LOCUS       DAWEMR010000001        56989 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_260_length_56989_cov_11.161819, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000001 DAWEMR010000000
VERSION     DAWEMR010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 56989)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 56989)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..56989
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_260_length_56989_cov_11.161819"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    824..835
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10296..10305
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    32128..32137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    43305..43314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        15704 a        13302 c        12023 g        15918 t
ORIGIN      
        1 cgctacctcc accttggcaa ggtggcgctc taccagatga gctaaatccg catttggtgc
       61 ttccggacgg aatcgaacca tcgacacgag gattttcagt cctctgctct accgactgag
      121 ctacagaagc aaaacaatgg cgaccggaat ggggctcgaa cccacgacct ctagcgtgac
      181 aggctagcgt tctaaccagc tgaactaccc ggccatatga gcggacttta taagtccgct
      241 tggtgggaac aacagggctc gaacctgtga ccctctgctt gtaaggcaga tgctctccca
      301 gctgagctat gctccctttt agctggtttg ttgttttcag gactttgttt tgtgactttg
      361 tttcgttgtg tccctcacaa cagtatctat tatacactat cccaccctgt ttgtcaacac
      421 tttttttgat attttttatg ttttgtatca ttttacaata ccagtccgat atttttaaaa
      481 acttatgtgc atttgcaaca tcaaagccgc atttttcgcc ttgaaatggg cttttttcat
      541 caggcttccc cccaaaaaaa atccgccgag ccacacgccc gacggataaa tattctcaaa
      601 aattatttct tatcaagctg ttttttgagc ttttcctgct cctgctccat aagcttcata
      661 agttcctcca aggaagctga cgctgtcttt gtctgctgtg caggctttgc cgcaggtttt
      721 gcctgcacag gctttttctc aggctcaggg gctacagtac cctcagttga agctgcctcg
      781 tcaagaagct gtgaagctct ctcgtagtaa gaacggtcta tctnnnnnnn nnnnngtttc
      841 ctcgacctct tctacttcct cgataacttc ctccgtttca gccttctctt caccctcagg
      901 ctcagccttg tggtgagaag ctatctcgtc gtcaacgtgc ttgcgatgtt ttgcgatctg
      961 ctcgccctcg ttctcgccgc cgaacaggaa atcgtcaaga gtaacgctct ctgcaggttc
     1021 ttgctcgtca tcgtcctctt cctcatcatc tgaagaatga gtagctatag caattataag
     1081 ctcgataaca acgagcagga atataggaac aacagcgcac actatcatac cgaatgttga
     1141 cgacataaac gtaagcacct tgcctgctgt catgtaatat gtagaagcta cgcccacaac
     1201 gttctttgag cttatctcat aaagctttgc agggtctttt tcctgataaa cagtgtaaac
     1261 aacgccgttg ttatcgtcag ttgaagtgat gtcataaagc cagaacacgc ttgttccaat
     1321 atcaggcacg ttctcgcaca caactgcctc gcctatcttg tctgtgctag gggtcatgtt
     1381 cgatgtgata acaagtgcgc cattaggaac tctgtcaccc atgccgtcac cattcattat
     1441 gtacaggctg tagcctgcaa tgctcggtgt aacgttcttg tttttgaaaa cacgtcccac
     1501 tcccacaact ataagcattt ccagaataat aaaaacaata aagaatatca gcacgttctt
     1561 gcctgctgat gacttctgct tagtagcact gctcatacta aaaataacat tcctttccaa
     1621 gatatatcca aaatatcata ttttatcgca ctaatacatt cattatatca caaagtgccg
     1681 caaatttcaa cacatttgag catatttttt gcttttgaga aaattattca tttttgtaaa
     1741 gtattatcgt gacagtacac agttcgtcat tttttatgac cgcatatttc gaggtagacc
     1801 aatctccacc agcctgactg tatgcacgcc tcagcacgtc aaagataaca ccactattct
     1861 catcaaagag agtatccaca gccttgtcat acatttcact gtcagcacat tttagccttg
     1921 catatccact gtcagccatt gcctccgcca cagaacggca catcatctga acgacatcat
     1981 caccgctttg agcatacaga ccgtttcttc tgtaataatt tgcccccgag gaaaatgcct
     2041 caggatagtt caaaaacttg ttgtcgtccg ctgtatggtc ttttgcacac tcggcgtcag
     2101 tgatgccaaa atagtcatag cggatatagt cctcccctgc atctgtcaca ggatcgtccc
     2161 aagtgaggtc aacgttcgtc cattcaccgt ctatcatgac cttgttccat aaatgtggct
     2221 gaacagctcc gccgtcatag gctttgcctg ccacaggtat gcactttata cccgccttgg
     2281 aacaaagtat ctgcattgcc tttgcatacc cctcgcacac agccttgccc tctacaagac
     2341 agccatatgc agaatagcaa ttttttgctg tatcgtcata gttacactca agaattatgc
     2401 tgtcatggaa atacagcacc ttgtcataat ccgaccagcc ctgctcaatg cctgcaagta
     2461 tctccccgac ctttttatcc agcttttctt tctcagcctg cgcttcctca gcggtcttgt
     2521 catatgtgac ctccacagat gtcacatatc catctccgtc aatgcagata gtatactgtg
     2581 aacccatata gtcgatataa ggacaagccg aagtaagcat ggagataaac gcaccgatat
     2641 cgtccttgct tataactccc tcagccaaat ccacagagct ttcaaatccg ctcagccctt
     2701 tgtaaagctg tgaaaacaca cgtttttcgt cagtacaaag aaaatcggcg atatactctg
     2761 ccgtgtcagg tatctcaagt gccgtaaaac tctcatttct gctgtcaagc cctgtttcag
     2821 tatgtctttc cgaactggct tcaacatacg catgagtgtt cacaaaagta ttttttgaac
     2881 ggtttttatt tttctctccc gttccgccta agctgcaccc cgatgtgcag ataagcactg
     2941 ccgctgtgag tgcggcgacc attttctttt ttttcaattt ttgtcctcga tttttacaca
     3001 aaattctgtc tgtatctttc agctcttagt ccgtgacctc ttcattatct ctttttatca
     3061 cagccctgag ccgatctagt ttcttttcat gtatcgacag caccttgaac tccacgtttt
     3121 cccaatgcac cgtcaccttt tcgttatcct caggcacatg gcccagcagg tccataacga
     3181 atccgcctat agtatcataa tccacctcgc cgtgatactc cttgccaaga gcctccataa
     3241 cgtcgtcaaa cgccgctgtg cctaccatat catagcaatc agaagatacc ttctgtatct
     3301 cctccgtttc gtcatcatat tcgtcctgaa tactacccac gatagtttca agcagatcct
     3361 ccatagtaat gatgccggct gttccgccgt aatcgtccac cacaactgct atctgtgtct
     3421 tatcttcagt gaactctttg aaaagctcac cacacttgtt gctttcaggc acaaaggtta
     3481 tatctctcat gaactccttt acaggacgtt ctttcttatc ctcttcaaac accagcctga
     3541 gcaagtcctt tgcgaatata acaccactta tgttgtcggc attgtcgcag taaacaggta
     3601 ttctcgaaaa gccctcacct ataacaagct tcacagcctc cgaaagcgtg gaattttctt
     3661 ctatggcaac catttctgtg cggtgtgtca taacttcacg aacttcgata tcgtcaaaat
     3721 caaaaatgtt gcttatcatt tccgcctgac tttcctcgat accgcccgtt tcgtttacag
     3781 cgtcaaccat catcaggatc tcttcctcag taacagcgtc cgccttgttt gttgacttaa
     3841 caccaaacag ccttaatatc cctgcggata ccgctccgct cactatcgcc aatgggctga
     3901 acaccgccaa aagcgccttg taagccacac agctatttag aataaatctc tcgcccacct
     3961 tgcctgaaat gcaaagcttt tttggtatgc ctacgccaaa agtgcatata acaagggcaa
     4021 gcagacagct tattataaca aatgaacata tgccgataat ataatacgaa gcctgagtat
     4081 atacatcaaa aagcttgtga agcttatttg aaagcggtgc ataaaagtag atcgctccta
     4141 ccaccgaaac tgcgattatc ataatagaac gtgcgatgag gtttgtcgca actaccctgc
     4201 ccgttctgcc aagcaaagcc gcaagcctta ttgccttcgg gtctttgtcc tcctcagcca
     4261 ttttctttag cttcgcgtca ttgaactcca cagccgcatt ttcgcacaag gtaagaaaag
     4321 ccataagcaa tattaccgcc gcacaaacac ctattcccgt ccacagtcgc ccgacatcgt
     4381 ccataaaaaa aataccgtcc ctttcacata ggcgaaaaag ctcgcctttt aatcatatac
     4441 tatttattat aggcaacata tgcttctttg tcaatagata ttgtcacaat ttttcacatt
     4501 ctgcctgaat ttcatatggg caattttagt tatgaaaaaa gcagacgagc cgcaaagccc
     4561 atctgcttac atcaaacaat attatttctc tgccgcacct atcttggaaa ctgcgtccct
     4621 agtagtgttt acgtccttaa ggctctttgt aagcacctca gccgcctcag aaagcgactt
     4681 gttaagattt tcagaaagtg cctcacggat agccttgtcc ttagccttag cctgctcaac
     4741 gatatcagcc gcctgcttgt tagcttctgc agctatctgc tttgcatagt cagccgcctg
     4801 cttgatgatc tcctcctgag ccacaagctt cttagcctcg tccttagcct tctttacgat
     4861 ctcctcagct tccttgttgg cctcagtaag gatatttttc ttatctcttg tcatatcctt
     4921 tgcacgcttt atctcacccg gaaggttaag tcttatgctg tcgatgtatt cacgcatctg
     4981 ctcaacatcg atcatttttt tctgtgaaaa cggtacgctt gaagacttgt caagcagttc
     5041 gtccataagt tcaagtattt cctctaccgt cattttaaac accctttcat tactgatcta
     5101 tattattggc aggcttatag agcctgtcag tgatcttgct ctgtatctgt ttcggcacaa
     5161 agcctgtgat atctccgccg aagcttgcga tctcctttac aacgcttgac gaaaggaaca
     5221 tattctctcc cgtagtggtg agaaacaccg tttcagcgtc attgtaaagc tttttatttg
     5281 caagagccat ctggaactca tactcaaagt cagacacagc cctaagtccc tttacgatag
     5341 cattagctcc tgttttttgc agataagccg ccagcagccc gttctaacta tccaccacca
     5401 cattatcaag gtgcttcgtg gcttcaatta tcatttccat tctttctgta ggtgtgaaag
     5461 cagcccttga tttattagca ttaaaggaca ccagcactat caccttatca aaaagctttg
     5521 aagctctgtc gattatatcc agatgaccca gcgtaacagg gtcaaagctt cccgggcaaa
     5581 cagcgatacg catcaatagt ccacttcttt cattggtatc ttatagaccg tcaaagcggt
     5641 cttaccgtat ttatatctct tatgaagctt aagtctgccg tagctttctc caagctcaag
     5701 ctccttttca tgctcgcaca caatgatagc accatcattg agcttgctct ccacaagctc
     5761 caaagccttt tcaactatgc cctttctgta aggcgggtcg ataaaaacta tatcaagccc
     5821 ggctttggca actttaagat attccagact atccatgcaa agcacacggg attccttatt
     5881 gaaaccacat gaatccacat tatccttgac gatagccaca gcctcaggat tgctgtccac
     5941 gaaaacgcag tgactagccc cacggctgag agcctctata cccatttgac cgctgcctgc
     6001 aaaaaggtca agcaccgagc ttgctggtac atcaaactga ataatggaaa aaacagcctc
     6061 ttttaccata tctgtagttg gtcttgtatc caatgactcc aaagttttaa gcttttttcc
     6121 tctgcatgaa cctgtaataa ctctcataaa aaataactcc aaaataaaag tatacaatca
     6181 ttatactata ttaattacca tttgtctaga ctagtcgccg atattttttt attttttata
     6241 ccacacacaa aaagggactt cacagcggaa gtccctttag ttattttaat tacgtactct
     6301 aagaaacaag tcctcagtta agattacttt ctcttagaaa ctacgatagc tgcacctgcg
     6361 agagcgatac ctgcaagagc gatacctgct gttgcgcctg tagctgctgt tttgtcagaa
     6421 gctgcatttg aagatgtagc agttgttgtg cctgttgtac cagtctttga agaagttgtt
     6481 gcggctgtgc tgttagcttt tgaatcagtc ttgctgtcag acttagaatc tgtcttggaa
     6541 tcatcctttt tgctgtttga atctgtgcca gaagtagcag gagcaacagg aacagcgtta
     6601 gcagcccata tccagccctc gtttgttgag ccgtcatcga acttctgagc ctcagcgttt
     6661 acagtacctg actgagcgat agaaattgtg taagagccgt cgccgttgat agcagtatcc
     6721 tggcagtcaa cgtttgtgtc gaatccacct gcgccacctg cgatacctac gcacttgtta
     6781 gggaagtaag ctggctgtgc aaccttatcc ttttctttgt catctggatt tctgaaatcc
     6841 caagtgccgt cagcttggaa agatacacca gcattacctg ttgtgtcacc aagaccttca
     6901 attgtgaagt caatagtgat gtactcacca gcgcctgcta cagtgtagtc agtgatctct
     6961 gatgtgaact tgctgttcca cttgttgatg atctggaagc agagcttgtc tgaatcagac
     7021 ttgccaggag cagcacttac gttaaactcc tgtgaaccga tctttacctt aagatcctta
     7081 caagtaacga cctgaccatt tacaagagcc tcatcgttat cgttgtactc acatgggata
     7141 tctgtagcga tgaacagata gttgtaagct gttgaatcag cagcgaaagc tgttactgcc
     7201 atagctgttg tagctatagc tgctgcagcc atgcctgcga aaatctttgt aagtttcatt
     7261 tctaaaactt cctttcttaa ataactttaa gtatattgta ctttattttc cgaaaaattt
     7321 caatcggctg atttaacaaa tatgataacg ttttcttatg caatcacaac aatttgtact
     7381 attgattttt agaagtatag cccaatttcc gcactttgca aaactttttt atcttatgta
     7441 aaaatactaa accgtcattt atcaagcttt ttcttctgca cctttccagt cactttcagc
     7501 accatacctc taggagcaaa tttagcccct gcataaagtg cctttataga cttctcaggg
     7561 ataatgatga gttttctgcc gaacattccc acaagagcct tttttgcaca aaactgtggt
     7621 gttatggagc ttacgccgaa cttgcagttt gcaacgtcat tgaactccgt atccacagga
     7681 cccgggcata ccgcacaaac gtgtaccctg ctgtttctcg ccctcaactc ctcatatatt
     7741 gcgctggtaa ggctcacaac ataagtcttt gaagcatagt atgttgccat catagggccg
     7801 ccgtacataa gccctgccga ggaagctata ttcattatat aacccctgtc agcggcaaca
     7861 aagtctttca aaaacagctt tgtgagcata tgcaccgctg taacgttggt gtctatcatt
     7921 ttaagctcag tgtccagagg aagctcgtca aagctgccca ctgcaccgaa acctgcgcag
     7981 ttcacaagca ctgatatctt ctcgttcttt gtttcctcat aaagccgcag acattcttcc
     8041 ctgtcggaaa catcgcaaac tatcaccctt gtattgtcac caagctcctt cgcaagctct
     8101 ctgagccttt cctcacgtct tgcgctgatt ataacacgaa agccccttgc agatagatct
     8161 ctcgctatct ctctgccaat tcccgagctt gcgcctgtta ccaaagccgt cttagccatt
     8221 tatatcatcc tccacccttt tcagggatat tatttgattt ttgtcatatc agtatatcac
     8281 attagtatca ccctgtcaag tgtaagaaag atgtaaaata cgcagaaaag catcgcaaaa
     8341 aactgaataa aaatgtcaaa atctcttgac atttacttta aagtatggca taatgttaat
     8401 ataattttaa gaaatagaat aaaattaaac ttgaaagaag tagggagaat ttatgaataa
     8461 ctatcaaacg ggcaatataa agctcgaaaa gctcgcaaaa gaaaattcag cttttcaaaa
     8521 ctgtcagcct gatatgtctg acgtaaactt tgagataggc aaaagcagca gcacagttaa
     8581 aatatttgcc gccatagcag ctttcgcatt gatatatggc ataagatttt tcttacgccg
     8641 ctatgtcgga aacccgaatg gggcgatgtc tgccggagat atcgtttttt ggtgctttat
     8701 aggcttgata attgtccttg tagttgtgac aacggtcaag gaaaagaata gtcccactat
     8761 ttcggttatg ggcaaaaaac tgttctataa cggaaactgt tggtcaagcg atgagatatc
     8821 tcatgttaaa tgcacaaaat ggttagagag agtcgaagtg tattcatcag gcaaaaaagt
     8881 cctgactttc ccatgggaaa aggataactc cgagcttttt atagcttggg tgaaaaagtg
     8941 cgggataaaa tttgaagata accgtatgca cttattctga taaaaatcaa aaaaatatat
     9001 aaaccaaaga acaaggagat aaaaaattat gagttgtacc ctttgcatac ttgccgcagg
     9061 tcttggaagt cgcttcaaag gcggcataaa acagcttgaa cctttcggac caaacggtga
     9121 gataataatg gattattcta tctatgacgc cgttgaggca ggctttgaca aggtcgtatt
     9181 cattataaga aaggatatcc gtgatgcctt tgataaaatg ataggcgacc gtatctcaaa
     9241 atacgtcaag atagattacg tttatcagga gcttgactgt cttccggagg gctattccgt
     9301 acctgaggga cgtgttaagc cgtggggtca cggtcacgca gtctgctgtt gcaagggcgt
     9361 tataaatgag cctttcgcag taataaacgc tgatgatttt tacggtaagg aagcttttgt
     9421 gaagctccac gattttctct gcaatgaaaa ctcagattgc aaaaagcttc atctcggcat
     9481 ggcaggcttc atactgaaaa acactctgag cgacttcggt gcagtaaaca gaggcgtctg
     9541 tgaagtgaaa gactctatgc tcacagacgt tgttgaaacc tacaatatcc gccgtgagaa
     9601 tgacggcaag gtctattcag gcaaggaaga aacagccgag cttgacgaga actgctatgt
     9661 gtcaatgaat atgtgggctt gccctgacgg ctttatagat aagctcaata gtatgttcac
     9721 agactttatg aaagaaaata aagacagcct gacagcggag tttctcctcc ctgcgggtat
     9781 cgatataatg ataaaaacag gtattgcaga ctgcaagctt ctggaaactc acgataagtg
     9841 gttcggcgtt acatataaag aagataagcc aaccgttaaa gccgatatcg caaagcttat
     9901 agaggctggc gtttatcctg aaaagctttg gaaataaaac tttttcaaac aaataataca
     9961 gaataatttg tcaacaataa gtcgggcaga acaaattctc ccgacttttt tgcgtctgtt
    10021 tgtaaaaagt gaagaaaatc cttaattcga ctaaaaagca cttgaaaaag gctgtaaaat
    10081 gtgttatgat aatctttgaa acggatctgc atttttgcag gtcaggagag ataatataag
    10141 aggtagtata atggagagcc aacacggtag caatttgaaa aatataagaa aaaaaagagc
    10201 cacagcacag caaagcatag tgtcattgag ttgtcttgtg tgcatagcat ttgcggcaat
    10261 atggggtttc catgcaggaa gcggcaacag cagggnnnnn nnnnnagaga acgaaagctc
    10321 cgagagcaat gcggacagct cttctcaggc agaaagcatg gtcgatgaga gcggcataga
    10381 tgaaagcctt actgacagct cagttaccca tgatgacgcc gacgaccttt ccgacgcagt
    10441 tttcataggc gactcacgaa cagttggtct gcaaaacaac tgcgataaac caaaggcaac
    10501 gttcctttgc tctgtgggaa tgcacatcga caccgttatg acagaccaga acatcaccct
    10561 ctcaaacggc aatgcaggca ctgttatcga cgctcttgga gaaagacaat atggcagagt
    10621 gtatataaac ctcggcacta acgagcttgg ctggccttat atcgacacct tcaaggatta
    10681 ttatacacag cttatcacaa aaattcagga gatacagcct gacgctgtta tctatgctga
    10741 atctatcctc cctgtaacag cttcaagaag cttgcagggc gacgccataa ataacacaaa
    10801 tgtagccaca ttcaatcagg ctatctcaga ggtggcacag cagacaggta taaattatct
    10861 tgactgtacc tccgctgtag caggcgcaga cggcactctt ccggaggaag cctcctcaga
    10921 cggaatacac cttatgagcg aatactgcga caagtggctc aactatataa tcgacaacac
    10981 ataagaaagg aaagaagaat tatgaaaaca aagaagatac tctgcactct tatggcagtt
    11041 atgtgcattg gcacagcagc acttacaggc tgcggttcag actctgacag cagcacagca
    11101 ggctccgcaa attcagcggc agccgctcag acaaacgaaa gcgttgacgt aacagccgtt
    11161 gcggacaagc tcaaggacgg cataaagttc aaggacgagc ttgcagagct tgacgaagca
    11221 aagatagaaa agataatcgg catatcagca gacagctata caaaggctaa ggtctatgta
    11281 agctcatcag gaggaacagc cgaggagata gactgctttg aagccaagga cgcagacagt
    11341 gcaaaggcta tcgcagatac acttactgca agagtcgaaa gtcagaaaaa ggtttttgaa
    11401 aactatgttc cggaggaaat gacaaagctt aatgaccctg ttcttgtaac caacggcaat
    11461 tatgtttacc tctgtctttc aaacgataac gataaagcaa aagagataat aggctaggag
    11521 taataaatat atgctttttt caagtacaac atttctgttc gcattcctgc ctattgtact
    11581 gataacgtat tatatcgtcc cacgcataac acgcaatctg gtgctgttta ttttcagcct
    11641 tgtgttttac gcatggggcg aacctgttta tatcgtcctc atgatagctt ccactatcgt
    11701 tgcgtatata acaggactat tggcagacaa acagcgtaaa aacgcaaaaa aatacactcc
    11761 gctcatcgca atgataggag cggtggtgtg gaacatcggg cttcttctgt tttttaaata
    11821 taccgacttt ttcatcggtg cggcaaacag cattttcggc acgcagataa agaccttggg
    11881 tcttgccctc cctatcggta tctcattcta cagctttcag accctttcct acgttatcga
    11941 cgtttataag ggcgaagtaa gaagtcagaa gaacttcctt gaccttgcca cctatgttgc
    12001 ccttttccca cagcttatcg caggtcctat cgtaagatac atcgacgttg cggaacaact
    12061 caaaaagcgt aacgagagca tagagcattt tgcacagggc gtgtggagat tttccatagg
    12121 tcttggcaaa aaagtcattc tcgcaaatac tatcggcgag cttttcgaca ctatctcaaa
    12181 ctcagcacaa tccgaactga gcgttgccgc aagctggctg ggcataatag cctatacatt
    12241 ccaaatatac tttgacttct caggctattc cgatatggcg ataggtcttg gcaaaatgtt
    12301 cggcttcgac tttctcgaga acttcaacta cccttacatc tccacctcta tcacagactt
    12361 ctggagacgc tggcatatgt cactttcaag ctggttcaga gattatgtct atatcccact
    12421 tggcggcaac cgcaagggca aagcaagaca gtgccttaac atcatgatag tatggtttct
    12481 cacaggcttc tggcacggtg caaactggaa ctttatgcta tggggtgtgt acttcggtgt
    12541 aatactccta tgtgaaaagc ttttccttgg caaagccctg aaaaagatac ctgcattttt
    12601 ccagcacctc tatgcacttc tttttatagt cataggctgg ggaatattct cctatgaaga
    12661 tttcggcaag ctcacacaga acttcaaaaa tatgttcggc gcagggggac tacctctcat
    12721 aaatcaccaa acagtgttct ggctcgcaca gtatgcagta acaatagtga tacttgtgat
    12781 agcttcaaca cagcttccaa aaaagctctg ccttaagctt cagatcaagt gtcctgagat
    12841 atgcggctgt atactccaac cgctgttcgc attaacactt ttggcagttt ccaccgccta
    12901 ccttgcaggt aattcattca atccgttcct gtatttcaga ttttaaagga ggacggcaga
    12961 tatgaaaaaa caacttaaaa actatattat gatagtgtgc tttctgctgt tcatattcgg
    13021 ctttgcagtg gcaagcttta tttctgagga tcgtgaattc tccgaaaatg agaaccgcta
    13081 tctccagcaa gcccctgaat tttcatggaa aaaactcgaa aaaggcgagt tcacatcgga
    13141 tatcgaaagc tatatgtccg atcagatatt catgaaagac cagcttgtga gtcttaaaac
    13201 agtcaccgac cgcacgctac tgaaaaacta tcagaacggt gtatatttcg gcaaggacgg
    13261 ctattatctc caagactatc aggaaaacag acctcttatc gagaaaaata taagctgtct
    13321 taacgatttt gctgacagcc tcgataaatc cgtcgacgta agctttcttc ttgtgccgaa
    13381 tgcagtttcc gttatgtcag acaagctccc tgcggtcaca cagaccgacg atcagcttga
    13441 aagcgaaaag tatataagct ctatcctcag cgacaggata aacctctgct tcccatatga
    13501 ccagctcaaa gacgccgcaa agtccacgca ggttttctat aagaccgacc accattggac
    13561 agccgagggc gcaaaagtgg gatttgacgc acttatgaca gccatgaatg aagatatccc
    13621 acaggtatcc tacaatatag aaactgtaaa ggactttcat ggcacacttt attcaaaagc
    13681 accgacagac ttctccgctg ctgacgaaat gcacttctac acaaaccctg acaacagcgt
    13741 aaaggtaaat tatgttcaag aaaacaaaca aacagacacc ctttttgacg attcattcaa
    13801 gaccaaaaag gataaatact ccacattcat gggcggaaac tttgccctca cggaaataga
    13861 aacaaacggt gaaagcgatg aacacgtcct tattataaag gacagctacg caaacacagt
    13921 tatgccatac tttgccgaca agtataagca catcacaatg atagatatgc gttattacca
    13981 catcatggat aagacagtca gtgaatatgt aaaagacaac ggtataacaa aggtcatttt
    14041 cctttataat atggactttg tgaacacaga taacaacttc atatggcttg aataattcag
    14101 caacaattag gggcggccat tggtcgcccg ctatttttat cacaccctca tataacgtca
    14161 acgtcaaacc cacagaaaga aggacataaa atgacactta ccccctacaa aagaaaagcc
    14221 ttttattatg aaacagaccg catggatata atccaccatt ccaactacgt ccgttggctt
    14281 gaggaagccc gtgttgacct tatggaacag cttggctgcc cattccacga aatagaagaa
    14341 cgtggcatag tttctcctgt cctctctgtt gaaacacact ataaatatcc cgtccgtttc
    14401 ggtgacgagt ttgaagtgcg ctgtaagctg tcaaagttca acggctgtgc ctacgagctt
    14461 gactacgagg tctttgacct taccactcag aaaacagcct gccttgccca cacctcccac
    14521 tgcttcacca gccccgacct taagcctgta agactaaaaa aacattatcc cgatatctac
    14581 gagaaattcc tatccgcata tcaggcaagt gaagaataag caaaaaaaat gtacagctcc
    14641 cgagtttctt ctcaggagct gtacttttgt gtaaaaaatg acaggcgtga cctgtcacga
    14701 agaaagcaat ccatcaacgc cctcacgcca cttttcaaag ctgcacaaac caagctttgc
    14761 ccagcgacat tccgagcata gtatatcaaa tgccttttta tccattttgc ttctcagcct
    14821 tgtcagcgca ctgcgataat tctccactga gtgacattct atctcagaaa gtctgcaaac
    14881 ctcgctgtct ttctgtgcca ccgttccgtc acagccgtca agcaaacaac cgtcctccgt
    14941 cctgtttggg cagtgctgac atatcatatc tccgctgcac acaaaccgaa ccgcagtgtc
    15001 ctcgctctcc aatctgtctt tcacagactg catattcacc gaaaagccgt cactgtagcc
    15061 ttttccctga aaaagcggca ggcacagcag atgatgaaat ctaagctcag gctctcttgt
    15121 aagcatagca acctgtcttt gcaagtctgc ttacaagctc gatgctgata aggtaagcag
    15181 caacaacaga gataacgtcc tttacaagat aaggaacaga aacaagaagg atagacttgc
    15241 cgatagagat attagcaacg atagcgtact ggatagcacc aagcaggtgg tcgcaagcaa
    15301 gacctgcgat accaagcacg atagcgatga ccttaccgct tatcttgtca gagcagaact
    15361 ttgcgccaag accgcaaaga agagccataa cgataaatcc gtaaatgaat ccacctgtat
    15421 accctacgat aacggagaaa ccgcctgacc agcctgcaaa cacgggcaca ccgatagcgc
    15481 ccagcaaacc atagaccaga gcagacacag caccgccctt ccagccaagg aaatatcctg
    15541 cgagagccac agcaaaagtc tggagcgtaa caggaacgtg agtaggaagc ggaatagtta
    15601 tctgcgaaag cacagcgatt atagccgtca taactccgat accgactatt tgttttgttt
    15661 tgtttttagc attgttattc attgtaaacc tatcctttac tttttatttg acaattaata
    15721 ttgtatcaca taccgaacct attgtcaacc aaaaatattt atttcggtat acaattataa
    15781 ggcgtcggta aacgatactt ttatacaaat ttaataacaa acagtgaaaa cacggcattg
    15841 cccttccgaa cgggacacag agggcgtcgt cccctacaat atttcgggca acttcctacc
    15901 gccgcaaaat cgctacactc ggtgctgtct tttacttcgt acttgagcac aactctgttc
    15961 agctttgcca attcctcatt cctcactcct cattcctcat tcctcatctc ccccgttttt
    16021 tgtgcatttc gcccttaaat cctactaaat tgatagaatt aatatcagtt ttcacaaaaa
    16081 tccgaaaaat catcttaaac ctattgacat tcctctatgg gtgtggtata gtatagatac
    16141 gctaaaacat actaaacgac taggattaat atgaatttga aatgaggcgt tgaaaatgag
    16201 tgtaaaaata ctgaaactga aaacagcgta cgattcacga atgtgtatgt gctgtatgtg
    16261 ttgctgttaa aatcagcata aagttcagta acaaaaaatt tttatcggat ttaaaaaaac
    16321 attaatttaa tgaaagaggt aatttaaaat gagcgcaata aacgaaagaa atcttaaatt
    16381 tgaaacactc cagctccacg ttggtcagga aacagcagac tctgtaacag atgcaagagc
    16441 tgttcctatt tatcagacat catcatacgt tttccataac tcacagcatg ccgctgacag
    16501 atttgctctt aaggacgcag gcaacatcta tggcagactt acaaacccta ccgaagacgt
    16561 ttttgaaaga cgtatcgcag ctctcgaggg cggcacagca gctcttgcag tcgcatcagg
    16621 cgcagcagca gtaacatacg ctattgagaa cgtagccctt gcaggcgatc atatcgtagc
    16681 tgcaaagaac atctatggcg gcacatataa cctgcttgca cacactcttg ttgattacgg
    16741 catcacaaca acatttgtcg atccgcttga cccagctaac tttgaaaagg ctatcaagga
    16801 gaacacaaag gctctctata tcgaaactct tggcaaccct aactcagacg tagttgatat
    16861 cgaagctatc gcaaatatcg cacacgcaca caatatccca cttctcgttg acagcacatt
    16921 cgctactcca tatctcgtaa gacctatcga gtacggtgca gatatcgtta tccattctgc
    16981 aacaaagttt atcggcggtc acggcacaac aatcggcgga gttatcgttg aaagcggcaa
    17041 gtttgactgg aagaagtcag gcaagttccc acagctcaca gaaccaaacc caagctacca
    17101 cggtgtaagc tttgcagacg cagcccctgg cgcagcattt gtaactcgta tccgtgcgat
    17161 ccttctccgt gatacaggtg caacactttc accgatccac gcattcatct tcctgcaggg
    17221 tcttgaaaca ctgtccctga gagttgaacg tcatgttgag aacgcactta aggtagttga
    17281 cttccttaac aagcacccaa aggtagaaaa ggtaaatcac ccttcagtat cagacgatcc
    17341 aagccagcag gcactttaca agaagtattt ccctaacggc ggtggttcta tcttcacatt
    17401 cgagataaag ggcgacgcaa agacagctca gaacttcatc gaccacctga aactcttctc
    17461 acttcttgca aacgtagcag acgttaagtc actggttatc cacccagcat caacaacaca
    17521 cgcagagctt aacgaagcag aacttgcaga tcagggtatc aagccaaata ctatcagact
    17581 ttctatcggc acagagcata tcgacgatat catctacgat ctcagcgaag cattcgacgc
    17641 tatctaataa aaaccttgcc attacggttt ttaccttaaa tacagacctc aatatagaaa
    17701 cggctgacag gcgaaaagct tgtcagccgt ttttgtaccc acaaaaaaag cagggcaaca
    17761 acaagccacc ctgctccata aagcaaaaag tcagaaaaaa tccttcgcca caaggcgata
    17821 ccttaattcc tcactcctaa cttctcacta cttctccagc tcagagatct tatccactct
    17881 cctctgatgt ctgccaccct caaactccgt ttcaaggaaa acgtccacca gctcgatagc
    17941 caagcctgca cccagcactc tcgcaccaat acaaagcacg tttgcgtcat tatgcgctct
    18001 tgtatactta gcagagaaat agtcagaaca gcaagccgct cttattccct ttatcttgtt
    18061 tgcgctcatt gacattccaa taccagttcc gcatatgagt attcccattt cgtattcgcc
    18121 cgaagccact ttctcgcaaa cttcctttgc cttgtcagga tagtctactt tctctcctgc
    18181 ctgaatacca aaatcagtgt attccacacc cttttcgtcc aaatgtgcca atatctccgc
    18241 cttaagctca ggacctgcat ggtcacagcc aatagcaatt ttcatattaa tttcctccta
    18301 tttatcccta tgtgccttat ccttttccgc ctttgtagcc tgcaaatatg atacctccag
    18361 cctgtccgcc gaaaccatta tctcaggctg agcctccacc gcaagacaca gcgacgaagc
    18421 cctctgcaac ctgttcacat agcttgcaag ctgaacattt tcgttgtcag cgaaatattt
    18481 cgcctttatc tctgccgcac agtcaccaac aagcattgtc ttttcagtaa actctcccga
    18541 aaaaacttcc tcctcagagc aaactctgtc agcggatatc ctcgtgacag cgccgttttt
    18601 gcactgaaac tcgcctgcgt aaactatctt aggccttgcc ctcataacag aaattatcct
    18661 gccctcaaaa cctatgcagt tgtaagccag cgaaagcaga gtggaaactc ctgcgctttt
    18721 taaactctca cagccaaagc acattccctt tacagccgcc acgcctatcc taagtcccgt
    18781 gtaagacccc ggaccctttg caatagccgc acagtcaagg tcagcgtaag taagccctgt
    18841 ttcctctagt gcctgcttca ccataggcaa tatcacctgc gagtgagtga gctttgtgta
    18901 tacagacttc tcccaaagaa gcttctctcc gtccgtcact gccaccgaag cgacctttcc
    18961 gcttgtgtca atagctaata tcttcaaatc tatcatctcc ctcaggtgca gttatgctta
    19021 tctccctgct gtcctcgtca atgcgctgaa tatctatcct caaagcgtcc tcaggaagct
    19081 cctcagcaat attctccgac cattccaccg caagcacagt gtcttcgtcc atgtagtcaa
    19141 aaaagcctgt agtttccaga tcgtccgccg aatttatgcg gtacatatca aagtgtacaa
    19201 ggctgagcct gtcccctcgg tattcgttca ccagtgcgaa cgtgggcgaa gtcacctcgt
    19261 caccaagtcc catgccaatg gagatccccc tggtaatagt agtctttcct gcaccaagac
    19321 caccgctgta agcgataaga tcgccgcctt tcagccgtct gcctatctcc tcacccaaag
    19381 cgatagtttc cgccgcagat gatgttttgt atacctttgt caacctatca cctcatttga
    19441 ccgtcatctt ccgtgtagcc cacaataaaa aggctgtttt tattcataag ctgataaatg
    19501 ctctccacct ctgtcttgtc cgccgctgag ttgtagcagt tgtaaacata cagcctgccc
    19561 tcttctttaa cagcgaaaac cgtgtgaata ccactgaaaa tattcaaccc gatatggtaa
    19621 gaaactatca caggcttgcc ctcttcaaca gccgcctgag cctcatcagc cttataaaat
    19681 ctcttgtatc ccactcccat tgccttaaaa agtccgctta ttttcctagg attagacccc
    19741 agtgaaccgt ccgccgcaat aagagcattc ttttcaaaat cataagcaag ctttgccatg
    19801 tcaggctcaa agccctcgta tgtgagataa ttataagccg caatgacctc acagcctacc
    19861 cctgcgaacc tggtttcgcc aagcatcaaa tcgcacacag ggggattttc ctgcccgtat
    19921 ttcacaaagc cctctatctt tatgcccttg ttgtgagcat aattttccgc agccctgccg
    19981 ctgtcagttt ccttataagt gtcctcaaaa agccgccacc tcatatcgtc ctccgagctg
    20041 tcgccctgaa taccgctctc atcaccgacc tgcgagcttt cgtcaacttc cattctgtca
    20101 ctatccacca cagagctttc cgcagtagtg gtaaaagccg aatcattgag cctttcactg
    20161 tccgccttgt ttccgcatga actgagcatt atcccgagaa acagaatgac cgcaagaatt
    20221 ttcatcacag ctcacccttg tccttgaagt agtttagcat aagatccacc attctgccat
    20281 aggactgctt gccgtcctca acgccgttga gcttcagaga tttttccatg gccttgtcag
    20341 atatctcccc cactgcctcg ctgtcgaaaa cagtgtcctt agcctcatca acgctccgcc
    20401 agtagtccca gtttgcttcc atatcagccc acacctcgtc gcagatagag ctgtcaaatt
    20461 ctatcttctt atcgtcagaa gcgtagtcaa aaatcttgtt tctaacctcg ccaagcgccg
    20521 ccagatagcc cgaatagcgg taatccacat tgtccgatct atcgcacgcc ataaaggcga
    20581 taaagttcgc ctcgtcctcc tgaatgtagc ctttcgtatg agcaagctca tgacaaatgg
    20641 tgtcaggaag ctttgcctta tacatatcct gattgtagtt agcctccata gagaacggga
    20701 aatagatgcc cataaggtcc atctggctca taaagaacga gcattttata gctttcggag
    20761 tgacataaaa tcccccgagg tttttaaatt cactgcccag cccacgcatg gcctcctgag
    20821 ccgtcttgtc aaggtcagcg gtcaacacga attttccctc gctgtcacgc tccacctctt
    20881 tgctaagcgc atttatctcc gtaacaagct tgttgccaag agcttcaagc tccgtcctgg
    20941 tgtgctgttc agtgggtatg ccgttaagtt ccgcaaagct tgaacaatga taaataacaa
    21001 agcagttaaa agtctgcacc gtcaccacaa aagtgactat ccacatataa acaagcccaa
    21061 aaatcttcgc agttttcttt ctcttgcctt ttctgactat cacaagcacc agcatagcca
    21121 ccagcgaaag cggaataccg aaaaccgcta ccgcaataag caattccccg aaggaaaacg
    21181 gcaacagcga agtgagcctg ccatatgtat tttcccaaat tggaaaaacg tgagccgtat
    21241 accagtcgca gaacgccgta cttttccagg cgatcacgtt cagcaccacg ctgactgcca
    21301 caaggcaaac gcaggcaatg acctgccaag atagatgttt tttcttgccg ttctcaggca
    21361 ttttcacact ttccgcactt tttttcaccg ctgtatctgt cactgccgct cacctcggtt
    21421 catgtttttt tcactctgct atctcaacac acctgtttac ccgacttatt atctgcttac
    21481 ctacaaatta tcttatcgca caaatcgtgt aggggcgaac agcgttcgct cgtcttggtc
    21541 gcctacatgg tttgtataaa ttcaaacctc gtcgctccca aaaacgggac gtcgagggcg
    21601 ccgtccccta catttgccac gccgcaaaat cgctccgctc ggtatttact ctatcaaagt
    21661 gactcgttcg gtcggctatg ccaattcctc actcctcact taaacggctt agaacaatcc
    21721 gtagatattg ccctcattat cgcaatcgat attataagcc gcaggcttct taggcagacc
    21781 aggcattgtc ataatatcgc ccgtataagc taccacaaat cctgcaccgg ctgaaagcct
    21841 tacatctctt actgttatct cataattctc aggcttgcca agctttgtcg ggtcgtcaga
    21901 cagtgaatac tgcgtcttag ccacgcaaac agggatatcc ccgaatccca gctcagtgat
    21961 ctcctttata gccttttcag cctgctttgt atagatcacg ccgtttgcac ggtatatctc
    22021 ctttgcaagt ataccaagct tttccttgat agggagcttc tcgtcataaa gtggcttgaa
    22081 ctcagacggc tttgtttcga tagtcttgca aaccttttcc gcaaggttga taccgccctc
    22141 agagcctttt gcaaaaatct cagccagtga gaactcaaca cccatttcac cgcaaactct
    22201 ctcgataaca accagctcag cctcagtatc agtatggaat ctatttatag ccaccactac
    22261 aggcacaccg aacttgtgca tattctcgat atgagttttc aggtttacga tacccttttc
    22321 gagagcctca acattttcgt tagtaagctc agttttcgga acaccgccat tgtatttcaa
    22381 tgcccttaca gtagccacca gaactacgca acttggttta agaccagcgt atctgcactt
    22441 gatatcaaag aatttctcag cgccaaggtc agagccgaag ccagcctcag tgatagtgta
    22501 gtcagcaagc ttcaaagcaa gctttgttgc tcttactgag ttacagccgt gagcgatatt
    22561 agcgaatgga ccgccatgga taagagcagg attgttctca agcgtctgaa caaggttagg
    22621 cttcaaagcg tctttcagca aagcagtcat agcaccgcaa ccgcctatat ccttagcata
    22681 aacaggcttt ccgtcagttg tgtaagcaac gagtatcttc tcaattctag ccttaaggtc
    22741 agccagatcg gaagcgaggc aaagaatagc cattatctca gaagcgacag ttatctggaa
    22801 agaatcctct ctaggaatac cattcacctt tgagccaaga ccgataacaa tatctctcag
    22861 cgctctgtca ttcatgtcaa ggcagcgctt gaaaagtatc cttctctggt caatgtgaag
    22921 ctcatttccc tgctgcatat ggttgtcgat aagggcgcag agcaggttgt tagccgcagt
    22981 tatagcgtgc atatctcctg tgaagtgcaa attgatgtct tccataggaa cgacctgagc
    23041 gtatccgccg ccagccgcac cgcccttgat accaaaaaca ggaccaagtg aaggctctct
    23101 cagagcgagt atagctttct tgcctatctt gttcatagac tcagccaatc caacggaaat
    23161 agtggtcttg ccctctcctg caggcgtagg gtttatagcg gtaacaagaa taagcttacc
    23221 atctggatca tttttagttt tagcataaag cgactcagaa agcttagcct tgtagtgacc
    23281 ataaggctca tattcatctt ctgagatgcc gagcttagca gcgacctccc caattttgag
    23341 cattttcgcc tgctttgcga tttcgatatc tgacagcatt tagatcaagt cctttcggaa
    23401 aaatttttag tatatcggta aagacccata tataatatca tacgctacat attatatcac
    23461 atatccgtga tgatttcaat atttcatatg taaacaaact atataaacca acacatttcc
    23521 gaacatcgcc ccacaaaaat agccgccccg cattccgcag gacggctcaa aataactata
    23581 agaattattt ctgaatatcg ttatcgttaa atggatctcc acattccggg cagaacttag
    23641 gaggattttt cggatcttca ggctcccagc cgcacttgtc acacttgtaa agcggctctg
    23701 atgcaggctt tggctttccg cactcagcgc agaacttgcc cttgttcact gctccgcatg
    23761 agcaagtcca gcctactggc tgctcaggct ttggtgaacc gcagtttgca cagaactttc
    23821 ctgtgttctc tgtgccgcat gaacacttcc agctgtctgc tgccggcttt ggctcaggct
    23881 taggctgtcc gcagttagca cagaattttc ctgtgttttt tgttccgcaa gcacaagtcc
    23941 agccgttctc aggtgcagcc tgtgttggtg cagcctgtgt tggtgcagcc gctggtgctg
    24001 cctgctgtgg agctgcctgt gcagcctgct gattcatcat gttctgctgc atctgctgct
    24061 gatacattgt tgcgtccatt ggctgaccca tcatgccgcc tgccatgccc atgcccataa
    24121 agcctgccat aacaccgttt gcgttgcttg ccgctgcctc tcttgccttg ttgatagaca
    24181 ttctgtccat agcatagagc attgaaggat cacttccaac agtttttgcc tcctggaatt
    24241 ttgcgattct ctgctttgac tcttcgtcca cgttcagctc aatagcaagg cttgttatct
    24301 taacaccctt tgtagcccac ttttcttcaa gctctgcatt cattgcttca gcaagctcat
    24361 caggataaag accaagctga tcgtatggta ttttctgagc ggatatcctt gtaagagcct
    24421 tctggaactt aggtgtcatg tcagccttaa gctggccgag aactgccacg ccgtcagggc
    24481 tgtcaacagt aagtacctta tttacgttct gaacaacgtt ctcatagaag ttcatagggt
    24541 ttacgatctg aagttcatac tgaccatgac cctgtgcgtt aagagtgagg ttcatttcgc
    24601 cgtctctgaa aggtatcttg ccgaagccta tcttgtttcc aggcagaggt ctgaggttta
    24661 tgtagataag acgcatggtg ttcatagcct gaccacctgc tgtaaatctt ctgcctatct
    24721 ccttgataga agcgccaagg tcgccccagc cgccgccaag aagtgacgga gatacagttg
    24781 aatcatactt atactgacct gctgtttcct gtgtaccgat aacaaaatcg tgtactctgc
    24841 cattctcgat aagcaaagcc gcctgattta ccgcaacgtc aaagcgtgaa ccgtttgaga
    24901 taacgccctc tgtgccgttg ttcacagtct ttcctctctt aacaagagta gccggctggc
    24961 agagtatctt agttgacata tcctcacatc tgaagtactc tactacctga tcggcaaatg
    25021 ttgagccgac tgctgatgta aacatcttaa taagtcccat agttaaattc ctttcgttgt
    25081 ctgtattatt tcaccccaag gggttttaaa cttgtctatc tcagcatgga tatcctgctg
    25141 actttccatg tgcgctctac agagctgtgt atctcagaac cgcagtaagg acaaaccgag
    25201 cctgcggtgt ccacaggtgc tccgcagaac tgacatataa ggctggcgtt ttcgccctct
    25261 atccccattg aaagatagta tgaataatcc gcctcataaa tgtgctgtgc ctttatcccg
    25321 ttgagcttgt attcaagaga tacctgaaac tttgccgttg cagcgtcatg atctctatga
    25381 taagacgcca cctgcgactt gtggatatgt atatcgtcat aatgctttgg cgtaaccgtt
    25441 gacaagttgt cttttgccat cgtggctgct ctctgcttga acgcttcggt acagtattca
    25501 agctgattct cgcttgcttc tccgtttata acaagaaaat atttcatcac agtgttttcc
    25561 actatgctcc ttgctatatt ttcatcatag tcaggaaaat ctctctttat gttgttcacc
    25621 acattaggat caacataagt aaactgcatg gcgttgttct gtctttcctc agttgcgtca
    25681 tttatggtcc ttagcaggtc gttggcacgg ctcagttgtc tgccaatggc gttcgatacc
    25741 gcttttttca cagaatatat caccgctata ccaccgccta tggcgaccac tataactatt
    25801 atttttatta taagcgccgt tgatatttca ggcatttagc tgataccccc tactagaata
    25861 ttcccattat ctgaagtata gctccgataa tatatccgcc cacgcctcca agaagcgagt
    25921 aagccacaag ctttcccttg cttataggca ggtttccgat aaatttacct gtctgaccat
    25981 tcattgcaaa gaaataaggc ttatcctgat aggttgtata caaaagccat gttggaagca
    26041 tagcatattt aacgtccttt atctcggtgt gcttttcata tgactgaacg tttacactgt
    26101 tgtagttaca agtatttcta agttcgtcac gagttgtgtt ctcgatacgt tctttagccc
    26161 taggcaggca cttatcctta tcctcgtcat atctctctgc aagatatccc gaaagataac
    26221 ctgggttgaa cgctgtcatt tccgagtaat caaaaggctc gatagagtcc atagcgtcat
    26281 cgtcagtctt tgaagaagca tccacaggaa cagctttgaa atcgagggtt ccgtttctgt
    26341 aaactgaata atactttttc tcagtgtatc tgtagtttcc ggaagtccat gttcttgtgt
    26401 ttataccctc tgcagtaata gagccttcgc agtttcctga atagagccaa aacgggatat
    26461 aaacgccctg catcttgtct actaccctat cgcagctgaa atctttcggt gtaagaggct
    26521 tcttgctgaa tttttggaaa gcgtccatta cctgattttt cggcactttg aaaggtataa
    26581 catagtcagg agagaaattg tctattatct gctctcccat tattaccgca tttccgcagt
    26641 aaacgcaaac cgttgctgca gttgagcgat cggtgattat ctccgctccg cagtgtgagc
    26701 aggtatattt tacaaggtca tttcctacag ttccgtcatt tgacatactg tattcgtcct
    26761 tatcgacctc ttcatgcttt tcaacgttat ccttgttgag ttcctccgcc tttttagcgt
    26821 caccgcctgc ttcttcaagg tccttcaaag taaactgtcc gtcgcagtat tcacatgccc
    26881 agcagttttt gtcagcactc cagaaaagct ctgctccgca gttagggcat ttataagaaa
    26941 tattaacagc cattttccgt accccctatc aatcgtcttt tatcttagtc ttatcagtgg
    27001 tggtacgcag ataggtgtcg ttacgcccat tgagtttaag attactcttt atgtatttat
    27061 ctgccttggt agctttctgt tttgtgcggc tcattgaaac aagcacaaag cctgttataa
    27121 gtcctcccgg tatagcaaag cacatatttg ttcccacgct cacactgctg tcggcatatg
    27181 ccatgacagg ggtgaaaaac aaattgcagg aaataagtgc aaagcctaat gtgcggtata
    27241 gctttttcaa cattttttct cctcccaact ccatattttc acatatattt atgataacag
    27301 aaaacgcttt ttatgtcaag aaatatgtca tacaacgttc aaaaaaaatt cttttttgtc
    27361 catttaaaca tatttttata cccctcattg gtgtttttaa cgattgacag ccgttctgtc
    27421 aggaatcagc gttattcagc ggttttctgc tcgtcaaggt caagagatat cagagggttt
    27481 gcgtcattgg ccaccttagg cagaacaccg tcccacttat cgatggtctg atactttata
    27541 agctcgtttg aaagagattc agagatagtc ttgttggctt ctgcctgagc ttcagccttg
    27601 gtctttatgg aagttgcctc agcgtctgcg gcaataacct ttttcttagc ctccgcctca
    27661 gctacaacta tagcctgctc ctgctcggtc tttgttttca aaagattctg ctctgctacc
    27721 tgctttgcct ctatggcttc attaaattca gatgagaaat caaaattcac aatgttgaat
    27781 ttctctatct cgataccgta ttcatccacc tttgaaacga gctgtgcctt tatttcctcg
    27841 cccacctgcg ctctgtcagt gataagctgt tcagccgtat attttgcaca taccgacttc
    27901 atacattcct gcactgttgg cataaggata acgttctcgt aatctgagcc gatattcttg
    27961 aaaatgcttg cagaagaagc cgactttatt cggaagttca ccgctatggt agagttcaca
    28021 gtctgcaagt cctttgaaac tgcgtcagcg tcagcctgat aaacttgtat cttgtttgac
    28081 actgttgtta cacgctgtgc aaaaggcact ttcagatgca atccttcgtt gagtgcagtg
    28141 tctgatacct tgccgaaggt cgtcacaacg cccgtacagc ctgccggaac tatggacacg
    28201 gaggatacga ccactattat agccgcaact gccgccgcac aaatgccaac gatctttcct
    28261 gtttttcttg ttgaaatgtc aatgaccttt tctttgctca tgattttttc ctttcatata
    28321 aaacaaagtc tttcgacttg ccgtctaacc aaaggctttc tgtcctttgg tttaatatga
    28381 ttatatcata agccttacaa attagcaaag tccgacaaag ttcggggaag tcagcggaag
    28441 tcagcagtga acagattttt tgatttagct cgctgtatct cttgcaatct cacatattct
    28501 cgttcgttca aatccgctcg ttatttatgg gagaaaaaat ttggcacagg tcaatagtca
    28561 tatttcaata tatgtaaaag ctaatatgtt tatatcattg cctgcaaaaa agcataaaaa
    28621 aaatcggact cattttactg cgtccgaatt ttttatcaat gtattgatga acaagcggga
    28681 ggcccaaggt cgcccctaca gataggtttg ttagctcttt ttctttgcac tggattttac
    28741 cgctgtgaaa ctccacacaa gtccagccgc gatccagaag tatggtgatg aggtaacaga
    28801 acttatgttg aaaaacgcct gtatcagata gcaaagaacc gccgctgaca ctgcacagcc
    28861 tgtccagccg tttttgtctt tcataaagcc tgctacgagc ttgcacattt ttactatagt
    28921 cataataagg aatactattg taagtgcgaa aatgatgagt cctcttgact gcaaaaggtc
    28981 gatatactca ttataagagc ggtctattat agcacccgac tcgaatatcg ttccccagtt
    29041 gtctggaccg gttccccaga taaggttttg ctcggcagta tacattccgt cgtcccacag
    29101 ataaggatat atccactgtt cggtatcatc gcctctgcct gtgcctgtga tagaaagtct
    29161 gtcgaagctg tcatggaaga ttattttctc gtccataagt ctgaactcgt ttgaggcgaa
    29221 aagtccagca aagatccctc ctgcgactat aaaagccaca aggcaggcta taaaaggctt
    29281 cttatccttt gcaaagcccg actttaccgc ggcaataata agagatataa cataaaccgc
    29341 accaagtccc aagatagcag ggacaacgca ggcgaggata tcagccgctg tcataagggc
    29401 aagggagata gcatagaata tctttcttgc ctttttatca tcatatagca taccattaag
    29461 cgcaaaagca gatatcactg taagcactgc cgccaatgca aaaggcgtac acaaaaagcc
    29521 tgttgcgata tattcctttg tataggtcat accgcctgat gagaagcccg agaatatctc
    29581 tttgaaatag tttggaacta tttttgcggt agcaggtata gcctgcaaaa ttcccacgat
    29641 agcttcaaat gcgcctattc ctacgataaa gtctgaaagc tttatcctgc agtcgtcagc
    29701 cgtaactgtc attcctgcgg caaaaagtcc ccaatagcca agtatggtga gcataccctc
    29761 tgagcggtcg agatagccgt agaaagatgt gaagatatcc gctgaaagca agcaagatac
    29821 caccgacaga agcaccaccg caagtggtac gataaggctc ttgttgtctt taagctccac
    29881 ctgctttttc atgttggcaa taaggaaaat gataaatccc acaaagccga ccgcaagcac
    29941 agcggcagat ataaagaata taaatttttc gtttagataa agagtacggt tgacgctttc
    30001 gtccataccg tatttgatat tctgtgtgaa ataatacggt attgtgataa cacacagaac
    30061 aagcacagca ataatgctgt aaactccgca cagcttttgt gcagactcgg cagacatatt
    30121 cagaataaaa tccgactttt ccgaactgcc gaaaaggtgc tttttacctt gattggtagt
    30181 gacctctttt ttctcttttg cttttgacat aaaaaatccc ccaaaaagca tagatatttt
    30241 attattttat gattgaaaat taaaaatcat cgccgcaggc gataccttaa ttcctcactc
    30301 ctcactcctc attcctcatt aaaaacagag cggggaaata aaaaagcagg caaaccctaa
    30361 agtctgcccg ctgtttacac aatttacttt gcgtattcga tagctctgct ctcacggata
    30421 agattgacct tgatctgtcc cggataatcc atttcagttt cgatcctctt agcgatctca
    30481 cgagcaacga gcaccatttt ttcatcgttg atcttctcag gctgtaccat gatacgaacc
    30541 tcacggcctg cctgtatagc aaaggatttc tcaacgccct cataggacgt gcagatctcc
    30601 tcaagcttct ggagcctctt gatatagttc tcatagttct cgcttcttgc tcccggtcta
    30661 gcggcgctta ttgcgtctgc cgcctgaaca agtgccgcaa caacagttct tgtttcaacg
    30721 tcgccatggt gagcctcgat agcatgaata acttcagggc tttccttgta cttcttacat
    30781 acgtcaacac ctatctgaac gtgcgagcct tctatctcat agctgagagc ttttcctata
    30841 tcgtggagca gtcctgctct tcttgccaga tttgcgtcaa cgccaagctc tgacgccatc
    30901 attcctgcaa ggtgagcgac ttcaatagag tggttaagca cgttctgtct atagctcgtt
    30961 ctatagcgca gacgtccaag aagcttaaca agctcgtggt tcacaccgtg aacattggtt
    31021 tcgagaatag ctctctcgcc ctcctgcttg atcttcagct cgacttcacg cttagccttt
    31081 tctaccattt cctcaatgcg tgtagggtgt atacgtccgt cctggataag cttttcaagt
    31141 gcgatccttg cgacctctct tctcacctga tcaaagcacg agatggtgat agcctcagga
    31201 gtatcgtcaa tgataagatc aacgcctgtg agagtttcga tagttctgat atttcttccc
    31261 tcacgtccga ttatcctgcc cttcatctca tcattaggca atgcaacgac tgacactgct
    31321 gattcagaca cctgatcggc ggcacacctt gagatagcaa gcgaaatata ctgtctggcc
    31381 ttagcctcac agtcatcctt cacctgattt tcaaagtttg tgaccctcac agccttttca
    31441 tgcaccagat cctcgtccag ctttgaaagc agataatcct tagcctgatc aacagtgaac
    31501 tgagatatct tttcaagcat atcgagctgt gacttcttga tcctgtcagc ctcagctacc
    31561 ttttcgtcag catttttcag cttgttctgg agagtttctt cctttttctc caaattatcg
    31621 agctttctgt cgatagactc ctctttctgc tgaacacgtc tttcctgtct ggaaacttcg
    31681 atccttctct ctttaagctc tttttccgtt tcctggcgtg acttgtggat ctcgtctttc
    31741 gcctcgatca atgcctcttt tttcagagtt tcagcgtttt tctttgcgtc gtcaacgata
    31801 cgctccgctt ccttttccgc agagcctaca gcagcttcgg ctgtcttttt gcgatagctt
    31861 ataccctgtt tgaagcagat aaatccgctc acaccggcac caagaacaag agcaactaca
    31921 catagtagaa tagcaatacc tggttccatg tatagctttc ctccttctta gaaagtatgg
    31981 atatccatat tttgtgcgta aaaaacgcat gaaaaaagat atccacctga tattaaattg
    32041 taaagataca aaattgcaaa atttatttta taaaaaacat cagaataata caaaaaacta
    32101 tacttcatct ttcagttata taaaaatnnn nnnnnnntta ttgtataata aaacttctct
    32161 gttgtcaaga atttttttag ttaaatccac aaaattatat attttctaac aaaccccttg
    32221 acaagcgggt gaagaggtgt taaaatgtta gtcgaagcaa aagataaagt cttttgcagt
    32281 tatacttgaa agctttgacg gggaagcccc tcggcgaaag gtcttttcag agagcgccgc
    32341 acgtttttat acgagctgtg agcggtgcag gactaggcag agtgaggata ccgcccctga
    32401 gtgcgtttaa tacacgccgc aggctcagcg ttaaaggagc agctaatgag ttgtgcagga
    32461 aactgcgcat gaagttgggt ggaaccacga tatcatcgtc ccataggata tttgtatcct
    32521 atgggacttt tttgtcatgt aagagggtga tgatactatg aaagacagga aagccatgaa
    32581 aacaaggcgt atccttgcga tcatttgcta taccattggg ttttgcgctt atggcgcagc
    32641 gggatatgtt tgctggttat catggaacgg gactatatcc gacaagcaag ggtttctcac
    32701 cttcctgccc atatggatag tatcatactg gttcaacaca tttttctctc agcttgtttg
    32761 cagggtgaaa aaagacggct ccaggcaatg ggctatcaaa aaaagggcaa ggcacgtttt
    32821 gagcgacatt tcaacggtgc tgagcgtagt gcttctttgc ttctggatat atgtgtatgt
    32881 ttcgcagtat acagaactgc tgaacttcaa ataatatgcc tgtaggggcg acctcaagtc
    32941 gcccacaaaa aaacgtgtga tatatgacgc attacaaaaa gaaacaacgt gtttatgcac
    33001 gttataataa agaattacaa tattatataa atcggaggaa tcaaaaatga tcaagctgaa
    33061 attaaaagac ggctctgtcc gtgagatcga aaaggcaatg cctgcaagcg agataatcaa
    33121 ggatatcggc atgggacttt acaaggctgc ctgctgcgta aagctggacg gcgaagtttt
    33181 cgacatgaga actgtcgttg accatgactg ccattttgag gtcatgactt ttgactcaaa
    33241 ggctggtaag gaaactttct ggcacacagc ttctcacctt atggcacagg ctgtaaaaaa
    33301 tctttatcct gaggcaaagc ttgcaagagg ccctgctaca gacacaggct tctactatga
    33361 ttttgaggta gaaaagcctt ttacgcctgc tgaccttgaa aagatacagg ctgaaatgaa
    33421 gaagcttgcg aaagagggct atgctttgga gcgtttcgag ctttctgctg acgaggctat
    33481 aaagctcatg gaggaaagac acgaagatta caaggatgac gataagggcg aaaagctttc
    33541 attctacaag cagggtgatt ttgttgacct ttgcgcaggt ccgcacataa tgagcgttgc
    33601 acctatcaag gctgttaagc ttctttcatg cacaggtgca tactggggca aggcagagga
    33661 cggcaagcag ctctcaagaa tttacggaac tgcattccct aaggcttcaa tgctcgatga
    33721 gttccttaaa cagcaggagg aagctaagct tcgtgaccac aacaagatag gcagagagct
    33781 tgaatatttc acaactgttg attgtatcgg tcagggactt cctgttatgc ttccaaaggg
    33841 cgcaagagtt atccagcttc ttcagcgttg ggtagaagat gtggagcagg caaagggctg
    33901 tctgcttaca aagactccgc ttttcgcaaa gagagatctt tataagatat caggtcactg
    33961 ggatcactat cttgacggaa tgttcattct tggcgaccca tatgacgagg aaaaagagtg
    34021 cttcgcactt cgtccgatga cttgtccgtt ccagtaccag gtatttctta acagacagcg
    34081 ttcatacaga gagctgccaa tgagattgac agagacttct acgctgttca gaaacgagga
    34141 cagcggtgag atgcacggtc ttatcagagt aagacagttc actatctctg agggtcacta
    34201 cattctcaga cctgaccagc ttgaagagga gttcaagggc tgtttggagc ttgctaagta
    34261 tttccttgac actgttggtc tgcttgatga ctgcacattc agattctcac agtgggatcc
    34321 tgctaaccct aagaacaagt acgagggcac ggctgagcag tgggagcacg cacaggctgc
    34381 aatgaagact attcttgacg accttggcgt tgattacgat atcggtatcg acgaggctgc
    34441 gttctatggt ccaaagctcg acattcagta caagaacgtt tacggcaagg aagacacgct
    34501 tgttactatc cagatagata tgcttttggc tgaaagattt ggtatgtact atgttgacaa
    34561 ggacggtcag aagaagcttc cttacatcat tcacagaact tcacttggct gctttgaaag
    34621 aacacttgct tatatgcttg aacatttcaa cggcgttctt cctctgtggc tcgctcctga
    34681 gcaggtaaga cttcttccta tcaaggaggg tcatgctgaa tacgctcaga agatagccga
    34741 cagaatgact gctatgggca tgagagtaac tgttgacaac agagatgaga acattggtac
    34801 taaaatcaag gctgcaagac ttgagagaat accttatatg ctcatcattg gtgacaacga
    34861 ggtaaacagc aacactgtta ctgttcgttc aagaaaagag ggcgagcttc ctgcaatgcc
    34921 tgttgacgag cttatcgcta agcttgatga ggagataaag actaaggcga agtaattggt
    34981 agcaaaacat cgaaaggtgc gtaaaaacgg cgtttgtggc aacagacggg acgtcgaggg
    35041 cgccgtcccc tacaaattaa tgtacgggcg aaaactgttg tgagccttgc caaacaacaa
    35101 ttttcgttcg cccgttggct tttgctgtca atgtgtttta tacagacgtt ttatgcgata
    35161 ccgcacaggg ggagtttctc ttgtgcggcg ttgccgttta taaaatatgg ggcggacagg
    35221 ataaaatccg cttcgggaaa gaggtaatag atgaaatcag atatgatctg gacgcttgtg
    35281 gcatttgcat tctatttggc aggaatgctt ctcataggtg ctgcatatat gaagaaaaca
    35341 aaaagctccg aggacttttt ccttggtggc agaggactta acggctgggt agcggcactt
    35401 tctgctcagg cttctgatat gtcaggctgg atgctcatgg gtctgcctgg cgctgtttac
    35461 gcattcggaa cggggcagat atggatagct gtaggtctgc ttatcggcac tatacttaac
    35521 tgggcgatag tttcgggcag actgagaaga tacactataa gagcgaacaa ctcaatgaca
    35581 cttcctaagt atcttgaaaa cagatttgag gacaagcaca agataatgct tttggtatcg
    35641 tcggtggcaa tcgtcatatt cttccttgtt tacactgcgt ctgctctttc ggcaggcgga
    35701 aagcttttca actctatatt cggtatcgac tacaagatat gtcttactat cggtgcggta
    35761 gtaattttgg gatacacttt catgggtgga tttatggcag tatgtgtaac ggactttgtt
    35821 cagggtctgc tcatgctggc aggagttgtg actataccta tcatcgcatt tttccttgtt
    35881 ggagcaggaa acgttatgga caacatcact gcaagcggtg ttgaggcaca tcactatctt
    35941 aacatattca atgatgaaaa gggtcagacc ctttcagcca tcgacataat ctctaagctc
    36001 ggctgggcac ttggttattt cggtatgccg cacattctgg taagatttat ggctataagc
    36061 tctgaaaaag aggtaaaaaa gtcaagagtt ataggctgtg tatggtgtgc tatatcactt
    36121 tgcttcgctt gctttatagc tatcgtgggc agggcttatc ttgtgcctgc acttgcaaac
    36181 aacaagtctg agaatgtttt catcgagatg atacagaagg tattcaactc cgatattggt
    36241 ctgccattcg tcggcggaat tttcctttgc ggaatacttg ctgccataat gtcaacggct
    36301 gattcgcagc tgcttgtaac tgcctcatcg gtatcggagg atatttacca ctcatgtatt
    36361 gcacctaagg cggagagcaa gaaggtcctg cttgtgagca ggataacagt tcttgctgta
    36421 gcggtagtgg catacataat agcacttgac cctaaaagca gtataatggg tcttgtttca
    36481 aacgcatggg caggtcttgg agcttcgttt ggtccgataa tacttatgtc cctttattgg
    36541 aggagaacaa accttgcagg tgcggcggca ggtgttgcca gcggtgcgtt atcggtaata
    36601 atatgggatt acatacctct tgcaggtgga cagactttag gtgacaagac ggggctttac
    36661 tcattggtag taggttttgc ggtaagtctt atacttatta ttatagtgag cctttgcaca
    36721 aagaaaccgt ccgaggaaat gctgaaggca tttgatgatg tcaagaacgg tgtgaactgc
    36781 ggttgtgatg aaaaagcata acaaagtaaa aggtccatgg gtacgcccac ggaccttttg
    36841 tattttatct tttagctatc atacttttcc ttattttcgc atctgaatta cgtaatcacc
    36901 gctttattgc ctttatcctc atatttctga gcgtaaagac caataacaac tcccgtgaaa
    36961 ttacctgcaa gctcagtaga caaaaacttc gtttgagctc cgcccaagtc tatatctccc
    37021 gaaagcgttt tcgccgagaa attgtatgta aagttggaag cattacagca aagcacagcc
    37081 gagctttctc cctgtggcaa tgctatgaca tgatcctcat ggcgaatatc gcctatgcaa
    37141 agtcttcgga atagctcgac gcctttatct gtgcgtctta acgctatctc ataatgctga
    37201 tctggggtca tgtagaacac caatcctgct tcgccctctg ttatctcgac tttgcactcc
    37261 acgtataaat tcatttcttt ctgtcttatg cagataaaag caggcgagcc gtctgcctca
    37321 aagatcgtat gctcgtttgg cgtaagctca aagcgttttt tatcgagctt ataaaggttc
    37381 atatcggggt tttgcataaa gcaccactca cgtccgactt tggtattctc gaaagtgtat
    37441 tctttccaaa actcctgcct gatatccccg agtctgtcgg tttcaacttc cttgcgagtt
    37501 gtgccattat ctcccattgt gaaccagccg ttctcgtcga aagtcactgg tacgagatag
    37561 acttctctgc ctgtaatgtg gtgcatcacc cacatattta tcattctgaa cgcaaggtgg
    37621 acgcaccaga agttgccgtt ttcgtcctcc acaaggtcac catgaccgca cccttgtatc
    37681 tcgtatccac caagatttct gtttgttaga actgggttgt gaggataatt ctcaaaagga
    37741 ccataagggg tcttaccttt ggcatagact atcatatgac cgtattccgt gccgccctca
    37801 gccgccataa ggtagtattc gccctttatt ttgtagaggt gtgggctttc aaggaaacgt
    37861 ccgcctgcac ctgtccatat ggtcttgctt ggcgtgagct ttttgcctgt ttcgatatct
    37921 atttcgcact gtgtaacgcc atggttgccc tcatcgtctt caccattgga cataaagtag
    37981 gctttgccat tctcaaaata cagtgacggg tcgataccgc cctgctgaac ataaacaggc
    38041 tcagaccatt caccgtggat atcgtcagta tagacataga agtttccgcc atttgtgacg
    38101 ttagtcgtta ccatgtaaaa tctgccgtta ttgtatctga tagttggggc atagatgccg
    38161 ccccaagagc ctgcgccctc caatgggagc tgactttttc ttgtgagaac atgacctatc
    38221 tgcttccagt tcacaaggtc gtcgctttca tatagaggaa cgcccggaaa aaactgaaaa
    38281 gagctgcaaa caagataata tttgccctca gccttacaga tgctagggtc gggataaaag
    38341 cctgatatga ccggatttat gtacttcata ttgtgacctc cgtttatatt ttacagatat
    38401 tataatctat ttcagatggc ttttcaatat cttctcgtgc aggatcggtg aatttttgtg
    38461 caaatgaaaa acaaatttca gtttacgatc tttgcgtttg ggtaaagcaa tcaattgata
    38521 cggatatcta agtgggatag tagctttgac gggcgaacga tattcgcccc tacagttttt
    38581 tgcggggata tatgttaagt taaacgtttt tgatagcgtg ttaagtataa aagttacaaa
    38641 ttgtaatttt tgctccgaca tttgttcgta tggcatataa cagtctatat tctggggata
    38701 tatttgaata tagaacttgt gttccctgtg aaaacaaaca aatattttag tcatttcggc
    38761 aaaaagcgat aaaatctgtt gccaaattgt aacctttatg atattatgaa gttgatatac
    38821 gaaaacgtat aagataattc tgtcagttag ggggcgtttg gaatggaaaa ggggctgaaa
    38881 aaagtttttt cggctatggg cgcagtattt tgtgctgtta tgatgatcat gctttgtcag
    38941 gtgacaaatg cggtgagcta cagcgaacag accgtcaccg tggactatga ttatcaggtc
    39001 atagactctt ttgagggcgt tgacgctaaa tacgttctcg gctattccga caccggttat
    39061 tactgctgtg caggatatgt ttcacgtttt tatgaagaaa agttcggcgt tacagtatac
    39121 aacataaaca tggtggacga caagcctagc gtgtactgct acggcaaaaa ggcggagctt
    39181 cgtgaagtga aaactccgca ggccggggac ataatgcagg acaaggatta ctctcatgtt
    39241 gccattgtaa aggactgtca gggaacgacg gccacgctta tcgagcagaa ctacaaatgg
    39301 acttggaacg acactgttta cactgtgaaa aacagaaagg tacttactaa caatcactat
    39361 ttctacagac tttacataaa cggcgtcgca caaagccttg acatcgacac gacaagacct
    39421 gttatcgcaa acgcaggctg tgagaacatc acgggaaaag gctacactgt taaagcggat
    39481 atttctgaca acagggctgt ggcttcggta aaggtatcca cttattttga gggcaacaag
    39541 gcaaagacta taagccgtgt ttacacatcg gcggtgaact cgctttcaca ctcagtaaag
    39601 gtaactgacc ttgggggcaa ggacggatat tatattacaa ccataacggc tactgacgag
    39661 gccgggaaca gctctgagca ggtcataaag acttatgttg acacaactgc gccgaccata
    39721 tcgggggcaa aggtggagaa catcacagca aagggctttg atgtttcttg caatgtgagc
    39781 gacaagagcg gagtttcaaa ggtggtcttc cctgcttacc ctacaaaaaa tggtgaaagt
    39841 gcaaagaaat acgcaagcgg aaagctttcg ggtggcaagg cttcggctta tgtttctgcc
    39901 gaggactatt cagtaaaaag cggtgaattt accgttaaga taacggctta tgacaaatac
    39961 ggaaacagct cagcaaaaac tataaaggct agcataaagc ctgcggcttc ggtaacattg
    40021 gataaggctt cactgactct tgacaatggc aaaagcagtg ttatttctgc aaaaatgggt
    40081 ggcggaagcg gtctgacgga ctttgtttcc tggaagtctt ctaacagcaa aattgcttct
    40141 gtgtcaaacg gaaaggtcac tgcaaaaggg gttggcagag caactatcac ggcatacaca
    40201 acaggaggaa aaaatgttaa atgtaccgtc actgtaaagg gtaaaatcag tgacagcagt
    40261 atttcggcta taaagacgca aagctacaca gggaaggcgg tttctcctgt gcctgcagtg
    40321 acatatggtg gcaaaaagct tgtaaagaat actgactaca ctgtgagcta ctcaaagaac
    40381 acagccatag gtcaggcaag cgtaaaaata acaggcaagg gtttatacaa aggcacaaaa
    40441 actgtgaact tcaacattcg tcctgccaca gtgacgaagc taaaagtttc ttccaccggt
    40501 gaaaagagcg ttaagctttc ttggaaaaag attacaggtg ctgacagcta cgctatatac
    40561 agatatgaca acacaagcaa gaaatggcat agaataaaaa ctgtaaaggc tgtgagcttc
    40621 actgacagcg gacttgctcg tgcaaagggg tacagctaca aggtaaaggc tgtgaaaaaa
    40681 tctggcggaa aagaatacat ttcggctagc tactcaaagg ctgtagaggc tgtcacaaag
    40741 cctgcaaagg cttcatgcac ggctagatca gcaggcagcg gcagcattga ggtatcatgg
    40801 aaagctgttg ggggtgcaag cggatatgag atatactcct cttcaaatgg cagtgattat
    40861 gtgaagtcag caaaggtggg tggctctcaa aagagtgcta tcgtatcagg atttgagcct
    40921 tactcactta gaatggtaaa ggtaagggct tacaaaactg taaacggaaa gacttcctac
    40981 ggggcttttt cgcaggcggt tatggttatt gttaggtagg ctggatacag aaacatatga
    41041 tatataataa gcaagcgata ggtgatatgc gggcgaccaa aggtcgcccc tacgatagtt
    41101 caagcattgt aggggacggc gccctcgacg tcccgatttc ggaacgttac catttgtaag
    41161 attgcaaaaa acttgtgtaa tttcgccata tatcatcagt cgccactacg atacatgact
    41221 ggtacgctac acaattgagg taaaccaaac gagcgaatga tgttcgcccc tacatttttt
    41281 attgctacac aaaaaaggac agcactttgg aaaaatgctg tcctttttct atattcatat
    41341 aggtctatct ttccattttc cagaactcta cagagtatgg cttaagctcg ctgccgccac
    41401 caaaatcatg gatagtgcct gtgagaagct cctctgcctg accgcagcct gcgtcaaagt
    41461 gagctgtgaa aggctgatcg tcagcgttta ttgcaacaag gacacgctca ccctcgaact
    41521 cacgctcgat tatacactgc ttattggtaa gaacaggtat tttgatagag ccatagcaga
    41581 gagccttgct gtttttgtgt gcctctgcca tttttgagat aagctctgta aggtcgttcc
    41641 actcaggctt ttcaaagctt acacggagcg cagggtcgcc ctcgctctta tttgcctttg
    41701 ttccccactc tgagccgtaa tatacacatg ggataccagg cataccaaag agcattccat
    41761 agataagtgg caaatgacgc tcatttgtga gctggcttgc tattcttgat acgtcatggt
    41821 tatcaacaaa acacagcagg tgcatacctc tgtatctgca ccagttctca ggtccgaact
    41881 gatttttcag tgaatggcat atctcgaaca tattcatgca gttgaagctt gaataaagtc
    41941 ccttatagca ctcgtagttc gtgcaggaat ggagcatttc aggatttacc cactgagcgt
    42001 aatcaccatg cagaagctct ccgataagaa cgaaatcctg cttgagccaa tcgcaatgct
    42061 gtctgaggcg tttcaagaag ttcttatcaa ggcaatatgc aacgtcaagg cggagtccgt
    42121 ctatatcaaa ctccttgacc caatttgtta tgcattccaa gatatgatcc actacctgcg
    42181 gattgttaag gtttagctta acaaggtcaa agtgaccctc ccagccctca taccaaagtc
    42241 cgtcattata gttgctgttg ccgccaaaat tgatatggaa ccaatcttta tatggtgagt
    42301 tctcacggtt tttcagaacg tcctgaaaag cccagaagcc tctgccgacg tgattgaaaa
    42361 cgccgtccaa aactaccttt atgccgtttt cgtgaagctc tttgcagacc tttgcaaagt
    42421 cctcatttgt gccaaggcgg caatctatct tgcggtaatc tctggtatta tagccgtgag
    42481 tgtcggaatc gaacaccggt gaaaaataga tagcgttggc gccaagcttt ttgatatgag
    42541 gtatccaatc tattaccttt aatatcctgc tttcctcttt gccgtcattc tcaaatggcg
    42601 caccgcagaa tccaagagga tagatctgat aaaatacact ttcgtatgcc cacataatat
    42661 tatcttcctt tctgatacta atagaaaatg ttgttcgcag atgaaatatg gtttgctgag
    42721 aaaaaagcgg gcgaaagctg ttcgccccta caggggtggc atgacgtttc tagtccttaa
    42781 atttctcagc ttatttatat tttatgagat atctgcgaaa ctatagttaa actgtacaaa
    42841 atacaacact attattatat caaatcccaa catatttctc aatactcatt tttcattaat
    42901 ataatccaca cttttgtgca ttttgtacaa ctattgacaa atcaatcgtg agatgatatt
    42961 atatttataa caaatgtcaa gggttgattt ttggtaaagg cagggataaa aacatgaaga
    43021 agatacttgt gcttttcaca ggcggaacta tcgggagcat tactactgac ggcataatag
    43081 atgttgagcg tgagggaaaa tacgctgttg tggaggctta caagagtgca tacggcgatg
    43141 atgtggaatt tgaatgtcga caggtactta atatactcag cgaaaacatg gggttttcgg
    43201 ggtgggagaa aatgatacac gagataaaca gcattgacac ggctttatac tcgggagtta
    43261 tcctcaccca cggaagcgac acgctggctt acacttctgc tctgnnnnnn nnnngggtca
    43321 tgcttcctac cagggtgata cctgcagaca cctgtcggga caggtttggg gtatacggtt
    43381 atgacggctt caatgatttt tcggttttca gcggagtgag cgagaatatg ctgtcacgcc
    43441 ctagggaacg gattttccat aatgatgtca aatttgggaa gcgtgttctt tttatagagg
    43501 gttatcctgc aatggacttc gggtgcttta tgccaaacgg ggacacggta gcggtgcttt
    43561 actctcctta tcacagtgcg acggcttgca ctgacgtttc agaagggaaa agctatgcac
    43621 tgacggaatt tatcaagcgg tgcataacaa agaacattat ggtgtatatt tgcggtctga
    43681 aaagaaaagt gcctatatac tccactgtgg cggagataat aaaggcaggc agcataccaa
    43741 tatacaccac ctcagcggtg gcggcttata tgaagcttct gcttgcttac aatcaggagg
    43801 aaatgtctgt gaaagaggtc atggggaaag accttttctt tgaggaggta aaattctcat
    43861 aaacattgtt tcagttattg acaaaatgct gagcttgtga taaaatcaaa gtatattaaa
    43921 atagtatatt tagaaaaagt acaacggagg taataaaaat ggcagacaca aaaatttcag
    43981 actcagttat ttatattggt gcagacgatc atgacattga cctttttgag agccaatatg
    44041 ttgtgccaaa cggcgtttcg tacaactctt acgttatcat tgacgagaag atagcggtaa
    44101 tggacactgc tgaccctagg gtaacggacg tttggtttga caatcttgaa aaggctttgg
    44161 gcggcagaaa gcctgattat cttgttgttc agcacatgga gcctgaccat gcaggaaaca
    44221 tcaaaaagct tgctgagaaa tatcctgaaa tgaagatagt gggaaacgca aagactttcc
    44281 ctatgatgaa gcagtttttc accattgacg gacttgacga taggtctgtt gtggtaaaag
    44341 agggggacac gctttctctt ggaagccaca cacttcagtt cgtaatggcg cctatggtac
    44401 actggcctga ggtaatggtg acttacgagc agaccgaaaa gcttttgttc tctgctgacg
    44461 gtttcggaaa gttcggcgca cttgatgttg aggaggactg ggcttgcgag gctagaagat
    44521 actatttcaa cattgtgggc aaatacggcg cacaggtaca gtcgcttctt aaaaaggcgt
    44581 cggcactgga cataaagatg atactgcctt tacatggtcc tatactcaaa gaagatcttg
    44641 gttactatct tggactttac aacacatggt caagctatca gcctgagagt aagggcgtat
    44701 tcgtagcgta tgcgtcgata cacggaaaca ctgcaaaggc ggctgagaag ttcggcgaga
    44761 tgcttaaaga aaagggtgct gagaaggtag ttatcagcga tctttcaaga agtgacatgg
    44821 cagaaaatat cgaggacgct ttcagatatg acagaatcgt acttatggca agcagttatg
    44881 acggtggtgt gttccctgtt atggaggact tccttatgca cctgaagtca aagaactatc
    44941 agaacagaaa gataggtctt gtagagaacg gttcatgggc tccgactgcg gcaagagtta
    45001 tgaagggcta tgtggaaaac ttcaagaatg ttaccattgc tgagcctgtt gtgactatcc
    45061 gctctacgct taatgaggct tcggaaaagg ctttgggcga gttggctgag gtcatggcta
    45121 agggtgaata aatttcgctc agtcagataa atcagacaca aaaaaatgcg ggcggccaga
    45181 ggtcgcccac acagatatga aaaaggcagg acgttttgtc ctgccttttg tgttatatca
    45241 cattgccgtc tgtggtgatt attcttactg ccacggggat atttttgccg ctgtcggtga
    45301 ctgcttttct tactgcgtgt tctattccgc cacagcatgg aacttccatg cgggctactg
    45361 ttatgctgcg gatattgttt cttttcacta tctcgccaag cttttcggaa tagtctgtgg
    45421 cgtcaagctt cggacagcct atgacggtta ttttgcctgt catgaaatcg gaatggaagc
    45481 ttccgcaggc ataggctgtg caatccgcag cgacaaacag gtctgcgccg tcaaagtatg
    45541 gagcgttcac aggtacaagc tttatttgca caggccactg cataagctgt gagggagtct
    45601 ttgatgttgg gatatcagtt ggagctggct ttttggcaaa ggttttcatc attgagcctg
    45661 gacagccatg gaaaaccggt ttgccaagac gttttttgtt ttcttccact gccttttcgt
    45721 cataggcggc agcttctcgc tcaacaaagg atatggcgtt cgttgggcag gctggaagac
    45781 aatctccaag accatcgcaa taatcgtcac gcataagctt tgctttgccg ttcaccatgc
    45841 ctattgcacc ttcgtggcag gctttagcac aagcaccaca gccgttgcac ttttcctcgt
    45901 ctatttgtat tactcgtctt atcatatggt tacctccgta tttttattga tatctttcgt
    45961 tttggcataa agctcttgac tttatgctat gattataata taatcttatc acaaagtctg
    46021 ttgtaattac aacaaaagca taaaattatt tcgtaaaaat gaggaagata aaatggataa
    46081 taaatacatc aagataataa aaggcactcc cctttttcat ggcatgagcg acgaggagat
    46141 agctcagttt ctcagacctg acggcagtgg cataaaaagc tttcgcaagg aaagctatat
    46201 ttttcacgca ggagacaaga ccgcttattt cggcatactt ctggagggca aggcgcttat
    46261 cgagcgtgac gagctttggg gcggcaggag cataatcgct cctttagcgg caggacagct
    46321 ttttggtgag gtctatgcct gcacaggtga accgcttggg gtgagcgtat gtgcaaagga
    46381 gcaatgcaca gtgctgtttt tatcccctga gtctattata aaggaaagct cgacgctgac
    46441 ggctaggctt ctaaagataa cggctatgaa aaatctcagg ctcaacgaaa agatacaagt
    46501 gataacccct agaactatca gagagcgtgt tatggcttat ctttcttcgc ttgcaaagaa
    46561 aagcggatcg cacattgtca cgacaaattt cgaccgtcaa cagcttgcag attttctctg
    46621 cgttgaccgc agtgcgctgt caacagagct tggaaaaatg caccgtgagg ggctgataga
    46681 tctcaatggt cgggagttta tactgcctga aaactgctct cgtggaacgt gatttgttaa
    46741 tttatatgta aataataagt catttaacca tattcatttt tttgctcttt ttaaagaaaa
    46801 attgcatatt ttatattgac acaacctgct ttgtgttgta taatatatat agtgccgccg
    46861 tgacagttca taattactta aacgtttatt gcggaaatgt atttatttac aaatggccac
    46921 ggtattggga aattgattga aaggattgat tgctttggca aagaagaatg aaagcataag
    46981 cgacaagatc gttcgtttgt ataaggaggg ctactccatt cctgagatag caaaagaaat
    47041 ggagatgcct aaggataatg ttccgagaat acttgaaaag cacttccctg actatgcaag
    47101 ctttgaacag cctgtaagag agggtgctga tgaaaacggc aacggaaaaa agagcgtttt
    47161 caatatgtct gtaagcgagc ttttcggaaa gaagaacaaa aagcaggaga atgttgagcc
    47221 tgcaaaggta actgtaaatc tggctatgga cgatgacgga tttgttgatc acagtgttgc
    47281 ggctatcgcc tcaatgcttc agaaaggcag aagcgtaaac gatatcgcag gattctttaa
    47341 taaagatccc gaggatatca aggctgttca gggctgcatg gacgagcatt tcaaaagaat
    47401 gaatacatac aagggtccaa aggtcgagcc tgcgtctgag cctaaggaag aacctgctca
    47461 gaacataact gatccgctgg cggcaagcct tgcaagcggc attgcaaaga gaaagagcaa
    47521 tatgtatgca acaggtatcg aaacacctgc aaaggctgaa cctgtttccg ctcctgttcc
    47581 tcctgaggac aatagcgttc accttgttac tcctgaggct ctcaaagagg ctgaggaaaa
    47641 agctaaggca gaggaaaatg caaaggctga agctgctgcg gctgaggaag ttcctacaat
    47701 ggacgaggtc gagatgccgt ccatcgagcc tataagcctt gatgacacaa tggtggtttc
    47761 acctgcccct gagtctacta tgccgtctat agaagacgag atagctgaaa agaaagctgc
    47821 ggaagctgaa aatacaaata ataatgagga gggcgagaat atgggtatga gtccaatgga
    47881 aaaaatgcgt atgtttgcgc aggagcagat cgagctgaac aacaagaaga tcgacgagct
    47941 taaggcaagc attgctgaaa ctgaggcaaa gtctttgaat cttggtaagc aggcaagcga
    48001 tatctacgag gagattgtta aacttcagga caaggctaag gcactcaatg ccgagaagga
    48061 gcaggttgat attcagatca gccaggtaaa cgcagagatc gaaggcatta gaaaagaaaa
    48121 caacgaattc aagagctata tgtaatagcg tcttattcaa atcatataag gcaggaggct
    48181 tcggtttcct gcctttttta tttgcgagga atgaagagga aaatagcaca gatgataaac
    48241 gaaagaagca ggacgtcgtc ccctacaggt gatggcaggc tgatgttttt tgattaaagt
    48301 caaacttttt tgcctgaaag tattgaaaaa taacggcaaa tatggtacaa tgcttttata
    48361 aaccttgaaa ggatatttta tatgagcaat atacttattt tggcattcct gttctttgca
