatggaa
     4321 agactgggat aaaaacaact acaggggttc atgatcctga tattgcttta tcagaaaata
     4381 cagctatgga aattttctat acaggagggc agaaaacttc ttactttaac aatgaaagta
     4441 attactcatt ggatgatatg gaaaaagatt cttctttaat agctcgtata actgtaacaa
     4501 attctcgtga aatgagttta cgatcaacaa aaacaatggt aaaaatagag gaaatctata
     4561 aggataattc tgctaatctt tcagtaggtg actgtattta cattatagaa cctgtatcat
     4621 ttgttagagg tgaagaattt tacactaacg gatggcaata tctgaagacc gaagatactt
     4681 atttaatatt tttaaaaaat ctaggatgta ttgatgggta taaatacacc aaagaggaag
     4741 aaaattcatt tatgcctgta tcggaaatat attcaaagag aaatctaact catgatgaaa
     4801 gttgtgtggt ttttgattcg gcggaaactc tctattcaag tatatctatg tttgcatttt
     4861 tgacctcaga aaaaactata gtagataatt atgagaaaat atggaaaaac attacaaatt
     4921 caggtaagta tgaatgattt aattatttat gaatgaatct ctcctattct atttcctgtg
     4981 agaagcgtat caatgttaga aattcatgga gatactgaat taagcaatat ggaatcattc
     5041 aatcttaaag aaactaaaaa tggagataat agaaattgaa taaaaagaaa ttggcaaaga
     5101 gtgttgtagg cattgcttta attgtaagtg tagctatact tatcgttgta tgtgttggaa
     5161 aaataaagca cattcagaat aaaaaaaata ttattggtac atggaagaat gcaaatgcag
     5221 aggaagtgtt tacctttcaa gaaaatggag gacttgttgt aaacaaagat atgcccgaat
     5281 tgggtattac ctgtggcaat gcattatata gcatatcttc ctcaaatatg atttttgtgg
     5341 aacagagaga taatagcgtg aattttgaga tagaagtaga tcaaaacgaa ttaacaatct
     5401 tctttatggg tcataaatat tgggaattaa caaaatagca attgcatata aagcgatact
     5461 catgatatcc ataaccataa aatatagagc cgatgaatca ctcgttattt cgattgtgag
     5521 ttatcggctc tatttcatta tttatggaag ggctaaagaa atatcgtatt ttatagaaat
     5581 acttttgaca gatttcttct catttttatg ttcttttatc aagtgctata ccattccata
     5641 ttcatcgcat tcacccaatc ttgcatttct gaatcaagat acaaacgggc atattctagt
     5701 ggattgtcaa tcgccagtgc atccaacatt cgttgagagt taggagtagt aatcaatcca
     5761 tcttctgcct tgccacagtc aatccgaagt atgtagccat tgtaataatt gatgtctatg
     5821 atgttgtgat atttattata ttctgcatga acgatattca tattgcaaag tcctttctct
     5881 tatctgtttc agttgtagaa gcatcttgac cagttccagc cgattatgtg atttatataa
     5941 tgttattagc tgttaccgga ttttgcttcc cttgtttttg cccttatgag gtgtcgtaac
     6001 acaagggaaa aggatataac ataagggaaa gtctaaggga aagctcactt gccaaaactg
     6061 tagtaaaatc aaggcattgc ggattttatc gtaacacttt ataataccag ttaggtgtta
     6121 aagaattgta ggtatcaaaa ttttgatttg cagaaataag cacagtatca atattccact
     6181 cattaatttt aggaataatg cgtttcaacg agacgttttc gatatccgag ggtaacatag
     6241 tcgaagaacg gatactgtta aagattttag tgaatcccat ataaatttcc ccttttcata
     6301 ttagtattta tatcagctcc aacacaacta tgggtcgaag tagataacat tatttgtgtg
     6361 ctccgatctc gatcatatcc acggagcatt tcctgtcgta gtccccattg atgatatgtg
     6421 catactcatg caggtaagat ttccggttct gttcgaagga cagagcatcg ttcaggacga
     6481 tggtaaagct catatcggga tttgccacca cataggcctt gatgctgtat ggcagcgttg
     6541 ccagtaccga atggatatcc atatcagcca cctcctcaga atatgtatga gccttggtca
     6601 tccgttctgg ttgctcattc ggtcgatcat ctcttttaca aactggatgt cctccggctt
     6661 caccttacgg gaagcatcga agaggacctt gtattccgga ttttcaaaga gaaactgcgc
     6721 catatctctg gcatcttcat tcaaataata tgatgcagaa tccgcagatt gactttctaa
     6781 taaatcggat ttttctatat gaagccagtt gcagatagat tgaattttat ccattcgagg
     6841 cattttctta ccgttacacc aatcagatac ggttgcagaa gaaacgccta tgtatttaca
     6901 aagatctgcc tggtttttgc cgtttatttc caaaaaatga agaaggtttt tggaaaatac
     6961 ttttttgtat tcttcatctg acatttaatg cacctccatt tgctatgatt ataagctaaa
     7021 agcgatgaaa aatcaataca aaacacaaaa tatttaactt ttggtattga cagctagcta
     7081 aaagcgagta taatgtaaaa cagaaaggcg gtgatattta cttgaaagtc tcacttaaag
     7141 cattacgagt gaatgctaat ctgaatcaaa aagaagtagc ggcaatgatg aatatatctc
     7201 cgaatacgct tatgaattgg gagagcaact atacttctcc agatgtatta caattatcaa
     7261 agctgtgcac aatttataag tgtacgatag atgatatttt tttgcctgat aaactagcta
     7321 aaagctagta ttttagtaaa ggaggcagca ggaatgaagg gtgataagaa gacaaagaga
     7381 aacatgttgg ccattctaat cactatcatt acggtaagtt tgttaaggca ccttggctac
     7441 tttgggacaa cggaatatga attcagctgt ttgtatatgc ttatatactt atgcatcaat
     7501 gaagtcttaa gagagaattg atcagcgctt taagttcttc cgtatacatg ctcagcaagt
     7561 atgctattat ccagccgaaa aggttcagaa gtttagcaat ggccggcttg gttttgaatc
     7621 ctagttgttt aaggatagaa ctaggtaatg aaatcatgat tttcaaagca ctaataggat
     7681 ttaagcagct gataatgtct ttcctgagaa aattcctttg catgaggagg tcattgtata
     7741 gattgacaga ggaaaggtag ttactataat ctgacgcacc atatccaaga gaatctgagt
     7801 aataagactt gaattcacat atgtcgggat attttgcaag aacggcattt aacttctctc
     7861 gataagaatt accggttagg gaatgagaat atgaagcggg agatgtggac tttaaaaaat
     7921 cacacagtga gtctatgcag gaggtgattt taacaaattt agcagcattg cgcaaaattg
     7981 accagaaata tatcacaatt aaaagaatta caaggaattt caacattata tgtaacctct
     8041 catatgaatt tacttggctc tggcgggagc ctgtaaggaa agtataagag aaaacaaaag
     8101 tgaatgcaac tatcatctca tcactaataa aggaaggagg cggcaggtat ggataataaa
     8161 agagactgga gtgcaataat catgtctatt gtttcactgg taacaagcat aattgtattt
     8221 gtgctgacag tcattatacg actgatgaaa taatcgaaaa aacaagcgtt aataaagaaa
     8281 ttataacgga tatcacggac attgtttttg caaacaaaga gtcagtgcga gcctgtttag
     8341 ctaatcgatc agcgctggta gcgtggagat cattcagata tttatatcct ttaagagtaa
     8401 gccctttggg aaattcaaca tgaggatcaa agttgccatc acgccattta tcaatgtaaa
     8461 caataatatc gttttcggca aactgataag ctaattcgtc gatttctttc tgggtatgac
     8521 caagcacaga tatttctttg tatggcgtac cgttgataat ctgttccaga acagaaatgg
     8581 aaaagtcaca gtgtttttcg taggaacttt gatttttcat aaaaacctcc atacttttga
     8641 gactgataac tgaacactat caattataga aaaagaggat agaggatgca agagatttta
     8701 tagggaagga ggcagcaggc gtggaaatag taatccacaa agaagagagc aggaattttt
     8761 tacaagtagg aaaagaaatg atagaggtat cagattatag tttaaaaagc tccgcagacg
     8821 gttccacaga gctttctgta actataaagg gaaaggtcaa tgtatttgag acatcagcca
     8881 acctaataat gtagaggaaa ttgcgcttgt aaggagtctg tttgtgtagg aactgttttg
     8941 catccataaa gacaagttta tcacaagaat gggagttgca aatgaacttt tcaaaaagga
     9001 ggaataacag gtgacatgga ttcttgcaat aatagcagcg atttgtgcaa caggttgggc
     9061 gtttagtcgg ctggcattca aaacactggt gcgatgtttc aaggaaaagg gatatccaat
     9121 ggttaaagaa aaagatatcc agaggaacat ggtcagcata ataaaagaaa ttaactgggt
     9181 accgattgct tctggaatct ttggagggtt actgggagta attctgtcaa aagtaatttt
     9241 ctaaatttaa gaaggaggaa taaccaatga acaaagtaga tgaactgatc gatgcgctgg
     9301 cggagcatat caaaaagcgt atcgatgaag gtaatgacat ggagaatgag atcaccgaaa
     9361 agacgaaggc tctcgcagag ctggtgtctg cgagaggcaa agaaattcct attgactgga
     9421 aaaaactaat gaaagccaaa gaggagtttg aaaaaataac aggaattcaa atttaataga
     9481 aaatcagttt taggagtgcg gatagcaccg gaaaattgta tggaaggagg ctgcaggagt
     9541 gtttccaaca ataagagaag cagttgacca tagtgtctac tatacagaaa tagagacaca
     9601 caaaaaacgg tatgtcaaaa gactgttcga agaggaagag atagaaagag cagaagctat
     9661 agtaaccgca ctgaacggat tacaaataga ttctgctcag gagttgctga aaaagataaa
     9721 catgtatctt cttcaggact caattaccgt atgaatagat ttcagagatc tcgttgtaag
     9781 cctttttgaa tttctcaaag aattctgaag catccatctg agaaacatca taatgggcag
     9841 tgacataagc gagagcaatg cgatcaatac ggtcatcaat ttgttttact tccatggttt
     9901 ttctcctttc gttgtactag gctctggcgg gagcctgtaa gtacagtata ggaaaaggag
     9961 aaatcggatt caatatggca ggcatttcga tttatcgaaa gaatcatttc ggagaatcga
    10021 agtgcagtag gaaggagatg acaggaatgg agaaaatcga cagattatat gctctcttag
    10081 agcgtaacga cattgatgag aacaccaagg cagcgctgcg gtgggcaatc tttgagttgg
    10141 agaatgcaac ttagacaacc atggcaccat aagctgtaga aaagcagtca ggaggtacat
    10201 atgcggattg taaatttaat ccacatcggg gaccaggtat tgtcactgga tgacatggat
    10261 cccatgaaaa aggcagaaat tgccttacgg ttgaatgaac agagtctgaa gactttggga
    10321 tatgcagcca agaagaaaga ggaatcagcg taaccacagg tatccgtgcc ctgtacgtgg
    10381 tgtattccca acaccacact ccccttttac acaattagcg tgtgtgtcca ggcatcccca
    10441 cctgggcacc acgtagaggg catggacaag cataacagat cacgttctgt gcgtggtgca
    10501 tcttgctgca tcaccatatg acggcatatc accaactgct atgatggtat gccgatctcc
    10561 ttccggtggt acccgggtag atcagcaccg gggccacgca gagagcgtga tcagaaaggg
    10621 ataaacatgg aattgattaa gcattatgcc aaggaagttg tgaaaaacaa atacggtcac
    10681 aaatactggg aagctagcaa ttcgcaacta gccgagtatg tctatgatga agtgaaacag
    10741 caagtgccgg aggctaaata ttataacttt gaaggattgc agatcattac gacgaatgac
    10801 aagcaggagc agtcattgct gagcacactg gaagtgatgg aggatctttg caatgaaagg
    10861 ataattcaga tacatagact gagagatcag atatatggag gggatacaga tgtataaaga
    10921 tattgtaata tcacttcttg gggcattgct tctggagccg gtacttaaga caacagagac
    10981 aggagagcag atcgccatgg tcatgggctt ggcggctatg ctttttattt tttgcctttt
    11041 ttgcgaggat caggtggaaa aatggcgaaa ataccgccaa agagtacagg atctggaaca
    11101 gagaatagag cagatgagag gaggcgggat gcgtgaaaga cgagagagtg aaggaaatca
    11161 tggagaggtt ggaacagacc ccgacagagc cattaatgat gctggttgac catgaggcac
    11221 aggaggtttt cccgtatgtc ctccggaagt atcagaatgc acatctggtc atgatgaagg
    11281 gcatccggta tatcacaatc accgatgatg ccatccgagt catactggac cgcctgcaac
    11341 gagagagggc ggattttaaa cggacagtgg agtactacga cagggagatt cagggcgttg
    11401 agtatctgct gacaggcaaa aaacggtact attggtcacc ggataattac atagtagagc
    11461 ctgcctatgc agagcaataa aaaagccggc atttggcgat gccggccagc tcacagagct
    11521 acacatataa acaagattat tgtaactctg taagccgaaa aagtcaagaa aaatggggct
    11581 ttcgaaagcc cctgcgcact tgataaagat attaaagtta ggatacagag acatggttaa
    11641 gagaaagaaa atgagactga agcatgggga tgtcctggac gtggaagagt accatgatgg
    11701 gaattatggg gggaagggta agaccagaca gaagaaggag aagccgacga aggaacaggt
    11761 aaggctgatc aaccgaagaa ataaggcaag gctgtgccgg tggagactga tacagtactt
    11821 tgaccaggga gacctgttta tcacatggac ctatgcgatg gagaatagac ctcctgatat
    11881 ggcggaagcg ctgaaggact ttcagaaggc aatgagtaag atccggaaga tctaccgggt
    11941 aagaggagca ccgctctact ggatccgcaa catcgaatgc ggcaccaaag gagcctggca
    12001 tatccatctt gcgatcaagc agacaccgga gggagatgca gcggctatcg tgaccaaggc
    12061 atggaccaag ggcggtacct atgtggcgga gatccggaac agcaaattta acggggatga
    12121 catggagcag ctggcagact acctgaccaa ggacgagcac acagcggaga tcaaggtgga
    12181 tggcacatcg ggaaagccca gaattgcgga gtcctcctat aataccagcc gtaacatgcc
    12241 gctcccggag ccacggacgg acaagctggt ccgctggaag ccggaagtca aaccacccaa
    12301 gggatattac atagcccgga tccatgaggg catcaatccg gtcacgggat ttttgcaccg
    12361 gagttacacg ttgatcaggt tgaaagcgac agagcggaag aaaccgccga acaaggtaag
    12421 gaggtgttga ttttggaaac tatagaaatg tatgtgagta ctactcttca tggatccgca
    12481 aagaggaccg ggaaggtaat gtatactctc cggatgaaaa tgggagaaaa gcagtactat
    12541 gagaaaccgg cggagatagg aaaggcggac gggacagcca accggctggt gctgtggagt
    12601 atctgccggg cactggagcg tatgcggggc aagagaacga tccttatcca cacggagaat
    12661 acttacgtcg catccgtgat caatcaggga tgggtggaga catgggagag aaacggctgg
    12721 aagaacagca gagggaatga aatcaaggat gcggatctct ggaaaacaat actggagaaa
    12781 tcccggaaaa tgcgtcatga catcgctgct gtggccggac agcatgagta ctctgaggca
    12841 tttgcctaca atatgtctaa aataaacgca gaatataaca tttttgcaaa agtggagttc
    12901 gaagaggtaa caccagtaca tgacaggtgt tagagaccat tccagtgaag tcgccgagat
    12961 ggtgagaata taacaatttg acagaaaaac agtcggttac aaggtgaaac cgactgaact
    13021 actcagaaaa aatcggtagt tgcaccggtg caaccgggga aaggagaaca gatggagaag
    13081 aaatttggaa tatttaatac cgtagaggag ttaaacaggg cagcagccgc ccagaaggca
    13141 gagggagacc tggaagcgct gatcgggatc gctgttgaga acggactgga gaaagaggat
    13201 gccgaggaat acatggacag cgacgaccct gaagattttc tctgtaatgc cacgatggcg
    13261 gccattgcca agctgaagct ggaagaacag gacctacatc ttgaaagcca gttaaaggac
    13321 tggaaggact ttatcgtgca gatgctgacg gagtatccga tagatcatgc cgatggggac
    13381 agggatgctc tgggaaatgc cgtatttaat cctgccaagt gtctgctgga cgtactggca
    13441 gcaggaatga agctggcatc tgaacatcgc ataaaagtag ataagagaat catagagaaa
    13501 gcaggattgc cggaaagagc aggggacata ggatccatcg gccgtgatga gttcaagaag
    13561 attgttctgg attattacat gggagaaaag aaatgattgt atacaaagca acgaacaagg
    13621 acatgatctg cacatccggg gagggattgt tccagtatcg tctcggagta ccagaaacgg
    13681 cagaaaaatc aaagtgtgct gataccggtc tccatgcctg tgagtatgtt ttggattgca
    13741 catggtatta cggactgggt aaatataaca ggtattttaa agcaaaggca gaaggggaca
    13801 tagcagaaga cggacacgat acccgcattg catgtacgag gctgacactg ctgaaagaac
    13861 tgaccaaccg ggatatcgca aaagaagcca tgctttacat gatacatcat cctcgacggg
    13921 ataactggaa gacatcccgc tatatggtac aggtaaatga gaacacagca gagatcagga
    13981 tcccggacgg gatcgccatt gcaaggggaa tccatcccaa agtaagcggc tgtgccggag
    14041 cgcatctggg gctgatccgg gaagaaaaag gaaagatcac ggcggccaag atatttgatg
    14101 tggacggagt ctatatcctg ccgggagtgt ggtacaccct ggaagacctg gcagaagcag
    14161 agaggaggca gcagtcatga agtggacaga gatactcagg gcaccgatga taccggcaga
    14221 taaaaagaga aataaacaga tcacgttcca gacaacggag aattatctga tactggatat
    14281 ctggaggggt ggaaacaaca tctgccgcca tgcaatcaac ctgaaaacat gggaatacgg
    14341 cacatatttt ccggataccg gcataaagca ggcaacaaat atcaacagct gtacagataa
    14401 ctatgaaaga gagtactggg attaccggct gaaagagaaa gaatggctga caccggagca
    14461 gatcagggaa ctggatatcc ttaccagaga aaagaaggat tgggtaaaag atgtattgca
    14521 gcgcatagag cggatggaga cagactataa cgcagaaaag cgggaacagg ccagaaacag
    14581 taaggaggag cgcatccgta gattaatgga aaaatgtcca aatccgggaa aagcagtata
    14641 tgactggatc acagaacaga tggtaggaga tctgcagtat gccttttatg gcaaacagaa
    14701 aaagacctgc cactgtacgg catgcggcgg aaattttccg gaagaggcag caggcatccc
    14761 tgtgaaacat aggaagcaga tcacctgccc tctgtgcgga catctcctga ccgtggataa
    14821 gagagcggat atatgcatcg ttgcaacgga ctggcttacc atgatccata acgtggatga
    14881 taaacaggga gtggagcggc actttaaggt aaaagtggag tgggacagat acggaacgag
    14941 aaccacggag ctggaggagc atatccggct gatgatgctg cggaacacag caaagaatat
    15001 catgaaagtc tattactatg ggagtgatta ctggcctgga tggagcacgg gaaataacag
    15061 cagccgaaga tggcacagtg cctatctgta tccggacaca gaaggtattc aggccggatt
    15121 gcatgggacg gcatatgagg tatggacaga tgtatttccg atgcttgccc agatgggaat
    15181 aaaagcgcac tataacggtc tcatggtgga aagtaatcgg gagtttaccg gtattgcgga
    15241 atatatggca aagggacgct tttaccgcct gctggatgaa ctgtcacagt gcatcaccta
    15301 ctggggtgga tattccggga gcacgattga tgtatctggg gaaagcgtag aagaaatcct
    15361 gcagatagag gataaacagc tgatcaaccg cctcagacag gcagacggcg gaatgtgtat
    15421 gctgcgctgg ctgcagtggt ccgacctcaa caaaaagaag ctgtcagagc agtacatatc
    15481 ctgggcagaa aaaaataaga tcgacccgga taattatctg cggtcggaag caaagcaata
    15541 cctgacaccg gaacaattaa tgaattacat caaccggcag aaaaaagaaa gctatccaag
    15601 cagaacgata gcgggagtct ggaatcagta cgaggattat ctcagtatag caaaagaact
    15661 gggaaaacac atggatgatg ctctggtgca ccgtccaaga gaattgaagc gccgacatga
    15721 tgaagtcaat gccgagatgg aattgcgcag ggaagagatc cagcggaaac ggaatgcaag
    15781 agaagcggca agacaggcac aggaaatgag ggacaagtat ccgggatatg aggatatcct
    15841 ctccgagatc agcgagaagt ttgagtatca gaatgacacc tattgcattg tggttcccag
    15901 ggactttatg gagattacgg cagagggcat ggcactgcat cactgtgtag gcaatacaga
    15961 gcggtacttt gaccggatcg tcagcagaga gacctatatc tgtttcctgc ggcagcagtc
    16021 gtccccggac aagccatttt ataccataga ggtagagccg ggcggaacga tccgccagca
    16081 ccgcggagcc tacgacgagg agccgggcat agaggagatc aagccgttcc tgcgggagtg
    16141 gcagaaggtg atacgtaagc gcatgagcaa gcaggatcat gaatatgcag cacagagtga
    16201 gatcctgcgg cagaagaaca tcgaagagct gcaggcaaag aataatactg tggtcctgaa
    16261 aggactggca gaagatctga tggaggtaat ctgatgggag aaatcataag ctatgaagaa
    16321 agatacagga agtataagca ggagttggac ggagcattta cccaggcagc agagaaattt
    16381 gtgctgatcg gctacctgct gagagaggca gcagaaacgg acatattaag atccagcgga
    16441 tataaaaaca tggaggaatt tgcctatgca gagtacggtg tggatgcatc ccaggcaaac
    16501 cggtttatca atattaacag gagattttcc gaaggcggta attcaaagca gctgaaacag
    16561 cagtaccaag gcattggaag ttccaaactg gccgtaatgc tgaccattcc ggatgaaatc
    16621 aacgaagttt tgcctaaaaa cttgaccaaa gaagaactta aggaaattca ggcagaagta
    16681 aaggctgaaa atcaggtatc tgacattgag gtggaaatcg agaaggcaga ggcagcagcc
    16741 gtaacggact ggtccatact tccgccaaag ggatcgcccc taaaaaggaa cctatggcag
    16801 ttggggaaag aacaggaaga tctcttccgg aagttgtggg aaatatgcaa taagggacag
    16861 tatccgcata gcgcatcaat tatggatgca ctgatcccgc agggggatgc ggtgtacacc
    16921 gtccggatcc cgggagagcg caggacacag atcattataa attctgacgg tgccacggta
    16981 atcaacctga agacgctgga gcggagcaag tacataccag aggacatctg ccatgaagta
    17041 gaagaactgc ttaatggagg cagcagtcct gaggagcagt acaagatgct atattgcgag
    17101 aacatgactc cggaagaacc ggaagttgca ccggtgcaac cggatgagac cccgaaagag
    17161 aagaaaccgg aaaaacgtaa ggaatcccgt gtaaccaagg caaacaccga aaagaaaaag
    17221 cccaaggaac cggaaaagaa gccggagcag atgaccatcc cgggagccgc accggatccg
    17281 gcacagaatg aactggaaac acaggtaaat gacacggttt gcgggaatcc cgacacggct
    17341 aataaggatt ttgctacaga tcatcagaat accgaagcta tggtcacgga agaacaggta
    17401 cctgggcaga ccgacataga aaaagacttt ccgcaatact gtccggacac cgtcgaccaa
    17461 aggtcagctt atcagcagtc cttacgtggc gctgtggaga acctggtacg atatgtcgag
    17521 atggatctga tcgccgccgc cagacagcag ctgtccgata tctccggcta tttggacaaa
    17581 ctggaagaaa tcagcaaagg aggcggaccg gatggcgaag ctgtcgaaac aggcgagagc
    17641 gcgggagttt aatacatcct cccgacggat catcaaagag cgagaccgca accagtgtat
    17701 attctgccag atgcaatacc acatggaaga tgtcacctgg tcaggtccgg aatcactgag
    17761 catcatgcac tacatcccga gatcacacgg tggtctcgga atcccacaga atggtgcaca
    17821 gggatgcgtc agccaccatg agatgctgga taacggcaac aagggccggc gggaagaaat
    17881 gctgcagatg tttaggcagt acctgcagga tcactaccag gactggagcg aggaggcgct
    17941 gacctacagc aaatggaaat aatgtatata caaatttgta tatacaaaaa ttggaggata
    18001 tatgaaagca aagacagaag ttatctcact ccggctgaca ccggaggaaa agcgcagatt
    18061 ggaatatagt gcagaaaaaa tgggaaagag ccagtcatat atcctgacca cagcattaaa
    18121 tgcatattac aaatctgtcc ggaagaatct ggacggtgta atagaagaat agaccttttg
    18181 gagtgtactc acaatcactg taaacatagc cacggggcgg ccgctgagac caagaggcag
    18241 cagctgtcca gaaaggagac aataatgcag gagtacaaag agtggactgg tgatatcctg
    18301 ccggatcccg cgccgcgcat ccgcaatata catataggtg acataatcaa aacagtacgt
    18361 aaggtaatcg aggagccact ggaaatccgc ggacgtggtc ggcatcaggt cataagcgag
    18421 acaagagaat acgaggtaac cgcgatttac ccacatatga ttcagaccag cgactgcaaa
    18481 acaggcttta cgaggtgttt ttcctacggc gaactcacaa cgatgggact ggaatggcag
    18541 ggagaacaga aatgaagacg gtcgaaaaga aaattctgcc caagtacttc cgggcagtcc
    18601 gggaggaaaa gaaaaacttt gaactgcgga aagatgaaga caatgtacag ccgggagatg
    18661 tcctgatact tatggagtgg gaagatggag aatatactgg acggacagag gtacgccgga
    18721 tccggtatgt gctccgggat gtaccagagt atggattgat gccaggatac tgtatcatcg
    18781 gatggtaaag gaggatgcta tgaaaaataa aaatgtgtgg tttgcttatg cagcagcctg
    18841 gatatctacg gcaacagcgg tgatatttgc tatcaaatat accggatcag cgtggtgttt
    18901 agtggcactg gtgctgccgg caatgcaaaa gataagtatc agcaatgatg aagagaatgg
    18961 taaataactt agaatttaca ggaggtagga catgagctgt atgcgatgta tctgcgagca
    19021 ttgtgcaaat aatccaaact gctttgacca ttgtcagaga gagatggatg aaccgtgcta
    19081 taactgcgat gattgcattc actgggatgg caagacagga cgggagatgt ggagggacga
    19141 gtgccctaag tataagataa cggagtactg ggcagcgcat ctctggcgca aaatgaaaat
    19201 catttaggat ttagtggagg tagagaatat gagtaagaca gagatctgtc agatgtgtga
    19261 taactattct gtgcgcaaca agtgtgatca gaagaaagat tgcaaaatca tgaaaattat
    19321 ggatgaaaat gcagcattaa aaaagcaggt gaaggaatta aagaaggaac ttgcggaagc
    19381 aaagttaaat atgtcataca tgatagatcc caatgccatc ggcgatagaa atgatatggg
    19441 atggtagttt tagaatttaa tggaggtaga aaaaatgtat aaagcaacaa atattgatac
    19501 ggacaaggct ctcaaagcaa tcaatgattc aagagcaata caggaaagag cgtcacagct
    19561 tagatcggaa aaagaaagat cttacatgga gggactgaac aaaggacttg atattgctga
    19621 aagtcttttt gaatgttcaa attatgagaa atcggcgcag gaggcaactt atacagatgg
    19681 tgtctgcgag gtactctatg aacttggaaa agaacttgat ataccaactc aggatataag
    19741 agataatatt gcatcggtag atgaagcctg cgctctgttt gcagacagga ttcgggaagc
    19801 aatagcaaga gataaggatc agtaaactga aatttatata attatctctc taactttccc
    19861 tattttatag gctttccc
//
LOCUS       DAWEJW010000053        19858 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1067_length_19858_cov_63.914104, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000053 DAWEJW010000000
VERSION     DAWEJW010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 19858)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19858)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19858
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1067_length_19858_cov_63.914104"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2811..2910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4828 a         5099 c         4080 g         5751 t
ORIGIN      
        1 ttgtcaaaga tcaaaacctc ttgctccgca agagctgtgt tgctttgctg ttgttttcag
       61 cgacagcttt gatagtttat cataaccgtt tctgtttgtc aacaactttt ttcaagtttt
      121 ttgacttctt acaacagtca acggagaagg agggatttga accctcgcgc cgctattaac
      181 gacctgcacc ctttccaggg gtgtcccttc aacctcttgg gtacttctcc agatagttga
      241 ttctaattaa tctatataat tatcggcggc acgaatgcct agcggagaga gtgggattcg
      301 aacccacgcg ccctttcgga caaacggttt tcaagaccgc ctcgttatga ccacttcgat
      361 atctctccga atgtgtgtct taagtctttg tcatctgtgt tgctcacctg acgacaaaag
      421 ctattctaac aaaaaggtct gctattgtca acacattttt acacaatttt tattttattt
      481 ttttaagatg agaaaaagcc cattttatca cgtttttggg cttttcctct ttttgctgaa
      541 tacagttttt tctatgaatt gtgtctgtta tcatgtaatt gttacaaata ttcctactat
      601 ttgcttttta ccactgtctg cttttccctg caagatacaa ctcccagtcc gtcatatgat
      661 tttccatatt agtgatcaga accactttgc agtcattctc agagtaggtc acttctctct
      721 gccacttgta aaaatccggc ccgtctccca gatttctcat atagcccatc aggcgtttca
      781 ggcagtaagc tgtctccttc acatccgtca ggcaatcatt ggcataaaag tctttccatt
      841 ttccgtgttc ccggtccctc atggcgctgt ccgccgcttc aaatgcttct gcagcattcc
      901 ccaacaggaa gaaggccttc ttatattcct tccgttcata tgttgcaaat gcttctgtaa
      961 ataagatagc tccctgtaga caattcctgt ggattttcac ctgcagcaat acggaatctt
     1021 tccataattc attttctcca acggtaaggc cgcgctcata caaaccctca agctgttttc
     1081 ccgtctgcag acattttcct gtaaaccatt ccatctgtgc agcaaaggta tccttatgaa
     1141 tacaccagaa cagttcttcc accggttctg cagttccgtt cttcatccag ctatagataa
     1201 aatccctcac aacataatta tagaattgtt ctccggcatg ctcatcctct ttctctccaa
     1261 agggtaacat agccctcgga taatcgcgaa tgcagtcttc cataatggaa agctgcgcag
     1321 tatcattctc cgccccatag tattcggtga cataacgctt tctatgctct tctgccgaca
     1381 gagcatcctg tttccacaga ttggcaataa aagaaagagg atatacatgc ggcttgatat
     1441 tggatgcatt gacaagccac aggtccgtaa ttccatgcct catagcatcc gtaagctctt
     1501 tttccacgaa ctccatggaa ttaggcagca tggtaatatg gttggccgcc tgtaaatcat
     1561 agaaggatac atgataatag gtgccatgcc gtcccttatc tccttcctcc ggcaacgctg
     1621 acactctggg gttatgattg ccttgacgtc tggataccat cttgccgtaa ccgttgtccg
     1681 cccagatcag gatcacatcc tccgggatct gcaggcagcc ctcacgatac aattccagaa
     1741 tctctccgta cagattcgta cagaacactg catccggaat ctgctctctg accatggcat
     1801 attgcttctt cataatattg ctgattagct ctccccgttt ttcaggtgtg tcaaaagcag
     1861 gatcattttc ccagaaagga acatctcctt gtccccgaaa cccgatattc cagatgactt
     1921 cctcatcctt ttgccgtccg atggcgtctt tccataatcc ttcaaacaaa tctttatgtt
     1981 tcaaatacga aggttccaga tccgggtaag cccgcaggaa catttccgct cccagcggct
     2041 ccgcatggtg atgtgtgatc atcagcccca tatccgacgc aataggcgca tagattctgg
     2101 aatttttatc tgttccggga atcaccagat taccgccgca gcgaagcagt gcttcaaaca
     2161 ccatttccca tggatagtct tttgatacgc cggctgtcca atggctgatc agtacttcat
     2221 cattaatgaa ccagccgcgg taccggatcc tgtccgcctc tgaatggtat gtaccgcaag
     2281 gaatctctac ataagatttc acttccatct tctgatcatt ccagaaccag aaaggcagca
     2341 ctccaaggta ggtttcacta aggtaattta atgcatagat aaaggaacgg gcatccgctc
     2401 cgtagatcgt aatctcctgt tctgtcactt ccagctcata ttgttccggt ttcagatctt
     2461 ttttgcacaa aatgatcctg tttctattct tgactatgtc agtcaaagtc ttttccatgt
     2521 ctctccggag tcgctcacag gcaagttcta cggctttttc tgttacggca tccgaaagta
     2581 aggtagtgtt tctacttaac tgaaatgggt tcatgctctt ctccctctgt ctgtcataat
     2641 gatagcaaca ttataaaaaa atggggttct tgtatcaaga tacaaaaaag cccgtttcac
     2701 tgacaaaatt tgtctgtgaa acggacttct gtattttttc ttaattatct gccggattac
     2761 agtttcagat catccagcag catctctgca tagattccac cgatctcttt nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtaggggtat caccatccat gatcatatta
     2941 cgtaactgat actggaagtt aacctggtca ggattggtaa tatagatctt aacggtagaa
     3001 tagtcattcc atacggttac tgcgaacatc aggccaatgg aagccagagc tgctttggac
     3061 agaggaagca cgatcttgaa gaagattccc ataggggaac atccgtcgat ctcggccgcc
     3121 tcgaacaggc tggcaggtat tccttcgaag aagttacgca tcagtaccag gttgtacacg
     3181 ttcataccgt gctttaccac caggatccac atactgttaa ccaggtgtaa ctgcttcatt
     3241 accagatact cggggatcat accaccggag aagatcatgg taaacagaag gaaatatgcg
     3301 aagaagttac gtccgggcat atcaaactgg atcagtacat agccacccag ggtggacagg
     3361 agcagaccaa ggaacgtacc gagcacggtt gtgatcacgg agttgatgaa aggtctgtaa
     3421 agtctctggg tatgaaggat ggtcttatag ccatctaagg taaaatcctc cggccacagt
     3481 ctgaactggt atacacccac agcattgaag gagtctacta ctaccttcca gataggtacc
     3541 agaaccagga ggccgatgag aataaatacg atgtagttta caatatcgaa tacatgaacc
     3601 tttttcttag gtttacgata agctgtcttt gccacgttta acacctccta tacaatgcca
     3661 cggccacggg tcttcttact agcccagtta catatcagta ccagtacaca gccaaccagg
     3721 gacttgaaga gaccaactgc tgtagagtaa ccatagttcg gcagagcgat tgcgaatgtc
     3781 tgacgataaa tataagtaga aataacatca gatacgctgt ataccgcatt gttgtacaat
     3841 acgaatacgg actcgaacat attcataacc ttagcaaggt tcaggatcaa tacagtgata
     3901 atagtattgg acagtgcagg cagcgttacg aaacgcatct tctgcatacg ggttgcaccg
     3961 tcgatatcag ctgcttcata cagctccggg ttgatacctg ccagagtagc caggaagata
     4021 atggttcccc aaccggtctc tttccagata ttgataatgt agaagatcgg tctccaccat
     4081 gcagtgcttg ccaggaaata aattcccttg gcatccggtg ccaggatgcc ccagtcacgc
     4141 agcaatccgt ttaccagacc ggtagaggaa ggagacagga acaggctgaa gatagatgca
     4201 actactgccc atgacataaa gtggggcaga tatataatag tctgcaaagt tttctttgca
     4261 aagagatttc ccatttcatt taagaagatt gatacgataa ccgcagtgaa ggtcgtgatc
     4321 aataacttca tgatactcag ttccaatgtg ttcctgaatg cactccagaa cgcatcggtc
     4381 ttaaacatac tgtaaaagtg cttgagtccc acaaaagtag gagcacctac gggcttgtaa
     4441 tcgtagaatg cataacgaat accaagcatg ggccagtaat acatgatcaa aataaatata
     4501 aatacaggca ggaacattac ataatagcct ctgttattcc agatccggag acccaaaggc
     4561 ttgtgacgta cttctacgcc ggtggatgtg gtgattttct ttttcttgct cataccatcc
     4621 ctcttccttt caacttcatc tcacacattc ttttctctct aaaatatcct gtcccatggg
     4681 ctttgtatcc cgatgggaca ggataattat atcatatttt gcagcttccg ctactctatt
     4741 acggtctcaa ttactgattc agttcagcta agcactgatc aatgatactg ccaacagtgt
     4801 caacgtaggt ctgcatagct gcatctacat cgccgccatc aactactaca gaagtgatag
     4861 ctgccagctt agcatcatag atggtaccgg actcgttggt gtaggtctca gaggtaggag
     4921 actgaggagc atccttgcag ttctcggtga agaacttgtt gccttctgca gccaggctgg
     4981 agatatcgtt aaagccgttg tccagagagc agataaccag agcgggatcc atagcgttct
     5041 tcttccatac ggagttagga tcgttaggag actgctttaa gtggaactga ccatcttcgt
     5101 actcgtaaga cttctctttt tcagtacctg cattgatggt gaagctttca gccttggtag
     5161 accagtgaac atcctctgca ccgtaggtcc acagtgtctg caccttgtca ccatccatca
     5221 tggtctcgat gaatgcatcg aagatagcct gctcacggga attgtctccg tcaccgtcat
     5281 caatgataac ccatacagga gcttctctgt tcaggtaagc gccaacctca gcgataggag
     5341 ccagctcaac cagcttctca tctacgttgt tcttgatcag gttgtcggtc agagtctgat
     5401 accaggaacc tgcccagtaa gtgaatacac ctgaagaacc ggtctgatcg ttagagaacc
     5461 atttctcacg agcgatcttg gtggaagcgg tcagggtctc agggtcgata gcgccatcct
     5521 tgtaagcctg ctgtaatctt aacagagctg ccttggtagc atcggtctgg aatccatcgt
     5581 accaggtacc attctcatcc tgcaggattg cgggataagc atcctgccag aactcaggca
     5641 gatagtttac ataaggagcc tcgttaccaa tgaaaccagc tgcgataaca ccataggtat
     5701 caccggttat gccgtcgcca tcgggatctt cgttgtggaa ctttaacagc atgttgtagt
     5761 aatcatcata agtcttgatg tcttcagcct tcaggccaac tgcatctaac catgcctgct
     5821 taacgtaggt aacacatcca ttaccatagg taggagcgaa accatacaga tgaccttcct
     5881 catccttcag gttctcgttg atctcaggca ggatcaggtg agactggaat ttagcgtttg
     5941 catatgcttc ggacatatcc cacagcagac cggtaggtgc atactgcttg aacatatctg
     6001 cactcatgat cattacatca ggatagtcac cgcctgcgaa cagacgacct actgcatcgg
     6061 tataaccgga gtggtctaac tgctggatgt tcatcttaat aggatgaccg attgcttcgg
     6121 atactgcatc atcccactgc tgaacgaatt cagcctggcc gttatcaacg gttgcggtca
     6181 gagtaccatt aactactaca ttaatctcct ccagattgta agcttcggta gatgctgcct
     6241 cggtagaagc tgctgcgctg ccagttgctg cgctgtcggt ggatgctgca ggagcatcat
     6301 tgctgcttcc gcatgctgct aaggaagcta ccatagcaga tgctaaaagt acggataata
     6361 cttttctctt catttctttc ctccctaaaa atttggaatc atgatttgtc atggggtcat
     6421 tcccttgact tgccctcacc ttatcatcag ccatctggaa aaacaagtta caatttttgc
     6481 gttcgggtgc aaattttgac caccgattct gcaaaccctt gattttactg cattctttga
     6541 aagcacttac atttgatatt tttaaatttg aaaggggtta catcttgctt cctttgtaag
     6601 taactgatac aatagacatg gtttattcgg tatattatct aaaaaaattg agacacggga
     6661 gtgatcatga atgattttta ataaaaaaga cacccttaat gatacaaatt atacaagcca
     6721 caacaataca ttaatgctga aaatggtaac ctcttacagt attttcctgc tgatcattct
     6781 ggttttgttc atctttttat accgttctac gataaataac gcgcgtgatt cttatgatca
     6841 gcagaacgag acaaccctga tcagcaatgc agagctgttt gaaagcgatc tgaatatcat
     6901 ggaagtctat tgcagacagc ttctgcagaa cgacactttc cggaaagtca tgaattatga
     6961 gaatacctat tatcctttta cggaaatggg aaatgaattg cagaactctc tggctaccaa
     7021 tgtatatgca gaagctcttc ttccgttaaa agagtccttt gtctattttc cggagacaga
     7081 ctatgtattg aatcctactt attttatatc cgcaaagcgg ttctataact ggattcagaa
     7141 atactcttct accgaaaagg aactgtggca ctcctacatg acggaaccgg aatacaagaa
     7201 ccgctttctt cctatggatc agttcatgcc aaattacagt gaaaaatact atatgtatat
     7261 catcgacctg aacgatctct attatatgga tgccaatgcg aaggtctgct ttatcttcga
     7321 acaggataag atggcggatc tgttcgactg tgttcagatg gatggtgata atttcctggc
     7381 ggtgaccgcg gaagatgact ccaccgtgct tacgataaat actccgaaca gcatttccgc
     7441 agaaatgttg gattctctgg actttaataa gggctatgca gaggtccgga tcgatggaca
     7501 gaactttact ttggggaaat acgtttcgga taacaccagt tattcttact atttcagctt
     7561 cccgccctac aaggcggccg ccggattggg tggcacccag ttgcttttca tcttttttgc
     7621 cctgctggca ttgcttacag gcggctgtct ggtctattat ttctcccgcc gcaatgtccg
     7681 cccgatcatt gagcttgggc aggaattgca gaccgctgtg gaagtacaga aaaatctgca
     7741 ggaagtggta gacagtcagc gtccgatcat ctgtacctcc tatgtgagac agctgttatc
     7801 cggtactgct acttccgagg atgaagttac ctacatggaa aactatctgg gtattgcgga
     7861 ttcttcttat tataatgtgt tgtacatcgt ctactataat aatttcggag acaatacaga
     7921 aatcactctc cccggcggcg tggaatccat ggaagaactg gaaaagatca tcgagtccgc
     7981 actggagaat ttcttcggta caccgctctt ctacttcagc ccttcggacc ggacctattc
     8041 cgttctgctg cactgttccg aagaagaggg aacggatctg atcctgaaga tcaacaattg
     8101 tattttacga cttcatgatt atctgctgga tacttacgac atctggctct tcgccggtat
     8161 cggacgcaat acggattccc tgatgaatgt atgggaatgc taccagcagg cctccgaagc
     8221 tgtaagttat acgacgaaga actatatttt cttcccgtat gaatttatta agaaggattc
     8281 caacgtattc tattatccgc cggagatctc cacgaagctg atccacttca tctccacggg
     8341 caatacgccg caggtattag agatgttcaa cctgatccat caggagaata tcgaggaacg
     8401 taccctgccg gtcaacctgt taaaatacct actgtcagat atccgtaaca ctttattaaa
     8461 agcaagattt gcactgcccc aggacgcaga ttccgaagct gtaaaggtac tggatgaacg
     8521 tttcaatgag caccttacct tcaaattgtg cgaagatctt gccttatccc tgtgtaatct
     8581 cttcggcaat aaggaggatg acaatacgtt atccgccacc attgagaaat atattaagga
     8641 aaattacaag gacccttcct tagggcttaa taagatctcc gatgaattcc agatctcaga
     8701 gagctatttc tcccatatgt tcaaggaaaa gaccggagtc aacttctcca cctatctgga
     8761 gaatatccgt atgacagaag ccgcaaggct gatcaaggag accgatatca gtctgaatga
     8821 attgtatatt gctgtgggat ataataattc caatactttc cggcgtgcct tcaagaaggt
     8881 atacggagtg acccccagtt ccatgcggga aaaatagaac aatctcttga tagaaatgca
     8941 caaaggaaca gccgggacat cttcggagga tttaatcctc cagaaatgcc tcggctgttt
     9001 tcatatcttc cggcgttgtg atcttaatat tctgataaga agcctctacc agcttcactc
     9061 ttgtctcagt aaacagttcc accacgctgg catcatccgt tacctgaatg ccctgttcct
     9121 gcatcctggg aagacgattt ttcagtctct cgtatgcctg caggatcagg ctggtctcaa
     9181 atacctgcgg tgtctggata ttccagacgg ttcttcgatc cggagtggac agggcaaagc
     9241 cttctccgtc actgatcttt accgtatcct tgctctgtac cgccgccaca caggcctgat
     9301 attgcacggc cgctgcatag gtatcttcta atatcttctc tgtcagaaaa ggtctggctc
     9361 catcgtgaat gaacacatat ccgtgctcat tgggtacttt caaccgtccg tcagcaatca
     9421 gctgtaaggc tctccccacg gacagatacc gttccgctcc tccagaagta ataatattta
     9481 ctttggtaaa atgatatttt tgcacgattt cttcccggac atagggaata tcctgctctg
     9541 ccgtcaccag aatacagtca tcaatgatcc gggattcctc caccgcatgc aaagcatacc
     9601 agatcagggg ctttcctttt aacggtaaaa actgcttcgc caccgccgac tgcatgcggc
     9661 ttccgctgcc cgccgcaagg ataatagcgg tacatctggg cttttcccca ttctcctgag
     9721 gctcctgtcc gttttccatc tcatttttca tgctgtaatc ttctgtctca tttctgtcct
     9781 gtgctactct gtctaccata ttgtgtctgt ctttccttga agtctttttt cggttcttat
     9841 cgctcgccca gctttaaatt cggcaccgca ttcagatcat aaccgctgcg gacaccctta
     9901 atgtactcgt aatagccggc cacaccgatc atcgccgcat tatcggtaca gaaaatgggg
     9961 gagggatgat aaaatgctat gttacgtttt ccgcattctt cctcaaacgc tgcccgcagg
    10021 gaagaattcg aagccacgcc gccggcaatg gcaaatttat tcagtccata agccttcact
    10081 gcatggaggg aatggctcac cagcacatct acgactgctt tctggaagga agcagccaca
    10141 tccgcctgat taatctcttc tcccttcatc tggcatccgt tcagatagtt cagcactgcg
    10201 gatttcagac cgctgaagct gaaatcatat tcattctccg ccactgctgc tctggggaag
    10261 tggatggcat ccggattgcc ctccttcgac accttgtcaa tcttgggacc gccgggatat
    10321 cccagtccga tggcacgagc caccttgtca aaagcttctc ccgccgcatc atctctggtc
    10381 cgtccgatga tctcatattc accgtaatcc ttgaccacca ccagatgtga gtggccgccg
    10441 gacgccacca gacagataaa aggaggctcc agttctttat tctcaatgaa gttggcactg
    10501 atgtgtccct caatatgatg tactccaacc aggggaattc cggtagcaaa actgattgct
    10561 tttgcctcag atacacccac cagaagtgct cccaccaatc cgggaccata ggtcaccgca
    10621 atggcggtaa tgtcttccag cgtcacatgg gcatcggcaa gcgcctgctc aatgacctga
    10681 ttgatcttct cgatatgttt gcgggatgcg atttccggca ccacaccacc gtacagggta
    10741 tgtaatgcaa tctgtgagga aatgacgttg gaaagcactt ccctgccgtt tttcaccaca
    10801 gaagccgctg tctcatcaca ggagctctca attcccagaa tataaatatc tttttgtttt
    10861 tctgtctctg ccatttttct gcctactttg cttttaatta ttctcctgct gttccagact
    10921 gtcattcaac tgatccgccg tctcccagcc gtagatcctc catttgcggt tttcatccct
    10981 gcgaagcaga tataccagca tggtggaact cttttttccg ttttccgtca tgccatagcc
    11041 acaggcaatc cgggcaaaag aatatccgtc tgctgtataa tagtccacat tggtgctgga
    11101 cgcaagagaa acacttgtta tttttctctt attgtcctta tagttcttaa cctctccttg
    11161 taatcgggtc agataggtat cttcttcgtt atttgcccgg agttcctcat cgaacagctg
    11221 aagggatttc ctgcccagtt cctgaagctc ctccgtggta cagtcttcat tgtagaagca
    11281 tttgatcaga tcattgtaat atctgattac ttccttaggt gtcgcaggat aattattggt
    11341 cagatctctg gagagtgccg tatccacctc tgacataatg gcctcctgct gttctgtcct
    11401 gctttttccc gatagatacg aataatatcc cactaccagg acaatgagga agatcagaaa
    11461 aaccgtaatt ttcgtcatcg acgtgttgtt ttttttcata ttttcctact tcccttcatc
    11521 aaagttcaat tccatggtcc agccgtcctt tgccgcataa gctgcgggga aaatcttccg
    11581 gacatcatcc atgaattcct caggtctcgt catagaaggg ctgtaatgtg tcagccacag
    11641 tttcggtgcc cccacttctt ttgcgatctt cgccgcttcg tacattgtca tgtgcttatg
    11701 ttctctggct ttgaccagct tgtcgggttc gccatacatt ccttccagaa tcagcagatc
    11761 cgctccctgg gcattctgac ggatgctttc agtaggacgg gtgtctgtac tgtaggtcac
    11821 cttcaggccc tttcgttccg ctcccagtac catatccgga gtgaagactc ttccgttttc
    11881 ctccagagtc tccccctttt gcagacggct ccagaatttc atggggattt cctgctccaa
    11941 agctctgtcc ttatcaaatc gacccgcacg gtcaatcacc atactgtacc cataacagag
    12001 cacattgtgg ttcactttga aggcttccag acgaaatcct tcaaaagaaa atgtctgggt
    12061 attctccgtg atctccatgc attttacttc aaaaggaagc tccggtgcga tcacccgcag
    12121 agaagccacc actttggcaa gtcccctggg cccgatcagg agcagcggtt ccgtgcgctc
    12181 tgcatttccc atggtcagaa gcattcccgg aagaccgctg atatgatctg cgtgaaaatg
    12241 ggtaaagcag ataatgtcga tggggttcgg gctccagcct ttttccctca tggcaatctg
    12301 ggtgccctct ccgcagtcga tcagaatact tttcccgtta tagcgcatca tcagggaggt
    12361 cagccatctg taaggcagtg gcatcattcc tccggttccc aataagcaaa cgtctaacat
    12421 ctttcctcgc aatcttgcct aagcagcatc tttttgttct ttctgtctat aaaagctctg
    12481 tctccggcat ttcttccacc ggaacggcaa acgtatggat ccatttgtca tagcagtctt
    12541 cgcacaggtc aaacttgtgt ctgagcccgt ctttcctgct gaaatagcca aatctcacat
    12601 ctgcggcgaa acatccttca cggacgattt cattttctat tttcaactcc ttgccgcagc
    12661 cgttacagat tacctgctgt aatttaccgt tttcttcttt tcgcatcctg tttccacctt
    12721 tctgactgta aatgttacct tttgaccctg gcatcattat aatggataat ccgccggaaa
    12781 agtgatggta atacttgtta acggcgccac atgatcatgg catcctctgt cggtttttca
    12841 taaaatttcg ggcggatccc ctcggagaca aaaccgaatt tctcatacaa gtggatggcc
    12901 ggtgcattgc tcactctgac ttccagcgta tatgctgtga tcccgcgctt attgccgagg
    12961 cgtaacaact cttccagcat tttgccgcca atgcccagat ttcgatattc cggtgctgtc
    13021 accacgttgt cgatattgcc ctcatgaaaa ctgtccgtca gcccggcgca gcccgcgata
    13081 tgaccgtctg cctccgccac gacatacagg caataatctc tcgtcagaag tcctctgaaa
    13141 tcttccgcca accatggcat gctgaaggac gcagcttcga tccggctcag ttcttcgata
    13201 tccgtattct gtaacttacg aacttctatt ttcatgcctg tgcctctttt gcagccttct
    13261 cctgttcctc tcgttcccgc tctgcctggc tcttacgcag gtattcgggt tcatgctccg
    13321 ctgcggtcac cgttctgcca tgcgcatagt agacagctcc cagagaagcc acactggcag
    13381 cacgctgacg attattggca gccggagcaa aggagcaggg aaccttcagt ttctgtaaga
    13441 tctgttccct gtagaccgga acgccgtctc ctaagaagat cacttcccgt cccaaacggt
    13501 ttaattcctc caccagctcg tccacgacaa tagcgcactg ctctttgata ctgtgcatca
    13561 caagttgatg ttctgagctt tccttttccg ggatctcctc tttacagaac tcataaattc
    13621 cggtatatac ctgattcctt ctggcatcca tgatgggaca taccagcttg tccgttcccc
    13681 agaggttaaa agccagtccc tccagtgtcg ggacctctac cagcggaagg ttcagtgcca
    13741 gtcccagtcc ctttgccgta gccgccccga tccgaagtcc ggtaaatgaa ccggggcctg
    13801 cggaaatggc aatcgcatca atggtatgca gatccagatc gatcatctgc cggatctcat
    13861 ccagcatggg taataatgtc tgggaatgtg ttttcttgta atccgtggtg tattccgcga
    13921 tcaggacgtc atcctccacg atggcggcgc tggccaccag tccggaactg tccaaagcta
    13981 atattttcat actgtagtcc tctcctcaat cgtgatccgg cggtaatcaa agcctttctc
    14041 cagatccttt tctatggtca cctgcctgta atgttccggc aggatctctt cgatcagatt
    14101 cgcccactcg atcaggcaaa gtccttcccc gtaaaaacaa tcctcatagc caatctcctc
    14161 catctcctcc acatcgccaa tacgatagac atcaaaatgg taaaagggca tacgtccttc
    14221 ctcgtagatc tgcacaatgg taaaggtcgg gctgttcacc ggctccgtga ttccgaggcc
    14281 atctgccaca ccttgggtaa atacggtctt gcctactccc agatcaccga tcagcgtgta
    14341 gacctgtccg ggaagtgctt ccctgccgat ccgctgtccc agtgcaaagg tctcttccgc
    14401 actgcgggtc tctatgatct cttttttgca ttctctgtta tctgctgaat cgcctgttgt
    14461 cattttcctt acgctttcta ttgttacatg ttacgtgtta catgttacgg ttatgcagtt
    14521 actgttactt tttatttaat tcgtaactat tcagagccat gcaaaactgc gttctgcgaa
    14581 tgcctgcata cagacagata agcagtttta tatggcgaag taaccacatg ctctattctt
    14641 ttgctgtatc catgcatcta gctgcggatc ttcacttttg tgggcggcat atgggtggaa
    14701 atcatttcaa taacatccgt tgtcttatcc tgcatctgac ttttaggaaa aattgttgca
    14761 ttatttttcg ccgtatacac aatgatgctg cgtccggtgg ataccgcttt caagcaatcc
    14821 tcccatttct gacaggtgga gctgtccccc tgggagatgg taatgccgtt ctcatccaac
    14881 acataatgca gcggttcctt gaagaccgga ttgtttactg cctgctgttt gctcttgata
    14941 tagagtgtcc atgggagata taacagaatc accacgccaa gaatcagata gatccagtac
    15001 tgtctcgaga tgcccacgat cacgcccaaa gctccaatca ctgctcccag aagtccggac
    15061 acactgttgt agctgtgccg caataaaaag tcatataaat cccccgatgt aatcttcaca
    15121 tccaattcta ccataatgag ttctttcctt ataataatgt gactgcatag ttacttgcta
    15181 ttataatccc atccccacca tccggcgact gcgcatagga ttgcatacta tagagaacat
    15241 tataaatttt ttcccataaa aaagcaagta aagaaccgca gggattccaa tatctgtgac
    15301 ctgcggttct tatagtttac gttttgttta attatgattt aacttttatc acatattatt
    15361 catgccgtaa tgcttcgata gggcttaatt tcgctgcctt tttcgccgga taaattccaa
    15421 agaaaatacc tacagccgag gagaacatac tggctcccag caccgtaccc acactcacct
    15481 ttgcggtaaa tccaatcagg ctgcagattc cgaaggctcc tgccacaccg atcaggatac
    15541 cgatcaggcc gcccagcagg gtaatgatgg cggattccga cagaaactgt aacaggatgg
    15601 acccggtcct ggctcccaga gatttccgga taccgatctc tctggtacgc tctgtcacgg
    15661 ataccagcat aatgttcata acgccgatac cacccaccag cagggagatg gctgccacaa
    15721 ataccacgaa aatggtgatg tagcttaaga tctgatccat ctggctcata atatcattaa
    15781 agttctgtac taagatggcg ttctgtcctc tgacaccgtg gcggttctcc aacaatctca
    15841 ccacatcctt cgccacctga gaggcattgt ccgcaccatc actgacgatc agcagatccg
    15901 tatactccac atagaatcca tatccgtccg atatcgtgga gatgggtgcc tccatggtca
    15961 ctgtaccggc tcctgccatt ccgaaaagtt tcgatgcgtt gtccttacga ataccaacaa
    16021 tcgtaaattc ctgtgtcact ccatacaacg tataatcaaa actcattccg atcacattgg
    16081 tattaccaaa cagcgttttc gcgctgctct ctgtaatgac acagacttta ttcgccgtat
    16141 aataatccga atcggtaaaa taacgtccct tgatgatcgg atcctggctc gaatattcca
    16201 gtcccgcctt accgaaagtt gcagcagcat caaaagttcc ctttctgccg gtggcgcttc
    16261 cgctgaaact ccaggacggt gttaccgcct tgacatttgg aactttctcc agaatggcat
    16321 ccatatcctc ttccgtgaat accacctctg ttccgttttc cgcggtgtta ctactatata
    16381 ctgcgatctg gccgcccgcc atagaattca gttctccgtt gatcccactt ttcacgccat
    16441 tacctatgga aatgatcatg atcacagaag agattcctat gataatgccc agcatggtca
    16501 ggatcgaacg tcctttatta ctgcggatat tcatcaatgc aattttgatg tattcccata
    16561 cttgtgtcat ggatccgcct cctactgttc catgggcaca gccgttactg ccatgccctc
    16621 ctccaggctg atacccgcga ggctgttcac tatgatctgg tcttcctcag taataccctc
    16681 caggacctct gtgtattcat cggaggagat tccgcaggta attgccctct tgactaccat
    16741 gccgttctcg atcacataga ggaaatctcc atttttatcc gcattgatcg cttctaccgg
    16801 gatcaacaat gtatcctgtg cactgttggt atgtacggtc agcttagcat ccagtccgag
    16861 gatgatcttg tcatccgggt tggtcagatg tacttctacc cctaccatgg gcgtattgga
    16921 tgcgttcagt gttgccatac gattgatctt actgatctcg ccttcgtatt tattgccgga
    16981 gatggtaata tctactttct gcccgatggc aagttttgcc acatcggact tggaagcatc
    17041 aaaggaaacc ttcacattct ggctgctctc cagtgtgatc agctgtgctc cttctgttac
    17101 gctggctccg gagacaccgg tacactccgt tacaataccg tcaaagtccg caacaattcc
    17161 ttttttcgcc gcatcgtact gttcctgtgc ctcctgatag gtcagctgtg ccagatcctt
    17221 atctgccgca taggcttgcg actgatagcc attcaggatc gttccctcac tgctgccctt
    17281 ctggctctgc atctcggcct tgtaattttc acattccgta atatgctcct gcacggtagc
    17341 gatctcattc tgcatactca ctacataatc actggaatta gcaatagact gcacgtaaga
    17401 atttctggcc tgactgctgc tgatatcctg taattcctta ttgatttcct tggcccgttc
    17461 tgctgtcaca ccgtcctgca gttctttgtt taggtctgcc gcttttctgc tcagatcgta
    17521 gctctcctca gacaactgtc tctgggtctc cctctggctc gtactcagtt tatcctgcag
    17581 ttccttgaga tatgccttgt aatcggtaag ctgctgatcc aacactgcta gattggtatt
    17641 ggcctcattc agttttgctg tacttttgct gttctctgtc atagtactgt tataagaagc
    17701 attactctta tcctgctgta aggacgcctc ctgcaggcgc tgttccatct gatccatgtc
    17761 gtaggtcatc aggacatctc ctgccttgac ggcatctccg gctgccacat tgatctcgct
    17821 taatcttccg ctgaccggag caaataaaac ggtcttttcc tcactggcta cttttccgct
    17881 ggtgctgaca ttttcttcga tgtctccgcg aaatgcatta gtggtgctta ccatcactcc
    17941 agcattgccg gaaaacgcac agcttaccat gcgtatcacg atcagtacaa ttatgatagc
    18001 cgcaataata acaggtgctt ttttacgctt tttgcttggg ttctttgcct tctgttcctt
    18061 ttttgcttct ttttgctttc tcttttctgc tttcttcatt tccttgattt ttttagcact
    18121 cagtgcctgt tctgtttttt cctgctgttc cattatccct cacttatgat cctttctata
    18181 ttcattcata ctcttatctt ttcataattt gttagccgtg accggcggca tggcattgca
    18241 tgtgaaagat ttattttcca ctacgctcct gctgtctgca gctcctccac cggaacgatc
    18301 tgtccggaat cggattccac tttgccgtcc ttgatcttga tcacccgttt tgcctgtgcc
    18361 gcaatatcat tgtcatgggt gatcaggatg actgttcttc cctcattatg taaacctttc
    18421 agaatatcca tgatctcttt tgtggaattg gagtccagat tgccggtagg ttcatccgcc
    18481 aggatcaccg gtggtgcctg agcaatagct cgggcaatgg ccactctctg ctgttgtcct
    18541 cccgacatct ccgaaggctt atgatccata cggtgttcca gtcccacctt tttcagtgct
    18601 tctctggaaa gctcctgccg tacacttctg gatactcccc ggtagatcag tggcagttct
    18661 acattttcca ccgcagtcag attggggatc agattgaatc cctggaaaat aaagccgatc
    18721 tcccggttgc ggatatccga cagttcatcg tctgtcaatg cggatacatc ctgtccatgg
    18781 agccaatatt ctccactggt gggcacatcc aggcatccca gcatattcat cagcgtggac
    18841 ttaccgcttc cggaatgtcc gatgatggcc acaaattcct gttcgtcgat ggtcaggctc
    18901 acatgatcca atgcccgcac ctcattttcc ccgggattgt aaaccttgca cacatctttg
    18961 atctctacta atgcactcat tcctcacata ctcccatttc atgatacatt ttctgcgtat
    19021 tcttcattgt gttactgtac tgtttgcata atacaataat atcttttgag tgtcaacaca
    19081 tttctataag tatttgcaac tattcagagt catgcaaaag gaaagctctg gaagggcgat
    19141 tttgcgcatg cagttctgaa taattattac agctactata acagggaaat ccgaaaaaaa
    19201 tgttgggaaa attctaaaga ttttctatat tgacaatatg ttcacaaagt gttatattcc
    19261 atgcataagg taaagttctt cagggttggg tgcaattccc tactggcggt aataggtcat
    19321 ccttaagtcc gcgacccgca gatgcattct gcggctgatc cggtgtgaat ccggaaccaa
    19381 cagtacagtc tggatgaaag aagacgtatc acttatattt ttttgtgata cccatgcccc
    19441 ggaagcttgt attccggggc tttttttgaa gactttcctt gattttctca taggaaagga
    19501 gttctgaaat gaacaaatta atgcaaactg taactgaaaa tgtggcctat gtagtcatct
    19561 tcctagcggt catcgttggt tgtttcctgg tggctgtggt gctggaaaag agtgcccaga
    19621 agaaaaacgg agtttctgag ccgatcttca acaccagaaa gcttgccatg atcggtatgt
    19681 tttccgccat cgccatgatc ctgcatgtgc tggacttccc catctttttt gcccccggct
    19741 tctataaaat ggacttcagt gagctgccga tcttagtcgg ttcctttgct ttcggtcccg
    19801 tagcaggtgt catgatggaa ttcgtgaaga tactgctgaa gctgtgcata aaaggtac
//
LOCUS       DAWEJW010000054        19104 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1101_length_19104_cov_64.690167, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000054 DAWEJW010000000
VERSION     DAWEJW010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 19104)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19104)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19104
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1101_length_19104_cov_64.690167"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4711 a         5196 c         4085 g         5112 t
ORIGIN      
        1 agcggaagcg cgtctgcaac tgaaatatgc agttgctgca accgtacggc ttaaaatttg
       61 cttatgattt atttctgtac cagatgaact gcgaatccgc cgatctcttc cttcaggtag
      121 atagccttca ggttgccctt ggcatctctc ttagcggact cttcattgaa ctcaacaccc
      181 agaacagtgc tcaggtagtt gacagctctc tcgatgtagt tggtcttaac agcgatgtgg
      241 ccgttcttgc ccaggtaagg agtcttcata acttccagag cagagcctgc gaatacggag
      301 ctgttgccaa ccttagcggg cataccgaag atgaatgcga aacggtttgc agccttaaca
      361 gcctcttcct cgttctctgc attaacacca acatgagcca gttcgaagcc cagcatggtc
      421 tgtacagctt ctctggtcag ctgctcgatc ttgtcccact caccggcgtt gatcaggtcg
      481 ccgggaacca tccaggaacc accgcaagcg atgatcttgg ggaagtcaag gtaagaagtc
      541 aggttcttag cgtttacacc gccggtaggc atgaacttca tgtttacata aggagctgcc
      601 atagccttga tcttagccag accgccggac tgctcagcgg ggaagaactt aactacatcc
      661 aggccaagct cgatagcctg ctcgatgtcg ctggggctgg aagtaccggg agtgatagga
      721 atgttcttct ctacacaata cttaacggtt ctgggattta agccggggct tacgatgaac
      781 ttagcgcctg ctgctactgc tgcgtcaacc tgctctgcgt tcagaacggt acctgcacca
      841 acacacatgc tggggaagtt gtcggtcatg atcttgatag cttctgctgc tgcgctggta
      901 cggaaggtaa cctctgcaca gggaagtccg cctgcgcaaa gtgcctttgc cagaggaagt
      961 gcatctgctg cgttgtcaat ttttacaacc ggtacgatac cgattttgct gatttgctct
     1021 aatactgcgt tcataaataa cacctttctg tcttatagac taatagttat gtgaaaaatc
     1081 ttcgcaggag atcctgtcga aaatatttct gcaaaatgaa accaatactt ttatgccagt
     1141 acttccttca ctgcctcagc gatcttgtcg cacttgcagt ttgcaaagaa atactcctta
     1201 aacctggcac cggataaagc accctttacc agttcacgat ccagattctt caggatggta
     1261 accatatcgg tatgagttac ttcttttacc tgatccagga tcttcttgtt gcgctgctcg
     1321 ggaacaactc tctcaggggg atatccaccg ccgccgggag cacagaacag cttttcaaaa
     1381 gtatactgta agttcagttc accgccccag ccgaagttct cagcaaaagg aagtgctaca
     1441 cagttaccgt cgtttacctg agagaacagg taagcatcca gaggggactc aatgtgtcca
     1501 cacagaacct tggggaagga attgcatgca agcatggcac cttcgccggt tccgcatccg
     1561 gtgataacga aatccgctgc cccggagttc aaaagaactg ctgccagaat accgttctgt
     1621 acgtaggtca gggaattggg atcctctgcg ccgcacatac cgtagttgaa tacttcatga
     1681 cccatgggct ctactacttt tttaagagta gcgcagatca tttcattctt agcagcctga
     1741 ctgttttcat taatcaaagc tatcttcatg agatactcct tttccaaacg ccacaacagt
     1801 cagccgctga aattcttctg cgactgacct gatgtgaagt gtttattcta tttttgatat
     1861 cctgcgtaaa tgcacaagct ccatagctac tcactcgcaa attcgtgctt cgcactctgc
     1921 atccggctcg taaactcacc ggatatttac tcgtgagctg tgcaaggaaa acaaatgtcc
     1981 gctacgcggc cgcttgtttt ccttttgcac aagttctact ggggctgctt gccgatgtaa
     2041 gcaaggatac cgccatctac atacaggatg tgtccgttga ctgcattgga tgcctcagaa
     2101 gcaaggaata cagcggggcc ggtcagctct tcgggcttta accatctgtt agcaggggtc
     2161 ttagcgcaga tgaactggtc gaaaggatgt ctgctgccat cgggctggat ctcacgaaga
     2221 ggagccgtct gaggagtctc gatataaccg ggtccaaggc cgttacactg aatgttgtac
     2281 tggccgtact cggaacagat gtttctggtc agcatcttta agccgccctt agctgctgca
     2341 tatgcagata cagtctcacg acccagctcg gacatcatgg aacagatgtt gatgatctta
     2401 ccatgaccct tcttgatcat tgcagggatc actgctttcg atacgatgaa aggtgcattc
     2461 aggtctacgt cgattacctg acggaactga tctgcggtca tctcggtcat agggattctc
     2521 ttgatgatgc ctgcgttgtt aaccaggata tcgatggtgc ctacttcctt ctcgatctga
     2581 gctaccatag cattaactgc ttcttcgttg gtaacgtcgc atacatagcc gtgagccttg
     2641 atacctttct cagcgtatgc tgcgattccc ttgtctacca gctcctgctt gatatcgttg
     2701 aagcagatgg tagcaccgca ctcagcgtat gcggaagcga tagcgaaacc aataccgtaa
     2761 gatgcgccgg taaccagtgc aaccttacct tccagtgaga aattagtgaa ttcagacatt
     2821 gttttttctc cttttcttta taaaaactgt tattttagtt acatgttcca aaggatctta
     2881 tttcagatct ctgttgtcaa caccgtccat atcgtcgaag tcctgattct ctccgaccat
     2941 accccagata aaggtatatg ctctggaacc gcatccggaa tggatggacc agctggggga
     3001 gatcactgct tcttcgttac gcattacgat gtggcgggtc tcatcgggct cacccatata
     3061 gtgcataaca aaggcatcct caggcatatc gaaatacaga tatacttcca tacgtctgtc
     3121 atgtgtatga caaggcatgg tgttccatac actgccgggc tctaacttcg tcatacccat
     3181 ctccagctga cagctctcta cctgtccggg aaggatatac ttgcagatta ctctgtggtt
     3241 ggcatcctcc agagtcccca gttctacttt attctcatcc tttacaatga ccacgccctc
     3301 ttcgggagta ccttccggct tgatcagcac ggtcggataa gtcttatgtg cgggagcgga
     3361 attcagataa aatttagcgg gctgtttctc atcttcactt gcgaaaatga tctccttgga
     3421 gcccattcct atgtacatag cttccttgta acctaccttg tacactttgc catcaacggt
     3481 aatcatacct gctccgccga tattgatcac acccagttct cttctctgac agaaatactc
     3541 tgcacggagt tcatctccgg cggtcagagt gatagggctt acaggaactg ctgctcctgt
     3601 aatgattctg tcgatgtggc tgtacaccag cttgatctcg ccgggtacga acagattctg
     3661 gatcagaaac tcttctctca gacgctctgt ggtgtaatgc ttcacatctc ttggtgatac
     3721 tgctgttcta agttccacct tacgcctctc tttcctcaat gaccggtctg cgtaccgtca
     3781 ttgaatgata ccttttcatg aaagcactta cattatagca tatcattgta accatttcca
     3841 acacattttt actatttttt ataaaaattt tggaaggctg taactgcttt tctcttttgt
     3901 atgagttaag tatactagaa aagattctca aagtcttttc atattttaat ctaaacattg
     3961 caaattttga acttttggtg tttaataaaa gaaaaagcga aaaggatgaa agaaatggaa
     4021 gaacttacct cctgtaaaac agccatagat aactgtaaaa cctccgggac ttttgccatt
     4081 gcccacttat ataaagaaga aaaagccatg gacatgcaca tccatgactg ctacgagatt
     4141 tactattcca tctgcggcgg caaacagttt ctgatcgaca actgcttcta caccatcgcc
     4201 ccaggcgatc tctttatcat caaccagtac gaaagtcaca agctgaccca gatcgacaac
     4261 tccgtccacg aacgtatcgt tctgtcggtg gcgccggatt tcatgaagct gatctccacc
     4321 aaagagacgg atctgagctt ttgcttcacc caccgcagtg cccccttttc ccataagctt
     4381 tcgctcaaca aggaacagca gaaacgtttc ctctattata tcaacaaaat cacttccgcg
     4441 gagggctttg cccatgacat cacggaatac gccgctttca tggagctgat ggtcatgctc
     4501 aacacgctgt tcatccgcag tgcagagcag acggcggccg gggaaaccgt aacagttccc
     4561 gccgaataca aggacagtag ctaccgctac aaccatcagg tggacgatat tctggcttac
     4621 atcaaccaga acatctccca gcctattaca gtggaacagc tggccggaca gttctactta
     4681 agcgaatcct atatctgtcg gattttcaaa tccgccacag gcaccaccat taataaatac
     4741 atcaccgccc gccgcatcag tatcgccaag gcggttctga acgaaaacgg caatgttatg
     4801 gaagccttcg aacgcagcgg tttcacggac tacagcaatt tcttcaaagc cttcaccaaa
     4861 gccgtgggtg tttccccgaa aaaatacgca agcctgagtg tcagctaaac tgctgacgct
     4921 caggctcttc tttttacatt tcttaatcat tcggtctctg gtagaaccgg cccgccagat
     4981 tctccgtacg gatcatgttg acaaaggcct gcgggtccgt ctcccgcacc gtattcacaa
     5041 ctttgctcac ctcgtctcgt cccaccacgg aatacagcac gtcacattcc tgtttggaat
     5101 aacagccctc acccttgatc agggtggcat cgtgatgggt gatctccgca atagcttcgt
     5161 agacatcctc cggccgtttc gtcacgatga tcagtgtctg cttctggtag cgcttgtaca
     5221 atatatgcag tacctgggtg gaggtgtact ggaaaataat ggaatatagc gccttgtccc
     5281 agccaaacag tgctccggcg attccaagga tcaccacatt tcccgcaaaa atataattcc
     5341 aggaatcaat gctgtacttt tccgacagga agatcgcgat aaaatccgta ccgccgctgg
     5401 tggcattggc gaacagacac aggctgatgg ccagtgcatt gatcagtcca ccgaagatag
     5461 caatcagcag aatatcctcc gttaccggcc aggcgggcac catatccgtc aaaataccgc
     5521 tcaccacgat catcagacag gaataaccgg tgaacttttt tccgatgaat ttgaagctga
     5581 tgaccaccgg aaccgcattt aagataaagt tgatcaccga aaaaggaggc tcccagtgcc
     5641 acaacagcac gctgatctcc tggatcagtc gggtcagacc gttgaatcca ccggggatca
     5701 atcccccggc ccggacaaag cttttcaaat tgactgccat gataaaggat cccgccagga
     5761 tcaggcaggt acgccgcaag gtatcacaaa tactcttctt cgtcataatt cgctccttct
     5821 tacatccttc ctgcctcttc cttcacagaa ccttcctctg tgtgcgtttt ttccgcattc
     5881 ccgtcttcca tcgtttcgtc tgctgccttt tgcaaaggct cattctccgc acagtctttc
     5941 cttttcctgt cacacctctg acacaacacg catcccaaca ggatcagtac acttcccgca
     6001 ctcactaaaa taccgatatt ccgtccttcc ggtacatagt gcatctgtat cgtatgttct
     6061 cccgcttcca gatcaaatgc catgagcgca ttgccaaact gctcaggctc tgcattttct
     6121 ccgtcgatct gcaccgtcca gccctcctca taaggcacgg acaggatcag tctgccggct
     6181 tctttcagac ttaaggttcc ggaaatatgg gtgctgtcca tcgctacatt ttcaagatgt
     6241 tcttttgaca atatctccac tgcctgtgcc agcacttctt cattcagtac ataagcttcc
     6301 gccgatacct tcggggtatc gtccgtgtca tccccattgg tcagcgtcac gcgctctccc
     6361 tccaacagat atcccagata cagaatggat ccgtccttca gatcactgta atcctgcgtc
     6421 tccagcgttc ccccaagcac ctgaaccttt ttcgtccctg tggcattgat cctcgcgtaa
     6481 tagtagcctg cccggtccgc cgtaatacat acattatctc cgctagtctc actggtcgcc
     6541 cggtccagta agggctctgc gattcccaga tcctcggtca gctgattctg cactctcaca
     6601 ccggtgccga tctcgtccgt aacattccaa cccatcggtg ccacatagcc aaaaggcagc
     6661 gtatatttac aatgatataa ataaacatct ccgctgttat tcacgatctc atacagaccg
     6721 ttttcgtatt tatccgattc gccgaacata taatccacat tcaggagtgc cgacacgaaa
     6781 gctgtcgctc cgtcaaatcc gtaataaacc ttgctgtgac gcatacccag cattttatac
     6841 aggtccatga cgttggaatt catggtggag gaaaatacgg aagccgtagg gtatcccgta
     6901 agagtaccgt catttttcgt cttgcgggta aacttctcaa tacggaagaa gtcatccccc
     6961 tcctgttcct gcgtaagggc gtacaacgcc ttataatcct cctggtctcc cagataagcg
     7021 cttcgtccgg tagtgcccag actcgtatta aatgtattga tgctcagctc cgccactatg
     7081 cacgccagca ccagaatccc cagcacctgc ctcgtcagtt cgtcttttct cgtccggtac
     7141 agatacagaa gcacaccata gatcgtaacg aacaccagcg tcagcacttc aatacccgtg
     7201 tcgaaatcct cgctctccac gaatttctca caggcaagta aaaatatcac cgccgccaga
     7261 tacccataga tgatctgcct gggagaagtg ccctccacat ttcggaaagc atcatagcac
     7321 atgacaagga tcagaaaaat atatagaaaa gactgtctcg ccggcaggga atccggataa
     7381 ttcagaccgt gccataaaaa gtccaggaca ttggttccga agctgagcaa caaaaatccc
     7441 gccagtgcca gattgcagaa ccgttttctc actgatattt tttcattcag tgcatacatg
     7501 ggcaccagta aaaatactgc cacaccacag taaatgttcg gccagtgttc cagtcccctc
     7561 tccgtggtca cgcacaggca atgtctcgcc agcatatcaa acacggaaaa ataggactcc
     7621 agcgtaccgg ggaaatccga attgccaaaa tccgtccgta gaatagcaca gacctccgga
     7681 atcaacaatg ccgctgccag acctccggcc agcagggagg caaatgcaaa tcttccgatc
     7741 ctgccgaata cgatccgtgc tttcctgctc tgcccttccg tcatggtata gtattccgtg
     7801 atcaacaaca ttccaaaata cagcaccaga aagatacaga tcataatgga gatgtaataa
     7861 ttggtcagaa tacagagtcc cagagtcacg gtatacatga cccatttgcc ttctctgacc
     7921 agccgctctg ctcctaacag gatcaacggt agcaggatca ggcagtccag ccacatcaca
     7981 ttccagttgt aggccgccag atagccggac agagcatacg cagtggaaaa caaaagagcc
     8041 gccggatcct ccttgccgaa cactttccgc agatagatgc tgaaggtaag ccccataagg
     8101 ccgatccgca ggatcaccag ataactcatg aattccatga tcaggccttc cggcagcaaa
     8161 aataccagcc agttaaaagg acttgccaga taatagacat acagtgccag gaaattcgat
     8221 ccgatcccta cattgtagga atagaacagt ccctctcccg ctttgatctt gtgtacaaat
     8281 tcacttaaaa aaggcatata ctgatggtac atgtcggaga acagaaaact ccggtcacca
     8341 aaaggatata tgccccgtat gataaaaaga accagcataa taaatgcagg cacaaagaac
     8401 gcaagcacag agatccctct ctgcatcctg cgctcctcac tacttgttgc tgtcgtttta
     8461 ttgccgttca tattattttt ctcccacgat ctcttttctt accttccgta atcaaacagc
     8521 agataatact ccagcctccg gtaatcgttc aatgcttccc cacattcctt caccgtcgta
     8581 agagtgccgt ccgcctctct cacacctgct gcgctgatca ccgggtattc cttccgaagc
     8641 ccggtcagac tgctctgcag ggcaggcagc ggcagatcac agttctccag cacatccatg
     8701 gccagataat tggcactggt ctctccgtcc atctgctcct caatgtcaaa gttgctccag
     8761 atcacataag gtactttata ttttaacaga ttttcctcat ctgttaaagt ctccggattc
     8821 actttattat ttcttaagat cggattgctc acataggtcg taggctgatg atccccgaag
     8881 aatacgatca tggtatcctc ttccgcctga gagaagtaat cgatcagacc ctgcagggcg
     8941 gaatccgtct gttttaccag ggacagatac atggacaatg ctttggagtc aataccatcc
     9001 acggtaatat ccggggtaaa gttatggaat tcctcttcgt aaccgctgtg attctgcata
     9061 gttacgttaa agacaaacag aggctctccc gcttccttgt tctcatacag ttcaatgatc
     9121 ttggcaaagc tgctctcatc ggaaatatag ttacggatcc gtttcggatt cctgaaatcg
     9181 tcctgtgaca ggaactcatc aaatccaagc agcggataga ctctgtctct gtcccagccg
     9241 ctggcataat aaggatggat cgccacagta tgataatcca gttccttcag gtaggaagcc
     9301 agagaaggca tctccttctg cacatactgc tggtaagcca cgctgccctg cggtaaaaat
     9361 cctatagtgc tgccggtaag gaattcaaac tccgtattgg cagtattccc gcccagcacg
     9421 gaaacattca ggtaaccggt tctggtattc tctgccccct gttgtaagct gtgaataaag
     9481 ggcatatagt cttcgttggt ggtaaactct ccccggactg ccagatccga aaatgcctca
     9541 tccatgatca caatgatatt cggacgcttc acgctctccg ggtcttccac agctgctgca
     9601 agcccagcgg aatccttccc ggcctgctca taattgctgc cggtgccatc tgcagaataa
     9661 tctgcgggct tctccacatc cagatattcc atttccatca gaaatgccac gatattgccg
     9721 tctcttttat tcatgacggt gggggtaaac agcttatcgt ataatccaaa gctctgcaca
     9781 aagctctcac tctgtatcat gccggtataa ccatagatca aggcaatgct taacaggatc
     9841 agtgccgctc tctttgccac tcttcccggt gccttcatat gaaaacggct ctcgatcaac
     9901 aacaaaatca ggaatcccac aatgaccagt atcgtactgg tggacaattc atagttgaaa
     9961 ttgcccgcca cactggctgc tgtactgatg gaataaatat cccagggcat gatgggtgcg
    10021 gaacggaaat tcagcacata ataattcgcc agtcccgcca ccatgaaaaa ggccgtctgc
    10081 aacatcagag ccgcccggac atatttcacg attccgaata acagaagtgc ggtcagtaca
    10141 tagaacgcca tgttcaacag ctgggttttg aaatgcattg tcgtaaaagg attatgggta
    10201 tacatttcaa acagataaaa tgtcaccagc ggacatactg ccagcgcgat acaggaagga
    10261 agcttctccc tggtaattcc tatttttcga acggaatgac gcaaaaagcc tgtaatggca
    10321 gctgcgacac ttcgtattga ttttttctgt aaataatcca tcgcttaact ctccttacga
    10381 ttctgaagag attttatcgc aatgacagaa aaataacaat ttccgaattg cacacattta
    10441 ctaacattac aggcttaatt ttgttacttt ttacttatat tttacgtatt actacttgac
    10501 ttcgtatttt ccggcttcct tcaggcgcag cattgctctc gccatggatg cctgggatac
    10561 atgatactcc tggatgctct gcttctgacg cagctgttcc ttcgcacgct ccagggcagc
    10621 ctccgcacgg accctgtcga tatcctccgg ccgttctgcc gtatccacca gcatgatcac
    10681 acggttatga gaaatataga caaaccccac acctactaca accttggtcc agtcgtcatc
    10741 ctctttttcc ttaaaccgta cctcgccttc cttcgtggca atgaccatat cctcatgatg
    10801 ggacatgatg gcctgctcgc cgtccagagc cggtacgata atgatgccgc atttcccgtc
    10861 gaaaaatact ttatcactgg caatgacctt caagctgaag gtgttcatac gcacaccgcc
    10921 tttccttact gtgccatgag cttcttggcc ttctccactg cctcgtcaat ggttcctaca
    10981 ttgaagaatg cagcctcggg atactcatcc atctctccgt cgatgatcgc acggaatccg
    11041 cggatggtct ccttcacagg cacatactta ccgggtacac cggtgaagtt ctctgccaca
    11101 tggaagggct gggacaggaa acgctgaatc ttacgggcac gatatacggt gaccttatcc
    11161 tcatcggaca gctcttccat acccagaatg gcgatgatgt cctgcagttc cttgtacttc
    11221 tgcagcattg cctgtacctg gcgggcggtc tcgtaatgct ccttacctac cacatcctct
    11281 tccaaaatac gggaggtgga ttccaaagga tccacagctg ggtaaatacc ctgctccacg
    11341 atcttacggg acagaacggt ggtggcatcc agatgagcga atgtagttgc gggtgcaggg
    11401 tctgtcaggt catccgcagg cacatagaca gcctgtacgg aagtaacact acccttattc
    11461 gtagaagcaa tacgctcctg caattcaccg acttcggtag ccagagtggg ctgatatcct
    11521 actgcggaag gcatacggcc taacaatgcg gatacctcac tacctgcctg tacgaaacgg
    11581 aaaatgttat caatgaacag cagcacgttc tggtgctctt catcacggaa gtactctgcc
    11641 atggtcagtc cggtctcggc tacacgcata cgtgctccgg ggggctcgtt catctgaccg
    11701 aaaactagcg cagtcttctc cagaactcca gactccttca tctcactcca cagatcgtta
    11761 ccctcacggg aacgctctcc gacaccagtg aaaatagaat atccaccatg ctcggtagca
    11821 atattacgaa tcagctcctg aatcagcacg gtcttaccta caccggcacc gccgaacaga
    11881 ccgatcttac caccttttgc atagggagcc agcagatcga taaccttgat accggtctcc
    11941 agcatctcta ctacaggact ctgctcttcg aaggaaggtg gatctctgtg gatgacccaa
    12001 tgctcctctc cttccaggga ttctccaccg tcgatggtct ctcccaatac gttgaacaat
    12061 ctacccagtg tcttattacc taccggcacc tgaattcctt ttccggtagc aatgacatcc
    12121 atatccttgc aaagtccttc actggcaccc agcatgatgc agcgaacggt attgttgcca
    12181 atatgctgtg ccacttccat gaccagacgc ttgccttcac tgtatacttc cagggcatcc
    12241 ttgataaagg gaagatcatt gttctcgaac tctacgtcta caacaggtcc catgacctgg
    12301 acgatcttac catgattttt ttcctttacg ttactcaatt gttcaggacc tcctttcttg
    12361 cctttttctt ctttttctga gccttggcac cggcaatgac ctcagtgatc tcctgtgtga
    12421 tcatagcctg acgtacccgg ttgtactgaa tggacagatc atggatcatg gcggaagcat
    12481 tcttatttgc tgcctccatg gccatcatcc tggcgttctg ttcactgcag aacgattcta
    12541 ccagtgcacc gtatacatat cccatgacaa aatccggaac aatgttatca agcactgctt
    12601 tcggggacgg ggtcatgaca agcggatcct gatacatact cagatcctgt ccgtctttgg
    12661 taataaaccg ttcttttacg ataggtagca gacgcagcat ctgcggttcc gtcgtcacgc
    12721 tgtttaccat attggtataa ataatatata cctcatccag ctgctcttct tcgaactgct
    12781 ccaggatcgt ctctgtgatg atcctggcac gatgcagggt cgggttctgt gctgtataat
    12841 ggaactgctc agccaccttg attcctctgt gtccgaaata ctgacgtccc agctcaccca
    12901 cgacaaacag cttgtagctt cctgtctgtc cggctttctg catctgttct tctgccattt
    12961 tcagtacatt atggttatag gctccggcca gtcccttatc cgcggtgact actatcagtc
    13021 ccgtagtctt ctccccagcc ggtttgtcct tccgttcatc gaaatattta ttatcgatct
    13081 ccggtaaatg acggacaatt ctgctgacca tcgtctgcag agtatagaag taaggctctg
    13141 tctcttccag attcttctta gctttccgaa gctttgtgga agagatcata tacatggcat
    13201 tggtgatctt catggtatcc tgaatgcttt tcatacgact ttgtatttct ctcgtatttg
    13261 ccatgttaac ctcctgttat ttgtaactat tcagagccaa tgggcgaagt aaccacataa
    13321 gcaaaagtaa agccctgaaa gagcggtttt gcgaatgggt ttctgaatag ttacattatt
    13381 tctgtaaaaa ttccttttta aattctgttg cagcatcaca gatctgcttg ttcagctcgt
    13441 catccagagc cttggtggtc tctaactgac gaacgatgtc gctgtgctgt tccttcatgt
    13501 agtctaagta cttcatctgg aaatccttta cctgacttac aggcacatcc agcatcactc
    13561 tgccgttggc tgcacacagg gtcataacct gctctgccat gctcaaaggt ctgcacagag
    13621 gctgcttcaa cagttccatc agtacatgtc cgtgctgtaa ctgttccttt gtggcgttat
    13681 ccagatcaga tgcgaactgg gtgaacactt ccatctcgcg gaactgtgcc aggtcgatac
    13741 ggatacttcc ggctgctttc ttcattgcct tggtctgtgc agcaccgccg acacgggata
    13801 cggacagacc gacattgact gcaggtctca tgccggagaa aaacagatca ctctccagga
    13861 agatctgacc atcggtaatg gagataacgt tggtcggaat gtatgcggat acatcgcctg
    13921 cctgggtctc aatgatcggc agtgcggtga tggagccgcc gcccttatcg gcactcagtc
    13981 ttgcggaacg ctccagcagt ctggaatgca gatagaatac atctccggga tatgcctcac
    14041 gtccgggaga acgttccagc agcagggaca gtgcacgata agctaccgca tgcttggaca
    14101 gatcatcgta tacgatgagg acatccttgc cctgatacat aaagtactca gccagtgcgg
    14161 taccggaata aggtgcgatg tactgcagag gtgcaggatc gctggcggtt gcagatacga
    14221 taatggtgta atccatggca tcgttctttt tcagggttcc taccagtttt gcaatggtgg
    14281 atgccttctg gccgatagcc acatagatac ataccacatc cttgcccttc tggttcaaaa
    14341 ttgcatcgat ggcaatggaa gtcttaccgg tctgacggtc accgatgatc agctcacgct
    14401 gtccacgacc gatggggaac atggaatcga tagccagaat accagtctcc ataggcacgc
    14461 ctacggactg tctgtccacg atggaaggtg ccggctgctc gatgggacga tagctttctt
    14521 ccttaatagg acctgcaccg tcaatgggtg ctcccagagc atccacgatt ctgcctttga
    14581 atccgtcacc tacaggaata ccggctctct taccggtacg aacgactctg gtgccttcgc
    14641 ggatctcggt atcatgaccg aacaggatac atccgatctc atctctgcgg acatcctgta
    14701 ccataccttt gacaccggaa tcaaagataa cgatctcgcc gtactctgca tggtcgatac
    14761 cattgacgtt ggcgataccg tcacctaccc agctgacaga accgatctca ttttcctgtg
    14821 cctgtaagtt gaaatcctcg atctcacctt tcagaatgga aatgatgccc tgctcactgg
    14881 cggtctttcc tacctgggaa agcttgtctg cgaactgctt catacgtcca ttggtactcc
    14941 agtcatagac ctcgttaccg gcacggatga tgaaaccgcc gccaagagaa gaatcttctt
    15001 tggtctcaaa gaccatatcc tctctgttgt atttcttctg gacaaaatta cggatattct
    15061 gcagctgctc atcggtgggc gctgtcacat aggacagggt tacaacgaac tcttcctttt
    15121 tctcgggaga gacttctctg tatgcggtgc agatatcatc ccacagatag acgtctccgt
    15181 tgtcacacag gactttcaga aaatctctga tctgtaaggg gaatactttt tctattacat
    15241 tgtgcttggc agacagcgac acagcagggg attcgaaatc cagcttcacc tgcggtacgg
    15301 ctttgaagat atcgcctgtc tgacctacaa tgtctccggg gatattcagt tcacgcaatc
    15361 tttttgcgta ttctaaagct gtactaatca atcttttcac ctgtctttgc tataaattgg
    15421 ttgtaaagtt ctctgtctga atctgcgttc tgcttggcag ctaccagttt tgcagtagct
    15481 tccactacca ggccggcgat ctgttcctgt gcctgctcga tgcgcttctg ctcttccagt
    15541 gtggcattct tactggcatt gtccatgatc tttttcgctt ctgtctgggc atctgccacg
    15601 atgcggtcat attcgttgct ggccttgcct cttgcctcgt tcagaatgct ttctttttct
    15661 gcctgaacac cgcttaagga tgcctcgtac tggcctttca gttcattggc actgtcctgt
    15721 gcagcttttg catctgcata ttgcttatcg atctctgcct gtcttgcatc caggatcttg
    15781 tggatgggct tgatcaggaa gatcttcaca atggcaaaga tgatcaacag attcaggatg
    15841 gtccatacaa tattaatatc taatctcagc atgtgctttc atcctttctt acgtttcgct
    15901 ttctaatttc cttataacta tccgaaactt ccattcgcaa aacgctcttt cagagctttc
    15961 tttttgctca tgaagtttta gtcatcgtaa atcgtcacaa aacagcttac aaatgcaggc
    16021 atttgacgct tgtctgtgac gcatccgact tcggatagtt atgccatgaa cagaatgatc
    16081 aggataccga taacgaatcc gtaaatagcg gttgcctctg ccagcgcacc acctaacagt
    16141 aagatcttgg tgatcttgct ctctgcttcc ggctgtcttg cgactgcctc tgctgcttta
    16201 cttgttgcga taccgatacc gatacctgct ccgatacatg ccagtgctgc aatacctgct
    16261 ccaatagcta tcataattct tttcctccgt ttttgtgtga attagtgttt cataactatt
    16321 cataatcgca ttcgcaaaat agctctgaat agttatccag ttactcaatg gcttccttaa
    16381 tgtacatgga tgtcaggaat acaaatacat atgcctgaat caatccatca aaaatatcaa
    16441 agtaaaagct gaagggtacc ggcaatgcta ccggaaccag aagcttcaac agctccatga
    16501 taacgaatgc tcccaaaacg ttaccaaaca atcgcataca cagggacagg ggcttgatga
    16561 acacctccag gatattgata ggtgttacga tcgcgatggg ttcggtaaag ctcttgatcc
    16621 atccgcccac accttttttg cggatacctg cggcttccac cagaacgata ctcatcaggg
    16681 ccagtgccgc cgttacgttc agatccttgg tagggggctt cataccgaac aatccaatga
    16741 tattggatat gcccagatac aacagtacgc taaccagata cggtacatac cgtttgcctt
    16801 cttctccgat cattcccgta acaatgcctt ccagctttgt cacgatcgtc tctgccacca
    16861 gctgtcgtct gctgacattg tgtactttga ggttgcttgt aaggataatc gacaagatca
    16921 gaatgactgc catgaccacc caggtgataa cgaccgattc cgcaatgtcc agctgtactc
    16981 caccgatcag gggtagacct gccggaagct taatagaaaa agccgtgtca caattcagtt
    17041 ccttgagcag ttctgatgcc aattcctcca taataccact tcctttccgt gaacatcctt
    17101 gaaagagttt tcactccctc acggtcgttg ctattttact cctatttatt gaaaaacgta
    17161 agatacattt ttgctaattt gttccatgca aagatgaaaa gttatggcat atattaacta
    17221 taaaaaatga ggtatcagaa aatcatatga caaaattagt cattttggga cgcaaatccc
    17281 agacgggcaa ttatgagacg tttttttcga aacttcccgc cagaaccgta accacactgg
    17341 tccccgggga agtcacctcc tgtgatgccc tgattctgcc gggcggcgga gatatcactc
    17401 cggctttctt cggagaaaaa aacaaaggtt cccgtactgt tgacaccgaa ctggacatcc
    17461 tgcaatttca ggcccttgac acagccattg ccgccaggat ccccgtgatc gggatctgca
    17521 agggcatgca ggtcatcaat gttgccttcg gtggcactat tttgcaggat atgtcgggag
    17581 attccctgca cagtatccct tccggtgatg cattccatac gtccattatg acggaagatt
    17641 cccgtctttt caagtatttt cctgtcaaaa tgcacgtcaa cagcgcccac catcagagga
    17701 tcgaccgctt aggtcagcat ctgaagatca ttcagcgctg ccccgatgac ggctgccccg
    17761 aagccttgga gcatgacgag ctgccgatcc ttgggctgca gtggcatccg gagcgtcttc
    17821 cggaacaggc cggtgagcct ctgctttctc ttctttcttc ttatttctga atctgcgccc
    17881 gggctttttc gccggggcca gctcctctct gaccttgact ttggcgattt cgaacaggga
    17941 gcggatgatt ccccgcagca tctccgcggt ctggtcgtcc acttccttca gtgcagtaac
    18001 caccgcattc atactctttt tccagtcagc ggtaaacgtg atcagatcat ccgcctggga
    18061 tgcggagatg acctgataga gtgtttccac gaaggtgcgg atctgttctt catttaagga
    18121 cagtatccat tcgttcagtg cttcattcat gatcttctgg ctctcataaa tgtcacccac
    18181 atccacgaaa tggtcctcct ccaccagcca ggaaaaagga tcgtgctgca aaagcccatg
    18241 gctgttgctc tccaccacgc ggtaatgaat atcccgctcg aaaatcatac cgatcatgga
    18301 agaatgtggc aacagcttca ccaccttgcc ttcgatggca ttatagtgac actccttcag
    18361 cacttccggc cggaatccag gcccatccag actgtagatc ttctggatcc gtttctgcac
    18421 aaaaggggcg catttcatgc cactgtagac tgccagattg ccgcccttgg aatgccctcc
    18481 gatataaaag ggctggtgta gccatccggt gaccatattt acatatttta cactgtactc
    18541 ctgccccggc accggtgaca gaaatgccat gttgaaatct tccttccagc ctatgatagt
    18601 ctcatccgtc ccccggaatg ccagaaacag ggttccgtca tccagaataa aagtgatggc
    18661 ggagaactgg gtctcccatt ccttttccac cagattgata tagcagttca gcttcatatc
    18721 ccggaaacgt ctgccgctta acattccttc gaacagcgcc cggttctcct tctcaaaacg
    18781 gacatccccg aacagtttgt ccacgtccgg acgctctgca atctcccgca gggtcacgga
    18841 agggccattg tgccgcacat cgctgaccat cccgtcgaat ttcagataag agagctggca
    18901 caatgccaga ctgtccacat cattcagagg tttttcccga aaagaaatgt ctccgtattt
    18961 ttgcaaatat tctattaccg ttcctgccat atgccgctcc tttccgcgag gaactttcct
    19021 tgcgtatttc gccggattgt gttacgttct atataatgat gccagggtca aaaggtctaa
    19081 gcccacatga gcttgctcat aggt
//
LOCUS       DAWEJW010000055        19039 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1107_length_19039_cov_52.570217, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000055 DAWEJW010000000
VERSION     DAWEJW010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 19039)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19039)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19039
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1107_length_19039_cov_52.570217"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5582 a         3755 c         5344 g         4358 t
ORIGIN      
        1 tcaatttaca taaaaaggtg taaagaaaaa atacttgaca cctatacttt tctatagtat
       61 gatagccaag gtgcatatcg catgacctat agaaaggcac tggataagat ggacaggatt
      121 ttggaacaga atctgttgta tgacttctac ggtgaacttc tcaccgagca tcagcagaga
      181 gtgtatgagg atgcagtata taatgatatg tctctcagtg agatcgcggg agaacagggc
      241 atcagcagac agggcgtcca tgatctgatc aagcgttgcg ataagatcct gcaggattat
      301 gaaagcaagc ttcatctggt ggaacgtttc atggcggcca aggagaagat cggtgagatc
      361 gaacagctga cggcagagaa tgatacggat cccggagaac ggatggaacg catccgggaa
      421 ttatccagat cccttttaaa ggaatggtaa ggagcagcaa atggcatttg aaagtttaac
      481 agacaaactc caaaatgttt ttaaaaactt aagaagcaag ggccgcctga cggaagagga
      541 tgtcaagtct gcactgaagg aagtcaagat ggcactgttg gaggccgatg ttaacttcaa
      601 ggtagtcaag cagttcgtga aggcagtgga agaacgtgcc attggccagg acgtcatgaa
      661 cggtctgaat cccggacaga tggtgatcaa gatcgtaaat gaagagatgg taaatctcat
      721 gggcagtgag accacagaga ttgccatgca gcccggcaaa gccattaccg ttatcatgat
      781 ggcaggtctt cagggtgcag gtaagaccac cgcaacggcc aagatcgccg gtaagctgaa
      841 gctcaagggc aagaagccct tgctggtagc ctgcgatatt taccgtcccg cagccattga
      901 acagttacag atcaacggta agaagcagga agttgatgtg ttctccatgg gaacggatca
      961 taaacctgcc gagattgcga aggaagccat tgcgtatgcg gagaaaaacg gtcataatgt
     1021 agtgatcctc gatactgccg gacgacttca tgtggatgaa gacatgatgg cggaattaca
     1081 ggagatcaag gagaccgtga ccgtgcatca gacacttttg gtagtcgatg ccatgaccgg
     1141 tcaggatgcc gtaaatgtgg caaaagaatt tgatgaaaag gtaggcgtcg acggtgtgat
     1201 cctgtccaag atggacggcg ataccagagg cggtgcagca ctttccatca aagcagtcac
     1261 cggaaagccg atcctttttg taagtatggg agagaaattg tccgatttag agcagttcta
     1321 tcccgatcgt atggcaagcc gtatccttgg tatgggtgat gtgttatccc tcattgacaa
     1381 ggcacaggcc agcatcgata tcgatgagga gaagagccgt gatatggccg gccgcatgaa
     1441 gaaaggtaag ttcgattttg aggattacct ggagagcatg aagcagatgc gtaatatggg
     1501 cggtctgtcc agcatcttag ggatgcttcc cggaatgggt atcaaggcat cggatctgga
     1561 aggtgccatt gatgagacgc agatgaagcg tacggaagcc atcgtattat ccatgacaaa
     1621 agaggaacgc aggaatccca agatcctgac ccctcagaga aaacaccgta ttgcaaaagg
     1681 tgccggtgtg gatattgcaa cggtgaaccg ttttatcaaa cagttcgagc agacacagaa
     1741 gatgatgaag cagtttggcg gcggcaaaaa gggccgcggc ggcatgggtg gtatgttcgg
     1801 cggcggaaaa tttcccggta ttggcggcag atttttctag cactgtttac ttaacttaag
     1861 tccgatatga acaatcagtt cttatcatat aaattatttt atttttaatt aatggaggaa
     1921 aaagaaatgg cagtaaagat cagattaaga agaatgggac agaagaaggc tcctttttac
     1981 agaattatcg tagcagattc ccgttccccc agagacggta gattcatcga ggagatcggt
     2041 acctacgatc cttccaccga tccttccact ttcaagatca atgaagagct ggctaagaag
     2101 tggttacaga acggagctca gcctaccgag caggttgcta agattttcaa ggttgcagga
     2161 atcgagaaat aagttctaaa tatctattca tcggaggtgg attatgaagg aactagtaga
     2221 agtagttgca aaggcactcg ttgataatcc ggatgaagta gttgtaactg agaagacaga
     2281 aggaaaaaat atcgtccttg aacttcatgt agctgcgtcc gatatgggta aggtgatcgg
     2341 taagcaggga cgtattgcaa aagcaatccg ctccgttgtg aaagcagcat catccaaaga
     2401 caacacaaga gtggacgttg agatcgttta atttcgaatt gagagatccc tgacgttgag
     2461 cggagaaaca tacaggcgtg tgtttctccg ttttttggct aaagccggaa tggagtaaaa
     2521 ctatatggaa gatctgttgc aggtcggcat tatcacttcc acccacgggg tgagaggaga
     2581 agtaaaggta tatcctacca cggatgatcc cagaagattc cgcagattaa aggaagtcgt
     2641 tctggatacc ggcaaggaaa aaatgaatct ggaaattgag ggtgtcaagt tctttaagca
     2701 gtttgtgatt ttgaaattta aagggctgga caacattaat gatatagaaa aatatcgtca
     2761 gaagagcctg tacgtgaccc gcaaaaatgc agtgcgtctg caaagggacg agtatttcat
     2821 cgccgatctg atcggactga aagtgcagga tgaagacggt aaggaactgg gcacggtaaa
     2881 agatgtgatc gagaccggtg ccaatgatgt ctacgaggtg gaaatggcag acggtaagag
     2941 cctgcttctg cccgctatca aacagtgtat cctgaatgtg gacgtggaaa atggcacaat
     3001 gcaggtacat gtactggaag gactgcttga cttatgatga agtttcatat tctgaccctg
     3061 ttccccgaaa tggtgcagca ggggctggca accagtattt taggccgggc agcggagaag
     3121 gatctgattt ccatagatgc ggtgaatatc cgggattaca cacaggataa gcacggaaaa
     3181 gtggatgatt acacctatgg cggcggggcc ggaatgctga tgcaggcaca gccggtatat
     3241 gatgcttacc gggtggtggc aggagacaga aaggtacgct gcgtctatct gacaccacag
     3301 ggagcccctt ttacccagaa aaaagcaaga gaactgtccg gggaagagga actggtattg
     3361 ctctgcggac attatgaggg aatcgatgag agagttctgg aagaggtcgt gacggattat
     3421 atttctatcg gcgattatgt cctcaccggt ggggaactgg cagccatggt cgtggtggac
     3481 gcagtggcca gactggtgcc cggggtactc aataacgacg aatctgccga gacagagagc
     3541 ttccataacg atctgctgga atatccccag tattcccgcc cggaggaatg gcacggaaag
     3601 aaagtaccgg aggtacttct gtcgggcaat cataaaaaga tcaatgcctg gcggctggaa
     3661 cagtccgaga gacggacgga agaacgccgc ccggatctct atgcaaaata tcaggaaaaa
     3721 cagaaagtca taaaaaaact ttccgcgaag aaacgtattt ttattcatat gatggagaca
     3781 ctgtccagag gattgggaga agttctctat gcggagggga aaaatgtact gatctatctg
     3841 ccggagatcg gcaatgccat gctgaatgcg gaggatgagg agcatttgga gaaaatgctg
     3901 ccgctcattc cgaaagccgt gtcggggcat agtatcgtga ccgtgacaga ccggtggaat
     3961 gaacgtgtca gtgaaatttt agggtatcat ggcagtatgc tatgttccca ggcgtgctat
     4021 accagagggg aaccacttcc ggtgaggcac aaggacatcc ggcagctgac agtggaagag
     4081 gttccttatg tggcggagca ttatcacctg ggagacgaga tttatgtccg ggagaggatt
     4141 acagcaggag atgtcttcgg catctatatc gagggaaaac tctgtggttt tatcggatgc
     4201 cacaatgacg gcagcatggg aatgctgtat gtggaggatg cataccgcag acaaggactt
     4261 gcagcatccc tggaaggtta tctgatcaac aaacagagag aacaggggat gatcccctac
     4321 gcccatattg tgaatggaaa tgaagcgtcc atacaactgc aggaacggtt ggggctgaat
     4381 ctgtcggatc ccgcgatctg gtggctgtat aattaaaaaa gcttgctttt tatatggcct
     4441 tatggtaaaa tctgaatgtt gtgaaaacga gatggtcctc tgttaccaat aagggtatta
     4501 agaacatcca ttttatgaag gaggctcatt atgagtaaca ttatcaaaga gatcgaagca
     4561 gaacagttaa aagcaaccgt tgatcagttt gaagtcggcg ataccgttaa ggtatacggc
     4621 aagatcaaag agggtaaccg tgagagaatc caggtattcg aaggcgttgt actgaagaga
     4681 cagggcggca gcaacagaga gactttcact gttagaaaga cttccaacgg tatcggcgta
     4741 gagaagactt ggcctttaca ttctcccaac gtagagaaga tcgaagttgt aagaaaaggt
     4801 aaagtaagac gtgctaagct gaattactta agagatcgtg ttggtaagaa ggcaaaggtt
     4861 aaggaattag ttaaataatt acattgaccg gaaaggggca gctgtttctg acagctgcct
     4921 tttatattta tttgaaaaag gaatacggat atggtacact gagacttgta aacactatgc
     4981 gagataggac agaacattga gaaataaggg actaagtttt tataaaaaaa agaaaaagat
     5041 cagtcaggaa ctgctcaggg aaattttcag ctggatcttc ggcattgcac tgtcagtctt
     5101 tctggctgca gtggtggtta tctttctggg aaagtccacc agagttgtgg gcatgtccat
     5161 ggaaccgaca ctggagaatg gacagcagat atttattgac cgttttctgt atgttttatc
     5221 ttctccgaag gcgggagacg tagtagcatt tctgccgaat ggcaatgaaa attcccatta
     5281 ttatgtcaag cgtgttgtgg cagggccggg agacaaggta aggatcgcgg acggaactct
     5341 gtatgtcaac gggcaggaaa gcaaatgggt gacggaacgg atcctggatg cgggtatagc
     5401 ggaaaacgag attatccttg ggaacaagga gtatttctgc atcggggaca acccgaataa
     5461 cagcgaagac agcagatccg ccaatatcgg accggtggag gagggcgata tgatcggcaa
     5521 agcctggttc cacttaaaat ccgggacgga tggcatcggc tttatcaaat agaaagtatg
     5581 gtaggagaat agaaaaatga attatcagtg gtatcccggt catatgacca aagcaaggcg
     5641 tatgatgcag gaaaatatca agctgataga tctggtgatt gaacttgtag atgccagaat
     5701 ccccatcagt agcagaaatc cggatatcga tgaactggga aaaggtaaat cccgtatcat
     5761 tttgttgaat aaatcagatc tggcagatcc ggtgtggaat aagaaatggg tcgagtattt
     5821 ttcaaatcag ggaatgggtg tactggaaat caattcccgt accggtatgg gaatcaagtc
     5881 gatccagggg ctggtacagg aagtctgcaa ggagaagatt gaacgtgaca gaaagcgagg
     5941 cattgtaaat cgaccggtac gtgctatggt agtgggaatc cccaatgtgg gaaaatccac
     6001 ttttatcaat tccttcgcgg gaaaagcctg tgccaagacg ggaaataaac ccggtgtgac
     6061 caagggaaaa cagtggatcc gtctgaataa aggactggaa cttctggata cccccggtat
     6121 cctgtggccc aaattcgagg accagcaggt aggaatgcgt ctggcattta tcggatccat
     6181 gaacgatgag atcctaattc cggatgaact ggcctgtgat ctgatcggag ccatcaaaga
     6241 gctttatccg aaggctttgc aggaacgcta cgaggcagat cctgcgggca agcccataga
     6301 gatactggaa gcagtggcag agagccgcaa atgctatgca aagggagaac aactggatct
     6361 ggggaaagcc gccggcatcc tgatcgatga tttccggagc ggcaagctgg gacgaataac
     6421 actggagagg atatagacag atgaacagaa tcatgagaga attattaaac acagcgatct
     6481 acctgctctg tgtactggga gcggtatggc tggtgatcac ctttgtagga cagcgtacgg
     6541 aagtggaggg agcctccatg gagaataccc tgcacaacgg ggataatctg atcgtggata
     6601 agttaagcta tcgttttcat gatccggaac gctttgacat cattgtcttc ccgtttcaat
     6661 tccaggacaa cacctattat atcaagcgta tcatcggact gccgggagag acggtacaga
     6721 tcatggatga cggcagtatc tacatcaacg gggaaaagct ggaagagaac tacggcatgg
     6781 aagtcataaa accggagacc atcggtcggg ctgcggaacc cattgaactg ggggatgatg
     6841 aatattttgt catgggagac aaccgcaaca attccagcga cagtcgtacg gatatggtgg
     6901 gtaacataaa aagagaaaat atcatcggaa aagcctggct ccggatctgg ccggtttctg
     6961 atttcggtat attacaacat caataaccat aaatggggat aaacaaatgg aaagtgcagc
     7021 aaagatcaag gaaatattac aggcagcttc aatggaaaag ctgcctgaat ttatagcaga
     7081 atatgagaat gatcccagaa gcggtgtgca gaagctggtg tctggcgcac tgaaaaagat
     7141 agatgctctg gaaaaggaac gtcagcggat cgagaatctg aaaaaatacg agaaagaata
     7201 cgcacaatat acctatatct gcggaattga cgaagtggga agaggtcctc tggcgggacc
     7261 ggtagtggca ggtgctgtca ttctgccgaa ggattgtgat attttataca ttaatgattc
     7321 caagcagtta tccgaaaaaa agagagaaga gctgtacgat gtgatcacga aggaagcggt
     7381 ggcctgggca gtgggctatg cttctccgga gcggatcgat gagatcaata ttttacaggc
     7441 aacctatgag gccatgcggg aggctgtcgg caaactggat ccggcaccgg atctgttgtt
     7501 gaacgatgct gtgacgattc ccggagtgga gatccgacag gtgccgatca tcaagggcga
     7561 tgccaagagc atttctatcg gtgcggccag tattgtggcc aaggtgacca gggaccgtct
     7621 gatggagcag tatgccgatg tgtttccgga gtatgatttt gcatccaaca aagggtacgg
     7681 cagtgcggca catattgcgg cactgaagga gtatggaccg actccgatcc acagacacag
     7741 ctttatcaaa aatttcggat tttaaggcat ttagcgtgcc gggagagaaa atatgaagct
     7801 gacaggatgg ttctcacagg ataaaatagc ggataacagc agagtacaga ctccaaataa
     7861 tgcgaatacc gaacaggtaa accggcagat ccgtgcactg gttcccgggc agaccctcag
     7921 aggagaggtg gtctccaggg agggcaataa tgcgcagatc cgtctgctgc aggatgtcct
     7981 ggtggatgca aaagtggatg cggatatccg tctggaactg gggcagaaca ttacctttca
     8041 ggtaaaaaat aacgggcaga cactgaatct gagtcctctt tttaccaata tggcaacaga
     8101 agggaccgta ctgaaagcgt tggatatggc atcgctgccg gtgaacgaga atacggtagc
     8161 catgacaaaa caactgatgg atgcggggct tcctattgat aagaatacgt tgcagcagat
     8221 ctggcatgaa agtaatgcgt tcccggatgc ggagattctg gatctcgtga atcttcacaa
     8281 agtggaactt ccggtgacgg aggaaaatat cacacagatg gcgtcctatc gcaatctgac
     8341 acatcagctg acagcaggaa tcgcagagac cggagaaagt ctgacaaata tgctgcaggg
     8401 gctggtagag agcggagaca tagagcaggc cgccacaatc tacagtgagg ttctggaact
     8461 gttggctttc gaagatgcgg ccggagagac ggtaacagga caacaacaga ccgagggacc
     8521 tctgccggag cctgacgtgg atgtgacagt gaccccggaa gaggcagaac agatgccagt
     8581 acagccttcg gcaacagcac cggaagctgt gccggggcag aagaccatca tggaggaacc
     8641 gacggagacg gcaccggaca acggtcagac gataaaagaa aatcccggtg cagaaaaaac
     8701 acaggaggca ccacagctgc agaatctgca gaaactgtta aagcaggggc tggagacgaa
     8761 agatattccc ttgctgcgca gtattctcca taattccaaa gtggcagagc ttccggcaaa
     8821 acttctggcg gatcgttgga gcataaaacc agaggatgtg gaatctccgg agaaggtgga
     8881 ggaactttac cagaagctgg gaaaacagtt gaaggggttg agtaatcttt tggaagaaaa
     8941 cggacagcgt ggttcaagtg cttatcagaa cgtgacgaat ttaagtcaga acgtggattt
     9001 cctgcaacag atcaaccaga cctatgccta tatccagctg ccgctgcatc tacggcaggg
     9061 agagcataag acgggagaac tgttcgtata taccaacaag aaaaatcttg ccagaaaaga
     9121 cggacaggtc agcgcactgc tgcatctgga tatggaacat ctggggccac tggatgtata
     9181 tgtggctctg aaggatacca aagtcagcac caaattttat gtacagaatg atgcaatcct
     9241 ggattacctg gaggctaata tggatgtgct gacagagcgg ctgcaaaaaa gaggctatga
     9301 ttgtaaatgt gagacaacac tgcgtaccga attacagcag acggcgcagg ccatggcacc
     9361 gcttcttaag acagaaggaa gtgttccggt agcacaatat gcctttgatg tgagaacata
     9421 gctcatccat aggaaacaga cgtgactgct agcaggcagc ggatgtttcc tatgagagga
     9481 gcggacaaac atgagtatgc aggacgatag tccggaatac gagtgattgt tacgatttcg
     9541 agagtgcgta gcacggagaa aacgtatatg ttagaagagc atagaggaca acagaatgaa
     9601 tgacgagaaa aagaaagtaa aacaggccgt agcactggaa tacgatccct cggatgaggc
     9661 acccagagtc atagccagcg gcaagggaat tctggcggag aagatcatag agaaggccca
     9721 ggagagcagt gtgccgatcc atcgggatga caagctggcg gatacactgt cccggctgga
     9781 catcggcgag atgatcccgc cggagttgta tgaagtggtg gcggagattc tggtatttgt
     9841 ggatgctgtg gatcaccgca tggtaaagga aagtgtggca aacgggggaa aacataaggg
     9901 ttgaatacca gacagacggg gaatgtatat gagcagatgg ctgcggacta tctggagaaa
     9961 cagggaatgc gtatcctgga acggaatttc cgcagaggaa ggaacggtga gatcgacatt
    10021 atcggcagag acgggaaata tcttgtgttt gtggaagtga aataccgttc cggtgatgaa
    10081 aagggaaatg cggcggaggc cgtgacaacg gcgaaacaga gaaccatctg cagagtggcg
    10141 gactattacc gttatctgca tcattatggg gaggatacct gggtgcgcta tgatgtggtg
    10201 gcaatccagg gggaggcact tcactggatt ccaaatgctt ttccacacca ttatagtgca
    10261 aggagataga ataatgtatc cattgatcag aacagactgc agagacacag acagtataca
    10321 gcagagacaa aaaggggaac tggagtatct ggtattcccg aagctggaag agaccggtgt
    10381 ggtggaacat ctgtttacca caaggaccgg tggtgtcagc aacggaatct attccactat
    10441 gaacctgagt ttttccagag gagatgacct ggaatgtgtc agagagaatt acagacggat
    10501 tggcgaagta ctgggaacag acccggaaca tatggtggca tcgaaacaga cccataccac
    10561 gaatattcat ctggtgacga aagcagatgc cggaaatggg atcaccagac catcggttta
    10621 tgatgatata gacggtctgg ctacggatat ccccggaatc gcactggtga ctttttatgc
    10681 agactgtgtt cctctgtatt tcgtggatcc ggtacacagg gccatcggac tggcgcattc
    10741 cggctggaga ggaacggtag cgggaatggg agcttgtatg gtacgatata tgcaggaaca
    10801 ttttaacagc cgcccggagg agctggtggc ggccatcgga ccgtccatct gtgtggattg
    10861 ttatgaagtg agtgaagaag tggcggaaca gttccgggaa ggctttccgg aggatgtgtt
    10921 gcagccgggc aaagcaccgg gtaaatatca gcttgacctg tggaaagcca atcagagcat
    10981 tctgttaagg gcggggatcc agccggaaca tatggcggta acgaacgtct gtacctgcca
    11041 caatccggag tatctgtttt cccaccgggc aagtcacgga cagaggggaa atctggcagc
    11101 atttttaatg ctgaaagagt cttgggttcc tgagtgcctg acgggaatta agaaaattta
    11161 agaaaaaaca cgaaaatatt taaaaatatt atgtttaaat agaaataacg aaaatgatgg
    11221 ggaaccgggc agtaacggag aaatgaggga agtatggcaa atattcttgt atgtgatgat
    11281 gacagagaga tcgtggatgc catcgagatc tatctgagtc aggatggcta taaaatatat
    11341 aaagcgtatg atggggaaca ggcaattcag atcctggaca aggaggatat ccatctgttg
    11401 atcatggata tcatgatgcc ccggctggat ggcatacggg ctacattgaa gatccgggaa
    11461 tacagcagca ttcccattat tatcctgtcg gccaaatcgg aagacacgga caagatcctg
    11521 gggctgaata tcggtgcgga tgattatatc tgcaagccct ttaatccgtt ggaactagtg
    11581 gcgagagtca aatccaactt gcgcagatat acctccctgg ggagcctgac cggtgagaac
    11641 aaagcaatct atcaggtggg cggactgatc ctcaatgatg ataccaaaca ggtgacagtg
    11701 gacgatgaac cggtaaaaat gacacctatc gaatataata ttttattact tttgatgaag
    11761 aaccagggca gggtattttc catcaatcag atctatgaaa gcatctggaa tgaggatgcc
    11821 atcggagcgg acaataccgt ggcggtacac atccggcata tccgtgaaaa aatcgagatc
    11881 aatcccaagg aaccccggta tctgaaggtg gtatggggag taggctataa gatcgacaag
    11941 gagcaggcat gagacgacct acattgaaac ggctgatgct gggactggtg cagcagacgg
    12001 cggctttgct tctgctggtg gcaatcgcgg cggttttgtt caattcctat ctggcggtag
    12061 acacagcgga tggcacaaaa gtatatgaat tatcccctct ggatgcggaa acggaatttg
    12121 aagattccgt gattttccat gatctgttcc agagctcggt atcggatatt atccagctta
    12181 tggtgatcaa aggacagatg gagaccaatg gaagctttga tccgcataag catgttgata
    12241 tcacggaatt tgcttcaggg aaaaccggtg gtgcagattg ccctgtaaca gcagtttatg
    12301 agctggaaga tctgatcaaa tggggaaaat acggtgcgga atatacagat cgtattatga
    12361 gtatgtcgga cttcgtgaat tatttcggac cgatagacct ggagtcgaac ttccgcgtgg
    12421 atgcggacgg acagctgatg ttcgtggcag gcggagagca gacggaggag cagaaggaag
    12481 cagtcgcaca ggcaatcgag gcaattccgg agagccagag aaccgatcgg ctggaagacc
    12541 tggctttcac atacattgtg aaagagagtg tacaggatat ccgggtatcc agagaagacg
    12601 atggtacgct gacggtatat ttcccgatgc tggtgaaccg ttatgccacc gtagacggag
    12661 agaagcagct gacagcctgt gcggacaact ggatcgaata tattgcgctg cagaacaatc
    12721 tggagatgac gatccagacg ttgtcagcca actatgagca gtatcagaac tgtaatgatc
    12781 tgtataaaga agatgcgggc aatctgaaat atgcggtccg tatgatgaca acagatggag
    12841 ttagcagaac ctatacgaat gtatcggagc ttgagaacag ttccgataat gatattacgg
    12901 attatttttc ggagtacaga cggtatctga tctattatcc ggacagtctg gaatttaccg
    12961 gcaacacagg aatgactgag ggccagatct atcagtatct gaaagagtat ggttatgtgc
    13021 atccggatat gactcatatc tggatcgcag tggataccaa ttatccggtg gagggagatt
    13081 ccttttacaa tgccaatgtg gtgttccaga gaatcgttcc gaatatctgg tatctgatcg
    13141 gcggagggat cctgctggca gtattgtggc tgctggtggg tatttatctt accgtaacgg
    13201 ccggcgtggc ttacgatgag gaggatgagc cggttctgta cctgaacggt atcgatcatg
    13261 tctggatcga gtttatggcg cttttgctgg tgggatttat ctatggcgga agaatcggat
    13321 acgattatct gatgaatgtg gccaataagg tatatctcag ccattccgag atccaggggc
    13381 gggagatcac acggctgaca gcctacggtg tctttgcggt gtatggcttc tgcgtaagca
    13441 tgggaatcaa tgttttctgg tacagcctga tccgcagaat caaatcccac aatatgtgga
    13501 gtgacagttt tctgcactgg atcgtcacaa gcttcagtaa ggcggtacat tttgtggttt
    13561 cccacagaaa ttccgccatc agctcactga tcccgtataa tctgtttcta ctggtgaatc
    13621 tggcaggaat tttcggagct tatctgctgc ggggaaaaag cgctctgtgg gtgcttccgg
    13681 cactgggggc tattgtgttg gacggcattg tgggggtact aaggttcaag caaaaagcgg
    13741 agcagatcga tatcgtggaa ggaatccgca ggatccgtga tggagaagtg gattataagc
    13801 tggatgtgga atctcttcat ggggacaacc gtgagatggc ggatgcagtc aataacatcg
    13861 gagagggtat ccgcaaggcg gtaagcacca gcatgaagga tgagcagatg aaatcggatc
    13921 tgatcaccaa tgtctcccat gatattaaga caccgctgac ttccattatc aattatgtgg
    13981 atctgctgaa acgcctaaag atcacggaag aaccggccag aagctatatt gaggtgctgg
    14041 acagcaaatc ccagagactg aaacagttga cggatgatct ggtggaggct tcgaaaatct
    14101 cctccggtaa cattgtgctg aatctggaac agttgaacct gacggaactg ctgaatcagg
    14161 cagtgggaga attctccgac agaatggaag agaaaaagct gcaggtgatc tttgacggaa
    14221 atgatgtggc cggcatgatc tatgccgaca gccgcaggat gtggagaatc attgaaaatc
    14281 tgtttcagaa tatctgcaag tatgccctgg agggaaccag agtctatctg gagatgcagg
    14341 taaaggacgg aagagtcatc gcttctctga aaaatatttc cgaccggcct atgaatctca
    14401 agggtgagga actgtcagag agatttatcc ggggagatgc ttcccgtact accgagggaa
    14461 gtggtctggg tctgtatatt gcgaaaaatc tgaccaaggc acagcacgga gaattccaga
    14521 ttcagctgga tggagatctg tttaagatta tattggattt cccggaatac cataagccgg
    14581 aagaacccgc cactgagcag ctgaaggaga ccgtgtcgaa agaggtttag aaatggcgaa
    14641 ataagaaata ctgaaataag caatataact gaaaaacgca gaaaccataa cggcttctgc
    14701 gtttttttta tgcgttgtca gatctgttgg aaattaaaaa tgaaattcgc tcatttccgt
    14761 acgattgttg taggcttcta caataccgtg gatcttttcg gtggtagcca tgacattgga
    14821 cagagaagcg tcatggttca taaagtcgat cagggaagca atgaacttgc tcatatcggc
    14881 ttccacgaaa taaggtctgg tgtagatctc aggcggtaga taggtaagat tcgtagtaat
    14941 gaccttatcg aaatcaccat gctcgtaagc agcgtcaaaa ttcttcagac cgtcggtgaa
    15001 caagccgaaa gtacagcaga tatatacgcg tctggctttc atggccttta actgttttgc
    15061 agtatccagc atacttccac cggaggagat catgtcatcg atgatgatga tatccttacc
    15121 ttcaatattg tcacctaaga attcgtgagc aacgataggg tttttgccgt tgacgatggt
    15181 ggaatagtca cgtctcttgt agaacatacc agtatcgaca cccagaacgc tggcaaaata
    15241 aatggcacga tccagagctc cttcatccgg gctgatgact aacaaatgat ccttgtccac
    15301 gatcatatcg tcttcggaat ttaacaagga cttgatgaac tggtaagggg tggtaaaatt
    15361 atcaaatccg ttcagcggag tggagttctg tactctggga tcatgggcat caaaagtgat
    15421 gaagttatca attcccatat cccgtaactc ttccagtgca taagcacagt ccagggattc
    15481 tctgccgctg cggcgatgct gtctgccttc atagaggaaa ggcatgatga cgttgatacg
    15541 tctggccttg ccgttgcagg cggcgatgac acgcttcaga tcctggtagt ggtcatcagg
    15601 agacatgtgg ttggtatagc cgttcatgtt gtaagtaata ctatgattgc atacatcggt
    15661 gagaatgaac agatccttac cgcggatggt ctcgctgatc tgcgctttgg cttcaccgct
    15721 tccgaaacgg ggcacatcca cgtctacgag atagttgctc tcgatatagc cgtgaaaggc
    15781 aggatcgttt ttcactttgt cattatggat actcttacgg aattctacca tgtagtcatt
    15841 gatgcggcgt cccatttttt ctgcggaagg cagagcgatg atcttcaaag gagccacggg
    15901 catggcattt tccagttcgt gtaaattagg catgatatcc ctcctggacg agctttattg
    15961 ttcgtaaaaa tatgcagatc ttccttcgca aaagcgcatc ttcgatgctt aattttgcgg
    16021 aactattctg aatataacaa ttaatttata acattatgct agtgacacgt caaggaattt
    16081 cttgtacaat ggataggaga aagatggtta ggtgaattag aatgaagaaa aaagaacata
    16141 ttgtaaaaac agtaaagccg ggaagcatcg cggaggaact ggagatcgct cccggagata
    16201 aagtactggc catagataat acagagatcg aagacatttt tgattatcag tttctgacac
    16261 aggatactta tattgagatg ctggtagaga agccggacgg agagcagtgg ctcttagaga
    16321 tcgacaagga atttgatgag gatatgggca tcgaatttga gaacggtctg atggacgaat
    16381 accgtcattg ccataacaaa tgcattttct gctttatcga tcagatgccg aagggcatgc
    16441 gggatacctt gtattttaag gatgacgatt cccgtctgtc ttttttgcaa ggaaattatg
    16501 tgacactgac aaatatgtca gaccatgata tcgacagaat ctgcagatat catctgtcgc
    16561 ccatcaatat ttccttccag accatgaacc cggatctgcg ttgtaagatg ctgaataacc
    16621 gtttcgccgg ggaggcactg aaaaaggtgg atatcctgaa tgaagccgga atccggatga
    16681 acggacagat cgtactctgc aagggcgtaa acgatggcaa ggaactggaa tacagcatcc
    16741 ggaagctgat ggaatatctg ccaaatgtgg aaagtgtctc agtggtgccg gtaggattat
    16801 ccaggtaccg ggagggatta tatccgttag agccgtttaa tgcgcaggat gccggtgaga
    16861 tcattgatct gatcgagaaa taccagaaga tctgcatgga aaaatatggg acacacttta
    16921 tccaggctag tgatgaatgg tacattctgg cgggcagaga ggtgccggaa gaggaacgtt
    16981 acgacggata tctgcagttg gagaacggtg ttggaatgat ccgcctgcta ttggatgaat
    17041 tccacgacgg actgaagagg cgtaccgcag aaaaggaagc gggaaaaaag ccatcctggg
    17101 aaggcaccag agagatctct ctggctacgg gaagactggc gtttccttat ttaaagaaga
    17161 tgtcggaaga aatgatgtcg gaataccccg gcctgcagat acatgtctat gaggtcatca
    17221 atgaattttt cggagagctg atcacggtgt ccggacttct gacgggacag gatatcgtaa
    17281 agcagttaaa ggacaaaaat ctgggagaag cattgtatct tccccggaat gtactcagaa
    17341 gcggagcgga tctgttcctg gatgattata cgctggcaga cgtggagggg gctttacaag
    17401 tgcccgtgaa tattgtaaaa tcaagcggat atgatcttgt ggatgccctg cttggagaag
    17461 atgcatctga atagatttta agatggtaaa taagaaacgg aggaaattat ggcaaagaga
    17521 aaaaccaagc ccatcgttgc ggtggtggga cgcccgaatg tcggtaagtc caccctgttc
    17581 aatgcactgg ctggcgaaaa tatatctatt gtgaaagata cacccggtat taccagagac
    17641 cgtatctatg cggatatcca ctggctggat atgacattta ccctgatcga caccggaggt
    17701 atcgagccgg acagcaagga cgtgatcctg tcccagatgc gggaacaggc agagatcgct
    17761 atggagactg cagacgtgat cattttcctg gtggatgtaa agcagggact ggtggatgcg
    17821 gattccaagg tggcagacat gctgcgccgt tcccacaagc ccgtggtact ggtggtcaat
    17881 aaggtggaca gcttccagaa gtatatggcg gatgtctacg aattctacaa tctgggtatt
    17941 ggcgatcccc atccgatttc tgcggtgaat cgtctgggaa tcggtgacat gctggaggct
    18001 gtaacggaat attttgataa ggaacaggca gaggaagaag aggatgaccg cacccgtgtg
    18061 gccatcgtcg gaaagcccaa tgtcggcaag tcttccctga tcaacaaact gctgggtgag
    18121 aaccgtctga tcgtttctga tatcgcgggt accaccagag atgcggtgga taccgagatc
    18181 acttataacg gcaaagaata tgtcttcatt gatactgccg gtcttcgccg gaagaacaag
    18241 atcaaggaag aactggagcg ttatatgatc gtccgtaccg tcagtgccgt agagcgtgcc
    18301 gaggtggtgg tactgatgat cgatgcagag gaaggtgtta cggagcagga tgcgaagatc
    18361 gccggaatcg cccatgagag aggaaaggct acgatcatcg tggtgaacaa atgggatgct
    18421 atcgaaaagg atgacaagac catttataaa tttaccgaaa aggtgcgtaa taccttatcc
    18481 ttcatgccct atgcagagct tctgttcgtc tctgcgaaaa caggacagag actgcccaag
    18541 ctgttcgaga ctatcgatat cgtcagtgag aatcacgcca tgcgtgtggc taccggtgtg
    18601 ctgaatgaga tcatggcaga ggccgtggcc atgcagcagc caccttccga caagggtaag
    18661 agactgagat tgtattacat tacccaggta gcggtaaagc cccctacctt tgtaatcttt
    18721 gtcaatgata aggagctgat gcatttctcc tataccagat atattgagaa tcagatccgg
    18781 gagacctttg gcttcaaggg aactcccctc cggtttatta tcagagaacg aaaggaaaaa
    18841 gatcaataat ggaacgtgtt atctgtttac tcatcggtta tgtgttcggc ctgtttcaga
    18901 ccgcatactt ttacgggaag gcaaaaggaa tcgatatccg taagtacgga agcggcaatg
    18961 ccggtaccac caatgccctg agagtgctgg gaaccaaggc aggactgatc gtactggcgg
    19021 gagactgctt aaagtgtat
//
LOCUS       DAWEJW010000056        18547 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1140_length_18547_cov_54.213876, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000056 DAWEJW010000000
VERSION     DAWEJW010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 18547)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18547)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18547
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1140_length_18547_cov_54.213876"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5502 a         3920 c         4734 g         4391 t
ORIGIN      
        1 agagcgcaag cgtgccgcgt ttcttatacc ctgccaaggg ctacggggat atttcgctaa
       61 ctttcaattt acttgtcttg tgcaactgtt catatcgcaa agttctgaca acttacaaaa
      121 atatgaaata attaacaata tttacttgta atagtaacat atatttagta aaatacacgt
      181 aaggggttaa aaatccaatc caacggctgg acaaaatacc caaaatgcag aataaatgga
      241 ggatgggata tgaatattta cacaacagac aagatccgta acgtagtatt actgggccat
      301 ggcggcagtg gcaagaccag tcttgcagaa gcctttggtt atctgtccgg tatcacctcc
      361 cgtatgggca aggtagatga tggcaatacc ctcagtgatt acagtaaaga agaacagaaa
      421 cgtcatttct ctatcagtac ctccgtgatc cccattgagt gggaaggata caagatcaat
      481 attattgata caccgggtta ttttgatttc gtgggagaag tggaagaggc agtcagtgcc
      541 gcaggagcag ccatcatagt agttaacggc aagtccggca tcgaagtcgg cacccagaag
      601 gcatgggagc tttgcgagaa atacaagctg ccccgtttta tctatgtctc caacatggac
      661 gtggataatg cctccttccg tcaggtagtg gaagatatga ctaatctgta cggcaagaag
      721 atggcaccgt ttcatctgcc aatccgtgag aacgagaaat tcgtaggata catcaatgtt
      781 atcactgaga gcggtaaccg ctgggagggt aaggaagttg ttccctgtga ggtaccggat
      841 tacagccagg ccaacctgca gatctgccgg gatactctga tggaagccgt agccgagacc
      901 agtgaagaat tcatggagcg gtatttcggc ggagagacct tctctgaggc agagatccgt
      961 gcagcactcc gtaccaatgt ctgcgatggc agcatcgttc ccatgaccat gggttccaac
     1021 atcctgtgtc agggtatgta cacactgtta gatgatattg tcaaatacat gcccagcccc
     1081 gagaacagag aagtggcggg catcaattta aagaccaatg agatttacca tgcggattac
     1141 gatttcgcca aggcaaaatc cgcctatatc tggaagacca tagtggatcc tttcatcgga
     1201 aaatactctc tgatcaaggt aaactccggt gtacttaaga ccgacgacct gctgtacaac
     1261 gtagaccgtg acattgagga aaagatcggc aaaatctatg tactgcaggg caataagccc
     1321 attgaagtca gcgaactgca tgccggagat atcggagcac tggcgaaact gactgcagcg
     1381 agaaccggca acagtctctc taccaaggcc acgaccatca aatatggcaa gttcgatatt
     1441 tccactcctt atacctatat gcggtataag ccgaagaata aggcggacgt ggataagatc
     1501 tcccaggcat tgcagaagat gacccatgag gatctgacca tcaaagtagt aaatgatgca
     1561 gaaaaccgtc agaccctgct ctatggtatg ggcgaccagc atctggatat cgtggtaagc
     1621 agactgctga acgaatacaa agtagagatt gagctgtcca gaccgaagat cgcatacaaa
     1681 gagactatca agaaacagtc cgatgtggaa tataagtaca agaaacagtc cggaggacac
     1741 ggtcagtacg gtcatgtaaa aatgcgtttt tcaccttccg gcgatctgac caaaccctat
     1801 gagttcgcaa cagaagtcgt aggtggcgcg gtaccgaaga atttcttccc tgcggtggaa
     1861 aaaggtattc aggaatccgt aggtagagga cctctggcgg cctacccggt agtcggtgtc
     1921 aaagcggtac tgtatgatgg atcctatcat ccggtagatt cttcggaaat ggcatttaag
     1981 accgcggcta tccaggcttt caaaaaaggt gtcatggaag cagaccccgt tctgttggag
     2041 cccatcatgt ccttaaaggt taccgtgccg gatgcctata ccggagacat catgggagat
     2101 ctgaacaaga gaagaggccg ggtgctgggc atgacaccca tgagcggcgg caagcagatc
     2161 atcgaagcgg atattcccat gagcggatta ttcggttatt gcacagatct gcgttccatg
     2221 accggaggca gaggggatta ttcctatgaa tttgcccgct acgagcaggc accttccgac
     2281 gtacaggaga aggaagtagc ggccagagca gcaaaggttg cggagaacaa tgcagaagac
     2341 taacgagtaa ttacataaca tacacaacaa aaagagaccg gttggctttc cggtctcttt
     2401 ttcggtattg tgcagtaaat atcatcgata tcgctgcatc agattgatca gtaatgtgat
     2461 ccatgcaaac atgattcatc acctctcgta tacagcatat gccccggcag acgaaaatat
     2521 gcaaaaaacc atgcggatag agccgcagtc cttgacagta tcgggaaaac acgataaaat
     2581 aatcgatagt tttttaacga cttgagaaca aaaaatttaa atagaaagct tctcatagtt
     2641 taggaggaaa taattatggc agtaccttac aatcacagag aggtagagaa aaaatggcag
     2701 actgtctggg atgatgaaaa agcattcaag acttccgacg atttctccaa gcccaaatat
     2761 tacgcactgg tagagttccc ttatccttca ggacagggat tacatgtagg tcatccgaga
     2821 ccttatactg cactggatat cgtagcacgt aagcgcagaa tgcagggcta taatgtgctg
     2881 tatcccatgg gatgggatgc attcggtctc cctaccgaga actacgcgat caagaatcat
     2941 atccatccca agatcgtaac caagaacaac gtggcaagat tcaagaatca gctgcattcc
     3001 ttaggttatt ctttcgactg ggacagagag atcaacacca cagatcccga gtattatcac
     3061 tggacccagt ggatcttctt aaagctgttc aaggcaggac tggcttacaa aacggagatg
     3121 cctatcaact ggtgtacctc ctgtaaggta ggtctggcaa atgaggaagt agtgaacggc
     3181 gtctgcgaac gttgcggttc cgaagtcatc cgtaaggtca agagccagtg gatgttaaag
     3241 atcaccgaat atgctgacaa gctgatccag ggactggata ccgtagatta tatcgagcgt
     3301 gtcaaagtct cccagaagaa ctggatcggt aagtcccagg gtgccgaggt tgatttcacc
     3361 ctgacaggca aggatgagaa gctgcgcatt tacaccacca gaccggatac gctgttcggt
     3421 gttacctaca tggtagtatc tccggagcat cctgttctgg ataaatataa agatgagatc
     3481 aagaactggg atgacatcgt ggcatatcgc gagatggctg cgaagaagtc cgattttgaa
     3541 cgtaccgaac tggcaaagga taagaccggt gtctgcattc agggtctgac cgctaccaac
     3601 cccgtgaatg gcaaggaaat ccctgtttgg gtatccgatt atgtactgat gacttacggt
     3661 accggcgcta tcatggcagt tcctgcccac gatgagagag actgggagtt cgcgaagaaa
     3721 ttcggtatgc ctatcattga agttgttgca gggagccctg tcagcgtaga agaagctgta
     3781 tataccgatg ttgctgatgg tacattggtc aattccgatt tcctgaacgg cctgtctgtg
     3841 gcagaagcca aagagaagat catgaactat ctggaagaga agggcatcgg taaccgcaag
     3901 accaattata aattaagaga ctgggtattc tccagacagc gttactgggg tgagccgatc
     3961 cctatcgtac attgcgataa gtgcggatat gttcccattg atgagagcga gcttcctctg
     4021 cagttacccg atgtagagag ctacatgcct accgataccg gagaatctcc tttggctgcc
     4081 atgactgact gggtaaatac cacctgtccc tgctgtggcg gtccggcaaa gagagagacc
     4141 gataccatgc ctcagtgggc aggttcttcc tggtattacc tgcgttatac cgatcctcac
     4201 aatgacaagg cactggcaag tcctgaagca ttgaagtact ggctgccggt tgactggtac
     4261 aacggcggta tggaacatac cacactgcat cttttgtact cccgtttctg gcacaagttc
     4321 ctgtatgatc agggggtagt acctactcca gaaccttacc agaagagaac cagccacggt
     4381 atgatccttg gctccaacgg cgagaagatg tccaagtccc gtggcaatgt ggtaaatccg
     4441 gacgatatcg tacaggatta cggtgcagat actctgcgta cctatgagat gttcatcggt
     4501 gcctttgatc tgagtgcttc ctggagtgag gaaggcgtaa agggctgccg cagattcctg
     4561 gagagagtat ggaagttaca ggatatattg acatccgagg aaggctacag cgcagatctg
     4621 gagaccaaga tgcaccagac catcaagaag gtcagcagcg atttcgagaa cctgaagtac
     4681 aacaccgcca tcgcggctat gatgtccctg atcaatgagt tctacaagaa gaactctatt
     4741 accagaggcg aatacaagac actgcttact ctgctgaacc ccgtggctcc tcatattact
     4801 gaggaattgt ggcagactgc cggattcgag ggcagactgt atcaggcagc atggcctgaa
     4861 ttcgaggaag agaagaccgt agagagcgtt gttgagatcg gtgttcaagt caacggcaag
     4921 atgcgtgtga ctatcaatgt ttccaaggat gagaccaagg aaaatgtcat cgccatggca
     4981 aaagaagctc tgggtgacaa gctgaccggc aacattgtaa aagaaatcta tgttcccggt
     5041 agaatcgtca acatcgtggc aaaatagttc agccatggac aaataagtga aataaaatca
     5101 tgcttgcatg attttatgat cgaatatgtc catgaggtaa acgccgtctt atgaacaaag
     5161 gtagtcacgt agtgatggaa agcgtgataa cgcgactttg cgaatggcgt tggctgaacg
     5221 ttgaataaaa taactcttga cagacattaa gcctgccggg agttattttt ttgtgatcgg
     5281 cttttctcca gaatattttc aatctgacga agctccgatt ccgtactgta ttttttgata
     5341 gcggtgatgg cggcctgcat ttcctgcggg gtaaaagtca ggtgttcctg tttcgcttta
     5401 cgcatggcgg gcatgagaag tgccatcatt tctttttgac ttttaccgtg cccctgggca
     5461 aaaagtttcg agagaaaatc cagtttttct ctggggatgt ccttcacgag atcatcctgc
     5521 atccagaggg gtgtcttgga atagtccatt tttctgacct cctgtgtctg attttgcagt
     5581 ggaaaaaaga ccggacaaca tagacggatc catgccggag agatcggaga agccctccgg
     5641 aaacaattcc tgcatggtct gcaccatttc catcatttcc tggaaattat gaatattgtc
     5701 catcatttgt gtgatttgtc gaagagctga tttctgagtc tcgtcgcaca agggcatgag
     5761 atcattacac agttcagtgg cagtttcttc gtttgcagaa gagggagcaa tgcacagaga
     5821 agtctccgga tgcctgcgaa aaagtgttag cgtgtattgc aactccgaca tttttatgta
     5881 aacagcaagt cctttcagca gccggggatc catataataa gccagtattt tcagaatctg
     5941 aatgcggttg gaagtgaata atgtgtcaaa ggcagccgcc ttgtcgattc tcttatcatc
     6001 catgtaccga taccttcctg caaaagagtt actagtattt tatgagagcg gcagcatgct
     6061 tatgccgatc aatccttcgg ggtacagaaa agtccgtacc ccggagtgat cgtgcctaca
     6121 aagctccggg agatcccgga aagcagtatc agagattagc aaccgcaacc gttgttgtta
     6181 ccgcagccac cgccgcagaa gcaggacaga agcaggatcc agatcagcca ttcacagcag
     6241 tcattgccgc cgcagccatt gttgttgccc agcagcccgc agccgttgtt gttaccgcag
     6301 ccaccgccgc agaacagcaa caggatgatc cagatgcagc tgttgccgaa gataccgtta
     6361 ccacagttgt tattgccgca ggaattattg cagccgcaat ttgtagcagt taagtcactc
     6421 atttttagtg cctccacatt ttatttatgc tctattgtat gcgggggagc ctgtcaatgt
     6481 ttccggaaaa gacttgcaat aatttccgga tatagtaaaa tatagatatc tatatagaca
     6541 tgcatagatt gtgaaaagac agattttaaa ttaaggatag atagatggca cagaagatat
     6601 atgtggtaga ggggcgaaag ttccgcacgg aggcggatta tcagaatgcc ctgaaggatc
     6661 agcaggtgat cgagaatctt cgggtacaga cgaagaagat gagcctggca gatcagaaga
     6721 aactgctgga agtgatccgt agcggcaggt gtagattcca tagtattctg ggacaggatt
     6781 acatggaaga gctgtcggag gacattcaaa gacaggaagg aaaagtaccg gaaacatctc
     6841 cggcgaaagg acattttctt cagaaagaaa atacaaaaac aatcgggaag gcccaaactc
     6901 ccaaaaataa aaaagaagca tcaaagaaaa aagaaactgt aaaaaaagaa agtcctgata
     6961 aaaaagttcc caaaaagagg ccgacaacaa aaaagaagac ttctgtgaag aagaaagcag
     7021 aaccgcagtt tgacgaggca gagattgaca aaatcgttca ggaagaattg aaaaaacagg
     7081 agaaacgaag gaaacttctt gtggtctgtt gcagtgtgat cgcagtggca tgccttggct
     7141 atttcggaat ttataactgg tataacatcc gtactgccga taattacaag caattaagtg
     7201 aattgaagga taaagagccg gcaaccggtc agaatcagga tcctgtgatc cattacaccg
     7261 cggatgaaac gcagagtacg ccccctccgg tactggatga atataaaaat ttattaaata
     7321 agaacaaaag actaatcgga tgggtaaaaa ttgacgatac taatatagat taccctgtaa
     7381 tgcagacaac ggataatgaa tattatcttg atcacaatct gaatcaggag tatgataaaa
     7441 acggatccat tttcatggac aaggattgtg atgtgctcaa gcccagcaca aacttcattt
     7501 tgtatggaca tcatatgaag agcgggcaga tgttcggaag cttatcccta tacagcgacc
     7561 agagctatta tgagaaacat ccttgcatac agtttgatac catttatgaa aaaggccagt
     7621 atgagatcat gtacgtattc agatcccggg tctatagtga agatgaaatc gtatttaagt
     7681 actatcagtt tatagacgcc cagagcgagc aggaatttga ctcttatatg aatgatatgg
     7741 cagaaatgtc attatatgac acgggagtca cagcttcgtt cggtgaccag cttttaacgt
     7801 tgtctacctg tgattatcag gagaaaaacg gcagatttgt agtggttgcc aaaaaggtga
     7861 cgacaaagga gtaaaggatg actaagggta aaaagaagac taacagaaga ttaaaaaaga
     7921 cagtaaggaa gaccctgggg acattgtttc tgatctcggc aattatagta gcggcaatcc
     7981 ctacggaagg actgcgtgca gcagatggaa cagaaaagga ggttacggaa tcttatctgc
     8041 aacagatgaa gattccttat ctggacagta aggaagaaat ctataccacc ggtgacggta
     8101 agtatcagtt tgcctatgta acggacggcg gcagtgataa gggtgccgtg atcctgggat
     8161 acaacggtgg ctatctggaa ggtaacaacc tggtaattcc cgatggactg gatgcgtata
     8221 agaaatattc ttacagtgat ggaaccaacg gcggttatgt cgctgtaggt aaggaaaatg
     8281 atttcctgtt ctatgcagtg aatcagacga caactgatcc ggtaaccggg gcagagattg
     8341 tgatcagcac atcgtatcag ccctgccgtt atgaagaccg tgcccagtgg gagaatttat
     8401 catctgaaga actgtattat cacaagtcgg atactgtatc cggtggtgat cttgtacctg
     8461 tttatgagaa aacgacagat tccaataaac agcgtatcaa aggtgcagag gtatggtata
     8521 tcggcaacca gtatctgcag gaaggaaccg gaaaagatgc aggtacctgg aaaattgcag
     8581 gaccgatcac cagtagtgat aaggggattt ttgccggaaa tggtaatatt cacacactga
     8641 ccatcggaaa gagcgtaaaa ggagtgggtg tatatgcatt ttatggctgt accggtatga
     8701 gcagcatttc tttgggaaat ggtctgaata ttattggtga ttatgcgttt gccaactgcc
     8761 ttactatgac cagtgtaaat ctggatagga tcagcaatct gacaacgatt tcggattacg
     8821 catttgccaa ctgccagtcc atggaaaaat ttacggttcc tattaatgta acaacaattg
     8881 gcaataatgt atttgaagac tgttattcca tgataggcat taatatgtcc aacgcagaag
     8941 aaaataacgg tttctctgtg gaagaggaaa tggcttccac aaataatgca acagcattaa
     9001 acaattttgg gaaaaatgta tttaaaggat gtgcaaattt aagatctgtg acattcccca
     9061 gaactttcag caatgccaac ggcccggtat ccggatctat tgatgtatcc gagttccggg
     9121 gatgcgagaa tctgaaatat gtaactgcag caaacccgtc tttgaagatc acggggggag
     9181 cagtagatac ttcatatacc tttgattcct tcaaagcgga tgtaccggca gagttttatc
     9241 tggaaagtgt tgacagctcc cagttccata cctgtgcgac gaataattat atggcattca
     9301 gttatctgaa ttccgacgga accaagcaga atctgtatga actgacagtt caggaaacaa
     9361 gtggcaaaaa ggctgtttac agagtgaatt cctctaatca gctggtatac tgtgaatttg
     9421 acagcggtct ggaaactgtt accatgccgg atactgtcgg accttaccag attacagtga
     9481 tcggctccgg cattttccgt aataactgtt atctgaggag aattacgatt ccctcctcta
     9541 ttactacgat tgaagacagt gcgtttaaag gctgccataa tttgaaagat gtcattttca
     9601 aggagcctgt caatattacc agtatcggta cagatgcatt caagacccag gaggtggaat
     9661 atcataaggc agattgcggt aacaaggacg caactgatgt acagcaattg tattttacag
     9721 gccctatctc ggatgattcg gcaccgttca attatgcaat ggatccgaac agctatatta
     9781 acagaggaaa ccaggaaaga acctatatta cctattacag tggctggccg aagaatctgg
     9841 aagtaaagta taacccggat acggacaaaa atgaattggc agattatcct acgtttaaag
     9901 aaattcagtc cggttcgaaa tataccacag gcaattatgc ttatatgaca ctggaatacc
     9961 aggaggcggc taagactgcg gtagataaat atcttggtac gaattcagaa cctatgacgg
    10021 attatgaacg tgagatcatc aatgcggcac tgcagatcga attaccttac ggtattgaaa
    10081 ccattaaaga aaatctgttt actgccacag aaccttcgga agctctgccg tccaatacga
    10141 ccaagacatt gaccgcttac ggactgttgg agattggcaa ggagactttc aaggattgta
    10201 cgaatctgag tggtgtctat ctgaaagaca gaactacgaa gattgatgat tatgctttta
    10261 agggatgtac aaatctgacc ggtgtggcag tacctgccac cacaacaaca atgggaaaac
    10321 gtcctttcac cggctgtgag aaactgacaa cggtggattt ccagggaagt ccttatttca
    10381 cctgtgataa ttctattatc tacagtctgg aaaacggaaa gaagaaagat atcattgaat
    10441 ttctggaagg aagagccagc ggagtggtag ctcctacgga agtagaggga gtcaccggaa
    10501 ttgctgagga agcatttgcg ggcaccaagg taagttccgt ggatctgcgt acctcctttg
    10561 tgacagatat tccggaaggt gcttttatgg atactccgaa gctgttcgca gtttatctgc
    10621 cggatacctg taagtccatt agtaaggatt cctttacgaa cagtagtatt gcctacatgg
    10681 aagtgccgga aagtgtcagc tatctggata atgatgcctt cggtggaacc accgataaga
    10741 gtgcactgca gttctactgc gtagatggca gtaatgcata tatctatgca acaaagaacg
    10801 gtattatgac gacctcaaag ccgctggaac tgttctatac cgtaactttc tgggattggg
    10861 attctacgct tctggataca cagacggtag cagcaggggg agatgcagtt ccccctgaag
    10921 ttaaaggcag agagggatat acccttaccg gatgggttcc ggattatcat ggggtacagg
    10981 ccgatctgca ggtaactgcc cagtacgaag cagatgatcc ggatgcttcc aaatatacgg
    11041 taactttcct tgactgggat gacagcgtac tgaagactat gctggtagct cccggaaagg
    11101 atgcagaacc tcctattgat cctaagagag aaggatatac ctttatcgga tggaggcctg
    11161 cggttaccaa cattcaggcg aataccacga tttatgccca gtatgagaag aatgattccg
    11221 aagactccaa attcacggta cgtttcattg attacgatga tacggtgctg tacgtgcaga
    11281 aggtagcgtc cggcgaagat gccattgcac cacagagccc taccagaagc ggttatacct
    11341 ttacaggatg gagacctgcc attaccggaa tcaccaagga tacggatact tatgcccagt
    11401 atgctattac cggaagccag gacgatccga acgccacagc aacaccggca ccgggaacga
    11461 cagcaacacc ggctcccggg actaacacca caacaaaact gtacacattg acagttaaga
    11521 acggttccgg ttccggaagt tatgctgccg gttcccagcc gatcatcatt gcaaatgatc
    11581 cggccaaggg catgcagttc agcaactgga cgatcgatcc atccaatacc actatagcaa
    11641 gtaaggtatt atcggcaacg gtaatcacca tgcctgcggg aaatgtaacg gttacggcaa
    11701 actatacaaa gtcaaacggt tctaatacca gtacttctac cggaagtggt aattctacga
    11761 attccaattc caacagaagg ccgaacagta ctaccacagg taatgtttcc ggcggcagaa
    11821 ccactgttgt tattgacaag aacggtctgt ccaataccgg tgtggtatct gcaacggtaa
    11881 acggatcttc cgataacttt gtgatcaaga tcactgagag cgcatctgct tcagaggaag
    11941 tagtaaaggc gttgatggca gagtatggca acgatatcag tgccatcaag tatttcccga
    12001 tggatatcag tctctatgat tccaccggca ataacaagat cacggatacc acaggattgt
    12061 ccatcagcat taccctgccg attccggatt ctctgatcac ctatgcaggt aacaacaagg
    12121 tagcaggagt ggtgaacagt aagttagaca agctgacacc gaagttctcc acgatttccg
    12181 gagtatcctg cattaccttt acggcggagc atttttcacc gtatgtgatc tatgtggata
    12241 cgaacaacct gacagccgga acaatcacag acagcactcc taagaccgga gatgccattc
    12301 atccgaagtg gttcctgtct ctggggctag catgtctgtc catagtattc ttcatgaaga
    12361 aggataagaa gactctgcgc aaggcaagag cctagtgaaa ataaaggatg ggaaggttta
    12421 tgaatcataa aaagtgggta gttgcattgg cattgatagc taccgcattg atcctgacac
    12481 atcttcctgt gtcggaggca gatgcggcag cgtctgcctc cgattttcag attgaaggca
    12541 gtacactgac aaaatacaga ggaagcgatg aacgtgtcac gattcccgat acagtggaag
    12601 tcgtgggtga gagtgccttt gagaacaatc agaaggtaca gtttgttgtg gttcccaaat
    12661 ctgtgaaacg actggatgcc tatgtgttct ggggctgtaa tcatctggaa gaggttgtac
    12721 ttggcaaagg tcttacctca gtggatgaat atgctttttc aggatgcacc ggactgaaac
    12781 agatcacgat ccctgagaat gtacagagta ttgatgcaca ggcatttgcc ggatgtacga
    12841 accttacgga tatttatatt ccggacacgg tgacaagcat cgcagacgat gcattcttaa
    12901 attgtgataa cgtaacgatt catgcggacg aaggcagcgt ggctgaaaag tttgcacaga
    12961 agctggcaga gcagaaaagt cagagtcctc tggtaacggc agcgccgacg gcgacaccgg
    13021 cggcagacgg aacttcagat tcacaggcaa caacagagcc tgcccccact gaaacgccga
    13081 cccctgcacc gacaccggtg cccggaaatg tgatcggcag tacctttgtc gtaggaaatc
    13141 atgcgatggt tatgatgctg cctgaagaag aaactgtgca acagggctat acggaaccgg
    13201 tagcaggaca ggagaccgga ggagaaacgc aggatatcac tgcggaggca gaagacggta
    13261 cgattccgga gtggaaatat tatcgtgacc agtcgatcaa agcagtgagt ataccagaag
    13321 gaaccacgga aatcggacgc tttgcttttt ccagaagttc ggcagaaagt gtgacgttcc
    13381 cggaaggtgt gaccactatt gattatgcag cattttatca ctgtgatgat ctgaatagtg
    13441 tgatattgcc agatacggtg acccgggtgg aagccaaggc atttacgcat accggatgga
    13501 tggatgattt tgaagaaaac agcatggatg attacctgat cagcggggac attctggtgg
    13561 cttacaaggg ggaccttccc gaagtgacca taccggatgg tgttcgcgtg atcgctgatg
    13621 aagcattccg cagtcatacc gaactgaaaa aggtgcattt gcctgcaagt gtcaccaata
    13681 tcggtgacag cgcattcccg gagggaatcg aaattataaa tgaataaggg tcagatttta
    13741 ccgacaccag gataagaaac cggcataaga tagaataagt cttatgccgg ttttgtctta
    13801 ttttacgggg aaggaacaag atgcagcggg taagggaaca ggtacaggtg ccggagcaat
    13861 tgcatagtgg ggcaggacgt gtgaaatctg tgactgttgc tgtgctggat accggaattg
    13921 cttatcatcc ggatctggta ggacggctgc ttgatttttc cgactttgta gaaggccgtt
    13981 cctttccgta tgacgataat ggacacggga cacatgtctg cgggattgta tgcggaagcg
    14041 gagaactgtc cggaggaaga tttcgcggaa tggcaccgga ggcaaagctt gtggtgggaa
    14101 aagtgctgga cagacagggg gagggctcct gtgattccat gcaggaagct ctggaatggg
    14161 tactgcgggt gaaaaacagg tatggaatcc gtgttctgaa tatctctgtg gggatcggag
    14221 acctgaagga acgttacaag gagcagatgt tgcgaaaatc tctggaattg ttgtgggatc
    14281 ataatattct cgtggtctgc gctgcaggta ataccggtcc ggaggacggt agtatttcgg
    14341 agatggccag tagcagaaag gttctgacgg tgggttgtca tgacggaaga tattgtaaaa
    14401 atgatcccgg aaggtgtgag acgtactccg gcaggggcag aagatatgat gtgacccgta
    14461 aaccggatat tgtggctccg gggacaagga ttctgtcctg taatgctttc tggcagaacc
    14521 ggaacggcag aatctacaga ccctatgtag caaaaagcgg cacatccatg gcaacaccca
    14581 ttgtatccgg ggcggcagcc cttgctatgg aaaaatatcc gggaattacc aatgaagaat
    14641 ttgttcgtct gctgtccctt acagctaccg atctgggaga gccgtggaac aagcagggct
    14701 tcggcatgct taatgtacgg agattattag aaaacaagta aaaggataaa atcactggga
    14761 aaaataaaaa atatgattaa aataagtcaa taaaataaat accaaagata ttgacacaaa
    14821 tggtatgatt gtatacaatg atacgagaac tgcaaaatgt atttttactg cgcagagtaa
    14881 aaattataaa atcttgcaga tatcgaatgt cagggaagag aggtatacca atgagagaaa
    14941 gtgatgcatt tcagaatcgt ccggtctcca tgaatccgaa gaacaacctt ttggagattg
    15001 aagagcatat gtacatactg gatgatgtgg agaagccgaa cgtgttccgt aacatgttcc
    15061 cttattccga aatccccaaa atacctttta atgatcgtat cgtgcctcat aacatgccaa
    15121 aggatatctg gatcacagat actaccttca gagacggaca gcagtccaga gcaccttata
    15181 ccacagagca gatcgtaaag atctatgact atctgcatga actgggtggc cccaacggaa
    15241 tgatccgtgc cagtgaattt ttcctgtaca gcaagaagga cagagacgct gtttataaat
    15301 gtatggagag gggatacaag ttccccaaag ttaccagctg gatccgtgcc agcaaggagg
    15361 actttaagct ggtaaaagag atcggaatga aggagactgg cattctggtc agctgttccg
    15421 attatcatat tttcctgaaa ttaaaaatga ccagaagaca ggccatggat cactatttaa
    15481 gcgttatccg tgactgcctg gaggagggta tcagccccag atgtcatctg gaggatatca
    15541 ccagagcgga tatctacggt tatgtggttc cgttctgcct ggaactgatg aaactgatga
    15601 aggagtatca gatccccatc aaggtccggg cctgcgatac catgggatac ggtgtcaact
    15661 atcccggtgc cgttattccc cgtagtgtac agggtatcat ttacgcaatc catacccatg
    15721 caggtgtgcc gcatgagctg atcgaatggc atggtcacaa tgacttttac aaggcggtgt
    15781 ccaattccac cacagcatgg ttgtacggct gttccggtgt aaatacctca ctgtttggta
    15841 tcggtgagcg taccggtaat acaccgctgg aggcaatggt attcgaatat gcacagttga
    15901 agggtgatct gaacggtatg aatacccgtg tcatcacgga gctggcagag tactatgaga
    15961 aggagatcgg ctatcagatc ccgcccagaa ccccgtttgt cggcaaaaat ttcaacgtga
    16021 ccagagccgg tatccatgcg gacggtctgc ttaagaacga agagatctac aacattttcg
    16081 ataccgagaa attcctgaat cgtccggtat tgtgcgctat ttccaacacc tccggtctgg
    16141 caggtatcgc acactggctg aacacctatt ataagttaaa gggagacaag caggtacaga
    16201 agacttccga actggtagcg gagatcaaaa agtgggtaga cgaggagtac gccggtggtc
    16261 gtgttaccgt gcttacggat gaagagattg tggcatgtgt caatcggatc tgtctggaga
    16321 agaatctgaa gatcggcgag taagtataga atgagcatga caacggtaac agcgttgtct
    16381 ggaatcgagg attatagtga gtaagtatga tgtaaagcag gaagtaacgg acaagttctc
    16441 cctgcgggga cgggtatttc acaagctgag agaagacatc ttaagcggaa aatatcagga
    16501 acatgaagaa ttgaaggaag tggctatcgg cgaggagatg ggagtcagca gaacccccgt
    16561 cagagaggcg ttccgtcagc tggagctgga gggactgatt cagatcatcc ccaacaaggg
    16621 agcctatgtc accggtatta ctcagaagga tgtgaaggat atttacatga tccgttccct
    16681 gctggaaggt ctgtgtgccc gttgggctac ggaacatatc accaaggagc agatggaaga
    16741 gatggaagag aatgtctatc tgtccaagtt ccatgcccag aaggggcatc tggagcagct
    16801 ggcggagtta gataatcgtt tccatgatat tctgtatgag gcatgtgaca gcaagatgct
    16861 ggagcatcag ctgaaggact tccatcagta tgtcctgcgt gtccgcaaga aaacattggc
    16921 cagtgccaac cgcggtccca aatccaatga agagcatgag cagatcatgg aggccatcaa
    16981 ggcaggcaat gccgatctgg cagagcaact ggcacaccag cacatgatca acgcctatga
    17041 caacatggtg aaaaacggtc tgaacgaggc ctatgcacag caggataagc cgcaggaata
    17101 ggttgcctgt ctgtgaaaat tactgtagaa tgtaattatt cagagccaat gggcgaagta
    17161 actatatgag taaataacat gcaggcatgt tattcggtga agccttgcag aagcgatttt
    17221 gccaatgtgg ttctggatag ctattgcaaa gtaaaagaat aaataccgga aaggaaatca
    17281 ttatggaaaa gatcaaaatg acaacccctc tcgtagagat ggacggagac gaaatgacca
    17341 gaatcctctg gcagatgatc aaggatgaac tgttactgcc ttacatcgat ctgaagacag
    17401 agtactatga tcttggactg gagcatcgta atgagaccga tgatcaggta accgtggatt
    17461 ctgccaatgc cacattgaag tacggcgttg ctgtaaaatg tgcgaccatt acccccaacg
    17521 ctgccagaat gacagagtat aatctgaagg aaatgtggaa gagtcccaac ggaaccatcc
    17581 gtgcgatctt agacggtact gtattccgtg caccgattct ggtaaagggt atcgttcctt
    17641 atgtcaagaa ctggaccaag cccattacca ttgcccgtca tgcttacggc gatgtgtata
    17701 aaaatactga gatcaaggta cccggccccg gtaaggcaga actggtattt actgcagcag
    17761 acggcactga gacccgtgaa ctgatccata attttgatgg agcaggtatc attcagggta
    17821 tccacaatac gaataagtct atcgagagct ttgcaagagc atgcttcagc tatgccgtag
    17881 atacgaagca ggatctgtgg ttctccacca aggacaccat ttccaaaaaa tacgatcata
    17941 ccttcaagga tattttccag gagatctatg ataacgagta caaggcaaaa ttcgaggaac
    18001 tgggcattac atatttctat accctgatcg atgatgccgt agcccgtgtc atccgttctg
    18061 agggcggatt catctgggcc tgtaagaact atgatggtga tgtaatgtcc gatatggtag
    18121 ctaccgctta cggcgatctg tccatgatga cttccgtact ggtatctccc agcggtgtct
    18181 atgagtatga ggctgctcac ggaaccgtac agcgtcatta ctacaagcac ttaaagggcg
    18241 aagagacctc caccaactcc atcgctacca tctacgcatg gaccggagct ctcagaaagc
    18301 gcggtgaact ggatcagatc cccgagctgt gcaacttcgc agacaagctg gagcaggcat
    18361 gcatcaagac catcgaggat ggcaagatga ccaagagcct gtccctgatc tccacctgtg
    18421 agcatcccgt gatcctcaac agcctggact tcatcaaggc tatcagagag acactggaca
    18481 gcctgctgta aattgagagt tagtgaaata atcccgtagc ccttggcagg gtataagaaa
    18541 cgcggca
//
LOCUS       DAWEJW010000057        18474 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1143_length_18474_cov_64.363755, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000057 DAWEJW010000000
VERSION     DAWEJW010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 18474)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18474)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18474
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1143_length_18474_cov_64.363755"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11208..11307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15988..16087
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4385 a         5112 c         3950 g         4827 t
ORIGIN      
        1 tagttgcatg cactcttgaa ctgattccag atctccgcat gcagatcctc tgcctcctgg
       61 gagatattct gcacgatctc gccctccgct gcatcttccg gcaatacgat cattttcttc
      121 atgtcgtcgg aaatatactc ttccgcagct ttgttggtgc agtagtagcc gatatactca
      181 tagcatttcg ccgcattctc aggatcgttg atatagtcca aaaatgcata agccgcatct
      241 gcattgggtg cctgggaagg tataaatccg gccatgatgc cgaagcccag tccctccttg
      301 ggatatacca cttccagatc cggtctcgct tgcagtgccg cagatacctg ggaagtatac
      361 aggaacgctg ctgccacctc accgctgatc agataatcct gcgtattatt gtcattgatc
      421 acacggatat tgggagccag actcagaagc ttctctcctg ctgcccggat cacatccaga
      481 tcctcggtat tgaaggattc tcccatggtc tttaaggtga tgccgtcgat aacacgatag
      541 tttgccgtca gtgccacgtt attttccagt gccggattcc acagatcctc atagcctgtg
      601 atcttcacat ccgtcagtcc cggatcgtat acgatcagag gaatgcccgc tccgtagggc
      661 acggtatatt cgttcttcgg gtcatagaac tgagacataa atacaggatt caggttatca
      721 taagtactga tcctggaagt atccagcttc tgagccaggc tctcctgaat ggccagctca
      781 atgatatagt catccgcaat gaccagatcg taatcactgc ccctggtttc ttccagcttc
      841 gccagcatat cctcatcata gtcaaaatta gaataattga tacggatacc ggtctctttt
      901 tcaaaaccat ctaaaatctc ctgggggaac atgccgtccc aggtatagag attcagttct
      961 cccttatctt ctttaccgcc tgctccacag ccggtaaaca acgtggccag catggtcgct
     1021 gccaacagca ttgccagctt tttcttcata atctcctcct tcgatacagt tctcctgcat
     1081 catctgctat acatttttat gttgttactt atccgcaatt gcatttgcgg aatcgttctt
     1141 tcctcatttt cccccggcag ctttctctac agctgtctgc gacttacgtc ccagcacata
     1201 cgcccccgcc agacacaaca atatcacaaa cagcatgacc gtggcaagtg cattgatctc
     1261 cggtgtcaca ccggtcttta atttcgtgta gatcttcacc ggcagggtat tgaccttcgg
     1321 tccagtgaca aaaatactga tcaccacatc gtcgaaggac atggcaaatg caagcagcac
     1381 tcccgacagg atcgccggaa gaatctgtgg caggatcaca tccctaaata cctgccactt
     1441 cgtagctccc agatccagcg ccgcctcttc cagagaccgg tccattccca ccagtctcgc
     1501 ccgtaccata gtgaacacgt agggcacaca gaacgtggta tgggcgatca ccagcgtgac
     1561 cataccaaag ggcagtccca tcagggaaaa tacggacaaa aataccatgc ccagaatgat
     1621 ctccgggatc atgatgggga gtgtggacac ataggccacg gcatcgtcca gtttccgttt
     1681 tctgcggctc atccccagtg ctcccgaagt gccgatcaac accgcaaagg cacagctaag
     1741 taccgcaagg atcaggctgt taatcagtgc ttcccccaga tctctgtccc ggaacaactc
     1801 ccggtaccat ttcagggaaa agccctccca cacagaggtc agtttggaag cattgaagga
     1861 atacaccacc gtcagcagga tcgggatata ggtcagcact gtgatcaccg ccacatatac
     1921 aaagggccat tttttctttt tcatacgcta catcatccct tccagctgtg aggttccggt
     1981 gatcttgcgg tacagacgga tcatcacgga agtcagtatc atcaacaccg cagacagtgc
     2041 cgccgcaaaa ggctggttcc gccccttggt gatctgttcc tgaatcacac tgcccaccag
     2101 cacgatctta ttgccgccca ggatatccgc tatgaaaaac agtcccatgg acggaataaa
     2161 ggacagcacc acgccggaca aaagtcccgg cagcgtcagc cggaagctga cggtccagaa
     2221 ggctttccac gccgaggctc ccagatctct ggaggcttcc accagagagg catccagctt
     2281 ctccgcactg gaatacaccg ccaggatcat aaaaggcagt agcgaataaa ccattcccac
     2341 taccactgcc ggataagtgt aaagcagctt taacggctgc ttcgtcaggt gtagccccat
     2401 caggatctta tccagtacac cattgctgcg aaagacaata atccagccgt acaggcggat
     2461 cagcgcactg gtccagaaag ggatcataat gagcaacagc atcctgcgct tccagacagc
     2521 gttcattttt gccatgaaat agccgtacgg atatccgatc agcgccacga aaaaggtggc
     2581 ggtaaatgcc agcttcagag actgccagaa agtatccaga tacagcggtt ccaatattct
     2641 tttgtaattg tccaacgtga acgtaaagtc cacaccccag acctgtgctc tctgcaaaaa
     2701 gcttaacaga aacagataca ggatcggcag agccacgaac agaatcgtga acacatacat
     2761 agggatcatc agtccgacat tcccgcttct cctgcggctg ttacttgtga ttttcgtctt
     2821 tgtcattatc tattaccttt actgcgtctt tcgcatccca gccgatcttc accttctgtc
     2881 ccggctgtaa gtcattgtcg attccatagc ggctggcggt cagagtctgt ccgtccggca
     2941 gacggaacaa aatgcgtaac tgccctccgg caaagctttt ttccaccacc acagcttccc
     3001 gggtgcagac agtctctccg ttcatcagaa tgttttccga gcggatggca aaagtctccc
     3061 catttttatg cagaatattc gcatttccca caaaatccgc cacatagctg gtctgcggct
     3121 gatagtacac ctcattgggc gtccccagct gttccaggat gccttcgtgc atgacaccga
     3181 tgcggtccga catattgatg gcttcttcct gatcgtgagt gatgtaaata aacgtgatcc
     3241 ccagctgctt ctgcagacgt ttcagttcca gctgcatctg cctgcgcagc ttcaggtcca
     3301 gtgctcccag cggttcatct aacagcaata ctttcggctc attgatcacc gctctggcaa
     3361 tggcaatacg ctgcttctgt ccaccggaca gctgatccgg catccgtttt ccataccctt
     3421 ccagctgtac cagacgaagg gcctgctcca ctctctctgc aatctctgcc ttgggaactt
     3481 ttcggatctt caatccatat cctacgttgt ccgacacatt catatgcgga aacagggcat
     3541 aattctggaa caccgtattg acatcccgtt tgttcggctc acggttcgta atgtcctgtc
     3601 cttccaggat cacctgcccg gcatccggca gctccaaccc tgcaataatg cgtaaggtcg
     3661 tggtcttgcc acagccagag gcgcctaaca gcgtaataaa ttctccctgt ccggcttcta
     3721 agtcaatgcc ctttaatact tctgtattat caaaagattt ccggatctgc cgaagttgta
     3781 atatactttc gcccatgcac cggctccttt cctcattttc atatatcatt tttgatacag
     3841 attctaccgt atttttttat ctcagtcaat cctttatccc caggcaataa aaacgataaa
     3901 aacaggttgc cgacttcagc gccgccagaa tatcaggcat taaaaaaatg ccaagccaca
     3961 ggttttcatc ctgggctcag caccatattt tacaactcaa caacccggtc attcacctgt
     4021 gtaaagctgc ctccgtccat atttttacgt acaaagggaa cccccctgcg cacatagtat
     4081 acattatcct gaagcagggc atccggattc tctgacgggc tgatcgtacc accgagatca
     4141 tcatagctga tattatcgcg gaaggtatta tggatcgcct cctgcagaca gaacatcaca
     4201 cagccgcctt cattctgcct gctgtaattg gattcataca ccgttccgtc cccggaatcc
     4261 gcatcatatg ccatgccgtc ctgattgagt ctggtgtccg tgacttcatt ataacgaaac
     4321 agggcatcct tgcatttcca gggccagatc ccggctgcca cttttcccat ccggtcaccc
     4381 ggctcgcaat agatccggtc attgatctcg caggccacag agtctgccat attatgctcc
     4441 accagaggac gcagggcata catcggcgtg atcccatccc caccggcctc ttttacataa
     4501 ttattccgta tctgcatatt ttcatgtcca tagttcagaa aaacggcttc cgtaagctct
     4561 gccccccgga acttatcatg ggcataggta tagcctgctg caattcccca gcggctgacc
     4621 cggtgcacga agcatccttc aataacgata tcccgatacc gtgccacacc cgtcagctcc
     4681 tcacattccg gacggagcgc tgtcatatag atcccaccgt tgttcatgtg tttatcgtag
     4741 acattcccat ggacatcatg gatccacaga ttcctaagtg tgatgccgcc ccgcacccct
     4801 ttgtccttag ccaccacagc cacaccggta cggttcattt tctcacccag actataacgc
     4861 tctcccggga tcatgctgcc ttcattcgtg atctccagat cctcgactac aatatactcc
     4921 gcatcgtaca aaagcactgc ggaagataca tagccgtgat acacatgcgt aggtgcatcc
     4981 agcggggtcc cataatcctg ataccagatc ccctgtccgc aggcatcgat ccgcggcggt
     5041 gcatctgctg tgccgtacgc tccgatcacg atcggtgcct ctttcgtacc gctgtctgtc
     5101 acatgcagat attgtccgta aaatacactg ccccgctcca gcaggacacg gtcgcccggc
     5161 tgcaggcttc ttcgattaat ggcagacaga ctggcaaacg ccgtactgca cgtccgtccg
     5221 tcattttcat cactgcctgt tctctgactc acataatatg tcgcggataa gcttgtcata
     5281 tgcatcctcc cgtaaccgtt tctcaatgat ctgtgacacc tcctcatagg taaaatagtc
     5341 cccatgatag cgtccgaaca gatctctgtt ctgttcgtaa aaatcccgct tctcctgctc
     5401 cgtgaaatcc gggcagaagc caccttcccg gaccttctca cacggctgca caaaatccag
     5461 cttcaggcgg gtcagctcat attgcagata cgccggtgcg gaaaaataat gcggtccgta
     5521 gaaccgctgt ccttcctgtt ccattctccg cagctcttcg ttgtgcttct gtcgtctggc
     5581 ctttagtccc tccatggagg gatcctcgat caggcccatt ctatgcgcca aaaggaaggc
     5641 tccctgagcc ttcctgatat gcgcctgtgt catagtttcc tgttttggca ttttctgttg
     5701 cattatttca ctgctcccgt aattccattt gcaaaactct tctgtaaaca caggaagatg
     5761 atcgctgtag gaagtgtaca gatcaatact cccagcatca gcataccata atccacactg
     5821 tatccactct tcagattggc tactaacatc ggcatggtga tcgccttgct gtcctgtaaa
     5881 atgatcgtcg gccacagata attgttccac gcattcataa atgtaaccgt catcgccgct
     5941 ccataggtag atttcatcac cggaatgaac atccggaaga aaatctgtat ttcgctcagg
     6001 ccttccagcc tggcagcctc aataatatca tgagggaaag aacgtgccgc ctgtctgaaa
     6061 agcatgatca taaacggagt cgagatggat ggcagtgcca gtgcgatcca ggtatttacc
     6121 agcttccagg aggtaaacat cttgaacaga gggatcatga tcgctacgaa cggaagcatc
     6181 attgccagca gcagaatact catcagaata tccttgccct tatcatgata gatctcaaat
     6241 ccataaccgg caatggaaca gatcagcagt gtcacaaccg tgatcacgat cgcattgcgg
     6301 aaggaattaa acatcgcaag tccgagattc tgcatgtcga ccagggtctt gaaattctcg
     6361 atcatatagg tacccgggat caagcgtcct cttaccacat caatactctg gttggtcgca
     6421 ccgcacagca tataatacag cggaaatgct gacagaaagc ttgctatcac taagatcgcg
     6481 taggaaatta tttgcttgaa tttagtcacg tttgtcaccc accttcatct gaattgcaga
     6541 cagaaccgct accatcacaa gtatgcatac agacaacgct gccgcataat taaatttcgg
     6601 tgtctccaca aaggagatat tgtaaatgta atgcgccaat gtcattgtag attttccggg
     6661 accgcctgct gtcaggttca tagactcatc gaacagctgc agtgttcccg aagtagacat
     6721 gatcgccgtc atcagaatgg tcggctttaa caacggtatc gtgatcttca taaactgctg
     6781 gaacggtgtc gctccgtcga tgtagcttgc ctcatataca gaatagtcga tattctgtaa
     6841 tcctgccaga tagaatacca tattatatcc ggtccatctc cagatcagtg cgatcacgat
     6901 gacccatcgc gccggccatg tgctctggaa ccagtccacc gaagacagtc cgatggccat
     6961 cagcactctg ttgaccactc catccggagc aaacaatgac ttaaatatca tggaataaga
     7021 taccagtgac gttgcacacg gaaggaagat caaagtacgg aaaatacctt ttccttttat
     7081 cttaggatta ttcagcagct gcgccagcat cagtgcgagc acaagcatga tcggtacctg
     7141 aatcacaaaa taaaagaagg tattgaacaa gcattgctgg aatgttttat ccttgaagat
     7201 cctggcataa ttcgctacac cattaaagac tcctcctgtc ttccggaaag aaagaaggat
     7261 ggcctgtgac atcggataga aacaaaatac aaatattaaa atcgatgccg ggagcaggaa
     7321 tgcccatccg gtcagattat gccttttttc caaacttaat tttgctttct tcttttccat
     7381 gtttctctcc tacaacagta ccgggaacgc gctgctgcac ccccggtact atccagtttc
     7441 catactctgg tgattattcg gcgatattaa actctacggt atcctgagcg tttttgatct
     7501 ctgtatcgat atcagcgttc ttctggacaa cgttggtgat tgcatctgtc agtgcgcttc
     7561 tgatgtcaga gtagtatgca ccgtagtcaa ttccgggaac atgtccggcg aactctacga
     7621 tatccttgta cactgcctga ccgccgtaga actcattgcc ctcattatag gtctcagact
     7681 gtgctgcggg caggtaactt gcgatcgctc ctgcatttac cagcagatca tcgtataaat
     7741 ctacatccgc accaaaggta gcgttcagga aatcaaatgc cagatcggta tttttgcagt
     7801 tagaagatac tgcccagctt gcaccaccgc agttagcgta gttggttgcg ccttctacat
     7861 catctaactt aggcatattc acgattgccc atttacctgc ctgatcctct gctgcctgaa
     7921 tggaggacat gatccagcag ccctggataa cgcctgcagc agtacccttg ttcatggaag
     7981 cgatatactg atcccagtcg gtataatctg ccatgattcc ttcatcgatc agctgcttat
     8041 aggtctcgat tgctttcttc agtgctgcgt tgtcaaccag tgtcaccttt ccatccacca
     8101 tgggagaagc gcctgcagac tgtaacatct cgatcacaat ctcagaaccg ccggagcagg
     8161 tcagcatggg aacatcattc gcagccatta ccttcttgga cagctccatg aactcagacc
     8221 atgtggtatc cttgaaatct tctaccttaa ggcctgctgc ttctaccata tcggaacgga
     8281 tcgccatgat ggtagcacca ttatcaaaag gaataccata atgtgttcca tctacagtgg
     8341 agtcagccag cttacccatg gaaaactggg agaaatcaat tccgcagtca tccagtgctg
     8401 tgaagattcc gggataattg gttgcatttt tgtggaagga ataatcctgc atcaggaaga
     8461 tatcaggtaa agtgctgtaa tcaccggctt cagctgctgt gatcagagca gtctcgatat
     8521 cgctatatac cttctcctga atgtctaact taaagttagg atgatccacc tggtatgcct
     8581 tctctgcctg cttcattgca tagacattga agttagggtc ccaacaccat acagtcagtg
     8641 tctcgtcttc tgcggaagca ggtgtttctg ctgctgcctc tgtttccttg gatgcctcgc
     8701 tggatgctac gctctgcgca tcatttccgg caggcttgct gccacatccg gtcagcatgg
     8761 acatagccat tgccgctact aagagcacgc ttaatacttt ctttttcatc ttaaataacc
     8821 tccttatact tgtaaagtac taccttataa aatatgaaac gctttttgct ttccataagt
     8881 tacctgtctt ccatatcgtc cacccaacgc atttcgccgg gcttcagatc cagcacatac
     8941 gcacattttc ctttgatata cagctctgtg tgctgtgcct gtgcagagtt gttgatcact
     9001 gcgatcttgc cggtcttctg gaataccgtt acttcggtat ccacattcgt tacatagtaa
     9061 cgtttcatct cctgctccat gccggctgca taataaattg cccgaagcag aagtctgcag
     9121 ttctggggag aatacggaag acctgcaaaa tatacgctgt gtcctctgcc atattcattg
     9181 accaccagct ggctgtattc cccatccatc gcaaggatct gataatgtcc gccctgtgca
     9241 tagacgcggc tggtgccctc gccgaaatct aagcttccgt caatatcctc taagataaag
     9301 tgatcgtcat ttttctcatt atatttgtcc gtactgagag agaagcacat ctcgcggtct
     9361 acaccgagca catcggacaa ttggaaatat cttccctgat actgataagc ggtaggctca
     9421 cccacaccga tgaagccacc tccctgatcc acaaagcgtc tgatattggt cagtaccttt
     9481 tcgtcgatcc agttctccgc accgctgaat gctgtgtacg catccccgac attaatgatg
     9541 accttgattt cctcagggat gccgttcctg acatcatcaa aggaaatgaa ctcgacctca
     9601 aaaggcattc cgcttaagca ttccagtact ccctctgcgg aataagtttc cctgtgccag
     9661 atcgcatgat gtacctgatt ggacatccac cttctctgac tgccccagca gttcaggatc
     9721 gccaccttga aaggtgccac ataggaagct gtcccctgca tattttcatg gatctcccga
     9781 aactgcgtta ctacattttg tatttcatca atgaaaccgg gccaattact tgccagcttc
     9841 agatagccgc catatccaat cctgtccagg ggagacctga gtaatgcacg ccttgctttt
     9901 ctccagttat cccttgcctc tccgatcggg tcaccaccct cgcagaacac atccgggaaa
     9961 aagtaaggta acagccgtcc ttcggtataa tccactccct tgatatcaga gatcatgcgg
    10021 agtgtcacac cgcttccgac agagcctacc actgcatcca gtccgatgcc tgcgaaatat
    10081 ttaccgtagg gctctgttcc gatccaatga tctccgagga acatcatcgc ttccttgcca
    10141 taagaatgta caatgtctac cagctccttc gccagcgcac atacctcgat ctgctggaac
    10201 tctatgaaat ccaggaactg tttgctggga acgcggaaca tgctgttgtg atatccctga
    10261 tccacaatgt actccgggcg aaatttgtag cccgcccatt tctcaaactg ttccaggata
    10321 taggggctca cacttgcact gtatccgaac cactctacaa atttttcccg cttcttgtca
    10381 tcgaaggtca gtgtaaactg atggaaaaag gtagtgaagc ggaccacatc gatatgagga
    10441 ttgtcttcac accattttct cagcttgtct ttgacatact gtttggtctt gggctgtctc
    10501 acatcgtaag tgagctgatg cggtgtatcc ttccagtcat tcgtgatgaa attgtacatg
    10561 tgtaccggat cccagatcaa aaatgccaga aagcttaccg tatactcatg ataggggatc
    10621 gtacggatct ccacttcacc ggatgcctca tcaaattccc atgcatccgt cggcaccact
    10681 tcaccggtcg ttctgtcgat cacttcccac caacgcttcg gatcgtccag cgtattgacc
    10741 ttaagctgct ccgtatggaa gccttccatc agacggatgc ggagtgtttc ccctctggca
    10801 gtgatgcggt tgctgatcag gtattcctgc tggatctcct ccgggtgctt catcgcccac
    10861 tcattatcct ttctcgtggt ataataagtt gcgtatttct tcaccggctc tgacaaaagt
    10921 gcttccggca tttcggttcc gtcacagtct cttaaagcgt ctgcacccag aagatttttt
    10981 aagcgtatcg tttcgtcaat gatgtctgca tcggtcggca gcgtcagtct tcctgtcatc
    11041 gcatggtttc ctcccatcac ttttgtacta tcttgcagct gatgactaaa gtatatcggc
    11101 ttcctgacct gatatcaatt tctttgttgc ttgttttgtt atgtttattg ctattttgtt
    11161 actaaaatag tggatttttt gaaatgatta tgtataatat gcttatcnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnctt cccaacgatc ccatggaata tatcgtattc
    11341 ggtgtggacg gacttgcatt ttctttttcc ggtgcagggc aggacaatcc gaagggatac
    11401 agcttctata gctacggctc tgacaaaaaa cagttcatta acttcgccca gctgatgatg
    11461 caggaattcc gcgacaagct tcccggcttt gaacaggtgt gccacggact gctccaagtg
    11521 cttctggtat atatttcccg gaagcagaat ctgtccgtca tctctgactc ctccttccag
    11581 ttgtccaagg aatgtgctct ggcgaagcgt tatatcgatt ccaactattc ccagaacatt
    11641 acactggatt ccctggcgga gatcacacat atcaacaaat tctatctggc acactccttt
    11701 accgaatgtg tcggacagtc tccgatcaat tacctgacag accggaggct ggaagcctgc
    11761 aaggggcttc tggtatcctc caatctgtcg gtagctcagg tagccaccag cgtcggcttt
    11821 tcttcgcagt cttatttctc gcagatcttc cgtaaaaaga ccggcatgac accgcgccag
    11881 taccggagcc ggtatgccag aaaataatgt gtacactcat aaaagacctc actgccggcg
    11941 catctgccga ctgtgaggtc ttttataatg gacatttatt cacttatttg atccagcccc
    12001 agcataatcc cagatattcc accagaaaat acatcccccg cagccatatc agcacggcac
    12061 agccatagaa catccagaca ttttctttct gcctgtctgc gattttccct gccatggata
    12121 aaatcgcaca aatcaacaaa accatcgttc cgaaagccgc ccggtctgga tagtgtgggg
    12181 acaggatcat ggcaccccag gacaacagtg cccccagcaa taacagcact gtatttctgc
    12241 ccaggttttc tttcagaatg cctttgcaga cgatcagtgc aaataccgta agcagcaggg
    12301 cagggaacag atactccata actcccttcg cttctgcgta gcagcgcaga tacattctcc
    12361 acagcaggct gtaggcttct tcccccgtct ccccgctgcg gacaaaattg cccggtgcag
    12421 tgatcatcag aatggttccc gccaggcagc tgatgtttcc cagatacatc cacaccggag
    12481 cttttttgtg ctctctgtgt cttaacagga tcaccagaag actcaggagc cataccgcag
    12541 gccccatatt ctcattgctc cagcctgcga tcagtcccag tggaaggatc caaatgacga
    12601 tgccgggaag atttttttcc tcatatttca ggtagcagaa caaaaatgcc agaagaaatc
    12661 ctgtcatgta cagataattc gctgcacctg cttcccagaa catgctcatt ttccagttgg
    12721 cattcaatcc caggatcatc cccaacgctg caaaccagta cgggagctta cgtctgtctg
    12781 ctatcatgca ggccagccat cccaacagca gtgtcattcc tgtattcagg atatccgccc
    12841 agtgttctcc ggccagcagg atcagctgca gcagtgcatg cgccatgctt ctgccgcccc
    12901 agttaaaata atgccagatc tgggcatgga taatgtcact gagattccgg atcggtgtat
    12961 ccgcatacaa taccgtcgaa taccactcat catccatcat aaattcgatc tcatagttga
    13021 cgcgccacac gatcagtgct gtcaatgcga tcagcaccag gctgatccac cggattgctt
    13081 ttttctgctt ttcctgcata tatctgctcc attcctctta tggcaaagaa cggctgccta
    13141 taactgaatc ttcttcagac gatccactgc ctccacggta ttctcatagc ttccgaaagc
    13201 cgtcagacgg aaataatgct ctccgctggg tccgaaaccg gatccgggcg tacccaccac
    13261 gttgacattc tccagaaggt aatcgaagaa ctcccagctg gtcatgccgt taggcgcttt
    13321 cagccagata tagggagcgt tcacaccgcc ggataccgtg aagcccgctt cctgcaagcc
    13381 cttcaggata tactgtgcgt tgttcatgta ataagcaacc tgctccttca gctgtgcctt
    13441 gcctgctgca gagtatactg cctcaccggc gcgctgtacg atatagggtg ctccattgta
    13501 tttggtgccg tgtcttcttg cccacagtgc atggaggctc acatccccgc tctttaaatc
    13561 cttggggatc acggtaaagc ccagacgcac tccggtaaag ccggcattct tggagaagga
    13621 acggaactcg atggcacagg ttctggctcc ttcacattca tagatactgt gaggcacatc
    13681 cggttcggaa atatatgctt cgtatgccgc atcatagaga atgacagcgc ctaccttatt
    13741 ggcatagtcc acccactcct gcagctgcgc cttggtaatg gtggcaccgg taggattatt
    13801 ggggaagcac agatagatca gatccggggt ctccttcggc agctccggtg caaaaccgtt
    13861 ctctgccaga cagggcatat agatcacatc actccaggtc tcggacttcg gatcgtaagt
    13921 gccggttctg cccgccataa cattagaatc tacgtatacg gggtaaacgg ggtcacatac
    13981 tgcaatcttg ttgtccaggc tgaagatctc ctgaatattg ccacagtcac attttgctcc
    14041 gtccgataca aagatctcgt ccggtgcaat gtcacagcct cttgccttat aatcattctc
    14101 tgcaatggca ttacgcagga attcatagcc aaggtcggga gcgtaaccat gaaaagtctc
    14161 cgcttttccc atctcatcca ctgcaccatg cagcacatca atgatagcgg gcgccaaagg
    14221 ctgtgtcacg tcaccgatgc ccagacggat gatcttcttg tccgggtgtg ctgcggaata
    14281 cgcacttacc tttttgccga tggtggaaaa taaatagctt ccgggaagtt ttaaatagtt
    14341 gtcgtttact ttaaacatag tacgttctcc tctctattat ccctggtgca gtattgctgt
    14401 caccttgctg tcgtaaataa gcaatttttc cgcactacgt gctatcagat ggagtacggg
    14461 tccacttcac cttcaaatac gatcttcgca ggaccggtca tgtagaccag attctccttg
    14521 cggtcccatt tgatgtgcag ttcaccgcct aatagtttaa cagttatttc ctcgtccgtc
    14581 aaaccattta gtacacaagc caccgccgtg gcgcaacatc cggtaccaca ggcaagggtc
    14641 tccccggtgc ctctctccca gacgcgcata aacacatttt tccgatccag aatctctacg
    14701 aattcggtat tggtccggtt aggaaaacag gtatgatgct caaacttcgg gccgatcttc
    14761 tcaatgtcaa gatcctttac cccttccata aatacgatgg catggggatt gcccatggat
    14821 acacatgtca tgtggtattc cgtgtcgtct accatgatgg gagcattcac taccgccggt
    14881 ccgtctgccg tctcctgtgt ggcttccact gttaccggca cctctgccgg agcaaggatg
    14941 ggatttccca tgttcacctg taccagcgtc acctgtcctc tgtccgtggc ggtctttccc
    15001 tccacggtga gtttcagata ttttattttt ccggcgctga cgatggtaat gtccttcttg
    15061 tccgtcagtc ctttgtcgta gacatatttt gccacgcagc ggatgccgtt gccgcacatc
    15121 tccgaacggc tgccgtccgc attgtacatt tccatctcaa aatccgcttc ttccgccgga
    15181 ttgataaaga tcacaccgtc gccgccgatt ccgaaatgtc tgtcacttaa gcttcttacg
    15241 atctccggct tctcttccgg tgaaagcttc tccgtaaagc cgttgacata tacataatcg
    15301 ttgccgcagc cctgcatttt tgtaaatttc atggattgct gttcctcctg ttgttctctt
    15361 tcacatatcc tgtctgtaac gcccggatcc ctgtaatcat ttccgggccg tcctgcttac
    15421 tccgtcatca tggacaatac catctgtgcc gtctccacca gattggtacc actgtccata
    15481 ctggtcttct gaaggtagcg gtgggcttct tcttccgtca tatgatttcg tgccatcagt
    15541 aattccttgg catccttgat tgctttttca tccgctgcat tccgcacttt cggctgcata
    15601 cggcgttttc ttctgcggcg ttccacacct tccaccatca ggttcacggt attgatcagg
    15661 tcctgtacct ttaacggcat ggaaagacat acgatgccat tgccatagca ttcactgatc
    15721 agattctggg atgccatcag cagcatctcg aaaccttccg gcagatcttc atgtagttct
    15781 gtgtatatca tgtccgccag cttgtagctg cagatcacaa taccgtcgtt cagaccgtcc
    15841 gcctgactga tagcctgagt gcctgtggta cagatacccg tgacctgaaa gccactgcgc
    15901 accagaatat tcttgacgcc tttggcttcc tccagcttcg gcagtacgac aataatgttc
    15961 gtcatcggcc cacctccttt cttctgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnntcg gatacctgag aacggtactc atgccactct ttccgcttgg catcgatgta
    16141 ttttccggca atatgatctc caagcacttt ctgaatgaaa gtatccttct ccagttcctc
    16201 cactgcctcc agaagtgtgc cgggcagctg atcgatatgc aattcctcac gttctttttc
    16261 tgacagggca aaaatattgg cattgatgct ctgaggcggc tctattttct cccggatgcc
    16321 ctctaatcct gcactcaggc agactgccag tgtcagataa gggttagcag cagaatcggg
    16381 actacgcagc tcgatacggg tgccctctcc gtctgcagca gccggaatac ggattaaggg
    16441 gctgcggttc gtcgtgctcc atgcaatgta gaccggtgcc tcgaaaccgg gtaccagacg
    16501 cttgtaggaa ttcaccagcg gattcgtaat ggctgccatt cctttgatat gtctcatgat
    16561 accgccgatg aaatagtaag cctcttcgct cagaccattg ggatcggaag catcctggaa
    16621 aatgtttttt ccatccttct gcaaggacat attgatgtgc atgccggaac cgttgacacc
    16681 gaacttgggc ttcggcataa aagtagcgtg caatccatga cgtctcgcaa tggtgcgtac
    16741 ggccatcttg aaggtcatga tgttgtctgc ggtatttaat gcctcatcgt atctgaaatc
    16801 aatctcatgc tgggccggag ccatctcgtg atgggaagcc tcgatgacaa atcccatatc
    16861 ctccagattc atgacaatgt cacgacgggc attttcgccg aaatccaggg gaccgatgtc
    16921 gaaataccct gcctgctcat gggtgttggt ggtaggcatg gtattttcgt ccgtattaaa
    16981 taagaaaaat tcacactcgg ggcctacctc aaaggtatag cccatgtcct ctgcctgctg
    17041 caccgcacgc tttaacacat aacggggatc tccctcgaaa ggctgtccgt tgggacgata
    17101 tacatcgcag atcatacggg ccactttgcc ctgctggggt ctccagggga aaatctccag
    17161 tgtcgacaga tccggataca gatacatgtc ggattcttca atccgtgcaa aaccgtcaat
    17221 agccgaaccg tcaaacatac atttattgct caatacgcgg tccagctggc tggctgtcac
    17281 cgcaatattt ttcagattgc cgaacagatc cgtgaactgc agacggataa attccacatc
    17341 ctcttcttcc acaagacgga tgatgtcttc cttggtataa ttattcatcg ttaccctcgt
    17401 ttctgcgccg cctgtctgct ccttttccac ggggcggacg catccgtgaa aaatacttgt
    17461 cttaactata gcaatgcctg ttttgtcttg tcaatgtgaa gttctcctta tttgctgtac
    17521 tatgagtacc ggcctccgca tactatttat aaagatcatc ccatcattcc aaggagactt
    17581 aagaatgagt gcaaatacaa agatcgttgt ccttcgcaga aaagagctgc tgtataccgg
    17641 aatcttcgct gcgctgggag tgctgttcgt catattactg ttaatgttat tgctgcccgg
    17701 aaaagctgcg gatacctccg gggcaccgga ttcccctgac agtactgccg agaccgttat
    17761 gccggacaat gtggcggatc tcggacgtat cccgcaatcc gcacagacgg aggatgtctc
    17821 caccggggcc gttgccgata ccgatgctgt tctggataat gcttccgggc ctgtcggtac
    17881 tgataataca tatatcccgg ggatctacac cacggagctg atcctcggca gcgagaccgt
    17941 gaatgtagag gtcatcgtaa acgaccacgc catcacatct ttaagcctgg cagatcccga
    18001 tgagaccctg accaccatgt atccactgct ggagcccacc atggattccc tgagtgagca
    18061 gatctgcgag acgcaagatc tgagtcaggt gacgtattct gcggagacca gatatacttc
    18121 cctggtgctg ctggaggctg ttaaggcttc actggaaaaa gcaaagccca gtgccacgcc
    18181 ggaagcagcc tccaccccgg cagcttcccc ggagaattca gcggccactt caccggaaac
    18241 ggatgggaaa accgcggaac ctacgaagat gccgacagcg gatatcaatc cggcagcata
    18301 aacacttaga agctttggca cactacatcc actgtacaca aaaataaatg ctgacgcaaa
    18361 aaagggcgca tgcctcccgg catgcacccc gttgtgcgtt ttattgtaat attttgtttg
    18421 caacactgag tttattttat actatttgtg ggggagatgc aagggaaaat tatt
//
LOCUS       DAWEJW010000058        17694 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1206_length_17694_cov_64.994841, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000058 DAWEJW010000000
VERSION     DAWEJW010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 17694)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17694)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17694
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1206_length_17694_cov_64.994841"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5921..6020
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4575 a         4028 c         4750 g         4241 t
ORIGIN      
        1 gccatattgg tcatggcatt gtcggtatgc atagcctcat ccagggatac cacgttccgc
       61 agaatcggga aaaaggcatc aatgccctct gcgttgcagg caacggcctc tttggccacg
      121 cagccggaaa aagcaagtac cggtttttca tattttttgg caatcttggc aacaccgatg
      181 ggagcttttc ccataaccgt ctgaccgtca aggcggcctt ccccggtgat cacgacatct
      241 gcatcccgga tatatttttc cagatgggtt tcttccagaa tgatcttaat accggattcc
      301 agtgtggcat tgcagaaggt aagaaaagca aatcccatac cgccggcagc accggcaccg
      361 ggatatttgg catcggcatg aggaaactgt tctctggcca gagcggcata ataggccagc
      421 catttgtcca tcagcaggat catagtggaa gtagcgccct tttgcggtcc gtagatggcg
      481 gaacatccct gctctccgca gagcgggttg gagacatcgc aggctatgcg gaaagtacat
      541 tccttgagtt cgggaagcac atgatccgta gtgatggatt ccagatcctc cagtcctttg
      601 gctccgaagc ggatctgatt tccgttttta tccagaaagc caaagccaag tgcctgcagc
      661 attccgacac cgccgtcatt ggtggcactg ccaccgatac cgacaataaa atgccggcag
      721 ccgttctgga tcgcatcttt gatgacttcg cctacaccgt aagtggtggt gttcagagga
      781 ttcttttcct ctccgctgac ctgggtaatc ccggcggcac cggacatttc aatgattgcg
      841 gtatgggtct cgtcgacaat gccgtaagga cagacaacgg ggtgaccgga agggcctgtg
      901 acctgcacct gtgtcatacg gctgtcacag ccgcttacca gagcatctac ggtgccctcc
      961 ccgccgtccg ccagaggacg gacggtaata tgtgcatctt tataaacacg tttgatcccc
     1021 gtggcggcgc tcaaacccgc ctgcatggag gtcatacttc ccttgaagga atcgatagct
     1081 attactacat tcataaggtt cctttccgct gccgccggaa ttcggccgac aactgtcgtt
     1141 ttcgtataga ttttcggaaa atggcataat ttctgaggat attatagcaa caccggaaca
     1201 aaagaggaat ggttcccgga gaaaaataaa atttagcgtg gtacagtggc aaaaacatcc
     1261 tataagatca ttgcatctga gaaaacaatc cagtataatg aaaggagtgc acatttagat
     1321 gcacattacg cataagaagt ggaataacag gaggacgagc atgattcagt taacacaggg
     1381 cggcgcttac ctggtaaacg gtacggacat cgtagcggat accccggaag cagccagtca
     1441 gatacaggcg aagaccggca ttacgatctc caaagaagaa gcagcgaaaa acaccatggc
     1501 atatggtatt ctgcaggagc ataatacctc cggcaatatg gacaagctga agatccgttt
     1561 cgacaagctt accagccatg atattacctt tgtaggtatt atccagactg cgagagcatc
     1621 cggactgcag aagttcccca tgccctatgt actgaccaac tgtcataact ctctgtgtgc
     1681 cgtaggcggt accatcaacg aggatgacca catgtttggt ctgacctgtg cgaaaaaata
     1741 cggcggagtc tatgtacccc ctcatcaggc agttatccat cagtttgcca gagagatgtt
     1801 agccggcggt ggcaagatga tcctgggttc cgattcccat acccgttacg gtgcactggg
     1861 taccatggct atgggcgagg gtggtcccga gctggtaaaa cagctgttga accagaccta
     1921 tgatatcaac atgcccggcg tggtagccgt atacttaaag ggtgctcccg tgaagggtgt
     1981 aggtcctcag gacgttgcac ttgctatcat tggcgcagta ttcaagaacg gctatgtaaa
     2041 aaataaagta atggagttcg taggccccgg tgtggcttcc ctgagtgctg atttccgtat
     2101 cggcattgat gtaatgacca cagagaccac ctgtctgtcc tccatctgga agaccgatga
     2161 ccagatccgt gagttctatg agatccacgg acgtaccgaa gactataagg aactgaatcc
     2221 cggccaggta gcatactacg acggactgat cgaggtggat ctggacaaga tccgtcctat
     2281 gatcgccatg ccgttccatc ccagcaatac ttacaccatc gaagagctga atgcgaacct
     2341 gatggatatt ctggatgaga cggagaagcg tgcgaaagta agtctggacg gtgccgtaga
     2401 ctttactttg aagaataaag taaagaacgg caagttcatt gtagatcagg gtatcatcgc
     2461 aggctgtgcc ggcggcggat tcgagaatat ctgtgcggct gccgacatcc tgaagggcag
     2521 atacatcggt gcggatgagt ttactttaag cgtatatccc gcatccatgc ccgtatacat
     2581 ggagctgatc cgcaacggct gtgcggctac catcttagag accggtgcag tcatgaagac
     2641 tgcgttctgt ggtccctgct tcggtgccgg tgatacgcct gccaataacg cattcagtat
     2701 ccgtcactct accagaaatt tcccgaaccg tgaaggctcc aagttgcaga acggccagat
     2761 cgcttccgta gctctgatgg atgcccgttc catcgcagcc actgcagcca acaagggtgt
     2821 gctgactccc gcaacggact tcgatggaga tttcggaaaa tacaaatatc atttcgatag
     2881 caatatttac aagaaccgtg tcttcgattc ccacggtgtg gcagacgaga gcgtagagat
     2941 ccagttcggt cccaatatca aggactggcc cgctatgggt gccctgcctg agaatctggt
     3001 actgcaggta gtatccgaga tccacgatcc cgtaaccact accgatgagc tgattccctc
     3061 cggtgagacc tcttcctacc gctccaatcc tctgggactg gcagagttca ctttaagccg
     3121 taaggatccg cagtatgtag gacgtgccaa ggagatccag aaggcagaga aagagcgtat
     3181 gaacggcgga catccctgtc aggcagttcc ggtactgaag gatgttatgg gtgaagtaaa
     3241 gaaacagtat cccgatgcaa gtcgtgagaa caccggattc ggttccacta ttttcgcagt
     3301 gaagcccgga gacggttctg cccgtgagca ggctgcttcc tgccagaagg tattaggcgg
     3361 ctgggcaaat attgccaacg agtatgccac caagcgttat cgttccaacc tgatcaactg
     3421 gggcatgctt cctttcctga tccagcccgg agacctgcct ttcaagaatc tggattacct
     3481 gttcattcct ggtattaaga aagccgtgga agacaaggcc gaggaaatta aggcatatgt
     3541 ggtaacaacc ggagaaggta tgaaggaatt caccctgaag ctgggagagc tgaccgacga
     3601 agaacgtcag atcatcctga agggatgcct gatcaactat aaccgagtgt aactattcag
     3661 agacatgtga ctaaccatcg cagatgcgtt tcgcgaagag aggctgaata gttacaatga
     3721 gaagtaagga gaaaaaatat ggaatttaac aaaccggtat ccaaccccat gatggtggga
     3781 tccattgaat tattaaaagc agaggacacc ccggaacaca gacagatgtt cctggatgaa
     3841 ctgcagaaag caaagttctt gtctccggtc gtgatcgatc cggtgcctgt gcccgatgaa
     3901 aacggccggg tgaccatcgc aagagatgcc aaagtacagt tcccgatgct gtccaccgag
     3961 gacggtcgga agttcttcat ggcgttcact gactggacag agctaaaaag gtggagagat
     4021 gaggagaatc agcagacttt cgccatgaac tttgacgatt atgcgggaat gctgctgcgc
     4081 aaagatgcac agggcaatat cagcccggcg ctgggattcg tgatcaatcc cttcggtggc
     4141 aacatcgtgg tgaccagaga aatggtagcc agcatgatcg cagcaaagct gaaagcagcc
     4201 ggcagaccgg tgcctcccgc acccggaacc cctggtgcgc ccactcagcc caaacagcag
     4261 taaattaaca ctaaataaaa tgtccatagc ggacgatata agtagtagag aggtgacatg
     4321 gccagtcact tcctgctact tattttttac agtttgataa tgacaccacg gatggtaacc
     4381 agacagaggc atattatatg tattctgttt ctttaccatc ttttttagtt taacagttca
     4441 gccatgcaat atcatatgtt ctgcgtgggt gctggcacac aaagcagaac atatgatatt
     4501 gcatgagcgg agcgccgcat gtatgaaata aaagatgagc cgcttagcgg cgtcgactgc
     4561 ggagcaggcg cttttatgaa tacatgcggc ggtgaactgt taagcgttac aggaagcgcg
     4621 ccctgttggt tcgtggtgag gtcggggaaa ggagaaaatt acagatttga gaattgattc
     4681 cgcaagcata ggaatggatt ccgccagaag ctatcacgct tctaagacaa gcgtcagacg
     4741 atttgagata acggattatc aggcaggtct gacagatgta tccactaagg gacagaagac
     4801 accgaatggt gaggatacca cacagaacac ggaggaacag gagaacactg cagagaacac
     4861 cacgatgaca gccaaagact ggcagagtcg ttttctggta tcttctgcca gatgggatat
     4921 gcgaagcagt gccagtgaac agacattgtc taccatccgg gaatatacgg tgcgttatat
     4981 ttttgattta ttattcccgt ccaggaacag cactttgcgg gactggatgc aggaaaacgg
     5041 atggagcagc ggtaatcaga ccggaaacag ctggaataca gggaatagcg taaataatac
     5101 ccagacgaat tccggcagca cacagggaac cgcaacacct gcgtcagacg tgttcaatct
     5161 ggtcacgtcc cgtgtgaaaa tattgaacta tgtgagcgag acctggaaca tagaacagga
     5221 ggataccagt ttttccaccg tgggaagcgt gcggaccaag gatggcagag agatcaattt
     5281 taatgtaaat gtaaatatga gccgcagatg cgaggaatac tacaaagagc aactcaatgt
     5341 agcagaattc acgctccatg atccgctggt gatcaacctg gatactgatg tggcagaagt
     5401 gtcggatcag accttttatt ttgatctgga tgccgatggc aaggaagaag agatctccgt
     5461 gctgaatggc agcggctatc tggcactgga caagaacggt gacggcacta tcaacgacgg
     5521 cagtgagctg ttcggcaccc ggaatgggga tggctttgca gatctggcgc agtatgatga
     5581 ggatggcaac ggctggatcg atgagaacga ttccatctgg tcaaaattaa aaatctggtg
     5641 caaggatgaa aacggcaacg acgtactgta caaactttct gacaagggtg ttggtgctat
     5701 ctgtctgcaa aacgtttcta cggattttac cctgcaggga gaccgtaagg ctcaggacgg
     5761 cactaccaat gccaatgcga ccaatgctgt gatccgcaag accggtattt tcttatacga
     5821 aaacggaaat gtgggtacgg tacagcacgt ggatatggct gcctatgccg cacaggcata
     5881 gaataaatat aactattcag ttacttcgcc ctataaatag nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn tgccgcacag gcatagaata aatataacta ttcagttact
     6061 tcgccctata aatagcatca aatgttctta cgaatgcaaa cattcgacgc tatttacatg
     6121 gctctgaata gttccaaata aatgcaaatt tcatatcgaa gacattctga ataaaaccca
     6181 tgaatcgggc aagactatag gataaaccag ggaaagtggt tgaaacgatt gtatagcagg
     6241 cagttgactc agggaacagg aaggatgcgg ataggatatg agaagaaata aaaagagcgg
     6301 tattaacaaa gaacgtatta taatgttagc ctcttcagcc tttgtactgg cagcattgac
     6361 catgacaggg gtctatatga aggggcagaa tgtggagtcc agggatgatg gttatacctt
     6421 ggacttcact gcgcttccca acaatgcgga tgacaaatta caggagatca cggagaataa
     6481 cgacgaacat cttctgaagg aggacgagaa catccccgga ctgacggcaa ataatacagc
     6541 gggaaatgag atcgcgggag ctcctgtcac cgaggatgat ctggattata tgccccagga
     6601 ggtaggctcc ggcaacgtag agattccggg gctgacggat caggagacgc tgctggaggc
     6661 cgcacagaaa gagcagcaga attccgatgc cgaccgacaa aaggaagaga ccgggggaaa
     6721 gcagacagca ggcggggatg tggtcgttga gaagacactg cactttgcag aaagcgacgg
     6781 attacagcaa ccggtgcaga gtgatattct gatgcattac tctatggaca agagcactta
     6841 ttttgctaca ctggatcagt ataaatacaa tcccgccacc attttttccg cggaagaagg
     6901 tacggcagtg accgcctgtg cacagggaaa agtgacttca atcttcgaaa atgaggagat
     6961 cggcaaggcg gtgaccctgg atctgggtga cggctatgag gccacttatg gacagctgac
     7021 ggagatcaca gtgaaagaga attcctatgt gaatcccggc gacgccatcg gcagtgtggc
     7081 agcgcccacg aaatactaca gcaccgaagg aagcaacgta tactttgctc tgacccacaa
     7141 cggggaaccg gtgaacgggg aggcattgtt ccgctgatgt atattgtagg aggcaggatc
     7201 ctgaccatgg cgggcagaga gatccccgaa ggaatcctgc agataagaaa cgggaaaatt
     7261 gcacaggtag gtgccagagg tgaggtgaag ttacagccgg aagaaggaga gcaggtagtg
     7321 atcgcaaaaa atgcgctgat catgccgggc atcattgagg cacactgcca tatgggtatc
     7381 atggaagaaa aaaagagcac ggaaggggat gactgtaatg agacagtgga tcccctgaca
     7441 ccggctctgc gggctatcga tggtattaat cccatggatg cagcctttga cgatgcggtg
     7501 cgggccggaa ttactgcagc catgatcgga cccggaagct ctaatgtggt gggcggccag
     7561 tttgccatgg taaagactaa gggacgccgg atcgacgacc tgatcctgaa atcaccggcg
     7621 gccatgaagg tggcctttgg agagaacccc aaggtcaatt attccgggca gaacaaaagt
     7681 cctgtcaccc gtatggccat cgccgccatg ctgcgcaggg aactctggga gagcagagaa
     7741 tatctgcggc aaaagcagga agcggcggag aagggagagt attttgcacc ggattttgaa
     7801 aaagagtgct atcttccggt actgcgggga gacatcccgt taaaagccca tgtccaccgg
     7861 gtggacgata ttttcacagc aatccgcatt gcaaaagaat tcggtataaa aatgacgatg
     7921 gatcattgct cggaaggtca tctggtggcg gaagaactgg cgaaggaagg atttccggcc
     7981 attgtgggac cggatcttac cagccggaac aagatcgagg tgcagaacat gtcttttaag
     8041 accgccggag tgctgaaccg ggccggtgtg atggtcgcca tcaccacgga tcatccggtg
     8101 tcacagatcc agacactgcc gttgtgtgcc ggacttgcgg taaaagcggg acttccaatg
     8161 gaggaaggtt tccgggccat caccatttat cctgcgaaaa tctgtggcgt ggcagaccgc
     8221 attggcagcc tggaagtggg gaaagatgca gacattgcca ttttcgacgg aaatcctatg
     8281 gaaattttca cacgaaccct ttatactatt atcaacgggg agatcgtgta ctgtaatgtc
     8341 cccagggaat gaccggagga atattttgag atacagcagg aaaagtgcag ggatcctgtc
     8401 cctgctatta ctatttaaca gtataggtat gaccgcctgc gggcaggcaa cggcagagac
     8461 agggcatgta agcataagta tgaccggcat ggaaaacaca ccggtcatca actatacagt
     8521 gcctacactg acacccaatg tgctggtgga tcagcagggg tatgccgcgg tcggtgaaaa
     8581 acaggcagtg gtgaagggca gacagtcggt agagactttc cggctggtgg accgggagac
     8641 cggagagacg gtctatgaag gcacggtaaa gcagacagat tacaacgggg agctgtctct
     8701 gtacatcgga acagcggatt tcacggacta taccggggaa ggtgagttct atctggagtg
     8761 tgacaatgtg ggaagatccc tgacattttc tctgaaagaa gaccactatc aggaactgtt
     8821 ggaagcgttg tgcacggatg ttcatgacag atgtcaggat cgcagtatta ccgaggatga
     8881 gatcatcact ttgttggagg cctgtgagtg gtatccgcag gtgcttgcgg atgataatgg
     8941 caatgatatc ccggatctgc tggaatctat tgccgactgg ctggaaaaaa cggccaacga
     9001 cacagaaaag ccggaaccgg agaacatgtg ttacgtggcg gtgatggcaa aattcagtta
     9061 tctgtaccag aaatatgatg tgcaatatgc cacacaatgt ctgcagcatg cgtcgtcggt
     9121 gtacaccaag ctgatgtcga catccggcag agatgcggag aaattcatgg cgctgacgga
     9181 gctttaccgg gcggcgggac tatacactta ccgcaatcag atcctggagt ataaggactt
     9241 tttcgaggac aataccagtt atctggaaga aacggcctat ctgtatggct ccatgacgta
     9301 tctggccacc agacagccgg tggatgtgga cctgtgcact gtattcatgg aaagcatccg
     9361 gaatcggggc gaggagctgg ccaaacgttc ccataacatg atcgatgccg taatgaatgt
     9421 caacaatgga accgaggatc tgctgaagcg ggcagaagaa ttgtcctgtg ctaattatgt
     9481 gttatacagt tatcagtaca cggagatttt agaggatttt ctgcattacc tgatgggacg
     9541 gaaccgggat tcggtgagtt attatcccga agagggagac gctgcggatt atctgttact
     9601 gatagcccag caggtctccc tcgcggatag acattagaaa gatcagatca gttctttgtt
     9661 tttgaaataa gtttcggaga taatattctg tacataggtg cccatgctct tgcgagtctc
     9721 cttgtccagt tccttcggat aaataggctt accgtattca atgactacgg tggccttttt
     9781 tattttcgga aaatgatctt caaataccgc agcggaattc acaatagtca tggggataat
     9841 gggaacattt gctttctctg cgatcttgaa actgccctcg tggaagggaa ggaaggtatc
     9901 cggggtcttg ttgcgggtgc cctccgggaa gatgcagatg gaaatcccgt ttttggcttt
     9961 ctcaatggcc tgcagaatgg ttttcagtcc tgccttgatg tcctggcgat ccagaaagag
    10021 acagtgaaga tctttcatcc agatgttaag caggggccat tttaacattt cttttttcgc
    10081 aatataaccg gtaggtctgg gaacacggac gtaggtcagc accacatcga aaaagctgcg
    10141 atgattgccg acgtacagca cagcagtgtc ggtgggaatg ttctcttccc cgatggcaat
    10201 gactttcgtg ccggaaagac ggatgaccca gcggaaggcc cagtttacaa tggccaggga
    10261 actgcggtcc ttaagacagg ggttaaattt accgatgatc cattccacca gcattaacgg
    10321 cgtggacagg atcaggaata atactacaaa taatacgacg aatataaaac ggatcataat
    10381 aataccaatg cctttcagta acctgcaaac tttcaggtgc cttggcacct tgtgcaggca
    10441 caaatttgcg tgtgaaaaat taatttttca cactaaactc ctacggattc tttatttagc
    10501 aatgaaaagt acgcctaacg tgccgggacc acaatggctg gagactacac cgcctgctct
    10561 ggtttccaga atctcgtcaa agattcccag actttccaga taggaacgaa ccgcttccac
    10621 aatctcccgg tcgcagccgg agtgggtgat gaagactctg tcgggaacgg catttttcag
    10681 atcctcttcc atatcctttg tgtatgagag gatgacggaa cccagttttc cacggtattt
    10741 tttggaggca tccatggcac catccaccac gacgatcctg ggatgaagct ttaacatacc
    10801 accggccaga gccgctaccg cgctgcatct gccaccgcgg tgaaggtagg tcagggtatc
    10861 tacgacgaag ctggcccgga ctttgggacg gatggcttcg atggcagtag tgatctctgc
    10921 ggcagttttc ccttcagaag ccagaatggc agcctgtatt acctgcagac cgatgccggt
    10981 ggacaggttt gcggagttga tgatggtgac tttgtcggaa gcttccagtt cttctgcagc
    11041 aagccgcata acgttgccgg aggtggacat gctgtcggag atggagaaac agatgatctc
    11101 ccggccttct tccacaaaag gctgcatgac ttccatggcc tcggtcagag aaggagcaga
    11161 ggtcttgggc gtggtcttgt tttcatcgga ccaccggaaa atttcttccg gcgtaatatc
    11221 cacgccatcg cggtattcat cctcgcccag cagaatgtgc agaggcagga tggtaatgtc
    11281 atacttttcc actaattctt ttgacagatc acaggtactg tcagagatga tcttaactgt
    11341 ctgtgccata gaatctacct tcctttttat tagtaattat tcccctttgg cgatctgcct
    11401 tgcgacatag acaccgctgg cggatgcatg ggacagggaa tgagtcacac cgctgccgtc
    11461 accgatgatg tacaggccct tgtgacagct ttccagatgt tcgtccagtt ccacttccat
    11521 attatagaat ttgacttcca caccgtagag cagggtatcg tcattggcgg taccgggtgc
    11581 gatcttgtcc agtgcgtaga tcatttcgat gataccgtct aagatacgct tgggcagtac
    11641 caggcttaaa tcaccgggag ttgcggacag ggtaggctct accatgccct cggcaatacg
    11701 cttcgcatta ctgcggcgtc ctctcaccag atcgccgaaa cgctgtacga taacaccgcc
    11761 gcccagcatg ttggagagac gggcgatact ttcaccgtag ccgttgctgt ccttgaaggg
    11821 ctcggagaaa tgcttcgcta ccagcagggc aaagttggtg ttctctgtct gcttgtcggc
    11881 tccttcgtag ctgtggccgt ttacggtgac gatgccgttg gtgttctcat ttacgacgat
    11941 accgtgggga ttcatacaga aggtacgcac ggaatcctcg aatttttcag tgcggtatac
    12001 gatcttgctc tcatagagtt catcggtcag atgagagaag atgactgcgg gcagttccac
    12061 ccggacgccg atatccacgc ggttggactt ggtgttgatg tccagatctt tacagatctg
    12121 ctccatccat ttgctgccgc tgcggcctac ggaaataatg cacttgtcgc agtcgtaaga
    12181 agcgttttcg cagcagatac ggtaaccgtc accaacggtc ttcaccgttt tcaccggggt
    12241 atcgaaatag aaagtcactt tatccttcag ttctgcgtac agattttcca gcacgatgaa
    12301 gttgatatct gtacccaggt gacgtacgga agcgtctaac aggttcagct tgttctgcat
    12361 gcagatcttt ttgaaagagg tgccagcagt ggaatacagc ttcgtgcctt caccgccgaa
    12421 cttcatgttg atgtcatcca catatttcat caggtcgatg gcctgactct tgccgatata
    12481 ctcgtagaga gtgccaccga aagcgttggt gatattgtat tttccatcgg aaaaagcacc
    12541 agcaccaccg aaaccgctca tgatggaaca gctcttacag ttgatgcaac tcttgatctt
    12601 gtcaccgtcg ataggacagt ggcgcttctc cagagggtga ccggattcga aaacggcaat
    12661 tttcaggtcg ggattctgtt gcaccagttc ataagcggag aagataccgc cggggccggc
    12721 accgataatg attacgtcat atttcatagg gataagtttc ctttctcttt gctctcttgt
    12781 aaggatttgc gggatgtata ctcattttga acccccaaaa acattcaaaa tatagtatac
    12841 catattttta gataaaaagg ggaatagaaa tatagcaaaa tatgtgataa aatcataagt
    12901 tatagcggtt cttctgcaga tgcggatgaa cggtgcggga agataacggg attaggaaga
    12961 gaattacagg aaatgaaatt atggaagcaa cacagaagaa aaacaaatat gaaatagata
    13021 tgtgcaatgg cacgattatg gacaagctga tctcctttgc actgcccctg atggtgtcca
    13081 gcattttgca gctgctgttc aatgcagtgg atatcattgt ggtgggacgg ttcaccggaa
    13141 gtcaggcgct ggcggctgta ggttccacca cagcattgat caatatgttc gtcaatctgt
    13201 ttatcggcgt ttctctggga gccaatgtat tgtccgccag attctatgct gccgggaaag
    13261 agcgggagat gagcgagaca gtacatacag ctatgacctt tgcattgatc agcggtattg
    13321 tcatggtatt cgtagggctg ttctgcaccc ggggagcgtt ggaactgatg gataccccgg
    13381 cggatgttat tgatcaggcg gcattatata tgaaaattta tttctgcagc atgccctttt
    13441 tcatgctgta caattacgga gctgcagttc tgcgggcagt gggagacacg aagcgtccgc
    13501 tgttgttcct gatcatatcc ggagccatca atgcggtatt gaacctgttt ctggtcattg
    13561 ctttttccat gggtgtggcg ggggtagcca ttgctacagt gatctctcag gtgatctccg
    13621 gtgtgctggt attatggtgc ctgtttacta cggacagctg ttaccggctg cagatttcaa
    13681 agctgggaat aaaaggggag tatctgctgc agatctttca ggtgggggtt ccggcgggga
    13741 tccagagcgt tgtcatcaat ttctccaatg ttctgctgca gtcctcggtg aattccttcg
    13801 gctccatcgc catggcaggc tatacggcgg ccaataatat ctttggattc ctgttcgtgt
    13861 cggtgaattc cattacccag gcgtgtatga gcttcaccag tcagaactac ggtgccggga
    13921 agaaaaaacg tatggacagg gtactgatcg attgtatgat cctgtcggtg atcgtagcgg
    13981 taacgctggg tggaagcgcc aatctcttcg gtccgcagct gcttcacatt tacacaacgg
    14041 atgcggacgt tattgcctgt ggaacggaga ttctgctgta tacgacgttg acttactttt
    14101 tatgcggcat catggatctg attccgggag ccttgcgggg catgggacat tcggcggtac
    14161 ctatgatatt gtccattatc ggtacggtgg gaacgagaat cttctggatc tacgaagtat
    14221 tcccaacgca ccggtcgttg atggtactgt ttttatctta tccggcctcc tggctggcaa
    14281 cgatcctgct gcaggcggta tgtttctatt ttgtcagaaa gaaagtacac agcaccatgc
    14341 cgcaggaaga accgttataa gcggtcggtt ttcgggagtg aaagtcccgg gatgatcgag
    14401 cgtatgtagg gattatttag aaaattgagg gaaattatgt tagcaaaatt taagaaaaaa
    14461 tatattggag actgggattt ttaccagaag tatcttttac tggctattcc catgatcctg
    14521 cagaatgcca ttaccaatct ggtaagcttt ctggataaca tcatggtggg acagctggga
    14581 acggagcaga tgtccggtgt tgccattgtg aatcagctga tctttgtata caatctggca
    14641 atcttcggag ctgtgtcagc tgccagcatc ttcggtgccc agtatttcgg aaagggcaat
    14701 cataagggac atatgtattc ctttcggttt aagctgtatg ccacgctgtt ggtgacaggc
    14761 ggagcgatcc ttctgttcct gacgaaaggt tccgcactga tctccctgta tctgacggat
    14821 acggtgggga acggggccac ggacttggca ctgcagtacg gactggaata tctggccatc
    14881 atgatggtgg gactgatccc ttttgcggtg aaccagtcct acgcgacaaa tataaaagag
    14941 accgggcaga cctttatccc gatgctggcc agctttgtgg cggtgggtac taatgcgatt
    15001 ctggactatc tgatgatctt cggcatcggt cctttcccgg agctgggagt gcagggggcg
    15061 gcgctggcta ccgttattgc ccgttacatt gaggcactga tcgtggtgat ctgggcacac
    15121 atccatcggg gaaaaaaccg ctatctggag ggagcttaca ccggctttgg tattccgctt
    15181 gcggaattga aggcgatcct gatcaaggga tttccgctga tgatgaacga ggtgctgtgg
    15241 gcagcgggca tgacaacggt gacccagtgc tattctgtca gaggtctgga cgtggtggcg
    15301 ggactgaaca ttgcctccac catcacgaat ctgttcaata ttgtctatct tcagctgggt
    15361 tcctgtatca gtattgtggt gggacagtat ctgggtgcag gtaaattgga ggatgccaag
    15421 gatgcagaca acaagatgat cgtattcagc gtcttctgct gtgtgatcat ggcggcattg
    15481 atgttcgtgg taggaggatt tttcccgggt atttacaata ccacggagga gatcagggaa
    15541 ctggctacca gttttattgc ggtatcggcc atcatcatgc ccttctgcgc cttcagccat
    15601 gcgtcgtatt ttaccctgcg ttccggtggt aagacggtgg tgactttcct gtttgattcc
    15661 gtatttacct gggtgatcgt ggtgccggcg gcatatctgc tggcgcattt caccggcctg
    15721 gggatcgtga gcatctattt tctggtgcag ggtacggaac tgatcaaggt gatcattggt
    15781 tacctcatgg tgaagagtaa tgtctgggtg gtccagatgg tataaattac tatttgtgcc
    15841 gccagccgaa ggctgctgga tggagattgg catgaaaaat aggagaaggg cgggaaactg
    15901 acatgaaaat gaacatggct cagaaagttt tactgatcat aggaggcgca tttgcgggga
    15961 tcggcgcagt gctgacaatg atattcggaa gcatcggcat ggtgttccgg cccatgaggg
    16021 catttctggc attgccgctg tttttcctga tactgggcat ttgttttatc gcagcagtat
    16081 tattcgggca gcataaaaag tcactcatcg taaaaaacgg aatcagatat gccgccaaga
    16141 tctacggtta cgtggagaat acggcgtata tggtaaacgg cagattcccg gtgaatgtga
    16201 tcgtgcatta ttttgacaag aatcagatcg agcgggaggc agtgattccc actgcgtttg
    16261 aaaagggcgc ttcgacctat cccatcggta tgaccatgga tatctacgaa tatcagggaa
    16321 aatacggttg ggatccggat tccgtccggg atgagattct gccgggagag caggaactga
    16381 tggacgataa gcccgtggat ccttccaagc ttcgcatgac cgccgtgcag tgtccgaact
    16441 gcggtgcttc ctatcaggcg gcagcaggat ataccggacg ctgcccgtat tgcggaagct
    16501 atcataatgt agaatagcag aatagttgag ctggggtgga tcagtggatc gtttcctgca
    16561 aataccgcag catcgtctgt gacaccacgg agttggcggt cttggagaaa gccagtccca
    16621 gcgtacggtg acaactggga gataaggggc tggagaccac accccgtgcg aaagctttta
    16681 atacaagttc ctgagagatg gtgacaccga gaccgttccg gacgaaggac atgatcaggg
    16741 tatcgtcccg gaagctgtaa gagatctttg ggcggacagg gcatttggtc agcagatgct
    16801 ggatgtcgtt gtcacagccg ggggtctcga tgatcagagg atattcgaac agttccggca
    16861 gggtgacgct gtttttggca gccagcggat gtccctcggg gaaaacagcg cacagcggat
    16921 cattcaacag tggataaaag gtcagatcat ccgccacgat ggaggataaa aatccacaat
    16981 ccacctgccc gtgaatgatc cagtcccgga tctcatcata attgccgtca aaaatttcta
    17041 catccacttt cggataattt tcccggaaaa aatgaaccac atccggcatc cacagagcgg
    17101 tgatgctgga aaaactaccg acccgcagtt tcccctcaat cttattgttg atattgaagg
    17161 cggcctggcg cagcttgtct ttctggttga ccatttcctg aatgtaaggg atcaggcggg
    17221 caccgttgtc ggtgaggctg acaccccgtt taacccgggc aaaaagctgc acacccagtt
    17281 cttcctccag tcccgccatc atctggctga tgccggactg ggtataattc agtgcttccg
    17341 ccgccttgga gaagcttccc gtttcacata cttttaaaaa tatctcgtaa cggtcttttt
    17401 gttctttcat gatcttcacc ccgtaaattt atgaatttca aattcatcgt tcaccaatta
    17461 tgcgagcaga ttggctttct tgtacttatt atagtataag ctcacgaatt cgtgcttcgc
    17521 actttacatc ctgcttcgca ggatattcgt gagctgatga gtccagaaaa acaaatgtct
    17581 gctttgcagc cacttgtttt ctcttgagac tcatcacatt acaaatactt atgcatacta
    17641 gtaaaaacat tcgttttact gatagtatag catatgctat cctcatgtca caag
//
LOCUS       DAWEJW010000059        17211 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1255_length_17211_cov_57.068547, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000059 DAWEJW010000000
VERSION     DAWEJW010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 17211)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17211)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17211
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1255_length_17211_cov_57.068547"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2633..2732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13869..13968
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15679..15778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4760 a         3655 c         4729 g         3767 t
ORIGIN      
        1 tgcggcatgt tcttcattcc gctgggaatg atatttatct tccgaaatgc gctgcagggc
       61 tgtggcttcg gcttcatgcc catgatgggc ggtgtggtgg aattgttgag ccgtggtatc
      121 gtggcttttg cggcagcgca tctgatgagt tatgtgggtg tgtgctttgc caatgcctca
      181 gcctggctga ccgcaggtat tttcctctgg atcgcttatc atttcctcat gaagaagatg
      241 gtgagggaca aaaagggtca tgaggaacgg atgctggcag aagcaatgca gtaatgcagt
      301 aaaacggctc agggcaggat cttttcaaaa aacagtcctg ccaggaacca ccagggagca
      361 tagtccagcc ggatgatccg gtggatgtgg aaacggcttt taccgtaatc ccaggggcag
      421 agaccatgct tccacaatag gcgcccactg agatattcca cggtgaagat catagccata
      481 tagaacagcc cgcgcccggg acgactgata ttcaggtgct caaaaagccg tttgaagggt
      541 gcaagcaggc aggcggagcc gtagataggg aacatccaca gggaagtatt accggtaaga
      601 cgcagatccc tgcgccgaag ggagtgcagc gctgtgaaca ggatctccat gcaccagccg
      661 cacagcccgc acaggagaaa gctcctgccg aaggttttaa aagagtttgc gatggatttt
      721 gtcattttca tggacatagt atatgaaatt ttcagtgtat gatacgggag agactgctat
      781 gaactatcag gaagcaatga aatatatgga aaaagtggga acctacggca ttgttccggg
      841 gctggacagc atcagagagc tgtgcagaag gctgggagat ccgcagaacg acctgaaatt
      901 cgtacatatt gcgggaacca acggaaaagg ttccacactt gcctatattt ccaatatctt
      961 acaggctgcc ggataccgtg tgggaaaata tatatcacct gttattattg aatattgtga
     1021 aaaaatccag atcggaaagc agaagatcac gcataaggat ctgtgtgaag gactggagat
     1081 cataaaaaag cattgtgatg ctatggtaag tgacggcttc catcatccga cgccttttga
     1141 gattgagacg gcgctggctt tttggtattt ccgggaaaaa cagtgtgata tcgtcgtact
     1201 ggagaccggt atgggcggca gggaggatgc tacaaatctg atcacgacca cgcaggtggc
     1261 ggtactggct tccatcggta tggatcatat gaaattctta ggtaacagtc tggaggagat
     1321 tgcagcacac aagaccggaa tctgtaagcc gggatgtcag gtggtctcca tgcggcaaaa
     1381 agaagcggca cagaaggttg tggagcagac ggcggcagag cttggctgta tactgaccat
     1441 cgcagatgtg gcaaatgcaa agcatgtgaa atacggactg aaaaagcaga ccttcgatta
     1501 tggaaattat aaaaagctgg agatcaccct tgcggggaaa ttccaggtag caaatgctgt
     1561 gctggcggta gaagcggtgc aggctctggg gaaatgcggt tatgagatca aagagactgc
     1621 cattcggaaa ggcctgaagg agactgtctg gaacggaagg ctgcaaatac tggaagaaca
     1681 cccgttattt atagtggacg gtgcacataa cgaggatgca gccgcaaaac ttgcggattc
     1741 catagaattc tattttacaa ataaaagaat tatctatata atgggaatgt taaaggataa
     1801 ggaagtggac cgcgtgatcg cactgacgga gaagtatgcg gatcagattc tcacggtgac
     1861 acctccggac aatcccagag ccatgcatgc ttatgatctg gcaacggagg tggcaaaggt
     1921 acaccccaat gtgacggcgg tggacagcct ggaagaggca gtggagctta gtcacctgct
     1981 ggcaggacgg gatgatgtga tattgtgctt cggttctctc tcttatctgg gaagaatcct
     2041 gaagctcatg gagaagagac agacagcagg gaataaacgg aaaggaagcg gtgaaaatgg
     2101 tagatcagaa gaaagtacag gaagcaataa agcttttact ggaaggaatc ggggaggata
     2161 ccgatagaga gggactgaag gagacaccgg agcggatcgg caggatgtat gaagaaatct
     2221 gcgatggtat ggatcaggat gcgggagaac atctgtcgaa ggtcttctcc gtagacaata
     2281 acgagatggt actggagaag gatattacct tctattctat gtgtgagcat catctgatgc
     2341 ctttttacgg caaagctcat gttgcgtata tccccgatgg caaggttgtg ggtctgagta
     2401 agcttgccag gactgtggag gtatatgcca gaagattaca gatccaggag cgcatgacgg
     2461 cacagatcgc cgatgatatc atgaaatatc tggcgcctca gggagttatg gtaatgttgg
     2521 aagcggagca tatgtgcatg accatgcgcg ggatcaaaaa gcccggcagt cagaccgtta
     2581 ccatggtgac aaggggagtc tttgcggaga ataaggaatt gcaggacaga ttnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttaccatg gtgacaaggg gagtctttgc
     2761 ggagaataag gaattgcagg acagattttt acagctggtg aaccggtaga gattacggat
     2821 gcggaagaaa gctgctaagc gactgggatg aaatgatgga gactccgatg gatagaattg
     2881 ataaaatatt aaatcatgat ttgtttttat accatttagg acgaaacaat gcggcagaag
     2941 cagacagacg cttctgccgt catggtatgg tgcattttct ggatgtagcc aggatcggta
     3001 cgatcatagc gctggaggaa gggcttaaga tcaaccggga atggatctat gcggcggcac
     3061 tgctccacga ttgtgggaaa catgaacagt acgagaatgg cattccgcat gagcaggcca
     3121 gtgcacggat cgcaccggag attctgaagg cgtgtggatt caacgatgaa gaaacgggtg
     3181 ttattgtaac ggcaatctcc agacaccggg atcctgaggc ggcgaaggag aagaacctaa
     3241 atggcatcct gtatcgggcg gataaggcaa gccgtgcctg ctttgcgtgc gatgcggaga
     3301 aggactgtaa ctggaaagac ggcaagaaga acctgacgat caggtactga gtgcaacagt
     3361 ctgaaccgag aaattcgcaa agctgtaccc ggtaggggaa catatccggg caaaaaaaaa
     3421 aatacaaaca tgagggagaa aacaaatgaa gattggcaac agagagtttc agaccaaagg
     3481 acatacttat gtaatgggga tcctgaatgt gaccccggat tctttttccg acggaggaaa
     3541 gtggaatgac agagaccggg cattgaagca tgtggaagaa atgatcgctg aggggatgga
     3601 tattgtggac atcggcggag agtccacgcg ttcgggttat accctgctat ctgacgagga
     3661 ggagattgcc agagtcgtac ccatgatcga ggcggtgaag gcaaattttg acattcccat
     3721 ttccctggac acctacaaaa gcggtgtggc agaggcggga atccgcgcgg gagcggacct
     3781 catcaacgac atctggggat tgaaatacga cagacacatg gcggaagtca ttgcaaagag
     3841 cggtctgccc tgctgtctga tgcataaccg taaggaagcc gattatcggg atttcatgca
     3901 ggatgtggta gcggatctgg cggacacgat ccatttggcg gaagcggcag gcatcgcgga
     3961 tgagaagatc atcttagacc ccggtgtggg gttcggcaag acctacgaga acaatctgga
     4021 gatcatcaac tgtctggaag aactgaatat gttcggctat cccctgctgt tgggatgcag
     4081 ccgcaagtcg gtcatcgggc tgacactgga tctgccggtg acggagagag tggagggaac
     4141 gctggtgact accatgttcg gcgtccagaa gggatgtatg tttgtccggg tgcatgatgt
     4201 gaaagaaaat gtccgcacca ttaaaatgtg tgaagctatt ttaaacagtg gaaaataaaa
     4261 agctctgaaa aggtatcggg aaaggcggag tcagcaaatg agaaatgaga atcggaaaga
     4321 tcagatccgt atcgaagaac tggaagtata tgcgcatcac ggcgtatatc cggaagaaaa
     4381 tgaaaaagga cagcattttt acgtgaatgc caccctgtat acaaacaccc gcccggcggg
     4441 aatggcggat gacttaagtc tctccaccaa ttacggagag gtctgtcagt ttatcacgga
     4501 gttcatgcag cagcatactt ttcaattgat cgaaacggtg gcggagcgga ctgcttacga
     4561 agtattacag cagtttccac tggtacaggg actggatctg gagatccgca agcctgaggc
     4621 tccgattccg ttaccctttg gcagtgtatc tgtggcaatt cacagggaat ggcatgaagc
     4681 ctatattgcc gtgggttcca atatggggga cagcagagaa catatcgcgc gggcactggg
     4741 ccagcttaag aatagtaaag atatcagagt agaaaaggta tcggagcttt tggagacatt
     4801 gccatatggc ggtgtggaac agaataactt cgtaaacgga atgtttgaga tccggacgct
     4861 gcttgcaccg gaagaactat tacaggagct gcaccgtatt gagcagatgg aaggaagaga
     4921 acgcaagatc cactggggcc cccggacact ggatctggat attattttct atgatgactt
     4981 gatctacagc tccgaggatc tggtgatccc gcatgtagat atggagaacc gttattttgt
     5041 actgaagcca ttaagtgagc tggcaccgaa tttccgtcat cccattactc acaagaccgt
     5101 agcacagatg ctggcggagc ttcccgtgga tctcacggaa ggaagcgaag cgtaaaacgg
     5161 gcacagagac cgatgaacag accggtcacc agaccgctta acagtaaaaa cggaagatag
     5221 accagcacag cgggaacgga agtcagtaaa aaagcaacac caatctgccc cagattgtga
     5281 aaaacagcac ctgtcatgct tgcgaggaac aggtaattcc catgcaacag cagcttcaca
     5341 agcaacatgg caaacaggca gagtatacct ccggacaggc tgtacaggag actcatggcc
     5401 tgcccgaaga acatggtcgc taaaaggatc cgcaccagaa gcacgaaaaa ggcgtctttt
     5461 gcggaatatt tccatagaac aacaagcgtt attatattgg aaagccccag cttgatgccc
     5521 gggatcggga caatgggggg cagagcactt tccacggcat acagcgccag tgcaatggcc
     5581 gccagcagag aaagcgtcgt cagttttctg gtagttacag gtggtttcat aaaaatctcc
     5641 gtgttttcgt gattcgtaaa tggtccggtg gtttggtacc gtgcattttg gtacgctgtg
     5701 caggcaaaaa tggtattctg tcaataggtc acgccgtccg gaaccggtgc agtgtcatcc
     5761 ggaacggaaa cctcttctct gacccggatc accagccgat tgggcagaca ggtgatgggg
     5821 agcagggagg tgctgataaa tccctgatgc acgcagatct gatccggaca ggacgcagag
     5881 acgatggcaa tgctgcccgg acggacacag acggtattcg tggcaccggc attcccggag
     5941 acctcaaagg tatattcggc ggtcaccgta tccagacgga tggtctgcaa taattcgccg
     6001 ttctgataga gatcggcata gagagcctga ccgttttcta cggaactgtg cgagactaca
     6061 tagatccgca ctacgcaaag gacagctatt acaagcagag cggtggtaag gagaactgct
     6121 ttttttcgat cattcatgga aagattcata ggaataagga actcctttct gatgagaaca
     6181 gtatagcaaa aggaatgaaa aaaatgaaga agtatttgaa aacgccccgg gtgttgcggc
     6241 gggcaacgtt actggcaatg ctcccggtgt tttttctagc gggatgcggg cagaaaacgg
     6301 aatgcgaaaa aagcatagat acagccatgg gaaccgtgat ctcccagacc gtttatgtta
     6361 ccggaaattc cgcaaccaca acaaatagtg aaataaatga gaaaataaca gatgttcttt
     6421 tacaaaaact aaacgatctg gaacaaaaag aactttcctg gagactggaa agtgcggaag
     6481 tggcgcggat caatgcagcc gctggagaag ggcagacctc cgtatctcct gcgatggctg
     6541 agtggctggg aagatgccgg cagatctcag aggagagtgc cggggctttt gatgtcagta
     6601 tcggccggct cagtcgtctg tgggacattg atacctgggc ggctgccgga gattccgggg
     6661 attatgaact gccgaggcag gaagagatcg cggaggctct tgccaccgca ggctggcaga
     6721 aaattcagtt ggaacaacag gcggacgggg attcggtgag cattcccgca gaaatgcagt
     6781 tggacctggg agctgtggga aaaggtgtgg cgctggatga gatccttaag atactggaag
     6841 cgcatccgga ggtaagtggt gccgtgattt ccgtgggcgg cagcatcctg acttacggaa
     6901 gcaagccgga gggcggagcc tggcagatcg cagtgacaga tccgctggat ccttcggaaa
     6961 gtgtgggcgt gctgacactg gacggagggc actgtgtatc cacatcggga gattatgaac
     7021 gctatgtgga agtggatggt gtcagatatc atcacatact ggatccacgg accggatcac
     7081 cggcccacag tgatgtggcg ggggtcacca tcgtgacgaa ggacggattc ttaagtgatg
     7141 ccctgtctac ggcctgcttt gtactgggac aggaggaagg ccagaaactt ctggaaaagt
     7201 atgatgcgga aggattgttc atcgaccatg agggaacgat caccatgaca gagggcatgc
     7261 ggcaatattt tcatttgtcg aattcgcaga aatagtgtat agtaaatata gttaaccgta
     7321 gataacagtc agtgcagaaa acctctgttg agagagggat cacagagtaa ataatggaga
     7381 aataaaatgg aagagaagaa cagttatctt tccgcagatc cggaaaccat ccggcagatc
     7441 gatgagaaga tgccgccgga ggaggagtta caggatctgg cagaattttt taaagtattt
     7501 ggagatgcta caagactgaa gatcttgtcg gtactgctgt gctccgaaat gtgtgtgtat
     7561 gacatagcca ctctgcttgg tatgtcacag tctgccattt cccatcagtt acgggtattg
     7621 aagcagatgg atctggtgaa gaaccgcaga gacggcaaga cgatctttta tgcactggcg
     7681 gatgaccata tcatcacgat tctgaatcag ggcctgaacc atatcgagga aggttaaaat
     7741 caatttgcaa cgggaacgaa aaataagagc cgcaggcgga atgtctgcgg ctcttgctat
     7801 gctattattc tgtaactatt cagagcagta ttcgcaaaat cgcatcttcg atgctttctt
     7861 tttgctcata caagctgctt cgccaaagaa atatgagcgg atgctcttac aaatgcaagc
     7921 attcgacgaa catccgctca tatttcatgg ctctgaatcg ttacattatt ttttcaaagc
     7981 atccagcgct gcctggctgg ctgcatcaaa tccgctgtag ctctgactgc ccaaagggga
     8041 gtgataccag gaagagtcat ctccgaacat ctggaaatag acatactggg aagtcatatt
     8101 gatggcggta tagttgcctt cggcaatgac ccaggaatca cttccgtcat cccgcatgca
     8161 tttcaggcag gcttcctcgc cgttcacttg atagtacaca taatcatatc ccacattaaa
     8221 gcagtcgatt ctttcattgg tcaggacttc aatctcattt cgggacaggg agtatcgaca
     8281 cagtctgtaa ttgttttcca catccatata gtagacataa tctccctgta cgatgggata
     8341 ccacagattg ccgtcccata cggtggaaac tccgtccgtt gcggtattca tggcatagag
     8401 atagtggttg tcctgtgtac cgttgtaata aatggtgccg tttacggcgc tggcgggatt
     8461 gatctcgaag ttggcaagtt ccgtcttgtc agacttgtcg atcttcatac ggtaaaataa
     8521 aggcccgtta tcatccacgg tcataatata gaggttgctt cccaccagct gggcggtgga
     8581 aatgggatct ctggtaagtc ctgtgacgtc gctgccgttt agcttacagc ggttgaagga
     8641 acgcacagtt cgcagcgcac cgatcccggc atccccggaa gctccggtct gataatagta
     8701 gaggaattta ccgccagcca ggatattttg tacggaggca tttccaagtt ttttgatatt
     8761 ctgctccgaa ggatccatgg agtacagggt gtcgctgtca tagacattgg caaaatacac
     8821 ttttccatca tactcgcaga agtagccgga attgttcaga tttccggcgg tgttccccac
     8881 ggtctccggg ggattgtcag ggatccggtc tcccacgatg gcaaatgcac cgatgatgat
     8941 cacgaacaga agaaaaggaa tcagaatcaa taaggctttt ttatctttca taagcaatcc
     9001 ttctttatgt aaaatgaaat gtaaagtttt tgtaatatct aatattatac cttggttctt
     9061 gcttgctaac aagggaaaag tgagataaaa tgtaactatt cagagccaaa gggcgaagta
     9121 accactatga gcaaataaca tgcaagcatg ttattcagaa aagcctcgca gaggcggttt
     9181 tgcgaatgtg gttctgaata gttacatttg gagtcagcgg attccgaatg tttgagaatt
     9241 gcgggagctg ctttgcagcg gagcaattcg tggatatcag ttaagttaag gagaacagca
     9301 tggatttatc acagttgaga caacagatcg acaccattga ccggcagatc gtggatctct
     9361 atgagaagcg catggatgtc agcagacagg tggcggaata taaaatagag accggcaaaa
     9421 aagtatttga caaacagaga gagcaggaga agatcgccgg agtcaaggca ctgacccata
     9481 atgatttcaa cagccacggc gtggaggaac tgtttgaaca gatcatgtcc atgagcagaa
     9541 agctccagta ccagctgctg gcagcccatg gcagtgaagg cagactgccc ttcatcggtg
     9601 tggacgaact ggagaccgag tgcgccagag tggtctatca gggggcggaa ggctcctatt
     9661 cccaggcagc catgcgtaaa tttttcgggg agaatgtcaa tgcgttccat gtggagtcct
     9721 tccgggatgc catgagcgcc atcgatgaag gcagtgcgga ttttgccgta cttcccattg
     9781 aaaattccac ggcgggcatt gtcaatgaaa tctacgatct gctggtggaa ttcgaaaact
     9841 acatcgtggg agagcaggtg atccccatcg aacactgcct gctggcactg cccggcacga
     9901 agatggaaga gatcaaaaga gtctattccc atccccagtc cctgatgcag agcgccagat
     9961 acctctccga gcatgactgg cagcagatca gcatgcagaa caacgctttc gcagcactga
    10021 aggtggcaaa ggacaaggat cagacccagg cggccatcgc cagcgaatac gcaggagaga
    10081 cctacggact ggaaattctg gaaaaaggga tcaacgattc cgacagcaac tccacccgtt
    10141 tcatcattgt gacgaaccag aagatcttcc gcaaaaatgc caacaagatc agcctgtgtc
    10201 tggaaatccc ccatgagagc ggatccctgt accacattct gtcccatttt atctacaaca
    10261 atctgaacat gaccaagatc gagagccgcc cgatccccga gaggaactgg gagtaccgct
    10321 tctttattga tttcgaaggc aatcttgcgg acagcgcggt gaaaaacgca ctgcggggac
    10381 tcagagaaga agcccgcagc atgaagatac tgggcaatta ttaagcataa caactattca
    10441 gaaacccatt cgtaaaaccg cctctgcgag gcttacttaa ataacatgcc tgcatgttat
    10501 ttgctcatgt ggttacttcg cccattggct ctgaatagtt aaaacatata aaacaagaag
    10561 gttagacata tgagagaaaa agaactgatc gtataccgtg atttcgagga cggagaactg
    10621 ctgtatgaca tggcgttcct catgtcccat tacgatgacg aatattacaa cacggaagat
    10681 atggcggcac tgttttacga gtgcattcat gatttgatcg atctggcagg caattacggt
    10741 ttccacggga atctgtggca ctgctatctg gccaatctcc tggtgaataa cgagaacagc
    10801 tacagctgtg gctgcgagat ccgcggagag atcgtcggtt ccatcaacga tgcggcactg
    10861 catgacatcc gcattttcaa ggaattctat gattttgatt ttgcacccat gatggagcag
    10921 cttcatgtgc cggaattttc catcattgaa aattatgcca gcagcatgca ggagagcaag
    10981 gtgtacaata agagaatttg tgcccgcatc tgtgaactgg cggaaaagtt ctgcgcagac
    11041 ggaacggccg aggagatgaa ggctacactg acccagttct ataaggaata tggtgtcggc
    11101 aaattcggcc tgcacaagtc cttccgcatt acccatgacg aggagggtgt acatatcgtt
    11161 ccgattttga acattgccca tgtgaaactg gatgatctgg taggttacga gcttcccaag
    11221 aaaaaactgg tagacaatac cgaggctttt gtaaacggta aaaaggccaa caactgcctg
    11281 ctgttcggtg atgcgggaac cgggaaatct tccagcatca aagccattgc caatgaatat
    11341 tatgacaggg gcctgcgtat tatcgaggtc tacaagcatc agttccagga tctgaacacg
    11401 gtcatcagcc agatcaagaa ccgcaattac aaatttatca tttatatgga tgacctgagc
    11461 ttcgaagagt tcgagatcga atacaaatac ttaaaggctg tcatcgaggg tggtctggag
    11521 aaaaagccgg agaacgtcct gatctatgct acttccaacc gccgccatct catccgcgag
    11581 aactacagtg acaaggagga gaccagagag gatatgcaca ccagcgatac cgtacaggag
    11641 aagctgtcgc tggtataccg tttcggcgtg accatttatt tcggtgctcc caacaaaaag
    11701 gaattccaga atatcgtgaa gactctggcg gagcgctatc atgtggagat gccggaagag
    11761 gaacttttac tggaagccaa caaatgggaa ctgtcccacg gcggactgtc cggaagaagt
    11821 gcccagcagt tcattgatta cctgttagga aagtgaggaa ccttatgagt gtaaaaacct
    11881 tcgcagccat tgatgtgggc agctttgaac tcaccatgaa aatatttgat ctctccggga
    11941 aaaataccat gcgggagatc gactgtatcc gccagcggat cgacctgggc tcggacacct
    12001 atgcccacgg gaagattagc aatgagaaaa tagatgacct gtgtcatact ttgaaagaat
    12061 ttgcacagat catggaatcc tacaaggtga cagcttacaa agcctacggt accagtgcca
    12121 tccgggagac ggaaaatacc ctgatcctac aggatcagat tgaacagcgt accggcatct
    12181 gggtggagac cttaagcaac tccgaacagc gtttcctgga ttacaagtcc attgcctcca
    12241 agggagagac tttccgcaga atcattgaag aaaagacggc gatcctggac atcggcgggg
    12301 gtagtatcca gatttccctg ttcgacaagg atacactggt ctccacgcag aacctgcggc
    12361 tgggggtact gcggctgcag gaactgctga atcatctgaa cgcgggcagc actcagatgg
    12421 agcgtctggt ggacgaactg gctacggcac agctggatga ttacaagaag ctgtatctca
    12481 aggaccgcga gatcaaaaat atcatcatcg tggacgatta cctgtctccc tgggcggtgc
    12541 gcaaggctca cgagcgggga gaagtcaatg ccgtagtgga tcgcaaggca ttttcccagc
    12601 tgatggaagc ccttcgtacc aaaggcacca cggagctggc gaagcggatg gatattgcgg
    12661 aggagaaagt gcctctggtg tacatcagtg ccattctgac cagacgaatt gcagaactca
    12721 tgggcgcgga actggtctgg gcaccgggcg taacactctg cgacggcatc gcctatgagt
    12781 atgcggagca gaacaaaatg ttccgtggcg agcatgattt tgcggaggat attatcgcct
    12841 gcgcactgaa tatcagcaag cgctacaatg gcagcagtaa gagagcggat accctggaac
    12901 atatcactac cacgattttt gacagtatga aaaaagtgca tggcatgggg cagagagaac
    12961 gcctgtatct gcggctggcc gctattttgc acgactgtgg aaaatatatc agcctgctca
    13021 atgtcggcga ggcttcctat gacatcatca tggcaacgga aatgatcgga ttgtcccata
    13081 tggagcggga aatcgtggcc aacgtggtaa ggtttaacca cagcgatttc gtctattatg
    13141 ggcaggcaca ggagcgccct atgggactgg ataaggcgag ctacttaacg gttgccaaac
    13201 tgactgccat cttacgtctg gccaacagcc ttgaccgcag ccacaaacag aaaatgaaag
    13261 gtgtcaaggc cgttttacag gagaatgaac tgattctgaa ggtggacacc caggaagata
    13321 ttacgctgga aaagggattc ttccagacca gcacggaatt tttcaaggaa gtatacagca
    13381 ttaccccggt gatccggcag aaaaagaaat tttaagggag gcagcaggga aaatggaaat
    13441 cgattttaaa gatccgaaat attataccaa ccgggaactg agctgggtac tgttcgacta
    13501 tagagtgttg aacgaggcca gagacaagag catcccactg tttgaacggt tgaaattttt
    13561 aagcattaca gcatcgaacc tggatgaatt tttcatgatc cgggtggcat ccttaaagga
    13621 tatggaaaat gcgggctata ccaaaaagga tattgccggc atgacgccca cagagcagtt
    13681 aaaggcgctg catgtagcaa tccatgaact ggtggatctg caatattcca cctataaccg
    13741 ttccttactc cctcttctgg agaaaaacgg gctgcatatc atcagagagc atgagcagct
    13801 gacggcggag gaggcaacgt atgtggatca gtattttcag gaaaatgtgt atcctgtgct
    13861 gacccccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc aacgtatgtg
    13981 gatcagtatt ttcaggaaaa tgtgtatcct gtgctgaccc ccatggccgt ggactcttcc
    14041 agaccattcc ctctgattcg caacaaatct ctgaacatcg gagctatggt gcggaagaaa
    14101 aacagtgacg aggaactgga attcgccacc gtacaggtgc ccagtgtcct gagccgtgtg
    14161 gtacggatcc cgtctaaggg caaggcctgc aagatcattc tgttagaaga gatcattgag
    14221 cggaacatgg ataagctgtt cctgaattat gatattgtct gtgcccatcc tttccgtatc
    14281 atgcgaaatg cggatctgag cattgatgag gatgaggcgg cagacctgtt aaaggagatc
    14341 gaaaagcagt taaagaagcg tcagtggggc gaagccatcc gcctggaagt ggaaagcggc
    14401 atggataagc ggctgctgaa gatcattaag aaggaattga acatcgagcc cgagaatatt
    14461 tatgaaatcg acggaccgtt ggatctgact gtattgatga agatctacgg gctggagggc
    14521 tttgaccatt taaagactcc caagtatgag ccccagcagt caccggagct tcccgcaggc
    14581 tgcaatattt tcgaggagat ccgtaaggga gatatcctct tacatcatcc gttccagagc
    14641 tttacgccgg tggtggattt catccgtcag gcggcgagag atccggaagt actggctatc
    14701 aaacagacct tgtatcgtgt cagcggcaat tctcccatta ttgcggcatt ggctcaggcc
    14761 gccgagaacg gcaagcaggt cacagtactg gtggaactga aagcccgttt cgatgaggaa
    14821 aataatatcg tctgggcgag aatgctggaa aaagcaggct gtcatgttat ctacggactg
    14881 gtgggattaa agactcattc caagatcaca ctggtggtcc gcagagaaga ggatggcatc
    14941 cgcagatatg tccatctggg taccggtaac tataacgatg ctacggcgaa gctgtacacg
    15001 gatgtgggta tgctgacctg cgcggagcgc atcggcgagg atgccacggc agtattcaac
    15061 atgctgtccg gttattctga gccactgttc tggaacaagc tggcattggc accgttatgg
    15121 ctgaaggata agttcctgca cctgatcgag cgtgagaaaa attatgcgct ggagggaaag
    15181 aatgctcata tcattgcgaa gatgaactcc ctctgcgaca aggatattat ccgtgccctg
    15241 tatgaagcaa gtgcggccgg cgtgaagatc gaactgatcg tcagaggtat ctgctgcctg
    15301 aaggtgggaa tccccggtgt cagcgagaat atcagtgtcc gttccatcgt cggcaatttc
    15361 ctggagcata gccgtatttt ctattttgca aatgatgatc attatgagat ctactgtgga
    15421 agtgccgact ggatgccccg taatctggag cgtcgtgtgg agatcctctt cccggtagat
    15481 cgcccggagc tgaaggaaga gttattacat attctgaaca gtcagctgaa ggataatgtg
    15541 aaggcttcta ttttacagcc ggatggcagc tatgagaagc aggacaagag aggaaagcag
    15601 ttgttcaatt cccaggatgc gttctgcctg gaggccattc aaaaagcaaa ggaggctgcg
    15661 ggagaatctg ccatcgaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
    15781 gcctggaggc cattcaaaaa gcaaaggagg ctgcgggaga atctgccatc gaaggcagaa
    15841 cttttatccc ggagacacat catgaataag aagatcatac tggcatccgc ctcccccagg
    15901 aggcgggagc ttctgacaca gatcggactg gatttcgatg ttgtggtcag cgagaccgag
    15961 gagaagatca actccacaga gccggcgaaa gtggtggaag aactgtcagc gcagaaggcg
    16021 gaagcggtgt gggagaagct tgcggtaagt ggtgtctttg atccggaaca gaaaagtgga
    16081 gaaactacta tgacagacac tcttgtgctg ggagccgata ccgtggtcgc ctgcgacggt
    16141 aagattctgg gtaagcctgc ggatacggaa gctgcggcag ccatgcttac tatgctgcag
    16201 ggcagaggac atgaggtata cacaggcgtg acgatcctct atgagcaaaa tggtgagagg
    16261 aagacgctga cttttcatga gaagaccata gtgcattttt atcccatgac cgatgcacag
    16321 atccgggaat atgtggctac cggggatccc atggacaagg cgggatccta cggcattcag
    16381 ggattctgtg ccagatatat ccgaggtatc gaaggtgatt acaataatgt ggtagggctt
    16441 cctgtgggaa gagtctatca ggagttacat ggattgagat tagtgtagga gaacagaatg
    16501 aagaaagcag caatttttga cctggacgga accttatccg attctattat gagcatgaaa
    16561 tacagcggag acaagaccat ggcggaattc ggctatggac ctttctcggt acaggattat
    16621 aaatattttg tgggcgacgg cgctgccaat ctggtaaagc gggcactgat caagagcgga
    16681 gataccggac tgacccattt tgaggcagcc tatgccagat acaaagagat ctttcaggcg
    16741 aactgcatgt atcaggtgaa gccctatgag ggaattacgg aactgctgga agagttgaag
    16801 aaacgtggaa taaagatcgc cgtattatcc aataagcccc accatgccac cgtggatgtc
    16861 attgagagtt tatttggtaa gggctatttt gatgtagtgc agggacaggt ggacggcgtg
    16921 cccatcaagc ctgatcccgc cggagtcttc cgcatcttag acaagttcgg tttcaccgca
    16981 gacgaagtac tctacatggg agataccgct acagacatga agaccggcaa agccgccggg
    17041 gccttcaccg taggcgtcct ctggggcttc cgtgaccgca aggagctgga agaaggccac
    17101 gccgatgcta ttatcgccca cccgttggaa ttgttgaagt attgcgaata attgaaagtt
    17161 agcatagcta cgagccatgc aaaaatagag tggaacacct cgatgggcgc t
//
LOCUS       DAWEJW010000060        16843 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1281_length_16843_cov_58.506552, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000060 DAWEJW010000000
VERSION     DAWEJW010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16843)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16843)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16843
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_16843_cov_58.506552"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7231..7330
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14239..14338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4650 a         3587 c         4443 g         3963 t
ORIGIN      
        1 aaggtagctc tgttagacct gggagccaag agaaatatcg ccagatccct ggcagagaga
       61 ggctgtgagg tgaccgtata ccccgctgga actcccgcac aggagatcat tgatgataat
      121 cccgacggta tcatgttaag taacggaccg ggagacccca aggagtgtac cggtgtgatc
      181 gccgagatca aaaagttata cgataccgag attcctattt tcgcaatctg tctgggacat
      241 cagctgatgg ctctggctac cggcgcagat acccacaaaa tgaaatatgg acacagagga
      301 ggcaaccatc cggtaaagga tctgatgacc ggaagagtat atatttcttc tcagaaccat
      361 ggatatgtgg tagataccga caagttggat ccttccgtag cggttcccgc atttatcaat
      421 gtcaacgacg gtaccaacga gggtctgtcc tacaccggca agaacatttt caccgtacag
      481 ttccacccgg aagcatgtcc gggaccccag gactccggtt acctgtttga ccggttcatt
      541 catatgatga gaggagagaa ctaagatgcc caagaatcct aatgtaaaac gagtaatggt
      601 aatcggctcc ggtcctattg tcattggaca ggcagcggaa ttcgactatg caggtaccca
      661 ggcctgccgt tccctgaagg aagagggagt agaggtaatc ctggtgaact ccaaccctgc
      721 gaccatcatg actgataaag atatcgcaga taaggtatat atcgagcccc tgaacgtaaa
      781 ggttctggag cagatcattg aaaaggaaaa gcccgacagc atccttccca ccctgggagg
      841 acaggctggt ctgaacctcg gtatggagct ggaggagtcc ggtttcctgg ctgctcataa
      901 tgtaaagctg ttaggtacta ccgcagctac cattcgtaac gccgaaggac gtcaggagtt
      961 caaagacctg atggagcgca tcggtgagcc ctgtgcagca tccagggtcg ttgaaaatgt
     1021 ggaagacggt gtggaattca ccaataccat cggctatccg gtcgtactgc gtcccgctta
     1081 taccctggga ggttccggtg gcggtatcgc ccacaatcag gtagaactgg aagagatcct
     1141 ggagaacggt ttacgtctgt cccgtgtggg tcaggtactg gtagagcgtt gtattgcagg
     1201 ctggaaagag atcgagtacg aggtaatgcg tgacagcgca ggcaactgca tcaccgtatg
     1261 taacatggag aacatcgatc ccgtcggcgt gcataccgga gacagtatcg tagtagctcc
     1321 ttcccagacc ctgagcgaca aggagtacca gatgttacgt acttccgctc tgaagatcat
     1381 caacgaactg cagatcaccg gtggatgtaa cgtacagttt gcactgcatc ccaccagctt
     1441 tgaatattgt gttatcgagg ttaaccctcg tgtcagccgt tcttccgcac tggcaagtaa
     1501 ggcgaccggt tatcctatcg caaaggtagc agcgaagatc gcactgggct ttaccctgga
     1561 tgagatcaag aacgccatca cccacaagac ctatgccagc ttcgagccga cactggacta
     1621 ctgtgtcgtg aagattccca gactgccttt tgataaattt atcactgcaa aacgtactct
     1681 gaccacccag atgaaagcaa ccggtgaggt tatgagtatc tgcaataact tcgagggagc
     1741 actgatgaag gctatccgtt ccctggagca gcatgtggac tgcctgcgca gctatgattt
     1801 cagcgctttg tctgtggagg aactgttaga gcgtctgaag atcgtggatg accagcgtat
     1861 ttatgtcatt gcggaagcta tccgtaaggg catcagctac gagcagatcc acgacattac
     1921 caagatcgac ctgtggttca tcgacaagat tgccattctg accgagatgg agcatgcgct
     1981 ggagacacag ccgctgacgg ttgatctgtt gaaggaagcc aagagaatcg agttcccgga
     2041 taacgtgatc gcaagactga ccggcaagac cgaggaagag atcaagaaga tgcgttatga
     2101 caacggcatt aaggctgtct acaagatggt agatacctgt gctgcagagt tcgcagcttc
     2161 cactccttat tactattccg tattcggcgg cgagtgcgaa gccaaggaga ccaccggacg
     2221 caagaaagtc ctggtactgg gttccggtcc tatccgtatc ggacagggta tcgagttcga
     2281 ctattgttct gtacatgcta cctgggcatt ctccaaggca ggctatgaga ccattatcat
     2341 caataataac ccggagaccg taagtaccga cttcgatatt gcggacaagc tgtattttga
     2401 gcctttgact ccggaagatg tggaatctat cgtaaatatt gagcatcccg atggtgctgt
     2461 ggtacagttc ggcggacaga ccgctatcaa gctgactgag agcctgatga agatgggcgt
     2521 gcctatctta ggtaccaagg cggaggatgt agatgccgct gaggacagag aactgttcga
     2581 caagattctg gaagagaccg ggatcccccg tgcagccggc ggtactgtat acaccgcaga
     2641 ggaagctaag gcggttgcta acagactggg ctatcccgta ctggtgcgtc cttcctatgt
     2701 attgggcgga cagggcatgc agatcgctat ctccgatgaa gagatcgaag aattcatgga
     2761 gatcatcaac cgtatcgcac aggatcaccc gatcctggta gataagtatt tacagggtaa
     2821 ggagatcgag gtcgatgccg tatgcgacgg taccgatatc ctgatccccg gtatcatgga
     2881 gcatatcgag agaaccggta tccactccgg agacagtatc tccgtatacc cggctccgac
     2941 cattgataaa gtagtaaaag agaagatcgc agagtacacc agaagactgg caaaggcact
     3001 gcatgtcaag ggactgatca atatccagtt cattgccatc ggtgacgagg tatatgtcat
     3061 tgaggttaac ccccgctctt ctcgtaccgt accttatatc agtaaggtaa ccggtattcc
     3121 tatcgtggat ctggcgaccg aagtgatcat cggcaagacc atccgtgagc ttggctacga
     3181 gcccggctta cagcctgagg cagagtattt tgccatcaag atgcccgtgt tctccttcga
     3241 gaagatccgc ggtgccgaga tcagcttagg accggagatg aagtctaccg gtgagtgtct
     3301 gggtatcgcc aagaccttca atgaggctct gtacaaggca ttcctgggag caggcgtaga
     3361 actgcccaag tacaagaaca tgatcatcac cgtgaaggat gcggataagg gtgaagccat
     3421 tgaagtcgga agacgcttcg aaaagctggg atacaccatt tatgctacca gaagtaccgc
     3481 agctgctctg aatgaagccg gtgtgaaagc aagaaaggtc aacaagatct ctcaggaatc
     3541 cccgaccgtt atggacctga tcctgggaca caagatcgac cttgtcatcg ataccccgac
     3601 ccagggccgc gacaagacca gagacggttt cctgatccgc cgtaccgcta tcgagaccgg
     3661 tatctactgc atcactgcaa tggacaccgc caatgctctc gtgaccagca tggagcatat
     3721 gaacgattgc aaggaactga cgctggtgga tattgcacaa ctgtaagata taaaaatgga
     3781 taagaacagt ggagtcgcga aagttgctcc actgtttgtg tttgtgacaa aaacaaagtg
     3841 ttgacaaatg tccgcgaaag gaataaaata caaacagtta aagacataaa ggctgtgaag
     3901 agaagagtag gagaagagat acgtcgcaga gagtcccggc aggtgggaag ggatatggaa
     3961 ggcttctttg aacatggtct cggagctttg caccgacttc gtattcggac aggatatccg
     4021 gcagatgcgg acaggctgca acgggagtat ccgttatcgt acctgactat cgatgagcgt
     4081 ttgtttgtcc gtagttgcag aggcctgatc ccgtgagaga tgggcgaatt aaggtggtac
     4141 cgcggaagaa tgttccgccc ttaaatttta atttaagggc ggttttttat tatgtcaaaa
     4201 agagaagaac tgatcagaga atattgcagg caggtaaatg aaagcctgca ttttatacaa
     4261 aaatacatgg aagggatcgt tcccgctggc cgatgcagcg aagaactgta ttgggagacc
     4321 aagaagatca gtgaggaact gcagaagagc aatcatgaac agaccgcatt gatccgggcc
     4381 tatctgcggg aggcggcaca actgtacgta gagggggagc cctggaagac ccgcattacg
     4441 gaccgaagac tttgccgtaa tacgatcctg aatcatttac agatgctggc gaatgtcagc
     4501 ttctggctga gagaacaggg agagccactg gcagaggaag tctgcggatg gctgttagcc
     4561 caggagacag cggatgtgca gaaagttaga tccggacaaa gtctggaagt tgccagagag
     4621 atctatccgg ccgtgcagag aaagagagca taggagcggt cggctgtaaa agcacaaatt
     4681 tgaaaataaa atccgtgggg acagcatata ctaggcgtat ggtaaatatt gtatgatcgt
     4741 aagcctgtat cccgcaggcg gattggagtc tcatgttaga agaacattat ccttttgttg
     4801 cgcagcctct tccctacgaa tatgatgccc tgcttccggt attagatgag gaaacacttc
     4861 attttcatca cgacaagcat tatcagacct atgtggataa gctgaatgcc acccttgccg
     4921 attatccgca gttacagcag atgtccttga cggaactgct cacttcggag gataatatac
     4981 ttgcgtccct gccggaggaa gcccggaaaa gcattcacaa taacggtggc ggtgtatata
     5041 atcatcagct gtattttgac agtatgcgaa gcccggcagg acaggagccc tgtggagctc
     5101 tggaggaggc gctggtccgg gattttggat cggtgagaca atggaaagaa cagatgtcac
     5161 aggcagccac gggggtattt ggctctggat gggcatggct tgtgagtgac caggacggta
     5221 ctctgatagt ccttactaca gcgaatcagg atgtcccgga tctgaaattg tatacctcca
     5281 tatttcccat agatgtgtgg gagcatgcct attatttaca gtatcagaac cgcaggccgg
     5341 attacgtgga aggatggcat aagctgatcc actggaaaaa agcgggacga cgttacgaac
     5401 aggtactttg cagactggaa agagcggctg gggaagcgga aaaattgcaa aatgacagcc
     5461 gcacaacaga agcgaacgac agatagtgtg caacgtgcgg aacagaagaa cagaaagagg
     5521 aaaatgacta tggagaacag accgatcata aaaaaggaac aggtagagac cgtctacgac
     5581 agcagattta tcaaggtatt tgatctgcaa tatgagccgg gaaaacatta ttttgatgct
     5641 accagaagag ccaaagataa tctggcagcg gtaaagagcg acgaagaatt cagacagatg
     5701 ctgccggatg cagtcagctg cgttgtgatc ctgaagatca gaggacaaga accgagactt
     5761 tgcttaagcc gtgagtatcg ttacccggca ggacattttc tgctgagcgt tccggcagga
     5821 cttttagacc cggaggatgc aacggaagaa aatcccgtat tccatgcggc agcccgggag
     5881 cttcgggaag agaccggcat cacactggaa gagagcgaca gcatccgtct ggtgaatccg
     5941 ctggtattca gtacccccgg tatgacggat gagagcaatg cactggtgca gatcatattg
     6001 aaccgtgaag agatgccgaa ggtaagtcag gaaggcgccg ttggaacgga atgttttgat
     6061 ggttttctgc tgctgaccaa agaagaagca cagaaaatct taaaagacgg cgtggatgac
     6121 cagggatttt tttacccgtt atatacctgg gcggcattga tgtgctttgc gacgggaatg
     6181 tggaaataat ttttgaataa ataagacgat gaacgaaaag cgtgtggaga gatctgcacg
     6241 cttttttgaa atttgtaaca aatagcgaaa aattcttcgt tatgattaac aaagaggaag
     6301 gttgccgttc gctcaattga ttttccaatt gctcaattgt ataattatgt cattagcaag
     6361 tacaattgag gagcaaagac atatcctgtg atgaattgct gaatccggaa aagacattgg
     6421 agagattagc gaatccagaa ccgcagaaag gagagacaga ggaaacggac ggagagcccc
     6481 cgaagaaaaa gaacagcctt atagtaaaaa ctgctgtcct ggtgggaata ctggtgatcg
     6541 tcggaggact gctgttggga tatatgataa agcatagaga acctacttat cagcagatag
     6601 gcacaagata catcgatgat cctgactggg ggaatgtatc tgaaatttcg tatgtggtaa
     6661 aaggggaagt aacggcagag agacttaatg agcatttgcg tgaagtgaga gaaacggtgg
     6721 accgggaaga actgggtacc aatgttgtca agactgtata ctacaggaac aaggaagacg
     6781 ccctggcgtg gaaggatacg gatatgggag gatatacttt cctgaatgta gaatgaaagg
     6841 agcagctatg aagaagattt tttcaaatta ctacaaaatt attttgtctg ttgtgatcct
     6901 gattgtcctg gtggcactaa aggaggatta caaaacttct tttgacgaaa tgagcggagt
     6961 ccggacggtg gtacgtatgc tgctctatac ggcatgggga ttcctgttgg cgacaaggac
     7021 cagacgggac aaagcaaaag gtgcggaata tgttgctttt ggcgttgcct gtgtggcagc
     7081 gctcaatctg ttaatcccca tctggagctt ccagatagcg ttttatgctt ttgtcgcagg
     7141 tgttccggga ctctatttta tggtatttat tctgaccgga atattaggag gttacttatg
     7201 gggaccgagg ggttaccgca ttagggatga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn attagtgatt gctttgaaaa ataaatattg acatatatag atagccgata
     7381 tatattatag acaacgatat atatcggcta tctatataag gaggacaaaa aatggctgtg
     7441 gacaaatcac tgatgtcggg aagtatgacg atgcttttgc tcagattact ggcggagaag
     7501 gatatgtacg gatacgagat gatcgataca ctgcggaaaa aatcctgtaa cgtgttcgag
     7561 ttaaaagcag gtacgctgta tccgttgttg catgggctgg aagcgaaggg cctgcttacg
     7621 gtatatgagc aggaatatgc aggcaagacg aggaagtatt acagtattac gaaagagggc
     7681 agaaaattac tggaaaccaa aaaagcggag tggatggaat atcagtctgc tgtgaccagc
     7741 gttctggcaa tgggggtata aggatggaaa catatttgga aaaactgttg tcacagatcc
     7801 gttgtaaaaa agccagaccc tatattgctg aggagataag agaccatata gaatgccaga
     7861 ttgcagacaa tctttccgaa gggatgacct ctgaggaagc agagaaaaat gcggttgcgg
     7921 acatgggaga tccggtggaa gtcggtatct cactggacag aatccacaag cctaagattg
     7981 catggaggtt acttgtgatc gtaggaattt taagcctgct gggcattctg atacagcaat
     8041 ccattctcag acaacccggt tatcaggagc tggaaacatg gagacaggaa gtgtaccgct
     8101 atactacgga ggggtttggc tcagccgttg ctattggatt cctcctcatg tgtgtgatct
     8161 acttcctaga ttataccgta attgcaaagt actccaggtt catcggagga gctattctga
     8221 ttctgggagg gctgcgggtg gcaggcttcg gtggtctgga tgtcaacgga atcggcaact
     8281 ggatcggatt tggaaggctc cgggtggcgg ttacttccct gatgatgttc tatgtgccga
     8341 tttatggcgc tatcctatat aaatacaggg acggtggtgt atccgcactg tgcagggcga
     8401 tcctgtggct gatccttccg gtatttatta catcccgaat ccccagcctc ggtgtggctg
     8461 tcatcatgat ggtcagtatg ctgatagaac ttacggtggc tgtctggaag gggtggtttc
     8521 aactgcctgt gaaaaagacg attatcggtg tgtggctgtt tttcgcagca gtacctgcta
     8581 tcttactgac tgtaaagtac gcatttcata tgctggaaac gtatcaggaa gccaggatcc
     8641 gttcctattt atcccattcc ggagatgcca attacatgac ggccacgctt cataaattta
     8701 atgaaaatat tttactttgg ggcaacagtg gaaaagatgt ggtgggaggt ttgccggaat
     8761 tcaatcagga ttacattttc tcttatattc tgaacagtta cggactgctg gccggaatct
     8821 ttgtagccgc gatcctggcg gcactggtag tgtttctgtt cggagcgtcg gtcagacaga
     8881 aaaatgaact aggcatggtg atgggattcg gatgcggaat gatcatttta ttaaatattt
     8941 ctctgaactt tgcgggaatg ttgggatgga tcccgctgac atcgaccttc ctgccgttct
     9001 tatcctttgg cagaaataat attttactgt gctacgcact ggtgggaatt atcctgagta
     9061 tttacagata caaagatgta tatccaaaaa agttcaaggc gtcacaggta tcattgcaga
     9121 agaccattac tttgaatctg aatatgtaac atggtaataa aagtaatgca aataaaaggt
     9181 ccatgagcag gaaaactgtc catggacttt tatatatcgt gagcagaact cactcaatgc
     9241 atttcgcaag cttacgaggg caataccgac aaaatcagta tctccacgct catcatatca
     9301 ttcatccgcc cggatttcac ggcttcctcg gtctctacac actgctttac cgcatttttc
     9361 agcacggaag tcttgaaatg ggatgcctgt gccacatatt ttccggcggc aaagggcggt
     9421 acaccaatct tggaggcaat ggtcttattg tcatggcctc tgctttttaa ttccttggtc
     9481 tggagcagca ggttacactg cctggcgatc aggaacagca cccgcatggg cggctccttc
     9541 agtgccagca gatcgtaata gagctgcaag gctttctgcg gctgcttttc cgcaatggca
     9601 ttgaccatat cgaagatgtg gttggtgatg cgagtggtgc agaccgcttc cacatcctct
     9661 gcggtgacta catcccggtc cagacagtag cagatcagtt tttccagttc catctggata
     9721 ttttccatgt ccgtgccggt tttggagagc agcaactgtg cggtgttctc ggagattttt
     9781 ttcccttccc ggcccaggat accggcaatc cagcgtttca gggaattctc atcctgtacc
     9841 gtgaactctg ctgcgtagcc tttggactgc acggctttgt agagcttgct gcgcttatcc
     9901 acttcacttt ccgtgaacac gaaaaaggtg gtgtcgttgg ggcttgccag gtattccgcc
     9961 agtttttccc caccggattt gaagagcccg gtatctttga aaaacatgac acggcgttct
    10021 gccagaaaag gcagcgtctc tgccagatcg atgacttgtc ccaaggagaa atccttcccc
    10081 tgatagaaat gggtattcat ctcgtcgccg tcctgacaca acgctttacg cagacgggtc
    10141 gtgtactggt ttttcagata acgttcctct ccgtagagca gatagatctg tttgaaattg
    10201 ccgttcttga tgtcttcgtt gatccgctgc atgaattgat cctttctggg aaaatgcggt
    10261 tacttctaca ttagattcct aaaagagtat accccatttc ctattgaggt ttcaaggggt
    10321 gtatgctatt atggaacaaa acagtttgac catggacata tacgagagac aaaattatgc
    10381 ttgcatgatt ttgcgaacga atatgtccat gagttaagcg tcgattcatg agcaaaggta
    10441 gtcacgaagt gacggaaagc gcggctcgac cttcatacca taagaggaag gaagagaaaa
    10501 atgagcaaga aaagcggatt cattcaggaa tttaaaaagt ttattttgag gggaaatgta
    10561 atggatatgg cagtcggcgt gatcgtcggc ggagcattca ccgctatcgt tacttctctg
    10621 aatcaagata tcctgacacc gattctggcc atcttcggag gcacggattt ttcgaattta
    10681 tatataaagc tgggaagcgg cgatacggca ccggtattga tgtacggtaa ttttatcacg
    10741 gcagttatca acttcctgat cacagcattt gtgatcttct gtctggtaaa attcatcaat
    10801 aagctgggag acaccctgca tcacaaagag gaagcaccgg cagcgcccac gacgaaaaaa
    10861 tgcccgttct gtaagagcga gatcgctatc gatgccacaa gatgccctca ttgtacttct
    10921 cagttagaca aataggaacc ggtaatacag atacggccgt ggaggaaagc atgggaaaaa
    10981 tacacaggaa aatagtggca gcaggagtct gctgtctgat gacaggcatg ctgacaggct
    11041 gcggaagtga taccacgaag atcaccgaag gaatgcagtt ggtggaaact ctggattatc
    11101 agggggcatt gaatgccttt gacgaagcgg aagcacagaa agaggacagc cgtctgatcg
    11161 caagaggccg gggaatcgcc agcatggggc tgacagagta tgatcaggcc gtagaatatt
    11221 ttacacaggc tctggaactc agtgacggct gggttcagaa cgtggactat gatatgaatt
    11281 actatctggc ggcggcatac accaagaacg gacagcctgc ggaagccaaa aaggtatacg
    11341 atgccattct gggcctgaag ccggaggaaa aggacgctta ttttctcaga ggttctgcag
    11401 aactggagct gggagattat gagagtgcca aagcggattt tgatcaggca atctccatgg
    11461 atccgaaaaa ttatgaccgg ttaatagaaa tctatgaagc cctggcagcg cacggctacc
    11521 gtgaggtcgg tcaggaatat ctgcaaaatg tactggacac agccaaacaa ctggatgctt
    11581 atgacagcgg acggatctat tattatctgg gagaatacca gaaagcttat ctggcgctgg
    11641 atgaagcccg ggataaggga ggcgcggaca gctatctcta tctgggaaga gcctatgcgg
    11701 ctaccggaga ttataactat gcgtccagtg tgtacaacaa ttatctctcc aagcaggggc
    11761 cgaatgcaga aatctacaat gaacttggtc tgtgcgaaat ggcaaaaaag gagtatcaga
    11821 atgctctggc ggcatttcag gcgggaaaac agatcgaagg aaacagcctg atgcagacct
    11881 tgtgctttaa tgaaattgta gcatatgagt atctggaaga ttatcagcag gcagaagtac
    11941 tgatgaatgc ttatttacaa aattacccgg acgaccagac agccatccgg gagcaacagt
    12001 tcctgtccac cagataaaaa gacaatagaa tttatggatc aatcattggc agagtcttcc
    12061 cgggaggaag attctgcttt ttctcttaat tgcagcagat ccacaaaatc cagcagagaa
    12121 gccatgtttt tcggattaaa atgctgtatc acctctacca catcggatac ggtggtgccg
    12181 gggacacatt cctgtctcag aacttccgga ttgtaacgat aaggagtccg acccaacaga
    12241 taatcgatgg ttacatcgaa atattccgct aattgtatta aagcttcctg atccgggaag
    12301 tgaatattgt tctcgtaatt cgaaatggtg ccaacggata ctttcagtat ggcagccagt
    12361 tctttctgat aaatgccccg ttcttcccgc agggatgcca gaatttcacc gaaactcctc
    12421 ataatgcctc gcattctgcc gcagatgcag cctatttaaa tgttttcttt taagatttga
    12481 taaattctat tttactatat tttagtttta aaggaatatg aatttagaaa tcgaggaaaa
    12541 taatgaaaaa atgctactag aattaatttt aactaaaaag tacaaattat gcaggaaaat
    12601 atccattgag aaatcctctc attaacacct attgctattt tgaagaaagc cggatataat
    12661 aatccaagag ttaggcgcaa ctaactaatg agtgaatcat gtgaaactcc tcagagaaaa
    12721 cggagctgct gaatccgcgg actgccggga ggtcatcttc cgggaggagt ttttcagccc
    12781 ttatacacag ggatgaggga ttcacgaaag agaaggtttg ggaataaaaa gtttagaata
    12841 gaggataaag gagaagaaca agatgaacta tttagagatt gaaaaagtca ttggacgaga
    12901 gattctggat tccagaggca atcctaccgt agaagcagag gtaacgctgg tagacggtac
    12961 catcggacgc ggcatggctc ccagcggagc atctaccggt gagttcgagg cactggaact
    13021 gagagacggc gataagagca gatatctggg caagggtgtt cagaaggctg tagaaaatat
    13081 caatactgta atcaatgaga ctctgcaggg aatggatgca tccgatattt atgccattga
    13141 taaggcaatg atcgatgcag acggaaccaa ggataagtcc agactgggag ccaatgcgat
    13201 cctggcagta tccattgcca gcgcgagagc agcagcaatc tccctggata ttcccctgta
    13261 tcgtttcctg ggcggtatct ccggcaacag acttccggtt cctatgatga acattatcaa
    13321 cggcggctgt catgcactgt cctccggcct ggatgtacag gagttcatga tcatgcctgt
    13381 aggtgcgcct tcctttaagg aatgcttaag atggtgttct gaggtattcc atgcactggc