    48421 gggtgtctga taggctgggg gatagaagtt attttcagac gatttgagcc taacaacaaa
    48481 gcaagaaaat ggataaaccc cggcttcctt attggtcctt atctgccttt atacggcttc
    48541 gggctttgca ccctttatct gctggcggga cttgaaaaat ttatacacac tgacaacacg
    48601 gctttgcaga agtttatact ttttttgctt atggctattg caatgacagt gatagagctt
    48661 attgcaggcg agatattcat tgtgaaaatg cacgtcaagc tgtgggacta cactggaaac
    48721 aaattcaact ataaaggcat aatatgtccg ttgtattcgc tcatatgggc agcactgggc
    48781 gcggtctatt atttctttat acacccatac attcttgacg cactcaactg gcttgcgaat
    48841 aatttggcgt tctcgttctt tattggattt ttctatggtg tttttgctat cgacctattc
    48901 tattccctga agctggcaga gaagataaaa acgtttgcgg aagagaacga tatggtcatc
    48961 agatacgagg agttcaaacg gcacatcagg gaaagttcag aggagagaaa agagaaatac
    49021 agatttatgc tttccatggg ctcaggtgca aggcttggcg agcatttgaa aaagtatcgt
    49081 gacaaacagc gtgaggagca acgtggacac aggctcaatg actttatatc aaaaataaaa
    49141 aagtcggata catgaaaatt aacaaaataa ccattgacaa aacatcagca tggtgatata
    49201 ctgatttcat atgtaaaaaa cgttgacgaa gacagtaggc tttatgcgaa aatctcagcg
    49261 agccgccgac agtgagaggg cggtatgatt agcttcaggc tgaatatcac ttccgagttg
    49321 caggctgaaa atcagtatga taagtaagct tcgccgcttt tcctgcgtta aagggaacgc
    49381 atatgtcagt atgctgaaaa ggtggtttgc gaatggcttt acggctttcg tcctgtttca
    49441 gggcggaagc cttttttatt gcattttatc tttatgcgta actgatcgtt tattacaaaa
    49501 tcagaaagga ttgatcttcg tgtacaaaaa ggttgacacc aatctgaact tcgttcagag
    49561 agaaaaagaa gtggaaaagt tctgggacga taacaatatc tttgaaaaaa gtattgacag
    49621 ccgtaaaaag ggcgagtcct atgttttcta tgacggacct ccaacggcta acggcaagcc
    49681 gcatatcggt cacgttctca ctcgtgctat caaggatatg ataccaagat accgtgctat
    49741 gaaaggctat caggtacctc gtaaggctgg ctgggacaca catggtctgc ctgttgagct
    49801 tgaggtagag aaaatgcttg gtcttgacgg caaggagcag atcgaggaat atggtcttga
    49861 gccgttcatc aagaagtgta aagagtctgt atggaaatac aagggcatgt gggaggactt
    49921 ctcaggcact gttggcttct gggctgacat ggaacaccct tatgttacct atgacaacaa
    49981 ttttatagag tctgaatggt gggctttgaa gcagatatac gaaaagggct tgctttacaa
    50041 gggcttcaag atagttcctt actgcccacg ctgcggcaca ccgctttctt ctcacgaagt
    50101 tgctcagggc tacaggacag ttaaagagcg ttctgctatc gttcgtttta agcttgttgg
    50161 caaggacgaa tacttccttg catggacaac aactccttgg acgctccctt caaacgttgc
    50221 cctttgcgta aaccctgatg aaacttactg caaggttgaa gccgctgacg gctacactta
    50281 tattcttgcc gaagcgcttc ttgacactgt tctcggcaag cttgcaactg aggacaagcc
    50341 tgcatacaag gtgcttgaaa cttacaaggg caaggatctt gaatatattg aatacgagcc
    50401 gctttatgag tgcgcaggcg aaagcgcaaa gaagcagggc aaaaagggtc actttgttac
    50461 ctgtgacagc tatgtaacta tgtcagacgg tacaggtatc gttcatattg cgcctgcttt
    50521 cggtgaggac gactctaaag tcggaaagaa gtatgatctg cctttcgttc agttcgttga
    50581 cggcaagggc gacatgactg ctgaaactcc atatgccgga atattcgtaa aggacgctga
    50641 ccctgaggta ctcaaagacc ttgacaagtc aggcaagctt tttgccgctc ctaagttcga
    50701 gcatgattat ccgcactgct ggagatgcgg ctcacctctt atctactatg caagagatac
    50761 ttggttcatc aaaatgacag atgtcaagga cagacttatc gcaaacaata acactatcaa
    50821 ctggattcct gagagcattg gtaagggacg tttcggtgac tggctgaaaa acgttcagga
    50881 ttggggcatt tcaagaaaca gatattgggg tactccgctg aacatttggg agtgcgagtg
    50941 cggtcacaga cattctatcg gttctattga ggagcttaag tcaatgtcag acaactgccc
    51001 tgatgatatc gagcttcaca gaccatacat cgacgctgta actatcaaat gccctaagtg
    51061 tggaaaacag atgcaccgtg tatcagaggt aatagactgc tggttcgact caggctcaat
    51121 gccttttgca cagcaccact atccatttga gaacaaggag cttttcgagt cacagttccc
    51181 tgccgacttc atttcagaag ctgttgacca gacaagaggt tggttctatt cactccttgc
    51241 tatctcaacg cttatatttg acaaggctcc atacaagaac gttatcgtgc ttggtcttgt
    51301 tcaggacgag aacggtcaga agatgtcaaa gtcaaagggc aacgctgttg acccattcga
    51361 ggcactggct aaatacggtg cagacgctat caggtggtat ttctacacca actctgctcc
    51421 atggctacct aataagttcc atgacaaggg cgtaacagag ggtcagagaa agttcatggg
    51481 cacgctttgg aacacatatg cgttctatgt gctttatgct gagatagacc agttcgaccc
    51541 tacaaagcat gagatagaat atgacaagct ttctgtaatg gacaagtggc tgctttcaaa
    51601 gatgaactct atgataaagg ctgctgacga caaccttaac aactacagga tacctgaggc
    51661 tgcaaaggct atgcaggagt ttgttgatga tctttccaac tggtatgtaa gaagaggcag
    51721 agagagatac tgggcacagg gtatgtcaca ggacaagata aacgcttaca tgactcttta
    51781 cactgctatc gttacgctct gcaagtgtgc agcacctatg gtgcctttca tcactgagga
    51841 aatatatcag aatcttgtta gaagcgttga caaggacgct cctgagagta tccacctttg
    51901 cgattatcct gtttgcgacg agaacatgat agacgaaaag cttgaagctg acatggagga
    51961 agttctggag atagttgttc tgggacgttc tgcaagaaac ggcgcaagcc ttaaaaaccg
    52021 tcagcctttg agcgtgatgt acgttgcagc agaccaccca cttgacagct actatatcga
    52081 catcatcagg gacgagctta acctcaagga cgttcagttc tcaaatgatg tatctgcata
    52141 tattgattat aagttcaagc cacagctcaa gacacttggt ccaaagtacg gcaagcagct
    52201 tggtgagata agaactcagc tttcacagct tgacggacac aaggctatgg gcgaactgaa
    52261 cgaaactggc agcctgaaac tcacgctttc aagcggtgag atagcacttg acaaggatga
    52321 tgttctcatc gagtctgagc agaaagaggg cttctacacc ctttctgaaa acggcgtaac
    52381 tgtagctatc agcactgttc tcactcctga gcttattgaa gagggctttg taagagaagt
    52441 tatcagcaag gttcagacaa tgagaaagga ttcgggcttt gaggtaatgg atcacatcac
    52501 tgtttctgta agcggcagcg aaaaggttat ggctgttgtt gacaagaaca gagcggctat
    52561 ttctcatgac gttcttgcag acgatatcgt tcttggcgaa aatgtcggca acgaaaagga
    52621 ttgggacatc aacggcgaaa aagtttctat cggcgtaaag aagaactaat tctgagatat
    52681 aaaaaatcaa gcaggacagc tttcgggctg tcctgccttt tttgttttgg attataagcc
    52741 attcacagct cctcctacat aaaaaatcaa tggcgaggtg aaagacctcg ccattgattt
    52801 tttatatcac tttcttgctt ccaaagcgga gatagattcc attatctttg acatcttctc
    52861 ctctgccttt gcaagctttg ccttttcggc ttcgataagc tgttcagggg cttttgcaag
    52921 gaagccctga ttgctgagct ttttgctaag gaagtctata tccttctgtg cgttctttct
    52981 gtccttatca agacgtgcaa gctccttgtc aacgtctatg atatcagcaa gcggaatgaa
    53041 gaatcttgca ctgtctgtaa cagcggcggc gcaatccttt tcctcgcact tatctgtaat
    53101 gatgacctca gatgcagaag caagtctttc aaagaacatt acgccctgtg agaatacttc
    53161 cggctgtgca gtttcaatat aaagctttgc ctttacagac ggcggaacgt tcatttcgcc
    53221 acggcagttt cttatgctct ttatgcctgc gattatcttc tcgaatgttg cctcttcgtc
    53281 ggcaaattca agcttttcgt catatacagg gtaatcctga accatgatag ctgtgccgtc
    53341 atttacgaga gcctgtcaga tctcctcggt gatataaggc atgaatgggt ggagcatttt
    53401 aagcataccc ttcattaccc acacgagaac agcctgtgcg gtagcctctg tttcaccgcc
    53461 tgcggctatt cttggctttg taagttcgat ataccaatcg caatagatat cccagataaa
    53521 gtcataaagc tttgaaactg ctacaccaag ctcgaactta tcaagattat cgtttacttc
    53581 ctttgcaaga gtattgaact ttgatattac ccacttatcc tcaacattga ggttttcagg
    53641 gagcttatca gccttgaaat cgtcaggaag attcatcatg ataaatcttg cggcgttcca
    53701 tatcttattg gcaaagtttc ttgaggattt taccttatcc tcgataaatc tcatatcgtt
    53761 tccaggtgcg ttgcctgttg cgagcataaa tctcaaagcg tctgcgccgt actggtcgat
    53821 gataacaagc gggtcgatac cgttgccgag agatttggac atctttctgc cctgtgaatc
    53881 tctaacaagg ccgtggataa gaacagtatc aaaaggcttc tgcttcatgt tctccatgcc
    53941 tgcaactatc attctggata cccagaaagg aatgatatca tagcctgtta cgagagtgtt
    54001 tgttggatag aaatactcaa gctcctctgt cttgtcaggc cagccgagag ttgagaacgg
    54061 ccacagtgct gatgagaacc atgtatcgag ggtatcaggg tcctgacgca tttccttgcc
    54121 gcacttaggg caacaagcat ggtcttcctt tgttaccaca gtttcaccgc aatcgtcgca
    54181 ataaaatgcc ggtatctgat gaccccacca aagctgacgt gagatacacc aatctctgat
    54241 atcgttcatc cagttaaggt agttcttttc aaatctttca ggaacgaact tgatagagcc
    54301 gtcctcaaca gcctttattg caggctttgc aagctcgtcc attttaacga accactggag
    54361 ggatactctt ggctcaactg ttgttccgca gcggtagcaa gtacctacgt tatgctcgtg
    54421 gtcctcaaca taagcaagga agccgccctt ttcaaggtct tcaactattt tctttcttgc
    54481 ttcgtatctg tccatgcctg catactcagg ataatcgtca actatctttg cgtcctcagt
    54541 aagaacattg ataacaggca ggttatgtct tttaccgact tcaaagtcgt tagggtcatg
    54601 ggcaggagtt atttttacaa cgcctgtacc gaactccata tcaacatagc tgtcggcaac
    54661 aacaggtatc tctctgccaa caagcggcag tgtgagtgtc ttgcctacga tatccttata
    54721 tctttcgtcc tttggattta ctgcaacggc agtatcgccc aggagcgttt caggacgtgt
    54781 tgtagcaagc tctacatatc cgcttccgta tgtgagagga tatttcagat gccagaagtg
    54841 accagcctga tcttcatact caacctctgc gtttgagata gatgtacggc aatgcgggca
    54901 ccagtttatt attctttcgc ctctatagat aagtccctta ttatagtagt ttatgaaaac
    54961 ttcctttaca gcctttgaac agccctcgtc cattgtgaaa cgctctctgt cccaatcgca
    55021 ggaagagccg agctttttaa gctgctcaac tattctgccg ccgtatttct ttttccaatc
    55081 ccatgctctt tcaaggaaac cgtccctgcc gatatcgtcc tttgtaagac cctctttacg
    55141 catagcttca actatctttg cttctgttgc gatagaagcg tggtctgtac caggtatcca
    55201 ctctgcggca tatccgctca ttctcttcca gcggataagt atatcctgca aagtttcatc
    55261 aagggcatga cccatatgga gctgtcctgt gatgttcggt ggtgggataa ctatacagta
    55321 tggctctttc ttggaatcag gctcagcgtg gaaaaatccg ccgtcaagcc aatatttgta
    55381 gatcttatct tctaccttat taggctcata aagctttgaa agctcttttt tcattataaa
    55441 acaattcctt tcgcaaatat ttttctatca cagtatcgtg gcaagaccaa gaggtgttgc
    55501 ctttttgccg tcagactcgt gtcttaggct gtctgcgaaa cggaactgtg aatttcattt
    55561 gaaagctgaa tttgagatca cacattatat tctttccttt ccgcatgata tggcattact
    55621 gcacctatta taatattatc tcacttttgc accgaattgt caagggctga tgacggtatg
    55681 gaacgaaaaa agcagaacag cttgaaactg ctctgctgag agtgttgaaa aagactcaaa
    55741 caagcgggac gtcgagggcg ccgtccccta caacttgcat aaaaacgaat ttacgcttac
    55801 agcttgaaat gaacgggcat accgtttttc attgctattt ttacctcacc ttggataagt
    55861 ggcaggaagt atcttacggc ttccggcttt acgtcattgc cgtcctcgct taaccactgg
    55921 gcagggaaga atttttcctt atttgcagca tcatgggcgt caacgctgtc aacggtcaca
    55981 acataaggca cgtcgctgat acgcttgaat atcatcattt tacctgtttt ccctgcaaga
    56041 gccgctttta cgcctgctga gcctatttcc tcggcttcgt ctatatccgt ctttgagcag
    56101 atatgagaag aacatctctg cataacgtta agctcaatag aacggacttt acagccgata
    56161 ttctctttaa cgagctgttc aagggtcttt cctatgccag agagatactc gtgaccgaaa
    56221 ttatccacaa ctctcggctt gttttcctcg cccttaagct ttacgccctc ggaaactgcg
    56281 atgataacgg ctttgtgctt tgccatttga tttcttatgt cctccaggaa cttttcggcg
    56341 gtaaagttat tttcaggaag atagataagg tgaggggcta tctcgtcatt tgctctgagt
    56401 acgcaggtgg aagctgtgag ccagccagcg tgtctgccca ttatctcaac gatagtgacg
    56461 gactcgatag aataaaccga gctgtcacgg attatctcct gaaccgttgc ggcaacatac
    56521 tttgcggcag agccgaagcc tggtgtatgg tctgttacag gcaggtcgtt atctatagtc
    56581 tttggtatgc ctataactct tatctttgaa tttcttgccg caagataatc ggaaagcttt
    56641 gccacggtat ccatagaatc gtttcctccg atataaaaga aagcttctat cctatgcttt
    56701 tcaaggcagt tcaagatacg ctcatatgtt tcaccgccct cctcaacatc aggaagttta
    56761 taacggcatg agccgagaac tgttgacggg gtctgtctta gaagctccat atcctcgttt
    56821 gtttttataa caagcttaag atctatgagc tggtcgttta tgataccctc gataccattg
    56881 agcgagccga aaacggcgtc tatctgtggt gttttcacag cctgtttgaa aactcccacc
    56941 aatgaagcgt tgatagcgca ggtaggtcct cctgattgtg caacggcaa
//
LOCUS       DAWEMR010000002        53395 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_288_length_53395_cov_14.277053, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000002 DAWEMR010000000
VERSION     DAWEMR010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 53395)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 53395)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..53395
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_288_length_53395_cov_14.277053"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7029..7038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20461..20470
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    43756..43765
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        14189 a        12941 c        10715 g        15520 t
ORIGIN      
        1 gagcttccat atcgaggaaa tccacgggct ttgttgcgct tttttttaca gcgcccattt
       61 tttataccct atttatcttt cagcgcctca gaaagcgtcc gccagccaag cattgcacac
      121 tttactctcg caggcatatg ggaaatatcc ctgagtgcag aagcttcttc aaggctgtct
      181 atctcttcgt cagacgcctc gccccttatc attctcatga aaatatccgc atattccaaa
      241 gccttttcct tgctctgacc tattatcatc ccaagcatta tatccgttga agcctgagat
      301 attgcacagc cgtcaccaac aaaagccccg tccgtaatga tatccccgtc agttttcagc
      361 tttaactgta tctcgtcgcc gcagcttgga tttatcccgt ccagcacaat gtctgcgtcc
      421 tctatatcat gcttgaaatc agggtgcata ttgtgctccg ttagtatctc attgtaaaaa
      481 tttctatttt ccataaccca ttcgctccct aacagttgaa agactgtcta aaaatctgtc
      541 tgcgtcctct ttggtattgt aaaaagcaaa gcttgccctc actgttgaac gatagccaag
      601 gtattcaagc agcggctgtg cgcagtgatg acctgccctg accgcaatgc cgtctgccgc
      661 aagtatctcg ctcacgtcat gtgggtgaac attgtccaca gtaaaagtca aaataccatt
      721 atgctcctca gctttctccg aaccaagtat atttatgtga gcaatctctt tcattttctc
      781 aaaggcatac gccgaaactt caagctctct cttgtgtatt tcctcaaagc ccacgctttt
      841 cacatagtct atagcagatt tcagtcccac tgcacctgcc gcattgacag tgccagcctc
      901 aaacttatgg ggcagttcag catacaccgc cgaagtcctt gacaccgact ctatcatctc
      961 gccgcctgtg agaaacggcg gcattttttc gagcaactca cgcctgccgt aaagcacgcc
     1021 tatgctcata ggtccataaa gcttgtgacc tgaaaacgca aagaaatcca ccccaagctt
     1081 tttcacgtcc accgcaatat gcggcgtgct ctgtgcgccg tctgccacca taacagcgtt
     1141 cttgcggtgt gcaatttctg caagcttttc tataggatat tctctgccaa gcacatttga
     1201 aagctgcgta acagccacta tcttggtgcg gtctgttata agttcctcag ccttgttaag
     1261 gtcaagactg ccgtcctttt cacattcaag gaatttaagc tccgcacccg tctgcctgca
     1321 aaccatttgc cacggcaaaa ggtcgctgtg gtgttccatt atggaaacca ccacctcgtc
     1381 gccctttttc aaattggaaa gcccatagct gtatgccaca agatttatgc tctctgtggt
     1441 gttgcgtgta aaaattatct cctgcacaga cttcgcaccg ataaagtcac gcactgcctt
     1501 tcgtgcattc tcataatcct ccgttgcctg aacgctcagc tcataaattc cacggagagg
     1561 gtttgcattg tggcaggtgt aaaaatcttt ttcagcgtca ataacgcatt tcggtcgctg
     1621 tgcagttgca gcgttgtcga tataaaccgt tttatcctgc ataagaagtg gaaaatccgt
     1681 cttgtatccc tgcattgtcc tcattcctct gtatcagttt ttattcccat ggctttttca
     1741 gccaccgccg caaattcgct gtcctcagcc atatggataa gcctttcaac agcggcgtaa
     1801 gccattatct tctcggcagt ttctctgtca atgcccctgc tctcaaaata gaacatagtg
     1861 tcagcgtcaa gctcgcctat agtcgctccg tgagtaccct caacgttttc ttctgcacaa
     1921 agtatcagcg gaacagtttt gttcacaaca tcgtccccaa gcatgagcac agtttcgttc
     1981 tcgctgccaa cagagtccgc agagcctttc ttaaaatcta tcgttcctct gaaaaccttt
     2041 tttgcactgt ccgtcaaagc gccgttcaca gtgataaggc tctccgtctt tttgccgaaa
     2101 tggtcagcca ctatgttata gtctatagtc tgtgaatttt tccccagata agcggcgtca
     2161 gcgtcaaacc tcgctccgtc accgctgaga gtaacgtggt cgtcactgta aatatctcct
     2221 ctgcctatca taatagtcat aatgtggata tgggaattta ccccgcattc cgccctgacc
     2281 tcatgtcgca caagtccgtt ttccgacggc tcaagaagca taacgagctt cacctcagct
     2341 ccctcctcaa ggtcgatatt cacccttgac agcataggag cgtttcctct gcaaacttca
     2401 aaaataacgc cccttgcggc cttttcagcc gttacgttca cgacattttc agtatatccc
     2461 tctgcgccgc tgttgtcaag ccttacaggc tcgttctttt catctttttt taagatgatg
     2521 ttttcctcag ttttcgctgc actttcgctg tcccactgta tctcagcact gtttaccttg
     2581 aggaaattcc acgtttttgt cggcagcttg tctatcagga tatttttagt cattttaagc
     2641 actcctttcc cgtcagccta tgctgccttt catttcaagt cttataagat tgttcatttc
     2701 caccgcatat tccacaggca gttcctttga aacgttgtcc gcaaagccgc ttacgataag
     2761 agcccttgcg tcctcttcgg tcatgccacg gctcataaga tagaaaactg cgtcgccgct
     2821 tatgctgcct atcttcgcct cgtgaccgat agcggcgtcc tttgtgcgga tatccattgc
     2881 agggatagtg tcagaccttg actcagagtc aagcataagc gactggcatg ataccgatga
     2941 cttagccttt ttcgcactct tttccaccac aacgctgctc ctgaatgtgc ttattccgcc
     3001 actcttgctt attgaacgtg tgttcatgaa ggagcttgtg ttcttgccca catgaactac
     3061 ctttgcgcct gtatcgagat tttgtccgct tcctgcgaaa gtaactcccg tgaactccat
     3121 tttggaattg tcgcccttta aaatgctcat agggtaaagg cagccgatat gcgaaccgaa
     3181 tgaacctgag acccattcga tagtgccgcc ctcttccaca agggcacgct ttgtattaag
     3241 gttatacata ttcttcgacc agttctcgat agtagaatat cttagctttg cgttcttctt
     3301 aacataaagc tcaacacagc ctgcatgaag atttgcaaca ttgtattttg gagcagaaca
     3361 gccctcgata aaatgcagac tcgcaccctc gtcaactatg ataagcgtgt gctcaaactg
     3421 acctgcaccc tttgcattaa gtctgaaata agattgaagc ggtatagaca gctgcacgcc
     3481 ctttggcaca tacacaaacg agcctcccga ccacactgcg ccatgcaaag ccgcaaattt
     3541 gtgatccctc ggtgtaacaa gcttcatgaa atattttctc accatatcgg catattcgcc
     3601 tttcatggcg ctttcgatat ccatgtaaac aacgccctcc tgggccacct cagagcgcac
     3661 gttatgataa acaagctcag agtcatactg cgtacccacg cctgcaagag attttctctc
     3721 cgcctgcggt atgcccagcc tttcaaaggt gtcctttata tcctgcggaa cgttcttcca
     3781 gttgttctgc attttcgtgt ttggtcttac atatgtggca atattgtcca tatcaagccc
     3841 ctcaagtgac ggaccccagt tcggcgttgg tatctcgtta tagatctgca aagactgcaa
     3901 tctgaactgc tgcatccata cagggtcgtt tttctctttt gatatcttct ctattatttc
     3961 aggcgtcaga ccgtttttta tgcggtaagc gtcgttttca acatctttta tatcataaat
     4021 actgcggtca atatccatga cctgactttt ttctttcatc attttcacct cgtcctctta
     4081 ctgcgtgtaa tggcaaaacg ccccttacac acttttatca gatagccgaa aagccctttt
     4141 cgtttatctt ttccacaagc tctgcaccgc cgtttttaac gataacacca ttttccataa
     4201 catgagcgac atcaactgta agagcctcca gtatccttgt gctgtgtgtt ataataagca
     4261 gtgagccgtg aacacgctct ctgtagagct ttacgccctg tgaaactgtc cttactgcgt
     4321 caacgtcaag gcctgagtct gtttcgtcaa gtatggcaag ctttggctca agcatgagca
     4381 tttgcagtat ctcagccttt ttcttctcgc cgcctgaaaa gcccacgttc agatctctgt
     4441 cagagtaaga ctcgtccata ttgagaagct tcattgtttc cgcaagcttt ttcttgaaat
     4501 cccaaagtct aagacgtgtc tttgttttct gctccaaggc acttcttata aacgagctca
     4561 gtgtaacacc aggcacttcc agcggatttt ggaatgacag aaaaatgccc tttttcgcac
     4621 gctcgttcac aggcatatcc acaatgcttt caccgccaaa aacaatgtca ccctctgtta
     4681 cagtgtaagc agggttgcct gttatagcat aacccagcgt agatttacct gtgccgttag
     4741 ggcccataag aacgtgagtt tcatcactgt tcaccgtcag gtcaacaccg tgcaaaattt
     4801 ccttatcccc aacgctcaca tgaagtccag ttatcttcaa taaattatta cttttcatat
     4861 tctgtgtcct ttcctctatc gccttgtcag gctttgtaaa acgtcatgac atggttatca
     4921 cgagttagct gttcctaata gatattatat cacaaaaaca tagaaaatca ataggaattt
     4981 tcaacaaaca attattctaa actaactcac ataaagtgat gataacaaaa aaatcgattt
     5041 taccccttga caaataatat tatatgacgt ataatatatt caacgatata gtattataga
     5101 tcaatataac aactgagctg actaaagaga ggagagagta aatgggcttt ccaataagtg
     5161 caggactgct tgattccatg gtactgtcag tggtagacag agaggatacc tatggatatg
     5221 agataacgca gttcatacgc agtgcggtgg acgtttcaga atccacattg tatcctgtgc
     5281 tgaaacggct tcaaaaaaac gagctgcttg aaacctacga taaggaatat atgggcagga
     5341 acagacgtta ttatcacgtt accccaaagg gagaaaaagc tcttgaagaa taccgcagag
     5401 agtggaagaa gcataagaat atggtagacc gtatattatt agaaaaaggt ggagaaaaca
     5461 catgaacaga cttgagtatc ttacccaact gcgcagatcg cttgaagacg gcggacttgc
     5521 cgaagatgaa ataaatgacg caatggggtt ttatgaggag atattccttg atgcaggtgc
     5581 agcccatgaa gctgaaactg ccgcaaacct cggctcgccc gaagagcttg ccaacaaaat
     5641 tttgcaggac agcggcatac acccacaggg cgactcggta tttcaaatgg aagccgccgc
     5701 tgacccctca caggctcgtg atactgacaa taatgtgcca cagaacggca attctctctc
     5761 aaagcttctt gttatcatca tcacaagccc aatatggttc agccttatgt gtgcctttgc
     5821 cgcaatagcc ttggcgatac tttgtgcact tttaagcata ataattgcag tttttgcaac
     5881 aggcttctcc cttacaatag gcggtatagt aacgcttttc acggtctatc ccgcaggcat
     5941 tgccctgatc ggtgcaggac ttatttgtat cggacttgga ggcatgattg taatgcctgt
     6001 tataaggtgc atatggcgtg gctgtgtaag ccttttcaac aaattcacaa actttgtcca
     6061 taggtttgta agctcaaagg cggtggcata atggcaaaat atgataacac aggcgacgta
     6121 aaacgcagac gtttcccatt ttttttgacg atatttatta tcggcatagt gcttttcatt
     6181 gtgggcgtgg tgcttatcaa ggtcacaaac accaaaagcc ttggcaagcg ttattcctac
     6241 aacgaaactt ttgacgttgc cactatagat aatatggaga tcgacttctc cgcaggggat
     6301 ctcaacatca aaaagagcag tgacgaaaat atccacgtca caggagaaaa tctcccggag
     6361 aagataaatt tttcgtcatc aaacagcaag cttcaggtca agaccgccga cagccttttg
     6421 caccttaaag gcggatatat ctcctttgac ggaagtcagc ctacgctgac tatagagctt
     6481 cctgaaaaag cgtttgacac tgtaaagata gacgcaggtg caggacaaat gaagctttca
     6541 gatctgacct gtgaaaacct cgacatagat ttcggtgcag gaagctcaga ggtcattaac
     6601 gtcacctgca aaaatgaagc cgactttgac cttggagtag gaaaaacagt tttcaaagac
     6661 tgcactttta acgattccga ctttgactgc ggagtaggcg aaacagactt ttcaggaaag
     6721 cttctcggcg acgctgagat agacggcggc ataggaagaa taaattttga tattgacggc
     6781 tgcaaaaacg actataagat aaaatcagac agcaaagtga aaataaatag cacaacagaa
     6841 gagaacgagc ttctctacaa aataaaactt gacattgaca acggtatcgg cgaaatttcg
     6901 cttaatttca gataaggagt gatagaaatg gcaaagccac tttacaaatt aagagaaggc
     6961 aggataattt caggcgtttg cgcaggacta gcggagtatt tcaacatcga cgtgaacata
     7021 gtccgtatnn nnnnnnnngt gcttataggc tgtgcaggcg gtgcaggagt tatcgcatat
     7081 attgcggctg caatgcttct gcctgagaaa taagaccata ctaaaaacaa aaaccacggc
     7141 tgaatgtaaa ctcagccgtg gtttttttac gtcttaacaa atatcttttc tgttcatgaa
     7201 agtatgtgac caatgcacaa aattgccgca ccaaaactgt gcaaacaaat gatttatatt
     7261 ttaatttggc aacaattctg caattgttgc attttttatg caacaaaaca cctctttttc
     7321 gggataagta ggaggtgata aacatggaag cagtaatagc ggcgttcata gcggcggcag
     7381 ccagcatagc ctgccagctt ataatagctc gttcgggagc taaagaaaac aaaaaatcac
     7441 aggaagccac cgaacggctt attatatata gaatagatag gctggaacaa acagtttccg
     7501 agcataacgc actcatctca agaatggcgg cagcggaaca aaagataaca gacatggaaa
     7561 gggagagata ttcatgaaag aaaaactcgc aaagctcatt gacgtaaaat cgcttgtcac
     7621 actgcttatg acaagcgtgt tctgtatcct cacgctgaaa gggatagtca gcggagagca
     7681 gttcatcaca gttttcactg tggtgatatc attttacttt ggcgtacaga cggcaaaaaa
     7741 atctggaggt gacggctatg gaaaaagcta agggcataga cgtttcccat tggcagggaa
     7801 gaatagattt tgacaaggtg aagtcccagg gttacagctt cgtgatgata aacgcaggct
     7861 acggcaagta catcagtcaa aaagacgaaa atttcgagaa gaactatgcc gccgccagaa
     7921 aggctggtct taatatcggc acatactggt attcctacgc ccttacggaa gccgatgcac
     7981 ttgcggaagc gaaaactttt cttgaagcgg tcaaaggcaa aatgtttgaa tatcccctcg
     8041 cctttgatat cgaggacgct tcccaaagtg aacttccaaa cgctgccata aacaaaatta
     8101 tagaagcctt ctgcgactat ctcgaaagca acggctacta tgcggctgtc tacagctacg
     8161 caaacttttt caagcgcaag gtatcagact ccgtaaaaag ccgctacgac atttgggtgg
     8221 cacactttga cgttgcaaag cctgccatat caaactatgg aatgtggcag tatacaagca
     8281 aaggaactgt aaacggtgtt tccgaccgct gtgattgcaa ctacgcctac aaagactacc
     8341 ctgccataat gaagaaaaag tgccttaacc tttacccttc caacgcaaaa aatctcgata
     8401 ccacaggcta caaaaaaggt gataaaggca atggtgtcct tgcactgaaa tatcttctta
     8461 tgcttgcaaa gaaaaagggt atgcacaata tcaaccttga taaaaacgat attttcggtg
     8521 caggcactca gaaagccgtg aacaatatac tcaaatccca cggcttttcg caaaacggca
     8581 tagcaggcaa aaagttcatt gacctcattg gcaatgaact tctaaaataa ccacaaacca
     8641 tagccacact tgtaggggcg aacaacgttc gcccgcatat cttgatacca atgcccaaat
     8701 ataagcacat aaaaaaggct gtacccttac ggatacagcc ttgattgcta tgcaaaattt
     8761 attaaaccaa ctgaatgata gccatttcag cagcgtcgcc acgacgtggt ccgatcttga
     8821 tgatctttgt atagccgccg tttctgccct cgtatgaagg tgcaatgttg tcaaacagct
     8881 tcttagctac gtcttcctta gttacataag aaagtacctg acgctttgag tgaaggtcgc
     8941 ctgccttgcc gagagtaatc attttctcag tcatagcagc aacttcctta gctcttgtta
     9001 cagtggtttc aatcttaccg ttttcgagaa gataagtaac catagctctg agcattgctt
     9061 ttctctggtc gctagttctt cccagctttc ttgtgcctgg catagatttt cactccttac
     9121 tttaattatc gtcctctgaa ttaaggtcgt aacccaatga atgaagcttt tccttgacct
     9181 cgtcgaatga cttcttacca aggtttctta ccttcatcat ttcttcagca gacttttcaa
     9241 tcaaatcgtt aaccgtgtta attcctgcac gcttcagaca gttaaatgaa cgcacagata
     9301 agtcaagttc ctcaatggtc atctcaagga tcttttcctt gcccttttca tccttttcaa
     9361 ccatcatctc tacaacgtta gtctcttctg agagatcaac aaagagattc agatgctcgt
     9421 tgaggagttt ggctgccatt gatacagctt ccttagcgga gatagtaccg tcggtccaaa
     9481 cctccaatgt cagcttatca aaatcggtga tctgtccaac acgggtgttg tcaaccgtga
     9541 agttcacctt taatacaggc gtataaatac tgtctactgc aataacacca ataggggcgt
     9601 taggcataag cgccttgttc ttttccgcag aaacatatcc acgacctctg tcgatgacaa
     9661 tttccatata aagctttgcg cccggtccca aagttgcaat gtgcatatca ggaacaagaa
     9721 tgtccacctc agaatctgtc ttgatgtcgc cggcagtgac aacgcactcg ccctcggcct
     9781 caatgtagat agtcttagga ccctcgccgt aaagctttgc agttaaaccc ttgaggttaa
     9841 gaatgatctc agtaacatct tccttaactc cgggaattgt tgatagttcg tggtgtacac
     9901 cgtcgatctt taccgatgta acggcgacgc ctggcaaaga ggagagcagt acacgtctca
     9961 gactatttcc gagcgtaata ccatagccac gctccagagg ctcgagaata aacttgccgt
    10021 atgttccgtt gctctttagc tcggctgttt cgatttcagg cttttctatt ttttcaagca
    10081 tttaagaccc tcctgttttc aaactcgatt gttttatatg atcaataaca catacacttc
    10141 caaaaacata tcaagtattt cttcgatacg cactcttgaa gagtgcatac cgtaagaacc
    10201 actatcaata cattatttag aatacaactc gacgatgagg tgctcctcga tgtcatagtc
    10261 aaggtcttcc ttaacaggca tacgaagaac cttagctgtc tggttgtcct tgtttacatc
    10321 aagccatgta ggagctgcct ttgaagcgtt agcctcaatg atctgcttga acttctcgct
    10381 cttcttgctg ttttccttaa gtgagataac atcgccttcc ttgattctgt aagaaggaat
    10441 gtcaactctc ttgccgttaa caagataatg accgtgaaca acgagctgac gagcctctct
    10501 tcttgtcatt gaaagaccca ttctgtatac aacattgtca agtcttgact cgagaagagt
    10561 aatgaggtta gcaccggcct gaccctccat tttctcagca agctcgaaca ggtgacggaa
    10621 ctgcttctca agaacgccgt agatgaactt gagcttctgc ttttccttaa gctgcatacc
    10681 atattctgaa actttctttc tcttgttagc gttaggatca cgctttgttt ccttagcgat
    10741 acccataact gctgggctga ttccgagaga tttacatctc ttgagtattg gttctctatt
    10801 tactgccatg gtaaatacct ccgttttcta aatgttatcg ggtcttagcc gaattgctta
    10861 cccgactttg agtaaactac aaactcaaat tgtcttataa actatacacg tcttctcttt
    10921 ggaggacggc atccattgtg agggatcgga gttacatcct tgataagtct aacttcaaga
    10981 tcctcagact gcaaagctct gattgcagcc tcacggccag caccaggtcc ctttacaaaa
    11041 acttcaactg tcttaagacc atgctcctta gcagctcttg cagcagtttc agcagctgtc
    11101 tgagcagcga atggagtaga cttcttagaa cctctgaagc cgagtccgcc ggcagatgcc
    11161 caagaaatag cattgccctg catatctgta atagttacga tagtgttgtt aaaagtagac
    11221 tggatatgaa cctgtccctg ttcaatgttc tttctttcac gacgacgacg aatagtagtc
    11281 ttcttcttag cctgagccat tgttcacacc ctccttactt cttcttatta gcgattgtct
    11341 ttacaggacc cttacgagtt cttgcgttgg tctttgttct ctgacctcta actgggagac
    11401 ctcttctgtg acgaagacca cgataacaac cgatttctgt caatctcttg atattgagcg
    11461 caacatttct tctgaggtca ccctcaacag tgatgttatg gtcaatatat tcacgcaatt
    11521 tagctacatc gtcctcagac atatccttaa ctcttgtgtc aggatttacg cctgtttcat
    11581 taagaatctt agtagcagtt ttctgaccta taccatagat ataagtaaga gcaatttcaa
    11641 ttctcttatc ctttggcagg tcgataccag caatacgagc cattatttag ctgcacctcc
    11701 attattggtt tgttagccct gacgttgttt gtgcttagga ttctggcaga ttaccataac
    11761 cttgcctttt ctcttgatta ccttgcactt ctcgcagata ggcttaactg aaggtcttac
    11821 tttcattgaa atacctcctt taaatttagc gacaagaatt aaaaattctt aaacttacgg
    11881 gaaagcttac ttagctctcc atgtgattct tccctttgtc aagtcatacg gtgacatttc
    11941 gaccgttacc ttatctccgg gtacgatacg aatataattc atacgcagct tacccgaaat
    12001 atgagcaagg attttatgac cgtttgaaag ttctaccaga aacgttgcat ttggcttagc
    12061 ctcaattacc gttccctcaa gttcgataac gtcttccttt gacattaaaa aacctcccca
    12121 tctaaaaggt tttggtctgc tgtaacacgg tttgggtcaa aaccttgtat cagccgtctt
    12181 aatttcctgt ttgtcagctc atcagtgctg atgttgacgc ccgcaggtga aatgtgtttt
    12241 atgtttttac gctttggctt ttcaagctta cgctccttgc cgtctgcgat atacacaaaa
    12301 ccgccgctgg cagctaccgc aacaaaatat ctgcccttat cacggcctgc gttcgcaagc
    12361 accactgaac cgatcggtat atctacaacg cttttctccg tcatactttg gtcaggataa
    12421 ctgccccgtc aggagtaact gcgattgtat gctcaaaatg agccgcccac tttccgtctt
    12481 ttgtcttgac agtccaaccg tcaggcattg tcttgatatc agccgtaccc atgtttatca
    12541 taggctctat ggctattacc atacctgcca caagtctgat gccgtgacca gccttgccat
    12601 agtttggaac ttctggatct tcgtgcaggt ttttgcccac accgtgacct acgaactgtc
    12661 ttacaacgga gtagccgttg tcctcattgt atttctgaat ggccgcaccc aagtcgccaa
    12721 gtctaacgcc cggctttacc atatctattg caagatacag actttcttcg gtggacttca
    12781 tcagagcctc tgcctcaggg gaaatcttgc ctacagcaaa ggtcttgcag gaatctccgt
    12841 gatagccgtc aatgtaagca ccaacgtcta ctgaaactat gtcgccctcg caaagcttgc
    12901 gtggaccggg gataccatgg attatctcat cgttgacgga aatgcaggct gagcctggaa
    12961 atccgccgta gccaaggaag cttggctttg caccatgaga agtgatatat ctgtgaacga
    13021 ctttatcaag ctcgtaggta gtcatacccg gtctgatagc ctcgcccgca gcgatcaatg
    13081 ctccggcggt tattgcgccc gccttctgca ttttctcgat ctctttattg gatttaacgc
    13141 atatcattaa aaacgcctcg gatctatgcc ttaacagctt cgagaacaag ctttgaagtg
    13201 tcctctacct tatcctgtcc ctcaacggta acgagcttac cctgcttacc gtagtaatct
    13261 ctaagaggag cggttgattt gtgatatgtc tgaagtctgc tgataactgt tgctggctcg
    13321 tcgtccttac ggataacgat cggctttccg caaacatcgc acacgccctc tacctttgaa
    13381 ggcttgaaag ctgtgtggta agtagcgcca cagtcgaggc aaactcttct gcctgaaagt
    13441 ctctgctgga tagtctcatc aggaacatat atctctatga ccttgtctat cttaacgccc
    13501 atagcgtcaa gagcctcagc ctgaggaact gttcttggga aaccgtccag aatgaaaccg
    13561 ttctgagcgt caggctcagc gatcctgtcc ttaaggatac caattacgat atcgtctgat
    13621 acaaggtcgc ctgcgtccat agcagcctta gccttgagac cgtactcagt gccagcctta
    13681 acagcagctc tgagcatatt gcctgtagaa atagtaggaa tgttcagagc cttgcagata
    13741 acctcagcct gagttccttt tcccgcaccg ggagcgccta aaagaataat attcattttt
    13801 aactcctttt tatctcaaac gtaaagccta tggtttattc aaggaagccc ttatgatgac
    13861 gcatcatcat ttgagattcc atagttcttg cagtttcaag agcaactgat acaacgatga
    13921 gtatcgaagt accgcccatt gcaatattca tctttgtgat cgaagtgaag ataataggga
    13981 agatagcgat tattccgagg aacacagctc ctaccaatgt tatctttgac agaaccttct
    14041 taagataatc ggaagttggt ctaccaggtc tgataccagg gataccaccg ttgttctgtc
    14101 tgaggttgtt tgcgatctca agtgggttat actgcattga aacatagaaa tagttgaagc
    14161 cgattatcag cacaaagtac agacaagcgt agaacggatg agtgtactcg aaccagtgaa
    14221 ggaagtgatc gtagaagctt ccgctctttg gttcaatgaa cattttgagt gttgaaggca
    14281 gtgacatgaa agccattgca aagatgatag gcataacgcc gctcattgcg atcttgatcg
    14341 gaataaaagt attctgaccg ccgtactgtt ttctgccgac tactctcttt gcgtactgta
    14401 tcggtattct tctttcagcc tcgttcatga agatgataaa gcctatcatc agaacgaaca
    14461 caacaaggat agcaattacg agaatgatgt tcttagcctc gcctgtcttc atgtaatcga
    14521 acagaccgat aactgctgaa ggacctcttg caatgatacc tgcgaacagg agcattgaga
    14581 taccgtttcc gatacccttc tcgtttatct cgtcaccaag ccagatcgtc agtgacgcac
    14641 ctgctgtaaa gcaagcgata ataactatct ctgcgaatat cttgctaaag ccctctgtgt
    14701 aaacacaagc acccgctttc ttcatggtga ggtaataagc ccacgcctgg aagatagcaa
    14761 tagcaagtga cgcatatttt gttatcttgt taagtttctt tctaccctca ggaccttcct
    14821 tctggagtct ttccagcggt gggagagcgt aggtcagaag ctgaatgata atttgtgcat
    14881 tgatataagg tgaaacggag agcgccaaga gagtagcttt tgaaaagtta cctcctgaca
    14941 gaacgttcag atagctgaaa aagtcacctg acgaagcact ctgttcaaac catgctgaca
    15001 atgcgactga gtcaaggtaa ggcacaggaa ctgtaccgcc gactctgtag atgatgatga
    15061 tgaaaaaagt aaatagaagt ttttttctta attccggaac tctccaggca ttacgaaatg
    15121 tctctatcac aattacatca cctcaacctt cccgcctgcc ttttcaatct tttccttagc
    15181 agctgctgag aacttagcag ccttaacggt aaggttttta gttaattcgc cgtttccgag
    15241 gatcttgacg ccgtcaagtt ccttcttgat gatgcctgaa gctttaagaa gctcagtatc
    15301 aacaactgtg ccgtcaacaa atctatcaag atcagataca tttacgatag catactttgt
    15361 tgcgaaaata ttgttgaatc cacgcttagg gattcttctt gcgagcattg tctgtccgcc
    15421 ttcaaagccc ggtcttattc cgccgccgga acgagccttc tgacccttgt gtcccttgcc
    15481 tgcggtcttt ccgttaccag aaccgtgacc tctgcccttg cgcttgctct cctttacgga
    15541 gcctgcaaca ggtgataatt cgtgcagttt cattcagatt gcacctcctt atgcttcagt
    15601 tactttaata agatgggaga taacctttac ctttcccttt gtctgaggat tgtcaggctg
    15661 acaagtctta tccccgattt ttctgagacc cagtgaatta gcggtagcga cctgcttatc
    15721 gcttcttccg ttaaggctct ttatcagttc gatgtttatt gccataactg ttatcctcct
    15781 tagcctgtaa tttctttaac tgtcttgcct ctgagagctg ctactgtttc agcgcttctg
    15841 atgttagcaa gaccgcttat tgtagctctt actgcgttgt aaggattgtt tgaacggagc
    15901 tgcttagctc tgatatcctt aattccagca gcttctacaa cggctcttac tgtgccgcct
    15961 gcgataactc cagtacctgg tgctgcaggc ttgagaagaa cctcgcctgc accgaactta
    16021 ccgataattt cgtgtgggat cgttgttccc ttaagtgaaa ttctgataag gttcttcttg
    16081 gcgtcctgga ttgcctttct gattgcgtca ggaacttctc ctgatttacc gattccgaaa
    16141 cctactactc cgtttccgtc gcctactact acgaggactg agaacttcat tatacggccg
    16201 cccttaacag tctttgatac tctgttaatt gcaacgacct tttcagtaag ttccatgttt
    16261 gaagcatcta ttaaagccaa tgtttaccct cctctttaga acttaagtcc gccttcacgg
    16321 gctccgtctg ccaactcctg aacacgtccg tggtaaaggt agccgcctct gtcgaatacg
    16381 acattctcga tgccctttgc cttagccgcc tgagcgatca tttcgccgac cttacgagcg
    16441 ccttccttgt tgccgccgtt gccttcaaag tccttggaca ttgaggaagc gctgcaaagt
    16501 gttacgccgt ttacgtcgtc gataacctgt gcataaatat gcttagagga gcggaatacg
    16561 ttgaggcgag gacaatcagc tgttccgttg atcttgttgc ggactctctg atgtcttttt
    16621 gctcttgcct tagccttgtc aagcttttta accatagtaa ttctctcctt ttattacttc
    16681 ttgctgccct taccagcctt accttcctta cggataacat actcgccttc gtaacgaata
    16741 cccttaccct tataaggctc tggtggacgc ttctcacgga tctcacaagc aaactgacct
    16801 acttcctgct tgtcgcaacc tgaaattacg atgtggtttg cgtcaggtac atcgatcttg
    16861 atagtatctg tttcaggtac gattacctga tgagaaaaac caaggttcat aacaaggttc
    16921 ttaccctgct tctgtgctct gtaacctaca ccaacgatct caagcttctt tgcgaaaccc
    16981 tctgtaacac cctctaccat gttgtgtacg agtgttcttg tcagaccgtg cagtgacttg
    17041 tgtagcttat cctctgaagg acgctctact gtgatcacgc cgtctgcaca ctttattatc
    17101 atatccttgt ggaactcttt tgtgagtgaa cccttaggac cctttactgt aactacgttt
    17161 ccgtctgcaa cagtaacctc tacacctgca ggaacactaa ttggcttaat acctattctt
    17221 gacataactt agtcctcctt accagatgaa tgcgagaact tctccgccga cattcagctc
    17281 tctagccttc ttgtctgtga taacgccctt tgaagtagat acgatagcaa tgccaagacc
    17341 ctttataacc tttggcatat cctcgcagct tgcgtaaatt ctcagaccag gctttgatac
    17401 tcttctgagt cctgtgatga ctggatttct gttctcgtca tatttaagag taattctaat
    17461 gataccctgc ttaccgtctt caatgatctt gaagtcctta acgtatcctt cgtctacgag
    17521 gatctgtgtg atcgacttct tcatattaga tgcaggaacg tcaactgttg cgtgcttcgc
    17581 agtacttgcg ttacggattc ttgtaagaag atccgctatt gtatcagtaa tttgcatgcg
    17641 tgttaacctc cttttaccaa ctagccttct taactcccgg aatcttaccc tcgtgagcca
    17701 actctctgaa acaaatacgg caaacgccga actttctcag ataagcgtga ggtcttccgc
    17761 agatcttaca tctgttgtaa gcacgagtgg aatatttagg cgtagcctgc tgtttgttga
    17821 tcatagcctt cttagccatt ttttatcctc ctcttactta tcagcaaatg gagcacccat
    17881 aagtgaaagc agctcctttg cctcttcatc agtttttgca gttgtgataa agttgatatc
    17941 cataccacga accttatcta tcttatcgta ttcgatctca gggaagatca gctgctcctt
    18001 aagaccagta gagtagttgc ctctgccgtc gaaagagtta ggattgatac ctctgaaatc
    18061 tcttacacga ggaaatgcta cgttgaaaag tctgtcaacg aattcataca ttttctcagc
    18121 tctcagtgtt accttaacac cgatgatctg tccgtctctg agcttgaagt tagcaacgga
    18181 cttctttgcc ttacaaacga tcggcttctg tcctgtgata gcagccagat cagtcatgat
    18241 agcatcgata accttcttgt tatccttatc tgcgccgcag cccacgttga taactacctt
    18301 atcaagcttt gggatctgca taacgttagc gtattcgaat ttcttcatca tagcaggagc
    18361 tacgtcgcta acataatatt ctttaagtct agccatcgta atatcctcct aattaaaatt
    18421 cttcgccgca gtctgcgttc ttgcagactc taactttagt accgtcctca aggaacttat
    18481 gagcgactct tgttggcttg ccgcacttag ggcaaactgc ctgtaccttt gaagcgtaca
    18541 ttgcagcctc agcctcaact atgccgccct gctcgccctg acgtcttggc ttaacgtgct
    18601 ttgtagcaat gttaagaccc tcaacgatta cctttccctc cttaggggaa acttccagga
    18661 ccttgccctt cttacccttg tccttaccgg agaggataac gacggtgtcg cctgttttta
    18721 cgtgtacctt attcattgtt acgacacctc caattaaaga acttctggag caagtgaaag
    18781 gatcttcata taatccttct ctctgagctc cttagctact ggtccaaata tacgagtacc
    18841 tcttgggttc ttgtcttctt tgataataac agctgcgttt tcgtcaaaac ggatatatgt
    18901 gccgtctgca cgacgaagac ccttagctga acgaacgatt actgctttaa caacgtcgcc
    18961 ctttttaaca actccgcctg gtgttgcctt tttaacagaa caaactacaa cgtcaccgat
    19021 gtttgcatac tttctgcgtg ttccgccgag taccctgatg cacataagct cctttgctcc
    19081 ggagttatct gctaccttca ggtaagtctg catttgtatc acagtgcgtt cctcctttca
    19141 gaatttaacg tgcggtcggc agaagttatc tgccacctgc actattgcgt tttaattact
    19201 tagccttctc aagaacgtta acaagacgcc atctcttgtc ctttgacaga ggtcttgttt
    19261 ccataacttc aaccttgtcg cctatattgc aaacattgtt ttcgtcatgt gccttaagct
    19321 taactgttct cttgatgatc ttcttgtaaa gtggatgctg tacgttatcc ttgatagcaa
    19381 ctacgatagt cttatccatc ttgttggaaa ctacaacgcc aactctagtt tttctcaaat
    19441 ttctctcttc gctcacagtt aatcctccct tcgaaaatta ctgaacattg gactcttgct
    19501 tacgaatgaa tgtctttaca cgagcaatat ccttcttcac agcctgaagt ctcttaggat
    19561 tctccaactg atttacagcg tgctggaaac gaaggttaaa aagctcctgc ttgagctcag
    19621 cgagtttgct gttgagctcg tcgactgtca tatccttgat ttcattagcc ttcattattg
    19681 ctcaccaccc gtttcttgag tatctctttt aacgaactta cacttgatag gcagcttgtg
    19741 cattgcaaga cgcattgctt ctctagctgt ctcttcaggt actcctgcga tctcgaacat
    19801 tactctgcct ggcttaacaa cggctaccca atactcaggt gcgcccttac ctttacccat
    19861 tcgagttcca agaggctttc ttgttgcagg cttatctggg aagatcttga tccaaacctg
    19921 accgccacgc ttgatgtatc ttgtcattgc gatacgggca gcttcgatct gatttgatgt
    19981 gatccaagca ggctgaagag cctgaagacc atactcaccg tgagaaactg tgttacctct
    20041 catagccttg cctgtcatac ggccgcggtg ctgttttctg aacttaactc tctttggaag
    20101 cagcattact tgttacctcc ctctttttta gccttattga agtcacgctt accatttctg
    20161 ttctggcgtc ttctgtcgtt tgactctctc ttgtctgaag cagcaacttc gcccttaaga
    20221 acctcgcctc tgtatatcca taccttaacg ccgattctac cgtaagttgt gtgtgcctca
    20281 gctgtaccat agtcgatgtc agctctgatc gtctgaagtg gaattgtacc ctcgtggtat
    20341 gtttctgctc tagcgatctc tgcaccgcca agacggccgc cgcatctaac cttgataccc
    20401 ttagcgccga gcttcattgc tctgccgata gactgcttca ttgctcttct gaatgagatt
    20461 nnnnnnnnnn tgtgcgatgt tttctgcaac gagctgtgca ttgatgtcag gctgctttac
    20521 ctcaacgatg ttgatagcaa cgctcttgcc gatcatcttt tcaacgtttg cacggagctt
    20581 ttcgatctct gcgccgcctc tgcctattac gataccaggc tttgcacagt ggatgtggat
    20641 tcttaccttc tgaccgccgt ccttatcggc aaatctttcg atctctactt ttggaacgcc
    20701 ggcgtatacg cacttatctg ctggattctt tgatcttgta ttcagctctt taagaataaa
    20761 gttacgaacg ttgtaatcct caaccagtgt atcgccaaaa gtaccctttt ctgcaaacca
    20821 gcgggagtcc caattcttaa tcacaccgac acgaagtccg tgtggattta ctttctgtcc
    20881 catagtttac ctcctctttc agcttattcc ttttccttaa gaacgatcgt aacgtgtgat
    20941 gttctcttaa ggattctata tgctctgcct tgtgctcttg gcatgattct cttcattatc
    21001 ggtcctggac aaacatagca ctctgcaacg taaaggttgt tcttatccat gttatggttg
    21061 ttctcagcgt tgtgagcggc ggacttcaga agcttctcaa gatattcact tgcagccttt
    21121 ggtgtatgct ttactgttgc catcgcaaca tcatagtctt ttcctctgat cagatcgaga
    21181 acgatctgta ccttacgagg agcaatacga gcatttctca gaattgcttt tgcttccatt
    21241 tgtattcctc cttccgcact acttaccgtt gtttgaggtc tttgaacctg catgaccctt
    21301 gaatgttctt gttggagcga actctcccag cttgtgacca accatgtcct ctgttacata
    21361 tacaggaacg tgcttacgtc cgtcgtgaac tgcgaatgtg tgaccaacga attcagggaa
    21421 tattgtagaa gctctgctcc atgtcttgag caccttcttc tcgcctgcat cgttcattgc
    21481 cttaactctc ttgtaaagga cctcctgtac gtatggtccc ttcttgacac ttctacccat
    21541 tgatcatctg cctcctttca gcattactta ccgtttctgc gttttacgat aaacttatcg
    21601 gagcgatgat gcttagcacg ggtcttgtat cccattgtag gcttacccca aggtgtaact
    21661 ggagttggtc taccaactgg agcacgaccc tcaccaccac cgtgtgggtg atcgcatggg
    21721 ttcattacag aacctctaac ggtaggtctc cagcccatat gacgctttct acctgcctta
    21781 ccgatattta cgttctcatg gtcgatgttt cctacctgac cgattgtagc catgcagttt
    21841 acagggacct ttctcatctc gcctgaagga agtcttacaa gtgcgtagtt gtcttcctta
    21901 cccatgagct gagccatgtt tcctgctgaa cgaacgagct gtgcgccctt accaggataa
    21961 agctcgatgt tgtggatgaa agtaccaaca ggaatgttga tcattgcaag tgcattacca
    22021 ggcttgatgt ctgcgtctgc gcctgctctg ataacgtcac caaccttaag accataaggt
    22081 gcgatgatgt atctcttctc gccgtcctca tactctacaa gagcaatgaa tgctgaacgg
    22141 tttggatcgt actcgatagt aagaactgtt gcgttcatat cgagtttgtt tctcttaaaa
    22201 tcaattacac ggtattttct tcttactccg ccgcctctgt gacgaacagt gattctaccg
    22261 tagctgtttc tacctgagtt cttcttgagt ggctcaagca gagacttaca tggagcaacc
    22321 tttgaaagct cggaataatc ggtaactgtc atacctcttc tgccggctgt tgtaggcttg
    22381 tagctcttta ttgccatttg aaattcactc cttatctgag ttttgcgaaa aggtcaaagc
    22441 gttttcacgc caagcgccct tccatactta cgtcctgcgg tgaaatgatg agcggtcctc
    22501 gccgcctcgc aagacctttc cgaagacttc tcggtattca tgaagttctt cggagcaatg
    22561 cagccttacg ctgcacctct tttagaggat ttagaacaag ccctcgaaga attcgattgt
    22621 cttgtcaccc tcaagagtaa cgatcgcttt tttccagtct ggtctgtagc ctacgcttct
    22681 tcccattctc ttctgcttac ccttaacgct gatcgtgttt accttagcta cctttacgtt
    22741 gaaaagctct tcaactgcct tagcgatctc gatcttgtta gcgtccttag cgaccttgaa
    22801 agtgtatctg ttagcctgaa gtctgtccat agagtcttca gtgataactg gctttaaaat
    22861 aatatcttga gcagttctca ttatgcgtaa acctcctcaa tcttctttac agcctctgaa
    22921 gaaacgataa acttatcata gttcagaatg tcgtaaacac tgagagtatt tacagtagct
    22981 gtcttaacgc caggaatgtt ggctgcactc ttgacaacag tcttgtcaga ctctgctgta
    23041 acgatgagag ccttaccctc agcaccaagt gccttgagca tctcaacaac tgcctttgtc
    23101 ttgtagctct cgagcttgat tgaatcaaca actacgagat tctcgtcaac gacctttgtt
    23161 gaaagtgcag acttcatacc gagtcttctt tcctttttat taagtgaata gctgtaatct
    23221 cttggcttag gaccgagagc aacaccaccg tgtacccact gtggagctct gatagaaccc
    23281 tgtcttgcgt gacctgttcc cttctgtctc catggctttc taccaccgcc tcttacttct
    23341 gttcttgtaa gagttgactg tgtgccctgt ctctggttag caagataatt tacaaccata
    23401 gcgtgcatga tagccttgtt tggctcgata ccgaaaatag catcgttaag ctcagtgtcg
    23461 gcaacctttt tgcctgtcat atcaaatact gaaatctgtg acattatgat tcctccttcc
    23521 actaagcttt cttaacgcag tcgcaaattg taactgtacc gttctttggt ccaggaattg
    23581 cgcccttgat tgcgataaga ttgttttctg cgtctacctt gacgatctca agattctgta
    23641 cagttacctt tacgttaccg tactgaccag gcataccctt gcccttgaag attcttgaag
    23701 gtgatgaaca agcacccata gaacctgcat gtctgtgtac aggacctgta ccgtgagttt
    23761 ccttaagtct tgagttgttg tgacgcttga tcgtaccgga gaatccgtga cccttgcttg
    23821 taccgctgac atcaacgatg tcgcctgcag caaatgtgtc agcctttacg atatctccaa
    23881 cattaagagc tgatgtgtca gcaagtctga actccttaag agttctcttc attgcaacgt
    23941 cagccttctt gaagtgaccc tgaagaggct tgtttactct cttcgctctg atgtcgccat
    24001 agcccagctg aacagcctca tagccgtcat tctcaactgt cttcttctga acgacaacgc
    24061 aaggaccagc ttcaacaact gttacaggaa ttacttttcc tgcttcatcg aaaatctgtg
    24121 tcataccgat tttcttaccg atgattcctt tttgcattat gttttcctcc tatgtggtta
    24181 ttggtcggac ttgtccgacg tgacctgcgt gttaaatgtt ttttgagatc gcacgcgatc
    24241 tcatttggag attacttgat ctccataaca atgttaacgc ctgcaggaag ttcaagattt
    24301 tcaagagctg ctgttgtttc ctttgtaggt ctcagaacgt cgataagtct cttgtgagtt
    24361 ctcatctcga actgctcacg gctatccttg tacttgtgta cagctctgag gatcgtgatt
    24421 acctctttgt ctgtaggaag tgggatagga cctgaaacct gtgcgcctga acgcttaaca
    24481 gcctcaacga tctttgctgc agctgcgtct acaaccgcat gctcgtaacc cttgatcttg
    24541 attctgatct tttcattgac tgccacttaa atcgcctcct tgtgcaaatt aaaaaagttt
    24601 agggggcagc tttctttcac tcaagcctca gtcaattgcg taaatgatcg aggcatgccg
    24661 atcgcttacg caattgacag gttcgagctt taaaagcttt ctgttgatac ccgtttacac
    24721 tctgccgtgt gttccctgcc attctcataa aaaattccga aacccacaag gctttcggac
    24781 tcagaaaaca gcgcacacag catcacacat actcacgcta aagcagacct tgtccgctat
    24841 cgcaagaagc ataagcacat actgtgttcg gtattaacag tcgcccggtt taaaatcgga
    24901 catactccac ggaaattacc tgaccaactg tcagcaacct tccgcttcat cgcatatcat
    24961 catcgtgtac attaaataca ctgtgcagtc acgcaagtat tattatacac tactttcaag
    25021 ccatttgcaa gcggttacag acaaattaca atatagaatt tttgcctaat tctcaacgtg
    25081 tttatatgca cattgtacaa atttcactat gagtatcatt tgccgctaca ttttattcac
    25141 attcgctgca aaagtcggtt tttcggggct ttagcagggt aaaacgcttc tatttatcgc
    25201 aataattttg ctatcgcaaa aagaacgggc gatcacaggt cgcccttaca agaggtatgg
    25261 tttaagggac ggcgtcctcg acgtcccatt ttgctgtatc atgttataca aagtttatat
    25321 tatgccactt cgtcagtgtc atagtacgcc ttgaccttct cttccgtttc atcggtgatc
    25381 gtctttttga tctctgtttc ctcatcgctg aatataagat cagcaagctc agaggggata
    25441 gtttccctga tcgagccttc atacattgct ccgcctcttg gctcccacat cttggtcatt
    25501 gtccaattgt cgtcctgctt tttaaagtcc aacattctta cgaccagacc gccgctgtac
    25561 atattgcccg cacctttata atgtccgtca ttgctcagta tatagacctt gcaggtctca
    25621 tcgttgatac tctgcacgcc cataagataa tgggattcca caaaaaagtc gtttttgcct
    25681 gcattctcat agtcatttgc ttcgcctcta ttgtatgtgc gcattatcac cgcatttgag
    25741 atatccttgt cagtgagacc ctctggcagt tggtggacga cctccgcatc acagccctta
    25801 taaaaatagt gtcctccaat gccgtgagtt ttgtaataaa acgacacgaa ctttgcccct
    25861 aaaacgacaa ctatcgcagt tatcagcaca aggaatacca acagtttcac atacactttc
    25921 tttttcggct cttcataagc tgcgtttgtt tctttatttg catcgttcat attaagcacc
    25981 cctttctgtt gtctgtatct tctgatttgt tcttacagta acataataac aaaaatctgc
    26041 accaaatgca agcatttgta attttgctaa gatgatttta agatttatca taacaatgca
    26101 caaatttgca cttccgattt tattcactta tacaatgtgc agaaatgctt tgcattcggg
    26161 caaatacaaa aacagagcgg cacatttcat accgctcttt ttcgttattt tgccatatta
    26221 taatgtatta ctgcttaacg tttcccataa tgataccacg tccgtcagtg cctacataaa
    26281 ctctgccgta taccttaggg tcgccgctga ttatctttct aacgttgccc cacttctgtg
    26341 tatcatcatt gatacgcttc catgttacgc ccttgtcggt ggactggtag attccgtagc
    26401 cgtcgccgtt tgcctcgccc atcatataga gtgccatgta gtcgccgtct ttttcagcct
    26461 taccaagacc gatagcgtca cactgtgtaa cgtcctgaga tgtaggagca agcgtgcctg
    26521 ttgcagtatc cagataataa acgccacctg cgccaactga tatccaaagg tcgccctcag
    26581 tatcaggaca agcttttatt gatgttgctg aaacgagcat atttgctata ggactgaaag
    26641 tctttccacc gtctgtactc atatagaatg ttccgtcata tgagccgtag aacttatcag
    26701 gatttacctt atctgtttca ataactgcac cggcaggaag tccctcgcag gtagtccatg
    26761 ttttgccaaa gtcgcttgtt actgccgcag caacgcctgt tgtgccgctc tgatagaaaa
    26821 tggtcttgcc gtcagcggaa agcttagctg agccgcctgc ttttttgcca actccctctg
    26881 ggagagtttc aacagcctgc caggtttcgg ctgcgtctgt tgaatagtaa accgagccgt
    26941 cgccatcgtc tgttgctctt actactatct tataatcctg tgccgcacaa tctatagatg
    27001 tcgccttaaa gtcgccataa tgctcctgcg gagcttttgt tacatcatca tggcggaagc
    27061 cgtaaaggtc acccattgtg gaataaagct caggagtacc ctcgccatta tcgagaggtg
    27121 aaacgaagtc aaagatagct gtcatttcaa tgcccattgc ctttacgctt atgtcaacag
    27181 gctcgtcctt tatctttgaa agatttgatg tgccgaaaat agttgcacct gttgtgtaaa
    27241 gaacttcatc tgggttgaac gggttgatag caacacctgt catccaccag cccggcttga
    27301 gctgtccctg ccaatccagc caaggagcat tgcttatatc gagattaaag ttgtttacct
    27361 ctttgccgtt ctcgtcactg ccccaaatgc cgttccaagt ttcgcctccg tcataggaaa
    27421 cgaaaaggtt gtcgaagtat gcccaaaggt caagagttgt gcatactatc atgttagggt
    27481 cgttagggtt tactgagata ccattgtaac cgcaagtata agatctctca ggagttatct
    27541 ctgtccactc gcctgtttta aggttatact tctgaactgc tccgtcagaa gcaccgtttg
    27601 gtcctacctt ataagaccat gttgaataga gatatccgtc agaagatatc tcgccctgac
    27661 aaggcttgag cttctcggtc ttgtcgcctg tagcctcaga ctttggatta gggatagctg
    27721 tccaggtttc gcctgcgtca tcagaacgat agagataatc cccttgtgtt cctgctgagc
    27781 caacgattat tgtctttgtt gcctgaccct tttcagagct tgatttatcg aatgccacga
    27841 atgtaagtcc gatagaatat ccgtcttcag tatatccgcc ctttgtaggg aatgaagtga
    27901 cctcgttcca tgttgcgcca tagtctgtgg acttccaaag tccgtcagct cttgagccga
    27961 agtaaagtat agagttatcg ttagggtcta cctgaagtct ttcaccacag cctcttccga
    28021 cctcgttacc gcccatgcca aaaggcattt cgcagatagt ccagttcttg ccgtaatcgt
    28081 cagaagcaaa tattgcaccg ttgtttgtgg agtatgtacc agccgcaaga taaactctgt
    28141 ttggctcaac agggtcagtt gcaaggctct ctgttccgtt atagttccaa tcgtcgccgc
    28201 caaagcaatc tgttatacac tcccagcgct gagtatcctt attatactta taagcgccac
    28261 ccatatctgt tctggcgtag ataagtccct cctcagtagg gttatagatg acgtcaggga
    28321 taaaaccacc gtcaactatc tcgacatttc cccactccca gccagtgtca acaggctgag
    28381 cgttgtaata ctccttctta gcgtcgccct tgctgtcaga gccgctggag gaatcgttct
    28441 tttttgagca gcctgcgaaa gctgtcactg taagggcggc ggcacaagcc actgccatta
    28501 gcttttttaa cctcataaaa attctcctta taagttgtat tcgtttacat ctctgtgtta
    28561 tttaaaggcg gaagatctcc gcctttaaaa tttgaactta ttttacaact attgtactac
    28621 aatatcttca aattgtcaat agtcttttgt gtatttatct aaacttacca caactttgat
    28681 aaacgctttg ctcttacacc tttataaaat gaagaatctt tgagcagaag tcgccataaa
    28741 gtccgtcacc attccacatc tttgcgatgt tgatacctgc attcataagt gccgcacctg
    28801 atatcttctt gtcaggctcg ttctcctcat aatagtaagc gtcagcttca agacctttga
    28861 gcttgatacg tcttgatctt tcgtttggtc tgccaagcac ctgaacgaat tcaaagagag
    28921 cttcgctctt gtcctttgca acgaactccc aagcgtccca ggtgttgtcc tcaaacatat
    28981 tgcctattct gtagtggtcg cctgttctta caagcttgtt gaacttgttg aactcctcta
    29041 tctgagctgg gatagcgtcc ctgtcctcct gcggtatcct tgttacgtca agctcatagc
    29101 cgaacgtacc caccatagca acgtgacctc ttgtcttgaa aggagtgttt ctgccaacag
    29161 tatgattagg gcagtcagaa acgtgcgcac ccattgctaa gcatggatag cacattgaag
    29221 tgccgtgctg tatcttcagt ctttctatag cgtctgtatc gtcagagcac catatctgtg
    29281 ggctgtagta aagcatacca ggatcaaatc ttgctccgcc gcctgagcag ttctccagca
    29341 ggatatgagg ataatctgta gtaagtctgt ccataaggtc ataaacaccg agaacatatc
    29401 tgtgccaaag ctctctctgt ctttcagcag gcagggtagc cgagccgacc tctgtaagct
    29461 gtctgttcat atcccacttg atgtactcga tattcgcact gtcgagaatc ttcttcatca
    29521 tgccgtaaac atgatctctg acttcttttc ttgaatagtc aagaacatac tgctctctgc
    29581 aaagtgtcag aggtctgccc tttatctgga tagcccagtc tgggtgagcc ttataaagtt
    29641 cagagtcagg ggatatcatt tcaggctcga accaaatgcc gaacttcatg ccaagcttat
    29701 ttacctcgtc aacaagatat ttaagaccgc ccttgagctt cttctcataa acgaaccaat
    29761 cgcccagtga tgagttatca gaatctctat gaccgaacca gccgtcgtcc ataacaagca
    29821 tttctatgcc aagctttgaa gcttccttag cgatatctat aagcttgtca gtatcgaaat
    29881 taaagtaagt agcttcccag ttgttaatga gtatcggacg tcttttatcc ttatactcgc
    29941 ctctgataag gttgtttcta tagaggtcat ggaaagttct tgacattttg cctataccct
    30001 cgtcagagtg caccattact acctcagggc aggtgaagct ttcgcccttt tccaaaagcc
    30061 actcgaagtc aagaggattt atacccataa tgaaacgagt tttcttatgc tgagtaactt
    30121 ctgcctgagc atagaagttt ccagagtaaa caaagttgaa accgaaaacc tcgccattat
    30181 tctcgtcagc gttattgtcg caaagagcaa cgaacggatt gttctgatgt gatgattcgc
    30241 ccctgcagga atcaacaagc tgtttaccat ggtgaagtct gcatctttca acagctcttt
    30301 ctctagccca cgagccgttg agggttatca tatccatttt gtctgtatca aaatcaacgc
    30361 aagctgaaag ggctcttgtt atcttgaacg gcttttcgct cttgtttgtg ataacagcgg
    30421 aacgtgttat aacgtcaagc ttatcgaaag ctgtgtagat aagaacgact tcgatatcag
    30481 catacttatc atacattgtt atctcgagag ttgaacaacc gctttcctca gtagcgaaag
    30541 tagcaggcag accctcaagc tttggcttgc cctcatacat tttatgagaa acatatctca
    30601 ggtcgcaggt cgacataccg tcagcgtcca ttattttgaa agcagactct ctatagtcac
    30661 caagaccgaa gcatggatat tcaaaaggca gtgtatccat aaagcttgct ctgtcacggt
    30721 cgttagtggc aggtgtgaaa gggctttcgt caagacgtag aagataagtc agatcctcgt
    30781 caggaacttt atttccatag taaacgtgag cgaggtagcc ctcctcgcag accttcatca
    30841 tatagtctgt attattggct ctcagcttga aagccttatc tttttcgtta taaaagatat
    30901 tgataattat cactccttgc aaaaatctga accttacggc tcattgatat tataacacat
    30961 agaaaaaata atgcaacagg gtaaaacgat tactttgaaa attattttgt taatttttct
    31021 tgataataca aatatttgaa catgggtggt tcaaattgtg ggaatatata aaaaagggac
    31081 ttatgcattt agcacaagtc ccttaaaaat tcaatattta tctgttatca gattagttgc
    31141 cgtacttagc agtgctgatc ttgataccag ggcccattgt agaagctaca actacgctct
    31201 taaggtactg acccttagca gcggctggct tagcctttac aacagcgttg ataagtgtgt
    31261 tgaagttctg ctcgagcttc tcaacaccga atgaagcctt accgataggg cagtgaatga
    31321 tgtttgtctt atcaagacga tactcgatct taccagcctt agcctctgta acagccttag
    31381 ctacgtctgg agtaacagtt cctgcctttg ggtttggcat aagtcctcta ggtccgagga
    31441 tcttaccaag acgtcctaca acacccatca tgtcaggtga agcaactaca acgtcgaaat
    31501 ccatccagtt ttcctttgtg atcttctcaa cgagatccat gccgccaaca tagtcagcgc
    31561 ctgctgcctt agcagcctcg tacttgtctt ccttacagaa tacgcaaact cttacagtct
    31621 taccagttcc gtttggaagt acaacagcac ctcttacctg ctgatcagcg tgtcttgagt
    31681 caacgcccag acgaatgtga agctcgatag tttcatcgaa ctttgcctta gcacccttag
    31741 ctgcaagttc cagagcctca ggagcgtcat attgcttagc cttgtcgata gccttgaagc
    31801 tgtcaacata cttcttgcca tgtttcatta atatttcctc ctcattaagt ggtaataccg
    31861 acagtggata tctagtcggt tagcggaaaa ttcctcccac cattgccgtg catgaatgca
    31921 cagtacgatt gattagtcct caactacaac gcccattgaa cggcatgtac cagcgatcat
    31981 gctcatagca ctctcgatgt ttgctgcgtt caggtcaggc atcttctgct cagcgatctt
    32041 ctgaagctgc tcttttgtga tagttgcgac cttagtcttg ttaggaacac cggaacctga
    32101 ctcgatcttg caagccttct tgatgagaac tgctgccgga ggagtcttgg tgatgaatgt
    32161 gaatgatctg tctgcgtaaa ctgtgataac aacaggaatg ataagaccga tatcgttctt
    32221 agttctctcg ttaaaatcct ttgtgaacgc catgatgttt acaccgtgct gaccaagagc
    32281 tggaccaacc ggtggtgcag gagttgcctt tcctgctgga atctgaagct taatatagcc
    32341 tactaccttc tgtgccattt gttcgcacct ccataattaa gtggtaaggc gagcgccata
    32401 cgctctccca cgtttccgga agccgtatat ccgaaccgtc attgacggct ttgcttccat
    32461 tttcacgaat gtttgcggag ttttaccgcc tttttcctta ttcctcagac tttatctgtg
    32521 tcagcggcat agtaacagtt gtttctctgc ccagcataga tactctgacg tctgccgtct
    32581 gtgcttccat atcgaatttc tccacaactc ctgtgaagcc ctcgaatgaa cctgctgtta
    32641 caacgacgtt gtcgccttcc ttgaagccga cctgaacgtt agcgcttctc tgtgtgtcaa
    32701 cacccagcgc tgcaacctct ttatcggtca gcggaacagg ctttgacgca ggaccaacga
    32761 agcccgtaac gcctctggta tttctaacga tataccatga atcgtctgta agcaccattt
    32821 ttacaagcac atagcttgga aaaagctttc tgtcgccctt gtcgatctta ttgcccttta
    32881 cttcggtaac ttcctccatt gggatcatta cctcagggat cagatcctga aggtttcggt
    32941 tttcaacaac cttttcaata ttgcttttta ccttattctc ataacctgaa taagtgtgaa
    33001 caacatacca ctttgcttct tctgacatgt ttcttacctc catattgatg ttgtcaaaac
    33061 cgctgtgacg gcttagccgc cgatcttaag gatagccttg atagctgcac ccagacctga
    33121 atcaacgccg aaaaggaaca gagccataac gaccattacc gagagaacaa ctcccgtatt
    33181 cttagtcacc tgctgtcttg taggccaaac gacctttttc atttcagctt taagctctct
    33241 gaaatatttt cttattccgc cctttttctt ctgatccttg ccggtatcct tttttgaaga
    33301 cttagtaaca tctgtttttt tagccattag aaatagctcc tttcaatatt acttagtctc
    33361 tttgtgaaga gtgtgtttct tacagaactt gcaatacttg ttcatttcaa gtctgtcagg
    33421 gtcattcttc ttgttcttct tagtgttgta gtttctctgc ttacactcag tacaagcaag
    33481 tgtaattttt actctcattt cggcacctcc tgacgaaatt tataccccgc aagaacattc
    33541 agggtacgtc tgcctccctc atacgttttc acgcaagctg tcacacagcc ctgaggtaac
    33601 ttatgctttt tcacgctcaa aaaaagagcg caaaaaaata gacctcataa agaggtacta
    33661 taattataac atctgtgctc ttctttgtca atcatttgtc aaagaatttt attaattttt
    33721 tgtaaatttt atcgtttgtc gagtgaggaa tgaggagtga ggagtgagga atgaggaatt
    33781 atggtatcgc ctgtcggcga tgattttctg taggggcgaa cactgttcgc ccgtgttggt
    33841 cgcccgctca acttcatgaa aacccaaaac tcgcaactcc ccgaaacggg ctgtcgagga
    33901 cgccagcccc tacacttatg cgctactttg caatgccgca aaatcgctgt cgctctttgc
    33961 ggactttact ctgtataagg atatagtata agcggtgcag ttttgttctt cgatacaact
    34021 ttttattaag ttgcaacgtt gcaattcttc ttttcctctc tgcaccgcct aatcggcttg
    34081 cgccgaatga ggctctgcct caaactccgc aatgggcttt gccccttgac cccacaagcc
    34141 ctctaacgcc gggcttgagc ctgcgtttta tctctcgcta cgctcggtgc tgggggcaca
    34201 gctctggtca gctatgctca ttcctaattc ctcattcctc attaaaccgg aaactttccg
    34261 tcctccacgg tcgcatcagc gatctctctc atctcgtccc agtcatgcga gtaatggtca
    34321 ttcaagcaag ccacagcaaa gaacccgcct gctttcgctc cttttatgcc ctcgactata
    34381 tcctcataaa ccacgcaatt cttcgattca agccccagtt tctcagccgc cagctcataa
    34441 acatcaggaa agccttttcc cctctccacc tgctctgtag acacgaagca atcaaattga
    34501 tccaaaatgc cgtttctctt tagcacaggc ttataaagct cctcagttga agcggtcgcc
    34561 aatgctatct tcctgccctg ttccttaagc tttcgcacat actcccctgc gtttccgcaa
    34621 agctcgataa cgttcgtata ctcatacacc gccatatcga accattcctt cattatattc
    34681 tgaatgttct cacccagaga aaatctatcg tttgtataaa cagccgcctg ctcgaaattc
    34741 attgcagaca cctgcttgaa atagtcctca ggcactttta tcccacgctt gccgagaaat
    34801 tcctcgtcta tctttgacca aacgtgattt gatcttgtca gcgtgccgtc caagtcgaaa
    34861 atgtgtccct taaaatcttt tatgtccatt gtaatgtcct ttcagaagta ttcatcagag
    34921 agcctgaagc cctcgcctat tatctcgctc acgtcaccca caagcacgaa agccttttcg
    34981 tcagtttccg atgctatcct gcgaacgtcc gttatcctgc gttttctcac tgcgcaaagt
    35041 ataagatctc tgtcctctcc cgtgtatccc gttcgggctt ttatgaccgt acaccccaag
    35101 ttgccctcat taagaagcct atggagtatt ttatctgcgg cagggcttat tatcagcgca
    35161 agctttcctc tgtcgccgcc gtagagtatc atatccagtg tttttgagaa cacccacagc
    35221 gtaaagaacg aatacagcgc gttgtcgata ctgccaaaaa caaaaccgct gagcacacat
    35281 atcacaccgt ctgcaagcat aaatatctgt cctgccgcca tgtagggctt tttccttttg
    35341 ataagctttg cggcgatatc agttccgcct gttgttcccc ctgaacgaaa aactatccca
    35401 cagcctgcgc ccaacagaat accacctgct attgcgctga ggaaaatatc atttgtgagt
    35461 ggtgtaaaaa gcgtacacat atccgctgtc agacccgaaa ctgcaatagc tacaacagtt
    35521 cccacaagga aacgccagcc gaacacataa aggctaaccg ccaacaaagg aagatttatc
    35581 gccattgtaa gcgagccgac accaagaggc agtaatcttg acaacatgac agcaagcccc
    35641 gaagcacctc cgggggcaat ttcgttaggg cttaaaacaa gtcccacgct cacgccatat
    35701 atgaccgctc cgagaagcaa gagaggatag tccactctgg cctttgcgga cttttctgcc
    35761 gaaaacaatt tattcagaag tatcataaaa gccctccaag ctatgcaaaa gcaaagatag
    35821 gcttattttt cccctgatac ctcgtaaaat acatttttac aaaagaggcg caatggtcct
    35881 gagaatgacc gacatgaacc ttgaccaaaa gctgcggtta tttatatcgt catgagttat
    35941 cctatgacag cggttttcaa acatatcgtc aaagtccgct tttatatcct ttatacaact
    36001 tgttttgtac atataagttg cacactcaaa atggagatac aagcttctgt agtcgagatt
    36061 tatcgtaccc accacggccg acttatcatc cgagaggaac attttggcgt ggttaaagcc
    36121 gcctttatac tcatagactt tcacacccag cgtttcaaga tctctgtaga aagaccttgt
    36181 tataacgctc acataccatt tatcaggtat ctcaggagtg attatcctaa catcaacacc
    36241 gctttttgcc gcatagccca ggacggtgag catttcgttg tcaggaataa gatacggggt
    36301 agagatatag atataatcct tggcattgtt tatcataccc atatagacaa gctcgcccac
    36361 attctcgtca tcgagagggg agtcaccgta ggggataaca tagccgttgt tcacaacgtt
    36421 ttttacagtt gggcggtaca tattatactc agttttatca cctgagatga cctcccacat
    36481 ttgcagaaac atcatggtaa aattccaaac gccgtcgccc tttagcataa ctgcggtatc
    36541 tttccaatga ccgaaacgct ccttgcggtt tatatactcg tcggcgatat taacgccccc
    36601 gttgaaagcc acgttgccgt cgataacaag aatttttcta tggtcacgat tattctgtat
    36661 agtagaaagc atagggcgga aaggattgaa aacaaggcat tttacccctt ggtcggtaag
    36721 ctttttctta tatctgaaag gtaaagtgaa ctgagagccc atgccgtcat aaagaagtct
    36781 tacctccacg cctgccttag ctttttcaag aagtatctta actatctcac gccacatttc
    36841 accgtcgtcg ataataaaat actccataaa gataaactcc tgagctttct gaagctcctc
    36901 aagcatagcc ttatacttat cttcgccaag gggaaaataa gtgacctccg tattaccgca
    36961 gacagggtag ccccccgact tatccacata gtttgcaagc ttataaaaat caggctcact
    37021 ttcatggaga tagctcatta tctgttcatt tgttttaagg aaaggtcttg tattggcgga
    37081 tttcttagca taaaggtttc tgacctttct tgttgaaaac tgatttttga gtaatatata
    37141 aagcaccacc gtaaagatag gcacgataac aaggggtact atccacgcca atttataggc
    37201 agggttctcg ctgctgttaa ggatatagat agcaagcacg atagccacag tagaaaaaac
    37261 gatatgcaga taggcataat gcacagcgaa gctttggaag atgataaaaa tgaaaataag
    37321 ctgcaatgca agaagcagga taatagtggt tttctgtgag aatatcatat ttataagttt
    37381 tttcggtttt ttcttttgta ttttttcgct tatcttttcc gccacattat ccgcctgcct
    37441 ttctgaaaat agtgatacac aactgattat aacatttatt tttgattttt gcaatacgca
    37501 aaaatgcagt taatccgccc tgatttcacg aatttctgtg acaatgacac aacttttgtt
    37561 atcttattgt tgacaacaat ttgcgaaaca acgcacgaac aatgcagggg cgaacagtgt
    37621 tcgcccgttt agttcaccca catggtttat ggaagctcaa acctcgcaac tccccgaagc
    37681 gggacgtcga gggcgccgtc ccctacaatt gcgctatggg atagaaacaa agcggtgcag
    37741 agtttttgct ccgacaccgc tttgttttgt tctttacctt tatatgcttt ttaacttttt
    37801 tctgcaccgc ttgtaggcta cgcctattga ggctctgcct caagctctga cagggctttg
    37861 ccctgtaccc acaaggggtt tcaccccttg accctgacca ggggcgtgcc cctggtcctt
    37921 ttgctcgcta cgctcggttc tgaattttac ttcatgcttg gtctttactc tggtcagcta
    37981 tgcccattcc tcattcctca ctcctcactt ataaccctca cttaatgcgt tctacaatat
    38041 actcctcgcc gctcaccgct ctatgcaaag cgtccagcag tccgtcttcc tgccaagtga
    38101 gatcagttct atcaaacact ccgccgtcgt cccacaagaa tgccggtatg ccaatgtcat
    38161 gtgtgagctt cgtaaagtac tcgtcaaaaa acactctgtc cacctcgtca ttgcctttgc
    38221 agtttccata ctctcccaca ataacaggta tgccattatc cacaaatgta gttttaagct
    38281 tattcacgtt attgaccatt gtttttatct caggctcagt tccccagtta tactgatttg
    38341 tgcaaacgca gaactcccaa ggcgtataat aatgcaccga aactatgcac ctattttcag
    38401 ggtcgtcagg cattttgaaa agctttgagc aagtcttttc cacgtccgtc caatagcctg
    38461 ctatcaaaag atgccttttc ggattgtttc cgccctgttt tcttataagg tcaacaaagt
    38521 cctgatttat cttgttgagc agttcgtaag cctgatttgt tcccatatcg tcaaagccca
    38581 cctcattcat ggactcgaaa ataagcttat cagaatagtt ctcaaacctc tttgctatct
    38641 gcgtccagat cttgttatac ttttttgaaa agttttcgta atcagcctcg gcttccgttc
    38701 ttatccatgc accgaagtca gggtcaccgc caccgtcatg atgaacgttt atgataacat
    38761 agaaatctct gtcataagca taatcaacga cttcttcaac tctgttcatc cagtcctcgt
    38821 ctatggtgta atcgtcagac aggtggtcac gccatgtgat aggtattctc actgcgttga
    38881 agccgtcctt tttgagctgg tcgaaaattt cgggagtagt tttcacattt ccccacaggg
    38941 tttcgtcaac tttctctgaa ttttcgtcca cctgaccgtc accgtcacgg tcggcgttgc
    39001 acacgtcaag ggagttgccc aaattccagc acaaattcat ttctttcaca aggtccatag
    39061 aagaaatatc acgcatctcg cctttttccg taacgtggga gcttccgaca ttttcagcct
    39121 ttgaagagct gtctgcattt ccgcaggaag cgaacactcc cgccgccatt acaacggcag
    39181 aagccgccgc aaatattctt ttcattttta tcatatatat cacctttccg ctatctgcca
    39241 tacatgttca tattttcata taagatcaaa tatacaaaga cattttccac ttatgtatct
    39301 ttacacaagt cattatagca ccaaaatata aataaattga aaatgaacag gggataattt
    39361 ctttatttgc gtattaattc aaattcttgt atacactcac aaatcgaagt catcttattt
    39421 gtacataacg ccacataaat acacaaaaaa acaggcagac ggctttccag agcaagccgt
    39481 tcccacagcc gcttaaaaaa accatctgcc ttattatttt tgctaaaatt tcaaacagct
    39541 attcagtata gccaaaggca agcacttctc catgcttatc ctcatcgcct gccgccattg
    39601 atatttcaac ggtatagtta gttgcttcag gcatcatggc gatattctgt atctgagcat
    39661 ttccccagcc tgacggactt atggtatcgt aatcgctttc gtcatacacc tctccgtctg
    39721 ctgttacctt aacatgaagc ttgccgaact cgccctgctt cacctctttg tatatcatgt
    39781 acaagaattt tttgtccttg aactcgaata cgatagggct gttatctccg gtgttgtcgt
    39841 aagtccagcc atttgtgaag cttgcaatag tagagccttc cttccaactt ccaaggcttg