    13441 ttctattctg aaggacagag gcctggctac ttccgtaggt gatgagggtg gttttgctcc
    13501 ggcactgaag tctgatgagg aagctatcga gaccatctta gaggctgtaa agaaagccgg
    13561 ttatgagccc ggcaaggatt tcaagatcgc tatggatgct gcttccggcg agtggaagac
    13621 cggcaaggtg ggcgagtaca agctgcctaa ggcaggcacc gtatttacct ccgaagaact
    13681 gatcgcacat tggaagaaac tggtggagaa ataccccatc atttctatag aagatgcact
    13741 ggatgaggaa gattgggaag gctggaagaa gctgaccgca gaactgggcg acaaggtaca
    13801 gttagtcggc gacgacctct tcgtaaccaa caccgagagg ctggcaaagg gaatctccct
    13861 tggctgtggt aactccattc tgatcaagct gaatcagatc ggatccgtat ccgagactct
    13921 ggaggccatc aagatggcac acaaggccgg ttataccgct atttcctccc accgttccgg
    13981 tgagaccgag gataccacca ttgccgacct ggcagtggca ctgaacacct gtcagatcaa
    14041 gaccggcgct cccagcagaa ccgagcgtgt ggcaaaatac aaccagttgc tccgtatcga
    14101 ggaagaactg ggtgcagcag ccgcatatcc cggcatgagc gcattcaacg ttcagaatta
    14161 atttgctttt acccaaaact attttggttt ctctttttct cttcagggac tgttcgaaag
    14221 gacagtcccg tttgcgttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
    14341 cgtaagatcc cgggaacagc cgaaggaagc cgcggaagaa aaaatacatt atttcatatt
    14401 gacttttccg aaacctatgt gataaaatct caccgttgcg aaaatagcat agagacttta
    14461 ggtaagaatt ggaaacgaca catgtgcgca ctgctcactg attaacgaac aacacacatg
    14521 tgtctttttg cgcccatttt tagaaaagga gggcattaat tcatgccgaa aacaagatta
    14581 cagaacatta ttttcaccct gatcatggca ttcgttatgg tatacgccat ggtctgctac
    14641 aacatagcac tggacaaagg aggaatgacc aacgagatct tcctgttagc gttccacgaa
    14701 attcccatca tgtggcccat tgcctgcatt ctggagtatt tcgcagtgga aaagctgtcc
    14761 cggaagctgg cattccgcat ggtatccccg gaggataagc ccgttttcat tactttagcg
    14821 atctcttcca tgatcgtatg cctgatgtgc ccggtgatga gttttatcgc cacctgcctc
    14881 ttcatgcacc ccggcaacca gatcatcgcc ctgtggctgc agaagaccgt ccagaacttc
    14941 cccatggcgc tgtgctggca gatctttttc gcaggccccg gagtgcggaa tgtctttgga
    15001 tttgttgtgg gaaaaattta tattggatag aaaaagtata attgactatc atctgtgagt
    15061 gtgctaacat gagcaaataa agagacaacg aagtccgtgg aaacggaaag cgttgtctct
    15121 tttattttgt agaaaaagtc aggaaattac gaggtgaaac ctatgagtga gcaggaaaag
    15181 aagcaaaata cagttgcaga ggtgttagta aaatgtctgg aaaacgaggg cgtgaaatat
    15241 gtcttcggga ttccggggga ggaaaatctg gatatgatga atgcactgga aaaatcggac
    15301 atccgggtga tcgtggtgcg gcatgaacag ggagcggcat tcatggctga tatgtacgga
    15361 agactgacgg gaaaagcagg tgtgtgtttc tcaacgttgg gccccggtgc cacgaacctg
    15421 atcacaggca cggcagatgc taattccgac ggtgcaccgc tgattgccat taccggacag
    15481 gtgggaacgg agcggatgca tttgacttct catcagtatc tggatctggt agagctgttt
    15541 gcaccaatca ccaagcggag caaacagatc gtgaacccca attccgtcaa tgaaatcgtc
    15601 aggatcgctt ttaaatatgc ggagagtgag aagccgggag cctgtcatgt ggatcttccc
    15661 acgaacgtgg cgaagcttcc ggtcagctcc ggtattgccg cacagccttt gaaaaagccg
    15721 gagagggata aggagtacgc atccttttcc agtatcgatc aggcggcggc catgatcttc
    15781 aaggcaaagc acccggtgat cctggtggga cacagtgcgg tgcgcaatca cgccggggag
    15841 gctcttacca gcttcgcaga tgtcctgaag atcccggtgg tcaataccat gatggccaaa
    15901 ggcatcattc cttataacaa caaatattcc ctctggacca taggcatccc ccagaaggat
    15961 taccagaaca aggtgctgga tatggcagat ctggtgatcg ccgtcggtta tgatattgtg
    16021 gaatttgctc ccggcaaatg gaacggagag gggaaacata agattattca tatcgaccag
    16081 aggccggcgc atatcaacat gctgtaccag ccggaggtgg aagttgtcgg agacatttcc
    16141 tattctttac agcagatcct gtaccgcagt gacgcaaaag aggagccgga agaatttctc
    16201 aaactgcggg aagaaatggt ggcggaatat gaaagctatg cagatgacac ctcttttccc
    16261 atgaagccac agaaaatact gtacgatgtc cgtaaattta tgggtgcgga tgacatcgtg
    16321 atctcggatg tgggcgctca taaaatgtgg attgccagag agtacaactg ttacgaaccc
    16381 aatacctgca tcatctccaa cggttttgcc accatgggca tcgcagtgcc gggggctgtg
    16441 gcagccaagt tgatctaccc ggagaaaaag gtactggcga tctccggaga cggcggattc
    16501 atgatgaata gccaggagta tgagacagcc ctcagagagg ggacacccat tgtgactctg
    16561 atcttctccg atgccagcta tggtctgatc aaatggaagc agatggatca cttcggacac
    16621 aactgctttg tggatttcca gaatcccgat tttgtgaaat atgcggaaag catgcatgcc
    16681 aaaggctacc gcgtggagaa ggcggaagac ctgctgccga ttctggagga tgcctttcag
    16741 cagaaggtcc cctgcatcat cgactgtcct gtggactaca gcgaaaatac caagctctcg
    16801 gagtatctgc atgataagtt tgagaagtag agttttaact aga
//
LOCUS       DAWEJW010000061        16558 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1303_length_16558_cov_66.891838, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000061 DAWEJW010000000
VERSION     DAWEJW010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16558)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16558)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16558
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1303_length_16558_cov_66.891838"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4574 a         3582 c         4702 g         3700 t
ORIGIN      
        1 taagtaggaa atagaaaacg aacacaattt tcaagtataa ggaggatttc attatgagta
       61 agatttacac atccgcaacc cagctgatcg gacatacacc ccttttaaaa gtaaacaatt
      121 acttgaaaga ccagaacatt gaaggtgtag aacttctggc aaagctggag tatttcaatc
      181 ccgcaggaag cgttaaggac agaatcgcac tggcaatgat cgaggatgct gagaagaagg
      241 gcatcttaaa ggaaggctcc accatcatcg agcccacctc cggcaacacc ggtatcggtc
      301 tggcaagtgt agctgctgcc aagggctacc ggaccatttt aacccttccc gagaccatga
      361 gcgtggaacg tcgtaacctc ctgaaagctt atggcgcaga actggtactg accgaaggtg
      421 ccaaaggcat gaagggtgcc attgccaagg cagaagagct ggagaagtcc atcccgggag
      481 ccgtgatcct cggacagttc gataaccccg cgaaccccgc cgcacatgca gctaccaccg
      541 gtcccgagat ctgggaagac actgatggaa aggttgatat cttcgtagca ggtgtaggta
      601 ccggcggaac cgtgaccggc attggtactt atttaaaatc caagaacccc aatgtgaagg
      661 tcgtagcagt agaacctgcg gattctcccg tactttccgg cggacagccc ggaccccaca
      721 agctgcaggg tatcggtgcc ggatttgttc cttccgttct gaataccggc atttatgatg
      781 agatctttac cgttactacc gatcaggcat ttaccaccgg ccgcctcatc gctcataagg
      841 aaggtatcct cgtgggaatc acctccggcg cagctctgta cgcagcagca cagatcgcaa
      901 aacgtcctga aaatgcaggc aaaacagtcg tagcgctgct tcccgattct ggtgacagat
      961 acttgtccac cccgatgttt gcagaagcct aaggcatttc ctaagaactt tacagacttg
     1021 cattcttgtg ttagaatgta tggcatggta ctaggaattc aaggaatttg cgcttttctc
     1081 cccttaaaag tgcgaacctg gaatggagga acttatggaa ccgatcattc agatcaagaa
     1141 tgtaacaaaa acttttatag gtaaggataa tcaggtagag gccctgaagg gcatcagcct
     1201 ggatatcaac aagggagata tctatggcat catcggtatg agtggtgccg gaaagtctac
     1261 cctggtgcgt tgcctgaact acctggagaa gcccaccacc ggaactgttg tggtagagga
     1321 tcaggatctt tcgaccttga cagaggcgga acttcgtaag gttcgtacag gcattgcgat
     1381 gatctttcag cattttaatc tgctcatgca gagaagtgtt ctggacaatg tatgcttccc
     1441 catggagatc gtgggtgtga agaagcaggc ggccagaaag cgtgctatgg aactgctcga
     1501 gatcgtagga ctgaaggaaa aagcagacgc ttatccttcc caactctccg gtggacagaa
     1561 gcagcgtgtg gcaatcgcaa gagctcttgc caatacgccg aagattctgc tgtgtgatga
     1621 ggcgaccagt gcgctggatc ccacgacgac caaggagatc ctgaaattat tacaggacat
     1681 caacaaacag tacggcatca ccatcgtgat catcactcat gagatggctg tggtacagga
     1741 gatctgttcc catgtagcca ttatcgatgc cggagaactg gcagagcacg gtactgtgga
     1801 ggaggtattc tccagacccc agtccgcagc cgcaagaaag ctggtgttca tggtggatcc
     1861 caaggcaaag gaaatgtccg gcaaacgctg catccgcatt gttttcactg agaattcttc
     1921 ttttgaacct gtgatcgcca atatggtgct ggagtgcaag gctcccgtta atatcctgtt
     1981 agcggatacc aaggacatcg gcggtatcgc ccatggacag atgatcttac agctgccgga
     2041 ggatgaaatg gcggcgaaca agatggttca ttatttgcag gagagaaaac tggaagtgga
     2101 ggagttgaga gactatgtgg gataatgcaa cgattatgat gctggtggaa ggcacctttg
     2161 ttactttgta tatgaccctt gtttccacat ttatggggta tgtactcgga cttcctatgg
     2221 gaattgccct ggtgatcact gcaccgaagg gactgcgtcc gaacaagatc atttataaga
     2281 ttctggatgt gatagtcaac attgtgcgaa gcattccgtt tttgattttg ttgatcatga
     2341 tcatcccttt gacccgtatc attgtaggaa agagctacgg tccgacggct accatcgttc
     2401 ctctgactct ggcagcggca ccgttcattg ccagaatggt ggaatcttct ctgttggagg
     2461 tagatcacgg agtgattgaa gcagcacaga gcatgggagc taacctgtgg acgatcatct
     2521 ggaaggttat gctggcagaa tccagaacct cattgcttgt gaatgcgacc attgcactgg
     2581 gaacaatctt aggctactcc gctatggcag gtgccgtagg tggaggcgga ctcggtgata
     2641 ttgcgatccg atacggttat tatcgttacc agaccgatat tatgctggtg acagtagtat
     2701 tgttggtact gttggtacag ctgctgcaat atatcggaat gacgatttcc aagaaattgg
     2761 ataagagaac ccgttaggga aaacgaaaag attgaggcag atgtaaggca tctgattcaa
     2821 tagactaaaa ttttttacca aaagatttag gaggagaatt atgaaaaaga aagtgattgc
     2881 tttgattgca gttgcagcat tgtctgtgag cgcactgacc ggctgcggca gcaacgaaac
     2941 agctgcgcag accagcgagg cagctgcaac aacagctaac caggaaaccg ctgcccagac
     3001 tactgaggca acagaaagca aggaagtagc tgacactgat accactatca aggtagctgc
     3061 aagtgcagta cctcatgcag agatcctgga ggcagcaaag cctctgttag cggagcaggg
     3121 ttatactctg gagattcaga tttttgatga ctatgtacag cctaatttag tagtagagtc
     3181 cggtgatttc gatgcaaact acttccagca tattccttat ctggagagct tcaacgagga
     3241 gaaaggtacc catctggtaa atgccggcgg tatccattac gagcccttcg ggctgtatcc
     3301 cggaactgag accagcttag acaacattga caatgcaact atcgcagtac ccaacgatac
     3361 caccaatgag gcacgtgctc tgttactgtt acaggataac ggttacatca ccctgaagga
     3421 tggtgtaggt ctgaccgcta ctaccaagga tattgtagag aatcctcaca acatcacctt
     3481 cgtagagctg gaggcagcac aggttccaag aacccttccc gaggtatcct tcggtgtcct
     3541 gaatggtaac tacgctatgg aagcaggcct gactgttgca gatgatgctc tgttattcga
     3601 gagtgctgat tctgaggcag cagctactta tgtaaacgtt atcgctgtaa aggaaggcaa
     3661 cgagaatctg cctaagatca aggcactggt agatactctg aagtctgatg agatcaagca
     3721 gtatatcaat gacaattata atggtggcgt tatcccttac aagtaagatg aagcgacagt
     3781 aaagtaatgc gaaatccgga gagcattcca tcaagtagca ttcatgggtg ctctcctgat
     3841 ttttctgttg tttccacaaa tcctatatga actctgggaa aagagaaaag aagatagaat
     3901 ctgtggaaac cgtggaaaat ttggagcagt tcagaatcgc tttggtataa gccggcggac
     3961 attaaaagtg aaacagagca ggaagatgct cggaaatgag gataggatga aaatttcaac
     4021 caaaggaaga tatgcagtac gtgtcatgct ggatctggca ttgaacaaca acggagagtg
     4081 tatcaaggta aaagaaattg ctgccagaca ggggatttcc gaaaagtatc tggagcagat
     4141 cattgctgtt ttgaacaagg caggttatgt aaaaagcgtc cgtggtgccc agggtggtta
     4201 tcgtattgcc aaggatccgg cagattttac tgtaggaatg atcttacgac tgacggaagg
     4261 ctcactggct ccggtggcat gtctggagga aggcgctgat atctgcgaac gatgtgatac
     4321 ctgtgagact ctggaggtat ggcaggaatt gtatgatgca gtgaacaagg tagtggacgg
     4381 agtcaccatt gcggatctgg tggaacgacg gaacaagcgc ctggagaatc tggattactc
     4441 catataaaga taggaataat agaagacaat aacgcagccc gacaggagat cctgccgggc
     4501 tgttttttgg gtttgcagag aatgaaaggg ggtcattcct gcagaaaatg ttaactgggg
     4561 gaatgaatgg ttaacaactc aagtatatcg agagaactgc ccattgtcaa cgaatgttgt
     4621 atgttggttg acaaactttc atcgacaaat atcgcgtaat tcggattttc catacatata
     4681 gtaataaaaa ctacataaaa caacataaaa caccacgaaa tgaaatataa taacaaaaac
     4741 tcattgcttt ttactccaca acgtggtaag ctaaactctg caaacaagta gaaaggtgta
     4801 tgcgataatg agcgaagcat ttcatattat atcagacggt tcctgtgatc ttcccatgga
     4861 gctgaccaaa gaaaaagaca ttacggtagt tcccttttat gtatcttttg aagaggggaa
     4921 ataccagaag gaaatgatag agatcggtgt ccgtgagttc tatcaggaaa tggtggatca
     4981 cccggatgta tatccgaagt cttccatgcc ttccgtgcag gattatgtag atgcatttct
     5041 tccttttgtc aaggcgggaa ctcccatcat ctgcatctgt atcaccacca agttcagcgg
     5101 ctccatgcag tctgctctga atgcaaaagc gatcctggaa gaggaatatc ccaatgcaca
     5161 gatttatgtc tgcgactcta ctgtgaatac cgtattgcag ggcatctatg tattggaagc
     5221 ggtacgcctt agagatgcgg ggaagagtta ccaggagagt atcgatcgcc tgaatgagat
     5281 caagatctcc ggcagaatct ttttcaccgt aggaaatatg gaatatttaa agcacggcgg
     5341 acgtatcggc aaagtagcca gcgtggccgg cagcctgctt ggcatcaagc ccatcattac
     5401 cttaaaagag ggagaaatct tcccctccgg tatcggcaga agccgtcgca agacggtaga
     5461 taaaaatatc gatttgctgt tagcatattt tgcagaggaa aaggcagata ttgccaatta
     5521 cagcgtctgc gtgggatatg gctacgatta tgaagaagcc gtagagttcc gcgacagact
     5581 ggcaggcaga ctgaaagaaa agggatacga catcgacacc attcccatca tccagatcgg
     5641 tgccaccatt ggcgtacaca ccggacctta tccgttggga atcggtgtcg tgaagagagc
     5701 tatttaaaat ttatttcacc ctggcaggca tttaataatt cctgcacatt ttcaaaagca
     5761 tagtccggca gatattgggt attcggctcc ccctcgggag tcagaatatc ctttttttgt
     5821 gcctcgccgg tgaagagaac acaggtgtcc acaccaccgt tgatgccgca ggcaatatcg
     5881 gtgtacagac gatcgccaac caccagagtc tcctcgggag tgaagccgga gacttcaagg
     5941 caaagcttta cgacctcttt gctgggcttg cccagataga tgggctgttt gtctgtggtg
     6001 gcggtgatca tgtcgcagat ggcaccgcaa tcggggataa agccgaagtc gatggggcag
     6061 cgcagatccg ggttggtgcc gtagaaagga acatcctggg tgaataaaat cttgcagact
     6121 tcggtaagct ttgcataatt taattcactg tcataggcaa cgaccacgca gtcaatgccg
     6181 tcctcgcaga cctcggttac ccagaggcca ttctttcgca gttccgccac aaaagaagcg
     6241 gttcccagca cgaagatttt cttgtctgta taattctcct tcaaaaaacg cagcgtcata
     6301 tagccggagg tgatgaactg atcctccgtg ctgtccaggt gaaatgcatc gtggaatttt
     6361 ttcacatagt ccagtccgct ttttgtggaa ttgttggtaa tgtaataagc cctgccgccg
     6421 atggcctgaa tgtagcggag aagatccgca cttccctcat acaaggtatc gcctacggca
     6481 atggtgccgt cgatatcgaa caggaaaagc tttttatctt tcagacgagc cagctttgcg
     6541 gcatcgctgt aaatgtgctt cgggtcaaaa gtatccataa ttatcctcat ttctaagcat
     6601 caaacgctca ggttcatgtt agcaacgaat atccccactg tcaaggaaaa gacggcttct
     6661 ggagggaaac gtgagtggaa ttgcggaggc atttatggta tgatgggcat aggcatgagc
     6721 tgtgttgcct ggggctctta tggaattcga cagattgcat acaagattgc gcaagggaca
     6781 tgggaaatag gatgaagtga tttaacgact acaattaaaa aggacaacat tacggttatg
     6841 gacgaaaaga aagataaagg tttacaggga aatgagaaaa tagaggaggc aattctggca
     6901 ttacagcagg agccgacaga agaacttctg gcacatgctc tgacggtggt gcgcagacgt
     6961 atgcgggagc agggacagct tatcgtggcg gtggaaccgc cctcagcaga tgtgcaggaa
     7021 gcggtgacac aggccgccgg aactgccatg cgcctgcagg cggtgcagac ctccgatggg
     7081 aaaaaatggt ggtcggcctt caccggattt gaggaagaat taaagggaag cggcagcgta
     7141 atgtccactt ttctcacgga tatggagcag ctgttccgca tgacgttgac tgctgatggt
     7201 atcgagggtg tgatcctgaa tccctggaac cggacgttga tgctggacaa gcggttcctg
     7261 catattattc tcggagaata gcaaaagagg gagaagcgga gcatgacaga gagctatgaa
     7321 ctgcccgtgc tttttacgga cgatgaatgc aaggcttatg ccgcatgggg catgaaaaaa
     7381 gaaaaagtac tgatgccgtt tgccctgatc actatactga tctatatggt agcaaccgtg
     7441 ctggtagtga tgaaggtctg gggtgtgcgg gagagtgacc atctgctgtt ccagatgctg
     7501 ttgtggggtg actggattcc ggaactgacg ggagcgctgg cggttttcat gacgatcctg
     7561 ttactgggac cgttgaactt tatactggat aaattatgga aaaaaccggc agagcccatg
     7621 tggctgtgtg tagtaccgga gggtggaact gtgaaggtca ctgccgtgag aggtctggaa
     7681 ggtaatcccc ggacaccggc agagatctat ccactgacgg aactggaaac ctttttaaac
     7741 ccggcggaca acaccatctg ctatcagaag aaatggtatc ggatcgggga aaataccata
     7801 gaaaatattt atccgccgga aaaacagcat ccatggatgg atcacccgga aaataaagcc
     7861 agcggcatca tcgatgtaca gagacttatg gagatcctga aagggtacga agcgtctctg
     7921 aaggctgccc ggaaggaatg tgaatggatg gaaaagcacg gcggatagaa cgaatagatg
     7981 acagaaaagc ggataaaaat tatgaccggg ataagatggc ataagacaaa tcaggaaaga
     8041 ggtagggaaa atatgaaact gatctcatgg aatgtgaacg ggctgagagc ctgcgtgcag
     8101 aagggatttc tggatttctt tcaggaaacg gatgcagata ttttctgctt acaggagaca
     8161 aaattgcagg agggacagat cgcactggat ctgcccggat atcatcagta ttggaattac
     8221 gcagagaaaa agggctattc cggcactgca atttttacaa aggaagagcc catacaggtg
     8281 acttacggca tcggcgtgga ggaacacgac catgagggcc gggtgatcac tgcagagtat
     8341 tcggagttct attttgtgac agtctatgta ccgaacgccc agagagaact gacgagactg
     8401 gaataccgca tgcagtggga agcggatttc ctggcgtatc ttaagaagct ggaagagaaa
     8461 aagccggtga tctatgcggg agacctgaac gtggcgcatc aggaaatcga cctgaaaaat
     8521 gcgaagacca accgtggcaa tgcgggcttc actgatgaag agagagcctg cttttcgaag
     8581 gtactggaga acggatttat cgatacgttc cgctattttt atccggacct gaccggggtg
     8641 tattcctggt ggtcttatat gttccaggcg agagcaaaaa atgccggatg gagaattgat
     8701 tatttcgtgg tatcggagta tctgaaagac cggctggcgg atgcgaagat ccacacggaa
     8761 attttgggat cggatcattg tcccattgaa ctggatctga aataaacgga cagcataacg
     8821 gaggagactt atgagcttac gtttctgttt tggcccttcc gggtcgggca aaagccacag
     8881 aatctatgaa gagatcatgc agcgggcggc agaggaaccg ggcaggaatt tcctgatcat
     8941 tgtgccggat cagttcacca tgcagaccca gaaggatctg gtcatgcgca gtgaccggga
     9001 tggcatcctg aatatcgatg tcctgagctt cggccgactc agccaccgca ttctggagga
     9061 agtggggacc aaagagatgc cggtactgga tgacaccggc aaaagcctgg tgctgcaaaa
     9121 agtggcggcg gatctgaagg aacagcttcc ggccatggga agtctcctgc acaagcaggg
     9181 ctatatccat gaggtgaaaa gtgcgatctc cgagttcatg caatacggta tcagcacaca
     9241 ggatatggac aagctgatca ccagtgcaca gaagcgtggc gcgctggcca tgaaactgaa
     9301 agacttaaag actctgtacc ggggctttca ggattatatc agggatcatt ttatcaccac
     9361 cgaggagaca ctggatgtac tgcgcagatc cctgtccaaa tcaaagatct taaagggcag
     9421 tgtagtggtg tttgacggct ttacgggatt tactcccata cagaaccggc tgatccagga
     9481 actgatgcgc gtgtgcgcag agaccatcgt taccgtgacc attggagtgg gagaggatcc
     9541 gtataaaatg gatggtgaac agaagctgtt ccatctgagc aagaagacgg tggcggatct
     9601 ggaaaagctg gcagcggagg cagaggtaga gcggggagag gatctctttg tcaagggggg
     9661 gcccaatcgt ttcgccaagg caccggcatt acactatctg gaacagaatc tgttccggta
     9721 tcagtatgaa ccgtatgcgg gagaacagca agagatccat atgtttgaag ccctttcccc
     9781 cagagaggaa gtccaccaga cagcgctgta catccggcac ctgatcagag agcaggggat
     9841 gacctaccgg gacattgccg tggtcatcgg agatctggaa ggatacgctt cctatgtgga
     9901 aacagaattc ggacagctgg agatcccatg tttcctggac cgtaccagag ggatcgtgct
     9961 gaatcccatg atcgaatata tcaagagcgc attgcagctg tatattaagg atttttccta
    10021 tgatacggtg tttcattttc tgcgcagcgg catggcggat atttccagag aagagattga
    10081 tgagctggaa aactatgtga tccgcaccgg ggcgagaggc tatcggacct atagccgtct
    10141 gtttacccgc agaacggaag aactgcaggg aaatgcggag gggagcgaac aggcggaaga
    10201 gaagaccatg gagcgtctga accgtatccg gcaacagttc atggatgcgg tggagattct
    10261 gcacatggga agccaggaaa aggctggaga ctatgtcagc catctgtacg attttctgga
    10321 acagaatcag gtgcagcaaa agcttttgaa ctatcagcag cagtttgaaa aagagggaga
    10381 cttatccaga gccagagagt atgcacagat ttaccggctg gtcatggatc tgctggatca
    10441 ggtctatgaa ctgttgggag aggaagaaat ctccagacag gaatttgcgg atattttaga
    10501 ggccggtttc ggcgagatca ctgtcggtac gatccctcag aacgtggatc gtatcgtggt
    10561 gggcgatatg gagcgaaccc gtctgaaaca ggtgaaggta ttgtttttcc tgggagtcaa
    10621 tgatggaaat attccgaaaa atgcttccaa gggcgggatc atttcggata tggatcggga
    10681 attcctgatt gaatccggga cagagatggc acccagcccg agacagcaga tgtatatcca
    10741 gagattatat ctgtatctga atatgaccaa gccctcagaa cagctgtatc tgtcctatgc
    10801 caaggtaaac agtgaaggaa agggcataag accgtcctac ctgatcgata cggtgcggaa
    10861 gctgttcccg gcaatgagcg tggagtatcc ccagaaccgc agccgtctgg aacagatcga
    10921 gggcagacag gaaggtgcca gatatctggc ggaggaactg cgggaatatg tggaaggaac
    10981 cctgccggaa gaagagaggc aggacttcta tctcatgtac cgtgcctacg aggcggatgc
    11041 ggcgggcaga gacctgctga cccgggcggc atttcgcaga tacagggaga gcggtctgtc
    11101 ccggatcgtg gccagagcat tgtacggaca gcagctggaa aacagtgtca gccgtctgga
    11161 aacttatgct gcctgtacct gcagacactt tttgcaatat ggtctttccc tgcaggaacg
    11221 ggaagaattc ggcttcgagg cttcggatat gggtacggta tatcatgcag tactggaaaa
    11281 ctttgcagga aaacttgcag aatccaacct gacatggtgg gattttacgg aggattttgc
    11341 agccaaagcc gtcaaagaat ccgtggaagc ctatgcggca acctacgggg agacggtatt
    11401 atacagctct gcgcggaatg aatatgccat tacccgcatg agcaggatcc tgacccgcac
    11461 agtgctgacc ttgcagaaac atttgaaaca gggcagtttc cagccagatg attatgaact
    11521 ttctttccgg tttgcggagg atctggacag cattcatgtg gatctgtctg aggacgagaa
    11581 gatgcatttg cagggacgga tcgatcgtat cgatgtgtcg gaagatgcag agcatgtcta
    11641 cgtcaaggtc attgattata agtccggcaa ccggaaattt gatctggcgg cattgtatta
    11701 cggtctgcag ttgcagctgg tggtatatat gaatgcagcc atggagatgg agagccgaaa
    11761 gcatccggac aaggagatcg ttccggcggc acttctgtat tatcacatcg atgatcccac
    11821 catagagaca ccggtggagc tcacggatga gcagatcaat gaacagatcc ttgcaaagct
    11881 tcgtatgaac ggtgtagtaa acagtgatcc tgaggtcgtg gagcggctgg accgctatat
    11941 gcaggataag tctgtggtca ttccggtgga gaagaaaaag gatggaagct tcagtgccag
    12001 atccggtgtc ttaagccggg aggagatgca gctgatatct tcttatgtag atgccaagat
    12061 ccgcagcatc ggacgggaga tcctggacgg aaaaatagca gccaatccct atgagaaagg
    12121 caatgaagag gcttgtactt attgcgccta taaaaaggtc tgcggtttcg atggcagtat
    12181 tccgggctat gagaagagac agctggaaga tctggataag caggcgctga tgcagcggat
    12241 gcagaagacc gtggaagctt agggcaaagg gtgcccggcg ggagtgtgtg cctgacagga
    12301 agctcgaaga gaaagcaccc gctgcccgga ggaacaggac ggcaacagga gaaaagaaac
    12361 aggaacagag gtagcgtatg ggaatgaatt ttaccccgga acagcaaaag gtaatcgaac
    12421 tgcataacag caacattctg gtatcggcgg ccgcaggaag cggtaagacg gcggtactgg
    12481 tagagcgtat catccgtatg atctgtgagg gggagcatcc ggcggacatt gaccgcctgt
    12541 tgatcgtgac tttcaccaat gcggcggctg cggagatgcg ggagcgtatc gcagccggga
    12601 tcgccgccag actggaagcg gatccgggga acgaccatat ccagaagcag tccgccctgt
    12661 tacacaatgc ccagatcacc acgatcgaca gcttcagcct gttcctgatc cggaatcatt
    12721 ttaatgagat cggtctggat ccggatttcc gtgtggcaga cgagggtgag atcaagctgt
    12781 tgcagcagga ggtgctggga cagctgctgg aggatgccta tgcggggaaa tttgttccgg
    12841 atgatgttcc gatgtcccgg gaaaaccgtg acagcgagga cccatcccgg gaggcacggg
    12901 aacgtttcca cgcctgtgtg gaatatttct gccccggcgg cagagagagc gtgctggaac
    12961 agcatatcct gaacctgtcc cgctatgccg gaagttttcc ctggcccgca gaatggctgg
    13021 aagagcgtaa aaacgattat gcggcgggag atctggatgc cctgctgcac agtgattacg
    13081 gacaatatct ggcagagcgt gtgagccgtg tcctgcaagg gtgtctggaa aaactggtgg
    13141 aagtgaaaaa gctgtgtgag ctgccggacg gtccatatat gtatggagaa ctgacagaag
    13201 cggagagcga acagctggaa cgcctagcgg cctgcaagga tttgaaggaa caggcagcaa
    13261 aggttccagc agtgactttc ggccgtcttt cttccaagaa ggatgagagc gtggatcccg
    13321 ccaaacggga gctggcgaaa tccatccgta atagcgtaaa agacacgctg gcggatctga
    13381 cggaacaata tttcaagaca ccgttagagt tggtggtgga acagggaaaa gcctgcagag
    13441 agccgctccg gatgctgctt aacctggtgc tggaattcga cagaaggctt cttgcggcca
    13501 aacaggagcg acatctgatc gacttttccg acatggagca ctatgcgtta cagattctgc
    13561 taaaacggga aaaagtggaa gagaccggcg atgcagggac ggacagcacc ggtgatacag
    13621 gaatggatag caccggtgtg aaatatgaca ttgtgccaag tgacgtggca ttggagtatc
    13681 gccagtattt tcaggagatc ctcattgacg aataccagga cagtaatctg gtacaggaat
    13741 atctgttaag tgccatctcc ggggaagcgg agggacatta taaccgcttc atggtaggtg
    13801 atgtgaagca gagtatttat aaattccgtc tggccagacc ggaacttttc ctggaaaaat
    13861 acgacaccta tcaggagagt ggggatctgt gccggatcga cctggcgaag aacttccgaa
    13921 gccgtgtgca ggtggtggat gcggtaaacg atgtgttttc ccgcatcatg agccgggaga
    13981 tcggcggcat tgcctatgat gataaggcag cattgtatcc aggggccaca tatcctgcca
    14041 cagaggatcc tgcatacggc agtgaactat tactgatccg taagccggag aagggggatg
    14101 gtgcggaagg cggcagcgga gaacagcgtc ccgacggggc aagaggtccc gtggactatg
    14161 acaacgtaag acagctggaa gcactggcta ttgcggcacg tatcaagcag ctgaagggca
    14221 gtctgaaagt catggagaag gctacgggag aattgcgccc ggtgcggtac tcggatatgg
    14281 tgattttgct tcgtaccacc agtggctggg atgaggaatt caagaaagta ctggaacagc
    14341 agggaatccc tgtctacatt acatccaaga ccggatattt cggggccctg gaagtacagg
    14401 aactgctgca gtttttacga gtgctggaca acccgagaca ggatattccg ctgttcggcg
    14461 tgatgcagtc gatcttcgga ggctttgccc aggaagaaat cgcccggatc cgcagcggaa
    14521 gcaaagggca ttcccggaag agaatgacac tgtatgaggc attgaaagaa gtggcgcaga
    14581 ggggacggat ggcggaagcg ggagaagagg catcagcggg cgaaagcgcc ggagaagaaa
    14641 ccgccggggc cggacagtac tggcagcgcg cattggaagc tgcaacagta ccggaggaca
    14701 tggaaccgga gacagaactt tcccaaaagg cagatacttt cttacaacgt attgatcatt
    14761 accgtgatct gactcccttc acctccatcc gcgacctgct gcagcggatc ctggacgatt
    14821 acgattatct gaactatgtg acggcacttc ctgctggcag caagcgccgg gccaacgtgg
    14881 agatgctgct gacgaaggct tctgcttttg agaagaccag ttattttgga ctgttccatt
    14941 tcatccgtta tatggaacag ttggaaaaat acgatgtgga ttacggtgag gcggataccc
    15001 tggacgaaaa tgccgatgtg gtgcggatca tgagcatcca caagagtaaa ggtctggaat
    15061 tccccgtggt atttgtctcc gggctgtcga agcgtttcaa catgcaggat gcgaaccagt
    15121 ccctgatcgt ggatatggat ctgggcgtag cggtggacta tgtggattcc ggccgccgca
    15181 ttaaaaataa gaccctccga cgggccgttc tgtcagcgaa aatgaaggag gacaatctgg
    15241 cggaggaact gcgtgtgctt tacgtggcac tgacccgtgc cagagaaaaa ctgatcctga
    15301 cagcggtgtt ggataaggcg gaggaaaaat gggagctgtc ccagatgaca ggacaggaga
    15361 agctgaccta tctggatttc tgtgaggcgg ggagctatat ggattttctg ctgccgatcc
    15421 tgcctaagac aggaattgca gtaacaacgc tgggaacaga agacctggcg gcagaggaga
    15481 tcagggaaca actgcgcatg ggagaccgca gggaactgtt gcagcgtgtg acggacggcg
    15541 agactccttt gcctggggac ccggaggaga acgaacgaaa gcttgcgaaa ctgcgggagc
    15601 gatttgccta tgcctatccg tatcccggcc tgcagaagct ctataccaag accacggtat
    15661 cggaactgaa gattgcggcc atggcggaga aggatgaagc ggcatttcat accttcgaag
    15721 aaaaagaagt ggtgccttat atcccggctt tccgcagaga acaggaaaag gtcagcggag
    15781 ccgtccgcgg taacgcgttc caccggacca tggaactgtt ggacttttct tatctgttta
    15841 tggaatccgg actatttgcc ggatgccctg ggacttatga ggaataccgt cagggcctgg
    15901 atacggaccg gttacaggaa cgtctgaagg aattcctgca gcgggagaca gcttccctac
    15961 gactgacaga agagtatgcg caggccgtaa gccttcccaa aatccggcat tttctggaac
    16021 aggagctggc ctaccgcatg tggagagctt ttgaacaggg ccttttgtac agagagcagc
    16081 ccttcgtgct gggcattgat gcgaagcgcc tggatcagga ccttcctgaa ggggaaaagg
    16141 ttctcattca gggtatcatc gatgtcttct ttatagaaaa cggagagatc gtcctgctgg
    16201 actacaagac cgatgtgatc gattccctgc aagccctctg gaaccgctac agcgtccaga
    16261 tccaatacta tgaggaggca ctgacgaagc tgatgcagct gcccgtgaag gagaggatcc
    16321 tgtattcctt ctatctggaa aaatacgagt aaaaaagccg gagtaaatgg aggtttcata
    16381 tgagtcaata ttttgattta tcaataagaa ggattgagat aaagaaacca aaaagagact
    16441 tgaaacctgt tattaaatta caggatgaag ttaataagac tttgctgcat gatgaatatt
    16501 attatcctac agatgaaaga actatacagg aatgtttgga tggtgattat atcttatt
//
LOCUS       DAWEJW010000062        16544 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1304_length_16544_cov_61.294135, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000062 DAWEJW010000000
VERSION     DAWEJW010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16544)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16544)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16544
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1304_length_16544_cov_61.294135"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5141 a         2941 c         3949 g         4513 t
ORIGIN      
        1 gagcagagga ctgaaaatcc tcgtgtcact ggttcgattc cggttctggg catttgtaat
       61 atgcaaaaat aaatatttat gggatactag ctcagtcggt agagcacttg acttttaatc
      121 aagttgtcgg gggttcgaat cccccgtgtc tcattgcaga gagatcaata tacggtttgt
      181 atattgatct cttttaattt acgcgagcaa cgagccaaag tccgtgcttg cacgagatct
      241 tggcgactgc cgcgataact tgagaaactc gcgaagcgtg tttctcatag ttttcccagg
      301 cataagagaa cctatatcag accattgcct gcacaagcat gatatgctaa aataattcgt
      361 cggaaacaaa gaaatcaacg aaagagattt aaaatcgaaa tggaaagtaa agtaaaagaa
      421 ttgcgttacg caatcgtaca gtccatacca gtcatgctgg gatatctttt cctgggattt
      481 gcctttggac tgatgctgaa tgatgcagga tacggcttct ggtgggcctt ttttatcagc
      541 ttgtttgtgt atgcgggaag catgcagttt gtgttggtca ccttgctgac ggcgggagca
      601 agtctgtggt atacactgat catgacgttg tttgtcaatg gcagacacat attttacgga
      661 ctttcttttg tagaaaattt caaaaaaacg ggtataacat atccttatat gatcttttcc
      721 cttacggatg agacgttttc tctgttatgt gatctgaaag ttcctgaggg aatgcgtgag
      781 gaacgagtca gcttctacat ttctctgttg gatcattgct attggattct gggaagctgt
      841 gtaggtgaac tggccggaag ggtcctgcca atcgacacta cgggaattga cttttccatg
      901 acggcattat tcacagttat agtagtcaac cagtggatgg atacgaaaga acataaaccg
      961 gcagtcatcg gaggcgttgt cggagttctg tgtttactgg tactgggcgc agacaccttt
     1021 ttattacctg cattgaccat tgccgcaatc atattattag gagttaagaa aaaatgttac
     1081 attccgtcgc aatcatagca gttgcagcta ttgttacttt gatcatacgg gcagttcctt
     1141 tcgtggcatt tggtggaaag agagaggttc cggaaacagt cacatatctg ggaaaagtac
     1201 tcccgccggc cattatggtg attcttgtta tttactgtgt aaaaggaatt aatctgttta
     1261 ccggcagtca tggatttccg gaattgctgt ctattgccgt ggtggcattg cttcatatct
     1321 ggaagaaaaa tacgttgctg agtatagctg tagggaccat actttatatg atattagtgc
     1381 aagtagtatt ctaaaacagt gtgaaaaggc tttttttcag ccttttcaca ctgttttttt
     1441 acgaaattaa atgctataat gagcgcaaca gaaataagat gagggagaaa cagaagataa
     1501 aatggaaacc gataacaaag aattaaaaga agaaacaaca aagaaagagg ctgataagaa
     1561 agaaacggag agcaaagagg aaacaaaaca gcctgtggaa gagacagcgg tacagaagaa
     1621 aaagagcaaa aaatggattg gtattgtagc agctgtagtt gcagtaattc tattggcata
     1681 tggtggtgtg gcaatttact accagtctca tttcctgaat catacttata tcaataacag
     1741 tgattgcagt aatatgacgg cgacagaggt agcggcgatc atggatcagc agtcacagca
     1801 gtatagttta cagattctgg gcagagatga gaacggtgtg caggaagaga tcggtacgat
     1861 cacagcatct gagatcggaa tgtactgggt ggatactttg aatgcggcac aggagctttt
     1921 ggacagacag aatgaattcc tgtggattga gatgctgtgg agtacgcaga atcatgatgt
     1981 agttcagggt gtgtcctatg atgcagacaa attgcaggaa caactggcac agatgcctgc
     2041 actgcagaac aaaaatatga ttgcaccgga agatgcttat atcagtgaat attccgagaa
     2101 gaacaagaat tatgaaatca ttccggagac catgggaata gagttgaaca acaacctggt
     2161 agaagaagta gtatccacag caatcatgca gggagatacc acggtagatc tggaggagca
     2221 gggctgttat gaaactgcca aaataacggc agaggatgca gcgctggtaa aagcctgtga
     2281 taccatgaat aaatgggtca gcgcacagat cacctatgac tggaacggca ataaggttgt
     2341 cgtagacgga gatacgatcc atgagtggat ccagacggat aacaaggatc cccaactgga
     2401 cgaggaagca atcgaggaat tcgtagcaga gcaggccaaa gaatacgata cctatggcaa
     2461 aaagagaaag tttaccacag ttcagggaat cgagcttacc ttgcccagcg gagcttacgg
     2521 ctggaagacc gaccgcgagg aagaggtcaa agaactgaca gcttctattg aaaagggaga
     2581 aaacatcgac aaggaaccgg tatacagcag caagggagca caaaagggaa gtgacgatat
     2641 cggtaattcc tacgtggaaa ttgatctgac gaatcagcat ctttatctgt tccagaatgg
     2701 aagcattgta ttggagacag attttgtatc cggtaatatg agcaaagcgg gctgcatgac
     2761 ccctcccgga gtgttcggac tgacctataa gacaaaaaat gctgtattac gcggtgcgga
     2821 ttatgagacg ccggtaaact actggatgcc ttttaacgga aatgtcggaa tgcatgatgc
     2881 tacctggaga ggaagcttcg gtggaaccat ctatctgaca tcaggctctc acggttgtgt
     2941 caatctgcca ctgaatatgg cggcagctat ttatgaatat gtatctaccg gtttcccggt
     3001 agtatgctat tattattaat gcgaatcagt gtggctatag acgaggggat attgagaagg
     3061 aaggcagttt tatgaaacag cgttcagcaa aaaaagtgca gaagtggaaa gagagatttg
     3121 aaagagaacg acagttaagg caggagatcc gggcggtagc accggatatt ctgcgttcta
     3181 agaactttcg ctccacacgg tcacatgtac agcacggtaa catgacagtc aatgatcaca
     3241 gtatgaatgt ggcaaaatat agtcttgcca tcagcgagaa gctgcatatt ccctgtgaaa
     3301 aaaaggattt gatccgtggg gcattattgc atgattactt tctgtacgac tggcacacgc
     3361 cggatcatat cagcccccat aagcttcatg gcttttatca tccgggaaga gcgcttcaca
     3421 atgcacagaa ggaataccat ctcactccca gggaaaaaga tattataaaa aaacatatgt
     3481 ggccgctaac aatctccgca gttcccatgt gtcgtgaagc ctggatcgtc accatggcag
     3541 acaaatggtg ttcgcttatg gagacatttc atgtacataa gggacatgga gccctgatcg
     3601 agaagcttcg gaagattgaa gaggagcagg gaaaataaaa gggtggaaac ccaaaacagg
     3661 agaatgacgg aattgggtga attataccgg agattaaaag actacagtaa gacagattat
     3721 tatccttatc atatgccggg acataagaga ctgctttgtg gagaattccc ccgggagatc
     3781 atggatatcg acatcacgga gattgatgga tttgataatc tacatgctcc agaggaaatc
     3841 ctaagacgat tacagaacga ggcagccgga ctgtacggcg cagacgagag cttttacctt
     3901 atcaacggaa gtacctgcgg ggttctggcg gcaatcagtg cggcggttcc gaggggcggg
     3961 cggatcctta tgaccagagg agcccataaa tctgcgtatc atgcggcata tctgagaaat
     4021 ttaacagttt cttatctgta tccggaaatg atggaggagt atgatattta tgatgcggta
     4081 actccggaac agatcgcaga ggcattcagc cgggagcctg ttccggatgc ggtatttctg
     4141 gtgtcaccca cttatgaagg caggatcgca gatatagaga cgatcgcaaa gctggtacat
     4201 agtaaaggaa ttcctctgat cgtggatgaa gcccatggtg ctcatctggg actggcggaa
     4261 ggatttgcga agaacagctg ccagtgtggc gcggatctgg tgatccacag tgttcataag
     4321 actttgccgg cactgacaca gagcgcgttg cttcatgtca acggaagact ggtcgacaga
     4381 gaacgtttgc gcaggttttt gcatatttat cagtccagta gtccatccta tgtactaatg
     4441 gcaggaattg acaatgcact acagctggta aaggagcagg gagattatct gtttgcccgg
     4501 ttgcaggtga attatctgag aatgctgaca gaactgtcag aatgcaggaa ccttcatttc
     4561 ttgccgctgg atgccagaca ggatatcgga aaactggtga tattatcttc aaaggcaggt
     4621 ttgtcaggac agcagattta tgatattcta ttagaggagt accacttgca gttggaaatg
     4681 gcagctgcag atcattgtct tgcgatgttc accataggag acagtgacga ggcatacaca
     4741 agaatgacgg atgcattgct tgccatcgat cgtgacatat ctgcgggaaa gtggcagacg
     4801 cagccacaga cggaaggcgg agattccaga gagacatctc tctatcacat gcagccggaa
     4861 gtcagtttgt ctcttgcgga tgcatgggat gccgaaaaag aggagattcc tctggcacag
     4921 gctgcaggca ggattgcagg agattttgtg aatctgtatc ctcccggaat tccggtgctg
     4981 gtaccgggag aacagatcga agagatccat tgccgaaagc ttgcggagta tttcagaaag
     5041 ggtctgaagg tacagggagt caccgaaaaa gacgaatatt gcattaccgt agtcaaaaat
     5101 caaaaaaaat agcagcagaa aggatttagc ctgtatgaga aaaaccaaaa ttatttgcac
     5161 catcggtccg gccagtgaga gtgaggagaa attaagagaa ttgatgcttg caggcatgaa
     5221 cgtggcacgt tttaactttt cccatggaag ccataaggaa cagctggtta aatacaacag
     5281 agtactgaaa gtacgaaaag aactgggatt gccggtagca actctgttgg ataccaaagg
     5341 accggagatt cgtctgcgtg attttgaggg cggtaaggta gagctggtat ccggtcagca
     5401 gtttgtcctg acaacggaag aaatcatggg aacagcggaa agagccacta tttcctacaa
     5461 aaatctgaaa aatgacatca atgtcgggaa aacgatcctg atcgatgacg gtctgatcga
     5521 aatgacggta gaagagatca aaggagaaga cattgtatgt aaggtcataa acggcggttt
     5581 tgtatcgaat cataagggtg tcaatgtacc gggagcgatt ctgtccatgc cttatatcag
     5641 tgaggtggat ctggcagata ttgtgtttgg tgtagagcac ggatttgact ttatcgcagc
     5701 ttcctttgtc agaaccaggg aagatattca ggaagtgcgc aagatcgtag aggacagagg
     5761 cagcaagatc aagatcatcg ccaagatcga gaacatgcag ggaatccaga atctggaaga
     5821 gatcattact gcagcagacg gtatcatggt agcacgtggt gacatgggtg tcgaaattcc
     5881 tctggaagaa gtacctattc tgcagaagaa gatgatcaag atggcagtgg cacagggcaa
     5941 gcatgtaatc actgcgaccc agatgctgga gtccatgatc aagaatccac gtccgaccag
     6001 agcggaggca acagatattg ccaatgcgat ctatgacgga accacagcca tcatgctttc
     6061 cggcgagagc gcagccggtc tgtatccggt agaggctgtt aagaccatgt cccgcattgc
     6121 ggagagagca gagcaggata tcgattatcg cggaagaatg cagagagtca aggaagacag
     6181 acaggaaact ccggatatta ccacggcaat ctcctatgca acctgcagtg tggcttccga
     6241 tctgaatgca gcagccatca tcaccgtaac catgtccgga tttaccgcga atatgatcgc
     6301 acgttataag cccggatgcc agatcatcgg ctgtacattg gatgagaagg tataccgcca
     6361 gttaaatctg ctgtggggtg taaaacctgt catgatccag aaggagcgga ccacggatgc
     6421 tctgttcgag gaagcggtat tcaaggcaaa gcaggcaggt ctggtgaaaa ccggggatac
     6481 ggttgtcatc accgcaggcg tacctctggg cgtggtaggt aagaccgata tgatccacgt
     6541 ggtggaagta gaataaaaac gtactgtacg gcggtcaaac agccgaaaca ggagcgggaa
     6601 aacgagaacg cagatatggg aaaaattgta tatctcatgg ggaaaagttc ctccggaaaa
     6661 gacaccatat ttaaagaact gatgaaagag ggcacgatgg atcttcggac catcgtgcct
     6721 tatacgacaa gaccgatccg ggccggggaa gagaacggtg tggaatattt tttcacagat
     6781 gagaccggtt ttcagaccct gagggaacag ggaaaggtca tagaggacag agcttataat
     6841 acggtctatg gcatctggcg gtattttaca gtggatgacg gacagataga tttgaccacg
     6901 aagaattatc tcatgatcgg aacactggaa gcctatgaga aaatgattgc ttatttcgga
     6961 acagaagcta ttttgccggt ctatattgag ctggatgacg gtgtacgcct gcagcgggca
     7021 ctggacaggg aactgcgaca ggagaagccg aagtacgaag agatgtgtcg cagatttctg
     7081 gcggacagcg cagatttctc cgaagaaaag ctggcggcag cagggatccg ggacagattc
     7141 gagaatacag atctgacaaa atgtctgaaa gatattacaa agtacttaaa agaagccgga
     7201 atatagccga aatactgata acagagaaga aaaatgccgg aatatagccg gtaaacagag
     7261 gaaaagaggt gggcgtatgg aaatgagagt aaatcagata cagcagccgg cttccgtgga
     7321 gcaggtacag aacacagcgc ccacggatgg cagcttcaag ttcatgctgg cgagccatgt
     7381 ggaagaagca gaactgcagg agagactgac gaacctgatg gcggagatca ccacagaggg
     7441 cgaaaagctg gccaagaaac gggatgtgaa ggatatgcgg cattatcgtg gactggtcaa
     7501 ggaatttctc aatgaagtgg tgaccagatc ccattctttt tccagagaga acttcctgga
     7561 tcggagagga cggcaccgtg tgtacggcat tatccggctg gtggatgaga atctggatca
     7621 gctggcacag gaactgatga aggatgaaaa agacaatctg gcgatcctga acaagattgg
     7681 ggagatccgg ggactgttat tggatatctt tacctgatga aaaatacagg aaacgcttgc
     7741 gtgattcgca agaaggcagt acgataaaag caggaccaaa aagacaataa gatacagaca
     7801 gggggaatac caatggcaac atttcatgac atcataggtc aggaacagat taaagagcat
     7861 ttacagaacg ccatttcggc gaagaagatt tcccatgctt atatcatcaa cggtgaaaaa
     7921 tcctccggca aagaatttat tgccagagta tttgccatga ccctgcagtg tgaaaagggc
     7981 ggaaccgagc cttgtcagga atgtcattcc tgtaagcagg cgttgtcgga caatcagccg
     8041 gatattatct atgtcagcca tgagaagccg aataccatca gcgtagatga tattcgtgcg
     8101 cagattaaca acgatattgc catcaagcct tacagcagcc cgtataagat ctatattatg
     8161 aacgaggcgg agaagatgac tccccaggcg cagaatgcaa tcctgaaaac gctggaggag
     8221 ccgcctgagt atgcagtgat catgctgttg acttctaacg tgaattctct gctgccgacg
     8281 atcctcagcc gttgcgtggt actgaacatg aagccggtag ccgatgaact ggtgcgtaat
     8341 tatctcatgc atcagctgca ggtgccggat tataaagcgg aggtctgtgt ggcatttgcc
     8401 agaggtaata tcggcaaggc gaaatccctg gcgtccagtg aggacttcga taatattaag
     8461 aatgaagcgt tgtcgctatt gaaatatatt caggatatgg atctgagtga gatcaccgca
     8521 gcgatcaaaa agattacgga atataagctg cagatcaatg attatctgga tctgatcgcc
     8581 atctggtacc gggatgtgct cctgttcaag gcaaccagtg atgtgaacca tctcgttttc
     8641 cgtgaagaga tcagtgccat ccgccgtgtg gcccagcgca gcagctatga aggaatcgag
     8701 gaagtcatcg aagccctgga taaggcaaag aggcgcttgg atgctaacgt aaattttgac
     8761 ctgactatgg aacttttgat gctggagatc aaggagaacg gttaacagaa agacaggaat
     8821 aagtagaaag tgaaatttac gcttcacttt ctattttttt gaaaaaaatg accggttgaa
     8881 atctcaggac actttcccga aaagctattt gctatctgtt gcgattcgga tctggaaaaa
     8941 acggaaccgg aaaataaacc gcagaaacag cattgcagcc ggaaaatacg aaatcgtcta
     9001 cggtgtctat ccgaactgga ataagtacgg agcggaggaa ctttggcaga tggcggcgga
     9061 tgccagtgcg gcacacctgg agagctaatt taccggaatt aaattgcggt gtttgcttct
     9121 cagcatgtgc cgtcggtcaa tgcggcatcg gggaaaaatc ataaattccc tttatttaac
     9181 gaacgaatat tttctgcctt cataaaaact tcagcatatt cacaattcat aagaatgcca
     9241 ttggttcttg ctttaagtcg ataatattct acaattggca taaaaggaca attttgtaca
     9301 ctttgctcct gctcaagtgc cttttgccat agagaaaaac cttcgcttat gtcaaaaaag
     9361 gtttgcggcc ggaaggaact acaatcttcc cagttttctg tgtaataaaa tgctggtacg
     9421 ttgtaagctg gatactcaca tttgatggct ggtaaggatg cataaaaaac agcattatta
     9481 acaatagtat ggcaagccat atgatctttg tgtgtgcttt gataatgggt tattaaaatc
     9541 tgcggtttca gcttgcggat caaggcacaa aggtcaaggc ataaggaatc tgttggaaca
     9601 aggaaaccat cgggaatatc atatattatg ctttgaccat gtaattcagt aacaaaagaa
     9661 gtagcctcac gccttttcat ttctataaat ttgtctaaat catctgtggc actggcactt
     9721 ttttcacttg tagttaagga aataataacc ggagtccaat tttgcattgc acaggtagat
     9781 aaaagtcccc cggcagttaa ttccatatct ccaacatgtg ctccaatagc taaaataaca
     9841 ggggaagtag gtttattcat gatgaacctc cttgctgatt attttttcaa agaaatctgt
     9901 gggaattggt aattccatag ctattagaca attaacaata tcacgcagtg actgtacacc
     9961 aataaccggc acagaaggat atggtaattg ataaatgtat gccagaacaa tttgggtaag
    10021 atcccagccg gtttctttgg caagcttttt aatagcggta agtcgttccc ttgacttatt
    10081 gttatcaaat aattcccgac aggatcgata ctgcggcgcg gtttgaaagt ctgtccggtc
    10141 tgcaagtgaa aaataacctc tggctgctgc tgtataagca aataacggga attgattatt
    10201 tgtgtgtaat tgttccagtt cagaagaaat atatgtcatt ccatcatata aaacagcggg
    10261 catgttggga tacgcaacat tcttcatagt ttgatgtgct gtaaagccgg tgattttttt
    10321 atcatcagcg taacgcatag cttcatgaat gcgctctgca ctccagttag agcatccaaa
    10381 atagcgaatt ttcccctctt ttttgaattg attcataatt tcaagtattt cacccgcagg
    10441 aacagcagga tcatcaacat gaacccagta atagtccaga tagtccgtat gaagattgga
    10501 taggctgttt tccaaatcat ggcgcagaca ctcggaatta attcggggga tggatttatc
    10561 ccaaggattt aatgcgccac ctttactatc cagaataaag tggtgccggt tgtgcctttc
    10621 ggaaaaatat cttcccaacc acaattcatt taccggaagt ctttgctcaa accatcgacc
    10681 gtatacattt gcagtatcta aaaatgtgcc accaagttcc agatatgcat ctaccagata
    10741 ataattgtgt gtgtcggtgt aatcactgcc ggctggagca aaacttgcag ctattcttgg
    10801 tatagtgata tttgtttcag gaatgtgtat atgtttcatt gttgtacctc actttcatat
    10861 tcagggtatg aatagaggaa attaccgatg aagttaatat tttttacgat agttaacagg
    10921 tgtcatattt gtaatttgtt taaatgaagc tgtaaaatga gatagattaa tataacctaa
    10981 acgttgtgca atttcactta cactcaaatt tgtggtgcgt aatagcattt tggcgtaatc
    11041 aatcttcttt tccttaatat attgagagac agataatcca aattcttttt tgaaaagacg
    11101 atcaagataa tctgggttca tatgtatata gtccgcaacg gtatttcggc agatttcctg
    11161 ttccaaatgc tctttaatgt aattttgcac tacataaaca agcgaactgt ttttgggtaa
    11221 taagctctca ttaacttcag caaattcaaa aatattttgt ggaccatgta gatctaattg
    11281 gagcatatct ttcatctcac ccagcaacac ggggattttg gcattatatc cggtgacaca
    11341 tagcagtgga aggctatttt ttacgagggc tgtatacata tttttaatct gttgttttac
    11401 ttcctcaata tattgtacct tgcaaggaaa caggataaaa atgttttgga gctggtagct
    11461 aaagacaaca gcatgtggac taaatgtttg tacttctgtg taaatttgtt tttgaacgtt
    11521 ataacgtaaa tcggcaactt gttgaatatt attacattgc tggcggtcaa taataaaaag
    11581 taccggataa tagagtatat ccaacatggt ctgagacggt aagatatact catgaccgga
    11641 aatataggag aaaagcatat cgtgccataa actttctttc tgcaaatatt ggtttaaata
    11701 aagatcctta tctggatgat ggagtttgtc aatacatttt tcaatagatg cagttatttg
    11761 ttgtggtgtt atgggtttta gaatataatc caggcagcca agccgtattg cttcctgggc
    11821 atattgaaat acactgtaat tagtcacaaa gacacaacct gtttgcggat aattagcccg
    11881 aatccaggaa agcaaggcta atccatttgt ttggggcatt tcgatatcac acataactaa
    11941 gtcaatcatg ggctcattta aaaaatatct ttttgcatct tctgcgctat aagcaaggag
    12001 tattttttca atatttaatg attctatgga aatagaacga gctaatgttt gaatgattat
    12061 tggttcatcg tcgaccataa gcaatatcat aaacaaaatc ctttctaaag ttcaattgga
    12121 atctttactt ctgcacaggc tccgccggtc ttcggattac acaggtgaat aaatgcatta
    12181 ccgtcataaa aatagtagaa cctgcttaaa agatttttca agccaatatg ttcgtttcca
    12241 atggaaaaag gcggtgtata attttcaata tcttgcaaaa gtgaatcggg gaagccaacc
    12301 ccattgtccc ttatcgtaag tataaggtaa taagaagcat cctcttgctg aagtatggca
    12361 gatacattta gttttaggag cttgagctgt tcatgtgcat attttattga attttccaaa
    12421 aaagtcaata tggtaagcgg aggaatcata cagccagtca atgaatcggg aatattgaaa
    12481 tgtacctgtg gcttttgcgc tttttgaacc tgctgtatgg aaatataatg cgagcattgt
    12541 tccagttctt ttcttaaagg aatgagggat gaactgcttt taaatacagc ccggaaatat
    12601 tccataaatt ctaaggtaag agactgtaac tgcaagtttt cttcatttat ggagagaaaa
    12661 taaatggtat ttaatgtatt tataaaaaaa tgagggctta actgaagctg cagacattgt
    12721 aaatccagtt gagtgcgctg cagttctttt tcataaagct caattttcat tgtctgtatt
    12781 ctatgaatca tggtgttaaa agaagctgcc atatgatcca tctccatgct tcccatattt
    12841 tcagtcagtt cggaagaaaa gtcattatcc cgtatactat ccatatgttc gataaatata
    12901 aggattgggt ttaatatatt tctttgtaaa tatgtatttg ctattggaat taaagatagc
    12961 aaacatagtg agaaaaaggt cgtaatccaa cataagacaa tagtaatata attctgtaat
    13021 ggaatgtcag cagataaaaa tactgaaaaa tctccggtgg atgaatcagc tgtaatttga
    13081 aaatgccgta agagaaaaaa atcaagcata ctatttttgt ctgtaaaagt accggttggt
    13141 gcaattgtta cagctgtgtt ggaaaaactg taatttttta acttttgaat catggaatct
    13201 gtcggtacac agaccccaag ataagcgtta ttacatttaa ataagcgcaa caaaaactgt
    13261 tgatcctgca gtgtaattaa tgtccaggaa gcattcagaa actgttcagg atttttcaac
    13321 atttcgcaaa gagtctcttt tgcctgataa ctgccatgcg acacatattc tgaagaaggg
    13381 acgatgacca tttcgttgtt ttcagaatga aacataaata aataggcaat ttccgggcaa
    13441 agatcaattt ttgaattaag attttggtat aagtgaattt tttccaatgc ataatctctg
    13501 ccgacataat cataagcaat gctttgaaaa gctagatcct ctgttccgtt ttttatgaga
    13561 aagaacgaaa tcgtactcaa acgagaatca atatcgtcta aaaaataatt aaccatattt
    13621 tcagtagata tttttgtttg ccgataggtg gaaaacataa agaaagaatt ggttatgatt
    13681 aaaagaatat ttagtggaac tacaaaaaga aaaataaatt tgtttacagt ctgctttaag
    13741 gttcctttca taatcgtttc ttcctcattt ctaaagcttg gtatttttga catgtagcac
    13801 tatatattat atagcatatt tagaatatta tttgtacata atgtcaaaaa ttggataaaa
    13861 aaagtcgaaa atagggcaga tatgacgaaa gaaagtatga tagattatgt gcacagggta
    13921 tgaaagaggt ttaaaaatct attataaaga aaaggagaag attaatgaaa aaaagaaaaa
    13981 aatatgtagc tattatgtgt gctgccttgt tggcgttttc gtcgttgctg caaggctgtg
    14041 gaaaaacaga aacggcatct aaaaattcaa ccaaagaagt gttgtcagaa tcatcggagc
    14101 aacaaagcac agaaccggac aagataacat ttgcattttt ctgcaattta ttcattccgg
    14161 aagatatgga catgattgaa gaggccttaa atgaacagac tcgtaaaaag attaatgtag
    14221 aggtagaact cgtgccatta agccttggta cttataacca gcaaataaac ctaatggttt
    14281 ctggtaatga aaagttagat ttatatatta tgtttggtgg tgatttcact tctgcgatta
    14341 atcagaataa gcttctgccg attgcacagg atacactgca ggaatatgca cctggggtag
    14401 tcaatgcttt aggggattat ctgaaagcat caacagttgg ggacaaaata tacggtctgc
    14461 cggtaaataa agatatggca caggcaagag gaatttcttt gaacaaagag attctggaga
    14521 aatatgacct ggtagaaatg ggagaaaata tttccaatac agatgatctg gcggaattat
    14581 ttgcaattgt ttctgagaag gaaccgggta tggttatgac gtcttctcag gataatggaa
    14641 aaagtatact ggaatccgga tttgcttcat ttgataagat gggcgattac tatggggttc
    14701 tgatgaattg cggcgaaaat gactatcagt tggtaaattt gtatgaaacc gaatggtata
    14761 aagatacgct gaattatatt cgtgattggt acaataaggg ctggatttta agtgatggtt
    14821 ctatcaaccc ggatacgggt atgggcttat ataagtcagg aagactattc tcctacatgg
    14881 gagataaaaa gccgggcggc tatgtagatg tacagaattc aacagggatt cctaccctgg
    14941 atgcgcgtat gtcacaggcc gttgctacaa cggattccat taatggagtt gttatggcta
    15001 ttcctattac atgtgaagat gagatccctg tattaaagtt tctgaatttg ctgtattcag
    15061 atccggatgt gcttaatatt attgattggg gtattgaggg tgtacattat gaacgtgtga
    15121 aaggtaccaa cactttaatt acttacccgg aaggtgttac ctctgaaaat gatggatatg
    15181 ggcttaacca gggatggtcg tttggtaatc agcttatttc ttatgcatgg gaaacagtag
    15241 aaaatgataa ttattatgat gagatgaagg ctttcaatga aaatgcaatt cagtcgcagg
    15301 caattggttt tatatttgat tcctcttcag ttaagtcaga aattgctgct ttggatagtg
    15361 tattagcaga atatcgactt ggtttggaaa atggtgaatt agatccggaa gaatatttgc
    15421 ctaaatttat acaggctctg cgtgaggctg gtattgaaaa ggtgattgca gagaaacaga
    15481 gacagttgga cagttgggtg gctgagaagt aagcagtatg gaagcaagac acggtttatt
    15541 gtgatttcgt cagtatcgac atctgttaat aaaataaacc gtgtcttatt gtctctaaat
    15601 taggaaggtg atttattgat gaaaaagcaa aaactgaaaa aaatgaaacg gcagctgcca
    15661 ttatatttaa tggccttacc gggaatttta tatttattga taaataacta tttaccaatg
    15721 actgggcttg tggttgcatt taaagaattt aattatagag atggtatttg gggaagtccg
    15781 tggaatggtg ttaacaattt taaatattta tttagcactt caacttcatt gattattacc
    15841 agaaatacac ttctttataa tttagcattt atagttttga atacactggt tgcacttaca
    15901 atagctatac tgttgaatga aattcgtaag ggcatacaca ttaagttttt ccagacagtc
    15961 atattacttc catatctttt atcaattatt attgtgggat atctggttta tgcttttctg
    16021 ggaaatgatc ttggatttgt caatacacaa attcttacca aactgggaat gaataccatt
    16081 gagtggtata gtcaaccgaa gtattggcct tttattcttt tatttgtaaa tacattaaag
    16141 aattttggat ttcttgcaat tatttattat gcaagtgtca tttcaatcgg caaggattat
    16201 tatgaggcag cagaattaga tggtgcggga aagatgaagc aaatctggta tattaccctt
    16261 ccatttttaa aacctacaat tataatgatg gttttgcttg ctgttggtaa agtgttctat
    16321 tcagatttcg gattgtttta tcaggttccg cagaactccg gagcactgtt tagcgtgaca
    16381 aacacaattg atacctatgt gtatcgatct ctcattcagc tgggtgatgt gggaatggca
    16441 tcagcagcag gagtatatca atcaattgtt ggatttattc tggtgttttt aactaacctt
    16501 ctggttcgga aaattagtcc ttcaaatgct ttgttctaaa ggag
//
LOCUS       DAWEJW010000063        16194 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1332_length_16194_cov_63.290600, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000063 DAWEJW010000000
VERSION     DAWEJW010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16194)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16194)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16194
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1332_length_16194_cov_63.290600"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2478..2577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3477 a         4561 c         3206 g         4850 t
ORIGIN      
        1 gtcatgacac aagaaaagct ccctcacagg aaggagcttt gtctacagtc tgaaaggcat
       61 actcgcgtat gcctttttct accgtatcat cctataatca acctttcatc tgagcggcaa
      121 cttctgctgc gaagtcttcg ttcttctttt ccatgccttc gccaacttcg aaacgaacaa
      181 ccttagcgat ggtcagagcc ttgttaacgc tctccagata cttagctacg gtctgcttgc
      241 catcctcagc cttaacgtag gtctgatcta acaggcagat ctccttcagc tgcttggata
      301 cacgaccgtc tgccagaccc ttgaagatct tgtcagatac gatagcgtcc ttgtcagcca
      361 tagccagctc agccagagca gctgcagctt ctttgggcag aaagttgaac aggtagttca
      421 tgttgctctt cttctcttcg tagatagcct tgtcctcatc gctccatgca gaactgttca
      481 tagccttgtc gatcagcttg ttcagaatgg gcttaggcag ggatgcagga tcgttcagag
      541 ctgcgtcaac agtgatctcc tgtaacttag ccagctcttc tgcggaaata tcatttctgc
      601 tgatgtaggt aggattcata gctgctacct gcatagcgat gttggtcagt gcttctcttg
      661 ctgcgtcatc agatgcgccg ttagctgcaa cgataacacc gatacggccg ccaccatgga
      721 tgtaggatac tgctacatct ccggctacct tctcgaaacg gcgaatggac agcttctcac
      781 cgatggtagc agtcttctct accagagcat ccttaacggt tgcgccttcg aaaggaactg
      841 ctaacagtgc ttcgatatct tcgccatcct tgccaccctg taaatcggaa gcaagtgctg
      901 ctgcattaac agcctctacg aaagaagtaa aggtctcgtt ctttgcaacg aagtcggtct
      961 cggagtttac ttcgattacg caagcggtct tgccgtctgc ggagatagct gcacgagcaa
     1021 caccttctgc tgcgatacgg cttgctttct tctcagcctt agcctgtccg ttctttctca
     1081 ggaactcaac agctgcgtcc atatctccat tggtctcgtt cagagccttc ttacaatcca
     1141 tcataccagc gccggtcatt tcacggagct cttttaccat agatgcggta attgccattt
     1201 ttttatcctc tcttcttaat taatatatta tcctcaagaa cccagaccgt aatgacaggc
     1261 atcgcaatct gggttcctat gcttttctta tggaatcgga ttattcagcg tcagctgcaa
     1321 cctcttcgat atcctcagcc tcggtagcaa cgtcagcgtc actgtataca gcttcgccct
     1381 gctttgcttc gatcacagca tcagccatct tggaaacgat cagcttaacg gctctgatag
     1441 cgtcatcatt accggggatg atgtaatcca gctcttcagg atcacagttg gtatcaccga
     1501 taccaatcag agtgatgccc agtgcatgag cttcctgtac gcagattctc tccttcttgg
     1561 gatctacaac gaagatagcg tcgggaactc tggtcatgtt cttgataccg cccaggttct
     1621 tctccagctt ctcccattct ttctttaact gaataacttc cttcttaggc agtacatcga
     1681 aagtaccgtc ctgagacatg gtctcgattg catgcagtct tgcgatacgg gactggatgg
     1741 ttctgaagtt ggtcagcata ccacccaacc atctctcgtt tacatagtac ataccacaac
     1801 gctcagcctc aacctttaca gcgtcctgtg cctgcttctt ggtaccaacg aacagaatgg
     1861 tgccaccctg tgcggcgatc tcggatactg ccttgtaagc ctcgtcaacc tttacaacgg
     1921 acttctgcag gtcgatgatg tggataccgt ttctctcggt gaagatgtaa ggagccatct
     1981 tagggttcca tcttctggtc tgatgtccaa aatgaacacc tgcttcaagt aactgcttca
     2041 tagaaataac gctcatgtct ttacctccat ttggttaaaa tcttccgcat ttcttttctg
     2101 ccgggacgcg ttaagaaccc ggttagctct ggcgaagacc tctgcatttt ggcaggagca
     2161 ccatcgtcaa atcgaaaggc gtgttttttg tcacaacagt gcgattataa cataggaaaa
     2221 cccctatgac aaggggtttt taatgttttc ctgtgattat tttagcaatt tcctcttcgg
     2281 aagtcccggg agcgatctca tatgtgccgg gattgatact gcggctgtag ccgttgttgc
     2341 ataaataatt gtcaaatgcc tttgcattct ccaccagtcc ggcctcatag agccgtctgc
     2401 tggcactgcc ggaatcatcg cctctctgga ttgttatgct ggcggtctgt gtccgctgtg
     2461 gtgcttcggt tacttccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt
     2581 gtctccggtt ccgccgtagc ctgtgtctct gcgggaaccg tggtctccgg ttccgcttct
     2641 atattgttct gttcctgtgt ctgtgtggtt tcctccggtg tgccttccgt cggctgtttt
     2701 gcactgtttt gcagtgccgc cagcgtcaat tcactgctgt ccaccattcc aagttctgcc
     2761 gcacgctctc tgatctggtc atccgtcatg atctgggatg tctgatcctg ccggttggaa
     2821 aaactgattc ccaggatcaa tgccgtcacg atcataccga ttccgagacc tcgcagataa
     2881 tattttaatt tcatcgatat ctccgtcctt aattcacacg attactgtca ggccttatac
     2941 aggtcgatca caagctttac ttcaccgaca cccagcccca gttccttggc aatcgccact
     3001 gtggatttgc cctgtctgtg catttccaga atcttctcgt tattgttctg accttcttct
     3061 gcagtctccc ccatgaatcc gatctccggg gagccggacg gctctatcgt ctctgccggt
     3121 tcctgtactt ctatcagctg ttcttccgtc acattctcct tctgtgtctt cacgttctct
     3181 tccgctttga ccttagcctt gtcttctgcc gccttcactg ccttatccat tttggatacg
     3241 gtatctttga tattggcatg cttattgttc agcatatcgt ataaaaacat ggcttcctca
     3301 tggttcttgt gaatctcctg caataccgta tcggaatatt cactcactgc catgatcttt
     3361 tcattggtca gacgttccag ggaacgttcc gccttctcca gcgcatcttc cgaggtctcg
     3421 tccattttat cctgcatctg actgcgaact gtctgcattt cttcttccat aagacctttg
     3481 atctcttctt ttgctgcttc tttattgatt ccgctttctt ttccggaagg caataaaaaa
     3541 cttcctataa atattacgat tcctgcgatc agcagaacca cttctaatat acccatactg
     3601 cttttccttt gtattctcag atcttaatgt caaatccctg atgctgtttc gtcaccacca
     3661 caccatcttt ttcggaattt tcttttttcc ggtgtttacc accgttaccg gcatactcat
     3721 tctttccttt ttccttggca tcatactgcc ccgtatacca gtctgcatta tcactgctgt
     3781 gcacttctct ggtccgctgt tcactctgtt tctgtgcatg ctctcccagc gtcatctgtt
     3841 gaaatgcacc cttattgtct tcattctgct tcatcgtcgt gtaatcctgc gttcgcggga
     3901 tcgacgcaac ctccaaagat ccaaacgaca taagacattc ctcttttctg ccattttctg
     3961 ttcctgcaac aatctgctgc cggttacagt ggtgtggact tcacatctcc gtcaatgcgc
     4021 tcgaaccggc aataatgata ggtactcttc acctgcatgg aagaatctcc gatgatgatc
     4081 gtagtacccg ggtacacttc tccccgtacc tgcaccatgg cccgctcctg cggattgaac
     4141 tccagctgca gttcctgcat acgccggttc ttttcctcca gactcttatt gagtgtctcc
     4201 atggaagctg cagtgtttct gagatagttg atctgatccg gtgtaaaacg gactccctta
     4261 ctcttttttt ctgcaaaatt tgcaaggatc ggctgcagac tgcggatatt cttcacaatc
     4321 tcggagactt ccttctgcat cttcatatat tctgcctttt tctccggatc cacaccaact
     4381 tccactaccg tcggcgctcc cagcattgcc cccagcgtct taacctcaat cttctgtccg
     4441 gcacgtacat gaccgccggt gataaatcct cgcttgccgg ttacctggat ctctgttgcc
     4501 gctatcacat tgctgtgcag gatcgactcc gtgctgacat agcctccggc actggctgta
     4561 gcattctcca ggaattttgc cacaatattg cccccagctt tcaacgtacc ctttgccata
     4621 ccattcattc cgcgggcaat gatgatattg cccccagcct caatgtaggc accttccacg
     4681 acaccgctta tgatcacatt gctgcctgcc cggatgacga aattggagga aacattgcca
     4741 tttacctgca cgctgccctc aaaatcaata tttccggtag acagatccac attttccact
     4801 tcgtagacat ccgatacaaa cacctttccc tctaccaggg aaacatgtcc atccaccaga
     4861 gaagtgagtc ttctccggtc ttcggaaatc tctatattat gaccgaactc cagatgggct
     4921 tttttcacct gtctgggccg gatcttggca ccctgtacgg tagttccgta ctctccctcg
     4981 tcctccggaa tgacctctgc cagcagctgt ccctttttac aggggttgat catgttcaga
     5041 tggaaataat ccaccgttcc gtcatctttt aacgtcggct gtgcatggac atccgtatta
     5101 aaataatatt tgatataggc gtctgtgcca tgtctgggtt cttttccccg ggccactacc
     5161 agatcggtgc aataaattcc gtcagacata aaatgatcct gcagaacact catctggatt
     5221 ccatagacaa tattccggaa ccggagatct ttgatcacac tgtccattgt tgcccgtttc
     5281 ccggtctgtg aagccggata gaaccgtacg attgcctgca aattgtcctc actgacttct
     5341 atacgacagg attcctcgat ttccggacaa tcctttcgtt ccagaaagca gaccgcgtct
     5401 tcttctgcga gtatcgcctt tttaatggac atggaatcat aaggtatgga ttgtctgtcc
     5461 agataatcca ttacctcctg cagtcttacg tcctctcctc catccttggg ttggaagatc
     5521 tgtaatccat aacctcccgg tgttctcacc acttgaaaat agccattctg catacgcctt
     5581 ttcctctgcc ttcccatccc gcattccatt acgggtcaac gaggttctac aaaagtccca
     5641 tgtagtctcc catttttgtc ttcattttct gtaaagcacg ggtatgtagc tgggataccc
     5701 tggactccga cacctccaga atgttactga tttccttcaa tgtcagatcc tcataataat
     5761 atagcgtaac tactttcttt tccttttccg tcagaagttc cagtgcttct cccagaacct
     5821 tggtcagttc ttctttttcc acactttctt ccggaccatc aaaacgtgct gtcatatgtc
     5881 tgctataatc ctgtgagaca tcacttccct gatccatata ctcgttcagg gatatcaggc
     5941 cggtgatctt catctgggac tgccagtcca gatattcctc tgaagaaatt cccagtcccg
     6001 ctgcaatgtc ttcatccgtt gcactgcgtc ccgtggtctg ttccacttcc ttgatgaccg
     6061 cgtcgatctt cttctgccgt tgtcttatcg ttcggggaat ccagtccatt ttacggatct
     6121 ggtccagaat ggctccccgg atcctcagac tggcataagt ctcgaacttt acctctttga
     6181 ggcagtcgaa cttgtcaatg gcatcaatca gtccaaaaat cccgtaactg accaggtctt
     6241 cgtaatccac attatagccc agatacatac tcagccgtcc cgctactgcc ttgactagcg
     6301 gagcatactc cagtatcaac tgttctctgg tctccggtcg tttattagca gcatattctt
     6361 ctaataacct tttcttaccg gcttcatcca ttcgaatcgc ttacaccctt ctgcgctgtg
     6421 tcctcctgtg acccagcagc ttcttcctct tgtaaagcct tctctttttc ttcctgctcc
     6481 tgttttgcct tctcttcatt ctgtctgtcg aaccggtcca gcaacagccg gatcacactt
     6541 cccagacaat aaaacagcag cataacagta aaaagtgcca caagacttgc cagtaacgaa
     6601 taacttctca ccagcgttac gatccaggta cacgcacctg cgatcagcat caaaagcaat
     6661 ggcatattct ttctgttcat aatttaccca tccggtcatc catccttaaa tgatggactc
     6721 actctttcct accgcacgaa tatggaactc tccggtctcc ggataaaaag ttacggttct
     6781 tccgtaattc ttacctgtat cctgtgccaa aataggaata ctgatctctt tcagtttctt
     6841 cttcacagcc tccacgttac ggtcccccac ctgcatcatg tcgtttttcc cctgaaacgt
     6901 gaacatagta gctccgccag caatcttggc aaccattctc gcttttttgg ctcccagctt
     6961 ctccatctgt cgtaccagtt catcgattcc ggtatctgca aattttgcaa tgttctgact
     7021 gctgttacgg atcgtagtgc tgtccggcag cataatatgt gccagaccgc ctatttttgt
     7081 caccgggtcg cggattgcga ttcccacgca ggagcccagt cccagagttg tcactccatc
     7141 tgggcttaca caggtcttca ggtccgccat ccctacttta ataatctcgc tcattctgct
     7201 ccccttcttc tgcgcttctg aatctatgct ataggtattc ccaaagatgt cagaatcttc
     7261 gggtaggatt caatatcggg aaccagaata aaatatccgt cgatctcaac ttcgtcataa
     7321 aactgagatt gtatcagcag aatcttgtct cccaatgttc cgaattcaat ggctggaaca
     7381 ctgagaatag ctcctgccat atccagtgtc agagacggtg gggtaggaat aatggtcaga
     7441 ttcgtcatca tggacaggga attcaggtaa gctcccgtaa taatgttgcc gatctcctgc
     7501 atggcagaaa attcgatctc ggagaaccct tcctgctgta ccatgtctcc catgatctta
     7561 tggagaagat gcttggcgct gccctgttct accagaaaca tcatgctgcc ggtgatatct
     7621 ccttccactc ccaggaagac accgaccatt acctgctctt ctcctcccat caatgctcct
     7681 acttcgttga aatccaaaag ctttacctgc ggtaccttca tatctacctt acagttcagc
     7741 atctgggaga gtgctgtcat agcgttgccc gctccaatgt tgccaatttc ttttaaaacg
     7801 tccagatatg tttccgaaac tttctctaaa ctcagatctc ccatggatca gtctccttta
     7861 tgcattttcc tccaatgtga tggaattcag atccaacaga gagatcagtt ccccattgtg
     7921 tttgcctaca cctgtaataa agctcttttt gctctcagcg gtattctgtg ataatttctc
     7981 aatctcactg cttcccagag tcactacttc ccttacttca tccacaatga ttcctacact
     8041 gccctgctgt tcctgcttca gaatgatgat tctggtagca cgggtcagtt cgtctgcctc
     8101 cagtcccatc ttcacgcgaa gactcatgac cggcaggacc tcaccacgca gattgatgac
     8161 accttttaaa tatgcgggta ccttgggcac tctggtgata tgctgcattc ttacaatatt
     8221 gtcaatgtat cggatatcga tgccgtactg ctcatctccg aggcggatca caatatactg
     8281 gaaaaaatcc tcctcaactt tacgctcgtc tccggccttt aaagtttctt tcatgctaag
     8341 ttcttccata actgtcatct ccctccttac agtaatgcgt tggcatccag gatcagtgct
     8401 acttcgccgt caccaaggat cgttgcacca ctgatgaatt tgcactgctt gatgtatttg
     8461 ccaagagact ttataacgat ctcctgctgt ccgatcagtt cgtcgataac cagacctgcc
     8521 atcttatcgc ccttcttgac aatgacaacg atcaggttgt cctcagggga tctggtactc
     8581 tctacatcca gtacctcggt cagacggatc agaggaatca cggttcctct caagttgatc
     8641 acttctttgt tctctaccag tttgatatcg ttgggagaga tatcttcgat cgtctggatg
     8701 gatcccagag agatcgcata tttttctcct cctacgacta ccatcagtgc ctggataatt
     8761 gcaagggtca gaggcagtcg aatggtccag gtacttccct ctccccactt ggatttcact
     8821 tccacttcac cgcttaagga ttcgatcttg gacttaacaa catccagacc tacacctctg
     8881 ccggaaacgt cggtcacctt ctcagaagta gagaatccag gctggaacag caggttgata
     8941 atatccttct cagacatatt agcagcctgc tccgtggtta ccagattacg ttcgattgct
     9001 ttattcttaa cagcttctac atcaataccg ttaccatcat cccttacttc gatgactacg
     9061 ttattaccat cctgataagc atccaggaag atagatccct gttccggctt gccacgctgt
     9121 gcacgtacct ctgcggattc cagaccatgg tctgcggaat tacgaagcag atgcatcaga
     9181 ggatctccga tctcgtctac tacggtacga tccagttcgg tctcttcacc actcatatac
     9241 aattccatct tcttgtccag cttcttggac agatcacgga tcattctggg gaacttcaca
     9301 acaacgctct caataggtac cattcgtacc ttcattacag attcatgaag gttcgtggtt
     9361 acgttttcca gatactcgat ctgctcattg aatgcggaat tattgccctg gtcattgctg
     9421 gacgcagcta ccagagagtt cttggcaatg atcagctcac ttaccagatt catcagggaa
     9481 tccagttttt caatatctac acgcacggta cggttgacga ccggcttatt ggcgggtttc
     9541 ttttccggtg ccttggctgc ggctacaggc ttcttttctt ccttcttggg ctgttctgca
     9601 gccttgacct ctgccactgc ttccttcttc tcttctgcgg gttcgattgc ttcctcttcc
     9661 gcatggtaat tcttggtctt ctccagttcc agtaccgcac cggttacctg tgcgatctca
     9721 gatacgctct ctgcggactt gataatatca tccagagagt aatcagacag aacgatcagt
     9781 gtgaagtcct tgtcgaactt ctcatcctct acatcctgtg cgctgggcat ggatacgatg
     9841 atctcacctt tttcctccac ggccttgaat accaggaatg ctcttgcggc cttcaggata
     9901 cagctttcct gtaccactac attgatgccg tatacatttt taccctgctt catagcctct
     9961 ttgatcacac ggatctggct gtcatcaaaa gcaatgtcat tccatttttc accaccgtct
    10021 gcgccggatt cagctgcagg tgctgtggca gtgggagcct cttccttggc agcgggagcc
    10081 tcctccgtct tgccatcgtt taaaatatca ttcaaggcct tgatcagatt ctcattgtcg
    10141 ttggttccct catcagaggt ctcctgaata ttgttcttgt actcttccag agcatccaga
    10201 cactggaaca atacatcgat catttccggt tttactttaa tattgccgtt acgtacttcc
    10261 gagaacacat tctccatgtc atgggtcagg ttctgcatac gcttgtagcc catggttcct
    10321 gccattcctt tcagggaatg cgcggcacgg aatatctcat tgatattgtc catgttttca
    10381 ggatctgact ccagttccag aatctgtgtg ttcaagttct gcagatgttc atttgtttca
    10441 tccagaaaaa tttcaagata ttggcttacg tccatctctt cctccattct gccggtcacg
    10501 gtattgccgg aaacggcata tattgcttgc tactttacta ctcctacatg cagtatgatc
    10561 tcctgggcga tctgatctaa cggtactacc tgatctgcca gcccggctgc gatgatactc
    10621 ttaggcattc caaatactac gctggtatcc tgatcctgtg caatcacata ggactttttg
    10681 gaagctttca gtttcccgat tccttccgtt ccgtccgctc ccattcccgt cataacggca
    10741 cagactaccg aatcaaagcg gctggtctgc agggactcat acatataatt ggcacagggt
    10801 ctgacacctt ccctgtaagg ttcctccgac agatgcagcg tatggcgtcc agccggggat
    10861 gtgacaacat taaggtgttt gccgcccatg gcgatgtaga cccatccctt tttcagttct
    10921 tctccctctg ctgcttcctt tacatgaagc tggctcaaat tatccagccg ttctgcaagg
    10981 gatgctgtga atcccacagg catatgctgt actaccagca ccggtgcatc cagattcgcc
    11041 ggcaataagg gaatcaccga ctggagagat ttcgggcctc ctgtggaact ggcaatggct
    11101 acgaccttgc tacccgacac gctgaccgga tgatgattga tgatcttcat catctctttt
    11161 accgtgcctt tttcctcggt aagcttcgct tcctcggcaa ataccggcag tttgctgttc
    11221 gctaccacat tcagtgtccg caggaattct ctgcggaaat tctcaccacg acagtctact
    11281 gcgctgtccg gcttatgtac aaaatccaac gctcccagtt ccagagcatc cagagtggtt
    11341 ctggcgcctt ccttcgtatc ggtgctggcc atcatcacac gggcgggaat cttgtattta
    11401 cgcagctcct gtaaaagctg caggccgttc atacgcggca tattcacatc caggacaact
    11461 gcgtcgtaac tgtttcggct caacagatcg aatgcttcca atccgtcttt tgcccggtca
    11521 gccacctgga accttatgtc agtatctatt atatcacaca gcactcttct catgagtgca
    11581 gaatcatcaa caaccagtat tttttttggc attagtccaa tctctccttt ttacgactct
    11641 tacgctcttc ttcaaaaata aagcggatga tttcttctct tgtatttacg tccaggttat
    11701 agtactgtac ccggtgttcg aacgtcccct tgcgtttctc cagttctctt accgccagta
    11761 ctttgcctac cacctcgtac tttttctgtt ctccgtcttt caccagatga taactgcaca
    11821 ggatcagact gccgggttca tatcgctggg tagacacaaa tcgcagtcct ccgccgctga
    11881 tgtccacgat cacgctgtgt ttcaaaggaa gtcccggctg cagttcataa ggagaatttt
    11941 tctccagtgc ctggatctct tcttcctcca gatttctggc acacatatcc agtgcgcagc
    12001 tgaaacgata atattctctt ctctgatatt tccgcagatt gctggtcagt tccatgacca
    12061 gcacatatac gttgttgctt ttgtaccggt ctatgatcct tgcaaagcac tggtacaggc
    12121 tgttctcctc gaaaaagatc atgtcgtatt ccgagtccac cggcaaaagt atcagtctgg
    12181 tctgctccat gggcatggag atctccaggg tatcctccga cagaatctgc agcacccggc
    12241 tctggtacac ctttgccgtg ctttctgctc ccttttcctc caacccccgc tgcaatgcgc
    12301 ggagctctat tttcgatcct tcttccacaa atctggataa cataagttca tacctctctt
    12361 atctggactg ttttgtagct ccatttctcg tacctataat gtgagaaaaa aatgctgcca
    12421 ttccgcgcct gggctgctgc tcctgcgctg tatcgtcttc tttttcgcac attctctctg
    12481 cgatcctctg ataagcctgg cttgcctttg ctcccggatt ctgtatggat acgggtacct
    12541 gttgcatcac cgcctgcatg acctgaggat cctgaggtac acatcccata taagtcatgg
    12601 gaattttcag gtatctggtc acgactgcat tcagcttgtc aaacagtacc tggccttcct
    12661 tgatattact tacccggttg gccaccatca tcaccttcgt gaaatcctcc tggaatctcg
    12721 gatgtctgta cagtgccttc agcagagaat aggaatccgt gatggaagta ggctccggtg
    12781 tggtcaccag cagcacttcc ccgctggcca ccagaaattc caacacactg tcagagattc
    12841 ccgctccggt atccacgata atgatatccg tcagggcatc cagctccgcc agattctgta
    12901 tgatacaggt cagggcatct tttcccagat tggacatccc ggcaattccc gaaccaccag
    12961 aaataaatcc cacttccata gggccccagg taatgatatc actgatgctc ttgccctcgt
    13021 aaaccagatc acacaggttg tgcttcggta ttgctccgaa catgatctcg atgttcgcca
    13081 gcccgaaatc agcatccaga atgatcaccc gtttgcccat tttgcggaat tgaattgcca
    13141 gattgatgga catattggat tttcccacgc cacccttgcc ggaagtaacg gtaatgactc
    13201 ttgcagtagg tcgtatttgg ttatttgctt taatgatcct tagctgttcc gcctgatcca
    13261 ttttcacgcg cctttctgtt aaagctttct tccttcctct ctattcctta cctccgagga
    13321 tctgtttcac cgtcttctga ggattgaaca tttccatgtc atccggaacg ttttgaccac
    13381 aggtcacata tgcgatcggt gcatccgtat aaagctttat gttcagcaga ttccccactg
    13441 cttctgtttc atccaacttt gtaaaaatca actcaaactt tgcgatcttt ccgtaacagt
    13501 ctgcgatctt ctggagatcc ctgtattttg tggtagcact taataccaga aatacctgat
    13561 agtcctccgg tctcttcagt ccacccacca tttccttcat tttctccaac tgttccgcat
    13621 tctggtgcga tctccccgct gtatcgataa aaatataatc acagtcccag taatcatcaa
    13681 tggcggtctg caactcctcc ggtgtataga tcacgcggaa aggtgtctcc agaatattgg
    13741 cataggtccg caactgctcc gctgccgcaa tacgataggt atctgccgta aggagagcca
    13801 cctttttctt gtcttccaca caatattttc ctgccagttt ggcgatggta gtcgtcttac
    13861 ccacaccggt aggtccgatg aaccatacga ttctcggtcc gtcctccgcc ggagtgattc
    13921 cttcggattt tccgaatttc aacaccatct tctgataaat attcgccagt agataatcaa
    13981 agggaagatc cgctttcttt gtcttggaag catcctccag aatactgttg acatattttt
    14041 catccatctc attatccagc attttgttat acagcagacg gatgaactta tcctgctccg
    14101 gattctgctc gtcctctttt tcttcctgct tttccactgc tgcttccgct tcactgtcca
    14161 aagcttcttt ctgtgcttcc gctgccttct ccgtatttgc atctaccggt ctgctgttta
    14221 actgactttc caaaagcgtc tgcagactat ccagcttttt ttctatattt tccgtgctgt
    14281 ttttcatcga tacagtcttc gccgtaccta cacctgcccc ggaagcttcc gtacgattca
    14341 gactgtttac cacagatctc tggggggact catcctccag cgcagcggtc acctcgatct
    14401 gcttcggtga cagaaaagag aacagccctt tctttttcac ttcacggaca ttcatgatca
    14461 ccagtccgtt tcccaattct tttttcgcag cctctgttgc ctctgcttct gttttcccgg
    14521 taaatttctt aattatcata ctgttcctcg ttcctgtgtc ggttcaatgc gcttaatatc
    14581 ttaagctgtg accataccca cagactgcaa ttccacattc gattctactt cgttgtaaga
    14641 cactacgacc agatccggat aataatcttc ggtcagccgc ttgaaataca ttcttacgat
    14701 cggtgatgtc atgataatgg gggtctttcc catgttttcc agctttttga tctcttctcc
    14761 caccgcattg agaattgctt tgcttctggc gggatccaga ttcaggaaag ctcctgtctc
    14821 tgtctgtttt acgcttccca tgacttcctg ctcgatcttt ggatccagcg tcagcacact
    14881 ggtagtctcg tgggaaggga aatacttggt ggagattgct ctctttaagc tctgtctcac
    14941 atactccgtc aggatatcgg tatcccttgt cgtcggtgca taatccgcca gagtctccag
    15001 aatcgtcagc agatcccgga tggagattcc ctcccgcaac agattctgca atactttctg
    15061 gatctcgccc agtcccaaca actttggtac cagctcgtcc accagagaag gattgttctc
    15121 ttttacattg ttgataaggt tctgtacatc ctgtctggtc agcagctccg caatgtgctg
    15181 gcggatgatc tccgtcagat gcgtagcgat gatggaaggg ggatctacta ccgtgtagcc
    15241 caggctctcc gcccgctctc tctgaccttc cgtgatccag atagccggca gatggaagga
    15301 aggctcgaac gtggggatac ccgtaatctc ctcttccaca taaccgggat tcattgccat
    15361 ataatggtca aacaggattt ctccctcact gacctgaata cctttgatct tgatgatata
    15421 ctggttcgga ttcaactgga tattatcacg cagacggata atcggcacta ccgtaccaag
    15481 ttccagcgcg atctgtcttc ggatcataac cacacgatcc aacaggtcac caccctggtt
    15541 cacatccgcc agcggaatga taccgtaacc gaattccagc tcgataggat ccacctgcag
    15601 gagactgttg acattttccg gctgccggat ctcctccgcc gcaacctctt cgctgtccac
    15661 ctcctgctcg atctgggctg cttctatcgt accctcgata tttcttgcta cgatgataaa
    15721 taccagtccc agtcccacga acaggatcgt attcagttcc gtcacaaatc ccagaattgc
    15781 cagcatggct cccaccaggt acagaccctt gggtacacca aacaactggc ttaccagtgt
    15841 cgtaccgaag tcagcatcct tgcttccctt ggtcaccaga ataccggtgg acagtgagat
    15901 caacagggag gggatctggc ttaccagacc gtcgccgata gtcaggatag tgtatttatt
    15961 caacgcactg gtgatatcca tgccctgcct cgtcattccc atgatgatac cgcccacaat
    16021 attgatggtg gtgatcagaa gacccgcaac cgcatctcct tttacgtatt ttacagcacc
    16081 atccatggaa ccgaaaaagc ttgcctcttc ctggatcttc tctcttctct tcttcgcttc
    16141 cgcatctgta atggctccgg tgttcagatc cgcatcaata gccatctgct tacc
//
LOCUS       DAWEJW010000064        15918 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1354_length_15918_cov_57.323646, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000064 DAWEJW010000000
VERSION     DAWEJW010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15918
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1354_length_15918_cov_57.323646"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9788..9887
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14323..14422
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3630 a         4226 c         3152 g         4710 t
ORIGIN      
        1 tcgccaatca tgcaagcatg ttggctctct tgcgctcatt atatcatatt caccgaattc
       61 cttataaacg gcttacactc cacggaatac accccgtttt tctgatgtat ctcctcgccc
      121 cgcagaagct ttaagatcac ctcgctggca cagtatccga tgtcatgtaa cggcagtccc
      181 atggtggtca gctccggctt ctcatagctt gccatttccc tgttatcaaa gcctgccacc
      241 gcaatgttct ttcccaccgt atatcccagt tcatccagtc ggtcataggc tccgcccgcc
      301 ataaaatcat tcatacagaa aatcgccgta gctccccgct tcaccaattc ttccgtatgc
      361 tcataaccgc tttcacgatc ccattctccg taaacgatca ttccgggatc gtacagcaga
      421 ttcgcttcga acaacgcact ctgatatcct tccagtcggg actgggtgtg gatactttcc
      481 tttttcccgg cgatgacacc gattcgtttg tgtccgcagg agatcagata ccgtaccagc
      541 tgctcggcac cgcccttatc ctttaccacc acagagggga tctccggctt gttggtaaat
      601 ccgtaagcca ccaccgtagg aatcggcaga tccgccggga tcacattgat cagtctctca
      661 tgagacgcca catagatgat cccctccacc tgcttcgaca gtagcttctg gatctccttc
      721 tgcacgatgt tccggtgaat attgtcctga taataggcat ctccgtattt tttatacaga
      781 cgtaaattca ccagcaggat ctgataatct tctttttcgc aatactccgt gatgccgtcg
      841 atgatatccg gcagggcaaa gactgtcata tcctccgcaa tgaccccgat actccgggta
      901 ttcttcgtct tcagattctg cgctacgatg ttcggcgtgt aattcatctc ctgaatcacc
      961 tgcagcactc tctttttggt ctcttcgccc acttttccct tgtcgttgat gatattggat
     1021 accgttgcca cggatactcc gcactctctg gcgatctctt ttatggtagg catagtgtcc
     1081 ctcccttttg taagcgactt gtctaccacc attctaacac aacttttccc tatatcacaa
     1141 caggctgtgt taattcttca taccggtcag aacgatacct tccacgaaac tcttctgtcc
     1201 gaagatatag atcacgatac caggcaggat tgtcatgaac gtcgctgcca gtgctaccgg
     1261 ccagttggtg ccgaaggatc cggtaaagtt cgtcagtgcc acggcaaaag tgaatttcga
     1321 catactgtta atgtagatca gctgctgcag catatcgttc cagatctgga taaacagcat
     1381 catggctact accacaactg ccggcttgat ggatggcacc aggattgtcc acaggatacg
     1441 cattctgccc gcaccatcaa tggatgctgc ctcgtccagc tccttcggga tcgtcatgag
     1501 gaactgacgg atcaggaaaa tattgaagaa accgccaccg gttaagtacg gcaggatcag
     1561 cggccagtag gtatccccca gccccagtcc cttggtccat accacgtaca gaggaagcag
     1621 ggtaaccatg ctgggcagaa ggatcgttcc gacgcagata ttaaagatca gcttcttgcc
     1681 acggaaacga agtcttgcaa aagcatagcc acagaaaatt gcggtcaccg taccaaaagt
     1741 aacggccgga atgatgatgg agaaagtatt ccggaaatat ctccagtatt cgaaggtctc
     1801 caaggctttt gaatagttcg cccataacca ctcatgagga atgaaggaag gcggatactc
     1861 gttcaatttt gccgtactca tcagggagga ccggaagatc caccagatcg gcagtaccac
     1921 taacagtgcc agcaaaagta cgatcagcgt tgccaccaga tccagaacca gttgtttccg
     1981 ttttttactc ggtgataaat ttgcttccat cttctattcc tcctctccaa ataattgttc
     2041 tttctgcgtt ttgaacagaa ctatggtaaa tatacccaca agaatgaaga agatcaccgc
     2101 atacgccgca gcgtagccca tcttgttctt ctggaaacca tacatataga tcacatagga
     2161 catgaacatg gatcgtttac cgggtccgcc gttggtcagc gccagtgccg ggttgatgac
     2221 ctgtaaatgt gtgatcaatg ctgtcaacac attgtagaag ataatgggag tgatactggg
     2281 gatcgtaata ttccaaaatc tctgccatgc attggcccca tcgatcttcg ctgcttctat
     2341 gtatacgccg gggacattct gtaatcccgc caggaagata acgatcaggt tgccactggt
     2401 ccatatggcg ataagcacca gacagggaat aacctgattc gggtcatcca ggaacctctg
     2461 cttcggaagt cccatcatcc ggagcacgaa gttgaacaga ccgaagttac cttcatataa
     2521 ccattcccat cctttaaata cgccgatagc cggcagcaga tacgggatga agaacaggga
     2581 gcggaacaat gtccggcctt ttatattcat gtttaagagg atcgctatga tcagtgagta
     2641 taccacggca ccgatcaccg ccagaaaagc ataccataca gttgctttga tagaatccca
     2701 gaaatagata tctttcgtaa aaatacgctg gaaattggca agccctataa attccggctt
     2761 cgtaatgccg ttccagtcaa acagacttaa tacaaatact gcaagtgtgg gaagatagct
     2821 gatcaaggtc actcccagga atgccggtat cagacagaga tatgcggtca gttcttcctt
     2881 tcttcctgct cttgttgtag cgttattttt ctgtttccct tttttcattg taaccctcat
     2941 ttcttcacac atttttgcaa agagtaactt attttcgtga ataattccaa atcttctgat
     3001 aacaacaggt ggccacatca ctgtaagcca cctgttatgt tgtcccaata cattgttgcg
     3061 gtaatagtat tactggttgt tagcctcgta gattccctgt aactcttcca gattctcatc
     3121 aattgcttcc tgcatggtct gctgtccggc ccatacattg gagaatacag tatccaaagt
     3181 agcgttgaat tcatcggtgc cgttcacata gtaccaggat gtggatacca tgttgtcctt
     3241 tgcatagtca acaactgcgc tcttatacat atccttatcg gggaaattag gattatcgat
     3301 ccacttatct gtcttctcag catcagtata ccaggaatcc agaataggca tccaggtacc
     3361 tgcttcgatc agagaccagg aattctcttc ctgataatac cactttaacc actccattgc
     3421 ttcctcagga tgcttggtag ttgcaaatac tacgttaggg ccaccggtac agatggtaac
     3481 cttttccttc atataaggaa gaacacctac gccatattcg aagtcagcgc tcggcccaag
     3541 gaaagtgccg acattccatg caccatcagt tgccataacg accttcttgc ttcccaaaga
     3601 agactccaga gcgtttgctg cactggttac gggaggtgcg cagtggtata cttcagacag
     3661 atctgcaatc ttctgtaatg cgtctacggt tgcgggatca ttgatggtgc actcagaacc
     3721 atccttgctg tagtaaccgc cgccgttgga cagtgcccat acttccaact gccagggcag
     3781 ggtattgatg gtacatccgt aaatatcaat attgttggga tcgaagtcag gatccagagc
     3841 atttttacca ttgatatcga tggtaagctt ctgtgctgtc tctacaaaag tatcccagtc
     3901 ccaggcatct gctgcacttg cgggaggagt catttccttg acatccagat tctgtttctc
     3961 acatacttcg tttaatagat caatgttgta ccacagattc agtacctcgt ttgctgcgga
     4021 ataagcaaca ggcgttccct gatatttgaa ggtcagtgca tccagtggct ttgcatcgcc
     4081 ttcgccgtac atggagctga tgtcagccaa cagtccgttc tctgcgaact tcagtacacc
     4141 ggcctcactc ataataccgg tatcgggaag ctctcctgca gttgccatgg cattcagctt
     4201 ggttacataa gtatcatggt cgatcttaac cacttctact tcgattcgat actgctcact
     4261 gttgaacttg tcagccacct tctgagttgc tgcagtctcc gtctcgttac cccactgtgc
     4321 ataggtgata tgtactctct caccactgcc gctgtcagaa ctcgtctgtg tctgtgccgg
     4381 ctggcttcct gcatctgcgg atgctgccgg tgcctgattg ctgttgccac accctgtaag
     4441 taccattgct gttgccattg cggttgcaag gactgtactt acgattttct tgtttttcat
     4501 acctttctct cctttacctt tctttattat aacgacaata ggttgtcgtt gtaaacacca
     4561 gattgtaaac gcttacacgt tcaccgttct acacctttac cttgacccac accaacattt
     4621 cgcccggtgt acggttacac cagagcccat agggaactgc tgtaaggtgc atctgttctt
     4681 tctccagttt cagctctccg tacaggtttc cctgttcggt ggaccggtta tggatccttg
     4741 tagcttcata ctgtaatgtc ggaatctctc ccgggagatc cgtctgcgga ttccccttcg
     4801 tgacctctgt gttctcttcc acatacaggt ctgccagata ttttccgttg tcggtctcct
     4861 ccaggcagta gaccacgggg ccgttcatca atgcacatct tccagcatcc gctctcactt
     4921 cgtcattggc ggcgatccat ctgggcttta caccgaaatc aatacagatt tcatgctctc
     4981 ccgccggagt atttatcagc agatagcctt tttccaaaac agcctttgct gtctggccat
     5041 ccactgtaat ctcctgtttc cccgcatatc cggggcggcg gatcttaaag gttacttcac
     5101 tgtcctgtct cgtctggatc ttcaccttac tatcctgcaa cagtgtggat tccatgccga
     5161 tctccacggt tccactttcc aggactgcct ttgcagtgga ggaaatgaac tggtggatac
     5221 ataccgcatt ttcttcctgt gcgtagatgt attgtccaag agacgctaac gtcctcgcca
     5281 cattcggcgg acaacatgcc acggagaacc atttctgtct gacagccttc acatgatcca
     5341 tgtaagtatg ctccgtgcag aactccggta cgacctccag aggattcaca taaaagtatc
     5401 tgtctcccgc tctgttgatg cccgccaaca ctgtattgta caatgccagt tccacggtat
     5461 cgtaatagcc cgcctctccg gtgactgcac tcatcctctg tccgaacatc atcagtccga
     5521 tggaagcaca ggtctccgaa tagttcgtgg tattcggcag atcgtaatcc gtggtaaacc
     5581 tctcccgaaa accactggat ccgatgccgc cggtcagata cattctgtga ttcaccgtgc
     5641 tgtcccacag ccgcttacag gcttccagca tctcggtgtc ctcgcattcc acagccaggt
     5701 ctgccatggc ggagcacata tacatggctc ttaccgcatg cccttccgct gtcttctgct
     5761 ttaccggttc tatatgtgat tgtgaatatt ccagatcgta atttttgaac tccgggaaaa
     5821 attcccagcc gcctctgtct tccatctcct gtaaaaaata gcttggcttc ttccctctct
     5881 catgaaggaa atacgatgcg gtgtccaaat actgcttttc gccggtcacc cggtaaagct
     5941 ttaccagcgc aagttcaatc tcctgatgtc cgggataacc ttttttctgt ccttcgccgg
     6001 taccgaatac tctgcagatc agatccgcat ttttcctggc gatgttaaga atcttcgtct
     6061 tcccggtggc ctgatagtaa gcaaccgctg cttctatcat gtgtcctgcc gtgtaaagct
     6121 cgtgtccttc caccaggttc gaccactttt tgtctggctc gttgactgtg tagtaggtgt
     6181 tcaggtatcc gtcggtctcc tgcgcccttc ctaccagttc gatcacttca tctgccagtg
     6241 cttcgtattc tctgccctgc cccccggcaa tacagaatgc tacggattcc agccatttgt
     6301 acaggtccgt gtcctggaaa actaccccct ttctctctcc cggcagctcc cccgctgcca
     6361 cgcggaaatt ctgaatacag taggtaggtt cggagtcctc cagctgatcg ttcaagatct
     6421 tccattgatg cggaatgatc ttctcactga ccttttgcac gtattcaccg aacagcttgt
     6481 ctgtgatcct gatatttccg tattccagct gcttcctgtg atgtcccata cctttctcct
     6541 atccttgttc gttccaaaag ttaatcgttt aatcttttgt gagaaaagaa acagccagat
     6601 ggctgttata ctgctgtatt cttcttttct gcaagttaat cgttttacct tttgatgacc
     6661 ttatcatagc accagatagg tgttttgtaa ataggtttta tgcaaatcat cataatatat
     6721 aagtttttcc actgtttttt gtgcattttg cacattttag tacaagctgt cagattctta
     6781 atcgttttac ttgcctaaaa ctcctaacca cttcagagcg ttccaaatgc cgtctttatc
     6841 tacatctgtg gtaatataat ctgccacttc caacacctgt ttactggaat tacccatagc
     6901 aatgctggtg tgggcatacc ttagcatcgg caggtcgtta ttgctgtctc cgatggccac
     6961 agtgtcttcc atggggatct tcaaatagtc cgtaatgtaa cggattgctg ttgccttgga
     7021 atatcctttg ggaacaatct cgtaaaaccc ttgttctctg tcgataaaat ccagtatttc
     7081 cgacaggtca tgcttcattc cgtccattcc cgccggagtc tctgcatagg catataattt
     7141 atcgtagttg cccggtgcat tggcatagcg atcgtatttt ctattctcaa tctcacgtgc
     7201 catatgacgg aaagtctcgg taaatacttc ttctcctctg ggagcatagt cttccctgct
     7261 gccttccaga accgcatcca catgatattt tttcatagct gccagaattg ccttagactc
     7321 ctccatggaa aaggtcttgt gcatcagccg ttttccatga tattcaatct ccgttccgca
     7381 gcctaataac aatccgtcga attccacctg tccggtaaga ttttccccta ccagccgttt
     7441 cgtacgcccg gtattgatca tgcagatatg accgttggct ctggcgatgc ggatggcttc
     7501 cttcgtgctc tctgacatca gttcacttcc tccgcatatt aaggttccgt caatatcaaa
     7561 aaatattatc ttcatctcag attcttaagc tcctctacca gatccagatc ggatttctgt
     7621 accttcagat tcgtggacac gggctcttca ccgggtcttg tgatcgtgat ctcgataatg
     7681 gttccttccg tgatcgctcc acccataaaa aatctgctta caaacgctgc aaatttggga
     7741 tgattactct cgaaggtatt cttcgcattc atcagtttca tcatcattcc gggatttatc
     7801 atagtagtct cctcttctaa tcagtaacta ttctgtcgcc ctataactat cacataatat
     7861 ctttacgaat gcaagcattc gatgaatatt tcgtggtagt tccatggttc tgaatagtta
     7921 caaaaaaaga aaggctgtcc gcaccatccg aaaacagtgt gtccagcctt aacagttgct
     7981 ttaattatgc aatcttggac ttagccagct ctgcaagggt cttgaaacct tctgcatcgt
     8041 taactgccag gtctgccagc atctttctgt tcatgtcaac gcctgcaagc ttcagaccat
     8101 acatgaattt gctgtaagat aaaccgttca ttctggctgc tgcattgata cgagcgatcc
     8161 acagctgtct gaactgacgc tttctctctt ttctacctgc ataagagctg gtcagagctc
     8221 tcataacaga ctgctttgct actctgtact gcttgcttct tgcgcctctg tagccctttg
     8281 caagctttaa tactctattg tgcttcttct tggcatttaa gccaccttta attcttgcca
     8341 tgtcttattt cctcctcgcc tgaattataa ataaggtaaa atcttcttca tattcttaac
     8401 attagttgca tccgtaattg caggctttct cagattacgt tttctcttgg tagacttctt
     8461 ggttaagata tggctcttgt aagctttaga tctctttaac ttaccggttc ctgttgcttt
     8521 aaagcgctta gctgctgctc tgcttgtctt cattttgggc ataacgagtt cctcctaaac
     8581 tttacttaat ctatcatgtc ggaatccgac actttttaac tgtacgaatt aacgcttctc
     8641 agttaaaaac atagtcatgc ttctgccttc taccttggga gctttttcca ctaccgcaat
     8701 atcggacagg ctctgtgcaa tatcatccaa gatatgcttg ctgttgttca tgtgtgccat
     8761 ctctctgccg cggaatctca gggtgatctt cactctgtct cccttggtca ggaactttct
     8821 ggctgcaccg atcttggtat tcagatcgtt ggtgtcgata ttgggggaaa gtctgatctc
     8881 cttgatatcg atgaccttct gcttcttctt tgcttccttc tcttttctca cctgctcgta
     8941 tttgtactta ccgtaatcca cgatcttaca aacaggaggc tttgccgtag gagcaatctt
     9001 caccaggtcg agtccggctt cctctgccat cttcatggca tctctggaag aaacgattcc
     9061 gagctgttct ccgtcttctc caatcagtcg tacttctttg tctctgatct gttcattaat
     9121 cattaaatcg ctaatggttg tgtacctcca taagaaaata tattaagcaa taaaaaagca
     9181 gatagcataa ctatccacct gttcccttca cggactgccc tcattcaaac aaattccaaa
     9241 cgagttctct gtctatgaaa aatctgaacg cttaccagcc tgcgccgtaa ggtgagagtg
     9301 gatactctgc ttgttgtgcc gtgttcttca cacgctcaaa tagaatacca cgaagttttc
     9361 ctgatgtcaa ccccgttttt acaataatat tacatttttt cagattactg tattctatgt
     9421 accggcggtc tcccgccgaa tccgatcact tgctctatta tccgcagttt cggaacattt
     9481 gtcaagccgg aatactgatt tacttttccg tgatttactt ttccgaaaaa gtagcacagg
     9541 cggtctctct gcagtcgctg gctccgcagc ccttgatatc cacatgatcc gccacacact
     9601 tgtaattgct gttataaata cattttaccg cctcacagtc gatactgatg gtatggcttg
     9661 gatgagacag ggaattactg aaagactctc ttccctctct cttctgcgca aagctctcgc
     9721 agcaggtgcc gtcacaatca caggcatgct tacctcccac catgatgtct cccttgctgc
     9781 acagacannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncac aatcacaggc
     9901 atgcttacct cccaccatga tgtctccctt gctgcacaga cagtccttgt tataggtaca
     9961 attttccaca acacatttta aatctgccac gtttccctcg ccttctgccg ctttctgcgg
    10021 cttctcgtct tcttttttga ttttaaaatt ttaaaagagg acacgcggca aaaaagccga
    10081 tgcagtcccg ccacgcgtca tggatagtct gtgcagattt gaaaaactta tgccttctac
    10141 tggatttttt atggaatgtg tgctatattt tcattgttag ttttgactaa ctatttgttt
    10201 ttattcatac acttaataat taaaaaattg taactattca gccccttcac aaaatcgctc
    10261 tttcagagtt tctgaatcat tacaaaatat ttttaacagg aggatttcca atgacatccg
    10321 aagaatataa agcactctcc gtcaaagaat tcaccgaggc cgccaaagtc tacgacagcg
    10381 gtcactccgg catttatgaa atgtgtaaag atgattatcc ttatcttttg gaagaactgg
    10441 aaaaagagcc ctttgactct gttctggact gtggttgcgg caccggtccc gtgatcgaac
    10501 tgttacatga aaaatatccc gacaaacatt acaccggact ggatctgacc ccggaaatga
    10561 tccgcgtagc ccaggccaag aacctgtcga ataccaactt cgtcgtggga gactgcgaga
    10621 acattccatt ccctgaggac accttcgatg caatcatctc ttccaacagc ttccatcact
    10681 atccgaatcc tcaggacttt ttcactaacg cctacagggt tctgaaaaag aacggccgtc
    10741 tgatcctgcg ggactacacc acgaaatcca aagtgcttct atggctgtgc aatcatatcg
    10801 agatgccgtt agcccatctg tgcggtcacg gagatgtcag actgtattcc ggtgccgaat
    10861 ttaaaaagtt tgcggaaact gccggttttg aagtggtttc tttcgaggca caggcaaaaa
    10921 tgcgtgccca tctcgttgct cgtaaaaact aatatcgaaa tactgtttct tgtcatacca
    10981 ttcaaaaaat cgccgtctcc ctttttcttt ttataaggaa acggcgattt tcatattttc
    11041 ctacttgatt ttatccggca atcggcttac ttgctcagcc tcttggttgc gatctcatcc
    11101 tttaacatag ccaggaattc gtctctggat accgtagtgg tgttctgctg tccgtgcaga
    11161 cgatagctta cggtgccgtc ctgtgcttcc tgttttccta agatgatcag gtaagggatt
    11221 tttttcacct gtgcttctct tacacgataa cccagcttct ctgctctctc atcgatgtcc
    11281 gctctgatgt tattgtccag cagataaccg tacagatcat gggcataagc attcagctct
    11341 tcgtcatcgt tcttgaccgg caggatcata gcctgtacgg gagccagcca cagaggcagg
    11401 ttgcccttgg tctcctccag gatataagcc atgaatctgt ccagagatcc caggattgct
    11461 ctgtgcagaa ccacgggagt cttcttctga ccgtccttgt ccacataggt caggttgaat
    11521 tttgcaggca ggcagaaatc cagctgacag gtagacaagg tgtactcatt acctacagcg
    11581 ggctttacgt ttacatccaa cttcggtccg tagaatgcag cttcaccgat ctcctcggtg
    11641 tactcgatgc ccagatcatt cagaacctta cgcagagcat tctccgcatt gttccacatc
    11701 tcatcatcat cgtgatattt taccttgtcc tccggatctc tcagagacag gacacatctg
    11761 taatcggtaa taccgaaatc cttgtaggta ttgaagatca ggtctactac cttggagaac
    11821 tcggactcga tctgctccgg tgttacgaac agatgggcat cattctggca gaaatgtctg
    11881 cctctttcga tacccttcag ggttccgctg gcttcgaaac ggaagtcatg ggcgatctca
    11941 ccgatacgga taggcagatc cttgtaggag tgctgtgtgt tagcgtagat catcatgtgg
    12001 tgagggcagt tcatgggacg cagtacgaag gactctcctt ccatttccat gggagggaac
    12061 atgttctcct tgtagtgatc ccagtgaccg gaagtcttgt acagattcac agtaccgaca
    12121 cagggggtca taacatgcag atagcccagt cttctctctt tatccttgat ataattctcc
    12181 agctcctgcc atacggtgta acccttggga aggaacatgg gaagtccgcg gcctaccaga
    12241 tcatcagtca tgaacagttc catttccttg ccgatctttc tgtggtcacg ctctttggcc
    12301 tcttccagct ctttcagatg tgcttccagt tcctctgcgg tagggaagca gacaccatag
    12361 atacgctgca ttacgtggtt gttggcatcg cccttccaat atactccgga gtgttttacc
    12421 agtttgaagt tcttgcacag cttggtgttg tctacgtgag gtccacggca cagatcggtg
    12481 aaatcaccct gatcgtatac agtgatgttg ccgtcttcca gattgctgat caggtccagc
    12541 ttgtacatat catccttgaa catttccatg gcttctgcct tggaaacttc tcttctgtag
    12601 attttcttgc cttccttaca gatcttcttc atctcttttt cgatggcagc gatcacttca
    12661 tcattgacta catcgttgcc gagatcgata tcataataga atccttcctt taccacaggt
    12721 cctacccaga attttgcatg agggtacaga tgcttgacag cctgtgccat aacatgggca
    12781 caggaatgat tcagagttgt cagtctttcg tcttccttaa aattaaccat ttttgttctc
    12841 ctttttgttg ttttctaaac tattcagcac cttcattcgc aaaatcgcat ctgcgatgct
    12901 actctgaata gtcgcaaaat aaaagtccct tgcaacgatc tgcaagggac gtatcacacg
    12961 cggttccacc ctgattgcaa ttatctaaag ataactgcca ctcatttgct cgtaacggga
    13021 gctgccgggc tgtattaaag ccactccgag acggtcttcg tccgcatccg gcaaagccgc
    13081 tcacagcgcc tgtctccaac agggcggttc tctctggtgc cttcactgct tcctactctt
    13141 ctcttcaacg ttttctgatc ttaaataatc ataaatttct gttgctttag gacttatgtt
    13201 gatataatag accttttcac acggattgta aagaggtatt tatatagctt tccacatttt
    13261 ctctggacat atccagtgcc accgccagtt ctccgtacaa ctggtcctct gcgatccgaa
    13321 aatatctggc atccaccgcc gtctccttgc gtcccgcctc ttctctggcc cgcttgcgca
    13381 ggtagatggt cttgataatc cggatcagat cctggcagtt acaggccttc agacaattct
    13441 tatattcctg ctccagcagt ttgtcattgg caatggtgat cagcgggatc tccggaatct
    13501 gtcggatcag actctccgcc tcctcatgtg caagcacttt ccgcatggaa ccttctgccg
    13561 tatccaccgg aatatatacc gtactggaag acagatacag aggttttaaa atataatact
    13621 ctctcttctt gtccactcct gcaatatcca atgtcgtgat ctcttccacc acgcaaacgc
    13681 ccttactgcc acatactaca cgttcgccct tctgaaacac cttgaccttc ctttcctctg
    13741 cccattgcag aaaattgaaa acacaatttc cgtttactac caatagtata cccatatttt
    13801 aacagaaaaa acgccgaaat gtcagtatct ttcggcgttt ctgattattt tcgtgaatat
    13861 tgctcataag aaatcggtca ggtgttgtgc attatttctg ataggcgatg cgtgggtctc
    13921 catttttcag gtaaataatg cctctgcgtt cgaagccgtt cttctctaat aagtgctgca
    13981 tggtgtgatt gtcaaaatgg gtatccgcac gcaggtaagg tactttcttc tcacagaagg
    14041 caacgcattt tgcaaacagt cccttcactt ccccgtctcc tgccaggcgg tggattgtgc
    14101 cgtaaggagc ctcctccctc cagctgccat cctcaatata ggcataggtc ggttcctctt
    14161 ccagcaaaag catgaatact ccatggatcc ttccgttctt tttatataca tacaaccgct
    14221 gtttctccat atctgtccgc agtgtctccg ggtccggata ggctccattc cactggttcg
    14281 gattcccgtt tgcccgcatg taagccttgg cgatgtcata gannnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnttccactg gttcggattc ccgtttgccc gcatgtaagc
    14461 cttggcgatg tcatagatct ccaggattct gggcagatct gcctcttttg ccgttacgat
    14521 ctcctccatc ccgtcgccat ctttcctctc ttccactgcc tccggtaccg ttttccccag
    14581 aatgtccctt acggcggtct ccatccgctc tgccagctgt ctgtggccct tcgctgtcag
    14641 gtgcacatga tccgccttcg tcagttcaca gcctcccgca tctaagaaat ccgcccgttc
    14701 agccactgcc agaaaagcat attctcttgc cagtgctctc gttgtctcca cgcttttttc
    14761 attaaaatca ggataaatca caccctcttc gatgggaacc ggtgccacga tcaggatctt
    14821 cggtccattc tttgcccagc agggaaccga tttcgcctct tccaagagga tctgcatgcc
    14881 ctttgcaatc ttccgcgcat ccgtattgaa tttcaccttg gaatcattgg tacctaacat
    14941 caggatcagc aggtctaatg gctcgtggct gttcaacagg gcatgcaata ccggcagcag
    15001 attcaccgaa tccatgtccg gatcatcata cacacaggtt ctgccgctta agccttcctc
    15061 aatgaccaga tactccgctc ccaatgcctt ctgtaacagg caggtccagc gttcctcttc
    15121 attatatctg ctgccgtggt cggcactctc cgacggatcg gtgcaaagcc cgtgggtatt
    15181 ggaatctccg atgcacaaaa tatgtttttt cactgtttaa ctctccgttt tctatttcag
    15241 tctgtaacca tccaggatca atacgtcagg cttcaatatt agggaaaaat acaataacat
    15301 catccccctg gcattccaca tacatgccct ccagttccag gttcacatca tccacattgt
    15361 gatcgtcgaa atatttggtc aggttcatga tcgtctccat ataggaatta tctcccttta
    15421 ccaggatccc cagacccttt tccggctcct gctcatagaa ctccagacct tcggtttcat
    15481 tgacatattc acgtattgct tctgctatgg tcatttgatt ttttctcctt ctactgttcc
    15541 tttgttcata tttgctgctt ctctttattg tatcataagc tcacgaattc gtgctatcgc
    15601 actctacatc ctgcttcgca ggatattcgt tcgctaatga gcccagaaaa acaaatgtct
    15661 gcttgcagcc acttgttttt ccttgaggct cattatatca tgattcgcac cttcggtact
    15721 tgacgttgct tcgcaactat catgatcaaa ttcgccgttc gccaatcatg caagcatgtt
    15781 ggctctcttg cgctcattat atcataaaag gcgaaccctg gcaccgccaa agtccgccct
    15841 tcatcatttt cttctcttat tcttcattca tcttagccag cttggctgcc tggtcggctg
    15901 cgatgcaggc atcgatga
//
LOCUS       DAWEJW010000065        15784 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1367_length_15784_cov_63.525017, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000065 DAWEJW010000000
VERSION     DAWEJW010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15784)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15784)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15784
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_15784_cov_63.525017"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4035 a         3930 c         3488 g         4331 t
ORIGIN      
        1 tcccttgcgg atgtggtatt tcactgccgg gatcttggtc ttggtgtaga gcaggttggt
       61 gttgggatct acggtgatca tgtagcctac gggcggcttg tctgccactt cgttaacctg
      121 tgtattttca tccaataccg cactgacagt acaactgcag aattcgttgc tggcctgggc
      181 ggacataccg tccacctttt ccgcaaagcc gacctcttca aaatcacctc tgctgactgc
      241 aaatccacag tcgtaggcca cgcagtcaat ctcgctggtg atcttatgta ctctggtatg
      301 tccaccggca tccggggtga tcgtggtctc taccgtaatg ccgggatagg gactccactt
      361 cgaccatact ccgttctccg tgatctttga ctcctcgcag atccggcgca catagacata
      421 accatcgatc cagaacgcca gcatattgtc cggggcattc tcatgcagct cgtagcagga
      481 ctttgccacg ctgatactga accgggtatc ataagcaaac tttccgtatt ttgccacgat
      541 ctggccgtgt ccgttagggc tgtagacgcc tggtgcaaat gctgtggtgt gattgccgta
      601 atggtagaca aacagatcgg catacttctg gggacaggcc ggtgccagct tcggcaatgg
      661 tgcctcttcc acactccaga agggatgctc atcgggaagc aacaaaaagg caaacacctt
      721 catgccccag taaggagaac cgggggcatt gtaccgctcc gccatggtca gattcggata
      781 tccatagccg atggtcagca catgatctct gtcgaagatc ggacgcttca gccaggtgcg
      841 cagatgcctt gcgatcagac ctttcatcac gggaaccggg aacggctcca gtcctgccag
      901 caggcagaca ctgaaaaagc tgacctgaga gaaacgatag gtcagggatc tgccgaaagg
      961 aatggcctca ccctcttcat cgaaccagta gataaactgt tttgcaaatt ccatggctct
     1021 cgccttgtat ttttttgccc gctccgggtc atctttctcc ataatcacgg catagatcag
     1081 gctgtaaaaa tggatggcaa aggaaatata gtaatccttc tgaccggaat ctccgtcctg
     1141 ataccagcct tcacccagat agaaggtctc cagcccttcc agatattctt ccagcatctc
     1201 ggggctatac cggcgtccca cctttttcat tgcaatgttc accagtacgg cgaacaggat
     1261 ccagttacac accggcagag gatgctcatt gatcttattt aaatagttgc agagattctc
     1321 cttctgtgct tccgtgagag gatcccatac cacttcgggg gcaaacataa tgccatagga
     1381 aatggctgcc atctccacga atctctgatc aaaagaggtg cattctcccc aatattccgg
     1441 gttcttctca tcggttccgg ccgtcaggcc cttgcgatag atctcttcga attccggctc
     1501 ttttccaccg cctgcccaaa agggcaccag tccccataag ggacgggaaa aggcttccat
     1561 ccatgtggca ccgatgtcat aatgtgccat ggtaacgccc aacgtcaatc tcgccttttc
     1621 ttcactgtaa tagggtttta acggctccag gatagataac atacagtcct gaaaatcttt
     1681 tttgctatgt aagttcatgc agttagtccc tccaaaataa caaacgtagt aactatattc
     1741 ctatattaga aatacttctt acagaagccc agacaggatg ccatgccgaa gatgtttgtc
     1801 gtagagtgtg agattttctt gaaaagtggt ctggcaacag ggcgaaagcg aacacgatgt
     1861 tcgcgtcagc cgatcgtcga catggatgtc gactgtaggc gggcccgtca taatgagaaa
     1921 aactaagcca cttttcttta gaaaatccac actcggagac cttacatcaa ggcatgcgta
     1981 tctgtatggg cttcgtatga tatcaattta atataatcta ccagtactct ctccaatcct
     2041 tcaataaccg ggtcagtgcc tccatgtaga agtaatctcc ccaggaattg cactcgtcta
     2101 cgccgtaatg attgcaggta ttgtaaggag aatggtttga gtaggtgctg tgcagcacca
     2161 gtccgttgga ttccttcgga tcctttacgg catagcccac atagacagac cgcatgatct
     2221 gttttgccag tgtaatgtaa tgctctgctt cttccggctc cagatatttc gccatttcca
     2281 gcataccaca agccgcgatg gaagcggaag aagaatctct cggctggtca tccccgtcgg
     2341 tgaattccag atcccagtaa ggtaccagat ctttgggcag atgtgccaga aaatactctg
     2401 ttacatgccg gaacagatca atatattctt ttcttccggt ataacggtaa gccagtgcca
     2461 taccgtagac gccccaggcc tgacctctcg cccaggcgga accgtcacgg tatccctgac
     2521 aggtggcacc atgatccggt gctcccgtct tcatatcaaa aaagaaggta tgccaggtag
     2581 aataatcctc acggatcaca ttggcaacag cggtatgaat atgtttctcc gcaatgtcac
     2641 ggtatttcgg atctccagtc tcatcggatg cccagtacaa cagaggcaga ttcagtaaac
     2701 agtcaatgat caggtggtaa ttctccggtg catccatggg tccccatgcc tgaataaact
     2761 cccctaccgg atgatatctg gtgatgagct ggtctgctgc cttgatggct gcttctctgc
     2821 cctctttgct gccggtcagc ttataagctg ccacgcagga aggggaatac agaaatccca
     2881 tatcgtgatg atccacttcg attttcttgt ctatacgatc caaaaagctc tttacctgaa
     2941 cttccccggc ttttttcagc ctttcatcct gagaatattc ataagccagc cagatctctc
     3001 cagtccagaa cccacaggtc cagtaattgt tcggaatcgg ctgatagaaa ccgttctcgc
     3061 tgtatgcttt ctggaacttc tctgtaaatt ccggcagatc ccggattacc tgctccgtgg
     3121 aaaactgcag agcgtctttg atctccgcat ctgtgatctg cggatatttc ttttcgtcaa
     3181 atgttatcat agtcttatcc tttaccttat tatgcatttt tattatacaa gcctgtgaca
     3241 tggaaccggc acaggcttgt gaaaatacat tgttatctgt tatcccttca gtcccgtaga
     3301 ggcaacaccc tctacaaagt atttctgcaa taccaggaat acgatcagta ccgggatcag
     3361 cgacaatacc gatcctgcca tgatcagacc gtaatcggaa gagtactggc tgatgaacat
     3421 ttttaatccc agctggatgg tcttcttctc ctgactcttc aagtagatca ggggtcccag
     3481 atagtcgttc catgtcgcca cgaaggtgaa aatcgtcagt gtggacaatg ccggcttcga
     3541 cagtggcagc atgatgctgg catagatccg gtactcactc ataccgtcga ttctggcggc
     3601 ttcacacaga tcgtcgggaa tgccttcata gaactgcttc atcatgaata caccgaatgc
     3661 ggaaaacgcc tgcaggcaga tcatagcaag cagcttatcg ttcagtccca ttcttctcat
     3721 catgatgaac tgaggtacca tgtatacctg ccagggcatt gcgatggttg cgatgtatgc
     3781 caggaacagt ccgttcttat gacggaagtc cagttttgca aaagagtatg ctgcaaagct
     3841 gctagtcagt aactgtaaga aagtaacgat caatgtcaag tataccgtat ttcccacgaa
     3901 tctgccaaag ggaatcttcg tccagatctt cacgtaattg tcccacttgg ggttcgcagg
     3961 gatccataca aaaggattca tctggaatac ttctctgtca ctcttgatgg atgcggaaag
     4021 catccacagg aaaggaatga tcatgaccac tgcgatcaga atgagaatcg catatacgat
     4081 cactttgcca aaaacggctt ttttatgtgc tgatttcata atctgtgcgc ctcctaacgt
     4141 tttaccctgc cgtacacttc aatctgactc agtgccggga agggggatgg atcttctgct
     4201 ttgatcaggt tgcaaagctc cagccaggtg atctctttct cggggatcgg cagtacatgt
     4261 gccctgctgc tcttctctaa gggaaattcc tgttcactgc catcggaaaa ccggatcgtt
     4321 acctgctgcc accagttgtc atgcgggaaa tcggatctgg tgtacaacac gatcctgtct
     4381 gccagtaccg gtctgccgaa gtccagcttc atagctgcat catcctgcat gttgattccc
     4441 caggattcgt agggccattc tccgtgggag cggttcgccc ggacaccgtc aatggcgttt
     4501 ttcgctgcaa atacgctctc gcctctggtc tccacattgg ctgtcgcatg cggataacag
     4561 ggaacatcca tatgttggtc tgcaggattt agtgccaggt ttctatagac tgtgatctca
     4621 tcttctcttg cgacttccgc atagagataa tgtttatttc cggaaaatac cttaggggac
     4681 atgctgatcc ttttttcccc gaagggaaca taataggata cattgtcggt tatgtatacg
     4741 aaagcatccc caagagcatc atccacctgt aaatgtacat gtatattttt ctcatcggtc
     4801 tccagcacga tccggtcacc ctcttcatag gtatgggtgc agaccagatc cacaaaatcc
     4861 tcaccgctgc tgacgcagat ggtgttgctg tccttatcga gaaccttcaa agccaagatt
     4921 gccattatgc tttctccctc tttgcctgac cgcggaactg aatgatcgtt acgaacagta
     4981 ccatcaggaa cagtaccatt gctacagagc tggagtagcc cagatcccag tcaataaatg
     5041 ctttctgata gatgtagtat acaagaacgg ttgctgaagt ggttaactct ccgcttccac
     5101 cgcctgccag catgatggcg atatcgtaca ccttgaagca gttgatggtc agcatgacga
     5161 ctacgaagaa ggtcgtggaa gacaactgcg gccaggtcac ataacggaat ttctgccagg
     5221 tatttgcacc gtccagactg gctgcctcat acagttcaga ggagattccc tgtaagcctg
     5281 ccaggtagat taccatataa taacccatat atttccatac actgaacaga atcatggaga
     5341 acagtaccag tccgccggtg aaccactggg gaagctgatc ctgcggtaca ttgaaaacat
     5401 tcagcagaat gttgttcaca ggtcctttgg aggggtggaa cagcattttc catactgcgg
     5461 tgattgctac cagagaagct acatatggga aaaaggaaag ggttctgaaa atacctcttc
     5521 ctattacttt ctgatttaac acgatagcca gtgccagaga tgcgatcatg gtcagaggca
     5581 cggtaccgat acaatagata atggtgtttt taaatgctgc gataaagaag gtatctttcc
     5641 acagtcttgt aaaattactg atgccggccc actggatcgg gttactcccg tcccacttca
     5701 tgaaagcaag ggcaaaggca aaaataatgg ggatcaaagt aaatacgcag aaaccgataa
     5761 agttaggtgc aataaaggag taggccacca gatctctttt ggtttgacgg cttaatttcc
     5821 gcataacaat tctcctttca aacgcttttt gtgtcatctt ttgcgtcttt ttctgataag
     5881 aaaggggccg taggtacgac cccttaagtc ttattcaaca ttcaacaaac attcaatact
     5941 tttatagaga agatcttcat tactgagcct gaaggtcagc taccgcttta ttcattgctg
     6001 caataccatc atcaatgctc atctcaccag tcatgatact tccatgatag gaatccagag
     6061 cggagttgat ctcagataca ttgggagcgt aaggaacttc cagatacagg ttggatacag
     6121 tcagagcctc cttgctggct tcatcggtgg ggaatccttc cagaccggag atcatgtcta
     6181 ctacttcatc agtcatcatt gcagggaagt taccactgga agccataact gctgcgcctt
     6241 cttcaccgct tacccattta acgaaatccc aggacgcatc gggagtatca cttgcagtgg
     6301 ttacagccag accggtgatg gtacccagag tagaaccggg ctctacgcct tctgcatggg
     6361 gatacttaac gataccccag ttaccgcaca ggctggaatc atattcgccg gacttcaggt
     6421 tggacatcat ggttgcgatg aaccaggagc ccatgttcat cattgctacg tctccaccgg
     6481 agaatgctgc ggaataatgt aaaccttcgg tcttcagatc cgtgtacttt ctgcatacac
     6541 cgtcagcttc ctgattcaga accatctcat agtaaggttt gaagaaatca tagtttccat
     6601 ccaaaatagt atgcttacca tccagaacac cgaacagctg aactgcactt ctccatgtgt
     6661 gataatgagc accgtatacc tgagagccga aggtggtatc ggtcatctct cttgccagag
     6721 catcgtactc atcaaaggtc atatcgttgg taggataagc aacacccttg gcatcaaaca
     6781 gatccttgtt atagaacaat acccagaagt catttctgaa aggcagctca tacaggcttc
     6841 cgtctacagt tacctgatcg gtaacacctg cgtatttgct taaatccacg ccatctttgg
     6901 agatgtaatc atccagggaa agaatagatc ccttctgtac cagcgttgca tatccgggta
     6961 catccttgac ggttactaca tcgaaatcgg agccgctgcc ggaaagctca gttgccagaa
     7021 cggtcatgta atcggtagaa ccaaggtcta ccatctcaat ggttacaccg ggatgagatg
     7081 cttcatatgc gtccttaatg tcaccccagt actgtgtggt ctcctgatcc cagatagccc
     7141 actttaaagt aacttcttct gcgggtgctg cttccgtgga agctgctgca gcctcagtgg
     7201 ttgctgctgc gggtgcctca ccggtggaag ctgctgtgct gccgttatcg gagctgccgc
     7261 agcctgcaag catggtggtc atcattgctg ctgtcagtac taaacttaat actttctttt
     7321 tcatgtaaac cctcctgtta atttgatact atgttacgtc tgttacgaat ccgttgttat
     7381 cggttccttg actgtaacta aagtataacg aaaaagatct ttccgtaaca tggaaaaatt
     7441 tataatataa ttcacattct tttataattt ttttatcttt tttctaaaag tgctttcgga
     7501 agatgtaata ttttatttta atatgcactt tttttagcat cttgtttttt caactaccgt
     7561 ttttctttcg ttatgttata ctgaaagcat gaaaaagact gtttatcact caaaaagcat
     7621 acgaaatcgc atattgctct tcactctgag cgccattttg ggaatgtgtc tgttgatcag
     7681 cctgttcagt tattacatat tccggcacta tctgcagaac actctgatcc agtccaccga
     7741 gaccagcctg cgactgttgt cggaatccat ggacaacagt attgatgaag tgtaccggtt
     7801 ggtcagatat tgccagacag attccaatat tgccaattat attgaacata accccaatcc
     7861 cggctctgtc ctgtccgtat ccacttatga ttccttctac gaggaatgca gccggaattc
     7921 ttcttataat tatatgccca gaattgccat tgtctcacag gagcattatc tgcaggtggt
     7981 aaccgcaacg tactcttcta ccgcagatct tgcaacgctg attccggagc ttccttatta
     8041 tgaagaatta ctgactgccg atgattatga tttttcgccc ggtattgtat ccgatccttt
     8101 tcaccgtgcg ggacgtaaag tattacctgt gatccgtccc attacctacc aatacaataa
     8161 caggcaggca ggatatctgt tcatcgaaat atccgcagat ctgttcacca tacctttgaa
     8221 ggattacagt tgtccgtcag acagctttct ctatctgtcc attgcaggac atacctatat
     8281 ttatgaaaac ggaggcttgc aggaatttac gcccgaatat agtattctgg atgacctgag
     8341 cacatattct ctcacgggca ccacacagat ttccaaaatc cacagtgcta ccatgggaga
     8401 acagattctg gtcaccactc cgttaaatat gccggactgc tacttaagtc agagtatttc
     8461 acatgcggag cggaccaatc aggcggtttt gttcatggga atccttgcct gcatcctgat
     8521 cggcattttg tctatcggtg tcgttctgat gctgttgatg aatcgtatga tcacggtacc
     8581 tgtatccagg ctgcagacac ggatggtacg catcgccggc ggagacttct ccagagaccc
     8641 ttctgtggaa tgggatcacg aacttgggga tatcggacgg ggaatcaatg atctgtcgga
     8701 aaatgtatcg gaacttatgg agaaacgact ggaagatgag aaacagaaac aggatctgga
     8761 atacaagatg ctgcaaagcc agatcaatcc tcatttcctg tataatacac tgaactctat
     8821 caaatggatg gcaaccatac agggggcaac cggtatctcc gagatgacca cctccctgtc
     8881 ccgtctgtta aaaagtattt ccaaaggcac cagcctgttg atcgatatcc gggaagaatt
     8941 atctctgttg gagaactatt tcaccattca gagctaccgc tacggcggca ccattaccat
     9001 ggacattcag gtggataatg aatctctgta caacagtgag attatcaagt ttacattgca
     9061 gcctctggtg gaaaatgcat ttttccacgg tatcgaaccg aagggctgtg ccggacacat
     9121 ccggatacat gtcggctatg agacatcgga caacaccgaa aagatccgta ttgatgtcac
     9181 agatgacggt gtaggcatga cagcggataa agctgcacag gtattacgct ccaacgacga
     9241 cagttccgcg gatttcttcc gggaaatcgg tgtcagcaat gtgcataaac gcttacaata
     9301 ccaatttggt gcagaatatg gcattaccat agaaagcaaa gaaggggaat atacgactat
     9361 gtccatacac ataccgaata ttcctctgca taacaatgaa actgtttcat ccgaaactat
     9421 ttcatctgaa aacacttaat ccggacagga agctgtccag aatggagtta ctatgtatca
     9481 gcttttaatt gtggatgacg aacctctggt tcaggctgga atccgttcca tgctgaactg
     9541 gaacgaaatg aatatcgaga tctgtggaac tgccatgaac ggtcaggcgg cgctgaagat
     9601 tattgaagaa aaatctactg acatcgtgat cacggacatt aaaatgccgg tcatgaacgg
     9661 tctggatctt gcgaaaacat gcagagaacg ctatggggat aactgccctt attttatcat
     9721 cctcaccagc tatgaagact tccagatggc aagagatgcc ttgtcttatc aggtatccga
     9781 ttatctggta aagctggaac tgacaccgga agtactgaaa aatgccatag gccgggtgct
     9841 cacacagatt tcccaatctc ggaaaaagca gctgtccgcc gtaaatattc atccttttta
     9901 cgacaaattc ctgatcagtc tcctgcatga cctgttcgag tccgaagaac agttccgtct
     9961 gcagggcaga gatctgaatc tgaatttcga ttacggtgct tatgtgtgct gttacggaga
    10021 gattatcagc cctcaggccg accagatgtc tgccgaaaaa cagatgcctc tgttcaccag
    10081 ttcgttacag atgatacggg aactgggggc taaatatctg cctctttatg cgttgtctct
    10141 ggatctgcgc cattttgcac tgattttctg ctttgcggaa tcctccgata ccgatgatta
    10201 tgtggaaagt ctgacagaca ttctgcaaag catcagcgga acactgcaga attactataa
    10261 tgtaacactg cgttgcggca tcggcatccc ggttcagact cccgctacca tctgtgactc
    10321 ctaccagtac gcgagacaga tcttccggaa tacggaaccc aaggacgcaa ttatagcttt
    10381 cgataccgat cattcgcagg aaaacgccaa gaattctttt aacatcagcc tgtttaaaaa
    10441 tgatctcacc agagcctttg aagagtatga tccggatatt ctgcagacta caattcagtc
    10501 cctctgcgat ctgttcaagg atcatccggg acattatgta caggctctgg acgctgccag
    10561 caatattctc tatctgtcga tttcgctttt acaggacggg gaatctattg tgtccggctt
    10621 ttttgcggga gatccggatg gctaccgttc cctctataag cagtccaatg tggatcacgt
    10681 gatccagtgg ctgcaattct tctgcggaca gctgtgcgaa ttgttccaat cccgtcgaaa
    10741 ggattataaa aaccacatcg taactaacgt ccggaaatat attaatgaac atgttagcga
    10801 gcggttgtcc ctgaatgaag tagcagcggt attcggcatc agcccgaatt acttaagcca
    10861 gctgttcagc aaatacaacg ataccggctt cagtgaatat atcaacatct gcaagatcac
    10921 ggaagccaaa cggctgctgg aagaagaaaa tatgaaggtc tacgaggccg cagaggctct
    10981 gggcttcgag agtgccttct atttcagcaa ggtatttaaa cgggtggaag gtgtctcccc
    11041 cacggaatac gtcaacaata agttcagtta actgctatta cggtttgtct cttattggga
    11101 attatgaata tggaaaggca ggatcagaga aatgaattca caaccgataa gtatagaaaa
    11161 gcgttttttg gagacagcaa atgcattcca cggaaacagc cacccttttc acccatttcc
    11221 gaaggctgtg gaccgaaagg cttatgaagg actcccggca gcattgaagg aacttctgat
    11281 tcaggccggt gaggcaaagc tgggatatga attcccggtg atacatgcca cagattacat
    11341 gcgttttaaa agagacggtg accgggctgc ttttgaagcc ctctattttg ccaaacgtaa
    11401 tgccttaaat gatctgatcc aggcagaatg tgtagaacat cagggacgtt ttctggatga
    11461 tattctcaac ggtatttatt ctatctgcga agagactgcc tggcagcttc ccgcgcataa
    11521 ctcctatatc cgcgataccc cgcagttgat cctgccggat gtgaccaggc ccgtcatgga
    11581 tctgttcgcc tgcgagaccg gagctttgct tgcctgtgcg gcttatctgc tggaagacga
    11641 tttgaatgcg gtcagcccct atatcctgac ctgcatagag gacaatctga agcaccggat
    11701 cctgctgccc tacctgaccg cacatttctg gtggatggga catgacgatg agcctatgtg
    11761 caactggacc gtatggtgta cacagaatgt actgctgacc acgttcctga tgccctggag
    11821 tgtggaaatg tcctctcggt tgtcctctcc ggtgcgcaca ttctgcggaa atgctccgtt
    11881 gttcctgccg gagaatacgt ctgacacagt tgtcacattg caggcgattc tgcataaggc
    11941 cgctgaaagc tgtgattatt ttctcaagga ttacggcaat gacggctgtt gcggggaggg
    12001 tgcccaatat tatcgccatg cgggactatg tctatacggt gccatgactg tgctgaacac
    12061 ggttaccgac ggtcatttcg acactctgtt ccggtgggat aaggtaaaaa atattgcatc
    12121 ctatattctg aacgtgcatg tgaatgataa atactatttt aattttgctg actgctctcc
    12181 tatagccggc cgtgccgggg tacgggaata tctctttggc aaggccaccg ggcaggagga
    12241 tctctgcctg ttcgccgcaa aagatttcca ggcgggacag ggtcagcttg tcacagatga
    12301 agtgaacggt gggaatctgt tctatcgtat gcagaccgta tttcattatg aggaattgat
    12361 gaaacaggac acttccggaa cgctttccca ccgggatgtc tattatccca gcgtggggct
    12421 gttcctcgtg cattccgata ctctggatct ggcagtcaaa gccggagaca atgctgacag
    12481 tcacaaccat aatgataccg gcagcatcac cttatacaaa aatggactgc ccgttctggc
    12541 ggatatcggt gtggagacct acacccggaa gaccttctct ccaagacgtt acgagatctg
    12601 gaccatgcag tccggctatc ataatctgcc tacgatctgc ggcacggatc agaaggacgg
    12661 tgcggaatac cgtgcggaaa aagtggtgac gaagctgacc ggagccgaac cttctatttc
    12721 catggaactg gctgccgcct atcctgccgc acaggctatt gtgccggatc tgacttatgt
    12781 ccgcagggtg acattaaaaa agtcttccaa cactgtcgta ttggaagacc atacaaatgc
    12841 acaagatgtt attcttaatt ttattactta tgaaaaacct gttattcttt ctgacggtaa
    12901 actctccatc ggagaagcat ctgcctcctt cgaaggggct gatctgcttg ccatagaaac
    12961 tttaccgatc acggacgcac gcctgaaaac ggcatgggat catgatctgt accggattcg
    13021 gctgaagggc aacgatgcca gcttccggct gactattcaa taaagcctaa aatttcaggc
    13081 ttttctcacg gaatgtttat tcttgtacat ttcatcatct gcccgtttta tggtttcttc
    13141 cagagtttct gctgtcttgc gaaaggctcc gccgattgca agacggcagt aaaactctgc
    13201 tgttacatcc gataaatttc ctttctcatc tccacgcatg gtacttacca gttcctgtac
    13261 tttttcttcc gtacaatctc tgatcagcat tacaaattca tctccgccga tacggaatcc
    13321 gcttgcttgc tccgtgagat acctccggat aaattccgct gctttcagga tcacagcatc
    13381 gcctttttca tgtccgaagg tatcattcat gcgtttcaaa ttgtcaatgt cgaaataaat
    13441 tacaccgatg gtacagtcat tctgcagttc ttttaactgt tctttgtagt gtctcttgtt
    13501 gtagagctgc gtcatgctgt catgattgct gttccaatcg gcaatagcca gctgggaact
    13561 ggacgcaatg atatcatgct ccattttctt catggattca cacagccgtt ccagttcatc
    13621 tccggttctg atgttcatcc ggctgatggg agattcctta ccatcccgca aaatggcaat
    13681 agaatagctg gaagccgcct tggacatggc atttaacggt cccagcacat tgtgctgcat
    13741 aaaccagatc ataaagactc cgaaaagcac cgtaatcgat gtaacaatga tggtaatata
    13801 ccctaaataa gtcaattgct cccttctgac attttctgtg gaaattccta cgataatata
    13861 agccaccgga atattgtatg aggaattcag cggaatgtag taatcgcttc ttccgttata
    13921 ctctaacggt tctattttct cacatgcgat caactcgtct tttatagcat tctgtacctc
    13981 atcatagggg cggatatctc ctaaaaatgc tccgttttct gtcagatctg tgtcatacac
    14041 ataacagcct ccaccatccc tgaaattgac cacgctgatc tgcaatacat tttcactggt
    14101 gttgcgatag tcaatgagtt tattccatac ctcgtaatac gccgtatttc tccgtctgtt
    14161 gtccagatag cccgttacgc tgtctccgtc taccaccagt ctgcaggtat tcgccacagt
    14221 ctgcgccaga gaatctgtgt aattccgatt gacattcata taattgacgt agtttacaaa
    14281 tatgaagctc acgtccataa tgcatacgat gataacaata gccagaacga ttttatactt
    14341 taatgatttt ttcattcgta atcctcggaa atcaatactt tctctgatct atatagtcgg
    14401 ctgcattttc ataggtatag atgttttcct cagtgttata gatcttgtcc acctctctgc
    14461 cggcttccag ttcttctatg accgtctcca cagtggatgc cagcagcgga ttacactcca
    14521 cggtggcatg taatttcccc gccatcatat tttcaaagct ttcgtgcaaa gcgtcaaagg
    14581 agatcataat gatgtctccc tcaggcccat agctcttgcc gttccggtcc agtgctttca
    14641 tggcaccaaa gatcatgtta tcattttccg aaatgataac atcaatatcc tttaccgttc
    14701 ctttgctgag cagattttcc atataggtct ggccttctcc ctgagtgaaa ttggcacatc
    14761 ccctgtctac gatagtccag ttcggatgtt cctccactcc atgtaaaata ccgttcgttc
    14821 ttccgatggc agcggtagct ccttcagttc cttccagcag gacaatgtgg attgtctcgt
    14881 tttcccgtcc ctgctctgtt aaatactgat ccagccacgt tacagctttc cggccttcct
    14941 cttcaaaatc cgaaccgatc caggacacgt attccgtttc atccacagcg attttacgat
    15001 ccgctacgat gaccggtatc ccagcttcct ctgcggcatg tatggcatcc tcccatccgg
    15061 tctccacgat gggctgcagg ataatataat ctactttctg ctggatcaga tcgtacatgg
    15121 cttttacctg gcgctccgaa ctggaattgc cgtcgatata gatcagatcg tagcctcttt
    15181 ctttggtgaa cgtattcaga taatcattgg tattggcatc tctccagtca gactcttttc
    15241 ctgtctggat catccccacc cggatgagac cgtcattata ccccggtgcc gcaccccgga
    15301 tcagttgctg cgttttgtcg gctcgttcag tcccacatcc gatcaacccc agacaaaaca
    15361 tcagcacaca agtcacggca actattcttt ttttcatgta accacccctg gttctttatt
    15421 tatcgttatt tttaccgtta tcttctcttt tccaaatcat gcctcttacg ttttatagta
    15481 ccatcttcct gtatagggtg caagccatca ccggtggtcc tcaaaataat tttacgcttt
    15541 ttttatcttt ttaacaaggt ctacctatcg gatactgatc atcagtatcg tcatgatgat
    15601 cctgttcgtg ggatccaatc tatatccgag ccggatcaat tctcaacagc tggaagctac
    15661 catgtatatg aaccaatacc gtctgggttc caaaacgctg accactgccg tacagcctta
    15721 tgccgtcacc ggagataaga cctattacaa caatatgaag gaattacacg aggacaagaa
    15781 ttgt
//
LOCUS       DAWEJW010000066        15712 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1380_length_15712_cov_59.278981, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000066 DAWEJW010000000
VERSION     DAWEJW010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15712)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15712)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15712
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_15712_cov_59.278981"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9460..9559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13666..13765
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3685 a         3924 c         2962 g         4941 t
ORIGIN      
        1 atgccgagac cggaaatact gctgaccgac gcgtgacttg gcaccaggaa aaagtagact
       61 gctgccgcga tgatggccac cgccacagtc aggatccctg tctccttcat tatttctgca
      121 taatttattt ttttcttcat aataacattt tctccaacga tatattttaa tttgtttcta
      181 tactcttctc atcttattat tcttagagta ctattacagc ggcatcaagt ccctctattt
      241 ttccatcaaa gggattctct ctaagtaaat catatttttg cgcatactca ttaaacattc
      301 ttgcgctgct gctgcagttg aagatcaaag cgagtgtctc ttctccattt tttctggtca
      361 ggacgatcgt ctcttcctcg tcccttgcag ctagttccat cggttcaccc tctacgatac
      421 atgcatggtt ttttcgcacc ttcagcagct gcttatacca ttcaaacatc tctttatcct
      481 ggtattcttc atcccagtac ataccacgtc tgcaatccgg atcatttgct ccgggcatcg
      541 caaattcatc tccgtaataa atcatgggca ttcccggtaa taacaattga aaagctgctg
      601 ccaaatgctg ctttttctta ttatccttac atagatgtaa aaatcttgct gtatcatggc
      661 tgtcgatcag attccacatg agaggatagc attttttgtt taagcgcccc ttcaaatatc
      721 caagattctg tataaactgg ctgacattga ttttttcatc tgcaagcaga tctatgagat
      781 tcaggtaaaa cggataattc atgaccgtat cccattcatc tccctcaaga aaatctcctg
      841 cataatgcca gatttctcca atgatcagca gatccttttt tactgcctta acagcctttc
      901 taaatcgctt ccagaagaaa tgactgattt cgtcacctac atccattctc catccatcga
      961 tatcgcattc cttaatccaa taacatgcaa catctgtaac gaatttttca acctccggat
     1021 ttttcagatt taactttggc attccgccat aataaccaaa acatttgaag ttaggagttt
     1081 ctcctttttc acccttcagc ggaaatctct cgataaaata ccagtccaga tatctggatt
     1141 tatcttcttt ttctataata tccttaaatg caaaaaaatc tcttcctgta tggttgaata
     1201 ccgcatccat cactactttc attccacact catgcgcttt ttgcaccagc tccttaagat
     1261 cctctgttgt tccgaaggaa ggatcaatct ggtaataatc agtggtatca tatttatggc
     1321 aggaatccga tttaaaaata ggtgttaaat acacaacatc tattcccagc ttctgaatat
     1381 catccaaatg atccataatt cctctgagat cgccatgcag attatcctta gatgtaatgg
     1441 gtgctttata ccacagttcc ttgtctattg gctgtgatgc tgcaaagcga gatggaaaaa
     1501 tctgatacac aaccttgttt gctgcccact ccggcacctc aaacatttct tcctcacgaa
     1561 ggttttgcgg acagtcgaac attctgtccc tgtttgtaat gcattccctg ctaaactcat
     1621 agttgccgta atataccttt tctccctgca tatccgtaaa ttcaaaataa taacggaggc
     1681 atattaaatg catctgaagc tgtgcctcat aataatcatg aaactgtgat gtggcaacct
     1741 tcttcatcga aatcgtcttt cgtgtatcct tccatcccaa tggtatgtac ttgtcctcat
     1801 aatgaagaat gatctctttt atatcatctt tttttacctg cacacgtatc acaaataaat
     1861 ccttatcgat tgcatagcag aatcttttat ccatatcatg aagaattgct gaatattcca
     1921 taattttttc ctttctatag ctcgaaaaaa gcgttttttc cttcttgatt ataattcctt
     1981 tttattatgc tgcacaagtc acaaaacgat aaaaaggtca ccgtttctgc ctgttctgtt
     2041 ggggcaacgg aggatttcat tgcgcattct ccttctgaaa tttgtgttcg taactgtcga
     2101 aatttacaga aatatgcatt ttatcagtta tatcttataa ttttagaaat atgttgtctc
     2161 tttggatgag ttcgacatga gcagaaacgg gatcaagcac cgcaatatct gcgattttct
     2221 gttggcagag gaatggaatt aaaatctctg cgaatctcca tctgaaagtc tcactgtaat
     2281 agattttttc ttcttcaact gtaaaaggtg ggcgcttacg ctcccacctt cacactttta
     2341 ttcaattact atgtcagata cttattcttc tgaaccactc tttttcctta catagaatat
     2401 ggctccaata acaatcagca taatacagat acctataata atgattgatt aatatttcta
     2461 ttagattact accattatat cgtgtgatag gtcggatatc gataaatttt tgaccaaatt
     2521 tttttgccga acatccatta ttaccagtca atcacatcga aaaattcttc tatattgcta
     2581 ccctttttat taataaaatt cacatcaaaa aaagtatctt taatcacaat atcaaaattt
     2641 ttctttttta tctcatattg attgtacagc agcttttctt ttcctcttaa tgattccatc
     2701 attatctgat atgacaattc tcctgaatta aggaagttat aaacatctgc atttccttta
     2761 aaagccgcaa tactttttgc tcccagaaca gattcaataa atttatgtgg atcctgtcct
     2821 ctatactgag gactgtgtaa gcatgccaca tactcaaaat ccggattgtc cataataata
     2881 aaatgtggtg tatttccctt tgcattctga atcctgcagt actccaacag ttttttaaat
     2941 ccatccagtt ctcccgggac agtagctatt ctatctgcat ccataataat gaattttgcc
     3001 agataattac tctgtgactc cgtttttatc ttctgaagga agtttgaata gcccccacca
     3061 tgcatattga tgggttttaa aaccagttct acaccctgat tctttctcat cttgtctata
     3121 taattatatt ctgtatctcc ctcacagaat actgcaaacc tcttctggag gactcttgtt
     3181 tttctactca aatgccgtac ctcccaatat tcccttcata taaaactcat atatcttagt
     3241 gttttcatac ctcacttccg gaaaatctgc tagagaatac aattctgatg tcaattcatc
     3301 tttacttttt gtaatgaaat aaatctgctc tttcatgtaa gtctttaaat tcaagtgcaa
     3361 tacattatgt gtagaaaata caaactgccc tctgtgctcc ataccgttaa tatacgcaat
     3421 aacacggtct gacaagattg gattaagaac ccggtccatc tcatctgcaa ataccacttt
     3481 attttcatat accacgcgaa atatctgtac agcccatgca aaaaattctc tgacaccggt
     3541 tgaatcctgt gataattctc tcttaaactg ctttccctgt gcattcttac gaattaccag
     3601 agttttagaa taaggcttct catcatccac ttcaattccg caaatgctgt aatctaccat
     3661 cttaaaaatt tctacatatc ttggatcttt cataatttca agatcccgtt cctgcctgac
     3721 aatgctgtct gtttgatcag aaggtatatt acccggatat aggacattta acatccattc
     3781 aacagttgct gacgcatcct catttccgag aagagccagt ttaactacaa ataatccatt
     3841 actattattg ctattcttaa gagcagctgt cacttccctg gctgttcctc ttcgtaatgt
     3901 atatatgttc tcctgatccc gtaaaatatc aaacactaca tgctcagctg atttccgctg
     3961 gctgcttctg ataagttttt cgctatagat tccttttgta tcaacaatag tttcatactc
     4021 ataaatataa tgattccccg ctaagaatac tacattaaat ttctgtttgg gattattaca
     4081 ctccatcaaa gtatctgtgt taatatatga attaacagac tgcactttca tgtttgtatc
     4141 aaacaatgat ttcagatatt ttaatccatt tataaagttg gacttaccac ctgcgttctc
     4201 acctacgata actgctgttt tcagcaggtc atatcctgtt tctgttgtag tatagttatc
     4261 cggaaagcgc ttttttactt tcccgcctgg tgcttctaat gaaaactcgc actcttcctt
     4321 gaacgaacaa aaattgtcaa acatatatct tatgagcata cttccaccgc ctttcaataa
     4381 tcattatata ctaaaaagag cctatgtaaa ccagtttttt gttttacatc ggctcttttt
     4441 atggatatca tgttaaaaag cgagcaccaa atattaaata cattctttta gctgtttttt
     4501 gttgattttg ctctttttat tactcctatg aaatcatcat tattcttcct ctgaaccact
     4561 ctttttcctt acatagaata tggctccaat aacaatcagc ataatacaga tacctataat
     4621 aatgattatc gtattattcc atgtatattt tgaagtgctg tcatctgtct tagacgcagg
     4681 aatggtttca tcagctttgt tgtctgcctg accgttatca ctcataatct tggcatcaac
     4741 aacaatcgtg taatctgatg catgggtaaa tacaagttcc acatttccat ccggatcaat
     4801 ctgtccggca ctgataaatt ccagttctcc ggtctgttca ttgtagtaga acaggttcgc
     4861 atacagtcct gcattctttg attccatgtt gacggtcagt gtggctgtaa atccaaactc
     4921 tccgtcataa gcaagcgtca ggttcatgga agaacgttcc cctgtcacgt tgttgatcac
     4981 atccacagga atggattttc cggcgtcggc tccgactgtc acatcaaaat caatatctcc
     5041 agctgcatcc gtaatatcct ttccataaat cttccaggaa agaccatttc ccatatccag
     5101 aacaagtgtt gtatctttgc ccttgatact gtcaattaca tctttcggta ctacagttgt
     5161 tccgttcatt gcaaccgtta ccgtatcgcc tgccttagct tcttcaagct gtggcttgat
     5221 aacatcccag ccttccttgc cgctgtcgtc cttgatgtaa ggcttaattg aagtactatc
     5281 gggctgtggc ttctgattct gattgccatt gtctgtgcca gactgcggtt tctggttttg
     5341 gctgccatta tccttgctgc tgtcatcgga cttaatctta gtgatcggct gtgtgtaact
     5401 gtgtccgcac ttggagcagg tgtaggtcat gatgccttcc tctgttgtcg ttgcagcctt
     5461 cgtcactttt gatacatagc tgtgtcctgt tgccggcata ttctccgtgt agctgtctcc
     5521 acaagaacag gtatacgtcc ttacccctgc ttctgtacag gttgcctctt tcgtaatgct
     5581 tcccgtatag ttgtgagtgt gcttttcctc cggcagcttc gcaatactct ctgtgtagct
     5641 gctgttgcat ctggtgcagg tgtaggtcct gactccttcc tcggttgtcg ttggctgctt
     5701 tgttacggtt gctttataat catgtcccaa tgctgccagc tcttttccgg tactcagaag
     5761 tttatcacag tccgtacaat aggtatctcc tgcgtatccg gtctgcgtac aggttgcttc
     5821 ctttcggttc ttcacggttg tatgcacatg gttcgtggca tccacttcac caaaggtttc
     5881 actacaaaca ctgcatactg cttttgatac acaggtagcc ggtgttccga ccttgtgcgg
     5941 ttttttctcg attgttgtac cggaggcaag tttttcacca caatccttac agtaagtatc
     6001 tccggtatag cccttctctt tgcaggttgc ttcccgctga ttccgtattt cagtatgtgt
     6061 gtgatcacat tcgcttcttg taatcgtaat gcttacactt tctgtctcgt agttgccctt
     6121 gtcagttcct gtataaaatg cattcgctgt cactgccaca ccatctgcaa gcgccgtgtc
     6181 cctatctgct tcctgccagc tccagccatc cggaagcgta atatcgccaa ccttctttgt
     6241 gctgtgcgcc ggagccattg cattttccgg catgtttgga gctttcgctg cctttgctat
     6301 cgtaaatacg gccggattac ttgtaagtcc tgcatagtta tctgtttctg caacataagc
     6361 ctttaccgca taggttcctg catttacagg tatggcatct gtatatgcgg aatcggctgc
     6421 aggtgcatta ccatctgaat cggcttttgc atacttataa gttactgtac cgaattttgc
     6481 tgttgcattt ggttctggtg tctcatcctt cgcataagta taatcagaac agctaagttc
     6541 tgtagtaaag ctattagctg cctgttttac tgtaagaata ccactcttgc catctgaacc
     6601 gtttccaata aaggatactt cataattggc actatctgat gagcctgtaa ttgcataggt
     6661 tccaacatct gaattgtctt tcgcagtagt tgataagctg acaccaagtg cttcttcggt
     6721 atcacctgct accaaaacat tgtctaagta ggcatcacct gaggcaagac tccatgtaag
     6781 tgcagggttt gtctctccat agaatttttc tttatcagca atcactacgg taatctgctt
     6841 cttggaaact tccggtgtca cttcacaggt cacagaatca taagtatctg catcacttcc
     6901 tgtcggtgta aagacaagcg agtatttctc acttgtgccg actgttggaa gcttatctgc
     6961 atcctcagct gccagagtcc atgtaccctc aaccttcgtg tcactgtttc gtcctgctgt
     7021 cacaactgcc tttgtcatat ctgccttttc aaacatggca gataccgtat taccataagt
     7081 accggaaaga accggtgcag tctttacata cggagtcggc tttgcagtgg taaattcaat
     7141 agcaggctcc gttaatgccg ttatattgcc tgccttgtca atggctgcta tccagactgc
     7201 agttttctta cttggataca atggaaaata aacgtccggc tttccaaaat tacctatttc
     7261 aattttatta tcaccttttg ttcctgtcgt tttgaagatt ttaggtgtcc aactcgcaac
     7321 aaagttttgg ttagcattac tctccttaag tccctcctta cgaacttccc attgtccaca
     7381 ataaagattt tcgtccggtt taaaattcca cccattgcgg ggtgcccatt ttccatcgtt
     7441 ttcgacagcg aactgtgggt actttggctc acctttcata taattttcaa tatctctttt
     7501 gcagtcgtcg taggtgtagt cagtaacccc gtcaaatact ccttcggata caaagaacca
     7561 catcagcgtt gcatcctcgc ttaaattaac cttgagtatc gcctctgtat ccttcagggt
     7621 accgtcttct ttcttcggct cagttactgt cacgacagga gcgctcgcat cctgtaccag
     7681 tccttcggta cagatataat cgctctgatt tcctgcgtta tccatcgcat aagcatagac
     7741 tacatagcta ccatcctcgc ctaatgcgcc atgaatgatt gcactgtccg acagccagtt
     7801 tcctgcatct acctgcgtaa atttaccatc ggctgccaga gtgtccagtt ctgctttggt
     7861 ctttcccgtt gccgaagcag tgtctgtgat ctggtcaata taatagtaat acttagatac
     7921 cccacttacc ttattcacgc cctcttttgc atcgtttgca tgaatcttaa tatccagtgt
     7981 tgtatcattg tagaggtgtc cgaagctgat cttatttaaa agtgttctcc accagttatc
     8041 tttgatctgg atgccataac catcttcctt gccatctgca tctgcccagg tcggtgcggt
     8101 ggtatcaatg tttatcgtaa tctctacagg aacaatagat tgattccatg ggtcttttcc
     8161 atataaagtt acggtatgcg tgccttcttc cttaaaggta taacccggat ttttgcctgc
     8221 aaccgcatct tccaggcttg cataaaacac aacttcatcg cttacaatgg catattttcc
     8281 tttcgcggtc cgatatacta tatttttcca gtcagatact ggattgtctg atgttacatc
     8341 attttccgta aacgtagcag gtatgccgtt tttccaccag ttggaatcct cattgtacca
     8401 acaattcttg gataccgtgg tctgtgccag agtaaacgca tgggcaatgt caaagtatct
     8461 gacgagctca cccttataat tgccaattcc ggtaaaggta atctttccat ctcttttacc
     8521 gtttgtcacc tccgtattat tctcagcact tacggtgaaa tctttgtctt tgtccagaac
     8581 tccctggtca tttcccggct catttttccg gtttgaatct gtgattcctc tatcccagtt
     8641 ttcaggaatg atcggcatac cggtgtaata taccgtgata cctatatcat ccagatggat
     8701 cacggaatct gttaaacttc tcggctcaat ggtcgctttc acgtcaatag ggagagaagc
     8761 acacatattt tttctagctt catgtataga aacataaatt gtatagtttc cggcattggt
     8821 tatcatatga ttctctgtaa ccggatcatt tatttgttta taaccttgag aaactccatt
     8881 ttcataatac ataagtacaa aataataggt aaaattgctg gcaaatccat ccgtttccat
     8941 tgtttttcca gtacagggta cgatcagccc atcagcaacc ggaatcattg tctggctatc
     9001 aacagcgtct gccttaagtc tgacggttcc atactgaaca gctccatggg ctttcccatc
     9061 ataggttccc tgatagccac taaagccttc tcccacaact tcaccatatc caatcgtgaa
     9121 atagtgctca atcgtgccgt aatagctgcc ctgtccggtg atcgtaactt tcggtgcttc
     9181 atcagccttg aaatcgcttc caccttcttc attcctgtag cagtctatat tattggcata
     9241 gaccacataa taatttgctt tggaaatgtc tgcatttgca gggtctgtgg aatcctgtgc
     9301 caattctctg cctgatttat atgagggccc agtcagcgac ttattgccat tcgcagtatc
     9361 cagtgtaact cctgtcatca cctgcggata gctgtcgtag gtacggccta ccagaccttc
     9421 gatatgaagg tgtccatttt tataggcttc cgcaaggtcn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnng cccagccgca gtacagcggt atcaccagga aagcttatgt
     9601 ttccgaccac gtttatattt tctatggtaa catcctgtct ccctgacata aacaggtagt
     9661 ttcccttacc cgtcaaggta tgtggctcat caccctctga acgcagcgtt atctcagccg
     9721 gcaaatatac tttatcgagc atcttgttta tactcttacc tgctatcgta tccttcagca
     9781 gcaggatttc cagcttcttc cataacttgt attcttcatt cttttgatgc tctttcgatt
     9841 cttccagata gaacattgct gcatcccatg tcaggaaata catcgttttt gtctcaccgc
     9901 tatttttttc tatctgaaac tttacaacgg cctgagatgt atcctccggc atgtcagcgc
     9961 gaagcacttt tacactgccc tgacttctat ccgttctgct tatatcggtc ttaataccgg
    10021 ctgaataaaa agcatagcct tcttccagcg tatccgcaat agcataaccc tgcgttttat
    10081 caagcagaga atcatcattt ttaccaaaat tgctcagagc aatctctcca tactcaccac
    10141 ctgaaagcat tacttttgca cgttttatct ggctaaactc tctgtggtct atatgcagag
    10201 cctttatctt tgcagtatct ccggatacgc taagggtggc acctccacat accagaacct
    10261 cttctccctc atacgtacct ccggtaattg tacaggaaat atcagtattg tgcatctcac
    10321 tagcttttcc aatataaata ctgttgctgt aataaccatc ttttagcttt gcagccgcac
    10381 cacccaaaaa cctcaagaca cccttataat taccattctc taccgtaagc tttgctttgt
    10441 tctgaataga aactggggcg atcagtgttc cattctgtcc cgtgcctgcg tctttaaaga
    10501 ctgcctccac accttcgcca gtgatataaa gaccctttcc tgtaacggta cagccattaa
    10561 gatccataca aaagctgacc ttttttgtac ccccatctat tttccactcg ctttctggct
    10621 cagaaatatt tttcaggatt ttcaacgtaa tttcaaggtc tgcatccgca tcagcagtat
    10681 ttactgtagc tttaactgca tcgaatgctt tcgtaagtgt tgcataacgt gttgtattcc
    10741 cacctaccgt aacttccgca acaccttctt ctaccgtaaa gacagccttt ttggaggtct
    10801 ccgtatattc accgcaggtc gctacacagt agtattgata ggtgcctgcc aaaagtcctg
    10861 taggaatcct gtaggtcgca gtttttgcat cctgccccgt ttgttctact ttggttgttg
    10921 ttccatttat tgttttatca gcataccact ggtaggagat actgccactg ctatcactgc
    10981 cagataccgc ttttacggtt agcacaggtg cctcggcagc ggtatagttt tccatcacag
    11041 tttccgctcc gcttgaaatc gtaggatgcg tcgtgaaata gagcggactt ttaacagccg
    11101 ttaccttttt cccttcgtct gtccacctgg ctgttaggtc tgcataggta ccatcctcat
    11161 actttaaggt atagccgacc ttcagaaggt cctgcagggt tttcttagga ttattaaccc
    11221 aaatcgcatt gcctgaggct tgggcagggg taaaggttcc ctctgcaatc tggctgttgt
    11281 cgggtaatct aagacgtcca ttaaaggttc caccctcaat gtttacagct aactttttat
    11341 attcgttatc cattataata cggttgccag cattcgctgt taaagtagcg ccttttttaa
    11401 ctgtaatttt aatgctacta ttttcgtaaa caccgatgca gcgagaatcc ttgttattgt
    11461 tttcaataac tgcgccgccg ccaatgatac agtcggagca gctgcccata aacataatag
    11521 cgccatattt aagcttaacg tttccggtca ccttcgtatt ggtaattgta caggcgttac
    11581 tgtcacaaat agtaatgacg ccttcctgac taccattact tccactaata tttgcttcgt
    11641 caatggtaca ggtcgcacct ccccacatcg caactgtgga accggttcca gcatttttga
    11701 ttgtgacatg ctcaattttc agcacgctat ctacagttat cagtccatct cctttgaatc
    11761 tattttgccc atattgatag ttgcctgtaa ttgttccctc attcgagccg gacgaggtaa
    11821 ttgttatatt tgctctttta aaatataaac gcgcattgtc agccagtttc aatgtatggc
    11881 cattcaaatc cagctctatc tcttttgcca aaccggtacc aatggtaagc gcacttgtta
    11941 tggaggcatc atcattcaac agtttaatgg aaactttttc caggtttgcg tttccatgcc
    12001 cgccaatcca tgttgcaagg tcctgagcgt tgtatttttt ctgtacagtc ccgtcagctt
    12061 tcatttctgt tacttcaatc gttccatcag ccagcagcgc aatcccatat tcgcctacga
    12121 gcacatagat ggtcggaagc tccacgtctt tacccaatac gagctggttg ccgtctccat
    12181 cctcatccgg cagtaccggc gtgtaggcat agcagaagcc gtttgacact tcactgctgt
    12241 caaattcttc tgcgtcgctc tcttccacat tcagctccca cttgatgttt tccagacgga
    12301 ttttctccgt cgttgtctct gtctcagagg aagctgctgc gtcagaactg cctgtcgtct
    12361 ttacactgtc tgcattatcg gatgtagatg ctgtgtcggt tttctttgca ttgtctgcac
    12421 cgtcggatgt ggctgctgtg tcagaggcat cgtccttctc tgccgcgtgt actatgagtt
    12481 tatgcggcag aagggcatcc gccatctggt caataagcgc accaaaagca gagggtaggt
    12541 cggcacgtgc ctgtgtatcc tctgtatcag agctgctaga gccatttggg ttgtttgatt
    12601 tgtcagaatt tccagaatcc tttgatgtat ccgaatcctt tgtatctgca tttccattgt
    12661 cagaagtacc agacttatcg gaagcatccg cattgccgga atcgccatcc ttttcatctg
    12721 aggattctgt atccttctta tcatcagagg acgcagtatc tccattactg tcatccttct
    12781 gcgcatcgtc tttctctgta tctcccgtct cggaagcatc ttcttcatcg tcggcttcct
    12841 tttcatccgc ctgcgtggtc ttttctaccg taacggtcag gctagtcgga aacttgatgt
    12901 cgctctcaag cgcaccgatg ggcagggtct gctctatgat gtcctttgaa agctcctcaa
    12961 aggaaacaat agtcgtttta cctgtctcac tttcatcggt ttttgcagaa accgagagtg
    13021 cggacaggtc tacagtattg gcaaccatcg caacggtcaa gcccatcaca agtatgcact
    13081 tacgcagctc cctccgcctg attttttcat gggtctgctg ccaatccctc tctggcatgg
    13141 tgtttttctt tttcatgtaa actcctatct tttaccataa tctctcggaa tctctacttt
    13201 tcccagcctg attgtgatat cggctgggat tcttcccatg actgttatta tcatggctat
    13261 aatctataca tgcttgttga ttcgcaaagt gacttaccgg tatttcctca tttttgctgt
    13321 gcttcaaaac atgattcaca tggattcctg cacgcttttt ttcaggatga aatcttttct
    13381 tcctgattac actgtttcta aagtactttt ggattctgca catgatgcca tgctagagtt
    13441 ttgctggcat gatcactcaa gattccgaga gatcattata tgtataaggt ttatattcag
    13501 cccgttcaag cacaggcaca gcttttatag atcatccgga atgagccgca gctttttcgc
    13561 ggcggtcttg gcttccgccc ggcgcttcac gtccagcttg tcgagaatgt ggctgacgtg
    13621 ggtcttcacc gtgggcagct tcacgtccat gatctgcgca atctcnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnncaggg cgatggcagt caggacattc aggtgaatgc
    13801 cgtcgatgat gcgggcgcag ttttgaatgt acggctccat gggtgagagc gtcagcaaag
    13861 cggcgtccgg gcggttctgc ataatttcca ccattgcctg cgtgagatac cggtagcgcc
    13921 gcatgacgtt caggtgcgtg ggttcgcacg gggctttttc ccgataccac gcgtccgccg
    13981 cgtcaaggtc gccggtgtgc atgtcaatac ggcagagcat ggcgtccata ttgggcagaa
    14041 agcgtgtcaa accgcgctcg gcaaagcatt cacggagtac catgatggtg cgccgcgcgt
    14101 cggtgggctg cccgtttgcc agctggctgc gggccagcaa tccgctgacg gcgaactcca
    14161 tgtccgacgt gccgtagttg cggatctcgt tcatctgcgg gagaagggac agcatccggc
    14221 cggcgacgtc ctcgcccttt tcaaacttgc tctcggcaat ggcgcagtcg gcaagacaca
    14281 cactgtcccg ccccagtact gcctccacgg ggagacggag ggttttgtag agcaggtcgt
    14341 cctttttgct ccattcggaa aagtccttgc caccgttcat gatgctcggc agcgcgctcg
    14401 tgacggagaa ggacggcagc gccacctctt tgttcgtcag cagccggaac acggcgggga
    14461 tggtttccgt cagacccttc acgccccgct ggggcaggga gatgtccagc catgcaaggc
    14521 ggccgcgcgc ctgctttccg gcggcgtcct gcctgccgca gtgttcgaca aacttttgca
    14581 gttcgccata ccagcgctcc gagccttcgt aatccatgac cagcgcgcag agcatgctca
    14641 tgccctgcat cagagagggg gatgcgagaa tttccgcctc cggcaggctg cggtagtatt
    14701 tttccatctc ggcatagtgc cccatgccgg ggtgaagttc tgcattacgc accaacagct
    14761 cggaaacctt ggaatggtct ccgccgctgg tgtagcattc cagggcgtgg gcgtagtcct
    14821 ccttcagctc atagtacagc ccgccccggc tgatgagcgc tttgcgcttt tcctcggtat
    14881 actcccgttc catctcccag aggagaaaag cccggaaacc cgaccagaag tggaagcgct
    14941 ggcagtcttc gtagcgcagc atagtagtgt atcgaagaat ccagtccagc cgttcgccgg
    15001 cacggggatc gccgctgacc atccgcgcca tttccaggtc aaaactttca aagggggcca
    15061 gctccaacag gaagcgccgc accggcaggt caaagcgccg gtagatggcg gtctcaaagt
    15121 agaggaacac ctcgtggtac acccgtgcca ccagctccgg cgtccacggc ttgtccgggg
    15181 acatgcaccg cgccgtgatg gccacaccca gcggatagcc cacggattct ttcaatattc
    15241 cgtcaatctc gctatctgtt acgtttactc ccgaaagctg aaacagccgc cgcacatcgc
    15301 ccgcgtcaaa aagcaggtcg tcggcctcca gcaccgtcat cagccccgtg tactgaaacg
    15361 ccgtcagaca gccgggcggc acaccgcgcg agagcagcac gaaatggcgc tccggacttg
    15421 agcggatcag ttcgcaaaga gcctgctgcc cggcttcctc ctgcatgaac tgaagatcgt
    15481 caatcagcag aatgtcccaa tcctgcgctg acggtgggat tgcgcagtct gggtctgccg
    15541 cactttgccg cagcacgttc cgaccgcgta gcagtgcgtc cgccagtact gtctttccaa
    15601 agccgcaggg tgcgctgaaa aaaaggactc tgccatgcgc catgaagctg tcaaaccgtt
    15661 tctgaacgga ggtctttatg atgatatttt tggtcatttc ttccctccct tt
//
LOCUS       DAWEJW010000067        15557 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1395_length_15557_cov_54.145981, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000067 DAWEJW010000000
VERSION     DAWEJW010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15557)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15557)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15557
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1395_length_15557_cov_54.145981"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4254 a         3204 c         4360 g         3739 t
ORIGIN      
        1 atcgcactgc gacggaatgg cgtgatgccg caacagtggt aggaaacgga tgcctgccac
       61 agaaaggatc aatatggcaa aggtttatat taacgaaaag aacaaaaagg gacatatcaa
      121 tccggaactt tacggcaatt tctctgagca tctgggaaga tgtatttatg agggactgta
      181 tgtaggagag gattccgaga tccccaatac caaaggcatg cgtaatgatg tggtggcagc
      241 actcaaggag atgaagcttc cggtactgcg ctggcccggt gggtgcttcg cggacgaata
      301 ccactggaaa gacggtatcg gaccgaagga gaaccgcaag aaaatgatca acactcattg
      361 gggtggtgtg accgaggaca acagcttcgg tacccatgag ttcttcgaac tgtgtgaaca
      421 gatcggttgt aagacctaca tcaatggcaa tgtgggaagc ggtaccgtac aggagatgtc
      481 cgagtgggta gagtacatga ctttcgacgg ggtatctcct atggcagacc tgcgtagaaa
      541 aaacggcaga gagaaagcat ggaaggtgga ttacttcggt gtcggtaacg aaaactgggg
      601 atgcggcggc aatatgaccc catcctacta tggcaatctg taccgcagat accagaccta
      661 tgtgagaaat tatgatcaga agcacccaat tttcaaagtc tgttgcggcc ccaatgcggg
      721 agatacttat tggactgaaa atgtgctgaa gacctgtttt gaaaatgcac cggaatggat
      781 gcatggcttt atggacggac tttccctgca ttactataca ctgccggagg atgactggtc
      841 tcacaaggga agtgcactgg acttcgacga tgcagcatgg tataagacgc tggcaaaggc
      901 ttttaagctg gatgaactga ttcataagca ttctacgatc atggacaaat acgatccgga
      961 gaagaagatc ggcctgatct gcgatgagtg gggcacctgg tatgatgtgg agcccggcac
     1021 gaatcccgga tttttatacc agcagagcac catgcgtgat gcactggtgg ccggactgtc
     1081 tttaaatatt ttcaacaagc actgtgaccg tgtgaagatg gcaaacatcg cacagcttat
     1141 caacgtactg caggcagtga tcctgaccga gggaccgaag atgctgcgta ccccgactta
     1201 ccatgtattc catatgtaca aataccatca ggatgcggat ctggtggaaa gctatatcga
     1261 tggtgtggaa cagatcggtg aagatgaaaa gttcaaggtt ccccatttac aggaatccgc
     1321 ttccgtggat aaggacggtg ttgtgaccat caccctgaac aacctgtcca ttgataaggc
     1381 agaagaggtg gagatcgctt tcgcagagtg taatccaaag tacgtgtccg ctgcgattct
     1441 taccaatgat atgcatgctt acaacacctt tgaggatccc gataaggtac acgaagaagt
     1501 atttacggag tataagatcg cagatggcag gatcaccttt accatgccgg catgcagtgt
     1561 cgttatgttc cggataaaat aagcgataag aacagttatg gaagaagaac aaataagaga
     1621 aacaataaaa tattcctgtc ggaaatgtct gttgcgggaa atggacaaga atgcctatat
     1681 ggagaatctg tatgcctaca tagacaggat cgaagaggat agtaaagcag ataaggcggt
     1741 ctatgaagac cgccttgctg tatgtaagga atgcaaatat ctgaatgagg gcctgtgcgg
     1801 tgcctgtggc tgctttgtgg aactgcgggc tgtaatcgcc gtcaaccggt gtccttatga
     1861 gaagtggtaa cttctaagat caaagggtta ccttctcagg aacatatttt ttcagggctg
     1921 cagccacggc ccccacaccg gagcttaatt ctgcaaaata ctgttttacc agagtatcca
     1981 tgccgatggc gtggaggttc acaccgaata ttttctcatt ggcaagtaat gcgtccagct
     2041 ttgacagatc cttgggagag aaactaagtt cgtagtctgc aacgtactga cgggcttcct
     2101 ctaacagggg gtcggagctg ggcgtaaatt ctctgccctc atcatcgatt cccatgagat
     2161 accggagcca tccggcatag accagaggaa tcagcttcag gtcttccgta tgcaggtctg
     2221 gagaagcttc gtaagctttg atggtctcgc cgaagcggat ggaaagcttc tgggaggtat
     2281 cggtagcgat tctctgggga gaatccggca ggaagggatt ggggatcctc acattcacca
     2341 cttcgtcaat gaatttcttc ggatccagga tgccgggatt taccactacg ggaagtcctt
     2401 ctttgtaacc gatgacttcc accatatttt tcaataacgg gttcttcatc tcttcggaga
     2461 tcagggtata tcccagaagg cagccataga tggccagtgc ggtgtgcaga ggattcaggc
     2521 aggtacatac cttcattttc tccactttgt ctacggtcgc tctgtcggtg aagatgatgc
     2581 cgcctttgtc cagtgcgggc ttaccgttcg gaaaagcatc ttcaatgacc aggtattcgc
     2641 attcttccgc attgacaaag ggagcgatat aggtgttttt ggaagtaact acggcatcca
     2701 gaccgccgat atgatcctcg gccagcatag cttctacttt ggcatcaggt ctgggggtga
     2761 tcttgtcgat catgctccag gggaaggtga cttttgtctg gtcattgacg tagcctaaga
     2821 agccggcttc caccagtccg ttgtcggtcc atgccttggc gaacgcattg acagcggcat
     2881 ataatttgtc accgttatgg gagcagttgt ccatgctgac catggcaatg gggagcgctc
     2941 cgtgagtgta tctggtataa agcagggaga ctaccttgcc caggtagctt tcgggggctt
     3001 cgggaccttt ttcgaagtca gcggctactg cggggaagaa ttctcctttg gcattggcgg
     3061 tagcatagcc tttttcagtg atggtaaagc tggccatctg gagagaagga tttttaaaaa
     3121 tctcccggag acgtgcgtat tctgcttcgt ttttactgtc caggatcaga gactccatga
     3181 tgctgcctac gatggtcttt tccacggtgt tgtcggcctt taaggtggcg agaatgctta
     3241 aattgtcgtg gggacggtag cttttttcaa tgatctcgta gtcatagcct tccgctgcga
     3301 tgagaccggt atccagaacg ccgctgttta acagattctg tgctacgttg gcctgaaagg
     3361 cacggaagat attgccggca ccgaagtgga tccagtgggg atgttccacg gtgtttttat
     3421 gaacggtatc gtaatcgaat tccggcagat gataaccggc agcgagaaat gcgtcccggt
     3481 cgtcatgaag actttttaag gtaagttcca taatggtttc ctccaagctg atttgtgagt
     3541 tttcaggtgt gatggttatt tctgcttatg ggacttctcg atagcttccc acagaccgtt
     3601 taaataggtg gcgcctaaag ctctgtcgta cagtccgtat ccgggcatgg ccacttcatc
     3661 ccagatcata cgtccgtgat cgggacggat gggaccgtca aaaccaatat cataaagagc
     3721 cagcatgatc tcgtacatat cgaaggttcc atcgctggac agatgtgcag cctcctcaaa
     3781 atcagtagga gagttgaact tcaggtttct gacatgggcg aaatgaatgc ggcctttcag
     3841 ggagcggatc atatccggca gatcattttc caggttggtg ccgtaggaac cggaacaaaa
     3901 ggtaacgcca ttgtgagggt tatccaccat cttcatcata cgcaggatat tctgcttgtt
     3961 gatgatgata cggggcagtc cgaaaacact ccacgcagga tcgtcagggt ggatcgccat
     4021 gttaatgtca tatttatcac aggtgggcat gatacgctcc agaaaatatt tcaggttggc
     4081 aaaaagcttt tcatcatcca catctttgta catttcgaac agttctttga catgagccat
     4141 acgctcaggc tcccagccgg gcattacagt gccgttcata tcgccggcaa tggattcgaa
     4201 cattttggcc ggatcaatag cgtctacagc agcctgattg taagcaagga ctgtagaacc
     4261 gtcgggacgc attctggcaa gctcggtgcg tgtccagtca aagaccggca tgaaattgta
     4321 gcataccata tggatgcctg cctggccgag ccgttccagg gtagtgatat aattctctat
     4381 atattgttca cggtcgggag aaccgatctt gatggcatcg tgaatgttga cactctcgat
     4441 accggccagt tgtaagccgg cagcttcaat ctcagtcttt agggccagga tgtcatccat
     4501 ttcccagact tctccgggag cggtgccata gagagtggag atgactccgg tgacaccggg
     4561 aatctgacgg atctgtttta aggtaaccgt gtcgtacttg ctgccatacc agcgtaatgt
     4621 catttgcata atagaaatcc tccttgtagg gaatgctttg gtttcgtttt ttgctgtctt
     4681 aagcatagca gaatggcaaa tgcattaaaa ctgcatgaat tgcttgaaat agtgcaaaaa
     4741 ttgctatata gtaagacagg ggatataaac catggaaaat aaaaactgaa cagaatgata
     4801 caggagagaa aatggatggt cgtgaacaga aaaagcagcc tgcgctgcag ggatgcggcg
     4861 gacgaacggc aatatatgtt gagcggtgac tatgaggtct atgagaaggt aggggagcct
     4921 gtcggagcgg tgtcgttcca ttatcatgat ttttatgagt tgatctatat tatagagggg
     4981 gaattttctt ccctgatcga ggaccgggcg gtgcggatgc ggaagggaga tttcctgttg
     5041 atcgataaaa atgtcatgca caaatatcat cccacggaga agaaaatgga ttcgtcccgc
     5101 cggatcattt tgtggatcag tgatcagatg ctgcgggagc tggcggcggg agagccgttt
     5161 cttgcagaat gcttctgggg agcggggccg aaactgtatc atttccccat ttattatgag
     5221 gaaattttgc gtggatttct gctaaagctt gctatgtcgg agatcttaga gggggatgat
     5281 gtccagaact gcggaatccg taaggtcatg gacagaggca atttgagcct gttttttggg
     5341 tatctgaatg tgttgtgtaa acgggaggaa tatttgtctt ccacacagga gatcgtggat
     5401 catcctctgg tggagcaggt gaccggctac atcgagaccc atctcagcca ggagatcaca
     5461 gtggaggaag tggcggaggc cgtgcacatg agtaagtatc attttctgcg gaaattcaag
     5521 gagatcaccg gcatgacggt gcataacttt attgtgaaca agcggatcat ttccgcctgc
     5581 caggccatgg cggaggggaa gaacattact gagagctggc agcagaccgg ctttgcggat
     5641 tactcctctt tcctgcggaa tttccggaaa atctatggca tttccccaaa agagtaccgg
     5701 gaaaataacc gcagcttgat tatttcccag taaagaggtc gattgccggg gcaccggcag
     5761 catgaaacag aatgtggaaa aataggattt taattatgct aagtaagctc atttaatgca
     5821 aaaaatatca taatatgtta tactgtattt gttatgcgct gagcactgaa tgaagagaac
     5881 aaatgataga agaacatagg gatgttatga tggaaaatta tcaggaaaag tataaaaaag
     5941 aattacacca gcaggagatc aactcaaata tacacacact aaaaggtttt ttctggattt
     6001 ttgtgactat tctgctcctc tggatgttga cattagtgag aatctttatt gtggattcca
     6061 gaatctttac catggcagca ggggtgtcgg cggtgatggg aattcctgtt ttatacattt
     6121 ataaaaaggt ggatctgtca aagcattggg taaagtatgt gtttttgaca ctgatctgta
     6181 tgatatcggc agtcattgcg gcatttttgt cattccatgc ggtgcttatc tatgtgctgc
     6241 cgttgctttt ggcggtacag taccgggaaa ggatgacact gtgggttact tatgtcgtga
     6301 atgatgtgac tatgacactg agtatgctgg cagggtttta tcatggaatc tgtgatctga
     6361 atcttctgct tggcagtaat cataccagag actggtatat ggagcagtgg ggggcaggaa
     6421 cactgcagtt tagtctggaa cctgatcctg tatttactat tttgttctac ggagctttgc
     6481 cacgggcagt gatccttctg gcatttacct ttgttttgcg gtatatcagc attaccagcc
     6541 acgaggatgc acagaggatc gccgacctga cgtatcgtaa ggagactgat ctgggaaccc
     6601 atgtgtataa taagaacaaa tatgaggaaa tgattgctga ctattatctg ggggttgaca
     6661 gactggctgc cattttctgg gatgtgaata atctgaaata tgttaacgac caatatggac
     6721 atgctgcagg ggatgtgctg atccagacgt tgtcttccgt attatatgaa ttatccacgg
     6781 acagacgtaa ggtctatcgc ataggcggag atgaatttgt catgctgatc gagaatccgg
     6841 tagaaacaga gatcgagtca atggtagagt ctgtcagtgc tgcattgcag gagaaaaaca
     6901 gtcagggcga gatgccggtc tccagtgctg tgggctgggc agaaggctgt ggtgtggatg
     6961 tccgtaagat catcaatgaa gcggatgcaa agatgtatga gaataagacg cgtggaaagg
     7021 aatgcagaaa ataggatggc aaactgggta agtgcaggct gcggtgtcat tgcaaatgag
     7081 ctggcgcagg ctatggaaaa aagaggacag aaactgtatg gtgtcgtgaa ccggacgccg
     7141 gagaaggcag tggcctttgc ggaaaaatac ggtgtgcaga aggtgtttac gagctttcag
     7201 gatgtatgtg cggatcctgc ggtggatatc atttatattt ccacacccca taacactcac
     7261 attcactttc tgcggaaagc gctggcagcc ggcaagcatg tgctgtgcga aaaatccatt
     7321 acgctgaatt ccgaagaact ggaagaggca gtgcagctgg cgaaggagca tggtgtggtg
     7381 ctggcggaag ctatgactat ctatcatatg ccgatttata aagaactgaa aaaacgcatg
     7441 gatgccggaa aattcggaga attgcgtgtg atacagatga atttcggaag ttataaggaa
     7501 tacgatatga agaaccggtt cttcaaccgg aatctggccg gaggagcatt gctggacatc
     7561 ggtgtgtatg ccctttcctt tgtcagaatg ttcttaaatt cctgcccgga tcagattact
     7621 tcgcaggtga agcttgcacc taccggtgtg gacgagcagg cgggaatact tctgatgaac
     7681 cgggagcagg agatggcaac ggtcacattg tccctgcatg ccaagcagcc caaacgtggc
     7741 atgatctctt ttgacaaggc ttacgtggag atgtatgagt atcccagagg gcagaaagca
     7801 gtgatcacgt acacggaaga cggacacacc gaggaaattg tggcgggagc tacggcagac
     7861 gcactgggat acgaggtgga ggatatggaa caggccatcg ccggacatcc ggaattaatg
     7921 aagcttgagc ttaccgagga tgttatgaaa atgatgaccc ggatccgaaa agactgggga
     7981 ctgacttatc cagaggaaga gcatgccacg gaaaaaatct gaggagcatc cctgatgcag
     8041 atcctgacag gctccatgat ctctgtggtg atctgtatgg cagcatttgt atggatgaaa
     8101 aaacacccgg gaaatgctga cttccgggtg ctttatctta tctcgctaaa cttagaagaa
     8161 ttttttgcag cgcataagag tcaccatcca taactgtaaa gcgggctttt tttggtggct
     8221 catctgcgga accggaagag gtggcggtgt gcaggtaaca gcagtcgatc cccgcagcta
     8281 tagcacctgc caggtctctg gtacgctcgt tgccggtcat cagacaggtg gtgaaatcta
     8341 atccgtagcg gtcaaacagg atccggaaaa aggcgggatc cggcttgcag cagccctcgt
     8401 cagaggagat aaggataccg tcgaacagat cccagagtcc cagcgagcgc agctcgtatt
     8461 cggtgaagat ccgctgggca ttggacagga ggtagatccc acagcctttt gcatggagct
     8521 gctgcagtgt gccgatcgtg gcgggatagc attgtaaaat attgagggat tcgatccgga
     8581 agaactgacc ggtagcggtc agaagagcct ctgtgggttg gatccctttt tcgacataaa
     8641 gagttcgaaa aatatctgcg gcgcagatct ccggacaggt gtaagatgag ggaaccttgc
     8701 tggcagccgt ttccagctgg cgatatctgt tcctcagttc cctccccgta taggaagcac
     8761 cctggaagga atagaacagc gcaagccttt tccagaggga agttttattt tcattagtgt
     8821 ggatgtctac taaggtgccg tagagatcaa agataaaatt atgatacatg gaagacctaa
     8881 tttcttgtgg tgaaaaacct tattccaggg aaaatcagga cggctctttt ctcaagcgga
     8941 ggcagagccg atagatatag tatagcattt cttgcatttt tcaacaatta aaaatataat
     9001 gataccaggg gcaaaaggtc taagcccaca cgagcttgct cataagtggg tgaaagacct
     9061 tttgactccg acggaactat tcagaaccat gaaattatga agcttttgaa ccgctggata
     9121 aagaaaacag gagcgaccgg cggtatgcag ggagagtacc gatgatacga aatactggtc
     9181 cggggaagag acttctggca gcagggattg tacttatact aggcatgacc ggctgtggaa
     9241 cagacaatga attatctctg acagggacgg aagagacagg aatagaagta atggccacgg
     9301 tggatttcac agaagaggct gccaccgggg agaatgcacc ggatgtcacg gatggggatg
     9361 ccgccaccgg ggatgcagag cagactacgg cggaccgcat ggcgggacgg acgaaggtaa
     9421 agggaatcta cgtcaccggc ccgaaggctg gcagtggcgg aatggaagat ctgatccgcc
     9481 tggtggatga gacggaactg aatgccatgg tcatcgacgt gaaaaacgac gagggaaatt
     9541 tgacgttccg gctgactaat gaggagactc cacaggacat tcccgtgctg gaccggatat
     9601 ccgaaatgca ggccggaata cgttacatcc gggacattca gacaatgatg caggaattga
     9661 aagatcataa tatatatacc attgcgcgga tcgtatgctt taaggatccc attctggcgg
     9721 cggcacagcc ggaactggca ctgacgaagc cggacggtaa gccggtgaca gatgccaatg
     9781 gactggcctg ggtaaatccc tacaggcagg aggtctggga atatctgaca gagcttgcgg
     9841 agatggcggc agatctgggt tttgatgaga tccagtatga ttatgtgcga ttcccggtag
     9901 gttcggatgc cgatgcggcg gattacggtg tggatatgga agcttatccg aagcggcagg
     9961 ccatacagga tttcctttct tatgcgggag acagacttca tgaaaagggc tgtgtggtca
    10021 cggcagatgt cttcggcact attatcggca gcgagacgga tgtgcagacc gtagggcagg
    10081 attacacggc actgggacag acagtggatg ccatcagccc tatggtatat ccctctcatt
    10141 atgctaacgg tgtgttcggt ctgaaggtgc cggatgccca tccttacgaa acggtatcgg
    10201 ccgccatgca gggttctgcg gaagaactgc agtcgatccc ggaagcggaa cgggccgtgg
    10261 taagaccctg gctgcaggca tttacggcca cctgggttcc ggggcatatc tcctataacg
    10321 gaacccagat ccgggaacag attcaggcgg tctatgatgc aggatatgag gaatggatcc
    10381 tgtggaatgc aacgaaccgc tattctccgg atggattact tgccgcagga gaagagaagc
    10441 aatagcggca gaagctggct ttataacaaa tccgggcagg agcacaggca ggagcctggt
    10501 ctgataggag cataagatga aagtagtcaa taagatcatg ataggaattg cagtgttgtg
    10561 tctgctgttg tgtggcggaa tcctggtatt cgcattgaac cccgatatga ccagttctct
    10621 ggcgcagaaa ctgtatggca atacagtgga tatgccccct gggaccgaag agaccgggga
    10681 aggacagact tccgggaatg gaaatatcag tgtgacgctt cctaatggta tgccggggga
    10741 aatgaacggc tacgtggcgc cgtccatgga tcagatgcag attccggagg atgtcagcgg
    10801 caggacagga tttactcccg tgcagccgga agaacagcag attccggatc aggaagcaca
    10861 gaatctgaag gaaacattag gttccggaga aacgggagag gggctgtcat tttccgcaga
    10921 ggagtatccg tattatcaga tgttgtcgga gaatcagcag tccgtatacc gacagattta
    10981 tgccaacgca cagaatctga cggaaaagtt tgcaccggag aagaccgtgt ctgccagtga
    11041 tgtaaagaca gcgtttgagg cgatgatcgg tgatcatccg gaaatgttct ggctggagac
    11101 gggttattcc agcaaatatc tggccaacgg gcaatgcgtg gagatcgatt taaaatataa
    11161 cagtacggca gatgatctgg agagcgcaaa gcaaagattc gatgcggcgg cacagaatct
    11221 gattaccggg gcagcgtccc ttgacagcaa ttatgaaaaa gagaaatatg tgcatgatgc
    11281 actggcatcg gcagtgacct atgatctgac agcggatatg aaccagagtg cttacagtgc
    11341 actggtgaat gggaagagcg tgtgtgccgg ttatgccaga gcttatcagt atctgttaca
    11401 gcagctggga attccctgtt attactgcac cggttattcc ggtggggatc atgcctggaa
    11461 tatcgtgaag ctggaggatg gttattacaa tgtggatgtg acctgggatg acgcagcggc
    11521 catccgatat gactatttta acaagacgga tgcagatttt gcctccaccc atgtgagaca
    11581 gaatctgtcg gtgtatctgc cggcctgcaa cggtaccgct tacagacagg aaaataccac
    11641 aggggcagcg ggtaccggtc agccgtctga agcaggtgga gccacaccgg atccggacgc
    11701 aggcaccacg ccctccggcg gtcagacaga cgggagcgtt acagatcccg gacagcaggg
    11761 agacggtacg caggaaccgg agcagcctgg cagctctctc agtgattata tcaatccgga
    11821 tcctcaggag ccgttgcgtt atccctccgg caataccgca ggctctgcag gcaataccac
    11881 aactgccgct acgccggagc ccagagccga tgcgctgacg gatttaagtt cctattatga
    11941 agactgcagg aagcagctga ccggcctggg aagtggtgac cagcattttg acaatgttgt
    12001 gcctaagagc ctgtggagta ccattgagca atcctatcat accggagcct acgagcaggg
    12061 atatgtggtg gatgtgttga aaaatctcgg tatggagtat tttgcaatac agctgcagct
    12121 ggtggatatc ggagacggat attacagagt gtatcataac gttgtgacct attaggaaac
    12181 agataagaaa cactggtatc gaaaggtatc ggtgttttta aattatagaa ataaaaataa
    12241 gaatcattac taatattatt gacagataat ttcaactgtg ttattatcag gataaagaaa
    12301 ataagtaatg ttaagaaaat aaaatatggt agataagaga tactaaaaag gaagcgaaac
    12361 aggctgaagg agaaaaggag agaatataat gaataacaag gcaatgcaca agttaagtta
    12421 tggattatat atcgtaacga cgaaagatgg agagaaagca aacggttgta tcgtcaatac
    12481 tgcgattcag gcggcatcca ctccgaatca gctctgcata tgcatcaata aggcaaatta
    12541 tacccatgat atggtgttaa agaccggcgt gttcaacgta tcggtgctta gtaagacagc
    12601 actattccct ctatatcagc gctttggttt ccaatccggc cgggaagtgg acaaattcgc
    12661 agactacaca gcgtataaaa atgcggagaa tggcgtgccc tacatcatag aaggcacaaa
    12721 tgcttatctg gcggtaaagg tcagtcagac cgtggatctg ggatcccaca ccatgttcat
    12781 tggtgaagtg acagagatgg aagtgttaag cgaggagcca tcggcaaact atgagtacta
    12841 tcaagaagag gtaaagccaa agccggagga agtgggaaaa gccgcagacg gccagacggt
    12901 atggcgctgc accatctgcg gttacgaata cgtgggcgag gaactgccgg aagattttat
    12961 ctgtccgctg tgcaaacacc cagcttccga ttttgaaaag gtgatcaaaa attaatccag
    13021 tggttttaat aaggtgaaat gactgcgctg tgtccggagg atgccaagct ttgtgagctt
    13081 gcccagctct gcggacatgg cgcttctttc taccccgagg taatccgcca gctcctgccg
    13141 gttgaaaggg atggtaaaat cgttgctcct tttcaatttt gcctgatcgg taagataggc
    13201 catcagtttt tcccgggtgg tcttacggga ggtatatttg atcttctgat tcagatacat
    13261 atttttctgc gctacgattc ctaggagatt gcgtatcagg gtatgatgga aagtgcagct
    13321 gttgtcgcag gtgtttaaca gtgttttccc atttaggaac atcactgtac agtcctctgc
    13381 tgccacaata tccacgggaa gatatggaat tcctgcacag gcaaaagcct ccccaaacag
    13441 ggaatccggg gggatagaag cggtgatatt gcggttgccg taataatcgt cctgtaggat
    13501 gtgaacggag ccggattcga taatgccgat gaaatccgca ggatcaccgg catgcttgat
    13561 gtattcttcc ttttgatagg atttcgtata tgcattcaga cgcttcagca taacagggat
    13621 atcttcgggt aaaatatttt gaaaaagagt gctagtcata atgaattctc cttttgttgg
    13681 aaaatcaaca tacttgatgg gatgattcta gtataatcac ctcagaaagt caaggagagc
    13741 tctggtggga gcatgccggg caggcacaat aacgatgtgt ttaagaaagg atggacaacg
    13801 atgtataaaa atatcgacaa gcagaccaaa ttaactctga aggagctggt ggaataccag
    13861 gagggccagg tagtcagcaa gactctggta cagaacgatt atgtgagcat gacaatcttt
    13921 tcctttgaca agggcgagga gatatctacc catgcagcag gcggagatgc catggtgacc
    13981 gttctggaag gcaagggcag atttaccgta ggcgacgagg tattctatgt ggaagagggc
    14041 gagacactga tcatgcccaa ggatattcct cacgcagtat acggtgaaga gagattcaag
    14101 atgcagctga ccgtgtcttt ctaaattata agtcgtgctg tatataccgc ttgtgtggaa
    14161 ctaatattca tgcaggcggt atttttttgc cctttgtgtt cagaatttta ccgctggagc
    14221 tgggcgagaa catgatggtg cccataggtt ggactggttc actatcggtt ctttttatga
    14281 ggccattggt cataagctgg aaggagaagc gctgcttacg attaatcgca ggacgctgga
    14341 gaagatgatt ttgtatgcga agaaaataat gattttgttg gaaaaacaac ataacatccg
    14401 gcttaagtct gttatggtat tcgaagagac aaagagaaca gcaaaattaa ggaggcaaca
    14461 tgattcgaaa aatcattcat attgatgaag aaaaatgcaa tggttgtggt atttgtgcga
    14521 ccgcatgtca cgagggtgcg atagatatta ttgacggcaa ggcaaagctg gtgagagagc
    14581 atttctgcga tggcttcggt gactgcctgc ctggatgtcc tacgggagcc atcacttttg
    14641 aagagcggga ggcgccggcc tatgatgagg cggcagtaaa acaggcacag cagctgaaac
    14701 aacaggaaaa gatgcaacgg gaaggtatgc agattcccca tggaggatgc ccaggaagca
    14761 gaatgcgtca gatgaaccgg acggagaacg cagaaattgt ggcagagagt agtgcgacaa
    14821 tgaaatccca gttgtgtaac tggccggtcc agatcaaact ggcacctttg caggcaccgt
    14881 attttgacgg ggcaaaactg ctcatcgcgg cagactgtac cgcatatgct tatgcaaatt
    14941 ttcataagga gttcattcgc ggcaaagtga cgctgatcgg ctgtccgaag ctggatgcag
    15001 tgaattacag cgagaagctg acggagattc tgcggaacaa cgacattcag tccgtgacca
    15061 tcgtgcgcat ggaagtgccc tgttgcggag gactggaaat ggcggcaaag aaggctatac
    15121 aggacagcgg aaagttcctg ccctggcagg tagtgaccat ctccattgac ggagagattt
    15181 tggaataaac aggattgtct gtacggaaag caggcatctg aaaaatggag gtttgtagga
    15241 aaatgcaggg agacagaatc atcagcgccc tgatcggctt ggtgggagcc gtgtctaata
    15301 atggcaaaac agagcggacg gacagtgtgg tcagggaagc atttctgcgg ttgacagatg
    15361 gcgatagcga agaagagaca gtccagaaga ttcatgcgga gaaattcgcc attgcaccgg
    15421 actgcgctaa ctgtctcaac ccctgtggca atacttcaga ttacgatatg gcgcaatttt
    15481 acgcagcaga tgcgaagatc atcgcggcaa agcgggatct gattgaggct atttgtaaaa
    15541 aaatgagttc atcggag
//
LOCUS       DAWEJW010000068        15357 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1412_length_15357_cov_50.416285, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000068 DAWEJW010000000
VERSION     DAWEJW010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15357)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15357)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15357
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1412_length_15357_cov_50.416285"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1803..1902
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3759..3858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4012 a         3829 c         2911 g         4405 t
ORIGIN      
        1 gtgtcatgtc cgcttccgac tccaccgacc tgcataagcg cctccgttcc tgttttccgg
       61 ttgatctgtt ctactatgta tatcggaagt attcactttt ttattcagtc tcccaatgaa
      121 cagatggcct ttttcttttc ttctgtggct gtcccaacag cgtcacaata acatccgtgc
      181 catcatactc ttgtatatct tctcaaaatt ttgatatcac ctggtatcaa accggacaat
      241 acaaaaatag cggaaagcct tgatttatca agtttcccgc taaaaaaata gagcgggtga
      301 tgggaatcga acccacgtat ccagcttgga aggctggtgt tctaccattg aactacaccc
      361 gcatgtttat atatgaagca gggcggccat tttaagaaat ggtgtcctgc gaaaatgccc
      421 agaaccggaa tcgaaccagt gacacgagga ttttcagtcc tctgctctac caactgagct
      481 atctgggccc gcgacttaat cctacttgca atcttgtttc atgactgctc tgcgtcacaa
      541 caatcgtagt ctacacaaat ttcgtccaaa tgtcaagtac cttatcaaaa aaattttaaa
      601 tttttgcaat tattcctcta tctcctacaa atgatacggt taaaactcct ctttttcccc
      661 gtaccattgc agtaaaagct ccaccacccg gttgtagctg atcatgccat ccgaaacacc
      721 attcaatttc agtgaagcat tggtcagggt atctgaaaca ctgtccaccg tctccgtatt
      781 caccacagct tttccgttga ttctctccca ttgctcttct gtgacaaaga tattgtcctc
      841 ctgcacctgc tgtaatacct gtaacggatg taccgcctct ctggccgccg catagctgtc
      901 cggatccgcc agtctcgtct tatacagatc gtttgccaca taattcagca cacttaagta
      961 acccgcatat tgaaaagtac tgtcatccga ctctacgcag gccagaaatg caataaaatt
     1021 cgcctcatcc tcatagatat acccccggat gtgagccagc tcatgacaca tggttgccgg
     1081 tttttccatg atgtacatga catcattgta atttgcctcc atggaaaacg ggaaataata
     1141 gccacacata tacatctgac acatgaaatc cgagaaaaac atagccttcg gccggggata
     1201 ataaccatcc agctggtcat accttttccc caggctctgc atcacatcga tggctttatc
     1261 ctccatatcc accgcattac cccggctgtc cacaccaccc gagtacacca cggcaccact
     1321 gtcatcccgt tccatgacct cactgagctc attacagtgc cggacgatat cattatagat
     1381 ccgaagcagt tcctccgttc gttcctgtgt tgtctgctcc tgcgcttcct cttcaccgaa
     1441 atatttctcc gaaaatgtag acccatgata gatcatggta caatttagcg tcataatggc
     1501 aaatacggca agcaacgtcc aagcaaaaaa aacaaaatat tccttcactc cccggcagta
     1561 cttttccgaa tgtctgcacg ccggaatcag caaactgcat cccagcacaa ccgcagcaag
     1621 caccaccaga atccctgcca caatcaacca ttcgcccacg gaaaagggaa acaaccctgt
     1681 aatccttccg taggtattga cccagattgg gaaaatataa tcaatataag catcgctgag
     1741 gcttctgctg ctccaggcaa cgacctgaag cagtaaaaga actgcaacga tgcttaaata
     1801 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatccttgg aaaagattcc
     1921 gacaattgcc gtggaaatca tatctcttga taagatcata ccacctatga acaaaaatac
     1981 aaccgatatc cacatggaaa accgatatcc cttctccgca cggtcctgct gctttgctcc
     2041 catattctgc cccacgatag tggccgttgc cgaaccaatg gcattggagg gtagcgtgat
     2101 cagtccattc accttgttgc caataccgta agccgccatg gcttccgtcc cgtagacaaa
     2161 cacgttttta ctcatcagca gaaatcccag ctgcatggta cttccgccga tagcggtagg
     2221 cagaccgatc ttaaggatca ttcccagttt ttccctctgt aatcgcatgt acgcacggtt
     2281 caggcagata tcattttcct gtctgcacag aagtccgaaa gccaccatgg ccggaatcgc
     2341 tttcgccaat agcgtagcca gtgctgcacc ggcagttccc agatccagga tgatcatgaa
     2401 aaacggatcc aggaacagat tcagcgtaat acctcccaga ttaaggaaca tggggcgcac
     2461 cgtatctccc tgtgcctgat gtactgcgga aaaaatgttg accatatata aaaagggcat
     2521 atcccagatg accacccgca gataaactct tgcataatat gcagttcctc cgtcagctcc
     2581 cagccagttg atgatagcgg gtgttcccag aaaacacagt gtggcacaga ccacggcaaa
     2641 tcccatggca caggcaaata tctgatttgc catggatctg gcatcctctt tttccccggc
     2701 accgatatac tgcgcaatga ggactgcacc ggccacagtg atgccgctgc cgaaattcgt
     2761 aataatattc tgcatcggtg tcacaagatt gattgccgcc agctgctctg ttccaatttt
     2821 tcccagccag taagtatccg tcaggttata catcgtctgc aggaagctgt taatcactac
     2881 gggaattgcc agagacagga tcgctctgat cagatttccg tgcaatatca gatccggatt
     2941 atatttttta gccgttttca tcgccatcac tcctaagttg ttcttatcta tgtctttcca
     3001 aatctaagtc gtattatacg tcaatttttc agtggtttcc acggagattt tagggttata
     3061 tgtattaaaa cactttttta ttatcttttt tatttacatc atggaaaggc gcatcgccag
     3121 acggcaatgc agattcttgt gcaggcacaa atcagtactt ggctcatcgt atacgcaaaa
     3181 aaaccctccc acagcatctg cctgcggaag ggatcttttt cttaattctg aagtcatggt
     3241 cagacttatt tgattacaag gctgctccaa ccatcgctgg ggatcagaga gatataggtc
     3301 ttaccggtat tgatctggat ctcctcaccg gtcttcgcat catagtaatg tgtgatagca
     3361 ttctcggaag acttggtcca ggtaacttcg atagccttac cctgagtaat gtagtaaccg
     3421 gagcgtccgg agtcgatggc attgtagatc aggtagcctt cacccagttc tgcaaaagtg
     3481 gtatcctgca gaataacgtt ctcgaaagtg agctgtgcat tgctgtcagc atccttgtgt
     3541 gcagaaccat actcatagta atcataggtt ccggtctctt cgttgtactt cagagtagaa
     3601 ccgttgtgct tgaaaggaag ctcaatggta gtagcgctga tagcatcact tctatcatca
     3661 aaggtaactt ctccgtctgc aaatttgaag tgaggtccct gatagtaatc attgtaagta
     3721 gtggtatact tggaatttgc aaaacgctgc ttcagaccnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnag taatcattgt aagtagtggt atacttggaa tttgcaaaac
     3901 gctgcttcag accgtcatag gtcttgccct tctggctgtt ggtataggta tcataagtaa
     3961 tatactcggt aaactcagtt gccttaccat tgttgaaacg ggcgaaacca ccactgaagt
     4021 tagcggaata atcttttgct acccagtcat tgatgaagta agggccacca tcatggcaga
     4081 gaactgcgtt ccactcagct gccagcatga agttggtagg acgtgcacta cgaatactac
     4141 ccagctgctc gatcttctcc cagtccttta cgactaccat gaaacgtgta attctgtcgt
     4201 tcagagtact gttcatgatc tcatatacaa catctgcatc tgcggtgccg aaatgaggca
     4261 gtgcggttct ctcgttatct accattaccg cgataggacg ctgatccttc agatcctcat
     4321 cgatccactc gttggtaagt tcgctgcgat aggagtgctc cggtgcaact tcatccgcgg
     4381 aagactcagt tgtggcaggc tgctctacct ccttggtatc ggtagcaata ggctctgctt
     4441 ctacggtctg gatcggagaa gagatcggct cactgtttcc acagccggtc agaacgccta
     4501 ctgcaattaa aattgctgca agtactttct ttttcatgaa tgcttccttt catctgtttg
     4561 ttctatccgg tctctcgacc ggcactctaa aatacattgt atccctaaag tcctatgttt
     4621 gtccaccgaa aattcttaag attttcttga gattttattt cgcaaaaaat gacatctggc
     4681 agttgtatgc tgatgtcatt tttgctttct ataattatta cttattattc ttttacagcc
     4741 ccgatattta ctccctgtac aaaatatttc tggcagaatg gataaatgat cataaatggt
     4801 agaattgcta caataaccgc tgcatttttc accgtatttt ccgttgctcc ttctaccgct
     4861 gatggaagat cgcttcccat gatcaatgcc cgcagtttca tctggaagtt atataaattt
     4921 gaattcgtga tatacagctt atagttcgtg taatcattcc agtatcccac ggcatacatc
     4981 agtccgatgg aagccagtgc agccttcgac aacggcaata cgattttata caaaatttta
     5041 aaatgagagc aaccatcaat ctctgcggat tccatcaggc tgaccggaat tccctcaaag
     5101 aaattcttca tcagcaccag attatataca ttgattgccg gcagcaggat cactccccac
     5161 aagttgtttg tcagatggaa gtccttcatt accagataag tcgggatcat acctccgtta
     5221 aatatcatgg taaacaacag cacattggca ataaatgccc gccccggcat cttccactga
     5281 atcagcacat acgccccaag tactgagatc agcaatccca tgatcgttcc cataaccgtt
     5341 gtgtaacagg aaatcaggaa tggtctgagc atcgtcggat tggtaaaaat tgatttatac
     5401 cctgcgaacc cgaactgctc aggaaacaac ttcatgccat aagccgtctt cagatcaaaa
     5461 gagtccacca gtactttata taccgggatg atcatcacca gcgaaaatac tgtaaatatt
     5521 acgatcaata cgatatcaaa aattttcact ttctttttca tcatacaata cctcttcctc
     5581 tcgtcttatg gcttaccctg ttacatacca gtaccagaat acagcttata atagaggaaa
     5641 acataccgac cgctgtcgta tacccataat ccggaattgc accggagtta aatgtctgcg
     5701 tatagatata agtctgcaat acatccgata ccttgataac ggcatcattc tgtaatacga
     5761 aaactgactc aaacaaattc ataactttcg ccagattcaa tacaagtacc gtcacgattg
     5821 tattattcaa cgcaggcagg gtaatataga tcatcttctg gaaacggttt gccccatcca
     5881 tcgacgctgc ttcatacaat tccggactga ttcccgccag cgttgccatg aaaataatcg
     5941 ttccccagcc tgtatctttc cagacattaa ttatgtaata cgaaaatctc caccatttct
     6001 ggtctccgag gaaatatata ttatccgtga tcaagtgtaa tttcatcagg acatcattga
     6061 ccaatcccgc agatgtcgga gacaggatca tggaaaatat ggatgctgcc actacccagg
     6121 acataaaatg cggaatgtat acaattgtct gaaatacctt tttcaacttt aaaaaaatca
     6181 attcattcaa taataccgaa ataataactg ccgcaccggt attcaaaatc agctttgtaa
     6241 tactcagcac cagcgtattg ctgaacgcct tccaaaaccc cggcatactg aacattttta
     6301 caaaatgctt cattcctaca aagatcggat cattgattcc tttatatgta aaaaaggcat
     6361 aacggatacc aaacatagga agataataaa atatcaatgc tgccaccaat accggtgtcg
     6421 ccatcagata gactccccga tatgcacggg cgtgcttgaa cgcggttccc ccaggtgtcc
     6481 tcttaggcaa agtttttcct ttctctttca caacgtttac cctccgttcc ggctactcta
     6541 tatcagctat tcaaaaatcc catatattcg ttgtactttg tctcttctgt tttattcata
     6601 tgtatggtca gtccatcctc tgagaaatag agacggatgc ggatggatgc aacactttct
     6661 ccgatcagcc agcagaagat aaacagcgta ttttgatcca tccaggctcc ggatgaagca
     6721 catttctcct tgtatatagg gaactctccc tcttccagat gtccgattcc aaagggaagc
     6781 tgacattctc ttccttctat ttcatacgag agtactcctt tttgatccgc atcggaaaaa
     6841 gtcacaccac accatgtaaa tccatactta tttcggagca gcggatattt tttcccgaaa
     6901 tctgcacaag gttttccttc aaataccgta cgcagcacag gcaacgttgt tttctccaca
     6961 tcgttttccg ggaaacaatc cttcagcagc acttctctca ctgcggcaag tatcatctgc
     7021 tcggctccgc ccataccctg tgtatctgct gtcgttacaa cgacgagatc catttccgga
     7081 actgccagca ttatctgacc gcccagcccc agcattgcaa aagtcccctc catcgggatc
     7141 cagaactgat atccatacca gcgatcatcg ccatcaagct gtgttgccgt tctcggttct
     7201 gtcgctctcg ctgcatagga tcccttttcc tgctttaaca gcatacagcc ggtacgcagc
     7261 agatcctcgg gagtagccat caggccactc cctcccatag aggttccaaa gggatcctct
     7321 atgatataag actcctcgct gaagccattc tggcgcagca acttttcttt caggtaatcc
     7381 agcatctttt caccggtcag tttttctacc agcgcacaga gcacatggga ggatgaggta
     7441 tcatacataa agagagttcc gggtctgtgg gtgggttctg tctggaaaaa gctcctcacc
     7501 cagttttccg ttgtgctggt cttgttataa gttgtcatat tgtaacatgt ctgcattttc
     7561 aacatatttt ctatcgtcat ctctgccagc caaggatgca ctctcccggg cagatattcc
     7621 gggaaataat cacagatagg atcctgcagg gaaattttcc cttcctgctc cagcaatccg
     7681 attgccagtg aagtaaaact tttagtcaca gaaaacattc tctgcaactt attacggcaa
     7741 tagggtgcat aatatttttc aaacaatagg ttcccatgtt gtgcgatcag cacactgtgt
     7801 aaaggaattt ttcttttttc cacttctttt acaaattcct gcatattctg ttccgtcagt
     7861 tctctcattt tagctgccat accataacct cccatgctgt cgccttttta tttattattc
     7921 agggaatcta cgattgcctg agaccattcg gctccgcctt cctgttcaaa ccgcttgtat
     7981 ccctcttcca cagaaacacc ctgtaccacg cagtccgcaa taatagagtt cttcaggctg
     8041 gtcagatcgc cgttatactg gctcatcata tctgttgtta ccggcaaagg tgccgtcttg
     8101 ttattggcat taaagatttc catcgattcc tctgtatatt tctttacggg aaccagtctc
     8161 tcggtcaacg gacagatatt ggtatctccc ataatgatgg tcgtatactg tactccgggc
     8221 tgggaacgca tctccatcat atggaactct ccatcctgat acgtattgcc acatactgtt
     8281 tctgcctgtc tggaccaatg aacgccttct actccataga tccacaatgt ttccatgtct
     8341 ccatcatcaa aaatgctttc cagaaaatat ttgaatacgc cttccggatt cttacaggaa
     8401 gaagtaatcg catacaccgg tgcacatctc tgccagtatt ttccaacctc tgcgataggc
     8461 ggcataaata ccagttcgct gtcctgtcca ttcgcagcca gtccatccga cagctgatca
     8521 gaccatccgc ctgcccagta agtaaatgct ccgaactccc cttccatgaa tttggtacgg
     8581 cagttgctgg tcgcattcgt cagagattcc ttatcgatat aacccttgtt gtaagcatcc
     8641 tgtaatctct tgatcgcttc cttcatggaa tcctcggtaa atccatctct ccatacgcca
     8701 tcctctccct gatagaaaga aggatatgca tcctgataga attccggcag ataattggta
     8761 taaggagctt catttcctac aaatccggct gcggatacac caatggtatt tccattgata
     8821 ccgtctccat ccggatcacc gttgctgaaa gcatccaaca tttccagata ctcttcatac
     8881 gtcgtcggaa tgtccagtcc acagttatcc agccactgtt ttctgaggaa agtcatacat
     8941 ccgccacctc cgccaagtgc cagtccgtac agatgtccat ccaacatatt gccttctacg
     9001 acactcacat ccgtgttggg attcttcttc agctctgaat tgttccaggc atccgtcata
     9061 tcccagagag ctccctctcc cgcatatccg gaataatagg aagatccaag gataacgaca
     9121 tctggccagt tctccggtcc gctggcaaag gtctgtccca acacatcata gtaggcattg
     9181 tgatccggtt ggatgacttc cagttcgatt cctgtcagtt cttcccattt attgacccat
     9241 tcttcctgac cgtctgccaa tgtcgcctgg aaagttccgt ctaccataat cgtaatcttt
     9301 tccggtttct ctacatcatc tgcaacatct gcaacatccc cggcagtatt tcctgcctgt
     9361 gcatcgctat ctgtctttcc tgtatcattt cccgtatttt ccgcagagct cccacaccct
     9421 gtaaatatca tggaacatac ggtaaccgca accagtgcga ctgataataa ctttttcttt
     9481 accataaata acctccgtcc atattctgtt atcttctctg ttgtttcttg tttcttattt
     9541 tgttttgttt ctttgctgct tgatttgttg attattgtca cttcggcgcc ggaagtatca
     9601 atgtgctttc attatatctg tcattttgca caatttaaag tcacatattt caagatttag
     9661 tgtatatatt ttttgttatt agaggctcat tttttatgtc aatgatacac attttatttt
     9721 attacattcc atttcattgc ctttttcacc tattatgata ctataataat atctctcagg
     9781 agggtttttg ttatgaattc cagaaaaaat aaatacttca taaaattgat aacatcctac
     9841 agtctgctct tggtcattgt gctcattctg ggaattgttt tccattttat tctttcgcat
     9901 aatgcaaagc aggaactatt gaatgaaaat atgctttctc ttaaaaatgt atccgagcaa
     9961 tacagcaact gctatgcgaa tatttatctg atttccaagc gaatggcaaa taattccagc
    10021 ctttcccgcc tggcggaagc cgatcagggg gaacgagcgt tttattataa tgcctatatt
    10081 accaaacagg ctctggtaga tatgtattct gactattcac tccagccaat tcagacctat
    10141 tatatccatc tgcgtcatac agactacata atcagctata atgattttga gaatctctat
    10201 tccttttata aaaacaatca aatgctggat ccctcaaaat atgaggaatg gaaggagact
    10261 ctggaatcct cagacaatta taaccatttt ctttctcttg accattttgc gaccgaatat
    10321 acaccacaca gaaaaaacta ttgttatgct gtctctctgc agaattatac cttcaaaaat
    10381 atcaatgctg acatcatttt tgagctgaat gctgattata tgcagcaaat ggtggattct
    10441 gtcgatctga tgaagaatgg ctgtctcatc atccaggata gcagcagtga cactatgctt
    10501 actttcagta acaatgaagc gctgttaaaa aaatagatgt ttctctgctt tcctcgttgg
    10561 attattccga acggggatat tccagcacag tatttcaggg tgctcccgtc acagtactct
    10621 atacctccgc acagacaccg aatctgaact attatctgat tgttcccacg tcctacctga
    10681 aagcaccggg aaacggactg cagttactgt ctctattcgt tatctttctc ggtgtcgtag
    10741 gaagcttttt gattattgtt ctgttgtcca aggccaatta taaaccctat gaagcaatgg
    10801 aatatcgcct gcgggaagtg aatttcaacc acgaagagct catgcggtta aatgagtccc
    10861 aaaagatcac cctccgcaat tattattttg atcaattgat tcatggtacg attctgagtg
    10921 aggaagaagc tgcgtatgcc caaaattatc tttccatttc cgctaatgcc aattcctttg
    10981 ctatcttata ctgtaatgcc tacctggata ctttggagct aaataatgac cctcagggaa
    11041 tgatcaacat cctctcccct gattatgcgg atgttatttc cgatattctt tccagttatt
    11101 ttatacattc ctatatcatg cagggttccc gaaatagtgt ttatactatt ttattatatg
    11161 acagggaaga actggacaag gtcgaagatt tattttcaca tgttcatact acactattgc
    11221 aagaatacaa tatctggatt tatggtggat taggctgtac aaccgacacc atctcctccg
    11281 tctggaaatc gtatcgccaa tccaaagagg ctgtaaaacg tatctccagc ccagtgtacc
    11341 tgtgtctgta tcgtgagatt ttggaatttc aggattcctt cttttatcct aatgaggttg
    11401 cggatcagct atataatttt gtaaaatccg gtaacttcaa acagacaaaa acaattttta
    11461 attttataaa ggacgaaaat tttaagaaac gaaaacttaa ttccagacaa gtgaaatggc
    11521 ttttagaaaa tatagaaatt acgttattga aagtaatcag ggataacgac atttcctatg
    11581 atgactcaag cttagccggt ctgtccgaag attctaccct gaaaaattac gagcagattg
    11641 ctctggaatt atgtgaaaaa aacaagcaac cggaaaacga caaccagctg attgtgcgca
    11701 ttcaggctta tattgatgag aattatgggg atccatcctt atgtttaagc agaatatcgg
    11761 acctgttcca catctccgag agttactttt ccttcctttt caaaaaaaca gtaggggtaa
    11821 acttctctac gtatctggaa caggtccgca tgacgcaggc aaaacatctg ctggaaacaa
    11881 cgaatattaa aataacagat ttatacgtgg cggtcggtta caacaatctg accagcttcc
    11941 gccgtgcatt taagaaaaaa tacggtgtag cacccaatgc ccttcgagaa taactactta
    12001 ttctttttcg gttgctttct cgcgcagcac ttcctcaaac tgttcgatgg gtaccggttt
    12061 ggaatagtaa tatccctgct gccaggtaga tccaaatttc tcgatatagt cgctgacttc
    12121 cttatcctca ataccctcaa tacaggtttc ggtgtttgtc ctcctggcat aatccacgat
    12181 agactgcacc atggcctgat tctgaggttt ttgcttaata tcacggatga aactcatatc
    12241 taccttcagt tcgtcaaaag gcagctccag cgccagactt aaggaactgt tgcctgttcc
    12301 gaagtcatcc agtgcgatct tgatcccctg ggaatggaaa aattctacct ctccgcgcag
    12361 gaaatgaata tccagatctc tgcatcgttc tgtcaattcg aggcaaagct cctccggctt
    12421 cgcaccggtc tcttttaaaa tattcataac agcactgcgg aactctctgc gctccagctg
    12481 tgccgccgcc acattgacat tgacgaaaaa gccttccgtc tcctcgcgca gcttctgtac
    12541 atccgtaagt gctgtccgaa ggatccagtt ccccagatca aaaatacagg gatcctgttc
    12601 cagccactcc atgaacacac cgggggacac catcccgtaa ggctccagtt cccatcggag
    12661 cagcgcttcc attccccgga tcttgccggt cttcgtatcg gcaatcggct gatagaacat
    12721 ccggaagcct tcaaagttat gcgtagcaca ctgatggatc acaccgatca gttcaaactg
    12781 gttctcgtca ctgccacaga cctcatcatt aaaaatcacc agctctccgt gatgctcaag
    12841 ccgggaatgg ttcaacgcat aagtcagacg gctgcggacc gcgttcgtct ccttcatata
    12901 aggttccata aatattgcgc cggcagatac cttcagaggg atctttttcc cctctacctc
    12961 tgtattttca aaagcttcta cgatctcatc gtatatctgc tgtagttcct ctttggatac
    13021 atttttaaag ctgatcacaa atttcgcacc ggagagccgg tatacatatc cttttcccct
    13081 taacaggcgc aacagctcct gtgccgcaca gttcagaatc ttatccgaaa aagcggcacc
    13141 gtacataaca ttgatatgac taaactgatc cagtccgatc ttcagcagag tcccctgtcc
    13201 gctttcctcc aggaattccc tgatttcccg atccagcgcc ggttccgtat gcaatccggt
    13261 agtcgcatcc acctcgtcgg aaataccgta gttgttatat cgtattacga tcacaaaagg
    13321 ctttccatcc ttatcattca gcattttcgc cctggcactg atccggttgt aggtgcttcc
    13381 gtcacgcacc cggtattccc agggctcgtc catatttttc ccggcaactc tctcctggaa
    13441 tcccctccgg aaatcctcca gatcatccgg atgaatcgat ctttttgatt tttcttgtcc
    13501 gcggatggta tagttctcct gcataccaaa atattccatc gtcttctcgg accaccatgt
    13561 cacgccgttc cgcagattgg ttatggatat gtaggacttt ttcgccagag aagtaatggc
    13621 ttttacaata tccgggaaca ggatatccat ttcctcccag aatttctgtt catcatatgt
    13681 attgatactc atgtctcctc ccgacacttt cttctgtatg cccacagtct ctgaatgcac
    13741 ccttgcatca aataaattat agcatatcga aatgatgttt acgatagtat aatgaatata
    13801 aattttttat gaaataaaat atcttatgat tgacttcgaa aactatctgt aatactatgc
    13861 aatatagcag ttatttcaaa tttgaaatat tcacttttgt aatttcagta attacacttt
    13921 gtataatact aagtaaggag gccttcggat gaatactgat atcgaattcg tacaaaaatt
    13981 caatctggct tacaacgcca tgtgcaaacc tctgtgccag cagttaaagc tgccacagac
    14041 agcttttgat attctcatgt tcttcggcaa taatccggaa taccacactg ccagtgatgt
    14101 cgtggagatc cgccggatca aggcgaatct ggtgtccgtc aatgtggaca agctggtgtg
    14161 cgaggggtac ctggaacgca gagaggctcc ggatgaccgt cggaagactc tgctgttctg
    14221 cacggagaaa gcacaggatg tcatcgaaaa aggccgcatg atacagagca ttttcaaaga
    14281 tactctgcta gatggcaccg atgagacaag caaagagatc tttttccaca tgcttcatgt
    14341 catggagaga aatatggaca atatcctgaa aggagataaa taaatgcaga ttttaatcac
    14401 gattcttgtc acttttttcg ccggcatggg agccggcctg ggcaccggat ttgccggaat
    14461 gagcgctgct gccgtcatca gtcccatgct gatcactttc ctcggcatgg atccgtatct
    14521 ggccgtcggc attgcgctgt cctcggacgt tctggccagt gccgtatccg cttataccta
    14581 tgcgaaaaat aaaaatattg atgtcaaaaa cggcctgatc atgatgggaa gtgtgctggt
    14641 attcaccgtc gtaggcagct acgtatccag cctggtgcct tccgctacca tggggaattt
    14701 ctccgtgttc atgactttcc tgttaggtat caaattcatc gtccggcccg taatgaccac
    14761 caaagaagcc atgcagggcg tctccgcgaa aaaaagagcc attcagtctc tggtatgcgg
    14821 tatgctgatc ggctttatct gcggtttcgt cggtgccggc ggcggcatga tgatgctgtt
    14881 gatcctgact tctgttctgg gctatgagct gaaaactgct gtgggaacca gcgtctttat
    14941 tatggctttt accgctctga ccggtgcagt ttcccatttt gccattgggg gcatgcccga
    15001 ctggaccgta tggatcctgt gcgtcatctt taccctgatc tgggcccgga ttgccgcact
    15061 gttcgccaac aaggccacac ccaagactct gaaccgtgtc accggtgtga ttctcgtgat
    15121 cctgggtctg gtagtaatgg gcttcaacct gttaacctaa gtgatttcct ataccttgtg
    15181 tattctccgg cggagcgatc gttacattgc tccgctgatt ttttataatc tcacggattt
    15241 cagacagatc ctttgcaaaa tagattcctt cctgtctctc cggggaggac agccccatct
    15301 ctgtcatatg tgataacagt gctttcaggc tgtcataata gccgttaagg ttataga
//
LOCUS       DAWEJW010000069        14900 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1457_length_14900_cov_69.084001, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000069 DAWEJW010000000
VERSION     DAWEJW010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14900)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14900)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14900
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_14900_cov_69.084001"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4123 a         3240 c         3759 g         3778 t
ORIGIN      
        1 actaactctc aagttaatat aagacgggtg agctttacag ctcacccgtc ttatattttg
       61 caacgatatt ctgaattctc tcatttggat tcaacagatc gctgatggaa gaatcgtggt
      121 tcaaagtatc aatgatgtaa gcaatgtact tactcatatc acagttgata taccactctc
      181 tctgaagcag ttccggagtc tggtagatca ggttggtggt cagaaccttg tcgataatgc
      241 cgttctcgta agccttgtca aacttgtcca gaccgttggt gaagaggccg aaggtggaga
      301 atacgaagat cttgttggct ttacgctcct tcagagcagc ggcaacttcc agcatgcttt
      361 caccggagga gatcatatca tcgataatga tcatatcctt gccctctacg ctggtaccca
      421 ggaattcatg agctacgatg gggttacgac cgttgacgat cctggtgtaa tctcttctct
      481 tgtagaacat acccatatcc agaccgagaa cgttggcgat gtagatggca cgtcccatac
      541 caccttcatc cgggctgatg accatcatgt ggctggaatc cagctgcaga tcttttacat
      601 tgcgtaacag acctttgata aactgatatg caggctgtac ggtctcaaag ccgtgcagag
      661 gaatggcatt ctgtacacgg ggatcatgag catcgaaagt gataatgttg tctacaccca
      721 tgcttaccag ctcctgcagt gccagtgcgc agtccagaga ctcgcgggaa gttctcttgt
      781 gctgacggct ctcatacagg aagggcatga tcacagtgat acgacgggcc ttaccgccga
      841 cagctgcgat gatacgcttc agatcctgat agtgatcatc gggagacata tggttctcgt
      901 tgccacacag ggaataggtc atggagtagt tcaggacatc tactaacaga tagagatcag
      961 taccgcggac ggattccaga atctgtccct ttgcttcacc ggaaccgaaa cggggtactt
     1021 tggcatccag gagataggat tctctctgat aacctgcaaa tgccagagac tctttgtgtt
     1081 cgctttctct ctctgctctc cattttacaa gatactggtc taccttctca cctaaaggct
     1141 tgcagccggg cagggaaata atgcccagag agccgacggg gatagtctcc agatttcttt
     1201 tttcttcacg attcgccatt ggggtgatcc tcactttctg attctgtata ctgttctgtt
     1261 acaaacaaat gattaattgt cagatgtatt ctgcatacgc tttttgcaca tacgaatatc
     1321 ggggccggtc agcttacaca gatcgtaatg actggtgata cgggaaaata cccggtctga
     1381 ataccggtcg cgcagttcct ccagactcaa atttgtggag atgatcgtag cgtttttccg
     1441 cagatgtctc tcattcagac aggagaacag ctgggaggct acaaaagtat tggtcatctc
     1501 tgtgccgagg tcatcaatga tcagcagatc gcagttatac agatcctcgt agaatccgga
     1561 gagagcttct tttgccctgc tgtcgaaggc ataacgggca agagtatcaa acagcccgga
     1621 agcactgaaa tagatcacgg aatgaccggt ttgcaataat tcactggcga tacatcccga
     1681 caaaaagctt ttgccagtac ctactgtacc ataaaagaat aaattgtggt agtcttgttt
     1741 gaagttttgt acaaaattgc gacagacatc cacgcatttt cggaaatgcg taagatcatc
     1801 gccctgatag tattcgtaag aaaggttgga aaaattctca gaagcgatca tatcccggat
     1861 atgggactgc tcatagagaa tatccagttc ctgctggtga aaacagtgac attttttgcg
     1921 aagaccatct tcactgtcaa tataacctgt atctttgcaa tcggggcagt catagacggg
     1981 ttccagatag tctgctgggt atccggcgga cgtgagcaat tctcttttct gacgggagat
     2041 ttcctttaga gaagaacgca gtctggtaag cgcctcgtca tcaccgttca ataaaagctt
     2101 tgcctgcgcc acggaaagtt caccgacaga ctcatccagt tttccgtatt ccggaagctt
     2161 tgtataaatt tcccggcggc gctgctctgc caggtgacgg ttgcggtctc tggtctgctc
     2221 atagcccttc ataatagaat tgtattgtgc gttagtcagt gacacggtcg gttcccctta
     2281 tctgctcatt gctggttgtt caatatctga atattagtta cttaaaattt ccttttccag
     2341 agcggcgaaa tcgtagctgt tctgggtaaa ctgtgtaaag cggttggagg acccggaaga
     2401 agcgggcttc gcagtctttt tcttctggaa agagtcatcc atctgctgaa tatcggcctt
     2461 gtggtgtaca ttagcctgtc tccagctgtt caggattcct tccgcatatt caaagcgatg
     2521 cttgtccgta gccagagagc tgcgctcaca ggcttccagt ataatatctg tgctgaaacc
     2581 gtaatctctg gtccaacggt tgatgaattc cagttctttc gctgtgggag aggtactgcg
     2641 tcctaagctg ttcataatgg aatatacgct tctgtcgtag cgggtggaga atttctgcgc
     2701 ctgcttcggg gtggtgattc cttcttccgc ccagttgaca gcaacttttt ctatgtattt
     2761 gaaatctttc ttgccgcgct ccacacagta ctgtagcaga taatcgatca gatcctctga
     2821 aaaatgcagt acatcggtga aaaacaggat cgtcttgatc tcggaaggag tcagaggctt
     2881 gccaatataa gcttctgcaa taaagagaag ctgggaggtc tcttcccgtt ccttgaattc
     2941 ccgtaactga tccagagaat aagccggctt tgcgtaagga ttgacagttt cctggggtgt
     3001 tgtcggtaca actgcagccg aaacctgcgg aatagcagcg gcaggaatca cagagactcc
     3061 ggcgggagta ttctgcatct gcgaaggacc ggaggtcagc aggacattat gctggggggc
     3121 aggagcagcc tgcgtgctca gatcacgaag atgaatccct accagtgttt tattatcatc
     3181 gaagtccaga tccaggatct gctgcttttc ccagtatttc agtgcacgga ttacatcctt
     3241 ttccgtgtgg ttgaacttgt ccgcaattcc ggacacacta atagcctgtt ccgcattcag
     3301 catgcgcagc agatagaaat agaccttcag ctgtgcgtcg ttggcatctt tcatatattc
     3361 atcaataaac agattggata ctaccgtaga atctgcataa ttatctttat agagcgctat
     3421 ccgtgccata acacacaata ccttctttca caaatcgttc ctttttcaaa gtaagaccaa
     3481 gtatagcata agacctgtga aaaagaaata gtcaatttgc cggaaaacct tgtaaacact
     3541 ggactttcgg actcctggat aaaaatgtgg aaacggtgga tattgtggat accaaaagac
     3601 ggtgtatcga gaaaacccta tatatcggca gatcatcgcc aaaatccgac agaaaattat
     3661 ccacaggaaa acaacaaaat atccacaggg aaaacgggga taatccccgg tgtttcctgt
     3721 ggataatgtg gacaactatt tgccaagcag gttttcaccg atttttacta catctccggc
     3781 acccatagtt atcaacaggt caccgtttat gcaattttct aataaaaagt tttcaatctc
     3841 atcaaaagaa ggaaaatagt aacattcatg gcccagtgcc cggatctctg tctggagcgt
     3901 ctcagaactg attcccaggg tatcggtctc acgggcagca taaatatctg ccaggacgac
     3961 tttgtctgcc agacttaagg ctttggcaaa atctttcatg aatgccttgg ttctggtgta
     4021 agtatgtggc tggaagacac accagagggt cttgtgggga taattcgctg ccgcatgcag
     4081 ggtggctgca atctcggtgg gatgatgtgc ataatcatcc acaatggtta caccgccgat
     4141 ggtgcccttg tattcgaatc tgcggtctgt accggtgaaa ccgtgcaggg cagaagcggt
     4201 aacctcgcga tcgatcttca gcaaatcggc cagggcaatg gaagcaatgg cattgcatac
     4261 gttatgctct ccgggaacct gcagagaaaa actgccggac acttccccat gccggtgaag
     4321 cgtaaaggaa ggatgaccga actcgtcata agtaatatcc gagggataat aatcggcagc
     4381 ggacccgtca ctgttgaccg cttctttatt aaaagtgatc acgcgacagt ccagatctcc
     4441 tgtgatctcc tgtaatctgt caatggcacc gttgatgatc agacagccgt cagcgggcag
     4501 aagctttgca aatttatgga aggaattccg gatatcctcc agatccttga aaaagtccag
     4561 gtgatcttct tcgatattca ggatcatacc gatcctgggg aaaaagctta agaagctgtt
     4621 ggtgtattcg caggcttctg taacgaacag atcggtactg ccgacacgga tgttgccacc
     4681 gatgcttttt aacattccgc cgatggacag agtgggatcc gtaccggcat gcagcagaat
     4741 ctcactgacc atggaggtcg tggtggtctt gccgtgagta ccgctgatgg caataggggt
     4801 cttgtaattc tgcatgatct ctcctaaaag ctgcgctctg gtgagatgcg ggatcccttt
     4861 ttccatagta gcgacatatt cgggattgtc tttgtgaatc gcgctggtaa atacgacaca
     4921 atcgatatca tcggtaatat tcccggcacg ttgtccataa aatacattga tcccctttct
     4981 ttccagagtt tcggtgaggg ggcttttgga acggtcggaa ccggacacac ggaagccggc
     5041 atccttcaga atctctgcga gaccgctcat gctgataccg ccgataccta caaaataaat
     5101 ggacgagggg tgattaaagt ctatctgata catattggca cttccttaat atagtgtagt
     5161 tgcattgctt attatgtaac tattcagagc agttacctta ttattaatat tatatacata
     5221 ggaacgaaaa aaaacaatcc ataggtacga ttttgaaaaa atgtgtaagt ttactatttt
     5281 ttgcaaaacc aatccagacg ggattggcat aaattgacaa aaagcttaca aaagataagc
     5341 taattatgta aaaaatggat atgaaaatgg taaaatgaca aaaaaaccta gtaataaata
     5401 ttcactttcc tttggcaatg tgttataatc agattatcac atccgaagga aagagcaggc
     5461 actttttcga gacagatgga tacatcggat aatagccgcg agcgactatt tattacaaca
     5521 ggtggaaggc tagatagagg tgaggatatg attaagaaag aaatgatagc catgctattg
     5581 gctggaggac agggaagccg tctgggtgtc ttgacatcca aggttgctaa acctgccgtt
     5641 gcatttggag gcaagtaccg cattatcgat tttccccttt ctaattgtat taattcggga
     5701 gtggatacgg taggcgtact gacacagtac cagcccctca gactgaatac ccatatcgga
     5761 atcggtattc cctgggatct ggatcgtaat atcggaggag tgaccgtgct gcctccctat
     5821 gagaagagta ccaacagtga atggtatacc ggtactgcga atgcgatcta tcagaacctg
     5881 gagtatatgg agagctataa tccagagtat gtgctgatcc tttccggaga tcatatttat
     5941 aagatggatt atgaagtgat gttggatttc cacaaggcca acaatgcaga agttactatt
     6001 gcggtaatgc ctgttcccat ggaggaggcc agccgtttcg gcatcatgat cgcagatgag
     6061 aaccatcgca tcaccgaatt tgaagagaag ccggaacatc ccagaagcaa tcttgcaagt
     6121 atgggtattt acatatttaa ctggaagaca ttgaaggaag cactgatcgc tatggcagat
     6181 cagccggcac tggattttgg taagcatgtc attccttact gccatgaaaa gggggctccg
     6241 ctgtatgcgt atgagttcac cggttattgg aaggatgtag gtactttgag ttcctattgg
     6301 gaagccaaca tggaactgat cgatatcgta ccggaattca acctgtatga ggaatactgg
     6361 aagatctata ccaagagcga gatccagccc cctgattata ttgctgccga cagtgtggta
     6421 gagcgcagta tcatcggtga aggctccgag gtctatggag aagtatataa ttccgtcatt
     6481 ggttgtggtg ttacgatcgg taaaggaacc gtgatccggg attccattat catgaaccag
     6541 acccagatcg gtgaaggatg tgagatcaac aaggcgatta tcgcagagaa tgttgtggta
     6601 ggtaatcagg taaaattagg tgtcggtgaa gaagcagaga acgatacggc acctcatatt
     6661 tacaaccacg gacttgtaac catcggtgag aagagcgtga ttcctgacgg tatcagcgta
     6721 ggcaaaaact cagtgatatc cggagtgaca gctgctgcag attatgagga ttcccagctc
     6781 gcaagcggca aaacattaat taaggcaggt gaatagcagt gagagcgatt ggaattattt
     6841 tagcaggcgg taacaatcac aggatgcggg aactttccca gaagagagct atatgtgcga
     6901 tgccgattgc aggcagctac cgcagtattg actttacact gagtaatatg accaactctc
     6961 atattcagaa ggtggcagtg tttacccaat acaatgcaag aagtctgaac gagcatctga
     7021 attcttccaa atggtgggat ttcggacgta aacagggtgg attatatgta ttcactcccg
     7081 ctgtaacagc agagaacagc aactggtaca gaggaaccgc agatgcaatt taccagaatc
     7141 tgcagttttt gaagaacagc catgagcctt atgtagttat cgcatccggc gactgtgtat
     7201 acaagctgga ttataataag gtgctggaat accatatcga gaaaaaagca gatatcactg
     7261 tagtctgccg cgatatggaa gccggtacga atgtagagcg tttcggtacg attcgaatga
     7321 acgaggaaag tcgtatcgaa gagttcgagg agaagccctt acaggcgaag accaatacca
     7381 tttcctgtgg tatttatgtg gttcgacgca gactgctgat cgagatgatc gagaagtgtg
     7441 ctgaggaaga ccgctacgat ttcgtaagag atatcctgat ccgttacaag gatctgaaga
     7501 gaatctatgg atacaagatc aaggattact ggagaaatat cgcatctgtg gatgattact
     7561 acagcaccaa tatggacttc ttgaaagcag atgtgagaaa ctatttcttc aagcagtatc
     7621 cggacgtata ttccaaggtg gatgacctgc ctcccgcaaa atataatccc ggttcccagg
     7681 tgcgtaacag cctggtatcc agtggatgta tcgtcaacgg tgtggtagag aattccgtga
     7741 tctttaagaa gagctatatc ggcaataatt gtgtgatcaa gaattccatt atcctcaatg
     7801 atgtgtatat tggagataac acgcatattg aaaactgtat tgtagagagc cgtgacacga
     7861 tccgtgcaaa ttcgtacttt gtgggagaag gcgaagttaa gatcgtagtt gagaggaatg
     7921 aacgatacgt gatctaagtg atccggattc tcaatgacta cattgcgaga ctgcttgcag
     7981 gcagggcaat gtggtcatta gggaatctaa cagatatgag taagtagggc gatagcccgg
     8041 aatacgaata tctgtagcgt ttcgagagcc tgcagggcgg agaaatgcgt agggatcacg
     8101 ataacaccaa gggggttagc aaatgcagat tacagatgta cgtgttcgta agatcaccaa
     8161 ggaaggcaag atgaaagcta tcgtatccat tacactggat gatgaattcg tcgtacatga
     8221 tatcaaggtg atcgaaggtg aaaagggatt gttcattgca atgccgagca agaaggcaac
     8281 ggacggggaa tacagggata tcgcacatcc catcaattct tccaccagag agaatttgca
     8341 aagggtgatc ctggagagtt acgaaaaagc attggaggag ccggaagagg caatctaagc
     8401 tgcataggga taaacaacaa aagctgttgt caacagagtg atctgtggca acagcttttt
     8461 aaattgcagt tatcttttat tccccggacg aaaatcagga acgctgataa gcacataatc
     8521 cgttttcaaa ttgcagacga tcgatcaatt ccagggaacc gccgattcgc cggtatagtt
     8581 ctatggtgcc tgttgcattg ccgccttccc agagaggaat gtcggatact tttccgtcag
     8641 gactttcata gagtaacggc atcatctgct ctttgctgca gcttccggag atccgtacaa
     8701 cggaagtgcg gttctgggct ttgaaatgcc agataaaacg tttgttggtc tctttcactt
     8761 tccacttgca gcgggacagt gtaagaggtc tgccaaaatg atactcgaaa tcttccccgg
     8821 tataggtcag ctgcatcaga atcctgcgtc gcatggggaa aaagagaaat ttcggacaac
     8881 agccgtcgat ggccagagcc gaatgcttca attcccgccc ggttttctca ctgatcaggt
     8941 ggccggaagc aaattgcagc cagggctggt tgtaactgcg gccccaatgt ttgtccgcat
     9001 agccgtagga tgtctcggga gttacctcgt aggtgacacc gttcaaaatg acggtcccac
     9061 ggaaaaaggt acggatacct tccccatgcc aaaaactttc cagggcatga accgcggtaa
     9121 aaaaagcatt ggcaatatag ccggtatgac aggcaaccgc cttatgcact tccagatccc
     9181 agataaaact gcccgcatcg gtcataagag agcgatgtct tgcaacctca tcggagatat
     9241 cgatcgaacc actgatccgg ttttcgctgt agacacaatc ctcaaactgc atgtaaaagg
     9301 gatcctgggc cacctgcaga gaggtcaagg gataatatgc ccgaagctgg agcccgctat
     9361 ctcccggctc agggaataca ccggctttta tgcagagata agatggtttc agaccatgac
     9421 gtttatagta gggatgctga cctaaaatag gctgggaagt accgagagcc ggatttaaaa
     9481 tgctgtattc tacaaaaaaa gtgcgggttt cctgcgtcgt gggacaaatg ccctggaagg
     9541 aatgccacca gcgcatgtag ccacgttttg catcggaacc ccgcaacata tatgcatttc
     9601 ttttcagatc agataaattc atggatcaaa gactcccccg cttttgtacc cctcaaaaca
     9661 acatttcgta gataatatgt atattatagt actatatgtt gtgaatgaca agagtgtcag
     9721 aagaatctta agaattattt agatattttc tttgatccgg atatctaccg ctacataatt
     9781 gtattctttt tccggatttt cgccggtcgt cagataggca aacagcaggg aaagagggtt
     9841 gccgccctga attttcggct gttggcagat ggtggcggca atggtgccat tttccagcat
     9901 ttggcgtgtg gtggggacca gatcaaaagc gatgatccgg atcgcctttt ttttacccag
     9961 agcctctaca ctgcggcagc ctccgtagac accgccggcg gcgaagaaca gtgcattgat
    10021 ctccgggtgc tttgacagaa gtgtcgttgt cttttcgtag ctttccactt cgtcatcgtg
    10081 gatctccacg gtatccacga cgcggatccg atcagagaaa ggttctaatg tcttcagaaa
    10141 tcccgcaatg cgctctgtgt gacagagaat gttggaggaa cctgtgacaa tacccacgtt
    10201 taccgtgccg gaggtcatca attgcagaag accggcggcg gtggcaccac tgcgggtgta
    10261 gttgctgccg acataagcga tacgtctgga attttcaatg tctgtattca gcgtgaccac
    10321 gggaatcccc tgcccgtata aagtattgat gcagtcgcgg atccgttcgt cgttgtaagg
    10381 ggtcatggca atgccgttca cttcttctgc aagaagctct ttgactgcct gtaactgggc
    10441 ctccacaccg aaagaaatct gtttggtgat cacggtacag ttgtaacctg ccagctcttc
    10501 cgccttttca ttgattccgg tcagaacatc ctgaaaaaaa ggattcccgg tggaaaacag
    10561 aatgacaccc agcttcagcc gctttttctg cgctgccaga acgagaccgg cgcggttggg
    10621 tttatagtcc agctcttttg caatttttct gatcttttcg gcggtctccg ggtttactgc
    10681 gccgcgatcg tttaagacgc gatccacagt gccgcgggat actccggcca atgcggcgat
    10741 ttctttgatg gttgccatag aatactcatg cctttccgta atatctgttc caggtatctg
    10801 gttataaagg tatcatattt ccagagggac gtcaacgaaa gtgcagaatt tgtccgggca
    10861 cgctgaatgg atgatgaatg atgaaattcc atcttcactt tttagtgaaa aaatactgaa
    10921 aacataaaaa cttctacttg aaaaattttt tcacaacagt tatgataaga ctataacgtg
    10981 cacgggcacg aaaaggaacg aaaatggatc acaacacaaa cgtaggagga agattcatgc
    11041 tatatattgg aatcgatctg ggcacttcgg cggtaaaact gcttttaatg gacagtgaag
    11101 ggaacatccg gaatattgta tcccgggaat accctttgta tttcccacat cccggctggt
    11161 cggaacagaa gccggaggac tggtatgaac agactatgga gggcatccgg gagcttttga
    11221 aagatgcgga caagagccag gtggcaggca tcagcttcgg aggacagatg catggactgg
    11281 tcattctgga tgagaaggat caggtgatcc gccctgccat cctctggaac gatggcagaa
    11341 ccacagagga atgcgattat ctgaataatg tgatcggcaa agagaagttg tctgagtaca
    11401 ctgcaaatat ttctttcacc ggatttaccg cgccgaagat cctctgggtg aaaaagaatg
    11461 agccagagaa cttcgcacgg atcaaaaaga tcatgttgcc gaaggattac atagcttatc
    11521 gtctgaccgg cgtacattgt acggatgtat cagatgcttc cgggatgtta ttgtttgatg
    11581 tgaagaaccg ttgctggtcg aaggaaatgt gtgagatctg cggagtgaga gaagagcagt
    11641 tggccacttg ttatgagagt tatgaaaagg tgggctgcgt attgccggag gtggcagacg
    11701 aactggggat tcccgcaaat gttgtggtag cggcgggagc cggggataat gcggccgccg
    11761 ctgtaggtac cggaactgta ggagacaata tgtgtaatat ttccctgggt accagtggaa
    11821 caatctttat ttcctccaat catttcggag tagatcagca caatgccctg cattcctttg
    11881 cccacgcaga cggacattac catctcatgg gctgcatgct gagtgccgct tcctgtaata
    11941 aatggtggat ggatgagatc atcggaacga aggattatgc cgcagaacag gcgcagattg
    12001 agaagctggg agaaaatcat gtctacttcc tgccgtatct catgggcgag cgttctcccc
    12061 ataatgatcc caatgccaga ggaactttta tcggaatgac catggatacc accagagccg
    12121 atatgacgca ggcggtactg gaaggcgtgg catttgcact gcgtgattca ctggaggtgg
    12181 caaaatctct gggaatccat ctggagcgga ccaagatctg cggtggcgga gccaagagcc
    12241 cgttgtggaa gaagatgatc gccaatatcc tgaatctgaa ggtggatgtg atagaaagcg
    12301 aagaaggacc ggccatgggc ggtgccatgc tggcggcagt agcctgtggg gaatatgcaa
    12361 gtgtggagga gatcgcggag aagttcgtca aagtcaccgg aacggtagaa ccggatccgg
    12421 aactggtggc aaaatatgaa gaccgctatc agaaattccg ccagatctat ccggcatgca
    12481 gaccgctgtt tgagatcatt aaataacgcg tagaaaaatc aaacaacaac ataataaaat
    12541 gaacctcgta attttaagtt tgagctataa tagagaaagg ttggtagagt agaatgcata
    12601 ttttatctgt aaaagatcct gcatttcgta agtatggcag agtgatcacc aatgtggatt
    12661 ttacacagct ggtggaagaa atgaaaaaga ctccggttcc tgacggcgtg gtgtacgagc
    12721 ccgggatcaa tgctttggaa gcattacctg tgaaaaaaga actggagacc atcacatacg
    12781 gagaaatgcc tgtacagatc ggttactgca atggccataa ttccaaactg aatgccgtgg
    12841 aatatcacag aagttccgaa attaatgtgg cagcaacgga tgctatcctg atcctggggc
    12901 tgttagcgga tgtggcagaa gattttacct acgatacttc taagatggaa gcatttttag
    12961 taccggccgg aaccggtgta gaggtcttcg caacttctct gcattatgct ccctgtggag
    13021 tagacggaca gggattccag gtagccatcg tgttacctca gggcactaac tatccgttag
    13081 aaggagcaca tcagaaggtg gagcagggca aggcacccag cgaggatgcg ctgcttgccg
    13141 ctacgaacaa atggctgatc ggccatgcgg agggtggact tccagaggga agcttcctcg
    13201 ggcttgttgg agagaatctg gatgtcagca aataatggtg gcagaataaa aagatttgaa
    13261 cgccggaatc ccctgcatgg atgtatgacg gcggtaaaat aaatggtgta gagaaaaaga
    13321 aaacgaaaat aggaggatta caaaatgagt aatttacccg aagtaaaaat tggcgttgtg
    13381 gctgtcagcc gtgactgttt ccccgagagt ctttccgtga acagaagaaa agctctgatg
    13441 aaggcttata ccgagaagta tggaacagac agtgtctatg agtgcccggt atgtatcgta
    13501 gagagcgaga tccatatggt acaggctctg gaagacatca agaaggcagg ctgtaatgca
    13561 ctgtgcgtat atctgggcaa ctttggcccc gagatttccg agaccttact ggcaaagcat
    13621 tttgacggtc ctgcaatgtt cattgcagca gcagaagaga cccagaatga tctgctaggc
    13681 ggcagaggcg atgcttactg cggtatgctg aatgcaagct acaacttaaa gcttcgtaat
    13741 gtaaaagctt atatccccga gaatcccgta ggaactgcag cggaatgtgc agatatgctg
    13801 catgagttca tttctatcgc aagagctatt gtgggattaa gtgatctgaa gatcatttcc
    13861 ttcggcccca gacctttgaa cttcttagct tgtaatgcac ccatcaagca gttgtacaat
    13921 ctgggggtag agatcgagga gaactccgaa ctggatctgt tcgaagcctt caagaagcat
    13981 gatggagacg agagaatccc cgccaaggta aaagagatgg aagcagaact gggggcaggc
    14041 aaccataagc ccgaagtact gcctaagctg gctcagtatg aattgacact gttagactgg
    14101 atcgaggctc acagaggata tcgtaagtat gtagccattg ccggaaagtg ctggcctgca
    14161 ttccagaccc agtttggttt cgtaccctgc tatgtgaaca gccgtctcac cggtatgggc
    14221 atccccgtat cctgtgaagt agatatttac ggctgcttaa gtgagttcat cggaacctgc
    14281 gtcagcgcag ataccgtaac tctgctggat atcaacaaca ctgttcccga tgatatgtac
    14341 gaagagtcca tcaagggtaa attcaactat acccacaatg acaccttcat gggattccat
    14401 tgcggtaaca ccaactccaa gaagctgacc tcctgcaaca tgaagtacca gatgatcatg
    14461 gcaagaaccc ttcccgaaga ggtaacccag ggtactctgg aaggagatat tgttcccgga
    14521 gacatcacct tctatcgtct gcagtctacc gcagacaaca agctgcgcgc atatattgca
    14581 cagggtgaag tacttcctgt agctaccaga tccttcggtg gtatcggtat tttcgctatc
    14641 cccgaaatgg gacgtttcta tcgtcacgta ctgatcgaga agaacttccc tcaccacggt
    14701 gctgttgctt tcggacactt cggcaaggct ctgtacgaag tattcaagta catcggcgta
    14761 cctgtagagg atctgaattt caatcagccg aagggaatgc tgtatcctac cgagaatcct
    14821 tttgcataaa aagaacttaa gtaactggaa agttagttaa atacattggc aaaatccacg
    14881 aactcccgga gcatgcgata
//
LOCUS       DAWEJW010000070        14687 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1488_length_14687_cov_69.137507, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000070 DAWEJW010000000
VERSION     DAWEJW010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14687)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14687)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14687
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1488_length_14687_cov_69.137507"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3367 a         4037 c         3121 g         4162 t
ORIGIN      
        1 cagaaaaaag acagagccgc gaaatggctt cgcatcctct gtcttagtat tttttccgtg
       61 atccttcttg cgtttctctt ctttactggt gcgttcttat ttatacaaaa tgaagcaaat
      121 ttagacgaag cactcttcac cctggctttg ggtgcctttg ccatcgggct tttgcttttc
      181 cgggacggga ttccgatcct tctgcttgcc atcgctctac cgccgttact ttgcagacgc
      241 ccccagcagc cggcgaagac catcgtctgt atcctgctgt cggttctgtc cgtcctctgg
      301 ctgatctttg gcggacactt tctctggttg cagttattct agcctttcca tcgttttgca
      361 taatctttcc gtccgctgac gatggcagaa taagcatcag aagaatttta ttcctaatta
      421 ctttccaaac agtgtactca aaattcctct cttgatgacc tgggcaccgg tgttaatcag
      481 ctggcgctcg atctgggcct gtctgcgttc cgctgccttt tccttctttt tctgtgcggc
      541 ttcccattcc ttctgcttct gctttacggt ggattcctgc agttttgcct gctgctttgc
      601 cagagcttcc ttatccttct ccagctgtgc ccgctctgct gccagctgcg ccgctttcgc
      661 ctcatgctcc atggtatctg tcaacacttc gtaggcagaa atattgtcca ccgcctcatc
      721 gtagcgtccc atgccgtctc tcgccatcag attcctgcgc tccgtctcat ccacaggtcc
      781 cataaagctc tgggggcaga tgatggaggt acgctgggca atgccgggca cgccgttctc
      841 atccagaaag gaagtcagtg cctcacctac gcccagttcc atgatgactt cttctgcctt
      901 aaaggcagga ttggcccgca gggactgtgc tgctgcccgg acagctttct gctccgccgg
      961 agtgtacgcc cggagggcat gctgtacccg gttgcttaac tgtgccagca cggaatcggg
     1021 aatatcactg gggctctgag tcacgaaata gatacccacg cccttggaac ggatcagctt
     1081 taccacctgc tcgatcttct gcaccagtat cttcggtgca tcagcaaaca acaggtgtgc
     1141 ctcatcgaag aaaaatacca gcttcggctt ttccagatct cccgcctcgg gaagcttctg
     1201 gaataattct gacatcatcc ataacagaaa gctggcatac agtgtgggat tctgcaccag
     1261 ttttacgcag ttcagcacat tgacaatacc tttgccgtac acatccgtac gcatccagtc
     1321 ataaatatcc agatccggtt ctccgaaaaa gagatctccg ccctgattct ccagaggcag
     1381 tagcgcccgc agaataccgc ccacggactg cttggagata cttccgtagg tggtcagata
     1441 gtcatccttg tgttctgcca cataattcag cagaatacgc agatccttta agtcgatcaa
     1501 taaaagtccc ttgtcatccg cgatccggaa gacaatattc aatacacctt cctgcgcagc
     1561 ggtgagcccc agaatacggg ataaaagctc cggtcccata tcggagatgg tggctctcac
     1621 cggatggccg ccctcctgat agatgtccca gaagcagacc ggataagcct tataggaaaa
     1681 actgttgcgg ataccgaatt tatcaatcct cctgtccatg gattccgaag ccacaccggc
     1741 tgtggcaatt ccggaaacat cccccttcac atcacacaaa aataccggca ctcccgcatc
     1801 tgaaaaagat tctgccatag ccttcatagt gatcgtcttg ccggtgccgc tggctcctgc
     1861 gatcagtcca tggcggttac tcatattcag cggcatattc acacgctgac catctgccag
     1921 gcccatatag atctgtccgt ctgtatacat aattgtccct catcctctct attttatctc
     1981 tattttcctg ctgccctatc ccgtagggat atcaatagaa gatatcttct acgacatttt
     2041 gaaacgtttc ttccccgtca tatgctccac tttcaggcag aatatctccg tcatggctac
     2101 gacttccggt cggaatacga aattctccgt tctgtcagga tagtactgct tcatcagaag
     2161 attcaatgct ttctgttttt ccgcatccga caggatctct atagttccct gaccgatcac
     2221 gctctcgtat tccatggtgc actggcagcc atcttctttc tccgacagca ccagtctgtg
     2281 ggagcaatcc atctcgaagc ttgccctgac atccttacgg atcaagtcat atttttttcc
     2341 ttcttttgcc ccgtgaaaat agagtgtcac tctgccgtcc gtctcttcca ttccgaaatt
     2401 caacggcaga agatacggat agccatcctc atcattcagt gcaagtctgc acacatcaca
     2461 cctcttcatg acatctgtca tttccgaaaa ttctgtaatc tcacgatcgt tacgacgcat
     2521 tgtattcctc ctgtgctgca ttttgttttt ctgtcaatat ctgtgcgccc gacaggcttt
     2581 gcggcatata aatgccgggt aggccatcat tggaatttca tatactcttt cacagatata
     2641 tttactatag cgcataaaca agatataatg caactatatc cttccgtaag gaattttgaa
     2701 aggagtttta ttttgcaaaa caaagtatcc cattgttcta ttttcccacc ggatctcagt
     2761 gctctgacgc tgcatatcct cgctatggcg ttcatgttct gtgatcacct gtgggctacc
     2821 ctcgccggag atgcctggtg gctgaccggc atcggcagac tggcatttcc catgttcgcc
     2881 tttttcctgg tggaaggctt cttccatacc catgatagga aaaaatattg catgcgcctg
     2941 cttcttctgg ccattctttc tgagcttccc atcaatctga tgtacagtgg gctgttattc
     3001 tacccgtttc atcagaacgt gatctggaca ctgctcacag gcttcctatg tatctgggca
     3061 atagataccc tacggaaaaa atgtccggtc tggctgtgga ttccgtcgat cctgctgcta
     3121 tctgcagtgg gctacgtgct ggcaaccctc ttcatgttcg actattacgg agaaggcgtt
     3181 ctgactgtta tcgtattcta tctcttccat gggaaaaatt ggtggcagct tgccggacag
     3241 tttgccgggc tctactggat caatgtcatg ctgttggcag gaatgcagat tccattacag
     3301 cttttcggtc atgccttcga gatttcagaa cagggactgg cgctgttgtg tctgccgctt
     3361 ctgtggtgct accatggacg ccagggcgcg cataaccgta agatccagct tgcctgctat
     3421 gccttttatc cggtacacat gctggtactg gggattctgt caaagctgat ttttagttaa
     3481 aatgataatg ctgacagaaa gtctattccc taaagtaaaa aactcccgat ggaatcattc
     3541 caccgggagt tctatatttt tactctaaat tagtactgct tcacctgttc ttcgccgttc
     3601 atgacacgca gagcgccctg agccagagct aacagctcgt cctcaccggg atatacggtc
     3661 acaggagcga tccagccgca tctctctttc agcttagcca ctacatcctc accatatgca
     3721 atgcccccgg tgataacgat ctggtctacc ttgccgttca gcacacaggc catagcaccg
     3781 atgtctttgg ctacctgtaa caggaatgcc tgcttcacct ccgctgcctt ggcatcgcct
     3841 tcattggcac gcttggtaac ttcacgcata tcgttggtac ccaggtaaga attcagtccg
     3901 cccttgccga tcagctttgc actgatctcc ttctcggtat attttccgga gaagcacatt
     3961 tttaccagtg ctgcacaagg cacaccgcct gcacgctccg gagagaatgc accatcaccg
     4021 tcaaatgcac tgaatacgtc tacaaccttg ccgtcctcat gtgcgcctac ggatacgccg
     4081 ccgcccatgt gcacgactac cagacgtaag gactcataag gcttgccggc ctcttttgca
     4141 tatctcttgg caactgcctt ctggttcagg gcatggaaga tacttcctct ggggatctct
     4201 tccagaccgg aatatcttgc gatgggcatc agctcatcta ctacaacggg gtctacgatg
     4261 taagagggaa cgccgatctc atcaccgatc tctcttgcca gaataccgcc taagttagaa
     4321 gcatgctgac cctgtacacc tacttttaaa tcctccagca gatcatcggt taccgcatag
     4381 gtaccactgg ggatgggctt taacagaccg ccacgtccta cgatcacgtt gaagctcttc
     4441 agatcgcagt tcttctctgc cagcagatcg gtaatgatct tcttacggaa atccttctgt
     4501 tcgaaaatag tatcgtattt ggagatctcc tcggtagaat gtctcagggt ttcttcaaat
     4561 aataagttct catcctcaaa tacaccgatc ttggtggagg tggaaccggg gttgatgatc
     4621 agactcttga ttgccattgt gtgtttcctc ctatattcac tcttatattc gctcttatgc
     4681 tttaccgctt tgtccactac agcatctata cctgccgaca gcagttctcc cgcagcttcc
     4741 acagcggctt cgatcacgat atccatagct tatttctgat tcttttgcat ctcttctgct
     4801 acaactgcag ccagtgccag ggagtatacc ttggtctcga aggtatcgga acggctggtc
     4861 aggactacgg gtaccttggt accggtcagg atgcatccgt tctttacttt taccatatgt
     4921 acgattgctt tgtataccag gttgccggca tggatatccg ggaacagcaa tacatctgca
     4981 tcaccctgga tcttacggtc ggtagctccc ttatgcttgg ctgcctcggg atcgatcgcc
     5041 aggtcaagag acagcgggcc gtctacgata cagccgggga tctgtccttc ttcacacatc
     5101 ttagtcagtt ctgccgcatc tacggtaacg ggcatcttgg gatttacaac ctcaactgct
     5161 gctaaaggtg ctaccttagg ctctgcaacg ccgcaggcat tacataccgg aatggtgttc
     5221 ttgatgatct gaaccttctc ctccagtgta ggataggtca tgaatgcaac atcggtcagg
     5281 aacagaagtc tgtcaatgcc gggaatctca aatacacata catgagacag ggttccaccg
     5341 gtacgaaggc ctacttcttt gttcagtacg ctctttaaga agttcttgga gtcgatcagg
     5401 cccttcatat acatatctgc acggccgtca tgtgccagct ttacagcttt cagggaagct
     5461 tcgatcatat ccggctcatt gatgatctca taatcggaga ggtccatatt cagtccggct
     5521 gcgatctcac ggatctttgc ctcatcacct accaggattg catctgcaat gcccagtctc
     5581 ttggcctcat gtacagcttc cagtactgca ctgtcctgtg caacggagac tgcgacggtc
     5641 ttcttcccac actcttttac ttttgctacg atgtcatcaa aattcatttt cttccattcc
     5701 tttccgcccc tcgtgggctc caagtatgaa tacattccac acattattcg caaaatcgca
     5761 cgataaatcg tgctttcgct gctgcgcagc tatttttgct catatgtgtg gcggtgactc
     5821 tattcgagtc acctaaaatc aagcaaaact gctttttaca tgcaagcatg acaaagcagt
     5881 cccgcttgat ttcattgtct tccgaatagt cactctaatc gttaaaataa taacacaccg
     5941 gaatacaaaa atcaagctac tatgacaaat atagccacct ctaaatgagg tggctgatat
     6001 ctgccagggt cggtacgcag gcgtacagac atttttttaa tttttcatct ggctgtgtct
     6061 gatccggcac catcacgacc ttacagccgg cgcggtaagc actcatgact ccgttgggag
     6121 aatcctccac ggccatgcac tcttccggtg ccagtcccag ctgcttgcag gcatagagat
     6181 aaatatccgg ggacggctta ccttctgcta ccatagtggc cgaaatcagt ttctcaaaat
     6241 acggccgaag tcctgccatc gccagatatt tttccgtccg ctccagatcc gtcgctgtgg
     6301 caaccgctgc ggtgatatgc ctttttttca gatcttccag aagctccacg gcaccgggct
     6361 tgcatttgat gccatcccgg tccagtgttt cttccatcat ctcttttcgt ttgtcacgca
     6421 ccacataata gtcaaaatct tcaccgtacc agtccttgag ctgtctgggg gcaaaggggc
     6481 gtcccagact gcgcatcgta agtgcctgct catctgtcat ggtataccca caggctgcca
     6541 gtgccttcgg ccagaaaata cgataatatt tttcggtatc gatcagcgta ccgtccatgt
     6601 caaataaaac tgcctgaatc attgttattc ctctgcgtca gccgtctgtc cggcatcttc
     6661 tgctgcatcc gtaacatcag taccttctgc tgggctgtcc tttgaatcac tttcgtttgt
     6721 gccgctctgg tcctcgtcag cttttccggc tttccggtca cgttttgccg ccttgcgcgt
     6781 cttccggcgt tttgccgctt ttttccctaa caatacgatc aacaggatca cgactgcacc
     6841 accgacgatg caaagtacga tcagcagtgt atgactgccc tcgacctcta ccgtgcggaa
     6901 gctgccgggc acttccatgg taaactgtct atagctgccg atcacagcgg attccacttc
     6961 cgtgtactct ccatcctgtg ctacctctat gacagtgtta tccgcatcct cacagtagac
     7021 acgcaaggtt gcttctccgg tatagtccgg tgcatcctct tccatggaat tggtcatgga
     7081 tactgtataa gtatttccct ctacctgcag atgcaatgct gcattgggat agaagttgcc
     7141 ttctgccata accagtgcct tattgttatc gtttttctct gcggaagcaa tggatgcggt
     7201 ccattcctca tactctgctt ccaacacttt attaccggta atatcactga aatcataatc
     7261 cggccataca ccgtaatagc catccttctc cggtacttcc ggaatctggt ctgccgacag
     7321 ataatccccg taaccgcatt tataggaagc cacttctaca ccatccgcct ggaaggtaat
     7381 ggtaaactgt gagaaagcgt ccggaacgtc tttgctgcag aattcctggt aggacaacgg
     7441 ggtagcaccg ccctgataac caatgccgtc tacgccaccg gctccgcctt ccacataata
     7501 gttgccatac agagttccat catcactgac ttttccggca atgctgccct ggccttcgcc
     7561 ggacatatcc agatcattgt acgcatagct gtagaaaata tcacagcctt ctccggcaat
     7621 acctccgata ttatttttac cggtgatcct gcccatgctg taacagctgc ggatcgcata
     7681 gctggcagat ccggcaattc ctcccacata gctgcctccg gtactctcta cgggtgcata
     7741 agactcacag gaaatgactg cgccatattc tgctttaccg accacaccgc cgacatagtc
     7801 ttttttaccg gtcacgtctc caaggttgcg gctgtctctg atgactgcct tgaccgtctg
     7861 ctccacatcg aaactctccg ttccggttaa agtgatatcg tcctcagggt caaaatcgta
     7921 ttccgttgcc acctgtccaa cgataccgcc cacattcgta tcggcttcca ccgtaccgcg
     7981 gttcacacat aaggtgacct ttcccttctg gaagctggaa gtatcatagc gcgcttcttc
     8041 ggttccttcc ggttctgcgg tgtcatcctc cgcatcggga tctccggggt tcacctcttc
     8101 cttgctggct gactcatccg aagtatcttc tacagaaatt ccttcatacc ggaagagatc
     8161 atcccggatt tcgctgacca gattccgcag aactctggcc tgatccatca gctcatccat
     8221 gtgggcactg gtgctgtctg tcccggcttc cagtacatcc cccaactgac ccaactgttc
     8281 aaaagccttg tcgagcctgt cattcagtgt atccagatta tctttgatac cgccactgcg
     8341 atccgtggaa gcattcgtca tgctatccag atgcttggtg gcattctctc cgaatttttt
     8401 cagtgccgat ttatagctct ccgtatcgtc cggtacggtg atggtaatgt ttccgccact
     8461 gacgtcatga tcgccatcgc caccccagtt accattccaa atgtcatttc cactgatggt
     8521 aacgtcatga ctgttgccgt tattcttgtc attatccgcc tgcttgttca gatcgtcatt
     8581 caggactttc agatcgttgc tgacgccgtt caactcttga ttgtaaatat accacagatc
     8641 atgagcggtc ccggtcagag aacttcccgc attgttggca tctttcaggt tcgaactgat
     8701 actcttggca atactggatg cctgtttgct atagctactc acatctttct gggtggcatc
     8761 caacatatcc aggaatttgt ctgtctccgt atccagttcc ttcagcttgt cactgagata
     8821 ctggatttcc aggaacggct ccatctgacc cacgataccg cccacatctt ttctgccctg
     8881 cacatatcca gtgttggtgc agttctgcag atagccctga tgcagtctgc ctacgatacc
     8941 tccggtattg tagcctacgt gctggtagcc gatggcaccg gaattggagc aataataaat
     9001 cttaccatcc gaaatacctg cgataccgcc ggtgtccgta tgagctgcca cattggaggt
     9061 actgttgatc tgctccagat tgtccatggt gatatcttcc agatcataag tcacttccgt
     9121 gctgtacgta ttgatattgc cattgttgct gcagttattc agaataccgt ggttgttgcc
     9181 cacaataccg cctgcggaat gattgccgat cacatttccg gaggattcac accgacggat
     9241 ctctccgctc tcggcattga ctccggcaat accgccgaca tccgtatcac cgaccacatt
     9301 tccggtaaat ttacaatttt caatgctgcc atagttcaca ccgacgatac cgccgacctg
     9361 atcctgactt ccagagggag atacttcccc cgtgaccgta aggttgcgga cttttgctcc
     9421 ctcctgcaca tagcggaaca atcccactgc cgaaccgtct ccggtcagtt tcacattgga
     9481 aatagtaaaa ccgctgccgt caaagattcc tgcaaacgta ggaatactga gttcacctgt
     9541 catactcaga acaatgtcgt tctgcagcac tactttttta tctctggacc aactgtccag
     9601 tacacagttc tccaccagtt tccgcagatc ctcctccgtg ctgatctcta tgactgcata
     9661 atcctgctcc tttatcgtgc tgctataacc gctgccgcct ctgtactgct cataggtcaa
     9721 agtttctgct gcataggcgg tggccggtgt ctgcaggatc agtgctgctc ccagcaggaa
     9781 acataataat ctcttactgt tcatcttctg ttccctcgct ttctgctgtt ttttctttat
     9841 ccgaagcttt cttatccgca gcttctgtat ctgctccggt tgctttctgt ccggctgtct
     9901 catcctccgg cagctcctgg gcttccagtc tgggatagtt ttcttccggg gcatcgtccc
     9961 gctcctccat cgtcacggta tccttgggac gcaccaccgc acccatctca ccatcaatga
    10021 caccgatgaa atcaccctcc aacacaccat tgacataacc cttgatgtga ccgttcatac
    10081 ggattcttcc ggcggagggc aagggctttg ccagatcccg tttcagtgta aatgctgtct
    10141 tctgaataat gatatctccg atcagcagtg tctgcggcaa tgccagcagt accaacaaaa
    10201 tagatgtcag agtaccacga ccaagagcca gtccgatggc cgctaccgct gcattggaag
    10261 atacatagct gataataaat cccgcagata ccagcatgga tccgctggta acaatggtag
    10321 ggaacgcctg gttcagtgct tcgatggcgg cttccttgat cggcatatag gtcttcagat
    10381 ccatgtaacg gctggtgata acgatagcgt agtcgatcgt tgcacccatc tggatcgcag
    10441 atactaccag atatcccagg aaatatacat tttcattcaa cagatacggc aatgagaagt
    10501 tcatccagat actaccctga atcgttacaa ccaacagtac cggcagaccg gcggactgga
    10561 aggtaaacag caggatgatc ataacaaaca atgctgtcag caccgtgatg atcgtattat
    10621 ccgttgcgaa ggaatcgccc agatccttat cactggtggc gttaccgacc agcagaatgt
    10681 catctatatt atagaacttt gccacggtat tacggatctg atccagtgct gctgtggtct
    10741 cttctccctc ataaggaaca ttcaattcca gaacaaatcg ggaattgtct tcgcccagca
    10801 gctgatcttt cgcaatacag atctgggaat aagcatccgt cagagtttct tccagatcat
    10861 cgtccagtgt gatgttgccg ctttctttct gatcataaat gaacaggaac atatcaataa
    10921 acggaacttc gtattcactg acgccactga ccagcgcacc ataatcactc tgatccagtg
    10981 catatgccga atacaacata tccgccactt caatatccag atcgatcagt tccgcaaact
    11041 gtctgggatt cagcttatcc gtcagcatat agccgtccat ggcctcgata ttgccaagtc
    11101 cctgacagga tttcaccaca tccagctttt ccagagcttt cagtgtctgt gcctcttttt
    11161 cataatcacc gttgggcaca atgactgcca gctggttcac catgccgaat tccttattta
    11221 ccatgctgac ggaaaattta ttatcactca tagtactgga ttccagagtg ttggtatcgt
    11281 acacgtagtt tgccttattg gacaggaaaa atgctataat aacacctacg atcagaatag
    11341 gcggtataat gtagcgggta gccacacaga atttaccgac cgccgtgatc ttcggcacga
    11401 agctcttgtg atgggtgttg tcgatggcct tggaaaaggt caacaacagt ccgggcatca
    11461 ggaagaatac ggctaccata ctgaaaataa tggattttgc cagaatgata cccatatcat
    11521 aaccaattct aaactgcatg aacatcatgg ctaccatacc ggagatggtg gtcagggaac
    11581 tggaggaaat ctccgggatt gccttactca aagcaacaat cacagcctct ctggcatcct
    11641 tgtcctcatg ttcttccata aaacggtggg ccagaatgat ggcatagtct accgccagac
    11701 ctaactgcag taccaccgcg atggagttcg taacaaaact gatggttccg aaccagtagt
    11761 tcgtaccttt gttcaaaata gctgccacga taaacgtggt cagataaacc gggatttccg
    11821 cataagtcgt agaggtaaat aacaatacgg ctacgataac cactgctgcc aacaacagaa
    11881 tgacgatcat atcattattc aagtcatccg cgtcacgctc ttcctgaccg atatcggaag
    11941 aatagtaaac atcataatcg gacaatgcat ccaatacctc atccagatgc tgctgtgtag
    12001 cttccgtgtc tgcttcctca tcaaaagtga cagacaataa agcctccatg tcatgataat
    12061 gatcctcggt gtcatcgaaa tccaacatac tgacaccgtc catctcctcc acctgatctg
    12121 ccagtttctg tgcctcgtca aacgtcacgt tacagatcag gatccgggcg gagccatacg
    12181 tggtaaattc cgaatccatc agatccagtc cccttctggt ctctgttgtg tccggcaaat
    12241 aagtggtcag atcgttattg accttgactc tgttcatgga taatacgcaa aatatgcata
    12301 aaacaataaa gatcagataa aatccttttc gtttgtctac aatgagcgtg gcaagtttca
    12361 tcataaagga attatctttt ttgtcttttt ctgcgttgtc tgccatagtt ccctcctgct
    12421 ttttctctct ttgtaccctt atttatgtat ttgattttat acacctctag tttaaaatgt
    12481 ttttgaacat ctgtaaagac ttactacaaa gatgagaaat tgtattaatg tcatttaatg
    12541 aacttgtgtt gattttctgc cacatgcaaa atataataat ggggaagaaa aagcaagcta
    12601 cagattgaga ttgattgttt atttttagac attttctttc tgtttgttta ttattttaga
    12661 aaaaattcag atttgtatta catataggaa atgatttctt cttttcccgg aaaggctggt
    12721 ggattgtctt atggaaaaaa tggatcgccg tgtgcgcaag acaagagctc tcttattaca
    12781 ggggctggta aaaatgttag aaacccatga tattcaggat atctccgtca aggagctgac
    12841 cgaactggtg gatatcaacc gcggaacctt ttatctgcat tatgatgata tctacgatat
    12901 gctccataaa atcgaggatg aaatgttcct ggaatttaat gaaatcatgg agcagaaacc
    12961 ggtcgttacc aattctctgg cctctcaggg gccgttgttg gaattcttcc attttctgga
    13021 ccgaaacagg gatctggcca aggtcatgat cggccctcat ggggatctgg ctttcgtcaa
    13081 tcgtctgaaa gatcagattg aaaagcgcac cctccaggtt ctggaaagtg cgcaatccga
    13141 tgctaattat gaatatctct gctccttcat catcaccggc tgtgtcggtg tcgtagagac
    13201 ctggctgaaa gaatccgatc cccagtcccc ggaagaaatg gcagagattc tcggtaccat
    13261 gcttttacgt cagcttaatt ttgctccggg gcctgcatag gctgtgtcgg ctccggcata
    13321 cctacgctca atgaagtcac tgtctcacca tccaggaaaa aggatacagt atcacctacc
    13381 tgaagttcgt ccagagtggc atcttctccg tcaatcgtaa tgatcgtatc gtcattgact
    13441 gtgatagtca tagtatctcc gctgagagtt aaggtcatct catctaacat atctttctcg
    13501 ggtttctgac cgttgggagc ttcaccatta ggggtgccgc cattgccatc aggagtctga
    13561 ccgttcggtg cttctccgtt gggtgcgcca ccgttaccat caggggcctg gccatccggt
    13621 ttctcaccgg tcggagcttc gcctgtgggt gcctcaccgc tgggagcctg accatccggc
    13681 ttttctccgc tgggtgcctc accggtagga gcttcaccat tgggtgcttc cggcttctct
    13741 gccagtgcga tggagatcac attgccatca atggcagtga tctggccgag ataagattcc
    13801 accttctctt caccgctctc cgtgctctcc gtcgtactct ccggtgcgat cgtctcttta
    13861 ttttccgatg cttccgtgct tgctgcactt gcggtggtcg atccgccaca ggctgtaaat
    13921 cctgtcatca ccatagcagc tgtcaacact gcacacatta cttttgtatt cacctggggt
    13981 aaattccatc ttctcatatt ctcattcctc ctgttccatt aaaaatctta tgaacatttc
    14041 tttcccctta tctgcctctt atgataaagg acatctgcga aggaattgtg agaaaaatgt
    14101 ggtgattctg tgaacttttc acgcatgaca catttgtcat atgtacagtg ttggcagtgt
    14161 cagatcatgt gtcgattttg ctgttttgtt gtaaatattc aaggaacgcg gtacagcctt
    14221 ccagaacgtc atcgtaggtc gttccaaaat ctcccgtata ccatggatcc gaaatatccc
    14281 gttccgatcc cgcataggaa agtagcttcg ctactttccc ctccggatct cccccgaata
    14341 tccgcagcat gttgcggata ttcgcaccat ccataccaat caaataatca tattccgcat
    14401 aatccgctct cgtcacctgc cgggcccgct tcccctcaca ggagatccca tgcctcgcca
    14461 gctccgcctc cgccggcgga tacaccggat tcccgatgcc gttccagatt tcctctgtgc
    14521 tggtggccgc ggaggcaatc tggaagcggg cggtgagtcc ggcatcggat actaattttt
    14581 tcattacgaa ttccgccatg ggggaacgac agatgttgcc gtggcagatg aaaagtattt
    14641 ttatcatctt tttatatctc catttaagtc ttgtatttct tctcaaa
//
LOCUS       DAWEJW010000071        14598 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1500_length_14598_cov_62.233927, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000071 DAWEJW010000000
VERSION     DAWEJW010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14598
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1500_length_14598_cov_62.233927"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4034 a         2980 c         4303 g         3281 t
ORIGIN      
        1 agaattgcgt gagcagcttg ctgcgaagca attcgttgac acaagtatgg cggcgacaga
       61 agcaattcgt tgacacaagt atggcggcga cagaaataaa aaacgatgac ataaacaaga
      121 gaggtagaaa aatgatcaga gtaggaatca tcggagcaac cggttatgcc ggaggagaac
      181 tggtaaggat cctgttaaac cacaaggatg ccaaaatcgt atggtacggt tcccgaagct
      241 atatagataa gaagtattat caggtatacc agaacatgtt ccagctggtg gaggatatct
      301 gcaaggatga caatctggaa gaactggcga agcaggtaga tatgatcttt accgcaaccc
      361 ctcagggatt tctggcgggc cttttgacag aggagattct ctctcaggtg aagatcgtgg
      421 atctgtctgc tgattaccgg atcaaggatg tggccaccta tgaaaaatgg tacggcatcg
      481 agcacaaatc tcctcagttc attgaggaag cagtatacgg actctgcgag atcaatcgtg
      541 acaagatcac ggagaagacc agattggtag ccaaccccgg ctgctacacc acctgttcga
      601 tcctgacggc ctatcccatg gtcaaggaag gcatgattga tgtgaacacc ctgatcgtgg
      661 atgccaagtc cggtacctcc ggtgcaggaa gaggcgccaa ggtgcccaac ctgttctgcg
      721 aagtgaacga gaacatgaaa gcctacggcg tggcaagcca cagacatacc cccgagatcg
      781 aggagcagtt aggctatgcg ggcaactgcc aggtgaccat taattttacc ccgcacctcg
      841 tacccatgaa cagaggaatt ctggcaaccg agtatgcaac actgaagaga aaaccggacg
      901 gtacacttcc tacctatgag gaagtaaaag ctgtctatga taaatattat gcaaacgaga
      961 aattcgtccg tgtgttggat aagggcagct gccccgagac caagtgggtg gaaggcagca
     1021 actacgtgga catcaatttt gtcattgatg agcgtaccgg ccgtatcatt atgatgggtg
     1081 cactggataa cctcgtaaag ggtgccgcag gacaggcggt acagaacatg aacctgctgt
     1141 tcgggcttcc tgagagcgaa ggcctggagc tggttccatg cttcccttaa agcagatacg
     1201 gaaaggatta gaaaggataa aatacataca tgaacaaaca gtttacgatc aaacccatga
     1261 ccatagaaga ttatgacggt ctgcatgccc tctggatgac cattcacggt ttcggtatcc
     1321 ggagcatcga tgattccaga gaaggtgtgg aacgttttat caaccggaat cccggcacca
     1381 gtgtggtagc catcggggag gatggagccg tagtgggtgg catcctctgc ggccatgacg
     1441 gacgacgagg ctgtctgtac catgtatgtg tcagagaaga ttaccgaaga cttggcattg
     1501 gcaaagccat ggtcgtccac tgcatgaatg ctctgaaagc ggaacatatc aacaaggtca
     1561 gcctgatcgc ttttacccag aatgacatcg gaaacgcttt ctggaaatgc attggatgga
     1621 caaagcggga agacttgaac tactacgact ttactttgaa tgaagagaac attacggcgt
     1681 ttaataaata gatgccagga ttgtggaagt gcgtagcacg aagcaatccg taggcatcaa
     1741 agtcgggggc ttttgaccca gacatcataa ataaaacgga agcgaaagga gattattcca
     1801 acatgaaaca gatccagggc ggtgtgaccg cagcaaaagg ctttgaggca gcaggtgtgg
     1861 aggctgccat taaatatcag aatcgtaagg atatggcact gatcgtaagt aaggcacctt
     1921 gtaccatagc cggtactttt acctccaatg tggtaaaggc ggctcctgta ctgtgggata
     1981 agcagatcgt ggaacattct gcatttgcac aggcagtggt agtcaattcg ggaatcgcca
     2041 atgcctgcac cggcaaacag ggactggact gctgtgaagc agaagcaaaa tgtgccggag
     2101 aactgttggg cgtgcccacc gatgccgtac tggttgcatc caccggtgtc atcggtatgc
     2161 agattcccgt agacaagatc accgctggca tcgagaaact ggtggcagcc aaggcagata
     2221 ctctggaggc tggctccgat gcagcccatg ccattatgac caccgatacc atttccaaag
     2281 agatcgccat cgagacacag atcggtggca agactgtgac tctgggcggt atgtgcaagg
     2341 gttccggtat gatccatccg aatatgtgca cgatgttagg ttttgtaacc acagatgtga
     2401 aaatttccaa ggagatgctg cagaagtgcg tcagcgcaga cgtagtggat tccttcaaca
     2461 tgatctccgt ggatggagat acttccacca atgatacact gctggtgctg gccaacggca
     2521 tggctggcaa cgaggagatt accgcagagg gcgaggatta cgacaacttc tgcaaggcac
     2581 tgcatgagat caccacattc ctggcaaaga aaatggcagg agatggcgaa ggcgccacag
     2641 cactgttcga gaccaaagta ataaacgcag tcagcaagga agatgccaga actctggcaa
     2701 aatccgtgat ctgttcttct ctgaccaagg cagctatctt cggacatgat gccaactggg
     2761 gacgtatcct ctgtgcactg ggttattccg gagccaaatt cgacccggaa aatgtggatc
     2821 tgtattttga gagcaagagc ggcaagattc atatcttcgg taatggtgtg gcatgtgatt
     2881 acagcgagga agaggccacg aagatcctct ccgatccgga agtcaccgca ctggtggata
     2941 tgcatatggg tgaggcagaa gctaccgcat ggggctgtga tttaagctat gattatgtga
     3001 agatcaatgc ggactatcgt tcctaaaaaa gaacgaccgg aaagataaat gacaggcgga
     3061 tcacggtatc tgcggaaatg aggataaaaa tggaaaaaga tatggaacag gtaatgaaaa
     3121 aagcagaggt gctgatcgag gcgcttcctt acatacagaa attcaaccgt aagatcatcg
     3181 tggtaaaata cggcggcagc gccatgagca atgaggaact gcagaaaaac gtcatcaagg
     3241 acgtaacgct gttgaaactg gtaggtttca agcccatcat cgttcacggc ggcggtaagg
     3301 agatcagccg ctgggtcggc aaggtcggaa aagaggcaaa gttcgtcaac ggtctgcgtg
     3361 tcaccgatga ggagaccatg gagatcgcgg aaatggtact gaacaaggta aataagagcc
     3421 ttgtgaccat ggtggaagag ctgggcgtga aagcagtagg tgtcagcggt aaagacggcg
     3481 gcctgcttca ggtagagaaa aaatattccg acggtcagga catcgggtat gtgggtgaga
     3541 tcaccggcgt gaatccggat attctctttg acatgctgga gaaggattac ctgcccatca
     3601 tcgctcccat tggactggac aaggattatc acacctacaa tatcaatgcg gatgatgcgg
     3661 cctgcgctat cgcaaaagca gtacatgcgg agaaactggc atttctgacg gatatcgagg
     3721 gattgtacaa ggacatcaat gacaagtcca catttatttc cagattgaca gcttccgagg
     3781 cagagggact gatccacgac ggttacatcg gcggtggtat gctgccgaag ctgaacaact
     3841 gtacttctgc gatccgcaac ggtgtaaacc gtgtgcatat tctggatgga cgtctggcac
     3901 actgcctgtt gttggagatc ttcaccaatc agggtatcgg aactgcgatc gtatccgatg
     3961 aggaggaaaa ataaaatgag cgacactatg aaagaatata ttgcagaggc ggaaaaagac
     4021 ctgctgcata cctataaccg ttatcagatc gttctggata agggcgaagg cgtacatctg
     4081 tatgacaccg atgggaaaaa atatctggat ttcgtagcgg gaatcgctgt atttgccctg
     4141 ggctatcaga acaaggcata caacgatgcg ctgaaagcac agatcgacaa ggtgatccac
     4201 acctccaatt attattacaa cgtgcctgcc atcgaggcgg ctcgtaagat caaaaaagta
     4261 tccggcatgg atcgtgtctt tttcaccaat tccggtgcgg aagcagtgga aggtgccatt
     4321 aaggctgcca gaaaatatgc gtatctgaag gatggctgta ccgaccatga gatcattgcc
     4381 atgaatcact ccttccacgg cagaaccatg ggagctttgt cagtaacggg caatcccaag
     4441 tatcgtgagg cgttccagcc catgatcgga catatcaaat ttgcagaact gaatgatttc
     4501 cagagtgtgc tggatcaggt gacagacaag acctgtgcca tcattatgga gactgtacag
     4561 ggcgagggcg gactgcgtcc cgctaatgaa gaattcttaa aacaggttcg tgatctgtgc
     4621 gatgagcggg atatcctgct gattctggat gagatccagt gcggcatggg acgtaccggt
     4681 tacatgtatg catggcagcg tttcggcgtg aagccggaca tcatgacttc tgccaaggca
     4741 ctgggctgcg gtgtaccggt aggtgcattt atgatgacgg agaaagtagc acagcattct
     4801 ctgacagcgg gagatcacgg aaccacctac ggcggtaacc ccttagcctg tgcagcagtg
     4861 gaaaaagtgc ttgatttatt cgaagaggac catataatag aacatgtgag agaggtggct
     4921 ccctatctgg aacagcgtct gaacgaactg gcagacaagt atgactgtat caaagaacgc
     4981 cgtggcgtgg gactgatgca gggactggta tttgaccatc cggtaggaga cattattaac
     5041 agagcattgg agaagggatt aattctgatc aatgccggtg cggacattat ccgcttcgta
     5101 ccgcctctcg tgattacccg ggaaaatgtg gacgaaatga ttgctatttt ggacacctgt
     5161 atcgaataag aggtttaggt gaatttatgt ttttgaaaaa aagaattatc gctacagcag
     5221 ctgtgatagc catggctgct gcagctgttt acggaagtac gcaggagatg agtcagacac
     5281 aggagagcgg aagcggactg cagctgtttc gaggaaagga gacaatctat tgctggtatt
     5341 ccgatgaggc attgtccggt tatatcaatg cggcagcagt atccttcgga gaacagaaca
     5401 atggagtccg tgtgattccc gtacttactt ctgacagtga atatctggaa gctatcaatc
     5461 aggagaccct gcattccgat cagataccgg atatctacct gttgagcagt gactctctgg
     5521 aaaaggctta tctggcagga ctggcatcga aagtgcctga cactgtggga atctgcaata
     5581 cagaccattt ttcccaggcg gctctgtctg cggtaaccta tgatcagaag ctgatcggtt
     5641 acccggttta ttttgatacc agtgcgttgg tatataatga agattatctt cgaacctggg
     5701 caacccagca ggcggagaaa gaactggcgg gaagcagtga gaatgatgaa cccatagggg
     5761 aaggagaaga gatcatcgag gaagattctc ttccggaaga ccagaccacg gaacaggtga
     5821 cagcggatga ggcagcggta aatgcactgg cagagcagta tttcgcggaa gcacttccct
     5881 ccacagtgga tgatctgctg aatattgcag atacctttga tgcaccggaa ggtgtggaag
     5941 gtgtcatgaa atgggacgtg aacaatattt tctacaacta ctggatcgta ggcaactaca
     6001 tgatcgtagg cggtgatccc ggggatgaca gaaatgatat tgatatcaat aatccggaga
     6061 ccattcagtg tctggaagtg tacaaggcac tgaaccagtt cttctttatt gaatccgata
     6121 cggtgactta tgattctgtg atccaggact ttatcgatgg caagaccatg ttcaccatcg
     6181 gaaccacgga tgtggtcaaa cggctggaag aggcaaaagc ggacggcagc cttgcattta
     6241 attacggaat tgcccgtatg ccggatgtca gcgtcgaact gcagagcaga tctttgtctg
     6301 ttacgggagc ggcagtcatc aacggctatt cggaacacaa ggaactggcg gactgcttcg
     6361 cagcgtatct ggcagaagga tatgcggacg gcctgtatga acgctccggc aaaatgccgg
     6421 caagtcttca ggcggcagag aatgcagaca acggtgcgct tatgatcttt gccgatgagt
     6481 atgcggacag tgtgtcattg ccgaagatgc tagagacggg aaatttctgg atgcagctgg
     6541 aagtactgtt ttccaaggta tggaacggtg acgacgtgac tactctggta cagcagctgg
     6601 acgagaccat agccggacag gtaggtggta cagagaacta aaaccgttac aataaaatat
     6661 gaatgaacga accggtcaca ggatagacta ctactaaaaa gaaaaggtgg tagaattcta
     6721 tgatcggttt tttgattgct ttagtttccg gtgcgctgat gagcatccag ggagtgttca
     6781 acacccaggt gacgaaatcc accggcatct ggaccgccag tgcatttgtg cagtttacag
     6841 cattgctggt atgtctgggg gcatggctgt gtacggagcg gacttcattc ttgaagatag
     6901 cgcaggtgga gcccagatat atgcttttgg gaggggccat cggagccttt atcacctaca
     6961 ctgtgatccg cagtatggat gccctgggac cggccaaagc ggtaatgctt attgtggtcg
     7021 ctcagcttgc tgtagcatat attatcgaac tgttcggatg gttcggtgtg gagaagcagc
     7081 cctgggagtg gcggaaagcc atcggcatgg ccgtggcgat tgtgggtatt gtgattttca
     7141 agtggaaatg atccagagct atccgcgaag cggcaatttt acgaaggggt tctgaatcgt
     7201 taccacaaat gcgaaaacgg cttgtaaatt ccgtgcgatt ccagtacaat gaaagttaac
     7261 agcatagaga ggcaatcccc tgaaattttg tgaagaaatc aggaaaatgg aaaggggact
     7321 atgatgtttt cgataaggtt ttttaaaaca aaatatgcag gtttgatcgg aatgtttttc
     7381 agtctggtga tgttcatgac cggctgtcag aggaaggatg aggccattgt gctgattcca
     7441 tccggagaag atgtggcgga atttccctca ctggaagcgg tggaacaggc agcggagcct
     7501 gcaggcagca gcgtgacagt gcaggaaccg gcaaaggctg aaccgcagat gatatatgtc
     7561 tatgtctgtg gcgcagtgcg ggatcccgga gtcgtggaag tgccggaggg cagcagagcg
     7621 gaggaagcct tgcgactggt cggaggtatg acagcggagg cggatccttt ttatgtgaat
     7681 ctggcagaaa cggtgacgga tggtcagaag ctgtattttc caacgttgga ggaagcggag
     7741 actctggaag ccgaggtaaa agcgacggcg gagggacttg tgaatatcaa cacggcatct
     7801 gcggaggaat tgtgtgcgct gccggggatc ggagcttccc gggcggcaga tattgtgcgc
     7861 tatcgggagc aaaacggagc ctttcagacg aaggaagaca ttatgaaggt atccgggatc
     7921 aaacagaacg cgtatgacaa attgtgtgat aagatcacgg tacaatgagg gcttatgccg
     7981 ggaccgtgaa gagaaaagta agggatagag aggttaagga aagatggcag gcaagaaagt
     8041 gctggtagtg gatgatgaga agctgatcgt gaagggaatc cgtttcagtc tggaacagga
     8101 caacatggaa gtggattgtg cttatgacgg tgaggaagca ttgcagatgg cgcataacaa
     8161 ggaatatgac atcatattac tggacgtcat gctgccgaag ctgaccggct ttgaggtctg
     8221 ccagcagatc cgcgaatttt ccaatgtacc ggtgatcatg ttgaccgcca agggtgatga
     8281 catggataag atcctgggac tggaatacgg cgcggacgat tacataacca agccttttaa
     8341 cattctggaa gtgaaagccc ggatcaaagc tatcttccgc agaatggagc cgaaaaaagg
     8401 aaccacagag aacaccggta ttctggaaag cggcgatatg aagctggatc gtgacggcaa
     8461 aagagcctac atccagggca gggagatcgg actgacggcc aaggagttcg aagtgctgga
     8521 acttctgatg ctgaatcccg acaaggtata cagcagggag aatttgttga agctggtatg
     8581 gggtacggat tatcccggtg atgtgcgtac agtggatgta catatccgga gactgcgtga
     8641 gaagatcgag tccaatccca gcgagcccag atacgtgcat accaaatggg gtgtcgggta
     8701 ctactttaat aataagtgag actatgagaa ccggcaaaga taagaggaaa tgattcgcca
     8761 tgcttgcatg taagaatcaa tttctcttat ctttgaaggt ggctcgaata gagccaccgc
     8821 cacgcaaatg aacaaaagta gctgcgaagc agcggagagc acgatttatc gtgcggtttt
     8881 gtgaatggcg tggagaggtt cggaactgag gtttttatac tgtgaaaaaa tggaataaag
     8941 aacgttttgc aactatatgg gaaaagctgg agagcatgat cgcgcttcga agtctgcggg
     9001 cgaggatctt cctgctgacg ctggtgatcg gcctggtgcc ttgtattgcc atgcgccacg
     9061 gtatcgtgag caattacgag gatctggcgg tggaacagcg gaccacagtg gtacagaacc
     9121 agttgatgat cctggccaat catctgatca gcaacaatta tttaagcagc catggtgtca
     9181 gagaggacac cggcaattcc agagaagtca tcaatgcgga actggagatg ctgtccaatc
     9241 tgtatgaagg ccgcgttatg atcatcaaca agaatttcaa ggtggaaaaa gatacctacg
     9301 gcatcagtga aggaaagacc atcatttccg aggaggtcat caaatgtttt cagggggaaa
     9361 acatttccca ttatgacccg gagcacggtt acatagaaat gaccactccc atcatggata
     9421 ccacggcaaa cactacggac gaaaacggaa aaaggaatcc ggatgccatc cggggcgtta
     9481 tgctgaccag catttccaat gacagtatcg taaatgccaa ggaagtgctg aatcgtaagg
     9541 cgtacattct ggaagtgatc atgatggtgg caatcctggc actggctatc atactgtcca
     9601 gagctttgac gagacctttc aacagagtca cacaggccat caatgcggta aaggaaggct
     9661 tcagtgatga gacgatctct gtgccagatt atgtggagac cgtacatatc gtagatgcgt
     9721 tcaaccagct gttgaagcgg atgaaggcat tggacgattc ccgacaggaa tttgtggcaa
     9781 atgtgtccca cgagttgaaa acacccatga catccatcaa ggtattgtcg gattccctgc
     9841 tagcacagga aaatgtaccg gcggagctgt atcgagaatt catggaagac attgcttccg
     9901 agattgacag agagaaccag atcattacgg atctgttggc actggtaaaa atggataaaa
     9961 aggtacagga tctgaatatt gtatcgttga acatcaatga tctgacggaa ctgatcttaa
    10021 agagactgcg gcccatcgcc cggaagaagg atgtggaagt ggtatttgaa agcgtccgtc
    10081 ctgtggtggc agaggtggat gaagttaaga tgaccctgat catgacgaat ctggtggaga
    10141 atgccatcaa atacaataag gatcatggct gggtgaaggt gatactggat gcggatcatc
    10201 agtattttac ctttgaagtc agtgattccg gaatcggtat cccggaggag gctctggcac
    10261 atatctgcga gagattctat cgtgtggaca agtctcattc cagagagatc ggcggaacgg
    10321 gactgggact ggcgatcacc aagagcgccg tcctgatgca caagggatcc cttacggtga
    10381 cgagtaccga ggggcagggc tcctgtttcc tggtgaagat cccgctgaca tatatcgcct
    10441 cttagacgga gctgttgtaa gaaagattgt gtagttatga agagagttaa aaaatatgtt
    10501 ttgaccggta tgcttctgat gttgctggca gtcctgaccg gctgtggcac gagagagccg
    10561 gagaccggca aaaagatatc ggtgtattat gtaaataccg cagagacgaa ggttgaggtt
    10621 catgaccagt atctgaacac caagacaccg gaggaacagt tggaagagac catgacattt
    10681 ctctccacca ccccggaaaa gctggaatat aaggcgcctt ttgacatggg atttcaggtt
    10741 ctggattatg atgtggaaga cggaaaactt gtgatcaatg tggataaggc atattccgaa
    10801 ctttcggtca ccacagaagt actggtgcgg gcggccgttg taaggacact gacgcagctg
    10861 tccaatgtga aatatgtgac gattaccttg gaaggcagtc agttatatga caatgcaggg
    10921 gaattggtag gctggatgaa tgccgagcag ttcatcaata atgacggtaa cgagatcaac
    10981 acctatgaac tggtgaaggt aaagctgtat tttgccaatg cagacggtga taagctgatc
    11041 gcggcctaca gagaaaagca ttattccacc aatactccgt tggagcgatt cgtggtggag
    11101 gaactgatcg cgggacccag cggacagatt gagggtctgt acccggtgat caatccggag
    11161 accaagatca tcaatatcct gaccaaagat ggaatctgct atgtgaatct ggattccagc
    11221 ttcctgaccg tggtgaacaa tgtttccacg gaggtagcag tttactccat cgtgaattcc
    11281 ctggtggaac tggataatat caataaagta cagatcctgg tcaacggcga agtgccatct
    11341 accttcagca attccacttt tgaacggaat ctggattatg taacgacttt ggaacagtaa
    11401 attttgaatt tatgcgggac aggactgttt ttgcgttgga ctgtcggaac agtcctgact
    11461 gtgggcaggc gggattgcct gcggaaggga gcttaatgag acgaccgctt tttatggcgt
    11521 gtctgtgtct cgtgatagtg ctcgccatag gaagaatact gacgggggca gacaccgggg
    11581 atgccggcgt actgccgcct gacggaagtc cggtgaggat cacgggacgg atagacacaa
    11641 gaacttctga aacgattatt ctgaaatcaa tttctattat tcaaaacgat ctaaaatatt
    11701 cctattctgg aaaattacaa tgtgagttga caaacacaca ggaggtacag agcctgagac
    11761 tgggccagca tattgttctg gaaggcgttt tttcgcactt cgatgctgcg accaatcacg
    11821 gggaatttga cgtgcgggcc tattctgcgg gaaagggcat cggtggcaga gtgcggaaag
    11881 cacaggttct ggcagcggag gaggactata gctttttgag agaaaagttg tttgcctttc
    11941 gaaggagatt acacgacaga ctggcaaagg tattcccgga gaaagaggct tccgttatgc
    12001 agacattact gctgggggag aaggaagaaa tggatgcgga ggtaaaggcg ctgtaccaga
    12061 ggaatgggat cgcccatatc ttaagcattt cagggctgca tattactttg ctggggatgg
    12121 gatgctaccg gctgcttaag agactggggg ctccggtcag agtggcagca gcgggaggtg
    12181 ccatgacact gcttctgtac ggtatgatgg tgggcatgag tgtatccgcc agccgtgcca
    12241 taggaatgta tctgctgcag atgttgggga tattcgtggg aaggacttac gatatgctga
    12301 caggtgtggg gctgttggcg gcattgctgg tgttgcagca accggagcgg cttggggatg
    12361 tgagcttttt gatgtctttt ggggcggtgc tggggatctg cttgttgaca ccggtgtttg
    12421 ccggggatgg tagggaagat aatgtcggtg tggaaacggc cagtggcgtg gtggcagggc
    12481 ttgaagagaa aaagagggcc gacgggaagc gcggacttgg tgaggcatta ggctttgcag
    12541 agaaaaagag gactgatggg aagagcggat ttggtgagag gtctgacttt gacacggaac
    12601 agaggagact gggaaacggg gggaacggtt ttagagtacc ggcatggctt ctgaccgtga
    12661 cagatattct gggtgattcc gcttacgaga ggaacaagta ccgggaaggc tggcggaagg
    12721 tggtgtatga aaggctttgg cgcatggtgt cagcacttaa aagcggtttt gccgccagtg
    12781 ccggagttat tctttttacg ctgcccatac agctctggtt cttttatgag atccctgtct
    12841 actcggtatt attgaacctg ctggtgttgc cctttatgag tgtggtgatg gcagggggaa
    12901 tcctgtcatt gatccctgga ctgggaattg cagggacgct ggattgtctg atcctgtggt
    12961 ggtatgaatg gatctgcgaa cggttcggag aactgccggg agcggtatgg tgcgtgggcc
    13021 gcccgacaaa gtggcagatg gtcgtttatt atagtgggct atttgtgttg atcattggca
    13081 ggaattatgc ggaaaaatgg aagcggcaaa gactttatgc agcttatgtg gcggaaaata
    13141 atcatggaga cggacaccgc actgaaagag aaaggcagcg tcgggaaaca cgcagagtgg
    13201 atgatagcgg taggggtaga aaaagagaga gtaacagaaa aaagatgcag cattctgaca
    13261 ggtactctga aatatgtccc accagatggc ggcatgtttt agcaaatttt tggtatacat
    13321 ggcagggagt gatgacatat aggaacggag ttatgtgccg aattgtagcc gcaatgattt
    13381 tagtattaat agttggttta ctgaccggca attttgatcg gggaagccgt gtgacttttc
    13441 tggacgtggg acagggagac ggtatcgtgg tggagaccgg gcagggggca tatctttttg
    13501 actgcggcag taccagtcgg aggaaaatcg gtgaatatgt gttgaagcct tatctcaaga
    13561 gccggggaat ccagtctcta cgcggtgtgt ttgtatcgca cccagatgaa gaccatatga
    13621 atggcatatt ggaactgctg gagaacggtg gagaatgggg aattaccgtg gagcagatgt
    13681 ttctgcctgc gattacggaa gcggagcgca gggaggcttt tgaaaaattg cttgttgccg
    13741 cggaatatgc cggagtaccg gtaagttaca taaaatgtgg cgatgagatc agggatagtc
    13801 gactctggct gcggtgtctg cacccggagg agaatacgac tcttgcggat gccaatgcct
    13861 attcggaatg cttctacgta gaggtatttg cgaaagcagt gaagtggggc gcggcagagg
    13921 ggatggaagc aagtggagag ggcggaagag ctgctagcga agtttacggt gagaacggca
    13981 gctttgctgc cggagttact ggtgagagaa ccggtcatgg cgataccggt gaaaggaaaa
    14041 acttcggcgt gggtgccggg aagctaagca ttctcctgac aggggatgtg gaaggcgaag
    14101 gggagcagca gctgacacag gaattgcaga cgttgaagac gttacaggaa gcgaagacgt
    14161 tacgggtagc gcaggaatca caggctttgc agaatgcgca gaaattgcag gaatcgcagg
    14221 aaccgcgaga acagcaggag ttgtgggaat cacggagaca aggtggcttc aaggtagaca
    14281 tcctgaaggt agcgcatcac ggctcaggct attccaccag ttctgagttc cttgccgccg
    14341 ccggccccgc tgccgccatt atctcctgtg gtcgtaacaa ttcctacggc catccccatg
    14401 ccgcgacgct acagcgcctg gaagatgcta aggttccctg gtacctcacc actgactatg
    14461 gagccctgac agtaaccgtc gacagtcacg gaaaccggtt gcaagggtat ctgagaagaa
    14521 aataagccgt tggggttcta ggtagttggg gcgtgagagg aagaaaagta agaataagcc
    14581 ccggggaagt caagaagc
//
LOCUS       DAWEJW010000072        14339 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1533_length_14339_cov_36.638687, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000072 DAWEJW010000000
VERSION     DAWEJW010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14339)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14339)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14339
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1533_length_14339_cov_36.638687"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3788 a         3189 c         3292 g         4070 t
ORIGIN      
        1 ggtggcactc attttttgca aaccggttat ttgttactta acaggaatgc tccttatgct
       61 gcaaacttct gctcaatgca ggtatttaag atttcttccc ggcactcagg atccgcagaa
      121 gcagatgcaa gaatcgtgta ttttgcagat tccaatgcgt ttccgctcac catataggac
      181 tgtacccagg agatcagttc cctgacaccg cagcttccgt ctgtaatcat agattctttg
      241 cagtgttcat ttacatccat gacagtcgat gccattaaag ttaccgtgga tctgtcggtg
      301 cagccggtaa tgttcatggc acgttccgta aggatggata cactgggcgc atccatgtca
      361 aagaccaggt tcattctgga aattaccgac tggttcatgt cacggcatcc ggcatagttg
      421 ttatttgtgg taatgatcac aaccgtatcg ggatgtctgt gaatgacatc accggtcgta
      481 agagtaatac tggcacagcg gtcaagcagt gcatttagac ctaccaggac tcccggatta
      541 gaaattacgg taggctcctg gatctcgatg acatagccat ttttcatggc ttctacgagg
      601 ggagtctcca cgtaacgaaa gcgctgtccg gtggatgcgc ctttagattc tgccctggca
      661 tccgtctcca taacttctaa gagcttctga tagacttcgt cttcggaaac gtcatcctta
      721 taaatccccg tgagcttttc ataagcggag gagggatcca tctgtaaatc caggaaggtc
      781 ggatattctt ttacaccggt gtgatctttt tcattttccg gaagcatctg cccaagaaaa
      841 tcatagattt cagtgtttgc agagcaggta taggacagat aaggaagacc cagtcctgca
      901 gcaatcgctt tggcagcctc tgttttaccg gttcccgcag gcccgcgcat cataaaatta
      961 cgcatgggct gcgcagaaga cgtggttgct tttgcgtggg cgcagatggt aactacttcc
     1021 gggggaatga cataccagtc ctccagtgtg ggaattaaca gctgttcggt agcggaaaaa
     1081 tctctttcgg aaaacttata tttcccgatc agatctttct tgtctgtgac tacggaagct
     1141 ttcactctgg gagttgctgt taagatttca aactcaccaa aaagtacact ggtaggagag
     1201 tagttaccgt tctttagatt catttctgta agaaggggga tattaccggt acgtgaatcg
     1261 gcaatcttaa ttccggtgtc tccgagattt ttggcatttt caatacgacg gtagaaatta
     1321 tcacagagta taaatgctgc tttttctgcg gttgccatat cggaaaatcc ggcttcctta
     1381 cattttagaa gagtatcata attttcctta aattcatcgt ccttcatagc ttccggaatc
     1441 agggtaaaaa agagggcact gccggtacag cctttttccg ataacatata taccgttgcg
     1501 ttgctgtttg catccgcaaa gcagccagcc cgaaaggatc cgctcttttt attaaataca
     1561 acgacatgga tctgcccggt tctgctgtgg tattctgcca tgatctgctc attttgtgta
     1621 ccaatcgcac tgtttgacat agaagaggag atggtcgtgt ccatttccat gtattccatc
     1681 agtgcctgta aaacaggacc gtggtaggta gccactgtgg tagttgtatt gtactttgat
     1741 ttgtcggtac tgctttttgt gtgcgcagcc ttatcaaagg gttccggcag accaatattc
     1801 caggtccatt tttcgtataa gcgtgtgtta gccattgttt ttcatcctcc ttatatccag
     1861 gatacctgta tggtattccc ggcaatcttt gcctctaatt cattgtcacc aagtacacaa
     1921 atcagttcct gccagtagtt cttccctgga aatgcatcca aagagcacag gaaacgttcc
     1981 tttttctggc agttgattac aatgtcacca ttttccttga tcgtaagttt gttatgcccc
     2041 tgtgcatgaa gctctgtgat ctgtttgtcg agaatctgtc ggcctaccag atcaaaccat
     2101 gcctttacat caaccaccgc aggctctttt ggaatggatg cctcgcagtc ttccgcattc
     2161 tccggaagga agtttccgat ggtcatgggt tcaatatgga tccgcccata aaaatcaaaa
     2221 gaaacatccg catgggtaaa agccgccatg ttttccacct ggatccgtac cgtgtcaccg
     2281 tccatgagat tacgcaaggt aggttctttg tcccatttcc agacagcatc cggataggca
     2341 gattttaatt tctcagagat ccgaagcctg tactgtgtca taagaacctg cacatcctct
     2401 aaagagagtg tcatgccgct ctccctgttt ggggatagga tctgaatact ttcattgctt
     2461 tgttgcagct tccgctttcg atgcagtgat ttgctgcatt tcctatttgt gcatctttct
     2521 gccaaaaaat aatggatcag caaaaagagt acccaaagac caaacagaat taaaataaca
     2581 atctgtccgg gtttctttgg tactaacaat gcaatcgcaa ggattacggg cagcaaaata
     2641 cggtagcttg tggtaaatac attctttttc attggattca tcctttctga ttttgaaaat
     2701 atggaaataa aaaaagagga atcacagtcg tgaaataatc tcacgattgc aattcctcac
     2761 ttttgtccag tgcattaggc accaaacggt aatccttcct caccacagtc atcggcaccg
     2821 gaaaaacctg atgcatccgg ttcgggtgtg ttctgcggcg tgtttttggg tttactggtg
     2881 tgggcatacc gccagtcata cagtgtgact ttggcacgcc acccctcact tccgtcattt
     2941 cttttaaagg tctcaagttc aagatccccg gtaatggtga taagacttcc tttttttacg
     3001 ccggcatctg tcagccgttg tgctgctttc ccgtagagga cacagctaaa aaagttgggc
     3061 ctttcatgtt ccccatagcc tttgtttacg gcaagaccaa actgcacgta tttggtattt
     3121 ttcccattcg tgctggtctt aagctccaca tctgcggttg ctctgccggt agtagtgata
     3181 gttacaagat ctcccattct ttcttctcct ttctgggtgt caaaaatttt tgtaataaaa
     3241 aaagtgccaa aaaaatgcca cttttaaaga tggcaattct ttgacactta ttacaaactt
     3301 aaacaacgtg tgcttctatc ccgaacggaa tgcacaaaaa tcaattacaa aataagagta
     3361 gcatagatta cagaaactgt caaatattat aaaaatgatg attcagaggt tgttatccta
     3421 taatcgatga aacttattcg cttacggagt tgctttttcg cttcggatac actgacagta
     3481 gaagcaccgt ttaagcgctc ctgctcggcc cgtaatacat ttgcacgcag atgcagcatc
     3541 tgctcttctt caaaggcatc aatactcatc agagaaagag ctcctttcaa attttgtaat
     3601 ataaataaat tctttggttt cttctgttaa agtggaaata gaattatagt caattcatag
     3661 tgttgtagta ttagaaaaga aattctccat aattttggcg atcatcaatt atgtaatcaa
     3721 ttttaacgga tttctcattt tcttttacct ggaatacaaa ccataaatga ggccaaaagt
     3781 ttgttacacg atatttagct ggaatatagc gtaaccgagc acaaccaata aagggtgtca
     3841 tttcaagcag ggatatagct ttatcgtagg aatccagcaa ccgcagagca cattcttcac
     3901 cattatcgcg ggcgcaccgg taggtatact cttcaagaac agtattggct gctggagaaa
     3961 tatcaacagt gtacatagta ttatcctctt aacgcagctc tgagtttggc agaagcactt
     4021 ctggctggaa tatcagcaat tccggatttt ctaagttctt ccacttcgat cagcttttcg
     4081 cgtaattcta attgtttttc tctctggtca aaagcatgga tatccattac tacaagatca
     4141 ccctcaccgt ttttggtaag aaaaaccggt tcgccggtag ttttgcataa gttggcaatt
     4201 tcggtataat tctgcctgat tgtagcagaa ggcttaatga tagtctgcat atctgtactc
     4261 ctttcggcat agtaatattg taacgtaatt ttaattgaat tatactatat gttatggata
     4321 ttcgcaatgc atatataaaa atcgctaata aaacctatat tagttgaagc acaatctgct
     4381 gcttcagata caggtttgca atggcagaca gcacaggaat ccgggaagag cttgttcttg
     4441 cacttgtcac cagggtgtta tagtctgtct cctcatcgtt atagagtgct tccatgaggg
     4501 cagtggaatc cttcagatga gaaatgtgat tttcattgca caggataact tctgcagcag
     4561 acagatcctg gttttttaat agctgcatga cctgtgattc taaaggatcc aaccggacag
     4621 aatggaaaat acacaaagca gttttaagcg gcaccttgcg ggataagagg agtttcacaa
     4681 aagtaagcag cttgacagga atgctgtttg gcaccgcctg aagctgcagg tggccgatca
     4741 gagcatagag tgcatccacg cccacataat cttccccgca gactaaaagc tttcgctgta
     4801 aaagtctgtt tagatactgg tcgaactctt cctcactaag gacatggagt tctctttctt
     4861 tttcataaaa ctcctttcgc agttcatcat aggttaagat tcggaaagaa agacaggacc
     4921 ataatacaag ttccatggaa gtcagaccat attccctgtc tgcacagaca atggtgggaa
     4981 gtccatgttt attgaatttg aaggttccaa cagcagtgta aagtctaatc atagtttttg
     5041 tcactcctct aaagtttttt atgctgataa aaaatagtag atccgcagtt tctgctttgc
     5101 tctaaagatc ccggtggaaa cgacactgtg gcttacctta tagtaagcgg caagctcaga
     5161 gatgttgtac ccacagaggc ggtttaacag gaacatttcc aggtgcttcc tggcaggttc
     5221 tgtctggtta ttttcataag cctttaacat gagccgggtt tccaaatcgg aaaatgcctg
     5281 atcgtttgga tccttcagca catcggaaag tgtgagctct ttgtcatcag atagcgggga
     5341 gtccaaagaa acggtgattc cctgccattt ggctgccttt ctacactggg aaagcagtgc
     5401 atgtcggatt acaacggatg caaacgtagg aaaggaagcc cctttatcct gcttgtactg
     5461 gatagctgca tggcagagtg cctcacagcc ggtctggtaa aggtcattgt attctagctc
     5521 ctccgtattt ttgttcatgc ctatggtgtt aagaataatg ttcctgataa gatataagtg
     5581 tccctccact aactgttttt ctttttcggt catcatgtcc attcacctcc ccttatgcga
     5641 ctttcacatg gtggtttgta agtacttttc tgcattcctg catcaggagg tcacagcggt
     5701 attctgaaaa ctgtccgatg tgtgcctgag cgtctgtaag acagaagata tcctggatcc
     5761 agcggtaggc atcttttctg gagaaaatcc cgtttttcca gatggcatca aacagacgat
     5821 gcgtttcaat tcgtttgtgt cgaaggtcac cgttagctaa agatcctttt ggttccaaag
     5881 tacctgcatg aacaccaaca taagagtcac atgcaggata cctggagcag acatacaaat
     5941 actcatccag ggcacgctcc ttgtagacgt aagaggcttt tcttaatacg gcagttttgc
     6001 cacagtaagg gcatttcatg ttcttgggtt tcattttggt tttcaaggtg atcatctcct
     6061 ttctacgtag ggaatacgta ataaatcgtg tttataagta tattttagaa aagggtgcag
     6121 gcagtgccta cacccatgga gcagtttctc ctgcaaagat ctgccagact ggaaataaat
     6181 ggaaattatc agaagagatt tgtgatttcc gtagtgattc tggggatcac tgtgttgtta
     6241 aagatgttgg tgagtgcggc aagaagcagc gcacccagta cgacggcaat gatgatcttg
     6301 acaccatcgg aaatgtagaa atcgccgcgc tcattgctaa gagcgttgtg gatacgggtc
     6361 ttcatggata aaatttttct gttcattgga ttctccttat gcttgatagg tgtacgggtt
     6421 acgaaaattt ttacatgggg tcacctccct tacgcctttt gtcattaaaa aaaggccccg
     6481 agggacccga tgacttctat ggacaggacg accacataaa taagcaggat gcagaatagc
     6541 atcatcatgg aatatttctg catctgcttg gggcgtttag cggcttcttt tttcaaatta
     6601 ttctgctcaa tctgccgcat atcgaaagag agcatcttaa agtacatctg ttgatcgtct
     6661 ccacgcagtg tgccgatcag cccccggata atatccgata acatggtaga ccccacacga
     6721 ttttgcaggt gaagcagggc attttcataa ttgccggtac gcatatcagc aatcgtggta
     6781 tccagttctg cacccaggtt tggaccggca atccgtctgt aagagacaag gagctttaga
     6841 acatccctgt cggtggcaat actctgcccg atactgatgc agaacctggg aagctcggct
     6901 tcgataagtt tctttcgttt tttaacgagg tcgaaagcct tgtagtaggt agaaaaccac
     6961 attagaaccg ctagtcccag tatcaggaat ccaaaaagcg gtgaggataa aaaaagcggc
     7021 agcgataaag ctgccacaat gagggaagta aatagtgcct tcagcgtata agtttctggt
     7081 gacaagtaaa tccctgcaat ggacagggta agggcaagct tcccacgttt tatgggatcc
     7141 agcttcagta gcggactgaa tcgctctgct attttggtaa ggtacacatc gaatagattt
     7201 tccttaccga taccggtctg ctttttggca agtaacatca tcttggatgt ctttggcgta
     7261 ggcacatcca caatggcaca ggatagatga tatagtccca gtgcgagcag accacaggca
     7321 gacatgataa gtagtgttgt cataggttac cttctttctt acttgtattc cacaggtttt
     7381 gacagtgcca tgatacgggt gaacgaaaaa agcacgatgg cagcacagac tgccagggtg
     7441 atttttccgg gaatggaata cataagtgtt tcaaaccagg agtgatttaa gacatacaaa
     7501 agagggatgt tgccaacaac taagaacatc atggcaaagg cctctttctt gggctcgttg
     7561 atgatcgctt caagctccga ctgaaggatc cgctgatccg aaaacttctg caccgtatag
     7621 gttaaggtgt ttcgcatggt acggtccgac tggcactgaa tcagggtatt acaccactca
     7681 tgaaagatct ggtttggaat cttcatcttc aggttgttta gcgtcgagat catattggcg
     7741 tttatcatct ctgattctat gatgaaactt tcaaaatgcc cctttaccgg aggatttaag
     7801 tacggcagat tttcctttac cgcggtcagc aggtcaccgc ttcgtaagta cgaggtggta
     7861 atcacagaga tagcagtttc cagctcctca tttaagtgct tcttatagac cgccgcggta
     7921 cttcgtaagt accagatcgg agtaaaagca aatccggctc ctaaaatggg aaccataaac
     7981 ggattgtcaa aaaggatcgc taagagtgcc cctaaaataa acaaaagaag gcagatacgc
     8041 tttacagcag agaatcttgt ggctcttccg gtggctacaa ggagctgttc tacctctaag
     8101 acctcccggt taaagaatcc cttagcaggt tttccaagga gaatgtccat gtcatctctc
     8161 agagtgctct tcctactggg ggagaatact ttaagaaaat ccgaaaagcg gacgttaaac
     8221 agggaaaaca gggaacttag tagtaataaa aagataatta tctgggtcca gtacacgcta
     8281 ctttcctcct tctttgcatg gggtatccgg taaaaatggt tttagtttcc cggcgggaac
     8341 cccgttgtct aacatccggc gctttaagtt atctgagatg ccgccaagct ttttatggga
     8401 gccaatgacg gtgatgttgc catatgcatc tgtgatattg tcggtgacat caaactggta
     8461 taagggatgg aagatgagtt ccccatcctt ataatcttcc ccttcgatga tctgcatggt
     8521 acgtctggaa ccgtcctcga gttgttttgt aaatacaaca atcggatagg cttccaccat
     8581 gatctgcatc aggatttcgt cagccatatt gtactttcgc tttgccagtg tcatcatgcg
     8641 acggtaggtg gactccgcgg aattactatg gatggtggta actaccgtgt gaccggttcg
     8701 ggagctttcc gcgacggaca aagcttcttt ggcggaacgc atttccccaa ccccgatcac
     8761 gtccggatgc ttacgaagaa cacgctctaa caaaagatcc tggtcgatat ccagggcagg
     8821 attttcactg ggacgggtaa gcaagtgaac cacactgttg atggcatgcc cctcggcatc
     8881 ccgcttgatc aaatcaaatt ccctactgcc ttcctcaatg gtaatgagcc gtctgttgtt
     8941 tggtaccatg gataagagcc agctcataag agtagttttc ccggatcccg tgcttccgga
     9001 gatgcagacg gataccccat aacggataca gcaggtaaga aaatcaagca tctcttctgt
     9061 ggcggaaccg gaggcgataa gcttatcctt ggatacggac tgttggttta caatacggat
     9121 agaagcattg atgccgactt ccggatctac gataggagtc ttgtccacgg agatacggat
     9181 attcttatca agaaatccga ttacggaagg catggtatca tcaattacca tgccgcaggt
     9241 gttaaggagc ctcctggtga catcaatggc agcctgggga gaaggaaagc ggtcaggaat
     9301 tttttccgaa tgtccgccac tggtaatgac ttcgatatca tcataggcat tgatgttgac
     9361 ttcctctacg ccgggcttat agatccattt tttcagaaaa gaaataccgg ccatatcttc
     9421 gtagagacgg tctaccagtt cttctgttgt ctgtccatct acgtgataag agcggttcag
     9481 tacatattgt aatatgtaat ccttcagtaa cttcctggct tcctctggat tcccttcgtt
     9541 tgatagggta tctgcgtgat tctgtgtgac gaatctctgg acatccgtaa gaatctgttc
     9601 ataggtaagc ccatccgatg ccttcgagga aaagaaaagg tcattcagac gctccatttg
     9661 tgctttcctc ctttctgacc ggtgcatccg taaattcttc cttggtagga tctatcagga
     9721 gactgtccgg gagatcctct ttggaatcgg aaagcttatt ttttacaagc ttcagagcat
     9781 ttaagtagcg ttgattgcag tagggaagtg cccggaacat ctgcccactg gtgccgcacc
     9841 gttcgatttc ctttgtgtag ggcaggatcc cgtcaaaacc accgatcaga ttatccattt
     9901 catcgatagc atgaaaaggt cttgcaagtc cggcaaaggt cagttgctca tcaaatttaa
     9961 acttactatc cgtaagcagg gctttgtgag ccctgaagta acttaagccc cgcaggtccg
    10021 gtgtaatgat ccggacaacc aggtcggcag cttccatcgc tgtcggcgca aaaaaattaa
    10081 gcatatttgc actgcagtcg atcaggatgt aatcgacaag ctttgcacat tcggaaatga
    10141 atattttgat tttctcgtat tttaattccg gataactaaa tggattttct ccggcggcat
    10201 atcctaagac tccgataaag gggtattcct ttaataccac aacacgctct gcaatcaggg
    10261 cgttgttgat ctccagagcg gttaagacat ttccgatgga tgcggaggtt tctaagatcc
    10321 ggtcaggcat ccagacagga agcatcggag tggaagagtc agcattgata atcagggcct
    10381 ttttcttccc ggcggttaaa actttcgcaa gattgcaggc aaacatggac tttccacttc
    10441 caggattccc ccagatggta atgatttttc ccatagtaac ctcctattct gcagcggcag
    10501 actgctcttc ggtagcacct gtggtctctt ctgcgtcagg agcaactgct ggcgtttccg
    10561 tggtaagaga tgaggtaagt tcctcttcgc tggtaccgtc tccatagaga gttaccagga
    10621 tgtctccctg ccttcctaag agatcttctg caagagcctc attaccacga ctgatgagag
    10681 cagcgtgaag aactccttca ttttcaaacc tggttaagag cagagcctgg acaggagtgg
    10741 caagcaccgt gatggtagcg gtctgttgtc tttcttcgtc ttccgcaggt ggtgttgtat
    10801 aatcaatggg aagaccctca gagtccgtaa ccccaagaac ttttacataa cgaagctccg
    10861 ggatatctgc cacaggttcc aagacatcct ctttgtcata gtggaagagc ctgataatgt
    10921 cattggcctg cagcttatcg gacaggccgg aagccagagt ctgggtggta atggaaatgg
    10981 caacataacc atccgggata tgattgaggg acagatccga ggataacgga tctttgctta
    11041 actttgtggg gagcagatag tctccggcat agagatttgt ggtggcatag gtacctgtta
    11101 catcggaaat ctccttggcg agaccatccg gcaagttgta gccacccact tcaacgagct
    11161 ctacgtcggc ggaggtgacg atacttcctc ttgctacgtc tgatttcata cgcacaatct
    11221 gcgtggtgtg gctggtttta ctggttacgg cgggaattgc tataaaggca acaatggcag
    11281 ccagaataag gcttaagatg ccataaataa aacggttatt cattttcata ggtttgtctc
    11341 ctgtatcaaa atagtgaaag aatctcataa aataagcacc caagaaacaa aaagggtgca
    11401 aaggggatgg cagtaggttt tcttctgtaa tgaaggcgca agggcagtgt tgcaaagatg
    11461 gcggctgtgc cagacaggat aagaatctgg cacagcccca aggctccgta gtagagaccc
    11521 aaaacggtta caagcttgat atcgccgcca ccgattccac cattggttgc aactgcaatg
    11581 atgagaagta agaaaaaacc aacactgcca cccagaccag tcttaaaaat aagaagtggc
    11641 agggaaggtg cgatacccag gataagtttt agcaggatac cggatagaca ccacagggat
    11701 aaaaacacca gggcagtgct ctttatcaga tggtgcctga aatcgtagat cccgaataaa
    11761 agaaggaaca ccagggtaag gatccggtag attaggtaaa ggctactcac gcttactata
    11821 ccagtcatca tacatggagc cctgaatata tacggagtcg gtaactccgg tagagagcat
    11881 tccggatggc gtccacatat ccatcacgta ggtatacacc ggataatagc cgtccgggta
    11941 ccagacggga gtgaaatgta cgtgccttga gtaagtcgag tattcatttt ctttgaagga
    12001 aaaggtattt gcaccggttt tctcaagtag acgccagtag gtatcatagg aaaactccgg
    12061 gaagtaggat accgcggact gaacttgcgt atagtggctg tccggcgcat tgaccgtgaa
    12121 gcttgcattc agaatttctt tgatgccgta cccacttttc atggtgtcgc cgctttttgt
    12181 ggggacaata tcatcgggag aaatatctga ggatgcaaaa agagctgcat gataacctgt
    12241 ggaggtaaaa tcataccagc cattatcgac ccactcccca tcatccaccc attcgccatg
    12301 ggcagtagta cagcctttgg ggcagctttt gctatgtccc tccgattccc agtgccagtc
    12361 gggttcccat tcccattttg cgtgccagga tgcactccag acagaccagg aggcatttgt
    12421 ctttaccggt tctgatggga gcgtggatgt ggaaaatccg ggattggtat ccgtagcaag
    12481 aggatccggt ggaatgtttt cctccagaga aacaattctt gcaaggaatg aagtttttgc
    12541 agttcccaca ccactgacag agacactgat cttgacagtc cccgggtctt ttggtgtgtg
    12601 ccatttgacc cagacaagtt gcgaaccgtt ctttggcata acaatatcac tcaccgtata
    12661 ggtcctgccg tcaatataaa aggtagcact tgcaggatcg tcgggattta cttctgtaga
    12721 actggtaagt acaatgctgg atattacatc cgtgtctacg cggtaggtgt aatccggagc
    12781 ctctagctcc tctgcaggaa cgtctttata acttacgata ccgataccta aagacgataa
    12841 gatggcatca tccgacactt ttccgctagt gcttccggtc catgcagggt atccgagatc
    12901 agatttttct aaaaacagcg caagcggcag gttctggtgt gtcagggagg gaagcacctt
    12961 tcttaatgca cctccggata acttgttgta aagggcagcc tccgttgctg tcatggcata
    13021 gttttttcca ttgtgcgtga agtaggcaat gggttcgatc accaatttgt aatccccatt
    13081 aatcagattc tcaaagggga tgccggtttt ggatgcgata aatttagctg catactctga
    13141 gcagatgtag cgttttatcg cagcaatgct ggctttgttc acagatccgc tgatgattct
    13201 tggtagagcg acggctggct ttgagtaggt gtaggaggca ttaatggaaa gcgatgagcc
    13261 cgcagtatat tgcagtttgg acacttttcc aaagtttaac accgtggagg aaatgttgca
    13321 gtttgataaa tcaatgggag tagcaacaac gctgcctccc gtggtaacga ctgtaacccg
    13381 aacaccatcc tggttatttc gccagacgtt tagttcggat cctgtaccaa aagatccacc
    13441 gcctccatca atatttccat cacccatagc attagtggaa atgcctgtgg ataaaaatat
    13501 cgttagggat agaagcagtg ggaaaaagta tttcctaagt gctgtcataa gatctccttt
    13561 gcaataaaaa agacatggcg tgcttaccat gtcttagtcg ttttttgtta ttcacccata
    13621 gtgcctattt gcttgttgat gtctccatca ctgtcaacca catcataagt agtattaccg
    13681 gtatcaatcc aaccaaatac cggatcataa acttgtccgg gatgggaact ttctgaatta
    13741 tcagcaggag tctgcacttc tggtgccgtt acaggaacct cttccggata ggaaggaact
    13801 tcgtccttat ttgtggtatc cccaatgggc accggtttat cggtaggttt ggtctcctga
    13861 gcttcctctt ttgtcacact tccggataga tctacggtgg atccattttc atcttcagat
    13921 atgacagtct gagtattgtc ggatacttca ccggtataaa gaggttcttc tttgacagat
    13981 gcctccggta catccgactc taatttatca tcccaggtgt ctgccagggt ttctacttcc
    14041 tgagtggcag gagtgaactc ctctgttggt tcatagttta aataccaggt gccagcaaga
    14101 agtaccaggc acaatgtaca tagtcctccg atggaaaata cgactttttt atcaatgttc
    14161 ttctttttca taggcggctc ctttcttggt ctttaattac atatacgcaa aacagagtat
    14221 aaatcttaca gaaggtcagt attttgtatt atgggatccc gttaattcta cgtgccttcg
    14281 gatcggaggg aaagactctc caaacattcg gatccggtat gtaatgtcga acgatgcga
//
LOCUS       DAWEJW010000073        14132 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1562_length_14132_cov_68.225829, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000073 DAWEJW010000000
VERSION     DAWEJW010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14132)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14132)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14132
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1562_length_14132_cov_68.225829"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10818..10917
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3285 a         3801 c         3052 g         3894 t
ORIGIN      
        1 ctgataccat cctgtgcatt cagggaagcc tgtgtcagac ctctgatctg ctttctcatc
       61 ttctcactga tggacaggcc tgctgcatca tcagctgcac ggttgatctt ataaccggaa
      121 gacagtttct cggtggattt tgcctgagaa gcggtggtca gacctaacat tctgttagag
      181 ttcatagctg taagattgtg ttgtactacc ataataattt acctccttgt attactgccc
      241 aattcctttt gcggcagtgt gcgtatctgt attgttattg agaaatcctt ttctcccaga
      301 tacttgttgt tagttttata gtaaccggag taggagcttc cctgtcgggc cctgacagag
      361 ccgctcttcc tccggttatt accatatgaa attatttgtc cgcatttccc tttgtgatga
      421 tcacgttgga ttttttgcgg tacttctccg ggtgctcttc ttccgcataa tatttaggaa
      481 gcttcgctct ctcctccgga tctgtgatct ggttttctaa taccgtgctt cgcacgatgt
      541 tcacctcttt cggtgcatcg atcatgatgc ggagatgatt cttggttcct cccaggaaca
      601 cgatcttgat attgtcattg atcatcaggt attcttccgg actaacggtc agacgcagca
      661 tataataacc tccttgctct gttcagcctt ccttgcctgc tgtttctcac tgatgcaaca
      721 ttctattatg gaatgttgca ttttgtcttg cactacatat ttcggctgac catgtgaaaa
      781 ctttagtctt ttttataaaa atcgctaata cgttatttct atattattat agcaattata
      841 aaggccgcat gtcatagctg tatgtcacga aatacggcat ctttgtcatc ctgttcaatt
      901 cccagataat gttttgtgat ctgaaaagaa gaatgattgt aaatctccat cagcaatgtg
      961 ggcggagttc ccattttcca tgcatggtag ccgaaggtct tccgcaggga atggcaccgg
     1021 atcacgtctt cgatccctac ggcctcggca gccttgcgga caatgcggta ggcctggtat
     1081 cgggagagcg gggaatcagc ttttctggga ctgggaaaga ggtagtctgt gtcggctatg
     1141 gaaggctgtg ttttccggta ggacagtaat gtttcctgaa gttctctgtt gatcaggatt
     1201 ctggtttctt tttccgtttt tttctccgtg atcaggagat gggtgtaaaa gcggctgtgt
     1261 tctgtgcaat agacatctcc ccaggtgaga tgaaggatgt cgttgatgcg gagagcggtg
     1321 ttcagaccga tcaggatgag agtgtaattc ctggagttgg gctgcttggt aaggaagtac
     1381 tccttgaaat cgtgtagaag ctgtttgctg cggataggtt gtgtcgtgct cataatggcc
     1441 tccttgatag aataaatgaa ttagggaaat gcaacattcc ataatagaat gttgcatttt
     1501 tctattttaa tcctttcatt tctgtggtat tcttttaaag actgcatatc gctatttttc
     1561 gacaaatact ttcctttttt gaaacatata cagtcagaat caggagtaca catgaaaagc
     1621 tttatatgtg aaatgttcta taagcagcgg gcggcattcg aacaaagctg cgcttccagg
     1681 ggtaaagaat atcctcttcc ggaagatgtt ttcctacaat cggatatttt ctatgacgag
     1741 aagcatgttc cggcacatcg tctggatgtc taccgtccca ggggacggga cggagagatt
     1801 ctcccggtca tcgtcaacgt acatggcgga ggcctcctca tagggaacaa ggaattcaac
     1861 cgtccctttt gtgcctccct ttgtgaggcg gggtatctgg tattcagcat tgagtaccgg
     1921 ctgataccgg actgtctctt ctatgaccaa ctaactgacc tgcatctggc actggatttt
     1981 atccgggatc atctgacgga atacggcggt gacatttccc acatatatgc ctgcggtgac
     2041 agcggcggtg cctgtctgct gacttacgga atcgccatgc agcgaagcac tgcactggca
     2101 caggctgccg gagtaacccc ttctgacctg ccagtccggg cacttggcct ggtcagcggt
     2161 atgttctata cgaaccgcct ggatcagatc ggacttttcc tccccagata tctctacggc
     2221 cggcattata aaaaaagtgc ttttgccccc tatgtatctc cggagcaccc ggacatcgtg
     2281 accagcctgc cacccagtct cctgatcacc agtgacaacg ataatcttca gagctatact
     2341 ctgaattttg aaaaggctct gcgcagacat cagatgcccc ataaactgat caactacccg
     2401 aaggatcccc gcctgaccca tgcgttcagc gctttttacc cggatcttcc ggaaagcagg
     2461 gaagtgatcc accatttgat tcattttttt gaacataccg gagatgactg taaaggagga
     2521 tacgtatcat gacatacgac gaaaatgttt tcaaagaaaa agctaatcgg aaagcccgta
     2581 agatctggat cgtttttgct gttttactga gtgccaacta tggctccgat gcgtccgggg
     2641 gattatatcc caccacttct taccttattt tcctggcatt gtgctggctg cctctgatct
     2701 tcggcgaagt gctgttacgt gtaaagggct gggcaacgga gctctaccgc tacgatctgg
     2761 tcatcggtta cggcatcttc tatacctttg taatctgtac cacagcgtca cccatcgcat
     2821 tcacttacat ccttccggtg acaagcttac tggtcctgta taagaaccgg aaattcatga
     2881 tcaactgcgg tatcgtcaac tctctgatca ttgtgggcgc tgccgtctac cgctacatgc
     2941 tgggctttaa cagtgcttcc gatatgaaga attaccagct gcagctgtcc tgtatcatcc
     3001 tgtgttacat ctgctatgtc atgtccatcc gccatctgaa cgagtccgac ggtgccatga
     3061 ccgacagtat caaagccgat cttcacagag tggtgaccac cgtagaacag gtcaagacat
     3121 ccagcaatac cattctggac ggcattaccg ttgtcagaga actggcttct gagaacaagc
     3181 acggttccga tatggtcatg ctgggtatga atgaattgac ggataataac catatgctac
     3241 aggatcggac gacctcttcc acacagatga cttccgacat ccgtgcccag gtggaaaatg
     3301 ttgtggcact catcagcgaa atggtatctc tggtcgggaa aaccgaatcc cattccagtg
     3361 tcagtgcaaa agatctgcaa agtttggtaa gcactgccac cacgatgtca gagctttcta
     3421 cggagctgga aaatgtcctg cagaatttcc agcaggaatt tggcatggtg aagcaggaga
     3481 ccggtaccat cgaaaaaatt acaaatcaga cgaacctgct ggccttaaac gcttccattg
     3541 aagctgccag agccggtgaa gcaggcaagg gcttcgcagt ggtagctgat cagatccgta
     3601 ccttaagtac tgaaacccac gcttcctccg gacagatcag agaagctctg tcccgtctgg
     3661 atgctacttc cgctaagatg accgcttcca ttgaagagac cttgaagctg atccaggtta
     3721 ccctggaaaa ggtaacacat accggtgaaa atgtaaatca gatcgcatct gattccgcac
     3781 agctcggcag acatattcag gtagttgata acgcaattaa agaagtcgaa agctccaata
     3841 cccagctggt atccaatatg gaacaggtat ccaacatcgt ggagactatc accggctgta
     3901 ttgcacactc cagccagacc agtgagcgta tgctcagtaa atacgaagaa accgcttcca
     3961 atatcaacac catcgaggat gtcatggaga acatgatgtg tgatctgggt atcggcggct
     4021 tcatgggtat cgaagatatt cagccaggta tgaagatcga tctgaaagta gccggccagg
     4081 gagatacgga ataccttgga gaactgatcg aacagattcc ggaggggctg ctggtatcct
     4141 gtcagaagga gctagcactc acagataccg cttcctgtac cttacagatc accgcaggca
     4201 acatcctgta ttgttgggac aaggcagtca tctctcccgc tcctgaaaag ggtgctcatg
     4261 ccttcaaaat catcattaat accagaccca ggatcaataa ccgccgtaaa tacccccgta
     4321 tggatctgaa caatgcctgc accatcaagt tcaaaaattc tgatacggaa tatgcggcca
     4381 ccatggataa tatcagtgcc aacggctttg ctttcctggc gacagacaat atcttcaccc
     4441 agagcaagaa tgcctcgatc actgtcacta tccatgattt cgctctcccg gatcacaatg
     4501 tgctggaagg acgtatcatc cgctgctccg atgataacgg tctgttcatc gtaggctgcc
     4561 agatgccgga ggataatttc tatattctgg agtatgtaga gaaaaatctg gaaagtaaga
     4621 aatagtcaaa tacaaaaggg tgccgctgtg cgacaccctt tcttgtttgg ttttatagtt
     4681 tcatctatgg cttcaggagc cgggcgatca ggccctcgac ctcctggagc tgttcttttg
     4741 tgctctcctt gtggctttcc ttgcggtact tcgaagggga cattcccttc atggcgtgaa
     4801 aggcctttgt aaataccagg gcatcactgt atccgcagga caatgcaata ctctcgatag
     4861 gataattcgt ggagtccaga agttctctcg ccttggtaat acggaaggta gtaaggaact
     4921 gctggggaga catccccaga tagttctgga acagcgtata taaatagctg cggttgatac
     4981 acacatagtc cgccacatcc gtgaccttga tggggttaca gtagttgctc tggataaagg
     5041 agaccgcttt tttcacatac tggttcccct tatcctcttc ctcccttgcc accacaccgg
     5101 cgctgtcggc gatcaccgac agaaatactc ccagcagtcc gttccgccgc agatcatccg
     5161 caatgccaaa ggtattatgc tccatcatat cccggacaat ggaatatagt tcctctgatt
     5221 tatcacatga aaaaatagga tgtctgtctg acagtcccat gctatgtaaa tattcttctg
     5281 cccggcttcc ggcaaatccc acccacagat aactccaggg atccttcgca tccgcctgat
     5341 aaaaagccag ttcattgggc tggatcagga aaccgtaacc cgcctccagc cggtattcct
     5401 tgtcgtgaaa ccggaaatgt cccttaccac tcaggacata gtggatcagg taatgtggct
     5461 tggaggccgg tccgaagctg tgcagcggtg ccgtctggga gcatccgcag cagttcacat
     5521 acagacttcc tgttttatca ttgacaaaat ccagtttaaa tgctttttcc atgatcggac
     5581 cgtttccctt ctgcgttgcc gctctctcat ctaattcacg agaatcccgc cgttttcatc
     5641 aaattcgaca ccgaaatccc ggctgtattc attcatacgc tgcaaaattc tcttcttatc
     5701 ctcacctgct gccggagtag gcttaaaatc attccgggtg gacacagaac tcaccatgca
     5761 gggaatgatc ctggcattcc ggtcctccac cttctgtccg ttttcgaatg taaaggtctg
     5821 ctggaagatc atggtatcct tatctgccgg gttccggttc gctccgaagc agaaattcgc
     5881 caggctatag atgatatatc tgcccttata ttcttcgatt ccctgaagga cgtggggatg
     5941 atgaccaatg accagatcca caccccagtc aatgcatttt ctgccaagta cctgttggta
     6001 attctccgga taagtatctt tctcgatccc ccagtgacaa caggccagga tcaggtccac
     6061 attctgttcc cgcagggtct cgatattgtt ctggatcagc ttttccgcct gtgcgcccca
     6121 ctctacctca ttgacagaga ttattcctat tttcaggttc cgctctccgc cgtgggctgc
     6181 cgcactgtcc gggatctcat agatacccac atttttatca taagcataaa tgatcccttc
     6241 ctgctctaat gccaccacag tatcccggct tccctgttcg ccataatcca ggcggtgatt
     6301 gttggcgaag ctgacggcct caatgctccc cagcgtcagc agatgagcat aggctggatc
     6361 ccctttgatg cagtaggtct gtccctcccg catctgttcc gatgtggtca gaggcccttc
     6421 cagattcacg atggtcatat cgtccgccgc aaagatgtct ctcacatttt caaagaaata
     6481 accttcgtcc tccgcctgat cataggcctg acggaaggag gcactgtagt cttgttcctg
     6541 atgatttccc aacgttacat cccctgctgc ggagatggtg atcgtataac gttcacccgt
     6601 caaatcaccg gagtcatggc aagatcccga tgttgttgcg ttctctccac cggaagccgt
     6661 ccctgtctgt gcccccgtag cagaatcttc tgccgtctgc ccagcctgcg tagttgcatt
     6721 gtaaccgccg ataggctgcg ccagatcttc ccttcccgga atgtagacct gctgggtcag
     6781 atcatcgcca catccaccaa gtattatgac tgccgctgca cagaacagtg ctgcaaacct
     6841 ttgttttcga taccccataa gctgctccct cattatcgta gcatgatccg catctttggt
     6901 gcttaccgtt gcttcgcaac tatcatgatc ccattcgccg ttcgtcaatc ctgcaagcac
     6961 attgactctc atacgtcaat tataatatca ttccgcagaa gcctccgatg tggaagtgat
     7021 gtctccgtct ccgtacagat tctcctgcag gaatttcttg ttggcttcga aatccacttc
     7081 cagcactgcc atcttacgga tggtcgcatc cttataggtg ccctccagtg gaatgctgtt
     7141 ctggataata tcgtaagtca cggccttagg tgccataagg ctcagccgat agcattctgt
     7201 ctgggtcaga ttcgtggtca gattcggcat cagaccatcg atcagctcct ggggattggt
     7261 cagcattccc tgcggaagct tgtggatcac ctctgccaat acctttctct gcctctcggt
     7321 tctgccgaaa tccgtaccga tataacggtt tctgcaatac gccagtgcct gcggtccgtt
     7381 caggtgtacc ataccgccgg aagtatcttc aaaatagtcg gtaccttccg gtcttcccaa
     7441 cagaatgttg tattccacca gatagccgtt gacatattcc acttccttgc tggtcagctc
     7501 aagatccaca ccgcccactg catccaccag attggcaaat gcttcaaagt tcaccagtac
     7561 atagcggcta atatgaatat cgaaattctg ctcaatagtc tccatcagca gttctgcgcc
     7621 accataggaa tatgccgcat tcagacgatt gcccttatgt cccggaatgt ccacatacat
     7681 atcacggagc agggatgtca tgtagatctt cttcgtcttg ttgctgatgg acagcaggat
     7741 catggcatct gatctgccgt cttcaccgtt ttcacgggaa tcgttaccga tcagcagaat
     7801 attggtcacg ccttcttcct tcatgggaga ggacgctacc gactcgatct tctcatagtt
     7861 catctctccg tagacctttc ctaccagcac atacaatcct gctgccagga atacaaggat
     7921 cagcgccact gcgatcagga aacgcttgag tcctgacttt ttcttctttc tgccgctccc
     7981 ggctcccttt ttatttcctt ttctgttcct tttatccgct ttcgtgtcta tctcctcacg
     8041 ctttccggat cttcttccgg agcgttttct atcgttataa tcgtcctcat agtcctctac
     8101 atagtcatcc cgtctgctgc ggcgggttct gcctgatgtc tcctgtctgc ggtgatcagc
     8161 gttactgcta cggccgccac cgttacgact ctctgaagtc ttacgtgccg tctctctgcg
     8221 ggctgcctgt ctcctgcggt atgtgggatc atcccagtcc tctgcatagt cctcacggga
     8281 tcctgctctt ctgtcggatc cgatcacatc cagttcctcc tcttcctcgt catcttccct
     8341 catatcatac atctgcggag ccgggcgggc tgcctcacgc tccgcagagt gtgtccccgc
     8401 attcgccgcc gggcgccccg tactgcggcg ggctcccgcc ccggatgcct gcatttcctg
     8461 catctcctgt tgtaaaaagg cttccagacc cttttgtata tcttccatct cctgaatgga
     8521 gtctctttcc ttatcgctca tcctgtacct cacttccaca tacctgattt tgcactcttt
     8581 tattcctatt catcttctag gataatacca taatctgctg tattcgtcac gacagaaatc
     8641 cttaaaattt gtttaaatgt tatccggtat ttcttaagaa acgccaacaa gaaagaccat
     8701 ctgcggcagc tacacgattt tctttcgtat gctttcctca gatggtctct gcatttttgt
     8761 tgacttatgt ctgctttatt tatgagcaag ccttgcgtct atgtaatttt tcagagattc
     8821 caccgtctct tctacaccca gcttgctgct gttgatagac agatcgtaat tacgggaatc
     8881 gccccatttt accttacaat gactattgtg gtaagacttt ctgcgacggt ccttctcgat
     8941 catgcgctct tccgcatgac gggcatccaa ctcatagatc cgcataacac gttcgatctt
     9001 ggcctccctg tctcccaata cgaagataga gatcaggtgc ggattgtcct tcagcacgat
     9061 ttcggaacaa cgtcctacga tcacgaagga ttctcctgcc tctgcttttt tcttaatgta
     9121 atcgaactgc attctggcca cattgtctgc cggagagctg ttcattccca ttacactgcg
     9181 gtacaggaat ttattacgtc tcttttcgtc gaactcagcc agctccttgc tgcttacatt
     9241 catgcttgca gccacctcat ctaacaggtt gtgatcgtac atcggcagtt tgtaatactc
     9301 tgccagcttc tgtgcgatct catgtccgcc gctgccgtat tcacggctga cggagataat
     9361 cagttgttca ctcatggatc tgccctcctt atggctggat gtatttccaa aattttttaa
     9421 actcgtattc tgcaaataca tcttacagat atcttgcata gatcatcagc atagagatcc
     9481 ctactactat tatagtcgca ggtgccataa ctttgcaata ttttccccac ttaatcatat
     9541 gaccggcttt cgctgcggtg gcgataccta ctacgttggc agaagctccg atgggagttg
     9601 cgcttcctcc aatatcggtt cccatagcca gtgcccatgc cagaatggac agctccacac
     9661 cctgggtagc ggacagactg ctgataatag ggatcatggt agccgcaaaa ggaatattat
     9721 ccacgaaggc gctggcaatc gcagagatcc acaggataat agcgatcatc agcatcagat
     9781 tgccgttgct gatgtctccg atgaaattcg ccataacttt caggatgccg gtctgctcca
     9841 ggccgcccac taccatgaac agtccgatga agaacaacag ggtcttgtaa tcgatctgtt
     9901 tgatcagttt cagtgcgtcc ttgcctgctg caattaaagt cacgatagaa atgaatacac
     9961 caatacagga tacggtcaga ccggtttgtg cgtgggtcac cagcagaact accgcacaca
    10021 ggaaaataat agtgctgata ataaatcctt ttttatcggt aatggcttcg gaaggatctg
    10081 ggtaggtctg gttgctgcct gctgcagctg cctcactggc acgcagttcc ttgtggaata
    10141 ccagatagaa atacagtacg accacaacca gggaaacgcc tgcgatcaca ccggtattgg
    10201 tcaggaaatc cgtgaaggaa tatcccaggg aagtaccgat gatgatattg gggggatctc
    10261 cgcacatggt agcggatcct cccagattcg cacagaacac ttctgccaga atcataggca
    10321 ccggattgaa tttcaacagt tgtgacaatt caatggtaac ggctgccagg aacaggatta
    10381 cggtaatact gtcaatgaac attgccagaa taccggataa taccataaag gtgacaaaca
    10441 gcggtacaac cttatacttg accatcttgg caaggcgcat gcacagccag cggaagaatc
    10501 ccacccgggc cattccctct accatgatca tcattcctgc tacgaacacg atggtctccc
    10561 agttgatacc actggaagcc tctgcggact gtcccgccgt gtaccagaaa tgacttgtga
    10621 aaaagctgcc taagttcaaa gtctccaaca ctgcgctcat actgtgcatt cccagtccga
    10681 ataccagtac cagtgtcagc aggccgcagc ctaatgtgat aatgtgtctc tcccatactt
    10741 ccaggacgat cagtacaaac atcgccagaa atatgattac tgcaagtatt tgtgctacca
    10801 ctttccctgc ctccttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg
    10921 actgtgaagc tccgtacact cctcttcctc tctgccaagc agcctctcca tggcaacgga
    10981 acgtaacagg aagcagcaga tgtttgcagc attccgcata agctgcgctc tggtcagcct
    11041 gccgtctgcc agtccttccg cagtgttgtc cttattagaa ttggatgctg cgtctgcggt
    11101 caccatgtaa atatcattct gcgcccggac cataggtacc gtctgactct tggaaccgga
    11161 ttcgccttcc tcattgatct ttgcccacca gtcggtcatg acaataccct gatagcccca
    11221 ttccttacgg agaatggttg tcagaagatc atagctgcct gcggtccaca gtccgttcac
    11281 cgggccatag gtggacatga tgctgtaggc tccgccctgt tttacggcga tctcaaatcc
    11341 tttcagataa atctcccgca acgccctctc ggatacaata gcattggcat catgccgttt
    11401 gaattcctga ttattacatg caaaatgctt gatggtaccg gtaactccgt attttcccat
    11461 acctttcagc tgtgctgccg ccatggtgcc ggtgaggtac ggatcttcgg aaaaatactc
    11521 aaagttacgt ccattcaacg gatttctgtg aatattgatg ccggggccta agagggaatc
    11581 aatcttgttc ttgcgaagct ccatgccctc catctcatac agttcttcca ccaactccgg
    11641 atcaaaggta catgccagca acgtaccgtt cggcagtgca taggccatgg taccgcagtc
    11701 catacggatg ccggaaggac catcggaaca gcaggccacc ggaataccga attgctgcag
    11761 tccatccgtc acaccgccga aagctgctgc cgttccaggt gttactttcg gagaacacat
    11821 gccttctcca cgggtcatac agatcagatc ttcgtctgac agctgtgtca gaaactgctc
    11881 cggcgtgatt tttttgtcgt aaacatccgc cagtttccag ccctgatctc ccacatattc
    11941 gctctgggta ggtctttccc gcaaaatacg ttccgccaga tctatggtcc gtaagggcac
    12001 atcctcccag ttggcgatgg catggtcatt ctctccgaag aaaaacggtc tcaggcgctt
    12061 aaatgcctgc accggagcca gtgcttccgt aacggtttgt acgacctgca gctccgctaa
    12121 tgtcactctt ccgacttctc ttgcactccg cacatcggta ccgacataga agatatagtc
    12181 tcctgcttcc aatacatagc aggatttatg tccagtgaca ccactgtcat cataggacgc
    12241 aagctccgtc agttctgctt tcaggatcag ttcttcactt gctccgggct tcagttcttc
    12301 cgtcttggca taagctgcca gctgacgcag cggctttccc agctttccct gaggagcctc
    12361 cacatagacc tgtatggtct ctctgccgga cataccgcca acatttgtca ctttctcaat
    12421 gacagttgcc tcggtaccct gcactgccag tcccatgctc tctgtctgga accgggtata
    12481 ggacagtcca aagccaaagg gatattttac cttgtctctg gcaaaggtct caaaatagcg
    12541 ataacctaca tagatgtcct cggtataaaa attctccacc ggatccccga aattctccgt
    12601 ggaaggataa tcctcaatat cttccgcaat agtatcgctg agcttaccac cgggattcac
    12661 ttttcctgtc aggacatcta ctgtagctct gccgccctcc tggccgccct gccatacata
    12721 gagcacagcc tgtggctgat accggtcgac ccacttcata tccatgatat tacccacatt
    12781 cagcacaacg atcgtcctgg caaatgcatt gcagacatta cgaagaattt cttcctccgc
    12841 agctgtcagc agataactgc cttccgcagc actattatcc ttgtcttctc ctgcggttcg
    12901 gccgagaacc acaatcgcaa gatccgactg ttccgccgcc tgtctcgtca gttcttcgct
    12961 gacgggcatt tcttcctggt tccagggctc catagcccat ccctctccta tatcaaaagg
    13021 atgatccttc agccattccc ggtactgtgc ctccagtacc tcattcacct gtacggtgcc
    13081 gtcttccttg agactgtctg taatattggt cacatatgga gcattgacca tacctccgga
    13141 acccgtaccg ctcttataat gctcgaactg ggttctgccg aagatcgaca ctttctgccc
    13201 tttttgcaga ggcagcacac cgtcttcatt tcgaagcagt acgcttccct ccgccgccac
    13261 acgtcttgcc agttccgcat atttctcata atccaatgta tatttcatat atctgtttgc
    13321 tcccttcctg ttatatctat atttctgtat cttttatcct aataatagca ttaatttccc
    13381 aatacatcca caagaaaagc tctttctctg acagaaagag cctcaaaaac attttattga
    13441 ttttacaaga tgatccgaac cacaaaatcc ccatcctcca taaaaaactg atattgtccg
    13501 ctaagttttt ctacataata tacgatgctt ctggctccca gcccgtgtcc cgaacgttct
    13561 gtcaccggaa cgccatccgc aaatgtggga acactacgca ctgtattttt ctccatcaga
    13621 agaagatgcc ccgccttttc cgacagagac aattctatgt atcgtttatc ctccggcagt
    13681 tcctgtacgg catgcatggc attttccagt acgtttgaca ggatcgcaca gaacatcatt
    13741 tcagagcagg gaagtttctc tccaatgctg acctgtatct cccatcggat cctctgctcc
    13801 tcaaaccgca tattatagat ggacagtacg gaattcacca tctcatttct acaataggtc
    13861 ttgatcactg tatcatcata agtctggctg acctcattca gatattccag cgccttgtcg
    13921 gtttctccct gttggatcag tgttcgcagc accgacagat gatgcctcgt atcatgccgc
    13981 aaaatggaca tctttttctc gctttttctc gtattggtca tttctgtctg gaatgaattc
    14041 agctgcatct ggatcagttc attgtactgc tctgccttgt tcttcatctc gtactcccgg
    14101 aaatatacca gtaaaaagag cagatacgtc ag
//
LOCUS       DAWEJW010000074        14090 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1567_length_14090_cov_58.330958, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000074 DAWEJW010000000
VERSION     DAWEJW010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14090)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14090)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14090
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1567_length_14090_cov_58.330958"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6428..6527
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3887 a         3076 c         3619 g         3408 t
ORIGIN      
        1 gataaggata agatcgcact ggtaccggat cattttgttc ccaataagga tattaagtct
       61 gcggagcact gcaaatgtgt ccgtgagttt gcgagacgca atgagatcac gaattatttc
      121 gaagtaggag agatgggtat cgagcatgcc ctgcttccgg agaaaggact taccgtagcg
      181 ggagatgtga tcatcggtgc agattcccat acctgtacat atggtgctct gggagcattt
      241 tccaccggtg tgggaagtac cgatatggca gccggtatgg ctaccggcaa ggcatggttc
      301 aaagtacctg cggcgatcaa attcaatctg accggcaaac ccgcagaatg ggtcagcggt
      361 aaagatgtga tcttacatat catcggcatg atcggtgtgg acggtgcatt gtataagtct
      421 atggaattcg tgggagacgg tatcgccaat ctgtccatgg atgaccgctt caccattgcc
      481 aacatggcta tcgaagccgg cggtaagaac ggtattttcc ctgtggatga gaaggctatt
      541 gcttacatgg aagagcattc aaaacgcccc tataaggtat ttgaagcaga tccggatgca
      601 cagtatgatg cagaatatac cattgatctg agcactttac ggcctaccgt ggcattcccg
      661 catctgccgg agaatacaca taccattgat gagatccacg agatggagcc catcgctatt
      721 gatcaggtag tgatcggatc ctgtaccaac ggacgtatcg atgatctgcg caccgcagcg
      781 aaggtgcttg caggcagaca tgtggcaaag ggcatgcgtt gtatcgtgat ccccgccacc
      841 cagtccatct acatgcaggc aatggaggag ggactgctta agacctttat cgaagcggga
      901 gcagtggtaa gtacgcctac ctgcggaccc tgcctcggcg gatacatggg catcctggca
      961 gagggagaga gatgtgtctc cacaaccaac cgtaatttcg tgggacgtat gggacatgta
     1021 aagagtgaga tctatctggc gagtcccgca gtagcagcag ccagcgccat caccggcaag
     1081 atatcctgtc cctgcgaact ggagtaagct gcaggaaaaa gcaagatgcg ggcaggcaag
     1141 taagggattg tggaatcctg tggctgcctg catggaaaag taagagatat attggaacag
     1201 gaaaaatatt tgaaacattt gtaatagaaa ggtaacggaa aatgaacaac gcaaaaggaa
     1261 tagttttcaa attcggagat aacgtggata ctgacgttat cattcccgca agatatctga
     1321 attcctccga tcctgcagaa ctggccactc actgcatgga ggatattgac aaggacttta
     1381 ttaaaaatgt aaaaaagggt gacatcatcg tagcggagaa aaacttcggc tgcggctcct
     1441 ccagagagca tgcccccatt gccatcaagg cagcaggcgt cagctgcgtc atcgcagaga
     1501 cctttgccag aatcttctac cgcaatgcca ttaacatcgg actgcccatc attgagtgtc
     1561 ccgaggcatc ccggaagatt caaaacggcg atgaagtcga gatagatttc aataccggag
     1621 tgatccaaga tcttaccaca ggcgaaagct tccagggaca ggcgttcccc gagttcatgc
     1681 agaagattat cgccgcagag ggactgatca actacatcaa cagccaggaa taaattgagt
     1741 gcatggctcg taacgtaggg ttgccataac ccccacgaac gcccgcagcg gatggaacat
     1801 ccttccgggg tgtcaggtct cccggaggga atttcctgac gaaaagtgac atagcggtcg
     1861 agaaaatatc accatcgtcc gacaaacatt cccggaagga cattccatcc gctgcagggg
     1921 ttctacattt tctcataata tctgtacgtc ataggtattt ctgcctacag ccatttctat
     1981 tgttttggtg caaatcgcat caaaatagca aaatcagtac aaaaaatctt ctaaatcaaa
     2041 cattttttgt actgatttta ttatttttcc cattttagcg aaaataagct cttataaaag
     2101 gccaaaatct cctgtttttg tattttttat gcaaaaaaag gcgtttttaa tacaaacttt
     2161 attgtaccac tgccttatcc acatcaatca ctgcgtagaa atccgggtgg ttcatctgga
     2221 tccgtttcgt gacggagtca accacatcct gatcggaaac gggataatca taggggacta
     2281 cgatgtcaaa aatgaggttc gtgtgggtca gtcccttgac gatgcggaaa tcatgcatgc
     2341 tgagttttgg atcgatctga tccagataag cttttacctc tgctttcagg gacagtgtct
     2401 cggggtcatt ggtgctgacg ggatccatgt ggatcacagc gttacagttt aatttgctgc
     2461 gcagttcatg ctctatggta tcgatcgtat cgtgcagggt cagaatatta ccgtcggcag
     2521 gaacctccgc atgcagagag atcagagtcc ttccggggcc gtaattatgc acgatcagat
     2581 cgtgaatccc ggtgacatca tcataagaga gaacgatgtc gttaatctgc tgtaccagtt
     2641 caggctcggg gggctgccca agcaggggac tgatcgtgtc tttggctgcg acaaatccgg
     2701 agtacaggat gaacataccc accagcacac cacagtaacc gtcgataatg atgccggagg
     2761 cggcagagac gatagtgccg atgagaacca cggtggttgc tatcatatcg ctcaaactgt
     2821 caatggcggt agccttcatt gccacagaac cgatccggga accgatgctg cggttataca
     2881 gagccatata gcactttacc aggatcgaaa ataccaggat gccgataatg acaggggagt
     2941 agtcaggaac ttccggatgg aaaatcttac ttacagagct ttttaccagt tcgaagccca
     3001 tgagcacgat taaaatagag actaacagcc cggagatata ttcgatccgt ccgtgtccga
     3061 agggatggtc agagtccggc ttctgtcccg ccattttgaa gcctgccagc gtaatgatgg
     3121 aggatgctgc atccgacagg ttgttgaatg catccgccgt gatggcgatg gaatgactga
     3181 aaaagcctgc cagagccttg gtagtgaaca ggatcaggtt gaaacagatc ccgacgatac
     3241 cgcagagcat tccatatgcc tggcgaacgc cggaatccgt gacgttttca tgatccctga
     3301 taaataattt tgccaataat gtaaccataa gatatcccct tttcccaaat gagagtttaa
     3361 gggatattgt agcgcatatg caggaaaaat acaagtaaat ccggatgatt tgcagaaaaa
     3421 tgatttgcag aaaaaacgcc caaagatgca gatcaagaaa tcagcggtta ctattgcgca
     3481 tccgtcgttt tcagtgtata atctcggtgg atgtaatgta agctttcaga acggatccgg
     3541 gacgcagacc attttccgga gccgtactgg taatttataa taatcaaaag taaaaatgga
     3601 ggaacgatcg atgagaaaga aaccggttgt attaatgatc ctggatggtt atggattgaa
     3661 tgataaggta gagggcaacg ccgtagcaca ggccaatacc cctgtaatgg actccctgat
     3721 gaaggagtat ccttttgtac atggtaatgc cagcggtatg gcagtaggtc ttcccgaagg
     3781 tcagatgggt aactctgagg taggtcatat gaatatgggt gccggccgta tcgtatatca
     3841 ggagttgacc cgtatcacca aggagatcca ggacggcgac ttttttaaga atgaagctct
     3901 gttaaaagcc attgataatt gtaagaagaa caacagcgcc ctgcatctga tgggtctgtt
     3961 atccgacggt ggcgtacaca gccacattac ccatctgtac ggcctgctgg agctggcgaa
     4021 gcagcagggc ctggaaaaag tatatgttca ctgcttctta gatggacgtg atacccctcc
     4081 cgccagcggt aagagctatg cagagcagtt aaatgaagaa atgaagaaaa tcggtgtagg
     4141 taagatcgct tctgtaatgg gccgttatta tgctatggat cgtgataaca attatgaccg
     4201 tgtacagctg gcttacgacg ccatgaccga aggtaaaggc ttgaccgctg cctgcggtat
     4261 ctgtggtatc caggagtctt acgacagaga agagaccgat gagtttgtaa aacctactgt
     4321 tgttgtagaa gatggcaagg cagtcggact ggtgcaggac aaggattccg tgatcttctt
     4381 caacttccgt cccgaccgtg caagagagat caccagagcc ttctgtgatg atgacttcaa
     4441 gggatttgac cgtgtgagaa aggatattac ttttgtatgc ttctccgatt acgatcctac
     4501 cattcctaat aaagaagtgg cattccacaa gatcgccatt accaatacct tcggtgagtg
     4561 gctggcagct catgacatga agcaggcacg tatcgccgag actgagaagt atgcacatgt
     4621 aaccttcttc ttcaacggcg gcgtagagca gcccaacgag ggagaggatc gtatcctggt
     4681 aaattctccc aaggatgtgg caacctatga tctgaagccg gagatgagtg ctcctcaggt
     4741 ctgcgggaaa ctgcttgatg cgatccgctc tgaaaaatat gatgtgatcg tgatcaactt
     4801 cgcgaacccc gatatggtag gtcataccgg tgtgatctct gcagccatca aggcgattga
     4861 gaccgtagat gagtgcgtag gcaaggcagt acaggcagtg aaggatgtag acggcgttct
     4921 gttcatctgt gcagaccacg gtaacgcaga gcagatgatt gattacacca ccggtcagcc
     4981 tcataccgca cataccacca atccggttcc gttcatcctg gtaaactacg atcctgctta
     5041 tacgctgaaa gagggcggat gcctggcaga tatcgtacct accctgatca acgtgatggg
     5101 tatggagcag ccggcagaaa tgacaggaaa gtctctgtta attaaaaagt aatcaccgga
     5161 atattttcgg taagatcgca gaaaccataa aagcagagga agattcctca ggcactttgc
     5221 acggtgcctg aggattttgt aactgatttt tacatgatat cttcttatca gcaggaggaa
     5281 gaattatatt atatggaaca gaagaaaaat ctggtaactc tgggcagcac cgggatcacc
     5341 gtagagaaaa actccttcgg agcgctgccc atccagcgga tctccaagga agcagccatt
     5401 ggactgcttc gcaaagccta tgctgcaggc gtgacctttt atgacactgc cagatactat
     5461 actgacagtg aagagaaagt gggcgctgcc tttgagggta tgcgtgacaa aatatatatc
     5521 gctaccaaga ccggagccac cacagcagaa ggcttctgga aggagctgca cacttccctg
     5581 gaaaaattaa aaacagatta tatcgacatc taccagttcc acaatccggc attctgcccg
     5641 aagccgggag atggcacggg attgtatgag gccatgctgg aagccaaaga aaaaggcatg
     5701 atccgtcaca ttggtattac caatcaccgt ctgaatgtag cgcatgaagc cattgaaagc
     5761 ggactgtatg agacactgca gttccctttc tgctatctgg ctaccgacaa ggatctggaa
     5821 ctggtacagg attgcaaaaa ggcaggcatg ggattcattg ccatgaaggc actgtccggc
     5881 ggactgatca ataattccgc agcggcatat gcatatctgg cacagtttga taatgtactt
     5941 cccatctggg gagtccagcg ggagagtgag ctggatgaat ttctgtccta tatagataat
     6001 cctcctgcat taacacccga attacaggcg gtgatcgatc gtgacagaga agaactgcag
     6061 ggagagttct gcagaggctg cggctattgc atgccctgtc cggcagggat cgagatcaac
     6121 aattgtgccc gcatgagcct gatgatccgc agagcacctt cagcagcaca gctgacagac
     6181 gagatgcagg caaaaatgcg taagatcgag gattgcctgc attgtggcag atgtaaatcc
     6241 aagtgtccct atggactgga tactccggca ctacttgaga aaaattataa ggattactgt
     6301 gagatccttg ccggaaaagc gtattaacac atagcagatc tgaataataa cctccttatg
     6361 ggacatactt acctaagaaa aagtaagtta aggatgcctg taaggaggtt ttttatggaa
     6421 aaatatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg atgaatatct
     6541 taaacggcgg aaagcatgcg gcgaacaccg tggatttcca ggagttcatg atcatgcccg
     6601 cgcaggcgga gaactttgcg gaggggttac ggatctgtgc ggaaatttat cataatctga
     6661 aaaaactgct ggaggagaaa ggcttgtcca ccggagtcgg ggatgagggc ggctttgcac
     6721 ccaatctgcc ggatgcggag agcgtgctgg atttcctcgt ggaggcgatc gaggcttccg
     6781 gatatgtacc ggggcaggat atcaagatcg ccatggatgc cgccagcagt gaactatata
     6841 atgaaaaaac gggaatgtac catttcccgg gagagagtaa gctgaaggga gaggaagtcc
     6901 tgcgggatac cggagaaatg atctcgtatt atgaacggct tgtggagaac tatcccatta
     6961 tctctattga ggacggactg gatgaaaatg actgggacgg ttggcaggag ctgacgaaac
     7021 ggctgggaga gaaaatccag ttagtgggag atgacttgtt tgtcacaaat acaaaacggc
     7081 tggatgccgg gatcaaactt cattgtggta atgccattct ggtaaaagtc aatcagatcg
     7141 gaactctgtc cgaagccttc gaagcggtgg aaatggcaca caagaatggt tacaaggctg
     7201 tgatctccca cagatccggc gagacggagg ataccaccat agcagatata gcggtggcac
     7261 tgaatgccgg acagatcaag accggagcac cctgccgcag cgatcgtgtg gcgaagtaca
     7321 accggctgct tgccattgaa gaggagttaa aggatgccgc ggcatatatg gagccgtttt
     7381 gcaaaatctg agtgactgcg caacaaaatg aaaactacaa aaaataaaaa atgtataaaa
     7441 aagaagaatt ttctgcaccg gaaattcttc ttttttttga ggaaaactat agagacatac
     7501 atgggagaaa gaaaaaagaa caaaaatata atgtgtaaat atacatcata tactggaaaa
     7561 tatacactat atacaggaat tgaaaaacag agactatttt taatgaaaaa ctttttcata
     7621 aatatacaga caaatgtgcg tagcacacga acacggtgga gataaatcag gaataagaaa
     7681 aacacggaaa ctggcagatg cttcatatgt aattcagttg acagccttga aaaagtgttg
     7741 taatataaca aaagtcgttg tcggaaaagt aaagtacatt ttacgtacgt gtatacttgc
     7801 atacaaatac gaaaagtgta ttttatgaat gtccgtaggc aaaagaaaag caaggagttg
     7861 aagagatgct taaatatatt gcaaaacgta tcggtctggc gctgatcacg atttttacag
     7921 tagcaacact cacctttttc ctgatgaaca tggtccccgg tggtccgttc ctctcggaga
     7981 aagcagtaag ccctgctgca caggcagccc tggaggcaaa atatggactg gacaagcctt
     8041 tgtcccagca gtatctgacg tatatgaaag atgctttgca cggtgacttc ggagagagtc
     8101 tgaagcagag aggaagaacc gttacttcta ttatagtatc caagttccct gtatctgcaa
     8161 gagtaggtgg actttccgtt atcgtagcgc tgttggtagg tatcccactg ggatgtctgg
     8221 cagctaccca tcgtggcaag acgctggaca gcgtgatctc cgtgatctcc acttgtggta
     8281 ttgcggtacc aagcttcgtt atatgtactc tgcttctgta tttcctggga agtaaggtaa
     8341 agatactgcc aacctacgga atcggcacct ggaagcatta tgtgatgccc gtgatcgcac
     8401 tggcattgta ccccactgct tatattatga gactgatgcg ttcttccatg ctggatgttc
     8461 tgggacagga ttatatgaga accgctacgg cgaagggagt aaaacctttc ttccgtctgt
     8521 tcaagcatgc actgagaaat gcactgctcc ccgttatcac ctatgtaggt cctatgatgg
     8581 cttatactat gacaggaagc ttcgtagtag agaagatttt cacgatcccc ggtctgggca
     8641 atgaatttgt cagctcgatc attaaccgtg actacaccat gatcatgggt accaccattt
     8701 tcctcgcaac actgatggtt gtcatgaatg tgctggtgga tatcgtatat aagctcgtcg
     8761 accccaggat caagttgaag taagattaac aggaaggaag ataccgatga aagaaccgaa
     8821 tgctatgagc tttcatgtaa aatataaacc ggaagatttt atgccggcga acgaagaaga
     8881 gaagcagagc caggtcatca tgcgtgagag cgtgggcttc tggcaggatg gcttccgcag
     8941 acttcgtaag aataaggttg caatggtcag cctcttcgtt attattgtag ttatgatctt
     9001 ttcctttatc gtgccggcat tttatcccta tgattacaag acacagatca aaggaagcga
     9061 gaatctgcac cccatgcagt attccgagaa agagatagag cgtatggatg cgggagagaa
     9121 ggtattcccc catgtgctcg gtaccgataa aatgggccgt gattatgcca tccgtgttat
     9181 gatgggaagc cgtatctccc tgttggttgg tctgatcgct actgccatca ttttcgtgat
     9241 cggttccctg attggttcca tctctgcctt cttcggtgga tgggtggatc tgatcattat
     9301 gcgtattgtg gatattatct atacgatacc ggatttcctg ctgatcgtgc tgctgtcctt
     9361 tgcgttaaag gaccctctgg cgaatctggc acagcttccc ggattcggat ggatgcagat
     9421 cgtaggatct aacctgatca gtatcttcat cgtgttcgca ttgctgtact gggtaggaat
     9481 ggcacgtatg gtacgaagcc agatcctgac cctgaaggag aatgattatg tcatggcagc
     9541 caaagctctg ggagcaaagg atggccgtat cattaaaaag catctgctga ctaactgtat
     9601 cggtacgctg atcgttacta ccacactgca gatcccttcc tctattttta ccgagagcta
     9661 tctgagcttc ttaggcatgg gtgtggcagt tcccctgcct tccctgggaa gccttgcaag
     9721 tgatgccatt aacggaatga tcacattccc gtacctgctg tttgcaccct gtctgctgat
     9781 cagtctgatc atcttaagct ttaacctgtt tggtgacgga cttcgtgacg catttgaccc
     9841 gaagcttaag aattaatgga aggagtagtg aagatggata acaattatct ggtagatata
     9901 cagcatgaac gtctctcttt ctttactcct gccggagaag taaaggcact gaacgatgtc
     9961 tccattcacc tgaaggaagg ggaagtactg ggcatcgtag gagagagtgg ttccggtaaa
    10021 tccgtaaccg catacagtct gatgggactg accgcttatc ccggtaagct gatcggcgga
    10081 gaacttcact tcaatggaca cgaagtggag aaaatgaccg aaaaagattt ttccaaaatg
    10141 cgtggtaaag aggtttccat tattttccag gatcctctga ccagcttaaa tcccgtatat
    10201 accattggta atcagatcat ggaagtaatc cgtctgcata ccgatgcgga taagaaaaag
    10261 gcatatgcaa gagcagtgga gcttttgtct ctggtaggta tcaatgaacc ggagaaacgt
    10321 atgaagcagt atccccacga gctgtccggt ggtatgagac agcgtgtcat gatcgcaatt
    10381 gcgctggcat gtgaaccgaa gcttctgatc gccgatgagc ctactaccgc actggacgtt
    10441 accattcagg cgcagatctt ggagctgatg atggagctga aggacaagct gggcatggcg
    10501 atcatcatga tcacccatga cctgggtatc gtggcgagca tgtgtgaccg tattgcagtc
    10561 atgtatgccg gccgtatcgt ggagaccggt acctgtgacg agatcttcta ccatccgaag
    10621 catgaatata ccaagggcct tctggagagt atcccccgtc tggacagcac gaagcatgag
    10681 aagctgatcc ccatcgaggg taatccggtg gatcttttga atcctcccga gggatgtgca
    10741 ttcgcacccc gctgcaagaa ctgcatgaag atctgcctga gagagaatcc gcccaagacg
    10801 gattttagcg agacgcatta tgcacactgc tttatgaacc agaaagctga gatggaggga
    10861 ggaaatacca atgagtgata agttacttca ggttgaacat ttgaagcaat acttccccgc
    10921 aggcggcatg ggtaaaaacc gtaagttcgt gaaggccgtg gatgatgtca gcttttttgt
    10981 gaataaaggt gagactctcg gactggtagg agagagcggc tgtggtaaga ccaccaccgg
    11041 acgttctatc ttacgattat atgagcccac cggcggcaag atcacatttg acggcaaggt
    11101 gatcctggac gttgaaaata aagtaaaggt agatatgctg ccttaccgtc agaagatgca
    11161 gatggtattc caggatcctt atgcaagtct ggatcccaga atgaccgtag gagatatcgt
    11221 aggggaagcc atagatattc ataagctggc tgccaataag aaggaacgtc atgatatgat
    11281 catttccatg ctggagaagg ttggactgaa ctctgagcat gcgaacagat atcctcatga
    11341 attctccggc ggacagcgtc agcgtgtcgg cattgcgaga gcactggcag tgaacccgca
    11401 gttcatcgtc tgcgatgagc cgatctccgc actggatgta tcaatccagg cacaggtggt
    11461 gaatatgttc gaggagctgc aggagcagat gggactgacc tatctgttca tcgcacatga
    11521 cctctctgtc gtaaaacata tctccgaccg tatcggcgtt atgtacctgg gcaaaatggt
    11581 agagctggca gacagctacg agctggtagc acgaagcctg catccttata ccaagagcct
    11641 gatctctgcg atccccattg cagatcccat caaggcaaga gcaagcaaac gtattgttct
    11701 gcagggagat gtacccagcc ccttaaatcc tcctaccgga tgtcgtttcc gcaccagatg
    11761 tccttatgcg gatgagtgct gtgcacagaa ggaacccgaa tggagagaag tggaaaaggg
    11821 tcactacgtg gcttgccacc atgtggagaa gatcaatgcc tgaaattagg tattgacagc
    11881 tgagcaattt agctgtttaa tataaagcta tacaaagcat agtgcttttg tatgaaaaga
    11941 aagaggagga taactttatg aaaaagaagt tagctatgct gctcaccgca gcaatgctgg
    12001 taggttctct ggcagcatgc ggcagcagcg acaatggttc ttctgcaacc ggcagtgcca
    12061 gtgcaactga cagcaccaag accgatgcag cagcaaccga agttagtacc gaaggcaaac
    12121 agcttaccgt tcagatcggt cccgatcccg agaccattga tcccgcactg aacagtgcag
    12181 tagatggcgg taatatgctg cttcactctt ttgagtgtct tctgacaatt gaccaggatg
    12241 gtaagatcgc tcccggacag gctgagacct gggaagtatc tgaagatggt cttacctgga
    12301 ccttccatct tcgtgatgga ttaaagtggt ctgacggcag tgatctgacc gcagacgatt
    12361 tcgtatacag ctggaagcgt gtatgcgatc ccgtagtagc agctccttat gctgagactg
    12421 ttctgggtat ggttaagggc tttgacgagg cacaggcagg tgatctggat gctctggcag
    12481 tatccgctcc cgatgctaag accttcgttg tagagctttc caatccctgc ccttacttcg
    12541 agtctctggc agcattcgct accctgagcc ctgttcagca ggcaaccgta gaggctaacg
    12601 gcgatgcatg ggctaccgca gctgagacct atatctccaa cggacctttc tacattaccg
    12661 agtgggtacc cggttctcac attactatgt ccaagaaccc taactactgg aatgcagatg
    12721 cgatcaagct tggcagcatc aaattcgtac tgatggagga ttccaacgca gcttatactg
    12781 cataccagtc cggtgatgta ctgttcatca aggatgttcc tacccaggag atccccagct
    12841 tacagggtaa ccccgagttc catgtagagc ctaacctggg aacttactat ctgtccatga
    12901 acctggaaga tcctgcattc gcagatgtaa atgttcgtaa ggctctgagc ctggcaattg
    12961 atcgtgatta tgtagcaaat accctgatgc agggaaccta ctctcccgca tacaacctcg
    13021 taggtgaggg ctgggtagat accgacggca gctccttcaa tgcaaatgct aacggtggtc
    13081 agagctatat ctccgaggat ttcgatgcta accttgagga agctaagcag ctcttagctg
    13141 atgccggcta ccctaacggt gaaggacttc ctaccatcac ctatactacc aacgatgcag
    13201 gttaccacaa ggttgttgct gagtacttac agcaggcatg gggcgagctt ggtattaacg
    13261 tagaagtaaa tatcgttgag tggtccagct tcactccgat gagacgtaac ggtgagtaca
    13321 tggtagcacg taacggatgg gtaggagact acagcgatcc ttccaatatg ttagacctgt
    13381 tctgcaccgg caacggtaac aacgatggta agttctccaa tgctgacttc gatgcagcta
    13441 tggagaaggc agcttccacc atggataccg ctgagcgttc cgcagctctt catcaggctg
    13501 aggatgttct gatggagcag gtaggatgca tccctgttgc ttactacaat gacttctggc
    13561 tgcagagcga gaagatcacc ggtatctggc attccgctta tggtttctgg tacttcatgt
    13621 atggtgacat cgcagagtaa gctgcggatc tgatcattca gaataaaaaa caaaggatcc
    13681 ccgtgagatc gtaaagattt ccgggggttc ttttttgtgc cgccttttcg gtacagtctt
    13741 gtaaaatgtt gttgcaattg tgtacaatat atggtaatat aaaatctgct tgacattagg
    13801 aggtgtgaaa tggaagcatt aagagtagta atcaacataa tttttatatt ggtatgtgta
    13861 gcactgacag tgttagtatt gatgcaggaa ggtaaatccg caggactggg ttccatcagc
    13921 ggtgcagctg agacctactg gggcagaaat aaaggacgtt ccatggaagg tatgctggtg
    13981 aagatcacga agatccttgc agtattcttc atgttacttg cagtcgtact gaatctgaac
    14041 gtattttaat cattacgaag gatgacactc ttgtgtgcat ggcatacaag
//
LOCUS       DAWEJW010000075        14085 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1569_length_14085_cov_56.950820, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000075 DAWEJW010000000
VERSION     DAWEJW010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14085)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14085)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14085
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1569_length_14085_cov_56.950820"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3432 a         3710 c         2859 g         4084 t
ORIGIN      
        1 tcattcatct tagccagctt ggctgcctgg tcggctgcga tgcaggcatc gatgatctct
       61 tcgatatctc cgttcatgat cttgtctaac ttatacaggg tcagaccaat acgatggtcg
      121 gtgacacgac cctgcgggaa gttgtaggta cggatcttct cagaacggtc tccggtaccg
      181 atctggctgc ggcgcatctc tgcttccgca tcgtgtctct gctgctgctc gatgtcatac
      241 aacttggact ttaacagtgc gaatgccttc gccttgttct ggatctgaga cttctctgtc
      301 tggctgtata ccacgattcc ggaaggatag tgggtcagac gtactgcgga gtcggtggtg
      361 ttgacacact gaccaccgtt accggatgct ctcatgacat caatacggat atccttgtcc
      421 tcgatgacga tatcaatatc atcgtccacc tccggcataa ctgccacact gatggtggag
      481 gtatggatac gtccgccgct ctcggtctcc ggcacacgct gtacacgatg tacgccgctc
      541 tcatacttca tcttggaata agcgccctgt ccactgatag tgaactgtac ttccttcata
      601 ccaccgatac cgatctcatc cacgttgatc agctctacct tccaacgctg cttctcggca
      661 taatgcacat acatacgata gatctccgct gcaaacagtg cagcctcgtc accgccggca
      721 ccggcacgga tctccacgat tacattcttg tcatcattag gatccttggg cagtaacagg
      781 atcttcagtt cctgctccag ctcttccacg cgcttcttgc tgtcgtttaa cgtctccttg
      841 atcatctcgc gcatttcttc gtctttttcc tcttccagca tggaaagaga atcttcaata
      901 tcctgcttgc actttttgta ctctgtatac gcatccacga taggtgtcag atcactctgc
      961 tccttcatca gcttgcggaa acgttcctgg ttattcgtga ccgtgggttc atgcagttcg
     1021 cccatgatct cttcaaaacg aatcaataaa tcttctaact tgtcaaacat aactaattcc
     1081 tctcaatata agttgtaaca gttcagccat gaacatattg gagagcaaaa atcatgcttg
     1141 catgattttg tgaacgaata tgttcatgaa ctaagcgcca ggtttatgag caaaggtagt
     1201 cacgaagtga cggaaagcgt gtcagcgcga ctttgcgaat ggtgcgggct gaactgttac
     1261 atacgttcca aaaactaccc ggtccagacc ggcataatcc tgcaccactt gtacctcgcg
     1321 gtacccttcg gtgcgcatca gttccgatac agcctgtccc tggtcatatc cgatctcaaa
     1381 aaagagcatt ccactgctca ccagatgttt tcctgcttct tcgatgatac ggcggtaaaa
     1441 atgtaaacca tcctccgtac cgtccagtgc catcctgggt tcatgatccc gtacctccgg
     1501 catcagcttc tccacttccg cactggcgat ataaggcgga ttcgacacga tgatctcgaa
     1561 ttttccctcc accttgtcaa acaggtcact ctgtaaaaaa tgaacccgat ctgcgttcgc
     1621 cgcatccaaa agcgtctccg cattttgctc tgccacttcc agcgcatctg cagacaaatc
     1681 cacacccagt ccttcacagg cattgctgta atgcagaagg cttaacagaa tacatccgga
     1741 tcccgtacac atgtcaagca cccgcattcc gtcatggagt tccttcaata cttcctccac
     1801 cagaatctcc gtatcctgtc tcgggatcag tacgtgctca ttgacagcaa aggtcagtcc
     1861 cataaagtcc tgctccccgg tcagctgctg cagcggaatg tgctccgccc gctgtccgat
     1921 ccagccaaga tatttctctt ccgcttccgg tgtcaccaac tgttccccgt gcaccagcag
     1981 atcattacga ttcgtaccgc ataccgcttc cagcagaagt ctcgcatcca gtgtggcttc
     2041 ttcgattccc gctgtctgca aagccagaca gcctttttca tagcattccc gataggtcat
     2101 cttaatcctc tggttctgcg gatgcatcgt ccttcatcca gctctcatcc acttcgtaac
     2161 cgaaggtatc ctgtaaatat ttcttccagt cgaacacagc ttctacggaa gcgatggcta
     2221 cctctgccat gctctcatcg ggttccctgg tggtcatacg ctggatccac ataccgggag
     2281 cagacaggat cttaataaac acattatccg tgcgtccggc cagacggatg atctcgtagg
     2341 aaattccggc cacaaccggc atcagcagaa tacgaaggat cactttctcc gccacattat
     2401 ccacctggat aaagaagaac agcacaatac ttaccagcat aacaaagaaa atgaagctgg
     2461 tgccgcaacg tttgtgcagt ctggagctgc gcatcacatt gtgcaccgta aggggtctgc
     2521 ccttctcgat acagttgatg catttgtgct ccgccccatg atactggtac agcctgcgga
     2581 tatccttcat ggcgctgatg ccccatacat acagcaaaaa cagagcaata cggatcacac
     2641 cctcaataat cgccatcagg gagcggttgc ggataaagct ctcaaacagg gaggaaatga
     2701 aatacggcaa tacgataaag atgcctactg ccaacataat ggagatcacc gtcacaatac
     2761 cggacagcag cttttctccg ttgccaccgc tcatcttgtc cattgcctta tcgaatttcg
     2821 tttctttgcc ttcctcttct tcatagaagg aagcagaata attcaatgct ctggttccca
     2881 ggatcaggga atccaggaaa ttaaagatac cacggataaa gggaagctcc ttgaacttac
     2941 ttccgtgagc cagaccctga taggtctcca cttccacttc aatctcgccg tccggcttgc
     3001 ggaccgctac cgcatacttc tctttatttt tcatcataac gccttccagc acggcctgtc
     3061 cgccgatacc ggaataacga ctctttcttc tagccataaa acctcatcct tataaatgca
     3121 gtattaaata ggaacacagt ttcctatacg acaagaaaag gttgagataa gaatacctca
     3181 acctttcgct tgcaatctta tttaacaccg tatttcttat tgaacttatc aatacggcca
     3241 tcggttcttg cagacttctg ctggccagtg tagaatgaat gacacttgga gcaaacttct
     3301 acgtgaagct cgggcttggt ggagccggtt acaaatgtgt taccacagtt gcaggttaca
     3361 gttgcctggt agtaagcggg atggattcct tctttcattt ctttcacctc tctataaaaa
     3421 tgtcgtgtat aagttcttgt tctcatcctc actgcttatg gaaccacaaa cagcggtatt
     3481 attgtaccat aagagtttac acgacgcaag acctattttt acatatttct tccctggtat
     3541 tgtaccgtaa tgatataaac tgttttttcg gtctcatcaa tcttataaat tgccatataa
     3601 ttatcgacca gaagctgttt ataccccttt ccggcatact ttccctctaa tctatcctga
     3661 tgtgaaccgg gaaaagtctc cagttttttt atcgctctcc aaattctgtc tgtttgtgct
     3721 tttgcatttt ccggaacctg tttttctatg gcgatatacc tgtagatttc ttccatatca
     3781 cgatatgctc tcggatacaa ctttatatgg tatttaacca taatattttt tgtctagccg
     3841 ttccattgcg acatccatat ccatcgtatc cgcacccgca gcaacttcct cttccgcctc
     3901 acagatggcc tgatcgattt tatgaattcg tgcaaaacgc tcataaagtt ctgcgctcat
     3961 cactaccaga tcgctgtagc cattcttagt aataaaaatc ggttcctgct ttgtatgtgc
     4021 taattcggat atttcacttg tttttctcaa atctctgatt ggcatgatca gtggcataat
     4081 agcacctcct ttatgccata attgtagcgc aattatggca catcgtaaag gtatatattc
     4141 tgccatcatt tccaattttt gtcaattttc tatctcttgc ctagataaac cggttcttct
     4201 tcaccatatt cacaaattcc acattattgc gggtatggga gaacatatca aggatgcggt
     4261 cggtagcttc gtcgggcttt aagccattca gggccttacg catgatattg atggcttcca
     4321 gttcttccgg tgtcagcaac agatcctctc ttctggtgcc ggattttgcc agatcgatag
     4381 ccgggaaaat acgtttctcg gacagctttc tgtccagaac catttccata ttaccggttc
     4441 ccttgaattc ctcgtatacc acatcgtcca tcttgcttcc ggtctctacc agtgcggtgg
     4501 ccagaatggt caggcttcct ccctcacgca tattacgggc tgcaccgaag aatctcttgg
     4561 gcatgtggag tgcggcggga tcgagaccac cggacagagt acgtccgctg gggggaacca
     4621 ccaggttgta cgctctggtc agacgggtga tactgtcaag caggatcacg acatccttct
     4681 tatgttctac gagacgcttt gcacgctcga tcaccatctc tgatactctc ttgtggtgct
     4741 cgggaagctc atcgaaggtg gaatagatca cttccacatt gtctccgtgg attgcttcct
     4801 taatatcggt aacttcctcg ggacgctcat cgatcagcag gatcagcatg tgcatcttgg
     4861 ggttaccgtt caaaatagac tttgccactt ccttcaacag tgtggtctta ccagccttgg
     4921 gcggggatac gatcatacca cgctgtccct taccgacagg gcttaccaga tccatgactc
     4981 tcatagccac gctgcagccg ggagtctcca tcttgatgcg ctcatccggg aagatcggcg
     5041 tcatatcctc gaatgccatt ctcttggctg actcttccac tgtataaccg ttgatgctct
     5101 tgacgaacag cagtgcactg aacttctcct gctgggtctt gattctcttg ttgcccttca
     5161 ggatatcacc ggtcttcagt ccgaaacggc ggatctggct gggagccaca tacacatcgt
     5221 tctcaccggg catataattc tcgcaacgga taaatccgta accatccggc ataacctcta
     5281 agataccgct ggcttcgata ccgctgtcca gttccttggg atactggctc tcatcgtatg
     5341 ccacgttatt cggattgaag gtacgctctc tggtgtaagc ttcacttctt acgccgacca
     5401 tctctgtacc ggtttcggaa cggtcagtgc gttctgcacg atccgtacgc tcggtacggt
     5461 ctgtccgata gtttctgtca tttctatcct gtctatcata tctgtcctgt ctgtaattcc
     5521 tctcctgtct gacaggacgt tctgttctct ccggcttctc tgcctcttcg ctcttagcag
     5581 cctcctgttt ctcttccgct gccttagctt cttcctcttt tttcaggcgc tcatcttctg
     5641 caagcatagc ttctaccaga tctgcctttt tcattgtgga agttcccttc aggccacgta
     5701 cttttgccag ctcctttaac tgaactaatg caagtgattc atatttttct ctcatatacc
     5761 ctcctatgga aataagattt actataaaac tgttaactaa ggggaattac atccgattta
     5821 cattcgacat gtcagttgaa taaaaagata ttaaaactgc atctgaagcc attataatac
     5881 atttctttcc aaataggaag tcccaaaata aaaattttat tttttgtaaa agtggtgcta
     5941 aatccttgcg aagatatcga ttctattata tcattatgaa tagcgtccgg atttcgtatc
     6001 cggcaaatca tccatgatta tatgaagtaa tgaccaaaca aaaaataaga gaggtattgt
     6061 catgacaacc aatgaaaaag cgttactgat gcatgaaaaa tggaacggta aactggagac
     6121 cgtgtccaag actcccgtca agacccgtga ggatctggct attgcttata ctcccggtgt
     6181 tgcggagccc tgcaaagtaa tcgcccagga caaggaagct gcttacaaat acaccatgaa
     6241 ggccaacacc gtagccgttg tctccgacgg aagcgcggtt cttggtcttg gcaacatcgg
     6301 tccctacgca gccatgcccg ttatggaagg caaggcagtc ctcttcaaag aattcggtaa
     6361 tgtaaatgct gtccccatct gtctggacac ccaggatacc gaagagatca tcaaggctgt
     6421 gacctatctg gctcccggct tcggcggcat caatctggaa gacatcagtg ctccccgttg
     6481 tttcgaaatt gaggaacgtc tgaaggagat tctggacatc cccgtattcc atgatgacca
     6541 gcacggtact gccatcgtcg ttctggccgg tgtcatcaac gccctgaagg tagtcggtaa
     6601 gaaaaaggaa gactgccgcg tagtggtaaa cggtgccgga agcgccgggg tggctatcac
     6661 caaactgctt ctgacctacg gtttccccaa tatcatcatg tgtgacaagg taggtattgt
     6721 ctccaaagat accgaaggcc tgaactggat gcagaaaaag atgaccgaag tgactaactt
     6781 aaacaacgag accggctctc tggccgatgc tctgaaaggt gccgatatct tcatcggtgt
     6841 ttccgctccg aacatcgtca ctcccaagat ggtagcttcc atgaaccgcg attccatcct
     6901 gtttgccatg gcgaatccca ttcccgagat catgccggat gtagccaagg ctgccggtgc
     6961 ccgtgtcgta ggaaccggac gcagtgattt tcccaatcag gtgaacaatg tggtcgcttt
     7021 ccccggtatc ttcaagggtg ctctggaagg tcgtgctacc cagatcaccg aagagatgaa
     7081 actggccgct gccgaagcca ttgccggtct ggtacccgca gacaagctct ccgatgacaa
     7141 tatcatgccg gaagcatttg atccccaggt agccgaagtt gtggcaaacg ctgtaaagag
     7201 ccacatccgc taatgtgttc ggacagcatt gtgagagact ggcacggcaa gccctactac
     7261 tctctggatt cctattgcaa aaatacgttt caccataaat gctataaaat agccctgaat
     7321 gcccggatgt cctgtcccaa tcgggacgga actctggaca ctcagggctg tatcttctgt
     7381 agctccggcg gcagcggtga ctttgctgtg gacattgccg ggaagactat ggaggaacag
     7441 attgcggaag gcatggcaaa attcggtgat aaagtgaccg gtcatgacct gatcgcctat
     7501 tttcaggcat acaccaacac ttatgcacct ctcccctatc tggaagaggt gtatcgttct
     7561 gccctgagcc atcccaaagt ctgcggcatc tccattgcca ccaggccgga ttgtctgggg
     7621 ccggaagtac tggctctgct tgcaaaatta aaaggtgaat atccggacaa attcctctgg
     7681 atcgaactgg gattacagac aatccacgaa gagactgccc actatatccg cagaggctat
     7741 cccctgtcct gctttgagaa agcctgtacc aatctgaaga cactcaaaat cccttttatc
     7801 gttcatacga ttctgggact ccccggagag accgaccgac aggtactgga aaccatgaaa
     7861 tacttaaacc atattgctcc ctttggcatc aagctccagt tactgcatat tttaaaaaat
     7921 accgatctgg cagaggatta tgaaaaggga atatttgagg cactgactcc ggaacattat
     7981 ctggatctgt tggtctcctg cctcgcccac ctgtctccgg acatcgtgat ccaccgggtc
     8041 accggtgatg ggccaaaaga tctgctgatc gctccgaaat ggagtctgga caaacgaaag
     8101 gtactgaact cactgcacca ccgtatgaaa gagcagggca tccgtcaggg tgacctttat
     8161 gaagcaataa ataaagataa acattagaaa ggctgttatc ccattatgtt acaagatccg
     8221 cttactttat ataaacttat cgttctctac atgctgaacc gtgtgacttt ccctctcacg
     8281 gccgcacagg tcagcgattt tatccttgga aaggaataca cgaacttcct gaccctgcag
     8341 caggtcatca atgaactgac ggatgccgga atgatcgcca cccagtcgat ccgcaaccgg
     8401 acccacctgt ccatcactgc cgagggcaag gaaactctga atttcttcga gaaccgcatc
     8461 ggtgatacca tcaagcagga aattgacagc tattttcagg aaaacgagtt caccctgcgc
     8521 aacgaagtct ctgtgctggg ggattattat aaatccacct ccggtgaata cgaggctcat
     8581 ctggtagcaa aagaccgcgg tatcaatctg gtggacatca cattatccgt tcccgtggaa
     8641 gaaactgcgg ctgctatctg cgacaactgg cagaaaaaaa atcaggaaat atacaaatac
     8701 ctgattacgg agttgttcta atcccgcaat caggtacctg attttatgat tctttgtttt
     8761 tagaagaagc ctctgcggct tcttttttga ttctcgccat atgctctctg atcttcacct
     8821 cataaccgtt acccgagggc tgatagaact ccctgccact taattcatag ggcagatact
     8881 gctgttccac atagtcattc gggtaatcat gtgcatattt atatcctgtt ccgtgtccca
     8941 attttgctgc tcctttataa tgtgcatcct gcaaatgtgc ggggatcggc agatctcctg
     9001 tggcctgtac ttcctgcatc gcctgaaata ccgcttcgca gctacgattg ctcttgggtg
     9061 ctgtagcaat ataagtcact gcctgcgcca gaatgatctg cgcctccggc atgcctaccc
     9121 gctccaccgc cagagaagca ttcactgcca tcaccagtgc ctggggatcc gcattgccca
     9181 catcctccga tgcacagatc ataatacgtc ttgcaataaa agtaacgctt tcgccagcat
     9241 ttaacatcct tgccagataa tatactgccg catccggatc tgacccccgc atactcttga
     9301 taaatgcaga aatcgtatcg tagtggttgt cactgttttt atcgtatctc gccgctctct
     9361 tctggataca ctgctgtgcc acgtcgagcg taatatggat cttgccatcc ggtcccggtt
     9421 ctgtggtcat gatgcccagt tcaatagcat tcagtgcatg tctggcatcg ccgccggaaa
     9481 tatctgccag aaaatccagt gcatcctcgt ctatggtgcc gttgtaggct cccattcccc
     9541 ggtctgtatc gtagactgct tttttcaaaa gctctttgat ggcttccctc ggaatcggct
     9601 tcaattcaaa gatcaccgag cgggagatca gagcgccgtt gacttcaaaa tagggattct
     9661 ctgtggtagc tcctatgaga atgatcgtac cgtcctccac atacgggagc agataatcct
     9721 gttgtccttt attgaaacgg tggatctcgt caatgaacag aattgtacgc ttcctgtaca
     9781 ttccctgcag ttccttcgcc tttgccacca cttcttccat atcctttttg cccgccactg
     9841 tggcattaat ctgttgaaat tcagcactgg tagtattggc aatgactttc gccagcgtcg
     9901 ttttgccggt accgggcggt ccatagaaaa tgatggagct gatcttgtcc gccttgatgg
     9961 cacgatacag aagcttgtcc ttgccgataa tatgctcctg tcccacgacc tcatccaagg
    10021 ttctgggacg cattctggcc gccagtggag cttccttttc catattggtg cttctcatgt
    10081 attcaaacaa atccataata ttacatcctc tgtttctatt gcaaagatac caaaagactg
    10141 aagcatattc taagatatgc tccagtcaat tataacaaag tttatcgcag cttgcaattt
    10201 tgcatccaga tcttcacacg ccgcttcaat gcatttgctt ccagcggagc cagcaaaaga
    10261 tcctggatta cttccgtctc caacgcacat ttcatctgta caagattccc ctccttgcag
    10321 atggcgatca tgggaagcgc atgttctctc tgtaattctc caagttcctg ccagcgtgcc
    10381 tgtcctccgt cctcttcttc tgatacatcc acgaacagtc ccagaatgtc ttcgccggtc
    10441 ttctctccca tgttctctct gatctcttcc accgagatta ctttcttcag atgaacaaac
    10501 tccggcagtg caaactccag catgctggta acattacggg aggatgacca cacaaaagcc
    10561 tgtaatttct gatcaggggt ctgattttcc tcaccgaaca cgctgtagcg tccttttccc
    10621 gacttcttgg atacgtacaa tgcctgatct gcttttgccg caagatcata gtagcaatga
    10681 tcctctggat cggaaaatgc tattcccaca ctgatggata tattttccgg tatggataag
    10741 ttctctttat attttacgag gttgaccaga ttctctgcct tgatcagtgc gatctctttg
    10801 gacggaatgt ctcccatcaa tactgcgaac tcatctccac ccatacgtcc cacataatct
    10861 gtgctgcgga actgtgaaga aatgactcct gcaatgacgc tgatcacatg gtctcccata
    10921 gtatgaccgt atacatcatt tacggatttg aaattatcga tatctatcac cataagagca
    10981 tgcaggccat tctcatgctc tgtcagagtc ttcgtgatca gcttctctac tgtcatcttg
    11041 ttgaacagac tggtcatctc gtcgatctgc gactttgtct tccaggcctt ctgctcatct
    11101 ctgctattct gcaacctgcg ttttaccccc agtgtattgt tgatgcgggt aagcgctatc
    11161 cgggtatcta ccggtttgat aatgaattca tccgccccca gtgcaaatgc ttccgactgg
    11221 gaagacaccg attccgtatt cgtcagaacg ataaccggcg tgtccttgaa gccctccata
    11281 ttctgtctgc ggcgcagtac ttcaaagccg tcgatccccg gcatgaccag atccagcagc
    11341 accagatcaa tggcatcgct ttccttctcc atgatcgtaa aacattcttc accggaaaca
    11401 gcctctgcga agttaaaatg ctccccgaga gactgtctga tcactactct ttgaaatttt
    11461 gaatcatcaa cgattaataa tgtttctttc ttttgtaaac tgctcatggc tgtcggatcc
    11521 tgctttccgt atcacgtcgt ttaaaacgcg atacagttat agtatatcca acagccatgt
    11581 gctcgtcaat ttaaaaatat atctactttt tacacaatct ttatgtggtt tttcccttta
    11641 aagttgaatt ttactacgtc tgcagagaag tccgccgaat gtcaccagac ctgcagctgc
    11701 aaacagcagt acctcgggca tccatcccat cacaccttca ccggtcttag gcacgctgtc
    11761 cagctccggc tgtgcagctg cggtgtttgc agctaccgta aatgtggtgc tggcggaatc
    11821 atcgatccac atggtgtcca cagtatgttc accctctgac agggatgcca ggtaggaagg
    11881 cttcagggtc acgatagtgg aacctgattt tgcttcgtag tgggcactgg ggatccagtt
    11941 gccgtctaca cggacaccgg cgaatttagc aaaatcacca tcgccgcgga tggtcaggcc
    12001 ttccgtggtg ctgccgttcc aggtaccctt ggtgccttcg atgatcttat agggatcttc
    12061 ctggctgtca tcggtaccac tgtcagaagc tgatgtatct gtattatctg tagtatttgt
    12121 aatctctccc gttgtaaagg tatatttcaa tgtcagatac cgtccctcag ctaagtcaat
    12181 tgacgtatac tccacttgtg gatttgtgac aatccagatg ctgtcatgat tgaatttata
    12241 gccacgttct gcaaccaagg tcagaattgc ataatacgtt gtgttcggtt cagcattgcc
    12301 gattgccgcc tcattctcat ttccctcttt caaccacaaa atactgttct tcgtaacatc
    12361 agtaattcct tccgtattaa tctgccattt tttaggtaag ggctgtccgg ctaccggtgg
    12421 ggtgactacc atgtccagac tggagattgt ccgatcaact gtgccaacat taaacgacat
    12481 tatatctgtt ttcctatgtt catccccttg tggatcatca tatactgctt ttgcatacac
    12541 cttatatccc gccactttgg cacagttttc tgttttatag ctaatcttca ctgttgcagt
    12601 tctgttatct tcactataat ttatgatttt tgcatcaaaa atgtcatctg cacggtaacc
    12661 cgctccatca tagaaattaa atgaaacatt tactttagaa aaatcataat atgaatttat
    12721 ttttaaatca tacaaaaaat aagatttctc atcatttgaa accaactgtg ccggttccga
    12781 caagctagaa tgaaattcca gattgactgc ttcacctgtc ataactattt tacctgttcc
    12841 atgatttgga agccgataat aataacattg ttgaacgctg tcataggttg cacttgcttc
    12901 tatttcatta acatcatccg tagaatatac aactttcggt gctgtcaaaa accaatattc
    12961 ttttccatta taactgtcat ctcccgggta tatagaaaaa caatcataca ttttactatc
    13021 tatcatagta cttagcatta tatgacagtt ttttaatccg gttctatcaa ctgtccagtt
    13081 tcccgtataa tcgtccgcct tagcattcat cccctgccaa ccccacagca gcagcaccgc
    13141 cgctgccagc agtccgattc tctgtaatat ctttttcatc tgtttcctca gagggatatt
    13201 gtcaagtgtg taagtaagat tttatgaaat tgcttctatt ttcataagag agccttccat
    13261 tacttttata ccatctgtta attcaatata gctgattacc tttgtttctc aaaaagaata
    13321 gtttatacgc tttagatttt agtatcaagt gttgcctgtg caggggattt tcctgtctct
    13381 gcagtctctc ttgtaacatc atcaagtgct ccagctttga ttggcttacc aatttcccag
    13441 ttaggatcag ccttttttgc ggcatcataa aaagcctgtc tgtaaattct ctcatatttt
    13501 tcaagggtgt acccggcagc cagcctgtcc tttttggaca ttttgcgata caaattgtta
    13561 tacacatctg ttcgttttgt tgtatcacca ttgcatacac cattttcacg cagaaattct
    13621 ttttttgtca gttcatacat ttcttctttg ctgctatccg gaattgatat gatccttctt
    13681 ctcgaattct cgttgtcatc tgtcaccaaa agtcctgcca atccatttac aggattaata
    13741 taatctccat ccttatcata atgactcatc agatttttta ttccctgaat attgccatac
    13801 atcgcaccat ttccggcaga catcatcttc ttaatagctg ctttatactg tttactgttc
    13861 gtattaattc cagcagcttt tagctgtgat tgaacagaac tgctattaag ctgtgacaac
    13921 tgaaaactac ctattgcatt tactcctgat gttccaaatg atttctgaaa taaaaaagca
    13981 taatccgtta tccttgacat aaaacagcct ccctgtctgt ttccacattg caaatttatt
    14041 tttgtctatt ttatgttcta gcataagtca ttattttaat tgcac
//
LOCUS       DAWEJW010000076        13909 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1588_length_13909_cov_48.685506, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000076 DAWEJW010000000
VERSION     DAWEJW010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13909)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13909)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13909
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_13909_cov_48.685506"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3642 a         3011 c         3266 g         3990 t
ORIGIN      
        1 ttattattga tcaagtacac actttttgaa ccgaaaacaa gaaaaacgct tctggagtgc
       61 tgaccaaaag cgttttgtct acagtctgaa atccctgttt cctattaagg agacagggat
      121 ttatataatt tagataatac tgggatcaaa gcggtaagat tgtaaacgct atggagcgtt
      181 tacgtaagat tcgataagct tctcaaatat tctgctttac aattctggaa atcagcccaa
      241 ccgccacatt ccgttccgaa tcaatccggg tcaccacgaa gctgacttca tctttttttc
      301 cggggagtct tgagtcatag cagctgtggg caatcgcatt gactccaata ttaagtctta
      361 taaagacggt gcccttgtga atgtctgtaa tggtaccgat atatttgccc tgaaccttac
      421 atttctgcag attttctaca ctggtattgc caaggacact tttaacatcc gcttttacag
      481 aaatgtcagt aacagaggat aaggaaatgc ttgtgatacg gactaagatc tggtcaccta
      541 catggtagtt ttctgtggca tcccccatcc agtcgtagga gagatcccta gcagggattg
      601 agtattccac accaaagatc tcagtacgga cagttttttc ggcgacagca atgactcttg
      661 cctgcacgat gcgcccttca cagactcttg aggtgccatt tgcatccggc aggtagaaaa
      721 tctggcgttt cttaagcatg gcatctttcc tgctagcagc aacggagcgg gatttattgt
      781 cgattccctt gatcagaaag tcgatttcag ctccgagcat attgccgagg atcttatttt
      841 gtctgttaag gagctccccg tagtcggaag tggcatcttc ttttaagttt atcatcattt
      901 cggataaggg aattacaata cgctggtcct tatagtagac aatcgctatt gttccatcgt
      961 tctccagttt ttcgataccg ccgagtgtgc cggtgaggat ttttttggtc cggtaggcgt
     1021 tttgcagctc atgccagttg gtatcttcaa tggactccgg cgtttcgatg tctgcgtctg
     1081 cagagattgt aaggatagat gtgggggcaa gtttttcagg ggatgcgggc aggatagttt
     1141 ttttttcttt ggttgtaatt acctgttctt cagttttctt aggcataata ggttctcctt
     1201 ttctttactt tgacatgatg gatgatttat ccgccatgac gatttctggt tgagttttcg
     1261 ttgatgtctt ctttttagta ctctgtatgg gtgcagacca ttcttctttg ttttgcactc
     1321 tccatgccgg gatatggtct gtggcttttg tgtactgaag ttttttgctt tccggatgca
     1381 gtgaaaaatc gtatttgccg acctttagaa tcttttgtcc acgtaagata ataagagcct
     1441 gatctaaggg aaggcgtaga acctcgtccg gggttaaaag tttccgcttc ccaacggacg
     1501 ctgtttcttt atactgcggg gaataatccg taatacgtag ggtattaagc tgcttggact
     1561 tgctggatac atctacactg acatctccgg atctatcgga gataaattcg gcggtgagct
     1621 gatcggtaca gcctaagaat agctgtgtat cacagttacc aataatttcc tgccactggt
     1681 tatccggata tctgttctgc atctggggca gattttggaa aatacaactg atggataatc
     1741 gcctggaacg gatcgtgctg atttttttgg tgagatctgc aatggtacag ccaccgtttg
     1801 caatctcatc tgccagaata tgcaccggca ccgggagctt acagccgtcc tgctcgtccg
     1861 cataacggac taatttgata aaaacaaagc tcataaacag gctggataag aagtcaaagg
     1921 tcgaatcctg atcggaagtg atgcagaaat aagcacattt ctctttgccg gggagcgtta
     1981 agtcaatctc gttatgaccg gtgatctgac ggatcagttc attctggaag acctgaagtc
     2041 ttattcccag accgcttacc acatcatttc tgacactttc ctgggctctt ctaaagatgg
     2101 agtacgggta ttttgccgga tgagtgtttg ggagcatctg aaaaagactg cagagttcct
     2161 cttcggtatt taaggtaagc agcttatata cctgtccgat gttcctgcct tccgggggaa
     2221 atccataata tacatacaac acggccgctt tcaggatatt cagttctgca tccgcccaga
     2281 acctggtttc atttttagaa gaggtgttct ttatgatgac atcgcagaat aactgtgcca
     2341 tggtatcctg accatcaatc tcggataagc agttccaact gtcggaattt tccgggtgaa
     2401 tcaggttaaa tacccgcacc gtatagccat gctgctccaa gtaaagagca aaatcaccgt
     2461 ataattcaga cttgggatcc gtgacaatga gactttcccc acgggcaacg cactgaaaga
     2521 tcacattcct tgcgtaggca cggctcttca tggagccact ggccccaaag acagcaacat
     2581 tgcggttata gggcgaactt tcagggagac atacgatttt gttttccagg gtccctaaaa
     2641 taatgccggt gctacggcgg atatcggaag tgagatttag gacattgcga aattcatccg
     2701 gtgtcatgta gccgctggtc ccataagtcc ccttctcaga ataggaaaaa ttccgctcct
     2761 tatccagggt cccttccccc ccgattccca tgcgcatcac cacgatgatg agcaggatag
     2821 caataagtag gcataaaaaa atcccataca gactgtacgg gaagatggta agtgcctgca
     2881 gacaggagaa aaaggaagca tccggaaggg gaggagagga accgtctccc ggtgtacctc
     2941 cggcagatat ccaggtgttg tagtgataga gaaactgagc gatgtatcct gcggcataga
     3001 gcagtgtggt tattaacacc ggcaggacaa ggagcacaca aagcagcttc tttcgttttt
     3061 ctgataactt cagtggaaaa acctcctttc aaatttggca agatcaatgg tttcaatggt
     3121 tgcaaggtcc ccaaaataag actgcaaagt atctttttgg aagtcgaagc agatgaggtg
     3181 cccggtaatc tcgtgaaaag acagcgcggt cttaaatctg gagagccgga gcatatcaaa
     3241 attgtaggat aagagaaccg gagtggttcc ctttatggca tcatggatga gcccaagatc
     3301 cggatttaca ggttccagat ctgaacgaag cagggaatcc agagtgctct gaagagcagg
     3361 gctgcataac aatttcagta gagtgatgcc ttccatggag actggtagaa agtgcatata
     3421 gtcaaaactc ccatcgaggt aaaagtagct tttctggaat ccaccattac tttccatgag
     3481 cgttctggca ctatccatgg gacttccaag aaagagtgct ttgataggat aatctatatt
     3541 ctttcgacat aaccccggaa acatatcctg cttcaggtga tagtttagga aagcttttag
     3601 tctaagctcc gtccggtatt cccatttgat cgggtggtcg ccggtaaagt agagaacata
     3661 cagggcatcc tcgcataaca ggattcccag agatctggaa ttgttgattt tgatcgtttc
     3721 ggcccccagc tctttccatt ccctggctgt gtaaaagagc gggaacgcaa atttggcaga
     3781 atcttgcggg ttcccggaaa atagagccgg cttttcctct ggatacacag ggattcctgc
     3841 gcattgcagc atggcatagg cgatagaagc ctgctgaagt ctgagtctcc ttggcaggtc
     3901 actttttggg cggttggttt cactgttgtc cgtaagataa taggaaaacc gttcagggga
     3961 cagggagagt agtagacctt ttccgcggcg ggttaatcgg atcccgcgta gcttgtcctt
     4021 ataatgaatc ttgataagac cgtcattctt taatctggtc atcagcttat cgttgtagct
     4081 gtccgttagg tataaaaaag ggtatacatc tttactgcac tccccggata tggcgatcag
     4141 ggacagcagt ttatacgctt ttgatgaagt gtccatggag ttctcctttt tgtagttgta
     4201 gtgcgcagat tttaccatac cagactgaaa ctccggtaaa accggaagtg ggttaggaac
     4261 aagaaagtcc acaacagagc ggatgcgcgg ccagcttatc tgtatcggat ttttacaggt
     4321 ggaaaagaac taaaaatccg acaggttatt ttgcgcattt ttctgctcca aaagccattc
     4381 cgggaactgt tctttttcca tgatggttag aatggtagca tctttattgc caaattctgt
     4441 tctatggaaa gtatcacaaa aaataccgct gtcatcggta agtaagaaac aggcgacagt
     4501 atccagtatc cgcttgcatt cctgggaatc cagattgtca tagtcccgta ctctttttgc
     4561 ggacagtgag gcatcataga tatgcaggaa acttactaca cagtccttaa aatgtggaag
     4621 aggatgctcc tttacataaa gctcaaacgc agtatataaa ggatccgtca ggtacgctgt
     4681 gttagaatac ttagcttttt ttggcatgag ccccggaagc gtgacctgaa cgatcccact
     4741 agagtgtgta atgatgattc cctggatgtc tgctgctttt tttagaagca tcggtttggg
     4801 caccaggttt gctgcaaaga taagacttcg taaggaacag gtgatttttt ctgctcgctg
     4861 cgcagcatcc aggctgagtt cctcatagat ttttccgtaa gatacggtat ctgtgatctt
     4921 catggcattc agggtgtttg ccagttttgg cagttcctgt tcaatggaag caagtttggt
     4981 taaataagtc ggtcgatcca tgaaataatt actccttaga ggtataatag gtaatctggc
     5041 cctctcccga taccgtgcag tatccaaccg taccgggaat ggacagtaga gatatttgtg
     5101 ttacattttc tacgacaacg atgcgcttag ggtatccctc tttgtagaaa gaaagtgcct
     5161 ggttaaggat ctgttccttt tccaagggaa tgaatatcac atcgaaacaa tcatccgtgg
     5221 cagtaaaact aagcgttacc ggatagtcag aaactgtatg gaagttgact tccgggtaaa
     5281 aatccaaaag aacccagaag gaagagagaa tggcagggtt tgcttcagtg ataccggcat
     5341 aatagaccag gtcctttttg gaatccagaa cgatccgacc ctttcgcaca agcttcttta
     5401 acagaagggt atgctgtgtt atggaaagct ccgggaaaat gcggcatagc tgcgaaaaat
     5461 gcagacagcg gtacatggca agcagactgt taagttttga aagaatattt tcttcctgct
     5521 gatatatctg atgcatggtc tctcctttgt gtttatacgt attctgtatg tacgaaatac
     5581 gtattattgt gattgtgttt ctacatatca aatatgtaat atatacgtat taaccggcat
     5641 cgggtaaatg gtgccgttgt ttctcccgtt ccaacagccg ttttaactct tctctgtcgg
     5701 tggtgatcag ctctttttcc aattcggaag ccttgaactc cacggtcacg ttattgttgt
     5761 tggtagagat caggccgttg ccgcgttcaa aatgggtgat cgccatggtt tctgcttctg
     5821 acaggtgcag gatattctgc acacggatgg cctcctcatc ttccaaattt aagatgatct
     5881 tggtcttgga gttattgatg attccttttc cgtatttgcc ctcttccatg gaaaagaagt
     5941 cgtttagatc ctgggtggca cagatcgcgg atccgccgta gccacggacg attttgaaaa
     6001 tctcaacgac aaattctgcc gcgaggtggt tggacgcaga accgataagc tgccatacct
     6061 catcaataat gatggctttt tccgcagtac ggtcttcttt ggctttatcc cagacataat
     6121 ctaaggcaac gaacatcccg accgtaagca gatccccggt aagttctgag atatccagta
     6181 cggtatattt gttttcgagg tttacgttgg tctgctggtt aaaggtgaag gcagatccat
     6241 gcacaagcct gttcataata tttgcaagtc ttctggtatc ggcattctcg atcaggatat
     6301 tgtagacatc ctcaagtacc ggcatttcct tgtaatgcga aggatctttg ggatcgcaga
     6361 gactgtcatt attgtgagtg atgccctttt tgttataggt ctggatcagg gattcatcca
     6421 aaagctgtct ttcctcatgg ctcatatccg gaatcaggag cgagaagaaa atatggagct
     6481 tctggatctt ggctgcaagt aaggacttat cctgggaaga accgtcaatc agatcatttg
     6541 cagaggaatc aatttttcgg atttccatga tatttataca gtttttggag gcagggctga
     6601 tctggataaa ttctccacca atgttgttgc aggccctgcg aaactcatgt cctttcaggg
     6661 gagcgatgat gaatacctgg atattttttc ttcgcatccg aagtgccatg agctgtaagg
     6721 taaaggtttt cccggctccg gaggtaccaa ggattgccat atttgcattt ttgtagatcc
     6781 gtgagttgaa aatgtccaca ataataaggg aattattgtg tttgttcacc cccagtaaga
     6841 tcccattgtc atcgcacatt tcatacgaag tgaagggata gcagctggca attccgcttg
     6901 ttagtacatt acgctttgat ttttcaaaca gacttttttc aagacggaca atggggagag
     6961 aggacaaaaa agcctgttcc tgacggaaac tgcaggtaag aacatccata tcctgggaca
     7021 gcagtaactt tttcatctct gcaatgcgcc attccagttc ctccacactt tctccggtaa
     7081 tggtgattaa gatgttcagg taataaaagt cctcgttggc aagaccttcc tttagaaagt
     7141 atcctgagcg gatggcgcta tccagatcat caaaatcaga gttggtgtcg gaagtatcct
     7201 ttattttgga acggttgata cgaagctgct gtcctaactt ttgctggatc ttatctttag
     7261 gctgtctgtc aaagaaaagg tctacatcta ttccttctcc ggcattgaca aggagggaaa
     7321 tccatccggc gcacacctga gcccggtacc cgaaacttgg gatcaggaga taagcgtggt
     7381 aaatgccatc cataaaaaga tatccggagt gcgtaaagtc aatggatgca ggggaggcaa
     7441 attccatggc agggatatgg tcgatgtcat cagccctgcc gtttgtgata tattgtccga
     7501 tgacctcatt gatccgggtg cttaatggga tacggttact ggtcttacgg ttaagcagat
     7561 tgtagagtac atccgttgtc agttcattat catcctccgg tgtgatgaca tcattaccac
     7621 actgcttcag gtaatttttt gcggtgcgtg ctaccgtggt taatgcagag atagcttcgg
     7681 cttccatatc tttcctgccg gttccagcct ggggttcata ttcaaaggca ataaaaaaac
     7741 gccttgttat ggcgtctttg gaactgatgc gcttgatcag atttttataa tctttttgca
     7801 gggcgataca gcgttcatcg gtttctttac tgatgtcatc ctcaatggct gcgagatgtt
     7861 tgttaatatc cgctttcttc gtaagtacct tgaactgaag tttcaccgga ctgattttaa
     7921 ggaagctgac aaaggagtag ataatgttgc gctgttctct actgcttcgt agcaaaaaat
     7981 tgataggctc gacctcgatc agttttacat agcggtggtc tttcgtgtag ataatgccgt
     8041 tttctatctt ctcaatcggc agatagtcgg caacggggtt taagtagtgg tattctgtct
     8101 gtttatcggt tgcagcggat cttgcgttct ctcttttttt aggggaatgt ttttcttctt
     8161 tgccaaattc ttctgcaaac ttttcttttt tgggtttgga gcgtttcttt ggtctggaat
     8221 cggaaatatc cttttctttt gtgggttcac cagctccgac aattcttttc ttcttcaaaa
     8281 atataatgaa atgaaacaga aactcagtca ggctttcccc gccgatacca atgatggcaa
     8341 acagggctgc aggaagcgcg gtgatgcagg caatgataat gcgtgtcgtt aaggaaaacg
     8401 gcagatgaaa taccggaaat ccaatcataa ggaccagacc cagggcttcc acggcattac
     8461 ggagttttac ggatccacca agaaccgtgc cggtatctat aaagttcggg ggaatgatat
     8521 agacatcatt ctcgtcatta tttggttgca tgaaattact cctttcgggt tattgatagg
     8581 gccgcccaca cagaacgata ttgccggtac tgtaaatggg gcggtagacc acttttttct
     8641 tactgaggga ggcatcaatg attttgccat ctccggcata gatcccgaca tggctgatat
     8701 tcatatatct gttattcacc tcaaaggaat agaagaccag atctcctggt gatagcatat
     8761 cgtaggagat agccagattg ttttctacga tgtagcgtcc ctgggaagct gcggtatcgg
     8821 atccttcata ggataagttg atgccgcact gctcgtatac ataatgggtg aaagaactgc
     8881 agtcaaaata gccctcttca ttgcgcagaa cctggctgta aggtgtccca agcttagtaa
     8941 gcgcaatgtc cacagcatcg acaccggttt tgccgggagg gagatcttca ttgatgtcta
     9001 ccttgtagac ggtatcaaat aaaaacagat gaaggtttgc agcatagtca gccgccagtt
     9061 ctttctgctc atcagttaat gaaaaaacaa tgtctgcaaa ataatccatt ccggaatact
     9121 ctatgacgta ttcgtgatga taaacggtat attttttgcc ccgtccgtct gtttcttcac
     9181 gttccgttac attttccgta taggtaaaca gggtggatgc ccggcttttg atctgtgaca
     9241 ccagcctgct taccttaatg tttctataat cattctcgga agcacagtat tgtgatatga
     9301 gaagagtgct ttcgtagata atgcggtccg aaaaatcgtc agtgatgctg tattcatgat
     9361 ggagacctaa accgcttgcg catacttcaa tcgcatggat cgtctcgtca tgtttttccc
     9421 gtagtgctgc ttccacccgg gattcaatgg actcgatatt ctgcatgatc ttggaattgt
     9481 cattcaagac atctttagag gcagtatcca tgccctctgt accaaaaatc agagagggta
     9541 gcagtgacag gtagagcacc gggattaaca gcaatgcacc tacggcaagc acaatttttg
     9601 agattagatg ccggctttcc cacagtccgg cagcaagagc tccgtacggt cccgcagaag
     9661 ctccttttgc aatattggca atggctttac cggtttttat tgcgcctttt gccatagatg
     9721 ccgcacttgc aatgttggac agggtgttct tttcttcttt tgccattaca cattatcctt
     9781 ccgttttggc atatcgggga gctttcttat aatcgtctca tggtatgcaa tctcacccgt
     9841 gggtgtcata taaggagtct tctctacggt gggggcggca tactgcctgt accaaacgga
     9901 gttatcctgc atggttacgg tatcgtaatt ccccttcggt gtcatatatt gctcggaact
     9961 atacattcca aagctgtcac cgtttggatg cgcaatggag gtttccgtac ccatgatgcg
    10021 tccaccgcca atctcaacgc cggaaaagct tggtgcatcc tcttttccga catgacccat
    10081 gtaggaggag agagcttttg ctgcctgctc cccctgcata ctgccggtca tattcatact
    10141 gccctgggca atcgcagctg tgatattgtt tgcaaaggct ccgcccttgt taagggagga
    10201 ttcatagatt ttccggctga tcggattgga tgtgtgtccg gtcatggtat taacagtact
    10261 ctgggtaata tgcctgccga cggcacccac aagtcctccg gataaaaagg atgcctgatt
    10321 ttgtaccgaa ccgccctgga tggagccacc gcggaaaaag gaacctccat ggagcgattt
    10381 tacggaagtg attcccctgg cagcaatcat aagttcggcc atcatggatc caccggtacg
    10441 tcccacattg actccgagag atgccataaa ggagtcgatt ttctggctga cctttagaaa
    10501 agcaatcgcg cataggaacc agatgaatac tccggcagcc gattgctctt ttccaaccgc
    10561 agctacttcc tccggggtta ctgccatgac agatagtggt ataagataat agagggtgat
    10621 gccaaggatc agggtattct ttaatcgttt cattgagacc tcctacaaaa atagcggatt
    10681 cgacaggaac gaaccaacca tggatgtaaa tagttttaag caccaggcat tcataatgag
    10741 caaaaacacc tgccctgcaa gcatccgaca ccaggaagaa aagatgttgg aagtagactt
    10801 tgaagcaccg caggcaaatg caatcggggc ggtatatata ataaccccca gtaaaacata
    10861 ccgttctgca gcctcaaata acagcttgat atagttccat gcaagaacta aaagcaggat
    10921 cagtgcgatg atgccgatac ttccatttga gatgacagtt aaaatggtaa tcaggactgc
    10981 gttgaaatct gcgaagttta gtgctggcaa ctctgaagtc aggatccagt cgtagggagt
    11041 gcctccgatg gacagtacaa gatctgtaat ttcatcgcag taataaatga ggccgataaa
    11101 caacaccgat cggatgctta atttcaccgg atcctcggct tctaccccgg caatcagccc
    11161 aaagttctta aataactgcc agatccagtt aagaaggacc agcccaacgg ctgtggcaac
    11221 aaaaatactg tacatggttt cggctgccgg gaagaaacgc agaaaggtat tcatgtcaca
    11281 gcccaaagta ccaaggactg atgtggtaat gagatccagg gcattcatga cctgctctga
    11341 gacccattct acaatgccgt ctaatatacc catggctcag agcctccttt cctagcttgt
    11401 ccacttgcca cctgcaaaga acggagatac ataggccatg atgaaaccca gaccatttag
    11461 gatgcaccag gtaatcgcaa tccgtttgag ccatgccctg gattcgtcca cggttttccc
    11521 ggattttgaa aagttcataa gaagcagtgc ggttgaggct gtgacaatgg cggcaatggt
    11581 ggatatacca agaattttag tatagacatc tttcataatg tcttctgctt tggtccacat
    11641 atcagtagct agaaccggat ctgacagtaa gacaaagaag taagcggatg cccccaaccc
    11701 gataacaagc tgggggtaaa ggtttgtttg ctttcgtttc acgtaaggtt cctcctgttc
    11761 tgacacaaaa agagccggta tccaaagatg accggctccc gtgtgagata aaatttgagt
    11821 gtgattgatc agtatccatt ataacggtgg caaaattcca tgtaaatcgc ataacagtgc
    11881 cagtgttggc acaaagctgt gtcagtttta tgactgcggc ttatttgttg ggaaaaactg
    11941 gtttaacacc ttcaggcagt ccttatttgt gtatccccag agaacagcac ttaatgcctc
    12001 cacagcttcc ggacgtttcc ggtaataggt acgcctggag atattcaaaa tgtgtggccg
    12061 taagctttca acaatctctt caatgttctg gagctgctgg gggcagaggt aagtgtaata
    12121 caaaatccag tagtactgtt cgccgtgttt atgcttgctt ctcagagtat ctacggcatt
    12181 gtttagcaga tccaacatct tcttgctgcg agctatgctt ttggcataat tttcgatatc
    12241 ggttccatcc aaactggcac ctgcagcata cacagattcc aagaactctt ctatggtggt
    12301 gtcatattcc agctcaaagg tgttgcgaac ctgctgtact gcaagctcca ggttccagac
    12361 cgcatcgcgg tagtttttta ataacgccca ggtatcgtgg tataacggat tatcttccac
    12421 ggttgcatat ttctttttgg tgttcatcag gcaagcctcc cttccggagt ggtagtacca
    12481 ggttttgttc tggctgcatc caacgggtgg agtgctattt caaaacgata atgtgtcttg
    12541 ccatcgggac aactgatgga tagatagtaa tttaattcag aacccttgca gtctgataag
    12601 ctatataatt tatatactgt tttgggacat tcacaacagg aaagcccttg tgtagcaagg
    12661 attccggcta cttttttgat gactgcgcga atgtgcgatt ctgaaacgct agagatcttg
    12721 tctgaaacgc tgtttatgga tgcatcaata acaatctgct catcccggat agatgtatta
    12781 tccagaacgg aattgtcatc aggaatgtgg ataggtactt tgaatgtcat ggagacctcc
    12841 tctttatcaa tcattacgtc gctgattgcg aacatggtgg ataggtaatt gcacaagtag
    12901 atgacactac cgtgcctgcc gctaaaggat accagtgata agtagccgat tccctgcagt
    12961 ttattcaaaa gcctactgac cgaagccttg gatatccccc atctcgaaga gagatcagaa
    13021 tagcttatga gtggattttt agtggagtta cgaaaatata cgactggtcc gatgtccgat
    13081 ccctggatct gggtatcgtt gtaaatggca tggagccaca gatccagtac gatgtccatt
    13141 tctgaacagc gtccaagact gattagttcg tgtgcaatgg aaatcgggaa aaagaaaaat
    13201 cctatgtctt tcgcacaggg atagttatat tccagccagg tgttattctc tttccagtca
    13261 gaaatagtaa acttgataag atttccgcgg gctagcttac agtaggagat gtaatgttca
    13321 gtctggagat agtcgagtat tgccagtgcc tgatgctgga aacggacacg aaaccattcg
    13381 gagagatccg aaagtgtgca gatccattct ccgggactga ttaaatagga gattccctca
    13441 atgcgcctgt tggagcttct gaagttcgca aacgagcaaa ggaccatgta ataaaaaaga
    13501 taggaacttc cgtttgtccg gatgttccta tcttccataa gatctctaaa aaagcttctg
    13561 taaatacgac agcgtgggta atccacgatt tgtttaattt caatttggta atccatgatt
    13621 gacatcctcc tcaatatttg tcatggcagt gaactgaaac tgatatggca cagtcccaaa
    13681 caaaattcgg atagtatccc tccaaatgta atttgaaagg cattttttca actcagttta
    13741 gaactatgat atttcggggg attttgcaca aaattgttga tagaatggat atttaaaggg
    13801 atattgcatt ccggttattt ccattcatgt cgtaaagatg gtattgctgg tctaggactt
    13861 aaaatccctc gggggcaacc ccgtgccggt tcaagtccgg ccaccagca
//
LOCUS       DAWEJW010000077        13492 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1660_length_13492_cov_52.044951, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000077 DAWEJW010000000
VERSION     DAWEJW010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13492)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1660_length_13492_cov_52.044951"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3239 a         3635 c         2813 g         3805 t
ORIGIN      
        1 actaactttc catttaacgc aaaaacacgc tgcctaaatc ggcagcgtgt cccgttctta
       61 tttttacgcc agcatcatgc ggtcattggc gaattctccg ccgctgactt cctcgaactt
      121 tgccagcaga tccttcacct gaagattctt cttctcctcc ccagccacat cgtaaatcac
      181 tttgccttcg tgcatcatga tcaggcgatt gccgtgaacg atggcatcct tcatgttgtg
      241 agttaccatc atggcggtca gctgattctc tgcgatgatc ttgtcgctga tctccagtac
      301 cttggctgcg gtcttgggat ccagtgctgc ggtatgttca tccaacagaa ggagcttggg
      361 ctgcttcaat gctgccataa gcagtgtgat ggcctgacgc tgaccaccgg ataacagccc
      421 caccttgctg gtcatacggt cttccagtcc caatccaaga gtctgcagct tctcgcggaa
      481 taactctctg tcctctctgg aaacacccca gcgaagggtt ctcttctcac cgcgacgtaa
      541 cgcaatcgcc atgttctctt cgatatccat ggtagctgcg gttcctgtca tgggatcctg
      601 gaatactctt cccagatatt ttgctctctt gtgttctggc aggttcgtaa tatccgtacc
      661 atcaatgatg atcttgccgg aatcgatggg ccatacgccg gcgatagcat tcagcgtggt
      721 ggacttaccc gcgccgttac caccaatgat cgtcacgaaa tccccgggat tcagatttaa
      781 gtttactcca ttcaaggcca ccttctcgtt gatggtgcct ctgttaaata ctttatgtac
      841 gtcctgtatc tccaatgcgt aagccattac tttgacgctc ctttccgaag tgttctcttc
      901 tccttcaagt aaggaattgc caggaaaatg gcaactacga tagcggagaa caatttcata
      961 tcctctgaag gcatcttcag ccacagtacg aactgatata ccatatagta gatcacagcg
     1021 cccagaataa ccgatgccag ggtaaatgcg aacgcaagct ttctcttgct ggcaaactta
     1081 ccgaagagaa cctcaccgat gataacagct gccagaccga taacgatagc accgcgtccc
     1141 atattgacat ccgatgcccc ctggaactgg ccgtacagtg caccgcacag tcctaccaga
     1201 ccgttggaga tcatcagtgc cagcactttg atgaagttgg tattgatgcc ctgtgctctt
     1261 gccatattac cgttacagcc cgtagcacgg atggcatgtc cctgctctgt accgaaatac
     1321 cagtacatta cagcgatcag tgccagtaaa aatacgatca ttcccaggaa aaagagcagt
     1381 ctgctgacag agccatccgt cacatagcgc agagatacta ccagcggata attatctacg
     1441 ctgaccggct ggtttgcttt gcccatgata ttcaaattaa tggaatacag ggagatctgc
     1501 gtcagaatac ttgccagaat tccggggatt cccagtgcgg tgttcaacag tcccgttacc
     1561 atgcctgcca atgctccggc caggaaagca atgggcagcg tagctatagg agaaactccc
     1621 gcacgaatca gcataacgga taccgcagcg ccggttgcaa tggaaccatc cacggtcaga
     1681 tcagcgaaat ccagcaggcg gaaggtaata tacactccca gtgccatgac tccccagatc
     1741 attccctgtg ctatcgatcc cggcattgcc ctgaacaggg aaaggggatt cagtgacata
     1801 aagtaagaca tttctttttc ctcctaaaac tccagatagg gatatcgctg ttacgcgcta
     1861 tccctatcga agtatatttt gttagaaccg tacttaaata cctgtataac tcttactgaa
     1921 ttgctacgta atcatcggga acggtgatac ccagctcctc acagatcttg gcattgtact
     1981 tcttggtaac attgggagca taacgtacat ccatagtagt gatgtctgcg ccctcagcca
     2041 ggatctcgta agccatctca ccggtagcat atcccagatc gtaatagctg atggataagg
     2101 ttgctacgcc acagccgttg cagataccct cttcacctgc aataacagga atcttagcgg
     2161 gagctacgac attatataca gtctcggtta cggatgccag tgtgttgtcg gtagggatgt
     2221 acagaacatc gcactcgcct gcggcagccg tggtaacgga ctgtacttcg ttggtatcgg
     2281 cgatagcata ctctttatat gcaataccat ctttatccag agcagcctca aacagctgga
     2341 tctgatacag agagttagct tctgcggagc aatataacag accaacctgc tttacatccg
     2401 ggaacagttc ctttaacata gcttcctgct cttcaatagg agccagatca gaagtaccgg
     2461 aaatatttct gccggtagca ccggaccagt cggagatctc cagtgcggta gcataatccg
     2521 ttacggaagt acctaagatg gggatcgtgg tggtagctgc tgctgcactc tgtaaaggag
     2581 aggttgcatt ggcaaggatc agatccacac catcagatac gaacccatta cagatggtag
     2641 ctgcggtggt ctgctcaccc tgtgcattct gcagatcaaa ttttacatta tcaccaagct
     2701 tttcttttaa agcatcctgg aatccctggg ttgctgcatc cagagcttca tgctcaacca
     2761 gctggcagat accgatgtta tataccttac catctgtgga agcggcacta gtagctgctg
     2821 cttcactctg agctgtgccg gtagctgctg cggtggaagc agttccgtta tcggtgctgc
     2881 cacaacctgt caggacagat gctgccatta ctgcggtaag tactacagac aataatttct
     2941 ttttcataat aaatgtcctc ttttctgcgg atcttacgat ctgcttactg taatgcgcag
     3001 acgctttacc ccgtcaaagc gttgcactga taaatatcat aacccataaa aggacattcg
     3061 tcaatcaaaa ttcatcgatg ctttaaataa taatgatcta atttttagat attatctttt
     3121 ttcaccacat ctccgggtct tttgaagata tgtccggcag ggatcacccc tctgacacag
     3181 gaagtaggat acacgttgga attgcgtccg atgactgttc cgggattcag tacgctgttg
     3241 caacccactt ctacatggtc tcccaacatg gcccccatct tcttcagccc ggtttcaatc
     3301 ttctcttctc cgttctttac aaccaccagc gtcttgtcgc tcttcacgtt ggaagtaatg
     3361 gaaccggctc ccatatggga cttgaagccc aacacactgt ctcccacgta attataatga
     3421 ggcacctgta ctttattgaa cagaaccacg ttcttaagtt ccgtggaatt gccaactacc
     3481 gcgcctttac ccacaatggc attgccacgg acaaaggcac agtgacggac ctccgcttct
     3541 tcatcaatga tcagcggacc gttcaggcag gcggtaggag ctacctttgc gttctttgcc
     3601 acccagatat cctcgcctct tttttcataa atttcttccg gcagtctctt acccagttcc
     3661 agaatgaaat cgtggattct gggaagcacc tcccagggat attctgcctc ggtgaacaga
     3721 tctgccgcaa tggtctcctt cagatcatac agattctcaa tcgtaatatt ttccatgata
     3781 atttctcctc atctttattc cttatttttg gcattggatt gctttgtctt cttctgataa
     3841 aattcttctg tagcctggaa ctgtctccaa agcactccct gattgttcag gctgcgtaca
     3901 gcggtagttc gccttcccac aaaatccgtc agctttccca tgtactcttc cggtgtgatc
     3961 acaccatccg ccaccatggt aagccccttt tcccaactgg ccgtcagtgc cggatttaaa
     4021 agtggtctta tggaatccga taccacgtca tagatcatct ctcccatctg ggtgggagtc
     4081 agcacctggg tcttcttgtt cagtgccaga tattcgatat tcaccagttt cttcaggatc
     4141 tccgctctcg tggcgctggt gccgatgccg cttcccttga tctgggcccg cagttcttcg
     4201 tcctcgataa actggcccgc attttccatg gtcaggataa tgcttcccga agtgtaacgc
     4261 ttgggtggcg aagtctcgcc ctccttgatc tcataggtat ccgcagtcag agaatctcct
     4321 tttttcagat gcatcagcag attggtaagc tctgcatcac atttttcatc agcgctttca
     4381 ttctcattct ctttatcttc cttcggtgct gtattctcct tcttaaaaga gaagttcgcc
     4441 accttcagat acccttcctg taccagtacc ttaaagctgg caaaaaagtt ttcttccttt
     4501 accttcactg tcagagagaa cttctgatag actgccggag gatagaaaat actcaggaac
     4561 cggcgtacaa tgacctcgta cacttttgcc gccgtagggg ccagacctgc cagattgttc
     4621 agtccctgtc ccgttggaat gatggcataa tgatccgtga tctgcttatc attcacgtaa
     4681 cgtgtcttcg caatattctt ataggtcccc tgctctaaca cttctttggc aaaaggagtg
     4741 gccagagcat agctgcgtaa gccaccgata ttcttgtgga tttcctttgc caccgccgta
     4801 gacaggactc tggcatccgt tcggggataa gtggtcatct ttttctcata cagttcctgt
     4861 acgatccgca aagtctcatc cgggctgatc ttaaagtatt tggaacagtc attctgtaac
     4921 tccgccagat tgtacagaag cggtgcattc ttgttctctt ttttcttctc aatgccggtg
     4981 acagtcaccg ggcccaccgg ttctgcggta agctctgcga tcagacgctc ggcatcctct
     5041 tttttcttga agccattctc tttatacagt gccggagaat tataatattt cgagccttcc
     5101 actgctctcc actctgcggt aaagggcttc tcctgtaatt taaaattacc gatcacccgg
     5161 taaaagggag tcttggtgaa cgaacggatc tcccgctctc tgttcacgat cattccaagg
     5221 acacaggtca tgacacgacc cacagaaatc actgcccggt ctcttttgag ataccctttt
     5281 accgtgttgc cgtattttaa cgtcagggct ctggaaaaat tgatacccat gagataatct
     5341 tccttggccc gcagataggc agcatttccc agattgtcgt aggctgagag atcttttgcc
     5401 tcccggattc cacgtaagat ttcttcctcc gtctgggagt cgatccatac acgtttccgt
     5461 tccttgtcct gtacgcccga catctgctcc accagacgat agatgtattc tccctctctt
     5521 ccggagtcgg tgcagacata aattgtcgta acatccggac ggttcagcag ggaacttacg
     5581 atcgtatact gttttttcgc actttcaatg acttcgtatt tgaattccgt cggtagaaaa
     5641 ggcagggtgt caaagctcca tctacggaac ttttcatcat acacctccgg ataactcatg
     5701 gtcaccagat gtcccacaca ccaggtaacg atagcctctt ccgattccag atagccgtct
     5761 ttggaacgca tattgtattt taaagcattg gcaaattctc ttgccacgct cggtttctct
     5821 gcaataaata aatttttacc cacgtaatac catctttcct gtcttacgag ttctcatcca
     5881 cggaatgcac cagtatctgt accgttcctt ccgttaatgt cttctcatat ccattctcgc
     5941 taaatatcag aatgctgtct gcagtcacat gttccctctt catggatgcc tgcagcacat
     6001 cataatcttt cgaagtataa cataccgtgc cggaataatc caccacgatc tttctgccgg
     6061 tgtcctccct gcacagaaaa tagattttcc cctgtctgct gttccagagt tcaaccgtcc
     6121 ggaaacgtcc ctgcagcact tcctgacaga tccggcgata ggtcaacagt tgaaaatcat
     6181 tatattcttc ccggatgtat ttttcataaa atgtactgcc ttctgtccgc tcagacgtct
     6241 gatgcggaaa caggaagcaa aaataaaacc ggacaaacgc attacagatc tcatatgtac
     6301 cgggcagcac cttctccacc agctccagtt ccatcaaatt cttcagatat acactgatct
     6361 tggcccggga aaatccggta tgtgcataca tatcgttcag ttttccatgt cttccgtctg
     6421 ccagcgttgc cagaatggtg tcataaactg ccgtctcccg caattcctcc gacagccatt
     6481 ttatcataag ctccggcaga aagctgttcc tctgtaaaag cagccggatc agattttctt
     6541 ctgccgaatt ctgcggataa agcagccgcc acagtcccgg cagccctccc aatgtcgcat
     6601 aaagcgaaat actttcctgt actgtatagt cggaaaatat cttacgcatg gcggaaaaag
     6661 gcagttcctt caccttcaga aatccgctga cggaaaatgc cagatttccc actttggaaa
     6721 tcatgctgtt ctccacccag ccggaggcat aggtggtcag gatcactaac agtgcttctt
     6781 ttttctcctt caggaaccgg atcagttcct gcaaaaacaa agtatcccct tttaaaagat
     6841 actgaaaatt ctctatgata agtactttct tatctgctgt cgtttcgcct ttctgtattg
     6901 tcatgcattc cagcaattcc cgccaggaag gatactctgc aatctccttt ccccggcttc
     6961 gcagttcccg tccccacaaa tattgttgtt ctctggaaga acagtttccc gcactataac
     7021 gcaacgtctg ttttttctcc gtaaaagagg ttaagagagc cgtcttgcca acgcctctct
     7081 gtccatacac caccaggatc tgactccctg ttctcttata atgattttcc aaaaaagctg
     7141 tttcctgttc ccttccagtc agcatggcag cctctttctt tctgacccag cagatcagta
     7201 catcttctgc aacagttgtt cgatctcttc ctgtgacaac ggctttccaa acagatatcc
     7261 ctgaatgata tcacacccga tagcccgcag atatttatac tgctgttctt cctccacgcc
     7321 ttccgcaatg gactcaaatc ccagggattt caccatactt acaatagact ccgtaataat
     7381 ccgggtcgca gaatccgtca gcagcgtgtc ggtaaatgat ttatcaatct tcagcgtatt
     7441 gataggcagc ttctttaagt aagacaggga agaaaatcct gttccgaagt catccagtga
     7501 aatgcgaatc ccgtactcct tcaacaactg cattttctcg gtaactgcac tgaaatcgtc
     7561 gatcaaaatg ctttctgtga tctccagttc gatttcatcc ggatccacat cgtatttatg
     7621 aatgatattc agcagggttt ccacaaaatc ttccttctgg tactgcaacg cggaaatatt
     7681 cactgacagc acaaaaggaa ctccgtaacg gttcctccat tcggaaaaag tacggatact
     7741 ctgttccagt acccagttgc cgataggaat gatcgttccg ttcttctccg caatgggaat
     7801 gaattttgcc ggactgatca ttctgccgtt gccatccttc cagcggatca gtgcctccac
     7861 gccccgcagt ttacggttgc ccgcataata ctggggctga taatacagca gaaaattatt
     7921 ctcaaaaaca gcctctttta atttgctgtc cagttctaca tttttcagga aatcgttcag
     7981 gatcggcgta tcgaaatact ggatccggtt cttgcccatg gcttttccct tgaacattac
     8041 gatttccgca cagttgatca gttccagtgc ggaagtcgca gcctccggat attccgccac
     8101 accgacactt acggtgatgt tcagaacctg cccgcccacc agataaaacg gctccctggt
     8161 gcgcttaacg atcttcttat gaatatgttc cacacttttg ttcccacaag gatcataaat
     8221 ggccatgcag tagacatcac tggtcaggtg gcagacgatc acatcatccc cgttaaattc
     8281 cttcagaaaa gagccaaact gctgtaccag ttcgtccccc gccacgattc ccagtccgtc
     8341 atttaccttg cggaaatcat caatatcaat aacaagcacg ctgaccagcc ggttatcctc
     8401 tttggcccga cgaagaaatt cggtcagaag acgcacaaaa taattacggt tgtacagtcc
     8461 ggtcagactg tcgtaatacg ccatgtagag aagctcttcg ttctgtgttt tctgtttggt
     8521 gatattctgt accacaataa tctgatccgt cggcctgcca tcttcgtaga aaatcctgct
     8581 ggaaaactgc aaccaggtct tctttcccct ctgcatacat tccacagagt ccgtctcccg
     8641 accgctcttt tccaaaaaca gcatttcccg aagtgccagc acatacggtt cgtccaccat
     8701 ctcctgaagt tttacaaaat ctcttctgtc cagaatctgg aagtcaaaga aatcatccca
     8761 ttttcccagc gtagtgaccg tattcttttc aaaagaatag tagatataag cataatccat
     8821 ggtatcgcac aacagacgat acattctctc cttagcgctc agtttctgat tcattgcgcg
     8881 cataagatcc agctgatagc gtaactcgtt cataacccca ctcacccttt attttttggt
     8941 gatgtaaccc tgtgctttca aaagttctgc gcaaagcaat gctcccccag ctgctccacg
     9001 taaggtattg tgtgacaaac ctacgaactt gtaatcatat acggtatctt ctctcagacg
     9061 acctacactg atacccatac cgttctcgaa atcaacatcc agagccacct gaggtctgtt
     9121 gtcctcctcc agatactgga taaactgctt cggtgcgctg ggaagtccca gtctctgagg
     9181 ctcaccgctg aaggtgacca gtttctcgat cagctgctcc ttggtgggct tcttacggaa
     9241 tttaacaaat acggctgcgg tatgtccgtt caataccgga acacggatgc actgacaagt
     9301 gatcacggga gaagttgcgg gaacgatctt atcgccttcc actttacccc agatacgtaa
     9361 gggttctttc tcactcttct cttcctcacc accgatgtaa ggaatgatat tgcctaccat
     9421 ctcgggccag tccttaaaag tcttgcctgc gccggagatt gcctgataag tggttgctac
     9481 cacttcgtaa ggctcgaatt ccttccatgc ggtcagaacc ggtgcatagc tctggatgga
     9541 gcagttgggc tttacagcga taaatcctct ggtggttccc agtctcttct tctgtgcctc
     9601 gatgatctgg aaatgttcgg gattgatctc gggtactacc atgggaacat cgggggtcca
     9661 tctgtgggca ctgttgttgg ataccacagg ggtttcggtc tttgcgtatg cttcctcgat
     9721 ggccttgatc tcatccttgg tcatatctac cgcactgaat acgaaatcaa cctgtgcggc
     9781 tactttttct acttcattga cattcatgac taccagcttt ttcacagctt cgggcatggg
     9841 agtggtcatc ttccaacggt cacccacggc ctcctcataa gtcttgcctg cggaacgagg
     9901 gctggcagca atggtcgtta cctcgaacca gggatggttc tccaacagag agataaatct
     9961 ctgacctacc ataccggttc cgcccaggat acctacacgt aatttttcac tcatttttca
    10021 tttcctcctc atctttgtgc atttttattt tatctgcaaa agacctgttc agtttccact
    10081 tactgaatgc ggtcttccaa agcctgttga tatacttcta tggcatttgt tttcaggtat
    10141 tctttatttt tctcaatgac ctgcttcatc atttccgcac cgggagcacc gatggtcagc
    10201 cgcttttcca cacaggtctt taaggaaatg gcatcgtaaa tgtcctcatc aaacttatca
    10261 ctgatggaac gtaattcttc caggctcagg gcatcaatgg aagtatcctt ttcaatacaa
    10321 tacagtacca ggcgtccgat aatgccgtga gcatcccgga aaggtacgcc cttacctacc
    10381 agataatctg cggcatccgt agcattggta aatccgttca tggcactctt tgccatgcgg
    10441 tccttacgga acttcatggt ctttatcatg ccggtaaata aggcaatgca gtccgctgcg
    10501 gtatccatgg catcaaaagc catctccttg tcttcctgca tgtctttatt atacgcaagg
    10561 ggcagtccct tcattgtggt aagtaacgcc atcagggcac cgtagactct tccggtcttg
    10621 cctcttacca gttcggcgat atccgggttc ttcttctgag gcataatgct gcttccagtg
    10681 gaatatgcat cgtccagttc cacgaactgg tactcattgg aattccagat aatgatttct
    10741 tcgctgaaac ggcttaaatg catcatgatc gtggaaagtg cggaaaggaa ctcgatcaga
    10801 taatctctgt ccgccacaga atcgatactg tttaatgtcg gtccttcaaa acgaagcagc
    10861 tttgcggtat actcacgatc taacggatac gtggtaccgg ccagtgctcc tgctcccaga
    10921 ggacaataat tcatacgttt gtaaatatcg gccagacgct gtctgtccct cttgaacatt
    10981 tcaaaatagg caccataatg atgagccaga gtaatgggct gtgccttctg taaatgggtg
    11041 aatcccggca tataggtctc taagttgttc tccatggtgt ccagaatggc ttccagaagc
    11101 ttttccagaa gtccgtctgt ctctgccact ctcgctctgg tatagagacg catgtccaac
    11161 gccacctggt cgttacggct gcgtccggtg tgaagctttt tgcctgcatc accgatccgg
    11221 tcgatcaggg ttgcctctac aaaactgtgg atatcctcct gggtttcgtc gattgtaagc
    11281 tttccggcat ccacatcctc taaaataccg gtcagtcctt cgataatgct ctttctctcc
    11341 tcctcggtga ggattccctg ctttgccagc atggtggcat gtacgatact tccggtgaca
    11401 tcttcatgaa acaatctctt atcgaatgtt atggacgcat tgaaatcaaa taccagctga
    11461 tcggtctgtt tcgtaaagcg tcctccccat aattgtgcca tggtcattct cccatctttt
    11521 tattctgtca gcagcctatc agacaatttt tctcatagac attgattgca attaatcata
    11581 tcacaaacga cactttaatg caatacagtg aatttcatca ctttcgggta gcaaaaacac
    11641 ttttttcttt taaattcata ctataacaat aaatattcaa gttaaggaag tatgcctttt
    11701 tatgggagct ctcatcgaat taaaaaacat aggttactcc tatcataatc tggcgggcga
    11761 gaccgatgcc ataaaagatg tctcctttca ggtggaggaa ggggaatttg tcgctttagt
    11821 aggtcccagc ggctgcggga agtccacgct gctgtctatc attgcgggac tgcttgctcc
    11881 ggagaccggc accatcgtag tcaacaatcc cgacggatcc cttcactatc cccgtgtcgg
    11941 ctatatgctg cagcacgatc atctcttcga atggcgtacc atctaccaga atgtcaccct
    12001 gggactggag atccatcatg ctcttaccaa agagcgtacc gactatgtga aacagcttct
    12061 gtccgactat ggtctggaaa gctttcagga taaacgtccc agcgaattgt ccggcggcat
    12121 gaagcagcgt gctgccctga tccgcaccct tgccctggat ccccagctgc tccttctgga
    12181 tgaacccttc agcgccctgg attaccagac caggctcatg gtatccgcgg atatctgccg
    12241 gctgatccgg caggctggta agactatgat catcgtcact cacgatctgt cggaggccat
    12301 cagtctggcc gacaaggtct atgtactgtc tgcgaggccg gcgacgatca aggccgttct
    12361 ccccatccgg ctcacgcttc cggatgcatc accgctggct gccagaaatg cgccagaatt
    12421 tccatattat ttcaaacaac tctgggagga attaacggat gagaccacaa actaaacaac
    12481 ctctgaccgt tcaggaacaa tatcttcgaa aacacaaaag ccatcacaga caggtctcct
    12541 tcctgcgctt ttttcttctg ggaatctttt tactcctgtg ggaagtaagt gcagatcttg
    12601 actggatcga cagtttcttt ttctccagtc cccacagagt cattctgtgc cttggagaat
    12661 tatggacaga gcggtccctt ccgatgcata tcggtatcac actgtcagaa acgatcctga
    12721 gtttcctgct ggtatttttc gtcagtcttc tgttatccac tcttctgtgg ttttccaggc
    12781 agctgtcgga gatcttagag ccttatctgg tactgctcaa cagccttccg aaatccgctc
    12841 tggctcctct gtttattgtc tggctgggca ccgggatcaa caccatcatc attgctggaa
    12901 tctctgtggc agtattcgga tccatcatca gcttttacac cgcttttcat caggtagatc
    12961 ccgaaaagga gattctgatc cgcacactgg gtggaaaccg cagggatatc tttttcaaag
    13021 tggtactgcc gggatccatt cccaccctgc tcagtaccac caaagtcaat atcgggctgt
    13081 ccctggtagg tgttatcatc ggggaatttc tcgccgccag acggggactg ggctacctga
    13141 tcatctacgg ttcccaggtc ttccagctgg atatggtaat cagttccatc ttactgctat
    13201 gtgtgattgc catgctgctc tacctgctga cacagtctct ggaacattac agcaaaaagc
    13261 accgcaactg acctttcgga gatccgcagg cgttttcgtc ttacggtctc ctacataatt
    13321 tacggctttg tgaaacgttc tgcgaaaata ataaatttta cagttgcaaa atttcccaag
    13381 aacatatata ataacctagt ccgctgaaat agctcagttg gtagagcact tcactcgtaa
    13441 tgaaggggtc gtgggttcga gtcccatttt cagcttatat gtattaaaaa ag
//
LOCUS       DAWEJW010000078        13453 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1669_length_13453_cov_62.846917, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000078 DAWEJW010000000
VERSION     DAWEJW010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13453)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13453)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13453
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1669_length_13453_cov_62.846917"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3324 a         3674 c         2732 g         3723 t
ORIGIN      
        1 acgatcttgc ggaacgcctg ccatttcacc gggaagctca cataatccac cacataacct
       61 ctgcgaaggc ggtcataggt attgctgaag gcaccaccaa gcaacagcga tagtcccagt
      121 cgcagcaaat tgttgcctcg acgtcccaga ctcacaataa agacaacggt caatatcagc
      181 gtaaatgcca gtgaaacagc agccaccaga cgccttctct tctgtccggc attgagcatc
      241 atgcctttgt tatgatgctt gcggatcaga aattttccct tgcagattac tcgttcgtcc
      301 cgctcgtcca catgcgactc tatataattt ttcaggaaaa aatctcccca gaaaattcct
      361 gccagtatca caagacatac cactgtcata tgttccgctc cttattcacg ccgtccgtat
      421 ttccccttgc aatctgctta ttcgcactct gctttccgat tctttatctt caaaaacgcc
      481 agaagtgccc catacaatac tccggccaca ggccatacga cccaggtaat gtcccaacgc
      541 atcgtcggca ggctgatagc cagataaacc gctgtccaga cgcaccagta aattgctgtc
      601 acatgctcat acttccggtt gaccgcctta ttctccacag tgtaatcccc ctcctgaagc
      661 aatttctgaa aaccgcccca gataataccg gaccacacga acagccatac agcgatcgcc
      721 actacaatga gtaaagcatc cgtagcaagg attaccgcat aaccattatt tccacaaaaa
      781 atggatacta ccagcaaagg taccactccc agtatacaca atacaacacc cagggttatg
      841 cagatccggt aggttcccgc aaaggtttct tttgctttct ccacgattcc ggacactcca
      901 tattgcagtg tcagcttttc tttttccaga tattcatatt tattgtatgg gattccggtg
      961 agaagaaata ccgctaccgc aactcccacc attagtaaca gtacaatgag accgattcct
     1021 tccgcgacat tttctgtgac cgctcctctt ttggcacctg ccattcccaa caaccacaac
     1081 aatacaacag gagagagcac acataaggat actgccggag caatccgggc agcatatttt
     1141 ttcgtagctt ccagaaacgt attggcttcc tccaccgtga tcacttttcc ctctgtgatc
     1201 tccggtgtca ttccatcggc gaattccgtc tcttccattt catcttttaa taggtaatcc
     1261 gtagtaactc cgaacaactg gctcatactg acaatgcgtt ccagatccgg gattgaggct
     1321 ccgctctccc atttggatac ggactgtctg gaaatgtcca gacgttctgc cagttcctcc
     1381 tgagaccacc catatttctt acgcaacatt attatttttt ctgacaggat cattttcatt
     1441 ctctccttct tttattaacg ttttcttgtg cacacataga tcgttgacat gggaaaatca
     1501 taacaaaaga gccggttgac aactaccaag tgcccttgaa attttgtcaa ccggcagttg
     1561 cgtttccgga tttttctgaa attttcagca atattactgc acttcacgga tcagacagta
     1621 ttgctttagc gggaaattct tctccatcac accatatact ttgaatccga acttttcata
     1681 gaattccacg tttttcggat tatgagtctc cagggagatc atcagcttgt gtttctgggc
     1741 atagtcgatg gcttcctcca gaagctccgc tgcaattccg tgatgctgca tggatggatc
     1801 caccgccaga taaatgatat gcagacgctg attctgatgc agttgatccg tccagctgga
     1861 attcagataa tcctcaccct tcataaaatt ccgaaatgtc tttaacgaag gatcctcctt
     1921 gatcagatag gaatccgtct gtaaagcagc catggcctct gtaaaataga actgaaatac
     1981 attgtacggc tccgcttcat ccgataccac caatacactg ttcagatccg cgctgtctgc
     2041 gaagatctca caggtcgcat aaaattcatc catgtcacat ttaaacagct ccggcatcag
     2101 atttttacgg acttccggat ccggaatcag cgtctcgtac aacggatcgt gtgcaaagca
     2161 ctgattcagc attttctcca ctttcggaag gtcttccttc tgcacacgat ataacttatc
     2221 actttccata attgtattta ctgttatctc cctattatga tgatgtcagt ccccagcttt
     2281 gttgtctgtg gatcgttccg tgctctgctc tcgccatcat tacaattcga atatctctgt
     2341 cacagatctc tccatttgtt gcacgatatt catcaggctg accacaagct tgctgcatgc
     2401 atgctccagg ctggtaattt cttcccggta gtccatttcc ttcataccgg atcctgtcaa
     2461 catctccagt tgtttttctt tttggagaag ctgcttgacc gttccatata gattttcatg
     2521 ctccggcagc cttccgtaac cccatttcag gcgatccttc ggctgatgga atacataatc
     2581 cgtaaaatgc ctatatactt cttcatcaaa attaaaggca taggcattgt tgtctacctg
     2641 ataaagctta cgaagtgcgg aggaaagtct ctgttccttc agatttctca ccgctccgcc
     2701 cagaagtctc agatttgcac tgcatatctc atgaggatac aacacatttc cgtaccagtc
     2761 aatccgcaca aaagcgtcct gctcctgttg gaaccttgcg agcagcttct gttccagcgg
     2821 tctgatctgc cggaaaagtt tctccgcctc gtcgtatttt ccatctctaa gcaactgttt
     2881 gtagttacgg ttgtattcct ctaccgcttc atattcctcc cggcatcttc tgagtgcccg
     2941 gtcacttaaa gtctccagtt cctcaatttt ttcaagcaaa acccgttttt tctctcccag
     3001 ctgccccgcc acatcggcgc ggtagcagat ttcccgacac tgttccaagc ccttgcgaat
     3061 ccgctgcacc accggtgaaa actgcaacgg taccactgcc gtttcatcga gtgccaggat
     3121 caacagggca aacagctcat gatgcagacg atatacctgt tcatcataaa actcatcgtt
     3181 atcaaactga gaatggtagt gggtctccat aaagcttccg ccggtgaaat catttaccat
     3241 ggacggtaca cctgcgatcg ccatggaaaa atcatccgac caggtctcga tcggagatgt
     3301 cactgctgtc tcttccggat aggctatcgt cagattcggc agatccgcca gatattcctc
     3361 cagaaagctg acgtattcat agcagctgcg gatcctcgct ctggtaccat gcgccaacgc
     3421 agggagttca aagttcaggt ctgcgatcac cttgcccacc cattcgggat gtgcggtgaa
     3481 tatctgctca taagctcccg tagaccagtc gaaattggaa tccaccacgc cccattcctc
     3541 ggatgccatg gcacagatca caatggtatt gttcggttga aaaccactgt ccagcaacgc
     3601 ctttgcaata ccaaacatca gggcgaccgc tgtattgtca tcctggaatc cgtcaaaata
     3661 ggagtcatag tgggcagaca aaagcaccat gcggtcagga tgttttccgg gaatccttcc
     3721 cacaatatta taagtcgtgc agtttctggt caccctggat tccgcatcaa aggtgaccgt
     3781 tatttcctcc tgatcacgca gcagctccaa caggccggcc gcatcttttc tggaaatgga
     3841 aaaagcggga gcatcctccg gtccggcgat atcctgtgca ttcagagctt catcgtcgat
     3901 ttcgccgtag ccgccgctct gcactgcgat caaggctgct gctcccttca gatgtgcctg
     3961 gtataccgga taattgatcc accactcatc cctctggtta atctccacca gtaccagttt
     4021 atccctgaca tcttttccct gatagtccgt ctccgttcct tttcccagat acatcatgga
     4081 aaattccttc gggccatcgg tgacaaaggt tgtctgatag gctcccagct ctgctgacac
     4141 ggtctccccg gctgcattcg tatagcttaa cgccgcctta tgaaattccc agccgtctac
     4201 cgtcacggca tcctttgtaa cctcagacag tccgattttt tccatttctg tcttcagcat
     4261 ctctccggtc tcaaattctg ccttagaacc cgccgggcga taccccagtg tcggatgtga
     4321 ccggtattgt tccattcgct ttgccagttc atacgaatac ggtatatcca gattctgtaa
     4381 cacctttttc tgtaaagtca ccccgttgtc atgcatattc tgtcactcct gtttctatcc
     4441 tgtccgttct gtcacaggaa ccgttactta tttttttacc atagaattca tattttcagt
     4501 aattttctgc agaatctctc tgtttctctc ttccgcttcc agtgtctcac tggcatgggc
     4561 agacacttct tccgtagtcg ctgagattgt ctgaatggaa tccacgatct cctggttggc
     4621 tgttttcaga gcttctactg tctgaagcat ctcacccaga ttatccacaa tcgtatagct
     4681 gctgtcttca attgccgtga aactgtccgc cgcacgcatc gttccttccc tttggctgcc
     4741 gataccgtct atcatctgac ggatgaccgt caccacttct ccaatcgctc cggatacatt
     4801 ttggatcaac tccgcgatgt taccggtagc atcctttgtc tgggtggcca ttcccgaaat
     4861 ctccgtcgcc actacggaga aaccgcggcc ggcttcaccg gctctggctg cttctatgct
     4921 ggcattcagc gccagaagac tggtctgatt agctattttg ctgatgattt ccacgatgga
     4981 atgcatctcc tgcatataat gatccagcgt ttccagcttc tgtgccgcca gttcactgtt
     5041 tcttgcagaa tcctctgcct gctccttcag acctgtcatc tcttcttttc cggcatccag
     5101 catttctatc gtatgttcca tattgcttcc gatcgtctct gccacttcac ccacagtatt
     5161 tactttttcc tgaatagcct ccgtctggga cgtctgctgc tgaatcgccg ctgcagactc
     5221 cccggaacct gcagacacct cctccatggc tgttttggta ctgtcaaaag cagtttccag
     5281 tttattcaat tccgctgtga tatcagctac tgaagtctcc atccggctgc tcatttccat
     5341 gacctcacgt aaggttgctt ccgcgcggtc ctgcgcagct gtaatgctct ctatattttc
     5401 atcggtgttc ttctggttgg atatggtagc atagatggat gtaatgcaca gcagaatcat
     5461 taccgaaatc tgtataaatc ctgcgtcctg ccccagatag ccaaatcccc cttgtgtggc
     5521 tcccaggagc accaccagga tattctctac caccgtcccc acattaataa gtgcaaatgc
     5581 cttgacatca tgataaggca tgactgcaaa catcatggga attacaaatg catacaccat
     5641 gttgcactgt gccgtaaaaa gcagcaccgt ataaaacaac gcaaatccga tcagtgacag
     5701 atgttttata gctttggttg catgatccct cttccaacaa ataaattctg caagaaccgg
     5761 tatgatccca agaagcatca gcactaccaa taccggaacc gacactgtgt gttcaacaaa
     5821 tccgatcacg ctaatgaaaa gcatacatgc attgatcaca ccatgtgcta tcatggccac
     5881 ccggttactt cgttccaact ctttcatttg cattctcctt ctctcccgta tgatattatg
     5941 ccaggttcaa aaagtctaag cccatatgag cttgcttata agtgggtgaa agaccttaaa
     6001 ttcataacgt aattcctcac atcccttata ttgtaattca tgatggtatc cccgtcaata
     6061 aagcacacga aaatgcagtc cggttttcta tgaaaaacgg actgcatttt ctgtcattta
     6121 tatgttatct ccctgccgga cagcttccag caccctcttt ataccttgaa aacatccagt
     6181 accttttcca gcttatccgc taatgccaac aggttctcag aagaattcat cagttctgtc
     6241 atggtatcac tcatgccatg agccgcctgc atggtactgt ctgcggatgc tgcattctgt
     6301 tcggagattg ccgacaatcc ttccactaca tcacggatcg cacttccgga ctgtcctaag
     6361 acatccatct tctcccggat accgtcaata tgctgcaggg aattctccac accatccgct
     6421 actgccgcag tcttcacacc ggtctcctct aacttgctct gctggtgatt catgttggct
     6481 tccacttcac ccatgatctc caccattctg ccggaagact ccatgatatt ttggatgatc
     6541 tgctggatct cggttgcgga gcgcttagac tggtcagcca gcttactgat ctgctctgct
     6601 accactgcga agcctcttcc cgcttctccg gctctggctg cttcaatgct ggcgttcagt
     6661 gacagcagat ctgtctcgtc cgcaatatcc tgaatcatgg atactgcctt ggtgatattc
     6721 tgaattgctc cgttcatatc cgtaatctgt tccgctaccc gcaggacgga ttccttggtg
     6781 ctctcgttgg agccgttcag ttccttcatg atctctccgg aagctttctc cgcttctgcc
     6841 atcctaccgg cataatccgt cagagatttt acttcctcag atatatattc gatctgctcg
     6901 ccgatacgca ctacattttc attagcatcc gttgtctcat tggcctgatt ccttgcgccg
     6961 tctgatatct cgccgacagc atggtataca tcattcacag attcctgggt atcctgtgcc
     7021 atcttgatca gtttgttgga agatgctgtc agatcatccg cggaatcctt gatctctgtt
     7081 acgatcgaac ggagagtttt ttgcagtttt acagaaatac gatatacatc cccaagttca
     7141 tcctttttac ggcactgctt ttcatccggc tctgcatcca gttctccgtt cacgatcctg
     7201 cctaggaaat gcttcgtctt cttcatggtc ttcaccatgc tacgggataa tacgcttacc
     7261 agcgttgccg ccacaagaac acatgccaca gcgatcagaa taatatcctt cacctgcatc
     7321 gagattgtat ccttgacatt ctgcatcggc gtcgccactt cgatggcacc gatcacagag
     7381 ccatcagaat tgatcaatgg ctgataatat acataataat ctacttcgga aatgttgcag
     7441 tccgtcagga acaaagcctc cccagtctga atctgtgcat agacatcgtc ttccaacgcc
     7501 gtaccgttaa tacgtcctcc ttccggcttg gtcaatgtcg tgatcagacg catatttcca
     7561 tacaaaaaag acacctggaa gcctgtcttc tcctgcaatc catcgatcaa ctgtgtctcc
     7621 ccggaaatgg atgtgccgcc cttgcgcacc ttgcctccct tatctctggt gtaatcgcct
     7681 tcatacagat tcgtgtaggt ttcatccaca ctgacaccaa cgatctgcag agactgctca
     7741 atctgtttct caattccggt ctctaatgct ttggccccca ccgtagccac cagtgcacat
     7801 acaatgatca tcggcagaag gctcaacgcc agtatctttt tggaaattgt gaaaaatggc
     7861 tttttgttcc cttttacttt cgttttctta ttctctgtct tcttcatgct taccacatct
     7921 gcctttctgt aaaagacttt atcacattcg atccatctcg aaaacgttta agtattttct
     7981 cagtgaattc attgtaacat tttccatata gtaatacaat tcgacaaaat tacaagtatt
     8041 ttattgttaa atttggtcat ttttccttaa cgttaagtga aaatatctct ttttgtttac
     8101 gaaacagtta agttttctgc ttccagaccg gcatacgcag tatctttatc aatagcggaa
     8161 tgatcatgat aaaccacaca ataggctccg ccgcgatgac acccgggtat ccgaacatgg
     8221 gaaccagcgt cgccgcgatc acaattttcc ctatcatttc caacgagctg gagatcagcg
     8281 gtacgatccg ctctcccagt ccctgcatgg agtttctcac aatacagatt accgctgtca
     8341 cataatagaa cagtgtatct accttcagat ataatgtggc attgtagatc acttccggag
     8401 ttttgctgcc ggtgaccagc cataccagcc agtcaccgat ggtataagca gtcacaatat
     8461 taagcgtaca ccagatgcag gtataaccgg ttgccaacag gattcctctg cggatccgtt
     8521 cgattttgcc cgctcccaga ttttgtccgc aataggtcgc cattgtctga ccgaagacgc
     8581 tgaacatgat catgaagatc tcggagatct tgcgggctgc cgtatgggca atgataatat
     8641 tctgtcccag cttattgatg gaggtctgca atgtcaatgt accgatattg accagcgagc
     8701 tcatgaatcc catggaaaga ccggagctta acatattgcg gagcattccg gcatcctgca
     8761 gcttcagatc atccacacgt atccgcagaa tttcatactt ttcccacaga tagaccgcac
     8821 acaccaccac agcgatcagc tgcgacagca cagtagcaac tgcggcaccc agcactccca
     8881 tatgcagcac cacgatcaga agcagatctc ctccgatatt cagcaccacc gacaccgcca
     8941 gcacgatcag cggggtcacg gtatctccga tcgctcttag ggtcgccgct aatacatcat
     9001 atagtaatgt aacaacaagt cccgctatga tcacggatac atactgtgtt gctgtgtgca
     9061 gcagattttc cggcacattc agaaatctga ggatcggctg caaaaataca agccctccta
     9121 tcgtcagtac cacagctgcc aataggccaa gcaccagaga attgcccaag gatctgcgca
     9181 atctgtccat atccttcgct ccgaagcact gtgccgaaat aatagcaaag ccattgcaca
     9241 ttcccatcag gaattgtacg atcagattgc tcagcgtcgt cgtggccccc actgccgcca
     9301 gggcattatc tcccagaaaa gtacctacga tgcgggtatc tgccaggctg taagtaagtt
     9361 gcaataaatt ggccaaaaaa ataggaaggg caaacgtcag tatcaaaact gacggtctgc
     9421 cctttgtaag atctttcata tagttcctca cttctatact tccggaaaaa atatggattt
     9481 tccccgtgtg atagggctac agaacttttt taaaactttt tcttggcatt gtcaaggtat
     9541 ttcttggtct cagccaccac aacaccattt aatgccagca gtgcgatcag gttcgggatc
     9601 gccatcagac cgttgaagat atctgcgatg gtccatactg ccttaacagt catgtagggt
     9661 ccgatgaata cgcaggcaat gtagatccaa cggtatactt tgatcgcctt cggcttctga
     9721 ccgatcaggt attccaggca tctctcactg tagtaatccc aaccgaggat cgtggtaaat
     9781 gcaaagaata ccaggcatac catcaggata aaggatgcaa tagagctgtt aaaaggcagc
     9841 ccctgctgga aagctctggt ggtaacatcc acgccgtcca aacctacgtt ccaggaatcg
     9901 gtaattacga tcgtaagacc ggtcatggta caaatgatca gagtatcaat gatggtaccc
     9961 atcatggaga tcagaccctg tttggcagga gagtcgttct gcgctgcagc cgctgcaatc
    10021 ggtgcactac cgataccggc ttcgttggag aaaatacctc tcgcaatacc cttctgcatt
    10081 gccatgataa ccgctcccaa agcaccgccg gctacggcac gcataccaaa cgcactctgt
    10141 agaatggtta cgatagctgc cggaatggca gttacattaa agatcaatac taacagacag
    10201 gctactacat aagtcactgc cataaaagga acgatgacct gtgccacggt ggagatacgc
    10261 tttaatccac cgataacaac cagtgccacg cagatggtca aaaggattcc ggagatcacc
    10321 acgctccagg aatagtccat gccgaagagc tgtacggtat gtgcattgtt cgcatcgaag
    10381 aaattatgta ccgcactggt gataccgttg atctgggtaa aggttccgat accgaacaat
    10441 cctacgcata cacccataaa agcgaagatc ttcgccagcc atttccagct cttgcccata
    10501 ccgttttcga tatagtagaa ggggccgccg ataatgtgtc catcctctgc taccacacga
    10561 tatttcaccg ccagcaggca ctcggaatac tttgtagccg tacctaccag cgcagcgatc
    10621 cacatccaga acaaagctcc gggaccgcct gctacaatag ccgttgccac accaacgata
    10681 ttaccggttc cgatggtagc ggacaatgcc gtacacagtg ccgcgaaact ggatacttcg
    10741 ccgtcttcgc ccgacttttc attggcaatt aaatgtttga ttgcatgggg aagcttcgtg
    10801 atctgcaaaa aacgaagtct ggctgtgaga aaaaggcccg tagccagaat gaggacgatc
    10861 agggggatgc cccagacgaa atcatccact gtggtcagaa agttactaat catgtctaac
    10921 atattccctc tccttccgtc acctcttgtg acgtcctatc gtagattgat ttcttccgaa
    10981 gagaatggga ataagcatat agatgctctg tccttttgcc tgagagattg gcagctttac
    11041 tgccgtacac cttcggcgcc ttctatacgg aaatatgaaa attgctttcc gtggaaggac
    11101 tctccagagt aaatctttac cgcgctaaag cggcaacaaa atcacaatag catacttttt
    11161 cacccattgc aacttttttt atctttagtc attgatctga ccaacacaga cattgacttt
    11221 tatggtacct gattactata atataattca tatgaatact aacacgaaca atcacaaaac
    11281 ttcatattcc attacacgtg gactgttttt cctgatcatg tgttctgtga tttttacggt
    11341 actgtactac agtctgccca ggcagaaccg tgagccggca atatcggatt atattgactt
    11401 ttcagaaggc tggaccacct ccgatggaag ccccgttgat ttttcccata tcagcggcac
    11461 ctgcacagtg accaagaagc ttcctgcgct gactcacgac atggcacttt tcttttttta
    11521 taaaagttcc aatgtgacga tcctgataga cgaccggctg atctatgaga ccatgcagcc
    11581 ggagagagtt ttcttcggca agacaccggg agcctcttat gtctctctgc cgatctaccg
    11641 ggaagattcc ggatgcacgc tcaccctggt gatcgataat ccttacggag acggcagtgg
    11701 caagatcaat catatgtacc tgggacgtag tgaagatatc ctgataagcc gcatccgcga
    11761 caaggcaccc ggattcggca tcagcttcct gatcgcccat cttggtctgg catttatcct
    11821 gttttatctg ccattacaca agaagcacat catcggcagt gagatgcttt atctgggatt
    11881 atttgctctg aataccggta tctttatgtt ggcagataac cgtatgctgc aactgatcct
    11941 gcgtaacagc catatttatc atacaattgc ggaacttttt atgatgctga tcaccattcc
    12001 actgtttctg taccttggca aaatgtatac agagtactcc cctgtgatgg tacagacagt
    12061 atgcctgatc agtgtcatgg atttcagtat acgtttctgc ctgaatctga ccggtctcaa
    12121 ggacttccat gaaagtcttc gtctgaccca tattactttt ggcattctga tcgcacttgt
    12181 tatctatgcc atcggcaagg ggttttatca gaatcaaagg cagcatctga agcacaatct
    12241 atatcataac cttggaattc tgtgtctgtg ctttggcgtt ctactggata tcctgctgct
    12301 atggttcggc agtgctcccg agaccagctt cttcacacgg atcggtgtgc ttctgttcct
    12361 gatcatggaa gcagtccagg ttaccctgcg cctcatgacc aactatcagg aaggggttcg
    12421 catgcagctg ctcagccgcc tggcttatcg cgacggtctg accgacctgc tgaaccgtac
    12481 ttcctatatg gaagaattga aaagactgga cgcatcccat aattttcatg tattgctggc
    12541 actgtacgat gtgaatagcc tcaaatatgt gaacgacacc tacggtcacc aaagcggtga
    12601 taaattgatc tgcagggtag cggatactct gtcggcacat ctcggttcct taggaaaatg
    12661 ttaccggatc ggcggagatg agttcgtatt cctgtcgacc gtatccgatt ctgaaaaaga
    12721 atttctcaag ctgcaaagag atttcgaagc gtctctggga actctgaagc ttcctgacgg
    12781 ttccgagcat cccattactg tagccatggg atattccgta ctgacccata atatgcctcg
    12841 ttccatggac gatatggtcc gggaggccga tgccaaaatg tatgaggcga aacgacgaat
    12901 gaaaacagaa aattgatcat aggatttgca gcgtttttgc tgacaatctt atgaatagcg
    12961 tggctcattg cagagaactg ccaacaaatg tggagtgccc tttgcgaaac atttatgaat
    13021 tggggacatg gctcgtaacg tagggttgcc atagccccca cgcagaaagg actgcccccc
    13081 cccggactcg gatgctagct acaactgtgg attttctaaa ggaaagtggt caagtatttc
    13141 tcatcatgac ggggccgcct ttacgctgcc atccatgtcg cttaccggct aacgcgaaca
    13201 tcgtgttcgc gttgcccctg cggcttaacc actttctaag aaaatctcac agtctccgcc
    13261 gcatctacgc ccgggggagc agtccttttc tgttggaaac cttccgcctc agcgtggaca
    13321 caggtgatag gaacaagctt ctctgtatcc ttaaaccaag gtatggggcg ttacgcacaa
    13381 tatttgataa ttaagccccg ctatcgtaag actgccgggg cttaattgac taaatttgat
    13441 gattacgccc cgc
//
LOCUS       DAWEJW010000079        13243 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1686_length_13243_cov_62.010843, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000079 DAWEJW010000000
VERSION     DAWEJW010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13243)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13243)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13243
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1686_length_13243_cov_62.010843"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2622..2721
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3311 a         3419 c         2450 g         3963 t
ORIGIN      
        1 cagcgctcga agcccagtcc gaagcctgca tgacgtgcgg agccgtagcg gcgcagatcc
       61 agatagaact gataatcttc cttattcagt cccagctctt ccatacgctt ctccaggatc
      121 tccagattgt cttcacgctg gctgccgcca atgatctcgc cgataccggg taccaggcag
      181 tccatagcag ccacagtctt gccatcctcg ttcaacttca tgtagaatgc cttgatctcc
      241 ttcggataat cggttacgaa tacaggtctc ttgaattcct tctcggtcag gtatctctca
      301 tgctcggtct gcagatcgca tccccagaat accttgtaat cgaactcatc gttgtgcttc
      361 tccaggatct tgatagcctc agtgtaggtc acatgaccaa aatccgagtt gaccacatgc
      421 tgcaatcttt cgatcagtcc cttatccacg aactgattga aaaatgccat ctcttcgggc
      481 gcattttcca gcacatagcg gatcacatat tttaacatgc tctctgccag gatcatatca
      541 tccttcagat ctgcaaaaca gatctccggc tcgatcatcc agaactctgc cgcatgacgg
      601 gtggtgttgg aattctcggc acggaaagtc ggtccgaaag tatatacgtt cttgaatgca
      661 aaagcataag tctctacatc cagctgtccg cttacggtca ggtttacagg cttaccgaag
      721 aagtcctggg tgtaatccac tttgccgtct tctgtcttag gtacattgtt catatccaga
      781 gtggtcacct ggaacatttc accggcacct tcacagtcac tgctggtgat cagaggagta
      841 tgcacataga caaatcctct ctccatgaag aaggtgtgga ttgcataagc gatcagggat
      901 ctgacacgga acactgcctg gaaagtattg gtacgtgctc tcagatgaga gatggtacgc
      961 agatactcaa aggagtgacg ctttttctgt aaaggataat ctgcagtaga aggtccttcc
     1021 acgatcactt ccgctgcctg catctcaaat gcctgctttg cctcgggggt agccacgatg
     1081 gtgccgcgga tcacaactgc cgcacctaca ttgatcttgc agatctcctg gaagttcgca
     1141 agtccgtcac catatactac ctgcaaaggc tcaaaaaagg ttccatcgtt gatcaccagg
     1201 aaaccgaagt tcttggagtc acgattgttc ctcacccagc cgcctacggt gatctccttg
     1261 ccgatataga cgtccttttc ccggtgaagt tctctgatgt ctgttaattc cataattttt
     1321 cctatccgca cgctgcgacg tgctctatct gaaccggccc gcatcctatt cgccggtcac
     1381 atactatttt taaaatttaa ttgttggtga tctttgcgtt ctctaccaga taatctgtca
     1441 ctttatcata gaggaaatac tctctgtagt cttccttgga ggtctcgccg atatactcct
     1501 cgatggtgtc ataaccggct gctgttgtgc ggtcctgaag catctgatcc agttcttcgt
     1561 cactgatgtt cagattctct ttgttcgcca ctgcctgcaa ggcaatatcc tgctttgctg
     1621 cctcctcaga ataggtagcc aggaaagatg ccagatcctg tccgtaataa tactgcgtga
     1681 acgtatctcc gtccacaccg taagcagatg ccatggaggt aatgctgctc tccgctgcat
     1741 cagaatattt ctgtaccaga gcctccggca cggaagtaaa ggtgttattt gccataaacg
     1801 catccatcac tgcctgctgt acattggttt cataattctg ctgtgcattc tcattcagat
     1861 aatcataagc gtactgacgg agttcttcct cgttcgtcac accaccgata ccgaagttac
     1921 tgatgacttc atcactgaat tccccatcct gtgcgggctg aatataattt accgttaccg
     1981 taaataccac agcctgtcct gccagatcgg attttccgta attctccggg aatgtaagat
     2041 tcagatccac tgtctctccg ggcatcacgc cgatcagtcc atcctcgaac ccggcaataa
     2101 agcttccgga accgatggtc agatcgtatc cctgtgcggt accgccgtca aatgccacgt
     2161 catccttttt gccttcgtaa tcgatgttta cggtgtctcc ggtctccacg gcacgatcca
     2221 taatcccgcc gttctctgcg gtaatatttt cataatatac attattgacc agactgtccc
     2281 actcatcctc atctacttcc acggtgtcta cggatacttc cagccccttg tactcgccca
     2341 gtgtcacata cttgtccaca tccatatcct tcaatgcagt actttctgct ccggcgccgg
     2401 tcgctgcatc cgtgccgtct cctgtatcct ttgctccgca gcccgccagc atacacatgc
     2461 ttaaaaccgc tgccagaaat cctaatgtct ttttcttcat tctaatactt cctatacctt
     2521 tcattttgtg cctaccatgc gaaatgcatc atagcaccgc ccattataac acatccaccg
     2581 ggctataaca agggtgacaa taaccggcag acctgctctt tnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn ntcatggatc tgctgagtcg tcttatccgg accactgctg
     2761 ataatctgca gatcacagaa atctctcgtc cctgcttcga ttccccggaa cagttccggt
     2821 tgtaaaaaca gattctccct cgctgcataa ttccagtcca gcacaaagcg cgcctggaga
     2881 ctgagtaccg cacttccacg gatcctcagg tgtgtatctc tccaatgccc gaatctttca
     2941 tccagatcaa tatattcctt ccctacattg aatccgccca cataggccac cttattgtca
     3001 atgactacga tcttgcggtg attacggtag ttgatacgca gctggagtct tcgcaggatc
     3061 gccgggaaaa actccgccgt ctgcacgccc ttttcattca gctttctcca aaagctgtgc
     3121 cgcacggaac gacaacccat ggcatcgaac agcactctca cttccacacc ctgcgccgct
     3181 ttttcgatca gaatatcctt gatctgattg aataatacgt cgtttttaat aatgtagtat
     3241 tggatatgga taaaatgctc cgcattccgc agatcttcca tcagcgcccg gaacttctca
     3301 ttgccgtcga tgaagatatc cacatcgttg tccttcgtca gcatggcttc tccggcctcc
     3361 agattataca tgaccagatc ctcatacccc cggatactgt catccatttc ctggacatcc
     3421 cgggatctca gccgatactc ctgctgtctt atatctttac tgatgtgatc ctccacctct
     3481 ttgatccgga acattttccg cttatgcata tcctgcccca gcagcagata gaacacgaac
     3541 cccagtaccg gtataaaata caacaaaagc agccatgccc acacactctt tggatcccgt
     3601 ctctgaaaaa acacgatcac gatggcaaac agcatattaa aaaataccag gtggttgcag
     3661 aaaaaatcta ttcctgtttt tacgatctca aatgcttcac tcataatact gccccttcag
     3721 atgaaaaaca tcccttttcc tcaatatatc ataatgcaaa ccttctttcc agcaaaagag
     3781 actgacaaat gccgaaaaaa tcaacattct cagtctcttc ttatcttttg tattcaagcc
     3841 ggcggtctcc ggccgtgata ttttatccct ctgtgatgag tgttctcttt ctatcctgat
     3901 cgcagaattc aatcacatag gaacaatcct gcagatctgc cttcagctgt gcagtacagc
     3961 cctgatgttc ccatacaaag cagattctgg atccttcact gcttactttg atccggcttt
     4021 cttccgagaa cgccgggcag gaattccgga atcgcagcaa ctccagttgc tgctttacaa
     4081 tcggcttact caaaccactg catacctgtg ctgtcgtcag atttgtccga ttgatctcct
     4141 tatgtcctcc gggacctgcc atcttcaccg cttcgtagtc gttctttccg gcgaacagat
     4201 ccagatacca gatctgcggt ttgcccggca taaatatctg caaggctctc gcaaggagca
     4261 tctttctctc atcttcgccc aatgcactga agtaggttgc attcacctga tagtaaatgt
     4321 ttttctgacc gtgaagatcc ttcacatatc ccccacggga gacaatagtg tcgatcagtt
     4381 tctgaacccg gtcctccgcc agaattcctt tgagatccaa cagtggaatc ccatcgtgac
     4441 agccaagcat attcacggtt ctgatatttt tatcgatcag ctcctgtgcc catccggcca
     4501 gatgttctcc gtttcccgat tccagggcat ctataatgag tcccggcaga aagaagtcat
     4561 aggtgacata tccctgctct gctatcttct catatatttt ttcactatag ctttcatgaa
     4621 tctccggcag aagttccata ccgtacggtt ccgcaatctg ttttattttg tccagcagct
     4681 cccaggtgtc cggctggttc aaaaaattcc gttcccccgg agtcttcggt gcataagcaa
     4741 atgcatccag tcttacaatt ttagcaccat atcccgccag tgtctttaac acttcacgat
     4801 agtactccca tacttttggc gactgaatat tcagatccat ctgtccgaga taataccgat
     4861 gctgctccat gtaattccag accgttataa cctgttcttt tgtcaacctt cctgcttttc
     4921 gctccagaac gattttctgt aaaatgtctg ccggcttttt tccttccggg cagcccatgg
     4981 atagttcctg tgcaataatt tccgcctgca tatattgcag atcggcagct ttcatcagtt
     5041 caggagcatt aacctcaggg tagttcactt cctgataaaa agtattccag taagggatct
     5101 ctctcccatc cggaaaacgt accataagaa tggggagccc aggcttccgg aaaaacatct
     5161 ttttcagata cttttcttcc ggaaggatat atccctgctc cgtcatttct ccgcagtcct
     5221 tccagaattc attccaatta atgaagaagt ccttgtactc cgattcttcc ccttttgcaa
     5281 tgatatcctg gaattgcttt gacaacaccg aagcatgatt cagaataaaa tccagcttca
     5341 attcaatccc gagctttttt attgcttcca atgtttcgcc ggaagcatac atcttgttca
     5401 gagaataatc aatcacagaa aagcctctgt ccagatccgt atggtacaga ctgggcagaa
     5461 tatagcagga agaaaatgct ccctctgcct cttccgtttt caaaaaatct acaacatcat
     5521 ccagggttcc gcccagacta tccgggtaga gattcaacat cggtcctatg ttcataacat
     5581 ctttcctttt tatttctgaa gcttcggaac aaattgcgca tattccttcc ggtctaacac
     5641 cgttccgtcc cgggtaacaa taagttttgc tttctcagcc tgctttttca gcattttctg
     5701 ctcaatatcc agtaccatca tcgtaaatgg ggtgtctatc cattgatcac ggtttctttc
     5761 cagacggctc tgcaatgtct cctggggggt actgtcaagg tagatggaaa tatccacacc
     5821 gctgtagtat tgactgttgc tgtgtgtcca ctccaccaac agcactttta ttccggtgaa
     5881 atccttctct tcataccaca gttctgtctc ttcgcgtccc atatgtctga cccatagctt
     5941 ctctccgccg gctttaaacg catgaaccag tctgtttact tcttcaaaat taatttcctt
     6001 ttccgtgcca agataatttt ccagagcttt tctgccgttt ctaagatagg actcatacca
     6061 tggatatttt tcgacatgtt tttcatccgc atcatttccg gctttttgaa attcctgaat
     6121 gattgtacat cgttcttctg tataagtctg ctctttcagc attcccacaa ttgcattttc
     6181 tctgaaaatc tgtagccgct ccgcatcatt gtacttggga attctatgcg gataagcatc
     6241 tccggacaaa atgtaatttt ctatcccttt ttccttcagg aagcaggaaa gtaacaaggc
     6301 cgtcactgtt tttccggatc cggagcctcc gcttactgca acgactactt tttcggaacg
     6361 tgtctgcttc gcacagtttt caatcatggg gagcagcttt tgcatcagtt tttctgccaa
     6421 cgtagtatca tatattttca gtagctcctt tgatgacggt tctgcttcac tttccattat
     6481 ggttcctttt tctatcatat ctgttccttt atcgtcatta ttttactttg tggactcccg
     6541 gtcgatcagt tctaccggaa atacaacctg ggatggaacc ttttcactct ttactttttt
     6601 cacaatcgct tctatggcat attcacacat ttcctgcacc ggctgatgaa tggtcgtaat
     6661 ggaaggatac gtcagtgtgg aaatccaggt atcatcgaat cccacaatag agatatcctc
     6721 cggaacacgt tttcccattt ttttgcatgc acggataata tttgcagcca ttacatcact
     6781 tgttgcaaaa attccatcca gatccgaatt ttttttcaat aattccaaaa tattgttgcc
     6841 gaattcttct gataagggag ccaacctgtc tgtataaacc acacgtccac tgattttttt
     6901 atcctcgcag cacttcataa atgccttttc tctgctatat gcagggatat tttccttggt
     6961 aggcgggtta aaaattgcga tatttctgca tccggcatca aataaccggt tagccgccaa
     7021 aataccaccc tgatagttat cgcaaagaac tgccggaatg ttttccgcaa tttctctctc
     7081 aaaagaaatc agcggacaat tggaatccag atgttccatc gtctctccga tatggctgca
     7141 cacaacaatt ccaagaactc tgtgtgaata gagcagatca atatactgta attctttttc
     7201 cctgctgttc tcggaaatac agaccattat cttgtagttg tgttttgtca cataatgctc
     7261 cagccagtga aggaccttgc taaagtaagg atgctctaca gatgggagaa tcaccccgat
     7321 ataatcactc ttctgccggg ataaatttct cgccatttcg tttggcatat agttcagctt
     7381 ttccatggca gaatatactt tttctctcgt ctcttcactg atgtatccac gattattcaa
     7441 tactctggat actgttgtgg aggtcactcc tgcttctgca gctacatctt tgattgttgg
     7501 catattccct tccccttatc ttactttttt gcatcagaca gtaacggaaa gtccgtcgta
     7561 agtcaacagg aacccctcct gacctaccag tctttcatat tcctgcggaa tcataccgca
     7621 catatgagaa taatgattca gataatattc cgtatccggt gtcactgcat gcattctgcg
     7681 gagtttctcc cgcagctcta cattttcccg aattcccata tgcgtgtcac catccagtat
     7741 acctctgctg gtatccatac tgacgacgtc aaattttatg ttctgttgtt ctataaaatc
     7801 cagtgtttcc tccggcaaag cccccgtatc atttgcatac aataaggtcg atcttccatc
     7861 ctcgatcaca aagatcagag cttcctcatc cagcttgtga tatgccttta aaggtgtaaa
     7921 ctttaaatga cctatacgga atggttcaaa cttttgaatc cggtgaaatc tcatccttgt
     7981 cagatctaca tgtggatcct tagctattat ctccataata ccatcccgaa ccgcatcatt
     8041 gccataaatt tccattactt ttttatcttt tttttgaatc acggatgcca tttcccgcag
     8101 tcggaagcat atttccgtgg tattcaggtg atcggaatga gaatgggtaa atactaggtg
     8161 atttacctcc gataaatcca catggctgtt aatacattgg gaaaaaatat ccggcgacag
     8221 gtcgatcatc acctgatcta tcagagtaca gctacgtgtc tttacatttt ttcccaacaa
     8281 cgttcttacc ttacgacatg cttcacactg gcagaaaata ctggggaatc cttctgctcc
     8341 gcctgttccg agatatctga tttccacttt atcacccctt aattccgctg gatgtcacag
     8401 atgccacaaa ctgcttctgg aataccagga ataatatcat cgtaggaata accattaatg
     8461 tagagaacgc aaagacctgt ccccaatcag tgtaggtatc ggaaaacagt gactttaaag
     8521 ccagcggcag tgttcggtaa gtaccgtctc tggtaaccag ggtgggccac agcagatcgc
     8581 tccaggaacg gatgaatgcc atgatcgcaa cagtggtaat gatcggctta gacaaaggca
     8641 tcatgatttt ggtaaatatt ttccagtagg aggctccatc aataattgca gcctcctgca
     8701 attccttcgg cattccctta aaatgattat agtatagata catgtacatg ggatatgcga
     8761 cacttggaat ggccagtcct atatatgtat ttaacattcc cagtgaattc actgtcataa
     8821 aacggttaat gataatggac tctgtaggaa taatgctcaa tgccacaatg gtggatataa
     8881 tgattgcctt tccacggaaa ttaagcattc ccagtgcata tccgatcatt gcattgattg
     8941 tggttgccag caggacaaag atgatcgtta ctacaacgga attcttgaag aacttgaaga
     9001 aatccgcttt cacaataaca ttgatatagt tatccagtga caggtctccg taaggaatga
     9061 atgctctgat atttgacata tccgattcaa tctgcgcata tggtttcagg gaagatacga
     9121 acaaaaatac gatcggtccg atgaaaatag ctgctatgag caaatatcca agatacatgc
     9181 acaatttcat aatgattttt ttcctttttg acattgcagt tctcattccc tagtcctcct
     9241 ttgcaaactt attctgcaga attgaaataa ccattaccag cataaagaat gtaactgcga
     9301 ttgcggaaga gtatcctacc tgtccattta cgaatccggc tttatacaac tggaatacta
     9361 cggtcgttgt tgcattggac ggtccgccgt ttgtcagtac atacacctgg gtgaatactt
     9421 tcagggaatt gataatcaca gataccaaaa cgtataccag tgtattcttc aatccgggta
     9481 ccgtaatata aataaatttc tcaaaggtgg tacatccatc aatttcggca gcctcatata
     9541 gttcttcgga aatgtactgc agaccactta aaatgatcaa catctgcata ccaagattct
     9601 gccagatata cataattgcc agacacggaa gtgccagatg ctgatcctga agccatttct
     9661 gaggtgcaat gtgcagcaga cccagaaacg aatttaataa tccgctggat ttggaggaaa
     9721 aaatgaatgc ccagataact gccactacag tcatcgttac aacctgcgga ctgaaataga
     9781 tcagtcggaa cacctgtacg cctttgatct tctggtttac aaaaaccgca agcacggttc
     9841 cgatcaccaa taccgaaggc actacgatca gtgcatacaa aaatgtattt ttaaaagaaa
     9901 gtactgtcac tttgcttgtg aaaatctcaa tataattctt caaccctaca aaggctgttt
     9961 ccattcccac tttgggaatc agttgtttat tggtaaatga atacacaaca gacattgcaa
    10021 acggtaaaat gataaatacc agtgccagca ccactgccgg tgtgcagaac aggaatgcgg
    10081 cttttgactc gccacgttgt aatccgcttt ttttcttaga actgtttgcc ataagtactc
    10141 ctttttttat gaataaggct gccctcacag acagccttat ccgatacttc ttatttcaga
    10201 ttattgaaag ttccatccgt tctctgtaat aatttcatca atattcgcag cggcatcgtg
    10261 caactctgtt gcaggatctg cgccggcata gatattaccg ataacgctgg ccattgcatc
    10321 ataaatggtc atatgtgcag gggtgtacgg acgcaggaag gaaataccac tctccagctg
    10381 ctgtctgtac agatacaggg tacctccctc acaatagtcg gggattgcat ccataacact
    10441 ggatcttgcg ggaaccgcac cgttgacatc tactaccatc ttgatgtaat cggggttggt
    10501 tgcttcctgc agcattgccc atacagcatc atcctttccg gattcctgtg ctcctgtggt
    10561 catgatcata acagtagaac cggagcaggt gtaaaccccg tgtcccatat caggcagggg
    10621 aaccaggatc agatcatcgc caagtgcagc cttataatcc tgatacttgg aatgtccata
    10681 cagagccagg gcattctcac ccttctggaa agaatcttca taatcacagg ttgcatctgc
    10741 atatccatct gtgaacagct ttgccaggaa atcgaaagaa gcaatggtat tatcgccgtc
    10801 cagagttcct gtgcaaagtc cggtctcacg attcatgtag tctccgccaa agctcgctac
    10861 aaacggcatc caggtgtaat actcggtgga aggtttgttc tgtcttacat agatgggcca
    10921 ttctacaccg ctgtctttta atttcttcag cgcatcttca aactctgctt tatcccatgc
    10981 atccttgtaa tcggcaggaa ttctgacacc ggcattctcc agcatggatt tgttagccca
    11041 gaaagccagg ccactatcga actgtgcaac ggaaatgatg tgatcattat aagtgcactc
    11101 agatactacg ctgggaagat aatcatccat cagttcatcg gttacataat cgtccagatt
    11161 tgctaccaga cccgcttcgt agtatttggg aatcaacaga taatcggcaa ctacagcatc
    11221 cggcatgtcg cctgcaatag aagaagtctg catcttattg tccaattcat ccttggaata
    11281 atactcatct gtaatggtaa tgttcagatc tccttcgatc ttgtcgaaca tttcacggta
    11341 tatatcatct tctgcgccgg agctgatcca gactttcact tctttccctt cataaccgcc
    11401 ctgtgcatct gttgcagcga ctgctgttcc atccgcagta tttgccgttg tttcactctt
    11461 ggactctgta gctgtggcac ctgtctctga gcttccacaa gctgctaatg atacgaccat
    11521 cattgcactc aacaatcctg ctaaaatttt ctttttcatg tattgttctc ctctctgaat
    11581 ctatacattt tgtagatatg taattgtatg tcaaccggtt acatattttg taattaagaa
    11641 tatatactaa tttttatcgt gtgtcaaccg gttacatata taaattttca acaaacttgg
    11701 atttgatttt ttgtatagtt tcactttgta ctccaatttg acgcagatat tccggaactc
    11761 caccccattc ctggtcaatc cagttcagaa tccatttcat gttgtagata ttggaaccgg
    11821 aaatatcggt cgtaaatttt gtaatatagg tactgctaca ttggtaatcc accagaatat
    11881 cttccctctc cactcccacg gatgataaaa tcagcattgc cagaatgccg gtgcgatcct
    11941 tgccggctgc gcaatggaac aataccgctc cctcggaatc agcgactacc tccagagctt
    12001 tcctgatctc ttttcctccg ttgcgaagca tcaggcgata catgcgatgc agcgttttgg
    12061 tctccaccac actactgttt acaacatcca gctgttccag ctttgtggta cccataagcg
    12121 aacagttgat atagtccata tcctgtccca gtctgtccgg cagttctttt gtctcactgg
    12181 gaaatcttaa gtcaatgacc gtcctgacgt caagatgctc aatcttcgca atatcttctt
    12241 ccgtcgcctt atttaaggct gtactccgga aaatcacacc atatctggtt acaccaccgt
    12301 cacacgcata gcctcccaga tctctgaaat ttgccacctg atccagacga tatctcctac
    12361 aattcatctc tttccatccc ttttgatctc tgttattgtt attttattcc ttatcccaac
    12421 ggttctctcg atgtctctgc atccccaaaa aagaacataa aatcctgaat ttctgttctc
    12481 atcattttat cggtacttat aaattccttt gccttttcaa actgtggttt cctgtcgtag
    12541 gaaatttgtt tcaatcggat ctcgccgtct gtaatcgtcg cataatgtgc cgtcttatcc
    12601 gggtcatcgg gtcttcgata cgaggcactt cccgggttca gccattccat tccatcaccc
    12661 agaatatgaa tacattgtct gtgtgtatgc ccgaaaatca gtcttctgtt ccctgtatta
    12721 tctgttccgt aatgctccgc ccaatattta tcaaacgcgt acctgctttc tatcgtccca
    12781 tagccactat catactgatg ggcaattccg tatttccatc cgtctgcctc aaaatatgca
    12841 gtttccggta actgcttcag ccacctaata ccctggtcgt ttagttgttc acaattatag
    12901 tgaacccact tgtattttcc atcttctaca tttttccatt ccttactttc ataagtattt
    12961 ataacgtgaa gatcatggtt gccatacaca agatgcgtgt ttttcagatt gcggaaccgt
    13021 tctatcactt ccgccggata gattccatag tctgtataat ccccggcaca gcacagaata
    13081 tcgtaatcag cctctttttc caaaatggtg ttcaatgcgg ctatgttcgc atgtacatct
    13141 gatactacca gtactttcat tttccgcacc tccttctttt ttcacattat ataattttac
    13201 cgctcgtatg tcaaccggtt gacatacgtg tttcataatt ttt
//
LOCUS       DAWEJW010000080        13179 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1695_length_13179_cov_58.023926, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000080 DAWEJW010000000
VERSION     DAWEJW010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13179)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13179)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13179
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1695_length_13179_cov_58.023926"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9247..9346
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3082 a         3593 c         2710 g         3694 t
ORIGIN      
        1 ccccgaccca cggcttagaa ggccgttgct ctatccagct gagctacgaa cacatatctg
       61 tctgttagct acatcagcga accgacgaag catattgtaa cttatccgac atcatttgtc
      121 aacagttttg tgctaatttt ttgaaaaata aatacaaaat ccaagttaca cgtcggggtg
      181 acaggattcg aacctgcgac cccctggtcc caaaccaggt gctctagcca agctgagcca
      241 caccccgaag ctgcgttatc gcggatttcc gcaacgcaga tgttattata acccagagat
      301 tttctatctg tcaagagaga agtttgaagc atcactggcg aagataagca atcgtaaaat
      361 gcacctttcc ctctctgtca atctccatga tcgcatagga aggctggcgt ccctcctgtc
      421 tcggatagga cagacttccc gggttcacgg caatgatgtc atcatcaata tcgatcaccg
      481 gccgatgggt atgtccgtac agcacgatat caacgccccg gtcaatggcc tcttttttga
      541 tggtctcatt tcccatggat acataataat tgtgtccgtg ggtcacccat actttgtagg
      601 atccgatatc cagtgtgatc tctctgggga gatccgagaa aaagtcattg ttgcccagga
      661 cgatataaac cgggcattct gccgcagaag aaagtgcata ctcactgccc tccgcatctc
      721 cgcagtggat caccatgtcc gggtggtgca tctccagtac tttgaagtaa ttctcattct
      781 ttttatgggt atcacttacg atcagtatct tcatatttct tcagttcctc tttcatcagc
      841 tccaatgcct tacctctgtg actgacctgg ttcttctctt cttctgtcaa ctccgccgta
      901 ctcttgccga attccggtac gtaaaaaata ggatcgtaac cgaatccgtt ggtccccttc
      961 tcttcatatc cgatccgccc ttcgatggta gctcttgtgg taagttctct gccgtccggg
     1021 agcactgctg caatggcaca gacgaatctg gcagtacgct tttcctccgg cactccttcc
     1081 agtctctgga tcagatttgc attttttatg gaatacggcg tatcctctcc cagatatctg
     1141 gcagaataga ttccaggttc cttattcagg gcatcgatct ccagtccgga gtcatccgcc
     1201 atcacgatat ctttcgtaaa agcagccacc gcacgggcct tgatcagtgc attctcttcg
     1261 taggtcttac cgttttcttc aatatctgct ttaatgcccg catctttcat ggatacgact
     1321 tccagaccgg tatcctccat gatcatgcgg atttctttga tttttccggc attgccggtc
     1381 gcgaatacga ttcttccctg catattaact ccttgccgca tttgtgttct tatatagtca
     1441 tcattgcgta ttagtataaa cctcggaaat agcttctgca ataccttccg ccagcttttt
     1501 ttgatagctt tcctgttcca gcagctgtct ctcgctgctg ttggtcacat atccgagaga
     1561 aattctggct gccgggatct tcagctgcca cagaatgtca tcttcctccg ccgggaacaa
     1621 tccaatggcc cggttgctgg aagccaccgt cacttgtctg gtcacgcagt cggcccattg
     1681 tacatttccg aaatccggca gataatactc atcattgtat tctgcccgga tgccgtaggt
     1741 actctcctgt gcatcatctg ctgacagtcc gatctccaga tacagatctg catccaccca
     1801 gtcagcgaag ctacgcctta cttcctgtgc gacctctgta tcttctgtac gggtaagata
     1861 tatttttacc atgcttcctt ccaacagctg tgccgtctgt cttgccacct ccagagcgat
     1921 gttcttctcc tcacatccgg aatcggcaac gcctctgtcc cttccgcctc ccacgggatc
     1981 cagcactacg atccggtcgt aagattctct gggattgaca aaatcgatct tcagagttcc
     2041 ctcttccaga gtactgtgat actccaggat ctccttcatg gacaatctga gcaatacgcc
     2101 ctcgttctgt gcttcacaga tgccgtttcc caccaggctg aaatctccgg taagctgatg
     2161 ttcccggtaa aaagattttc tgccgttctg cacatagatc cagagttccc tttccatgta
     2221 atgattttcc accactacat tttccgcttt cgtaccggtt tccaatggta tacagatctc
     2281 ccgttctgcc ttcctgtctt ccgccatggc cagctgcatt tcatgggacc gttccgcctg
     2341 attctctgtg accagaccgc cctgttcctg acttaacgca gagatcacca ccgtcttatg
     2401 cgctgccgtc cagagcatga tgcccataga catacttccc agcaggagcc agaggatcaa
     2461 tgtaatgcgt ctattctgtt gttgatccct gatctgcagt aaatttttca tttcttctaa
     2521 acgtctcttc tcttcggagg cttcggtccc agaaactgat aataataagt cttcatcatt
     2581 ccgttgtaga tcttgcggtt cttgtccgcc ttcctgccga tatccttctc tgcctgctca
     2641 taggcagtga tcagatacat actccaggaa tccagttccc ggaatcgttc tccgatggta
     2701 cggtacaact gcggcatctg actcttatcc tgcaaacgct ctccataggg aggattggtg
     2761 ataataaagc cgtatttctt cgggtgatga agatcttcca caccacgacg ctggaaatgg
     2821 atcagcttgt ccacacccgc catccgtgca ttttctctgg cgatcttcac catatcctcg
     2881 tcaatatcat agccctgaat atctgtctcc actgacagat tgatcatctc ttccgcctca
     2941 tccattgtat cataccatac cttttttcct atgatatggg gccattcttc cgccgtaaaa
     3001 ctgcggttcc tgccgggtgc catattggcc gccatcatgg ctgcctcaat agggaaggtg
     3061 ccgcttccgc agaagggatc caccaggatc cttcccgcat tccagggagt cagttcgatc
     3121 agtgcagcag ccagattctc cgcaatgggg gcttttgccg tcagcttacg atatcctctc
     3181 ttatgtaagg actctccggt gctgtccagt cccacggtca cttcatcctt catcaggaat
     3241 acccggacag ggaaattttc tccatcttca gggaaccagc tgatctcata ctgggccttc
     3301 agccgctcca ccattgcttt tttcataatg gactgaatat ccgaggggct gaacaatttg
     3361 gactttacac tagctgcctt ggccacccag aatttgccgt ccttcggaat gtattcttcc
     3421 cagggaagat ttttcgtgcc ctgaaacagt tcttcaaaag tctccgcatg aaaacttccg
     3481 attttgataa ggattctttc tgccgtccgc agaaaaatat ttgctctgca caaagcctcc
     3541 tcatctccga aaaaggtgac acgaccatcc gccacttctg taatatcata tcccagatct
     3601 gtgatctctc tttttaatac tgattccata ccgaaatggc agggagcaat caattcaaaa
     3661 cgtctcattc gttcctccgg atcttagtca tcaattattg cacatataaa gttattatat
     3721 atggattctg aaaatatgta aatagggctg ccttgcggca gcccctgaaa aattatgcac
     3781 ccgattagca gctcttgttc tgctccctgt tgttgctctg attcttattc tgattctggc
     3841 tgttctgctt attctgggag ttcttactgt tctcgctctc gtcataagag ttgttatact
     3901 tgttctgatt gttctgttca taattgttag acatatttgt gacctcctga taggaaatat
     3961 cttttttagt tacaggagta gtatgtctat ttcgcacaaa aatatgccca aaaattatgg
     4021 tactttagaa ctaaatttct tcttgcattt ttatatcatt agtactataa tcataattgt
     4081 aaatagaaac cccttcatga ttctatttgc acccttatgt attatttatt tatactcccc
     4141 tcatgagagc caccagcttc cccctggcgg ctctttacgt tggaactatt cagagtcaat
     4201 aaatataatc ggagcaaccg tcagatgctc gcatctgtaa ggtactccga ttatatttat
     4261 ttgacgaagt aacctcaatg agcaaaaagt aagcctcgca gaggcgtttt gcgaattgag
     4321 ggactatgaa tagctgattt atatagttgc tagttaagcc agtcccgaaa taatttcacc
     4381 aggaacaata ctaccacaat cggaatcagt accggtgcca gaaaagacaa tacggaaaat
     4441 accgcaccga agatcaatac taccaccaga atcccaagga tcagccacac ccagcccgga
     4501 agcttcgcca gaacatttcc caccttactc tggtgtgttc cgtagccatc gtcttcctct
     4561 gtataactac gatggacatt ctgtccggca gcctcccgat actctccgtt gccgtagacc
     4621 gtgttccggt atccggaacg gttgtctcca ccgttggtct gaatgattgt gcgggcgatc
     4681 agtctgggat ctcccaggct ctctaagact tcctcttctg tcctgccctt gcggatctct
     4741 gtatttatat aatcctggta ataatttaca ttatccatga ccagaccggg agaaacacgc
     4801 ccattcagcg ccatccgcag tcggtccaca aattcctgtt tgttcatact ttcacgactt
     4861 cctccattta catcatcatt ttagcatgac ataagtacca cgtaaatata cggatcggga
     4921 agtatttcta aaacttttct aaaatgcaaa ataaaaaccc cggagacatc atctccgggg
     4981 ccttagatgt acgtgacagg attcgaaccc acgaccttct ggtccgtagc cagacgctct
     5041 atccagctga gctacacgta catttcattt acaggttttc gcctgcaaca gtatgtattg
     5101 taactgctgc gggcgaaaat gtcaatagaa tttttcaatt ttttcaaaca attttatctg
     5161 ctgcctgctt ttccgtagcc tgtacgactt ttacgtcgta tttttccagg atggcctgct
     5221 tgaattcttc gtaattgtct tcatcatcat agcaggcata ttcgatctcc agcggttcct
     5281 cagtcttcat caggagcatt cccaaaagtg actttgcatc cattgccatc ttctgaatac
     5341 ggatgtcgat tgcatcgtcg aattcgttgc aggtatctac aaaatgtctg atatcatcct
     5401 ccgagtggaa ggtaatatat aactcttctt tcatcctctg ttctcccatc tgtccgcttg
     5461 tggacacccc atacatttat cactattcag agccaaaagt aaagtctcaa agagacgatt
     5521 ttgcgaatga gactctgaat agtagctgtt ttctgcctag cgtgctgcca gcgcctgtgc
     5581 gatatcttca cgcatatcca gtactgccgc acgggaagca tctgcataat tctgctcgcc
     5641 aaactgtgcg tatttctcct gttggtatgc ggcaatgatg ccacgggaag agttgataat
     5701 ggcacccaat ctgtcctcat tgaaaaaatg taccagatct gcgcccttac cgccctgtgc
     5761 accgtaaccg ggcaccagaa taaatgcctt gggcattatc ttacgcagta ctttgccctg
     5821 ctcaggatag gttgctccta cgacagcacc tacatagctg taggaatcac ccatacagtc
     5881 agctccccag gctgctacct tttctcctac cagctcatac agaggtctgc cgttcaccag
     5941 ctgatcctgg aactcgccgc tgctgggatt ggaagtcttc accagtacga aaataccttt
     6001 tttctcttcc ttacagatct tcacaaaagg atttacaccg tcggatccca gataagggtt
     6061 cacggttaca aaatcttcat caaaaccgta atagctcttg ctgcctacct gtactttgcc
     6121 cagatgtcct accgcatatg cttcggaagt ggatccgata tcgccgcgct tgatatcgcc
     6181 gatgacgatc aggccttttt gcttacaata atccacagtc ttcttaaatg ccaccatgcc
     6241 gggaataccg aactgctcat acattgccac ctgaggcttc acagcgggta ctaaatcata
     6301 aatagcatcc acaatcccct tattgtactg ccagatggcc tctgcagcac cttccagtgt
     6361 ctcaccgtat tcggtgaatg ctgctttctt aatatattcc ggcacgaatt tcatcatagg
     6421 atccagtcct accacgatcg gtgcgttggt ttctttgatt cgagtgatca gtttgttgat
     6481 catatttcct ccgttctgca atagatctgc ttttgaattt atattattac acagcaaagg
     6541 caccgccttc gggcgatgcc tcctgacctg tttctattct accccaacta atgtcagttg
     6601 gtcaacgtac ttcttgatat ttgaagcatt tacatacttt ttatggctgt caccgtttac
     6661 caccaccaga gtcggtgcct gcattacacc gtaacgtctt gccagctcca tattctcctc
     6721 cgcatcgatg gtcacgtagg gtacattttt cagatattcc ttcaccagct tacagttagg
     6781 acaggtcttg gtggtgaaca gatacttgat gttctgcggc tgctccactt ttacatccac
     6841 ctcttctgca aaattggaca aggtcaccac gcttctggtg ggacgcttca gggaagaatg
     6901 tgcaatatca tactctgttc tgttggcgta ttcctgtagc ttaccatcat tccagttctg
     6961 taccggacga tagtaaccgg tgatacggct gtagacctcg gtcttcgccc cacagtgagg
     7021 acataccttc acttcgcccg ccagataacc gtgttcctta cagatggaat aggtcggcga
     7081 cagcgtataa tatggcaact tgtaattgga agcgatcgtc cgcaccaggg aagccgctgc
     7141 tttccagtcg ggaagcttct ctcccaggaa tgcatggaac actgtgccgg aagtatacag
     7201 ggtctgcaat tcatcctgaa tatccagtgc atcgaaaata tccacggtat agtccaccgg
     7261 cagatgggag gaattcgtat aataaggggt atctccgggc tttccggccg tcttgatgcc
     7321 gggccagcgc tttctgtcgt gctttgccag acggtacgtg gtactctccg caggggtcgc
     7381 ctccagattg tagaggtctc cgtactgctc ctgataatcc gacagacgct ctctcatatg
     7441 gttcagtact tccttggtga actcctgggt cctgggatcg ctcatatccg ctctcagcca
     7501 gttggcattt aagcccactt cattcatacc gatcagaccg atggtggaaa aatgattctc
     7561 aaaggttccc agataccgct tggtataggg atacagtccc tcgttcagca gcttcgtaat
     7621 gacaccgcgc ttgatcttca gggatctggc tgcaatgtcc atcatgtggt tcagtcttgc
     7681 gtaaaactcg tccttgttgg cagacagata tgcgattctg ggcatattga tggtgacaac
     7741 gcccacgctt ccggtgctct cgccggaacc gaaaaatcct ccggtcttct tgcgcagttc
     7801 ccgcagatcc agacgcagtc ggcagcacat actgcggaca tcactgggct gcatgtcaga
     7861 attgatataa ttggaaaaat acggtgtacc gtattttgcg gtcatctcga ataacagtct
     7921 gttgttctcg gtgtcggacc agtcaaaatc ctttgtgatg gaataagtcg ggatcggata
     7981 ctggaaacca cggccgttgg aatcgccctc gatcatggtc tcgataaacg ccttgttgac
     8041 catgtccatt tcctttttac aatccttata tttgaaatcc atctccacac cgcctaccag
     8101 cgccggcagc tctgccagat catcgggcac cgtccagtct agcgtgatat tggaaaaagg
     8161 agcctgtgtg ccccagcggc tgggggtgtt gacaccgtaa ataaatgctt cgatgcattt
     8221 tttcacttcg ggatagctta aattatctac ctttacaaat ggagcaagat aggtatcaaa
     8281 ggaagaaaat gcctgtgctc ccgcccattc gttctgcatg atccccagga aatttaccat
     8341 ctggttgcat agcacggata agtgtctcgc cggagcagaa gtaatcttgc cggtgatgcc
     8401 gcccagtcct tccttgatca gctgctttaa ggaccatccg gcacagtagc cggtcagcat
     8461 ggacagatca tggatatgga tatccgcatt ccgatgtgcc tctgcaatct cttcatcata
     8521 gatttcagac agccagtagt tcgccgtcac cgcacccgag ttgcttaaga tcagaccacc
     8581 tacggaatag gtcaccgtgg aattctcttt cacacgccag tcttccacct tgacataact
     8641 gttgaccaca tccttgtagt ccagaatggt agacttcatg gcacgcattt tctcccgctg
     8701 cttgcggtac agaatatatg ctttcgccac ctcttcatag ccggcctgtc ccagtacacg
     8761 ctccacactg tcctgaatgt cttccacgtg gatctcgtca ttctccacct ttttctggaa
     8821 atccgcagtt acccgtaacg caagaagatc aataatgtca ttattgtagc tcatctgcgt
     8881 cgcagtaaaa gccttcataa tggcatcatt gatcttcgtc agtgtaaagt ccgctttgga
     8941 accatctcgt ttgattacct gaaacatctt atgtaccccc ttaccttcgt gtccccatgg
     9001 gctttcgccc gtaaaaccta tcacggtcta ttctacaata ctttgtaggg gaagtcaatc
     9061 aaaggataca agatattgtg tatacatcag ttcacccgtc gatttgtgcg gagacggctt
     9121 tacgcttgcg taaggagccg gctctgtgca aatcgacgag gggagcgcca tctcatgagc
     9181 aaaaggcagc tgcgtagcag cggaaagcct cgaagaggcg attttgcgaa tggcgcgggg
     9241 tgaacgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttac gctctcttcc
     9361 acaccctgtg gtacaacaca tacgccacca ccgcagtcac gctctccgtg cagacaaatg
     9421 cccaccacac accggtgacg ccgatcaggc ggctcagcac aaatgccgca gggatgatca
     9481 ccgccacata ccgcatcaca gagatcacca gggaagccat gccctgtccc agcgcctcca
     9541 gtgcgccgga gctggtcacc gacacagcgg acagaataaa gcccagactg atgatgtgca
     9601 gtgctgtaat accgatggta acagtctccg gattctcggt gaacagtccg atcagtcctc
     9661 cggggatcag ccagcacaat gccgtgccga tagccatgat cccggcagtc agttccagcg
     9721 tcaaccggta gatctgctcc acacgcttcc gctctccggc accgtaattg taaccaatca
     9781 gtggccggat gccctgaatg atgccattgg agggcagata gataaaggtc tgcagcttgt
     9841 aataaacgcc cagtaccagc acatacgcac ccgaataagc cgccagaata ccgttcagtg
     9901 cggaaatcaa cagagacggt agtgccatat tcaacgtagc cggaatgccg atgccgtagg
     9961 tcttgccaca gatctctcct tccggtcgca gataagttct gcgcaatttt accggcaacg
    10021 gatccaacac gtaatagatg atatatacta ataacgtgat cacctgaccg attccggtag
    10081 ccaaagccgc tccggcgatc tccatccggg gaaacggtcc gataccgaag atcaacaacg
    10141 gatccaacac aatattcgtc acaaatccgg cgatcatact gaccatggtg gcacgcatcc
    10201 ggcccaccgc ctgaaagatt ttctctaacg tgatacctcc ggtgacaatg gtggaaaaca
    10261 ggaataccac attggaatat tccatgccca gtgcccgcac ggtatcgctc ttcgtaaaca
    10321 tccctaagaa ccagttcatg ccgcagagga ataagatcat aagcagtacg ccgtgcaata
    10381 ctcccagcac catgccctgg gttgccgtct tatccgcttt ttcctgattc tgtgctccca
    10441 gatagaagga gatcatggca ttcacaccca caccgaagcc cactgccacc gccgtcaaca
    10501 agttctgtgc cggatagact aaagacaatg ccgtcatggc atcttcactg agcttcgcca
    10561 cgaaataact gtctacaatg ttatagaggg aattaaatgc catggacagc atcatgggaa
    10621 gtgccatgga catgaccaac gggaatatct tcttttcttt catataagtc tggttcattt
    10681 tttctctcct taaacaacaa acgccacaca acattcctgc tgtgtggcga aaataagatc
    10741 gatatcttga aaaatccact cccttacggg ttttatgacc ggttattcat tttgttccag
    10801 agcgtattat agggaagaaa tgcctttctg tcaagcctgt acgcccagta tttttgcaaa
    10861 taactgtgcc ccttcctccg cctgatagat attgataccg tcaatgtctg tgctgtggtt
    10921 ggcaaaggca gggtacaaaa atcccgcctc cggttttccg gcctcgtagt ctccggtcac
    10981 tggactgatc gtgccgatga gaaactgata gacttcctcg gactcccact ggttctcttt
    11041 gtcttttccc ttggtgggta catcgtagct gccgaagaaa agataaacgg cataaggata
    11101 tcccggctgg tatctctccc caatatattc atagagggag tacaataatg catcattttt
    11161 cagttcgcac tgccgtaatg cctccaataa ctgcataatg ctgcccggtt tccgggctcc
    11221 ctgatccagt cggtaatttc tcagattcac attggtattg gaataaggta ccgtcttggc
    11281 aatattcaga tttttctctt tttcgcttcc ggacaacttc agaaaatgag tattaaaagt
    11341 accgtcaata aacccttctt cgtccatata ggctcctgca attctggaaa aacagttgcg
    11401 ggacacggtc attcttctgg tcagttccag catatcttct ctgtctatgt gcattcttaa
    11461 cctctcacct ttcagcagtc tgcaagctct gtatccggaa cttacagcag tttgctcttc
    11521 gtatttacac tttcactgtc ctgatattcc aacagagaca gctttccttc ccggcattcc
    11581 agaatcgcag tagcacagtt gcccatatgg aaatgccaga agtcgctgac cggaatatcc
    11641 aacaccgcat tcaggatact gcgattcaat gctccatgag ctaccaccag cactgtttca
    11701 taagttccct ccaacggcag caacacctgc ttcacgaact cgccgcttct ggcatacaac
    11761 tgggcaaaac tttccgctcc ctcgggcggg agatagctcc ccggatccgt aaacggactg
    11821 ttggccccct cccggatttt acggacatcg gtcccttccc atggtccgaa attgagttcc
    11881 cggagtcttt cgtctgtggt cagcgtaatt ttcctctcgc ccacaatggt ctctgccgta
    11941 tgtcttgcac ggatcagcgg ggaacaaaac gcgatttcaa aaggaacttc ctttaatctc
    12001 tctgccgctc ccgccgcctg cgcaaaacct tgagcatcca ggtcaatatc cgtctgtccc
    12061 tgcagccgcc ccgcctggtt ccagtctgtc tccccgtgtc ttaccaaata gattttcata
    12121 tatcggtatc ctcattcttc tgtcgtaact tacttttctt ctgcctacat ttttcactta
    12181 ccctgttatc cacagtggtc tgtttcaaaa tactacggtt tccaccgttc tgtcaacctg
    12241 ctccccggac gtatacgcaa aaacagtggg ttccgctccc ggttcccgct gtctttttca
    12301 taattgccta tatcgatttt cttatttttc gatctccgcc gtcataatgg actttgcctt
    12361 tgatgtcagt tccgcacagt actctcccag ccgcagatac accggaatct cttcctccgt
    12421 ccggtttaaa agcaccaccg tgatccgccc gtccttttca aacgccgtca cctcgatctt
    12481 atctgtgtat ctggtaacac cgatccgctg tgctcccacc tttaggaaat gtgtaaaatg
    12541 ccacaggtac ccgcggctgc ggtattcctc aatgtaacgg cagatatact ctgcccagcg
    12601 cttccggtat tccggcttta atttgccgcc gttgttccgc tctccattgg tcttcatgta
    12661 ggccggcgga ctccaggggg gacaggagga tatccagctt cccgccgtaa gctttctccg
    12721 ccgcatccag cagtggcaga atgtattttt ccactctttc aaaagaaaag tgttcaaaat
    12781 tctcatccgc ctcttccccg tctgcttcgt aatgcgccag ggaaaaatcg cagctgtcta
    12841 taggaatccg gacacgatag gtctctgttt tggcatctgt cgttgcagcg cctgttcctg
    12901 ttgcttcatt tgttccgctc tgtccacatc cggtcagcct ggatactgcc atggttaccg
    12961 caagtacggt agataataat ttacgcttca tacactcgtc ctcccaatag gtaaatattg
    13021 ttttttgtat ttatcattat cactgacggc cgtctgtttt atttgataat tgaattctac
    13081 cattagtttc catcaggcaa cagatagcaa attttcaaaa agggggattt ttttcgaatg
    13141 gaaaaaggtg gataatatta cactgtaaat ggaaagtta
//
LOCUS       DAWEJW010000081        13094 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1712_length_13094_cov_64.800215, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000081 DAWEJW010000000
VERSION     DAWEJW010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13094)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13094)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13094
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1712_length_13094_cov_64.800215"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5623..5722
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7673..7772
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3217 a         3671 c         2644 g         3362 t
ORIGIN      
        1 ggcacataca ggcagatgga accgcccaga ttaccgtcac tgttgttgct tgcttttgag
       61 gttccgctga tctccatcgt accgccttcc acatatccct ggaactttcg cattccgtcg
      121 gcttctaccc ggaagcagtt gtcctctgac acccgaatct tcatgacaca gccaccggct
      181 tgcacctgta gatccgtgat gccctggctg tcatccccca gaacatacag ctctatcttt
      241 cccgacttta cttcataatc tgagtcataa tccaccttgt cctccagatc atagtgcaca
      301 tcgcccatat tttcccggat ctgctccgtc acaccgtcac tccagtcatc taccttttct
      361 tcggagattc ttcccagggt aaccgctgac aggatctccc ccggcgaaaa atgcgttgag
      421 ccattcccca ggcttccgct cactcccagg atcagtccca ataatataaa tatcaacgct
      481 gtgatggcac agcccttcat aaattttctc atgcttctgc tttacctctt ttctggaata
      541 atcctccgat ccaacaacag atcgccggtg tggcgattcc cgccatggcc acggtcaaca
      601 tcaggaacag aagtccgaca cctccgcaga tcagtccggc accgatcagc cccattccca
      661 cgaagggatg cgtaaacaga ctgatgatcc ctgcaaccac cagtgcgatc agaatcagta
      721 tcagtgctgc tgccgttgcc ccgaaaccaa cgatcagacc gagccatgca gcgatcagcc
      781 ccgctgccac accgatgcct gcggaagcta ctcccaggat caccggagat gccagaatac
      841 acaaaatcac aattaatacg atcatgcccg tggacattcc ttcttttttc cgagaggaca
      901 ctgctccagc tgcctgtgct tcattttcct gcccgaagcc tgcctctccg gcaccgctct
      961 ccgcgccgga atccgccgga taagtgatca ccgcacggtt gtccgcattg atcttctggt
     1021 aaatattctc tccataaccg ttcccgaaaa tgtccttttt gatgttttcc gccaccttga
     1081 caggatttcc caaagcctca ataacgttct gttcattttc cggacccgca tcattaaaat
     1141 attcattata gtattgtaat gcttcctccc gctctgtagc ggaaatattc tgcaacagac
     1201 tttccagctg cctcataaac tccgttctat tcattctact atgttctccc atctgcgcct
     1261 gtttacgcct ctgacctgtc acttagtttt ccttcaaaca atcccgtgat cttatcgctg
     1321 tagctgcgcc actcactttc atataaatga agctgcgccc agcctctgct ggtgactttg
     1381 taataacgcc tcgttctgcc actgcattcc cggtcataga cttccagaca ttcgtccttc
     1441 tgtaatcgcc gaagcacggg atacagggtg gattccgaca attcgatcac ctgccggacc
     1501 tcctgcgtga tcttgtagcc gtaggttcct tccggttccc gggccaccac cgccagtacg
     1561 atagcatcca gcagagctgc tcctgtatta aaaagcatcc tatcgctcct ctctgaaatt
     1621 tgcggctatc cgcctttcct tacaatatta tatgccgtat agtattgctc tgtggctata
     1681 ctatacggca tataatatca tgcgtcaata tagagaaacc gtaagaaatt ctaaagactt
     1741 tccaaagatt ccacaatggc tttgccatca tgaccgggga tgctctgtta gttttccatc
     1801 agccagaaat atgcactgta cgccggcaga agactaccgc cgccaaagtc atgtttctct
     1861 ccggtgatca gatccaccgc cgtgccgcag ccggcatcaa aatgcgccat gaaatccgct
     1921 ccatccgcat tgatagccac catgactctc tcatgctccg actttctttc gaaaatgcat
     1981 tgcttattgg tcagcagtac tgaacggaaa gcaccgtaat tcaatgcttc cgaatgcttc
     2041 tttgcctctg tcagcttgct gatccagtca cacagaccat tccactccgg cttatcaaag
     2101 tagggacgca gtgcaggatc gccgtcttcc ttgcgggcct tgaagcccca ttcacttccg
     2161 taatagacac agggaatgcc cggcataccg aatgccatgg catagatcag cggcagatga
     2221 ttctcgttgt tcaaaatact tgccacacgg gtcacatcgt ggttgtccac aaaggacagc
     2281 agatgcttgc ccttatacag ggtccagttc tccggtccga actgacgtaa cagggaatgt
     2341 acgatctcga acatattcat actgttgaaa ctgctgtaaa gtcccttgta gcactcgtag
     2401 ttcgttgcgg aatgcagcat ctgatcgttc atcagacggt tgtagtctcc gtgaagcatc
     2461 tctcccaata agaagaaatc ctgtttgtaa gcgctggtta cttcccgcag tcttctgacg
     2521 aaattttcat ccagactgta agccacatcc agtcgcagac cgtcaatacc gaattcatcg
     2581 atccagccct tgacactgtc cagcagatag ttcaccacat cgggattctg caggttcagc
     2641 ttcaccagat caaagcagcc ttcccagccc tcataccaga gtccgtcgtt gtaattggaa
     2701 ttgccaccga agtcgatccg gtagaaccag ttcacatacg gtgaattctc ccggttgcgg
     2761 atcacatcct ggaactgggc aaagcctctg cccacatgat tgaacacacc gtccagcacg
     2821 acccggatcc cggccctgtg caatgcgtcg cagacctcct taaaatcttc gttggttccc
     2881 agtctcacat cgatcttacg ataatctctg gtattgtaac catgagtatc cgattcgaat
     2941 accggggaga aatataccgc gttcactccc agcttctgca tatgggggat ccagtcctcc
     3001 acatgtttga tctcatgtct ctgtactccg tcattttcaa aaggaactcc gcagaatccc
     3061 atgggataga tctgataaaa aacactttca tacgcccaca tattagttac cattcctttc
     3121 tgccttaatt tcataccttg tactcgtaat gcttttccat tgactggttc agtactgctt
     3181 ttatttattt gtccacatca attcttttgc tattatacac catttttgaa ccgtctgtcg
     3241 ttttttctgt gtaaaaccac aaataatcca caaaatccac cgacttatcc acatatacac
     3301 atgtgtcata tgtggttatg tggattgtca tatcggatat caactcatgt atcacattga
     3361 aaatcatccc ttttgccaca accaggcatc ccagaagcgc tccacctttt tggctaccgt
     3421 atccacggtc acaggctgca cctgttccca ctctctgggg aataatctcc ggtaggaata
     3481 ctgctgaaac cgatcatcca gcagaacaat gatacccaca tcctccgccg tccggatcac
     3541 gcggcctgcc gcctgcaata ctttattcat tcccggatag cggtaggaat aatcgaagcc
     3601 gttctctccg tcctcatcaa aatagccctt gaggatctcc cgctcacagc ctacctgggg
     3661 cagtccggtt cctactacaa tgactccgat cagactgtcc ttcttcagat cgatgccctc
     3721 tccgaagatt ccccccagca cgcagaagcc gatcagcgtc tgctcctctt cctcctcgat
     3781 ctccataccg atgagacttt gcaggtcaca ttcctcattg ccccggaagc gattcaggaa
     3841 gtattcacgc tcttcctcat tcatggattc ctgttggacg agacattctt cttcctctgt
     3901 cataaaatac tgttcataga tctcgtaaat atgattcata aaggaatagg acgggaaaaa
     3961 tatcatgtaa ttgccgtgcc ggttcttcac cacttcgtga atgtaacggg caatattgta
     4021 gtattcttcc tgggatcttc tgctgaactt gctagtcaca tccccggcga tcagtatggt
     4081 acgcttctcg ggatcaaaca ccgagtgggc atacacctcg taatcctcct tctcaccgcc
     4141 cagcaggttc ttgtaatact ggatgggcag gaaggtagcg gaaaaaagga tcgtactgcg
     4201 tcctctgagc atgcattcct tcaggttttc tctgggattc acgcagaaca gcttcagttc
     4261 aaagctgcca tcctcacaca atctggtata tttcacatag ttttcatctt gtctctcgta
     4321 aatatccaga aaatgactga gcttgaaata gaagtccagc agattctccc gtacttccag
     4381 gctgaccttc tcctgctcct ccagataatc actgatcaca gcgtgcagtc tggtcaaggg
     4441 ctgcaccagc atgtcaatat catccaccag ccggtacccg tcacagtccc gcttcatgga
     4501 cagcagctgt gcgttacact tctccagcag ctggctgatc cgttccgcat agccttcccg
     4561 cacaaaggta ctctttccgt cagacttttt ccctttgtgt ccccttacat agagggaatc
     4621 ggtaccgtct gtttcctcca gggtaatctc ctgtggcagt tgttttgaca tgcctgtcat
     4681 ttctaatgtc atctgcccgg aaatcccgtc tttatcacgc tttttctggg ctgcttcttc
     4741 catttccgac gtgattgtct gtttgatctc ccgcttgagt tccaacagat cttccttccg
     4801 tagcggtgca ctgtacatct cccggccccg ctctaagagg ttatgtgcct catcgatcag
     4861 aaatacataa ttcccctgac tgccgtcccc gaaaaatctc ttcagataca catggggatc
     4921 aaagaggtaa ttataatctc cgataattcc atctgcaaac agacttaaat ccagtccgaa
     4981 ttcaaaaggg catacccgat atttcagggc atattcttct attttgcttc tggtaaagct
     5041 ctcttcgtgg gtcagaagat catagatagc ctcattgaca cggtcaaaat gcccctttgc
     5101 ataagggcac tgctccggat tgcactccgt atgaccctga aaacagatct tctccttggc
     5161 tgtcaggata atgctcttaa agcgcagtcc cttctgcctg agcaaagaaa aggtgtcctc
     5221 cgccaccgtc cgggtgatgg tcttggcggt gagatagaac agcttgtctc ccatatcctt
     5281 tcccatggcc tttaccgtgg gaaagatcgt ggatatggtc ttaccgactc ccgtaggagc
     5341 ttcaataaat aattttctct tatgataaat ggtctggtac acatagctga ccagctcttt
     5401 ctgtccatcc cgatagtcga agggaaactg caactcagca atggactgct gccgcaattc
     5461 tctccactgc caggtatagt ccgcccattt gcggtaatca ctgatcagct gctggaacca
     5521 ggcttccagt ctgacgaatt cataatcttc ataaaaatag cggatatctt ccgtctctat
     5581 attgcaatag gtcagccgca cccggatata gggcagctcc ttnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nncttccgtc tctatattgc aataggtcag ccgcacccgg
     5761 atatagggca gctccttctg cagactgtac atataggcat agcactttgc ctgggcaatg
     5821 tgcaggggta acggtgcctt cagcttcgcc agatctctgt aagttccctt gatctcatca
     5881 atggtggtct ccccattctg ctcaatgata ccgtccgccc gaccctccac gataatgaca
     5941 taatcctctg tggggtgtgt atatttcaga gccacttccg cctgatactc cgcgcccatg
     6001 cggcgttgga tcatgcggtg gatccggctg ccttcctgca tggcattctc aggggagccc
     6061 tgtctcctgt catcgatgtc accactgcgc agaataaact ccaccagatt acgtaccgat
     6121 attctgactt ctcctgccat gcggcttcac ctcccttgac aaattcctat cataaacgca
     6181 tgcctgcggc ttgttccgtg ctgcacactc tcacatgccc ggtatacgaa aactccccag
     6241 gtaaatagta ctctatctta cctggggagt caaatcacat tctgttatca caagatgttt
     6301 tagcacgcga tcgcatactg tctcaacagc tcttcgaatt tatcattgta gccgtcatag
     6361 gacacggtca acggcttcgt gaaatccaat ccatacactc caaggaatga tttggcatcg
     6421 attacgtatc tgttatagca tatatcaata tcaaaatcac actttgaagc tacatcaaca
     6481 aaatgcttca cgtcttcagg tgtcaaacgg atcgtcttta acatttcttg ttccttcctt
     6541 tcaatagtaa gttcgatcgt aagtaaaata atacaccggg tactttcccc tcacatctga
     6601 aatcattact attatagcca accatcccca aatgtaaagc ggtttttttc ttccagaaaa
     6661 cgcacatttt gggcattttt accactctgt tatcattttc acaatatatg ttcgccgtat
     6721 ggaaaacctt ttcggcgttt tgtatatttg ttgcgttacc gcggtaaaat ataaatccca
     6781 atttggcgaa atctaccgaa attccaccat atattcttgg aacctcagcg gaagcatatt
     6841 tcgttgcaga tccctgtttt cacgttcttt tatggctatt gtatgacaga aggaacggaa
     6901 aagttcccgg tagtgctgtt ccgcagcgga cagtttctcc gtctcagatc ttactttctc
     6961 ccaggcatcc ttcccttgca gcgtatacca gggctttccc gccggatgta ttccgaacag
     7021 ataccggcct gcatcgaaca ccagaaagtc ttccatggga aagcggtctg caaaatgcac
     7081 catcagaaag gtcagaatat tattcttcgg cgcgatcttc gaatagagga ttgtattctc
     7141 cagttcttca aaccgcgtaa atcctttcag atggtgatac tcattcccgg cgcctctggc
     7201 caggctgaag gtctggttta catacgtgtc tgccatggca tccagcagat gtcccggcgg
     7261 acacttctcg gaaagcccct ttgcaatggt acggtatacc gcctgggcct tttccggctt
     7321 cggggatgaa agagccagac acaggctctc ataatcttcc tccccaaatc tgcgtttcaa
     7381 tgtccggatc accttcaccg tcttctccgg atccggtatg acctccaccg actccgagaa
     7441 cagcaggttc tcctcgatgg tggtgaccat agtatcccgg atgatacagt gatcttcata
     7501 agtattataa atagcggtga agattccctc cagagaatcc tcacagtgat aaatcttcat
     7561 atgatccgtt tctcctcctg ccgtaccccg ttcgggaaca tcccatcatc aaataaagtc
     7621 atctgccggt aactcatgcc atcgctgccg aaccggatcc gttccttcgg atnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtcatctg ccggtaactc atgccatcgc
     7801 tgccgaaccg gatccgttcc ttcggatccg tcagattccg cacgatatag tcctcctcca
     7861 gcttcgtggg atacatcatc ctgccaccac aggtaataaa ataaacagcc cgtttcatca
     7921 ccacgcccag cttcttcaga tcctgaaacg taagctttgt gctcctcctg gcactaagga
     7981 tccgtctggc gcttttcacg ccgatccccg gcacccgaag cagatctgca taaggtgccc
     8041 ggttgatctc caccgggaaa cattccagat gccgcaccgc ccagtcctcc ttgggatcca
     8101 gcatcacatt aaaaaacggt cttttttcgt ccaacagttc ctccgcctta aacccataaa
     8161 accgcagcag ccagtctgcc tgatacagac gatgctcccg cagaagcggc ggccccccgg
     8221 gaagcgccgg caggctcttg tcctcattga cctttacaaa agcggaatag aacacacgct
     8281 ttaactcaaa cttctgatac agattctctg ctacatttaa aatctgataa tccgtctccg
     8341 gggttgcccc tatgatcatc tgggtagact gtccgccact cacgaaaaca gggctcttgc
     8401 ggtacaggat cagttcctcc ttattcgcat gaattccatt ctggatctgc cgcatgggcg
     8461 tcagaatatt tttccggtgc ttgttcggtg ccagtgtccg cagcccctcc gccgtgggca
     8521 gctccaggtt cacgctcata cggtctgcca gataccctat ccgctccacc agctcgggat
     8581 ccgctcccgg aatcgctttt acatggacat agccgctaaa atggtattga ttccgtagca
     8641 gccatatggt ccgatacaac aattccatgg taaaagtagg atcctgtatg ataccggagc
     8701 ttaaaaacag tccctcgata taattacgcc ggtaaaactc catagtcagt gtacagatct
     8761 cctcaggggt aaaggtggct ctgggaacat cattggactt tcgattcaga caatatttac
     8821 agtcgaagat acattcattg gtcatcagaa tcttcaacaa agaaatgcat ctgccatccg
     8881 aagaaaagct gtgacagatc ccggccttcg tgcagttccc catgtgcttc ccgtcgccct
     8941 tacgttccac tccactggaa gtacaggcca catcatattt ggctgcatcg gtcaggatct
     9001 ccagcttctg catgatggac aattccccgt tttgtataag atattccaca ttatcaccgc
     9061 cttttaccga aaatttccag aatcagaacg tttgttcttg tgtatattca agttagcaca
     9121 tatgttcgat ttttgcaagt ggttattttt tccgaaaaaa ggcttgcaaa atcgtgcatt
     9181 tcgcagttaa ataatatagg aatattttgt aacacacgcc gcccggcggc agaatgagag
     9241 gtcagtatgt cagatattgt catacctaaa aattataagt ctcagctgaa cctgtacgag
     9301 acacaggttg ccattaagac agtgaaggac tttttccagg gattgttagc agagcgtctg
     9361 cacctgctgc gtgtatctgc tcccttattt gtggatccta catccggact gaacgataat
     9421 ctgaacggtg tggagcgtcc cgtgaatttc catattgcag cacagggaga ccgtgaagca
     9481 gagatcgtac agtctctggc caaatggaag cgttatgcct tgcagaaata cggttttcag
     9541 cccggcgaag gtctctacac cgatatgagt gccatccgtc aggatgagga gaccgacaac
     9601 atccattcca tctatgtgga tcagtgggac tgggaaaaaa tcatcacgaa ggaagagcgg
     9661 aatctggaga ctctgaaaga gaccgtccgc accgtataca aggttcttcg caagaccgag
     9721 aagtatatgt ccatccatta cgactacatc gaagaaatcc ttcctcatga catcttcttt
     9781 gtaactacca gtgagctggc agagatgttc cccgactact ccccgaagga gcgggaatac
     9841 tacattgcca aggcaaaagg cgctgtctgc atcatgcaga tcggtgagac cttagagaat
     9901 ggcaagcctc acgatggccg tgcaccggac tacgatgact gggcgctgaa tgccgatatc
     9961 gtggtatatt atccggttct ggatatcgct ctggagctgt ccagtatggg tatccgtgta
    10021 gacaaaaagg ctctgttaga ccagctacag aaggctggct gccccgagcg tgccgaactt
    10081 ccttaccaca aggcagtcat taacggtgag cttcctctta ccatcggcgg tggtatcggt
    10141 cagtcccgta tctgtatgtt cttcttaaga aaagctcata tcggcgaagt acagtgttcc
    10201 ctgtggtccg atgaagtgat ggagactgca aaagagaacg gcttacagtt gttataagac
    10261 atttccattc agacttcaaa atccccggaa gatgaaaatc tcccggggat ctgttttctg
    10321 tttttattca aaaatcacaa atttctcatt ttcaaaggtt ttctcgtatc catatgcatc
    10381 catgaactca ccgatcaaca aaagcttggc attatccacg atgagactcc gttctctttc
    10441 cgtgggctgt aatgcttcca gggcattctc tccggcttcc agatacacgg catatttctt
    10501 ctccaacagt accaccgggc gataagaagc atccgcatcc atccgctcct gcatcttaag
    10561 catatcctgt tccagttgtc cgctctggta ggaatccaga tccggccagg gattgaacgc
    10621 tgcaggcatc tgtaaataat aagacagcgc cgggatctgt ccgtagatca gtacatctct
    10681 tccggcaagt ccccgttcat tcacatactc tgagatcccc tgcatccatc cggcccgttc
    10741 ctcgctcatc cacacacctt ttaatgtctc attattcgtc acctgcgcat cgatatcctg
    10801 tatgccggta ccttcggcaa atgcaaagtt tctgccgaac agtcctacct ggacaaagaa
    10861 caacagaaag aaaccgccca agagacattt taccggcata gcagatatgg tgatccgctt
    10921 ccaccgaaag ctgccaacat atttgcagaa cctgtagaac tgccagtaca tatagggcaa
    10981 tgccagaaac agattgttca tgctggggta tagcttgttg ttactgccca gggaagtgat
    11041 ccatacgatc aggaagatca gtccgctgat cagcttttct tccctcggcg cggagggcgt
    11101 aaagatccgc cacagggtca ccagcagagt caatgtcaaa aacgtcactc cgggccagat
    11161 aatggcaccg taattcgtat attctctggc acagaagccc tggcggtaca gccagaacac
    11221 catcacagcc gccagtgcga tacttcccgc ccattccagg atccgcagaa cctttgttac
    11281 tgtatctttt ctcacgtagg agccgatgag ttccaatact cccacagcga caatccccac
    11341 tacaaggaac acgcacatcc ggatctccca gtacagattc tgaaaatacc agtcgaacat
    11401 gcccatgatc atggatgcag ccgtatagtc cgttgccacc tccgtcatgg aaaacagtcg
    11461 cagaatgccc ttcacatatt catccatgcc gtagcggatc tggatataac cgaataatac
    11521 cagcagtgcc gtcagatacc ccgccagaca catgccggta tcctgtaata atttcttacg
    11581 gagtttcacc cccgctgcct ttttccgcgc ctttattttt tccgcagtat ctgtcgtctc
    11641 cccagcctga ccgagactct ttccaatctc tttgcgcgct tccagccagc agatgattcc
    11701 gtaagcccac acgcccacga tcatggccgc ctccggcaga ttggagaatc gggacagcac
    11761 attacagccc agcgctactc cggcggcaaa cagccacccc tttttccccc gggcaaggcc
    11821 cagatacaga cagaccaccg ataccagata gaacacgtag gtcacataat tgtaaaaaga
    11881 gcccgttggg caccagcaga agctcaccgc agtaaattct cccaggaata ccagaagtgc
    11941 agggatcttc agtactttcg tgcagaaata atacccaagc accgccagca gactgatact
    12001 cagtccggta taaaagttca tgccgatcaa agtcccggcc ccgggcagca aactgaaaaa
    12061 atgtccgatg gctgtggtca gataggtgga aaacagccac atggaatcca tatgctgcgt
    12121 ccccatatat tcaaagttcg catagccgta accggtatcc cacagatcca gtccccaggc
    12181 aatatgccgc agaggataca ggaccaggat tgccacgaat acaaattccc agagacgtct
    12241 ctgactgtca atacccgctt ttttattttt tattatctct gtcactccat tacctctctt
    12301 ttgaacatag tatctacgtc ccgtattttc tctgtcagac tgcgataggg acggatatgc
    12361 aggaatatct tatgcagtcc gtcgcagata gctttccgca caaaatccac cagaattccc
    12421 agtacaaata ctaccacaac aacaagtacc gtccacaaca gcaacggcag tacaccgtcg
    12481 atttgctcca caccaagcca cttctgccag gcatagcgca cacccagatt ttcatgcagc
    12541 agatacactc ccagcgtata gggcgcgatc ctcaccgcca cactgccgat ctttccctgt
    12601 atgctgctgt ccaaaaacag gcagaacagc cccagtgaag ccagaaacgg gaaaatatgg
    12661 ttatactcat acggaatctt caggatcagc tccagactgc ctgttttcaa ataaaacagg
    12721 tgcaaaagca tggcttctcc aaaggttccg aagataccga tcagatacaa caataatgcc
    12781 cgggattttt tctgtaaaaa cgggattcca aatcggcgca ggtaagccgc cgcgcaaaat
    12841 acacacagat accagagaca atcatagcct ttaccgtctt cttccaaccg gaagggcaga
    12901 acagatttta acaggcagaa ggtgacaaac agcagtacta atgccacctg aaactgctgc
    12961 ttcgtcatcc gccgcagtcc cattcccaca aaggggagca atatatataa gaaaagatac
    13021 gctgtcagga accagtaatg tcccatagtc accgggaaca acagggtcag ataatagtga
    13081 gtgctgactt ccgc
//
LOCUS       DAWEJW010000082        12548 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1794_length_12548_cov_61.015529, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000082 DAWEJW010000000
VERSION     DAWEJW010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12548)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12548)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12548
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1794_length_12548_cov_61.015529"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3103 a         3364 c         2487 g         3594 t
ORIGIN      
        1 ttaatatgga agatcacgcc gctcttgggg tagatccact cgtccttgat cgcaatgtca
       61 tagagtccgg ctctctggat gcaggctccg acattggcag tgctttcctt attgagatag
      121 gaatacatag tgacatccat tccattcttg tatttcagct tgatatgggg ctccttggtc
      181 ggatccacca gagtcacctc gaaaatagag ttccataccc ggggcatctt gaaatagatg
      241 ctgaccagat gctccccgct tgcctcgttg cggtcaaaat ccaacttgaa tttcagaata
      301 aatccgttgc tcttgcgttc tacttcaaac tgcgcttcgt cgtcattatc tccgctgttg
      361 tcaattcgca aaatcggcat gaacagatca ttgatcacat cccggacctt gtccgcggaa
      421 atactgttga tgaagtccac agcttccggt tccaccacca gatcctcatt gaaaatgctc
      481 ttggtaaaat ccgcatcctg tccggtctcc agcaatccac cgtccagact ctttttatca
      541 taggcgaaac cgagtctgat cacatcgccc gtgtagttct cgtctgttcc tcccagacac
      601 tttacgctga acttcaattc ctcatcgatc aggtaatact ttccgtctgt ggctccgggg
      661 aatccggctt ttacatattc ctccgtcaga ttcggctcca gattgatcgt gtaatcgaac
      721 attttccgga tcctggcgcc tcttgccctg tcaaaatatt tctccatatt ggtagaaagc
      781 atatccaccg catcttcatc ctttatcatg gaggtaatgg tggatttcag gaaagtcttt
      841 ttatcttcat cgctgaaaga atccaggatc tccggctctc ccatcagcgt cttggtgatc
      901 tgctgggagg tctctctggc gtaggcatcc tgggtcagct tgttgttcag tatatcgaat
      961 aacagaaatg ccgccagcac accgatgaga ttccccacga tattgtcgat caggtcatcc
     1021 agcgtgtaac cgttcttcag tgccagatag gtatatccca caaataccag caatagtgcg
     1081 atcagcacca gtaccatagt agcggacaag gtgtccatca cacttttttt cttcttttgt
     1141 ttctgattat ccatgaattt cctcccccgt aaatacgccc tcatagattg cgggcttccg
     1201 catgtaatat gcccagaacc ggtcaccgta atcataatgc caccattccg acggcagatt
     1261 agtaaatccc gcatctatca tagcataata aagcagacgg cgattttcac ggacctgctc
     1321 gtcctctgcc ccctgtacat gctccggtgc ctcaaaatac gctgcacagg tcttgtccgt
     1381 aaatgcatcg aatccacatc ccataggaag ctctctgccc tccggatcca gaagcgtcag
     1441 atcaatggct cctccggtgg tatgggcggg aggcaccttt gtatccggta ccgggttcga
     1501 gacaaatttg cagatcgcag ctctcctttt tgcctccggc agctcttcta aatgaaaatc
     1561 ccggatgatg tccacagaat acttttcaaa caattcatgc tgcagtgcaa aggggcgcca
     1621 ggcatcccag atgcggaacc ggtaaccgtc cggcagattc cccgccgcct tcaccagcct
     1681 ctcatacatc tccttacgga cgaagcactt ctcttccgca tggtccatat gtaggatcgg
     1741 atacagcatt ttcacatccc acagatcaca gcttttcaat tctataaacg cggaatccac
     1801 aaacgcactg tctatatagc gctccggatc cccgatcacc ggtatcggcc tcatgggatc
     1861 agcctcctta ttatttatat ttatcataat accccttttc caataaatat ggcaagtgtc
     1921 tggtgcattt catataatca gatgcccggt gttggcctcg ctccggtcaa aatcagtatt
     1981 tgcagccctg tttcgctgac aaattatatt tttcttttat tttttgattt taataagtgc
     2041 cgttggtgat tttggcttac catccctata tatctcccct gatttcataa gcggatttat
     2101 aacttcattt aagaattgac ttctactccg atagttaaga gacatctgta gttcctttat
     2161 aggctttggt ccttccatct taagaatagc cataactttt tccgcatctg taaggttttc
     2221 taaatccagt tctatagcct catctctaaa taatctgtca tataatctca aattcaaaaa
     2281 tccgggatat atagatacta ccggctgtaa ttcatctgga ctggacttgt aagattccat
     2341 catggtttga aaaccagttc cacctctctc catcagattt gccacatcca gacaagctgc
     2401 tatgatggca tttcttctga tagacggtat agaaccggct ggatatgcat tgtaatcttt
     2461 tggcagtaac catgaccctg gtgaaactat ttcaattctg tctgaataga tatcaacatc
     2521 aatctgtgtt cccgaaatag aataatccct atgggcaatg gcatttacca atgcctctct
     2581 tacagcttcc ttaggatacg atctgatttc atcccgtcca cccttttctg tttttaccca
     2641 gcctgttttg gtgtttcgtt caataaagtt taatgcattc tcaaatacgt ttgcaataga
     2701 acctcttaat ctgctgctat ctaaaacaat acctgtcttg tttttccctt tccataaacg
     2761 gcagcatatc attgtgtcat cttcattata gttgtctgaa aacattataa atcctgattt
     2821 tgcataccca tcttttgaaa ctatttcttc gttctgcaat tctttcattg ttggaacaga
     2881 tttatcttgt ctgtattcct tacaaagatt aaggtattct gaccactgat tatcagtata
     2941 tgccacctcg cttgtctcat tatctacacc atattttctc tttgaaagtg aaataatttc
     3001 ttccggtgtt gcaggagtcg catttccatc tccctttata aaaactgttt cattgtaatc
     3061 tccttctcta tatcttatta cagaatctgc aggagcaact ttaacagcta atacaaaatg
     3121 ttccgcatta gaatccacac ttctcatcat atatgaaatc ttaatgtgtg gaaaaatatg