    39901 ttggcgtaag gctgtcgccc tcatacatat gagcgtccac ttctcgtgga gagaaaacag
    39961 tgtctgtggg cataacataa tcgccctctg gcgcaacgtc cataacagtg tcaaaatagt
    40021 agtccaccat tgaagccaca agcttgtgac cctctttgtt agggtgtgac tgatcgtcag
    40081 agaaatcttt ccaagtcatt ctgttgtttg cgaaaagata tcttagtgcg tcacagtatg
    40141 atatcatcgg caggtgataa aactcgccta tctgcttcat ataatcctga gcagaatggt
    40201 cgttttctgt caccgagaac agcagaacag gagtgatatt cttctgcaaa agcgttctga
    40261 ctatggactc gtaagcgttc tgatagtcag tctctgtgcc gtcatttact gcaaattcga
    40321 taaagcatat gtcaggctca taagccagca catctctgtc gagcctgagt attccaagct
    40381 tagacggcgt accgctaaga cctgcgttca catactggac gttatccccc gtaccgaatt
    40441 tctgtgcaaa ataatcataa gtgagcttag cataacagcc gtctgcaccg gcagaaatac
    40501 cctcagtgat agagccgcca agatacgcca cagtgacctt ttcgcctgac tgtgctttct
    40561 ttataacatt ctgcatcata gaagtgttgc cgtatgacac catagccttg tcatacatag
    40621 cctgcgtcca ctcctcgtca gtttcaggca ttttttcgac ctgcgagctt gaagactccg
    40681 ctttgctttc agcctttgag ctgtccgcat tatttccgca gcctgcaaaa gccgctccgc
    40741 acatagcaac actcacaagc actgccaaca ttttttttgt attcattatt tgtacctttc
    40801 taatctatga taacgaaatc ggtttcacac cgtatataac acaaaaagta cggacaaagc
    40861 cgtacttttt ggttatatca atatgataaa aattcttatt agttaagaac tgccttctca
    40921 gtttccttat cgaagatatg gattctgtta ggatcgatag cgaccttaag ctcatcctgt
    40981 ggacgaacgt cagaacgagg tgaaactcta gctgtaagct ggataccctc gcagttgagg
    41041 tacagatatg tttcagcacc catcatttca gttacttcaa cagttgtgtt gataacgcct
    41101 gttgtagcct gagcgaggaa tgcaggctca tcgtggatac actctggacg aacaccaaga
    41161 acaacttcct gatcaaccag tggctcaaga acttcaggat ctaccttaga ctctggaact
    41221 gttacctgat acttaacgcc tcttgttgtc tttgtatcct cagaaccgaa ctcaacaaca
    41281 tacttgccgt ctaccttgag aagcttagcg tcgatgaagt tcatctgtgg tgaaccaatg
    41341 aatcctgcaa cgaactggtt tactggatcg ttgtaaagat ttgttggtgt gtcgatctgc
    41401 tgaatgtaac cgtccttcat aactacgata cggtcaccca ttgtcatagc ctctgtctgg
    41461 tcatgagtta catagatgaa tgtagtaccg agcttcttat ggagcttaga gatctcagtt
    41521 ctcatctgtg ctctcagttt agcatcgagg tttgaaagag gctcgtcaag aaggaatacc
    41581 tgtggctcac gaacgatagc tctaccgagg gctacacgct gtctctgacc accggagaga
    41641 gcctttggct ttctgtcaag aaggtgagcg atgtcaagta ttcttgctgc ttcctctacc
    41701 tttcttgcga tctcatcctt tggtaccttt ctgagcttca gaccgaatgc catgttctca
    41761 aatactgtca tgtgaggata cagagcgtag ttctggaata ccattgcgat atctctatcc
    41821 ttaggagcga tgtcgtttac aagtctgtcg ccgatgtaaa gctcaccttc actgatctct
    41881 tcaagacctg cgatcattct aagtgttgta gacttaccac aaccagaagg acctacgagg
    41941 atgataaact ccttatcctt gatctcaatg ttgaagtctg aaacagcgat aacgccgcct
    42001 gcatacttct tataaatttc ccttaatgat acacttgcca ttaaaaaagg acctccctat
    42061 ttgaatttta ctaatatcct aattagctat gcttttatca taacaaattt ttaacatacc
    42121 cgcaagagtt ttttttacta aactttatta tatctcatta tgaatattga cgaaaattca
    42181 catagaatag gtaattactt acatttaaac gatttcgctg tcaataatct ttcaacatct
    42241 ttgtgcatta tttctaaaca gcttccaaga ggcaggtccg gatctaaagg cagttctccg
    42301 atctttatcc gcttaagaaa aacaacttta tttcccagct tttcaaacat tctcttgacc
    42361 tgatgatact taccctcatg aagcacaaca gtacattcat cgggacagtt cacacttgcg
    42421 gaaaattccg caggcagaca tttctctccg ccgtcgatag tcatgccctc agcgaatgcc
    42481 tgagcataat tttcctgcca agggtcacga agtctgacga aataggtttt cggaacatgg
    42541 gtcttaggtg acaacattct gtgggcgaga gctccgtcat cagtgagaag cacaaagccc
    42601 tcagtatcct tatcgagcct gcccgcaggg aaaagcccct cccggaacat ctcaggaggt
    42661 actagctcaa ggacagtttt cgactctccg tcacgagtgg agcaaaccac accctgcggc
    42721 ttgttcatca ttatgtagac aaacttttta taacatatct ccacgccctt tacggcaaca
    42781 atatcgtttt cgctgacctt agcatcagcc gccttggcga cctttccgtt tacagtcacc
    42841 tgaccttttc tgcaaagctc tctcaccatg cttcgggaaa tatcattacg ctgtgaagaa
    42901 acaaatttat caagtctcat agcaaaagcc tttctgacaa ttttcttata aagattatag
    42961 catacgatac cgccaaaagt caaatatgcg aaaaaacttg accaaaagca aacgcctgca
    43021 gatatgctcc acgggcgttt tattactatt attatcggtt ttttcgacct atgaagcatt
    43081 ggtttaccct tattcatagt gccgacgttc cacgctcaga cgcctacttt ttcctcagcc
    43141 cctgcataaa tccgtccagc tccgctgagc atacatctcc tcccacaagc tgtccctcgc
    43201 agacgataag attagccctg acctcctgct tcttattccc ataattcttc acagcatatg
    43261 tatacacctc agcggttttc cctttatagt cctccaagtc aaagccctgc tgtttctgaa
    43321 gcttattata cttttcatac acctcattga attctttcgg tatggtcacc gccttgcatt
    43381 catcgtattc gtcagacacc tcccagccca tttctttcaa aaacgcctgc ctttgcgctt
    43441 cgcttttcat ctcgtacacc gcaggcgacg atatcctcac cactgtcact attatcaccg
    43501 ccaacagtgc caacgccgcc agaaccgccc cgatcaccgc aaatgttggc ttttttactt
    43561 ttattgtctt gataaacata cccttttcct cgctttctgt tttcaaagtt tgtacattta
    43621 tatgtattgt cccaaaaatt ataactacct gtccgatttt ttattttgtt atataatttt
    43681 caaaaataat ttttatagca ttaataatct tattgtataa taaatttatc ggtttgctat
    43741 gatttattat tacaannnnn nnnnnatatg gcattgtgca aaattgacaa aaataaaagt
    43801 gaaaattaat gtgaattaac gattttgtat ttttttgtac aaatgtcttt gacaatttca
    43861 aaatatgtgg tacaatatta ttaggtaacg ctatgcttct ccgcattgcc tgcggagggt
    43921 actgaaacgt tttcttaaat ctgccttttc acggctgtga aggggcaagt tattattgct
    43981 gatgcgttct gcggtcagcc gcaggattga tatatgaaag gagcaatgag ttttatgtca
    44041 aaaattattg cagttacttc cggtaaaggc ggaacaggca aatcttctat aagcgcctgt
    44101 ctcggttatg cccttgccaa gcagggaaac cgtacactta tcatcgagct tgactttggt
    44161 ctgagatgta tggatatcat gcttggtatg cagggtaata ttacatacga tctaggtgac
    44221 gttcttgagg gtacttgcga cgtatacaag gcaacaacaa ctgttaagct tgcttcaaac
    44281 ctgagcgttc tctgtgctcc gtccgacccg ttcgttcagc tcaaggctga ggatatcgaa
    44341 aagatcacac aggaaatgag aaagtatttt gaatacataa tcattgacac ctctgcaggt
    44401 atcaacggtt ctgtatttga tatagttact aattccgacc ttatacttat agttacaact
    44461 cctgatcctg tatgcgtaag agatgcgaga atgatgtcag atgagttcta taaaagagga
    44521 aatcagaagc aaagacttgt tatcaacaag gcaaacaaga gaatattcga gtttgacgaa
    44581 atggacgacc ttgacgctat catcgacaca gtcggcgttc agcttctggg tgttatccct
    44641 gaggacagtg ctatccctct tgcaacagga aagggtgcgc cgctttcatc aagctctatg
    44701 ggcttccttg cattctctgc tatttcaaga agaataaagg gcgagcatat acccctctcc
    44761 atcaaggtct gagtatatta tcagccgtat gcgtgagggt atctgcgcat agtgcggctg
    44821 ttttatataa cgaaacttat ttgttcatta atatactaat atataaacgg ggggtttcca
    44881 ttgaatattg cacttattgc tcatgacaca aaaaaggaac tcatggtaca gttctgtatc
    44941 gcctattgcg gtgtactcag caagcacaat ctttgtgcca caggtactac gggaaaaatg
    45001 gtagccgaag caacaggcct taacgttcag cgttatctca gtggctcaca gggcggcgac
    45061 cagcagatcg cagcgagaat atcctgcaac gagatagacc tgcttctgtt tttcagagat
    45121 cctatctcag tgaagccaca cgaacctaat gatatgaatc tcctcagact ttgcgatgta
    45181 cacaacattc cggtagccac aaacattgcc actgccgaag cgcttatcca tgcacttgag
    45241 cgaggcgatc ttgactggag agatatcgtt aatcctacaa agatataatt gaaatttcat
    45301 atacaaaagc gatgaaaggg agagtattcc gttcttatgt ttcagcgaga gccgttggtg
    45361 agagggttca cacaaagggc aggaagaagg acaccttgga gcttccgttt gttcaagaac
    45421 ggacgtatag ctgcgttaaa gcgttagagt gatccggcac gaaagtgttc ggataattag
    45481 ggtggtatca cggtaaaagt cgtcccttac taggggacgg ctttattttt tttatccacc
    45541 acccgtaggg gagcggctca atttctgctt cacagaaatg cgacgtcccg ctttttcgct
    45601 tctcacttat tctcgaatac cttatctcag cacaccaaaa agcaaaggcg gtataacaat
    45661 gaaagccagc cagctaacaa aagaaaacat ctcccccgaa atagtagaca agcttctttt
    45721 tgagggtctt gactataaag gcgaaactgc cgacgttata atagttctgg gaagccgcaa
    45781 agcctgcgac tatcgtgttc cccttgcggc aaagcttttt caggagggaa aagccccaca
    45841 tctcatattc tgcggaggga aggtgcaaaa aacctcgctg ggcgaaatgg aagagtataa
    45901 agccatgctc atcgccgccg aaaaatgcgg cataccgacc tccgccataa ctgtggaaac
    45961 acactctctt gataccgtgg aaaatctgca aaacgcagcc aagcttgtaa aaaagcattt
    46021 tccaaactgc aaaagcatta ttatagtcac taccgcatat catatgcgcc gcgcaaaaaa
    46081 gcttgcagag cactttttca cacagaaaat tatcccctgc cctgccagcc gtggcagcac
    46141 tcagcgaaat aattggtcaa tgaccgaaaa aggcagcaca acagcacttg acgaatgtct
    46201 taaattcggt tattatataa aaaaaggttt tattgacgat tttgaaattt gaaacgttgt
    46261 aattacagca ccaaggctct ttaatgtagt ggtgaacagt gttcgcccgt ttattaacaa
    46321 cgttccgata tcacagctat ttaaacgata tcggtattca aaagttcaag acaaaataca
    46381 gaaaggatcg ttattatatg gcattgatag ttaaaaagcc aaaaggcaca gaagacattg
    46441 ttccctcaaa ggtctataag tggcacactg tagagaagat agtttccgaa gctgccgaaa
    46501 tatacggctt caaagaaata agagtaccaa cttttgagga aacaggtctg ttcgtgcgtt
    46561 ctgttggcga aacaacagac gttgttcaga aagagatgta ttcagtttcc gcagcaggcg
    46621 actcaaagtt cgcactccgc ccggagggaa catccggcac aatgagagct gtccttgaaa
    46681 acggcattat gaacgagggt ctgccgcaga aggtctatta tatcctctca tgtttcagac
    46741 acgagaacac acaaaagggc agactcagag agttccacca gttcggctgt gaaatggtcg
    46801 gctcagaaag cccaagagca gacgcagagg taatatctct tgcaaaaagc gttctggaca
    46861 gagcaggact cagaaacatc aagctcaaca tcaactctat aggctgtcca acctgccgtg
    46921 cagaatatcg caaggctctg aaagagtatt ttgccgcaag aaaagaccag ctttgcgaga
    46981 cttgtcggga cagacttgac aaaaatccaa tgagaatact tgattgtaaa gtcaaacact
    47041 gtcaggagct tggcaaggac gcccctgtta tcctcgatta tctctgcgat gattgcaaag
    47101 aacactttga gaagcttcag gagtacctct ccgtaatggg tatcgacttt gacatcaacc
    47161 ctaagatagt aagaggtctt gactactata caagaacagt tttcgagttc atcaccgatg
    47221 agataggcgc acagggcaca gtctgcggcg gcggacgtta tgacggtctt atccagcagc
    47281 ttggcggcaa gccaacccct gccctcggct tcggtcttgg tcttgaaaga cttctcatgg
    47341 taatggacgc tcagggctgc gactatatgg agccaaagac ctgcgacctt tatatcgtac
    47401 caatgggcga ggacgccctt aaaaaggcaa tgggcatagc ctccgagctt agagaagagg
    47461 gctgtttcgt agaatatgac cttataggca aaggacttaa tgctcagatg aagtatgcaa
    47521 acaaaacagg cgcaaagttc gttatggtgc ttggcgacaa cgagatagaa tcaggcgttg
    47581 caaagctcaa aaacatggca acaggcgagc agacagatat caggcttgac aacaccattg
    47641 ccgagaactt ttcaaacgca cttatggcgg atatgttcaa ggatatcgac gctgatatag
    47701 agaaatttat cggaaaggag aactaagcaa tgagatgtga acagtgcgag aaaatacctc
    47761 tggtagaaat acacactccg caggaatata tgctctgcat aggttctctt gcaagaatgc
    47821 tgctggcagg ggaagttgaa atgacctatc agacctgccc acttgataag ataatggata
    47881 acgacaacaa gtggtatgct gaaaagcttt tccaccagtt caggtgtaca aaatgcggca
    47941 ctatctatgg tatgctctgc aacacccaca caggcggaca gataaagata aacgacaagg
    48001 tctttgaccc tgctgattat cccgataaaa aggaagataa ataatgcttg caataagacc
    48061 cgtgacctat gaggtggaga cctcactttc gcccaaaaga gttctcagaa agcttgacgg
    48121 cgaccttatc gagcatagac ccaccataaa cattctctcc caaagcaagt tcatgaaaaa
    48181 ccaccgtgac gataccattt tttatggcta ccgcacagac gccgaaagct ttcagatatt
    48241 ccaccatacc gctaaaaaac gtgacggagg ctcaacaggc ttttatggca aggtagaaaa
    48301 gacccccaac ggctctagga tatatggcaa gttcagaaag cctatctatg cctatgttgt
    48361 gggcgtcata tggacggtgc ttgtgctttt cctcaccctt atgcttgtgg cactggaaga
    48421 gaaaacaggc gcagtatgct ttgccgcaat atggctcgtg ggattgttcc tcatgttctg
    48481 ggacaacaag aagaagttcg tcagagccta tctcgacagc ttcccgcctg ccgtcagcaa
    48541 aacagacgaa agcgaaaaat aatattcagg atttattcac aatctcatca caatatatac
    48601 actgtatatt agtatactaa gagtgtaaaa gaatttatac aacttgttga aagtgcactc
    48661 ctagtagtag ttgccccacc ataaggggtt ttacgaggct aggagttttt tattttcaga
    48721 aaggatcttc aaaaatgaaa gctgatgata taattactgt aaagcgtgaa atatcaactg
    48781 caatattagt tgacggcggc ttttatcgca aaagagcata ttacttttgg ggtgaaaaat
    48841 cgccaaagga acgagctgat gaactatacg attattgtat gaagctcttg cataatcagc
    48901 atgaacagcg atatttatat cgtatttttt actatgattg cccacctgtt gaaaaaaatg
    48961 tttataatcc acttacaaag agaagcgttg accttgcaaa atctgatgaa tacaagtgga
    49021 ctaacgaatt tttcaaggaa cttaaaagca aaagaaaggt tgcactaaga atgggacggc
    49081 tttctgaaaa ttcacttcat tacaacataa agccagaagc tttcaaaaaa cttgtaaatg
    49141 gacagataac aataagtgac cttacagaca atgaccttaa acttaatttg gaacaaaagg
    49201 gcgttgatat gcgtgttgga attgacatat cttctctttc gttcaaaaag caggttgacc
    49261 agataatact tatagctggc gacagcgact ttgttcctgc cgcaaagcaa tcacgccgtg
    49321 aaggtataga ttttatactt gcgcctatgg gtgcaacaat taaagccgat ctatatgaac
    49381 atattgacgg ccttatcaac agaccaacaa aaaaaacaga aaaggaagta atgtaaaatg
    49441 aaacttgata caatgaaggg cttacgcagg acccattact gcggcgaagt gcctgaaacc
    49501 gagcaggaag tcgttgtatg cggctttgcc gacagagtaa gagatatggg caaccttatc
    49561 ttcatcaatc tccgtgacag aactggactt gtacagcttg cattcaatga tgaaacagac
    49621 agagccgttt ttgaaaaggc tcagcttgta aaaggcgagt atgtcctcat ggcaaagggt
    49681 atggtacgtc ctcgtgagag cgtaaacgaa aagctaaaaa caggcaagat agaagttgcc
    49741 gtaacagacc tgagaattct ctcaaaggct gaaacaacgc catttgaggt aacaaactca
    49801 gataaggtaa acgatgagct gaagctcaaa taccgctacc ttgaccttag aaatccaaag
    49861 cttcagaaga atatcatcac ccgtcataag atcgctcaga taacaagaga gtatttctat
    49921 gacaatggtt tccttgaaat agaaacacct atgatgatga agtccacccc tgagggcgca
    49981 agagattacc tcgttccttc aagagttcac gcaggcaagt tctatgcact tccacagtca
    50041 ccacagatat acaagcagct ccttatgata tcaggttatg acaggtatat ccagcttgca
    50101 agatgtttcc gtgacgagga tctcagagct gacagacagc ctgagttcac acagatagac
    50161 cttgagatgt cctttgtcga tcaagaggat attcaggctt gtattgaggg ctatatcgaa
    50221 aagctattca aagagatact cggtgtagag gtaacgctcc cactgccaag acttacattt
    50281 gcagacgcta tgagccgtta tggctctgac aagcctgata cacgtttcgg catggagata
    50341 caggatatca cagacaccgt taaggatatc gactttgtgg tattcaagtc agctatcgag
    50401 gcaggcggct cagttcgtgc aataaacgtc aagggcggtg cggcaacata cacaagaaaa
    50461 gagatcgaca agctcgttga gcacgcaaag ggcataggtg caaagggtct tgcgtatatc
    50521 cgctgggcag acgagccaaa ctgctcattc aagaagttcc ttggcgaggg cgatcttgag
    50581 aagataaacg cagccgttgg agcagaaaag ggcgacctcg ttcttatctg tgccgacaag
    50641 aacaaggtag ttcttccaac tttgggcgca ctcagactta tctgcggaaa gcgtcttggc
    50701 gttatcccag agggcaagtt caattttgta tggataacag aaatgccatt ctttgagtat
    50761 gatgacgaga acgacacatg ggtagcagcg caccacccat tcacaatgcc aatggaggag
    50821 tgtcttgact atcttgacac cgaccctgca aacgtccgtg caaaggcgtg ggatcttgtc
    50881 ctcaacggca cagagctttc atcaggctca atgagaataa cagactttga gcttcagcag
    50941 aaaatgtttg aagcactggg catgacagac gaagagatag aagcaaagtt cggcttcctc
    51001 gtagacgcct atcgttatgc cgcaccaccg catggcggca tgggcatagg acttgacaga
    51061 ctttccatga taatctgcaa tgcagacagt ctgagagatg taatagcgtt cccaaaggta
    51121 cagaacgcca gcgagcttat gagtgcttgt ccgtccacag tagacgagaa gcagcttgag
    51181 gagcttcaca tcaagaccac agaaatagaa gaataattcg cattaaccaa agtagatcct
    51241 ttattacagt tatgaatcac aaagggcaga tacttcatta tcgaggtatc tgccctttac
    51301 tttataataa cgtataaaac catatagcac accatttttg ttaataataa cttgtttttt
    51361 tgactaaatc atgttacaat atgtatatgt tgttattaat tcagaacaga ggtggcaaag
    51421 tatgagcaaa aacaataatc cgttatataa agctctgcag gatcatactc gaaaacggat
    51481 ctcactggag gagctaaacc gaatatgtca agatcatgaa cagttagccc aacagatcag
    51541 cgagcttact gagcagggca ttttgactcc tgtcaaaagc agtggaacaa atggcaatct
    51601 gaaaatacca ctgtatatga gatatacgat ctctatagaa aagccacagc aaatatcacg
    51661 ttatgagatc gatgctctcc atccaaggct gacaaaaaat gaatatcttc ttcgcaatcg
    51721 aatacagttt gtaaaaaata aggctttttt acttcgatta agtgaatggc tgacgtatga
    51781 caagggcagt gatatgatgt cccgacgtga acgttccttt tctatatttg gtgacgagaa
    51841 aaaattagac gaccatttaa gactattaga aagaatgggc atcactggcg atgtgcttaa
    51901 attttacgaa acaccggaac agtgcttttc cgattatatt cccgttcgta aaaacagaat
    51961 gacattgctt atttgcgaaa acaaggatat ctggttcaat ataagacgct taatgtatag
    52021 ctccggagcc tgctcgatat ttgatactaa gatcgacgga gtgatctttg gtcaggggaa
    52081 tgacattaca ggaaaagaaa aatttcgttc ctacgcacaa tatttaggag ccgatgaaac
    52141 aagatttctg tattgcggag atattgacag agctggtttt gacattttta ttcgtctttg
    52201 caaggaagca aaagagcttc atattgaatt gttcgttcct gcatatcaga aaatgctgga
    52261 actgagtcga agcataaggc tccctgacag cagcgacgga agacgcatca ttcccgaaat
    52321 gtcagggata ttgcccttgt tttctgatta tgagcaaaag cagatcacac aaataatcga
    52381 ggataataaa cgattgccac aggagatact ctcatatccc gtactggcag ataatatgag
    52441 gtaaaataat gatctcagca ggttccaaag aattattaca agattttgaa aaacgtatgc
    52501 gattcgtgaa tctcatcaaa tactggctac aaagaagcaa acccacagag atccgtgaaa
    52561 tattgcataa tgatgatgat aaaaccgata atctcatact tatggtaatg gtctttatca
    52621 tggacaatac acttcgctat ggcgaacgct gtaccaagca ggatatcaca gtatttctgc
    52681 gtgaattagc cgatatttat ggatatgagc cggaggaggc aaagaccctg acagattata
    52741 tcgtaacaga tgtactgcaa agcggaggaa aagtgcgaag atttgaaacg ttcgatagtc
    52801 atgacgaaaa attccatgaa cagggctctc tgatcttatt cgagcagcaa agcggaagct
    52861 atgttctgac aaatgaagcc tatgagtttc ttttcagaac caaagagatc gacagcgaat
    52921 tggacttttc tgtaagccga ttcaagttac aagagtttat taagcgtggc aactatggca
    52981 aagcacttcg tgaaagccgt gaattagtta gcagagtcag aaatctgaaa acaagaatgg
    53041 acgacttcat gcttcggtgt aggatcaata tctctgaggt atcgatcgat gaatacgaag
    53101 atatcgtcga tcaggtcaga gattcctttg agaacgaaaa caaacagctc agcgatatcc
    53161 gcagccttgt atcggcaaaa ttgcaggcat tcgcagtcgc tgaaataaaa gaagaccgcc
    53221 aaatatgtca gaccgaaaaa gagatacatg agatccttga aaacattgat actgtaataa
    53281 gcgagcaaag ccgagtattt aaccagaaat tcacactatc tgaactatat gctgatcttt
    53341 tagatgatag cttctcttat ttgcaggcgg gtcactttga cattgcacag gagtt
//
LOCUS       DAWEMR010000003        51749 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_296_length_51749_cov_10.546814, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000003 DAWEMR010000000
VERSION     DAWEMR010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 51749)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 51749)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..51749
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_296_length_51749_cov_10.546814"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2105..2114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15411..15420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22910..22919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    43762..43771
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        15135 a        10202 c        11905 g        14467 t
ORIGIN      
        1 caatgagcga cagcgaatat agctgtcctt attacaggct tgatgatgaa tatgctgttg
       61 tgagaaagca aaattgatga tagatcggtg aatacatgaa atttgccgat tttttcttgc
      121 tttgttgaaa gtataaagtt caaaagcttg tcaatataga cgtgtaaagt ttatttgctt
      181 tgcagtgaac atttgtcgtt aaataaagac cgatctgggg caggattaat atttggcgaa
      241 tgatagaaag ttatttatca aagtttacaa aatattaata aaaacaggaa agattttttg
      301 ggggaaagtt gcatttagcg taaaaacgca ggtttcatat tgtttcatgt tcaaggctaa
      361 taaatatgcc gttttatcat ctgccggcac tgtgaaaatg ataaatcaat attgtgcgaa
      421 ctatcagtat atttatatgt aataaaatac aaatatgata tattgtggga ttgacaaatg
      481 gaaaatcaag tgatataata cttgtaatta tataggcata ctgcgtcgga ggtgaggctt
      541 ttgaaggata aggcgcttga aaaattgtca aaaaatgagc tgctcgagtt aatgctttat
      601 ctgcgaaaag aatatgataa gcttaacgca gagaatgaaa agcttcttca aaagctcgaa
      661 gataaaaaaa gtatcactga cagacttgac aggctggagc ggcaggtagc tgcactgaca
      721 ggtcacaagc tggttgttga gcagccggta gcggaggaag atcatggcgg cagatagcaa
      781 gaacgaggtg ttaaaaaaaa atgagcttcc tgatgtccag acacttgagc gcgtggtaaa
      841 aaagcgtcat gaaaggcggg aatatctgag gatactccgc agcaccgtgg cgtcgcttct
      901 tgttgtggca gcggcagcag tgatcatatc aatgttattt ttgcctgttt tgagagtaac
      961 gggtacaagt atgacgccga ctttgcatga cgacgagctg gtggtgtgca gaaagcgtgg
     1021 aagctttcag aagggcgata tagttgcgtt ctattataat aataagatac tgttgaaacg
     1081 tgttatcgct acagcaggag atgtggtgga tatcaaggag gacggtactg ttatcgtcaa
     1141 cggaaaaacc ttgaatgagc cgtatgttga tggcaaagct ctgggagaat gtgatatcga
     1201 gttgccttat caggtgcctg acgagagaat attcgttatg ggcgaccaca gatctacgtc
     1261 tgttgacagc cgtacaaaga ggataggctg tattgctgag gaagcagtgc ttggaaaggt
     1321 ctcactgagg atatatcctt ttaaaaggat aggcacagtg gactagcgtt tattataaat
     1381 agtgtataat ttcggaggga gtgatccgtt ttgcagaatt ttaacggtaa agttcggcaa
     1441 tatctaaaag aaaacgaaag gaaaaaacgc agccgcttgt tcggtgtggt gctgtctgta
     1501 gttatgacag tatcagtgct ggcaagtctt gttatgcctg caataagtgc cactgagggt
     1561 acttccgtgg caggtggaga tgtttcctat aacggcggaa actattctta taaggatgcg
     1621 aatagcgtca cgataaaatc ctatgatgcg gtcggttcag gaaaagacca atctaaaaag
     1681 atcgaattca atgtggaatt tgattttgac aagggcgcaa tgaccaatag gtatatttat
     1741 ttccctatcg acgataatgt tagcctgccc gagggcggta taccgtcaga cggaacctgg
     1801 ggcgatgtcg aggatactca cttcgatgaa ggacatggaa tttcaggtaa gtacagagtc
     1861 gacgagatag atggaaagaa gtatcttttt attgagtttg acgagcgata tcaacacaaa
     1921 aatgagaagg acgctataag cggtaaggca agctttgagg gtaatgtaaa gcgtaaggat
     1981 acagatacag gtgataagac cactgttgag ataggcggtc agacggttga aatagacggt
     2041 tttactcctc tcactatgtc tgctataaag gacgcaagcg aaactgctga cggagttagg
     2101 tggannnnnn nnnntgacaa tcctgcaaag aagcctcttg acaggatcga ggacgagctt
     2161 tttaaggatg ctgtagacgg cagttttgtt gtttcacctg agggtgcagg aagctatgac
     2221 gctgcaagcg gaaagtttgt tttcagcgat ggattcagcg aagaaaatgt taccataagc
     2281 tacacgactc catatcctac aagcgacccg aatttcgtta tgtcaggtaa ggtggaaaac
     2341 aaatccacca catatgataa ggacggaaat ggagtaaaag cagataaaac tatttattct
     2401 gagtcaaaga aggataagat ctcaaaaaca ggcaagaatg ttgactacga caataacgaa
     2461 gtcacatggg aaattgttgt aagcaaccct agcggtgcag acctcaaggg ctatcacctt
     2521 tatgatgaag cattccctga tgcaaagaca ggaagctttg cacttaaagc tgatgatggt
     2581 tcagatgtaa aatacacact tgacggaaac acgcttacgt ttgacgataa aacaacagcg
     2641 tcaaaaatta ctatcgagta tgtgacagct atcgatcctg acaaggcgga aactacaaat
     2701 acagttaaaa tgcaaagacc tgataaaagc caaccatggt ctgatatcac atcaagtgaa
     2761 actgtttata ataggtatca gatatcaaaa agtggttgga tagataataa cacgttaggt
     2821 atactcaaat gggaagtaac tgttaaatgt aatgacaaaa attctaccct taaaggcaag
     2881 acaataactg ataatatgct taaagcagga cagattgtca gcataaaggt tggaaatgat
     2941 acctttgatg caacagttgc aagtgacggt gagcttgttt tgcctgacag aataggtgac
     3001 aataacgagg tagttatttc ctatgaaaca aaggttgccg actatgatct tggcgaccct
     3061 gacagcaatg gaaattatgc agtgaaaaat actgcaggta ttgacggatt ccattctgac
     3121 aagacagttt attataagcc tgtaaacgag aagtcaaaaa cagttcttgg catttctcag
     3181 gacggaagca gtgttacata taagtggcag gtagaggtca agcagacaaa cggttccttt
     3241 aggggcaaga ctataagcga tataatgaac gctacgtcaa atgatggcaa gtcaataaag
     3301 agcgttcttg acactgattc cattatcatg tatgttcaga gaaatggaac aggaagctat
     3361 gaaccgcttg acagttcaaa ttacactgta gtacctaatg acgacaacac ttcgtttgag
     3421 ataaagttca atgatagtga agaatttgac aatataaacc ttgtgcagat acagtatagc
     3481 agtaccgtag atgtaacagg tcttgacgaa ggtactatta taaatgtaaa taacaaggct
     3541 acttataaag gtgaaatatt cgagcctagt gcagataaga ctaagtttac tataggcgac
     3601 agctccaaag cgccttatga aaagtatgac gctgcaagca gtgtagcagg cgacacagtg
     3661 catgatctta gcagcctgcc tgttgttact gtaaatggtg ttgagtatta caggtttgat
     3721 tggcggcttg atatcaacaa ggacggcact tataagcgta acgataatgt cgagatcgtg
     3781 gacacgcttc cggcgggaat gatattctac gagaacaagg attatctcaa gtattcattt
     3841 ggaaatagtt ctgataagta tgattttgca caaacaaatc agtttgaata tttctatgat
     3901 aagacagcca atacagtaac tttcaagact gcatggaatc agcaggacgc ttggacagct
     3961 tactacagta cacttgtaaa ggtcgatgat gttaagaatg gtgtttacaa gaatggtact
     4021 gcagcgttca aaaacacagt tcaggataaa gctggaaaat atccggagag aagccagacg
     4081 cagacagtta aggaaaagaa cttgaagaaa acttcttctc cgaagtccac cggctgtgag
     4141 ataacttata ccattgatgt taaccctaaa aagctcaaat gctcaacaaa cggaatgata
     4201 actctcaacg atgttatcaa gtgcggaggc acggctgtag ttaagacaaa ggacgctcaa
     4261 ggcaacattc agacaaaaac ggtaagcata aatggcaatt ccgctgttga tatggatctg
     4321 aagtctatca aggtgtttga cgcagataca ggctcagagc ttgctgtgag tgattacagt
     4381 tatgtgatat cagatccgga ggcagttgac agcacagttt cctgcagtgc agatgttatt
     4441 accaacgaaa aggccaagtt tatcgctaac tttattccaa gtgaaagtat tgttggcgct
     4501 aagggtacta ttgatctgac tttgcctgag ggctacgaaa acggaaatat aaagagatac
     4561 tatgtggctt atggctatat ggacggcaac agtttcaagg aagtaaaggc ttttgccaat
     4621 aagagcggcg agtggtcaga ttatggctca agcatatccg ttcctgtgga agaggtttct
     4681 tctgagaagg cgggcggtgt tttcagagtt gaatatgaat acagggatag caacggcagt
     4741 tcaagcactg tggtaagcaa tgaaaaaatg ctttcggcgg taaagtgttc cgcattgaca
     4801 gcaaagacac gagattttgt aaagaaactt gaaataaacg ttccggatca gcgtcacctg
     4861 aaaatagtat acgtttatac aggaagtcct gcaaatgatg aggacgctaa gaacgatggc
     4921 tataaagtcc atgtgttcaa taatatatca tttgacacaa acggctctgc tgtaaatgac
     4981 agtgaagata atgattccga gtttgagttg ataaatcagt caaaggcgtc atcaacaact
     5041 gttgagccat ttaagcttat gaaggtagac agcggagact atagtattat gcttaaggct
     5101 tcttttgaga tctacagata tactaagctg gaaaatggca catacgattg gctcccggca
     5161 gtagatttca aagaaaatgc tgcgaaggac agaagatcag ttatatgggg aaattcggca
     5221 caagataagg cgcagacatt caatgtaaat gctgacgggt catacaacct tgatcttgaa
     5281 cctgccgcag gcacaggaga tactgagggg catctttata agcttataga aaagcaaagt
     5341 ccggagggtt atcttcttga cgaaactcct atatattttg cttttaaagt gcgtccggct
     5401 gtagttccaa gtgaagtcgg tgaggacgga tatcagttcg tgaagaacgg agatatgttc
     5461 agagtaacaa atctgctgaa agagtttaag ataactgcaa gcaaggtttg gagcgatgga
     5521 aacggcaatc attcggcaga gagcgtaaag ttccttcttg gctcgtctac aactaaggtt
     5581 ttaagcggtg ttccagatga tattcagtgg ggcagtgatg aggtaactct taacgaagct
     5641 aataattgga aacatgagtg gtcaaagctt cctggatata cgaaggtcgg caatgattac
     5701 aagcagcttt actactatat caaagagatc gatcttgcaa caggttatga ggctgtatac
     5761 agtgcaacgg caatgagcaa aaacactgat gttacagtta cgaacgtcaa gggacttaac
     5821 attacaaagg aatggcgtga tgagcttgga aacaagcttg ctgacagcga gcttcctgcc
     5881 ggtattgaca agatcgaggc agatgtttat gagtcctctg ttgcaccaaa ttcaacttca
     5941 agagtgaatg gagataacgg aataccaaca gattgcaaac ttataaaaac tgtttctctg
     6001 gaaaagtcta agaattggac ggtaactctt gacggtcttg acaaggacaa gtattactat
     6061 gttcttgaaa agaatatacc tgacggcttt ggcgttacat atacttattc aaataacggc
     6121 agtgccacgg gctttgatac gatcgtcaat accaagaccc cgcagaaggt agaaaatatg
     6181 gacgttcgcc tgcaaaaggc atggtctgac ggcgatagca atacccatac caatgataag
     6241 gtaacgttca atatctatag gtcaacaaat aaggctgatg tgcctgaaaa atataacaag
     6301 tttacaggca caatggataa gggacagaat gttaaattca agatccttac aagcaatggt
     6361 gcagaaatgg ctcagaacgg aagctttgcc tctgagtata ccatgtatgg ctcggctaag
     6421 ttcagttttg atatgacaaa gtcatggata aataacggtg aaactgaggg acgtgtcaga
     6481 gttcttgaca gttcaggagc tgttctggct gacatctata agtcaggtga aaattgggtg
     6541 gacggcaagg ctatacctat atggagcggt gatagcaagt atgtcagcgt tagtgattac
     6601 gttacaccgt cttgggacaa gagtcagttt gttttaaatc tgaataatat cacttcagat
     6661 attactgttg aacttacact taagggcgat agtggctgtt catacgcagt aacagctaat
     6721 gatcttggca cacctgagaa gacgaacgtt acacctgttc ctagtaaaga tgcgacacct
     6781 agcggtgaag tattcttgtg gaagcagata acgctcacgg ctgataacaa ctggtcaaat
     6841 gccgtttctc ttgaaaaaaa tgacatcaag ggtagtaagt attactattg gtttgaggag
     6901 acagacgtga acggtcagcc gataatcaca tcgtcataca aagcttacta cagatacaat
     6961 ggctcagaaa atgagaattg cattgatatg acaaagaacg gtgatcagta tgttcaggtc
     7021 tataacaact ctgaggaaac cttgggagag ctgcctgaaa caggcggcat gggtgttatc
     7081 ccattcgtgg cagcaggcgg aacagtcact gttgctgcaa cattactttt agttacaaag
     7141 cgtcgcaaaa aaacgctgta aataaaccaa tattaaagga taattaatta tgaagatgaa
     7201 gaaattcgca gctcttgctg ctgcaacagt tatgtcagtt tcagctattg ctgcaacatc
     7261 agcattcgga gtatttgcta ctactgatgc tggcgttaat aacattgtta tcaaggattc
     7321 tgatacaaac agagtatata aggcttatca gatctttgac caggcatctg caacaaatac
     7381 tatcaagtgg ggcaaaggca ttaatggcga tgctctgctt accaaactga aaacttctga
     7441 ccttaacact tatatgtcac agtttgacag tgccgagagt gcagcagacg ttgcaaagat
     7501 aatctcagat gcagatcatt ggacaaagga agatactgcc aagtttgcaa aagctgcttc
     7561 tagctgcatc atcgacaata agtctgtaac cgcctatgaa taaaacggtg agtacactat
     7621 tccacttcca aatgctggct attatctcgt tgttgatgca acagaaaata atggtgttga
     7681 taaggctaac tcagctctta tccttaacgt ttcaggtaca acaaacgtta ctccaaagaa
     7741 gacgaagccg actcttacaa agcagatcaa gcacaatgag aatgactcat ggggcgatgt
     7801 tggcgacaac gcaatcggtg acgatgtaga gttcaagatc acaacaacta taccaagcga
     7861 cgtatcagct tatgacaagt atacatatac agttcgtgac cagctcagcg agggctttac
     7921 attcaatgat aatcttacat acaagtacta tgacgctgac ggtcaggaga tcacttctgt
     7981 tacagtaggt cctaacaaaa cagttggcga cgacagcagt acgaccgact ataaggagtc
     8041 tttctatgtt actttcgata tcaaggaact tgtaaagaat tatcctacag ttgctaagat
     8101 cgaaacttat tattctgcta agctcaatga aaaagctaag gtagcagtta ctgctcctga
     8161 cagcgtgaac aataacccta acacagctta tctgacttat tcaaacaatc ctcaggacac
     8221 aacaggtaag gagaacggtg aaacacctaa ggtcacagtt tatgactgga cattctcact
     8281 tgttggtaac aaggtagacg aaaagggcgc gcctcttgca ggtgctaagt tccagcttag
     8341 aaatgttcat aacgatgcta tcaggcttgt caagatagct gacaatgttt acagacttga
     8401 gatcggcagt gaggcaggct ctgttgatta tatcgaaaca gacgcaacag gtaagtttac
     8461 tatcaagggc attgatgatc agacaactta caagcttgtt gagatagagg ctcctgctca
     8521 gtataacaga gctgatccat atgagttcaa gtttgacaca acatatgatc ctgctaatac
     8581 acttcagtcc attcgtatgc tcgacggcac tgattcaggc acaagcggtt caacagttaa
     8641 gatcataaac cagaagggca gctcacttcc aacaactggc ggtatcggta ctcagatctt
     8701 ctacggtgta ggcggagcta tcgtactggg cgcaggtgtg cttctcattg ctaagaagag
     8761 agctaagaac gactaatttt tgaatttatt tttacctttg tcctgccgat ctcggtcggc
     8821 aggcatcagg gaggactcca tgaggaagaa aagcggtaaa tcaactacaa ttttattgat
     8881 cttgttattg cttgtggggc tctccgtgat gttatatcca acaataagcg attggtggaa
     8941 ttcaaaaacg caaagccgtg cggtggcgac ttatcagaaa gcggcaaatg acctttctga
     9001 caaggagaat gaagagatac tcagccaagc cagaaaatat aatgaagaga tagcaaagct
     9061 tgcccgacct tttgttgatt atgataaggt caagggctat gaggatatcc tggatatcac
     9121 ggggacgggt gttatgggct atatttccat tccgactatt agggtggagt tgcctattta
     9181 tcatggcaca agtgacggcg tgttaaatgt cgctgtggga catctgcagg gttctagcct
     9241 gcctgttgga ggagcggaca ctcattcggt catttcagca catagaggtc tgccgtcggc
     9301 aaagcttttc agcgaccttg ataagcttgc agagggcgat gagttcacta taacagtgtt
     9361 gaaagaggtc tatacatatg aggtcgaggc tgtttatata gttctgccta atgaaatgga
     9421 taagctgaac attattccag gtgaggatca tgttacgttg actacctgta caccatacgg
     9481 agtcaattct cacaggcttc ttgttcgtgc aaagagagtt gaaactgcgg acgatacaaa
     9541 gagtgtttca aaaataacgg cagacgctgt tcagctcgat tctcttgtgg tcgtgccttt
     9601 tgtagcaata ccgttgtttg tggtactgct tgtgtggtgg tttgtggatt caaaacgcaa
     9661 aaaaatctct catgatgagc ttgtggcaat gctggataag ccagagagtg acgataatgt
     9721 tgaaaatcta cctgataagg agagtagcaa agatgaaact taaattttct cagcttgcaa
     9781 aggctgtttt gtgtacggcg gcaatggctg tttcagcctt gggaagcatg acgatctcag
     9841 cttctgcagc ggataatatc aatgtggata tagtgtgcaa aaatgataca acgactgtta
     9901 gtaatgccga gtggagcata tataaggtcg gtgagcgtaa agaagctgac tttgtcctta
     9961 ccggtgagtt cagcgattat cctatagata tgtctgattt tactgacgct tcaaaaatgc
    10021 aggctgtcgc tgatacgctt gacaactacg caaaaactga tggcataaca ccagtttcaa
    10081 caggcaagac ggacgcaaac ggtgaagtaa agctttctgc tgattctgtg ggtctgtatc
    10141 ttgtttcagg caagagtttt gaaaacacaa cagctaagtt tacaccttct ccgtcactta
    10201 tcgagataga taaggatatc gtggctgagg gtcagataac agtttacacc aagttcacat
    10261 acaaagagat cccagcaggg gacagtgatt atggtgtaag aaagtcgtgg atagttcctg
    10321 atggtcagga aaagctcata cctgataata tcaaggtgga gatatatcgt gatggcaagc
    10381 ttttcgatac tgttactctt gacgctgaca acaattggga gtatttttgg aaagacgata
    10441 ctgcggctga gtggacagtt aaggagatag atattcctgc caactacaca gttgttacca
    10501 aaagcaatga caagctgttt cttatagaga acacatataa gtcagacagt tcgagtaata
    10561 cagatagtag ttcaagcaca cctgacagca gttcgtcagg ttctgattca tcaagctcaa
    10621 cgcctgacaa tataccacag actggttcgc tcaactggcc ggtgcctgta cttataggcg
    10681 gtggagttat ccttatcgcg gcaggttgga gagtaagcag aaaagagaaa taaaggcgaa
    10741 accacatagg ctcgccttta caacaggaga aaaatagtga ctaaaaaaaa gctaggactt
    10801 ttgttgataa taatggggat aatgctgata gcggcggcat tatcccttaa ttattacaac
    10861 tattttcatg aaaagcaaag taataaaagg atggaggctg tgctgagtga cctgaaaacg
    10921 cagatctcag acagttcaga ggacagcgac agcagttcac cctttgacat ttttgacgat
    10981 agtcgatcca cggacagcga gatagatgac ccggataagg atatcgtgct tgacggcaac
    11041 tcttatatag gtttgatttc attcccgaca ctggggcagg agttccctgt gactcgtggc
    11101 tggagctatg ctgcaatgaa tactgcagct tgtcagtaca gcggcagacg tgtagacaat
    11161 gatcttataa tctgcgctca caattacaca ggcttttttg ataagctgga taagctcaac
    11221 tcaggtgacg aggtcatttt caccgatgtt tacggcagag aatttaacta tactgtcaca
    11281 aacagcgaac tgctgagcgg ctgggattcg ccatcgctca taaaaggtgg tggcagcgac
    11341 tgggatctga ccttgttcac ctgcacatgg agcggttaca gcagagtgac tgtaagactt
    11401 gtttattcat aaatacatca aaaacgcaca gagttctaca ccctgtgcgt tttatattcc
    11461 tgcggcaagt cttatttttt ttatatcaaa agtttttagt ttgtcatcgt atatgcagtg
    11521 tcgtcttgac tttcttgggt ggcgacgtgt atagtcaatg gcttcttcag ggttgtttgt
    11581 ttgaaaaact ggacgtttca ttgctttgtt gtatactgtg tatctcataa aaatacctcc
    11641 caaataattg ttataattaa ttataacatt aattggtacg ttggtcaata gcaatattgt
    11701 acaaatttaa cctattaatt agatatacat aaattagaac agaaatcgac ttgtttaaga
    11761 acacttcacg acataagacg acataattct acttataaaa attatagtga tagtgggata
    11821 ttgtgcaaaa atgagaatag ttggtcaatt tgacgaacga taaagtataa atttgttttg
    11881 acagcgaaag ttgtaacaca gttttcattg attgataatt aattgaacat tcaaatatgg
    11941 tataataccc taaagaatcg gagaggagcg aggagttatg gatagctttg tgcgaaaata
    12001 tggaagatat gtaccctttg ctttaactat tatcataata ttttgctgtg ctctttttct
    12061 gaaagaccat gagttttctg agatactcag ttattgcccg gacaatttgt ggcttgccgc
    12121 atttgttata ctgggatttt atggattgaa gtctttgtcg gtggtattcc cgcttgcggc
    12181 tatttttgtg tgcgtgggtg caatatatcc gttctgggtg ggcattttgt tgaatgtaat
    12241 aggactatcg gtgtgtttta ctgttcctta tcttgtggga agaatatctg gtggcgatat
    12301 catgcgtgtt attgagatga aatatcctaa ggcacgaaag cttgtaaatt atgggcatga
    12361 caataatctg ttcgcctcct acatttcaag ggctgtggta gttgtgccgg gcgaccttgt
    12421 ttcaatgata cacggcgcac tgcgaatgcc ttacagacca tatcttcttg gctcgataat
    12481 gggagtgatg cctgaaatgc ttgtgcagac atatattgga gcacagttga agcatttgac
    12541 aataaaatca gtgcttgtga tgatagcact catagctgca acgctggctt tttcactgat
    12601 acttaataaa aaggtcagca ggagcggcaa gcagatggac aaggaagatt tttgatatat
    12661 gtacaaaaaa gcggactgtt gttatcagtc cgcttttttt attatttgtc aaagctagga
    12721 ttgaaagcgt agaaattttt gctgtcaagc atatcctgaa taagtcttag tccttcgccg
    12781 aagcgctggt agtggactat ctcacgttca cgaagaaact tgatagggtc acggacatcg
    12841 ggatcgtcag caagcctgag aatgttgtcg taggtcaggc gggctttttg ctcagcagca
    12901 aggtcttcgt gtatatcagc aatagcgtca ccggtacttt ggaagatttc cgcagaaaat
    12961 ggtacaccag aggcggctat agggtaaacg cctgtagtgt ggtcaacaaa ataggtgtca
    13021 aagccactgt cttttatctc cttgagggag aggtcgcggg tcagctgata gataatagtg
    13081 ccgatcattt cgatgtgggc aagttcttct gtgccgatat cgttgagtat tgcctgcacc
    13141 tcacggcagg gggctgagta acgctgattg agatagcgaa gcgctgcacc aagctcgccg
    13201 tcagggccac caagctgcga gataatgacc ttagccaaag caggattagg gttagcgatc
    13261 ttaacggggt attgcagctt tttttcatat tgccacatta gtcaacactc ctttcccacg
    13321 gaaaaggtga tgttacccat tcccatttcc tggacgaatt gttctgcgtg atcgtcaatg
    13381 gtccgtattt ctcattgtat tgagcaataa gcttctcttt ttctgccttg tggcgtgcaa
    13441 aatatgccag accatctctg caggtagggt ggctgtcgag gtaaagatta gcctctacaa
    13501 gggcaaagct gcacgcctgt atctttctaa gaagcctctc cttttcactt agtatgggca
    13561 ttctttacag cctcctctcc tataaaaggt ttgtctatgg acgggaagat agttcctctt
    13621 gaaagtccta catctgcctc ataaggcttc tcccacatct gaaaaggcac atatgccatt
    13681 gccagcggcg tgtcctcagg aaatcttcct ttcggaaaag ggcaggcagg tgtgttatcc
    13741 tctgtgtcgt cagcaagtat cctgcctaac gacagattgt caaacatatc caaataagtt
    13801 cactccttat gttgtctgaa tctcatatac cacaggtttc gtgtggctgt tttatcatat
    13861 gcagcttata agtcagtggt gtatgtatgc aggatattat aaaaaaactt gcaaatcgtt
    13921 catttaaaat acacaaaatt aattttctgt gaacgcacat attgtttgta acaataaaca
    13981 tagtttaaat aacgttaaat ttgtaagatt taatattgac aaatcgggct tctgcgtgtt
    14041 atattaatac tattgaaaaa agcatatgca gtgacatatg tgaacgaagg agagattatt
    14101 atgaaaaatg tcaagagaat tcttgcatta acagtttcgg ctgcccttgc agtttcttgc
    14161 tttgcaggtt gcggaaacgg tgcaaaaaag aacagcgata ctatcgttat cggctgcagc
    14221 ggtccgctca caggtgacgc tgcacagtac ggcgttgctg ttaagaatgc tattgagctt
    14281 gctgtcaagg acgtaaatga cgcaggcgga ataaacggaa caaagctctc atacgttttc
    14341 gaggacgatg aggctgactc aggcgataag gctgtaaatg cttataacgc actgaaagac
    14401 aatggtatgc aggtaatggt cggaacagta acaacaggtt cttgcctcgc agttattgaa
    14461 aagacaaagc aggataacat cttccagctc acaccttctg cttcagctca ggacgttatc
    14521 aagaacgata actgtttcca ggtttgcttt actgacccta accaaggtaa aggttctgcc
    14581 gactatatcg cagaaaacaa gcttgctaca aagatagctg taatttacga cagctctgac
    14641 gcttattcat caggtatctt caacacattc aaatccgagg ctgaggctaa gggtcttgat
    14701 atcgttgcag agcagtcatt cacaaaggac agcaagactg acttctcagc tcagataagc
    14761 gcagctaaga atgcaggcgc agacctcgtg ttcctgccta tctaccatca gcaggcttca
    14821 cttatcctta cacagtgcaa gtcagctaac tacaacccaa cattcttcgg ttgtgacggt
    14881 cttgacggac ttctttctat taagaacttt gatacttcac tttgcgaggg cgtaatgctc
    14941 attacaccat ttgctgccga cgctcaggat caggcaactc aggacttcgt taagaagtat
    15001 aaggacgctt acaacggcga aacacctaac cagttcgctg cagacgctta tgacggcgta
    15061 atggtacttg cagaactcat gaagaaggaa ggcatcacag ctgatatgtc tgcttctgat
    15121 atctgcgata agctcaaaaa ggctatctct gataaggact tctcatatga cggacttaca
    15181 ggtacaggca tgaagtgggg cgaagacggt gccgtatcta aggaccctaa ggctgttatc
    15241 atcaagaacg gcgcatattc tgcactgaca gctgatgaca ttgccgcttc tactgctcag
    15301 taatagctga aatgaagtgc gtacaaaaag tacgcttaat atgaaaaaaa ccggcaattg
    15361 cgaaaaattg tcggtttttt tgatttattt cattaaaatg tgttgcaaaa nnnnnnnnnn
    15421 tatattgtgt ttatgacaag gacagatgta atatatgtga ttacatcttg tgttttgtct
    15481 attccaaaag gaaggaatgt ttgtatatgg gttttcttaa taacctcatc aacggaataa
    15541 gcctcggcag tatatacgca gttatagctt tgggctatac gcttgtatac ggtattgcca
    15601 aaatgcttaa cttcgcacac ggcgacgtta ttatggtggg cggatatgtt atattcacct
    15661 gcatgaccac atggggactg aactcttggc tatctatagt tcttgcagtt attgcctgca
    15721 cacttctcgg tatagtcatc gaaaaggtgg cgtataagcc gctcagacaa gccacttcgc
    15781 ttgcggtgct tatcactgcc ataggcgtca gctacttttt gcagaacatc gcactgctta
    15841 tattcggtga aaagcctgta aactttacat cagttgtaaa cgtgccgagc atcaagcttt
    15901 ttgacggaca gatagttatt tccggagaaa catcagttgc aataatcgta tctatactca
    15961 ttgtagttgg tctttcattg ttcatcaaca aaaccaagag cggacgtgca atgcttgcag
    16021 tatctgagga cagaggtgca gctcagctca tgggcgtaaa catcaaccgc actatctctc
    16081 ttacattcgc tatcggctca ggtcttgccg caattgcagg cgcactgctt tgctcagctt
    16141 atcctacgct tcagaacacc acaggcgcaa tgcctggtat caaagcgttc gttgcggcgg
    16201 tatttggcgg tatcggctca ataccgggcg ctatgatcgg cggtatcctt atcggtgtta
    16261 tcgagatact tggacgtgcc tatatctcac cacagcttgc cgacgctatc gtttttgctg
    16321 ttcttatact tgtgcttatc attaagccta caggtatcct cggcaagaag ataagcgaaa
    16381 aggtataaaa gtcaacttaa aattttcgag cagacttgct ttgcagttct gttttatctg
    16441 aaaggattgg ttttttgtga aaaaagaagg ggctagcgca aaaacaaagt tttttaagcc
    16501 gcagaccaga aaaagcttta tcacatacgc aatggtgata gttgcctttg tggtaatgca
    16561 gatactcagc agcggcggat atatctcaag tatgctcaca ggacttcttg ttcctctgtg
    16621 tgtatactct atacttgcag tatcccttaa cctgacagtt ggtatacttg gcgagcttag
    16681 cttgggtcag gcaggcttta tgtgcgttgg cgcatataca agtgcgatat tctctatcgt
    16741 tacttctgaa actattgaaa atgtgtggat cagatttcct ttggctatac ttataggcgg
    16801 aataattgcc gcagtgttcg gcatacttat aggaattcct gttctcagac ttaaaggtga
    16861 ctatctcgct atcgttacac ttgcgttcgg tgagatcata aagaacgttt tcgcagcact
    16921 ttaccttggt tatgattcta agggtgttca cgtttctctc aaaagtctgt caaaccttaa
    16981 ccttgagcct gacggaaagc ttatactgaa cggccctcag ggtatcagcg gcacacctaa
    17041 cgattctaca ttcattatcg gcgctgttat gttgcttatc actcttatca tcgcctataa
    17101 ctttgtatat tcaagaacag gaagagcagt aatggctatc agagataatg ttatcgcagc
    17161 cgagtctgta ggtcttaaca tcacaaagta taagcttatt acttttgcat tgactgcatt
    17221 cctggcaggc tgtgcaggtg cgctttattc tcataacttc tatacgcttc aggcttctaa
    17281 gtttgattat aacctgtcta tccttatctt ggtattcgtt gttctgggcg gtataggcag
    17341 tatcagagga agtgttatcg cagctatcgt gcttgttctg cttccagaaa tgctaagaag
    17401 cataaacagc tacagaatgc ttatctattc tatcgtcctt atcgtaatga tgataataac
    17461 ttcaaatgaa aaaacaaaga cttttatcag tgcaaatact gctaagttca aggctttgtt
    17521 caagcgtgaa aaagtaaagg aggctgagtg atatggcact tcttgaagta aaaaatctcg
    17581 gcatctcatt cggcggactc cgtgcagttg acgagttcag cataacaata gaaaaaggtc
    17641 agctctatgg tcttattggg ccgaacggag caggaaagac cactatcttc aatatgctca
    17701 caggcgttta taagccaaca gacggcacaa tcgttcttga cggaaagaac attaccggca
    17761 aaaacactat cgaaataaac cgtgcaggca ttgcccgtac attccagaac atcagacttt
    17821 ttaaggatat gacggtgctt gacaacgtaa aggtcgcact tcacaatcat tataaataca
    17881 gcacctttac aggcgttttc agacttccaa gatatttcaa gcttgaaaag gcaatggacg
    17941 caaaggcgct ggagcttctg aaggttttcg agcttgacga taaggcgagt ttgcttgctt
    18001 ctggacttcc gtatggcgat cagagaaagc ttgagatcgc aagagccctt gcaacacagc
    18061 cgaagttgct gcttcttgac gagcctgctg ccggcatgaa tccaaacgaa acagcggagc
    18121 ttatggatac tatccgcttt gtaagagata attttgatat gacggtgctg cttatcgagc
    18181 atgatatgag ccttgtttca ggtatctgcg aaaagctgtc agtgctgaac ttcggtcagc
    18241 tcctctgcga gggcaagaca gcggaagtgc ttaaaaatcc tgaagttatc accgcatatc
    18301 tgggcgaata agggggtaag taaaatggca atgttggaaa tcaatgatct gcacgtcaat
    18361 tatggcatga tagccgcttt gaaaggtatc tcctttgagg taaacgaggg cgaggtcatt
    18421 tcccttatcg gcgcaaacgg agcaggaaag accaccactc tgcacaccct tacaggtctt
    18481 atctctgcaa aatcgggttc tatcaaattc aacggcgtgg agcttactaa aactcctgcc
    18541 cacaagatcg tggaaatggg tatagcacac gttcctgagg gaagaagaat attccaaaac
    18601 ctcacagtgc ttgataacct taagcttgga gcatttacaa gaaaagacaa ggagaatata
    18661 ccgaaggata ttcaggatat atacgagcgt ttccctagac ttgcagaaag aaaaaatcag
    18721 atagcaggta ctctttcagg cggtgagcag cagatgctgg caatgggcag ggcgctcatg
    18781 tcaagaccta agatagttgt aatggacgag ccgtcaatgg gtctgtcacc tatccttgtg
    18841 tcaaccatat ttaagataat cgaggatatc agaaagagcg gaacaactgt attgcttgtt
    18901 gagcagaacg caaagaaagc ccttgctatc tcagacagag cttatgttct tgaaacgggc
    18961 aagatagttc tgtcaggcaa ggcgtctgat cttatcaatg atgagtcggt caagaaagca
    19021 tatttaggtg aataggagtt gagctttatg gcaaggatca taaccattgc aaggggcatc
    19081 ggcagtggcg gaagaactgt aggaaaaatg ctgtcagagg atatgggtat aaaatattat
    19141 gataaggagc ttataaagct tgcaagcgac gaaagcggca taaatgaacg ctttttcggg
    19201 cttgttgatg aaaaactgaa aggttctttt ctgagaaagg gcggagttta caagggtgag
    19261 cttcttgacc ctgatgatca cgactttact tctgacagaa atcttttcaa ttatcaggcg
    19321 aagataataa agcagcttgc agacagagag cctgcggtaa tagttggcag atgtgctgac
    19381 tatatcctcc gtgacagaaa agacgtgctt aaggttttca tatattctga tatggaaact
    19441 gcggtcaaga atatcgtcga tctgtatggt gtttctgtaa aagaggctgt caagataatc
    19501 gaaaagacag acaaggagag aagcgattat tatcgtcact acacaggcag ggattggaca
    19561 aatgcgaaaa actacaatct gtgtcttgat accagcgaca tgagctatga caagtgcgtt
    19621 gatatcattg agggatatat aaagcttctt gaagagtaag gcataaaaat aaagccgcat
    19681 tgcttttttt gcagtgcggc ttttgtgata tatgaaatca gttaaatttt tctgaaatag
    19741 tccttgcggc aaatactcct gatgcagaag cctgcgacag agagtgcgta acgcctgagc
    19801 cgtcgccaag gacgtaaaga cctttgacag cagtttcaag gttctcgtca acgtcaactc
    19861 ttgaattgta gaacttgacc tcaacgccgt aaagaagcgt gtcgatattc gccataccgg
    19921 gagctatctt atccagagcg tatatcattt cgataatatc gtcaagctgt cgctttggga
    19981 taacaaggct caggtcgccc ggagtagctt tgagagtagg gttaaggaac gtctgaccaa
    20041 agcggtgttc atttgtacgt ctgcctgcaa caaggtcacc gaaacgctga acaagcactc
    20101 caccgccaag catattagac agccttgcga tagactcacc gtatgcgttt gagtccttga
    20161 aaggctcaga gaatgtgttg gataccagca gagcgaagtt tgtgttctca gttctcagtg
    20221 cagggtcagc atagctgtgt ccgtttactg tgatgatgcc gtttgtattc tcggtaacca
    20281 cctcaccgta tgggttcatg cagaatgttc ttacaaggtc gccgtatttc tttgtgcggt
    20341 aaactatctt gctttcgtat accttgcttg tgatatgctc aaaaacagcc gcaggtatct
    20401 caacacgaac gccaatatca actctgtttg aacgcctttt tataccaaag ttgtcgcata
    20461 tctcagaaat ccattttgaa ccgctcctgc ctgccgcaac aacagcgttt gagcagtgat
    20521 actcgtcctt gtctgtgata acagtgaagt cgttctcctt gcctgttata ccctgaacac
    20581 tttcataaaa ttttatgtct atcttgtctt tgacaaagtc atagatgttc tggagtatct
    20641 gcatatttct gtcagtgcca aggtgcctta cctgagcgtc aaggaggtgg aggtcatatt
    20701 tcagagccat tgccttgatg tcggaagatg ttgtggagta aagcttagcg tcctgaccgc
    20761 ccatttcaag gtttatcttg tcaacatact gcataagctc gatagccttt tccttgccgg
    20821 taaatgtgta aaggtcaccg ccgaaattgt ttgtgatgtt atatttgccg tccgacattc
    20881 cgcctgcacc gccgaagccg tgcataatgg agcatacttt gcagtgaatg caggacttaa
    20941 tttttttgcc atctatagga cacttacgct tgctgacagg tccgccctga tcaataatgc
    21001 aaacttttat gtttttgtca agcttgacaa attcgtatgc catgaatacg ccggctgcac
    21061 ctgcaccgac aacaactacg tcataattct gagccataac tatttccttt caaatcaaaa
    21121 tccaatttat ttttatccat aaaacgggcg gccgcacctt tgcagaagat agcctgaaag
    21181 gagataaccg ccatgttttt tttattttat atcaaatacg atagtagcca atgtgaagaa
    21241 tatcagtgtt gagcaagtgt caacgatagt ggtgataagc ggggcagcca tgatagcagg
    21301 gtcaagatga agcttcttgg cagccattgg gagcatacag gcgatgagct ttgaaagcac
    21361 aacagttgcc atgatagcca tacttaccgt gaatgcaagc ttgtagcaat ccacagatgt
    21421 gtcaccgtga tacaccagaa ttattcttat agcattgact atagcaagga taatgctgca
    21481 aagaagtgcg actctgaact ctttccacat gaccttgaaa aagtctttca ttcttatctc
    21541 accaagcgcc atgcctctta taacaagggt cgaggtctga gagccgcagt ttccacctgt
    21601 gccactaagc attgacagga acgataccaa aacagggaca gccgcaaaag cagactgata
    21661 tttgtttacg atagcacccg taagggtagc cgacagcata agtatcaaaa gccatgctat
    21721 tctgtttttc gcatgagtga aaacagaggt cttgaaatat gaatcgtcac ttggcgcaac
    21781 ggcagccatt ttcgagaagt cctcagtggt ctcctcctcg ataacgtcca tagcgtcgtc
    21841 aaatgtaacg ataccgacca tgcggttttc tctgtcaaca acaggcagag aaagaaagtc
    21901 atattttgag aacatggaag caacatactc tttatcctca agggtatcaa cggaaataac
    21961 gttgtcctcc attatgtcct ctattttatc gttatcatca gcagttacga tatcaagcag
    22021 tgaaagcacg cccacgagct ttctgttctc agtgacataa agtgtattta cagtttcctt
    22081 gaccatacca acgtgtctta tccaatccag tgcttccttg gcggtgtagc ttctgtgaag
    22141 ataaacgtac tcggtggtca taatagagcc tgcactgtct ttaggatatt tgagtatctg
    22201 atttatctgc tttctcgttt cagcgtcagc agacttgact atccttgcaa caacatttgc
    22261 aggcatttcc tcgataatat ctaccgtatc atcaagataa aggtcgtcaa cgatctcctg
    22321 aagctcctta tccgtaaagg cattgataag catcatttgt gtatcgctgt ccatataagc
    22381 gaaaacgtca gccgcaacgt ccttgttcaa aagacggaac agcagcggaa gctcgtattt
    22441 ttcgacctcg tgatcgtcat aaaactgttg caaaagagca gccacgtcgg cttcgttcat
    22501 atccataaat atttcacgga tagccttgta gtttcttttc tttaacagct ccaatactgt
    22561 gttaaacata atgtttcccc ctaacagcag cggtaataag cacaacagta caaataattg
    22621 tacttatacc aactctgtac aaatataagg atctaccaaa acacagatcg tcctattcaa
    22681 ttaaaattga tatttagatc acaaaaaggc tgccacaaac gggcgagccg ctctgcaatc
    22741 gaggggcgat cgtattctga ctggcgtcgt ccatttgtac acactcctta aaaatcatga
    22801 tttatgcacg tttttactat tcgcacacat aatattatta tacgcttacc ttgttttttt
    22861 gtcaagaaat tgtttaattt aattttatga acgtgctgta aatttgcgan nnnnnnnnnc
    22921 aggtccgaat aatacgtcaa aatcatctcc gtaagtataa tgaattgctt ttgacctctt
    22981 gccaaatggg gagaaatagg gtataattat tatatggatc gaataataaa ccgaaaggaa
    23041 ttgataaaat gagcgaaata agagatgaaa gcttgtggga aagcggtaaa agaaagatag
    23101 aatgggtaag acagaatatg tcattgctca gaagcgtcga ggaagatttc aagagagatc
    23161 agccttttaa ggggctgaaa gttgcacttt ctatccacct tgaagctaag accgcttatc
    23221 tttgcaaagt ccttgcagca ggcggtgctg aaatgtacat cacaggaagc aatccgctgt
    23281 ctactcagga cgatgttgcg gcaggtcttg tgcatgacgg cttgaacgtt tttgcatggc
    23341 atggtgcaac tgacgaggag tatcacagac atatatccga ggtagttaag gtaggtccaa
    23401 acattattat agatgacggc ggcgaccttg taaaccttat ccacaatgaa taccctgagc
    23461 tgataccgaa cgttatcggt ggttgtgagg aaactactac gggcattatc cgtcttattg
    23521 caatgaacaa ggacggtcag ctcaaattcc ctatgatgct tgtaaacaat gcaaagtgca
    23581 agtatctttt tgataacaga tacggcacag gtcagtctgt atgggacgga ataaacagaa
    23641 caactaacct tatcgttgca ggcaagaacg ttgtcgttgc aggctacggc tggtgcggca
    23701 agggtgttgc aatgagagca aagggtcttg gtgcaagcgt tatcgtctgc gaagtcgacc
    23761 ctataaaggc tatggaggct gttatggacg gcttcaaggt aatgaaaatg gttgacgccg
    23821 ctaaggtggg cgacttcttt gttactgtaa caggctgtaa ggacgttatt actgagaaag
    23881 ctttcatgaa catgaaagat ggtgctatac tctgtaacgc aggtcacttt gattgcgagg
    23941 tagacgtggc tggacttaaa aagctgtctg ttgaaagcaa gcttgcaaga aacaatattg
    24001 acggttataa gcttccaaac ggtaattggt tgtatgttat cggcgagggc agacttgtaa
    24061 accttgccgc aggcgacggt caccctgccg agataatgga tatgtccttt gctatccaag
    24121 ccctttctgc ggcttatctt gttaaaaaca aagacaagct tacagacaag atagtttatg
    24181 ttcctgatga gatagacaag gaagttgcaa acagaaagct taaattctgg gagcttgaga
    24241 tagataagct tacacctgag caggagaaat atcttaacag ctggcaggta taaaatctca
    24301 tattagtaca ataaaacgga cagctttggt tgtccgtttt tttgtatctt gaaattgcca
    24361 taaatttatt ttgtcttcaa ggttttaaat cactgatttc tgtacatttt aatattaggg
    24421 cgataaaaaa acagggcggt gagaatgtgg cggcaaagat gtataagata cttacgcttt
    24481 tttcagtggg tgcaatatgc tatggcttca tggaaatact taacagggga tattctcata
    24541 tcacaatggg ggtgcttggg ggaacttcaa tgctggttat ccatatcatg aatggcgagc
    24601 gcaggcgagg agtgagcctg ctgtctgtgc ttgcagtgtc ggcagcattt atcactgcca
    24661 tagaatttct tgcaggggag tatctcaacc gctatctcgg catgggcata tggagctatg
    24721 aggaagtgcc gctgaatttt gacggacaga tatgcctgcc atttgctttt gtgtggtttg
    24781 ggttatccta tattggtgtg cttgcggata attttatacg cagacatatt ttcaaggaat
    24841 accctgtcat tgtcagccgt aagaaagaga aaatacctgc cacggttgga taaactaata
    24901 attagcgtgc gaccttaggt cgcccctacg cagaaattgt cttattaacg ttaaatgtag
    24961 gggcgaacag tgttcgcccg atgtatacca acgcttatat ataaacaaac ggactgtcta
    25021 tccaatgata aacagtccgt atttttgtac atatcaatct tttgtgtacc actttttcag
    25081 cacttcttcg ccaagcttgc cgtaaactgt gaagtcacgg tttttcttgg cttcctctat
    25141 tggctgcagg atagctttcc aatcccacca gaaatagccg cagaaccaat ccagatccca
    25201 agttgcttcc attgcagtgt cgtagaaatc tgcctgctcc tgctcgtcaa gtggcttgtc
    25261 gagcctgtca tggaaatccc aaggatactg cgtgcagccc tcctcgtttc gcacgcctat
    25321 ctcagcgaac attataggct tgttgaaatg tttgtgagcc ttttcaaggg agccgacaac
    25381 ttcgttccag cgactgagca tatgctcctt gcttctgctc acaccgtctg taacagggta
    25441 gtagcctgag atgccaatga tatccacgtc gctgagccaa tcgaaacgct gttcgtcgcc
    25501 gtgattgatg ttgtgcataa cgataccgct gtagacctct ctcacctgtg cgataagctt
    25561 tctgcatctg tcggactgct tgtccattcc tgccatttca cagcctgtgc agagcatttc
    25621 gcaaccgagc ttttctgcaa gctttgcata gtgaagcatg aaagctgtgt atgacttgaa
    25681 ccacttgtcc caatattcag ggttctcagg cgggaagtct atccttgcac gccatgaatg
    25741 gtcaaggcaa tccaccatag gcttaaggca gactttaagt cccagtgact ttgctttttt
    25801 tatagcgaag gcaatgtcct cgtcagattg tgttctctca taatcactga aaactcttgt
    25861 gctcgcaaag ctctcctgca atgcgtttac agggatacat atccagttca agccgtttga
    25921 agcaagcctt tccattgact tttctgccgc ttccgtctga aaatcacctc tcttggagaa
    25981 aaatccccaa gtatatcctt tgcagaaaat atctttattc atgtatcaaa aacctctctt
    26041 ttttgtaaat gtcctgatag aaaagccaca ggtaagctca agcctgttca cctttttaag
    26101 cttctccacc gcctcggcag atgttttgta gtaatatgga gtcattgtaa aaagattgaa
    26161 aatatcctcc tgactttcaa gcaccacagg atattcaaaa atcatctcct ctgactcgtc
    26221 aaatttatta agtccgtata cattgggctt gttttcgtaa ggcttctcat aaagcacttc
    26281 tttaagctca aatagatgtc ttggggatgg agaaactacc acaagctttc cgccgtcttt
    26341 cagtaccctt gcgtaatcgt catttgaaac aggtgcaaat gtgcatacga taaggtctgc
    26401 aaatttgtct ataaacggaa gctgaaatga cgatgccact gcaaattcgc aatttgtgat
    26461 attttcgcaa tgtatcctcg tcatgcagtg ggcaactgcc gcctttgata tgtcaatgcc
    26521 gtaaaacttc gcctttggca gtgccttggc atagttcaca gtgtaaaaac cctcaccgca
    26581 gccgctgtcg ataatataag gctgtttcac attctcaagc agaccgccca ttacctcagc
    26641 tgttttcttc gcaagcggca gataatagtc acggtcaagg aaatccgttc tcgcctttac
    26701 catttccgca ttatcccctg cgttcttagg gtttctgccc tttgttgtaa gcagatttac
    26761 atatcctttt ctcgcaatat caaagctgtg accgttctga cagtgccata cgttgtcaag
    26821 ctttctgagc cgttttttgc atatcggaca aacatacata taaatcaccc tgaacattat
    26881 agcatttccc tctgctttta gcaatggata ttttattaat aaaaaagtgc ttgacaatcg
    26941 tcgggttatg ctttataata tcaatatatg caaaatgcgg tgacggaatt atccgaaaca
    27001 gtgtcggtaa agagaaaagg cggtgggtga aagcctttgc ggcaagttcg gtggctaacc
    27061 tgagcggtgc tgaaaacagc aacgtatgct ctgcgttaaa gggcttcgag tggtcgggcg
    27121 atgagttcgg caatttgggt ggtaacacgg aactgtcggt ttcgtcccat gtttagggac
    27181 gagccgtttt tttattttgc gaataatttc atttggaagg atctgaattt atggaatgga
    27241 caggacttaa cgaactgcgt gaatcttatc tgaaattttt tgagagcaag ggctgcttga
    27301 gacacaagag ctaccctctc gtacctcaga acgataacag cctgctgctt atcgttgcag
    27361 gtatggcacc tttgaagcct tacttcacag gtcaggaaac acctccgaga acaagaatga
    27421 caacttgtca gaagtgtatc agaacaggcg atatcgaaaa cgttggtaag acggcaagac
    27481 atggcacata ttttgaaatg cttggtaact tctcattcgg cgattacttc aagaaggaag
    27541 ccattgcatg ggcttgggag tatgttacac aggtgcttaa actgccaaag gacagacttt
    27601 atatctctgt ttatgaggac gatgacgagg ctgaaaagat atggcatgag gacgctggcg
    27661 ttccaatgga tcatatcgta agaatgggca aggaagacaa cttctgggag gctggaacag
    27721 acggcccttg cggtccatgc tctgagatat atttcgacag aggagagaaa tatggctgtg
    27781 gcaagcctga ttgtaaggtc ggctgcgact gcgacagata catggagttc tggaaccttg
    27841 tgttcacaca gtttgaaaga catgaggacg gcacttatac tccacttaag cagaaaaata
    27901 tcgacacagg tatgggtctt gaaagacttg cttctatcat gcaggacgtt gactctatct
    27961 ttgacgttga tacagtaaag gctatcagag atcatgtttg taagatagca ggctgtgagt
    28021 acggcaagga gtacaaaaag gacgtttcta tcagagttat cacagaccat atccgttctg
    28081 taacattcat ggcgtcagac ggaattctgc cgtcaaatga gggcagagga tacgttatga
    28141 gacgtttgct cagaagagca gttcgtcacg gaaagcttct cggcataaac ggacttttcc
    28201 tcaaagacct tgtaaaaact gttgttgact gcaacaagtg tgagtataac gagcttgaag
    28261 aaaagtatga ctatatcgta aaggtgctca caactgagga gcagaacttt aatgccacta
    28321 tcgacagagg tatgaagata cttgacgatc ttatcgcaca gatgcagaaa gacggcaaga
    28381 cggagcttga cggtgagagt tgctttaagc tttccgacac atacggtttc cctatcgacc
    28441 ttacaagaga gatccttgaa gaaaagggca tgacctgtga tgaggagggc tttgctgagt
    28501 gctatgcaaa gcagagagaa acagccaaga acgctcatgc tgcaacaaag tatatgggtg
    28561 cagacgaaac tgttttccat aaaatagaca aggatttcca cacagagttc atcggctatg
    28621 acaagctcga gggcgacagc gagatatcat tcattacaac tgatgatgag cttatcgaaa
    28681 acgcaaaggc tggcgatgaa gtatatatcg tatcttcaca gacgccattc tatgcagaga
    28741 gcggcggaca ggtgggcgat atcggtacta tcgttacaga aagcggtaag tgcagagtcg
    28801 ttgacacaca gaaggtcgtt gcaggcaagt tcgctcataa ggcagttgtt gaagagggcg
    28861 agatagctgt aggtcagaag gctcacttca ctgttgacag aaagacacgt tatgctgtta
    28921 tgagaaatca tagctgcgca catctgcttc aggctgcact gagaaaggtt ctcggtgagc
    28981 acgttcatca ggcaggtcag ctcgttgacg ctcacagact tagatttgac ttctctcact
    29041 tcaacgcaat gacagcagag gagaagctca aggtcgaagc acttgtaaac aagtatatcc
    29101 ttgaagctct tgatataaaa atggaagaaa tgcctatcgc agaagctcag aagcttggcg
    29161 ctatggctct gttcggtgag aaatacggtg aaactgtaag agttgtaaca atgggtgacg
    29221 gtgacgaaac agcttctatc gagttctgtg gcggtacaca tcttgacaat acttcaagaa
    29281 taggtctgtt caagataatc tccgaaagct ctgtagcttc aggcgtaaga cgtatcgaag
    29341 ccgtaacagg cagaggcgtt ctggagcttg ttgaggaaag agccgcaact atccagcagg
    29401 cggctgaaag ccttaagctt gcaaatccac tggacatcgt aaagagatgt catgctatca
    29461 tgcaggaagt caaggatctt gaaaaggaaa gagacgcttt gcaggctgag ataacaaatc
    29521 tcaaaacaaa gtcactgttc gataatccgt atgaggtcaa cggcgttaag gtggtcactg
    29581 caatgctcac aaatacaaga cctgatatgc tcagaaaaat gggcgacgag cttaaagcaa
    29641 gagaagctga cgcagttgca gttgttgcag gcgttgacgg tgaaaaggca aaccttgttg
    29701 tagtttgcgg caagaatgct gttgcaaagg gtgcacacgc aggcaagata gcaggcaagg
    29761 tagccgcact cacaggcggc aagggcggcg gacgtcctga ctctgctatg gctggtatcg
    29821 gcgacagatt caagatcgac gaagctctcg ataaggtaaa cgaaatagtt ggcgaatttg
    29881 taaaataata tgggcaaagg ctgtgcaaaa tgctgtgcag cctacccctt tttgaaaggc
    29941 ggagagataa atggaagatt gtcttttttg taaaattatt tccggggaaa taccttcaaa
    30001 gaaaatatac gaggacgagc tgacatacgt ttttgaggac attgctccta cagctccgat
    30061 tcattatctt gttatcccta agcagcatat ttcaaagctt gacgagataa cccctgagaa
    30121 cagtgcagtt atcgcgcata tctatgaagt tatcgcaaag cttgccaaag acctcgacct
    30181 taaggacggt ttcagagttg tttccaactg cggcgagcag gcaggacaga gtgttttcca
    30241 cattcatttt catcttcttg caggcagacc acttgcttgg ccagccggtt aaagaaataa
    30301 aacgggcggg taaatttctt atccgccctt agttttaagg gggatggcgg caatggtgag
    30361 aaaaagctgt tatatcggct tttcgtattt tgcagggctg tttctctgta ccattggctg
    30421 gggaaggaac aacgcagttt ttgcacttgc gtcagcgtgt gtagccatta cagcctttgc
    30481 gctttttaag agataccgaa agcatatagc tctctgcttt gtttcgttcc tgatagcggt
    30541 gggagtcaat accgcttata ctcacatttt ctatgataaa ctgacagctt tagacggaaa
    30601 gaccgtaaca gttaaaggcg agataaccga cataagctat ataggctctg acacctgtcg
    30661 actaagtgtc aatggtacca ttggcgaagt aaaaggcagg ctgacctttt atgcagatga
    30721 cattgaatat gattactatg acaatatcgt ggcgacagta aaggtcagta ggatagaaga
    30781 cagtatcaat ttcaggtctg agcagtataa cagacctaaa ggcgtttttc tcaaaggctc
    30841 gaccgctgaa agcatagaag tcacaggcgg cggaaacacc attttacgca gtataatgca
    30901 ctatcgtgac aagatgttca tgttgataaa cgatatcata ggcggagatg agggcggttt
    30961 tgccgctgca atgctctgcg gagataaaac tgagctttca aagcaaacga agctcacggt
    31021 ctatcgtagt ggcataggtc atatattttc cgtgtcggga actcatgtgg tcataataag
    31081 tgccatgata ggctggctca tgcagaaact aagcaaggat aagcgtgtga tatttgctgt
    31141 ccaaactgtg gccatatggg cgtttgcggt ctttgcagga ttttcagtat ccgttgtgag
    31201 ggcggctgtt atgctgacct tggtcactgc cgcaccgctc ctttaccgca gaccagaccc
    31261 agcaaatact ctctgccttt gcgctgttgt aatactcact ctttcaccct atgcggcggc
    31321 agacagttcg tttttgctgt cattcacagc tgcgtttgtt ataagcgttg tatgcccgaa
    31381 agcggtgacg atggtaaagc gcagggatat tcttggcaac attgagcgtg aaatagtcac
    31441 agcggtgacg atacttcttt gcacaatgcc tgttcagcta atgtttttca gcgaaatatc
    31501 tcttgttgca ccactatcaa atatcctgct ggtgcctgtc tgtacccttg ctctgggtct
    31561 gacggtaata actgcggtaa caggcggtgc ggcaataata gcctatcctg tgctgacggt
    31621 ggtaaagtat cttctgaaag ttgttctgtt tcttgctgag ctgttttcaa aattcagctt
    31681 tgcctatctt cctcgtggag taagaccact tggtattgta atgctcgtta cctgccttgt
    31741 acctgtggcg gtcatgtttc tgagaaagtc agtaaagctt ggggcttttt gcactgctct
    31801 aatgcttgta tgttggacag cggtgtataa tttttctctt gcttttggag gctcggtaaa
    31861 gatagttgta atgccaaaag gaaatgcagt gcaggctgtg ctgtatgata aaacaaaagg
    31921 tatggttttc gatatgggca gtaagggtaa gctcaacagc ggtatggaaa gctttttgga
    31981 gctttacggc ataaaagagt taaaaggcgc atttatagaa agcgaacagt attacactat
    32041 aacgaaatat aacgagcaga tgactataca acctgacaga ttttatataa atggcgagcc
    32101 tgataatgac agcgaactgc cgttcatgac aggcgcacag cttgattgga acggtgttaa
    32161 gatagaagca ctggaggacg gctataaaat taccaccgag ggctgtgtgg tcactataga
    32221 aaaaggttct gtcaccataa acggcagaaa ccttgacaca acagatgaag aatacccatt
    32281 gatgatagat atgaaaaatt cgcagataag gaggacggac tatggctttg cttacggctt
    32341 cggctcttgg taaaacgata agagagaatg atatcgacag cctttattat ttttacggac
    32401 atgatacagc ggcacttgaa agctttacaa aaaggcttgt gaacaagctt tgccctgctg
    32461 acgcccaggt catgaacttt cataagcttg acggcaaaaa tcttgatttt ccaatgctta
    32521 cggacgcttg cgaagctctg ccttttatgg ctgaacgtgt ggtggtgact ataaacgacc
    32581 ttaatataga cgcagtgact aaggacgatt tggacgacct gaaaaagatc ctgggctctc
    32641 ttggcgaaac caccacagtg ataatctatg ccacaggagt tgaccttttc aagaacaaaa
    32701 agtatcttac agacaaaaac aagcgctttg cagatttctg cgaaaagcat ggcactgtct
    32761 gcaactttga atacaaaagg gcttccgacc ttggaaagtc tatatctacc tatcttgcaa
    32821 aaagcggctg taccataaca aaaagcaacg cagagtatct tgcaaacctc tgtttgtgcg
    32881 acactgcatt tatcagcaag gagcttgaaa agctttcggc ttatgcagag ggcagggagg
    32941 taacaagaga agatatcgac ttgctttgta tcaggcgtat cgagtcggac ggatattctc
    33001 ttgcaataaa tatccttaga ggcaatgcgg caatggtctt cacacgtttg cgtgaactcg
    33061 atgtgcagaa ttatgaacca tatgctataa taggaataat cggcttttcc cttgcggata
    33121 tctatagggc gaagcttgca cgttcctctg gcaggagcgt tgcagatgtg gctaaagatt
    33181 tttcctatgc aaaaaacaga gagtttgcag taaaaaacgc ctattccgag tgcggaaata
    33241 tctcggcgga gcgtataaga aaaacgcttg agatactttc tgaaaccgac cttatgctca
    33301 aaacaaggtc acggggaaaa gacggagata tgctcaccct cgagcagggg cttgcaaggt
    33361 caatggcgtt aaggtgttag taaatggaaa aacttaaaat atcaagggca ataatcgttg
    33421 agggaaaata tgataagata aagctttcgt ccatagttga cggcgttata attgtcacta
    33481 acggctttcg tatctataaa gaccctgaca aggcagcgct tataagatac tatgctcagc
    33541 atggcggaat agcaattctt acagactctg acacagcagg ctttcgtata agagggcata
    33601 taaagggtat cgtccccaaa ggcgatatcg tgaacgtgta tgttcctgag atattcggca
    33661 aagaaaagcg caagacagag ccgtcaaaag agggcttgtt aggggtcgag ggcgttcctg
    33721 cggatattat cagggaagca tttgaaaaag caggcattat aggcgaaaag gtcgcagaca
    33781 gagaacttat gaccaagctt gacttttatg agctaggtct aagcggcaga cctgacagca
    33841 gcaggctcag acagcttgtg tgcgaaaagc tgtcgctgcc caaaaagctc actgcaaatg
    33901 cactgcttga agtggtgaac acaatgctag ataaagaaag cttgcaagca ctcatagagg
    33961 aattgacctc agaaaacaat aaagacaagg agaatagcgt tggttagtct tgaattttta
    34021 tatttctttc ttcctgtttt tatgggagta tacactttgt caggggtgcg tttcagaaaa
    34081 gccgtgttcc tgcttggaac agttgtgctg tgtctgtggg tgtccacatg ggggataata
    34141 ccaatggcgg tgtgtgctgt tgtatcatac ttttgcggca ggcttatcca cagctttgca
    34201 gacaaaaaag gacttaaaag gctatggctc atattatcgg tggtagtaaa tatggcagtg
    34261 cttcttgtat tcataagaag cggtcacagc agctatgacg tgacgtctat attcacaggc
    34321 aaaggcgcaa tgctaaagct tttcccggtg tggggagcgg gcattttcac tctccatggc
    34381 atctcctact gcatggatat ctataggaaa gaaatagagc ctgagaagaa ttttcttttg
    34441 gtatctcaat atatatgctt tttcccatgc tttagctgtg gaccgatact aagattttcg
    34501 gatatgagcg aacagcttag aaaacctgtc ataacctctg ggaagcttgc agagggcata
    34561 aagcttatgc ttataggctt tgcggagaag ctttttctgt cggatcctat gtacgagatg
    34621 tggcagcaca taaggggagt aagtacagat tcgctctccg ccgcttgtgc atggcttggg
    34681 ataatagctt tctcatttgc gttctactat gagcttagag cttattcgca tattgcacag
    34741 ggtattggtt caatgctcgg ctttaaactt cctgataact ttgatctgcc gtttatgtca
    34801 gcaggcttca atgagcttat aaaacgattt gcctgcacct tttatagttg ggtgagagat
    34861 tatttctaca gacctatatg tagaaataaa gacagcgttt gcctttgggc attgatactg
    34921 tctgtgcttg caggctcggt ttggtatggc tacggcagac acacaatgct gtgggcagcg