     3181 tctatcatta atccttgaaa ttaatagttt tgttttatca acttcttcaa gatcaattcc
     3241 aaaggcctcc ccatcgtttg atacacctat aaataaggtt cctcctttat catttgcata
     3301 tccaataaga gtttttgccc atttaatggg attatccgga ttcaatactg ctttatattc
     3361 ataacttgta tcttctgtaa taacatctgg atatatctct ttgattttca tgaatctcct
     3421 ccatataata atgttcttgg cataactata tcaaacatta ctaaacatta caacaacatt
     3481 acaattacat cataggtgaa aatttacttg catttctgcc tatgatacag taggataagg
     3541 attttaaata atatatgaca ttatataaac attattacaa cattattaca acattactta
     3601 aacattattc actataatga caggtacaag tcccattcgt aggcatcaaa gtcgggggct
     3661 tttgaccccg acatcattaa atacaaaaac ggcatgcgag tgcctatttc tcatagaaat
     3721 tttaacattt gccatttccc gcaaggcttg ttaaaatgag gatatgaaaa agaaaaattt
     3781 atgtaaaacg ttagtaaaaa ccatgtgtat cgtgctgggg atcctcatcc tcgcactgtt
     3841 ggcttacatc gtctacctct atgccagtta ttacaggatc gaggacaatc aggaactggt
     3901 ggtggaggca ccggtcgacg ataccaccac aggtgcggct gcggtgctgg ctacggatac
     3961 ggaatattcc gcagtgacct acaacatcgg tttcggtgct tatctgccgg attatagctt
     4021 cttcatggac ggcggtacct cctcctgggc ggagagccct gagaccgtac agtatgccat
     4081 aaacggtgcg ggagagctag tgaagtcatt agacccggat tttgcgctga tccaggagat
     4141 cgatctggat gccaccagaa gctatcatac ggatgagtac gagatgctgc gcaacattct
     4201 gccggaatat actactgtat ttgcgcagaa ttatgattcc gccttcctgt tctatccgtt
     4261 tacacagccc cacggcagca gcagggcagg actcggactg ttctcgaaat atccggtgtc
     4321 ttctgctctg cgccgaagct tccccatctc cacttccttc agcaagttct tcgatctgga
     4381 ccgctgctac agcgtctccc ggatcccggt ggacaatggc aaggagctgg tgatcttcaa
     4441 tctgcatatg tccgcttacg gcaacagcga tgccatccgc cagggacaga ttgagatgct
     4501 gtgcaatgat atggcaaaag aatacgaagc cggcaattat gtgctctgcg gcggcgactt
     4561 taaccacgac ctgaaggctt ccgaagagga tacggagagc tgtgagtcct gggcatttcc
     4621 ttttcccaga agtgagcttc cagaacattt taccttctgt ctggatctgt tgaccgaaga
     4681 ggagcaggca gccctttgga acagcgcccg taatgcagat atggaatatg ttccgggtgt
     4741 tacttatact gtgacactgg acggattcat tatctccgac aatatcgagt gcctgtccta
     4801 cgagaatgta aacaccggct attcctactc cgaccatgat cccgtgaagg tggagttcat
     4861 gttaaaatag tgtcaagatt tacaaagtct gttgacagtg cttgtccttt tttgtagaat
     4921 gagggcacag ttaagaggga gtagttcccg gatggtttct gatagaatgg taacctggaa
     4981 tggtaaccga ataatacagg cagctgtccg gaatgataat cgtcatcacg tccgtcggac
     5041 cggttattat tgtaagcaac gagactttta tcgatcattt ttgatgcggt aaaagtctct
     5101 atttttatac ttttttctaa gaatacagac gcttgctgca ggaaaggaca gcttatggaa
     5161 tacatattgt tactcgtagg atttatactt ctgatcaaag gtgcagactt tttcgtggag
     5221 ggaagttcct ccctggcggg gatcctgaag gtccccagcg tcatcatcgg actgaccatc
     5281 gtggccatgg gcaccagcgc gccggaggct tccgtcagca tcaatgccgc actggctggc
     5341 agtaatgata tcgccatcag taatgtcgtc ggctcgaata tcttcaacgg gctggtggtg
     5401 gtaggtatct gcgcattcct gcacagcttt atgccccacg gagagatcct gaagcgggat
     5461 atgccgttaa atatcctggt aaccgtcgta ttatgtctga tgtttttaga tggcagctta
     5521 agccgcattg agggtgcagt cctgctgctt ggcatgatcg tatatctggg atttatgatc
     5581 tattctgccc ggaaaaacag agaagagggc gaacccggca agatcctctc tcttccccgc
     5641 agccttttgt atattgccgg aggtctcgct gccgttatct tcggcggtga tctcgtcgtg
     5701 gataaagcgt gcatcatcgc tacgaatttt ggtgtcagcc agaatttcat cggactgacc
     5761 attatcgcca tcggcacctc cctgccggag ctggtgacct ccatcgttgc cacgaaaaag
     5821 ggagacagcg ggctggcgct gggcaatgcc atcggctcca acctgttcaa catcctgttt
     5881 atcctcggaa tgtccgcggt gatcagccct ctgcatgtcc tcggagagtc cgtcatcgac
     5941 accgtactgc tccttggcag tgccattctg ctcttcgtat tcgcccgtac cggcagacgc
     6001 atgacccgca gtgagggggc tgcgtgcgtg cttctgtatg ttgcatatac ggcttacctc
     6061 ttcgtgaggt aacagtgcgg ctatagacgc acaaaaaagc gcacctaagg ggaggtgcgc
     6121 ttttttgtac aaatatcctc ttccgccttg ggaagaattt gcactttata cattattttc
     6181 cgttatacct gctttccaac tctgcgaaaa aaactttgta atctctacct tttccgctca
     6241 acatatcttg atagctatcc attacttttt ggcgaactac attattatct actttgctat
     6301 ccgaatcgac aatatgtctg ttcccttgag ccattacttc catagcctac atcctcctta
     6361 ccttaaaatc aaatccggtc taatatgttc aacggtattc tcacacattc cgattttatt
     6421 atatccgact cttcacgttt aatcaacata taatcactga attcagatct catgtaatac
     6481 aaaagagcac tcccgcatgg gaatgctctt cattatttgt ggcagatatc tctccactgg
     6541 cagtcgccac agatgacctt gccatatccc ggctgcagaa tcttctcttc cacgatccgc
     6601 tcaaattccg caaaagtcat ttcctgccct gcctttaatc cggtgtattt caatacaccc
     6661 gcatcatacc ggtctacctt ctccgcagac tcgcagctgc cacccttatt atgggggcag
     6721 gcgctgcaga tctcatccgt cgttccgacc agctgcaggg atgccggtcc gtttttcccc
     6781 agaagccatt ccttcacctc cgccatatgc ttagtgaacc cttcactgta tcccttccct
     6841 tcgaaaaaat acaggcacat gccgtggtgc ggacggatcc ggtaagttgt tgttacttcc
     6901 atatccggtg tatcctttat tcctgtccgt tcagcttcag agccttagac agtctcttgc
     6961 tcagagtcag tgccagtgcg atcttcacca gatccggaat gatgaaagga attacacacc
     7021 aacccagtac ggtagccaga ccgacagctc cggaattctg cgcatataca accatgaacc
     7081 acaccgttcc gatagcatag cacacgatca gacccagtac catggagata cccagcaccc
     7141 atgtcttacg tccgaacagc ttctcgatca gccacatcag cagtgcggag aataaaaatc
     7201 cgataatgta accgccggtg ttgcccagta actgaccgat accaccagag aacccggcga
     7261 atacaggaat gcctaccgca cccagtaaaa tatagatcaa taccgcaagg cttcctctct
     7321 taccgcccag tacgccgact gccaggaata ctgcgaaggt ctgtaacgta aaaggaaccg
     7381 tcatcgggat ggagatccag gagcagatcg cgatcagaac cgcaaatact gcaatgtata
     7441 ccatgtcata agtcttgcta cgagttgttg tctgtgttgt cataataaaa aaccatgcct
     7501 ttctgtcaga tgtcttttgt aaaaacctct gttagaatag catggtgttt ttcaattgtc
     7561 aaccttttta taatatggtt aacaatcgag tagattctct ggtcaggact gcttcatcgc
     7621 cttcttattc cggtacatat cctcatctgc acgctcacgc accttctccg gatccgtatc
     7681 atccgccgtg aatacagcat acccataggc aattcccaca ggaacgggaa tgatgttctt
     7741 atgattcaag atctctacat cctgatcgat cttatgcatc acacgttcta tcttaaaata
     7801 tcttcccttc ggaatgatac agcagaattc atctccgcca atgcgataac atttcccata
     7861 gcgttcaaaa ttgtcctcta tgatatgtgc cgcgttgata atatactcat cccctgccct
     7921 gtgaccatat tgatcgttac actgcttaag attattcagg tcaaaggtca ctatgacata
     7981 cccttcaaaa tccttctcat ttttcacaaa ataatcataa gcatttcgat tgtacatgcc
     8041 ggtcatggaa tcattcaggg caaactgttc caactcctcc accctgcgac cttttttcag
     8101 ccgggcaacc gtctgtctgg cggattctat tcccagcacc acaataaaga tcaagaaaga
     8161 gattctgcca aagacaccta catcattgct ggttttataa taaccactca catctacaat
     8221 ggtagcaaaa aatacaagca gcagtgcccc tacacatgcc ttcagcctct gatccagctg
     8281 ccttctggca attttgctac agatgaccac cagcaggtat agaatcatca gaatgatcaa
     8341 cgcatgcgtc atccacaggc ttctccggaa ctcatatatc tctgttgcat tcagtatatg
     8401 cgtcaggacg atcaatgcca gattagcatt acagataatt ctacaaaacc ggtcatccct
     8461 gatctccaga aagctcttca caaacaggat aaaggacatc ggcaacagca tgagcgtagc
     8521 aaatgccaga taagagcatc cctgcctgtt ggccaggagc agcgttgcca catccgtctc
     8581 attggccgac catgttccca gcaggatcga aaaaataccc agatacaata aggttccgtc
     8641 gatccggctt ccgcagcgga taacgatcca ataaatacta atatacaatc ctatcagtat
     8701 caccatcatg ctgatgagaa aggcaggcat tgcccggcgc atcacccaac gatagatatt
     8761 atagccgcca ccgatataaa acgtcttctg ctggcctgcc gtttccggat aacaaggggt
     8821 gaactgtacc tgcaagctgc tcacattact tgaaaacctg acaatattcc aggtccagcc
     8881 cggagtattt ccccatatgc cgggtacagc atccagttga tagatcagct tcccatctgc
     8941 atacaccaca atctcctgat gtgtcgaata aaacacgatc gagttcgtct catcatcgtt
     9001 ttgatccaat ataaaatcgt acagctccct gccgtcatcc aagacgctga ctgcgtccgg
     9061 cgtacactcc tgtaatgact ttacctccgg cacactgtct gaggtgcaga tcatgcacag
     9121 aagcaccata gcaaatacga ctgatacaat tataataagc tttccctgtt ccttctccat
     9181 tacttccctc aatatccaac gtgttgtttg gtactatagt actagtttcc ttatcatttg
     9241 tcaacgcaat gtcgtatcta tctcacgttt gaaaatatta taattctgct catccctggg
     9301 ggaacaataa atggccagtt ctatggtttt aaaacatttt ctgtaacgag cgatcacatt
     9361 ggaaaacgca gctgccacag cctccggatc attctcaaaa gctccgcatc cgaacgcccc
     9421 cagaatgacc acttcattgc ctcccgccac ggccacatcc agaattctgg tcagacgctt
     9481 ctcatgcaat gcctgctgct gtgctctcgt catcttcacg gtcttattac cgtctccggg
     9541 attcatccca ttggccggga gcagacgcag attcggtgct gcacaggtga tcacattaac
     9601 ggagtaccag tctttttccg gaagtggtga cggtgacgca gtatcggatt tgaatacaat
     9661 gacttccggc gtatagatgc agtcatcatt gtggatcgga tcctgcgcat atctgtgtgg
     9721 agaataaaag cctgcccaca tttccttcgt attcagacaa aaatacaggg tggagcagcg
     9781 gcacaggcat tcctcctggg cagagcttcc cttggtcaca ccacctccgg gattcgtcgc
     9841 tgatgcaaaa ttgtgaacac ataccctttc tcccggatac ccggcagctg cctcatagct
     9901 tcgcttcttc gacactatga tttccgcatc cttctcatat ctgtcacgat tctgtgcaag
     9961 ctccggcatc aggtccgtga cttccatcat ctccgggatc agcacctgtt ctgccacaga
    10021 gcgcttcact gcctcttgca gccccggatc ttcctttacc cgtttttctg tatcctgaaa
    10081 aattctaaca ttctcatccc ttgccatgat ccgtttctcc tcccatctgt cccggcactg
    10141 gcactgcagc tgcactttcc ttctgctcca gcggcagtat cgcatagtat accagcgtgt
    10201 gcttgtcatc cggctgacag tcccggtgat aatggccgaa ataccaatgc ctgtaatgca
    10261 gcttctgttc caaagtctcc aggtagtccg tcagcagatc cggggcacag gagcgtcccg
    10321 tcccggcatt gatccggtcc tgcaacgtgc tggcacaaca gtgtgtgatc acataatcta
    10381 cctcgtaatg atatcgttcc agattgcgca ggccctcctg cagttctccc tctgtcggca
    10441 gctcctgggg ccaccagctt tcctgaagga tccgataggg aagataattc cggtctgccc
    10501 tgcgcttctg ttctgcgaaa tccaccgtct gacgatccat aattcctccc tgaatgtcat
    10561 gggaagatgc ccctccgaag gtgaaaaagg tcttgccgtc aatctcgaag acctgtcccc
    10621 gttccagcaa tatcaccctg tcccggataa tgtgccgcgc tttcccgcca tgccattctt
    10681 ccagcggata ttccccgatc atatcatagt tttcatggtt tccttgtacc cacagaatgg
    10741 tgtaaggttt ctccgcaaaa tttttacaat gatactcaaa agttttatcc ttcgcccagc
    10801 acagtccaaa atctccgcag ataatgatgt aatcttcttt tgtcagttcc atgccggttc
    10861 tccgtaatct tttcgcggaa aatcttgtga aatccccgtg ggtatctccc gttatgtaaa
    10921 tcatactgct ctccctgtgc actttctctt ccattccgca ctatgcggca tttccccaga
    10981 tatgactaga agagggcacc aggtcctctt ctaccaaaag ctccatcggg cttacacgat
    11041 acaaaatagc taaagcaatc aaattgtcgg cctgaggaaa gcactttccg gcttcccatt
    11101 tatacacggc ctgcgtggat tcaaagccca tgtattccat gacctgcttc acggagatct
    11161 tccgctcctg acggattgct ctcatccgtg cgccgctggc cacaatgtca aaatgcggtc
    11221 tttccatcat acgcctcctt ccgatgccgt acagcatcca gttgcatatc agtctttccg
    11281 tagactttta ctataacaca aagaatctct tttgcctatt aaccgttggt taaattatca
    11341 caaaatttcc taccagtact gttctcccca gtatctggcc agaatcctct ccatgttggc
    11401 tctccccacc gggttggccg tatggatctc caccggataa aaggtctcac gttctgccag
    11461 ccagtccagc agccggatgg catcgccgcc ctgatccgca taatctccca catcatttcc
    11521 aggttatctt ccgttccggg caacactgcg cgtatcctgt ccggtaacaa tatcacgcgt
    11581 ttcccgcact gagggcatac tgccgtatgg aatgtctctc cggtctctac agaccggtat
    11641 tttttgatcg tcggtccctc ttcgacatcc atccacatga aacgatggct gcaggaagga
    11701 caaatcactt cacgtacctg catttttgtt cctcatccta tgggctctct tgctctctcc
    11761 ttgctgtagg ataatattag catactgcat ggaaagaaat actgtttcag gtaaagttta
    11821 caggagttat tccaatcatt gtgtgtgata aactttcata cctgacttgc caatgttata
    11881 tcaccacacc acaacaatga tctacttcat gctggatgat ctgggctgtc cagccggtgt
    11941 atttgccatg ctgtttcctg aaattgctat ccaggtaatc cacttcaatc tcttcgtatc
    12001 tcgtgcaggg tctcacacct tccagtgaca gacatccttc ctctgtctgg tacggacgca
    12061 gctttttcgt gatcaccgga ttgatcatgg caaactgaaa cgggcctgct gccactgcaa
    12121 taatattttt ccgaacccct atcatatttg ccgccattcc gacacaatga tccagattcg
    12181 ctctcagagt atccagcaga tctgtcacta cctgtgcgtc cacctccgtg gcattctcgg
    12241 atttttgtgc taaaaacatc ggatctctca taattgtttt aatcattgtc ccattccttc
    12301 acgtttcctc agttccagcc cttccatacc tccggctgat aaccgatggt agactgtttc
    12361 ccgtttctga caatcggtgt gcggatcacc gatggatttt ccagcacctt tgccagcttg
    12421 tcctcctccg caatgtaccg gatgagagcc agcgtatcct gatctttccc ctcccagttg
    12481 accatgttct ccaggccgcc gttcgcctgc gcaacggaag taaactctcc cttgctcatg
    12541 cctttttc
//
LOCUS       DAWEJW010000083        12246 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1831_length_12246_cov_59.853416, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000083 DAWEJW010000000
VERSION     DAWEJW010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12246)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1831_length_12246_cov_59.853416"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1981..2080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3396 a         2608 c         3302 g         2840 t
ORIGIN      
        1 ccagctttgc ggacatattc atcatcatgg catccaggac ggtgatggcg gtcatgcatt
       61 ccatgacgac tacagctctg gggacgatga tgggatcgtg gcggccgcgg atggtaagct
      121 ctgtattttc accggagcag ttcacagtct gctgggggcg gaagatggaa ggagtgggct
      181 tcacgtaagc tcggatgagt aaggggctgc catcactcat gccacctaaa gttccgccgg
      241 aatgattgct ggccttgaca actttaccgt tctccatgcg gaagggatcg ttgttctcgc
      301 tgccggtatg gcgggctgct tcggcaccgt caccaagctc cacagcctta acggcaccga
      361 tggacataat ggccttcgcc agattggcat ccagtttctc aaagaccggg tctccgattc
      421 cggcgggaag accttccacg aggcattcca tgcagccacc aacggaatcg cagttttctc
      481 tggcctggtg cagataagca caggcctttt cgtcagcctc tgcatccggc atggcagtaa
      541 tggtggagcg ggcagtgtcc cggttatatt ttgcaggatc cgcatcgatg gggccgatgg
      601 aacgggtata ggcgaatacg gtaataccca tctgttccag aatctttgca gcaatagctc
      661 cggccgccac acgcccgatg gtctcacgac cggaagaacg gccaccaccc cgataatccc
      721 gaaaaccgta tttggcatca aagcagtaat ctgcatggcc gggacggtaa ttctctgcaa
      781 tctcgctgta atctgcggag atctgggagg tattgcggac catcagggag atgggagtac
      841 cggtggtctt tccgtcaaag acaccggaca gaatctccac ctgatcggct tctttacgtt
      901 gtgtagtgat agcactggtg ccgggtttac ggcgatccag atatttctga atctcttctt
      961 cggagagagg aagtccggcg ggacagccgt ccacgacgac acccagtgcc tttccgtggg
     1021 attctcccca ggtagtgatc ttgaaaatgt taccaaatgt tgaaccggcc atgacagttc
     1081 tcctttttcc actgttcaga acctcaccca tgcattcgca aaagcgcacg atgaattgtg
     1141 cgttccggtt gctgcacagt taattttgct catttatgga gcggtgactc tattcgagtc
     1201 accttacgaa tcataaaact gctatttaca tgcaagcatg acatatcagt tttaacgatc
     1261 cttagaggtc agaatagtta ctttttatca taaattatac gcaattactg aaaaaatcgc
     1321 aactttttta tagaaaaagg atgcaacaca aaacaaaata tgctatcata tgacataagt
     1381 actaacggga agtaaaaggt cgacacggga atggaaaaaa ctacggaaga aaaagccaga
     1441 gcgaaacttc tgaaattggg cagaaagaac aaatggaaca gagcggtagc agtgccgttg
     1501 ctgttttttg tgatgtcttt ttttcatatc cggaattatt gccacaacaa cggaaagcgt
     1561 tttgcagtga tgctcggtac ctttttcctg tttgcggttt atagcagctt ttcttttccg
     1621 gcctttatca ccgaggatac cggaacggat tatgaactga aaaataccga cacccagaac
     1681 atctctctgg cagaggaaaa ggaaccggat atggcaggtc tggaactttt ggaggatgag
     1741 gatgtgttgg aacagtcgga ttacaacagc acttcccatg ggtatgcact ggtggataag
     1801 tatgacgctt ccgatattct gcaggcgtcc gaggctattg tgagcgagaa tgcggatgct
     1861 gagaagaccg gagaagacgg cagtgtgaca gaagacgata acgcagagca ggaggaattc
     1921 cgttccgatg actggcgtct ggttctgatc aacaaacaga attccattcc ggatgattat
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctgcgcaaaa tcaaggaaga
     2101 gggcgtaact ttccgctctc atatcgacgg acacaagatt ttcatgggtc cggaggagag
     2161 catgcagatc cagtccaatc tgggatctac cattgccatg gcttttgacg agtgtgcccc
     2221 cagtaaggca gaccggatgt atgtacaaaa ttccgtggaa cgtaccacca gatggctgca
     2281 gagatgtcag gcggagatga agcgcctgaa cagtttagag gatacggtga acccacacca
     2341 gctgttattc ggcatcaatc agggggctat ctatgaagat atccgtatcg agcatgccaa
     2401 gcggatcgct gagatggatc tggacggtta tgcggtcggc ggtctggcag ttggcgagac
     2461 ccatgaggaa atgtaccaca ttctggatga ggtggtacct tatctcccgg tgaacaagcc
     2521 aacctacctt atgggtgtag gtaccccggc taatattctg gagggtgtag aacgtggtgt
     2581 tgatttcttc gactgtgtat atccgacgag aaacggacgc catggacatt tatataccaa
     2641 ccatggaaag attaatctgt ttaatgccaa atacgaactg gatggcagac ccatcgaaga
     2701 gggctgcaac tgtcctgcct gccagagata ctccagagct tatatccgtc atctgttaaa
     2761 ggcgaaagag atgctgggta tgcgtctttg tgtactccac aatctgtatt tttataatac
     2821 catgatgacg gagatccgtg atgccttgga tgcaggccgt tttgcagaat ataaaaaaca
     2881 gaagctggat tccatggcta caccgttaga ataagttaca actgtcggag tttagaggaa
     2941 tttcatacaa ttttatgcaa aaagacttgc cccttaagct atttttgtgt atcatactta
     3001 cttgtacaga aagttttaca ggcgtaacac gccggaatgg aggaacaaat gggtatttta
     3061 ttaactgagt cagaggctgc tgcagcaggt tccggaacca gcatgatcgt tatgcttctt
     3121 gtatatgcag tattgatcgg tgcaatgtgg tttttcttta tgagaccgca gaagaaggaa
     3181 cagaagagag tagctgcaat gctgtccaac ttagagacag gtgactgcgt tctgaccact
     3241 tccggtttct atggcatcgt gatcgacatt accgatgata ccgttatcgt agagttcggt
     3301 aataacaaga actgccgtat tcctatggag aaatctgcca tcagcaaggt tgagaaggct
     3361 ggagagaact aagaagaagt gaaaaaggag caatgccggg ataaggtatt gctccttttt
     3421 gcatatacgg cacaactaat ggcttgatct attttaatgt ccataattgc atttatgtta
     3481 attttcaatg ttgaaattta gtactgtatc agttattatg aaataagtat tttacgtaaa
     3541 aatacaggaa atatcacagg aaacgaggaa aataacacta tggagttaca tattacctgt
     3601 attcccggcg atggcatcgg accggagatc gtcagggaga cgaaaaaagt tttagacaaa
     3661 gttgcgcata aatatggtca taaaatgatc tataaggata ttttaatggg tggagcctct
     3721 attgatgtcc acggtgtacc tctgaccgag gaagcgattg cgtatgccaa agcggcagat
     3781 gcggtgctga tgggatccat tggcggcaac actactacct ctccctggta caagcttcct
     3841 cccgagaagc gtcccgaggc gggactgctg gcactgcgta aggcattgaa tctgtttgct
     3901 aatttaagac ccgcagtact ttacgatgaa ctggcagcag cgtgccctct gaaagaggag
     3961 atcagcaaat ccggatttga catgctgatc atgagagagc tgaccggcgg actttacttc
     4021 ggtgagcgta agactatcga agagaacggt gtgagaaaag ctattgatac cctgacctac
     4081 gatgagaacg agatccgtcg aattgctatc aaaggttttg atattgccag aaagcggaaa
     4141 aagaaagtta ccagcgtgga taaagccaat gtcctggatt cctccagatt gtggagaaag
     4201 gtcgtggaag aggtcgcgaa ggattatcct gacgttactt tggaacatat gctggtagac
     4261 aattgtgcca tgcagctggt gaaggatccc gcacagtttg atgtgatcct gacagagaat
     4321 atgttcggag atatcctgtc cgatgaggca agtatggtga ccggttccat cggaatgtta
     4381 gccagtgcca gcttgaacga tacaaagttc ggtctctacg agcccagcca cggttccgct
     4441 ccggacattg ccggtaaaga tattgcaaac cctatcgcca ctgttttaag cgcggctatg
     4501 atgcttcgtt acagctttga cctggatgaa gaagcagatg ccatcgaggc agcggtgaag
     4561 aaagtattgc aggacggctt ccgcaccggc gatatcatgt ctgagggctg cacgcgggta
     4621 ggctgtaccg agatgggcga ccttctggca gaaagaatct gacaacaatt aagcggttac
     4681 atttgcgaat agagaagaga aagagagaca aagatatgag aagtgattct gtaaagacag
     4741 gaactgccca ggcaccccac agaagtctgt tcaatgccct gggatttacc gaagaagaga
     4801 gaagacgccc catgatcggt atcgtcagct ccttcaatga gatcgtaccc ggacatatga
     4861 atctggataa gatcaccgaa gctgtaaaaa tgggcgtagc catggcaggt ggtatgccgg
     4921 tagtattccc cgccatcgct gtatgcgacg gtattgccat gggacatgtg ggaatgaaat
     4981 attcccttgt gaccagggat ctgattgccg acagcaccga gtgcatggct atggcacacg
     5041 gatttgacgg tctggtgtgt attcccaact gtgacaagaa cgttcccgga cttttgatgg
     5101 cggcagccag agtgaatatt cctacgatct tcgtatccgg cggtcccatg ttagccggac
     5161 atgtacacgg acagaagaga agcttatcct ctatgttcga ggcagtggga agtgtggcag
     5221 ccggtaccat gactatggat gaactggctg aattcgagga aaaggtatgt cctacctgcg
     5281 gttcctgctc cggtatgtac accgcgaact ccatgaactg cctgaccgag gctatcggta
     5341 tgggactgag aggcaacggc accattcctg ccgtatattc cgagcgtatc cgtctggcaa
     5401 aacatgccgg catgaagatc atggaactgg tagagaaaaa tatccgtccc agagacatta
     5461 tgacaccgga agcattccag aatgcactga cggtagatat ggcgctgggc tgctccacga
     5521 atactatgct gcatctgccc gctattgcaa gagaagccgg cgtggaactg aatctggaga
     5581 tcgccaacga gatctccgcc aagactccga acctgtgcca cctggcaccg gcgggcccta
     5641 cttatatgga agatctgaat gaagcaggcg gtgtctatgc ggtaatgaac gaattgtcca
     5701 agaggggact tttgaatctc gattgtctga cagcaaacgg cactaccgta ggcgagaata
     5761 ttaagaattg tgtaaacaag aatccggaag tcatccgtcc tgtggaaaat ccttactccc
     5821 agaccggtgg tatcgctgta ctgaagggga atctggcacc ggacagcggc gtagtcaaga
     5881 gatccgctgt agcacctgag atgctggtac acgaaggccc ggcaagagta ttcgactgtg
     5941 aagaggatgc tattgatgcc attaagtccg gcagaatcgt ggcaggtgac gtagtggtaa
     6001 tccgttacga aggccccaag ggaggccccg gcatgcgtga gatgctgaat cctacttctg
     6061 ccatcgcagg aatgggactg ggttccagcg tggcactgat cacggatgga cgtttttcag
     6121 gagcatccag aggagcatcc atcggacatg tatcccctga ggcagcagtg ggcggcccga
     6181 tcgcactggt ggaagaggga gacatcatcc gtatcaatat cccggagaac aagctggatg
     6241 tactggtatc tgacgaagaa ctggcagcga gaagagcaaa atggcagccc agagaaccca
     6301 agatcaccac aggctatctg gcaagatatc atgaactggt gaccagcggc aaccgtggtg
     6361 cggtactgga agcaccttct cagaagaact aagcaatcat taaataaccg gagggatttt
     6421 gaaatgcagt taacgggatc ggaaattctg gtagaatgtc tgaaggagca gggggtagat
     6481 accgtattcg gttaccccgg cggagcaatc cttaatattt atgacgcact ttataagcac
     6541 agtggggaga tcaaacatat tctgacttcc catgaacagg gggcggcaca tgcggcggac
     6601 ggctatgcga gagctacggg aaaagtaggt gtctgcatgg ctaccagcgg tcccggcgcc
     6661 acgaatttag tcactggtat cgctacggct tacatggatt ctgtaccgat ggtagcgatc
     6721 accgccaacg ttaccttgcc tttactcggt aaggacagct tccaggaggt ggatatcgcc
     6781 ggcgtgacca tgcctattac gaagcatggc tatattgtga aaaatgtgga gatcctggca
     6841 gataccatcc gcaaggcatt ccatattgcc agaagcggca gaccgggccc tgtgctgatc
     6901 gatattacga aggatgtgac tgcggcaacc tgcgagtata cacctgtgac cattcagccg
     6961 gaggaaagag tgacccgcta caccagagaa gaactgatgc tggtagcgga aatgatacat
     7021 aaggcaaaga agccgtatat ctatctgggc ggcggtgcta ttatctccgg agcaagtgca
     7081 gaggtggcag agttcgcgga acttatcgat gcaccggtat gcgataccct gatgggaaaa
     7141 ggcgcttttg acggcagaag cgagcgctat acaggaatga tcggtatgca cggcaccaag
     7201 acttccaatc ttggcgtcag tgagtgtgac ctgttagtag cactgggagc cagattctca
     7261 gaccgtgtga cgggtaaccc gaagaagttc gcagagaacg caagaatcat ccaactggat
     7321 gtggatgctg cggagatcaa caagaatatc cgtgtggatg catctctggt aggagatctg
     7381 aagaagactc tgaccgaatt gaatgcctgc ctgtcgaaac aggaacatcc ggagtggatg
     7441 gcgcatataa tggaactgaa ggaaaaatat cccctgaaat acgatgacga aaatctgtcc
     7501 tgtccttata tcatgcagga gattgaccgt gtaacgaacg gagaagccat catcaccaca
     7561 gacgtgggac agcatcagat gtgggcggca cagtattatc attatacgaa gccccgtacc
     7621 ttcctgtctt ccggaggatt aggtaccatg ggttacggtc tgggagcctg catcggtgcg
     7681 cagatcggac agccggataa ggtctgcatc aacattgcag gcgacggatg tttccgcatg
     7741 aacatgaacg aactggctac tgccagccgt tacaacattc ccatcatcca ggtggtcatc
     7801 aacaaccagg tgctgggcat ggtaagacag tggcagacac tgttctatgg gaaacgctat
     7861 tcccagacag tactgaagga taaggtggat ttctgcaagg tggcagaagg actgggctgt
     7921 accgccatcc gcgtgaccaa aaaggaagaa gtggcaccgg ccatcgagaa ggccattgcc
     7981 ctgaaagcac cggtactgat cgagtgcatg atcccggagg atgataaggt attccccatg
     8041 gttccggcag gagcccccat cagcgaagtg tttgacggag atgatttgaa aaggcagtcc
     8101 tgatgaaaaa gacgacgaaa agattagttt tggcagtact tttatttttg ctgttattac
     8161 tgaccggata ttttattctg gcattttatt acagagaagg cttctctgta aatacctgga
     8221 tcaacggtgt ctactgtacc ggaaaaacgg tggaggaagt gaattcggag cttttgttgc
     8281 gtacagaggc tccgaatatt gtcatcgtgg acagagaggg gacagagtat acaatctctc
     8341 ttgcggaagc agattatcag gcagattaca gtaacgtact ggaacattac cggcaggaac
     8401 agaatccgtt cctgtgggtg gacaatgttc tgtttcactc ccggagggaa ctttctccca
     8461 cagtgaccgt ggatgtggat gggcttcgga gagcttatga ttctctggaa tttgtacggg
     8521 ccgaacaaca taagaacaag gattacagcc ttgcccggga tacgtcgggc gagtacgtct
     8581 tcacggatgg acttgcggga cgcattgact tggagaaggc ttttttggcg ttggagaaca
     8641 cagtagaaaa cgggcaggag acgttaaacc tgtcagatag tggctgttat tatgacatca
     8701 ctccaaatga gaaccagaaa actctgcgga atctctggaa ggagattgaa cgttatcaga
     8761 gctgtgatat tgtctattgc tttggacagg agagacatca ggtgacggca gcggactgtg
     8821 cctcttttct ggtgacggag aatggtctgc cggtgacgga tcagaccggt aactttgtac
     8881 tggatgaaga agccgtgacg gcgtatgtag agcagctggc cgaaaagtat gacggttatg
     8941 gccgtaccag acaattccac agtaccagag gagatgtgat caccatcgag ggcggcacct
     9001 atggaagcaa gctggatcag aaaaaagaga ctgcatacct gatggagcat ctgctggatg
     9061 ccggtgtaca tacggggaca cagcagtccc atgttcccac ttacgagaga gaagctttct
     9121 gctatggcag ggatgacatc ggagatacct atatcgaagt ggatatgaca caacagaaaa
     9181 tgtactatta tgaaaagggg gaactgcggc tggagacgga tgtggtgacc ggcaacatgc
     9241 gcagacgtat gggaacaccg gagggggtga atttcgtcta taataagcag aaggacagag
     9301 tactgcgggg accgggctat gcctcccctg tgaaattctg ggtacctgta aagggcagca
     9361 tcggtatcca cgatgccagc tggagaaaag agttcggcgg ggacatctac cagactgccg
     9421 gttcccatgg ctgtatcaat acaccgaagg acaaaatgga agaattgtac gacatggtac
     9481 agatcggtac ccctgtcgta atgttttatt aataaaacag acattttctg acataatgtg
     9541 tgtgggatgc gaacattttg aaaataattt aacaatgtag ttgactaaag caaatgaaaa
     9601 gcttattcta tacctatgct ttttataaaa aatttagaaa atatcgagga ggtctttgaa
     9661 aatggcaaga gtgtataact tttccgcagg ccctgcagta ctccctgagg aggtattaca
     9721 ggaagcagcc gatgaaatgc tggattatag aggaaccggc atgtccgtaa tggagatgag
     9781 ccatcgatct aaagcctatg atacgatcat taaggaagcg gaagctgatc tcagagagct
     9841 gatgaatatc ccggacaact ataaggtact gtttttacag ggtggtgcga gccagcagtt
     9901 tgccatgatc cccatgaacc tgatgaagaa ccgtgtggca gactacatcg taaccggtca
     9961 gtgggcgaag aaggcatacc aggaggcttc cctgtacggt aaggcaaata agatcgcttc
    10021 ttccgaggat aagactttct cttatattcc cgattgctcc gatctgccca tcagcgagga
    10081 tgcagattac gtgtacatat gcgagaataa tactatttac ggtacgaaat tcaagaccct
    10141 gcccaatacc aagggtaagc ccctggttgc agatgtatcc agctgctttt tatccgagcc
    10201 ggtagatgtg acaaaatacg gtgtgatcta tggcggcgta cagaaaaata tcggacctgc
    10261 aggtgtggtt atcgtgatca tccgtgagga tctgatcacc gaggatgtac ttcccggtac
    10321 accgaccatg cttcgttata agatccatgc agatgcggat tctctgtaca atactcctcc
    10381 cgcatacggc atctacatct gcggcaaggt atttaaatgg ctgaagaaga tgggtggtct
    10441 ggaagcaatg aaggagcgca acgagaaaaa ggcgaagatc ctgtatgact acctggatga
    10501 aagcaagctg ttcaagggta ccgtccgcaa ggaagacaga tccctgatga atgttccgtt
    10561 cattaccggt aatgaggagc tggatgccaa gttcgtaaaa gaagcaaaag aagccggttt
    10621 cgagaacctg aagggtcaca gaaccgtagg cggcatgaga gcaagtatct acaatgcgat
    10681 gcccatcgaa ggtgtggaga agctggtaga gttcatgaag aagtttgaag cggagaacgc
    10741 ttaaggggga gaaaaatgtt tcagatcaat tgcctgaatc ccatttccaa agtgggactg
    10801 gatcgtctga cagcagacta taaagtaacc gataatataa atgacgcaga gggcgttctg
    10861 gtccgcagtg cttccatgca tgagatggag ctgccggaaa atctactggc agtagcgaga
    10921 gccggagccg gtgtcaataa cattccgctg gataaatgtg cggaaaaggg tatcgtagta
    10981 ttcaatacgc ccggtgccaa cgccaatggc gtaaaggaac tggtgatcgc tggtatgctg
    11041 ttagctgccc gtgatgtggt aggcggtatc gaatgggtca aggcatccaa agacaacgct
    11101 gacattgcca aagccgcaga aaaagaaaag aagaaattcg ccggtacgga agtaatgggc
    11161 aaaaagttag gtgtcatcgg tctgggtgct atcggcgtta aggtagccaa tacagcagta
    11221 agccttggca tggaagtcta tggctatgat ccttacgtat ccgtcaatgc tgcatggaat
    11281 ttaagccgtt ctgtcaagca tgtgatgaat gtggaagaaa tttatgcaga ctgtgattat
    11341 attacgatac atgtaccgct tctggattcc acaaagggta tgatcaataa ggaagccatc
    11401 agcaaaatga aagatggcgt ggtcctgtta aactttgcaa gagatctgct ggcaaatgag
    11461 gcagatgtcc tggaggcatt aaagagcggc aaggtagccc gttatgtatc tgattttccg
    11521 aaccctacga cagcaggaca gcccggctgt atcgtgatcc ctcatctggg cgcaagcacc
    11581 gaggaatccg aagataactg tgcgaagatg gctgtggaag agatgatgga ttatctggag
    11641 aacggcaata tccgcaacag cgtaaattat cctgcctgcg atatgggcgt atgcagccag
    11701 gccggaagaa ttgcaatttt ccataaaaat attgccaata tgttgaatca gtttaccgca
    11761 gtgatcggtg agaagaacat caatatctcc gatatgacca ataaatcccg cggtgaagtg
    11821 gcatatacca tgctggatat tgaaaatgcg gcaacacagg atctggtaga atccttacag
    11881 aagatcgacg gtgtcttccg cgtacgtgtg gtaaagtaag acatttgtct gaatagcaga
    11941 aaaatatgca ggcatcggct ataaaaggcc ggtgcctgct ttacgtttcc ggtgtaatgc
    12001 caaaaacagc ggaaatagtg gtaaaatcag tgtataatgt cagaaactta cagacatttt
    12061 ttcgtaaaag cgttgtatga tatgagggaa tctgttatac ttggcatgga ccaaagacgt
    12121 aagccacaag agaaagaagg aacaaaacag atgaaatttg atatgcattg tcatacaaaa
    12181 gaaggatcta tggatggaaa ggttcctatt gacgatttta ttgcggaatt gatccgcaag
    12241 ggtttt
//
LOCUS       DAWEJW010000084        12244 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1832_length_12244_cov_61.529166, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000084 DAWEJW010000000
VERSION     DAWEJW010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12244)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12244
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1832_length_12244_cov_61.529166"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5806..5905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3304 a         2714 c         2448 g         3678 t
ORIGIN      
        1 tttattcatc atcttcagaa tcctcggtag ggaaaatcag attttcttct gcttcgtcct
       61 catcctcaga aacagcttcc tgtgctactt cttccatggc ttcctcaaga ttgtcgaatt
      121 catggtctcc gttggaaagc ttctcacgta ccttggcaat ctgtgctacc tttacgccgt
      181 tatccagatt gatcagcttt acacctgatg taattctgct caaagtggag atatcctgca
      241 cacgcagctg aataataatt ccttcggtag tgatcatcat gatctcgtta tcatcattta
      301 cagccttggc acctaccaca tcacctgtct tttctacgat cttgtagcat tttacaccct
      361 taccgccacg tttctgtacg gtaaattcat ccatattggt acgtttgccc ataccgttct
      421 cggatacgat cagcagatcg ctgccctggg tctgtaactg catgccgatg atctcatccc
      481 cgggagagag attcataccg atgacaccca tggacgctcg gccggtggcc cggacatctg
      541 tctcctggaa gcggatacac ataccctgtt tggttaccag gaatatctcg ctgttgctgt
      601 cggtgatctt cacctcgatc agttcatcct cttctctgag gttgatagcg gtcagtccgt
      661 tctttctgac attggagaac tccatcagag gcgtcttctt cacgataccg gtcttggtca
      721 ccataaacag attcttgtca tcactgtatt cacggatagg aatcattgcg gtgatctttt
      781 caccgggtgc cagctgcaag aggttcacga tagcagtacc tcttgcggta cggcctgcct
      841 ccgggatctc atatgctttt aatctgtata ctcttcccat attggtgaag aagttcagat
      901 aatgatgagt cgtcgtcatc aacagatcct caatgtaatc ctcatcgatg gtctgcattc
      961 ccttgatacc tcggccgccg cggttctgtg ctttgaaatt atctacggtc atacgtttga
     1021 tataaccgag actggtcatg gcaataatag tattttccct cggaatcaga tcctccatgg
     1081 agatatccag ttcgtcataa ccgatcttac tgcgtctgtc atcgccgtat ttctcagcga
     1141 tagcactcat ttctgtctta ataacaccta ataacagctt ttcatctgcc aaaatagcct
     1201 tcaactccgc aatttttgct accagatcct gatgctcgtt ttccagtttc tctctttcca
     1261 gacctgtcag agcacgcaga cgcatatcca caatagcctg tgcctgtaca tcagaaagtt
     1321 caaatctctc catcaaacgt tcttttgcaa tctgggcatt ctgactgcca cggataatag
     1381 taataacttc gtcaatgaag tccagagcct ttaacaatcc ctgtaaaata tgatctcttt
     1441 cctcagcctt gttcagatca tatttggttc ttctggtaac cacatcctcc tgatgtttca
     1501 ggtaataagt cagcatatcc agcagattca tgaccttagg ctgattgttg atcagcgcca
     1561 gcatgatcac accaaaagta tcctgaagct gtgtatgctt gtacagctga ttcagaataa
     1621 cattggcatt ggcatcctta cgaagctcaa taaccacgcg gacaccttca cggttggatt
     1681 catcacggat atctgtgata ccgtcgatct ttttcagttt taccagctca gcaatcttga
     1741 tgatcagatt ggccttattg accatatatg gcagttcggt ggcaatgatc tggctcttac
     1801 cgttaggtag tgtctcgata ttggtcactg cacggactct gatcttacca cggccggtac
     1861 ggtaagcttc ctcacagcct ctggtaccca gaatcagtcc tccggtagga aaatcgggag
     1921 ctttcacaat gggaagaatc tcatcgatcg tagtatctct gtcttcttct acacgattat
     1981 caataatttt aacaacagcc tgtaccactt cacgaaggtt gtgaggtgga atattggtag
     2041 ccatacctac ggcaataccg gtagtaccgt tgactagcag gtttggataa cggctgggca
     2101 gtacgacagg ttctttttca gtatcatcaa agttcggtac aaaatctacg gtattcttac
     2161 cgatatctgc cagaagctcc atggaaatct tggaaagtct tgcctcggta taacgcattg
     2221 ctgcagcacc gtcaccatcc atggaaccga agttaccgtg gccatctacc agaggataac
     2281 gggtagacca gtcctgtgcc atattaacca atgcaccgta gatggagctg tcaccatgag
     2341 gatgatattt acccatggta tcaccgacaa tgcgggcact cttacgatgc ggcttgtcgg
     2401 gaccattgtt cagttcgatc atggaataga gtacacgtct ctgtaccggc ttcagaccat
     2461 ccctcacatc cggaagcgca cgggatgcga taacactcat ggcatagtcg atatagaaag
     2521 tttccatttt ttctttcaga tcgacttcat ggattttatc aatctcagcc tccgaaggct
     2581 tatcaaaaat atcgtcgttc atgtttcctc acaatctgtc gtaaaatacg actctctatt
     2641 attaaatacc gtaactattc agagcctgat aacggctctg aatagttatt agatgtccag
     2701 attcttaaca aactgagcat tttcttcgat gaattctctt ctgggttcta ccttgtcacc
     2761 catcagtgtg gtaaaggtca gatccagttc ggattcagtc tcttcatcgt agtttactct
     2821 gagcagaata cgtctctccg gatccatggt ggtctcccag agctgttccg catccatctc
     2881 acccagacct ttgtaacgct ggatcttgtt gttctggtca cgacctactt cagagagaat
     2941 gctgtccagt tcttcatcac tgtaggcata ccataccttc ttgttcttct ccagcttata
     3001 cagaggcggc ttcgccagga atacatgtcc ctgcttgatc agctccggca taaaccgata
     3061 gatgaaagtc agcagaagag tactgatatg cgcaccgtct acatcggcat cggtcatcag
     3121 aatgatcttg ttgtaacgaa gcttggaaat atcaaaatcc tcatggattc cggtaccaaa
     3181 agcagtgatc attgccttga tctcagcatt ggcataaatc ttatccagtc ttgccttttc
     3241 tacattcagt atcttaccac gcaggggcag gatagcctgt gttgcacggg aacgtgctgt
     3301 cttcgcactt cctcctgcgg agtctccctc tacgatgtaa atctcgcagt tcttcggatc
     3361 cttatcggaa cagtctgcca gcttaccggg cagagccatg gtatccagag cggtctttct
     3421 cctggtgagg tctcttgcct ttctggctgc ctctctggca cgctgtgcca gaatcgactt
     3481 ctcacagatg gactttgcaa tctgcggatt ctgctctaag aaataagtca gctgctcact
     3541 gacaatattg tcaacagcgc ctcttgcctc actgttaccc agtttctgct tggtctgtcc
     3601 ttcgaactga ggatcctcga tcttgacact gacaatggca gtcaggccct cacggatatc
     3661 ttcaccggac agattcggct cactgtcttt taacagcttt gcgcttcttg cgtaatcgtt
     3721 aaatgtcttc gtaatggcat tacggaagcc ctgtaaatga gtacctccct cgggagtatt
     3781 gatgttattt acaaagctga atacgctctc gttataagaa tcattgtgct ggaaggatac
     3841 ttccacgtac acattattct tctcaccctc aaaatacaga atgttttcgt ataaaggctc
     3901 cttacttttg ttcaggtaag caacaaattc cttgatacca ccttcatagt agaactcaac
     3961 gactttctgc ttcttaccat tttccagagt aatggtatgt atcttaccga tcttgccgcc
     4021 ggtatgggct ttggtggatt cctcggacgt agcaaaagca tctgcaaatg cttcagagtc
     4081 atttgctgta tccgcagaag ctgaagtttc tttggaagtc attgcttctg tggatgctcc
     4141 tgcttccatg gcatcttcct gtgccctctg taacagttca ctgatctgct tcttttcgtg
     4201 ctctgtagac atagcttcca cagctgcact gtcaacgaca gaaccacctt ccagaaaagt
     4261 ttcctcttca gtacgattat ctcttaaaat gatgcgcagc cccttggtca ggaatgccat
     4321 ctctctgagt cttctcttca atacatcaaa atcatatacc gttgtctcag taaaaatcgt
     4381 atcatctggc aggaaagtaa ctttcgttcc ggtcttttcc atagggcagg tgccgatttc
     4441 tttcagagca tagcatacat gacctctctc ataacgctgc ttatatacct tgccaccctg
     4501 gcaaatttct acttccagcc agttggagag ggcatttaca acagaggcac ctacaccgtg
     4561 cagaccaccg gataccttgt atcccccacc gccaaactta ccgccggcat gcagaatcgt
     4621 aaatacgact tctactgcag ggatacctgc tttgtgattg atgcctaccg gaattcctcg
     4681 tccgttatca ataacggtga tggaattgtc ttcattgatg gttacatcaa tggtatcaca
     4741 gaatcctgcc agtgcttcat ccaccgcatt gtctacgatc tcatatacca gatgatgcag
     4801 tcctctgctg gatgtggtac caatatacat tcccggacgc tttcttacag cttccagacc
     4861 ttccaatatc tgaatctgat ccgcgccata ttcatgttct accgcggtat tgttcatatt
     4921 ttcttcgctc atgattcctc acattctgcc ctacaggctg ccaaaattat actcatctgc
     4981 cggagtttct tccggttccg atttcagtcc catattttca tttacacagg tgacagttcc
     5041 ggcggatact ttgaataccc ggttgatttc aaaccggtta tttacaaatt cctccagacc
     5101 ggtacaggta atgatggtct gaatatcccc gatactgttc aacagatagt tttgccggtt
     5161 actgtccagt tcagacaata catcatcaag taataatacc ggcgtatctt tcgtaatcct
     5221 ttttaccagc tcgatctcag ataatttcaa ggaaagagca gcagtacgct gttgcccctg
     5281 ggatccgaac ttacgaatat ccacgtcccc taccagaaaa gaaaaatcat ctctgtgggg
     5341 tcccacagat gtcatttttg cccgcatatc ccggtcctga ctgtttctca gcttactttc
     5401 aaattcatcc agttccacat ccggctcata ctggatacgg atttcttccc gcccaccgga
     5461 caatttttta tgaatctcat aaatgatctc attgagctgt tccacgaaca gctttcttct
     5521 ctctatgatc ttacttccgt aagacaccag ctgcatatcc cagatagtca gagtttcctt
     5581 cagatccgga ttcatataca tatctttgag aagcttgtta cgctgattga caattttgtt
     5641 gtaatgattg agattgtaga gatagaaatt gtccagctgg cacagttcca tatctacaaa
     5701 tcttctccgc tcagagggac catttttaat gattcccaag tcttccggag agaaaaatac
     5761 aacattacaa agccccagaa gatccgctgc tttcttgatc ttctgnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnngtccc ccggtcaaac tgcaggacaa gagaatcata
     5941 attcctgtaa tctgccaatt cgattgattt gatgatcata gtaacctgct tattctatca
     6001 cgatggtctc gccatcatat tccactttat ctccggatac cagttttttg ccacgctggg
     6061 tttctgtctg tccgttcacc tttaccaggc catcctgtac ggcgaacttg gcatccacgc
     6121 cggattctac cacaccggct gcctttaatg cctgaccaag cttaatataa tcatccttta
     6181 aacggatcgt atgcattgat ttaacctctg ttcttaagtt taagcaactg ttgtaaagtt
     6241 cacgggaagg atcaggtaaa tatagctttc ctctgcattc ttgataaaac agggtgcctt
     6301 gggatttacc atatacagat ccacttcctc atcatcgatg acacggagag catcgatcag
     6361 gaacttgggg ttgaatccaa tcatcagatc cttaccctgt ttctgaatat cgatttcttc
     6421 gttcatgctt cccagagcgg aattgatttt aagttccatt ccactgtctg tgatatttac
     6481 tataataggt ttcttatcgc cttcctttac cagaagagta gcacgatcga tacagcttaa
     6541 gaactctttc ttgttgactc ttactttggt ctcataatcg ctggaaagca tctggtcgat
     6601 cttaaaatat tctccctcga tcagacggga tactacaacg gtcttatcaa attcaaatac
     6661 aatatgtttt tccgtaaagg aaatcgttac atcactgtct gcatctccgg gtaaaatctt
     6721 acttacctcg ttcagagtct tgccgggaac aactaccttt ttcggagaat agctgttctt
     6781 cagctggatc ttacgaatgg agatacggtg tccgtccagg gacacgatct tgaactcttc
     6841 tccgttaata tcaaaaagtt cacctgtcat cagcttattg ttatcattgt cagagataga
     6901 gaagatggtc tgtcttacaa cctctttcag tgtaaactga ctcatgacaa tactgtcatc
     6961 tttttctact gccggtaaat aagagaaatc ttcaccagat ttaccgatga tcgtaaattt
     7021 tgctttttca caggtaataa ctaccttgaa ggaagcatca gtctcaatgg taatgtcact
     7081 gtcaggaagt ttacgtacga tttccaggaa catttttgca tccagagcaa tgataccctt
     7141 ttctacgatc tgtccttcta tggtagtctc gataccaagt tccatatcgt tggcagttaa
     7201 cttgatctct ccattggtag catctactaa aatacattcc agaatagaca tggtagtctt
     7261 actgggaaca gcttttgata cagtttgtac gccatttaaa agattggctt tcgaacagac
     7321 taatttcatg tgtgtataac ttcctttctg gttgatttgc gtacgcgcgg atacgcgttg
     7381 tataaaaata ttaaaagaat ttcgtagtca ccttaataag gggtgttgat atgtgcataa
     7441 ccagtcttat tatactattt ttcaaggcaa aatggaatgg aaatcttagt ggatttggca
     7501 gaataaccta agaataactc tcagattatt cacaccgtcc aaattctgtt ccggaacctt
     7561 aaaacaagtt atcaacaggt gaacaccgtt tattccaaag atatacacaa cttgtcctca
     7621 gaataagaaa aattattaac agatatcttc taagaaggat ttatcttctt tgtaataata
     7681 tcgattttat tacgaagttc ttcgttcgtc tggatctccg cagacacctt atttacgccg
     7741 tgaatgatcg ttgtatggtc ttttttgccc aaaagctttc ctatattagt gaaggaggta
     7801 tccgtcagct ctctgcacag atacattacg acctgacggg gctgtacgaa ctcggaattt
     7861 ctcttcttgg aagtaatatc ttccggcttt acgccaaaat gctctgccac tacattaata
     7921 atgagagtag gagtgatctc ctttggcttg ttggggtaga tgacatcctt cagggcttct
     7981 tcggccagag agagtgtcag atctacctta tttaattttg caaaagcaat gatcttgtta
     8041 aacgcgcctt ccagttcacg aatattggat ttaatatttg tggcaatgta tttgatgatc
     8101 tcttcatcaa tctgacgatc gtaggtctct gcatttttcc gtaaaattgc cattcgggtc
     8161 tcataatcgg gcggctggat atcagcaatc aggccccatt cgaaacggga acggaatctt
     8221 tcatccagag tttccatttc cttgggcggt ttatcggaag acagaatgat ctgtttatgg
     8281 gcagcatgca aggcattgaa ggtatggaag aattcctcct gggtactttc cttacctata
     8341 ataaattgta cgtcatcgac catgagaaca tccacgttac ggtatttctc acgcagcttg
     8401 gtcatagaag ccgcattacc actacgaatg gattcgatta cttcgtttgt gaattcctca
     8461 ctggtaacgt agagaacctt tttgtccgga tcctgttcta aaataaaatg tccgatggaa
     8521 tgcatcaggt gagtcttgcc cagaccggga cctccatata aatagagagg attgtagact
     8581 tctcccggag attccgctac cgcaagggat gcggaatgag caaatttgtt attacttccc
     8641 acgacaaagg tatcaaattt gtacttaggg ttcagatggg catcttcata attaatacca
     8701 tagatctgat tgggaacagc catttcattc tcagcaggtt cttctgcctt gacatccttc
     8761 tccaggataa agttgatatc ataaggatga tcgaacattt cggtaatagt tacctgaaaa
     8821 aagctcttat attttgatga aatgtaattc agggcatggg cctggtccga gggaatgata
     8881 atgctcacta catcattcac tacattatga aactccaaag gctccaccca tgtatgataa
     8941 gaaatgtcag agagactgta ttctcttctg acagcttctt tgacagttgt ccagttttct
     9001 ttgattgcgt ccattgattt cctcccgcta agagtcccac tgtacgaaac tccatttcat
     9061 tgtataatag aattaaaaat aattcaaatg taagttatga acaggtcatg gataaagtta
     9121 tccacatatt gttgataaag ttgttcataa ctgggaatat taacagggga tgtggatagt
     9181 gatgtgtata tgtggataaa tacgacaaat ttcgagatat ccacaattac ggtaaacttg
     9241 tccactttta caccgaattt acacagaata tccatgtgaa aaaaataaat atccacaaat
     9301 aatcaacagg ctgtggataa ctcgtttgtg ggtaaatatg gtgtatatct atgtggataa
     9361 ctcgatttaa aaaattttat gaatttcaga aagccctatt tctcttgatt tttcagcatt
     9421 tttattgaat tttgcttgac attgacgggc ttttacatta caatcaatat gctattttcc
     9481 gaaaggaagg cgcatttttt atgcgtccaa atgttgaaaa atatgtttac ctttttatat
     9541 aagaaggttt agagaaggag gtgcctttcc atgaagatga catttcagcc taaaaagcgt
     9601 tcccattcca aggttcacgg tttcagagcc agaatgagta ctcccggtgg aagaaaagtt
     9661 ttagcagcaa gacgtgcaaa aggaagaaag aaattatccg cataggtcac agcaatgtga
     9721 cctttttttc tcttaacttt cacggttgga ggagtgcatg cagttttcgg aatcattaaa
     9781 aaataatcag cagttccgtt ttgtgtacaa gaatggcaga tcttatgcca acaagtactt
     9841 tatcatgtat gtgaaggaaa atggccttga taagaacaga atcggaatca gtgtaagtaa
     9901 aaaagtcggt aatagtgtcg taagacatag aattacccga ctggtcagag aaagttacag
     9961 attacatgaa agtgtattca atagtggttt ggatatggta atcattgcaa gaccggccgc
    10021 agcttctata ggatatgaag aagcggaaag tgcgctgctg catcttgcaa aacttcatca
    10081 tctgataaaa atctattttt atcaggatta aataaaattt gtatatgaaa aaattagtta
    10141 tagccatgat taggttctat cagaaatata tctcaccgta caaaggtacg aagtgtcctt
    10201 atgttcccag ttgttctcaa tatggtctgg aagccattga aaaatatggt gtgatcaagg
    10261 gaggacttct ggctgcatgg agaattcttc ggtgcaatcc tttctctaaa ggaggatatg
    10321 atcctgtacc ttgagaatat ccgatagaag gtggcaaata ttaggaggat aaatgaacgg
    10381 tattcttttg acacagaatt ccacattcat tatcggacag gtagcctggc tgctgggtaa
    10441 gataatggaa ggaatcttcg aagttctgaa cttgatcggt attcctaata tcggtcttgc
    10501 aatcattctt tttacaattg ttgtaaacct gttgatgatg cctcttacca tcaagcagca
    10561 gaaattctcc aaactttctg ctaagatgaa tcctgagatt caggcagttc aggcaaaata
    10621 caagaatcgt aaggatcagg atgcacagtt ggcacaaaat caggaaattc aggcagttta
    10681 tgccaagtat ggagtatctc ctacaggcag ctgtctgtat atgctgatcc agatgcctat
    10741 tctgtttgct ctgtatcgtg taatttatgc aattcccgct tatgttggaa gagtaaaaga
    10801 agctttcttc cctttggtag acaatattat cgatactgca ggtgcaacag agctggttca
    10861 gaatctgacg aattccgcta tgtacagcaa gcagtttacc aatgcaggtt ttgtagcagg
    10921 aacacatact gaatatgttc agaatacgat cattgactgt ctgaataaag catctaccgc
    10981 tgattttgca agtatcagtg agaagttccc ttctcttgca gcagatgtga ccaatacggt
    11041 aagcaagctg gaagagtata ataatttttt aggactgaat atcggcaact ctccttctta
    11101 tgtgttgaag gaagcatggg ctaacggagc atggctttta gtaatcggag caatcgctat
    11161 tccggttttg tctgcattga cacagtggat caacgtaaag ctgatgccac agcaggatac
    11221 ttcttctaat ggtaatgatc aggctgcagc aatggcttct tccatgaaga ccatgaacat
    11281 gatcatgcct ctgatgtctg catggttctg cttcacatta ccatccggtc tgggtcttta
    11341 ctgggtagca ggtagtgttg tcagaagtat ccagcagatc gttattaaca aacacattga
    11401 taagatggat tttgatgata ttatcaggaa aaattcagca aagagtgcaa aaaagctgga
    11461 gaagatgaaa gaacagcagg ataagctgaa tgcttacgct tccatgaata ccaagaacat
    11521 tcagaagaaa gcaaatattt cttccaaggt cgataacagt gaagtatctg ctgacagcta
    11581 ttccgaagta aaagaagcta agcccggaag tatgatggcc aaggcaaata tggtcagaga
    11641 ttataacgag aagaacagtc gtaaataaat tttccttatg attaattgag gaggtaaata
    11701 atatggacat gatcgaagta tctgcaaaga cagtcagtga tgccattacg gaagcttgtc
    11761 agaagctggg tgttaccagt gacaagttgg attatcaggt agtggaagaa ggttcttccg
    11821 gattccttgg aatcggggca aagcccgctg taatcaaggc tgcagtaaag aaaagctctg
    11881 tagaagaagt tgccagagta tttttaaatg atgtatttca ggcaatgaac atggaagttg
    11941 ccattgatat taagtacaac gaagaagaga agagcatgga catcgagtta agcggtaacg
    12001 agatgggtgt tctgatcggt aagagaggac agacactgga ttccttacag tatctggtat
    12061 ctctggtagt caataaagaa tctgaagagt acattcatgt aaaggttgat acagagaact
    12121 atcgtcagcg cagaaaagaa actcttgaga atcttgccaa gaacattgca tataaggtta
    12181 agagaaccaa gagaagcata tctctggagc ctatgaatcc ttatgagaga agaattattc
    12241 attc
//
LOCUS       DAWEJW010000085        11803 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1913_length_11803_cov_48.333844, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000085 DAWEJW010000000
VERSION     DAWEJW010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11803)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11803
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1913_length_11803_cov_48.333844"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2776 a         3038 c         2325 g         3664 t
ORIGIN      
        1 ttcgtgtact ccgacaattt attgcccgtc agacggaaac cgaagtaatc cggcagcatg
       61 agaagtgtct ttgcctgttt cataatctcc ggggtcttct tttccactgc cataagctga
      121 taaatcgtat taaaaatctg cttctgaata ccggttctgg catacagttc cgtctccggc
      181 agtattttcg ccacttccgc atccattccg tcagtacggt gatctcggta gccgtaagta
      241 tctcccagaa tctgatcttt ttcatccagc aatacataat ccacagccca ggtgtcgatt
      301 gccatgctga ccggaagctt gcccagttct ttgcattttt tcattccgtt cagaatctcg
      361 ccgaacagac ggttcacatc ccacagaagc tttccgtcct tatgatccat gccattctca
      421 aaacgataga tctcttccag ctctattttg ccgttctcca tgtgtcccaa aatatgtcgt
      481 ccactggaag caccgatatc cacggccaga taatattttt ccataagata tccacagcct
      541 cccttatctg aaactgaata cctgtgtaag aagcggctgt gctcctgtaa caggatcctt
      601 ctccatgatc agaatatctt tcatatattc atcccatttt ttcactacat cgctctctgc
      661 ctgcactttt gccgcatagt cgacgccctt ttcacattcg taataaccga acagttcctc
      721 gccctgcatc cagatagagt aatttacaat tccagcgttt gccagaactt ccttcatctc
      781 cggccagatg ttgtcatgtc tcttcttata ttcctcctgc attcccggta caatttttcc
      841 gcgccatgcg tatttttcca tacaaaccac catacctttc tcactcatta ttttcgttgc
      901 aaatctgctc tgttacccaa tctctgtcgc aatattcctt tgcttcgggc atcagctttt
      961 tcataatctg tgcttgcaga agcacaaact tgtgctttta acgatattat agaaaacata
     1021 atctgcttct gaaatgacat tatttgctcc ctggactgtc cgattttgcc cgatccgtac
     1081 gataactgtt tattacagca catgaattgt gttatgatac aataagttca cccgtataaa
     1141 gcagccgccg tggataaatt gtaccgcaaa tttgatcacg atagccgccc aagcaaagag
     1201 agtatgaaaa ataccatcgt tatgtatcat aaataataat ttagaaagca caatttttat
     1261 ggaacgtaca ttaacctata caacagatca tttcatatcc cccctgccgg tcagccattt
     1321 cctgaagcag aaagggtttt cttcccagaa tctggtgcag ttaaaaaaag accccaccgc
     1381 cgtgcaggca aacgggattc cctgcttcat gaatcatata ttgcagccgg gagatacctt
     1441 gacgctgcat atccacgagg aacactcctc ggaaaaaatc ccaccggtgg agctgccgct
     1501 ggatatcatc tacgaggacg aggatctcat ggtgatcaat aagccggcag gcatgccgat
     1561 ccacccttcc atgaacaatt attacaattc cctggcaaac agtcttgcct attattttgc
     1621 acagcagaac tgtccttttg tgttccgctg tattaatcgt ctggaccggg atacctccgg
     1681 actgaccatc gtagccaagc attatgtcag cgccggaatg ctgtccacca tgatcgccaa
     1741 caaggcaagt tccggtatca cacgggaata ccttgccatt gtaaaaggct ctgtgctgcc
     1801 ttctgggggt acgattaccg cacctttagg gcgaaaagaa ggttctatca ttgagcgttg
     1861 tgtggatttt gaaaaagggg aatccgctgt tacccattac cgggttttgg acgaaaaaaa
     1921 cgggcacagc ctggtctctc tcattttaga gaccggccgc acccatcaga tacggattca
     1981 tatgaaatac ctgggttatc ccctgatcgg agattatctg tacaatcccg atatggcatt
     2041 gatacagcgt caggcactgc atgcctggaa gctgtccttt gtccatccca tcaccgggga
     2101 gaaaatgcag ttcacagcac ctctgccgga ggatatggcg aaggtagcgg gagatacccc
     2161 atggagcata tcctgaatct gcttaatcta caagcagtca tgcagattcc tatggtgcag
     2221 tattatgcac gcagtttctt cagtgctttc tcccggatca ggcattcacc gtgttcttcc
     2281 agatatctta tcttctgctc gtctgcggca tacaacgctg cgaattccat agcctggatg
     2341 caggctctgc tgtagcgctc gtaggacgca ccgcccttct gcacatagac atagcaagtg
     2401 ccgtcttcca tccggtacaa cgtgctgttg atccgcaggt tcgccggtaa tacggcggca
     2461 tattcgatca ctctgccgat attgcggaag gcaaaaattg caaactgggg ctttgccgct
     2521 tcctctgctt ttccctttgc tttcttattt ttcccggcat tgttctccgt ttcttccttc
     2581 gcctgttttc ccagcgtagc gccgatctct gcctttttct cctcgaagac acgcttcatc
     2641 tcctgcaacg cctgtaacag ctgtgcaccg tttttattct ccagcggttc cttctcggaa
     2701 aataccagca tcaatccttc atccggtacc atcatgatct gtaggtcaaa agcatacttc
     2761 ggcggcttat agcccacctc ttcctcggcc tgctcgataa tctcctgcac aacctctttg
     2821 gctttttcac tgcggaccac aaaatcttta taatcaatct cgtactcttc caattcttca
     2881 ttggacagga agcactttac tgtcttgtca tcaattcgtt ctattttcat agctatcacc
     2941 tcttactgtt atatgcatga tagcataaaa acgtttttat ttctactgtc caaatctaaa
     3001 cattttatat acttcttatt tgcagatact tctttctgtc ttttcttgtt ttactccgca
     3061 aagtaataca actttctgta tcctactgaa cccaatctta tcaatgatgt ctcgtgattt
     3121 taaaatcctg cataacctac cgtatacaaa aaaccgacga aatcttaatg acagccatgt
     3181 caaataagat ttcgccggtt tttaatgtta gatcatacag aatatatata ctcttttact
     3241 ttttcttacg cagatagaaa tatcctgcag cacatcctgt caatactatt acaatcacaa
     3301 taggaatcca aatttttcct gctttgctgt gattacggtt ctggctctct gtgttgtcat
     3361 tttctgcgga aactgcttcc gacgaagcat ccttggacag attctctaca tccataggct
     3421 cgtcagccat aaccagcaga tattcgctgg catgctcata gttgagttca acccatccgc
     3481 tgtctgccac ctgaccgaac gcaactaact ccattgctgc attgtcaggg ttatagtaga
     3541 agagatttgc atatttaccg atattctctg taccaagacg gactgacagt tttgcggtca
     3601 tgccgaagct tccgtcgtgt accagtgaca aaggaatata atcatgtccc tgtgtcgtct
     3661 gtgttatcag ttcagcagga atccgatccg ctgacctaag gattccgaaa tcaatatctc
     3721 ctacattttc tgtgatctcc cgtccttcaa tgatccatgc cagttcctcc tcgatttcga
     3781 ttctcagaga caggttgctt cccattacgc tttgcaatac ttctgcagga acaacggtgt
     3841 cgctgcctgc cacaatatag atctctttat tgctgctatc acttacattc tgacgaatat
     3901 tttcaaggat atccttccat ccgattgcct cagcctcacc ggctgcattg attatggttg
     3961 ctcctgtttt ttctgtcttc tgtgttgttg ccggaacggt cggtgtcgca tttaccatat
     4021 cacttggtgt tgcacccact gtaggacgaa caggtcttgc tgttgctacc ggaaccggag
     4081 ttgcggttgc tgccacagga gaattcgagg tgttgttgtc cttgtcatcc tcatgattgg
     4141 aatcgtttga gggcgtcgcc gtaggtgctg ccgtaggctt tggacttgcc gtaggctttg
     4201 gacttgccgt aggctccgga gtcggggtaa cttctgcatc cttctgcgct tttgccacta
     4261 caataaagct gacctgaacg gcactggatg tagctgcatt cggcgaaata gttatcgtca
     4321 gagttccttc ttcgcctacc tctacatttt catacgtaag agtatcgttt acattattct
     4381 ggtaattgta tttctctgtt actaccttat cattcagact gatatccgca tagcgttttc
     4441 catcccacca gctggagggg tcaaacatac ccacataaac attatatttt ccggcttcca
     4501 gatcaaaccg ataactgatg ctctgggtct tgtctgttgc ataacgcata ctttgataaa
     4561 tgtcatctgt gctgttacgc agctttcctt ctgctcctac atatccaaat cctgtttctg
     4621 tactgtaggc gccatcatat acatccactg tatgtaacaa tgtattgccg agctgtttca
     4681 tgatggaagt ataatctgca cttaccggtg ctttcgatgc atttgcaaaa taaactgcat
     4741 ttttcggaca taccagcatt tctgttgaga tctccgcgtt ccggcaatcg atgagtttac
     4801 cggtgacagt ggttctgccg atgacctgat aagaactctt gtcccaggca accttactct
     4861 ccacagtctg tcccttatag cttaccgtga ctttctccgg caaattactg ccgtccgtcc
     4921 atgtgatctt gggaagtgca gtctgtatgc caaccgggat cagatcttcc agttcttctg
     4981 tcttccagtt ggtgcgattc agaatcgcaa tagtatggga agtggcatct acctgtaagg
     5041 gcagccagac agtacggctg tcggacaatt tgttgccatt ccaacggtct cccatataaa
     5101 tgaactttcc tgcaggtaca tcaatcggaa taatatatgt cacctgtgta tcaaaacatt
     5161 ttccggtatc attctttgcc gggtttccta ctttttccca gccgcttaga atgttctgac
     5221 tgcgatagta ggtatgtgcg gtgctgttcc agccatccgt tccgctcgta atcatgtaat
     5281 accaaccatc gtatttcatg acagcgggtg cttctctgct attgtcagat accagacggg
     5341 ctgccatctg ttgattgtcc gctgtattac ccttggcagc gagcccggta taatcactgt
     5401 tcaatagaga tgcatacagc tttgcattca tctcactgga gtagattaca taagcatcct
     5461 ttgctccgtc tgcatttgca tccacatcat cgacaaacac agtcatgtct ctggcttcgc
     5521 ccaaccgctg tgcattcagg ctctcatcat aattcatacg ggtcatattt accacttcga
     5581 aaggtccgaa tggtgtatca cttgttgcaa aaccaaccat agcgcggctg tatctgctgg
     5641 cttcacagtt gccctgcata ccgttcttca cccagttgta cagcttttca ttgttgtaca
     5701 aaggtccatc tgcatggaaa ataatgacaa atttctttgt cttttcatta tagaccatct
     5761 taggtcgctc agtaatgcaa taattaccat acattccctc gaaagcaagc tgtaatctgg
     5821 atacggtctg ggttccattg gtcttgctgt cagggtacag gaagctacct gcctctacct
     5881 ttgtctcatc ataactcttt gcaatccagg atatatcctc agcgctcgtt ggctctttct
     5941 caaactctgt gacataggct cttaagaaca gcttgacatc acggaattcc gcttcggtga
     6001 caccctctgg tgctgcggac agctttcccc atgccttcag agtctcgaaa ttggtattac
     6061 tgagctgcat tgcaggataa ctctgggtcg ttcctgttcc atttgctcct gcggaggaag
     6121 tgattgcttt ctctgcggat tcttcaatcg gcaggatgct gctctgaaga tacagtactg
     6181 ttcccttgtc ggtccagttg taaagatcgg tagaaacata acattttaca ccatctaccg
     6241 gtctggtgtt gttggtctta tcttctccat accacagata tacggtctta ccctctgtga
     6301 tctgtccgtc accatcgagg tcaatattga cgcatccctc tccggttcct tctttcatgg
     6361 ctgctgcgga accgccgtgt gcctgcatcg cctttccgtt cgtatcacgg atgacactgc
     6421 ctgatgtacc gggaatactg tcattcggct gaatttcctc ggcatctccg gtccgactga
     6481 caacgagcca gctgatgaca ggagcctgtg ttccggttgc agtgatcgta taggttacgg
     6541 tctgtttggt tcttacgtca aaagaataag tatttacagc attcggattg ctgccgtcta
     6601 cctgaatcgt tccggcatcc agtgtaatat tatccactgt tactgtcagg ttcatcggtc
     6661 tggtcattcc ccaccactct ctgtgtgcag aggtaatggt gtatttaccg ggatctaaag
     6721 tcagcgcgta ggtcagagtt tcgcctgatt cgttattgtt cccgtaaata ccggttgctg
     6781 tcttatctgt agtatcggtg taactcttgg ttcccgcgtc agtatctacc agcccccagc
     6841 tattgccgga ggtctttaac tgatccgcag tctggttcaa cagcgcactt cctctgagtt
     6901 ccttcactgc tgcaaaggct tccgtttttg catctgcatc cggagttccc tttaccatat
     6961 caataaaata taccgtattc tccggcacca cttctaccgg aatcgtcact ccgagacttg
     7021 ctccgaagct gttgacacca tcctgcacca caccggtgac actggtcgtc gcataagctt
     7081 ccgagaaatg ttccgccgta ttattgctcc atacgatagg cttggtcact acagttccat
     7141 ttcctgtttt tacttctacc gtagccggca aattcttgag ggtctgattc tgctcaactg
     7201 cataagctgc aggaaatttt gtttctacgc tgacaagcac atcatcaccg atctgggttg
     7261 tctcacccaa cgcatacagt gcatctattt tcagggttcc actctttact actactttga
     7321 tattatgatc tgcaaggctc agaccactat agctgtaaga ctcataacga cttgcagctg
     7381 ctgtggtcga aacattctcc gctacaaggt tgttatctac atagacatcc agcttcgtcc
     7441 cggcgttgtt aggtccaata attgcaaatc ccgcaccatg gatagggaaa ctcactgttg
     7501 caccgcttcc gcctgataca gacaaggtac gataccagtt gtccgcactg ccgcttccgg
     7561 gattgtcgat tgtccaactt ccctcatagc ttacgctgtc atcctgtcca tccaccatag
     7621 acaacgcata agcacatcct cctgcaatct tctcaatgag aaggttatca taatatacct
     7681 gatgccagtt gttgctgatc ttgacgcgac cggacagcat cggagatgcg tcttgatagc
     7741 ttgtgacctg ttctccgttg ataaacgcgg taatcatgtc gccaagggct gccagcttta
     7801 attcatattt gccatctgtt gtggctgtta ccgttccatt tgctacagca ctgcctatgc
     7861 ggtacagctt ccaagagccg gcaccgttaa tctccagcgt atagccgctc tggttccagt
     7921 tcatgcctgt ctgggtacgg atacccagtc tcgcataggt cgatgcagat gcatccggta
     7981 tctgcacatc tatcgtggta acatagtcca tccaacggaa atcaccgacg atcgttgaag
     8041 gttctccacc gttccactgt cccacactgg tcgtcagttc cgattttaat ctgccgttct
     8101 ctacaatgta agcgccatgc gtatccaaca tgtaacgtgg ctcattgcct cttgctgtca
     8161 gataatctac actggtggtt ttcccggttt tggcattata ctgttccatt tccggttctt
     8221 ctgtatactc gaaatcatcc gcataaagta cattatccgt tgtattctga ttgcgtccgg
     8281 tactgtcagt atccaaaacc gttctgtcct cgttatggat ttcttgttcc ggtgttcttg
     8341 caggtgacgt attcagtgta gttgctgtta ccatcccata agccggaact gttacatacc
     8401 agcaaccgtt ctgctgttct gcatctgtat cactcttttg taaatagcta tctgttgtgg
     8461 ttgtccatat ttttaaggtt gcatcggagg ctactgccat atcctttgca gtaatcttaa
     8521 aggttttttg atttcttgtg ttatttacaa aaattacaga aaagtctttt ttatccggag
     8581 ataccagtgt catatagctg gctttcccat ccatccctgc tgtctgatgc tcattgtcac
     8641 tggagccgaa ggatccggag gttgcaccgg tgataacacg ccagatttca ttctgggaag
     8701 ggtcggtatc ctcccatccg gtgactgcaa actgagagaa atgtgcaatc atgtacagtg
     8761 ccggatcata atggatataa ccgctccagg gatctctggc acttaacagc tctttatggc
     8821 catactggat gccttcatag aaggaaccga tagccggctg gaaaatatag tgggttctgc
     8881 gagatccggt aaacgcattg ataaagctgt ctgccagagc caacggactc tgatatcctc
     8941 cgatcgttcc accaccgtat tcactcgtct tgttttcctg taactctgta taaccaaagg
     9001 ttgcacaccc ctcgctgtac catacctcct tgtcgtccac atccgccatt ttgatgtaat
     9061 cctccgtggc actggtacga taatggaaac caatgatatc tactgcattg tagagatcat
     9121 tgtcgcttct catggaaggt acgatttgca gggtcttatt ctcgtcagaa gcaataattc
     9181 tgatattgtg ataagcgtca atcgctgcct gatccatata gtccgggaaa tctgtttctt
     9241 tttttacccg gttggaaaac catttgataa agtcctcatt agggtctgtt gtctcgttct
     9301 tatcaggatc tacgaaatct acgacataac catatttttc ataggcatca aaaatggtct
     9361 ccttgtacca tttataaact gcttcatatc ctgcaccggt acggtcactg ctccatttgg
     9421 aagcaaccca tgccggcatc tcccaacgca aaatactgat cttgacattc gggttgatct
     9481 tcttcgcatc ggctgccatg acaaatcccg gagagcggga agcatccgct tcttcgttct
     9541 cataacgcat ggtacaggct tctgctccgg tagaattatt tccgtcattt cccatttcca
     9601 tcttgatatg ggtaaacaaa ggatattctc caccgaaaag ataccgcatc atttcccagt
     9661 attgatctga atgctctgcc ttgtaatcca gcagcaggtt actggtactg ttaccattta
     9721 acataccaaa gcccttataa gtcagaccat tgatattggc tgttgccttg tcaacatctg
     9781 caccggatat cactatgttt ccgtctgtct ggacagcgcc tttgatggca tccagtcttg
     9841 cctgtgtttc cgtaatggct tcttcctctg ttgctgctgc tgccatgaca acaatattgt
     9901 cataagtacc gttgcacaaa tagctggttc cataagctgc accgattccg gcagccagat
     9961 tggtagtatt acttaattta aaacctattc cagtggcatt cagtaccttt gctccgtcac
    10021 agtataatgc cacatagcct tgctgttcat cctcggtata cacaatagaa agcgcatgcc
    10081 agtcccctgc agtaagagcc gttgtcagag gataatcctt tgctcctgca ttggtaccca
    10141 ctctcagcac agactgactc tgggactttg ccggaattac acggaaagca gtggaatcgg
    10201 aattaccaag gaaaacagca gcacgactcc ccgttccttc ttgtatgtaa ttgtatttta
    10261 agttgagatt atatgtaaac tgatttctct ggaaaaaatc aggtgcattt tttatggttg
    10321 cgcctgcctt agagatacca ccactagtcc agccacctgt cacagaaagc tgctttcctg
    10381 tgccgctgtc tgcttctaag gaagcatcag aaagaacagt gagctgatta ttgtcttcca
    10441 gagcctgtcc aaaatattcc gattcctttg tgatatattt tgatactcca agccagctta
    10501 aaacaggagc ctgctttgcc gtactcttta aggtataagt aaccgtctgg gcagtatcca
    10561 gcgtaaaagt ataagaaaat tgtgctgttt ttacactttt gctaacagat acacttccaa
    10621 tggttgtttc tcctgcgtct gtttttaaca gcacatccat aggacgggtt ttgtcccacc
    10681 attctctatg tcctgaagta atagtatagg tgcctgcctc cagatacaga tcataagaca
    10741 acgtttctcc tatggcatta tttccataaa taccggtggc tgttttatca gtagtcgtat
    10801 tgttatattt tttggttgtc gcatccgtat ctaccagacc ccatgcagta tccgccgtct
    10861 tttgctgatc atagacttta tttatcagct ggtcacctgc taatgtcttt actgcttcat
    10921 aaggctctgt tgtagtttcc gaaaagtctg atgttacatt gtcaataaag tatacaattc
    10981 cttccggtac aacctcaacc gtatagctgg ttccgtccgc cgcctttgcc gtaaccacgc
    11041 tgtacgactt gctgaaagta tctaccgtca catctgcagc ccatgtgatt ccttccggaa
    11101 tttttcctgt ataagttgca atatgtgtat tggaagcttc caggcttatt aatgcgattc
    11161 cgtctccttc tgatacggaa tccgcctgtg gggcaccact ctccgttcct gtttcttctc
    11221 tctcctttat aactacgttt ccatcagata cagaacctgt ggattgcgta ttgccttctg
    11281 ttccggcttc tgtatcagtc tctgtgcccc caacaggctg ttctgactgt acatccgctg
    11341 ccaatgcatc tatcggcacc gaacctgccg tcagacctgc acaaagcaac agagtcaata
    11401 ctgttttcgc tactttacat gttttttgct tcatacaatc ctcccataat tatttataac
    11461 aatacatctt tattataaaa aatgagacag atgtacaaaa tgcaataaat tactttttac
    11521 ttcctttttt ctctatgatt tttatgatta tttttgtaag tgtttacata taatgtaaaa
    11581 aattatgcaa ctatatactt ttttaatatt tatgtttcaa caatttacag tatacataat
    11641 acttttattt catataaaaa caatggagcc tgcaaatagc aaaactccac tgcaatttct
    11701 atatattcag attacttccg catccactct ggtgacgatt tcctgggatc ccttgcggat
    11761 gtggtatttc actgccggga tcttggtctt ggtgtagagc agg
//
LOCUS       DAWEJW010000086        11762 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1920_length_11762_cov_67.358589, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000086 DAWEJW010000000
VERSION     DAWEJW010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11762)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11762)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11762
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1920_length_11762_cov_67.358589"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2867..2966
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3120 a         2622 c         3023 g         2897 t
ORIGIN      
        1 aggaagtatg acgacttcaa tcagcgagat ccgtaaggat tttcctctgc ttatgagtaa
       61 cgatgtgatt tatctggaca cggcggcgac ttcccagaag ccgtcctgtg ttctggaggc
      121 agagagaaca ttttatgaaa aatacaatgc caatcccctg cgcggattat acgagctggg
      181 agaggcggca accgaaaaat acgaagaagc cagagagacg gtccgggagt ttttaaatgc
      241 cagagaggcg gcggagatcg ttttcgtccg caatgccacg gaaggcttga atctggtggc
      301 ctacagtttc ggctcactgg tactgaaacc gggagatgag atcctggtga gcattatgga
      361 gcaccacagc aatctcctgc cctggcagca ggcggcagcc agaaccggag cagtggtgaa
      421 gtatctggag tgcaccgaag acggaaaata taccgaggaa gcttttaagg aggcactgag
      481 cgaacgaacg aagatcgttg ctatgaccca ggtatccaat gtgctgggct gcaaaaatga
      541 tatcaagcgt tttgcgcagc tggcacatga ggcaggcgct tatttcgtgg cagacggagc
      601 ccagagcgtt ccccatatgg cagtggatgt acaggatctg gatgtggatt ttttggcatt
      661 ttccggacac aagatgtatg ctcccatggg aattggtgcg ttatatgcga agagagaact
      721 gttggagcag atgcctcctt tcctgtgcgg cggggaaatg atcgattccg tcagccgtac
      781 cggagccgtg tgggcggaag tgccacataa gttcgaagca ggaacggtaa acggtgccgg
      841 agcctatgca ctggcagagg caatccggta tgtgcagaag atcggttttg actttatcga
      901 agagagagaa gcgaagctga cagccatgct gatggatggc atgaaagaga ttcccgcagt
      961 acacattctg ggctcacaga aagcagaaga ccatcacggc attgtgacct tcaaactgga
     1021 cggtgtgcat ccccatgatg tggcttctat tttggatgcg gatcatatcg cggtccgggc
     1081 gggacatcat tgtgcccagc cgttattgca acatttgaaa gtattgtcca ccaccagagt
     1141 aagcctgggt gtctacaata cggaagaaga gattcaggca tttttagaaa gccttgcaaa
     1201 gataaggaga cagatgggct atggagaata agacatttta taatgaaatc ctgacggagc
     1261 acaatctgca tccgtatcac aagcacaagc tggaggatgc aaatctggtg ttggagggcg
     1321 ttaatcccag ctgtggtgat gacatttttc tggagctgaa agtggaggat ggtgtggtag
     1381 ttgacggtgc cttcgaaggc gacggctgtg ccatttccca ggcttccgcg gacatgatgc
     1441 tggagctggt cattggcaga agcaaggagg aagcgttgca tctggcagac ctgttcctca
     1501 agatgatcaa gggaagtatt accgaggaag agcgggacga actggaggaa gcctccgtat
     1561 tgcaggatat cgcccacatg cctgccaggg taaaatgcgc ggtgcttggc tggcatacta
     1621 tgcaggaatt attgaaaaca gaagaaaaat agctgataaa aagataaaga tacatcttgc
     1681 acgccctttt tggatacgct acaatgaaaa aagaagtgta agaaaagcgg ggggctgtgc
     1741 atgttccaca tagagcttat cattgaattc ctggaaaacg cgatgaacaa tattgtccag
     1801 gtggcaatac tgttctttga atttgtcgga gtgatcgtta ttattctctc cggtctgaaa
     1861 gggttttata attatgtgcg gcgggtgccg gatatgaaga tcaccctggc gcaggggctt
     1921 gctgtagggc tggaattcaa gttgtgcagt gagatcctta agaccgttat tgtcagagac
     1981 tggaaggaga tcgttaccgt agccggtatc atcggacttc gtgcggcact gactttcctg
     2041 atccattggg agatcaagga agagaagaat cattcctaaa aatcggcagc tgatgaaaat
     2101 cggctgcttt ttttagaatg atttccggtt acccaacaaa agtcaataac tttatccaga
     2161 taaagagatt aaatttgtaa tggtatatag gttaaatttg caccttggaa aaaagctgtc
     2221 agaggactaa catataacga taaagtatgt atttataaaa aagtgtaaag aaatgctaaa
     2281 aaatccgttg catttgacac ttcgacgtgc taaagctatc gatagcatga acgtaaatgt
     2341 aatcgattac gcatgcttaa aaggaaagca gaatggaatt acaaaagaca cttactcccg
     2401 gggaagcgct ggaacgcgtc ctgcgatctt atcagaccta ctacaatatc aaaacagagg
     2461 ctgtagaacc gccattcgca gccgaagcaa tcttcggatc ccataatgag cagtactttc
     2521 tgatcaagaa agctaaggtg gcagatattg acaccaacga gactgtgtat tttgccacag
     2581 aggaatcttt atcaaaggat cgccttctgg aactggactc catcgcctgg gaacggggaa
     2641 ctgccaacgt gcagccctca tccaatcatc ggaacagcga tgttgtcctg atcattctga
     2701 ccggtcatgc agaggaggat gcgcttacac aggtgaaaaa gtgcaagcat tatcagagtt
     2761 acctctgggg atttcatggc tggagtaatt accgtttgat tgtcgccgag ttatcttcgg
     2821 gtcggatcgt ccacaaccgc cacggacaga ttctgaagaa gctcgtnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnaaat ctgtgtataa gaaaaggaga aacacaaatg
     3001 acagcattat taattgtttt ggccgctatc gtattattgt ttaccggcta tgtgttctat
     3061 ggctcctggc tggcaaaaca gtggggcatt gatcctacca agaagactcc tgctattgag
     3121 aaggaggatg gtgtagacta tgttgcagca aagcccgccg tacttatggg acatcatttt
     3181 tcctctatcg ccggagcagg cccgatcaac ggaccgatcc aggcttccat cttcggatgg
     3241 gttcccgtat tcctgtggtg tattatcggt ggtattttct tcggcggtct gcaggacttc
     3301 ggatctttat tcgcatctat cagacatgac ggtaaatctg taggtgagat catcgaggat
     3361 tccatgggat ccagagcaaa gaagcttttc atcatcttcg cacttctggt attgattctg
     3421 gtaatcgctt ccttcgttaa catcgtggca ggtactttcc tgactgtggc agacgaagcc
     3481 ggcaagaccg ctttcggttt tgtgaccaat cctaccggta accagtctac cgctatgatc
     3541 tctttgttat ttatcgttct ggctgttatt tatggttatg tgaccaacag aatgggtgtc
     3601 aagactctgc ccgctaccat cggtggcatc atcggtatcg tactgatcac tgttcttggc
     3661 ttgaacgttg gttttgcaat gaaccgtatc gcatggatcg tattcgtagg tgtgtacatc
     3721 gctgtagctt ccctggttcc cgtatggatc ctgttacagc cccgtgacta tctgtccagc
     3781 ttcctgctgt acgctatgat cggtctggca ttcatcggtg tggttgcagg tgctttcacc
     3841 ggtactgttg ctttcgatat tccagctttc accagctggg agcccaaggc aggacagacc
     3901 ctgttcccta tgctgttcat cactgtagca tgtggtgcat gttccggttt ccacagcctg
     3961 atcgctaccg gtacttcttc caagcagtta gctaacgaga aggatgccaa ggcaatcggt
     4021 tacggctcca tgctgatcga gtctgctctg ggtatcatcg ctctggtagc agtcggtgca
     4081 gtatatcaga agtacttaaa cggtgagttc ggatctcccg cagctgcatt cgctgcaggt
     4141 atcgcatcca tgttcggtac cgagacttcc aaggcatatg gcaccattta cgcactatta
     4201 actctgtctg tatccgtatt cgcgctgacc agtctggata ccggtacccg tctgagccgt
     4261 ttcatgttct ccgagctgtt cctgaaggaa ggcgaggcta cctacaagga tgccaagggc
     4321 gcacgtaaag tgctggctca tcctctgttc ggtactgttt ctatggtact gatcggttgt
     4381 atcctgggtg gtctgtctct gagccagatc tggggtctgt tcggtgcagc caaccagttg
     4441 ctggcaggta tcgcactgat ggcagtagct gcatggctgg gtgaagttgg taagaacaac
     4501 aagatgttct acttccctat ggtattcatg cttgctgcaa ccctgaccag cctggtgatc
     4561 actgtaatca acaagtgtaa ggctatcggc gccggcaccg ctgcatgggg tgactggttc
     4621 cagctgttct tcgcaactgc aatggcagtg ctggcaatct tcctggtagt ggaaggcgca
     4681 cagaccttcg ctaagcagat gaaagagaag aaggctaaga aggctgctta atataagcgc
     4741 aagatacaaa agataaataa agttaagcaa agagaaatcc cggagactgt gaaaggcctt
     4801 cgggattttt attgtgtatg cggggaccgc agaagcggtg tccggaataa taatggatgc
     4861 ggcaatcgca atatcagccc cgcgtaagga accggggcaa ttcttcaatg ggaatcggct
     4921 tggagaactt atagccctgt aaataatctg catgactgat gatacacagt tcttcctggg
     4981 catcggtctc tacaccttcg tacagtacct tgtaacccag acgctcaaag gcggtagaga
     5041 agtaatccag catgaagcgg gaattggcat cctgatccgc catcagtaaa aggcttctgt
     5101 cgaatttgac cacatccagt ttcaggctta agatccggtc gaaattggaa taaccggttc
     5161 caaaatcatc cagataagta cataccccca actgtttgaa ctcactgacg cattcctgta
     5221 ccagttcgta ttccgtatcg ttctgggact ccgtaaactc aacggcaatg gtgtggaagt
     5281 cgattccggc ggctcgtacg atggatttga attcttccat gaagtccttt tcgcccagct
     5341 cttccacaga aagatttacg gatacacggg agatctgata gccctcatct tgcatgtgct
     5401 tgatctgcct acaggtctta ttcagaatga tcatgctgag gcggtggatg tagccgtatt
     5461 tttctgccag aggaataaaa cgatccggga ataccagtcc tgtctgtggc agacgcaaac
     5521 gcatcaggga ttctgcggta tcgtaagtac ccgtgtggac attccgtacc ggctgacaat
     5581 agaccagaac gcgttcgtca tctaaggagc cgtgttccgc aatatccttc aactgggact
     5641 tgatataatg catttcttta taagtctggt aatcttcttc cccgaagaca cgataggaat
     5701 tctgcgctac tttttcggaa aagtaattga atacttcgta gaactgttcc agattctcac
     5761 aaaagtccag atggtcgaag agtaccagct tgtagggaag cttatattct tcataatatt
     5821 tctggaacac ctttttaatc agggatactg ccctttctgt ggcatccggg acattgtggc
     5881 tgtccactgc cagtacgaag aagctggtgg aaggattgaa caacataccc tttcgaaaat
     5941 aatccgtcca gaagctgtag aacagctttc ccatttcctc tgtcatgacg tattcgaaat
     6001 ccatgtcaaa gcgcaggcac aggtagtagg tatcctgagc ggtctgtcgc tgctggcgca
     6061 gatattcatc caaggatgtg gaacccaggg caccggtctt cgggtcgtag gcgttggcat
     6121 ggagcatgta caataccacc agaagcggca gaaaataggt tgtggccagg aaagaagtcg
     6181 tattcatggc agcttccatg actacaatca atccacagac cgtctcagta aatatcagca
     6241 tatggaacag tgcggtagga agtctgcggc ggtagcaaac caacagaaag aggatcaccg
     6301 ccagatacag aagatatccg accataaaag gagtggacag gatattttca taccagattc
     6361 cgtaatgatc ctgatgaaat ccccagccgg tcagaggaga tgccagatcg gcaactccgg
     6421 tcaccacaag gatggcgaag gacagaaagg tatatttcct gccgatattt ttcgggaaga
     6481 cgatcagtat tttcaggtaa gcaatataga gacagaacag ggacagaaga ctgatatggt
     6541 agacggcccg gaggatccag agaagggtgt tctgtctgga aaaaaggtcg gcataataaa
     6601 aggcaatatt ggaaactgcg gcaatgaaaa gcaaggcaaa acaggacttg aagatccgaa
     6661 aggtccggct ctgtttaatg aaagtctgtc ggatcagtaa caatgccaga agacacataa
     6721 ccaccaacag aaaatctgcc acgggagagt aactgtggat gacgatatga tttgtggata
     6781 gtaacaggtt caaccccata aaaattctcc tctctggtaa tgtagaaaat gaacaaacaa
     6841 tataaaatga ttttacaata taccataaga ggagaaattt gtcagtgggg cagctgtggg
     6901 aaattagtcc tattttgagc aaatgggaaa aattgtaata gatttaatgt tcgaagaatg
     6961 caacggcaac agcacccggg cccacatggg ttccgatggt ggcacccaga gtgctgacgg
     7021 gaagctcctt ggcataatct ttccagagat gctcactgtc gtgaatgtat ttctgtaaaa
     7081 gttcgtcact cagaccggta tagcccagac agaagggctt ggagaagtcc acaccgtccg
     7141 ctttttcaat ttcacggatg aggaagttac tgccgttttt ggaaccacgg gcttttccca
     7201 gcataatgat ctcaccgtct acgactgcta ccacgggctt gatggccaga gcacccccga
     7261 tgaatgccac agtgggggag atacggcccc cttttttcaa gtattccaga gtgtctaaga
     7321 gtcccatggt gtggatctta tgcttttgct cttgcagaat gcttacgatc tcttccgcgg
     7381 tcattccctg ctttttcagc tgcaggccgt attctaccag aatctgttcc ccaatggtgg
     7441 cattcagact gtctacgacg aagactttgc cttcataatc ctcggcagcg atccgggcac
     7501 tctggcaggt gccggacagc ttgctggaaa tagtaaccac aatgacagtt tctccggccg
     7561 atacagcttt ttcgtaggct tcttcgaaga ctgcgggaga taccagactg gtggtgggca
     7621 gagtatcaca ttcgatcagt ttctcataga attccttatg actgatggtg acaccgtcag
     7681 catattcctc ctcaccgaag cggatatgca gggggagaat cgtgacgtcc tctcttgaat
     7741 tgttcatatc ggatgcggag tccgtgataa aacgtatttt ttccataatt gtttcctgta
     7801 ctttctatat atagttatga tgaatattct ttcgggcgga aatgcgccgg ggcatccatc
     7861 gggatggaca tacgacatcg ttcatgtcac cttgctgtta attctgttgg aaagccggtc
     7921 agggtgagtt gcggatggat catttctctg tgggatgctt gacagaaaac tctgtaagat
     7981 actgctccag attatgggag atccgttcca gagagtcata gagattcaac cgttgttctt
     8041 ccgtcatgcc atcaccgccg tgagccagtg cggaatagat ccgggaactg atctttttga
     8101 ctacagtatt gccttcttct gtcagataat acagtgtgcg gtaggaacgt ttatgttccg
     8161 gcagctcgca ggacaccagc cccttggaac tcaactggct taaggtacgg gagatggcgg
     8221 ccttgtcttc cttacacagc tccgtcagct gcgtggcggt cagcccttcc gggtgctttc
     8281 ccagataata cagacacatg gtataaccgg cttttaagtc catctgtccc atttccagtt
     8341 cttttaattt ttgcagataa cggtgcaatt ctgtgataga taccacaaat ctttcaaaac
     8401 gatcctgcat gaaggttcct cctgtaaaat gttgacactg caacaacttt atgaagtgta
     8461 acacaaggat gttgataagt caacaaaaag aagggcaaaa aaatacctta tccattaaga
     8521 taaggtactg ataagaataa actatgcaat ttccagccgc tgctttattt ttcggacatc
     8581 attttccaat acattaagcc ggacgcgaac cagttcatca tcttctttta cttttaaggc
     8641 ccggtcgaat tttctggata aatctaaatg agcttctgca attgtacaga tattacgact
     8701 gacttcattc tcaagagtca actccatatt ggtaaggcga ttcgccatag agagctgacc
     8761 ggattccaga ttggcaaggc gctgttccat ggcaatctga ccggattcca gattggtaag
     8821 gcgctgttcc atggcaatct gaccggattc cagattggaa acacgttgtt ccaaagaatc
     8881 gaatctttgc tccagtgaat caagtcgctg ctccaccctg tcgagtcgct gttcgacctt
     8941 atcaagtcgc tgctccaccc tgtcgagtcg ctgttcgacc ttatcaagtc gctgctccac
     9001 cctgtcgagt cgctgttcga ccttatcaag tcgttgctcc accctgccca gtcgttggtc
     9061 tatactttct aatttaatta ctatcgtctc taataattcc cggttggtca tgaattctcc
     9121 tcctgtacat gtatactagc acagttcaaa agtgctgaca attgggcaat atagagaaaa
     9181 aagcgattgt gcattattac aacaaaaaat atgataaatg gatgataagc cccaaaaaca
     9241 tgatatatcc aaggaaaaag agtacggcaa aataaacaat gccgattgga acggtagaag
     9301 agccagagag agcggctgcg gtatggattg tactgcgtat tcgtttatgc tatgctaaat
     9361 agcagaagta ttccggaaac gggaatgcac aatgtgcaaa aagcataatg cactaaaatc
     9421 acatggtgtg atagatcgca ttgaacatag aaagagtgag gactacagca tgctcagacc
     9481 cagcatcggt attgattggg acgatgttac cgccccgttt aacagtatcg ccatccgtat
     9541 ggcaaatgaa aaatatcatc cgaaggaacc ttatcgcatg gaggaaatca cttcctgggc
     9601 caacgaaggg cggacttcgg tgatcaaaga attctataac gatccggaac tgtaccggag
     9661 gcagatcccg accgaggaga cgaaacgagg gatccgcaga ctgatgcaga tcgcggatgt
     9721 ttttttcatt actgcggtgt caccacattt tatgggagtg cgggcagaac agatcatgac
     9781 ccagttcccg gagcttccac cggaaaatat tattctggga agtgccaagg atcgtgtcca
     9841 ttttgatatt gtgctggatg atgccatcca caatattctg gagagcaagg cggagtatcc
     9901 ggtgctgatg cggaagccct ggaatgccaa aatgacgggg ctgttgtccg taaataccat
     9961 ggcggaattt gtctctctgg tgaagcagat catgaaagca tccacttcta agacagagaa
    10021 gatcactgct ccggcggtgc tggccctggt ggggccgtcc ggctccggca aacgggagat
    10081 tacggaagct ttgtgcggta gccgcgggac aggcacaagc gaaaggacag agagtgggga
    10141 gatttttgtc cgcccggtga actactgcac ggaaccgggg cgctacggcc acaagtacgt
    10201 cccggaggaa gccttcgacc ggatgaactt ttttgaaaaa acggcttatg ccggggtacg
    10261 gtacggcacc agaaaggaag acattcagac cttgctggat cagggaaagt ttgcggtgat
    10321 ccccgtggat atgtgcggtg ccattgccat gaagcgttcc tttcccaccc atattatcta
    10381 tgtggcgaga gataaagaaa agctgatcgc cgatatcatt gattccgact atgacaccga
    10441 ggaaaagaca cttcggatcc tgtctatcga tgcggagaag cgtaatcgca agatctgtga
    10501 ttatgtgatc cataatgata ccatagaagg ggaacgtgtc agcggagcgg aagaaatccg
    10561 gcggctgatc ctgctggaag gcgaaaaata ctgaaatgta ccggggagcg atacccggaa
    10621 aacggaatag agttgtacaa aaccggatag agaaggagcg gggcggacgg atacgtatgg
    10681 aagaaaaaca gctgaaatac tataaaatgc atcatgattt acaggaacag atccgactgg
    10741 gagaactgcg cagcggtgac cgtgttccct cggagaacca gctggccgcc gcttatcagg
    10801 tgagccgcca gaccgtccgc aaggcactgg ccattctgga acaggaggga tacatctatg
    10861 cggtacacgg gaaaggcacc tttgtgtcgg agcgggtccg cccggagcat aagtctcaca
    10921 acattgccgt ggtgaccacc tatttgtcgg attatatttt tccccgggtt attcagggca
    10981 tcgatgaagt tcttacggca caggggtata gcattttatt aaaaaataca agaaactccc
    11041 ggagccagga ggcgcggtgt ctggaggaac tgttgcagaa ggatatcgac ggggtcatca
    11101 tcgaacccag taagagccag atcagctgca gacatctgca tctgtatgag cggctggaag
    11161 aatacggaat tccctatgtg tttattcaag gctgcttcga ccagatggaa gacaagcccc
    11221 aggtgctgat ggacgattgc aggggaggtc atctgatcac aaaatatctc ctggataccg
    11281 ggcatcgtga tattgccgga gtgttcaagg cagatgacat ccagggacag aaccgtcacc
    11341 ggggctatgt acaggccctg caggaagcag gtgtcctcta cgatccggac aaggtgatct
    11401 ggttccatac cgaggatcgt aaagtccacc cacaggaggg aatccggcgt ctgatctcca
    11461 agggaaccag actcgacagt gtggtctgtt acaatgacca gatcgccatg caggtcatcc
    11521 cgacgctgac ggcccggggg attcgcgtcc cggaggacat ttccgtcacc ggttatgaca
    11581 attcctacat ggccatcggc ggaggttttc aactgaccac tatcgttcat ccccaggaaa
    11641 aactgggaga aatggcggca cagctgttac tgtcactgct tcatggggaa tctctgggac
    11701 cggaagagag aaagattctc atccagccgg agcttgtcgt aggaaataca acttgttagc
    11761 ag
//
LOCUS       DAWEJW010000087        11659 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1938_length_11659_cov_64.247759, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000087 DAWEJW010000000
VERSION     DAWEJW010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11659)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1938_length_11659_cov_64.247759"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1661..1760
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2785 a         3016 c         2471 g         3287 t
ORIGIN      
        1 tccttgtttt caaacggata atgatgctgt gcaaaaggca ttgctccgga gtcgaaccaa
       61 cagtcgatta cctcaggaac acgcttcatc tgcttgccgc actcggggca ggtgatggtg
      121 atctcgtcga tgtaaggacg atggaattct acggtcttcg ccttctcatt gccgctcatc
      181 ttgtacagct cttcacggct gccgatggaa tgctgatgtc cgcattcgca ctcccagata
      241 tttaacggag taccccagta acggttacgg gatacgcccc agtcctgaat attctccagc
      301 cagttgccga aacgtccctc accgatagaa gcggggatcc agttgatggt cttattatta
      361 cgtaccagat catccttcac agcggtcatc ttgatgaacc aggactctct tgcataatag
      421 atcagcggag tatcacatct ccagcagaag ggataatcat gctcgaactt cggtgcatcg
      481 aacagcttgc cctctgcatc cagatcttta agtaccagag gatctgcatc ttttacgaat
      541 tttccggcat aaggggtctc tgcggtcaga tcacccttgc cgtctacgaa ctgtacgaag
      601 ggcagctcgt aattacgtcc gatacggctg tcgtcttcac cgaaagccgg tgcgatatga
      661 acgataccgg taccgtcggt catggttaca taaccgtcgc aggttacgaa atgtgccttt
      721 ttcttctgct tcttggcggc atctcccgca cacttgtaca gaggctcata ctccttgtac
      781 tccagatcct taccaacata agtctccagt acttcgtaag catccttgcc ttcttctgca
      841 agacgtccca gaaccttgtc tgccagtgcc tttgcaatat aataggtata accgtcggcg
      901 gccttcacct tcaggtaggt ctcctccggg ttcacgcaca gtgccacgtt ggaaggaaga
      961 gtccaggggg tggtggtcca tgccaggaaa tatgcatcct cgccctttac cttgaaacgt
     1021 acgatagcgg aacgctcttt gactgcctta tagccctgtg ccacttcgtg gctggacagc
     1081 ggggttccgc agcgagggca atagggaacg atcttgaagc ccttgtacag aagtccctta
     1141 tcccaaatgg tcttcagtgc ccaccattcg gactcgatga agttgtcatc gtaggtaaca
     1201 taagggttat ccatgtcagc ccagaaacca acagtacggg agaaatcttc ccacattccc
     1261 ttgtatttcc aaacgctctc tttacatttg tcgatgaaag gtgccagacc gtattcctcg
     1321 atctgctcct tgccatccag acccagcaac ttctcaactt ccagctctac gggaagtcca
     1381 tgggtatccc agcctgcctt acggggaacc atatatccct tcatggtacg gtatctgggg
     1441 atcatatcct tgatggtacg ggtctctaca tgaccgatgt ggggcttacc gttggcggta
     1501 ggcggtccgt cgtagaaggt ataggtctcg ccttccttac ggtgttccat actttttttg
     1561 aaaatgttgt tgtcttccca gaatttctcg acagctttct cacggtctac gaaattcagg
     1621 cttgtgggta cctgcttgta catagtgcgc ttcctttctt nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn cgtcccgtaa caggacgaag ctcatgcatt cgttaaccac
     1801 ctcttacaac atacttccca tacgattcca catgtgtcat ctcacaggag tgatatgctt
     1861 cccggtaacg gcgggattcc gttcagaact actgacctgg gcttttgttc tgacagctca
     1921 gaagtgattt tcactttttc cctactcgca ccggtctctc accatcaccg gctcgctttg
     1981 cctttctgcg gaaaagttac tctcttcgtc aatgcatttt tgatataccg ctattatata
     2041 tctcattttt ttgcgttgtc aagccggatg ttatgatttg cttagaaagc ttttctttct
     2101 tgcttttccg acatttattt catataatta ttttaagact gatatgtgta ccgatcttac
     2161 cggaaagggc agggaattct atggaaaata cgcaaaatac gccgaaaaga agcggcggta
     2221 aaatatcata tactctgcag gcgatcggcc tgattcccct gctggcgcta gggatcgtca
     2281 tgttattttt cacttcccaa tggttcacaa aaactatgta tcaggaagtg gaacgtgaat
     2341 tgtatgatgc cacaaaaagt gccactacac ttctgaacgc tgcctatccc ggcgattacc
     2401 ggctggaagg tgatgttgcc taccttctgt ataagggcga tgtagatatc acccgtgaat
     2461 attctctatt tgatcaattc aaagaggata ccggactgga catcaccctg ttctaccagg
     2521 atacccgcat tctgacgact ctgtataacg cccagggtga ccgcattgtc ggctccggtg
     2581 ctccggatgt ggtgatccgt gatgtcctga ataccggtga agatcatttt tatatcaaca
     2641 ctctgatcaa tggaaagact tatttttcct attatagtcc tgtccgcaat caggacggca
     2701 gcatagttgg catgcttttt ataggaaaat ccagtgccgc cgtatctcaa tccattcaac
     2761 gttatgtcta tccgctgaca tggctgatca tcggttttgt ggctctcgtg gctgtctgca
     2821 tctattttta caccagaaga ttcgtggctg tattactcca tattcacagc ttcctgtcgg
     2881 aggtagcctc cgggaatctg aacgccacac tggatcattc cgtaacaaaa cgcagtgatg
     2941 aattaggtga tatcggtcgc tgtgccctgt ccatgcagcg ctctcttcgt accatgatcg
     3001 agcaggatgc cctgaccgag ctgtacaacc gacgcagcgg ggaaaaaaag ctgcgccaga
     3061 tcttcgagga agcccaatcc tcccgccatt cttttgcgct cgccatcgga gacattgact
     3121 ttttcaaaaa ggttaacgat acctacggtc atgaatgcgg ggatgccgtg ctgaaaaatg
     3181 tttctgccct gctaaagcag catatgtggc gccgtggctt tgccgcgcgt tggggtggtg
     3241 aggaatttct actggtattt gaaaatgtag atcttgcaga agcacggaaa cagctggagc
     3301 ttctcatgga caaaatccac gaactggata cgctctatga aggtcaacac gtgaagataa
     3361 atatgacctt cggactggtc tgtgagtctg acaaagatgt tcatacatta ttaaaagaag
     3421 ctgatgagaa attatatatc ggtaagacaa acggtcgtaa ccaggtggta tcttaacccg
     3481 gttacgaccg tttcttattt gtttctgatc ttgttatctt taaccttgca agcattccgg
     3541 attcaacagg aaatcataaa tattgattgt cagaatacca tattcatcat aatatggttg
     3601 cacaatatct tttgtaatga caattttctt aaaagagtca tcaatcttcc ggaacggtcg
     3661 gatttcctgc gtccgctttg cttcatccgg aagagaatat gccgattgaa tgtaatatct
     3721 ggcagaacca agattgcaga caaaatccac ttccagctgc ctgcggttta cctttccttc
     3781 caaatctttt tctgcgatag ggacaacacc tacatccaca ctatatcccc gcatccggag
     3841 ttcgttatag attacatttt ccatggaatg tgtctgctcc aactgccgga aattaattct
     3901 ggcattccga agccccaaat cagaaaaata atatttctta ggagtctcta tatatgactt
     3961 tcccttaata tcataacgct gtgctgactc tatcaaaaaa gaatcttcaa agtaatccaa
     4021 gtatttcttt atggttgcag aagtaatttt agatttcttc acggttttaa aagtgttctt
     4081 tagtttttcc ggattggtca acgaaccgat cgcagaggaa agaatattta gcagatcttc
     4141 caattcccca atattcttta tacgattccg tttcgtaata tcacggatat aaatttcgtt
     4201 aaataaattc tgcagcgctg ttgccttatc cccggcacca tcccgcagaa ccactaatgg
     4261 aattccaccg taaagcatat attcagataa cccggtatat ttatctccgt tatgaacaga
     4321 cataaattca gcaaagctga gagggtacat atgaatttca tcacccctgc ctgcaaattc
     4381 cgtagcaata tcctttgaca acagttttgc gttacttccc gtcacaaaca catcaatatt
     4441 ctctttacga aggtatccat tcaaaacagc ctcaaaacaa tccagcatct gaatttcatc
     4501 caacagcaga taatatattc cctctcctgt agttttcgac cgaatatatg ccataaattt
     4561 ctccggatcc acaccccgct tttccttttc gatctgaata agtctttcac cgatcaggta
     4621 aagatcatcc gcagaatcaa aggcaaaccg tatgatatgg tctgcttcca caccggaatc
     4681 taacaaatga tgatagaaaa tgttattcag cagataagac ttaccacatc ttcgaatacc
     4741 ggtaatcacc ttgattaatc cattgctctt tctttttatt aacttttcca aatagaaatc
     4801 tctgcgaata tccatatatt ttctccctgg aatagatttt tgcaactggt tgcacttttc
     4861 tattctatta tatggataat ttaactgaaa atcaatatac ttgcttcatt tttggaaaag
     4921 atttttgcaa ctggttgcac ttttctattc caaattgtaa ataaacaaag taattattta
     4981 ttgttccatc tgtctgccca ggaagcccgc cgcagacaga gccagtttct gctccacttc
     5041 tcccattttg ctcttcccgt cattttccaa caggatcaca cttccgatga tgtccccttc
     5101 gcagatgatc ggcgtgatca gctgccaggc atagtccacg gcaatgtctt ccgcaatgtt
     5161 gacatagttg cggtctcctt ttgcggcaat cacagtctct ctattattga ccttctcttc
     5221 cagttgacgg ctcacggatt tatttaacag ctgcttacag cctcctgaga cggcgatgaa
     5281 ctggtctctg tccgcgatca gtgtggcatg tcccgacacc tgtgccaggc tctccgcata
     5341 ctgtttagca aatgtggtca tctctccgat aggggaatat tttttcagga tgacctgccc
     5401 ctctctgtcg gtgaagatct ccagcggatc actctctctg atgtgtaacg tgcggcggat
     5461 ctccttcggg atcacgatgc gccccaggtc atctattctt cttacaattc cagttgcttt
     5521 catcattttt tcctccatga catattgcgt aagataaatc atcatctcgc attactaatc
     5581 attaacgttg cagttagtat gcgaaatgca ggaaaaaata tactttaaat tgccagatgc
     5641 accgtcattc agcagtgcca ttgctggctt atatttatag cagttctgta ttttccctca
     5701 gaattattgt tttatattaa aattaaaatg catccattca atttttactc ttgcatgatc
     5761 gaatttttcc attctcaatc agactgtttc gtactgtatt ttccttattt ctatgttaca
     5821 cttattctgt ttacgacaat tttttatggg agagtttaaa ctatgaatca caaaaataat
     5881 aacagaagtt ccttttccgg caagatcggc ttcgtcctgt cggctgccgg agcatccgta
     5941 ggcttaggca atatctggcg tttcccgtat ctggcagcca aatacggcgg cggtatcttc
     6001 ctgttgatct atattatcct ggcgctgacc ttcggttata ccatgatcgt ggcggagact
     6061 tcactgggac gcatgacagg taaaagcccc gtgggtgcct ttcatacctt cgggaagtcc
     6121 aaatggctgt ccttcggtgg atggatcaac gctatcattc ctattctgat cgttccttat
     6181 tattccgtca tcggtggatg ggtgatcaag tatctggtgg aatatgccaa gggtcacgga
     6241 catgatctgg cagcggacgg ttatttttcc acgtttattt ccaatggtgt ctctacagag
     6301 ctgtgcttta tcgtattcac tctgtgtact ctgggcatca tctacgccgg tgtccgcaac
     6361 ggtatcgagc gtgtatcccg cattatgatg ccgatcctgg tagtgctctc tgtggtcatc
     6421 acagtctatt ccgttaccag accgggagcc ctgagcggtg tgaagtattt cctgatcccg
     6481 aatcccgcga atttctcctg gatgacagtc gtagctgcca tgggacagat gttctactct
     6541 ctgtccattg ccatgggtat cctcatcacc ttcggctctt atatgaaaaa agatgtctcc
     6601 attgaaggtt ccacgaaaaa tgtggagatc tttgataccg ccatcgccat catggcaggt
     6661 ctgatgatca ttcccgccgt gttcgcattc tccggtggag atcccgatac cttacaggca
     6721 ggtccttccc tgatgttcat cacacttccc aaggtcttcg acagcatggg catgggcacc
     6781 ttcgtaggta tcctgttctt cgtactggta ctattcgcag ccatgaccag ctccattgca
     6841 cttaccgaaa gtgctgtgtc tactttcgag gatgagcttg gctggggtcg taaaaaatcc
     6901 accttgatca tgggtgtgat catgctggca ctgggaacat tatcctcact gggttacgga
     6961 ccgcttggtg ctgtgaagat catcggcatg cagttccttg atttctttga tttcctgacc
     7021 aactccgtga tgatgcctat cgctgccatc accatctgtc tgttggtgtc ccgtgtcatc
     7081 ggagtaaagg ctgtggaaga agaagtcatg cacggagaag ccgctttccg ccgcaaaaag
     7141 gtattcaact tcatgatcaa gtatctctgc ccggtatttg ctgcgattat tttattaagc
     7201 tctgtggcaa atgcatttgg acttatttct atgtaagatg ccagggtcat aaacaagaac
     7261 cccacgctgt gctcgcacaa acgtggggtt cttgtttcat gacccgctcc gatattcgca
     7321 taaaagggaa gcgtcagctt cacgatatgc gaataccggg caggattgtg agagtgcgca
     7381 gcacggagca atccgtcagg catcttacat cttactatat gttaatgatc cgtcctgttt
     7441 ccgcatttac tctatgtgaa attgagatta ccgttcttcc ttccgaggca cgcttcagtg
     7501 cctgcagcac ctcttcctcg gtctgggcat ccagatttgc cgtaatctca tccaacagaa
     7561 ggagcttcgg cttcgccacc gcagctctgg caatagataa cagctgccac tgtccctggg
     7621 agaagatctc ctgggtacag ggagtgtcat agcctttttc caattgttct atcgtcgcat
     7681 ccagtccagc aattcttgca gccccccgta cctcttccat ggtaaaagaa tcgtcataca
     7741 gcgtgatctg atccttcacg gtaccgggta ctctatgaaa agtctgctcc acatagccgt
     7801 acaggctgcg cttttcatgt tccggaatct gaaaagcatc tcttccctga atcagcactt
     7861 taccctctcc cggctgatag agtcccaaca acagtttgaa gaccgtactc ttccctgctc
     7921 cggttcttcc ggacagtgtc acctgttctc cgggatatac ttgaaaattt aaagcctgca
     7981 aaatcttacg gtcatcatcc tcatagctaa aggtcacatt ttgaagttcc acaaaaggtt
     8041 tttccggtct attctgtggt tctgccggtt tttccccgga atttctctcc ggcaattcct
     8101 ccagcgcata gaattcattg atccggtgca cgcccgcaat ggcggactgt atggtctgga
     8161 tctccattcc cagactctcc acaggggtaa aaatctgtga gatatagtta attaccgcta
     8221 ccgctgtacc tgcagacata ccgaacaatg tcagcacctt tgcattaccg gaggcagaaa
     8281 acagcatcac aaccgccacc acgatagcat tcaggatcag gatcaccggg gaataaatgg
     8341 catcataaaa attcgtgcga tctaccgcgc ggtagctctc cgcaatatat tcatcatagc
     8401 gttgctccat gtagcgctct tttcccagcg tatgaatggt acggatattg tgcagcgtct
     8461 ccggcacatg tccggatgct ctgcccacgg cgcaacggtt ctcgatctgg gctttcagca
     8521 tatttttctg caccgtccgg gtgaaccaat acagaaatgg aagcagaacc aaaagtacga
     8581 tcgccagtcc actgtttttt aaccagatca ccaccaggat acttataatc ttacaggcat
     8641 cggcgaacat actgatgatt ccggaggtga acaggttctc caccgtatcc acatctccca
     8701 cgaaccggga tacgatcgta ccgggctcct gtttcgtcag ttcccctgcc gtcaggttcg
     8761 tgtatttctc catcatgctg ctccgcagtg catgagtgat cttctggccg aagaccgtca
     8821 gcagaccttc ccgaaggctt tcggtcagac cggtgagcac cgtaaaagca aaatatagta
     8881 tcaccaatgc aaccggaact gtgtttcctg cggtgatcgt atccacaata cttccgagga
     8941 tccagggcgg cagaagcgcg gttacgatgg cgcctgcaac tgccaacagg attcccagtg
     9001 acagccctgc atagcctgtc acagtctgcc ggatcacgct gcggacattt ttcacattct
     9061 gcctcttatt tttctgcatc tgtttatttt tctgcatgat ccggacctcc ctcctgcgta
     9121 ctgtgtgcat cagcgccatc cgcctgtgct tcatacagtc tcgcatactc cgggaacttt
     9181 tcaaggatct gttcgtgggt tcccgcagtc acttttccag cctccatcca tagcacctga
     9241 tccatttcgg ggaacagata gagtctgtgg gacagcagga tcacgatact gtccgctgta
     9301 aacttccgca gattggcgaa gacctcttcc tccgtctcac gatccagtgc ggaaaaagga
     9361 tcgtccagga tcaggaccgg acgcttatgg cacagggttc tcgccaatgc caaccgctgt
     9421 gcctgtccac cggagagccg gacgccgcca tttcccacaa tggtgtccat accctcttcc
     9481 atctctgcca cttcaccgtc gaaacagact gccttcagat acttctccgg atcgtcatca
     9541 tcgcccagca gtatattgtt acggacactg tcattgaaca gctccggatc atgtcccaag
     9601 tatcctatca tgccggtgcg ctccgctttg gtcatttcgg ataattcttt gccgtggaaa
     9661 cgtatccttc cttcgtaagg gtattcacag aggaaggtct tacccaatgt ggacttacca
     9721 cacgccactg ctccggtgac accgatgatc tgtcccggga aagcggacag attgaagtcc
     9781 tcatatatct tctctctgcc gggataaaca aagcttacat gagatacttc cagcattccc
     9841 ggcttctgta ccaagctgtc gtcctcataa gtgtcttcct tcatcagtgg tttgatacgc
     9901 ttccaggaga cctgggcttt atgtaccgca ttgaatagct tcgctgcatg agaagatttc
     9961 acggctaact tcaaaaagca ggataaaaac gtggtaaacg ctgccacatc ccatgatttc
    10021 cagccggtgc ccagcacatt tctactgccg aaatacagga taaacagtac gccgatcatg
    10081 gagatgatcc ggtacagcgg cggaaatgcg gtactccaga tattggcctt cacagcagat
    10141 ttttcataag cggcaagatt ctcttcatag gcctgctccc gctctttctc tctgccatag
    10201 acacggtaag taacagcgtt agttgctctg tccagtgttg cgtcgcttaa atctccggac
    10261 tgctccttat aagctgctcc ggtgcgttgc acgttacgct tcatcttctc cgccagcaca
    10321 tatgacgccg gtaaaaacaa catacctaag attgccagcc gccagtcata gaataacaac
    10381 attcccacat atgccgccag tgccacaccg gtatcgaaga tctctgtggt aaatttcctc
    10441 atcccctccg cgcagtcatc cacatccagg attgccttgg tcatgacatt tccagcaccc
    10501 tcttcttcca gttcggcacg gcttctgtgc accagactgt ggtaaagggt ctctttcatg
    10561 tcacgattca cattattagc aaatcgtctg acataaaaac gtttaatata acgggagatc
    10621 tgcacgatgg caatagacat cacatacgca gtcaccagca ccagcatatc ccggtatcct
    10681 gctttcccac ccagaatgtc caccagacat cccgtcatcc gcccctcgaa ccacggtcct
    10741 gccagaagtc cgaggttata gatcagtccg gagaacgtga ctgccagcag cacaggccac
    10801 tgggttttga aataatacag gatctgatcc tttttcgctg cctcagatat ctgtttcatc
    10861 ctctcctgct ccttcttcca cacatttttc cgaattgaca aatcctgtcc ttctcacgtc
    10921 acccatcaat tcggcttctt tcctcagcac tacataaaaa gatgtactat cctctgattt
    10981 agatgatacc agggtcaaaa ggtctaagcc cacatgagct tgctcataag tgggtgaaag
    11041 accttatgac ccgccccgat atgagcataa aagtgggatg ataatcccac gatatgcgaa
    11101 tattgggtag ggtgctatgt gccagtggca catgtctagc acggaccgaa gcggagcgga
    11161 gacgtgggag tgcgtagcac gaagcaatcc gtaggtatca aagtcggggg cgtttgaccc
    11221 cgacatcatt tagataatag tacatccttt tcatctgcca atgtattggt attggctctc
    11281 ttgcgttcat tatctcaggc cttcagaatc tccgacagat tcctctggat cccggctgcc
    11341 acatccggtt tattcatgga atagacatgg atatgtttca cgccgttggc caacaggtcg
    11401 atgatctgat cggtggcata ggcaatgcct gcctgtttca tggcttccgg attgtctcca
    11461 aagcggtcta cgatattttt gaatcgttcc ggcacattac aaccggacag ttttacggca
    11521 ttcttcacct gcacgccacg ggtgatgggc atgatgccgg gaacgatggg cacggtaatc
    11581 cctgcttccc gcacacggta catgaagttg aagaagatat tgttgtcgaa gaacatctgg
    11641 gtcgtcaggt actcgcatc
//
LOCUS       DAWEJW010000088        11655 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1940_length_11655_cov_55.550000, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000088 DAWEJW010000000
VERSION     DAWEJW010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11655)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1940_length_11655_cov_55.550000"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8034..8133
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3333 a         2370 c         3124 g         2728 t
ORIGIN      
        1 taactttcta tttatacaat cacaatcggc gtccgatatt cattcaccat ttccgtctgg
       61 ttctttaagg ccagattccc cagatagatc tgattgcgga ggatatccag atccagcatc
      121 cgcagagatt cgggagaaag cgtctgtctg gcgtcagcca ggctggtgac gagaggaatg
      181 gaactgttct ttttgatctc ggtaagcagt ggcgtcgcat tcttgcggaa acccaacact
      241 ctggcgtaag agatatagtc ttccgacttg caggtctcga attcttcctt ggtcatattc
      301 agcaaaatat gaagcagaca tctgctgatg cgggtgtagg tcatatcctt actcttgagc
      361 agatcacaga aagaagtgta acctgcgaag gaaggcaggg tattgcggat ccggtcggac
      421 agatccgaag aaatatcctg atacttgtcg tagcccacag agtattccgt cagcagcttg
      481 tattgcagtg ctgcggaaaa atcatcggca aacagaggct ttttgtgaga cagagaatcc
      541 gtcatcaatg cataggcact ttccggcatc tgggcagcca gataggatag atcatgcccg
      601 gcggccaccg actggcggat agcaatggcg gaacactgat tggtgccgag acgtttgtcg
      661 tgataatccg caccgactct ggtggtagtg aacggtgaga tcttgctgtt tctggacatc
      721 agagctttga gatattcaat tcccaatata ttattaggag aggaaagaac gtccttatca
      781 tcgcttaagg aaggcgcgta ttgcaataac gcccaggtcc tggcgatggg gaaggacatt
      841 cccttacgca tattgcagcg caaggattcc acgtaaggtt ccggttccgt atagaaaatc
      901 tcagcgatcc gtgacaagac ttcaatgttg ccgcactcac tgccaaagca cagatttgtg
      961 gtcactccca gtttgtcgaa caaggcaacc gagcccttgg caaaaaattc cgcactggag
     1021 gcagcatagc gggtgggcag ttccagcacc agatctgcac cgttctccag agccattttt
     1081 gtacgggtga acttatctaa gagggcagga gcaccccgct gcatgaaatt accgctcatg
     1141 acaatgatcg tataatcggc atcattggcc tctttggcat gctgtaactg gtaagcatgg
     1201 ccattatgga agggattata ctctgcgaca ataccattga ccttcataat gtggctcctt
     1261 tcgtgaaaag aaatatacgt tatagcgaac acaaaccctg acagggaatg tgaaaaaaat
     1321 aacatacact gaaagcgttt acatatgcat ggttatcagc ttattttatt ctgaaaaaat
     1381 agtggttgta tctgaaaata tacaattttg cgaacattat gcccttgtgt actccagatg
     1441 tatttagtat aatataaata catcggtttg ttaagataaa accaaaactt tacagaagtt
     1501 atttgggagg aagcgggtat cccccgccgg aaaggagaat ggatatgtca tacattgaca
     1561 tgattaaaga gagagcaaga ctcgacaaaa agactattgt attaccggaa tctaatgata
     1621 agagaacatt actggctgct gcccgtatcg tggaagaggg catcgcagac atcattatga
     1681 tcggtgatga agagaagatc atggatggtg caggctggct ggaagttgac ctgtccaaag
     1741 ttaaggtagt taaccctaaa acaactccca agttggatga ctatgtgaat ttattatatg
     1801 aaaccagaaa agcaaaggga atgacccccg agaaggcaag agagatcctg ctgaatgatt
     1861 acctgacctt cggtatcgta atggttaagg ctaatgatgc agacggtatg gtagcaggtg
     1921 catgccactc taccgcagat actttaagac ctgcactgca gatcctgaag actgcacccg
     1981 gcgttaaact ggtatccgca ttcttcgtta tggataccgt attcaaggat cagggtgaga
     2041 acggaacctt cctgttcgct gactgtggtc tgaaccagga tcctactccc gaagaactgg
     2101 ctgctatcgc agatacttct tccagaagct tcaagtctct gatcggacct aagcccgtga
     2161 tcgccatgct gagccactcc accaagggaa gtgcaaagca cgcactggtt gacaaagtag
     2221 tagaagctac ccgtatcgca catgaggagt atcctcatct gactctggac ggcgaactgc
     2281 agttggatgc agctctggtt cccagcgttg ccaagtccaa ggctcccgga agccctgtaa
     2341 acggacatgc caatgtcctg atcttcccga acctggatgc tggtaacatc ggttacaagc
     2401 tggtacagag actgggtgga gcagaagctt acggccctat gttacagggt atcgcaaagc
     2461 ctgttaacga tctgtccaga ggatgttcct ggcaggatat cgtcggtgtc gtagcactga
     2521 ccgctgtaca ggcacagtta gtataatcac tggtaacgaa cataaacgtg tgcgggatat
     2581 ctgtcccgca ccgtattcat gtattgcccg cacgggtaac atgaccccat agagtaacag
     2641 atttgaaaga aaggaattca tcatgaatat tttagttatt aactgcggaa gctcttccct
     2701 gaagttccag ctcattgatt ccgagaccga gaaatgcatc gcaaagggtc tttgtgagag
     2761 aatcggtatc gaaggaagcc agattaccta tacccccgat ggtggtgaga aagagcagac
     2821 tgtaactccc atgccggatc ataccgaagc tatccgtctg gtactggaag ctctgaccaa
     2881 tgaaaagaca ggtgtggtaa agagtctgga tgagatcggt gccgtaggac atcgtatcgt
     2941 acacggcggc gagaagttcg cagcatccac cattatcacc gatgaagtta tgaaagccat
     3001 cgaggaatgc aacgacctgg ctcctttaca taaccctgct aacctgatcg gtattaatgc
     3061 atgcaagaag ctgatgccca ccactcccat ggtagctgta tttgacaccg ctttccatca
     3121 gaccatgcct gaggaagctt atatgtacgg tctgccttac gagtattatg agaaatacaa
     3181 gatcagacgt tacggcttcc acggaaccag ccactcttat gtatccaaga aggctgctga
     3241 ggttctgggc aaaaagtacg aagatctgaa gatcatcgtt tgccacctgg gcaatggtgc
     3301 cagcgtatcc gctgttaaga acggcaagtg tgtagacacc tccatgggtc tgacacctct
     3361 ggaaggtctg atcatgggaa cccgttccgg tgatatcgat cctgccatca tggagttcat
     3421 cgctcacaaa gagggcaaga acatcgacga gatcatgacc gttctgaaca agaagtccgg
     3481 tgtatatggt ctgtccaaca atctgtcttc cgatttccgt gatctggaag caggttacaa
     3541 caacggcgat gcacactgca tccgtaccat gaacacttac tgctaccgtg tggcaaaata
     3601 tatcggctct tatgtagcag ccatgaacgg tgtagatgtg atctgcttta ccgcaggtat
     3661 cggcgagaac gcacctttag ttcgttccct ggtatgcgag catctaggct tcttgggagt
     3721 atccatcgat gaggaggcaa accacaagcg tggcgaggag atcgctattt ccactcctga
     3781 cagcaagacc actgttatgg taattcctac gaatgaggaa cttgctattg ccagagagac
     3841 tgtaagtttg gtaaaatagg ttcgacggaa ccttaagaat gagaacaccc atcaaccgga
     3901 ggatccgatt ggtgggtgtt ttacgtatgc aaatcgagca ggagaagtcc ccgtcgatgt
     3961 atggtaacga atcaacagag ggagaaaaat tatgaaaaaa aatgtgattg taggacagtc
     4021 cggaggcccc acagcggtga tcaatgccag tctggcaggt gtctacaaga cagcaaagga
     4081 tatgggagcg gataccatat acggtatgcg ctatggcatt cagggtttgt tggagaaaaa
     4141 gatcgtggat ctgggggaaa agatccgcaa cgacatggat gtggaacttt tgaagagaac
     4201 accggcttcc tttctgggat cctgccgcta taagctgccg gagatcagtg aagataaagc
     4261 tatttacgag aaaatatttg caattctgga agaactggag atcatggcat ttttctatat
     4321 cggcggaaat gactccatgg ataccatcaa aaagctttcc gattatgcag agacagtggg
     4381 caacccgatc cgttttatcg gcgtacccaa gaccattgat aacgacttag agggtacgga
     4441 tcatacgcct ggttacggaa gtgcggcaaa atatattgct accgttacca aggagctggt
     4501 acgtgacggc ctgatctatg agatgcagag tgtgactgtc atcgagatca tgggaagaaa
     4561 tgccggatgg ctcaccggag cagcagtact ggccaagagt gaagactgtg aaggcccgga
     4621 tctgatctac ctgccggaag tagcctttga tgtggatgat tttctgaaaa aggtcaagaa
     4681 actggttagc gagaaacagt ccattgtcat tgcagtgtcc gaaggaatca agacggcaga
     4741 cggaagatat gtctgcgaat tgtctgcacc ttccgacaag acggattctt tcggccatgt
     4801 acagttatcc ggtacgggca aatatttgtc tgacctgatc attgaaaagc tgggctgcaa
     4861 atccagagct attgaactgt ctactctgca gcgctgtgcc ggacatctga cctcccgcgt
     4921 ggatatcacc gaggcttatc aggtaggtgg aactgcggta aaggcagctt ttgaaaacaa
     4981 gaccggtcag atggtgatct taaagagagt atcccaggat ccctatattt gtactacgga
     5041 tctctatgat gtacacaaag tagccaatct ggagaaaaag gtgcctcgtt cctggatcaa
     5101 cgaagcaggc gattatgtga cggaagatat gaaaaattat atcgaacctt tgatccaggc
     5161 ggagctgact ccgatcatga tcacaggaca acccagacac attattattg acgatctgta
     5221 aaaaaaaatg gaataaaaca acaaatcaaa taacgataca aaggaggtgg aaagatgccg
     5281 aaacacagga agagaagaat catgctcagc atagtgagta ttgtgctttt tgtagtttta
     5341 tctgcgcagc tttccacctt ttctttttct gtgactgcgg ccggggaaca cggagaactg
     5401 gtggcggagg agtacggacg ataccgggag cgctttgaca ggatagaaaa gattggagat
     5461 ttggagaacc agggattccg cctgctggag gatcagatct ttgcgatgcc gttgcaaaaa
     5521 cttccggagg aagccgtaga tacgacagag gaacccgggg atgaagtctg gttctatgcg
     5581 gcactggata caaggtacca cagactggcg gtatttatcg cagatgcttc cgggcagatt
     5641 ctgtacaaga cggaccagct ggaagcaaat tactgctatc cgggagaaat gcggcagccg
     5701 gtgaaaaaac tggcttcggt ttcttttcag gatgtagaca atgacagaga tacggatatc
     5761 atcctgatcg tccagtgcca taatgacagg ggggattatc aggaagaatc ctacaaagta
     5821 ggagatgttc tgttccagga cgatggaaac ttttaccgtg attatcggat ctcagacaag
     5881 atcaaccgct ttgatatgaa caagaaccct gcctgtattt taaattttgt aagagacggg
     5941 cgttctacgg aattcctcta tacggcggag acactggcag atcttctgaa ccacaatttc
     6001 aatgtcatcg aggaacagtc ttatacccgt aattttgaaa agctgggaaa attaaaggtg
     6061 gttccgggca cctaccggat ggcggaatat gacgtgttca tgatctatct gatcgatgaa
     6121 cagggaaata ttgtgtggag ttttcaaccc atggaggatt acgacaatct ctatgcgctg
     6181 aagggaatcc agggaaagga cctggacggt gacggcctga aggatctggt ggtgtttgca
     6241 aaatacagct atgaaggaga aaacggggaa ttgctggtgg acaccgtgtg caccatttat
     6301 taccaacgga cggccggatt tgagaaagat actgatttta cagaaaatta tgaatgtacg
     6361 gaggaggata ctctggaggc actggtgacg aagatccgtg cctattgggg gtggcagaca
     6421 taatgataaa aatattgatc gtggatgatg aaaagccaat ctgtgacctg atcgacctga
     6481 atctgtcggc agcaggatat ttttgcaaaa gtgtacagga tggcatgaaa gccattgaac
     6541 tgattgaaaa ggaaaccttt gatctgatct tactggatat tatgcttccg ggagtggacg
     6601 gttttgatat tatggaatat atcagacctt taaagatccc ggtgatcttc attaccgcca
     6661 agggagatgt aaaggaccgg gtaaaaggct tgcgtctggg ggcggaggat tatctggtga
     6721 agccctttga tgtggtggaa ctgctggcaa gggtagaagt ggtactgcgc agattccaca
     6781 aacggggagc gcagctgtcc gcaggagatg tagtggtgga tatggaagcc agaatggtga
     6841 tgaaagcggg cgtacctgtg gtacttacca acaaggaata cggacttctg gtgttattta
     6901 ttcagaataa aaatattgcc ttattcaagg aaagcctgta cgaaaaggta tggggagaag
     6961 aatatgacgg ggacagcagg actctggagc tgcatgtgca gcgcctgcgc aaaaaaatgg
     7021 gatgggagaa tcagctggta gcggtatata aggtaggata ccggctggag attgcctgag
     7081 aacgaggaat tcttgtagga aagtggacaa gaggagaaaa gaatgaaatt ttcctggaaa
     7141 attctgctgt gcaccatgct gatcatggca gcggcatgcg gagcaagcgg ttattttttt
     7201 gtaaattatg tgtttcagac ctccatggag cgtgagacca gacaggcact tgatgaaagt
     7261 agtattttga ggttcgcttt tgagacggca gctttaaata ttccgtccaa atatgatgta
     7321 cttccggacg gaacggtgga acagatcgga gtatacctgg agaacagtgg acagcacggc
     7381 aacagattgc tgcggatcag cgatgagaac ggtaaggtgc tgtatgtcag tgaagggttt
     7441 accggggata cttctctgtg ggaagaacgc ggagaaaata cgagagtgta ccgggtagtg
     7501 cagtcggggg attcctatta tatccagaca cagacaagga tcaatgtgtc cgaccgtctg
     7561 ttgacgttag agaccatgaa aaatgtaact gcggtatata cggagaggac ggaaggattt
     7621 cggatgtatc gtcaggtgac ggtgatcgta ctgttgtgca gtggcgctgt gatgacgctg
     7681 atcgcctgct ggctgacccg accgatccgg cttctgacgc aggctaccgg caaaatggcg
     7741 gagggagaat acagctatcg cgcggagcag atcagtaatg atgaaatggg acagctgacc
     7801 gcggatttca accatatggc agaagcactg gagcagaata ttcagaattt ggaaaatgaa
     7861 gtcagagcca gagaagattt tattgccgca ttttcccatg aactgaagac accattgact
     7921 gccattatcg gttatgcgga tatgctgcgt tcccggaaat tggatgatga gaggcatttt
     7981 ttgtgtgcca attatattta tacagaggga aaacgcctgg agactatggc actnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgcatca cagcagcctg aagatcgaga
     8161 gtacacaggg ttttggaagc tgcatgagct ttctgattcc ggatgagggg gtgaagtcgg
     8221 atgaatagat ggaagagctg ttctttgggc tttgcggtat cagttgtggt cattcttgcg
     8281 gctctgtggg gaccggaatg gatcgcggcc aaaagtgatg agagactctt aaacagtatt
     8341 accacagagg cggtggaagg tgcggaagga taccgttacc gcatgagcag caatcagaaa
     8401 ctatatcttt tggggagatg tctgagtagt cagaccctgc cggagagtga actgcgtttt
     8461 ttgaccagag tggacagtga ggcaggaaac tacggagaaa tgacgggtac ctatgcgttt
     8521 gtagagaatc gtcagcaacc gggagaggga cagattcagg aggaagcggt atatgaggca
     8581 tgtaacagag agattcagat cctgaaggag cagggaattc ttcccggcga agtaaaggaa
     8641 gtttcggaag actcttacga agcggtgatc tgttctgcta tcgatgtttt ggagccgaga
     8701 aataatctct ccgtatggaa aatcagcctg tctacggatg taaggaatgc ggataagagt
     8761 aaccgttttc tggatattta tctggatgcg gataccggca aaatttatga attctatgtc
     8821 cggaccggac tgcaatggga agacatcaat acggatgcca tgattggccg gtatgcggaa
     8881 tatctggaac tgaccggtct tgaaaaatat gaggatcaaa atccgctgtt ggagacgacg
     8941 ccgtattttg ccaagtatac tttcccgggg gaggaggaag acagtaccac ggtgaccatt
     9001 ggttactatg aaggaattcg tgagcttttc ctcaaagtag gaagatagaa aaataaaata
     9061 cgcaaagatt tttttgaaaa aagattaaaa tcactacttg acgttaccac actaaagcag
     9121 taatataagt aaaaattaaa tacagcggta agaaaccatt gatgtggaga taaaaacagt
     9181 tcgtgcactt acagagagac cggaggctga gaaaggttgg aacgcaggtt gttaaatgga
     9241 ccacggaggg cgcagtcaaa gaggacacac agaattttaa gacctcaagt attctgcgac
     9301 gtgagggcat acgtcagttg ccggggatat gttggtatcc tgtaagagca caagaaattg
     9361 tgaagcaagg tggcaccgcg ggaaatgtgt atgacccgtc cttgacagaa gaagattctg
     9421 tcatgggcgg tttttttgta accttttcag agcctatggc gagatagcct caatgagtaa
     9481 agggaagcct caaagaggcg attttacgaa tggaggcgct gaaaaggtgt gagcagaaat
     9541 aattttaagg aggagtaccg gaatgaagat cacccccaca ttggaagaat gtaaaatcat
     9601 cgcagcggaa ggcagctacg gcgtaattcc catcagcaca gagctgtatg cggatatgac
     9661 cacacctatc gaagtgttgc gcatcttaaa aaaagtcagt gggcatgtct atctgctgga
     9721 aagtgcggag gcggataaac gatggggacg atattccttc ttaggctacg atcctctgtt
     9781 ggagatcacc tgttataacg gtatgactac aataaaatcg gaactgacca gccggacgga
     9841 ttccggagat gtcagaggga tcatccgcaa cgtcatggaa gagaataaaa gtcccaagct
     9901 tccgggactg ccttccttta ccggaggtct ggtaggatat ttttcctatg actatatcaa
     9961 atacagcgaa ccgaccctgc ggctggatgc gaaggacgag gaaggcttcc aggatgtgaa
    10021 cctgatgctg ttccacaaag tcatcgcttt tgacaattac agacagaagc tgatcctgat
    10081 cctgaatatc aagaccgatg atctggagat caattatcat agagcggagc gggaactgaa
    10141 caacatgaag gagctcttgc tgaatggaga aaaggcagag catattccct gtaagctccg
    10201 gacagatttt caaccactgt tcaacgaagc agcctattgc aacatggtgg agaaagccaa
    10261 gcattacatc agagagggag atatcttcca ggtggtatta tccaatcgcc tggaggcgga
    10321 gatggaagga agcctcttag atgcttaccg ggtactccgc acaacaaatc cttctccgta
    10381 catgttctat ttctccggca gcgatggcga gatcgtggga gcgagcccgg agacattggt
    10441 gaaactggaa gatggtgtat tacatacctt ccccctggca ggaacgagac ccagaggagc
    10501 taccgaggaa gcggacaaag cactggaagc agaacttctg gcagacgaaa aggaacgttc
    10561 cgaacacaat atgctggtgg atctgggacg aaacgatatc ggcaagatca gccggatcgg
    10621 caccgtggaa gtggaaaagt atatgtccat cgagcgttac tctcacgtga tgcacatcgg
    10681 atctacggta agaggtgtga tcagagagga tgcggatgct ttggatgcgg tggatgccat
    10741 cctccccgca ggaactctgt cgggagcacc gaaacttcgg gcctgtgaga tcatcaacga
    10801 actggaagac aataagagag gcatctatgg aggagccatt ggctatatct cctttaccgg
    10861 aaacctggat acctgtatcg ctataagact tgctttttcc aagaacggaa aagtattcgt
    10921 aagatccggt gccggtattg tggcggacag tgtaccggag aaggaataca gggaatgcat
    10981 tcagaaggca gcagctgtaa gacaggcact acagaaagca caggagggaa tggaagcatg
    11041 atattactga tcgacaatta tgacagtttt tcttataacg tatatcagct ggtaggaagc
    11101 gtaaatccgg atattcgggt gatccgcaat gatgaatgca gtgtggaaga gatccgggtc
    11161 atgaatcctt cccatattat cttatcgccg gggcccggaa ggccggacaa agccggtgtc
    11221 tgtgaagaag tgattcgggc actgggcgga aaaatcccca ttcttggtat ctgcctggga
    11281 catcaggcca tctgcgaggt ggcgggagcc actgtgactt atgcatccca tctgatgcac
    11341 ggtaaacagt ccctggcgac tttggataca gacagtgtat tgttccgggg tatgaaaaaa
    11401 gtgatcactg tggcaagata tcattctctg gtggcagatc cacagacgat tcccgcagaa
    11461 ttgaaagtta ccgcggtcac cgaagacgga gaggtcatgg cggtagaaca gacagaaaag
    11521 cagatctacg gagtacagtt ccatccggaa tcggtgctga caccggatgg tcgacagatc
    11581 attgttaatt ttttacagac acagcctgca aataaaaagt caaggctaaa atgaaagaac
    11641 tttgaaaatt ttata
//
LOCUS       DAWEJW010000089        11488 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_1971_length_11488_cov_38.844573, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000089 DAWEJW010000000
VERSION     DAWEJW010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11488)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1971_length_11488_cov_38.844573"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3034 a         2636 c         2976 g         2842 t
ORIGIN      
        1 aaatggaaaa ttcctatcgt ttttttgaga ataaagattg taaatatttc ccctgtcaca
       61 agggacttgc agatttcaac tgcctgttct gctactgccc catgtaccgc ctggagcact
      121 gcccggggga tccccggtat tttgagaaaa acggcagacg gatcaaggat tgctcgggct
      181 gcaccttccc acacgatccg gagcactatg atacgattat gcagatcctg cgggagaatt
      241 agacactgtc cgacagctgc agcgaaaatg gtagcaggtc cggcgggaga ttattccgcc
      301 ggtatccatc cgtcctcgcc gacgaggacg ttttctttgg agaaatacgt ctccacctcc
      361 ggatttacaa ggcatctgga gagagggctg cgtccggaac agtccgcacc gggaccgaag
      421 ttcccatatt caatataatc tgacaacact tctgcacgaa cttttcccca gttctgccag
      481 ccttcgggac agatatgatc ccccagctca caacagataa acgctgtctt cgcgcagtct
      541 ctccagggac gaccgagata gacggagtcc ggctcctcgc ctgtgatccg acaatgcgag
      601 aatataagac cgaattcctg tcctgcggga gtgctggcgg cggtgatata tgctttctcc
      661 tcgctgactc tggcgatgga atggatctca cagtgatcga agaatgcagt attcggtccg
      721 aagatatagt ccacatcgcc gcagacataa caatccttga aataattccg atagaactta
      781 tgcacgatcg gcacttccga agcctgaaag aagtccggca gcatcagttt cttcatatta
      841 caagtcatcg ggtctccatt gaagattgta tcctgccacc ctgtgaaacg acagttgtaa
      901 cagctcaacc ggtcggctgc tgcatagact gccagcgcct gcccagcgag atatccggga
      961 ccggcggtgc tctcgatcgt cagattccgc agcgtgatat cttttccggc gataaaaatt
     1021 accgccgtat taaacgtccc atactctccg gagccatccg gacggggctt ctttgcaccg
     1081 tcgccgtaac ggaagacggt catcagggga tcatctccca caatctcaat attttctttg
     1141 cggataaata acttctcttc atagatgccc ggtgccacct cgatccggat cgtctcgtca
     1201 tgacgatcgt cgatggacag gatcgcctcc tgtaacgtag agaacgcaga cgggtcattt
     1261 ttagatacgt gtatcgtagt cattttctta atattcctcc tcaatcaatc tctgatagat
     1321 cttaaaattt ctaaaacaat tgtaaatact gggtttccac aacgcaatta taaaaaaatg
     1381 aactactttc aaattcttgt aatctgttat tttttgagat tatttgaaat tttttggatt
     1441 gagaatctct ccggagacat gatatgtata atgcaacgga cggacacagc gccggaccac
     1501 agagaggagg aaatgcaatg cctggaaaac aaaaagggca ggtcgtaaac agacaacatg
     1561 ggaacaataa agtagcggaa cactttcgta aatattatca gttatggctg ctggcacttc
     1621 ccggaatcgc acttacattg atgtttgcct acatccccat gtcgggactg gtcatcatat
     1681 ttaaggatta caattttaag gacggaatct tcggaagccc ttgggtggga ttgaaaaact
     1741 ttgaattctt cttcgccaac ttcagtaacg catggagggc gacgaagaac acgatcatcc
     1801 tgaatctgtt ttataccgta ttcggtacgg tggcagcggt agggctcgcg atcatgttca
     1861 acgagattcg tcacaagaaa ttcctcaagg tcagccagtc gctgtcgatc atgccttact
     1921 ttatttcatg ggtagtggca ggtggtattt taagagcact gctcaattac gacggcggcg
     1981 cgatcaataa cttattggta agcattggct tcgaacgcct ggatttctat aacgatccga
     2041 agtactggag agtcatcctc accctgtgca atatatggaa atcagcagga tacaacggta
     2101 tcatctattt ttcgacgatc gcaggcttcg acacctcgct ctacgaatcg gcgcaggtag
     2161 acggcgcgaa cctctggcag cggatccgtt acattacaat ccctctgctg aagccgacga
     2221 ttatcatcct gttcctgctg tccatcggta agatcatgag cggcgacctg accatgatga
     2281 tgtccatcac aaatctgtct ccgatgctgt tagatacgac agatatcatc gatacttacg
     2341 tatatcgttc ggttatcggt gtgggcgact ttgtaatggg ttctgccgtg gggctgtacc
     2401 agtccatctt cggctttgtt ctggtactgt tctccaactg gctggtagga cgttttgaca
     2461 aggattatca attattctag gaggaagatc atggaaaaac agaaaaaaat aaaacgttgt
     2521 gcctccgaca gggtattgat cacaatattt tacatattag tagcgatttt cgcaattgta
     2581 tgtctctatc cgctgctgct cgcgatcgga acctctttcg cggatgaagc ctccgtaact
     2641 gtcaacggtt accgtgttat cccgaaaaag ttcagtctct cagcgtacca gatgatcctt
     2701 ggatccatgg gtaaaaatct gttgaatgcc tatacggtga ccattggcgt tacagtattc
     2761 ggtacacttc tctcggttct tgtatctgcg gcttttgcct atgtgctgac cgtgagagag
     2821 ttcaaaccga agggagcatt gaatatgttc ctctacatcc cgatgatttt ttccgcagga
     2881 cttctgccct ggtacatcat gtgtaccaaa tattaccatc tgacagacag cttcctcgca
     2941 ctggtgctgc cctgctgtat gaacgccttc aacgtgttcc tgctacgcaa tttcttcaag
     3001 tccattccgg aggagctggc agaagcggcg aagatcgacg gagcgaacta tctccgcatc
     3061 ttccgcgtga tctacttccc gctggcaaag gtaggtctcg tgacggtcgg actgttctac
     3121 gcactgagct actggaacga ttactatctg tcgctgatgt ttatcaataa acagaatatg
     3181 tatcccttac agtattatct gtacaatatg ctgtctaatg tacagttcat ggcaaatcag
     3241 gcgaatgcct ccctcggata caacatcaat attccgttgg agacgaccaa gatggcaacg
     3301 atctgtgtaa cggtcggacc gatcatacta ctctaccctt tcgcacagaa atacatcaca
     3361 aagggtgtga tcgtcggagc agtcaaggga taacacagga tcattaaaaa aggtttctaa
     3421 aaaatagcaa catagagtac aataaaacac aggaggttac ttatgaaaag aaactggagt
     3481 aaaaaagcac tttccctgct gatggcagga accatgatgg cgggactggc aggctgcggt
     3541 tccgatgcag cacagaatga cagcagcgcc gcggcatcca ccgcgcagag cagtgccgca
     3601 cagagcacgg atgcgagtac cagcgacaac ccgctgcgtc ccgatactct ggataccatc
     3661 acggttgtat tgttcggtga ggagtctccc cgtatgcagc agttaatgga agaaaaatat
     3721 cagcaggtat tcgttgatga gatcaacacc aaggtagaac tgatgtatct gccctggacc
     3781 gagaacggcg caggtggtaa ggttgacctg atgatcgctt ccggacagga attcgatgcc
     3841 tgcatcgtag atcctacttg ggctgccagc agctacagca agggttattt acaggatctc
     3901 tccgatgtga ttgacaaata tctgcccgac tggcacgaga atatggatgc tactgcattc
     3961 gacgcttacc gttatgacgg cggcgtttat gcgatcccca tcggtaacaa gccgaccgcc
     4021 ggtgtataca ataccgtatg tgtaagacag gatatcatgg atgccatcgg catggacaat
     4081 atcgcaacgt tagacgacct taccgcatat gctgaaaaag caaaagagca gtacggctta
     4141 tatgcgacct acgagctggc agatgcagct tacatcatcc gtggtaccag cgaccgcaat
     4201 ctgattactg tagacaagag caatctgtgg atcgaccagg ataccaagga attcgtaagc
     4261 ctcgtggatt ctccggagtt tgaggcagct gtaaagctgt acaataactg gtacaacgag
     4321 ggactgatcc caaaggatat tctgaccaat accgtaaccc tgcctttcca ggcgaacatg
     4381 agctctctga tgcgaggaac ctgcggcact accctgatcg agaacgagcc gggtctccag
     4441 actgtcgtac ccgaaggcaa gactgctgag tactatatca gccccgagaa gccgatctac
     4501 aaaaactctt acgagaatac cgcattccag gtacccgtta cctccgacaa ggctgaccgt
     4561 gtagcgatgt tcgtaaatct gttacagaag aacacagagc tggcaaacct gttcgcatac
     4621 ggcgtagagg gaaccgatta tgagctgatc gatggtaaag tatccaagat caataacgat
     4681 gagctgttct atgagtggat gatctacaac gtaaatattt ccaccccgac tactgcttat
     4741 accgatgagt ttatggaagt atataagaac tgggataacg gtgcaaagcc ctcagcaacc
     4801 ttcggcttca atattgatta ctccaacatc aagaccgaga aagcacagat tgacaacgta
     4861 tgggatgagc ttgcgaaacc tatgctggca ggtctgaagg actatgactc caatattgac
     4921 gaactgagat ctgcactgaa ggcagcaggc tgggatacct atgtagcaga gatccagaag
     4981 cagtacgacg aatttctggc taataaataa atgattttcg ggctgcctgt tcttgcaaca
     5041 ggcagctttt tttcttaaaa tagataataa agattatgag gagatttcgg gatgaacaat
     5101 aaatggcaga acgcagtccg gaacatggaa cagcggatgg aaagcctgcg gttaaatatg
     5161 cacggctatc agatccttta tcggggggag ttgatcggcg aacgttactg ggagcctttt
     5221 catagggaca gcctgcaccg gatgtattcg attacgaaga gcttcacctc gctggcgacg
     5281 ggactgctgg cagcggaggg caagattggc ttagacgacc ggatctgtac gtattttccg
     5341 gaaaagctgc cggagggtgg cgcacatcca tggtgcgaag aaatgacgat ccgggattta
     5401 cttacgatgc gtacctgtta tgaatccacg acctttaaac gctatgacgg tgattgggtg
     5461 gagtcctttt tccgggtgac accggatcat gtgccgggaa cggtgttcaa ttacgacaca
     5521 tcggcgtccc atgtgctggc ggcgctggtg gagaagctga ccggcatgga tctgttggac
     5581 tatctgcgtg aaaaagtgct gcgccgggtc ggattctctg aggaagccta tattatcaag
     5641 gatccctacg gtgtcagtca gggcggaagc ggtcttgtat gcagactggg cgaccttgcg
     5701 ggaatcgcca tgttatgctg ccgcggcggt attttggatg gtgagcagtt gatcccggca
     5761 gattttctga gggaagcaac cgcttgtcag gtgccgacag atctccagcc cacgtttgat
     5821 gagcagctgg gctacggcta tatgatgtgg aagacccgtt atgacggttt tgtattctat
     5881 ggaatgggtg gtcagctggc gctctgtttc cccgaatggg atcttgtatt ccttacgatg
     5941 gcagatacga tcgggaatcc agcgggactg cagatgatct atgactgctt ttatgatacg
     6001 gtatatcctg cggtgacagg agtgtcggaa tatacaaaca cacaaaaact gccagccgga
     6061 gagaggcggc gcggtgtaga gcgttggcac ttttatggaa tgcaggataa aacgggcgca
     6121 acctgcgatt ccagtggttg ggagacggtt gtctttgact gggagcaatg tatcataaca
     6181 cttgtgtcag cagagaaaac ctacgagtgg tattatggtg acggcacatg ggagtaccag
     6241 aagttcccgg agacagacta tacctgcgtg tgccgtggac agtggacgtt gggacatttt
     6301 gtattccata gctttctcat tgatgaggag gaagggcacg ttgcgatgga ctttgcatgg
     6361 aagggtaatc ggatgagtgc ccggctggta agcacttcgg aaccgccatt tgtaaaatta
     6421 aaaggcttct gggcggcgga gcagatagaa taagtgtaac aaaagattca gatggagagt
     6481 aaaaattcgg atgaagatat ttcagaaata cagaggaaag atcattctga ggagaatatt
     6541 tgcggtgagc ttcggactgg ctgcggtctg cattctgctg tcgtgtattc tcggctttgg
     6601 gtggttccgt gtgtcacaga tccagcaaca ggagagcgat tacgagaacc ggatggatac
     6661 ctatgtactg gtattgcaga attacggcaa atccatggag aatgtggtaa cagtgctcga
     6721 aaatgacgtc tatatctgca agctgatcaa ccggaataca tttgcatggg acagcaccac
     6781 ggggatcgca gcgcaggagg tcacgaatct tgtttctgtg aatccgatga tccactccgc
     6841 ctatgtctgg ctcgaggacg attatctgat caaaagcaca aatccctcct atcccatcga
     6901 ccagaacggg gatgcgcgga tgctggatat tttccggatg agcacgtttc acgagagtgc
     6961 ggtcattcca tatctggata tctacggaaa gagccagagc ctcctctgcc tgaccagcgg
     7021 atcgatggat cccaccactg ggggcaaacg cagcggcatc caagtcaaca tggatctcga
     7081 caagatcatg accttaactc tgccgaagac ggaggaggaa cagttcctgc tgattgacag
     7141 ggacgggaat atcgtttatg agcagggaac gagggagata tatcgggtcg gggaacgtct
     7201 ggaggatatt ctgtggaaaa acagaaatct gaccgaggct tccgccgaaa ttgtgcgcac
     7261 ggcgcagggg agattcctgt ccgtagctgc ggagattgat gacgaattta cgctggtatg
     7321 gctgatttcc taccgacagc tcaccggatc gatgacccgg gttggcttcc tctttttggg
     7381 aattggtctg cttgtggcga tcatcgtact gcttctcgca ctgctgatgt cgaaccgtgt
     7441 atacaatccg atcggcgaga tggtgcggac tgccagcgag gagggactgc cctccgaggc
     7501 aatggcgaga cagctggaga atacggagct gtacagcatc gcccagacct atcagacgat
     7561 ggtgcagaga ctgaaccata tcaacctgcg caaagagcag gaggatcttg ccgcgtatct
     7621 aatcagcaga gagaaaaccg caaaattgcc ggagtgggtg gaagagactt acgccaagcc
     7681 gggtgtccgg attcgtgtcg tggtcatgcg cctcagtgat atccaggatc tccacagtaa
     7741 taacacggaa gaagcgatcg cttttgagat ggagacgatc aagacgatcg tcgagcagac
     7801 gctccgtgag ttgggcaacg tcctcgtttt gccggtagac catgagttta tcgcggcgat
     7861 tctgttcagt gaggagagcg taacggagga acgtgtgacg gtggcgtcgc agcatatcct
     7921 tgaagtcacc ggagagctga tccatatcgg catggatgta gggatcagcg aagaaaaggg
     7981 cgaaacagag ggctttacgg agctaaatct catgtatcag atggcgcggg cagcgacagc
     8041 ataccgtttt atctatggta tgggtgcggt ggttacggaa ggagagatgg cgcagagggc
     8101 gttaaatgga gaacaggcac cgtcatcaga agaattgatc cagagactcc ggcagagtga
     8161 ccggacaggc ttttcggcgg agtaccgcaa gattgtcggg gagctcaaaa agctgtcgat
     8221 ccagaaagct caggatgcac tgatggagat cgctgggcag atgcagacct accggaattc
     8281 gttgcaatac cgttttgaac cgttgacgaa gaaggattac gaggtgctgg cgggacaact
     8341 ggcatcctat gaatatatgg acgatgtgga ggaatggttt ttccggatgg cagaagaaat
     8401 ctggctgatc ctcgagcgga atcggcagac gggcagagag gacgttgtgg agaaggcgat
     8461 agcgtatttg cagagcaatt atggcgatcc gaacatcagt gcccagtatc ttgcggacaa
     8521 atatcatatt acaccgtcgt atttcagccg cctcttccat gaaaaatgcg gctgcacctt
     8581 tcctgactat ctggcggcgc tccgcatcga gcgggcgaga gaactactcc tcgagcggga
     8641 gaacctcagc atacaggaga tctgtgagct tgtcggctac agcaatgcat cctattttac
     8701 gaccagcttt aagaaaaaat acggcatgac accggggcag ttccgccgga gccagcatta
     8761 aaccttagcg aatgatacta ttgagtggat gagaggaaaa agacatgatg aaattatatg
     8821 atatcacgga gaccccattg aaaatctatg gtcttgctgt ggcagacagt gagaaaagac
     8881 agtttttcaa gctgtccgaa gaggcactgg agcgcttccc acagtatggc tacctcggaa
     8941 gaagagctgc cggcggcaga gtccgctttc agacggatgc aaagaaatta tatgtaaaaa
     9001 tgacgctcgc gggcacgaaa gaggatatca atattccgct gtccggctcg gcgggcgcag
     9061 atatttatct cggaaagggt acagaagcga catatctcgg ttacattgcg ccaaagatcc
     9121 ataccgaggg cgagattaca gtggagaaga cttttgacct cacgggagag gaggtactcg
     9181 taacgatcaa tctgccgaga aacgatcatc tcctcgggat gcagatcggc atcgaggaga
     9241 gagcgaagct atgggaaaca cccgcgtata ccgtggagaa gccgatcgta ttttacggtt
     9301 cctcgattac agagggtgga tgcgcacccc gacccggcaa tgcttacacc agtatcgtca
     9361 gcagatggct ggatgcggac taccgtaaca tgggtttttc cggcagcgca cggggcgaag
     9421 aggatttcgc ggaatatctt gcgggatttc ccgagatgag tctctttgtc atggattatg
     9481 accacaattc gccgagtccg gagcatctgg cggagaccca tgcaccgttt tttgaaatca
     9541 tccggaaagc gcatcctgac gttccggtgc tcttcctcag ccgtcccgat accgatgcgg
     9601 agccagagga cagcatccgc cgtagagatg tcgtttgcgc tacctacgag gcggcgaaac
     9661 gcagaggcga tgagaaggtt tggtttgtcg acggacatga actcttcggc aagataggac
     9721 ggtctgaatg caccgtcgac ggctgccacc cgaatactct gggtttcctg cgcatggcgg
     9781 agcggattta cccggttatg caggaaatgc tgaaaaatgt ctgatctatt ttagaaaaat
     9841 atatttatag taaatctctg gtcttacata caagatacag cggaagattg gtgatgtcgc
     9901 catccttccg gtagccgcgt ttggaaaaac gtaaggctgc agcagggtga ttttctgcaa
     9961 catatctttt gaaagaggga gcggatttat cttccccggc tttcacttcc accggaatga
    10021 tatcggttcc gttttgaagc acaaagtcaa tctcgctgtt tttatcagag tagtaccggg
    10081 gagcgatttc aaattgtccc tgcaactgct gtaaaatata attttccgtt aatgctcctt
    10141 tgaacgggta gcttgatttt aacagaatgg cactgttgtc gataccggcc atatgcttaa
    10201 gcagcccggt atcaaagaca aatagtttaa actgatccag cttgtcgaag gctgaaagtg
    10261 gatgctccat tttggagaca ttatatacgc gattgagcat tcctgccgag accagccatt
    10321 cgatagcttc ttcaaaatct cttgcccttc ctccttcgcg gacagcacca tacataaatt
    10381 tctcgtttgg ctttgcaagc tgagcaacga tactgcggaa tactagaaga atacgaccac
    10441 tgttgatctt cccgttatgc tttgaaaagt cattttcata tatttcgatt aactcccgtt
    10501 gtatctggga gattcttgcc ggatccttgt atttgatcca ggaggatacg cactccggca
    10561 ttcctccgat gataaaataa taattgcatg cttcagatag acgagagtga aaaatctctt
    10621 cgatcgtttg ctctttttga atgctgtcat aataggtata taaggcagga tccgtagctt
    10681 cgagaaattc gtcaaaagtc agtgggaaaa tatcgagtag atttaccatg cctaccggat
    10741 aagattttgg ctgggctaaa atggttccca acagacttcc ggcggaaacc acatggtatt
    10801 cgttcgcttt ttctttaaag tatttcagag tgtttaaggc ctcgggacat tcctggatct
    10861 catcaaaaag gatcagagtc tcaccgggtt ggattttttc accgcttatc atggacaaca
    10921 attcgataat gcggtggggg tttttgttcg cctgaaaaat ggattttaat tcatcctctt
    10981 catcgaagtt gaaatagaca taattagcat agcaggtctg tccgaattcc ttcatcagcc
    11041 aggttttacc cacctgtctg gcacctcgca aaatcagagg ctttcgttcc gggtcgtttt
    11101 tccagtttat cagggattgc attgcatttc gcttcataaa tacaccgtcc ttttcgggaa
    11161 taataatagc ataacacatt tttctgaaaa ataaacaact gttttgcaca tttttctgaa
    11221 aaaatcaaat accaataatc acatttttct aaaatacaaa agccgatagt ctgttttgac
    11281 agattaccgg ctttttatgt gcggagacgg tgggatttga acccacgtgc cccggagggc
    11341 taacgcattt cgagtgcgcc ccgttatgac cacttcgata cgtctccaaa agattacaac
    11401 gtagattatt ttatctgaaa aataaccgtt tggcaagggg agaaacgaaa aaacctagta
    11461 gtttctattg tttgcgatgg catttacc
//
LOCUS       DAWEJW010000090        11230 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2035_length_11230_cov_46.160000, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000090 DAWEJW010000000
VERSION     DAWEJW010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2035_length_11230_cov_46.160000"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2635..2734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6508..6607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3204 a         2221 c         3056 g         2549 t
ORIGIN      
        1 gccgcgtttc ttaccatagc cgggactacg gaatatagtg aggacaagaa aatgttggat
       61 ggttatacaa tggacgtgct ggagcagcgc atcggctatt gctttcacaa caaagcattg
      121 ctgaagcagg ctctgaccca cagctcctat accaatgaac aaaagatcaa caaaacagag
      181 aactatgaac gtattgaatt cctgggagat gctgtactgg agctggtatc cagcgacttc
      241 ctgtttcggg aacacccgga tgttccggag ggtgagctga ccaagatgcg cgcctccatg
      301 gtgtgcgagc cttccctggc attctgtgcc agagacctgg aactgggaca gtttatgctt
      361 ttgggtaaag gcgaagagaa taccggcggc agacgcagag acagcatcac ctctgacggt
      421 atggaagcta tgatcggtgc catttatctg gatggtggca tgcagcctgc caaggctttt
      481 attgaccgct ttattctgtc ggatttagag gacaagcgtc ttttctatga cagtaagagc
      541 aatctccagg aattgatcca aggaaaactc aaaaaagagt tcgagtaccg gctgttagag
      601 gagagcggac cggaacacga caaaaccttc gtggtagaga tcgacatgga gggagaatgc
      661 cttggccgtg ggcagggaag aacaaaaaaa gcggcagaac agcaggcagc ttatgaggcg
      721 cttctgctat tgagggatag aggatatgta tttaaaaagt attgagatcc acgggttcaa
      781 atcatttgcc aataagatcg tatttcagtt ccacaacgga atcaccggta tcgtaggacc
      841 taacggtagc ggtaagagta acgttgcgga cgcggtaaga tgggtgctgg gagaacaacg
      901 catcaaacag ctgcgtggtg ccagtatgca ggatgttatt ttctcgggaa cagagaccag
      961 aaagccgttg agctatgctt atgttgccat tactctggac aacagcgatc accagctggc
     1021 gatcgactat gacgaagtta cagtggcgag acggatctac cgttccggag aaagtgaata
     1081 tctgatcaat ggtactccct gcagattaaa ggatgtcaat gagctgttct atgataccgg
     1141 tatcggtaag gagggctatt ccattatcgg acagggccag atcgacaaga tcttaagcgg
     1201 taaacccgag gaacggcgtg aactgttcga cgaggctgct ggaatcgtga aattcaagcg
     1261 cagaaaagca gcagcccaga caaagctgga gaacgaaaaa cagaatctgg tgcgtgtgaa
     1321 tgatattctg tccgaactgg aaaagcaggt aggccctctg gagaagcaat ccgaggtggc
     1381 taaggtctat ctgaggaaaa aggaagaatt aaagactctg gatatcaatg tcttcttatt
     1441 ggaaaataag aaactcagag aacagctgtc ttccatggaa gaaaaatata atctggccag
     1501 caatgaactg aacgagacca gtgaaaagta cgaaggtatc aaggaagaat acgagcggat
     1561 ccagcaggag atcgaaagtc tggatgtcgc tatcgaagcc gccagggaac agctgacgga
     1621 taccggtctg atgcgcggta agttagaagg tgagatcaac gtcttaaagg agcagatcaa
     1681 ttccgcccac ggcagtgaga accatctgaa caaccgtaag gcagccctgg aagaagaaat
     1741 cgccggcaaa gaaaaagaaa aacaggatat tttaaccgat aaaaaggata cggatactca
     1801 ggtacaggag atcgcagagg cggcagccat ggcaaaggcc gacctggaag ctatccagaa
     1861 caagatcacc gaactcaata ataatattga ggccgggaaa aataccatca tcggcgagct
     1921 gaaccagcgg gctaccatca agtccaagat gggtcgcttc gataccatga tggagcagat
     1981 taatatccgg aaggcagagt taaattccag actgttacgt gctaaatccg atgaggcagc
     2041 cagagaagaa acggtgaaga agctggaaga gacttttgag accgtgactg aggaactgcg
     2101 tcagatgacg gaacaggaag cggcatccga actggagctt ggcaatatcc gagagaatct
     2161 caccggactg gatgcgaagc tgcgggagac acagaaccgt ttccatcagg aacagtccaa
     2221 gttagaagcc ctgactaata ttaccgagcg ctatgatggt tatggcggta gcgtcaaaaa
     2281 ggtcatggag cagaaggaaa aagaaaaggg catcatcggt gtcgtagcgg acatcattca
     2341 ggtggaggca aaatacgaga ccgccatcga gactgcgctg ggcggtaata tccagaatat
     2401 cgtaacggat aacgaagaaa ccgccaagca tatgatcggc ttcttaaagc agaaccgtct
     2461 cggaagagcc acattcctgc ctctgaccag tatcaccaag ccccaggaat tcaagaatcc
     2521 ggaggccctg aaggaaaaag gtgtcatcgg tatggcggat gaactggtaa atacggaaaa
     2581 agaataccgc aatgtggcca aggccatgct gggacgtatc gtggtcgtag acaannnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgaaga ccgagatgca gcgcaagtcc
     2761 attgagcaga ataccgcaag attaaatatt tcccaggcca gagaacgtat ggaagaagag
     2821 gcagagagtg cacagtccct gaagctggag gaacaggaga tcgagaccaa gatcttcgag
     2881 atccgttcca gcaaggaatc aatcgtacag gaactggctg caagtgaaga actggagaaa
     2941 accactcagg aacagattct tgtcttccag aaggaactgg aaggctgcag actggaagag
     3001 agtgaagctt ccgcccatgc tggagaatgg gaagtaaaag tcgagaagat gcgtcaggct
     3061 ctggattaca aacaggccaa tgtagaccgt ataggcggcg aactggagcg tgcacaggca
     3121 gaactgaatg agatcttaca ggcactgact gaaaacgcac aggaagtgga acgcaagaaa
     3181 aacaacattc tggagattga gaagaccatc gcggcttccc atgagaatca ggatgcttcc
     3241 aagaaaaagc tggacgagga tattgcaaag aaagaagaac tgtctgcaaa gcagaaaggc
     3301 ttcttccaga gcagagaaga aatgtcagag cgcatgaatg ctctggacaa agaagtctat
     3361 cgtctgagtg aacagaagaa aaagctggaa gacaatatcg aaggccagat caattacatg
     3421 tgggatgagt atgagattac cctgagtgat gcggcaggcc ttcgcaatga agagatgaat
     3481 gatctgcccg ccatgaagcg tgagatcagc ggtctgaaag atgagatccg gaagttaggt
     3541 aatgtcaatg tcaacgccat tgaggattac aggaatctga tggagcgtta tacctttatg
     3601 aagacccagc atgacgatct ggtagaggcg gagaagaccc tggaaggcat tatcgaggaa
     3661 ctggataccg ccatgcgcaa acagttcaca gagaaatttg cggagatcag cagagagttc
     3721 gataaagtat tcaaagaact ctttggtggt ggtaagggaa ccctggaact gatggaggat
     3781 gaggatattc tggaagcagg catccgcatc atcgcacagc cccccggaaa gaagcttcag
     3841 aacatgatgc agctctccgg tggagaaaag gcattgtccg ctatttccct gttgtttgcc
     3901 atccagaact taaagccctc tccgttctgt ctgttggacg agatcgaggc agcgttggat
     3961 gactccaacg taggccgttt cgctaagtat ttacataagc tgaccaagaa cactcagttc
     4021 attgtcatta cccacagacg cggtacgatg gaacaggtgg acagactgta tggtatcacc
     4081 atgcaggaga agggtgtatc tactttagtc agtgttaacc tcattgataa agatctggat
     4141 gattagagta tgaaaagaac ttctaatcac tcagagcaaa ggctctttcg tgaagaagtt
     4201 ctaagtttca tacaagaaaa acgcaagcgt ttttctggat agaatgctga ttggaggaaa
     4261 tcggatgagt gaggaaaaga aaggcttttt tgcccggtta aaagaaggac tgaccaagac
     4321 ccgtgacaac attgtacggg gaattgattc ggtcttcagc ggcttctccg ctattgatga
     4381 tgatttctat gaagaactgg aagaaatcct cattatggga gatatcggtg tgaatgccac
     4441 caccgctatc gtggagagac tgaaacagca ggtgaaggaa aaacatatca aggaaccttc
     4501 cgaatgcaaa cagcttctga ttgaaagtat caaggatcag atgcaggtgg atgagactgc
     4561 ctatgatttt gagaaggaac agtccgtgat catggtcatc ggtgtcaacg gtgtggggaa
     4621 gaccacttcc gtcggcaagc tggccggtaa attaaaggat gagggcagga aagtccttat
     4681 tgccgcagcg gatactttcc gtgccgcagc gggagaccag ctggcagagt gggcatcccg
     4741 ggcggatgtt cccatgatcg gcggaaaaga aggtgcggat cccgccagcg tggtgtttga
     4801 tgccgtgaat gcggcgaagg cacgaggcgt ggatgtgctt cttgtagata ccgccggcag
     4861 actgcacaat aaaaagaacc tgatggaaga acttcgtaag atgaaccgta tcatagacaa
     4921 ggaattcccc aatgcccaca gagagaatct gatcgttctg gacggcacca cgggacagaa
     4981 tgctctggtg caggcaagag aattcggtga ggtggcggat cttacgggta tcatcctgac
     5041 caagatggac ggaacggcca agggcggtat cgctgtggcc atccagtccg aactgaaggt
     5101 tccggtaaaa tatatcggtg tgggagagac catcgaggat ctccagaaat tcaattccga
     5161 tgaatttgta aatgcgttgt ttgatgtgaa tatggatgac atcggagaac tgcatctgcc
     5221 ggaggaagaa gctggagaga cagacgcgga agaataataa acaggcagac caaagaaagt
     5281 gtgacagaaa ggcatgggaa gaaaaatgca ggaagaaatg ttgactttag agaaatttga
     5341 agaagcaagt gagatcgtta aaaaagttac tttagagacc aaactggtat acagtgatta
     5401 tttcagtgcc cagaccggca accgcgtata cttaaagccc gagaacttac agtttaccgg
     5461 agcttacaag ttaagaggcg cttattacaa aatgagcact ctgaccgatg aagagcgcgc
     5521 caagggactg atcaccgcct ctgcaggcaa ccatgcccag ggcgtggctt atgcggcaaa
     5581 atgctatgga agcaaggcaa ccatcgtaat gcccaccacg acacctctga tcaaggtaaa
     5641 ccgtaccaga ggctatggag cagaggtagt actgcatggc gatgtctatg atgaggcctg
     5701 cgcaaaggca tatgagctgg cagaagagca cggatatact ttcatccatc cttttgatga
     5761 tctgaccgta gctaccggtc agggtaccat tgccatggag atcttcaaag agcttccact
     5821 ggtagattat attctggttc ctatcggtgg cggcggactg gcaaccggtg tttccacact
     5881 ggcaaaactg ctgaatccga agatcaaggt catcggcgta gagcccgcag gagctaactg
     5941 catgcaggta tccttaaaga acggcaaggt aaccaccctg ccaaaggtca acaccattgc
     6001 agacggtact gcggttaaga ctcctggcag caagatcttc ccgtacttac agaagaatct
     6061 ggacgacgtg atcaccgtgg aggatgagga tctggtagta gccttcctgg atatggtgga
     6121 gaaccacaag atgatcgtgg agaattccgg tctgctgacg gtagccgcct taaagcagtt
     6181 aaatgtgaag gataagcgta ttgtatctat cctgagtggc ggtaacatgg atgtcatcac
     6241 catgtcttcc gtagtacagc agggtctgat cttaagagac cgtatcttca ccgtatccgt
     6301 actgcttccc gacaagcccg gtgagctgtg cagagtatcc ggtattattg ccggagtcaa
     6361 cggtaatgtc atcaagctgg agcacaacca gtttgtatcc atcaaccgca gcgctgccgt
     6421 ggaactgcgt atcacactgg aagctttcgg taccgagcat aagaatgaga tcattgccgc
     6481 tctggaaaaa gaaggctatc agcccaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnccg agcataagaa tgagatcatt gccgctctgg aaaaagaagg ctatcagccc
     6661 aaagtggtaa gagccaatat ctaagtttta agatttgaaa agaagtgaat gtatcaccga
     6721 taaaagccgc atattataga tatgcggctt ttgtatggaa tggagaaaaa agttatggca
     6781 aagaagacag aaaaagaact ggaaatacag agactggaca aaaagacacc cttacagatg
     6841 ctctgggatt atgtgatgat cacggtggca tcctttgtct atgcggtatc cgtcagctta
     6901 tttttggatc cgaactccct ggcacccggt ggaatcaccg gtgtcgctat tatcttaaat
     6961 cgtctgttcg gcattgagac cggtacctgg atgctgctca tcaatatacc gatccttttg
     7021 atcggtatct ggaaattcgg actgcggttt attctgtcta caatctattg tacggcaatg
     7081 acctctctgt ttacgaacct gctgacaccg gtaggagcag tgaccacaga tcctttgctt
     7141 gcctctctga ccggaagtgc cctgatggca gtatccatgg gctgggtgtt caaggccgga
     7201 tccactacgg gaggaacaga tattattatc aagctgctac gactaaagtt cccgtacctc
     7261 aagacaggag cattgttttt cctgacagat gtggttatcg tcatagcatc ggcgtttgta
     7321 ttccggaaca ttgacaaggc actgtatgcc ggtattgtgg tcattatcac atcagtggtg
     7381 ctggatgtgg tattgtacgg tcgtgatgag gcgaaactga tctatattat cagcgaccat
     7441 gcggaaaaaa tcgcagggag acttttggag gagctggaca ttggtgtaac ttatgtacag
     7501 ggcagtggtg cctacagcgg caaggagaag agcgtcatca tgtgtgccat gaagaaaaac
     7561 atttctccaa aggcggaaga gatcgtaaag gaagaggatc ccctggcatt tatgatcgtc
     7621 accagtgcca cggagatctt cggggagggc tataagagct attttagtga gaaactgtaa
     7681 aaggtcaatt attaaatttc aggaaaaaat ttgaaagggc ttacattttt taaaagaatc
     7741 ttttataata gcgtcgtgtc cgaacggaca gacgctattt tggggatacg atgaatgggt
     7801 acggattatg atttcaaacc gggcagaagc agcctgcgga ttactgtggc atagaatagt
     7861 gtaaaaccat agagagaacc gaatggaaac tgtggtaatg atataggagc tatgtgatat
     7921 gagtgataca acattgtcaa aagaggagaa acaatataaa cggctcaccg gggaaccggt
     7981 agcacggttg gtcctggaac tgggactgcc gactaccatc agtatgctga tcacgaatct
     8041 gtataatatg gtggatacct ggttcgtcag ccagctgggt accagtgcca ccggagcggt
     8101 gggagtggtg ttcggtctga tggcaattat tcaggcgttc ggattcatgt tcggtcatgg
     8161 cgccggaagc aatatcagcc ggttgttagg agctcataaa aaggagcgag cgaaagcttt
     8221 ttccgctact ggattttatc tggcggtggg agccggagtg ttgatcgcgg tgatcggtat
     8281 tttggatctg aacggattgt gcagattgct tggaagtacg gagaccatcc ttccctatgc
     8341 gagaatctat gctttctata ttctggtgtc ggcaccggca atggcatcca gctgtgtgat
     8401 gaacaatgtg ctccggtatg aaggctatgc caatcttgcg atggtgggac tggtctccgg
     8461 cggtatctta aatatggcgg gagatgccat ccttatgtgg ggcttaaatc ttggcatccg
     8521 gggagcggca ctttccacta tgatctccca gtacatatcc tttggaatcc tgttattctt
     8581 tttcctgcgt ggaaagaccc agagcagcct gacaccgaaa tattttacct gggaatttgc
     8641 cgtgcataag aatattctta cagcaggctt ccccagtatg atgcggcagg gactggccag
     8701 cgtgtcaacc atggtgctga atgcgcaggc ggcagtctac ggagatattg ccattgctgc
     8761 catgagcgtg gtatcccgta ttttcaattt cgtattcagt gtggccctgg gtatcggtca
     8821 gggcttccag ccggtatcct ccttcaatta tggggcaaag aagtattccc gtgtaaggaa
     8881 agcgttctgg ttcaccctgt gcgccagcct ggtgaccatg gcagtgtttg caggggtggt
     8941 gtatgtgttc cgcagagagc tgttgctgga attccttacg gatatggatg cctatgagat
     9001 tgcattttat gcgttgcaga tccaatgtct gacgctgccg tttattccgg tatgtatctg
     9061 tggcaacatg ctgtttcagt ccatcggaaa gggtggcagg gcgacgatct tagcctcctt
     9121 ccgaagtggc actatctaca ttccggcact gctgatcctg acggcgttct ggggaattcg
     9181 gggcatccag tggtcacagc cggtgtccga tattttatct gcggttttgt cattgccggt
     9241 ggcagtggtg ttcttaaggg gactgccgga agatggtacg gaagtataga gaagaatgag
     9301 atcccttcgt cataagacgg agggattttt atgtacagat gtatccatac gcaggaataa
     9361 gtatctgcct tgatctgtga agctgttgac attaaataat ccgcatgata cgatacattt
     9421 aacaattagg ttaaatgtta aacgtttgga tgaaaggatg tgattcattt ttgggaatac
     9481 aggatacatt gaaagcgctg gcagatccta taaggcgtga aatactaaat cttctgaaaa
     9541 acggtcgatt gtcggcagga gagatctgcg aacattttcc ggtgacgggg gcttccatat
     9601 cgaggcattt gtcggtgcta aaggaggcag atctgatccg ggacaagcgg gaaggaaagt
     9661 ttatttatta tgaactgaat gcttccgtgc tggaagaaat catgttatgg atcacagatt
     9721 tgaaaggagc attggataat gaagaaaatg atacagaaaa ataaaggcag gctgatctgc
     9781 tcggcatttg ttatgtgcat aggaatgata ttgggatata cctgtgagaa cagtctgtgg
     9841 gtaaacggca cttttgcagt gacttatgtg gtagcaatgg cagtaacttt ttacgacaac
     9901 agaaacaggc agcagagtga taaggtcata agaatggtta cgtggatcgt gccgggaatg
     9961 acattgctct ataacggtat cgccagatgg attgatatgg gtgtaaggaa agagaatctg
    10021 ctcatggtaa ttttgtatgt cggaatggga ttgctatttg ttattgtggg caattatctg
    10081 cccaaggtga aaccgaaccg caccatcggc atccgcgtcg tatggacatt gcaggatgag
    10141 gagaactgga atgccacgca ccgcttcagt ggaaaaatat gggtggcagc aggactgttg
    10201 agtatgagct gtggactgtt cagtgacagc atggctgcat tatttctgtt tattgtcgct
    10261 atcacggcag cagcttttgg cagcatcctg tattcttatc tctattatag aaagaagctg
    10321 cgcaccggaa aagggctggc agtacactgc aaccataaag tagttgcagt ctatgtgttt
    10381 ataatgctcg catgtgtgct atttacggta tggacgttat atacgggaaa aatcgagatc
    10441 tgttacggag aaaatgaatt tactgttcag gcggagggct ggcaggatta tacagtgaaa
    10501 tatgacgcta tcgacagcat tgcttgcgag gagaatatgt tccgggatac gaatacaatc
    10561 cggacgaacg gttttggcaa cctgaaatac tccatgggac atttccgcga tgaaatccat
    10621 ggagattaca ttcgctatac ccataacgac tgtgatattt atgtagtcat ggaagtggag
    10681 gggagcaccg ttattctaaa cggtgcagat gatgcgcaga cccgggaaat ctacaataat
    10741 atcagagaac ggatggaaaa ataatatctg taaatatatt tcccaattgc ttttatattt
    10801 tttaaaaatg gtatacacta aacttatgta agtttgtaaa cgaggatttt tggaaagggt
    10861 ggttacagta tgctgaaaca gtggatcccg gcggcaaagc cggacaaaga ggaactggaa
    10921 aaacgactga atgcggcgca ggaggagctt ggtagactcc agatgcagat caaggagcat
    10981 aagctcccgg tgctggtgct gatcgagggc tggggtgcgg ccggcaaggg cagtaccatc
    11041 ggcggaatta ttaaaaatat cgatccgcga ttttttaagg tggcttccat gtctgcaccg
    11101 acagaggaag agaagcggaa gccttttctg tatcggtatt ttgtgaaaat tccggaagcc
    11161 gggaagttcg aatttctgga ttccggctgg atggatgagg tgacgaaggg aagacttcgg
    11221 ggcgaactgt
//
LOCUS       DAWEJW010000091        11154 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2050_length_11154_cov_48.118389, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000091 DAWEJW010000000
VERSION     DAWEJW010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11154)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2050_length_11154_cov_48.118389"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2534 a         3113 c         2308 g         3199 t
ORIGIN      
        1 cggaaatggt gaatgaatat cggacgccga ttgtgattgt ataaatagaa agttagtttg
       61 gtgtaaatag tccaaaaacg atgaattccg tacaaaaaag ttcctaaatc aggaattttt
      121 gtacggaatt tctttatttt gctcatgcag cgaaaataga gactttttac atataaaaat
      181 ttcacatttt tgtatttttt tcgcagaaat tggccgtttt aaatacaaac agaactcccg
      241 gagtagcaga aggtcctcag gcagatgtct gtcggagcgt gttgatattt tcttaagcag
      301 tggtgagcca tcagggtgga gcaaacacga tgtttgcgac aggcggccgg cgacatggat
      361 gtcgcctaga gccgtgcccg ttcggatgag atacacttta tccactgctt tagaaaattc
      421 acactcggag accgatatct gtctgagggc cttctgccgc ttcgggagtt cgtctggtct
      481 aaggctccct ccgctacgaa ccatgcctat tcataagatt gtcagcaaag aacgctgtca
      541 atcttatttc atagacggct gtcgccgtat aaaaatagaa cgaaataacc tcttgtgtga
      601 gcaagctccc accgaggtat tccgttctat ttttgcatgg ctcgtagcta ggctaacttt
      661 ccagttagtt cttaaagcta tgaataggtg cggggatacg gccaccgcgg ttgacgaaag
      721 catcacagga gaaggtattc acgggcatga tgggcgcgta acctaacagg ccgccgaact
      781 ccacggtctc tcccacgcct tttccgatta cggggatgat tctgactgcg gtggtcttct
      841 ggttcaccat accgatggcc atttcatccg caatgatacc ggaaatggtg gttgccttgg
      901 tgtctccggg aatggcgatc atatccagac ctacggagca gacacaagtc atggcttcca
      961 gcttctcgat ggtgagggct ccggcattca cggcatcgat cattccctga tcttcactga
     1021 caggaatgaa tgcaccactt aaaccgccga cgtaggaaga tgccataaca ccgccctttt
     1081 tcacctgatc attcaacaac gccagggcgg cagtggttcc gggagctccg gcatgttcca
     1141 gaccgatctc acacaggata tccgctacgc tgtcgccgat ggccggggtc ggtgccagag
     1201 acagatccac gatgccaaag ggaacattta acatccggga tgcctcctgt gctaccagct
     1261 gacctacacg ggttacctta aaagcagtct tcttgatagt ctcacagaga acctcgaagt
     1321 tctcgccgcg caccttttcc agtgcggtct ttacaacgcc ggggccgctg acaccgacat
     1381 tgatgatctt gtctgcttcg gtcacaccat gaaaggcacc tgccatgaaa ggattatcat
     1441 ccggtgcatt acagaatact accagctttg cacagcccag ggaatcatct tccttggtat
     1501 actctgcagt ctccctgacc atctcaccca tcagctttac ggcatccatg ttgataccgg
     1561 tcttggtgga gcccaggttc acagagctgc acacacgctc tgtggtagac agcgccagag
     1621 gaatggaacg gatcagcagt tcgtccgccg gggtcattcc cttgctgacc aatgcggaat
     1681 aaccgcccag gaagttcaca ccaacctcat gtgctacctt atccaatgtc tttgcaatac
     1741 ttgcaaagtc ttctatcgta tgacatgccg caccaccgat cagtgcaatg ggagttacgg
     1801 aaattctctt atttacaatg gggatgccaa attctctgga gatctcctct ccggtcttca
     1861 ccagatcctt ggctgcttcg tagattttgt tataaatctt ctcatttaat ctgtccagat
     1921 cggaatcgat acagtctaac aggctgatac ccagtgtgat ggtacgcacg tccaggttct
     1981 cttcctcgat cattttgttg gtctctgtta cttcatataa attgatcatt acggctccta
     2041 tctgcccctt cgggtcttat ttatttcgaa atcgtggggc ttatcagatg cggtgcatct
     2101 tgtcaaagat ctcttccttc tggcacttga tcactacgcc aatctcttct ccgagagcag
     2161 ccagttcatc tgccatatca ccaaaagttt tctgcatctg attggtgtca acgatcatca
     2221 tcatgttaaa atatccctgt acgatggtct gggaaatatc caggatattg atgccgtttt
     2281 ccgcaagata ggtacatacc tttgcgataa tgcctactgt gtccttacca actaccgtaa
     2341 taattgttct tttcataatc gccctcattt tctgcatgaa atcatgctat tatttttatc
     2401 tattaaacta cactttacta caatttgcaa cctcaagttc agcctatgaa catattcaga
     2461 cacagaaatc atgcttgcat cattttatga acaaatatgt ttcaggcacc gagcgccaaa
     2521 ctatggagca aagctagtca tgaaatgacg caaaagcgtg ctatgatgcg gctccaacga
     2581 ataatgctgg ctgatgcgct gcgcatccgc tacgcaatat tgatcaccgg agatacattg
     2641 ctcctgtctg ccaccatcat ccggaagtct tccgcccggt aagggttctc tcccatggtg
     2701 atttccatcc ggaccgcttc ataggccagc tccggaagat tattctcctt gctgagatgt
     2761 cccagcacga tagcctgcag cttgtcatgc aggattcgac agagcagctt gccggaattt
     2821 tcattggaaa ggtgtcctct gtcccccaga attctctgtt tgagataata gggataaggt
     2881 cctacctgca acatcttcac atcatgattc gcttccagca gcaaggcatc cattcccttc
     2941 aggcactcca cggtgtaatc attatagact cctaaatccg tacagattcc taccttttta
     3001 tctccgtaag caatacgata gcccaccggc tgtgccgcat catgggagat tttcatggga
     3061 ttgacagtca gatccttgat agtcagtttc acatcttcct tcacttcccg aaacagggct
     3121 ggatcgatct tgcccagatt ccccatcttc tggatttctt ccagggttcc cgttgtggca
     3181 tagatcggca cttcatattt tctcgccatg acacccagtc ccgccacatg atccatatgt
     3241 tcgtgggtca ccaggatgcc atccagatct cttccggtga gtcccagact gttcagcccg
     3301 gcctccgcct ttttgccgct gattcccacg tccaccagca gatgcgtggc ctctgatccc
     3361 acataaatac agttgccgct gctgccgctg gcaatactac ataatctcat aacttcttaa
     3421 actcttttct atcttacttc tgtcttactc ttttccgttt tccatcttcg gcattaatgc
     3481 atactagtgc atgcttttcc agtagatctc gatcacatct ccctctttca aaggttccat
     3541 gaactgtgcc gggcgtccat ttaagttcgt cacaatggat ctgcctgcgg atgcggagga
     3601 tcccagatca aaatcaatat aatcaaacac atccacaaat acataggaag ccttaccctg
     3661 catggtgatg ggggaatggt tcacgatcac tgttaatgga tgcggcccct gtggtgacac
     3721 gctttggctc tgtgccgtct gttccacgct ttcgtacttt tcgtcctgct tcccggaagt
     3781 cccctgcgtt gtcacggaag ccgtcccgtt ttctgctgca gtctctgtcc cctgtgtcat
     3841 cgccgcttct cctgccacag cctctgcctt ctgcatcaca ggcccttctc cataaacagg
     3901 ctcttctcta taggcatcct cttctgtgtc ctcatccggc aggtctgcat aactcccttc
     3961 cggcagcggg ttcttcatat cccagcttac ggtgaaatgc tcataaactc tggtatccgg
     4021 ctgtgccgct gtatcattga ccctgatatc cgcaccgaga ggcacatcca gaaattctgc
     4081 gatttccttt acagtataag aattccgaat ccgaatatca tcgttctggc cgatccggta
     4141 gaattcattc tctctgtgac cgttgacttc cgctgtcttc ggcagactga tccgtttacc
     4201 gttcacatag acctgcagtg catcccccag ttcagagagt tttcccagct ccaacaccgc
     4261 cggttcgccc tccgtggaag gtgttaccac aatgcggtcc ccattatgta cctgggtata
     4321 catatccgcc tcgtctccgt tgacccggat gactgctgcc tctccctgtt cgccccgcac
     4381 catacgggtc ttaccattga cagtaaacag aagggcttcc ccttttctgg ggaacagctg
     4441 gtcattgggg aactgcatct gcatggcggc atctgtcacg gataatttgc cgttatcata
     4501 gagctttacc cgctctccgt taaattccac gaaaataaaa ttattgctct gtacataata
     4561 gcttaaacag ataccgatag gtgttaccat catggcatcc tttctggcat tttccagttc
     4621 aaatgtaatg gtctgcatga cttcctgtcc ccggattgcc acacgttcct tcacgatgcc
     4681 cagcttttct gccagcttct ccgtgtaacc gggcaccatt ccaccgccgc ctaccacgaa
     4741 cacggcactg accggtttgt ccccgttcag ttctttgatg gtatcggata ccagctgggt
     4801 catacgttcg atctccggat ccagcagttc cagcacttcg ctggctttga tggtctgggg
     4861 cagtcccatg atatcttcgt attcgatagt ctcctgggtt ctgcattttc tcttgatctg
     4921 ctcggccacc tcgaattcca cgagacaatg ctgtaccaga atatccgtca ggctgtctcc
     4981 tgctacgggg atcatgccat aggctgtgat ggttccctct ttggtaatgg aaatatcact
     5041 ggtgcctgca cccacgtcca ccaatgccat atttaacata cggaacttct ccggaattgc
     5101 tacctgaatg gcagcaatag gctccagtgt cagattcgcc acatgcaggc ccgccagttc
     5161 tactgcctta tacagaccgt ccactacatc atccggcaga aacgtagcga tcagatccac
     5221 tgcaatgttt tttgccttat gtccctccag attacccatc tggtagccat tcatgtaata
     5281 ccgcatggcc gtataaccca cacagtagaa tttcatatct gtatccgtat tgctgttctg
     5341 gaattcctcg taagccttct ccacgcccat ggtacacaag gaatacacat cttcctgcgt
     5401 gatctcccgg tcactctcaa agctgtgctc cacataggta gtgacggtac gcaacacacg
     5461 gcctgcagct gcgatacata cttccgtaag ttctctgccc agatccgctt ccagctgttc
     5521 ctttacctgg gaaatagtct ctcccacctt gccgatgtca tggatctgtc cgtccagcat
     5581 ggctctggtc tcatgctcct tggagcgctg tgccagcaca tggaacttcc cgccatttaa
     5641 gtatcctacc gtacctacga tgcttctcgt accgatatcc agtccgaata ccagctgtcc
     5701 ctgtttttct tttactcctt ccatgaatat gcctccagtt tctgtctgat ctgtctgtag
     5761 ctgtcttcca gactgtcact gttatcaatg acaaaatcac aatgctgccg gaattcttcc
     5821 tccaaaagct gtgatgctat gatcttttct atcttctccg gtgtgtaacc gcggctttcc
     5881 gaaagtcttc ttctgcgtac atcttctctg gcgtagatat accacatttc atccactata
     5941 tgttcatacc cgctctcgat caaaagtgcc gcttccacaa aaaacagttc cacatcgccc
     6001 ttattctttg cctctgccag acgatccgcc agatattttt ttaccgcagg atgaatgatg
     6061 gcattcacct gttccaacag tgtcggatct gcaaaaatct ttgccgccat tgccggctta
     6121 tcgatctgtc cgtccggcgc aaggattccg tctcccatca gcgctaccag cgcatgatag
     6181 ccttcggttc ccggttcctt gactttatgc ccgatctcat ccgccagata gatctcacat
     6241 ttataatgct gtccgatata ttttaaaatc tctgttttcc cggcacccac accgccggta
     6301 atacctataa aacgcattta tttcgcctca taccagttat tgcccgtgtg cagatcgatt
     6361 tccagcgtca ccgccagatc tgccgcggac ttcatatttt ccgtaaggat ctcacggact
     6421 cttgcctctt cctcccgtgc ggtctcgacc aacagttcgt catgcacctg caggatcaga
     6481 cgggatttta acccttcctc tttcaatgct ttccatacct tgatcatggc gatcttgata
     6541 atatccgccg ccgttccctg aataggggca ttcatggcca ctcgctcgcc gaaggaccgt
     6601 tgcatgaaat tggaggatga cagttccggg atagggcgtc ttctgccaaa catggtctcc
     6661 gtatagccct tttcctttgc atccgccacc agtttgtcga tgaattcctt caccttcggg
     6721 taagtggcaa aatactgttc aatgtactgt gccgcttcct ttttgctgat actcagatcc
     6781 tgactcagtc caaaggaact gataccgtag acgataccga aattgactgc cttggcattc
     6841 cggcgctgca gatccgtgac ttgctcgaaa ggagtatgga ataccttgga tgccgtgatc
     6901 cggtgaatat cctcttcctg tctgtaagca tcaatcagct gttcgtctcc ggacatatga
     6961 gccagcacac gcagttcgat ctgggaatag tccgcatcca tgaattcata gccttctctg
     7021 gggataaata ccttgcggat ctgtctgccc agttccatac gcatgggaat gttctgcaga
     7081 ttcggctctg tagagctgat acgccccgtg gcagtaatgg tctgattgaa attcgtatgg
     7141 atcctgccgt cctcttcaat aaaagctgcc agtccgtctg cataggtgga ctttaacttg
     7201 gtcagacctc tgtattccag gatatctctt acaatagggt aatccgccga cagcttttcc
     7261 agcacatccg cagctgtgga atatccggtc ttggtcttct tgccgccggg gatctgcatg
     7321 gtctcaaaca gtacctcgcc cagctgcttg ggggaattga tattgaactc tgttcctgcc
     7381 tgtgtgtgaa tggactgttc cagttcctca atgcgggcca ccagtgcatc cccataagcc
     7441 ttcagttcct cccgctttac agccacacct tctttctgca tgtcatacag taccagtgaa
     7501 agaggcatct ccatctcctc catgagacat ttcatgcctg ctgcctccag cttgtccttc
     7561 aggacttttt tgcctttccg ggagacatag gctccgtagc acgcatactc catggctttc
     7621 ccggcgtcct cggaaagcag tgtccgaaaa tctctcttgc cgaatacttc cgctctgccc
     7681 gggatcagaa ttcccagatg ctccgaagca atggcctcca tgtcataatc attcttcaga
     7741 ggattcagca gatacgccgc gatcaatacg tcaaaatact gttctgtact gctgcggtcc
     7801 agatagtcat actggtgctt cacatcaaag gtagcgatct gtgtatccgt acctgcggaa
     7861 agttcctgta gagcttccgt gatctctgcg gtgtcatcca cagattccac cgcttccgca
     7921 aacagactta actgcccttc cggttccttt tcatgggtca gggcataaaa taccgtctcc
     7981 tcatcggaca cagacaatgc cacccccagc aattcttctt ttttcgtagt agcatccact
     8041 ggttccgtga tcagccacag gccgatctcc ttctgtttct gcgctttttt cagatattgt
     8101 aaaaattctt ctttatcttc aatctgtcgg aagctttctg cgatcttgct gtcattggcg
     8161 gcggcatctt tttcaaatct tcccaacaaa tttttgaatt ccagacgttt gcacaaggta
     8221 taggcttccg gggtatagaa cccctctgct ctggcctgtt cgtaatccaa ctgcaattcg
     8281 cagtcggtct tgatcgttgc cagcgtcttg ctcagatctg ccagatcctt atgctcgatc
     8341 agggattccc ggatactttt tttcgagatt tcttccacat gggcataaat attatccaga
     8401 gaaccgtact ccaccatcag tgcctgtgcg gttttctctc ccaccttcgg cactcctgga
     8461 atattatcgg cggtatctcc catcagggct ttcagttcga taaactgtaa cggagtcacc
     8521 tgataggcag ccagtacatc tgcggcataa taatcctcga tctccgttct gccctgcttc
     8581 gtcttcggaa tccggatctt gatatgatcc gtggcaatct gcagcagatc tctgtcaccg
     8641 gagaccagtg atacttccaa tccggcctgc tctgcttttt tggccagcgt tccaagaatg
     8701 tcatctgctt ccagtcccgc ctgctccata atggtaatat gcatggcctt caggatttcc
     8761 ttcatgacgg gcacctgctc ccgcaattcc tccggcatag gctttctcgt tcccttgtaa
     8821 gcctcataca ttttgtggcg gaaagtcggt gcatgaacat caaaagccac tgccagatat
     8881 tccggctttt cctcctccag aattttaaac ataatattca aaaaaccata aatggcattt
     8941 gtgtgcagtc cctctgcatt actcagatcc ggcactccgt agaatgcccg gttcaatata
     9001 ctgtgtccgt cgatcaatac cagtttctga ctcatactct gtctatccta tcttaattct
     9061 gtcctcttga ttctatcagg tgtcgctaag agatgatcca tcccagaaat cccataagaa
     9121 aaatcaccgt cagcacttcc agtacggccc ctgtcttcag aaaagccata tgaaatctta
     9181 ccttgcgctt tgcttccgca agtctcagtt ccagttctct ctgcaggtca aaagcatcgc
     9241 cgtcttccag acgctgcata ccttccgtta caaggaactg aatcaccaat tcctccttgc
     9301 agcccgggca ttcccgcata tgctccccga attcctgcaa ggtctgaaaa tccagttttc
     9361 ctgcaatgaa atcaggaata ttcttttcaa attctttgca attcatgctt ccaccctatt
     9421 ccaaaacagt tctacagatt gcgttcccat atactcatac acttctacta tacaacatct
     9481 tgtgtaaaaa gactagaaaa atagtagcaa gattccagaa aaaaactctt gtcaactttt
     9541 ttaatctatg ataagataat ctctgttgtg agtgtttgaa tgccggcgtg tcggaatggc
     9601 agacgaggca gactcaaaat ctgttggtgg caacaccgta tgggttcaag tcccattgcc
     9661 ggcagttagt gcagaatcga atcggttctg cgctttcttt ttgtattttt ataaccacat
     9721 cgcatttcct gctttttcta atttttttcc aatcatctcc tatggattaa ttgttacaaa
     9781 attgttacaa ttcatttgtg cttagctaac acagacgtaa ccgaacacca ggcaacagga
     9841 aaaggaggaa gaaagtatgt gtttcaaatt cattaactgt tctcagtggt tgaatgcatt
     9901 ctgcggctac ttcaattttg gctgctaata tcgttttcga cgaatagagc gttccataga
     9961 cgcacgaaaa ggctctcctg tttgggatga gccttttctt atttctacac tttttctatg
    10021 aatttataca tttttaccga tagtcataac ggagggtgac atcattgccc tgtatctgta
    10081 cttccgcaca gcttccggtg acaatcggca tcatgggtgc cagatgtccc agatccacat
    10141 ccatgatcac gggaacattc ttctctcccg ccacggccag aactgcctgg tactgatcca
    10201 gtcccatcat ctcctgtccg aaacacaacg gtcttccgat gagaaaacct ttgacatact
    10261 gaaaccatcc ggcttcctcc atctgccaca tggctctgcg gatggcaaaa acattcaggt
    10321 cacaggcttc caggaaccag atgattccgt cctctttata cttttccaga aaatcctgtg
    10381 ttttatcaaa ccgggttccc agtagattca ccagacaatc catacagccg cccagcaatc
    10441 gacctgaaaa gcggatctcc ggttcctctt ctgctgtctg tctgccaaga tactttttca
    10501 gcactctgga ttccgtggtg ttatagggca acagaggctg ttcttcattt ttcagagact
    10561 ccttttccca cttgtcatat ccatgcacct ctttggtctc tccggtcaat attccgaagg
    10621 catctttcag ggatgcatgc cagggctcca tgccaaaagc ggccgcgcag gggccgtaga
    10681 cggaggcggt atcacagatg gttgccagca gataggtcat gttggtattg tcagaatacc
    10741 ccagatacca cttgggctct gctgcctgca gacgctcaaa atccacatgg ctcatggtct
    10801 cgcacatcag ttctccgcca ccgcaggaga tcagacagtc attttccggg ctgcaatagt
    10861 aatccgtcag ctcctgcccg catttttcgg gggtgttact gataccgatg ccctctccgg
    10921 cataacagtt ggggcctaat tgtaactgat agcccatttc ccggaatttc ttctgggcat
    10981 tgccaaaggc agtgtaataa ggttctatct gacagccaaa agaaggggcc acaaatccaa
    11041 tggtccctcc ccgctgtata tgtttcggat atttcattgc ttcgtatcct cctatatcgc
    11101 ccattcgcat tccgggctat cgcccttctt gctcatgttc gttcacttcc ctca
//
LOCUS       DAWEJW010000092        11083 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2064_length_11083_cov_53.506347, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000092 DAWEJW010000000
VERSION     DAWEJW010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11083)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11083)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11083
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2064_length_11083_cov_53.506347"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3238 a         2204 c         2982 g         2659 t
ORIGIN      
        1 gagagagctt ttgggatgct atatacccgc tgattttttc gttacacaaa aatcgcattt
       61 cgttacatag gattgaattt caaaggaaaa gtggcgatat aaaaaaggaa atgtttgaat
      121 ggaataagat ccgggggagt agaagacgaa tgctgaaaat agcaatatgt gacgatgaac
      181 agcccataag agaatacctg aaaaggctgg tagagaaatg tacggagggg gaaatccgac
      241 tgtttgccga cggcggagaa ctgttggcgg atccgacggc gtttgacctg atcctgttag
      301 atatttccct gaaccgggag cagaatgcgg cggaaccgga cggcatggag acggccagaa
      361 agatccggga aaggtcggat gcactgatta tcttcgtgac ggccttacgt gaatatgtgt
      421 ttgaaggcta tgatgtgggg gcgtttcatt atcttttgaa gcctatcgat gagcagaagt
      481 tcacagaagt catggacagg gcaatctgcc agatccggag taaaaagaat gttgaaccgc
      541 tggttataaa agtaggcggc aactatgtca ggattcctgt ggatgatatc ctctatgccg
      601 agaaccaggc cagaaaaatt atgctgcaca ccaagtcaaa gaaggagccc tactgttttt
      661 atgaaaaaat ggaagtgctg gagcagaagc tgggagatcg ctttttccgc tcccatcggg
      721 gatttcttgt gaatctgcag gaggtggccc ggtatgacaa cagcaacata gaattgaaaa
      781 acggtgagag cgtattttta gcaaaacaga aatacaatga ctttgtcacc gcatacatga
      841 agtatctgag aaaagtgtaa gcggtgatgc ttcagaaagg aatcagcggg tgatgggcct
      901 gctgattttt tgatgtttcg ttacatgaaa agatcggttc gttacaagac atattaaggg
      961 tgaaagaaaa gcggcgatat agaagacaga gagaaaaaat tgcagatcaa aggaggtcgc
     1021 aaaatgagga tcacaaccca aatgctggca aattccgcag caaaaagcgg gattcccatt
     1081 cagagaacgt cgctcttgga cattatcaat aacaagtcat cccagaacct gctgggaacc
     1141 atcgggaaga gcatgaacac cacaagtacc ggtgccacgg acattctgag gaaaaataat
     1201 tatgcaaagc tggaggatct gtcggagaag ttgagccaat ctgcgtcaaa gcttgcaggc
     1261 agcgataagg attccatcta cgacaaggca aaagagagcg gagacaccac ggacatcctg
     1321 agtgatgtga acaaaatggt ggaagcgtac aatgctacca tgaagcagct taagaccacc
     1381 ggcggaatca tgaatgaatt ttaccagcaa cagctaaaaa acattccagc gggcagtaaa
     1441 gaatcactgg aaagtatagg aatcagtcag gcaaaggacg gaagcctgat cgtggacgag
     1501 aaagtattcc ggaatgcgga tgcggatacc ttacagaagg tactgggagg agagaatggc
     1561 atcgccccca aaataggatt tctcggtgag cacatcaata agaatgcttc cgaaaatgtg
     1621 gtcagcgcat ccaaccggta tggttccaat ggggcaacct atatggaagc ttttgaggct
     1681 aacaagtata atttctttgg gtaagattgt gtcgtaaaaa aggcattgaa tttcaaaaaa
     1741 aattgagata cagtgccttt ttgcatatct gaaatgcaga ccgaattctg gcattaacat
     1801 ttctgcatca attcaaaaac atttctacaa aacttccggg atacataata tacaggatag
     1861 gaaaatcgaa gtataataaa taaaaaagta taggatacct cggaaaggga taacaaatga
     1921 gaatattaga aattatattg ccggtactta tcatgctggt tctcggtatg gcatgcagaa
     1981 aattaaagtt acttaccaaa agcggaatag ataatatgaa actgctggta accaatatca
     2041 tgcttccggt ggcaatcttt catgcactgg ccacagcgga ttacagtgtg gagacctgga
     2101 aactggtggg gattatgttc gttatgctgg tggtatcttt cggagtagga tttctgttga
     2161 agccgcttat gaacggagaa taccgcaagt acctgccgtt tatggtcagt gtatatgagg
     2221 gtggcctcat ggcgtatcct ttgtatacca gtctgtgcgg gcaggaaaat ctctcacaga
     2281 tcgcagtact ggatattgca ggacttttgt ttggattcag catctatatg gggatgctgg
     2341 gacaggtgga aaacggagag aagatcaatg caaagaaact atgtatgagt gcattccaca
     2401 caccggcatt tattgcaagt gtgcttggaa tcctggcagg actgtccaag gtggtgattt
     2461 gcctgatcga cagtcccttc ggcggtgcat atctggcggt ggaagggatc cttaccacat
     2521 ccgtgacagc aatcattctg atcgttgtgg gatatagcat ggaactgaca aaggaactga
     2581 tccggccatg cctgaagaca atcctaatga gagttctgct acagacactt atggcaattg
     2641 gagtgctgtg ggcagtgcat ttgtggatag gagataacat gctgttgaat cttgccatca
     2701 tttcctatat gtcggcaccg gccactttca gcatgcagac ttttctgaaa aaggaagagg
     2761 gcagtgccta tgtatcaaca accaattcta tgtactgtat ggtgtcgatt ctggtatata
     2821 tcattctggc agcagtggtt tattagaagt gtgaaaaagg caagcggtat cctgctgtcc
     2881 ggtctggttg atgacaagaa tgcagggggt attttttaaa tcttcggttt ccagggaact
     2941 taatttactg agtggatttt tggagggaag ttcaatataa attttacttt ctgccagcac
     3001 ttcattatta tacgcatccg aaaaagctct tctctggttg ttgatggcaa gatcaacagc
     3061 gtcatttttc catagcatga tacaattctt catgacttcc caccataatt tttacttcta
     3121 tggtaggata ttaataactt aatctcttga taaataagtg gtcgtttact atagtaaaaa
     3181 gtaatcggtc acttatttta ttctgaggaa aggaaaaaca tttatgataa aacctaaccc
     3241 cgattcctgt catctcctgt tggacagcag gcttgcgaac gaggaagtac agaaaaatcc
     3301 atacacttac aatagcatca gagaagtcct gtcggatgga gcactaaacg cagcaactgt
     3361 ggaacacccg gtgacggtct acattgctcc cggcatctat tggctggagg atccgcagtc
     3421 ggaggtggtg atcgtaaggg aggatccgaa ggatctttac ccttacggat gcaaggtaaa
     3481 ctgcgcaaat ctgaaattgg tggggctgtc ggagaacccg gaggatgtgg tgattgccgc
     3541 caaccggggg aacgaccatg gagcgaaggg gaattatact ctgttccatt tttccgggga
     3601 acaattggag atggaaaacc tgactctggg gaattattgc tgtgtggatc tggattatgc
     3661 attggatcct gcgcagtctg tgaaaaagag gacagaagcc attacgcagg cacagctggc
     3721 ggatacgaat gccgataagt tccatgcgaa gaattgccgg tttgtcagtc gtctgaacct
     3781 gtatcccgta tgtggcgcgg gcagaagcct gtatgaacat tgtcattttg aacagacgga
     3841 cgatgctctg aatggaaatg cagtgtacct ggactgtgag tttgattttt acagcggaat
     3901 gccgatctat caggcttccg gtacgggagc ggtattctta aactgtacat ttcattgtaa
     3961 atatccacag gatggggaga ctcacgcaca gtattttaca aaagtcggag gacagatcgc
     4021 actgatcgac agcagcttcg caggtctgcc ggacaccaaa gttgcggtct tgtggacgaa
     4081 atatccttcc gtagcattga agtgctatca ggcgaatgtg acatatccgg aaagccggtt
     4141 tacgcctccg gaggttgcag attcccatac ggtggacatt gacgaaaaga tgctggcaga
     4201 ggcctactat atccgtaagg atggagaaac gatctacaat gtctataatc tgctgggcgg
     4261 gaaagacgac tgggacccgt tggggaacgg agaagtgatc cgctttgccg gaaaaacaga
     4321 tattcctaca cagcttctgc tagagtcaga ggcttttgaa ctggaagcag gcggatcttc
     4381 cataaatata aagggaaaat gcctgacctt tgatgggcgt gagaggaagt gtgagataca
     4441 ttttaaaata gaaggagaca gtgcagactc catagaaata cagcgtgtgt cggaggggag
     4501 ctgtctgctg caactgaaag acagtaatat agaccatgag acggaagtag tactcacggc
     4561 gcagaccaaa gagggattgc agaccggtgc gtatgtcaga attcatcccc ggaaagtggc
     4621 ggcaccaagg cttacgggca atccggtgat ctgtcttgaa ggcaaaatgc tgcggctgtc
     4681 ttatgatttt acggaagcgg agaatgactg ctcggatatt atctggtatc gaagcagaaa
     4741 cattcgggga gaggacaaaa tcgtgacggc aatctcccaa ccggatcagc cggagaaagt
     4801 ttatgccctg accggagatg atgtgggata ttatattttc gcacagataa gacccagaac
     4861 gaatcgcagc gaatacgggg aagcagtgca gtgcttttat gaaaaagcta tttcgccgga
     4921 agatgtggaa acagacagga tctggacaga ttttcataac cttcctcttt acagccatgc
     4981 cggaaacgaa aaaggtgtat ggaattttga tgcgaagcgt ccggcggata cctgtgattt
     5041 tgaaaagtgg gatcgggaaa agacgcaggt ctcctggcac tacggtgcaa caggggacgg
     5101 cagtaagggc gaagggctct atcagggaat gcagggggcc aggatccgct atacgccgac
     5161 cacggcaccg gagatgggaa cggaaaccaa acggaatatg gaagtcttgt tggaagccga
     5221 tccggcaaag tctgccggac agggttttgg cagcgccgga cagtatctgg atgtctgcat
     5281 caagacggac acggacactc tggacggtta tggacttcgg atcatccgta cggcggccca
     5341 ttccgatgcg gtatccatgt atctgataca gtatgtgcgt ggacaggcgc agtgcatttc
     5401 ccgcgaggtc gtgacaaact gctttgtaac gggatgcaga atttgggtgc ggtatgagaa
     5461 cggaatatta tcggcaaaag catggacggt tacggaacct acggtggtac agcaggaaag
     5521 gggctatgcc cggggagtgg agcttacggc agaagtaggc agaagagaaa atgccgaaaa
     5581 caccggacta ctgatctggc acaccggatc gttgggaacg gagaactgga gaaataccac
     5641 gatgttacat ggcgtaagca ttctttattt ttaaaaattt cagactgcat cgggcaaaat
     5701 gaaagatgaa ctggtcgaca gtacctatct gcggctgaaa gcatgtgcta catcagtgga
     5761 gcagtatttc acatgggata tccgtgagga tattctggaa aaggatgata tcagttatca
     5821 gtttatagat tctttgcagg atgagcatat tgaacagacg cttttcatgg aggatgagag
     5881 atttattact tccattgtgg acaaggatgg gaacagggca gaaggaacca aggctgatcc
     5941 acagatatgg gagacggtca gagccggtaa cgattatgaa gctgatggag tagagattca
     6001 gggagaagaa tattatgtct attatatgcc ggtatgttcc gatgacggag agattctggg
     6061 aatggctttt gccggagagc gggagagcat agttcaggat gcaatatccg acatgttaag
     6121 aagtctggtt ctgcgctcag tatgtctgaa tgttcttttt attgctgtca tggtttatct
     6181 gtcattcaaa atacgaaaac cattactgac gaccgcagaa tatattgacc aaattgccaa
     6241 tggtaatctg tcaggcaatc ttgaagtgaa atctgtgatc cgtgaagtga ccactctgat
     6301 ccgggcatct gtcagcatga gggataaatt gaacagtatt gtcaccgagg tggatggaca
     6361 tgctgcacaa ttggatagca gcatggaatt attaaataca ttggcatctg caagcagtaa
     6421 gggaaccaac cagatcagac aggccgtgga cgagctggct gcaaccgctg catcactggc
     6481 agagaatgta cagactgtta attccagaat gttggagatg ggggacaatg ttaatgcgat
     6541 ccattcggag acagtaacat tgaatgagaa ttccaataaa atggatcgtg ccaatcgtaa
     6601 tgcttcagaa tccatgaatc tggtgctgga aagctcgcat acctcttcgg acattattac
     6661 tgagatgatc gtacaggtaa aggcaaccaa tgaggccatt gcatccatta gtaaagcagt
     6721 ggaactgatt tctgatatta ccgcacagac gaatctattg tcattgaatg cttccattga
     6781 agcagcgagg gcaggacagg caggcaaagg cttcgctgtg gtcgctacag agatcaaaca
     6841 gctcgccgaa cagagcagcc agagtgcgga tacgatcaag aaaattgctg atgatattct
     6901 cgaaaaatct aataaatctg tagagttaac tgaacggatg cgtgcactgg cagaaaaaga
     6961 gcaggcagac atcggaggtg ccaagaatgg ttttgatgct ctgagccaga ttattgaggc
     7021 taatgttacg acagcgggaa ttattgcgga aaaaacgaag aatctggagg aattgaaaca
     7081 gactattata aatagtataa cagaactgag tgcgatcagt gaagagaatg cagccagcaa
     7141 cgaggaagta acggcaaacg tatctgatat cgcagaagca gttaacaaaa tttcggatga
     7201 tacaggaaca attaaaaagg tatccacgga tctggaagag ttgatgcagt attttaagta
     7261 gtttgagata tttggagaca ttcgtggacg ggtgctgctt tatgtgctat tatgcagaat
     7321 gtcatcagaa aacagtaatt gtaggaagta acagggatta taatgaaaaa aagaatgttc
     7381 atggcactgg cggtcgctct caccggagtc agtatggtgg tggcagcagg aaacggagaa
     7441 ctgaaaatac agagtgaaac ctcggtacag aaagtacatg cggcagtaat acatcagatc
     7501 ggtgtcgagc cgatcccggc taccggacag attaccatag aggatctgct ggcggtacag
     7561 ggtggcgaga cggaagatcc cagagcactg gaaggggtgt cggatccgga ggatgttccg
     7621 gtagacgaag cttccgtaga ggatacgaac gcagatgagt atgcatcgtt agcgatcgcc
     7681 aatgtgacga attacgtcaa tgtccggaca gaaccgaaca cggacagtac cgtagtgggc
     7741 aaaatctatg acggagcggt ggcacagatt ctggatgtgg caggagagga tcaggactgg
     7801 tttcggatca tctccggtaa tgtggagggc tatatcaagg cagagttttt tatttacgga
     7861 gatgcagcag ctgaggttgt agacaattat gtgaccagat atgctgtggt tatcgcagac
     7921 cgtctgaatg tgagacagga tcctgccaca gatgcaaaaa gaattggtta cattgacaat
     7981 ggagaaaagg taaaaattct ggagaatgac ggagactggc tgaaggtaca gtacaccgac
     8041 agcaaggaag gctatgtctc tgcggaatat gttacgatct cggaagaatt tatctatgcg
     8101 aagacaatag aagaggaaca ggcggaaatt gccgcacaga aggcattgca ggcaagagaa
     8161 cagactgccg agcaggatgc cccggaggtg atcacgaaca tcacacttcc cgcaactact
     8221 tatacctcca atgcggaact cagacaggga atcattgagt atgccatgca gtatctgggc
     8281 aaccggtatg tacacggagg ttccagtctg tcaggaggta cagattgttc gggatttacc
     8341 tgctttatct atgcggagtt cggatattct atcagccgta caccgggagg acagtattcc
     8401 ggtgccggac gcagtatcag ttctgaagaa cttcagccag gagatattgt atgttatagt
     8461 tccaatggag ggaagagctg tacccatgta ggactgtata tcggtgacgg acagatcatc
     8521 cattccgcca attcacggaa aggtgtcatt atcagtgctg tggactacag tcccatcatc
     8581 ggtatgagaa acgttattga ttaatagaat cggttttgaa attatggagc gcctgatctt
     8641 tggtgagtac gaaatcaccg aggatctcgt cgctccattt ttgcaggtct tccagatgaa
     8701 ggaccacacc ggcataattg cggatgccgt gggaggccgc cagctcttcg gtatattctt
     8761 ccagcagata gacattttca aaccgtaccg gtccggaggt gtactgacag accagcggga
     8821 tcacaccggt ggtcttccgc tcgatggaga caccgtcctc tgcaacgtga aaagtcagcc
     8881 aggccatacc ctctaacatg ctgagagcat ttttctgggt ggacacataa ttgcccagcg
     8941 aataatagca cagagactca ttgccgtttt ctgcggtgat ccattccaca ggctctacca
     9001 catggggatg ggttccgata atgacatcgg caccggcttc tgtcatctcc agtgcccatt
     9061 tttcctgata gcttgagatt ttggaggaat actcagtccc ccagtgagga cagacgatga
     9121 cgatgtctgc cagttctttt gcccgggaga tatcctctaa gacctgcggg ttcagcgtgg
     9181 tgaagtccat ctgtccgttt ttctcattta cggcacacag aagattcaga tgccccagca
     9241 gatctaaggg gagtgtctcg gtattggcac cgtaggtata atttaagatg gcgaaagtga
     9301 catcatcgat ggtaagtaac ggaatgtcat ctgcctgtga agcgtcttcg tgaataccgg
     9361 ttaccagtac ctccgggtgc ttgtttttcc agaaatccgc acagtacaac aggccgtcca
     9421 gcttctgatc cgcggtatga ttggatgcct gcagaaccac gttgaatccg gcatccgcaa
     9481 tcgcatcccc aacttcggta ggagaattaa aatagggaaa accggaaaat ccccgggaat
     9541 tgccgcccat gacagtttcc tgattgatga tccggatgtc tgccgcgttt aagaagggtg
     9601 aaatcccctg aaacagcatg gagaaatcat aagttccgtc ctcctgtttt ccactggcca
     9661 cgattcccag atgcatcagg ttgtccccga cagccatgag cgtaatgtca tgctccggga
     9721 agggagtggg ggtcggctcc ggagtaggga cgggaaccgg tgtggcagaa ggcggtgcag
     9781 gggtctgtgg aacgacaaca acatcgctgc ctgcagcagt ttcttccaga aagtaatagc
     9841 cgcccatgct cgccagtaac agtaacggaa cgagaaccaa cagcagatag aaacgtgaaa
     9901 tcggtttttt catatattat tcttttgttc tcctttatta tctcttacgg aattccggtt
     9961 acaaggtctg tttacaatca aaagtaacaa tagtattata gcattgattt tatcttttcg
    10021 caaaaagtaa aaacaaatta ctgttttcct attgacatag tatgaaatag gagatataat
    10081 cctacccata agttgattcg atacttaaat gagacatcga agaggaggaa aaggattatg
    10141 aagaagtttg agtggaagag cgttttggca ctggcattaa caggagcatt ggcactgggg
    10201 ctgaccgcat gcggcagcag cgataacggc gcagcagcta ccggcagcaa cggtgctgga
    10261 agcaaggctg tataccgcac tctggatgag atcaaggaaa gcggtaccat caatatcggt
    10321 gtattttccg ataagaatcc gtttggttat gtggatgaga acggcgagta tcagggatat
    10381 gatgtatatt atgcaagaag actcggtgag gatctgggtg tcgatgtgaa cttcgtatct
    10441 accgaggcgg ccaaccgtat cgagtatctg cagaccggta aggtagacgt gatcctggct
    10501 aacttcaccg taactcccga gagagcagag gaagttgatt tcgctgctcc ttatatgaat
    10561 gtagcacttg gcgtagtatc caaggatgac aatgtaatca ccactctgga taactggaat
    10621 gcagatgatt ctattattgt gatctccgga accaccgcag agacctatct gattgctaat
    10681 tatccggaca ttcctttaca gaagttcgat tcctatgcaa ccgctaagga agcttttgaa
    10741 aacggtactt ccgtagcatg ggcaaacgac aacactgagg tcatcgcatt tgctctgcag
    10801 aatgaaggct ataccgtagg tattccttcc ctgggcagcg ctgataccat cgctcccgca
    10861 gtatctaagg gcaatactac tctgcttgac tggatcaacg atgagatcgc aagccttggt
    10921 gaagagaact tcttccatgc agattacgag gcaactctgg cagataccta tggtctggat
    10981 tacgaggaca gcctggtagt agaaggcggc aaggtaaccg cacaataagt tcggatcgga
    11041 atagaatgat agagagggga ctgcggggca cgtatgttgc cgc
//
LOCUS       DAWEJW010000093        10713 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2138_length_10713_cov_63.243854, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000093 DAWEJW010000000
VERSION     DAWEJW010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2138_length_10713_cov_63.243854"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2457 a         2964 c         2333 g         2959 t
ORIGIN      
        1 tcacccacct atgagcaagc tcatgtgggc ttagaccttt tgaccctggc atcatataat
       61 aaaaatacga aagagaacag gtgtggggaa tctccctcac ttgttctctt tcgtatttta
      121 tgttctgtat tcgtaatata cttagaaatc tttttttctg tcgttttcca gattggcaaa
      181 cttggtgtat tccggcagcc atgccagctg tacggttccg atgggaccgt ttctctgttt
      241 ggctatgatg atctccgaaa tacctttatc cggcgaatcg tgattataat aatcatcacg
      301 gtagatgaac attaccacgt cggcgtcctg ctcgatggct ccggactcac gcaggtcaga
      361 caacataggg cgatgatccg gtctctgctc tacagcccga ctcaactgag acaatgccag
      421 caccggtacg gacagctctc tggcgactgc tttcagggat ctcgaaatat cggaaatctc
      481 ctgctgtctg gaatcgctgc ttcttccgcc acttcccgac atcaactgta agtagtcgat
      541 gatgatcatg gacagaccgt tttcctgctt cattcttctg catttggaac gaagctcggc
      601 aatgctgata ccgggggtat cgtcgatcat cagcttggac ttgccgatga caccggcact
      661 ctcgatcagt cgttcccatt cctggtcatt cagctggccg gtacggagct tctgggcatc
      721 cacgctggat tccagggaaa acatacgatt gatcagctgc tccttactca tttccagact
      781 gaatatggct accggcagat tctgcttaaa tgctacatgc tgcgcgatat tcaatacaaa
      841 cgccgtctta cccatggaag gacgggctgc gatcagcacc aggtccgagg gctgcattcc
      901 ggcggtacga tagtccagat caatgaatcc ggtggcaata cctgtgacgg ttccgtgcag
      961 tttggaagcc ttctcgatct tgtccatggc gttcatgact acggtacgaa tcggtacgaa
     1021 ttccttgacg tttttcttct gtaccacttc attgatcttg ctctcggtac tctccagaat
     1081 cgtatccagg ctctccttgc cactataaca ggtattggca atctcttcat tggtacggat
     1141 cagacgccgg agtgtcgcct tctccgccac gattccagca taatacttga tattggcgga
     1201 agtaggtact gctgcaatca gattcgtcag gaaatccagt ccactgacct cgggaggcac
     1261 atcctccttg gatcgcaacc gatcctgcaa agtgacaaag tcaatgggct ttccttcatt
     1321 gttcaactct acgatggtgt caaataccac accgtactgc ctgttgtaga aatcctctcc
     1381 ggagatctgc tctgtggcgg ccgcaatggc ttccgcgtca atgaacatag agccgatgac
     1441 cgactgctcc gcctctaaac tgtgaggcat tattcttttg attacatttt catccattgc
     1501 gggcatgggg tcgtctacgc ctccgtaact ttcaccgtca gcttcgctgc cacctgggga
     1561 tgaagcttca ctgttacctc ataggagccg aagttcttca gactctccgg cagctggatt
     1621 ttcttcttgt caatctcgat accatgctgt tccttgcagg cagttgcaat ctctttggag
     1681 gatacagaac cgaaggttct tccgccctct cctgccttga tcttgactac gacctgctta
     1741 gtctccagct ctgttgccag cgcctttgcc gcatccagat tctcctgtgc caccttctct
     1801 tcgtttgcct tatggagctt caggtcgttc agattcttgg agcttgcctc cacaccgatc
     1861 ttcttcggta aaatatagtt tcttgcatat ccttcgctgg cctcaatgat gtcacctttt
     1921 tttcccagct ttttttcatc ctgaagtaaa ataattttca taggatcaac ctgcctttcc
     1981 tgaatcttct gttttatata gtaaaacttt tcaaacggaa tcccttagat ctcaccggat
     2041 tccagcatgt cgtcgatggt gtgcttcaat acgccaatgg cctctaccag tcccacgcct
     2101 tccatctggc aggctgcggt attcatgtgg cctccaccgc ccatacgctc catgatgatc
     2161 tgtacattga cctcatcaat ggatctggcg ctgacataga tcttgccctg ataatcggta
     2221 agtacaaaac ttgccttaat gcccttgata ttcagcagtt cgttggctgc ctgcgcacca
     2281 atgatggtag ggctgggcag atcctctgcg gtacacagag agattgcaaa gaactgcttg
     2341 tagatctctg cctgactgac agcatccgcc ttggccttgt attctgctgc atcctcacgg
     2401 aacagttttc tcacacgggt cacatccgca ccgtttctgc gcaggaatgc ggcagcctcg
     2461 aaggtgcgga cacctgtctt ggtcataaag ttattggtat cgatcatgat accggaatac
     2521 atgcaatccg cctcttccgt acggatcttg atattgtcgt aggtatactg caagatctcg
     2581 gataccatct cgcaggcgga ggatgcataa ggctccacat aagacaatgt ggcattctca
     2641 atggtctccg taccctgtct gtggtggtcc agtactacga cggatttgca gaagcgaagc
     2701 agatccgggc actccgtgat ggagggttta ttgacatcca ccaccaccag aaccgtattg
     2761 ctgcccgcca tatcaatggc ctgctggctg gtaatgatca tatcctcgtc atattccggg
     2821 ttgttcttaa acagttctac cagaggctgt accgaagtag tagcctcatt caatacaatg
     2881 tgagcctttc tgccgagggt ctgggcgatc cggtaaatac ccaccgcaga accgaagctg
     2941 tccacatccg gcatccggtg tcccatgaca atgacacgat ccttgccggt gatgatttcc
     3001 cgcagggcat gagcttttac tcttgccttg acacgggtat tcttctctac ctgctggctc
     3061 ttgccgccgt agtaggtaat gctctccggc gtcttgatga ccgcctggtc accgccgcgt
     3121 cccagcgcca gatcaatggc ggtacgggca aactcgtagt tctgcgcata tgtcagtcca
     3181 tccagaccga taccgatact gatggtcacc gccatctcgt tgccgatgtt caccgtcttg
     3241 acctcttcca gcagatcaaa acggttctcc tgcagctgtg ccactgcttt tttccgcatg
     3301 attaccagat acttgtcctt ctccagcttc ttgctgatac catccagtgc cgcaatgtat
     3361 ttatttacct tacggtcgat caaagcaatc agcagggatc tgcggacttc ctcgacgctc
     3421 tccagggctt cctcgtagtt gtccagatag atcatgccta cggtcaggct ctggtcatcc
     3481 acttcctgca atgcgatatg cagtgccgtc tcatcgaaga gatacacagc aatcaggtaa
     3541 ccgttatagc cttccgcatc gatcatatcg ctgtgttccg ccatctcctt cagagaaatc
     3601 ttgcggaatt tggccacata ctcacagccc tcgtattcca gttcatactg tgcctcgtcc
     3661 acaccagatt cgtccggaag cctgtcccgg gtaatggtag gaaacaagga agtgatggat
     3721 ttactgtagc ccttgggctg atgcaccaca ttctcaaaag caatgttggt ccagatcacc
     3781 ttgccggaat cgtccagaag ggcgtatggc aattccagat cccgcaacag ttttcgctgg
     3841 atctgaccat attccgtggc aaagctgatc agctcgttca tgatgatcgg cttgttgtag
     3901 aaatacagcg tcagggtaat tgcaaaataa aaaatcgtaa atccgaaggt aagcagtcct
     3961 gcacggaaat ccaacatgaa taccgccgca tcgatagcca gcagtaacag gcccagatat
     4021 agggtaaact ggatatatgt acggatcctg cctttcagtc tgattcgttt tttcatgaaa
     4081 gaacacctct caatatttgc gttcgacaac ttctattata acaaataata acccgctccg
     4141 ccaccatatt attttgacga atagacaata ttcctctgtc aaatttgtgc tttttgtcag
     4201 cttttccgga cggagagccg ttctcttact tacacagttc ccagaaagcc actgcgctgg
     4261 cggccgccac gttcagagaa tccacaccat gggccatggg aatgcacacc gtatcgtcac
     4321 agtccgcaat ggtatccgct gccagtcctt ccccttccgt tcccagaata atggcaagct
     4381 tttcgacatt atgcagtttc tcatcatccg gccgcaagct gtcttctttt aaagccatgg
     4441 ctacggtatg ataccccatc ttccgcagct gttccatggt atgctccggc cagtcctttg
     4501 tctccaggat cgtccacggc acctggaata cggttcccat gctgactctt gctgcccgac
     4561 ggtacaacgg gtcactgcag cctcccgtca acaatactgc atcgattccc aacgctgccg
     4621 cagaacggaa aattgcgcct acatttgtgg gatttacaac attttccaga acagcaatgc
     4681 gtctggcatt tccgcacaag tcttccatct tcggcaatgc tcttctgcgc atggcacaga
     4741 gcattcctct cgccagttta aaaccggaaa tctccgacag cactgcattg gatgccacat
     4801 atacgggaat ttccccagac acctctgcaa tccgccccag gatttccctg cccttctccg
     4861 aatccgcttc ccgctgttcc atcagaagtg acagcggctc atatcccgct gccagtgccc
     4921 gcagaatcac attggggctc tccgcaatga acagcccccg ctctggctca taataatgaa
     4981 aaagttgtac ctctctgcgt tcattgtaga tctccagttc cgctgtatgc agatcagtaa
     5041 ttggtattaa tctatgctta tctgtactca tgcttttgcg ataccttttc tccaataatt
     5101 ttctcttttt attataatgg agcatccggc ccccgtcaaa cgcaactgtt accctgtact
     5161 cggctcatgg tatacacaaa aagaccagcc acaaagggct ggtctctcat cttagtctga
     5221 attattcagc tacgtaaggc ataagtgcta cgtgacgtgc tctcttgata gcaacggtca
     5281 gtgctctctg atgctttgca caagtaccgg taatacgacg aggaaggatc ttacctctct
     5341 cagatacata cttcttcagc ttagctgtat ccttgtaatc gattacgtta tctttaccac
     5401 agaatacgca aactttcttt cttctgcgca taccgccttt ttttctcatc ggagaatcgg
     5461 tcttctcagt cttattaaaa gccattatta ttacctccta tcggaatctg tcagggcaga
     5521 ggcgggtgtc agctccgctt gtcactgaaa cttagttaaa gggtaattct tcatcgatgc
     5581 cgtcagggat gttcataaag ccatcaccgg ctccggaagg agcgggggca ctattaccac
     5641 caccaaagcc agcgttctgg tatccaccgt cattattgcc ggcagatgcg ttcttactct
     5701 ctgcgaactc tacgttctca gccactacat cagtagtata cacacgctgt ccatccttat
     5761 tggtatagct tccggtctgg atacgtcctt ccacaacgaa ctttgttccc ttgtggccgt
     5821 atctctcaat aaactcaccg gttctgccaa acgctacaca gttaataaaa tcagcatccg
     5881 gctcaccgtc acgcttaaat cttctatcta ctgctatgga aaaacgagca atcgctgttg
     5941 cggtagctcc ctgagaatat cttacctcag gatcccttgt taaacgtccc ataagaatta
     6001 ctttattcat atttcgcttc tcgctttctg tttatgcctc gtcctgtcta acgcataagt
     6061 atctgatcac gttgtccatg atacgaacac ggctctcgat ctctgcagga acagtgctct
     6121 cagcttcaaa tctgatgaag tagtagaaag cttctttcat cttctgaact tcgtaagcaa
     6181 gcttcttctt gccccactcg tccacttctg tgatgttgcc gccgaatctc tcaaccagtg
     6241 ctttgcactt gtcaacaacg gctgctctct catcatcctc gatcttagcg ctaacaacaa
     6301 cggctaattc gtacttgttc atgttagtta cctccttttg gtctctggcc catattttat
     6361 atatgagcaa ggaataaagt gtctcacgaa agaacatgat accatatacc cgtgcatttt
     6421 tcaagctttt tcaatgattt ctttcacatt tttttctaac agttttcttg cgatcatgat
     6481 ctggtgtcca cagcccagac atttcagccg gaaatctgct cccacacgga gtacttccca
     6541 ttctctgctg ccgcagggat gctgtttttt cagtttcaat acatctccca cattaatatc
     6601 cataacattt tcctcttctt ttcctgcctg tatcgccact cctccgaaaa catgtcgcga
     6661 cagaggcctg tgattctgta ttataaatgg ttcgccataa agtccttcac taccccgaaa
     6721 gcctcccgca gcaggttatt cggtacggta atgggataag ttcccgcgga cagtccttct
     6781 acatgggcat agccctgttt tctcgcaata gccaacgccc ggaacatatg aaaattgttg
     6841 gtaataacca caacactgtc ctctgatttg tccagcagtt ctccgctgaa gatcagattc
     6901 tgcgtggtat tcgtggaagc atcctccatg aggatccgct ccggatcgat cccggcggtt
     6961 tccagatatc cttgcattcc ctcggcttcg ctgatgggtt cattgtatcc ctgtccaccg
     7021 gacacgatga ctttggtctc cggattggca ttcagatatt ctatggcttt atccagccgt
     7081 ttctgtagca cataactggg gccggaagtc ttccactggg ctcccagtat gatcacataa
     7141 tccgcacctt cctgcgccgt ggcatggaac tgactgacga tcatcccttc tataataaga
     7201 agcaatacca tccctgccat cacaagactc cggaatgtaa tatgcagcca tcgggggatc
     7261 cagtaatata cggttctgtg ggtcagcacc catgcccatg cggcacaaaa agcaccaatc
     7321 acaccccaga ccaggaaaaa gtagctgccg aaggcaacaa aaagaccgat ccccacacag
     7381 tacagcagac acagcaccgc cagaacatat aacgcccttg ctccgtaatg tttttctccc
     7441 acacctctgc ctctgggatc cagatagatc acttttttac ttttttccct cggaatcaag
     7501 tggttccttt tcatactgcc tccattcttt cctacgatct attgtagcat atctcctacg
     7561 gcagtctcaa taccacatat tttagggttt tcttaatttt ttcgaaaatt ttctttaccg
     7621 gacactgatt gacttatcac tcacctaaca ctatgatgta attaacatac tacttctgaa
     7681 aaaagaaacg gatggaaacc ttatgcatac cacattatcc aaaaaagatt tttcccgata
     7741 tctgcccttc ctcctgctgg tgatgaccgt tttccgcgtc ctcgccggac tgaggattcc
     7801 ttatatgatc ctggcaaatc agcgctatga tgaccggctg ctgttcgaga atgcttacga
     7861 tctgctgagc ggcgtgtggc ttggcagcta cgattcttat acactggcaa aaggaatcgg
     7921 ctatccgctg tttctggtac tggcgaaaaa gctgtgcctt ccctactctg ttctgctgtc
     7981 gctgttgcag gcggccggtg catggctgtt tgtccgcgca gtgtctgtga gatggcagaa
     8041 tccctatggg caggcaattc tctatctgct cctgttgttt tcccccatca gcctcacgct
     8101 gttggtcacg cagcggctgt accgcatggc aatcgttcca ggaatggtcc tggtggtatt
     8161 ttccagcatg atcggactga ctctgcgcaa agagctcccc ttgaaaaaac agctgccctg
     8221 ggcattcctg accggactga ctcttgcctt tttctggcag atccgggagg attccgtctg
     8281 gatcctgccg tttatcgccg ttatgacggt atggaacgtg ggttatgtaa ttctggtcct
     8341 gcataagaaa ttaaacacaa aggcacttct gcttcactgc ctgaccatgt tattgccact
     8401 ccttctcctg ttcggcgcca acaccggtgt ctccgtcgtc aaccggatcc actacggtgt
     8461 tttcctgaac aacgaccgca cggagggaaa ttttgcagag ctgatgtccc tgttgtatca
     8521 tctggacagc aatacgagaa ccaatcccga catctggatc tcccgggata ctattgtcag
     8581 agcagaagca gccagtccca ctctgcagca gattcagcca ctattggaca gttataccga
     8641 agactgggcc acaagggatg gtgagattcc cggcgatcac ttcagctggg tactgcggga
     8701 tgcggtacag gactcgggaa ttgccccgaa tgccgtcagt gcacagacct tttatggcaa
     8761 cgtcctctcc gaactgcggg cagccgtagc cagtggcgag ctgaccgaaa agacggacgg
     8821 tgccctctat ttttcctccc aatcccgtgg cgtactgccg gaagaaattc ccggtatcct
     8881 cagtgatact ttgcagaata tctggaaaat tgcaggatac accaattgtg ctttgagcag
     8941 ttccgccaaa agtgccggcc ggctgtcgga tatccgtcgt atggaatcct ttgcctcctg
     9001 cctgaccgtg taccccacac tgtcacagtt tcaggctgcc gattatgatt ccattgagga
     9061 tgagagctta tatgatttca atgagatcta cagttccggt atggtatctc ttctgaataa
     9121 gagcaacgct atctatcagt tgttgggaca gcccatgttc ctgctggcac tgcttgccct
     9181 ttgtgtgctg accgtccgcg tgatccatgg acttttccat ggggacacaa aagatctcga
     9241 actgtggatc ctgacctgtg ggattctgct cagtgccgtt ctgctgcgtt tcggctgtgg
     9301 cctgttcacc gcatggtttt ccgaagacat gcaaaagttc atcaacagct tctactcctg
     9361 tggcgtctac attctgctgc agatgttcaa atatctggcg gttattacta ctgccgccag
     9421 cctgcaaccg atacggaaac aatattttca aaattttcgt aattctcata aggaaaagga
     9481 ggaataactt atggaaaatt tgaacaaaca gattcaggaa ttgcaaaaga tcattgatac
     9541 ctacggcaat atcgtatttt tcggtggtgc cggtgtctcc accgagagtg gaatcccgga
     9601 tttccgaagc gtggacggac tgtatcacca acagtacgat tacccgccgg agacgatttt
     9661 aagtcacacc ttttatcgtt cccacacgga ggaattcttc cgtttttacc ggaataagat
     9721 gctcttcccc aatgcacagc ccaatgccgc ccataaaaaa ctggcagaac tggaagctgc
     9781 cgggaaagtc cgggccgtga tcacccagaa tatcgacgga ctgcatcagg ctgccggaag
     9841 caggaccgta ctggagctcc acggctccgt gcttcgcaat tactgtgaaa aatgctatca
     9901 gttctatgga atcgacacga tcttaaacag caccggtatt ccccgctgtg aaaaatgcgg
     9961 tggtatcatc aagccggatg tggttctgta cgaagaggga ctggatgaga agacgttaaa
    10021 tgccgccgtc agatacatcc acgaagcaga tgtcctgatc atcggcggca cttctctggc
    10081 tgtctatccc gcagccgggt taattgatta ttttcagggt gataagctgg tggtcatcaa
    10141 caaaagtgcg acccccagag acagccacgc agatctcctg gttcagggcc cgatcgggga
    10201 agtatttcat tcgatccaga atccgtaagt gatatcctgt tctccgctga tatgataagc
    10261 atcttccaca tcagcgcccc agctgttgat accacccacg cccctcactg ctcccaggat
    10321 ggataccacc gtcctgcggg caggaggtag ttcttcgtgg tgcatggcat tttccagttc
    10381 ctgtgccgta taaggcaggc aggaaaatgc gaagtatttt ccctcctccg cggttatttt
    10441 taaacggctg cttctttctc cacggtctgt attatccagg gaagtcttcc taacgatctc
    10501 cagccatttg gtctgcatat gcatcccaca gtcctgaggc accatatagg gtgtcaccgg
    10561 caatccctgt acttcataca caccgggaat tcctcctgcc atacggtccg gataggtctc
    10621 tccggacaat ccttcatagg tatacctctc cgctgcggtg ggcatcagga accgcattcc
    10681 gaataccgga agctccggca gaccctgttt tcc
//
LOCUS       DAWEJW010000094        10620 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2163_length_10620_cov_69.530809, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000094 DAWEJW010000000
VERSION     DAWEJW010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2163_length_10620_cov_69.530809"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3129..3228
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7184..7283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2873 a         2227 c         2997 g         2323 t
ORIGIN      
        1 ttgtaacagc aatgacactg aattgaggtt ttcaatgaat atagtagtag acggatataa
       61 tatatgtgta aaaacaaccg gtaccggcga taagacagtc gtgatcttac agggctgggg
      121 tacggatctg ggagtgtatg attccgtagc cggtgtgata gacggcagca ggtaccgtgt
      181 gatccagttc gatttcccgg gctttggcgg cagtgatgaa ccgaaggaac cctgggatgt
      241 agacggattt gcggattttt tctgcagatt tatggaagta atgcaggtaa agcaggccac
      301 cctcatcggg cattcctacg gtggccgcgt gatcatcaag ctggcagcca gagagagcat
      361 cccttttgag atcacaaaca ttatcctgat cgacagcgca ggtgtcctgc cgatgcgtac
      421 tactgcacag aaatggaaga tccgcaagta caagatcctg aagaaattcc tgaacatgaa
      481 gctgatctat gctatgttcc cggaggtgat cgatgactgg aggagccgac agggatcggc
      541 agactaccgc aatgcgacac ccatgatgcg tcagtgtatg gtgaaggcgg tgaatgagga
      601 tctgacggag ctgcttccga agatccgtca ggaagtgctg ctgatctggg gagatcagga
      661 tacggcaact cctatccggg atgcacatat tatggaagag aagataccca actgcggtct
      721 ggcagtgatc ccgggaaccg gtcatttttc tttccttgag aaaccggcac agttccgggg
      781 aattatggag gcatatttgc aatgatcata cgtgtgctga tagcggtcgt gcttgctgtt
      841 ccggcatatt tttataccat gcggtataac attcatatgt tccagcagaa cggctataag
      901 aataaagaac acattcactg gctgaagaag aaattccggc tgcaatggat tctctatttt
      961 ggtctggcac tgggcattgt aacctgtgtg tttccgtacc ttgcccttga aatcttcgag
     1021 tatctggtac tgtttgtgat catcgtggta tatcgtgcca tgaagcggat gaatacgaag
     1081 aaaaagctgg tgtatactgc ccgggtcaag agattgctca ctacggagct gatccttacg
     1141 gctgcggtat tcgctctggt gtgtggattg ggcggaatga agcggatccc gggactgtgc
     1201 ctgatcctgg tatctgtgaa tctgtttctg gatattgtgg ctaatgtcat caatcatccc
     1261 atggaggcgg ggatcaatca gcattatatc aatgatgctg tgagaaagct gaaaagcgtc
     1321 cagggactta ccgtcatcgg tgtgaccggc agctacggca agaccagtgt aaagttctat
     1381 ttgcagacat tattgcagga aaaatataat gtactggtga caccggagag tttcaatacg
     1441 cctatgggtg tggtgcggac catccgcggt tccctgaagc ctaccacaga gatttttgtc
     1501 tgtgagatgg gtgcccgcca tgtgggcgat atcaaggaga tctgcgatat ggtgcatccc
     1561 gatcacggtg tgatcacatc tatcggaccg cagcatttag agaccttttt taacatggag
     1621 aatattcaga agacgaagtt cgagctggcg gatgccctgc cggaaggcgg catgctgttc
     1681 ttgaacggtg acaacgatta tattcagcag caggcagctt ctcccgcgta tgatcagacc
     1741 ccggagaaga tattctatta ttccgaaacg gaagggacag gatactgtgc caaagatgtg
     1801 aaggtatccc agttaggtac ggagttcacc gtagtgaccc cggacggcga gagcgagaga
     1861 ttccagatgc gtctcatcgg tgcccacaat gtcatcaatg tggtgggggc tatcgcggtc
     1921 gctcataaaa tgggtatgac gctgcaggaa ctgcggatcc cggtacgccg gatcgagccg
     1981 gtaccccatc gtatgcagat gcgggagcac ggtctggtga cgatcatcga tgatgcttac
     2041 aattccaacc ctgtaggttc cagggcagcg gtcgagacgc tggctatgtt tgacggtatc
     2101 cggatcctga tcacgccggg catggtggag ctgggtgata aggaagcgga atacaatcat
     2161 aaattcggta actacgcagc ggattgctgc gattacatcc tgttagtggg cagacgacat
     2221 accgagccca tcagggaggg cgtgttggag aagggatttc cggaagagaa atgcctggta
     2281 ttcgacaagc tggaggaagc tgtatcttac gcctatgcca ttaaagggca gggacataag
     2341 tatattttat tagagaatga cctgacggat aactattaga agattcagag gcaattttgt
     2401 gaaagaggtt ccggatagtt ggcagagagt aggaaaattc aggagggaaa ataaatgaag
     2461 acaagagtag caatgctgtt tggcggcaag agtgtagaac acgaagtatc agtgatctcc
     2521 ggtatccagg ctgtcatgaa catggatact gacaaatacg cagtaatccc ggtctatatg
     2581 accaagcgca atgaaatgta catcggtgag gagatcggca agatcgaatc ctataaaaat
     2641 atcgatgaac tgttgaaaaa gtcccagaga gtcatcatga ccaacgagga cggcaaggta
     2701 ttcctgactc cgttcccggt gaagatgttc ggcggaaaga aagccgtgga gatcgatgtg
     2761 gctttcccgg tagtgcatgg taccaacgta gaggacggcg ctttccaggg ttacctgaag
     2821 accatgggca tccccttcgt gggctgtgat gtgaccgcat ccgccattgg tatggacaaa
     2881 tacatcatga aagctgtatt aaaggagtgt gatgttccgg tactggatgc gcagctgtat
     2941 actctgtcgg attatgcgca gatagagatt ctgctcgaca aagtggaaaa aggactggga
     3001 tatccggtga tcgtaaaacc tgtcaactta ggctccagcg taggtatcag cgtggcaaag
     3061 aaccgcgtgg aactgaccca ttccgtggac gatgctttca agtatgccac taaggtactg
     3121 gtggagcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg tatgatccag
     3241 ctgtctttga agagagcccg caccgaggag agactgacct ttacctttga caccaatatc
     3301 ctgaattccg caagctttgg cggttctaag ggtggcaaat tgtaatagcc ggtttatgga
     3361 ttttatgatt atttaatata ttttttagca ctcgggactt gcgagtgcta agtagtagtg
     3421 ataatataat ctctgtaaga ggaacaagcg actgcaacag cagacaattg tttgaaaaga
     3481 gatgtgaagt ggtcagccgc gcaggaaact gtgtggctga tttttacatg gaaaacgtct
     3541 tggttgtgtg gctcaccggt tgggcagaat ggagggaata tgacgaagac aacaaaaaga
     3601 gattattatg aagtactggg tgtcagcaag acagcggacg ccgcagcgat aaaaaaagca
     3661 taccggaaac tggcgaaaaa ataccatccg gataccaatg caggagaccc tgtggcagag
     3721 gagaagctga aggaagtcaa cgaagcttac gatgtgctgg gggatgaaaa aaagaagaaa
     3781 ctatatgata cttacggttt tgctgctttc caggagggat ttaatgagga ggcagccaga
     3841 cagtatcagg agcagtttaa gaacggaggc ggcttcggca ccgatggatt cggcggcttt
     3901 ggcaacggca cgttccatta cagcaccgga ggaagcggtt tcggtaccgg tgattttggc
     3961 ggcttcggcg gggatgagga tattttcgac aatctgttcg gtcagtattt caagggacag
     4021 aaaggctccg gacggggcag ccgggcgaac taccgcagta aaggtcagga tgtggaatcc
     4081 ggcatcacca tcagctttga cgaagcaatg catggctgcg ataagacctt taccttaaga
     4141 gatccggcta ccggcaagtc cgaatccgtg caggtgcata ttccggcagg tatcgacacc
     4201 ggtaaaagta tccgcctgaa aggtaagggt ggtgaaggtt actccggcgg tgagagcggt
     4261 gacctgtatc tgaaggtgac ggtacaggag aagccgggct gggagcgtag aggacaggat
     4321 ctgtatacca cggtcaatat cccctatact accgcagccc tgggcggcga gatccgtgta
     4381 cccacgttgt acggtgatgt catgtgcaaa gtgaaggaag gtacgcagtc cggaagtaag
     4441 atccgtctga aaggcaaggg cgtggtatcc atgaaggata aaaatgccca cggcgatcag
     4501 tatgtcgtga tccagattca ggtgcctaag aatctgaccc cggcagcaaa agaaaaatta
     4561 caggaataca agaaggtcag tggattttga tccactggcc ttttgtgttt ttgataaatg
     4621 ataaaatttt tcattaaacg aaaaaagctg gaaataggaa aaataggtgt ttataatatt
     4681 cattcataaa tagaataaac caaagaattc gcacaggagg aaatgaatta tgttgaaatg
     4741 tttaaaaaag attgataaag caaaaaccgg tattttcgca gcaggcgttc tgttcggaac
     4801 cgcaggcatc aaagtactgt ccagcaagga tgcgaagaag ctgtacacca actgcaccgc
     4861 agcagttctg cgcgcaaaag agtgtgtcat gaagactgcg accaccatcc aggagaacgc
     4921 agaggatatc tacgcagaag ctcagcagat caacgaggac agagcagcaa aggcagctca
     4981 ggagtttgac ggcgaggttg aggcagaagc agcagagact gcagcagacg ctgaataatc
     5041 catcgaatct tacaacacag aataaatgac acctgcatgc ttcggcatcg caggtgtttt
     5101 catgaaagca ggcataaatt atgagatttg tgatcaaaca tgagataaag gggagactca
     5161 gggttcacat tctgcagagc cgtatgagct ttgcacaggc agatacactg caatattatt
     5221 tggacggaca gagtaatatt gtcagtgcca aaattcagga gcgtaccctg gatgtcacgg
     5281 tagtctatac cggttccagg ggggaagcac tgaagacact ggagaatttt acatatcagg
     5341 gtacagaagt gccggagaac tatcttgcta attccggcag agagatgaac cgggaatata
     5401 aggatcagct gatcaataaa gtggtaatgc attatgggat caggctgttt ctgcccatgg
     5461 acatccgttc cgtgatcacc acagtgaagt ctttcaaata tctctggcac ggcgtgaaga
     5521 ctctggcgaa gggcaagatc gaagtaccgg tactggatgc cactgctatc ggtgtatctg
     5581 tgctgcgtgg tgattacaac acagcaggtt ccgtaatgtt cctgctcggc attggcgaga
     5641 tcctggaaga gtggacgcac aaaaagtctg tcggtgatct ggcgaggagc atgtccttaa
     5701 acattgacaa ggtatgggtt gtgagcaacg gacaggagat cctggtgccg tccacatcca
     5761 ttaagtcggg tgatctggta aggatccaca tgggcaatgt gattcctttc gacggtacag
     5821 tgacggatgg agaaggtatg gtgaatcagg catctctgac cggagaatcc gttccggtga
     5881 gaaaaattcc cggtggaact gtctatgccg gaactgtggt agaagaaggt gagctgacta
     5941 tctgtgtgaa agaaaacggc ggctccagca aatatgagaa gatcatgacc atgatagagg
     6001 aatccgagaa gctgaaatcc tctctggagg gtaaggcaga gcacctggca gataagctgg
     6061 taccctatac tttcttaggc accggtctgg tatggctgtt cacacggaat gtgaccaagg
     6121 cattatccgt gctgatggtg gatttctcct gcgcattgaa gttgtctatg ccgttagcag
     6181 tattgtctgc gatccgtgag gcaagcacct atgagatcac ggtgaagggc ggcaaatact
     6241 tagaggctat ggcagatgcc accaccattg tatttgacaa gaccggaacc ctgaccaagg
     6301 cacagcctac cgtggcggat gtggtatcct ttaacggaca acccgcagag gaactgcttc
     6361 ggatcgccgc ctgcctggag gagcatttcc cgcattccat ggcaaaggca gtggtgcggg
     6421 aagcagcccg caaggagctg gtacatgaag aactgcatac caaagtggaa tatattgtag
     6481 cgcatggaat ctcttccact ctggaaggca agaagatcat catcggcagc taccatttcg
     6541 tatttgagga tgagcaggca cagattccgg agggcagaca ggagctgttc gaccagctgc
     6601 ctgaggaata ctcccgcctg tatatggcaa ttgacggcaa gctggctgca gtgatctgca
     6661 tcgaggatcc tctcagagaa gaggctccgg aggtcatccg acagctgaag agtctcggaa
     6721 tccacaaggt agtcatgatg acaggagaca gcgatcgtat cgccggggca attgcgggta
     6781 ccgtcggcgt ggatgagtat tattccgagg tattgccgga ggataaggca agattcgtgg
     6841 agcaggagaa gcaggcaggt cataaagtca ttatgatcgg tgacggcatc aacgattccc
     6901 cggcattgtc tgcggcagat gttggtattg ccatcagcga gggcgcacag atcgccagag
     6961 agatcgccga tgtcaccatc ggtgcagaca atctgtatga agtagctacc ctgaaggaat
     7021 tgagcaactc cctgatggcg cggatccata aaaattaccg catgatcgta gggatcaaca
     7081 ccggactgat cattttaggt gtggcaggaa tcattcctcc cactacgtcc gctcttctgc
     7141 ataatacatc taccctgtgg atcagcacca agagcatgaa gaannnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnacgatag agataggagg aagtatatgt cagaacaatt
     7321 attacaggtg cgtaacttgt ccgtaagtgt agaggacaag cccatccttc atggaataga
     7381 tttagaggtt cgtcccggag agacccacgt cctcatggga cccaacggtg ccggcaagtc
     7441 cacattgggc tacaccatca tgggaagtcc ccgctatgag gtgaccgatg gtgagatcct
     7501 gttcaaagga aaagagctgt tggaggagac tgcagaccag agagcgaagg acggtatctt
     7561 tttatccttc cagaatccgc tggaggttcc cggtgtctct ctgcggaact ttatccgcaa
     7621 tgcggtggaa cagagaagtg gttcccacat caagctgtgg acatttaaaa aggaactgga
     7681 gaaagctatg gaagtcttac agatggatcc ttcctacggt gaccgtgacc tgaatgtcgg
     7741 attttccggc ggcgagaaga aaaaggcaga gattctgcag ttacttatgt tgaagccttc
     7801 actggccatt ctggatgaga cggattccgg cctggacgtg gatgcagtgc gtaccgtatc
     7861 caagggcgtg gaagagtacc agaaggacaa aaagggtgct ctgctgatca tcacccacag
     7921 caccagaatc ttagagtatc tgcatgtgga caagacccat gttctggtaa acggcaagat
     7981 cgtggcatcc ggcgatgctt ctctcgtaga cgagatcaac gagcatggtt tcgagcgttt
     8041 tctggaagca taaggaggtt gacgatctga atgagtgaac gtgaaaagac atacgtggaa
     8101 gacgtcaaca gaagcattta tgatatccgt aatgaggaga aggacgtcta tcgtattaaa
     8161 aagggactga ctcccgatat tgtggcggag atttccgaga aaaagaagga tcccgcatgg
     8221 atggcaaatt tcagattaca gtctctgcag atctataatg agatggaagt tcccgactgg
     8281 ggtccctcta ttgagggtct gaacatggaa gacatcgtaa cctatgtaaa acctaatacc
     8341 catatgagcg ccaaatggtc cgaagtgccg gaggacatca aggatacctt cgaaaaattg
     8401 gggatcccac aggcggagcg gaagtccctc gccggtgtgg gcgcccagta cgattccgag
     8461 ctggtatatc acaatgtcag ggaagaagtg gcagcccagg gcgtagtcta taccgatatg
     8521 gagagtgccc tgaatgggga atatgcggat atggtgagga agcattttat gaagctggtg
     8581 acccccagag atcacaaatt cgcagcgctg cacggtgctg tgtggtccgg cggctccttc
     8641 gtatatgttc cccccggagt gtctgtgacg attcccctgc agtcttattt tcgactgaac
     8701 gcaccgggag ccggacagtt cgagcatacc ctgatcattg tggacgaggg tgcatacctg
     8761 cattttatcg aaggctgttc cgccccgaag tacaacgtgg cgaacctcca tgccggctgt
     8821 gtggagctgt tcgtggggaa aaatgcaaaa ctgcgttact ccaccatcga gaactggtcc
     8881 aagaatatgt acaatctgaa taccaagcgg gcgttggtgg aagaaggcgg taccatagaa
     8941 tgggtatccg gttccttcgg atcccacgta tcctatctgt accccatgag cgtgctgaac
     9001 ggtaaggggg ccagagcgga attcaccggc atcacctttg cgggagccgg acagaatctg
     9061 gataccggta ccaaagttgt ccacaatgca ccggagacca cttcctatat caataccaag
     9121 tccatctcca aggatggagg tatcagtact ttccgaagtt ccgtagtggt gaagaacagt
     9181 gcggcaaaga gcaaatccgc cgtatcctgt cagtccctga tgctggatga caagtcccgc
     9241 tccgatacca tcccggccat ggatatccgt acccaggatg ccgatatcgg acacgaggcg
     9301 aagatcggcc ggatcagcga tgaagcagtg ttctatctga tgtcccgcgg tatctcagaa
     9361 gaggatgcca gagccatgat cgtcagcgga ttcgcagaca atgtatcgaa ggagctgccg
     9421 ttggaatacg cggtggagat gaacaatctg atccgtctgg agatgaaggg cagcatcggt
     9481 taatgtggca ggaggaatgg agatcagtat ggaaaaagaa tataatatga ccattaacag
     9541 actgccggcg aagacctgga actggcttcg catgaacgag acgcagttgg aaaagataga
     9601 agtacctacg gcaggagggg taaaggtaag cctgccggaa aaaataacaa cagcagaaaa
     9661 tgagggatcc cagccctgga atgcggattt tgcaggcatc ccatccggca tgggagagga
     9721 catggacggt cttctgacag cagcgggagt gaaggcacaa cattatgagg cgtctgagga
     9781 aaccacagaa gcacagccgt tgctgttgga ttatacctac gtacaaaata gtttacaggc
     9841 cggtgccgta gcagtgagag cccggaaaaa cagtgaaatc actgtgatcc aggattttgc
     9901 ttccgacagg gaggcagcag ggcaggcggc agtatccacc agactgtatc tggaagaagg
     9961 agcgaagctc cggctgatcc aggtgcagag actgggcagt gattttactt ttatgaatga
    10021 tatcggagcc ctgtgtgaag agaaggcttc cctggaagtg atccagctga tcctcggcgg
    10081 aaagaatact taccttggat gcaagactac cttacagggc agagagagca gcctgaatgc
    10141 cgacaccgct tatatcgtgg gcggtgacgg aagactggat atgaattatg tagctgtgca
    10201 tgaaggcaaa aagacccaga gcaatatgca ggccggcggc gttctgcggg atcatgcttt
    10261 caagctgtat cgtggcacca ttgatttcaa atggggggct aaaggtgcag taggcaatga
    10321 aaaagaggat gtactgttat tatccaacga tgtggtgaac cagaccattc ccctgatcct
    10381 ctgtgcggag gaagatgtgg aaggtaatca cggcgccacc attggcaaac tggacgatga
    10441 gctgctgttc tacctggaga gccgcagcat gaacagagag cagatctatg agatgatggc
    10501 tatggccaag gtggatgcgg tctgccgcaa gattcctgat gcagctacac gggctaaggt
    10561 acaggagttc cttggcagag ccggggaaga agaggaagcg gaagagtaag ataacaggag
//
LOCUS       DAWEJW010000095        10592 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2169_length_10592_cov_61.568948, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000095 DAWEJW010000000
VERSION     DAWEJW010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2169_length_10592_cov_61.568948"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2423 a         3060 c         2210 g         2899 t
ORIGIN      
        1 tttatgaacc cttatataag tgcgcccaaa aatctacttt atttttcact ctttctccgt
       61 gaaacatgct ctgcattgca gtcgcttata acagaaaacg gaccgcatcc gcagtccgca
      121 tttttctcaa aaggtgtaag caaaaaataa gccgagttct gtatatgacg atcatctatc
      181 tagtacgact gtcaccagcc gcatcaagcg acctacctga aagcagaccg ggccggtcta
      241 tagctctctg tttggtcttg cttcggatgg ggtttacatg gctccgtctg ttaccagccg
      301 gacggtagtc tcttacactg cctttccacc cttaccggcc aaaagccggc ggtatatctc
      361 tgttgcacta gccttggagt cacctccacc agacgttatc tggcatcctg ccctgtgaag
      421 cccggacttt cctcacctgc ataaagcagc cgcgatcatc tatcttactt acacctgttc
      481 gattatatct ttttcttccg gaaaaagcaa tatttttctg ccagacgtat gatttcccca
      541 tatcccagcg ctgccagcgg gaacatcatc atgtaatagg catacatata cctggccttt
      601 gcttcccaga aaatactgaa caaaaagccc ccgatcacag ccactgccaa cagatgctgt
      661 gtgatctcac tgtcttttcg gacacagaag ataaagtaaa acagacaccc cagatagata
      721 atgaactgta aacgatctgc catcctcacc acactgtcaa atagatctcc actcagccgg
      781 tcttccagtg ccgccagttt ctcatggtac acatccccgt ggttaaagtt gaaatacagt
      841 gactgatagg tcgggacatt ccactgggac agaagttttc cctgataaaa acctctcaga
      901 taccccggct gtgctttcat ggcctgcatg cgctcctgca tcatctcccg gtagatctga
      961 tcgctctgct ctgtattaca gtccgttgca taatattgtg cccagcaatc gctgtaatac
     1021 cagccgtacc ggccaccgat ctcttccatt cccagataca ggtaagccga tgtgggcaat
     1081 ccccgggaat gctccattcc ggatctcatc tcatacattt tatagattcc ctgatagcac
     1141 aagagtggca gtgccaacgc cagcaccagt gaaatcaaca ggcgtctgtc ccattttgag
     1201 aatatccgta ccgcgccgac catgcaatat gccaccacca ggaccagtgt gttcttacgc
     1261 agcagagttc ccagtgtaag agctgtcaca atgcccacca gtgatcccgt ttttccatat
     1321 ttgatatatc ggaccagcat ccatgcggaa aacagggaac agaataccca gggaatctct
     1381 ccgtagaccc agctggcata aaagacagat gccatacagc tgcccgcaag cagtgtcccc
     1441 atggccacca ccgcaagtct gccggaaact tccttcagaa tgccgtaaat gcaatagata
     1501 cccgccacgt tcagaagtac gaacactatc tggatcacat gataatccgc ctctccaaga
     1561 atccggaaca gcatctcttc catggccgcc ggtcccagct gatgagcata cagaccgcaa
     1621 taatgatccg gagccagcgt cccatagtca ccctgtataa aatctatggc cgcaccaata
     1681 accgatgcct gatctgcact cggcactctg tctaccgcca gaacccagca caatcctgcg
     1741 attccctgcc aaagcatgcc gagcgttacg gcaattcttt tacaccagat ggcgattttg
     1801 tcaccggcct ttttttccac tgccgtcaga actgcataca atagcaatat cagcgctgct
     1861 gccgccagat tccgtattac ggaatccgga atctccacca gaacttcatg acctgccacc
     1921 ggaagcatat actgcagggt acgcatggca taccatacaa ggaacagaag gatcaggctg
     1981 cccatgatcc ggatcaccca gtttgctgtc tgctttacta tcgattttat ctttggtaaa
     2041 cgactctcca caggtattct ccttttctta ctgtagagta aactattcag aaccatgcat
     2101 gtaaactatt cagcacctca ttcgcaaaat cgcctctccg aggcttcctt tttgctcatg
     2161 tggttacttc gtcgcatcaa ttctgccgca cagtcttacg aatgcaagca ttcgacgcct
     2221 gtcaggtaga tttgcgccgg tagccgtgta aactattcag caccacattc gcaaaatcgc
     2281 atctgacgat gcttactttt tgctcatgtg gttacttcgt cgcatcaatt ctgccgcaca
     2341 gtcttacgaa tgcaagcatt cgacgcctgt cagcagaatt gcgctactct gaatagttta
     2401 cacgttgaag cagcttccac ccacaaattc cctgcacaga gaattattgg gaaggctttc
     2461 actggtcaca cccaggaaat actccaggaa cttaagcagt ctcttggctt cgtaatattc
     2521 cggttctccc ttatcgatct ggaacaataa cggctctgcg aactgcttta acactgccag
     2581 ttcatagatc aggacggaat taaacatggc atccgcatcc tcctgaaacg ggaaaatatt
     2641 ctgttcctca ccccgtcgta cagaagccca catatcgatg gtgcgtctcg catccgcacc
     2701 tctggtcctc gcatctctga ccatacggcg gagaagtctt ccgtccgtag tcgggatccg
     2761 gttatgctca tcaatattga tattggtcaa tgcactgata tagatcttgt atttactctc
     2821 ctcgggaagt gcataagatg tcttctcatt cagaccgtgg ataccttcga tcaccagaat
     2881 atcatccggt cccagttgtt tgaaattgcc cttatattcc cgttttccgg tcttgaagtt
     2941 aaaggaaggc agctccacac gttcaccggc taacaatctg cacatgtcgt cattgaactg
     3001 ctttacatca atcgcttcca gacattcgaa attgtaatca ccgttctcat cccttggcgt
     3061 gaattctctg ttcacgaaat agtcatccag tgcaatggga tgcggtgttt tccccagggt
     3121 cttcaactga atggataatc tgtgggaaaa actggtcttg ccggaagaag agggtccggc
     3181 gatcatgaca aatttcacgc cgcccctgtc tacgatgctc ttggcaatct cgccgatctt
     3241 acgctcctgc tgcgcttcct gtaccaggat cagttcactc agcgacccac ggcagatctg
     3301 atcattcaga tcacctacgg tctcaatacc gatctccttt ccccattcct cagattcttt
     3361 caacgtccgg aataacagct ttctctcttg aaacggctta acatgtgtag gatctttttt
     3421 atccggcagc agcagcataa atccatcttc atacgctatg acatcgaacc actttacata
     3481 gccggcattg ggcagcatgt agccgtaata ataatcgtaa taaccgtcca tttcataaat
     3541 atttacgaaa gagcctctgc ggtaacggaa cagcttttcc ttatccttca tcccctcttt
     3601 ccgaaacagt tccatggcat tatccaggga ataggatctc ttcagaaaag gtgtctttgc
     3661 ctccaccagc tcccgcatcc ggttacggat ctttgcagca ttttcttccg tcgcattgat
     3721 cttccctttt ggagagatgt aggagccgtt gccgatggca aattccacac gacagctctg
     3781 ggccgcctct ctgccgtaga catcaaatac cgctttcaaa aacagcattg tggcagaacg
     3841 cacataagtc ttgttgccca catcatcctt caaggtaaaa aaggtaacct cgcaatccct
     3901 tgtcagtttc ttgaacaatt ctctgatctt gccgtctctg gctgccagtg cgatcagatt
     3961 ctcatactcc tgctgataat cgttggtcac atcctcgtaa gtggctccct gaggatattg
     4021 tctctcttcc ccgtttatct tcactgtgat catttttctc accctgtcct tctgtaatct
     4081 gcttttacag gtcttattgc ctaaatccaa tccgcttcct tgccattatc ataccaatcc
     4141 agtgcacggg taatcagggt gatctcgtct gcgatctcct gtctgcggca tatggttctg
     4201 ggattgtccg catctgcctt ttgctccatg cgtcccacga tctgccttcg ctcagtcagc
     4261 atccattcca gatactcccg aagtatccgg tctctgcggg ataaaagcag cgggccgaac
     4321 ttttccgcca gtccgggatc cagaccttcc gtcttccagg agtccccgac cttctgccgt
     4381 accgcatcgt ccgtcatcgc acccagccag cgcaccttca tcataaaata atattttcct
     4441 tcttcgtaga tgatattctc atccagcaga agatatccct catccatcag gaatgtccga
     4501 aaatgcggga tctccgactg tggctgcagg atcagctctg caaatccctg tgcctgttct
     4561 cttccttcgg taagaatctt ctccatgaga ggtccgccca tgccggcaca gatcatcccg
     4621 ttcgcctctc cggtcttcag gccgtgcaga ccgtcggaaa gtctggtctc tatcctgtcc
     4681 tgcagaccat attcccggat atgctctctg gcacgctcca ggggccctga ccgcacatcc
     4741 atggcgatca cccgtgggga gagccccttc tgcacgagat agaccgatac aaaaccatgg
     4801 tcacacccca catctgttat cacagtgccg ggagtgacca tgtctgttaa agcctgcatt
     4861 cgattggata atataacttc cgccattaat ccagataatc cttcagcttg cggctgcggc
     4921 tgggatgacg cagtttgcgc aatgcctttg cttcgatctg acggatacgt tcacgggtaa
     4981 cgttgaattc cttacctact tcctccagag tacgggcccg tccgtcatcc agaccgaaac
     5041 ggagtctcag tactttctgt tctctctctg tcagagtacc cagcacttcc accagctgtt
     5101 ccttcagcag ggtaaatgct gcagcatcgg aaggaaccgg tacattatca tcctgaatga
     5161 agtcgcctaa atggctgtct tcctcttcac cgataggagt ctccagggat acgggctcct
     5221 ggctgatctt taagatctca cgcactctgt ctaccggcat gttcatctct tccgcgatct
     5281 cttcgggggt cggttctctt cccagctcct gcagcagctg tctgctcacg cggatcagtt
     5341 tgttgatggt ctctaccata tgcaccggga tacggatggt tctcgcctga tcggcgatcg
     5401 ctctggtgat ggcctgacgg atccaccagg tcgcataggt ggagaactta tatcctttac
     5461 gataatcgaa tttctctaca gctttgatca gacccaggtt accttcctgg atcaggtcta
     5521 agaacagcat tccacggcct acatatctct tggcaatact gactaccaga cgtaagttgg
     5581 cctctgccag acgctttttc gcttcctggt cgccctcttc cattctctgg gccagttcga
     5641 tctcttcctc agcacttaac aggggcacct tgccgatctc ctttaagtac atacggacag
     5701 gatcctcaat gctgacgcct tctggtacgg acaggtcgat gttctccaca tccacttcgt
     5761 cctcatcggt cagaatgatc tcttcgtcat ccacatcatc gtcatcggta atacgcagca
     5821 catctacgtt gttctggtcc agatactcca gaaccttctc catctgttcc tcttccagag
     5881 gcatgtccgc aaagaagtca ctgatttcct gatactccag cacattcttt ttctttttcg
     5941 ccatattcag gagctctttt atcttctccg caaattttgc ctgtgtgttt tcatccattt
     6001 tcgtggttcc atcctttcat tgccattatt tatagccata tttcgtattt tttataactg
     6061 tcccgagccg caaaccctat tattccagag aaatgcgggc tctttgtaat tcttccagtg
     6121 cttttttgcc ttccaccgct tttttgaccg cgttgacatc tgtacccagc tgcgccatat
     6181 agtactggta gctgttgcgt ttgactgaca ccagaatgtc atggaaagct ctttctctct
     6241 cctgcttcgt ggacaattcg gggagcgttg tatggaacag ctccgctgcc tgcctctgct
     6301 cctcttcatc ctcaaacatg ctgatgatcc ccgccggctg aaactgtccg ttgtccagct
     6361 ctgcaaacat cttctccgcc accttacgat acaattcctc cgtgaaatcc tccagagaaa
     6421 tgtacttacg tacaatcgta tacagagaag gatcatctcc gatccaggtg atcagcagtc
     6481 tctgggactt ccgtccgctc tccgaaggat cgctcttgtt ctgcactccg ctgcggggac
     6541 ggggaatcgg tctggcaatt cccgtctggg aagcatatcc tatgaccatt ttgcgcagat
     6601 catcgggacg gatcatatat ttttcggcaa tggcctgcaa ataattctcc cgctccagat
     6661 cttccgaaaa ttcgcacagt ttctgtgcga tctcccggtg gaacttcgtc cgctgttccg
     6721 gatcactctg atcgtattgt tcactcagga tacggatctc gaagtagaaa ctgttctccg
     6781 cctgatcgat tctctcctga aaagcctctt tccccagatt cttgataaat tcatccgggt
     6841 ccttataagg acgcatattg atgactttac cggtaagccc ggcctccctg agaataccga
     6901 tgccccggag tgccgccttg gttccggcac cgtcactgtc gtaggccaac aggatgtttt
     6961 ccgcataacg gtgtaaaagg ttagcctgtt ccaccgtaaa tgccgttccc agggatgcca
     7021 ccgcctgggt aaatcccgcc tgatgcatgg ctatgacatc catgtagccc tcgcacagga
     7081 taatattgcc cgtccgggca gtccttgcga aattcagccc ataaagattc cttctcttat
     7141 cgaagatcat ggtctccggt gaattcaggt atttcggttc cccgtctccc atgactctgc
     7201 cgccgaagcc gatgacccgg tggttgatat cctgtatggg gaacatgaca cgattccaga
     7261 actggcttcg cagtccccgc tgttcagaaa acattgccag tccggagtct ttgatctctt
     7321 cgtccgtaaa gccctttttc cgcaggtact gtaccacctg ctcgccgttt ttcccggcat
     7381 aacccagtcc gaaatgatgc atggtctcat cggtcagctt ccgccccgtc agataccgca
     7441 ttcccacttc gccacgggga tcccgcagca tataataaaa atattttgcc gcttccttat
     7501 tgacttccag cagccgcata cgtttgccgg cttttttctt ctgttccggt gtctcctcca
     7561 cttcgggaag ctttaccccg gcgcggtccg ccaggatctt aatggcctcc gggaaggtat
     7621 cgttttcata tttcataaga aacgtatata catttccgct ggcatggcat ccgaagcaat
     7681 aatacatctg tttgttcgaa gacaccgaaa aggaaggtgt tttttcgtta tgaaaaggac
     7741 agcagcccca atagttgctt cccttttttt tcaatgagac atagcccgat atcacatcca
     7801 cgatgtcatt tctggctctg acttcttcta ccagttcttc gggataatac acgtttttta
     7861 actcctattt tatcttgtac ctatctctgc caggatttcg gtatgtaaat atcctcgtat
     7921 accgctatgg caaaccgatc cgtcatcgct cccacatagt cgcagacgac cttctctctc
     7981 ggttctccca cggaaagaag attgagatat tcttccggca gatcatccag atgcttcagg
     8041 aaatgctggt acaatgtctc catcagcatc tctgctttcc cctcttcgct cttcgctatc
     8101 gggttcttgt aaactctctc aaacatgaac tgtcgtatct tcgtcatggc ttccgccacc
     8161 ggctccgaca tcaggatgtc tcccttgtcc atgctgttgg tcacgatgtc atgaatgaaa
     8221 tgatccagtc tctcggtggg actgctgccg atcacctcac agatgtcctt cggcacatcc
     8281 gcttccgtga ggattcctcc gcggatggcg tcatccatat cgtgatgaat ataggcaatt
     8341 ttatcggaaa gtctgacgat cttgccctcc agggtgtggg gctttccaat ggtctggtgg
     8401 tttcttatac cgtctcgcac ctcgtaggtc agattcagcc ccttgccatc cttctccaga
     8461 atgtccacgg tgcggacgct ctgttcggca tgttcgaagc caaaagggca tactctgttc
     8521 agcgcacgct ctccggcatg tccgaaaggc gtgtgtccca gatcgtgtcc cagcgcaatg
     8581 gcttccacca gatcctcgtt cagctttaat gccttggcta tggttctggc cgtctgggat
     8641 acctccagcg tatgtgtcag tctggtacgg taatggtcac cctcaggtgt cagaaatacc
     8701 tgggtcttat cctttagcct tcgaaaggcc ttgcaatgga taatacggtc tctgtctctc
     8761 tgaaacaccg gccggatatc acattgttct tccggtcgca gacgtccttt ggagttcaca
     8821 ctcaaggatg cgtacggact taaataatcc ttttcccact gttccaagtt ttctctgatc
     8881 gtcatagttc ctcctgcccc tacttctaat attctgtgcc tgtcttaaaa atccttttct
     8941 ttcttttgaa aaaaatagtc attttttctg atttaaatta tctgcacaca gaatagatga
     9001 attccgcatt cttatccatg gcctcactgg cctttttgaa gggtgacatc tgaaaaacat
     9061 gccacattcc ggggaatcgg tcgatatggg cagcgacccc ggctttgatc agatttttat
     9121 atagccgcag agaatcgtcc aacaggattt cattctctcc cacctgaata tagacgggcg
     9181 ggaaaccatg aaagtttcca aacagcggag aaatataagg atccgtcaga tctctgtccg
     9241 gggcataggc cctggtcata cggtcgatgt actctttatc cagtaccgga tccagttccg
     9301 ccttggtctg gaaagtcttg ccctctccgg taagatccgt ccagggagac atcaacacga
     9361 ttcctctggg aagcaagcgc tcctgctctt tcaatttcaa caccagtgaa agcgccagat
     9421 tacctccggc cgaatctccg gtaatgatca catctcttgc tccgtagccc aaaagcatca
     9481 gatagttcca ggctttcatg gcatcctccg cagctgccgg atacggatgc tccggtgcca
     9541 gtctgtaatc aaagcacagc acatccatgg aagtacccgc cgccagcttc gatgtcaggg
     9601 ttctggcata gatactgctg ccggtggaat atccgcctcc gtggcaatgc aggatcacgt
     9661 actttttcat atgagcccgg ttcacgctga cccactccgc atccatgcca tcgatgtcca
     9721 cgggacggta ctggatctct ttactgttgc cgagcagctc gccaatgtga tcctgggatt
     9781 gtctgtgctt ctccagatcc ggattctcca caagaccatg ggccctgcgg atgagctgca
     9841 tcagattctg gttgttcttc tcactgtttt ttcctaccag tgccataagt tcctcctgtt
     9901 cacctagttt tctcgtaact attcagcgcc tcattcgcaa aatcgcatct ccgatgcttt
     9961 cctgaacaac aagttcactt gttgtttgtt catctgaggc tgcttcgcca aagaaatatt
    10021 gtccagtcgt cttacgaatg cgagcattcg acgaggcttt gccaatattt catggctctg
    10081 aatagtgatc aaaagtgcaa tcttctgcgc actgcattgc gcagtctcct ccgggataaa
    10141 agggtggcga tggtgatatc gacgatcacg cagatcgtca gcacgatagc cgaccatccc
    10201 agtgcgatct cctgtctcag gaaccggtcg atcagaagtt ccagtcccac gcacaaaaca
    10261 gcgattcccg agatcaggta cagggctcct gtcaggaagg acatggggag cttgcggata
    10321 cagaacatca ccgcttccac cactgccgtc accagcaata cgatcggcag tcccagtcca
    10381 tagaaccagc tgttatgtcc cgtcagataa gtcagcagat atagatacac gatcactgcc
    10441 actccatcca gcaggatcga cagataaatg ggctgtctcg tataaataac cacagggatc
    10501 ataataaccc acaataccag gactacgccg atcaccgcca gagaccacag actgctgcgg
    10561 aacacaaaag cgtttaacag cccacaggtc ac
//
LOCUS       DAWEJW010000096        10584 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2173_length_10584_cov_45.831418, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000096 DAWEJW010000000