    34981 tttatttgtg ttatgcttgg catagaatat ctcttgaaaa acattcttgc ggctgtgccg
    35041 acaggggtaa gatgtgcggt gatgaatgtt ctgtttctta taggtctgcc gtttcttgcg
    35101 atagacagcc tgccttcagc ctgcggatat gttgccgcaa tgctcggcgg gggaagcttt
    35161 gcaggagatg ttcttgcatc gtatgtgata aaaacaggag ttgtgatgta tcttatctgc
    35221 ggcttttttg caacaggtat cggcagatat ctcaaacgca ggataatgca gctcaatact
    35281 aatatagttt acataatcga gccaatgctc actataggat ttctgcttat cagtacagcg
    35341 tttcttgtgg gcggaggtaa ttcgtctatg aatttgttcg tgagataaaa tgtgaggagt
    35401 gaacattaat gaaaaaggct gctgattata tcgcagtaag cgcatttttt gtaatagtca
    35461 taatgtttgc ggcatatact ctcgtgatgg gcagacatgg atctgaaagt gaaaaggcag
    35521 acagttcaga tataagacat aacgttcaga cctatgttag cagtaatttt cctctaagct
    35581 caaattggaa gtcgctcaga acgaccatgt ttgtgatgac tggtaagaaa aattttgata
    35641 atatctatat ccttaaaaat cgtcttgtag agatcacaga ttttgataag gacaggcttg
    35701 ataaaaatat ctcgcagata aacgattttt cagaaaacac agatacacct gtatatgtta
    35761 tgttagctcc gactgccgca ggaatctatt ctgctaagct gcctgcttat acttcaaaca
    35821 cagaccagcg tgaactgata gataatatct attatgacct agacagcagg atagacacca
    35881 tagatacgtt ttttcctatg tattctgcaa gaaataatta cgtttactat cgtacagata
    35941 gaaagtggac ttccttcggg gcgtattttg cctatgcaga cggcataaaa gagctgggtt
    36001 ttgagccggt cgttctgtcc aattacgatc aagaatatac ctcaaattct tattatgggg
    36061 gactttacag tgaactttgc tataacagca taggcgcaga cagagtgaac atcttccgtt
    36121 caaagtatga gactactgtg gattctgtgg agctttacag agaagatcag gtgcttacct
    36181 caaaatctgt ttattttaga tctgcactga aaacagcaga taaaactgct atcttcctgc
    36241 aaggtgacaa ctttactaag gcaacggtca agacaactgc agaggacgca ccaaagcttc
    36301 ttatcatcaa aggaagctat gccaataccc ttgtgccgtt tcttacccca cactacagcg
    36361 agataacctt ggtagaccct gacaagctca aagaggaggg caagaccctt tctgacgtgg
    36421 cagatacgag tgcgtatgac cagatattat ttatgtatga ctgcgaccag tttgcagatg
    36481 aaacaaattt tgacctgctt aaataatatg ggctatgtac ttgaaaaaat ggaacagatg
    36541 tgatataata aaggaaatgc cgtacaagga atgtgcggca taatgtatgt ctgaatattt
    36601 gagagggtgt ttgatgtgag tgagcaaaaa aacagacttg atcttattga tcttaatgac
    36661 tatccagtat cttggtggct gaaacttctt gacctcggtc ataagatagt tcaggatcct
    36721 gccgcctaca gcgaggcttg caagggcaag ataatgggta ctcttttcta tgagccgtca
    36781 acaagaacgc agatgtcttt ccagaccgct atgctccgtc ttgggggaac gataataggc
    36841 tttgacaatc ctgccacttc ttccgttgca aagggagaga atatcaagga taccacaaag
    36901 atagtttccg gctatgccga tatcatggta atgcgtcacc ctaccgctgg ctcggtaaag
    36961 gctgcggcac tcactgctga ttgccctgtt atcaacgcag gcgacggtgg acacctccac
    37021 ccaacacaga cacttacaga cctgcttaca cttagagaag aaaagggcag acttgataac
    37081 ctgaccatag gcttttgcgg tgaccttaaa aacggcagaa cagtccattc ccttattaaa
    37141 gcaatggtat gttttccaaa caataagttt atattagttt caacttctga gcttgctttg
    37201 cctgtgtatg taaaggatat ccttgaagcg gcaggcatgg agtacaaaga agttaccgac
    37261 cttgaagagg tcatcggtga gcttgacgtg ctatatatga caagaataca gaaagagcgt
    37321 tttgcttctg aggaggagta tcagactcag agcggaaatt acatactcga tacaagaaag
    37381 ctttcaatgg gaaagcctga ccttatcgtg cttcaccctc tgccaagagt cgatgagata
    37441 gctatcgagg tggacgacga cccgagagca atgtatttca aacaggcaaa atacggtatg
    37501 tatatcagaa tggcactcat acttacagtt ataaacggcg atggtgcttc gcagccgctt
    37561 atgaagggca gagtgcataa gcacattgat tgttcaaatc ctaactgtat cactcaccac
    37621 gaaaagtatc tgccacattg gttcaaaggc gacggcacaa cgcttgtttg cgaatattgt
    37681 gacgagagaa cattggtgga ataaaataag agcttaatgg ggtaagcaaa ggctgtgtgt
    37741 cagcctttgc ttaccctttt ttattgtgca gatagcgtat attaatagcc ttgtgatatg
    37801 caaaatcgtt aaaagtaacg aaattcttac aatatggtaa caaacagtct tgaaataatg
    37861 ttgagcgtgt ggtataatta aaatggataa attttttaca ggagctttga tctaatgaat
    37921 acactttcag atgaggagtt tgaacagatc tacggacgaa agcctgtcag aagggtgaaa
    37981 aagaaaaaaa tatactggaa cagaattatt attgcattga taatacttat cggcattgtg
    38041 actggtctgg taaagctgac agccttggta gtatcaaaga tcaaaggaga cgatacgcaa
    38101 aaaacaaaca aggtattgaa taatacatct tctgtcgctg tgaaggagga cgcttcttct
    38161 gctgaggata aaaaagacga gcctgcgaag tattcaaata tggactttac agtgtgtgtt
    38221 gacgcaggtc acggtgatta tgacggcggc acaactgacg cctcaggaac aagatatgaa
    38281 aaagacgata acttaaaggt cgcacttgag gtgcagaaat atctcgaaaa atacggcgtc
    38341 aatgtggtga tgataagaga tgatgactct ttccttgagc ttgacgaaag atgtgcaaag
    38401 gcaaataacg ccaaggctga tatgtatgtt tcacttcacc gaaacagcta tgacggtgat
    38461 atttcgggcg ttgaggtttg ggtgaataat tctgagcctg aatatgacac aaagcttgca
    38521 cagaatatac ttgatgatct tgaaaaggta ggtatatctg aaaatagagg agtacagtat
    38581 ggctacgttg gcaattccgg tgtaaactat tacataaatg ccgacactgt tatgccaagc
    38641 tgtcttgtgg agcttggatt tataaccgag gatatcgaca ataagctttt tgacgaacat
    38701 cttaccgagt atggacaagc tattgctgat ggtatagttc agacagctgt tgatgttggt
    38761 cttgttgatg aaagtggcaa aagacttatg tctgagcagc ttatctcacc tgagaagcct
    38821 gtgaacaata aggattcttc atctgcggct gacgctatga caacgccgat ggagacggat
    38881 tcttctgctg acgtttataa tactcaggaa aacgagtggt attgataaca aaaaaataaa
    38941 agcgaacaca gttcgtttct gctaatgcag agaaaccgtg ttcgcctttt tgttgctaaa
    39001 gctcttttct aatgagtttg aatttctcaa attctttcgt caggtctgcc agaacgtttt
    39061 caagaccttt ttcagagtat tcttcgtgat attctgccac aaagtctgtt acctccgtga
    39121 cgcactttgt gccgtaccat actctgacat aaagctcttt ttcatcgccg agaatagggt
    39181 ctatgcggta gctgaaattg gtgtaattgc tgccagacca ggtgtttttt tcttcaaaat
    39241 agtgaaatgt gggtatatca aaataaccat ggagcattgt atattttcct ttcaaaaatc
    39301 attcgtttat gatattatag catatcactg ttcctttttc aagcatttag tctaattctt
    39361 aattaaatta acctaaaaaa cgtttacaat tttgttagga atgtatattg gagaattgtt
    39421 caaaatatgt tacaattaat aatggtatga ctatttcagc ggtatgctgt cgcagaaagg
    39481 ataagatcta tgagcattac aataaaagat gttgccaagg aagccggtgt ttcagtggcg
    39541 acagtgtcaa gggttctcaa cggaagctgt aacgtatcag atgagtcaac aaaaaaagtc
    39601 aatgaaacta ttgcaaggct caactattct ccgaattttc tgggaagaaa tcttagaaag
    39661 tgcgaaacga atgttattct tgtaattatg ccgacctctg aacattctct ttattcaaag
    39721 atagtcatgg gtatgcagga ttatgcagga caagttggtt acgatatcat aactgcagtt
    39781 tcctattcaa cctcgaccgc tgaaacaagg cagatgaata tgctttttaa cagaactgtt
    39841 gacggtgtta tccttcttgg cactcagtat gacgcagatt cacttaataa gctcgcagag
    39901 aattataacg ttgctctgtg ttgtgagggc gtggagggcg caaatgtgct tactgtcgct
    39961 gttgacgatg aacaggctgc gtttgacgct gtcgctgctc ttatcaaaaa gggtcataaa
    40021 aggataggct ttatcggaac gaactcttcg gctatctcct ccactgcaag agagaacggc
    40081 tatataagag cattaaaaga atatggcata gcaataaaga atgaatatat ctataaagag
    40141 tcttatgact atgactgcgg aggcagagcg tttgaatatt tcaatgcact tgctgagaag
    40201 cctacggcga ttttcgctgt gtcagacttg cttgctatct ctgcaatgca caaggcaata
    40261 gatatgggca ttgatgtggg caaagatgtc gccataatgg gatttgacaa tatatccatg
    40321 tgtgagtgga taatccctac gctttcaacg gttgaacagc cttgctcaaa aatgggcgag
    40381 cttgttgtgg caaagcttat tgagaacatg aataagacta ctaaggataa tcattattat
    40441 actgttgacc acaggataat tctaagagag tccacaggcg actaattact ttcgtaaaaa
    40501 tagatagggg agaaataagg cagtttttcc gtaaaaactg cctattttct gtaagaaagg
    40561 attttttagg gcatggagaa atttacaaaa atacttgaaa cgatagatgg ctatgtctgg
    40621 ggcataccgc ttatcgtact cattatggca gtgggaatat tcctcacctt taggctaagg
    40681 cttcttcaaa tattccacct gccaaaggct ttgaaattta tgtttcagaa cgaaaatggc
    40741 ggcaaaggcg agatatcgag ctttgcagca ctttgcaccg cactatccgc aaccataggt
    40801 acgggaaata ttgtcggtgt tgcaacagcc attggtgtgc tttcaggcgg acctggagcg
    40861 ctcctctgga tgtggatagc tgctctcctt ggtatggcaa caaaattttc cgagggcttt
    40921 cttgctatca aataccgacg tatggacgct gacggacacg tttgcggcgg accattctat
    40981 tacatagaat acggcatggg taagaagtgg aagtggcttg caaaggtatt tgcgttcttt
    41041 ggcgcttgcg ttggcctttt tggtatcggt actttctctc aggtgaatgg tatctcatcg
    41101 gcagttcagg ctttttttga tcctaaaaag gaacatacag tgaatatttt tggcgctgac
    41161 tatacatggg ctgttataat ctctggactt ttgataacta tttttgctgc acttgttatt
    41221 atcggaggca taaagaggat ctcaagtgtt tctcaggtag ttgtgccttt tatggcggtc
    41281 gtttatatag ttgcttgtct ttcacttatt gttataaaca ttcagaggct ccctcacgct
    41341 gttgccctta tttttgaaca ggcattcagc ttcaaggctg tcgcaggcgg atctcttcct
    41401 acaatgttca ttgctataca aaagggtgtt gccagaggaa ttttctcaaa tgagtcaggt
    41461 cttggttcgg cacctatcgc agccgcagcc gctcagacaa aagagccggt aagacaaggt
    41521 cttgtttcta tgtgtggcac atttatcgac actatcattg tatgtactat gacaggtctt
    41581 gctatcgtaa tgatgggaag ctacgaggct atcaacccag taacaggcaa gccttttgag
    41641 ggcgttgagg tcacaaatca cgctttccag aacggcttct cgttcctgcc gtcaatgata
    41701 cctgcattta ttcttatggt ctgtcttgtg ttctttgctt tcacaactat ccttggctgg
    41761 aactattacg gtgaaaggtg tatggaatat ctttttaaca gcaacatgaa ggtggtcatg
    41821 gtctacagat atctctatat cctcgctgta tttatcggac catatatgac ggtttctgcc
    41881 gtttggacga tcgctgatat cttcaatgga cttatggcac tgccaaacct tatagcgttg
    41941 cttgctctca gcggtgttat cagtaaggat acaaggcgtt atgtggcgaa gtattccaga
    42001 gcaaaacgaa tcaataagct ggtaagagcg tagatatcgt attttatatg aagcggaaac
    42061 ggctgaatgt tgtttccgct ttttttatgc tgtaggctta atcaccaaaa aaatgaatgg
    42121 ttatttgttg aaatcaccaa aagtatttaa aagaatgtaa cagtgattgt aactttattg
    42181 acaacgatta cattcagtga tataatagct ttgtaagaat caaggaaacg ttttctaatg
    42241 aaaacgatct gttacggagg ttatatataa tgaaaaattc caagcgtgtt attgccggtg
    42301 caatggctct cttgtcagca gtgagcgtag cttcatgcgg cagctcatca ggcggcgatt
    42361 caagcagctc ttatgttgat aaggtaaagg tagcaagcac cgacgatatt gctactatcc
    42421 ctgatggttc ggaaaaggag ttggagtggc tttcttattt tgacataaac ccaactaaga
    42481 aagagccgga aaagagaacc gaccttactc tttttgagga aaagggcggt aagatcaagt
    42541 attctcagac atcatcaatg aacaagtatg ataagctcgc agcaagactt atgtctaacg
    42601 atccaccgga tatgttctgg tatgagcagt ctatgacatt ccctgcaaac tgtatcaagg
    42661 aaatgttcca gcctgttgat gatatcgttg atttcgacag tgctatgtgg agcgatgtca
    42721 aggacgttgc agagcagttc actctcaacg gcaagcactt tgttgcacct atcaacttcc
    42781 tgccaggttc tgttatcaca tatgataagt ctatgataga cgctgctggt cttgatgatc
    42841 catatgagct ttatcaggac ggcaagtggg attggaacgc atggtatgat atgatgtcag
    42901 aatatgttga gggcgctgct gctgacgagg aaagatacgg cataaacggt tggttcgcac
    42961 catttatctt ccagtcaaca ggtaagactc ttatcacata tgacgctgat aaggacgagt
    43021 atgtttcaaa tcttaacgac gctgactttg taagagcatc tgatatgctt tatgatatcg
    43081 caaagaatgg tatgtactat cctgattggg taggacaggc aggcgacgca ttcaagaaga
    43141 atatcctctt ctatgcaatg ggtccatggg cttctaccgg tacacattca ccaaaggacg
    43201 gcgacaattg gggcgtagtt ccaatgccaa aggatcctaa ctctgatacg ctttatacaa
    43261 ctatcgacat gaatgcgtat atgtgggtaa agggttcgac aaagaatgac gctatgaagt
    43321 gctggctcga gtgtgcaaag atagtttata ctcaggatac atataaggat atcgagaagg
    43381 agaagttctt tgtaaacaat cctaactgga cagaagatat gtataacgta gcttacgttg
    43441 accttgttac agacaagttc actaagatat ttgatcctgg ttatggcatc tccacaactc
    43501 tgtcagataa tgatgctgca acaaatgata ctaaggaagc tgttattcct tatctgtata
    43561 cttcagttat gaagacagat gagaatggct cacagtatac gtggacacag ctcaaagagc
    43621 agtataaggg tactgttgat tcagaactta aaactctcaa cgatcagtat cacgcatatt
    43681 tggaaaagaa taagtaatat aacttgccat ataaagacgg acggaattta tttcgcccgt
    43741 ctttttttgt tgtaatgtca gnnnnnnnnn naaaaaatgt gatataatca gagagtacat
    43801 taagatttaa ttaagaatta tgttgagctg tttttaaaga atgtggctta taataataaa
    43861 cgtaaaagaa aggatcgtgc cgatatggag ataaaggagc ctgtaatcct tgttgtagat
    43921 gatgataaag atatagtggg agctatcgca aacctgcttg aaatggaggg ctataaggta
    43981 ataaaagcct atgacggtct tgaggctttg gaaaagctca ctgagaacag catacagctt
    44041 atactcattg acgtcatgat gcctaagctg gacggacttt ctacaatgat gaaaataagg
    44101 gcaacagaaa atatccctat acttgtgctt tctgctaaat ctgaggatac agataaggtg
    44161 cttgggcttt caatgggcgc tgacgattac atcacaaagc cttataaccc tatggagctt
    44221 gtggcaaggg taagaagcaa cctgagaaga tatatgcagc ttggcgctat cgacacggca
    44281 aacaaaacgg atattataga aataggcggt ctggagcttg acactaaggc aaaaagctta
    44341 aaggttgacg gagaacctgt caagctcacc gccacggagc ttaaaattct tgagcttctt
    44401 atgaaaaacg caggcaggat attttctgcc gaggagatct attcaaaggt atggaacgag
    44461 gacgcatatg ccgttgaaaa cacggtaatg gtacatattc gtcacatcag agagaaaata
    44521 gaaataaacc ctagagagcc gaaatacttg aaggtggtgt ggggaattgg ctacaaaatc
    44581 gagaaaattt gagcaggcag aatggtttaa gatattctca gctaaaaaga tgagaattat
    44641 agcatttgtg cttgcctgcg ttattgcagg cggtttttgc ttgtctttgg tgctttatgc
    44701 taacagccat gctgacagca gcagtaagtc ttatcttaaa tcagaaagct ataagttggc
    44761 ttccaatgaa atgtatgagg aactgtgcgt tacagcttcg gcttatctca gatatgttga
    44821 tgaggatcgg aattacactc agtctagaca cctgctcaat gactatctga attatcttga
    44881 ttacaaccgc tataaataca aaaagacaga agacggtata cggcttgtag acagtcagtt
    44941 tgattactat gtggggatta ctaaaaatgg cgaaacaaga tatctgtcaa atataaaaga
    45001 tatagaaaat tatgacagcg aaacggataa gataaaagct tttaaatcgt cgcacaaaaa
    45061 ctatatactc cgccatgata acgaggtaat gagcgacaga acttcatcag gacagatagt
    45121 tgactactat tacaatacag cggagtatca ggataacgtg acagaagagt tttatgccga
    45181 caatcttgtg cccgtaggag gaagttatta tgataactac ggcagatatt tttacaatta
    45241 cggcaattat gatccaatag ctttctataa tgtagactca agatataatg ttattaaagg
    45301 tcttgatttg aaaaacggtc tgaaatattc cgacaggctt gaagatgaag aaattggtga
    45361 cgattatgac actgatgacg gcaatgaata ctatgactat gacaacggca tatattatta
    45421 tgaggacgat tacggaaaca catgggcaaa atgtgaaaac ctctatgacc tgcctgatta
    45481 caaagcgaca gacaatgacg attcaggtat aacggtattc tttgcaccga aagctgaaaa
    45541 gcttacggca atggaaaagg aatacaccga ggctttgaat gatatgtatc gtgcaaagct
    45601 ttttgtgatc gtttttggcg ttattgaatt tatgcttgtg tgctatctgc tactgatctg
    45661 tgcatataat gcagagcctg agagcgttga acgctggaag caaggcaatt ttctaaaaaa
    45721 agtgcctacg gaagtttacc ttatcctaat ggcattggct attacatttg cttttgtatt
    45781 tggcgcctgc tggaattatt atgtgagcat cacaaaaaat atttttgaca atgctttggc
    45841 taattccgtt ttcagaaata tcattgctgt agcgttattt gcattggcat attttgcagg
    45901 ctttggctcg cttatggagt ttgttggcaa gataaagaca agaagcatat tcaaagaaag
    45961 ccttatttgc agattatgta agttcctatg gcgcaagtat aaggaaacat cactgtatca
    46021 ctattttcat ggccttccac ttggagtgaa actatccata agaacgatag ccataggcgt
    46081 tatactgttt ttggaatccg cttattattc cagtgcagat gttgagccgt tcttgcttgt
    46141 tgtggctttg atactgatgg tgttagatat ccgtgacttc tcacagcttt caaagctcag
    46201 caaacagata gacagccttg gagaagaaaa gccttatgat ggcaagataa gaaaaacttc
    46261 gccggtgtat gctgaatctg aaaaactcaa ctcagttcag gataaggtga gagagtcggt
    46321 ggagcagcag atacagtctg agcgtttgaa aatagagctt gtgacaaacg tttcacacga
    46381 cctgaaaaca ccgctgactt ccattatcag ctatatcgac ctgcttaaga aaatggatct
    46441 taacgatgaa gcttcgagct atgtgaagat acttgataaa aaggcgcaga agctcaaagg
    46501 cattgtgtct gacgttttca gcatagcaaa agccacaagc ggcgttgatg taaacctaac
    46561 aaagcttgat tttgtgatgc ttttaaatca gtgtatcgca gacgcagagg acaagatagc
    46621 tgacagtggc aagatagtaa agataaacat aaatgccgac agcgcaatgg taatgggcga
    46681 cggcgacaag ctttacagag tgttccaaaa cattgttgac aacgctctga attactctat
    46741 ggacggcaca aggatattcc ttgatgtgta caaaaaagcg gatagaatag tatttgccga
    46801 gaagaacatt tcggcaacgc cgataaactt cacagaggaa gagatacttg aaagatttgt
    46861 gcgaggggat aagtcacgaa cagacggcgg aagcggtttg ggattatcca tatcaaagag
    46921 cttcacggaa gcctgcggtg gtatcttcaa tatcgaaata gacggagata tgttcaaggc
    46981 gatagtcgaa atgccgataa ttcaggagga tacggcagag aaaaccgatg acaaaacgga
    47041 atagcgtaaa taataaaacg gtggagaatg acaaacactc tccaccgttt ttacatatac
    47101 gcaattacta tgcgtgagat aaaatatctg cgttatttta tcccctttac cttataccct
    47161 gcgtccgtta ccgccttaga aagcacctcg tcagccacgt ccttgtcaag tgtgatgtaa
    47221 gcgcacttgt cctcaaggga aacatctgct gtaacgccgt ctatcgcatt aagcaccttc
    47281 gatactgtgc ctgtgcagtg tgaacacatc atgccgtcga tcttcattac ctttttcatt
    47341 tttccgtcct ccgttttagt atttatttca gcctttgcgg ctttgttttc tctgccactt
    47401 ttaaaaagat tgagccttaa cgcattggtc acaacgcaaa ctgagcttaa actcattgcc
    47461 gctgcaccga acacaggatt gagcttccag ccaagcaagc cgtagaatac tcctgccgcc
    47521 agcggaatac caagcgagtt gtaaataagc gcccagaaaa ggttttcctt gatgttcttc
    47581 ataacgctcc tgctgagctt gacagccgta acagcatcat tcagatcgct tttcattagc
    47641 acgatatcag ccgactcgat agcaatatcc tgtcctgcac cgatagctat tccaacatct
    47701 gccgttgtaa gggcaggtgc gtcatttatt ccgtcaccta tcatggctac ctttttgccc
    47761 tgctcctgca attctttgat tatcgtagcc ttgtcctcag gcataagctg tgatcttacc
    47821 tgtgaaatgc caagcttttt ctgtattgct ctagccgttt tctcgttgtc ccctgtgagc
    47881 attacagttt caatgcctgc ctttttgaag ccctctatag cctctgcact tgtctctttt
    47941 atcttgtctg caatgaacag aatgcctgca agcttgttgt cgatcgctac ataaagtggt
    48001 atctcaccgt tgtcagcgtg agcatgagcc gtctcctcaa ccattgatat gtcaactccg
    48061 ctgttatcca taagccgctt gttaccgata agaacgtttc tgccctcaac aacagccgat
    48121 ataccaccgc ctgctgtttc tgtgtagctt tcagcacggg agagttcaat gccatttgtc
    48181 ttgcagtggc ctgtaacagc ctttgcaagt gggtgtgatg aaccgctttc tgccgagcca
    48241 caaagctttt taagctctga aacatcaaaa tcaccaaacg cttgcgctga aataagctca
    48301 ggcttgcctt ctgtgcaagt tcctgttttg tcaagaacaa cagtgtcaat gctgtgagca
    48361 gtttcaaggc tttcagcgga ttttattagg ataccgaact gtgccgcttt acctgttcct
    48421 accattattg cagttggcgt tgcaagcccc aatgagcatg ggcaggatat aacaagcact
    48481 gatattgcca tgttaagagc aaatgcgaag ctatagccaa gtagcagcca tactaccagt
    48541 gaaataagag ctatcgttat aactataggc acgaacacag cgcttatctt gtctgcaagc
    48601 cttgctacag gcgcttttga agcggaagcg tcctcaacga gtgcgattat ctgtgaaagt
    48661 gttgagtctt tagcttgctt ctcacacctt accttagcaa aaccgccagc cgatacgctt
    48721 gcactcataa ctctgtcacc cactgccttg tctacaggta tgctctcgcc tgtgagtgca
    48781 gattggtcaa ccgtgcagtt tccatcaata agaactccgt cacatggcac agaccagcca
    48841 gctttgagca ggaatacatc tcccaccatt acgttctccg caggaacttc catctcctcg
    48901 ctgtcacgga taacaactgc cgtcttaggc gcaaggctca cgagcttatt cacagcatta
    48961 gccgtcttgt cttttgagcg agcctcaaga tattttccca cagtgataag tgtgagtatc
    49021 atgccgcatg actcataata tagattcatc atatactcat gtgccgagcc aaggtcgccc
    49081 ctgcccataa agtaagccat tctgtaagtg ccgtaaacac tgtatatgaa tgaagccgcc
    49141 gcaccaagag ctataaggct gtccatgtta ggggagcggt ggataagatt tttgaagccg
    49201 tttgaaaaat agtgaaagtt tattgcaatg ataggcactg tgagcaaaag ctgagtgagt
    49261 gcgtatatca tcatgttttt gtgccctgta aaaatagggg ggataggtat gttcgccatg
    49321 tgtcccatgc aaatatagaa cagcggtatc aaaaatccca cagaccacca taggcgtttc
    49381 ttcatctttt tactttcgtc ctgagccgcc gcaacaggtg atgcagtttt gttttctttg
    49441 tcgcctatct cgcttgctcc atatcctgca ctttcaacag catttatgac cgcctgagcg
    49501 ttagttacgc cctcgtcaag atccaccgtc attttgtttg caagcaagtt cacgtttacg
    49561 tccatcacgc catttacttt actcacagcc ttttccacat gagcctgaca cgccgcacaa
    49621 ctcatgccag ttacattgaa ttttaacttt ttcatagata tttgcctttc tatttaagct
    49681 ttttcataag ctctaatact tcatcgacaa cttcagtgtt gccgctttgt accttctctg
    49741 tcatgcaggt tttcatgtgt tcctgcaaaa taagatagcc gaagctttgc attgcacttt
    49801 caacagctgc aacctgaatg agtatatctc cgcagtagcg gttatcgtca agcattttga
    49861 taatgccgtt tatttgtccc gaaactctgc ttagcctatt ctttagctgt tttatttcct
    49921 tatcatcacg gggagtgtgc ttgtgcctgc aagtatcaca gcagccgcat gacagttctt
    49981 tatcatttct gttgtccatg ttttttttgc ctccttacta tatcaaatac ccttatgggg
    50041 tatcttttgc tgcatatata ttatataccc cttgtgggta tttgtcaacc atttttttat
    50101 attttttgca cgaagcaaaa aaatatccgc cctcccaaaa ggaaagcgga tatcaattat
    50161 tcagttagta gccgtaatac ccagtgtcat agctgtagtt gtaagcattt tcttcctgat
    50221 actgcttgat agcttcgtca atgtttttac aactgccgta aactatatat tgaatgaacg
    50281 cagagtttgc ggcaaagtcc ggattaagca cgtccatgct atagatgact tcgtttgtga
    50341 aatagccgtc agcaggtatt ctaagctgct ggatatcata tttcatgtag tcaaaagcat
    50401 tgaggagcag atttgcgatc tcaccatcag taaggtctgt gtttatctca ggaaggactt
    50461 tgttcagaag cttgtcaagc tgaacaagac tgagcttctt ggattcggtt atgatctctg
    50521 agataacacg tctttgacgt tcggtacgct catagtcgga atttcctaca tatctaagcc
    50581 ttgcaaacgc aagtgcctga tttccgttaa gatgaagctt taccttatcc tcatcactct
    50641 tgcccgcaaa tatgtcgtcg cttatatagt cagtgccttt cttattttta aggtatttgt
    50701 tctgttcggc catcggcttc ttcatgccct cggcttcagc ctttgacact tccatgtcga
    50761 taccacctac agtctcaact atatctataa acgagtagaa attcactctt gcgtatctgt
    50821 ctatctggat accgaaataa tcctcaatac actgttcaag atactcacag ccaccatgcc
    50881 agtaagcgct gttgagcctg ttgccctcgt caaactgcgc catgtaaatg tacatatcac
    50941 gcataaggga ggtcattgta atagtcttgt tcttggtgtt tatggaaatt atcatcataa
    51001 catcagtatt tccacggctc tcctcttctg tatcacggat atcctcgcca atgagcagaa
    51061 tgtttgttac gtcctcgctg gatatcttgg aggactgctt ttgtagctga gccttaagct
    51121 cgtcctcttt ttcctttgcg tcaaatgtat ccgcctcact tgtaagctcg ctggaattta
    51181 taggcgctct gcctgaattt atcttagtat ctttagttct gtcaagcatt cctgtgtagt
    51241 ggaaaaacaa acctacgata atggcaatta taataatgaa tactacaaat attattccaa
    51301 gagcaatacc cgtctttttc ttcttgctcc agcctctttt cttttttggc gtcttatcct
    51361 gctttgaatc agactcatgt gtcggatcag ctttctgctg ttcctttgag gggataggta
    51421 tgacccccgt aggtccgttt gagtttgaag catcaagtat ctttctattt tctattgcat
    51481 tggatattag cgactgtaaa tccttatctc cctgagtgtt ggaggtgttc ttgttgtcgt
    51541 tgtccataaa ataaacctcg tttccgatta aagtcagcaa gccggaagtc tatagctcgt
    51601 ttaagcataa tacaataata gtatatacta ttttacctca aattacaaca taaatttcgt
    51661 acgaatttga ctttaaaatt acatcaattt ttataaaatc aaataaaacc gctttaattt
    51721 acaacaattt tacaagagat tattgcagc
//
LOCUS       DAWEMR010000004        43227 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_367_length_43227_cov_13.056124, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000004 DAWEMR010000000
VERSION     DAWEMR010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 43227)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 43227)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..43227
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_367_length_43227_cov_13.056124"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4976..4985
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    25365..25374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    34823..34832
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    41318..41327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        12549 a         9142 c         9962 g        11534 t
ORIGIN      
        1 actcttgatg aatttaaaca tttagagaat ttttcacaag ttttagggcg ttagttaata
       61 ttatgtgtct attcttgaaa atatcttgca aaaattaaat cataaaacaa taattcagtg
      121 gagataactt tcagaaattt acagatatga gatgaggcgg ctttgatatt atcaggcggc
      181 tatgtataaa tttaaccaca aaggagtaaa attttatgtc aaaaaatatt tttttgtttt
      241 caggtcaggg ctcacagtat gtaggaatgg ctaaggagct ttgcgaaaag tatgatgctg
      301 caaatgcggt tttcgacaca gcggaaaagg tgctgggata tgacttgaga gcggtaacat
      361 ttgacggtcc tgacactgag cttaacaaga cagtgaactc acagcctgct ataatggctt
      421 gctcgcttgc cgcttttgag gctgcaaagg ctgagggcat cacatttgac ggcgtagcag
      481 gtcactcact gggcgaatat gctgctatgg tagccgcagg tgttgtaact attgaggacg
      541 gcttcaagct catcaaggcg agagcggctg caatgcagaa agccgcagag gctaacagtg
      601 gctcaatgta tgctataatc ggtctgcctg ctgaagaggt cgagaagata tgcgaggaaa
      661 cagagggcta tgttgttcct gtaaactaca actcagccgt tcagactgtt atcgctggtg
      721 atactgcggc ttgcgaggcg gctgcggcaa agtttgcgga gatgaagaaa aaggctataa
      781 agctcaatgt tgcgtccgct ttccactcaa agctcatgca gagtgcggct gatgaattta
      841 tcgaaactgc aaagacagtt gaattcaaga ctgcaaatgt agatttttac tcaaacgttc
      901 ttggaacaaa gcttgaggac ttttctgcta tgcctgatat gcttgccaag cacatcgttt
      961 cacccgttaa gttcacttct gagcttgcag aaatggaaaa ggcaggctat gaaaacttta
     1021 tcgagcttgg tcctaacaag gtgcttacgg gtcttgtaaa gaaaactctc aagggcaaga
     1081 acgctgtgaa cattgagaac aatgccacaa tggaaaaggc tcttgcttct ctgagctaaa
     1141 atatcgaggg gagaaaaatg aaaaaggcta tgaaaagggc tttgctgttg gcattggcgg
     1201 tggcaatgct tgtttgtatg agtgcctgtg gaaaatgtgc gtggaagata aagataaacg
     1261 gcaaggatat acagatacca tgcactctgg acgatattgg cgaggaatat gaatacacgc
     1321 tggattatcc tttttcggga aactcaaacg gcaaggggat attctcggtg gcgcttatgc
     1381 aggacggcag tataataggc actgttaagg tagaagcgga caatgttgac gatattgaca
     1441 ggaagtcgaa gatacgtcag ataagtgcgg cgcaaagcag ttccgataaa aagggaatga
     1501 gcattgcagg ggtaaaatgc ggtgatgaca aggcggaagt caaaaaagtt ttcggcgagc
     1561 ctgacagtcc tgacgcagac gcatggaaat acaagaaaag gggcttttta gcgactttct
     1621 tttttgatga tgacggcaag gtgacaatgc tgagcatatc agacattgat gatgaagata
     1681 cctgataaaa ggcggtgtat aaaatgaaac gcaatgacta catatcatgg gacgaatatt
     1741 ttatgggcat ttcactgcta tctgcacaga ggagcaagga ttctcacacg caggtaggtg
     1801 cgtgcatagt ttctcaggac aacaagatat tgtcagtagg ctacaacggt atgcctaccg
     1861 ggtgttctga tgatgagatg ccgtgggaca ggaacggaga gtttcttgac acaaaatatc
     1921 catttgtgtg tcatgccgag cttaacgcca ttctcaacca tgcggcagga agcttacaga
     1981 atgccaagat atatgtttca ctttttccat gcaacgaatg tgcaaaggct atcattcaaa
     2041 gcggcataaa ggaagtcgtt tactgggagg acaaatacgc tgacactgac ggagtcaagg
     2101 cttctaagaa aatgtttgag ctgacggggg taaagatacg caggtatata cctactaacc
     2161 gtgagataac tataaggctg taaaaggcaa ttatcctgcc gaaacagttt caggcgacaa
     2221 tgcgggcgaa cactgttcgc ccctactgta cattgtgcgg ttattaaggt ttgtgcaata
     2281 aattgcaagt tgcccctaca gatatacatt attaatatgc ggaaattttt tataaagaag
     2341 gaatgtaaaa tgtcaagaaa atacgctata cttggagaaa cgcttaaaca cactatgtcg
     2401 cctcctattc acgctaggct ttttgagctg aaaaaccgtg aatacgagta cgagatactg
     2461 gaggtaaagc ctgaggagct taaggacaag gcggagtatc ttaattctct taacggctac
     2521 aacatcacta ttccccacaa gataggcatt atcgactaca ctgatgagct tgaccaatcg
     2581 gcaaagcgtt atcactctgt aaactgcgtt gacaacaaaa acggcaggca cgttggttac
     2641 aacactgact gcgacggctt tctcagaact gttcgtgcaa tgaatatcga cttcacggga
     2701 aagatattac ttataggctg cggcggagta ggcagaatga tggcaattga ggcggctctt
     2761 gccggggctg acctttacat tgcggtactg gagagcgata tgcctttggc taaacaggta
     2821 gaaaaagaga taaagactat gaagtctgac gctaggataa ctattgttct aaacactgag
     2881 ataccgaccg acgttcacta cgagctgctt atgaacgctt gccctgtggg aatgtatcct
     2941 aagacaaacg cctgccctgt tcctgatgaa gttataaagg cgagcggtgc ggtatttgat
     3001 gttatataca atcctcgtga gactttgctc atgcagaaag ccaaggctat gggcaagaac
     3061 gctgtcggag gcatggcaat gctagtttgg caggcggttt ccgctcacga gatatgggac
     3121 ggtgacgaat acacagatga ggaagttcag tctatcatag acgaaatgga gatcgctgtg
     3181 gagaaggatt ttaaataact tgcgatactg acatatgaaa atgtcaaaaa caaggcttat
     3241 cgagtaaaaa cactgtgcgc taggagaagc gggcgaacgc tgttcgcccc tacatgaaac
     3301 acggtgtttg cttgattgca gagatgacgg acaaacagtg acagtgggcg accagagatc
     3361 gcccctacat tagtttggca aaatacatta taataaggat agtaaatatg agaccaatat
     3421 ttttgtgcgg ctttatgggc tgcggaaaat ctactgtggg aaagatcttg gcgaaaaaaa
     3481 tgggctgtca gtgcgttgac cttgacaagt atatcgaaga tacgtcagga atgagcatac
     3541 ctgaaatatt cgacaaatac ggtgaggagc atttcaggaa gcttgaaacc gaggctcttg
     3601 cagcatttgc tgacattggc ggcgttgttg caacaggcgg cggagcgttg ctttcggagg
     3661 agaacggcaa ggtcgctaaa agatcgggca tggtggtctt tatcgacaca tatttcaaca
     3721 cctgctacgg acgcataaaa aacgacccta acagacctat cgcttacaac tccaccagag
     3781 aacagctttt agagcggttt gattatcgaa gacctttata tcttgcccac tcacactatg
     3841 tcataagcgg tggttatccg cctatcgtca tcgcttctaa gatacaaaag ctttacaaga
     3901 gatttgattt cgggcagggc tgatttagct ctttcctgca ggctcgacct tcatgcccac
     3961 tgttttcgag cttatcgcaa accatgggag ccttaagctt atgcccgttt atcttgcatt
     4021 tttcttcata cttctgcttg tgatgatatc aaaaacggaa aagctttgtc ataataaata
     4081 gtacggcttg tgcaaacagt accaaattat ttgtatcttg gataatcaaa aatagctatt
     4141 tataacgaat aatccaaaaa gtctgcaagc actgataaaa aatgttgaaa atccttccga
     4201 catgttgtat acttatggta tgaaagcgtt atattaaact gccatatgac agaagggagc
     4261 tgcaaatgga catacagaaa accgtgcttg tcgttgacga cagcacaacg aaccgatcta
     4321 tactttgcga catactacat tctgactata ctgtgatcca agcagaaaat ggcatacagg
     4381 ctcttacaaa gctaaaaaga caggataaaa aagttgacgc tataatactg gatatcataa
     4441 tgcccgaaat ggacggatat gagttcatga ataaaataaa gcaggataaa accttatctc
     4501 agatacctgt tattattcta acagaaaaat cagacagaga cactgaaaag aaggtgcttg
     4561 aaagcggtgc gtgggacttt gttccaaagc cttatgacgc tgatattata aagctaaggc
     4621 tcaaaaacgt tatcgcaaga agtcaggtat cgttgctcaa agagcttaat aacgttatga
     4681 actacgaccc tcttacggag atatattcaa agaataaatt tttcagtgca tcaaaggcac
     4741 ttcttaagga taatcctgac aagcagttcg cttttctaag actagatatc gaccgtttca
     4801 agcttataaa ttcatttttc ggcactgcgt atggagacag actgctaaag cgtgttgcaa
     4861 aacgtataag agattttgca aaaacaacgg aatgctgtac ctttggtagg atagacgctg
     4921 acgttttcgg tatcttcacc ccttatcagg gcaaggaaga aacagtaaag cagatnnnnn
     4981 nnnnnatgat cgtttacggt gtttatgtgg tgactgacag aagcctgcca atatccttca
     5041 tgtgcgatcg tgccgctctt gccgctaaaa ctgtaaaagg gcattatatg aaaagctatg
     5101 ctttttatga tgacaaaatg cggctttctc tcgaaaatga gcagaatata ataaatgaaa
     5161 tgagcgacgc tcttgaaaac cacgagtttg tgccttacta tcagcctaag tatgatgtaa
     5221 agacaaacaa gcctgtgggg gcggaggctc ttgcaaggtg gatacaccct accaagggat
     5281 ttatctcacc tggtgtgttt atcccgatat ttgagaagaa cggctttatc tcaaagcttg
     5341 atttctacat atgggaatgc gtttgcaaac agctcaagga atggaaagac aagggagtac
     5401 cactttttcc tgtgtctgta aacgtttcac gggtgaacct ttacaaccct aatctttcaa
     5461 aaataataat cgaacttacg aaaaagtatg acgttgaccc taagtatttc aacattgaga
     5521 taacggaaag cgtttatact gacgacaatg taatgataga tgatatcacc agtcagctca
     5581 gaaataatgg ctttactatc ctcatggacg attttggcag cggctattct tccctgaacg
     5641 tactcaaaga tgttcaggtg gatatgctaa aaatggacat gatgttcatg ttcaaggcaa
     5701 agtatgacgg ccgtgcagaa actataattt cttctgttat cagaatggcc aaatggctca
     5761 atatccctgt tatcgcagag ggcgttgaca gagcggagca ggtagaattt ctgaaaagcg
     5821 taggctgtga ctacattcag ggtttctatt attcaaagcc tttgcctgcg gcagactatg
     5881 aaaagcttat ttcagatcag gaggaacagc ttgtgcctga gaacagaacg tcagtaaacg
     5941 acctgctgtg gggcaagagc ggaggattgc tggcatatat cgacagcatc gaccagcctg
     6001 ctacgatata tgaatgtttt cccaacggca cagtggtaat ggtatgttca aacaaggcat
     6061 tttcacaaaa atacggctat ggcagcagta tttacggcaa actcaatgtt aagaactgca
     6121 ttgacagcga atgcaaagag aaattcacaa gctctgtgaa caaggcaata gagagcaagg
     6181 acagagcaga atgtgttctc agtatgcacg agataaacgg caagaagcac tggttcaaga
     6241 cgcatttacg atttatcagc gaaacaggtg taagctctgt gcttatagcg tatttcacgg
     6301 acgtgaccga catggtgttg acggataaac gcataaacga atataagaac tatatccaag
     6361 atgaaataga ccgaaagcac aagatcctta tcgtcagcaa caacagcgac acatgctctc
     6421 agctcgaaga gatactttct caggaaaata ctgttttcac tgcagaaacc attcaggcag
     6481 gaaaaaagtt tcttcttaac gaggatatcg accttattta ttttgacata cagcttattt
     6541 ccaaggacga cgaatttcct cttgacattg atgagagaag actccccgtt attgctataa
     6601 ctcaggctca tagcgtactt aaaggcatga caaaattgaa gaacagagtt tcagactttg
     6661 ttatgaagcc gtatatagat gagctggtac gcctgagaac gaacaatctt ttaaagatca
     6721 atatttcagg cagtgcaaac gaaaaatatt ttagccggac gaaatgattt gaaaaagtcc
     6781 tctgcgataa gcgggggctt ttttctctta ttaagctgtc agctttattg acaaaatata
     6841 cactatctga tattatatag gtatgaatgc tgaaagcgag ggagaaaaaa tgtatcttga
     6901 caagtggcgt gtggcaataa gaaatttgaa aaattcgctt ttttcatatc caaacacggc
     6961 tcaaagctgt ttccctgacc ctatcacgga cgacacagtg ttttactgcg ttcaaggtct
     7021 tgtatatggc agtcaaaagt tccctgacgg aaaattcata cgcagttctc ctgtgacctc
     7081 ggtatactcc gaggacggaa agctttttgc caaaacaata aacaacgttt atgagcttaa
     7141 aacgccaagc gattattttg actgcgatat tgcggaaaat gccgcacaaa aaacaggaag
     7201 cagtgagctt gctgaaacag ctaaaatgca gagagaaaag ctcatggcac agcttgataa
     7261 atttttcacg atctgccaga ggctttgtga taatgaacag gctgacgttg caaatgagtt
     7321 acctgaagat actcttgtta tatgctatta cggcactgac ccacatggaa acaagatgtt
     7381 ctacaaaaat gaagatatgg tagtgcctgt ttttgcatat gtgaccgttg ggctggtcat
     7441 tgattctgtg gggcttgcgg aaagcaagga aaagctggat ttatgggata cgctggggag
     7501 atattatatc gacagcgagc ttatggagtt ttatgactgt gacacgaaaa accttatttt
     7561 ctgcaatttg acgaactctc ctatatatgt cacaatttat gaaagctccg ccgctatgct
     7621 ctgcatgccg aacacagcta agatagcttc aagaaaagta aaagaaaaat aaaactttta
     7681 tccgtctgag atctcaggcg gatttttttc attgcacaat atagagaaga acgttgaaaa
     7741 ttaatagaat gttaaacttt taacgtgtca tagcaattga ataatcgtcg aaagtgtagt
     7801 ataattttct tatatagttg ttttttgatg taaaataata tatttaacta ttttttgtat
     7861 cgtgaggaaa agatcatgga aattttaaaa cagcgtattc ttaaggacgg agtagtaaag
     7921 gaaggaaacg ttctcaaagt cgataaattc cttaaccacc aaatggacgt aaagcttttc
     7981 aacgatatcg gcgaggagtt tgcaaagaga tttgcaggaa caggcgtaaa caagatactc
     8041 actatcgagt catccggtat cggcattgcc tgcattgcgg ctcagcattt taacaactgc
     8101 cctgttattt ttgcaaagaa aactctcagc aaaaatcttg acggcgaaat gttcgttact
     8161 caagtaaagt cctacacaaa gggcgttaca tatgacgtaa tggtatctga aaaatatctc
     8221 ggcaagggtg acaaggtgct tattatagac gattttcttg ctaacggctg tgcgcttatg
     8281 ggacttatag atattgtaaa gcagtcaggg gctgagcttg cgggagcagg tatcgttatc
     8341 gaaaagggct tccaggtcgg cggaaagact atccgtgaca tgggcgtaaa gctcgaatct
     8401 cttgctatcg ttgactctat ggacaacggc actatcactt tcagggacta acgcacaagg
     8461 cgttaccaat aaatatttta tggaggaaga tcaaacatga aggtaagacg tattcttgcg
     8521 ctcgttcttt ctgcggctct tgctgtaaca gctcttacag cttgtacatc tgctaaggac
     8581 gaagcatcac agaagtcggc tgacaagcag acgacaactc tcagctatga cactgtaaag
     8641 atcggtgtta tacacatcgg caaccctgcc gacggttcag gctacacata tgctcacgat
     8701 cagggtatcg ttggtatgca gaaggagctt ggacttaaag acgatcagat cgtaagaaaa
     8761 aacaacgtat cagacagcga ccctgctgct acagaaacag ctatccgtga gtgcatcgag
     8821 gaaggctgca acatcatctt cggcacaagc tggggctata tggacaaaat ggaatccctc
     8881 gctgaggaat atcctgacgt ttatttctgt cacggcacag gctacaagaa caacggcaag
     8941 aaccttaaca actacttcgg aagaatttat caggcaagat atctcaccgg tatcgttgca
     9001 ggtatgaaga cagagtcaaa tcttatcggt tacgtttccg ctatgggcag cgagaactct
     9061 gagtgcacag gcggtatcga cgctttcgca atgggcgttt attctgtaaa tcctgacgct
     9121 aaggtttatg taaaggtaac aaacagctgg tattcaccaa cagaggaaac aaacgctgca
     9181 aaggctcttc tttctgaggg ctgcgacgtt atcgctcagc actgtgatac acctaaccca
     9241 cagctcgagg ctgaaagcgc aggcaagtgg ggcgtaggct acaacacaga tatgtctaag
     9301 gacgctccta aggctacact tacttcaact ctttggaact ggtctgcata ctacacatat
     9361 caggtaaagc agatcgtaga tggcaactgg aagtgtgaga actactacgg cggcatggct
     9421 gacggtcttg tagacatcgc tcctctgaac aaggatctct gcacagagga tatgcagaag
     9481 gctgttgacg aggctaaggc taagctcgaa gacggctcat tcaacgtatt tgacggcgtt
     9541 atcgaaacaa acgacggcaa aacagttggc gaagagggca aaactcttga cgacgctact
     9601 atcacaggcg gaatcaactg gtacttcaag aacgttgagg tcaagtaata aggcttaaca
     9661 gcttaacaaa acatttatac gggcggtgca ggttgccgcc tgttttgtta atgatatgag
     9721 catacggcag caggtaattc tgctgtcggt ttctgatgac attagcaaat ctaaggaagt
     9781 tcataaaaag gagctgaaag tatgagtgaa gaaatatctg ctgagatcgc tgtaaaatgc
     9841 agcggtataa caaaagtttt cggcagcgtg gttgcaaacg acggcattga tcttgaagta
     9901 aaatacggtg agatacttgc actgctgggc gaaaacggat caggaaagac cacgttgatg
     9961 aatatgcttt cgggtatcta tcaccctgac aagggcaata tctacattgc cggcaagcct
    10021 gaggcgataa attcacctat ggattctatc gaacttggca taggcatgat acatcagcac
    10081 ttcaagcttg tggaggtgtt ctccgccgca gacaacattg tggcaggcac aaagggcaag
    10141 cttaagagca agggcgctgt gagagatgag ataaaaaaga tatctgaaca gcttggtctt
    10201 gaagttgacc ctgacaaaaa ggtatacaat atgtccgtca gtgaaaagca gacagttgag
    10261 atactgaaag tgctttacag gggtgcgaag atacttattc ttgacgagcc tacggcggtt
    10321 cttactccgc aggaaaccga aaagcttttc aatatcctga gaaaaatgaa agctcagggc
    10381 tgtgctatca ttatcatcac ccacaagctg ggcgaggtaa tggatatatc tgacagagtg
    10441 accatactgc gaaagggcaa gagcgttgaa actgtaaaca cagcagagag cagtgaaaag
    10501 cagctcactg agcttatggt gggcaaggcg gtggagcttt caatagaaag acctgaacct
    10561 gtgaacgtaa agccgatact taacgtagtc gatctgacgg taaagaactc tgagggaagc
    10621 attgctctcg atgacgtaag ctttgttata aacagaggcg agatacttgg tgtggcaggc
    10681 attgcaggaa gcggtcagaa agagctttgc gagactatcg cagggcttca gaagatagaa
    10741 aagggcgcag tgctttacaa caaggagaac atcataggca agacacctaa ggagataata
    10801 aatctcggca taagcatgag ctttatccct gaggacagac ttggaatggg tcttatcgct
    10861 tcggtgggta tgacggacaa tatgcttctt aaaacataca agcagggcaa gctttgcttt
    10921 gtggacagaa agcctgctaa ggagctttca aaggagctta tagaaaagct tgaaataaaa
    10981 actccgtcaa cggagcttcc tgtaagaatg ctttcgggcg gaaacgttca gaaggtcctg
    11041 cttggacgag agatacacag tgagcctgac ctgctcataa ccgcatatcc tgttcgtgga
    11101 cttgacatca actcatcata tcttatatac tcgcttctta acgagcagaa gaaaaagaac
    11161 acggctgtgc ttttcatagg cgaggacctt gacgttcttc tggagctttg cgacaggata
    11221 atggtgcttt gtcacggcaa gataacaggt atttgtgatg caaagacggt cacaaaggaa
    11281 caggtgggca tgatgatgac aggctcgaca atggaggagg tgctgggcat tggctgatat
    11341 tataaaaaaa gagcctctta taagaatagc caagcgtgct gaacgctcaa acaagcaaaa
    11401 gcttatcata aggcttctgt cggtgcttct ggctatttgt gcaggcggaa tattcattct
    11461 tattcttggc aaagatccgt tttcatacta tgcgaaaatg gtaaagggtg cttttatcgg
    11521 caagaacgcc gcaagccctt ttatgccgtt caaagagaca ataaagataa tgataccgct
    11581 tcttatatct tctcttggca ttacgcttgc gtttaagatg aagttttgga atatcggtgc
    11641 agagggtcag ataataatgg gcggagtttt cgcttcatac tttgctcttt atcattacga
    11701 tatgcctcac tatctgcttt tcattgtgat gttcgttgcg gcgatgatag gcggaggact
    11761 ttacggcatt atccctgcat ttttcaaggc aaaattcgac acaaacgaaa ctttgctcac
    11821 ccttatgctc aactatatcg cactttacgt tatagattgg ctcacaaagg gcgcatggca
    11881 ggatcctgag tcaaagggat atcctaagat agcaagattc gaccttaacg catggcttga
    11941 ctcgctgggc ggtgtgcaga taggctggtt gataggtctg gtgcttgtgg tattggtatt
    12001 tatctatctt aaatacacaa agcagggcta cgagataact gtagtatcag acagccacgc
    12061 aactgcaaga tatgcaggca tgagccctag caagataatg ataagaacga tgtttttgag
    12121 cggtgctatc tgcggtatcg caggaatggt gcaggcttgc ggaacagaac acacactttc
    12181 aacatctgtt gcaggcggag taggcttcac ggctatcata gttgcatggc ttgcaaacct
    12241 caatccattc ggcatacttg tggtgtcatt cttattcgca gttttggaaa agggcagcac
    12301 ggtggtatcg tctgaatttg ttctttcacc ttactgctcg gacgttttgc agggaataat
    12361 cctgttcttc gtgcttggct gtgagttctt catcagatac agatttgtaa caagaggagg
    12421 caggaaaaat gactagactt atttcattcc ttgcccatgc agtatatatg ggtactcctc
    12481 tgcttttcgg cacagtagga gagatacaaa acgaaaaggc gggacacctt aaccttggcg
    12541 ttgagggaac aatggcaatg ggcgcttgcg caggctttat ggtagcatac aagacaggaa
    12601 gcctttggct ttccatactt gcggcatttc ttatgggtgc gcttgtttcc cttatctatg
    12661 cttttcttac cataacaatg atggcgaacc aaaacgtaac aggtcttaca ctgactatat
    12721 tcggcactgg actttcaaac ttcataggcg agcagatgat atccgcttca cctacaggca
    12781 acctcaaact gcctgacgga gtgatgagca gtctgaaaaa cattgacata ccgcttctca
    12841 gcaagatacc tgttcttgga gaggtgcttt tctcacagaa cattttcaca tatctgagca
    12901 ttgttatcgc tgtgataatg tttatctatc tgaaaaagac taaggtgggt cttaacctta
    12961 gaactatcgg tgagaatcct gctgttgcgg acgctgtggg aataagcgtt gtgaaataca
    13021 aatacataaa cgttcttatc ggcggaggaa tatgcggaat aggcggagcg ttctgctcaa
    13081 tggtaataaa cggcggtgtc tggatggcaa acagcgtgaa cggtcttggc tggatagctg
    13141 tggcgcttgt tatcttcgct gtatggaatc ctgcaagggc tatttacgga gcgtatattt
    13201 tcggtgcatt catggtgctg aaatactact tcccgaaaaa cctcggcttc actgttcctg
    13261 agtcattctt tgatatgctc ccatatcttg taacggctct tgttcttatc atcacttcag
    13321 tgaaaaaatc aagagagcgt tcacagccac aatcctgcgg tataaattat ttccgtgagg
    13381 agagataaaa cgtgacgtaa cgggcgaact gttcgccccg agaatattga aaaagtatca
    13441 tatttaaaag ctctgcattt tgcagggctt ttatttttgc cctagtgaca aaatgcagaa
    13501 gatgttcata gcccaattac gatacgacaa aagaaatggg cgaccacagg tcgcccatac
    13561 actttgttag gttatgataa actgttttac ttcgcctttg ccgaagccac aaaagatttg
    13621 agctcctcct caggcatata gccgacagtt gaagcaacta ccttgccatg tttcacaaca
    13681 aaaagggcag gtatgctcga gataccgaaa gcggatgcaa gctcgttttc ctcatcaacg
    13741 ttgaccttcc ccacctttac agaactgtcc tcctcagaat acttatccac cagcggtgaa
    13801 agcattttgc acggaccaca ccatgctgcc cagaagtcaa gcaggacagg cttttccgag
    13861 tgcattactt cgttttcaaa attatccttt gttatgacct ttacagacat ttttcacagc
    13921 tccttttcta gtccttgctt ttgtaataga gcatacaatg gcagaagccc tcaaaattcg
    13981 ggtcggctat ctgctcacgg aactctttgc acatacactt gttttcctct gtgcgctgta
    14041 tcctgcacgg acaatagcca cctgtgcgtt taagcccctc ttttatgctc tttaccattt
    14101 ctttatcatc attaagtcgt accattacta cctatccttt gcagatatgt ttagattatt
    14161 ccttgacaaa tgcctttact atctcgccga tatactgata gtgataatcc ttgtcagaat
    14221 accatttctc atcgttttca gtatcaacaa agcactcagg tctcatctcc tgaacataag
    14281 cctttttgca cacaagcacc atatcagctt ccgcaaaagc tgttacctcc acgccgtttt
    14341 cagacacgaa cacaggggtc agacctgttt cctttgcctt gtcgcagtca cgtccactat
    14401 gtgagccaca gaatgatagt gcctttttat actcctcttt gaaaaagctt atggtgaaat
    14461 attctgcctt gtcgataaat tcctttgtat atctctgcgg tcttataact gtggtgaagg
    14521 tgttctttcc ccacataacg cctgcaaagc cccatgaagc tgtcattgtg ttccagccac
    14581 tctcgtcgcc tgctgttaca agaaaccact ccttgcctat ctttttgtat ggattgaagt
    14641 caatagcgtc aagcttttct gttacctctt tgaaaccgtt gaaactgctc attttcatta
    14701 ccgcctttca gtattatgtc agacttatca tacttattgt acaccaatat cgtatagaaa
    14761 acgtctgagt atattatata tcatttctta tgaaattgca accccttgaa agccctgaaa
    14821 tattgtacaa aaatcgttaa aacagggctt ttttgtgttg ctttttttga agaatagtga
    14881 tataattgaa ttaagtattt gtaaagctgt ttttgtgttt ttggtttagc tgttttattt
    14941 tatgccgtta caatactcaa tatgtatatc ttttgaagtt tatacagaat aaattgtata
    15001 ttgtctaaaa atatacatta aaaagcctta aaatgtgtat aaatataaaa tttttatgca
    15061 aaagcacttg acaatgctgt ataaaggtgt ataataattt tatcggcatg agagccgtga
    15121 aaatattata caaacatact tactacatac aatggaggaa ttttgttatg gcaaaagaga
    15181 tcaaaaaagt tgtccttgct tattccggcg gacttgatac atctatcatc attccgtggc
    15241 tcaaggaaaa ctacaacaac tgcgaagtaa tcgctgtttc aggaaacgtt ggtcagggca
    15301 cagagcttga cggtcttgag gaaaaggcta tcaagacagg cgcttctaag ctctatatcg
    15361 aggaccttac agaagagttc atcgaggatt atgtatatcc aacagtacag gctggcgctg
    15421 tttatgaaaa ccagtacctc ctcggcactt catttgctcg tccaatcatt gcaaagagaa
    15481 tagctgagat tgctatcaaa gagggtgctg acgctatatg ccacggctgc acaggtaagg
    15541 gcaacgacca ggttagattt gagcttgcta tcaaggcttt cgctcctgat atgcagataa
    15601 tcgctccttg gagaatctgg gatcttaagt caagagagca ggagatcgag tatgctgagg
    15661 cacacaacat tcctctgaag ataaacagag aaacaaacta ctcaaaggac aagaaccttt
    15721 ggcacctttc acacgaaggt ctggatcttg aagatcctgc aaacgagcca cagtatgaga
    15781 agccgggctt ccttgaaatg ggcgtttcac ctatcatggc tcctgacaag ccaacttatg
    15841 taactatcca ctttgaaaag ggtatcccaa cagcagttga cggcgttgag atgaacggca
    15901 aggagcttgt tacaaagctt aacgagcttg gcggtgcaaa cggtatcggt cttgctgacc
    15961 ttgttgagaa cagacttgtt ggtatgaagt caagaggcgt ttatgaaaca ccgggcggag
    16021 ctatccttta caaggctcat gaagttcttg aaactatcac acttgacaag gaaactgcaa
    16081 gagttaagca gtatctttca atcaagtttg ctgacattgt ttacaatggt cagtggttca
    16141 ctcctcttcg tgaggctatg agcgcatttg ttacagaaac acagaagact gttacaggtg
    16201 acgttaagct gaagctttac aagggcaaca tgataaacgc aggtgtaaca tctccataca
    16261 cactttatga tgaagaagtt gctacatttg atgaggacaa tgtttacaat caggctgatt
    16321 cagcaggatt catcaacctc ttcggtctgc ctatcaaggt aagagctaag ctcgagcaga
    16381 agagaaacgc taagaaggac taatagcgtc gctctggtca acagagcata aaggtgtata
    16441 accttaaaaa gtttgtataa tatttttcac ggaggttttg atatgtcaga taacaacagc
    16501 atttttgagc ctgcaataga caagaaggtc gagatctctt atgacagcct gccgaactcc
    16561 tcttttgagg acgtaaacct cggcggagca aagttctcga acgtaaatct taaggaagcc
    16621 gttttcgatg acgttaatat ggacgagaca catttcaaca acgtcagcat gaaagaaagc
    16681 gactttgcag atatgtatat ggtaaacaca aagttcttgg gcgtaaatct cagcaactcg
    16741 agatttggct gctctatcat gaacaatgtt gagtttaaaa atcctgatct taagaactca
    16801 cagttcattc actgtgacct tacaaacgtt gagataaacg attgcaagat agacggtctg
    16861 aagatcaacg gcgtggatat ccagaagctg cttgaacagc acgaaaagga aatttgatcc
    16921 gcacttattg aaatgtatcg gggcaggatg atcctttggg gtcattttgc ccatacattt
    16981 tttatatttg ataacttgaa ataaacataa aggacggttt taaaatggca ggaaaaatgt
    17041 gggcaggaag atttacaaag gaagttgacg aaagagtcaa tgatttcaat tcatctattt
    17101 cctttgacgc aagaatgtac cgctatgata tagagggttc tatggcgcac gccactatgc
    17161 ttggcgagtg cggtataatc gaaaagcacg aaagcgaaaa aatagtccag ggacttaagg
    17221 gtattcttgc ggatatcgac gagggcaaat tacagttcga ccctactgca gaggacgttc
    17281 atatgtttgt tgaggctgag cttaccaaaa ggcttggcga cacaggcaaa agacttcaca
    17341 cggcaagatc aagaaacgat caggtggcac ttgacgtgag aatgatactc agagccgaaa
    17401 caaaagagat aatcgagctt gtgaaaaagc ttgaacacac tatccttgac attgcagaga
    17461 aaaatctcac aactgttatg ccgggatata ctcatcttca gagggcacag cctatcacat
    17521 ttgctcacca ccttatggcg tatgcaaata tgcttctgag agatatttca agacttgagg
    17581 acagctacaa gagaacgaac atcatgcctt tgggaagcgg tgctcttgca tctacaactt
    17641 accctataaa cagacagaga gtttgcgacc tgcttggctt tgacgagata acacagaact
    17701 cacttgacgg cgtttcagac agagatttct gcatcgagct ttgctcagct atctcactgc
    17761 ttatgatgca cctttcaaga ttttctgagg agataatact ttggtgctca tgggagttca
    17821 aattcatcga gcttgacgac gcttatgcaa caggctcgtc tatcatgccg cagaagaaaa
    17881 accctgatgt tactgagctt ataagaggaa agactggcag agtttacggc gaccttaaca
    17941 cactgctcgt tatgatgaag ggtctgccac ttgcatacaa caaggatatg caggaggaca
    18001 aggaagctat ctttgacgct atcgacactg ttaagctttg tcttaaaacg ttcgatccga
    18061 tgctcgccac aatgagagtt atccctgaga acatgagaaa cgccgcagcc aagggcttta
    18121 tcaacgctac tgactgcgct gactatcttg ttaagaaggg tatgcctttc agggacgctt
    18181 acaagataac gggcactctc gttcacacct gcattgaaaa gggctgtaca cttgaaactc
    18241 tcccattgga ggagtacaag aagctcactg acaactttga cgaggacgtt tatgacacta
    18301 tcagccttga cacctgcgta atgcagagaa aagcggcagg cggtcctgcg cctgagtctg
    18361 taaaagctca gatagaatat gtcagaaata agctttgata aacaccgctc ccctgttaag
    18421 tcggcactgc tatatcttgt gctgtgggag cttgcgacag ttataatgtg gctttttacg
    18481 gctaagcttt ttgtgatata tccgctgttt gcggtggggt ttacggcggt gtaccctgtg
    18541 tgtacatggt gggcttgcta ccgacacgct aagaactacg gcttgaaatg gtatgttgcg
    18601 cctgtgatga tagcggtttc ggtgatagaa tatatcttcg tggaggaagc aaggtcggta
    18661 gtgccgaact ttatcgtact cactgtgttg accgcaggct ttgccgcagg tattggaaac
    18721 tgttttgcgg ataaggacgc aataaacgcc gcaaaggaag ataaaaagcg taaaaaactg
    18781 aaaaaagaac ctgaatacaa gaacattttg gacgataatt aaaagtatgc agacggctaa
    18841 agagctgtca gccaaacggg cgaacactgt tcgcccctac aaaagtttgc atttgcatga
    18901 gcatacagat tattgacaga caaaatcata aagttttata aaaggatatg aagaaaatga
    18961 aaacaagagt ttttattgac ggcaaagaag gaacaacggg acttcagata tatgagcgtt
    19021 ttcagaacag aaatgacgtt gagataatgc tcattgacga ggaaaagcgc aaggacgtga
    19081 acgagagagc aaagatgata aactcatcgg atatcacttt tctctgcctg cctgacgcag
    19141 cggcaataga ggcggcttca ctttgcacta accctgatgt caggataatc gacgcttcta
    19201 ccgctcacag aacaaaccct gcttgggatt acggcttccc tgagctttct gaaaagcata
    19261 gagagaatat ccgcaattca aaaagagttg caaacccagg ctgttatgca agcggcttta
    19321 taagccttgt ttatcctctc gtaaaggcag gagtgcttcc tgaggattac cctgttacct
    19381 gccatgcagt ttcaggctac agcggtggcg gaaagaaaat gatagccgct atggagagcg
    19441 aaaataaaac aaaggaaatg tattctccac gtcagtatgc acttgggcag aagcacaagc
    19501 atatccctga aatgcaggcg gtgtgcggac ttaaatataa gcctatgttc aatccgatag
    19561 tggacgacta ttataatggt atggttgttt ctgtgccgct ggtgacaaga tgtttgacaa
    19621 agaagtatac acctgagaat atccatgaaa taatggcgga gcattatctt ggtcagcatt
    19681 tcgttaaggt aatggagctt ggcggaacag aaacgcttcc tgacggattt atcgcagcga
    19741 atacattggc aggaacgaat aatatgcaga tattcgtctg cggaaatgag gatcagatat
    19801 tgcttatgtc gagatttgat aaccttggaa aaggcgcaag cggcgcagca atacagaata
    19861 tgaatataat gttggggata aacgaagcaa cagggctgga ataacaaaaa agcaaaaaac
    19921 aaaccaaaag caccgcacaa aaaatcgccg gtgcagggcg gcgttagccc tgccgaggga
    19981 cgggggcgag cagccccagc gggggcaaaa taaagccaaa agcaaaatca aacccaacag
    20041 ggggcagagc ccccaccaca aaagtacggt ctaaaaaagc ggcggacaaa aaaataaaac
    20101 aaaccgaagt aaaatttctc aaccaagcct aaaaaccaac aaaactccgc cgcttttgtc
    20161 atttgaacag cgaagcgtct tcaaatgact cgccaacggc gacaataagc tcccccacaa
    20221 aggagagcaa ttcccgacca atgagtgcga atataaaagt gcggaaaaaa taagcgggcg
    20281 aacgctgttc gcccctacag aaataattaa tagaaaaatc tgtcctgatc taattagtgt
    20341 gaaaattttg taaggagttt tgaatatgaa agagatatca aagaaaaatt tcgacggcta
    20401 taagtatgta gacggcggcg tgtgtgctgc aaagggcttt aaggctaacg gtctttattg
    20461 ctgcataaag gaaaacccta caaaaaaacc tgacctctgc cttgtggagt ctgacgttat
    20521 gtgttcagcc gccggcgttt ttactcagaa taaggtcaag ggtgctcctg ttactgtttc
    20581 tcagaaaaat cttgctaaaa caggcggcaa ggctaagggc tttatcctca actccaaaaa
    20641 cgctaacacc tgcaacgctg acggtgagga aaaggcttac aaaatgtgcg agatgaccgc
    20701 cgctaaaatg ggcgttaagc ctgaggaaat tcttatcgct cagacaggcg tcataggtca
    20761 gatacttcct cttgaaccta tcgaagctaa gatagacgag ctttatgagg ggctttccta
    20821 tgagggcaac gaaaaagccg ctattgctat catgactact gatacagtga aaaaggaagt
    20881 ggctgttgag tttgagcttg acggcaagat ctgccacgtt ggcggcatgg gcaagggcag
    20941 cggtatgata caccctaaca tggcgactac gctcaatgtt atcactacag actgcgctgt
    21001 atcttccgaa atgctcaaaa aggctcttac tgagatagtg aagataacat ataactgcct
    21061 gtctgttgac ggtgaccagt caacgaatga tacctgtgct gttatggcta acggacttgc
    21121 aggaaaccct gagataacag ccgagggtga tagctacgag gtgttcaaaa aggctcttta
    21181 tatcgttatg atgaacatta caaaaatgct cgcaaaggac ggcgagggcg caactaagct
    21241 tcttgaatgt actgttaccg gtgcaaaaga ccttgacacc gctataatcg ttgcaaagag
    21301 cgttatatgc tcgccgcttt tcaagtgtgc aatgttcggt gctgacgcta actggggcag
    21361 gatactctgc gctgttggat acgctgaagc tgattttgac ataaacaagg tggacgtttc
    21421 actgtcatca aaggcaggag agatacacgt ttgccacaac ggcgcaggca tagagttctc
    21481 tgaggaagtg gctaagaccg ttcttcttga ggacgagatc acaataagcg tttctatcgg
    21541 cgacggcaac ggcactgcca ctgcatgggg ctgtgacctt acatatgatt atgttaagat
    21601 aaacggcgat tacagaagct aagacataaa atcagtatag gaaagaagaa tgagaaatgg
    21661 acataagcaa taaaatcaga agtcagatac ttattgacgc tctgccacac attcagaaat
    21721 accacgacaa gatagtcgtt gtaaaatacg gtggcaacgc tatgacaaac gctgagctta
    21781 aagaagctgt tatgagcgat atcgtgcttc taagtgaggt aggcataaag gtagttcttg
    21841 ttcatggcgg tggacctgag ataaatgcaa tgctcaaaag agtcggcatt gaaagcaagt
    21901 tcataaacgg tctgagatac actgacgctg aaactataga tatcgttaaa atggtgcttt
    21961 gcggcaaggt aaacaaggag cttgtttgcg ctttgcagct tcatggcgga aaggctctcg
    22021 gtctttgcgg ctgtgacggt cagatgatac ttgcgcagaa gcttgacagc gaggtagacc
    22081 ttggttttgt gggcgacatc aaaaaggtaa cgacaaagcc tatagttgac gcacttaaca
    22141 acggttatgt tcctataatc tccacagttg gcgttggcga ggacggacag tcttacaata
    22201 tcaatgctga cactgcggcg gcgagaattg cttcttgtct gagagcggaa aagcttatcc
    22261 ttatgacgga tatcgttggt ctgctggagg acaaggacga tgattccaca cttatcccac
    22321 aggtaaacgt cagcgatgtg ccttatctca aaaagaaagg catcataagc ggcggaatga
    22381 taccaaagat agactgctgt gttgaagccg taagacgtgg cgttaaaaag accactatca
    22441 ttgacggaag agtaccgcac tcgatactta tcgagctgct ctcaaacgag ggtatcggta
    22501 cacagtttat ataaaatatc aatacatatt gaaaagagtg ataaaaatga acaccataga
    22561 acagttcaac gaacacgtta tgcagaccta tggcagaata ggtcttgtta tggaaagcgg
    22621 tcatggtcag acggctgttg gcgaggacgg aaaagagtat atcgacttcg gctccggtat
    22681 cggcacaaac agccttggct actgcgacga ggattgggtg aacgctatct gcgagcaggc
    22741 acacaagata cagcacactt caaactacta ttacacaaag gtacaggctg actttgcaaa
    22801 aagactttgc gaggcaacag gctatgagaa aatgttcttc ggcaactcag gtgcagaggc
    22861 taacgagtgc gctatcaagc ttgcaagaaa atacagcttt gacaaatacg gcaaggacgc
    22921 tgagagaaat atcatcatca cccttgtaaa cagcttccac ggcagaacgc tttgcactct
    22981 ttctgcaaca ggacaggagg ttttccacaa ctacttcttc ccgttcgttg gtggatttga
    23041 aaatgttatc gctaatgata ttgacgatct tatggacaag ctcaacaagc ttgacaacaa
    23101 ggtctgcgct gtaatgttcg agtttgttca gggcgagggc ggagttatac ctcttcagca
    23161 gagctttgtg aacgctatca tgcttgagtg tgcaaagagg gatatcctca ctatcgcaga
    23221 cgaggtacag acaggcgttg gcagaaccgg caagctcctc acatctgagc tttacaacgt
    23281 aaagcctgac ataaccacac ttgcaaaggg tcttgcaggc ggtgttccaa taggcgtgtg
    23341 ccttgctaac aagaagtgca gtgaggtact tggcaagggt acacacggct caacgtttgg
    23401 tggaaatcct atttcctgtg caggcggaaa cgcagttctt gacaaggtga caaagcctga
    23461 atttcttgag gaagttcgca aaaagggcga gtatttcaga gaaaagcttg tccagataga
    23521 cgaagttgaa ggcgttgacg gacttggtct tatgataggc gtaaggctca aaataaagaa
    23581 agccgctgat gtgcttaaca aatgctttga aaacggactt cttattctca cagcaaagga
    23641 taagctgaga tttttgccac cgctgaatat ctcatatgag cagatagaca agggacttgc
    23701 tattctgaaa aaagttcttg acgagaaata acaaagtgtg taggtgcgaa cagtgttcgc
    23761 ccgtttgata tgcaatacca aataaaaatc aaattacgga ggaaatatta tgaaacattt
    23821 acttaaaatg ctcgaccttt ccaaggagga aattctcgac attctcaatc ttgcagacca
    23881 gctcaaatac gaaaacaaaa atggtatcga gcaccacatt cttaaaggca aaactctcgg
    23941 aatgatattc cagaagtcct ctacccgtac aagagtttct tttgaaacag gtatgtatca
    24001 gcttggcggt caggcacttt tcctttcaaa ccgtgacctc cagataggca gaggcgagcc
    24061 tgttcaggac actgcccgtg ttctttcacg ttaccttgac ggcatcatga tccgtacttt
    24121 tgagcagaaa gaggttgagg atcttgctaa atacggctct atccctgtaa taaacggtct
    24181 gacagacttc tgccaccctt gtcaggtgct tgctgacctt atgactatca gagagttcaa
    24241 aggacgcttc gagggtctga aaatgtgcta cataggcgac ggcaacaaca tggcaaactc
    24301 tcttatcgtt ggcggactta aagtaggcat ggaagtttct atcgcttgcc ctaaggatta
    24361 tcagcctgct gctgaggttc ttgaatttgc caagggctac ggcgacaagt tctcaatgac
    24421 agacgttcct cttgaggcag caaaggacgc tgacgttctg ttcacagacg tttggacttc
    24481 aatgggcgag gaagctgaga cagagaagag aaaaatcgca ttcaagggct atcagataaa
    24541 tgacgatatc atggctgttg caaaggctga cgcaatggtt cagcactgtc tgcctgctca
    24601 ccgtgaagag gagataaccg aaaaggtatt cgaggctcac gcaaatgaga tattcgagga
    24661 agctgaaaac agacttcatg cacagaaagc cgttatggtc aaggtaatgg gtccgcagga
    24721 ataattcgca tatgacaaaa gccgtccacg ctggtgggcg gcttttttgt gttgaacaca
    24781 tcaaaaaggt ctttgatgtg acgggataaa aagatccgca gtcctcaaag aactgcggtc
    24841 atagttaaat aacattttca tttacgatat ggtagctttg cagaatttct gggtcaaact
    24901 gaatgagtga gatcattctg cgtgcaggac cgataggctg gttcgtacct gtttcccacg
    24961 cctcaacagt ttttgtcgaa actccgatca ctgcggcaaa taccgcctgc gtaaatccca
    25021 gcttttttcg gatcgctttt atatcttcag cagatatatc aggcaaaggc tctattctaa
    25081 gagttgtttt ttagcagaaa ttttaccttt ttcgtagtca actgcctcgt taagtccagt
    25141 aattatgctg tcaaacacat tcatattaaa gctggtctcc ttcgtattta ttattgaagt
    25201 tgtaatatac tttctcattt ggaagcactg ccttaatcgg ataaactgac atcccaaaag
    25261 aatctgtaaa aataacatct tctccaatat gtttttcaag aaactcatca gaaaaatcag
    25321 atattaaacc aatgctgata gctccattcc ttgtaaaatt caatnnnnnn nnnnctgaag
    25381 tgtgaaaaaa attcaacaac attttttgaa tatctcgttc gatttgaatc tgattatcaa
    25441 gataatatct gattgcttct tcttgttcaa atgaaattac agcatctttt tgatttgagt
    25501 atatacatag atacaggtca aaagttttat cccataatgt aatgataaag ctcttctttg
    25561 tgtaccaatc tgttttttta atcaaagttc ctaatacagg atgctcaatt gtagttggtt
    25621 tatttttttt ctttgtcttt ccaaaaaaca taatctcgcc tttaaaattt gtatggctta
    25681 gcccattact acctcaacat ttactgttgc accattttca gctacaggga gaacattgcc
    25741 ctctactgcc ttgccgtcaa cagttacgct ctttatgccc ttgcaaacgt ggtcagggtt
    25801 cttaacagtg atgttgtatg ttgcacctct gtactgacgt gttgctgtca tgccgtccca
    25861 ctcgtgaggg atagatggat ctatcttcaa gccgtcaaag tcagcctgta ctccgagaat
    25921 gtactgtgag atagctacca tgttccatgc ggctgtacct gtgagccagc tattcttgcc
    25981 ctcgccgaat ctgcttgcgt ccttaccagc tatcatctga ccgtatacat aaggctctgt
    26041 cttatgaaga tcagatatct cctcgttgaa tgcaggagct atcttgctgt aatactcaaa
    26101 tgccttgtcg cctctgcctg catatgcctc tgcgcagatt atccaagcat tgttatgtgt
    26161 gaagatacca gcattctcct tatatccgcc cggatatgtg gatatctcac catactggat
    26221 ataatacttt gaatatgcag ggttgttaag aacaagacca tactttgtat tgaggtactt
    26281 gtcgatagaa tcaagtgtct tgatgtcagc gccctcgtcc ttgcctattt cagacataac
    26341 tgcaaagccc tgtggctcga tgaatatctt gccctcttca cattccttag aacccatttt
    26401 tgcgcctgtg gaatcgtaag ctctcaggaa ccaatcgccg tcaaatgcag attccataac
    26461 gttcttcttc atcttgtcta tctcagcctg tgctgcagca gcttcatcgt ccttgccaag
    26521 atgcttgagg atagacacat agttagggcc tgtgtatgta aagagtgttg ctaccattac
    26581 agactcagct atctttgagt agccgccctc ggctgcaaac ttagggtttg tataagtctg
    26641 gaagctctcg cccggagtat cagaatagca tgaaaggttg atacaatcgt tccagtcagc
    26701 tctcattgca agtggcagac cgtgaggacc gaggttattt acaatgtgat agaatgatac
    26761 cttaaggtgg tcgagcattg gcttagcctt tgactcgtca ttatcgtatg gtaccatttc
    26821 gtcaaggata gaccagtcgc ctgtttcctt gatgtatgcg gaaactgaaa ggatcatcca
    26881 cagtgggtcg tctgagaagt cgccgccgat atcggagttg cccttctttg taagaggctg
    26941 atactgatga tagcagccgc cgtcaggaag ctgtgtagaa gcgatgtcga tgatacgctg
    27001 tcttgcacgg tccggtatct gatgaacgaa gccgaggata tcctggtttg aatctctgaa
    27061 gcccattcct ctgccgatac cgctctcgaa atatgatgcg gaacgtgaaa ggttgaatgt
    27121 taccatgcac tgatactggt tccagatatt taccattctg tttaccttgt catcaggtgt
    27181 gtcaacattg ataacgctga gaagtctgtc ccaagttgcc ttgagttctt caagacctgc
    27241 ggctaccttt tcaggagtgt tatactgctc gatcattgcg agagcctttt ccttattgat
    27301 agtaacgccg tcagcctcga acttcttgtc aacaggcatt tcaacatagc cgaggataaa
    27361 tacaagagtc tttgactcgc ccggagcaag tgttatcttc ttgtagtgtg aagcgatagg
    27421 tgaccagccg tctgcaaatg agttgttagc cttgcctgct tcaacagcct gtgggttatg
    27481 gaagccgtta taaagaccga taaatgaatc tctgtctgta tcatagccgt ctatctcatc
    27541 atttactgta tagaatgcaa agtgatctct tctgtctctg tattctgttt tgtgatagat
    27601 agttgaacct actacctcaa cacggcctgt gctgaagttt ctctggaagt ttgtgcagtc
    27661 gtcctgagcg tcccaaagac accactctgc aaatgagaag aatgagaatg ttttttcttc
    27721 attgctctcg tttgtgagaa ctacctgctg aacttcgccg ttatagttct gaggaacaaa
    27781 gaatgtagcc tcagccttaa gtccgttctt cttacctgtg atgatagtat agcccatacc
    27841 gtgacggcac tcataaaaat caagctctgt ctttaccggt gaccagcccg gattccagat
    27901 agtttcgccg tctttgatat agaaatatct tccgcccatg tcgataggca cgttgttgta
    27961 acggtaacga gttattcttc tcagtcttgc gtccttatag aaggaatagc ctcctgctgt
    28021 gttggagata agtgagaaga attcctgtgt gccaagatag tttatccatg gatatggcgt
    28081 tcttggattg ctgatgacat attccttttt cacgtcatca aaatgaccaa atttcatcaa
    28141 taatttccac ctttcgtaat ttatctattg ttaattaatt tcttaattat aaccattgtg
    28201 aagtgtatag ccgtcacaaa gtgatcaagc caacgggcga acgctgttcg cccctacatt
    28261 tacagagctg ttacataact ctcattctgc ataatgatag tttttctatt attcagtaaa
    28321 gaatctctgc cagcacttga atgtgtgacc ctttgttatc tcctcatatc cgctcacttc
    28381 gttaggcagg ctgagattag gagaatttat catagctgtc attggttcag ggcagaaata
    28441 tcctttattg cccttatcgt tccacatatt ccagaactta aattcttttg acacctcgtt
    28501 gcaaacctta tgaccattgt cagtatccac aacataagag ccatggaagt ccttaccgtc
    28561 aagcttgttc atgcctgcgg tgtacatatc gtttgagata ttatgcaggg taggcatttt
    28621 tgtaccctct ttatattcaa ggtctttctt tttaagaggc aaaagctttt ctgttggcag
    28681 acatctcttg tcaagctcgc acttttcgat tatcggcacg cacattctca ttgattcctc
    28741 cctgccaccg tcgcacattg gcgcattaag gcaggtatga gtacatatgg aaacaggcag
    28801 agccttgtcg ctgttgtttg tcaggtatat ctcctgcaaa agaccgtttt cagaaagcgt
    28861 aaacgtatag gatattttaa aatccaccgg aaaataagaa tacatctcgt cattcttgtc
    28921 aaaggtataa gaagtaacaa gcacaacttt tttatcctgt gttgaataac actctatttc
    28981 gtgttctctc ttatgcaccc agccatggat aaaattatca agctttttct catttacagg
    29041 catttgatac actgcgtcag aggtctttat aatgccctta tcaaagcgat taggcaagaa
    29101 aagagtaggc agtccccata tctctctcgc cttatttata gtcttgagag tgcagtcatc
    29161 acgatatctg aacacctcta tgccgttttc ctcgtcacga agcctcagca cagaagcacc
    29221 cagttttggt gcgataacgc aggaatactt tcccgactgc attctgacac attccgtatc
    29281 tttaaacatg aatttaaggt ctatattatt caaaaaagta acgcccccat agtgcaaagc
    29341 gatgatcttc ctcccgcttt accatacatt tttacttaaa cgattaacat acaatcatta
    29401 taacatagcc cgtcgctttt gtctagtaca aaacgcagat ttttatatag aattttagct
    29461 gtgtttttta gtagtttttt accaaacgtt taatgaataa tccaaaaaaa tatgacaata
    29521 ctacaattaa cagcccgctg acagacggac tgctctatat tttaaacaat caaaggagga
    29581 gtttctcatg atagatagga tcgcactgct gcttctcata ataggcggca taaactgggg
    29641 gcttgtgggc attttccagt tcgaccttgt ggcatttctt ttcggaggcg ctgcagcgat
    29701 actaagcagg atcatctacg ttttggtagc tatttcggct atctggtgca tttcactgtt
    29761 tttcaaagac aacagacttg tcaccgagca gtagtcccct gcaggtcatg acggcaggag
    29821 agggagcttg tttcagaggg cagcatttta tcacgggttt gcttgctgca tattctaaaa
    29881 tcggctctct ttcctgctgt catgacctta tttttttgtt ttaaacatta aaatttgaac
    29941 atcgcagaca ctaaaaatgg ataagatatt ttactttttt aagtcgaatt aagttcagac
    30001 ctcacgcaga tgtcattgtt agcataatgt caacgaaata tttgtgcaaa atcccgaatt
    30061 ttcagaaaat taacaagttc tttgcaactt tttcttgact atcgctacaa aagaggttat
    30121 aaattaattg atgttaagga tattgaacat aatttgtttg atttaacatc cgttttgttg
    30181 tctcctttct tttgttctac acatagtttt ttttatttta ttttctgctg agcaccgttg
    30241 ctcagcattt tttttgccca ttttatgcac acgttgaaaa attcatattt tagagttata
    30301 atgttacctt atggactaat aaaacaatat gtcagttctt atctgtcaaa acgttttcgt
    30361 cgtgaaagaa aggagtaaat agaatatgag atatcttata gttgttgata tgcagaatga
    30421 ttttgtcacg ggcagtcttg gttcaaaaat ggcagaaatg attgttcctg ctgtctgtga
    30481 aaaaatacgc aattacaagg ctcagggcag cactgtattc atgacccttg acacgcattt
    30541 caaggattat cttgaaacgc ctgagggaaa aaagctccct gtggagcatt gcataaaagg
    30601 caccgagggc tggcagcctg agactcaggt agctgaggct atcggtggaa gcgcagaggt
    30661 ctatgaaaaa agcacattcg gctcgactga gcttgcacag caccttgctg aaataacaaa
    30721 ggcaggggac gagatagagc tttgcggact ttgcacagac atttgcgtgg tatcaaacgc
    30781 tttgctaata aaagcttttt gtcctgaatg cgaggtcata gttgacgcaa acgcctgcgc
    30841 aggagtaacg gaagaaaacc acaaggcggc acttgttaca atggcgtcat gtcagtgcag
    30901 gatagataat ttttgatttg agggggaaca ccgcttcggc ggaatgattt atgagtggtt
    30961 tcaaaaaagg tcttaaagac ggcataccta ttgctttggg gtatctttcg gtatctttta
    31021 cattcggact tcaaggcgta aaaggtggtc tgagctggtg ggaaactgtg cttatctcta
    31081 tttgcaacgt gacctcggca ggtcagttcg cagggctgga cattatgctg tcggggggcg
    31141 gtttctggat agaaatggct tgtgctcagt ttataatcaa cctgagatat gcccttatgt
    31201 caatatctct ttcacagaaa gctgacaagt cactgtcggg aatacaccgc tggctggtag
    31261 gcttcggagt gacagacgag atatttgtca tggcaatggg gaatataggt gaagtttcaa
    31321 ggcagtatat gtatgggctt atcgccttac ctgtagcagg ctggtcgctg gggactctta
    31381 taggtgcggc ggcaggaaaa atacttcctg agatagtcat aagcgcactt agtattgcaa
    31441 tatacggaat gttccttgca ataataatac cgcctgcaaa gaaaaacaag ggcattgcaa
    31501 tggcggcgat atttgcggct cttcttagct gttgtttcaa gtggctgcct attcttaata
    31561 aggtatccgg aggattttcc ataataatct gcaccgttct ttccgcaggg ataatggcgg
    31621 ctctcatgcc tgtgaaaaat gaggaggacg gtcaatgctg agtataagag atttcttcat
    31681 ttatcttttc attatggcag gagtgaccta tcttattcgt gccctgccgt tggtaatttt
    31741 caagggcaag ataacaaaca gatttgtgca aagctttctt tactatgtgc catacgctgt
    31801 gcttggagca atgactttcc cgtccatact attctcaacg gaaaaccttg cagcttccat
    31861 agcaggactt ataacagcct gcgtgcttgc attcaaggaa aaaagcctta ttatagttgc
    31921 ggcatttgcc tgccttgcaa gcttctgcgt gatacttatc tgtcagctta tttaaagttt
    31981 tgtgcagagg ggtgacttga ggtcgcccgc tataaatttt ggatatttga caagctcaga
    32041 cctttctgct atatgcggag aggtctttat tattactatt tttatatagc atatgaaatg
    32101 tcgtaaatgg agtataaaat tttcggctag tttcatattc ttttcttgca aaaaattctg
    32161 ggctgtgata taatcttatt gtgtgaaaac gtttactgtt tttgatctat gacaaaatat
    32221 atggaggtat tgcaatgtca gacacaatga acatttctcc tgagctcttg gagaaaatga
    32281 acaggtattg ggaagctgcc aattatcttt cggcaggtca gctctatctg cttgacaatc
    32341 cgcttctccg tgagccgctc acaatggatc acgtcaagaa aaagatagtt ggtcactggg
    32401 gtactgtacc tggtcagaac tttatctatg ttcaccttaa cagagtcatc aagaaatatg
    32461 accttgacat gatctatatt tcaggccctg gtcacggagg aaacttcatg gtagccaaca
    32521 cctatcttga aggctcttat tctgaggtat atcctaacat ttcaagagat gttgagggta
    32581 tgcagaagct gttcaagcag ttctctttcc caggaggaat ctcatcacac gttgcgcctg
    32641 aaaccccagg ttctataaac gagggcggtg agcttggtta ttcacttgca cactcattcg
    32701 gtgctgtatt tgacaaccct gaccttatca caacttgcgt tgtcggcgac ggtgaggctg
    32761 aaaccggtcc tcttgcaaca gcatggcact caaacaagtt cctgaatcct aagactgacg
    32821 gtgctgtact tcctatactt caccttaacg gctacaagat atcaaatcca actgtattct
    32881 caagaatttc tcatgaagag ataaagagct tctttgaggg ttgcggctgg aagccatact
    32941 ttgtcgaggg cgacgattct atgacaatgc acaagctcat ggctgaaact cttgacactg
    33001 ttatcgagga aataaaggct atccagaaga acgcaagaga aaacggcgtt actgaaagac
    33061 caaagtggcc tatgataata ttccgttcac caaagggctg gacaggtccg aaggttgtag
    33121 acggcaagca gatagagggt acattcagag cgcatcaggt accaatgaca atggaagctc
    33181 ctgagcattt gaaaatgctt gaggattggc tcaagagcta tcaccctgag aagcttttca
    33241 cagaagaggg cagacttatc cctgagcttg aggagcttgc tcctacaggc gacagaagaa
    33301 tgggtgcaaa cccacacgcc aacggcggac ttcttctgag agatctcaga cttcctgatt
    33361 tcaggaaata cggcattgat gtgcctgctc ctggtgcagt tgaagcacag gatatgatag
    33421 agcttggcgg atttgtaaga gatatattca agcttaatga agaaagcaga aacttccgta
    33481 tcttcggacc tgacgagaca atgtcaaaca gacttggcaa ggtctttgag gcaacaaaca
    33541 gagattggaa cgctgacaag ctcgacaacg acgagttcct tgcctctgac ggtcgtgtta
    33601 tggactctat gctttctgag catatgtgcg agggttggct ggagggctat ctgctcactg
    33661 gacgtcacgg tttcttcgca agctatgagg ctttcatcag aatagttgac tcaatgttct
    33721 cacagcacgc taagtggctc aaagttactt cacagctccc atggagacag aagatagctt
    33781 cacttaacta catactttct tccaacgtat ggcagcagga tcacaacgga tttacacatc
    33841 aggatccagg cttcctcgat cacgttgcaa acaagaaagc agacgttgta agaatgtatc
    33901 tcccaccgga cacaaactgt ctgctgtcat gctttgacca ctgcatacgt tcaaagaact
    33961 atgtaaacgt aatggtaaca tcaaagcacc caagacagca gtggctcaca atggagcagg
    34021 ctgttaagca ctgcacacag ggtatcggta tctgggaatg ggcttcaaac gatcagggtc
    34081 aggagcctga cgttgttctg gcttgctgcg gcgacacacc tacacttgaa gcacttgcgg
    34141 cagtaactat cctgagaaag aatcttccac aggtaaagat aagatttatc aacgttgttg
    34201 acctgttcaa gcttcagcca cagtcaaagc acccacacgg actttccgac gctgactttg
    34261 acgcactgtt cacaaaggac aagcctatcg tattcgcatt ccacggctat cctacactta
    34321 tccacgagct tctctatcat cgtcacaacc gcaaccttta tgtatgcggc tacaacgagg
    34381 agggtacgat cacaactcca ttcgacatga gagttcagaa cgagatagac cgtttccacc
    34441 ttgttatgga agttatcaag agacttccac agctcggaaa cacaggcgct tatctccacc
    34501 agctcatgca ggacaagctt gttgaacaca agcagtatat tgctgagtat ggtcttgata
    34561 tgcctgagat cagagattgg aaatgggata tctgataaaa aagcctttct attaattaaa
    34621 tatacccaca caaaaaggga cttccgttta ttcgggagtt ccttttttgt gcataaaaat
    34681 atcccccgcc ttgcgacgag ggataaaaat actctagcac ataaattact ttctgttgtt
    34741 tgctatcttt gcaagaagat tcagtatttt aagataaaga taaataacgc ctacgataag
    34801 accatagctt gcataccatt cannnnnnnn nnggaagccc ctgttcaact gtcatctgga
    34861 tagtttcaaa atcacacata agaagcgctg cggcgataag aacgccaata gcagcgaaaa
    34921 gtatgccgat agggccgttg tttattgcaa cgattgaagt gtagatagct gaatgaggtg
    34981 cgagccatgc aagaagcatg aagagcacgc cgcctatgat agaaacccaa aggcaggtga
    35041 taagagcagt tttcattctg cttcccacac gaacgccctt tgaatagata actgcaagca
    35101 ctgcaactgt gagaagcgtc agtgtaacag cttcaactat tatgcctttc cactgcattg
    35161 cgtagatcat tgacataaag gtgagggcat atcccatgcc tgcggaatag atagagcctg
    35221 tgaccgccgt tgttttcgga gcgaatgaag ccacaagtcc tgctattgct gttgcgataa
    35281 gagcgccgac aaagatagta tactcagtgg aaaaagcctg cgcaccgccg ccgaagaagt
    35341 tgtgaatgta aaaatatgct cccatgcctg cggcgaaaac tgcaacaaaa tacaaggtct
    35401 tcattgcaat gcccttatag cttgccgcct ccatgccgta agtggactgt gcaaaagagc
    35461 gcagcttttt cactcgtgga tttgaatagc tttcagaaaa attgttataa tcgttcatga
    35521 atttgcctcc tttatttttt tcgccaatat ttattttcat ggcttatata ctattataca
    35581 tattttctta aaaaagtcaa tatgattttt caggcaacaa cgcaaggttc ttttttggga
    35641 cttgtccgca tatgatgtaa gcaaaggcaa aagcactgaa aaatcttgtg cagtcaaaat
    35701 gtttcgcaca atgttatcaa ggctatgcca aaaaatcgga aaggaagata cattgtgctg
    35761 gatatacttg aaaagaagct tacggaaaac cagaccacaa gaaaggggct aacaactgcg
    35821 gaagctgaaa agcggctcag tacggacgga gagaaccgcc ttgccaaaaa gaaaaagacc
    35881 tctgcggcga aaatttttgc aggacagttt catgacgtta tggtgatgat acttcttgtg
    35941 tcggtggtga tatccgtagc acttgggcaa tatgctgacg ctgtgcctat cgtgcttata
    36001 gttatcatta acgctgcgct gggatttatt caggaatacc gctgtgaaaa aactctggaa
    36061 aagcttgaaa atatgaccgc tcccaccgcc atggtatatc gtgacggcag gcttgtgaaa
    36121 attcctgcgt cggaggtcgt ggcaggagat gtttttactc tcgaagcagg tgacagagtg
    36181 ccttgcgacg ggtacataaa ctcatcaagg gcgttatcat gcgacgaatc tgcactcact
    36241 ggcgaagctg tgcctgcggt aaagaaatgc cgtacatctg agatagattt taccgctttg
    36301 aataaggatt acatggtata catgggaacg gtggtaacaa agggcgttgg cgaggtcacg
    36361 gcggtggcaa ctggtgagcg aagccaaatg ggcagggttt caaccatgct tgatgagata
    36421 aaagagcctg aaactccatt gcaaaagaaa ctcggagagc ttggcagaac tcttgctatc
    36481 atctgccttg cagtgtgcgt ggtagtcttt attgcaggcg tactaagggg cgagcctgtt
    36541 ctcaatatgg caatgacagg aataaccata gccattgccg caatacctga aggcttgcct
    36601 gccactgtga ctatagcgct tgcccttgcg gtcagaaaaa tgctcaagcg tcaagcactt
    36661 gtacacaggc ttcattcagt ggaaacgctc ggctgtgcca ctgttatctg cactgacaag
    36721 acaggcactg tgacaatgaa ccaaatgaca gtcactgaca ttttcacctg caccgagaaa
    36781 agccgtgctt acggcgtaac aaaagaggga gcaagctttg atgacaaagc cctcaaagca
    36841 tgggaaagcc ctgaccttgg caggatactt ttgtgcggcg cagcctgcaa caacgcaagg
    36901 ctctgcccgc ctgaaaagat aaaaaagcgt gacaggggcg gcaggcaaag cgagctttgt
    36961 gcagagggcg accctacaga aacagctata ctcatagcct gcgcaaactc aggtataaac
    37021 gtcagctccc ttggttacag acgaactgac gagcttccat ttgaaagtga aacacgctcc
    37081 atgacggtaa tatgcgctga cgaaaagggg gttacaacgg cattcaggaa aggcgctttt
    37141 gacgttatca taaaagaatg cagccacgtt ttttctgaca gcggcgagct tttgacattt
    37201 ggcggagcaa tgagaaaaca ggctttttac aagtgcgatg aatacgcttc aaaaggtctg
    37261 cgtgtgatag cattttcaca gcaggtggac ggggaatggg cttttcttgg actaatggca
    37321 atgaaagacc ctctcagggc tgaagccgca aaggctgtgg cggaatgtca gcgtgcaggc
    37381 ataaagacag ttatgataac aggcgaccac aagctcaccg ctcaggctat tgcaaaagaa
    37441 gccggcatta tgaaagcagg ctctatcgct ttgacaggcg acgagcttga cagaatggac
    37501 gacaaacagc ttgacgaggt aatagaaaac tgccgtgtgt tcgcaagggt gacacctgaa
    37561 cacaagctca ggatagtaaa ggcattcaaa agccgtgggc acgtttgtgc aatgacaggc
    37621 gacggggtaa acgacgcacc tgctataaaa gaagccgaca taggtgtatc aatgggaata
    37681 tcaggcacag aggtcacaaa gcaggcggct gacgtgatac ttcttgacga taattttgcc
    37741 acccttgtga actccgttga ggagggcagg accatatatc agaacataag aaaattcgtg
    37801 cgctacctta tttcctgcaa cataggcgag gtcataacaa tgctcggtgg aattttaatg
    37861 gggcttccaa tggtactgct tccagcgcag atacttcttg taaaccttgt tacagacagc
    37921 ctgcctgcaa ttgcgctggg gcttgagcct gcggaaagct cggtaatgaa aaagccccct
    37981 agaaaagagg acgacagctt tttcagcggc ggacttatgt ggcatatcgt cataagaggg
    38041 cttcttatcg gtatctgcac ccttgcaagc ttcactgttc tgctcactaa cacccattct
    38101 ctcggcactg caaggacagg tgcgcttata acccttgtgc ttagtcagct tatccatgtg
    38161 tttgaatgta aatcggaaga aaagacgctt ttcactgtgc cttatctttc aaacccattc
    38221 ctgcttttct ctgtattcat atcagcggca gcgctgtttg caggcatatg gctccctgtt
    38281 ctgcaaaagg tatttttcac agccgttccg agccttggag agtttcttgt ggcgacggca
    38341 gcggcggcta tcgtgcctgt tggtgcagga ataataccaa agctttttac gggcaaaaaa
    38401 tctcctgacg taacagtgaa cgccccgcag ggatagcaaa agaccgacag ggcaaagctt
    38461 tgtcggtcta attttttaat ccatattttt tatccagcct tttaaatatc ctgccaaaag
    38521 gttttgataa aaccagcagg aagaacacgt tggaaactgc gtacataagg gtaaaaaaag
    38581 ctgacgagcc aacaagggca aggtatctgc tgagaataaa ggcgttatca gcccacagac
    38641 agctccacaa gtccatgata agagagaacg ccgttcctga gattatgccg aaggtcacaa
    38701 ggagtggctt gctttttata agcttatcag ccaacattcc tgcaaaatat ccggtcagac
    38761 cccaaaccag catttgaaaa ggcgtccacg caccctgtcc gaaatagaag tttgagataa
    38821 gggctgtcat tgcgcctgtc ataaagcccg attctcttcc catataaagg gcggttatga
    38881 taacgattgc ggtacagggc ttaaagcccg gcagaaagct gaaaactatc ctgccgaaaa
    38941 cggacaatgc ggtcatggcg gctattatca caagctgtgt actgtcggag tgctttttct
    39001 caaagtggag aaaatagcct gccacggtaa gaatggcaag tgctgcggtg acgaaagaat
    39061 agaagcgttc tctgaaaaac attataccgc caaagcacac aaggggtatg acaagaagat
    39121 agataagccc tgacacaagt tttttactgc tcatattgac ctcttttcac cattgcgagc
    39181 cgttctctta cggctgttga cgtcaccgca ccactgaaaa agccccttgt gagccttgcg
    39241 gcggcggtgg tgtaaaagcg attttgggag aaaaactcca cggcattatc ctcggcgact
    39301 atctttccgt caaaaagaag tccgcacctg tcgcagtatt gtgcggcaaa ttcaaggtca
    39361 tgagtcacga gaagtatcgt cctgccctcg tcttttaggt ccaaaagaat atcaccaaga
    39421 gcctttttag cgaaactgtc catgcccttt gtacactcgt cgaggactat tattttcggg
    39481 tcagcaagaa gaatttttcc aaaagcacat ttctgtatct cgccgccgct gagatcccaa
    39541 gggtggcggt caaggagatg ttctatgccc atgcgctgtg aaacattttc cactgtgact
    39601 ttgtcaccgc tttgcaacag atcctcacgg acgctttctt ttacaaacat tgtcacaggc
    39661 tcttgtggca aaaatgcaag catttcacgg aaaagcgagc cgtttttata ggctttctgc
    39721 tttttgccaa aaagggatac agcacccata taaggcttgc taagtccgca tatcacacgc
    39781 aggagcgttg attttccacc gccgttcgag cctaaaagcc catagcactc cccttttctg
    39841 acggcaagat cacagccttt gaggatatcg ggggagtttt tttcatatct ctgccacaga
    39901 tttttgcact gcagggcata aggctcagca cgttctgtat cagtgacagc ctgctttgga
    39961 atatccgtca cgccaagctt ttcaagcttc gccctgcctt gtcttacatt gaggggcagg
    40021 tcgtcgcaaa gccttgcgaa cgccttggcg gtttcgggaa gaaagccctc cagagcgttc
    40081 tccacaaaat attttgcagt caaggcaggc gtaccgaaaa actcggtttt gccctttgca
    40141 agatacaaaa ttttgtcgca ggagtcaaaa agctcctcgg ggtcatgctc ggcaatgatg
    40201 acagtcacgc ccatttcttt gttaaggcgg tctaaaatgc caagaagctc ccttgcggca
    40261 acggggtcga gctgtgcggt aggctcatca agaagcagga tatcagggtc tgtggccatg
    40321 acggaacaca gagctacgag ctgtttctga ccgcccgaaa gtgtggagat atccctgtca
    40381 tagagcggct cgataccgaa aaaagccgcc atttcaccaa cacggctttg tatctcacca
    40441 cttttcacac caaagctttc aagcccgaaa gcaagctcag ccgatacttt gtcgcatacc
    40501 gtctggctgt cagggctttg cattacaaag cctatctttt cggcactttc tctatcagaa
    40561 agctcgcttc tgtccttgcc cataagggtg atgtccccag aaagctctcc ccttggggaa
    40621 agctctcttt tcagaagcct gagcaaagta gattttccgc ttcccgaagc accgcagagc
    40681 attatttttt cgcctttttt cactgaaaaa gtgcagtcag aaatggcata gtccgtgctt
    40741 tcgttatatt tgaaagctac attttcgcaa gacaatattt ccattttatg ctctccttta
    40801 cttcaagaat aaatgggaaa acgcatagta atgcgaacat tcccacaggc aaaacgctgt
    40861 tttcaagctt tatcacaggg taaaaagcgg cggacagctc gccccttgcg gaaaatatca
    40921 gcaccaatgc aaagataact acaaaaaccg ccacaaaaat gccgtcccat gctctgaact
    40981 tgaacgaatg tgcaaagctt cgtttctgga taccatagcc acgggctttc attgaagcgg
    41041 ctgtgtccgc tcccctttca agcgtgcctg cacaaagggc tgtgaacact gtgcagtctt
    41101 ttttcaggcg gtcaaaacgg ctgtcggaga tgttcgcacc agagcagttc aaagcgtcca
    41161 aaagctgtct gtgaagcctt ttgagctttg ggataaagcc cattgtcatt gttatcacaa
    41221 gggcggcttt gggcgttttt cggccaaaaa tgtataggag cttttccctt gtaagcacct
    41281 ttccaagagc tttgcaccac attatcacag cggtgagnnn nnnnnnnggt gagggatagt
    41341 ccagcggtca agccataaaa aagggcttcc tttgtgtaag ccttgccgtt tacgaaaaac
    41401 agcggcgtta cgcccttatg tgaaaaaagt ggatttgtga cagtcaaaac ggcggtaaga
    41461 aagatgtaga aagcaaggtc tgatgcggtc tgtctacagc tttgagttat ggcacagaac
    41521 aggaacgctc ccactgccga gcatatacca acggctggtg aaaagcagaa cattgtcacc
    41581 gcaagcacag ccgcaaaata cacaagggcg gtcaaagggt gaaaagctga caacctcatt
    41641 tcaccgcctc ctttgtgtag tcgcaaacgt agtaaaactc cacgctgtca ccgtcattga
    41701 ggacatactg tgaacagccc ttatcggggc tttcgccatt taccgtatag gtccaccctg
    41761 aggacggacc gcaggaaaac tcgtagatat tgtttatgcc acggacatac acagtgttgt
    41821 aaatattatc ctctgagcct tgataatcaa ggggtatctt cccgcacctt gtagcttttt
    41881 ccaacagatc aaaggcggtg tcgccctcac ggaggacata tttttccgca ggcataacaa
    41941 tgccgtctga gggaacatag cactcgtcac ggagattttt gtcaagcata tcgtaattat
    42001 ccaaaatatc agagcaatcc actgtcagag tgacagtgtg agcggcattt gcagtatcgt
    42061 cgctgtgcag gctgtaatac tcgtcaacgg actgaatatc aactcccttt atgaccgctg
    42121 ccgctgctaa aattatgact atgagcagag cgatatcctt tttcactttt ctgctccctc
    42181 ctgaactttg agctttttgg ctttctttgc cgaaactgca aagtttttca ctatatgcat
    42241 ttcctgtggg gaaatctcga gccttgaaaa tctcgccttt tcgtatattt tctcgcactc
    42301 tgcgaagatt tccgccatat cagcgccaaa ggtctttttt atgctctcgg aaagctctct
    42361 tggcgtatgg tctgagaaat tcctgccgca aagctttttt ctgcctgcaa agaacagctt
    42421 ctccaccttt ttgctgtcag gcagagcggc aaactttctt tttcttacca aagctattat
    42481 tgccacaata aggcagagta taagtattac cgcacctata acgatatata ccgcatagct
    42541 tttctccgca ggtttagtct tgttctcctc aaagatctct tcccttggtt tgtcctcgtc
    42601 ctcgggagca agggcttcgc cgccttggtt ggtgggcttt gcttttacag agctgtcgtc
    42661 agtttttatc atgccaacat aatcggggca gacctcgaaa ggctgccagc ctatgccgtc
    42721 tttgtaatac tccgcccaat agtggagctg ttttgaagtg acggtcaggg ctgagttatc
    42781 tgtaaggcct ttggtcatat ctgatgtgat aaaatatcct ctgcaaagcc tggcaggaac
    42841 gccctttgca cggaagatct tcacaacagc ggcagcaaaa tcctcctcgc ccccacggca
    42901 agttacctca agcatatctg caaaagagct ttcgccccca taggtctttg acctgtcaag
    42961 cactgcgtct gacacaaaaa agcttagcat atcagaccgc agtttaccct gagaaccttg
    43021 cagatcaaaa tggtttgcgg caagctgttt gtcctcggct gatatgtcaa ggcaggtgga
    43081 ataaacatat tcacgataac ccttttctgc ggcaagataa tccgcaggct cagaagtgcg
    43141 cagggtatca aatgtgccaa aaggctttgt gagatagcct tccgacaccg caagcctgta
    43201 atagcttttg gtgttctcaa agcttcc
//
LOCUS       DAWEMR010000005        32903 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_550_length_32903_cov_10.023289, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000005 DAWEMR010000000
VERSION     DAWEMR010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 32903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 32903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..32903
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_550_length_32903_cov_10.023289"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9052..9061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12784..12793
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15089..15098
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    27585..27594
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9176 a         7035 c         7750 g         8902 t
ORIGIN      
        1 ctttgtgcgt ttatctaatt ttattattcc tattgacaaa tccacattaa tgagctaaaa
       61 tataaatgtt attattttat atggtgagaa aggaaaacca aaatatgaaa atcaaaagac
      121 tacttgcagc agtgctttcc cttgcaatgg gcgtgtcaat gatgacagcc tgtggcagtt
      181 cggacagctc ctcaaaggct gaaaacgatt cttcagctac agaaaatact cagaagcata
      241 ccgatgaccc tatgacagtg accactgcaa aagaccttgt ggctcagatg aaagtgggct
      301 ggaatcttgg caataccatg gacgccacag gcggctcagg tcttgacgct gaaacctcat
      361 ggggaaatgt ggaaaccaca aaattgatga tagaccaggt caaggacgca ggcttcaacg
      421 tgttcagact tcctgtttca tggggtactc acatggacga gaactataca gttgacgaag
      481 catggatgaa cagagttcag gaaattgtcg attacggtat cgacaacgga atgttcgtta
      541 tcctcaacac ccaccacgag gagtggtata tgcctaagaa agaccgtgcc gataaggata
      601 tagaacagct caaagccctc tggaaacaga tatgcgacag atttcagggc tatgacgagc
      661 acctcgtttt cgagggcgtg aacgagccta gacttagagg cgagggcaac gagtgggtcg
      721 gcgacgctga gtcaagagag gtcgtaaatc agtatgcaaa ggcattcgtt gaaactgtcc
      781 gtgcttctgg cggaaacaac cctaacagat gtataatggt aacgccatat gccgcttcat
      841 catcaagaca gaacatggaa gccctcgaag ttccgcaggg cgacgataag ataatagttt
      901 ctatccacgc ttatctgcca tattcatttg cacttgatac cgcaggcaca gatcagtata
      961 caagcattga ctcctctatc acaacacttt tcaccgatat cgacagcgtt tttctaagca
     1021 agggaatacc tgtaataatc ggtgagttcg gctctgtaaa taaggataat acagaagcaa
     1081 gagttcagtg tgtaaaggac tatctcagca ctgcaaagca gtatggcgta ccatgctgtt
     1141 ggtgggataa cggcacaaga ataggaaacg gtgagaactt cggacttctc aacagatccg
     1201 agggcggctg gtatttccct gaaataatcg acgctatcat ggaaacagta aacagctaaa
     1261 aaaatcaagg acggaagtga tgattccgtc ctttttttgt gcgcaaatta tgtgcagttt
     1321 ttgtggggcg aattgatatc ctgcgagcgg agtgcaatcc tgcggtaaac gggcgaacgc
     1381 tgttcgcccc tacaaaaatt tatattatta tcgtttttta tcaatacatt tatgtagggg
     1441 agcggcgcaa tttttttgtg taggggacgg cgccctcgac gtcccgccac tctcgttcgt
     1501 ttcaatcaca aactgttttg ttcattaaac gccacaaaaa aaggacgacc ccaagtcgtc
     1561 ctttttcata tctattcccc tgaaagggtc tttttcattg caatgtgcgg acagtattcg
     1621 tccttgtaaa catcaccata cgcagtgtag ccaagcttct cgtaaaattg ctgtactctc
     1681 gtttgcgccg ataagccgac ttccttgtag cccacttttt cagcctgctt ttcaagggct
     1741 tccactatca ttctgccaag atctctgcca cgatatgcct ttctcacgca gatacgtcca
     1801 atgtgcgctt cgccattttc gtcaaacagc ctgcctgttg caatgggata tcctcctgtg
     1861 tagataacag catgataagc cttagcgtca atatcgtcaa attcgttcac aaagccctgc
     1921 tcctcaacga atacctgctg tcttataagc ctggcgtctg cgttgtttgc aagccacttt
     1981 gcgatcttta tggtgtaatt atccattata tcagccccca agacgtatca tttcgtcgat
     2041 acattttctt gcagaggtta tctcctcgtc tgtcattttt atctcaaaac cgccgccgtt
     2101 gccgatgccc tcgcaagtct tgtaaacgtc cataagagtg gtgagcttca tgttaggaca
     2161 aagtatcttc tttgagagta tgtaaaactc cttctcaggg aatctgtact gcaaatgctc
     2221 cgcaatagat atctcagtgc ctatgatgaa ctcctttgcg tcagactcag ccgcaaaatt
     2281 cattattcca gaagtcgaac caacataatc agccatttcg cttacagcag ggatacactc
     2341 agggtgacaa agcaccagtg catcagggtg agccgtctta gccgctatca tgtcagcctt
     2401 tgttaccgac gcatgaacag gacatccgcc ctgcaaaagc attatatcct tttcaggaca
     2461 ggcttttttt acgaatgcac caagattgca gtcaggaatg aacagtatct tgtcagcgtc
     2521 catgttcttt actatcttca ccgcagacga ggaagtaacg caaacgtcgc aaaccctctt
     2581 gagtgcggct gtggtgttta tgtagcatac tacctttctg tcaggctcct gcgcctttat
     2641 catttctatc atctcaggtt ccatttgctc tgccattgga cagcctgccg atggatttgc
     2701 aagatatacg ttcttgtcag ggttgagcat ttttgccgcc tcagccataa aatgcactcc
     2761 gcacataagt atgtttttgt ttgagacctt ctgcgcctca acgcttagct tgtatgaatc
     2821 acccgtaata tctgctatct ctactatctc cttagcctga tagctgtgcg caagtataca
     2881 aatatcgttt tctttcttta actgaagaat tttttcctgt acgtccttaa tcatatccag
     2941 atcatgcctt tcgacctttg aaaagcatat cctcctttcg gtgtttcggc atttgccgaa
     3001 agaattatac catattatta tagtatgata tagacatttt gtcaagggca tttgtaccat
     3061 accgcccata gcacttgaca aattcattaa cagggtgtat aatcaacagt gttgacaatc
     3121 aaccgccttg acatttggag gaataacaat gaacgataaa acagcccagc gttttggaga
     3181 agttttcaga cgattttttg aaacaggata tatggacgac cttgcagagc tttttcaggg
     3241 cgaaagcaaa gtgatagtct atctcagcac agccgagggt atagtctatc cctctgatat
     3301 aagccgtgac cttatgatat cacgtcagcg agcaacgtca gtgctcagtg caatggagaa
     3361 aaaaggcttt gttagccttg tgcgtgaccc tgaggataaa cgccgtgttc aggtgaaact
     3421 cctcccaaaa ggtgaaaagt ccattgaaga aaagcagcag cgtcttgaaa aatatttcag
     3481 cgtgttcgtt gacgcattgg gtgaggaaaa tctcaaaaac ctccttgaca gctccgaaaa
     3541 agctgtcgag gcacttgaac atacaatgaa gggcaactct cagaaagtct gaggtgcgga
     3601 aaggaaactg ccatgaacat ttataacgaa tttagaaaaa cggcaataaa ccgctatagc
     3661 aagaccgctc tcgtgatgat gaacgacgag ggtgaaacaa cggaatatac attcggacaa
     3721 actttgcagc aggctgatgt tttttgctgt cgccttaaag ccatgggcgt tgtggcaggg
     3781 gatagggtct gtatcgcctc tgagggcaga cctgagtgga atatcgcatt tctcgcctgt
     3841 gcaaagctaa acgccacagc cgttcttttg gattattcgc ttcctgcggc agagcttgtc
     3901 gcactggtga aaaagtcaca gcctgtgtgc gttgtcgcct ctcaaaaggc ggcaaagaag
     3961 cttacaagcg tgaaaaatat gcctgttctc gatatccaaa accagctcga cagacttgac
     4021 ggttcagggg aaaatatact ccgccaaggt gaagtaggcg accctgatat cgcagtgata
     4081 atcttctcca gcggtacgac aaggacagcc tcaggcatca tgcacaccca cgattctcag
     4141 ataaattcct gcaaaatggt ctgccagtgc aacggaataa ccgataacga acgctatctc
     4201 ggaatcctgc caaacagcca tatctacggg cttttcgcac aggtcttagc ccctctcctc
     4261 acaggtggaa cagtgtgctt tatagagtcc ctctccgcaa agggtctttc cacaggcttc
     4321 caaaacttca agccgacagt tttcccaggt gtgccaaagg tctatgagct tttgaaaact
     4381 cagataatgc agcagatcaa cgcaagcaaa gtatcttcag cactttttaa gaaaatgttt
     4441 cctgttgcac ttcatcagcg taagatctac ggcataaaca gcggcaaaaa gatctttgca
     4501 aagatacata aagccttggg cggcgaactt agatttctct gttccgcagg ctcgccaatg
     4561 tcaaaggata catttgattt cttctacggc acaggcttta atatggtgaa caactacggt
     4621 gcgaccgaaa cgagcatacc aactatcggc acatatggca agcacgtcta tgctgacaca
     4681 tgcggcaagc cgtatcctga tataaaagtt aaaatcgacc gcagcggtga gcttctcata
     4741 aagtcacctt atatgatgat aggctactat ggcgacaaaa agtccacaga tgaagctttc
     4801 aatcctgacg gctggttcaa gtctggcgac cttgcaaagt tcgataagca taaaaatatc
     4861 gtcattctcg gcagatgtaa agaaaatatc gtcctatcca caggcaaaaa agccgcccct
     4921 gacgatatcg aacaggtcta taggggcatc gagagcgttg acgagcttgt ggtctgcgga
     4981 gtgcctgcga aagagggaag ctatgacgag gtacacgcct ttgtggtctg tgctgaggat
     5041 aagcgtgagc agacggaaga aaagctaaaa gagataagct cctccgcagg tcaggcaatg
     5101 aaactcagcg gcatacattt tgtggattct ataccaagaa cagctatcgg caagccaaag
     5161 cgttatctgc tgaagaaaat ggtcatggag gataaccttg acagccttac aaagcaggcg
     5221 aaaaatagta tcgacccgtc ggatatcccg tcaatggtga gaaacgcagt ggcaaaaata
     5281 gcacacgttc agccgaatga agtcaagctc agtacaaaat ttttgcagga gttcaccata
     5341 gactccctct cagccataga gcttgcattg gagatagagg agttcagtgg tgtgcgtgtt
     5401 gacggctgta tggataagga tatgaccgtg ggcaaactta tcgccctcgt gcaaaaccct
     5461 aaccgcataa aaccgcagac tgtgaagtca atgctctatc ctctggacaa gaaaaagata
     5521 gactatcgta tttaccgctt ttacagaaat cttgtgacag cattctacga agtcaaggtc
     5581 accaacgagg aaaatctacc tgataaggac ggttatataa tctgtgccaa ccacgtttca
     5641 aatttcgatt ttatcttcct caccctgaat ttcagatacg agcgttttgc aaaattctgc
     5701 tgtatggcga aaaaagagct tttcaacggc agtttagcca gccgacttat agtaagaata
     5761 ggaggtatgg tgcctgttga caggggcgga agagtagacg aaacaatggc ggctctgcgt
     5821 aaaaagcttg gtcagaaatg gggtgtgctg gttcacccag agggtacaag aagcaaaact
     5881 ggtgaaatgg gaaagttcaa aagcggtgca gccgtccttg ctatcgaagc caatgtacct
     5941 atagtaccag cctacatcaa aggcggacac gagatattcc cgccagacag aaagctccca
     6001 aggcttttca actggaagcg tcttagcaaa tataaagtaa aagtcgttta cggtgagccg
     6061 atttatccta atggcgaaac ccctgaagag cttatggcga aagtccgtga ttctgtgctt
     6121 gccttgaaaa acgaagagaa ctgatttaaa gtgaggagtg aggaatgaca aatgaggaat
     6181 taggaatgag gaatgaggaa ttagcatagc tgtggtttga gatactccaa gaggtaagtt
     6241 aataagacaa ttggtcagtt aattgtgcga acgcagttcg caataaaaaa aatcatcgcc
     6301 gcaggcgata ccttcattcc tcattcctca ttcctcattc aatctcattc ctcactcttt
     6361 ccgtttgaca agtcgcaaaa tatgtagtat aatgtattaa tacgctgata cggtgacgtg
     6421 ttatcgtaat attttgttaa ggaatgaata tttatgactt ttgaggaagt aaaaaaggtc
     6481 acggagatcc tgctggagtt cctgcctaat acactaaagc tttttgcatg gaccatcgtg
     6541 ctttcaatac ctgtgggagt gcttgtgaca atgctgaaaa aatgccgctt caagcctatc
     6601 tcgtggctga caaatctcta tatacttgtc atgcgtggca caccgctcat gcttcagata
     6661 atcgctatct actatgcagt accgcttatt atcgcaagtg ctgcaaagca gggcaagaca
     6721 ggcggtgtat atgacgtgct taccaatata aaaaacaacg agaactatat gtttatcgcc
     6781 cttgttacgg catttgtcct taactatgcg gcttatttcg cagagatatt cagaggcggt
     6841 atcgaggcta tcccaaaggg acagtacgaa gcggcttcaa tgctcggcat gacgaaaatg
     6901 cagacatttt ttagaataat cctgccgcag gtcacaaagc gtgttatccc tgccacatcg
     6961 aatgaggtca tcgtccttgt aaaggatacc tctctcgcaa acgttatcgc attcgctgag
     7021 ataacaatga aggcaaaaca gcaaatgaac ctgtacagct caataacacc gctttttatc
     7081 gcaggcgcat tctattttgt actcaacgct atcgtgacgg tggcatttgc cgttatcgaa
     7141 aagaaattcg actattataa ataaggagaa ctcatatggc tattcttgaa gtgaaaaata
     7201 tttctaaaag ctttgacagc cttagggtgc tcaaagatat ttcttttaac gttgaggtgg
     7261 gcgaggtagt cgcaataata ggtccgtcag gaagcggtaa atcaacgctt ctgcgctgta
     7321 taaaccagct tgaaaaagca gacggcggag agataaacgt tgacgacgtg aatatgttcc
     7381 atacagaaaa tggcaaggca gtgtatgcac cctctaagac cctcagggat atccgcctga
     7441 aaataggtct tgtgttccag aatttcaacc tttttccgca cttttctgta aatcagaata
     7501 taatggaggc gccactccat gtgctcaaac agccaaaagc cgaggcaaaa gccaactgcg
     7561 aagagcttct gaaaaaaatg ggactcgaaa ctaaaggcaa gtcctatccc tgcgagcttt
     7621 caggcggtca gcagcagcga gtatctatcg caagagcatt ggctttgaaa ccgcagatac
     7681 ttttctttga cgagccgacc tccgcacttg accctgagct tacaggggag atattaaagg
     7741 taataaaaga gcttgcaaaa gagaaaatga ccatggtcat cgtcacccat gaaatggcat
     7801 tcgcccgtga tgtggcaaat catgttatct ttatggacga cggctatatc gtggaggagg
     7861 gtactccgga ggaagttttc ggtcagaccc agaacgaaag aacaaagcag ttcttgcaga
     7921 gatttaatga cagataggac gggaaattgt tgtaaaatat aagagatgat aacgttttct
     7981 gtgattaatg cacaaaagaa gtgtgacaat attgttagaa atttcaacac tttttggaat
     8041 aaaaaaagtg aaaacccctt gattttaggg agaaagtctg ctataataag actagaattg
     8101 aatgacaatt cataaaacta tattcatatg gaggagatta acgatgaaaa tttctaagat
     8161 tctcgctggt atgtcagcag cagctatcgt agcttctatg gcaatgatcc cagcatccgc
     8221 agcaggcaca aagctcaacg taagcgatga cctcgctaag atcggcagac agactcagga
     8281 tgaccttttt gctgtattct tcaatggcta tgagtcagag ggtgctgtaa acaaggatgc
     8341 tatctctgac cttggagctg tttacggtgc ttcattcaca atcaaggttg acaacaacga
     8401 ttggaacacg atcggcggtg gcggatcaat caacttcaac tcaaagtctg gtggctggaa
     8461 cgctaaggac tgggctggcg gctcatgggt tgaggacgat aagccaaagg atactctctg
     8521 ctacacaaca gaagaggaag gcacatacgt tcttgaatac ctcggcgacg ctcctgtttt
     8581 cgcaacaaca gacggcgatg ctgataatta cgcagcagtt ggtatcaaca actgggcagc
     8641 agaccttaag ggtatcgacg ttgtaggtat gaaggttctt ggcgctgacg gcaacgttct
     8701 cttcgaggct ggttctaaca aggacaagaa ggaagttcag aagaagccag tagctgagga
     8761 gtcaagctct ggagctgatt caacaccttc aggcacagat tctaagaagg acgattctaa
     8821 gaaggacgac aagtcttctg cagcagctac atcttcaaca gcagctggca caacatcttc
     8881 aaaggctggc acaacaacta caacagctac atcttccgca gcagcttctg acaacacagc
     8941 agctacaggc gcaacagctg gtcttgcact tgctggcgta gcacttgctg gtgcagctat
     9001 cgttatttct aagagaaagt aatctctctc agataacaca cttgaataag tnnnnnnnnn
     9061 nttctgtcat tcgggaagct ctgttttttt cgtttgtgta acctgcacta atttgtaaac
     9121 gatttctgtg caaaacatat gaaatcatgt gcttaaattg tgcaaatagt atattgaaaa
     9181 tatgtgacaa ttatgttata atacaaaagt aaatataggt caggctatgt gtatagcatg
     9241 acaattaaac ggaggttatt aaaatgaaaa agtttttagc ttgcgctctt gcacttacac
     9301 ttacaagtgc tatgttcgct tcttgcggca gctctgatga ttcttcatca aaggctgagg
     9361 caaaaactac tacaacaaca actacagctg ctacaacaac agaggctact acaacaacag
     9421 ccgctcctga gtcaacagct gattcaacag ccgcttccgg cacagagtct gagggtgagg
     9481 ctgcaaagga tatttccgaa atgccagcta ctctgaagaa ctatgaggag gcttctgtat
     9541 atttcaagaa ggatatggac gttgcttcta tcgttgaacc tttcgctgag aaggattacg
     9601 agaacgatga gtctcatgta aatctctcta ttgaggagct tgcaggtatt ccaatgctca
     9661 aggtcgagac tctcgatcag gataatggca actacaagat ccctaagatc aagttccaca
     9721 tggagaagtt ctttgagggt cacgaggctg atcttgagaa gatcttcact atcaaggctg
     9781 acgtagttac aaaggcagtt ggcgagttca caggtgacga cggcacaaaa tcactcgttc
     9841 ctggtaactt catgggtgca ttcgttacac agcctacaac aggtgaaggt gaaaacagtt
     9901 ggaacgacct ctatgacttc ggcgaggctg agtggacttc tgagtggggt tcctatgagc
     9961 ttacaatgag acctggtatc aaggacggcg ctacatttgt aaactcaact gaaccacagt
    10021 atgtttcact tatgagatgg ggcattccaa accaggctga tttctacatt gctgacctca
    10081 gatttgagga tgaggacggt aacgttatcg cttgcgcaaa ctttggtaaa taattcattt
    10141 tcatacataa gaaaggtgca gattttttct gcaccttttt ttatgtgaaa tttacgctta
    10201 cctcccctga tatctccaag ctttttactt ttccgccatt gattttagcc gaaaaatcct
    10261 gcccttttat ctgacccgtg gcggtgatat ctcccttgtc atttttgaga tacgtcacgc
    10321 ctttctgcga aataagcttg tcaagcacag cggcagtcat tgtgacagcg gagctttcgg
    10381 gtatgttacc tcggtcagtt tcgtaggtca aacccttgta ttctagtcgt gcgctttctc
    10441 ctgagcaggt tatcaccata ccgctgacag tttctggcgc agttatctca cactcccaca
    10501 tatctgcgcc ctcacgggtt aagcttgcga cgtattccgt gccgtcaatg gtgaattcag
    10561 ccggggtctt gtaatcctct gccagcttta cgggtttgac ttcggtgctt tcaccgcatg
    10621 agcataagcc cattaccgcc aaaatcgtga gaaaaaatgc ctttgttctt ttcattttgc
    10681 cctccattaa aaaagaccct cttcataaga agaaggtctt tgtttttagt acagcctatt
    10741 cggcgttttc aaggtcatac aaaaatcgtg gcaaagcgtc aataacatct cttgcaatga
    10801 ttccacgctc agatcttttg tatcctgcaa gtagagccgt cgagccaagt gtgaacgacc
    10861 caagtgccgc cgcatcaaga gcatcgcacc cctgagccac ataggaggaa acaatgcctg
    10921 caagcatatc ccctgagcca ccctttgcaa gcgcagttgt gccttcggtg caaagataaa
    10981 cttttccacc gtccacagca aatgtgtcag ccgacttcgc aagcactgtc acaccatatt
    11041 tttctgacag cctatatgca gacccaagtc tgtccgacat gacctcgccc accgtcacgc
    11101 cacaaagcct tgcaagctcc gcagggtgag gggtgaggat aactgtactt ttcttcttca
    11161 acagtacatc tatattcgga caaagtgaat ttattccgtc agcgtccaaa actatgggta
    11221 tctccgcttt ttttataagc tccgccacaa gcttctctgt ctgagccgtg tgaccaagcc
    11281 cacagcctat cagcaaacag cttgtctttt cgcacttttt cagtatcgtc tccgcatttt
    11341 cagccaccat aaagccgtcc ttgtcggttt tcattgccga gtatgtacat tcatacatac
    11401 cagccgacag ggagttgact acgcttttgg cagtgcaaag ctcaaaaagc cccacgcctg
    11461 ttcgcattgc cgccattgct gaaagccctg ccgcacctat atagctctcc gagcctgcca
    11521 cgctcaccac cttgccaaac gtgcccttgt gtccgtcagc aggtctaaaa ggcagtgaag
    11581 cccttgcttt taccacgtca aaaggcgtta taacagggta tagggtattc tcgcaacgct
    11641 cgtcaatacc gatatctttt accagaattt caccgcaaaa gtccaccgca ggctgtaaat
    11701 aaagacccag cttgccacga tgaaaggtca ctgtcacgtc agccttgaac gacaacttgt
    11761 cagcctgacc attccgtgcg ttgactcccg acggcaggtt gcaggctatc ttcatgcctt
    11821 cacatttttt acaagctctg aacactggca ggatattttc acgcaagctt ccgtgaaaac
    11881 ccgttccgaa aatgcagtcc acaagcacgt ctgcatcctc gataaaatcc agcgcatttt
    11941 cagctttcag cacctctacg ctcttatcaa gcctagcata cgctgccgag gagaggtcag
    12001 tgtcaggctc gccacagcat aggaccaccg caggcttgag accttgttcc gcaagtaaat
    12061 ttgccgccac aagtccgtca ccgccattgt ttcccttgcc aataagaagc actatctttg
    12121 aggacgcaga cgagttgctc gcaatgacct ccgcaagctg tgccgccgca ttgtccatga
    12181 gctgagcaag gctcacgcca tatttttcag cctcatgctc gcaaaatgtc atttgctcca
    12241 ctgttaatat cctatccatt tttatcctcc ttttcataag ctatcaccac tgccgaagcg
    12301 atatccttga tgtgggaaag tgacaccgca aaggtcagcc tcctattttc agccgccaaa
    12361 agcgcattcc ccgtaagctc cagataagga cagccaagct cgtcacgcaa gcatgacacc
    12421 tcgttcaagg cgaagccctc gacccctgtg cctaaagcct ttgcgaagct ttccttagcc
    12481 gcccaattcg ccgccatgga ttcgtatggg tttttcttct gtgaaaaaag ggtcagctcc
    12541 ttttcggagt aaaccctttc acagaaacgt cggctctgac agcttttttt tatgcgttcg
    12601 atctcaataa gatcagttcc gacaaatatt ttcattttta tttcctcagt gttcttggaa
    12661 gcgcaggctt cataagctca agcatatcat catctggtgt gaagataaca gttgtaagtc
    12721 cgagattttt gtggttgaat gttatgtagt agtctgtaag gctatcgtca caagcgcagg
    12781 caannnnnnn nnnacatcga gttttgttgt aagataataa ccgccataaa gtgcggcagc
    12841 ggcaagaaaa atgcctatta cagaaaactg cgtgccgaaa gggaagataa agcacagtgc
    12901 ggcaagtgcc acgcttgcta tcatgatgaa aattttctgt atcttatcgt tctttgtggg
    12961 aatcttgcga actatctgtt ccttgaagct gtccattatt tgtcctccgt ttattcagtg
    13021 ccgctgagaa gctctttcat tacccagccg cttttatcgt tgtattttac atatacccat
    13081 atgtttccgt caccgtctgt tctttcgcca tagccgttta tggtcttgcc gtcaggtatc
    13141 ttttctatca cgtcatattt ctttgacgga ccgtatctga gattaagata agaggtgtct
    13201 gactttacaa gaaatgggat agaatcaggg tatttcacat catcacccaa agggaaaagt
    13261 gcgtcctttt tgaactttgt ggtggtggtc tttttagtcg cctttgtttc ggcagatgtt
    13321 gtggttgtgg tggtggtcgt cctctttgtt gccgttgcga ttatgtcagg gtcgtagcct
    13381 tcgtcaatgc ttacgtctgg gttttcaagt gtgcctgccg tcacagaagt ggtggtggtg
    13441 tagccctcgt aatcctcatc gtcctcatca taatcgtcgc ccattgtggc ggtctttgtg
    13501 gtctctataa ctctctgggc ggcagtaccc gtggtggtct ccatttcgga ggagctttct
    13561 ggaagcattg ccccacatga cgcaagcccg aaaaccgcag aacaagccag cacagccaca
    13621 agcagttttg atctcatttt ttatatcctc ttttcagaat ttacacaatt ttattttaag
    13681 ccaagaagtc tttctgcgtt gccgccgaga attttatctt tgttatcctc ggaaatgttc
    13741 aaacgcagta tcttttcttt tataaggtag gaactgtccc aagggcagtc gcttgcaaaa
    13801 agtatcctgt cagccccatg cttttcaacg atagcctgca taagctcgtc aggacacata
    13861 cttgtgaaag cggtgtcaaa ataaacttca ccgtcaactc ctgccaaaac gtcacgcacc
    13921 tgctcccaca tcatgtttgc accaagatgt gcaagaatga ccttgagctt agggtgcttt
    13981 tttatcatgt tcagcgccct ctcaggtgtg gagtggataa ggtcagggga aacgcagtca
    14041 tagccagaat ggaatataac aggcaggtcg gctctttcaa tggcgtcata tatcttgtcc
    14101 atgcgctcat catctatcat aaagccctga taatcagggt gaagctttat gccgtgaagt
    14161 ccaagctcct ttatgtgggc tatctcctgc tcatagtcct cagcgtcagg gtgtattcca
    14221 ccgaagctta taaatcttcc gccgtcttgc tcagcgcacc aatcgtttat aactctctgc
    14281 tgagtaggtt ttgttgccac tgataacagc acgcccttgt caatgcccca ttcgtcaaaa
    14341 cgctgtatgg tttcctctgc agtgcctctt gtgaaaggag ttattccact gcaaacctga
    14401 agcttttcaa cagccttttc cgctaccttt ggattaaagg cgtgaacgtg aaaatcaatt
    14461 accatttaaa acatctcttt ctgaaaatac ttttccatat ggtattatac cacacattca
    14521 agcttctttc aacgaatatc ggcaattttc acagtatttt catctagggc tattcccagc
    14581 ttttccacac ctcggggcag aagcctactg tggctttctt gccgtttctt acgataggtg
    14641 cggcaaaaag ctcgggatag tcaagaagct tttctatctt ttcctcggtg cttccaagat
    14701 attttatgag cattgcctgc tctccctttg cctttgggtc gataagcttg tcgatatcgc
    14761 cgacagccgc aagcactgag ttaagctcgc ccttgctaag cccctttgta ggcaggtcaa
    14821 cagattggaa ttttattcca cgctctttga aataacgctc ggcctttttt gtgtcaaagg
    14881 attttgactt gccgaatatc tgtatgttca tgggattatc tctccgtttc tgcattcaag
    14941 aatgatagta aaaggaccgt cattgagcag tgaaactttc atgtctgcac cgaaaacgcc
    15001 cttttctacc ttgcctgaaa ttttctcacc gcagaccttg cagaagtatt tataaagtct
    15061 gttggcttca tcaggtttgc ctgcatagnn nnnnnnnnat agataaatga cgggcggttg
    15121 ccgtggtgac aatctgcata aagggtgaac tgtgaaacgc aaagaatatc tccgccaaca
    15181 tcgtttatag aaagatttgt cttgtcgttc tcgtcagcga aaatgcgaag ctttgatatc
    15241 ttgtctgcaa gcttgtcgca gtcagcctca gtgtcctcct gacctactcc gaaaagtatc
    15301 agaaagccct ttcctatctc gcctgtgacc ttgccctcga cctcaacttt tgcacttgtg
    15361 accctctgta ttaccgctct catcagccca cctccacggt gtagctgtag gtgtaaacag
    15421 cttcaggtgc aagcttgata gcatgaggct tgttttcaat atcctcaaaa gcgtcgtcct
    15481 cgtaaacagg cacgctagtc caaggctcaa ggcagataaa ctgagcgtca gggttgcctg
    15541 tggaagtcca tactgcaatg cagtcagagt gtgggaatga cagctttacg aaaccgccgt
    15601 cagacttttt cagggtgaca gcctttgaag cagggtggtc tttcatgata acgtcatagt
    15661 cgaaaagctc tcttgtgaga gtgatagtct tttcaccgtc aaggatagtc ctctcgccgt
    15721 ttgggatagg ctgaacgata tgctcgtttt tctcgtactc aactgtgcat ttgccacctt
    15781 ggatatcgca gataaaagca gggtgaccgc cgagatagaa gtacatagcc ttttcatcag
    15841 tgttttttac tgtgtttgtg acggtgactt ttgaaccttt tatggagtag gtgacttcaa
    15901 gctcaaagtg gtatgggtat tgagcaagtg tcacgtcatt gtctttgagg agaaagctca
    15961 gtgaattttt tgtgctgcct gagaaagcaa attcgctgtc acgggcgaag ccgtggtttg
    16021 cagtcatgtg atattcctga ccgcctgcct tgtaggtgcg gtctttaggg gagcatatga
    16081 acgggaagag gataggagcg tagcgtttcc aagcgtcacc gcattgccag agatattctt
    16141 tgccgtccag tttgatagag tgaagctcag caccgaaagt gtcggctgag atagagagtt
    16201 tatcattttt tatttcataa agcatagatc agacctccga gagaaaaatt ttatacctta
    16261 ttattatagc acattgtttt ggagatgtca aaaccaagtg agcagtgagg ttatgaggtc
    16321 ggctggggta tgggcgaaaa cgtttgaaaa aacttaaaag tctgttgaaa attctgttct
    16381 gatatcaata ttttaacagt taaattttat gactttgata cagaataact aaaatatttt
    16441 caattgtttg tgtaagctga caaaacttgt gatgactatt gactttaatt tggagatagt
    16501 ctataataaa atggtaagat tatttaattg tgcagtgctt tttgttcaca gcttcagact
    16561 gcactgagga gaaatgatta tgaatattaa agaagcaaaa caacagatac ttaatgcaat
    16621 acaagcctat ctcattaaag acgaatttgg cgattaccga attagcatag aacatcagag
    16681 acctgtgttc ctgcttggcg cacctggtat cggtaagacc gctatcatgg agcagatagc
    16741 tcaggaaact ggcgtgaacc ttgttaccta ctccatgtcc caccacacaa gagagtctgc
    16801 cctcggtctg cctgttattg tacatagaaa ctacactggt gctgacgtgc agatatccga
    16861 gtacacgatg agtgagatcg tggctactct ttatgacact atcgaagcta ctggcattaa
    16921 agagggtatt ctcttcctcg atgagatcaa ctgcgtttcc gagtcccttg ctcctgcaat
    16981 gctccagttc ttgcagtata aggttttcgg acttcatcag gttccggcag gttgggtagt
    17041 tgttacagca ggtaacccgc ctgagttcaa caagtctgtt tctgagttcg atatcgttac
    17101 tctcgaccgt gttaagaggg tcgagtgtca gcctgatttc gacgtttgga aagaatatgc
    17161 ttactatgca ggcgttcacc cagctatcat atcatatctt gagctttacc ctgaccacta
    17221 ttatatggta gacgcaagcg ataagaacaa ctaccgcttt gttactgccc gtggctggga
    17281 ggatctttct gaaatgatgc agctttatga ggagagcggt atccttgttg accacgctct
    17341 ggctgctcag tatgtacaga acccagagtt ctcaaagggc tttgcggaat attatgacag
    17401 attcaactac tatcgtgaga aatatgacgt tgacgctatc ctcgagggcg gcataactgc
    17461 tcagaacatt gctgacgcaa gagcggcagg cactgaggaa gctgtggctc ttatgaatct
    17521 gcttatggac ggcatcacat acacaatgag aagctgtatc caaatggaga agatgataag
    17581 acttatccac ccaagaatgg aagatatcct tgtgaagata aacaatggtc tttcatgccg
    17641 tcagataatc agtgaacaca tcatggactg caacaagagc cttgacaagg cggtaagagc
    17701 aagaaacatc tcgccatcta acaagaagat ccagcactgg atactccaca atctggaagc
    17761 ttatcttgat aagtgtacca acgagggaag agataacaaa aaccgctgta ctgttatttt
    17821 gcagaactcc ttcaacaatc tgctccatgg cgcaaacaac gttacacagc agtctatgaa
    17881 tggtctgaaa tacgcttttg actttcttga gaatgcttat ggcgctgaca gcaatgttat
    17941 gaagaagttt atcgaggagc tgaaaataaa ttgccacacc acaaacttca tcaagaagta
    18001 cggctcagag cagttctaca gacttgcagg tgagcctgtt cagcagccta cctacaacaa
    18061 tctttatgac cttaatctta ctgattgcct tttagagcag gaataagcat agataaaaaa
    18121 tgaatattta cgggcgaacg cattttgcca tacattgtaa tagttgcatt gtaggggcga
    18181 acagtgttcg cccgttttag ctttataggt agttttcaga tacatatttg ttaatataaa
    18241 aagcttgtaa tttgcgaaaa agtgtgatat aataactaag agtgtataaa cgagaatcag
    18301 tgaggttttt actatgggca agatttatga tttaggcata gacgtaggtt cgaccacggt
    18361 caagacggta attcttgacg agggcgagtt tatttataac aaatatgaaa gacacttttc
    18421 aaaggtaagg gaaacggtgg ctgagcagct tcgcactatc agagagcttt atcctgatga
    18481 caagttcaag atagcaataa caggctcggc aggtcttggc atcgctgagg caagtgggat
    18541 ctctttcgtg caggaggtct tttctgcttt tatcgctgta aacaagaagt atccaaaggc
    18601 tgacgttgtg gttgagcttg gcggtgagga cgctaagata atcttcctca cagggggcgt
    18661 tgaacagcgt atgaacggct cttgcgcagg tggcacaggt gcttttatcg accagatggc
    18721 aggacttttg ggcgttaccc ctgacgagat gaacgacctt gctcttaagg cagaaaagac
    18781 ttatcctatc gcttcaagat gtggtgtttt cgcaaagtct gatattcagc ctcttcttaa
    18841 tcagggcgca agaaaagagg acatatctgc gtctatcttt caggctgttg ttgaccagac
    18901 agtttcgggt ctggcacagg gcagaaagat cggcggtcag gtgcttttcc ttggcggtcc
    18961 gcttacatat ctcagtgcac tgagaaaggc attcagaact actttgaacc tcgacgagga
    19021 acacgctatt cttcctgaaa attcttcatg ctatatggct ttcggtgcgg cacttcatgc
    19081 tgacactctt gctgaggaaa tgactataga cgaagccctt gacaagatag tgaatgcaaa
    19141 ggctactgac aatatcgtgg taggcaagcc gttgtttgca agccgtgagg aatataatgc
    19201 tttcgtggaa agacacaaga agtctgacct taaatatgaa gacatcagaa cttacagggg
    19261 cgacgcttat cttggcatag acgcaggctc gacaaccaca aagcttgtgc ttatcacacc
    19321 tgacggaaag cttctttatc agcactactg ctcaaacaag ggtcagccgc tggatataat
    19381 cgcttcaaag cttgaggaga tctacagcct tgctacccct gagcttaaca taaaggcttc
    19441 tgcggtaaca ggctacggtg aggatcttat aaaggcaggt ctgggcgttg actatggtat
    19501 agttgaaact gttgcgcact ataaggcggc ggcttatttc tgccctgacg ttgattttat
    19561 catcgacatc ggcggacagg atataaagtg cttcaagata aaaaacaacg ctatcgacag
    19621 cattatgctc aacgaggctt gttcatcagg ctgtggctcg ttcattcaga cttttgcagg
    19681 tgcaatggga tatgatatcg cagagtttgc aaggctcggt ctgtttgcaa aagcacctgt
    19741 ggaactgggt tcaagatgta cagtgttcat gaactcatct gtaaagcagg cgcaaaagga
    19801 cggagcttcc gttgaggata tctctgcagg actttcttcg tctatcatca agaacgcagt
    19861 ttacaaggta ataagagcca agtctattga cgagcttggc aagaatatag tcgttcaggg
    19921 cggaacgttc cttaacgacg ctgtgctcag gtcatttgaa atggagcttg gcagaaacgt
    19981 tataagacct gccattgcgg gacttatggg tgcgttcggc tgtgcgcttt ttgcaaaaga
    20041 aaaatctcag ggcagggacg gcaagtcagg cgttcttaca aaggctcagc ttgaagaatt
    20101 tgcttacaat tcaagagccg ttcagtgcgg tggctgtggt gcgcactgta accttactat
    20161 aataagattt aatgacggca gaagatttac ttccggaaac cgctgtgaaa agggtgcagg
    20221 cataaagata accgacagaa cggaaaacat gatagagtat aagtataaca agatactctc
    20281 ttatgctgac aacaagcctg caaagcctat tgcaaaggta ggtctgccac ttgcgctgtc
    20341 ttattatgac cttatgccgt ttttctcaac actgctcaca agccttggct ttgaggtggt
    20401 gctgtctgag gagtcaacga gagatacata ttacaagggt cagcaaacta ttccgtctga
    20461 tacggtatgt tatcctgcaa agctttgtca cggtcatata gagagcctcc ttgacaaggg
    20521 cgtggacttt atattctatc cttgcatgag ctacaatgct gacgagggtc agtcggacaa
    20581 tcactataac tgtcctgttg ttgcttatta tcctgagctt ctaaaggcta acaatgacaa
    20641 gctgaatgat gataatttca tcatgcctta tcttgacata aacatcaaaa agcacgttgc
    20701 aaaggcaatg gcaagagcgc tttcaaagta taacatcact gcaaagcagg tgcttggagt
    20761 gctaaacaag gcttattctg agcaggtgag ataccatagg gatatcgagg ctaaggcgca
    20821 ggagataatc gctgaggcaa gggcaaaggg tcagaaaata atcgttctgg caggcagacc
    20881 ttaccacatc gaccctgaga taaaccacgg catacacaag cttatcgctt ctcttggttt
    20941 tgctgttatt actgaggaca gcgtggcgat gaaagtggat acacccgcac ttgacgtact
    21001 caatcagtgg acttatcatt caaggcttta cagggcggca gagtatgtat gcgagaatga
    21061 tgatatgcag ctcgttcagc tggtatcctt cggctgtggt atcgacgctg ttacaacaga
    21121 cgaggtgaga gctatacttg aagaaaaggg caagctttac actcagctaa agatagacga
    21181 gataacgaat ctcggtgcgg ctaagataag actcaggtct ttggcggcgg cactggagga
    21241 aaaagaagcc agcgcaaatg ctgaaaagaa aatggacaag gaggcaatgt aatggctgaa
    21301 aaagagataa aacacgcagg ctcggacaga gtaaaaaggt cttattttga caagtcaaga
    21361 cgtgaggaat acactatact tatacctgat atgcttccta tacacttcaa gcttatcatg
    21421 gcgatataca aaaaatacgg ctataacatg gagcttttgc aaaactgctc acgaaacgtt
    21481 atagacgagg gacttaaaaa cacacataat gacgcttgct atcctgcgct tcttgttatc
    21541 ggacagttta tggacgctct caaaagcggc aagtatgacc ttgaacacac ggctctgctt
    21601 atgtcgcaga cgggcggcgg ctgtcgtgct tcaaactata tccaccttat aagaaaggcg
    21661 ctgtcaacgc agttccctga agtgcctgtt ctgtcgctga atttcagcgg acttgaaaag
    21721 gacgcttcca tagatatcac ccctgccatt gcaatgaagc ttatttatgc ggtactttac
    21781 ggtgatatgc tcatgctttt gtacaatcag tgcaagcctt acgagataga gaaaaattct
    21841 acggacgagc ttttggcaag gtggcagcac aggcttggaa agctgtttgc taccccgagc
    21901 agatatatgc acacaaagag catttacaat gctatgctca aagactttgc ggcacttcca
    21961 aggacaaagg agaagaagcc taaggtaggt atcgtgggag agatatacgt taagtattca
    22021 ccccttgcga acaatcacct taacgacttc ctgctgtctg agggctgtga gcctaacgtg
    22081 cctggactgt tggacttcgt gctttactgt gcgtctgacg ctatcaatga cgaaaagctt
    22141 tatgacaaga aaaccagccg cactcttatg tacggcattg gttatgatgt gctttacaag
    22201 ttccagaagc agcagataaa ggtcatcaac gagcagggca ttttccagcc gcctcatgat
    22261 tttgaacacc ttagaaagtg tgctgataag tatattagtc agggcgtaaa aatgggcgag
    22321 ggctggctca tcactgctga aatggcggct cttgcagaaa caggcacaaa gaacataatc
    22381 tgcactcagc cgttcggctg tctgcctaac catattgttg caaagggtat gtcaagaact
    22441 atcaagcagg cttatccgga cgcaaacata gttgcaatcg actatgaccc gggtgcgaca
    22501 aaggtcaatc aggagaaccg tatcaagctt atgcttgcta atgcggtcat ggattaatag
    22561 agaaccgaaa gataaaaccc cctcggagct tggctcggag ggggttgttt tgtgcaaaaa
    22621 gcaggcttaa taattgtagc ttttctgttc atgtatctgt tcaggtgcga taaagctcag
    22681 ggctttaagt ttctcggtga ctttatccgc ttcggctttt gatacgttga aatcaaagtc
    22741 gatatagtcg atatcatcgt tgttatatat atcgtctgta tcgtaagtcc ttattattat
    22801 gcttactata tttacttggt tctgtgttct tttaaagtgg tgtatatagt cgctgatact
    22861 ctttttgcgg ctgtcaaagg cgctgttgta gatatctatt atctgcatgg tctcgtcttt
    22921 tgtgagggtg tttttgtcct gcggattttg gtggcttgaa atataggaag ctgttatgtc
    22981 atctgttgtt gtgttcaacg cccacacatg attgtattca gtaagctttg cttcatctat
    23041 cttgcctgta aagccattgc agaagtatgc cagaatgttc actccgaaaa tgattaccat
    23101 atacactgcg aaaatgccta cccatttcac aaagcggatt attgtaagct tgctgtttga
    23161 tgatagtatt ctcacaagga gatagcccaa gagaagtgca ggaagaagta tcaggttgct
    23221 tgtgtcggag aggacgtaag tcgttacagt gactacggct aggataagta taatttcacg
    23281 ataggcactg aatatgaatg gcttgctcgt gtccttgccg tcacgctttt tgtagaggaa
    23341 atacaaaagt gtgataagca gagctgagaa aattgtgctt ccaatgagaa gaagcaggac
    23401 ttttgtggag tagccaaatt ccataagctt ggcggaaatg gcgctccatg caggacaata
    23461 ggtaacgaaa ttttcaatat cgtatgggta ctgaactgta aatggactaa ttgtggaata
    23521 gtatatgcaa gcagggagag caacctcgca ggctgttatt atcaccgctg tgtagagcat
    23581 ttctgcaagc ctgccgcagc atatcatgca caaaagcacc gcaagtatca caaaaaggct
    23641 tgtggaaagc tgtaagaaca taaatacggc ggaatatctt gccaacatgt tcagtgtgat
    23701 atccgttgtg aggagtatgg cgataaactg aatgatgttc cagaatatca caggcaggat
    23761 atgcagcttc agcagagtga gaagctttga gaagaagcgt tcttttgcac cgactgggag
    23821 agaatacgca aggtctgcgt ggggtttgct gaaaagctca cggaaaaggg tgaaaactgt
    23881 gaacactgcg cacagcgcta tcgccgcaat acatacatat gcaatggcat agccctcata
    23941 atagttcgtt tcgctttcga tgtagtgggt gatgaacttt cgtatggaca tctgcatgat
    24001 aaagttcatg aacagcacaa ggtttgtgat gacgaaaagc agtttgttca ccttgaagtc
    24061 acggcgtgag gtctctatta tcctattctt cgctgtttgc atatccatgc acctccattt
    24121 cgtatatgaa gatctcctcc agggtgaggg ggacaagctc taaaacggca ggggagaggg
    24181 cggaaagctt ggcggtgata tccgccttgt cgccctttgt tatcactgtg gaaactctgc
    24241 ccaaggtctt gaagctcagg acttctacgc ctgcattttc tataacagtg cggtcaagct
    24301 caccgtcagg gagggctatc tgcacctttt caaagttctc tcttacatcg tcgatatcct
    24361 tggcgaaaat gacctcgcct ttgtgtagga gcattgcctt gtcgcaaagc tcgcctattt
    24421 cggtaacgtt gtgggaggac acgataaggg tcgcaccacg gtcgaaaata tcgtcaacta
    24481 tcatggtgcg gataagtctg cgcatggcag ggtcaaggcc gtcaaaggct tcgtcaagga
    24541 ggatatactt tgttcggctg gcaagggcgc aggctactat cgcctgcctt ttcatgccct
    24601 ttgaaaactg agacagcggt ttcttccgcg gcaggtcaag ctttgagata aggtcatcga
    24661 acaccttgtc gtcaaaggtc gggtagctgc tttggtaaaa gcttttcagg tcattcactg
    24721 tcagtcttga aaactgcacc gtttcgtcgc tcacaaagaa gatgttctcc ttagcctgcg
    24781 gccggtcata gacctctgtt ttgtcaatgg tgacgctgcc gctgtcgggg cggtagatcc
    24841 cgcttataac acgcagcagg gtggatttac ctgcaccgtt cgagcctaca aagccgtata
    24901 tgcagccctc ggggatatcc agggtgacgg ctttcagtgc ctcgtgatct tcgtctgcgt
    24961 ttttgaatgt aagtgacagg tcttttattt ctatcattct ttgtcactcc tttctttctg
    25021 ttcctccgcc agttcttcaa ggacggaggt aagcgatcca aggcttatgc ctgcctgcat
    25081 tgcggcggcg gcatggagct taaattcttc cagcaatttt ttctctatag agcctttctg
    25141 ctttgccaca aagcagcctt ttcctgccac agaatagatt atctcgtcat gctcaagtct
    25201 gctgtaggct ttggcgactg tgtttggatt cagacccagt tcctttgcca acattcttga
    25261 gcttggcagc ttatcgtttt ctttcagcac gccttttgat ataaggcttg agaactggct
    25321 gcatatctgt tcgtagatag gtgctcttcc tgtaagatca agcgttatca ccttgaatac
    25381 ctcctttcga ttttgttgtt tcgtttcatt tgttctgctg tactaaatgt attatctgtt
    25441 gtagtacgct tagagtatac aactataagt ctgatttgtc aaggagcgtg gatatttttc
    25501 agcacttatc acaatactgc gtcgttttga tagttggaaa tgtacaaaag tttaggcctt
    25561 ggggagggaa atattattaa aatgtaaaaa tatatttaaa atcttcttat tctattgaca
    25621 tatagcaaaa gcgatgctat actttaatac ataaaagctt aaaagcataa aatctttaaa
    25681 gtgataaatt ttatacaggc gtgataagta cctgcttttg gaaaggaagt aatcacaatg
    25741 gcaaatcagg caaagacgat aaatcagaaa ggtgacctta tgtcatatcg tcctgatata
    25801 aagcttctgg acgcaactat ccgtgacgga ggtcttgtaa ataattttgg attttcagac
    25861 gagtttgtaa aagaactcta taaaactaac ataaaatcag gcgttgaata catggagttc
    25921 ggctacaagg cttctaagga gctttttgac gtcaaggggt tcggcaagtg gaagttctgt
    25981 gatgaggagg atatcagagc tatcgtgggc gagaatgaca gccctctgaa gctttctgtt
    26041 atggctgacg ttggcagatg tgatttcaag aaagatatcc tgccaaagag cgagagcgtt
    26101 atcgacatga taagaattgc aacatatact catcagatgc ctggtgcgct ggagatgata
    26161 aactactgcc acgacatggg ctatgaaact actatcaaca tcatggctgt ttctgcaagc
    26221 cgtgaggacg atatcgcaca ggctcttgac cttgtggcaa aaagccctgt tgacgttatc
    26281 tatcttgttg acagctacgg ctcacttttc cctgagcagg tgagaagact tactgcgcag
    26341 tattgcgagg tggctgaggc tagtggcaaa aaggtgggcg ttcatatgca caacaatcag
    26401 cagctcgctt ttgcaaacac tatcgagagc atttctcacg gtgcaagctt tgcggacgct
    26461 acaatgagcg gaatgggcag aggtgcagga aactgcttta tggagcagat gctcagcttc
    26521 ctgagaaatc ctaactacaa gcttactcct acactgaaat ttgttgaaaa gtacgttgct
    26581 gaggagaggg caaagggttc acagtggggc tatgacgtgc cttatatgct cacaggtgcg
    26641 ctcaaccgcc accctcgtgc ggctattgca ttcctgaagg ataacagaac agattacaac
    26701 gaattgtata tggatcttct taacgagttg tcataaaatc attcttcata taaacggcgt
    26761 ttccatagtg atgtgggacg tcgtttttgt gttgcataat tttttatcat atgttataat
    26821 tattttatgt tttcataaaa ccttttggcg gatatctcgt cattatagat gaaaggactt
    26881 tcaatgaagt taggcgagcc ttgtgaggtg ttacctaaat gtaagggggc ggaaaaatgg
    26941 aggacagcag gataattgag ctttatgaaa gcagagatca gcaggctatc tcacaatcgg
    27001 acataaaata cggcggatac tgttattctg tgggctacaa tattcttgga gtcagggagg
    27061 acacggagga gtgcgtcaat gaaacatggc tcagggcgtg gaactcgata ccgccgcaga
    27121 aacctaaaat gctgtcggca tttttcgggc ggataacaag aaatatcgca tttgacagat
    27181 accgtgcgtc aaagaccgca aagcgtggaa aaggcgagat agcggcggta ttggaggagc
    27241 ttgacgagtg catacccgac aaagacacca ccgagagcct ttgcgacggg atacacctgg
    27301 aggggcttat aaacgaattt cttggtgagt tgcaggagag agacagaaat atatttctcc
    27361 agcgatattg gtttgcagtg cccataaaag agatatcaaa aaagtatgct atgaacttga
    27421 acacagttaa ggcgagcctt ttcagaagca gaaataagtt aaaggctttg cttgaaaagg
    27481 agggagttct tttatgaaaa caacaggaga ggatattttc aaggctttgg aaaacgttga
    27541 cgaacattat atagaagaat gtcagcagag cggaagcgga gtaannnnnn nnnngtgtgg
    27601 aaaaagtggg tggctgtggc ggcttgcgga gttatcgcta ctgtgacggg cgttatgatg
    27661 tttgcaggca ggtcggacaa agatataaag gcaaaggctt cggctgacca gcagatggca
    27721 gacgaggaag caacagtgtc atatagagtt ccttgggacg aatatgcaat gaatgcaaaa
    27781 tatgagacgt ttgaatataa cgatgtggaa tatgtttcaa tgggacatgt tgataagtct
    27841 tttcttgaca gcaagctcac caccatgact cttgaaacgg aaaactatga gacaaacaaa
    27901 aaggaaacaa caaaagcgga tatttattca ataaaggact tgtcaccaag cgtttatttg
    27961 gcggtggtct ttgatgacgg ctctgtggcg tggtatgatg attggaaata ctcacctgat
    28021 tgcaagaccg atatgaccct tgatgacata ataaatgacg taagtcttga aaaaacggct
    28081 catgtggact atgcttacaa tgatgcagaa acgggcaggc tcagaaagta tactaatact
    28141 gacatctcaa agctgcttga cttgttaaag gacgataatg gttcatacaa tgcggtctat
    28201 gagggcgatg cttatgagag cgaccctgat attaatttgt cggtaacaaa tgatgatgaa
    28261 tattacattg agggcggaat aacaataagc ttctatgtag aaagacttga cgccaacggt
    28321 acggcttatt ttactgctaa cggagatctg tatgttcagc tttttgcagg agatgtatta
    28381 aaatttgaca taggcggtga aaaggcagaa aagcttgaaa actggctcaa tgagaactgc
    28441 gaatacactg acgtaaagaa aaacgctgag gtcgctgacg atgttgaccc tagtgagaaa
    28501 gctgtgactt ctgttgccaa ggacgattct gatatggctg acgaagttga tattggatat
    28561 atcgactatt ttgaggacgt tggggacaat acctttacaa caaaggacgg caggctcacg
    28621 gcgaaatact cctatgaggt aagcggaggc gagttcataa gccatattac ctacacaaac
    28681 aactgggaca aggacatggc gcttgacttc acgactgacc agtatatcac cacagaggac
    28741 agtaagggca attccacgag ctatggcgaa aatggcgaca gaaacaccat gaacactttg
    28801 ctcaaagccg gagagagcta cacgtatgac aacaagacgg atattggtgc cgactacgtg
    28861 aaaggcacgg caaagataac gcttatcgca aacactgcaa gcgataaaaa ctgcaatgga
    28921 gtaaactatg tgacgctaaa agatatagag atagattttc agtaatgaaa tagtttaatg
    28981 tatagtggag aactgagttt tttcgataaa aaagataaaa gcctgtcgta tcggactgat
    29041 acggcaggct tgattttatt tgtgcttgta gaccacacgc tcttttacgt ctatctgctt
    29101 gtaggatatt ttgaagccta tccatgtggc gaacggatag agaagaggga atatggcggt
    29161 catgatgaaa acaaatgggg acatattttt cacgtcggca gagtgggcag cccaatctat
    29221 aagaggatag aagcaggcgt cgagaagctt ataggcaggc atgaagttga acgaaccgct
    29281 gaattttgat atcatcagca gtatcattgt tatgtagcat ggtatgcttg caataagacc
    29341 aagtgtgaag ccatagttag ggcaatcctt ttcaccgtgg aatgtgacct tatttctggc
    29401 ttcctctccc tgtttcagac caaagtcagc cataaggcag accactgtga gaagtcctgt
    29461 aagaccgaaa attatatttg ccaatgcacc gaaaggcttt gcaactgcga taaaaaagag
    29521 aaatacgaaa attccgaaga actctgtttc cacccatttc agcagtgcgg tgagtatggc
    29581 tttttgtttg ttgtattttt ccattttaat actcccgata aaattagtat aagatgatta
    29641 taccacgtta tcgtgatatt ttcaactgtt atgcagattt ttcatctgat aatcacaaaa
    29701 gcttcgggca ggataataac ataaaaagga gagtgatagc catgaaaaag cgtgacaaga
    29761 gccgtttccc gcaggcttca gacggcacaa aggtatacaa gccccatgac gttatctcgg
    29821 caggtcagga atatgttgtg acggacaatt acactgcgcc aaacggcaga gtatacaatc
    29881 gtcaggagca gaatgcaggc tgggcgagaa aatgggtaga cgagaacgaa aagtagccgt
    29941 tgaaaaacct ccgcaggtgt gatataatta cagtatatca tgatctgcgg ggggtatttt
    30001 tatgaaaaag acagcattgg taacaggtgg ctctggcggg ataggctcgg agatatgcag
    30061 ggtgcttgct gaggacggct ggcaggtggc tgtatgctac aagaatggca gggcagcggc
    30121 ggaagctttg gtatctgagc ttaaagagcg tggcttatgt gcagaggttt tttactgcga
    30181 cattggcgac aaagacagta tagataaatg cgtggcggaa gtcagggaca gtttgggctt
    30241 tgttacgctt cttgtaaaca acgcaggcac agcggatata gagcttttca ctgacatgac
    30301 tgacgagaag ctatgcgaga tgatgaacgt gaatctgctt ggcgcaatgc gtatgagcag
    30361 ggcggtgctt cctgagatga taagggaaca cagcggaaat gtgataaata taacctcagt
    30421 atggggcgaa aagggagctt cctgcgaggt cgcatactcg gcggcaaagg ctgggcttat
    30481 aggcttcaca agggctcttg cgagagaagt tgctataagc ggtataaggg tgaactgcgt
    30541 atcctgcgga atgatagaca cgaaaatgaa cggcgagctg acggatgaag atgttcgtgt
    30601 gatagttgat gacataccgg caggcaggat aggcaagccg agagatgtgg cgaatgttgt
    30661 gcgttttctc agtggagagg gagcagatta cattcacggg caggtcgtca gagtggacgg
    30721 gtgctggata taggtcactc gccgtgacgg gcattttacg ctgcaatttg tgatatgatg
    30781 taattttgca ccgctttttg aaatgtgtag gggagcgatg ctgtggttgc catatccttg
    30841 atgccaaatt atttttgggt gacattgtgt gatatttttc aacaattcaa cgggcgagaa
    30901 atatcgggct gtcgcatttc ggtgaaaccg aaattgagcc aagcccctac aatttcgaaa
    30961 tttttacaag ttctgagaac gggcgaacga tgttcgcccc tacagattat tctggcaggt
    31021 ttgttgtgtc gcaaaatcgc tacgctcggt gcttgtgcac aactttggtc tgctatgcca
    31081 attcctcact tctcattcct catttctcat tcctcactcc tcatttagct tcgccgtgta
    31141 tactctccac acacatctgc atatactaag ttaaaacatt tctccccatc aggacaagga
    31201 ggtcagtata tggattatct tatgcttgtg gataaattca atcccataga tatgggattt
    31261 tatgatcgtg tggagttgac agaggtgaac ggcaagttta tggaatctca ggcaggaaac
    31321 aggctcaggc ttctgctcaa aaaggcggaa gctgacggca taaagctgaa aattatttcc
    31381 gcctatcgct cttttgaata tcagcagatg ctttgggagc gtgaagtatc tcacgaaatg
    31441 tggggcggtc tgagttatga aaaggctgtg gaaaaggtgg gcagaactct cgctctcccc
    31501 gggtcaagcg aacacaacac cgggcttgct gtggatctcg gcagagaggg tgacaatgat
    31561 gtgagcgacg atttttacaa gacgcctgaa tctcggtggc tttgtgcaaa tgccgcagat
    31621 ttcggcttta tccttcgtta tcctcgcctt aaagagcaga tgaccggcat tgattttgag
    31681 ccgtggcatt atcgttatgt gggcgttgaa gccgctcgta tcattgccgc aggcgggctt
    31741 tgccttgagg aatttctgca cttttactcg gaaaaatata tctgaaaatc ctgtgaaaat
    31801 aattgacaga aagagcattg tgtagtataa ttataacaaa gctatgagcg atagccgcaa
    31861 gtttttgtat gctattgcga aagaaaggaa gttttgttat gtcaaactat tcaagaaaaa
    31921 agtcgttctg gactttaatg ggtgcgatga tacttgttgt tgcgtcaact gcgctttttg
    31981 tatacaaata ttttgaggat aaggagcatg ccgaaaagtg gaaagactat gaggagtgcg
    32041 gtatacagta aataacagac agctttaaag gcactgcttt ttgtgagcag tgcctttgta
    32101 tatcaacaca attcattgat gtaaagttta tcgaaatcgc tgaaaaagcg tgcttgcact
    32161 tgaaatgcag tttgaaatgt gatataatag tcgtaatgac ctgatgacaa acttgttcta
    32221 gggagggacg ttataatgca gataagagaa tcagctgaaa attatctcga aacgatactt
    32281 attctcagcc agcgcaaggg caaaggcgag gtaagaagca tagatatagt aaacgaactt
    32341 gagttttcaa agccgagcgt aagtgtggca atgaaaaatc tccgtgagaa cggctatata
    32401 actgttgaca aggacggtta tattcgtttg acagacaagg gcttggagat agccgagaaa
    32461 atgtatgagc gtcatacgtt gctttcacag tggctcataa agcttggtgt tgacgagaaa
    32521 gttgcagtag aggacgcttg ccgtatggaa cacgttatca gtgcggagtc ctttgcggct
    32581 atcaaaaagc acacaggtat ggcttgataa tatgataatt gccgtgggtc ttcctgcggc
    32641 atttttattg caaacaaaaa ggagaacagc ttgtgccgtt ctcctttttc tgttatttga
    32701 atttgtcttt gagcttgtca aagaatgttt tgcgctttgc gtagttcttt tcaccgaggg
    32761 acttttcgta gtcacggagc ttttctttct gctctttggt cagattgcgt ggaacttcaa
    32821 ctgttacacg aacgtagtgg tcgccgtggt cgtttctgtt caccttcttt atgcccttgc
    32881 ctctcagacg gaacactgtg cct
//
LOCUS       DAWEMR010000006        32614 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_559_length_32614_cov_11.847108, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000006 DAWEMR010000000
VERSION     DAWEMR010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 32614)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 32614)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..32614
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_559_length_32614_cov_11.847108"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15863..15872
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    30087..30096
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9015 a         7819 c         6618 g         9142 t
ORIGIN      
        1 cagaagataa agcaagggcg ataacaattc atatgcaagg actatctgat gaggcgtatt
       61 atgttgatct tctaaagata acaaaataaa aacatataaa aaacacggct gagaaaatat
      121 cagccgtgtt ttttatgcca atttttatag cttacagcca agcgccgtaa atagtttcgc
      181 ccgtcagttc acggtatgag cgcaccttca ggccgagcat atcagctctc gcctttgcga
      241 ttacctgtgc aggtgaattg tagttcacca ctgtggcagg ctcactgtga gaagtatagt
      301 caagcttatc ctccaccgcc tcggctacct catagccctg ggctttcatt gtaggcttgc
      361 ctgacacgat agacttgttg ttaagctcct gcggttttat gaccatcaca ttgtcagact
      421 ttagtgttat gtcaaactga aaatccgccg agtcacaata ctgacccatg cctataaccg
      481 tcacctttgc agttccaacg ttcacgttgt cagagtaaac tgttctgtaa tcaacgtcag
      541 gaataagcgt ttctccgtct acaaaaacgt caaatatctg tgttctctcc ttgccagtgt
      601 atgacataga gccacgcata tttgtggtac agcccacaat agtgagcttt gtttttatgc
      661 tcatatccgc actgtcgctt ccctcttgga cactgtcaac agtgtcaaga aaagctttca
      721 ctttataaac atatcttgtt tttggaacaa gtccatagtc tgtaaatgaa gtgccgtctg
      781 ttacagttgc tatctgctca aactctgcgt cctcagcgtc ggtctttctg taaacataat
      841 agccgtcagc ctgcgacacc tcgtcccatg ttaaagttag cttatcagag cagatatcct
      901 gcgactttac gttatcaggt gcgttcagaa gcactgccgc actgcctgtg acgtaaatat
      961 cgcccttgct gttgtcagca aagctgtttc cgcttatttt aacctgagat ttcttattag
     1021 cttttattcc gtagccttta tttttattgc aggtgttgtt gaaaaactcc acagaagcgc
     1081 tgttgtcagc cataaaaccg ctaagaccgt tttcttcaag gacattgtca gaagccttac
     1141 cgcttccgcc agtgaatata agtccatgat ttttagactt cttaacagtg ttttttctca
     1201 catctgcctt agcttctttg attatcttta tgccgttgga gcctgctgag gttatagtat
     1261 tgcctgatat ggtattttta tcagaaccgc ctgaaacata aactcccgac acgcctggct
     1321 ttttaatggt atttgagctt acctcattgc ctgaaccacc gcttatataa acgctgttct
     1381 gctttgtgcc tgtaagctta tttttggtca cagtcacctg tgacgattcg ctgagccata
     1441 taagatccat ggaataatta tatctgtcat agtcatagct tatatcgtta gaacctatga
     1501 aaattcccga aacgtccgtg agccttactg ccgaaccagc tgtttttata gtgttgtcta
     1561 ctatcttcac accctttaca gtgtaattgt aatcagggtg tgagtatgac ttttcaagct
     1621 tcttgccgaa tatctggata gcatacggct ggggataata atatgtaccc acaacggtta
     1681 tgtcgttgtt tcttattatg gtgttggcat tatcgtcaag acgatccttt atgcctgcat
     1741 agcctgaatt aggcacatgg aaatttgcat tttcataatc ggtcatgtac ttgaaatcta
     1801 tggcacagcc aacgtttttc atagtattgt tttcgattat acatttttta tgattaataa
     1861 gtacgatagc tgttccgctc atattataaa atgagttgcc tgatataagt gtattagtat
     1921 aatatacacc aagacaagct gaatgtgagc caagacctct tataaggtca tggaatttgt
     1981 tgttctttat tacagcattg tcgcaaggag tatcatcaaa aggctcatac gccgggaaaa
     2041 catcactgtt acttatggta tccagctgta tagcctcttt ctgaaatgtt cccgtataac
     2101 catgaaaatc gcaatcctcg atagtgatgc cctttacacc gcctatctca acatgatgac
     2161 cattgtaatt gttttttatc tcaacgttct ttatccaaag gtttttcatg tgaccaaatc
     2221 tcatattaga aaatggtctt gaagtaggcg aagggttgcc gtcccaacag cctccctcga
     2281 taacgatatt tctctcgctg ccatatccgc ctgcaccgtt tgcctgagcg ttctttatca
     2341 tacagccctt gtcaaagttt ctgacgatag tcgctccgtc ctcaagatac agccaagtgt
     2401 ttgaatatat ctccaaaagc ttgtctatcc tgtattttcc cttcggcaca acgaccttta
     2461 tctgcacaga gttggagccg ttgtcaatgg catagtcaag ggctttctgt atcgcctttg
     2521 aggagtcttt ctttcccttt ttgtctgcgc cgaagtcaac aacattcact tcaagcgctc
     2581 ccgaatatcc gcttatttcc gctccactgg cttttgtgcc ggtgatagtg gaataatcat
     2641 aagcaaagca ccttactgtc atgagcataa tggcgagtaa acacgccgct gcagaggcaa
     2701 taaaggtgcg agttatcttt gtcaattctc atctgtcctt tcgttgtctt agggttatcg
     2761 ctgctctctt tttgttcttt ttatcttttt agcagaacat attttacaca tattcaatta
     2821 taccaaatca tgccccgttt gtcaaccgaa aaacgtcaaa aagacgaaag caataactca
     2881 caaagtcaac acatgatact tgtgcgttat cacaacaaaa aaactgcggt gaaaaatctc
     2941 actgcagttt tttaacacat atgacttttt atcctagtgc cacatcaagt atcatcataa
     3001 gcaagaagcc tagcattacg ccaactgttc ccatgtgacc ttcccccaga tgtgcttctg
     3061 ggataagctc ctccaccacc acatagagca ttgctcctgc ggcaaaggca agaagccacg
     3121 gaagaagcgg ttggatagca cctgcggcaa gaacggtgag aagtccgaaa acaggctcaa
     3181 caattcctga aagagtgcca agaccgaatg ctttcgccct gctcataccc gattgcctca
     3241 gcggcagtgc gatagccgca ccctccggaa agttctgtat tcccatgcct atggcaagtg