VERSION     DAWEJW010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10584)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10584
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2173_length_10584_cov_45.831418"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2968 a         2140 c         3007 g         2469 t
ORIGIN      
        1 ggcagaactt gtggacctat tgaaaaccta tgccaatcat ccctcttttg taatgctgac
       61 actgggcaat gaattgcagg cacaagatga aggcagagaa cggatgcggg aactggtcag
      121 aacggcgaaa aggatggatc cgaccagact gtatgcgaac ggatccaatg ccttttatgg
      181 tgaagaagga tgtgacccag agagcgattt ctatacatcg cagagttgca aggatgtggt
      241 gatccgcgga acattctccg gcatgcgggg gtatctcaat gaaaactatc cctccgcaga
      301 tcgaacctat gatgaggcga tggcagagat acgaaaagag tatcagaagc cggtattcag
      361 ttttgaagtg gggcagttcg aagtgctgcc ggattttgag gagctggaaa gctttcatgg
      421 aatatcggat ccggtgaatc tgaaactgat caaaaaacgt gtagaggaac ggggactttt
      481 gcccacatgg gaaaaatacg tagaagccac cggagaactg tcgaggctgg cataccggga
      541 agagattgaa gcagctatgc ggacgagaga gctttcaggg atttcgctcc tcggactgca
      601 ggatttcccg gggcagggga cggcactggt aggcatgatg aattcccatt tagaaccgaa
      661 gccctatgat tttgccagac cggaaaggtt ccgggaattt tttcaagagt gtaggatttt
      721 ggtaaaactg ccacattata cctatgaggc aggagaacgg ctgatcgcag aagtggaagc
      781 agcgaatttc ggcaagagaa atatcgaagg agtattttgc tggacccttg cgggaaaaaa
      841 gagtgtgtct gagaatggaa actgtgagcc ggcggagata aaaagcaaaa atacagttat
      901 cgcaaccgga gaagatacag agataacaat ctgccgccct ggaagttata cagaggtagg
      961 aagtctggat attccgctcg actttgtgga gaagaatacc gcactcacgc ttaaggtgcg
     1021 catcggtgac agtatcagtg cttatccgat atgggtgtat cgaaagacca ctccggtatg
     1081 cccggaaaat gtatatgaga ccagagcctt tgatgtgaag acgagagaaa tccttcaaaa
     1141 tggtggaaga gtctatctgt ctccggatgc agacaaagag agtctgccca attccattaa
     1201 aacacagttt acgacggatt tctggtcggt gggaactttt gcagatcagg aaggcggtat
     1261 ggggcagctg atcgatacgg agcatccaat ttttaaggaa tttccgacgg attttcacac
     1321 ggactggcaa tggtggatta tggcaacgaa gcgggcggtg attctaccgc atcccatgaa
     1381 gacgatcatt acggagatgg acagctatgc attcttacgt cccatggcac agatgataga
     1441 gtttcgatgc ctgaagggaa aagtattgct atccaccatg gaattgcata agagtcagca
     1501 gtatccggag gtgagggcac tgcaggcgtc aatttatacg tatctgtcgg gagagaattt
     1561 tgagccggcg gaggaaataa cggaagaaga gttaagcatg ctggtgcgag gttaaaataa
     1621 tatcgggcgg cggaaagaaa gctgcacgga gataaaagga ggaaaccatg aataaggttc
     1681 aggtctggga agaaaaagtg atcattccca catacgaagc aggaaagccg gataagaatc
     1741 cgatgttttt ggagaaacgt gtctatcagg gaagctcggg aaggatctat ccccacacgg
     1801 tgattgagaa gatcagcgat gagaaagtgg ataaggagta tacagcgctt tttttggaga
     1861 acgattatct gaaagtaatg atgctgccgg agctgggagg caggatccag agagcttatg
     1921 acaagacaaa tggctacgat tttatatatt acaatcatgt catcaaaccg gcgttagtgg
     1981 gactggcggg accatggatc tccggcggta ttgagttcaa ctggccgcag catcacagac
     2041 ccagtacctt tgatcaggtg gaatatacct atgcggaaaa tgaggacggg agcgccacag
     2101 tctggatggg agagattgag aacatgttcc ggaccgaagg agtgctgggc gtgaccctgt
     2161 atcccgacaa ggcatacatc gaattgtctg taaagctata taaccggacg aaaatgcccc
     2221 agaccttcct gtggtgggcg aaccctgcag tagcggtaaa tgatgacacc atctccgtat
     2281 ttccggagga tgtgacggcg gtctatgacc acggcaagcg ggatgttatc agcttcccct
     2341 atgcggaggg aacttattat aagcataaat acgaccatgt gaatattgcc cagtataaaa
     2401 atattccggt gcccacctcc tatatggcat atcgttccga ttacaatttt ataggggagt
     2461 acgattacgg aaaacaggca ggacttctgc atgtggcaga tcatcatatt gctcccggga
     2521 aaaagcagtg gacctgggga tgcggagaat tcggcaaagc atgggacctt gcactgaccg
     2581 acgaggacgg tccgtatatc gaactgatga caggctgttt tacggataat cagccggatt
     2641 tcacatggat ccagccccag gagaccaaga atttcaaaca gtattttatg ccctataaaa
     2701 atatcggcta tgtgaaaaat gctacgatcg atgcggcggt aaatgcggaa tatgatgaga
     2761 caaaggggca gcttacggta tcggcctata ccacttccgt gcaaaaaggg gcacatatac
     2821 tgctgacatt gccgggagaa aacggcagac aggaaaaggt actgtatgag gaaacctcag
     2881 atctgtcacc ggaggaaact tatgaagtaa agatcgaccg tgaaaaattg caacagatac
     2941 cgacatttgc agagggagag cagaatggaa cagaggtact ctgcggtctg cgggtatgtg
     3001 tttgtgccgc agatgggaga gaactggtct cttaccattt tccgaaaaaa attgaagcgg
     3061 aagtgccgga accggcgaag gcagcgctac ttcccgaaga ctgtgaaacc acggaggatc
     3121 tgttcctcta cggtctgcat gtggaacaat accggcatgc cacttatcat cccgaggatt
     3181 attatctgga ggggctgcgc agggatccta ccgatatccg tctgaacaat gtctatggaa
     3241 gatgtctgct taaaaagtgt gattttgccg gggcagaaaa gtattttcgg aaggcagtgg
     3301 agaaatccat tcgttccaat ccaaaccctt acgattatga gccgtactat aatctgggac
     3361 tttccctgaa atatcaaggg aaagaaaaag aagcctatga tgtcttttac aaggccatct
     3421 ggggtggcag cttccaggca cccggattct atgaactggc ctgtctggat gcaaaagcgg
     3481 gaagattcgc agaggctctg gagcatgtaa atgaatctat tctcagacag taccagtgta
     3541 tgaaggcccg tgcattgaag gaaaatattt taagaaaact gggaagagtg aaggaagcag
     3601 aagaattaca taaggagagt ctgaagatcg atccgctcta tgacagacag ccggagaaaa
     3661 tcaaccataa caccctgctg gagctggtga tcgatctcta tgaggcagga gactacgcac
     3721 agggaatcac ccttgccgaa aaatgggtga aacaacagaa tgtatcacgg caagaggata
     3781 tttatccgct ggtatattat tatattgcct acggagacca gaacctgcat aggaaagaaa
     3841 atacggaaca ggcaaattgt tacatgacaa tgtacgtgac attgggagaa aatgctgcag
     3901 cggatggctg ttttcctcac agaacggaag attatatcgt gttaaagagt ctggcagaag
     3961 aatatcatct ggccatggct tactattatc tgggatgcct gctctatgac aggagagtgt
     4021 acgacgaatc catagaagat tatgaggaat cgattctccg gagtccggat ttcccgacgg
     4081 tacacaggaa tctggcactg gcatattaca atgtgaaaaa gctgccgcag agagcgtacg
     4141 aggaaatgga gaaagctttt gcactggatg agacggatgc cagagtgtat ctggaactgg
     4201 atcagctgaa aaagcgtctg aatgttgccg tgaaagaacg atggagtgat atggaaaaac
     4261 attttgacct ggtggaaagc agggatgatc tgtatctgga gtatgtgaca cttttaaaca
     4321 ctctgggaga acctgagaag gctctcggcc tgatcaaagc aaggaagttc catcagtggg
     4381 aaggcggtga aggcaaagtg gcagcccagt acctgaccgc cttgtatcag ctggcaaaag
     4441 cggctgtgga aaaagaggac tatgcggaag caaaggaact tcttctgcgg gcagtcgggg
     4501 aatatcctca taacctggga gaaggaaaac tagagtcagg gcaggaaaat aatctgtact
     4561 atctgctggg actggtgcag gaaaaactgg gtgaagcaaa ggatgctttc gagagcctga
     4621 caagggcctg ttgcggagag tcagaaccgg tgggcatgat gtattataac gatcagccgc
     4681 ccgaaatgat ctactatcag ggacttgcat accgaatgct tggggaagaa gaacaggcag
     4741 ttcgcagatt ccaaaagctg gtggattatg gcagagaaca tatcagagat gatgtaaaaa
     4801 tagattattt tgcggtatct ctgccggatc tgctgatttt tgaagaaaac ctgaacgagc
     4861 ggaacagaaa gcattgcctg ttcatgatga gcctgggact taaaggactg ggacggatag
     4921 acgaagcgga aaagtgtgcg gaagaactac ttgccatgga caatgctcac caggaaatcc
     4981 gggtacatga tctgtaaaaa agcttgaaaa gaaagacttg aataggaagg tttttgagga
     5041 aaggattaca aaatggaaaa catgggaaaa gaggtatata gctataacag cgaatgctta
     5101 acctgcaacg acagaccgat cctgcccatc atgggagaat tccatttctc ccgctatccg
     5161 gaaggggaat ggaaaacagc acttaagaag atgcaacagg gcggcgtaga cattgttgct
     5221 gcgtacgtgt tttggatcca ccatgaggaa gcggaaggtg agtgggattt ctccggcaga
     5281 aggaatctga aggcgttttt gaactgctat caggaagtcg ggatgaaagt atggctgcgt
     5341 atcggtccgt gggcgcacgg agagtgcagg aacggtggct tcccggactg gctggtgaag
     5401 aaagaaaagc ggggagaact ggctcttagg acgaatgatc cagagtatct gaagtatgtg
     5461 gacatctttt ttaccaagat tgccgagcag gcggatgggt atatgcataa ggatggcggt
     5521 cccgtcatcg gtattcagat cgagaatgag tacgggcatg ccggaggtcc ttccgacagg
     5581 gaagagggca tggcgcatat gcacacactc agagctatgg cggaggaaaa gggactgacc
     5641 gctccctatt attcggcgac cgggtgggga ggcgcttatg taccggaaag cttcctgccg
     5701 gtgctgggcg ggtatgtgga tgctccgtgg gcgaatcata tccacgaact tgcggcaagc
     5761 gagaatttcc tgtttcagcc ttttcacgac gatgccaata tcgcatcgga tttcgccgaa
     5821 ggacagagtg gttttacctt tgatacgtca aaattccctt acctgacggc ggagctgggt
     5881 gggggactgc aggtgacggc acacaggcgc acttacccgt atccggagga catcgaggca
     5941 cagacgatct gtatgctggg agccggggca aatctgatcg gatattatat gtatcacggc
     6001 ggcgtgaatc ccgacgggaa atattccacc ctgcaggagt cgaaagctac cggctatgcc
     6061 aatgaccttc cggtgaaaag ctatgatttc cagacctgcc tgcgggaaaa cggactgccg
     6121 tcggaaagct attacagact gcggaaacac catgttttta tcaaaaatac agaagaactg
     6181 ctggcaccgg caaaagcata tctgccggaa aatataccgg ggcctgcggg tgcagaggat
     6241 atggagacac tgcgggcggc cttccgttat aatgaagcag cggattgcgg tttcctgttt
     6301 attaataatc accagagaaa acggaaaatg acggagaaac agatcacacc ggaaaagcca
     6361 ttacagttta cggtgacaga tgttgaagga atacagaggc agattatttt cgaccggatt
     6421 catgtgcgga cggatgcgat tctggtgctg ccttacaatc tgccggtggt aatacgggga
     6481 gagcagttca gacttcggaa aaccaatgcc tcttatctgg gatgctttgg cggaacttat
     6541 tatttctata cggatgagaa gccggaggat atttatttcg agtggagtga tggcaatgat
     6601 catgcggaag cggtaaggat ccttaccata cacgatgcgg agtatttttg ttatgcgcag
     6661 gaaggggcag atgaaaaggg caaggtgagt ctgctgccgg atctgcactt tgcggaagcc
     6721 gataaggtgc ggatcgcgga tgccggacag gcagtggaaa gcatctggaa cgtatatggt
     6781 caaacagaac cgaacgtcta tgaattaacc ttggaatatg agtatcaccc ggcggatgca
     6841 ctgtctggcg atgtctggct ggaactggat ttcggtgggg actgcgcacg cttgtatcag
     6901 gacggaaaac tgattgacga ctggttctcc aatggtgaac tatggcgtgt ggctctgaaa
     6961 cgctacggct gcccgacaca actgaccctg gaactggatc ctttcaaagc ggatgtctat
     7021 tatgatctgc cgccgaagcg ggagaaccgt ctggcagggg cgagactgct gcggttaagt
     7081 tgaccggaac ccggtttggc aggactcact ttggaaacag aaggatagaa aagaggatac
     7141 agatatgaaa gacatgacac agggaaaacc gttaaaactg atcatcggct tcgccatacc
     7201 gatgctgatc ggcaatatct ttcagcaggt atataatgtg gcggatacag tgatcgtagg
     7261 aagattcttg ggagagagcg cgctggcggg cgtgggaagc acgggaacgt tgacctattt
     7321 tctgctggca ctggtcagcg gcatgtgtaa cggcgcagga ctggtggttg cgcagtgttt
     7381 cggctgcggg aataaagagc gtctggcgaa gacggtgaca gcactgatct gggtggcggg
     7441 agtgctgacc tgtatcatgt cactgatcgg tattttcggt gcggaatttt tcctgcggct
     7501 tttaagcgtg ccggaaaatg ttatcggcta ttctgctacg tatctgcata tcatttatac
     7561 ttttgttttc ggcagtgtgg tctacaacgg ctgtgcggcg atcttaaaca gtgtggggga
     7621 ttcgaagact ccgctgcggg cggtgatcgc ttcctcggta gtgaacatcg tgtttgacct
     7681 gtttttcatc attgtgtgta agatgggagt agcgggagca gcttatgcga cgattctggc
     7741 acagtgtact tcggcggtga tctgttttgc aaaagtcatg aaggggagag aagagattgg
     7801 cctgtctctg atgaaatgga agccggaggc tggttatatc cggttggtag tgaagaccgg
     7861 attcccggcg gcattccagt cctgcatgat cgcactgggc ggcatgagtg tccagagact
     7921 ggtgaattcc ttcggctcgt ccaccatggc ggcctacgtg gcggcaaatc gtgtggacag
     7981 tgtggcgatc caggtcatcg tctctattgg aacggcactt tcggtattta ctgggcagaa
     8041 cattgcgaag aatcagtatg accggatccg ggaagctctc tataagacat tgggtgtcat
     8101 ggtgggagcc agtattttta ttgcctccat ggtacttatt ttccgggttc agttaatgac
     8161 gctgtttttg gatgcgaacg aatcgggaga agccatcggc atcggctgca cctatctgac
     8221 cattatcggc gtggcttatg tgatcgcggg ggtgatgcag agctaccaga atgtgatccg
     8281 gggagccgga gacgtcaata cctgtatggt agcgggactg acggagctgt ccggcaggat
     8341 cgtgttcgcg tatctattgt ccggaaccct gggcgtcacg ggaatctgga tcgccactcc
     8401 tctgtcctgg agttgtggct gtgtgattcc ggtgatccgg tattattccg gcaaatggaa
     8461 gcacaagagg ctggcctgat gaccggagtg accggaacac agaggggcta ttccggccgc
     8521 atccatccgg caaattccag aatggactgg ttctcagcct ttttctcgct ctgctgcatc
     8581 tgcttgcggt attctctggg agacatctgc ataagtttga taaagtagcg gttgaaactg
     8641 gacacggaat ggaaccccac catctcggaa atgtccagaa tagagtgttc cgtactgcgt
     8701 aacagaatac aggattttaa aatccgggtg ttgttgacgt agtctaacgg tgaagttccc
     8761 ataatatcat ggaaaacccg gcggaagtgc gtcgggctcc agtggcagag atcggccaga
     8821 tattcgatcg agaactgttg catataattt ttctcaatat attccagagc gggtgagatc
     8881 accagagagt tttcggcgat ctcgccttct tttttacgtt cctgtaaggc cagtatttca
     8941 agagagtccg gtttggccgt gtcgggattg acaccgccga gactttcgat ccggtacaat
     9001 tcaatataaa gggagcataa caggcttctg gcactgatct gatagcaggg attctgctct
     9061 tccagctcgt ggatgatatg agataccaga tagttgatgt ggggaaaagc ctctttgctg
     9121 aaaatataac ggaatgctgg gaaaccgtcc gtggacaggt cgtaattttt ccaggaggcg
     9181 gggaggagat tgcggaacaa ttccttcgga tccaggaaaa gataagacca gtggcttttc
     9241 gtgcctggtg cactgtaagt ggtatgtgga acattgcggg gaagaacggt gacatctccg
     9301 gcatggaaag tctgtttttc tccgttgatc tccagcgtac cgctgtcgga atgacagatc
     9361 ccaatctcca gacagttatg aaaatgaagg cttccgctgg gaatatcgga aatcttccaa
     9421 taatcaccgg acagaagcag taccgggaag tgtgttggca gataataact tcggtattct
     9481 gtgacagctc gtttggattt cgccatagtg tgcctcctgt tccagagttt tctgaatagc
     9541 tttgtgcatc ctgtatgatg atgcaacgga aagacatgct gcagtagaaa taatgtctat
     9601 acattatata taacaattaa aatggataaa atcaaccatt atgttgcgaa aatgactttt
     9661 ttagggttgg caatgtttcc gtggaagaga ttaaaaaagg ctaaaaatac cgtgaaacag
     9721 cgaatcctac agcaaaaacg ctccaaaatt cacgcaaaat ttacgaaaat gcaacgaaac
     9781 ttcaatactt acgaaaaacg accatgatct ataatgatcg tttttgcata gttttttata
     9841 atgattgatt agaaaaatat gacaacatgt atgataatgt acttacaaag ttacaaaaag
     9901 tgtacaaagt gacgagaaaa tatagctgac acttgaggag gttcgacatg gcgaagaaga
     9961 atgcagccaa tgtggtggga gtgaaaacga aaaccacgtg gaaacaagct tttaaaagag
    10021 actggcaatt gtatctgctg ttactgcttc cactgattat ggtatttgta ttcagttacg
    10081 gagcataccc gggattgcgg atggccttta tggattataa accggcgaag ggatatgcag
    10141 gcagcgaatg ggtgggcatg aagacattcg ttaagatatt caaggatgcc gatttcatga
    10201 gagcacttcg caactccata gtgtttaacc tggcggatct gatcatcggt ttcccaatgc
    10261 cgatcattct ggcactgatc ctgaatgaat taagatatcc gagattcaag aaagtgtccc
    10321 agacgatcct gtatctgccg cacttccttt cctgggccat catcggaagc gtagccatga
    10381 caatgttccg tccgaactcc ggtctggtga acattctgct gaccaacctg ggagtgatct
    10441 ccgaaggaat tcctttcctg aatgagaaat ggcactgggc aattacatat ctgctgatcg
    10501 gtgtatggca gacgatggga tggggcacga tcctctacct ggcagcgatc accggtatca
    10561 acggagaatt gtacgaagca gcca
//
LOCUS       DAWEJW010000097        10382 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2231_length_10382_cov_57.543914, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000097 DAWEJW010000000
VERSION     DAWEJW010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2231_length_10382_cov_57.543914"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2350 a         2866 c         2116 g         3050 t
ORIGIN      
        1 gtgcttcgca cttatgattt tctcgataaa cacccataaa aaacaaatgc cacacccgaa
       61 ggtgtggcgc ttgttttttc tatgggtgtt tacatcattc cgcccatgcc tgcgccgccg
      121 gcgggcattg cgggggtgtc ttctttgata ttagctacaa cgctttcggt agtaagtagt
      181 gtgcttgcta cgctggtagc gttctgcaga gcgcttctgg taaccttagc gggatccagg
      241 atgcctgcgc ttaccatatc tacatattcc tctttcagag catcgaaacc gacaccgacc
      301 tcagactcac gtaccttgtt gatgataacg ctgccttcca gtcctgcgtt tgctgcgatg
      361 tgatacagag gtgcttccag tgctttcagg acaatgcctg caccggtctt ctcatcacct
      421 tccagacttg ctgccagttt ggcaacttcc ttggatgcat gaatgtatgc ggaaccgcca
      481 ccacagatga taccttcctc tacggctgct ctggtagctg ccagagcatc ttccagacga
      541 agcttagctt ccttcatctc ggtctcggta gcagcaccta cacggataac tgcaacacca
      601 ccggacagct tagccagtct ctcctgtaat ttctctctgt cgaaatcaga agtggtctct
      661 tcgatctgct tcttgatctg tgcgattctg gactgtaatg cttccttatc gccttcaccg
      721 tctacgatca cggtgttctc tttctgtacc ttaacggatt ttgcacgacc cagcatatcc
      781 attgtggtat ctttcagttc ataaccaagc tcggaactga tcacggtacc accggtcagg
      841 attgcgatat cctccagcat agcttttctt ctgtcaccat agccgggagc cttaacggct
      901 actacgttga aagtaccacg cagcttgtta acgatcaggg tggtcagtgc ctcgccctct
      961 acatcctcag cgatgatcag cagcttagcg ccggactgaa cgatctgctc taacagaggc
     1021 aggatctcct gaatgttgga gatcttcttg tcagtgatca ggatataagg gttatccaga
     1081 acagcttcca tcttatccat atcggttgcc atgtaagcgg aaatataacc tctgtcgaac
     1141 tgcatacctt ctaccagatc cagttcggtc tgcatggtct tggactcttc aatggtgata
     1201 acaccgttgt tgcttacctt ctccatagca tctgcaacca gctgtcctac agcctcatcc
     1261 cctgcggaga ttgcagctac tcttgcgatg tgctccttgc cgtttacttt ctggctcatc
     1321 ttaccgatgg cttctactgc acatgcggtt gctttcttca taccttttct caggatgatg
     1381 gggttagcac ctgctgccag gttcttcatc cctgcattta ccatagcctg tgccagaacg
     1441 gttgcggtgg tagtaccatc accggctaca tcgttggtct tggtagctac ttcctttacc
     1501 agctgtgcac ccatgttctc gaatgcatct tccagctcga tctcttttgc aatggtaaca
     1561 ccatcgttgg tgatcagagg tgctccgaag gacttatcca gtactacgtt tcttcctttg
     1621 ggtcctaagg ttactcttac ggtatctgcc agctggttta caccagcctc taatgctgca
     1681 cgggcttctg cgccgtattt aatctctttt gccataataa tcagcctcca atttatttgt
     1741 tcttagtctt aatttctttt taggtttgca attactgaat gattgcaagg atgtcgctct
     1801 gctttacgat gatgtactct tcatcatcca gctttacttc ggttcctgag tatttggaat
     1861 agatgacctg atcgccaacc ttaacttcca tctttacttc cttaccgtct acgacaccgc
     1921 cggggccgac agcaacaact tctgcctgct ggggcttttc cttattctga ccggggagca
     1981 cgataccgga cttggtagtc tcctctgcaa ctaactgttt taatactact ctgtcaccta
     2041 aaggtttcaa actcatgaca actgcctcct tataactatt taaatatcat attactcttt
     2101 ttcatttgtt agcactcatt tttatcgagt gctaatttct atagtttata ttagttttat
     2161 atgccgagtt tttcaaacgc attcaccgtt atttaaataa ctatttcttt tattttctcg
     2221 ttttatctca gtttttctaa tgtttttata ttacatgtgt aaaaatgcga tttaatattt
     2281 cgtttataat tctgaaaatc catatccggt tcatcccttg caaactcctg tgcagacata
     2341 aagctatacc ggttacatcg tataagcaaa aagcagccgg ggatcttatc attatcccaa
     2401 gctgcttctg ttccgctctg tatttacttt cccgtcctat gcgttcttgg attcctcatg
     2461 ccatttctgc cacttcttct tataaataaa gtagcagggg attcctacga ataccattcc
     2521 gcctaagata ttgccgatgg taacgggaat aaagttgttc aggctgtgca gtactgtcag
     2581 tccctggatc tgctctgcgg tcaggccgta tgcttcctgt gcctttgcaa catacgcggg
     2641 attctgtgca gccatcatgc ctgcgggaat gtagaacata tttgcaacac agtgctcgaa
     2701 accgccgact acgaaggcca tgatcgggaa gaagatcgcc cagatcttac cgacgatatc
     2761 cgtagctgcc gctgccatca ggatcgcaat acataccaga atgttacaca ggataccgga
     2821 ggtaattcct ttcagcggag aaatgcctac cttgccgact gccactttga tggcataagc
     2881 ccccagcaat ccgccggtgt agtccagatt tccggaaaag cagatcaggg tatcaatgat
     2941 cagcgcgccg atcaggttgc tgaaatatac cacgatcaga ttgcggatac attcgaacca
     3001 ggtcaccttt ttgcctacca cgtccatgac gatcaggcag ttgccggtga aaagttctcc
     3061 acctacaaat acaatgatca tcagtcccac agggaagatg ataccggcta cgaatcgggc
     3121 cagacctacg ttgtcaatag catgcgccgc agtactgctg gtagctccgc ccagtgcaat
     3181 gaaagcacct gccataatgc ctaacaggat catcttgccc agaggaagat gtgccttgcc
     3241 ctctccggcc ttcatgttat tatcaatgac ttctgccggg gtattaaaat gctgttccat
     3301 attttccctc atttccgtac agcccttatc tgccgtactt tctcatactc atttactacc
     3361 tatattacac gaaatctgcg gcgggaatca agcttttccc cctatgtatc tgattatata
     3421 catttcctct caccgttttt gtcataacac aatctttttt tgtaaaattt atgcaaaaaa
     3481 atagtagaca aaagccgaaa tgtgtaatat acttaggatg ttcggcgaga gccgaaccgc
     3541 ttatataagt agatttccgg aagaattccc cttttacaca attaaagtgg ctgcaggttt
     3601 cccgcctgcg gctgctttaa ttgtgttata aggtattctt tgtggttttc cccggaattg
     3661 caaaagtttt tgcaaaaatt taaaattttc acttgctaaa tgataaacat ctatgctata
     3721 atagctttcg tcagtgattc agctgataca atttcatagg ggaatagtac agcggtagtg
     3781 cggcggtctc caaaaccgtt aacgggggtt cgatcccctc ttcccctgct ttcaaaagtc
     3841 gatgttatcg gcttttttta ttttctgttt tggtaacact ttttacctca ccatggagcg
     3901 atctgaagaa tcacgccatg atggggtgtt attttaatgt gaattaacag gaatgttttt
     3961 catcatgata ttgaacatac gttcgattta tgttatactg tatttgtgta atcgaacaga
     4021 aagcggatgc gattttccga ttgttagatg ccctctagtc ttacaaagga gggtgatgcc
     4081 catgaatacg atggaagtac ttactctgtt gttagttgtc tttgcagctt tatcatacat
     4141 tgacagacat ggtgacgata ataacaaaaa gaaatagcat cccctcgcca aagtagatgc
     4201 tatttctttt ctagtatttt actgagggca aatcggaatt ttcacctccg acttactttc
     4261 taaatagatt atacaccaag agggattgag aaatcaatcc ctcttacttt tttatttctc
     4321 aaatctcctc tttctccgca tccggcgcaa tcaccgcccc aatagcggta gcataatcgg
     4381 agtactccgg aatagtgacc ttcacatccc acaggctctt gaagtcttcg atgatctggc
     4441 ggcactcgaa gaatttggtc aggccgccga tgaggatata atccttgatg ccggtgttcc
     4501 gggaggccag gattccggcc tggcagatgt tctcgatgac catatggacg ataccagccg
     4561 ctatgtcttc gggcttggac tgggcatccg ctttgccgaa gttggacgca gtcacgtcca
     4621 ggttcaggcc ggggagcttt tccttggcca gatcgccgat ccgcaggtcg atctttgcca
     4681 catcaccttt ccgggacaga gggacgatct catggatgtc cccggtattc agtatgatcg
     4741 aggacagtcc gcagatggtt ccgccaccga ggccgacacc acctaaatgc ttcatctcat
     4801 tctccgtcac cttgacgaaa aagcttccgg ttcccatact gataacgata acttcctttt
     4861 tatccgtcag ataatagcct ccggttcctg tggcaacgaa ttcatctacc ttataggtcg
     4921 gacgatcgta taccgctttc tccacatatt tgcttcccac gccggtgata tagacacctt
     4981 ccacatctaa aagctgcaga ttattctcat acagatatct tccgaaagca ccgtagaggg
     5041 atgccacctg gctgtctgcc ttgactgcca tgggcggaag catatgttct ccttcaaaag
     5101 ctacgatctt ggtggtactg ctgccgatat caattcctat tcttacggcc atatctttgt
     5161 cctcctcata atgaaatgct aaaaagcttc tttagaacaa tcggtccatg gcatcctgca
     5221 cggagctgac gccgatgaca cggattttgc ttcctttttt gcactcttcc agtgacacgt
     5281 agggcaccac gcattcctcg aagcccagtt tctccgcctc cgccacacgc tgttttgcca
     5341 tgctgaccgc gcggacttcg ccgcttaatc ccacttctcc gaaagctacc attttcggat
     5401 tcaatgcctt attccggaag ctggacagaa tcgccagcac gattcccaga tcaatggccg
     5461 gctcctggat cttgatacct cccgtaatat tcacgtaggc atcgcaggag gacagctgca
     5521 ctcccgatct cttctccaat accgccatca gcaggttcac acggttgaaa tccgtaccgg
     5581 tggtctgtct tctggggatg ccgaaattac tgtggcacac cagtgcctgc aattcgatca
     5641 gaagcggtct ggtgccctcc atggtacacg ccaccacaga gccgctggca ttctccggcc
     5701 gtccgttcag catatattcc gaaggattct tgacctctgc cagtcccgtt tcccgcatct
     5761 cgaaaacacc gatctcgttc gtagatccaa aacggttctt caccccccgc agaatacggt
     5821 aggacgcatg tctgtcccct tcaaaataaa gaacggtatc caccatatgt tccagcactc
     5881 tgggacctgc caccgtacct tccttggtca catggcctac aatgaagatt gagattccca
     5941 gtccctttgc cagctgcagc aaaataccgg tggattcccg cacctgagac acgcttcccg
     6001 gcgccgccga cacgttctcg ttgtacatgg tctgtatgga gtcgatgatc acgacctccg
     6061 gcttgctctt gcggatcact tcactgatga tatccagatt cgtctcacac agtagcagca
     6121 tatgctccga aaaagccccc agacggtccg cccgcatctt gatctgtacc ttggattcct
     6181 caccggaaat atacaatact ttatgtcccg ccgtggataa gttatggcag acctgcaata
     6241 acagtgtgga tttaccgata ccggggtctc cgcctaccag cgtcagagat ccctgcacaa
     6301 tgccgcctcc cagcacccgg tccagttcct taattccggt gagagtctta ttttcctccc
     6361 ggatagcgat ctccgacagc acacagggct ccgaagcctc tctcctgtct ctctttgcac
     6421 cgtttccggt atttttcaat tccgttttat ctatcgtctc ttccacgaaa gtattccact
     6481 ctctgcagcc gggacattgt cccagccatt tcgtggattc atagccacag ttctgacaga
     6541 aaaatacagt ggccgtcttt cctttttcca tgctctctcc tcaatcgttg taatacctat
     6601 tcagcccccg caaaactgcc tccggaaact ctgaatattt aaaaaaagcc cagtcacgaa
     6661 gtactacaat atcctccgtg cctggactcc tttgcagtgc gtccttacgc ctctacgatc
     6721 ttaacaggta cttcacctgc tgcttccagt tctacgctca ggctcagttt gccgccaagt
     6781 ccggttttca tgattcctac gttcttaccg tcgataaata cttcgtactc ggtatcgtcc
     6841 ttcagtccta cagtgatctg ggaatcctcg tcaccttcta cgatgaactc cacactgttg
     6901 tcagcttcct tgaactccag tacactggtt ccgggaacag attcatataa aaacatgccg
     6961 ttcttttcca gctttgtcat ctcattgtag gtcttaacct tcagcagatc tcctgcatgc
     7021 tcataatctt ccacttttgc cttcttggcc agcttgtgat taccaaagct gatagatccg
     7081 tctgcttcac tgcgaagtat ttcttccact actgccatct gtctgtcctc ctgaaagtaa
     7141 ctgtatccgc aatacatctt tctctttcgt tctttgcgaa tggggtttga atagttacag
     7201 tataatatat ctcaccctgt ttttctacaa aaagttttat ttattttgta actattcaga
     7261 gtcatgtaaa ttgtgtcata ggtacgtcga atgcttgcat tcgtaagaac ctttgacgta
     7321 atttatagga cgaagtaacc tcaatgagca aaaggaacgc tctgcaagag cgattttgca
     7381 aattgagtga ctgaatagtt acaaatactt aatttttcac agtaaataca acttttccat
     7441 ccttgaatcc ggcggatacc ttatctcccg gtaccacctt tttctgcagg atctcctctg
     7501 ccagagcatc ctctaccacg gactggatcg cacgcttcaa gggtcttgct cccatcttgt
     7561 tatccgcata tttggatacc agatgctcct tcagcgctgc tgtgatggtc agatggatcc
     7621 ccagctgatc ctcacatctc ttgtacagat tggatgccag aagattcacg atctccttca
     7681 tgttctcatt gttcagctga tggaacacga taatatcatc aatacggttg ataaattccg
     7741 gcttgaagct gcgctttact tcttccatga cattggattt catcttctcg taatcctttg
     7801 tctcgctctt ctctgtggca aagcccagat tcttgggatc cacgatacgc tgtgcacccg
     7861 cattggaagt catgatcagc acagtgttct tgaagctgac cttgcgtccc ttggaatccg
     7921 tgatatgacc gtcatccagt acctgcaaaa gtacattaaa tacatccgga tgcgctttct
     7981 cgatctcgtc aaacagaaca acagagtagg gattacggcg aaccttctcg ctcagctgtc
     8041 cgccttcttc aaagcctaca tatccgggcg gtgatccgat catcttggag accgaatggc
     8101 cttccatgta ttcggacata tcaacacgga tcatggcatc ttcagagccg aacatggctt
     8161 ctgccaatgc cttggacagt tccgtcttac caacaccggt aggacctaag aacaggaagg
     8221 agccgatggg acggttggga tccttcagac ccacacggcc tctgcgcatg gccctggcca
     8281 ctgcggtcac tgcttcctcc tggccgatga cacgcttatg cagcgtcttc tccagtttta
     8341 acagtcgttc gctctctttt tccgccagct tctgtacggg gatcttggtc cactgggcaa
     8401 cgacctcggc gatctcattc tcaccgatac tgataacatt gcccgcttcc tgtttctccc
     8461 gctttttcat caggcggtca tattttttca ccagcgcatc ctgctgcttc ttgatctctg
     8521 ccatctggtc aaatgcttcc atgcggatag ctctctccat ctcctgatcc atctcgtgga
     8581 tacggtcagc gatctccttc gctttatccg gggcatccaa tgcactcagt cttgcgcttg
     8641 ccgaagcttc atcgatcaga tcgatggcct tatccggcag atttctgtca ttgatataac
     8701 ggctggacaa tctgacagca gcctcaatgg cctccggggt gatctgcaca tggtgatgat
     8761 cctcatattt tcctttgatg ccctccagaa tacggaccgc ctcttcctct gtaggttctt
     8821 ccacagtcac cggctggaat ctgcgctcca gagccgcatc cttctcaata tacttgcggt
     8881 attccgcaat ggtggtggca ccaatcagct ggatctctcc acgggccagg gaaggcttca
     8941 tgatattgga agcgtcaatg gcgccttccg cacctccggc accaatgatc gtatgaagtt
     9001 catccaggaa taaaataata ttcccgtcct ccgtcacttc cttcaatacc ttcttgatac
     9061 gttcctcgaa ctcgccacgg tacttacttc ctgccaccat tcccgacaaa tctagtgtca
     9121 gtactctctt attctgcacg gtaaaaggta cgtctcctgc cacaatgcgg gctgccagac
     9181 cttctaccac ggcggtctta cctacaccgg gttctccgac gagacaggga ttgttcttcg
     9241 tgcggcgact taagatctgc actactctgc ggatctcttc ttctctgcct accacaggat
     9301 ccaattttcc ttctctggcc agagccgtca ggtctctgct gtattgttcc agtgtagact
     9361 gcttcgcctt gcggtctccc tttttcccaa gatcttcctt gtacagattg ccgtcctgtc
     9421 cgatggcgat cagggtatct acatagatct tctgcacatt ggctcccaac gtgttcagca
     9481 gacgcacggc aacattctcg ccctctttga tcagtgcaag aagcagatgc tccgtgccgg
     9541 tctgcttctg tccgaaacgg gcagcctggc ggtgtgcctc ttccagaatc cgggcagctc
     9601 tgggagaata tccctctttg tccttcacgg ccacaccgtt ttcaaaggcg atcagatccc
     9661 ggatcatttc catgacctga tccacttcta ctccgttatc cgccaacact tttgcggcta
     9721 caccggtatc ttcctttagg agtcccacca gaatgtgttc ggtcccgata tagccctgtt
     9781 tcaggcctct ggcacatctg ccggcctggg caagcgcatt ctgcgcttta tccgtaaact
     9841 gtgactgcat caattcagtc ctccatattc ttcataaatt tcatcaatca attcatgtac
     9901 ttcctgcctg gaaatatttt tgcagctgct cttagcaagt attacctgca gttccttccg
     9961 caattcatcc accgcacgca tacggtccac atccagagcg atcacggctc cctttcgcct
    10021 gtcgaccttg acatagccct cctgcttcag aaccgtatag gccttattta cagtatgcat
    10081 attgatcccg atggtatccg ccagctgccg tacactcggc agggaatctc cctcatgaaa
    10141 ctgtgacgtg gcaattccca tgatgatctg attgcgcagt tgcatataga gcgcttcatt
    10201 actgttgaaa tctatctcta ttatcatcaa aatcactccc ttcccactgt caccgattgt
    10261 tatacgtcta ttataacagt gagggcggtg ctgtcaactg cagaccggga aagtttatca
    10321 aaattttaaa tcgagtagga ggaatttctc ctaaatgaga aattccgacc tctcacacca
    10381 cc
//
LOCUS       DAWEJW010000098        10206 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2272_length_10206_cov_65.112009, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000098 DAWEJW010000000
VERSION     DAWEJW010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10206)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2272_length_10206_cov_65.112009"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2366 a         2843 c         2136 g         2861 t
ORIGIN      
        1 gaacggaaga tctggctgat atttttgtcc ttccattcct ccggaagtga gagttcagtc
       61 tctacatatc gtcctttctg cacttcatca ggtgacgctg cgatctgctg atcgttctta
      121 ttcccacacc cggtgaacag tcccatgacg atcgccatac aggttgccag tgcgattcct
      181 ttttgcatgt tattgttgtt aaagtagttt ctgtttcgca tcttaatatc cagtcccttc
      241 ccgtaatctg taaatttgcc attccacagc tttttcttac aggaaaagta tagtggtttc
      301 ttttttaaga taacagtagg catggtttaa gatgccatta gcacctcttt aagatttgct
      361 taatttgctc acacaaaaac ggaaccggca caaacgccga ttccgtcttc tccggaaacc
      421 cttttaggaa ttcaatatct tcataaattc ttctccggta atggtctctt tttcatataa
      481 atactgagat agttcatcca gtttagcacg gttttccagc aggatctgcc ctgccttctc
      541 atgctgcttc ttcaccagtt ccaccacttc cttatcgatg gcagtctggg tctccatgga
      601 acaggtcagg gaggaatctc cacccagata ctggttcgtc acggtctcca tggctaccat
      661 gtcaaaatcc ttgctcatac cgtagcgggt gatcatacct ttggcaagct tggtagcctg
      721 ttcgatatca ttggaagctc cggtggtcac agatccgaag gcgatctctt ccgcggcacg
      781 tccgccggtc agtgtagcga tcttgttctc catctcttcc ttactcatca gataatgatt
      841 gccttcctct tccacctgca tggtataacc cagtgcaccg gaggtacggg gcacaatggt
      901 gatcttctgc accggtgcgg aattagtctg cttcgctgct accagtgcgt gtccaatctc
      961 atggtatgcc acgatccgct tttccttatc tgtaaggatg gcattctttt tctgataacc
     1021 ggcaatgact acttcaatac tctcttctaa gtctgcctgg gttgcgaact tacgtccatc
     1081 tctgactgcc cgaagagctg cctcattgac gatattggca agctctgcac cggaggctcc
     1141 ggaagccata cgggcgattt tattgaaatc tacattatct gcgactttga tcttacgggc
     1201 atgcactttc aggatttctt ctcttccttt caggtcggga agctctaccg gcacacgtct
     1261 gtcaaaacgt ccgggacgta acagtgcggg atccagactc tccggacggt tggtggccgc
     1321 caggatgata acaccgttgt tgccttcgaa tccatccatc tcggtcaaca gctggttcaa
     1381 tgtctgttct ctctcatcgt ttccgcccac ctgtccgtca cgctttttac cgatggcatc
     1441 aatctcatcg ataaatacga tacagggagc cttttccttc gcctgtttga acaggtctct
     1501 gaccttggac gcacccatac ctacgaacat ctccacgaat tccgaaccgg acatggagaa
     1561 aaagggaaca ttggcttctc cggctactgc cttggccagc atcgtcttac cggttccggg
     1621 agggcctact aagaggatac ccttcggcat ggaagcaccg atctctttgt atttgtcggg
     1681 atcatgcagg tattccacga tttccgtcag attttccttc gcctcgtcct ctcctgccac
     1741 atcggtgaac ttgataccgt tggaagactt cacatagacc ttcgcattgc tcttgcccat
     1801 gcctcccatg ccgaacatca tggaatcgcc gccggccttt ttcgccatct gacggtacat
     1861 caattgtccg aagccaataa tgatgatcaa aggcagcagc cagctgatca gaaagcttaa
     1921 aaccggtgat gcttcctcca cgatctcact ggagaatttt gccccactgt catacagtct
     1981 ctgcgttctg tccggatcat ccatcagacc ggtcttgtag atggtcttgt catcttttgc
     2041 cgtgaatacg atctgattgt cctggacctg tacctcatcg atctgtttat cataagtcat
     2101 ctgcataaaa gtgttgtaat ccacttcctt cactgccctc tgtacgatct gaggtaccag
     2161 aagactgttg atcaggataa tcacgatcaa tgcaatcata taatagaata tgaacggttt
     2221 tttgtttggt tgtttgactt cctgcataca ttttcccttt ctcattcctg tattattgtc
     2281 caaatctgtt gtaataaatc aatcatagcc actttttgcc acacaatcaa tggatattct
     2341 cttaccttat tctaaaaaaa agataaactt atgcggtgaa aagactacgt ttttcaaaat
     2401 ctgtttcttt ccattttcag gcaaaagaga gacaattaaa atagattttt acttgccaaa
     2461 aggttcaaaa aggaggaaac gtctgtgtcg gatactacca aaaagagatt aaaattcgca
     2521 ggaaagcttc tgtttgtctg tctgttatta ggtatcctgt attacagctt ccgggattcc
     2581 atgggagata tgctgacgga gcttgccaat gtctccccgc aagtggtcat gtccctgttc
     2641 ctgaccgtga tcctctacca tatctttgaa gggcacatca cctggctgat cgtgcataaa
     2701 acgcacccgg aatatccccg cctgaaggga ttctgtaatg ctttctactg ctctttctat
     2761 aagacagcca ctctgggcag cggttccggc atcgcaggaa tctattacct gaacaaagcc
     2821 ggtatcccgg ttcccaatgc caccggcatg tactttttcc aatatattgt ccacaaggtc
     2881 tctatggcag tctatagtct gctattgttt ttggcaacct atggatttat tcatgcttca
     2941 tttgccgatt atcaatgtta tatcctgctg ggatttgcag gagtctgtgt gattgcagga
     3001 gttctgttag ctgtcgctac cgtaccctgg atgcagactg cctgtaatct gctggccaac
     3061 cggttccgtg caaagtatcc ctcctgggag gaaaaactca ccggtgccgg gcataagctt
     3121 gccctgctac aggcggaaag ccggtctctg ttacaggatc caatattgct gcttcacctg
     3181 ttcctgtgta atgtattaaa gttcactacc tggtatatca tcccctggat cgtgttctgc
     3241 aattctcttg ggggagaata cggcgatctg cctttttctt tcctgcaatg ttttgctctg
     3301 acctctctca gccagtcgct ggcgggtgtc atccccaccc cggcggggat cggatccgta
     3361 gaggctgtct ttacattgct gttccggcgt ctcctgccgg aggccaaagc cctatctgcc
     3421 gtgcttctct accgtttcgc caccatgctg ttaccttgtg cgatcggtgc gttctttgta
     3481 ctgggggaga ggattatttc tctgcacagg aaaaagagaa cggcggacta atatgctttc
     3541 ctaaaatctc cacacaaaac aagccacaac acagtattct gtgtcgtggc tcttattatg
     3601 ttccgttatg ataaaaccaa atactaagta ttactttctc tgtaagaaat caggaatctt
     3661 cagagatttc tcttctacgg agcttctaat atctgcaggc ttatgaatac cgggcaccgg
     3721 ctgtatgggt ctcggagcct gtcctccctg tccctgggat gtcgtaggag cggtagactg
     3781 tatattcatt cccttcagag aactgggagt catatgggaa gcaggctgtc tgaatgcagc
     3841 gcctcctccc agacgattct gtacaccgtt gttggccttg tcttccagac cggtagcaat
     3901 aaccgtaatg gtgcaactgt cggacttgga ctcatcatac atggcaccga agataatatt
     3961 tacatcgtct cctgccagct cctgaacata gcttgccgca tcggaagcat ctgccagggt
     4021 aatgtcgccg gaaatattga tgataacatg ggtagcaccg gaaatcgtag tctctaacag
     4081 agggctctcc actgccatct tgacagcttc catagccttg tcatcgccct tgccttcacc
     4141 gataccgatg tgagcaatac ccttgtcctt cataaccgtc tgtacatctg cgaaatccag
     4201 attgataact gcgggaacat tgatcaggtc agtgattccc tgtactgcct gctgcagtac
     4261 ttcatccgcc ttcttcagtg cttccggcat agtggtacgt ctgtccacga tctccagaag
     4321 cttatcatta gggattacga tcagggtatc cacgtgttcc ttgatacgct cgataccgtt
     4381 taatgcattg accatacggg cttttgcttc aaaacggaaa ggcttcgtta ccacaccaac
     4441 ggttaagatg cccatatcct ttgcaagctt ggccactacc ggagcagctc cggttccggt
     4501 accgccgccc ataccacagg tgacgaatac catgtctgca cccttcagag ctgcggaaat
     4561 ctcttctgca ctctcctcgg ctgctttctc accgatctca ggcttggctc ccgcaccaag
     4621 acccttggtc agcttctcac cgatctgtaa cagtttcgga gctttgcaga gctgcaatgc
     4681 ctgcttatcg gtattcaccc cgataaaatc cactccgtca atcttctcat ctatcattct
     4741 atttacagcg ttgttacccg cgccacctac accgactacg atgatcttgg cagctacatc
     4801 cgcgtcattc gtcttaatct ccagcaaggt tagttcctcc ttttaacctt tttcaacttc
     4861 acatagacta tcatataatc attttacaaa tttagcaacc tattttttca aaatgtttca
     4921 caaatgtttc acgcgtccgg tttgaaggta aatttaccct ttccttcctc gtagttttcc
     4981 atttgcaggg ttccgctttt accctgtaac ttctccagga agtccggcaa caactgcagc
     5041 ttgtcctcta tatggctgtt gtcactgccc atggacacct tgatctgtcc gaagtagatc
     5101 gtaatgtcac cggaagaatg cagatagatc ttgtccgcag ccagtgaata tttgttcaaa
     5161 agctttgtca gatccatgat cctggaaaac acctgcggat cctccaccgg cagctgctct
     5221 cccagcacca catggtcgaa ggaaagcccc gtgatctgcg gtattccctg ggtgcggatc
     5281 ccggaacttt ccaccacata gccgtcttta tcaaaataca tataagtatc cagatatttc
     5341 acatatcccg ccagtgcttt ttcatataca gtgatcttga tcgtgtcggg tgccagaatc
     5401 gatacattca tcacatccac aaagggaatg tcctgaatcc ctctgtcacg gtatttcaga
     5461 gacagataca gggaattatc tcccaaaggt cccgacatca cgatctccat gatctcgtcc
     5521 tcggtataat gcacattgcc ctccacatac acggtacgta ccgtatacac ggagtgcaat
     5581 gccaccgcac caccgcccag tgccagcagc aggatgccta tgatcactat tcttctgata
     5641 attttgcttc ggtctctata cactgatgat ccgtgctcct aattctctca agtctctgca
     5701 gatattttca tatccgcgtt ctataaagtg ctgtcctgtc acgaaactct cgccttctgc
     5761 cgcaagcccc gccagaacca gtgccgcacc gccgcgaagt tcacaggctt ccatccggct
     5821 tccccgtaaa tgctccactc cggtgatcac ggcatgtctt ccgtccttca gaacaatctc
     5881 tgctcccatg ccctttagcg gttccgcaat acgaaaacga ttctcgaaaa tattctcttc
     5941 aatgcagctg catccttccg ctttcgtcag caccgccatt gccaccgact gcagatccgt
     6001 gggaaacccc gggtaaggtc cggtccgtaa atacggtatg gcctgcggtc ttttggaaca
     6061 ctgtacgaac agaccttcct ccgaggtgat acactgtgca cccatttttt ccgccacaaa
     6121 ggtcaccgct tccatctgct cccagggagc ctctgtcaaa agtacactgc ctccacagcc
     6181 gatacaggcg aacagatatg tcccggctac aatccggtct gacggaactg tgtaccgtgt
     6241 actatgaagt ggtctgtcac catgaaccag aagttccgat cctccaatcc cctcaatctg
     6301 tgcaccacat gccgtcagaa attcgcacaa tgccgtcact tccggttctc tggcggcccc
     6361 cagtatcctg gtgtctccat ccgccttcac cgcagccagc aaaatattct ccgtagctcc
     6421 cacactggga aactccagtg tgatctctgc cccgtgcatt ttcccatcct tcacctgtgc
     6481 agacagaagc ccttcttcct cccggaattc gacgcccatc tgctgtagtg ccctgatgtg
     6541 cagatcgatc ggccgttctc cgatgacgca cccacccgga tgctccatgg tcacataacc
     6601 acatcttccc agcaatgctc ccaacaggca cagagatgaa cgcattccgg tgatagcttc
     6661 cccctgcatc cgtctggcct ccgctctctc gcagggaagt tcttcatttt ctcccggctg
     6721 tacccggatc cctttttcct gccagtgcac cagacatccc agttcccgaa gcagatgcac
     6781 aaaacgatgt acatctgcaa tacgtgggca gttctgaata taggatactt cattggtaag
     6841 aagcgttgcc gccaggatcg gaagtgccgc attttttgat ccctgtatgc gtaccttccc
     6901 ctgcagcgga atgcctccct gcatgcggat tcctttcata cagcaccatc cccgttttta
     6961 tgataatctt atcttatgta ggaaagcact gtttcgtacc atgtcttctg aatatgtatt
     7021 tttctacagc tcttttaact ggatcctgcg cgccacgctt aataccagtc cgatctccga
     7081 cagcaaaaac aatgccgaag aaccaccgta actgataaac ggcagggaaa ttcctgtgtt
     7141 gggaatggag ttggtaacta cggcaatgtt taagatcgcc tggattgcga tatgtcccat
     7201 aacgcccacc accagcatgg cgccgaacag gtccggtgca ttgttggcaa tgaccatcat
     7261 tcgccataag agcataatga acatgagaat aatggcaacc gctccgaaca atcccagttc
     7321 ctcgcagata atggagaaga tcatgtcgtt ctgtgcctcg ggcaggaagc ttaacttctg
     7381 catactctgc cccagtccct tgccccagat gccaccgctt ccgatggcat acagtgcctg
     7441 cagggtctgg aagcctttat cctgtgcctg actttccggg ttcagccacg cctggattct
     7501 tgccagacgg aacttgccac cgatcttatc tcccaactgt acgactgcga ctaccaaaag
     7561 tccagccgcc gccagtacgc tgcctcccat gatgatgaat ttcttgtaat ccgggcttgc
     7621 cacgaatacc atcagcaccg caatacccat aataataatg gcggaactca aattatccgt
     7681 aatgaggtac accgatgctg cgatcggcag gggtgccgcc atcatggtaa agaagccttt
     7741 catggtacgg acgctttttc ccatcttgca taccagactt gccaggaaca ggatcataca
     7801 caatttcgct acttccgcag gctgcaggtt gaagcccgta tgtgggatac cgatccatct
     7861 ggtagcgccg tgggaagtga tacccagggg ggtctttacc aggaagatca ggatcatgga
     7921 caccacatat cccagggtgg caaaccgttc ccagaaggta tacgggatat tggcaatgac
     7981 catcatgaag accagaccga tgatattggc gatcagctgt ttcttcatat aataggcggc
     8041 atctccatag gaatcataag cctcatagga gctggccgaa tagatcatga tcagtccgaa
     8101 tcccagtaaa aacagcacaa taaaaagcag gctgtagtca aaaaagtatt cggattgttg
     8161 tttcttttta cgtcttcttc cccgcgtttc cgccatgcct gcttttactc cttacttctg
     8221 ttatgatttc ttattccgcc agtccgtgta catattcctt gaaaagctgt cctctgactt
     8281 catagttcgg gaacatgccc cagcttgcac aggccggaga caacagtacc gcttctcccg
     8341 gctgggccag ttcggtacac agcttcaggc attcctcata ggtatctgcg aaacggatct
     8401 tatcaaagcc atgctttctg gcacactcgg caatcttctc tctggtctgg ccgatgagga
     8461 caaggcactt taccttgcca tcaaagctgt cgatccactc atcgtactcg ctctgcttgt
     8521 catagcctcc gcctatcagc acggtaggct tgctcatggc tttgatgccc tggatcgccg
     8581 catcgggatt ggttcccttg gaatcgttgt aatagtccac accgcccttg gtagccacga
     8641 attcgatccg gtgctccacc gcatggaaac cgcggatcac agtaaggatc gtctccatgg
     8701 gcacctgcat accttctgca atggctatgg ctgccatgac attttccaca ttgcacaggc
     8761 ctaccagatt catatccttg tgaatatcca tcagttcggt ctcttcgccg ttcacactct
     8821 tgataatctt gtctccccgc aggaagtaac cgtttttcag ctcgtgatgg gaggagaaca
     8881 gtaccggagt cgcaggacat cttcctgcga aatctctggt ataatcattg tcataattca
     8941 atacacagat atcatttttt gtctggtggg aagccactgc ttccttggct gccacatagg
     9001 cctccatggt atgatgtctg ttcaggtgat ccggtgtaat attcaggatg gctgatacta
     9061 ccggatggaa tgtatgtatg gtctccagct ggaaagaact gatctctccc acggtgacac
     9121 tatcctttga cgtcttcgtt acttccagtg tataagggtt gccaatatta cccactacaa
     9181 aggtcttctc cgcaccgaaa tgagctttca tgatctcacc ggtcaatgtg gtggtagtag
     9241 tcttgccgtt agtgccggta atggcgatca ctctgccctg ttccgcaagg aatcccagtt
     9301 cgatctctcc caggatctgt accccgcggt ccctcatacg gttcaccaga ggaatatccg
     9361 taggtactcc ggggcttaaa acggctgcct gaatgctcag ttctacctcc tccggcagtt
     9421 ctccggcgat acagactgcc ttgctttccc cgggaagctt cgcctccaga tcttcctttg
     9481 tcaatttatc actgctgtca tacagaacca cgtctgcatg gtatttttcc aataaagcca
     9541 ctgcaccgat cccgctgatg ccggagccga tgaccagaca tttttgtcct tctaacattt
     9601 tccttaccat acctttctgt aaaagtccat tagttcatta taacgcattc cctacaaatg
     9661 gggaagagat ttttacagga gacttacgca aagaagccct ccaacatata tatgtcggag
     9721 agctccggtt cattccagtt tctgtgtaca ataatcttcg gttctacgct tctacgatca
     9781 gatatctgcc gtctccgata tcgaatactt catctgcctc cagaatctca tcatccacag
     9841 cgccgtccac atccatgccg gcctcatcaa aatactcaat gacatcctcc acggagtcta
     9901 ccacgactgc catgcactct tccagaaaat cttctgcctc atccagtgtc tctgctacag
     9961 cttccggaaa aagctggagc tgtttgtcca ggaagcattc cagtaccgca tcatcatact
    10021 ctcgcatgct attacctcca aattcttaca aaaatgtctt atttattttc ataataatat
    10081 ggatataaga ttacgtcaat tagcaattcg atatagatgt aaattgagag ttagtaaaga
    10141 aatgctatca aatccccgta gcccttggca gggtataaga aacgcggcac gctcgtactt
    10201 tagtac
//
LOCUS       DAWEJW010000099        10149 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2288_length_10149_cov_55.763919, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000099 DAWEJW010000000
VERSION     DAWEJW010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10149)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2288_length_10149_cov_55.763919"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2988 a         2234 c         2749 g         2178 t
ORIGIN      
        1 ctggaatacg ctcccggcag cgagaaacac atggtagaat atctccagaa aggccatgca
       61 caggatctga caactatggt gcctaccgga agggtgttcg accggtaata acttttcctg
      121 cgatagaagc ggcggggatg atcccgactt ccgcaaagcg gctgtcaatg ctgtggctgc
      181 ggttgtcacc caggacaaaa tattctccgg gtgcaagcgt caggggagcg gcggctttgc
      241 cggcatcctc catgagagaa aagccatcgg gggtcggttg gggaactcca ttgacataga
      301 ggattccctc tgtgatctgt atcgtatctc ccggcagggc cacgatccgt ttgacgatat
      361 cgcgtcccag cgtctcgtgg cggatgacaa cgacatcgtt atagtccaga cagtctggca
      421 gaccgaattt ctgaaggagg accggctgcc cggaggtgta ggtcggctcc atggaagaac
      481 cgttcacaaa atacagctgt accacgaaat gggacaccag ataacagata cagccgataa
      541 gcaatacaaa aagcagataa gaacgggtgc gatgattcat aagggatcct ttccggtttg
      601 ttaaaatgct gataaagata tcaaaatcta tcagatagca taccacattt cgggtggcaa
      661 taacagagaa gagaaacaat aagagaaaaa gaggtaaaag caatgaaagt attactggta
      721 aacggaagcg gacatgcgaa gggctgtacc tacacagctc tggaggaagt agcgggagca
      781 ctggagaaaa acggtattga gacggagatc atccaggtgg gtacacagcc catcgccgga
      841 tgtatcggct gcaatgcctg tctgaagaac ggaaagtgtt tccgggagga tggcgtgaat
      901 gaatttgtgg aaaaagcgaa gactgcagac ggatttgtct tcggatctcc ggtgcattat
      961 gcatctgctt ccggtatgat cacttccttc ctggacagag cattttacgg caagagcgcc
     1021 ctgttccagg gcaagcccgg tgcctgcatc gtcagctgca gacgcggtgg ggcttccgca
     1081 gcctttgacc agttgaacaa atattttacc atcaactgta tgccggtggt atccagccag
     1141 tattggaatc aggtacacgg caatactccc gaagaggtaa aacaggatct ggaaggcatg
     1201 cagaccatgc gtaccctggg taacaatatg gcatggctgc tcaaatgcat tgccgcagga
     1261 aaagctgcgg gcgtggattt cccggagaga gaaccggcgc agaagaccaa ctttattcga
     1321 taaggagaca gatgaattat ggcagagtgg aatggattgg gaagatatcc ggaggatgcg
     1381 gatgtgaatg atcgtccgga agagggtacc ttgctagcct atgtggacgg aagctatcag
     1441 gatgcactga aaaaatacgg tttcggctgt atttttattc ttcccgacgg acgcatctat
     1501 acagaatacg gtaatggtga caatccggac tctctgaaac agcggaatgt ctccggagag
     1561 atgctgggag ccatgtatgc ggtacggttt gccttaaaca gcggctttac agtcatcgag
     1621 atccgctacg actatgaagg aatcgagaaa tgggtcaccg gtgcctggaa aagtaagaat
     1681 gatctgacga aaaaatatgc acagaccatg ctgggctggg gacagaagat tcagatccgc
     1741 tttaccaaag taccggctca ttcgaaggta aaatacaacg agctggcaga tgagaccgca
     1801 aagctgggtg tggcaaacgg aaacggtatt cccaaagtca aaagtctcag tgacttcgaa
     1861 gcagcagacg gtgtggaagc gtgaaaacat atacttgcgg ccgtgtgtct gtagcccgga
     1921 agttaagggc ttcggaaagg aatgaggaat agaatgcgtc tgaataaata tctggcacag
     1981 tgcggtgtat gttcccgaag agatgcggat aagctgatcg aacagggaaa agtactggtc
     2041 aacggtcagg tggccggaat gggacaacag gtggaagaaa cggacaccgt gcaggtgggt
     2101 aagaagattc tgcagggaaa acagacacag aaggtactgg cattttacaa gccggtggga
     2161 gtgacctgca cggagagaga tcctcatgca gagaaaaaga tcatggatat gatccgctat
     2221 ccggaacggc tgacctatgc gggacgtctg gacaaagatt ccgaaggcct gatcctgctc
     2281 accaatgatg gagatctgat caatgccatg atgcggggcg ccaaccgtca tgaaaaagaa
     2341 tatatcgtaa agacagacaa ggaaataaca cccgattttt tggagaaaat ggcgaaaggt
     2401 atttatctga aagatctgga catcactacc agagaatgtg agattgaaca gatcggaaaa
     2461 tttaccttcc gtatcgtctt gactcaggga gtcaacagac agatccggag gatgtgtgaa
     2521 gcctgtggat accgggtaaa agcactgaaa agaatccgga tcatgaacat tctgctgggg
     2581 gatctgaagc cgggaaagtg gcgtgaggtc atcggagaag agctggaaca gcttcaaaga
     2641 gacaccggaa tgttaaatcg caattaaaat atataaaggc aggaatacaa gatgcagtct
     2701 tccaaaatag accggatcaa agatctggta gaacaactga atgcagcttc cgaaagctat
     2761 tacgccaagg atcaggagat catgagcaac tatgaatacg acaagctcta tgatgaactg
     2821 gtggaactgg aaaaagagac aggcgttact ctggccaaca gtcctacggt aaacgtaggc
     2881 tacgaagcgg tggatgagct tcccaaggag cgtcacgaga gccccatgct gtctctgggc
     2941 aagacgaaaa gccgggaaga actgcgggaa tggctgcaag gcaatcctgc aattctcagc
     3001 tggaaactgg acggtctgac cattgtattg acgtatcgtg aaggaaaact tgcaaaagcg
     3061 gttacccgtg gcaacggcga gatcggtgag gtcattacaa ataacgcccg tacttttaag
     3121 aatcttccgc tgaatatttc ctataccgga gaactgatcc tgcggggaga agcggtgatc
     3181 acctacagtg attttgagaa gatcaacggg gagatcgcag atgcagaggc aaaatacaag
     3241 aatccccgaa atctgtgcag cggctctgta aggcagctca acaatgagat cacggcaaga
     3301 cgaaatgtcc gcttttttgc ttttacgctg gtaaaggcgg agggtgtgga ttttcatgat
     3361 tccaaggaag cccagtttgc ttttttgcag gagcagggct ttgcagtggt ggaacatgtg
     3421 gcagtaacgg aagagaacat tttatcggcc attgaagatt ttgaacacaa gatcgagcac
     3481 tacgatatcc cttccgacgg cctggtactt acttatgaaa gtatcagcta cggacagtcc
     3541 ctgggcagaa ccgcgaagtt ccccagagat tccattgctt tcaaatgggc ggatgaactg
     3601 cgggagacta cattaaaaga ggtcgagtgg agcgccagcc gtaccggtct gatcaatccg
     3661 gtagctattt ttgagccggt ggaactggaa ggcacaacgg tgagccgcgc ttccgtgcat
     3721 aatatcagta tcatgagatc cctcagactg ggaatcggag accatatcac ggtctataag
     3781 gccaacatga tcattcccca gatcgctgag aatctgacag gcagtgataa tgtggagatt
     3841 ccaaaggtgt gtccggtgtg cggacagccc acggagatcc gacagatgaa tgaagtacag
     3901 tctctgtact gcaccaatga gaaatgcccg gccaaggaga tcaaatccta cactttgttc
     3961 gtcagcagag atgcgctgaa tattgacgga ctctccgagg caacattgga gaagctgatc
     4021 gatcagggct ttatccatga atatgcggat ctgttccggc tggaccgtta caaggaggtc
     4081 attaccggaa tggaaggatt cggagaaaaa tcctatcaga atatgatgga cagtatcgag
     4141 actgcaagac ataccacgct gccccggttg atctatggac tggggatcgc aggaatcggc
     4201 gtggccaatg ccaaggtgct gtgccggtat tttgattacg atctggaacg aatgcaggca
     4261 gcggacgaag aaacactgag tgccattccc ggtgtgggag aggtgctggc atctacattt
     4321 acggattata tgcgggatgc ggaaaatctg gagaaaatag ctcatctgaa ggaaatcctg
     4381 acgatagaga caccacagat cgatgagagt gcacagacac tggcaggaat gtccttcgtg
     4441 gtgacgggaa gcctgaacca ttatgccagc cggaatgatc tgaaagaggt cattgaggaa
     4501 aaaggcggca aggtgaccgg aagtgtcacc ggcaagacca cctgcctgat caacaatgat
     4561 attacctcta catcatcgaa aaataaaaaa gcaaaagaat tacaggttcc cattctgaca
     4621 gaagaggaat ttctgagaac ctataatatt ccttacgagg aataagcaag aaagacccgg
     4681 aggaaaataa aatgcctatc aaaacacaaa atgatctgcc tgccaaagag atactggaaa
     4741 atgaaaatat attcgtcatg gacgaatttc gtgcggtaca tcaggacatg cgtcccctgc
     4801 aggtactgat cctgaacaat atgccggtaa agcaggatac ggagctgcag ctgctgcggg
     4861 cgttgtccaa tacaccgctg caggtggatg tcactttcat gaacaccaag acccacgtgt
     4921 cactgaatac cccggccagc catctgaaca aattttacac tacctttgat gagatcaggg
     4981 atcgtaaatt cgatggactg atcgttaccg gagcaccggt ggaggatatt accttcgagg
     5041 aagtggatta ctgggaggaa acctgtcaga tcctggactg ggcggagacc catgtgacct
     5101 ccaccttaca tatctgctgg gcggcacagg ccggatttta tcattattac ggaatcaaca
     5161 agagacaatt gtcccagaag ctgttcggcg tctatgcaca taaagtatcc aaccggaaag
     5221 ttccgttggt ccggggattt gatgatattt tcatggctcc gcacagtcgt cataccgaga
     5281 caccatcgga ggccattcat gcctgtaagg atctgacgat cctggcagaa tcggagaagg
     5341 ccggcgtatt cctggcaatg gcggaagagg gcaaaaagat ttttgtagcg ggccatcccg
     5401 aatatgaccg ttatacactg aacaacgaat atcatagaga tctggacaaa ggactgccca
     5461 ttcagatccc ctataattat tacccggacg atgacccgga gcagagacct ctgttacagt
     5521 ggagatctca ttgtaacaac ctttacagca actggctcaa ttattatgtg taccagacaa
     5581 caccatatga cttaacggaa attcgataat ttcgatagaa acaggacgat ggtcatggag
     5641 aatggaaaaa agaaaggaag cagaagatga atcagacact gacaagaaaa caatttgata
     5701 ttttatcgat actggcagag gaaaaaggta ctttatccca gagacagctg ggagaaaaga
     5761 gcggacactc tctgggaacg gtcaaccgtg tcatgcagga actgaccgaa ttacagtatg
     5821 tgacagaggg agagatcaca ggtgccggaa tctccgcact ggagccttac cgtgcaaagc
     5881 gcgccatctt tattgcagct ggattcggca gccggctggt gcccattacc tttaacaccc
     5941 cgaaaccact ggtacgtgtg catggacagc gtattattga cggactgatc gatgcctgtc
     6001 gggatgccgg gattaatgaa atctatattg tccgggggta tctggcagag cagtttgatc
     6061 agctgctgta caaatatccc atgatcagat tcctggaaaa tccggtttac aatgaagcta
     6121 acaatatttc ctccgccatg gtggcaagat atatgctttc caatgcctat gtatttgagg
     6181 cggatctgct gatctccaat ccgcagatca ttaaaaaata tcattatacc tccgattttc
     6241 tggcaataaa aaaggacaga accgatgact ggtgcttcac ggtaaaagac ggagtcattg
     6301 tagaagagaa agtcggcggc ctggattgct ggcagatggt gggcatctct tactggaacg
     6361 aagaagacgg acataagctg tcagatgata tcaagatgac ctacgaacag cccggcggca
     6421 aggagcgtta ctgggaacag gtacccctgg tattctgcca gaagcattat aaggtagaag
     6481 tacgtgaatg ccgggaaaat gacattatcg agatcgatac tttccgggag ctgaaagcca
     6541 tcgacaagac gtatgatgtt taaagaaaaa taagtattgc tattttgtgc gaatggtatt
     6601 attatatgtt catatttaac acaaagtgtc tgcgaatgga acgcagacat tttgtgtact
     6661 tattttagcc gaaaacctat gaaaaccgtt caaagaatta aaaaatatca tacataaatg
     6721 aacgattgga aaaagaacgc gaacaaaaaa aggaggaatc ccataatgga gcagttgata
     6781 caaaaacccg tcaaaagaaa tattttattg aatcccggac cgtctaccac cacagatacc
     6841 gtaaaatatg cacaggtcgt gccggacatc tgtcccagag aaaaagaatt cggcggactg
     6901 atgaagggac tcagagaaga cctggtgaaa atcgtgcatg gggatttgga aaagtatacc
     6961 agtgtcctgt tctgtggatc cggcaccatc aatattgatg cctgcatcag ttctctgctg
     7021 ccggagggcg gcaaagtact ggtggtggat aacggagcct acagcaccag agctgtggaa
     7081 gtatgccagt attacggact tccctatatt gatctgaaat tcccggtgga tgaactgcca
     7141 gacttagaaa aagtggagca gaccctgagg gagaaccctg acattgccct ggtacatacc
     7201 actcataatg agaccggtac cggaatcctg aatcccatcc gcgagatcgg agcccttgct
     7261 cataaatatc atgctacctt taccgtggat accacatcca cctacgctat gcgtcccatt
     7321 aacatcgagc aggataacat cgacttctgc atggcatccg cacagaaggg tctcatggcc
     7381 atgacgggac tatcctttgt ggtaggcagc cgggcaattc tggaggcttc gaaggaatat
     7441 ccgaaacgtt cctattactg caacctgtat caacagtatg atttcttcca gaggaccgga
     7501 gaaatgcatt ttaccccgcc ggtacagacc atttacgcca cgatccaggc attgaaggaa
     7561 tactgggcgg agggcgagga agccaagtgg gcgagacata cgagagtctt tgaggccatt
     7621 catgaaggac tggatgcact gggattccac gatgtgatcc gcagagaatg gcagtcggga
     7681 ctggttgtgt ctgtgaagta tcccgatgat ccaaactgga attttgaaaa agtacatgat
     7741 tattgctatg agcggggatt taccatttac cccggcaaaa tctctactac caataccttc
     7801 cgcctgtgtg cactgggtgc gattgacaga ccggatatcg aagccttttt caaagtgttc
     7861 agagaagcac tggaagtcaa taaaattcag attccggcaa aatataagga ggcataggat
     7921 caaatgaagc gtgtatatac ctgtttctgt acagatgtca ttcatgaggg gcaccgcaat
     7981 atcatcagag aagccggaaa atacggcgag ctgaccatcg gcgtcttaag tgatgcggca
     8041 atgatccgtt ttaaccgttt tcccaccatc agcttcgagg agcgtatgca gttagtaaaa
     8101 gatatcccgg aagtcagcaa tgtggtcgtg caggatgatg taatgtatga taaggtcatt
     8161 gaagaattgc gccccgacta cgtgatccat ggggataact ggcaggaggg tgctttgaag
     8221 gctatccgtg acaacgtgga aggactgctg aagacatacg gcggtgagat catagatgtc
     8281 ccttatacat ataatgaaga agtaaaaaga attgatacga ggatcaagga aaagctggcc
     8341 atgccggaat accgcagaaa gcgcctgcgc cagctcattg agatccgtcc cattgtcaaa
     8401 gcactggagg ttcacagcgg cctgaccgga ctgatcgcag agaagaccat cgtggagcat
     8461 gatggtgagc tggaccagtt cgacgccatg tggatcagct ctctgtgcga ttccacggcg
     8521 aagggcaagc cggatatcga gctagtggac atgacttccc gtttccgtac cattgatgac
     8581 gtgacggaag tgaccacgaa gcccattatt tttgacggag ataccggcgg actgacggag
     8641 catttcgtat acacagtgag aacactggaa aaaatgggtg tttccgccat cattattgag
     8701 gacaagaccg gacttaagaa aaattccctc ttcggaaacg atgtggtgca gactcaggac
     8761 agcatcgaga acttcagtgc caagatcgca gccggcaaaa aggcacagct gacggatgat
     8821 ttcatgatca ttgccagaat cgaaagcctg atcctggagc gaggcatgga agatgcactg
     8881 aaccgtgcca gagctttcgt ggcagcggga gcggatggca tcatgatcca cagccgtaag
     8941 aaatccccgg atgagattct ggaattctgt gataaattcc gggcagagga tccgaagaca
     9001 cctatcgtgg tggtgccgtc ttcctttaac agcattacgg aaaacgaact ggccgcccac
     9061 ggcgtcaata tcgtgatcta tgcgaaccag ctgaccagaa gtgccttccc cgccatgcag
     9121 cagaccgccg aggacatcct gcggtatcac agggcacagg aagtggacag cagactgatg
     9181 ccgatcaaag atattataac tttaatagac gaaatttaac tgacaggggc aggaaaggaa
     9241 tacttacatg aaagcagaaa actttgcgaa aattttaaat gcggactttt ataccggggt
     9301 gccggattcc cagttgaagg ccctctgcaa ttacctgatg cacacctatg ggatcgatcc
     9361 gaagcatcat gtgatcgcgg cgaacgaagg caattgcacg gcactggccg ccggctatca
     9421 tctggctacc ggcaaagtcc cggtagtata catgcagaac agcggagaag gcaatatcat
     9481 caaccccgtg gcatccctgc tgaacgataa agtctatgcc atccccatga tcttcgtcat
     9541 cggctggcgc ggtgagccgg ggatccacga cgaaccccag catatctacc agggtgaagt
     9601 gaccctgaaa ttgctggagg atatgggaat ccgctatttt gtcatcggaa aagacacaac
     9661 ggaagaggaa gtaagtgctg ccatgcagga gttccgcaag gagctggatc gtggtgagga
     9721 tgtagcgttc gtgatccgga agggtgccct cagttatgag gaaaaggtgg agtaccgcaa
     9781 cgacaatccc atgatccggg aagagatcat acgccacatc gtgcaggctt ccggcgagga
     9841 tcccattgtc agcaccacgg gcaaagccag cagagaactg ttcgagatca gagaacacaa
     9901 cggacagagc cataagtatg atttcctgac cgtaggttcc atgggacaca gcagcagtat
     9961 cgccctgggt gtggcagtac agaagcccgg tacgaaggta tggtgcgtgg acggtgacgg
    10021 cgcagtgctg atgcacatgg gttccatggc ggtactgggc agtacggcac ctgaaaatat
    10081 ggtacacatc gtcatcaata acggtgccca tgagaccgtg ggcggtatgc ctaccgtggc
    10141 agccgatat
//
LOCUS       DAWEJW010000100        10017 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2325_length_10017_cov_67.670749, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000100 DAWEJW010000000
VERSION     DAWEJW010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10017)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10017
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2325_length_10017_cov_67.670749"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4191..4290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2422 a         2684 c         2036 g         2775 t
ORIGIN      
        1 agattcttgt gcaagcacaa atctgtactt ggctcatcgt atacacaaaa aaggaccggc
       61 actatttcag taccagtcct gtctccagat atacgggaag tgcccgtaca tataccatat
      121 ctattgtgcc tccgacaagg atcagaaaac atatcgcaac ccctgtattc accagccacc
      181 ggggaaaggt tctgcctttg atcttcagtt ccgaaagttt ttgaattcct tttatcatat
      241 aatacatcag ggggagcagc gctgggagca ggtaccgtcc ctgattctga taatccgtgg
      301 tgtatgcata ataaatcgtc agaaatgccg gcataaaaat gcaataaagc atattaatat
      361 ggaagaacca ttctcttccg gatattttgc gacgcttctt atagcagatc acatacagaa
      421 gacttcccgc ggtgccaagc acaaaaaatg ctttatacgc ccgatacagc cagatgcttc
      481 cgtagatgga catggagccg aaagctgcca cgaagctgtg gaacagaccg gacagtgtgt
      541 agcgttcccg gaacatttcc agcaccgtat aacccatgga ctgataagtc tgcatggtca
      601 gcggattgac actctccacc gcatattgga tcgccatctt ttcccgggtg gccagtccca
      661 gcagatctcc gtccagaacg atataactgc ggatgaacca ccagccgatc cccgccagaa
      721 ctactccggc gatcagacag ccgtatttca gcatattctt ccagtcatag aaatagccgc
      781 cgttttcctt cttctgcaaa aaggacaaca gaaagagtaa aatgcagctt acgatatatc
      841 cgtaggcatt ataataagaa agagcacaca gaataatacc accgctcatc cacagactgt
      901 tgcggagatt tataccattc tgatatacgc ataccagagc atataccatc atggccgtgg
      961 ataacataca gcaggaatcc gtattcacat aagtgtgcag gaacaatccc tccggcaggt
     1021 acgttactgc aaaacagaac agccaacgga atcggtcatc ccggaacact ctttttccaa
     1081 tcaaatatac cactactgcc atcagcaggc caaaggttac gttcaccagt cttgcggtat
     1141 agagcagtac cacttccgat tccgtaaaca ggctcacaaa gcgcatgaga tatccctgca
     1201 cgatataggg aaatacgtta tacagagcat aggaaaatcc gtaggccgga atccgcacct
     1261 cttcctccca tcccgtaggg atcttcccat acttgcagat aaactgcggg atcagatatc
     1321 tcgcatgttc atccggcgga ttaccataca gcggcggtgt gttggccagc ggctgcaaca
     1381 gtgcaagtgt aagtgccagt gccaggaatc ccaacagata taatgtaaaa ataatctttt
     1441 ctgatttttt ctgcattctg tcatccttta acatttttat agatacgaag accccaaagt
     1501 ggtaaattca actttggggc ctggtaaact attcagaccc tattcgcaaa accgcacctt
     1561 cggtgctttc cttttgctca tagggttact tcgtaatata aaaataagta tatatcctta
     1621 tgaatgcaag cattcaacgt atatatactt attttttatt actctgaata gtttatatgt
     1681 agaagcttcc ggtgatctct ttgttcatta catagtaaac catgttctct tcgggcttta
     1741 catacagctc tacgctggtc aggtcagctg ccttctgctt cagatcgtat ttccatacgt
     1801 ccttggcgat cttcagcagt tcttcctgag agtaggactt tccaccgaac tgaacgctga
     1861 tctcactctt taactctgtc ttcttggcta cgggcttctt agcctctgcc tttgcaggtg
     1921 ctgccttctt ggtctctttc ttaactgttt ccttcttggc tgcaggcttc ttaacctctg
     1981 cctttacagg tgctttttct tctgcctttg ctacaggggc cttctcagct tccttcacag
     2041 cggtcttggt ttctgtctta actggagttg tcggtgctgc tttcttaact tctgccttag
     2101 gcgcttccac tttagctgct acaggtgcta cagttttctt aactactgcc atgttaccgt
     2161 ctcccttctg tacgaatcat gtacaaaaaa cattttcatt ctcctcacaa gtaccgcatt
     2221 tcggtgctcg ataagggtag tgtattcctt ttccctataa ttgtcaactg ttatgcgaat
     2281 accagaaagt acagcaatac gataataccc agcacaatac gataccatcc aaagacttta
     2341 aagtcatgct ttttgatata tcccatcagg aaacgaagta cgaacaggga tacaatgaag
     2401 gatatcactg taccgaccag caggcaggca agctccattc ccgtgaatgc aaatccgaac
     2461 ttcaatacct tcagaagact cgctccaaac ataacgggaa tcgccagaaa gaaggtaaat
     2521 tccgctgcca ccgttctcga tataccaatc ataatagcac ccaggatcgt agctccggag
     2581 cgggatgttc cgggcagtgc cgcagccacc agttggcaga aaccgatgat caatgcatct
     2641 ctgtaggtca actgtgacag tgttgtggtg gtaggtacac gattcttgtt ccagttctcg
     2701 atcacgataa acaatacacc gaccaatatc agcataactg ctaccacgat gacctggacc
     2761 gttactccac tggttccgat ctctttttta aaggcttcgc tcaccacatc atcaaacagc
     2821 aggccgtaaa cgaccgcagg gatacatgct acaacgatct tcacccacat ccagaaagca
     2881 tccatgctca gtgccacatt tcctaccgta aaatcttttt tagatcttac aataccacct
     2941 ttttttgtct gctgattttt cttcatataa aaaggccaga tcttgttcca gaacagaacg
     3001 actaccgcca ggatagctcc caactgaatg acaacatcaa acatatcgaa gaagccttca
     3061 ctggtctcct gaaaaggaat cagacgctcc accaggatca gatgtcctgt gctgctgatg
     3121 ggcagccact cggtgatgcc ctccacgata ccgtagataa ttgccttgat aatttctaac
     3181 attttgtttc tcctatcttt tttattttct gacgaatcag agcttttatt tttcgagata
     3241 tgccagcaga tcttcatagc agttctctgc ttcctgatag ccctcttcat ataatgcgat
     3301 cagtttgtcc ttgtctttct ctatccgtcc cacatcactc ttcttatgag gtctgatcac
     3361 gaaagcctga ccgtttttct cctgtctgtc aatatagtcc aggcactcgt tataacggat
     3421 atgtcggtct gccatgagtt catatacttt aggatatttc aggtatctga gttttatgag
     3481 tcccagctgt gacgcagatg ctggctttct cacgaatccc acctctttgg tcatgaccaa
     3541 gatgttcttc cggttgccgt ccagaataga tttctccaga ggaatggcat cgctgatgcc
     3601 accatccaga tacagcttcc catcgatctt cacattccgt gccaccagcg gcagggatgc
     3661 ggatgcccgg atcttgtcga tatccttgcg gatatcacgg acacgcagat actccggctg
     3721 tcccgtctcg atgttggtca ccacgctgta ggccttgcca ggatattttt caaaggtatc
     3781 gtaatcatac ggatacaggt aatccggaat caggtgataa cagaaatcca cattgaacaa
     3841 atctccgctg gtcaataagc tttccaggct gcaataccgt ctcgtatcca gataatccac
     3901 actgacactc agcgcccgtc ccttttgtct ggacaggtag ctgcacatat ggcatgcacc
     3961 ggcggatact ccgtagatgt gggaaaaatc aattcccttt tccgtaaaaa agtccagtac
     4021 gccggcggtg tatacaccct tcattccgcc gccttccaat accagtcctg cctgatacat
     4081 tctcattact ctcctttctg cttctgcaac tatccagaac cccattcgcg aaatcgcatc
     4141 tgcgatgctt tcctgaataa caagttctct tgttatttgc tcatgtggtt nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcacccggta ccagaacgag atcatatttc
     4321 aatgctttct ggctgaatac cttggcatcc tcttccagcg ccttcgggaa aatgtagaag
     4381 gttccgccgg gcttcacaca ggtgaatccc aagtctagca gttctttata gagaatgttc
     4441 atattcgtct catacaccga cagatccgct gtcttatcca gtacctgtgc cactgccagc
     4501 tggataatgg aaggaggaca gttatgtccg ataccgcggg agatctgtcc gcacatattg
     4561 atcatggtct ccgcatcctc acatgccggg ttcactgcca cataaccgat acgttctccc
     4621 ggcagagaca gggatttgga gaaagaatag cacatgatgg tattgtcata aaaataggat
     4681 acgcagggac tgtctacacc ttcaaacacg atctcacgat aaggttcatc agagatcaga
     4741 taaataatat aaccgtattc ccgtgacttt tctctcagga tatctgccag tctctgcaat
     4801 gtcggggtgg aatataccac accggagggg ttgttgggag tattaatcag tactgccttg
     4861 accttctccg tcagcatctc ctcaaatgcc tcgaagttga tctggaagtt ctccagattg
     4921 ggcggaacca ccttcagtac cgctccggtg agatccacat aaggatgata ttccgggaaa
     4981 aacggtgcaa aagtcaggat ctcatctccc ggctccgtca ccagacgaaa agcatgtgcc
     5041 agcgctcctg ccgccccgga tgccatgaaa atgtgctcct tacgataagg cagcccaaac
     5101 ctcttctcca ggttctccgc aattgcctcc ctgacgcttg taataccgag gctgggactg
     5161 tagccgtgca gttccaacgg ttccttcgtc gccagcatat ggatcatgcg gtcagtaaat
     5221 tctctgggaa ccggaacgga aggattcccc aggctgtaat caaatacgtt ctcgtatccg
     5281 atttcctgtc cccttgccgt agcaaattcc gaaagctgcc gaatcactga ttttccggac
     5341 agcatatctt tatatttctg aactaacata ctaatctcct ttccgcagac gattcctttc
     5401 gtcggaggaa tcgcaaatca aatttcaaat ttgattctgc gctgtttatt tgatctctat
     5461 aaacttatct ctgaaccttt ccgtggacag acagatactg tttcttccac agcttttggt
     5521 cttatagaga agatcatccg ctcttgatgt gaaatcccat tccttgctat tgcccaccgg
     5581 aatccgcaca aatactccct ggcttaccga tacatagttg ctgcaattac tgcattcatg
     5641 gggaagtttc aatttgcgga cctccctcaa aatatcttcc gccactcttc gtattttctc
     5701 caccgtcatt cctgtataga ggatcataaa ttcatcccca ccgtatctgg cacagaaaat
     5761 cttacccgga acctgctgtt tccgcagaac tccggcaatc gctttcaggc actgatctcc
     5821 ggccagatgt ccgtaatgat cgttgtattc cttgaagaaa tcaatatcca tgagttctac
     5881 tcccatgagc tcatgttcct cataggcatg ttcaaaactg ctcgtaaggt attcatgtaa
     5941 aaaggtccgg tttgccagtc ccgtcagctc atcatgcata gccagacttt ccagctcccg
     6001 gttggaagct ttcatgttcg tccagtccaa cgtaacgttt ttcagccggt tctgcaattc
     6061 tatgacattg atcagcgctt ctctgttttc ctgttggtat ttctcataaa tatcaaaata
     6121 ctggcgggta taatctatgt attcccaggt aagacccttc ttctgcagaa gttcactctt
     6181 gaaaggatac agatccagga aaatattata atgctcttcg atatgctttt catcaatgat
     6241 ccggatcaat tcttccagat attttccatc aggcaggatt gccaggattc ccgccaggat
     6301 caccagttca tccatgatct cttcaaaaac atcccggtct gacaattgct ccagaagatc
     6361 tgctgcaagc tttaaagcct cttcctcatg cccttgtaat aattcgcagg aaatacgata
     6421 catttctaat gttattttcg aataatagct ttccggagcc tctctgatga tctgttctat
     6481 ttgattccaa agccgtaaag cctcttccat ttctcccata tacatgtgac agcagccaca
     6541 ttcgatcata cagttgatcc tgttataatt cagctgatat gttttctctg acttactata
     6601 gaagtcgatt gctgtacggt aatgctgtat cgcctcctgc cgcaggcgca tcctgaccag
     6661 ggtataggca atattactct ccgccatgcc ctggatataa taaaaatggt atttttccgc
     6721 atattgaagg cagttataat aggaacttaa ggccaccatc cggttgttct tccgatcgga
     6781 caccgctccc atcatattat aggttctggc cagcagttca tacatctgct catttttcag
     6841 ataaccgata ctctctgtca gacattgcaa ggtgttttca tactggtcgt tctgccaata
     6901 atattctgca agataataaa gccccagacc ggccagcttc tgtgagccgg tgtctgctgc
     6961 atattccatc actttctgac aggtctcgtt gtccggaaat ttaccgtttt cccggttttg
     7021 gatggtttcc tctatgaaat tttgaacggt ctgtggtaaa ccctttaaat ccatgccgat
     7081 tcctttccgg aatccccaag ctgtcattga tcatcccctg tcagatgcca tcataaacag
     7141 ccatggtatt ctaaatagtt atcataccac agtactttgt cactttacaa gattgctccg
     7201 ccaaaaatac acgtcacccg taaaaaatta aggttttatt tagatttcat cctctgccca
     7261 tgcaagagca catttcaccg cacgatgcca gccctttaac agtttcttgc agtcttcttc
     7321 atccatttcc ggtgcaaagg atgctccgat ctgccagttc tcacggatct catcacgatt
     7381 cttccaatag cctaccgcaa gtcctgccag ataagccgct cccagagcag tcgtctcgat
     7441 acatgccgga cggcgtacct gggtgttgac gatatccgcc tggaactgca taaggaaatc
     7501 attggcactg gcaccaccgt ctactttcag gctctttaaa tcaatgccgc tgtccttctc
     7561 catggcttcc agcacatctg ccgtctggta tgcgatggat tccagcgtgg ctcggatgaa
     7621 atgttccttg gtacagcctc tggtaatgcc gacgatggtt cctctggcat actgatccca
     7681 gtagggggct cccatgcccg caaatgccgg aacaatatag acccctgcgg tatccggcac
     7741 atgctctgca tactcctggg actgtgcaga ggtcttgagc atacgcatac tgtcacgaag
     7801 ccactggatc ccggcaccgg ctacgaacac gctgccctcc agggcatatt ctacgttctt
     7861 gcttgtgctg gcagcaatag tggtcaatag acccgactgg gaggtaatgg cttcgtgtcc
     7921 ggtgttcatt aataagaagc agccggttcc gtaagtgttt ttcacttctc cctgctcaaa
     7981 gcagcactgt ccgaacagcg ccgcctgctg gtctcccgcc gcaccggcaa taggaatctc
     8041 gccgcccaga acggctgcat cggtcctgcc gtagatctca ctggaacagc acaccttcgg
     8101 cagcagactc tccgggatat tgaagcgctt caggatctcc tgatcccagc agagattatg
     8161 aatatcgaaa agcatggtcc gggaagcatt cgtataatcc gtcacatgta cggcaccctt
     8221 agtcagattc cagatcagcc aggtatccac cgtaccgaaa agcaattccc cccgctctgc
     8281 ccgttctctg acaccgggta cattattcag gatccaggca atcttggaag cactgaaata
     8341 agcatccggg atcaagccgg tcttcttccg gatgatctga tcaaagccgt cttttttcag
     8401 ttcctcaatc atatcggaag tacggcggca ctgccataca atagcattgt atacaggttc
     8461 gccggtattt ttatcccaga taatcgtggt ttcccgctgg ttggtgatgc cgatggcctc
     8521 gatctggtca tcggtgatac ccagcaccgc catactttca atggccactc ccagctggga
     8581 ggaccagatt tccatagggt tatgctctac ccagcctggc tggggataga tctgtgtgaa
     8641 ttctttttgt gccatggaac agatatttcc ctgtttatca aacaggatac agcgggaact
     8701 cgtggttccc tgatccagtg ccatgatgta tgtctttttc tgtgtgttat gtgtatctcc
     8761 cattgtgtta tgtaacctcc cgttgttcgt tccaggctct gcccatagca tatcccaatc
     8821 gtgtcattgg aatatattgt gtccattgcc ccaaatatgc tccattctca atagttctgt
     8881 cctctgtcac ccacgaaacg accgggttgc cactatattg acacccgtgt ctgctacgtt
     8941 ttccacaatg acatctccta catgaatggg tgcagccgca gtgacatctg ccagggcttt
     9001 catgcagtca tcgatcctgc ctttgggaat atcggaagcc gtcttcacgg atactactgt
     9061 gtctggatac ccttccacac gcacggtgga ggtgacaatc cgggtaggat tcgtgacttc
     9121 tttttctccg taagccgccc ctctggggca ccggttgccg gtcactgtga cactattgtc
     9181 accttctatg gtcacagtca gagcacaccc caccgggcag cagatacagg tcagttctct
     9241 ttgtgttgtg ttcttctctg ccatcgtctc accctctttt ctgccgaata tattctacag
     9301 cgtagtttcc cgccttttcc gcttctgtgg atacatggtc taccagttcg tgtacatgga
     9361 gcacattgcc gcaggcgaag acgccgggga tactgcactc cagattctct cccactaccg
     9421 gtcctccggt cttgccgtcc atatccgctc ctaagcccct ggtcagttcg ttctccggga
     9481 tcagcccgca ggataacaac agagtatcgc agggaacctc ccactcggat ccggggatgg
     9541 gttttccatt atcgtccact tttgtcaggg tcacaccctc tacacggtct ttgccgtgga
     9601 tctttgctac cgtagtattc agatacagtg gaatattaaa atcatccagg cactgtacga
     9661 tattacgctt caggccgcca ctctgggggc gaagctctgc cacgcactgt actttggcac
     9721 cttccagcac catacgtctc gccatgataa gtccgatgtc tccggaacca aggatcacca
     9781 catatttacc gggcatccgc cctctgatat ttacataata ctgtgccgtt ccggcgctgt
     9841 agatgcctgc cggtctgctg ccggggatat tcagtgctcc ccgggaacgt tctctgcatc
     9901 ccatagccag gatcacggtc tccgtctcga tttccaacag gccgtcctct ttgttcatgg
     9961 cagtgacttt cttgcgtcca tcggagcgca gggaaatatt tgttaccatg gtattca
//
LOCUS       DAWEJW010000101         9718 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2390_length_9718_cov_72.428128, whole genome shotgun sequence.
ACCESSION   DAWEJW010000101 DAWEJW010000000
VERSION     DAWEJW010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9718)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9718
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_9718_cov_72.428128"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2969..3068
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5421..5520
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2490 a         2046 c         2609 g         2373 t
ORIGIN      
        1 tgggatatgt ggtgtatctg ctgggatcga aaaagggaaa gcggattttg ctgatagtac
       61 ccacactgct ggtgtttgcg gtatgcattc tttccccggt caacggagaa ctgcgctata
      121 tgctgccggt gatgataatg cttccgctaa atctgacatg gtgtgcttat caggaaagag
      181 aatctgttgc agaaaaagag gagaaataaa atgacatata aaaaaccgga acttttaatc
      241 ccggccagca gcctggaagt attaaaaacg gctgttattt ttggggcgga tgctgtttat
      301 atcggcggag aagcttttgg actgcgggcc aaggcaaaaa atttcagcag tgaagaaatg
      361 gcggaaggag ttgcattcgc ccatgcacat ggagtgaagg tttatgtgac ggccaatatt
      421 ctggcgcata attatgatct cgagggagcc agaaaatatt ttgccgagct gcgggatatg
      481 aagccggagc agccggatgc gctgatcatt tccgatccgg gaatgttcat gatcgccaaa
      541 gaagtctggc cccaggtgga agtgcatatt tccacgcagg ccaacaatac caactatgag
      601 acatatctgt tctggtggaa gctgggagcc aaaagagtgg tgtctgccag agaactgtca
      661 ctggtagaga tcaagggaat ccgggagaag attcctgcgg aaatggagat tgagagtttt
      721 gtccacgggg caatgtgtat ctcttattcc ggcagatgtc tgctgagcaa tttctttaca
      781 ggaagagatg ccaacagagg tgcctgcaca catccctgcc gctggaaata tgcggtggtg
      841 gaggagaccc gtcccggaga atatctgccg gtctacgaga atgagcgggg gacatatatt
      901 ttcaactcca aggatctgtg catgatcgaa catattccgg agctggtgga ggcaggaatc
      961 gacagtctca agatcgaggg gcgtatgaag accgcattgt atgtggcaac ggtagccaga
     1021 acctaccgga aggccattga tgattatttt gccgatccgg agctgtacga gaaaaatatg
     1081 gactggtaca aggcagagat ctccaagtgc acctatcgtc agtttacgac ggggttctat
     1141 ttcggcaagc cggatgaaaa tacgcagatc tatgataata acacttacgt gaacgaatat
     1201 atttatctgg gaattgtgga ggcagtaaag gattctctgt gccggatcga gcagcggaac
     1261 aaattctgcg tgggcgattc catagaaatc atgaagcctg acggcaccaa tgtaccggtc
     1321 acggtggaag ctatgtacac cgaggaggga gaaagtgtgg acagtgcacc ccatcccaag
     1381 caggtgctgt ggataaagtt atccatgccg ccgcaggaat ttgatttgct gcgtcagaaa
     1441 aatgctgatt gagaaagagt gaccggaggt aacgccttat gaaaagggaa ggcagaattc
     1501 ataaaggagt gtccataggt gcctatagta ttctcatcgc ttttatgctg ttgctgttgt
     1561 ggacagtact caggtatccg gggaatcatc tgatcccatg gcagatggct ctgggggtgg
     1621 cggctgctac agtgttgctg ctggcggccg gtgcggcatg gaataaaata tatacggtga
     1681 caggccggag aaattcgctg tatgccatag cgctggtatt gtttggggtg ctgctttatg
     1741 tggtttcatt gagccggcag gggaatgaga atactctgtt ggattacacc tatgtctatc
     1801 gggatgccct gaatctggcg aacgggagag aactggaaga caccaattat tttttgacct
     1861 attcgaataa tctgaagccg atgcttttgc tttccgttct gtttcggatg gcattgctga
     1921 tggatgtgtc cccgttttat tttgttttgc tgcggaatgt gattctggtg atgctggtgg
     1981 catgtgcctg tggttatctt gcggaacgga atggggatac ttgctggaga tttccgatcc
     2041 tgcttgcctt tgtatttctg cttccgatgt gggagatgac tgccgtgttt tatacggatt
     2101 ccatgagctt cggtatggga atcctgggac tggctttcct gaaattggcg gcagcatcca
     2161 gggggaagcg ccggcagatc ctgtgggcgc tgtttgcggc cggtatggca gtactggcag
     2221 gcacatggaa gatcacagtg atcataccgc tgatcgcatg cgctgtgatc ctcttgtggc
     2281 agagagtgtc ggtggaccgt cgagtgacat tgctgtttgg aggttttgtc gtgattctgg
     2341 gagtggctct gcagctctgg gccaattcct acgagatcac aaaggaagca tctgaaacag
     2401 ccaatcctgt gatcagctgg gtggcactgg gaatgaagga agacggttcc tggaccaaca
     2461 acacagagtt tgtggatcat atgtatgagt tttccaccag acaggaaaaa cagcaataca
     2521 gtatggaata tattcgtgaa aacagatcgg aattctggaa tcccgtacat ttgcgcaaaa
     2581 aggcaagcta taattttgcc aacggaaatc tgggggccag tgcattttta aacgtggagt
     2641 acagcgatgg aaccttgctc tgggaaatgt tttcgccctg gggaaaatat tactggcgca
     2701 cctgtcagta ttgtttctgc tatctggtgt ccatgtatgt gctgttactg atcggaatga
     2761 tcctcagcct tcgcaatatt atcagagatc aggaaccacc tgtaatgttg atgatctgtc
     2821 agttgggatt tatcggtatc gttgtttttt taatgttctg ggaagccagt ggaagacaga
     2881 tgtacaatca gatgcccgga atcctgctgg gaagtgttct gagtatagag tatttttgga
     2941 aaaacttatc actaaaacct cgaaaatann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnngt ataccgaagg gaagtaggga catgcgcgaa gaattgaatg tagaggagct
     3121 ttttgccagc aaggtgttta ccctgggcaa gatgaaagag aggctcccaa agagtactta
     3181 caaagaagtg aagaagatca tggatcaggg aggagaactt tccctggcaa ctgcggatgt
     3241 agtggcaagt gccatgaagg attgggccat cgagaacggt gccactcatt atacccactg
     3301 gttccagccg ttgaccggca ttaccgcaga gaaacatgat gcttttgtga cacatcctga
     3361 tgaggatggc cggatgatca tggaattttc cggcaaagaa ctgatcaagg gagagcccga
     3421 tgcatcttct ttcccttccg gaggcttacg tgctaccttt gaggcaagag gttacactac
     3481 atgggacatc acctcccccg catttatcaa agagaccgct accggtccga ccctttgtat
     3541 ccctacagca ttctgttctt ataaaggcga agcactggat aagaagacac cgttacttcg
     3601 ttccatggag gctctgagca agcaggcgat ccgtatcgtc cgcctgttcg gcaatacaga
     3661 agctaccaaa gtactgcctt ccgttggcgc ggagcaggaa tatttcctgg tagattctgc
     3721 caaggctttg cagcgtgaag atattatttt cgccggacga accctgttcg gtgctcccgc
     3781 ccccaaggga caggagatgg atgaccacta tttcggtgtt atccgtgagc gtatcggtgg
     3841 tttcatgcat gatgtcaata tcgagctgtg gaagctgggt gtaacatcca agacccagca
     3901 taatgaggcg gctcccgcac agcatgaagt ggcgcccatc tacgagtctg ccaatgtggc
     3961 agtggatcac aaccagctgg taatggagac tctgaagcgt gtggcaggca agcacggtct
     4021 gcgctgcatg ctgcatgaga agccttttgc cggagtaaac ggttccggta agcataacaa
     4081 ctggtccatc actacggaca atggtgtaaa ccttctggag cccggccaga cccccaatga
     4141 aaatattcag ttcctgctgg tactggcatg tatcatgaaa gctgtagata cccatgcgga
     4201 tctgctgcgt cagagtgcgt ccaatgtagg taacgatcac agactgggcg gttacgaggc
     4261 accccctgcc attatttcga tcttcctggg cgaacagctg gaggatgtgg tggcacagct
     4321 ggtggagacc ggtaaagcag acaatctgaa agaaggcggt gtacttcgca ccggtgtatc
     4381 cacattgccg gattttgtaa aggatgctac cgacagaaac cgtacttcac cgtttgcctt
     4441 tacaggaaat aaattcgaat tccgtatggt gggaagcgag gattccatcg gaagccccaa
     4501 caccaccctg aatgccattg tggcagaggc gttctgcgaa gctgcagacc gtctggaagg
     4561 tgcggaagat ttcgatatgg cagtccatga tctgatcaag gaatatatga cggagcacca
     4621 gcgtattatt ttcaatggca acggttatgc cagggaatgg gaagaggaag cagccagaag
     4681 aggactgccc aatattcctt ccatggtagc agcagtagat actctgacta ctcccaaggc
     4741 tatccatctg tttgaaaagt tcggtatttt caccgaggca gagctgcgtt cccgcgcgga
     4801 ggtactgtac gagacctatg caaagaccat caatatcgag gcactgacta tggtggatat
     4861 ggcaaacaag cagatcattc ctgcagtaat gaaatacagc aagtcgctgg cagatgctgt
     4921 tattgctatt acggaagctg gcggagacac ctacgtgcct acccgtatgt tgcagcagat
     4981 cacacagaag ctgacagaga tggaaaaggc atccgaggca cttctcgagg tggagaaaaa
     5041 ggcttccgcc atggagagag gcaaggagca ggcattctgt tatcatgatg aagtcatggt
     5101 ggcaatgaac gcactgagaa aacctgcgga tgaactggaa aagctggtag acaagaagta
     5161 ctggccgttc ccgacctatg cggatctgtt gtttgaggta tagagcactt cagagatcgc
     5221 ttcggatgca aaagtaaagc ctcgtagagg cgattttgcg aatggagttc tgaatagtta
     5281 cgattttttc aaaaaaggta ttgcattcaa aaaaaatctg ttgtatagta atgaaccata
     5341 gaggagcgat cctcacaagt taatattttt caatgcgaaa cgaaggcgtt tacactagga
     5401 agtgtagcgc ctttttttgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 agtcgcagga agcatggaaa atagcaagaa taaggaggaa acgaagatga gtgaagtatt
     5581 aaacgtggaa gaaatctttg gcagtaaggt attcactctg ggtaagatga gggaaagatt
     5641 acccaagagc gtatacaaag aagtgaagaa gatcattgat cacggcggag agctgtctct
     5701 ggccaccgct gatgttgtag caaaggcaat gaaggattgg gccatcgaga acggtgccac
     5761 ccattatacc cactggttcc agcctctgac cggtatcacc gcagagaagc acgatgcatt
     5821 cgtaacccat cccgatgagt acggcaagat gatcatggag ttctccggta aggagctgat
     5881 caagggcgag cccgatgcat cttccttccc ctccggaggc ttacgagcta ccttcgaggc
     5941 aagaggctat actgcatggg atatcacttc tcccgcattc ctgaaagagg atgcaaccgg
     6001 cgtgatcctc tgcattccca ctgcattctg ttcttacaag ggtgaggcac tggataagaa
     6061 gacaccgctt cttcgttcca tggaagcagt cagcacacag gcactgcgta tcgtaagact
     6121 gttcggcaat accgaagcta ccaaggtcgt agctagcgtg ggaccggagc aggaatattt
     6181 cctggtagac agagacaaat acttaaagcg tgaagacctg atcttcgcag gacgtaccct
     6241 gttcggtgca cccgcaccca agggacagga gctggaggat cattacttcg gtactatccg
     6301 cgagagaatc ggttctttca tgaaggatct gaacattgaa ctgtggaagc tgggcgtaac
     6361 cgccaagaca cagcataacg aagtggctcc cgcacagcac gagctggctc ctatttatga
     6421 gaccgccaac attgcggtag atcataacca gctggttatg gagaccatga agaaagtagc
     6481 aggacgtcac ggtatgacct gcctgctgca cgagaagcct ttcgccggag tgaacggttc
     6541 cggtaagcat aacaactggt ccctgggaac cgataacggt gtcaacctgt tggatcccgg
     6601 cgatactcct aacgagaaca tccagttcct gttagtactg gcatgtatcc tgaaagccgt
     6661 agatacccat gcagatctgc ttcgtcagag tgcatccgat gtcggcaacg atcacagact
     6721 gggagctaac gaggcacccc ctgccatcat ttccgtattc ctgggcgagc agctggagga
     6781 tgtggtaaag cagctggttg agaccggtga tgctactcat tccatccagg gcggtaaact
     6841 gttgaccggc gtatccactc tgccggatct ggataaggat gccaccgacc gtaacagaac
     6901 ttcaccgttt gctttcaccg gcaacaagtt tgagttccgt atggtaggta gtgcagactc
     6961 catcgccagc cccaacacca ctctgaacgc tatcgttgca gaagctttct gcgaggcagc
     7021 agacattctg gagaaagcag acgatttcga tattgccgta cacgatctga tcaagaagta
     7081 cctgaccgag catcagagaa tcatcttcaa cggcaacggc tattctgagg agtgggtaga
     7141 agaggcagcc agaagaggac ttcccaacat caagtccatg gtagaggctt ccgagaccct
     7201 gaccaccgag aagtctatca aactgttcga gaagttcggt atcttcacca aagctgagct
     7261 ggaatcccgc gaggaggttc tgtatgagac ctactccaag accatcaaca tcgaagctct
     7321 gaccatggta gatatggcga agaagcagta cattcccgca gttatggggt ataccaggac
     7381 tctggcggat accgtactgg ctgttaagag tgcaggtgcg gatgcaaccg tacagaccac
     7441 tgtactgaag agcatttccg agaagctggc tgaggcacag gctgcagaga ctgctctgga
     7501 ggataagctg gctgaggcag caggcatcga agatcccaag aagcttgctt tcttctataa
     7561 agatgtggta cataccgcaa tggatgacct gagaactccc gtagatgaac tggagatgat
     7621 ggttgacaag aaggtatggc cagtacctgc ctatggtgat ctgatctttg aagtataaac
     7681 agcattgtaa aataccatca tacgtttctc tcattgtgtt gaataaaaac ggggcttgcc
     7741 gcaggacggt gggcctcgtt ttgcagttat gtatgtttct tgtcattgct atggggaaaa
     7801 tcctgtgtta tgataactgc atgatgaaaa atatactgaa aaataagaaa ttttggaaaa
     7861 ttatctgtat tcttgcaatc attgcatata cggcaaaaaa tttatttatt ggtgcagata
     7921 cggacgaagg ctatggcatc atggtgggct atcgtcttgc catgggagac cggctgctgc
     7981 ttgaaatgtg ggagccacac cagacttccg ccatttttac ggcagttttt atcaggctgt
     8041 ttgtgatgct gaccggtgga gtgaattatc tgaatctatt tttacggctc gtatttttcc
     8101 caatccaggc gtgcgtcagc gtctttctat ataagacgat ccgcaggacg gtgccgcaga
     8161 tggatgagaa tgtggcagca ctgatggggc ttctctacta tgtgacaaca cccaagtcca
     8221 tttttattcc ggagtattcc aatcttcata actggttctt tgcacttatg gtgctgtgcc
     8281 ttctgagata cttcggagcg aaggactccg agggcagaca gacagcagga gagctgcggt
     8341 ggctggtgct ggcaggtata ttcatgacct gtgatgtgct ggcttatccc agtatggtac
     8401 tggtattttt gtgctgcctg gtatttctgc tggtgcgcag atcagagaaa aaatggaaag
     8461 aactctgtgc ttatgtactg ccctgtgtgg caagtgcggc agtaatgttt acatatcttc
     8521 tgagttatat gacaccgcag aaaatgttgg agatggcagg cgagatcctg ggagaggggt
     8581 cccatcagac tactgtggga gaaaaattgt tgggatgggg gtcaagcctg ggcgagatgg
     8641 ccatgatcct tctgtgtgcc ctgctgctgt ctctggggat ccgatggttt atagagaagg
     8701 tatggaagaa aaaacttccc ggactgctac agcttccggg catgctgttt ttcgtaattg
     8761 tatttctgat ccagatctgg ttctggctgt tcagttcctt taatgcaatt tacccgcagc
     8821 ttttgctgat ggcagtatcc ctgacgggat gttataccta tctgcgcaga gacaaaacgg
     8881 aaaggctgtt ctatgaactg atcctactgg catttgtcaa ttatttttcg gtacttctgt
     8941 tgtctaactg gggaccgatg ctgctggtca cctatctcat tatgggagca gtggccggac
     9001 tggtatgtct gggagactac tggcagaagg aaaacaccgc aggaaagaag gcaattcaga
     9061 tcctgtgcct gatccttgtg cttgggaatg cattttccta tacatggctg attctgggat
     9121 cccaggaata tcatgcctat gtgattcaaa atgtgcgtgg catcaacaga gagggattac
     9181 gcgccggaat cctcacggat tatatgactg cataccgtta caacaataat ctggctgtat
     9241 ggccggaggc cgtgccggac ggcagtaccg tgctttatgt gggaccgtcc cagttctatt
     9301 gtatgctcgg agaacataag caggcatccc ccgatacgat ctgctccatg atctatgatg
     9361 agcgtctgtt ggactactgg aagatcaatc cggacagata tccggatgtg gtggtatttg
     9421 aaagctgtta tggagatatt gcccaggaag gagagaacag ctttatctgg aactggttga
     9481 tggaggattt taagcctgca gagaggaagg attaccccta tatcacagtg tttatgcgat
     9541 gattttacgg aaattgttct aatttcatat attttttaaa atttttttaa attgggtatt
     9601 gcaaaatggg aacagatagt gtatacttca tcaagtaaac gacatagggc tatcgccaaa
     9661 cggtaaggca acggactctg actccgtcat ttcaaggttc gaatccttgt agccctgc
//
LOCUS       DAWEJW010000102         9640 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2410_length_9640_cov_61.952634, whole genome shotgun sequence.
ACCESSION   DAWEJW010000102 DAWEJW010000000
VERSION     DAWEJW010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2410_length_9640_cov_61.952634"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3400..3499
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2634 a         2079 c         2561 g         2266 t
ORIGIN      
        1 atcctctacc tggcagcgat caccggtatc aacggagaat tgtacgaagc agccatgatc
       61 gatggagcga accgttggaa gagaatgtgg catatcacac ttcccggtat caagagcaca
      121 gtagtaacac tgctgatcct gaacctcgga cgtgtcatgg gaagtaactt agagcgtctg
      181 accgcattag aaaattcaca ggtcaaggac ttccagtatc agctggctgt ctacatttac
      241 aacaaaggta ttggagccgg aaaattcagt gcggcgaccg cagttggtct gttccagtca
      301 ttgatcggat tgatcctggt tctgttatct gataaattcg caaagaagct tggcgaagac
      361 ggattactgt aggaggtgag agagatgaag aaaaaagaag ttaatattaa tgaagaatct
      421 acctccgcag gcggagcaca tgcggtcaac aaattccgca tcagtgattt cgtcatgatg
      481 gcagtgatcg tcatcctctg cgccagctgt gtattaccgt tcttacatgt agcagtaaaa
      541 tccatatccg gtaataccgc agtaatgtcc aagagcgttt acttctggcc caaagacatc
      601 accttcgatg cttacaagaa ggtatttgca gataactcca tgacctactc catgatcttc
      661 tcggtgatcg ttaccgtgat cttcaccgct ctgggaatgc tggtatgtac ttgtgccgct
      721 tatcctcttt ccaagaagag actgaagggc aaaaagttac tgactttcct gctgatggta
      781 ccgatgtact tcagtgccgg catcattccc agctacctgt tatatcagaa gctgcatgtg
      841 ctggatacca tgtgggtgtt gatcttacct ctgatctatt ccccgtacaa catgctgatc
      901 atgaagaact acttccagag tacgattccc gacagcttag aggaatccgc tttcctggat
      961 ggcgcaagta acttccagat cctgagcaag atcgtattac cgctgtccaa accgatcctg
     1021 gcaacgttgt ctctgttcta cgccgtaggc cgttggaact cttacgcaga caacatgtac
     1081 tacaccaaga gtgctaacct gaagctgatc cagtacaagt tatatcagtt ggtagcatcc
     1141 gcagcagaag cacagacttc ctccctggca gataccggca gtgcgatcca gtccactccc
     1201 gaagtattac aggcagcaag tatcatgttt gtaacaattc ctatcattat tatttatcct
     1261 ttcctgcaga agtacttcgt acagggtacc atgatcggag cagttaaggg ctaaaagtta
     1321 aaaggaacaa aacaaccaag ttagttttcc ccaaaaccgg gggtacacat aaaactatgt
     1381 ataattttta aggaggaaaa tcctctcctc gccagggact cggatttttc cttcggaaaa
     1441 acaaggagga ttgatttatg aagaagaata ctcttcacaa ggtaatggca acagcacttg
     1501 ttggtgctat ggcaatgagc actctgaccg gatgcggcag cagtgcggac aacacaaccg
     1561 cagatgccgg aacaaaggaa tctgtagcag agagttctgt agcaagcaca gatgcaggag
     1621 catccacagc agtagcaggt attgacggat gggaggcttt cgcagacaat gttactctgc
     1681 agatccccgt atacgatcgt ggttcttccg gaaacggatg ctctgatgta gagaacaact
     1741 actggaccaa ctgggtacag gagaacttcg gtgataagta caacatcacg gtagaatatg
     1801 taggaattac ccgtagcgac gttatgactg attacgctat gctggcagca agccagagcc
     1861 ttcctaccat ctgtatggaa tatgactatg acaagctggc aagctggcag gcagacggct
     1921 atctgcagac ctacgatgta gaagagttca agcagatcgc tcctacttac tgggagacca
     1981 tggtagagca cggcaacgat gcttacacca agctggatga tgaagattat ctggtactcg
     2041 gttacagacc ttacggtaac accacttata cctggtgcac tttctatcgt caggactggc
     2101 tggaagaagc tggcttctct gagtatcccg ttggcgacaa caccaagctg ttagagctgt
     2161 atgcaaaact ggttgagaac ggccatcagt atcctttaag cggtaagaaa gtaagcggcg
     2221 caggtattga ccagaactat ggttacagag attatcctca ggatgagacc acatgggcaa
     2281 ccaccggtga ctaccagatc cccgctctgt ccaccgaggc tcagaagaga ttcttaaaat
     2341 gggagaacga gctgtacaac gatggttact acaatcctga gtactatatg agagatgctt
     2401 ccgaggcaga ggcagacttc atcaacggtg aagcatttac ctggaccgga tatatctcct
     2461 ccagcatgaa cgtactgaac tccttctatg atgctaaccc cgatgccaag ctggcagtag
     2521 cagtgactcc ttccacctgg acacaggatg agacctgggg atcttccgct tcttacagac
     2581 ccggaaccaa cttcggtatg atgatcggct tcgctaacga tgctaccgag gatgaagtaa
     2641 aagcagctat gatgtatctt gagtggctga accagcccga gaatctgttc accatgcagt
     2701 ggggtattga aggcgtgaac ttcaactacg atgacaacgg cgatcccgta gcagtagcag
     2761 atcagactgg cctggatgag cagcagggac acaacaacaa cgtagactac tggtgtgcag
     2821 taaccgcagt taagaccctc ggatctatcg agaaagatat ccaggcagtt acccctcagg
     2881 gtattcctca ggacttctat gatcagatcc tggctaacta caacggacag gttgctatct
     2941 atgaagcagg caaggctaac tccgactgtc tgttcgcaac cgctatcgat gctgttaccg
     3001 agtacaatca gacgttgtat gatctgtata ctgagtaccg tgacaagctg accatgtgcc
     3061 ctaaggacca gttcgattct ctgtatgaag aactgagcca gaagtacctg gatgcaggtt
     3121 atcaggaagt tatcgatgag agacaggctg ctctggatgc aggtaacacc accagacttc
     3181 agtaagtaaa aagttttatt acaggaagta catacagccc ccaggcatga atggcttggg
     3241 ggctatatta aaaatgcaac tattcagagc catgtaaata ttattcaatg ggcgtcgaat
     3301 gcttgcattc gtaagaacat ttgataatat ttatagggcg aagttacttt atgtcctttt
     3361 ccgaccgact gttttacaat aaaaacaact gtgggaaaan nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnt actttatgtc cttttccgac cgactgtttt acaataaaaa
     3541 caactgtggg aaaagaatat aaagagccaa aaggcagaaa tggaggagct atgttaaaac
     3601 tggaatatga taagaaagaa atagagggag tcattgacaa gatcgtcaaa aagactatga
     3661 atatggatct cacctgggac tggccctgtg gtgtggcata ttacggaatc agcgatgcct
     3721 atgagaagac aggcaagaag gaatatctgg atctgttaaa ggatcgtatc gacgaactga
     3781 tcgatctggg actgcccaag gtatggacag taaacgcctg tgccatggga cattgcctga
     3841 tcaccttata ccaggccacc ggagagcagc agtatcatga cattcttatg agcaagatcg
     3901 cttatttaag aaaagatgcc ctgcgtttcg gagatcatgt attgcagcac accgtatctg
     3961 ccaacaacga tttcccggag cagtgctggt gtgacacttt gttcatggca gcgttcctga
     4021 tgctgcgtgt gggcgtgatg gaacacgatg aagaactgat cgatgatgcc ttgaaccagt
     4081 actactggca tatcaaatat ttacagaatc ccagcagtgg actctggtat cacggctatg
     4141 acaatattgc cggagatcat atgtccggca tctattgggg aagagccaat gcctgggcag
     4201 catttaccat gtcgcaggta ggcggtattc tgcctcagtg ctatctgtat ccgaagtata
     4261 tcgatgtggc cggctcctta aatgagcagt tggcagccct gaaggtatac cagacggaga
     4321 atggattgtg gagaaccgta gtggatgatc cggaatctta tgaagagatt tccgcatctg
     4381 caggaattgc cgcagcaatg ctgaccagag gcaatccttt acacagcaaa tatatcaaca
     4441 aggccatcaa gggcctgctt gccaatgtca gcgaggatgg taaggtgatg aatgtctccg
     4501 gaggaactgc cgtcatgaga gacaaggaag gatacagagg cattcccaag gcgtatattc
     4561 agggatgggg acagggactg gcactttcct tctttgcaac cttgttaatt tccgatgata
     4621 ttgagaagga tggtgcgtta tagatgtttc gcaggaattt cctgttcgga aaggatggag
     4681 gaacagccaa tctgatcgat gtgggcagcg aagacctcta tcagccgggc aaaggttacg
     4741 gttttgtaac agaaaaaaac agaagagagc agaagttgct gcagatccgg gaactgaatt
     4801 cctcttttga tacgatgtac tggtatcaaa acgaacagct gtccttcctg aaggaagatg
     4861 agaacggctg ctatctggat tctgcggaag aagtggcatc gttggagagg cagtcgggag
     4921 aaccgatgtc tggctcaccc aggaggattc cgctgatctt caaggtagat gtacccagac
     4981 aggggaacta caggatcacc ctgaccatcc gctcagaaga ggaaatggga gagatactga
     5041 tcttcaccgg aagaagacgg ctggcttttc atggcacagt gggagccggt gagtttgtct
     5101 ataccatgac ggtaaatgtc tgtgatatag taccgtcggg acagacccat atttttgcgg
     5161 ataagaccgt agatattgca gtattggcag acagaccacg gatcagtgct cttacggtag
     5221 aagaggtgaa cggtcctacg gtttatctgg cgggagattc cacagtgaca gatcagcccg
     5281 gagattatcc ctattatcca ggcacctgtt actgtggctg gggacagatg cttccggcat
     5341 attttgacac acgtgtggca gtgtcgaatc attcccactc cggactgact accgatagct
     5401 tccgcaagga agggcattat gcggtgatct ctcagtacag caagccggga gattatgtgt
     5461 ttttccagtt cgggcataat gatcagaagc ttcccgggct gcaggcaaag ggcggatacc
     5521 gggcgaatct gcaacgctat atcaaagaaa atcaggcaaa gggggtctat ccggtattgg
     5581 tgacacccat tgcaagaaat acctggagac tgcgggatca gacttatctg gatctgctgg
     5641 aggaattcgc ggatgtatgt ctggaactgg gagcacagta cggaattccg gtgctggatc
     5701 ttcatgccca cagcaagaaa tatgtgttgg aaaagggctt acaggatgcc aaacccatct
     5761 ttttccccgg ggactatacc cataccaatg acttcggtgc ttataagatg gcaggatatg
     5821 tggcacagga gatccgggag aaatgcaaag gtcattctga aagagcctac gcttatctgg
     5881 cagagtgcgt gacggatggt ttcggcgcat gggagcctgt gggacaaatc aatgttccta
     5941 agaaaccgga gatctacaag gacattccgg atcctgcagg agatcaggtg ctgttgtctg
     6001 aggcagagca gctggaacgt gtggtcagac tgtgtctgaa agaggaattg ctgtaacagg
     6061 acagatattg tactggaaaa tagaagtgaa gaaatgacag gaatgaaaaa taagcaggaa
     6121 tatgaggagg aactcagaat gggaaaaggt ggttttacct taccgggaga atcaggatat
     6181 gaagaactga ctctgaaaat ggcggaaaaa tggggagcag atgtgatccg tgacagtgat
     6241 ggaaccgtgc tttccgacga aatcacccat gccggttacg gaatctattc taccatttgt
     6301 attatcagag atcacaatgc ctgggcaaag gagaatcagg acaaattaca gcagaccttc
     6361 ctgtgcacac cgccccaggc agcagagagt gacaccctga ccatcggact gatggacagc
     6421 ttttttgcgg agcagttccg gatcaatgat agcgcagaga gtctggaata ttgggaggtt
     6481 tatgacagaa ccaccaatgt gaagatcgac aatgcggact ggagctatga caaagagaag
     6541 ggaagcgtgt ctctgagcgg catcatccct tttcataagt atacagtaag cttcttagcc
     6601 tatcgtatct gggaagaaat ctctatgtac aatcatacta cgaaccactg ggacaaggag
     6661 catctgatgc agatcgatcc cagatatcct gagactcagg agtatctgct gggctggatg
     6721 aagcactggt gtgagaccca tccggatacc acggtggtgc gctttacctc catgttctac
     6781 aatttcgtat ggatctgggg cagcagtgag aggaaccgga atctgttctc tgactggggt
     6841 tcctatgatt tcactgtgag cgttcctgct ttacaggagt tcgagaaaca atacggatat
     6901 cgcatgacag cggaagattt tatcaataag ggacagctgc atgtgactca tatgcctggc
     6961 aatgcgaaaa aggcggattg gatggcattt atcaatgatt tcgttatttc cttcggcagg
     7021 aagctgatcg atatggtgca ttcttacggc aaaaaggctt atgtcttcta tgacgacagc
     7081 tgggtaggcg tggagcctta taatggcaga tttcaggagt ttggttttga cggactgatc
     7141 aaatgtgtat tctccggtta tgaagccaga ctgtgtgcgg gcgtggatgt gcctacacat
     7201 gaactgcgtc tgcaccccta tctgttcccg gtggggctgg gcggtgctcc gactttcatg
     7261 gagggcggca atcccacact ggatgcgaaa aaatactgga acagcgtaag acgtgccctg
     7321 ctgcgtgcga agatcgaccg gatcggtctc ggcggatacc ttcatctggt ggagggtttc
     7381 ccggatttct gtgattatat tgaaaaagtg gcagatgagt tccgtctgat ccgcagcttc
     7441 cacgatgcgg gagaacctta ccgcatcaag acccgtgtgg cagtgctgca ttattgggga
     7501 agcttacgtt cctggacctt gtccggacac tttcatgaga cttatatgca tgacctgatc
     7561 cacatcaacg aagctttgtc cggacttcct gtggatgtga aatttatttc ctttgaggat
     7621 gtaaaaaatg gtatactgaa ggatgtagat gtcgtaatca atgcaggtag agccggaagc
     7681 gcctggagcg gcggggatgc ctggaaggat gaggagcttg ttactgcact tacaaagtgg
     7741 gtacacgagg gaggctgctt tatcggtgtg aacgagcctt ccgctgtgga aggatatgat
     7801 acgtatttcc gtatggcaca tgtgctggga attgacgagg acaccggcgc aagagtctgt
     7861 cacggcagat ggatctttga gacagcggat ccggagcatc tgatcccaga gggcgcaggt
     7921 gtggaagcga aggagaaccg gtatctgacg gatggaaaag cacaggtact tctggcagac
     7981 ggcgggaagc cactgatcac cctgaatcat ttcggcaagg gactgggaat ctatctgtct
     8041 tccttccagg tgaacctgtg gaataccaga atgctgtacc agctgatccg ttatgccggt
     8101 ggagaaggca cctccggcag ctatatgacg gataacctgt atacggaatg tgcttattac
     8161 ccggagagta aaaaactggt agtcatcaac aacagtgaca ccgagcagac caccactatc
     8221 ccaacggaag cgggcgcatg tacggtaacc attgcacctt acgacactac ctttgtagaa
     8281 atcagataac caacgataac gatcagggcg gaaatcctgt gttcgttttc tgagggagca
     8341 gaacgcaaag gaggcagaga atgcataacg aaaaagaaag acctacggaa tatttcccgg
     8401 acggaactcc catagatgac tggttttatg atatagggat gccggagctt gaggaactgg
     8461 gcagacccta tgtcctcaca gagtacggga tctgggacga cggaaaagtt tataccaaag
     8521 agatccagaa ggtgatcgac ctggcggcag aagagggcgg tggtgtcctg gtggtgccgc
     8581 cgggaaccta tctgacggga gcactttatt tcaaacaggg agtgaatctc tatgtttccg
     8641 ccggtgcgac gttaaagggc agcgatgata tctcggatta tgatctggcc gagactcgga
     8701 ttgagggaga gacctgcctg tattttacgg cactgatcaa tgcggacggt ctgaatggat
     8761 ttaccatgtg cggaccgggc actattgacg gtaacggact taagacctgg aaagcctgct
     8821 ggctgcgctg gaaatggagt aacggcacca acaaggatgg tcagagaccc cggctggtct
     8881 atctgtccaa ctgtagaaat gtgcggatcg ccggtctgaa tttacagaat tcccatttct
     8941 ggaccacaca tatttataaa tgtgaccatg taaaatactt gaactgccat atttattccc
     9001 cggcaggttc tgttgcggca cccagtacgg atgccattga tatcgatgtg tgtacggatg
     9061 tgttggtgaa aggctgttat atggaagtca atgacgactc tgtggtgctg aagggcggta
     9121 agggcccctg ggcggacagc cagccggaga acggctccaa tgagcggatt ctggtggagg
     9181 attgtgtcta tggcttctgc catgggtgtc tcacctgcgg ttcggaatca gtacataacc
     9241 gcaatatcat catgcgcagg attcagatca agggggcagc gagactgttg tggctgaaaa
     9301 tgcggccgga caccccgcag cactatgaat atatcacggt ggagcagatc gaaggccggg
     9361 taaaggagtt cctcaagatt tatccctgga cccagtatta tgatctgaag gatcggaagg
     9421 accaaccgct gtcttatgcg gagcatatca cgatgaagga ctgtgactgc atctgtgata
     9481 ccttttttga tgtgatgccg gatgagagcc agtatctgct ttccgatttt accctgcagg
     9541 atctgcggat cagggcaaga gtggataact tttcggaaga caagttaaga agtgtagtat
     9601 atgagaatgt acaggtggaa gaaagtacgg agcctgcgga
//
LOCUS       DAWEJW010000103         9433 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2456_length_9433_cov_65.130625, whole genome shotgun sequence.
ACCESSION   DAWEJW010000103 DAWEJW010000000
VERSION     DAWEJW010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2456_length_9433_cov_65.130625"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7254..7353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2097 a         2718 c         1930 g         2588 t
ORIGIN      
        1 ttgtgaccgc ttatgtaccg ctacgctctg cgacaggagc gcatcgtact aaagtgcgag
       61 cgtgccgcgt ttcttatacc ttgccaaggg ctacggggat ttgatagcat ttctttacta
      121 actttccatc tatcttcagc cagtctctcc ccaccttgca tggcaaaaac aacaccttca
      181 ccccagcggc ctcggcttcc gccagagcgg taccaaattc cggctgcatt tccacataag
      241 gacgtacttc cgtcactcct tccatctgaa tcacaaaggc aaccacacat tcatatcctt
      301 cctgtctcgc tgcggccagt tcccgcagat gcttcacccc acgctccgtg ggtgcatcgg
      361 gaaaatagcc gatgccgttg atctccagag tacagccttt cacttccatg agtattttgt
      421 gctccccttt ttccagatag aaatcaatgc gggaatttcc ataggtatac tccggtctga
      481 ccacatcata gtcctgcgcc tccagccatt ccttcacgac cttattcggt gcctgactgt
      541 caatgttgat ccagccgatc ctgtctttat aaacagccac caggtcatac aatgtctttc
      601 ttgccggatt atccgactgt tccagacgca ccgctgcccc agggagcaaa agttccctgc
      661 atcttccggt atttttcaca tgcaccgtct ccgctttccc tgcaatctcc acatgggcaa
      721 taaaccggtt gggacggtca atgaattttc cctctacgat ctttccatac ttcatgtaaa
      781 atttcctatc cttaactcta tccgctcccc gactttcgac actgctttac gcccccaatt
      841 gccaagactc tgcggcattt cccgaggtct cccgcgaccg tccgccacat gctcattcta
      901 tccgcactcg ccgttccgca tagacttcac tagcagcggc acctcgaaca acagcatcac
      961 gatccatccg accatattgg cccaggccag gctggcaaat tccatctgta acagacggat
     1021 cattacaaat actgccacaa ctctgccgaa catattcata aatgtgctgt acaatgtcac
     1081 tttcagatca ccaataccac ggaagaatcc ctgcacacca ttggtcaatc ccggcagaat
     1141 atacatccag gagatcagat gcagataact gatgcccaga gataccacct gtgcatcccc
     1201 gtcctcggcg aacaatgtca tgatctgcgg agccagtccc cagatcacac agaacaatcc
     1261 gatggaatag accagttcga tcaaaagacc tgtctggaat ccctttttca tccgctcctt
     1321 atgccctgca cccttgttct gtgccagaaa agtcgtcatg gcatgtccga tattctgctg
     1381 tggtgtatag gcaaaatcat ccactcggtt caccgcggta aatgccgcca tgaccgcaac
     1441 accctgcgta ttgaccacag actgtatgat cagttttccc agctgcagac atacctgctg
     1501 catggcactg gtactgccgt agctgactgt cttgcccagc aggctcttat cgaataccca
     1561 ccactttttc ccaagacata aaagaggaat ctttttctta atatatatac cgcacagcag
     1621 gcagcaaagt ccctcgctgc acactgtggc aatggcactt cccgccacac cccagcgcag
     1681 taccacaacg aaaaacagat ctccgaagat attcaggaaa gcactggtca ccagaaaata
     1741 cagcggcgtc ttgctgtctc ccagcgcccg catggtattc gccagaaaat tatacatgaa
     1801 cgtaaagatc aggcccatga aaatgatcct aagatatgcc gcagcttcgg gtatggctgt
     1861 ctccggcacc cgaatgagcc gaagcaccgg attcgccaga agaagcatca gcagtgaaaa
     1921 cactacggaa aacccgcagc cagctaacat cgtcgtactg atctgtctgg ataaagtatc
     1981 ataatccttc gctccgtact gggagctcat caacacactg gctcccatgc acatcccgct
     2041 gatcaacagg attgcaatat tcataatagg agtggaagtt ccaactgccg ccagtgcctg
     2101 ttctcctaca tattttccca caatgacgga gtccgctgca ttgtaggtca gctgaaacag
     2161 attccccagt accaacggga ttgtaaaaga tactaagggt aacagtatct ttccctgggt
     2221 catgtcgtgt ttctctatcg tactcattgc tttattgtcc tttcactgtc attttcttcg
     2281 gtaattgtct ttccttcttt ttaagaattc catcctcttc tggggccgcc tctgtactgt
     2341 cctgtgaact ttcattaccg ctgacagata acgcgctctc cagtccgctc tgcttctgcc
     2401 cgtctatgct gttcccggat gctgcctgct gtccgctgtc tgtcgaagca gagtctgtca
     2461 tttcagccat attggcacta tcctccgcag cctcaccctc cggcatagca gcctcctctt
     2521 ccgccattgc cgcctctttc tggatctcgt tctctgttcc tgctgtatcc cccgaccggg
     2581 cagcatccac cgcctcttcc tggatctcct gatcctcagt gataccgtag gccatctgtg
     2641 gtgctgccgt atccgcattg ccggtattgt ctgccatttt ggtactgctc attgtcatat
     2701 acaatactcc cgctaccacg aaacacaggc aggctgccgc aatgcgggtc acatagcgca
     2761 tcgggaactt atgtaccctt cccgaatttc tgatctgtcc gcttcgaagc aacagatcct
     2821 catccactcc agacatcgcc cggaacagtt tttcttcttt taaattcaca tcttgattat
     2881 tgttcttatt acaatcgctc atatccttca tcaccgcttt cgtctctttc tccgtctata
     2941 agacgattcc ctgttgttcc agatattttt gcagtttttt tctggtccgg aacagcgatg
     3001 tcttcaccgt attcagattc attccataac gcccggcgat ctccgcatat tcctctacga
     3061 accagtatct gcgtaggaat acattgcact cctgttccga aagtgtatgc aggaacgcat
     3121 tgatgctccg ctccagttcc gccgcttcca cggcttcttc tgtgttatgc gccgccggaa
     3181 tgcactctgc cagttcatcc agtgtcgtat ccataatacc attcccacgt ttctgtgcat
     3241 ttttcgcctt ccatctatca aacgacaagt tccgggtaat agttcccaga aacagctcca
     3301 gacgattcgg ttttttcggt ggaatcgcat tccaagcccg catccaggta tcattcagac
     3361 attcttcggt atcttctctg tcatgcagga tatgataagc aatactatag cagtattttc
     3421 cgtagctctt ttgtgtctct gcgatggcct gttcgtccct cgcccaatac agttccaata
     3481 ttgcggtatc ctccattggc tgtctccttt ccaaaaaggg cactgccaca gttttttctg
     3541 taacagtgcc cctcttcatt tctccatttg ctccgttcca tcttatccga gctggaattt
     3601 cttcgcattt gcattcagtg aacccgcaat ttcattcagc tgtgtggtag cctgtcggca
     3661 atcctccacg atctccgcca gctgatccat ggtagctgcg gtctcttcgg tgcttgccgc
     3721 attgttctgg gagatctcgg taagtccgtc gatcttctcc accacatccg tcttataatt
     3781 ctcaatatga tccagctcgc cggagatcac attgatctca ccgactacct tctgaacctc
     3841 ctggttcagg gtgctgaaga cattcagcgt ggtacccagc ttctcgttct gctgatggat
     3901 ctcacctacc acaccgttca tgatctgaac gctctggttg gaattgctga tcaggttctc
     3961 cacgatgctg cggatcttgt ctgcggattc cctggactgg tctgccagac cacggatctc
     4021 ttccgctacg actgcaaagc ctctgcccat ctcaccggct ctcgctgcct caatactggc
     4081 atttaatgac agcaggttgg tctggttcgc aatacctgcg atgatatcgg tagctgcctg
     4141 aatcgcctgt gcggactcgt tggtgatatt ggtctgctcc tgcacctgat ccaccgactg
     4201 ctgtgtatga gagctgatct ccaacagttc cttcagggtg ctgtccacgg tagcattgct
     4261 ctccttcatg ttggcagcac tgctgctcaa agcattaaca ctgtctgccg tacggcccag
     4321 tgcattgctc atctcgttca tgctttcgct gactttctgg gtatccgccg cctgtgtggt
     4381 agctccctgg gcaatctcat tcacagctgt atttacagaa gaaatggact gtccgatgct
     4441 gtcaaaattg ctggtgaacg ttcccgtaaa ctcattcata tccagcatgg aattgtgaat
     4501 atccctgata attccggaaa agccgttcac tacagagtat acagcccgtg cgctcttacc
     4561 tacttcatca ctgcggttta acagcttatc gggaaccttg aagttcaatt taccctctgc
     4621 gatctgatcc agattaccta ctacacccag tagtgctttg gagatcatgc ctactacaat
     4681 aaaggtggag acacagaatg ctgccacaag gatcagaatc gctattgctc ttcctgttac
     4741 aatccctttg taaagcgcat tcacttccct taccggcata caggcagcca gaatacccac
     4801 aaccgtgccg tcggtattct tcaaaggtgc cagatatgca tagcatccgg tatcccccag
     4861 cttcagggaa ttggaaaaat agctttctcc acccagtacc ttggaagcca ctttttcact
     4921 cagagtgatg gtacctccgg tcagattggt cacagccaga tcactgcccc agaacagtgc
     4981 cacatccaca ccggcattct gtttcaaagc atcgagtacc tgcttattgt ccgtaagatt
     5041 cagttcccct ttataaagtg ctccgttctc gtatgaaaag tctccgggag cggaattgtt
     5101 caggctcatt tccagtgcat attccgtcgc tgccagctca tgcttcatca tcgcgtcgga
     5161 aattctgtcc cctgcggaat tcaatgccag gatggcaatg actgcgatcg ctataagcgg
     5221 taccagtacg atcgccagga tcttgactac catgttagct ccctttttct ccttcaggcg
     5281 cttagcttca tcaatacctg tcccattttc ccctttgatc tgttctttgc ttacctcttc
     5341 tgtcataaat aaagcaacct cctttgtacc tttaacggtt catgcataat agaattatac
     5401 cgcacagtta ataaaaaagc tattcttttt ctgcaatcag atccttgatc acttcgcctg
     5461 cgagaatcaa gcccgctaca gacggtacaa atgaaatact gccggggatg gctcttctgt
     5521 ccgtgcattt atgagtggta cccggagggc agacacagcc tgtcttgcag ctgttttcca
     5581 tattttccag cggcttcacc gggctttccg tagaatacac caccttcagc ttcttcacac
     5641 cgcgcttttt gagttctctg cgcatgacct tcgccaaagg gcacatggta gtcttgtaga
     5701 tatccgccac atggaactgc gtggggtcta atttgttgcc ggctcccata cagctgataa
     5761 cgggaactcc cgcctccttc gcccgcatga tgatctccag cttggcagtc acggtatcca
     5821 ccgcatccac cacgtaatca tactgggtaa aatcaaactg gtctgccgtc tccgggagat
     5881 aaaaacattt gtgtactgta acctgtgcct ccgggttgat ggagtgtaca cgttcctcca
     5941 tgacatctac cttgtatttg ccgatggtct tcctggtagc gatgatctga cggttaatat
     6001 tcgtcagaca gaccttatcg tcatcgatca gatcaaaggc tcctacacca ctgcgggcca
     6061 gagcctccac cacataaccg ccgacaccgc cgatgccgaa gacagcaact cttgcgccgt
     6121 acaggcgctg catggcttcc ttgccgatca ataattctgt tctggaaaat tcatttaaca
     6181 taattcactg ttcctaccaa tctactggga atactctcga aagagtatac cacatcttta
     6241 cagattgtca tagagtccct gaataaaatt tttcacctgc acgttatttt ccggtatggt
     6301 gtcctccaga gtggcatgga taaagggctt gcgctccttc ataaacttca gtacagaagt
     6361 ataatccatc tgtcccagac cgcagcctac agaacgaagc ttcccgtcct cgggaataaa
     6421 gtctttcaga tgtaccatag ccacatcttc acccagcaga tcgattgctt catccacgat
     6481 agccacctga tccttgtaat tgcagtaatc cagcaggttc accggatcca gaatgatctg
     6541 aaggttggga gaattgatct catccagcac tcttctggct ctggcggggt tgtatacgat
     6601 atgcttccat accggttcga tggccatgac aacgcccatc tgctccgcgt attttactac
     6661 cgggcgtaag ttggtgataa aggtctgtaa tgcttcttct ccgtggcatt ccggtaccgc
     6721 ggtataggtc tcgttgggag caccggtctc agttcccacg acaccgcagc ccagccagga
     6781 agcaaaacgg atatgtgcca tataacggtg ggtaattttc gcaagctgct ccgggttggg
     6841 gtttgccagg ttcaaatagc atcctaatac cgcaacatcc acctgatttt ttgcgaatac
     6901 atttttcatg tacatggcaa gtcccggggt cagcgcctca tccgtggtgg gaaactcgct
     6961 gatcaccttg cccggagcca gatgtccaca ggcaaagccc agctcatgta cattggcaat
     7021 acgctcctca aaaggaagct ttttcgtatc atgtaaacgg attcctaact gcatattttc
     7081 ctccattccg aacacggctc ctgggccgta ttctcttctg cttatctcta ttatagcgtg
     7141 agctcacgaa ttccgtgcta tcgcactcta catcctgctt cgcagctatc atgatcagct
     7201 tcgccgttcg ccgagagtga gcaagctccc tctggctctc ttgcgctcat tatnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttcgcc gttcgccgag agtgagcaag
     7381 ctccctctgg ctctcttgcg ctcattatat caacgtctga tcagttcatc cacaccttcc
     7441 agttcgtaag cttctccgac ctgtccttca atcttcacat tgtccagtgt cagtttcgct
     7501 acatttctgg caaacattcc gcgcttgctg ctcttctcca cgccgtcaga catggcgggg
     7561 acatcacact tggcatcttc agcatagctg atggaaatgt ccttcatgta gatttcctcg
     7621 atcttctgct cgggaagtcc gtcgaagaac gcagctgcca catggcagtt cgtacattcc
     7681 atgttttcaa ataccatctt tttcacctgc ggtgttctgt catccacagg gtaaacatct
     7741 ctggactgga cataagtggt cttgccgtcg ggatcacaga aatagaagca gttgaccacc
     7801 agcggtgtca tcacatggtc cagcgtcaga tttcggaaaa tgatattgct gagaattgca
     7861 tcttttcctc tgccgcgacg ggtcttgata cgcagacctc tgtctgtatg acggaaaata
     7921 cagtcttcca ctgtcagatt taccacgccg cctgccattt cacttcccag agttacggca
     7981 ccgtggccgt tctccataag gcactgacgg atatggatat tctcggaagg agtcttatgc
     8041 tttttgccca tgtagatctt accgctcttg actgcaatac agtcatctcc cagggaaaaa
     8101 cgcacaccca ggatctccac atttttgcag gattccggat ccagtccgtc ggtgttgggg
     8161 gaatcgctgg gattctgcac ggtgacaccg atgaatttca gatcattgct gaaataggga
     8221 tgcagggtcc aggaagggga attgcagaag gtcacaccct gcaaagtcac atttttacat
     8281 ccgctgatga aaaacagtct gggacggaat gcaatattca taaccttcgg atccttccac
     8341 cagttttctt tggaagcatt gccgttgatc ttaccctgcc catagatcac cacatcctct
     8401 gcaccgatac cacagataat gccggagaac ataggtaaag gattgccctc ccaggtgccc
     8461 agattgtact cgtcggtctc atcatagctc tgcagcattc cggggaacgt agggaatttc
     8521 tctctgtcgg taaaggcctt cagctccgcg ccctctgcca gctcaatccg gatatggctc
     8581 tttaagaaca ggctggttat gcggtagatt cccgccggga aatacactct gctgttcacc
     8641 ggacatgcca tgatggcagc ctgaataaag tgggtatcgt catgttcccc gtcacccttg
     8701 gcaccgaatc tggtcacatc cagagtcacg tactcataat ccgtcttgaa agatacttcg
     8761 ccctgctttt ctccgttcac ataagcttcc accacatatt tctgatccgg cttaaggcta
     8821 aacagggaag ttatagtctt ctctgtggtc agtgacggct caccgtttac ccgcagctcc
     8881 acgggctgtt ttgtaaaata tctgccgccg tctgcgatct ctatggtcgc actgcgggca
     8941 gtatgcataa tatgtctgat ttccatgcgc tgtttcctct cgttctctac ctaatgcact
     9001 cctgatattt ttccgctaac aataaatact ccgcacatgc cttctgtacg gcatcgcaga
     9061 ataccttttc cgatgtggca tccatggcaa acttattttc ctttgcatca taatactgct
     9121 ccagcatacg ctttaccgca ctgcggaaat tgtccagaag ctctctgtaa ttctcgtaga
     9181 tctccgggct gatctgctct acggtatgaa gcatgactgc ggtataagct gcaccgtttt
     9241 cagcactgaa ttcactttct gcatgctttt tcagcacacg catctgtgcc atgatatccg
     9301 gatagccttc ttttttattc tccgtggtct cataggccgc atagacggga tacaccagat
     9361 ccgccgcctg ataaatgttg cccagacctg cactctgctg cacggctgcc aggtcgaaaa
     9421 tgccttcctc cgt
//
LOCUS       DAWEJW010000104         9292 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2496_length_9292_cov_56.846487, whole genome shotgun sequence.
ACCESSION   DAWEJW010000104 DAWEJW010000000
VERSION     DAWEJW010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2496_length_9292_cov_56.846487"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1084..1183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2785..2884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1958 a         2582 c         1928 g         2624 t
ORIGIN      
        1 agataatcga tcagagatac tacggtgagg atcagtgcga tccacataat aatatcggtg
       61 aggatctgta actgcgggaa gttggcgatc atcagacaga tcataaccat ctggaaggtg
      121 gtcttgaact tgccccagta gcttgcggcg atgactacac cgttgtcgga agcgatcaga
      181 cggaagccgc tgatgatgaa ctctctggcg atgatgatga tgaccaccca ggaagggata
      241 cggttcatct cgatcagtgc gatcatggcg gaacatacta acagcttatc cgccagcgga
      301 tccatgaact ttccgaaatc tgtgatcaga ttgtattttc tggcgatctt accgtcgatc
      361 agatccgtca gactggcgat gatgaagata gccagtgcga tcatatccgt cacgaaggta
      421 ttatcgccga accatccggc ctggcctcca agcaacagga taataaacgg cacgatcagg
      481 atcacacgga aaatagtcag cttgttaggc agattcatct tactcatggt atatctctcc
      541 tattagatca tattcattgg aatcggtgac cagaaccttg acaaaatctc cggtcatcag
      601 attcgctgaa gtatttacaa acagatagcc atccacatta ggggcatctc tgtaggttct
      661 tgcgacatag gtctcttcgt ccgctacctt gccctcgatc attacagtca ggatacgacc
      721 taccatatcc tccgctttgt caaaagcgat ggcctgctgc agttccatca gttcatccct
      781 gcgaaattcc ttgacctcct gcggcacctg atcttccatg gtggctgcgg gagtatcttc
      841 ctcctgggaa taagggaata cgcccagacg atcgaattcc atctcgttaa caaagcgata
      901 cagttcctcg aaatcctcct gagtctctcc ggggaatccg ctgatcagag tcgttctcaa
      961 acagatatcg ggaatcttct cccgaagctt accgatgata gcacgaagtt ctgcctggtc
     1021 ggtacgtctt cccatgcgct ttaacacatt gtcggaagca tgctgaatgg gaatatccag
     1081 atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaaaatg tccgcctgta
     1201 gtgacaacac ggttggtctc tgtcacaggg gcggcattga tatcttcata atgattctgt
     1261 ttctggcctg ccagaatctc ttccagagcg gccacgatct catcgatggc agtggttccg
     1321 ataatggcat ccacctgcgg gatctccttc tggatttcct cacggtaacg ctgtgccaga
     1381 caacctgctg cgatcagtgc tttgacctgt ccgctgtcct tcagttctcc catctgcagt
     1441 aatgtattga tgctttcttc cttggcatca ccgataaagc agcaggtgtt tactactact
     1501 acatctgcct ctgcttcatc gtccgtgaag gtataacctt tttcctggag catgccaagc
     1561 atcttctcgg aatcgacaag attcttgtca cagcccaggg atacaaacaa tatcttcatg
     1621 cgtcttatgc ttcctcatca tcaccaagga ttttaatctt accctggttc agctcttcta
     1681 ctgcgataga cagaggctta tccaccttgg cagttaccag aggctcgtca cctgcgatga
     1741 tctgtcttgc tctcttggca gttgccataa cgatagaata acggctgttg actacaggtg
     1801 cttcaccggg ttctacctcg ctgtttacta ctttcattaa atcggtataa gagggatgta
     1861 acattttatt ttgctccttt cgcgaaacct ttcagttcct ctctgatctc tttgataaat
     1921 tctctacgac gggacggtac atttttcgct gcttccacat acttctgtac ttctgcggca
     1981 cacacatcca gatcatcgtt cactactata taatcatagg catccatgcc ttctgattcc
     2041 tcataagccc gggacagtct ctgtgcaatg acatctgctg tctcggttcc tctgcccacc
     2101 agtctcttct gcagttcttc tgcactggga ggggtaacaa acagtaacag gctctccggg
     2161 aactgctctt tgatcttcat ggctccctgg atctcgatct ctaagatcac atccttgcct
     2221 gcctgcatct gctcttccac atatgctttc ggtgttccgt aataattgcc gcaatactga
     2281 gcatactcga taaatccgtt ttttgcaata ttttcttcaa attgttctct ggtacggaag
     2341 aaatattctc ttccgtcctc ctcgccttct ctgggctgtc tggtagtcat ggaaatggac
     2401 aacgcataat tgtcatattc cgataacagc tttttcacca gtgttccttt gccggctccg
     2461 gagaatccgg agatcaccat caatattccc ttattctgca tcttctctgt cctctttttc
     2521 ctcttccggc cacagtgcct gtgctcttcc cgcgattgtc tcgggaagca gtgccgacag
     2581 aacgacctga ctgttctcca tgaccagtac cgctttcgtc ttccgtccct gtgtggcgtc
     2641 gattgcctgt ccggtctcct tggccttctg caccagtcgt ttgatgggtg cggcctccgg
     2701 gctgacgatg gcgatgatct tatccgtatt tacaatattg ccaaatccta tatggatcaa
     2761 tcggttcatg gtatcacctt tcgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnttttcg atctcggtct tgagctcaat cgcacagttg gagatctcca gatcgttgga
     2941 cttggacagg atggtgttgg cttcgcggtt catctcctgt gccagaaaat ccagctttct
     3001 tccgatactg ccaccggcca tcagcgtact cttcgtggtc tcgatatggc tgcgcagtct
     3061 caccagttcc tcatctacgc ataccttatc tgcgaagatc gtcacctcgg tcagaagtct
     3121 tccttcatct acagtagtat cgcccagcag ttccttcaca cggtcttcca gcttctggcg
     3181 gtattcagca atgatctggg gagaacgttc cgtaatgaaa tccaccagct tcagcatgcc
     3241 atccagcttg ctaattaagt catctcttaa atgctcgccc tcggtgatcc tggtctgtac
     3301 aaacatctcg caggcaccct tcagggctct ctgcagttct ttccacagtt cttcctcatc
     3361 gatgtcctgt tcttccatca caaatacatc cggatacttg gacaaagtgg acactcggat
     3421 atcgcagtct aagtcaaaat cactggccat ctgcttcagg tatcccagat actccgccgc
     3481 cagttccttg ttgtaacgga cattggacgt gttctcggaa aaatcctcgt aggtaatgaa
     3541 cagatctact ttacctctgg aaatataatt cttcagttca ctgcggatcg cagattcaaa
     3601 gaaattcaac ttcttcggca ttttgatgtt cacatcaagg tagcgatgat tcacagactt
     3661 catctctacg gtaaactttc tgttattctc ggcagtttca catctgccaa atcccgtcat
     3721 actcttgatc atagcttctc ctgttgtaag ggcgtgtaat accttccgca atttacggtt
     3781 ttcattctat tatagaataa atatgccctt tccgtcaagt gcaggtgccc tatttacact
     3841 atttccactc gtcgtaatgc tccagaaaat gatgcttcac accatcctct ttgtaggcga
     3901 tgatcgtacg taagtttaca ttctccacac tcttgaaaat attcccctcg ataaaagggt
     3961 tagtctgttt cagcgaagcg atcaggttct taatctccat acgcttggag ggactgctgc
     4021 catcggtacg acaagtggta caagtgtccg tgaaccggca ggcacactga ataaaatgca
     4081 aatcattgta atctctccag tgcttgatgt catcctccct gatcaggtac atagggcgga
     4141 tcagctccat gccctcgaaa ttcgtactgt gaagcttcgg catcatggtc tgtacttgtc
     4201 caccgtacag cattcccatc aggatcgtct cgatgacgtc atcgtaatga tgtcccagag
     4261 cgatcttgtt gcagcccagc tctttcgcat gactgtacag atagcctctg cgcattctcg
     4321 cacacagata gcagggggaa tcctctatgt catacaccgc atcaaatatc tgggattcaa
     4381 aaatggtgat gggaatatcc atcagcttcg cattgttctc aatgatctta cggtttgcct
     4441 cgctgtagcc gggatccatc actaaaaata ccagttcaaa ggggaacttg ttatggcgtt
     4501 tcagctcctg gaacagtttt gccatcagca tggaatcttt accgccggaa atgcatacag
     4561 cgatcttgtc gttttcctga atcatatcgt attcgttgat agctttggca aaccgggaaa
     4621 acaggacttt gtggaacttt ttgcggatgc tcttctcaat ttcccttgcc cgttcatttt
     4681 ccttttctcc gggctgttcc ttctgcatca ggttcagcag ccatccggta tagccgccct
     4741 gtaagctgta gacctccatt ccctgttctc tgaaatactc tgcggacacc ttactgttct
     4801 gtccacggct gcaatacaaa attacttttt tatcccctgc tgccgccgcc agtctgtcta
     4861 taagttcaca ttcggggatt gcaatggctc cgggaatcat tccatagttt actgcttcat
     4921 cccctctggt atccaccagg atgtatcggt caaggtcccg ggactgtagt tcttcccatg
     4981 tgatctcaat gggctgtatt gtctcttttt cttccatttt tatattcctt tactctgccc
     5041 gaacaggcag tctattgttt ttcctctctc agtctgctac actgttatta aaaatatggt
     5101 aactgaatcg ttacattaca tccatagaaa ggcacacaaa tgattcctta tactacttta
     5161 gattccatta tagctcaaat gcccgaacgc accgggcaca ctctgttaga tgaagaccag
     5221 gggtgtgtaa aagggtgtcg tatgggcgta tccctctatc gcaacacaga atatcacatg
     5281 ccgcaaaagc atgacgatca ggaaggcttc ttcgtattgg acggaaaagg atatgctctc
     5341 atcgatggcg aagaaatccc tctgctgccc ggtaccgcct ttatggtacc ggctcacacg
     5401 gatcatgtga tcagacggga tccctcctgt gactgctgta aggttctatg gttccatgcc
     5461 gcagtagaat gatcctcagt ccccacatac cgccagaagc tccgtcagct gcgcctgaag
     5521 ctctgtcagg ctgtcacact ttttgtctac cagcgcaaga atctcttctg tgggcggcag
     5581 ctggtccggc accatcacag tatataagcc tgccgcatag gcggaccgga taccgttgaa
     5641 ggaatcctcc accgccagtg cctgtccggg tgcaacccca agctcctcgc atgccttcag
     5701 ataaatatcg ggtgcaggct tgccatgctc caccatgtcg ccgcagataa ttctctgaaa
     5761 ataagatgtg atacccgctc tgtccagatg ctccataact gcggtcctgc gtgtagagga
     5821 tgccaatgct gttggaatgc ggttctcctt cagatattcc aggatctcat gcacgcccgg
     5881 cttctcggga aggccttccc gttcttcctt ttccttaaat ctccggctgc aatcctcatg
     5941 caattcttca aaagacacat cgatgccctg ctctgctttc gctgcctcga acacttcctt
     6001 cgtgccctgc agtgtccttc cgatacaggc tttggaaatc tcgtccacat ttttaaggcc
     6061 cctgggctcc gccgcttctc tccagcactc catatacatc ctctcggtat caaagaggac
     6121 accgtccata tcaaatatca ctgcggttat tgttgtcttc ataattctcc tcacatccag
     6181 agcaccatca gattgttacc cataccgatg atccccatca ttttgcgcgt aaatagtaat
     6241 cgatgatatc tgtctcttcc atggaaatat aagttccgta catggctatg ttgcagcaca
     6301 ataccctgct tcccgcaggg aagatcatct catatccttt agacgaagcc agccccggat
     6361 atctgtcatc cgctcttccg taggaaaaat aagcagcgga attacctatc ttcaaaggtt
     6421 tcggtgcccc aatccctccg gcgatcagag cctcgtcctt tttccattca aaaaagctcc
     6481 agttcttttt ctctccgagt tgccttggtt ctctgcttcg ttccgtcagc agaatcttac
     6541 cctgtgtatt taaaaatgtc aatgctcccg tctttttatc cacacggatc tgagcatatc
     6601 ccgctgtaat ttccaccagg gaaggattct cccgcaccgt gcatctgagg tctcctgctg
     6661 ccgtcaccgc cgctttcggg aattcacgac actgcccttt ggcaaaacaa atccggaaca
     6721 gatccaccgc aaggggtgtg atccgcaagg ttccgtaact actcaaaaga tccacatagc
     6781 ccttgccctt catgaccatg cgcacagcgc agtcgatcct ggaatgtccg gcgggcatca
     6841 gtgttttggc atccggcata tcgccgtact cgccgttatt ttctaccatc cgcggctcct
     6901 gtctgccggt tgctctgcca gcattgccgt ttcctgcccc gtatactttc gcaggtgacg
     6961 gctgtcccgt ccgcctcgat tcaggtcccc ctgactgtcc tgctttcttc ggtgtagcgc
     7021 cgtctgttgt cccctgactg tttcttgtca cgatgatctt ctttgggccg taacgctctc
     7081 tggccacacg ttccttttcc gcttcctgtg ccctctgtct gtagatctct tcttttgtct
     7141 tctccggctc tgcctgtttt accaccgttt tcaccggctt cttctgggca tctgcggcaa
     7201 ggcgcattct ctgcttctgc tctggggata ccggatctac gatcagtccg gtgagtttcc
     7261 cccggtacag caccgtcagt tcatgcaggg ctcgggtggc tgccacgtac agctgcttca
     7321 catacccgtc ttccaccggg taatcttcct ccgaagcgtt gaagatcagt actgcatcga
     7381 attccagacc tttggtgtat tttaacggaa gcaccatgac gccctcgccg atctcccatt
     7441 ttcccgcatc tccggtattc aggtccacgc tgccctgtaa ggctgctgtt actttttccg
     7501 cctccgcttc gtcaatgcag actacggcaa tggtatccag ccccttgctc tgccatgctt
     7561 tgatgatctg ctccgcctgt gtcacctgct ctgtaaaatc cacacatttt ttcaccgtga
     7621 ccggttcccc atgacgtatg atgggctcca ccggatagac cggaaagctt ccgtgataca
     7681 gaatgtccgt ggcaaactcc gagatttcca ccgtgttacg gtagcttttc tgcaaaatgc
     7741 cgaaataatc aaactctcca ggcagcatca atttccgcag ttccatccag tcgttgagac
     7801 cgtagcggtc ggaaatattc tgggccacat ctcccatgat cgtataggta catttgctca
     7861 gacaatattt taaagacgca taggccatca tgccgaaatc ctgggcttca tcaatgacca
     7921 catgccccgc ctcccggatc accgtgtcct ccttgatgcg cttgtagagg taggccatgg
     7981 ccgccagatc atagacatcg tagccacctt ccggcacctc gaccgtgaaa tctttctcct
     8041 gctcttcctt caggaactgt tcgtagagtt ccgccacaga gcccttccat tccctttttc
     8101 cgaaataggt ctcataataa tgcttgagct tctgcttttc cggctgggta taggaataat
     8161 attttccgct cagctcattt tccagccgtg ccatcagata atccgtcagt ctgctgattt
     8221 tgtccgcccg ggacagatcc tttgtctctt tcaataagcg tgcaatggtg gcccggtcca
     8281 gcagcacttt gccagtcttt tcgatcacca cgtcctctct gggtatcgcg cggtattcgt
     8341 aacgcaggca gaaattctcc agctccttaa accagacaag agttcccttc accgccgggg
     8401 tcacaccttt taccaccggc ttgatctgcc aggacttgtc ccagtcctcg tacaaaagcc
     8461 ttacgaacaa ctgctccatg gtcatctggg agacaccgta aacattcagt tccggcagca
     8521 ctcccgttat atagtttaag agcacctgat tgcttcccac aatataaaaa tcttccggtg
     8581 caaactcctg ctcgtagttg tacaggatgt aggaaatacg gtgcatggcc actgtggtct
     8641 tgccggagcc cgccgctccc tgtacgatca tgttgtgctg gggctttttg cggatgactt
     8701 cgttctgctc ctgctggatt gtggcaatga tctcgcccag caccgccttt ttgttccgcg
     8761 ccagatattt tgtcagcagt tcatcgttgg ccaccacatc cgaatcgtag aagtccttca
     8821 gctcatcatt ttcgatctca taggtgcgct tccgcgacag ggtgatgtcg tattttccct
     8881 ccccctttac ggaataagag acatccccca gagcggcatc gtaatatacg ctggcaatgg
     8941 gtgctctcca gtcgatgacc tccggttctg ccgggttatc cgcaatgaca cttctgccga
     9001 tatagtagac ctcgtctttt ttcagttttt tgtcccggaa atcgattctg ccaaagtagg
     9061 gctttttcct cgcctgttcc gcccggcgca gatcctgatt cacgaacttg aagcgggcat
     9121 ccgcattgtg ccacagggcc tgggcttcct tgtcgttctc gtcgaattcc tcctgcatgg
     9181 catgcagttc ttccgccaga gcctgtacac tctgcctgtt ggcatccacc ttgcgctgtg
     9241 cgatctctaa cgtatgctgt aatctttttt cctcatcctg cctcgtaatg cc
//
LOCUS       DAWEJW010000105         8613 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2693_length_8613_cov_54.366441, whole genome shotgun sequence.
ACCESSION   DAWEJW010000105 DAWEJW010000000
VERSION     DAWEJW010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8613
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2693_length_8613_cov_54.366441"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3230..3329
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2271 a         1866 c         2302 g         2074 t
ORIGIN      
        1 tccgagcaga acgatctggt acaggcatgg ttcgactttg tactgggtga cgaaggacag
       61 caggttgctt ccgaagttgg tctgatcaca gtaaaataat agccgacgca gaaaaaggaa
      121 aaaagcgatg aatagtggaa cacacgcttc cgtaatggga aacagaaaaa ataaagcggt
      181 cgtggaaact gtggcacaaa tagtatttac catttgtgcc ttcttcgccg tactggcggt
      241 agtttctatc accttatata tgatcatcag cggtaccccc gctattatga aagtaggtct
      301 gggagagatc ctgttcgaca cggaatggat gccaacagcg gcagatccga aattcggtat
      361 tctgtgggtt atcctgactt ccatcgtcgg tacgacgctg gctatcctgc tgggtgtgcc
      421 catcggcgtg ctgacggcgg tattcctggc agaggtggca cccaagcccc tggctgccat
      481 tgtaaagcct gcggtagaac tgttggcagg catcccttcc gttatctacg gactgttagg
      541 aatctactta ctgaatcctc tcatgtataa attagaaaga ctgatcttcc agggatccac
      601 gacacaccag ttcaccggcg gtgcaaacct gctgtcagcc gtgatcgtac tggcgatcat
      661 gatcctgccc actgtgatca atatcagtga gacttccatc cgggcggttc ctgcaggaat
      721 caaatcctcc tctctggccc tgggagcctc ccatgtacag actattttcc gctccatcct
      781 tccggcagca aaatccggta tcgtgacggc tatcgttctg ggagtgggac gtgccatcgg
      841 cgaggctatg gcgatcacac tggtatccgg tagcagcgtc aatgtgccgt tgccatttaa
      901 ctccgtgcga ttcctgacca cagctattgt cagcgagatg ggatattctt ccggactgca
      961 cagacaggtg ctgttcacca tcggactggt gctgtttgta tttattatga tcatcaatat
     1021 cgtactgaat aaaatactga aaaagggggc ggatgacaat gaataaggac accttatatg
     1081 taaaaagaaa acgccccaca gacatgcttt tgctggcagc catttatgtc tctgccacac
     1141 tggcggtggc acttttactg ggaattatcc tgtatgtctt cgtaaagggc atccgaaccg
     1201 ttaactgggc atttttaacg acggtgacca gtgccagaaa gcagaccacc ggtattgcgg
     1261 gcaacctgtt gaataccctg tacatcatcg tgatcactct gttgatcgcg acccccatcg
     1321 gcgtgggaag tgccatttac ctgaatgaat atgcaaaacc cggcaagctg gtctccatca
     1381 ttgagttcac cacggagaca ctgtccggta ttccttctat tattttcggc ctgttcggta
     1441 tgatcttttt cggagagact ttgggcttcg gctattcact gctcaccggt tcctttaccc
     1501 tgacattgat ggtactgccc ctgatcacaa gaaatacgca ggaagcattg cgcaccgtgc
     1561 cggacagtta tcgtaccggt gcgttgggaa tgggatccac gaaatggcat atgatccgga
     1621 cgatccttct gccgtcttcc atgccaggta tcgtcaccgg tatcattctg gcactgggac
     1681 gtatcgtggg agagtctgca gccctgttgt tcactgcggg aagcgactac aagctgccga
     1741 agttcaccgg agttttcgga gacaatctgg caaagctggc tcacaaggcc atggagtctg
     1801 gtggtaccct gaccatcgaa ttgtatttac agatgcagaa gggacagtat gataatgctt
     1861 tcggtatcgg ttgtgttctg atcattatcg tattgatcct gaacctcctg accaagctgg
     1921 cggcgaataa gcttagacga gagtaagaga aaacacaaga aggagaaaac atatggctaa
     1981 cagtaaaata tccgtggaaa acttaaatct ccattatgga gagaaccacg cactgaaaga
     2041 tgtaaatatg gagatcgcag accacgccat taccgcattt atcggtccca gcggctgcgg
     2101 caaatctact tttctacgtt gcctgaaccg gatgaatgac ctggtggacg gctgcagagt
     2161 cgaaggaaaa gtaattctgg atggagagga catttacgat aagcgggtgg ataccacact
     2221 gcttcgtaaa aaggtcggca tggtattcca gcagcccaac ccttttccca tgtccattta
     2281 tgacaacatc gcctacggtc ccagactgca cggcatcaag aataaaaagg aactggatga
     2341 gatcgtggag cgttccctgc agggggccgc tatctttgaa gaggtaaagg atcgtctgca
     2401 caaatctgca ctgggtctgt ccggcggaca gcagcagaga ctgtgtattg ccagagcact
     2461 ggcagtatct ccggaggtac ttttgatgga tgaacccacc tccgcactgg acccgatctc
     2521 cacgctaaag atcgaggaac tgatggagac actgaagaaa aaatacaccg tggtcatcgt
     2581 aacccacaat atgcagcagg cagcccgtgt gtcggatgac accgcatttt tcctggtagg
     2641 ggaagtcata gagaaaaatg caacctccga gatctttgca agacctcagg acaaacggac
     2701 agaagactac atcaccggac ggtttggatg attttggatg agaagatagg aggaatggga
     2761 gatgtcaccc agagtattat ttgaacagga tcttgagact ttgaaaaata aagtatctga
     2821 gatgggagag catgcggaga tcagttatga ccgtatggtg tatggaattc gtgagaacaa
     2881 agaagacatt ctgaagacac tgttgaatac agatcatacc atggtggata tgcagcgaag
     2941 tatagaagct atgtgtctga gcctgctgac cagacagcag ccggtggcca gagatatgcg
     3001 tctggtatcc gcagcgctga aagtggtacc ggatattgaa cggatcggag accatgtggc
     3061 agatattgtg gaactgtacc tgcgtatggg caacatgaat cccgaaggaa aacaggaaca
     3121 tctgttgctg gagatgatgg aagaagccaa ggagatgatc cacaattcgg tggaggcatt
     3181 tgtggaaggt gatgaggcaa atgcaagaaa agtagtagaa catgatgatn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcatttgtgg aaggtgatga ggcaaatgca
     3361 agaaaagtag tagaacatga tgatgtggta gatgatctct tcaacgaagt caaaacggga
     3421 atgatgcagg ctatcagaga gcagagcctg gatgcggatc gtgtggtgga ttatctgatg
     3481 atggcaaagt atctggaaaa agtgggtgac catgcggtaa atatcgcaga gtgggccatc
     3541 ttccgcatga ccggagatat ggaaggtgcg agattgtatt aggattcgat aatctcggta
     3601 acgatgcaga gccccaattt gcaaaactgc tcctgcggag cgttcctttt gctcattgag
     3661 gcttgctcgc caaagaacta ttgccatata ctcttacgaa tgctagcatc tgataatccc
     3721 agtaacgatg cagagcccca attcgcaaaa ctgctcctgc ggagcgttcc ttttgctcat
     3781 tgaggcttgc tcgccaaaga actattgttc tatgctctta cagatgccag catctgacga
     3841 gcatagaaca atagttcatg gctctgcatc gttacaactt tacataaatt ttacattcac
     3901 tggtaacgca ttttacataa gcaagataaa gtgtagatag aaacccaaaa tgtaaagtat
     3961 gttatgtaaa atgagaggat tagagtatga gcttttttga tttactgaag atggtaggag
     4021 gacttgcatt attcctgtat ggaatgaatc tgttgggtga cggattgtcc cgggcatctg
     4081 gcggacgatt agaacgcatt ctggagaaac tgaccaacaa cccgttaaag gcagtgctca
     4141 tgggtgcggt ggtcaccggt gtgatccagt cctcctctgc taccactgtt atggtagtcg
     4201 gatttgtcaa ttccggtatt atgagtctga aacaggcagt aggtatcatc atgggtgcca
     4261 acatcggtac cacggtgacc tcctggatct taagtctgac gggcattgag agcgatgctt
     4321 tctggatccg tatgctcaag cccagttctt tttcaccgat tttagcgatc gtgggtgtgg
     4381 gtattttact gttttcccat aaagacaaat taaaaaatgc ggcaaccatc ctggtgggct
     4441 ttgccattct gatgtttggc atggattcca tgagcgcggc agtttcaccg ctggcagagg
     4501 taccggaatt taccagtctg ctgatcaagt tccagaatcc ggtcttcggc gtactggcag
     4561 gcatgatcct tacggcagtg atccagtcat cttccgcatc cgtaggtatc ctgcaggcat
     4621 tgtgcactac cggtgctgtc agctttggcg cagcagtgcc tatcatcatg ggacagaata
     4681 tcggtacctg tgtgaccgcc atgatctccg gtatcggtgc ttcccgcaat gcaaaacgtg
     4741 cggctctgat tcatttgtat tttaatatta tcggaacgat cctgttcctg ggagtgttct
     4801 atacggtcaa tgccattcat ccctttgctt ttctgggaga agtggcaaca ccttttggaa
     4861 tcgcagtagt tcacagctgc ttcaatatcg cggctacgct ggtattgctt cccttctcca
     4921 acggtctggt gaagctagcc tgcctgacgc tgccgaccca tgaggacgtg aaggaaatgt
     4981 ccgagacaga caagacttta agcttactgg atgtacgttt cctggagaag cctgcctatg
     5041 ccgtagagca gagcagactg gtggcttctg tgatggcgga taagtccagg gaagccatgg
     5101 atctggctat ggagctgctg cataatttcg atgaggcgaa agcagagcag gtggcacaga
     5161 tggagacgga tgtggaccgc ttcgaggatg aactgggaac ctatctggtg aaattgagtt
     5221 ccaaacagct ttccaataag gatagtgaga ctttaaatac gattctgcac tgcatcggcg
     5281 attttgagag aatttccgat catgcccgca acatcaagga agcggcacag gagatgcatg
     5341 acaagcagct ggtattctcc gagaaggcgc aggaggaact gaaggtattc gagagagcgg
     5401 tacacgatgt gctggatctt accatgacca gttttaagaa cggagatctg aatctggcaa
     5461 gacaggtgga gccgttggag gaagtcatcg atggactgag cctggatgtg aagcagcgcc
     5521 atgtacgcag actccgtaag ggaaaatgta ccattgaact gggcttcatc ttatctgatg
     5581 tcattacgaa ctttgagcgt gtcagcgacc actgctccaa tattgcagtc tgtctggtac
     5641 aggtgaatga ggatgaattc gatacccacg cttacctgga tgaactgcgc caggaagata
     5701 acaaggactt ccagagcaaa gtgctggcat gccgtgagaa ataccagtta ccgcccacaa
     5761 agcaggaacg tcttgcggaa aagaatgcaa aactgcttgc ggagtgagga tttaccgagg
     5821 tatagttaag aagagaaagc acagttagag aggagtgctg tgagatggct ttgatctatg
     5881 tagtagagga tgacaaaaat attcaggaga tcgagacctt tgccatggcc aatgtgggct
     5941 atgaggtaaa aggttttgat tccgccatgg atttctatga ggcgctgaat gataccacac
     6001 cggatctgat tttattagac atcatgctgc cggacgagga tggtctgagc attgtgaaaa
     6061 agctgcgtac cagacaggac acggtgctgg tgccggtgat catggtcacg gcgaagacca
     6121 cggagatcga caaggtcaag ggactggatc tgggggctga tgattatatt acaaagccct
     6181 tcggtgtcat ggaactgatt tcccgggtaa aggcaatgct caggagaagt cagaagagtc
     6241 aggaaaaaga taaggtgctg aagctgggga acattgtgct ggaccgagag aaacattctg
     6301 tcacggtagg caaggagcct gtggagttga cctacaaaga atatgagctg ttaaagcttc
     6361 tgatgaccaa tgcgggcatt gtgaccacca gagaactgat cctggaccgc gtctggggga
     6421 tcgattttga gggagaatcc cggacgttgg acatgcatat caagacgctg cggcagaaac
     6481 tgaaggaaga gggcagtctg atcaagaccg tgcgtaatgt gggatacatt atgaacggcg
     6541 agaaagcgta acagaagatg aagaaaaaga ttaacaaaat gatgatcctc attgccacgg
     6601 taacgatttt gcttaccatg gtactgatca cagtcgtgta ttatgatctg ttccgcagac
     6661 aggtgctgga ggatctgaaa agctatggtt ccctgttaaa aagctgttct tcggtagaag
     6721 agatcaagga aaacggagtg ccggtggaat cggatctgcg attgacgctg atcggcagtg
     6781 acggacaggt attgtatgat aatgaggcag attctgtggc catggggaat cacgcttccc
     6841 gtccggagat ccaggaagcc atgcgggacg gcgaaggtga agcagtccgg aattccgaga
     6901 ccttgtccca gtccaccttt tattatgcca tccgtctgga agatggcagc atcctgcgac
     6961 tgtccaagga tgccagaagc atttacagca ttttcagtca ggcgctgccc atgatggtgg
     7021 ggatctttgt ggtgctgctg gtgctgtgca tggtggcggc cagagtgctg accacgaagc
     7081 tggtggctcc cattgagaaa ctggcacaga atatcgggga atgtacagag atgcagacct
     7141 atgaggaact gacaccgttc atgaccatga tccgcaaaca gcatgaggac attatgaaaa
     7201 atgccagaat gcgtcaggaa ttcagtgcaa atgtgtccca tgaactgaag accccgttga
     7261 catccatttc cggctatgcg gagctgatcg agaccggcat ggcttcggag caggatacgg
     7321 tgcgttttgc tcacgggatt cataacagcg caaatcgtct acttacattg atcaatgata
     7381 ttattcgttt gtcagagctg gatggcacgg aaggggaaac ggatacggag cttttgaatc
     7441 tgcatgaaat ggcgcagaat tgtgtggaaa tgctgaaaat gagtgcagag aaacacaagg
     7501 tgaccatagc gctgaacggc acggaatgct atgtgacagc caatcgtcag atgatggaag
     7561 aacttttata taacctgtgt gataacgcga tccggtataa caatccggga ggaagtgtgg
     7621 atgtacagac ctatgccaga gaggggcata cctatctggt ggtaaaagac accggcatcg
     7681 gcatctccaa ggagcatcag gagaggatct tcgagcgctt ctaccgtgtg gacaagagcc
     7741 gctccaaatc caccggcggc acaggactgg gccttgctat cgtaaaacat atcatcgcca
     7801 agagccacgc tgaactggaa ctggaaagtg aacccggaaa aggaacgacg atacgggtga
     7861 ttttctgaga acttcgagtt caaattttct aagacttcat tcgagagagt ggagtctttt
     7921 ttacgcatga tattccgcca gcagctgttt cgcataagca acacagtctc tgtgcagagc
     7981 aaattttttg ccatcgcaca tgatgacttt gcggaagccc agcttctcca tgtatctgcc
     8041 gttaaggatc gtcttgacgg tgggagcgaa gaaggtaggg tgattctcta tctgagagaa
     8101 caaattgata gcggtggtgg ctacttttac ggtgcggccg tccttcaggg tgacgatgac
     8161 attccggcct tcttcttcgg cgtagaggat gtccggttcg gtgacataga cggtatcttc
     8221 atcctctcga agctctatga gaggaactgc cttagacata atatccagag catggtcaat
     8281 actctgtccc agttccgcta ctttgatggg cttgtccaga aaaataacat tttccggata
     8341 ggtctgttcc ctgtaattga tcttggaaca gcacagcacg atgggtgcgt tgaccccctg
     8401 ggcaatcagc gcatttgcca cttctgtacc ggtgatgcct tccgtcagac acatatcaat
     8461 atagaaggca tcgtattgca tgggattgga cagcagtgag gtagaattac cgaaagaatc
     8521 ggtataaatg atacccgtag acgctgctct tttgtcggat tcccgcttta acagacgctc
     8581 cagctgcttt ctgtcggcta cattatcatc aca
//
LOCUS       DAWEJW010000106         8606 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2697_length_8606_cov_38.158812, whole genome shotgun sequence.
ACCESSION   DAWEJW010000106 DAWEJW010000000
VERSION     DAWEJW010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2697_length_8606_cov_38.158812"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2574 a         1547 c         2489 g         1996 t
ORIGIN      
        1 agaaaaaggc tgagcagaag aaaacagaga aaaaggctgc ggagaagaag gctgcaaaga
       61 aagaaaagga agagcagtta aaggagaagc gggcagagag tgatgatacg gaagagacgg
      121 taaccattac cgccaattcc atcgaggagc tgctttccag gatagatacc tacaatttca
      181 acacacggag tgacgaggtg cagacccaga gtgaacgtca ggtgggtcag aacatcgatt
      241 tcagagggta aggtgatatt gtatggtttc tatggggaaa tatggaggaa cgttcgtgca
      301 ctcgaacagc cggggaacga cgcagatcac cttcgggagt aggacgaacc agagcagtgc
      361 aaacggcatt ttcgggcagc agaataacac gactgcttcg aaaaattcgg tcagtgatat
      421 taaacggatg cttgcccggg acagccggag tacgcagacg caggcagcaa acagcctggg
      481 acaggagtct atcgttacca gcacgctgag ctacagtgaa gctctgcgca atcagaggca
      541 gcagacgaaa aacacgacgc tggctctgaa aaaactgaaa tatcagttta aaaatatttc
      601 atccaaaata ctgcggagca agacgtccca ggcggcaaag caggcagccg ggcaggcgcg
      661 gagagaagtc ctgcgcctga aacggcagaa gcagagcggc aattatgatg atgaagagat
      721 cgaagcagcg atcaaccatg ccaaagctat ggagcgtgta gcgaagaaaa aggcaaagca
      781 tctggaagaa gaggaaatgg caaaggcagc cagaggaatc tggcagggtg aaaaagtctg
      841 cacggacgaa gaagagagaa cttcagacac ggatcagaat acggacccgg aagccaaaga
      901 ggaattgccc tgtgaggctt cagatgcaca gagcgatatg gatctggaga aatatgatta
      961 tccgcagctg gatgaattct tgttacagac cagtgagctt atggtggaaa taagcgattt
     1021 tacttccgag atgatgcagg aactgtctga cagtatgcgg gatatgatgg atgagatggg
     1081 aatggatgag ttatccgata ccatgctctc tgtgaagaag gacatcgatc ctgcagacat
     1141 taagatgatg aagattaagc accgcaacaa agagatgaaa gaaatcgtca aggcaaatgc
     1201 ggaatacctc aaggcagtgt ttgaccgtct ggagaaaatg aaggacaatc ccgtcattcc
     1261 ctccggaggc ggcatgagct tatctgccgg atcctccggg acatcttttt ctgccgatgc
     1321 cgctgggatc ggtgtcccga ccgtacctgc atcggtgatc gatatttcgt tataaagtag
     1381 tattctgagg aacttagggt atcccgattt tcatacatta aaagatatga aaatcgggat
     1441 atttttataa aaatatgcca acatttttga tttacgtgaa aattgactta taagggagaa
     1501 aaaggagaga gcggatgaaa acagtagcat tgttgtatga taccagttgt atttacgaaa
     1561 ttgtgatact gaattatttt ctgaaggtaa cgggaaaaga aatgcagttt gtatctctgg
     1621 acggaaaaga aatcaccgcc acggaaggat accggatagt gccggaggat cggctggatt
     1681 ctgcggatcc gaaggatgtg gaactgctgg ttattccggg aggagatatt gagaagatag
     1741 atattccgga ggtatggaag tacctgaaaa gcgtaaaggg cctgggcgga cggatcgctg
     1801 ccatctgtgc aggtgtggat gttgtggaac atgcgggact gttagagggg atcgaatcga
     1861 cgcattccgg agaaaaggat gtggtaactt ccgaccggat taccacttcg agagcaaacg
     1921 ggtatgtgga ttttgcaatc gaagtggcga aacagatgga attgttcgaa gatgaagcgg
     1981 atctgcagga gaccatcgct ttctggcggg aatttcggcg gatagagtaa tcctccttgt
     2041 ttttcgtaac aaaactccag cgccagctcg cattcctgcc tgtgcgcaca gatatattcc
     2101 atgatctgcc gctcgtttgc ctccgcgaaa ctcttttcca ggaattcctc acgcccgcag
     2161 cgtattatcg tgggtcgaga cctttcgggg agtcaggaat atgagtctgt gtcctcaaga
     2221 tcggttttgt ttgacaatac gaaaaaaaca aattacattt aaaataatac taatatatga
     2281 aaggtgaatg caatgacgat tgaagaaatt ttatcaaagg aagaaaatca gacatttgat
     2341 cgaaaaagta taaatatttc tccaaaggat ttagcaatac caattgttgc atttgctaat
     2401 gcggatggtg gagatattgc aataggtata accgatagga caagaagaat tgaaggcatt
     2461 gattttgaaa caaagaagtt aaatgaacta ctgagagtgc catttgattt ctgcaatccg
     2521 actgttaaag tcggaataga aaaacttcca tgtgtcgatg aaaagggaag agagaatcat
     2581 gtgcttgtaa tgcatattga tgcaagtcca caagtacatg caaatcaggc agatgatgta
     2641 ttcttaagag ttggtgacaa atctaaattg cagacttttg aggaacgtct ccaactcaat
     2701 tatgataagg gagaacgtta ctttgaagat aaagccgtac ccgatgctac gatagatgat
     2761 atagatatgg actttgtaaa agaatataca gataagatta attatggaaa aagtccattg
     2821 gaatatctta aagagaacag aggatttata aaagagaaag gtggcgagat acaaatcagt
     2881 tccgctgcaa ttttgttatt tggaaaaaat ccgcaaaatt tcttccctcg cgcaaggatt
     2941 cgttttatac gttatgaggg aaccgaagaa aaatttggaa cagagatgaa tgtaattaag
     3001 gatgttatct ttgaaggaac actgcttaat atgataaatg aggcaattgc atatttggat
     3061 acacaggtga aggaaaaaac atacttggga tcagatggaa catttgttac agatgaggaa
     3121 tatcctaagt ttgtcagaca agaactgatt gtaaatgctg ttactcatcg ggcgtacagt
     3181 attactggta cagatattca gattaaaatg tttgatgatc atatcatagt agaaagtcct
     3241 gggaaacttc cgggacttgt aagagcagac aatattcgtt ttacacattt ttccagaaac
     3301 cctaagattg cagagttttt aaagaactat aaatttgtaa aagaatttgg tgagggtgtt
     3361 aatcgtatgt gtaatgaact ggagcaggtt ggattaaaag atccggttta tcatacaaat
     3421 gctttcatgt tgcaggcggt tatttataat tccagtgctg aaaaagttgg ctatttatct
     3481 ggaaagttgg ctgctgagag tgaaaagctg gctattgaga gtgaaaagtt gtcttttcaa
     3541 aatataaagt tggctattga tagtcaggca tataatgaac caacgaaaaa aaacatatta
     3601 aaaatatatg aagaaattga aactaatcag atatttggag cgcctgagat agagagagta
     3661 ttgaaatgtt caacttcgac agctaagaac gttatgaaaa aattaagaga aataggtgtt
     3721 gttgaagaaa ttaaaggaaa aggcaaagga aagtatactt ttatttctga attcaattat
     3781 actaaaaacg taaattaagg tggaaaaaca tcgcaaggca ctggattcat ccggtgcctt
     3841 atttttaggg aaccttttta tccattcgcg actcatatat agtgaagccg gcgataagaa
     3901 ggaggtggta ccttggcagt catgacaaaa gaagagtttg tgcaacatgt gaagagtgac
     3961 acgcttccca tgtaccgact ggcgtattcg attctgcaaa acagtgcgga tgcggaggac
     4021 gcggtcagtg aggctatcgt gcatgcttat gagaaattag acacattgaa aaaagcagac
     4081 agttttcgta actggatcct gcagattgca gccaacgagg cgaaaaaaat ctataggcag
     4141 aacaagcgca ccagtccggt ggaatgggaa gagaacctgt ctccggaatt ctacgatgag
     4201 catcatgagt tgtgggatgc ggtgatgcag ttggacggcg ggtatcggga tgtaatcgtt
     4261 ctgttttact atgagaggtg cacaatcccg gagatcagca ggatcctgga atgtaggcag
     4321 ggcacagtga aatcccgtct gcatcgggcg aaagagcagc tgcgggcaat gttgtagcag
     4381 cggaaaggag caggagatgc aaagcgattt tttagataga aaattatata aaatggcaga
     4441 acaggaacag atggcggtat cggaagatct ggatgcgagg gtggatgata ttttacagca
     4501 tctggagaaa aacggcagag gcagcagaca ccattacagc ttccggcggg cggcgattct
     4561 ggcggcagct tgtctggtac tggcgtcggc gacggtgacg gcatccggaa tgtatcaggc
     4621 acggatggaa agcatgaatc atgaaaaact ggaggagtat tttgcccagc tgcaggaggc
     4681 aaatgtctct gcggatactt acagcagacc gatgacagag aacgagacgg cgagactgga
     4741 agaacttagg caggcatacc tggagcaggg gtatttcccg aagaaggagc ttacgctgat
     4801 tgacaactcc gaaaaataca aaaagggagt ggctttctat gcggagcgga gtaccttttt
     4861 cctgccggag aaggagctta cggatgagca gctgctggag ctgatcgatt ttaaggaaaa
     4921 gagagaatac agtctggcga agatcagcga ggagattgaa gcaggttctt atgagtatgt
     4981 gaagacagag ccggaaaagg atgctgcgca gcagggacgt gcagcacagc agggaacgag
     5041 tggtacggtt ctcggcgccg atacggcaac gcaggaatgg cagattgctt atgagggaga
     5101 cgtaagtatc cgttgtgcgg cagtggcaga gaacggtctg tatgtgggcg gtttcactac
     5161 ggacggacag gcgagagtgg aatatctgtc ttacggcagc ggaacgccag agacgttttt
     5221 tgaagatttt gcagaaaacg gagaggtcta cagtctgtgc atcgcaccgg acggaagtgt
     5281 ctatgcggca ttacgcggac tggcatctcc gggagcggaa catggccggc tgcctgtgat
     5341 ctaccatatc agtccggagg gaaagcttct gaacagtttt accgtaggag agcgggaatg
     5401 gaatatcgtt gatagtatgg caacagatgc ccagggcaga ttatatatgc ggatgcggat
     5461 gcaggaggac ggcagttatg tgcggatcta ttctgcggaa ggtgagctga tcgggaccgt
     5521 ggcggatgac gacagctatg ccgtacaagg tgccggagca ttaggacagg gcaaggacgg
     5581 caaagtctat gcggctgcac aggcagtggg caatacgaca gcaggagata tggctatgac
     5641 attagtgtca gtagacctgg aaagcctcag gtttgttccg gtggctggag gtgagaatgt
     5701 tatttgcagt gctgatacga tctacccgtg gaaaatggtg gcagcaggtg tgagtgagac
     5761 ggcagatttt atcctatggg gatacaacgg cgtggaaacc tatcgtctgg gtgagacgga
     5821 attcaccaga gtgcaggaga actgggagat gccctgcggt acggaaggta cctgtgcggt
     5881 cctcgtaccg gacggtcgca ttctctatat cggcgcatcc gacggcttcc agggaacgac
     5941 ggcggacggc gtggagatca gcggaaaaga tcctgagaag acagtgctct attatgagcc
     6001 acttgtaaaa tagagtgaca ataaactggc gacaccggga aaacgatgcc gccagtttta
     6061 tagttccaga ctgttaagtt tccatctgct caattgtgca tttacagtgg ttatattata
     6121 aaaagaccag aaataacaga aagcggtcgc ttgggtgaaa aaagtagatt caggggtgaa
     6181 attttgttat actgtttgta acaaacggag tataaaagtc gtcaatgtag atagaaggga
     6241 aacaaaggag gggtttatga agaagattaa tgtaaaagca accatttttc tcagtgcaat
     6301 cgtattggtg gtagtggtac ttttgctgaa tcttatcgaa ttccagaaag cagtggattt
     6361 cacggtgccg gggattcaga tgccgaagga gaatgcctat gggaagctgg aagaggtaca
     6421 ggtgagcgtt aatggaactt attattaccg agtactgccc tggatccacg gcagcacttt
     6481 ccatgggcag atatctttcg ttggagaggg agaggaaccg cagaattatt tctttaccta
     6541 tggagcaagc agaggatttc tgaaggaggg aagaggggct ggctggatcg gaaccccgga
     6601 tgctcagact accgtagcga tttatcaggc ggtacagaat gtggaaaaag ggatgatatc
     6661 cgtagagaat ctcgccagct acgttttata tccggcggca acaccggaag aagcctatgc
     6721 catagcggat atatggtatc cgggaatgct ggagaatgaa gcaaagacag cattctgcgc
     6781 ggcttttttg ccggaggaat gcccgcagaa gattggatgc agcttctgtg cggaggatta
     6841 cgattatgtg gaagatgtcg ataccgtaga gcagctgtgg caggaattta tacaactttc
     6901 cggaaaagtg acgtcagatc cgatcccgcc ggaagagatg gtggaaggtg tgggatattt
     6961 tcagtttgtc tatgcagacg gtgagacaag gacctttcgc tacgcgtcgg aagaacaggg
     7021 attatggata ggtgaggacg aacgctgcct ggtggacggc caggctttct atgactggca
     7081 ggagcgtctg atggaagcgg tgcagaagta agcacgggaa agagaggggc ggaaatcttc
     7141 tccattcgca gcagatccca catcggacag gggtatgcga agcagtatgg tgaggaactg
     7201 cggcagtatt gggaagcaac caatgaggtt tttgtgtgga tcagtgaaca gtatcggaaa
     7261 ccgttatatg tcacttcgga tgtgaccatt ggagatggaa agacagtgat ctcgttccgg
     7321 gggaccggca cttccatgac taccggggag acagaggaaa tccatagaga ctgcgtgctg
     7381 gattttgaaa tgcatgcgga ggtattgcgc tcggaagaag agccggagct gacagtaagt
     7441 gactggaatc cggaagaaga gggacaacag aatcagattt cttacttgac agataggtcg
     7501 gtagaatgta gactataatt acaaagagtc tataaaacat cgactatgcc ttgccggggg
     7561 gcaggacagg aggggcttat gggtgagaat atctacagaa tatttgacgg tgtatggaac
     7621 ttaagtctgg tgggaggata ctgcgtgctg ctggtgatcc tcgccagact tttgttgcaa
     7681 aaagcaccaa aatggtgctc ctatctgtta tggggaatcg tgtttgtgcg gttgtgctgt
     7741 ccgatactac cggagacagg aatcagtctg attccggaca aattgctgtc tgtggggaca
     7801 gagatgacgt ccgggcagca gacagtcggt ccgggcagtc atggttatgc agaggccaac
     7861 gatacgaagg acatcggaga tgtcctgcgg cgggagtacg atgcagagga agtaccggac
     7921 agccagacgg cagaaaaaac gatggttgtg ggaaatgaca atactgtcat gaatgctgac
     7981 ggcacgcagg atttttccct gggaactgcg agaacagaaa gtggagctac ggttgcagag
     8041 gtagcagcca gtggaacaga tgcagtgatt tcggaaacat ctgccggaac gggtactgac
     8101 gcctgcgcat ggacataccc ggtgtggttc cgtgtattat ccatggtatg gttggcagga
     8161 atgcttggct tcctgggata tcatgcttat tcttacctgc gtatgcgtaa ccggattcac
     8221 cgacctgaca gcggtgtcag acaggtggag ccggggatct gtgagatcga tggagggcat
     8281 ctgtccttcg tcatggggct gatccatccg gtaatctatc tgtcttcggg cttagatcct
     8341 gagagcagaa aagttgttct gtgtcatgaa cgggtgcact tacagcgcag ggactatctg
     8401 tttaagcctg cggcgttggt gatctgctgc gtccactggt tcaatccgct ggtgtggctg
     8461 gcattttatc tgatgaatat ggactgtgag atgtcctgcg atgaaaaagt tgtgaagctt
     8521 ctcggcgagg agagcaagaa gatatattcc gatacattgc tggaagaggc ttccggtggg
     8581 gaatggaaac gctatcgggg cggttc
//
LOCUS       DAWEJW010000107         8512 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2730_length_8512_cov_58.369635, whole genome shotgun sequence.
ACCESSION   DAWEJW010000107 DAWEJW010000000
VERSION     DAWEJW010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8512)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2730_length_8512_cov_58.369635"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2800..2899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2036 a         2369 c         1723 g         2284 t
ORIGIN      
        1 tcgctgtctt cactatgcct atgcgcggct cattgctcgc gtaactttcc atttagttac
       61 cgcactctac gctgatgcgg ttgaagatcg ccatgatctc ctcggtgttg atggtcttct
      121 tctcttctcc ggctttgtcg aggatggctt ttccttcgtg catcatgatc aggcggttac
      181 cgtattccac ggcatagcgc agattgtggg tgaccataat agtggtcact ttcttctcct
      241 ttacgacctg atcggtgagc tccatgatga tctccgcagt cttgggatcc agcgcagcgg
      301 tatgctcatc cagaatgagg aaatcaatcg gtgtcatggt agccatgagc agcgccagtg
      361 cctgtctctg tcctccggag agggagccga cctgcacacc catcttatct tccagtccca
      421 gatttaaggg acgtaacatc tcgcgatact cttccagctt tgctttattc acaccacgtc
      481 ccaggttata aggcttgccc ttattctccg caatggacat gttctctaaa atcgtcatgc
      541 cggggcaggt tcccttggaa ggatcctgat agactctgcc gatccgacgc tgacgaatat
      601 aatccttctg tctcgtaata tcttctccgt ggatcaggat cttgccgctg tccaccgcaa
      661 tgcttccaca gatgatgttc agcatggaag tcttaccgga accgttgctt cctacaacgg
      721 atacgaattc tccgtccttt accgtaaggt tgaaatccct gaacaggcac agctcgttga
      781 ccgaaccggg attgtaattc ttgttgatat tgataagttc tagcatgctt tcaccttctt
      841 tctgtccatg ctgaccacca gaatgatcag gaacaggact gctgtgatca gcttgatggc
      901 attagggctg atacctgcga acatggctac tgccacacat gccttataca gaatggatcc
      961 caccacaaca ctgctggtca cacggaaaaa tgttaccttc ttgaacaggc tggtgccgat
     1021 gataacgctg gccagaccga tgactaccgc accggttccg atggaaatct caaaacttct
     1081 ctgctgttgc gcatagatac aaccgcttaa ggataccaga ccattggcaa tggacagccc
     1141 cacaatcttc accagtcctt tatccacacc gaggctggta acgatagtat cattgtcacc
     1201 tacggcacgc aacaggaatc cggacttggt actcatgtac cagtctaaaa gatattttgc
     1261 caccatggtg atcaggaaaa taatgatcac atttttgaaa ttcgacaaaa aagagggggt
     1321 aatgctgttc acaaagctgt tgttgaaaat attttcctta tcaaaaatag ctacatagcc
     1381 ggagcctgcc acccacagat tgatggtata cagaccggtc atcataatga taccggaaag
     1441 caggtctctt actttcaact tcacatggat caatccggta cagattccta ccagtgctcc
     1501 gcctaagaat gcaatgggaa gggtgaggat tgccgggacg ccgaaaccgt tgatcagacg
     1561 cacagtgatg gcagccccaa aggggaagct gccatcgacc gtaagatccg ggaaatccag
     1621 tattttatag gtaatgtaga cacccagtgc caggatgccg tagatcattc cttgttccag
     1681 tacgcttaat aataacgcca tatttttctc cattcttaaa atcctgtata tccggattac
     1741 tctattattt tctgcctgtc ggcagctatt tactgattac tgtactacga tctcatcaaa
     1801 ggactggtaa gcatcggtct tgtaattctc gggaagttcc aggttgatct tctctgctgc
     1861 tgcaaagttc acatacaggc tgggctcgga gattacttcg aaattcagtt cggaagcctt
     1921 tgcctctccc ttcaggactc ttgctgccat cttaccggtc tgcttgccga gttcgatgta
     1981 ttccagaccc atggatgcca cgcagccagc cttaacctgc tctacttcgg aaccaaatac
     2041 ggggatgcct gccttgtcag cctcatccaa taccgtctgc agtgcgctta ctacggtgtt
     2101 gtcggtcagg ttggtaatgc agtctacctt ggatgcgata tccgctgctg ccagagctac
     2161 ctcagacatt gcggtaacac cgctgtccac gatctcgaat ccgtagttgc ctgccagagc
     2221 cttgtactct gcgatggtac tgatggagtt tgcttcgctg gtggtgtaga tgataccgat
     2281 ggtcttcgct tcaggaagta cctcacggat cattttcaac tgtgcatcta ctgccagtgc
     2341 atcggaagta ccggtgatat tgcctacggg agtaccatcc tcattggcca gacctgcttc
     2401 cacaggatca gatactgcag tgtagatcac gggaatatct gtattcattg tagcattgta
     2461 tgctgccata gcgcaaggag ttgcgatagc acagatcata tctactttct tacttacgaa
     2521 gttgctggcg gtcatggcgg aagttccgtt gtctgcctgt gcattgacat attcgattgt
     2581 caggttctca ccctctacaa taccttcctc tgccagcccc tgaataaatc cttcacgaca
     2641 gttgtccagg gaaccatgta cggcgtactg catgatacca atggtgtaat tgtcctttcc
     2701 tgcggtgctg ccacatccgg tgagggcacc cattgccata actgcggtta atactgctgc
     2761 caaaattctc ttcttcataa tcttattcct cgctttctgn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnc tcaaatgtat attgctatac acttgagacg gtaataagct
     2941 cttgctcatc atcgtggtgc cactcaattt gacaattcct gcgaattgtg tccactctct
     3001 ctggatacta tcatatccat cggcttgata acgggtccga atcccgctga cgcctaaaac
     3061 tgatctgttc tcgggccagc ctcggaagtc cattcggcga aagttccttt accgtgatcc
     3121 caccatccac ggctctctgt aaaattccct aaggcttact actcttcctc acaggtttgg
     3181 taaacttatg aatgttgttt atgtgtctta gaataatctc tattttctat tttgtcaata
     3241 ctatttttag aaaatttaca aatcttatcc gtactgtcac atacttacat tcgtaaaccc
     3301 aacgaatcca acgaacaact gttactctgt ctctggcaga gtgatcacca ccttgaacag
     3361 gtcgccgtcc gtgacaatct gcatatcacc attctgcagt tccgtcaggc tcttggccat
     3421 ggataatccc agaccgttgc cctccatgtg gcgggatttg tctcctctgg tgaaccgctc
     3481 ttccagttcc tccggtgaca tctccagcgg gaaggctgac atattccgga aaatgattct
     3541 cacatcctga ccgtcatgct ccacgttcag gtagacgcgg ctgcctgcct gggcatattt
     3601 gtaaatatta ttcagcagat tgtcaaacac tctccacaga tgcctgccgt ccgccatgac
     3661 ctttacggtc tcctccggct gttttacgat agtttccagc ttcttgtcag agaatcgctg
     3721 ctcgtattcc cccgccgcct gtgacaacag taccgataca tcgcagactt ccgggtgtac
     3781 ttccagattg cctgtggtgg ctttggaagc ctccagcaga tcatccaaca gttttttcaa
     3841 cttcttggac tggcgtaaca atacttccgc atactccgac agacgctgtt ccttttcgga
     3901 atccgtctcc gccgtcagac cattctctgt tgctgcattc tcttccactt ccttcccgat
     3961 gagatccgca taattaataa tggaagtcag cggggtcttg atgtcatggg acacattcgt
     4021 gatcagctcc gtcttcagat gttcactttt catcctctgt tccacggctg cggaaattcc
     4081 ctcgccgatg cggttcagat tctctccgtg ttccttaaag gacagtccca tatgagaggt
     4141 atccagctta taattcagat ctccgtccgc caatgcttca ctgccctcct gcagttcttt
     4201 gcacatcaat gctacaaaag taatcgcacc gcacagaata aatttctcta tggcccagaa
     4261 aaagagtaat acatctgtat cattataaaa cagcattaaa ccgaaaaact ctaccacgca
     4321 gactgcaaga cacagaagta ctattctcca taccatagga atcccacgga tcaatttcac
     4381 aataccacgg aaaaacccct tgcagaatct tccgaagaac cggaacactc tatacaggat
     4441 cgtattctgc caccattttc ccattttcag gcggatggcc agctccatgc agtacagggt
     4501 gcaccatatc acctctgcgg caaacagtac caccagcacg atcagattga tcatacctga
     4561 gttcataccg atccatccaa aagccaggta cagaccggta aaaactccaa tagcaacgac
     4621 cactgtatac acatccaggt ggatctcatg gatcgcactg ggggtaatac cctccctgcc
     4681 attcttatgt ccggcactgc acaacaggaa gataaagcat aataatgcca gtatcgttcc
     4741 cgtacagcat atcacaggga tcacatttcg gttatcataa aactgctctg cctgatgata
     4801 gatcagggca tattcgtctt ccttcggcag gctgggattc actgtcaggg taaccacaat
     4861 acgcttgtta cgctctgcta atgcttcctc tgcagcctct tcctgctgcc gttctgtctc
     4921 acttaaggca tccttgatca tccattttgc atattgttcc gcccgttcaa ccggcacata
     4981 ggtctctcca cctaaggata cgctgtcccc cagatccaga tatttctcca gattaccaga
     5041 ggaaagggct tcctgctccg ctgccgttat actttccaca tcagacccgg tatcgacaga
     5101 tggcgcaagt tccatgatat cagagctttc agagcttcca taactttcat agccatcata
     5161 ggcagaggaa gtttctgtgt tgatataggt gttatcattc ctatgctgca cctgggactc
     5221 ccgccggtaa gtcagatcat ttgtcttgtc tacataatcc tcgttcttcc attctcccag
     5281 gaattcatcc gtatcctgat cgtagctctc cacctttacc cagatgttcc gctgcctgca
     5341 ataagcttcc atggtctgca agctctcatt ctcataccag tcgcgtatcc gatacagatc
     5401 ctgattgacc tgccgataca acagctgatc atggataaac atcattttac cggaggaata
     5461 cgctccacta tctgcaagca tgcaggtacc atagagtccc aacaccccca caattgcggc
     5521 tgcgatcatc agaacaaatg ccacgacctt tgccaatgtg ctgcccaaca gactgtttcc
     5581 ctgtgccggc gcattctttt ttttctcggc tttttctctt tttttccgtt cccttttatt
     5641 ctctgacaaa gctgtttcta ccatcgtact cactgtattt tccaccggat tatccactct
     5701 gtcctgttct gtattcctgc tgttttcttc cctgttatcc atattcctgt ctccttactt
     5761 cccagagcag ctccgcgctc ctgcctgctc ttatccctgc ttctcacact tgtagccctg
     5821 tccccagacc accttcagat atctgggttc tgccggattg atttccaact tttcccgtaa
     5881 atgccggata tgtaccgcca cggtattctc gctgcccagg ggcaggtcgt tccagattct
     5941 ggtatagatc tccttcgggg aaaatacctg tcccggattc tggatcagaa gctttaagat
     6001 ctcatactcc gtgggcgtca gcgtcaccgg ctcgccgtcc agcatcaccc ttttctcctt
     6061 gtcatccagt tcgataccac cgactcttat cacatctggc cggacagtac ctccacccag
     6121 ctgcatatag cgacgcagtt gggatttgac tctggcggat acttcgatgg ggttaaaagg
     6181 cttcgtgata taatcgtccg ctcccacggt aagtcccagc accttgtcgg aatcctcgcc
     6241 cttggcagtc agcaggatca ccggcacgtt actcctttcc cgaatcttga tcatagcctg
     6301 aatgccgtcc atcttcggca tcataatgtc catcagcacc agatgtacct ctttcgtctc
     6361 caccacatgc acggcttctt caccgtcaaa agcctccaga aaatgataat tggtgtcatt
     6421 taagtatatc tttaacgcgt ttacgatatc cttctcatca tcacagatca atatattgta
     6481 catactctcc ctcatttcca taccggccat tgccgcccgg caccttttcc gcaagggctt
     6541 cccttacgct aaaatcattg taacagaatc cagattaata aaaaacagga aaaaccttta
     6601 agatttgttt aagacgtctt tacataaaac accctctgcc atgcccagaa gggcaaagac
     6661 aaagggtgtg gataatacat gttgaaaagt gaacagtgag cagatcaaat agccggtgag
     6721 gccgaagaaa actgcaagtg cccggggatc cctggttgcg gagattcgaa ggttccggaa
     6781 tgcggtgagg aagattccac cgtaggcaat gactcccaga attccctgct gtaccaggag
     6841 ggtcagccat tcgttgtggg cattggaata gatattgttg tccagaagtc cccggttgat
     6901 ccagtccatg gcgtcactgc cccattgata gagcgcataa tagaagctgt caggacccat
     6961 accgaatatc tgaaacagga gtccctcttt tccgtaagta agaatcgtga ccttccacag
     7021 tgccagtcgg ccgttggcaa cttccgacac tttatcactg ccgggcagat atcccgccac
     7081 cagcagacag ccaagaagca gtgccgccag cgtcaccgta accgcaagcc ccacaacggt
     7141 ccgaaaaaac cggccactct ccagccggtc acttctctcc tgtctctccc gcagttgcaa
     7201 aatcagatat accgccactt ccacaacaaa caccacatac caggccgggg taaaaaagag
     7261 tcttttttcg gcatcccctg ccagcaccag gccggtcaga tgtaacagca aaaacatcct
     7321 gaccataaat accgaaagcg gtaaaagcat caggatctcc aacgcccgca gcagcaggcc
     7381 tctcttccgc agagaaagca acagtaacag caggcttaat ccgcaggctg ccagtatagc
     7441 tgtagtactg ttggaagtaa tagccgatgc aaaaaacaaa aaactcccga gcagtcccag
     7501 tgctctgacc tgttgttttc ccataaaagc tgctgccaga gagatacctg ccgtcacact
     7561 gatatagcct gcataccagt tattgttacc gatggtggac aacagctggg tccggttcca
     7621 ttctccgttt tccattccgt ggaaagttcc cagcacatcg atgtccagac ggttcaacag
     7681 tccgatcagt gtcacgattc ccgctgtgat cccggcgatc caccagatac ttctgctgcc
     7741 gtcataatac cgggataccg caaaatagat ccccaccagc aatccctggg tcaccagccc
     7801 catataccag ccgggatacc ctaaaaggac atcctccctg cagggactga ttccataaga
     7861 aaagattgca gacaccagat acaacagcat aaacacatcc gttttcttcc acatccgttt
     7921 cgttgtccgt tctccccgga tcctctgcca cagcacagcc agcaccacaa aagcccccag
     7981 caccagcgta gtattccgga aaaacaaata ttttgcattt ccgatcatca ccagcttgtt
     8041 ctgcatatac agcggtaaac cggcaaaaag aatcaccaga tacccataaa ccagcatact
     8101 cacggtctct tttgtatttt gttcctgttt cttcatagct tacattatcc ttcagactga
     8161 aaaacaaggc acccctcatt gactgaagag tgccttatat ttcccggtta ttttatttcc
     8221 ttacggttac ttatttgccg taacgcatct gcaggtacag aggaccatac cattcgatag
     8281 cattgtcttc tgccttaccg gcagggatgg ttaccgtata ctcgttgcct tcgaaggtgt
     8341 actccaactc cagagctacc gtctgcttct tgtacagagc ctgcatgata tcgttgggca
     8401 gagcattgat attcttgtct ctgctgatct taacggtagc accgtccttg gcattagcga
     8461 tctcgcttgc cacattgtca cgatatcttc caaatgcgtt gtcagtacct tc
//
LOCUS       DAWEJW010000108         8403 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2775_length_8403_cov_48.954720, whole genome shotgun sequence.
ACCESSION   DAWEJW010000108 DAWEJW010000000
VERSION     DAWEJW010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_8403_cov_48.954720"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2284 a         1932 c         1383 g         2804 t
ORIGIN      
        1 tatatccatt taactatagg aacatatgca caattgttag tataccgaaa atattgttat
       61 tttgcaacct tattcctcgg gaaaattaat atacccctgt aaccagatga cacccttgaa
      121 acggcgtccc acctgaggtt ctccgtagat atctttttca ttgatacata catcaaaaga
      181 taattcattg cacagaatcg taagaatata taccttttct cccgtgacat cgttggttgc
      241 cagacgaagt tccgtaatct cacctagtac agagtactgg tcgcactcca cgccgtaggg
      301 cataaaataa gtatccacca gactgaaaat atcatccttc tggatcctgc gggaaatggt
      361 ggtgtacata tccatatctt ccagcgtcag ggtctcaata gcatcttcgt ctccctgtct
      421 ggcagctgcc agaagtttgt tacgattggc ggagtctttt tttacccgga ggacctgttc
      481 ttcatctttt ttgatgggta acaggatact tccggtcagg gacaggccgg ataaggtcag
      541 tgtggtaccg cggatgggta attttccggt actctgagcc ttgatgtagg gaatccggtt
      601 ccgcagatag aaaattagag agattcccac tttgatatcg tcgcagatac cggcatagga
      661 atccttctcc gcatgacgtt ccacagagac atcttcataa gaagtaatgc cgcttcccgt
      721 cagatacgga taataatatt catagataaa cttatcgtta tcatcaaatt ctccacagac
      781 agccacacca aaggtaccgg attctatggc tcctctggtc gcggtgtgat ttttgctgaa
      841 ttcacccaga aggataccct cctgattcat ggtatatgcg cgatgatcgg aattcatgat
      901 cacgtctgtc agtaattctt ttaatttctt acggtccgta tattcactga acccgatagc
      961 tctcatatat ttatgcaaaa catcaccctc tttctgtctg cggtttcatt cactcccttc
     1021 atacaaagta tatctttcct gcaagcagtc atatatactt tgcatggttg agagttattc
     1081 tatcatttct taggcaggac tttctccttg ccacccatgt aaggacgcag agcaacagga
     1141 atgttcacgc tgccatcctc atttacgttg ttctccagga atgcgatcag tcctctggga
     1201 gaagccagta cggtattgtt cagtgtgtgt accagataag ttccgttttc acccttggta
     1261 cggataccca gacgtcttgc ctgtgcatca cccagggtag aacaggaacc aacctcaaag
     1321 tacttcttct gtctggggct ccatgcttct acgtcgcagg attttacctt cagatctgcc
     1381 agatcaccac tacagcactc cagagtacga acgggtacat ccaggctgcg gaagaattct
     1441 acggtgtagg accacatctt gttgtaccag tccatggaat cctcgggctt acacagtact
     1501 accatttcct gcttttcgaa ctggtgtacg cggtacacac ctctctcctc gattccgtga
     1561 gaacctactt ccttacggaa gcaaggggaa taagaagtca tggtaatagg caggctcttc
     1621 tcatctacga tctgtccttt gaacttacca atcatggaat gctcggaagt accgatcagg
     1681 tacagatcct caccttcgat cttatacatc atagcttcca tttctgcgaa gctcataaca
     1741 ccgtttacta cgctgctgcg gatcatgaaa ggagggatac aataagtgaa tcccttgtcg
     1801 atcatgaagt ctcttgcgta gctgatcatt gcggaatgga ttcttgcagc atcgcccatc
     1861 aggtaataga aaccgttacc actggtacgt cctgcgctct ccttgtccag accgttcaga
     1921 cgatccagaa tatctgcatg ataaggaacc tcaaaatcag gaactatggg ctcaccaaac
     1981 ttctcgatct ctacgttctg gctgtcatcc ttaccgatag gaacggactc atcaataatg
     2041 ttggggatga ccatcatgat cttggtgatc ttctcctgca gttcggtctc ggaagcttcg
     2101 agttcagcca ggcgctttgc accggcagct acttctgcct ttttggcttc tgcctcttcc
     2161 ttcttgccct gacccatcag tgcaccgatc tctttggaga tacggtttct ggaagcacgc
     2221 agatcatctg cctcctgctt tgcctttctg ctctcggtat ccagtgcaat cacttcatct
     2281 accagaggga gcttttcatc ctggtatttt tttcggatgt tctccttgac tacctcggga
     2341 ttttctctta aaaatttaat gtctatcatg acttcctcca ttctgctgac atatatgtat
     2401 gcagcattat tctaatcctt tttcaatata tacattcaga acttttacaa gatccacgat
     2461 tctctcttcc tgtaatttcc gtccatcctt atcgcggatg atccggatta tgggacgatc
     2521 ctggaaatct ttattctgag ataccacgat ggcttcttct ccgtcgttag tcagtacatg
     2581 acttccggcg ggatagactg cagtaaaact taaaaacgcg tctactattt taccattata
     2641 tttcacattt ttaaagcttt ttaaatattc tactgcttca tagactctgc tacgtctctg
     2701 ggcaatacca cagatcattt catcaaaggc atcacatacg gctacaatct gacagaaaag
     2761 tggaatctcc gcttctttga gtcgaagcgg aaatccggat ccgtccagtc tctcatggtg
     2821 atacaggatg atttttttta cttcatctga aatccattcc tcatctttta cagaagtata
     2881 tccatatacc ggatgtttct tatactctgc caatgcttcc ggcttcatat cttccatgga
     2941 tttcccttca taatccaaag tcgtatatcg aagtccgata tcatgcagca ggcatcctac
     3001 accgatatca tgaattacct gcttatccac attcatccga agtgccgtca ggatagcgag
     3061 agaactcaga ctgatggaat gttcgtaaat atcagcactt cgttccttaa tatcaaatac
     3121 tttttctaca acttcttttt catccagaat attggtaatg atctcatccg ctgcctgggt
     3181 cagcccctgc aattctttgt tattctggta agtatgtctc tccagaatac tttttacttt
     3241 acttcttatt gttttttcta tatcgttttt aagtattact atttcgttgg cctcgtcttc
     3301 ttttacgtag acctccagaa tacccatttc ctcgatttta tcgatatact ctttttttaa
     3361 tactgcgcct tccggaagaa tgatctgata atcccaagtc atcaatgact gtgcaagaat
     3421 ttcgtttccg gtcagctcgc tcagtttcat tctcttcata tataaacccc tttacttatt
     3481 ctcccattgc catggcaagt gcttctgctt cttctgcccc cagatcaata gcacactggc
     3541 caagtttgat ttcttttgta taagaataac agccttcgga actgtgtaca gaattgataa
     3601 taaaaccatt attattctca tccagcagag ccagtgaaaa actcagctgc ccacccatct
     3661 ggctgaatgc atcgtacttc accaatccca ttttctgata agcggattcc atattcttat
     3721 ataaagtgcg gatatctttc ttatttttct ctgtatttgc ttttaggaat ttattgtctt
     3781 catacagacc agcgatatcc ttttccagac ttgctccatc tttaccgctg aggaatttgt
     3841 ccagtctctt ttttaatttg cttgtttttc tgatctgaac gatcaacagg atcaaaaaga
     3901 tgatcaacag taaaatcact gcacccattc cgatcagaag gtagcccagg tcaaattccc
     3961 ccagaccgat ctgattcaaa aaagtactac tcatattatt aaccgtgcct ttctttcgtg
     4021 catcttcttt tctcttattt attcagtaca tccaacagtc tgtccagatc ttcatgattg
     4081 taaaattcaa tttcaatctt accggatcct tctcccctgg atgtcacagt aacttttctg
     4141 ccaagtttct gcttcaattt ttcttcaata tcctgataga ttacctgcat ggtcttgtca
     4201 tctgtcttct tgggctttgc cggtttatgt agattcttta ccaatttttc cacatcacgg
     4261 acactcagct tttcatcaaa aatacgctgg gccagattgt actgctcttc cggattctct
     4321 acagccagca aagctcttgc atgtccggtg ctgatcattt catctaccac catacgctgt
     4381 acatcttcat tcagtttcaa cagacgaatg gagttggtta ctgcagcacg gctcttggat
     4441 actctctctg ccacttcgtc ctgtttcagg tgaaactctg ttaaaagtct cttgtatgcc
     4501 tgtgcttctt caataggatt cagatcttca cgctgaatat tctcaatcag ggaaatctct
     4561 acaatttcct gttccgtata attacgaatg ataacaggaa cttccttcag acctgccaac
     4621 ttggctgcac gccatcttct ctcaccggca ataatctcat aataatcctt acgatcctgt
     4681 accagaatcg gctgcaacag accaaactgt ttgatagaat ccgccagttc ctgtaatgca
     4741 tcctcatcga aattcttacg aggctgttct ctgtttggct ctacctttgt aattttcaca
     4801 agggtctgcg gttccttttc ttccgttgtt gcttcgcttt ttgctttggc tactgcttct
     4861 ttctttgtct ttgtttcccc taatgcattg ggtatcaggg aatccagacc ttttcccaat
     4921 cctcttgctg ccataccgtt ttctcctata ctcttctctt ctatttattc tttataactt
     4981 cttctgccaa ctgcatgtat gcttctgctc cggctgattt aggatcatac atattgatag
     5041 gcataccata actgggtgct tccgccagtc tgatatttct gggaatcaat gtattataca
     5101 ccttttgatt caaattttgt ttaacatttt ctaccacctg catagacaga ttggtacggg
     5161 aatcatacat agtgaaaaca acaccgtcaa tattgagatt gggattcaat ctttctttta
     5221 ccagattgat agtatggatc aactgactca atccttccaa agcataatac tcgcactgaa
     5281 tgggaaccaa cacactatcc gctgtagtca tggaattgat ggtcagcata ttcaaagaag
     5341 gaggacaatc aataataata aaatcataat catccttaac gtaatccact tcgttcttta
     5401 atatgaattc cttcttatct acaccaatca gttcgatctc ggccgctgca agatttacat
     5461 tggaaggaac tacggataca tttttaataa catttgagat aatacaatct ctgatggaac
     5521 actctcccag aattaactca taaatggtat cttccagttc atttttttca attccaaatc
     5581 cactggtagt atttccctga ggatctgtgt cgattaccaa tactttcttt ccttttgccg
     5641 ccagagatgc agacaaatta atggacgtag ttgttttacc tacgccaccc ttctggttcg
     5701 ctattgcaat aattctaccc atacttattt ttcaccatgt ccttcttaat gatttagccg
     5761 tgcaaacctc tggactgtct gtccatatcc tctacaacga tatcatagat ttcaccgaga
     5821 gaagcacaat actcaagaac cttccagggt tcattggtat gaaaatgaat tttgatcagt
     5881 tcttcatctc ctactgccag cagacaatca cctttgaaat gttctgtgat atagtcgttg
     5941 atctcatctt catccagatc tgttccttca attagtaatt gtgtatcata tttgaattct
     6001 aacattataa aatcctccat tctgtgatac aaaccactct ataataatag catgagcatt
     6061 tctatgttac aaggaaaaaa tgctacaaaa agttgatact ttatactcat ttgttctgtt
     6121 ttttactaga tgttgtattc ttatatctat gttttttcca gtaagcatca tgtttcacac
     6181 caaatatgta aaatgtttca catttttacc cactatatct tgggttgtaa tgtttcacgt
     6241 gaaacattat cttgtataat tttcttgtac atggatagaa tgtttcacgt gaaacattct
     6301 atccataaat atctatatat agtgatcaaa taaaagatat ttgtgtatct tgttttgtta
     6361 caattcctat gataggataa ctaaaaagaa tgtgaggtac gcaagctatg gctattgata
     6421 aagtaaaaga atattttaaa ccccttcata tggaagatca cattttagaa tttgatgtat
     6481 ccagtgctac tgtagaacta gctgcagaag ctctccactg tgaaggaaaa aagattgcaa
     6541 aaacaatgtc tttcttggtt ggagaacaac ctattctgat tgtaacagcc ggtgatacta
     6601 aaattgataa tgcaaaatac aaacacttct ttggtgcaaa agctaaaatg ataccaggag
     6661 aggaagtaga agcaatcatc ggacatgcca tcggtggtgt atgtcctttt gcagtgaatg
     6721 ataatgtgaa aatatactta gacgaatctt tgcaaagatt tgaaactgta tttccagctg
     6781 caggaagtcc taacagtgca attgaattaa caatccctga attagaaaaa tactcaaact
     6841 tcctgcagtg ggtagatgtc tgcaaaggtt ggcaggaacc taatacaatt taatcagatt
     6901 atttttgata gcaaacacag ctgcctgtgt tcgatcagat acatcaattt ttttaaaaat
     6961 attagaaata tgatttttta ctgttctctc actgatattc agagatgttg caatctcctt
     7021 attgaacatt ccattagcaa cttcaataag tacctccaac tctctattcg tcagactgtt
     7081 gatttttcct ttgtcaatat ctctggatac cagtcggctg ttcaatgccg gtaccagact
     7141 gggttgaata taagtttctc cctgcataat acaattaatg gcttttttca gttctgcgga
     7201 ttctgcatct ttcatgatat aaccatctat accaatgtcc acagccttca aaagatattc
     7261 aacttcatta tgtaccgtta ataccaatac tttaacagag atgtttttct tcttaatctc
     7321 ctgcagcact tctattccgt tcataacagg catgttaata tccaaaagca gtatatcagg
     7381 atgaacagaa agtaactgct ccatacattc tacaccatta ttagcttcgg caatcacaga
     7441 gattgtaccg tcaaattcca gtaattgacg gattccttct ctcataagaa catgatcatc
     7501 tgctaacata acttttatac tcatatatat cctcacttgc ttgtgtatgc ttatactttg
     7561 tcctagttgt tcttttttac agctgaatgt aaatctaagg gaattttaat atctatcgta
     7621 gttccccgac caatcgcaga actgacatga attgtaccgt tcagcaaatt gacaccctca
     7681 tggatcaatg ctaatccaaa atgactgttt gctttatctt ccgcttcttc cataataaat
     7741 cccttaccat tatcttgtat taaaatttca tatttctgct ccgttttttt acaatgaaat
     7801 aataccctgg tagcctgtga atgttttttt atatttaaca gacattcctg aaccacacgg
     7861 tataaggtaa tgcaaaaata atcatctgtt tcacatgaaa catcatcaat ttctacatcc
     7921 attatgtaat ctttgtcagc attcaaaaaa tccagtaatc tctcaaaaga agcctttaat
     7981 cccagatcat caaatgtcat aggacgcaga ttgaagatag tacttctgat atcttcaatg
     8041 acattatgca atcctttact cacagaattc aattccatct ttgctctggc aggatcctta
     8101 tcaatatata aggaagccag ttcaatcata tgaacaagat gggcaatatt ctgcaatgaa
     8161 gtatcatgaa gttcggaaac aattcttttt ctctccgtct ccaacatctc catcattttc
     8221 ttaccatttt ctgtattgca actacactca ttcttttgta cattttcatc aatgttcata
     8281 tatcacctta tagaacatat gttcttcttt ttgaatattc cattatattt atcatatagg
     8341 aataaaaata ttgtgtaaaa ctttttttat taataaaaat aaatcttaac acaattttta
     8401 cat
//
LOCUS       DAWEJW010000109         8368 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2791_length_8368_cov_50.594250, whole genome shotgun sequence.
ACCESSION   DAWEJW010000109 DAWEJW010000000
VERSION     DAWEJW010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2791_length_8368_cov_50.594250"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1912 a         2417 c         1745 g         2294 t
ORIGIN      
        1 tttccctgcc ggcacacata cctaatttcc ttttttgttt cctacgcatg tgtttctttg
       61 acaattatac atactcaaat ctttttttca agctttatgc atgtatttca tagcattatt
      121 accttaaata ataaaaatcc gggagatgaa ctgtcccgga tttttgaagc tttatattgc
      181 ctgcaagcag tctcgctgac tttctatggt ggaattatga ttacaacttc cagtcggcga
      241 cctccatctg agtggctttc atatgccggt aaatgccatc ttttgcttcc agttcggcag
      301 gcgttccctg ctctgccacc acgccatcct tcaatacaac gattttgtct gcgtccgcca
      361 cggtacgcat gcggtgcgca atgatcataa cggtcttatt gcggatcagc ttggaaaggg
      421 attcctgaat cagtgtctcg ttatctacat ccagagaagc ggttgcctca tccagcagga
      481 tgatgggcgc atcctttaag aatgctctgg caatggaaat tcgctgtctc tcaccgccag
      541 acagttccga accgttctca ccgatcatgg tctgccagcc gtccggaagc ttctccgcga
      601 actcatcaca gtgcgccagt tttgccgcag ccagcacttc ttcatccgtc gcattctgct
      661 tgccgatacg gatgttttcc atcaccgtat tattgaacag cgtcacatcc tggaatacga
      721 tagcgtacag agacatcaag gtctccggat caatggtgga gatgtccatg ccgcccacgg
      781 tgatcttacc tttatggata tcccagaacc gggaagccag tctggacacg gtggtcttac
      841 cgccgccgga aggaccaatc agcgctgtca cttcgccctg ttttgctgtg aagtccacgt
      901 ctttgagcac agtatcctga ctgtcatagg aaaagcccac atgggaaaat tcgatatcat
      961 accccttagg atccatggtg tcggcaccgg tctggatgtc atgggacagg atctcatcca
     1021 aacggtcact ctgcacactg ctgctgatga tggccgccag attctgcaat gccacctgca
     1081 ggggatcata cattctggat atcagcagca ggaatacgaa gaaggtcaga atatcgatct
     1141 ctccccttac caacagcacg ccaccggtca gcgccacggc agcgatgccc agttttaaga
     1201 tcatctgggc actccccaca aatactgccg taccaagctc cgtcaccagt gcatgtttct
     1261 ccactgcacg gattttactc tctaagcctt ccatgtactc tgcctgtgca ttgttggccc
     1321 gcaggtcgcg taccgtttcc agacattcct gaataccgtc cgcacagtcc attttcaact
     1381 tcatctgctt cttgctcaga ctcttctgta ccctgccgga acagcccaca atgaggaagg
     1441 atacgggcag tacccataat gccgcgatag ccattttcca gttcaggaaa aaggtaccga
     1501 tggcgatcag tgtcgtagaa atgcaggagc ctgccagttc cggaatgaaa tgggaggagg
     1561 cggtctccat ggcggcacag tccaccataa tggtactggt cagatcggac aggtcctttt
     1621 ttccgaaaaa ggataacgga agctttcgca gctgctccgc cagggcaatt ctccgtaccc
     1681 cgctctccac ataagttgcc aggaaggtag tccggtactg gatcaacgtg gtaatggcga
     1741 tcagcgaaag acacaacaga cagccaatga aataaaacat acctctgcct gccatcgtcc
     1801 cctgcaaata atctcttacc agaaaataca gcatccatac cgggaacatc aataccagat
     1861 tggaacatgt cacggatata aatgccttta tcatatcctt tgcacctttt tcggaaagtg
     1921 catatttatg ctgcaatttt gcctgtaaac tgctcattct aatactcccc ctttcctact
     1981 ttccagctga cggatttgtt atatgcttcc cacatatggg catacagacc gccctgggct
     2041 agcaatgttt catgatttcc ctgttccaca attccgccat ccttcaacac acagatacgg
     2101 tcgctctcca cgacggagga aagtctgtgg gcaatcatga tcacggtctt gcctgcggaa
     2161 agcttcgtaa atgccttctg tacctttgcc tcattgtcgg ggtcggcaaa ggctgtcgcc
     2221 tcatccagga tcaggatcgg cgcatttttc agcattgctc ttgcaatgga cagacgctgt
     2281 gcctcgccgc cggatacata ggtgccctga cttccgatta cggtatccac tcccttcggc
     2341 agcttggcaa taatgtcctc acactgggca tttttcaaag cttccatgac ttcctctctg
     2401 gtggcatcct ttttgcccat acggacatta tcataaatgg acatcttcag cagcttactg
     2461 tcctgaaata cgaaggacac catatccatc aactgccggg aagggatttc cttcacatcc
     2521 acaccgccga tacagatctt cccttccgta gtatcgtaaa agcgggcgat caggctggcc
     2581 agggtggtct taccgccacc ggaggggcct acgaaagcca catgctcccc ttctctgatc
     2641 tccagattga tctgatgcag ggcatcccgc tctgcatttt catagcggaa agacacattc
     2701 tgcagggcaa tggaatgttc ccggatgttc tgttcttttt ccgcttccgg cagaggcttc
     2761 ctgttgagga tgctgtcgat cctgttcagg gaatcttcca cgagcatctg attttctccg
     2821 ctgtatgcga tcttatttaa ggtaactgtc agtaccggcg taatgatgat gtaaaatagc
     2881 aggttgatca gtaattccgg atctccccct gccccaccga accagtatgc ggcaccgatc
     2941 aggacagcga agatggcatt aatcaatgta gtaaagccta ccataggcca catcagttcc
     3001 agggtatagg caatgaccca tttttcatat tttttaatgg aggtctgaaa tcgtttgaaa
     3061 gaaaataccg actgtccgaa ggtcttcacc accgggatac cgcggacata ttccacggct
     3121 tcactggaca tttcctccag cgcattctgg tattctgtca tcttatcctt catgcgttgt
     3181 ccggtcatgg ctcccatgat gagaaatgca gataccacgg gtaccagact taacagcccc
     3241 agtttccagt cgaacgccac tagcaatact aacagtccta ccggcgttgc catggcacct
     3301 gccttatccg gcagctggtg tgccagatag gtctctgtgg ctgcggtgga ttcattgacg
     3361 atcttacgga tcttaccgct gccctcactg tccatgaatc ccatgggcag cgtcagaata
     3421 tgccgcatca gagtgcttcg catattggcc tgtacgtgaa aggcggcaat gtgggaacac
     3481 agcagtgcgc agatataaat gatcatggaa agcagtgcaa atcccacggc actccatcca
     3541 agccccgaca gattccgggc cttgttgtaa tccggtgctg ccaacaccac ctcctgcatg
     3601 atccgccaga tatcataaaa cggcagtaat gcaatcagtg cactgaccgc ggacagaatc
     3661 catgacacga tagtcaggta cttgtagttg cccgcataag cgaacagctt ctgcatagag
     3721 ctttcttttt tcatgtgatc ttttcctctt ttcctttgat tttatttgat tcttcaattt
     3781 ctaaaatttc ttttcctttc gcccggattt tcatcatttc tcttttccgg acatttgagg
     3841 ctctattgtc tttcctatct aacactgctt attgagaatt agtttcaact aaccgtcggt
     3901 ttttaagata gactgctacc gtaatcttcc atagcagtct tatcttatga ttcaccccag
     3961 cttgatgatg gctccccatc cgccatagtg atatttctcc agcatatgca gatattttct
     4021 ggcagtatcc ctgtccatgc ggtgtctgac gacttcgaac atgctgtcaa acatggctct
     4081 cgccatgata tgtaggaact cctcggtgat catgctgcct tcctgtagga attgtgtggc
     4141 ttccatatac ttgtaggtat actccgtctc aatgtccacc agatgttcca cgaaatcctg
     4201 atacttggta ccgtaagagg catccagcag taaccggaaa tcatcgaacc gatcatagac
     4261 atagtcaagc atttcatcca taccgcctac cacataatcc tccattacct tcggctgttc
     4321 ctctgcctct ctggcatgaa acgcctcctg ggtcttaagg aatatctgcg tcagcccttc
     4381 cgcagccggt tccacaatcg cacaaaacag tccttccttg tccccaaacc gggaatagat
     4441 ggatccggta gtagtgtctg ccttggctgc gatcgtacga agcgacgctt cggaatagcc
     4501 cttctccagg aattcttctc tggcacagac gagaattctc tctgaaactc cttcgattgg
     4561 ttttgtcact ttttcctcca aaaaaatatt cataacagcg ttatataaca ctgttatgaa
     4621 tataaacaca tttcattttt ttgtcaagag gaaaaatcgt ttcttactac tgcgtggtga
     4681 attctaccgg ctgcgcatct tttctgtatt cctcccatgc ctgcagatac tcctcctcgg
     4741 tactttccac acgctgtccc cggctgtcca gacgataata ggttgcattg ccgttctcgt
     4801 caaagctttc tgtattggaa ccggtcggta ccagctgtcc ggtcgcagta tcaagattgt
     4861 acacttcgat gctgtgcccg gaagtcacat agaacccggc agttccgacg cagatcggat
     4921 atgcggttcc ctcacttaag agcttgccaa tctgcaccgg tgctgcatcc tctccgatac
     4981 cgtagacatc acagtacatg gcataggtat gctctccgtc ctgataaatt ccgtccgata
     5041 ataacaacag agtcgtatca ccgactttta cctgcgcata cgatgcatct ccctttaccg
     5101 ctacaaagcc ggtggttgca ccatcctgca tattcatcag tgcggtagca ttatcattct
     5161 gttgtatcgg aacccagccg gcagaattct ccgatgcctg tttctccttc tctgcagcga
     5221 ttgcttcctt caccgcctct acctgttctc tctgtttatc cagtgcctcc tgcaatgcgg
     5281 cgtccgtctc ctgttgtgca ttgacttcgc ctacgcttgc gacatttccg gtatccacac
     5341 cgttttcgct ctttccggcc tcgttcggca gcaggggcgt cgtcccctgc tttggattcg
     5401 tcagaaaaca taccgctacg atcccacaga ccgccacagc ggcaagcacc acccaaaagg
     5461 tcggcttctt ataattcaat atctctttca ctctcttttt cacccctgct tctccaaagg
     5521 ccaacgggca taccgtcacc agccgccttt gtatactaca ggaaagcagt gcataggaat
     5581 agtcttttcg ctcctgagga ttcagcttct tcaccgcgct ctcatcgcat gccagttcca
     5641 gatcgttgca gaacaggagg tacgccaccc agacaaaagg ttggaaccag tgaatcgcta
     5701 aaatcagaaa tccgagcggc ttccaccact ggtcgccccg tttcagatgc gcctgttcat
     5761 gagcaatgac gtaagggtac agcacttcgt ccatactgga ggacagatag atcttcggct
     5821 ttcgcagtcc caggataaag ggggatttta cctcatcaca gatccacagg ttctcccgta
     5881 gccacatagc ttccgctacc tgcctccgta cccgggcgta actatatacc gcataacctg
     5941 taaaaaggat aacacctgcc agccagagaa tcgagaatgc agataatagc cgttccggta
     6001 ttctccatac tcctgctcca aatgctccga tgccacttcc ggtaattccg aaagctccgc
     6061 ccatgccgga tccggctgcg cccatgctgc cgtctaaagc tgcctgggaa atattgttgg
     6121 aaaaattagt tccgttttgc agcgtatcag cccggaacac atttcctgcc ggattgccta
     6181 tcatatttcc gtacaaatag cccgacaata tcggcagctt cggcatcaga ctgaaccggc
     6241 tctccggcac aaaagggcac accagccgca gtcccacaag cccccacagc acaagattca
     6301 cccacttcgg tgcccgtttc agcaagagtc tgagcaccat tacgacaggg atcatccatg
     6361 ccgcggcaat actcatctgt aatacacccg aaaatatgcg gctcattctt ttcccccttc
     6421 tgcctcatcg atcatcctac ggatcttcgc agcttcttct gcggtcagcg cacgatcttt
     6481 cgtaaatgcg gcaaggaacg caggcaatga cccctcaaag gtgtcttcca caaactgtct
     6541 gctctgcatc gcatagaact catccctgga gatcagggaa gtcaccgttc cgttgtcatt
     6601 gcgaaacagt cccttatcgc acagtctgcg cagcacatta tgcgccgtgg tgcgcttcca
     6661 gtgaaatgct tcctccgtca gcttcaccag ctccgaagaa gacaccggtt cgtgctccca
     6721 gatgatatcc gcataccgcg cctcgaccac acctaactga atctctgcca tgttgtttcc
     6781 tcctactact gttttcctga tgacaccaat tttgatattt cacgtttact tttgcaaaag
     6841 tatagagacc acatgctgtg gtctaattac agactacagt atgtggtctc tattgtcaag
     6901 tatcttttct attgcaaatg atatgtctgt ggccatcctg cccgttttcc gcactcgatc
     6961 tcccggccga tcctttcaaa gatatattgg tttttgtgct ccagatcgat cagctgcagc
     7021 atttcctcat ccgtcagctc acgctccggc tcatagaaca tcgcatcact gatgcaatag
     7081 gcaagctcct gtccatcata ttcttccgga tcagttatga ccttaattgg tgactctgta
     7141 taggcatttt ccacttcata cttttccgtc cattgctcat agcggctctg ttcctctgct
     7201 gtcaggttac gactgtctgt tccccataca tctactggtg cagaagccag taaggcatat
     7261 aattcatcaa tctcctcatc cgtcatctgt tcatatctgt caaaatagga aacctcggaa
     7321 tacttctgct gccatagttt ttcccgaagg ctgtagtcca gtttatgcag atactctata
     7381 atctccagcc actcttcctc tgtgagcaca gtatccggca gatagatcag acaaccgtat
     7441 tcttctccgg gagtacttcg gactgccaca ccagtgccgg aatagttctc gtcttcggct
     7501 agttcccgaa tctgtcccac cggatatttg ccatccgctt cgtattctct tgtcagctgc
     7561 tcataaatgc tccgttctct ggctgtcaac tctctactga attctgctgt gacattgccc
     7621 tgctccgcta ccatctccaa atgccccaat tcttccgtcc cgatgtcagt catgcgctct
     7681 gccagatagc ggcggtatcc catgattccg gcggcggtgg cggatactgc tgtgacagaa
     7741 agtatcacca cagctaacag gatgcctgct gcccggggaa agcttctgac tctcctgccc
     7801 gcaggactct cctgcgctgc ctgttgtctg atctgtgcat aggctgcatc cgccctatcc
     7861 agtacgatct gtggaagttc tatctctatt tcattcatcg gtctcatttt cttccgcctc
     7921 cttcctctgt ctccgcataa tatctcgcca actgctcccg ccctctggcg agccttgtgc
     7981 gcaccgtact gtcgggcagc tgcagtaatt ttccgatctc tgctgtccgc agtccttctg
     8041 cataaaacag taccacgatc agacgatatt tctcatccag tccctgcaaa gcttccttcc
     8101 attccagatt ggcctccgac tctactgtac tccccgtttc ctgctccgta aattcctcca
     8161 cacatacgac acgcctcccg gaacgtaaaa tgtctttaca tttgtttatc aggatgcgga
     8221 ccatccatgt cttaaaatac cggtttttct gtaaagtatc gattttttcc caacaagtga
     8281 gaatggtctc ctgtatggca tccgcaatat ccacatcctg cgcaaggatg gcactggctg
     8341 tccggtacat gtccttctca tgaagctg
//
LOCUS       DAWEJW010000110         8247 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2830_length_8247_cov_58.495483, whole genome shotgun sequence.
ACCESSION   DAWEJW010000110 DAWEJW010000000
VERSION     DAWEJW010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2830_length_8247_cov_58.495483"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2296 a         1807 c         2295 g         1849 t
ORIGIN      
        1 gcatgctccg ggagttcgtg gattttgcca atgtatttaa ctaactttct atttagtcgt
       61 tcaaagccat gtctttcaat ttcgcaaaca aattccgtac cggcttagcg cttaaaatta
      121 ccagagttat ggctgcttca gagaagatat agatagcatt gtaggcgagg gagtaaggca
      181 ggggatccca gccttcccag gcatagtagc cgaagaagat ccatccggag atcacggcaa
      241 acacatatct tccaagaacc ccggcaatat atcccttgat cagtcctagc ttcgcattgg
      301 agaagagacc ggacagtccc agagcgccga aagcaagaag atagtctacc accagctgca
      361 ggggatataa tacataggga tctatgagca gctgcaggac accgtaggct acacctgtgg
      421 caataccggc acccagaccg aaccagtagc cgggcaggca gatcaccagc atggacagca
      481 aagtaatgga gccgccggta ggaaaatgga acaatttgat attggaaaga acggttccca
      541 gggcaatggc catggcacag aatgccagct gctttaccgt aagcttgctg cgattcatct
      601 tatgatcacc atcggaaaca gtgggagcta cagcagtctt tttcatctgc atgcgggcaa
      661 ataaaacagc ggccgccaac agtgcggcca gaatgattac caacaggaca tttcccaggg
      721 tagtggggat ataagtagtc tctccccagt cattggtaac agcagtgtac aacataaaaa
      781 atcctccttc gtgtgcgcga ggagggacaa catcgtattc atacagataa actctctgta
      841 tgaataatgt gcttccttac gccggtatta tccggttcaa gtagtgaggg ttagaagtga
      901 agccttgatg gcggcacttc aatctcagcg atgtgctccc ctagcggata ttctatttac
      961 ctgtttacta tagtgcttta tcgcagcagt gtcaagcgtt tactttcgga atgccccggg
     1021 gaatttataa aaaggttttc aacacctgca acactccgtt ttcccaatag ggcgcacagg
     1081 tatcccttgc agcggcaatg acttcctctc ttgcattgga gacagcatag ctcattccag
     1141 cgctttgcag catttcgatg tcgttcagat tgtctccaaa ggtacaggtc tcatccggcg
     1201 cgatccccaa atattcctgg agaaaacgga tcgcagaccc cttatttacc ccaaggggat
     1261 tgcagtccac ccattctttt ccggcagaag caagcttcgc ccgggactgc caggtgggag
     1321 tgaagacagg tgcacacatt tcttcacagg cggaaggatg atatacagtg aacttgatca
     1381 cattgtccac gggcgttttc ataagatcgg cagattcccg gatgtcatat ccgtaggaat
     1441 ccgtaagcca ccggaacatc cggcttccag catcttctgc cagacaataa tccggtgtgg
     1501 caataaaata atcgcagtcc ggtaattcat tccggactgt ctcacacatg ccgtgccaga
     1561 tctcttcggg taaggtatgc acctttaaaa tttccttcgg tgtgcggacc acggtaccgc
     1621 cgtcggtgat atagaggagc ttgtcgcgga tgggagcaaa aagcttccgc tcactgataa
     1681 attgtctgcc ggaacaggca acgaaacgga tgccttttgc aatcagcttt tcaatgacgg
     1741 tcatgtattc aggatccagc atcagagtgc cgtccttcaa tagcgtgccg tcaatatcgg
     1801 ttgcaataag tttgatcata gtgcctcctg aatcttacat ttcattatat catgattcgc
     1861 accttcggtg cttaacgttg cttcgcaact atcatgatca acttcgccgt tcgccaatca
     1921 tgcaagcatg ttggctctct tgcgctcatt atatcatgag ctcacgaatc cgcgctatcg
     1981 cgctctacat cctgcttcgc aggatattcg ttcgctaatg agcccagaaa aacaaatgtc
     2041 tgcttcgcag ccgcttgttt ttccttgagg ctcattacat tatatcctac gcggtgccgg
     2101 atttgcaaac ggtatctgga aatacaaaac agttggaaaa tgctcatgga tatagtattc
     2161 tttataatag aaaaaacgtt ttatacgtgc ctgcaggatg atgctggcat tggaaggata
     2221 ggataagcat gaaaaaaagt atcgttggga tcctggcaca tgtggatgcc gggaagacaa
     2281 cattggcaga aagcctttta tgtgcctgtg gtgtcataga aaaggccgga agagtggatc
     2341 acggagatgc ttttctggat aacggggaca tggaacggaa aagaggaatc accattttct
     2401 ccaaacaggc gagaatccgg tggaaagatg tggatatcac actgttagac accccgggac
     2461 atgtggattt ctctgcggaa atggaacgga cgctgcaggt actggatgcg gcggttctgg
     2521 tcatcgacgg aagcgatgga gtgcaaggag atgtacatac gctgtggcgg ctgttaaaaa
     2581 gatatgaagt gccggtattt ttatttgtaa acaagatgga tcaaccgggg acgtatccgg
     2641 aaaaagtact gacggagcta cagaaaaagc tggatagcaa tattacatcc gtgggaaaac
     2701 tgctgcagcc tggggacaat gaaggagaac gggaaacaga gctggaacac gcggccatgt
     2761 gtagtgaagc cttgatggaa gaatatctgg aaaccggaga actgtcggcg gaaaacatat
     2821 cggacactgt ggcggagcgt aagctgttcc catgcttttt cggctccgcc ttgaaacaat
     2881 ggggaattac agagctgtta gacggattga atgcgtatct gccccaaccg gaatatccgg
     2941 aggaattcgg agcccgggtt tataagatcg gcagggataa cgcaggaatc agattgtcgt
     3001 atttgaaggt caccggagga agtctgcggg ttaaaatgcc ggtgggaaac agcagaagcg
     3061 gtgcaggaga ggaaatctgg gaggaaaaat gcgaccagct gcgtctgtat aatggcggaa
     3121 gctatgaggc agtggatacg gtgaaagccg gtgaggtctg tgcggtcacg ggacttacga
     3181 agactttcgc cgggcaggga ctgggctatg aaagcgagaa ctccacgccg atactggaac
     3241 cggtgctgac ttacagacta ctgctccctc cggaggtgga tcctgtcgtg gcactgggga
     3301 aactacgcca gctggaagag gaagaaccgc agctgcatgt gttgtggcag gaggaaaaca
     3361 gggaaatcca tatgcaggtc atgggacagg tgcagatgga aatcctcaaa aatatcctgt
     3421 gggagcgctt cggtctggaa gtggaattcg atgcgggaag cattgtatac aaggagactc
     3481 tggcggagcc ggtggaaggc atcgggcatt ttgaacccct gcgtcattat gcggaggtac
     3541 atctgttgct ggagcccggg gagcggggca gtggattgca atttgcctct ctgtgcagcg
     3601 aggacatgct ggaccgtaac tggcagcggc tgatcctgac gcatctggag gaaaaacgtc
     3661 atgtgggtgt gctgaccggc tccgaactca cggatctgcg gatattgctg attgccggaa
     3721 aagcccacac caaacataca gagggcggag atttccgaca ggccacttac cgtgcagtac
     3781 gtcaggggat gcgcagcgga aagagcattc tcttagaacc atggttttcc tttcgactgg
     3841 aggtgcctac ggagaatctt ggcagagcca tgtcggatat cacgaggatg aacggaagct
     3901 ttgaagcacc ggagacagaa ggcaataaca gtgtgctgac gggaagtgtt cccgtggctt
     3961 ccatgggaga ttatggaaaa gaggtggcat cttataccaa gggtcatggg cgtttgtcct
     4021 gcacactgaa gggatatgaa ccctgccata atccggagga ggtaatggca gagatcggct
     4081 atgatccgga agcggatgtg gtcaacccca ccggatcggt attctgcgct catggtgccg
     4141 gttttcaggt gtcctgggac caggtgccag aatatgccca tgtggagagc aactggaagc
     4201 cggagaagga aggaaccaaa gatacggacg gcatttcgga tgcgggactg caggcagtaa
     4261 acaggcagca gggcgctatt cgtcagaccg ggggtggtgt agagcggatt atttctcagg
     4321 aagagatcga agagatcttc cgtcagactt atgggaaaaa ggcggaggat ccccacagat
     4381 tccgcagata ccatacttct tccggaaacc gtggcagcta tacgggcagc ttggcgggaa
     4441 gtgccggtga catcggtatg cggaaggtcg taccgcagga aaaaccggag gaataccttc
     4501 tggtggacgg ttataacatt atttttgcct ggccggagct gcgggagctg gcgaagatca
     4561 atctggacag tgccagggac aaactgatgg atattctgtg taactatcag ggatatcagg
     4621 gctgtcgcct gatcctggtc tacgatgcct acaaggtgaa ggacaatccc ggctccacca
     4681 tgaaatatca taatatagaa gtagtctata caaaagaagc agagactgca gaccagtata
     4741 tcgagcgcac gacccatcag ctgggggcga agccgcagaa ataccgggtg acggtggcaa
     4801 cctccgatgc actggaacag atgatcatct ggggcaacgg tgccaccaga atgtccgcac
     4861 tggggctgaa agcagcggta gaggcagcag gtgccgaata ttttaaataa gagagtttta
     4921 ttggacttga aaaagtgtaa tctatacctg tcaggacggg aaaataagat ttccgacaga
     4981 accctgagca caaaaagcag aaaaagaggt atgggttatg agaagacagt tcgtttccaa
     5041 tgtggcattt tacagtacta ccgcagtact tattctgatc attgtattat gtatcggcgc
     5101 aaccgtgaag agcgagagcc gggatgagac gcagcgcatg gagaaccgga tacaggaaca
     5161 gcagctgatg agcgatatga agcagtatct gaatgagaac ggttatcgca acagcggcgt
     5221 gacgctgacc tatgcactgg aagaagacgg cagtccggat tatacgttta ccattcatca
     5281 taagaagatc aacagcatga gtgagacgga acggggagca ttgagcgcag aactggcacg
     5341 ggcctgcaac gcaggcggca attattcggt ctcttatgaa tatctgctaa cagattttta
     5401 gagactattt acatgacttt tttggaaata aggggtatac tgataccata tgccacagta
     5461 aacgaggcga tgggaaagga gaacgacaga tgacatttat tcccaaacca catgagatat
     5521 ataagcattt taagggcaat ctgtatcagg tcgtaacgat tgcggaacat tcagagaccg
     5581 gagaacagct ggtgatttat caggctttgt atggagattt caagacctat gcaaggcctc
     5641 ttgccatgtt taccggagaa gtggatcgtc agagataccc tgaggtgaca cagcgttttc
     5701 gttttgaatt acagggagcg gaggcggaca gacaggcaca cgaggcggac gcagtgagcg
     5761 ggacacagac ggcagcagtt cagacgaaga cagcaaagat gccgtcgact gcatcccagc
     5821 cagcagcagc cctggcaacg ccgattgtgg cgcagaacat agcagcccag gcaacggcga
     5881 ccgcggcaca gacaacaata gcaccggttt cccccgcaga agatgaagag cccgcactgg
     5941 atccgttggt actggaattt ctggatgcgg attcctacga ggagaagcta aatattctgg
     6001 caggactgca ccatcgtatc accaacgaga tgatcacgac catggcgatt tcctgtgata
     6061 ttgaagtaaa tgacggtgag ccggaagagc gttatgaaga attgaagaac tgcctgctca
     6121 ccatggagaa gttcgaatgc aaccgtctgc ggtagactct atggataaat acaatgaaaa
     6181 tttaacggat gttaaggaaa tctttaggaa aggtgacctt cgggcgattc ccaaaccact
     6241 tttaaaatgg tatgataata accgtaggat ccttccctgg agagaggatc ctgctcccta
     6301 ccgcgtatgg gtctctgaga tcatgctgca gcagaccaga gtggaagcgg taaagcccta
     6361 tttccagagc tttatggaga cgttgccgga tattgctgcg cttgcgcagg caccggaaga
     6421 ggtgctgtta aagctgtggg agggcctggg atattataac cgggtacgga atctacagaa
     6481 agcagccatc cagatcatgg aagattatgg cggagtcatg ccggattctt atgaggagtt
     6541 attaaaatta aaaggaatcg gcagctatac ggctggggcg gtatcctcta ttgcttacgg
     6601 caagcccaat ccggcggtgg acggcaatgt tctgagagtc attgcaagag tccgcaagga
     6661 tgaacgctgt atctctgagg ataaagtaaa aaaggctgtg gaaaaggatc tgtgggaggt
     6721 cattcctaca gacaggccgg gagatttcaa ccaggctatg atggaaatcg gtgcctgtgt
     6781 atgtattccc aacggggctc cgcactgtga ggaatgtccc ctacagcaga tatgtctggc
     6841 gtatgcggac ggtacacagc tgcaatatcc gaacaaagcc aaagcaaaag aacgtacggt
     6901 ggaggagaag acgatcctca ttatccggga tgcggagctg gcggcattgc ataagcgtcc
     6961 ggcgaaaggt ctgctggccg gtatgtatga attcccttcg atgaagggat atcataccgc
     7021 ggaggaggtc aaggaatatc tggcggagaa cgggttgcag gtactccgga tccagcccct
     7081 tgaggatgca aagcatattt tttcccatag agaatggcat atgaaaggat atctgattcg
     7141 tgtggatgaa ctggcaccga agcgtgaagg cccggattca gcagactgga tctatattga
     7201 accttcggag acaagggaga aatatcccat tccttccgcg tttgcagtct atgcgaaggg
     7261 attgaacatg aaactggggg cccgagagaa cgatcccgga cagtgaataa caagatagtg
     7321 aagaaaggac agcgatatga aattactttc catagcaatt ccctgttaca attctgaaaa
     7381 gtatatgaga aaatgtattg attccctgct gatcggcgga gaggatgtgg agatcctgat
     7441 catcgatgac ggttctacca aagaccgtac cgcagagatt gcagacgaat atgaagcaca
     7501 gtttcctacg attgtgcgtg ctattcataa ggagaacggc ggacatggtt ctgctgtgaa
     7561 caccggaatc gccaatgcca cgggactata ttttaaagta gtggatagtg atgactgggt
     7621 gaagcaggat gcatatttta agatcctgga tactctcaga gaactggccg gcggcggtca
     7681 ggcattggat atgctcatca gcaactatgt ctatgaaaaa gaaggcgagc gccgcaaaaa
     7741 ggtgatccag taccgtcata tcctgccggt ggagaagatg ttcacctgga cggactgcca
     7801 tcattttctg aagggccatt acatactgat gcactccgtg atcttccgta ccagactgtt
     7861 acaggaatgt ggactgaagc ttccggagca caccttctat gtggacaacc tgtatgtatt
     7921 tgaaccgctt ccttatgtga agaatatgta ttatctggat gtgaatttct acagatatta
     7981 tataggaaga caggatcagt cggtgaatga gaccgtgatg atctcccgca tcgaccagca
     8041 gatccgagtg acgaagctga tgattgatta tctggtgggc agaaagtcgg aactggtgaa
     8101 aaaccgacgt ctgtaccagt acatgcgcaa ctatctggag attatcatga cagtatcttc
     8161 cgtactgttg atccgctccg gtaccacaga acatctggag aaaaagaaag aactgtggga
     8221 gtacctgaaa ggaaaagaca aacgcct
//
LOCUS       DAWEJW010000111         8239 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2835_length_8239_cov_59.238636, whole genome shotgun sequence.
ACCESSION   DAWEJW010000111 DAWEJW010000000
VERSION     DAWEJW010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2835_length_8239_cov_59.238636"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2217..2316
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2191 a         1895 c         2087 g         1966 t
ORIGIN      
        1 atttaattcc cgacccgatg caatgtcaca tcatccagag ttccccagct gttcttatta
       61 cattgatatc tcacgccaat ggtcagggtg ccgtcggtga ctttgatacc ggagacggtg
      121 ggattctgcc agtcggcata ggtggtgagc ataaaaggca ctgtcagttc cttgccgtct
      181 acgacggcat acagctccat ggaagcatct gctgccaggt caccgccctg tgagaaagcg
      241 gacaattcat aagtaccggg ttccagatct gtgaagctct gctcaatgga aaagtccata
      301 tcggaggtac cggaccagaa gtggaaagcc acctcaccgc tgtgggcatc ggcagctttg
      361 acctgatagt cggtgggatt cgtttcaccg gagtatgtta cagtccacat agaagtatca
      421 gcattctcaa agccgggatt ctgtacataa ttgacccgtt ctaccgtgac aatggcagta
      481 acttccgtac cttcatccag agatccggca actgtgaact ctccgccgtt ttccgtatcg
      541 atggcggcaa tatcttcagc atcccaggtg acaggaacct gttcttcgga acgatcgtta
      601 tagatgatac ttacggtatc cggaagcttg acttccgcac cgatattgca ggacaccact
      661 atttcgggga tggtgtctat cgccagagga gcagtggctc cgtacttcag atatttaaat
      721 atatttaggg aggccagagg atgcccggtg aagtcgaaca tggcctgatt gtcccaggag
      781 cttccgccgt aatactgtcc ggcatccttt ggatcataac cgcctgcata actgctggcc
      841 caaccgctgc cgtatttttc ccagatggca gaattatcag catcggcggg accgaccggg
      901 atccaggtac cttcccagta gaagatgcct tcggcaccgg cctcgctggc agccgcacag
      961 acatcccgca cttcgttggc ctgcccctgt acgctggctg aatatccttc tgccagatca
     1021 tcggttccct tgatactgtt ggcagagcca tcgccgtctt ccgaagtata acagtaagcg
     1081 ttttccgcta catatacctt tttgccataa gtatcacgga catggataat ggcatttttc
     1141 agatcgtcca tcgtgccgtg ccagtaggga tagaaggaaa gacctacaat atcataatcg
     1201 atttctttta cctgcagacc ggtcagcagt gtatccagct ttttcatatc atcgatgttg
     1261 gtgtaatgta cagccaccag aatatctttt ccggaagcag tggcagcatc cctgacagcc
     1321 ttactgccgg aggccaacag ttttcgaacg tttgatgcat ctgtctctcc acacatgccg
     1381 ttgttgatct cgttgcccac ctgtaccata ccgacgtcca caccggcatc cagaatctgc
     1441 gtcaggtttt ccagagtgaa gttatagaga gcgtcggatt tttcttcgat atccatacct
     1501 tcccaggcct ttggaacgaa ctgcttcttg ggatctgccc agaaatcgga ataatggaaa
     1561 tcaatgcaga ccttcatgcc atactgcgtg gcacgttgtc ccagagtgat ggcagtcgcc
     1621 acgtcattgt tgccgccacc gtaaccgttg ccgttctcat catagggatc gttccagacc
     1681 cggagacgga tatagttcac accagcctgc gccagcgtca taaatacatc ctgctcttct
     1741 ccgtcaaagt tataatattt cacgccactg ttttccagag ccaatactgc ggatacgtcc
     1801 ataccacggt aaaaatcatc ggaaatgccg tccaccggct ccacataaac ggtagactcc
     1861 tccggccctt ccggctggga aaaagtgatg acatccgtgg catctgcagt agcggtaact
     1921 gcggattctg ccactgtatc tgcgatttcc agatcctgac tgccgcatcc gggcagccag
     1981 cccatgacaa gtcccgcact gagatacaga gtcaacagcc gtgtacgtaa ttgcttgcta
     2041 aaatgttgca taagtaatcc tcccacaaaa cataatcgaa caaccgcttg cgcatctata
     2101 ttatagcata ttatcatcca gcaaagataa aaatgtaaca aaaagtgcgc aataatttcg
     2161 agggctattg cgcaaaacat ttgttttaaa taccagaagc agggaatcgt agatgtnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggc tattgcgcaa aacatttgtt
     2341 ttaaatacca gaagcaggga atcgtagatg ttggaaacca tgacaaagaa ccccggaatt
     2401 ctggaagcgg tgaaagaggt aagacatatg agtctgggaa gatgggcgag agccatgtac
     2461 gatggacata tgaaaatggt gcgggatcag caggccaggg atgcttatgt ccgggacagc
     2521 ggtgcagagc aggaacgcgc cagactgtcc accttgattg ccagcatgga tgctgcaggg
     2581 gagggagatc aggttgcccg tttgcaggat ccggaattcg tacaggaaat gttaaaaaat
     2641 atgatttgta aaagcttctg tgaagaagcc ggaccataaa aatacgccat ggggaaggaa
     2701 tccttctcaa tggcgtgttt tattacaagg cattatacaa acaattcgat cacaaacacc
     2761 agcacacagc agatcacgga taccggaaac agtccggcac cgagccatgc ggcgatgata
     2821 ccggcaatga gtgccaccag tccggaaatg ggggtctggg tagcatggat gatggccggg
     2881 aaggtcatga cggccagcgt cacgtaaggt acatagtaca aaaaggactg aataaactga
     2941 ttcttgatgg gcttgcggat cagtgtcagg ggcaggacac ggatcgtata ggagacagcc
     3001 gccataacag caatataaat ataattattc atttacgtgg tctcctttct gtacagattc
     3061 tccgttttcc gcaggaacgg cttcctctgc atcctgcttc accgggaaca ggattgctgc
     3121 agcaccggag atgatcacgg ttaacaggat ggttctcgta ccttctgaca gggaagaaat
     3181 cccgggcaga tagttgcagg caaagctcag tgcgaagctg attgctacca gagctgccac
     3241 gatcttactt tttctggcag gcggaatgat aatggccaga aacattccgt ataacgcaac
     3301 actcagggcg ctgacagcac gaagaggcag cgcatttccg gcgatgatac cgcaggcggt
     3361 tcctccggcc caggcgggcg ctgccaacag gatcgcaccg taggtatagt acggattcag
     3421 gatacccggc cgggcaatgg taatgccgaa caactcatcg gtgacatcgt aagcaatgat
     3481 cagacggtgg aaaaagggcg tgctgggaga aaatttctgg gccagcgcac aactcatgag
     3541 tagatatctg gcattggcaa tcagcgtgat gatcgccact tccagatatg tggcctgtgc
     3601 cgcaatcagg gtaaaggctg catattctcc ggcggaagcg ttatttaaca ggcttgccag
     3661 aaaaccctgc atgggggtaa agcctgcatg tcttgcggca attcccaaag aaaatgatac
     3721 tgcgaaatat cccagagcaa tgggaaagcc gtcgcggata ccgtcgcaga agactttcct
     3781 gctgaattta tcattcataa ttatgattcc ctcatctttc tgtaaggcgt cttttgacgc
     3841 acatcaccta ttataacgca cattttcaaa ggagaaaagg atgaaaatta atgagatagg
     3901 tacagaacag gagtatattt ctgctttttt cacataaata tgcaataagg aagttttaac
     3961 ggtggatgac ggatgaaaat caaatggaga cagtggaaaa tcataatgac cgcagtactg
     4021 ggggcattgc tgctcacagg ctgcggtctg ctttcgaaag agaatgtgaa acttagagat
     4081 ctggatttta cggtactcag tgaagaaaaa attcccgagg aattaaagac agtaatcgct
     4141 gaaaaaggaa cggagccatt tcagctgacc tacagtgata atcagaacct ctatatcagt
     4201 attggttacg gtgagcagaa gaccggtggg tacagcattg ctgtggatgc cctgtatctg
     4261 acggacgatg ccattcatgt cagcaccagc ctcttaggcc cggacatcac cggacaaaag
     4321 gaaggcgcag ggaaaccctc cacgccttac attgtgttaa aaacagaact tctggataaa
     4381 accgtgcttt atgattaaac gaatggttct tacagatcca tcaaaatctc ccggcgccgc
     4441 cagctgataa aaccatacat atcattggtg gcaaagatca gggaagtgac gcacaccagc
     4501 gcagccagca aagttaacgg gtcaaaattt tttcctcctg aaatatgatt tagttgccag
     4561 caacagactc attttcagga tccaggattg gcagattacg ctgacagcgt tcccgcagat
     4621 aactgcagaa agtagcggta gcccggctct gactgcggct tttgtcccac agcattttta
     4681 cttcgcggga cggtagaggc acatttaagg tcagcggaac gagactgtcg ttttcaaaaa
     4741 agggagctgc cagaggctcc ggcacgaagc ccactcccag attgttctgg agcagaggga
     4801 tgaccagatc ggcggtagct acttccatgg cgggttcgta gtccagatgc tgttcaataa
     4861 ataccttccg gtaatattca tagctggcgg tgccgtatcc cagaccaatg atggaatgga
     4921 gccggaattc tttcagcgtc atgggacgat cagacaaggc ggcatagtcg gggccgccgg
     4981 cgggaacctc ccggaaagtc agcagatctt cgcaggccag agcatcatga agttcaaaag
     5041 gggttgtcac aacagccaga tccagccggc cgttctgcag agatttcaga atctccggag
     5101 tggtattgtt ctggatacgt attttgatct ccggatagcc agtctggaaa tcatggagat
     5161 tctgtaacag aaataaatgc agggcggtct cggtgacacc cagttcaatg gtgccgctgc
     5221 acacgttttt gtcccggcac agttcggctt ccgcatccaa cagatgttga caggcgatgg
     5281 caacatgggc atagagccgt tttccctctt cggtcagctt cagtccccgg ggttcccgga
     5341 gaaataaacg gcagtccagc tgatcctcca gaatatgaat aatccgggtc acattaggct
     5401 ggctgctttt taagaccatg gcagccttgg tgatattttc gtattttgcg acataataga
     5461 aaatcttgta atattcaaaa ctaatatcca tataaaaatg atatgcctca catataaact
     5521 atatattttt agtatccgtg tggatatagt ataatacgcg gttgaaaaaa agtaaacaga
     5581 ggagaaaagg atatgcgaca tttgattgat tcactggacc tgtcggtagc ggagacgcag
     5641 gaaatcttag acctggcaga ccggattgct gccgattccg ctgcatatgc tcattgtgca
     5701 gatggaaaaa tactggcaac cttattttac gagcccagca cacgtaccag attatctttt
     5761 gagacggcta tgctgaacct tggcggcaga acattgggat ttgccggtgc ggaacagtgc
     5821 agtgccacga aaggtgagac ggtggcagat accgcacggg tggtcagctg ctatgcagat
     5881 atcattgcca tgcggcatcc gaaagaggga gcaccactcc gagcttccat gtattcccgg
     5941 attccggtga tcaatgccgg ggacggagga cataaccacc cgacccagac catgatcgat
     6001 ctgctgacca taagacagcg caaaggacac ttggatcatc tgaagattgg cttttgcggt
     6061 gacttgaaat tcggcagaac cgtacactct ctggtgaata gtctggtgcg ctatccgggc
     6121 aatgaatttt atttcatttc tccggaggaa ctgaaggttc cggaatatct gatcgaggat
     6181 actctgaaac cggccaaagc cccctaccat gaggtatcca gcctggagga gacgctgcca
     6241 gagctggatg tgctttacat gaccagagtt cagaaggaac gttttttcaa tgaggaagat
     6301 tatatccggt taaaagatat ctacattttg gatcaggaga aactgaatct ggcgaaagcg
     6361 gacatgccgg ttcttcatcc gctcccaaga gtggacgaga tcgcaccgga agtggatgca
     6421 gatccgagag cagcgtattt tcagcaggta ttgaatggta aatttatccg tatggcattg
     6481 atcttaagtc tgttagacct tacggatcct gtgacgggaa agaaggtgtt tttatgttaa
     6541 acgtagaatc cattcagaat ggtgttgtga tcgatcatat tcccgcggga aagggaatgg
     6601 acatctacag attgctggaa ttggaatcca agacgcagtc tgtagcattg ctgcaatcgg
     6661 tgaaaagcca gaaatacggc tgcaaggatc tgatcaagat tgagggcgag acgttgccgg
     6721 agcggttgga tattctggga tatctggaca gtcagatcac actgatcgtg atccgggacg
     6781 gaaaagttgc aaaaaagtat catccggttc ctccacaaag actggttaat gtgataaaat
     6841 gtaaaaatcc ccgttgtatt accagtatag agacgaattg tgaccacata tttgaactgt
     6901 caccttccgg aagatatcgt tgcctgtact gtaatcagga gatgcctgta ggaaggtaat
     6961 tgtatgtatc ggagacagaa tctgcaggtt actgaaagga atgagaatta ctatgcaacg
     7021 taaattacac ggacaaatgt cagagtcgtt tctgattgct ggaattttgt caatttccgg
     7081 cggattacag gatgcttata cttatatgta ccgtggcaag gtattcgcca atgcccagac
     7141 cggtaatatc gtgttgctgg cccagaatct ggtagaccgg aactggtccg tggcgcttcg
     7201 gtatttttcc ccgcttttgt tcttcgcgtt gggagtagcg gcagcggagt gcatccatat
     7261 gcgatatcag aaggctcaga ggatccactg gcggcagctg gtgcttgcta tagagattct
     7321 gttgctgttt gctgtgggat ttttcccgaa tgagatggat ctgctggcca atgccatggt
     7381 atctttcgcc tgcgccatgc aggtacaggc tttccgcaag gtgaacggtt atgcttttgc
     7441 cagtaccatg tgtatcggta atctgcgcag cggcatggaa tccctgtgtg cctatgggaa
     7501 gacgaaggac aaaaaaatct tacggaaatc cggcaattat tttggaatca ttttcctgtt
     7561 tgccgttggt gcggcgctgg gcggacatat gatcggctac ataggggcga ggaccatctg
     7621 gatctcctgc atcatattac tgatcagctt cctgtgtatg ttcattaaag aagaaaagaa
     7681 ggaacatccc gagatcgtac aggaggaacg ggagattcgt gaaaatctgc aaaacatccg
     7741 caaagaagca aaagatgtgg aacatattct ggaggatgat atccgttccc agattgagaa
     7801 gtaaaagtaa aaaaactgca acgaaaaata ttacagtgct tttacatcgt cccgtaaagc
     7861 accggtcatc tcctggacaa aagtggtctg ggaggagatc tcttccgcta cgacggcgaa
     7921 tccgcggcta tgttctcagg cacgggtcgt ctcgatggaa gcattcaggg acagaagatt
     7981 ggtttttcac accgcagtat ggccttgctg acatggtaaa aaatcggcca gaaccgatga
     8041 aatttgccgg cttccggtat catggacgaa aggctgcgat gaaaaaagtt tcacagcgat
     8101 ataccgaatt tctgcaggtc tactttattg ccggtgacct cgatgccgtc ggcccggagt
     8161 cggttggcct gctcgttgga accgccgaag gcaaaggcac cggagagttc ccctttggaa
     8221 ttgaccacgc ggtagcagg
//
LOCUS       DAWEJW010000112         8188 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2858_length_8188_cov_55.407722, whole genome shotgun sequence.
ACCESSION   DAWEJW010000112 DAWEJW010000000
VERSION     DAWEJW010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2858_length_8188_cov_55.407722"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2528 a         1634 c         2109 g         1917 t
ORIGIN      
        1 tgaacgtatt ttaatcatta cgaaggatga cactcttgtg tgcatggcat acaagggtgt
       61 tttatttatt cataggaaat tttttcctat gaataaataa cgctccgcag ggatcgcact
      121 gcggcggtta tgaaatatgt cgcaaaagcg acccgccgca ggcggagaat ctcgcaagcg
      181 agatcctttt ttatattgag agaggttaca tgagtaataa cgaaatcaga gagaagagaa
      241 aaaaagtaat ctgtgatctg gtgaaggatg atttttacgt tccgatgaag gaaaaggaac
      301 tggcaatgtt tcttcaggtc gcaaaagaag acagggaaga gtttcgtgag attttacggg
      361 aacttttagc ggaaggaaag cttaccctga ccgttaaggg taaatatatg aagtccaacg
      421 gtaaggttct gacgggaact tttatcagca atgccaaagg ctttggattt gtggaagtgg
      481 aaggaaggga cgaggatctg ttcattccgg aggacaagca gggtggagca ttccataagg
      541 ataccgtgga agtggcactt ctgcctgcga agaccggaaa acgccaggaa gcgcaggtta
      601 ttcgtatcat tgccagggga atgactcagg tagtgggaac ctatgagcag tccaagagta
      661 attttggctt tgtcattccg gataacacga agatagcaca ggatattttt gtgccgaagg
      721 agtggtcgaa gggggccatg accggacata aggtcgtggt ggagatcacc ggatacggca
      781 cgaatacgaa gagtcctgag ggcaaagtcg tggagatcct gggacatatc aacgatcccg
      841 gtgtagatat catgtctatc gtacgtggct ttgatctgcc ggtggaattc ggcgagaaga
      901 tcatgagcca ggtggagagg gtatcccagg aggtatccga ggcggattgt gccggacgca
      961 gggatctgcg ggatgtgacc atggcgacca tcgacggaga ggatgccaag gatctggatg
     1021 atgctgtcag tgtatccttt gacgggacat attatcactt aggagtacac attgcagacg
     1081 tgacgaacta cgtgcaggag aattccgcac tagacagaga agcattaaag cgcggaacca
     1141 gcgtctatct ggtagaccgt gtaatcccca tgctgcccca tgcactgagc aacggtatct
     1201 gttccctgaa cgaaggcgtg gaccgtctgg cattaagctg cctgatgaag gtggatgaag
     1261 agggagaaat cgtggactat gagatctgcg aatctgtgat ccgggtcaat aagcggatgt
     1321 cttataccgt ggtgaaaaag ctgctggagg agccagagtg tgtagagcat aacgccggta
     1381 tgccggacgg aacctccggg gaaggcgtag aaacctcaac agactattca cagtacaggg
     1441 aactgctccc tatgttccaa caaatggctg agctcgcgga caaactgcgg aagaagagac
     1501 agaagcgggg atccattgat ttcgattttc cggagtgcaa gattctgtta gataaggaag
     1561 gacatccact ggatattaag ccttacgagc ggaatatagc gactatgctg atcgaggatt
     1621 tcatgctcgc agcgaatgag acagtggcac agcattttta ctggcaggag ctgcccttcg
     1681 tctaccgcgt ccatgatgta ccggatccgg agcggatcca gaagctgtct acgttcatca
     1741 gcaattatgg ctattacatg aaggcgctgg ggcgcaggaa cagcaaggtc tccacggagg
     1801 agatccatcc caaggagatc cagaagctgt tacagaagat tgagggaact cccgaagagc
     1861 ctatgatcgc cagactgacc ctgcgcagca tgaaacaggc aaaatacagc acggagtgta
     1921 ccggacattt cggactggcc tgtcagtatt attgtcattt tacttcgcca atccgaagat
     1981 atccggatct gcagatccat cgtattatca aggaacagct gcggggacgt ctgaaggaag
     2041 agcgcataga gcattatcgt gagattctgc cggaggtggc gaagcattct tctgaaatgg
     2101 agcgccgtgc cgatgaggcg gagcgggaga cggataagct gaagaaggta gaatatatgg
     2161 aacaacacat cggagaggaa tacgagggtg tgatctccgg tgtcaccggc tggggactgt
     2221 atgtggaact gcccaatacc gtggaagggc tgatccatat ttccacgatc ccgggcgact
     2281 attatcacta caatgaggca gcctgtgaga tggtgggaga agccaccgga cgttgcttca
     2341 aactgggaat gccggtgcgg atcgaagtgg aagactgtga ccgttttatg cggaccatta
     2401 atttccggct ggtggataaa taaacctatg aatcaaatga actgttagtt cattgacaaa
     2461 tacggaaaat atagtaaact ataactattc agagccgtta aaaagaagct ccgcaggtgc
     2521 gccttgtgaa tgggtttctg aatagttaca aggttttaag aggtagccat ggcaaaagga
     2581 aaagagtccc agaagctgat cgcaaacaat aaaaaagcat atcatgattt tttcattgat
     2641 gagacctatg agtgcggcat tgccttgcac ggcacggaag taaagtccat gcgtatgggc
     2701 aagtgcagta tcaaggaggc gttcgtccgc attgaagacg gagaagtatt cgtctacgga
     2761 atgcatgtaa gtccttatga gaaggggaat attttcaaca aagatcctct tcgtgtgaaa
     2821 aaactgctgc tgcataagta cgagatcaac aaactgctgg gcaagatcaa ggaaaaaggg
     2881 tatacattag tccctttgca ggtatacttt aaagacggta aggtaaaggt agagatcggg
     2941 ctggcacgtg gtaagaagct ctatgacaag agagaagcca ttgccaaaaa ggatcagcgc
     3001 agagaggcag agaaggaatt caaggtccgc aatttgtgat ccacaggctc tgatcctgca
     3061 atgctttacc gcaagtgctt gacagtgtgt gtgccatcaa gtatacttgg tctgacaact
     3121 gaataagggg tagtaaaggt ttcgacaggg gttttgcagc tggagaagcg agtcgcatgg
     3181 taatgcgtta aatggccaaa ttaaaattaa acgctaacga taatttagca tacgctgcct
     3241 aatggtagca gtcggcccta gggcactcac accttaggat cccggcttca aacatgtgag
     3301 aaacgacata tgcaaagctt tgcgcatatg ggcgtaacat gaagctactg aaatgctcag
     3361 ggtgtttatt cccggggcgc agagggaatg tcaaaaaata aactacactc gtagaaggac
     3421 agggaatagg cttttggaca cgggttcgac tcccgtctac tccactaaac cgtaacaagt
     3481 cgaacaccct gtataacatt gtggtataca ggaatatgtt ctgcttggtt tcacagaggg
     3541 aatggtaatt gtgagaacgg ttattgtaga tagaatctac aggaaagcag cgatattgta
     3601 ggtaggtgat tacacctcat acagtatcgc tattttttgt agaagcatcc ccatgcctga
     3661 cggcacagaa gaagtaggga tgctttgaaa tttttagaac agtgcaaata aaagaagaat
     3721 atatcaaagg gcaagccgcc aggaagatgt agagcaatct attaacatct tcctggcggc
     3781 ttttgcattt gtcaatgccg ggagagacca tgaaggaggt aacggcaatg aatatagatg
     3841 ttgtagatgt gagactggat gaaaggaaac tgccgtatct ggtcagtgag acggtggcag
     3901 aataccgagg gggatatgtg ggagagcaac ggctgaaagt aaattccccg gagaaagtgg
     3961 taaacgtgct gaataatgtt tttcacatga aagatttttc ggaagaaaaa ctgtatgtga
     4021 tgttcttatc cgccagtctt catgcgatag cctatgcaga ggtcagtcac ggagtaattg
     4081 attcagccac cgtaggaatc cgggaaataa tgcaaagagc gcttctgacc aatgcggcag
     4141 gaatcattct ggcacataat caccccgggt tgggtagtac ggcgaatcca tcaacgcagg
     4201 acattgatgt gacacagggg ttgatgcgag cctgtgaaat tatggggatt catatgtttg
     4261 accacattat tgtagcagga gacgattatt acagtttcag agaaaagcac gcagaacttt
     4321 acgtgaagcg tgaggaaagc aggaaaaaga ggggagggaa acgaattgag atatagtttt
     4381 tgaatgattt ttataaaaaa tatcgacagg ggcgttagct cctgtcgttt ctattttgtc
     4441 ttatcatgtt cgataatgtc agaaacagga caatcgagga tgtagcagag cacatcgagt
     4501 gtctttgtag tgatggcttc tccctttttc atgcggtgga tggtattggc ggaaacaccg
     4561 tgcttaaaga tcaattcata ttctgtcaca tttttgcgaa gtagggtgtc aaaaaaaggt
     4621 ttgtatgata tcatgttatt agattgaacc tcctatcaca tattataaaa agagtatcat
     4681 acttaatttc ttgactatac ctaatatatt gactataatg taagaacgat agagaacgat
     4741 cacatgaaaa caaaagttaa tcgctcgaaa taaattctct caacgaaagc gaggaaaacc
     4801 aactatgaaa cacagaacac acaggctcgc tgcgaaagcc gtagcagcaa tcctttgcgc
     4861 agcactgatg ctgaccgcat gcggcagcaa agagacaacc agtaacgaaa ctgccagcac
     4921 agagactacc tctgtagaat ccacagaaat gacaaccaca gaagcagccg tagcagaacc
     4981 cacggtagag cctactgaga ctcccacaga atcagaagct gttcccacag agactactcc
     5041 tgcagaatct cctgcacctg ccgtggaacc cacaacagca cccgtagaac ccactgctgt
     5101 aagcacaggg tatacttatt cggaattgaa ccagaccatg tatgcaaagt ccgcagtaaa
     5161 tgtcagagac ctgcccagca ccgatggtaa gaaaattggc agcctgaaag ccagccagga
     5221 aattgtcgtt acgggtaaat gtgaccagac aggttggtat cgctttgaat taaataacac
     5281 cactggctat gtaagtgaca agtacatcgt atccgaaaaa cctgctgtga ataccgttgc
     5341 agcaaataac acaacagctg gtaacagcaa tactgctgga aaccagtatg caagtaatac
     5401 aggaactgat gaattcggta tctctgatgc tgttatcgag aacttcatga aaactcacgg
     5461 tgacggcggt actggaaata cagccactac aacaagcaaa gatgcatact tcgacagagc
     5521 catggcagaa caggtatttg caatggtcaa tgccgaacgt agtgcagcag atatccccga
     5581 actgacatgg tcagaagacc tgtacaatct tgcagtagaa agatgtaatg cagataatgg
     5641 acatgcaggt gccaccacag cagaaaatta tcaggcagga cagcctgacg aagtaagcgc
     5701 atccttcatc caccagaaat ggcatgacag ccagggacat tatgacaact acatggacag
     5761 cagacatgta gctggtgctg tagcaataaa gagaatcccg ctgaacatgg gtggactgga
     5821 tgtgaatgac accatcgtag catacgaagt attcacgaac gcagactctt ctgttgcatc
     5881 ctgcacagaa cctgtcgtga cggaaaacac cgtcgcagca gctcctaaga ctgccgacga
     5941 gaactgacac gtcatcctcg aacgtaggct ggtctatgac cccacccacg caggaagacg
     6001 gcttgaagaa gtggcaggag cagaaccctg atgtggaagt gtcgttcgga aattaaataa
     6061 caagtgccaa ggcggacgct gatgcgtccg cttttcatat tttaatgaag gtctgatgca
     6121 cagaccgggc gcaatacgaa aaagaaaggc tgtcgttaga cagaatggta aagaattatg
     6181 gatcgagata tggacttatg gaatgctcta cagaagcaca tggggcataa tgtaaaaatc
     6241 acgttcgagg gtgcggaaga taaccccgaa aatatcttac tgcaatgtga ggactgccat
     6301 ttcgatattt tgagtgctga cattgagacc gtgtgtgcga gagaggattg ttgacaaagg
     6361 aataaggaaa agcataccgc aaagaataat agcatactcc attgaccgca taacaaaatt
     6421 tgtgtataat tttactatca cagaaggagg tgcttttatg ccgaccatta aatcaagtgc
     6481 tgacttgcag aacaattata atgaaatatc gacctactgt cataactacc cggaacctgt
     6541 ttttattacg aaaaatggaa aaggtgacct tgctgtaatg agcattgagg catacgaaga
     6601 attaaccagc cgctttgaac tatatagcca gattaaggaa ggaatggatg atattgccgc
     6661 tggcaatacc agaccgtttc aagaagccat ggctgatatt cggagccgcc gcagaagatg
     6721 aattatcagg tacacatcac caaaactgcg gagcatgaca ttgcgaaagc agcagattac
     6781 atcgaatttg tcttgaagaa cccacaggca gcagaccatc tgctggatgt tgcaacggaa
     6841 aaaatcggcg aactgaccca gatgccggag aaactacagc tggtggatga tcccgttttg
     6901 gctggttggg gaattcgatt tattagggta aacaattatc ttgcgtttta taccatagac
     6961 gaagacaaga aggtggtaat cattgtacgt tttctttatc aaaaaagcaa ctggaacacc
     7021 attcttcgac aaggattttc actcatttaa ggaacctgct tttgcaggtt cttttttgca
     7081 gtccgccgga ctgccgacag ggtgcttgga aggagtgtgg caaatatgag gaagagaatg
     7141 ctttcaggta tcagagactt attgattgtc ctgttctgtc tggtggagag aacgttatta
     7201 cagattcttc ttgcagtacc cgttccggtc agaatagttc ttatgggaat actgggagca
     7261 aagtatttgg agtgtctgtt ctgtacaagc cttagtatga caatccttac cgttgtgatg
     7321 acggtctttg tcgcaacaaa tatcaagaag tggaacgcag aatttcagat catatatgtg
     7381 acggcaagac tcagaaagcg ttcacggaaa aaggcatata atttcagaat aaactagaga
     7441 atctacgggc agccgaaagg ccgcccattt ttttagccag ataatgtacc atctttggga
     7501 gcagaatgag ccacggattg taccaaaaag gaaagaacag gcattcccta catttgtgta
     7561 ccaatatgcg cccaagattg acccaaatct gggcggaaac cggtacagag aatggttcgc
     7621 ttttgaaaga aaaaggcatg acacgataca cagaacgacc ggcagcggtc acatgttttc
     7681 agaaggttcc cgtaagggaa cttatttttt agaataagct gcgcagaacc ttacggtgca
     7741 gtctaaggtc tgcgtaaaga actttcttgt aaacattgtt tacaaatttt gcaaataacg
     7801 tcccgtttac acgtcgtcac agacccttgc ggcttgtaaa caagttcact taaaaaatgc
     7861 ccgttttacg ggcgttctgg cactctcgtt tacagttgta aacaatgttt acagaaaagt
     7921 gccaatattc tgacggctcg tttacaggct caaacccgca tggttacgga gttttcgcag
     7981 aaatctcatt gctttctccg ctaaaggctc cggaaagcaa aaagggcata agcagagctt
     8041 gtcacatctg cgatgtaaaa gaatgaaaaa tgcttttgaa aagagaactg aaaaataaga
     8101 aaagctacac cttatggcag gcttattttt cacaaaatca ttttccaaaa gataatcagt
     8161 gaagaaaggc tcgaaaaaac agttgtac
//
LOCUS       DAWEJW010000113         8132 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2880_length_8132_cov_53.178036, whole genome shotgun sequence.
ACCESSION   DAWEJW010000113 DAWEJW010000000
VERSION     DAWEJW010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2880_length_8132_cov_53.178036"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6067..6166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1938 a         2141 c         1547 g         2406 t
ORIGIN      
        1 tgttaacata tctgtcagac tttgtcaaca actttttttg aaatcttttc aagtttcttt
       61 cgagctgttg tgttgtctcg gcgctacctc aacaacgaat ctgagtatat caccctgtgc
      121 cccacttgtc aacattattt tgcatatttt tcaaacaatt ttgcaaaagc agtaaaaaag
      181 cccataatca tgggcttttt tgtcattttg gatcttattg tcttttctct ttttctcgta
      241 atttacagta tatatgccta taaaagttga attgtatatt catttgatgt atttttgaga
      301 cgaagagaaa tgttttgtat cccatttgcc atatcttcat ttacttccag tagcaggacc
      361 aatccctgtg tctcaccggg agccatggtt tccacatacg tagacatgtc attgggcagc
      421 attgtggtca atgcatttcc acgtgtgccg tcatttacag taatgatgta tctggatgcg
      481 gtactcagga aatcaacatt ttcctcctgg tctgttccgt tggtcagttc aaagttcaat
      541 atcagaagct ttttcccagt ggatgcatcc aacgcaaaat aatcctctgt agatccgtcc
      601 tccggataag agtctgctat ctggtaatct ctgtatgtca aggtcactcc ctccgcaagt
      661 ccgaagaaat cctccagact ggtggtaata tcctcctgtg cggtaggtgc ctgggcttct
      721 gaagcagtcg attccgcagg cttctcttcc gtctgtgcat tctcctgtct tccggtttcc
      781 ttggccgcgt ctttttccag ttttgcaatg acgatttccg ggtccacgag acggctccgg
      841 tgattcgcat catattttaa gagagttacc gccgcatatt ctccgactct ttgtgattcc
      901 gcttctgtca gatccggaat ttcatttccg caggctgtca tgccaagcgc cattacacac
      961 aataacgctc cgcatacaat cctctttttg tttcctgtca ttcttctatc cttccctgtc
     1021 tgtttttcca ttgtttcctg tatttttctc aagtacgata taaatcccgg caatcgccag
     1081 tgcgatcaca cccactgcaa agaaaaggac cgttttctgc aataccggtg ctccccagta
     1141 atcatataat gctattttac cacaagtgca caaagaaagc actagtccat atattcttac
     1201 cggcttatct tttttcaaaa agcccagtgt tacacttacc agcgcaatca gcatgatcag
     1261 aacactattg atcacgggaa cattggtctt aacgatcagt gccatatagg tcagaaagat
     1321 cacaatgatc agttctctgt gtttaaactt cttatagtat ctctcctgca atgtcagtac
     1381 cagataagcc agtacaaaca caaacatgca cagataagta agataagagt tccggtacac
     1441 cggatttgcc agagcgatca acgctccgat ctgccccacc attacaaata cattaaatac
     1501 catgatgttt ttcccgcgca tatatttcac attattaaac agcaatatac ttacaaacag
     1561 gatccccgca aaagccggca gctttagcat cggcatcaaa ttggccgctg caaaaaatgc
     1621 cagggaaaaa gtaagcagga gctcctgtat cgttgtccat ccatagacca ggaacatact
     1681 gaggatggtt cccgcaagga gcaggtatgc atacggtatt tcctttccgt ttgccagaag
     1741 ttcaatacag tacagtgatg taacgatcac atccagtgcc ctcagtccgc cttccgcaaa
     1801 acgggtcagg atcttcgtca gtaaaagcaa cgctgtcagg catatgagta tcggccagtt
     1861 actttctatt acagaaaagg cacataccgc aacaaaactg aaaaagtaac agggcagcca
     1921 ttttttccac gttccacgta tggcaattcc cgcaagaacc gcgactacaa acgccattac
     1981 cgtcagggcg atgattccga tatacgcgtc gttctccgct cttccccaat aattgttcat
     2041 cgccgtaccg gttgtaattc cggctaccag aagaaatgct atatagattg ccagcaagcc
     2101 gttttcattc actgcatggc ccgcctgttt ttcttttagt atgtaattgc tctgcactac
     2161 caggatcaga tgtgcgatca ggaaaaaggt tccaaatgca gctgctgtca cccagaccgg
     2221 cattccctgt gtactgatca cacaggatac cgttaccggg aaaaggatcg tagtgctgac
     2281 aagttgtatc aactgcgaag ccgtatgata tcttcttacc ggcacacaga tgctcagtac
     2341 actcagtaac agaatcaatg ccagcaccag caggccttcc ggctccgaaa gcaatgaaga
     2401 accgaaaagc ggatacgcac aaagccagca tgctgcgatt cccagcattc tgtgtatcag
     2461 ggaatccctt tttctgctca gtaaaaccgt gcccactgtg atcactgcaa tgatcgccgc
     2521 tgcaggaagc ataccgaaat tatgtaggga aatcgtattg atcatggtcg acagatataa
     2581 cgccgctatg ccgatagctg aaaagatccg ggccagtttc ggaagctttc tgtggatcag
     2641 taattctgcg atcaatacca ctcccaggga aactgcatat aaagaaatac ctttgatcca
     2701 gccgttcata aaatacatac caagcaatac cagtgcggtc agaataaaga ttccgccaac
     2761 caccgacaat gcaatggttg cgaccagata ttccgcattc tgcttcttct gtgccggagc
     2821 ctgcgtcacc ggtgcctgca cctgttgcat agtaaactgc cctactggct gtgtctgtga
     2881 ttgcccggct gtctgctgtt ctccggcggc tgcctcctgt ggtacactct gctgcggcac
     2941 agatgactcc gctccggttt ctccgcctct gtttgcattg atctgctgtc tctggtacca
     3001 gaattccacg cttttatcca gttctgcctg tagctgttct acatattccg gctgtctctg
     3061 tattcttgga agtaattgct gtaaatacct ggtaaatatc ggatcattgg actgcatcag
     3121 gtattctctt gcctgttgtt ccagtctctt tgctcgttcc tgttgtgtat tggtctccat
     3181 cttttatcct tccctcattg taatttctgt gatcttatga cactatatac tctctgtatc
     3241 tgtccatatc ctgtctatgg cagtccgcat ggatcagaat accgtcctcc ctatactctc
     3301 tggaaagtat ctgcgcctgt tccatcagat aggaagccac atttcctgca ctgtacggga
     3361 tcagaaatac gcactctgca ttatccgcat agactttctg ttcaacagcc tctgccagtt
     3421 cacggatacc gatattaagt cctgccgcca gataccagtt gtcctgtctc ttttgcggca
     3481 gtggttccat cccacatttg tctaccttat tatacactat gatctgcgga atgtcacctg
     3541 ctcccaactc ttttaaagta tctgctgtta cctgcatctg ctgtctgtaa ttttcatcac
     3601 tgaaatccac cacctggatc agcagatccg catacctgac ctcctccagt gtggaacgga
     3661 atgcttttac cagtccgtga ggcagtttat tgataaaacc tacggtgtct gtcaggaaaa
     3721 acggtttatt atgtccggta tccacactgc ggacactggt ctccagtgta gcaaacagca
     3781 tatccttttc catgaccgtt ttatccggca attcgccgaa ctgctctacc atacggttca
     3841 gaattgtaga ctttcccgca ttggtatatc ctaccagagc cacctgcggg atacgggact
     3901 gctgcctctt tttccgctgc acctctctgg tacgtcccac atcttccagt tcctttttca
     3961 gttctgtgat acgatgatcg atcttacgcc tgtccagttc cagctttttc tcaccggtgc
     4021 ccttattact catgctgcca ctggcaccgc cctgtctgct cagtgcatca tacattccta
     4081 ccagtctcgg cagcatatat tgcagtctgg ctgtctccac ttgtaatttt gcctctcttg
     4141 tctgggcccg gatggcaaat atatccagga tcagattagt ccggtcgatg atcggcagtt
     4201 ccagttcatt tcccagattt cttacctgtg aaggtgtcag agaattatca aacacaatct
     4261 cctgtgcccc gcattcttct gccagcctgc gcacctctgt cacttttccg gtgccgatat
     4321 ataacgcttt attgacatga tccagccgtt gtacaatctg accggccact tctttttccg
     4381 cagcttccgc cagacttgcc agttcctcca tggaacgctc aaaatccggt tcttcaccga
     4441 catccacacc gactaacagt acctttaccg gtgcctcctg tatctgttct tccctcatat
     4501 aaaaatatat cctttatatt tactattttg tctctaactc aaaccagaat tccacgccgt
     4561 tgttgtaatt gttcacaccg aattgctggt tcatggattc catgatggcc ttcacgatag
     4621 acaatcccac accgctgcct ccgtattctc tggttctggc cttatccacc ttgtagaact
     4681 tctcccagat atggtgaata gagtcctccg ggatcggttt gcctgtattg aatacagata
     4741 cccgtacttt gccgtccttt tgttccattt tcacttcgat gatcttatca tcttcacaat
     4801 gattgaccgc attggagaaa aagttcatga acacttcttc tattttaaat tcatccgccc
     4861 atgcatagat cggcggatac tcttccatcc gcacagtgat ctcatgctgt tttgtcagaa
     4921 ttgccgctga ctggatataa tttttcacca gcgccgtaag gtcgaaccgc tccatggcca
     4981 ccacatcatt accaaattcc agctggttca gggtcagaag ctttttcacc atgttgttca
     5041 tcttcgctgc ttcgtccacg atgacatcac aatagaactg cctgctctcg ggatcgtcat
     5101 tgatctcctc ctgcagccct tccgcataac cctgaatcag cgcaatgggg gtcttgagtt
     5161 cgtgggatac attggccaga aattccttgc gcatttcgtc aatggcttct tttttctcca
     5221 cgtcccgttg caattcattg ttcgctgtct tcagttccga aatggttttc tccagggaat
     5281 cggacaactt attgatattc tctcctagca gatcgatctc attatgtgcc ttcccctgat
     5341 attttgcctc aaaatccagc tgtaccatcc gttccgaaat attattcagt tccaggatcg
     5401 gcttggtcac acttcgggag acgaaccaga taataatacc acccgccatt gctcccagac
     5461 atcctgcaag ggcatagaag cggttggcga tctttgcact ctcctgaata ctggaaagag
     5521 gtgtctgcat aataaaagaa atgccggtat tcagtcttcc gtagatttcc agatattcct
     5581 tgtcctcctg tccggttctc tggatcacat agtcatcgcc gttctcgatc accctctggt
     5641 cattgaaagg tatgctgagt ccaaatacat aaccgatcag gcggttttcc agtctctcac
     5701 cgccgttgat ggacacatat ttcatattgg aattcacatc cataacacat accgtcatgt
     5761 tatagtttct gcatacgtca tccagttctc tggcgaattc ttccgagccg tagctgtcac
     5821 tctccgccgc ctgcttgatg gttccgtaag catcatgaat aacatccagt ttgctctgca
     5881 tatagacttt tcccagaaat aaatagttga tcatcaggca cagcaggatc gtaccgatca
     5941 tcaggccgat aaagatccag gcaaactgtc ccctgataga atgtactttt ctcttttgtt
     6001 gtttattccg ctgtttcatc catcttgtac cccatgcccc atatggtctt tatgagattg
     6061 ccttccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatca tcacatccag
     6181 aatgatcaat gcgatatctt tttctttata aaaaatatcc agtgcctcct caccatcacc
     6241 ggcttccagt acatcaaaat tgctcttcac caggaaatcc cggaccagct tgcgcattct
     6301 gctctcatca tccaccacca gtactttcag tctgtccata caccctcacc ttcctatctt
     6361 gagatagtaa ctattcagtc ccattcgcaa aatcgcctct tcgaggcttt cctgaatact
     6421 tacactatag cgtgtaaata tgtcaatttt gtgaaacttc ggtctctata tgtaattctc
     6481 tttccttttc ccgtaccgtc tgctccctct gggtcagttc ctgctcccag gtggcatatt
     6541 tgttctgcag atttctttca taattcagga cattgggctg atcggaattc agcgtgatga
     6601 taaacattgc aataattgca atgaccatca caatattcat aataagagac agccggaacg
     6661 tcgtattttt attttcttcc ggtgccggtt tgattcttct tctggcagga gacgtttttt
     6721 ccctggtgtc cgcaaagttc gtccacagag ggataggcgg aatctcttct cccaacagat
     6781 cctgctctgc tttcagttct ccctgcaatt ccctaaggta ttccaaacct accggagtgc
     6841 ggaacaccct gtcatccaat gcttttttat aaataaccag tacggactcc agatttgtcc
     6901 gatcgatccg cttatccaga taagcaatct tctgtctctc ctgctctgcc agtctggcat
     6961 ctttctctga atagaatgca aatccggaca ctacatgatc cgtccggttt tgctgttcct
     7021 gttctttctg tacatccatt actggttttc ctccgcgctt cctgctgcag cattctgcaa
     7081 taaaatatgt tctccctgta ccagcgggta atggacagga cgatcactgt ccagattctc
     7141 ccggtacata tccactgcag taatctgatg attttcatag gtcgtataat agtaaattcc
     7201 tctgtctgca ttgcagcagg aagtgtagat agtgatctca tactcccctt tgtccagctg
     7261 acagcatcct ctggtctgat ccacgctccc cagaatatgg aaaaactggc tgacgctctc
     7321 cgactcagac aatccggaca gggaattcat cttcgtgaat gccacccgta caaaacggga
     7381 tgcagaagac agatccccgg gcagtcccat ggctcccatg cctctgctgt acggcttcag
     7441 cttcagatct gcggaaaaac ggtttgtcgg tgattcggaa gacagggaca tataatcgtt
     7501 caaacgaaac atctggattg gaaacggggg attgttcgtc aatactccca ccgggttgtc
     7561 atagatcttc agtccctcct gcacagactc gatcgtaatg gcctcttcct tgtctgccag
     7621 aatccagtgc agtggagaaa ccggatattt ttcattaaag gacgtatcca acagattgat
     7681 atgggataac aatactctgg cttcctgcac attggcacac tgccccagaa tccaggggat
     7741 gaactcaaac tgggcgatgt tgtccttacc tgtctgcact ttgctgtata ctgcatttcc
     7801 cgggaaattc agtcccgcca tgccgagtcc tttctcattc actgcatcat agtacaaagg
     7861 ataatcccct gccatatgag caatgccgat catggcataa tgctccgtca gggcattctg
     7921 atgccgtagc ggaaaaggat aattgcgtgg tgttaccgtt acctcttccc catagttaaa
     7981 ttcataatca aaagttctgc cgaaataaaa atcctgtgat ctataagttg ccgctgtaca
     8041 cataacatat actcctttgt tctgattcaa atagatatag aaactagtct tagtctatag
     8101 catttgcaga atgtttgcaa gtatacgaaa ac
//
LOCUS       DAWEJW010000114         8107 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_2887_length_8107_cov_47.084203, whole genome shotgun sequence.
ACCESSION   DAWEJW010000114 DAWEJW010000000
VERSION     DAWEJW010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_8107_cov_47.084203"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1975 a         2132 c         1569 g         2431 t
ORIGIN      
        1 ccggatgttg cgaagaatat cgccctggga cttttcctga atcccgataa ctccggacaa
       61 gagccgccgc tcgatattgc ctgtgacttt ttccgccgcc tgtattcctg tacccagacc
      121 tccgaaatcc ggaagctggc ggagatcgta ctgctgcagg agaaggacga ggctgcctcc
      181 tctaagggcg gtgccaacat cagcctctta aaatcttacg tggaagccca tctggattgt
      241 gagaccctgt ctttaaaatg gctggcagag aactatctct ttgtcagtgt cggctacctg
      301 agcaaacaat ttgtaaagga ggaaggcatc cggttttccg attacctgaa caaaaagcgg
      361 atggaacaag ccatccgcct tatggtgtac tatcataatg acaatatcaa aactattgcc
      421 aggtctgtgg gattcggcaa taatccacag tatttcagcc aggtatttaa aaaatatacc
      481 ggctatactc ccacagagta tattgagagg gagggtgcct ctgcgtcctc ctgatgccgt
      541 ctccctcatc gtaagggaat tacatgcagt tgtccagaag cttcatcaaa gcatcatgcg
      601 ctatggtaaa tgtctcaaga ccgctgagta ccttcttttc tttgacagca atgctcctgc
      661 cgtccttttc cagtgcatgg aaaccctcaa actcaaacag gtgcggttcc agtgacagga
      721 atccatcaaa tccggaggct gacagacgct tcaggatcac cgccacattt ccgtcaccat
      781 agcctgccgg aactacactc tggtctgact tcagtgcatc ctttacatgg atataggcaa
      841 tataggactt taacatctca taggcttcca gcgtatcctg tccgcactgt acaaagttgg
      901 caaagtcaaa aatagcttta aaatgatccc cgtaaaaggt atccatgagt tttttgcatt
      961 ccggagccat tgctccatag atatcttttt cattttcatg caggagcagt gtatcattcg
     1021 cagaggcata gtccacgaat ttaccgatcc tgtcgaagac ttcgccctca taggctgccg
     1081 gatcactctc ctgcggaaga tagaagctga acatccggat cgtcgggcat tccatcttat
     1141 ggctgatctc cactgccctc ttgaactctt caaaatgggg agtgaaatca tccgtgatca
     1201 gaatcttacc caagggagat cccagggcag aaagccggat tccattatca tccagtgtct
     1261 tcttgatctg ctccactttt tcatccgtgt gatagatcag attttctcca tccactccac
     1321 gcatctccac ataagacatt cccagtcctt tcagtcctgc gatctgagct gtcagttccg
     1381 gtgcgatttc atctgcaaaa cctgtgattt ttactgtcat tcttttgtcc tccttaataa
     1441 gtgcctgtcg tatcaaatac aatatcagtg tcttcctttt ttctggaagt ggcacgtttt
     1501 ttgttcaatt ctcgtaaaaa caattcttca tccaacggca gttccacggt gcggttcagc
     1561 cagctggaca ggtgcatggc attggacagg gtcagccccc ggattccctc cgttccctct
     1621 gccaccaatg gagttcctct taaaatatga cctgcgaacg ctttcaatac accaatatgc
     1681 tgctcgtttt gtccatccgt ctcaatctca attctggaat attccggctt tttaaatcct
     1741 tccttctccg ttttgcagaa atcactcaca ttggtggaca gtttatcaaa aaatagtctg
     1801 ccttcctcgc acacgatttt tcccatctcg aaggtcagct ccagccggtt ggttcctgga
     1861 gcatctgccg tggtagtcac gaagacaccg gtggctccgt tttcaaattc catataagcc
     1921 gtcacatcat cctcgacctc gatatcatgc cattttcctt cgtggcagaa ggctctcacc
     1981 ttcacaggca tgccgcacag ccattgcagc agatccagct ggtgcggaca ctggttcaac
     2041 agcacgccgc caccttctcc gtcccagttt gccttccagc ccgccgcatc ataatagctc
     2101 tgattacgat accagtcggt gatgatccag ttgacacgtc gcagctgccc aagttcgccg
     2161 ctctggatca tttcgtgaag ttttctgtat acacagttgg ttctctgatt taacatgatg
     2221 gcaaaggatc tgtcagacct ttccgctgcc tcattcatct cccgtacctg tttcgtgtag
     2281 actcctgccg gtttttcgct gatcgcatgc agtccatgct ccaatgcata gatgacgaat
     2341 tccggatgca gataatgtgg tgttgccacc agaacggcat cacagcttcc cgaatcaatc
     2401 attttcctgc catcatcaaa taccttgacc gtctccggaa gattgttcct tgcccagtcc
     2461 aggcggctct gccggatatc cgctacggcg gtcagttcca tatccggcac catccctttc
     2521 aacacattgc ctgcatgacc ggttcccata ctcccaatac caataatgcc cagtcttacc
     2581 ttttccattt agacaccttt cctttcattc tctaatgtat ttctttgatg ttcttattgt
     2641 aacttctcta cgattgtaat aatatgtata taaaatacta attttatgca agataacgac
     2701 ctttggagtg cctatgaaaa cccaaatgac tattccctat tttacaattg atgcacccta
     2761 tgaactggct taccgcaaca acggaacctc ttctcctgcc agaccgcata cgcacaacgc
     2821 tctggaaata tatcttacaa tgtccgatct cccggatgtc ctcttagacg acactgtctc
     2881 cagtgtttcg aaaggatctc tgatcatcat tccgcctcac catgtgcatc agctgttcaa
     2941 tcagaagctg accatctatg agcgctacgt cctgaatatt aacagcgact ggctgtatac
     3001 tgtccttgga gccggttccg gactcatgcc ttatgcagat ccggctttct ctcctgcaat
     3061 cctccggctt tctcccaccg ggcttaccgg gctctgcagt cagctggatc actatttgca
     3121 gctgcatcct cagccctccc tttctgccta tgcggatttt ttctctctgc taagtatact
     3181 ggatagccgg atccagcacg gcctacaggc atcttctacc ggacagcgtt ctatttccca
     3241 gccccagaaa aacatcaatg aggtcatggc ctttatcaac cggcatctga cagagccact
     3301 gaccctggaa agcattgctg cagaattcta cctgaacaag gactatctcg ggcgcctgtt
     3361 caaggaacat acccatgcca ccatcgggca ttatatctcc gtgcagcggg ctaacctcgc
     3421 ccagacgatg ctggcagagg gccggaccgt caccgaggta caggaacttc tcggcttctc
     3481 cagctatgcc tatttcttta aatttttcaa aaaaatgacc gggatctctc ccagtcaata
     3541 tcgtaaagat aacactatgc ctctgtcaca ttctccgtct ccacattcca aaaaaggaac
     3601 tgcagcctgt gtataaacac acattgcagt tccttttgct tttacctgtc tacttcacaa
     3661 gctatcttat tatttagtca gcagcatcat aatgtcattg cttcttgcca cgaacttggt
     3721 catggcatcg gcagacagag gtgcgtgctg aagctttgcc agatcgtaca gctgctcgca
     3781 gatcatttct gcattctcac catcctggtg ctcagtaatg tactgtacca gaggatggct
     3841 ggcattcagg atcagggtct ctccctcacc gccgaaggca ctcatgtcca taccgggcat
     3901 ggaatacatc ttcatcatat cctgcatacg tctgctctct tcggacagag tgatcatgga
     3961 agagatcttc ttgttcttca gcttctctac cttgacagtg atcttgtcgt tcttcagagc
     4021 tttcttcatc agcttgctga tgctctccgc ctgctcttcc atctctttct cggccttctt
     4081 ggaagtcttg gacttcaggg catcggtcac atccgcatca atacgctgga acttgattcc
     4141 ctcgttcttt ttcttccgca gaaagggtgc tcacacaagt tccgttggca atggcggtct
     4201 gtctggactg ttcctctctg tctttccaga gtactctttc atattccgca gactctctgg
     4261 cacattcccg caggatttct ccgtccaata ccctccacat atgctccgaa tctctgtaca
     4321 gatacggcaa ttgcagtaca ttcatctccg gtgcctgttc tgcgatctgc gacaaggaaa
     4381 ctcttgcaaa agcgatcccg ccgtatttga tctgttccag tacttcgctt tccgaaccca
     4441 gttttgcttc actctccacg atgatgtgga tccgtcctcc ggtacgctcc tgcaccagtt
     4501 ctgcaaaacc tcagcgttac ccagcgccgc atccgggtgc cattgattcg ctccgacgtc
     4561 tctgtgaccg atgccgctct cgtccaaagt tcccggcaga tgcatctgcg ctgccgttcc
     4621 atccgtcaat attaccttcc acgttccctc cagtgaaatc cccatcgcaa tccctctgtt
     4681 cttatataat tttttatatt tttcagtcat cacgaaatat agttagcttc attatagact
     4741 tcacctcaaa ttcaatgcaa cggattctcc ttcatgttct atgcaaaaaa gaaccacatt
     4801 accgatattt ctatcagtaa tgcggttctt gaattcttat cagtatgccc acggttcaaa
     4861 tacaaaatgc cgcataaagc ggaaccacct ttttgttgtc ctcaaacgca aagtttttcg
     4921 cggaaagttt gatcgcgtaa gccggcttat aggtatccat atagaccttt aagcttttgg
     4981 ctctggtatt atcggcggat ttcacctcaa tcgggatcag ctgaccatcc cgctggataa
     5041 cgaaatctat ttcagcccca cgctcggatt cccagtagta agtgttataa ccattgatgg
     5101 caagctgtac attgacataa ttttctgtca tgccaccttt aaaatcattc agttcctcca
     5161 ccatatagag aatatcgttt gcagccaaat ccttcttggc gcaaagcagc cccagatccg
     5221 aaacgtaaat cttaaacgca tcaatatccc gataattttc caaaggcttc ttgatctgct
     5281 ctaccctata gacctgtgac acaattcccg acagacagag ccattcaatc gcattttcaa
     5341 attcagaagc acgtccaccc tttttgatca gcttatattg aaagcgggta tttttcttgg
     5401 aaagctgaac cgtaatatta tcataggcaa gtctggtttt cttaatttca ttcagattat
     5461 tgtatttgcc catatcattc agataacttg tcagaatggt atcctgcgta tggcggatca
     5521 ggatataatc ccctgtctgg acaaactgca tcacacactc cggcataccg cctaccacaa
     5581 gatactgacg gtaaagctgc atggctgcat catggagtgc ggatggcatc ggccgattcg
     5641 tttcaaaaca ttttttaatc tgctctacaa gttcatcctc tcccacagca atcataaatt
     5701 cttccatatc catcggatac agcgttttca tatcaacctt accaacggga aatgaaaatt
     5761 ttgctctgtt gacggcaacg cccaacagac tgcctgccac aataatatga taatccgggg
     5821 catcctcaca aaaatatttc aatgacgtta aagccctttc gcaaagctgt acctcgtcaa
     5881 atacaatcaa tgttttttct tttacaatcg tctgacctgc aatatgagat aaaatcggta
     5941 tcaaataatc aggactgatc ttttcctcaa aggtttcatt cagttttgga ttcgtttcaa
     6001 aattgaaata ggctacattt tcataatgaa ttcgcccgaa ctctaaaata gaataagtct
     6061 tccccacctg tcttgccccc tgcaaaataa gaggtttgcg gtgttcattt tctttccatg
     6121 cttccagaaa acccatgatt tttctataca tgatccatcc tccttaaagg tcctgaatat
     6181 agtataatta ttattcatgt aaaattcaat atgtttttgt gctgtttttg cattaaaatg
     6241 catgaatatt tttatgaaat catcattttc cgacatgttt gctatggttt ttacattttt
     6301 acatttttta cgtttttaca tttttttaca aggaatcgcg tttgcagtaa aagcaccccc
     6361 cgccacaggt ttttattcct gtagcggggg gtgctgtaaa atatgggatt caactatcgc
     6421 agatcagtat tccaatacat aggttgctgt ctcggtaatc gggaagcata cctgtccgcc
     6481 tgcattcgaa actgtcttga caatgcgggt tctgccgttg gcatctacct tgtaaaccgt
     6541 cacttctccg tttttcacag gtactgtggt atggaataca acgtttgtac ccaatgcctg
     6601 tttgttggta aagtcgatgg ttctggaatg ctctgtaaca gtatccttat gttcaaagtg
     6661 ggtttccttg taaccggaca acttgctctt tgcaagaaag gttactgcat cggagttgcc
     6721 gatgaataca tgcaggccac gcagagctga ggtggacact gctttcactg ttgctacagg
     6781 aattgtcttg tcaaagccgc tggcatctac atgaagcaga tcctggttgg caccggatac
     6841 attctgctgc ttgtcttttg tctgggtaga gatcactttt gtaatggcat cccatccggt
     6901 gatctgctct cctgcgatat ttgtggtggt cagtaccggt tcaggagctg atgagccgga
     6961 gtcagaggaa tcactgctgg cgtctccggc ggacggtgca gttgtcgggg tcacaggagc
     7021 aggtgccgga gtaggggtta caggagcagg tgccggagta ggggttaccg atggattttc
     7081 agaatcatcc gatgttgtgc ccggagttct caaaacgtaa tagttatact tactcatcca
     7141 gttttgttcc agactttccg gaaccgggtt atagcctaca cctccttttg cggtgttata
     7201 agtaatataa catacggtac tataattata gattcttcct ataataacgg catatgcatc
     7261 attgggatac ggatgttgca caagatattc attcgcaaac ttttttgctt ctgacaaggt
     7321 aaagaaaggc aggtcagcac tttccagttc ttttatggtc gttgctttca cccagtcttt
     7381 acttccttca ggccattttt tgtaccagtg cgcattaatt tccgttcttc cattatatac
     7441 gcttcttaat gatatgtcct ccttattact ttgggggtct tcactctgag gatcttcata
     7501 ccacccagta aatatatagc cctctttcgt aggtatcgga agatctgtaa atactcctgc
     7561 taccgttgtt atagtatctg tcttccactc accgccgtca agatttaatt taacttcctg
     7621 tgaagggaaa aagtaatgat cagctacaga aatatctata gtatcaagag tcttagaacg
     7681 aacttcaatg tccgcaccat aaaggcatat atattttcca cccttttttc ctaatatgta
     7741 atactcgttt ttacttgact taatataact atgagcccat tcagcattaa aaataaaatt
     7801 atcatatgta gtctggctca aattgtccaa aacggctacc tttgtgtcca agtcgtcttt
     7861 attgtcctcc gtcacctgta tccagcccga gttttcaccc tctgctgctt tcgtgcgcat
     7921 ctccggcacc atacctgcta tcaatgtaac aacaagcgcc aggctaagca cctttcctaa
     7981 aaattttgtt tttctcattc tctttcccct ttcttctgta cttccgattt ctataaatct
     8041 gagttgtgtt tattataaca gcatttccag aaaataagcg gcagattgca ccaatcgacc
     8101 caaaaac
//
LOCUS       DAWEJW010000115         7733 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3037_length_7733_cov_67.133629, whole genome shotgun sequence.
ACCESSION   DAWEJW010000115 DAWEJW010000000
VERSION     DAWEJW010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3037_length_7733_cov_67.133629"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3360..3459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1912 a         2079 c         1495 g         2147 t
ORIGIN      
        1 gccattgcct ccgaatactc ctgaatatag gtaactaaat gtgctcccgg cagggaaccc
       61 tcctgatgca gttcaaatgt attatagatc atctttttcc tcattcccga gcattatcat
      121 tgaaaaataa ggcgcagacc atgtagcaat actaccggcc tgcgtcttca tctgccttat
      181 taatattttc ataactattc agaaccccat tcgcaaaacg ctcctatgga gcttcttttt
      241 tgctcatgtg gttacttcgc cctataaata tcataaaata ttcttacgaa tgccagcatt
      301 cgacgaatat ttgtcgatat ttacatagct ctgaatagtt acatattatc ttagtatgct
      361 cttccccagt ataccatctt tttcgcaggt tttccacaac atacgcaggt atctgcgatc
      421 tgttcctgtt caaaaggcat gcagcggctg gtaacagcaa gcttttcctt gatcttgtct
      481 tcacattcct gacatccgca ccacatagcc ttgacgaatc cggacttttc gttgaagatc
      541 tgctcgaact cttccatgtt atgtgccaca taagtatggc tgtcacgatg tgctcttgct
      601 ctctccagca tttccttctg catggtctcc aggatctcgg ctgccttggt ctccagatcc
      661 tccagagcta cctcggtctt ctctctggta tcacggcggc agatcacaca cttgcctgcc
      721 tcgatatcct taggtccgat ctccacacga atcggataac ctctcatctc agcctctgcg
      781 aacttgaagc cgggggtctt gtcactgtca tctaccttga cacggaaatt cttcgccagg
      841 cggtctctca gctcatacgc cttgtcgaga acgccttcct ttttctgcat aacgggtaca
      901 atgcagatct ggatgggtgc cacacgggga gggatcacca gaccttcgtt atcaccgtgt
      961 accatgatca atgctccgat cagacgggtg gtcatacccc aggaggtctg atgtacatat
     1021 tttaaagtat tgtccttgtc tgcgaactgg atgccaaaag ccttggcgaa accgtcaccg
     1081 aagttatggc tggtaccgga ctgcagtgcc ttgccgtcat gcatcagcgc ttctaccgta
     1141 taggttgcaa ctgcacctgc gaatttctcc ttctcggtct tctgtccctt gatcgcgggg
     1201 atagccagaa cctcctccag gaacttcgca tatacattca gcatctgcag agttctctcc
     1261 tgagattctt ctgcggtagc atgtgcggta tgtccttcct gccacaggaa ctctctggaa
     1321 cgcaggaaag gtctggtctc cttctcccaa cgtaccacgg aacaccactg attgtatacc
     1381 ttaggcaggt ctctgtagga atggatgtca ttcttataga aatcacagaa cagggtctct
     1441 gaagtaggtc tgacacagag tctctcagga agctcattca atcctccctg ggtaacccat
     1501 gctacttcgg gtgcgaagcc ttctacatga tccttctcct tgtccaacag ggactcagga
     1561 atgaacatgg gaagatatac attctgtact ccgactgctt tgaatcgtgc atccagctcc
     1621 ttctggatca gctcccagat cgcatagcct gcgggcttaa ttaccataca tccttttacg
     1681 ctggtgtaat cacacagctc tgctttcttc actacatcgg tataccactg ggcgaaatct
     1741 tcttccatgg aagtgatcgc ttctaccatt ttcttgtctg ccattttctc tctccttttc
     1801 tttttctgaa aataaactgc tcatacgatc tacggaaacg aaaaacgccg ggtcatcgct
     1861 cccgaaaaag ggaccgatca ctcggcggta ccaccctgat tgatacaaat atgtatcctg
     1921 ctctgtcacc gttaacgccg gttctacgct gtgcttcgcc gcttccaggt acattgcgtc
     1981 tcgcacagga ctccaaggga ggtttccgat cctctgtcat aaggggttcc cagccgcacg
     2041 tcaccgtgtc cttcccctct ctctgggatg ctttccgatc gtactgatcc ttttcatcgt
     2101 cattgtcgta tgcaagtaat tgtagtgcat gcttccgtga tttgtcaagg tctatctgct
     2161 aataagaaga tacctgccgg agctgcataa aataaaagca gataataacg caccagggat
     2221 atggggccca gaaagaccgt aaggcacagt gccagaacca gcagccagct gttccatatc
     2281 tctgccctcc gccgccggat gccgtagaaa aacaggatca ctgccagcca gaaataggtg
     2341 ccgattgcaa acaacgcacg gatcaccgga attttctggt aataatattc cagcgatatc
     2401 ttacgataaa attctgtcag ccgggaactg atcgtcttca tttccagtac atttcttccc
     2461 tcgaaatcaa agtaataaat atcaccgtcc ggatcatcca caaccgatgt ccccggatac
     2521 caggcctgat acgtattatc cagaaaggct ctcagataac atcccggata ctgccatcct
     2581 tttcggaacc atagtctcag atactcccac ttattttgaa gaagttcttc attattcaca
     2641 taatttttga tccggtctgc cagaaacgga ttatattgtg accactgttc tctgtccatg
     2701 atctgatcca gcagctgcaa ttcctcactg tcaaacgccg cttctccctt atctgtatat
     2761 acccttgcaa tctgctggag cggtacacac agagcctcta ccacggaacc tttttccgca
     2821 tggagcagag tttccattcc cacagacaat agcatggtca gcagaatcgt tcccgcaaac
     2881 agtgaaatga tctttctttt ctgcttttta cagaaaaccg cgcacaacac cagataaaga
     2941 aaaaccgcat aaattacatt tttccgatac agcgctccca cgatcaggct gcacgataat
     3001 gctgcgattt tctctttctt ccgaaaaaat ccctccgtat tctcatacaa ttcatacaac
     3061 aggtgaaatg caataaaaag tgccagagag cataccacat ccttggtcgt actgacggaa
     3121 aacaaggcaa tcgtcggcag aaaagcataa aaacaaaatc cgactcccaa cagccatttt
     3181 ctattcaatc gccgatacca gaaatccaga ctgtaggaaa aggtcgccgc acaaagtatc
     3241 atctggaagc aggagtgaag aaatactgcc ttctccatcg tcccgaagat ccggtatcct
     3301 atcgtgataa cgcctcccat gaccaacgtg ctgagtaacg aatggtgtgt attatacggn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ctctccctgc atgtacaaac
     3481 agccatcgca gcatctgtcc aaaaagtaat gtctctgcta tccaccacaa aatccaacct
     3541 gctataggaa cctgttccag ccaagactgc atcgttctat gtacggaagt atggtattct
     3601 atactgatcc ggcatcccac tgtctgacag gttgccagta atatcgcagg tattcctaca
     3661 gcaaatctgt cagtccagtc caagtctttc ccggatgctg tccgctgcaa tattccagtc
     3721 acccatttct tcatatgcgg catccatctt ctccttttct ctggtggcac gttctgtatc
     3781 gccggtctga agaaatgcct tatacatgga cgccgcctca aaatatctcg ccacaccata
     3841 gcattccttt ttcgttccgg acagcaagcc accgtacacc atatcctcat gataatttac
     3901 tactaaataa gcataatttt cactctgtac attattctcc atccagttgg tcttcgcagg
     3961 acgggaaaag gcgtcattta attgccggaa catatgaatg gttccccaaa acaatatcag
     4021 gcacagggct atgatcacca gattcatcac aagcagcgcg cctctctgct tctgtgcctg
     4081 tttcagctta ctcttcagat ttaccataac ccatcacctc gcctttttct tccaggaata
     4141 agattttttg tgcatttgac atcgtaccga aattctctgc ttcttccggg gtcatgccaa
     4201 aataggtctg caacagtgcc ttctcattct cttccatccg gagaatggct tccttctgct
     4261 cctccggcag ttcccgatat tgttttgccc tataatcatt atccacagga tgcagaatcc
     4321 tttccagctg ctcgtaataa tccgaaatat cttcatacag accgggaagt atggatacat
     4381 tgccggagtt gatagtaact gccttcatca gactttcata aaagtaacga tacgctccgc
     4441 tggcttccat gagcagctga tatttttcat aattattact gctataaggc cggatgtaat
     4501 gcctgtcgca aaaagctgat aaacctatgt aatcctcatc ttccagatat tgttccatct
     4561 gtttcatata gctgctctgc cggatccctg catcaaactg cagcaagcct ctgtggatgc
     4621 tccagctacg tgcagatact acaaacacca ccacacacag gatcaccagc actgtgatga
     4681 ttatcatatg tgacatgatc tccgtatttt tctccgccac ttcatagaca tcacttttgg
     4741 tatcctcaaa gttgctcttg taatgctcca tgtctttgac atgctgtgcg gcttcttcat
     4801 tcggtcttcc gcaatgcgga caatatgcct gctccaatgt cagattgccc ccgcaatatt
     4861 tacatttcat cattgcttta tgatcctttc ctattcgtta tgcttcttaa tgtattcctc
     4921 gcaatcctca tatctgggat aaccgtaatt atttcgcaga ctatcctcga attttttctc
     4981 ttcttctgcg gtaaacgtat aacggtctgc caggctgttc agggttactt ccacataagg
     5041 acggatatac tccctgtctt tctccgtcag aatgacctcg ctgtagtcca gtccccgcat
     5101 catccaataa tcatccagca gcaatgcctg ctgatactca ttcaaagtct ctccactctg
     5161 ctccagtgcc agatattccc tcgcatcccg acagctcatg agccagcgga aaataccact
     5221 gtgttcccat cggtctatgg ttctgttctc ttcgacagcc tgctcataaa aatccatcag
     5281 tgcttccaga tccttctcct gatagagacg gtccagtatc ggaaagttct ctttttccca
     5341 cagataatcc gctctggcat ccctaggttc tatataccgc acccggaaca caattaccgc
     5401 aagtatcgct gccaatgccg ccaataagat cagaagctta atcacaaatt tctgtttctt
     5461 tttcagtttt ttcttcagtt ccttctccgg tacctgctcc aactgctgca tatcctgccg
     5521 catgctttcc aacttcccca gatattccgc ttcggcgccc ttgtaattca gacttccgca
     5581 atagggacac ttcggaaggg tatcctcaaa ttctgcccca caggaactgc agatgatctt
     5641 tttaactgtg gtatctgcca tacaaatcct catatctttc cacaacctgc acactttgga
     5701 ctgccggcac aaagctgctt attgttcaca atcattatta atatacaaca caaaaatgac
     5761 acgtgcaatg cagaatcact ttttgcacac atcttgaggt ttttgtctat ttcctgtaaa
     5821 ataaactcag tcgaaaaacc tgcccgcagt tcaaaattgc gggttgctga aaggaatgac
     5881 gatcgatatg tatgagaacg gttttctgga tccggcctat atcacaccgg aggaacaaca
     5941 gattttagac ggtaatattg ccgttcagaa gaatctctac accagcaaac aggattttgt
     6001 agtagagagt gataaatttt tggaacactc cgaagatgat attactctgc gtcgccacac
     6061 ccgctttgtg gattttccaa agcataaaca tgattacgtg gagatctttt attgtctctc
     6121 cggttctgtc acacatgtca taaatgaaga agagatctgt ctccgccccg gggaacttct
     6181 atttatgaac caacatatcg aacatgcggt aatagcctgc ggccaggaag atctgggcat
     6241 taacatcatc atccgccccg cctatttcca gaatatcctg actttgatca acaaggataa
     6301 tattctggcg gattttatca tcaatgcgct ggaaggtgac agttgtgtcg gtcagtatct
     6361 gtactttacc gtctccgaca atccctgcat ccagaacctc atccacaatg cggtcttcct
     6421 tctgttacag cagcctcaga actggcataa gctctcggaa cacacctttg ccctgctgtt
     6481 catgcatatc ttaagcaatg cggaaaatat tttattagac cagaaagatg agcagagcaa
     6541 tgtcctgatg gtggtggtac gcagatacct ggcggagcat tatgatatcg gaactcttca
     6601 cgaactggct gcacagatcg ggtacacccc ctcttccctg agccgtatga tcaaaaaata
     6661 ttccggcact acctttaagg aaatacaaac acggcagcgt atgcgagtcg ctatgaatca
     6721 gctgtcccac accgcgctgc cggtgtctga gattgccctg tctgtaggct atgagaatca
     6781 gaacttcttc tacaaacagt tcaaaaaaat gtatgatatg acacctaacg aggcgcgttt
     6841 aaaatcgcgc cgataacgtc aggcattcaa gatataacgg ttcgaggctc cttcgtgtgt
     6901 ccctcggatc gttatttttc atataagaac tcttccggaa gggtcacacc gaagtccttt
     6961 gccagatggc gaaaatcatc gggggtaatg gtctgtctct tattcggctg catggacaac
     7021 atcttcacca gaatttctgc ggatttttct acggtatgca tcagaccgaa ggtcaggtcg
     7081 aagtctttgc ctgcggcaaa ggttccatga tgtgcccaga tggccagatc gtatttcttc
     7141 atcagttcgg aggtagctac tgcgatctct ctgccgcccg gtaccatcca gggcactacg
     7201 cccacaccat ccgggaatac tacggggcac tcggttgcca tctcccacag ttctctggtg
     7261 aataccttat cctccagagg aagcacgaag gtcagcgcaa tgatattggt ggtatgtgca
     7321 tgatatacca cacggtagtc gggatcctgc agcttcttca cttccagatt catcaggtgg
     7381 ctggggagct cggaggtagg tctgcccccg tttaccagac cccatacgat acggtaattc
     7441 tcacccttgt cgtcaagttc gatcatgcag atagaatctt ccggcttaat gctgacatta
     7501 cgaaaatatt tgccactgcc ggtcaccagg aaatactctc ctgccagtgc gggaactgtg
     7561 gtgccgatgg gctggaactc cttcggctca aagttctcct ttaccagttc tacctcttcc
     7621 ggcttcacac ggtaagacag gttgccgccg ttacgttcat gccagccttg ctgccagccg
     7681 tcatctgcca ttctgataaa tccctgtaca aattctgcat ctaaaaattt cat
//
LOCUS       DAWEJW010000116         7479 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3164_length_7479_cov_51.863012, whole genome shotgun sequence.
ACCESSION   DAWEJW010000116 DAWEJW010000000
VERSION     DAWEJW010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3164_length_7479_cov_51.863012"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2322 a         1613 c         1846 g         1698 t
ORIGIN      
        1 cccaccgcgg aaggatgttc cgtttgctgt ggacgttcgt ggatttgaaa ctaccctccg
       61 caatgccccg cgccattcat aagattgtca gcaaagaacg ctgacaatct tatgaataga
      121 cggctgtcaa gtgcgtccga taaggcggga cgcacggtat aaaaatagag tgaaccagcc
      181 tcgcttgtgc gcaagcgccc atcgaggtgt tccactctat ttttgcatgg ctcgtagcta
      241 tgctacatat aaatacataa aaatgaaatg acatacgctt ccgcccatga cgaagaggtg
      301 gaacacttcg tgggagccga agaacttatg tttcgcattg aagatcggca gcttcagggc
      361 atagatgaca ccgcccacgg tgtagatgat gccgccggcc aacagccaga gaaaagcggg
      421 cgtggacaga gttttcagca gaggcccgaa taccaggaca catacccagc ccatggcaat
      481 gtagatcaca gaggaaaacc acttgggaca ggtgatccag caggctttta cgatcatgcc
      541 gacagcggca atgccccaga caagagccag cagtgtatat cccagcttac cgcccaacac
      601 aatgagacac accggtgtgt aagaaccagc gattaataca aagatcatca tatgatccag
      661 cttccggaag acccggagac ttttaccggt gaggtccacg gaatgataag tggcgctggc
      721 accgtataac aggatcatgc tgcccatgaa aatggccatg gccaggaaat tctcctgccc
      781 ggactgtaca ccggctttca ctaacagtgg cacagctgcg aacactgcca tcatcatggc
      841 aatgaaatgg gtaatggcac ttcccggctc tcttatggta atctgcataa taactcctcc
      901 ttttcccggc agcagctgcc gtctgcaaat cgaatctatt taagatcatt ttatccgaca
      961 atagtattta ctattccaaa taacaacacg tattctgaaa tgcttttata tgtagttttc
     1021 aaaactacat cttacaatgc aaatactact gcacaaaaaa ggaaaagtca atagtcattc
     1081 agatatttgg gagaaaagta tcaataagga ttggcaggaa gtctgtagat caacagtcct
     1141 tcatcatcaa ccagtaactc cgcattctct accagacggg catttacttc cggatttgtt
     1201 gcaatgtagc cttcagaacc tttatcccat acaatatatt caggcacttt gtccggatag
     1261 agcgtccagt attggaataa gcgctcatcg atggtcggag tgctgatggt ggaatagttg
     1321 cagatcacgt aatcctcctg aagatacata atgctcttat ggctggcaat cagtactttg
     1381 ctgccccggg gaatgaattc gtctaacaga cgtcctcgga tattatactc gtagccatcg
     1441 gaataccggc cgtagattcc tgccatgggg ccctcttccg ctttctgccg tgtgacgaaa
     1501 atggtgtcgt gaccgtaaat attatacaac agaaatcctt tgtgaatgat ggcaagtccc
     1561 acaaacagca ggacaaaaga tttccagaaa acgggagatt ccgatgtttc ccgctctgca
     1621 tagcataaag aaaccaagat accgatagcc atataatcgg cagactcgta gagcattgta
     1681 ttgctggctg taaaagcggc aaggagcaaa aacagagaag taatacttcc aaaccagaac
     1741 aatgccttat aaacaggggc caattcgcca gaagactttt tacagcggaa ataacaataa
     1801 atgccccaca gtgggaacag aagatagacg aacccgggat actcgatatg cttttgcaac
     1861 cgataccata caaaaagctg ttccagaatc agggaaataa tcaacaacag gaagaaacgg
     1921 tagtttttcc ggcatatgaa tcggaaaaaa caccagaata ccaaagcaat caccagagcg
     1981 accagcagat aaggaaaggt ttcgccgagg caggaaaggt tatcttttag tttcccaagc
     2041 agtccgacat cgtgggagcc gtcggtcagc atttctgaca agccatccag aaactgccgg
     2101 aaagacatat gacacaaaaa gtaggccaac caacccagac cgcagacacc acaggtcccg
     2161 agataaatca gaagattttt cagacggttt ccggcagcag ataacagcca gataccgata
     2221 catccgggaa gtaccacgaa aatacaggtg ggataagaaa gcacaagaag ggaagtgctt
     2281 actccggcgg caatcaggaa aaaggcagga cggcgtgcgg aggtaccgcg attcagacta
     2341 aattccagaa agcttaagaa gaccagagta ctgaaccaca gaagcatatt ggaaaaatcg
     2401 ggaacggtac tgttcttggg aatgatattg tagtaaaaaa cagcaataac aaaagcagta
     2461 ttctcagtta caaacttacg gaaagtccgg taggtcagaa tactgatgag caactgtgta
     2521 acgacaccta cgattcgcag aaagagcacg gagtatttta aggtgtgaaa caactgcata
     2581 tacagccata aaaaggcagc agaaaagaat gcggaagtct gatgaggctc ccacatttcc
     2641 agaaacattc tgtctccctg taccagacgg taagccatag atacggcata ctgctcgtca
     2701 atgtcgaatc cgaagaaaat aatcttgacg gtagcaacta tagatgcggc aagcaacaga
     2761 tatttccaca gattatgcag caatgcggtt gcaaaatttt tctcttgtgc ggaataattg
     2821 ctgttcatct taatcctcct caatgatctg gatctccaga tagtcaaaat cgatggactc
     2881 tacaaaactg tcctgggtaa aacgggtctt gttgtgacgt ttaaagtctg caatattcat
     2941 atccagccag atgggcgtac cgagatcaaa ctgatggcag acttcgtcca gagagtgaaa
     3001 aactttatgg gtacgggtat cgtcactgcc gtcctcgatg caggtgtctt tcagcatatg
     3061 attattttta aaaattttgg cccacatacg gaacatagaa aatctcctta cgcaaaaaat
     3121 cagacatatt ggtcgttaat aataaattat aataggaaga ggtttcccct gcaagcaaaa
     3181 aaagtcacac aaccatcaga tccatcttat attgtatatg ggatagcttt cgtccgccga
     3241 atcggcacgt ttgcgaaagc gtactataaa actgcccaat caatcttaaa aaataataaa
     3301 ggttttgtta aaattataca tttttaaaag gaggtatgct ataattatca taaatacaac
     3361 gtacgaatgc agaatttccg taacgaaaaa gcgtctacgg aagtaacaaa tatgaaaaat
     3421 ggtcgcagag ccttctgtga cagaagagat tgagaggtaa tatggagttt aaggtagaaa
     3481 acggggatgg aatcgacagc tggaaggagg tcacgctggt atacagcgcc gcactgcgtc
     3541 agatggagac aaagatgcag attctgaacg acgagtttca gcatgtacac cagtacaatc
     3601 ccatcgaaca catcaaggca cggatcaaga ccccggaaag cattgtgaaa aagctcaaac
     3661 gctacgggca ggaatccacc atagataaca tggtaaaata cataaacgat attgctggca
     3721 tccgcatcat ctgttccttt acatcggaca tttaccgcat tgcggatatg atccgggatc
     3781 agaaggatat tcaggtgatc gcggtgaagg attacattac gtatcccaaa gccagtggct
     3841 ataaaagcta tcatatgatc gtcagtattc cggtctatct gtcggaccgc acggtggata
     3901 cgaaggtgga gatacagatc cggacggttg ccatggactt ctgggccagc ctggagcata
     3961 agatccatta caaattcgag ggcgatgctc cggagagcat taaaaatgaa ctggtggagt
     4021 gtgcgagaat ggtgtctgat ctggatgcga ggatgctgtc tctgaacgaa gagatactag
     4081 ccatcagcca aaagagagaa gaggaaaaac aattaaatag cgtaacatcc aaataatcca
     4141 aaagcttctg ccggtgtaat acaaacgaca gaagcatttt taattcatag gaaatttctt
     4201 cctatgaatt aaaaatgctc cgcagggatc gcactgcggc ggttatgaaa tatgtcgcaa
     4261 aagcgacccg ccgcaggcgg agaatctcgc aagcgagatt cttttttatg gtcaaaacag
     4321 taatgggatg acaggaaaat ccccataaaa tgaggagtgc ccagacacct ttcggcgcct
     4381 gggctattat gaaaaaattt atcttatatg ctttgttatc cggaagtctt atctcttcct
     4441 tgactgtatt taaagtatac caaccaatta tgaacaaatt atgaacatga tgtgaaaact
     4501 tggtgaattt tacgaaaaag tacagaaacc aaaaaagaaa ctgtacaaat tgcataaaaa
     4561 tgcacaaacc atgaaaacac agtattcatg gggttttggt tgtaaccggg gaataaacat
     4621 ggtaaaatag ggacagttga aaagggaaac tgttcacatg agggacgaaa tatgtttgga
     4681 ggaagatgga tggatacttt tatggataaa cttgcacaaa agctgaatgc ccaggaaatg
     4741 atcaaggcga attccgcggc tgatgccgaa gagatggatc agctgaaaag ccagctgcgg
     4801 gaatacgacg agtgcctggc acagatgcag caggtcaata aggaactgaa agcgatcaat
     4861 caggagatgg agaagctgat gtccgagacc attgctccgg aagtgacgaa gctttccgag
     4921 gaaggcgtgg ctgccctgca gaagctgcag caggagaaca cagagaaact ggaagctctg
     4981 gaacagcagt atactgccaa actggaagaa ctgcagcaga gtaccgaggt gttggacgag
     5041 cttcagaagc atgtggatga gaaactgagc actacggaag agaatgtaca taaggaatgc
     5101 gtaaaggtat accgtaatgt acaggcggtt gtggtagaag agaacgagaa gcagaccgaa
     5161 gccatcgcag caaaagtaaa ggaaaacctt ggcggtaaat tgaacggtgt gctgggagta
     5221 tccattgcgg cagtagttct gacggttgcg gatattgttc ttcatgtatt gagcattttg
     5281 cacattttct aaaaaggcag ataaagtatt catttaagaa tgctgaaatt gataagaaag
     5341 taacggaaat gaggttttac ggaaatggga aaacaacttt ggaagccggg aaatatgctg
     5401 tatcccctgc cggttgtcat ggtgagcctg gctgaccggg acggccgccc caatattatc
     5461 acgctggcct gggtaggaac cgtatgtacc aatccgccca tggtgtcgat ctccgtacgc
     5521 cccgagagat attcctatcc gatcctgaag gagaccggag aattcgtgat caatctgacc
     5581 acgaaggagc tggcatttgc cacagattac tgtggcgtga aaagcggacg ggacgtggat
     5641 aaattcaaag aaatgggact gactcccatt ccggcatctg aggtgaaagc accgatgata
     5701 aaagagagtc cggtgaacat tgaatgtaag gtgcgacaga tcctgccgct gggatcccat
     5761 gatatgttcc tggcggatgt ggtggcagta cacgccgatg aaaaatatat ggatgagaag
     5821 cacaaattcc acctggagca ggcggaaccc atcatctatt cccacggttc ctatttcggc
     5881 tgtggcgaat tgctgggaac cttcggatat agtgtaaaga agcgatgagt aacacaggta
     5941 aaaaattact tgacgtttgc cttccgtttc ggtatcataa gtcataaata agaacatggt
     6001 aaattttact acgaagaagt agcatcagac gggagtctga tgcttttttt atgcggtggc
     6061 gggaaaatat ctgccgggta ccgcaatcat gggaaagtcc gggtgaagca tatgacacag
     6121 atcgataaaa acatagcatt caatctgaaa agaatccgca aatccaaaaa tatgagtctg
     6181 gatatgctgg cggagcgtac cggtgtcagc aaaagcatgc tgggacagat cgagagagga
     6241 gaatccaatc ccacggtgtc cactatcggg aagatcgtgg agggattgaa ggtacccttt
     6301 gaacagttga tttataatag gaaagaaatc atggtggtgc cgtctccgga agaggctccg
     6361 gtatggaagg caaaggaagg ggaataccgg atctatgtga tgctgcccta cgatgccgga
     6421 agaaatttcg agatctatca gggcgagctg accagaaatg cccggatgga aagcgaatcc
     6481 cacggggagc agacctggga atacctgacg gtggtatccg gggaggtcga actgattctg
     6541 gaagattcca gttttctggt gaagacaggt ggttctctct attttgcctg tgacaggaaa
     6601 catgtctatc acaatgtggg tgagaccaag gcggtactca acatgatcat taccacgcag
     6661 cgttagagaa aacgcccccg cttgatggca tccttgccat attttgcccg gatattatcc
     6721 agtgcggcat ccagcttctg ctgtttctcg gaacggggag ccggaaaatc aaacaggctg
     6781 agctgaaccg gttcgtcttc cggcaccagc ttggaagtgc ggatccccag aagccttatg
     6841 ggagtggaat cccataattc gtcgaataat gcacaggcat cttcgtacag gagattcacg
     6901 gaagccgtgg gaatgtctat cggcatttgg tgagaaacgt tccggaaatc actgtaccgg
     6961 atctccgtgc agatctgtcc tgccaactgg tgggaggccc gtagacgcct gcccacggat
     7021 tcacacagag acagcaatac tttacaggct tcctctttgg tggtggcatc gctgcttaaa
     7081 gtggtggagt tgccgatccc ttttgccttg gaaggagcca ggactactgt ggaatcgtcg
     7141 atgccgttgg catactgcca cagacaacga ccgtgacttt ttaaatgtgc ttccaggatc
     7201 gcaggatccg tctttgccag atccccgatc gtgagaatac ctaatttgtg gagagtctcc
     7261 gcactggaat gtccgcaggt gaacagggcg gaaacaggca gcggccacat tttctgcgct
     7321 atttcccgct gatacaaggt gtgagtctta tccggtttct caaaatcaga ggccatctta
     7381 gccagaacct ttcgatccga aatgcccaca tttaccgtaa atttaaattt ttcaaaaaca
     7441 gagtgacgaa tgaaagcagc ggcttcctcg ggagaggcg
//
LOCUS       DAWEJW010000117         7293 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3254_length_7293_cov_78.248687, whole genome shotgun sequence.
ACCESSION   DAWEJW010000117 DAWEJW010000000
VERSION     DAWEJW010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_7293_cov_78.248687"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2047 a         1512 c         1678 g         2056 t
ORIGIN      
        1 aaggagaagg tatcatgaga atagcagtaa catatgaaaa tggacagatt ttccaacact
       61 ttggacacac agagacattt aagatttacg atgtagagga aggaaaagtg ctacattcag
      121 aagtagtaga tacgaatgga agtggacatg gtgcattggc aggagttctt aatgcattaa
      181 atgccgatgt tttgatctgc ggcggaatcg gaggaggtgc tcagactgcg ttagcggcag
      241 cgggcattaa gcttttgggc ggagtttcag gagatgcgga taaagcagta gaagctttta
      301 tcaatgatac gcttgattat aatccggatg tgaagtgttc taaccatgag cacaaccatt
      361 gggaaggaca tacgtgtggt gagcatggat gcggaagcca tagctgtcat taagaagaat
      421 aaaaaaatac aacacgaaag gatgcgtcaa ggtaatgcaa gaattatcgt gatacatcct
      481 tttgtatttg gattgattat ttttttctct gcttgtcgaa agtaatggat ggaatgtata
      541 atattgttaa tgatccagag atatttattt tggaaacagc ttaatcaatt tggaggtata
      601 aaatgaaagc acataaatat tggtcaatcg gagcaatagt tacaatggtt ggaacttttt
      661 atacaggata taaagggtta aaagcagcac acaaatactt tgcatttggc tctctgatat
      721 gcatgactat ggcagtctat tctggtcata aaatgatttc aggtaataaa agaacaagaa
      781 aacaggtgga aaatacaaaa gcggaggaat agtaaatgtt agagttagga ataaaagcac
      841 cggattttga attgccggat caggacggcg taatgcataa attaagtgat tatgcaggca
      901 aaaaagtgat tttatatttt tatccaaagg ataacacacc cggatgtacg aaacaggcat
      961 gcggtttttc agagagatat ccgcagtttg cggaaaaggg tgcggtcatt cttggagtca
     1021 gtaaagattc cgtggcttct cataagagat ttgaagaaaa atatggtctg gcatttacac
     1081 ttttagctga tccggaacgt aaggttattg aagcctatga tgtctggaaa gaaaagaaaa
     1141 actatggaaa aatatctatg ggcgtggtaa gaactactta tcttattgat gagcagggaa
     1201 tcattataaa ggcaaatgat aaagtcaagg cggcagatga tcctgaaaat atgcttaagg
     1261 aattggcata atgaagatta ttttatcacc tgcaaaaaag atgattgtag ataccgataa
     1321 cttagcaccg gttgaactgc ctgtctatat tgataagaca gcagaagtat taaactggat
     1381 gaaaagcaaa tccaaagaag agctgaaagc agtctggaaa tgtaacgata agattgccga
     1441 acagaacttt cacagactgg aaactatgga tctgtaccgt ggacttactc cggcagtatt
     1501 atcctatgaa ggaatcgcat ttcagtatat ggcaccagct gtgtttgagg atagacagct
     1561 tgcatatgta caaaagcact tgagaatcct gtctgcgttt tatggggttt tggaaccaat
     1621 ggatggggtg acaccgtacc ggttagaaat gcaggcaaag gtcgggatag gagatgcaaa
     1681 aaatctgtat gattactggg gggagagatt atatcgttca gtgattgatg acagcagggt
     1741 tatcattaat ctggcatcaa aagaatactc aaaatgcata gaaaagtatc tgacacctaa
     1801 ggatacctat attacggtct ccttttgtga gttatcggga aataaactgg taacgaaagg
     1861 gacatatgcc aaaatggccc gaggggaaat ggtccggttc atggcagaaa gagaaattga
     1921 gaatcctgaa gagatacgaa aatttgacag ccttggatat actttcagaa gagatttgtc
     1981 atctgaatcg gaatatgtat ttgaacgaaa atgaacagac gaaaaatatt atccggagca
     2041 caattaaagt atatcgcctt tacgtctatg ctcatagatc atgtcaataa ggctttgatt
     2101 tatcctaatt taaatggagg aatattgctt gtgataagtg atatttttga cgtattagga
     2161 agaatagctt tcccgatttt tatgttttta ttgatagaag gttattttag aaccacaaac
     2221 agatggaaat atttaggaat agaaaaagga tagcgtaacc gcatggcggg aagcgctatc
     2281 ctttttccta tctgattgca atttttcctt caagatatct tttatttaca tactttttct
     2341 gtcggcaatc tctgccatct tcgcagcatt cagtctggta tcaccatgca ctctggaata
     2401 ggacagatat ccattcatcc ggtcgatctt ggtcagattc ttgctgccgc atttcggaca
     2461 cacatccatc tgcagctcct cgtgtccgca gtcatcacag taggacagtg agagatttac
     2521 cccctcatag aagcccatat ccatggcgcg gcggattaag gatttgatcg ccagcgtgtt
     2581 atagccaatc ggatatttca catactggat cttgccgccg ttacttaagt tccagaaacg
     2641 gttctccaga tcctgcttct ggatcggagt gatgtcctcg gagacatggc agtggaagct
     2701 gttgctgaca taaggtctgt cagatacgcc ttcgatgata ccgtactgct ctctaaactg
     2761 tctaatctgc agtccgcaga ggttctcagc aggtgttcca tagatggcat agagattgcc
     2821 atcttcttcc ttgaactcat ccacgcgctt gttgatatgt tccatgacct ccagtgcaaa
     2881 agcaccgtct tccacaagtg atttcccatt gtagagcatc tgcagctcat tcagagcagt
     2941 gatcccgaag ctggcagttg ctgtcttcag gacgggcttt attttgtccg taagcttcag
     3001 atgtccaccg taaaatccac cctcacaata tgctaaagga ttcgtggatg cacgcatctc
     3061 ccctaaataa gcatacgtcc ggatatggag cctacggatc agctccagat agtaatccag
     3121 tacttcgtag aagtctctgc tctcttttct tgcttttgcc agtatcatcg gcagatgcag
     3181 ggataccacg ccaatgttga agcgtcccac aaataccggt tcgtctccat caaacgccgg
     3241 gtgcataccg ccttgttcgt accagggaga cagaaatgct ctgcagccca tgggggagat
     3301 gatcttaccg tatttcttgt acatacttgc gacatagccc tttccggtca ggctcagcca
     3361 gtcgggatac atggtcttgg cagaacatgc aacacctgct tcaaacacgt cttctaaagg
     3421 tttaccgggg ccatggaggt tctcatcgta taaaaatacg atcttcggaa acagcaccgg
     3481 cttcttatgt ccggtttttc cctggccgcc ccttcggact tccagcatgg caacggaagc
     3541 cattctggca aactgtccgg tgccggttcc ggctgtcacg gtaatgaaag gatagtcccc
     3601 acggctgctt gccacggtgt tgaatttata ttcccagccc tggaagccct gctcgaactc
     3661 cttcttcaca tccgcaagag cttctttttc ggcaagatct gcagcgacac ccagccgctc
     3721 atacttagcc agggctctgc ggtaggattt ctcggcataa ggctctaaga tcagatccac
     3781 actggggaca gtaaagccgc cgtactgctg gctggcagca cttaaaacga tatccccgat
     3841 gacatcgaat gcaacatcca gagtcttcgg ctcattgtac cagatattcc ccatctcgaa
     3901 gccgccggag agcacatgcg ccacatcgaa cagacagcag ttcatggtat cccttctggc
     3961 agacatatca tggatgtaga tatacccgtc cctgcatgcc tggatctctt ctgtcgtcag
     4021 gaagaacttc tggtaaagct ctttgtttaa ctgattaaag atcaggctgc gctttgtgga
     4081 gaccagtgcg gaatctgcat tggaattttc cttatcgccc acatacatga tcgcctggct
     4141 ttttttgtag acatcgtcca gcatccggac aaagtcctgc ttatagttcc tataatcgcg
     4201 gtagcttttg gctacgatgg gacgtacctg ctcgagggca ttttccacga tattgtgcat
     4261 gacagagatg ggtacctgtg tggattccag agcatctata tgagaaatga cgcattcaca
     4321 gatctgttcc ttttcttccg gagtgaactt cgtaagtgcg cgataagcac tttttccgat
     4381 agcatcgatc accttctgga catgaaatgc ctcaagactc ccatcctttt tgatcacata
     4441 ggtctctctt tccggagaat aacgttcgtt gtagttttct acgttgttag tattcatagg
     4501 ctcacgcttc ccatagaccg tggaggttac aataagcata cacggcttcc acagtttccc
     4561 cttcgcagag ggcaaattct gctctcggag ccatatccgg ggtcagattc ttatactgca
     4621 caccgcggtc tgtctgcacg gcgatcaggg agatatagtg ctccggggtc atcggatgct
     4681 ccacggagcc gacatgtacg gagacaatcc cgttttttac cgtatattcg ggaagatgct
     4741 tctccactgc ggcatccacg gtgccgggta ccagttcggt catcttttcc ccgcagcaga
     4801 acaggggaac cttatgatct acaaccttta tagcaacatt tttacaatga tcacaaaaat
     4861 aaaatttaat ttccatagct tttctccttt gcctaataaa aaatatttat tttgacactt
     4921 atagtataac ttgtttcgac cggaaatgat atagaccggg atgtagaata tgataatgag
     4981 aattattatt ataattgtta ataattacca agattcacca gaaaatggca gatttcaaag
     5041 tgatttgtaa gtacaaaaag aatattaacg taaattttat ataaattatg gaaaagcttt
     5101 ttgcaaaaaa ttcagataag atatagtgta gagcgataga tgtttttaag acttttatgt
     5161 tttgtttgca ttaatgttca actgctatga ttaagttgtc agttccatcg gaagatcaaa
     5221 aggtacttgg cacgcagaag aactgtaaga ggatgcattt catgctgtat gaagagggag
     5281 cgacagcatc cataaagtca gtgggcgagg accacaggag gtagcaattg agaacacaga
     5341 tcatcaatga tttaatgacc aatatttttt tcaatctgat cgagacagag gacaggattg
     5401 cagcggatga gcggtttgaa ggtttaagca gcaatgatgc cctggagatc tataaggtgc
     5461 tatctgacca gctgatccgc acccatattt atgccgcaca tatcggaagc gggatcagca
     5521 gcagaatcgc aatgtccata ctgtcgtccg ggagtttcct cttatagaag atctttccgt
     5581 ctcacctgaa ggcataacga tattggatac ggtgtatgtg ccgccattaa tgtaaaaggt
     5641 tgctttcgcc ggatttttcg ggtttacttc ggtggcactg gttaacataa tgctggaaat
     5701 aacatccgta tccacacggt acgtatagtc cggggcttcc agctcttcgg aaggcacatc
     5761 tttgtagctt acaatgccga tgcccaggga agagataatg gcatcatccg acacttttcc
     5821 agaggtgctt ccagtccatg cagggtatcc gagatcagat ttttctaaaa acaacgcgag
     5881 tggcaggttc tggtgcgtca gggagggaag cacccttctt aatgcacctc cggataactt
     5941 gttgtaaagg gcagcctccg ttgctgtcat ggcatagttt tttccattgt gcgtgaagta
     6001 ggcaatgggt tcgatcacca atttgtaatc cccattaatc agattctcaa aggggatgcc
     6061 ggttttggat gcgataaatt tagctgcata ctctgagcag atgtagcgtt ttatcgcagc
     6121 aatgctggct ttgttcacag atccgctgat gattcttggt agagcgacgg ctggctttga
     6181 gtaggtgtag gaggcattaa tggaaagcga tgagcccgca gtatattgca gtttggacac
     6241 ttttccaaag tttaacaccg tggaggaaat gttgcagttt gataaatcaa tgggagtagc
     6301 aacaacgctg cctcccgtgg taacgactgt aacccgaaca ccatcctggt tatttcgcca
     6361 gacgtttagt tcggatcctg taccaaaaga tccaccgcct ccatcaatat ttccatcacc
     6421 catagcataa gtagaaatgc ctgtggataa aaatatcgtt agggatagaa gcagtggaaa
     6481 aaagtatttc ctgattgctc tcataagatc tcctttgcaa taaaaaagac atggcgtgct
     6541 taccatgtct tagttgtttt gttattctcc catagtgccg atctgcttgt tgatgtcgcc
     6601 atcactgtca accacatcat aagtagtatt tccggtatca atccaaccaa ataccggatc
     6661 atagacttgt ccgggatggg aactttctga attatcagca ggaacctgca cttctggtgc
     6721 cgttacagga acctcttccg gataggaagg cacttcgtcc ttatttgtgg tatccccgat
     6781 gggctccggt ttctcggtag gtttggtctc ctgggcttcc tcctttgtca cgcttccgga
     6841 tagatctacg ttagatccgt tctcatcttc agagattaca gtctgcgtgt tgtcggatat
     6901 ttcaccggta taaagagatt cctctttgat ggatgcttca ggaataccag aatctaattt
     6961 gtcatcccag gtttctgcca gggtttccac ctcctgagcg gcaggagtga aggactcctc
     7021 cggttcatgg tttaaatacc aggctccgaa aagaagtccc agacataaca cacttattcc
     7081 cccaatggaa agtgcgactt ttttatcaat attcttcttt ttcataggct gctcctttct
     7141 tggtccttaa ttatatatac gtaagtcggg gtacaaatct tacagacttt tagaattttg
     7201 tattgtggga tccggtaagt tctgcgtgtc ttcggattgg agggaaagac tctccaaaca
     7261 ttcggatccg gtatgtaatg tcgaacgatg cga
//
LOCUS       DAWEJW010000118         7245 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3279_length_7245_cov_58.293324, whole genome shotgun sequence.
ACCESSION   DAWEJW010000118 DAWEJW010000000
VERSION     DAWEJW010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7245)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3279_length_7245_cov_58.293324"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1524..1623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1896 a         1555 c         1982 g         1712 t
ORIGIN      
        1 gtttcttata ccctgccaag ggctacggga ttatttcact aactctcaat ttacaaaaca
       61 tttaaaaaca gtattgactt tttccaggca tatggtaata taaacatatg aacagtggtt
      121 catatgaatg aatattaaaa ctaaatgcaa atcattagca actaaacaaa aaatgcgaat
      181 ggagaaagag gaggatatta ccatgaagaa aacttacaag atcgaagtag actgtgcagc
      241 ttgtgctctg aagatggagg atgcagcgaa gaaggtagag ggtgttaccg atgcaaccgt
      301 cagcttcatg acccagaaga tgaaggtgga atttgccgat ggcagcgatg agaaggccgt
      361 tatggagaac gtggtaaaag cctgcaagag agtagaggat gactgcgaga tctatttata
      421 ggatgctctc ggaaaagagg aagctatgac taagaaacag aaaaaagtac tggtccgcat
      481 cattgtggcg gcagtactgt taattatttt gcattttgta cctctggaag ggatctggct
      541 gggactgctg tacctgattc cctatgcggt gatcggctat gatattctca gaaaagccgt
      601 tctgggcatt atgcatggtg aagtgtttga tgagaatttc ctgatggcgg tagctaccgt
      661 aggtgccatg ggactgggag agtattccga aggcgtggcc gttatgctgt tctaccagat
      721 cggtgaactg ttccaggcgg tagctgtggg caagagccgt cagaacatca gcgccctgat
      781 ggatatccgc ccggattatg ccaacattga ggcagaggac ggcagcttag agcaggtaga
      841 tcccgatgat gtagagatcg gaaccgtgat tgtggtacgt cccggtgaaa aggttcctat
      901 cgatggtgtg gtagtggaag gtacatccac actgaatacc agtgccctga ccggtgaaag
      961 cgtccccaga gagatcacag tggatcagga tgtgatcagc ggctgtgtga acctgaccgg
     1021 attacttaag atccgtacca ccaaggaatt tggagaatcc accgtatcca agattctgga
     1081 tctggtagag aattccagta tgaagaagtc caagtctgag aactttatta cgaagttcgc
     1141 aagatattat accccggcag tctgctacag tgctctggca ctggccatcc tgcctcctgt
     1201 gatcaacctg atcatgggca atccggcagc ctggagcacg tggatcattc gtgccctgac
     1261 tttcctggta atcagctgcc cttgtgcact ggtcatttcc attccccttt ctttcttcgg
     1321 aggaatcgga gcagcaagtg cctgtggtat tctggtgaag gggtccaatt atctggaagc
     1381 gctgtctgag accaaatatg tggtatttga caagaccggt accctgacca agggtgtatt
     1441 cgatgtgacg ggtatttacc ccggcaggga tttcacagag gatgaactgc ttgagtatgc
     1501 tgcatatgcg gagagctatt ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnatgctga ccggtgacag caaaacagtt gcagaacatg tggccggtga gttaaaattg
     1681 gatgaggtac acagtgaact gcttcccgca gacaaggtaa gctgcgtgga gaagctgttg
     1741 caggacaaga gcgagaagga aaaactggca ttcgtgggtg acggtatcaa cgatgcaccg
     1801 gtactttccc gtgcggatat cggtatcgcc atgggagccc ttggctcaga tgcggccatt
     1861 gaggcagcgg atgtggttct gatggatgac aatcctgcca agatcgctct ggctatgcgg
     1921 atttcccgga aatgtctgcg catcgtttac gagaacatcg tatttgcgct ggcagttaag
     1981 gccatctgcc tgatcctggg tgcgctgggt atcgccaaca tgtgggttgc tatcttcgca
     2041 gacgtaggag ttatggtact ggctgtgctc aatgccatca gatgcctgcg cattaaaaac
     2101 aattaaccgg atgcaggcaa aatatgcctg cgtcatgaga tcaccggtat tccgggagaa
     2161 caggtggaca tgcagacttt tttttcagaa attacagcac tgtgcaggaa cgaacaattt
     2221 ctgcacagtg tgatggaaaa atatcattat gatccggaag aatatcccaa tctgaagcga
     2281 ctggcaggtg agatgatccc ctgcctggag acagaagcgg gatggcagga cagagaaatc
     2341 caagagactt ttcccgggag ggatcagtct tttttggctg taggcattac tctgggaaaa
     2401 gggatcgatg aactgcagga aaagtatttg cagaacggat ttctgacaga gagttatatg
     2461 atcgaagtac tgtccagtga attattatta gaaagctacc gggcttatac agagtggctg
     2521 gttgtgcatc ggaatctgca tgttgccaga ctgcattttt taggtgggga gatttctgag
     2581 acttcagaac aaaaaataag cagtctgctg cggctggcga accttcccat gcttctgcag
     2641 gagcttgcat tgccggttac ctgtaatgca gcgtattgta tgatcccgaa gaaaagtgtg
     2701 gtattttatg cggagctgac gaaggatcct tttaccaaat gtgccggaat ctgtctgggg
     2761 tgcggcagga gagactgccc gaaccgcatg gaggaaaagg agaatttccc tctgcggttt
     2821 gcggacatga cggaccgtcc gctgtcctat gggtatgcca gaattttttc caaagcggat
     2881 gtaaaatagg cttatagcgt acaaatcggc agaaagtatt gtccgaaata ttaaaattct
     2941 gataagattt atcagacgac ttgtaagtat agaaagaata tggtatgatg caatcacttt
     3001 caaagaggaa agatatcgtg tgtacggtgt ctttcctctt tttatgtata caatgagtca
     3061 aacacggatt tgtgcctgca caggaggctg tatttgacga agatacatca tcaagcgttt
     3121 tttattcggt gatcaagtaa aagatttgag aagagaggtg gcttatggag gtatttttag
     3181 aggtaacggt acagcataaa aaatatcatg cgggatttat ggaaagaatg cgacagcaac
     3241 cggaagtcac atcctgctat caggtagagg gggaatatga tttcctcgta aaagcggatg
     3301 tgacctcgga aaaggaactg gaaaaactgg agcgcggcgt gcgtaagatt aacggtgtct
     3361 gcagtgtgca gccggtctat gcactgcagg agatcatata gtcatacccg gagcagggca
     3421 ttgaaggtct cctctaaatt cagtctgccg tagccccatt cccggttggg gtagttcaga
     3481 ccctgcgtac gcacggcacc gcgtatgaaa taactgcgga tctcccggct ttccgccagc
     3541 cgattattgc cttcaatgac tgaccattgg aaaaactgtg ctacggctcc ggcagtgata
     3601 gcggcagcca tgctgcttcc cgtacggcat ccgtcaatgg tggagatatt gactccggga
     3661 gcggataagt ccggacggat ctgaccggtg cgtccgaagc ctctgccgga ttctgcgtaa
     3721 aaactgttgt tttccgcgtt gtaagtagaa acgctcagaa cctccggagc catcgagggc
     3781 tccgtcagag tcatgtcggg atccggattg agaaaatata ccggagtaga cagaaattct
     3841 gtgataggca gccacatgtg aaaattaccg ttatataaac taccggaagc agagacatga
     3901 aaggtccaga taccgggggt aggatcctgg atgcgtacaa ggatcagttc ctcgccggaa
     3961 ttttcttcca caagagcaga gtcgatagtg actctgcttt tttcataaat aaaggaatag
     4021 gttacacttt gctgcaatcc caggcgcagt tcaggaatat tttccccgcc gggagtgcgg
     4081 atgctcagat taaaagcatc cggcagagca ccccaaagct ccagccagaa tccgatacag
     4141 ccttcgctga cccggatctc cacattccgg gaatcctccc caggtgcctg agcgcttagt
     4201 cttccatggt aatggtgtga ggcgttccct tcatttcccc cgcagacgat ggcggcacgg
     4261 cttctgcgga cagcaagaga agagagatac cgtgacaggg aggaattgcc cgcgtgatct
     4321 ccctggttgg ttcccagacc cagacagatg actaccggac gttcgaagag ccgcacgaag
     4381 gattccgcat atttgatgcc tagcataata tcattttcct gataggcggg aacaccctcg
     4441 ggcaccagat aaaagctgcg cagatattgc ttacattcct tgagcttcac cacgacgatg
     4501 tcggcacccg gtgcagcacc gatataagga ttgccttgtc tgacgacact gccggctgcg
     4561 actccggcga ggatactgcc gtgtccgttt tcgtccctgg agggaacgat ggaataagga
     4621 tcctcggaac gcagcgccag gttaatatcc tctctgcggt attcagaacc atatttaaat
     4681 ccctcaggtg gtatcccggt ctgaaccgtc tgatcccaga tcgccaggac acggctgttg
     4741 ccgttctcgt ccagaaacac aggattttgg taatcaatcc cggtatcgat aaaacacagg
     4801 atcacaccct gccccgtgag acttaggggc ggacgctgta cctgggtaat cccactggcg
     4861 atcaggtctg ccggatcaaa gggagcggca ccgggtggcg tctcaccgga gggagatagc
     4921 tgcatgagcc cataaagctt cggcatattg tgatagggga aaaaatcaat tttcggattg
     4981 cggattccca gattgcccag atacaaaatc aaaaaatctt ctccgatgcg ttcataacaa
     5041 tcattgtcat ctcccagaaa ttccgatgcg gaaaagtctg tgataatgtc ataagtgtcc
     5101 tctgacagga tcatttcttt gcagtccatg ggcggtgctc cgaagaattc atttttataa
     5161 tatatacaac aggtgatctc tccgtgcatt tccgcagaac tgatccgtgg cataagggaa
     5221 aagctgtatc acatagaata ttgtgaatat caggaaaaag aaggtagaaa taccggcagg
     5281 ggatcgtacg atgtgggaac agtctatttt cacaaaagta tgggaattcg gaaaagatgg
     5341 agcggcaatg gtgaagaagc attataagaa cgggttgatg ctgggtgttt ttttgctggc
     5401 aatgtttttc ctcgggagaa gcatgggggc cattgctttc ggactgcatc ttcagcggga
     5461 gatgagcatc cgggaggcgg cggggagtgc attggagaat actcaggcac tgctctccgc
     5521 ggagaactgg ggactgggat tcggctcgga aggaacgcag cccagcggta cggcttcctc
     5581 ggacaaatta aaggaataca atgcctatta tgtgggagat gcggaggaga aaaagatcta
     5641 tctcactttt gactgcggct atgagaatgg taatagttcc gctatattgg atgcattgaa
     5701 aaggcacaat gcaccggcga ccttttttgt ggtaggacat tttctggagt cggcgccgga
     5761 gatggtgaaa cgcatggtgg aggacggaca tacggtggga aaccacacct atcatcatta
     5821 tgacatgtcg aaaatttctg atccggcggt tttccggaaa gagatggatg acgtgaggac
     5881 tctttttcag gagatcaccg gtacggagat ggagatgtac taccggccgc ctcagggaaa
     5941 atacagtaag acgaatctgc agatggcaaa ggatctgggg tattccactt ttttctggag
     6001 cctggcttat gtggactgga acgtggatgc ccagcccagc cacgaggaag ctttttccaa
     6061 actgacaggc aggatccacc ccggagcggt ggtgctgtta cataatacct cgaagaccaa
     6121 tggggagatt ctggatgaac tgctcacgaa atgggaagaa atgggttata ccttcggaaa
     6181 attggaagaa ctggtgcagt agggtttgac ttcccctgca ccatacccta taatagagga
     6241 cagaaaaata tactggagga caaccatgga aaaagaactg gtagttgtca ttgattttgg
     6301 cggacagtac aatcagctgg tcgccagaag agtcagagaa tgcaatgtat attgcgagat
     6361 ctactcttac aggacagact tgaataagat aaaagcaatg aatcccaagg gaatcatcct
     6421 gaccggtggc cccgcaagct gctatgaacc cggtgcggct acctgctccg aggatctgtt
     6481 taacttagga gttcccgtgc tgggactctg ttacggcgca cagctgatga cccatgtgct
     6541 gggtggtaag gtagaacgcg cagccgttcg ggaatatggc aagactgagg tgaaggtaga
     6601 ccgcagttcc aagctgtttt ccgatgtatc cgagaatacg atctgctgga tgagccattt
     6661 cgactatatc tccaagctgg ctcccggctt ccgtgccgtg gccaccaccg ccaactgccc
     6721 cgtggcagca gcagagtgtg tagagcgcgg actgtatgca atccagttcc atccggaagt
     6781 acttcacacc gtggaaggct ccaagatgct gtataatttc gtacgcaaca tctgtggatg
     6841 ctgcggtgac tggaagatgg attccttcgt ggaagagtcc atcaaggcga tccgcgagaa
     6901 agtcggcaat ggtaaagttc tgtgcgcatt gtccggtggt gtggattcct ccgtggcagc
     6961 agtactgtta tccaaggcag tcggcgagca gctgacctgt gtcttcgtag atcacggcct
     7021 gctccgtaag aatgagggtg atgaggtaga agcagtcttc ggtcccaacg gcaattacga
     7081 tctgcacttc atccgtgtga atgcgcagga gcgtttctac agcaagctgg caggtgtatc
     7141 cgagccggag cataagagaa agatcatcgg cgaagagttc atccgtgtat tcgaggaaga
     7201 ggcgaagaag atcggtgccg tggatttcct ggtacaggga accat
//
LOCUS       DAWEJW010000119         7202 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3302_length_7202_cov_47.418217, whole genome shotgun sequence.
ACCESSION   DAWEJW010000119 DAWEJW010000000
VERSION     DAWEJW010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3302_length_7202_cov_47.418217"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1927..2026
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2155 a         1298 c         1657 g         1992 t
ORIGIN      
        1 atgtcatatt tgggtcgatt ggtgtcaaaa acggtcataa atggaaaaaa taaggtacac
       61 tatcttcaga caacagagaa ctgttgtaag aaggaggaaa ctgttatgaa gaaaaaatat
      121 tttgcatgcg caatgggact ggtactggca ttgtcactgg cagcctgtgg tgaggaagag
      181 gtagttgtgg aggacaccaa cgaagaagtg acccaggaag tacagaccca ggaagtacag
      241 acccaggaaa caacagaagc tcccacagag actgtagcag aggcaggaac ggaagcagag
      301 ggcggtcccc tgttacaggt ggcagttatg gaaatcccgg atctgagtgg tacagaatgg
      361 aactttgccg gcggtatgat cgatggtgtt gagatggaag aagaggatct gaacgcaaca
      421 ctggagactt atggcggtac cttacagctt gtatttggtg aagacggtgc agttaccatg
      481 gtacagggcg gcggaaatgc agaaggtact tatgagtatt ccgatgacaa cacagctatc
      541 aagatgacac ttgattttgg tggaacagag gttacctatg ccggattttt gtctgaggtg
      601 ggagatactg taacactgat cgctatgtct gatatgaatg gttatgacgg actttatttt
      661 gtacagtaag atagtttgaa aagatcttaa aaagacgaaa aggggaggga gagttaacga
      721 tggaaattgt gattatcgtg ttgctgcttg cctccctttt tggtgtctgg ctattgtcgg
      781 tgcggcgtaa gctggagaca caggaagaaa atatccggaa tgccatgtac cagactctgg
      841 tacagctctc cgcagagcgg gaggcactgg aaggattggc ggagtttctc ggacagacca
      901 tggatgcgga gactttccgg agaatgaaac attcgctgga gagtgcacag actgccggag
      961 aggccggact gccggaatgg gtggcagaac aacagagaga attattgaga gtacagaaaa
     1021 aaattgcaga gatcttggaa ggcatgccgc ttttgcaggc acaggagact tatcagaagt
     1081 actggaaagc agcaaaaagc tatgaacata tggtggagac cagtggactg atttataacg
     1141 attccgtgga aaactacaat agtatgcttc acagaatacc ggatcgtata gcagcgggaa
     1201 tctgcgggtt tcaccggaaa aagttaattg agatcatgta atccacatac attcttaagg
     1261 agggtaaaac gatgggatta tttacaaaca acaaaaagtt atgtccaatc tgcggaaatc
     1321 cgacaccgag actgctggca tccgcagtgg aagggcagaa tctctgtaag gagtgtgcag
     1381 ccaagatcga tctgccggac ggcgtactga attccatgac gctggatgaa ttccgggaat
     1441 atatcaactg ttatgatgcc aataagcctc tgcgtgacag tttcacggag acttaccgtt
     1501 atgacttcgg ctttttcaag ggcagtctgc tgctggatat ggatcatcag ctgctgcggc
     1561 tgggagttgt tgacacggca tttgcattgg agccctccga cattaagtcc ttccgtattc
     1621 tggaggacgg cgaggtattg tacgaaggag aaaaggggaa cttccggagc tgcaagagtg
     1681 atatcaagga acgtttaaat gagttaaagc ccaggatcga cgaatacaga atgctgcgtc
     1741 atcagtatga gatgatggag gaaatgcgtc gcagtatgga ggacagcaga agagacgata
     1801 acttcagaag agatgatccg gattacagag accgtatgac cgagccggac tttaatattc
     1861 cgaatccggt ggagaaattc gctgtggaga tcacactgga tcatccttac tggaagagtt
     1921 tctacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatat aacacaacat
     2041 aggcggctgg ctcatttgtg gtgaatgata gattgcagat gttgtattaa gaaggtgata
     2101 ctcttttaat tgaaactgta aacatttagc acaaaggaag gagcagcgta tggagttaac
     2161 atatcattgg gaaggcgatg tattgattcc agatttggaa ttggaagatg gaagcaatta
     2221 tcagattggc aaatatggca gaatgcgaca gaggtatttg tttgaaaatc atcatgccat
     2281 gtatacacat ctggttctta cggaaaaatt gtggaaacat ctggaagaag tagatatgga
     2341 atgcaatgac atgatggata tgcttacggt aagaatggct gagcgtgaaa gtgtgacgga
     2401 gcacttgaag tcagtagatc agctcggatg ggtgcggaag atgaataaca tccggagcag
     2461 agcagaggaa gtagtgttgc atgatctgat ttatagataa gaataaagca ggaatcgtca
     2521 tggttcctgc tttgtcttac catatatacg agaatggagc tattggttgt tattatcatt
     2581 ctcattgact gcttcatcta gcagtaatcg agattccagc tcatccgttg aaggcagtaa
     2641 tgttgcaaga ttttctggta agaaattcga taattcgtat gctgaaattg ctattggttt
     2701 gttggaactg ttaacagcat atttggctaa tacattattt ttttctttgc agatgatcaa
     2761 gccaatagtt tttccatcgg aatccatttt gacaatatcg tcaacaatgt ttatataagt
     2821 acctatctga ccaatatcac gagatttgaa ttcttttatt ttgacctcta ctacgacata
     2881 acaatgtagg tttaggtggt agaataacaa atcaatatat tcctctgtgc cttctatagg
     2941 taaagggtat tctcgaccga caaaggcgaa cccttttccc atttcaatta aaagattcat
     3001 aaccttttcg gtcagggcat cttttaattc tttttcgtta tagtcacgat ttaaagcaat
     3061 aaaatcaaaa ttgtaaggat cttttgttat ttcctgagct aaatcaccgg tgtaagccgg
     3121 tagtgttgtt tgaaaattac ttattgcctt gccttggcgt tcatatagat tagtgtcaag
     3181 gaagtttaga agaacagctc ttgaccagtt attctttatt acttcatcaa cgaagaacat
     3241 tgctttttcg ggttcatctt tgcatttatc aacaattaat tttatatgtc cccatggaat
     3301 ggcatatatt tctgccccaa cttgaggcag atttgtagcc tccggtaatt ctgccccaag
     3361 ttggggcaga atttcaccaa ataaattgta gtatcttttc atgtatctca aattagtggt
     3421 tgaaaatcct tttgcacctg ggaacatctt ttttaaatct tcacttaaag tttcaaaaaa
     3481 agcaccgccc catttgcttt cagcctgcat gttaacgata tccttgccga ttgaccagta
     3541 gaacctaagc aactcgtcat taaccttgat agatgctttt atttgtgtat ttttaaatct
     3601 ttttttaaca tgtttcagcc attcgatata atctttatca attctaatca tattgtccat
     3661 agtatttcct ccagatatgt catttattca ttattcatct gttttatttt tcttagccct
     3721 tgtcttatag acattatact gattcttgca tttcggagag cagaactcat ttcccttacg
     3781 gcttgcgata aatgccctgc cacagtgctt acacagcttc atatcgcagt cagaatccgt
     3841 aagcataaaa gaaaagcaca tctggatcat gacgagcagg gaatggaagt tccatacgat
     3901 aaccggggca ttttccgcta atgagatatg gtaagtcggg gatattccgc caaatgcaga
     3961 cataccctga cgatacagtt tttttgtctg ttcatcaaga ctgtcgtagt ccatataata
     4021 taagaaactt gtggtaaagg taaatgccca gtcggaaaat tctttcacca gccagtcata
     4081 gcgttctgca tattctttct gaaaactcat cgtaacagcc tgtggattct ttcccgttgc
     4141 aagtgcgatt gcaatgccat ccttgtcgga taccgaccaa cttgattcta cccctttttt
     4201 gtgaaaatcc atcttatcaa acggataaaa ataatcgaga taactttctg ttgatagaga
     4261 ttcttcttta ataaaatggt tttttggaag atatacagat tcataggtga taaattccgc
     4321 agttgtcgga agtgcagtca taaagcctaa aaatccgtag gaattgatga aacgaagaac
     4381 catacttttc agttcatctt ctggtgcttt atgcatgacc tgcaaaccga tattgacagc
     4441 agccagtaca agtgattctg catctttcat agggttatac atttctggtt tggcatccgc
     4501 agatactaca atatacaaag ttccatcttc ggcagtgcgg tattcataac tgctgtaccg
     4561 tatccatggg gaactggtat gttcaaatag atttttcatg aaaagcctcc aatctgattt
     4621 cactggaatc agagcaatcg tctgaaattc aattcgtata acttctatat aaagaaactg
     4681 taatctatta acaattctat tcgattacac attataggtc aagggttatc attctgaaat
     4741 ttcaacgcaa ttttttttga caaaacatta aaagtatcat attaccctgt tatatatttc
     4801 aggcagcata gtcactgttt ttcagaattc ttttcgtctt tagttcttac cccgttttca
     4861 aaaaaatatt tttcgtccaa tgtaagtgtc caaggcagat gaagcagcct tgtttcgaga
     4921 gcagtcttat atttctttct tatctgcttt atggcagttt cccttacacc acgcacattg
     4981 cggtctgttt ttccattcag ctcagcgtat tggagagtgg agtatgaccg gacaatcaca
     5041 tagtacaaaa gctgtttctg gtgttctttc atattcttta atatcaggca taaatcctga
     5101 ctgcgtacaa gctcatggat gtaatccggc tcatcaaaga tataatcaat aaaatcgccg
     5161 gcaagtatct gttgccacag taccatgtca tagttggcat taccgggcag gacataatca
     5221 gaggcgtgcc attcaagcgg tacttcagag cgtccgattt catggtatcg ctctctgcgt
     5281 tccctgttgt catcaagttt atcccaccac gatataacat ttgcgaattc ttcctcggtt
     5341 cgtgcacttt cttcaagacg gctgattgcc aatgcacgag cttccctgtg cagcatagtt
     5401 ttctcactgg aagaatcgtt gtattccggt attaagtttt gtttgcggaa agcttttagt
     5461 tcctcatatt ttggcaattt aatcagtcct taaaaatttt tttcaaaaat aatttcaaaa
     5521 cacttccgat tttgtagccg tatttctcct attagtgaag agagtaattc tttttatcac
     5581 taaagcaatt acagaaagga gcaaaaaggc tatgaagtat ggataaataa aagcttcggt
     5641 tacacgaaac ggaagcatga aacaattata atttttatca gagagaaagg caaggattga
     5701 ttatggaggc agaaaaacta aataatggca atagaaagtc cggctcatgt gaagctggat
     5761 ttttttatcg caggattggt gggacagttt acaaaatcag ggtattaacg agtgaaaaca
     5821 gtacagatac cttttcggac aagatttccc atattatttc aagagaggtg ctgacagaga
     5881 aagcggatta tgataagata gagctaccac aaatgagtca gttgccggaa gggagttcgg
     5941 catgaacaga accagacaga caactgacaa atcgaaaaac agaataactg ctctttatga
     6001 gcgactctca agggacgatg agcttgcggg agacagcaac agtatagtga accagaagaa
     6061 aatgctggag gactatgcaa agagtaatgg atataccgat ttggtgcatt ttacagatga
     6121 cggatattcc ggtggaaatt ttgacagacc gggatggaaa gaaatgctcc ggcagattga
     6181 ggacggaagc attggcacag tcattgtaaa ggatatgagc cgagtcggca gggattacct
     6241 acaggtagga ttttacacgg aggtattctt tcgggaaaaa ggtgtccatt tcgttgctat
     6301 ctctaatggt gttgacagtg atatcaacac cagcagcgaa ttcgcccctt ttttaaatat
     6361 catgaacgaa tggtatctgc gtgactgcag ccgcaagata aaggcagtat tgcaggcaaa
     6421 gggcagggac gggaaaccaa ttaccaacaa tccaccatac ggatatatca aagatccgga
     6481 agataagaat cactggctca ttgacgagga agctgcggca gttgtccgca ggatattccg
     6541 gatggcagtt gagggaatcg gaccatatca gattgcaagg atactgactg aggaaaaggt
     6601 ggaaaaacct tcatattatc aggcaaagcg gggatatgga tgttacaaga accggtgtga
     6661 tatgtcaaag ccatatgaat gggcaggagt aagtgtcgta agaatgcttg aaaaaccgga
     6721 atatatggga gatactgtga atttccgtac aaagaagctt tcctataagg ataagactgc
     6781 tgtaagaaat gacagtgacg agatagtggt ctttacggat acccatgagg cgatcattga
     6841 cagaaaaaca tggtatatgg tgcaggagct tagaaagacc aaacgcagga taaacacaga
     6901 gggcgagaca aatccgtttg tgggtaagat tttctgtgcg gactgcggtg gaaagatgca
     6961 ttatcgtaat gagggaaagc gtgccggaag aaactggaga ggactgccgg atggcagtgt
     7021 cagaacaact ccagcctgtt ataactgcgg caattataat aacagccatg accagtcagg
     7081 aaaggtctgc tgttcgcaca atatacaggc aaaggtgatt gaccagcttg tgcttgaaac
     7141 cattcagtat gcgtgtaaaa gtgtaaggat ggatgagaga gcatttgtcg aaagcatccg
     7201 ca
//
LOCUS       DAWEJW010000120         6986 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3406_length_6986_cov_66.183235, whole genome shotgun sequence.
ACCESSION   DAWEJW010000120 DAWEJW010000000
VERSION     DAWEJW010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3406_length_6986_cov_66.183235"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2067 a         1616 c         1473 g         1830 t
ORIGIN      
        1 tgtaacgatt tcgagagtgc gcagcacgaa gaaatcgtgt tattatgtaa accagcattg
       61 agcagtacca aaatcgagag atgtcaattc gactgcttgc aggcgaaatt gacagatccg
      121 attttgatag ggcgatagcc cgacagcaag gaaatgcgaa gcattttcga gcttgtacta
      181 catgagcaag aagggcgata gcccggattg cgaatggatg taacgatttc gagagtgcgc
      241 agcacggaga atcgtgttat tgtaagctcg gcagtgagct tatattgcga atattttatt
      301 taaggagtcc catatgaaca caaaagtact cactacttta gaatatacga aaatcatcga
      361 ccttctcaca gagaaggccg actccgagcc cggtaaaaaa ctatgcagag agctggtgcc
      421 ttctaccgac ctttccgcca tcagaacagc ccagcgggag accaaagatg ccttggcccg
      481 tctcttccgt attggaagca cctcttttgg cagcaacaga gatctaggat tttcgatccg
      541 ttctctggaa atcggcagtt ctctgtctat gtccgaactg ctgaagctgg catcttttct
      601 ggacaacgtc aaccgaatca agacctatgg gaaaaaagaa cgtgaagacc ttcccaatga
      661 cagtctggat gcctactttg aaggccttac tcctatgaca cagctggcta acgaaatcaa
      721 ccgctgtatc ctgtcggaag aagagatggc tgatgatgcc agcccaagat taaagagtat
      781 ccgtcgttcc aagctgtcca ccaacgaaaa gatccacagc cagctgacct ctatggtaaa
      841 tggcgcttac cgtacctttt tacaggatgc agtcattacc atgcgtgata accgctactg
      901 tatccccgta aaagccgaat ataaaagcca ggtgagcggt atggtgcatg accagtcctc
      961 caccggttcc acctttttca tcgagccgac tgctgtcgtg aacctgaaca accagttaaa
     1021 agaactggat ctgcaggagc aggaggagat tgaagttatt ctgggtgatc tgagcagtca
     1081 ggctgctgtt catacctcag agcttgctgc ggatcagaag atcatgacca cgctggactt
     1141 catctttgcc aaggcaaaac tcgccatgga acagaatgcc acagagccta ttttcaacac
     1201 tgagcattac atccagatcc gtaagggccg tcatccactg ttggataaga aaaaggctgt
     1261 tcccattgat gtccgtctgg ggaaagattt tgacttgttg gtgatcacag gtcccaatac
     1321 cggtggtaag accgtgtctc tgaagacagt gggacttttt actctgatgg gacaggcggg
     1381 actgcatatt ccagcactgg atcgttccga actgtccatt ttctcggaag tttatgcaga
     1441 tatcggggat gagcagagta ttgagcagag tttgtccaca ttctcttctc atatgaccag
     1501 agttgtccac attttgcagc acgcagatgc ggattccctg tgtttatttg atgaactcgg
     1561 tgctggaaca gatcctacag agggtgccgc tctggcaatt gctattttaa attatctgca
     1621 tgaccgtggc atccgcacta tggctaccac gcattacagt gagctgaaaa tctatgcact
     1681 ttccactaat ttcgtagaaa atgcatgctg tgaatttgat gtggagaccc ttcgccctac
     1741 ctaccgtcta ctcatcggta ttcccggtaa aagtaatgct ttcgccattt cttccaagct
     1801 gggactgtcc gatgagatca tcaatgccgc gaaagaacag atcagcaaag aagacgaaag
     1861 ctttgaagac gtcatcgcag atctggaaca gagccgcgta accattgaaa aagaacagca
     1921 ggagattgca gaatataagg aacggatccg cacgttacag gaacagttac agaagaaaaa
     1981 cgaaaaaatc gatcaggcaa aggataagat tctgcgggat gccaatgaaa aagcaagagc
     2041 catccttcag gaagcaaagg atgtagccga cgaaaccatc cgtgatttca ataaagcagg
     2101 tgcttccgcc gacatcaagg aactagagaa aaagagacag aaggtccgtg acaagatcaa
     2161 cgaaaagaac ggaaaattgg ctcttggtaa taaccaaaag aagccggcca accagaagac
     2221 cgtggatccc aagaaactga aaaaaggaga ttccgtaaag atcatttcca tgaatctgaa
     2281 aggaattgtg aatacactac cggatgccag gggcaacctc ttcgtacaat gtggaattat
     2341 gagaatgcag acgaatatta acgatctcgt accggtaaaa gaagagacca ttacagcacc
     2401 tgccctgcag cgtaccaaca ccggcaagtt gaaaatgtcc aagtccttct ccgtgtcctc
     2461 cgagatcaac cttttgggat gtacggtaga tgaagccatt gcaaaactgg ataaatacct
     2521 ggatgacgct tatctggctc atctccccag tgtgagagtc gtgcacggaa aaggcaccgg
     2581 tgccctgcgc agtgccgtac agtctcactt aaaacgtctg aaatatgtca aagaataccg
     2641 gttgggtgaa tatggtgaag gcgatgccgg tgtaaccatt gttactttta aataataact
     2701 gacaggaggc ctatactaca tggtaagtaa acagaaaatt ctcattgtag atgatgataa
     2761 taatattgcg gaactgatct ccctatatct gacgaaggaa tgttttgata ccatgatcgt
     2821 caatgacgga gaatccgtga tgccggctat ggagaccttt gctccgaatc tgattctgtt
     2881 ggatctgatg cttcccggta ttgatggtta tcaggtgtgc agagaggtca gggcacaata
     2941 ctccgttccc atcatcatgc tctccgcaaa aggtgaagtc ttcgacaaag tattgggact
     3001 ggagctgggt gcggatgatt atatggaaaa accttttgac tccaaggaac tggtggcacg
     3061 tgccaaagca gtcctcagac gttataagtc ctccaatgcc cccacagaaa cttccaacga
     3121 caagtgcgtg gaatatgcgg atctgaccat taatctgacc aattattccg tcatttatat
     3181 gggacatact gtggaaatgc cgccgaaaga actggaactt ctctactttt tagcatcctc
     3241 accgaatcat gtattcacca gagaacagct tctggatcag atctggggct atgaatatat
     3301 cggtgatacc cgtaccgtgg atgtgcacat caaacgtctc cgtgagaaga tcaaggatca
     3361 tgccaactgg aagatcagca ccatctgggg catcggctat aaattcgaag tacgtaatta
     3421 agtcatacca aaagcaggat ttgttatgaa aaaaacactt tatctaaaat ttatacttgc
     3481 atattttatt tttggtgtat ttggtttcat cattgttaca acctttgttc ccaatatgac
     3541 caaagaacac ctgatccggg aaaaggcaga aagtctctat tccgaagcaa ccctgatcgc
     3601 tggaacctat gccggcggat tgtacaccag tgaaacaact ttggagactg ttaaaatgca
     3661 gctggactcc ctggcggtct acctgaattc cgagattcgc atcatcaatc cctccggacg
     3721 actggtactg gataccaata gtcccttaga tgtggagaat gtggtagtca tcgagaattt
     3781 tgattcgacc gtaaccagcg gctcttatta cattgttggt gatttcttcg gtaattttga
     3841 ttcggatgta cttactgtat ttgctcccat tacctccaac tacaaggtaa aaggctatgt
     3901 agtcatccat accgatatga atgatattca gaaatcctgt aacagcctgc tgaatatttc
     3961 ctatatcacc ctggcaatcc tgttcctgct atccctgatc attctgatct ttttcactga
     4021 gatcgtatat attcctctga ggaagatcac tcatgccaca gagcagtatg ctgccggtaa
     4081 tatgcattat gaatttcagg tggatagtga ggatgaaatc ggttatctgg cttcctgtct
     4141 gaattatatg gcaagcgaaa ttgcccgttc cgaggatgat cagaaaaagt ttgtagccaa
     4201 tgtatcacac gatttccgtt cccctctgac ctctataaga ggatatctgg aagccatgct
     4261 ggatggcacg attccaccgg aaatgcacga aaaatacatt actatcgtat tgaatgaaac
     4321 agaacgactg acgaagctga ccaacagcct gctgactctg aataatctga acaccaaagg
     4381 aatgctctta gataaaacag attttgatat caatcaagtg atccgtaata ctgcaagcac
     4441 ctttgaaggc acctgtcata acaaatctat tgctattgag atgatcctga ccggcaatga
     4501 aatgtatgtc catgcagata tgggcaaaat ccagcaggta ctctataatc tcatggacaa
     4561 tgccattaag ttcagccacc atgattctgt gatccacatt gagacctcgg agaagaaaaa
     4621 taaattgttt gtctccgtaa aagacacagg aattggtatc ccgaaagaag atctgaagct
     4681 gatctgggac agattttata agtccgacct atcccgaggc aaggataaaa aaggaaccgg
     4741 gctgggtctt tccattgtaa aagaaattat caatgctcac aatgagcata tcaatgtgat
     4801 cagtactccc ggcgtgggaa gtgaatttat tttctccctg ccgaaatccc atcaggaaga
     4861 cgaagaggat taagccatac gacttaatcc tcttttttca tctatatatc tatatatcca
     4921 tgacacgata taaataactg tttccctgtt ttccgattcg ttccaccgtt cccatttcca
     4981 acagaatatt caatacggtc tgagccagtg agagagggat gtgggcggct tttgcaaaat
     5041 ctttactgtg aaaaggttct tccagctcat agggcacaaa ttgcagataa tcctccggtt
     5101 gtctgatttc cacctcttcg atcagatccg taggaatcct gtcataccgt gtggagccct
     5161 ttttcttatc atggctccag ccgttcaaca gccggtattc ctccatattc accaatacga
     5221 acttaaatcg gatattcgga tccttcagga acattttgat cttatatagt tccttaaaag
     5281 cctgataggt gcagcctttt ttcggtgatt tccgtttttt actcagttct ccgctgtctt
     5341 catcgatcca gatcagccat ttttcataag ggatcggata gacaatagtc acaggatata
     5401 aaggaagaaa ggtctgcaat tttccccgca tccggttaaa ctgtctcgtc tgaatctcta
     5461 tgatctctcc atccctataa atatccgcta cataattctc tatgggaatc tcctgatgat
     5521 cctcgtccgg ctcataatag tccttaaaaa tagcatgaac cgtcttctct gagagagttc
     5581 cgattcccag tctctgacgg tccacgccta ttatttttct cttagcttct tcaaaacgct
     5641 tctgatccat atgatccata ttttttaatc aatctcggca tgcttcgtat acagaatctt
     5701 aagcaggata ggagtggaga tactggatac aatgatcagc agaatgactg ctgtaaagta
     5761 ttccgcaccg atcattccca cagacaatcc cttctgtgct acgatcaggg caacctcgcc
     5821 tctggtcatc ataccgacac cgattttcaa ggcatccggt cctttgaatc tgcagattct
     5881 cgccatcaga ccacaaccga tgatcttgca cagcagagca acaataacaa atccgataga
     5941 gaacagtaca aattctccgt tcaaatggtc aaagctggtc ttaagtccga tacttgcgaa
     6001 gaaaatcggc ccgaagatca tataagaatt gatatccatc ttctcggcta tgtattccga
     6061 atcccgaata gaacacagga taatacctgc cacgtaagca cctgtaatat ccgcaatacc
     6121 gaagaatact tccgctacat aagcgaaaat aaaacagagc gccagaccta aaataggaat
     6181 acgtcttgta tgcggatatt tgttatccag atactggaat accttgaaca atacaaatcc
     6241 gacaccaatt gcaaacagga agaacagaag agtagaaatt actacacttc ctggattgga
     6301 atccggattt ttaaatccga tgacaaaagt aagtacaata ataccaatga catcatcgat
     6361 aatagcggca ctcatgattg ttgtgccgac tttacctttc agttttccca gttctttcag
     6421 agactgcaca gtaatactta cactggtagc cgtcataatg acaccaataa atacagcttc
     6481 atagaatttc tcagagcccc agggtgccac tccgtaaaat cccatgtaca acaatgttcc
     6541 tcctaccaaa ggaacaaata caccaaagca tgcgatcaga aatgccacaa atcccgtctt
     6601 catgagatcc ctcagattcg tctccagtcc ggcggagaac atcagcagga taacaccgat
     6661 ttctgccatc tggatcagaa aatccgattg ttgcaccagt cccagtacac tgggtccgat
     6721 caacagaccg gcaatgatct cacctaccac ctgtggtgcc ttacattttc tcgcaatgat
     6781 accaaacacc ttggcaaata taataatgat tgccagatct ctgaaaatac tataagattc
     6841 catttttcat acccctcttt ttcatttaag ttattacatt ataacttcac gaattcgtgc
     6901 tattgcattt tacatcctgc tttgcaggat attcgttcac ttgtgagcct ataaaaacaa
     6961 atatctgctt cgcagccgct tgcttt
//
LOCUS       DAWEJW010000121         6805 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3496_length_6805_cov_67.596148, whole genome shotgun sequence.
ACCESSION   DAWEJW010000121 DAWEJW010000000
VERSION     DAWEJW010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3496_length_6805_cov_67.596148"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1952 a         1475 c         1915 g         1463 t
ORIGIN      
        1 taaaggcatg gagcaggaaa agtacttttc ggtgggacgc cagagagaag ctgttagaag
       61 ctgggagcgg ctttcgtctg aggaaaagga agtgcgcctg tgagctgatc tggggaacgt
      121 taacagtaat cagtatccgg atccggtaag cacccgttat gtgcatggcg ttttacgcca
      181 gagtgaaggg catcgtccct taagtagagt ggaaccgcgg ataaattcgt ctctaagaga
      241 agagtttgtc tgcggtattt ttttgagacg attcaaatat caatttgcaa aagatgtctg
      301 aatagtcgaa gtatatttcg aggtgagaac atgggcatca tcaaaaattt aaaagaagaa
      361 gtccgtatta tcagggagcg tgacccggcg atccattctt ccatggaggt acttttgtat
      421 cccagtttca aggtcatgat ccattaccga ctggctcaca agctatatgt gagcgggcat
      481 tatttctggg ccagatatat ttcccagcgt ggtgtacgta agacgggcat tgagatccat
      541 cccggcgcgc agatcggcaa gggatttttc attgatcacg gcaacggtgt catcattggt
      601 gagactacca ttatcggcga taatgtgacc ctgtatcagg gagtgacact gggaggaact
      661 ggcaaggagc acggcaagcg tcatccgacc atcggcaaca acgtcatgat cagtgcgggt
      721 gcgaaagtac tgggttcctt taccatcgga gataattcga agatcggtgc cggaagcgtg
      781 gtcttaagtg aagtgccccc cggatccacg gtcgtgggtg taccgggacg cgtagtaaag
      841 cgtaacaacg caagcctccc ccaggagacc atgaaccaga cagatctccc cgatccggtg
      901 cgggaagata tcacggtatt gcagcgtgag aactccgaac tgaccaaccg cgtgttggag
      961 atggaagatg aactgaaaca gctgcgcagg ctggtggtaa tgagcgaaga agcgaaaact
     1021 gataatacag ttaagacagg aaaggataaa tcatgagtat tcaaatctac aacactttaa
     1081 gcaagagaaa agaagaattt aagcccctgg agccgggaaa ggtaaaaatg tatgtctgcg
     1141 gacctaccgt atacaacctg atccacatcg gcaatgcccg ccccatgatc gtatttgata
     1201 cggtgcgccg ttacatggaa tacaagggtt acgaagtaaa ctatgtatcc aacttcacag
     1261 acgtggatga caagatcatc aagaaggcca atgaagaggg tgtggagccc agcgtgatct
     1321 ccgagcgcta tatcgcagag tgcaaaaaag acatggaggg aatgaatatc aagcccgcta
     1381 ccaagaaccc taaggctacc gaggagatcg gcggcatgct ggatatgatc cagactctga
     1441 tcgacaaggg gcatgcgtat cccgcagcgg atggtacggt atatttccgg accagaagct
     1501 tcaaggacta cggcaagttg tcccacaaga acctggacga tctgcagggc ggtaaccgtt
     1561 ccctgttagt ctccggtgaa gatcagaagg aagatcccct ggatttcgta ttgtggaagc
     1621 cgaagaaaga aggcgagcct tactgggatt cctgctggtg tcagggccgt cccggctggc
     1681 acatcgagtg ctctgttatg agtaagcgtt atctgggtga ggagatagat attcatgccg
     1741 gtggcgaaga tctgatcttc cctcatcacg agaatgagat cgcacagtcc gaggcagcca
     1801 atgacaagcc ttttgcaaga tactggatgc acaatgcctt cctgaacatt gacaaccgca
     1861 agatgtccaa gtctctgggc aacttcttta ctgttcgtga gatctccgaa aaatatgatc
     1921 tgcaggtact tcgtttcttc atgctgtccg cacattacag aagcccgctg aatttcagtg
     1981 cagatctgat ggaagcagca aagaacggct acgagagaat cgttaccagc gtggataacc
     2041 tgaagttcct gctggaaaag gcagcagatg ccgaaatgtc cgaagaagag aagccgttag
     2101 ttaccgaggc acagggcttt gagaccaaat tcgatgaagc tatggacgat gatttcaaca
     2161 ctgcggatgc gctggcagct atcttcgagc tggtaaaatt cgtcaactcc aatgcgaaag
     2221 cagacagcag caaggcattt ctggaggctc tgaaacagga aatcgtgact ctgtccgata
     2281 tctgtggact gatcgtagac aagaaagcgg aaatgttgga cagcgatatc gaggcactga
     2341 ttgaagagcg tcaggcagcc agaaaggcga agaatttcgc aagagccgac gagatccgtg
     2401 acgagctgtt agccaaaggc attgtgctgg aagataccag agagggtgta aaatggaaga
     2461 gagcataacc cttcttacga aaataaagga agcatttgcc tgcgaaggac aggatgtccg
     2521 ggcgtattct ccgctgacac tggcctatat cggagacggg atttatgagc tggtgatccg
     2581 cagcgtggtg gtggagagag ctaaccgttc cgccaatgat ctgcataaaa agacgacacg
     2641 ttatgtaaag gcaccggcgc agtctgccat gatcgaggca ctgcttccgg aactgacgga
     2701 ggaagaggaa gcggtgtacc gcagaggacg caacgccaaa tcatacacca ccgcgaaaaa
     2761 cgcttccgtg gcagattacc gcagagctac cggttttgaa gcattgatgg gctatctgta
     2821 cctgacggga cagatcgacc ggatgctgca gttgatcaaa gacggcatcc gtctggcagg
     2881 catggaactg taataaaaat tcacaataga aaggacaaac atgggatatc aggaatttac
     2941 catagaaggc agaaatgccg tgatcgaggc ctatcgtgcg ggcaaaccca ttgacaagtt
     3001 attcattctg gatggctgtc aggatggccc catgatgacc attaaaagag aagcaaaaaa
     3061 gcatgataca ttagtaaaat atgtgaccaa ggagcgtctg gaccagttat ccgagaccgg
     3121 aaagcatcag ggagtcattg cctatgcggc agcttatgaa tacgcggagg tggatgatat
     3181 tctggaaggc gcaagaaaaa aaggggaacc gccttttatt ttcctgttgg ataacattga
     3241 ggatccccac aatctgggag ccatcatccg taccgccaat ctggccggag cccacggtgt
     3301 catcattccg aagaaccgcg cagcaggact gaccgcgacg gtagccagaa cctccgcggg
     3361 agcgctgaac tatacgccgg tagcaaaggt gacgaatctg gccaagacca ttgaagaact
     3421 gaaaaaggaa ggactgtggt tcgtgtgcgc cgatatgggc ggtaccacca tgtatgatct
     3481 gaatctcaaa ggccccattg gtctggtcat cggtaacgag ggcgagggcg taggacgtct
     3541 ggtgaaggaa aaatgcgata tggtggcatc cattcccatg aagggggata tcgattccct
     3601 caatgcttcc gtggcagcag gcgtactggc ttatgagatc gtgcgtcaga gattacaggc
     3661 gtaatatagg agtggaaaaa aaggggaaag catggcagag catttcaaat atgaagagat
     3721 gacggatgaa gaactcatcg accagctgcg ggaaggtgac agacaggtga tggattacat
     3781 ctgcgacaag tataaaaatc tggtgcgaag caaggcaaaa tccatgtata ttctgggcgg
     3841 agacagcgaa gatctgatcc aggaagggat gatcgggctg ttcaaggcag tgcgggatta
     3901 tgactgcggc agagatgcca gcttttatac ctttgcagat ctgtgtatca gcagacagat
     3961 gtacacggca gtgcaggctt ccaagcgcct gaagcactca ccgctcaaca gctatgtatc
     4021 tctgtatgag cgaagcagcg agggcacgga caatgaggag aaaaatctta ttgaggctct
     4081 ggcggccaga acccagatga gtccggagga actgttcctg gacaaggagc gggtggaaga
     4141 tctggagaga gccattgaga cagaactcag cagctttgaa aaacaggtgc tggatttgta
     4201 cctgacggga atgagctata cccagatcgc caaggtgctg ggaagagacg aaaagtctac
     4261 ggacaatgcc ttacagcgtc tgaagagcaa aatccggaga ctggtgtgat gcaaaataat
     4321 gagagaagag aacacattct tgaaaaaatt tttcaggaat gtgtttttta tagtttttta
     4381 atagaatctg cagagttgga atattgactt aaaacccggc ataggagtat actgcaaatg
     4441 ctgaccgacc agccggtcgg ttaataaagc aaatacatat tcataactat tcagaaaggc
     4501 gaaaaggtca tgcacaatac atggaaaaga aattgcagcg tcggaatggc gttgttgatg
     4561 ggaggcagtc tgttgctcgg aggctgcggc agcaatgggg cacaggatgc tgtggagact
     4621 tctgcggtag tcgtggatat gaagcaggcc cagaccggaa aactgacctt gcagaacagt
     4681 tttgtgggaa cggtttctcc ccaggagatg gtctatgtca ttccctttgc ttccggtaca
     4741 gtgacggata cttattttga ggtgggagat caggtcaatg ccggggatgt tttgttcaaa
     4801 atcgatgact ctgcagcaaa actgcagtta gaacaggcac agctcagtgc ggccaatgta
     4861 aggcagcagg cagacagtgc cctgaccacc cagcaggaat ccgccaatat ccagatggat
     4921 agcagcagag tgcaggcaca gtccgggtat gatcaggcac agatcgcgta tgtccaggcg
     4981 aaaaatgcct atgaccagaa ctatgatgat ctttcagatc agatcgacgc atgcaatgca
     5041 aatattacac agctggagaa tgccataaaa gcagcgtcat ccagtgtgag cggcggaaat
     5101 gcgtcaagtg ttgtgcagat gaagacgcag atcgagacac tgaaaggaac gaagaaacag
     5161 ctggagtcca gcagaaattc tctggtggcc ggattacagc aggcggaatc cgcatacaat
     5221 gcagcaaaga gcagcatgaa tattgtggat cgttcccagg cattgtccca gggacaggca
     5281 ctggaggata cgaagaagca gctgtccacc agtgaacagc tggcaaatgt gggcgtggaa
     5341 agtgcggaac tggcattatc ctactacact gtcaaggctc ccatctccgg tacgattcag
     5401 agcaagggag tggaagtcaa cggcattgcc ggaagcagca atcctgcgta caccattgcc
     5461 aatgaaaata tgatgaccgt gacttttcag gtcagcgaag ctgtgaaaaa tacactgact
     5521 atgggacagg aagtgactgt ggagcgagga aatgccagct ataccggtaa cattaccgag
     5581 attggagtgg cagtgaatca gcagaccggc ctgttccaga tcaaggcagc ggtaaatgcc
     5641 aatggcagtg atctgcccag cggtgtatcc gtgaagctga ctaccgatac ctatcatacg
     5701 aacgacagtg cggtgctgat cccttatgat gcagtgtact atgataatac cggttcttat
     5761 gtatacttaa gtgtggacgg caaggccgtg aagacctatg tgaccacaga tctgtttgat
     5821 gatacacaga tttctgttac tgagggaata cagcccggag ataccgtgat caccagctgg
     5881 tctcccaagc ttatgaacgg tgtggaagtc accgcaccgg tgaaggcaga agagtagggg
     5941 gaggcagcag aatgaatttg acaaagcttg ctctgaaaag accggtatcc tgtttcctgg
     6001 tgctcctggc actggcagta ttcggcatca gctccatttt cggattcaag atggaactga
     6061 ctcccgacat tgagatgcct atgctcatcg tcatggctac ttatcccggt gcagacccgg
     6121 agagcgtgga tgaactggtg gcatccgtta ttgaagattc cggttccact ctgagcggtg
     6181 tggattccgt gaattcttat tcctttgaaa attattgcat ggtgttgttt acctatgaat
     6241 acggtgtgga tattgatgaa tgtcacaacg atctccgtgc ggccatggag acggcgaagt
     6301 taagtctgcc ggatgacgtg gatcagccta ccatcattga gatgaacatg aacagtatgg
     6361 atgtcatgac gatctctgcg gtggaaaagg gtgatgtgga cctgttaaaa gtagtcaatg
     6421 aatccgtggt accggaactg gaatctctgt ccagcgtggc acaggtatcc gtcaccggtg
     6481 gttccgagga ttatatcaaa gtagaactca atgagaccct gatgaagcag tacggtctga
     6541 ccatgtccag tgtggcacag atgctgggaa ccgtagactt tacttatcct gcaggcagcg
     6601 tgacccaggg cagtcaggac atcagcgtgg cgacgtccat ggagtacaat acggtacaga
     6661 agctgcggga agtacccctg atgaccacca aaggacaggt catcacctta caggatattg
     6721 caaatgttac cacagcatcc aaggatgctt ccagtatcag ccgttacaac ggacaggaca
     6781 atgtcagcat cggcattaag aacaa
//
LOCUS       DAWEJW010000122         6332 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3789_length_6332_cov_79.016250, whole genome shotgun sequence.
ACCESSION   DAWEJW010000122 DAWEJW010000000
VERSION     DAWEJW010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3789_length_6332_cov_79.016250"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4491..4590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1794 a         1309 c         1847 g         1282 t
ORIGIN      
        1 aacacgccgg agacgacaaa gtagtaaagc aaggtatcag aaacacaata atgaaatgca
       61 taaggagagc aaaaacatgt tagaaaaagt agcagccatc atcagagaac agttaaatca
      121 ggagaatgta gaaatcacag aggcaacccg tttcaaggag gatctggaag tagactccct
      181 ggatctgttt gaactggtaa tggcgtttga agaggaatat ggtgtatcca ttccttccga
      241 ggaattggaa aatatggcaa cagtaggtga tgttatcaga tatattcagg agcatgcgta
      301 agaacggcat atctgcctga atgcaagagt ccgcaggcaa aagccctcga ttttgatacc
      361 tacggattgc ttcgtgctac gcactcccat gtctccgctc cgcttcggtc cgtgctagac
      421 atgtgccact ggcacatagc accctaccca atattcgcat atcgtgggat tatcatccca
      481 cttttatgct catatcgggg cgggtcctaa ggtctttcac ccacttatga gcaagctcat
      541 gtgggcttag accttttgac cctggtatca tcagaatatg taatataacg cagaatgacg
      601 agaaggatca gaaagacaat gaaaacaaag atcacggaac ttctacaaat agaatatccc
      661 gttttccagg gcggtatggc ctgggtggca gaacataagc tggcggcagc ggtatcaaat
      721 gccggaggct taggtatcat cggtgccgct tccgctccgc ctgaggttgt ccgggaggaa
      781 atccgtaaat gcaaagaact gactgacaaa cccttcggcg tgaatatcat gctgctaaat
      841 cccaatgcgg aggatgtagc gaagattgtc gtggaagaag gagtaaaagc ggtaacgact
      901 ggtgctggaa acccgggaaa attcatggaa ctgtggaaga atgccggagt gaaagtcatt
      961 ccggtggtag ccagtgtggc tatggcgaag atgatggagc gtgccggagc ggacgctgtg
     1021 gtggcggaag gcatggaaag cggtggacat atcggttcca ccacgaccat ggcactggtt
     1081 ccgcaggtag tggatgcggt atctatcccg gtgatcgcag ctggaggtat cgccgatggc
     1141 agaggaatgg cggcggcttt tatgctggga gcggaaggta tccagatggg cacccggttc
     1201 gtcgcatcaa aggaatccat cgtccatgaa aattataaga atcagatcat caaggcaaag
     1261 gatattgatt ctgtagtgac gggaatgtct accggacatc cggtgcgttc tttacgtaat
     1321 cagatgacca gggagtatct gaagctggaa aaagagaatg ccgatttcat ggaactggaa
     1381 aagctgacac tgggatctct caggaaggca gtgcaggacg gagataccgt aaacggaaca
     1441 ctgatggcgg gacagattgc aggcttgatc cgcagagaac agacctgccg tgagattatt
     1501 gaagaaacag tacagcaggc gcaggagtgc atagcggcac aaggacagca taaataaaat
     1561 atagcgacag gtaaccgctg acaacagatt tcgaacagcg acaggcggcg tagttatatg
     1621 gaaaacggcg cattataaaa aatgtgctgt aaccgaggag aacaatagca tcacatagta
     1681 attaggagaa gacaaaatga gcaggatcgc atatttattc ccgggacagg gctcccagta
     1741 cataggaatg ggcaaggagt tctatgatac ttacccggaa gcacaggcgg tatggaagct
     1801 ggcagacaag gtgctccaga atctgtattg gcaggataag cgggaagtac aggcggaaag
     1861 cggacatccg tactccatgg aacagctgac ctttgaagaa aatccttata tcaacattac
     1921 ggaatacaca cagatcgcca tgctgaccat ggaagtggca attctgaaag tattggaggc
     1981 aaaaggactg aaagcggaga tggcagcagg cttaagcctg ggagaatatg gggcgctggc
     2041 tgccgctggt gtgatggagc tgccggatct gttccggatc atccggatgc gtggtatcta
     2101 tatgcaggag gcctatccgg aaggcggagc catggcagca atattaggac tggacggaga
     2161 taccgtggca aaaatctgcg gacagacagc aaaagaaacc ggcaaagtcg tctcggtggc
     2221 aaattataac tgcccggggc agatcgtgat caccggagag gcagatgcag tagcagcagc
     2281 atcggaagcg ctgaaagaag caggtgcaaa acgctgtgta cccttaaagg tcagcggacc
     2341 attccattct gctttgctga aaactgcggg agaacagctg gcagaagaat tgaaaagcgt
     2401 caagctgtca accccttgga ttccttacgt gtccaacgta acagcggatt atgtgacaga
     2461 tgcgtcacag gttgcggaac tgctgaaaca acaggtatcc agtcccgttc atttcaccca
     2521 gagcgtagag cggatgatcg cagatggcgt ggacactttc atagagatcg gcccggggaa
     2581 gacactcggc agcttcgtac ggaaaattga cagaaatgtc aaagtataca atatcgagaa
     2641 accggaggat ctggaccggg tgatggagga actggcatgt tgacaggaaa aagtctgaca
     2701 ggacaagttg ctattgtcac aggggcatca cggggcatcg gaaaagctgt ggcacaggca
     2761 ctggcagcag aaggagccac ggtgatcatt aattataacg gctctgcaga agcagcggaa
     2821 gcggtggcgg cggacatccg aagccaaggc ggcatggcgg aggctgtgaa atgcaatgtt
     2881 gcagattttg cagagagtga agcctttgta aaggcagtaa cagagaaata cggcagagtt
     2941 gacatccttg tcaataacgc cgggatcaca cgggacaatc tgatcgtgcg gatgacggaa
     3001 gaagaattca atgcagtcct ggatacgaac ctgaaaggcg catttcacat gatccggcat
     3061 ctgacgaaga cctttatgaa gcagagatat gggaaaatca tcaatatctc ttctgtcagt
     3121 ggcatcgtcg ggaatgcagg gcaggctaat tattccgcct ccaaggcggg actgatcggt
     3181 ctgacgaaaa gtgtagcgag agaactggca tcccggaata tctgtgtgaa tgccgtggca
     3241 cccggattta tccggacaga tatgacagat aagcttccgg aggcggtaca ggataatgcg
     3301 gttggacaga tcccgcttaa gaaaatggga agaccggagg atgtcgcaca ggcggttctg
     3361 ttcctggcag gagatgcctc ggactatatt accggccagg tgctgtgcgt ggacggcggt
     3421 atggcaatgt aaaaaatacc tccggcaaca gtaaagcaga atagaaaata tgtccgctaa
     3481 cagtacagca ggatagaaaa taagtcagga cgcgagggag tatatcacgt atgggtaaaa
     3541 atagaagagt tgtagtaaca ggcatgggag ctatcactcc gattggaaac agcgtagagg
     3601 aattctggaa tggtatcaaa gagggcaaga ccggattcgg tcctatcact tattttgata
     3661 ctgcggatta ccgttgtaag ctggcggcgg aagtgaagga ttttgatccc gcacaatata
     3721 tggacaaaaa atccgcccgc agaatggagc agttctgcca gtttgctgtg gcggctgccg
     3781 gacaggccat ctcggatgct ggtcttacta tggaacagga agatccttat atggtgggct
     3841 gcagtgtggg aagcggcatc ggaagcttac aggccatgga gcgggaatat gaccgcctga
     3901 aggaaaaagg ccccggccgg gtaggcccca tgctggtgcc tctgatgatc tctaatatgg
     3961 cagcaggcaa tgtcagcatt gcctatgggc tgaagggcaa gagcctgaat gtggttactg
     4021 cctgcgcaac cggtacacat tccatcggag aagcataccg tacgatccag tacggtgatg
     4081 cggatgtaat gatagcggga ggaacggaaa gctccattac ccccatcgga attgccggat
     4141 tttcagcact gacagcgctg tcatttagtg aggatccgga acgggcatcc attccttttg
     4201 acaaggagag aaacggcttc gtcatgggag agggttccgc tatcgtggta ctggaagagc
     4261 tggagcatgc aaagaggcgc ggggcgaaga tctatgcgga actgaccggc tacggctgtt
     4321 cttcggatgc ttatcatatt acatcccctg cggaggatgg caacggagca gccaccgcca
     4381 tgctgaatgc cttaaaagac ggcggggtgg caccggaaga gcttacctat attaatgccc
     4441 atggaaccag cacccatcat aatgacctgt tcgagaccag agcgatcaag nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agtctgctgt tgaagaagta catagaataa
     4621 ccgcagcaga tgttcaagac acaggataga aggcagagca tcaacgagga cgattgcaca
     4681 atgcgaggaa gtagttgata cgacagcatt cgcggaacgg aggaacaatg tcagtttata
     4741 atacacagga aatcatggag attctgcccc acagacatcc atttctgctg atcgatacta
     4801 tcgaggagct ggagcccggt gtgcgggcag tgggacgcaa aaatgtgacc tttaacgaac
     4861 cgtattttgc cgggcatttt ccggggaacc cggtgatgcc gggcgtgttg atcatggaag
     4921 cactggcaca gacaggggct gtagcaattc tgtctctgcc ggagcatcgt ggcaagactg
     4981 cttattttgc cgggatcgat aaggcaaaat tcaaacagat ggtgcgcccc ggagacacct
     5041 tggagctgga gaccaccatt atccgcagta agggaccggt gggtgtcggg gaagcgatcg
     5101 ccacggtaaa cggaaaactg gcggcaaagg cagagcttac ctttgcgtta aaataagaat
     5161 agtctgcgga agtgattttt tatgagcggg acagagagat aaaagtgacg cagaataaga
     5221 ggtgcaggta tgggaagtgt agatacagaa acagagatca aatgcccgaa atgtaagaaa
     5281 atgataaaca gagaccgggc agcgaaaaat aaatatgtct gctatgagtg tggttattat
     5341 ttccgggtga agacccacaa ccggatccgc atggtggcag accggaaaag ctttcagccc
     5401 tggttcgagg agatagaaga gagcaatcca ctgaaatatg agggctatga ggaaaagctg
     5461 tcggaggcca gggaaaaatc cggcgtaaaa gaagctgtga ccgtaggtga atgcgagatc
     5521 tacggggaaa aggctgtgat cggcatctgc gaatccaaat ttatgatggg cagtatgggg
     5581 catgtggtcg gcgagaaagt cacccaggcg attgaaaagg cgacagaatt gcggctgcct
     5641 gtatttttgt tctgctgttc cggcggtgcg agaatgcagg agggaatcgt gtcactgatg
     5701 cagatggcaa aaacctccgc agcgatcaaa cgacatggag aagcaggact tctctatgcg
     5761 actattttga cagatcccac cacaggtggt gtgacggcca gttttgccat gctgggagat
     5821 gtgatcatgg cggaaccggg agccttgatc ggcttcgcag gaccgagagt tattaagcag
     5881 actctgggac agcgcctccc tgatggcttc cagacagcgg aatttttgca ggagcatggt
     5941 tttgtggacg gtatcgtccg ccgggagaat ctgaagaaga ccttatattt cctgatcacg
     6001 acgcatcggt gcagtgaggg gaattatgcg gattttaaga aaaattttga cttccatttt
     6061 gaacctacgg agatcgtgaa ggaacggtca atactgacac tccccagaac ggcatgggaa
     6121 aaggtcaaga ccgtccggag agtggacaga ccggcagcaa ccgactatat tccctacatt
     6181 ttcgattatg tagtggaggc tcacggagac cgttattatg gggatgataa agcactggta
     6241 ggtgcagtgg cattcctgga cgggcagccg gtgacagtgc tggcagatgt gaagggcaag
     6301 gattttgcgg agtgtgccag acgcaattac gg
//
LOCUS       DAWEJW010000123         6237 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3843_length_6237_cov_71.419929, whole genome shotgun sequence.
ACCESSION   DAWEJW010000123 DAWEJW010000000
VERSION     DAWEJW010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3843_length_6237_cov_71.419929"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1772 a         1277 c         1718 g         1470 t
ORIGIN      
        1 gggaacggtt gcaggaaagt ctatggtaga acgtttcgga cagattccgg gattatgtct
       61 tgcttccggt tatacggcac ccagagacct gacagatctg actacacttc cttttctcta
      121 tgaggatatg gagcccttta cgaaccggat catctattat gagaccagca gaggatgtcc
      181 ctaccggtgt agctattgtc tgtcctccat agataagaag gtaagactac gtgacataag
      241 tgtcgtaaag agagaacttc agttttttct ggaccaaaac gtgaaacagg tgaagtttat
      301 tgaccgtacc tttaactgtg accacaaaca tgccatggag atctggcggt atatttacga
      361 acatgacaat ggtgtcacta acttccattt cgagatttct gcggacattt tgcgcgaaga
      421 agagatcgca ttgttgaacc gcttccgtcc gggactggca cagctggaga tcggggtgca
      481 gtctacgaat ccggagacaa tacgagctat tcaccgtgtc atggatgtgg acaagctgga
      541 gaaaattgtt gcggccattc acagaggaca gaatattcat cagcatctgg atctgatcgc
      601 cgggctgccc tatgaggatt atgagagttt cggcagatcc ttcgaccggg tctacgggat
      661 gcagccggag cagctgcagc tgggtttcct gaaagtatta aaaggctccg atatgcatgt
      721 gcatgcacag gaatacggaa tccgttatct ggaacagccg ccgtatgaag tgctgtatac
      781 caactggatc agctatggcg agatccggag actgaaacgg atcgaagaaa tggtggagct
      841 gtattacaat agcgggcagt tcacccatac cctgccggtt ctggaaaaag cataccccag
      901 tccctttgcc atgtatgagg cactggcgga ttattatcag gaacagggat attttaccaa
      961 cagccccgcc agagcctacc ggtatcagat cttattggag ttcgcagcga tgaaagatcc
     1021 ggccaatcgt gagatttaca gagaacttct gacttatgat atgtaccttc gggagaatct
     1081 gaagagcagg cctgcgtttg cggcagagat cacggaagag gaaaaacagg atattcgccg
     1141 cttttatcag acagaagagc atgagagaca ttatctttct gcctatgatc agtatgactg
     1201 gaaacagtta agccgaatga cacacttaga actgttccga tatccggaac cccattacgt
     1261 gttgtttgat tatcaggagc ggaatccgct caattatgaa gccaaggtac aagtgatcgg
     1321 gtatccgata ctataagtac aggaggagct cggttcataa gaattgaggt aggcagtcag
     1381 aatatatcag aagtactgtg gaaggataag aaaatagtat gtatgacact tatgtcagta
     1441 tagatttgga gaccacgggc ctgaatccca agagagatcg gatcatagag atcggtgcga
     1501 tccgtgtgga gcagggacag attgcggaag aattttctac gtttgtggat ccggggcgga
     1561 aattggaaga acggatcaca gaactgacag gaatccggga cgaagacctt gcggatgcac
     1621 ctcagctgga tgaggttttc ccccaattat tagaatttat gggagaatta ccgttgctgg
     1681 gacacagaat tttgtttgat tattcttttt tgaaaaaagc ggcagtggac cggaagatta
     1741 cttttgagag atcggcggtg gacactttgc aggtcgcccg gaaatatctg ccggaactgc
     1801 ctcatcgtaa tctggggtat ctatgtcagt attatgagat cccgcatcat gcacaccggg
     1861 ctctggagga tgcgaaagcc acggaccggt tgttccgaaa gctggcagaa ttgttttatc
     1921 aggaggaaac cggtggacag gcatcgacag aatccgtcga aaagaaagca aacaataatc
     1981 tgtttgagcc gcagccatta cattttcagg taaaaagaga tacgcccgcg acgaaaccgc
     2041 aaaaagagcg gttatatcgt ctggcagagc agcataagat aactttggag gtcgatgtgg
     2101 aaaagcttac ccgcagtgat gcgagcaggc ttgcggataa gattcttgca aagtacggac
     2161 gatagaatta cgtaaagcgg aacgcagttc ttccgcacgg aggatcagat cggcaatttt
     2221 gtcatattcc tcccggccgg catagatttt agccagcgca tagtaagtat ggctgacgtc
     2281 ggtgccgatg gagacggcat acgcgagaac cgtttccgca tcttctgtca gaccggattc
     2341 cagaagcaga tcggcccatt gctgcagagt acgtaccagt acggtgtaat tctgatcata
     2401 ctgggacagc ggtgtaatgt tcgcagtgcc gtattccaac ttgaggtcgg tgttggtgaa
     2461 gcctgtgaga tttacgaccg gctgtgtgga tagataggtc aaaagctcct tatattcccg
     2521 ggctttttca tcctccgaca gagcattcat gggaagtttt tccagaggaa tctttacata
     2581 atccagacca tcaaggggtt ttttgcggac agagttggcc tttttttccc ggtcccagaa
     2641 ttcctgctct ttcttctcgg taagggctcg ctgacgacgt gtattctgtg ccacaacaag
     2701 gatggtatac acgaaaacaa taaatatgaa taaacttgcc aaaaacttta aactcatata
     2761 taatatcctt tcagagtaaa agtgattttt gaagcattta tggaggtttt taccatgttt
     2821 aatatgaata ataaaaaagt aaaacagcga atctccgcag ctatcatcgt gctgcttgtg
     2881 attgctatga tcgtacctac tataacatct attttctact aattcaactg cggatggaac
     2941 agtagatgtg cccgaaggaa gggcagagga gtattatgaa taagtatcgt aaggggtttt
     3001 acggcattct gttattgact ttgacaatgc tgttcggaat gacagcgcag gccaagacag
     3061 atgatactat taagacagga atttatgccg gggatgtgga actatccgga atgacggcac
     3121 aggaagcaac agcggttatc gaagaacata tcgaaagtct gaaggatgtg gagatcacac
     3181 tgcttgcggc aaatgatcac gatgtgacca ccacagccgg agatctgggt gtgacctgga
     3241 aaaatcctga gctggtgcag gaagcattgg aactggggac acatggaaat gtcattgagc
     3301 gttacaagat cctgatggat ctgcagcatg aaaattacgt atatcccatt gaactggatt
     3361 ttgacttaca ggcgatcaac gatctgctga ccagatgcac taagtatgat caggaagcca
     3421 tcaacgtctc tctgaagcgg gacggcggta aatttaccgt agtggaaggt cagaccggat
     3481 atgtgttgga tgtggagaaa tctatcgatg cggtatatga ctatctgaca gaagaatgga
     3541 atcatgaggc ctgcagcatt cctttggaga tcgtagtgga tgagccgaag ggaagtgctg
     3601 aggaactggc acaggtaacg gatgtattgg gaagctttac cacatcttat aagacatccg
     3661 gatcctccag aagtgcaaat gtggcgaacg gctgcagtct gatcaacgga acgactctgt
     3721 atccgggaga agaattttct acttataaga cggtatctcc tttttccgtg gcgaacggct
     3781 attacatggc gggttcttat gtcagcggaa aagtggtaga cagtctgggc ggcggtatct
     3841 gccaggtatc cactaccctg tacaatgcag tattacttgc ggagctggaa gtaacggaac
     3901 gttataatca ttccatgatc gtaggctatg tggatccttc cgcagatgca gcgatcgcgg
     3961 agagctccgg caaagatttt aagtttgtaa ataatacaga tgcaccgatc tacatagaag
     4021 gctatacgca tgacaagcag atcacgttta acatctatgg caaggagacc agagctgccg
     4081 gacattcggt acgttatgaa agtgaagtgc tggagacgat cacaccccct gcggatcaga
     4141 tttatgcgga tgccggtcaa cccatcggtt atatcgtaac agagagtgcg catatcggct
     4201 ataaggcaag actgtggaag atcacgatgg aaaacggtgt agaggtcagc agagaacagg
     4261 tcaacagcag tacttataag atggtgcccc gcagtgccac tgtgggaacc gctacctcgg
     4321 atccccaggc gtatgaagag atcatggcag ctatcagtac agcgaatatt gaccatgtga
     4381 aaaatgtggc agcagcgtta aatgcaagag cagcagcggc tgctgggcag acagaaatcg
     4441 tagacgacta aaggtgagac aatgaacagc ggtaaggtac ccgtgaacgt cttgaaacgt
     4501 tccgttctca gacaactgaa gaataaaaga tcggaaatag caaatagcgc aggtctgggg
     4561 gcagactgcg ctatttttga gcctttttcg gaggggcagc tggtgacctg tgttcaggag
     4621 ggcgtagtgg agctttgctg tgagaacgat ctggcggaag cggaaagaga agatccatcc
     4681 gtgatgccta tgcgtcgctt tttccagaaa tgtgccaaca atctggcaac tgccggtgca
     4741 gagcccgtgg cagcggaatt ggtgatcttc ctgccggaga gcgtggaaga accggaattg
     4801 aaagcactga tgtccgaagc ggaaggtatt gccgcagagt taaacataca gattatcggc
     4861 ggacagacca gagtaagttc cgcggtacgg cagcccctgg ccacggtgac cggatatggc
     4921 atccgcaaga ctggtgcggt gcagatggat gtgcggaaga agctggcggg acaggatatt
     4981 atcattacga aatggatcgg cctggaaggc actgcggatc tggcggcccg gaaccaggaa
     5041 gaactcctga cgagataccc ggcgtatctg gtggaagaag ctgcggcgtt tgatcggtat
     5101 tattctattt tgccggaggc cgctaccgcc gtgaagtccg gcggttgtac catgcatgat
     5161 atttccgaag gcggtgtatt tgcagcgctg tgggagatgg cagagggtgc aggtgttgga
     5221 ctgacaatag atatgaagaa acttcctctc agacaggaga cagtggaggt atgtgaattc
     5281 tgtaatgtaa atccgtatga actcagatcc ggcggcagcc tgatcattgc aagcccggag
     5341 ggaaccgcag tggtggaagc actggcggca gagggaattc cggcagtgat cgtaggaaga
     5401 tttacagaca gtaatgaacg actgatcctg aatgaggacg agatccgcta catggatcgt
     5461 cctcagagag atgagatcta taaagaaaca gaataaaagg agatattggc atgagagaag
     5521 aattattagc gatcatagag aagaacagca ggatcgatct gaaggaactg gcagtgatcc
     5581 tgggagtgga agagatcgat gtggtaaatg aactggcagc actggaagca gaagggatca
     5641 tctgcggtta tcataccctg attaactggg agaagacaga cattgaaaaa gtaagcgctc
     5701 tgatcgaggt acgtgtaact ccccagagag gccagggctt tgacagtatt gcagagcgta
     5761 tctacaaata tcctgaagta cgcagtgtgt atctgatgtc cggagcgtat gatctgatgg
     5821 tcattctgga gggtaagacc ctgcgggagg tatctaattt tgtatctgac aagctgtcca
     5881 cactggattc cgtgctgagt acagcaacgc attttatcct gaagaaatac aaggatcacg
     5941 gaaccgcatt tgctgtaaaa aaagaagatg aaagagagat gatcacccca tgagaaatcc
     6001 attagctgat aaagtagtgg atatcaaacc atcgggtatc cgcaaatttt ttgatattgt
     6061 aagtgagatg caggatgcaa tctctctcgg tgtgggagaa ccggacttcg acactccctg
     6121 gcacatccgg gatgagggca tctattccct ggaaaaggga agaacctttt acacctccaa
     6181 tgcaggtctg aaggatctgc gtcaggagat ctgcaacacg atccagagaa aacaggg
//
LOCUS       DAWEJW010000124         6152 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3919_length_6152_cov_57.439232, whole genome shotgun sequence.
ACCESSION   DAWEJW010000124 DAWEJW010000000
VERSION     DAWEJW010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3919_length_6152_cov_57.439232"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1510 a         1612 c         1250 g         1780 t
ORIGIN      
        1 ctccgtaatt tcaccgtcag acattctttt gaactattgt aatctattca gaaatgcctt
       61 tgcattggct gcaatatcat ccttaaatac ggcggaaccg gctacaatga cattggctcc
      121 tttttccatg gcaaatgcta cattgtccag attgatacca ccgtctactt ccagatcgat
      181 agacagcccc ttatcgttga tcattttccg gatcacctgt accttatcca ggcactccgg
      241 gatcagcttc tgtccgccga atccgggctc cacagtcatt accagcacca tatccaccag
      301 ttccagataa ggttccactg cctctgccgg agtggcgggc ttaatggtga tgccggcctt
      361 tttgcctgtg gcatgtattt tttcaatgca tccctccaca tcttctgtgg cttccaggtg
      421 aaaattcacc aggtccgcac cacaggcagc gaactcttcc acataacgtt ccggcttctc
      481 gatcatcaga tgcacatcga gaaacatatc cgtgtgggat ctgacagctt ttaataccgg
      541 cataccgtag gaaatcgacg gtacaaacaa tccgtccatc acgtctacat gcagccaggg
      601 gactcctgct ccttccacag ctttgatatc ctctcccagt ttccataaat ccgccgaaag
      661 aattgaaggt gataataaat tcatgttact cctcatttct gccccgcaag gccttgtctg
      721 ttgcttatta ccgttctatt ctagcaaact tgttttcgta aaacaagact aacgaaaagg
      781 aaccttttcc ttttggaaaa aagttcctcc gttcgcaatt ctctataggt ttttcacgtc
      841 ttctttcagt ttgttccagg catcttcatg ctccgtatag taaatgggac atttctttcc
      901 gccacaatca tagtggcgca agatgtcatc gggttgcagg tcataggcat cagtcagcca
      961 tttcaataat ttgatcagag aatcataggt ctcctgagta aactggccgt tgtccgcctt
     1021 gtagcagcat tcgatagaaa tagaatcctc attgcgggtc tgtactgcat aggcgatctc
     1081 gtccagagga acgcactgaa ggatctcgcc atcatagcca atgataaaat gggcactggc
     1141 actgcgttca tgattgtctt tcagctgttc gaaatagctg cggttctggg ccgcactggt
     1201 tccaggattc gccgtgtaat gtacaaaaat atttttcact tccgtcaggg gatctcccgg
     1261 tctggaatat tcattcacgg tgagaaaatc ttctgtccag ttgggatgtg ctgcatatac
     1321 atccgccgga agtccctgga tccgggttcc cgtggtctcc ccgatctgtt tttcgatccg
     1381 ctgcacagct tctgccgcaa cttccacagg agtactggcc tccgcagtgc tggcaggtac
     1441 ttccaacaaa tttttcaaca taaagattcc tgcaagaatg ctggcacaca gcagggaaat
     1501 cttaatatat ctgcggattc ttgcctgtct gcggcggcgc tgcctctcgg ctctgacccg
     1561 gcgttgtctg acgcgggtgc tgtaagcatt gtctttgtaa acattttgct catatgcatt
     1621 gcccggatga ccctgctcct gccgccaggc cttgtttttc tgctgtgctg tcgtcgaata
     1681 ctgcggatgt gatttcttcc ttccgccttc ctgcggcatt gccgatatgc tatgttgtga
     1741 ccgttctgtg cgtcctcctg tatgattgct tcgtgattga tcggcgtatc ttccggtctg
     1801 tctgtcttgc ggttgtccta cacgtcttcc tacgtgtctg actatatttt ttccagtccg
     1861 gtaatccacc agatacggat ttcttcgcac ctgtatcatt tccagatccg gttgtctgct
     1921 cgaacgattc tcctgtgtca tattgctgcc ccccagagtt aattgctttc tttgagtccc
     1981 agtttaatcc ggagttgtca catttttcgg cacatattcc ttaagatttt ataaatttat
     2041 tgtttaaaaa gttccagcaa atacttttct atcacccgtg ctgctttgcg atgagctttc
     2101 actccgggat gcaggcggct gcctacggtg tccggtgtgg tgttgggaag ttgtaaaaag
     2161 tggattctgg tatcaccagt ctgttccgcg taggtttcca ccgcttcacg gatgacgggc
     2221 aggaaattgt ctcccagcat accatagccc cagatgatct gtgcctcagg attgcagtca
     2281 cgcacagctt tcaggaaatc tcggattgct gtagtcagga tctttttgac ttcttttatg
     2341 tcacgggttc ctgcccattc tcttaccagt tccgctgcgg actggattgc tgtggcatcg
     2401 ttggttccca gattaatgat cacggcatcc ggctgccatg ccttgaaatc atagtcttcc
     2461 agtgctccca gagaagcatt tcgctccccg gtaagcaatc cgcagacctg tttataatag
     2521 ggaggaatct tattctctac aatgccatcc cagccggtga cgattcccca gccactctgg
     2581 gacaccacac gatattccgc agaaagcgca tccgatacca tacggggata cgtattttcc
     2641 gcagagaaga acgcactgat ccagtcttcc tcacctaccg gccccatagt tccctctcca
     2701 ctggtaatac tgtctcctac aaattccagg cgatattcag gatccggcag aaccaaaaat
     2761 tccccatctc catattgcaa tcccaaaatc tgtatcatgt gcagcgggtc ctggtgcata
     2821 gcctgtactt ccttcagaat ccgtacatgc ttcggctttc ctactgtcat tcctcgaaac
     2881 aggcatactt catttttccc tttattaacc ggaaaacggc tgatctgggc tccattcagt
     2941 tctaccgcca gccatggctc catcgtgtcg taatcagcat ttacttctag ccacaattca
     3001 ctgccagtat atagaaattc tataccgctg ccggtccaga ataaattgat cacatctttt
     3061 cccgtggtcc ggcccaatac ccgcacttgc ggcaaatccg ctatacttat aatctttttc
     3121 tgctgttcca ttttcccata ccttctactc tgttttctgt tgcttcgaca ttttctgtta
     3181 tgatctttcg attgcctgct ttactctctc ctgccgtact cttctgcgat cacattctcc
     3241 gcaggacgtc cgcataccgc ataagggctc aatccttcca aatttcccgg ccaatcccag
     3301 atgccgaagc ctttcaccca gtcacgtttt ctgcaggctg taaacattgc ccggtaccag
     3361 tccgcctgtt ccgcatcatc ctcttcaccc tgcagttccc agttattcgg aacctgcgca
     3421 gaaccatgaa tattcatgca acccgcttcc gaaaacaaaa agggcttctg aaattttttc
     3481 acaacctttt cgatccggtc taactggttg tcccagtcgt ctatgggata atatccgctg
     3541 gacgcaatca catccacggc atcccaccat gtaatatgat cttctccata tttgtcgcaa
     3601 ttataaccga caggtccgtc atataccgtc cgtacttccg cgataagttt tctccactct
     3661 gtctcccggt gctccgtcat ggtcatttcg caccccgcca agaacaattc acagcctgcc
     3721 cgctgcgcaa tctgtgcata atgtttctga aaggcaatgt gactctcgaa ccagttgccc
     3781 catttgggtt cgcaaggtac atcatggtca aaaaagctga ttcgtgcccg ccagacacca
     3841 ttgtcacaat tcaccgtcgg tttcaaagca actttcagtc ccaggctctg tgcatatcga
     3901 atcatatcat acagttcctc atccgtacag cttttctccc catcataacg gatttcctca
     3961 ctgtaaggtg tttcctgcat ccccgatggt gtgaatatca cccaattcgt atggatcctt
     4021 cgtaccattt cctccatact ttttctggat tctcccgaac ccaatactcc tctgtgcgga
     4081 aaaaccgcaa aattcacagc gcgaaatgcc tccatacaat accttccctt ccaagtatcc
     4141 catttgtgtt tcatttcctg tattgtatca tttttaattt tatttagcta gtaaaattag
     4201 cttttatttg cctaaattta ttttcctgca tctcattgtg ttcattcaca cgatatgcta
     4261 ccatgaaact atatacagtg ctttcagaaa ggagctatct gccatgcatt ttgtacaggc
     4321 aaaggggatt ctgtctcaga ataacggaat gaatatttac cgcggatgct ctcacggctg
     4381 catctattgc gacagtcgca gtaagtgcta cggctttaca catgcttttg aagatatcga
     4441 agtgaaagga aatgcaccgg agctgttgga acaagcgctg cgctccaagc gtcacaggtg
     4501 tatgattggt accggtgcta tgtgcgatcc ttatcttccg gctgaaaaag agctgcgatt
     4561 gaccagaaaa tgtctggagc tgatcgatca atatgaatat ggtgtgaccg tgctcacaaa
     4621 agccagcctg gtgcttcggg atctggatct gctgcaatct attaatcaga aagccaaggc
     4681 agtggttcag atgacgttta ctacttatga tgaggatttg tgccggaaaa tagagcctaa
     4741 tgtctctacc acgagagaac gtttcgaggt gctgatgaaa tgtaaggaat tgggaattcc
     4801 cacccttgtg tggatgactc ccattcttcc ttttattaat gatacccgag aaaatatcga
     4861 ggggcttctt gactattgcc tgcaggcgga tgtgaaggga atcctgctgt tcgatgtagg
     4921 agtcacgttg cgggaaggag atcgggaatt tttctatcag gctctggatc gtgattttcc
     4981 cggcattcgg caaaaatatg tccatactta cggaaatgcc tatgatattc ccagccctca
     5041 cgccaaagaa ctcatggcct taattcgcaa tacctgctca gccaatagta ttctatgctc
     5101 cccgaaggaa tgcttcgctt acctgaatga attccctgag agatatgtgc agcagacatt
     5161 gttttaggtt ccaatggcga taatttctac aaaaaagttt atatagacaa cacagtaaaa
     5221 tcgggagctt ttgctcccga catcttgata acgcaaaaaa cgaatattta cattcagaat
     5281 agaactacac tcagaaaatt ggtagctata gcttcagcgc ccagaacaaa aacggcattg
     5341 tgatcacgga aagcaatgtg gtcagagcca cgcccctgga tgtcaaccga taatcgccat
     5401 tgtactgctg tgccaacatt actgtcatgc tgcctaccgg tgttgccatc attaccagac
     5461 aaacgcctcg gaatatcgga tccttgataa aaaagcacag tccccacaga agcaatgccg
     5521 gcaatagtac cagttttact acagagaaaa cgagtaggcg cacatctgta aacaaatctc
     5581 ttaaccgggt ctgcgccaaa gagtcaccaa tgaccaacat gcttaacgga cctgtgatgg
     5641 caccagtgaa agttgctgta tcttcaagga tcaatgggac cggaagattg gtgatataca
     5701 gaattatcgc aataatgcaa gagcctacgc ctatattcac gagttgtttc caatttatat
     5761 ttttctgaca accaccaatc tgcattttgt ggatgccata agtatagatc aagacattat
     5821 ataacaagtt gaagattaca gcgtgaatta ttgcattgct gccatacatg acatccagca
     5881 agggataacc cataaatcca atattggaaa aaagcgtcat gacctgataa gcaccgtagt
     5941 cctctctgtc tgcatgaagc aatctgggca atagttccgc gacagcaagc ataattgcaa
     6001 aatagatcag agcaacccct agagtaaaca acaattttcc tttattctcc agcctctcag
     6061 gattcatacc agaggcaatg atcagcgcag gatttccaat attgattact atacttgaaa
     6121 ttgttttgcc tgtagtatct ttaaacacac cg
//
LOCUS       DAWEJW010000125         6050 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3978_length_6050_cov_71.351293, whole genome shotgun sequence.
ACCESSION   DAWEJW010000125 DAWEJW010000000
VERSION     DAWEJW010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3978_length_6050_cov_71.351293"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1336 a         1747 c         1367 g         1600 t
ORIGIN      
        1 aaactatgag cgtaaacgga gttacatcaa cccaggccgc tgcagcctac agctataaca
       61 gcacaagtgc agcaaaagag aaaacttccg cagaggaagc aaaaactaca actaccgcgg
      121 aagataccgg tgtcatctat gagcacagca ccgctgccaa gacttcttcc acgaagaaaa
      181 cctatacacc ggataccaat ctggtcaaca aattaaaggc agatgcggaa aaccgtgcca
      241 gtcagcttcg ttctctggta gaacagatga tgggcaagca ggctaacacc tacggcaatg
      301 ccaatgacat ctggtccttt ttacgttccg gtaatttcac cgtagatcct gctaccaagg
      361 cacaggcgca ggcagatatc gccgaggacg gttactgggg tgtgaaccag acttccgacc
      421 gtatcattca gtttgccaat gctctgaccg gcggtgaccc ggacaagatc gagtccatgc
      481 gcgaggcatt caagaagggc tacgcacagg ctgagaagac ctggggcggt tctcttcccg
      541 agatctccca gaagacttat gatgctgtta tggagaaatt cgacaagctg gcagcagatg
      601 ccggactgac cacagaagaa taatgtcgta ttctcgacag actttcctat agaaaaacca
      661 tgttgtaccg atgccacaga acgcatccat gtacaacatg gtttttcttt tccgaactgt
      721 atcaggccag accttttacc agcagcacct tctgccccgg gcgtaattca tcgctttcca
      781 gcgcgtttaa ttctcttaca gtcttcaccg gcatatgata tttcctgccg atgctccaca
      841 ggtcatcccc cggtgccgca atatagatca ccgcactggg cagtccggcc agcacattac
      901 tgtcgatttc ccgctcttcc accccttcga tggcttccaa cggaatattc ttcatcacgg
      961 tcgtggaaaa ggaaagaaca gcctttacat ccagttcctc tccctccagc agattcacct
     1021 gcagctgctc cagttcactg tgtacctctg cctgatccgt tcccgtcact ccctgcacat
     1081 tcagggtata ttcatacgga atctgctctc tggtactcac ataaggagtc tcatcatttt
     1141 ccgtaatgca caacactgta agatcaatgc tgccctgcag gcggattcct tcctctgtgg
     1201 tctcctgatg atcgatcgcc acgattccct cactgtgcaa cacctgcagt accggtgagc
     1261 cgatatcggt ctttctgtga tccgtcactt ttgtcttgcc tgtgattctg gccagcaact
     1321 gacgcaggct ggcatctctg gtgtccggga tcacttcttt tgtcacgccg taaatatctc
     1381 ttaaaatatc gatctgctct tcctcataga gacggatatt cagttccaga gcacattcca
     1441 gccccaggat tctctcttcc ccgtccagat ccggctggat cgtcagctct ggctgtccgt
     1501 gttcctgtct taccagtgaa taccggatgt ccggcagcag ttcctcccga catccctgac
     1561 attccagagt gccattcagc ggaattgtcg tctcatagga acgaatagac cgctcctccc
     1621 cctctgcctc atagagaacg aagacctgaa tatctccctg cactgccagt ttctcttcct
     1681 gtggcttaaa ttccacatcc cgcagtgaca cattactcca gaggatctgg taaatattcg
     1741 gatagctgga gggtagcgga atctcctctt ttttgcgaaa aatatcgttc ttgcagatgg
     1801 aaagctccgt cacctccatg ggcgagatcc ggtattccac accttctccg gcgctgcctc
     1861 cctggattcc cacgggtaat tcttcatcgt aaatctcttc gatccgggcc gtgagaagca
     1921 gcaatgcctg cagattcagc ttccgggaat tgatcatatt taccgtgaaa tcttctacgg
     1981 ttccattcac cgctacagta tctgacggca acgttccctc catatgaatt ttttcttcaa
     2041 agggcagtgc accgcttaaa agcaccagat tgctccctgt ctcctgcgta tgatacagaa
     2101 tggcataacc cagttttccc cgtaccgtca cctcatccgt accgggccgg atctcctcga
     2161 tgacaacttc tcccttttcc aggttcaggg tactgatgtc cggtttatcc tctggaagat
     2221 tcagatcctc ctccatggta aactgtatga cagtggatcg ttttatccgg tccatatgta
     2281 tatttctctt gatcagctcc acaataaaag acctccatat atacagatat atctatatat
     2341 atgaaggtct ctgtgtactt ataccgtatt ctgttctctg tatcttactg cactgcaccg
     2401 atcagatcgg tcagattctc cacatggtag gtacgcatgt aatcctcgat gccctccact
     2461 actttcacgg tagtataggg atccacaaaa ttcatggctc cgacgctgac agcgctggca
     2521 cctgccagga taaattcgat ggcatcctcc gccgtggcaa tacctcccat accgataatg
     2581 gggatctgta tcgcctgtgc cacctgatag accatacgga cagcgatggg tttgatggca
     2641 gggccgctca tgccgccggt cttgttggca atggcgaatt ttctgcggtg aatgtcgatc
     2701 ttcataccgg tgatggtatt gatcagggac agggcatctg cgcctgctgc ctccgctgcc
     2761 tttgccatct ccgtaatgtc cgttacgtta gggctcagct tcatgataat gggctgtttt
     2821 gcatgatttt tcagttcgga agtaatattg tacagagcat ctgctttctg cccgaacgca
     2881 atggctcctt ccttgacatt gggacaggat acattgatct ccagcatgct gactgccggt
     2941 tcgtctccca gtcgctctac cacatccacg taatcctcta cggtcttacc gcagacgttc
     3001 acaatgatct tcgtatcata ttttttcagg aaaggaatat ctctctccat gaatacgtca
     3061 atgccggggt tctgcaaacc gatggcattg agcataccgc cgtatacttc tgccacacga
     3121 ggggtcgggt tgccgggcca gggcacattg gctacaccct tggtcaccac cgcacccagg
     3181 cggttcaaat ctacgaattc ttcatattcc atgccggagc caaaggttcc ggatgctgtc
     3241 attacggggt ttttgaaagt tactcctgca atcgttgtct gtgtattcat cagatctcta
     3301 cctcctcagc atcaaatacg gggccatcgg tgcagattct cgcattatgc acatgagaat
     3361 gatgatccac ttctttggtc tttaccacgc agcccaggca ggcacccacg ccacaggcca
     3421 tatgctcttc cagagagata taggccttga tttctttttc ttccgcatat tttttgatcg
     3481 cacggagcat gggcatcggt ccgcacgcga agatcacatc tgcctgcaat gcattttctt
     3541 cgatggcatt catgacattg cccttcgtgc cgacactgcc atcctcggtg gcaatatata
     3601 cggttccgtt ctcttccaga tcctctttta agaacagctg gttatcacgg tagcccacga
     3661 tgatctgctt ttctgcggaa gccggaagct ctcttgccag ctccagcatg ggagggatac
     3721 cgatacctcc gcccataagg aatacttttt tgccttccgc tgcctctgtg gggaagccgt
     3781 tgcctaaggt tcccagaatc tccacactgt ctccttctgt ataagaagaa aattccgcgg
     3841 tgccctgtcc ggcaatccgg tacacaatgc gcagggcatc ctttttgtcg cttacctgac
     3901 agatgctgat aggacggggc agcaaggtgc tccggtcctt ggggtatacg ccgatgaact
     3961 gaccggcttt tgcctgttca gccagatctg tagcgatcca catgtcaaaa attctgggtg
     4021 cgatctcctt ctgggaaagc acagttgctg tgattttctt tttcatgtta ttgtcctttt
     4081 ctcttctatt tataacgcta tagcgctctt ataagtgctc tctaccatgg gctctacgct
     4141 tcgtatacga ttctgcctcc gcagatggtc ttcctcactt cacccttcag attccatccg
     4201 gtaaacggtg tattcgtggc cttggacgca tatttctccg gagtgaactc cgctgcggta
     4261 tcgatcagaa tcacatccgc cgggccgtcc tcggccagat atccggcatc cagatggtac
     4321 atagccgccg gattggtact catcagacgc aacagctgct tcatggtcag atatcctctg
     4381 tccaccagtt ccgtaatgcc cagcgccaga gacgtctcca acccggtaat gccgctgggt
     4441 gcctctgtga tcggctttgc tttttcctcc gcagtatggg gtgcatgatc cgtagcgatc
     4501 atatcgatgg tgccgtccac cagtccccgg atcaccgcct gacggtccgc ttcctcccgc
     4561 aggggcggat tcatcttcgc cagtgtaccg tacttgatca cggcatcctc agtcagggta
     4621 aaatgatgag gagtggcttc cgcgtggata ttggagcagc gctttttggc ctgacgcacc
     4681 agctccaccg cttccttgct gctgatatgc tgaatgtcga tgcaggctcc ggtctcttct
     4741 gccagcttca ggtcccgctc caccagcgcg atctccgcct ctctggcaga accgccgata
     4801 ccgtaatact cgctggcctt tccccggttc acaccgttgt tctcgatata tttcggatct
     4861 tcttcatgga agctgatggg catgtccagc tctgcggaga tctccatggc acggcgcacc
     4921 agttccgcat ccagcagggg cactccgtca tccgtgaatc ccacggcacc tgccgttgcc
     4981 agcttcttca tatccgtcag ctgttttccc tgcatcccca tggtcacatt ggcgcaggtg
     5041 gtcacatgaa tgcctgtctt cgctccctta tccaggatat aatgcagcgt ctcctcactg
     5101 tccaccgtag gcttcgtgtt cgccatcagt accactgtgg tcacgccgcc ttttgctgct
     5161 gccgcagcac cggtgttgat atcttccttt gccgtaaagc cgggatcccg gaaatgcaca
     5221 tgcacatcca ccagcccggg agccaccaat aacccttccg cattgatccg ggtcactttg
     5281 cccctcggtt ctatcttttc cgcaatcttt acaatcttgt caccgtcgat caggatatca
     5341 taattgccct ctctgcccga cttgggatcg atcatgtagc catcatgtat cagtatcata
     5401 ttgtctccat tcccgggctt ttaccacccg ccttcgtgac ctgtttctcg gaaaagttta
     5461 tcatgttttc atagaaaaga aaagagtgtt ataatagaaa aaaggaggtc tgacgacatg
     5521 aaaacaagag aggaagcgct gaactacgga ttgtcttttc caaatactta tatggaaatg
     5581 cccttccacg atcccaactg gcagttggta cgtgtgaagg gcagtaaaaa agcttttcta
     5641 tggacttatg aaaaggatgg cttcctgcat ctgaatgtga aaacagatcc tgcctggcgg
     5701 gatttctggc gaagcacctt tccctccgtc atcccgggct atcatctgaa caaagagcac
     5761 tggaacacca ttattttaga tggttcgatt cccgatgagg acatccggcg catgatcgca
     5821 gaaagctatg atctcgtcac cgacagtcct acgaagcgga tctatgaagc cgtcaaaaag
     5881 attccgaaag gctgcgtcgc cacctacggt caggttgccg ccatggccgg agaaccgaag
     5941 atggcccggg ccgtgggcaa tgccctgcac aagaacccgg atcccgacca tatcccctgc
     6001 taccgcgtgg tcaattccaa aggggaactc tccggtgcct ttgccttcgg
//
LOCUS       DAWEJW010000126         6036 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_3984_length_6036_cov_56.980605, whole genome shotgun sequence.
ACCESSION   DAWEJW010000126 DAWEJW010000000
VERSION     DAWEJW010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3984_length_6036_cov_56.980605"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1557 a         1505 c         1379 g         1595 t
ORIGIN      
        1 ttcctcctat ataacaaagg cgttctcctg cccgtttatt gtaacaaaag aacgccgttt
       61 tgtgtatgat cttttacaaa gttacaccct gtttgaaaat agccatatca tggaagcctg
      121 ctgcttcgct gcatacctgt ttgccggaag cagtctccac taccagatca aacaaagcct
      181 tggtctcctc ttccatggtc ttatcctcta ccagtacgcc ggcattgaaa tcgatccaat
      241 tggacttttt gcctgccaga cgggagttgg tagcgatctt catggtagga accggagatg
      301 cgaaaggagt tccacgtcct gtggtaaaca gtacgatatg tgctcctgcg gctgccagtg
      361 cggtagctgc taccagatcg ttgcccggtg cggacaaaag gttcagtccc ggagtcttca
      421 ccgtatcacc atactgcaat acacccctga ccagggcact tccggacttc tgggtacatc
      481 ccagagactt gtcctccaga gtggagatac cacccttttt gttgcccgga gaaggattct
      541 cgtaaatagt ctgattgtga ctcttgaaat aattcttgaa gtcattgatc agatctacgg
      601 tctggtggaa cagttcctcg ttggcacaac ggttcatcag aatggtctct gcaccgaaca
      661 tctcgggaac ctcggtcagg atggtggtac cgcccttaga gatcagcagg tcggagaaac
      721 gtcctaccag agggttggcg gtaataccgg acagtccgtc actgccgccg cacttcatgc
      781 cgatgaccag cttgctgacg ctgatgggct ctctctcgaa tttggatgcg taatcgatca
      841 gacccttgat gatctctacg gagtctgcga tctcatcctc gtgctcctgg cataccagga
      901 attttacacg gttctcatca taatcaccga tataaggctt caacacatca atgttgctgt
      961 tctcacagcc cagtcccagt accagaacac cgcctgcatt gggatgattg atcaggtctg
     1021 ccagaatctt acgggtatgc tcctgatcgt cgcccatttg ggaacatcca taaggatgag
     1081 gaaatgcaat aacctcttcc acactgcctt taacgaatgc attggcctgc tttgcaatgg
     1141 cggttgccac gttgttgaca cagccaacgg tggggatgac ccagatctcg ttacgaacac
     1201 ctactttgcc gtccggacgg ttgaagccca taaaagtcac atcttccgtc ttcttctcct
     1261 ctaccggagt ggggttgtag gtgtactcca gcagatcgcc cagagcggtc tttacattat
     1321 gagtatggat ccagctgccc tctgtgatgt tctccttggc attaccgata cggtaaccgt
     1381 atttgatgac ctcgccgcct tcgggaatat caaaaatagc catcttatgt cctgctggga
     1441 tctcctctct ggcggtaacg gtcttctgac tgtcacctac agagactgtg atcttctcac
     1501 cggcaggaat cgtatttaat gctacgacta cattatcatt gtcattaatt tttaagaaat
     1561 cttgcataat gctgccctca tgccatttgc cttaatatca tccaggtatg ctacaactgc
     1621 atcggtcagt ccgggaacct tgttcagatc ctggccgaag aagtcttcat taccaaggaa
     1681 ggaagttacg aaagtcttgg tgtccttctc acaatttgct gcgaagaact ccagaactgc
     1741 tttgtcatcc atgatgttgt attcctgacc gtctctatga ccgatcagag ccttatcgcg
     1801 gatctcggtg ccatggtaga atgccatcag tgctgcgatg gagaaggtca gatgagcggg
     1861 aagcttaccg gtcttctcaa catagcctaa cagactgggc atacatcttg ctctccactt
     1921 ggatacggaa tttaaggaga tagcaagcag tgcatgatct acataaggat tgtcgaaacg
     1981 gtttacaacc tcacctgcaa actgcttcag ttcctcctca ggcagagaca gggtagggat
     2041 tacttcgtca taaatggtct tcagcatgaa gttacggata tcgtcatcat gcatggactg
     2101 tcttacgatg tcgttgccgc acaggtaaga agccagtacg aaagaggtat gcgcaccgtt
     2161 caggatacga acctttctct gcttataagg cttctgattg tcagtgaaga taacaggaag
     2221 tcctgcatcg ggaagaggga attccttgct gatgtcctta gcggattcga taacccacag
     2281 agcaaacggc tcaccggtta ccatgatgtg gtcttcgtaa cccagcttct cccattcttc
     2341 cttctcggta gcacggggat aaccggtaat gatacggtct accagagtag aggtgaatac
     2401 acatgcctca tctacccact tcttgaactc atcgcccagt ccccacagct cgatcagctg
     2461 cattacacac tttttcagca taataccgtt gtcatcgatc agctctacgg gaagcattac
     2521 cagacccttg ctcatatcac ccttgaaggt ctcgaatctg tgatataaga acttggtcag
     2581 cttgccgggg aaagtcttgg gaggctctaa ttcaaactta tcggtatcat catatacgat
     2641 acctgcctcg gtggtgttgg atactacgaa acgtaaggta tcgatctcgg caagtcccat
     2701 gtacttgtca tattcattat aagtgtctac agcatctgca acactggtta cgattctatt
     2761 aataatcttg gcttcaccgt tcacattacc gcgaagggat acagtatact ggcagtcctg
     2821 cttgtggaac atatccaggt taccgaactc gatgggtttg atcagaacga tgtctccatc
     2881 gaacttgccc tgctcgttag cgatgtcgat catgtaatct acgaatccac gtaagaaatt
     2941 accctcgccg aactgtacca ccttgatagg tctttccttt ttaccggtct ttgccttgtt
     3001 taagatatcc attttgtcct ccattctacg cttagcgtat cctatctgca ccgtcttacg
     3061 gcactctatt acctgcactt actttccccg gcacgcctta cgaacgaatc tgttacaccg
     3121 cccggggcat tgtctttggt ctgaattgta ggaagtttat gtaagtactt tcatgattca
     3181 ttttacttca aaaaaatggt ttcgtcaatc accaaaaaaa gaaagtttta ttttcgtgaa
     3241 actaccgatt ttatcagcaa tatttcgctt tgataaattt ttggcatatg agaagttgaa
     3301 aaagtatctt gaaaaaagag aagtctttcg ttataattca gatatagctt ttttatgtaa
     3361 ctacttacat ttttcttaca tatcttttta cacactgtta catagaagca gagaggaagc
     3421 cacagatgac aatttacgat atttctgaaa aggccggtgt ctctattgct actgtttccc
     3481 gcgtactcaa cggaagcaac aatgtaagcg agaaaaccaa gaaaaaagtg ctggatgtga
     3541 tcaaccagta cgagtacact cccaacgcat tcgccagggg attaggtctc aacaccatga
     3601 agaccatcgg tatcatgtgt gccgacagtt cggatccgta ccttgccaaa gcgatctact
     3661 atattgagca gaagcttcgg gccaacggtt acgacagcat cctgtgctgt accggttatg
     3721 atctggatac caaagcaagt tccatgaacc ttctgatcac gaaaaaagtg gacggcatta
     3781 tattagtagg ttccaacttt atctacgaga aggaagatga caacaaatac atcctcgacg
     3841 ctgccggaca ggtgcctgtg atgctgttga atgcagccat ggacgctccc aatgtttaca
     3901 gcatcgtatc ggatgatttt acttccatgt atgatgccac catggaaatg atccgttccg
     3961 gagtggaaga catcctgtac ttctacaact ccacttctta cagtggtaag aaaaagctgg
     4021 ccggttaccg tgccgccatg gaggaaaaag gacttctgac caacggaagc ttcctgcagc
     4081 tctatcaggg atctcatgaa gatattcccg ctatggcaga ccagctgctg aagctgcatg
     4141 caaagggaat caccttccac ggtgtcattg ctgctgacga ttctctggca ctgggtgctc
     4201 tgaaatacgc ccgtgaaatg aagctgcgga ttccggagga tctggctatc atcggttata
     4261 ataattccat gctggtgaac tgctgcgatc cggagctgac cagtatcgac aataagctgg
     4321 aaacactctg ccagcatctg atcacaacgc tgatgggcgt tctgggcgga agcgagatgc
     4381 cgaagaagac tgtattctcc ggtgaactga taaaacgcgg taccacaaag tagcttgtgg
     4441 gcacatatag gcatttgttt atgacagcac gggggagctg caaatcaccc cttttctata
     4501 tttatcacta tagtcgccgg atccctaccg gatctgactc atatgaacat ggaggatttt
     4561 taaaatgaaa gcatttatgg acaaggactt tctgttagag accgagaccg ccaaaaagct
     4621 gttccacgac tacgcagaaa aaacaccgat tctggactac cattgccata tcaatcccaa
     4681 ggagattgct gaggatcgtc agttcgacaa catcactcag gtatggctgg gcggcgatca
     4741 ctacaagtgg cgtttcatgc gttcttgcgg tgtggacgaa aagtacatca ccggtgacgc
     4801 ttccgattac gagaagttct gcaaatgggc tgagtgcctg ggcaaggcta tcggcaaccc
     4861 cctgttccac tggagccatc tggaactaca gagatacttc ggttacaatg gcgttctgaa
     4921 caaaaacacc gctgacgaag tatggaacct gtgcaacgaa aaactgcagc agccttccat
     4981 gagcgttcgt aatctgatca agcagagcaa tgttaccctg atctgcacca ccgatgatcc
     5041 tattgattct ctggagtggc acaagaagct ggctgcagat gatactttcg atgtaaaggt
     5101 tctgcccgca tggagacctg acaaagctat gaacatcgag aagcccgatt atctggatta
     5161 tctggagaag ctggctgctg ctgcaggcat gaccgagatc aactccttcg caagcttaaa
     5221 ggaagctctg aagaaccgta tggcattctt cgcatccatg ggttgtaacg tatctgacca
     5281 tgccctggag tatgtaatgt actatcccgc ttccgacgat gagctggaag agatcttctt
     5341 aaagagactg aacaagatgg ttctgaccaa ggaagaggag ctgaaattca agaccgcctt
     5401 catgctgttc gtaggcaagg aatatcacaa gcttgactgg gctatgcagc tgcattacgg
     5461 ttgtaagcgt gacaacaaca ccctgatgtt cgagaagctg ggtcccgata ccggttacga
     5521 ctgcatcaac aactacgctc cttccgcaca gatggctgac ttcctgaacg ctctgatcgt
     5581 aaccgacgag ctgcccagaa ctgtcatcta cagcctgaac cccaacgaca accaggccat
     5641 cggtaccatc ctcggatgct ttcaggattc caccgctgtt gccaagatcc agcagggttc
     5701 cgcttggtgg ttcaacgatc acaagaccgg tatgcaggat cagatgatct ccctggctaa
     5761 cctgggcaac ctgtccggat tcgttggaat gctgaccgat tccagaagct tcctgtccta
     5821 cacccgacac gagtacttcc gtcgtatcct gtgcaacctg atcggtaact gggtagagag
     5881 tggtgaattc cctgcagatt acgatactct gtccgagatc gtaaccgata tctcctacaa
     5941 caacgctaag agatatttca agttccctct ggcataaaat tataagaata gtgcaatccc
     6001 ccggctggtt atttggccgg gggtttttat gtaaat
//
LOCUS       DAWEJW010000127         5922 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4073_length_5922_cov_65.790523, whole genome shotgun sequence.
ACCESSION   DAWEJW010000127 DAWEJW010000000
VERSION     DAWEJW010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4073_length_5922_cov_65.790523"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4967..5066
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1600 a         1162 c         1672 g         1388 t
ORIGIN      
        1 ttttcataac ttgtttgtgc cttgagcgaa gcgaaaggaa tgacaaaaac tatgaaaaaa
       61 atagcaattc tggggtctac cggctccatc ggaacccaga ctctggaagt cgttcgcaat
      121 aatccggaat tgcaggtggc agcactggct gccggaaaaa gcgtagaaca gatggaacag
      181 cagatcagag aattccatcc tctgatcgca ggcatgtgga gtgaggaagc ggcagcagac
      241 ctgcggagcc gtgtggcgga tttacctgtg aaggtcgtaa gtggcatgga tggtctgttg
      301 gagatcgcaa caatgccgca gtcccaggta ttagtgacgg cgattgtggg aatgattggt
      361 atcagaccga cgatcgcggc cattgaagca gggaaagata ttgccctggc taacaaggag
      421 acgctggtaa cggcagggca catcattatg ccgttagcgg cgaaaatggg tgtgaagatc
      481 ctgccggtgg acagtgaaca tagcgcgatt ttccagtcac tgaacggaga accggccgga
      541 agaatcgaga agatcctgct tacggcatcc ggtggaccgt tccgcggcag gaccagagag
      601 cagctgcaaa atattcaggt ggaggatgct ttgaagcatc ccaactggtc catgggacgg
      661 aagatcacca ttgattcttc cacactggtg aacaaaggtc tggaagtcat ggaagtaaag
      721 tggctgttcg gtgtggatct ggatcagatc caggtcattg tgcatcccca gagcattatc
      781 cattccgcag tacagtatgt ggacggagcg gtgatcgcac agttgggaac tccggatatg
      841 aaactgccga tccagtatgc gctgttttat ccagatcggc gtccaatgcc gggaaaacgt
      901 ctggacttct atgaattggc acagatcaca tttgaaaagc cggatatgga gacattcttt
      961 ggcctgaagc tggcttatga tgcacagcgc atcggcggca gtatgcctac ggtgtataat
     1021 gcagccaacg agaaagcagt aggactgttc ttagaccgta agatcgctta tcttcagatc
     1081 ccggagctga ttcgtgaggc catggagcag cataaggtga tcgagaatcc gaatgtggag
     1141 gagatcctgg agacagaggc atctgtctat gattttattg aaaaagtgta ttctgagagg
     1201 caataaatgg ttactttgat cttgtttgta ttgatctttg gggtggtggt aatctcacat
     1261 gaattcggac attttctgct ggcgaaggcc aatggaattc atgtgataga attttccgtg
     1321 ggtatgggcc cgaatctgtt ttctttccag aaaggagaca cgaaatattc cctgaagctt
     1381 cttcccatcg gcggagcctg tatgtttgag ggggaagacg gtctgaatga gaaggaggac
     1441 ggtgaagatc acagcggatc tttcctgaac gccaatgtgt gggcgaggat ttccaccgtg
     1501 ttggcaggac cgatatttaa ctttattctg ggctttatca ttgcctttat tatggtgaat
     1561 ctgattgtga tccgtgatcc ggtagccacg gagatcgtgg atggcggcgc cgcacaggaa
     1621 gcgggactgg aggccggaga caggatactt tccttaaatg gcagtaaaat acacctgtat
     1681 gaagaaatac agttatttac tctgacttac cgtggcggag aagtagcggt ggaatatgaa
     1741 agggatggtg taaaaggaac tacaacgctg acaccgaagt acgatgagag cgaaggccgc
     1801 tatctgatcg gcatcagtaa tgcggatttc gtagaactgt ccggattgga cagcttccgg
     1861 tattcctggt atgagatgcg ctactgcgtg aacatgacct ggaagagtct ggcgatgctg
     1921 gtgcaggggc aggtctccag gcaggatgtg gcaggccctg taggcatcgc ggtaaatgtt
     1981 gttggcaaga cctacgaaag taccaaggat tacggctggc agaatgtact ggtgaatatg
     2041 ttgaacatca ccctgatgct tactgttaac ctgggaattc tgaatcttct gccgatcccc
     2101 gcgctggacg gcggccgact ggtattcctg atctgggaag ccatcacaca caagccggtg
     2161 cctcccgaaa aagaaggcat ggtacacttt attggactga tgttcttcat ggtactgatg
     2221 gtgttcctgt tatttaatga tctggttaat atctttggat aagccggata attcaagacg
     2281 ttcaataagt aagatccgtt gacaaagggc ataccgcatg gtatgctctt tttatatttg
     2341 tgcaaggcac atttctttga aaaatttcat gccgatcggc aaaaaattcc tgatgtagca
     2401 atgtaacact gaagtaagaa acagtacgga caaaacagaa taaaaaagga ggaatgcgta
     2461 tggagcatgt gacagcctgt tattggcaaa aggaaacacc tgcagagctg tttttatctt
     2521 tgcagcaacg aaaataccgt aggctgggga tttccgtggt atgcgcctgt atctcggaca
     2581 acggggaact ggtaagaagt ctgcaaaaca aaatggagga ggaactggaa gaagagaatg
     2641 tctggaggcc gtttcgggaa gaaatattgc aggagaaatg gatggacatt ataaggcagc
     2701 aaaagcagga tgacagttat gcgggaatct tatgcgtggg gagcagagta ttgtttttca
     2761 gtcacgggag aatgcggatc tgtggatgtt ttcagagatt cggcaggaca caatggagga
     2821 cgatgcagga atcctgcatg gcgggagaag tggaaccggg gacggccatg ttgatggcgg
     2881 acaatgggtt cctgaatttc tgtgaagagg aactgacagc atgtctgcaa ccgcagattg
     2941 caggaagacc ggatgcacag gaacgcgtgg aaggcgctgc aagacggctg aaggaactgg
     3001 gagaaaaggc ggagagacac ggaggaacac acatgggagc ggtgtggatc ctgccggtta
     3061 tgggaggaaa aggatggagc agagaataac agaaaatatg ggcagatcat taaaggaaca
     3121 tggctatcgg gatccggtat ttgtgggcaa ggggagtttc gcgaaggttt accgcgtccg
     3181 ggacgaaaaa agggactttc aggcatgtaa gatatcgaaa gttacagaac agtgggaaca
     3241 ggagtgtcgt aacagcagag agatctgtca tcctctgttt ccggcctaca gagaacactg
     3301 gacagacggg gaatgggggt atctggtcat ggaattctgg gatggctgtg acttgcggaa
     3361 aatgttggac aggaggggga gactgtcacc gggacaggcg gcccggatag ctttgcagat
     3421 tacagaggga ttgcaatatc tgcatgaacg gccgcatccg tttctctacc gggatctgaa
     3481 gccggaaaat atccgcatcc gcgtggatgg cagagcgggg attctggatc tgggatgtct
     3541 gtggaaccgg gagcaggact ggtcgggagc gggaaaccga agcttcagcg ctccggagca
     3601 gttcgtgccg ggggagattc ccggggaaga gagtgatgtc tatgcagtgg gcagactgct
     3661 ggcagttatg ttgggagatg acacagacgg cacagtcaga caatctgggg gaataaggag
     3721 gcgtaatgcg gaaaaacgtt gcggacgaaa agaccaaaga caggaaaaat ggcttcgcaa
     3781 agtgatcgcc gtggcaacct gtgaagagcg taaggatcgt atcccggaga tcaaaatgct
     3841 gcgtactctt ttgatggtga cggatgattc cgggagggag aggaaacgtg catgtagggc
     3901 agctgatttt tactatgtcc ggaagttgta ctgcccatag ttgtacgttc cgtgctcgaa
     3961 cacaatcaag aaattttaca aaaaagtgct tgctgttttg gagagaaatg agtatactgt
     4021 aactaaaggt tcaaagaagc gtatgaggac cggaagcaat taaatttaca tagcaaaaag
     4081 gagaagagtt atgcgttttt ttattgacac tgcaaaagta gaagacatta agaaagcaaa
     4141 tgacatgggg gttatttgcg gagtaactac caatccctcc ctgatcgcaa aagagggcag
     4201 agatttcgta gaagtgatca aagagattgc atccatcgtg gacggaccta tcagcggtga
     4261 ggtaaaggct accaccgtag atgcagaggg tatgatcaaa gaaggaagag agattgcagc
     4321 gatccatccg aatatggtcg ttaagatccc tatgactgta gaaggtttaa aagcatgtaa
     4381 ggtattaagc tctgagggaa tcaagaccaa tgtgaccctg atcttctctg cgaaccaggc
     4441 tctgttggct gcccgcgcag gtgctaccta tgtatctcct ttcctgggca gactggatga
     4501 tatcagcacc agaggtgtag atctgatccg tgatatcgct gatattttcg cagtgaccga
     4561 tctggatacc cagatcatcg cagcaagcgt gcgtaatccc atccatgtaa ccgattgtgc
     4621 actggcaggt gcggatattg ctaccgttcc ttactccgtt atcgagcaga tggtaaagca
     4681 tcctctgact gatgccggta tcgccaagtt ccaggcagat tataaggcag tctttggtga
     4741 atagtctgtc aatgcatagg cgggacgact gcttgcagtc aggaacgcct atgcattaga
     4801 cggacaaaca gacatgagca agtagggcga cagcccggaa tgcgaatgtc tgtagcggtt
     4861 cgagagtgcg aagcacggag aagcgcgtga ttcaccaaag aagacgcact gtgagcaagt
     4921 agggcgacag cccggaatgc gaatgtctgt agcggttcga gagtgcnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnngaag cacggagaag cgcgtgattc accaaagata
     5101 ctgacacagt aagaagctgt acagagaata tctctgtaca gcttcttttg ggttctgtcg
     5161 attccggcag agcttatttc atctgctctt cctgcttctt gatcatacga cggaccatct
     5221 caccgccgat gcttcctgct tccttagaag taaggtcgcc gttgtaaccc tctttcaggt
     5281 taacacccag ctcagatgct acctcagtct tgaaacggtc cattgcagcc tttgcttcgg
     5341 gaacttctac tctattggtt ttacttgcca tttcgttcac ctccgtttgg atttgggttt
     5401 tcttgtttca gataagcgct ttttgtgatc acttatcttg gtattagtat gagcatcggc
     5461 ttttttttca tgctggtaag tttttcattt ttatgttaca ctcaaacagc aacgaaggat
     5521 acggagaccg gaaagcgtga ccaacgatcc actgaccgga atcccacaga agtgactgac
     5581 ctttaaagct acatcgtaac tgcggaagct gagaagagaa ggtgtttttt tgttgtcaaa
     5641 atgtaactat tcagaacccc ttcgaataag aaggctgata cttcatgctt gcatgcaagt
     5701 agcagttttc ttattcgcaa ggtggcttga atagagtcac tgccccgtac atgagcaaaa
     5761 ttagctgcct tgcagcgaga aagcacgatt tatcgtgcgg ttttgcgaat gctggggtgg
     5821 gctgaatagt tacattgtaa ctattttatg acagaaaggc gaaaaagcaa tgaaaaagaa
     5881 aacaaaaatc acaaaaactc tggcacttgc actgactctt gg
//
LOCUS       DAWEJW010000128         5894 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4098_length_5894_cov_67.617058, whole genome shotgun sequence.
ACCESSION   DAWEJW010000128 DAWEJW010000000
VERSION     DAWEJW010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4098_length_5894_cov_67.617058"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1415 a         1522 c         1367 g         1590 t
ORIGIN      
        1 gtaaggaatc gtccttgttc gtgccggtga tcttcgcctg ctgctcacgc tctaccttct
       61 gttctatctt gatatggaac agaagtctca cagtctcttc ccggatgttc tgagtcatct
      121 catcgaacat ctcgtaaccg ctcatcttgt actctaccag aggatctctc tgaccatatg
      181 cctgtaagcc cacgccctga cgcagctgct ccatgtcgtc gatgtgatcc atccacttgc
      241 ggtcaatgac cttcagcaga accacacgct ccagctcacg gatggcttcc ggttcgggga
      301 actcggcttc ttttgcttcg tacagcttca cagcttcttc ctttaactgc tgctttaagc
      361 tgttcttctt gggcttcttc acacgctccg gtgtcagagg ctgtaacggg attgtaggaa
      421 tgagcagggt atttaactca ttgaaatccc agtcggagat atctgcgtcg tcattgatac
      481 agatatctac acagttctcg gtaatatcgg tgatcatctt gtagatcaca tcacgcatgc
      541 tctcgccgtt cagtacccgc agacgctcct cgtagatgat ctctctctgt tcactcatga
      601 cacggtcata ctctaacaga ttcttacgga taccgtagtt gttgctctcg atcttcttct
      661 gtgcggattc gatggcattg gtcaatgcct tgtgctcgat ctcctgtccc tcagggatac
      721 ctaaggtatt gaacatgctg atcaggcgct cggaaccgaa cagtctcatc aaatcgtcct
      781 ccagagagat gtagaacttg gactcaccgg gatctccctg acgtccggaa cgaccacgca
      841 gctgattatc aatacgtctg gactcatgac gctcggtacc gatgatcttc agtccgccgg
      901 cggctctcgc ttcatcatcc agcttgatat ccgtaccacg gcctgccatg ttggtagcga
      961 tggttaccgc accgtgtaca ccggcatcgg ctacgatctc tgcttccagt tcatggaatt
     1021 ttgcattcaa taccttgtgc tggatgccgc acttggtcag cattctgctc agttcctcac
     1081 tggcttcgat agtgatcgta cctaccagta caggctgtcc tgtggcgtgt gcttcctcta
     1141 ccgcattcac gatagcgttc agcttctctc tcttcgtctt gtatacagca tcctgcagat
     1201 ccttacggat aacgggacgg ttcgtaggga tcacaacaac atccatgccg tagatctcac
     1261 ggaactcggt ctcctcagtg agagccgtac cggtcatacc acacttttta tcgaataagt
     1321 taaagaaatt ctggaaggta atggatgcca gcgtcttact ttctctcttc acctttacat
     1381 gctcttttgc ttcgattgcc tgatgcagac cgtcggagta acggcgtccc ggcatgatac
     1441 gtccggtaaa ttcatcgacg atcagtacct ggtcatcctt taccacataa tccttatcac
     1501 ggaacatcag attatgggca cgtaatgcca gaatgatgtt gtgctggatc tccaggttct
     1561 cgggatcagc gaagttatca atgtggaaga atctctcaac cttctccata cctgctgcgg
     1621 tcaggttaat gatcttatcc ttctcgttga ctacgaagtc tccggtctct tcctgtacca
     1681 cacccataat ggcgtccatc ttggtcagtt cctgtacatc atcaccgcgc ttcatctgac
     1741 gtgccagcag atcacacatt tcatataaag cggtagactt gccgctctgt ccggaaatga
     1801 tcagaggagt tctcgcttca tcgatcagta cagagtccac ctcatcgatg atggcataat
     1861 gcaatccacg cagaaccagc tgttccttgt aaataaccat attatcacgc agataatcaa
     1921 atcccagttc gttgttggta acataagtga tatcacactt gtaagcttcc tgtctctcct
     1981 cggaggtcat gctgttcagt accacgccga ccttcagtcc caggaactca tgtacctgac
     2041 ccatccactc cgcatcacgc ttcgccagat agtcattgac ggtaacaaca tggacaccct
     2101 tgccttccaa agcattcaga taagcgggaa gggtagacac caaagtctta ccttcaccgg
     2161 tacgcatctc tgcgatacgt ccctgatgca ggatgatacc accaatcagc tgtactctgt
     2221 agtgttccat gttcagaaca cgctttgcag cctctcttac ggttgcataa gcctccggaa
     2281 gcagatcgtc cagagtctct ccctcggtga gacgcttctt gtactcttcg gtcttccccc
     2341 gcagctcctc atcggaaagt gcctgcatcg cagggcggag tgcctcgatc ttgtccacca
     2401 gaggttcaat acgcttcagt tctctggaac tgtgcgtgcc aaatatcttc tcaataacgc
     2461 tcattttttc ctcgtttccg cacccttttg acaataaaat gtccatgggt cttcgcataa
     2521 ttcaaaggtt attgtagcag attttcctct atgtttcaac ctttcaatgc gcccgttgcc
     2581 gtgaaaagac tgtaaaaatt ttatgaacag ctctcttcat ctattctgtc ccggaaaatt
     2641 gacattccgg ggaaaaatta ttataattga aataagtagg atttacgaaa gcacctgatg
     2701 attttcaggt taccgaaagg cacaaggatt acggtctatg aagagattat ccatcacaca
     2761 attgattccc ggaatgattg ttgcagaaga tgtctacaat ctggatcatg aacttctgat
     2821 tccgaagggc acggttctca cggacaagct catcacgaag ctggatctgt acggtattct
     2881 cacaatcttc gccgaagatg tactcccggc atcggatctt tctgaaacgg aatcctccca
     2941 ctatgaacgg atcaaaagtt ctccggaatt tcatctgttt aaagaagatt ttgagaatga
     3001 agtcgaactt ttccgggaaa atatgaatct ggtcgttcag aagaatacag ccctggatgt
     3061 gaaaactctg ctgaaaaaca cactttccat tgtttcgaat cattccggca gcatcagtat
     3121 tctggatatg cttcaaaata tgcgggaata cgatgattcc acctataccc acagtctgaa
     3181 tgtggcactg atctgtaata ttctggcggg atggctgaag cttccgaaag aagacgtgga
     3241 actggctact gcctgcggtc tgttccatga catcggcaaa atgctgattc cctattccat
     3301 catcgccaag cccggcagac tgtccgagga agagtttgcc acggtaaaaa aacatcctgc
     3361 tctgggatat gaattactgc agtctcagga tgtggacgtg catgtgaaaa atgcagctct
     3421 gatgcaccac gaacgtagtg acggcagcgg ttacccctcg aaattaaaag ggaaccagat
     3481 cgatccttat gcacggatgg tagccattgc ggatgtctac gatgccatga cggcagcccg
     3541 ctgttacaga ggacctttgt gtccgttctg tgtgattgaa atgttcgaaa cggaaggctt
     3601 ccagaaatac gatgtttcca ttctgcttcc tttcctggaa aacgtggtaa attcctgcct
     3661 gcaaaaccgt tgtctgttga acgatggacg aaaagtcaca atcatttaca tcaataagga
     3721 aaaattgtcc cgccctgtag tacagtacgg cgaggaatat atcaatctgg cagaccagcc
     3781 ggatctgaaa attgaaaagc tgttgtaagt tatcatttga aaattatttt ttacgcacaa
     3841 ttttgtacct cacattgtac tataatacaa agcactaaag catatattat gtgccggtgg
     3901 cacatgttta gcacggatcg aagcaaagcg gagaaatggg cttgaaaatg cggaggtacg
     3961 gaaaggtata ggtattctat ttatgtcaga cacaattcat ttgtatgtaa atatgtccgg
     4021 aaatacaatt ccgggcactt cttttgccac catcggagaa gcattggctt ccctgccgga
     4081 tgttcctgcc tcagaatccg gaaagcagtt cccggctcca gtttcggagc tgccgccggt
     4141 gatcctgcat atcggtaaag gaatttatcg ggaaaagctg gtaatcaccc gtccgaatat
     4201 tacttttgag ggtaccggcg gtgacgagac gattcttgtc tatggagatg ctgccttcga
     4261 tctcatgccg gagggggata agcgaggtac cttccgtacc gcttccgtcc ggatcgacac
     4321 tcatgacttt accgccagaa acctgacttt ccagaacgat tccggctacg gccacaccgt
     4381 agggcaggca ctggccgtct atgtggacgg tgaccgcaat gccttttata actgccggtt
     4441 cttaggcagt caggatacga tcttcgatgc acccctgcca ttaaaagagg ctcagcccgg
     4501 cggattcaag ggtcccggag aattcaagcc ccggaccata ggacgccatt attatgaaaa
     4561 ttgctttatc cagggtgatg tggatttcat cttcggcagt ggaatcgcct ggtttgagca
     4621 ttgcacgatc ttctccaagc tgcccggaga ccggattcca ccggaaagtc cggagtctga
     4681 agtaatctac ggctatgtga ctgctgcctc caccccgcag gaagagccct acggctacgt
     4741 gttccatgaa tgcaaattag agagcgactg cccgcccaat tccatctatc tgggacgtcc
     4801 ctggagagaa tgggcaaaga cagtgttcct gcactgtgaa ctgggtgtac atatccaccc
     4861 cgccggctgg caggactgga aaaagcccca cgaccacttc tactacggag aatacaactc
     4921 ctacggcccc ggtgcctccc ctgccacccg cgccgacttc tcccaccaac tgacggatgc
     4981 ggaagctgcg gagtataccg tggaacgggt gatgaagggt tgggagccac agacattctg
     5041 aacctttgcg tgaaatgctt tttcgagaat tcaaaccgtc cgatgcggtc cattgtgaaa
     5101 ggagttccga catttatgta tggagaagaa ttgtctcgtc aactggcact ggattactgc
     5161 tgtagtcccg cagatgttgc tgattcggag aaccattttt caatttatgc tccgcaggaa
     5221 ggacgtcgcc gctttcagga ggctttgatc cgtggcttga aaattgctgt cgtcaatggg
     5281 aaattattat ttaccggttc cgaagaaatt gttgcagaat gtagaaagcg ttatgccgat
     5341 gtaaccggtg aatggttctt cgatgcgaaa agacttcggg aaatagaaga actgctgttg
     5401 ccgttccacc tccgtgttgc gcaggcgcat cctttttttc tgccggaagc cgatgtcatg
     5461 ccttcctctg gcattccttt gccagatgcg tctgatgctc tcgccttcga tttgattcgg
     5521 tatgaccaaa atgccattct gcagttccgg gaagacaacc gctttgatga ggcatttgct
     5581 ttcgatccct atgccccgga tgttctgggt attgccgcag caaaagacgg acagatcctt
     5641 ggcatggccg gtgccagtgc cgacagtcct ctgttctggc agattggtat caatgttgat
     5701 tcccatgctg tcggaatgca tgtgggttct actctggtaa gacttctggc gcaggatatc
     5761 atcgctcaag gtgccgttcc ctattacggc acaagcatgt cacatattgc ctcccaacgg
     5821 gtcgcccaca gagccggatt tgctgtggcg tgggcggaat tggttgcgga ggaaataaag
     5881 taaatggaaa gtta
//
LOCUS       DAWEJW010000129         5884 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4112_length_5884_cov_62.917482, whole genome shotgun sequence.
ACCESSION   DAWEJW010000129 DAWEJW010000000
VERSION     DAWEJW010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4112_length_5884_cov_62.917482"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1324 a         1643 c         1219 g         1698 t
ORIGIN      
        1 ggtgccacct ccttgatcat cttcgaagcg tacatttccg gctgccatgg ttatacttca
       61 tcctcatagt accggatcat gcataccagt tcgccgttct ctacggacac ctctgcttcc
      121 tctcccagga attcattgct gatcccgatg gggaaatcgt tggtgatcac ggcattttcc
      181 aaaggatatt tcatgccgcg gatattcacg ccttttacct ccttcgtcag ggcaaacagt
      241 gacatggtgc cctccagctc cttccggaaa tgcacagctt cgttctgcag taccagcacc
      301 attcccgtac cgtctaccag gtaccccacc gcgccatgat ttttgagata cagcagacac
      361 tggatatttg ccagagtatg atcaaatctc ccgccggtgg ccgcataaat acggaaatcg
      421 gtataaccca gctccagccc ccgcttgata gctgccagca tgtctgtgtc atccttacac
      481 tccggcaggc ggcagatctt ctccggaacg gtctgttgta actgttccac agcaagcttc
      541 tcctgttcgg tcatcgagtc acaatctccg ataatcagat ccggctccac atgcaggagc
      601 ccgcagtagc ttaagccgcc gtccaccgcg atcacataat cttcctccgt cacagacagt
      661 tctcccatcg tgagatctcc tgcgccgatc aaaatacatt tacccatagt atttactttt
      721 tttcctttcc tgtaacggtt ccaccttgcc attcccaaag tcacatgaat gcgcctgcgc
      781 cgctttgtga ccggccttat ttccgaaaca gacatttcgt ttcgtctgca agccgttacg
      841 caatattcta caatttctgc gtttactttt caagccatac gcagtatatt taatctgttc
      901 aaacttacaa cgatactcag aggatcaaca tccctgtatg tgaaaggcac tctcatgaat
      961 ctatatgaca atcagccgct gaccgttgcg gagctccgca ggttgcaggc tttggaaaac
     1021 cgatatcgga atgctgccag agaagtgttt acgaaccgtg tggaatacta ccacaggttc
     1081 accggtgggc actacacttc tatcaccatc cgtgaccaga agacccgctg gggaagctgt
     1141 tcctcgagag gaactttatc cttcaattac cgcctgatct acggtcccgc cggtcctctg
     1201 gattatgtgg tagtccacga gctctgccac ctgactcata tgaaccattc gaaggacttc
     1261 tggaacatgg tcgagcacat catgccggat taccggatct ataagcagtg gttaagggag
     1321 cacggacagg agcttaccct gacgacacat ctgaaaaagc agggcattcc catccgcctt
     1381 ggctaaggcg ccatgctata aatggcggcc tgtttttcaa cagaccgcca tcttttacta
     1441 ttctgctcta cgcagtgcta tcttccgttt atctgttctc gtttacatag acctgtacac
     1501 ggctctgaat gacacctgcc acatctctcg cactcttctg tcctgagaag tatgctccgg
     1561 cttcttccga aatgatattg gagattgcct cattatagta tcctctcttg ttcacgcttt
     1621 ccacaaagct tacgatcctg tccacctgct cctgtgtcag ctgcggcagc agtatttcct
     1681 ccccattgat gtaataggtc tcatcgtatt ccactttgtt gccgttctca tccgtataat
     1741 acggcttctt cgtagcatcc tgcacattct tctcaaaggt gctgcgaagt accggaagat
     1801 tgtattcctg ttcctgtatt ttatcctgat attcctgtgt cagatagtag cgcaggaatt
     1861 cccatgcacc gtccaggttc ttggattttg cagacagggc atacatcctg ttacctgccc
     1921 ataatatgga tcccatgtct ccatcggtcg ggaagcccac aaaggagata tcctcaccga
     1981 agacaccgtt gatcgtaccg ttcagatcac ggatgttgct gatggagatg ccatccagca
     2041 gggttctgtc ctcacggaac tgggattcat agttattcca gtaatcctct ccccagtaat
     2101 cctctccata ttctaccggc agcccggctg cataggtcag cattgccacg aaattatccg
     2161 tatcaaatgc acattttccg gtggatacat ccacgaaatc actgccacaa taattcatca
     2221 tggtatacag gaagctgtct ctggtctcat cactgaagag tgcggttcct tccggcatgg
     2281 tatcccggat cgtctgcagc tcttccatag taatggaggt acggtcaccg aaaatagatt
     2341 cttttcctac catagtagag atatagaagg aaggaatcac atagtacagt ttaccgtcca
     2401 cacggtacgc atcccagaca ttctgcagat agtcgttttt cgataattcc tcatcccccg
     2461 caatgagatc atccacattt gccaccagtc ccttggcaat atagctgtcc attgacatat
     2521 tgctgtccac gaccagaata tccggcattc ctccggagat gatatcatta tttaactgct
     2581 tcacaccgag agtataatcc tctgaagtgt tgtaggtatt gtattccttg actacaatac
     2641 ggtaagtgtc accggactta ttatactcta ctacttttct cttcagatca tagtcgatat
     2701 agttaccggc cagtgtaaga acgatcctgt ccttgatatc ctccggcttc acgtgggtga
     2761 acagaccgcc gcatattttg ccttcgttgt tatcatagta aaagcccagg aactgttcct
     2821 cgtccagtat caggaaattg ttcaggctgc cggcagccag atctgaattg atataactca
     2881 tgatctgctt cgactcctgt gtctgcacat tataaccaaa caatccgtta ctatcggcat
     2941 agtacaggat gtcaccgtct ccgggcacca tattataata accgccgttg gtcagtgtcg
     3001 aggaaagctt tattccttct gataccgcat ctgttgcagg atcataggtt gcgatataca
     3061 tatccgtgta attgctctca tcataataga tgatctgcag cttgccatcc ggcgtaatga
     3121 tattcgagct tgcattgttg aaaagtgttg ctgctgcctc tggcagctcc ttccggtctg
     3181 tgataccctc ttccgtcaca gtgcatttct ctattttatc accgcttaac atcagtgtca
     3241 gggtcccatc ctcattgggg atgatactat gtacccagga atagctttct tctgtattca
     3301 tatcattcag atcagcttcc cataagatag tcccatccgc actccagcag atcaatgtat
     3361 aatcattcgt actgctgtaa tcatcttcgg aatatgtctc actatagtaa ttgcgtattc
     3421 cgtagacatt tccatccgag ccgaccgtac aattgttgag cgatgtgcta gtataactgc
     3481 tgttccctgc cggtgcatct accacatcca tatccgctgc cgcaatatcc tgtgtgctgg
     3541 ccgcctgctc cgtaagaggc actgcttcat ttccgtcccc agaagcctca ccgccctgtg
     3601 caggtgccgg agtagccgga gcctctcctt ccttccacat aggcagttct acggtgccaa
     3661 tgacggtacc atcctgtttc attgtagtaa gatgcagcgt actctgactg ccgttatcgg
     3721 aatattcata ggtctcaaac agaaggtaaa ccgtatctcc ctttctggtc agcagatcaa
     3781 tattcatatt gtctgaatcc atattgagat ccagggcctg ttccttaaat acatactgtt
     3841 ttgcaagact ggcatcggcc gcactgctgc cgccgtttcc gttcccgccg ttaccctttc
     3901 cacaggcagt cagacctgcg atcattgcca cggtcaacgc gatactgatc cgttttttcc
     3961 ctttcttttt cattctccct ctccttttct tgtctgtgtc tgttgtttta cgactgctgt
     4021 tcctcttttg tctcctccgg tgtctgcgtt tcctctgtct gcatgttcat tgtctgcgct
     4081 ttctccggct cttccgaagc tgcatccgca agctctgttt cctccgccgg ttcatccgtc
     4141 agccaggaga attcactgtc tgcataaggg tcttcctcca gttcccgttc caattcctcc
     4201 aggacatccc tctccttgtg ggggtgcttt ttctctctgc ggcgttccac agcccgctct
     4261 gccttatcct taccggtatg ccatacctcc ttcaaggtcc agcccttatg gcgccaccag
     4321 tgtacaaagc accagataac ggtcactacc ggatataata acagaagcag cataactacc
     4381 gtaagcaatg caataatgtc ttcatagcct ctcgccagat tctcccagta ggggtagatg
     4441 atggcttttc cgttcatgga acgcgttccg aaggcaagga ttgttttgat ccggttgggc
     4501 agagaataac gtctgctgtt ttccacgatc tcaacgtttt tctcatctat gccgatccct
     4561 tcttttactt tgctgtaggc gaactcatcc acagggttcg gcatcacgat ctcgtaatgg
     4621 ttgattccgt tattcgtacc ataagcagac agtgtctcat aagacacata gaccaccgtg
     4681 gaattcagtc ccgctgcttt atccattctg ctgtccgggc gctgtaccac accggtgacg
     4741 atatggggaa cattgccgat atataccgtc attcccacca catcattgga gccgaacagc
     4801 tgccaggccg catcctgatc gatgatacaa tagtcctgca tcagatcgtt accggaaaaa
     4861 taggagcctg tgatcagttt taacggatgg aacataaaaa agtctccgcc gataccgatg
     4921 gcattggcat ccagcgtatt tttcccgttg accagggtga tcttaccgtc tgcgctatag
     4981 gcatccaccc agagtctggc acttggattc tcggaggtct ctgtaatcga ggcctcctgc
     5041 agcacatttt tcaatgaata ttcaaagctt tgaatggtat cctccgtcac ttctgctttg
     5101 gaggagaaaa agcagctgat ctgtgccaca tccttttttt cactccatcg tccggccatc
     5161 tgctgggcat cctgggtatt tgccagatgg ttggtccatc ccagcaaaag caggaacaga
     5221 agaaaggata ctactgcact gcagtaccat atgcatttct ttttattctg tattcgaatc
     5281 cgaaacatct gatctgtttc ttccttcctg ttaaattatt ttccgtctga aagacctttt
     5341 atgaattatt tgccagtctg accagctttc ctgcctctac cggctgggta gatgtcgtaa
     5401 tgacaaattc atagccatat aaagctccgc tcactgcgga ctgggtgtca tcacttgcca
     5461 gtacctccac atccacacgg gtcgccacat aacggttacc taaggggctg ctcttggatt
     5521 ccacgatcag gataaactta ccgttactgt cctcacggat ggcactgttg ggtacgatca
     5581 gatcgtaatt ggcactcttc tgtcccacgg atacatttaa ggactgtccg ggtgtcactt
     5641 catctccggt gatatcaaag gtcagcagct tcttctgtcc gggatctgtg gtatccggct
     5701 tgatgctggc cagtgtaata tccatattac tgtatctcca ggagttcacc aggtctgcct
     5761 tatctcctac ggaaagtctc ttcgcctgat cattggtcac ggaaaaactc atggtaaagc
     5821 ccttaccctc cggctgcatg gtcaccagtg cagtatccgg ctgtgcctcg tctcctgcct
     5881 tgac
//
LOCUS       DAWEJW010000130         5841 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4140_length_5841_cov_56.614414, whole genome shotgun sequence.
ACCESSION   DAWEJW010000130 DAWEJW010000000
VERSION     DAWEJW010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4140_length_5841_cov_56.614414"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1672 a         1204 c         1567 g         1398 t
ORIGIN      
        1 ctccgggagt tctgtttgta tttaaaacgc caatttctgc gaaaaaaata caaaaacatg
       61 agattttgct atgtaaaaag tctctatttt cgctacataa gcaaaataaa gaaattccgt
      121 acaaaaaatc ctggtttaga aagttttttg tacggaattc attgttttga ggctttttac
      181 accaaactaa ctctccagtt acaacaagga aaaagatctt tggctcggaa ataacgaaaa
      241 ttcaaataag cagataacgg gaatcgaacc cgcctttcca gcttgggaag ctagcgttct
      301 accgatgaac catatctgca aatggaacaa gaaatattat aactgatatt gtttcctttt
      361 tccagtattt tttgaaaaaa gggaacaaca atgctaaaag agtttgcatc gaaagagcag
      421 acatgggatt cctctggcag aggttcttca ttttccagac gggatgctac agccggttca
      481 agggcatcat aagccatggt ataggcagct tgaatgtctt ctccctgcgt catgcattct
      541 ggaaaatcag gaaaggtaat ccaaaaccca ccttcttcag ctttgtgaat taatgcggaa
      601 taaaaaagta tattcaaagt atattcatag atatcctcat ctaaacgtgg aacgggtgtg
      661 ataattaacg gtgtcatggg taatcacaaa attattagta agaaaaaaca gaatatttca
      721 gaatagcatc agccgccgag tgttctacgg gatattcaat aaggtgaacc tagacactgg
      781 cgatacgggc gaaccgcact cctcccggaa ctgatgccaa ggttattata taacgatacg
      841 cagaaggaca caatgggaag tgtcagtttt gcgtattttt ttatgggttg acaataatcc
      901 gatttcggat tataataccg tttagtccga tttcggataa aaacaaacag atgcaacgat
      961 gtagttacag gaggaaagcg atatggaaga ggaaagcaga aaatcttttg cgctgatcac
     1021 attgtacaat cagctgatca aagagatgga tgacctgtac cgggtctatg caaaaaactg
     1081 taatctgtcg gagacggctt tctggattct gtattgtatc cgggaacggg aaaaagaggc
     1141 gtttacccag agggagatct gcgactattg gttctacacg ccccagacag tgaattccgc
     1201 actgaaaaat atgacggagg aaggtctgct tattctgcgg acggaggaag acaaccgcaa
     1261 gaataagagg atatatctca cagagaaggg gaaagagact gtggagagga tcgtggttcc
     1321 gctgatggat gcggagcgcc gggcattggc ggcgatagga gagcaggagg cagaaatatt
     1381 cctgaaaact atgaaaaaac atacggaatt tttccgggaa gaaatagagg aactgacaag
     1441 acaaggggat cccgcgtgat cgcaggaacc tccgatgatg acacgaaaac gatgcctgca
     1501 taattcgcag aagtatctgt tgagaaagtg ccggaaggca tgcgggtaat agtaagacat
     1561 gaggttatta tgaaaataca gttatcagat cattttagtt ataagcggct gctgcgcttc
     1621 gtggcaccgt ccattttaat gctgctatgt acttcggtgt acagcatcgt agacggtttc
     1681 tttgtatcca attttgtggg aaagacacct tttgccgctc tgaatctggt gatgccggtg
     1741 ctgatgggag taggaaccat cggattcatg gcgggtaccg gtggaagcgc cgtggtctcc
     1801 atgactctgg gagaaggtaa aaagaagctt gccaacgaat atttttccct gatcgtatat
     1861 gtgacgctgg cggtatccat cgtggtggca ctgatctgtt ttgcactggc gccacagatc
     1921 gctctggcac tgggggctga cggagaactg gaagcggatt gtgtgcgtta ttcccggatt
     1981 ctgtttttat ccacaccggc atttgttatg cagtatctgt tccagagctt tttcattgca
     2041 gcggagaagc cgacactgag tctaaaggta aatgtgatcg cgggactgac caacgcggtg
     2101 ctagactatg tgctgatcgt ggtatttcct ttgggactgg tgggagcagc actggctacc
     2161 gcagcgggac agctcgtcgg cggaatcatc cctgtaatct attttttcag agagaacggc
     2221 agcttactgc agctagggaa ggcgaagtgg catgggaaag taatccggca gactgtgacc
     2281 aacggatctt ctgagctggt gacaaatatc tctacttcta tcgttagcat cctgtataac
     2341 ttccagctga tggaagcagc aggcgagaat ggtgtggcgg cttacggcat catcatgtac
     2401 gtggacatta ttttcatgac cttgtatctg ggatattccc tgggctctgc tcctatcgtc
     2461 agcttccatt acggtgcggg caatcatgcg gaactgaaaa atctttgccg gaaaagtctg
     2521 gtcattatct cagcctgcgg tgtgattctg ctgaccgctt ctcagattct ggccggaccg
     2581 ttagtaaaaa tcttcgcaaa ctacgatcag gagttattgg agatgaccac ctggggattt
     2641 cggatctatg ccattgcttt catggtccgc ggcatgaacg tgtggggttc ctccttcttc
     2701 acagccctga acaacggagc cgtatccgca gcaatctctt tcctgcgtac cttcgtgttc
     2761 cagatcgtca tcgtgctgat cctgccgaaa ctgatcggca ttaccggtgt atggctgtcc
     2821 atcgtactgg cagagttcct ggcactgttt gtgacaatag gattcctgat cgccaagaga
     2881 aaaaaatatc attatgcata actattcaga gccatgcaaa ctgcgttctg cgaatgcttc
     2941 ggattgtttc cttgacagaa aaatgacaaa tacttatact gaaagtgcac tttgttctct
     3001 ataaaagcgc agaaacgagg aaatgaaaat gggtgtttta tcgaacttag aaccgaagaa
     3061 agtattttac tattttgaag agatcacgaa gatccctcac ggctccggca atgtggagca
     3121 gatcagcgat ttcctggtgg actttgcgaa agcacataag cttttctata tacaggatgc
     3181 tatgaaaaat gtcattatgg tcaaggaggc aactcccgga tacgagaatg aaccggtggt
     3241 gatcctgcag ggacatatgg acatggtagc ggtgcagaca ccggattgca ccatggatat
     3301 gaagacggaa ggactgaagg taggaatcga cggagacagc gtctatgcgg agaataccag
     3361 cctcggcggc gatgatggta ttgctgtggc ttatgcactg gcattattgg attccgagga
     3421 catcaggcat ccccgtttgg aagtcatcat tacggtggat gaggaagtcg gtatggacgg
     3481 agccagagag attgacctgt ctatgttaca gggtcatcgc atgatcaatc tggattccga
     3541 ggacgaaggc attttcctga ccagctgtgc tggcggtgcc agagtgaact gccgtttccc
     3601 gatgaaaaaa caggagctta cgggcgtgcg ctatgaggtg gaagtctccg gcctcttagg
     3661 aggacattcc ggcggagaga tacataagga aaggggcaat tccaattgta tcctgggaag
     3721 acttctgtgg aagctgtctg agaaaatgcc tgtaggactg gtatctgcgg acggaggact
     3781 ggcagacaat gccatcccca gacagacgaa agcagtagtc gtagtagagg agaaagatgc
     3841 ggaaagctta gagcagcagg tagcagcttt ggcagcagaa ctgggtgacg aacttgccac
     3901 caaggatccg gacgtaaagg taaccgcaaa aaggttagca gacactgcag agacaatcac
     3961 ctgtgctgcg ccggaagcta caaaacgtgt ggcagccttt ttgcaggcga ctcccaacgg
     4021 cgtacaggcg atgagcgcag atatgccggg gctggtacag acttccctga atctgggtat
     4081 cctgaaagca gatgtggaag ttttacgggc agagtattct gtcagaagca gcgtggaaag
     4141 tgagaaagat aacctgatcg caaagctttc tgcactggtg gaactgacag gcggcgatta
     4201 cgtggtgacc ggagattatc ccggctggaa ataccgtgtg cattctccac tgcgggaaaa
     4261 aatggtgaag ctgtatgcgg aaatgtacgg cactgagccc aaggtggagg ctatccatgc
     4321 aggactggag tgcggactgt taggcagcaa gatcacagac cttgattgtg tatccatggg
     4381 accggatatg aaggacattc atacgaccga ggagacctta agcatatctt ccacgaagag
     4441 agtgtgggaa tatctggtaa gggttctgga gacgaaggac tgactgaatt tgagtaacag
     4501 gaagtccaga gaagccaata agaataagat agaaacgatc gaaaaatgta aagagtttgc
     4561 aaattgaaaa agctaaaata taaataacgt tggaggaaat acactgtcac ggcggtgtca
     4621 ataaaaaaat gagcgataag attttattcc agtgtgacta taatgaggga gcgcatccga
     4681 aggtgctgga gcgcctggtg cagaccaata tggagcagac accgggctac agtgaggata
     4741 aatactgcga agaggcgcgc aagaagatcc gtaaggcctg tggggatgac agtctggcag
     4801 tccatttcct ggtaggcgga acccagacca atgtcaccgt gatcaatgca gcacttcgca
     4861 agcaccaggg cgtgctctgc gctgtcacag gacatatcaa tgtccatgag accggtgcgg
     4921 tggaagcctg cggacataag gtactgggac tagaaagccc cgacggtaag atcacggcag
     4981 cacaggtagc agagacttac gaagcccata ttcacaacga cagctttgaa cacatggtac
     5041 agccgaaaat ggtttatatt tcaaatccca cagaggttgg aaccatttac agcaaagcgg
     5101 aactgaccgc tctgtccgag acctgtcaca aatacggact gtatctgttc ttagatggcg
     5161 ccagactggg ctacggactg gcagcgccgg acaacgatct gacattaccg gagatcgcgg
     5221 cgctttgtga tgtcttttac atcggtggaa ccaaggtcgg cgcattgttc ggagaagcag
     5281 tagtgatcaa aaatccggag cttgcacagg atttccgata tctcatcaag cagaacggcg
     5341 gcatgcttgc caagggaaga ttgctgggac tgcagtttga tgcgcttttc acagatggtc
     5401 tctatcagga gatctctgcc catgccattg ccatggcgga gaagctgcgg gaagcattta
     5461 cggcgaaggg ctacaactat ctggcaccca accggacgaa ccagatcttc gtcattgtac
     5521 cggatgcaca cctggcgaag atttcagaac agttcgagta cagctatgac cagcggatcg
     5581 atgccacaca cagctgcgtg cgattctgta ccggctgggc cacgaaggaa gaaaatgtgg
     5641 atgcactgat ccggtgcgtg gaaaaattat aatatgtttg gaagattccg caagccggga
     5701 aaggtttgcg gaattttaaa aatcagtaat caatgaggta tagaacagag gagaaaacac
     5761 atggataaag aaatgaccaa tgccattcag ttaagcatca tttcagtggt tattgggcat
     5821 tgtttgttag tttaacgatt a
//
LOCUS       DAWEJW010000131         5832 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4148_length_5832_cov_51.875368, whole genome shotgun sequence.
ACCESSION   DAWEJW010000131 DAWEJW010000000
VERSION     DAWEJW010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4148_length_5832_cov_51.875368"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1797 a         1093 c         1628 g         1314 t
ORIGIN      
        1 tggcataaag aacaaaagca gcgggaaggg cacaggagta agtatggata ctaagatatt
       61 acttgaaaac ggaacaaatg aattggagat tctggagttt accctggcag gtaattccta
      121 cggaattaat gttgccaaga tcaaagagat catcacctat cagccagtaa caccggttcc
      181 gaattcacat cccagtattg aaggtatttt catgccgaga gatactatga tcaccgccat
      241 tgatctgaag aattgtctgg ggcgtggtga atctgagaaa aaaggattat ttatcgttac
      301 caactttaat aagctggata ttgcattcca tgtagaatct gtattaggta ttcacagagt
      361 aagctggaga gatattatta agccggatat caccatttct gcagcagatg agagtgttgc
      421 taccggcatt atcaagaaaa atgacaagct gatcattatt ctggatttcg agaagatcgt
      481 atctgacatc aatccggaga caggactgaa gatgtccgag ctgaatgaac tgggagagcg
      541 tactagaagc tctgttccta ttctgattgc agaggattcc cccctgctta acaagctgat
      601 tgtagattcc ctgaaggcag ccggctatgt aaatctgatc catacagaga acggtcagaa
      661 agcatatgat gttattacgc agtgcaaaga ggatggtaca ctggatcagc atgttcgttg
      721 tatcatcacc gatattgaga tgccggaaat ggatggtcac agactgacca agcttgtgaa
      781 gagcgatgat gcgaccaagg acattcctat tatcattttc tcatcactgg ttaatgatga
      841 catgaagaga aagggagagg ctttgggagc agatgcacag ctttccaaac ccgagatcgg
      901 taatctggta agaattgtgg acgaactggt agcagagaag catttagaca gatagtaagt
      961 ttagagaagc aaaaaggctg ggatagtccc agccttttgc tataatatca ggatacagtt
     1021 atggataaac aaacagaaga aattcttcag aaaattgaag aagcggaata tgtgctggtg
     1081 ggcctgggac aggagtttga catggaattt ttcctgcaaa gcagagaaga ttaccgcaga
     1141 atccgggaac agttacaaca gcagaatgta ctttggcttt tgccttatgt ggaagaacaa
     1201 ttccgcagac agtatttgcc ggagatggaa gcagagatcg agaagggatt gcagaagctg
     1261 gcaaaagtat tggaaaaaaa gagttatttt gtggtttcat cttcgctgaa tcataaattg
     1321 gcagaggttc cctggaaaaa aatgttattg aaaaaagagc gattcgtggc accctgtgga
     1381 gactggacga aaaaacagtg cccggacggg tgtgaggaag gcatccagac agtgacagaa
     1441 gcagatgagg aacaattgca ggagagtttt aagaagctgc agactaacgg gttttcggtt
     1501 ccggatttgg gaaaatgtcc aaagtgcggg aaaaagttgg tactcaacaa tgtctatgcc
     1561 ggccgatacg atgaaaaagg atatttgaaa acctggacag aatatcagaa ctggctgcaa
     1621 aataccctga atcataaaat ggtgttgttg gagattgggg aggggaaccg ttttcctacg
     1681 attatacgtt ccccttttga gagaatcgct ttgtttcaac aaaaagcaga cctttattgt
     1741 atagatggag aacaaaatcc cattgcctgg ctgctcagcc tgtgttaaac ttaacatgct
     1801 agtaacttgt gagaaagcgg aggaatgaca tatgccttca caggaggaat acctggataa
     1861 tttattaaaa ggaattgaaa cagaaaatac ggaaacagag gttacagagg agccgaaaat
     1921 gaccgggacg gaggaagaaa ctgccaaaac accggaaacg ccggagcctg tggaagagag
     1981 tggaactcag gtcgatatga gcgatatgga tgatctgctg cagtcggcgc tggatgctca
     2041 aaaagctgat tcatcagagc aggaaacgga agcaagtcag cctgtagcgg aaaatacagc
     2101 cattcttcca gaggagacag cttctatgtc tgaggatgaa attaacaggc tattgcagca
     2161 gagccaggag caatccgggg atgctccggc acaggagagc aacgatgcta atgacgatct
     2221 gatcaaaatg ttggagaatg cggatgacga gggattgtca aatattctgg atcaggtgac
     2281 accggagact gcacagccgg aggaaacagc agagcgggaa gaagacaacg gcaagcgcgg
     2341 aaaaaagaaa aaagaaaaga aaaagaagga aaagaagctg ggattttttg aacggtttaa
     2401 gaagaaatcc aaggaagaac cggaagcagc accggatgag acagaatcag cacagcctgc
     2461 gggagaggaa gttacatcgg atatcccgga aactgtgccg gatgatcttg cggatctgtc
     2521 ttccctgaag aatgacatgg atgatacaga caaaacaccg atggaggctt ctgacaacac
     2581 ggaaagtgta gaaagtacgg aaaatgctgc ggaaaacgga gatgcaggag gcctggatga
     2641 cgatctggac gctttactgg cgggagcatt tccggagcag tcggagaaca gcacggataa
     2701 tgcatccgga gaaccggaat ctgcagatgt gatggatatt ctgaaggctg caggcgcaga
     2761 catcatggat gagccggaac agaagaaaga gaaaaaaggc ttttttgcca aattattaga
     2821 tctattcacc gaggaggacg aggaagaaga ggaagcggat cagttgcagc tgtccgatga
     2881 gaacaagaag atcctggaag agatggacaa gggcggcaag aacaagaagg gaaaaaaaca
     2941 gaaaaagcct aaaaaagaaa agcagcccaa gcccaagaaa ccggcaaaag aaaaaaagaa
     3001 aaaagaaaag ccggaaaagg aagataagcc gtcagtatcg gagaaaaagc tttctccgaa
     3061 gaaaatcctc ccgattgtag tggtatgtat ttctctgggg gcggtgattc tgttgctggg
     3121 taacttcctg gcggattata ccgcgaagcg cagcggtaga gaagcttact atgccggaga
     3181 ttatcagacc tgttaccaga atctgatggg caaggaactg gatgagacag aacaggtgat
     3241 gtacagtcag tccgagagta ttctgaccat ccgtatgtgg ctgcgggagt acgaagtatt
     3301 cgtaaacgaa ggctctgaat tggaggcatt ggacagcctg cttcaggcag tcagggatta
     3361 tccgacattg ttaaattatg cgacacagtg gaatgcgcag gatgaggtaa gtctggcatt
     3421 ccaggatatt ttgaatatcc tttcccagaa atatcagtta agtcaggaag aggcacaaca
     3481 gattgcggat atttccgata atgtagaata tacgaagaat gtaatgttgg ttctgcaaag
     3541 gctgggactg ggattctggg agttcccgca gggaacggta acggaggaaa catctgcacc
     3601 taatacggag gaggcgcccg cacagctgcc ggatcctctc cccgaggaaa aggagattca
     3661 gcaatgatca cagtgctcga ctatgatgct ggcaacataa agagtgtaga aaatgcattg
     3721 aattttctgg gagaagaggt aaagattacc agagacagag aggaaatcct gtccgcagac
     3781 ggtgtgatcc tgccgggtgt gggagcgttc ggtgatgcca tggaaaaact gcaccagtac
     3841 ggactggtgg atgtcattca tgaagtagtg gacaggcaga tcccttttct gggaatctgt
     3901 ctgggcttac agctgatgtt tgagagtagt gaggagacac cgggagtaga gggactgcac
     3961 ctgttagacg gtaagatccg aaggattcct gcggcagaag aactgaagat tccgcatatt
     4021 ggctggaatg accttacttt tcccaatgaa ggaagacttt tccggggcgt ggaagagcat
     4081 tcctatgtat attttgtaca ttcctattat ctggaggcag cagatccttc cattgttact
     4141 gccaccacag aatatggaac tttgattcat gcttccgtgg aaaagggtaa tatttttgcc
     4201 tgtcagttcc atcccgagaa aagttcccgg gtgggaatga aaattctgca gaatttcata
     4261 gagatcacac aagagttccg ggaaaaagat aagcagggag gaagatgata tgcataccaa
     4321 aagagtaatt ccctgtctgg atgtgaagga tggcagagta gtaaaaggtg tgaattttgt
     4381 gaatttccgg gatgccggag atccgtcgga ggtagcatct gcttatgatg cagccggtgc
     4441 ggacgaagtg gtatttttgg acattacggc ttctgcagac agtagagcaa cgcagttaga
     4501 gtgggtgcgg aaggtagcca gcaaggtatt tattcctttc actgtaggcg gaggaatccg
     4561 taccgtggat gattttaagg cgatattgag agaaggagca gacaagattt ctgtgaattc
     4621 cgcagccatt atgaatccgc agctgattag tgatgcagca gagaaatttg gcagtcagtg
     4681 tgtggtggtc gctatcgatg ccaagaagcg ggcagacggc ggtggttgga atatttataa
     4741 aaacggtggt cgtgtggata tgggcatgga cgctgtggag tgggccagaa ccgcagagaa
     4801 gatgggagcc ggagagattc tgctgaccag catggacggt gacggcacga aggaaggcta
     4861 tgatctggag ttgaccaggg ctgtagcaga gagtgtaagc attccggtca ttgcatccgg
     4921 cggtgctgga cagctgtctc atttctatga tgctctgaca gagggaaagg cagaggcagt
     4981 tctggcggca tctctgttcc attacaaaga attagagatc aggcaggtga aagaatacct
     5041 gcgggaaaaa ggaatcagtg tccgcctcta gggacgacag ggaggatttg actatggcaa
     5101 tgatcacgaa tgactggctg acggctttgg gcggagaatt ccacaagcct tattacagac
     5161 agctttttga atttgtcaaa aatgaataca acaccaccgt ggtcttccca ccggcggatg
     5221 atattttcaa tgcatttcat ctgacgccgt taagcaaggt aaaagtggtg attttaggac
     5281 aggatcctta tcataatgtg ggacaggctc atggactgtg tttctcggtg aagccggatg
     5341 tagagattcc cccttctctg gtgaatattt atcaggaact tcacgatgat ctgggatgct
     5401 atatccctaa caatggttat cttgtaaaat gggccgagca gggtgtcctg atgctcaata
     5461 cggtgcttac cgtgcgggca cacatggcca attcccatca cgggaaaggt tgggaagagt
     5521 ttacagatgc cgccatccgg acactgaatg cacaggatcg tcccattgtg tttatcctgt
     5581 ggggcagacc tgcccagatg aagaaagcta tgttgaacaa tcccaatcat ctgatcttag
     5641 aggcccctca tcccagcccc ctgtctgcct accgcggttt cttcggcagc agacctttca
     5701 gcaagactaa caacttcttg aaagaacacg gcgtggagcc catcgactgg cagattgaaa
     5761 atgtgtaaat tgtgagttac gagccatgca aaaatagagt ggaacacctc gatgggcgct
     5821 tgcgcacaag cg
//
LOCUS       DAWEJW010000132         5804 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4170_length_5804_cov_39.536963, whole genome shotgun sequence.
ACCESSION   DAWEJW010000132 DAWEJW010000000
VERSION     DAWEJW010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4170_length_5804_cov_39.536963"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1382 a         1625 c         1043 g         1754 t
ORIGIN      
        1 ctgtccggaa ggcaaaaata agctgtgcta gcaatatttt gtctgcaagc acagccttgt
       61 caccaaattt ttcctattgt gtttttagaa tctatctctt ttccccattt tcaaagtaaa
      121 tccaatatta tttgggcatt tccgagggaa tgttcagata aacatcctct tttaagtgaa
      181 atccgcttcc gatgatcaca tcattcatat tgtttttatc cacactgatc ggttccagac
      241 caatgtacgg aacttcatat tttccattct ctatggttac cgtatctcct tccggcaacg
      301 gctctccctt tgccagaagc accgcacact ccgctgcctt ctgcgacatt ttttccaccg
      361 gtttgtagac cgtcatgacc tgtgtcccct cgacaatgcg ctggcacgct tccaggtcgg
      421 catcctgccc cactaccata atatctcctg ccagtctctt ttctttcagg gcatgtacca
      481 ctttgcttgc aagatcatca ttgccgcaca taatggcatc cgcttccgag acaatatcca
      541 tatgatcata gatatacgtc cccgccagtt ctgctttcca accatccgca tgcatagaat
      601 ctaagatcgt cacctgattg tcctccatga ccttgcgaaa gcccttttcc accatagcaa
      661 cgttgctgtc cacggcagag cctcccagca tcagcacatt tccgccggcc agaccgtttt
      721 ccaccagggc ctctcccatc atggtaccta ctctttcgtt gtcaaacgat atatacaggt
      781 caatatctgt attctgcagc agtctgtcat aagcaatgat cttaatgccg gcatctttcg
      841 ccctctgcac ttcctctgtt aatcctttgg agtcaatgca tatcacaacg atcacgtcca
      901 ttcctttgtc gatgaagtaa ttgatctgct ttttctgctg ctccagatca ccgttcgcat
      961 tctggacatt tacttccgct cccaactcct ttgccgttga gacgaaaatg tcacgatccc
     1021 tctgccatct ttcaatgaca aaagaatcaa agctcatgcc gatctgaatt ttatcatttt
     1081 ccgcgacttc tccctgatct tttccggttt ccagatttcc gcaggcacaa agcaataaca
     1141 tcatccccag aagccacgca acacatatcc gtctcttttt cttatacact gccctgttcc
     1201 tttccctttc cccggttttc cttatactct gtcggcgtca cacccacatt tttcttaaaa
     1261 ctgcggctga aatagttggg atccgaatat ccggttttgg cacagatttc cttcatggag
     1321 cactccgtcg tctgcagtag ttcctttgcc ttgtccatcc ttaaattggt caggtactcg
     1381 ataaagtttt ccccggtagt ttctttgaat agtttactga aataatacgg gctgatattt
     1441 acctctctgg atacatcatc cagggaaata tctttgtgat agttattctt aatataattc
     1501 tttgctgtct cgataatact gcccgacttt tcttcccttt tattcagaac attccggcag
     1561 gcttccttca ctttttccag gaaccatgtt ttcatggccg ccggttccgc catttccatc
     1621 acctgcggca gatactctgc acgggaattg agctgataga tcataccgcc tttttcatag
     1681 gcaatgtgct ctgaccacag ggcaaattcc agcaccttaa gcctcacatt catcagatcg
     1741 gaaccactct ccaccatcca gtcaaaaaat gccgatgccg atgcggaagc attgtcaatg
     1801 tcgccgtcct cgacttccgc aaaaagtttt ttttccagct tcaccggata atttccaccg
     1861 tattcgcatt tgatcggcag gtcatccaca tgtgccacac tgccggtggt cgccaccagt
     1921 gcattcagcg cctccgtata agattccgaa gccatcagaa aatctttcgg ttctccgatg
     1981 ccgatgcgga agctgatatc cgttctcttg cgcagatatc tggtgagttc tctggctttt
     2041 tcaatgagtt cgatacgctc attatagtcc atcacatctt tctcataagg taccagcact
     2101 gccagtttgt ttgccatgac ggtacccaca atacagtcaa aattttcttt cagacagtcc
     2161 ctgacctcct gataatgctt ctgcattctg acactgcttc ccaccgcatt cgtcatgtgg
     2221 tttccctgct gttcatctcc gcataccaca gcaatcatgt aggcatattg cagcgtgatt
     2281 cctaaaattg accggtaatt ctcaatatcc tcgcggaaat gctcctgtaa taaaatattg
     2341 tggatcagac cgctttcaat aatcggtacg actgtctcca gtttttcctt aatcaacaac
     2401 tcatttttgc gtttcaggcg ctctgcatcg attcgctcca ttgccttctg caatgctgca
     2461 atgatccggg tcttctccat cggcttggta atgtactcca tgactcccag tttgatagct
     2521 tctttcgcat aatcaaattt gtcataagct gacattacga taaacactgt atgattattg
     2581 cttctccgga tctctttcat agcatcgatg ccgttgattc ccggcatctg gatatccatg
     2641 accgcaacgt ccggacggaa gttctctgcc agttcaatca cacttctgcc ggtcttggca
     2701 tactgcacct cacaggtatc cttgaattct ttttcgatga tgaatttcag ggaatcgatc
     2761 acgattcctt cgtcatccgc cagcatgatc ttatacatcc gtcttttccc tctcttttat
     2821 cggaagatac aggattactt ccgttccttg atttttcccg tcacttaaaa tcgtcattac
     2881 atcttcgttt tgcgtgaaaa gttttaatct tgcgataatg ttccccattg caataccatt
     2941 ggaaccgccg cctgactgct cttcgtgata attgccgcca agtacttttt ctatcgtatc
     3001 ccggtccatt ccgatgccgt tatcccggat actgatcccc agcgtatctc catccacatg
     3061 gtataccttc agccggatct ttccctctcc gttcatctct ctgatcccgt gatttacgga
     3121 attttccacg atcggctgta agatcatgct tggcatgcag ctgtccagca gatcctcctc
     3181 aatttctttc ccataatgga tatccccgga aaagcggaca ttcagaatat aaatgtaatg
     3241 atccaccagt tccagctctt ctctgaccgt cacgatatca ttactctttt ttacattata
     3301 gcggaaaaat tccgccacat tctgtacata ttcataggtc cggtcggcgc cttccatcat
     3361 ggccagctgt gctcccgcat ttaacgtatt aaataagaag tggggattga tctgcgcctg
     3421 cagatacttt agctgtgcat cctttaagtg tgcttccatc aaaagctctt tttccattaa
     3481 ggaacgccgg atttccatgc tctcgcgcag gctttccaga tactgtctga tactgataag
     3541 catctggtta aatgctcccg ttaccacgcc gatctcatcc tccgactgta cttgtaacag
     3601 ttcggtatca aagtttccgc ccgctacttc gtcagcggtt accgccagat cttttaacgg
     3661 actgatgatc tccccggtaa actttactac gatagataca ctgaccacaa tgacgatgga
     3721 taatgcaatc acactgacca tttcgaaatt acggaaagca gccgacagac ggctgtagtt
     3781 ctcggagttg ctgcggaact gttcgttgtt cagactgtaa atataggtat tgatatattc
     3841 atacatctgt gtcgcattct cgtaccggac tcggtatttc tctacatttc tcccacgctt
     3901 tgcctcaatc gtctgactta cgaggtccag gtattcttcc gacatatgcc ggatactgcg
     3961 ttccattctc tgaaaggtgg ctcccgtcac ctcatcgctc atatcctgca ccagttggga
     4021 atatgtctgt tcattccggt aaaattcctc cagagaatca ctggtcttgg aattcagata
     4081 atccgtcata ctgtcctgca ccgccatcag tgcatcagat acttcgttca gccgcagatt
     4141 cccctgatag accttatcga tctccgaaga catactgttg attcccagta gcaatatcac
     4201 cgtcactgca aatactacca gatgcgccaa tacataaatg ctgctcatct tcgcctgaag
     4261 agacagctgt cgaaattttt tcctcatacg tacctcactc tgtctcttcc tgcgcttcta
     4321 aatattcacc cacgttattc tgatcaataa ttgtgacatc cgccgtaaaa tactggctgg
     4381 tatagccaaa acgatagtac tcctgcaaag catccacgca gaaagctccc atctgctccg
     4441 tgtctatcga gatcgttgca ttgactacat ttcgctcgat tgctttgagt atcgtatcag
     4501 tatcataata ccctaaaatt gcgacctcgc ccacgcagtt gtaatccact accgcctggt
     4561 agacacaggt agtactcagt tcgttcaggc agataatgat atccggcagt ttttcattca
     4621 taaaaatatc ccggatagat tcctccacgg aaaaagtatt cgtatcgtcc actgtaatta
     4681 aggaaagttc tatctccggt ccccggattt tctcctgttc cagtgcgtcc tgaatcccgg
     4741 agcagacaat attttgtgcc gagttcagtg catgggcatt caccagtacc gctaccgtca
     4801 tgggcgcggt actctcactg acagaagcca gcttcaatac ctgtcgtccg tattccctgc
     4861 ccagattata gcttcctaca ccgacaaagc tgcagcgtgc actgtgcgta ttgtcaccat
     4921 acagcgtcac cacagggatg ccccgttcta ctgcttcgtc gatcatctgt gacatctcgt
     4981 cgctttcatc cgattctaca aaaataccgt ctacaccgga ggacatggcg atccgcataa
     5041 gctctgccct cgtatactcc tgagacagat gctcgcccag cagatccaca tatacattct
     5101 cttcctgtcc gcgttcaaaa gctccgcggt atacggactg ccagaaatcc gatttgcggt
     5161 cttctgtgat catcacataa taccggtcat atttttctct gtctgttact ttatgaaatc
     5221 cccgcacata ggaacgaaag attactgtgc atatcgaaat gactatgata gcaaaagcca
     5281 taaacccaat gacgatccat tgtcccttat tacggcctgc tctgtcagta gtacggctca
     5341 tgtttcccct ctctctcgca gaaatccgat tgcagactcc gcccaatata gataaccata
     5401 ccataataac gtggcttttt ccataagacc acaaaaaaat cctcttgttt tgacaaaaat
     5461 gcactaaaaa atgctacctg ccatagttat ccccattttc atgcatgcaa atatttatga
     5521 tttgtccaca aaaaatacgg aaaggaaact atcccttccg tattcttaca atgtgcctta
     5581 cgctacgcgg cacactttac agtcttccgt ataattttgc catcctataa atctttttcg
     5641 caagcttcgg atccaaagca tccttatcca tacgccattt ccagttgcat cccagagtgg
     5701 agggagtgtt gatcctggcg ctactgtcca gctccagata atcctgcacg ggaatgattg
     5761 cggtatccgc acagctggac aatgctgccc ggatgatctc aaag
//
LOCUS       DAWEJW010000133         5753 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4204_length_5753_cov_51.995437, whole genome shotgun sequence.
ACCESSION   DAWEJW010000133 DAWEJW010000000
VERSION     DAWEJW010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4204_length_5753_cov_51.995437"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1966..2065
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1729 a         1117 c         1512 g         1295 t
ORIGIN      
        1 acaccggtgc cttcaaattc gtactataac aattagaact accaggtaac aaacaacaca
       61 caataaaccc ttgctacact tacgaaaaaa cttaataatg tatgaactgc agaaatacaa
      121 agtacatatt acttgcagca ggaaccactg ggggtgggga tacaacaatg tattcagaaa
      181 gtatgctaaa acctgcataa gggaaaacga agggaaaaat aaaatatact gcaattgtca
      241 cagtgggtgg gacaaaaatc ttcacgtgaa agtataaggg gaaatctttc aggaaaaaga
      301 tacagctgaa cgccttacag cgaatggaca tttatgtcag gggtgacaac aagcaggatg
      361 agaaagagag aacaaaagat gaagcgaata atgtgggcaa atcccatgat caagtctgcc
      421 gggtacgatg cccagcagca aatgttggaa atcgaatttg caaacaacgg acagatctgg
      481 cagtttgata atgtaccgga ggatatctgg tatcgctttc ggagtcacca ttttccggaa
      541 ctctttttcc ataatttcat tatgggacat tatccggaac gaagaatgca ggaaattggg
      601 tagggaaccc aggcatacgg ggaatacaaa caggggaatg aaattataac gcaggaagcc
      661 gcagagatag caatatctcc tgcggcttcc tgcgttacaa aataacattt tacataaatc
      721 tctttcccaa aaaagtggaa tatgctatac tgttacaaaa gtcttgtctg atggaggctg
      781 agattttcga aataggaaga gagacgttaa cagattcatg gcaaaagaac tgcaacagac
      841 attgccggaa gcccggcgaa aaagagtgca gcggttgaag aagatgatca taggtatcgt
      901 ggcggcggga atcgtgattc cgctcctgtg ctgtgtgtat ctgatctgca ggctgcatac
      961 gattgacgga actttgaaga cactggagca gagactggat gaactggata tccggaatca
     1021 ggagttacag acgctgctgc agcagttgca ggagcagaag agtacaccgg tacagacacc
     1081 tcagatacag gaggacagtc ccggcgcaga agcttcagag ccggtggcgg aggaaagctt
     1141 cgggcaggaa gaaattcaca aggtgtatct gacgttcgat gatggaccca gtatttatac
     1201 gaatgatatc ctggatattt tagacagcta caatgtcaaa gccacatttt ttgtggtggg
     1261 aaaagaagga accaatgcag aagaggcgct gcaaaggatc gttgacgaag gacatacgct
     1321 gggaatgcat tcatacagtc ataaatataa ggaattgtac gaatcaatgg acagttttac
     1381 acaggatttt gcccggatca gggactccat ctatcaggcc accggagtgg agagtgtctg
     1441 ttaccggttc ccgggtggaa gctccaatac ggtcagcgag atcgatatgc atgattttat
     1501 cgattatctg gacagtcagg gagtggaata ctacgactgg aatgtgtcct ccggagacgg
     1561 cggaagcatg aagttatcca cggatacatt actggaaaac tgtaccaagg atatcgatac
     1621 cagagacacg tctatcatgc tgttgcatga ttccgcggag aagccgacta cagtggaagc
     1681 tttgccggat atcatagaga atattctggc ccggccggat acagtgatcc tgccgattac
     1741 ggaaaatacc aggccgattc atcatataga gtaggaatac ctgtaagaag agaatgcctg
     1801 ttgcggtttt gatcgttcgc agaacgcgaa aaccgctgat aaataacaag aaacaaatgg
     1861 aggtacataa tcatgggatt tttctcagac ttgaaggaag atctgtccca ggcggtcaat
     1921 gaactgatgc cggaggaaga gctggatcag gcagccgtgg aagagnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnacacc aaatgacaat gcagcattgg aagaaatgtt
     2101 gaagaacctg gattctattg aaattcccca ggaaaaagaa gaacagccgg aagaggaaac
     2161 aacggcagaa gaatcccgga tagaggaagc tcccatagga gaggccgtac cggaactgga
     2221 tctggacagt atacttcaga acgatatgac tgtggacagt gttctgaagc aggagaatac
     2281 agagaatagc aaaggagcag agaagagaat ggacgcaaaa atcgcatcag atgaaacagc
     2341 agttatcacc gcaggcatgg ttatcaccgg agatgtaagt tctgaaggat ctatggatct
     2401 ggtaggtacg atcaacggaa atattgatat tctgggcaaa ctgaatatta ccggatatat
     2461 caatggaaat tccaaggctg ccgagatctt tgcagaggga gccaagatca acggtgagat
     2521 cgtcagcgaa ggttctgtaa aaatcggtgc aagttctgta gtcatcggca acatcactgc
     2581 catcagcgca gctattgcag gtgcggtaaa gggcgatatt gatgtacagg gacctgtagt
     2641 gctggattct tccgccatcg taatgggcaa catcaagtcc aagtccgtac agatcaacaa
     2701 cggtgccgta atcgaaggta tgtgctctca gtgctatgcg gatgtcagcc cgacttcctt
     2761 cttcgatgat tacaagcccg agaaaaagaa agtaaaataa ggagcatatg accatgaaga
     2821 gaatattact gaagcttagt ggagaagcac tggcaggaga gaagaagacc ggttttgacg
     2881 aggagaccgt cagaaaggta gcattacagg taaaggaact ggtagatgac ggtattcagg
     2941 taggtatcgt tatcggtggt ggcaatttct ggagaggacg ttccagtgag aacattgacc
     3001 gtaccaaggc agaccagatc ggaatgctgg ctacgatcat gaactgtatt tatgtctccg
     3061 agattttccg ttccgtaggc atgatgacca atatcctgac accttttgag tgcggatcct
     3121 ttaccaagct gttttccaag gacagagcaa ataaatattt tgccaaaggt atggtagtgt
     3181 tcttcgcagg tggtacggga cacccttatt tctccaccga taccggcgtt gttcttcgtg
     3241 ccattgaggt agaggcagat gtcattctgc tggctaaggc aatcgacggt gtctacaatg
     3301 cagatcccgc caaagatcct acagcgaaga aatatgatga agtatctatt gatgaagtaa
     3361 ttgccaagaa tcttcaggta gtggatatga ccgcttccat tctggccaga gacaacaaga
     3421 tgcccatgtg ggtattcggc ctgaatgaag agaacagcat cgtgaatacc ttgaagggca
     3481 agttcaacgg aaccaaggta accgtataat ttcagaaaat aaaaatggaa aggcgcagag
     3541 cgcagaagag aggaaaacaa tggacgcaag agtacaggta tatagtgaca agatgcagaa
     3601 ggcttatgag tttctggagg cagattttgc tgctatccgt gcgggacgtg cgaaccctca
     3661 tgtattagac aaactgagag ttgactacta tggaactcct acccccattc agcaggtggg
     3721 taatgtaacc gttcccgagg cacgtatcat ccagatcgct ccctgggaga agtctctgat
     3781 caaagagatc gagaaagcaa tcatgacttc cgatatcggt atcaaccctt ccaacgacgg
     3841 cagtgtgatc cgcctggtat tcccggagct taccgaggaa cgcagaaaag atctggtaaa
     3901 agaagtaaag aagaagggtg aggaaggcaa agtagctatc cgtaatatca gaagagacgg
     3961 caacgatgcc ttcaagaagc tggcaaagac agaagtatcc gaagatgaga tcaagggtct
     4021 ggaagatgat ctgcagaagc ttaccgacaa gtatgtaaag gatattgatg ctctgatcga
     4081 cagcaagtcc aaggaaatca tgacggttta agaaggttta aaactatgga agagaatctg
     4141 aaaattccga atcatgtagc aattattctg gacggcaacg gccgttgggc caaagcaaaa
     4201 ggaatgcccc gcaattacgg gcatatgcag ggagccaaga cggtggaagt gatctgtgaa
     4261 gaggcgtacc gtatgggaat ccagtatctg acagtatatg ctttttctac agagaactgg
     4321 aaccgcccgc aggatgaagt ggacgcgctg atgaagctgc tccgcaatta tatgaagacc
     4381 tgtctgaaga cagcggagaa gaaccgtatg tgtgtcagag tcctgggtga caagaccaga
     4441 ctggatgagg atatccggac gcggatcgcg gaactggaag aggccacaaa gaacaacgat
     4501 ggcctgcatt tccagattgc cattaattac ggaggcaggg atgagatcgt ccgtgcagtg
     4561 aaaaaactgt ccgcaaaagt acagagtgga gaagtggatc ctgcagggat cacggcagat
     4621 actctggaag gatacctgga tactgctgga attccggatc cggatctgct gatccgaacc
     4681 tgcaatgaac agaggatttc caatttcctg ttgtggcagc tggcatatac ggagttttat
     4741 tttaccccgg ttccctggcc ggattttaca aaagaagaat tgatcaaggc tgtggaagca
     4801 tataatcata gagacagaag atatggcggc ctgaaggagg aatagcttat gttttggacc
     4861 cgtttgctca gtggcatcgt attgcttgcc attgcactgg ctacgttcag cttcggcaat
     4921 gtatttctgg cagtgccgtt gtggcttatt tctctgattg catacagaga gcttaccaag
     4981 gcattaaaat gtgcaacgga tgagaaaaaa tttaatgcac tggaatggat cggttttgtg
     5041 ggagtgacgg catattatgc aacactgttt ttcaccagag accatacatt actgctgatg
     5101 tgtattgtag cactgttcat ggcggaaatg ttctgttatg tggcagcctt tccgaaatat
     5161 caggcaagtc aggtcatggc agcagtgttc tctttcctgt atgcaccggt gctgttgtcc
     5221 tttgcctatc tgacccgcga gtgcgagaac ggaatttatc tggtatggct gatcctgatc
     5281 agttcatggg gatgcgatac ctgtgcctat gtcgtaggca aactgatcgg aaagaagcat
     5341 attttcccgg aattaagccc gcataagtct ctcgagggat gtatcggcgg tgtcgccgga
     5401 gcagccctga tcggtggttt gtatgcacat ttctttgtgg aagcggcatt ccctgatcag
     5461 atcatcacct ggacaatcgc attcatctgt gcggcgggcg cggtgatgag tatggtcggt
     5521 gacttggcgg catctgccat caagaggaac cataacatca aggattatgg caaactgatc
     5581 cccggacatg gtgggatcat ggaccggttt gacagcatga cggtgacagc accgatgacg
     5641 tattttcttg ccattctgat gattggactg cgttagtccc caaggtaaga tacaaggagc
     5701 ataagagtga aaaataaagt agatttttgg gcgcacttat ataagggttc ata
//
LOCUS       DAWEJW010000134         5752 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4205_length_5752_cov_74.542391, whole genome shotgun sequence.
ACCESSION   DAWEJW010000134 DAWEJW010000000
VERSION     DAWEJW010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4205_length_5752_cov_74.542391"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3650..3749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1385 a         1509 c         1189 g         1569 t
ORIGIN      
        1 tccgtcacat tctcaatcac ctgatctgcc tttaacggtg ccaccttcag gatcatcgcc
       61 ttgatccggt cctggttcag attcagggaa tgatcgtccg catagtacca tgtttcatcc
      121 tctttttcaa aggcataggt ctctccctca tatacatagc ggaagctggt aatatcatcg
      181 gagtttacat ccagcaccgt ctcctgtgta tcctcggtga tggaagtcgc agacgatgca
      241 tttttattat actggcggat gcccaggaaa actgccgcca gcaccaccag tgccagcagc
      301 attccgatca tttgtctttg tttctttttc atatctataa ctcacgcctt tcacaattat
      361 ttcttccgtc tgcggaacca gatcacgaat ccggcgatca tacagccgac gggaaggatc
      421 acggtaacca gcagtcccaa caacagaatc tgtgcggaat ccactaccag attagagacc
      481 tcatagcttt ttatcggaat ggataccgag gtctcatgat ctgccattcc actgaacgta
      541 ttggtgaaca gggtcaggtt cgcaccgctg acgacagagt tggcatcgtc ggtgaacagc
      601 tgctcacaac cgtacacgac cagtgtcgca tccccatcat ccagtgtctt tactgcctca
      661 acacctaatg cgaagggacc atttacatca ttttcaccct gcgaaaaatc ctgtgtattg
      721 ttgacatcct gttttgcaaa agagctgtcg gaggtgctca ggaatacatc ataggtaata
      781 tcgccggtgg ctgtctcgtc ggtacttact tcctccggga cgatgatgcc ctgagaatac
      841 ggcaggaaca gatattttcc gtaagtgccg gaggtatagc tgtcagagct ctgtgtcggc
      901 agtatataat acggacttct gtagtagccg ttggaatcat tctccactac cagtcctttg
      961 gcaatgctca gattcatgta gcttaagata gcatctatat tgggtgtctc ctcatcggta
     1021 tagccggtta ccaggatgac ttttccaccg ttgttcagat agtcgatcac cttatcctta
     1081 tcatcggatg aaaaatcgct ggtggcaccg ttaatgaaca gacaggccgc atcgtcgggg
     1141 atagcatcca gatccatcag attcacggtc tcataatcca cattttcctt gttcagtgcg
     1201 gatgtaaaag tgttgcttaa actcagttca ttatgtcctg ttgtcatata taccttgggc
     1261 atatcatcat ttaacacata atccagcgca ctggtgatct gtccttcgcc atcataaccg
     1321 gtggtcgtgg tgttataact gtacgtcgaa tagtcaaaat cataagaaga ctcatagaca
     1381 tcgttgtaat caatgacctt gctgcgcttg tcgctgacca cgatcaggct gttcgtgctg
     1441 atgttgccgg tggtgtactg ggtatagaac atcgggttaa cggtgggatc cacatattcc
     1501 acggtgatat gatccgacag atcatcgtat ctctgcaaag tctgccccag tgtggtatcc
     1561 tgattgtcct gatttaccaa cacatagatt gtcacatcat cctgcatatt tttcacatag
     1621 tcgacggtct gatctgtcag ggaatacagc ttctggctgg tcatgtcgat tgctgtccag
     1681 ctggaaggca tctcgcccat gataatatta acgactacta ccagcgcaac agctacagcg
     1741 atcataccgg tactgtaagc actgaagctt aagtttttca cggacatgct gtaacggcgt
     1801 ttctgaatgg actgtaccgt caggaacaga accagtgccg taacggatac ataatatacg
     1861 atagaagata catccagtgt gccatttaac aggttggaaa atggtgtata aaggtcaaag
     1921 caacgtagca gctttgtcaa cagattgccg gtgctggaaa taatggagca gatggaatcc
     1981 atcatatatc ccaagaacaa tactaaaaaa gtaagtgctg ctgcaatgac ctggctctcc
     2041 gtcagggagg acaggaacag gccgatggca atggcggtca tcccgaagag gaaatatgcc
     2101 aggatcgaca gataggcctc tcccatggga acactgccgt actgcgccat gatcagcggg
     2161 taaaagcaga tgatcgctac gggaatgaca aatatagtta acagagccag gaattttccc
     2221 ataacgatac cgcctacgga caccggtgct gtcaggatca gctgatccgt cttgctccgc
     2281 ttttcctccg ccagaatacg catagtcagg atcggcacgg tcagcatgaa tagaaaggta
     2341 acgctggata ccacatacgc aaaataagga tatccgttca tcaggttata aacgctgaaa
     2401 tacagtccca ggaaaaacag ggtgactgca ataaataaaa atccgataaa ggaacggaaa
     2461 taggatttca attctctctt ataaatggct agcatatgat tccgcctcct ttgtagttac
     2521 agtttctttt ttctcttctc cgggctgtgc ttcctcttcc gaagcttctg tctgctgtgc
     2581 ggcagcatct gacatttccg gcctttttct ttttgagaat agtttgtgct tctccggctg
     2641 tgtgggtgct acatcctcgg tcagttccag gaagatgtct tccagagact tctccgcatg
     2701 ggtcatctct aaaataggca gctttgcatc tgccagtgca taaaatacct gctctctgat
     2761 atctgcattc tctttggaga tcactttcag ctttgcaata tttttgggag tctcagtatt
     2821 ttcttcgatc cggtcgatct gtgagatctc ctgcaatgca ctcttcaccg cagcggcgct
     2881 tccctttact tccagatcca gttccatgga accactcata agcttctgca gattctccgg
     2941 agaatcactg gctaccagct taccgtgggc tatgatcata atatggtcgc agaccgcact
     3001 gacctccgac agaatatggg agctgaggat cacggtatga ttctccccca gcttgcggat
     3061 cagatcacgg atctcgatga tctgtttcgg atccagacct accgtgggct catccagaat
     3121 gataacctca gggtagccaa ggattgcctg cgccagtccg actctctgcc tgtaaccttt
     3181 tgacagattc ctgatcagac gctgctgcat atcggtgatc tgtgtcattt ccatgacctc
     3241 atcaatctgc tgcttccgct cttttttcgg cacttttttc agttctgcca cgaaatccag
     3301 gtattctctg acagtcatgt caacatacag cggcggaagc tccggcagat aaccgatggc
     3361 acgctttgcc tcctccggat ctttttggac atccttgcca tcgatcgtca cagtaccctc
     3421 cgtggctgcc agataacccg tgatgatgtt catggtcgtg gatttacctg cgccgtttgg
     3481 accaagaaag ccgtaaatct gtcccttttc cacacggaaa gagagatgat ctacggcaat
     3541 atggtcacca tatttttttg taagattgct tacttctatc atatttgcct cccggatatg
     3601 gtgtgatacc cccacctgat atctacgggt acccgtaact gatacccacn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aattctaaaa acattcggaa tccgctgatt
     3781 tgctacgcaa atcgctactt cctcatgttt tacgggtgta aaattcaacc gctttatcat
     3841 gcaagcatga acagcggtta acctttacac ccttgtatca gtatatgtct caaatgtcat
     3901 gaaaaggtga aacatttgtg aaaaatctgt gaagccccaa cccttaaagc ttcgtcacgg
     3961 tcagcgaaat gcttaaattt cctgcctccg ccgccagatg cagcggcagc ttcgccacag
     4021 gaatatcttc aaaaataaac tccggatcac cggatgcggc cgactggaat aaaaaaagta
     4081 ctccatctac atagagcacc acttttttct gattgctgta aattgtcaga gaaagtaatc
     4141 cgttctcctg acgatgcaga gtggaaagta ccggatagag caccggttcg ctgctccatt
     4201 ttgcctgata atagtagtat cgctctgtca gtgtcctgcc atccgccgaa agaagtgcat
     4261 cttctgccgt ttctccggat aatccacgat ataccggaag attttccggt cttatccaca
     4321 ggtccccagt gaggaatcca cgctttctgc acaggctgta aaacgttctc acttcggagc
     4381 agttcacccc ctctgccccg gtcccggttc cttccagccg gatcacattt acccccatcc
     4441 gaaccagccg ttccagatct cttcgtacct gctccaccct cgtcccggca tcactgatct
     4501 gcgggggaag ccggtagact acttcatgtg gcaaaaactc tttttcattg agaaagattc
     4561 cctttttttc tacgacacgc acgtcataaa tggtcagttc ctgccgccaa tacatttcca
     4621 cttccccggt tatatcctca gcatgatctg attcttcctc gttctttgta ccccccatat
     4681 tttctccata ctccacggtt ccgaaccttc cctgtgcctt caccagatag acatagggat
     4741 cccgcatgcc gtcccagaga tgcggccgca acagcaacgc cgtcatggaa tccgcttctt
     4801 ctaatgagta ttccccatga aaaacaaggc gttcgccctc cgcaccttcc cagatctcaa
     4861 tctgtaaagc ggacagcaaa cgccctatct tttccggcag ggcaaattcc agataagcct
     4921 tgccattttc cagttttgtt gtaattccaa gatcgtctct catctgcatt tctctttcct
     4981 gcctttcgca ttctgttact aagagttttt gcagataaca ttttatttat gccggaaatc
     5041 catctttccc gcacactatt attacatggg acaagacctg cggccgggca cttcctctcc
     5101 ggatacataa cctgcatgaa attccatgtt gatgtcccga agctccttct tcccgtcgat
     5161 gtatgcctga tacatttccg ccagtcccga agtacagcca tcacagttgg cttccatata
     5221 tcccagagac tccgccacga tctgctcccg ctcttctttt gtggtatcct taatcagata
     5281 tcctgccatt ttcttttcct ctcttgtatt tcttttacag ccaatgtatc acagcaatct
     5341 gccgggtgca agtttttctc tgcatttaga gtaatcgaaa atctacgacc gaagaattct
     5401 ctcatagatc tgcaaatcct cattcttaaa cacattaaag atcacatttt tgacactgct
     5461 gtcaggagtc tcctccaaat actcctgtac cgtctgtacg gcaatctgcg ccgccttgtc
     5521 gttcggaaag cagaataccc cagtggatat accgcagaac gccacacttt ccagcccgta
     5581 tgccgatgcc agttccagac aggacaagta acaggaccga agcaatgcgc aatgtttctc
     5641 cgtcagcttt ccctgcacaa taggacctac ggtatgcagg acataacggc agggcagatt
     5701 gtaggctttc gtgatcttcg cctgtccggt gggttcctca tgcccctgtt ct
//
LOCUS       DAWEJW010000135         5694 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4250_length_5694_cov_52.099308, whole genome shotgun sequence.
ACCESSION   DAWEJW010000135 DAWEJW010000000
VERSION     DAWEJW010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4250_length_5694_cov_52.099308"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1793 a          897 c         1355 g         1649 t
ORIGIN      
        1 ggatgaattt tggtatgatg tttatttact ttttcctttt attataaagg atttcttttt
       61 gtttcttttg tttagtatag ttggtttttt agttgtatat ctgtttggaa atgttgctta
      121 taaaattgtt ttatattgct attttaccgg tactgtagcc tgttatattc agggaaacta
      181 tatggttaaa aatttacctc ctttagatgg aacagatgtg aattggagtt tatatcaatc
      241 acagtttgta aaatctacta ttgtgtgggt gatcatagca attgtttgtc tgattttatt
      301 tatagtctta aaatatgaca ggataaaaaa ggcggtttct tatataagtg ttttcttatt
      361 attgatttta gtaagtacga ttacagttct ttctattaat aataatattt ttgaaaaaaa
      421 agaatatgca agatttacaa aagtagatca gtttgaaatg tctacagata ctaattttat
      481 tattttctta ttagatgctg tggatgaaga atgcttctgg caagtgtggc aggaacatcc
      541 ggaatatgaa gatgcaatga ctgattttac tttttataat aacgcaatgt cagggtatgc
      601 atacacggag cattctttac cgttaatact ttctggagaa tggtttgaaa ataaagaacc
      661 ttttatagat tatagaaatc gaatttttaa gagttctcct ttttttaatt atttgcgtga
      721 gcagggttat actttaagta attatgatga cgaatataaa tttgaaaaag gcgtcatgga
      781 tggtgctttt aataatatca catatacgaa atcttctctt tgggatcgtt ctttgtttaa
      841 taccagaatt attaaaatgg tgggaatgaa atacgcacct tatgtattaa aaccgtattg
      901 ctggtttaat gtaagtatgc tcaaaaatca ggaaatgggt tccaaagatg aagagttatt
      961 ttcctggaga aatgatgatt tttataagga tgtacaggaa gatgatataa cctatgtaga
     1021 tggaaaaaga tttaaattaa ttcatttaat gggggcacat gttccgttct attttgataa
     1081 ggatgtaaac gtaatagatg atgcagatta ttatacttct attgaatctt ctatgactgt
     1141 tactatggct tatctgaata aactaagaga agcaggagtt tatgataatt ctgtcattat
     1201 catactatcg gatcatggat ataacataga aggtgaagca gtaaaagtag ctcagaaaaa
     1261 cgagaacgaa accggcagac agcatcctat tttatttgta aagggattga atgaaagtca
     1321 tgatttacaa gtatccggag caccgatctc gtatgaggat ctggtagagg cctattataa
     1381 attaatgaat ggcacagcgt ctgatgattg ctttgcatac aaagaaggtg atcagagaga
     1441 gcgcagatat cttctctata aatatctggg agaagatcat atggtggagt atgtacagac
     1501 tggttatgcg ggagacgaat cgacgcttgt tcctacagga agagtgtttg atgcaaaata
     1561 gatgttggca gttcatggaa aggcattagt aacagtatgg aaaattttga aatggaaaca
     1621 atagcagcta ttgccacggc tctatcagat tccggaatcg gaattgtaag aatcagtggt
     1681 gaaaatgcca tatatatagt agatagtatt ttccgttcca catccgggaa aaagattctg
     1741 acaaaggtac agagtcatac gatccattat ggatatattg ttgataaaga tgagaatatc
     1801 attgatgagg ttatgacctc tgtaatgaaa gcaccaaaaa gctatacgat ggaagacacg
     1861 gtggaaatca actgtcatgg tggtgtgttg gtgatgcaga aagtattgga gacagtgctg
     1921 caggccggtg ccagaatggc agaaccgggt gaattcacca agagagcttt tctaaacggc
     1981 agaattgatt tatcccgggc ggaagcggtt attgacgtga tccattccca gaacgaatat
     2041 gcgctttctt cttcggtcag ccagttgaag ggaagacttt cggacaagat tcgcagcctg
     2101 cgggaagata ttttatatca gattgctttt atagaatcgg cattggatga tccagagcat
     2161 atcagcctgg aaggatatcc ggaacaatta gctgaaaaag tcacaggttt cgaaaaagaa
     2221 attcaaaaat tattggcaac cgcagataac ggcagactaa tgaaagaagg catatccaca
     2281 gttattgttg gaaagcccaa tgccggaaaa tcttctctgc tgaatatgtt gttgggagag
     2341 gacagagcca ttgtcacgga gattgccgga accaccagag atgccttgca tgagaccatt
     2401 aatctgcatg gcatcagtct gaatatgatc gatacggcgg gaatccatga gacacaggat
     2461 gtagtagaaa agatcggtgt agaacgtgcc aaaaaatatg ccgtagaagc agacctgatt
     2521 ctgtatgtgg tagatgcttc agaaactctg gatgaggatg atcagaatat catcccctta
     2581 ttggaaggga aaaaggcaat tattctgttg aataaatcgg atctggagaa taaaatcaca
     2641 gaagaaagtc tgaaagaaac tctggaaaag gtgttggagc ataccggaga gattcagatt
     2701 cttcgtactt ctactattga tccttcttcg gaaaattccg gaatggaaga attggaagaa
     2761 acaatccgca atatgttctt cgaaggaaaa ttaaggcata acaatgagtt ggttgtaaca
     2821 aatctcagac ataaagaggc cctgcaggat gctttgaaca gtttgcagct ggtagaacaa
     2881 agtattgagg atggaatgcc ggaagatttt tattccattg atctgaccag tgcctatgct
     2941 tctctgggaa aaatcatcgg agaagaagtg gatgaagatg tggtgaatga gattttctct
     3001 aaattttgta tgggtaagta acaaccagaa ggaaaaatac tatggaacta aagaaacaag
     3061 tagatgtgtg tgtagtaggt gccggtcatg ccggatgtga agctgctctg gcctgcgcca
     3121 gactgggtct gaagacagta atttttacag taagtgtaga cagtattgca ttgatgcctt
     3181 gtaaccctaa tgtaggagga tcttccaagg gacatctggt cagagaattg gatgctctgg
     3241 gtggagagat gggtaaaaat attgataaga cttttattca gtcgaaaatg ctgaatgtgt
     3301 ccaagggacc ggcagtacat tccctgcggg cacaggcgga caaggcggaa tattccagag
     3361 ccatgcgtaa agtgctggaa aatcaggaaa atcttctgat caagcaggcg gaggtctgtg
     3421 aacttctctg ggaagagatt gaagatcaca agaaaaagat taccggttta aagactttta
     3481 ctggtgctat ctatgaatgt aaggctgttg ttctctgcac tggaacttat ctgcgggcca
     3541 gatgcctgtg cggagaatct attacttaca ctgggccgaa cggactacag gcagctaatc
     3601 atctgacgga ttctttgaaa gctatgggaa tcgaaatgcg tcgttttaag acaggaactc
     3661 ccgccagaat ggacaagcgt tctcttgatt tttccaagat ggaagagcag ctcggtgatg
     3721 agaggattgt acccttttct ttctctacgg atccggaatc ggtacagaag gaacaggctt
     3781 cctgctggct gacttatact aatgagaata cccatgagat cattcggaat aatctggatc
     3841 gctctcctat ctatgccggt attattgaag gtaccggtcc cagatattgt ccttccattg
     3901 aggacaaggt cgttaaattt gccgaaaaag agagacatca ggtatttatc gagccagaag
     3961 gtctgcacac gaatgaaatg tacgttggcg gcatgtcttc ttctcttccg gaagatgtac
     4021 agttagctat gtatcgtacg gttccgggat tggaacattg tgaaattgta agaaatgcct
     4081 atgccatcga atatgattgt atcaatgcaa aacagttgaa ttcttctctg gagtttaaga
     4141 atatcaatgg tctgttcagt ggcggacagt tcaacggaag cagtggatat gaagaggctg
     4201 catcccaggg attgatcgcc ggtattaatg cagctatgtc tgttttgcat agagatcctt
     4261 tgattttgga tcgttccgaa agttatatcg gagttttgat tgatgatctg gtgaccaagg
     4321 ataacagaga accttatcgt atgatgactt ccagagcaga gtatcgtctg ttacttcgtc
     4381 aggataatgc ggatctgcgt ctgcgtaaga aaggatacga gattggattg atctctcagc
     4441 aggaatatga tgcattggtg gaaaaagagg agctgatcga tcgtgaaatt cagcgtttga
     4501 aaaatacttc tgttggggcc aataaggaaa ttcagcattt tctggaagca agcggcagtt
     4561 ctactctgaa aacggcggca actctggcag aactgatctg cagaccggag ctgggatatg
     4621 cagcattggc tccgattgat aaggagagac atcctttgcc ttctgctgtt gtggaacagg
     4681 tggaaattga aattaaatat gaaggatata ttataagaca gaagcgtcag gtagaacagt
     4741 atcgcaagat ggagaaaaaa atgatcccag agacattgga ctatgatgaa gttccgtccc
     4801 ttcgtctgga agcgagacag aagctgaagg aattccgtcc tgtttcggta ggacaggctt
     4861 cccgtatctc aggagtatct cctgcagata tctctgtttt actggtatat ctggaacatt
     4921 ataaagcaac aaagtagaat actgcggagg aatacatggc agattataat acagaacaat
     4981 ttgaaaaaga ctgtaaagcg ctgggtgtga ctttaagtga tgaacagatc caacagtttt
     5041 tgaaatatta tgaaatgttg gtggaatgga atgaggtcat gaatcttaca gcaattacgg
     5101 aatacgacga agttatgaaa aaacattttg tggacagcat atccctctgc aaagcctatg
     5161 atgtgacgca gaataaaaca gtcattgatg tgggaaccgg tgctggattt ccgggacttg
     5221 ctttgaaaat tgcatttccg gatctgcagg tgacactgct ggattctctg aataagagaa
     5281 ttaatttttt aaatgaagtg attgctgcat tgggacttac cggagtggag acaattcacg
     5341 gacgtgcgga agattttgca aagcctggaa aatgcagaga aaaatatgat ctctgtgtat
     5401 ccagggcagt agcaaatctt tccacattgt cagaatattg ccttcctttt gtaaaggtcg
     5461 gcggcaaatt tatctcttat aaatctgaaa aaataacaga agagatgaat gccgcacagc
     5521 atgctgtgaa gatacttggt ggaaaaatgg atggacaggt tgaatttaca cttcccgatt
     5581 cggatattta tcggaatctg tttattatta caaaggaaaa gtccacacct gccaaatatc
     5641 ccagaaaagc gggattgcca tccaaagaac ctttgtcata aaacgaagag aata
//
LOCUS       DAWEJW010000136         5596 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4355_length_5596_cov_63.311316, whole genome shotgun sequence.