     3301 cgatagcaga agccagcagt accgagctgt cattatgtgc cgccagtgca aaggcaagac
     3361 ctactgccat gccctccggg atattgtgca gtgtgatagc gaaaataagc attcccgaac
     3421 gctttagccc cctgcggacg ccctcttccc tttcaggctc aggtaaaaaa taaggcattg
     3481 ccttgtcaag ccccataaga aacagcacgc ctagcgcaaa gccgcctgcg gcaggtatcc
     3541 agcctatcct gccgagagct tccgcctcgt ttatggcagg gataagcaag ctccacacgc
     3601 ttgcggctat cataacgcct gcggcaaagc ctaaaaaggc tttctgcacc ttttcgctct
     3661 gcccctcttt tctcatgaaa aagaccacag ccgcacctaa cgtcgtcata gagaatgtaa
     3721 agcccgtgcc tgccgcagcc caaagtagac cattcatact attatgtttc ctttcagaca
     3781 gtattatgtc agatacaatg acatatctta gtctatgaaa agcctttacg ctgtgtgaaa
     3841 aagcctctga cgacaaaata cattaaattt gtctgagatt ttcagaatat gacccgtccc
     3901 aagtgaatat aatgttctca gcagggcatt acaagccctt tacgatcgta tgatcagttt
     3961 atttttagga ggaaacagac aagatgaaca ctatggatct taagcttaac agagagatgc
     4021 tgtcaatgac acggactatc cttgacgcct cctgcgagca ggctgtggaa aaggattttc
     4081 tgctgcccga ctattatccc gatattttca gggtactaaa atgcgttatc aagccaacag
     4141 tacagagcca ttccataaac ggcggaaagc tcagctttga catggcagtg ctcataagag
     4201 tgctttacat cagccagaac gacaagcgga taaactgcat cgagcagaaa atgaactaca
     4261 caaagtcttt cgacctgcaa ggggattgcg gcgaccctat gatatggctc acgccaaaat
     4321 gcgattacgt caactgccgt gtggtaaatc aacgcagact tgatatcagg ggtgctgtga
     4381 ctatccacgc aaatgttacg ggcgaagtaa ctgtgcctat agtcacagac gcattcggct
     4441 gcggcattca gcttaaaaaa gttgctgtta cataccctgc aaaacgtctt accgctgcaa
     4501 agcgtatcac actaatagag gaattgcagc tttctgcggc aaaagcccct gttggcacta
     4561 tcctgcggac agattgccgc attatcccac aggaacacaa gatgatagca ggcaagcttg
     4621 tgacaaaggg cgacgctgag ataatgatgc tttactcctg cgtaactacc gacggagaag
     4681 aaaccgctga aacaatgcgg tttactctgc cgttctcaca gataatcgac atagacggca
     4741 ttgacgatac tttcaccgca gatgtgagga taacccctgc ggggtgtgat atcataccaa
     4801 agtctgacga ttccggcaca cttgaatgtg agcttgtgct tcttgtgaac tgcgttgcca
     4861 aaaagctttc cacctgtgag atcgtcacag acgcatattc cacctgcttt gagtgcgagg
     4921 ctgaaaggtg tgaaagcaag cttgacagcg aaaacataaa gttatctgac agccacagtg
     4981 ttactgcaaa gctttcctgc caggagggcg agatacgctg tgttcacgac agttgggcga
     5041 ccctttccaa tgtgacatca aggcttgacg aggaaaagaa agcctttatc atttcaggaa
     5101 gcgtgacgtt ctgtattatc ggcaggaacg aggacggcac tcccctttat cttgaaaacg
     5161 attcgccttt tgagcatgag ataccccttc cggagaatgt caatggtgag atatccatat
     5221 cccctgacct gtgcatagaa aactgcacat actatcttgc agacgaaaca acagcggagc
     5281 ttaaagccga tatcaagata ggcggcgaaa tgactattca acagacaggg actatgatat
     5341 cagagcttag ggtactcaca gataagccga aagagaaaaa tgacaaatac gctctgaaaa
     5401 tatgctactg caatgagagc gacgatatct gggagatagc gaaaaagtat tcaacttcca
     5461 taacagccat acttgaagaa aacgagctta ccgacgataa aatatcaaaa cagggaatgt
     5521 tgcttatccc attaatgaac taggaggaca gacacatgaa aaacgatatt tacaccgaca
     5581 ttgccacacg gactggaggc gatatctata taggcgtggt aggtcctgtc cgcacaggaa
     5641 aatccacctt tataaagcag ttcatggagc agttcgttat accgaacatt gaaagcgact
     5701 accgcaagga gcgtgccatt gacgaactgc cccagtcctc cgcaggcaag actatcatga
     5761 ccactgagcc taagttcatt cctgaggaag ctgtgaacat caccctagag ggcggagcaa
     5821 gcttcaacgt gcgaatgata gactgtgtgg gctacataat cccaagcgcc ataggctaca
     5881 tagagaatga agccccgaga atggtggtca caccgtggtt tgacacggaa gtaccattca
     5941 acatggcggc tgaggtggga acgcaaaagg tcataacaga acattccacc ataggtcttg
     6001 tggtgacaac agacggcagt atcaccgacc ttccacgtga agaatacgag gaatgtgagg
     6061 agcgtataat cgacgagctg aagcagatag gcaagccgtt tgtggtgctt atgaacacca
     6121 ctgagcctta ctctcagcag acaaaagacc tttgcgaaca gctttccgac aaatatggca
     6181 ctacagttat gcctataaac tgtttggagc tttcggaaaa agagataaaa gagatactta
     6241 cgcttctgct atactctttc cctgtgaaag agataaacat ttcaatgccc tcatggataa
     6301 actctcttga caagggtcac tggctgaaag aagcggtttt cggacatatc aaggaagcgg
     6361 cttcggctgt gacaaatctt cgtgacattt cagactgtgc cgaaaagata tgctgctgtg
     6421 agcaggtatc atctggttcg gtggctgaga tagaccttgg aaaaggctcg gcagtcataa
     6481 aggtggagct tgaccctgcg ctgtttttca ggataatagg cgaagccaca gggcttgaga
     6541 taaaggacga aaacgacctt atgccgagaa ttttggagct tgtgaagata aaagaacgtt
     6601 tctcgaaatt cagctctgcc cttgatgaaa tggagaaaac aggctacggc atcgttatgc
     6661 ctgaaatgaa cgagctgacc cttgatgagc ctgagataat aaagcagggt ggaaaatacg
     6721 gcgtaaggct caaagccgcc gcaccagcta tccatcttct gaaaaccact atcaacaccg
     6781 aggtcgcacc tatcgtcggc tcggaaaagc aatctgagga gcttgtgctt tatatgctca
     6841 acgattttga ggaatcacct gaaaagatat gggagtcaaa cattttcggc aagtcactcc
     6901 acgagcttgt gagcgagggt ctgcacacaa agctttcaaa gcttccagag gacgcacgca
     6961 tgaagataaa ggaaactatc gagcgcatga taaacgaggg ctgttcggga cttatctgtt
     7021 ttattctcta agagtttcat aaatgaaaat aaactgatcc acaaaaaaag cgttacactg
     7081 tgaaaagtgc aacgcttttt atcttgtata aagtgttagt ttacgccctc tgcaaagctg
     7141 atcggcacac cgaaaagcac acctgcgtta ggctgtgaaa ggtcgccggt aacatcagtt
     7201 atcgcctttc ttgcaacatc tacctgatcg tctgcaacgg caacgaaaat tgtcttgccc
     7261 ttttgtgcag cgtcctcgcc gctgagaata gatctcagaa cgccgatcat aggcagatta
     7321 tccattgtag aaatagattt cgccattccc acgctgtcaa tgaccgttcc tcctcttatg
     7381 cctgcctgtg ccagcgaacg catgatttcg tctactaact cgattctctt gataaccaaa
     7441 aacaataact gcattttgat ccgtcctttc atctcttatt tcctgaatga cgcagccacc
     7501 cagtcttctt tttctcttat ctcaataagc tcaaagccct ctgctttaag cctgtcaagc
     7561 acctcatctt ttctctggtc gattatacct gaaactatga gcgttccgcc gtctttgata
     7621 aatccgccga aaatcccgct catgcctata agaacatctg ccacgatatt tgcacaaagc
     7681 aggtcatagc ctgtgcctat ctgctccacc agagccttat cgtctatgat atttccgcag
     7741 taagctgtat acttttctgc gcttatatgg tttttctctg cattttcctt tgctatctta
     7801 acagaatttt cgtcaacgtc caccgctgtt gcactttcag caccaaggag caatgcgccg
     7861 atagaaagta ttccgcttcc gcacccaagg tcaagtattc tgtcgccctc atggagattt
     7921 ttctccacaa gctccagaca aagctgggtt gtgttatgct gacctgtgcc gaaagacgat
     7981 gcagggtcta tttccagtat ctttctcttt tcgccctcag cgaccttttc ccatgacggc
     8041 tttataagaa gcttttcgcc aacgcaaagg ggcttgaaat actgcttcca gttgttcgcc
     8101 caatcctctt ccttgacgtt tttaagctca tattcaagcc tgccgaactc attgttcacg
     8161 tcacgctttt tgagcgcttt aagctccagc ttcatagctt caagattttc aagaccctga
     8221 gcgttctcag ggagataaac tgtcactgta gtttcacagt tttttagacc cataaggtca
     8281 tcatctatat aatcccagtt gccgtcctta tcatcaagaa agtcctgaaa atcctttgcg
     8341 tccttgataa caaagccgtt tatacccatt ccaagcagac ttccagttac tgcttcgatg
     8401 ccttctgttg tggtatatat atccatttca acccaattca taatatcaat tcctttcgat
     8461 aatatctcat tataacacat ttttgcgatt ttgtaaagct ttttgactat gatttattat
     8521 acaaaattaa atattgataa ttgtatatgc ttatcaaata tcaactatag taggtgcaaa
     8581 caaaaagctt cccgtcaaaa aacgaaaagc tttcatgatc attttttctt atcgtaagcg
     8641 tcaaaggctt cctcaacagt gccgtccacc tgcaccttgc cgtcttttag gaatatcgcc
     8701 cttgtgcaag tttcctcgat agccttgcgt gagtgggaaa cgaacagtac cgttacacca
     8761 gtggcgataa catcatttat cttgtcacgg cacttcttct gaaagtctgc gtcgccaacg
     8821 ctcagtgcct cgtcaacaac gaggatatca ggcttgatat ttatattgat agcaaagcca
     8881 agacgcattt tcattccgct tgaatatgtt cttacaggtt ggtcgatata ctctccaaga
     8941 tctgcaaaat caattatatc ctgctctatc tcctttatct gatcgtcatt aagaccaagc
     9001 agatagccct ttagatagat attttccata ccagtcatat ccagtgaaaa acctgctgta
     9061 agctcaagca atgcggcaac ctttcctctt acagtgatag tgccgctgtt tggaaagctt
     9121 acgcctgtta tcattttcag aatggtcgat tttcctgcac cgttctttcc gataattccc
     9181 acagactcgc ctgggtatat cttgaagctc acgcctttaa gggcagggtg tttctttact
     9241 cccctgtttt ttgtgaaaat agccttaaaa cgtgcctttt cgtttttata taaaaagtat
     9301 tcttttctta cgtccaaaaa ttcaataatt ggctcagtcg cttgattttc catttaaaag
     9361 tcagttcctt tcagcctttg ttacagaacg tcaggaagta tcttcctgaa cttcttatag
     9421 gtgtgcgctc cgaggaatat cataaagata aactccgcat agaacaccat agtttcggtg
     9481 gtatagttag aatcaaaaat aaacgtgtct gtaagaaagc tctttctata gccgtttaca
     9541 aaatagttta tagggttgaa atacatgagc tttctcagcc acaaaggtga gatatcatat
     9601 gtgttccaga atatacctga aagccagaaa agtcctgtta taacagagtt tataagattt
     9661 tcaaaatcct tgctgaacgc actgagagga gcagtcatcc acgaaagcac aaggaagaaa
     9721 aaatacatca tcagcgcata atagataaac tgaagattgt aaatactcgg tccaaagccc
     9781 aaacacagca acaccacata cattatagca cacagcaaaa tgtggatata aagtcccgaa
     9841 agtgaggtaa aggtcatgat atttgaaaca gggaaggaca gctttgttac aaactgcttg
     9901 ttgcttctta ttgaccttga tcctccaacg atacattctg aaatataaaa ccacggcaca
     9961 tagccagtga gcatgaaaac gaactgagta tactttccgt taatgaaatg cactccaccg
    10021 tttcttttta gacctatctc aaaggcaaac cacagtataa acagggtaaa ggtcggcttg
    10081 ataagtgccc aaatcggacc taacgctgcg cccttatact tctttttcag ttcgttcacc
    10141 gacaacattc ttatctgtct gagctttccc ttatgctcct tcaatatctg ggcaagcccg
    10201 aaccaatcaa acttttttcc ttctattttt atcatctccg tcaaaaacgc tctttttcca
    10261 aagcgctgtc gattattctt tccgttgacc tgccgtcgca ggcagacata tatttttccg
    10321 tgaaacgctc cacgccctca tggtcaaagc tcttttcact gtcaagcaca gcctgtgcaa
    10381 gcttctctcc ctccgtcacc aaaggcgcag gcatatctgt tttataatca agataaaagc
    10441 tcctgccctt gctgtagctc tcaatatcag gggcaaatat cacccacggc tttttataca
    10501 gcgcatactc gaacacaagc gacgaatagt ccgtcacaag cacgtctgcc acaggcatga
    10561 gcctgtttgt agcaatatcg cagttagtca gcccgctgtg aagtctgggg tgcattttta
    10621 taatgacttt ccaagcctcc ccaagctttt tttcaagctc tgcggcatac tcctcgccca
    10681 cgcaatgaca atccagagca ttccccctga acgtcggcgc ataaagcact atcttcttgc
    10741 ccttatagtc gggatatctc tcaaagaact cacgtctgca ctgctcgttg tagctttcat
    10801 caaagaacac gtccgtacgg ctgacgccaa ggggctttac gatatcttct ttcagtctga
    10861 acgcagacac gaaagccttt acgcactctt tactgctcac agtgataagg tcaaaatttc
    10921 ttgtaacaga gcctttgaaa tgggaagata tatcctcctc gctgtcatag ccgaactttt
    10981 tcaatgctcc gcaggaatgc caaagctgaa ccactgtggt ttcctcacgc tttttgcagg
    11041 aagtcacagg cacaaaatag ttgcatatga acacagccat tgcatttgcg taagctttca
    11101 taaagctcat catatattta agccacgaga ccattccaca gcttgaaagg tcacagtaca
    11161 tctccctgac cttatagcca cggctttgaa gctccgcttt tataagctcc atgctctcag
    11221 gagtagaaac gctgttaagg tctgcaagga taacgagctt tttgtctata tcctttttct
    11281 ttgacagctt gtagcacagc ggcaaaagcg ttttttgcag tgctgatttt atgtgctgtc
    11341 ttattatacg tttgcggctc tccattatcc cctaagaaac ctcttatata tcttgcctgt
    11401 cagcgatttc ggcattatga aaatgaaaaa ctgaaaagtg catggaacaa taaagtccca
    11461 cagatttgaa tatcccatac ggaatatctt ccacctgaca ttcacaaagg actttgtgtt
    11521 cgcccagttt cttcttctct cataattgtt cgccgtaact ctgtacatca caagcgtttt
    11581 gtcaagattt attccccttg cgccgtccgc aagcattttc accacaaact catagtcctc
    11641 acagcgtttt atattgctgt aacctcccac gctcaaagca cgggatttct tataaacaag
    11701 ggtctgatta ttgaacggat ttctccgcct tgcatatttg tgtatttctt tattggtcaa
    11761 tggcgttttc tttgtggcta tcacttcgcc cgtctgactg tcgaactcgt ctatataagc
    11821 cccaagcata tctatctcag ggtgcttcgc caaagcataa agcgaccttt cacagcggtc
    11881 aggcttagcg atatcgtcag agtccatttt gactatatac tcatttttgc aggcgtctat
    11941 acctctgtta gcgcatcttc ctgtgccaac attctccttg agccttatgg ggtgaaagca
    12001 ctcgtaattc tcctcatagt agtctactac ctcgtcaagc tcttttgtga gctttccatc
    12061 gcacacaagc acaaaatcgt tcgtcacata ggtctgtgac agcatactct cgatacttgc
    12121 cctgagatgt tcaggcttct ctcctgcata aacagacata agcacgctgt attcaggcaa
    12181 ctccacattt ttcactacca tagcgcaccg tccttttttc attctgtcaa aattatcccc
    12241 acaaatctgt aagctattcc attataccat taaaaccgct gtttgtcaac gcaggcgggg
    12301 cttaactttt cccaaaatca acaataaaat gttgaaaaaa atagcgcatt taagcaacaa
    12361 aaggcaaata aaaaaatgaa agaaggagcg tattgcacta tgatcgatta tagcacactt
    12421 aatagtaatt tgaaaagagg cattttatca aaaataagtt atagccttaa cgatcgcaat
    12481 aaatactatg gatatttcat aggtcataac gagaaatgct attgtcaata tagctttata
    12541 catttctctc aaatattccc aacttttaca cccaaaatga gcaaacaacg caaatgcgca
    12601 gtcccaactt ttacacaaaa aaggcggcat tttactgccg tcctttgtaa caagacctta
    12661 tccgtcactt tatgacctta gcgcatatct tttcgtaatt gtctatctta tagttgagtc
    12721 tgtcgatagt cgcagctatc tcagtctgct tttcaaggag atcttttctc tgctcgataa
    12781 gcagattttt tcttgcctcg atagtgctgt cgccctgctg atacaaagca acatactcga
    12841 taagggcttc tatttgcaga cctgcgcctc tcatgcactt tatgaactct atccaattgc
    12901 atgagctttc atcatagtcc ctaagaccgc ttctcgttct cggcacaggc ggaataagtc
    12961 ctatacgctc atagtatctc agtgtatcgg gtgtaagccc atatttctca ctaacttcag
    13021 aaatagtcat agctttacct ctctattaaa gtgcattata ctgctcgtct gaaacaggct
    13081 cgcaccactc gttcgatgcg ccctcggcag gtatctcaag agcaacgtgc gtgaaccact
    13141 cgtcctttgt tgcaccgtgc caatgcttta tctccggagc aatgtaaaca acatcaccag
    13201 ggtgtagctt tcttgcaggc tgaccccatt cctgatacca gccctcgcca tctgtacaaa
    13261 gaattatctg tccgcccttg tggtggatat gccagttatt tctgcacttt ggctcaaagg
    13321 taacgttaaa aacgcctacg cccttgacat tcagcggttt aagatagctg ttacctataa
    13381 aatactgagc aaagttcaca tttggctcgc caagaccaaa aagcgagtct gaacagccgt
    13441 ccttttcctc agcatacgct tcccttacca ttggaaagac agcccaagcg ttaggccagc
    13501 caacatagaa tgcagcgtga gtgatgatct ccaccatttc agtctttgtt actccgtgat
    13561 ttttagcgtt ctcgatatga tatttgatag aattgtctgt aatgcccttt gccataagag
    13621 ctgttaccgt aacgatacaa cggtctctca gtgagagctt gtcctctctt gaccatacct
    13681 ctccgaaaag aacgtcatca ttaagctctg cgaatttagg agcaaggtcg ccaagactat
    13741 ttctgcctgc tgtctgtttt actgccatgg taattacctc ctggagtttt atttattgat
    13801 tacattttac cacctggagt tagctccaag tcaagacctt ttctataaaa tcgtcgatat
    13861 gcacaaatcg tcatgcaaaa tttgttatca ccgacaatag cttaacgata gtccgacacc
    13921 ttaagaaaat cttatgaaac agttaagcta atactcgtgc gaaagcttct ttaatatggt
    13981 atcattgata catcaaaagc aaaggagcgg tcattatgac aaagaaaaac aaaaagcata
    14041 tcgtaatcgc cactgcaatc attattttgg ttgtggcatt gcttatatac agatattaca
    14101 ttcctgatat gaagcccaag aagcagttca ctcctgcatc agtggaatac acctatgacg
    14161 acagcgtgac ttcatctcag cttgcggagt ataatgcccg gcaggactgg cagctcacat
    14221 tggtgaacaa cgacacgcct atatgtgaga attgctcagt gatactcacc gcccttgaca
    14281 atggtcatgc agtggactgc cgcatttacc cacagcttca gaaaatgttt gatgacgcgc
    14341 gctcgcaggg gatatatcca actatcacat catcgttccg caccgcagag gatcaacagg
    14401 acatacttga gagcaaatat gagcactatc agaaaatagg ctacagcgac aaaaaggcga
    14461 aagcctatgc ggaagaatgg gtggcgatgc ccggcacaag cgaacacgag cttggtctgt
    14521 gcgtcgatat cgcaagcggt gacagcagag ttacaaatca ggaagtatgg gattggctcg
    14581 ccaaaaacag ctacaaatat ggctttatag agcgttacac agccgaaaag caggatatta
    14641 ccggcattat cccagaagaa tggcactaca gatatgttgg cgaaaaggcg gcaaaggaaa
    14701 tgtataaaaa tgacctctgt cttgaagaat atttacagaa ggtgtcataa aaaatgcggg
    14761 cggccaacgt ttgaccctac gcaaaacagc gtatacacat catttgtagg ggctggcgtc
    14821 ctcgacagct cacttatcaa agttttttac attcacagca ctgtatcacc caccgataca
    14881 gtgcttttat tttgcaaact gatttttgag tatcccaagc atatcctttg cctgctgttc
    14941 aagctgcgac ctgagataat agacaccgaa agatatatct ttcacgcctt ttatcggtat
    15001 cgcttttagc ggacctttgt aagcgtcccc tattgcgggg aaaatcataa accccagccc
    15061 cgcttccgcc attgcaactg cgctttcata atctccacag aatattatct tatccacctt
    15121 gccgttcgca agtatcctgt gctgcaaggc aaatatctcg tcaggacagg tgtggcgctc
    15181 tataaaaata ggtctgcctg agatatcgtc cacatcaagg ctagccgcct ttgcaagggg
    15241 gtgatctttc tggcaaagac acataaactg acagcggcaa agctccacat agtcaaaagg
    15301 ctgtttatga ggacttttaa atgacaaaag aagctgtatc ttctgatctt tgagctgatt
    15361 gtctatggac ttgaaggggg tgagatctat atacggcaaa agatcatcat aaagcctgga
    15421 catttctctt agggcaggag tcaaaacgtt cagctccgcc tgattgtgac agcctatgct
    15481 tatccttaca ggttcgtcct caggcttttc tgacagcctg aattttgctg agcctgctat
    15541 tttaagtatc tgctctgcgt ctgaaataaa cattattccc tctcttgtga gcgaaacgct
    15601 cttgctcgtt cgcacgaaaa gcttcacccc tagctcgtcc tctaaagaac ctatctggtg
    15661 gctcaccgca ggctgagtga tgctcacaga ttccgccgcc ttggaaaagt taagatactg
    15721 cgccacagcc aaaaagcact ccaattgtac agtattcaca ataaccgccc ctttatgttt
    15781 gtttatttcc gcaaattgtt tcgtttggta aacaattctt aataaaaatt aaatttgata
    15841 gcaatctata aaattctttt atnnnnnnnn nntatttgta tttcactttc aagtaaaagt
    15901 gtgatattat taattaaagc aaaatgttag gtacaaaaat gtaaggaggc gaacaatttg
    15961 gcggataaca gaaaaaagaa cattctgcgg tattccaaat acattcatct gaaaatagaa
    16021 tgcgagataa acaccatgct cgcaggcaag ggtataaccg ccgctcagag ccatgtgctt
    16081 atgtacataa tggaacataa tggcgagcct gttttctcaa cggcgataca ccacgatctg
    16141 aatatttccc gtgcgacggt atcagggctt ataaaaaagc tccgtgcagg aggatatctc
    16201 tcctatgagg gcagtgatga tgacgagcgt cacaagaaga taatagcaac cgagaaagcc
    16261 tataccctcc agcaggacat ttgcgactgt cttaaaaaga tagaaacaat ggctttccgt
    16321 aattttacag atcaggagct tgacgaaatg gaacggctcc agaaaaaaat gatagacaat
    16381 acaatataaa aataagcgag aggagggatc tggcttgaaa gaaatactag cacagatcaa
    16441 acagtacaag aaggactcca ttctgacccc tgtctttacc gcacttgagg tctttatgga
    16501 aatgcttctg ccttttataa tggcgaaaat aatcgacgac ggcattgaaa agggcaacat
    16561 gacaaacgtt tatattttcg gcggcgtaat ggtagtggca gcaatggtca gcctgctttc
    16621 gggctttctt gcaggaaaat atgcggcttc ggcttcatca ggctttgcct gcaatcttcg
    16681 tgaagctatc taccgcaagg tgcagactta ttcgttctca aacatcgaca agttcagcac
    16741 cgcaggtctt gtaacaagaa tgacaactga cgttacaaac gttcagaacg cataccaaat
    16801 gataatccgt atcgcagtga gagcaccgct gatgctcatc tgctcaatgg caatgtgctt
    16861 tgcagtaagc ggctcaatga gcactatatt ccttgcagct atcgttcttc ttggcatagt
    16921 tcttggaatc atcgttaaaa agacgatgaa agttttcaca gaggctttca aaaagtatga
    16981 cgaccttaac gcaagcatac aggaaaacgt atcggctatc agagttgtaa aggcatatgt
    17041 aagagaggac ttcgagcaga agaaattcaa gaaagcttcg ggcaacctct ataagatgtt
    17101 cgtaaaggct gagagcctta tcgcactgaa cagccctgtt atgatgttcg ttatctactc
    17161 ctgcataatg ctcataagct ggcttggcgc gaagaatgtt gttgcaggca cgatgacaac
    17221 aggcgagctt tcatctgttt ttacatatgt tatctccgcc atgatgtcac ttatgatgct
    17281 ctccatgata ttcgttatga tatcaatgag tacagcaagt atcagacgta tctcagagat
    17341 actcaaggaa acacctgatc ttcacaacgg tgagaaccca gccaaggata tgaaagacgg
    17401 ctctgttgac ttcaacaatg taagcttctc atacaagcat ggaagcggaa aaatggtgct
    17461 taacaatatc gacctgcaca tcaggtccgg cgaaactatc ggcatcatag gctcaacagg
    17521 ctgcggaaaa acaagccttg taaatcttat cagccgtctt tatgacgttg acgaaggttc
    17581 tgtatgcgtt ggcggcaagg acgtaagaga ttacgatatg gaatatctga gagatcaggt
    17641 ttctgtagta ttgcagaaga acgtgctttt ctcaggcact atccttgaca acctacgctg
    17701 gggcaacgaa aacgcaacag acgctgagtg tatcgaagcc tgtgaagctg cctgcgctga
    17761 cgagttcatt gaacgctttc ctgaaaagta tgaaacttat atcgaacagg gcggtacaaa
    17821 cgtatcaggc ggacagaagc agcgactttg tatcgcaaga gcgcttctga aaaagccaaa
    17881 ggttcttatc cttgacgact ccacaagcgc agtagacact gcaacagacg caaagataag
    17941 agcggcattc gcaaagtcta tcccaggcac aacaaaattc attatcgcac agagaatttc
    18001 aagcgtccag gacgccgaca ggataatagt tcttgacggt ggcagagtaa acggctttga
    18061 cacacacgaa aagcttgttg aaacaaacga gatataccgt gagatatatg aatctcaggt
    18121 aaagggcggc ggagatttcg acgttcagtc agcaggaaag gaggaagctt aaatgcctaa
    18181 gccaatgaat aaagaaatgg gcggcaagcc taaaatgtca ggcgaccaga tgaaagtcct
    18241 cggcagagta atgaaatata tgctcaaaaa ctataagttc tcatttataa tagttgtggc
    18301 gtgcatactt attcaggctg tcaccacact tgcgggaatg atgttcatgc agtcacttgt
    18361 tgatgactac atcacaccga tgctaaagac ctccagccat gatttttcac ctcttgcaaa
    18421 ggctctcggc ggtcttgcag tagtatacgt tgtgggcatt gcggcttcat acgcatacaa
    18481 cagaataatg gtaaacatcg gtcagggtac gctgagaaac ataagagatg atctgtttct
    18541 caacatggaa acacttccta tcaagtattt tgacacacac ccgcatggcg acatcatgtc
    18601 agtatacaca aatgacgttg atactctcag acagtttatc agccagagca tacctcagct
    18661 tataaactct cttgcaacac ttatatccac cctcgtttcc atgataatcc tcaacattcc
    18721 tcttaccata ctctctatcg caatggcatt tgtaatggta aaggtgacca acgatctcgc
    18781 ttcaaagtca ggagcgcact ttgcggctca gcagcgtgac cttggcgcag ttgacggttt
    18841 tatcgaagaa atgcttgacg gacagaaagt agtaaaagtg ttctgtcacg aggataaggc
    18901 aatagaagac tttgtaaaga taaatgacaa gctgagaatc agtgcaaacg aagctaacaa
    18961 gtacgctaac atcatgatgc cgataaacgc aaatatcgga aacctcagct atgtgctttg
    19021 tgccattgtg ggagctatcc ttgccataaa cggctatgca ggtcttaccc tcggcactat
    19081 cgtaacattt gtatccctta acaagaactt cacacagcct atcacacaga taagccagca
    19141 aatggcattc gttgttcagg ctaccgcagg cgcaggcaga gtgtttgacc tcctcgacca
    19201 gaagcctgaa acagacgagg gctatgttga gttcgttaat gcaaaatata acctgaacgg
    19261 tgagcttgaa gaatgcaaag agcgtacagg catatgggct tggaagcatc gtcatcaggc
    19321 tgacggcact gttgactaca agaaaatgga gggcgaggtc gtatttgacg gtgttgactt
    19381 cggatatgac gacagcaaga tcgttcttca tgacgtaaag ctttttgcaa agcctggtca
    19441 gaagatagct tttgttggct caacaggcgc aggaaagaca actatcacaa atcttatcaa
    19501 ccgcttttat gacattcagg acggcaagat acgctatgac ggaataaaca ttacaaagat
    19561 aaagaagcct gctctgagaa aaactctcgg catagttttg caggacacac accttttcac
    19621 aggcacagta atggaaaata tccgctacgg caaccttgac gccaccgacg aggaatgtat
    19681 ggcagccgca agacttgcaa acgcaaacgg ctttatagaa agacttcctg acggctacaa
    19741 cactgttctc acaggcgacg gctcgaacct ctcacagggt cagcgtcagc tcatagccat
    19801 tgcaagagcg gcagtcggcg atcctcctgt tcttatcctt gacgaggcta cctcctctat
    19861 cgacacaaga acagagagtc ttgttcaggc tggtatggac gctctcatgc agggcagaac
    19921 aacattcgtt atcgcccaca gactttcaac tgtcagaaac tcagactgca taatggtact
    19981 tgagcagggc aggataatcg agcgtggcac acatgacgag cttattgcca aaaagggcaa
    20041 gtattatcag ctctacacag gaaacttcgc agaagaaaca gcataaaagg gctattccgc
    20101 ccaaagaaaa acgccacgga gagaatatct ccgtggcgtt tttgtgcaat aaaaatgcgg
    20161 ctgacaaaag ccaaccgcaa taaatcttat tcttcttcct cttcgtcctc atcgaaatct
    20221 tcttcatcga tgcccattgg acgaggcata tcatctgtca taggctcatc tgctcttctg
    20281 aaatatgagt cgaaatcaaa acgtcccatg cagatacgct cccagtatgg tttacccggc
    20341 tcaagcacca taacattatg ctcatgaact tcgataacat catagatctt gccgtatgaa
    20401 tcctttacct gatcgccctt tacaacattg gtcattatat tcaccttcct tgtcatatct
    20461 tgaattatac cacattataa gagccttgtc aatcggcttg gaaaattttt tgccctaaat
    20521 acttaccttc ttcacagccg accacttgcc gtacactttg gtcttgccaa ttgtcttata
    20581 atcccttgcc ttgacgtaat atgtcttttt gcttttaaga ccttttattg tgcctttgtt
    20641 ctttgagttt ccgctgagcc acaaggactt gcttttctta aagccgctgt tgtcggcata
    20701 tgacacttga tagcctgaat ttgtgctgtg ctttgcaaga gtgaccgcta tcttctttcc
    20761 gctcttgtcg cccttggcag aaatgctctg tccctgtggg tttatcttga aagtctttgt
    20821 tatcttacct gtgtagcttc ccttccctgt gacagtcacc ttagcagtgc ctatgccctt
    20881 gtttgcggag tagcttacag tatagtccct gtcctttttg agggtgacat ttccgtcctt
    20941 tacagtgaca gcaggcttca gcgccttacc tgtccacacc tgatctgaca ccttgacaga
    21001 cgccttcccg acagatcttt tcgttgctga tcttaccttt atctcagcgg aatatccacc
    21061 gtataccttc ttacctgaca cagtttcata acagcgtact ttgtaataat aatctgtgtt
    21121 tgccttgaaa aatccatctg taaactttcc ttttgcagtc gtccccacgg cagagtattt
    21181 tccctttgca cctgtttttc tgtatatctc atagccgcat ttgttagttg atctgccaga
    21241 aaagctgatc tgtatatccg tgttattcac agaattaaca gacaatcctg atactctcgg
    21301 caggctgata gaagaatcag cgctctttga cactgccgca gactttgagc cgttcacagc
    21361 gttgtctttc agcgaagcct tgctgtgaga gcttacgctt atgccgcttt ttactgagcc
    21421 acttatactg ttggaattta cactcacaga actgctgtaa tttgacacgc aaatgccgtt
    21481 ctgcttgctg tttgatatct tatttgagct tagaaccaca ctgccgcagt ggtctgtaac
    21541 aagcacgcca tgcttgcctg cgccagaaac agtgtttgaa accgccttgc ccgaagcgtt
    21601 cttgctgaac acgataccat gccccacggc atttttaact ttgttgcctg agcactcgcc
    21661 ctttgaaccg ttataaataa gaattcccgt gtcccccgcc tttgaaatgc tgttggactt
    21721 tatcacagcg gaagaatttg cagccactgc tatgccatgc tttttcgctg aatttacgct
    21781 gtttgagctt gcaatattgt tctcagagcc gttgtagata tttattccgt tcattccaac
    21841 gttctctata gtattgttct caagggtatt ccctgagctg tcagtaacag aagcaccgtt
    21901 tctggctgag cctgaaatat agttatctgt tatctttata ttatcacact cgttgagagt
    21961 aagaccattt ttatccttgt aattaacacc gtcctgcttt gtaacgagaa tatttgattc
    22021 gatactgcaa ttttcagcgt cattaagtgc gatagtggtt ccgttgctct cgattcgatt
    22081 ttcgaggatc tttactcctg ccactctgaa attatagtca gggataacac cgttgtcctt
    22141 gacctcatac cctgtcaggc gtatgccgct tgtaatgcca tatttgtcat caccgcttac
    22201 agagatagtg ttagatgaaa taacagagtt tgcgtcgtcc tcaaagccgt caactgcttc
    22261 ctccatagac tttaccacag ggggattgaa attgttgtta ggcaggctgg acattgcctt
    22321 gaactctata ccgcttgcgc agtttgtgat agtgttattt gttatggtac aattcttata
    22381 attgtacatt atcattgcca catcgtcgag gttctcaaaa acgttgccct caataaggat
    22441 atctgtataa tagataccca gagtggcgga atgagaacca agacctctgc aaatgcctga
    22501 gaaaagatta ttctttatga ccacattttc gcatgatgta tcgtcaaaag gcgcatagcc
    22561 tgcaaaaact ctgctgttgt tcatgcaatc aagctgaatg gcttcttttt tcctgtagcc
    22621 cgtatatcct gtaaaagtac agccctctat ggtgacgtca gccgcaccgc ctatctccat
    22681 atgatgaccg ttctcgttat ttttgacgtg catatctttc agaagaatat ttctgcaatg
    22741 tgcaaatctg atatttgaaa aatctgcggt atttggccac tggtctgtgt ttccgttcca
    22801 aagcccgccc tcaacgataa catttttaac gccgtcatag ccgccgatac cattattatc
    22861 gcacctgagc ataggtccat aggaaatgca cctttttatc tcaacgccct cttcacagta
    22921 tatccaagta tcgctgtata ccacaagtgc ataagaaatt agatacgttc cctttggtat
    22981 cacgaccttt gtgaaaatgc cctgttctga attttcctgt gcagcgtcaa gtgcctgctg
    23041 tatctgcttt ccggtcatgg cgtttcctgt attgtctgca ccgaagtcat taaccacgtt
    23101 ataagtgacg atttccatgc cgtctgtatc aaaatacgca cctgtcacag ccgtatctgc
    23161 cacttctgcc actgatgtta tagccaccga gaagccctcg ggaatcgctg tgataacgag
    23221 caagagcgtt gtcaaaagtg ccgctatccg tctgaaaacg tttttttcac tgataattct
    23281 gtagccttta atgttcattc tgctgtgttc tccttaaagt tcggctatat ttttcttgcc
    23341 gctgacaatt attcgtacaa atagtattat aaataattta taagtatatg tcaacaaatg
    23401 ctaaactttt cgtgaacact gcacaaattt ggctgtgtat ttttggcaca aaaaaagctc
    23461 ccctgacgca taaacgtcaa gagagccttt aaagcaaaat attttactgc acttccactt
    23521 tttcgcccat gatagacctg atagcgtgga cacggcacac ctctgcacac tttccgcagt
    23581 ttgtacactt gtcatagtcg ataacagcgt ggttgttctg cactgtaacg gcacctgacg
    23641 gacagttttt ctcgcacagc ttacatccta tacagccgtt aggacaaaca gatcttgtca
    23701 ctctgcccac atctcttgat gaacagcaga tatccacagc ctgagagatc cttctcactg
    23761 cgataagacc atttggacag gcttttgcgc aaagcccaca gcccatgcac tttgccttgt
    23821 cgataacggc aatgccgtca acgatagata ttgccccctg agagcagacc ttgatacaat
    23881 cgccgtagcc taaacagccg cttgtgcaaa gcttgcttcc gtcataaaat ctgttgcacg
    23941 ccgcacagct ctgaatgccg tcaaaatcgt atttatgcgg tgtatgagag caatctcccg
    24001 aacagcgcac gaaagccacc tgtggctctg ttacagtcac ttcaacgccc attatctcgg
    24061 ctatcttctt tgcagcagcc tcgcctccag gacggcaaag gttacattcc gcacccttgt
    24121 tcacgatagc gtcggcatag tctgcacagc ctgaaaagcc acaagcacca cagtttgcct
    24181 gcggaagagc ttctgcgata gcgtcaacac gctcatccac ctcaacggca aaaacttttg
    24241 aagccaccgt cagcagaacg cctgccaata tgcccaaagc ggcaaatata gccaaaggga
    24301 tcaaatattc catatcattc tcctctttca gaatttcagt ttttgttgtg tttcattata
    24361 ttattatcca taaaatcaag ggtcatctta atgcggtttt tgatattaac gtccgctgtt
    24421 cccgaatcgt gatcgaccgc cgttatgatg ctgtctgggt agagcttttt tatacgcttt
    24481 attatgcctc ttgcacaggt gtggctcaca aggcagccaa acggcaacag catcacaaca
    24541 cctttgcagc cgttcttcaa cgctgaacac atttctgcac cgataagcca gccgtccccc
    24601 attgtttcgc caaggcagcc aaagttttca ccgtagcttt tcaaggatct atagtcttcc
    24661 ataggctcga aaccgttttg tacaagtgca gaattcattt tgcaccaaag cgttttcata
    24721 taggcaataa ggacttttgc tcccacacct gccaaggttt ttcttccgta aatgttatca
    24781 tcatttgtac aggaatcggc aaggtacatt atgtatggaa caaagccgct catatatatc
    24841 tgacaaccga gatcacggag gtatttctcc gtttcgccgt tgccgagagc acagaatttt
    24901 atgtaaagct cccctgttac ggcgaccttt ttgaccgatc tgcttttatc acaatttatt
    24961 gtagcaaagc tttcgcatat cttgcggaat atgacgcaca ttgagatcgg atcggctgct
    25021 ttgccatgtt caaagagacg tgacagcctg ctgttccata ggcggacttt atcaagcgtt
    25081 tcaccctttt ttagttcata gggtgaaagc tcattataaa gatacaaaag caagtcactg
    25141 taatagatcg ctgcacaagc cgtcagcacc attttaggcg ttatggaaaa tccgctgtgt
    25201 ttctcaacgc cgctgacatt aagtgacacc acaggaacat tttgcagacc ggctttcaaa
    25261 agagcttttc gcagcagcat aatgtaattg cacgctctgc accctcctcc tgtttgaggc
    25321 agcagcaggg ctgtttttgc ggtgtcatat ttgccgcttt tcaatgcaca tatgaactgt
    25381 cctattatga ggttgcaggg aaagcagaca tcattatttg aatattccca gcctgcgttt
    25441 atcatttgtg accgactgta ttcgttcacc ccgataacgt cgcacttata tccgccgcta
    25501 agaaatgcat atttaagcag cggaaagtgg aagtcaagca ttgatggtat caatatagtg
    25561 tgggtctttt tcatcggctt tgtaaattca acccttgatc gtttcatatc gataaattat
    25621 tatcagatta ttcccgaaaa gcccataaag cttagcgaaa cgatagacgc cgcaatgagc
    25681 gttgcaggca cgcccttgaa agtttcagga aaatcgcagt tttctatcct tgctcttact
    25741 ccgctgaaaa gcaagagggc aagagtaaac gcaagtcctg ttccaaatgc atttgtaagg
    25801 gactgaagaa aattatagcc gttgtctata tttccgatag taacaccgag aactgcacag
    25861 ttggttgtga taagaggcag ataaacgccc agtgccttat aaagaggcgg catcatcttt
    25921 ttcatgacta tctcaacaag ctgaacgaac attgctataa caagtatgaa tgccactgtt
    25981 tttagatact ctataccgaa atgctcaaga agtctgtata caggatatgt gatagctgtt
    26041 gcgcatatca taacgaatat taccgctgcg cccattccca cagatgattt catctgcttt
    26101 gatacgccaa ggaaaggaca tattcccatg aactgagaaa gcacaacgtt atttacgaac
    26161 acacagccga taagtataag tatgtagctc atttttatgc ctcctcactc tctgttctgc
    26221 actgaccctt gcacaaagcc gcatttgggc agcctgagca ggatatctcc tttggcggct
    26281 tcttgttcgc tatcttgttc acaagtgcga taagacagcc aagcacgaaa aatccgcctg
    26341 ccggcatgat gaacactgtc ataggatcgt cgcacaggaa aggtatcttg aagccgaacc
    26401 atgagcctgt tccaagtatc tctcttatag atcccattgt gaaaagcgaa agtgtaaatc
    26461 caagacccat tccaagtccg tcaagtattg acgggaatat cttgttcttg ctggcaaaag
    26521 cctccgctct gccaaggata atacagttta ccgtgataag ggacaggaac acgcctaatg
    26581 cactgtaaag ctcaggtata tagcctttca taagaaactc gataagagta acgaagcctg
    26641 cgattattac gatgaaacaa ggcagtctta cagcctttgg gataacgttt ttcaaaaggg
    26701 atataacaaa gtttgagcag acaagcacga acgttgtggc aagtcccatt ccgataccgt
    26761 ttttcgccat tgttgtgacc gcaagcgtcg gacacatacc aagaagcgta acaagcgtcg
    26821 ggttttgctt tatgataccc tttgtaaatt catacagatt agaatttttc tcagccattg
    26881 agtatctcct ccttatgctc gtcaaaaagc ttcacagcct ttttgcacgc agatttcatt
    26941 ccctttgaag aataggtggc agcagtaaca ttgtcgatag aattttcatc agtgtcaaca
    27001 caaaagccct ttagctttga aagaaaacta tcgtcctgca cctttgtgcc aagacccgga
    27061 gtttcgccta tagagagaaa ctctatccct tcgacagttc cctcgctgtt tataccgata
    27121 agcagatgaa gtccgccctt tgaatagccg tcctcagtta tctcgataac gcatctgccg
    27181 ttatctttat ctgtgataat ggagttcacg ccctctgtat caaatgtgac aggggtcttg
    27241 ctgtcaccct cgccgtcaag cattatctca taatcgccat tgccgaatat ctcctcacag
    27301 ccctttttca tatcgtctgt aagaacacct gtattgtcaa catatgtagc attataggca
    27361 agcaccaaaa gtccgctgac aactatacat attatagtca gcaccaatgg aggcattatt
    27421 ttatctttca ttcagacttc gcctccttct tctttgttat agcccctaaa ggcttagttt
    27481 ttgttatcat gtcgatatat ggtgtaagta tattcataag cagtattgag aatgaaacac
    27541 cctcaggata agagccgaag aagcgtatca cacaagtgat gataccacag ccgataccga
    27601 atatcacctt gcctgttttt gtaacaggag ttgtaacata gtcagttgcc atgaagaatg
    27661 caccgagcat tacgccgcct gcaagtatct gatagatacc gtcctcgccg caaagatatg
    27721 agaacacgaa aactgtgcca ataaatgcaa gaggtgtatg tactgagatt atcttcatca
    27781 ttataaggta tatgccgcca atgagcaaag ccgccgcaga cacctcgcca agacaaccgc
    27841 ctacttttcc aaggaaaaga tcgagataat cggcaggggc ggaatgtgtc gccgcaagag
    27901 gtgttgcacc tgttacgata ttgtcagggt catagaaagg cactacccaa tgggtcatgt
    27961 tgcctgtgaa agcaagcata agaactattc ttcccacgat agcagggttg gcaaaattct
    28021 gtcccaggcc gccgaaaagc tgctttacga ttacgatagc aacgaatgtg ccgatagcag
    28081 cctgccaata aggcattgtc acagggagat tgaacgctaa aagcataccg gtcacaagtg
    28141 ccgaaacgtc ctttgtggaa tcaggtcttt tcattataag attgcaaagc ctttcccata
    28201 tgagtgccgt tatcatgcag aagcctgtaa gcatgagcgc acgtccgccg aaaacgtagg
    28261 cagacattgc aagcgcaggg caaagcgcca caagcacatg ggtcatcacc ttagaggtag
    28321 aaatattatc ccttatctga ggtgaagggc tgacaagcat tttttccata ttttcttacc
    28381 tccttacgtt cttatgaaag cttttgcaag ctgatttttc tctgcaagat ctctctttgc
    28441 aggacagacg tatgaacacg aaccgcagtt catgcaaaga cccaccttga gcttcataag
    28501 agcttcctta tctcgcctgt cataagcctt ttcaagctct gtaggcataa ggttcagcgg
    28561 acaagcagag atacatcttc cgcactttat gcagtttgtc tgtgtgcatc tgtcctcgat
    28621 accctttttc ttatcagcct tttcaccaaa tgcaagaatg gcgtttgtgg tctttgtcac
    28681 aggggtgtca agctcataaa ggcacattcc catcatagga ccgccaaaaa gggcttttct
    28741 aaccttttct ttatcacagt caccgaattt cagtatatcc tccgcagaag ttcctatagg
    28801 tactttcaca ttgcatggag tagataccac gtcaccgtca acagttatac gctttgatat
    28861 gagtggcata cctgttttgg aatactcacc gataaagcct gctgtggtaa cgttcattac
    28921 gataacgccc tgatctgacg gaagctgacc ctcgccaact acacgtcccg tggcgttgaa
    28981 tattatcacc ttttctgcac cctgaggata gcatgacttc agcaccttta cttcgatact
    29041 ttcatcatca gccgactttt cacgcataag ctttatagcc tgtggcttgt tgtcctcgat
    29101 accgataacg acctttttaa gccccagcat atcacgcacg agctttattc cgcacataac
    29161 atcgtcagcg ttctccatca tctcacggta gtcgcttgtt atgtaaggct cacactcggc
    29221 ggcgtttata acaagggtat cgaccttgta tttgttctcg tcaaagttga gcttaatgtg
    29281 cgtcgggaag cctgcaccgc ccattccgca gcagcctgat tgccttacag cttcgcaaag
    29341 actcgccttg tctgttataa caggcggctt tacgtcaggt gatacctcct gctctccgtc
    29401 ggtgtcgatc tccaccattt tgcatacctt tccgcctgct gtgagcagtt cggatatcgc
    29461 cgccactgtg cctgacacag acgagtgaac agggcatgac ataaaagcgt tgctttcgcc
    29521 tatgacctgt cccacaagca ctctgtcacc cttggcaact gtcggctcgc aaggtgctcc
    29581 catgtgctgt gacatggata tcttcactct tgcaggcagc ggcatggcga tagtttcgct
    29641 gttttccgtc ccctttttgt gggaaagaaa aatgctgttg agtttcataa ttttccccct
    29701 tatcatactt tatgactatt caaacctgaa tttatcatca cttatactta tcctgctctt
    29761 tatactgtca gagcaataaa ctacgccatt ttcatcaatg gcaataactt ctatatcttc
    29821 atcactgagc cacctgtcca gctcaccgct tccgccaata aaaatgcagg tagacagaaa
    29881 atcggtttca atgccgctct gccctatcac agtgactgaa acaaggtctg tttcgcatgg
    29941 ataacctgtt gtcaggtcaa aaatatggct ccactttttg ccctgtgctt caaaataacg
    30001 ctcatatccc ccggaagtgg aaacaaatcc gccgccgctt gtgaagctaa ggcagatgtt
    30061 atcgtcatca aaagggtctg tgaccgnnnn nnnnnnaatc tgttttatcc ggcttctgcc
    30121 cgtaaagaag cgttgacgag ccaaagctca ccaccccgca atcggctttt tcactatcca
    30181 aaagcttttt cacttcgtca agggcaaagc cctttccgtc acagcccacg tctatctgtg
    30241 tgcctttttc aatgacacat tcgctccccg aaagctttat attctcatat cccacagcgg
    30301 aaagagcagt ctgtatctcc ctttgctcag gcactctcgg ctcatcgcca gtaacgttcc
    30361 aaagttccgt cactgcccct gcggaaatat ccgctccgcc gtattttttt gtaagctcta
    30421 tgcttttttc tataagctgt gcagtttcct cagaaagctc aagcctgccg ctctcattga
    30481 gccgtgatat ctccgagcct tcctcataag gcgaataaag cttttccaaa gcctttatcc
    30541 tgtccttcac cgcctcagcg tcgccgccgt aaacagcggc tgtgcatggg caatccatgg
    30601 cgaaaaatgt cgcctcgtca ggctttttat ctaaatgcat catcaccgct ccgcacacca
    30661 taaaagccgc cgcacacgct accgatacaa acaaccgcct ttcaggcttt ttcattttta
    30721 tacctcactt gaaattagtt gaaaagtgtg ttataatatc cgaagtatga taacatcggt
    30781 atgaaaattc ttatgtgcaa gcgagcaaaa tgtgttcgca ctctttgata aacgccctca
    30841 gttcaggatt tttccccact gaaacctcga gcctgttcac cttttttcct gccataccaa
    30901 ggatcctgtc aaggtcttca ggctgagcgg aaagatattt tacacagcgt tccatagttt
    30961 cctcagtgtc aagctcaaaa agcttgtggg agttgtgagg attttcagag taaagctttc
    31021 tcaaaagcat ataggtgcat attttcaccg ccatgtccgt ctgagtttca agacccttta
    31081 tgccgctgtc ttttataagc tccctcagca gctcgcttcc ctcgtccgga cagcctaacg
    31141 cagagttttt ttctctgacc ttgactaaac cgaacacatc ttcataggaa gcaccgtaaa
    31201 gctttataag agcaatgatg atattgacgt tctgcttttt gccgtgttca acggctgata
    31261 tgcaagactg gctggtgccg actgctctag ccgcacggct ttgtgtatag ccgctttcta
    31321 tccttgcggc ggtaagcttc tcacctatcc tgagagtcag ctcatctttg ctgtcattgc
    31381 tcatgtaata tctcccatat ataaaatcat attggctttt caaccattat tctattatac
    31441 tataaattta tgtatttgta aatataaaat ctataaaatg acgtatttaa gtgaaaaaaa
    31501 tttgtgcagg ttttaaagca cttatcataa aagaaaaaga aagcaggtat tttaaatatg
    31561 aaaggctaca gaatagcatt tatccgtcac ggaatgacgc aggcaaatct tgacggacgt
    31621 tatatcggta ctaccgactt gccgctgtgc agagagggtg ctgaccagct ttatgaaaag
    31681 atagaaaagt atgattaccc ttcggtgcag aaggtctaca cctcgccttt aaaaaggtgc
    31741 aaacagacta tatctatact ccagcctaac aggcttgtgg ctgagatgcc cgagcttcgt
    31801 gaaatggact tcggcaagtt tgagaacaag accgctgatg agcttatgaa tgaccctgat
    31861 tatgagcagt tcataaaagg aggacttgac aatcctccgc cgaacggcga atcaacaaga
    31921 gaggtcataa accgctgtta tgaagctctg aacatcatca tttccgatat gatgtacgag
    31981 ggtcttacaa atgttgcggt atgcacccac ggaggactta taatgaatat gctggcaggc
    32041 tttggcgtgc caaaaagaaa acctatggac tatgcctgcg atttcggcga gggctttgaa
    32101 gtcatggtga cagcttccat gtggcagcgt tcaaacgctt tcgaggtaat aggaacatat
    32161 ccgccaaaat atgaggaaat gcctgattac actgttgagg attattatac tgacgaagag
    32221 gataagtaag cagtaaaaaa cagaaacgag gggggaaaag gcttgacaaa caaagagata
    32281 aaactgtcgg caagaaaaaa tataagccag aacatgggaa actgcgtatc gctcacggca
    32341 tttttgtttg cggtgtttgt atttcttggt ctgtgcgagc ttgccctttt tatgcttttc
    32401 aaacgcatcg gctgggagta ctttttctcg tggcagggaa taaaagatca tgtttccgta
    32461 aggctattct ggtttcttga cctcattgtc attatacctt ttgcagcttt tgaaatgtgg
    32521 actgtccgca gattttttgt tgatattgca agagcagaca gctatgtggg atcgaggcag
    32581 tatataaact cacattctta tgtatattat tccc
//
LOCUS       DAWEMR010000007        31875 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_579_length_31875_cov_13.533595, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000007 DAWEMR010000000
VERSION     DAWEMR010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 31875)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 31875)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..31875
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_579_length_31875_cov_13.533595"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         9470 a         6706 c         7463 g         8236 t
ORIGIN      
        1 ttttatagtt tgtttaaggt gactaaatcc tccgcaagcc tcgtttttat gttatcttaa
       61 acgtttaaca tccttgacaa aatacagaga aattgatata attaagatta gctgaatgaa
      121 cccattttgg cattttacat aaaattgtgg gctgatttgc ataatttatt gcccgaaatt
      181 tgaacatatt gtacaagaaa tacaatatgt tgtggtcgtt gtgtgcttct gacacgatat
      241 gtgaataatg cacaaatatt ttaacctttg tttgagtata ttacataggt caaaatgacc
      301 aatgcagtgg atgagattag ggcgaagatt ctgtttgatg tgataaagga gccatgcggc
      361 atggacacta tactttcaac tgaagatctg tgccgtgact ttaagatcgg cgacggaagc
      421 gttgtgaatg cgctgaaaaa tatcaacatt gaaatagaaa agggcaagct gacgatcctt
      481 agaggacgtt caggaagcgg taagactact cttatcaata ttcttggagc gcttgacaag
      541 ccaacaaagg gcaaggtcat gtttggcgga cgggatatta caaggctttc tgaaagagcc
      601 agagatgata tacgacggta taaaatggcg ttcgtgttcc aatctgtggc actcatgtcg
      661 ggaatgaccg cttatgaaaa cgttgacttt ggtctaagac ttgcgaaata tccatatgaa
      721 gaaagggata agcgcacaag ggaatgtctg cgaagcgtgg gacttgaaaa gcgaatgttc
      781 caccgaccgg gggaaatgtc cggtggtgaa cagcagcgag ttgctatagc aagagctatc
      841 gcacataagc ctgacataat ttttgcggac gagcctactg ccgagcttga tacggcaact
      901 gcgctccacg ttgtaaagct tttcaaagag ctggtggcga gagatgagat gactatcata
      961 atgaccactc acgaccccag catgatagat atagctgata aggtttatac attagaggac
     1021 ggtgagatag ttgagtgatg aacttgatac tattgtagct gccgacaatg ataaatatat
     1081 gatacggtgt gaaaatctgg tgaagatcta caaaacttct gatgttgagg tggtggcgct
     1141 ccaaggactt gacctggatg tcgagcgtgg agaacttatg gcgatcgtcg gaaactcagg
     1201 aagtggtaaa tccacactgc tcaatatgct tggaggactt gataagcctt cggcgggaaa
     1261 cctttatgtg gacggtaagg atcttctgaa gtttaccgat aaggattata tggagtataa
     1321 aaggaacacc gtaggctttg tgtggcagaa taatgcaaga aatcttgtgc cgtatcttac
     1381 ggctgtacag aatgttgagc ttcctatgct gctcaacggc gagcataaaa gacggcagaa
     1441 agcactggag ctgcttgaca gagtgggact gctgaaaaga aaaaacagca ggctcgacca
     1501 gatgtcggga ggcgaacagc aaagagttgc aattgctatt gcattggcaa acgatccgag
     1561 attactgttg gctgatgaac ctacaggctc ggtggataca aagacttcca atatgatact
     1621 tgatatattc aaggagctta ataaagatga gaagatcact atagtgatcg ttacccatga
     1681 ccttaagctt gccgatcata tcgacagagt ggtggctatc agagatggaa gaacaagctc
     1741 tgagatcgta agaaagcgtt cttatgtgga ggagcttaat gaaatgggag atatcgtgct
     1801 ggttgagaac agcgaggaaa cggctcatga agagcttatc gtcctcgaca gatctggacg
     1861 gctgcaactc ccgaaggact ttatggacgc tcttgagata aaaggcggcg ataaggtcaa
     1921 ggtcgaactt gatgagaacg gcgaaaagct tgtgctgttc agatcggggg ggacgtaagc
     1981 tttattttct ttatttcccg tgggggatca cataatgaag aggttctata ctatttatat
     2041 tagaatgtaa ttaagtaggc ttgattctcg caaaaccaaa agcaatttat ttagaaaggt
     2101 ggattcatag tgaaagacac aatgtttagg cgtgttattg cttccgcatt ggcacttgct
     2161 ctgtgtgcaa gcgcatcagt tccagcattt gcagacagtg aagcggcgga cgacagcact
     2221 ctaggcacgc aggcaactgc tgatgattct gcaacaggcg acggaagctc cgctgccacg
     2281 acggataccg attcgatccg tcagacttct tatactaact atgtaaagaa gtatgccgac
     2341 gctgcaagac ctgaccagac agtcgaggtc ttgggaaagg attacgatcc tgcttcagta
     2401 acagacgctc agatcactgt aactacagta gatggagaga atgatgttat gcagtgggca
     2461 aatcaggagg gttcagtttc ttggacggtg aacattcctg aaacaggtgt ctacaatatc
     2521 aagatgatat atgaggcact tgaaagcaac acaaacgacg ttgagttttc gttgcttatc
     2581 gacggcgaaa gcccatatgc aacagcgtca agaatcacgc tgagcaagag atggatcaac
     2641 gaatctgaga tcaaacagga ttcaagacag aacgatatcc gtcctggaca gatatcgact
     2701 ccatgctggc aggaaactcc tctggaggac atcgacggac tgtttaacga gccgttggag
     2761 ttctatatgg aggcaggcga acatacgata acatttgaat ctgaaaaggc tgagtttgct
     2821 gtcaagagct ttactttcta tcagtatgag gcacctgctg cttacacagc accatcagac
     2881 agcgatctga cacaggctca gggacagaag gtcactcttg agggtgaaac tgctgcctac
     2941 aagtcatcaa gaactcttta tcctacatca gataagagct cttatctcac atcgagcgca
     3001 aacggctcaa gccctactaa aacaagatat aatactatcg gttcaggaag ctggacgcag
     3061 tcaactcaga cagtaacatg ggagttcaat gttgacaagg ctggctacta taagataggt
     3121 ataagaggca gacaggatca gatgagaggt atgtactcca acagacgtct ttatgtcaat
     3181 ggtgaagtac cttgccttga agctaatcag ataaagttct actacgatac agactggagc
     3241 atcacaacgc caaagtctga aaatggcgac gatctttatt tctacctcca ggcaggcaca
     3301 aacactatct ctctggaagc agttcctggt gagatcggtg agatcatggg cgatctggac
     3361 gagcttgttt ataacataaa cagctactat agacagatca gacagatcac aggtcctgat
     3421 cctgatgaat acaacaacta catgatcgac acagctatcc cttctatcgt tcctgatttc
     3481 aaggaatatg ctaagacatt gagagataag aaggctgaga tcgagaagct ttcaggttca
     3541 ggcggaactg aggctgaaac tcttgagaaa atggctatcg ttcttgataa gtgtataaag
     3601 aagcctgacc ttatccctga gatgatgtca cagatcaagg ataacatcac atcggtttct
     3661 tctttcgtaa accagtatcg tgagcagcct cttgaagtcg atatgatcga ggtagctact
     3721 tccgatcagg atttcacttc atgtgacaag tcattctttg gctcacttgg cttcggcttc
     3781 aagggcttta tcggttcatt ctttgaggac tacaacgcac tgtccgatga ggacgaaagt
     3841 gctatggagt gctgggtaat gctcggtcgt gataacgctg aggctctcca gcagctcatt
     3901 tcaagtgaat acaatccaac agctaaaaca aagatcaatc ttaagctcgt tcagggcggt
     3961 atcgtcgagg ctacatttgc aggcaagggt cctgacctcg ctctcttcat gggcggcgac
     4021 ttccctatcc agcttgcagc ccgtggcgtt cttacagatc tcaccacatt cagcgacttt
     4081 gacgaggtaa agtcacgttt tgcagacgac gctactgttc tttatcagta caacggcggt
     4141 acatatggtc ttccatgtga ccagaccttc ccaatgctgt tctatcgttc tgatatcctc
     4201 agcgagtacg atatcgaccc ggcaacagat cttaacactt gggacggact tcttaactgt
     4261 ctgccaacac ttcagagaaa ctaccttgaa gttggtctta tcctccctgt aatgacaagt
     4321 acaggcggaa caactcaggt atctgctatc acagagcctg gtaacacatt cgctatgctc
     4381 cttctccagc agggtcttaa ctactacaac gaagagcaga caaagacaac attcgatact
     4441 caggaagccg taaacgcttt cgatacttgg acaaagttct atacaacata cagcttccag
     4501 cagacatatg acgcattcac acgtttcaga acaggtgata tgccagtagt tatccagaac
     4561 tacacattct ataaccagct ttctgtagcc gctcctgaga tcaagggctg ctggggattc
     4621 cagccagttc ctggtactgt tcaggaagac ggaactatca accacgcagc aaactccaat
     4681 ggttcaggtg ctatcatctt cacaaaggca gcagatcagg agggcgcttg ggacttcatc
     4741 aagtggttca cctcaactga cgctcaggtc aagtacggta acaacatcga gtctatcctt
     4801 ggtacaatgg gtagatatgc aacagctaac gaagaagctc ttcagcagtt gtcttggaca
     4861 acttcagaag taaatcttct tcttgaccag cttaattcac aggtagagat cccgatcatt
     4921 cctgcatctt acggtgtaac aagaaacgtt atgaacgcat tcagagctgt agttaatgac
     4981 tacgacaacg ctcgtgatac actgttctgg tacaataagg atatcaatga cgagatcact
     5041 cgtaagcttg aagatcttgg tctgtacgac aattaatgaa aggggagtaa ctaatggcca
     5101 aaaccaatcc tgaagcgcct atgataaaac agggcaagtg gaagtacaca tggcacatga
     5161 tgaaaaccaa caaggcttgc tatgcaatgc tcctgccttt catgtctttg ttcattatct
     5221 tcacggtagt gcctgtcgta atgtcgctgc caatgggctt tacaaacttt aacatgatcg
     5281 aaactcctaa gttcgtagga ctttcaaact tctatacgct gttccttaac gacgacgtat
     5341 tccttatcgc agtaagaaac actcttatct ttgcgatatt cacaggaccg ttctcttata
     5401 tcctcagctt tatcattgct tggctgataa acgagatgaa cgcattcctc aaaaccttct
     5461 tcacattcgt attctatgca ccttctatga caacttccgt atatgttacc tggcagctta
     5521 tcctgtcagg tgactcctac ggttacctga acgctgtgct tatcgatctg ggtatcctga
     5581 acgaacctgc acagtggctc acagatacaa actatatcct tacagttgtt atcatcgttc
     5641 agctttggat gtcaatgggt gcaggcttcc ttgctatccg tgcaggcttc cagaacattg
     5701 ataagtcaat gtacgaagca ggtgctatcg agggtatcaa gaacagatgg caggagcttt
     5761 tctacatcac tatcccttca atgggaccac agcttctgtt cgctgccgtt atccagatct
     5821 cagcatcatt cacagtcggt gttgttggtc agaacctcgt aggtctgcct tcaacagact
     5881 atgcagctca cacgatcatg aaccacgcaa cagactatgg taacattcgt tacgaaatgg
     5941 gttatgcgtc agcaatatgt ttcgtattgt tcgctgcaat gctccttgcc aataagggta
     6001 tcaactggct cttaggcaag tatcttgact aaaggagggg agtatataaa atggctagaa
     6061 aaagaaaaaa gcaacagtct attgaaaagc tgaaggtcaa agagaaaaag gcgagagtca
     6121 atggttctct tggcggagat atagttattt tcgtattcct ctgtctcctt ggtgcattca
     6181 tggtgttccc gctgtattac tcagttatcc agtctatgaa gcctgttgag gagctgttcg
     6241 tgttccctcc aaagctttat gtactgaatc cgactaccaa caacttctca gatatgttca
     6301 aggtagccgc aagctcatgg caggtaccgc tgtcaagata tatcttcaac agcttcttta
     6361 ttacaatagt aatttgtatc tgcaaccttt tcgtttgctg ctgcgcagca ttcgttcttg
     6421 caaagtgtaa attcccagga aacaaggtca ttaaccagat catcgttatt gccctcctgt
     6481 tctcctcaaa tgcaacatgg atcatgcagt tcctcgtaat ggctaagctc catatgatcg
     6541 atacatactg ggcattgatc ctcccaagca tttcaacatc aatgggtctt tacctcatgc
     6601 gtcagagcat gacaaccatc cacgactcca tggtggaagc ggctaaggtg gacggcgcag
     6661 gtctgttcag gatctgctgg cagatcgtcg ttccaaactc aaagccagct ttgatgacac
     6721 ttatcatctt cgctttccag ggagcatgga acattcaggg cggagcactt atctactccg
     6781 agcagctcaa aacactacct acagttgtac agcagatcac agcagcaggt cttgctcgtc
     6841 agggcgttac atttgccgca gcagttgtac ttctgatacc accactgatc gtattccttg
     6901 tagctcaggg caacgtcatg gaaacaatgg ctaactcagg tatgaaggat taatttgaac
     6961 ttagattata gaaaaggaaa aaaggtgata agtagtgtcg agaaataaaa tctctctaaa
     7021 acgaatgatc tctgcggcac ttgcagcagg tctggcgctg accatgaccg tgactgcttt
     7081 cgcagatgag ccttatacag cctataacta cgactattgg gacgatgcga tcccatcgca
     7141 gagtgcctac cgtgtagaaa agactgtaac aggtgcagat atgggtcttg acaggctttc
     7201 agatccgagt gacccgcttt atataagcga tgacgctcct gcaaagctct cagatgcaaa
     7261 agacctcttt tacgatcagg acaacgatac tttctgggtg tgcgattcag gcaacaacag
     7321 aatactccgt cttgatactg atcttaaggt aacaggctgt tataccggat tcacaggctc
     7381 tacagaaata tctgtaggcg aggacggaag atcgaccttc cttaatccta acggcatata
     7441 tgtcaaaaag agcatctttg atgataagct ttatgtttac atcgcagata cagacaattc
     7501 aagggctgtt aagtgtactg ttgagtcagg cacagaaatg actctggtac aggaatacac
     7561 aaagcctgac acaaagcttt atgattccga aacattcaac ccttcaaaga tacttgcaga
     7621 taaggcagag aatatctacg ttgtttgtaa gtcagtaaac acaggttctg ttcagttcaa
     7681 taaggacggt gagttccagg gcttctacgg tgctaaccga gttgaggtaa cagccgccgt
     7741 tatcgctcag aagctgtgga gaaagatcgc ttcaaacgag cagatctcag caatgacaag
     7801 aaacgttcct gtcgagtatg caaacttcga tatcgacgca gacggattta tctacactgt
     7861 tactgaggct gcaaatacta cgaccgacgc tgttaagaag cttaacccag caggctataa
     7921 catctgggac aacgcagtcg gcgatgagta ttcatttggt gacgttgctt cagagtatga
     7981 ttcaacaaca aataaaactt ataagtgcag acttacagat atctgcgttt cagacaacgg
     8041 aacaataaac gttcttgact atgaaaacgg cagagtgttt cagtatgata agctctgtaa
     8101 tcttctttgc atcttcggta caaagaactc cacttccgat caggcaggaa gcttccttgg
     8161 accaaacgct atcgaggcaa gaggcaacga ggtctatatc cttgacggaa caaagaacga
     8221 tatcacagtt tatactgaaa caactttcgg taatgtcgtt cacagagcag ttctcctcta
     8281 tgacgagggt agatactcag acgcaagagc tgactgggaa gaagttataa agagagacgg
     8341 cggctatgca atggcttatg tcggacttgg taaggcttgc cttaacgacg gcgagtatat
     8401 gaaggctctt gattacttca aaacagctta cgatcaggac gattacgata aggcattcaa
     8461 atatgcaaga gaagaatggc tcagagataa cttcacagtg ataatggtag ttattatcgt
     8521 tttgatcgcc ctgctcatcg taaaggcagt tctcaagaaa aagggcataa agctcatcaa
     8581 gcgcaaaaag aaggagggaa actgatatgt atgaatttac tccggtaggt tggctcaagc
     8641 attgtgtttt ccaccctgtt gaaggttttg aggacctgag atggaagaaa caaggctcaa
     8701 tgaaaatatc gatgctcata gttctcttcc tctttattgc aatggtcgtt gacagacagc
     8761 tcacaggctt ccagttcaat gacagctacg ttaaagtatt caacgttgtt ccgcttatcg
     8821 ttcagtctgt agtttacttc ttcacttggg tactcggcaa ctgggcaata tgcacacttc
     8881 tcgacggtga aggtacactt aaaaagatct gcatctattc tgcatattct cttgttcctt
     8941 acatcgtttg cacatttatc gcagtgatct tctcaaacgt tctggtacag gacgagtcga
     9001 tctggatcac agctatccag tatcttggca tcggctggtc tgttatcctt atggtgcagg
     9061 caatgagagc cgttcaccag tattcattcg gaaaaacact tgcttcaatt attggtacga
     9121 tcatcgcaat gcttcttata ttgttccttg cgatacttct cctttcattg ttccagcagg
     9181 tatatgtatt cggttactca gtttatactg agatcatgta caggatcaga ggctaaaaca
     9241 gaaacggtgg tgtaaataat aaatgcataa caaaagttac aagaaagcag ttgtctttgc
     9301 tatctgcctt acaatgctga tgcctctggg gtcaatggtg gtttcttcca ctgacgagag
     9361 ttcaagccag gctgattcaa gcgccgcagt aaaggatact gacagcagtg tcgattcagc
     9421 ggctgatgat acaagctcag ctgacgcttc cgacgcagca gctactgccg atgaaagcag
     9481 cgataaagct actgaagact cagcagccga tgagaaaaca actactacag caagcaagga
     9541 cgacgaggtt cagcctataa ctgacgaaga agctcttgca atgtgcgaaa agataacaga
     9601 gaacgacaag atagctcttt atcttgacga agaaaacgaa agactttgtc tttatgtaaa
     9661 ggcttcaggt aagtattggt ggacttcgcc tataaatgtt caggctgacc agacaattat
     9721 cgacacagtt aagggcacag caatgaaaaa tgcccagaga aagcagatcg ctgcaagtgc
     9781 cgctatcaga gtcggtgacc ttagacagga aaagagaaca gagtcccctg ctccggttta
     9841 ctccaacaag gcaaaggtaa aatggcagaa aaattcagac ggcgttgttg ctacatataa
     9901 ctacgtttct gatggtgtga agctcaagat acattacgtt cttgaggacg ataaccttta
     9961 tgtttactgc gattcagatg agatcgagga gaaaaacact tctcaggtag acggaaaggt
    10021 gcttacaaag atcgagttct gccctaactt cggcgctgct gattcaactg cgacaggcta
    10081 tatgatagtt cctgacggaa gcggtgctgt aataaactac aacaacggca agaccgagta
    10141 cgctgactat aatcagcagg tattcggcag agattataca gccgttccaa taacagctcc
    10201 aagaaccact cagcaggctt atatgccggt ccttgcaaca gtaagcggtt catccggact
    10261 tgtttgcgtt gcttctgacg gtgagtcaaa cgtttatgct cacgctcagg tatgcggaca
    10321 ggaaaagcag gcttacaaca cttgttactt cgagtttgaa acaagaagct cagactcatt
    10381 cttcatgagc ggtgataact caaacaagat cacagtgttc gagaagaacg gaataaagac
    10441 cgagagattt ggagttcgtt attatcctgt tgattcagac aacggcgagg atcttaacta
    10501 cgctgactgc gctgaggtct acagaaacta ccttatcaat aataggggtc ttacagctaa
    10561 ggctcaggca aataagagcg atctttacgt tgacctttat ggcggcgtta tgaaggatac
    10621 ttctattctc ggtatcccat tcaatcttaa aacagagatc actggcttcg atcaggcatc
    10681 cgacatactt gatatcctca aggacggcgg agttgacaac ataactgtca actataacga
    10741 ctggacaaat gactctatca agaacaagat atccacagag gtatctccgt ccggcaagct
    10801 tggtggttca agcgcatttg ataagcttat ctcaaaggac gataatatga agatcgttcc
    10861 ttcaatgaac aacttcaaga tggacagtgg ttcatgggga tatatgactc ttacaagcac
    10921 agctatcaga gtttcaaatg cttattcaag acagtcatca tacagcccgg ccttcggtgt
    10981 tgcagaaaag ggagtttcac ctgctctgct cacaccaaac aagtactcga atgtgttcac
    11041 agaaatgctc gagagctata ctgatgaaaa gctcacttct atcggcttcg gtgattattc
    11101 aacaaagctt gtttctgact attcaaagaa ggatccttca agcagaagca agaccatgaa
    11161 tactgtcgtt gacggctata agagtgcgag cgagaagata agtacagtat tcgcagacgg
    11221 tgcgaactct tacgttctcc catatgtaac aaacgtttca aacgttcctg tttactccag
    11281 tggattcaac gtaacagatt tcgatatccc gttctatcag atggtcattc acggatatgt
    11341 tgactacgct tcaacaccta tcaataagag ctcaaattca gatgaaacat tcctgctttc
    11401 actggcttca ggctcacaga tacactatga tatgacatac gctgacgctg atacgcttca
    11461 ggatacagaa tatgacgacc tgtattattc aaactatgca ggctggacag atcttgcggc
    11521 taatcagtac aaaaaggtaa gcagcatact ttcaggcgtt tcagattata cgatcacaaa
    11581 gtatgagctg agcgatgaca agaacgttct taccacaact tattcaaagg acggagcttc
    11641 agacgttgtt atcagtgttg ataagaagaa cgcaacagct accgtaggca cagaggtcta
    11701 tgaccttgca gattgtatag aaggaggttt gacagaataa tgagcgaaga gatcaaaaac
    11761 actcctgaag agatcgaggc tgttaaggct gaggatcttg aagatacagt cgaaaaggaa
    11821 aatgacgctg aggctgttac agaagaaatt tcaacagagg agataatcgc agaagagcct
    11881 gccgctgagg cagtcgctcc tgcacctgtg atgaaggaaa agaagcacag actttcttat
    11941 gaaaaaagaa aaggacttta cggctacgga tttatagcga tctgggcgct gggtactatc
    12001 tacttcttta tcctgcctct tatagaatct ctcatttact cattcaacaa aactgaggta
    12061 actcagggcg gaatgaaaat gtcctttatc ggttggaaaa actatgtaaa cgctttccgt
    12121 aaggatcagg attattccaa ggctctggta gcaatgatcg agaatacggt tttgagaact
    12181 cctcttatcc tcatattctc tatctttatc gcagttatcc ttaatcagaa attcaaggga
    12241 agaacattcg caagatcagt attcttcctc cctgttatca tcgctacagg tcctgttatc
    12301 gacattatca acggtaacat gagcacaggc ggatacgcag gcggatctga gcagttcagc
    12361 tcaatgttcg aggctgacct cgttgaccag ttgctgaact tcctcggtat ctacaacatc
    12421 agtgataagc tcactatgat aataaactcg ctgaccaccg acatcctgaa ccttgtatgg
    12481 aactcaggta tccagatact tctggtgctt tcagcacttc agggtatccc gctctcagct
    12541 aaggaagcag cccagatcga gggtgcaact tcatgggagt tcttctggaa agtcacaatt
    12601 ccttatatca gcccaatgat actttcaagc gtggtttata caatcgttga ctcattcgtc
    12661 gatccacaga acgaagttat gacgatcgtt cttaacaagg caagcaactg ggagcatggc
    12721 tactcagcgg caatggcttg ggcatacttc cttatcgtag cagtattcct tgcacttgtc
    12781 gtagcacttg ttaataagtt cgtttactat gaagtagaat aaggggggat ttaagtggca
    12841 actaaaaaag aagaaaaaga gttcgaaaaa gaacgaaagg ctgctattaa agcccgttat
    12901 aagaagatga aagaggaggg tcttgagaaa tatctcaccc ctgagcagat cagctataac
    12961 agaagaagaa gagcgatcgg aatggtttgg ccgttcttca ggttccttat cctgtttggt
    13021 ctgtgcttcg taatcctcta tccactgata ttcatgcttt caactgcgtt cagaccaaat
    13081 gagcagatga acgacccgtc agtagtatgg atccctaaga gcttcacact tgataacatc
    13141 aaggacgttt ggggagtaat gaagtttgaa gatactgttg taaacacagt tatccttaac
    13201 ctcgtggcct ctgtccttca ggttatcacc tgctctatca caggctacgg cttcgctaga
    13261 tttaagttca aaggtaaggg aatcctgttc ggtattgttg ttcttatgat catcgtacct
    13321 ccacagatca caactatccc actttacctc cagtatgctt acttcaagcc agcgtggctc
    13381 gctaaggtat tcacacttgg caaatcaaca tcgcttatca acagcggttg gacaatgtat
    13441 cttcctgcat tgtttgcaaa cggactccgt gcaggtctgt ttatcttcat cttccgtcag
    13501 ttcttcagag gacttcctaa ggagcttgaa gatgctgcat acctcgacgg ctgcagccca
    13561 ctgaaaacat tccttgttat catgtgtcct aacgcaaagt cttcgttcct gacagtattc
    13621 ctgttctcta tcgtttggta ttggaacgac tactatgtat catcatcatt cttcacacag
    13681 aacgacactg ttgctcttat gctgaagaac ctgaatacaa acctgactca gcagctcttc
    13741 gacggtcagg cggtatctgt aagacagata atcgtatggc ttgaagcagg ttgtctgctt
    13801 tctatcacac ctatccttgt tatgtatgta tttctccaga gatactttat cgagggtgtt
    13861 gaaagatcag gtcttgcaaa ctaatttgca aataagctta tcaaaagtcc tgcacttcgg
    13921 tgcgggactt ttttttgcgt gaagcccttt ttatgttcat atatttaaga cttattaaat
    13981 ttgtacatat ataaagcaac gcaattgtgc atattgcttt gaaatgtcag gttaatttag
    14041 tacaatcgtg tatagaaaaa acatcttttt tttgatataa ttactttggg tattattgcc
    14101 catggtaagt tgtttataaa aatattcagt taggagtctg aacatgaata taaaagttga
    14161 tttaaaaaag ctttttgctg ccgcagtatc agcgataatg tgcctctccg cagggggttg
    14221 ctatctgctt cctgacgagg aggaagtact cgatgcgccg accgtgaagg caagcgatgt
    14281 tacatattcc acagttacgg caaagaaaaa agaccttgaa aagaagatag tcgcaacggg
    14341 cactgtcaca gcggaaaatc agtatactct ttcttatgaa aagcagagcg gcacagtgtc
    14401 aaaattttat gtaaaagcag gcgataaggt caaaaagggt gatgttatct gtgaccttga
    14461 tacatatgat cttgactatc agatagaaga aaaacagctt tatctcgaaa aagcaaagct
    14521 taatgttgat ataatttatg agggcggcgg ctcccaggca gagatagact ctgcttatgt
    14581 tgacgttcag ctccttgaaa aagagcttga aaaattgcag gctcagaagg aggattcaag
    14641 cctgaaatca cctatagacg gcgttgtttc gtcactctct gacgtaagag caggcgacaa
    14701 cgtaaatccg ggtcagacta tcgcaactgt tatagatact tcggctcttt atatcgcaat
    14761 acagcctgac gacctcaccc agtatgatat cgacacagag gtgcagataa ggataggcga
    14821 ggattattat gacggtgttg tttttatgac tcctaaaaag cttgcggatt atcaggaaga
    14881 gcagaaagag gatcataaca atgttgacga tacaggtata gattatcagg cggatactat
    14941 ctatgttcgt ttcaaggata ccgcccctgc ggaaacagtg ggtcagcttg cagatacgat
    15001 acttgtcctt gacagcgtaa aggacgctat cgttatctca aataatctga taaaaaaggt
    15061 tgacggcaca aaggtagttt atgtgctgaa aaacggtgaa aaaactgccg ttgaagtgga
    15121 aacaggactt tctacaggct ctcagaccga gataaaatcg ggccttaaag agggcgacga
    15181 aattgttatc agataggttt gaaaggataa gcttatgttc accttattgt tccgtaaaat
    15241 gcgaaacacc aaatggatgg tgctttgcct gctgataggc tttgttatgg cgtctgccat
    15301 gatgagcact atccctattt atatgaacgc ttctctgcaa agaatgcttg taaaggatct
    15361 tgagtcgttc cagacagaat atgatatcta ccccggtgcg tataatacta gctatggatt
    15421 gaaaatggat atctcaggca gtgagcagca gaaagctgtt gagaattata ataacaaagt
    15481 ggaggctaag ttcaaggagc ttggtctgcc tgaaaagctt gataagaagt atataagtga
    15541 cgagtatctc tatgtgcgtt cacttgccgt ttcagatggc aattcgcagg caagattcac
    15601 tctcggcggt atgacggata tctccgacca tatcagcata aagcagggca gaatgttcac
    15661 tgcaggcaag cgttcagacg gcgtttacga gtgcgttgcc acagaaaaag ccatgaaaag
    15721 cacagagctt gctataaaca cagtatatga gatagccgac gtgtttgatg ataacaagtc
    15781 aataaaaata gagatcgtgg gcgtttttga cgttaaggac gaaaatgacg catattgggc
    15841 agagggtctt gattctcagt atacagccgt tgtctttaca gattatgaca caatgctcgg
    15901 ggattgcgtt gatactggcg ttgtgaatat cacaaaggct gtccataact atgcggtgga
    15961 ttatgcttcc atgaatatga ctgaccttgc aagcacaaac gaaatgatag caaagcaaaa
    16021 gacctatttt ggtgaaaatt ccataggctt ttcgatgcct gcctcagata tcctcaagga
    16081 ctatgagagc cgttcatcac agctccagct tgttttgtgg cttttgcaga tacctgtcat
    16141 acttatgata atattctatc tctttatggt gtcgcagctc aacgttgagc aggagcgtaa
    16201 tgagatagcg gtgttcaaaa gccgtggagc aagccgcttg cagataatgg gcatttatgc
    16261 tctggaatct ctcgtactgg gcgttctgac cgttataata ggaccattcg caggtcttgg
    16321 actttgcaag gtacttggtg cgtcaaacgg attcttggag tttgtcaaca ggcgttcgct
    16381 ccctgttcat atgacaatag aagctgtcgt ctatgcagca atagccgtag cggtgttctt
    16441 tgtcacaact atgataccga tctttcctgc caccaaaaca actattgttg agcataagca
    16501 gtcaaaagcc aaaaagaaaa agcacgctct gtgggaaaag gtctgccttg attttatcct
    16561 tgtgggcggt tcagttggct ggctttatta ttacaacaaa actcaggaaa agcttgttgc
    16621 agagggtgta acagacacca ccgcaacagt aaaccctata atgttcgtgg cttccactgc
    16681 ctttatactt ggccttggtc tgtttcttat aaggatatat ccttatataa taaggcttct
    16741 cgcaagaata ggcagaagag tgtggtcgcc tgcacagtat gtttcactga ctaatatcgg
    16801 tcgctcctca acaggcaggg aaagattttt gatgctgttc cttgttctta ctgtatcatt
    16861 gggcgtgttc tttgccaata ccgcaagagc cttgaacaga aatgccgagg aaagcgttgc
    16921 ctatggggtt ggctgcgacg ctgttcttac agagcagtgg tatagcagta aaatagaatc
    16981 tgcacagcag accactcctc aaagcgcatc tcaggcagct caatcaggca caaagcagac
    17041 ttccgaagaa agcgatgaag aagatacaga aactaccata cagtatgtag agcctgtttt
    17101 tgagcgattt gaaaagctcg caggagtgga agatgtcaca aaggttttcc gaaaagacgg
    17161 cgtgaccata tcttcaaaca aaatgaacgt tccaagacag cagacaaagt ctgacgatga
    17221 ggaaaagacc cgtgacgatt tccttgacca aagcgttgac acaagctcag gcaaaagctc
    17281 aacaagcaat gtccagctta tgaccatagt gccaagcgat ttctcaaagg tctgcaaatt
    17341 tgaggacaga cttctgccag ttcagttaaa taactacctg aacgccctcg cagagtatac
    17401 cccgggagtt atcctctctt caagcttcaa gagctacggt ctggagcttg gtgatactgt
    17461 agagtgtaag tggggcggaa acgagccctt tgaagtgacc gtccttgcct ttgttgacta
    17521 ctggccggga ctaagccctt ataaagaggc gaaaaatggc ggttatatgg actttgccgt
    17581 tatgaactac gattatgtga acgttcagac aagcatggag ccttatgagg tatggttcaa
    17641 gctcaaagac ggagcttccg ttcaggagtt ttatgactca atagacaaag caggcattaa
    17701 acccctcacc cttgaatctg caagccagaa gaccatagaa aagaaaaacg accctatgct
    17761 ccagggcatg aacggcgctc tcactctcgg ctttatcatc atcatggtga tgtgcataat
    17821 aggtttcctt atctattgga tactctccat aaagagcagg actttgcagt ttggtatcct
    17881 aagagcaatg ggaatgaagt tcagagagat aataatgatg atagtctatg aacagctttt
    17941 ggtgtcaggc gttgcaatat tcgccgctat ctttatcggc ggcgtaacaa gcgacctttt
    18001 cgtaccgctg ttccagagca tcttcgacgc aagccagtca gtgccgccgt ttgcagtgat
    18061 acctgaacgc agcgactatc tgaagcttta tgcagttata ggagtaatgc tgctcacagc
    18121 ctttgtggtg cttggcagac ttataaagaa gataaagata agtcaggcgt tgaagcttgg
    18181 tgaggattga taggaggaaa ttttatgaca gcttatgaaa agcttacaaa atgttatgag
    18241 tgcgtaaaaa gcaaaataga ctttgagcca aaggtagccc ttgtgcttgg ctcaggtctt
    18301 ggcgattatg caaattccat agacgtgaaa tacaccctca gctataagga tatagagggc
    18361 ttccctacat caacggtaga gggtcataag ggtcagttcg ttttcggata tgttggcaaa
    18421 gtgcctgttg ttataatgca gggcagagtg cattattatg agggctatgc aatggaggac
    18481 gttgtgcttc ctgcaagact tatgagaatg ttgggcgcaa aggtgctttt ccttacaaac
    18541 gcatcgggcg gagtgaacgc aggcttcaag gcaggcgact tcatgcttat aacagaccat
    18601 atctcaagct ttgttcctaa tcctcttatc ggcaagaata tcgacgagct tggaacaaga
    18661 tttcctgata tgagcgagat atataacaaa aagctcaggg atatcatctc aggtgcggca
    18721 gaagatcttg acataaccct ccgccatggc gtttatctcc agctcaccgg accgtctttc
    18781 gagtcccctg cggaaataag aatggcaaga actatcggtg ctgacgcagt gggaatgtca
    18841 acagcctgcg aggctatcgc cgcaaatcat gcaggcatga aagtctgcgg aatatcctgc
    18901 gttgcaaacc ttgcagcagg catgacggat aatcctctca ctcatgctga agttcaggaa
    18961 tgtgcagaca aggctgctcc ccttttcaaa aagcttatca cagaaagcat cataagaata
    19021 ggtaaacagc tttaaggttt tgtaacaact ttttcaaaat aatatgtaac aaatgtgcga
    19081 aaagggctga tgatgtgtac ttttttgcta aactttgtga aaaactattg acaaatcaaa
    19141 cgctttagtt taatatatta ttatgaagta agtatcttca aaaaaatatc aagaatatgg
    19201 agagataatc atatggcaaa attttgtgga cagtgcggag ctcagcttaa tgacaatgcg
    19261 actttctgca ctcagtgcgg tgcaacaata gcacctcagc agcctgcaca gcagaccgct