ACCESSION   DAWEJW010000136 DAWEJW010000000
VERSION     DAWEJW010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4355_length_5596_cov_63.311316"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1496 a         1156 c         1585 g         1359 t
ORIGIN      
        1 tttttcataa cttgtttgtg ccttgagcga agcgaaagga atgacaaaaa ctatggagaa
       61 gtcatggaga aaaaagagag aatgcggata aaagggatca ctatgatact gagctgcctg
      121 ttacttttcg ccggctgcgg ggaaaacggt gggagtgagg aaaatgtctc cacggagacc
      181 gcaggccaga aggtttcgga agaaaacagt ggcatgggac aggctgacgc aggaatggaa
      241 tttgtggaac tgccggtgca gacagagcag aaattgacgg agaatgattt taaggtaatg
      301 aaatccctgg tgggaattca gacgggagag cggcagggca gcggtgtgat ctatgatgaa
      361 gaggaaaaca ggattctgat cgcaacggca gggcacgtac tggcggacga taccggagag
      421 gcccgggtga catttccgga cggaacacag gtggctgcca ccggcgtgga gacagtggac
      481 agctgcgatc tggctttcct gtgggtggac aaggcggaac tgtcagagga agcatggaag
      541 gtctgtctgc cggtgcagac ggatcgggaa gtattcgatg cactgaagga atatgacgat
      601 gtatggatgt acggagggga gagtgggctg ccggtctatg ctttcgtggt ggatccctgg
      661 atctatgtgg aggattttga ccagtatatg cttctgctgc agggactgat cgctcccgga
      721 atgtcaggag gcggagcctt tacggaggac ggtgtgttcg taggaatcct gtgtggcggt
      781 gatgaagagg gaaaagtggc cgtggtgcct tacagcatga tcgagacgga gagaccgtag
      841 cacagttgcg tatgcgagca tccgcacagt gtctgacgaa cattatggct tagttgataa
      901 aaatttaata atcgttttat tgacaatact gtaaatatga tagaaaataa aaaggatggg
      961 tcttagactc agattggagg aaaaaacaat gacgaaaata gatgtagtat ccggattcct
     1021 gggtgccggt aagaccaccc tgatcaagaa gctgttaaaa gaagctctgg acggcagcaa
     1081 gaccgttctg atcgagaatg agtttggtga gatcggtatc gacggcggtt tcttaaaaga
     1141 ggccggtatc gagatcaaag agatgaactc cggctgtatc tgctgctctc tggtaggtga
     1201 tttcggtact tccctgaagg aagttatcag cacttatgct cccgagcgta ttctgatcga
     1261 gccctccggt gtcggcaagc tgtccgatgt cctgaaggct gtggagaacg tggcaggaga
     1321 tctggatgtg gagatcaaca gtgcggtagc agtagtagat gcatccaagt gcaagatgta
     1381 tatgaagaac ttcggcgaat tcttcagcaa ccagatcgag tacgccggaa ccattatctt
     1441 aagcagaacc gacaaagtgg atcaggcaaa gctgaatgcc tgcgtggaga tgatccgtga
     1501 gcataacgac aaggctacca ttatcaccac tcctcttgat cagctggatg gcagggaagt
     1561 actgaagacc atcgaaggcg ctgacaagct ggaagatatg atgaaggaaa tgctggagca
     1621 tatccatgac gatcacgatg aggatgagtg ctgtgaccac gagcatcatc accatcacga
     1681 ccatgacgag gatgaccacg accatgagtg ctgctgtgga catcaccacg atgacgacga
     1741 tgatgaagac gagcatgagc atcaccatca tcatgaccat gatgaagacc atgaccatga
     1801 gcatcatcat gagcatgacg agaactgcac ctgcggctgc cacgatcatg atcatgacca
     1861 ccaccatcat caccatcacg cagacgaagt attcaccagt tggggtatcg agacacccaa
     1921 cacctacagc gcagaggaca tcgagaagat ccttactgct ctggacagcg gagagtacgg
     1981 tgcaatcctg cgtgccaagg gtatggtacc cgctcccgac ggaacctggg tatattatga
     2041 ttatgttccc gaagagcatg acatcagaag cggtaagccg gaagttaccg gtaagatctg
     2101 tgtcatcggt tccaaactgg acgaggacaa gctggcagaa ctgttccgta aataaattgc
     2161 cggaacggat tttacaggca gcaggataag aaagagaaag gtatgaatta tgacgaaacc
     2221 agtctatatc ataaatggtt ttctggagag cggtaagaca gaatttatca catttacact
     2281 gggacagccg tatttccaga tcaaaggaaa gacactgctc atcctctgtg aagagggcga
     2341 gaatgaatac gatcccgctt tattaaaaaa gagtcgtacg gatgtcgtat tgatcgaaga
     2401 ggaagaggag tttactccga atcatctgat cgagctggag aagcagtaca agcccgcccg
     2461 tatcatcatc gagtataacg gtatgtggaa ttgtaagaac atgacgcttc cctggtactg
     2521 gaaagtagag cagcagatca cgaccattga cggctccacc ttccctatgt actacacaaa
     2581 tatgaaatct ctgctggcgg agatgatccg taagtccgag atgatcattt tcaaccgttg
     2641 cgacggtatc aaggatctga atgtatacaa gcgcaacatc aaagcggtga atccttccgc
     2701 agatgtgatc ttcgaggatt ccaacggtga gatcgatgag atcttcgagg aggatctgcc
     2761 ctacgatctg aatcaggatc ctatcgtact ggacaaccag ggctacggca tctggtatct
     2821 ggattccatg gatcatctgg agcgctatga aggcaaaaat atccagtttc tggccatggt
     2881 actgaagccc gaggaatatc ccgatggcta tttcgtaccg ggacgcatgg caatgacctg
     2941 ctgtgcggaa gatatggcat ttctgggcta tgcctgtgag tttgcaggca cgaaagacct
     3001 gaagcagaag gaatgggtca aagtgacagc gaaggttgcc agagaatact ttgcagacta
     3061 taagggagag ggccctgtac tccatgccat cagtgtggag aaggcaaagg ctcccaaaga
     3121 tcccgtgatc agtttcaact agaaaggatc ggctctatga aaaagaatcc cataaagagc
     3181 ggcctgcggg agacaatggc aggaaaagtg acatttcttt tcctgctgtt tttgtatacc
     3241 ggggttatgt tatatctgtt ttggatggag tgctatcagg tgccgggctt tcagtcggat
     3301 atgccggact atgtgaataa ggtggcaggt attgcgggaa attatgagtt tccctatccg
     3361 atcctgttct ggacagccag actttccgcc tggctgatcg gagcgaaagc ggccatggcc
     3421 atcaccaccg cgctgttcaa tctggcagcg gtagtgatca caaaatatta tatgaaccgg
     3481 gagatccgga aagtaagtca ttatgatgac ttaactcagg gaagacaggc tatgacggac
     3541 attcttgtga ccctgcttgt cttttcgcta tttttgcttt ctaacctgta ttctcccaag
     3601 aatacagctt ttttcggctt tgattatgcg tatcgctgta tggggattta tacgccgaat
     3661 cctttctgga atgccactta tctggccacc agaccttttg cgattatctg cttttttgag
     3721 acggtgaaag tgctgtcgga atatcagaag gattttcagt ggaagaactg cgttctcttc
     3781 gcggtcagcc ttctgctgac caccatgacc aaaccaagct atacgatggt agtggtgcct
     3841 ctgatcggtc tgatcctcct gatccagctg atcgtaagca gaggaaaaag ttttcgcaat
     3901 gccttctgtc tgtgtgtgac catgatcccc acgggaattg ccctgctgta ccagttttcc
     3961 ggaattttca ccgggacgaa tgccatggga gaggagacag gcatcgccat cggctttgcc
     4021 aaagtatgga gcaattattc caaaagcatt cctttaagta ttatcatggg aatggcattg
     4081 cccatcggcg tacttttcct gaatctggta tttgatttta aaaatataaa gtccaacaga
     4141 tattactggt ttgcatggct gaattatctt atgggtacag taatgttcct gatcttctat
     4201 gaaaaaggat tccgcatgat gcatgccaac ttttcctggg gctatatgca cggtatgttc
     4261 tttgtcttcc tgatgacgtt gatcgtcatg gtgcggaata tcagggagtg gtggaagagc
     4321 tggaaggtga tcttcgtcgt gggcgagatc gcggtattct gctaccatct ggtgtgtggt
     4381 gtgaatttcc tgatgtatgc cgtcctgggc aacgatctgg cgggatttta gtaaaaatga
     4441 tttgaaaagg cagacaagat accatcttgc tgcgagttac atagaataat aaaagaaagg
     4501 agagtgtttc atgaatttaa gcattgtagc cggtcttctg attccgtttc tgggaacgac
     4561 tgcaggggca gcctgcgtgt ttttcctgaa aaaacagatt ggcggcaata ttcagagaat
     4621 atttaccgga ttcgctgccg gtgtcatggt ggcagcttcg gtgtggtctt tgctgatacc
     4681 ggccatggat atgtgcgaag agatggggaa gcttgcattt cttcccgcag tgatcggatt
     4741 cctgatcggt attgtttttt tgttgttcat cgatagcatg gtaccccatc tgcatgtggg
     4801 aagcgatgca ccggaaggcc ataagagcag tctgaaccgt acggctatgc tgatgttagc
     4861 tgtgaccatt cataatttcc ccgagggtgc ggcgtgtggt gctatttttg ccggcgtact
     4921 gagtggtgac ggaacggtta cgatggcggg agccctgacg ctggccatcg gtatcgccat
     4981 tcagaatttc ccggagggag ccattatttc cctgcctctt cggagtgaag gaaataagag
     5041 aggaaagtcc tttgccttag gcgtattgtc gggagcagtg gaaccggtgg gagcaatcct
     5101 ggccattgca ctggcaagca ttgtgacacc tattttaccc tatatgctgg cttttgcagc
     5161 cggagccatg atctatgtgg tcgtggagga actgattccg gaagccagtg agggagagca
     5221 ttcgaatctg ggaaccattg cctttgcgat tggttttgca ttgatgatga tgttggatgt
     5281 ggcactggga taatgtgtac agggtaatat ttgcaaaagc aaagtgagaa cagtaggaag
     5341 gatgtacggg aatgtccgtg gaaaatgaaa aagaaataca acagctgaaa aaccggatcc
     5401 gtgaactggc agacaaatcc tacaaccaga accagtacac atttaccgga ttcttgggac
     5461 tggcggagca ggatgccctg tggcaggtgg aacgggaagt gaagttcgca gggatcacct
     5521 tatacggcgg cagggaagag gcagagcgga agctccttcg cttcggcagt gaggcggagc
     5581 tgggctatga acagcc
//
LOCUS       DAWEJW010000137         5590 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4360_length_5590_cov_68.848419, whole genome shotgun sequence.
ACCESSION   DAWEJW010000137 DAWEJW010000000
VERSION     DAWEJW010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4360_length_5590_cov_68.848419"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1353 a         1493 c         1298 g         1446 t
ORIGIN      
        1 catcgagaat caggacattg ggtgccccca tcagtacctt gcataattgc agtctgcgcc
       61 gttctccacc agacagtttt cccagctgtc cgtactggtc ttccccggaa aacaggaacc
      121 gctccagcat tctcgctgcc gtgatctgtc catcctccgt ggtgatatat tctgccacat
      181 cccggatata atcaatgact ctctgctccg gatgcatcat ggtgtcgtcg atctcctggg
      241 cataatatcc cagcttcacg gtctgcccaa tctcaatatg tccgctgtcc ggagccactt
      301 cacccaggat cattttcatc agcgtggtct taccactgcc gttagggccg ataaatccca
      361 cccggtcgtc cttgccgaac agataggtaa aatcacggat caacacttta tcgccatagc
      421 ttttggatac attctcgatc tctactgtgg tacggcccag tctggatgct gcggaagtca
      481 gttccacttt gccgtccgtc acgggtccct tcacattttt caattcctca aaacgctgca
      541 atctggcctt ctgcttcgta gagcgggctc tggcgccccg ctgtacccag gcaagctcat
      601 tgcgtaggat attcaggcgc tttttctcac tggcctgtgc catctcctcc cgttgcattt
      661 tcagttccag atagccggaa taattggcat cataggaata aatcttccct ttgtccagtt
      721 ccaggatccg gtcagtgaca ctgtccagaa aataacgatc atgcgtaacc atgatcatag
      781 cgcctttgcg gcggcgcagc tgatcctcca gccagtcaga catggaattg tccagatggt
      841 tggtaggctc gtccaataac aggatctccg ccggggacaa cagtaccgat accagtgcca
      901 gacgcttgcg ctgtccgccg gacagctgcc cgcagggctg atcaaattcc gtaaccccca
      961 gtttcgtcag catgcttttg gcatccgcct cgatggttcc ggcgttttcc tccatgcggt
     1021 tgccccgaag caccgcttcc agtacggtct cttccggtgc gaactccgga ttctggggca
     1081 ggtaacgcac cagacagtga tttgccttga tcacggtacc gtcatcggcc tcctccagcc
     1141 cggcaatcat ttttagcaaa gtggattttc cggttccgtt aataccaatg attccggcct
     1201 tttccccttc ctgcaggaaa aaggaggctc cgtcaaaaat cttgcgtact ccataacttt
     1261 tggtaatatt gtcaacagtc agtatattca tctgtataac ctctttttag aatttacgca
     1321 tagcttatac gaactttttt ctcaaagaat cgtatcattt ttcatgtgct atacttccaa
     1381 atgtagtata ctataaataa tctattttag gaaagaaggt tttcgcatga acttaaaaga
     1441 tgctgctgcg aatctgaatc aggcgcaccc ggaagacatc ctgcatcccc tctatactct
     1501 ctggggggaa tcgctggtgc gatccggttc gtatgacgat atcctgccgg aatatccccg
     1561 tccgcaaatg aaacgaacag attaccatat gctgaacggt ctctgggaat atgcttttgt
     1621 gcccgcagat gggagtagtt cttcggaaga ttctgcgtat tttaccgctc agggttccat
     1681 ccgggtgcct ttttcgccgg aagccctgct ttccggagtt cacagacggc tggaacccac
     1741 ggagtacctg tggtaccacc gggaactttc tttttccacg gaagagcttt cccaaaaaga
     1801 ggaaaatatg cgttgcattc tgcattttga cgcggtagat caggaagcaa ccgttttcct
     1861 cggcacaaag aagcttggca ctcacagtgg cggctacctt cctttttcct tagacatcac
     1921 agaatatgta aacgcagatc cggtatccct ctatgtaaaa gtccgggatc tgaccgacac
     1981 cggttacgcc accaggggaa agcagaccct gaaccgcggc ggcatgttct acaccgccca
     2041 gagcggcatc tggcagagtg tctggtacga atgggttccg gaaaactatg tcatcaatta
     2101 taagatcact cctcagtatg acaaggcttc cgtaaccttc gttctgtgtt ctctgaagcc
     2161 gttcaaccat ctggagttcc gggtgaacat tccctcctgc gacggaaccc tcacgcagga
     2221 aaataccggt tcactcacgc agatgcgtgt acgtaaaatt tctgagcaac aggattcctt
     2281 tgggctcacg cataccacgg ttgtgctttc ttttcccgcc agcgtcacct attctgcaac
     2341 ggatcctgct gatgcaccgg agccggtaaa tttcaagccc tggagcccgg aacacccctg
     2401 gttgtatcct tttaccctga acgccgatga ggataccgtg gatggttatt ttgccatgcg
     2461 atgtttctca gtcgaaaagg attccaaggg aattttgcgg ttttgtctga atcacaagcc
     2521 ctattttctc cacgggatcc tggatcaggg ctactggtcc gacgggctga tgactgctcc
     2581 ctgcgatgag gcctttgtct atgatatcag cctcgcaaaa gggctgggct tcaatatgct
     2641 ccggaaacat atcaagctcg agtctctgcg ctggtattac cactgcgatc gtctgggaat
     2701 gatcgtatgg caggatatgg tcagcggcgg ctctacctat catatgccct gggtatgcta
     2761 tatgcccaca ctgttccccc acatgtccgc ccacacgaag gataatcatt atgagctgtt
     2821 ctcccgtggt tcggaagaag gccggaaaag ctgggaacag gaatgtcttg acaccataga
     2881 tcatctgtat aatgtccctt ccatcgccgt atgggtgccc ttcaacgaag gctggggaca
     2941 gttcgatgct gcccgtattg cgaaaatggt ggcaaaaaaa gaccccaccc gtccggtaga
     3001 ccatgccagc ggctggttcg accagaaagg cggagatttc cgcagtattc ataactactt
     3061 ccgtccgtta caggtaaaat tggatggaaa aagggctttc gttatctcgg aatatggtgg
     3121 ctacgcctgt cacatcgacg ggcattccag tgtggaccgg gtctacggat atcagaaata
     3181 tgacaccccg gaggagatgg ctgctgccct aaagcagtta ttttccagac aattatttcc
     3241 tctgatctca aagggcttga gcggtgcggt atatacgcag ctctccgatg tggaagaaga
     3301 ggtgaacggt ctggtgactt acgaccggcg tatcgtcaaa ctgcctgtgg gacatccgtt
     3361 tgcggcacca tcctcttttg cagcacctta ctcacatgga aataagccgg aataaggaat
     3421 gcatccgcca tgatccaggc aatgggactg cccaggcata ccccggtgaa gccaatgatt
     3481 ggcaccagtc caaagcctgc cagggatctg gcgatcattt cgaagacacc tgctaaaatg
     3541 gcgaatttcg ggaaacccat accctggatc aggaaacgga caatgttcac cagtgcaagc
     3601 ggaatataga atgccgtatt ccataacagg aaagcacaca cgttggcaat gatctctgcc
     3661 tcgccgccat ccacgaacag ctgtgccagc ggaccgccga aaaatactgc aatcaaaaat
     3721 gcgatcacag agtatcctac ccccaacagt acacaggatt taagtccctt gccaagacgg
     3781 ttcagtttgc cggctcccac gttctgtcca ccgtaggttg ccatagtgga acccatggca
     3841 tcaaaagggc aggctaagaa gttaccgatc ttgccgcctg cagtcatggc cgcaactgcc
     3901 gcagaaccta aggtattggc tgcagtctgt aagattacgc tgccaatggc ggtaatggaa
     3961 tactgtagcc ccataggaac tcccatgcca cacagcacca tcatcatatg cccatccact
     4021 ttccattctt ccctgtggat tcgcaggatc tcgaattttt tcaccatata gaccagacat
     4081 gccacacctg agactccctg ggagatcacc gttgcccagg ctgcccccgc cacgcccatg
     4141 tgcagggata tgataaaata cagatccagc acaatattaa taatggaaga cagcactaag
     4201 aataccacag gagtcttgct gtcgccggtt gcacggatca cgcctgccac gatgttgtac
     4261 aggaataccg tgggaatacc gataaatata atgaaaatat agttatacgc cccatcaatg
     4321 atgttggcgg gcgtcttcat ccataccaga atgtcccggc acaggatcgc cgtcaccacg
     4381 gtcatgatga cggaaaaggc aagtcccagc caggcacagt tggccacata tttgcgcatg
     4441 cccacataat ctccggcacc gaatttatgg gacacgggga tggcaaatcc attgcagaca
     4501 cccatgcaga aaccgatgac caggaagttg atggagccgg tagagcctac tgctgccaga
     4561 tcatccacac caagaaaccg tcctacgatc atggtatcta ccagattata gaactgctgg
     4621 aataaaaatc cgaataataa cggcacagca aagctgatga taagtttcat ggggctgcct
     4681 tttgtcaggt cttttgtcat ggtaataacc tctccatttt tcaaatcaac cgcaagcact
     4741 gaatttatca gtgtttcctg ttatttctat cgttgagagt atatacgcgg cgctccaaaa
     4801 aagcaatatc tttttttgca cattttatgt ttttttgcta tactcttttt gtcatttttt
     4861 tacaagcatt gttattatga gaggggaatt gtcatgaacg aatttttgaa atatctgaaa
     4921 atgatacctg taattatcta tccttatatt tatgtattga ttcttgctgt cttcttcctg
     4981 tttgtaggcg tactgccaga ggataccacg gatatctccc tgcttgtcct gcttatcatc
     5041 gcagtcatct ataacctgta ttcctttgtc attgtaatcg tcaatgcggt acagaccgcc
     5101 aagggaaaat tgactgcagg gcaggctgcc cggatgaacc tgatcatcaa aggaattcag
     5161 attcctgctt atatcatgca ctttatcctg ggtttcatcg gacttgccat gagtgtctgg
     5221 ggcatcggct ttctcctgtg ggcagtactc attgatcttt tgacgatcct gctcaccggc
     5281 atcagttcca tcggctgcag tattcgcatg cgcaaagaaa ggcttgtctc cctgccgggt
     5341 gccatcttca tggggatcgg atgttttatc tactgtgcgg atgtggtgat cgccattgtg
     5401 tatgttgtga aagcccggaa gaaatctcct gctaaatagc aatttagcga aatatccccg
     5461 tagcccttgg cagggtataa gaaacgcggc acgctcgcgc tctgtcgcag agcgtagcgg
     5521 tacataagcg gtcatagtac gaccgctaag taccgacgtc tgcttacggc actgcttatt
     5581 cttataccca
//
LOCUS       DAWEJW010000138         5487 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4427_length_5487_cov_59.967599, whole genome shotgun sequence.
ACCESSION   DAWEJW010000138 DAWEJW010000000
VERSION     DAWEJW010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4427_length_5487_cov_59.967599"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2043..2142
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1441 a         1545 c          989 g         1412 t
ORIGIN      
        1 atcacttatg ttgttgtata gcttattcca tcaaatcccc cctttccatg gcataaaaaa
       61 agcaaccgga acatctctgt ttcgattgcc atgataatct tactacaacc acaataaaaa
      121 taaaagtgac tatatattag ttttttacta gttttatacg taaatttgtt agttcctcgt
      181 tagtacgctg ttagtctatc gttagcctat ggtcagttca aagatcagtt tttacactca
      241 aagcggaacg tacattcttc tctaaaacta tttttcaaca aggcaattac taataagttt
      301 ttccagcata tcgttgctgg catttgccga tctgacactg gcggctatca tctcttcttt
      361 tgaaacatcg gtaaaatcaa tttcaaatct accattgttc aagcataact gctcaatgta
      421 tattctctga gtccttccat taccttctct aaacggatgg atcatattaa tctcacccag
      481 gtaataggct atagtctctc gctcatttct tccttatcgg ttatgtcagc taagaaattt
      541 tcctttttca gcttacgata caaatcatca aactggcttt cgataaactg caccaaacag
      601 aaaacggttc ctttggaaat atcaaccgtt ctcgtcttgc ccgcccaaga gtatacatca
      661 ctgaataaat gcctatgtat agaacaaaga tgcttaaaag agaaatctcc agtaacgcct
      721 tgacctacaa gttctgcctg acgcaccgct gaataatctc tttcagcctc atgcaatgta
      781 tctaaatctc ttatatccag tttattcttt aatacatttg ttcccggata acagtacttg
      841 gcatcctgtt catcctcata gaaataatca tactcttgat ccttcaacct gcttcttgga
      901 tactctgtat ttcttattta attctgaaat tgcatctgta gcagaaactt tcttggttaa
      961 gacatttctt actctttgac gacattctgc atcaaatggc atgctttcca ttttgaaact
     1021 ggattcaata ctgcttactc ttctatcaac tatcatacgc gtatcctttc ccctttccct
     1081 ttcacagaaa ggtatttgtt cttctatagt ataccacaaa acgctacatc tatactacaa
     1141 atttccgtat tatgaagagc gaacatagtt tgcaagctta tcaccaaaca cgttcgagcc
     1201 cttgtaaatc aagcatttcc gccactttca gccacttact tttcatccac cacctgaaag
     1261 atgtagaact tgaggtaata actctcgtcc gctgcccaca ggattggatg atccgggccc
     1321 tgtgtcttga attctacctg gcggagacgc ttatgcgcac catgagaagc ctcccggatt
     1381 gttttggcga aaagctcagg atccataaaa tgagagcagg aacacgttac caggaatccg
     1441 ccgtccttca ccagctttaa gcccctaaga ttgatctcac gatatccttt tacagcattt
     1501 ttgatggaat tgcgggactt ggtaaaagcg ggcggatcca ggatcaccac atcatatttc
     1561 tcgccctgcg cttccagctc cggcaataac tcgaagacat ctgcacatcg gaagcttacc
     1621 gtattttcca gtccgttcag cttcgcattt tctgttgcct gatccacagc cagttgggaa
     1681 gcatccacac ccagaacact cttcgctccg gcgattcccg cgttcaaagc aaaggagccg
     1741 gtatgggtga agcagtccag caccttttta tccttacaga aacgatgaat ggcggcacgg
     1801 ttattcttct gatccaggaa aaatccggtc ttctgtccgt cctctacatc caccatgtaa
     1861 tgcacaccgt tctccacgat ctctactttc gtgtcaaacg gttcgccgat gaagccttta
     1921 taacgctcca taccctcctg taagcgtacc ttggcatcgc tccgctcgta gacaccgcgg
     1981 atggtaatgc catcctccgc cagtactttc ttcaaagcat caatgatcac aagcttccac
     2041 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccacgagg tccgggggac
     2161 ctcggcctgg accgaccgaa gcggagacca taaattcaac tttcgctggt tcaatcacgg
     2221 gctgaacgcc gcaaattctc tccaatcccc ataatcacaa acaccgtcga aatattcatc
     2281 acctgctgga agctgaacat attgttcacc gtataggcca gcacacagaa cccgcaggct
     2341 cccaccagca tcgacttttt cccggcatgc aggaaattac ggattactga gatcatcatt
     2401 cccgcataac tgaccagccc cagcactcct atatccacca ggatcgtcag ccattcatta
     2461 tgtgcattag tgaggcggtt tccatagaag aaatcataca ccatattttg cagctccggg
     2521 ctggcctcat ttgacagaaa cgcatacatg caatccggcc ctacgccgaa aatcttatgt
     2581 aacagatctg cttcccagaa gcagctggct cccgccgacc aggtggctcc cctgttgctg
     2641 ccccaggccg cgctgaacat cagccagttg ctgcccccgt cacctgccgt ctccgtcaga
     2701 aaggacgtca gttttccgcc caacagcgta ttcgccagaa taaacgctaa taatgccacg
     2761 ctcacaaccg gcagtgctac gcacaatatc cgatagatgc cccaaaaaag tttcccacgg
     2821 taatgatttc tgttattgct ggtatgtacc agccacagca ccactactga cacgatcgtc
     2881 ataatcacac tcattgcgga aaacgtcagg atatccgtca ctttttccat cggcagttcc
     2941 tgcgaaagca cattgcagct ccgcagaata tatgtgatca gacatgccac cgataacaat
     3001 gtcatttcct gccagaagca ttccattttt ctgccatctt tcacagacat gccaaacaat
     3061 acaaacagca ttacggctaa agttacaaca ccactgctgc tgccctgggt taccagagaa
     3121 gcaaaaccaa tcgtcacata ccccatcagc agcgctctct tccaagtgat ctccgatccc
     3181 atccaccata acagataaac tccaccgaac agcaccgtca ccagataacc gcaataccag
     3241 ttaatatttc ccacggtaga aataaaggag gaattgatga tctccttatc tacaaggcac
     3301 agcccgaatt tattcatata tccgaggaaa aaaactatgg tcgacaccgg caatgtcagg
     3361 atcacgatcc agtcgtttct ctgccacatc cgtgagatca taaaatatac aatgaccgct
     3421 cccagctggg tccacatccc catccgccag gataccgtcc cccacagtgc ctcctcccga
     3481 taccgggtaa acacatagga cattaccacg gcaatcccgt atagagcagc aaatttatcc
     3541 gtggtgttca aagcctgcca gattcttttc ggaatagtcc gaaattccgg aagctcctgt
     3601 ttctgcttta ccacaatgaa gcgaaggatc agccacaaca caaataccgg aagcagacat
     3661 ttccccacat aagtcatagt cttaataaaa aaattctcct tgtctgttcc gatctgggca
     3721 tagcccctgt tgtacaacgg cagtacagcc aatatcacaa acagatacac gctgatcacg
     3781 atatcacaca ggaaccggct gaaattgcat agcgtttctg ccaccgattc cacagaattt
     3841 ttcccttttg ttttttttgc catagatccg tctctcctaa gcttcctttg tctaatactt
     3901 ttatgatagc tttcgacact tctttcgtca atcttttctc attcctgttg ttcctgctcc
     3961 ctctggcggt catacatgcg ggccaggctg tgataagtct catagatgag cacaaatccc
     4021 aatggcccct tgataatccc ccagacgcca aacagccgga ttccggcata caacgacacc
     4081 agcactgcta tgggggatac ccctattttt ctgccgatga gacgaggctc catgacctcc
     4141 cgcagaaaaa tgcagaccac atacaataca ccacagacca gtgcccggcc ccagttgcca
     4201 tccaggatct gaaacatgcc cagcggaacc agtaccactc ccgttccgat aaaaggcagc
     4261 gcatctaaca tccccgccag gatcccccac aaaataccat gttttattcc ggcgattccc
     4321 aatgttgccg cggctgtcat ggcaatcgtg gtcataatga tcccctgggc ctttacaaaa
     4381 gtggcaatat aacggacgat cccgcagatc acctccagaa gtacatgaca ggcctcctga
     4441 tccagcagac ggttcataat accgtcatag tctttcgcca gcagcaccga agcgatcaaa
     4501 aacgtcacca gaaatcctcc cagctccgcg aaccactgca catactgcat agactgggaa
     4561 agcattcccg gcagaatatt ttcttccagt gaatccagcc cctcctgtaa tctgccactg
     4621 accgtatttt ccagatacgc agtgtccaca ccaataatct ctcccacgct gcgacacagc
     4681 ccccggacgg cttccagcca ctcctgctcc cgcagttcca gattgccgat ccataccggc
     4741 aggctgccta gcatccagga caccagaatc cagagtaacc ccaccagcaa cactgttgcc
     4801 acgatgagaa gcactatggc actgaggggt ctgggaaaat gcagacgctg attcatccgc
     4861 ttcatcattg gcccaaaaat tgtgacaaac agcatggcca acagtaccgg tgccgacaga
     4921 ggtgtcagat attgcagaaa aaaatagact gcccccgtta ccagaagcag tactatcatc
     4981 ttttttccta ttattttttt gccaaattct ccccaatcca tgccgcacac ttcctttttg
     5041 ggattagtgt gtgccggaca ggctttttta tccttcgaaa atatcctccg aaggaatttt
     5101 tgtaaatttt tcaaatgcag gattctgcgg tttcacccgg aatgacatct ttttgccgct
     5161 gccgggatgg gtaaaatcca gagaatatgc gcaaagtgcc acgccgccat atgccggact
     5221 ccccgccgtt ccggcgactg tagtgttccc gtatttggta tctcccaaca acggccatcc
     5281 cgcatgggca aactgcacgc ggatctggtg gaaccgtccg gtctcaatgc agacatctgc
     5341 cagtgccagc ggcataggct gattaatttt ctccaaaatg ctataatgca gttttgcgat
     5401 ctttgcgtct ggaaaattct tctctctgcc ggtgactgcc actgccacgc tgccctcttt
     5461 ccgtagataa tccacaagct ccccttc
//
LOCUS       DAWEJW010000139         5460 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4449_length_5460_cov_49.406290, whole genome shotgun sequence.
ACCESSION   DAWEJW010000139 DAWEJW010000000
VERSION     DAWEJW010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4449_length_5460_cov_49.406290"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1587 a         1154 c         1258 g         1461 t
ORIGIN      
        1 acgggcgtgt gtctgccgga tgtatccgtc tattcggaac tctgccagat cctcggtatc
       61 agcatcaatg aattcctggc aggagaagat attgtccggg aaaatattgc caaaaaatcg
      121 gaagaaaata ttatcgggct ggcaacggac agtaagcaga agcaaaaacg attgaaatac
      181 attataggta ttctgcttgt cacggcggcg attgttgttt tgacggtcgg aatcatgata
      241 ttccggaaaa atatgccaca gaactttatc gttccggtgg acaaggacag tattgaaatg
      301 aaaacggcgg aaatgctgtc aggcgtagac ggtgccttta tgtataaata cacggcaacg
      361 aatgaattta aaactctata tattcatatt tggcaatacc gggaaggaaa gctggtagac
      421 agacaaaggg cagggctttc gtatgaagga atggattcac cgcagaatgg aacgattatt
      481 attgtaccgc agtttaagga tcgtaccgtg aaaattatta ttgcagatga tgaatcgcag
      541 ggagatatgc taatcccaat cctggaggat gttccggaag gagagcacta tggatatgga
      601 agagctgcaa cggaaattca ggagaaaaca gatattgtct atgatgaaca gcagccgctt
      661 cttgcgctga tctatgataa tgacgaagcg agaacttttg attttagtta tattgtgaac
      721 gaccagctgg atgcccttga aatggatgat tatgtgtact atttttcttt tgagttccga
      781 acggaataga taaataaacc ccattccgtg aaaacagaat ggggttcgtt tttagtttgc
      841 tgcttttgcc tttttctctc tctttaaaaa caggtacaca cagataccgg caaaagcaat
      901 cgttgcgacg atcagtatcg gcagtaccgg tatggaacgg atatctgtac tgatgagctt
      961 cagtccctga tacagtgtga agacgccgaa tacagagaag atagcaaatg cgagagtatt
     1021 gagcagtcct tccggagtct tgctctttaa cagatagcct aaaagcattc ccaggatgtc
     1081 tgctaagaac agaccaagag agcagccaat ccatacgatc agtgcggaac tcataccttc
     1141 attggcacca aaggtgatgg cggtaagctg tgtcttgtct ccgagttcag ctacgaaaaa
     1201 ggtacagaaa actgcaaacg gtgcgaaccg gatcctggat ctgccgttgc cctcctcttc
     1261 ctcgtcatca tcgcccgcaa gtgttgatgc ggcaaaataa agaaatgcaa gtgcagcaat
     1321 gatcttgata agccagccgg gaatgaattc gctgacgagc ccgccggcga ggacagccag
     1381 accgttcagt accaggattg cggcagcggt tccaaatata atatctctta atttgtattt
     1441 tgaagtaagg gcgatcagca taagctgtgt cttgtcgccc atctcagcga taaattccgt
     1501 gagaaatacc ttaaaaaaca atatcatttg tacggccctc atttatctga ttatgatata
     1561 aaccgcatat gctgcgtaaa gagccaccat acagaagcct tcgactcttc cgattttctt
     1621 tttgcttcct gcgaagatcc atacacacac agtaaatgcg atcagtacac acagatcaat
     1681 gatgttttcc gtaatgatgc tgatgggact gatggcggaa gcgataccca gaaccatcag
     1741 gatgttaaat acgttggaac cgatggcgtt acccagtgcc atatcgactt cattttttct
     1801 ggctgcaacg atggaagtca caagctccgg aagggatgta ccgatggaaa cgatggtaag
     1861 tccgatcagt gtctgcgtca ttcccaggtc gcttgcgatt ctggatgcag catcgacagt
     1921 gagatcccca ccgaaggcga tcgcagcagc accgccgaca atgaaaataa tgctgagcgg
     1981 tacggagatc agcttgatct cttcatcgga accgccctca atttctactt ttttcccctc
     2041 tttatttgct tttaaggcaa tgctgatcat gtagaagata tatccggcaa acagacctat
     2101 gaatatcaca ccatcgaaat gtcccagtgt cattttggtc tgatcaccaa gcccgatgct
     2161 tcccaaaagc agtaaaagac cggcacagat cagggaaagg gggatatctc tccggatagt
     2221 ttcccgcgca acctccacgg tagcgatcgt tgcacatacg ccgatcacga ccatcagatt
     2281 aaagatattg gaaccgacga cattactgac cgcaagctca ttgttgcctg tgatagaagc
     2341 cgatacactg accgctgtct cggggagaga ggttcccatt gctacgatgg taagcccgat
     2401 gatgatggac ggaacatgca gtctcttcgc tacactggag cttccttcta cgaaaaagtc
     2461 cgcaccttta atcagcagta caaaaccgat tacaaggaat acaagaacaa gggcaaacgg
     2521 ggcattgtta ataagtttct ccatgttttt ctcctttgcg tgatccatta tagtgttgtc
     2581 agccgaaagc tgtgcgatga taagtatcga cagagataaa aaagactcat gtatatcaat
     2641 tatctgccag tgtgtattat acacagaccg ttaatagata tacatgagtc tcattattta
     2701 agatttgcca gattatactc taaaattata atcagaatgt tgacaaatca caccttcgtg
     2761 ctaactactc cctcatggaa tattcatttc ttgataaata atatcaaaaa gaattgccgg
     2821 tgtcaacagg tgaatgaaga attcgtaggg agtgttttgc attattggtt tgcactgtct
     2881 gcatcaatta ccgattgaat ctgctgcatc agagagtcat agttatcctg gttatcaatt
     2941 cccccaagga tcggatcccc tacaatattg ccatttctat cgacaagtat ggttgtagga
     3001 aaagccataa tatcagaggc atatttaccc gcatcggaag cagagtcaat ggaaatattg
     3061 cggtactttg caccctggct ttcaagaaca gcggcagctt ccttcatggc agcttcattg
     3121 ccgtcgaagg tttccgtatt gatgccgatg acttcgccgc ccattgcttt gagcgtattg
     3181 tttaattcgt tcagcataga caactcagca acacagggct tacagccggt aaaccagaaa
     3241 ttgacgacgg ttactgcatt ggcggaaaac aggctttcat cgacagaatt accatcaaaa
     3301 tcctgaccgg agaaattccg gaagggagag tcagcttcgg aattgttttc cggagcagta
     3361 tgatcggctg cggtagttcc ctgttccagt tctgttatct gttcttcgat tttccgtatg
     3421 gtttccatat cgtccgtaag tgttttcaat tcatcctctg taaaggagtc cttgtttgct
     3481 tcgacggtat cagccagata atcagcataa tttttgctgg gatcagcatc gcttttgctc
     3541 atcatgccga ataccttgtt ccatacagcc tcatgatctg caaaaatctg gttctcctgc
     3601 tgatacagat catccagtga agtgttggag ctgtctgcgg atactgtgcc ggtttccgtc
     3661 agagactcat tggttttgtc cgtgccggca gcatccgttg cgccgcaggc tgtaagcaat
     3721 aaagctatgc agagtgttaa aagtgttaaa atagtaagga attttttgtt tgttttcatg
     3781 attatattct cctttaagta atcgtaataa gattattttc ccataaaccg gtagtggatc
     3841 gcatccttgg gacaagcctt tatacaggct ccacaacgta tgcattccgg atggtctgga
     3901 tgcctgcaca catccacatc cattttacat acccttgaac attgaccaca accgatacat
     3961 ttgctgtcct ctaccttaat attaagaagg ctgaccttat taaacagcga gtagatcgcg
     4021 ccaagaggac agatccattt acagaagggt ctgtaaaata aaatacttaa cacgacaact
     4081 gtgatcaaaa taaagcattt ccaagtaaag agttttccaa gtgcggaccg tatggctgca
     4141 tttcccaggg aaagcgggat ggctccctcc agaacgccct gtggacaaat atatttacag
     4201 aagaaagggt cgcccattcc tatagagttc gttacgagca gcggcagaag gataacgaaa
     4261 acgataagaa tcatatattt cagatatctt agcggcttca gtctggctgt ggaaaatttt
     4321 tttcccggga ttttatgaag taaatcctga aaccatccaa acggacacaa aaaaccacat
     4381 ataaatcttc caagtgtcac acccagtaaa ataaaaaatc ccgtgacata atatgaaaat
     4441 ttaaatcggg aggatccgat caccgcctga aatgcgccaa tggggcacgc tcccgtggca
     4501 gcaggacagg aataacagtt taatcccggt acacatacgg tcttagcgtt tccctgatat
     4561 attttgccct taaaaaaatt agggatgtga atattagtaa gcagtgtggc agcagcctgt
     4621 atccatccgc gtatcccggc tgtctgccct gacagtgatt tttttctctt atccaattcc
     4681 gacacactcc agacataatt ttattgcttt accaagcacg acagcagctt cgcctctgac
     4741 tgcaccgtag ccgatcatag tgatcccggt gacgagcaga acgatctgag atatttttct
     4801 gcacttcaag ttattgttac tcctttcgat cgtggtattt attgaccagt tgacttcgct
     4861 attatacaat aattgttgta aaatctttgt taaggcggga aggaattttg agattattca
     4921 ttgttgagga tgaaaaagaa atatgtgata cagttgcgaa aagtctgtat gacgcaggat
     4981 atgaggtgga tacctgctat gatggggaag aagctcttgc aggtatcctg acggagaact
     5041 atgatctggt cgttcttgat ctgaatctgc ccggcatgga tggaatggag atcctgaagg
     5101 aactcaggca ggaaaatgag gagacaaagg ttcttatatt atctgcaagg gggcagatag
     5161 cggataaggt tgaggcgctg gatgcagggg caaatgatta tatggaaaaa ccatttcatt
     5221 tacaggagct tgaggcacgc atcagaagcc tgaccagaag gaagtttgtg cagaaggatg
     5281 tatgtctgga aagcggtgag ataaagtttg ataccgtaag acgtgaggct tatgcaaaag
     5341 gagaaatggt atctctgaca agaaaagaaa atggtattct ggaatatctg cttctgaaca
     5401 tgggcagacc ggtaagtcag gaggaattga tggagcatgt ctgggatgca tctgtggata
//
LOCUS       DAWEJW010000140         5441 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4474_length_5441_cov_66.993130, whole genome shotgun sequence.
ACCESSION   DAWEJW010000140 DAWEJW010000000
VERSION     DAWEJW010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4474_length_5441_cov_66.993130"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1199 a         1519 c         1223 g         1500 t
ORIGIN      
        1 taactttcca gttacagcag ttccttcagc atctggttga cgctggcggg gtctgccttg
       61 cctttcatgg ccttcatggt ctggcctacc aggaagccga tggctttctc tttgccgttg
      121 tggtaatcct cgacagactg ggaattagcg gcaatgactt cctccacgac tttacgcaat
      181 gcgccttcgt cattgacggt ctttagtccc ttctcttcca catactgttc gggatctaca
      241 tcttcctcaa acatcacccg gaatacttct tttgctacag aagagttgat gactttgccg
      301 tctaccagcg tgatcagcct gctcagatgc tccggtgaga accggatgtc ctccggctcc
      361 atctcttttt cttttaacag gcgcatagtc tccaccatca gccagttgga caccttcttg
      421 ggctggcttc ccagtgccac ggaagcttca aagatatctg ccagatgctt ggagccggtg
      481 atgatatcga tatcatattc cggaatatcg tattcttcct tgtaacgctt catcttctcg
      541 gtgcggaatt ccggctgtct gttgcggatg ctatccagcc actcatcgct gatatagacc
      601 ggtaccaggt cgggatccgg aaaataacga tagtcctgtg catcctcctt ggaacgcatc
      661 gcgtaggaat attccttatt atcatcccag cgtctggtct cctgaatgac ctctttgccg
      721 gcctccagca gatcgatctg tcgttcccgc tccccctgaa tggctctggc gatggccttg
      781 aaggaattca ggttcttcat ctcggtacgg gttccgaact tttccgctcc caattctctc
      841 acggacaggt tcacgtccgc acgcatggag ccttcctgaa gcttacagtc ggacgctccc
      901 agatactgga tcagcagacg taatttttcc agataagcaa taacctcctc tgcactgcgc
      961 atatccggtt cggatacgat ctcgatcaga ggcacaccgg agcggttgta gtccaccagt
     1021 gtgcaatctt cccagtcgtc atgcaccagt ttaccggcat cctcctccat gtgtatctcg
     1081 tggatacgca ccggtttcgt gtaagtgtta ccgtcctctc ccgctacctc gatctctacg
     1141 gaaccgttgc ggcagatagg cagatacaac tgagagatct gatagttttg agggttatcg
     1201 ggatagaaat aattttttct gtcaaatttg caatactgtg taatggtaca attcgtcgca
     1261 agacctactg ccaccgcata ttctaccacc tgcttattca gcacaggaag agatcccggc
     1321 ataccggtac atactgggca ggtgtgggtg ttgggtctgc cgccgaacgc cgtggaacag
     1381 ccgcagaata ttttcgtctt cgtggcaagc tctacatgca cttccagacc aattactgtt
     1441 tcatattgtt ttgccatgtc gcttacctcc tctgctcata ggtataggct gcacggatca
     1501 gtgtcttctc tttgaagcag tcaccgatca gctgcatgcc gataggcagt cccttggaat
     1561 ccgtgccgca gggaacgctg ataccgggca gtcccgctag gttgatggag atggtgtaga
     1621 tgtctcccaa atacatcttg ataggatccg ccagactgct gcccagcttc ggtgccgtgg
     1681 tgggtgccac cggcccaagg attacatcgt attttgaaaa tgcctcatca aaagccttct
     1741 tgatcaatgc ttttacgcgg agtgccttca gataataagc atcataatag ccggaactta
     1801 acacgaagga gcccagcatg atacgacgct tcacctcggg tccaaagccc tgggaacggc
     1861 ttctcttgta catggtatgc agatcctccg catcctgcgc acggtaaccg tattttacac
     1921 catcgaaacg ttccaggttg gagctggcct ctgcagccgc gatcgtatag taagtgggaa
     1981 tcgcatagtc caccagactt aaatcgaact cctccacttc tgccccctgt gctttcaata
     2041 cttccgctgc tgccagtacc gcatctctta cctcaggatc cagtccttcc ccgaaatagt
     2101 ctctgggaat accaatcttc attcccttca catccgctac cagtgcggag gtgaaatctg
     2161 tgttctcccg cttcatggaa gtggagtcct tagggtcatg ggaggtgatg gcctccagta
     2221 cggtggcaca gtccgtcacg tccttcgtca gcggtccgat ctggtccaga gaggaaccat
     2281 aagcaatcag tccgtaacgg gataccgttc cgtaggtggg cttcatgccg acgactccgc
     2341 aataagatgc gggctgtcga atggaaccgc cggtatcgga acccagtgct gcaaagcatt
     2401 cctgcgctgc tactgccgct gcagagccgc cggaggaacc gccgggcaca tgctccgggt
     2461 ttctgggatt ctttgtcact ccgtaataag aagtctccgt ggtgctgccc atggcaaact
     2521 catccatatt ggtcttgccc agaatcactg cgcctgcctt ttccagattc agcactgctt
     2581 ccgcactgaa ggtcggcaca aaattctcta agattttaga agagcaggta gtcagggttc
     2641 cctcggtgca catattgtct ttgatagcaa agggaacccc ggccagaggc ccggtcagtt
     2701 cgcctgcttc gatttttttc tgtacttccg ctgcctgctg caatgccttt tcccgttcta
     2761 cggtcacata gcagtggtag gtatcttcgc tggctgcgat cttatccaga acggcctgcg
     2821 tggcttccac cgccgtagtc ttaccttccc ggatcgcctt agccagctct actgctgtca
     2881 gggataaaat atccatatcc attctccttt tttaatgtct gatggaaaac cattaccgcc
     2941 taaatgctgt aaccagatta taaagcggta ttctacccga tttctaatca aaagtctttg
     3001 gtaccacaaa catgttgtcc ttctcgccgg gggcattggc cagggtctgt tcactctcat
     3061 ccccattggt caccacatcc tcacggaaga cattctgaac cgggaataca tgagacatag
     3121 gttccactcc cgttgtatcc agttcgctta gtttgtcgat atagtccagc attctgccca
     3181 tatccttttt cgccgcttcc ttctcttcat cagaaagctc aagttttgcc agaatgccta
     3241 catattctat ggtctcgtca ctgatcacgt tcgccatact aatttccatt cctttcctgt
     3301 tctttccgca ctaatctctc actttaatgt gtacctcacg cagctgctgt tccgtcactt
     3361 cgctgggagc cccgcacatc agatcctgtg cattgccgtt catggggaag gcgatcacct
     3421 cacggatgtt atcctcgttc cgaagcagca tgatcatacg atccacgccg ggagccattc
     3481 ccgcatgagg gggggctccg aactggaacg cctgatacag tgctccaaac ttggtcttta
     3541 atgtctcttc gtcataacct gcaatggcga acgctttttt catgatctcg atgtcatggt
     3601 tacgtactgc accggaggac agttccacgc cgttgcatac gatgtcgtac tgataagcca
     3661 ggatctccag cggatccatg gtgttcagtg cttccagacc gccctggggc atagagaacg
     3721 gattgtgggt gaatccgatc tgcttcgtct cgggatccag ctcaaacatc gggaaatcgt
     3781 tcacatagca gaaacggtaa gcatccttct cgatcagatt cagtttctcg cccagctcag
     3841 tacggatatg accggcatag tagttcgcct tatcctcttt gtccgcaata aagaagatgg
     3901 tatctccggc cgaaagtcct gccatttctg ccagttctcc cttcatctct tcgggaataa
     3961 acttatcgat gggtcccttg tagctcatat cctctgccac ttcgagataa ccaagtccac
     4021 ccataccaag agaagttgca aagcttagca gcttctcatg gaagcctttg gacatctccg
     4081 catgtacctt gatggcacgg acggttctgc cgataaacgg cttaaacgta catctctgga
     4141 aaaactccgt cacatccata attctcaaag gattgcgcag atccggcttg tcacagccga
     4201 actccagcat ggcctgtttg taactgatga tggggaacgg tgtctcagtg atcctgctgc
     4261 cttcgggagc aaatttctca aaggtatcat gcaatacttc ctcgccgacc ttgaagacat
     4321 cctcctgggt cacgaagctc atctcgaagt ccagctgata gaactctccg ggagatctgt
     4381 ctgctctggc atcctcatca cggaagcagg gagcgatctg gaaatactta tcgaagccgg
     4441 aagccataag cagctgctta tactgctgcg gtgcctgggg cagcgcatag aattttcctt
     4501 taaattttct actgggaaca atatagtctc tggcgccctc gggagaagaa gcacacagga
     4561 tcggggtctg gatctccaaa aatcccatgt ccgtcatctt ctgacgcaga taactgatca
     4621 ccttggaacg aaatacaatg ttgtccttta cttttgcatt tctaaggtca agataacgat
     4681 attttaatct cacatcttcc cggatctctt tagacgtctg gatctcgaaa ggaagctgtt
     4741 tatatactct tcccaacaca tcgatcttgt gtgcttccag ttcgatggtg ccggaaggaa
     4801 tcttcgggtt gtaggtttct tcgtctctgt gctccatgag acctgttatg gacaggcaca
     4861 tttccttggt gatgcccttt aaaagctccg gcttacggat gaccacctgt aatacaccgt
     4921 acatatcccg cagatcgata aaggatacgc cgccgtggtc acggatgttc tccacccagc
     4981 ctgccacctg caaagtctgt ccgatctgct gttcactgat ctctttcatt gttaaatctc
     5041 tgtacatatc gcacctcctg ttatatagtg tcccgaatgt gcgctccctg tacagcttat
     5101 ttttgaatac aaaaagcccc gggacatgtt aaaacatatc ccgggacgaa tcatctaatc
     5161 cgtggtacca cccgaattga caggcgcctt tgcctatcca ctcttgtcac ttaacgcgtg
     5221 caacgtgtca tcctagctac atgtgatatt ctgcagttca gatcacctgc tccggagtgt
     5281 tcccttcgcc agcttccgca aacagcgctc tcagtcggtg acgctgtatt cctgtcgttt
     5341 cccttggcaa gagtctcttt catagcattt acaaaatagt ttttcaactt aggtttataa
     5401 tattcgcaca aatcaaatct gtcaagcgtt ttttattgta a
//
LOCUS       DAWEJW010000141         5435 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4483_length_5435_cov_61.583829, whole genome shotgun sequence.
ACCESSION   DAWEJW010000141 DAWEJW010000000
VERSION     DAWEJW010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4483_length_5435_cov_61.583829"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1524 a         1380 c          967 g         1564 t
ORIGIN      
        1 catttgctgc ttcccatcat tatatccaac ctcgtagcca gccgtataac cttcttcgta
       61 gttcgcatca tgcgtatctg aactttgatc attatttcca caaccagcca atgaaaaaac
      121 caacacaaga caaagaacca ttgcaataat ctttttcata ctgtttcctc cctcaaattc
      181 cgatttagtt caattcactt ttatcatata aaaaagcctc cctttcaaat attattatat
      241 ccaaaaggga gagctttttc cattaatatt ttcccttttc ggaaaagtgt tcccgctgtt
      301 catttcccca attttagtgt atattcatac atttcttaat ttttcgagca atttgagcaa
      361 ctgtatcttg gattttgcct actgaatatt gagaaaattt aggctgagaa ttgaaaaaag
      421 gctatccctt tattaagaca gccttttgaa tttaatttga cttatctatc gtttattctc
      481 tcattcagat tcttctctgt attcaaagac gaaccacttt tcattcatat aaatttctat
      541 tgtatcgtct gccccatact gataaccaaa tccacttcca aaattagact gattatcttc
      601 tgttggtatt tcagttccat cgacagttga tgttatttct ccatccatag tgccacaacg
      661 tgcatccatg atgctttctc tccctgtatc gtagtataat ttaccatcta ttcttaccat
      721 aggtattcta tcccacatgg catttgcttc tgtctgttct atcacggtaa ttttataaac
      781 ctctcctaat tgcgcaggat atgattccat tatttctccg ttatagctaa tttctacgaa
      841 atctccaatt tgcaattcaa ggttctcttc gtttgatata taaaacttat ctgctgaatc
      901 aagttccaaa gaaccatcta ccggttttac tattattgaa tcatgattcg tttcaattac
      961 cgatgcttga aatgttacca ttagcggtct attatctgcc gaagcaagca tttcagttat
     1021 acaattacct aaagcaggcg ttggtttata aattccttga tatggctgtt ctacatattc
     1081 aattccttta tctacataat aaaaaagagt agttcctgcc gccccatcac aatttatatt
     1141 gatcgtaata gaatctttgt tgaccggtgc atcattgatt gattgctgac tggttgtttc
     1201 catatccatt agcatagata gaaattcatc aataaactcc gtagcttcac cttctgtgtt
     1261 aggtgacata gcatatttgt caccgtcaga gacaccaatc gatacaatat cctctctggc
     1321 tggcaatcta ataggattcc cctgttcttt tcttccacat gcacataaca taattgaaac
     1381 aaagtagcaa agacaaacaa tactgcataa tttctttttc atggtcataa tcctccccaa
     1441 aatatatttc gtattatatg ttatctaagt gtatatttca atgtgtggga aatagccgga
     1501 acgtgttttt atgcattgtg aatgcatgtt tataggctcg ttttcagtgg gtacacaatt
     1561 ccgaaaaggg agttaatatt ttactattct tccacctgcg ccgccgtttt cccggtgtac
     1621 tgcacagcaa tacgattctc gggatgcgcg tccgtcaggt acagcacaca caggaagatc
     1681 cattctaaag gtttcatgat aaaatacacc agatccgccg tgtatttact gtggatcagt
     1741 ctcgccacag ggaacccgta agtatcataa aaatgacgca gtgcccggtg gaagccggga
     1801 gtccgttctt ccaacacctg ttcaaaagca ttggcgatgc acaactgacg gttcacaatg
     1861 acttcatgcc catgccgcct ccccaggcgg ataggcttta cgaccttttc atgatcgccg
     1921 gcagctaccg tgcagagata atgttcatca tacatgacat tctgcggtgc ctgcctgaga
     1981 gacagattcc agtcgctggt ctccgtgaat gctttaatca cagaatccgg tgcctgacca
     2041 aacagcatga ggattgcgat caggatcccc aacagtggga acatcaatac cagtcccagc
     2101 accggccacc atgcagcctt ccgcaatatg ctgtcacacc aacgcagcat gggattctga
     2161 taaatcttcc ccgtcccaaa gtgttccgcc tccgcattct gcaccagtgc attccactcc
     2221 cgtattttct tcagaagcag tcgcaccact agcagcacac agcacagtgg aaataatagc
     2281 aaatatccat caagcaaaat cgtcggcttg aatatctgta ctgtgaacag gatcagctcc
     2341 agcagtccca ggtacattgc ggaaatcgac aggactgtca caaggggcgg cagcttccga
     2401 agcggaatgc tctgcagaat ctcataccct ataaatgcca gcagcaggag tactataaaa
     2461 gtaaccatgg aaccactgta aatcggtgta tgtttattgg aattgcttaa ctgcgtctgc
     2521 cagtcagcag atgtcacatc cataatcaac cctaaataac aaatttcata aaatccaccc
     2581 agccaaaatg cgtgctggtc aaacagccag tctcccttca ccgggtgccc tcgttcctca
     2641 cagatcaggc agattacttc actgatcgtc agcaccacag gaaataccag taccgctccc
     2701 attaaaattg cccaggtaaa catttctgta aaagccttaa tgatctccca tacataatct
     2761 ctttgctcat aatgaataac agaggctatg atcgtaaaaa gcgcatagcc cagtccgata
     2821 aatatagaaa tcagccatgc aagcaacacc ggatgctttt tataaaactt tattttttcc
     2881 gtattcattt ctttattttc catcgattct tgcctctatc tgtttccgca tcatatatct
     2941 ggaaatatta aatgtccgca gagatgtctg ctccaggcgc ttcttattat ggttcagata
     3001 ataatagccg aatcctttcg gctgataact cccccaccac tgagcctgcg tatcttcgct
     3061 cataatatcc gtagggttct ccagttcata ggcaccaaaa tcatacccca ggctctccat
     3121 ataaggaata atagccggtg ccgcatccgc actgagatca ctcagataag agtagtcaat
     3181 atattccgct ccccggaaaa acggtccggc gaaaaaatcg tctccgtaat cctgtgccac
     3241 agattcttcc cagcccgtac cgtccgcgcc gggtgcattg gcaatattca ctctggctat
     3301 aatatagtca ggatggctga aggacaatgc cagatacagc acggtcacta tcacgacacc
     3361 atagcggaac agagggaagc tttccctgta aatgttgatc agtactccga taaacatgac
     3421 gaataatacc gccagcatcc acaatacgaa gatccgcagg aaagtcagat aataataccg
     3481 gatgtaaatg atcatacgca ttacgctgga tgcgatcatg ataaaagtac ataatgacat
     3541 aatcgtcata attactttta gtaccttact ttccctaaaa aagctgaggc acaccagtac
     3601 caggatcaga ttcagaatgc tgacagccag cagctggaag aagccctctc ttgcgtactg
     3661 tgcataggta tagccctccg gcagctgcat tttccccaag aacaatccga agatctgaat
     3721 accggaaaac aacagataca aaagtgacag caatgacata atcgtaattg ccagtaccgg
     3781 ctcacccttt ctgcgatcct tcacttcttc tgggatgctt cgcttgcaca ggtaggaagt
     3841 cagtgcatag gaagtaaaga acagaaatgt tacccggatg actacgttga atatattgcc
     3901 cgggcggatc cagttcatga aatccgttgt catcttacgg aataccgcat cggcagagct
     3961 taacagtccg gcagcgatca gcacaatcgg caatgcgatc actactccca acagcccgta
     4021 ccataccttc ttatttactt ttcccttttc acgaaaatag gctttgccat cggaaaaagg
     4081 cttccccagt tccccaaagc tcataaccac caattggcag atactcccca catatttccc
     4141 aagcttccac tttgtggtat caaaatattg attcagcagc agacacatta ccagcaaaaa
     4201 gaccgccagc ttattcaggc tgatgatcgc ccatccgtcc gtgcagaagg tggatatccc
     4261 cagcagcaga atgctgatca tgtaaaaggt gcttcctttt ttcaaggtaa tctccagttt
     4321 tgacagcgag aagacaaaat acagtaaagt gcctgcgaga aaaaaaggga aggtgattcc
     4381 cgatccgtta tgaaacatgc aaaacgcata gaacacggca tagagaaacg tcaccggtcc
     4441 aaagaaatta aaatgttcct tcatgcgctt tgtctccgca gtctctgtct tttctaccac
     4501 ccggactaca ttctgcccgg atatctgtcc ctgatttgct gaagttgttt cctgtactac
     4561 tgtattttcc tgcattgtga tctccccctt tcaggattta attgtttttc tcttcttttg
     4621 aatcttccac atattccaga atgtctccgg gctgacaatg cagcacctcg cagatggatt
     4681 ccagcgtaga gaaacgtatg gcttttgctt tattattttt caggatggac agattggcct
     4741 gggtcagatc cacctgattc gccagttccg tcaggctgat cttccgcttt gccatcatta
     4801 catccagatt tacaatgatt cccataatag atgcctttct acttttgaat ttccgttcag
     4861 ccggcgtcat ttacatagtc gcgtatcaca cgctctatca cttcgtgact gaatttttag
     4921 attgttaagt cgttttcttt cttgtagcgc acggcttctt caaataccat cgccagcact
     4981 ttgctgaaca tgcctgcaat aataaatacc aaaatgatca ccagcatggc aatggtcagg
     5041 tacaccacac atctcacagc gctcatggcc gcgatgaaaa agcttaagtc tcccattctc
     5101 ctaaggctcg ccacattttc cggtacaaag cagttctccg caagcaccgt gccaaacatc
     5161 ttgcgcagtt cattgatcaa caataacgcc agcacaccca gaatggcgaa tatgatcact
     5221 gccggaaggt aatgttcctc tacagcctcc agatattttc ctatgtattt gagactgagc
     5281 ggcagactta ccgtcaccag tatccccgca tagaacatga aatccagcag gtacttcgtc
     5341 ccctttgcca gagaaaactt cctctcccgc agctcctttt ccgacaactc tctcttctcc
     5401 ataatgaata cctctttcct tcttggtagc cttaa
//
LOCUS       DAWEJW010000142         5393 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4530_length_5393_cov_57.223117, whole genome shotgun sequence.
ACCESSION   DAWEJW010000142 DAWEJW010000000
VERSION     DAWEJW010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4530_length_5393_cov_57.223117"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2630..2729
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1299 a         1266 c         1422 g         1306 t
ORIGIN      
        1 atgcgcgagc gcccatcgct gtcttcacta tgcctatgcg cggctcattg ctcgcataac
       61 tttctagtta tttccggccc gggcgcagat tttccagcac ttcatccggc acatacagct
      121 tgccccagcc ggtgccgagg tcgatcttgg gcttattggc accatggaag tcggagccgc
      181 cgctgatctt cagatcgtat ttggaggcaa gcccccggat ctggcgttct tcagcagtgt
      241 tgtaggtgct gtagatggct tcgataccta cgaggccgat gctctttaat tctgccacga
      301 ggttatccaa tcgctcgtcg ctcatatggt agaggatcgg gtgcgccagc acgggaacgc
      361 cgccggcacc aaggatcagc tccaccgcct gagcgggggt caccttttct ctcggaacga
      421 aacagggaca gtggtcgccc acatagcggt caaatgcttc cttcatgctc tggatatagc
      481 cgtgggagag cagatacctt gcaaaatgcg ccctggtgat aactgcaccg gggaattctg
      541 ccagaagcgc ttcataggtg atatcaatgt catgttctcg aaggagtgcg cacattttct
      601 cgttgcggtt ctcccgggaa tggatgaaat cctccaggta atgagcaaaa tccggctggt
      661 ggtaatcaat gaaaagtccc accatgtgga tatcttttcc ctggtattct gtggaaagtt
      721 ctataccggg gatgacttcc ggcacctgag gcgcgggagc gtcagccggg gatccttccg
      781 cataccggat cgcccggtcc aatccgttta cggtatcatg atctgtgagg gcgaaggctc
      841 gcagtccctt tttcatcgcg taatcgacta attcctccgg ggtgaaagtg ccatcggagc
      901 aggtggagtg tacgtgtaaa tctaccataa tagtaaagct cctttcaaaa aatggatgtc
      961 ggcattaccg gcatccattt tatttgtctt agttctcgtc gtcctcggtc tgtgcagtga
     1021 atacagtacg ctttcttgcc atttcatcgc ttgccagata ctcatcgtag gtagtgatct
     1081 tgtctaccag gctgccgttt ggcaggatct cgatgatacg gttggcggtg gtctgtacga
     1141 actggtggtc atggcaggag aacagtgcca caccggggaa cttgatcaga ccgttgttca
     1201 gtgcggtgat ggactccatg tccagatggt tcgtgggctc atccaggatc aggcagttgg
     1261 caccgctgat catcatcttg gacagcaggc agcgcacctt ctcaccaccg gagagaatac
     1321 gaaccttctt cacaccgtct tcaccggcga acagcatacg tcccaggaaa ccacgtacat
     1381 aagtggcatc cttcacggga gagtaaccgg tcagccagtc ggcgatggtc atatcgttgt
     1441 cgaactctga ggtattgtcc ttcggaaagt aggactggga agtggtaaca ccccacttgt
     1501 aggttccctc gtccggctcc atttccccgg ccaggatctg gaacagagta gtcttcgcca
     1561 gctcgcagct acctacgaaa gccaccttgt cttcacggcc cagagtgaag gaaatgtcat
     1621 ccagcacctt ttcaccgttg atggtcttgg acagatgctc cacagccagt acctcgttac
     1681 cgatctcacg ctcgggacgg aagtcaatat aaggatattt tctgctggaa ggcttaatat
     1741 catccagctc aattttttcc agtgcacgtt ttctggaagt agcctgcttg gacttggaag
     1801 catttgcgga gaaacgggag ataaactcct gcagttcctt gatcttttct tcctttttct
     1861 tgttggcttc cttcatctgc ttgatcagca gctggctgga ctcgtaccag aagtcgtagt
     1921 taccggcata cagctggatc ttgccgtagt cgatgtcggc gatctgggta cataccttgt
     1981 tcaggaagta acggtcatgg gatactacga taacggtatt ctcgaaatca atcaggaaat
     2041 cttccaacca tgcgatcgca tccagatcca gatggttggt cggctcatcc agcagcagaa
     2101 tgtcggggtt accgaacagt gccttggcca acaggacctt caccttcagg ctgccgtcca
     2161 gatccttcat gagagagtag tggaactcgg tgccgatgcc aagaccgttt aacagcatgg
     2221 cagcattgga ctctgcttcc caaccgtcca tctcggcgaa ttccgcttcc agttcgctgg
     2281 cacggatacc gtcctcgtca ctgaaatctt ccttggcata gatggcatcc ttttccttca
     2341 tgatctggaa gagacgttcg ttacccatga taacggtatc cataaccacg ttgtcatcgt
     2401 atttgaaatg atcctgttcc aacacggaga gacgctgacc gggggtgatg acgatatcac
     2461 ctttggtagt gtccagctga ccggacagga tcttcaaaaa ggtggattta ccggcaccgt
     2521 tggctccgat gagtccgtaa cagttgcctt cggtaaactt gatgttgaca tcttcgaata
     2581 atgctttctt acccacacgg taagttacgt tgtttgcact gatcattctn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ttgcagtggg aatcctcgtg atcaccatcc
     2761 gggaaaataa gctgcgggaa gaagtggaaa aggcagaagc cgcaaacgca gacactatag
     2821 aagaaaaagc gatggccgaa ggcgtggaag ctgccggaga gatcgaggca gtgggagaca
     2881 ccggagaggc aaaagctttc gaggaaaaac agcagacggc aaagaccgaa agcgccaagg
     2941 acatgccgaa ggatgtgaag cgcagtatgt acttcatgct ggcgtccatc ttcttatggt
     3001 tcaccgctta taatgccgtg accacagcat tttcccgtta tacgaaagta gtatggaaga
     3061 tggagggcgg aagcttcgcc aactgtctga tggtggctac cgtggcagcc attttcagtt
     3121 atatccctat cggaagtatt tcagcgaaga tcggccgtaa aaagaccatc atgggcggtg
     3181 tcctgctgat ggcagcctgc tacggagcgg ctattttcgc cagagaatat caccccatcg
     3241 tcaatgtggc ctttgccctg atcggtgtgg catgggcagc gatcaacgtg aattcctatc
     3301 ccatgatcgt ggccatgagc agtggcagag atgtgggtaa attcaccgga acctattaca
     3361 ccttttccat ggcggcgcag atcctgaccc cggtattgtc cggatttttg ctggagaagg
     3421 tgtcctataa cactctgttc ccctatgcgc tgttcttctc cctgatggca ttcctgacca
     3481 tgacccaggt acgccacggc gatgtgaagc cccagaagaa ggagagcatt ctggagaact
     3541 ttgacgtgga cgattaataa aatagacaat aaatcgttag aaaatcttta gaaatagcta
     3601 cttgaaaatt gcggggcagt ggattaaagt aagatgcagc agcttttcgc ggagactaag
     3661 agggcgcaga aaagatgtat gtgaatcaaa acataagata gaaggttgaa ttgagaatga
     3721 aagttttagc aatcatactg atcgtcattg tagtgctggc gattctttac ttccttatga
     3781 tcatgccccg gatgatccat aagcccgata cggccccttt tacagaatgg ctctatgccc
     3841 acagaggcct gcacgataat gcaacggaag caccggagaa ctccatggcg gcattccgta
     3901 aggctgcgga tgcgggcttc ggtattgaac tggacattca gctgacgaag gacaagatcc
     3961 ccgtggtgtt ccatgatttt acattaaaaa gagtctgcgg cggcgaaggc aagatcagtg
     4021 attataccta cgaggaattg cagcagtttc acctgtgcga ttctgtggag aagattccga
     4081 agtttgagga tgtattgaag atggtagacg gaaaagtgcc tttgatcgtg gagttcaaga
     4141 tcgagcgcac cgacctgtct ttgtgtcccc ttgcagataa aatgctgcgg gcctacaagg
     4201 gaatgtactg catggagtcc ttcaaccctc tgggtgtgcg gtggtatcgg aagaaacatc
     4261 cggatctggt gcgaggccag ttgtccgatg cttttctgaa agaaggagag tatgtcggga
     4321 tattatattt tgtattgcag aatctgctgc tgaacttcgt gaccaagcct gattttgtgg
     4381 catacaatca tcattatcct gaaatcctgt cccggaggct ttgcagaggt ttgtaccata
     4441 atactgcagc cgcatggacc atcaagagcc agaaggaact ggacgaagcg agaaaacatt
     4501 tcgatatttt cattttcgac agttttgtac cggaagcggg aaagtagaga aaacagcata
     4561 aaaaggtcgg ttccatgctc cccaggaaac cggcctttta ctatatgcga tatgcgagga
     4621 aaaaatatgt ataccaaaga agaattgaaa cagcagttac ggaatgtggg acttacggga
     4681 aaagagacga tcctgatcca ttcttccatg aaggccatcg gagaagtgga gggcggtgcg
     4741 gatatcgtgc tggatgcctg gatggaatat tttgcggaag gccttctgct attgccgacc
     4801 catacctggg ccaatgtgaa tgcacagcac ccggtgtatg actatcgtaa taccccatcc
     4861 tgcgtgggac tgctgaccaa tctgttccgg cagagagagg gtgtggtgcg ctccctgcat
     4921 cccacccatt ctttagcggg gtacggaaaa aatgctgccg aatatctggc aggagaggaa
     4981 gaacgtaaca ctccatgtac cccgggagga gcctacgacc ggctgaaaga ctgcggtggt
     5041 aagattctgt tggttggtgt tggacatgaa cgcaatactt acatccacag tgtggaagag
     5101 gtgctgaatg ttccgaatcg tctggcggcg gagcccatgt tattgacaac gatcctgccg
     5161 gacggaagag aaaaggaagt atatatgaga aaacactata atcccgacca accccatatc
     5221 tcggaagatt ttgtgaaatt gaaccaggcg tttctggatt gtggagcagt ggaagaggtg
     5281 atgttcggag atgcgaagtg tctgctgtgt gatgcgcaga aagtgttccg ggtggtacgg
     5341 catgtgctgg caccggatcc ggaatgtctt gtgacgaaac cggagatttc tgc
//
LOCUS       DAWEJW010000143         5340 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4567_length_5340_cov_70.004163, whole genome shotgun sequence.
ACCESSION   DAWEJW010000143 DAWEJW010000000
VERSION     DAWEJW010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4567_length_5340_cov_70.004163"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1553 a         1078 c         1420 g         1289 t
ORIGIN      
        1 gcacagatca aggaactggc ccgttttttt gcacagatcg ttgattacaa gtcacccttt
       61 accagcaccc attccattgg tgtggcggag gatgcggagc gcctaagccg ctatatgggc
      121 tttgatgaag agaccgtgca gaaaatgtac ctggcgggcg ctttacatga tatcggaaaa
      181 gtggcggtag gtaatgagat cctggagaag ccgggaaggc ttacggagga cgaatttgct
      241 gtgatgaaac atcatgcggc atatacctat tatattctgt ccggcgtaga tgattttgac
      301 gagatccgcg actgggcggc attccaccat gagcggctgg acggaaccgg atatcctttc
      361 gggaagaccg ccgcagaact gaacacgcag gagcgcatga tggcctgtat agacatctat
      421 caggcactga cggaaagccg tccctataaa cagggcatgc ctcacgaaaa agcttgtgag
      481 atcctgcggg atatggcgaa taagggctgg ctggatgata ccatcgtgaa gcaggtcgaa
      541 gactgcttcc ggggataaaa gatgtgaggg cgattattgc aggattaata tgacgcagat
      601 atagggaagg tataaagata tggacaactt tatagaagca ttgtatggac aaccggaagg
      661 aactattccg gaggagtatg attggtttgc tccgctgttg ggtgattggg agtttgatta
      721 taaagataag tttatagatg gtgatcataa caggccacag cgtcaattaa agggagagtg
      781 gatctttagg cgggtattga acgggatagg cattgaagat atgttcatat gtccttccag
      841 agcaacgagg gagacttctc cacagccgga tggtgagtat ggtgttggac tcagaatata
      901 taattcaaag acccattgtt atgatatggt atatgcatgc aagagtaaat ttacaaattt
      961 gtgttttacc atggaaaatg gaatattgac gggaactgtt ctggaaaatc ccaatcgaaa
     1021 atggcgattt gtggaaagaa atgaaaatac atttcattgg cagaatatta ccattcagga
     1081 gaatggtgac attactgtaa attgtgaggt tttcgggaaa agaaaaagcg aggaagcgta
     1141 aaacatgaac gttatagaca ttatgaagcg ccctgcggcc tatgccagtg gctatgaaaa
     1201 tatcccggga gaggaacaga accgggcaaa acagctttgc tgggaatata atagaacggc
     1261 tccgagtgaa cagcagaagc ggcgcgagat cttgcaggag cttctgggaa cctgtcatcc
     1321 gatgacgggc attgaaccgg attttcactg tgattatgga tttaatattc atacccacgg
     1381 acttacagtc atcaattata actgtgtcat tctcgatacc tcacccgtca atatcggtgc
     1441 gggtgcattt atcgcgcccg gtgtctgtct ggcctgctcc ggacatgcgg ttgacccggg
     1501 acagcgcagt cagggaatcg gtacttctgc acccatcacg ctggaagaaa atgtatggat
     1561 cggagcaaat gccacggtct gcggtggtgt aaccatcggc gcggattctg tgatcggcgc
     1621 cggaagtgtg gttacccatg atattccggc gggagtgatt gcagcaggcg ttccctgccg
     1681 ggtgatccgt cccattacgg agaaagataa gatccggccg gaggacattc ttttctaaaa
     1741 aagatgacga tagccccact cttgtgttac tatggtggaa aataatacaa aggagtgggg
     1801 agaatatgtt tacaaaaagg agttgggaat ccttttattc cggtctcgtt atggtgggac
     1861 tggtatttat ggtgttcccg gttatttatt tctgcggtgc gacaaaggag aagcagatcc
     1921 tgccgccgct tatcatgttt ctgctgggtc tggtgatctt cgtgattagt gttcactttt
     1981 ctgtcaaata ccacaaggag aaaacgaaat acaccaagta taagatcgac aaatctctga
     2041 cctacacatt caccttaact tacggggata aggtggagac cgtcacggat ttttcccaga
     2101 tcgaatatgc catcaagtgg ctggcgaaat accggacggg ccatgtggag atcgcagtca
     2161 cgcctactat gggaagcctg aaaaagttct acatctgcta cactgacaaa gatccgtaca
     2221 ttcacatgct gatcgagcag gagagagaag acggcgtggg atattgggaa gaacggggag
     2281 attcttacaa gattcccatt atgacactcg agcggctcta tgtgaaacat aagatgattt
     2341 cgtttgatta cctgaaccgc agggagactg gtgtggatgc aagggaggga ctgatcctat
     2401 gatgcagcgc tggcgggaaa aactgaaata tatagaagcc ggaatgatca tcagtgcgtt
     2461 gatccttttt gtggcaacgt tgatattcgg agacaacctt acgctggaag tgatcgcgtt
     2521 tatagtagga accgtgttgt tttcccgtgt gatcgagcgt atggataaaa aagaaatcga
     2581 gaaaatgaaa cgccaggaag aggaagaact gaaagatatt gtctgagaat aaatcctgtt
     2641 tcccaaagga agaatgattg acacggaggt tagcgagtgc taaccttagg aagagatttt
     2701 gaaaactttg ggagtgacaa tatatggatc tggcattctg gctggcgatt gcagcggcga
     2761 tcctgtgcgt tgctgtcgcc atttacggtg aaagtaaaaa ggataaaaaa taggcaaaag
     2821 aatgacactt cagcaattaa agtatgtaat aacggtagca gagaccggca ccatcacgga
     2881 agcagccaac aggctgtatg tttcacagcc cagtttaacc aacgcgatcc acgagttgga
     2941 aaaggaaatg aatattgtta ttttcaaccg gaccaacaag gggatcacgc tttccaaaga
     3001 gggagatgac tttttgggat atgcgagaca ggtgctggag caggcggcga tccttgagga
     3061 taaatacaag ggaaatcccg ggggcaaaaa acagttctgc gtatccaccc agcattattc
     3121 ctttgccgtc aacgcattcg tggacctgat caaacagtac gggcaggaag aatacgattt
     3181 cagcctgcgg gagacacaga cctatgagat catagaagat gtggcgcgtc tgcgcagcga
     3241 gatcggaatc ctgtttttga atgatttcaa tgaagcggta ctcagcaagc tgttgaaatc
     3301 ccatgatctg gaattccacc agttgtttgt tgccagtccc catgtattta tcagcagaaa
     3361 acatccgctg gcgggtaaca gcataattac gaacgaagaa ctggaagaat acccgtacct
     3421 gtcttttgaa cagggagagc acaattcctt ttatttttca gaggaaatct tctctgattt
     3481 tgaacgcagg aaaaatatcc gtgtgcggga cagagcgaca ctgttcaatc tgctgatcgg
     3541 cctgaacggt tacaccgtgt gcagcggcgt gatcgataaa aaattaaacg gaaaagatat
     3601 tatcgccgtg ccactggcag atgagagtga catgcggatc ggttatatca cacacagaaa
     3661 aggaatgctc agcagactgg gtactaccta cctggaagca ctgcagaagt atttaggatg
     3721 aatgggttgc ccatagtttt aaactatgga caaccctcat ttttacgtat tttgcggatt
     3781 tcatcatttc tcttataatg aggacaattc aaaaaacgac aaaacaacaa agaaatggag
     3841 agaatgacaa tgagcaattt acagacacct tttcgctatg atttcgtagg aagtttttta
     3901 agaccggaga agctgaagaa agcaagaaga cagttcaatg aaggaaagat cgatgctgcg
     3961 gcactgaaga aagtggaaga tgaagccatc acagagcttg tgagcaagat caaagagctg
     4021 ggctatcatg tgatcacaga cggtgaattc cgcagagcca cctggcatct ggatttcatg
     4081 tggggattcg acggcatcgg ccatacccct accaagacgg gacttccgtt ccacggcgag
     4141 gcggccatgg tggatgatac ctatattgta ggcaagatcg gactgaccgg ggagcatcct
     4201 tttgtagatc atttccggtt tgtaaaagca ttagaggatg aaaataccgt agcaaagcag
     4261 accatacctt ctcccgccca gtttctggca cagtttacca tgccctttaa ccgaggctgc
     4321 acagagaagc attatccgaa tgagcaggaa ctggtaaatg acatcgtggc ggcctacgga
     4381 aaagtgatcg atgatctcta tgcggcagga tgccggaact tacagctgga tgactgcacc
     4441 tggggcatgt tcgcagacaa gatcggtcat accctgtacg gaaccaccag agagggaatt
     4501 gtggagttcc agaaagccca caaggatatt aacgataagg tgatcgccaa tgcaccgaag
     4561 gatatgatca tcaacaccca tgtatgccgc ggtaatttcc attccaccta cgcaagcgag
     4621 ggtgcctatg attccgtagc agatatcctg ttcggcgaag agaatgtgaa tgcctatttc
     4681 ctggagtttg atgatgagag atccggcggt ttcgcacctt tggccaaggt atccggggag
     4741 aaaaaggtcg ttctgggact gatcactacc aagtctcccg tactggagga caaacagctt
     4801 gtcattgaca gaatccatga tgcggcaaaa tacattccac tggaaagatt atacttaagc
     4861 ccccagtgcg gatttgcttc ctgcgagatc ggcaacaagc tgaccgagga agaacagtgg
     4921 gcaaaactcc gacttgtgaa agaggtggcg gaggaagtct ggggaaaata aataaacgtg
     4981 gttccggcga ttaccagaga ggtggtcgcc ggaattttaa ttatattcaa ttgtctcatt
     5041 tttctggata ggctataata tacctatggt tatccctgtt taacattttc taaatgggga
     5101 tttttgaaat attttgaata tgaaaaataa tacggaggaa gcagaaacat gaataaacaa
     5161 acagcccatt accatcttcc cggtctgttt gaattctatg aattatacag aatgttcctg
     5221 ccgttgtttc gggagcatcg agaatatttt tacgactggt gcgacatcgg ttccatctac
     5281 ggggccccgc cggactgcat ctggggaggc ggccgggtct cactggagga tcacgacgca
//
LOCUS       DAWEJW010000144         5269 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4634_length_5269_cov_53.365171, whole genome shotgun sequence.
ACCESSION   DAWEJW010000144 DAWEJW010000000
VERSION     DAWEJW010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4634_length_5269_cov_53.365171"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1216 a         1316 c         1115 g         1622 t
ORIGIN      
        1 tcttctgttt cttgttcata attacattct cctttttatg tgttttttaa tttcagggta
       61 gtaagttttc cgccccattt attaattatc gttctgtccc tacacagaac ggataatcca
      121 aaattataaa ttatccaatg ctgcatacat agataccata ggtgccatac atgctgcaat
      181 caatccgcct actacgattg ccagtacaat gatgatcata ggttccaagg cagccatcaa
      241 agactgaatt tccatttcta cttcttcatc ataatagtct gcaatcttgt ccaacatttc
      301 ttccgtacta ccggcttctt ccccgatacg aatcatttga tataccatag gtggaaatag
      361 tccactctcc tgcaaaggtc tggacaacgg cataccaatg gtaatttctt ctcttgcatc
      421 ctgtaaggct tctttaaaat agatattact cataactcca gacacaatgt ccacagcttc
      481 aatcaacgga acgcctgcac ccatcagggt actcagagtt ctcgccatca tagatgctgc
      541 agacttcacg gtaagttttt tgaaaagcgg taatttcaaa gcaatcttgc caaatacatg
      601 ctttccggga ttcgttcctg cgaaatattt aattccaatt gccgccgcaa taataaccgg
      661 aataataata aaccaataat tttgtatgcc atggctcatt gccacataaa atttcgtaat
      721 ccagggcaaa tcggctccca actggctaaa catgtcttca taggaaggaa ttacctttac
      781 cagcattaca atcgttacaa taacagctac aatacaaaca accacaggat aaatcattgc
      841 ctttttcagc attgcctgtg tcttgctgtt tttctccaac tgcaccgcca ctcggtcgag
      901 tgctgtttcc agactaccgg atgcttcacc cgcagccacc atgttcacca tgattgccgg
      961 gaacaccttc ggatgttctg ccatggaatc tgccagggtc tcaccctttt ccacgctgat
     1021 gcggacttcc ttcagtgcgt cctgtaatct cttgttctct gtctgctcgc agagcatttt
     1081 aagggattcg aggatgctga cgcctgcttt ggtcatgctg acgaactggc ggcacatgac
     1141 gcttagatcc ctggccttgg gccatccgcc gatctggata tcgatgtctt tcgtcaggaa
     1201 gctctgttct cccagatcga taacggtcag ctcctgcctt ctcagttctg ccattgccag
     1261 atctttgtta tccgcttcca aacttccctt tacttctttt ccagcctttg tgatggctct
     1321 gtagccatag gtcgccatgt gcctttcctc caatgcgtct ctatctttaa ctttctcccc
     1381 aactatgtaa gacaagcccc ggtcctgaaa aggtccaggt ttttgaaaaa tttttataaa
     1441 ttttgataaa tttctataaa atattgcggt ttttatagaa ttatacgtca aatgatacct
     1501 ttaccatctc ggagaaggag gtggtgccgt ccagtacgta gtccgtggca ctcatacgca
     1561 gggtatgcat tccttctttt aatgcttctt cttttaagat atcggcaccc tcccgcttgc
     1621 tgataatggt tttcagcttc ggagtgatct gcatgatctc atagacaccg atacggcctt
     1681 tgaagccggt attgtcgcat ttgctgcatc cacagggctc gtagatggtt acatcttcct
     1741 cttccctcat gcccatgaac tctttttcat ccttagtggc ctgcttcggc tttttacaga
     1801 aaggacagag tctgcgtacc agtcgctggg cgataacgcc gatgacggaa tctgcgatca
     1861 ggtaggattc gatgcccata tcctccagac gggtgatggt gctggcggag gaattggtat
     1921 gcagggtact taccaccagg tgtccggtga tggacgcctg tacggcgatg ctggcggtct
     1981 cctggtcacg gatctcaccg atcatgatga tatccgggtc ctgacgcagg atggaacgca
     2041 gtgccgatgc gaaagtcaag tctgccttgg tgttgacctg tacctggttg ataccgtcaa
     2101 tattggcctc gacaggatcc tcgacggtga tgatgttcac gtcttcttta ttcagttcgc
     2161 tcagtgcggt atacagggtg gtggacttac cacttccggt aggtccggtt accaggatga
     2221 taccgtgagg atttttcagg atatagtcaa actgttttaa ttcgtagggt ttgaagccta
     2281 actggctctt gtcgcggttc agtgcactct ttgcggcaag acgcattacg actttttccc
     2341 cgtatacggt aggcaggatg gatacacgga tatcgtattc cacacggtct accacctggg
     2401 tgatacggcc gtcctgcggc tttctcttct cggagatatc catgccgcca atgatcttga
     2461 tacgggcgat gatggcagac aatacattga caccgtatct cgctctctca tagagggcac
     2521 cgtcgatacg gtaacggaca cggatgatat tttccatggg ttcgatatgg atatcggatg
     2581 cacgctggcg gacggctttt tcgatcatat cctttaccag cattacgata ggggagctgt
     2641 tgatgttctc ctccgtggcg atgtcttcca ccacatccag ctttttctct ttggcgtagg
     2701 ctgccagtgc ctcggttact tcctcctgac cgaaatattt atcgatggcc agcatgatgc
     2761 tgcgggtggt ggctactacg ggctctacct ggaagttggt gatgatgttg atgtcatcga
     2821 tggcattgta atccagggga tccgccatgg cgaccatgag aacgttgaag ttattttctt
     2881 tgtaggcgaa ggggaatatc ttgttcttct tcaggacgtt gaccggtacc agattcagta
     2941 cttccttgtc cacattgacg ctctggagat cgatcatatc cagatccagc tggcggctca
     3001 gtgccttcgc cagatcatcc tccgatacga tgccttcatc taccagaaat tcacccagct
     3061 tcttgccctg acgattgggg ttgtctagtg cctgctgcaa ctgctctgcc gtgatcaccc
     3121 cactgtttac caataattca cctaaccgga ctttttttct gcctaattcc ataaactctc
     3181 tcacccatta cttttgttga taattggaaa tatgtgtgaa attgtagtat tttttggcta
     3241 caactcacaa atattttgtc tcaatacgga aatattatag catattttag atttatgtaa
     3301 attgttttta gtacttttgt ttcaatttct gagaaaaaca tttttgttgc tttctttcct
     3361 gttacacagt acaattattt cattttattt tgaaagtttc taaatacgcc aattcttcgg
     3421 cgaattttcc caacatttat tgacttcacg tattatacgt gttaccttaa gaaaggagag
     3481 ataatccatg cgttttcgcg aattagagcg aatcatcctt gacgatggct gggttttgtc
     3541 tgatatcaga ggcgatattc ctttgcgagt tgttaattct atttttaaac aggctggact
     3601 aaaataatat ggaacagcct ttgaaaggag tgatcttatg aattatatat atccggctat
     3661 tttttacccg gaagcgaatg ggaaatactc tgtcattttt cccgatctta atgatctcgc
     3721 cacctatggt gacagtcttg cagatgcctt tgccatggca caggaagcat gtgcacaata
     3781 tctgtttact tctctgagag acggcgatgc tcttcccgcc cctactgcaa ttaatgcagt
     3841 gaaatcagat gatcccactg cattaataaa tctaatctgt gtgaatttgg ataattatgc
     3901 ccgggcctat gatgacaggg ccgtcaaaaa gacactgact attccggcgt ggttaaacac
     3961 tgcctgtgat aattgcggta tcaattattc caaagttcta aaggacgctc tcattgccaa
     4021 actgcaacaa gtgcaaatgt gaccgtatca caaagtcacc gcgtattcac tgtcatacat
     4081 tttcttcaga ttttctacgc tgttatattt ttcaccgatg cagaattcct tggaccaggt
     4141 catgtagtaa gcccagggga ttctgctttt ttgcagaaga tccacatcgg gaaggtatcc
     4201 cacttctgcc agggcagcca ctttgccacg ggaggtggct gccaccagct tctcgtaggc
     4261 atccgcatag tctgtggcct gatactccgg gaggtagaca tccatggata ctacatccac
     4321 gtattcatct ccggggtagg cttttggtat atcgctgttc catacccaga gaaggttgtg
     4381 caggtctttc tcacctgtgt agtaatcgaa catcaggtga tacaggtttc tcgccacttc
     4441 cgggccctgc gctccccacc agaaccaggt accgtaggat tcatggaacg ggcgccagag
     4501 aatggggatc cgctcttcct gaaagcgccg caggatctcc gcaatcacat ccatgtcatg
     4561 atagaatgcc actctctctg gggttccctc tttcaatact tcccttgcat caaaatccgt
     4621 gtgttcggta tagaagctct tgtctctgcc ttccagaggc gagaaccagt gaaaggtaaa
     4681 ggtgagaata ccaccatttc cggcttcccg ctgttttctg gcccattgca atgccgtctc
     4741 cacggtcccc ttattttctt ccacttcccg gatgcattcc ggggaagcat cttcataatt
     4801 gatattcgga gaatacgcca gcagttcaaa gccccgcagc ttcggttcct ttccggtgac
     4861 ctgcctgatg taatctatct cttcgcaggg aatggtctgg gtatgctgcc cggtgatgat
     4921 ctgctttccc gctacagtat gaagataatt ccataattct actgtttctt ttgttgcctt
     4981 cggatctgct gttgtcatct tttcttcctt ttccattttc atcttcatac cccgcccgca
     5041 aaagcaagag ggtacagaga cttttatttt gtctctgtac cctcagttta tgacattctg
     5101 tcgcttatcg caagtattat tccggcttta cctcgtgata atcggcattt gcgtcagact
     5161 tggtctcttc ggccctctct gcgcctgcag tttccttcgc aaatccctta gccagcatct
     5221 cattgatgtc cagtccggta gactccttga taccatcggt aacctgggt
//
LOCUS       DAWEJW010000145         5249 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4656_length_5249_cov_64.150751, whole genome shotgun sequence.
ACCESSION   DAWEJW010000145 DAWEJW010000000
VERSION     DAWEJW010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4656_length_5249_cov_64.150751"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1145..1244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1325 a         1033 c         1404 g         1387 t
ORIGIN      
        1 cacccaccta tgagcaagct catgtgggct tagacctttt gaccctggca tcataaaata
       61 taaatattat tgtaatggat tcccagggaa acgtaaagag gagaattgct gcagatggat
      121 tttttgaagc aggtgtcaat agaaatatat ccggaaggtg catcggacga ggaacgaaaa
      181 agctacagta aaaaatatgg tgcgcagatg cacgccctgc tggatgcgat tcgcagacag
      241 agacaggaaa gagaattttc ccaacaaaga aacgggtcgg gaaaagagtg cttcgaagag
      301 aaaagcgtcc gggatagcat gatgtccggg tatgaaagcg ggcagggaaa actctggatc
      361 gtagataacg gtatacgtgc acaggaactc ttggagcagg gctgcccggt gcttgtctgg
      421 ctccatgagg acaacaggga tcaggatttt agcggtgtcc gttatgcctg tgaaaatatc
      481 agtgaactgg attttgatta tctggagaag gtctatcgca gatacgtggg cattccctgg
      541 gagattctga ccacggagag gtgtctcatc cgggagaccg gtgcggagga tctggatgcc
      601 ctttatgaga tttatgcgga tccttctgtg acgaagtaca cagaaggact ctatccggag
      661 cgggcgaaag aagaggccta tctgaaggat tatacggaaa atatgtatta tttttataat
      721 tacggcgtct ggacgatctg tgaccggatg accggacagg tgatcggcag agcgggattc
      781 tccaaccgcg agggttgcga ggatccggag ctgggcttcg tcatcggtgt cccgtggcag
      841 cggcaggggt atgccacgga agtatgcaag gcattattaa aatacgggaa agaagaattg
      901 ggatttgagc gagtgcagat gttagtgatg ccagagaacg aaatatccct gcaccttgcg
      961 gaaagactgg gattttccca actggatcag gtcacctggg aaggaacttg ctatgagcga
     1021 cttcttatgg aactgtaaaa atgtaaagaa aatataaaaa acatgttgac actaaaaaac
     1081 tacggtgcta tactctcaac atagcaaagg cgctacagaa taaaactgta gcgccctttt
     1141 tgttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngggtat gtggaaaaca
     1261 gtttaactgc tataccctaa ggatgcgatg caatggggct tcgaaggatg agaccatagt
     1321 atcgcatatt tttttacacc ggtgaaatat tgtggaagat atttcacgaa gagtgaggag
     1381 gaaaatgtta tgacagaaga aatcttaagc gcagtagatg gtcaattatt tgcagtctgg
     1441 ttcctgatcg gtgcggccct ggtgttctgg atgcaggcag gattcgcaat ggtggagact
     1501 ggatttacca gagccaagaa tgccggtaat attctgatga agaatctgat ggatttctgc
     1561 atcggtactg tagtatttat tttgattggc tttggattgt tacttggtga agatgtagca
     1621 ggtctgatcg gcaagccggg actggacatc tttactgcct atgagaattt tgattattca
     1681 aagtttgtat ttaatctggt gttctgtgct accacagcta ccatcgtatc cggcgctatg
     1741 gcagagagaa ccaagttctt atcctattgt atttattccg gtgtgatctc cgctttgata
     1801 tatcccattg aagctcactg gatctggggc ggcggctggc tttcacagct tggcttccat
     1861 gatttcgcag gttcctgtgc aattcacatg gtaggcggta tctctgcttt gatcggagca
     1921 aagcttttag gtccccgtat tggtaagttt gaaaaggaca agagtggtaa gattgtaaaa
     1981 gtaaatgcat tccccggaca taaccttccg attggatgcc ttggtgtatt tatcctgtgg
     2041 cttggctggt atggattcaa tggtgctgct tgtacttccg tagagcagtt aggctccgtg
     2101 ttccttacca ctaccgtagc tcccgcaatc gcaactgtag tatgtatggt atttacctgg
     2161 atcaaatatg gcaaacctga tgtatccatg tgtctgaatg catctctggc aggtctggta
     2221 gcgattaccg ctccttgtga tgtgacggat tgcttcggtg ctatcgtgat cggtgcagta
     2281 gcaggacttc tggtagtatt tggtgtatgg cttctggatt ataagcttca tattgacgac
     2341 cctgtaggtg ctgttgcagt acattgcatg aatggtatct ggggtactat cgcaaccggt
     2401 ttatttgcaa ccacttccgc acccggcaat gacagtgtgg taggcctgtt ctatggcggt
     2461 ggcttcaggc agcttggttt gcaactgtta ggttttgtaa gtgtggcggc atggactgca
     2521 gtaactatta ccattgcttt cctgatcatc aaggctaccg taggcttacg tgtatctgag
     2581 gaagaagaga ttgttggtct ggactcctgt gagcatggtc ttccttccgc atatgcaggc
     2641 ttcagcatta tggatatttc caacactatg accatggagg taaatgaaaa taccagcctt
     2701 ggcgtaagtg attatgacac agcttccgca gccatgaagg aagcggcagt aaaggtagag
     2761 acagctccgg cagcaattcc tgcaaccgga atctacaaag tagtggttat tgcaaagctt
     2821 gcccggttcg aggtactgaa gaaggctctg aacgatctgg gtgtaaccgg tatgaccatg
     2881 acacaggtca tgggctgtgg cgtacagaag ggtgccggcg agatgtatcg tggtgttgaa
     2941 atggatgcga ccctgctgcc taaggtgaag gtagaagtta ttgtcagcaa gattcccgta
     3001 agcgcagtca tcgatgctgc caagaaggct ctgtacaccg gacatatcgg cgatggcaag
     3061 atcttcgtat acaacgttga caaagtagta aaggttcgta ccggcgaaga agacttcgca
     3121 gctctgcaag acgtggagta ataatgccag ggttataatc cctaagacac gacgtgcctg
     3181 cacgggagtg gcttgcccct aacttagctt ttatctaatc cctcaatttg gtatatacag
     3241 ggctgtgccc ggatgctgcg actcatccgg gtgcagacag gatccccggg tgcttccacc
     3301 acccggggat tttcctgccc tgagctgtgg ataagattgc cctcttgaaa aggttctgtg
     3361 gaaatgatac tatcatcgta gaagtacaga ttttcaaagg gcatgattta tatggaagaa
     3421 aatacaaaga aaacacaaaa gcagacagaa aatgctatag ggaaagaaaa caagatcgta
     3481 acgggagtca tctggcagca gctgctcctg ttctttttcc ctattttgtt cggaaccttt
     3541 ttccagcaat tatataatgc ggcagatgca gtgattgttg ggcgttttgt cggaaaagaa
     3601 gctctgtcgg cggtaggtgg cggcaccggt acgctgatcc agctgttggt gggatttttc
     3661 gtaggattgt ccagcggtgc cacggtcatt atttcccagt attatggggc aaaacgtgcc
     3721 gagatggtag gctatgccgt acatacctcc attgcgttca gtctggtggc gggagtcggc
     3781 atgatgatcg tgggtattac ggcggcacca tgggcactgc gtgcgatgtc tacacctgag
     3841 gatattttgg gaccggcgac aacctatatc cgaatctatt tcctgggagt catcggaaac
     3901 ctgatctaca atatgggagc cggcatcctg cgggctgtgg gggattccaa gagaccgtta
     3961 tatttcctga tcgccagctg cctgacgaat attgtgctgg atattgcatt tgtcatcgga
     4021 ctgcatatgg gtgtggctgg agccgcactg gcgaccattc tgtcgcaggc actcagtgcg
     4081 gtactggtat tgtgggtgct gatgcggacg aaggatatgc atcgtctgga actgagaaag
     4141 atccgttttg atggaagaat gttccgcagg atcatccgga tcggacttcc ggcgggattg
     4201 cagtcggtga tgtatacatc gtccaatatt ctgatccagt ccagcgttaa cgaactggga
     4261 acggatacgg tagcggcatg gacagcctac agcaagattg acagcctgtt ttggatgatc
     4321 gtcaatgcct ttggcatatc gatcactacc tttgtggggc agaattatgg tgcggggaaa
     4381 atggatcgtg tacacaaggg agtccgtacc tgtatgggga ttacagccgg ggcaaccgta
     4441 ctgttgtccg tgatcctcta taattatgca tcgatctgtt atcaactgtt taccacggat
     4501 gcacaggtgg tagtgatcgg agaacagatt ctgcattttt tggtacctac atattttacc
     4561 tatattgcca ttgagatttt gtccggtact ctgcgcggta tcggcgacag ctggcttccc
     4621 atgatcatct gctgcctggg gatttgcgca ctgcgcgtgg tatggatcct gacagccgta
     4681 cctctgaaaa atgacattct caccattgta ttcagctatc cgctgacctg gacggtgacc
     4741 agtatcgctt ttattgtgta ttacctgttt ttcagccgcc tgaagatcgt gggacgtaag
     4801 aggtgatttg taagatattg cactttattc tataaaattt gcatagaaac gggatgaaaa
     4861 taaatttata ataatgtgta actattcaga gccatgcaaa actgtgttct gcgaatgctt
     4921 gcattcaggc agataagcag ttttatatgg cgaagtaacc tcatgagcaa aaaggaagcc
     4981 tcgcagaggc ggttttgcga atggggtctg aatagttaca ataatgtcta taatgttctt
     5041 tcggaaaaag gaggtattta aatgtatcag gcagatgaaa aaagatatga agaaatgaaa
     5101 tacaatcgct gtggtgccag tggactactg cttccggcag tctctctggg attatggcac
     5161 aatttcggct ccaatgggaa ttttgacaac atgcaggaca tgtgccacac agcatttgac
     5221 catggcatta cacattttga tctggcaaa
//
LOCUS       DAWEJW010000146         5228 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4683_length_5228_cov_49.291514, whole genome shotgun sequence.
ACCESSION   DAWEJW010000146 DAWEJW010000000
VERSION     DAWEJW010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4683_length_5228_cov_49.291514"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2893..2992
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1459 a         1222 c         1090 g         1357 t
ORIGIN      
        1 ttaagatcgg aatgtgatct ctccggtctt gtcatccatg ctttccacaa tagcgagcga
       61 ctctacacgg atacccttgg aacggatcag gtcgccgccc ttctggaatc ccttctcaac
      121 ggcaatgccg acaccctcta cagaagctcc cgctttattg acaatgtcaa tcagaccctc
      181 cagtgcgcat ccgttggcca gaaaatcatc aatgatcaga atatgatctt cgggatgcag
      241 gaattttttg gaaacgatca cgtcatatac tctcttgtgc gtaaaggatt cgatcttggt
      301 ggaatatact tcaccgtcaa tatttacgga ctgggacttt tttgcaaata ccacaggcac
      361 atgaaaatgc tgggctgcca cacaggcgat accgataccg gaagcctcca cagtcagaat
      421 cttcgtaatc ttacggtctg caaacagatg cgcccactct tttcccattt cattgaacag
      481 atcaatgtcc atctgatgat tcagaaaaga atctactttt aatacattcc ctgcttttac
      541 cgtaccgtct ttgcgaatgc gctcttcgag taatttcatt gtatgtctcc tcttttgttt
      601 cttcttaata tattttaatg atgtcggggt caaaagcccc cgactttgat acctacgaat
      661 tgcttcgtgc tacgcactac cacaattcta cacaatattc gcatatcgtg gaattatcat
      721 ccctctttta tgctcttatg tctcttatta tacgcaggat attgctcctt tttcaataga
      781 agaactatat ttcttgctat tttgacaaca ttttgctatg ttaaccatga gaatactgaa
      841 atcacgaggt gccttatgcc tatcagagga tatatcatag aaaaatataa tactatgact
      901 aacgcttata cctgcaaccg tctggtacag gaagcttctg ccctggatat ggatctgcag
      961 attgtaggga tccatgatac tgcggtctct ccgcatggtg ttataaatca tggtaaaata
     1021 ctgaaacccg tggattttgt catcaaccgc tataaatggg gacgggaaaa ggatgctata
     1081 aatgcacttg ccacccgaag ctataatcca ttaactgctt ataatatcta tattaataaa
     1141 tttgaacagg tacgaaggct tcattccaaa gcctttttga tcccgaaata tcttctggga
     1201 acttcacggt tgcctttttc ttctattgta gaacaactgg gccttccctt tgtcggtaaa
     1261 ggactggaaa gctccatggg agaagaaatc gtctgcatcc gggataaagc ttcttatgag
     1321 gaactctctc ttaactaccc ttcctccaag gaatggctct tcgaagaatt tatctcagaa
     1381 agctacggca gagatctgcg cttttacagc atccgcggag aagccgttgc ctgtatgcag
     1441 cgcacatccc agggggattt ccgtgcaaat gtagctctgg gcgcttcggt agagccttac
     1501 cctgtcactt ccggcatccg taccatagcc gcagatattt acgaacagac cggactggat
     1561 tttctgggaa ttgatctgct ctttggcaga gaaaaacctt atttttgcga aatcaatgtc
     1621 atgccgggac tggaaggtat tgaaaaggct tccggcataa atgttgcggg aaaaattatt
     1681 gagacgatac aatctgactt tacatcaaag acttagtgta aaaaatagtt ttttcatgcg
     1741 caaatatgtg cctgcacagt gtacgaaaat acatgatact aaagtatctg gaaaattgca
     1801 ggctacaaaa aggtatttat atgaataaca cttccgaaac atcactgcaa tttcataaaa
     1861 tcactacgcg taaggaggcg gaagactttg tctatgcctc ttatctgcga gctgcctcac
     1921 atcaggatta tgctgcaaag gacgccagaa aacgacaccc ggaactgacc agatccctgc
     1981 tttggcaaaa atccgttact ccctgcatcg ttgtcaccgg aagcaaggga aaaggctcca
     2041 tttccaacat gatctcccga atcctgcaga caagattctc tgtgggactg atgaccagcc
     2101 ctcatatcac ggactttcgc gaacgcttcc gtgtcaataa tacgatgatc agtgaatctg
     2161 acttctgccg tctgatgaca gagatccagc cagagattct gaatattgcc tctgatctgc
     2221 cggagtcagt atgtatcagt cccatgggaa tccaggccga tctggcactg gcttattttt
     2281 cggaaaaaca tacggatttc aatgtactgg aatgcggtaa aggtgcaaaa tacgatgatg
     2341 tgaataatgt acgtcacaat tatgccgtga tcaaccggat ttttctggag catacgaggg
     2401 aactgggtga tacgctgact gccattgcgg aagataaatc ccacgttatc accggagagc
     2461 agaagtgtgt ctattttgcg gagcaggaac cggaagttct ggaagttctg cagcgccgtg
     2521 ccgaaactct gcatattccc tataagatct acggtcggga ttttcatgcg gaaaacatcc
     2581 gttatacctg cttcggaatg cagtttgatg ttgaaatcgg agacaacatt tatgcggatt
     2641 tacaaattcc gttgctggga gaacatcagg cgaaaaactg tgctctggca cttgctgtgt
     2701 gtgtggatgt gctgtctgat ctgcgcaggg aatccgcagt tttttctttg ccagatatcc
     2761 ggaaaaatct gtctctgcta cactggccgg gacgcatgga agtcctgcgt tccaaaccgt
     2821 ttgttctgct ggatgcctgt atcaatgcca ccagctgtga gaatgtaaag gaagtcctgc
     2881 gacatctggg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaaacatt
     3001 acgcagaaag gagaagtccc atgacaggta tttacggaat caattattct aataattata
     3061 gtctgaccgg caattacgga tacggttatt ctaccagtgc ggtatcttcc gcacagcccg
     3121 aagagaaggg tatggtggta aatcccggaa aatccgatgt agaaaagccc ggcagaaagt
     3181 cttctcctgc agaatgcgaa acctgcaaga accggaaata tcaggatggt tccaacgaaa
     3241 tggtctcctt caaatctgca tctcatattt caccacaggc ttcctcctcc agagttcgtg
     3301 cccacgaaca ggagcatgta acgaacgctt acaacaaggc cgcacagaac aacggcaagg
     3361 ttctctccgc cacagtgtcc ctgcgaaccg cggtatgtcc cgaatgcggc accgtctaca
     3421 ccgccggcgg caccaccacg accaagatca gttaccgtga cgagagcaat ccttaccagc
     3481 aaaataagaa atctgccgat gctgccatgc tgattggacg gaatctggat ctgagtgcgt
     3541 aaatgaaaca taatttttga cacgcgaaag cccccacctt ttatgatatg tgagggcttt
     3601 tttattttca ttttaatacg ccacatacca gtcccggcag ggcaggagag tgaatccatg
     3661 gaatttttcc gcatctgtat agtaaatgat atatatctta tttctgtcaa tatgctccca
     3721 gtcgattccc atagtgaagc ggattccctt gcctaggaaa gactccggct tgggaggtgc
     3781 atcgcttaag ttcctgttgg caaggtattc tgcagcgtca atctctccgt agagcaaaac
     3841 actgggatat ttgatggcat cttcgatctc gatctcacga ccgctttccg ccgcaatctc
     3901 catggctttc tgtacggctt cctcgctgcc ttcctgaaaa taggcactga ccagctcttt
     3961 ataagaagta aaatatgcct tttcaaactg tacattttcc cccagcagaa gtgccgccaa
     4021 aagaatgctt actgcaattt ttccatagaa attcactttt ttccccagaa aatctgtcaa
     4081 agaagacacg cacaatgctc cgcacagaac aagaggaata tagatgcagt tgatctgggt
     4141 cattcccacg gtaaccagta atcccagcag caatgcgccg gtcagttgaa agaatatcat
     4201 ccataataat gttgtctgtc tcttctgtaa ggctaggatc aggcggatca ccagtaaaaa
     4261 aactcccaga aagatcagta ctctggctgt gattccgaaa aatccaaacg gcatgatcac
     4321 atcgtaggga cgaccgatat cctgccgata cagaagatac agcagatttt tgaaattgta
     4381 taaaatatca cggaaagagg atgctatttc attcactctg aaatccgtca ttacatagac
     4441 cgaaaaaggg cctatggaga actctgggag gattcctctg tttaccagca aaaacgccag
     4501 caacggaagt gccagtaatc caatgatcac cacatggatc acgaaatatt tgtcgatctt
     4561 caaagtcttt gtaagaaatc cgtatcccca ttctaacaac agaaggagtg gaacaatagg
     4621 ccagatggtc gcataacaat acaggctcag accgtaggaa agtgccgaca gaataagtaa
     4681 tctcggtttt ttcaggccat aaacaaaaaa ggtcagtcca aaaagcagga acccgggtgc
     4741 cagattggac tccagccccc atcttgacat cataatatgc cagggacata cactgagaag
     4801 aagcatggcc cacagtccgg ccgcttcatt caccatctct ttcatcagaa aagaaaccgc
     4861 caccagagat aaaattgcta caatggcctg cggtagtctt actaccagtg gagtgatctt
     4921 acctccggtg aacaccataa agggaagcat gagatacgag ttcagtgcat tctgcccgcc
     4981 tccccaggac tcgaaataca caggatagga gtgtcctgtg gaatcgatcc cactgtgaaa
     5041 cagtgaatac gcattaaaag ccccataagc ctcgtccatc tggtaaccgc ccggaacctt
     5101 ccccagctgc accaaccgca atatagaacc aaatactaat atgagacaaa acaatatggt
     5161 gtatttatgt tgttctattg ttttctttaa tgtcataggt cttttatatc ctttttgctt
     5221 tccaatat
//
LOCUS       DAWEJW010000147         4995 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4897_length_4995_cov_59.668623, whole genome shotgun sequence.
ACCESSION   DAWEJW010000147 DAWEJW010000000
VERSION     DAWEJW010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4897_length_4995_cov_59.668623"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2199..2298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1078 a         1397 c          892 g         1528 t
ORIGIN      
        1 cgatccacca ggctctcaga acgggagata ttatagctgc ctttccagat gtcgccatta
       61 ctggattcca tatagtcacc ggtgttctgg tcatacgctg ccagcgttgc cgcagccgtt
      121 cccttcagtc cttcttcgat ttcctccatc atggaactgc gtaccgtcgt cagcataaaa
      181 aagatagata acagtcctag caatactacc ggtccgatgg tcattgacag aatctttttt
      241 tgtatactca tgaaaatgcc ccctattcta ccacagatac ggtcttgaaa taccagttga
      301 tacctccggt gatcgttgca tcatccagcg ttgttccttc tttgcccaca gtctctcccg
      361 tattagtctc gatcacaccg tcaaataccc cgttttctcc ggataaaatc tgcttcttgg
      421 cttcttctac tttctctttc gttccttccg cacagaaatc cgccagatcc gtgatgctca
      481 ccagattttc attcatgccg ccgtaatagt tgctgccgtc ccaactgccg tcaatgacgc
      541 tctgcactgc tgccgtatag taagcactcc agttccatac cacactgcac aggcaggact
      601 gcggagcatc cttactcata tccgaattat atccgatact atacacaccc ttttcctgtg
      661 ccagtgtctg gggatatacg gtatcacaat gctgtgcaat cacatcacag cccatatcca
      721 gtaacgtctg tgccgcagct ttttctccct cgggatcgta ccagctgttt gtcaccttta
      781 cgtatacctt tgcgtccgga ttcaccgaat agatgccgag tgcaaaggca tcaataccgc
      841 cggtgacctc cgaattggaa gaatccatag ctgccacata cccgatttta ttggaagaag
      901 tattcattcc cgccacgata ccgctcaaat atctcgcctg ataaattctg ccgaaatagt
      961 tgttgaaatt tttaccgttg ctcaagtagc cggtgccatg ggagaagtac acgtccggat
     1021 attcttccgc catttccgct gtagtttcca tatatcccca gctggtagtg aagatgatat
     1081 tacagccttc atcaatacat tcctgaattg cctgtctggt ctcctgcgta tcactgtcat
     1141 tgacattaat cttgcggatg atctgatcat ctgataatcc cagattctgc tgcattccct
     1201 gaattcccag atcatgcgta tatgtataac cgctgccctc cgccggatcc gacagatgca
     1261 atactcctac cttgatctcc tcctttggaa tccctgcagt ctgtgttgtt gcgctgctgc
     1321 cgctttccga ctgcaccacc tgcgttgtcc cattgctacc ccttgcctca tatccgatgt
     1381 ttgtagcata atcgtccact tctccggtat ttccgcagcc tgtcagtaat gcacacccca
     1441 gaagaattgc cgctgccttc caaaagtttc ctgttttacc tcttcccata tacaagtacc
     1501 cctttctctt ttgtagtcta tgtatatctc ttatctctac cgtctgattt ctcagaatcg
     1561 gttgattcta caaatataaa ctacagcctt cccttattcc ctgctgccat ggcaggtaaa
     1621 ataaatcacc tgatactctt tcgcgatctc tgtaaggaac cgcattgctc ccttggtccg
     1681 gttctcatcc agattcacaa aagggtcatc cagaatcaaa aacggcttct gttcctcata
     1741 catggcttcc accaaagcaa gtctcataca gataccgatc aggtcacggt tgcccagact
     1801 caggtatccg atgtctctgg gcatatttcc ttcaatcacc tgtaatctgg tattggcatc
     1861 catctgataa ttttcgcatt ccgttcccgt gagcatcctg taatatttcc ggaacccctg
     1921 catgacaggt tctgtatatt tcgcagtgaa cgaactcttt gcctgttcca acaaattttt
     1981 tgtcattcca agcaattcat attgtttgaa tccttcctca gtcttcttct gcaacgcttt
     2041 tagctcttcc tgcatattct ccacattatc ccaggattcc tgtaagtcgg aaagttgttt
     2101 ctgatagctg tcgatagtct gaaaacattc ttccatacgc tctgaaagga cctgcatttc
     2161 atctgccaga ctttccaggg attcctctcc ctccgggann nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnncc tgcttctgcc gctccaaaag tgcctcgtaa tctcttctct
     2341 tattctgcag ttccacaagc caggaggtca gttccagtcc ttcccgaagt ccccaacgag
     2401 tgcagaaatc atcaatctgt tcttcctcta tgcggacctg tgcctcaagt tcttccattt
     2461 cctgttccaa ataggaaagc tctgtatttt ctcccgtttg gactttggat ctgcctgctg
     2521 taaacaacag tgccgctgcc acaactgcaa ttcccagtac gatcaggact cctccgatca
     2581 ctccctgtcc caacaggatc acaacaaaac cgccaagcga taaaagaatg ccaaaaagca
     2641 atagtatttt tttcctttta cctgcttctt cctgctcccg taagcatctg gcatcttcct
     2701 gtgcctgtat ggattgcagg gttttccgct gtatttcttt cgcctgcaga agactttttc
     2761 cttcattgcg gttcgtccag gcatttttta cactgtcgat ctccgcctcc tgtggcattc
     2821 cttccgcaaa atatccggaa agctcctcta accgtctgct ctcttcccct gacagggcat
     2881 tttttcttat cgtactttcc aggctctcaa gttcttgtgc ctcagcatat ttttcttcca
     2941 gccgggcatt gtccaccatg gggaaattat ctttacactg cgcctgttct tcctctgtca
     3001 ggcaatagat ttccattgcc ttgcggacag aaatcccctg attgcagacg gctgtttttt
     3061 cgcgtaattc ttcccctttt ggtatcacgc cgttaaaata gctttccgct tcttttaaag
     3121 tctgctgtct ctgtgccatt tttgcacgca gatccaggta cttctctttt ttcgcctgtt
     3181 gatccttata aattcccagt tccttttgtc tctcctgcag ttctgtcagt tccgcagaaa
     3241 gcttttctct ctcctcgatc gccgcctttt tccgggtatc aatactttcc atcgtattct
     3301 gtatctcctg cccgttacgg atctgcgtct tcagttccgt aatgcggtcc ttgctctgac
     3361 ggagcgatcc ggtcttacga gagggtgaca tgctgtttaa cagatcgttc aaccgcttgt
     3421 ccgctgtctc aaaattattg atatcatccg tattctccgc cagattcccg attttggcat
     3481 tgatgccgtc cgtaacaaag gtctcgcagt cattctgtga gataaaaatc gttcgttcaa
     3541 aggatcctgc gtcgatccgg aacaactctt ctcccagatt ttgtgtaaaa tcactgcatg
     3601 gaagatttgt tttcgtatct gtcagctgaa attcatcttc tgcgctttta cttccgaagg
     3661 ttctgcggac agaatattgc tttccttcac tttcgaattg cagttctccg ccataaacgc
     3721 ccttttgcca gggactgtat ctcttcctct cattttccag cgggtctctc ttcccttcat
     3781 tccggaatcc gaacagcata acgcgtaaga atgctgccag tgtgcttttt ccccaaccgt
     3841 ttccctcaca gacggtgttg cagccatctg taaaatcata tgtaaagtca cttagttttc
     3901 cgaaattctc aatgtgacat ttgatcagtt tcatgcgtcc aggatctcct ctccggccag
     3961 tgcctgcaat ccgtaatgta tcactgtcgc cttgtcctct tccggcagat ctgccctgcc
     4021 catgacagta cggataaact ctccctttaa ggacttatcc tttgcaaaag cagcataatc
     4081 caccagatac tcagagctgt ccttaatctt cacaaaataa aaaaggtggc tgaactgatg
     4141 taacagaaag tcaatatcct tttcactttc cacatctatt ttcccgacca gttcatactt
     4201 gatcagactt ttctccgggt aacctttccc ggctgtctcg gctctcatcc gttccatgat
     4261 ttccgtcgtg gattcacaac cggtaatatc caccgggatg gtatagagct ttcgggatgc
     4321 gaaaggaata aacgtactgt caattgtacc tgtcagctca tcaatgtcaa gcatcacaaa
     4381 tccatgctct ccgcattcat caaaccctct gccctccaga cagccgggat aacaatagac
     4441 gcctctcgca tccagacgct cttttttata gctgtggatg tgtcccaggg caagataatc
     4501 aattccctta tttttcaatg ccgtcagatg aatgatctcc gccttatctc tcgcactgta
     4561 ttccgattcc tgcccatgca ggactacaat gttgcattga tccacatcca gtgacagcgt
     4621 attatatatg cttccactgt tttccggtgt cagttccacc ccagtaactg caactccctt
     4681 acctgaggta ttctccgtcc ctgaaagttc atagcttgtc cagttgttcc caaatagatg
     4741 aagattctcc ggaagttccg aaagaccgga cgtaaagctt ccttcgtcat gattcccctg
     4801 taagtaataa aaagctattt tgggatgtcc ctgaattaag tcttttacca cattccgggc
     4861 ggtagcggat acgtttttcg tatcgaacag atctcctgcg attatgatcg ccgccacgcc
     4921 ctgctctgcc ccgtaggtaa ccatattctg aaaagtagtc agcaattccg tcttgcgttc
     4981 tctcgctttt tcttt
//
LOCUS       DAWEJW010000148         4906 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4974_length_4906_cov_51.489177, whole genome shotgun sequence.
ACCESSION   DAWEJW010000148 DAWEJW010000000
VERSION     DAWEJW010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4974_length_4906_cov_51.489177"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3511..3610
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1240 a         1294 c          939 g         1333 t
ORIGIN      
        1 gaagacatct ggaaaggaat ggtgatgtcc tgataggcat ccaccatgat gacatcatag
       61 gtggtatcca ccgcctgcaa aaaagccctg ccgtcatagg tagtcacctt cacatcctcc
      121 ggcagggaaa aatattctct cgacagctcc gtgatctttt cgtcgatctc cacgccctcg
      181 atattcatat cgccgaaata tttccggcac tgggtcgcat aggttccggt tcccatacca
      241 aggatcagta catccatgtc ctctaccggt ttatccggca ccataagggg cgccgccatg
      301 gcatagtcat aatacatgcc ggtaagtccc ccttctttca tatagaggga ttggacaccg
      361 aacagcacgt tggtggacag gaccacctgt ctgtccgtct ccgatacctg cagataatta
      421 tagatggact ctccttcgta ggtcagatcc ttctgccaga aagcaaagga atccgaatat
      481 cccagaccac agcataaaat aaagatcagt accgatgctg caatcttttt ctttcccgta
      541 tgctggcaga taaagtaaat cacagacagc aggatcagaa ttccggaaaa gatcagaaat
      601 gtaatggagg ttcccaccgc cgggatcgtc acgaaagtcg gtacgaaggt tccgatgatg
      661 cttcccacgg tattgaatgc acctaaagtt cccacggtct gtccgctgtc atccagacta
      721 tccacggaat attttaccag agaaggggtc acagttccca gtaaaaataa ggggaacacg
      781 aagatcacca tgcaggctgc aaacgccgca atgatgagga aattgttgct gacggtaaag
      841 atcagcagtg cggagatccc aaggataatg tacttgccca ccacagggat cagggcaatc
      901 cagatggccg cgatcaggat cctgccgtag agtttgtccg ggttcgggga cttgtccgca
      961 cttttaccgc cgtagatatt gcccagcgcc atggcgatca tgatggtgcc gatgacaatg
     1021 gtccatacga tctgggaaga actgaaatac ggtgccaaca gtctgctggc tcccagttcc
     1081 accgccatga cggacatccc ggcgaagaac tctgtgagat acaagaacag cttatttttc
     1141 aaaattcctg aatgattcat aagtgttgta atcctttccg cattgacagt gcgtctattt
     1201 tacgttatga ttttatcaaa cggtagtaac taccggcaat acttttttgt aacttttcag
     1261 agccatgcaa aactgcgttc tgcgaatgct tgcattcagt cagataagca gttttatatg
     1321 gcgaagtaac cacatgagca aaaaggaagc cattttatgt attctatgaa agaagcctgt
     1381 gccctgaccg gcatgaccta tgagaactta aaattttact gcaaggaggg actggtaccc
     1441 aacgtgaagc gggacagccg caattatcgt gtcttcgatg accacgatat caaatggatc
     1501 cagagtctcg ggtgcctcag aaactgtggt ctctccatcg ccgaaatgaa agaatatctg
     1561 catttatgtc tggagggcga atcctccatt cccgccagaa aagacatcct ctcccggaaa
     1621 aaagaagcac tgttaacctc catgcagcag ctgcaggagg ccgtggacta catcgactgg
     1681 aaacaaggat tctatgatga cgtgttatcc ggaaagaccg cttattacag caatcttatc
     1741 cctgagacga caccggatgc ttccgaaagc acagcttcct gaaactgttt gcgcaatcta
     1801 aaaagcggcc atgcgattaa agcatggcca ccatgaaaac tcatctctgt tattaccgga
     1861 tcaaaagtaa ccatgccatt ccataatagg ttaccactgc caccagatcg tttaccgtgg
     1921 tgatcagggg accggatgcc actgcagggt ctaccttgat cttatggaaa aacatgggga
     1981 tcaccgtacc tacggcactg gagatcgcca ttgccactaa gagggagact cccacgcagc
     2041 cggagattcc aaaggctccc atccaggtat atcctttgaa cagatgcaca tagacaccga
     2101 gacaaagtaa tgccataatg cccaacagtg ctccgtttaa ggaaccggtc tttacttcct
     2161 tgaccaccag ctggaacttc tgttttcctg tcaggttctc gtccatcagc acacggattg
     2221 tcactgccag ggactgggtt cccacatttc ccgccatatc cagcaccaat gactggaagc
     2281 atatcacgat gggaagcact gctaccacgt tttcaaaaag tcccaccaca gaggataccg
     2341 ccatgcctaa gaacagcagg atgatcagcc agggcagtcg ctttttcatg ctttgaagtg
     2401 tgggctcctt cagatcctct tctgcggtca gacctgctaa ctttgcatag tcctcgccca
     2461 tctcattgtc taccatttcg atcaggtctt cggatgtgat gacaccgagg attttcttat
     2521 cttctgacaa tacgggaatc gagtcctccg cataatccag gatcctgtcc atacaatcat
     2581 cgatcttctc atgatcggtc acataaggat aagatttcaa caccagatct tccagatgcg
     2641 cattttccct tgcccggatc agatccttca gatcgatggc tccggcgaaa acaccctttt
     2701 catctaccac atagatggtc atgatattgt cgttttctcc ggcctgcttt accaactcgc
     2761 tcatagcctg cctgatggtc aggttctccg ggatacagat gtagttcgtc gtcatgcagc
     2821 tgccgatctc ttcttcatca taggacagca gcatcttcac atcttttttc gcatcctcgt
     2881 ccagcatccg gacgatctgt tctttctctt ccggatccag atcttccaac aaatcgacgg
     2941 cgtcatcaga atccatctcg gagatcactc tcgcagcact ctccacagac agttcccgga
     3001 tatacttacg gggttcctca atgtatttga agatctctgc gaccttctgc gctcccagca
     3061 gatgatacaa tcgcttccgt tcttcctctg tcatccccgc catcatatcg gcaatatcgt
     3121 ttttatgata attcaataaa aaatccagga tctcctcatc cgttttctcg ctgcgaaata
     3181 attctgccag ttcggaatat ttcttgttca tagtctaagt ctcctttctc gttctcatct
     3241 tttaaatgca acgtaaaggg caaaacaaaa gaagcatcac acgatgcttc ctctgcataa
     3301 atctctatgc acaacaaatc aaaatacggc cgtcactttc cacagacagc tgtaccctgc
     3361 tcgcaggaaa aaagcaccgt gttttctact tcgcccataa ccatcgcaac tgtccatgaa
     3421 attcacctcc aacttgtttt tttcgtggtt atagtagcac tgtaccattt gttttgcaag
     3481 ctgtttttct tatttttcaa ttgcattttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn gtatgcaaac atagacttgc tatattcgac aaattcctcc aaagacactg
     3661 tcttgtactg atccggcata ctctctttat catcttccgg catgtcatac tctgcagcct
     3721 gctcgctgtc tccctactcc atccgctcta caatgcagtc aaaaatatct cttttactct
     3781 tgtaatggcg gtataatgct cctttcgtca tgtcaagctc tccggcgatc ttttcattcg
     3841 caaaaaagtc atgctatatc atacatctct ctgcagatct ttttcccagt ttccgtccgg
     3901 aaaatattcc gataagcgtt ctgcaaggct tcttcagaca gttcgtcctc acacatgttt
     3961 acaaggaaat ccgcttccac cagaatctga taatcaatgc cgtctatatt gttgtaggta
     4021 tgatgatggg caatcagata ctgtacccgc tcagaaactt ctccggaaaa accaagatct
     4081 gacagcagct tttcagcaat gaccgggcct tctttttcct gtaattttcc gttacagttt
     4141 ccgtattttt cttcacacag atggatgccg atatcatgag tcagtgctgc cgtctccaaa
     4201 ataaataagg tcttagcatc cacattctcc atttccgcaa tcagcttcgc atagctgtgt
     4261 accttgcaaa aatgctgaat gcgctttgca tctccatggt aaagttcgat cattgccata
     4321 tgtaatttgt tgatcatttt attttcctcc taccgggttt cctttgtgat gctttccgtt
     4381 ttcacgggcc tcctgcctcc gaaatatcct actggatctt tgatacaatg ccttcttccg
     4441 tcaatgtctg caaacattcc tgtgcattct catcatgcat catctgccac acctgctcca
     4501 taccgggcgc cagcggcttc ttcaaaaact gttctccgga gatccagtag acctttccct
     4561 cctcggatga gatcaatttt ccttcatatt ccgacgtttt atataaaaat cccacattcc
     4621 ttatatcccc tgtcatccag tgataaatgc cggtcagtct gggattttgg atcttcagtc
     4681 cggtctcctc atagatttct ctgatcaccg attctttgaa agtctcaccc ggctccacat
     4741 gccctccggg aaatgtcgtt cccgaataat tcttccccac cttgtccagc gccaccacat
     4801 taccttcttt atcatagacc atacacatat tcatgaaaat cactttttca attccagcca
     4861 ttactctctc tcatttctct aagataaact taatataaca taatct
//
LOCUS       DAWEJW010000149         4893 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_4992_length_4893_cov_59.543406, whole genome shotgun sequence.
ACCESSION   DAWEJW010000149 DAWEJW010000000
VERSION     DAWEJW010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4992_length_4893_cov_59.543406"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1485 a         1088 c         1087 g         1233 t
ORIGIN      
        1 aggtaaataa agaagctgaa aaacgcaaca tacagagaac taagcgttaa tgccgcttta
       61 gatgtggccg tccgtgtcct tttgtttttc accagccaga ttgcgaatac aaagcccgca
      121 ataatcaaaa gcgcgtacac tgcaaagatg atataaaccc acggcgcatc catgccgatt
      181 ttcaagtgcc ggatgataaa cagggtcagc gggagtacgc ttaccaacaa agataatctt
      241 cctaaatttt ctgctttcat tttttctcct ttatccggct acaacatctg cctttttcag
      301 atgattagta gccaaaatca caaataatac cgtgtaaaca agaaccattg gaaccaaagt
      361 cattatgctg tcaatgggca acacttcgtc ctgtgacgat agaccgagaa taatttgcga
      421 ataagggaac atctcactta aaccagcaat atggaaagcc agaccgccga tactaaggca
      481 aaccgccaaa cctaccggca ccgcaaagct cctgatccgc atggacaagt aaagctgaac
      541 agcaccaacg cttaaagctg aaaaccagcc cataaacagc caccatagca tattgatagg
      601 gatgggctgt tcaaaccgaa acacaacctt tcctacgata atgataaaca ccatcaaaag
      661 aacctgtgcg gtcagaatag attttgtcag cacaaccagc ttcgcaatga aaatggttgg
      721 ccgggaaacc ggcgttgtca taaggctgtt ccagttgtga tttgtgtgtt ccagccgcca
      781 caaataggac gcacaaatgg caatcaggat tggaaagaaa aaatacccat agaacagccc
      841 tgtttgaagc caaagctgct gccatactgc gccgtcgtac aaattggccg aagcattgat
      901 gccataaatg attgcaccaa tggcggcggt caaaagtggc agaatgagaa ctgctaccca
      961 catattagaa tggcggcttt tcagattttc cgcccgaatt gcattgataa gcattacaat
     1021 ccctccttta atactcgtat ttttgaaact tcttctcaaa caggacaaag gcaatcaatc
     1081 caacaataaa gaggatcgta agcggaagaa agtcaatatt ccgaacgaca agttcactgg
     1141 attgcaaatt gccggaagaa gtgtcctgcc ccagcaccat catttctgcg taatttcccc
     1201 aaatgaggat attgcggatg cctgtgggga gcaaagtaga aataaagcca atcaacgcgc
     1261 caatcatacc aatagacatc gggacaagct ggttcgcaaa aacaagagag aaaaacacct
     1321 gaatagttac aaccacaaat gtaaccatta ctgtccccaa aacaaattcc cacagcgttt
     1381 ttaccggcag cggctcaaca atccccacag cattaccgta ggcgacaatc acaagagcct
     1441 gaatgagaat tgccgccagc atgagaacgg ctactacggt aaacttgcag aaccaaattg
     1501 ccttgcggtt ttcattgttg cactccaaca acttccatgt atttccccgg tgttccatat
     1561 cgcaaatgcg gctggcaata accgctgaca gaatgggaag aaaaagccca ttcatagtag
     1621 ctggcccaac gatcaaatcc tcccatgccg gtgcattggg gtcggaaatc ataccgagga
     1681 aattttcatt gtttccatag ttggaaaata caaagagcaa ttccacaccg aggatcagca
     1741 caaaggtcag aaaaaccttt ctgcgtttca gtttgtataa ttcaaggcac aggtatttca
     1801 tcacagtgtc agctcctttc ctgtcaggtt gaggaaaata tcttccagac tttcccggtg
     1861 ttcctcaaca cgataaacag agataccatt ggaacacagg cattgcacaa cagcgccggt
     1921 ctgttcgtca gtcagcgcac cgatctgcaa accatccgtt gtacgctgcg ggttggcctt
     1981 tttaagaagc tggaaagcgt ggttgttatc gctggttctc aatataatct gtggctcccg
     2041 ctgcatatcc agaactgaca tcctttcctg aaaaatcagg cagccctgat tgataatgcc
     2101 gacaacagta gccatctgat cgatctcact taaaatgtgg ctggaaatca tcactgtcat
     2161 gccatactgt ttcggcagca tcttaatgag ttcccgcatt tcttcgatgc ctgcggggtc
     2221 aagcccgttg gtcggttcat ccaaaatcag aagtttcggg aaccgggcca gtgccattgc
     2281 aatgccgagc cgctgcttca taccgagaga atagtttttg actttcttct ccatctggtt
     2341 ttccatacgg acaatatgca ccgcttcttc aatgttcttt ttgggcaagt caagcagcct
     2401 gcgaatgatc tccatattct cgcggccagt caaatgtcca tagtaggacg gggattcaat
     2461 cagggaacca acggacgcga gaatatcacg gcggttcttt tcgttgaacg gttttcccat
     2521 aatttcaatg gttccgctgg tgggtttgac aagtccgagc agcattttca tggtggtgga
     2581 cttgcctgcc ccattcgggc cgagaaagcc gtaaatgtcg ccctctttaa tccgaatatc
     2641 taattcctga acacggtaaa caccgtcata ttcttttgac aaattatctg ttgctacaat
     2701 cattttttct tgctcctttc ctaagctgct tatatcatat agcccccacc ttgcattgca
     2761 ttggagagaa ccttgatttt accttgattt tcacgatcaa agctgcaaat atgaaaagat
     2821 atggtataat acataaggaa aggagtgagc cgatgggtac aatcaaggac aaaattattt
     2881 tgctggttga tgatgaaccg gcaattttac agatgtgcca atccattctg aaagagaatg
     2941 gttttgaaaa tatagtaacc gccaataccg ccctgagcgc attggaaaga atgagtgcaa
     3001 agccggatat ggctgttttg gatgttatgc taccggatat ggacggcttt atgttggcaa
     3061 agacaatccg agaacagaac ccgatcccta ttcttttttt gacggcaaaa ggagaaatcg
     3121 acgataaatc aaaaggtttt gaggccggtg cggatgatta catggtaaaa ccatttatcg
     3181 cgcaggaatt tgtatggcgc attacggcca ttctgcggcg aacctataaa gaaagtgagg
     3241 aaagcagctt cttactggat gcctgcaaag tggacttaga acaggcagaa gtctatcgtg
     3301 agggacagga accagtttcc ctgaccgcaa aagaagtgga gatattgaaa aaactgtatg
     3361 caaatgcggg ccgcattgta actttgggtg cgctttgcca aacggtttgc ggggatattt
     3421 atataggcta tgagaaaaca cttatgtcgc acattcgcca tatccgggaa aagattgagg
     3481 aaaaaccatc ttcgccggtt tcgctggtaa cggtaaaagg cattggatat aagttgatct
     3541 taaaggacaa gtcaaggtga gcctatgaat aatattcaac gattttttaa gcggtatgtt
     3601 cattccagtg tgaagctgtt ctgcttcttt atcctgataa acattgtcct ttttgttgtg
     3661 ctggctgtgg tgtcaagcag tcggggcagc catagcccaa cgcaactatt agagcaggtt
     3721 tccgcagact tgcagcaaag tgaagaaagc taccgtcttt cagaaaatac ggctatccaa
     3781 atgacaaatg aaaacgcatg gtgtatgctg ataagcaatg acaccggcga tgtggtctgg
     3841 aattacaaaa taccgtccga attacctctg cactatacac cgggcgatat tgcagaaatg
     3901 acaaggtggt acttagagga ttatccagta ttcgtgtcaa atcaatctta tggtctgctg
     3961 gttgtcggct acccgcagga tagtttttgg aaactgtctg cttacaaaac ttctgacagc
     4021 attaaaatag acatatttgg attggttact ttatttttgt tcaatatcat cattgtgctg
     4081 atcctgttca ttcacaataa ccgcaagatt gaaaaatcca ttaagccgat cctcaccggc
     4141 atagaagaaa tttcatctgg gaagaaaaca cagttagttg aacgggggga attggcggaa
     4201 atcaacgcaa agctgaataa ggtcgccaaa accctgcaac gcagggacag ggcaagggca
     4261 aactggatca gcggtatttc acatgacatc cgcacacctc tatcaatggt gcttggctat
     4321 tccagcagct tagaggaaag ccgggatttg aacacagaac agaaagaaaa gattgtggca
     4381 atccgccagc aggctaccaa aattaagcag ctcattgagg atttgaattt gacttcaaaa
     4441 ctggaatatg atatgcagcc attacgaact gagaaaattt ccccagtaga attggcccgg
     4501 caagtggtat gtgattttct ggatagtggg cttgatgaaa gcttctctat tgattttcaa
     4561 tcggatgaag aaagcgagct gcactatatg ctgggtgatg aagcgctctt aaagcgggcg
     4621 attacaaatc ttcttcaaaa tagcatcggc cacaatccaa acggctgtgc aataacaatc
     4681 tcggttgact gtacggatgg acatacagaa attattgtcg cggataatgg cgtgggggta
     4741 tctgctgata agttgcaaga attgaatacc aaaacgcact atcttgaaag cacagatgaa
     4801 aaactaaacc tccgtcatgg acttggtgta ttgttggtac ggcaaattgt agaagcacat
     4861 agtggtacaa tgaaaattga aagtgcgctg aac
//
LOCUS       DAWEJW010000150         4862 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5021_length_4862_cov_50.710214, whole genome shotgun sequence.
ACCESSION   DAWEJW010000150 DAWEJW010000000
VERSION     DAWEJW010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5021_length_4862_cov_50.710214"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1440 a          999 c         1311 g         1112 t
ORIGIN      
        1 cattcaccag agactttaaa ccggaatcca atgagaaatt gtcggagaac ggtgttctgg
       61 cagacaagac catgaatacc ctgcttcatg tttttgaagc ttatacggag ctgtaccggg
      121 tatctgggga tgaaaaagtg cgcagacgcc ttctgtggat catgaatgtg ttcgctgaga
      181 aagtttataa tccgaagctc cacagacagg aagtattttt cgataagcat tataatacca
      241 ttctgaatct gcattcctac ggccacgaca tcgagacggc atggctgatc gacagaggct
      301 gcaaggttct ggatgatccg gaactgacgc agaagatgta tgccatcacc agagacctta
      361 ccgcacagat ctataaggtg gcattcgacg gacactctct ggccaatgaa tgtgacagag
      421 gtgtcgtgaa tgtacacaga gtctggtggg tacaggcgga gaccgtgatc ggtttcttaa
      481 acggctatga gaaagagccg gagaaaacgg aatatctgga agccgcagag aaggaatggg
      541 catttatcag agatcatgtg atcgataaga gacccggttc cgaatggttc tgggaagtgg
      601 atccggaagg aaatccctat cctgaccgcc ccattgtgga gccttggaaa tgcccttacc
      661 ataacggcag aatgtgcatg gaggttatga agagatgtta gagcgttatt acatagaaaa
      721 agagaagcag gagaagctgc tttcccggaa aaatgtaaag tctgatttct acaacggtat
      781 ttatgaccgt tatgaatatc cggttctgac cagagaacat attcccctga cctggagata
      841 tgatctcaat cccaagacca acccctattt catggagcgc ctgggaatca atgcggtcat
      901 gaattccggt gcgattgaat taaacggtaa gtattatctg gtagcccgta tcgaaggcaa
      961 cgacagaaag tctttcttcg gtgtggcaga gagtgacaac ggtgtcgatg gtttccgttt
     1021 ctgggactat cccatcctgt tggacgatac ttgtccggaa gagaccaatg tgtacgacat
     1081 gcgtctcacc aagcatgaag atggttacat ctacggtgtg ttctgttccg agagcaagga
     1141 tacctccgta aatgatctgt ccgcagcagt agcagctgcc ggtattgtaa gaaccaaaga
     1201 cttaaagcac tgggagagac tggagaattt aaagaccctt cgttctcccc agcagagaaa
     1261 tgtagtgctg catccggaat tcgtggatgg caaatatgct ttctacacca gaccgatgga
     1321 tgacttcatc gagaccggaa gcggcggagg aatcggtttc ggactgtgcg acgacattac
     1381 ccatgcagtc atcgacgaag agcgcatgac cagcctgcgg aaatatcaca ccatcaccga
     1441 ggccaaaaac ggtgcgggag ctacccccat caagacagac agaggttgga tacatatcgc
     1501 acatggagtt cgtaataccg cagccggttt acgttacgtc atttatgctt ttgccacgga
     1561 cttaaaagac ccggcaaaag tgatcgcaga gccttcgggt ctgctgatcg gtcccagagg
     1621 ggaagagaga gtgggagata tttcaaacgt agtatttacc aatggcgcaa tcgcaaatgg
     1681 gaagggggaa gtctttatct actatgcatc cagcgatact cgcatgcatg tggcaacgac
     1741 tactatagat aagcttgtgg attatgtatt ccacacccct tccgatcccg gaagaagcgt
     1801 ggagtgtgtg gcacagagat gtgaactcat ccgcagaaat gaagagttct taaaacagga
     1861 agcaagataa ataacgcaga aatgaggtaa ctcgaatgag caaagttaag atgatcagtc
     1921 ctgcagtacc taatgtaccc tggcaggaca aacccgaagg ccataccgga gcaccggtat
     1981 ggagatatag tgagaaccct attattggca gaaatcccgt agaaggagta gccagaatct
     2041 tcaacagtgc ggtaatgccc tatggcgatg aattcattgg cgtgttccgc ggtgaacagg
     2101 tcaacggtat tccctatatt tatttaggac gcagcaagga tgctattcac tgggattttg
     2161 ataagaataa gatccagttc gtagatgaag aaggcaagcc tttcatgccg atttatgcat
     2221 atgatcccag actggtaaag gtagaagata cctactatat catctggtgt caggatttct
     2281 acggcgcagc catcggtatt gcgaaaacca cggatttcaa gacgtttgta agaatcgaga
     2341 atccgttcct tccctttaac cgtaacgccg tactgttccc cagaaaagta cacggcaatt
     2401 tcatgatgtt gtcccgtcct tccgacagcg gacatacacc tttcggagat atctttgtca
     2461 gtgagagccc ggatatggta tactggggca aacacagaca tgtgatggga aagtcttccg
     2521 aatggtggga gtccttgaag atcggtggcg gtgcagctcc cattgagact tccgaaggat
     2581 ggcttctgtt ctatcatggt gtgagcggaa cctgtaacgg ttatgtctat tccatcggtg
     2641 gagcaatact ggatattgac aacccgtcca tcgtaaaata tcgttgtgag aacttcctgc
     2701 tgacaccgga agagtggtat gaggagagag gattcgtacc gaatgtatgc tttccctgcg
     2761 ctaccattca tgattccgag agcggcaaga tcgccattta ctatggcgca gcagacagct
     2821 atgtaggact ggcgtttacg accatcgatg aagtggttga ctatatcaaa gaacacagcg
     2881 tggtcacaac aagcgataca gaagaaggca gaagatagaa tcacgggacc cgcgtaaagc
     2941 gggtcctttt tgcttatgga gacagcgtat gaaggaaaat gaggaattat atgccagaat
     3001 cgaagaactg gaaaaagaac tggcagaaga acgtatcagg aggaagatta ccgaggacaa
     3061 ttccgactgt gcgctgtggg agtatgaaat tgcttcaaaa aaatatgtgc tttccagaaa
     3121 gcttggagga aagtggaata ccaccaacat ggtcatcgaa aactatcagg aacagatgca
     3181 taactgggga tttgtacatc cggatgactg ggcgactttt gatgaattct gcgccgcaat
     3241 ggaccggggc gacgagcata tcagctatga ggtacgtcag gtcagtgatg agtctgcctt
     3301 tatctggttc cgttatgtag ggattccggt ctatgataag gatcataagc catataagat
     3361 catggggaag acgcttgata ttacaggaga aaaaaagaac caggagctgt tggaacagaa
     3421 ggcagagagg gactccctga cagatctgta caataaagcg aaaatgcgta agctggtgga
     3481 aaagcatatc cggcaggccg atgacacaga aggagctttt atcatcattg atattgatga
     3541 ctttaaaacg atcaatgata cttgcggaca tttatacggt gatgaagtat tgatcaaaat
     3601 tgccaatacg atcttgatca gtgcaggcat ggaggattat gcgggccgca tcggtggaga
     3661 cgagttttgt gtattctgcc gtgggaaaaa tgtgggacag cacgctgtgg agacagcaga
     3721 gagaatatcc cgtatggcgg atcagatcca gctgtcaggc agacgagtga ctttaagtat
     3781 gggaatctcc cgatatccgg aggatgcttc ctcttatgag gaactgtatc aaaaagcgga
     3841 tcaggcgctg taccgggtaa aacgaagcgg caaaaatgca ttcagcgtgt acgacaagaa
     3901 ctataattat gtcgattata cctcagaacg gaaaggaaca gaccgggaga ccggtttctg
     3961 gcaggagctg gagaaatatt ataaaggacg tcagtggaca gaaacggaaa gtgaaattct
     4021 gtctactatg acgcggatga tggaaagcta cctgaaccat gaagaatatg cagggaatgt
     4081 cagggagcgg gaaaaggata cagaaaagcc ggaaccggac attgccggaa tggagacaac
     4141 ggaaagcttt tcgataaaac tggaacagat cctgccctgg caggacggtc agtatgcaga
     4201 ggtatttctg ggcatccggg attacgatga gatttccgcg aagatgagtg aggaagtgaa
     4261 aaacagcctt ctgtaccatc tggggacgca cattcaaaag cacctgaagg gacaggaaat
     4321 ggtgtgccat gtgaaaaagg atgagttcct attgctgttg gacaatcatg tggaagctgc
     4381 ttcggagaag atcggtcata ttatgatcat gttcgaagct atgatcggag ataaataccc
     4441 cgatgtatcg atcaacctgt acagcggcat ttattatctg gagtccagag gataccgccc
     4501 ggaggaaatt ttccgcatcg gcaagactct gaaaaacaag gcgaaacgtt attgcactct
     4561 ggaaaacagc atctatgaga accgcaagag tgcggggaac atcattgata aagaagccgg
     4621 aatcctgaac cggggcaacc tgaccagact gaaaggtttt atgaaccgtg cagccaaagg
     4681 agagcggctt acggtaggat ttatcggcgg ttccattacc cagggatttt ccgctacgga
     4741 tccggaaaaa tgttatgcgg caaggacatt tggctggctg aaaaaggtat ttcccaatac
     4801 ggaatttgat tatgtgaacg ccggaatcgg agctaccgat tcccagttcg gcgcggcgag
     4861 ag
//
LOCUS       DAWEJW010000151         4808 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5076_length_4808_cov_61.709657, whole genome shotgun sequence.
ACCESSION   DAWEJW010000151 DAWEJW010000000
VERSION     DAWEJW010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5076_length_4808_cov_61.709657"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1107 a         1259 c          989 g         1453 t
ORIGIN      
        1 tagaactgat aatagatccc tttttgttcc atgagctggt cgtgattgcc ttcctccaca
       61 atgccgtcat ccgtcagcac caggatcctg tcggaattgc ggatactgga aagtctgtgg
      121 gcgatagtca gagtagtacg cccctccgac aattctccca gagattttgc cacggcgaac
      181 tcactctcat tgtccagtgc ggaagtggcc tcatccagaa tgatgatggg cggatttttc
      241 agaaatactc tggcaatact gatccgctgt ttctgtccgc cggacagctt tacaccccgt
      301 tctcccacat aagtatcata accgtccttc agtcccatga tgaattcatg agccccggca
      361 cgctttgccg cattgatcac atcttctttc gaagccccgg gacgtccata agcgatattt
      421 tcatagatcg taccggagaa aagatacaca tcctgctgta cgataccgat attgccccgc
      481 agactcttta acgtaaaatg cttaatgtcc tggccgtcca acaggatcct gccttctgag
      541 acatcataaa atctcgggat cagattgcac agcgttgtct taccaccacc ggaaggaccg
      601 acgatggcca ccttttctcc cgcgtggatc tgcaggttca gatcagtgaa taccttgtta
      661 tggtcatccg gatattcaaa gcttactttt tcaaaagaga tttctccctt cgggtccttc
      721 agttctatgg catctggctc atcgaagatc tcgatatccg catccacgat ctgtaaaaag
      781 cgctcgatac cggtcatgcc ccgctggaac tgttcggcaa attcgatgat acgccggatt
      841 gttgcgatca gggtggatac atacagcata tatgccacca ggtcaccagc ggcgatccgt
      901 ccgtttacca taaaaatacc gcctgccacc aataccacca gatacatcaa tccgtcaaac
      961 agcttcactg tggtattgaa tgccgccatg taacggtagg tcagcttctt aatatccaga
     1021 aaagtgccgt tatctttctc aaacttgaca ttttccacct cttcattggt aaatgccttg
     1081 accaccttat ggcccagcag actatcctcg atccgggcgt tcagctctcc gatctggtta
     1141 cgctgcttgc gaaaggcact tcgcatccgg aaattcagga tcatgcacac accgcacatc
     1201 agcggcacgc acagaaagat catcaatgtc agcggcagat tgatatttgc cagaatgata
     1261 aaagagatta cggtcttgat tccggcgatg aaaaattctt ctgggcaatg atgggcgaat
     1321 tccgtcacat caaacagatc attggtgatc cgccccatga tctgaccgac tttggtattg
     1381 ttataataag tattggacag cttctgcaaa tggttgtatg catctctgcg catgtccgtc
     1441 tcgatcttcg cgcccatgac gtgtcccata tcggacatat agtaactggc gatgcaatcc
     1501 acgatacgaa gtccgaaata cagaactccc agtgaaagta tggtcttcac cgacagggac
     1561 gcaagatccc gcagcccttc attggtaatg tagcgcataa ttaacggcaa caccagttca
     1621 cagaccgtgg tcagtgctgc acagaacaga tccatccaca aggtaaaacg gtatttttta
     1681 aaataaggtg caaacctgcg aaacaattcg cgggtggaat attgcttgtt atccattgtt
     1741 gtgttccttt attttggttt cagagctata ggtattgtcc ttctcccaca tagaagcgta
     1801 cttttccttt ttgctcattt ttctggtcat aaagtgtttt gtccgcagcc gacaggaaat
     1861 cccacactcg gtttggttcc ttcggaatag cacagacacc acccacggaa atcgtaagcc
     1921 agggttccac agaagagtct tcattgggaa tctcacattc ccggatgccc atacatagct
     1981 gtcttgcatg ttcctctgca tactccctgt cacgtcccgt catgaccacc aggaattcat
     2041 ctccgccgta acgcgccaca tagtcctccg gaaaacattt tttcagagta tccgctaccg
     2101 cgcagattcc ttcatctccc cttccatgtc catagcgatc attcaactgt ttgaaatggt
     2161 caatatccag gaacagaatg cccagttcct tctcattcag aagacagtct tcaaacacat
     2221 cctccagata acgattcagg cttctcttat tatagagtcc tgtcagctcg tcatggtctg
     2281 cctgatacaa cagccggttt ctcttacgtt cctgttcctt ctgctcctct tctgccgcct
     2341 gtaatcgctt tctaagctcc atcatataca tgacctgact gttttctgtc agctcccagc
     2401 tgtctttcaa gtggaagaag gtgtctgcgc tctgtttgaa ttcttccgga gtcattctgg
     2461 aactgcaata acgcatccgc agagacaaaa gctgcaatag cagactcttg ttctgttcaa
     2521 tggcagcctt tggttccaga cagttcaata cttcctctat ctgttccggt ttgcctatct
     2581 tttccacata ttgcagcaga ttctgaatac tgtcgtattc ggaagagacc tgtgtcatat
     2641 cctctaatgc cattaccgct gtacgtacat gctcttctgc cttcttcctg tctccgtttc
     2701 gctccgccag ataagccaga aatacattga cacccagcct tgtctcacgt atgacctcct
     2761 tgctctgccc ggtctcttcc agtttacaca gtaccttttc cgcctcgtca tccatatgca
     2821 gatgcagcag gcagcatccg tactctgcca gcattttccg gaaattaatc ctgctgtatg
     2881 tactgttgtc atccgcctgt tcaaattcgc ggatacattc cgcatggcac tgcacagctc
     2941 tttcataagc tcctatgaga taatacgcat ccgacatatt gctcagtaca atgctgtgca
     3001 ccatcttatc atcgtatttt tccgtataat ccaatgcttt atcgtattgt tccatggcca
     3061 gcgacaggtt gttctgcata tgtgccacaa taccgatcat attgtacact ctggctacat
     3121 gatgctcctg atccgttccc agaagacacc tgacgctctc atccagataa tgcaggcagc
     3181 cctcctgatt ccctgttgtc agttgataaa aggcagcaaa atagcagatc catccgtgga
     3241 ttcccttatc atcctgttct gtcacagagg taagtgcctg ttccagagtc tcccaggtaa
     3301 aaggaattcc gtctgtcatc tgctgattca ggtgttctgc tgtctctaac atctgctgtc
     3361 tcgttgcgct catatctata tccataccgt cctgttattc tctgccagtt ccgcaaattg
     3421 cggttcgccg gttcctatgc ccagcccgtt gggcataatc tatattcact tattattgta
     3481 ttattttata tcgcagtacg caagtttttt tcctgaataa aatgtttccc aatctcccgc
     3541 aaaaatgtta aaatggagtt atatttcgta agctagtaca aaaaggcatg gttatttcta
     3601 tggaaaatat aacagttaat aaagagtttc atgaactgca ttttgacagc atccaggctt
     3661 tacgcggtct ggctgctctt tttgtggtat ttcagcatgt ccgttttctg aacttcggag
     3721 ctttcggtgt ggacatcttt ttttgtatca gcggattcat gatcatgttt accacggaaa
     3781 aaagtaccaa atatttcttc cgcaaacgcc tggtccggat tcttccgctc tactacctga
     3841 tgacactggg aacctatttg ttgctgttgt tgtttccttc catgttccaa cagaccagac
     3901 acgatttttc ctatctggtc aaaagtctgc tgtttattcc ttttgatatc ggcagcggag
     3961 cgatccagcc tttggtacgg atcggctgga ctatcaactg tgaaatgctg ttctatctgc
     4021 tgtttttcgt tgcttttcac atcagcatga aataccgcgg tctgatctgc agcgcatttc
     4081 tggtgattct ggtgggaatg gtacagatat ttgctccggc gatctcgtcc tggcattctc
     4141 cgataggtaa tgtactttct cctttcctca ttttttacgg agatcctgtc atgttggaat
     4201 ttctcttcgg aattctggct tactatctgc tgcggtattt gtatcgcagg catacccttc
     4261 atccggtttc gcggacagta agcctcgtat ccgcggtatg tatcccggta atcttttgtc
     4321 tgcttgctta ctttacgccc cggatcaatg tgattgggtt cctcaggctt ccccagtggg
     4381 ggctccctgc tctgctattg ttaatccttg cttttctcgc aggcctgcgg ctttccatgc
     4441 cccgcttttt tgtgcagttg ggaaatatca gctattccct gtatctggta cactactatc
     4501 cggttatgtt tctggaccgt gtggtatttg acttttccac cctatccgta ttatctgttg
     4561 tcggactgat cgtaagcgtt gtaataagca ttgttctggc ttatctgtgc tggatcctga
     4621 tagagcggaa attcaccgga tggctgcgta gaatactgct gcgttaattg tatatgcaat
     4681 ttacaaaaaa gatgcacact ttttttattg acatttccgg agccctatag tatattataa
     4741 cttgtccgta agatatgcgc gagtggctca gcggtggagc acctccttgc caaggagggg
     4801 gtcgcggg
//
LOCUS       DAWEJW010000152         4707 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5185_length_4707_cov_63.375967, whole genome shotgun sequence.
ACCESSION   DAWEJW010000152 DAWEJW010000000
VERSION     DAWEJW010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5185_length_4707_cov_63.375967"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2342..2441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1348 a          901 c         1118 g         1240 t
ORIGIN      
        1 aaaacggggc aaaatattac aaatcatatt gaaaaaacgg ggcaaaagag atactgttaa
       61 aaagggagga gggaatttat gtatagaaaa gactctatta tcatagaaga atggttgaaa
      121 agatcggata aagcattatt ggtaacaggc gccagacaga ttggaaagac ctggctgata
      181 agagaggaaa ttgccaaaag cggatatcgt aagtttgaag ttaattttat agatcagccg
      241 gatttggtgg attatctcaa tgtaaaaatg agtgcgaatg agtttctggt gaaattaaaa
      301 atgattatgc cggaggattg taagccgcag gaaacggtcg tcttttttga tgagatacag
      361 aaatgtccgg aaatagtgac aaaaataaaa ttccttgtag aagaaggcag cttcaaatat
      421 gtaatgagcg gatccttatt aggagtagag cttaagggaa taacgtcggt tccggttgga
      481 tatttaactg ttttgagaat gtatccgatg gattttgaag aattcatgat agcaaataat
      541 gtgtcaaaaa caactttgga aatgttgaaa gcgaaatttg aaacatgtca gcccgtggat
      601 gaattcatac atcagaagct attgtcgctg ttttttatct atctaattgt cggaggcatg
      661 ccggatgcgg taaaaatcta tattgctaca aaagatatcc gggaagtgga taaggtacaa
      721 cgggatatcg ttgcgctata taaagaggat ttctctcaat atgagagtga agataaaaaa
      781 ctgaaattga tatctattta tgatatcatt ccggcagaat tgaacaaaca gaataaaaaa
      841 tttgtattta caatgctgaa taaagagtta aagtttgaca gatatgaaaa cagcttttta
      901 tggctgaaag atgccggagt tgcgttaccg gtgtataacg tggaagctcc tgttattcct
      961 ctcaaagcga gcaagagcag taatgtgttc cgactttttt ccaatgacac cggtttgctt
     1021 accagtgcat atccggcgga aaccaaactg gaactgatca ataaaaacag cgaagtaaat
     1081 aacggtgctc actttgaaaa tgctgtggca cagcaattaa cggccaatgg tttggaacct
     1141 tatttctgta aaaagaaaaa tataggggaa ctggatgttt tggtcgaaat ggatgggaaa
     1201 gtggttccca ttgaggtgaa atccgggaaa gcttacaagg cacacaaatc cttggataac
     1261 tttatgaaaa tttctgatta tcatattgag aaagcatatg ttttatcggt agcaaatata
     1321 gaacaggagg gatccgtggt atatcttcct atctatatgt gctatctttt aaaagaacgg
     1381 aaaattggaa agttaatcgt ggatctagac atggaagggt tataacactg ggagaagtaa
     1441 taaaacggga tttgtcgaat aaaggagcga aatagaaatg aagattcgca aggcagagaa
     1501 aaaggacatt ccaagaattc ttgcgctgct ggggcaggta ttacagatac atgcagagat
     1561 ccgtccggat atttttatac cgggtactac gaaatacacc ccctgcgagc tggcagagct
     1621 attggaacag gaggatcacc ctatctatgt tgccgtagac gaggacgatc tgtgcatggg
     1681 ctatgcattc tgccaactca gggagcagcc cttttccact aatatggtgc ccttcaaatc
     1741 attgtttatc gatgaccttt gcgttgacaa gcagacacgg gggcagcata tcggggaaag
     1801 cctgtttgaa tatgtgaaac agcaggcaaa agaactgggc tgctatgaag taaccctgaa
     1861 cgtctgggca ggcaacactt cggcggagca cttttatgaa aagatgggaa tgaagacaaa
     1921 agaacggcag atggaatata tcctgtgagc cggacggagg tggcaggcat gattaaaatt
     1981 gcattttttg atgtcgatgg gacacttctg aagatggggc ataaagaatt aagtgagcgg
     2041 acgctgcatg cattaatttc tcttcgtcag aacggggtga agctgtgtat ggcgaccgga
     2101 agaggatatc tgtcggcacc gcgttttccg ggaattgatt tcgatgctct tctgaccttt
     2161 aacggttctt atgtaacagt aggagacgag attattttta agtgcccgct tgattggatg
     2221 gacaagcttc agatccttca gaatctgaaa ggcatgcata gggcagtagc gataagtaac
     2281 gaacatttta tcatcaccaa cggtacagat ccggatctgg agcagtattt cagattcggt
     2341 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgtcagga agccgaaagc
     2461 aataccgtca gctccgtgct ctaaaagtat gcgggcatct aagagcatgg tctcgtattc
     2521 atcctgggaa tagcagaagc cgcctcctct tgggcggacc attgttacaa tagggatgct
     2581 gcactgctct tttgcacaga tcagattggc ggtagaaggt gtgagaccac ccagatacag
     2641 tgcactgtta agctccaccc tgtcggcacc attttgccag gcggttttca catcttcgaa
     2701 gctgccgcag cagatttcca ataatccttt caaaataatc ccctcaatca atacatgata
     2761 ttattgcgta tgactcttgt ttgctgcttc attgatcagc aacatgattg tgttgatcgc
     2821 atccatttga tggctgcggc tcatggcttc tacataattc tggcggttga agtacagttc
     2881 atgtacctta tccaccagca gattcgtcgt aatatcatcc tcattcagca caatggaata
     2941 accctgcgct tcgaaggaag cggcattcaa cagctggtct ccgcggctgc tggcggcagg
     3001 cagggggatc agaatgttgg gctttttcag cgccagaagt tcgcagatgg cattggctcc
     3061 ggcacgggaa atcaccacgt ctgccatggc gaaaatatcc tttagttccg ccttgatata
     3121 ttcaaactgt ttatatccgg gtgtatttaa caacaggttg tcgattttgt ccttaccgca
     3181 gagatgtact acctggaaat cctccagaag cttcggcaat gcatcgcgga cagccttgtt
     3241 cacgttagct gcacccagac ttccgccaat gaccatgatg acaggtttat tggcagtaaa
     3301 gccgcacatg ttcagtccgg ccagccggct gccctgtgcc agctccgcac gaatgggaga
     3361 accggtcagg acagctttgt cggcagggat atttttcata gtctccggga aattgcagca
     3421 taccttttct gccacgggaa tgcacagctt attggccagt cccggtgtca tatccgattc
     3481 gtggatgata cagggaatat gtaaggaagc tgcagcgcgg acgaccggga cgcttacgaa
     3541 gccccccttg gagaagacta catccggctg gtaatttttc aggtaatttc tcgcttccac
     3601 aaatcccttc atgacacgga acggatcgga gaagttcttc ggatccaggt atctgcggaa
     3661 tttgcccgta gaaatgcctg tgtagggaat gttgaaatcc gcaatcagct ttttctcaat
     3721 gccttcataa gatcccacat aggcaatttc atagccggcc gcctgcagcg ccggtaataa
     3781 agcaatattg ggggttacat gtccggcagt tccgccgccg gtcaatacga tttttttcat
     3841 aataaaatcc ttcttgtcgg tgagattact gtgcgatcat ttcttccagg gcatgagcca
     3901 gctggtcagg acaggaggta ggcttgaaac cgcagcggat ccccttgaga cggctgattg
     3961 cttcttccgg cttcattcct tttaccagag cactgattcc ctgcagatta ccgttgcatc
     4021 caccggtgaa ttttacggaa tcaataacac catcctttaa ttctacatcg atcgaagtgg
     4081 aacaggtacc ttttgtcttg tatatcatat gtaaaaccat ccttttcaaa ctgaatttct
     4141 aaggagtgat tatagcagat tttcggcatt tatacaacaa tatgtacaag gttttctttt
     4201 cacgaaaaat gtaaaagaaa tactttttct gttcggcaac gtagaagaat gacgatgaaa
     4261 aaacgtacaa aacaagcctc taatacgtta tactctaagg aagcgataga aaggagcgca
     4321 ggttatgttt acgattttca aagaagaaaa agtgatcaca accggaattc tggtatttct
     4381 gggacttagt attctggtgc gtctgcttct ggcaggattt taccatacga tgataaaaga
     4441 gacagataat atggcaacga cgaataaccg cctgttgaaa cagtgtaaag taaaatttgc
     4501 aaattgttac cagttaaatg gtggagtatc aaacattccg gtgtttgtgg acaagttcct
     4561 caaccggctg gcgtttggac acctatcctt tgatgcctgg tatcatctgt ccggccagtg
     4621 catgctgttt tccattgtgt ttgccggtat cgggatctgc aaggggatca tgggcggaag
     4681 gatgctgggg gaaatcctgc cttttta
//
LOCUS       DAWEJW010000153         4651 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5256_length_4651_cov_54.432550, whole genome shotgun sequence.
ACCESSION   DAWEJW010000153 DAWEJW010000000
VERSION     DAWEJW010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5256_length_4651_cov_54.432550"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1159 a         1132 c          999 g         1361 t
ORIGIN      
        1 ggaaaacccc tcaggatact tttttacagt gtatgaaagt atctgagggg ttatgcatcc
       61 tgacaattaa gctgctattt gacaattatt ttgtttcaaa atataatagt tgtactttgc
      121 aagtatccat tttgaacata cgggagggtt tttttgtgaa agagtcaaag ctccggctgc
      181 gttccgccgg tttactttct gttgcggcag gatttatgtt gtttctttat gctccattgg
      241 aaatttattt taataacaaa tatgaattct ggtttgattt ttatgatctg atcccgctct
      301 gtctgttgat gtttgcggta tttacgggcg tcagcttcct gatggctttc atcgtttcga
      361 agataaatac caggctatat catggatttc tcacagttta tctgattgcg ttcctctgca
      421 cttatattca gggcaatttc atgataggca atctgcctca cctggatgga agtacggtgg
      481 attgggcaca gtattccgga ttgcgtatcg gctccgttgc tctctgggtg gcagtcacgg
      541 ttctggtgat actgacggtg aaaaagctgt cgcttacaaa ggtggcagat gcagcaggtg
      601 ctgtcagcgg gctgatcagt ctggttctgt tggtgactct tgtaacgcta gggtttacaa
      661 aacagggcct ggaacacaaa ttttctatga tcaacaccac ctatggggaa ctggaaatgt
      721 ctaccgatca aaaccttgta cttctggttc tggacacagt agacggagac atcatgtcac
      781 aggtgatcga acaccatccc gaatataagg aaactctttc tgattttact tattataata
      841 atacgatgtc ggcctatcca ttcaccgcat attcagttcc ttacctcttc tccggcgaat
      901 ggtatgagga tcaggaagaa tttatcgaat atgcaaagag agtctatcgg gaagctcctt
      961 tctttgatga tctggaagcc agaggatacc gcatgggaat gtatgaggag gacgcttacc
     1021 gtctggaaga aagcatgttc cgatttgaaa atatgatcga cacgaccccc acgatctctt
     1081 ccattgcaca atttatgaaa ctggaaatca agctggtcgg attcaaatac atgccttttg
     1141 atctcaagcg cttctgcctc accatcccgg cggaattcaa ttcactggag aagaccgatg
     1201 atatcagtga ttacgaactc ttttcttccg acaatcaggc cttctatacc tacctgaaaa
     1261 agacaccggt aacactgacc gatgacaaat gcttcagatt catccatctg gtaggcgccc
     1321 acagtccctg gcatcaggat gcacagatga acgagattga aaacggcacc tacgaacaaa
     1381 gcattgaagc cagcatgaca atcgtggcta cgtatctgca aaaactgaaa gacagcggtg
     1441 tctatgataa tacggccatt atgatcctgt cggaccacgg atacaacatt gaggatgatg
     1501 acagttccga gaaacggcaa catcctattc tgttcgtaaa gggtgtcggt gagcattatg
     1561 atgaactaca gatttcctct gctcccattt cccaatctga ttatctggaa gcctacacac
     1621 gacttctgga tggcaaacag ggggatgcca tctttgatta caaggaagga gatgccagag
     1681 aacgacgttt cctgttctat gattacgatg agcccactca tttttatgag tatatgcaga
     1741 ccggatatgc cggagatgta gacacgatgt acttcaccgg tgtggaaatc acaccataat
     1801 ggtctcactc cgaaataatc acttgcatta tcttctctaa aaggtataat gtttataaca
     1861 gccttgtttt taacaaatat tgcacgtaca tacgagaagg agggttctta tgaaattatc
     1921 aaaaatcgaa caggtaaacg agtttcttgc tattgttgac agttgtaaag gggaggttac
     1981 tttaagatcc gtagacggtg atatttttaa tctgaagagc aaactttccc aatatattgc
     2041 cattgctgct ttgcttggtc agcatggtga tgaacttgag ttattctgct cagacagctc
     2101 tgatgaggga aaattcatgc atttcctgat ggaaaatccc agtgtacaat agattcactt
     2161 ataaataata tactataata acaaagcact cccattaacc gggagtgctt ttgttattac
     2221 tttaagatgt agtttcacag cagctgcagc aatctccggt tcttatggta gcagaagatc
     2281 cggtcagaca gcatctctcc ttctccgtag acggcattga tatgctgtac ggaggaacgg
     2341 atctcctgta caccggtgca gtctacggga tgcaggattg cttcatgtac gcttggaaag
     2401 actacatagt agtccccgcc cagtttctcc gccaggattg cttttacccc gggatagaaa
     2461 aaggccacag ccccatggat tctggcggta gtggtgagga gatatcctct ctgtccttcc
     2521 ctgtggttcc aggggctgat ccaggtctga tgtccttcct cttccggcag cagaatgcct
     2581 tcctcatagg gaaaaaactt tctcctgtca tctcccagaa acaatctggg cggcatcttg
     2641 cggcaggtgt tcatcatggc ttccgtgatc aactggtctt ccgtaagctt ccatggcagt
     2701 accataccgc gggacacctg tatggtcaga ctgcttccgg tcttctcact ggtcaacaga
     2761 tgcagcacca gcgccacatc tccaatctta cgatagatgg cattgccaag ctcctgcctg
     2821 caccgttcat aattcaccgg acgcacgatc atatgccttc tgcagacagc atagctcaga
     2881 ttctccccgg agagtacgat gtcctcttct tcatcgtcct ccgtcaggcc cgccgagatc
     2941 tccggtaaaa tactctgcca tccttcctgt ccgtatcgct catacaaagg ccgtatcagc
     3001 cagtgctgta tatattctct ttttccacgc atccataatg cgcagagcat atcttcacgt
     3061 atcatgatct gttctcttcc atacatacac cgattcataa tattcatgat atgaaccgcc
     3121 atcggatcac cgcataccat gtgccgttcc agcaggcgta tggtcttgcc ccgtgccgtt
     3181 tccatgttct gcacattcca acgcaattcc ttcttaaatt cttcgtatgt cattgatccc
     3241 ccattatttt ctccctgcaa aagcagagaa ctgatccgta aatttgcgta caaaaataaa
     3301 aggtactatt aaaaaaatcg ttggaaatcc cggccgaacg gcctatttag aattcttatt
     3361 tcagagattt acagtttccg aaaccatccg gatttcggat aactccaccc tatcatagat
     3421 agattgcttt gtctatatgc gatttcacca aggatatctt ttgtcaaatt tcacgaaaga
     3481 ataatttcaa aaagaaaaaa agtccccgtt ttccgcgaaa acagggactt tacaaatgtc
     3541 aaatatgaaa ttacacaacg cgtcgtactg ccaggatcgg acggtaattg gcattgctga
     3601 tcttgatacc agtacgctgt gtgctggcat gcacgatcag cccgccgcca atatacatag
     3661 ccacatgacc gtcataacag atcagatctc ccggctgtgc attgctgtag tccacaccgg
     3721 tgccacaact tctctgctca taggacgtac ggggaatgct gtaaccgaaa tcactgtaga
     3781 tgcggaaggt aaatccggaa caatccgcac cattcgtcag gctggtaccg cccgccacat
     3841 acggattacc tatgtactga caggcatatt ttgcaagacg ctttcccata tcactgccgg
     3901 aggcattttc gatgactgag gtataattgg tcgttgcata agtgccggtg gctgcggcag
     3961 tatttccttg ccgtgcctgc gtctgcagct gttttaactg tttttgttcc tgctgcaaca
     4021 gtgccttcgc tacggaagcc tcctgctttg ccttcttgat ctccgcatca tagttggcgg
     4081 attcctgctt tttcttggtc agcatcacat ccagttccga tttctggttc tccagatccg
     4141 ccttatcggc ttccagctga tccttatcga cctgcagctg ggtcttctct tcttccagct
     4201 ggttccacag cgccagtacc tgttccttgg tttcttcgta ttcctgcagt ttctggcggt
     4261 catactcgta gacgctctcc acgaagtcca tccggttcag cataccactc aggccaccgc
     4321 cctgtaacag aaggttcaca taattctccg aagaatcatt ttcatacatc atgcgcatcc
     4381 gactgaccat atcgtcatgc tgctgttctt cctgcgcctt ggcaatattg tactcttcct
     4441 gggtctgatt gatctggatc tgcttgtcgg caagttccgt ttccttcgct gcgatctcat
     4501 cttccttcat gccgatactg gtcatggtgt tgatgatctc cgcattcagg tcatcgatct
     4561 tctcctgtac cagatcctgt tcatcctgca gggaattgat ctggctgttg atattgttca
     4621 gctggttctg atgttcttta atgttgttct g
//
LOCUS       DAWEJW010000154         4645 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5268_length_4645_cov_64.290850, whole genome shotgun sequence.
ACCESSION   DAWEJW010000154 DAWEJW010000000
VERSION     DAWEJW010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5268_length_4645_cov_64.290850"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          996 a         1330 c          952 g         1367 t
ORIGIN      
        1 gtcacggtgc ggatgctttc cacgatagca ggcagattgg tcttgatctc gctgcgcaca
       61 ccggtgaccc ccatggaact taccacatag ataaaacctt ctgcttccga tgcaatctgg
      121 cggatacggc tctcggaggt aggagcgatc atggagatta ccttcacgcc atgggttttg
      181 gcgggagctt ccacctctgc cttctcttca taaggtacat cgggaataat gatggcgctg
      241 atacccagtt cttcacattt ggtaaaaaat ttctcatatc cgtaatggaa taccggattc
      301 acatacgtca taaatgccag cggaatctgt gactgctccc gtactctctt cacaatttcg
      361 aacacgccgt ccgttgtcat gccgttacca agagagcgga tatttgcttc ctggatcacc
      421 acaccttctg ctgtaggatc ggaaaaggga ataccaatct ctatgagatc cgctcctgct
      481 ttctccatct ccaggatgaa ttgtacagtg ctgtccgcat ccgggtctcc tgcggtaagg
      541 aagccgatga atgctttttt attcggtgta tcaaatactt tttctaattt actcatcgta
      601 tctgtctcca tttcttctat ctcttctatt ctaatttctt ctatctttcc tgttttcatc
      661 ttctgtctgc aggagaacgg actgcccaaa gcatttcaca gcccctgcac gtaggtcggt
      721 catcagtcat gaatatccac accgcggtat ctcgcaatag cggccacatc cttgtcgcct
      781 ctgccggaca gacatatgat aatgctctgg tccgggctgt agttcttcgc gatcttacgt
      841 acctgtgcta ccgcatgggc actctcaata gcgcagatga ttccttcagt tcttgccaga
      901 tattcaaaag catccactgc ctcgtcatcc gtgatgggca catactgtgc tctgccgatg
      961 tcatgcagat aagcatgctc cggtccgatg ccgggataat ccagtcccgc agaaatggaa
     1021 tatacaggag caatctgtcc atattcatcc tggcagaaat aagatttcat tccatggaaa
     1081 attcccagag agccggtagc aatggtagct gcggtcagtg ccgtatccac acccttgcct
     1141 gctgcctcac aaccgatgag ttccacatcc ttgtcctcaa taaagttata gaacgcaccc
     1201 atggcattgc tgccgccgcc tacgcaggct accacggctg ccggaagctt tccttccttc
     1261 ttcagaatct gttcctttgc ttcctggctg atcacactct gaaaatctct tacgatcatt
     1321 gggaagggat gcggtcccat aacggatccc agcacataat gggtatcctc cacacgattg
     1381 gaccattctc tcatggtctc attgacagca tcctttaagg tcatggtacc ggaagttacg
     1441 ggatgtacct tggctcccag aagttccata cggtagacat tcaatgcctg tctgttggta
     1501 tcttcttttc ccatgaagat ttcgcattcc atccccagga aggctgctgc cgttgcggta
     1561 gccacaccgt gctgtccggc accggtctct gcgatcaggc gggtcttgcc catcttcttg
     1621 gcaagcagtg cctgtcccag ggcattattg atcttatggg aaccggtatg gttcagatcc
     1681 tctctcttga gatagatctt cgcgccgccc agatccttcg tcattttccc tgcatagtac
     1741 agcagagagg ggcgccccgc gtattcattc aacagcttgt tcagctccgc attgaattcc
     1801 ggatccttct tataatgctc atagcactct tccagatgga tcagttcatt catcaatgtc
     1861 tcggagatat attgtcctcc gtggattccg taacgtcctt tgctcatgtg ttcctcctct
     1921 tttctttcat cctgattttt attcctgatt tttattcctg attttcattt tgatttctga
     1981 tttataattc ttctatctat tgatgttggg ctcaaaagcc ccccgacttt gatgcctgcg
     2041 gcttgttccg ataccgttct accagttcca tgatccggtg catcttctcc ggatcttttt
     2101 tccggtcgct ctcgatgccg gagctaatat ctaccgcgta aggtcttacc gcttccatgg
     2161 cttccgccag attgtccgga tgcagtccac ccgccaggaa aaattctctg tcgattccgc
     2221 caaggacact ccagtcaaac gcctgtcccg tccccattcc gttatcgaat aacagatagt
     2281 ctgcactgct gtccagccag gcttccacat cataccggtc tctgacttta actgctttga
     2341 tcaccggttt accgcagacc gcctgcagat actggatatc ctcttctgtc tcatccccgt
     2401 gaagctgtgc cacatccaaa atatcttcct ccagcagttc tataatgttc ttcggatcct
     2461 catttacaaa cacacccacc gtccgaatcc ccggcagaag ctttttgcga tatttggctg
     2521 cctgctcctt cgtaaccacc ctgtggctct tcgggtaaaa tacaaatccc acgtaatccg
     2581 gctgcacctc attggcataa tcaatgtcct ccggacgata gagaccacac atttttactt
     2641 tcataaaatc ttctaccttt ctataacctg caaactttgg gccttaggca caaagttgcg
     2701 cgtgaaaaat ttatttttca cactgtccgc ggagttcctg caaagccgct ttcttatctg
     2761 cactacgcat caatgtctct ccgatgagta cggcattggt accgttctgc tccaaccgct
     2821 cgatatcctg tctcgtcttc ataccacttt cggatacgaa gagaatattc tccggcacca
     2881 tttccctcaa ccgcacggag ttattgatat ccaccgtaaa gtctttcaga ttccggttat
     2941 tgacaccgac gatccttgca tctgctgcaa gcgccatctt gacttctctc tcgtcatgag
     3001 cttccaccag tgccgacagg ccaagttctc ttgcaatccc cgcgtattcc gacaactgca
     3061 tgggagagag aatcgcacag atcaggagca ctgcatccgc tccgatattt ttcgcctcat
     3121 agatcatgta ttcgtctacc gtgaaatctt ttcgcagcag agggatctgt accgtctgcc
     3181 ggatcttcgt cagatattca ttcttccctt gaaaatatgc cgattccgtc agcaccgaga
     3241 tagcatccgc tcctgccgct tcgtattcct ttgcgatctg cacatatggg aattccggtg
     3301 cgatcagtcc cttggagggc gacgcctttt tcacctcgca gataaagctc atcctcttct
     3361 ttgccagtgc ctgttcaaaa ggaaatcccg tattgcagtc tgactctctt gctctggcga
     3421 tctgttctgc aagaggcagt gcttcttttg ctgctgccac ccgctccttt gcagaggctg
     3481 ctatagtatc cagtatcatc cattcatcct gcctttccgt ttcataatcg taatacttgc
     3541 accgcaggtc aaaatcactc gtaacacttt atcatgcatt gctcagcttg ataaaacgct
     3601 ccaactgtgc ctttgcaagg ccggtaccaa tgaccttctc tgccagcttc acaccgtctg
     3661 ccatactgtc tgccttgcct gccacatata gggctgctgc tgcattcagc agtaccgcat
     3721 tgcgcttggg acccttttcg ccatttaaga tagctctggt gatctctgcg ttctcctggg
     3781 gagttccgcc caccagatct tccttcgtgc atctttccat gccaaattct tccggtgtga
     3841 tcacataatt tttaaaggta ccgttatcaa tctcgcagac tgtagtgggt gcactggcgg
     3901 agatctcgtc cagtctgtcc tgtccataga ctaccatgcc acgttttaca cccagattgg
     3961 acaatacctt tgccatagaa ggcagcaggc tttcatcgta cgcacccatg atctgatata
     4021 ccggtcctgc ggggtttgcc agaggtccta aaatattgaa aatagtacgg atgcccaact
     4081 ccttgcggat gggtcccaca tatttcatgg cggtgtgata tttctgtgcg aaaaggaagc
     4141 agatgccgat ctcttccaac aacgtcttgc tctgttccgg ctctatggtg atatttacgc
     4201 ccagagcttc cagacagtct gctgcaccgg atttggagct ggctgcgcgg ttgccgtgct
     4261 tggctaccgg tacgcctgcc gcagagatca cgatagatgc cgtagtggag atattgaaag
     4321 aattagatcc gtcaccgccg gttcccacaa tctctaaagc ttccacgtcc gctccaagtt
     4381 ttctgccgaa ctttcgcatt tcttctgcgg agcctgtgat ctcttcgatg gtctcaccct
     4441 tctggcttaa tgctgtcaga taagcactct ttaaaatatc gctggcctca ccgctcatga
     4501 tctcatccat aacggttttt gccatatcgt atcctatgtc ctcattcttt accaacttag
     4561 caaccgcttc tttaatcatg tttctgcctg ctcctttcta ctacaatata tttatggtta
     4621 atatccctta cagccagtaa ggaat
//
LOCUS       DAWEJW010000155         4633 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5282_length_4633_cov_50.925295, whole genome shotgun sequence.
ACCESSION   DAWEJW010000155 DAWEJW010000000
VERSION     DAWEJW010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5282_length_4633_cov_50.925295"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1082 a         1294 c          937 g         1320 t
ORIGIN      
        1 ttttccagat caccggatag ctctcataga ccctctgaag ttccgcatca tctaccacgt
       61 agacctttgc atcctccgaa atcagatctt ttccgtattt cacagcctcc ggagtgatat
      121 tttccacatg atacagacct accgcaccgt tggaagcagt cgctgcgccg aaatccttca
      181 gataggtttt cgcagcatca tccagttcac cacctagcca tttgtccagt cctacgatat
      241 agggaacatc cgccatgact ttcataccaa tggcagatcc cagcagctgt gcttccggct
      301 ttttcgtggt ctcgatcttc acgatccagg tggcttttct gccttcatct gtcagcagtc
      361 cgaaatgagg cacataccct actaccgagc ccatgaggtc aatgataccg gagttacgat
      421 tgcatcttgc tcccaatacg gagttggcat aaacaactgc agaagattcc gaccatgaca
      481 gcacctctcc catagccggg gtatttccta cttcatccat ataacaggta caggtatacg
      541 catccttttc catgatacca agctgtgtca gctgcttctc gtagtcatcc tgcttggagt
      601 acatgaaatt tttgaacaca aaattttgta aaaaagaact ggggacattg ggatccagtg
      661 gtctgggatc tgctgaaaac ttctgtttcg atacattgcc ggattcgatc agctggttca
      721 tcagttcata caccagagcc agtgctttca atccgaagga tgttaccaga tggttgtatt
      781 tgctggtcac gggtaccatc ttatctgcgc cgaacagttc accgtaacgt accagtgtct
      841 gcatgacttt cgccagggtc gctccctgtt ttccatcata tatttcctgt tgttcttttg
      901 ttaagttcat tgatttccct cccccttaaa tcttcatggc aacttcataa gcgcgccata
      961 tgacacttcg cagattacct acaccattgc agtcgcctac gaggcttaca ttgctgccct
     1021 tcggtgccag tggattagga atgtatcctg cggagctgat cacgctgtca cagggaatcg
     1081 tgatctcttt tccggaagca ttcttgcata cgatgctgtc atcctttact tcctgcagcg
     1141 tagtctccag atatacgggt actttcttcc aggcaaacca ctctctcaga taggaactgt
     1201 tggccaggca tactccggtc tgggcgatga gatcgttttt catctctacg attacaggct
     1261 gtttgccctg caacgccagt tcataagcaa tctcgcagcc ggtgagtcca ccaccgatga
     1321 cggcaacctt ttctcctacg ggagtaccgg taaggtattc gcatgcctcg atcatcttct
     1381 catgacccgg tacttttttc agtactctgg gcacggaacc agtggcaaca atcacaggac
     1441 ttcctgcaaa ctgctcgata ttttccactt tttcattgta atggatctca atcttcagat
     1501 ctgccatctg cttccgatac caggttaaca gatcccgtag ttttccctta tagctttccg
     1561 cactggccgc aatgaaagta ccacccagat gatctgtctg ctcatggatc acaggttcat
     1621 gaccacgcag ctttagcact ctggcagctt ccatgccgcc gataccgcca ccgataatat
     1681 gtaccttctt cgggctggtg gtcgggacga tcttgtgctt ctcccactgc atggtctctg
     1741 cgttcaccgc acaccgagca aggtgcaggg aatccgacaa tgcctgatcg ttgggaactc
     1801 ctttataatg acacatattg aagcagccgt tgtggcacag aatgcagggg cggatatcat
     1861 cttcctgatc gttcatcagc ttcgtcaccc attcctggtc cgccaggaac tgtctggcga
     1921 atccggcacc gtcgattttt ccgtctgcaa tggcagccgc agctgtctcc ggatccatac
     1981 gtccggcgca taccacaggg atatccacga atttacggat atgcgccaca tcctccagat
     2041 tgcagttttc cggcatatag ataggaggat gtgcccagta ccaggcatca taggttccgt
     2101 tatcacagtt cagcatatca taaccggcat cctgcagata ctttgctgct ttttcggatt
     2161 ctgccatatc acggcctgct tctgtgaagc tttctccggg aagagctccc tcacggaacc
     2221 ccttggtctt ggattccaca ctgtacctaa gagacacagg gaaatcctgc ccgcagactt
     2281 tcttgatctc ttttacgatc tctacagcaa aacgatatct gttttcaaaa gatccaccat
     2341 attcatcggt tcgctgattc acatatttta aagtaaactg gtccagcaga tagccttcat
     2401 gtaccgcatg gatctccaca ccgtccacac cggcatcttt taataatttt gcagtcttac
     2461 cgaatgcggt gatgaactcc tgaatctcct ccacagtcat ctcccgggaa ggaactttat
     2521 ctgaccagcg gttcggagac gggctgggtg cggctgtaat cttgtcgaga tccataaaag
     2581 gtttcgccag tacccgcaat gccttattag tgtacagggt ctccatcatt tcactgatgg
     2641 taaacgatct gccgaaacct gccgtaagct gtacgaacag ctttgctccc gtcttgtgaa
     2701 attccggcat ccactttgcc agatcctcat acattttttt cttcttatac agccactgtc
     2761 cgtacatggg attgtatacc ggctgacagc ccggaagcac cagaccgcaa ttgttcttcg
     2821 ctacctccag gataaacttc gcaccatcct tgtcgaaatg gttcacttcc atccatccta
     2881 acaggttcgt gccgcccatg gatgtcagga caatgcggtt cttaatctcg cagcttccga
     2941 tcttccacgg cgtaaacaac ggttccaact gttgtttcat cgtaactcct cctttgcctt
     3001 ctctcttctc gtttgcgtac cccattacac agcttcagta taacatatcg agatataaat
     3061 ttgcgcataa aaatgatgtc ggggtcaaaa gcccccgact ctgatgccta cggattattc
     3121 tgccaaatag gtcacatttt gttgttgcat caacaacttc tttttatttt aacagactca
     3181 atgccgcttg tctactctgc cttccacagg tctccccata cggaatcaca caaattgaca
     3241 cagtattctc tggaaaaact ggaatcctgc aaaacgatat cattttcttc tgccagttct
     3301 tctgaataat tctccaaaaa ataagtcgtc acgccttcgg ggccggaagt cacatggctg
     3361 accagcggct caactccata gtccgcgatc tccacttcgc cgtcttcctt ctttgcgaga
     3421 atcacttccg ccataccgcc taccatgcga ttggcaacac cttctccggt accggaagtc
     3481 cagttcacga aatttcccag tgaataatag accagcattt catgctcgct cgcttcatct
     3541 gtcacccatt cgacgggttc gatcacatga ggatgcgtcc ccagtaccag atctgcaccg
     3601 ttttctgcaa aaactttcgt ccacttttcc tgggaggcat cgggtgtcaa ccggtattcc
     3661 gtgccccagt ggggacatac gatcgtgaaa tccgctagtt cctctgccct ctgaatatct
     3721 gccgccacct gttcctcact caacagatcc accgcatagg gtatatccgc cggcagagaa
     3781 attccgttgg ttccgtaagt gtagttcagg actgcaatcc gcatatctcc cagctcaatg
     3841 atcgcaggat ctgcaccgca ggaagtggag gtatccgcaa tatcatgaat acccaggaca
     3901 gtgatctttg ggtattcgtc tctccagtac tctgcacaat ttaccaagcc cgcacgcccc
     3961 ttgtccatgg catggtttgt ggcatgacag atgacatcga atcctgctcc caccaatgaa
     4021 tcgcagagac tgtaatccgc attgaaacag ggatacccgg agattccaag atccgcaccg
     4081 cctatgatca cctcctggtt caccagggca agatctgccg ccgcgatctc ctcttttgtg
     4141 tgggaaaaca gcgaatcata atcgtaactt ccatccggct gcagacagct ttcctccact
     4201 ggagtgtgaa gcagtatatc ccctaccatg atgatcttgt actccggcat agcttccgat
     4261 agctggtcgg aagatggctg gattgcatcc tgagtttctg tttctatttc ttctgtttct
     4321 attgcaatag ttcctgtggt actggcaata ttttcatttg ttactgtatt gtcctcagac
     4381 accacctcag tcttcgcaca gccgaacaat aaaaaaatgt tccctataca aatataaaac
     4441 attattgctt ttcttaaagt caaaaatctt ctatctgcca tttttctatc ttttcactga
     4501 tttttctgta tttttttctt cttactatgt cccattataa catacctatg atttcggggt
     4561 caaaagcccc cgcctttgag ttcgggtaaa tttactgtag gaaattaata gacagagcat
     4621 ccttgagata tgt
//
LOCUS       DAWEJW010000156         4544 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5391_length_4544_cov_65.605480, whole genome shotgun sequence.
ACCESSION   DAWEJW010000156 DAWEJW010000000
VERSION     DAWEJW010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5391_length_4544_cov_65.605480"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1215 a          974 c         1266 g         1089 t
ORIGIN      
        1 gagagccggg ccgttaaaag gaaagagggg ccagaagtag ctgaagcccg cgaaggtcgg
       61 ggtagtcaaa atagagactg tcaccagtgt cagaccgata aggaatcccg gcaatcccca
      121 gagccctgtt gtgacgacca aaagaatacg gtagatccgc agagcatcag caaattcgat
      181 actggagagg gacagattcg ccagcatggt gacagcagca tagaagagga cttccgtgga
      241 ggcccagtta agactgacag caatgtcacc gattaatagt ccgccgacaa tggacagagc
      301 accggagacg cggctggagc tgagtgccgc tgaatacttg aaaagatcca gcaggaactc
      361 caccgccagc acatagaaaa aaagacggaa gggtgacaga tcgctgacgg gtgtgagctg
      421 cagcgaggca gtaatctcgg gatagtaggc ggagagaagt aaaaataccg gaaggagtag
      481 gagactgacc ggaatgcaca ggaagcgtat catgcggaaa taagttccgg tcagaggatt
      541 gttgtaatag tcctccggac tctgggtaaa ctggaagatc gtaccgggaa gcagcagaac
      601 ggcaggggag ttgtccacga tgagcagaat atgcccttcg cacagatagc tgcaggccac
      661 atcgggccgt tccgtcagct ggatactggg cagaggattc caccagcgtt ttttgatcag
      721 gagttcttcc agacttttgc tgcccatggt cagggaagtg atctgtaatt gggaaagctt
      781 tttctttaag gtatctaata attcctcatt gacctgatcc gccagatagg cgatagctac
      841 atccgtgcgg ctttctgcac ccagagaaca gatagagaat gtcagtttcg gagaacgcac
      901 cctccggcgg atcagattgg tattgaacaa caacgtctcc acaaaaccgt cccgggcacc
      961 acgggtgatc cgttcggtgt caggttcgga aatgctgcgg acaggatagg tacggacatc
     1021 gatgattacg gcctgtgcaa aaccttctac cagaaggaca gaggggccac tgagaatatt
     1081 ttgcagaatc gtatctatag aggaagtcag tgacacctgg gcatacccca gtttgctctg
     1141 cacatagcga gggagatccc ggatttcgga atccagtgta tagagggggt cctgcaagtc
     1201 ggaaaaaatt tgttgaagga tctccgtctt acaaaaacca ttgacaccga tccagtaagc
     1261 cggcgtattt cccagcttca ggtcacgggt gatcaggtca aaactgtcat ccagtggcag
     1321 cagagcgtgg aaaagtgctg catttttgga tagatttgtg gataaggagc gttgtaccat
     1381 agtgggaaat cctttctgat cacttgcata gactgccaaa caggtatatg gtattattat
     1441 tcagatagat tccccaggtt tttcggaact atacggatca gtggtataat tgacacaacc
     1501 ccagcaggat caacaataaa cctgagacgg gatccgtata ttccccgatc tgctgcagga
     1561 aagcagacct gcccagacgg cttccgaaag ccagaaatcc gaaggagcag aaaaaggtgg
     1621 acaccgatgc cggaaggaaa ggaagtcccg taatactggc ggagaaaccg atgccgatat
     1681 tgttcatgga aagggcacag cccagcgcca gtatctgcga caggaataat tcccggtgcc
     1741 cgacaaattt tttctttttc tggcacagat tattatccgg ggaaaaattg cagcaccatc
     1801 tggaaagcag ccattttccc acgtagtaaa gaccgaaaag gatcagtaca ctgcttccga
     1861 tccgggtacc gatgccgatg ggaaacaagg gtaacaacag gttgccaaga cctatagaga
     1921 tcacagtccc gatcagggtg actgtgctga tcagcagatt atgtctggca ggcagaggga
     1981 ctttctggag gcccagagcc atacctacca gaagggcatc cagactggct gagatgccga
     2041 agagcagagg gggcagaata gacataacat gctccgtaga aaatattctt aattttacta
     2101 tattcccctg aaattatttt gtgaggttgt ggggcaaagg aacaaatgag tatggaagta
     2161 aagagaaaac tgagtgaatt cctaaaaaga gagacggttc tgaccgtagc gatcctgctg
     2221 gcaatagtgt ccgcggtgat gattccgccg gacagagaat atgcaggata tattgatttc
     2281 cgcacacttt cgatcctttt ctgcctgatg acggtgatgg caggcttgca gaaactgggt
     2341 gttttccgta atattgcaaa aacgctgttg cggcatacac agaataccat acagttggca
     2401 gaggtattgg tacttttgtg tttcattttc agtatgatca tcaccaatga tgtgtcattg
     2461 atcacgtttg taccgtttac cttcattgtg ctgcggctga caggcgaaga agctgtgaaa
     2521 aaactggcag tacctgtgat cgttctgcag acgatagctg ccaatctggg aagtatgctg
     2581 acgccgatcg gaaatccgca gaatctgtat ttatatggga aaacacagat gtccgctgga
     2641 acatttatac ttttgatgct gccttattct ctggtgtcac tgcttttact gatgatctgt
     2701 gtggtaattg tggcaaaacg aagcggaatc gaagtccggg gagccgaagt tttgctgaca
     2761 gaagatgaga agctggaaca aaagaaatat ttacttccgg catatttgct gttatttgtg
     2821 ttgtgtctgc tgactgtggc tcatatgatt ccttatccgg tgacattggg aactgtggca
     2881 ttggcggttc tccttttaga tcgtggtact cttataaaag tggattattc gcttttgctg
     2941 acgtttgtgg gtttctttat ttttatagga aatatgggaa gaatgcctgc tttctgtgat
     3001 ttcctgcaga agatcatcgg aggtagagaa gtgatgacag ccgtgattgc cagtcaggtg
     3061 atcagtaatg ttccggcggc cctgctgtta tccggattta cggaaaatat aacggcattg
     3121 atcatcggta cgaatctggg agggctgggg acgctgatcg catccatggc cagtctgatc
     3181 tcttataaac aggtggccag acagattccg ggagagaaaa agaagtattt tggatggttt
     3241 accatagcaa atatcgtttt tttaatgata ttggtattgg aaaactgtct gctgtaacta
     3301 tagaaagcag tctgtctgcc gggaaaggca gaaaagatgg caataactaa aaaataatct
     3361 gggaggtatt cgtcatgaca gaagatatga caatcattgc agaagaggct tatctggcgg
     3421 ccaaggagat cttggaaaag gcgaggctgg agcagggaga gctgtttgtg gtaggctgtt
     3481 ctaccagtga ggtgggaggt gctactatcg gtacctattc cagcccggag ctggcggaga
     3541 tggtcttcgg cggtatttac caggccacgc aggaagccgg ggtatatctg gctgcgcagt
     3601 gttgtgagca tctgaaccgt gcactgattc tggaaaaaga ggctgcggta aaatatggtt
     3661 atcccatggt aaatgtggta cctcagccta aggcaggcgg atcttttgcg acggcggcgt
     3721 ataaagcctt cgagcatccg gtggctgtgg aacatattca ggcggcagca ggaatggata
     3781 tcggggatac gctgatcgga atgcatctga gagatgtggc agtaccggtg cgcctccggt
     3841 ccggacagat cggagacgcc catgtggtat gcgcccgcac cagaccgaaa ttcatcggtg
     3901 gcggcagagc ggtttatgac gaagaattat tgtaaagaaa tgatatgtgg ctatttctgt
     3961 ggcattccgc ggaagtagtc acggatccag cgtccggatt cattgaaatc atcgtcggtc
     4021 cagtcggaaa gtgccgtgct ggaagccttg aagacgctgc tgctttcgtc tttgttggcg
     4081 agattccagc aacagaagct gatctggtac ttgttcagca gatccagcca tttggtggtg
     4141 gaaacaaaat cattggcgcc attgccggag gcatcgcaca tgccaaattc gctgacaaat
     4201 accggaagcc cgttctgaac acaggtctct aagcggttcc gcaggtcgtc tttatgggtg
     4261 cctgcgtaga aatgcagcgt atacattaca ttgctgtcat ccagcggaga agcggcagcc
     4321 ttgtcgatct cctgactcca ggttggtgtt cccacgagaa ttacggcatc cggcttctgc
     4381 gcccggatca ccgggatgac ttcttctgcg taagacttga tgctgtccca ggaagtaccg
     4441 ccgttgggtt cattgcagat ttcatagagc acattgtcgt tgtcggcaaa aacttccgcc
     4501 atttcccgga aaaaggcgat cgcctcatct ttgttctggt tcgg
//
LOCUS       DAWEJW010000157         4383 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5592_length_4383_cov_61.250000, whole genome shotgun sequence.
ACCESSION   DAWEJW010000157 DAWEJW010000000
VERSION     DAWEJW010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5592_length_4383_cov_61.250000"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1211 a          955 c         1210 g         1007 t
ORIGIN      
        1 catcttccgg cagcagtatg agctccatga tgagcaccag cagctatgag atcgatttac
       61 agggtaatga cagggcagaa ctggcacagg ccgcagaaga cctgcaggca gagatcgaga
      121 agattgacgg tgtcgtaaag acagaaaact ctctggcaaa ctctacgacg ctggccaagg
      181 tagtggtaga tcctctgaag gcaatgcagt acgggctgac acccattcag gtcggcatga
      241 ccatacataa cgtattaagc ggtatgaatc cgttgacgat caccaatgat ggttcggagt
      301 atgcggtatg gctggaatat ccggaaggaa gctatgatga tctgaacctg ctgatggatc
      361 tgggactgga tacttctttt ggcaccgtgg taccgttaag agatattgca gagatcaatt
      421 acgcacagag tcaggagacc atcatgaagc aggacggtct gtatcaggtc agcgtgacgt
      481 ccactatgac ctccgagaag atatttgatg cccagaatgc tatcaacgat ctggtggatc
      541 acacggtatt cccggattct gtaaccccgg cagacagtat gatgactgga atgatgaacg
      601 atgagttcca ggcattgttg caggcaattc tggtagctac attcctggta ttcctggtga
      661 tggcaatgca gtttgaatcc cccagattct ccttcatggt catgatgtgt attccttttg
      721 cactgatcgg atccttcctg ctgttgttca ttacgcaaag taccattagt atggtatccc
      781 tgatgggatt tctgatgttg atgggtattg tggtaaataa cggtattctg ttcgtggatt
      841 ccgcaaatat gctgcgggaa gagggaatgt ccactgagga tgcgctggtg gcttccggaa
      901 gtacccgtct gcgtcctatc ctgatgacga cattgacaac gatcctgtcc atgatcccca
      961 tgggactggg actgggcgat aacggtgtca tgatgcaggg aatggcactg gttatcatcg
     1021 gcggtctgac ggcttccacg atcctgaccc tgatcctgat cccgaccttc tatctgatct
     1081 tcgataagcg gagcagacag gagagaagag cggctaagaa gctggcaaaa tctcaaagat
     1141 ctggaaaaac gggggatgcg gaaaatgagt aaaataaccg gattggaggc accgaaccag
     1201 gtgcctccca aggtgaccgc catgtaccgg gccgtcagca cacttttgcg ggaggacaag
     1261 gatatctcgg aaatgagcgt gtccatgatc accgggctgg cgggcattgg caagggaact
     1321 gcttatgaat attttgattc caaggaagag atcattgtct gtgccctgtt gtatgaaatc
     1381 cgtacagtaa cggagcaggc ttcccatcag attcagacct gtccggatct ggagacccag
     1441 atccacagaa tgcttttgct ggtggaagaa cattcccagt gtgtggatgc catcatggcg
     1501 tttctgcatc tgctgacgga tcattccaag gagggaaatc ttctgcggca acggatagcg
     1561 gaacagaagg aaaacgggcc ggtggatctt ttggtccgcc agatcataga cagtgcacgg
     1621 gcgaaaggag aactgcggga ggacattccg ctgtcttata ttaccaacgc gctgttagct
     1681 cggatgattt cctatctgat gtaccagtgc caccatatcg gtgatgatat gcccccagaa
     1741 gagatgcgca gactggtcac cgaaagcata atgaatgaat tgtgcaaaaa aaatatataa
     1801 tttaaaataa ttctattgac aaagtgagat acctttgata gaatataaca cggtgattgc
     1861 gttagcagtc acgtgatgct gctgtggctc agtcggtaga gcgtcgcatt ggtagtgcgg
     1921 aggtcacggg tccgattccc gtcagcagct cttttgaaaa gcgtggaaac cttgaaaata
     1981 caaggtttcc actttttttg tgtgcctgaa agtttacaga cgatggtaga caaccagaat
     2041 aaaccagatg tacacttgca cgggccaacg attcagctta caatataaaa tagtaaattt
     2101 atgttcggac acaaaccaac ccggtcagat gaggaaaacg tatggaggaa ctgcacagag
     2161 tatgtaaaaa tttcacacgt catgacaaga agacccgtac gatcctgctg tgcgagatgc
     2221 tggagtacac gaatgtattt ttggaggcgg cattccgggc ggaggggtat tccttcgaga
     2281 ctttaaagaa cccggtgaag gaccgcactc tggcacttcg ctatatcagt agcgactatt
     2341 gctatccgac ggtattgatc cttgcacagt tcctggaata tctggaaagc ggggaacggg
     2401 atccggagga gatcaccttc atggagcccc aggcgggcgg tgcctgcagg gccggaaata
     2461 tctacaatct gctccagaga gtgctgtacc gcatggcaga gcagggacag ctggagtatg
     2521 cccagattcc cgtaatctcc ctgaacctca tgggagaaga aaaacataca ggctttcaca
     2581 ttactccgtc actgttgtcc ggtgggattg ctgccgcatg ctatggcgat ctgatcatgt
     2641 gcctgtacca acaggtgaag ccctacgaac agaatccggg agagacggac cgcatccgca
     2701 gtcaggtgga aaagaaactg tgcgagagca ttcggagaca caggaacgga cggcacacca
     2761 gagagaaaaa ttaccggtat gcctgcgaag tattttcgaa ggtaccggtg aaacccggga
     2821 agaaaaagag agttggcatc accggtgaga tctatatgaa atattcgaaa ctgggaaatg
     2881 acgatatcga aagttttctg ctccggcagg gaatggagcc ttacagtggt ggtttcgtca
     2941 actactgtct gtatttggtg gatacagagc ggtataaggc agaaaagtac cagcacaatc
     3001 ccatcatggc gaaagcctgt gcctttgtgc tccaccggtt gtgtgaggca caaaaacatc
     3061 tgcttacggt gatcggggag acagaccggt tccagacgga tctgccgttc ccggaactga
     3121 aggagaaagt gcaggggatt ctggcggagg gctgcaatac cggagacggc tggctgattg
     3181 ccgcagaggt gatacaatat gtggaaaaag gctgtggcag tgtgctgatc ctgcatccct
     3241 tcggatgtct ggtcagtcat gtctgcgagc gggggatttt gcagcggctg catgcccgtt
     3301 ttccggatgt cagtatccag accatagagt atgattacga ccagagcaag accctcagag
     3361 aaagccgcat tttattggca atttcctagg ttggcagcag gtgcagaacg tggtacaata
     3421 aaacagaaac tattgcaacg tgtattttca ggaggggaac atgaagataa aatgcgactg
     3481 gtgtggcagc tggatcaacg attttgatca ggtctgcccg aactgtggcg gtgtcaacaa
     3541 caattacaac cgccatgcca acggtgtacc acagacaatc gaggaactga aagcctgggc
     3601 aaaggagatg aaccttccgc tggaggacat gcggaccttt atcggagagg attacaaggg
     3661 cgcgaaagcc ttcggtattt acaaggatga gaccgatggc accttcgtag tctataagaa
     3721 taaagaggac ggtacccggg cagtacgtta caagggaacc gatgaagcct atgcggtcaa
     3781 cgaactttac cagaagatga aggaaagagt ggcagtgcag aaagcacacc agcagccgaa
     3841 agctgcggca cctgccagcc gcccaaccaa agattacacc gcctataata agaaaagagc
     3901 ccggagcaaa aagatagaaa aaacggttct gacaattttc tgcctgatca tggcagcatt
     3961 gtctctggtg gcgattttta caggcgccta ttctatctgg cagattgaca acagaccgga
     4021 taacggctat tacacctatg aggatcagaa ctattactat tatcatggga actggtacga
     4081 atgggagaat gattcctggc gctatgcacc ggatgaatcc tggatggaaa gcgattacga
     4141 gagttactat gattcctaca gctatgacag cgatgcggaa tatgaggatt ttgaggattc
     4201 gtcctattac actccttatg agtacagcga tgataataac gatagctggg atgatgacag
     4261 ctgggattcc ggatgggatt ccaatgacag ttgggactca ggatacgatg actggggctc
     4321 tgactggtaa aatgtgattt agaatacata caaaggaagt cttcgacagg cttcctttgc
     4381 ttt
//
LOCUS       DAWEJW010000158         4277 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5751_length_4277_cov_65.785410, whole genome shotgun sequence.
ACCESSION   DAWEJW010000158 DAWEJW010000000
VERSION     DAWEJW010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5751_length_4277_cov_65.785410"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1121 a         1092 c          933 g         1131 t
ORIGIN      
        1 acgcaaaaaa ccgctgcccc ttacggggca acggtttttg tattcaaagg attatccctc
       61 aatagcgatg tatttattgg tctctaccgc aggctttgct tctttcttag gaacattcag
      121 agtcaggata ccatgtttga actcgccctt gatatcctcc tgagtcaggt tgtcaccaac
      181 atagaagcta cgctggcaag ctccggcata acgctccttg cggatgtatt taccggtctt
      241 cttctcctgc tcatcctcat ccagaccttt ggcagcctca atggtcagat aaccatcctc
      301 cagagaaacc ttgatctcat ctttcttgaa gccgggcaga tcaacgatca actcatagtt
      361 gtcatcggtc tccttgatat cggtcttcat tacattatgt gcatgtttgc catacagttt
      421 cttctctatc tgattctctg ctctgttgtc aaagaaaggt acatcatcaa aaaagtcatc
      481 aaataagttc tctccaaaaa tgctaggtaa taacataagt catctctcct tttctattaa
      541 acatatgtcg gtgtctttcg atacctctta ccttttgtcc tccggcaacc gtcggagaat
      601 ttcggaacca gtcagaggat cttcgatcaa tctcttgatc tttcttcctc tcccttgttc
      661 tagttgtact ataacacgca tatataagta tgtcaattaa ggaaaaatta tataattata
      721 aattaaaaat aaactgcaaa aaagaaccag aacactgtga ttcacagtac tccggttctt
      781 ttttacaggc aaaaccgcca gtcttactta tttgttttct cttacttatc ctttgcagta
      841 aagaatacga agaatacatc tccctcagtg tcattggaga actgctccag ataagaaatg
      901 gaataatcct tggaatcttc cggtacttca tataccagca gaccggtttt ctcttcgtta
      961 acacccagat catactcttc cggcagctgt tcgtcactta cagtgttacc atagtaggtg
     1021 ataggtgcag cgtatgcgtc atcgttatca tcatcgcccc actggatctg gaaatcggtg
     1081 tcatacatgg taacggtaga acgatcggtg ttcttaacgg ttatttctgc tactaacagc
     1141 ttatttccct cggaaggggt atatccttca tagtcattgc aggtatatgc gctgtttaca
     1201 gtgtaatcga agaagtaagt tctcattaca tctcccatac gtccttctgc atagccgtca
     1261 tcatctgcgg ttaccacacc actcttgctg ctggtattgg atccggaact tgcaatattc
     1321 tgaattgcgc tgtctacagg accacatcct accagactaa atgccagtac agttgctaca
     1381 ataattccaa caaatttttt cattttcttt ctccctctca taacccttat tgtgcatgca
     1441 gtcatctgca cgcaaagcca gtatagcaca caaaatggca gaatgcaacg ctttattgcg
     1501 ctactttgcg aatgtaagcg cttactcacg ggtcagacgc aggatcacat cgctgtgttc
     1561 cggatatttt tccaatagtt ttttccgttc aaacagttca aattcttcat atttaaaaca
     1621 gggtgaaacc atacagccca ccagactgaa accctcttcc tccatggcag acccgaagat
     1681 cattcccttc ggaaccagta cctgcgggac ttctcccttt tccaggtcca gcccaagttt
     1741 cgccgctgtg agttttccgg cctcatcgat catataaatc gtcaacgcat tgccgtcatg
     1801 ataataccat acctcatccg acttcaggcg atgaaaatgg gacacttccc cctttgccag
     1861 cataaaataa atgctgctcc acagattcct ctcttctttt tccagcaggc attctttgaa
     1921 ataaccgccc tccacatggg gctccatttc cagatgctct atataatatt ccgctgaatg
     1981 cttcatactc ttatccctcc ggctattttc ttttctactg aattagtgta tactatgcag
     2041 agaaaaagcg caattattaa cctgaaagat tgtgcggaat ggagaatatc atggaaaaaa
     2101 tagcaagctt caccattgac catatcaagc ttcagcccgg tgtctatgta tcccgcaagg
     2161 atcacatcgg tgcagaggtc atcaccacct tcgatctgcg tatgaccagt cccaacgaag
     2221 agcctgtgat gaataccgca gaagtacaca ccatcgagca tctgggtgca actttcctgc
     2281 gcaatcatcc ggaatacaag gacaagaccg tctacttcgg ccccatggga tgccgtaccg
     2341 gcttctatct gctgctggcc ggagatctct cttctaagga tattgttcct ctgatggtgg
     2401 aaatgtttga attcatccgc gattacaagg gtgaggtacc cggtgcttct cccaaagact
     2461 gcggcaacta tctggatatg aatctgggta tggccaacta tctggcaaaa cgctatctgg
     2521 ataatgtgct gtacggcatt gacgacagcc gtctggtata tccggagtga ttacaattat
     2581 ttaagaaaga acaggaaaca cacatgaaaa aaatcggaat catcggtgcc atggagctgg
     2641 aagtggaaac tctcaaggct cacatgacca ccactaatat cacgaccaaa gcaaatatgg
     2701 aatttcacga gggtaccctg aacggtactc ccgtagtcat cgtccgcagc ggtgtcggca
     2761 aagtcaatgc cgctctctgc gtacagattc tggcagacct ttttcaggtc actgccatca
     2821 tcaacaccgg tgtggcagga tccctgaatg ccgctctgga catcggagat attctgatct
     2881 ccaaggatgc cctgcaccat gatgtagatg ccaccatttt cggttacaag cctggggaag
     2941 ttccccagct gggatgccgg gaattccttg cagatgagca tcttatcgag gttgctgcca
     3001 acacctgtca ggaggtcaat ccggacattc acgttcatac cgggcgtgta gtcagcggag
     3061 atcagttcat ttccagcaaa gaagtcaagg accgcctgat caaagaattt cagggggact
     3121 gtgccgagat ggagggggct tccattgccc acggtgctta cctaaacggt attccctttg
     3181 tcatcatccg tgccatttcc gacaaggcgg atgacagtgc cgagatggat tatcctacct
     3241 ttgaaaaggc cgcagcgctc cactccgcca agctggtaga ggctatgatg ccccggatct
     3301 gatccgggcg gctgtcctgt ggatcgaaaa caatgcctgc tgcacagact ttgcaggcaa
     3361 tagaaaggca tgcatattaa catgaaaaaa gaaaagaaat acattgaata tatcttaaaa
     3421 caggccgaag ccctgctggc tatcgacagc ccttccggct tcacggaaaa agtaacggat
     3481 tacctctgtg aagagtacaa gcgcctcggc tacaaaccgg tcaagaccaa aaagggcggc
     3541 gttctcgtag agatcggcgg taaaggcaac gcggttctga ccatggccca tgtagacacg
     3601 ctgggcggca tggtggcaga gatcaagatc aacggccgtc tccgtctgac taaggtcggc
     3661 ggcttaaacg ccaacaatat cgagaccgag aactgccgta tctatacgct ggacggcaaa
     3721 gtatacgaag gcaccgtaca gctgacggat gcttccatcc atgtcaacgg cgattaccag
     3781 aagtccgagc gtaccttcga ttccgtggaa gtcctgctgg atgaaaaggt atcttccagg
     3841 gaagatgtca aggcactggg tattgagaac ggttccttcg tctgcatgga tccccggacc
     3901 cgcatcacgg agtccggtta catcaagagc cgttttctgg atgacaagct cagcaccgct
     3961 attctgcttg gcttcgcaaa atatgtaaaa gacgaggatc tgaagctgtc ccgtaaggtc
     4021 taccagcata tcacggtctt cgaggaagtg ggtcacggtg cctgcgcttc cgctccggaa
     4081 gatgtggagg aactgctgtc cgtagatatg ggttgcgtcg gagagggtct ggaatgcacg
     4141 gatcgtcagg tatccatctg cgtcaaggac ggccacggac cttatcatta tgatgtggtc
     4201 aaagggctga tccaggctgc aaaagacaat catatcgact acgccgcaga tgtttacccc
     4261 tactacggct ccgatgc
//
LOCUS       DAWEJW010000159         4260 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5775_length_4260_cov_70.136266, whole genome shotgun sequence.
ACCESSION   DAWEJW010000159 DAWEJW010000000
VERSION     DAWEJW010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5775_length_4260_cov_70.136266"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1001 a         1160 c          881 g         1218 t
ORIGIN      
        1 agaaccggaa acaagataga aatcataaat gtatggttgc gtatttcatc acatctatgt
       61 tacaatgttt tttaggaatt tgttcctatt aactgaagga gggactgtaa aatgagtccg
      121 gtggcaatca cattattagt tattgtaata gtacttgcga ttatcattgc agtattatat
      181 ttcttaggta agaaagcaca gaagaaacag gcagaacagc aggaactgct ggatgccaac
      241 aaacagaccg tatccatgct gatcatcgat aagaaacgca tgaagctaaa agatgccggt
      301 cttccacaga tggtcattga ccagaccccg aagatgatgc gcggttccaa actccccatc
      361 atcaaggcaa aggttggtcc tcagatcgta tccctgatct gtgacgagaa gatcttcgac
      421 agcgttcctg tgaaaaagga agtaaaagcc gttgtcagcg gtatctatgt actggatgtc
      481 aagggactgc acggcaagac cgaagctgct cccgagaaga aaaaaggctt cttcaagcgt
      541 cttgtagcta cggcacagga aaaggccgga gcaaagcctg taaagtaaat aactattcag
      601 accccattgt gtgtcgtctg acgcacataa aaatagcaga tgttcgttga atgcttgcat
      661 tcataagagc atctgctatt ttttatgttt atctttcttc tatttttccc gaatatggat
      721 cttcaggcag ctttcgctca ggggttctcc ctcatgatcc agcgtgtatt ccaccgtcac
      781 gtgaatctga tcctcctgtt cctctaccag taccctgccg gtttcgatcc ctagcatcag
      841 ttgcccgtag ggtgtgttgt aggggatcat atgtctctta ttttcctcga agatcatatg
      901 catctcagcc gtcccctgtc tggtcagttc caacagatga tcccggaatt tgatccggct
      961 cctgcaggga gcttggtctt catccatttt ctcctcatag agcagataat gactgtcgtt
     1021 tctcttaaaa tattgtgccg gctgcacggt ctccaccgct tcgctttcct gctcctgtcc
     1081 ggaagcatgc agaccggaca gtacaagttc tacctctcga ttcattttct cttcatcctc
     1141 taatactcat cgatcttaat ggtcttggcc gacaggattc cgtatttaat aatggaattg
     1201 gcaaagtgct tgaacttctc cgccccatcg ctgacataga actggtactt gttctctccc
     1261 agatgcggct cctcatcatt gagaatatga taatactgta acagttcctt cagttctctg
     1321 gctgtctcgt atgccggatt caccagcgtc acctgatctc ccatgacacg tcccaccgtg
     1381 gaacggatca gcggataatg ggtacacccc agaatcaggg tatcgatgtc aatatcaatc
     1441 aactctgaca gataacgctt tgcgatctca tcagtcacgg gatcttccca caggccttct
     1501 tccactaaag gtacgaacag cgggcaggcc ttgccgtaga tcgtaacatc tttattcagt
     1561 tcctgtatgt atttgttgta catctggctg ccgatggtag cttcggtggc aatcacgccg
     1621 atgcggccgt tgcgtgtcac ctccgccgcg gttctggctc ccggcttgat tacaccgatg
     1681 atcggaacat cgatctcttt ttccagttca tccattgcat aggcactcgc cgtattacag
     1741 gccactacaa tagtcttcac ctgaaaagtc tccagaaacc gcacgatctg ctttgaaaag
     1801 cgggtcaccg tctccttgga cttactgccg tagggcattc ttgccgtatc tccgaaatat
     1861 atgatcttct cattgggaat ctgccgcatg atctcccggg caacggtcag tccgccgata
     1921 ccggaatcga ataccccgat gggggcatcc tttttcagcg cagtaacatt ctcctgatac
     1981 atgggatttc tccttttatt tttttaacat cgtaagcagt ctgctcttga accggatctg
     2041 ctccggatcc gcctccagaa tcttccaata gatctggctg tcggaattcc attctaccac
     2101 ttccggatcg gtctgtacag tctctgcctc cgtggcttcc gtctcctcgt atacgatccc
     2161 ataggtggac ggcagcatcg taagctccgg gtagatcacg ccccaccagc cgtaacaggc
     2221 cagtgccaca gtcagtatcc ggactccctg caccagccgg tgtaatattt tcttttctgc
     2281 aatcatcctg cgatattttt cataagaaac atataatcta tccatcagca ttcctctttc
     2341 cataaattac cgtttcggtt ctatggaaat cattaccgct ttccggacag attatacttt
     2401 tgaaatctat ttatttttct cccgttccag acggatctgg cggatgatcg cctttttctg
     2461 attatctata tgcaccttgg ccatattgga tgccgtttcc ttatcctttt tcacgatgct
     2521 ctcatagatg accttgtgtt cctccaccag gcgctcatgc tggctgaaat ccttgatata
     2581 ctcaaaacga taacggtaca tctgctcgga caggttgttg accagctgga tcaggcgctg
     2641 gttccccgtg gcctgtacaa tgatgtcatg caatgccaca tccgcctgtg cgatctgttt
     2701 aatgtcatcg gtcttcaccg ccgcttcaaa ggtatcgcag gcctttttca gatcctgtaa
     2761 ttcctccgga gtgatccggt cacacgccag ctccacgcag agcgcatcca tggccctgcg
     2821 gacctccagg acatcgttca tgctcttctc cgtgatctga gccacttctg ctcctcttct
     2881 ggggatcatg gtcaccagtc cctccagttc cagcttacgg atggcttccc ggatcggcgt
     2941 acgactgact cccagcttgt tggcaagatg gatttccatc agtcgttctc ccggcttcag
     3001 ttcccccgtc agaatggcct gacgcaaagt attgaacacc acatctctga gtggcagaaa
     3061 ttcatccatt gtcacctgta aattatcctg catacctcta ctctggcatt actcacaaaa
     3121 ttccgtcagg aaaatctgct ttgccagctg ttcctcctct aatttatcat atgccctgcg
     3181 ggccatgtcc ttctctacaa aaataccgaa taccgtggga ccgcttccgc tcatcagact
     3241 gttgatggct cccagctcct tcatacggtc cttgatcgtc tggattacag gatatgcact
     3301 gacggttacc gtctccagca cattttccat ccggtccagg attccctgta agctttcctc
     3361 agcgatggct tccaccatac cgtcaatatc aggatgcttc acgatctcct gagcatccag
     3421 atgttcgtac acgtatttgg tggaaacatt gatatcgggc tttgccacca gtacatagca
     3481 gtccggtgcc ggagcaagcg gtgtcagttt ctcgccgatt ccctcagcca gcgccgttcc
     3541 gcccatgacg caatagggaa catccgcacc gatcttcacc cccagttcca tcagatcctt
     3601 cagtgtgcac ccaagatcaa acagacggtt cattcctttt aaggtagctg ctgcatccgt
     3661 actgccgccg gccatgcccg ctgctatggg aatatgcttc tccagatgga tcttcacgcc
     3721 tccactgata ggataagtct ccatcatcag ttttgccgct ttgtagatca gattattctc
     3781 atccgtaggc agttcaccgg aatctgtggt tatcacgatc ccaccgtctg ttctctcaaa
     3841 gtccagcaca tcatagattc ctacagtctg catgaccatc ttcacttcat ggtaaccatt
     3901 ctccagtctt ctgaccacat ccagccccag attgatcttg gcatacgctt ttatccgata
     3961 ttcgttcatt tttgtctcca atacgttagg acgctattcc aaaactacat gcctctgaat
     4021 agcgtgtata caataattgt acttaatttt atacaatcct gcgtattttg tcaatggagt
     4081 gcagttcttt ccatagattt ttccatagat ttttcccctg tttttctcta aatcctctaa
     4141 agtatccact gtccggggac gatataacag gtgaaaccaa aaaacctcag aaggttaaag
     4201 catgtatagt caaggatgac aacaggtacg gacagcagaa ccgtaccgga aaagaggaaa
//
LOCUS       DAWEJW010000160         4224 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5820_length_4224_cov_63.667066, whole genome shotgun sequence.
ACCESSION   DAWEJW010000160 DAWEJW010000000
VERSION     DAWEJW010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5820_length_4224_cov_63.667066"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1293 a          882 c         1105 g          944 t
ORIGIN      
        1 ccacagagtt ccgataccga agctcagcaa acacagaaat cccagcggca ggaaactcat
       61 ttgtaagcag aacagctccc atttatgtcc tttcataata cggaagctta atttcatcag
      121 ctccatagcg ctatactggg gaaaatccaa cagtaaatag taagcctggg ataaaccaag
      181 ggataacgga atatagatac acataccgat gaccataagc acaatgcaca ggattgccat
      241 ggttatggcg tcaaagcctt tccctgaccg caggagaaaa tagcagatat caaacggaag
      301 tgtacatgcc gtattcagca ggatcagtac tgcggagatc ccgagcgatt ttttgaataa
      361 gtatttgaag ccgtagaaca gattggcagt tacagcaggt ctgccgcagg cagtattcag
      421 ataaaataat gtgatgccgg tctgaaatac tccggtgaaa atcgacaata aagtcgtaca
      481 ggctgtcaaa agcagatatt ccgtcagaga tgtctctgcg cttcccccca taagtgttcg
      541 tacggtaagg atcatagtga aggtcatgct gacggcaaag gtgagggaaa atacgatcag
      601 actccggcag atggacagca ggatggcatt gccgtaatgc ccggacatct gatctctggc
      661 tttttctttg agttgagctc tggatgcata ttgtatcata gaaatcctct tttcttttta
      721 ttatttcgtt ctattggtta ttccggctgc tccgggtttt gatttcgtaa ccgcagaatg
      781 catcggaaaa cataggcatg tcttacaggt aagattgcca gaattcttcc atatcgacac
      841 cgtacatctg ctggtacatc tgatccagca tattcctata agaaggatcg gtaaataccg
      901 tgacaatgct gctgatcgtg atgaagatac cgagtgccag tcccacacag gacagccaga
      961 tcccggcaac acacatattg ttcatgcgtt gccctctttt ccgggtgagc aatgcaaaga
     1021 gaatgcccag agcaccggtg ggaatggaca gcagtcccgt gcagcataat accagggaac
     1081 agatgccgca gaccatagaa gcgatcgaca aacccctgta tttcggatcg atctgtggta
     1141 tattttgatt catttcgtta ttctgattct gataataatc catagaaagc ctcatttccg
     1201 ggcggcatat ggtgaatttg tctgccgcgc ttatattaag tactgtacca tcttttcctg
     1261 taataagcaa atgaaataag aatgaaaaga ttgagaaaac ttccgatttc cagtataata
     1321 tatccatgcg caaaacaaaa tggcaacagg ccatcaggag gaactatgga tattatacag
     1381 aaaatcaaag aagaacttca ggtagaaaaa tggcaggtgg aagctgcggt gaagctgatc
     1441 gatgagggaa ataccatccc tttcatctcc cgttatcgta aggaggtcac cggatctctg
     1501 aacgacgagc agctgcgtaa tctcgatgag agactgacct atctgagaag tctggaagat
     1561 aaaaaggagc aggtgctgaa gagcatcgag gaacagggaa aacttaccga tgagttaaag
     1621 gagaagattc tggcagctca gacgctggta gtggtagagg atctgtaccg cccctatcgt
     1681 cccaagagaa agacccgtgc cagtattgca aaggagaagg gtctggaacc tctggcagaa
     1741 tatattctgc gccaggaggc aacagaaccg gtgcttaacg aggcagcaaa gtatgtttcc
     1801 gaagaaaaag aagtaaagac tccggaggaa gcattgcagg gagcacagga tattattgcc
     1861 gagatgatct ccgacgatgc ggatcatcgt ctttacatcc gcaatatcac cgtagaggaa
     1921 ggcattgtga ccggtacggc gaaggatgag aaggctcaga gtgtctacga gatgtattac
     1981 aatttcgaag agccggtgaa aaagattgca ggccatagag tattggcatt gaaccgtgga
     2041 gaggcagaaa aagtactgac tgttaaagtc aatgcaccgg aggaacgtat tctgcgctat
     2101 ctggagaaaa agctgatcac aaaggaaaat gaatatacta caccggtaat ctgtgccgca
     2161 gtggaagaca gctacgatcg tttaatagca cccgctattg aaagagagat ccgtaacgat
     2221 ctgacagaaa aggcagagga cggagcgatc aatgtcttcg gtaaaaactt agagcagctg
     2281 ctgctgcagc ctcctatcgc gggcaaagtg gtattaggct gggatccggc attccgtacc
     2341 ggatgtaagc tggcggtagt ggatgctacc ggaaaagtgt tggatacgaa ggtaatctat
     2401 cctaccgcac cgcagaataa ggtggaggag tccaaagctg aactgaagaa actgatcaaa
     2461 aaatataatg tggatttaat ttccgtagga aacggtacag cctccagaga gtccgagcag
     2521 gtaatcgtag aactgttgaa ggaactggat cgtccggtac agtatgtgat cgtcaacgag
     2581 gcaggtgctt ccgtatactc cgcaagtaag ctggcgacag aagaatttcc taattttgac
     2641 gtgggacaga gaagtgctgc atctatcgcc agaagattac aggatcccct ggcagaactg
     2701 gtgaagatcg atcccaagtc catcggtgtc ggccagtatc agcacgacat gaaccagaag
     2761 aaattaagcg atgccttaag cggcgtggtg gaggacagtg taaacaaggt cggtgtggat
     2821 ctgaatactg cgtccgcatc ccttctggaa tatgtctccg gcattaacaa gactattgcg
     2881 aagaatattg tagattacag agaaaataac gggcgttttg taagcagaaa gcagctgtta
     2941 aaggtgccga agctgggacc taaggcatat gagcagtgtg ccggtttctt acggatcccg
     3001 gatggtaaga atcctctgga tgccaccagc gttcatcccg aatcctacga agcagcggag
     3061 cagctgatgg cgaagctggg actgacaatg gaggacatta aggaagccca gaaacaggcg
     3121 gcagctaaga aagcttccga caagagcggg gcacaggctt ccggacagaa gaacggcagt
     3181 aatgcagcca atacagcaaa gaaacgggaa gagcagaaag gtaagaccgt aagagtgcat
     3241 aataccaata ccgctatggg caaggctctg gcagcggcca tgggtggtgt gagctttgat
     3301 aattctgcag aaacttctac aaagaaaaat gcaccggcag tacagaatgc atcaaatgat
     3361 gcaaaagata tttccggact ggaaaagcgt attaagaata agaaactgtt ggcagaagag
     3421 ctgggaatcg gcgagatcac gctgacagat atcctgaagg aactggagaa gccgggcaga
     3481 gatcccagag acgatatgcc gaagccgatc ctgcgcagtg atgttctgga tatgaaggat
     3541 ttaaagcccg gcatgattct gaaaggaacc gtgcgtaatg tcattgactt tggtgtgttc
     3601 gtggatatcg gtgtccatca ggacggactg gtgcacatct cccagatcac cgaccgctat
     3661 atcaagcatc cgctggaagc tgtcagcgtc ggagatattg tggatgtgca ggtattgact
     3721 gtggatgtgg cgaagaaacg tatcggtctg accatgaaga tccagaagca gtaatattat
     3781 gacccgcccc gatatgagca taaaagtggg atgataatcc cacgatatgc gaatagaggg
     3841 tagggtgcta tgtgccagtg gcacatgttt agcacggacc gaagcggagc ggagaagtgg
     3901 gagtgcgtag cacggaacaa tccgcaggca tcaaagtcgg gggcttttga ccccaacatc
     3961 gtattatcat aagaagaaag agggataaca cagcaaaatg ttattcctct ttcttcttat
     4021 gattttataa ccaataacac caaacgatac gaaaagtgaa atagatcttt attatgcctt
     4081 atgacctgtt ttgataatag ggcttaaagg cttgtagatg agcagtgtca caacagagac
     4141 cagcgttccc ttgatgagat tcatgggagc cacacatgcc accacgaagc ttacaatgct
     4201 gccttccttt gccagcggat tgac
//
LOCUS       DAWEJW010000161         4177 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5880_length_4177_cov_60.643620, whole genome shotgun sequence.
ACCESSION   DAWEJW010000161 DAWEJW010000000
VERSION     DAWEJW010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5880_length_4177_cov_60.643620"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1262 a          784 c         1133 g          998 t
ORIGIN      
        1 tctgcgggag gacacctggc agccagcctt ggggtgttct gggatgaaga ttttctggca
       61 gagagccagg gactttccgc gtccgagcaa gagctgctgc gtccggacgg actgctgtta
      121 tgctatccgg tcatcacttc cggagagttt gcccacagag gatcttttga gaatctgtta
      181 ggaagcagac aggaggagct gggagagaag ctgtccctgg agaaacaggt aacaaataaa
      241 gtgccgaaga ccttcatctg gcataccttt gcggatcagt cggtaccggt ggaaaattcc
      301 ctgctgttcg tcagcgctct ccgcagagcc ggagtgccca cagaattcca tatgtatgaa
      361 aagggagcgc acggactggc actggcggat aagctgacag agaccaacga tggcagagcc
      421 gtacaggagg aatgcacctc atggatccgg ctggcgcata cctggctgga gagccttttt
      481 gcaggagacg tggaagacgg actgcacgca cagggcagag aatggtaaga atagcaataa
      541 gagaaaatga aaaacgcaag gagcagtcat ggaacaggca gatacgatca ttcagatccc
      601 tcatttctac ggaagtctga aaggaatgca gaacaaattt gataaatatg caaggcagga
      661 tgctttcaca gggtccacca gagaggaatg ggaaagttgg aaagagactt ccagagagac
      721 attaaaagac cttctgggat ggaaatatat ggagacctgt gatctggacc cccgcgtgga
      781 agaagtcgtg gagctggaga acggcattcg tcgggaaaaa gtgatccttc aggtagagcc
      841 ggaagtgtat atgcctatgt atatactgat cccgccgaag cagagcgaag agaagcagaa
      901 atgttttctt gcccttcctg ggcatcaggg agcaggtaaa ttcagcgtgg caggacgcaa
      961 tgatattccc gcggtaaaac ggatgatcga attctaccat tatgattacg gtatgcagct
     1021 ggcgaagcgt ggttatgtgg caatctgtcc ggactgcaga ggcttcggag aacgcaggga
     1081 tgaggcattg cagaaaggaa cggatgagaa gtccttcctg accagtacct gttttcatct
     1141 ggcacatatg gctgaacctt tgggagagac cgtggcaggt atgtgtacct gggatgcttc
     1201 ccggctgatc gattatgtct acgaacgggg cgaatgggat actgatgatt taggcgtcgt
     1261 gggattttcc ggcggtggta tgcagacgct ttggatctct gctttggatg accgtgtgaa
     1321 aaaatgtatc atcagcggat atctctatgg atatcgtgac gcattgttat tactcaacgg
     1381 taattgcagc tgtaattatg ttccccatct gtgggaacac tttgatatgg gagatattgc
     1441 atcgctcatt gcgccgagac cgctggcggt tcagtcctgc cgggatgacc atctgaacgg
     1501 tcccagagga ctgatgaatg tgacggagca gatggatatt gtgcggaagg cttacagcct
     1561 gtacggaaaa gaggaatacc cgatccacga tgtcagagag ggagaccact gctggcatga
     1621 agaggtattg gatatcgccc tgccaacact gtaagacatt tataacccct gttagtagtt
     1681 aaaaatatgg aggaacgtat ggaatatatc gttctggatc tggaatggaa tcagagtaac
     1741 accggcaaag aggatgctgt ggagaagctg ccttttgaaa tcatagagat cggtgccatc
     1801 aaactgaata atgaacgtgt catggtcagc gaattcaatg aactgatcaa gccacatgtc
     1861 taccatgaga tgcacaagat caccagcaaa ctgatccata ttcagatgca ggagctggaa
     1921 cgtgggagac cattcccgga ggtcggagga gattttgtca gatggtgcgg acaggaggag
     1981 tatctgttct gtacctgggg cacgctggat ctgacggagt tacagcggaa tatggcatat
     2041 tatgagatgc cgcttctggc accagggccg ctgccctatc tggatgtgca gaagctgttc
     2101 gccattgcct atgaggagcg caagatccgc agaaatctgg aatatgccat tgattttctg
     2161 catgttgaga aggatattcc cttccaccgg gcgttcagtg atgcctacta tacggcaaag
     2221 atcctgatcc ggatcctgga ggagcacccg gaggtagtgg tgaatctgtc ctacgatacc
     2281 ttctgtcctc cgaaggaccg tggggatgag gtgaaagcgc agtttgatac ctatgtaaaa
     2341 tatatttcca gagaatttaa ggacaagaca gaagccttcg cagataagga agtcgtgtcc
     2401 agcaaatgtt atctctgcca tcgtaatctg cgcaagaaga tcaaatggtt cagcgctaac
     2461 ggcaggcatt attactgcgt ggcttattgc gagaagcacg gctatctcaa gggcaagatc
     2521 cgggtacgca aggcatatga cggcggcgta tatatcgtca agaccacgaa gctgattcct
     2581 aaggaagagg cagaagctat tgcggcccgc agagatcacg ccagagaagt gcgcaagaga
     2641 cataagcaca gcaaggcggg aaataccggg gaatagggac agaatatgca aattacattt
     2701 tagagggagg atggcagcgg ctacgaggca agatttacga cctgcggaat ctgtaccctt
     2761 atgaaggaac tgggacttac tgagctgatc cctgccatgt gccagtttga ctacaccatg
     2821 agcgaggcgg gcggtgtcac caattttgtc agggaataca ccctggcttc cggcggatct
     2881 tattgcgact gcgggtataa gaaaaagttt aagtaatttg aaataatcat tgtattttta
     2941 caacatatag aatatactaa aaataacaga acccaacacg cctctcaacg atgcggacca
     3001 cgttgggtct tttaatttta agggggaaaa tatatggggg aactaaagca acatccatta
     3061 ccgatgacaa tagacgaaca aatagaaaac ttgaaatcac ttgacttaat tattgagaat
     3121 gaggaatatg ccaagaagat attaaatgac atatcctatt tccgattaat taaggcgtat
     3181 agtttaggat ttaaacctaa aaatgggaaa tatgaagaag gcgtaacatt tgagcagatt
     3241 gtagagttat atctgttcaa tgctaatttc agacaagtga catttgctga gatagaaaaa
     3301 atagaagtaa atgtcagatg cagaattgca aattattttg cggaagttta tggagttctt
     3361 ggatatatgg aaccccaaaa ttttgtggac gaagaatatc atcgggcatt tatggctgat
     3421 atcgaagagg aggttcggag aaactcaaag gcgccgtttg tgagaaattt caaaactaat
     3481 tatgcaggtg gaaatttgcc tatatatgcg ttggtggagg tatttagttt tggcactctt
     3541 tcaaaatttt acaaaaacat gaaaaatgct gacaaaaagg cagttgccaa gagctttgga
     3601 attggatata catatctgga aagttggttg gagagcattt cttatgttcg caatgtttgt
     3661 gcgcattatg ggaggttgta taatgcaaaa ctttcaaaga cgcctattct ttacaaagaa
     3721 tatacacaag ccggtatagg aaacaataga atgtttggtg tattattgtg tatgaaacag
     3781 attttaaaga atgacaagca ttggaatctt tatgtagatc aaatagaatt gctaatagat
     3841 aaatatgaaa aagtagatgt gaaaacgatg ggttttccgg atgactggaa gaaattgttg
     3901 gaacagaaat aggtgtaata gaagagtata caatggaaaa cacagtagat accgggattc
     3961 tacttccggg aggtgtactt atttctgaaa agagaatagt atgatctcag acacaggagg
     4021 taagcacatg gatctcgtca agataggaaa atatattgca ggcaaaagaa aagacttagg
     4081 tatgacgcag aagcagctcg ccgaaaaatt gggtatgagt gacaaatccg tatccaagtg
     4141 ggagcgggga gtttcccata gttaaagata ccacacc
//
LOCUS       DAWEJW010000162         4142 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5923_length_4142_cov_43.015170, whole genome shotgun sequence.
ACCESSION   DAWEJW010000162 DAWEJW010000000
VERSION     DAWEJW010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5923_length_4142_cov_43.015170"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1174 a          781 c         1154 g         1033 t
ORIGIN      
        1 acagaggccg atccggcaga gacggtggtg aaggccgcag tttcggcaga ggcgacggaa
       61 gacggaagtt tggccgtgaa gacggggacc gcagaagatt cggtcgcggt gacagagacc
      121 gcagggacga cgacaatacc gtttccggcg agaacagaag acaccgcaga gacgagaacc
      181 gcgaggacgg aagaaaatgg ggcggccgcg acaaaaaggc agcggaccgt aaatactccg
      241 gggacagagc ggagagagaa gtaaaaaaga gaaagaagcg ggaagagccg ggcatcggca
      301 acagcttccc caagagaaaa aaatcttaag tagatgagga attttatgta tttgccgcaa
      361 aagccgcagt tgtgcttttg cggcaaatcg tgtatagtta gggaagaatg cataggcact
      421 aacagaaagg tctgcggtat gaaaacactg aaaaaacaga taccatatat tttgctggga
      481 gcgacgcttt tgctcctgct tggcttgaat atcatcagcc aggatcactg gctggattcc
      541 gatatggcgg cagagatgat cttttccagg atccttgcgg gggagcatca gatgatttcg
      601 acgacgaact ggtattattc cacggagttc cgtgtgctgt atacacagct gatcatggga
      661 ccgctgttcc ggatctgcaa caactggcat gtgatccgca cgatcacgaa tctggtgttt
      721 tacgggctga tgctggcttc ctattattat tttatgaagc cgttaaaagt atcgcgcggg
      781 cttacggtgt taagctcctg tctgttgctt ctgccttttt ccgagaccat gatgacacac
      841 atgcaaatgg gcaataccta catgtcccat gtgattctgg tgttgtggtt cttcggtatg
      901 tacctgcggc tgtgcagtgg agaatacagt acaaaacgca agatcagcct gtggatcttc
      961 tatgtgctgc tggctattgt ctgtggaatg tccggcgtac gttacctgct ggcattacag
     1021 tgcccgttgg tactgacttc ttttttctat ttgttgggtg gagaggagtt ccagagtttc
     1081 cggggagaga tgacaaaggc gcattttcgt gccttgctgc ctacgacccg gatgaaatat
     1141 tttctctaca gtgtagtggg agcgtttttc gcagtagcgg gctacggcat taatgtggta
     1201 tggatcagcc ataaatatgt gttccagacc tacggtgcca cgaatttcat cgctctgtac
     1261 cacggggtgc tgtttgaccg gatccagaat gcagcaggct gtctgttaat gctttttggt
     1321 tatattcccg acaagggctt tttatccctg cgtggtattg tgaccatggc ggctttcgca
     1381 atgctgggca tctacggata tgttacggtg aagagcggaa agacggagca gagtacagga
     1441 tttcgcagcc tgatcacact tttcctgaaa gtcagctttg tactgaatct gtttgtgttt
     1501 attttcacca ccagtaccat ggtgccacgg tattatatta cgatttttat ttttgcactg
     1561 ccggtattgt gcttttatct ggaagaggag caaatgccat ttgaccgtct ggcggtgacg
     1621 gcgctgctta caatctgtct gatccttggt acgggtaaaa cggtgatgtc tttcctgacg
     1681 gtggacaaga acgaaacgaa gcgtcccgtg gcgcagttcc ttgcggaaaa cgggtatgat
     1741 tttggctttg ctacttataa taatgccaat atcattacag agcttaccaa tggcgaagtg
     1801 gagatcggca acatcggaga tcccgaacat ctggaatatt tcaagtggtc ttctcccatg
     1861 aaatattatg aggaagggta tcataccggg gagacattct tgcttctgac gtcggaggaa
     1921 tgtgcggaat atgcagaggc tccggcatta aagcagggcg aaaaggtata tgaggacggc
     1981 atttataccg tctatctatt cgacagcacg gaggaactga tgaactgtgc tgtggcgaga
     2041 caataatgat agagatttag tatattgcgg acggcattgg cacccggcac tggccgcaac
     2101 gggacagtcg gtactttttg aaaaaggata acgtctgcgg aaaggttgta aatatgagaa
     2161 tcggaatggg atatgacgta caccgtctgg tggagggaag agacctgatc atcggtggcg
     2221 tgaaaatacc ttatgaaaag gggctcctgg gacattcgga tgcggatgtc ctgctccatg
     2281 ccatcatgga tgcgctgttg ggagcagcgg cgctggggga tatcgggaaa catttcccgg
     2341 atacagatcc ggcatacaag ggtatttctt ccataaaatt gctggaaaaa gtgggcgagc
     2401 tgctggaaga gaatcatgtg tttatcgaaa atatcgatgc gacgatcatt gcacaggcac
     2461 cgaagatgcg cccgcatatc gataccatgc gggaaaatat cgcgaaagcc ctgctgatcg
     2521 atgtgggaca ggtcaatgtg aaggctacca cggaggaagg actgggattc accggcagcg
     2581 gagagggaat ctcctctcag gcaatctgtc tggtgactac gccgtttgat cttacggcac
     2641 aggcgatgca gggcggctgc ggaagctgca gcggatgccc gaagagctga taattttgga
     2701 aaataagaac ttgacaaatg ggtcgatagg atatagactt tgattagaac aaggtctgta
     2761 tactatcgac cttttataat gtaatgagtg caaaaaagcc aataggattg cataattggc
     2821 aagggctgga ttaagaggga tacaaggagg aatgggaatg tatcagatga cggagacaga
     2881 atataaattt gcagagctga tctgggagga ggaacccatc ggttccggag aactggtaaa
     2941 acagtgcgcg gagaagttcg gctggaagaa atccactacc tatacgttta ttaaaaagct
     3001 ttgtgagaac ggtattttca aaaatgagaa tgccatcgta tcttccgtat tgaataagga
     3061 ggaataccac agagcccagg gggaggcttt cgtggagaag gcttacgggg gttcgctgcc
     3121 gaagatgatc gccgcattta tccagagcaa gaagctcagt gcagcgcaga tcgctgagat
     3181 cgaagctatg atcgaggatt ataaggggta ggggttgaca aaatactatt gtgatattaa
     3241 aggttctatg ctaagataaa ttgcatagga cctttttcta ttatcagatc tattttctat
     3301 tagaatccaa tgtcggagaa attttccaat ccattgaaat taagtagaaa atatactttt
     3361 aacttcattt ttgcaactaa aaacagttgc aaaaaacagg cagaaaaaat ataatataat
     3421 taacaagggg tacaaatggg acaaaaggaa aaactaattg aacagttaaa aagcagaccg
     3481 aagacattta cctttcatga tgcggaagtt ttattggatt atttggaatt tacgaaatcc
     3541 aataagggga aaaccagtgg ttccagagta atgtttatca gtgaggagca cgggagtata
     3601 ttactacata agccgcatcc gcaaaaggaa ttgaaagcat accaggtcag acaattacgt
     3661 gatctgttgg aacaggaggg attgatttga aaaatacgat ggaatataaa gggtatgtcg
     3721 gcagcgtaga attttccgag gaggatggga tttttttcgg aaaggtaatg gggattcgtt
     3781 ccctgatctc ttatgaggga accgatgcca ggagtctggt agaggatttt catggggcgg
     3841 tagatgatta tctgcaactg tgtgaaggac aggggaaagc tccggaaaaa gcatacaagg
     3901 gaagctttaa tatccggatc gcacctgaga ctcataagca gcttgtgatc catgcaactg
     3961 catgccagat gtcactgaat gaatatgtga gggaaacgtt ggaaaaagca gtgatgtaga
     4021 aaactccggg gttgacaaaa cccgtatttt tgcatatctt atttagtagg aatgatcgta
     4081 tattcagtaa aggcatggag caggaaaagt acttttcggt gggacgccag agagaagctg
     4141 tt
//
LOCUS       DAWEJW010000163         4090 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_5994_length_4090_cov_72.511276, whole genome shotgun sequence.
ACCESSION   DAWEJW010000163 DAWEJW010000000
VERSION     DAWEJW010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5994_length_4090_cov_72.511276"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          947 a         1092 c          874 g         1177 t
ORIGIN      
        1 catttgacta atgctctttg ctaattactc tattttatag gtgtcgggtt caaaagttct
       61 cgacatctat aatgttccat gggagaacag tcctaccggt ccctgaggag gatccttagg
      121 atccttcgac tcctcggcag catcgctttt ctgctctgtg gattcctgtt catgcttctc
      181 ctgtgcggaa gtctcttctt ttgcggattc tgcaggctgc tttgcggtat cctgcgccgg
      241 tgcagtctgc tcctttgctt cctgcaggct gtcttcgaaa gtcaggggtt ggaaagttga
      301 atcggtagaa gtcatgacct gttccccaat ggtcacatcc acgctgttgc cctttactgc
      361 actgcccgca gcatttgttt ctgtcacatc ttttttctct tcctctggat cgggcagtcc
      421 cttctcccga cggaagatct ccatgaattc cttaccggtg atggtctcac gctcgatcag
      481 gaacgctgcc agcttatcca tcagttctct gttctcgcga agcatctgca atgcctgatc
      541 gtagctctcc ttgatcatgg caacgacttc ttcgtcaatg gctgctgccg tttcatcact
      601 acagttcagc gcggttcttc cctccagata ttggctctct acggtagcca gacccacaag
      661 accgaaacgt ttgctcatac cgtagcgggt gatcatgttg cgggcaatgg atgtagcctt
      721 ctcaatatca ttggctgcac cggtggtcac agtgtcgaat actacttcct cagcggcacg
      781 tccacccacc agacctacca gcatatcccg aagttctgcc tcggtattga ggtatttttc
      841 ctcctccggc acctgcatca catagcccaa cgcacccatg gtacggggga caatggtaat
      901 tttctgtacc ggctcggagt ttttctgtaa agcgctgatc agtgcatgtc ccacttcatg
      961 ataggatacg atgcgacgtt cctctttgct catgatgcgg tctttctttt ccttacctac
     1021 cagcacctgt tccacagccg cgaacagatc cttctggctt acaaattctc tgccttcctt
     1081 gacagcattg atagctgcct cgttgatcat attggccaga tcggaaccga ccgctccgct
     1141 ggtagccagt gcaatcgcat ccagatctac ggtctcgtcc atcttcacat ccttggaatg
     1201 tactttcagg atttccacac ggcccttgag atccggcttg tctacgatga tacggcggtc
     1261 aaaacgtccg ggacgtaaga gcgccttgtc caggatctcc ggacgattcg ttgcccccag
     1321 gatcaggata cctttggaac tgtcgaagcc atccatttcg gataataact gattcagcgt
     1381 ctgctctctc tcttcattgc ctccaccgta cttggaatca cggcttcgtc cgatggcatc
     1441 gatctcatca ataaatacga tacagggcgc gttcttggtc gcttccttga acaggtcacg
     1501 gacacggctg gcgcccacac ctacatacag ctcaataaaa tcagaacccg tcagagagaa
     1561 gaacggtaca tgtgcttctc ctgccactgc cttggccagc aaagtcttac cggttccggg
     1621 agggcccacc agcaatgctc ccttgggcag ttttgcaccg atcttggaat atttctgtgg
     1681 attatgcagg aagtccacga cttccaccag agattccttt gcttcgtctt caccggccac
     1741 atccttgaat gtaacgccgg tctccttctg tacatatacc ttggcattgc tcttgcccac
     1801 gcccatgggt ccgccgcttc cgcccattct gcggaacaga aaacctgcca cgatccagat
     1861 cagtaccacc ggcaatacca cggttaccag aatctccatc aggaaattgg aagtattgct
     1921 ggtcacgcga ttcccctgca catcatgctc ctgcaatctg gctgtcaggg tatccagatc
     1981 ctccatcagc attgtataat atacagtctg ggactgtgtc accttctggg taattccata
     2041 gaagctgaca taactgcccg gtgccggggt ggctgcagct tcctgcttca gctgtaccgt
     2101 caccatgccg ctttccttat ttacctccac cgcagcaacc ttgtccgctt ccagatcctt
     2161 caggaaatcg gtataagata cttccgttcc ggtattggtg ccgaagaacg cattatacag
     2221 catcagtaca acgatcactg tcatcagtcc cgacaggatc agcatcagga tgctggggcg
     2281 cttcggtgaa ccattgccgt tgcctccgtt actgttatta ccgttcccgc cattgttacc
     2341 gctgccggga gctccgttgc cattgcctcc gttgccgggg ccatagccgt tattatatga
     2401 ctcaattaat tttctcatgt catctccatt ttctatttta tacgctattg cacttccgtt
     2461 atttcaactg cacagttgaa atctgagcaa tcatatctat tatagagata acctgtggat
     2521 aaatcaatag ctcaaatatg aaaaaactct gaactattcc cggaaataac gaatatccgg
     2581 aaaccatatt tttctacagt ttccgggcat tcgccagcaa caaacaactc acaacaacca
     2641 attatatact aacgcccttc aaacataacg ttgatctccg attgatacaa agtccaggta
     2701 ttgatcaggg cattcatacc ttcgttgggt gtctggatct gcagtgcagc gtatttctgt
     2761 ttccagtagg ctgccagatc ggtgtagact gcgtccactc ttttcagatc gctgtatttt
     2821 acccggatcc gtctgccctc ttcacgattg ccctcgccta gcatgatcac aaaacgggct
     2881 tcttcgccgg actgtaattt gaagttcttc tgcaagctgc cgcagtgatt gccaccaagt
     2941 tctgtggaac cttccagagt tcctctctcc aggcctgccg gatgatcttc ggtaccatag
     3001 actcccagga atttctctct gacgcagtca tagccgtccg gtgtgaagtt ggcggtaaga
     3061 tactgatacc ctttttcttc gtagaacaag tcctcctcga tgatgccgtc ttcataggaa
     3121 gaaccggcac aatacagact catctgaaaa ttctggttgt ctatcataat gtgatggaac
     3181 gaaaattcca tgtaagtaaa taaggaaaga tttctgactg catcggtggt atttttcacc
     3241 tatacatccc acaacagtac attttccccg cggggaataa ccatggtctg tgaggatgcc
     3301 agtccgtcat attcgctctc gtagacggtg taagacagtc cgtggcggca acggtatttt
     3361 gccttggata catccttggc taccggctga caggagatgg aatggtaatc cttcttttca
     3421 ttatccctga tatatacata gaatcccggg cggtccatgg gtaccccatt cccacggaac
     3481 cggctgatcc tgtgatactc cggtgttttg taaaacagat aacctcccgc cgtatggttg
     3541 atgaccgccg ccatgtcctc gacacccaga tagttcgtcc aggaacaggg cagagccacg
     3601 ttgtcgatga cgtattctct tttttcatta tcgaaatagc cgtattgcaa aacgcgtccc
     3661 tcccgtatat agttttttgt tttttcagaa cttctttgtt ctgtatttat tgtaatttca
     3721 tgtaactatt cagagccatg caaaactgcg ttctgcgaat gcttgcattc aggcagataa
     3781 gcagttttat atggcgaagt aaccacatga gcaaaaggaa gctacgaagt agcggttttg
     3841 cgaatggggt tctgaatagt tacaatttca ttataaaaaa ggatctcttt gttgaaaatt
     3901 gctcattatg tgattatttg cttaaaattg ttagatataa taatgatttc caaaaaaaat
     3961 tttactttcc agtagatttt tgggaaacac taaactataa tagaacatca tgggatttta
     4021 gttgcaaaga ttcgcagaga aaggtgtcag caatgtacaa gataggtttt gacaatgaca
     4081 aatacctcag
//
LOCUS       DAWEJW010000164         4074 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_6030_length_4074_cov_65.565066, whole genome shotgun sequence.
ACCESSION   DAWEJW010000164 DAWEJW010000000
VERSION     DAWEJW010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6030_length_4074_cov_65.565066"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1200 a          820 c         1064 g          990 t
ORIGIN      
        1 agatattgcc ggacttccgg tggtggcttt gggacgcccc atgggtacca acggttccgt
       61 gggctatggt atcattacct cttccgccag tgagtcggcg gtatcggata ccacttaccg
      121 gattctgcag accaatattg taggaagtca gaatgcaggc ggtgtattgt ttaatctcca
      181 gggacaggtc atcggtatca ttacaaatgg caagtctgcc acggatatga aaaatatggt
      241 atgtgcttac ggcattacgg aattaaagcg ccatattgag aaaatgtcaa acggggaaaa
      301 atttgcatat ctgggtctga agggtgtggg tgtcaccgaa gaagccaaca gtgaactggg
      361 agttccctac ggtgcttata taacagaggt agaaatggac tctcctgcaa tgctggcggg
      421 aatccagcag ggagatgtca tcacaggatt cggtgaacgt gtgatcgtca gctttgacga
      481 gtatacgaac tctctgctca gtatgaaacc gggagatagt gtagaactga cgattatgcg
      541 gcagtcacag caggaatata aagagatgaa attcgacatc acattaacgg tgttgaaata
      601 gattgacaga aaggtttgga caacaatatg aaattcatta aagagttaaa agaaggcgac
      661 agagtatttg atatttatct gtgcaagcat aagcaggagg ctgtaaccaa aaacggtaaa
      721 ccctatgaaa gcgtgattct gcaggataag accggaacca ttgatgccaa ggtatgggag
      781 ccgaacaatc cgggcatcgg agattatgat gatctggatt acatagaagt atacggtgat
      841 gtgacgaatt tccagggaca gttacagatc agcgtgaagc ggatccgtgt ctgccgcgag
      901 ggagaataca atccttccga ttatctgccg gtgagttcca agggcatcga cacgatgtat
      961 aatgagttaa agactttgat cggaagcatt aagaatacct atctacatca gctgttgcag
     1021 gatctgttta tcgacgatgc tgatttcgca aagaaattct gcaactcttc tgcagccaag
     1081 acagtgcatc atggtttcgt gggcggattg ctggagcata ccttaggagt taccaagttg
     1141 tgtgactact actgctctgc ttatcctatt ttacagagag atctgcttct gacggcggct
     1201 atgtgccatg atatcggaaa ggtaaaagag atttccgctt ttccggtaaa cgattataca
     1261 gatgacggac agttattggg acatatcgtg atgggcgcac agatggttgg ggaacgtgcc
     1321 gctaagatcg acggcttccc gcacaatctc ctggcagaat tacagcactg cattctggca
     1381 catcatggta aattggaata cggatctccc aaggtgccgg cgctgatcga ggcagtggct
     1441 ctgaattatg cggatgatac cgatgccaag atggagacct ttaaggaaat tctggaaaac
     1501 aacagtgaaa acagtggctg gctgggttac aacagattgt ttgaatctaa tctgcgggct
     1561 accagaatgg agtaaatgac aggataaata ctataaattc tgtgaacaga caggaaagag
     1621 aggaataata tggagatcag tacatgtgta aaatatgtgg gtgtaaacga ccatcaggtg
     1681 gatttgttcg agggacagta taaggttccg aatggaatgt cttacaattc ctatgtgatc
     1741 ctggatgaga aaatcgccgt tatggataca gtggatggat tttttaccga agaatggctg
     1801 aacaatgtag aacaggtact ttccggaaaa acaccggatt atcttgtgat tcaacatatg
     1861 gagccggatc attccgccag cattcccgct ttcttaaaaa aatatcccaa aaccacagtg
     1921 gtatccacag cgaagggatt ccagttcatg gaacagtttt tcccggagtt tttcgccgtg
     1981 cagggattac ctgcggaaca acacatcgtt gcggcgaatg atggtgtcct ggagcttgga
     2041 cagcacagcc tgcattttgt ctatgctccc atggtgcatt ggccggaggt tatgatgact
     2101 tatgaagcaa ccgagaaaat actgtttgcg gcagatggtt tcggtaaatt cggtgctttg
     2161 gacgtagagg aagaatgggc gaatgaagct cgccgctact atatcggtat tgtcggaaaa
     2221 tatggtcccc aggtacaggc ggttctgaaa aaagcggcag gactggacat tcagaccatc
     2281 tgctctcttc acggtccggt acttaaggag aatctgggat tctatctgga aaaatatgat
     2341 aagtggtctt cctatcagcc ggaagaatcc ggagtggtaa ttgcttatgc ttctgtctat
     2401 ggcaatacca gaaatgctgc ggagtatctg gcggatgttt tacaggaaaa aggacagaaa
     2461 acagtactgt atgacctggc aagatgcgat aaagcaaaag ctgttgccga tgctttccgt
     2521 tatgacagac tggtgctggc gggtattact tataatggag acctcttccc ctgcatgcgc
     2581 agttttatcg aaggtctgac cgagcgtaat taccagaatc gtaaggtggc aatcattgag
     2641 aacggcacct gggcacccat ggcaggcaaa ctgatccttg gaatgtttga aaaaagcaag
     2701 aatctgacat ttacggagac tacagttagc atcaagtctg ccatgaatga gcagaacaag
     2761 ggcgagatcg gaaagctggc tgaggagctt tcctgagaaa gtttttcaca cgtaaatttg
     2821 tgcctgcaca gtgtaccaaa gtacgaggga ctgatgtaag caatactgga agtacgcggt
     2881 actgaagtac cggaaaattt gtaggctacg gaaaggcata aggatattta tggaatataa
     2941 gaaaaacgat cgtgtaacgc tgaccataga ggatatggga agtgacggag aaggaatcgg
     3001 taaagtagac ggttttaccc tctttatcaa agatgccgtt atcggcgatc aggtggaagc
     3061 caagatcata aaaagtaaaa agcactacgc ttatgccaga ttagagaagg tgttacagcc
     3121 ttctcctttt cgtgtagaac cgaaatgtgc ataccacaga cagtgcggcg gctgccagtt
     3181 gcaggcaatc tcctactctg agcagctgca tttcaaggaa cagaagatcc gcaacaatct
     3241 gatccggatc ggtgggttcg atgcggaata cattgatgaa cgcatgcagc ctattgtagg
     3301 catggaggaa cccttccact atcgtaataa agcgcagtac cccataggaa ctgatagaga
     3361 cggtaatgtt atcaccggtt tctatgccgg acgcacccat accatcattg ccaatacaga
     3421 ctgcgccctt ggtgcttctg agaatcagca gatcctcgaa accatactct cctatatgcg
     3481 gaaaaataaa gtaactgcct atgacgaagt cactggcaag ggtctggtac gtcatgtatt
     3541 gatcaggaaa ggcttcacca gtggacagtt gatggtatgt ctggtcatta ataagaaaat
     3601 gacaaaaatg tcatacccta ttgccggagt acagaagaat acgaatgagt ttttaccaaa
     3661 tcagggagaa ttactggctg cactggcaaa aatccaaggt atgacaagtg tgtcagtcag
     3721 tatcaatgcc gagaaaacca atgtgatcat gggtaccatg atccataatc tctggggtga
     3781 gagcaccatt gaggatacga tccacgtaag ggacatgcag aaagaaaatt acccttatac
     3841 cggagatgcc ctgaccttca agatatctcc cctctccttc tatcaggtga atcccgtaca
     3901 gacagagaag ctttacagtc tggctctgga atatgcggga ctgaccggaa aagagacggt
     3961 gtgggatctc tactgcggca tcggaaccat ttccctcttc ctggcgggca aagccaaaaa
     4021 ggtgtgtggc gtggagatca tcccccaggc catcgacgat gccagagaga atgc
//
LOCUS       DAWEJW010000165         3995 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_6169_length_3995_cov_65.938071, whole genome shotgun sequence.
ACCESSION   DAWEJW010000165 DAWEJW010000000
VERSION     DAWEJW010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6169_length_3995_cov_65.938071"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1146 a          909 c         1065 g          875 t
ORIGIN      
        1 tcgaaaggag attatgtatc atggcaaaca gaattatgct caaccagacc tcttatcacg
       61 gtgcaggagc aattgcagaa atcgtaaccg aagcaaaggc tcatgctttt cagaaggcat
      121 tcgtatgttc cgatccggat ctgatcaagt tcggtgtgac cggaaaagta accggactgc
      181 ttgacaagga aggtcttgct tatgaagttt attccgatat taaggctaat cctactattg
      241 acaatgtgaa aaacggtgtg gctgctttca aggcatccgg tgcagattat atcatcgcca
      301 tcggtggtgg ttcttccatg gatacctcca aagccatcgg tatcatcatt gccaaccctg
      361 aattcgaaga tgtacgcagc ctggaaggcg tagcacctac caagaaaccc tgtgtaccca
      421 tcatcgcagt acccaccaca gccggtaccg cagctgaggt tacgatcaac tacgttatca
      481 ccgatgtaga acgcaagcgt aaattcgtct gtgtagatcc tcatgacatg cccatcatcg
      541 ctgtggtaga tcccgagatg atgtcttcta tgcccaaggg actgaccgct tctaccggta
      601 tggatgctct gacccacgct atcgaaggtt acaccaccaa ggcagcctgg gagatgaccg
      661 atatgttcca tctgaaagcc atcgagatca tttccaagtc cctgcgtggc gcggtagcta
      721 atactaagga aggccgcgaa ggcatggctc tgggtcagta cattgccggt atgggcttct
      781 ccaacgtagg tcttggtatt gcccattcca tggcacatac tctgggtgct gtctatgata
      841 cccctcatgg cgtagcctgc gcaatgatgc tccccatcgt tatggagtac aatgctgact
      901 gcaccggtga aaagtatcgt gagatcgctc gtgctatggg agtggaaggt gtagaccaga
      961 tggatcaggc aacctatcgt aaggctgctg tagatgccgt tcgcaagctg tccgaggatg
     1021 taggaattcc ttccaagctg gaagcactga aggaagagga tcttcctttc ctggcagaat
     1081 ccgctcatgc agatgcctgc gcacccggca accctaagga tgcaagtgta gaggatctga
     1141 ccgcattgtt ccgcaagtta atgtaatcat taaatttgct actgtttttc cggaaaataa
     1201 tatggtatgc taagaaacga gcaggcttgc gagagcagac ctgctcgttt ttaaaggaat
     1261 atgaacgaaa tcaacaggac aaaggaaaca ataatgggca atacaaaaca taccaaagag
     1321 cagatccgtg aaaggatcca acagaaaaag ctcctctttg acggagcttt cggcacttat
     1381 tacggaggaa aatacgacac gaggcagctg ccggagctgg cgaatctgga ggctccggag
     1441 cgggtaaagg agatccacag agagtatctg gaagcaggag cacagatttt acgaaccaat
     1501 acctttgctg ctaacagctt ctgcatggat ataccccggg aacagatcgg agagacactg
     1561 cgcagtgggc ttcgactggc aagggaagca gtggcagagt ggagagagac caccggagag
     1621 acgaaagaga tctacatagc ggcggacatt ggccagattc caggggaggc catggcacag
     1681 aaagatgctt taagcagaga atatgaagaa atttgtaata tttttctgga agagggagcg
     1741 gacttttttg tctttgaaac tttttccgag atggaagagc tcctgcctgc gatcaaaatg
     1801 atcggggaac aggcatttat tacggtacaa ttctctgtaa accagttcgg ctacagcaat
     1861 gcaggattaa gtgccagaag gcttttacag agagcgggtg aggcaaagga gatcgatgcc
     1921 gtgggcttta actgcggtgt gggtccgtcc catatgcacc ggatcctgca gatactggaa
     1981 aaaccggaag gaaaacttct cacagccctt cccaatgcgg ggtatcctca gatggtcacc
     2041 ggccgtatgc tcttcaccgg ggataacaaa gactattttg tggacagaat gcagcagatg
     2101 actgcccttg gagtggatat ggcaggcggc tgctgcggta cgacaccgga atatatcgcg
     2161 gagcttgccg gaaagctgga tctgacccag tacccacagg caaaaaaaga cacagaaccg
     2221 gagaaaaagc aggcaccgaa ggaagaccgt tctttttatc ataccaaaga ggcggagggc
     2281 aggaaccgaa agctgatcgc cgtggaactg gcacctccca tgggaatcga tgacgaaaaa
     2341 ctgatggatg cggcacatct gctacagaag agcggagtgg atgtgctgac tttcccggat
     2401 tctccttcgg gacgtaccag agcagattcc attctcatgg cggagaaggt ggcaagagag
     2461 accggtatgt gtgtcatgcc ccatatctgt tgccgggata aaaatgccat tgccatgcgt
     2521 tcccagctgt tgggagccta tattaacggt atccagaatt tcctggtgat tacgggagac
     2581 cctattcctt ccatggtgcg gaccacggtg aagagtgtgt tcaattttga ttctgtggga
     2641 ctaatgcaga tcctggcaga tatgaacgag gagcagttca cacaggcacc ggccagctac
     2701 ggcggagcta tcaatcaggg cagacgtaat cttgaggtgg agatcggcag agtgaagaag
     2761 aaaatggcgg cgggagccac cttttttctg acacagccca tttccacaca ggagagtgcc
     2821 gacagagtgc gccggatcaa agaggagacc ggggcaagga ttctctgcgg gatcatgcct
     2881 ttcgtgagtc ttaaaaatgc cacctttatg aagaatgaga tggcaggcat cgacgtgacg
     2941 gacgaagtac tggcaagata ccgggcggac atgaccagag aagagggaga gcaggccggt
     3001 gtgcagctgg caaaggaagt aatcaccatg acggaggatt ttgcggacgg ctattatttc
     3061 tctttcccct tcaaccgggt ggccatgctg gaaaaaatac tcaattaatg gttttttttc
     3121 aaaaaacagt taacaaacct tggaaaaatc tgaaataagt agtatactgt aactgatata
     3181 aaaggactaa tggctgaaag gccgattcaa aacagttgac acggaggtga ctgtgatgag
     3241 tattaatatt caggcaaaga ccaattattc atttctgttt tccggtttga gctctacggc
     3301 atccaatgcg gcaggcagca actggctggc agattatgca agtattaaga acggcagcta
     3361 tgccaagctg atgaaggctt attacggcaa ggagaacacc gcatccaaaa ccgcagcttc
     3421 cgacataacg aagaaagaca ccacgacgca gaccgcgaag aaagcactgg ccaaggtaga
     3481 gaccgccact gatgctttga aggaatccgc agatacatta ctggctaccg gtaaaaatga
     3541 tctgttcgca caaaaggata tcaccaccaa ggatgagaat ggtatagaga ccacgaccaa
     3601 gggctatgat accagtgcca tctacaatgc ggtgaacagc tttgtgaaga attacaacag
     3661 cgtgatggct gctgtggatg atgtgagcga taccacagtg aacaaccgca cagaatcact
     3721 ggggaatacc accattgcta acagtaagca gctggcgaag atcggtatta ccatgaaaaa
     3781 tgacggcacc ctgtctctgg acaaggatac ctttatgaaa gccgatatga atactgtgaa
     3841 gaacctgttc caggggaacg gttcctacgg ttaccgtgta tccgcacaga gttccatgat
     3901 caattttgca gcagaccatg cttccaccag aagcagtctg tacaccggaa cagcaggata
     3961 taccggcact tacaatgcag gcaatctgtt cagtt
//
LOCUS       DAWEJW010000166         3858 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_6425_length_3858_cov_70.907704, whole genome shotgun sequence.
ACCESSION   DAWEJW010000166 DAWEJW010000000
VERSION     DAWEJW010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6425_length_3858_cov_70.907704"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1000 a          807 c         1031 g         1020 t
ORIGIN      
        1 atatatgatc tgatcaatga gcagaagaaa aagggcgttg ccgtaatctt cgtcggtgag
       61 gatctggatg tgctgatgga gatgtccgac cgtatcatgg tgctgtgcgg cggagaagtc
      121 agcggtatcc tcgatgcgag aaagacagat aaaaatgagg tcggtatgct gatgaccaga
      181 gtaggaggca aagaacatga aggagaagaa taaagagcct ttgtttcata tcgtaaaaag
      241 agatgctctc ccctggtata aatctctggg tatccgtttc gtagcgattc tgttggcgtt
      301 gatcctgtgc ggtatcatta cgacgatcac cacgggaatc aatcctttac aggtatatca
      361 gtctattatt ctgggagctt tcggatccgt ccgtaagacc tgggttactt ttcagaatat
      421 tgcgatcctg ttgttgattg cactggcact gactccggca ttcaagatga aattctggaa
      481 tattggcggt gagggacagg tcttgatcgg gggtctggca tccgcggcct gcatgatctg
      541 cctgggggac aagcttccgg gtgcagttgt gatcctgtgc atgatcgtgg ccagcctggc
      601 agcaggagcc atctggggct tcattcctgc atttttcaag gcaaaatgga ataccaatga
      661 gacactgtct acactgatga tgaactacat agctacccag cttgtggcat tctataccat
      721 tgtatgggag gttcccaagg gatccggtaa gatcggtatc atcaaccaga ataccaacgt
      781 gggctggctg ccccagatct tcggttcaaa gtatctgtta agtattgtag tggcagtagt
      841 gattacaatt ttcatgtatg tatatctcaa ctatagcaag cagggatatg agatctccgt
      901 tgtgggtgag agtgaaaata ccgcaaaata tgtgggtatc aaggtagaaa aggtaatcat
      961 ccgtactatg cttctgtccg gtgcgctgtg cggactggta ggtttgcttc tggtaggcgg
     1021 tatcaaccat actgttacca ccaccattgc aggtggacag ggatttactg ctgtactggt
     1081 atcctggatg tccaagttca atcctctgac catggtgttc tccagcttcc tgatcatttt
     1141 catggatcgt ggtgccagtg agatctctac caattttgga ctgaatcatt cctactccga
     1201 tattctgaca ggtattatcc tgttctttat tatcggctgt gagttcttca ttacctatcg
     1261 cctgcagttc cgtaaaaagg cagaaaagga ggaacagtaa tcatgtggac atttttactt
     1321 gcatttattc cgagagctat tgtacaggga attcctctgt tatacggaag taccggtgag
     1381 atcatcacgg agaaatccgg taacctgaat ctgggaatcc ccggcgttat gtatgtggga
     1441 ggtatctgcg gtgtcatcgg ctccttcttc tatgagagat cggcaggcgg tgcggcaaat
     1501 atgaatggat tcctcgctat tgcgattcct atgctttgtt gtctgttggg ttccctgctg
     1561 atgggactgc tgtactgttt cctgaccgtt accctgcggg cgaaccagaa cgtgaccggt
     1621 ctggccatga ccaccttcgg tgtcggcttc ggtaacttct tcggcggctc cctgatcaag
     1681 ctggtaaaca gcgacgtgcc ttccatcgca ttgtccgcta ccagcagcta tttcagcaaa
     1741 tcccttcctt ttgcggcgaa gctgggatgg ttcggcaagt tgtttttatc ctatggattc
     1801 ctggcttatc tggcagtgat cattgcgttt gcggcatcgt atgtcctgag tcataccaga
     1861 acaggcctgc acttaagagc agtcggtgag ggccccaata ccgcagatgc agccggtatc
     1921 aacgtatccc ggtacaaata catcgctacc tgcgtgggat gtatgatcgc aggtctgggc
     1981 ggactgtact atgtaatgga ttatgcctgc ggcgtctggt ccaacgatgc gttcggtgac
     2041 agaggctggc ttgccattgc gctggtcatc ttcactatct ggagaccgaa tatcagtgta
     2101 tttgcttctt tcctgtttgg tggtctgtat attctgtatc tgtatctgcc caccggtatc
     2161 gagcatatgg agtatcagga gctgtataag atgatccctt atgtcgtgac cttactggta
     2221 ctggtcatca ccagtatgcg gagcaagaga gagaatcagc cccccgcaag tctgggactg
     2281 gcgtacttca gagaagaaag ataataattt tatagaaaga cgattaaaaa cggcgttttc
     2341 cggaaatttc cggagaatgc tgttttttag tgcacatttt ggaaaaaact cacagaaact
     2401 ttaggaaaac ttttgatttt attcggatgg aatttacctg tgtctgttat gctgagatca
     2461 attcagagaa aataaacgga agggagaaaa aagatgaaag tattgatcac aacagactgg
     2521 tacaagccgg ccatcaacgg agttgttact tcggtatgca atctgcggga ggaattacag
     2581 caaagaggac atgaagtgaa gatcctgacc ttgtcccgta cagctcgttc ctacgaagaa
     2641 gagggtgtga tctatatggg ttccgtgaat gccggatata tttatccggg agcaagactt
     2701 cgggtgtccc cgggacgcga actgtatcgc gggattatag agtggaatcc ggatattgta
     2761 cattcccagt gtgaattttc tacctttttc atggcaaaaa agatcgcaga ggaatgtaag
     2821 atccctctgg tgcatactta ccatactgta tacgaagatt atacccacta tttttcccct
     2881 tacaagaaat ggggcaggga tatggtacag ttcctgacgc gacagatctc cgagaaggtg
     2941 gacagtatga tcgctcccag cacaaaaata gagacattac taaaagacta tggcattcat
     3001 tgtccggtat cagtgatccc atcggggatt gatctgagta aatatgacgc gcagacccgg
     3061 acggattccc gggagagaat ccgaagaaaa tataaaatgg accggaagac cacagtgctt
     3121 ttatatgtag gtcgtctggc aaaagagaag aatgtggaag aattgctgga gtaccagcag
     3181 aaagttcagg agagcggcac aatactgatg attgtaggcg gcggtcctta tttggagact
     3241 ttacggaaaa aggcagcaga gctgggtgtg acgggaagcg ttatttttac cggaatggta
     3301 tcacccgctg aagtagcatc ttattatccg gcaggagatc tgttcgtcag cgcttccacc
     3361 agtgagaccc aggggcttac ctatgcggaa gcactggcag caggattacc gttattatgc
     3421 agaagggatc aatgcctgcg ggcggttgtg gaagagggaa aaaacggctg gcagtaccgg
     3481 accgaagagg aattcttgaa agacctgaag aactggaagg agaaagaaga cgatgaacgc
     3541 agaggaatgt gcagttatgc aaaacagtcc gcagaaatat tttcccaaaa aagatttgcg
     3601 ggaagtatgg agcaactata ccagcggcag atcgaagaaa aacaggtcga aagagaaaaa
     3661 cacctggcaa agggccatca atattgcatc ctgggttagt ctgatcctct gtggaatact
     3721 ggcagtctgg ggctatcaga gcggcatttt ccaatcggtg gagaccatgc agcagtttgt
     3781 gaaccgcttc ggaatgatcg gtgcactgat cttcgtgctg atccagatcg ttcaggtggt
     3841 gtttcccatc ataccggg
//
LOCUS       DAWEJW010000167         3794 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_6552_length_3794_cov_44.772934, whole genome shotgun sequence.
ACCESSION   DAWEJW010000167 DAWEJW010000000
VERSION     DAWEJW010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6552_length_3794_cov_44.772934"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1115 a          749 c          982 g          948 t
ORIGIN      
        1 tgagaactgg gatccggtac aggaataaaa atagaataag aaaatgcgac aaggaacccg
       61 ggcgaaagct tccgggtcct ttttagaggg aaagtgtttg tagttagctt attgggtgag
      121 aggaattctg tggaccaccc tgcgatagag cttccacagg agcacagcag ggaaactgat
      181 tgccagatat gctgtggata aaagtccggt agcaccgcct acgattgcaa taattaaaat
      241 tccgatgttc atgatggatt cctcgctttt ttgtcagtct gtaaagtgct tgctgattcc
      301 ttcaaaaact ttacatatcg tattatagta aacggatctc cctgggtgaa tagttttgcc
      361 agacccttaa cactattttg tcgggttatt tacagaatct ttgcaaggca tttacagaat
      421 cttaacaggg cgggaacgat cccgggagta aggtttgcaa tcgcaggaaa tatcaagtat
      481 aatagagaaa attgactgtt acccggggtg gaatagtcgg agaaaataaa acggaggtag
      541 gaatatgagt atgacactgg aacaggctaa ggagaagctt gccaagtatg gtcaggaaca
      601 tgttctgaag tattacgggg agctcaacga ggaagagaag caggggattc tggatcagat
      661 cgaaactacg gatatgagca ttctggaagc ttgcaagcat aaagaagatc tggcaaaaaa
      721 aggagtgatc acaccgttgg ctgccatgca gttagacgaa atcgaagcaa acagggagga
      781 atttaccgca accggtatcg aagcgatccg acagggcaag gtagcagcgg tccttctggc
      841 cggtggtatg ggcaccagac tgggatctga caatcccaag ggaatgtaca atgtgggact
      901 gactcatgaa ctgtatattt ttgaatgcct gatcaataat ctgatggaag tcgtgcatca
      961 ggcagacagc tggatccacc tgtttgtgat gaccagcgat aagaacaatg acgcaacgat
     1021 cacattttta aaagagcatg atttcttcgg atacaacgcg gattatgtcc acttcttcaa
     1081 acaggagatg gctgcggcta ccgattatga aggtaagatt tatctggagg agaagggcaa
     1141 gctgtccacc tctcccaacg gtaacggcgg ctggttcatt tccatgaaaa ataacggcat
     1201 gttggatatc gtacataacg aaggcgttga gtggctgaat gtgtttgcgg tagacaatgt
     1261 actgcagcgg atcgctgatc cctgctttat tggtgctacg atccagagag gctgtgtcgt
     1321 aggctccaag gtggtccgta agaatgcacc ggatgaaaaa gtaggagtta tgtgcttgga
     1381 ggacggcaga ccttccatcg tggagtacta cgagctgact gatgaactga tgaatgcgaa
     1441 ggatgaaaag ggtgagcctg cttacaacta cggcgtaatc ctgaactatc ttttcaaggt
     1501 acaggatctg gagaagatca tggaagagaa gatgcctctg catatcgtgg agaagaagat
     1561 cccctatctg gatgagaacg gagaaccggt gaagccggaa agccccaacg gttataaatt
     1621 cgagagtctg gtactggata tgatccatca gatggacagc tgtctgccct tcgaagtcgt
     1681 cagaagaaaa gaattcgctc ctatcaagaa caagatcggt gtggattctg tggagagcgc
     1741 cagagagctg cttaccgaaa acggagtggt actgtaaaca tttcgacagg ttcttataac
     1801 ataaggttat tgagaatctt atggaattac agtaagctga taccgtaaga aataaagaag
     1861 ttgtttcatc catatccatt ggatgagatt agttgcatag ctgctgcagt aaacaaaagg
     1921 tattgtatac tgcacggcgg ataggagaga gtgtatgaga attttggtaa caggtggtgc
     1981 cggattcatc ggcagtcata ccgtagtgga attacagcag gcaggctatg atgtagtcgt
     2041 attggataat ctctgcaatg caagtgagaa agtgatcccc agagtagaag ccatcacagg
     2101 caagaaggtt cctttctata aggcagacat tctggaccgt gaagctctgg aagagatctt
     2161 cagcaaggaa cagatcgacg cagtcattca ttttgcagga ctgaaggcag tcggtgagtc
     2221 cgtacagaag ccctgggaat attatgagaa caatattgcc ggtaccctga ctctggtaga
     2281 tgtcatgaga aaacatggtg tgaagagcat tatcttctct tccagcgcta ccgtatacgg
     2341 caaccccgca tttgttccca ttacggagga atgccccaag ggacagtgca ccaaccctta
     2401 cggctggacg aaatctatgc tggagcagat cctgacggat atccagaagg cagatcccga
     2461 atggaatgtg attctgcttc gttacttcaa cccgatcgga gctcacaaga gcggtaccat
     2521 tggcgagaat cccaacggta tccccaacaa cctgatgcct tatatcacac aggtagcagt
     2581 cggcaagctg aaagagctgg gtgtcttcgg tgatgattac gatacccccg acggaaccgg
     2641 tgtacgtgac tacatccatg tcgttgatct ggcaaagggc cacgtaaagg ctctgaagaa
     2701 gattgaggag aatgcaggac tgaagatcta caatctggga actggtgtgg gctacagtgt
     2761 tctggacatc gtgaagaact tcgaagcagc taccggcgtg aagattcctt acgtgatcaa
     2821 accccgccgt gcaggcgata tcgccacctg ctattccgat gcttctctgg cagagaagga
     2881 acttggctgg aaggctgaga acggcatcaa agagatgtgc gaagactcct ggagatggca
     2941 gaagaacaat ccgaatggat atgaagaata atctatagtt tagtaaatgg aaagttagca
     3001 tagctacgag ccatgcagaa atagagtgga acagcgtcga tgggagcttg cgcacaagcg
     3061 acgctgtttc gctctatttt tgcatggttc gtagcgtagg gctgtcatgc aagggagcct
     3121 gccgcaaaca tcctgttcat tccatcctgt ctttcaatac ttttcgagaa aaaatcacca
     3181 ccttccgaca atcattcttg gaaggacatt ccatctgacg tgggcgtttt gtgctttctc
     3241 ataatatctg tacactatag gcatttctat tgctttggtg caaatcgcat caaaatagca
     3301 aaatcagtac aaaaaaactt ctaaatcaaa cattttttgt actgatttta ttatttttcc
     3361 cattttagcg aaaataagct cttataaaag gccaaaatct cctgcttttg tattttttta
     3421 gcccaaaatg gtattttcaa tacaaacaga acttccggag tggtggcagc ccatactaca
     3481 gctggttgtt gacattttct taaaaagtgt tagaaggcag gggcaatgcg gacacgatgt
     3541 cagccggaga ttttaagctg tggtatggga tgcttgagta tacagtgcag gagcaaattt
     3601 gagtgtgaaa aatcttcgct ttacatttag aaacttttta cgttgataag cttatcatta
     3661 agagaaaaac tgtaaagtgc attatttgca gacggaggaa aatgtctgta tggtccggaa
     3721 tacctgctct gcggtatctg ccatattggt catggcattg tcggtatgca tagcctcatc
     3781 cagggatacc acgt
//
LOCUS       DAWEJW010000168         3768 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_6604_length_3768_cov_35.726367, whole genome shotgun sequence.
ACCESSION   DAWEJW010000168 DAWEJW010000000
VERSION     DAWEJW010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6604_length_3768_cov_35.726367"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1072 a          713 c         1020 g          963 t
ORIGIN      
        1 gggattatca tcccactttt atgctcatat cggggcgggt cccaaggtct ttcgcccaca
       61 tatgagcaag ctcatgtggg cttagacctt ttgaccctgg catcatagaa tgatgtcatg
      121 gctgtctgtg cgcagtgcag gcagtctgta agggggagat tacttatgat ttggcaggac
      181 gaaggagaaa ataggatatt atgggtaaga aaaagcagat gcgttctgaa gtaaaaggaa
      241 atgtgaaaaa cagcatagga agaatcgctt ttgcagcatt ggcggtactc ttacagatcg
      301 gctggatact ggtattgatg atcaaactgt atcagtattc ttcagtgatc tccctgctga
      361 ccagtctgtt tgcgctggtg gtggcgctca ggatttatgg gaaacatacc aatgccgcat
      421 ttaagatgcc ctggattatt gtaatattgg catttcctgt attcggtctg tgtatttacg
      481 gattgttcgg gcataaagag gctatgcata aaatcatccg gaaatttgag gatgtggatg
      541 cacaactgat tcctctgaat gtgcaggatg agaccatatt gcagcagctg gagcaggaag
      601 atcctctgct ggcaaatcaa tgccattata tctggaagta tggtaattat ccggtgtatg
      661 acacgacagc tgtgaaattc tatccggaag cttatctggg atacgaggaa cagcttaagg
      721 agctggagaa ggcaaagcac tttatattcc tggaatatca tgccgtagag gaggcagagg
      781 cttttgcacg gctgaaggat gtattggcaa gaaaggctgc cgagggcgtg gaagtccgta
      841 ttctgtacga cgatgtgggc tgtatcggct ttttggataa aagctttata gaccgcatga
      901 atgccatcgg tgtacagtgc cgggtattta actatgtagt gcccttttta aatattttca
      961 tgaataaccg cgaccatcgt aagatcatgg tcatcgacgg aaaagtagga ttcaccggtg
     1021 gatataatct ggcagatgag tattttaata tcacgcatcc ttacggctac tggaaggata
     1081 ccggtgtgaa gctgaccggc agagcggtac agaattttac catgatgttt ttggaaatgt
     1141 ggaatgttat gggacaggcg gatacagatt atgaaaagta tctgaaggca ttgcaggaag
     1201 gtgcggaaga tggaaatgtg cagaaggcat ccggctatgt gcagccctat gcggattctc
     1261 cactggacgg agagccggtg ggtgagaatg tctacctgaa cctgatcaag acggcgaaga
     1321 aacgcctcta tgtggcaact ccttatctga tcatcagtga tgagatgacg agagagctgg
     1381 gactggcggc aaaacgtggc gtggatgtac gggtatttac cccaggtatt ccggataaaa
     1441 aaatcattta cggtgtgacc agatcctatt attccggact ggtgcgtcag ggtgtgcggg
     1501 tttatgaata taccccgggc tttttgcatg cgaagcagat gctgtgtgat ggggatacgg
     1561 cgaccgtcgg tactatcaat atggattacc ggagccttta tcatcatttt gaaaacggtg
     1621 tgtggatgca cggctgtgat gccatccggg atattgaggc ggattttgat aagctgttac
     1681 aggatagtga ggaagtcacc gataaatacc gggatggaag gaaaaatatg gcagtgagag
     1741 gctggcagtg tatcatgcgc ctgatcgcgc cattgctgta acagacataa taacggaaag
     1801 ctggagatta cgatgaaaat actggtcagt gcctgtctgc tgggcgaaaa ctgtaaatat
     1861 aacgggaaaa ataattacag cgagcgtgtg gcaaaatatc tggaagggca tgaggtgatc
     1921 ccagtatgtc ctgaggtgct gggaggactt ccgaccccga gggatccgtc ggagatcgtg
     1981 agcggtgagg tcataaactg taaaggcgta aatgtggaca ggcagtttcg ggaaggcgca
     2041 gaggaggctc tgcggattgc aaaagagaat ggcatcgatc tggcaatctt acagtcacga
     2101 agtcccagct gcggcgtaag gcagatttat gatggaagct ttaccggtaa actcatcccc
     2161 ggacagggag tatttgcaca attgctggaa aagaacggct tccgggtgat gaactgtgag
     2221 gaaatataat ggcaaaaata ttattgtttg gcgtgagtac acaaaaagac cgggctgtgc
     2281 gtgtggcagc ggagaaaata ggtgtggatg tgacggcggt gaaccgcagg gactatggac
     2341 agaagatcgg cacactggcg gaaatcagcg gttttcataa gaatggaaaa atatataacg
     2401 gtcctgattt tgcaatggaa atgctggtgt tttccggaat ggattcgcaa caggtggatg
     2461 cattcctggc agaatataag gcaaccggct taccaccggt tgccctgaag gcgattgtga
     2521 caccgtataa tgtctcatgg acagcggatg cgttgcttcg cgagctgatg aaggaacaca
     2581 ttcagcttgg aggataggga ataaagaaaa ttacagcttg tggtattccg ggctgcgttt
     2641 ttcatagatc gtataatatt tgaaaccaca cagctgcaga atttcggagg cgagatccag
     2701 ataggatcct acatcctgtg ctttgtgggc atcactgcca acggtaatga tctcgccgcc
     2761 cagttcgtga tagcggcgga gaacatcggt acagggatga ggctgtctga gaccatagcg
     2821 gaagcttccg gtattcaatt ccaggccctt gccgctgtga accaacagct ccagaatcgg
     2881 atcgatggca tccttatatt tgtcatagga ataattttta tctttgttcg gtgccacacg
     2941 gacgatataa tccaagtgcc ccaagacgtc ataatcgtca tggcatttca gattttccag
     3001 cgtctctgca aaataagcac ggatggcttc ctcctcggag gaaaacttct caaaatattc
     3061 ggggtaatag ggatccattc cccggcacag gtgggtagag ccaatgacga aatcaaaatc
     3121 ataggcggaa gtaaaatctt cgtcttggtc ggccagatgc ggctgcaagc ctaattccac
     3181 accgaagaga acccgcattt tcccttcgta tttggcgcgg cagcggagga tgtcatacaa
     3241 ataggaatct gtattcagca aaaagatact gccgggacct tcctcagtgc gtggataatc
     3301 aaaatcgtta tgctcggtga aacacatggt ttccagtcca agagagatcc ctttatcgat
     3361 catctccggc atgggagcct ttgaatctcc ggaaaaagaa gagtgaagat gacaatctgc
     3421 tttgatagac atatttttta tcctttctgg ttatcattct gttgtaagtc atttacaggg
     3481 tacagtatag caaaaaacgg agcaaaaatc ataccatttc taaacaaact gtaaaattta
     3541 ccacaaaatc acaaatattt tgcatttaca tcttgaatta taaagaggaa ttaggtaaaa
     3601 tgaatttgct gataaaattt tgacaatctg tgaatcacag taagtcaaaa gatataaaat
     3661 taatatttta ggaggaaact aaaatggcag taagagtagc aattaatggt ttcggccgta
     3721 ttggtcgtct tgcattcaga cagatgttcg atgcagaagg atatgaag
//
LOCUS       DAWEJW010000169         3729 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_6675_length_3729_cov_66.466794, whole genome shotgun sequence.