    19321 tcaccaatgg caaatcttca gcaggcagcc aagaatatcg acatgaacgc tataaaggac
    19381 gctgtttctg ttgaaaacat caaaaatctg aaaaacaagc ctaataagct cactatcgca
    19441 gttctggcag ttctgctcgt gcttgttatt ctggtagttg ttatcctttg catgatattc
    19501 ggcggcggaa gctggaaaac tcctatcaat aacgttatgg acgttctcaa tgacggcgac
    19561 ggtaaggctt atgaagagct tatgcctcct tattataagg atatggcaga cgcactggac
    19621 ggtatcattg atataagcga cgaggatttt gatgaagagg ctgaaaagca gagagataga
    19681 cttacaaaga aatacggcga tgacgctgag ataagctatg aggtagttaa aaagactaag
    19741 gtagactcag acgatatcga cagctataac gaagtttcag acgttgcgtc aatactcagc
    19801 agcaaaaagg ctaagaagta taagcttaca aaggcttata aggtaaagat caagtatact
    19861 atcgaaggcg aagacgctga taagaccgac aagacaactg ttattgttgg aaagatggac
    19921 ggcacatggg ttcttctgga cgagggcgac cttccaataa gcacaggttt ctaagatata
    19981 taaacgatca gtaacgggcg gagaattttc cgcccgtttt tgaaaatgcg gtgtttgcac
    20041 aatgaaaaaa atatcagtgc tgtgtatgct tttgtgtgtg gcaatgctct tttgctcctg
    20101 cggagaggaa gaaagagcgg tcctgggata tgaacagccc gtggcgacac ttgtcacagc
    20161 ggcgcagtat agcgatacgc agtcatatct ttcctgcttt accccagaag cggaggcgga
    20221 gtataaaaac agcgacagct ataacgaagc ccttgcggaa acgcttctca caaggggcga
    20281 ggaaaaaacg gagctttctt ataagctcag caataaaaaa gagcttgaca gcgacggctt
    20341 gaaagacctt gaaaaaagct ataaggaaag ctatcacaaa aatgtgacta tcaaaaaagc
    20401 tgttacaatg acagcaaagc tcacagagac tactgacagc ggcaagctgt ctgccagccg
    20461 tgagataaca gtggtcaaaa tagaaaacaa ctggtatatt ttcggaaaag ttatcgaaaa
    20521 atttgatctc agccaaaagg gctgagggga tcaataataa tatgaaaaga gggtattaat
    20581 atggaaaatt gtccaaaatg cggtctgcct gttgccgacg gtgcaaagtt ctgcgctgcc
    20641 tgcggaacaa gaatgccaca gaagcttata aagagcaagg tctgctcagt gtgtggaaat
    20701 acacttaatg cgggggctaa gttctgcaac gtctgcggaa cagctgtgcc gcaggacgaa
    20761 acgcctgccg acgagcctgt agtggacgag agaaatcctg aaatggagtc tatcgaagtg
    20821 cctgagctgc attttgacga tactcctgtg caaaatcaaa ctattccgcc aacgactcct
    20881 gatatgccga ctatggacag cgtttatctg ccagggcaag agcctgcaaa gcctgttcag
    20941 cctgcacagc cgacagccga ggaagttgca atggcttcaa gaactgtgga aatacctcag
    21001 acgcctacag ttgctgaaac tccaaacgtt gcaccacagc cacagcctga aagagttctt
    21061 acaccagagg gctatcagca gactgctcag cctgtaaaca atgcaatgcc acagatgaat
    21121 cagggtggac agcctatcca gccaaatcag atgaatcaaa tgaacggcgt tacacctgga
    21181 aagggtgcag gcgcacttgt gccgataata cttatcattc ttatacttgc tgttatctgc
    21241 gttgacgttt tctggctttt cagagatcag atattcggca aaaaggacga aagctcaaat
    21301 ggctgtgcag tggtaactct cacggacgaa actgactatc ctgcaaacga agcatttttc
    21361 tttgaagaat agaatataat gtaaaaaggg acgtttcaaa gcgtcccttt ttttgtgtat
    21421 caacacagtg tttgcgtaaa aaataaaggc gacccaaggc cgcccctaca tttaatcttt
    21481 catgctctcg attatctcat tagctttctt gtaaatgaca gcaggctctt cgcctatcct
    21541 gaactcgccg tcctcgtaaa gcacctttcc tgcacacatc gtgagcttta cgttctgctt
    21601 tgagccgctg taaacaaggt ttttgcctat gttgttaaga ggctgcatat taggctgaaa
    21661 aaggtcgata acgataaggt cagcctgctt gccctctgcg ataatgtcgc agtcagaaag
    21721 ccccattgcc cttgcagaac ctacagttgc cgctttcagc accgagtcag cagggcaggc
    21781 agaagcatca tcgcaagcta tcttctgcaa gcctgtcatg aggaacattt ctctgaacat
    21841 atcaagacag ttgttgcttg cagggccgtc agtacctata gcaatgttta tgcccatatt
    21901 ctgcattttt acgataggcg caatgccgct cgcaagcttt acgttagagc ctgcacagtg
    21961 aacagcccat acgcccttgt ttttgaatat ctcaaggtcc ttgtcgctca tgtgaacgca
    22021 gtggaagcct gcaccgccaa agtcgaaaat tccaaagctg tcaaaaagct cagttggcgt
    22081 cattccgtat ttctctatgc actctctgac ttcttttgca gtttccgagt tgtgcattgc
    22141 aacaggcgct ttgtattttg ccgcaagctt gccaacgccc tccataagct ccttttcgca
    22201 ggtgtattct gcatggaagc caagcataaa gcttataaga gggtctttgc cgttgtattt
    22261 cagatagttg ttttcaagcc tttcaagatt agatttgtca ccgctcaccg caccgcaaag
    22321 cacagagcgg aagccgcttt ctatacaggc ttttacataa tcgtcaagct caaagtacat
    22381 atcaaatgcc gccgtacagc ctgttgtgag atattcaagt atagcaagct tcgtgagcca
    22441 gtaaactttc tctccgtcaa gctttgcttc cattggaaag atctgcttaa aaagccagtc
    22501 ctgcaaaggc atatcgtcag catatgaacg cagaaaagtc attgccgagt gagtgtgcgc
    22561 atttttgaat gacggcataa ggagatttcc cttgaggtca tattcacgtt caaaaacctt
    22621 tccgtcaagc tcctcagcct taggcttgcc aacgaaaact atctttgaac cttccgtcca
    22681 gacctctata tcctgcgttt ctatgccgtt ggcaagggtc attaccctgc cgccgaaaaa
    22741 tcttatcatg gctctttatc ctcccttact ctatttcaag aacagccgtc tgctctccat
    22801 ggcgtgagat aactctcggc actcttgcag atattccgca aactatctcg tagcctatcg
    22861 ttcctgtcat tttgccgata tcgtcggcgg ttatcttttc atttccctgt gcgcctatga
    22921 ggacagcctc gtcccctgcc tttacgtcct ctacctcagt tacatcacac atgaactgat
    22981 ccatacatat cctgcctgtg atagggcatt tctttccatt gataagcaca tatcccttgt
    23041 ttgaaagcgc cctgggatag ccgtctgcat agcctgccgt aacagttgcg agctttactt
    23101 tgccatttgc agtgtaagtc ctgccgtagc ttacgtcagt gccattgtct atgtatttta
    23161 cctgcgcaac agccgcctta aattccatag ctggctttgg ctcaaaaggc agagcctttg
    23221 cagggtcagg ataaagtccg taaagtatta ttccaagcct tgcaagagag cttctctcat
    23281 tgtaatagta tactccgccg gctgagttga gaaaatgcac tttttcaagg tgaagcccct
    23341 ctgcttcgca cttgtcagca acggaaagta tcttctccgt ctgcgccttt gtgtatttct
    23401 cgtcctgctc tctcaggctg tcagccaccg catagtgggt gaaaatgccc tccagcgcaa
    23461 tgttttcaag ctttgatatc tctttaagct caccgtatat cttgtcggtt tccccgtgaa
    23521 gacctatcct cgtcatgccc gtgtcgatag cggcgtgtat gcgtaccttg ccgtcagcgt
    23581 tttctgaaag tgctttagca tatgaaagct ctgtgcaagc ctgaatgatg tcaagtcttg
    23641 tgagccactt cgcattctca ggaggtgtgt agcccaaaat gagtatctcg ccctttatgc
    23701 ccatatccct gagcctgtgg gcttcgtgta tgttcgatac cgcaaaccag cgaatgtcca
    23761 tctcgttttc aagaaacgga actaccccgt tgtcaaaatg tccgtagcag ctcgccttta
    23821 caacgcacat tatctctgtt ttttccccta tgagcctgcg gtattctttc agattgtatt
    23881 caatgctgtc aagatctacc tcagcccatg ctctcttcaa aaaatccatt ttatttttct
    23941 tccctttcgg ttttataatt gtgcctttgc acaaaatatt tgtcattttc tattgaaaaa
    24001 gataaatcaa tgtgttataa tgttcttatc atgggcattg tgtcgccaaa tctttcagga
    24061 ggctttgatg tgagtaatat cgtccccgca gaagaatttt tctgcaatcg agatacctct
    24121 tgctgcttta caggtcatag ggacagggat ctgcctttcg gcggcgatgt ccataagcag
    24181 ggtatgaaaa atctcctgag tatgctcgtc cttatgataa aggaagcata tagcgaaggc
    24241 tgccgtactt ttatatcagg tatgtcaaaa ggtatcgacc tgcgctgtgc tgagatagtc
    24301 cacagccttt cggctcagcc cgaatataag gatttgaagc tcgtgtgcgt tctgccctat
    24361 gcaggtcagc gtacagagat gaaaacaccc ttggacagct atacatattc gcttattctg
    24421 aactcttgca gtgcggtcgt gataacaagc cccaagtatg aaaacggctg ttataaaaag
    24481 cgaaacagct ttatggttga acattcctct cgtgtgatcg gggtcataaa agagaaagcc
    24541 aaaggctcag gcactttgca gaccataaac atggcaaagc gtgcaggcct tacgctgaac
    24601 atcatatgcc ttgacgataa tcctgttttt tatatcgact cagactcaga cagccgtcct
    24661 atttagttta cacctaaatt gcaaattgtt caatagtttt cgagaatttt tccgaaaaag
    24721 tttatatatg aaaatatggc ggacagtatg cctgcctgta tatatttgaa agtaaggaga
    24781 tcaataacat ggctcagacg aaagctcaca agaaaaaaag aagatctcag gctcctatcg
    24841 ctctggtcta cttcataacg atgataatat tcctctttgt tatcggcatg cttgcactgt
    24901 atgccctcag agagtttggt gttatcggca atgatgacag cgacagcgac gcaggcataa
    24961 aaagcccggt ttataatacc ctttttgcaa gagtgaacag caagggcgtt cttgcagacg
    25021 caacagtcat aagagttgca ccggacgcaa agaagataat cataatgcct gtttcagcgt
    25081 tcacagtgag cggaagccag acaatgcgtg agatacacga agagggcggc atggtgaagc
    25141 ttgaagcagc cgttgcagac actttcggta tcactataga caattatgtt tctctcacaa
    25201 atgacgcatt cgagaatgcg gcggatattg tgggcggaat tacatatact cctgatgagg
    25261 aactttacta tctctctcag gataatgacg agaacgatat cgctatccca agcggcgatc
    25321 tgactaacct cagcggtcat cagataagac ttatctgtca gtatcctgtt ttcaaagagg
    25381 gcaggaacgg taatatgaag tttcttggaa ctgcggtgac aatgcttata aacaacgctt
    25441 tccagcagac gaatatcact aaggataatc ttgataactt ctataatata ttcaccgcaa
    25501 acagcgatac cgattggaca agtgcacagt ataaggaaga aaagtcgtat ctcaaggata
    25561 tgctggatca gaaccttact cctgcagaag ctcttgtgcc agagggcgag tggacggacg
    25621 attctcactt taaggtgtct gataagttca aggagagcgt tcagacaatg ctcaaagata
    25681 ctgcaccgtc agcagtggag aagtaaaaac gtaaaggctg aaattttaac agggggcgat
    25741 ataacgtgaa acttctggtc ataatcctta ataaaacaga cgtgcttgac tatttgctgg
    25801 agggactttc agcagcaggc ataaagggtg cgactataat cgagtcgtca ggtatggcaa
    25861 tgaccctttc aagacttgac agcagctttc tcagcgcctc tatccgagga cttttcagcg
    25921 gtgacgagga taacagaact atcctgtcgg taataaaaaa taatcagctc gataccgcac
    25981 gaaagatagt ttataatact gtgggcgatc tatctcagcc caatacggga ataatgttca
    26041 ccgttccgct ggatttcgtc gagggaacag tgaagaaacc cgtggataaa gaaaaagcaa
    26101 aacctaagga tatccctgag gagaatgaaa agaacgtcag ttgacgaaat gtttacagac
    26161 tattaacata atatttaatt cttttcatta aaaacttttg aatgtccttg caaaaaactg
    26221 ttttgtgtgg tataataatc acaataggct tttaatgaaa gactggaact tgacttccgg
    26281 tcttttggtt ttgtatgggg ctttttttca tggcaaagcc tgatgacttt gctttgggaa
    26341 gcattaacgg aggtgctgta tggaagtaaa gaaaactacg gtagaaaaag taagagagct
    26401 tgcacagcct atcgcagacg agcttggctt tttcctttgg gacgtaaggt ttgaaaagga
    26461 gggagcaacg tggtacctca gggtgcttat cgacaaggac ggcggcatag atatggacga
    26521 ttgcgaggct ttcacaagac cgatgaacaa ggtgcttgac gaggctgacc ctatatctca
    26581 gagctatgtg ctggagtgcg gaagtcccgg acttggcaga gagcttagaa gacccgagca
    26641 ttttgaggtc tgcataggcg acgttataag agtaaggctc ataagacctg acgaaaacgg
    26701 tgaaaaagag ttcgttgtgg gtcttgtggg atatgacaag gataagatac tttgtcagaa
    26761 gctggacgag gatatgaacg gcgtggagaa tgtagagttc aagcttgcag attgtgctta
    26821 tgtaaagctc aatgatgacg cagacctgtt ctgaatgaag aaatttaata aatatgggca
    26881 aagcccgaat caagtgtgaa aggattgatc agaaaatgaa taaggaaatg accagcaaat
    26941 tttttgaagc tctgacggat ttggaaaagg aaaataatat tggcagcgat atacttattg
    27001 agaaaataaa atcaggtatc gaaaaggcta tcaagaagga ttatccttac tgtgagaata
    27061 tcagagtgga tatcgaccct gttacaggaa agctcgatat aaggatactc aaggaagtta
    27121 tggacgttat gtatgttgac gaccctgaca acgagatctt ccttgacgaa gcaaaggtct
    27181 atgaccctaa catcaaggtg ggcgatatgg tggagatacc tctcgaccct gcaaagttcg
    27241 gcagagttgc cgctcagaat gcaaagcagt ctatcaagca cgatatcaag gattttgaaa
    27301 gagcaagact tatcgagcag tatcacgata aggagcatga gtgcgtttct gccacagtcc
    27361 agaaggtcga gcctgctact cagaacgcta tacttactat cgacaagaac gaagtttatc
    27421 ttgtaagaaa cgagcagata ccaggcgaaa cactcaaggc aggagatatc atcaaggtat
    27481 atgttgtggg tatcgcaaat cctgacagaa agccttctat caagatatca agaactcaca
    27541 gagagcttgt taaaagactt tttgagcttg aagttcctga gatagcagac ggcactgttg
    27601 aagtaaaggc tatttcaaga gttgcaggcg caagaagcaa gatcgcggtt tattcaaagg
    27661 accctaatgt tgacgccgta ggcgcttgta tcggtcctag aaagtcaaga ataacttctg
    27721 ttgtgaacga gcttaagggc gagaagatag acgttatcaa ttggtgcgaa gaccctgcac
    27781 agtttatcgc aaaggctctt gctccggctg aggtaataaa cgttgttctc agcgaagagg
    27841 agggcgtaag agagtgccgt gttactgttc cagataacca gctctcactt gctatcggta
    27901 ataagggtca gaacgcaaag cttgccgcag gtctgacagg ctataaaata gatatcgtat
    27961 ccgaaagctc tgcaaaggct gaggctgctg atgatgaaaa ctaagaagat accaatgaga
    28021 atgtgtcttg ggtgcggtga aatgaaaccc aagagagagc ttatcagagt ggtgaagtca
    28081 aaagaggggg atatctccct tgacctcaca ggcaaaaaat caggcagagg cgcttatata
    28141 tgcaaaagcg tcgaatgctt tgaaaaagca agaaaagcaa gaaagtttga aagaagcttt
    28201 tcctgcatga taagcgagga tatatataac agcatggagg gcgagcttag ggaaaatgaa
    28261 taataaaata gggcttgtta caatgtgcag aaaagcagga aagcttgtaa tgggtatgga
    28321 tatggtgaag gacgcctgca atacaggcaa agcctgtgca gtgtttgcgg caacggattt
    28381 ttcacctaaa tctcttaaag agataaagtt caactgctac aaaaacggga taaagcttta
    28441 cagccttggc atgacaatgg acgatatcca ttttggtctt ggcaaaagaa caggcgttat
    28501 cgctatttgc gagggaggct tcgcaaaggc ttgcgccaaa gggcttgagg agatccctat
    28561 agacgaaaat gagttttata gcatttagca tttattgagg aggtatttta tagcctatga
    28621 gtatgaaaac aactaaatat aagcttagcg atatggctaa ggatctgaaa gttacaaata
    28681 atgaccttat cgagtgtctt ggcaagctcg gcggagagcc aaagaaaaca cagtcagtgc
    28741 ttacccctga ggagatctct tatgttctgg agtattatac acagaacaat caggtggaca
    28801 gctttgatgc tttctatgca aataacgtaa agcctgccaa ggaagaaaag aaggcagata
    28861 agaagcctgt aaaggctgag aaaaaggaga aaaaggctga aaagaagcct gagcctaagc
    28921 cgactccaaa ggcagaagca aagcctgagc ctaagccggc tccaaaggcg gaagcaaagc
    28981 ctgagcctaa ggcagagcca aaggtggaaa agaagccgga ggtcaaggcc gaaacaaaac
    29041 agcctgcacc tgaacagaag aaaaagcagc cagctccaca gaagcctgca aagaagaaag
    29101 agcatggcgt tcgtcagcag cttggcggtt tttctgataa gaaagaagct tcaggcggat
    29161 atactatctc tgaggataac gattcgttcg gcacacagag aactatcgac acaagaggaa
    29221 gctatataga gcttgataag tataacgaga agtatgataa ccttgcaaac tcaaagcaga
    29281 acaagagcaa ggataacttc acaaagaagc agaagctcac tcagaaatct cagcagagaa
    29341 agaaacagca gttctcacat aagaaggaaa ctgagagcga aaagctccgc agactggagc
    29401 ttgaaagagc aagaaagcag cagctcaagg tcatgatacc tgacgagatc gttgtttcag
    29461 agcttgcaag cagacttaaa gtaacagcta ccgaggtcat caaaaagctt atgggtcttg
    29521 gcgtaatggc ttctatcaat gaggtggtcg actttgatac cgccgcactt gttgcagaag
    29581 agcttggcgc aaaggtcgaa aaggaagtac acgttacaat agaagaaaga cttatcgaaa
    29641 ctgacgaaga tccagaggag agcctgcagg aaagatgccc tgtcgttgtc gttatgggtc
    29701 acgttgacca tggtaagaca tctatcctcg acagaataag aaacgctcac gttactgaca
    29761 ctgaggcagg cggtatcaca cagcatatcg gtgcttatca ggtagagtat cagggcaaga
    29821 agataacgtt cctggatact ccgggtcatg aagctttcac agccatgaga gcaagaggtg
    29881 cgaacgttac agatatcgct atcctcgttg ttgccgctga tgacggtatc atgccgcaga
    29941 ctatcgagtc tatcaaccac gcaaaggcag cgggcgtttc aataatcgtc gctatcaata
    30001 aaatggataa agaaggcgca gaccctgaca gagttaagca gcagctcaca gagcagtccc
    30061 tcgttgtcga ggaatggggc ggcgatgtta tcgcagttcc tgtttctgct aagacaggta
    30121 tgggtatcga cgagcttctt gaaaacattc tcctcgttgc agaggtcaag gagcttaaag
    30181 ctaaccctga cagacttgca agaggtactg ttgtcgaggc cagacttgat aagggtaaag
    30241 gccctgttgc tacactcctc gttcagaatg gtactttgaa gtcaggcgac gttataatcg
    30301 caggcacttc tgtcggacgt atcagaacga tgacaaacga taagggcaga agcattaagg
    30361 aagccggccc gtcaacacct gttgagataa caggtctggg cgaagttcca agtgcaggcg
    30421 acgttttcaa cgcagttgca gacgaaaagc ttgcaagaga gcttgttgag cagagaaagc
    30481 atgaagctaa ggaagaacag ttccagcagc atcagaaggt aactctcgac aacctgttca
    30541 gccagatagc tgagggagaa atgaaagagc ttcctatcat cgtcaaggct gacgttcaag
    30601 gctcagttga agccgttaag cagtcacttg aaaagctttc aaatgacgag gtcagagtta
    30661 aggtcatcca cggcggcgta ggcgctgtat ctgaaagcga cgttatgctc gcaaacgctt
    30721 caaacgctat catcgttgga tttaacgttc gtcctgaccc tgttgcaaag cagaatgcag
    30781 agcagagcgg cgttgatatc agactttaca gaataatcta tgatgcaata gaagaaataa
    30841 cagacgctat gaagggtatg ctcgctccta agtacagaga ggtggaaaca gcccgtatcg
    30901 aggtaagaca ggtctacaag atatccaatg ttggtactgt agcaggttct tacgttcttg
    30961 acggtaaggt gggcagaaat aacgagataa gagtcgtaag agacggtatc gttatcgccg
    31021 aggataagat gtcaagcctt aagagattta aggacgacgc caaggaagtt gccgcaggct
    31081 ttgagtgcgg tatcactctt gaaaagttca ccgatatcaa ggagggcgac attttcgagg
    31141 cgttctatat ggaagaatac agagattgat aatgatgctt acgggacgga cagccgcccc
    31201 gtaagagcat atttaagcag gagataaaga tattatggca ggttacagag caggacgtat
    31261 gtccgaagat ataagaagaa taatatcggg caaaatgaga gaccttaaag acccaaggat
    31321 acacggagga gatatgctca ctatcgtccg ctgcgacgtg gcaggcgacg gctcattctg
    31381 taaagtgtat gtttcaagct ttgagggcta tgaaaaggca aaggaagccg ttaagggctt
    31441 tgagtcggca acaggatttc tcaaaagaga gatatcaaac gtgctgaagc tgaaaaaatg
    31501 ccctgagctt aaatttatcg cagataattc tatcgagcat tcagcaaaga taaacaagct
    31561 tcttaaagaa gttctgcccg aagaagaaaa agaagagaaa gacggcgaag agtaatagag
    31621 gagaaaggat attatgacat ttcaggatat tcttaaaata tttgaagaaa atgacaattt
    31681 tcttatttta actcataaaa gccccgacgg cgatacgctt ggttcaggct ttgccctgtg
    31741 cgcttttctg agagatatgg ggaaaaaggc taacgttgta aattccgaca actttcccga
    31801 aagatatgat cttatctatg agagctatta ccctcaggaa tttgatgtga agtacgttat
    31861 cgcagtggat atcgc
//
LOCUS       DAWEMR010000008        28174 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_696_length_28174_cov_12.383726, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000008 DAWEMR010000000
VERSION     DAWEMR010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 28174)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28174)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28174
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_696_length_28174_cov_12.383726"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10645..10654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14093..14102
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8223 a         6109 c         6122 g         7700 t
ORIGIN      
        1 cattatagag tgctttaaat gttatatatc aaatttcaca gcctttttca cactcacttt
       61 cattattcaa ataattttac aaaattacaa ataccccctt gacacttctc ccgttctgca
      121 ctataatact acacaagaga acttttttag cgttgtgcat acgttaaaat gagcataata
      181 aagctcagaa aataagcttt tttgtgagat gtgccgcaaa agtgtcctgt tatttgttaa
      241 agtgtagaca aacggcgcat aatatgatat aatgtagggt gagaaatgag cggtttaaaa
      301 caggcggtca gaaacactgc caagatcata atgttcaaag gagtatatcc cctttgctat
      361 aaagtgtcaa gccttagacc tttgagaaaa aacaaggtca tctttgccga gataagaagc
      421 ggtacgctga cggatagctt ccgctatatc tatgaggaga taaagaccag aggacttgat
      481 ccgcaggtct attacgtcca taataataag ggcggaatgg gctatctgct gagatatctg
      541 aagcttactt ggctaatggg aaacgtgggt tgcgttttgc ttaacgatac ctgtaatctg
      601 ttcggcgctt ttaagttcag aaaagacaca aaggtcattc aaacatggca ttcctgcgga
      661 gctttcaaaa agtggggaga aagtatcacc gacctctctt tcggcgagtc gctggaagaa
      721 ttgagaaagt tccctgccca tacaagctat acactttgta ccgtcagcag taaagagtgc
      781 atatgggctt tcaaggaagc tttcggcttt gaccttgata ataatagcgt gcaggcaata
      841 ggcgtatctc gtaccgactt tttctttaaa gaggaaaatc gtgtgaaagc ctttgaaaat
      901 ctctataaga attgcccgaa cgcacagtat aaaagagtcc tgctctatgc cccgacctat
      961 cgtggggacg ctgataaggc ttatatcccc gaaaagctgg atataaaagc ccttaaagag
     1021 aatttcggca gcgagtgggt gcttcttgta aaaagacacg gctttgttaa aaaagagtgg
     1081 gatatccccg aggattgtca ggacttcgcc tttgacataa cagaacatat gcctatcgag
     1141 gatcttcttt ttactgccga tatgtgcata accgattatt cttcgcttat ttttgaatat
     1201 tcgctctttg aaagacctat gatattctac gcttttgacc ttgaccagtt ttatgactat
     1261 agaggctttt attatcccta tgactatagc ttcctcccgg gaccaatagt taagaccaat
     1321 gaggagctta taaacgccat aaagcaggga aaagccgacc ctactgtcat ttctaatttc
     1381 agagaaagat ttatgggcgc ttgcgatggc cgttctaccg aaaggatagc cgattatatt
     1441 atgtcacaaa acagcgaata ctaatttatg taggggtgaa cagcgttcgc ccgttgactg
     1501 ccgaaacttt atcggcgtat cacaatgatt tatttgtcag atacattatt ataatataaa
     1561 tatcagtaag gagtttgtct atgatttacg gtgcaatgct cgcaggggga tcaggtacaa
     1621 gagttaaaag ctcgaaaata ccaaagcagt ttatcgagat cggaggtaag ccgatcataa
     1681 tctataccct tgaaaatatg ctcaaggtaa agagatttga ttatatctat atcgccgtcc
     1741 ataaggacta tgaggactat atgaacgagc aggtgaaaaa gaatattcct gaaagcgaaa
     1801 aggtaaggat aattctcggc ggcaaggaaa gaatggatac tataaataat gtcaccaccg
     1861 ctatcacaaa tgataacgga attcatgatg acgatgttat cgttatccac gatgccgtaa
     1921 gaccttttgt tacagagaag atactcaacg attccataga ttgtgccgca gaatacggtg
     1981 cttgcgtctg cggacttcca tgtgcagata ctatcctcca ttcaaaggga ggagagtatg
     2041 ttgaagacat ccctgccaga agcgagcttt acagcggaca agctcccgac agcttccgcc
     2101 ttgcccactt tatccaaatg caggataacc tcactgagga gcagaaaaaa gtcatcacag
     2161 gtacttcgca gatatgcaca atgaataacc agcctatcca ccttatagag ggcgacgcta
     2221 ttaatttcaa gataacaaca gacagcgacc tgcttattgt cagaacgctg cttggcggta
     2281 aatagaaagg acttttttat gaaaagctta ataaacatac ccgaaaagat gaaagcactt
     2341 gtgctttatg gcgtgggcga cctgagagta actgaggtag acgtgccaaa gcttgaaaaa
     2401 ggcaccgtat tgctgaaaat aaaggcgtgc ggaatttgtt caagcgatat gccaagatgc
     2461 tttgtaacag gaacttacca cttcccgacc atacctggtc atgagttttc aggtcagata
     2521 gttgcagttg gcgatgacgt tgacgaaagc cttctcggca aacgttcctg cgtgttccct
     2581 atgctccctt gcaagcattg taaagcctgc aaaatggagg aatatgcaca atgctcaggc
     2641 tatagctatt tcggttcaag acaggacggc gcatgggctg agtacctcgt tgttccagta
     2701 tggaacttag ttccgttcga cgataccctc gattataaaa ctgcagcgct ctgcgagcct
     2761 gccgctgttt caatgcacgc tgtaaatatc ggtgacatca aagagggtca gaacgttgtt
     2821 atcataggca caggtacaat aggctttatg atagccctct ttgcaaaaga gagaacaaag
     2881 accggtaagg tggttatcgc aggtcgttca aagaataagc ttgagtatgc aaagaagctg
     2941 ggctttgacg ttgttgatac aagcgttgac gacctccctg cacaggtgaa gaagataata
     3001 ggtatcgacg gagcagacgt aacatttgaa gctgtcggct caaacgaagc tatcgcttcc
     3061 gctatcgagt caacaggcgc tcttggcagt atcgttctcg ttggcaaccc gtcaacagac
     3121 cttatcctgc ctaagaatat ctattggtca atactgagga agcagataac agtcaaaggc
     3181 tcatggaatt ccagctataa cagcagagtg aacgattgga agaaagccct tgaagttttc
     3241 gagcatagcg acgtaaactt tgccgatctt atcacacata cattcacaat agaggagaac
     3301 gaaaaggcat tctccgctat cagaaacaca ggcgagttca ccttaaaggt aatgttcaca
     3361 tttgacgatt aaacagtctg ctgtttgtaa atttgagtaa aaattctgta gtcgcttttc
     3421 aagacctgaa atgaaagttg agagggatag ataaaagcgg cggaaagtaa ggaatagttt
     3481 taaatggata atatgactcc cgattttgaa gagatatacg ggaacagaag aaagactatt
     3541 gagagattaa aagagagaaa caaaaaattc gcagagctta caagagtaga gttcaagaga
     3601 gttatccttc atatcgaggg cgagcttgtg ggctttgaaa aagacgagct tgaaaacttg
     3661 tcagcaagat ttgtcacaca tgatagaact tgtatgttcc ctgcccagca ttttgaagta
     3721 agcggaactc attttcatct gacagtgaat ataatgtccg ccaataacga agcccctatc
     3781 gccacaggcg actatgccct taaaatattc acaaagtata agggcatgaa taaaccgcag
     3841 atatcaacag atacagtgga ttatgtcctc agtggcagta cgcttctcaa gctcacctat
     3901 gataacgatg gcagatgcct gtcaaaacag gtgcttacga acgagtatcc ttgtatcata
     3961 tccgataaag tggaaactat agaggacgat acaaaggcga accctatgaa cttcaagctc
     4021 agacgaggcg cagaaaacct tttctatgcc gagtctgcaa gcgatatgga tacactcgaa
     4081 tataagcttg ccgtcaccta taaagagcct aagccttctc agaccttgcc caagcgtatt
     4141 gcgaaaaagc ttcaaaaccg taagaagctt atcataagag atgtttacgg cttaaagata
     4201 tggtgcttta aaaaggcgtt ctcgttcttt aagcattttg ctaagaaaaa cggggatatt
     4261 atcctctttg ccagcggttc gagagccgag ataggcggaa acgaaaaggt catctatgac
     4321 cgcatgatag aaagaggact tgacagcaag tataagttca gatttgactt caaagcaagt
     4381 atcactgtca ataggtcgat acctgccatg ataaggttcg tctactatct tgcaacctcc
     4441 gatatcattc ttatagatga ttattaccct gacatctacc ttgtggatta tcctaagaac
     4501 gtcaaggtgc ttcaggtttg gcacgcctgc ggagcgttca agtcattggg ctttgaaaga
     4561 ttgggcaagc cgggcgcacc tccgttcaat acaagggtgc ataagtgcta tacccacgtt
     4621 cctgtaagct cctatcattc tgcgctccac catgcagagg gcttcgctat cgacgaaaag
     4681 aagttctatc ctgtgggtat cccaagaacg gatatcttct ttgacgatga ctataaagcc
     4741 aaaacaagag agcagatgct ggaagttttc cctgagtgca aaactgcaaa aacagtttat
     4801 atgtatgcgc cgaccttcag aggaaataac gcaaataatg ctcatttccc atttgataag
     4861 cttgacctta cagcatgggg taaatttctt gatgaaacgg attccgtcct tatcgtaaag
     4921 ctccacccgt ttgtaaaaag gcgtatcgaa atacctgacg agtatgctca cagaatactt
     4981 gacgcctccg actacagaga ggtaaatgat atcctattta tagttgacgt gcttatcaca
     5041 gactacagct ctatcatata tgagatgtca ctgcttaaaa agccaatgct tttctttgct
     5101 ttcgaccaaa agtattatga agcaacaaga gatttctatg agccgtatga ggagcttgtt
     5161 cctggtaaga tcgtccacga ttttgatgag cttatgaccg ctctcagaga taaggattac
     5221 gagtttgaga aaatggaggg cttcataaag aagaacttca aatatacaga cggcaagtcc
     5281 accgacagag taatagacca gcttatcatg ggcattgagc ctgaagataa cagcagaaga
     5341 taaaaaaaga tgaatagaaa caggaggatc agtatggaca atggtatgat atcagtctca
     5401 atgatgtgcg tccatctggg acagataaag gaatatcttg acgcttttaa gcgcaacaat
     5461 atcgaatatc tccatataga cgtaatggac ggctcatttg tgccgaattt cacactttat
     5521 cctgattatg taaaacagct ccgtgatctg acggatatcc catttgattt tcatttcatg
     5581 atagaaaagc ctgaggaaag gctccagtgg tttgatatca gagagggcga ttatgcggca
     5641 gtgcattatg aaagcacaaa gcatatcgca aagtgcctgc aatatctccg ttctatcggt
     5701 gcaaagccta tcttggctat caatcccggc acacctatct gcgtggtaga ggaagccgta
     5761 gacttcatag acggtgtgct tgtcatgaca gtaaaccctg gctttgcagg acagaagctc
     5821 gtgtctaaca cggtggataa ggcaagacgt cttagaaagt ggcttgacga aaacggccac
     5881 gaagacatca tcatagaaac tgacggcaat atgtcaaacg agaactgccg caagctttac
     5941 gaagcaggtg caagaatgtt tgttgcagga acatcgagcc ttgttaagcc gcaggtagat
     6001 ggtatagatg aaaggatagc cgaaaaccgc aaaaatattt gtcctatgga ataaaaaaat
     6061 tgaaaaaaat gcagccgctc tgtatacatt gtgcagggcg gtttttacat tgtttatgag
     6121 aatacgaaaa agcggtgcag agtttccact ctgacaccgc tttgcttata tctttacttt
     6181 atttttctct ataaagtcca cattcactat catgggaata tatcactccc accgcttgca
     6241 aaaacgaata tattatcacg cttcccacga acttcattcc acgctttttc aagtctgcag
     6301 agatcctatc ggaccattcg ttggtagttt tatcgcactc atacaatatt tctccgtcag
     6361 taaacttgca aagataatca taaaagctcc cctgctcctt cacaatatcc cgaaatattc
     6421 ttgcattaga aatactcgcc ctaattttca gcttatttcg cacaatagaa ctgtcagagc
     6481 aaagctctgc gatcttctct tcgtcatatc cacagaccct atcaaggtca aattcatcat
     6541 aagccctgcg gaaattctca cgcttgttca gtatgcactc ccacgaaagc ccagcctgaa
     6601 aagactccag cacaagcatc tcaaacaaat agctgtcatc agtcctgaga acgccccatt
     6661 ccttgtcatg atattccaca tactttggat ttttaaggtc gcaccatttg cacctgttca
     6721 tataagcttc gtaccctcgt tgtcaatgtg aaattccttt acctgccagc ctttaagccc
     6781 tgcgttgtca agagaaaatt tcatcttgcc tgcgaaatat tgattgctct cgtccacaat
     6841 agccatgata gttggacctg caccgcttat gtatgccgca tatgcaccgt gagtgtacgc
     6901 aatgtcaaaa acgtccttgc agtggggtat aagcgccatt ctgtaaggct ggtgaagcct
     6961 gtcatcaact gccgttttca gattttcaaa cttgcccgta agaagcgaag ccgagaaaag
     7021 cgccgcacgg gaaaggttgt aaactgcgtc tttgtgtgaa acttccttag gcagacaagc
     7081 cctcgcttcc tcagttttca gctcaaaatc aggtattatc gccacgaatt tcagctttgt
     7141 gaaaacctcc tgctttaccc agtgtacctt tcttccgtca aataccgccg tcacaattcc
     7201 gccaagaagt gcaggcgcag tgttgtcagg gtgaccctct atctgtgccg caaggtcaac
     7261 aagatcgtcc tttgtgaggg gatctccaag cattttgttt gcccccacaa gtcctgcgat
     7321 tatgcaggca gaggacgagc caagtcccct cgccatagga atgttgcttg tctgtataag
     7381 tttaagtccc tccagctttt ttccgcatac cgcaaaaagg tctttggcag aaatgtaaat
     7441 aaggtttctc tcgtccgtag gaatgtcaag cccgtccgca gccgtaatgt ctatcttgtc
     7501 gcactcctcc atttcaatgt agttgtagta agtgagcgca agacccagtg cgtcaaaacc
     7561 ggcgccaagg ttagccgacg tggcaggtat ctgtattttt atcatggtga aaactccttt
     7621 atatgtaaaa atgtacgaat aagcgcagtt aaaattgtat tttacatcaa ttgacaatta
     7681 gtcaatgtta tgctatcctt tagttaaaga aaggggggcg agcaaatgaa tagaaaaata
     7741 attatcgctc tgtgtgcatt gtgtctctgt gcattttgtt catgcagtaa aaacgaagaa
     7801 cagaacaact cgtccgaaaa accgaccgaa acaacagcgg tgacaacagt ttcaacaacg
     7861 accaccaccc caaccacctc agagcttgtg gcagacactc cgccgccgta tatgtactat
     7921 gagggcaaaa catatacctc actttacaaa gccgaagaaa ctcttatgtc aacagacgat
     7981 aagatgaccg aaaggctgaa cggctatgag ttcgtaggaa acacccacga attttttaac
     8041 atcggtgata tgaagtcaga ctttgatgtg acttcactgc ctgataacgc caaggtctac
     8101 catgaccctg acaaagcaga tgacagcgac ccgtttataa ttgcctttga agaaaacgga
     8161 caaaccaccc tctattatat gaatttgctc agtgaataac atactgtaat aaacgcaatt
     8221 gatttctttg agggggactt attatgaaaa agaaaacagc agttattatt ctggcatttc
     8281 tggcagtatg tctttgctcg tgttcaaaaa gcaaaaatga cagcggaata gactccgaat
     8341 atgttaaaac agagagtgtt gtaactgagg agagcacaga ggaaacaaca gaggtcagag
     8401 gagctgatat gtgtccattg tatatgtatt atgagggcga tctgtatatc cctgcggtca
     8461 atcctgaaga cagactgatc tttgatacag ccgagttaaa agactataag tatgttggaa
     8521 aaacaacaca atgcattgat tgcagtggct taacagagaa ttttagggtc accattcttc
     8581 gtgataatgc aaaggtctat tataatcaga atgaaccttt ggacggcaag ccttttgctc
     8641 tgatgtctat taatgagaac ggcaatgttt ggatagcata tctcaaacta aatgatcgtg
     8701 attaaatatg taaagcggcg gagttttgat ccgccgcttc ttgtttttta tcagtatatc
     8761 cttatcttgc ttgcgatctc agcggagtgt gaaaggatag tcaggttagc gtcaatgtca
     8821 gcctctttca tgttcggcgt gataaacgca agctcgttct ctgtcctgcc cgaacgctct
     8881 atatacatga gctttccgaa catctcagaa atagaagact tgagagcctc agcattttca
     8941 gcctttatgc gaacatacat tgaaacgtcc atatccttgt atggcgcaac aaagttcttg
     9001 tcatcacaat cttcccatgt aagagcaagc actctgtgat gatgtttcag acaatcgata
     9061 atgtcgccta ctaccgctga agctgtagga agctttcctg caccctgtcc gtagaacata
     9121 acgtcgccaa cgccgtcgcc tgttacactg atagcattgt atacatcatc aacagacgca
     9181 agaagatttg actttggcac aagtctagga caaacgatag cactcactct gccgtcatca
     9241 agcttcttta cagaacctat aagctttata acatatcctg cgtttgcagc atattccaca
     9301 tcatcaagtg tgatctttgt tatgcctgag cagtgtacca tttcaggata aatgtgcttg
     9361 ccgtatgcaa gtgagccaag tatacagatc tttctgcaag cgtcctcgcc ctcaacgtca
     9421 gccgtagggt cagcctcagc gtaaccaagc ttctgagcca ttttaagagc atcgtcaaat
     9481 gacatctgct cttttatcat ctttgtaaga atgaagtttg ttgtgccgtt gaggatacct
     9541 gatatctgaa gtatctcgtt tcctgccaga cacttgttaa gcggtctgat gataggtata
     9601 ccgccgccaa cgcttgcctc gaacagatag ttgcagttgt gttcccttgc tattttaaga
     9661 agctctgctc ccttttctgc aactagcgcc ttgtttgatg taacaacgct cttgccgtgt
     9721 tcaagaagct tctttgtgta gtcataagcg aaagtcgttc cgcctactac ctcggctact
     9781 acttctacct cggcatcatt gagaatgatg ttgaagtcct ttatcatttt gtctgcaaat
     9841 ggagaatcag ggaaatccct cagatcaaga atgtacttga tgtcacattc cttgccgacc
     9901 ttttttgaga tagattcacg gtttttcata aaaagctcta ccgtaccaga gcctacaacg
     9961 ccataaccga taactgctac tttacacata aaaaatcctc attcctaata aattatatat
    10021 tgttaaaatc ttattttacc cttactctcc cgatattatc ttgacttcca caacgccgtg
    10081 aactttagaa agctcctccg taagcacgtt ggtgctaagt gcgtcatgct caaatcttac
    10141 cgagaatgtc accgtcgcca ccgagtcaat agggatattc tggtttatgg tcagaatatt
    10201 cgtgttcagc tcgtaaagcc gtgatataat ggaggaaagc actccctttt catctctcag
    10261 tatgcagtat accgaaacga tgttgtttgt gagctgttct tcataattga atacggagtc
    10321 cttgtatttg tagtatgcgc tcctcgatat ccccaccttg cggcaggctt ccgatgaggt
    10381 ggaaacttcg ccccttgccc tcatgcgctt tgcggcaagc acctttagca caatgtcagg
    10441 aagcacctca gagcttacga taacaagttt gttttgcccg ttctccaatt ttagtccacc
    10501 tccaaagtac agatgtgtgc gtgataaata caagtttacc acttttgtcc ccttttgtca
    10561 agtgacctga ccgcagatat tcaactaatt tttcaaattc agggtaaaat aaaatgtgta
    10621 ttctgactaa aatcattaca catannnnnn nnnnacatag agcaatttgt ccaaagaaaa
    10681 gtcatataga tagtgcaaaa tatataaaaa gtatggcttt gtaccccttc aaacttgcta
    10741 tttaagacaa aaaggtgtac aattaatgtt atgaaactat cctgcctgaa aggggagaat
    10801 aatatggata tgatagcaga aatactgcaa acagataaag ccgctgcagg caagcttgaa
    10861 aaagcagata tggaaagcgg cgaactgctg gagcgcacag tgaaagaagc gcaggagctg
    10921 aaagaaaacg ccctgaaaga agccgaagcc tataaagcgc agaaagccaa agaggtcaca
    10981 gagcgtataa ataccgaaag cgaaaagata acttccgcac agaacgagaa aacaacggtt
    11041 ctcgataaaa tatttgaaga aaaacatact cagtgggaga atgagatatt tgataatatc
    11101 atctctccga agtagagcaa aaattctccg tgaaaggggc tgaaaaatat gcttgacagc
    11161 tggtctgcca acggaacagt ggcgaaaata aaatatatcc acggcagaat gtttactaaa
    11221 gagaactatc atgaaatgct catgcgaaga acagtccctg aagccgcaga ctatcttgca
    11281 cattcagcca gatataaaga cgctttccgt gacgttgacc ctaataccgt ccatagaggc
    11341 tttgtggagg cgcttctcca aagagagaat ttcaatacct atatgcgcct gtgcagattt
    11401 caggagcttg ataaactgcc tttttatgac ttccttataa aaagcaagga gatagaatgt
    11461 attctgtcca tgataaacaa tataaattca gggcttgata attcttacct taacgacctt
    11521 cccggctatg tcataaaaca ttcaaaggtg agccttcttg aaatgagccg agccgagaac
    11581 tatgaacagc ttcttaaaat actaagaaat acaccgtatt ataagatact ggtgaaaata
    11641 ccgcctttgg aggacggctc agcggattat accgagtgcg agctaaggct gagaacagcc
    11701 tattataccg agcttttaaa taccgcaaag catgattttt ccgaaatgca aagcaaacag
    11761 ctctcagaaa tgataagccg agagatagat tgccttaaca taataaacgc ctaccgtatg
    11821 aaagcctttt ttggctactc ctccgaggag ataaaaaaac ggcagataag aataaaaaca
    11881 ggcacaggct cagttaaaag acttgataag tattatgagc ttgaaagccc cgaagatatg
    11941 ctcgaatggg tgaaaagatc aaagtattca aagggttgca aacagacctc tgaatatatc
    12001 gaaagtatag tccgaagttc acagtttgcc tatctttccc atatactggc tcaaagcact
    12061 gccgcaccag tttcactcta tgcctttatg aaactgtgca gtacggaagc cttgaacatc
    12121 gtgcatataa tcgaagcgat aagatataat gccgacccct cggcagttga aaaggatctt
    12181 atcatcaccg cctgagcggt aaattctcag gaaaggagat aatgtgattg gctatagaaa
    12241 aaatgtccct tgtgaatata gcaggtctta tggacgagct tgacgccacc ctgaaaaggt
    12301 gctgtgaaag cggctgtttt catatcgagc ctgcgggcaa ttctcccgac agcgcaatga
    12361 agcccctcag cgagaaaaac gagtatgacc gacctctgaa agagcttgcc cagctttccg
    12421 cacagcttgg tataactcta aaggaaactg attttaccga ctgcgacctg tcagctccgc
    12481 aggattttaa ctccctgttt gaaaagtata atatcccttt cagcgagctt aatacaaagc
    12541 ggctggagct tactcagcgt atctcagagc ttggaggggc ggtcagacaa atagaccacc
    12601 tgaaaggtat gcacagcgac tttcagcagc ttttctcaat gaagtatgtt tccgtccgta
    12661 tcggaaagct gccagtggat aacctgccga agcttgacta ttataacgag aatttcttct
    12721 ttgtcccctt tgaaacggga aaaagcttct gctggggaat gtatttcgtc cctgaacgtg
    12781 ataaacagcg tgttgatgat atcttccact caatgtattt tgaaaggata cggataccgt
    12841 cctatgtatc aggcgacgca gacgaagcct tggaaaagct gaaacaaact atagacgcag
    12901 atacagttga gaatgcaaag ataaacgagc agataaacga gcttgccgca aaagcagagc
    12961 ctgaactgca aaaagctttt tcaaaactaa ggtttatcca cgataccttt gacctgcggc
    13021 gaaatgccgc cgcactaaac gataagtttt atcttaaagg ctttatcccc gaaaaggaaa
    13081 aagaccgctt tgataagctt ttcgaggata tgcgttcagt gtcagtggtc tatctcccac
    13141 ctgacgcaga cgcctcctgc gaaccgccta cagtgcttaa aaacaacttt ttgacccgac
    13201 ctttcacaat gcttgtggaa atgtatggtc tgcctgagta taaaggctat aacccgacag
    13261 ccttcgtggc aataacctat actctgcttt tcggcataat gttcggcgac ttgggacaag
    13321 gacttcttat cgtccttggc ggacttgcct taaagaaaaa gctgggtgct aaaataagcg
    13381 gtcttgtcac acgccttggc atatcaagta tgatattcgg tactctctat ggctcgttct
    13441 tcggttatga agagctttta gaccccgtgt ttgagaatat aggacttgac ttcctgcccc
    13501 taaaggtttt caagcaaacg aactttatcc tcataactgc cgttgccata ggtgtggcac
    13561 ttaaagttat ctcaatgata ctcaatatct atatcggctt taaaaataag gattacgaaa
    13621 aagccatttt cggctgtaac ggcattgcag gacttgtgtt ctacagctcc gttgcggcag
    13681 gacttgttgg aacgctcata ttcgatgtaa aagtcatgtc cactccgttc gtcatcttcc
    13741 ttatcgtaat accattggtg tgcatattct gccgaacacc tttctctatc gccgtgaagt
    13801 ataaacagtg gcgcttgtcc gaggacgagg aaaaaatgac cgtgggcaac tttatcgttg
    13861 aaaatttctt tgaaatgttc gagttcctgc tcagctatgt ttcaaatact atgtcatttc
    13921 tccgtgtggg cggatttgtc ctctctcacg caggcatgat gctggtggta atgaccctta
    13981 tggaaatgtg ttccgccgcc gcacagccat tcgtgcttgt gcttggcaac ctgtttgtaa
    14041 tggtaatgga gggcatgata gttgccattc agataatcag acttgagttt tannnnnnnn
    14101 nnagatattc tcccgcttct atgagggcgg cggaaaacca ttcgagcctg ttgttgtaaa
    14161 atttactgca caaacggagt aacagctcat ttgcaatatt aattttagag cgttaaatcg
    14221 caaacaagcg ggacgtcgag ggcgccgtcc cctacaaatg aggtaacacc atttgtaggg
    14281 gcgaacatcg ttcggtcact cgccgtgacg ggcattttac cccgaccatt atccataccc
    14341 gtacaatgta attcgtaaaa tctaaagtaa aaataatttt tttggaggaa taaactatgc
    14401 ttatctattt cctgctccca cttatagcag tcgcacttct ttgtgtacca ttcattttca
    14461 ccgcaaagaa aataaagaac ggaagatcgc caaagggtgc ttttatcggt aatctctgta
    14521 catttgcagg cataatgctc tgcgctctta tcgtccctgt aggtaatttt gtttcagccg
    14581 caagcgagga gggcgttaag gccgcccttt caacaggcgc aggccttggc taccttgcag
    14641 cagcccttgc cgttggactt tcctgcgtag gttcaggcat agccgttgcc gcaggcgctc
    14701 cagccgctat cggtgcgacc tccgaagacc ctaagaactt tgtaaaggct cttatcttcg
    14761 ttgttcttgg cgaaggtatc gccctctatg gacttcttat cgctatcctt ataatctcaa
    14821 acgtaggcac tgcattgggc atttaacatg agattttatc ttatcagcga taatatagat
    14881 acccaaatgg gcatgagact ttcaggtatc gagggcgttg tggtgcatac ctccgaggag
    14941 gtgtcaaacg cacttgataa ggctatggaa atgaaggacg ttggcgttgt gcttatgaca
    15001 gagcttgcag tcaagcagtg cagagaaaag gtctatgact ataagctcac aagagaaacg
    15061 cccctcatag ttgaaatacc agaccgccat gccacatcaa agatatctga taccatttcc
    15121 cactatctcg cagaagcagt gggaataaag ctgtgatttt gataggagtt tagtaatgga
    15181 aaatcagacg ataaaacttg aacgcttcaa gcaggctgtc tttgaggacg ctgaaaaaca
    15241 ggcaaagctc ataaccgaat ccgctgataa acagcgtgaa aacgagcttg ctcaggcgaa
    15301 aatagaagca caaagccttg cagactcaaa aaaagccgcc gcagataaga ccgaggaggc
    15361 aagagccata agggaaatat cttcaaaaca gcttgaagct aaaagaaacg tgctttgcca
    15421 tagagaaaag cttatagaca gcgtttttga caacgtaaaa agcaggcttg cgactttcaa
    15481 agcaagcaga gattataagt cttggctaaa agaaaaagcc gaaaaatgca aacagaccta
    15541 tcccgagcat aagggcgtta tctacctctc acctgaggat atgaaatacg cctctgacct
    15601 tgcagacttt gaagtaaaaa gccgagattc catagagctt ggcggtatgc tcatggtcta
    15661 tgaggatatt ggtatagcac ttgattatac ctttgattca gcatttgagg ggcagcgttc
    15721 agcctttact gaaaaagccg agcttgtgct tgggtaataa ggagtttttt atggataaaa
    15781 tatattcggt aaacggacct gtagtaaagg ttgcagatac gaaaagcttt gccatgcagg
    15841 aaatggtcta tgtgggcaaa aagcgtctta tcggcgaggt cataagagtg cagtccgatt
    15901 ttaccaccat tcaggtctat gagggcacaa cagggcttat gcctgacgag cctgttacct
    15961 caacaggcgc accaatgtcc gcaaccctcg gtcccggtat aataaataac atcttcgacg
    16021 gcatagaaag acctctcaaa aagcttgaag aagtctcagg cgcatttatc gctgacggtg
    16081 cagacgtaag ctcgcttgat acagagaaaa agtatcccgt tacaatgaca gtgaaaacag
    16141 gggataagct ttccgcaggg gatatttatt gcacctgccc cgaaacgcca cttatcacac
    16201 ataagtgtat gctttcccct ctgtcaaaag gcggagaggt catatggact gccgaaaacg
    16261 gcgactatac aataaatgac gttgtggcaa aaataaaagc ccctaacggt gcagtagagg
    16321 agcttaccct ctgccagaaa tggcctatca gaacatcaag accatgcaaa aaccgcctgc
    16381 ctattacaaa gccgttggta acaggtcagc gtatcgttga tactattctg ccggttgcaa
    16441 agggcggaac agccgctatc cctgggggct tcggcacagg caagaccatg acccagcacc
    16501 agcttgcaaa atggtgtgat gcagatataa tcgtctatat aggctgcggc gagcgtggaa
    16561 acgaaatgac gcaggtgctt gaggagttct cagagcttat cgacccgaaa acacaaagac
    16621 cgcttaccga cagaacagtg cttatcgcaa atacctcaaa tatgcctgtc gctgcccgtg
    16681 aagcctctat ctatacgggc ataacccttg ccgagtatta ccgtgatatg ggttatcata
    16741 tcgcaattat ggcagactct acctcacgtt gggcagaagc tctccgtgag atatcaggca
    16801 gacttgaaga aatgcctgcc gaggaaggtt ttcctgccta tctgccgtca cgaatttccg
    16861 agttctatga gcgtgcaggc tatgttgaaa ctctgggcgg caaggaaggc tctgttacta
    16921 taatcggagc agtttcgccc caaggctctg acttttccga gcctgtcaca cagaatacaa
    16981 agcgttttgt acgttgcttc tgggcgcttg ataaatctct cgcctatgca agacattatc
    17041 ctgcaataaa ctggaacaca agctattccg agtatgcaga agaccttgcc gattattata
    17101 ataaaaacgt caatgagaac ttcatgaaag tcagacagga gatatcaaat atcctcgttg
    17161 aggaaaatac tcttatggag atagttaagc tcatgggtac tgacgtcctc cctgacgatc
    17221 aaaagcttct tatcgaaact gcaagagccg taagagtggg ctttttgcag caaaatgcct
    17281 atcataagga cgatacctac gtcccccttg ataagcagta ccgcatgatg aaacttatca
    17341 cagatttcta tgcccttgca aagaaagcac tttcacaggg cgtgaacgtt gataaaataa
    17401 tagactgcgg ctggatagaa aagcttatca aagtcaagta tgatataccg aacgataaaa
    17461 tagagctttt tgacgaatac gaaaagaata tgaaagccga tatggaggct ttgacaggag
    17521 gtgcaggagc gtgaacgtaa aatatatcgg tcttagcgaa ataaacggac cgctcgttgc
    17581 acttgaccat gtaagcggca tttcctatga cgaaatggca gaaataaagc ttgatgacgg
    17641 cacacgacgc cttgcaagag ttgtagaggt ggaaaaagac agagccgttt tgcaggtttt
    17701 cgagggaaca aaaggacttt cccttgttaa taccaccaca agctttcagg gcaagcctat
    17761 ggagcttgca ttgtctaccg aaatgctggg caggatattc agcggagcag gcaagcctat
    17821 cgacggtctg ggagagatct tccccgaaaa gtaccgtgac ataaacggct cgcctcttaa
    17881 ccctgtttca agaacatatc ctagaaacta tataaacaca ggaatatcct ccatagacgc
    17941 ccttactacc cttataagag gtcagaaact acctatcttc tcaggctcag gactttctca
    18001 taacaagctc gccgtgcaga tagttaggca agcgaaaatc gccgacgaaa acgggcagga
    18061 tttcgcaata gtttttggag caatgggcgt aaaaaacgac gttgcagatt atttcaaacg
    18121 ctgtttcgag gaaacaggcg ttttgcagag ggtagcaatg tttttaaacc tctccaacga
    18181 ccctatcata gagcgtatcc tcactccaag atgcgccctc acttgtgccg aatatcttgc
    18241 ctttgagcat gatatgcaca ttctcgttat cctcacagat atgacttcct atgccgaagc
    18301 cttgcgagag ttctcctcat caaaggggga gataccggga agaaaaggct atccgggcta
    18361 tctctattcc gaccttgcct ctatctatga aagagcaggc atagtaaagg gagcaaaagg
    18421 ctctgttacg cagataccta ttctcaccat gccgaatgac gatatcaccc accctgtccc
    18481 tgaccttaca ggctatataa ccgaggggca gatagttctt gacagaaacc tcgaccaaac
    18541 aggcgtttat cctgctgttt ctatcctgcc gagcctttca agacttatga aagacggcat
    18601 aggcgagggc tatacaaggg aagaccacca aatggtgtcg aatcagcttt ttgcagccta
    18661 tgcaaaggtg caggacgcaa gaagccttgc ttccgttatc ggtgaggagg agctttccga
    18721 taccgataaa gcctatctta aattcggtac gctttttgaa aagcatttta tcgaccaagg
    18781 ctttgacgtg aaccgcacca tagatgaaac tctcgaccta ggctggagct tgctttccac
    18841 cctgccgaaa tcagagcttg atcgtgttga cgaagaacat ctggagcatt attacagtgc
    18901 cgaaaaagct aaggcacttt ttgcagagga ctaaaattca gtgccgcaca aataattatg
    18961 taggggcgaa cattgttcgc ccgttattcc acaatattgc ggttatttat gatattgccg
    19021 tcactcacaa tattgttgtt tgcgattttg aaattatttg caacatcact attccaatgc
    19081 tgaataaaac ggctcacaaa gaaaaaatcg ggcgaacaat gttcgcccct actggacttt
    19141 atttatatac agtgatataa tagaaagagg gtgtgaccaa tggctgaaca gcaggtcttt
    19201 gccacaaagg gaaatcttat actttataaa aaggctctga aacttgccgc attgggctat
    19261 gagcttctgg acagaaaaag aaatatcctt ataagagagc ttatgagcct tgttgacaaa
    19321 gcaggggagc ttagaggaag cattgaggac acctacaagg aagcctacag cgcactccag
    19381 cttgcaaata tctccatggg tcttgttaca ccttatgcaa attgtatccc tgttgaaaca
    19441 ggcgttgaga taagcacaag aagcgtcatg ggcgttgagc tgccaaaggt cacccttaaa
    19501 gaacgccctc tgaaagtcac atacggcttt tctcagaccg attctcagct tgacagagcc
    19561 tatcttgcct ttgaaaaggt aaaacagacc acagcagtcc tcgcagaggt ggaaaacagt
    19621 atctacagac tgtccgtagc cataaaaaag acacagcgca gagcaaacgc cctgcaaaac
    19681 ataatcattc caagaaatga acgcatagtg aaattcatta ctgactctct tgaagaaaag
    19741 gacagagagg agttctcacg aatgaaagta ataaaggcgg caaagctaaa gtcggcaaaa
    19801 tgacatttgt aatggcgcgg cgtaatttct gcaatgttat tgtaggggcg gtcactctcc
    19861 gtgacgggca tttcacgctg tgcatttatt aatgcaacac ccttgtactg cacaaataaa
    19921 ttgtaggggc gacatgaggt cgcacgtcta gtcgccagct tgcagcatta taaaaacatg
    19981 aaaggaaaga taaaaatgga atatatcaaa aaagtattca gaagctattg gatattgtct
    20041 atattctgcg tagtgctggg cattgcgctc ataatcgacc ctaatttctt caccgccgcc
    20101 ataggctatg ttattggtgg acttttcacc gcatttggtg cagtggagct tataagatac
    20161 ttcgccatgg gcagagacga ccagagctat tcttcaagcc ttgtgagagg gatcatcctc
    20221 gccgccatag gcattttcat tatcataaga cctgatttta tcccgaaagt cattgccgtt
    20281 gtctgcggac tttatatgct cataagcggc atcgtgaaca ttcaggactc ccttaacctt
    20341 agacgtgcag gtgtagaaag ctggaacgta agctgtatct cagccgctgt taccactctc
    20401 gttggtatac ttctcctctt tgaccctctt attttaggcg aaactgcaat gatagtcctt
    20461 ggtatcgctc tgctcgtttc aggtgtgacg aatatcttcg gctgttttac cgcaggctca
    20521 aagctcaaaa aggtaaataa gcttatgaaa aaagagctca aagacagacc tgataaggat
    20581 cattatatag atatttagga tattatcgca ggggcgacct agggccgccc actttttttc
    20641 tctattttag cagtttgatg tatttcttta caaaaatatg tgcattttat tgacacggga
    20701 tgtgatttca caagtttcaa caaatatttt tgcgtcgatt tgtaaaagtc tataaaaatt
    20761 aaaaaaagcc ttgacaaatg aggtcaaaag gtatatactc ataactgtaa acagcaaagg
    20821 cgctgtgaac cacttaggtt cgcagtgtct ttttttattt ttcttagact ataatttata
    20881 ataggaggaa ttcaatatgg caaacgttcc tgaatatttc ggctcagacg tattcaatga
    20941 atcagtgatg aaagaaagac ttccaaaggc tacatacaag gctttgaaga agactatcga
    21001 caacggcgat ccacttgata tcgaggttgc aaacatcgtt gctaacgcaa tgaaggactg
    21061 ggcagttgaa aagggctgca cacattacac tcactggttc cagccaatga caggtcttac
    21121 agcagaaaag ctcgacagct ttatctcacc aactgaggac ggtcacgtta tcatggagtt
    21181 ctcaggcaag gagcttgtta agggcgaacc tgacgcttct tctttccctt caggcggatt
    21241 gagagcaacg ttcgaggcaa gaggctatac agcttgggac ccaacttcat acgcattcat
    21301 taaggaaaaa gcactttgta ttcctactgc tttctgttca tacaatggcg aagctcttga
    21361 taagaaaact ccgcttctcc gttcaatgga agctatcaat aagtcagctc tcagaattct
    21421 gaagctgttc ggctcagacg caacaagagt tacatcaaac gttggtcctg agcaggaata
    21481 cttccttgta gacagagaga tgtatgcaaa gagagaggat cttattcttg caggcagatc
    21541 actttacggc gcacctgctc caaagggtca ggaacttgaa gatcactatt tcggtactat
    21601 caagacaaga gttcaggctt acatgaaaga tcttgacgag cagctctgga aacttggtat
    21661 ctacgctaaa acaaagcata acgaggttgc tcctgctcag cacgagcttg ctcctatcta
    21721 tggcacaaca aatatcgcca ccgatcacaa ccagctcaca atggagatca tgaagagaac
    21781 agctagaaag cacggcttca agtgcctgct tcatgagaag ccattcgctg gcatcaacgg
    21841 ttccggtaag cataacaact ggtctatctc aacaaacaca ggtgtaaacc tccttgatcc
    21901 tggtaaaaca cctgctgaga acgctcagtt cctcctgttc cttacagcag ttatcaaggc
    21961 tgtaaatgat tatcaggata tcctgagagc ttcagttgct tctgctggta acgaccatag
    22021 acttggcgct aacgaagctc ctccggctat tatttctatc ttccttggcg acgagcttac
    22081 aggcgttctc gatgctatcg taaacggcgt taagtatgag gacaagagag ttgagatgga
    22141 gatcggtgtt gacgttctgc cacacttccc taaggataca acagatcgta acagaacttc
    22201 accatttgca ttcacaggca ataagtttga gttcagaatg cctggttcaa agctttcagt
    22261 agcaggtcct aacacagttc ttaacactat cgttgctgac gttctcgatc agtttgctga
    22321 cgagcttgaa aaggctgaca acttccagga cgctcttgac gatcttatca agagaactat
    22381 caaggaaaat cagaacatca tcttcaacgg caatggttat tcagacgagt ggcctgttga
    22441 agctgaaaag agaggacttc ttaaccttaa gtcaactcct gaagctgttc ctgcattcct
    22501 tgcacaaaag aatgttgacg tattctctaa gtacggtgtt tatacagaag ctgagcttca
    22561 gtcaagagtt gagatcctcc tcgacgagta ttgcaagacg ctcaatatcg aggctcttac
    22621 aatgatcgac atggctaaga aggagatcct tcctgctgca gctaagtata tcaaggatat
    22681 cgcaaagact gctgagcttg caaagagctg cggtgctgaa actgtatttg aggaagagac
    22741 cgttaaggaa atttcagctc tcgtaactga gatgtacaag gcacttggca cactggaagc
    22801 tgatgtacag aaagtacacg ctatcgagga cacacaggaa atggcaaact tcttccacga
    22861 tactatcttt gcagatatgg gcgctctcag agttccggct gataagatcg aaacactggt
    22921 aggcaaggag tactggcctt atccaacata cagcgacctg ctgttctacg ttaagtaagg
    22981 gacaattcaa attttcaata taacacggac tttataacaa actaaccaca gatctactat
    23041 tttgttcccc tacagctaca ataatatacg gaatgcacaa tgaagtgtat gacagaccga
    23101 gccgaatggc tttggtcgtc atacactttt ttgtatttta ggaggaaaaa gatatgacaa
    23161 acactgtaac agtattggct gaaagcacag gcttcagtgc cgtcaatact atatgggtac
    23221 tgttgggagc tgcacttgta ttctttatgc aggcaggctt cgcaatggtt gaaacgggct
    23281 tcacccgtgc gaaaaacgca ggtaacataa tcatgaaaaa cctgatggac ttctgtatcg
    23341 gaactccgct gttttggctc tttggtttcg gtataatgtt tggcgacgga aagtttatcg
    23401 gagcttataa gggaatagct gattctacat atgcctcagc tgctccagac ggagtgcctt
    23461 tctttgcttt ccttatcttc cagacagttt tctgtgcaac agcagcaact atcgtttctg
    23521 gagctatggc agaaagaaca aagttcattt cttattgtat ctattcagca attataagcc
    23581 ttctggtcta tcctatcgag gctcactgga tctggggcgg cggctggctt gcagacctcg
    23641 gcttccatga tttcgcaggt tcaacttgcg tacatatggc aggcggcgtt gcagcattcg
    23701 ttggcgcagc tatccttggt cctcgtatcg gtaagtatga caagaacggc aaggcaaagg
    23761 ctatcccagg tcactcgctt acacttggcg cacttggctg ctttatcctt tggttctgct
    23821 ggttcggttt caatggtgct tcaacaactt caatggaagg cgatgctatc ccgcttgcag
    23881 gtaaagtttt cgtaacaaca aaccttgcag cagcagttgc aactatcaca acaatgattt
    23941 ttacttggat caagtataag aagcctgatg tttcaatgac acttaacgct tcacttgcag
    24001 gtttggtagc tatcacagct ccatgcgaca cagtttcacc agtatcagca gctataatcg
    24061 gtatcgttgc aggtatcctc gtagttgtag gtgtagagtt tatagatcag gtactcaaga
    24121 tagatgaccc agttggtgct tgcggagttc atatgctcaa cggtatgtgg ggtactattg
    24181 cagtgggtct tttctcagac ggtacttgcg gtgaaaaggg actcttcaca ggcggtggag
    24241 cacatctcct tggtatccag cttctcggta tggtatcagt aatcgcttgg gtagctatca
    24301 caatgactat cgtattccag atcatcaagc acacgatcgg tcttagagct tccgagcagg
    24361 aagagatcct tggtcttgac gttacagagc atggtcttgt atcttcttac gctgacttcg
    24421 caccagcaat gtcacaggca atgctcagct atacaaagga cgacgcaaag agcgttgtaa
    24481 aggtagacaa gtcaacagag cctgtagttc ctgttgagaa ggctatcaag gtagaatctt
    24541 atgcaacaga cgcaccgtct gctgacggca cacctaagat gacaaaggtc gctatcatct
    24601 gcaagcagca gaagctcaac gatcttatca cagctttgga ggacatcgac gtaacaggtg
    24661 ttacagtaac gaacgttctt ggctgcggaa tgcagaaagg acagcctgag tattaccgtg
    24721 gcgcagttgt tgaaacaaat ctgctgccaa aggtaaaggt cgaggtcgtt atttgcaagg
    24781 taccaacaag agcagttata gatgctgcaa aggcagccct ttatacaggt catatcggag
    24841 atggaaagat ctttgtatac aacattgaga acgtagtaaa gatcagaacg aatgaagagg
    24901 gttatgacgc acttcaggac gattaagcct taaagatgac aggtgactac ccaataaaat
    24961 tgcccccatc ccacttataa attatagctt ggaacccgtg gtcgagtaat tcggctgcgg
    25021 gtttcaagtt ttgtggagaa gttcaaggca ttatgaatag atagacaaaa gggacattgc
    25081 cgaacgttgg caatgtccct tcgtaaatta tcagaaaagt gatagctgct cagataaata
    25141 aaaagcgtgt tccatatgtt gctttgcctc tgcttcaagt atttcgacat ttgagagtgg
    25201 aaggttcttg aaacgaagct gatagaccat atcaaagaag ctcgttacag ccgcagttcc
    25261 aagatgcgta agacagatca gctgtatatg gaaatgtctt gctatgcgga acataggctc
    25321 aagaaggtga ggcgaagaga tcggtccgaa tggattatca aggatcagta cgccgcttgt
    25381 tgaatttaga ttgtttgtaa ggcttcttgt gtaattcata acggatacga tcagagaaaa
    25441 taaaacaaca aattgttctc cgccagagtt tgctttcaga gcgtcctgcc atgaacgata
    25501 gcggcagttc tgaatatttt tatcgatctt aaatacattg ataggactgt tctcattgcc
    25561 aatgtagcaa ttcaagaggc gtctgatatc aagatgatcg ttatgagttt tcgttgctgt
    25621 tgtattttca gacagatgct tttggacttg ttcagttatg aaatgatcga tccttttata
    25681 aggctgatcg ctgtctggat caatctcagg caaaactatc tgtatcatat gtttttttac
    25741 gttaccgatc tgtacgaccg atctaccaga cagtgttttt agattatcat ataaacgtcc
    25801 ggcacgctgt ttgcaatgat ctacaagttg ctttctgctg tcttcaacat ctttaagctg
    25861 aacggcaagc tgagcgatac gatcctgata acgcttgata tcgctttctg ttcgttcata
    25921 aagtgtaaag tatttatctc ccttgatatg atcgtcgtcg atcactgata gaatgccgtc
    25981 cagtgttccc ataaacaaag tatgtgtatt tctgaatggc tcaagttcac ggctatggta
    26041 tttctttgct ttgccctctg cttgtgtcag ctgatcgatg caatgcttta ttgtatcgat
    26101 caagttatca gtatcctgcg ggatctctga tattttagga gtatactctg taaaatcaag
    26161 cttgagatat tttttgaatc tatcaatgta tatccatttt atattcaaac cgttcaattt
    26221 ctttttgcat tcttcacttt ctcgctcagc tgcttctaat ttattttcac attcttttat
    26281 acggcgttca aaatcagaac caacctcaga acgtggcaaa agctctgcat tgagttctgc
    26341 tagctttttc ttagcatgtt ccagatcatt tttcactttg gtatgatagt tcgatgcttg
    26401 agaatgttgc tcataagcct gcgagtttgc ttcttttgcc agatcaagct gttgttcagt
    26461 gtcattatag gcgttggcag aatattctgt gcttttgtat tctgttggtt ctatatggaa
    26521 tcttttgata tcattttctt tatgaacgat gctttcttgc acctgttcca gtcgcttctg
    26581 cagcgttttg cgattagctg aattattctc cagataaagt cggtattcct cgagcatgac
    26641 cgaatgatcc tctgtaagta tttctgcttt cggtgtgtct ttcagctcat gcgacagttt
    26701 ttgatgatcg gtcagcttat tctggaggtc acggagaaga atatcattat ccgttatact
    26761 cttattcagt tgttcttttt tctgatgcat gccatctatt tgttcagaga tctctctgca
    26821 aatagttttc aattctatga tcttttcgct aacgctctca tattgtgaga tgatcttaca
    26881 aatacgctca aattcggatc gtttattttc aatatgggat tttgatattt gacttttttc
    26941 gcttatttga cgattttcct ttgctcgtgc tttgatctcg ctcttttgct tctcaagaac
    27001 cgagagctta gtttgtatct cctgaagctt ttctttaagc ttccctatgc ctgcctgtaa
    27061 ttgctgctca tgatctgcgg cgtatgtaaa ttgtgatagt atgctgcgtt ctgattccca
    27121 ttccaatatc tgatgctgta gattatcttt atctgcttgt gtttcgccaa gtcttttctg
    27181 tatattttct ttataagtaa atgcgtcctg aaaatattct ttatcatatg cagaaataaa
    27241 ccgttcaggc tcgttccaat ctccatcagc catatccgca agttcagagc gtgaatatac
    27301 tggaacaaca gccgataacc aagaatctgg cgattgtgac agcaatttga ccttctcctt
    27361 ttcagaatca acgatcacag aataagcaac gagaggattg acggtaagga tatcttctcg
    27421 tatttttgca ttttgtaaat ttaggtactg ttcgcctgtt tgataagcga tacctgattc
    27481 ctgcaaaaag cttacagctg tttttgagag gtgaagctga cccgcattta tacatcgtag
    27541 ctgctcgctt agcaattcag cacagcgttc cgtttgccga agctgttctc ttagctttgc
    27601 aagtatttgc tcaaggcttg ataacggctc atcagtaaat aatctgtctt tagatattga
    27661 taaatgctct aagactttaa taactgcatc atatgcttgc tgatattctt tcaattctgc
    27721 ttcctgctgc tttaatttgg ctttacattc ctcttgcgtt cggtgaagct cgcttatctg
    27781 attatcctga gcgtcacgtt cctgttcaag ccggctctcc tcaagccggc actttttgat
    27841 attctcctca gcaagcctga gctgctcatc gtagtccgca cgaatgctct cagcgtcttt
    27901 cttagaaaag cttccgtcaa gcataatagt catatgcaaa gcaagcgttg ccagagcctt
    27961 gttgatctga tcaagcaaac tctttttttg accaatatcc tgatttaagc tgtcattctc
    28021 gtgatcatgc tgctttttaa ggcgtgagat ctcttccttt atggaatcca aagcttcaga
    28081 cagttctttt ttagttgctt ccttctgctt taatttgccc tcagtctgcc ttatcagctc
    28141 tgttgttagc aaagacagtg aatattttaa agaa
//
LOCUS       DAWEMR010000009        27942 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_699_length_27942_cov_11.341951, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000009 DAWEMR010000000
VERSION     DAWEMR010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 27942)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27942)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27942
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_699_length_27942_cov_11.341951"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    970..979
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1456..1465
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4650..4659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14453..14462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8018 a         6361 c         5797 g         7726 t
ORIGIN      
        1 actattccat aatactttat tataacacgc ttgcccgaca ttgtcaattt ctccgcaaag
       61 aaaaaaaggc agacacaacg tgcctgcccc aaaatacata ttattctgcg ctgatatatg
      121 ccgttacagc gtcatcagag ctgtcagaag ttgttccctc tggataagag ctgaatgtga
      181 acttgtaatc cttgtagccc tttaccataa ggaaaaggtt tacatacttg tatgtatttc
      241 caacaaaata ttcgttcttt ttctgtgtaa gaagctcagt agttccgccc atgccgatat
      301 aagtagaagc tccgtcagca gaaggtattc ctccgccgcc cttgttggaa gcagaatagt
      361 ttacatcagg agagattatg ccccattcat cgctaggaaa atcctcagga acatagacct
      421 catattccat aacgcaaagc tcacagtcat caggtatttt cagctcgctc tcgtctatct
      481 tagaaaaatc actgccaaca gagttgttga gctcaatagc cttgtttaca taatctgcat
      541 cctcactttt gctaactacc tttgttattc ttgtataaat agtgtggtag atcttgtctg
      601 tagcacagta tctggtagtc tttatccact gaccgatagg cgcaagattt tcctcgcttg
      661 tttcagaagg ctcaatagca ctagccgcaa tatcgttgct gtcaacgctt gcaggcttgt
      721 tttcgtcagc agtgcttgtg tcagccacct cagcctgtga tgattccgtt accgcaggag
      781 cagttgttgt ggtagttgtc gttgctgttg catcttctga tgagtcagca gcactgcttt
      841 ccgtctgcac agccgcagca gagctttcgt tatcactgtc ggaagaccca ctgtcagaac
      901 agcctgccat gccaaaagcc attaccactg ccaataaaag agcagatatt tttttcataa
      961 tgttacctcn nnnnnnnnna tttctttaat aaatttattt tacaacattc tcctatgcaa
     1021 gtcaacaata ttttcgacat ttataaatat tttgttaaat acccacaaac agccatagtg
     1081 tttttacaac ataatgtagt aagaaaagca caacataatg ttgtatgata tattaaatat
     1141 agactatcag aaaggaatgg agcgccgcat ggtttttcaa agaatacgag atatgcgaga
     1201 agatgcagac cttaaacaaa acgatatggc aaaaatactc cattgctcac agcaagctta
     1261 cagcaactac gaactaggac agcgtgatat tcccacggat atcctcatag ccctagctga
     1321 atatcacaaa acaaatgtag actatcttct gggactaacc aaaaacaaga cctactataa
     1381 agatatgtaa agcggaaaga acatctcttt ccgttttttt tgcaacccat ttcatacaca
     1441 tttatacaat cctctnnnnn nnnnnaatga agttgtattc acgatttaat gcggaaggag
     1501 cgttaaaaat gcttaaaaaa ggcgacgtca tatcatatgg tacaactgga gtgtgcaaag
     1561 tcgagggcga gtgcgaacag accgtcaagg gtcagaaaaa gcgctacata gtgctaaagc
     1621 ctgtccacca aatcaattcc accttgtatg tgccagtaga taatgacgag cttaaaacaa
     1681 agttcaaacc tctgctcaca ggcgatgaga tagaggagat cataaaagaa atgcccgtgg
     1741 agagctccct ttggaaagaa agcagcagcg agcgtgccga gctttattca caggtacttg
     1801 aaagcggtga cagaaagcga cttgcacagc ttgtgagaac attgtatctc agacagaaac
     1861 atcttattgc gaagggcaga catctccatt cattggacga acgttttttc aagaatgcgg
     1921 aaaatctgct ctttgacgag ttttcagccg tcctgggcat atcccaagac gaggtcttcg
     1981 actttataga aacccgcctg agcaaagcct aaatatatac aaatgacaca aataatatcg
     2041 gctaataaaa aaaggacaaa ccacagtggc ttgtcctttt tgttttcata aatgatactc
     2101 gattattcct tgtcaacgtc ctcaacaggc tcctcgatag cgtcgagctg aacttcaacg
     2161 tcagcgtcct cctcagttgc atcctcgtcc tcagcaggct cttcagcctt ttcctcagac
     2221 ttctcagcgt cctctgcctt gtcagcttca tcagcctcgt cagaacactc gtcgcagccg
     2281 cagcaatcgt cgaaatcctc gtcgagatct tcgtaatcgt aattttcaag ctccttgtgc
     2341 tttttaaatg caaaaatagc agcagctcct gcagcgatcg caccgagtgc agctataact
     2401 ttcaatccgc ttgccataat aaaacgctcc ttttttgaat attccactct gaactgcgtt
     2461 cagactatct acagtatagc atatttgtta taaaaaatca agacgtttac aaaaaatact
     2521 gattttttat acaacctgct caaaaaatct cagtaagctt tgacaaattg cccactgcta
     2581 catatttccc gtaaggctca ttatcacgct aagagccgcc agtggagctg tctcgcaccg
     2641 cagtatccta ggtccaagac tcaccacagt gacccctgcc tgactacagc tctccgcctc
     2701 atggctctcg aaaccgccct cacagccgat aaacaagcca atagtctttt cctcgccctc
     2761 actaacaggg gcaatgcctg tctcgctaag gctcacgccg cccttttcgt aacacataaa
     2821 gctctcatcg cactctttaa gttcactaag gcactcctca aagctgataa aattcgtcac
     2881 ctcaggcaca tatcccctgc ccgactgttt cgccgcttcc aaagctatct tattgtatct
     2941 ctccacacgc ttttcatagc cgctttttgc aggtcgtgaa acgcacctcg ccgtaataac
     3001 aggcactatc ttgtacactc caagctccac agccttttgc actataagat ccattttatc
     3061 agacttaggc aacgcctgat aaagcacaac attcacagta ggctcgtttt cgcttttctc
     3121 agcagaaagc acatcgcaaa ccacttcctt atcagaaatg gacagaattt ttgctctgta
     3181 ttccaccctg tctgcacata ccgtaatgac atcgcccaac ctcatacgca aacttctgcc
     3241 gatatgataa gcatcattac ccctgataga aatcgtattc cccgatacgc tgtcaacaaa
     3301 aaatctaggc atattcccaa ccagtgtcaa gcactgtcct ccaatctcaa atcacgattt
     3361 tacgcacttt cacgacgctt tcgtcgcttc ttggtatagt ataccacacc ttcaaaaatt
     3421 gttcaacaaa gttcacataa ctaacacaaa actttcatac gcagtacata cgggcagggt
     3481 attatatagt tgtcgcaagg gacaagacct accgagggaa gttccaagag cggcagttga
     3541 ttttggtatg taggctttta ccccaagcct tcatatatac agcttgtaga ttaatttcta
     3601 ctagcttctc ccctataatt ttttattttt tggcacccac agttttctgt gggtgttttt
     3661 ttgtcctgtc gaggatatcg caggaaaagt ttgtaaaaca aactacaaaa gcggtgcaga
     3721 atttacactc cgacaccgct ttgtgattga tttttcattt gcaattcttc tattgctttc
     3781 tacgccgctt agtaggcttg cgcctattga ggctctgccc atttcttgtg agcgaagtcg
     3841 aacaagaaat ttactccgct agggactctg tccctagacc ctgcaatggg tttcacccct
     3901 ggaccttttg ctcgcttcgc tcggtgagcc ttttactttg cattggcttt tcaaccaaat
     3961 tacgcaaaca gttcccctgc cgtatcttta taatactccg cctgtgccgc ccacattgct
     4021 gtatatattc cgcctttggc gataagcttt tcatggctgc cacgctctat tatgtggcca
     4081 ttgtcaagaa ctgttatatc atcgcagaaa cggcaggagg acagacgatg tgaaatgtag
     4141 atagccgttc ttgttccaac aatgctgtta aattttgtgt agatgtcata ttcagatatc
     4201 gggtcaagcg ccgctgtagg ttcgtcaagg actataaacg gcgagccttt gtatatcgcc
     4261 cttgcaagac acagcttctg tgcctctccg cccgaaattt ccacaccgtt ctcatcaaaa
     4321 tccttgtaaa gacaggtttc aatgccgttc tccatagttt taagacggtc gccaagtcca
     4381 gcacggataa cacagtctgt cacacgctcg gggtcatatt ccgtatcggc agcaacattc
     4441 tccgcaaggc tgaacgaaaa cagcttgctg tcctgaaaca ccaccgagaa aagctccata
     4501 tactcttcgt atttatattt tctgatatca attccgttga gcgttatctc gccctcggtg
     4561 ggatcgtaca gcctgcaaag cagcttaaca agcgtcgatt ttccgcagcc gttcttgccc
     4621 acaagagcca ttttctcgcc aattcgccan nnnnnnnnnt agtgcccttg tacttttcgt
     4681 cgggaatatc aagaaagtca aaatatttct tgcagaaagt cggcgttatt ttcagcagtc
     4741 cgtaaaagtc tgaaagatct gacacgcctg cggatatctt ttcaaagtac agtgcaaaag
     4801 ctatcacctc gcctggagat aacattccgc ttgccgccct catgaccgca aagccgtaca
     4861 taagtgcctt tgtgaggata ttgaccacct gacaaagaaa attcggcagc atagctgact
     4921 tataagcttt gaaccaaccc tctgctatct catctcgtgc tttatcaagc tctttatcaa
     4981 taagattttg ctgggaatat attcttatct ctttgccgtt tcgataattg cggagaatgt
     5041 aattactgaa aaagccatct gcacggtttg cctgaatgac ctttttgtca ttttgtaact
     5101 tttcaaaacc aagtatcgct ttagggttta tgaccaaaga tttgagcatt tcaagcaaaa
     5161 cagttattat aactaccgca agaaagcccc atatagactg aatgaaaccg ctgaagcctg
     5221 ctgtggtgac aggctttaca aaaaccattg gtatgataag tataacacct gtgattatca
     5281 aaaatagtcc gccgatagaa tatatgacat atttgcacaa tgccggtatg cctgcgttat
     5341 tattctcgac ctgattatct gcgtttgaaa atctgttttg tgtgtctgta ttttcaaggc
     5401 ggacaaaatc cattgtcatt actttctgcc aaagattttt atgcagattg gtacttctgg
     5461 caaaagtctg agaacttgaa cgcctaaaaa gaaacatctt tgtcataaat atcacagcct
     5521 gcacgccaac aactatcgcc gcataagaaa gcagcgttcc aacaggtttt gactgcgtaa
     5581 tcccgtcgat aacaagggca gacagatata taaagacaaa ttccgtggca agccccaaca
     5641 cattctcaat agtatctcta atgagataac cgctgtcggc gacccttact gctttcagcg
     5701 ttcgctttat tatctcacgt ttttcctttg cggaaagctt ttcttcgttg ttattattat
     5761 ttttactcat ttatctgcac cccccttatc attataatag gaactttgaa gctcaaaaag
     5821 ctcggcgtat tttccgccct gcgccataag ctgagcgtga gtgcctgttt ctgttaaagt
     5881 gccgttttct atcattatta tcctgtcgca gaaccttgtt gaggcaaggc ggtgtgatat
     5941 aaaaacgctt gatttgcctt tagtgaaatc ggcgtatttc aaatacatct cctgctctgc
     6001 gatagggtca agggcggcgg tgggctcgtc aagaagaagt atcggtgcgt ccttatacaa
     6061 tgcccttgca agagcaagtt tttgcgtctg accacctgaa agctcggtgg cattttcaaa
     6121 aactgacttc acaagccgtg tgttttcctt ttcgggcagg ctcataactt tatcgtaaag
     6181 ccctgccttt tttaggcagt cataaactct ttcacggtca aaattatctg catctgcacc
     6241 tgcgatattt tcagcgatag tcaccgcaag cagggcgata tcctgaaaaa cagcgccgaa
     6301 aagtgtgaaa tattcatcac gattatagct gttcacaggc tttccgttga gaagtatctc
     6361 gccctctgta gggtcataaa gtccgcacat aagctttatg agagtggtct ttcctgcgcc
     6421 gttaagtccc acaagtgcaa gcttttcgcc cttatgcaaa gtaaatgaaa tattttggat
     6481 agtcggcttt tcagcacctg aatatttgta gctgacgttc ctaaattcaa gctcgcactc
     6541 ccctgtcgga agtttttcgc ccttgccacg atttgtttta tcgggcatat cgagatattg
     6601 tcttgcatag gaagtattgt ttgatagcca tttgaaacct gagaacttgt caaaagcaac
     6661 tctgacagaa tgaccgaaat tccatatgga attgaaatac agcacaaagt cgcctgcgcc
     6721 gatattgcct ttaactacca tataaataac ataagcatag gcggcaagat ctgcaagctg
     6781 caagatgata gttgacacca cattgtggcg aaatgtccat tcgtcctgct gttcatacca
     6841 attcaagcga tttgcatatg agcggtcgaa aatctttgtg aaccatttgt ccattccgaa
     6901 aatgcgtaca tcttttgccg atgaaaaatc tctgcctgcg tcgccgatat aatcaagctc
     6961 acgatcatac tgctgccaat tatctgccat gttccatatc catttcattt tgtgccgctc
     7021 aagataatag accgtaatgg cgggaactcc gacgataagt atcattatag ggtcaagcat
     7081 tgaaagtata ccgccatatg ctgcgagctg tatcagcgcc ataagaaagc cttcaatagt