ACCESSION   DAWEJW010000169 DAWEJW010000000
VERSION     DAWEJW010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6675_length_3729_cov_66.466794"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1013 a          860 c          968 g          888 t
ORIGIN      
        1 gcttcggtag cagcggcttc ggtagtagcg gcttcggtag tagcagcctc ggtggtggca
       61 gcagcggtag attcagcagc aacgtctgca ttgctgtttc cacatgcagc gagagacaga
      121 accagagcca tagccatggt taatgcaagt gtcttctttt tcataataat cctccattcc
      181 tgcgcttgtg gcgcatatgg gtctgtgcag atacacagac atctcctcaa tttatccggc
      241 gcattcagac accgaacagt tccattatac ctgaaatgca agataaatca agattttttg
      301 caaaaagagc cccatgcgca ggataatacc accagatatt atcttacgaa tggggctgaa
      361 aaattacttc tttctgtttt ttaaatattc ccgccctaca tacaggagga tcagaagcca
      421 ggtgactgca agtgtgctta acaggatcac ggaagcccgg ggcatggcac ccagttcctt
      481 tttgccgaag gaggcatcac ttccctgacc gagacggtac aggctttcta catcgctgta
      541 aagcttttgc atgtaggtat catccacagt ctcttttctg cgggtggact tcaccacgtt
      601 ttcaatcaac tgtccggagg catcacgtaa ggatgcggaa ccgttaaata ccggtgtaac
      661 gaaggtgttg tctacgttgt caagcaacag tttggaagcc tcaattttca ccgcataatg
      721 cgtactgtta tccgcaccgc attcggacag ataatcctga tactctgcgg agttctgtgc
      781 cttagaggtt acaggtacat agccttctgt ctcggaataa gcaatctgca catcgttggt
      841 gagcagatac tgggcaaaca gccaggatgc cagtacttcc tgggagtctg atttattgaa
      901 gacgcagacg gaaggaccct gggagatcat ttcgggatgt tccgggtcaa actgtggaat
      961 catccgcacg gcggtctcaa actgcaccag agaatcctcg ctgatatccg acagcggagc
     1021 gtccgtaccc atccaggtgg ctccggcggt ggaatcaatg gcgaaaatac attgcccggc
     1081 attcaggaag ttcgcaggat aaccggagat cttgaaggtg gaaaaggcac cgctttttac
     1141 atgtccggca ataccataca ggagatccgt ggtagtatcg ttgaaaagtt caatgctgcc
     1201 gtcagccgtg gaatatcccg cattcttttg ctttaacatc tggatcatca tgttgtcggt
     1261 ggacttgtag ataaatggga tcagtacatt ctgcccgttg accacatagg tgccgtccgc
     1321 attttgggcg gtggcagcct cggagacttc ccagatgaag tcccaggtca gtacatccgg
     1381 cagagtatat ccaagatttt ccacgtaggt cttattcacg tagcaggcct cggtggaacg
     1441 catgtaagga acggcatagt aatgaccgtc gatggagcat tcattcaaaa actgaggaat
     1501 gacttcttct ctggcaggac cgtcataggc aagatctgtg ccgcccagac cgtatttttc
     1561 atcggcaaac aggtcatcca gaggaactac agtattctgc ccggtgaggt aggtggcaat
     1621 gtgatccggg taggtgatac agacattggg tgtcgtattg gtggaaatat tggtaatgac
     1681 atcgttgtaa attttgccgt aatccgtgta tagattcagg ttcaccgtga tattgggata
     1741 cagggcttca aaatccgcaa tggctttctt gtagatttcc gtctgggttt tgttggtatc
     1801 atttttcgcc cagaaggtga tctcatagtt cctggaagta tcgaactcct caggaacagt
     1861 gaattccggc aggcctttcg aaccatggca gccggtcagg gacagagaca gagtgcccac
     1921 ggcacataat aatgccagtt ttttgctgaa tcgactcatc ctttggtacc tcctctggca
     1981 acacctgcca tgactttttt acggaatacg aggaacaaca ccaccagagg gacggaaatc
     2041 acgaccaccg cagccatcat ggcggggatg ttctctctgc cgaaaccatt ctcacggatc
     2101 tcctggatgc cgttggatac cagatagtaa tccggatcat cggtgatcag tctgggccac
     2161 acataggagt tccagcattc aataaccttt aggatcacga tggtgatcag cgtgggcttg
     2221 cagatgggca ccatgacctt gcgcagatat cgcagatcgg aagtaccgtc taccttggcg
     2281 gcataataca gctcgtccgg aatctgtgcg aaattctccc gcagcagata gatataaaat
     2341 acgctagtca cggaaggcag gatcagaccg gtgaaggtat tgcgcagatc cagattcgtg
     2401 atggtggtga aattcgtaat gaccaccagc tcgttcggaa tcatcatcag ggataaaaat
     2461 aacgtaaaga tcagatcttt tccggcaaag ttcaaccttg caaacgcgaa ggccgccaga
     2521 gtgatgacca ccagcataat ggcagtggtg accacagtga aaaacaaagt gttccacagg
     2581 tatcttccca gagaaacagt ggtaaaagcg tccacgtagt tctgcaaagt gggagacagg
     2641 gtgaaaaact tcggaatata ttctgcatta taggagccgt agctttttac ggaggtcagt
     2701 atcatccagt aaaaagggaa cagtacgatc agtgcccaca gacacagaaa aaagtagacg
     2761 atcccattgc ggatattgcc gcggatccgg gtgcgtttct cgattttttc ataatcagat
     2821 gcaacatcag acatcacaaa gacctccttt cctatacata gtggacatgc tttttgctga
     2881 ccagcagatt gatgcaggtg atggtcagga cgatggcaaa cagcaggatc gccgctgcgg
     2941 aagcatagga aggatagccg ccgctgtcac cgtagagcat atcgtagacg tagccgacaa
     3001 tggtattcat ctctgcggca tttaagttcg tgccgaacag cgccacggca tcactgtagg
     3061 ctttgaatgc gccgataaag ccggtgatga tcaggtagaa taccatgggg gagatcatgg
     3121 gcagtgtaat cttggtgaac atccggaaac gggaagtgcc gtccactctt gccgcattgt
     3181 agtacatggg gttcacactg gcgagagcac tggtcaggat cagaatctta aaaggcataa
     3241 cgatccagat ggtgtaaaag cacagcacga acattttcgc ccagtagggt ccgtcgatga
     3301 aatccacgga gctgccgccg aaaaactgaa tcagcagatt gatcagaccg tcggagtaag
     3361 gggtcttttt gaaaagaatc atgaagacca gaccgaccgc cagagtattg gtcacatagg
     3421 gcaggaaaaa tatggtctgg aacagatccc gcagcggttt gatggagctt aaggcagcgg
     3481 agatcaaaag cgccagtccg gtggagaccg gaacggtgat gatgaccaga atgaaggtat
     3541 ttttcaatgc ctgtaaaaag tacggatcgt ggagcacata ggagtaattg tacattccta
     3601 cgccgtaaaa ggtttgggat gcggaattgt aaccctcttc aaaggagtac acgaatacgt
     3661 cgataagagg atagatcata aaagcaccca gaaacaggaa tgccggcaat agatagagcc
     3721 aggctttca
//
LOCUS       DAWEJW010000170         3637 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_6845_length_3637_cov_55.405918, whole genome shotgun sequence.
ACCESSION   DAWEJW010000170 DAWEJW010000000
VERSION     DAWEJW010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6845_length_3637_cov_55.405918"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1700..1799
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          898 a          927 c          745 g          967 t
ORIGIN      
        1 ccgcggatct tcgccgtgat ggtgccagac tgtacatatt gtgccgccac ctccggaagg
       61 gaatagttca taaatgtgtt ggagaaaaat gtcagcacta acattacaga caagaatata
      121 atggctgcat ttttaaccca ttctcttctc ttttttccac cgttctcgtt catcttgctt
      181 ctcctctata cttctctttc tatctgtact ccgacagtcg gatccatgcc ggtatatcct
      241 caaaatcagt gatcctagcc tttgacaccg ccctggtagg taattccgtc taccagatat
      301 tcttctccgt agagaaatac cagaagactg ggcaccatgt agatcgtagc cacggcgaat
      361 gccagtccga tctctccgga attgatcttg cttaagaata cggacaaggg atgcatctcc
      421 gcatcggata acaggatcag cggctgttcc accatattcc agtaatcgat aaatacaagg
      481 atggctgcgg aacacagcgc acctttgcac aatggcatac agatcagccg atagatctgc
      541 cactctccgg caccgtcaat ctgtgccgcc tccatgaccc ccacaggaat tctcttcatg
      601 aacttcgtca gcagatagac tgcaaagggt gaaaagatcc cgggcagcca gatggcccag
      661 tacgtattta atatattcag cttttccgcc accagataat tgggtactag cgttacctga
      721 tacggcatca acatcaatat cacataggaa aaaaatacca atgacctcag cttccccgag
      781 tacctggcga atccataaga cgccagcgat gccacgatca gctgaaacag tacgatcggt
      841 cccaccagga tcacggaatt ccagaatttc agcagatatt ccgggctttt caaaagcact
      901 gtagtatact ggctgaagga tacgatatcc ggaataaatt tcaaattcac tttttcggaa
      961 atatagacct tgccgccctt atcattcgtg gcaaataccg aaccatagtt cgctgatatc
     1021 tccgaagatg acatgaaaga attggtgatc gtcagaacga tcggcatcag gaacagtatg
     1081 gcaaaaaagg ctgcgatcac tgttgctatg ctgatgcgga cattctcaaa tatcttcttt
     1141 ctctttttga accggattgc ccttctaagt tcaggcggta caggtttttt aagcttctgc
     1201 tccatcagcc ttccacatcc tttccgaaat gattttccac tgcgaacaga agtccgatga
     1261 tcacgaccat gacgagagac atcagtatcg ctgctgcaga caatgtctga taatccagac
     1321 tcttgaactt attgttcatg aaatgctgca gcatatagat ggaatcgtaa ggataatctg
     1381 ttgtcagcag atagatctca cggaagatct taaaagagct gatcagggac atgatcgtca
     1441 caaacaaaaa agtcgatgac agataccgga tcttgatgta aaaaaaggtc tgtagcggtg
     1501 ttgcgctctc cagcctcgcc acctccagca gatcattggg aatgctggcc agggctgcca
     1561 tgaacaggat catattatat ccaagattct tccacaaaaa caggattacg ataacaataa
     1621 tggaatagct ggacttcagc cagtcaatct tctctacgcc gaagactccc agaagatcat
     1681 tgactgcacc gttataatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1801 cgccgtcctt gttggaggat ttggcggtaa tcgcatacag ctcactggcc tgcatataag
     1861 taccgctgcc accgccggag gtaggatatc cgatataggt taccggctcg ccgaacatag
     1921 cttcctcctc ctgaatacta ttcagctgat agatacctgt catatttaac agtacttcac
     1981 cggactggat cttcgccggt gtagatctgt cgtcattctg ccagtcatac tcttcgggga
     2041 aggtattgac aaattccagt accttctgga aatcttccga atcaaagctg cactttcccg
     2101 tctcccagtc aatatagctg tccagatcat aggctaacag tgtgtacagc ataccgctct
     2161 tggtcatgcc ttcaaacagg gaagcacctt catgctggcc tgcataggct ataatatcgt
     2221 caatggtcca gcccttctta tcacccactt cggaggtctt gccgacgatc gtgcttaatg
     2281 tgaagctctt cggaattccc accagttttc cgtcaaaggt ataactgtct acaatattct
     2341 caaagaaatc gtccttgctt aatacactgc tcttctccag atagggtgtc atatcctcga
     2401 atacaccctt agaagccagt tccttgacat ccaggttgga cagatccagg atatccgggc
     2461 atccggctcc ggaggtaata tcgttgttca tggctgtaat gccatccgcc caggaagttt
     2521 cggtccagtt attgtcatca atatatgttt taatattgac atgatattca ttactctgct
     2581 tgttgaaggc tacggctgct gcctgaaggc tctgggaggt atacagagtt ccgatcgtga
     2641 tctgggactt ctgtgccacc tcactggcct tggttttggt cagcttcaca aggtcagttt
     2701 cccctgtgtt ccagtcattg accactgcca ggatcttacc gtctgcggtg gctgcggcat
     2761 aggtaacata actgccgtta atatcactgt ccagccagtt caatatttcg gtagtcttct
     2821 ggtctgcgag actataatca tacagggcag tatccgtatt gaccaggaga tcattttcta
     2881 ttccggcaca taatcctccg ttaccgttcg tgttcgggaa gttatcatat gtctgtccca
     2941 atgctttgcc atcaaagtcg atctgggaga gcttcacatt gccggattgg tcataataag
     3001 ccaggtatac cttgccgtct tttgcctttc ccatgccctg gatccactgg ctgtccgtct
     3061 gcacagctcc ctgaaattgt ccgtcactgc caaacagccg gatcaatgaa tcagagctga
     3121 tataaaatct gccctggtcg tccaggcaca tgctgcccac ataggaattg ttctcatcct
     3181 gctgcatgat atccgtgata tcctgtgcca tcagttccgt accggatgca tcgtacttgt
     3241 gcaataccgt tgtctgctgc gtataagcgt catcaccttc tgtggcattc cactcaaatt
     3301 ccgcagtata aatattacct tcctcgtctg cctgcatgga atagatccca ccgccgtcag
     3361 cattaatggt gatcggcagg ttctccctgg aaccgtctgc cagcgaataa gctccaagca
     3421 cgaccttgct ctcaccggaa gcttcatccc attggtaatt tgtataatac agcttatctc
     3481 cctgtactgt catgttactg tagctggaat tcgtattatc atccaactcc aggtattcgg
     3541 gaacatatac atattccttc tgttctgtct gtccccccgg attcgatcca tcctgcctgc
     3601 cgcaggctgc aagcgacatt gccataacgg atgccag
//
LOCUS       DAWEJW010000171         3632 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_6859_length_3632_cov_43.651384, whole genome shotgun sequence.
ACCESSION   DAWEJW010000171 DAWEJW010000000
VERSION     DAWEJW010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6859_length_3632_cov_43.651384"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          802 a         1032 c          749 g         1049 t
ORIGIN      
        1 gggtatcccc gcagattaag aaccctccct ccgggaaata atagctgaca ccgcccgcgg
       61 tatgtccggg agttgccaga accttgatcc ggatgccgtc cagattcagt tcctcaccat
      121 cctgcagata cacatccgcg gtaaccgtta ccggacgccc catatctttc gacaggttgc
      181 tcttcgtatc ccgcaggaaa tctttctccg cctccccggc ataaattttc accggttccc
      241 aaccatgctc cccggcataa gcatccgcag ccgctttcaa ttccgcgcag ccaccaatgt
      301 ggtcaaaatg tccgtgggtc agaaaaattg ccactgtatg cagtccgtga tgcccaagtg
      361 ctgcctctat tttctttccg tgatccgccg ggtcgaatac cagcacctta tcactgtctt
      421 ctctgtacac aaaataacag ttggtctggt agatccccag tgtcatattt ccgatactga
      481 tattgctcat aatacactca ctttctgttt tctatccgga tcagccgcgt ttccaggcat
      541 cctgcattta acccgtggtt ctctcaatat caataacgct ctcgatgccc cggatcttgt
      601 caataatacg gttcagttcc tccctgccgc tgatctcgaa ggtcgtctgc agagtagcca
      661 gtccctgtct gttggttctg gtgttcatgg acatgatatc gatatttttc tccgtcaggg
      721 ccttggaaat atccgctaac agtccgttac ggttgttggc atagatcttg atctccgcca
      781 gatatttctc cgcatgtgcc tcctccggct gccattccgc atcgatcagt ctcgccctct
      841 ccatctccgg cagattgatg atgttgatac agtcggtacg gtggatagag atacctcttc
      901 cgcgggtcac ataacccacg atctcatccc ccggcacagg ggaacagcac ttggaaaaac
      961 gcaccgacag atccgcgata ccctttacca cgataccact cttggacttc atgagaaccg
     1021 gcttacccgc accattctgt gcatttaccg cattgttctc cgcgatgctt gccaggacct
     1081 cctcattggt catctctttc ttgtggtcac ggtcgtacag ttcctgcatc ttgttaacga
     1141 tctggccctc tttcagagcc ccgtgtccca tggctgccag tacggaatcc cagtcccgga
     1201 atccatactt tcgcataatg gcatccatat attcctgctt ctgcaggttg gcaagattca
     1261 cacctctggc cttacagtag gtggaaagca gctccttgcc ctttaaaata ttgtcttcct
     1321 ttaattcatt cttgaaccac tgattgatct tgttcttggc ctgggtactt tttaccacgt
     1381 tcagccagtc acggctgggg cccttggagt tctgggaggt aatgatctcg atccggtctc
     1441 cgttgttgat cttgtaatcg atggtcacaa gtttcccgtt gactctggct cccaccatct
     1501 tattgcccac cgcagaatga atgctgtaag cgaagtcaat cggtgtagat cctgccggaa
     1561 gattcttgac atcaccggta ggggtaaaac agtacacgct gtcagagaac aggtccagat
     1621 cattcttaag cagattcatg aactccttgt tgtctgacat atcccgctgc cactccagga
     1681 tctgacgcag ccataccagc ttctcttctt cctggacttc cggcttctta ccgtcggaag
     1741 cttccttgta tttccagtgg gcggcaatac cgtattccgc cacgcggtgc atctcgaagg
     1801 tcctgatctg gatctcgaaa ggctgtccgt tactgccaat caatgtggta tgcagagact
     1861 ggtacatatt tgccttgggc atggcaatat aatctttgaa acgtcccgga ataggcttat
     1921 acatctcatg aatcacaccc agtgccgcat agcagtcctt cactgtctcc acaatgatac
     1981 gcacagcgaa gagatcatag atctgatcca acgtcttatg ctggttcttc atcttcttgt
     2041 agatactgaa aaaatgcttt acacggccat cgactttcgc atcgatcccc gcatttttaa
     2101 tatgttcgct gacttcatcc acaatgccct ggatatattt ttcacggacg ctcttgcgca
     2161 ccgcgatctt atccaccaga tcataataga tgtccggttc cagatatttt aaggacaagt
     2221 catccagttc gattttcagc ttgctgatac caagtcgctg ggcaatggga caatagatct
     2281 ccagcgtctc cttggcaatc ctctgctggc tctccggcgt cttgtatttt aaggtacgca
     2341 tattgtgcag acgatccgcc agcttgatca taatgacacg gatatccttc gccatggcga
     2401 ggaacatctt acgcaggttc tccgcctgca tctccagctt ggcatccgac tgctctccat
     2461 tgccggacag cgggatcttc tccagtttcg tcacgccgtc taccagaagc gccacgtcat
     2521 ctccgaattc tctcttgagg tcatcctccg tcatcacagt atcttccacc acatcgtgca
     2581 gaatacctgc cacgatggtc tccttatcca gcttcagatc cgccagaatg atagccacat
     2641 tcaaaggatg gatgatatag ggttccccgg acttccggaa ctggttcttg tgtgcctcgc
     2701 tggctacctt gtaggctttc tcgatcatgg aaatatcatc actggggtga tatttctgca
     2761 cacagcgaat aagctcctga tataattcat ccggattcac gaattcttct gatttactta
     2821 tcttgtcatc atcaaaaatg acttcgttct tctcttctgt catagacgaa acagctccat
     2881 ataaaatatt tttattttcc ttcgtagcag atcgcagact ccagacgata tccgctcaga
     2941 cgctcacggc cctttaagcc tgccagttcg atcaatacca cgataccagc tacttctccg
     3001 cccagagatt cgatcagctt gatcatggct tcggtagtac cgccggtagc gatcagatca
     3061 tccacgatca gaaccttctg tccgggcttg atgctgtcct tgtgcatctc gatggtggcg
     3121 gaaccatact ccagatcgta agtggtggaa acagtctctc tgggaagctt gcccttctta
     3181 cggatcagta caaagggctt atgattattg tatgcgatcg gggttccgaa gataaatccg
     3241 cgggactcag ggcctactac cacatcatag tccagatcct tgatcttctc ctgcatggta
     3301 tcgatcgcca gatgcagtcc gtccgcatcc tgcaatacgc ttgtcacatc acggaaaata
     3361 atacctttct ccgggaaatc gggaatgctc cttacatatt cttccaactt tttcatgttt
     3421 ctacacacct ttctcttttt taacgaatta tatctgtttt tctgctattt caatctgttt
     3481 cttgataaaa tatttgatga tctcgcttcg ggtaataatg ccgataaata catttctgtc
     3541 atcgatcacc ggtacaaagt tctgcatctg tgcctgtccc agcagttctt ccatgttggt
     3601 atcgatacat accggccgga tcttgccact ct
//
LOCUS       DAWEJW010000172         3579 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_6978_length_3579_cov_58.830874, whole genome shotgun sequence.
ACCESSION   DAWEJW010000172 DAWEJW010000000
VERSION     DAWEJW010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6978_length_3579_cov_58.830874"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1679..1778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          795 a          774 c          948 g          962 t
ORIGIN      
        1 ctgggcgtgg ctgtgccgca gagaagtgcg gatccttgcc atgagctcgc cggtgccgaa
       61 gggttttgtg atataatcgt ccgcacccag atccagagcg gagacctttt cctgctcctt
      121 ggtgcgggca gatatgatga tcacgggaac atcgctccag gagcgcagct tttgcaacac
      181 atccaatccg tccatgtccg gcagccctaa gtctagcagg atcacgttgg ggcagaaaga
      241 agtgctgaga ctaagtccct ctctgccgtt taaggcatgc atgatcttgt agccttcccg
      301 gtccatggta gcggtgatga aattactgat acttctatcg tcttcgatta agagaacttt
      361 gatcttattc atatctgaga tccatatcct ttccggtatt ttcaacagtt ctatcgtatg
      421 tggctgagag tacacttatt tttccgaagc gagagcttcc gccttgggaa gtgtgaaaca
      481 gaattccgcg ccatccgcat gattcctgcc actcagcgtt ccgtgatggg cggtgatgat
      541 ggtcttgcag atggacagac cgatacccat gcctttgcgg gaatccgagc tccgggaatt
      601 gttgtaggta cccgtctcga acagattctg taatttttct tccgacagtc cttttccgta
      661 gtctctcaca ataaaagaga catgctccgg atgatcctcc acgatgaaat caatgggcag
      721 gatacttccg gaatgaacga tggcattttc cagcagattg atggtgacct gctcgatcag
      781 caccgcatcc atgggtatca tcagaaattc ctcggggatc ctgacccgga tggcggcatc
      841 gggatagcgt tttttcagct tctccaaagc ttctcccacc acttcctcca ccggctcctc
      901 tgtggtattg atgcttaagc tgtctccctg gatacgggtg acggacagca gattttccac
      961 catattcaac agccagttgg aatcctcgta gatattggtc atgatcgtac gctgttcggt
     1021 ggagcttaga tcgttctgac tttccagaaa cagggagctg ttgccgatga tgccggtgag
     1081 cggcgtgcgc agatcgtggg agatggcgcg cagaagattc gcacgcatct tttccatttc
     1141 ggcttctgcc agccgctttt cccgctctgc gatcatctcg gactggatgg tcatatgaga
     1201 ggtcatggta cacaggatca gagtgatccc actcatcagc aggaaggtga tagggtagcc
     1261 ggttagtgtg aaattaagct tataatatgg ataggtgaac agataattga tcgctattac
     1321 ggaaaaaagg gtgcaggcaa tgccgtagcc gtagcccacc gtccatctgg cgatgatcac
     1381 cagtgccaga atgtagaaca atgctacatt ggcatggttg tccgggacaa tatgctgata
     1441 taaaaaagag gcaagtgtgg cgcacagcat gcaggcggca cagatcagga tactgatggc
     1501 atgtctggta aagggtggtt ttaagcggaa gcttttccgc ggtgattttt tatcggaaaa
     1561 tgatcggaac acggtgtgat cctccatgaa atattgttag ttgtttttta aatttattat
     1621 aactatagta ccattacttt gcaatgcaag gtgtaaagaa gtagttaagg atatatgann
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta ggttatgttt ctgacaggat
     1801 tggcactatt gccggccatc gcactggtgg tatggattta tagacaggat aaggtggaga
     1861 aggagccgag gggattattg tggaaaatat tcctgttcgg tgtattgtct gtgatccccg
     1921 ccatgatact ggaaatcatt ctggatgaag tatttctggt atttatggat gcagatacgc
     1981 tgtgctacgt gatcctggat aattttatcg gggtggcact gatcgaggaa ctttgcaaga
     2041 tgaaagcggc caaatgggcg gcatggaagc atccggcatt caattataaa ttcgatgcca
     2101 tcgtgtactg cgtgacttcg gcattgggat ttgcagccat tgaaaatgtt ctgtattgtc
     2161 tgggcggtga ctttgggacg gcagtgacca gagccctgtt gtccgtgcct tcccatgcca
     2221 tcgatggcgt gatcatggga tatttcttcg gtgtggcgaa ggaggcagaa cttgcaggcg
     2281 ataaaaaacg caggaaacgc tacctgaagc tcagcgtgtt catgccgatg atcgaacacg
     2341 gtatctatga ttccgcattg tctctggatg cggactggat cttcgttttc tttttcctgt
     2401 ttgtgatcgt ggtggatgtc tgggcctata aatttgtgaa aaagcagtct gggcaggacc
     2461 gggagctgtc tgcataaaga ttgcgttaag aacccgtgaa aattttataa attcacgaaa
     2521 aacggtagga tcgggtcttt gcttcatgtt aagataaaga aaacctaaag aaaggaaaga
     2581 ccaatattga agttattgaa tccaaagatc cggcaaaaat ttgcagaatc cttgaaggcg
     2641 gtcctgccgg tagtagggat cgtgatcgta ttaagcttta tcatagcccc cattacctcc
     2701 agtatcctgc tgtgctttct ggtgggggcg gtgatggtca tggccggtat gatgtttttc
     2761 accctgggtg cggagatgtc catgaccccc atgggagaaa aagtgggtgc cagaatgacc
     2821 cagagtaaaa atattctgct gatcgtagtg ttatcttttc tgttgggagt ggtgatcacc
     2881 atttccgagc ctgacttgca ggtgctggcg acacaggtgc cttcggtgcc gaacatgacg
     2941 ctgatcctgg cagtggcagt gggcgtggga atcttcctgg tcattgccct tctgcgtatg
     3001 ctgatcggtg tggcactgcc gccgttgtta acttttttct acatagcagt gtttgttctt
     3061 gcatttctcg taccggagaa ctttcgggca gtggcgtttg attccggagg tgtgaccacg
     3121 ggacccatga ccgttccttt tatcatggca cttggtgtcg gtattgcgtc catccgtaat
     3181 gaccatcatg cggcagacga cagcttcggt ctggtagcac tgtgctccat cggcccgatc
     3241 ctggcggtca tggtgctggg gctgatctat aagccgacca atgcggatta ccagccggtg
     3301 gcgatcccgg agatctctga ttccgtggaa ctggcgcagc tttttgccca tgggctgccg
     3361 gattatatga aagaaatcgc actcgcatta cttcccattg tattgttttt cggtctgttc
     3421 cagatatttg ccctgcggct gtcggggaag acactggcga agatcctgat cgggctggtg
     3481 tatacttata tcggtctggt attgtttctg accggcgcaa atgtgggctt tatgccagcc
     3541 ggtaattacc tcggtcaggt catggcagcc agatcctac
//
LOCUS       DAWEJW010000173         3525 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_7097_length_3525_cov_41.555908, whole genome shotgun sequence.
ACCESSION   DAWEJW010000173 DAWEJW010000000
VERSION     DAWEJW010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7097_length_3525_cov_41.555908"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          870 a          911 c          776 g          968 t
ORIGIN      
        1 ggcgctccgc actgattcct ttttcgtgca ttcccacggg aataccacgt ccgttatctt
       61 ccacggttgc ggagccgtcg gcttccagtg tcacctttat cgtatcacaa tatccggcca
      121 gatgctcgtc cacagagtta tcaacgatct cgtagatcag atgattcagt cccttggtgg
      181 agacgctgcc gatatacatt ccgggtcttt ttcttaccgc ttccagacct tccagaacgg
      241 taatacttga cgcatcataa ttattgtctt ttgccatgaa atggaactcc ttatcaacac
      301 attattgtct taagtatacc atagattttg tgtttggaaa agtaaaaaat acaagggatg
      361 ggggaaacag ataagataat gaaaactccc ggaatactac tttccgggag taacagaatc
      421 attgaaaatt acacattgat ttttttacag catgccagtg caagggaacc ggggccgatg
      481 tggcaggcaa cacttaagga cagtggatcg atatgaatat cgtagccggg gaattccgcc
      541 tctacctcgg tgcgaaactg ctctgctgcg gctctgtcgt gggtataagc aatctgcagc
      601 cagatgtgct tgtcctccgt catgccgccg aaacgctcgt tgatgtcctt cttaatagca
      661 ttgatcatca tggttttgcc ctgcgaggtg gttctggcct ttgcaaaggc atccagcttt
      721 tcaccctgaa tggtcagcac cggcttcagg cgaagcattg taccgatggc tgcggccgcc
      781 ggagtgatcc gtccgccttt tttcagataa tacaaggtgt ccagcataat atagatactg
      841 ctgttgaatt tatcattttc caggaattct ttgatctggg ccgcgtcttt accggctgct
      901 gcaagctgca gggcatccaa cgcagactga cgctgggtta cggagatccg ctggttgttc
      961 acgacctgaa ctctgccatc ataatctgcc gcaaaggcca tggcactctg acaggaaccg
     1021 gacagaccgc tgctcatagg aatgtgtacg atctcgtcat attcctgtaa aagcttgtcc
     1081 cacaggctgg tgacactgtc aggagacggc tggctggtgg caatattggc accggatttc
     1141 agcttttcat agaactgctc ctgtgtcagg tcaatatctt catagtatgt gacttcatcg
     1201 atcataaaag gcatgggcag aacatggatt ccgcgacggg ctccttctgc ctgtgtaatt
     1261 ccgctgttac tgtcggttac aattgcgatt tttgacattc tggtatctaa accttccctt
     1321 tgcataaatt aacataaatt atctgatcct aagtataaaa ctttgaacat aaaaataatt
     1381 ttactgtcag atggtccgaa agtcaacaac atttactgcg gtatgttctt tgaaatagtt
     1441 caccaatgct cggctgtcgt catctgtcag ctccggatcg gatacaagca actgttccac
     1501 ggcatctgaa gcctctttta aaatatttgc atctgtatat atatcaccca gtacaaatgc
     1561 aaattcaccg ctctgccgga tgccgaagat atctcccgga cctcgcagtt tcagatcctc
     1621 ggaagcaata tggaaaccgt cgttggaatg gttcaggatc tgtaatcgtt ccatggtctc
     1681 tttggcttcg gaagtgctga taaaaatgca atagctttgg aattccccac gtcccacacg
     1741 cccacgcagc tgatgcagct gtgccagacc gaaacgctcc gcattctcca ccatcatcac
     1801 tgtggcattg ggaacattga tgcctacttc aattaccgtg gtggagacta agacatcgat
     1861 ctccttagca gcgaactctt ccataatacg gttcttatct gccggacgca ttttcccatg
     1921 aagataagcc acctgtacgg aaggaggcag aactgcccgg agcttctctg tataatccac
     1981 tacattctcc aggtcttccg attccccttc ttccaccata ggacagatga cataagcctg
     2041 tctgccggca gccacctctt ttgcgataaa ttcataagcc ttaggtcgat aggaagtccc
     2101 tacaacacag tttttgatcg gcagacgatt cgcaggaagt tcatcaatga cagagacctt
     2161 cagatcccca tatagaataa tggccaatgt cctgggaatc ggcgtagcac tcataactaa
     2221 cacatgaggc tcactccctt tggcggctaa tgtctccctc tgccttacac caaagcggtg
     2281 ctgttcatcc gttactacca gcgcaaggga agaatattcc actttttcct ggatcagcgc
     2341 atgggttccg atgatcaggt tagcctctcc ggaagcaatt tttgcataag cctcccgttt
     2401 ctccttcgcc gtcatagagc ctgtcagaag caccggacgg aaagcaattc catatttttc
     2461 tatatagcct gaaatggtct caaaatgttg gactgccagt acttcagtag gggccatcat
     2521 tgctccctga taaccattag ccgcacacat gagaagtgcc agcactgcca ggattgtctt
     2581 accggagccg acatctccct gaatcagacg gttcatggca taaggacttc ccatatcctc
     2641 cctgagctca ccccagactt ttttttgggc ttttgtcagc gggaacggca gttgctccag
     2701 aaatcttacg gtatccgcgg tctcatacat gggaaaatga ttctccgtct tcgccgccag
     2761 atctttattt ttccgaagaa ccagtaaaaa agaaaagaat tcttcgaata cgatacggtt
     2821 ccttgctgcc agcagttcct cccggttttc cgggaagtgc agtgaattga cagcttccct
     2881 gtgggaaagc atgggtattt tttgcaatac ggactgcggc agatattctt tttccggggg
     2941 ataatattcc agtgcctggg ctacggactt ctgcaccatc tggttggtca gtcctttggt
     3001 cagggcatat ctgggcagaa gccgacctga ttttttctga tattcctccc aggtaaacat
     3061 tctgggctgc tccatgatct tatgagtgcc cctctgctgt accagaccac ggaacagata
     3121 gaatccgccg ggttttaaca ccttttttaa aaatggcata ttgaaaaaag ttagctgaat
     3181 ggaatctccg ttgtcatccg tcagcacagc gttcaggatc gacagattcc ggaccttctt
     3241 cacattggga atggccgtaa tctgcccata gatggcctgc acactgccgg cagacacctc
     3301 ccggatggat accggagcat cgtaagtctc gtaatccctg ggataatgtc cgatcagatc
     3361 ctccacggta aaaacattga tcctaccaaa cagagccgcc gtcttctctc ccacaccttt
     3421 tattctgttc acaggtattg catcactcat cccagtccct cttttctatt taaattgaaa
     3481 tttagcgaaa tatccccgta gcccttggca gggtataaga aacgc
//
LOCUS       DAWEJW010000174         3477 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_7215_length_3477_cov_41.632379, whole genome shotgun sequence.
ACCESSION   DAWEJW010000174 DAWEJW010000000
VERSION     DAWEJW010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7215_length_3477_cov_41.632379"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1115 a          623 c          924 g          815 t
ORIGIN      
        1 ggatcaaagc attgaagatt gtcattgccg aactggaaaa ggctctggga taggaaaaga
       61 aagtagagaa cgtaaacacc gtcttgtaaa aaggcggtgt ttctatatgt gatgaaagcg
      121 ttgacaatgt aggattcata aaatataatt atttcatcga aacggtgaaa aggggtgaat
      181 tgatgaaaca aaatttatca caaatacaat atgagcaact ggccagagaa caattttgca
      241 accggctgaa agaaattccg tttgtttcgg atatcgaggt gtttttggaa aagaagaatg
      301 caccgctggg ggatttcaag gcaaaggtgt atttggagga cagcgatgag cctcttttat
      361 ttgtgattga agtcaggtct aacggagaaa agagattcgt taatcatttt atggaaatcg
      421 tgatgcggca ggatgataaa acgcattata tttttatggc accatatatt tcggagcagt
      481 cagccagagc gatgaaagag gaaaaatgca gttatatgga tctgtccgga aattgttata
      541 tcttaacgaa aagatttctg ctctatgtca gtggacagga taacaagttc ataatccaca
      601 gggaaaagaa aaactactta tccaaatcgt caagtgctgc atcagcaatt attcgtacca
      661 tgctgaatga accggagcga aaatggcagg tgaaaacgct ggctcaggag agcaatacgg
      721 cattgggaac agtatctaat gtgaaaagtt ttttgcttgc cagagactgg atagataaga
      781 gtactacgga attccgtttg cagaacatta gggaactgtt ttatacatgg gcaaaggatt
      841 atcatcaaaa agaagcgaga aaattacagt tttattcttt ggatacggtt ccggagatag
      901 aacaatcagc cgctgaatgg agtaaaacac atgataatgg agctatatta ggtggctttt
      961 cggctgcggc acgatatgcg ccgacagtac gttatcaaaa agccgaaata tatgtagaac
     1021 ctcaatttgt tcaggaattt gtaaaagatc tggaattgca gccggtcaac accggaggaa
     1081 atgttgtgat aacgataccg catgacgaaa ccccctgtat gtacgcaaaa cctgttcatg
     1141 atacattagt aacttctccg gcacaaacgg taattgatct gctgggggat gccggaagag
     1201 gggaagaggc tgcggaggca atcctgcgca gagaataccc ggagaggaca gaggatgaaa
     1261 gaagaactga aaaaggcaac tgactattca gagggacaaa agacagccgc atacatggta
     1321 ctcggagaga tcgtaaatct tttgaatgag ttttcagata atatcagaat cataggagga
     1381 tgggttccga cacttttata cccgaccggt gaccatatag gttctattga tgtagatgtt
     1441 ctgctgaatc aactggagat caaaaaggca gaaagctatc ggacgattaa acggatactg
     1501 acacaaaatg ggtatagaag acatgaagaa aaatatttta catttataaa aacaatagtt
     1561 ttagatggag tttcctatga cgtagacgtg gatttcctgt ccggaaaata tggcggcgat
     1621 ggcggaaatg taagcaaaca tgtggatggt attaaaacct taccggctac gggaggaaat
     1681 tttgcatttg attttccgcc aacggatgtg aaaatagaat atatgcggga ggatggagca
     1741 aaggacagtg gacatgtgaa agtgatttct gtggtgcctt atctggtcat gaagacagca
     1801 gcactgggga ggggaaaacc gaaggatgcg tatgatatat attacatggt tgacaaatat
     1861 gagggaggcg taagaacact tgttaaggaa tttatgcctt atacagacag agagttgatc
     1921 agagaaatgt gtcagaagct ggatgaaaaa tttgcttcgc cacagcatgc cggaccaaca
     1981 gatgtagtga cattcatggg aataacagag gaagaagaaa aagaacagat ccgacaggat
     2041 gtttaccaga agatacacta tttaacaaat aaattgaaaa caatgtaagc aaaatcttgt
     2101 gttggcagac aggtctgtta taatgataca tagttgtatt tcccagatag tatttacaga
     2161 acgagaggag aaatcatggg cgagaaccgt aagatatatg cagcattatt gggagcagga
     2221 accgtgggca gcggtgtgta taagctggca ggaatgcagg aagaagatat ctgcctgaaa
     2281 acaggagcac agctagagat caaaaagatc ctggtgcgga atccgggaaa ggtaagagag
     2341 ggaatcccac aggaactatt cacagatagc tggcaggaga ttttagaaga tcctgagatt
     2401 cagatcgtca tcgaagtcat gggcggcatc gaaccggcca gaacctatgt ggaagaagca
     2461 atgaaggccg gcaaacaggt ggtcaccgcc aacaaggatc tgctggcgga atatggggat
     2521 ccgttgtttg agatcgccac ggagaataaa tgcgacttac aattcgaggc ggcagtagca
     2581 ggcgccattc ccatcatccg ccctttgcgg cagagcctgg cgggaagcca attgacggag
     2641 atcatgggaa tcgtcaacgg tacgacaaac tatattttga ccaaaatgtc cgaggaaggt
     2701 atggggtatc aagaagctct ggatatggct acgagactgg gctatgcgga agcagatccc
     2761 acagcagacg tggaaggcta tgatgcggga cgcaagatcg ccattatggc atccatcgct
     2821 ttcagctccc gcgtgacgtt ccctcaggtt tatacggaag gcatcacgaa gattacagca
     2881 gaggatatcc ggtatgcgaa agaattcggc tatgtgatta aactgttggg tattacaaag
     2941 cttacaggta aaggaattga agtaaaagta catcccacat tgatcccgga cagccatcct
     3001 cttgccacgg tcagagattc cttcaatgcc gtatttgttc atggtcaggc ttgtgatgat
     3061 gccatgttca tgggacgggg cgccggacag atgcccactg ccagtgcggt attgggagat
     3121 gtcatcgatg tgatgcgtaa tattatccat ggaagttgcg gcaagattgg cagtgacagc
     3181 cacaagaaac ttctcgtact ggatgtggag gagacgaaga gccgtttctt cctgcgcctg
     3241 caggtagaga accgtgccgg agcattggcc aatatcgccg gagttcttgg taacaacgat
     3301 gtcagcatcg cccagatggt acagaaacgc agccacgacg gtattgccga gatcgtggtc
     3361 atcaccgatc ttgtgaccga gagacatttc aacgacgcca tccgcatcat ggaaggcatg
     3421 tctgttgtga gagaaatctc aggcataatc cgggtctact aaagtttgta gaattga
//
LOCUS       DAWEJW010000175         3386 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_7425_length_3386_cov_67.299310, whole genome shotgun sequence.
ACCESSION   DAWEJW010000175 DAWEJW010000000
VERSION     DAWEJW010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7425_length_3386_cov_67.299310"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1058 a          662 c          938 g          728 t
ORIGIN      
        1 accagactgg taggtattac cacggaggga atggcgagac agaagattct ggaaggcgca
       61 gatattacgg tgatctaccc cacggacggc accagtgcca taccggatgc cacagcaatc
      121 gtcaagggcg caaagcatat ggaaaatgct aaattatttc tggaatttac cgtcagtagt
      181 gatgtacaga gactggtgga ggaagccttt ttccggagaa ctgtccgcaa tgatatggaa
      241 gagtatgcag caccggagca gacaaaatta aaaacgatcg attatgatat tcattgggct
      301 tctcaggaaa aggaaaaaat tctgaacaca tgggagacgt tacaggggag aacagatgaa
      361 aaggtggatt aaaaagtcgt ttcacagaca gttgctggtc tgcttcggtg tagttgcgtt
      421 attgccgtta ttgctttttg gggtatccct gatccagact atggagacca agatcaacag
      481 cgattatgag aaaaaagtaa cagagcaggc ggaacagata gatgctggca tattggaatt
      541 gtttcaggag ttcgagacgg tagtggagaa tataaatgct aacacccgga tcgtggacca
      601 gataggtgaa gatgacacct ggtcgaagag caagatttac ctgcaattct atcgggaagt
      661 gactgattac agagaatatg cccaatttga tctgtatgat aaaaacggaa aatgtattta
      721 cactacggca cagggaagtg ccaagacaga tctgccggta tactggggca tcctgaaagc
      781 agtcgaggat agtaaagaga cgctggtgct gcggcgtgca gatactaatg acagcaagat
      841 tttattgtat gcagctggta aacttatagg aaaagacggt ataccggaag gttatattgt
      901 gatcagcatg cgcgcagaaa attttgaaaa agtgcttcat gataagggaa atgccaaagc
      961 ggaggtagca atcatggacc ccttctggag aaccatttat gataatggta acctgcagga
     1021 agagcagatc cgggaagaac tcatggttgg tcataagctg ctgggagtat atcgtgccgg
     1081 agaactgctg atccagccgc tgggagacag tggattgtat actgcattgt cctgtcccag
     1141 cgtgttttca acagaagtat tgaactccat gtacagtgtg ttgatcgtga tgctgaccat
     1201 cagtgtcatt ctgtgtgttc tggtggcttc ccggctgggt agaaatatga cccgtcctat
     1261 cgaacggatg aatacggcca tgcgtacact gcaggaagga gaccttaccg tcaggatcgt
     1321 ctccgacaga gaggacgagc tgggacagat gtcccggaac tttaatatca tggccaacga
     1381 actggagcaa agcgtgcagg ataaggtgga gaagcagaag gaactgaatg cctcccatat
     1441 tgccatgatg caggcacagt taaatcccca tttcctgtac aatacactgg ataccatgaa
     1501 atgggtggcg aaggccaatc acattccgga gatcgccacc atggcagcgg gcctggcgaa
     1561 gatcctgcgg accagtatct ccaagagaca gttcatctat ctgaaagaag aactggaact
     1621 ggtgaactgt tatgtagata tccagaagat ccgtttcaat gataagtttt tgtatgccgt
     1681 ggatctgcaa gagggactgg aagagtgcgt gatccccaag ctgatcatac agcccatcgt
     1741 ggagaactcg gtactacacg gattaaagga gagtgaagaa ggaaatatcc ttgtgcggat
     1801 cacagaacag gaagatgtcc tgtgtattga agtaaccgat gacggctgtg gcgctcccga
     1861 agaatggatg gatgccatca atcacagaag acaggagcag ctggtggggc atatcggggt
     1921 ctctaacgtg gataccatta tccggctgac ctatggggaa gaatacggca tccatatgga
     1981 gagtcttacc tcaaacgccg aaaaccctac ggacggacag cgaacagaag aggaaggcga
     2041 agtccacggc acgaaagtga cattacggct gccggtaaga tacggagaag cctgaggaac
     2101 cgatacagag caacgggcag agaggaattt ttaccggata gtggggaata tacagaaaac
     2161 gggaagaaat aatggaggga gtaatacgat gtacaaagtg atcgtgatcg atgatgagat
     2221 gctggtgcgc agaggaatcg tgatggagac agactggcag acactgaact gtgtcgtcgt
     2281 ggcggaagcc ggcaacggaa tcgaaggact ggaagccgtg cggaaatatc atccggatct
     2341 gctgatctgc gatatccgta tgcccaaaat ggacggcatc gaaatgctga aggaattgcg
     2401 ggcagaaggc aatgatgtca gtgtgatctt tttaactgct tacagtgaat tctcctatgc
     2461 gcagagcgcc ctgaaattat tggccagtga ctatctgtta aagccttttg gagacgggga
     2521 actggaacag gcagtgagca atgcactgga gaaacgtaag aggacacagg aaaaattgga
     2581 aaacagcaag gaggagccgc ttcccgaact ggtcttaaat aaaggcgata agagcaaata
     2641 tgtcatggca gccgtagact atatttccgc tcattatggg gatccggaac tgtgtgtggc
     2701 ccagatcgca gagcacctgg ggatcagtga aggacatttg agtcatacat tcaaaaggga
     2761 gacggattac acggtggcgg catacattac cagagtgcgt atgcgcactg ccatgaaact
     2821 gttgaatgac tgccggaata aggtgtacga agttgcagag caggtaggct atcgtgatgt
     2881 tgcacatttt tccagcagct ttaagcggat tgttggtgtt actccttccg aataccagga
     2941 tagaagcatg taaagctaaa aagatgcaca aaagaaacaa taaaatagca gaaaaactat
     3001 acatagtatc agaaatatta aaataataac agttttgcct tatacaaatt gctgaattcc
     3061 caacgtataa tagaatcatc agaacggaag tgggaaacat cccattccaa gtaagtggac
     3121 catgaagggt tatacaaact atatgtttca agggaggaca gcatatgaag aaaaaagtag
     3181 tatcgttact tatgatctgt gcactgagtg ttggtatgtt agccggatgc ggcagcagcg
     3241 agacccagaa ccaggcaact ggaagcgcag ataacacaca gacagccgga accgagacgg
     3301 tggcagagac caagaccccg gaggtagccg acgacgcaaa tcttactgca actcagcaga
     3361 tcatcaaaca ggcagaagga atgacc
//
LOCUS       DAWEJW010000176         3336 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_7546_length_3336_cov_82.439500, whole genome shotgun sequence.
ACCESSION   DAWEJW010000176 DAWEJW010000000
VERSION     DAWEJW010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7546_length_3336_cov_82.439500"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          756 a          679 c          715 g         1186 t
ORIGIN      
        1 aggacgtatc tggattctgg tgtccagcaa ttctgtatac tgttcaggtt caggacgaaa
       61 tttttcttcc cggaaagcgg cagcatcttt ttcaagctgt tttttgagcg agatgtcctg
      121 agaatctagg atatcaagat tgttcttcat ctggtcgtat ttcttggata gattcgtcat
      181 atctttatct gtagagcatt ctgcctggaa gattagctgc tctttccttg atttcagttc
      241 ttcgatttct tcggtaatag tggtaagctg ctgatttaat tttatatgct ggatgggatt
      301 caaaatactg gttttctctt ttttcacatt cagttctttc cgctctgtaa ctttagcttt
      361 gagtttcttt ttaacggtgt tgtatttatt cagtattgga ttaaaatgat tcatccagtc
      421 atggatcact tttttttgca tctcattgtg gagcaaatga tattgtgtaa aaatcatgtg
      481 atttcggatt gcttccaatg tttctgcaat tattggaata gacttttcta cagcttcagc
      541 cagttttgct acttttgctt ttaattctcg gagcattttg ttatccgcac gaatctgacg
      601 gttgatttca cagcggtctg ctatcatgcc tttcttttcc atattctggg caatgtagcc
      661 ttcatggatg gttggctgtt cggtgattcc ctgatctgca aagctgcggt gatcaatggt
      721 agcatttatc tgattacgtg caagcatttt atttacggca tctgcccagt ttgcccgcca
      781 gatgcaaagt tgttcatcac tgttccattg ttcggaaata ggattctgtc tgccatatcg
      841 gctgctcttg gggtgtttat caatccgttc atatcctttt tcctgtgcgg cagaggaagt
      901 cagatattct ttctttttcc caactttata gccatattgc ttttcccatc cctgtttctg
      961 agcagtttta aattcagaag cagtaaatcc tttttcttct ccatctttga tacagagata
     1021 ttctttttct gttttatact gccatgttcc attttcattt agtggtcgga cagtaagaag
     1081 aatgtgtgca tggggattgt gaccatctgt atcgtgaatg gcaaaatcag cacacatccc
     1141 catatctaca aaattctttt taataaaatc ttgaagaaga gaaatattgc cgtctttatc
     1201 caactcaaca gggagtgcga caacaaattc tcttgccagt cggctgtctt tggtcttttc
     1261 ggtttcttcc acagcgttcc agagttgttc ccggtcaatc catttggatg gagcaattgg
     1321 aggaagcatc acttcctgat agatcagccc atgctttctg gtatagtcat gctggatacc
     1381 atcataatca ttgtacatcc ggctgcagct catataagca gaagctgcga cagcggaacg
     1441 tccggaacca cggctgacaa ctttggcttg catatgataa attgccatat ctggcacctc
     1501 ctttcgatgt tgcctgcaac agcggatagc cctcggcaga gcgcacgagc cccgtagggg
     1561 ataccaccgc ccgatttatc gggtggtgag taagtgcgcc cttcgggagt agaacttgtg
     1621 cgtttacatt cctcgtagcc ggattatcag ctcccgtttc agggaatcca aatccatttc
     1681 tttaatatgg ggaacaatac tttccaaaat ggctccttcc tggactaatc ggtgtgttct
     1741 ggcatcacgt tcttttttgc gatttcttgc ttcggcttct tcttggcggt gtcttgcctg
     1801 taaaagtgct ttttcttctg gtgtcatgat ttttttatct gccatatctg gcacctcctt
     1861 tttatgcccg tatccgggca gtattggttt taaaatctta tggtttggta ctatagtttc
     1921 ggataactga cagtggcatg tttctggtga taaataagga aagaaagtag taccactgtc
     1981 agtgaagaat aattatcgtg ttatgcttct ttgcttcagt tctggttcgg agcatgattg
     2041 tttaacagaa ttctttttgc ctgttttaaa ttagctgttg cattgtcctg acgaatattc
     2101 cggttattga tacggacagg aatacatctc tccagaatac ggtcataaat tcgtttgtgt
     2161 gcgatatcag cggcgttatt cagctctgat aaagtgagat tggtcgttat gatcaggggc
     2221 tttttactgc ggtaacgact gtcgatcaca ttgaatacct gttccagtgc aaagtcagag
     2281 ttccgctcaa ttccgagatc atcaataatc agaagactgt agcggtttaa gctgttgatg
     2341 aactggtttc tgtcttcaaa atgcattccg gtaagggtat ttagaattcg ggaaaagttg
     2401 gtcatcagta caggaacgcc tttttccaga agggcattgg caatgcagcc tgcaaaaaaa
     2461 gattttccag taccgacatc accccagatg agaagtccgg aagcatttgc tctcatctct
     2521 tcccagttgc ttacataatt gtgtgccagt tttatttccg gattgatgcc gttatccctg
     2581 gcaaaagtgt agtcatagag tgctttgtcc tgtaagccgc tggttttaag atgctctatt
     2641 tccatttgtt tttcatgaag ttttctctgg gcttcttcct gctcaaagat ttcctgctgg
     2701 catttacagc ggatggaagg aataagaacc tttccctgta attcatgcct gcattgcctt
     2761 ggcgtattgc atttggaaca gtagatcagg tggtctgttt cgttaaaata ttcatcagac
     2821 tgaagctctc tgtttggggt tatagctgtt ggtttttcta taaattttgt cataatgttt
     2881 cgtattcctc actttcgtaa tttcgttgtc gggaagcagg atgatcctgt cttgcccaac
     2941 tgatgatggt agctgcgtgg ttctgatatt gctttccggt agaagccata taaccggata
     3001 agcgttcaat ataatccttc cagtcaggga ttgtctgccg gatatcttca agctcttttt
     3061 ctgacagaaa aacattttga aattttccat agggtgagag tggctcctta ctccctctta
     3121 ttgctaaatt gttctttttt atctctttct tattaccaga cagttttctg tctgtatcag
     3181 ggaaagaatc ctgtctgtca ataggacagt tttttgtctg tcttagggaa agaaatctgt
     3241 ctgtatagtg gatggtttct tttgggagtt ttacataaat ccgattgggc tgtccgggtc
     3301 cctgccgttt tcggaagata agctcctgtt tttcca
//
LOCUS       DAWEJW010000177         3324 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_7581_length_3324_cov_51.287856, whole genome shotgun sequence.
ACCESSION   DAWEJW010000177 DAWEJW010000000
VERSION     DAWEJW010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7581_length_3324_cov_51.287856"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          769 a          852 c          682 g         1021 t
ORIGIN      
        1 gcacttttat tccatataca attctaatcc agcatgccgt ctttgatcac attgtggtca
       61 cgcagcagtt tttccactac agtgcccttc actacgcgaa gcagcggctt cttcagtgca
      121 tttaaaggtc cgggcagttc aatctccaga ggagacatgc catccatcag cttcacgctg
      181 ctgtccaata actctctgac atcatataca cttccccata cctcaggcac acctctgtct
      241 acacctaaca gtccgtagac agcctccata gcggttctca cggaatactc tgtggtaaac
      301 acggtgtctc tcggggtctc tgcaaactgg cctaagaaag cgaagttcac acagccgtcg
      361 ggaatgacat cgggacggtc acccttggtt ctgggcatga agaatgcggt aatataaggc
      421 atcatggtag gtacgcagac tgcgctgtgc tctgccagat cctcgatatc ttctacgggc
      481 acacctaagt gatacagcca ctcttcggtg atctctttac cggtacaatc cttcatgggc
      541 ttcttaatat aatcgccggg tacgtcggta aacagaccgt atacccatac acatacctga
      601 tttttgtcct gttccttgaa ctgtccctga cggttgattg tccagctcat gagccacttg
      661 gaatcctggc agcttacgat accgccggtt acgaccttac cgcttctggg gtcacgcttg
      721 cagatattgg tgatataagg aatgatcttg tcatccagtg tggtcacggt agcggattcc
      781 cagttagtct tggagacatc agaacagaac ttctcgggat gtccgaagga aggatcctgt
      841 tttgcgatat ttttccacag gctccatacg ccgctggtgc gaacctccgc gtcaccgttg
      901 ggtgcatggt tctggtcacc gtagatagta ccctcggtac agcttccgtt ggtaacgaat
      961 accaggtcgt tctcggtgag gacaatgccc ttctctacac cttttacctt gcactcgata
     1021 gcctttgcca cctttttgcc gtcattgata tcaaaaataa catttgtcac ttcggtgttg
     1081 aactggaagt ccacacctgc tgcctccaga tacttcttca tgggaaggat cagagactcg
     1141 tactggttgt acttggtgaa cttcagggca ctgaagtcag gcagtccgga aatatgatgg
     1201 ataaatcttt ggaagtaaag cttcatttcc agtgcgctgt gccagttctc gaaagcgaac
     1261 atggttctcc aatataacca gaaggtggag ttaaatactt catcatcgaa aacatcttca
     1321 atggtcttgt catacagatc ctcgtccttt gtcatgaaca acttcatgat ctccatgcag
     1381 cccttctggc ttaagttgaa cttgccatcg gtatgggcat cctgtccaca atccttcgtt
     1441 gcacggcaca gagaatagtt gggatcttcc ttgttcagcc agtaatactc atccagtacg
     1501 ctggctccgg gtacttccag agagggaatg ctgcggaata gatcccacag acattcaaaa
     1561 tggttctcca tctcacgtcc gccacgcatg acgtagcctc tggtaggatc aaaaataccg
     1621 tcacaggcac cgcctgcgat atccattgcc tctaagatgt gaatatggga accgggcatc
     1681 tgtccgtcac gtaccaggaa gcacgctgct gctaaagatg ccagaccgct gcctacgatg
     1741 taagcgttct tctcgtccac tcctgcgggc ttcttaggtc ttgcgaaggc ttcgtagtta
     1801 ccgttggaat aatagatgcc cttactgttc ttgtcgtatc ttccaagctc ggtattgcgg
     1861 tagtctttct tggctgcctt gacagctgcc tttctctcct ctttcttcac ctctgcctta
     1921 tgtcccttgg ttactgctgc cactgcgcct gcacctactg ccagcgctgc cagtcccagt
     1981 ccgatattat tcttttttgc cataatgatt accacctttc tttgatgact ctcaaatgtt
     2041 tttctgttta caaatagtat cctacttctt ttttataaga aaaccaattt acaaaagagg
     2101 ataaatgata aagtttttat catcctgccc tttttgtaaa gtacttatac tatttcagaa
     2161 tttcttttcc gtgtgctcaa aattctcgat ggagcggctg atgtttccgt aaagagtgat
     2221 tgccagttct tcaacgatct tatgataatc gtccttcatt ccccggtcga tccagtctaa
     2281 catcacaccc acaaaaccgt atttgtaaaa tccggcaatg aattccttgt gttcctccgc
     2341 tacctgcatg ccggtgcatt tttcctccac cacgtccgca atgagctgat aggtcagctt
     2401 gtttaagtag ctttcgatct tctggcgatc cacgcagcgg tagatattca taatgaaagg
     2461 tttgttctcc tgcactgcct cgaaaatctg gcagaagcct tcttgccagt tctcgtaggt
     2521 cttcttcccc tgcagtgcct tcgtgccgtc ttccacgcag acccactcta ccagatcata
     2581 gatgtccttg aaatgatagt aaaaagccat ccggctgatg ccgcagtcct ctgcaagttc
     2641 gttaatggtg atcttatcca gagacttcgt gagaagttct tttttcaaag ctgcttccaa
     2701 cgccagtttc gttgtattgg ccatagcctg tcctccactg ttttatgtat tattttctgt
     2761 tcttcctcga tatagcttat gtaattctat tataagctca cgcattcgta ccatcgtact
     2821 ctacatctta cttcacagga tattcattcg ctaaggttgt cgttcgtcaa tcatgtaaac
     2881 atgtagactt ccttatactc attatattgt acgacaatag actcaaaatg ggcaaattaa
     2941 ctttttataa aaaaactctt gctttttgta aaattccatg gtattatctt acttgcgttt
     3001 cggggtgtgg ctcagcttgg ttagagcgcc gtcttaggga ggcggaggtc gcgagttcga
     3061 atcccgccac tccgattcat tgtaaagtcc ttgattttca aggacttttt tgttacattt
     3121 catgttgtat catggtactt ttctgatttc ctctatgttt ccttttggtg tttccctata
     3181 gccacatatg cgtattgaat ctttccgttt tgaagtcatc ggggtatact gtagaatttt
     3241 ggtagattac gccccactat cgaaacacta acggggctta tctgaaacat tttaaagatt
     3301 tacgccccgc tttcagaacg ccaa
//
LOCUS       DAWEJW010000178         3209 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_7861_length_3209_cov_69.681674, whole genome shotgun sequence.
ACCESSION   DAWEJW010000178 DAWEJW010000000
VERSION     DAWEJW010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7861_length_3209_cov_69.681674"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          948 a          689 c          919 g          653 t
ORIGIN      
        1 agaaacattt gtttcaaagc cacgagtaac tggttgtacg tgtggaatgc agaagggaca
       61 gaatcttttt tgcattcgtt tcttatgtgt acaaaccctt tacctgaaat aggaaaaggg
      121 ttttattttt ggtgctgaat agctataaaa catgtttctg acttacatat ttaagaacac
      181 cggggcaaac cccaaaaatc aaaagagaca gaaaaactgt cagaggagga aacatgaaaa
      241 aaagaatcgt agcagtagta ctggcaaccg tactgggcgc tgtatgcatc accggatgcg
      301 gtaataccca ggcaactgta gaaagcaccg cacaggcagg cactgaggcg cagaccaccc
      361 aggcagcgga aacagcatct accgaaagca ccgaagatgt agatcaggca gcagccgatg
      421 aagtagcagc cctgatcgac gctatctatg tacaggagag aaatgaaaat actgaccagc
      481 agtgtgcaga tgccaaggca gcatgggatg cactgaccga cgcacagaag gaactggtag
      541 agggagaaaa tgcagatccc gattatttcg gcagagatac cggagatgct tccaaggatg
      601 atccccgcaa tcaggatgag atcggtgaga acgagattct ggtagtaagc ttcggtactt
      661 cctttaacga cagccgtgtg gctgatatca agggtatcga ggatgcgatt gcagcagcca
      721 atcccgactg gtctgtaaga agagcattta ccgcacagat catcatcaat catatacagg
      781 caagagatga cgagcatatc gacaatatgg atcaggcact ggaccgcgct gtggcaaacg
      841 gtgttaagaa cctggtagta cagcctaccc atctgatgca cggggcagag tatgacgagc
      901 tgatgcaggc agtagaagcc tacaaggatc agttcgcatc cgtaaaagtc gctgagcccc
      961 tgttaggtga agtgggagac gatgcaaccg ttgtcaatgc agataagaaa gcagtagcgg
     1021 aagatctgac tgcagaagca gtgaagaccg ctgggtatga cagcctggat gctgcaaagg
     1081 aagatggcgt ggcattcgta ttcatgggtc acggaacttc tcacaccgca aaggtgtctt
     1141 acagccagat gcagacacag atgaacgaac tgggatatga aaatgtattc atcggaaccg
     1201 tagaaggtga gccggaagag accgcctgtg aagaagtcat caaggctgtt gcggaagcag
     1261 gttacaccaa agtggttctt cgtcccctga tggtagtagc aggtgaccat gccaacaacg
     1321 atatggcagg cgaggatgag gattcctggt taagccagtt caatgcttcc ggtaagttcg
     1381 acagcgtgga tacccagatc gcaggtctcg gcgggatcaa ggctatccag gatctgtatg
     1441 tagcacatac cgcagcagca atggctgaga aataaataag aattaaaata ggaattaaaa
     1501 tcaggaataa aataaggatt aaaataagta atgaaagtaa agacattaat gaataaaatc
     1561 ataatcgtgg gcggactgag tgcctgtctg ctgcttggcg gctgtggtgc cggtcaggca
     1621 gcggatgcga catctgcaga aagcacgttt gccgcacagg aaagcaccgt aacaccgaca
     1681 gagagcacgg aagcgacgga aactgtcagc acggagacgg cgcaggacga agcacagctt
     1741 cctgacggtg tctacactgc tgaattttcc acggacagct ccatgtttca tgtcagtgaa
     1801 gcctgcgatg gcaagggaac cctgacggta aaagatggcg ttatgaccat ccacatttcc
     1861 ttaggttcca agaaaatcct gaatctttat ccgggtctgg cagaagatgc ggcgaaggaa
     1921 ggtgcggtac tgctggagcc caccacggat accgtgactt acagcgacgg catgacggaa
     1981 gaagtctacg gcttcgatgt tcccgtacct gcgatcggag aggaattcga tctggcactg
     2041 atcggtacca agggaaaatg gtatgaccat aaagttgtag tatctgatcc ggtgacagag
     2101 gacagtgagg gtggcgtagc cccttttgat ctgagtactg tagaagacgg cagctacacc
     2161 atcgaactga ccatggaggg cggaagcggc agagccagca tccagtcccc cacacagatc
     2221 acggtagctg acggagcggc aacggcgact ctggaatgga gcagcccgaa ctatgactat
     2281 atgctggtga acggagaaaa atatctgccg gtaaatacgg agggcaattc cgtatttgaa
     2341 gtgccggtgg aagcactgga tgcacccctt accatgatcg gtgataccgt ggcaatgagc
     2401 acacctcatg aagtggaata caccgtaacc ttccactctg agacacttga gagtgcagag
     2461 agcggcaagt aatacatatg atgccagggt caaaaggtct aagcccacat gagcttgctc
     2521 ataggtgggt gaaagacctt aggacccacc ccgatatgag cataaaagtg ggatgataat
     2581 cccacgatat gcgaatattg ggtagggtgc tatgtgccag tggcacatgt ttagcacgga
     2641 ccgaagcgga gcggagacgt gggagtgcgt agcacggagc aatccgcagg cacaaatatt
     2701 gcaggctatg aaaggcatga aaactttgag gtcaggaaga aatagaagaa aatggatcta
     2761 tgttctgctt cttatgggac tgctggggct aagtggctgc ggcagtcctt cttctgcttc
     2821 tatggagaca tcatcggaag cagcatctga ggttacccgg acagcagaga cttcgcagga
     2881 agaacagaca aaattagtaa agacaggcgg catggagctg caatatgcgg aaaattttac
     2941 agtggattat tacgagggcg gatataagct gctgactacg ctgccggatg ataaaaggta
     3001 tcttctggtg ccggagggac agggcgttcc cgcagatctg gatgaggata tctgtgtctt
     3061 acaggagccg ttgcaaaata tctatctggt agcctccggg gtgatggaca tgtttgcctc
     3121 cgtggatgcg gtggaccaga tccgtttttc cgggcagaag caggagaact ggtacattca
     3181 gacagcaaag gatgccatgg cggcagggg
//
LOCUS       DAWEJW010000179         3185 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_7937_length_3185_cov_60.180192, whole genome shotgun sequence.
ACCESSION   DAWEJW010000179 DAWEJW010000000
VERSION     DAWEJW010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7937_length_3185_cov_60.180192"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          864 a          696 c          898 g          727 t
ORIGIN      
        1 atttctgaat agattacaat agttcaaaag aatgtctgac ggtgaaatta cggagtggtc
       61 gataaccaag ccggaagacg gacaaggagg aacgaaggat ggaattacag atattgcatg
      121 cgattcaggg gatccatcag gaatggctga cagagatcct gcgttttttt actacgatcg
      181 gagaaagcgg tctggtatgg atcgtcattg cgattgtgct gacatgcatt ccgaagaccc
      241 ggaaatgcgg tctgaccatg ataatcgcca tggcaatcac ctatcttgtg ggaaatttgt
      301 tcctgaaaaa tgtgatcgcg agaccccgcc cctgtgcggt ggataccagc gtgacattga
      361 aaattccgtt cccttcggag tattccttcc cttcgggaca tacttccaat ggttttgccg
      421 gagcggtgac cattttcagt tattatcgta aggcgggtat tttaagtctg cttatggcag
      481 cagtcattgc cttttccaga ctttatttct ttgtccacta tcccacggat attctgggcg
      541 gcattgtgct gggaacatta gatgcgctgt tggcggtcta tctggtaaag cgtctggaag
      601 accgggcgga tgcaatataa gagcagaaaa cggacaggaa gaggtaggga cagaaagggt
      661 agaaatttcg atgaatagca gaacattatg gaatgataac tggtattttg ccaaaacagc
      721 attggatgtg gaatgggaag accgggcgct ctgggagcgg gagatgcagc cggcagactt
      781 gcctcacgac tggctgatct atgatacgaa gaacctgtac gaagacagca tcggctggta
      841 tcggaagaaa tttgtgtatt ccacgtccgg aacaaagtgt ggcaaacgtg tcattttgcg
      901 cttcgagggt gtttacatgg attcttccat ctatataaac ggcgtgtgcc tcggtgactg
      961 gaaatacggc tactccacct ttgactggga cattacggat catctgcgtg aaggtgaaaa
     1021 tgaagttgtt ctccgggtaa cctttcaggc acccaacagc agatggtaca gcggtgccgg
     1081 aatctatcgt aatgtctgga tgatccgtct gcctgagaca catatcccgt tggacggcat
     1141 ctacacctct tctgtggaga cgcaaaaagg ctatgacctg accatcgata tggagttgaa
     1201 atggggaacg gacaacggaa attatgcact ggaatattgt ctgtattata caggggacag
     1261 taaaagctcc gcagaaacag caagtaggga gattttccgg gtaaagcagc cccttgacgg
     1321 tggacaggag aagacatctg tgacgattcc tgtggacaat ccgaggaaat gggacattac
     1381 ggatccttac ttgtatgagc tgcaggtatg cctgtggaga gacatgttgc gggaaaacgg
     1441 tagtgcggag acgatcggta acagtgcatc acagagaaca gaacaacaac cggtgcatga
     1501 gaccaacagc cgggaactat gtcaggaaga acatcttcgc atcggtttcc gcaccatgca
     1561 atttgatcct gtcaggggct ttttattaaa cggcagaaaa gtgcgcctga acggtgcctg
     1621 cgatcaccat gacctcggag cactgggagc ggcttttcac aaagaagcca tgcgccggaa
     1681 atttgaactc atgcgttcca tgggtgtcaa tgctgtgcgg acttcccaca atatgccctc
     1741 tgtggaactg atggaactgg cggatgagat gggatttctg atcgtatccg aagcgttcga
     1801 tatgtgggag atggcaaaga atccttatga ttatgccaga tttttcaagg aatggatgga
     1861 gaaggatgta cgcagctgga tccgcagaga ccgcaatcat ccttccgtta tcatgtggag
     1921 cattggcaac gagattccgg atacccatgc ggatgcccat ggacaggaga tcacccgtag
     1981 cctccttgca gtagtcagaa agtgggatta ccggaaggtg gcaccggtga cgatcggttc
     2041 caattacatg ccctgggaaa atgcccagaa atgtgcggat atcgtgaaag tggcgggcta
     2101 taactacgcg gaaaaatatt atgcggaaca ccatgaaaag catccggact ggatcatcta
     2161 cggaagtgag acttcttctg tggtccagag ccgcggaata tatcattttc ccctggagcg
     2221 ggcgaccttg atcgaagcgg atgagcagtg ctccgcactg ggtaacagta ccacaagctg
     2281 ggcagcaaaa aatacggaat attgtattat tatggacaga gatactccct attcctgcgg
     2341 ccagttcatc tggacggcta ttgactatat cggtgagccg actccctatc agacgaaaaa
     2401 cagctatttc ggacagatgg acacggcggg atttcccaaa gattcttatt acgttttccg
     2461 ttcggaatgg acggatggga aaaaagatcc catgatccat gtatatccct attgggattt
     2521 taacccggga cagcaggtgg atgtgcggat caccagtaat gccccggagg tggaactatt
     2581 cgtaaacgga gtgtcgcagg gaaaacagca gatcgaccat cagaagggaa cagtattaca
     2641 gccttcctgg aaagtaccgt atgtacccgg cagtatctgt gccgtggcat acgacgaaac
     2701 gggtagggag atagccagac aggagagaca ttccttcggc aacacagatc attatgtatt
     2761 aaaagcaaat aaatcagcct tgcatgctga cggcgaggac atgatctttg tggagatctc
     2821 agcggaggac cgggacggat atcctgtgga aaatgcctcc gattatgtga gagtgaccgt
     2881 ggaaggcgcc ggacgtctca taggcttgga caatggtgac agtaccgatt acgatgccta
     2941 caagggaact gtccgtaagc tgttccaggg gaaactactg gcgatgatcg cagcgaaaac
     3001 gattcccgga gagattcgtg tgaccgtgga ggacgcatgg acagccacgg tgagcatgac
     3061 cgatgaaacc ggtgccgaaa cggccacggc agtagtcgag actccggaca atacggcagc
     3121 tgccgggcat cgcacggcga cagtgacgtt acaggcagtc gaagcaccga ttcgtcccgg
     3181 tgtct
//
LOCUS       DAWEJW010000180         3179 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_7956_length_3179_cov_65.754802, whole genome shotgun sequence.
ACCESSION   DAWEJW010000180 DAWEJW010000000
VERSION     DAWEJW010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7956_length_3179_cov_65.754802"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          752 a          798 c          736 g          893 t
ORIGIN      
        1 tcacccacct atgagcaagc tcatgtgggc ttagaccttt tgaccctggc atcatgatta
       61 tattacaata gagaatataa ccggcagcat cgaaagcttt ctcaacataa aaccctggac
      121 acagttttgg catagactct gaaccaagcc agtataagct gatgaatttt tacattctac
      181 aatttaaata tttaccattt ttacaaagcg ggtaattgtc aatgaatatg agtaaaatcg
      241 atatgacaca aggttctatt ttaggccgcg cagcacagtt tgcattgccg atctgcatcg
      301 gaaatatctt acaacagtta tacggaaccg ttgatacttt ggtcatcggt aatttctgtg
      361 atgcatccgc tttggccgct gtggccacaa gttcccagcc actggagatt cttttgtgta
      421 tctttcttgg cattggttcc ggaatatcca tcctgatttc acaagcggca ggtgcctccg
      481 atgccgatca actgcgtaag cttgcacaaa ccgcagtgtt ttttctgtac gctgccagtc
      541 tgccgcttac gttgttaggc gtagctgccg gaccgttcct tctcaggctg atgcaggtgc
      601 ctgccgatgc catgccctac gccattgtgt atctgcgtat cacaacgtta ggcattctcg
      661 gcaatatggg atataacttt aacgcaggta ttttacgagg tctgggaaac agtacttcct
      721 cgctgttatt tcttggcatt tcctgcgtta ccaatattgt tctggacctt gtactgactg
      781 ccggtttggg aatgggcgtc accggtgttg ccatcgcaac tgctgcctca ctgtttctgt
      841 cctggatgtt cagcatcttc tacatccgtc atacttatcc tgccatgaat tttccggttt
      901 ttgcgcatgg ttttgacctt cctattcttc atgaaatgtt aaagatcggc ttgccgctcg
      961 gactgaatac cgcactttat tctgtcggtc atgtgctgct tcaggctctt tataacacac
     1021 tgggatccac cttcgtagcc ggatgctccg ttgccggtaa agtcagcggc attgccaata
     1081 ttgccattac ttcatattct tctgcggcta ctgtgtttgc cgggcagaac cttggtgccc
     1141 ggaactaccg tcgtttatgc tgcggtgcat ggcagatacc gctctgttcc ggcatcgtca
     1201 cccttgcagg gggattactt gtgactgctt tttgccatcc tctgctgatg ttgttcagcc
     1261 gtgatccggc cgtgttggct tttgcacagc gatatactta tcttgttctg ccattcacct
     1321 gggcctacgc cgtgttcaat ggcattatgt gctttatcaa tggtatcgga gagattcgct
     1381 atcccactat cgtaaatatt ctgctattat gggttgtccg aatccctgcc gcttatctca
     1441 tcatgtatgc cggctatggc gaatatgcca tggtcagtgt ttccgccagc tttattaccg
     1501 gcatgattgc catgctcttc tactttaaat ccaggcgctg gggcgctatt tgcgcccttg
     1561 caaaaaacag gaatacccac cattaattac tgacttgcca gattcataaa aatttacata
     1621 cttcctaaaa ataatcacat ggattttcct tttgtcctgc cctataataa gcgcaattac
     1681 agtccacaag gcaaaaggag gactaccatg ttatatccaa ttatgaaccg ttcccgcagc
     1741 ttgatcgatc tgggagggat atggcacttc caattaggag atgagacgga accggaagat
     1801 acctggtcag ctccacagga gatggaattg atcgctgtgc cggcttctta taatgaccag
     1861 aaggatgatc cgtcttatcg gaatcattac ggttgggctt attacgaacg aaccatggaa
     1921 cttccggcac ttttgtgcgg aagcaacgga cagcgtcagg tactgcgttt tgatgctgta
     1981 actcatgttg caaaagttta tatcaatggg aaactgatta tgacacataa gggtggtttt
     2041 ctcccctttg aagttgagat cacggatctg gttccggagg atggccacct taccatcggt
     2101 gtcgctgtgg acaaccggat cagccacagc actctgccca tcggcaatga gggcaatatc
     2161 gccttcttcg gctcggacaa tccaggcatt cccagtgtag aagccgcaaa gctatggaga
     2221 aaaaagcaaa atcttccgaa ttttgatttc tttaattttg caggtatcaa ccgtccggta
     2281 cgcatctaca gcactccggc ctcctacatc agagacatta ctctggtccc gtctatcgaa
     2341 ggtgacaccg gcgtcttaaa ttacgaaatt ctcacggatg ggatggatag tgacgctcct
     2401 gtcactttaa aaatcataga cgcggaaagc aactgcaaag cagaagccac cggccgaaag
     2461 ggagagcttc ggatcccgca ggtccatctc tggcagcctt atcccggcac tccttacctg
     2521 tacaaggcgg aagttcatta cggagaggac tgctatacgg aagaattcgg tttccgcacc
     2581 gtagaggtga agggaacgca gtttctgatc aacggggagc ctttttattt caagggcttc
     2641 agcaagcatg aggattcctt tttctgcgga agaggactgg atctctgcct cgatgtaaag
     2701 gatattaatc ttctacactg gatccatgcc aactccttcc gcaccagcca ttacccttac
     2761 gcggaggaga tctaccggct ctgtgacagg gaaggtatcg ttattattga cgagacgccc
     2821 gccgtgggca tcggtgccgg agagagctgt gatccttata agacatttcc cataagagag
     2881 catcacgagc aggtcctgcg ggatctgatc gcgcgagaca aaaatcatcc ctgcatcgtc
     2941 atgtggtcca tgggaaacga accggatacc gagcatttcc cggaatccgc ctacgattac
     3001 tggcattctc tctatgaatt tacccaccgg ctggatcctc agaaccgccc cgtgaccttt
     3061 gtctgctgcc agaataatta cgagaaggac attgtgacca gaaccatgga tgtagtctgt
     3121 ctcaaccgct attacggatg gtacaatctc tccggtgatc tggaggctgc ttcctacgc
//
LOCUS       DAWEJW010000181         3138 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8057_length_3138_cov_69.592280, whole genome shotgun sequence.
ACCESSION   DAWEJW010000181 DAWEJW010000000
VERSION     DAWEJW010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8057_length_3138_cov_69.592280"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          849 a          651 c          839 g          799 t
ORIGIN      
        1 ttttttatgc aaaaagcttc tgtaaaaagc atgtaaagaa agttgaacaa atgtaagcgg
       61 ttacataata ccaaagtact agacaaaaca aacaaaaaca caatagctat tttcaacatt
      121 atgcacgaaa attggtagtc tgtcaaaaaa ttatcaattt cggcttgata ttatattggt
      181 agattgttac aatatcaaag tactaaaaac cgaggccgaa atgctccggt aatggtttgg
      241 gttacaaagt tttgtcaaaa atattataaa aagggagaca aagaagtatg aagaaatggg
      301 tatatttgtt taccgaaggt aatgcaaaca tgcgcgaact gcttggtggt aagggtgcga
      361 acctggctga gatgaccaat attggtctgc cggttcctca gggcttcacc attacaacag
      421 aggcatgtac acagtattat gaagatggac gcgagatcaa tgaagagatc cagggtcaga
      481 tcaacgagta cattactaag atggaagaga tcaccggcaa aaagttcggc gaccacgaga
      541 atcctttgtt agtatccgtt cgttccggcg cgagagcatc catgcccggt atgatggata
      601 ccattctgaa ccttggtctg aatgagaccg ttgttaacgt aatcgctgag aagtccggca
      661 accctcgttg ggcttgggac tgctatagaa gatttatcca gatgtattcc gacgttgtta
      721 tggaagtagg taagaagtac ttcgagcagc tgatcgatgc aatgaaagag aagaagggtg
      781 ttaagcagga cgttgagctt actgctgatg atctgaagga gctggctggc cagttcaagg
      841 ctgagtataa ggctaagatc ggtgtagact tccctgatga tcccaaggaa cagctgatgg
      901 gcgctatcaa agctgtattc cgttcctggg acaacccgcg tgcaaacgtt tatcgtcgtg
      961 acaacgatat cccttattcc tggggtactg ctgttaacgt tcagtctatg gcattcggta
     1021 acatgggtga tgactgtggt accggtgttg catttaccag agatccggct accggtgcta
     1081 agggcctgtt cggtgagttc ctgaccaacg cacagggtga ggacgtagta gcaggtgttc
     1141 gtacacctat gaagatcgct gagatggctg acaaattccc tgaggcattt gctcagttcc
     1201 aggaagtatg taagactctg gaagatcatt acagagatat gcaggatatg gagttcactg
     1261 ttgagcatgg taagctgtac atgttacaga ccagaaatgg taagagaacc gctcaggcag
     1321 ctctgaagat cgcttgtgat ctggtagatg agggcatgag aaccgaggaa gaggcagttg
     1381 caatgatcga tcctcgtaac ctggatactc tgttacatcc tcagttcgat accgctgctc
     1441 tgaaggctgc aactcctatc ggcaagggtc ttggtgcttc tcccggagct gcatgtggta
     1501 agatcgtatt caccgcagaa gatgcagaag aatggcatgc aagaggagag agagttgttc
     1561 tggttcgtct ggagacctct cctgaagata tcaccggtat gaaggcttcc cagggtatcc
     1621 tgaccgttcg tggtggtatg acctctcacg cagcagtagt tgctcgtggt atgggtacct
     1681 gctgtgtatc cggttgtggc gatatcgcta tggatgagga gaacaagcag ttcactctgg
     1741 ctggcaagac cttccatgaa ggtgactggc tgtccatcga tggtactacc ggtaacatct
     1801 atgatggcga gatcaagact gttgatgcta ccatcgccgg tgagttcggc cgtgtaatgg
     1861 cttgggctga caaatatcgt aagttaaaag ttcgtaccaa tgcagatact cctgcagacg
     1921 ctaagaaggc tcgtgagctg ggcgctgaag gaatcggtct gtgccgtact gagcatatgt
     1981 tcttcgaaga ggacagaatc gcagcattcc gtgagatgat ctgttccgac actgtagagg
     2041 agagagaagc agcactggag aagattctgc cttatcagca gaacgacttc aagcagctgt
     2101 atgaggcttt ggagggcaac cctgttacca tccgtttcct agatccgcct ctgcatgagt
     2161 tcgttcctac cgaggaagct gacattgaga agctggctaa ggcacagggc aagagcgttg
     2221 agacgatcaa gaccatcatc gcttccctgc atgagttcaa ccctatgatg ggtcacagag
     2281 gatgccgtct ggctgttact tatcctgaaa ttgccaagat gcagaccaag gctgttatcc
     2341 gtgcggcaat cgaggttaag aaggcacatc ccgactggaa tgtagagccc gagatcatga
     2401 ttcctctggt atgtgaagtg aaggagctta agtacgttaa gaagatcgta gtagagaccg
     2461 ctgacgctga gatcgcagca gcaggcatcg acatgaagta cgaagtaggt accatgatcg
     2521 agatccccag agcagctctg actgctgatg agatcgctaa ggaagccgat ttcttctgct
     2581 tcggtaccaa cgatctgacc cagatgacct tcggcttctc ccgtgatgat gcaggtaagt
     2641 tcctgaacgc ttactatgac accaagatct tcgagaacga tccgttcgct aagctggatc
     2701 agaccggtgt cggcaagctg atggagatgg ctatcaagct tggtaagcct gtaaatccta
     2761 agctgcacgt tggtatctgt ggtgagcacg gtggagatcc ttcttccgta gagttctgcc
     2821 acaagatcgg tctggattat gtgtcctgct cacctttccg cgtacctatc gcaagactgg
     2881 ctgcagcaca ggctgctatc gccgataagt aatttggcgg taaacacgca gatctgagag
     2941 ggtattgcgc aagcaataaa ttggggtata tgagaataag atgagaacct cattactgat
     3001 agaaatatca gtaatgaggt tcttttttgt tactatttcg tctgaattaa agcacgatat
     3061 ttccgtcccg gtcgattgat gtcgtttttg ggtcgattgg tgcaatctgc cgcttatttt
     3121 ctggaaatgc tgttataa
//
LOCUS       DAWEJW010000182         3119 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8108_length_3119_cov_53.340731, whole genome shotgun sequence.
ACCESSION   DAWEJW010000182 DAWEJW010000000
VERSION     DAWEJW010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8108_length_3119_cov_53.340731"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          966 a          720 c          664 g          769 t
ORIGIN      
        1 tgtttattgt ccaaacttaa tgcaaagcaa catatcgagg tagacatcca catggacgaa
       61 cttgatctca ccgatgcaga gaagaaagcc acctattcgg aaatcaagga atatgtgctg
      121 gagcataccg gactcaaggt cagcagcttg tatatcgctc aggtgaagca gaaatgcggt
      181 atcatcgagc gagaaaatta caacaaaccg aagtccgatg atgcaaaaca gccccaatgc
      241 ccgcccgata aggaaaaagc aatcaaggag gcgctcaagc acttcggcat gatctaagga
      301 gggcaaaatg aagcgtttac tttcttgtga gttcaatatg gatactgcct gtgtggagct
      361 gaaatttgca aatggcagca tgattgccat tgatacgatt gcggtagaga atgaggttgc
      421 cgataatata tatcagcggt cagagctgga ctggctgatt tacaacgatc cggcagccta
      481 tgcagattta gttctcaacg gcgacccgga aacatacctg aaaactgtta cagaatataa
      541 acctttggac tgattaccag agataaagca agccgcctgt gcgggcatat caaatgctca
      601 tacaggcggc ttttcctttt tgtactgtga ctttatcagt tctccgaaat ggaccgctta
      661 aacaatatcc atgtacttat gaagtagcat atcagcagta agaaaagaat accaatgata
      721 atccctactt gtaccatcaa ggctccgcag ccaatataag ctgagatttc agcagaaaca
      781 ctttgaagaa aatagccaat cacaataatt gctacaacga ttgccgctgt gatcggcatc
      841 ccaaaccaaa caccaagctg ttttagaacc aaagtatgca ggtccttctc ttccactccc
      901 atttttcgca gaaccgaaaa tctgtagttg tttttttctg catccaggag ctgctgaaga
      961 gccagcacgg taagacacat caccatcaga acaattgcac catagatcaa ggacgctttt
     1021 aggataaagt taagggcgat tatacggttc acttccgtag tatggacagt tgtactgtat
     1081 cctgccagat tgtctttatc aggatcttct ggataagaac gcccaagaag ctgttccagc
     1141 atttctgccg ttttgaaggg aagcggatat tgggtcatca caaatcggtt gcgctgtacc
     1201 ggaagcaaga cttgcgctat ttcgtcaggt atgatataca ccacatctgt ataaagatta
     1261 tagatggatt cccccacagg ctcctgaaac acggcgttct cactgagttt cagggtgcct
     1321 gcgtcagttt ccaataaggt atgctcagca atgtagtttt caatatcctt gtcctctgct
     1381 gcacgatgcc agtgggtagc aaattcatcc gtttgtaaga taataggttc atatcccaac
     1441 attttccgaa cagcgttata atccttcagt gcgatcgcca agggaggaaa attgtacttt
     1501 actcgctggt gaaagtcgct ttgcttggga agatattccg aaaaagtcaa atcgtccttt
     1561 atcgctattt tgttttgttc tatgaacgca gtaatttctc catagtctgt atccggaaga
     1621 ttttccactt cataaacatc gttgtagcgg cttgaaattt gaatatcata gacagcccga
     1681 ctgtccaaat agcccagact ccatcccgtt aaaaccggcg caatgacaaa caagcagata
     1741 gaaaaagtca gggtcacaca aatgatcgtc atcgttttcg tattggtagc cagcttagag
     1801 gaaagctgtc caaacagaaa taaactcgta ttatgatatt tgcgggacac tttagactcc
     1861 ttccagtata ggagcgccgc attcgataaa tacatgactg cggaaatgat aaacaaaata
     1921 tccgcaaccc caaacagcaa atattgattc agagttggag cgtcaaatcc aaggaagtag
     1981 gctttttcca gttctgcgat ggaaaaaatc gggatagcgg tcaatacagc accagccaga
     2041 agtccgcaaa gatatcggga aaaccctatt tttctgtgta aaacagcacc tgcgattgcc
     2101 caaagcagcg tcagtgcagg gaaaaggaca ttcccccaat acatgagctt tacaggaagc
     2161 ggatgtctgg aatcgaagta aaaatgatat tttacagtac ctacctccag catccacagc
     2221 agcagaatgc catagaaaat acagatcatc ggcatccatc tgcttttatg aagcggtttt
     2281 tcattctgac ggtttgccgt cataagctca ataatcctgc ttttgttgag aattctgaca
     2341 ttcccgactc caaccagaat ctggcaaaca acaaagaagc ccacggtcaa cagcacagta
     2401 tccgggaaaa acgaccaaga aaaagcgtag ggttccccaa aagaagcaag cagcatagcc
     2461 gtaatgacct gagaaacgac catccccaga aggattccca ccgacacaga gaatatgagc
     2521 aggaaaaaag tctctccaaa aaagagcagg cctatggttt tttgctccat tcccaaggtt
     2581 gcctcaacgg caaactcctt ttgctttttt ctcagcatga aacgattgac atagtggatc
     2641 agaaacaaca aaagcaaact aatcccgcaa attgccagtt tcatacctcc agccagcagg
     2701 gaaatattat attccgcacc aatggtcgga tgataatggg tgctgctgat agacaaaaac
     2761 gcatagaaca gggtgacaca gagtgtcatg gtcacaatat aaatcaaata gtccttcaca
     2821 gaccgctttg catttttgaa aataagttta gcgtacatcg ctcatgcccc ctcccatcat
     2881 ggtaaggaca tccaggattt tttcaaagaa agtcctgcgg gaatcacccc ccttacggat
     2941 ttccgtaaag attgccccgt ccctcataaa gagaatacga ttggcatagc ttgccgagaa
     3001 agcatcgtgt gtcaccatca gaatcgtggc tccgagatct tcattgatac tctggatcgt
     3061 cgagagcagc atctgggacg agtgactgtc taacgcaccg gtgggttcat ccgccaaaa
//
LOCUS       DAWEJW010000183         3113 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8124_length_3113_cov_54.096795, whole genome shotgun sequence.
ACCESSION   DAWEJW010000183 DAWEJW010000000
VERSION     DAWEJW010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8124_length_3113_cov_54.096795"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          750 a          765 c          741 g          857 t
ORIGIN      
        1 gtgggataca tgtgtccgtt gtattcgctg atcaggtaag gcttgtcggt atcggaagtg
       61 acctttttct tcgggtcaca gccgggagtc tcgccgtcgt gcagaaaatc gttataagta
      121 tagacgtcct ccaacagatg gcttttctta atagcccgga caccgccggt aggtctcgtg
      181 ggatccagct tatgtgccac ggcattggtc ttttcgtaaa acggatcatc atccggggat
      241 tcattgatcc gtacacccca caaaataatc gaaggatgat tgcggtactg acggatcatg
      301 tcctctgcat tgaccaccgc ctgtgctttc caggtgtcat ctccaatatg ctgccagccg
      361 ggaaactctg tgaagaccag aagtcccagt tcgtcgcacc gctccagaaa ataatgactc
      421 tggggatagt gggaagtccg cacggcattc agccccagct ctttttttaa cagatccgca
      481 tccagttttt gcatgctctc cggcatggca tagcctacat aaggataact ctgatggcgg
      541 ttcagacccc ggatccgcag ttttttgcca ttgaggtaaa agccgtcttt tttaaaaact
      601 gcatccctga taccgaaggt gacctctgct tcgtccagaa gctgctctcc ctgccagagc
      661 tgggtcttta acagataaag atgagggtgt tcggtatccc acaaaaaagg atgtggaatc
      721 gttccctgaa tacgaaacgg ggtctcagca gtagtgcctg tggggacatt ctgttcgcag
      781 agaaactgcc agtcattatc tgatgttaca tctgatacca cacccgacat tacagcatcg
      841 ctctttaaca tataatattg tctgacatta aggtctttcg caacttcata aaaagtgatt
      901 tcagaagtta cctgagcctc atccggtgtg atcagcgtgt gtacgaaaat atcctcaagg
      961 gcaatctggt cttttacttc gagatacaca tcccggtaga tgccgccgta ggtcatataa
     1021 tcgatcacgt aaccgaaggg aggcacattt aagtcttctc tgctgtccag ccggacacac
     1081 agcacattgt cctgtccgta gttcgcatat gccaacaaat ctacagtaaa ggcggtgtag
     1141 ccgcaatggt gctctcccac cttgctgccg ttgagataga cggtactgtc atgaccggcc
     1201 ccttcgaagg tgagcaggat ccgcttgccc tgccattcgg tcggaatatg cagatggcgt
     1261 ctgtagccgc tgaccatctg gtagagagat tcgtcaaaat agtgaaacgg ggtctcctta
     1321 caggtatgag gaattcggac gggctgcagg gtctcttccg ggtattctgt tgcagtcatc
     1381 ccctcctgaa agttctcgtt aaaaagccag tcttcgctta aatagatctt ctgattcatg
     1441 gttcgttcct ctttctttgg gatattatgg attaattctg gattattcca ttgcctcatt
     1501 ctgatctctg tacgatgcca tacatgagga tttccacaac ttccttcagg gcatctgcat
     1561 aagtcagctg atttcgagag agaatatctc tctggaagaa ctgctcaatg gaagcttcta
     1621 acatcatttt caccagacag atgggaaccg ggcggatgct gccctcagcc atgcccatct
     1681 cgatcagctg gattgtaggc tcccagccag tggaaagtct gagttctacc ttctggtaga
     1741 tcttcgggta tttgtcccgg agggaatata attttcgaag atcgatattc tgataacctt
     1801 ccggcagcac tcccaggatg cggtgcagtt tttccactac actgagggaa ggatcggtca
     1861 ggatcatctg ctcgctttcc ttgatggagt caaagatata atctaccatg gcaagaaaca
     1921 gtgcttcttt atcatcgtac acggtataga tggttttctt gctgatcgag agttccttcg
     1981 ccagatcatc cattgtgaat ttcagccctt tttgatcgaa gacacgcagg gtggcgtcga
     2041 gaatattttt ttgcagacct tctgtggtat ccatcgcttc tattcctccg ttcggtaaga
     2101 ttgtaattgc agtatggaaa ctgaaaagta aattgtggtt tcctataaaa acagggtagc
     2161 atttttgaaa aaaagattca agaaacgttt ttgccaaaaa gagtttccga gaattgtgaa
     2221 atataacaaa aaaccagcca acaaaacggt tgactggtga atgacagcgt ctataaatag
     2281 tttttcataa tgcggtacaa tgcttttgga tgcagcggtt tggcttccgt tggaaaaggc
     2341 ctccactacc gctccggcgg cgtggagaag tattgttctg ccagcgtggg atgagatttt
     2401 ttgcgggtga tctccaccaa tcctaaaggc gtcatatcca cgatccgggg atgctgggga
     2461 tcccctgcgg tcagattctt taatgtctcc agtaactcct tctgccgttc cttttccgcc
     2521 atattgatga agtccacaat gatgataccg gacagattcc gcaggcgcag ctgtctggct
     2581 acttcttcgg cagcctccag gtttacctga taataggtgt cctctcctgc ctttttcgct
     2641 tcgtttttac cggaattgac atcgatcacc gtcatggcct ccgtaggctc gatgacgaga
     2701 taccctccgc attttaacca gactctcgta tccagtgctt cctccatgcc gcgctccaga
     2761 gaatacagca gacgtaaaga gatcgccgga tcatcgtaga accgaatgga cttttgtttt
     2821 aacaaagggt gatctccttc cgacagctga cgataggtct gaggatcatc cgtaaggatc
     2881 tcagaatatt ccgaaggatc cgccatctgc tccaaagcct cgtaaacggc ctcagaaggc
     2941 ttcaaaaggc aggcaaagta cactttatgg tctgcctgct ccaaaaggct ctgaagggct
     3001 tcagaagcgt tctcaggggt gctcccgttt ttacaaagca gctgcttcat ccgggcagga
     3061 tccgcagtgg cggaggcacg ctttgtcttc tgggcatctc tggtgaccat gac
//
LOCUS       DAWEJW010000184         3098 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8175_length_3098_cov_89.859349, whole genome shotgun sequence.
ACCESSION   DAWEJW010000184 DAWEJW010000000
VERSION     DAWEJW010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8175_length_3098_cov_89.859349"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          747 a          743 c          545 g         1063 t
ORIGIN      
        1 ctcaaagatt gattgtacca tttgtttttc ctccaaataa gatattcact ccatactcat
       61 gtctgctatc attaacacaa tttacgtttt ctccttatct ggtttcatac atgtattgta
      121 atcctacaat atcactcttt aaaaaataaa agacttgttt tgaattattt tttatagtat
      181 cctataatag tggtgattta tataatttta atgtttattt ttaagattct ctaacaaact
      241 tttatactat cctataaaac ggtcaatttt aaaaatcacg aaattgcagg ctttacaaca
      301 ctttgtaaca cgatataggt ctgtccgata atccctctca tataaaaata atcaatgctt
      361 catttttaac acaggaagat aaagacatct ccctgtgttt ttcttaataa tatccccgct
      421 cttctcgcca gtcttttaca taacatcgaa ttccaatata gaccacatat acaagtagta
      481 acagtacaat gaggtttatc ggaatgacag attttatcca ttcgccaaag tagattacga
      541 tagctgtact cattattgcc accataatgc tgatgatatc ccaacaatat ttccggtaga
      601 tttttcccac ctgatagcca atcccaatta tcaggaatcc cgttataatg aataaaattc
      661 ccataacaat agccgcgatc agccagtaca caatcccgga aacgatactg tttgagattc
      721 cattgcttaa ctgtccgaaa gaatctgctc ctgtaacaaa ttctcgaaac aggtttatga
      781 ttcctttacc aatcgtaccg aaaaaggtga tacagtcgtt aagaaaaacc ggagaaagga
      841 tagctgtgaa cagtgtagtt gctatgctgt accatgccaa caggaacata aggctttcat
      901 aacctgttgt catttgttta tattttttct ccaactgaat ttttcgatta tcgcattttt
      961 cttttgcttt ctgataagca gtacggtcac agttatcgca tttctcataa ggcacttttt
     1021 tctcgacagg cacctctacc gtcttttggt gtgtctgtgc atagcgtttt gcctgctccg
     1081 ctttttcgta cttaaatttc catgccacga cttctgcgtc ggctcttttc ctgctgtctg
     1141 tctcgctggt taattgttcc tctgtttgct ttaattttgt ccgcaatgct tcgatattct
     1201 cggctctgtt ttcgctgttc aatttctcgt tcaaggtcag cgatctgttt agctgcctgt
     1261 tcagttccag aattgtctgg tctttctgat ccagttcgtc ctgcatctgt tccgtcattg
     1321 ccaggagttc ttccacccgc ccgacgttct ctaagtttac gcattcgttc atcaatatcc
     1381 ctcgctttct ctatctgctg tttaatgtca gtaataagct gttctcgttg ttcagcttct
     1441 gcgttaattc ctgccattgc tgattctcgt tttccaactg ttcgattctg actttttgct
     1501 gttcgatctg ggagagcagt tcttctgtat ctggcaaaag attcagaagc ccggataact
     1561 cgctgtttaa gatctgcaaa ctctggttct gctcctgaaa ggacagaagc tgtctgtccc
     1621 gttcctgtaa ttcctgtacc atctctgcca taaggttctg ctgttcctcg atctggttta
     1681 tcattgactc catcatggga atgacttccc ggctttcttc taaggtcatt taaccactcc
     1741 ttccactgtg ataatgcacc agatactttt ccgaaactgg tctgtatctt tttcagcagt
     1801 gcattttgtc tttttatgat ctggttttcc tctactttcc atgaagctgt ctgcacctgt
     1861 ccactctgga atttctcatc aatctttctt gcatcagcac cctcatggat tgtagggatt
     1921 tctaattttc cctgtcttgc ataggaacgg tggtcaatct gttgttctac tgataaatgg
     1981 tcattacaga ctttcgccca ttcgctccgc cataattcac aattctttgg attgttccat
     2041 ccggtagcat cggtcagcac ccgtttccat tgtttacggt tccttgcacc aactttctga
     2101 ttaccgtttt cgtccaatac cggaattcgg attccatgac ggtcaggatt ctttttatcc
     2161 tgccaccagt ccgggtggga ttcatcaacc acaatatttc cgtcagtatc tctgacaaac
     2221 tcccagtctt tgacttcttt gtttccccat gagtggtcgg gattaaaggg acgcatggtt
     2281 acaagtaaat gcacatgcgg gtttccgtcg cccttatcgt gaatgctcca atctgcacac
     2341 atgcctttgt ccacaaagtt tttctgaata taatctgttg tatattcaat ttgttcctgt
     2401 cgattccatt cttttggaag tgagaactca aacgatctgc caagctgtgc gtctgaactt
     2461 ttctcaatct tcaaaacttc attccataag cactgtttct gaaaagtaag tttgaatttc
     2521 tccagtacaa cttctttgtt gtctgccctt ttataccgga cagatttctg aaaccgtttg
     2581 atattttcgg caggtacatt ctgccattcc tgcggtgcat tttcacacat catcaaactg
     2641 gtataaacaa cttctctttt ggaagtgtag taactgatcc tgcctgtttc ttcatttttc
     2701 attacatcac cattgagata ggcagacgct gatatgatgg atttcccttt gctgcgtccg
     2761 acaatcttta ccgtataatg aaagatcgcc atgatccgtt tccccccttt ctgccgtacc
     2821 cggcattgat ttccggcagt taacatttcc tgtaaaagag aatggtatct gccggaacgc
     2881 cctctgcgta agagtgctct gtgcataaca gcctgcatgg aatgcgcccc tctggcgaag
     2941 agtgcctcct gcgacatgag caggtctgca tgaaatgccc gccgacggaa cgagcccggc
     3001 aagcgttagc gcagaccggt tgccacctgt ggcaaactta tacggacgac ctctggttgt
     3061 ccataagtgc gcccttcata aaaaagcaat gaagggaa
//
LOCUS       DAWEJW010000185         3040 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8380_length_3040_cov_69.270687, whole genome shotgun sequence.
ACCESSION   DAWEJW010000185 DAWEJW010000000
VERSION     DAWEJW010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8380_length_3040_cov_69.270687"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          692 a          815 c          701 g          832 t
ORIGIN      
        1 cggtccacta ccgcttcctt ccactccggc agggaaccgg gcacattcca ggaaggttcc
       61 acagctccca tcttctgcca ggagccgtga ctctccagat tcgtctccgc catcatgtaa
      121 ataccggctt catcgcacaa tgtgtaccat tccatgcgat ccggataatg acaggtacgg
      181 acggcattga tatggttacg tttgaagcat tcgatgtccc atttcatatc ttccagggaa
      241 atacaccgtc cggaagctgc attccactca tggcggttca ctccacagat caccagccgc
      301 tcgccgttca gcttcattac cttatcctga ggatccagcg caaaatcacg gaagcctatt
      361 ttatatggca ccacttccgt cacttccccc tcactgtctg tcagagtgat ctccaactga
      421 tataagtacg gattctcatg gctccagggg attacctttt ctgccaatgt ttcccgaagg
      481 tgcagcgtct gctccgcctg tgactgcaaa atctgtgtct gctccaacag gcatttcccg
      541 ttggcatccg acagacatac acgcattttg cccggacgct gtccctcacc ctcagcagat
      601 attttgattg ctgcatctaa cgtgcctttt ccatcctcta cggaaaaata aggcttcgcc
      661 caaaggtctt ccacgtgcca ctgcggcttg gcatataatt ccacactccg gaagattccg
      721 aaaaagcgga agaaatcctg atcctccaga aaagcagccg tactgcgctt atgcacttcc
      781 accgccagca gattgtcctt ttccttaata taaggtgtca gatcgaattc agagggagta
      841 aagctgtcct cggcatatcc cacgaaatgc ccgttcagcc agacatacat cgcctgttcc
      901 acaccttcaa aacagataat gactctcttt ccccgcagac cttctgccag atgaaaacgc
      961 ttccggtaag aacctacggg attatactct gcctcgctga aagcaccgtc tgccaatcct
     1021 tttcccagcg tgtaagccgg gcggcggtaa attttgccct cccagggata catggtgttg
     1081 atataatgaa tttggtcata acctgccagt tcaatatgtc ctggcacacg gatcgtatcg
     1141 aactgcgcac tgtcagcatt ttcccggtag aaatccaccg gacggctctt cgcattcacc
     1201 gaataggcga actcccatat tccgtccaga gactgtacca gggagcttct gccctctgcc
     1261 gcttcttccg tatcctgata gatcggatga tcactgtggg ctggcagctg accgacccgg
     1321 aacaccttcg gatcgtccag ccatctgata tctgcttcca ttttggtcct ccttatttat
     1381 cccttttcta ttataataat tgtgaatttt cttccatgca acaaatattt atctcttcgg
     1441 aaaaacagat tccatgatac actgcccgga aatcaagatt tttgtggcag catatgtgaa
     1501 aatatgttaa tctgtaaaag tatttgcctt atatccacaa atgataggag attctgttta
     1561 tgttatccac aattgaagca gtcaacagtg ttgttaacaa tttcatctgg ggtgttcccg
     1621 ccatgatctg tatcatcggc gtcggtctgt atttaagtat ccggacgcgt ttcctgcaga
     1681 tccgcaagtt tccctacgcc atgaaggtga cactgggacg tatgatgaag aaaaaggaag
     1741 cctccgatgg agccctgaca ccttttcaag cggtatgtac tgctctcgcc gctacggtag
     1801 gcacgggtaa cgtagccggt gttgcaggtg ctatcgctat cggcggcccc ggtgcggtat
     1861 tctggatgtg gatttccgca ttgcttggta tgtgcactaa gttcgccgag gtaacgctgg
     1921 ctgtacactt ccgtgagacc aatgcacagg gcgacctggt aggcggccct atgtattata
     1981 ttaagaacgg actggataag aaatggcact tcctggcgta tttgttcgcc gctttcggtg
     2041 tgctggctgt gttcggtacc ggtaatgcca cacaggtcaa cactatcact actgccattg
     2101 attctgctct gagcagcttt aacgtactgc ctgcagatgc tgtgaagctt gtcaacctaa
     2161 tcatcggcgt ggcactggcg atcatcatcg ccctgatcct gatcggcggc atcaagcgta
     2221 tcggcaatgt cacctccaag ctggtacctt tcatggcgat catgtatatt gtgctggcac
     2281 tgggtgtgat cattttccat attaagtctg tacctgctgt attggcttct attatcgaag
     2341 gtgctttcca tcccgcctct gtgaccggcg gtgtggtagg aagcttcttc atgagcatga
     2401 aaaaaggcgt atcccgcggt atcttctcca acgaagccgg tcttggtacc ggatccatcg
     2461 cccacgcctg tgccgatacg aagaagccgg taaaacaggg cttcttcggc atctttgagg
     2521 tattcgtgga cacgatcatc atctgtacca tgacagcgct ggtcatcctg tgcagcggcg
     2581 tgcccgtaaa ttacggtgaa gctgccggtg cggagctgac catcagcgga ttcaccgctg
     2641 tctacggcaa ctgggtatct atctttaccg ctgtggcgat gtgctgcttc gctttctcca
     2701 ccatcattgg ctggggactg tatggtacca gatgtatcga attcctcttc ggttcccgct
     2761 ccaacatgcc tttcatggtt ctgtattccc ttacagctat cgtgggtgct acaatgaacc
     2821 tcggactgat gtggagcatc gccgagacct tcaacggact catggtcatc cccaacctga
     2881 tcgctgtctt cctgctgtcc ggtgttgtag tgaaactgac gaaggaatat tttgcgaaag
     2941 agaacgggta atatctgaat gggatttgcg ttatgatcat tgaataaatg atgtagttct
     3001 ttcgttcgat aatgacagtg aattgaaagt tagtgaaata
//
LOCUS       DAWEJW010000186         2993 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8525_length_2993_cov_60.849217, whole genome shotgun sequence.
ACCESSION   DAWEJW010000186 DAWEJW010000000
VERSION     DAWEJW010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8525_length_2993_cov_60.849217"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          738 a          770 c          531 g          954 t
ORIGIN      
        1 ttatctaatt aactacatta ttttctgtct aaaatatcca taaattcgtc acctgtaatt
       61 gtttccttct catacaggta catagccagt tcgtccagtt tttccctgtt ttcagcaagg
      121 atttttcttg ctttttcatg ctctgctttt acgagctgca cgactttttc atcaatttcc
      181 ttctgggtat ctgcggaaca ggaaagagac gtatcgccgc caagatactg gttggtcacg
      241 ttttccattg ccaccatgtc gaattcatct gtcatgccgt agcgggtgat catcgctctt
      301 gcaatttttg ttgcctgttc gatatcattg gaggctccgg tcgtgatctc accaaatacg
      361 atctcctctg ccgcacgtcc gcctgtaaat gtagcaatct tattctcaag ttcttttttc
      421 gtcatcagat atttatcacc ctgctcaacc tgcatagtgt agccgagtgc acctgaggta
      481 cgcgggatga tcgtaatctt ctggaccggt gctgaatgac tctgtaatgc agctaccaga
      541 gcgtgtccta tctcatgata tgcaacaacc ttcttctctt gatctgaaag aaccgcattc
      601 ttcttctgat atcctgcaat aaccacttct atactttctt cgagatcaga ttcatttaca
      661 acggtccttc cactacggac agcacgtaat gctgcttcat ttataatatt ggcaagctcc
      721 gcaccggatg cacccgatgc catacgtgca atcgtatgca ggtcaacatc atcagatgct
      781 tttatcttct ttgcatgaac ctttaaaatt gcttcacggc ctgcaagatc cggcagttca
      841 accggcacac gcctgtcaaa acgtcccgga cgtgtaagtg ccggatctaa cgactccgga
      901 cggttcgttg cagcaagaat gatgacacca ttatttcctt caaatccgtc catctctgta
      961 agaagctggt ttaaggtctg ctccctctca tcatttccgc ccatttgtcc gtcacgcttt
     1021 ttaccaatgg catcaatttc atcaataaac acgatacatg gtgccttttc ctttgcctgt
     1081 ctgaaaagat ctcgaacctt ggaagctccc ataccgacaa acatctcaac gaattctgat
     1141 ccggacattg agaaaaatgg tacatttgat tcacccgcaa ctgcttttgc aagcattgtt
     1201 ttgccggttc ccggaggtcc tacaagaagg acaccttttg gcatagacgc acctacatcg
     1261 gtatacttct tcggattgtg aagataatca acgatctctg aaaggttttc tttcgcctca
     1321 tcttctcctg caacgtctga aaaacggatt ccctcactgg attgcacata aatcttcgca
     1381 ttgctttttc ccattccaaa agccatcgaa ttttttcctc cggcatgctc catcagtttc
     1441 ttcgccatat aatttcccag tgcgataaaa aggatcaatg gcaaaacccc ggtcagcagg
     1501 aagctgatga tcggtgacat ctgcttatcg atatcttttg cgaatactgc tccacattca
     1561 tatagcctgt ccgtcaggtt cggatcgttc atcagacctg ttttataaat atttttttga
     1621 tctttatctg taaagatgat ctgattgtcc tcaacttcta cttcaccaat attcttcttc
     1681 tcgatcatgc tcataaaagt gccataatcc gtttccttca cctgatgctc cataaggatt
     1741 ggtgtgacaa ccagattaaa tacgatcagc actattaaca caatcccata ataatatgcc
     1801 agtggttttt tcggtgtttt aacttctttc atggtctttc ctcctgttgt tctgcgttat
     1861 gctttctccg tgcattttcg tattatttat tctgccatct gacttcgccc ttggacagaa
     1921 gcggataaca gatcagctga tctgaatcca gattttccgc atgcatgtca accgctgtga
     1981 tctgatgatt atcataagtc gtataataat aaataccttt tgctgtatta caacaggacg
     2041 tgtatatcgt gatctcatat ttgccttctg tcacctcaca gcatccacgc tgctgatcta
     2101 cggatcctaa gatatggaag aactggctta cactctcatc ctcttcttca ccggagatcg
     2161 aatttaattt tgtaaaggca actttcacaa atcttgactg gctggaaaga tctccaggta
     2221 ttcccattcc acccattcct ctgctatatg catcaagctg taatactcct gcaaaagtac
     2281 tctccggctg ttttctggat actccggcat aattattcag tgcaaacatc tgactcggga
     2341 atggtggatt attggtaagc actcctaccg gattatcata tacatgcagc ccatctttca
     2401 tagactcaac aacgatacag gagtctttgt ctgcaatgat ccagtgaagc tgtgctgtcg
     2461 gcagctgttc actgaatgct gtgcctgtca gtctcattgc tgccagcttc tctctcgcct
     2521 ctgctacagt agcacactgt gtaaggatcc atgggatgaa ctcgaactgt gccacctggc
     2581 tgacttctgt ttcatcttcc ggcaaagcct cttcatatgc cgcattgccg acaaaattaa
     2641 gacctgccat tccaaggcct ttttcattaa cagcatcata ataaagcgga taaccttctg
     2701 caacaaatgc cattccgatc aaagcataat ggctttttat cttcccggca aaccggaaat
     2761 caaattcgta atttcttggc gttatcgtga tctgttcccc ataggaaaat tcataatcaa
     2821 gcgttctgcc catataaaaa tcttttgttt tgtaagttgc tgctgtacac atatcgaaca
     2881 cctcttcttt ctgctataat agtcctgcaa acaatctcat aaacaactgt ccgagccgta
     2941 aatatgcact ccgtcctgtg cggtactttt cggtcacttc cctgcattca ttc
//
LOCUS       DAWEJW010000187         2983 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8559_length_2983_cov_60.789617, whole genome shotgun sequence.
ACCESSION   DAWEJW010000187 DAWEJW010000000
VERSION     DAWEJW010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8559_length_2983_cov_60.789617"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          832 a          613 c          829 g          709 t
ORIGIN      
        1 ggatctgcaa ggggatcatg ggcggaagga tgctggggga aatcctgcct ttttacatag
       61 taagtttttt agggatgtat ctgtattttt ctctgtcggc catgatggat atcaagggga
      121 aacgcagggt tctgaagacg aatctcatag attatctgga aaatcatctg tcgggccgga
      181 tctctgtcac ggaacaggat tatgaaagac tctacggacc gggaagcctg ggggaggaga
      241 tcagtgcggc cggaagaacg agaggacgac agcagagagc ttccggcagg cggacggtgg
      301 aactgatgcc gatcagcagc agaatggcgc agccggaaga aacgggagat cgtctggaca
      361 gagaagtatt ttccgtagaa aatgccgcac atgaaaagcc gggagaaaaa gtgctgccgc
      421 agagaccgga ggatcccgcc agagtcacgg aggaagaatt acaggcattg cttcaggaat
      481 tacttacagt atagcggaaa atgtcagacg tccgaaagaa ttcagaaaac attccccgga
      541 tctatagata tgtcttgaaa atgagatgta actttgttaa aataaaaacg gattttttaa
      601 gtaagtaacg caagaatgcg tgatatatct gcaagatata aggaggagca ttatgagcag
      661 acaagttaca tttgattatt ccaaggccgg ctcttttatt tctgaagaag agatcggtta
      721 catgaagaag cttaccctgg atgcgaagga gaccctggtg tccaagaccg gtgcaggcaa
      781 tgattttctg ggctggatcg accttcccgt tgattacgac aaggaagagt ttgcccgcat
      841 caaggcagca gcaaagaaga tccagagcga ttccgacgta ctgttagtca tcggtatcgg
      901 cggttcctat ctgggagcca gagcagctat cgagttctta agccacagct tctacaatgt
      961 actggataag agtgtacgta agactccgga gatctacttc tgtggtaact ccatcagctc
     1021 cacctatctg aagcacctga tggatgtagt aggcgacaga gatttctcca tcaacatgat
     1081 ctccaagtcc ggtaccacca cagagcccgc tattgctttc cgtgtattca aggagaagct
     1141 ggaagctaaa tacggcaaga agggcgcggc agagagaatt tatgctacca ccgataaggc
     1201 gaagggaagc ttaaagcatc tgtccgatga ggaaggttac gagaccttcg tagtacccga
     1261 tgatgtagga ggacgtttct ccgtactgac cgctgtaggt ctgttaccga tcgctgtcag
     1321 cggtgcggat atcgacaagc tgatggaagg tgcagccagc ggaagaaagc gtgccctgga
     1381 gaatgctttt gaagagaacg atgctttaca gtatgcggct ctgcgtaaca ttttactgcg
     1441 caagggtaag agtgttgaga tcctggcgaa ttatgagccc gctgttcact atgtaagtga
     1501 gtggtggaag cagctgttcg gtgagagcga aggtaaggac aacaagggtc tgttccccgc
     1561 aagcgtggat ctgaccaccg atctgcattc catgggtcag ttcattcagg acggttcccg
     1621 tgtaatgttc gagaccgtga tcaatatcga cactccgaga gaggagctta ccatcggcga
     1681 ggagcctgta gatctggatg gtctgaacta tctggcaggc aagaccgttg atttcgtcaa
     1741 taagagcgct atgaacggta ctattctggc acataccgat ggccaggtac ctaacttcat
     1801 ggtgaccgtt cctgaagtaa atgagttcta ccttggtgaa ctgttctatt tctttgagtt
     1861 cgcatgtggt gtcagcggct atctgctggg tgtaaatcct ttcaaccagc ccggtgtaga
     1921 gagctacaag aagaacatgt tcgctctgct tggcaagccc ggttatgagg cacagagaga
     1981 agaactgctg aagagattgt aagccacaga ggcgggatca ctccggcagt aatatgtttc
     2041 aaaagaagaa aaggacagcg taatgaattg atttcattac gctgttgctg tatgatggaa
     2101 taagtaagta gggcgatagc ccggaaaaat cgtttattct actatgttat tatgctatta
     2161 cgaaaagaaa acaaaagaca ggagaaattg caagagatga agattgtttt actggagaga
     2221 aacagcgccg gcacggatgt gccggtggac tgctttaatg aattgggaga agtgaccagt
     2281 tacccgaata ccgttactgc acaggaagtg gcagagcgtg tgggcgatgc ggatgtcatt
     2341 gtctgcaaca aggctcccat gaaagaggaa tccctgaagg attgccccaa tgtgaagctg
     2401 atctgtgagc ttgctaccgg atacgacaac tgtgatctgg catattgcag gtccagagga
     2461 atcaaggtgg caaatgtggt cgattattcc accgccatgg tagcccagca tacctttacg
     2521 ctggcactgg ctttgagcca gaaactgccg tattatgaca gatatgtcaa gtccggtgcc
     2581 tacagcgcgc aggatcgttt ttccaatttt gcccagcctt tttatgaact ggaaggcaag
     2641 acctggggta tcgtaggaat gggcaatatt ggtaagagag ccgcagcaat cgcaacagct
     2701 ttcggttgta aagtaatttt ctattctatc actggtaaga gtacctgtac cgaatatccg
     2761 caggtggaca aggataccct gttaaaagag agtgatttcc tgtccctgca ttgtccgttg
     2821 agtgatctgt cccgcaactt cattgataaa gaagcacttc gcaagatgaa gaagaccgca
     2881 attctcatca atgtggcacg tggtcccgtg gtaaataatg cagatctgta tgaagcactg
     2941 gtggccggag agattcaggc agcaggtctg gatgtcctgg aaa
//
LOCUS       DAWEJW010000188         2980 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8567_length_2980_cov_52.422222, whole genome shotgun sequence.
ACCESSION   DAWEJW010000188 DAWEJW010000000
VERSION     DAWEJW010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8567_length_2980_cov_52.422222"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          699 a          825 c          619 g          837 t
ORIGIN      
        1 tttccaggac atccagacct gctgcctgaa tctctccggc caccagtgct tcatagagat
       61 cagcgttatt taccacgggg cctcttgcca cattgatgag gattgctgtc tttttcatct
      121 tacgaagggc ttccttatca atgaaattac gggataagtc acttaacgga caatgcaggg
      181 acaggaaatc gctctctttt aacagggtat ccttgtccac ctgcggataa tccgtacagg
      241 tgctcttgcc cgtgatggaa taaaagatta ccttacagcc gaaagcggta gcgatcgctg
      301 cggctctcct gccgatattc cccattccta caatgcccca tgtcttacct tctaattcat
      361 aaaaaggtct gtcaaaatta gaaaaacgat cctgtgcact gtaagcgccg gattttacat
      421 attcatcata gtagggcagc ttctggctca atgccagcgc cagagtaaaa gtatgctgtg
      481 ccaccatggc tgtggaatag tccaccacat ttgccacctt gatgcctctg gacttgcaat
      541 atgccagatc acagttgtcg tatccggtgg caagctcaca gatcagcttc acattggggc
      601 aatccttcag ggattcctct ttcatgggag ccttgttgca gacaatgaca tccgcatcgc
      661 ccacacgctc tgccacttcc tgtgcagtaa cggtattcgg gtaactggtc acttctccca
      721 attcattaaa gcagtccacc ggcacatccg tgccggcgct gtttctctcc agtaaaacaa
      781 tcttcatctc ttgcaatttc tcctgtcttt tgttttcttt tcgtaatagc ataataacat
      841 agtagaataa acgatttttc cgggctatcg ccctacttac ttattccatc atacagcaac
      901 agcgtaatga aatcaattca ttacgctgtc cttttcttct tttgaaacat attactgccg
      961 gagtgatccc gcctctgtgg cttacaatct cttcagcagt tcttctctct gtgcctcata
     1021 accgggcttg ccaagcagag cgaacatgtt cttcttgtag ctctctacac cgggctggtt
     1081 gaaaggattt acacccagca ggtatccgct gacaccacat gcgaactcga agaagtagaa
     1141 cagctcgcca agatagaact catttacctc gggaacggtt accatgaagt tgggaacctg
     1201 accgtcggta tgtgccagaa tggttccgtt catagcgctc ttattgacga aatcaacggt
     1261 cttgcctgcc agataattca gaccatccag atctacaggc tcctcgccga tcgtaagctc
     1321 ctctctcgga gtgtcgatat tgataacggt ctcaaacatt acacgggaac cgtcctgaat
     1381 aaactgaccc atggaatgca gatcggtggt caggtccacg cttgcgggga acagaccctt
     1441 gttgtcctta ccttcgctct caccgaacag ctgcttccac cactcactta catagtgaac
     1501 agcgggctca taattcgcca ggatctcaac actcttaccc ttgcgcagta aaatgttacg
     1561 cagagccgca tactgtaaag catcgttctc ttcaaaagca ttctccaggg cacgctttct
     1621 tccgctggct gcaccttcca tcagcttgtc gatatccgca ccgctgacag cgatcggtaa
     1681 cagacctaca gcggtcagta cggagaaacg tcctcctaca tcatcgggta ctacgaaggt
     1741 ctcgtaacct tcctcatcgg acagatgctt taagcttccc ttcgccttat cggtggtagc
     1801 ataaattctc tctgccgcgc ccttcttgcc gtatttagct tccagcttct ccttgaatac
     1861 acggaaagca atagcgggct ctgtggtggt accggacttg gagatcatgt tgatggagaa
     1921 atctctgtcg cctactacat ccatcaggtg cttcagatag gtggagctga tggagttacc
     1981 acagaagtag atctccggag tcttacgtac actcttatcc agtacattgt agaagctgtg
     2041 gcttaagaac tcgatagctg ctctggctcc cagataggaa ccgccgatac cgatgactaa
     2101 cagtacgtcg gaatcgctct ggatcttctt tgctgctgcc ttgatgcggg caaactcttc
     2161 cttgtcgtaa tcaacgggaa ggtcgatcca gcccagaaaa tcattgcctg caccggtctt
     2221 ggacaccagg gtctccttcg catccagggt aagcttcttc atgtaaccga tctcttcttc
     2281 agaaataaaa gagccggcct tggaataatc aaatgtaact tgtctgctca taatgctcct
     2341 ccttatatct tgcagatata tcacgcattc ttgcgttact tacttaaaaa atccgttttt
     2401 attttaacaa agttacatct cattttcaag acatatctat agatccgggg aatgttttct
     2461 gaattctttc ggacgtctgg cattttctgc tatactgtaa gtaattcctg aagcaatgcc
     2521 tgtaattctt cctctgtgac tctggcggga tcctccggcc gttctgttat cgccttgcct
     2581 tccgcctttt cgggcgttgc attttccgtg gaaaatactt ctctgtccag acgatctgcc
     2641 gtctcctccg gctgcaccgc tctgctgcgg atcggcatca gttccaccgt ccgtctgccg
     2701 gaagtacgct gccttcttcc cgcctttccg ccggttgcga tctcctccac cagacttccc
     2761 gggccgtaga gtctctcata atcctgctcc gtaacgggga tccggcccga cagatgattt
     2821 tccagataat ctatgaggtt cgtctttaat attctgcgtt ttcccttgat atccaccatg
     2881 gctgacaagg aaaaataaag atacattcct aagaagctta ctatataaaa aggcaggatt
     2941 tcccccagca tccttccgcc catgatcccc ttgcagatcc
//
LOCUS       DAWEJW010000189         2944 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8654_length_2944_cov_74.687089, whole genome shotgun sequence.
ACCESSION   DAWEJW010000189 DAWEJW010000000
VERSION     DAWEJW010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8654_length_2944_cov_74.687089"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          741 a          717 c          707 g          779 t
ORIGIN      
        1 attatataag actctcaacg agaaatcaat ttgtggatga tttgactttt tgcgtcacaa
       61 atgtcagcat gtctcgcctg agggattcag tagcttctac gcctttcgcc ggcgtctggt
      121 atcactggga ctgacaccat aagcattgcg gtaggttctg gaaaaatgca ccaatgacgc
      181 aaatccggtc ttctgcgcga tctcctggat cgccagagaa gactccacca gaagtcttcc
      241 ggctctggtc agccggacat tggtaaggta ttccagaacg gtggctccgg tcacctcgcg
      301 gaaaatacgg caaaaatagt atttacttaa atggatctgg gatgcgatgg tctccagatc
      361 tattttttcc gcataatggg cattgatata ggcgatggca tccagaatct cctgtctggg
      421 agctgcggag agctccggca tctgtgtccg ctcctgcctc gtatagtagt ctctgcacag
      481 tagcagcagt tccagacaat agttttgctt taatttctcg gccacagcat cgtggcggtt
      541 ataatatttc cgcagccgtt cgaagacctc taccagttct ccggactgtt ccgttgtcag
      601 atgcagaata tcattctgca gaacctccag gaaccgctgc ttctccacag gtttcaacat
      661 tccatccaga taatccggtg caaaattcaa gacataccgg gcatagaaat ccgaggacag
      721 actctgcata taatgcgtct ggtagggttt gatcaggatg atgtctcccg gattcagcac
      781 atgacagaca ccatccagga agatgtatcg tatccccttc accacaaaaa aaagttcgta
      841 agtatcgtga taatgattgg tctgcatatc attttttttg gttgtctcta tgtattccag
      901 aaacagatcg gaataagggt caaaaatcgt acgaagcata gttttccttt cccggaatca
      961 ttccggcaag attcattcga ttgcttttta ttaccgcaag atatgttaaa caatcttaaa
     1021 aataaaagat tataatggga ttatataata aatcttgtgc catgtgaagt atattggaag
     1081 gagataactt atggagaaaa gacctttaga ctatgcaaaa gcatccatgg ataccatgat
     1141 gcgcaaatgg gaagccccca agcttccgcc cgaaggaaga ttccattatc atcagggagt
     1201 attcctgtcc ggaatgtaca aaagctatga actttgcaaa gaggaaaaat attttgatta
     1261 catcaaatcc tgggtggatg ccgtcatcac cgaagacggt gtgatcaaac aggctgatat
     1321 ggggcagttc gacgatatgc agccgggtat tctgttatat cccctctatc attgtaccgg
     1381 tgatgccaga tataagaaag ccctggactc tctgatggct gccattgatc gttatcccac
     1441 cactcccgag ggcggctatt tccacaaggc tgatctgccg gaagaaatgt ggctggacgg
     1501 tctgtacatg gaaggtccgc tgcgcgcgca gtatggcgca gaattccatc accccgaata
     1561 ttttgatgag gttatcttcc aggctctgac catgcagaaa catacccgtg accccaagac
     1621 cggtctgctg tatcatgcct ggtcctatga caggaaagta gaatgggctg ataaggagac
     1681 cggctgctcc cccgaattct ggggcagagc catgggatgg gtgcctgtag ccctcctgga
     1741 cgaactggat ttcattcccg ccgatcatcc ggacagagca aaactggaaa aaatggcggt
     1801 agatctgtta aaatctctgc ttgcattcca gtctgaagac ggcagatggt atcaggtagt
     1861 caaccggggc gatgatcctg ccaactggct ggagaattcc tgcagctgcc tgtatgttgc
     1921 cgccctgtgc aaagcgatcc gcaaaggtct gctggatgca tcttatctgg aatatgcccg
     1981 caagggttat gagggtgtga tccgctccct gacctgggaa ggtgatgacc tcattatcgg
     2041 taatgtctgc atcggaaccg gtgtcggtga ttatgaccac tatatccacc gtcccgtatc
     2101 cgccaacgac ctgcacgggg tcggcgcctt cctgctgatg tgtgaagagt gcgaacaggt
     2161 attttaatat cttgtaacta ttcagagcca tgcaaaacca ggcttacaga tcctcgctgc
     2221 cgggattccg gcggcgggca gatctgctta tattattact ttttacagct ttacttttac
     2281 aaaatcatgt tccatggcgg gaagcacctt ttcgatcaga tccgccgtcc gcatgcggag
     2341 gctggaggta ttgatgcagg ggtggcatcc gaaatattcg ctgtccagca cctcttcatc
     2401 gatcaacagc ttcacctgat gttccttatc gttcatcaga ccgagtacac tgacagagcc
     2461 gggggtgata tccaggaatt tctccatgta ttccggcttg gcaaaagaaa gcctcgccga
     2521 gccgatctgt gccgacagat ccttggtatg gaacaccttg gtatccggga tcatcagcag
     2581 ataaaaggcc gtctcctgac ggttgcacag aaacagattc ttacagacca tggactccag
     2641 gatctcatcc acttccttac agtcctccat ggtcatcgcc ggagcgtgat ccacacggtc
     2701 atattccacc ccgagcctgt ccagcaggtc ataggtacgg atctctttgt ccagtctgcc
     2761 ggtcgtatct gcaggtcttc ctttttgtaa ttccaatgcc atgttctttc ccctttgctg
     2821 tttaatatcc gatggtgtca tttacaagaa tcacatgaat tctgtccgta tgtctggaat
     2881 atcttgtgat caggaactga cagcagatgg tatccggaga tttgcagtcg aagcattttc
     2941 cggt
//
LOCUS       DAWEJW010000190         2911 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8756_length_2911_cov_53.868697, whole genome shotgun sequence.
ACCESSION   DAWEJW010000190 DAWEJW010000000
VERSION     DAWEJW010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8756_length_2911_cov_53.868697"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          621 a          853 c          608 g          829 t
ORIGIN      
        1 gtgactttct tgcgtccatc ggagcgcagg gaaatatttg ttaccatggt attcaacaaa
       61 taagggattt ttgcttcttc tgccatgtca atatagcgtt ccgcatactc cggtccggtc
      121 agctcctctt taaaagtatg cagtccgaaa ccgttgtgaa tacactggtt cagaatgcct
      181 cccagttctc tgtcacgctc taagatcagt actttttcac agccggcctt tctcgcagac
      241 agcgctgctg ccagtcccgc gggaccgcct ccgatgatca caatatcttc cgtacgttta
      301 ctcatggagt tcctcctttc cgaccggtgc ctgtggcttt ttccacgctc cgccctctcc
      361 gggtgcggtg tctttatttt taccggtcag aatctctgag ccggttcctt ccttagtgat
      421 ctgtgccgga tccagctgca gttctctcgc caggatctcc agggtcttgg gagagcagaa
      481 gcccgcctga catctgccca taccggctct ggtacgacgt ttgacaccat ccagtgtcgt
      541 tgctcctact gggcggtgaa tcgcttccag aatctctcct tccgtcacca gctcgcagcg
      601 gcagatcaca ttggcaaacg caggattttc ccggatcagt gcttctctct cctcttccgt
      661 ggcaagtgcc atgctgggaa tgccttttct gctgtcaata aaatccgtct tacgttctgc
      721 gggataaata ttttcaacaa tccgtgccac atactcaccg attgccggtg cactggtcag
      781 tcccggggat tcaatacccg ccacatcgat gaacccggga tgccctgctg cctcttcaat
      841 aaggaaatcc gatttttccg caggagcttc gtgtaccaga tgagctcgca gtccggcaaa
      901 agaggtgatg gtctgtctgc cgtccagcgc gtccacactg ttacttccca gattcagaac
      961 cttcgtcagt ccttctgctg tcgtatctac atcttctttg tcaggaatat cttccgctgt
     1021 gggacctacc agtaaattgc cgtgtaccgt gggagtaacg aggatccctt ttcccatttt
     1081 ggacgggagt tggaaaacgg tggaattcac cagattaccg caatttttgt ccataagacg
     1141 gtactcacct cttctgggga caatacggat cctgtcttca cagatcatgt tatggatctc
     1201 atccgcatag acacctgccg cattgataac gatgggagca aggaacatct ctccatccgc
     1261 ctgtacctga tacatccggc cttctttctc gggtggattc tcactccata ccggtctctg
     1321 ttccacaatg gatgtcacct tatggttccg atagaactcc acaccattct ccgctgcatt
     1381 ctccgccagt gcaatgttca gtccgaaggg acagacgata ccaccggtgg gtgcatacag
     1441 ggctcctacg atgtcacggg acaccgccgg ctccattccc cagacttcct ccggggacaa
     1501 aagcttcaat tcttttaccc cgttctccat gcctcgccga tacagttctt ccagaccgga
     1561 catggcatct tcttcaaagc ataacaccag ggatccgttc ctgcggaacg ggaagtccag
     1621 cttcttcgac agttcctcca tcatttcatt gccccggacg tttaactgtg ccttgagagt
     1681 ccccggtact gcatcatacc ccgcatgcac gataccgctg ttggctttgg aagtaccttc
     1741 gcagacatca cttttcgcct ccagtacagc gatccttagc tttcccttcg ccagttccct
     1801 ggcaatggcg cagccactga ctccggcgcc gataatgatc acatcataat ccttcataac
     1861 ctcgccctgc ctttcctatg tttacattta tatttgaatg tccaccttta ccttctgaat
     1921 atccgttttc tctttatgaa tgtccatttt ttatttgtcg caaatgtctc tttgctattt
     1981 attttaccat attctgcggg aaaacacgac cgaaaaccag caacactctg tgtaaaatgt
     2041 agtcgaaaaa accggacagc aatcgctgtc cggtcagttt tctcttataa caatgtattc
     2101 agatccacca gggttccgcc ctcgatctcc acgcacttgt cgctgtttaa ctgcagccat
     2161 acgctgcagg cattggggtt atataccttt tcgtaatccg cagagaactg taaacgtttt
     2221 gctgcatcca tgtaatcaat gatctcgatg ttggtggcgt accagatgtc gtctctgccg
     2281 cccatgtatt tacagaagtt ctcgatcaca tcccagttgt cattattatc gaactcgtag
     2341 ctgtgccccc agacgtacat caacttcagg tactggctct tcttgaaatc agcaaaaaat
     2401 tccgcgtact ccatcagctt cggatcatta tgatggcagg tagcatgcca ctccaacgga
     2461 tcctttggca gtgcaaaatc cggaaccgtc tctaccactc tggcataagc cacgcccagc
     2521 tggcgataca gctgtttgat ctcctcgctg taagaaccgt tgggatacgc atgtcccctg
     2581 acgatctgtc ctgtccattt ctccagacct ttccgatcct ccagaagctc ctgtgccact
     2641 tccactaacg gacatctctc gatggtagga tgcgtcatgg tatgggttgc gatctcatgt
     2701 cccgcataca gcccttccat ttcttcaaaa gtcatacggg ggcgttctcc cacctgaccg
     2761 taattcagat taaacgttcc cttgatacca tatttattga aaatgtcgat caggcgtctg
     2821 tcctgtacct taccgtcatc gtaactcatg gtcaatgctt tggcttttcc gccgggaaaa
     2881 caagtatata ctttcatctt ttccatcctt t
//
LOCUS       DAWEJW010000191         2887 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8849_length_2887_cov_53.170551, whole genome shotgun sequence.
ACCESSION   DAWEJW010000191 DAWEJW010000000
VERSION     DAWEJW010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8849_length_2887_cov_53.170551"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          774 a          587 c          865 g          661 t
ORIGIN      
        1 tctgagacac ttgagagtgc agagagcggc aagtaataca tatgatgcca gggtcctatg
       61 tgccagtggc acatgtttag cacggaccga agcggagcgg agacgtggga gtgcgtagca
      121 cggagcaatc cgcaggcaca aatattgcag gctatgaaag gcatgaaaac tttgaggtca
      181 ggaagaaata gaagaaaatg gatctatgtt ctgcttctta tgggactgct ggggctaagt
      241 ggctgcggca gtccttcttc tgcttctatg gagacatcat cggaagcagc atctgaggtt
      301 acccagacag cagagaccac gcaggaagaa cagacaaaat tagtaaagac aggcagcatg
      361 gagctgcaat atgcggaaaa ttttacagtg gactattacg aagatggtta taagatgctg
      421 acaactcttc ccgacagtca gcggtatctg ttagtgccgg agggacagcc ggtaccacag
      481 ggactggatg aggacgtcag tatcttgcag gaaccacttc aaaatatata tctggtggca
      541 tccggcgcga tggacatgtt cgcctcgctg gacgctgtgg atcagatccg gttctccgga
      601 cagaagcagg agaactggta cattcagaca gcaaaggatg ccatggcggc aggggatatg
      661 atctatgccg gaaaatacag taaaccggat tatgaattac tggtgtctga aggctgtgat
      721 ctggcgatcg agaaccggat gatcacgcat tcgccggagg tggtggaaat gcttcaaagc
      781 tttgacattc cggtgatgat cgaatattcc agctatgaac agcatccttt gggaaaagtg
      841 gaatgggtca aatttttcgg agctctgacc ggaaaggaag cgaaggcgga agaagccttt
      901 gcggagcaga cagaaattct ggaagaggta gaaagcgggg aaaaaacagg gaagaccata
      961 gcgtttttct atgtgacttc caacggactg atccaggtac ggcagagcac ggattatatt
     1021 ccgaagctga tcgagctggc agggggaaga tatatttttg agaatctgga agacagcgga
     1081 agcggacgct ccacgctgaa catgcaggtc gaagattttt atgccggagc gaaagacgca
     1141 gacatcctga tctacaacag ttccattgac ggaggcgtga ccacggtgga tgagctgatc
     1201 ggcaaatgca atattctaaa agacttcaag gcggtgcagg aggggcaggt ctattgcacc
     1261 accaatgata tgtaccagca gtccatggca atcgggtatt tattgcagga tatgcatacg
     1321 gtgttgacgg acgatgatac cgctaagctg cgttatctgt ttctcttaca gtaagaagga
     1381 ggacttagaa acacagcgta agtataagag cgttagaatg agattagaat acgattggaa
     1441 cacgaatagg aaaagaagga gaaaaacatg cagcagcgca ggaaaatcag atatctcata
     1501 gcgtttgttc tattgatagc aggcattttt gtgctgttgt tttgcaatat aggaatcgga
     1561 acggtgaaaa ttccactggc agaaatttta accacaggga gagcacaggt aggcatgaac
     1621 gcaaagatcc tgtgggagat ccgttttccc cggacactgg cggcggggat cctgggcggt
     1681 gcgctggcac ttgccgggta tctgttgcag acctttttcc acaatcccat agccggacct
     1741 ttcgtcttag gaatttcttc cggagcgaag atggtagtgg cactggtgat ggtttttttg
     1801 ctggggaata cggtccgggt cagctcagga ctgctgatcc tggcggcatt tctgggtgct
     1861 atgctgtcca tgggatttgt gcttcttatg gcgcgaaagg tggacagtat gtccatgttg
     1921 gtggtcagcg gtgtgatgat cggctacatc tgttccgcca tcacggaact ggtggtgacg
     1981 tttgcggagg acgcggacat cgtgaacctc cacaactggt ccagaggaag tttttccgga
     2041 atgacctggg ataatgtgaa agtgatgagc gtcgtggtgg cggcggcttt tttgttggtg
     2101 attcttttgg caaagccgct ggaagcctac cagctgggag aaacctatgc gcagaatctt
     2161 ggagtgaata tccggaccct gcggatcctt ctggtggtgt tgtccagcgt gctttcggca
     2221 tgtatcgtgg catttgccgg accgatctct tttgtgggga tcgctgtgcc gcagctgatc
     2281 cgtaaactgt tcggcactac aaagccgtta ctgatgatac cggcatgttt cttaggaggc
     2341 agtgtattct gcctgttctg tgatctgctg gcgaggaaat ggatggcacc cacggaactt
     2401 agcatcagca cagtgacggc catcttcgga gcaccggtag tattgtggat catgatatca
     2461 cgaaacagac aggagcgtgg ataactatgg aaacgcagaa gtcttattat attacaacgg
     2521 aaaaaatgag cgtgggctac gacggaaagc ctctgatcga agaagtggag attatgcttc
     2581 gaaaagggga aatcctgacg ttactgggtc caaacggtgc aggcaaatcc acgatcttaa
     2641 aaagtattgc aaggcagctg tcactgatcg caggaacggt gcggctggac ggagaagata
     2701 tgcggacact caccggggca gaactgtcga aaaaaatggc agtggtaatg acaaaacgtc
     2761 tgcgggggga actgatgacc tgtgaagatg tggtggccac cggaagatac ccttataccg
     2821 gacgctttgg cgtgctgcgg gcagaagatc atgcagcggt gcaaaacgcc atggaactgg
     2881 tgcaggt
//
LOCUS       DAWEJW010000192         2860 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8942_length_2860_cov_66.845633, whole genome shotgun sequence.
ACCESSION   DAWEJW010000192 DAWEJW010000000
VERSION     DAWEJW010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8942_length_2860_cov_66.845633"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          804 a          661 c          700 g          695 t
ORIGIN      
        1 aatttaaaat gtaaaaaata tttaagctct atttttattt tcccctacat catgccccag
       61 tggacactgg ctgtggtatg gcagaatgtg ttcaacagca atgcagtcac cggaacctcc
      121 aacggactgc tggcatctct ttttaatatt acgatgccaa aatggtggtg cttgggattg
      181 ttccccagtg cagtggtatt gggactgcat tacgccccct ttgcctacat cctgatcggc
      241 ggtatcttcc gcaacatgga tgccaatctg gaggaagcag cgacgatcct ggatacaccg
      301 aagtggaaga ccatgttccg cattacctta cctatggtaa aaccggcgat tctttccacg
      361 atcctgctgg tattcggaag cgccatggga agttatcccg taccgcacta cctgggactg
      421 accactctgt ccaccaagta tatctccatg aactccaagt ataccgggga agccagtatt
      481 cttgccatca tcatgatgat cttcggtgtg gcgatcctgt taatgaacca gatgagttta
      541 aagagccgta aaaattatac gacggttacc ggtaaatctg gacagatctc caagatcaac
      601 ttaggaaaag tcggaaaata tctgatcgcg gtcatcctga tcgtcatcac cttcttcacc
      661 agtattttcc cgatcctgtc cttcgcactg gagactttcc tgccgaatcc gggagattac
      721 agtttcctgt ataccatgga ttccagtgcg ctgaccacca agtggtggct gacggataac
      781 aacggagccg gtgctctgta cggacagccc ggtattcttc acaatccgct gatctggaga
      841 gctttcaagg gaacgatgtt agtcgccgtc tgctgtgctc tgatcgcagg taccatcggt
      901 accctgatcg gttatgcggt atccaagaac cggagaagta aatgggccaa ttacgtgaac
      961 ggtgtggcat tcctgccgta cctgatgcca tccctggcag tgggcgcagc cttcttcatc
     1021 ctgttctcca atgacaagat caacctgttc aacacttaca cattgttgat catcgcaggc
     1081 gtgatcaagt acattccttt cgccagcaga agtgcactga actccatgct gcagctgtcg
     1141 ggagagatcg aggaagcggc catcatccag gatattccct ggtacaagcg gatgctgcgg
     1201 atcatcattc cgatccagaa gtcttccatt atcaacggat atatgcttcc gttcatgacc
     1261 tgtctgcgag agctgtccct gttcttactg ttatgtacgc agggctttat cctctccacg
     1321 acactggatt atttcgatga gatgggactg tatgcattct ccagcgccat caacctgatc
     1381 ctgatcgtaa cgatcctgat ctttaatact ttagtaaata aactgaccgg gtccagcctg
     1441 gatgaaggta tcggaggtaa ataactatgc cggaaatcaa attaacacat gttacaaaac
     1501 gttggggaaa attctatgcg gtagatgatc tgagtctgga tatggaggat aattccttta
     1561 tcactctgct tggtccctcc ggttgcggta agaccactac cttacgtatg atcgccggtc
     1621 tggagacccc tatcagcggg cagatcaaga tcggagaccg ggtggtattc gatagtgaag
     1681 cggggatcaa tgttcccgca aataagagaa aagtcggatt tctgttccaa aactacgcac
     1741 tatggccgaa tatgacggta taccagaaca tctccttcgg actgggaaat atcaaggaag
     1801 aacttcccgt gatcgatgag gaagccaaag ctttaaaaag catgatcaag gcactggaga
     1861 atccgggaga gctggtaaaa ctcattgagg aatgcagaga taaaaaggga aagttagacc
     1921 tggatatggt ctatctgaag ctgatcgata attacaccat ttctatctat actgcaaagg
     1981 aactgtataa ttacaagctc catgaggcag cagacaaaga aagtgccgca aagcaaaaga
     2041 agcaggaact gactgcaaaa ttggacagca tcctggcagg tcataaggaa aaaggggaag
     2101 aacttaacga gaaattcgaa gtcgtatccg gtggcaaggt agtaatcagg gtcagaaaat
     2161 attccaagga agagattgac ctggcagtac gcagagtatc ccgtatcgtt aagatcggaa
     2221 tgttcatgaa tcgttatcct gcagaactgt ccggtggaca gcagcagaga gttgccattg
     2281 ccagaacact ggcgccggaa ccccaggtat tgttcatgga tgagccgctg tctaacctgg
     2341 acgcgaaact ccgtctggaa atgcgatatg aattacagcg tctgcatgta gagaccggaa
     2401 gtacgtttgt ctatgtgaca catgatcaga tggaagccat gacgctggca acgaagatct
     2461 gcctgatcaa taacggcgtt ttacagcagt atgagccgcc actgtccgtt tacagtagac
     2521 ccaataacct gtttgtagcg gatttcgtgg gaaatccttc catcaacttt atcgaggcga
     2581 agggagagca gaacgcacag ggcaatattg aggttaccat tctggatggc agaaaagcac
     2641 agtttattcc cggaaaacct ctggatctgc aaagatggtt cgcagagaga gataagaacg
     2701 aagccgatga ggcagcccgt cacaaagaga aaatgcagga taagaaagcc gtggagaaat
     2761 ccaacaagga tgaagtcttc aaatatcata ttgccagagt gaacgaagat gattatgcac
     2821 tgcaggaagc tcccgtgatc accaacgagg attttgtcat
//
LOCUS       DAWEJW010000193         2858 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_8950_length_2858_cov_48.376382, whole genome shotgun sequence.
ACCESSION   DAWEJW010000193 DAWEJW010000000
VERSION     DAWEJW010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8950_length_2858_cov_48.376382"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          777 a          653 c          709 g          719 t
ORIGIN      
        1 gcttacaccg gcacccgccc cgtggaatac gcggcggttc ccctgaccat gtacagcacc
       61 ggagcggatc atctggaatc ctacacctcc atgtccgccc tgttggagca tttttatgcg
      121 gagaaaaata ccctgaccag gatccgtcag aaatcttctg acctgcgccg tatcgtgcag
      181 accgctctgg aacgggagat taaaaaatac gatcttcagc tggcccagat gaaggatacg
      241 gagaagcggg aaaaataccg gatctacgga gaactgttga atacttacgg ttacagtgca
      301 aagcccggtg acaaatctct cacggcggta aattactata ccaacgaacc ggtgacgatc
      361 cccctggatc ctactctttc cgctacggaa aatgcgaaga aatattttga caaatacgga
      421 aagctaaagc gtacttatga agcattgtcc gaactgacta ctcaggtaaa agaagagatc
      481 gaccacttgg agaccatcag cacggccctg gatatcgccc tgaaagagga agatctggtg
      541 gagatcaagg aggaactgac ccagagcggc tacatccgca gaaagggcgg cacgaaaaaa
      601 gcaaagatca ccagcaagcc cttccattat ctgagcagcg acggatttca tatttatgtg
      661 ggaaaaaata atttccagaa cgatgaactg accttcaaat tcgccaacgg aaatgactgg
      721 tggttccatg ccaaaggtat tcccggctcc catgtagtgg taaagaccga agggaaggaa
      781 ctgccggacc ggacttttga agaggccggc cggcttgctg cctactattc taagggcagg
      841 gagcaggaaa aggtggagat cgactatgtc cagaaaaaac aggtcaaaaa gccggccggt
      901 gccaagcctg gcttcgtggt ctattatacg aactactctc tgatgatcga cagtgatatc
      961 tccggaattg aaaagatcag cgactgactt ttgacgattc cggcgcatct tatatggtcg
     1021 ttaccgtact atgcatcaga tagcatattt tatgcataaa tatactgtat atttttcatt
     1081 ctatgcattt atgtgcatat tttaatttgt tttattcata tttttgattt atttatgcaa
     1141 cttttctgct tgcttttgcg attataatgt tgtatagtaa ttgtcagagc gtaacacacg
     1201 atcctggcat ctgatactta cagattgctt cgtgctttgc acatcgcatt ttcagggctt
     1261 ttgcccctgg catcatacac actataaaac ggaggatttt atcatgaagg aaaaagtagt
     1321 tttggcttat tccggcggtc tggatactac cgctatcatt ccctggctga aggagaattt
     1381 tgactacgaa gttatctgtg tctgtgtcaa ctgcggacag gcagaggagc tggacggtct
     1441 ggaagagaga gcaaaggctt gcggcgcttc caagctgtat attgaagacg ttatcgatga
     1501 attctgtgat gattatattg ttccctgtgt acaggctcat gccgtatacg agaacaagta
     1561 tctgttaggt acttccatgg caagacccct gattgccaag aaactggtag agattgccag
     1621 aaaagaaggc gctgtagcca tctgccacgg tgcaaccggt aaaggtaacg accagatccg
     1681 tttcgagctg ggcatcaagg ctctggctcc cgatatcaag atcatcgctc cctggagaag
     1741 cgacaaatgg aacatggact cccgtgaatc cgagatcgaa tactgcaagg ctcatggcat
     1801 tcatcttcct ttctccaccg attcttccta cagccgtgac cgtaacatct ggcacatcag
     1861 ccacgaaggt ctggaactgg aagatcccgg caacgagcct aactacgagc atctgctggt
     1921 acttggtgtg actcccgaga aggctcccga tgagggtgaa tatgtgacca tgacctttga
     1981 aaagggtgtt cccacttccg ttaacggtga gaagatgaag gtctccgata tcatccgtac
     2041 cctgaacaag ctgggcggca agcacggtat cggtatcgta gatatcgtag aaaaccgtgt
     2101 cgttggcatg aagtcccgtg gtgtctatga gactcccggc ggtaccatcc tgatggaggc
     2161 tcaccagcag ctggaagagc tgatcctgga tcgcgatacc tatgcattcg agaaggaaat
     2221 gggtgcgaaa ttcgccagtc tggtatacga aggcaaatgg ttcactcctc ttcgtgaggc
     2281 agagcaggca ttcatcgaga aaacccagga atatgtgacc ggtgaagtaa agttcaagct
     2341 ctacaagggc aacatcatca aggccggaac cacttctcct tactctctgt acaatgagaa
     2401 cctggcttcc ttcaccaccg gtgatctgta cgaccatcac gatgcagaag gcttcatcaa
     2461 tctgttcggt ctgtctctga aggttcgtgc catgaagtta caggagaacg agaagaaact
     2521 tggtaactaa gaattaaaat aatgaggtgt ctatgataac tgcacagact ataattactg
     2581 tggtgatcct gtatctggtg atcatcaatg ttgtgggata tgtgatcatg ggtgtcgata
     2641 aaaaaagagc catccggggt gcctggcgca tctcggaggc ttctcttttt ttcacggcgc
     2701 ttttgggtgg cagtctggga tgtattctgg gaatgcagca cttccgtcat aagacgaagc
     2761 actggtattt caagtacgga atgccggcga tcctggtggt acaggtactg ctttttgccc
     2821 ttttattcaa ataggaaata tgctatactt cttttata
//
LOCUS       DAWEJW010000194         2828 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_9056_length_2828_cov_55.643347, whole genome shotgun sequence.
ACCESSION   DAWEJW010000194 DAWEJW010000000
VERSION     DAWEJW010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9056_length_2828_cov_55.643347"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          807 a          715 c          602 g          704 t
ORIGIN      
        1 tatcgttatt tccagctttg agaaatggta tgccaatcag gtcaaatatc ataaggtcaa
       61 tggcccgcca ccgggcgagg aactttgcaa acgatcctat tccgttcctg atgccgccga
      121 gatattgaag gtgaaacccg aaacgatcta tacactcatc cgacagggca agttgaaaac
      181 agaaacggca gacttctgta tgaggattcc caaagaggaa tttgagcggt ggtaccggtc
      241 acaaagtcgc taccgcacag cagccgaccg tgagcgtgac cgggagattg aagcgcaaac
      301 catctccatc ccggaaatgg caaaactact cggtatccca cgcaagaatg tttatgggat
      361 tcttgattgc aaaaaatacc gagattgttt cgtcattgag cgcgtggccg acaggccgcg
      421 cattaccaaa aaaagtttta aggcatggct taaaagccaa tctacatacc aacttcaaaa
      481 accgaaaaaa gaaacgcatg aggagccgcc gctaaaactg caatgtccca agaatgggaa
      541 atactacagt tttcaggaaa ttcaggcttt ttacggaatc agccgcccaa cacttgactc
      601 gtgggttctc aaagaaggaa ttccaacgat gaagttggga agggtaaaac gcatccaaaa
      661 agatgctttc gatgaagttc tgaagtccaa acgtaaagta gaggaggatg tataatggct
      721 agcattgtca aacgcaataa ccgatactgc gttgtctata cctacaaaca cactaatgga
      781 acgctaaagc agaaatggga aacctttacc gaccttgcag atgccaaaaa ccgtaagaaa
      841 gaggtcgaat acaaagaatc cgtcggtacc tttgtggcac cccagtgccg aactctaaaa
      901 gacctgctta aagaatacgt caccctgtac ggaagaacca aatgggcact ttcaacctat
      961 caatcaaaca ctgcactgat ttcaaactac atagaaccgc ttatcggctc aatgaagctg
     1021 caagatctca ccacccgcgt aattgaggga tattatcagc ggctgcttaa atacgaagcc
     1081 gttgacccta tgtgtggaaa gcgtcagcat caatacgttt ctcccggcac agttcgttcg
     1141 gttcacaaaa ttttgagaag tgcctttgag caggccgtaa agtgggaact tatggaaaag
     1201 aatccctgta tttatgccac cttgcccaaa tacactgcaa agaagcgtga tatctggact
     1261 gccgagaccc tgttccatgc cttagaagtc tgtgatgatc cacggctccg gctttgcatc
     1321 aacctctcct tctcttgttc cctgcgctta ggggaattgc tcggtctgac atgggattgt
     1381 gtggatatct cccccgaaag cattgaagcc gggcgagcct ccatttacat aaataaggaa
     1441 ttgcagcgtg tagatattgc ttcgctgaac gccttggaaa ataaaaatgt catcactcgt
     1501 tttccctcgc tgagttcccg ctgcaccacg gtccaggttc tgaaaagccc taaaacagac
     1561 agcagtatcc gaaccatttt tctccccaaa acagttgctg aaatgcttgt ccactataaa
     1621 gccgagcagg acatgacaaa ggacgcactg ggtgcagagt atgcagacta taatctcgtg
     1681 gttgccggcc cgctgggaat gccgaccgaa caatccacca ttaacggtgc attgaagcag
     1741 ctgattgagg aaaacgatct ccccaaagtg gttttccaca gcttccgaca ctccagcatt
     1801 acatacaagc tcaagctgaa cggcggcgat atcaaagccg tgcagggcga ttccggtcat
     1861 gcacaggctt cgatggtcac cgagcagtac gcacacattc tggatgatga tcggcgtttg
     1921 aacgcacaac gattcgacga tttcttctat cagcatcatg gtgcagagcc tgaagtcctc
     1981 cctcgcgcag agcaatccac tcccaaagcc agtcccgtcg atacagatgc ggcagcagct
     2041 cttgctaaac tgctggctga cccctctatg gctacactca ttaaaaatct tgcaaagaac
     2101 ctataagcct ctatacataa acagcggcag ttttccgtat atggaaaact gccgctatct
     2161 gttttaggat gatcccgttt ctttgtgttt tttcgcctta gcttcctcta tgtccatctt
     2221 cagctgatgt gtaagctgtt ttacataatc gagtgaaact tcatagcctt ctgcgatttt
     2281 ttctgccggc atcccatcaa ggatacggga gatcacaatg ttctgagtgg cttgttttct
     2341 cgactctgca atcatttcct caatagaggg gcaatgttta ttatgcatcc aattcaagta
     2401 atccaagcgt tcagcttcta tccgcgatag aagcctgact gcatagaaat tgtcatctac
     2461 ctcgaagcga acttcctctc cgggtttgac ttcaagtttt tccagaaccg catctgggag
     2521 ttttacagtt ccatcttcac cgactacaat attaaatttc ttttccatcg cacactccta
     2581 aaatcacgca aagttccttg catccagtgc ctttactccc gtaagaatgc cataagcccc
     2641 ttccttttac aagacaatgc gcatttccat tcgcactgtt acatagacca gagttttctc
     2701 gcagaaagct ctggtctttt tctattgaaa acatttgtcc atcacaccag ctacggctgt
     2761 atggtctggt tcacgaatgc taaacaagca aatatccgta gggaaacgta cacttcccag
     2821 ctaatgaa
//
LOCUS       DAWEJW010000195         2799 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_9165_length_2799_cov_62.070335, whole genome shotgun sequence.
ACCESSION   DAWEJW010000195 DAWEJW010000000
VERSION     DAWEJW010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9165_length_2799_cov_62.070335"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          774 a          703 c          596 g          726 t
ORIGIN      
        1 gctttggctt ttccgccggg aaaacaagta tatactttca tcttttccat ccttttctac
       61 tgtaatttct attgtggttt taaaatatga taattttcat cccacatgtt ttcatcatat
      121 tacgactgga acaattttgc aactgataag aatccggcat taaccctctt tatcgtgcag
      181 aactttccat tgccttcctt tcattcatcc tttaatatag taaagtatac aacaaataaa
      241 cgaattacaa tgagaaggga gcatatttat gaaaaaattc aactccttcc gtaatcgcca
      301 gaacggcttt gaagatttag gttccggcac cgcggaagca gcaaaaacga aaaaaagctg
      361 gtggaaagtc cccggcatca ttcttgtgat tctggtaatc gctatttttg cttttaattc
      421 tacttaccag atccgtgaac aggaacaggc ggttctgatc actttaggcc aggccaaagc
      481 ggtaacggat cccggacttc attttaagat accgttcatc cagcaggtcc gcaaggtgaa
      541 taccaccatt cagggatttt ccctgggata tgatgtagat tccaattctt cggaaaccga
      601 cgattctctc atgatcactt ccgactataa ttttgtcaac gtcgatttct tcgtagagta
      661 tcgtttcagt gacccggtaa aagctgtcta tgcatccaag gatcccgtac tgatcctgcg
      721 taacatcagc cagagctgta tccgtaccgt gatcggcagt tatccggtgg acgatgtcct
      781 gactaccgga aaaaatgaga ttcaggccgc tattaaagaa atgattctgg agcgtctgag
      841 cgaacaggat atcggtatcc agctggtaaa tatcacaatc caggattccg agccgcctac
      901 ctccgaagtc atggaagctt tcaaggccgt ggaaaccgcc aagcagggaa aagaaactgc
      961 cctgaacaat gccaataagt accgcaatga acaacttcct ctggcgcagg cacaggccga
     1021 cggtatcatc aaagacgctg aggcgcaaaa ggccgagcgc atcaatgagg ctactgcaca
     1081 ggttgcgcgt tttaacgcta tgtacgagga atatatcaag aacccgaccg tcaccaaaca
     1141 gcgtatgttc tacgagacca tggaagaagt actgcctgac cttaaggttg tcatcgaaag
     1201 ccccaacggc aatacacaga ccttctatcc tttggaatcc ttcacaggta ccgcttccgc
     1261 ttcttcggag agcagcacct ctgaagctgc acaataacca ttgtctacag aaaggcagtt
     1321 ttataaatat gaaaaaagtg aaaaatatat ctctgattct attagtactg gcattgttgg
     1381 tcatcggatc ctccagcatt gtcgttacca acgaaaacga atacagtctg atccgacagt
     1441 tcggtaaagt agaccatgta gtgagttctg caggtgttac ctttaagatt ccttttgtac
     1501 agagcgtcga taagcttccg aaggagatcc tgctctatga tctgtcttcc tccgatgtca
     1561 tcaccagtga caagaagacc atgatctgtg acagttacat tttatggcag atcaatgatc
     1621 ctctgaaatt tgcccagacc ctgaactcct ccatctccaa cgcagagagc cgtctggaca
     1681 ccatcgtata taactctacg aaaaatatca taagcagtac gacccagaac gacgtcatct
     1741 ctgggcgtga cggagagctg gctgcagcca ttatgaaaaa cattgggaac accactgagc
     1801 aatacggcat cgaattgctc tccttcgaaa ccaaacagct ggatctgccc agtgacaaca
     1861 aagccgctgt gtacgagcgt atgatctccg aacgtgaaaa catctccgca acctataccg
     1921 cagagggaag ctctgaggca cagaagatcc gcaataccac agataaggaa gtcaatgttc
     1981 tcctgtcgga ggccaataaa aatgcggaga ttctcattgc cgagggcgaa gcggaataca
     2041 tgagaattct gtcagaagct tattccgatg aatccaaaca ggatttctat tccttcgtca
     2101 gaagcctgga tgcactgaag gcttccatga ccggcgataa taagacggtg atcctgtctc
     2161 cggattcccc gatcgcacag attttctacg gaagataaca taaaggccgg tgtctgcaat
     2221 attcttgcag gcaccggctt ttttatgcca gaatctcctc aattctgcgt ctctcttctt
     2281 cactgaatgc ggttgcatgt acaataccga tattatccag gatctgtgag ggcttagatg
     2341 ctccgatcag aacactggta atatccccat cccttaagat ccacgaaagt gccatctgtg
     2401 ccagtgtctg tcctctctgg gctgccagat cgttcagtgc acgtaccttc ttcaatgtct
     2461 cttccacgat cgccgcttct ttcaggaatc tgccgtcggt acggacacgg ctgtcctcag
     2521 gaataccgtg gagataacgg tcggtcagaa gtccctgtgc cagcgggctg aacgtaatga
     2581 tgccgatgcc atgggcagcc gcataatcct tcaatccgtc cttctcaata tctcttacaa
     2641 acatggaata ttttctctgg ttgataataa acggtgtccc cagctccttg aacagttctg
     2701 ctgccgcaat ggtctgctcc ttattgtagt tggaaattcc cacatacaat gccttaccgg
     2761 atctgacaat gccatccagt gcacgaacgg tctcctcca
//
LOCUS       DAWEJW010000196         2755 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_9335_length_2755_cov_64.207778, whole genome shotgun sequence.
ACCESSION   DAWEJW010000196 DAWEJW010000000
VERSION     DAWEJW010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9335_length_2755_cov_64.207778"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          794 a          612 c          691 g          658 t
ORIGIN      
        1 attgcactgg cagatgtgat cctgaacaag tggagagaat atacggatga agaggcattt
       61 atctatgcct acaccgataa cgagcctcat aacaccatca ctcccattgc aagaaagaga
      121 ggagacaagt tcgaactgga tctggtgctg cgtaacaata tcaccacaga ggagcatccg
      181 ctgggagtct atcatcccca tgcaaagctg catcatatca agaaggagaa catcggtctg
      241 atcgaggtta tgggtctggc cgtactgccc gcccgcctga agggtgagat ggcgcatctg
      301 aaggaagcaa tcctcgccgg taaggactta agagccgatg aagaactggc aaagcatgca
      361 gactgggtag atgaattccg accgaaatac gatgctatca ctgcagagaa tatcgacggt
      421 attgtcgaga aagagatcgg tctggtattc atgcaggtat tggaagatgc tggtgtctac
      481 aagagaactg aggaaggtca gaaggcgttc gaccgttttg taaagagctt gtaataatag
      541 ttcagccata gaatgacaga accggcgatt gggtgtttgc acacaaagcg acggagagtg
      601 tgataacgcg actttgcgaa tggcgcgggc tgaacgtttg ttgtataata tttgttgcga
      661 ggaaaaggaa ttgtgggaca caattcttca gactgtagac aaagctcctt cctgtgaggg
      721 agctttgtct acagtctgtt tatttaccgt tatcttctct tttccgaatc atgcctctta
      781 ctttttatag taccatcttc ctgtataggg tgtaagtcgt cactgatggt tcgtataaat
      841 aattttacgc gtttttgctg tattttttaa ggcgcaagat ttgtataatt ccgataattg
      901 tggtatactg taggaaaaaa ttttcgtaac acatcagttc accatgtatt acagaaaaga
      961 ggttatattt caaatgaaaa agcaaagcaa agtaaccagt cagtctgcca tgctgcagca
     1021 aaacacaaga tcaacctatc ggatactgat catcagcatc gttatgctga tcctgttcat
     1081 cggatccaac atgtatctga gccggatcaa ttcccagcag ctggaagcta ccatgtatct
     1141 gaaccagtat cgtctgggtt ccaagacgct gaccgctgcc gtacagtctt acgcagtcac
     1201 cggagaccag acctactatg acaattatat gaaggaatta aacgaggaca aaaatcgtga
     1261 cattgcctgg gaaggtcttc agaaagacgg tctgaccgac aatgaatggg cactgttaaa
     1321 ccatattgct gaaatgtcca acggtctggt tcctctggaa gaagaagcca tggataaggc
     1381 cggttccgga gatacacaag cagctatttc ttatgtattt ggtgaggaat atgaatctac
     1441 cgtacaggag atcactgcaa ccacagataa ttgtatcaat gatatccagg cacgtatggc
     1501 tcagaagcaa aatactctga atctgataat gatcactacc atggtgattt tcattctctg
     1561 cttcctgacc attgcccgca agattgtcac cacactgacc tttgcaaaac aggaactgct
     1621 gattcccatt gtaaaagtat ccgagcagat gaaagtactt gcgcagggac attttgacag
     1681 ccgtctggat ctgccggagg atgacagtga agtcggcatc atggtgcagg ccgttcattt
     1741 catgaacgac aactttacca agatgatcac cgagatttcc gagatcttag gtcagatggg
     1801 tcagggcaat tactgtgtag agcctacgga agaatatgtc ggtgactttg tacagatcaa
     1861 ggattccttg gtgaagatca ttgccgatat gaagaagaca ctgtccacca ttcaggtcag
     1921 cgcacaggag atcgatggtg gttccgagca gctggcacag gcagctaccg atctggcaga
     1981 aggttgtacc gcacaggcct ccaagatttc cgaagcatcc cagatgatcg acgccatggc
     2041 aaagagtatt gaagaaaaag ccagagttgc acaggagacc gcagatatct ccaagcagtc
     2101 cgcacagacg gttgctgatg gcaatgccaa gatgcaggaa ctgaaagtcg caatcggtga
     2161 gatcagtaaa tgttccgagg agattcgttc tatcattcag gtcattgagg atatcgcttc
     2221 ccagaccaac ctcctgtcac tgaatgcctc catcgaggct gcccgtgccg gagaagcagg
     2281 ccgcggattt gctgtcgtag ccgagcaggt caagaatctg gcagaacagt ccaccgaggc
     2341 tgccggagag accacaaagc tgatcgagag caccatcgat gctgtcaaca agggaatcgc
     2401 cattgcagaa gaaaccgaag ccagcatgga tcaggtaatg aaagaagccg aggcttctac
     2461 caagcgtatg gttgatatgg cacaggccct tcaggctgaa gtgtccagcg tccagcagat
     2521 tgatgaaaac atcgctcatg tggccggaat cgtggacaac aactccgcat cttcgcagga
     2581 gaccgccgct gtcagcgaag aacagtccgc gcaggtacat actatgttac agctgatgca
     2641 tcagttccag atctagtttt cagaatgata aagcctgagt gcttctaaag aagcctcagg
     2701 ctcagactgt agacaaagct ccttcctgtg agggagcttt tcttgtgtca tgact
//
LOCUS       DAWEJW010000197         2584 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_10029_length_2584_cov_61.274417, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000197 DAWEJW010000000
VERSION     DAWEJW010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10029_length_2584_cov_61.274417"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          732 a          532 c          694 g          626 t
ORIGIN      
        1 caaaaaggca tatgacatga ctccggtaca gtatcgaaaa cagagcgcgg aataaagtac
       61 gccgtggaac tgcgccatgc acattcgtag acgtactgcg tctacttcat gtacggctgt
      121 cgccgtataa atccaaaaag aaaagcacgt cctcgtgagc aagctcccag ggcgtgtttt
      181 tctttttgaa tttgcatggc tcgtagcttt taacataatg acaggtttgg aaaacataaa
      241 taaatagaaa aaaccgtttt tttttattat gatgaaaaca tagagcaaga gaatttgcag
      301 tctggcattc cgccgaagcg taacgaaaga aaacataaat gtaagaggct tacatcgtaa
      361 gcagacagga gtagaaaaat ggaaatgaaa gagacgattc tgaaaacttt cgcagaagta
      421 tttggagatg cagagggcac caaggcttat tttgcaccgg gacgtgtgaa tctgatcggt
      481 gaacataccg actataacgg cggacacgtt ttcccctgcg cactgaccat cggtacctat
      541 ggtgtggcaa gaaagagaaa cgacaataag ctgcgtttct attccatgaa tttcggacat
      601 ctgggagtga tcgaatcttc tctggaggat ctggtaccca gcaaggaagc aggctggacc
      661 aattatccca agggagtgat ctgggctttc ggtgagaagg gcatgaaagt gacttccggt
      721 atggatctgc tgctgaacgg taatatcccc aacggttcag gactttcttc ttccgcttct
      781 gtagaagtgc tgaccggcta catcctgagg gatttcttcg gctttgatgt gaccaatcag
      841 gatctggcgc tgatcggaca gttctccgag aacaaataca acaaggtaaa ctgcggtatc
      901 atggatcagt ttgccattgc catgggtaaa aaagagaacg ctattttcct ggatactgcg
      961 actctggagt atgagtatgc accgatccat ctggagaatg cgaagatcgt gatcgcatgt
     1021 agtaataaaa agagaggtct gggagattcc aagtacaatg agcgccgcag tgagtgtgag
     1081 actgcactgg cagagcttca gcaggtagtg aagatcaaga cgctgggcga cctggatgag
     1141 gagacctttg agaagttcaa ggctattatc aagagtgatg tccgcagaaa gcgtgcaaag
     1201 catgcagtat atgagaatca gagaacgatc cgtgcggttg aagcgctgaa gaacaatgat
     1261 gtgaagctgt ttggtgagct gatgaatgca tcccacgtat ccctgcggga tgattatgaa
     1321 gtaaccggta ttgagctgga tactctggta gaggaagcat ggaaggtaga tggtgtcatt
     1381 ggatcccgta tgaccggtgc cggctttggc ggatgtaccg taagcattgt gaaggatgaa
     1441 gctattgata cctttatcga gcaggtaggc aaagcataca aggaaaagat cggctatgcg
     1501 gctgatttct atgtagtaga gatcggtgac ggcccccaga cactgtaagg agaaaagaaa
     1561 atggatattc aggtattaat tcgtaaattg gtatcctacg gagtacagac aggactggtt
     1621 ccggaggaag atgtgatcta cacgaccaac cgtctgttgg agctgtttga actggatgaa
     1681 ctggaagagc gcgacgatgt gaccatgcgt gaggatgaac tggaagaggt tctgaagggc
     1741 atgctggatt acgcttatga aaaaggcatt ctcaaggaga acagtgtggt ataccgtgac
     1801 cttttcgata cgaagatcat gggactgttg atgcccagac ccagtgaagt aatccgtcat
     1861 ttccacgaac tgtacgagca ggtatctccc gaggcagcta ccgactatta ttacaaatta
     1921 agcagagatt ccgactatat ccgccgttat cgtatctgca aggatatgaa atgggtagca
     1981 cccaccaaat acggcgatct ggacattacc atcaacctgt ccaagcctga gaaggatccc
     2041 aaggctattg ctgcagcgaa gttggcaaaa caggccggtt atccgaaatg cctgctgtgc
     2101 cgtgaaaatg aaggttatgc aggccgtgtg aatcatcccg cccgccagaa ccaccgcatc
     2161 attccggtga ccatcaatgg cagccagtgg ggattccagt attctccgta tgtatattat
     2221 aatgagcact gcatcgtatt taactctcag catgtgccta tgaagattga gcatgcgacc
     2281 ttctgtaagc tgtttgattt cgtaaagcag ttccctcatt attttgtagg ttccaatgcg
     2341 gatctgccca tcgtaggcgg ttccatctta agccacgatc atttccaggg tggccattac
     2401 gagtttgcca tggcgaaagc tccggtagag agaacgttct ccgtagccgg tttcgaggat
     2461 atggatgccg gtatcgtggc atggcctatg tccgtgatcc gtctcagcgg tacggatact
     2521 gaccgtatca ttgcactggc agatgtgatc ctgaacaagt ggagagaata tacggatgaa
     2581 gagg
//
LOCUS       DAWEJW010000198         2401 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_10871_length_2401_cov_51.504263, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000198 DAWEJW010000000
VERSION     DAWEJW010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10871_length_2401_cov_51.504263"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          686 a          488 c          512 g          715 t
ORIGIN      
        1 gccagtattt gtttctatta tctgttattc cacggaagca gtatcaagac aggctttcat
       61 acgatcatcg ggattctgat gccggtggta ttcggactgg tgacggcata tctgctgacc
      121 ccggtactta attttataga aaccaaactg ctatttcctt tgtgccggaa aatgaaggtg
      181 aaggagagca ggaagcgcaa ttccattgtc cgtggtattg gcatactgat cacggcattt
      241 ttgttcgtca ccctgattta tagtctggtt gccatgatgc tttcccagat tgttcccagt
      301 attgtaaata ttgcttccaa ttttgacacc tacatcagca attttacaaa atggatcagc
      361 aagcttatgg atgacaatcc ggagctgggc agtcagatca cttttctgat tgataagtat
      421 tccgtagaac tggagaattg gttaaataac acagtactga ccaagaccag tcaggtgatc
      481 atgacagtat ctttgagcgt aatcaatatt ctgaagagtc tttgggattt tatcatcgga
      541 ttcgttattt ccatttatgt actggcaagt aaggaaaaat ttgccggaca agcgaaaaaa
      601 atggcctatg ctttctttga acagaaaaca gccaaccgcc tgatcaagag ttttcgcttt
      661 acccatagca ctttcatcgg atttatcggc ggcaagattg tagattcctt tatcatcgga
      721 tgcctgtgct ttatcggaac cacgttattg cagacaccgt atgcggcact ggtcagcgtg
      781 attgtgggtg tgaccaatat tattcccttc tttggtcctt atctgggagc tattcccagt
      841 gccatcctga tccttgtggt ggatccgatg caccctttaa actgtgttta tttcgtcctg
      901 ttcattctgg tgttacagca gtttgacgga aatttcctgg gacccaaaat tctgggcaat
      961 tccaccgggc ttaccggttt ctgggtgatt ttcgccatca cggtattcgg tggattattc
     1021 ggtattctcg gcatgatcgt cggggtgcct atctttgcag tgatctatgc ggctatcaag
     1081 gcagtagtta atacgttcct tcggaaaaag gatctgccgg tagagacaga acaatattct
     1141 tatctgaaaa atattgatga agagggattc catacactga attccagact gcaaaaggat
     1201 catgagctta aagatcaaac tgatttcagc caggataaaa aagatcaaga agagaaaaac
     1261 gtaggagaga agtagacaaa tgcgatttgt aatacaacgg gtacagcatg cagaggtaaa
     1321 agtagatgga gaggtaacag gcagcatcgg caaaggtttt ctggtactga tcggcgtggc
     1381 cgaaacagat accagagaga ttgccgacaa aatgatcaag aagcttctgg ggctgcggat
     1441 ctttgaagac gctcagggca agaccaatct ggatctgcat gcggtggagg gcgaattgtt
     1501 actggtgtca caatttactc tgtatgcgga ttgcaaaaaa ggcaatcgtc catcctttat
     1561 caaagccggt aatccggata tagccaatga actatacgaa tacattatcg acagatgccg
     1621 tgaaagcatc gccaccgtgg aaaaaggaat ctttggcgcc gatatgaaag tatctctgtt
     1681 aaatgacggg ccttttacca tattgctgga ttccgaggaa atctgttaaa aatagctttc
     1741 gaaaggcgta ttataggatt atttctaaac acaactattc gttaaacttg tgttttgcgc
     1801 atagttggtt cggtgactgc tatcttagta agggctgatt tgttccttaa cagaccggaa
     1861 tgtgttggct ctgcatgggc ggtacacatt ccggttgaaa aatgttgaag ttatctattc
     1921 gcataatcaa tcaaaatctt tttgcaatta ctgcaacgat aagcttcaca atgagggatt
     1981 ccgcctgaac ctttactcaa ttccagatcg tccgattttt taatgcgaaa aacttttgtc
     2041 agattatcaa ctgcaaaagt aatgtctcgt gagctcatta ggtatcctct tatcatttct
     2101 tcaccacaat acggacattt cattctactt tacctcctat aaatatcgat ttctttctct
     2161 gtgacttccc aataaacggg aatttctcac ttcttcattt taggagcaat aagcagcact
     2221 actgtcacaa gaaaaaatac accatacagt aaccacgtaa ttgtattgtt ccaagaaaaa
     2281 ctcttttctg gatggttcaa tgcaaacatt ataaatacaa aagctacaat cagcattatc
     2341 acaccgataa tccttgatat actcttattc atgtaaagca cctcctcgtc aaacttgaat
     2401 t
//
LOCUS       DAWEJW010000199         2342 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_11205_length_2342_cov_35.743332, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000199 DAWEJW010000000
VERSION     DAWEJW010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11205_length_2342_cov_35.743332"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          696 a          423 c          618 g          605 t
ORIGIN      
        1 cactgatctt cgtgctgatc cagatcgttc aggtggtgtt tcccatcata ccgggtggca
       61 tcagctgtct ggcaggagtg ctgctctttg gagtggtgcc gggatttttc tataattata
      121 taggaatcag tgtgggttcc tgtattgctt ttggaatagc aagaagtctg ggaagatcgg
      181 tgctgtacaa gatgtttccc gggaaaatga ttgaaaaata cctgacatgg acggagttga
      241 aggggagatt tttgaaatta tttgcactgg caatcttcct gccggtggca ccggatgatt
      301 ttctctgtta tctcgccgga acgacgaata tgacatggaa acagtttgtg acagtcatct
      361 ttctggggaa gcctttctcc attgcgctgt atagtctggg actgacgaca ctgttccagg
      421 tgattttcca cctggtatag gagagtatac ttatgaaaat ttatatatat aaaggcggac
      481 tttcactggt ggcaaaaagc ggcgtcggca gcgccatccg ccatcaggag aagatgctgc
      541 gagcagcaaa agtgaccgtg acggacgtct ggaaagaggc ggatatcgta cagatcaata
      601 ctgtattgcc tgattcgccg ctgatggcac ggcgggcaag acggcagggg aaaaaggtcg
      661 tctattacgg acattccacc atggaagatt ttaagaattc ttttatcggt tccaatctgg
      721 cggctccttt attcaaaaaa tggatccgcc attgttatag acagggagat gtggttatca
      781 caccaacgga atactcacgg aaattgttaa tgaaatatga tctgcatcgg gagatttatg
      841 cactgaccaa tggtgtggac actgaatttt tccacaaaac agagcaggca ggtggacgat
      901 tccggatatt ttttcatctt cctgtggata aaaaggtcgt gatatccgcc gggcatctga
      961 ttcacagaaa aggaatcgag gattttctgg aaatggcaaa aaggatgcca gacattattt
     1021 ttctatggtt tgggggcggg aacaatgcgt tgattccggc agagatcaaa aaggctgtgg
     1081 cagagaagcc ggaaaatgtg atctttgccg gtttccaacc gtccgggatc ctgcgggatg
     1141 cgtactgtgg tgcggatgca tttgcttttt tcagttatga agagacggaa ggaatcgtgg
     1201 tattggaggc attatcctgt gagattcctg tgatcgtgag agacattccg gtgtatgaag
     1261 ggtggcttac cgacggcgag caggtgcgca aagccagcaa tctggattcc tacgagcagg
     1321 cagtccgaga catcttgtat gaagaaccgc gggaaaaagt aagacgcaga atccgggcag
     1381 gcagggcact ggcggaggaa cacagcatgc gggcgacagg tgagaaactg tatcagatag
     1441 agcagaagct tttgtagaca tccctcttgc aaattcagag tctttatcat aaaatgagag
     1501 taccttacaa aggaggtgct tacgatgaac cttgtaatta ctatcagccg aagattcgga
     1561 acaggagcca gtctgatcgc acaggagttg tctgagaagc tgggggtgcc ggtatatgat
     1621 aaggcgtaca tagagcatga gctggatggt gacagctatg ctacggaagc ggaggtcatt
     1681 aaggaattgg cggaacatcc ctgtattatt ctgggacgtt gtgcatccga gatcctgaag
     1741 gatcagccga atgtatttaa cgtgtatgtg tgcgcggata aggaagatcg tattgaacgt
     1801 atcatgaaga aggaaagtct ttcccacgat gaggcgaagg aaatgcttga gaaaaatgat
     1861 gcagaacgcg ccgcctatta ttatgagaat accggtaagg tgtggggcga tgtgaacaac
     1921 taccacatga tcctggatac caccaagctg ggaatcgaga actgcgcgga tattctgatc
     1981 cggtattttg aaagagtgga aattatttga cagcccggaa tgcgaatggg cgttacgatt
     2041 tcgagagtat gcagtacgaa gaaatcggtt aggatataaa tagggaatag attgtaaaca
     2101 gatgtctcac acggacatct gtttttctat gaaaaacatc ttaaacacag taataaaatt
     2161 aatcaattat acaacgttat aaaattaaaa gtatgatttt aaatataaca aaaaatgata
     2221 tatacaattg aaaattttaa tcaaataata aaacggaaca atcaccgaat aaaatgacct
     2281 ttttcttgaa tttatagata tatgacaaaa atgtcacatt aaaatacatc aattaacaag
     2341 at
//
LOCUS       DAWEJW010000200         2253 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_11740_length_2253_cov_55.972247, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000200 DAWEJW010000000
VERSION     DAWEJW010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11740_length_2253_cov_55.972247"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          606 a          601 c          492 g          554 t
ORIGIN      
        1 gggagacctt acatcgagga aggacattcc gtccgccatg ggcgttcgtg gatttatttc
       61 actaactctc aatttagtac tcttccgcaa tatcaatctc ttcctgctcg gcagctgtag
      121 tatctgcaga gtacggggtt ctgtggaggt atgccagcca gtagccgcat cctacgatcc
      181 cagcaccacc aacgatgttg ccaagggtaa taggcagcag ggcttttacg aggaaattga
      241 accaggtcag gctctctgct gcaatgccgt agacctgcgc tgtcagaaga ccgcatacac
      301 cgaaatacat atctgcgata ctgtgctcga agccgcataa tacaaatagc atcacgggcc
      361 agtaactggt cagaagcttg ccggagaccc gcttcgcagc aaaggacatc cataccgcga
      421 tgcacaccag aatattacac aggatacctc ttacgaaagc ctcacccaca cttaaattcg
      481 tccgggatgc cgcggcatta acaatggcct gtgccagctg ctctccgaaa agatccggtg
      541 tccgtcccac cactaccagt actgccacaa aagccgcacc gaggaagttg ccgatgtaaa
      601 caaacagcca gtttttcagc atcttccata ccgtgacttt cttctccagc actgccagta
      661 tgatcagatt gttgccggtg aatagctcgc tgcccgctac cagtaccata gccatacccg
      721 ctgggaatac cgcagcgcct accagcttcg ccaccgatgc gatgccgatg gtgctggatg
      781 ccgtagtcga agcaattcct gcaaaaccga tgaacatacc ggcaaaaaat cccaacagaa
      841 tcgtcttaaa gatcgacaga tttaccttgt ggatcccgac ttctatataa tttcttgcaa
      901 tttccaaagg tgaatgcata accaaattcc tccttatcct tttttaacag ttccatcttg
      961 ccatatttcc aaaactcttg caagcaaatc cgaaaagaat atttccctct cttgattttc
     1021 gcacgttaat ttgctatagt gtttaaagga caaacggaca tcttccgact ttgtcatact
     1081 atttatttgc agcaaataac agaatcgagg aaatctacat gagtactctt acatttggtt
     1141 ttatcggtct gggtctgatc ggcggttcca tcgcccgggc gatccgtcag aatctccctc
     1201 agtctcagat cattgcatac gatatcaatg cagataccct gcgggaggct gcccagtgtg
     1261 gtgttgccaa cgtgatcacc acaaggatcg atgcttcctt ctccgactgc gattatcttt
     1321 tcctctgtgc tccggtacag aaaaatgacg agaacctctc agctgtaaaa gaaattctct
     1381 caccaaagac actgctcact gatgtgggaa gtgtcaagag tgagattcat acagagatca
     1441 aaaaagccgg tctggaagga cagtttatcg gcggtcatcc catggccgga agtgaccgta
     1501 taggctttat caattccaag gccgttcttt tggagaatgc ctattacatt ctcacgccca
     1561 cggattccgt tccggaaaac aaagtcacgg attataaaaa tcttgtacag cagttgggtg
     1621 ccatccctat gattctggag ccggcacagc atgattacgt tactgcagct gtcagtcatc
     1681 tcccccatat cattgccgca tctctggtaa atctggtgcg ggacaaggat tctgcggacg
     1741 gactgatgaa gatgatcgct gccggaggtt tcaaggacat taccagaatt gcctcttctt
     1801 ccgctgcggt atggcagcag atctgcctga ccaatacaga aaatatctcg acactgttgt
     1861 cctcctacat tgcttccctg caggggattc agcaggaatt aaatgccaaa agcggtgatg
     1921 acctgtatga actgttcgac agtgccagaa tctaccggga ttccttcatc aacactgcca
     1981 gcggcccgtt aaaatcttct tatgcaatca ccattgacat tgccgacgaa cccggtgaga
     2041 ttgccgccgt ggctacgatt cttgccctga aaaatatcag tatcaaaaac atcggcatcg
     2101 tccataaccg ggagtacgaa ggcggcattc tgcgcattga attctatcag gaagaagcca
     2161 tcgagagatc cacaaagatt ctttgcgaaa agggctacag tctgcacaac aggaagtaga
     2221 aaccctgtac ctggctcatc gcactacaca aaa
//
LOCUS       DAWEJW010000201         2253 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_11741_length_2253_cov_44.467698, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000201 DAWEJW010000000
VERSION     DAWEJW010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11741_length_2253_cov_44.467698"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          634 a          497 c          603 g          519 t
ORIGIN      
        1 gtgtggcatt tgttttttat gggtgtttat cgagaaaatc ataagtgcga agcacatgct
       61 ctgcgaagca gagcgatttt cgaagatata aacgtggatg tgcaaagtac ccgatctcca
      121 agcttgcttg agaggtcggg tattttttat gtcacggata tggcgaagtc cagcggatat
      181 cgcatgaatt gcgaagcaat ttatgcgata agccgctgct gcgtcagatc cccttgactc
      241 tcccccaaaa acctcttaaa cttatctcaa acacatcaaa gaaagaggta tacccttatg
      301 aatcgttttg aatacatcgt agaaagcatt gacggcgatt atgcccatct tcgcagaaca
      361 gacattgaat ccgatgaatt aaagctggtg gcccgcgcgc tgcttccgcc cgagatcatg
      421 gagggcacga agctgttata cgagtggatg cagtattcca ttatggaata attatgccaa
      481 attttgaaat gtccggagaa aacgcaatat ttgtcataaa ataaaaggga aactggcacg
      541 acaggagagt tggtaaagaa tacacttata gtatgagcgt ggtacacgca gctttgcgca
      601 aatagaaaga ctaatacatg taaaaaggag actccctatg agtgaaccta gaacttatca
      661 taaccctgta caacgcggtt ttttcccgga tccttcggtg atccgtgtcg gtgacgatta
      721 ttatatggtg aattccagct tccagtattt tccggcaatt cccatttccc attcccggga
      781 tatggtgcac tggcatatca tcggccatgc catcacgaac ccggaatggc tggacatcag
      841 tgacatccgg gattcccacg gaatctgggc accggatatc gaatatgtgg acggaaaata
      901 ctatatctat gtaaccttgc gtctgaatgc ggatggcaag cgtgacaata acgtgatgcg
      961 cagacagctg gtgatggtat ctgacaagcc ggaaggaccc tacagcaagc cggtatgtct
     1021 ggaagtggat gatatcgatc cctcactttt tgtggatgat gacggcagca gatatatgat
     1081 catagccaag gcggcacagg cggttcctct gactgcggac ggactggcgg tggctggtcc
     1141 tgcgaagact gcatgggaag gcacaggcga gcgctgttcc gaaggccccc atatcatgaa
     1201 aaaagacggt tactattacg ctatcgtggc tgagggtggt accggctacg gtcatggaat
     1261 caatgtggga agaagtaaga atttctatgg cccctatgaa tgttctccct acaatccggt
     1321 catgcgtcag aaggatccca cagcccccat ccagagagcc ggacacggca aactggtaca
     1381 ggatcagaac ggtcagtggt ggtgttatta tctctgcgga agacctaacg agggcaacta
     1441 tactaccgta ggcagagagt ctgctctgga tcccgtacag tggacggagg acggctggtt
     1501 taccgtcaac gaaggaaaag gtcccagcct gacacagatc gcaccggatc tgccggagtg
     1561 tatttatgag agaaatctgt tcgacgattt taacgataca aagctgaatc tggaatggga
     1621 gttcgtccgc aacccggata acggttcctg gtctctgacc gagcaccccg gatatttccg
     1681 gatctggacc agagatggac agctgaatga gatccgggcg aaaaatacgt tgctgcgcag
     1741 agaacaggaa ttgtcctaca tggcagagac gaagctggag ttctacccgg ataaggacgg
     1801 agaacaggcg ggactgacct gctattacag tactgctact tatgccagat gctccctgtg
     1861 ttatgaaggc ggacgaaaga tccaactggt catcaaccgc aaccacggtg aggaattgat
     1921 agcggaagtg gataatgtga aggagacccc ggtgtatctg aaggtagtgg tggagaagct
     1981 gaccagaact ttctattaca gctatgatgg tcaaaaatgg gaaaaggtcg gagctctgga
     2041 gaactgtatt tacctctgcg acgagggcgt gcccgacgat cccaagcgtc acactggtac
     2101 gctggtaggc atctacgcca acaacggagg ctgcggaagc cgcaaagcag cggatttcga
     2161 ttacttcaaa tatgaggatt agacaaaaat gaaggtgcga ttttggctaa aagtgcgaaa
     2221 aactcgcaac tgtaagaaat ctgtttgcca aat
//
LOCUS       DAWEJW010000202         2223 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            k141_42068_length_2223_cov_86.0000, whole genome shotgun sequence.
ACCESSION   DAWEJW010000202 DAWEJW010000000
VERSION     DAWEJW010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42068_length_2223_cov_86.0000"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          641 a          442 c          591 g          549 t
ORIGIN      
        1 caggtcattc aggtggcaag tccacgtgtt gatgctgtca tttccaaggt atataataaa
       61 tccagaagtg actgcctgga gctgttccgg gcgggaaagg tatatgtgaa cggcaggctg
      121 tgtgaaaata attccaagcc cctggagacg ggggatgtgg taaatgccag aggctacggt
      181 aaattccgca taagtggaga accccatgcc acgaggaagg ggaagcttgc catagaggct
      241 gaggtatatc cgtagcggtt cggtcatgag atgacagttg ccttgctcgt aaggaaaaaa
      301 cgaagacgtg aaggaggtta aacagtgtat cattatactg cgatccaatg gctatttttc
      361 ttttattttt attgcttttt cggctggtgt tttgaatcca cctatgtatc cctgaaaagc
      421 aggaagctgg tcaaccgtgg tttctgccgc ggaccgtttc tgccgctgta tggaagtgga
      481 gccatcatga tgctggtggt gtccatgccg tttcaggaca atctggtgtt agtctatatt
      541 gccggatgta tcggggctac ggtgctggag tatgtcacag gcgtgactat ggaggcgtta
      601 tttaaagtca gatattggga ctattccaag aataaattca atttccaggg acatatctgc
      661 ctgggggcct cgctggcatg gggtggtctg acgatcctga tgacggagtt tatccataag
      721 cccattgaac atctggtatt gtccattccg gacagcatcc tcactccggt gacactggtg
      781 ctgacggcgc tgatcggtgc ggatttcgca ttatccttca aggcagccct ggatctgcgg
      841 gatgtactgg agaagatgga gaaggcaaaa gaagatatgc tgcggatcca gaaacggctg
      901 gatgtcatta ttgctgtgac cagtgaagat ctggagaacc gcagggaagg cttcagtgat
      961 agcctggcac agaagaagga agactttact gcaagtatta ccaccagagt ggaggacctg
     1021 aagtccaaca ttgagggcaa gctggagagc ttccgcactt ccgcacagaa atctcccaat
     1081 cagtatttgg agagcgtgaa gaacgaggtc gccgagttgc ggcagaagta caccagaagc
     1141 gtggcagaca gagagaatgt cagcagctta agagatttct tccagcgtga catgatccgt
     1201 tccaatccga ccatgagttc cgagaagttc aaggaagctc tggaagaact gaaagagcac
     1261 tccaataaaa aaaatgaata aaaagcagaa aaagcatgtc tgtgatgccc gtttttaggg
     1321 gggcggaagg gcatgctttt tttgcacatg gaaaatgaga aaaaaatgaa aaaaaggaag
     1381 aaatagggtt gacaatataa cggggggggg taaactaaat aatggtaaaa ataaaagcca
     1441 taacattata cttaggagga atgggataat gaaaaagaaa ttaattattg ccgcagccat
     1501 tgcatttgca gtatttgcag ttccttacgg cagcagtgca ctgtctaccg tatctgtagc
     1561 aagtgagaat aatacggtgg ttacaccgac ccctgcaccg agcaccaaac cggtagaaga
     1621 ggtaaagaat gcggtagatg ctattgatgc atccaagatt actgatgcgg catctgcaga
     1681 taatgcggca gttgctttga taaagatcgg ttctcaggat ctgaaggaag caatgaatgc
     1741 aggacaggat actgttaatg atgttgcagc catcgaagca gcatacaaaa aggcaaaggg
     1801 aattaaggat actactcttg ctaactccgg cgctgtaaag gctgtaggca ttgttggtgc
     1861 agcatttgtt gctcctgaca caacactgag tgtagaagca ccggcggcta cacctgagat
     1921 tacttcttct acatacgcag taacttctac tcccgtatat gtagaaatca gcctgaaggc
     1981 tggtacttat tcagttgaat ctctgcctat tcccgtagca gttaccattg agactcctgc
     2041 aggtgtagat ggtaataagg cagtgatttt ccactttgta aatggtggac tggaagaaat
     2101 caagcctatt tacaatgctt ccgcaaacac tttgaccttt actgtaaatc acttctccac
     2161 ttttgctatt gcagaagcta ataatactgc aacagcagaa ggtactgaca acgcatttgg
     2221 aag
//
LOCUS       DAWEJW010000203         2180 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_12185_length_2180_cov_69.379765, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000203 DAWEJW010000000
VERSION     DAWEJW010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12185_length_2180_cov_69.379765"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          640 a          471 c          556 g          513 t
ORIGIN      
        1 gaatttatag atatatgaca aaaatgtcac attaaaatac atcaattaac aagataagaa
       61 tatatcttgc ttacagaaat tatgagtgtt aagataatgc aatcagataa gtgaaaatag
      121 taaaaacaac aggttttgaa aataaaaagt aatcatatgg caggaggatt tccatgaaca
      181 gcgcagacat tgcagcaaaa tatcagaaaa cttattttat gccccccgag gtgacctacg
      241 actgggtaaa gaaggacagt attaccaagc cccccatctg gtgcagcgtt gacttgagag
      301 atggtaacca ggctttgatc gatcccatga gcctggaaga taagctggaa ttcttcaagc
      361 tgttggtaaa gatcggtttc agggagatcg aggttggttt tcctgcttct tccgataccg
      421 agtacaattt tatccgtgcc ttgatcgaac gtgacatgat tcccaatgat gtgaccattc
      481 aggtgttgac gcaggcgaga gaacatatca tccgcaagac ctttgaagcg gtaaaaggtg
      541 cgcccaacgc agtgatccat ctatacaact ctacttccgt agagcagaga gaacaggtat
      601 ttaagaaaga taaagaagcc atcaagaagc tggcagtaga cggcgcccag atgttaaaga
      661 atctggcaga cgagaccgaa ggcaacttca cctttgagta cagccctgag agcttttctc
      721 agaccgaggt ggattatgca ctggaagtct gcaatgcagt actggatgta tggaagccta
      781 ccgctgaccg caaggccatc atcaatctgc ccaccaccgt acaggtggca atgccccacg
      841 tatttgcctg tcaggtagaa tatatgcaca agcatttgaa atatcgtgac aatgtagtac
      901 tcagcgtaca tcctcacaat gacaggggct gtggcatcag tgatgcggaa ttcggcgtac
      961 tggccggagc agaccgtgtg gaaggtactt tgttcggcaa cggcgaacgt accggtaacg
     1021 tggatctggt aactgttgcg ctgaacatga tgtgccacgg cgtggacagt ggactggatt
     1081 tctcccatat tatggagatc cgcgaagctt atgagaactt caccggcatg aaggtacatg
     1141 aaagagtgcc ctatgcagga gatctggtat ttaccgcatt ttccggttcc catcaggatg
     1201 ccatcagcaa aggtatggca tggcagaagg aaggcaagac cggcaagcgt tgggatatcc
     1261 cttatctgcc catcgatcct gcggatgtgg gaagagagta cgaatccgat gttatccgta
     1321 tcaacagtgt atccggtaag ggcggcgtag cgttcgtatt gaagcagcag tttggcttct
     1381 cgctgcccgc agccatgaag gaagaggttg gttaccttgt aaaaggtatt tccgacagac
     1441 gtcatcagga attacttccc aaggagatct atgcgatctt cgaggaaaat tatatttatc
     1501 cccgcagcat cttcaacatt ccggagtgcc acttcaaaca ggagaacggc attcaggcag
     1561 aggtgactat cgagcagggc ggagcttcca gagccattac tactatgggt aacggacgtc
     1621 tggatgcagt cagcaatgcc atcaagacat actttggtat tacctacgag ttgtctgtat
     1681 atgaagagca tgcaatttcc agaggctcca attccaaggc tgccacctat gttggtatcg
     1741 tccatgacgg taaattctac tggggcgtcg gcgtagatga agatattatc aagagttcta
     1801 tcgccgcact ggtgtccgcg gtgaataagc tggcagcaga acagcatatt acctccggca
     1861 gagaagagcg gatcgtggag atcatcagtt tcatccagaa gaactatgtg gatgtgacat
     1921 tggatatgct ggtagatact ttccatctct ccaagcctta cctgtccaag tacattaagg
     1981 aaaaggccgg catgaccttc caggaggtcg ttcgggaaga gcgcatgaaa aaagcaagaa
     2041 tgctcttaaa ggagaccaat cagacggtgg agaccattgc agccaacgtt ggttacgaga
     2101 atgtggagca ctttaaccgc ctgttcaaaa aggcatatga catgactccg gtacagtatc
     2161 gaaaacagag cgcggaataa
//
LOCUS       DAWEJW010000204         2139 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_12461_length_2139_cov_56.402111, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000204 DAWEJW010000000
VERSION     DAWEJW010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12461_length_2139_cov_56.402111"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          557 a          597 c          322 g          663 t
ORIGIN      
        1 cggctgctga gttcatccag tcctgataat tgttccataa caaaatccaa atactctttt
       61 gttgatgcca tattattttc ctccataccc ttaggctctc tttatcggat gcctgattac
      121 agtcttcagc ttctccggcg ctaccttcct cgcatcgctc agataaatct cgtggtgcat
      181 tctccgatcc gttatatcca atgcgtatcc ctgttcttcc ataaacctgt gcattgcatc
      241 taccgtaaca ggctcatcgt catacgctcc gatatgcata cactgtacac aaagaccttc
      301 ctcataggaa aaaaactcag ccttagaaaa atcctgcttt ttctttgccg tagctttcct
      361 gacagcccag tcgaaatctt ccctggtaac aaaatccggc agtcgaataa cagagatcca
      421 cttaaaatct tccttacgag cataatcaat tccggatact ccatctcgcc tgccggctcg
      481 gtcggttcgc agcacggcgt cccctagacg ccgctcaccc tgccaccaga atccctctaa
      541 tggcggcacg acataatcga aatatccatc aatctgatga tcttcctttt tgctcatttt
      601 aatcgtaaat gcaattccat acagaagtcc gatagcctgc ttgtattctc cgtctgcatc
      661 atttggattg ccgcttcccc ttaccgctat atagttcatt ttcggaacag ttacgataga
      721 cgatgtgcct tttggcatat aaaattcttt atattccttc ttgtagtcaa aagccatatc
      781 ctttacaccc cctctaagtc cttctcttca taaggcttat tccaggaacc aatctctatc
      841 atatttcctt ccggatcagc aatatagcag gttctctgtc cccaaggttc aagttccggt
      901 tcaagcacag atgttgcacc attttccaca gccttcttgt atgcctcgtc cacctcttca
      961 aaagtatcaa catatagcgc tatttcagaa tgaccattca aacccttgat atactcatat
     1021 cgtcggtttg ttatcttctc aaaatcgttc cgtccataca gtaaaaaaag tgttccgtct
     1081 ttcacaagat aaacattgga agtgttctct tcttccttaa tttcaaatcc gagaacatcc
     1141 ttgtaaaaac gaatcatctt tgccatatca tcaacaaaca accccaaacc gtctaatctc
     1201 attttcctaa tcctccatag atttttaata tttcctctgc attcctgcga ataatgtccc
     1261 gcacttcttc cggctcaagc acctctactt ttgctccaaa tgtcataagc catgtcacaa
     1321 gattatccat atccgtatag tctgctgaaa agagcagcct tccatcgtcc gtttctgtaa
     1381 agcagttcgg tccaaattct tcaaccagcc gccacttcat attcggtgtg aaaagagctt
     1441 ttacatttat cccacccgga aatatcttct catttgacaa atccggcatt ggtacttctc
     1501 tgcgcagaaa tccatggtct gtttcaacaa cacaatccat acggttcagt ttgaacagcc
     1561 tgtaatcttt acgttctaaa caccaccccc atacatacca gctcgaccac cggaaaacca
     1621 gataatatgg ttctaccctt cggttgctct ctccggacgg agcataatac ttaaatcgta
     1681 tgatgcgcct gttttctatt gcactctgta ttacctcgat ctttggagca agagaaccct
     1741 tataccatga agataagtca atcagcatag agtctcttcc actgatgaac tctgatgatc
     1801 cggtctggat tttctccatc agctgactgt agtacctgct tccgctcaca ctgtcaagac
     1861 tccgcaaacc cgcaagaatc atctgcatat ctttggaact caaaatcgtc ctgtccatac
     1921 gatatccatc cataatactg ataccaccac cggtaccctg tgcagttttt ataggaatac
     1981 ctgctttaca aaggtcttca atatcccgat ttattgtcct tcgagatacc tcaaatcttt
     2041 ctgccagttc aggagccgtt gtcttttctt cctgcagtaa gattgacaat ataccgataa
     2101 gtctatctat tttcatcgtt tcttacactc catacatat
//
LOCUS       DAWEJW010000205         2121 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_12594_length_2121_cov_38.242498, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000205 DAWEJW010000000
VERSION     DAWEJW010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12594_length_2121_cov_38.242498"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          486 a          580 c          466 g          589 t
ORIGIN      
        1 atttggcaaa cagatttctt acagttgcga gtttttcgca cttttagcca aaatcaggac
       61 ttcttttttg tctaatcctc atatttaaag tagtcaaaat ccgctgcttt gcggcttcca
      121 cagcctccgt tgttggcata gatgcctacc agcgtaccgg tatggcgttt aggatcatcg
      181 ggaacaccct cgtcacagag gtaaatgcag ttctccagaa ctccgacctg ctcccagttc
      241 tctccgtcat agctgtaata gaaagttctg gtcagcttct ccaccactac tttcagatac
      301 agagcgcctt ccttcacatg gtccacttcc gccaccagct cctcgccgtg gttgcggttg
      361 atgaccagtt ggatcttccg tccgccctca tagcacagag agcatctggc ataggtggct
      421 gtactgtagt agcaggtcag tcccgcctgt tctccgtcct tatccgggta gaattccagc
      481 ttcgtctctg ccacgtagga caactcctgt tctctgcgta acaacgtatt tttcgcacgg
      541 atctcattca actgtccgtc tctggtccag atcctgtaat acccgggacg ttccgtcagt
      601 gaccaggatc cgttatccgg gttgcgcacg aactcccatt ccagattcag cctggtatcg
      661 ttaaaatcat caaataagtt tctctcgtag atacactcgg gaagatccgg tgcaatctgt
      721 gtcaggctgg ggccttttcc ttcgttgacg gtaaaccagc cgtcctccgt ccactgtacg
      781 ggatccagag cagactctct gcctacggta gtatagttgc cctcgttagg tcttccgcag
      841 agataataac accaccactg accgttctga tcctgtacca gcttgccgtg tccggctctc
      901 tgaatgggtg ccgcagggtc cttctgacgc atgaccggat tatagggaga acactcataa
      961 ggaccgtaga aattcttact tcttcccaca ttgatgccat ggccgtaacc ggtgccaccc
     1021 tcagccacga tagcgtaata ataaccgtcc tttttcatga tatggggacc ttcagagcaa
     1081 cgctcgcccg tgccttccca tgcggtcttc gccggacccg ccactgccag tccgtccgcc
     1141 gtcaggggaa ccgcctgtgc tgccttggca atgatcatat atctgctgcc gtcatcatcc
     1201 acgaacagag acggatcgat atcgtccact tccagacata ccggcttact ataaggaccc
     1261 tccggcctgt cggacaccat caccagttgt ctgcgcatta cgttgttatc acgcttgccg
     1321 tccgcattca ggcgtaaggt aacatagata tagtattttc cgtccacata ttcaatatcc
     1381 ggtgcccaga ttccgtggga atcccggatg tcgctgagat ccagccattc cggattcgtg
     1441 atggcatgac cgataatatg ccagtgtacc atatccctgg aatgggaaat ggggatcgcc
     1501 ggaaaatact ggaaactgga attcaccatg taataatcgt taccgacacg gatcaccgaa
     1561 ggatccggga aaaaaccgcg ttgtacaggg ttatgataag ttctaggttc actcataggg
     1621 agtctccttt ttacatgtat tagtctttct atttgcgcaa agctgcgtgt accacgctca
     1681 tactataagt gtattcttta ccaactctcc tgtcgtgcca gtttcccttt tattttatga
     1741 caaatattgc gttttctccg gacatttcaa aatttggcat aattattcca taatggaata
     1801 ctgcatccac tcgtataaca gcttcgtgcc ctccatgatc tcgggcggaa gcagcgcgcg
     1861 ggccaccagc tttaattcat cggattcaat gtctgttctg cgaagatggg cataatcgcc
     1921 gtcaatgctt tctacgatgt attcaaaacg attcataagg gtatacctct ttctttgatg
     1981 tgtttgagat aagtttaaga ggtttttggg ggagagtcaa ggggacgccg ccttatcttc
     2041 gaaaatcgct ctgctttgca gagcacgtgc ttcgcactta tgattttctc gataaacacc
     2101 cataaaaaac aaatgccaca c
//
LOCUS       DAWEJW010000206         2114 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_12632_length_2114_cov_56.100534, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000206 DAWEJW010000000
VERSION     DAWEJW010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12632_length_2114_cov_56.100534"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          449 a          581 c          425 g          659 t
ORIGIN      
        1 gcttcggaag gaatactcct ccagcgttgc gatcatgatc tgactttttc cgtcgctgtc
       61 cgctgacatc tccgggaatc cctcttcgct ctccattttt acccccagga aaattgcctg
      121 gtttgtggca taataatccg tcaaggtaat ggtgtagccc tgatccgata cctgatactg
      181 cgccggttct gtagcttcca cactggtttc cgtactttgg attccaatgg ccccagtatt
      241 gttgtcccca gcctcttctc tctccccggc attgtatttt ccttgccctg ttggtaccag
      301 cgtcacaatt tcttctgtcg gaatctcctg gaaccccagc agctcctgta cccgttctat
      361 cacgctgccg atcagaggca gttcttttgc caacgctgga tttgccccgc aaaatccgat
      421 agcgactacc atcgctgcag ccacggttcc ccactgcaca aatctttttt ttcgcttctg
      481 tctctttttc ttttctgcat tttcccggac gattccgtat gcttcttcta ttttgttgtc
      541 aatcaatgta ctgttcatca tgacccttct cctttcctgc ctacatctgc gatctcagct
      601 gctctctgcc ccggtgaatt ttactcttca ctgtattttc attcatatgt agcagctgtg
      661 cgatctctcc tatggaaaac tgttccccat aatacatctg gaagatcagt ctgctgtcct
      721 ccggcagagt tctcaacaga tcattgaatt ccagatccgt actgaaatca tagaactgtg
      781 aagcttcctc cgccccgttc atgggaaccg tccgactcct gtgccgtctc attgcaatac
      841 attggttgat cagaatacgg atcagccagg ttctgaaata ttcagctctt cgtaaagtat
      901 ccagcttttc aaaagctgaa agtatggtct cctgcatggc atcggcaaca tcctcttcgt
      961 ttcccagata agcgaatgcg atccgtatca tgctttgcct gttctgttcc atcagagcga
     1021 tgaatgcttc tgcgtctcct ttttttgctt ttttcaccag tttctccatg gttcctcccg
     1081 ttcttcatcc ggccggatgc cttgcaaggt attttgtgtt cacatacatt agataagcat
     1141 atcgtatttt cggttgcaga aaaaaaattt ttccataaaa agtttttcca taaaatagca
     1201 aacagcttcc gaagtttcct tcgaaagctg ttcttaaagc tatttgaact actccactac
     1261 taccgtaccg tcttctttta cggtaatatt catgtcatcc ttcacatatt ccagaaactc
     1321 ttcccccagg ctgtcaataa caggcatctg cacgttgtca agccacacat ctgccaaaat
     1381 agctccagct gctgccagag aatcaatggg ctgtgagaaa agcatgcatg ccggctgcct
     1441 gttcatggag caggcacagt aaagtaccag tccgccggtg gtagatccta tggtcgtcgg
     1501 caggcacagg gctttgcccg ccatctgttt accatagagg tcgggattat tctgatctcc
     1561 gcaggttgct tttttatcac cgaactgtaa tgccttctgg aaagatgcca gtgtgttcag
     1621 acccccgtga gataccagtg ccttggctgt tacggttccg gaggctacga ttcttccttt
     1681 aaattctttc atttgtccgc tcctcccttc gtgatctgct ctaagatctc agcatcggta
     1741 taatatcttg cgctggtgta ggtccgcagc ttgttgctgg aagtaatgac cggcatcttc
     1801 tcgctgagag gattattcat atacattaac gggcagatat aggaagtaat gactcccgtt
     1861 gcctttaacg tctccgcata aggggtctcc tgaaattttt tcagtaccgc gggtgccgcc
     1921 gtgaatacgg taggaataca tactctggtt ctaccggctt ctttcaggct ccgagagact
     1981 ttttccgtcc agtcgatgag ctgctgtaaa ctcatatggg gacatcccat gaaacacagc
     2041 ttcggcttgg catccttctt tttccagatc accggatagc tctcatagac cctctgaagt
     2101 tccgcatcat ctac
//
LOCUS       DAWEJW010000207         2103 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            k141_24507_length_2103_cov_150.0000, whole genome shotgun sequence.
ACCESSION   DAWEJW010000207 DAWEJW010000000
VERSION     DAWEJW010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24507_length_2103_cov_150.0000"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          660 a          348 c          508 g          587 t
ORIGIN      
        1 atatcggtgg cattgcagct tactcaggaa aggtggaaaa ataagatgaa ggtaataatc
       61 gtaggaggtg tagcgggagg agctacagcc gcagcaagaa tacgcagact ggatgaacat
      121 gcagaaatta ctgtgtttga aagatccgga tacatatcct atgcgaattg tgggcttcca
      181 tattatatcg gagacgtgat tacagatccg gaagaactta cactacagac accagagagt
      241 ttttttaaac gcttccgtat taacatgaaa attcatcatg aagttatctc aatacatccc
      301 gaaagtaaaa ctgtttcagt gaagaattta gaaaacggtg aaatatttga agaaaactat
      361 gataagctaa tcctctctcc aggtgcaaag cctacacagc cgagatttcc cggagtaggt
      421 attgataaac tttttacact tcgtactgta gaagacactt ttcggataaa ggaatatata
      481 aataagaatc atccaaaatc ggctgtattg gcaggtggcg gttttattgg tctggaactg
      541 gcagaaaatt tgagggagct tggcatggat gttacgattg tccagcggcc taagcagctg
      601 atgaaccctt ttgacccgga tatggcatcc atgatccata atgaaatgag aaagcatggg
      661 ataaaactgg tactgggcta tacagtagaa ggatttaaag aaaaagacaa cggagtggag
      721 gtcctgttga aagataatcc atcccttcag gctgatatgg tagttctggc aatcggagtc
      781 acaccagaca cagtattagc aaaagaagcc ggtctggaac ttggcatcaa tgaaagtatt
      841 gtagtgaatg acagaatgga aacctctgta ccggatattt atgctgcagg tgatgcagtt
      901 caggtaaaac attatgttac gggtaatgat gcgctgattt ctttggcagg accagccaat
      961 aaacagggca gaatcatcgc tgataatatt tgtggcggtg atagtcgtta cctgggcagt
     1021 cagggaagct ccgttataaa agtgtttgat atgacagcag ccactacagg tatcaatgaa
     1081 accaatgcca aaaagtcagg attagaggta gatacagtaa ttctttctcc tatgagtcat
     1141 gccggttact atcctggtgg aaaagtgatg accatgaagg tggtttttga aaaagagagc
     1201 tatcgtttgc ttggcgctca gattatcgga tatgaaggag ttgataaacg tattgatgtg
     1261 ctggcaacag caattcatgc ggggctgaat gcaacccagt tgaaagatct ggatctggca
     1321 tatgcgccac cttattcttc tgccaaggat ccggtaaaca tggctggatt tatgatagac
     1381 aatattgcaa aagggacatt aaagcaatgg catttggaag atatggacaa gatttctaaa
     1441 gataaagatg tggtgttgat agatgtgaga actgtatgtg aattcagcag ggggcatatt
     1501 gatggattca aaaacatacc tgtagatgaa ctgagagaac gaattagtga aattgagaaa
     1561 ggaaagcctg tgtatctgat atgccagagt ggtctgcgca gttatattgc gagccgtatt
     1621 ctggaaggaa acggttatga aacgtataat ttctctggcg gatttcgctt ctatgatgca
     1681 gtggtcaatg accgtgcgct gattgaaagg gcatatgcat gtggtatgga ttattaaaaa
     1741 ataatcacga tatttttata attttcttat agcataaaaa gatcccccat ttattagaat
     1801 gactttcata attctgataa atggaggatt cttctttata tgttacagaa aaacagtttt
     1861 atgacctttg gagtgtttcc agtctttttg gatcaaggat tgtgattttg ccgcgggaga
     1921 gtttgacgag cccctcacct tgaaagtatc gaagcattcg agtgataact tccctgtggg
     1981 aaccaagatg gttggcgata gtttcgtgag tgattttcaa ttcatttgtt tcttcgatag
     2041 aggtctcttc taaaagaaat gaagcaacac gcttatccaa actcttccac atgatttgtt
     2101 caa
//
LOCUS       DAWEJW010000208         2086 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_12833_length_2086_cov_68.877400, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000208 DAWEJW010000000
VERSION     DAWEJW010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12833_length_2086_cov_68.877400"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          478 a          597 c          400 g          611 t
ORIGIN      
        1 atcagcatga gcataacaat ggtcttcatt ccctttacca tgaatttaaa aggtaccttc
       61 gacagacgga tcactaatcc caggaaaagt gccgcaatca cataccctgc ataatttttg
      121 aaaaagaaca gggaaatgat aaataaaacg gtacctccca gctttacccg gggatccatc
      181 ttatgaatca atgattccgt ctgataatat tgtcctaatg taatatctct aagcatattg
      241 ctcctcacca gagtttctgc ccggctcctg tcgggtcaga actcgtatct tctctctgct
      301 catgctgcaa tttgagcatt gcagttcaaa tcattacttc tgattgttct gtttccagac
      361 tcttgcaatt tcttccgcag cctcttcgat cgttgtggca tctacatcca catccgctcc
      421 ccgtgctttc agctcatgca tgatataggt gacctgcggt gctgccagtc ctacttcttc
      481 caattctttg taatgtttga acacttctct cggggtatcg tcatacatga cgcttccctt
      541 gttcatcaca ataatacggt ctacgtattc cgccacatcc tccatactgt gggataccag
      601 cagaatcgta agtcctgtct cggtctgcag ctttttgatc tgctgtaaga tctcatctct
      661 tcccttggga tccagcccgg cagtgggttc atccaggatc agcacttccg gcttcattgc
      721 cagtactccg gcgatcgcca cacgacgctt ctgtccaccg gacagttcaa agggtgactg
      781 atagaaaagc tcgtccggca gtcctacttt tttcagagca tcgtatgcac gaagctccac
      841 ttctttttta tccagtccca gattcttcgg tccgaaacaa acatcgctga atacatcgat
      901 ctcaaacagc tgatgctccg gatactggaa caccagccct accttgctgc ggagcttttt
      961 catatcataa tccttgtcgt agatatcttc tccgttaaaa tagatatgtc cgtcggtagc
     1021 acggatcaat ccgttcatat gctgcatcag cgtggactta ccggagccgg tatgcccaat
     1081 gataccgatg aactgtccat ccggtatctt caggcagaca tcgttcaggg catgaactgc
     1141 cagcggtgta tctgcaccgt atacataatt cacatgatct aatatgatag acattttttc
     1201 gatcctttcc cgggtacttt cattcgctgc catccccgcc actatttcct gtcggttttt
     1261 ccatagccca gtgcattgac cagctcttct cttgtcaata tgccatccgg cagcggaatt
     1321 ccgcgtttct gcaattcata cgataacagc gttacctgcg gcacatccag ccgtagcttt
     1381 ttcagctgct ccacctgtga aaagatttcc cggggggtac cctgcatggc aatttttccc
     1441 tcatccatga caaatacctt gtccgcatga atgatttctt ccatataatg ggtgatcagg
     1501 atcagcgtaa cgccttccac ctgattcaat ccacggactg cacggatgac ttccttacgt
     1561 ccactgggat ccagcatggc agtcggctcg tccaatacaa tacatttggg atgcatggcc
     1621 agtactccgg caatagacac tctctgcttc tgaccaccgg acagcttatt cggggaatac
     1681 tttcgatatt cgtacatacc taccgctcgt aagctctctt ccacacgctg ccagatctcc
     1741 tccgtgggga tccccatgtt ctccgggcca aatcccacat cttcttccac tacctgtcct
     1801 atgatctggt tatccggatt ctgaaacacc atgccggctg tctgacgtac cttccagatg
     1861 ttgtcctcat ccttcgtgtc cataccatcg acccagagtg ttccctctgt cggatttaag
     1921 atggcgttca tatgctttgc cagagtagac ttgccggaac cgttatgtcc gaggatggct
     1981 atgaactgtc cctgctctat atcaagagaa acatcattca ctgctcttgt gatcccttcc
     2041 acatttccct cttcatcccg acgaatatat tcatatacca cattat
//
LOCUS       DAWEJW010000209         2079 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_12885_length_2079_cov_51.099802, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000209 DAWEJW010000000
VERSION     DAWEJW010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12885_length_2079_cov_51.099802"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          586 a          398 c          615 g          480 t
ORIGIN      
        1 ccaggtgaat ccggcgttgg caaagcaggc gcggaaggta ttggatgtga ataaatccga
       61 gcgtcatatc cgcggcggac tggctaccag agagaagtat ctgcaccagc actagatatt
      121 cgatgaggca aatcattcta tacgggaaag cgtcttgaaa taccgggtac caacttatat
      181 aatggataca tagctatatg ggacaaagca gttgggacat aagatacaaa agatatcagg
      241 acctttgcgc ggcagaatca cttatgagga gagaaaaacg aaatggcaac agagtttctg
      301 aatgatgcac ggaaagagat cgagagacgg acagaagact tctacgggga attaaaagca
      361 ttctatcagg gcaacgggga agcggaacag aacctgatgg agcagacaac ccagcctttc
      421 tggcaaagtc tgcgcctgag cagaaaaaga ctgcagcagc gggaactgac ggtggatatg
      481 gagatgcagg agccggcacg actggcggac tatgatggtc cctggaagga tggttatgat
      541 tatagctgcc gccgtaccca gccggtaaaa atgagacgga cttattaccg caagggaaaa
      601 aagattgcgt tcctaaagac accggagatt gcagcggcga gctttttgaa agcggatgtg
      661 cagggagata tggttatctg ccctaactgt ggacatgagg gaaaactgac cagctatatc
      721 gatggctgtg atgcctgcgg ggcaaagttt ctcgtcagtg attttgaaac aaaggtatct
      781 ggattttctc tggaggagga cgcaagacag aaaagtattt ctaactttat gaaagccggt
      841 gctgtggtgg gaattacagc gatttctctt gcggtactgg ccatctgtgc gggcggcatt
      901 atgtttttgc tgttggcgtt gggcaggaac ggatacaatg cggtaaaggc agccgcagcc
      961 atgatgctgg gaattggtct ggctccggta tttttccggt cgttgtttta catgctcatt
     1021 atatttattg taatgttgat cgtgatggag actcgcagaa agccgagaat tcaagacgaa
     1081 tcgaaagtaa aggccgcaat cccggaattc tccacgggta attttcttca gaatctggaa
     1141 taccagctgc gaatgatcca tctggcggat acggcagagc aggtacgctt tttcgcatcc
     1201 tgtgatctgg atacggtggt ggtacggtat cagaatgtgg tggactgttg catttgcggt
     1261 gtgcgttttc tggaggcgga gacacgggga gagtcttaca gaatcagcgt ggaagtgagg
     1321 acccgattga cactggatac aggaaagaag atccggaacc gttatgagga gatccggctg
     1381 gaactggaag gcagacagga tgtggtaacg cagcacagca gggcactccg ggaatacaaa
     1441 tgccccaact gcggaggcag tgtggacatt ttgggcggcg gagtctgtga atactgtaat
     1501 accgcggtgg actaccgaaa ttttggctgg atcatcactt cttatacaaa tctggggcag
     1561 ccagagaatc cttttgcgaa aatactggcg ggagcactgg gaagttatct gctcattttg
     1621 cttttgagtc tggtactcat ggcgcattcc gaggatggaa aagatacttt tgagatctta
     1681 cagagtgtcc ggatgtcaac ggaatatctg aaagcggtgc agcaggatat tatctatccc
     1741 gatgctgtga tttcagattg tacggagaca gacagcgaag agggaacttt tgcttcaata
     1801 aaggtgtact cctgtgggga ccgggatgca gtaaaagaag cctacaaaac tgcactgctg
     1861 gaaaagggat ttctggagat gcagcagtac ccggagggat tttctgtctt tttgccggag
     1921 gatccgtcag aatatgccgt ggattcggaa gaaggaatgt tctatcttgt gatccgcgca
     1981 gaaaatgcac ctgagggaat tacagtaact gcgacactgg tggatgagaa ctgggatccg
     2041 gtacaggaat aaaaatagaa taagaaaatg cgacaagga
//
LOCUS       DAWEJW010000210         2076 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_12912_length_2076_cov_28.406235, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000210 DAWEJW010000000
VERSION     DAWEJW010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12912_length_2076_cov_28.406235"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    326..429
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          554 a          378 c          417 g          623 t
ORIGIN      
        1 ttttattagc tttgatggag aaaactttgt tttctccttt acgtttagcg ttaagtagag
       61 taaattacag tcaaaaccgg cattttctta gcaaaaatca ttgcttttgt aactctatat
      121 aaatttttga atgaaaatac tcattcacag atctgtttat ggtcttgtgg atgagttttt
      181 ttatgtaata gaaaattact tcgtaatgtt cctattacat aaaaaacgct ccgcgaggat
      241 gcgaaaagca gatcagatga tgaacaccga ctattcgatc ctgctgtcga gaaggttgta
      301 gagattggtc tggagtgcct gaggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnna ttcgagaatc atccgttcaa ggtaaaagag gatgaagcaa tggcacgact
      481 catggaaagc attgagagat acggaattat gaatccgctg atcgtaatgc cgacaacgga
      541 aggattctat cgtatcatct ctggtcatcg cagaaaatac tgtgcagaaa aacttggata
      601 tacacaggtg ccggtgatta tccggtacat gaaagaggac gattcgatta tatctatggt
      661 ggattcgaac cttcacaagg aaaagatcag atccagattc ggatattcat caacgatgga
      721 taccagattc tgttttgtgt accagctgtc acaaaaggtg atgatatgat ctttgctgtg
      781 aaattcaggc ataacctgcc ggatcatgga ggctgccagt tccagtttag attctttttt
      841 ttctggatgt ttctgtgata tacttatacg cggtcgtatc agaccggaaa ggaaatatta
      901 tgttacaaaa attatatgtt tttttcagga aagaaaccgt tttgtcaatt gcagtgatcc
      961 tggcgcttct ttctatgttc tgggtcagac cggataaggc ttattttact tatatcgatt
     1021 tcaggactct cgggctcctc ttctgtttga tggcaattgt agctggtttg aaggctgttg
     1081 gtgtttttga tatacttgcc caaaaattgc tgatgggaac atcgggaact gtgggtgtga
     1141 tcaggctgtt ggtactctta tgtttcttcc tgtcaatggt gatcaccaat gatgtggcat
     1201 tgattacttt tgttcctctt gcactgatca ttgtgcataa actgccaaag gaactgggca
     1261 attactggct tcttaaaata gtagccatgc agacaatcgc tgctaatctc ggaagcatgc
     1321 tcacaccaat cggaaatccg cagaaccttt atctatatgc cagagcaggg atgtccgcgg
     1381 cagaattaat aaccctgatg cttccctatt ccgccacagc acttatcctt cttctgatat
     1441 ggatacaggt agccgctgcc aaagcacctc acgtatgcgg ttccgaaaaa gataaaactc
     1501 ttttgggatt ctctgacaga aaagaactta acatggaata cctggccgct tacctgatct
     1561 tatttacaat ctgcctgctc actgttgcac gtatcattcc ataccagatt cctcttgtat
     1621 tggttcttat atatatgctg ctaagaaaca gggaaaatat aagcagggtg gattactccc
     1681 tgcttgccac ctttatcgca ttatttattt ttatcggcaa ccttggaagg attccgcaat
     1741 tcagcagttt tctggaaaga atcatggcag gaagggaaac acttactgct gttcttgcca
     1801 gtcagattat gagcaatgtt cccgctgcgc ttcttttatc cggctttaca gataattacc
     1861 gggctttaat tgtcggaacc aatattggag gtctcggcac tctgattgca tccatggcaa
     1921 gcctgataag tttcaaatat attgcaaaag aaaacaggaa tctgagaggg aaatatttgg
     1981 gaatattcac agcctccaat attatattca tgatattcat gttaatattg tatttttttc
     2041 ttagataaag gttttcccgg aattacaaaa gctata
//
LOCUS       DAWEJW010000211         2065 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_12979_length_2065_cov_61.346766, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000211 DAWEJW010000000
VERSION     DAWEJW010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12979_length_2065_cov_61.346766"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          516 a          546 c          432 g          571 t
ORIGIN      
        1 gggaatgatt gcggtatccg cacagctgga caatgctgcc cggatgatct caaagcaaag
       61 cttcttgtca ttcttatgtc ccagatattt ctttgcaaat ttcttgtcct tctccggcat
      121 attctgtaag aacccaaccg tagtatcgtt atcgtgggtt ccggtataga ccacacagtt
      181 cttgtcataa ttgtgcggca gatattcact gtcatttccg gcatcaaaag caaactgaag
      241 aatcttcatt ccgggatacc cggtcttctt caccaaccgg atcacgctgg gagtcagaaa
      301 tcccagatct tccgcgatca ctttcttcct gcccagtttc ttcttcatga catcgaataa
      361 ttcgtaaccg gggccttttt cccaatggcc gttctccgcc gtcggatctc cgtaaggaac
      421 gaaccagtat tcatcaaagc cgcggaaatg atcaatacga agcacatcat acagctgata
      481 gcaataggcg atccgctgca tccaccacgc ataaccggtc ttcttatgat aactccagtc
      541 gtagagtggg ttgccccata actgtccggt ctcggagaaa caatcgggtg gacatcccgc
      601 cactccgatg ggccggcctt ccttatccag ctggaacagc ttcgggtttg cccaggtatc
      661 ggcactgtca aatgccacat agatgggaat atcccctacg atctcaatgc ctttcttatt
      721 ggcataggct ttcagttctt tccactgttt tgcaaacagg tactgttgga agcaataaaa
      781 tgcgatttct tccgccagct tcttacggta tttgcgaagt gccgctgctt ttctgagacg
      841 gatgtcctcg tcccattcca taaagcagac accgccaaaa gcatttttca ccgccatata
      901 cagagcgtag tcatccagcc agtacgcatt ttccgtaagg aatttctgat agtctcctgt
      961 ctctgtaagg ccctgttccc tggcattgtc aaacgcaatt ttcaggactt taaaacggga
     1021 cagataaatc ttctcatagt ccacataatg ctcattgtct ccgaaatcat agctgtcaca
     1081 gatctctttt gtcagatagc cgtcttcgat cagaagctcc aggtcaatat agtaggggtt
     1141 ccccgcaaag gtggaaaagg actggtacgg agaatcgccg tagccggtag ggcctaacgg
     1201 caggatctgc cagagatggt tgcctgattt ttccaggaag tccacaaaac ggtaactttc
     1261 cttaccgaaa gttccgatac catatgtcga gggaatactg gatatcggca gcagaattcc
     1321 ctgttttctt acatttttct tcatagcttt tgtattcctt tatatttctg tgctttcgta
     1381 cattacttat ccgatgctct ctgattaaaa aaataactat tcagtccttg tcatgagctc
     1441 tgaatagtta tttcgagtgg aagcaagggg gctcgaaccc ctgacctttc gcgtgtgagg
     1501 cgaacgctca tcccggctga gctatgcttc cataattacg caatgcggat aacaggactt
     1561 gaacctgcac gtctgttgac aatagaacct aaatctatcg cgtctgccac ttccgccata
     1621 tccgcttact aactgccata tccgacgatt agcttaaaac cttgacaaac gggaatgctc
     1681 tctgccaaag gaccttgaat aattatactc ttccttcctt ttgcttgtca agcatccccg
     1741 ccgcatctgt ttctatttta ttctgtaatc tctcttgtcg ttaccggttc tccaatgtgg
     1801 cagatgatct ttttatatat ccttacgagg aaataggtac ttaagagcag gctggccagt
     1861 tccgcgatgg gaaaggccca ccagatcaat gtcacttcac cgcttaagga caaaagcttt
     1921 gccaccggaa gcagcacgca catctgtctg gccacggaca cgatcatact gtagacgccg
     1981 ttacccagtg cctggaatac gcttcccacc acgatacaga agccagcaaa cacaaagctt
     2041 agggaaatgg tctccagcgc cggaa
//
LOCUS       DAWEJW010000212         2051 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_13086_length_2051_cov_56.703908, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000212 DAWEJW010000000
VERSION     DAWEJW010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13086_length_2051_cov_56.703908"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          558 a          374 c          486 g          633 t
ORIGIN      
        1 tatattgata tttatttcgg caatctttgt aaacaacatc gtgttgtcgc agttcttggg
       61 tatctgtccg ttcctaggcg tatctaagaa agtagagact gcgatgggta tgagcgctgc
      121 ggttgctttc gtgttgacca tcgctaccat cgttaccttc ctgattcaga agtttgtatt
      181 ggatgttttc gggctgggat atttgcagac gattacgttc attctggtca ttgccggatt
      241 ggtgcagatg gtggaaatca ttctgaaaaa agtatcgcct gctctgtatc aggctttggg
      301 cgtgttcttg cctttgatta caaccaactg ttgtattctt ggtgtggcta tcctggtgat
      361 tcagaaagac tacgacctgc tgacaggtgt tgtgtacgcg ttttctacgg ccatcggttt
      421 cggtctggca ctggtgcttt ttgccggatt gcgcgaacag atgagcctcg tgaaagtacc
      481 taaaggaatg caaggcactc cgattgcctt gattaccgcc ggtttgcttg ctatggcatt
      541 catgggattc tccggcgtgg tgtaaggatt tacagttgat aattaaatag gggattggaa
      601 gctctgctgt atggcgtcat acagctttga gtttccaatt cttttttata cctgattgcc
      661 acccaatcgt acctaaatta cttttcagca aataaaattg tggcaaatta ttgctattcc
      721 attttatttc catacatttg taactcaaac aatgataagc cgagaaacta accattttta
      781 taaagtatat gaaagaaaga attttagtaa caggcgggac aggatacata gggtctcata
      841 ctgttgtaga actacaaaac agtggatatg aagtaatcat catcgataat ttatcaaatt
      901 caagtgctga tgttgttgac aatatagaga aagtttccgg tattcgccca gtttttgaaa
      961 agctggattg tctggacttt gctggtcttg atgcagtatt tacaaaatat aaaggaatta
     1021 aggcgatcat tcactttgca gcgagcaaag cagtaggaga atcggtacag aaaccgttgc
     1081 tttactaccg taataacctg gtttctttga ttaaccttct cgaactgatg ccaaaacatg
     1141 gagtagaagg tattgtgttc tcttcttcct gtacagtata cggccagccg gatgaattgc
     1201 cggtaacaga aaaggctccg attaagaaag cggaatcgcc atacggaaat acgaaacaga
     1261 ttaatgaaga gattattcgt gatacggtag cttcaggtgc tccgatcaat gcgattatgc
     1321 tgcgctattt caatccgatt ggtgcgcatc ctacggcatt gctgggtgaa ctgcctaatg
     1381 gggttcctca aaacctgatt ccatatctga cccaaaccgc catcggcatc cgtgagaaac
     1441 taagtgtgtt tggtgatgat tatgatacac cggacggttc ttgtatccgt gactttatca
     1501 atgttgttga cttggctaaa gcccacgtga tcgctatccg tcgtatcttg gaaaagacac
     1561 aaaaagaaaa agtggaagtg ttcaatatcg gtacaggacg tggcgtttct gtattggaat
     1621 taataaatgg ctttgaaaga gccaccggcg taaaattgaa ttatcagatt gtcggacgtc
     1681 gtgcaggcga cattgaaaaa gtttgggcga atccggattt cgcaaaccag gaactgggtt
     1741 ggaaggctgt agaaactttg gaagatactt tacgttctgc atggaactgg cagctgaaac
     1801 tccgtgaaag agggattcaa taaaagagat ttgttttaaa ataaacataa aagattaaac
     1861 aaatcgtgca tgcgatttgt ttatctgatg ctaatcgacc tgaagctgtt tatgagtatg
     1921 cgaatatacg taaatagtac ttatgcctcc ccggcataga caggaaagat tgcatagtag
     1981 ttttgtcgtg ttttattttg tgtttgtgtt gtgacggtac tacgacgtga gtcgtggtac
     2041 cgttttttgt t
//
LOCUS       DAWEJW010000213         1949 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_13933_length_1949_cov_55.098733, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000213 DAWEJW010000000
VERSION     DAWEJW010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13933_length_1949_cov_55.098733"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          576 a          409 c          580 g          384 t
ORIGIN      
        1 tcaaggtgga tgagtatcag gcaatctatg atgagaacct gaaagcactg acggacaaga
       61 tggacgaatg gggtatcagt tggtcgcagg tcacaggcgg tggaggttcg gtacagatcg
      121 gtggcggagc cggcgctaat atgcagagcg gcggaacttc caacgccaac attttagctt
      181 ctctttacag tattctggaa caaaatctga aagatttgga ggaagcagag gataaatatg
      241 aggatgcatt aactaatgct gcttttgaat tacaaacttt gcagttaaag ctttccagtc
      301 tccaacaggc agtcacggag gcgaaggaag attatgagat ccagctggcg caggcaaagc
      361 tgacctatga gacttcccag tccggcgcag aacgggcaga gagtgattat aatactacgg
      421 tggaaaaagc taagtccgat ctggcagctc tgaaaagtac ttatgaagat gccaaagaga
      481 atctggaact gtttgaaagc agtgtgggag acggatattt ctatgcctcg gaggacggaa
      541 cgatcctgcg gaccatggtg cgggcggagc aggctctgac ttccgatgca gtggtattcg
      601 tttacagcaa tccgaaggag ctgacggtca ccgtatcggt agatcagtcg gatattacaa
      661 aactgactgt aggcgacagt gcttatgtgc agtcctccgc aggcagcgga tataccggtg
      721 tgatcacagc cattgatccg gtatcctcgt ctagcagccg taccagtgtc acttacagcg
      781 tgacggtaca gatcaatgta gaagatgagg aggacagttt aagtgccaac gaatccgtca
      841 ccgtagtctt cggcatgacg gaggaagaga tcgagcagtt acagcagaga cagagtatgc
      901 agggcagacc tgacgatgtg tccgacggcg atgcgatgcc gcagggcggt cccggaggcg
      961 gcgaaaagct ggaaaatggt gagatgccgg aaggagcccc gggtaacgga gaagtgccgg
     1021 aaaacggtgg catgccggaa ggagtcccgg gcagcggaga agtgccggaa aacggtggca
     1081 tgccgaaagg cggctccgac aacggggaat ctacggaaaa tgcaaatgaa cagcagggag
     1141 gacagacaca gtcatgaaat taaaaagccc acattttgat aaactgaaat ccctcacgaa
     1201 aaagcagaaa aaactgatcc tggcagggat cgcattactt ctgctgatcc tggcagcctg
     1261 ctataccgtg tttattgcac cgttacttca gaaagaacag tggatctaca aagaagagac
     1321 ggtggagcgt ggtacattaa aggtaggtgt tactgagagc ggctctctgg aatacaacac
     1381 aaaatccata gattacgatc tggatctgga cgtcagtgat gatgacgagg atgacagtga
     1441 tgatgacgat gataccgtac aaaaatatct gaagatagag gagatctacg ccgccagcgg
     1501 gcagcgtgtc atggaaggcg aggaacttct gaaatttacg gaagacagcg tggaggcggt
     1561 tcgggcattg ttgcagaatg cggtagtgga ggcacagtcc gactacgcgg aggcagagag
     1621 tacttatgaa ttgtccctgc tggaagccca gacggattat gatacccaga agatcagtgc
     1681 ttcctatgcg gcatccatcc gcaatacttc cgggaccagc gtgacgaaca atgtggcgtc
     1741 tctgcaggta cagctggagc agaagcaggc gaataccgca tctttgcagg agaaattgac
     1801 gcaagcacgg gaagattatc aggatgcgtt ggagacctac gaagcggcaa aggaaggcta
     1861 tgaaggtgcc ggaaccgata atacagtgaa tttcatgacg atccagaacg gttatctcag
     1921 cgcaagaaca aaatatcaac aggcaaaat
//
LOCUS       DAWEJW010000214         1921 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_14184_length_1921_cov_87.000536, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000214 DAWEJW010000000
VERSION     DAWEJW010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14184_length_1921_cov_87.000536"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          529 a          395 c          519 g          478 t
ORIGIN      
        1 ctgttcaacc ggaatgcatc ttccggagga ctggatattg tggcgaagat catgaataaa
       61 tatctccata tggaactggg taaggctatg tctctctccg gtatgtgtgt ggcactgtcc
      121 gcagcactgg tatatgataa gaaaacggtg gtgctgagta tcctgggtac ctatttcaac
      181 ggaatcattc tggatcattt tatttttgac aataatatca agcgacgtgt ctgcattatt
      241 acgaagaaag aggaagaact gcgccagttt attacaagag atctgcacag cggtgccacg
      301 atctatgaag ccatcggtgc ctatcatttt gaaaaacaca acgagatcat caccattgtg
      361 gacaaaagtg agtaccagaa attaatgaaa ttcatcaatg aactggatcc caaggcgttt
      421 attaccgtat ataatgtatc gagtatgaag tatcagccga agaccggggc cgtttgaccc
      481 tgactttgat gcctacggat tgttccgtgc tacgcactct cacaatccgc ccctctattc
      541 gcatatcgtg ggattatcat cccactttta tgctcatatc ggggcgggtc ataaggtctt
      601 tcacccactt atgagcaagc tcatgtgggc ttagaccttt tgaccctggc atcttaataa
      661 tatggttatg gagaaatgcg aaatgaaaaa ggtacggaat atcgcagtga tcgttttatc
      721 tgtcatatgt gttggaattc tggttttttt gttgtggaat ttaagaagta cagaggagtc
      781 tgcacaggaa ataataccgg aagttcagcc ggagcctatg tcggaacaga gtgtatcgga
      841 ttctgatgtg agcggcaacg gtgcggagaa cagtgcagat cctacggaat tgccggagac
      901 agaagaaagc gaaccggtgg aaacggaaga tcctgtagag cagagggcag aagaactcct
      961 ggcaggaatg accttagagg aaaaagtcgg acagatgttc atcgcccggt gcccggagac
     1021 ggatgcagca tccaaagtga cgcagtatca tctcggcgga tatatcttat ttgcccggga
     1081 ttttaccggg aaaacaaaag aggaagtaac agcagccatt caaagctatc agaatgctgc
     1141 aaaagtgccc ctgctgattg gtgtggatga ggaaggcggt acggtaaacc gtgtcagcaa
     1201 aaatgcgaac ttgcgggaaa cgccttttgc ctctcctcag gaactgtatg cgcagggcgg
     1261 atgggatctt attcggagcg acacacagga aaaatgtcaa ttactgcaga gcctgggaat
     1321 taatctgaat tttgctccgg tatgcgatgt atcacaggat ccgcaggatt ttatttatgc
     1381 ccgcagtttc gggcaggatg cggaacagac gacggagtat gtgaggaccg tcgtgcagac
     1441 catgtcgcag gaacagatgg gaagtgtatt aaagcatttc ccgggatatg gcaataactc
     1501 ggacacgcac acagggatcg cttatgatga acgtcccata gagaggtttg taaattctga
     1561 ttttctgccg tttcaggcag gcatcgacgc aggggcggat atggtgctgg tagcccacaa
     1621 tgttgtctcc tgcatggatg atcaggtacc ggcatctatt tctctaccgg tacataatat
     1681 tttgcgtaat gaattagggt ttgacggcgt gatcattacc gatgatctgg tcatggaagg
     1741 agtacggcag ttcgccgggg atgcggagat tgccgtccgg gcggtacagg caggaaacga
     1801 tatgctgtgc tgcaccgatt ttgaagtaca ggtacccgca gtcatcaagg cggtcgaaac
     1861 cggtgagatt accgaggaac gcctcaacga atcggtcctg cgtattttga aaatgaaaat
     1921 a
//
LOCUS       DAWEJW010000215         1910 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_14284_length_1910_cov_60.314286, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000215 DAWEJW010000000
VERSION     DAWEJW010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14284_length_1910_cov_60.314286"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          495 a          522 c          357 g          536 t
ORIGIN      
        1 cccagcagtt cttccatgtt ggtatcgata cataccggcc ggatcttgcc actctgtatg
       61 atatccctca cggacagctt ttccagactc ttcatggtaa tagtatccag ctcactattg
      121 tggttcaaga tattccacag aaaatctccc tcactgacta cgcccacata ccggtcctcg
      181 gcatcgatca ccggtaccgc tgtgtaaccg tagcgtttca tcttctccag cccctgtctg
      241 atagtcatat cgtcccgaag aaaagtaacc tcctccttcg gggacaaaaa agatgcaata
      301 ttcacaatgt tcctcctgct tgttcttttg tgtccgttaa ttttgatcct tagggtcgac
      361 ctcttcgaag gtactgtcca aggcatcctg ctcctgtttg aatctccgga gcttgcgcgc
      421 aaaataaatc gtagaaaagc aatcgccggc accactgaga atcagtacca ctcccagaac
      481 acggaacaac agaatcgctg ctttgaacgg attgtagatc agtaccactc cgagaatgat
      541 gttgattgcc gccacaataa gaagtcccag ccagctgccg taacccagtc ttttcaggtc
      601 aatggcatcc tgcagtttgc tgcatccgct acacagaacc agtactccca gaataaaggg
      661 aatcagggag atgacgatct ctaccttgta caggactact gctcccaaca cgattccgag
      721 aataccgaat acaaactcat tgtgataata gttctctctg gcatccctga gcagatagca
      781 gataatagat accagtcctg ccacgatcaa tacgataccg atcagatacc ccagtgtctt
      841 ctgcatggtc tccgggatca ccagtgccac gattcctaag agaatataca gcacaccggt
      901 aagaattgct tcccatttca attctttcag cattttcata tgtaaccctt cctttcctgt
      961 aaaacaatgt taaactcagg gaaaacgaaa aaggggctct ctgcaaacct gcggaaaacc
     1021 ccctttatgt tcaatttact ttctgccgca gcacttcttg tacttcttgc cgcttccgca
     1081 gggacaggga tcattgggat aaaccttctg ctccttcaca atggtagtgg aagacttctg
     1141 ctctttgtag agttccttac gggtctcttc gtcaaagata tcgttccact cttccagatt
     1201 gtacagccag tcggcttctg ctgctaccat gttcttgtac agaagagcct tatcgaagcc
     1261 taagtttacc tgagtatcct cttccagatc atcaatgggg ttctgctcta ccaggctgtc
     1321 gttgatgcca tccaggaatc cggtcatggt catcagagat actttgaact tgtcagccag
     1381 ttccttcacg gtacccttta ctacttcatc gggattcttc agaagctgtg cgtagatttc
     1441 cttctcctcc tggaaataag ctgcccagag ctgctgcagc ttctctttgc cggttgcctc
     1501 attatatgct actgatctcc actgttccat taaattcata attgtaatac catccttaca
     1561 taataattaa atatttaata atacgatttc tccgcccttc gggctctcga aatcgtcaca
     1621 tccattcgca tttcggactg gcgtcctcca tgctcatgtc tgtttatccg ctcaataggt
     1681 gaactgcctt gcctgcaagc agtcaggcag ttcacctatt ggcagaatta ttaatcatag
     1741 tatataacaa ctcgtcctca aaaacaacaa aaaaatatca atcggggatg tataaaccca
     1801 caaatccggg acagactact actatcatcg gatcccctcc tttgatctta cgaaagatac
     1861 gataatactt aatcctcccc ttttgaaaag aggcacccgt gggtgcctct
//
LOCUS       DAWEJW010000216         1896 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_14410_length_1896_cov_81.263444, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000216 DAWEJW010000000
VERSION     DAWEJW010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14410_length_1896_cov_81.263444"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          443 a          504 c          407 g          542 t
ORIGIN      
        1 cgatttttgt gtaacgaaaa aatcagcggg tatatagcat cccaaaagct ctctcctgcg
       61 agagagcttg gtctgcacct gtaaagtcaa ggttgttttg gtgtaaatag cataaaaacg
      121 acaaattcca tacaaaaaat tccccaaatc agaaattttt gtacggaatt tacttatttt
      181 gcttatatag cgaaaatgga gacttttcaa acggtaaaat tccacatttt tgtatttttt
      241 tcgtccaaaa cggcgattta aatacaaaca agatttccac tgactgtccc gatgcaacgc
      301 tcacaaagag aatttctatc actctgcgaa cagttccttc ttccgttcaa tcatctcatc
      361 aatcttctca atatacagcc ctacatcctt gacattatcg caccgttcga tccgtccatc
      421 ggggaagact ctcttggtga tgcttccggc tcccaaagcc acaatggtct gtttctcctc
      481 catgatgagg atgttataca ggccgtattt tccggggcgg gcgtagccgg tgttctcgaa
      541 attaccggac atattcttct gtcggtagag ataatacggc ttcattccaa gcttttcagc
      601 tcccgccgcg atcttcatgg tttcgtcggt gttgtgaagc atggagacgc cgttctcctg
      661 gatccactgg ttcaggcggg atgcacgctt gattgccagg gaatgaacgg tcaggctgtc
      721 cggggccagc tccacgatct tttcgatggt gcgctgtaca tccgcctcca gttctcccgg
      781 caatcccagg atcagatcca tattgatatt atcaaatccg acctctctcg ccagctgata
      841 tgccgtaagt acctgttcca cgctggcctt tctgccgatg atatccagtg tctcctgctt
      901 catagtctgg ggattcacag agatgcggct gacctgatga cggtacagta cctccagctt
      961 ttctctggtg atgctgtcgg cacgaccggc ttccacggtg aattccttca cggtagagaa
     1021 atcaaacttg gactttaatg ccgtgatcag acggtccagc tgctccggtt ccagtgtggt
     1081 gggcgtacca ccgccgatat agacggtatc cagtaccttg tcctgaaaat tctccgccac
     1141 ataatccatc tctttgatca cagcgtccac ataggcgtcc acctgcctgc ggaatgcagc
     1201 gatcggataa gaggtaaagg aacaatagag acaggttgta gggcagaacg gaatcccaat
     1261 gtacaggctg taaccacctt cgtaatgaat ctcttttaac agatcccgct ctctcttcgc
     1321 aatatcgatt cccaaaagtg ccttctcttc gctgacgtaa tggctctgct ccatgaattc
     1381 cagaatctct gcatcgcttc tgccctcatc cagcataccg taggcgatct tcgtgggacg
     1441 gatgccggta aggtttcccc atgggagttt ctgcccggtc accttgctta aggtgcggta
     1501 caggaaacgt ttcagaccgt tcttataccc atctgcaagg ttttctgtct ctgcatccca
     1561 cagaaaatcc tcttctccca cacggatctt cgctccggtc tcggccagtt cgatttccag
     1621 actgacctgc ggttccagct ctgccagctt ctccggtttt gtctccgggg tgatgaccgt
     1681 gacctggtcc tccgcataaa aagccttcac cagagaatgt acatcatatt catagttagg
     1741 atgggataat ttcactactt tcattgtatt ttcctcattt acataaatac aggcacagag
     1801 ataactcttt ctgtgcccga atactttttg atttaatcaa cgcaaaacgg attatatttc
     1861 ttctcatgtc cgatggtggt gctgtcgccg tgtccc
//
LOCUS       DAWEJW010000217         1884 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_14532_length_1884_cov_59.029524, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000217 DAWEJW010000000
VERSION     DAWEJW010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14532_length_1884_cov_59.029524"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          442 a          513 c          406 g          523 t
ORIGIN      
        1 ttttgtattt ttttcgcaga aattggcgtt ttaaatacaa acagaactcc cggaggactt
       61 tctgccgctt caggagttct gcatttcata aaaaccgtta taaatgcact ttttatgaaa
      121 aaagaactgt ataaaagaag ttttttcata aattcggcaa agaaaacatt tttatgaaac
      181 gaatagcccc ggcaaggtat aaggaaaagg tggccacctt tcttataccc tgccaagggc
      241 tatcattttt taactagctt tccacttttg tcactatctt attttcttcc cagccggatc
      301 acattccagg acgctttcct cagcacggtc tccaactggc cgccttctgc tttggcatcg
      361 tcggcctgac gggggtgtac tttctctcct gctgcggagt tcacggtctg taagtcatcg
      421 gataccagct cggtcttgcc gatcagttca tagccttcga aactgcgcag gtccatggtc
      481 aggggcaggt cttcttccag attacggttc acggcaaata cggtcacttc ctccgcttcc
      541 tcattccata ccgctaccgc ttccacatct gtgatgcttc cgtggccgct ggtctcatgc
      601 ttcgggctgt cgatagccag ctgtaataca gttcctctgc catagcagga tgcatgatag
      661 aaaggataaa aaatggtctg tctccaggca atcccgttct cctccgtcat gatcggtgca
      721 atgacattca ccagctgtgc cagacaggcg atctttacac ggtccgcatg ctttaacagc
      781 gtgatcatca tcagacctac catcagggca tcttcaaagg tataatgatc ctccagcaga
      841 tgaggggctt tctgccaggg atgattctct gtggtgtcgg catcctcctt gctggcgtgg
      901 aaccatacat tccattcgtc gaagcttaag ttgatctgct tcttgctgcg cttcttcgct
      961 ttgacataat cgcagacaga cactaccgta cggatgaaat catccatgtc atcggatttt
     1021 gccagataat cattgctgtc catagctgca ttgccgtaat aggtatgcag ggacagataa
     1081 tctacataat cataattgtg ttccagtgtg gtagcttccc actgagggaa agtaggcatc
     1141 tcccgataag aacttccaca ggataccagc tcgatggaag gatcgatcag tttcatggcc
     1201 ttgcccgctt cctgcgagat ccgtccgtat tcgtccatag tcttgctgcc cagctgccag
     1261 gagccatcca tctcattacc caggcaccac agtttgatat tatagggatt ttccacaccg
     1321 tgggatctgc gaaggtcgct atatttggag ccgccgggat ggttgcaata ttccagcaga
     1381 ttgcaggcat ctgccacacc tctggtaccc agattcactg ccatcatgat ctgtgcattc
     1441 acctgcttcg tccaatggta gaattcctcc aatccgaagg tattcggctc cagagatctc
     1501 cacgccagat ccagtctggc cggacgctta tccacagggc ctacactgtc ctcccagaaa
     1561 aagttggata cgaagttacc accgggataa cggatcaggg gaacctgcag ttctctgacc
     1621 agttgtacca cgtcctgtct gtaaccgtat ttgtccgccg tgggatgtcc cggctggtag
     1681 ataccgccgt acaccgcccg gcccagatgc tcaataaagg aaccgaacag acggtcatcc
     1741 accttcgcca ccttgtatgc cttatctaat gtaatcttaa cctgtttcat gccccaatct
     1801 ctcccaagtt ttttctataa atgatttctt cgttcttcga actcccgaaa ttacaacaaa
     1861 catgcgtaat ccggtctgat cgcc
//
LOCUS       DAWEJW010000218         1798 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_15318_length_1798_cov_42.784280, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000218 DAWEJW010000000
VERSION     DAWEJW010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15318_length_1798_cov_42.784280"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          483 a          364 c          444 g          507 t
ORIGIN      
        1 tcttataccc tgccaagggc tacggggatt tgatagcatt tctttactaa ctctctattt
       61 acatctctgt gctgatagta ccacttgtaa agtcttgtcg gaatcggtat aatatagata
      121 gattgggagg taaaaatatg ctgcttacat tagtaccgct ttttgatgaa aatatggccg
      181 tttgtgccta ctcctttttt acccagaggg agaatttcct gttgaatcct ctgttgcttg
      241 gaaccgcaca gttcgacggg gcatcccaga tcacgggtct ggaactgatc cagaaaatgg
      301 gtattgatac cttgtctcag ggaaaagaaa tcttcgtacc gatcagcaat atctcgattt
      361 ttgcggatat tccggagcag tgcgatgcac ctcatgaaaa aatcgtactt ttgatcgata
      421 ataccattcc gccgattgaa atgtatgtca atcgtctgaa ggaattgaaa cagcaaggct
      481 ataaacttgc cattcgtaag cttgcggtca gtgattttga aaattacaga gaagtcctga
      541 agctgatgga ttatgtcctt ctgaataacc gtaaaatagc cattgataaa gccaagattt
      601 atttcggaaa attatttccg aatatcaatc tgtgtgccgg taatattgat accatggagg
      661 acttcgagag actgaaggag accggcggat atcgtttcta tgagggtaag ttctatcgag
      721 ttcccattac gaagggacag acggatgtgg cacccttgaa gggtaactat attgacctgc
      781 tgaatattgt gaacagcccg gattttgaac tcacgacagc ggcggatatc atcagccgtg
      841 ataccgcact tacgatcgat ctgctgaaaa tggttcagcc actggctgta aattcggaga
      901 ttacatctat ccgtcatgcg gctgccatgc tgggacagcg ggaactgaaa aagtggatca
      961 acacggcagt agccaatgct ttgtatgcgg ataagccgaa tgaggtcacc agattatctc
     1021 tgcttcgtgc gaaatttgcc gagaatctgg cggaagcgtt tggcctgaag gcgcagaagg
     1081 atgaattgtt cctgatggga ttgttctctg tgctggatgt gatccttgag aaacctatgg
     1141 cagaggcttt gaaggtggtc catgtggctg gcgagatcag caatgcactg atctaccgca
     1201 tcggcgtact ggcaccggtg tacgatttta tgctgcagta tgagacagcc aactgggcgg
     1261 aggtatcccg cctgatgcta ttaaagaata ttgatatgaa tacagtatat gaagcctata
     1321 catcggcgtt gaagtggtat cgcaccgtac gttgattgta ttaaaaaatg tacttgtatt
     1381 ttgtatacat ttatgttatt ttcttcaaga acttgtaatt atatcctcca taaaagggga
     1441 ggaatagtag caaaggcgca aacagcgcgg aaaggaaatt tcatatgaaa aagaacaaga
     1501 cacttgagat ggtacaattg gccgtactca tcgctattat gctgattctg gcgtttacac
     1561 cgcttggata tctgcgcatc ggaccgttag caatctctct gatgaccatt cccgtagtaa
     1621 tcggtgcaat gatcctggga cccgcaggcg gagccgtact gggactggta ttcggactga
     1681 caagctttta ccagtgcttt gcaggagatc cttttggagc agcgttggta gcaatgaatc
     1741 cgtttttcac tttcctggta tgcatcccta ccagaactct gatgggatgg ctgtccgg
//
LOCUS       DAWEJW010000219         1791 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_15381_length_1791_cov_50.101382, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000219 DAWEJW010000000
VERSION     DAWEJW010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15381_length_1791_cov_50.101382"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          506 a          366 c          489 g          430 t
ORIGIN      
        1 tgatcggaca gttctccgag aacaaataca acaaggtaaa ctgcggtatc atggaccagt
       61 ttgccattgc catgggcaaa aaggataatg ctatttttct ggacaccgca actctggaat
      121 atgagtacgc accgatccat ctggaaaatg cgaagatcgt tatcgcatgc agcaataaaa
      181 agagaggcct gggagattcc aagtacaatg agcgtcgcag cgagtgcgag actgcactgg
      241 cagaactgca gcaggtagtg aagatcaaga ctctgggtga tctggacgag gagactttcg
      301 agaagttcaa agctattatc aagagcgatg tccgcagaaa gcgcgcaaag cacgctgtat
      361 atgagaatca gagaacgatc cgtgcggtag aagcgctgaa gaacaacgat gtgaagctgt
      421 ttggtgagct gatgaatgct tcccatgtat ctttgcggga tgattatgag gtaacaggta
      481 tcgagctgga tactctggta gaagaagcat ggaaggtaga cggtgttatt ggttcccgta
      541 tgacaggtgc cggtttcggc ggatgtaccg taagcattgt aaaggatgag gctattgaca
      601 cctttatcga tcaggtaggc aaggcatata aggagaagat cggttatgca gctgatttct
      661 atgtagtaga gatcggtgac ggcccccaga ccctgtaaga aattaagatc cgacgagaat
      721 agagagtgcg atgccggaga aatccggtat tgcagaatgg agaaaaaatg gatattcagg
      781 tattaattcg taaattggta tcctacggag tacagacagg actggttccg gaggaagatg
      841 tgatctacac gaccaaccgt ctgttggagc tgtttgaact ggatgaactg gaagagcgcg
      901 acgatgtgac catgcgtgag gatgaactgg aagaggttct gaagggcatg ctggattacg
      961 cttatgaaaa aggcattctc aaggagaaca gtgtggtata ccgtgacctt ttcgatacga
     1021 agatcatggg actgttgatg cccagaccca gtgaagtaat ccgtcatttc cacgaactgt
     1081 acgagcaggt atctcccgag gcagctaccg actattatta caaattaagc agagattccg
     1141 actatatccg ccgttatcgt atctgcaagg atatgaaatg ggtagcaccc accaaatacg
     1201 gcgatctgga cattaccatc aacctgtcca agcctgagaa ggatcccaag gctattgctg
     1261 cagcgaagtt ggcaaaacag gccggttatc cgaaatgcct gctgtgccgt gaaaatgaag
     1321 gttatgcagg ccgtgtgaat catcccgccc gccagaacca ccgcatcatt ccggtgacca
     1381 tcaatggcag ccagtgggga ttccagtatt ctccgtatgt atattacaat gagcattgca
     1441 tcgtatttaa ttctcagcat gtacctatga ggattgagca tgcgaccttc tgcaagctgt
     1501 ttgattttgt aaagcagttc cctcattatt ttgtaggttc caatgcggac ctgcccatcg
     1561 taggcggttc tattttaagt cacgatcatt tccagggtgg tcattatgag ttcgccatgg
     1621 ccaaggcgcc tgtagagaaa aacttctctg tagcaggtta tgaagatgtg gatgccggta
     1681 tcgtggcatg gcctatgtct gtgatccgcc tcagcggtat ggataccgac cgcattattg
     1741 cactggcaga tgtgatcctg aacaagtgga gagaatatac ggatgaagag g
//
LOCUS       DAWEJW010000220         1730 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_16016_length_1730_cov_51.846567, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000220 DAWEJW010000000
VERSION     DAWEJW010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16016_length_1730_cov_51.846567"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          518 a          363 c          495 g          354 t
ORIGIN      
        1 tcaaggtgga tgagtatcag gcaatctatg atgagaacct gaaagcactg acggataaac
       61 tggatgagtg gggaatcagc tggtcccaga tcaccggcgg gtcagccgga tctgtgcaag
      121 cgggtggaac cgcaggcaat gtgctgggca gcagtggaca ggacagcaat ttctcgaatg
      181 tgaaaactct ggcatcccta tacagcattt tggaacagaa cctgaaggat ctggaagaag
      241 cacaggaaaa atatgaggat gcagtcacca atgcttcttt taacttacag acattgcagt
      301 tgaagcttcc cagccttcag caggtagtca cggaggcgaa ggaagattat gagatccagc
      361 tggcgcaggc aaagctgacc tatgagactt cccagtccgg cgcagaacgg gcagagagtg
      421 attataatac tacggtggaa aaagctaagt ccgatctggc agctctgaaa agtacttatg
      481 aagatgccaa agagaatctg gaactgtttg aaagcagtgt gggagacgga tatttctatg
      541 cctcggagga cggaacgatc ctgcggacca tggtgcgggc ggagcaggct ctgacttccg
      601 atgcagtggt attcgtttac agcaatccga aggagctgac ggtcaccgta tcggtagatc
      661 agtcggatat tacaaaactg actgtaggcg acagtgctta tgtgcagtcc tccgcaggca
      721 gcggatatac cggtgtgatc acagccattg atccggtatc ctcgtctagc agccgtacca
      781 gtgtcactta cagcgtgacg gtacagatca atgtagaaga tgaggaggac agtttaagtg
      841 ccaacgaatc cgtcaccgta gtcttcggca tgacggagga agagatcgag cagttacagc
      901 agagacagag tatgcaggga ggacagacac agtcatgaaa ttgaaaagac cacattttga
      961 aaaactgaaa tccctcacaa agaaacagaa gaagctgatc ctggcgggga tcgcattact
     1021 tctgctgatc ctggcagcct gctataccgt gtttattgca tcgttacttc agaaagaaca
     1081 gtggatctac aaagaagaga cggtggagcg tggtacatta aaggtaggtg ttactgagag
     1141 cggctctctg gaatacaaca caaaatccat agattacgat ctggatctgg acgtcagtga
     1201 tgatgacgag gatgacagtg atgatgacga tgataccgta caaaaatatc tgaagataga
     1261 ggagatctac gccgccagcg ggcagcgtgt catggaaggc gaggaacttc tgaaatttac
     1321 ggaagacagc gtggaggcgg ttcgggcatt gttgcagaat gcggtagtgg aggcacaggc
     1381 agactatgcg gaggcagaga gtacttatga attgtccctg ctggaagccc agaccgacta
     1441 cgatacccag aagatcagtg cttcctatgc ggcatccatc cgcaatactt ccggtacgag
     1501 cgtggcgaac aatgtggcat ctctgcaggt acaactggag cagaagcagg cgaataccgc
     1561 atctttgcag gagaaactga cgcaagcaca ggaagattat caggatgcgt tggagaccta
     1621 cgaagcggca aaggaaggtt atgaaggcgc cggaatcgat aatacagtga attttatgac
     1681 gatccagaac ggttatctca gcgcaagaac aaaatatcaa caggcaaaat
//
LOCUS       DAWEJW010000221         1676 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_16622_length_1676_cov_43.359655, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000221 DAWEJW010000000
VERSION     DAWEJW010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16622_length_1676_cov_43.359655"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          391 a          446 c          376 g          463 t
ORIGIN      
        1 gggctttgac tcagcatatt gaaacactgt cacagtcata tattatatgg caccaggcaa
       61 ttagtgtcaa gataatttta aaacttttta cagttcagcc gtaaaacgta acaatgcggc
      121 tttgcagatg acgcatgctg aactgtcaca gaaactcgta tttcatcagc gtcagtccat
      181 gggccggtgc ggtaggtccc gctgcactgc ggtcttttgc ctcgaggatc gtgaaaatat
      241 ccatgggatc ccgcttgccc tgtccgatat ctaacagggt tccggcaatg atcctcacca
      301 tattatataa gaagccatta ccacagaccc ggatcaccag atcattttct ccctgctctt
      361 ccacttccac ggaatagatg gtgcgtacgc tggattccac ctgggcaccg gtctggcaga
      421 agcttttgaa atcatgttct ccctccagat aagccgcagc ttcctgcatc cggttcacat
      481 ccagttcata ataagtaaac agcgaataca gacgtttcac cggcatgggg aactgtgccc
      541 ggtagatccg gtattcatag gtcttgcggc tgtcgcatct gcggggatgc cagtcatccg
      601 gcacctgttc ggacttttgt atccggatat cctccggaag tctctggttc agcgcataag
      661 aaattttctc cgcaggcatc cggctggtcg tgtcaaacac agcgatattc cccagtgcat
      721 gcacaccgga atctgtccgg ctggcaccga tgacctcaac aggctcccgc agcagatcgg
      781 tcagacaccg gttcagttcg ctttcgatgg tgatcccgtt attctggatc tgccagccgt
      841 gataattcgt accgtcgtag gccacggtca ggcggacacg ctttttttct ttttccggct
      901 gctttgacag ctgttctatt atcatttccg attccactgt atttcacccg ctttctctcc
      961 tgccacctgt cttaggttcc ccggcattct ttcctgtatt tgacgagata ctatctgcta
     1021 tgggcattat tgttttgcag taaaaattta cggtaaaaac ggtagccttc ctaccacaat
     1081 gctgatcacc atatatgcgg caatacagcc gtaagcaatg taatctctct tatgatagat
     1141 cagcggcttc atcttcgtcc tgccctctcc gccgcgatag catctggcct ccattgccat
     1201 ggcaaggtcg ttggctctgc ggaacgcgga gataaacagc ggtactaaaa ggggcaccat
     1261 tgccttggca cgtttgaata tattgccgct ctcgaaatcc gcacctctgg cgatctgcgc
     1321 tttcatgatc ttatccgttt cttctaataa tataggaata aaacgaagtg caatggacat
     1381 catcatggcc acttcatgta cgggcacctt gaatactttt aagggtccca tcattttctc
     1441 cataccgtcc gtcagattgt tcggtgtggt agtcagcgtc atcacagagg atccaatgat
     1501 cagcagcgtc aggcggattg ccatggaaat ggccatcttc agaccttcct gcgtaatggt
     1561 cagcttccag atcgtgaata acggtgtgcc cggtgtcaga aagagattga acaccactgt
     1621 gatcagcatg agcataacaa tggtcttcat tccctttacc atgaatttaa aaggta
//
LOCUS       DAWEJW010000222         1669 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_16713_length_1669_cov_48.094176, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000222 DAWEJW010000000
VERSION     DAWEJW010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16713_length_1669_cov_48.094176"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          371 a          433 c          341 g          524 t
ORIGIN      
        1 ctaccagttc cttctgtgcg tcggtcagtg catcccatgc tgccttggca tctgcgcact
       61 gctggtcagt attttcagtt ctctcctgta catagatagc gtcgatcagg gctgctactt
      121 cgtcagctgc tgcctgatct gcatcttcgt tgctctcggt agctgcggtg gttgtctctg
      181 ctgcctgtgt ggtctgtgcc tcagtgcctg cctgtgcagt gctttctaca gttgcctggg
      241 tattaccgca tccggtgatg catacagcac ccagtacgga tgccagtact actgctacga
      301 ttcttttttt catgtttcct cctctgacag atcttctgtc tcttttgatt ttggggcttg
      361 ccccggtatt cttaaatatg taagtcagaa acatgttttt cgcaacaaaa acaaaaaccc
      421 tttcctgtat caggtaaagg gtttgtacac ataagaaacg aatgcaaaaa gcttctgtcg
      481 cttctgcatt ccacacgtac aaccagttac tcgtggcttt gaaacaaatg tttctgtaca
      541 gcattcaggc aggtctcccg gcttgaagat cattgcatcc gccatcttcc cggtctccca
      601 gtgatctatc ggcttcagct ccctaattac ggtgacgagt tcgtgcagga cttacacctg
      661 cttccctttt caccggagcc agctacttac gtgactgctc cggcacctga atactccata
      721 ttcaattgtg gctactatat cacagatttt ctgaaattgc aatgtcaatc agtcatctgt
      781 tccaccggct gtcgggatca gtatacctga aaattagaga ttggcgtcat tccgggcata
      841 attcaggatc atttccttta aaagcagcag atcacctgta taatccttgt tgtccgttgc
      901 tccccagata aaaccacctg cgtaatgggt acacgctgcc atcatggtat gcaccgtcac
      961 acgcatgaac tgttcctccg gaatatccgt gcggaaggtc ttgtcctctt ttgccttcgc
     1021 atacatcata tgaagtcttg tgtcgacgga attcagggat gcgtggaaat tcgccagttc
     1081 ctcctgccgc accgtctggt gtgttatata attgttgaag ttgtcattat actgcagcag
     1141 atccttatgg ttctgataca tgccgatata actgtccagc gtaaagataa atcttccgat
     1201 ggccggaata tcgtgtatcg aagagattgg tcttgcctca tccaacttgt ccagataagc
     1261 cttccatttc gatgcgcaga ccgcaatagc cagctccgtc ttattgttgt aacagcggaa
     1321 caatgtggca cgtcccacac cggcttcttt tgcgacctca tccatgctga ccgcttcgat
     1381 tttcttttcc acaaaaagcc ggaacgccgt atcgatgatc cgctggttgc gcccttgctc
     1441 tttatccatc tgtttctcag attttcccat ttttctcttc ctttttcata cattcttact
     1501 gttcatttta tggtaaaagg ctgtgactgt caacgactgt aaaaagctaa tgtaaaagtg
     1561 ccttgcagat tcctacgaat tttcttgtat ttaaaacgcc aatatcggtg aaaaaaagta
     1621 aacgtcaaat cctgcgcctt ggctaattag tttttttatg ccacactct
//
LOCUS       DAWEJW010000223         1644 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_17020_length_1644_cov_26.498427, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000223 DAWEJW010000000
VERSION     DAWEJW010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17020_length_1644_cov_26.498427"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          464 a          327 c          494 g          359 t
ORIGIN      
        1 ggcagaactt gtggacctat tgaaaaccta tgccaatcat ccctcttttg taatgttgac
       61 actgggcaat gaattgcagg cacaaggtga aggcagagat cggatgcggg aactggtcag
      121 aacggcgaaa aagatggatc cgaccagact gtatgcgaac ggatccaatg ctttttacgg
      181 agaagagggc tgtgacccgg agagtgattt ctatacttcc cagagctgta aggatgtggt
      241 gatccggggg acgttctccg ggatgcgggg atatatcaac gaaaattatc catcggcgga
      301 tcacacttat gatgcggcga tggcagagat ccgcaaaagc tatacaaagc cggtattcag
      361 tttcgaggtg ggacagtttg aggtactgcc ggattttggg gaactggaaa gctttcaggg
      421 aatttcggat ccggtgaatc tgaaactgat tcggaagcgc gtagaggaac ggggactatt
      481 gcctgtatgg aaaaagtatg tggaagccac cggagaactg tcgaggctgg cataccggga
      541 agagattgaa gcagccatgc ggacgagaga gctttccgga atttcacttc tcggactgca
      601 ggatttcccc ggacagggga cggcactggt tggcatgatg aactctcata tggaatcgaa
      661 accgtatccc ttcgccagac cggagcggtt ccgggagttt ttcagagaga gccgtatttt
      721 cgtgaaattg ccccgctata cctatgaaga gggagaatat ctgacagcgg aagtagaagt
      781 ggcaaatttt ggaaaagaga gcatcagcgg tgagcttgtc tggacgttgg gttaccggga
      841 ctgtgctgtg aatagcggaa gaggagagct tcgatgcgac cggacagaca ggcaaaaaaa
      901 ctgtcctccg gggacttata cttcactggg aagactggac attcctctga aggtcaaggg
      961 agtatcggcg gcactcatac tgacggtgag tatcggtgac tgcaccagca cctatcctgt
     1021 gtgggtctat caaaaaacaa gcccggaatg tccgaaaaat gtatatgaaa cgagagtatt
     1081 tgatgagcag acgagagcag ttctgcaaaa cggcgggaga gtctatctgt ctccggatgc
     1141 agataaagag agcctgccgt attccataaa aacacagttt acgacggatt tctggtcggt
     1201 gggaactttc acggatcagg aaggtggtat ggggcagctg atcgatacgg agcatccgat
     1261 tttcaaggaa ttcccgacag attttcatac agactggcaa tggtggatca tggcaacgaa
     1321 gcgggcggtg gttctgccgc atcccatgaa gacgatcatt acggagatgg acagctatgc
     1381 attcttacgc cccatggcac agatgataga gtttcgatgc ctgaagggaa aagtattgtt
     1441 atccaccatg gaattgcata agagtcagca gtatccggag gcacgtgcat tgcagacggc
     1501 gatttatgaa tatctgtcgg gagaggattt tgaacccgcg gaggaaataa cggaagaaga
     1561 gttaagcaca ctggtgcgag gttaaaatta tatcgggcgg cggaaagaaa gctgcacgga
     1621 gataaaagga ggaaaccatg aata
//
LOCUS       DAWEJW010000224         1613 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_17423_length_1613_cov_40.474968, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000224 DAWEJW010000000
VERSION     DAWEJW010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17423_length_1613_cov_40.474968"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          397 a          428 c          353 g          435 t
ORIGIN      
        1 ttataccttg ccaagggcta cggggatttg atagcatttc tttactaact ttccagttaa
       61 ttaccgtttg atatcgccgt cttcggcatc ctcttctgtg gtaatggcaa aagcgatatt
      121 ggtggcatgg tctgctactc tctccagtcc ggatacgata tcggagaaga tcatgccggc
      181 ttcgggagaa cactctccct tggtcagacg ctctacatgt ttcttctgca gttcgcgttc
      241 cttctcgtct acctgatcct ccagagtaac gatctcctgc atatgggatt catcgctctt
      301 ggcgaacatt tctacagcat aacggatgat cttgttgacc atttccagca tatctcccag
      361 ttccttctgg gcttccttac tgatggaaac gccttcttcc ttacgctgtc tggcagcatc
      421 cgccacgttc tccgcatggt caccgatacg ctcgatatcg ttcaccacat ggaacagggc
      481 acccagactg ttcaagtcct cgatgggcag cgtggtctgg ttgatcttca ccagataatc
      541 cgtgatggca tgattcagga agttgatatt tttctctacc tcgtagacct ccgcaatgtc
      601 ctcttcatcc aacgtgatca gggcattcat ggcacggttc agattttcct ctgccagaga
      661 agccatacgc tccagctcct tgattacttc taccactgct gtggcggggt tgaacaccac
      721 tttgtctcca atatatttca gctgatagct ctctctgtaa cccaccttct ggtcctcgcc
      781 gggtacgatc aggtaagtca tcttcaccag ccagctggtg aacgggaaca gcatgattac
      841 ctgtgcaatc ttgatcagcg tatgtgcatt tgctacgaat cggccattgt ccgcagagat
      901 ggacttgatc agttccacaa tctggtctcc tgccacaaac agggcaatgt agatgatcac
      961 tgtaccgatg atattgaaca gcagatggat cagtgctgct ctcttcgcat cctttttacc
     1021 ggtcatggaa gctaacattg cggtagcaca ggcaccgata ttacatccca ggataatata
     1081 cagtgtaata ggcagtggca gcagatcctg attggccagc agcaatacga tactgaccgt
     1141 tacggaagag ctctggataa tggctgtcag ggcaaatccc atcagtgtgg ctaagaacgg
     1201 attcttcaaa gacatcagca gatttaccac ctgcggctca ttcttcatgt tcgccatagc
     1261 ctgggacatg gtgctcagac ccacgaacag aataccgaat cctacaacca cttccgccac
     1321 tttgcggact ttttcctttt tcgtgaacat catcacaacc acgcctacta atagaatcag
     1381 cggtgctatt ttcgataaat taaaagatac cagctgtgaa gtgatggtgg taccgatgtt
     1441 ggcacccaga ataaccccag ccgcctgata caggttcatc agaccagagt ttacaaagct
     1501 gacgaccatg accgtacatg cagaagacga ctggatgata ccggtaaaaa taataccgac
     1561 gatcataccc atgaaacggt tcgtggtaaa gatttccaga atacgacgaa gtc
//
LOCUS       DAWEJW010000225         1540 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_18386_length_1540_cov_51.822896, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000225 DAWEJW010000000
VERSION     DAWEJW010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18386_length_1540_cov_51.822896"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          355 a          380 c          412 g          393 t
ORIGIN      
        1 gacgtctgct tacggcctgc ttattcttac ttgctgtcgt gactactggg ttattcatta
       61 atcaggaagg aaacaccaat ccatggcaaa taacatttac cgcgttccta ctatatatag
      121 tgatatcaaa aaaacgactg aacctctctc agtccgggta ccgggttcta agagtatcac
      181 gaaccgcagt ctccttctgg cgatgctggc ggaggggaag tccacgctgc gtggagtgtt
      241 gttctcggat gattcgagac attttcttaa gtgtgtgcag gatctgggat ttgagacgac
      301 ggtggatgag gagagctata tcgtcacggt agaaggactg ggaggaaagc ttccctgtga
      361 agaggctacg ttaaatgtgg gaagtgcagg aacggcagcg cgttttctga cagctacgtt
      421 aggtgtctcg gaaggtgttt ttcacatgga ttcttcggag cagatgaaga ggcgccccat
      481 ggatccgctt ctgtcctctc tggcagaatt ggggtgtgag gtccgctgtg aaggagaaga
      541 tgggcatttt ccttttacgc tgaccgctca tgggttcgga caggatcata tctccatcga
      601 tatcgggcac agcagtcagt ttctgagtgc gctgctgatt gcttcgactt tgtcttcgga
      661 agattttatg attcaggtag agggtaccca tgggatggcg tatatcgaaa tgacacagaa
      721 gatgatggag cagttcggtg tctgcgtgga gcgtcccgcc gcagatcagt tccgtatctg
      781 caccggtcaa aagtatcgtg cactggatta tcagattgaa ccggacgtct ccgccgcctg
      841 ctatttttat gcaatgacac cgttactggg tattcccgta tgtgtggagc atgtgcactt
      901 tgaaagtctg cagggagatg tggaattctt acatatttta gagaaaatgg gctgtaccgc
      961 acaggagacg gaaaatgggg tactcctgct gcctcccgca gggcagacgg ataccggaac
     1021 gcaggttcct gccttccatg gcatcaccgt ggacatgtct tcctgctccg atcaggccat
     1081 cactctggca gctattgcgc cttttgctga cagtcccact tgtattacag gcatcggtca
     1141 catccgtttc caggaaagtg accgtatcca tgccatctgc acggaactga cccgcatggg
     1201 catccactgc gaagagacac aggacagcat cactatctat cccggcatgc cgcacccctg
     1261 ctccgtagcg acctacgatg atcaccgcat ggccatgggc tttgccctga ccgggctccg
     1321 ggccgaaggt atcgtgatcg acgatcccgg atgctgccgc aaaactttcg agaactattt
     1381 tgaagtactg gatcaggtga ttgcgaagat aatcgaaatg taggaattca caaatcatcc
     1441 cgatataccc gcatcacatt tccccagaaa atcccatcca gctggttttc cgtaaagccg
     1501 gctgttttta gtctgtccca caataccggc attgcctccg
//
LOCUS       DAWEJW010000226         1533 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3474
            NODE_18487_length_1533_cov_47.219892, whole genome shotgun
            sequence.
ACCESSION   DAWEJW010000226 DAWEJW010000000
VERSION     DAWEJW010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996986
            Sequence Read Archive: SRR8800847
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18487_length_1533_cov_47.219892"
                     /isolate="gSpreadC_ID_3474"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          414 a          350 c          462 g          307 t
ORIGIN      
        1 ggcatggaga acggggtaaa agaattgaag cttttgtccc cggaggaagt ctgggcaatg
       61 gagccggcgg tgtcccgtga catcgtagga gccctgtatg cccccaccgg tggtatcgtc
      121 tgtcccttcg gactgaatat tgcgctggca gaaaatgcgg cggaaaacgg cgtagagttc
      181 tatcgtgacc acaaggtgac agctatcgtg gaacagagac cggtatggac cgagaaccca
      241 cctgcgaagg aaggacggat gtatcaggtg caggtggatg gtgagatatt cgtggcaccc
      301 atcgtcatca atgcggcagg tgtctatgcg gatgaaattc acaatatgat ctgtgaggag
      361 aagatccgca tcgtgcctcg cagaggcgag tatcgtctga tggacaaaaa ttgtggcgat
      421 ctggtaaatt ccactatttt ccaacagccc tctaaaatgg gtaaaggtat ccttgtaact
      481 cctactgtcc atggcaatct gctggtaggt ccgacagcgg aggatattcc ggataagcag
      541 gatgtggaca ccacggcaga aggactgacg aaggttctga atctgggaag taacagtgtg
      601 gatgcgctgg acggcagaca gaccatcacc tcttttgccg gactgcgagc tcatctggta
      661 cacgaagctc ctgcggaaaa atcggatttc cttattgaag aggcagcagg gcatcccggg
      721 ttcatcgatg tggcgggtat tgaatccccg ggactgacca gtgcaccggc aatcggtgag
      781 tatgtggcac ggattgttga aaatatttat cccgcagaac gtaagacgga ttttattgac
      841 agcagaaaag gcattcccag catggcactt gccacggaag aggagagaga agcactgatc
      901 cgggaaaatc ctgcgtttgc caatgtgatc tgccgctgcg agctggtgac ggaaggagag
      961 attctggaag cgattcaccg cccagtagga gcaacgacac tggatggtgt caaacgtcgt
     1021 accagagccg gtatgggcag atgtcaggcg ggcttctgct ctcccaagac cctggagatc
     1081 ctggcgagag aactgcagct ggatccggca cagatcacta aggaaggaac cggctcagag
     1141 attctgaccg gtaaaaataa agacaccgca cccggagagg gcggaacatg gaagaggaca
     1201 caggcgcctg tcggaaagga ggcactccat gagtaaacgc acagaagata tcgtcatcat
     1261 cggaggaggt cctgcgggac tggcggcagc gctgtctgcg agaaaggccg gctgtgaaaa
     1321 agtgctgatc ttagagcgtg acagagaact gggaggcatt ctgaaccagt gtatccacaa
     1381 cggtttcgga ctgcatactt ttaaagagga gctgaccgga ccggaatatg cggaacgtta
     1441 tattgacatg gcagaagaag caaaaatccc ttatctgctg aataccatgg taacaaatat
     1501 ttccctgcgc tccgatggac gcaagaaagt cac
//