     7141 atcaaccgtc tgataggtta tgtagtctga acctgtccga gctttttgca ttaggttgtt
     7201 gttatcacaa ctttcgttgt tttcataatc agttgacata tgcttgcgga gcatttttct
     7261 cgtcatatgg tgaacaaact taaagtagcc cacatatcca cggtaatctt tcgtttcatt
     7321 ttcaatgtac tttgacataa attcgccgac tataagcagc agacatatga cagcaagcct
     7381 tatgcgtgat gatgttccca gagcaagctc aacaacatat ttgcttgtga acgtatcaaa
     7441 aagcgtggcg gcaacgtttg aaaatatatg caatacgatc aacatcagca ttatcggctg
     7501 gcttttccaa agctcataaa gggtataccg catattttca aaggtcttgt actttatctt
     7561 actttgctct ttgttgtact cgtcacgcgc tttctggcgt ttttcttttg tatactttgc
     7621 aatttttaca cgtttcatat cctcacctca cattcatcat acagcaaaaa agaccgcccg
     7681 tgttgatttc tgcacgaacg gtcaaaatac ggcacgaatg gcaatatagt ttacagttta
     7741 tggttaatag tgaatagtga gtagttagta gttggatttt gttgatttta ttattgatgt
     7801 gagtatcttt attagctcga tgcaatcgga actgatgctt tcatatgtca cctcataact
     7861 attaactatt caccatcaac tattaactat tgagcggcac cattatttcg gtggcaaaca
     7921 cgccctcttc attctgacga ttaacatatc cgccgtacct tttcgcaact ctgtcgattc
     7981 ggaataagcc gtagccgtgt tcaccctctt tggtggagag atatgtattt ttcgacttgc
     8041 gcactttgcc tgcggagttc tgcacagaaa gatacaactg tcctttcagc ttgccgatat
     8101 acacacgcat aaatcgttcc tcttcaggaa gcgtgccaca ggcttcgact gcgttatcca
     8161 gtatatttcc aagcatagaa caaagctcaa catcgctcat aggcagtttg tcgggaacat
     8221 tcgcttttac gttaagcttt atgttcattt tctccgcaac attcagcttg ctgttgagga
     8281 tagcatccgc cataacattg cccgtcttta ttacagtatc cacagtgtca agatcgtccg
     8341 caagctcgtt gagatagtca gacagcttat cataattgcc ctctgagagc tgaattttca
     8401 tattgttgat atggttgcgg taatcgtgcc gccagccacg cacatgacgg tacatattct
     8461 gtatctcacg cacctgcttt tcgataagct cgctctgaaa gcgctctatt cggcggtcta
     8521 tcatgttata tagggtgcgg cggattagga ataatcctac aacgcagagt gctgaaaata
     8581 agattaccgc acctattatg attgctattt tcatattagt cctcacaatc acttataggt
     8641 tcaaaccctt gacttattag acattcatta agtagttcaa aaaacatatt aacagcttta
     8701 tcttcgtcac cttcagtttc gtaaatcaat gaacaataat tcaatacggt cttgttatga
     8761 ccaagcaact tgacagcaac aaaatcgtta aagccctcag gcaaaatatg gtgtttttca
     8821 tcattgaaat gcaatttagc tgcacagttg taaccgttga aaaagttttg taagaactcc
     8881 aatgacattt taccaatata cataccagga cgtcgtttaa tattagctaa taattttctt
     8941 tcgactatat ttatatctgt tattgcgttt tccataactt tatcctttcg tatagaactg
     9001 aataaatttc tcatttacat ctttataaag tcgccgagaa agcggaataa tcgtaccgtt
     9061 atcaagagca atttccgtct tgccgatact ccgcataaac cgcagattta cgatgtacga
     9121 gcggtgagga ctcacaaatc cattcagcct cattgcaaaa aatctgctta tactcaccat
     9181 gcagtctatt atcctatcgt ctgataagtg aactgctgtt tttctgccga aagcttcaat
     9241 gtaagttatt ctgtcggccg atatatgtgt cgctccgttg gtggtttcaa taatcagctc
     9301 gtcagctttg acggaacgtt tctctacata tttatcgaga acagcgaaca gcttttcctt
     9361 agctacgggc tttagcaagt aatgcagggc ttcaacgtca tacccctcag caatgtaatc
     9421 ggaatagccc gtaatgaaca ctatcgggag catatcccca ttggaacgaa ggtgcttggc
     9481 aagctccata ccattgagcc tatccatttc aatatccagc aggataaggt cgcaaggctt
     9541 gtcgggatat gaaaacagga agctttctgc acttatgtat tcgtcaatat caagcgcaaa
     9601 aggcttgtcg gcagaccatt ctgcgagaag ccgacgcatt tcttttattt gattttcgtt
     9661 atcgtcgcat attgctattg tcagtggcat ttagttcacc ttaaaaagtt ttgatttttt
     9721 taattatatc atatcagtta tttttttgca acaaaacata ttcaataaat ggaaatctaa
     9781 gcaacgcaaa aaagcggaac gccccacaca gaacattccg cttaaatcaa actattctat
     9841 ttagttatat tcagggagcg attatcttgc accctcagca aagccactgt caacaaggtc
     9901 aacaagaccc tttactgctg cttcctcatc tggaccgtct gcaatgatcc tgatagttgt
     9961 gccgcctacg atgccaagtg aaaggattcc aagaaggctc tttgcgttta ctcttctctc
    10021 ctctttctct acccagatag atgacttgaa ttcatttgcc ttctggatga aaaatgttgc
    10081 agggcgtgcg tgaagtccta cctgattctg tactactacg tcttttacga acatactgat
    10141 ctctcccatt cattataatg ctgttaaact ccctaattaa gggacttttt caagataccc
    10201 tcaggctatt tgatatattt attatactcg acaagtctgc cttagtcaac gcaaatttta
    10261 gacaaacaag cgaaaaaccc gcaattttca gcacaatgct ctaaactttg caacttttgc
    10321 gattaaactt gtgatatatt cacaacacag attttacatt atatcacttt gtacaaattc
    10381 atttaacaat tatgtgaaaa gtgttgaaaa ccaatgtcac tttttattct ctttttcttc
    10441 taatttgtgt ataatagcca aatctttttc cgtcagcgga cttctttcca acagctcagg
    10501 acgcttcaaa taggtggctt ttacagcctc ctcacggcgc catttcgcta tctcagcatg
    10561 attgcccgat agaagcactt taggcacttc cctgtcgtgc cacacctcag gacgtgtgta
    10621 ctgtggatat tccagcagac cattataatg gctctcgtcc tcatagcaat ccgcctgcga
    10681 aagcgttccc tccagcattc tcacaacgca gtccaccagc accaacgcag gaagctctcc
    10741 gccggtaagc acataatccc ccacggatat ttcttcatca actatttcgt caatgaccct
    10801 gctgtccact ccctcataat gaccgcataa tataaatatg ctgtcaaggc gagaaagttc
    10861 ctttgcacgc ttctgggtca gcgtttttcc ctgtggggac atatagataa cgtgaggctt
    10921 tgacttgccc tcagtcaccg acttgtagca gttgtaaata ggctcgcact gcatgagcat
    10981 accctgtctt tcgctgtaag gggtatcgtc tactctccgg tgcttgtcct gagtgtagtc
    11041 cctgatatta tgacacgtta ccgtgaaaat attcttagcc cttgccctgc ctactacgct
    11101 ttcagaaaga taattctcaa gcatttcagg aaaaagcgtt gcaatatcta tgttcaaatc
    11161 aattcctcca tatcatgtca aaagactggt aagcccagtt ttccgcagca ctgttcaaat
    11221 gctcctgcac agagccaccg cttttttcat agtatcctat gccctgcaca acaccgatag
    11281 tcaccacaat agcgattatt atcaccacaa ataaactcca caaagcagac ctgcgcttct
    11341 tcggcttgtt gccctcgtta ttatagtata ccctcagact tgtgagcata ttgtcaagct
    11401 ccataaaact cagcccctcg cccttatatg tgttggcaag cctgttgaat ccgctctctt
    11461 ttctcagcct ttttatgagc catttttcct cagccctggg acttttcagc cgtgggcttt
    11521 catctatgta tgccgtaaag actttttcga tacatttcag cgacttttca cggctgttga
    11581 gctttggaaa tatcaggctg aacattccgt cagaataatc tctggcaagc ctttccaagt
    11641 cctcgtattt gcagaacttc attaatcaaa aagcccctcc agcggagtta tcttcatcac
    11701 gccaccctcg atatcagttt cagccacaac ttcaggtatc gcagggataa gcttaacgtt
    11761 tcccttttcg tcagcgatgt ggtaaacatc gtttgcgcct gtctgactta cgtctgttat
    11821 cttgccgtag ctttcaccgc tgtcagcgtc gaccacttca agacctataa gatcctgaac
    11881 aaaataacag ccctcatcaa gctccacatc gtcacggtcg atataaagca ccctgttgcg
    11941 aagcttctga gcctcctcca cagtgtcaac gcccttgact ttcattacaa cgatattctt
    12001 tgcaactctc gaacgctcta tctccacagg agtaccactt ttataatata gtgtgtcaaa
    12061 tccgcagaga aattcaggtc catcacacca tggctgtact tttatctcgc cctttatgcc
    12121 gaacaccgat actatctttc ctgcttcaag atattttttt acgtccattt tgattacctc
    12181 ccgataacaa atcttacagc tttcttttta tagattgtaa catatttttt taccctttgc
    12241 aacgctttaa ctacgctgtt atgcacatag ctcaggatat ttacaaaaag tttattaatt
    12301 acaattcggt tactcaaaac gaactttacg cccctctttt gtacaaaatc accatgaaaa
    12361 cctgtgattt aacaaaaaat tcacacataa gagagcaaaa tgtgttacaa tttgtaatat
    12421 atcagaccgc agaccagtgc ttgaaactgc ggtataaaag tttttttgca tattttcact
    12481 ataaaacact tgcaaaacat atcgtttttt gttataataa caatgtgcag acatctgccc
    12541 ttatggggta attatgccca tggcagaact taggtcgaaa atttttgcaa gtagagggga
    12601 ataacttatg tcaaacagat tatttcagag cgttgttcat cagatgcgtg acactatcga
    12661 ctgcgtcata ggcgtaatag atgagaacgc aactatcatt gcctgctctg agctttcaca
    12721 ggtcggaacg actaacgaat ttgtttcgtt agatctgggc gattcacacg acatttttgt
    12781 tagagacggt tatacctata agccattcgg tgctcatatg aaacctgact atgctgtttt
    12841 cgttgaagga acagacgagg tggctgcaaa gtatgcaagc atacttgcca ttactctttc
    12901 cagcataaag cagtattatg acgaaaaata cgacagaaac aatttcatca agaacgtagt
    12961 gcttgacaac gtgctgccgg gcgacgttca tgttaaagct agagagctgc atttcagcgc
    13021 tgacatttca agagtcgtgc tgcttatcag aatactctcc acaaatgacg tttcagctta
    13081 cgatgttatc cagaaccttt tccctgacaa gtccaaggac ttcgttttca acatcactga
    13141 gagcgatatc gtgcttgtta aggaagtggc aaacggcgtt gagtcaaagg atctggaaaa
    13201 gctggcacgt tctatttctg ataccctttc aagtgaattc tatacaagag ttactgtagg
    13261 tataggaaca gtagtagagg gcgtaaagga tctggcaaga agcttcaagg aagcacagat
    13321 agctctcgag gtaggaaagg tgttcgatac tgacaagaat atcgtttctt atgacaatct
    13381 tggtatcgca agacttatct atcacctgcc tacaacactt tgcgaaacat tccttaagga
    13441 agttttcaag aaaggctcta tcgagtcgct ggatcatgaa acacttttca ctatccagaa
    13501 gttctttgaa aacaacctga acgtttctga aacttcaaga aaactgtttg ttcacagaaa
    13561 tactcttgtg tacagacttg aaaagatcaa aaagctcacg ggtcttgacc tgagagagtt
    13621 cgaccacgct atcatcttca agatagctct tatggtaaag aaatatctct ccgctaaccc
    13681 tgtcaagttc taagtcctac gagcgaaaaa gaggcttgct tgcctctgta aaactaaggc
    13741 ggtgtaaatt ttggtagagt ttaaagacgt gtccgttacc tattcaagcg gtgtggacgc
    13801 tctgaacaaa gtaaacctga agataaacga cggcgacttc gctttcgtgg taggtccttc
    13861 aggcgccgga aaatcaacac ttatcaagct ggttcttaaa gaaatagatg cgaccagcgg
    13921 tacagtaatg gtaaacggct tcaatctgaa aaagctgaga agaagaaaga tacctgcact
    13981 tcgcagaact atcggcgttg tttttcagga tttcagactt atccctacaa tgacagttta
    14041 tgagaacgtg gcgttcgtgc tgagagtaat cgacgctcct gcaaagtaca taagaagccg
    14101 tgttccgtat gttataagcc ttgtgggact ttccgataag gcgaaaagct atccgaccga
    14161 gctttcaggc ggtgagcagc agcgtgtggc acttgcaaga gcccttgtga acgacccaca
    14221 gctcatcatt gccgacgagc ctacaggaaa tatcgaccct gcgctttcat atgagatagt
    14281 agagcttctc aagggcataa acgagtgcgg cactaccatt cttatggtta cccacgagca
    14341 tgaccttgtg cgctatttcg gaggcagaat tatcaacatc aacaaaggtc agatcgcctt
    14401 tgacgaagtg ataggaggct ccaatgaaag caagtagttt tagatatctt atnnnnnnnn
    14461 nnatcagggc ttcaagggca tttggaaaaa cagaatgatg acgttcgcct ctttctgtat
    14521 cctgctcgtt tccctgctta tggtaggcac ttcggtgctg gtttccatta accttacaag
    14581 aataatcggc ggtatcgaag acaagagcga agtcatcgtt atcgtaaagg acggcacaag
    14641 tcaagctgat atgcagaccc ttgaaaataa gctcaaagag gtcggaaata tcaacactgt
    14701 gagcctttat tcaaaggacg aggcgtggaa aaatatgctc aagggcatga ctgatgagga
    14761 aaaggatttc ttccagtatg ctgacgataa cccattgcct gacacataca aggtaacggt
    14821 caaggatatc gagaagatga gcgaaacaac cactcagata gagacctttg acaatgttga
    14881 ctcaacaaag tcccctacag catttgctga tatccttatc agtatcagaa gagtatttac
    14941 gatagttgcc tttgcggtca ttgtggcact tgttgtggtt tgtctgataa tcatttcaaa
    15001 taccaccaga gcaagcgttt tcgcaagacg taaagagatc aacatcatga aatatgtagg
    15061 cgctaccaac agctttatca ggataccttt ctttatcgag ggtatgatag ttggtctgct
    15121 tgcagcagca ggcgcactgc tgctcacaaa gtttgcctac gagggaattt acaacatctt
    15181 caatgatgac ttcaagctct ggagcattct cggtgttcag aatttgtatt cgttcaaatc
    15241 cctcttgctc cctgttgcag catcttatct tatcgcaggc gctgttatcg gtgcggttgg
    15301 tacttctata agcacaggca agcatcttaa agtataatac atttgccaaa tatagacata
    15361 aggcaggcta tgatccaacg gggtttttag actgcctttt gttgcagttt aaagcatatc
    15421 ctgacatgag ataaaaaaga gagaacgtgc gggataatgc gttagaaaaa gataaaaacg
    15481 tgtaaaaatg gtctttttta agacggagga caggtatgaa aacaggtata ttcacaatga
    15541 agcgagtatt ggctttcact atcgccgtag tcgcttccat gacaatgctt gcaggcggca
    15601 cacagccggt catgacagca cagaacgctt ctgcagaaag tcaggcaata aagaacaaca
    15661 agaaaaaaat atcctctgcc aaggataaaa taagcgagct tgaacaaaag caggctgacc
    15721 ttgacaagca gataaacagc acaaaggacg atatctccaa ggaggaagaa aatcagaaag
    15781 ctatccaaga gcagatagaa actgttcagg aaactatact gacccttgag gattctatca
    15841 ctgaccttga aactgagata gccgaccttg aagaggctat cgcaaagagc gaaataaaaa
    15901 taaaaaacaa gagaactgag atagaaaatg gcgttgttga cttcaaacaa cgtcttagag
    15961 caatgtatgt tgccggaaat agttcataca ccgatattct tattggttcg acggactttt
    16021 atgatatgct tatgaaaata gagcttgtca aaagagtggc tgaccatgac aacaccatga
    16081 tcgacggact tgttgagctt aaaggcgagt atgaatctca ggaagcagag cttgaagcca
    16141 acaagactga acttgaaact aacaaaacta cccttgaaga acaaaaggcc tatcacactg
    16201 agcagaagaa aaagcttgac gacctttatg caaaaagtca ggctgttatc gaccagcttg
    16261 aggaagataa agcggcttat gaagccaata aggagcagat aaacaaggag caggaagact
    16321 tcgagacaca gctccagaag ctttacaagg agcaggagca gatcaaaaag aaggaagaag
    16381 aggaacgtaa gaaaaaagaa gaggaagaac gtaagagaag agaggaagaa gaacgtagac
    16441 aacagcagca acagcagaat aataacggca attcttcatc ttctaatgca aacggcaaca
    16501 cctctaacag cggtcaatct tctaccaata acggtgacta caattatacc gacaagagcc
    16561 agtttacatg gcctgtgccg ggttattatc atatttccta cggcgtgggt tggcgctggg
    16621 gcgcttatca caagggtatc gacatctggt ctcctggaat aagaggcgca aagatatgcg
    16681 ctgccgctga gggtactgtt atactcgtca gcaacacctg cacacacgat tatggcaaga
    16741 acggctcatg cggctgcggc ggcggatatg gaaactactg cataatcgac cacggcaacg
    16801 gctactggac cctttacggt cactcacagt atatgagcgt aacacagggt cagcacgtca
    16861 acaagggcga cgttctgggt attgtcggct caacaggata ttcaactggc gaccacctgc
    16921 actttgaggt gcgattgaac ggcgtggcac agaatccgtc aaattatgta taaacaggca
    16981 atgccgaaag gtcttgcccc acaaccaaag gagtctttta tttcatgaac aggctttaca
    17041 gcttaaaacg tattctcact ttcattatcg cattcgctgt taccgctggt attgcaggaa
    17101 gcagctacaa ttcttctttg aagataacct cagcggacac tgaaagctcg cagggggagg
    17161 actataacta tactgattct gtggctgacg gaggtcagca aagcgatgaa caaacagaaa
    17221 atgacgataa ttctcaggag gagtcaacag gttttgacat cacacagtat gcagaacagc
    17281 ttagagagct atcaaaaaag cagacggaaa taaacgaaca gctcgacaaa tatgatgagg
    17341 acattgagaa aagcgagaaa aagcagaagc ttctgaaaga aaagatagat tctataaatg
    17401 acgaaataga cgttctcaac tcttacatga ccgcactgga gcttcagata aacaccaatc
    17461 agcgaaatat cgataagaag caggctgaga taaacaaggg catagaggac tttaaacaaa
    17521 gattaagggc aatgtatatc gcagggtcgg acagctacac cacaatgctt ttggaatcag
    17581 acagctttta tgacatactc atgagattag agcttataaa gcgtgtggca caccatgatg
    17641 acactatcat tgataacctt ataaaactta aaactgaata tgaggatatg caaaagaccc
    17701 ttgacaaaca gaaagccgag tatgacgctc agtacacaca gtatacacag cagaaggata
    17761 tgctcagtca gctttaccgc tccagtacaa aaacacagca ggagcttaaa gctaagcaga
    17821 aaaagctcag agagcagaac gaagcctatg aggcggagcg tgagcagttt gagggcagcc
    17881 tttccggcat actgaaatcg tcaggctcag gcggaacagc aagagataac gaagtttccg
    17941 ccacaatggc acttgcagat gtgaagcttg aggagcttca caaggagata aatgaccgcc
    18001 ttgacaaggg cgaaaagctt ggagataccg agcctgatta ctacttcgca tggcctgttc
    18061 cgggggctta ctccgtatca tcaggcgtgg gcgcacgctg gggctcatat cacacgggtc
    18121 ttgacattcc tgcggctcac ggcacgccta ttcacgcttc atgcagtggc aaggtcataa
    18181 agattaacac cacctgcact catgactacg gcaaggagga gtcctgcggc tgtggcggcg
    18241 gttatggaaa ctatgttatc atcgaccacg gcaacgaatt cattaccctt tacggacacc
    18301 tttccgaggt agatgtagag ataggcgacg aggtgaaaaa aggcgacgtt atcggcaaaa
    18361 tgggttcgac cggcttttca acgggcgacc acctgcacat tgagatacgc tatcagggtt
    18421 atatattgaa ccctgcgttc tatcttgatg ttacacaata aaaaaatcga cctcttatgg
    18481 aaaaacataa gaggcctttt tttatcttat tttgaagcac tcacacgctt acaaagacgt
    18541 gaaataatcg ctgtgataaa tcttgttcct gcagtgcaaa gaagcattat cacggggata
    18601 aatgtgacga tatatctagg tggattttcc cagataagga aaaagagtat aactcccgtg
    18661 agagtcacta tgctgaaaaa caaaacagag tggcgatgct tgccagcggc ataaactgca
    18721 ccaaacgcca ttgctatgag cattgcactg tgatagccgt ctacatagct accaagtaag
    18781 cgactgttca atacctctcg caaaccacct gaggggtcgg agcgtgatag atatttgcca
    18841 taggaaaaac caccgctgtc ccacgttctg gcgactttgt gataagccat tttgcaggct
    18901 tcataagggg tgcttatgtt ttcaagacgg gcttttattt ctcggatatt cgcctgcttt
    18961 ttgtcgtcat atgtatcaaa tgacattgtg aagtcaacgt cctcgtcaac gtgggtcttg
    19021 tattcgctgt taaggctcat cattatccag tgtgtaacag ggaaacgata tctgtctacc
    19081 attttttcat cagagatacc catggcaaga ccgccatagt attccgcctt tgacacgcca
    19141 ataaacagta taacggccgc aaggctcagg atagctcccc tttttacacc cattttcagc
    19201 acaaaaaata tcattactga aatcagcatt actgcaatgc ttccctttac agtgtttcca
    19261 agtgcgataa aaaggcttgc tataagagca tagacaacgc ttttggtttt gcttttttct
    19321 ttcaccgcaa gggtcagaaa ataaagagta cagcttacaa atatcatgcc tgccgtgtca
    19381 gaatgaaaaa acggcgtata cagaagcata acaggcataa gtgctgaaat aatgccgcaa
    19441 aaaagccttt ggctgttaag tttgaatatc ctctcccctg tgagaaaggt cattattacg
    19501 ctaagttgaa taagaattac gttaaaggtc tgcccagcat agatgctcac gccgccggtg
    19561 aaaagcttcc ataaggcgaa aaatggtatc tgcaaatata tcatgcccca tgcgttaggg
    19621 tattttgcag cataaatacg gtagctttct ttgatgctcc ccagatcttc acccatgaca
    19681 aagcgttttg cgaactcccc tgttattttg gggtcaacca tttcgtaatt gtagtaaaga
    19741 taacgcacaa acaatatctg caatgcaagc aaaatcaggc agaaaaaagc aaatataagt
    19801 ctgacacgct ttttggtaag cttcttttca cagcgtttca gagcaatata caacactatc
    19861 gccgccgcaa ggaaaaatgg tccaagacaa agcattcgta tgttactgcg ttttatgtat
    19921 gagataaaca cagttgcagt gcaacagcag cacacgatca tgcttgtgac cgcaaaaaat
    19981 cggccggttt tcttcacaaa catttttatc tccttattag gagggatgct cctgagatat
    20041 ttttttacat gagttcgccc tgttttttta ttttgttcta tatttctaca tttgaattat
    20101 atcatcaacg gaaataagtt tcaattattt taacaactga aattgaatga actttttaaa
    20161 gtttttttag tgaagtaaac aaaaaaatcg ccgctctgtt gcaaaaaaca aagcagcgag
    20221 tattagtata agactattat tttgtgccga agatcctgtc acctgcgtcg ccaagaccag
    20281 gaacgatata agcgtcctcg ttgagctttt catcaaggtt gcctacataa atgtcgatat
    20341 cagggtgagc cgttctcaga gcgtcaagac cctcaggcgc agcgattatg cacatgaact
    20401 tgatgttctt gcagcccttg tctttcaaaa agtcaacagc ctgtatcgca gatccgcctg
    20461 tcgcaagcat tgggtcgcag acaataacaa gtcgctcgtt tattctatca ggaagcttgc
    20521 agtaatactc gtgaggctcg tgtgtgacct cgtcacggta aagaccgata tgaccgacct
    20581 ttgcagacgg aacgagtgca agaagtccgc ttaccatgcc aagacctgcc ctcaggatag
    20641 gcacgatagc aagctttttg ccgcagagca tagggagctt tcttgtggtg ataggtgttt
    20701 tcacctcaac gtcctctgtt ggaagatctc tcatagcctc atagcccatg agcattgcta
    20761 cctcttccac aagtatacgg aactctcttg ttccggtctt ttcatcacgc ataagcgtta
    20821 ttttgtgctt tacaagcggg tgattgatga tatttacgtt tcccattttc tgatttcctt
    20881 tcgtaatgat ttataaattc gcattttttt gtgtgcagta acgtgcgaac actgttcgcc
    20941 tctgcaattt tgttataatt atttcttggc tgcattgcct tttttctttg agagcataac
    21001 gtttgtaaga atgccaagga taagtgctgc cgctacttct gtgagtgtta tatcaccgaa
    21061 tgtgagtgtc agaccgccga tacctgcaat gaggataacg gacacaacaa agagatttct
    21121 gttatcgtca aggtttgcgt cctgtatcat tttaagacct gaaactgcga taaagccgta
    21181 aagagcgata caaacgccac ccattacgca ggcagggata gaatcaacga acgcaacaag
    21241 cggagtgata aacgaaaaca gcatacagcc tattgctgcg gcaaagatag ttacaacaga
    21301 agcatttccc gtgatagcaa cgcaggcaac ggactcgccg tatgttgtgt tcggacagcc
    21361 gccgaacagt gcgcctgcga tagaacctac accgtcgccg agaagcgttc ttgtaagacc
    21421 aggctcttca aggaggtcat ggtcgataat agaagaaagg tttttatggt cagcaacgtg
    21481 ttctgcaaaa acaacaagtg caaccggcac ataggctaca aatatagtag caaaatatgt
    21541 gaagtcgaac tccttaagac ctttcagacc tgaaacaaat gcaaagtccg gcaggctaac
    21601 aaatgttttc agtgaaacgc tgccatctgt gagcatatag ctgctgaaaa cgctgaaatc
    21661 tactatttta agtgcgtcaa tatcacatat attgccgata acagtgaaga tcgctgcggc
    21721 tgcatagcct gcgaggatac cgattataaa aggtataagt ctgagtgttt tcttgccata
    21781 tgttgagcaa agcattacaa ctgcaagggt aataagtcca caaacaaaag ccacatatga
    21841 cgagcctaca ggcacgttgg atacagtttc gattatctca ggtgtgccgc tgtcggtggc
    21901 gactgcatac tctgttacct gctttgtgac gtcgcctttg acgaggtcgc cgatagcgtt
    21961 tcctgcaagg gaaagtccga tgatagcaac ggtcggtcct attaccaccg caggcatgag
    22021 cttattgagc cagccaacgc caacaaattt tactactatt gcgataacga catatacaag
    22081 tcctgcaagg aatgcaccga cgataagtcc aagcatgcca agctgcattg aaacgccgcc
    22141 tgcaaaggcc gccgccattg agcctaaaaa agcaaaggac gagccaagga acacggggct
    22201 ttttcttttt gtgaaaaaga tatagactat tgttcccaca cctgcaccaa acattgcagc
    22261 cgcaggagta agaccgttgc cgataataag cggcacagcg atggtcgccg caaggatagc
    22321 cagcatctgc tgaagtgcga acactaccag ttgaccagcc tttggcttgt ctgaaacatc
    22381 aaaaataagt ttcaaaataa cacctcattc atattttttc ttttcccata taaatggaaa
    22441 acacttcttt gcgatacgtt ttgactcaaa ccgccttttt aattttaact tataacaata
    22501 tttttgtcaa tgagctaaat gccgtatttc agaaggattt tttgtgcgta attgcccatt
    22561 ttctttgaca aataagctgt tttgcgttat aatagcaatt gatatattgt attatgtgag
    22621 gtaatgacat gaaaaattac agatatgttc tttttgacct tgacggcacg cttactgagt
    22681 cgggcgaggg cataataaat tcggctgtat acgcactgaa aaagttcggc atagagcctg
    22741 agatgagcac gatgacaaag tttctcggcc cgccgctgaa agactctttc agggattttt
    22801 acggctttga tgatgaaaaa tgcgagcagg ctgttaaata ctaccgtgaa tattacacca
    22861 ccaagggcat tttcgagaac cgtgtttatg acggcgttga agaatgtctt aaaaagctgt
    22921 cagaggctgg gcttcacctt gcggtggcga cttctaagcc gcaggtcttt gcggtgaaga
    22981 tactggagca ttttcacctt gcacagtatt ttgactacat aggcggtgcg gagcttgacg
    23041 gctctaggag cgtaaaggcg gacgttatcg tgcacacttt gaaaaactgc ggagttaatg
    23101 accgctcaca ggtgataatg gcaggcgaca ggtatcatga cgtactgggt gcaaaggctc
    23161 ttggcataga ctgcatcggt atgctttacg gctacggcac aagagaggag ctttctcagg
    23221 caggtgcggt ttatatagcc gaaacgcctc tggaagccgc aaaatggctt ctgggataaa
    23281 tttgacaggt gtacaaaaga gcgaaaacct attgcaaaag tcaaatgttt atgatataat
    23341 aaaataaatt attatgtggc ggcataagaa ataagcttgt gatcctgcca cgatcaagag
    23401 gagttgtttt ctgtgataaa gtttcgcaac cacattggaa ctataggtat atccactgga
    23461 tatctcagac agcttatctc ggatactgcc gaaagctgtt tcggcgtggc aggacttaac
    23521 gcctatggtg caaaacaggg cgtttctgca atgctaaaga aagagaacac cacaaagggc
    23581 gttataatcc gtcaggacgg ggacgagctt atcgttgacc tgcacattac tgtgaactac
    23641 ggcataaatg tcggagctat cgttgacagc atcattcaca aggtaaatta tgttctgaca
    23701 gagcaggcag gtgtcaacgt taaatctgtg aacgtttacg ttgacgaaat gaaaggctga
    23761 acgtcctagg atatctataa caatatagga ggaaaactat agtgataaac ggaggcttgc
    23821 tgcgtgacgc tgttataagt gccgcacaca gcatatctaa caagaaaaaa tcagttgacg
    23881 agcttaacgt gttcccagta cctgacggag atacaggaac aaatatgtca atgactataa
    23941 caaattccgt agcagagctt gaaaatctca gcgacgatac agactgctca gttgtggcaa
    24001 gcacaacagc ttctgcactt cttagaggtg caaggggaaa ctcaggcgtt atactttcgc
    24061 ttattttcag aggctttgca aaaggacttg caggcaaaaa ggaaatgggt gcgaatgagc
    24121 ttgtggagtc gctggaacag ggcgtttcag gagcatacaa gagcgtaatg aagcctacag
    24181 agggaactat cctgacagtt gcaagagagt ctgcggagaa agcaagagaa atgctccact
    24241 ccacaaacga tcctattatt ataatggagg aagtttgcaa acaggctgaa ctttcactga
    24301 acgaaacacc aaagcttctc cctgcacttg ccaaggcagg cgttgttgac gcaggcggca
    24361 agggacttct cattatcttt gaagcaatga gagatgtatt caagggtggc gaaatagtaa
    24421 aggccgatgc tcctgcacag aaacagcagg taacagtcga cacatttgcg gctactgttg
    24481 gtcagtatga ctgtgagata cacttcacat actgcactga aatgataatc acaaagaaag
    24541 ctgacgctga cgattcatca aagctgagag cttatcttga aaccataggt gactgcgttg
    24601 tagttgtcga ggacgatgag ataataaagg tacacgttca taccaacgac cctggtaagg
    24661 ctatccagaa gggtcttgaa tttggctata tcaatctgcc aaagatagaa aatatgaagc
    24721 tccagcacaa ggccagacag aaagaagcaa aacaggagtt cgttcctgca aagcctgaaa
    24781 agcagtacgg ctttgttgca gttgcggcag gaaacggcat tgagtcaatg ttcagagata
    24841 tcggtgttga ctgcatcgtt aagggcggac agaccatgaa ctcgtccaca gaggatatac
    24901 ttgcggcaat aatgtcatgc cctgctgaaa cggttttcgt tctgccaaac aacaagaaca
    24961 ttatcatggc ggctgagcag gctgttaagc ttgcagacag aaaggtatgc gtgcttcaga
    25021 caaggactat accgcaggga atgtcagcaa tgctggcatt tgaccctgac gctgatttca
    25081 acacaaaccg cattgagatg accaatgcac ttgaaagagt tgccacaggt caggtaacat
    25141 ttgcggcaag ggattctgac tatgagggtc acgctatcaa gcagggcgag atactctgca
    25201 tcgaaaacgg caagctgagc tttgtggaaa aggacgtttc aaaggcagct tacaagctca
    25261 caaagaagct tgttaagggc gacagctcat tcatcacgat cctttacggc tcagacgtta
    25321 cagacgaggc ggctgaggca ctgcaaaagc aggtagctgc gaagttctca aatcttgatg
    25381 taaaccttat aaacggcggt cagcctgttt actactacat catttctgta gaataagata
    25441 acgaggcgga cggcattgag ctgtccgctt ttttgtacaa tggagaaatt tttatgagtg
    25501 atataacaaa ggttcagtgc agaaaatgcc tgttggagga tatggacgaa aatgattttc
    25561 tacgtgatat gcgttcgcac attgcggcat atcctgcgga caagagggta tcggaggacg
    25621 aatatcatcg gcgtttgaca ttttgcaagg attgcgaaaa gcttgtggac ggaatgtgcg
    25681 ttttatgcgg ctgttatgtt gagctgaggg cgttgaagat aggtatgcgt tgtgcggata
    25741 tagacaagaa gtggtaaagg catactgttc attcaggcaa gttccttccg ccgcaaaatc
    25801 gctgtcgctc tttgcgggtt ttactttgtt taatgggata ggaaaaagcg gtgcagagat
    25861 tttttctccg acaccgcttt attatttctt ttactgttgt gtgctttttt acttttctgc
    25921 accgcttagt cggctgtcgc cgaatgaggc tctgcctcat actccgcagg ggctttgccc
    25981 cttgacccca caagccctct agcgccgggc ttgagcctgc gttttatctc tcgctacgct
    26041 cggtgttggg ggcacaactt tggtctgcta tgctcattcc tcattcctca ctcctcactt
    26101 atgacattgc ctcttccatt tcctttacaa gctctccgaa cattgcaagt gcctcattga
    26161 taggctgagt tgttgccata tctacacctg cacccttaag taatgagata gggtcagttg
    26221 agcagccgcc tttcaggaag cctatatagt cctttaccgc aggctcacca tacttcaata
    26281 tcctctgtga aagtgcaata gccgcagaat agcctgttgc atactgataa acataatagt
    26341 cataatagaa gtgaggtatc ctagcccact ccaattttat ctcatcatca agcactattc
    26401 cgtcaccgaa ataaagcttg tttagctcgc cgtaaagctc gttcagcccc tcggctgtca
    26461 gactctcccc tgccgctacc atttcattta ttttcagctc aaactctgcg aacattgtct
    26521 gacggtaaag tgtggtacgg aactgttcaa ggaagtaatt tataagatac gctctctgct
    26581 ttttatcctc ggtgtttttc agcagatact gcataagaag ggcttcgttg caagttgagg
    26641 ctacctctgc cacaaagata acgtaatctg aatataccac aggctgattt ttatttgaaa
    26701 gatacgagtg gatagcgtga cccatttcgt gcgccagcgt gaacatacag ttaagtgtat
    26761 ccttatgatt gagcagtaca taagggtgaa ctcttgcacc tgcggaatat gctccgcttg
    26821 ttttgccctc gttctcgtaa acgtctatcc agcggttttc aaagccctct ttgagtatcg
    26881 caaggtaatc ctcgcccata ggtgcaaggg ctttcaggac agtttcctta gcctcctcaa
    26941 atgttatctt catatctgcg tcaggaacaa caggagtata aaggtcatag aaatgcagct
    27001 catcaacgcc caaaagcttc tttctcagtg caacataatc gtacatatag tgcatattgt
    27061 catggacggc ttcaataaga tttttgtaca ctgaaacagg cacttctgtt ccgtccaaag
    27121 cggcttcaag gcttgaatca tatcttcttg cctttgcata gaagttcacc gcttttatct
    27181 gtgccgacag tgttgccgca caggtgttct tgaacttgtc aaatgtgccg tacatagact
    27241 cgaaagccga ctttctcagc actctgtcct cgctctgcac cagtggtata taacttccgt
    27301 gtgtgacctg atgagccttg ccgtccttat ctacagcgtc agggaaacgc aggtctgcgt
    27361 cagaaaacat tgagtagata ttctcagggg actgacccat ttcaccagtc agagcaagta
    27421 tcttctcctc agcctcagag aggatatgag cctttttacg acgtagctta tccagcgcac
    27481 gcttataaag cttcaactca ggcttatcct catagaattt ctgaagctta tcctcctcga
    27541 tagaaatgat ctcaggtgtt tcaaagcttc ctgctgagtt tactgcaaca taagcgttca
    27601 taagctgacc gagaaagccc tgatactttg catttgcagt gtcctcgtca gaacgtcttt
    27661 gagcatagtt gccaaggctg tcgcaaagaa cagatatctc gtctgaaagc tgcataaaat
    27721 cataaagcgt gtcagcactc tcgctaagcc tgcccttgaa tgccgctatc ctctcaggca
    27781 tagccttaag cttctgcaag tcctccgccc acttttcgtc agtggcataa atatcctcca
    27841 gtgcccattt gaactgttca ggcacttcac ttctcttagg tattctttcc tcgctcattt
    27901 tcatgacctc gtttctataa taataggtat aggcttgtct tg
//
LOCUS       DAWEMR010000010        25377 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_787_length_25377_cov_9.635613, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000010 DAWEMR010000000
VERSION     DAWEMR010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 25377)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25377)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25377
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_787_length_25377_cov_9.635613"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3900..3909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6444..6543
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8207..8216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19755..19764
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6815 a         5706 c         4835 g         7891 t
ORIGIN      
        1 ccaccttatc cttatccttt acaacgctgc ccacctcgcc gccgatatac cttgacggac
       61 gctgcacttt aagcagcagc ttttctattt tctttttcag ttcttccaaa gttttatcct
      121 ccgatattga atgtctgtac tatctcaccg cacccggtaa agcgaaagca atacacaaag
      181 tgaggtgaaa cgggcgaaca ctgttcgccc ctacagatac ataagcattt catattatta
      241 taccaaaaat ttattacaaa agcaataagc aaagtgttaa tatatgactt ccgagaggac
      301 gataaaacag aaagttgcgg caaaagaaac gctcacagtg ccgtggagta tcatatgtat
      361 catagccacc gctgagagta gtataaacac cgctctgaaa aactcgcaga ccttgccatt
      421 ggcaagcaag gcgagtatcc tgccaccgtc aaaatagcga aatggcagta ggtttagcac
      481 accaagcaga aaattcacct gacagaaaaa gccaccacca tttgttagat atgttagcag
      541 gcagagggca aaatttactg ctgcaccacc gaaaagcaca acaagatcgt gatttttaga
      601 cagctgttta ttatcaggag taatgcggat accgcctcca tagacaacaa tttcttgcgg
      661 tgagcttccg aaagcggtca tgagtacgag gtgacccagt tcgtggagtg cgcagcacat
      721 aagcgtaaca aagagcatat ccgcctgcgt acctgagcag acagacacaa gtgcgacgac
      781 gaaaaagaaa ctgaacgaca cgccgtaggt ggtatttttt atctttattt taaacaagtt
      841 catcaccgag atatttatat tcggtatttg ctaaaataat gtgtaggggc gaacactgtt
      901 cgcccgtcga agaaataaac cctgattatt acgctgtttg gcaaacgtaa aaaactcccc
      961 tacagttctg aatttgtaca agttctgagc acgggcgaac gctgttcgcc cctacagatt
     1021 attcgggaaa ggttttactg ccgcaaaatc gctacgctct ttgctggtgc tttgtttgag
     1081 ttatagaaaa agcggtgtga gttttatttc tcgacaccgc tttgattatt ttttgtttgg
     1141 tagtttcatt tctgcacccc tctgcactac atagcagaat aaacaaagtg catacattta
     1201 cctcgtactg aagagccaca agggtcagct atgccaattc ctcactcctc acttaaactt
     1261 gcctacagca tcttcttcaa atactgtccc gtataagacc tgtcgcaagc cgcaatttcc
     1321 tcaggcgtgc ctgtgcaaac gatagttcca ccgccgtcgc caccctcagg tcctaagtct
     1381 atgatatggt ctgcggtctt tattacatca agattatgct cgataacaag cactgtattt
     1441 cccccgtctg caagcttctg caatacctct ataagcttgt gtacgtccgc agtatgaagt
     1501 cctgtagtcg gctcgtctag gatatatata gttctgcctg tggctctttt tgaaagctct
     1561 gttgcaagct tgactctctg cgcttcaccg cctgaaaggg ttgtggccgc ctgccctatc
     1621 ttcacatagc caagaccaac ttcatacagt gtttcaagct tacggcttat tttagggata
     1681 gcactgaaaa actcaacgcc ctcttccact gtcatttcaa gcacgtcata gatgttcttg
     1741 cccttatatc taacttccag cgtttcacgg ttgtatctca taccaccgca gacttcacat
     1801 ggcacataca cgtcaggcaa aaagtgcatt tctatcttga ttattccgtc gccctcgcag
     1861 gcttcacatc ttccgccttt gacgttgaat gagaaacgtc ctgcaccgta gcctttcatc
     1921 tttgcatcag cggtctgtgc gaaaagttct ctgatatccg tgaatacccc tgtataggtg
     1981 gcaggattag accttggtgt tctgcctatt ggcgactggt caatatctat taccttatca
     2041 agatgctcta tcccctgcat ttccttgaat gctccagagc gtattttcgc gcgatttaat
     2101 ctgcctgcaa gttctttata gattatctcg ttcacaagcg atgatttgcc tgagcctgac
     2161 acacctgtta cgcaggtgaa agtgccgaga ggtatctcaa catctatgtt tttcagattg
     2221 ttctgtgttg caccgatgac tttcaggaac ttgccgttgc cctgtctgcg tgtttcaggg
     2281 acaggtattt tctttgcacc gctgagatac tgtcctgtaa tagaacgctt gcacttcatt
     2341 atatcgtcaa cagttcctgc acagactatc tcgccgccgt gaatgcctgc ccctggtcca
     2401 acgtcaacca cgaaatctgc cgcacgcatt gtgtcctcgt catgctcgac cactataaga
     2461 gtgttgccta ggtcacgaag cctttttaag gttgcgataa gcttgtcatt gtcacgctga
     2521 tgcagaccga tagacggctc gtctaggata tacagcacgc ccacaagtga tgagcctatc
     2581 tgcgttgcaa gccttatacg ctggctctca ccgcctgaga gagtgcctgc ggaacgtgac
     2641 agggtgagat attcaagtcc cacgcttttg agaaagccaa gacgtgactt tatctctttt
     2701 attatctgct caccgataag cttgtcacgg tcgcttatct caaggttttc aaaaaactcc
     2761 agtgcctttg ccaccgactt gtgacaaagc tctgcgatat ttattccacc aactgtaacg
     2821 ctcaggctct cttttttaag cctttcacca tggcaggcag gacacttttc atcgctcata
     2881 taagcttcta tatcagcctt tgcccaatcg ctgttggatt ccttgtaacg cctttcaagg
     2941 tttggtataa cgccctcaaa ttcagcctga tagtcagcct tatagaactt attgccacgc
     3001 tttaggtgaa gcttctgacc ttgagttcca taaaggaaaa tgtctatagc atccttgtcg
     3061 aggtttttca caggctcgtc aagggatatg ccgtaggtct cggaaatggc gttgaaatac
     3121 atcatggcga tagagccttc ttcaaggctg ttccagccgc tggctttgat tgctccctgc
     3181 cttatggaaa ggtccttgtt agggataata agctcagggt ctatcttctt gaacacgcca
     3241 agacctgtac acttttcgca agcaccgaaa ggattgttga atgagaacat tctcggtgtc
     3301 agctcgtcga tagaaacacc atgctcaggg caggcataat tctgtgaaaa aagcatttcc
     3361 tctccgccga taacgtcaac ccctacaagt ccccctgtaa gcgaagaaac agtttcgata
     3421 ctgtcggtca gtcgggactt tatgccctct ttgataacaa gtcggtcaac gactatctcg
     3481 atagtgtgct ttttattttt ttctattttt atctcttccg aaaggtcata ggttatgccg
     3541 tcgatacgca ctctcacata gcccgacttt cttgcacggt caagctcttt tgtatgctcg
     3601 cccttacgac ctctgatgat aggagctaaa agctgaatct ttgtaccctc gtcaagctcc
     3661 attatcttat ccactatctg atccactgtc tgctgacgta tctctcttcc gcagacagga
     3721 cagtgcggca cgcctatacg ggcgtaaagc agtctgagat agtcgtatat ctcggtgact
     3781 gttccaacag ttgaacgtgg gtttttggaa gtagtttttt gatctatgga tatagcaggg
     3841 gaaagcccct ctatgctgtc aacatcaggc ttgtccattt gacccaaaaa cattctcgcn
     3901 nnnnnnnnng cataagatga cagcgactca acataacgtc tttgtccgtc agcgtatatg
     3961 gtatcgaatg caagagaaga tttacccgaa cccgaaagac ctgtcataac aacaagctta
     4021 tctctcggta tggtaacatc aacatttttg agattatgct ctcttgcacc ttttataact
     4081 atctcgttaa ttggcatttg tattctttac tctccttttt atatacaaaa aatcacatat
     4141 tagtgttcgc ccctacacac gttttgtggg tgtatatccg ttttatgttt cgcaaggctt
     4201 tttacttctt ctcacccttt aactccgcaa ttttatcacg caagaatgcg gcgtgctcaa
     4261 attcaagcag ctttgccgcc tctttcattt cctttgtgag cttttctatg agctttgcag
     4321 tttcctgctt tgaaagcttt gttttctgcc ttgcctcgta ttccacctct tccttggtgg
     4381 atatttctat tacatctctt atatccttga cgatagtttt cggaactatg ccgtgttctt
     4441 cgttgaactt catctgtatt gcacgacggc gctctgtttc ggtgatagcc ttttccattg
     4501 aacgtgtcac ggtgtcggca tacattatta ccttgccctc agcgtttcgt gcggctctgc
     4561 ctatggtctg tataagtgcg ctttcagaac gcaggaagcc ctctttatcg gcgtcaagaa
     4621 tcgccacaag agttacctca ggcaggtcaa gaccctcacg caaaaggttg ataccaacga
     4681 gcacgtcaaa ctcgcccaga cgcaggtcac ggattatttc catacgctcg atagtgtcga
     4741 tatcgtggtg catatatctc acctttacgt caaagctctt gagatagttt gttagatcct
     4801 cagccatttt ctttgtaaga gtagtaacaa gcacacgctg atttttagct gtgcacatat
     4861 ttatctcgct gagcaagtcc tctatctgac cctcagtcgg ctttacgatt atctcagggt
     4921 caagcagacc tgtcggtctt atgacctgct ccactatctg ctgtgacctt tcctgctcga
     4981 taggtcctgg ggtagctgac acgaaaaccg cctgattaat atgagaataa aactcatcaa
     5041 aagtcagcgg tctgttatcc attgctgacg gtaggcggaa gccatagtca acaagggtct
     5101 gttttctcgc tctgtcgcct gcatacatac ctcttacctg cggcagagaa acgtgtgact
     5161 cgtccacgaa aagcacaaaa tcctcaggca gaaagtcaag gagagtgaac ggcgtagagc
     5221 cggcaggacg tcttgcaagc acacgggaat agttctcgat accgctgcaa aagcctatct
     5281 cctccaacat ctcaatatca tagttggtgc gctgtgcgat acgctgagct tcgataagca
     5341 tatcgttgtc tttgaaatac ttgatacgct cgtcaagctc ctgcttaatc tcttcgatag
     5401 cgtcgtgcat tttatcacgg ctgacaatat aatgggaagc agggaaaatt gcagcatggg
     5461 caagggagca aagcacctca ccggtcacca cgtttatttc agtgatacgg tctatctcat
     5521 ctccgaagaa ctccactctt atggcagtgt ctgtagaatt tgcagggaat atctccacca
     5581 catctcccct gaccctgaac ttgtttctca cgaaatttat atcgttacgc tcatactgga
     5641 tacccacaag gtcagcgata agtctgtcac gggatttctc cataccacgg cggatagaca
     5701 ccaccatgga cttatactcc tcagggtcac caagggagta gatacaagaa acgctggcaa
     5761 cgataataac gtcacggcgt tcagcgatag cggaggtagc ggaatgtcta agcttatcta
     5821 tctcgtcatt aacagagctg tccttttcga tatagacgtc cttgctgggc acataggctt
     5881 caggctgata atagtcatag taggacacga aatattccac ggcattgttc ggaaaaaact
     5941 ccttgaactc agagcaaagc tgagcggcaa gtatcttatt atgggcaagc acgagcgttg
     6001 gacggtttat cctagcaata acgtttgcca tagtaaaggt cttgccggaa ccagtaacgc
     6061 ccataagggt ctgctctctc atgccgcttt cgataccctt taccagagcg tccaccgcct
     6121 caggctggtc gcccgatagc ttataatctg aaacaagctg aaacttatcc ataaatgcct
     6181 cctcaatcga acagttgttt taatcattat attacattta tttttaattg taaaggcttt
     6241 tttatcaata ttttttgaat ttgcgtatat gtacgatttt tcggactggt attgttactc
     6301 aaccttgcgg tatcagctcg atgtatgtgt aggggcgaac accgttcgcc cgtcgaagaa
     6361 acaaacctac ggtaacaaca aggttcggca gcaaaaaatt ctccccgaca attctgaatt
     6421 tacagcaaat cctgagcacg ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnncagcaaa aaattctccc cgacaattct gaatttacag caaatcctga gcacgggcta
     6601 cagattattc gggagagttt gtcatgccgc aaaatcgcta cgctcggtgc agggggcaca
     6661 actttggtca gctttgctca ttcctcactc cacactcctc actcctcact taataccgcc
     6721 ccctcttgca ttatcccaca ctttaggtta taatatcact atagtaataa tttcaggagg
     6781 acgtaacatg aatagatcat atgacgaata caactacacc ctctcaaagc gtgaactgga
     6841 agagcttaaa ttcttattca tctctgatga tgtggactac aacgacactg acagcaatgg
     6901 gcttactttc ctgctcactg caatcgaaag tgctgacttt gttgagcagg acggcgttct
     6961 caagcctgac aagcgaagtg ttgatttcat caaatggctc atcgctcaag gctcagatgt
     7021 aaacttccct gctgacaaaa agcctttaca atatgctatc aaatgtgaag aggagatatc
     7081 tcgccgtaaa ggcaaagcct gcgatatgtc agaaattctt ctgcttctcc gtgaaagcgg
     7141 tgctgacagc accggtcttg acgagcttat tgacggagga ttctccgctc gtgtgaagct
     7201 ctgaggtatg gcatgagaga atttataata aatcagaatg acgcaggtca gcgtgttgac
     7261 aagtttctca caaaggctgt gccgaagctg cctaaaaatc tgctgtacaa atatctgcgc
     7321 ctaaagcgta tcaaggtcaa cggcaaacgc tgtgagatat ccacacggct tgcttgcggt
     7381 gacgttatgc agctttacat caatgacgaa tttttcgagg gcgaagttgc tgacagacct
     7441 gcttttctca gtgcgccaac gtctttgaat attatttacg aggacgagaa tattatcctt
     7501 tgcgacaaaa agtgcggact tgtggtacat gaggacgaaa gcggttcggc ggacacactt
     7561 ataaacagga tacagcacta tttatatgaa aagggtgaat atcgtcctga ggaggagctg
     7621 tcttttgctc ctgccctttg caacaggatc gaccgcaaca caggcggcat tgtaatatgt
     7681 gcgaaaaatg ctgaaagttt gcgtgtgctt aatcagaaag ttaaagacag agaacttgaa
     7741 aagctttatc tttgtgtgac agtgggcata ccaaaggaaa agtctgctcg gctcacggct
     7801 tatcatcaaa aggacgaggc gacaaaaact gtcaaggtat ctgacaaaaa gtttcagggc
     7861 agccgcacca tgataacctc ttatcgtgtg cttgcggaaa acaaggctga tgaccttgca
     7921 cttctcgaag ttgacctcat cacaggcagg acgcatcaga taagggcgca tcttgctcac
     7981 attggctatc cacttctcgg tgacggcaaa tacggaataa ataaagtcaa ccgtgcatac
     8041 aatgtgaaaa cgcaggcact ttattcgtac aagctcacct ttaaattcac cactgacgca
     8101 ggtatacttg aatatctaaa cggcaagagc tttcaggtta aagacgtatg gttttgcgaa
     8161 aaattctttg gatataagct ctgatatttc gttaaatcgc actattnnnn nnnnnntgtg
     8221 gatttgtcat atatgacaaa ataatataat tttcatgcaa tttttgtgta aattgttcaa
     8281 aagtgcttgc aaatgtatat aaaatatgtt ataattattt agtcaaattt aacggctgta
     8341 ctatatatac agaaaatgac agatatattt tatagatttg gaggaacaaa ggtatgacag
     8401 gagatctgca ttgtcacaca acactgtctg acggctcgct tggtatagaa gaggtcattg
     8461 ttcaggcaaa aagaatggga cttgactttc ttgcgataac agaccatgac actctttcat
     8521 catcaaacag agcgcaaata cttggcgaac gttatggggt caaggtcatt cctgcggtag
     8581 agctttccgc atgggacaag aagcgcaatt caaaagtaca cattctttgc tatgcaccgc
     8641 agaagccaga caggctggaa ggtctttgcc tgaagtcatg ccagataaga aaagactgtg
     8701 caaaggaaat gatagaaaag gtaatggcaa gatatcctat ccctgccgat gctgtactgc
     8761 actacacaaa aagctcaaag agcattttca agcagcatat catgagagca ttagtgaatt
     8821 acggctatgc cactgagctt tacggctctg tgaacgacaa gctttttgca tatccaaacg
     8881 gcgaatgtct tgttacccgt gaatatcctg acgtaaactt tgtgcttgat cttatccatt
     8941 ccgcaaaggg cattgctgtt atggcacacc ctgttatgtt caacaacacc gagctgctcc
     9001 ttgagcttat cgaggcaggc aagcttgacg gaattgaagc atatcattac tctgctacac
     9061 caactcaaca gcagaagctt gtggatatcg caaaggaaca tgacctgata gtgaccggtg
     9121 gctctgactt ccacggtctg tataatgaga ctatgacaca tatcgggagc atgaccactg
     9181 acaaggaaaa tcttgataag ctgttcaagc tcattcatat aaattcacaa aaagctaaca
     9241 aagcggctgt gaaaacagag aaataatatg gtataataaa gggacattat catgcggtaa
     9301 tgttccttta atgcgtaaaa tagagaggac tgtataaaat gaacgtaatg gaagaatccc
     9361 tacagaaaca cagagaatgg aaaggcaaga tagaggtctg ctcaagagca aaggtaaaca
     9421 atgcaaaaga cctgacgctg gcttacacac caggcgttgc tcagccttgc cttgagatac
     9481 agaaagatcc tgacctttca tatgagctta caagacgtgc aaatcttgtg gctgttatca
     9541 cagacggctc tgctgtgctt ggtcttggca atattggcgg tctggctggt atgcctgtta
     9601 tggagggaaa atgcgcactt ttcaaggagt tcgcagacgt tgacgctttt ccactttgca
     9661 taaagtccaa tgacgttgac gaaatagtaa acactatcgc tatgatatca gacagcttcg
     9721 gcggaataaa tcttgaagat attgccgctc caaggtgctt tgagatagag gctaagctta
     9781 aagaaaaagt tgatatccct gttttccatg acgatcagca cggaactgct atcgttgttg
     9841 gtgcggcact tatgaatgcc tgcaaggtgg ctaacaagaa gatagaggat attacacttg
     9901 ttataaacgg tgcaggtgca gctggctgtg ctatcggaaa gctgctgctt tctctcggta
     9961 ttggcaatct tatcatggta gaccgtgagg gcataatctg cgagggcgac aattatctta
    10021 acgaggctca cgctgaaatg gctaaggtca caaacaagaa caagctccac ggctctcttg
    10081 ccgacgcact caaaggcgca gacgctttcg taggtgtttc caagccaaag cttgtgaccg
    10141 ctgaaatggt cagatccatg aatgataagc ctatactttt cgcaatggct aacccgacac
    10201 ctgagataat gcctgacgaa gcaaaggcgg ctggtgcgtt tatcgttggc acaggacgtt
    10261 cagacttccc taaccagata aacaacgtta tcgctttccc tggaattttc aagggtgcgc
    10321 ttgcagtaag ggcttctgac atcaacgagg aaatgaaaat ggctgccgca aaggctatcg
    10381 caggctgtgt gcctgaggac aagctcaatg cggaatttat actgcctaac tcttttgaca
    10441 ggtcagtacc ggtcgctgtg gctgaagctg tggcaaaggc tgcaagagaa acaggcgtag
    10501 caagaattta ggaggttttt tcaaatggca actataagct acactccaaa gggagtatgt
    10561 tcaagaaaaa tagatatcga agtagaggac ggtatcgtaa agaaagtgca tttcacaggt
    10621 ggctgtaacg gcaacacaca gggtgtatca aagcttgttg agggcatgaa ggttcaagac
    10681 gttattgaca gacttgaggg tatcgactgc aacggacgtg gcacatcatg tcctgctcag
    10741 cttgctgagg ctttgaagtc tgcactctaa gctttcgtac tgaacataac acggatataa
    10801 taacgggcga acactgttcg cccctacaaa agaaaacgga ctgcattttg attgcagtcc
    10861 gtttttttgt acatattcta attttctttc gttgcgcttt gagtgaaact cattgaaaca
    10921 aatcccgtac cagttaccgt aatatcggta tgattaccgt cagaccactg ttctgcactg
    10981 ttaatgccgt tcgttctata gaactgaata tcatattgat ttttttcagc agaattgact
    11041 tttaaagtgg cagtggctat gccagaactg tcctttgaaa taatcagatc ataagtgttt
    11101 ttaaaccatg tgctgatatc aacatatttc gtgttccatg tcagtgtgac agtaccctcg
    11161 ccgctgcctg acaggcgata gttgaagccg tcaagatctt taggcgaagt agcattgtcg
    11221 atgaatgtgc ctgtccatgt tgctgatgtg ttatcattta tatagataac gccaagcttt
    11281 gcggagagcg ttttcaactc tgcctgatct gtaggtgcgg ctgtcacttt catgtatata
    11341 tcagactttg tgagctgcga cttatcaaag gtcagcacaa attcatgctt gttgaaatca
    11401 tctgatttaa gcacaagtga agaaaataca atttttgaat taccttcaaa agtcttattt
    11461 tcaccgccat attcaaccga gtatttagca gcttcttctc cggtaacggc atttccgttg
    11521 ctgtccacaa gctcggctgt caaagtgtat gtgatatcct tgtcattatg ctttatcaat
    11581 gtttgcggat agttgcacac attgactgtc acacaaggcg agttttccat actgctaaaa
    11641 gttatcgtcc ttggaagatc attttcatct gttgtaagat aattagatga aaacagcatg
    11701 ccagctccct tttgtgtgga tacaactcgc tttacacttg aaacgctggt ataagccgcc
    11761 aagacaccaa tagtactgag tgcggctgcc gcaaaagcta tccatattgc aagccacgat
    11821 cttttaagct tttttataaa ttggtctttc ataacagcca ccctcctctc tattttgtga
    11881 tttattactt tctctgaact gtcaggtaga ccatgtcagt ttctttattg ttttggacgt
    11941 tttcattcca ttttactctg attataaaat aatcgcaaaa cggatcttta ttgtcaatta
    12001 ccgtcagtgt atccgattgc caataaagtg cttcggaatt tttctgaaca ttgctatagg
    12061 tgtcataaga cttttcgtga agtgtgctgt tagcaagtat ctccgagcct tgctgattga
    12121 gataatgtcc cgagatctta ttgccgtcta ttttgtaata aaacgtttta ttctcttcat
    12181 cagaaacata aacgacagct tctgattttt cagtttcact gacgcttttc gcacgatata
    12241 tctcatactc aaaagggata ttcgttgtat gtgcaagctg tatttgatat tgttgcacgt
    12301 ctgccccgcc tatgccgaaa acaaagtctt tataactgcc gttctcaacg tctatatctg
    12361 ccatttcaag atttgccaca gattctttgt ttcctgcggt gatataaagc tgagtagggg
    12421 catttatttt tgttgtaaga gcaatagaac gtttgtgagt aaaccacgca taaacaggca
    12481 gtgccacaag cagtaccacc gggatggata ctgctatgac tagctggagc ttcttctttt
    12541 tttctaattt tctgaatttt ccaaaaatgc tttttgtcat aattttctcc tgaaaatgcc
    12601 tgcttattat gaatttataa agcctgtttc tatttttgcg tttgtgtaaa caacatagtc
    12661 gcttgaggta gttccattaa caactgagtt gttgtcatta agcaatactg cggtaagctc
    12721 aactctatag ttcgcatagg taagtccctg ctcctcaaaa gccttgcctg tttttactgt
    12781 aaatttcgta acggctgtgt tttgttcttc gccattatag ccagtggtaa aaatgcactc
    12841 cttgccattc agaccgctgt ttgatgtggc gttttccaaa gtaaagctac tgaggtattt
    12901 gctaatatct tttgtctgct tatattcgcc actattgtcc ttaacataca acctcattgt
    12961 atactgtatt tttttgccac tgtcctttgt tgatcgactc aaagcagaca gatcatagat
    13021 agcatttgct gttattgcca tttcttctgt agtcatatct ttggcattga taccaagctg
    13081 cgaaaatggt gaatccttcg actcaagaac cgttgactct gcaacgttat agtttagctg
    13141 tgccaccgtc attgcctttc tatagtagcg tctggcaggc ataactccac ttgcagagat
    13201 agagctgttc tcaatattgt tctggctata tgcaacataa gacgccgcat ttacgcctat
    13261 accggttttt gtatccccgt ccttacgctc aggaaactga tctatgatcc ctgcagtacc
    13321 ataagttacg ctgatatcag ccttgacagt tatagaacca tttgtgtcac tattgatata
    13381 atcgtagacc gaatcgggat acatcagcat atagctgtct ttagcttctg aaagtgtctc
    13441 cgtcttgctt atttttgcgt ttgataactc tgtgtctgaa ctgtttaaaa tgctgtaatc
    13501 aacattcacc gaagttcctg caatgatctt tgcattagct cccgcatcgt tttcatcaaa
    13561 gttcttcata gagaatttaa acgcctgata catattgaaa tcgtcactct tatagccatt
    13621 aaatgtatct ctaaggctcg gatcaatgct tatctttgac gtcattgtcg cacgaacaaa
    13681 attatttgac gctgttatct cctctgggtc atgagccgtc acacttacta gctgtgtaaa
    13741 aaagtttgca ataacatatg cgccagtatc attatttgtc acttggacaa gatttgtagg
    13801 aatattgcca ttgagctttc ttggcttatt accgctataa taattcacaa agttcttgat
    13861 gacctttttt gaaccttcat tttccggaat gatgatagtg agataatagc tttcgcttat
    13921 tatcatttcg tcattatcat ttttaggtgt atcactattc gctgaaacag ttatcttata
    13981 agcgcctgtt tcattctcac cagcaggtct ataatacttg ccgtcgcttg tttttacagt
    14041 agctgtagct tcatcagctg cctcaacaag tgttccgtca gatgattctt tagctgttac
    14101 cgacgcatat cttaacagta catcattcat tgtaaccggt ttaaagccac ttatatttgt
    14161 caagtcaagc tcaccggttg ttttattaaa cttagcatca cttgcggtag aatgataagt
    14221 cttgtcattg ttatttgcat caacaagagt tagctttgta tcatcggtaa gcacaccact
    14281 gtcagtggca ctatcaccaa taagataaag ctttttatca aagctccaaa gcagactgtc
    14341 accattatta agcatttttt cccattcgtt agcggatttg taataggaat acttaaaata
    14401 agtagtaaca ggtgcatcaa agctctcaaa agcaagcttt gttttatccg tatagtgaga
    14461 atggttatag tctgtgcctg agataacgta agattggaag ctgaaatcaa gcacctttct
    14521 tacaaaaaca ggaatatgaa gtctaagagc agttttgtca cttcccgttg ggtcgatata
    14581 atcaagagta atgacagtaa atctgtttgt gccgtcattg tcatactgac cgtctgtgac
    14641 cttgaaatat cctgtcttgc tgttaaacgt aagagtgctt ttgtctgatt ttttcaaaac
    14701 gccgttatca tacacataag tcgctgtgct tacattcata agatctgtta ttttcagctt
    14761 atttgaactg ctgtcgcaaa catcacaatt tgtcagaact gagatatact ttgcgagcat
    14821 ttgtgtaata ttcaacgatg agttatcatc tacaagcaaa actggaagat cgttcagctc
    14881 ctgaacatca agctcagagg cctgacgaaa tgttgtaagc tttgatttat ccaaattctc
    14941 tgcatatgtc tttatagtac tgtttatgcc ataggatttt gtctttgtgc cattcgtata
    15001 agaagcagcg tcactaatga tagtctgcat atttccgcca acaagatcac ctgcgaaagt
    15061 cttaccgccg acagtaacgc ttgggtttat attgacataa ggtgaataga tatcaacgtg
    15121 tgaactgctt ccatcaccga tattttgagt attattctga tcgccgttat agtcagcatg
    15181 aactgcgata aagttggtag gtatctgtga agcattatac tgtatatcag gtaaacaatc
    15241 cgtgttattt accgatactc caataaattt agatgataga tttttatcat tgttccaacc
    15301 tatcaagtta gaaacagaca cgttttcgtt gcctttttta tagcctaatt tatttatgag
    15361 aatatcataa gcatatgtgt ttccacggtc attatgaccg atcacgccgc ctgcacctgc
    15421 accattggac ttatcttctg ctgacagagt aacattgttg acttcacaat cagatatctt
    15481 cagatcacca ttagctttta taccaacata acctccgata cctgcatcac cagttttagc
    15541 ggttggtata cctatcgttg cattttttac agcagacttt gatatagtca cagccgcatt
    15601 ctgtgagccg accattccgc ctgaacttgt atagccataa acaccgcaag ctgatgtttc
    15661 agatgtttct ccatagtaac agtcattgat agacaactgg tttttgttta ttccaacgaa
    15721 tccaccggca ttactaccat aaacatcgac gctgacgctt gtttttgtta ttgtgcatgc
    15781 tccgttagac agtcctataa gaccgccctc gttcattgtc tgggttgcaa ggggtttatt
    15841 gtcttttttt gaaccgataa agctgtcttt attataggct gtaagctgaa cattgcttat
    15901 tgtcgttttg atatttgcag aacctgcgat tccacctacg ccaaaccaag tttttgtgcc
    15961 tttatcaaga ttagcaaatt caactttatt aatttttata ttagaattat caacagagaa
    16021 ttcgttgcct ttctgagaat ttcccacaag accgcctgtt tcaaatccgc catacacata
    16081 aacgccactg ttttcagact taacgttact tatattgata tcatttgttg actttcctat
    16141 aagtcctccc acagtatatg cgccatatat ttcaagatca gtcaatgtta ttccgataaa
    16201 cttgcaagaa ttctgtactc cgccaacgat accacctgta gaaaggtctt cattaacata
    16261 cgattgtcca tcgtagttat acgtcttaac gctgatcttg ccggaaagct taaggttatt
    16321 tacagttaaa gcatacgttg aacagtttat caacccaact ccttttacgg aggcaaatgc
    16381 ggtacgatat ttatagttat ccacatcttg gaattcaata gtgctaccgc tcttgttatc
    16441 cgttattttc acagcatagg cttttatatc tgtatcgagc tttatagtct taccatttcc
    16501 ctggatagct gtcaaagata aagtataatc acatttaatg tcgccaaggt tactatcctt
    16561 tgcatcaaac aactgattta taccacgaaa accggtttcg gtgaggtcat aggttttatt
    16621 tcctgtgaga ttaacagtaa agttcttatt taattcgtgt gcccactttg cttcataaag
    16681 acctttttca cttggctttg tatatttttt tagcattaca gaatttttct tttcatattc
    16741 cctgcttgta gccttttcaa gtctttgata atctgagatc gcagttttgt aatctttatc
    16801 atcagaagtc aatgtagctg tgccaacttt tgaatagtct gcatttcgag taaaagtata
    16861 atgaccatat ccgcaagtat tgtttctacg gtctgtatat cccataccgg actgtgatat
    16921 gatagacagc ataaacaacg cctgtgcatt aggcacttca atggtattgg tcgtgccggc
    16981 aataacattt aatttctcag catcattaag ctcgctcttg aactgcgtga tgagatagtt
    17041 tttcctgcca ttgttaaggt tggttgactt accgaacgtt tttccctctt caatagcaaa
    17101 gccgtttaca actctgccta tatatggatt gataaagagg ttggtagctg cgttttcgcc
    17161 cactgcctca gtattgctgg tcgtaagcgc actatctttt gcaacgttgc ccatatttct
    17221 gaaaataacg ccgccgtaaa cgatagcacc aacatatccg cctgcagtaa tgagatgttg
    17281 tttgctgtta tcgttatttg caaatgttat attaggattt gttaccttga cattatcaat
    17341 gatattatct ccgccaaaca caacgcccat aacaccgcca taatactctg tctttccggt
    17401 actatagttt aatttattat tattgttttt tgaaagcgtt acttctttgg tatattttat
    17461 attgatatct tttacaacac ttccgctgct gaacctgatg agcgggcttg cagaattatt
    17521 ggtgatagtt ggatatgttc cgtcggattt cttctgtcca acaataacgc ccctgaaaac
    17581 gaaactgttt gatgttccgc caagacctgc aaaagaactg ccgagaacta tatcatcatt
    17641 tatcttgtaa taagcctcgc aaagatattt gatcatgtta tcttttgaaa ctttttcctt
    17701 tccgcttacg aaattgcctg tttcatcata agtataagtt ttatgattat tgcctttaca
    17761 aaaagcacta ttagcattaa cagtcgattt atcggcagaa acatttgcat tatagtttac
    17821 ttcccagcca tttgtgggtg cggctgtact gataactcgt gcgacctctg caagtgtaga
    17881 agcgtcaagg atatatggat cagagtatgt tccgcaaccc tcgtccagat atggtcgctc
    17941 aaggtttacg tctatctcat catagttttg ggcggtatca tacgatttag caacatatgg
    18001 cttgtattca gtaaagctat attcctcttt tgcgttattt ttgtcaggag aagtatacct
    18061 gtcatccata ctccaatcac catgatattt attctgcctg ctatttccat aatcatcaac
    18121 attcttcttg gtttcagaca cttcttttcc gtaggttaca ttatgcttaa tattgccagc
    18181 tgagtctgta ccccaatcgt cgtcccattt atagttgtat attgcagaag agcctgcacc
    18241 gtcagaatgc tgaaagcttt caagcaaagt agcatttttg aaaattgaat ttttattact
    18301 tgcgtcaagc ttgatatttg agaaagtcag agatatattt tgaccctttt tgcttcctac
    18361 atcgcctata aggcttgttg cagcataatc ctgaccgcct ttatcatatt tagcagtagt
    18421 catactgtgt tttttctgtg acacattctg tatagtgatc tctgtcatgt tgcctatctt
    18481 atttataagc aaaggggcat aagaattttc accatttaaa cttaaagaag tatcattcac
    18541 atacagatca tcaagaacga tactgccggt aattttgaca gatttttttg aagtattggt
    18601 gtcgtcagca accgagccgc aaaccaatgc acctgaacca ccattcacct taccgatatt
    18661 tcccttaaag gtcagtttac cacttatggt aacagctcca ttttcattcc tgaaaagtcc
    18721 gcattgcatc atgtaatgtt gattatggtc attagttaaa tttgttccat ctattccgtc
    18781 agttgtacgg gcataattgt cactattact gcttgatacc atttcagact gattaaagcc
    18841 attattttca aaagtaatag ttgaatttga ttttatatta caaccatttg tatcaactgg
    18901 ataaaatgaa tatcctgtca aatctatttc tggatctgtt ggaaaatcta tatttgtact
    18961 attaatatat gcttttatat tatttgccgc aaaaagcttt gcgctccact caacagcctt
    19021 agctccacct gtggtggctt taccgttttt atatacatca aggttataat aatatcttgc
    19081 ccaagaattc tttttccaca cagcaccatt atttgttgtt tgattttgat aggtattgca
    19141 gttctttccg tccatataca gcaaacgctc tccgtccgct gtaacagcag gaattgaaat
    19201 tatggcttga cgattagaca taaaactagc tgaagaagaa taataaatac tacaacgagc
    19261 tatctcatca aaatacgaat agcttgggct tatatttatc ttagtatctt gtgagatctt
    19321 atatccatta ggtttagtaa gttcaaaata agttgcatct cgtgagcttc tgtaattatt
    19381 gacattttca cctttaaaat agtcaaagcc ataagagtca taatctctac caaaaagagt
    19441 tgaggcaagc atgccgaaat ttatacattt tgttgctttc actgtcacac cgtcaaagct
    19501 aacctctttt atgctccaat aacctgttgt ggaaagtaca agaccgccca gttctgtgcc
    19561 attgttcact gttaatcttg aattgtttac attcaaacta ttcagatcaa tttctactct
    19621 ataccagtta tgccctaaaa atccaccgct atttgattta ccactagaat ttattgttaa
    19681 agcattaata ttaacattgg tgatagatat cttattaggt acaacagaag ctgaattatc
    19741 cttagcaccg acctnnnnnn nnnntgtaac agcgcctacc attccgataa ctccgccaag
    19801 acaagtgcaa tctaccttgg ttgtgctatt attgtatgta agatttgctg ttacagcaga
    19861 attagtgaac gaaacttcac ttgtggcgtc agcaacatat ccaaccagac ctccgacaaa
    19921 attagtatac gcaccacttg gagatgcggt cacattggcg gtcacgctgt caatagtcaa
    19981 agctccgctg ttgtatgcag atatagaacc aatataacaa gcatcaccgc cagaaacatt
    20041 atcaccgaca atattaaagt ttgaaacaag cattaaattg cttatttttg caccgcttgt
    20101 ctttgcaaaa agtccgttgt gagtatggaa aactatttta gcgtctttgc ctacgctcat
    20161 agtaattgta tgactattgc cattcagagt gcctgtaaat gtatcctcac cattatcacg
    20221 cataaagccg gttagacctg tgtcgcttat agcaacatct gcactaaggc tgatatttgc
    20281 cgcaagcata tctgccctgc tagcaccgct atacttaaca aaatcattgc tttcatgctg
    20341 cattatcaaa gctgcacggg caaaatctgc cctattgctt atggtaatat tattattaga
    20401 aaatccattt attgtaacag tatgtcctga tccatcaaaa gacagaacac cgccagcact
    20461 ctcaagcaga tcggaattgt taaggcgaag cactccgccc caatccatat cgtctatgac
    20521 ttttgaagat gtccttgcaa atgaccagcc gcttaagcta tagatcaaag cattgcctct
    20581 atttcctact atctgacatc cgtttttgtt agggtcttta ggataaaagc ctgaaaggtt
    20641 tgtttcaccg ccaagcctta caacgccatt tttattaaat ttgccaacaa taccacctac
    20701 gctctggttt gcagaaacat tatttgcagt aactgtgact ttgccgccaa catctataaa
    20761 agcctgatct gcatagccca caagtccgcc ataattattt tgtgatgatg tgggattatt
    20821 aatacgtata gtggtattct tgacgcttac ataagccttg ctgttatcac ctatcttacc
    20881 aataatgccg ccaaaatcaa gggcattgca tgagccagtc acatttacaa tgatatcact
    20941 aagtgctagt tcagagctaa gagcattggc agaatatctt ccgacaacac caccataaaa
    21001 gcctgccctg acagtgccat cgaagtttga agtaatagta tcattagcgg tgcccgtgat
    21061 actaattttt gcactatctg tactatttgt cagcagtccg aaaacagaac caacagccgc
    21121 actgtcagcg gtatctcctg atgaacaagc aagtgccatt ttcatgccac tgaattttga
    21181 aatatcaaat gtcttttcgt tggctttact gtatgtataa gagccgaaaa gaccgcccac
    21241 agttacagag cctgtaacgc ttccggtcat ggtaagggtg acaccttcgc cgacatttat
    21301 ttctgcattc tctgcactgc ccacaagtcc gcctgcgttg tttgcggata catttacgcc
    21361 tgtcaagaca tcacacttgt caacgttcag tgttgcacca gcactcattt ttccgacaaa
    21421 tacacctgcg ttcgatttac cggaaatgtc aagcaaatta cttgacaagc ttacggcaag
    21481 acttgcgttt tcgtccatag tgccacaagc aagacctgca ttatccccgc ctgatacttc
    21541 cacctggaca ccattactta gagtgatatc cagatcgacc tttgctccat ttttcatatt
    21601 gcctataaca gacgtgaagg atttataatt tcttgcacct gaatcatcta caggatcagc
    21661 ttttattttc cacttattag ctgaatccac atcaccgtgt ataacattct cagcaagcaa
    21721 agctgaattt tttcttccgg tctagcaaaa ataatagtat ctaaatttgc actgtcacta
    21781 agatattcaa acagagcaaa attaatagga agattaatag ctgaaccctc atttgctttg
    21841 accgttccca tgaaaggata ttcttcattg ccaagtcctt tttctattcc tgtaaaatca
    21901 gaagccttaa actgagattg gttgtttgaa aacaatactg tgattttctg atagacagca
    21961 ggatctgcat taagaagctt tttaaaatcg tctgcattct gtatggtata aacgccattt
    22021 ttgatatcat tagttatgtc agtatatgta tcttccgcca tagccgtaat agcgttggtg
    22081 acagttgata ccattttaga cacaacacct gaaatgtctg caagcggcat actcgtgaca
    22141 agcaatacgg cagcggtaac taaagaaata atattcttgc gatacttgct gtaaagcttg
    22201 cggcatattc tattaatttt ctgatttcta ttagctttca ctttcaactg cctccgtgca
    22261 aagttaatat tcttatctat acgtttagtt caacaaggat atattgcacc tttgtaccta
    22321 tcttttcgtc agcgtcatta tacatggagc ttagtgatga taactgtgct gtgctgtatt
    22381 tatcaccatt aagtatattc tcaataacct ttttattttg ttctttatcg gcatcacctg
    22441 acaagaacaa gcttaaatta cccttcatat attccgcaaa attcaaacgt tctttactgt
    22501 catcatcaaa cagcacgttt ttttctgtca gattttcatc tgtgcttttc attagtatct
    22561 gaccaagcgt gttcggccac acccaatata cgttcacttc ctgcggaacg cctactttac
    22621 aatctttaag cgtccttgtg aatgtttcat catgaagaaa atcggaatat gtgtgattgt
    22681 tctcatcata tttctcaaaa aacatgatat gtccattcat aaatctagtt acttcttctt
    22741 cagtaacctt ttcataagaa acagcgtctt cgtttttatt ctctgcatac cccgtgatac
    22801 tcaacttgaa ctgtatctcc atttctcccg actggtttgg gacaacataa aaagtaagct
    22861 ttccatgact accaggatta atacctttcg tcataccgct ttcttcatca agaagccagt
    22921 agatcttgtt gttctcgtca gaagtgatgt tgctcccatc agacacctta gagtagtcat
    22981 tgctgtcaag tatctcagct ttaagcacat tggcactgcc tactgttttc agttcaaatg
    23041 gcgaatctac gcttttcatt ccaaatgaat caagatcaag ggctttatca atggtaaacc
    23101 aagccaagct gaccgcaata agtataaatg ccagcactgc aactggtatt atttttataa
    23161 taagcagttt gcgtttcttc ttttcttcct cgctgtctgc tttctctatc actttatgct
    23221 cagccacttt ggcgacctcc attatttcat cgagtcaagc tctttctgca attctatcat
    23281 gtggtttctg caagcgatta tctcgcctgt gtatttgttg aaccactcaa cgtccatttc
    23341 gttaggctct tcctgcaaaa gaattgcctg aacgagtgct gattgtgaac gtgatctctt
    23401 acgctctatt tcctcgatag taagtctgca tttctttatt tctcttttaa gctgcccttt
    23461 actgctcacg ttattgtcct ccttgcatta atttcccatt ttatcaaccg aatcaactat
    23521 ctcgttgact tccctatagc ctttgtcaag attttcggtg atatggtcaa gtgctttttt
    23581 tacgtcctca agcttgccct gcatttgttc gggatacttc acaaagcagt aatgaagctc
    23641 atttatagcc ttctgctcat cagtataatc gtcgataaga aataccagcg accttgccgc
    23701 acccatgtcc gaaagtatca tgttatatct cgttggaaga tccttgccgt tttcaagata
    23761 agtgtctatc tcctcagagg cggcagacag attttccata tacttagcaa tgtaagtctt
    23821 tctgacttcc tcggacttgt ccatataaag cccgataaaa acgaacagcc ctgcaagtgc
    23881 cgcaatacaa ataacgatag ccgttatcat tttagccttc attatctttt cctgattcaa
    23941 agctgttcgc ctcctttttc tcgttttcat gcattttcag ccgctgaact tccttttcaa
    24001 cagcctcacg cataagcttt tctttttctt cctcagccgc tttcaaagct tcctcacggc
    24061 ttgagtatat ctttatcttt gcgatagttt ttacctcata caccgccatg caaactgttg
    24121 ggattatcac aaacagcatg aggagcttct ttgttgatgc aaatgaccca agccacttga
    24181 aaaatctgag ctttccatag tattttccaa taatgctggc tttttgcacc gtcacgctat
    24241 cctctatttt gttagcatcg cctttagtaa caaaagtgcc gtcgctcaaa acctcagata
    24301 tcctgtgagt atttatctcg cctttaatac tatcatcttg agagtagaac gaaatgatat
    24361 cccctgcctt taagctgtca gtatccgtct ttttgatgta aatatagtca ccctcatgta
    24421 tagacggctc catacttccg ctcacgacct ttagtacgct gtaaccaaaa atgcttgctg
    24481 ttttgccgat acagccgcag accattatgt acaccgtcac actcagcagt gccgcaaaca
    24541 cagcagagcc aagtattgtc aaagctattt ttactatcct caacgccttg ccgatgataa
    24601 cgactctccc tccgtgttat tgttttctat atttctcttt caaatgcctt gccattctgc
    24661 caactgctga aacatgatac tccttataga gtcgtttcat ttcagtgtcg atagctttct
    24721 gctcctcagc ggtgcgctgt ggcggaatat atggtcttgg cggtctgttt tttcgctcaa
    24781 gacgttttct ctttttaaac tgcctgtttt tgatacgcct ctttacatca tatttcagat
    24841 acggagcata tttttcatag ataacctcga tttcacgctc tgcacgtttt ctctctgcct
    24901 cagccagttc tttttccttc ttgactttga acattctgat atagtccgga ttatccgtca
    24961 caacaggtat gatcccaaat gcgtgagcca gaacatatgt tatgccacgt tttgccatgt
    25021 aatatggatt tttggaatat tctttaaata tctcattgat atttttattt tcaaagcttt
    25081 cacgttcagc tttaagacgc tcagcacgtt cacgctcacg acgttcctgc tcacgctgct
    25141 gttgtgcttc tttcgccttt tttgctgcga tctcagggga ctcatactct gacacaaagc
    25201 ttgaagacac ggcttcttca ttcgtgctga tctgctgatc catagcagat ccaaggctgc
    25261 tgcggacgag ctttttctcc tctcggatcc tagctctttc ctcacggaca cgctgctcct
    25321 cttcacggcg ttgacgctct tccatttgcc ttttgcgtct tgcaatatgc ttgatct
//
LOCUS       DAWEMR010000011        24967 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_806_length_24967_cov_11.234104, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000011 DAWEMR010000000
VERSION     DAWEMR010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 24967)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24967)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24967
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_806_length_24967_cov_11.234104"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6152..6161
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16370..16379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6707 a         5348 c         5553 g         7339 t
ORIGIN      
        1 tatgaataag ccttaatcca ttgcaaatat tgccactcct gccattactg ccacaagcag
       61 caccgcagcg aatgagatat agaaaagcag tatgagcctg cccttgcttt tctctttaac
      121 gtcccacttt ttcgagggga tactaggtct tgtcacaccc attattatgg gcatgattag
      181 cagatatcca agcataccga gtataagatt tatgctcggc gtgtcgtcca tattcttcat
      241 aagcagaaaa ataggtatca caataagaat gtgcgccagc gccatgaata cacgccatat
      301 cctgcgctgt tttatcttcc tgtcccttat gccgttgagt tcttcctccg aagcctttct
      361 ctttagaaat tcctcctgcc tgagccttac ttcttcaagc ttcgcctcat caactatctt
      421 gaccgtccta gtgccgtcgt caatgtagtt ctgagtgccg cagtaagggc agtagacgat
      481 ctttttgtcg tcctctatcg ttatttcacc gccgcagttt ttgcactgaa gtgcgataag
      541 ttccaacttg ctccccccct gactatttga tgaaacgtaa gtaaagcttg cccactccga
      601 agaatatcgc agtgaacgtg atatagaaaa gcaccgcagt ttttatcctg cccatgcccc
      661 ctgcgtttct cggcttaata agcgccatta tcacaggcag tgccacccat aagcatatgg
      721 aagcgattat tgccagcact gacttataca tgaccgcacc gctgaacaca agcgtgaata
      781 caacgtgcaa aacaatgcaa acgattatcc acccctgctt ctgggaagcg tttttgtatc
      841 tgccgctcgc ttcctcgtca cgaagcttct tgtcaaagtc cgatacctct ttgttgagct
      901 ttatctcttt gagcctagcc tcgtcaatga cgtttattat cctgtttccg tcatcaagaa
      961 gtatctgtga cccgcagtat ttgcagaacg tctttttctg cccctcgtga acttcaagct
     1021 cgccgccgca tttgggacat tttaatgcaa ctaactccat tttttctata ctcctgcttc
     1081 gctttaggaa attacttgct gtacttgttt ataaccttag aaacagcctt gccgcttgcg
     1141 atatcaatgt atctaacaaa cagcgatacc ttgttgtcct cgttgagtgc ctcccaaagc
     1201 ttgcccgtga actcttcgat atcgtttggt atctccacct tcttaggctc gccctcaaag
     1261 cttggcagag gattgccgtc acccatgtaa gtgtggataa aatgacccac gccgttaaga
     1321 gggttggtgt atgtgtaagt aactctctcg cagcagtcag ggttgccgtc agcggacttg
     1381 agtatgctca tagcataatt atatgcgccg ttctcaacgt gcatgatacc tgaaatacga
     1441 ggagtgtagt ttggagcgtc gtcctcatac tctctcgtcc tcagagattg ctcaaaagtc
     1501 tgctgcttgt ccataagctc gtaaatagtg tccgtctgat cgccgtttgt aacgatagtc
     1561 ttgtttccaa gcacacgaac aggggagtat ataataaggt gtgggtcaga aagcttgcta
     1621 gggtcagcag cttctgtctt gatgccgtcc tcagtttccg tgaaaactct gtttctggaa
     1681 ttaacacttc tgcccatgat aaaataagcc gttaccgcac tctttccgtc agcagacttg
     1741 cctataacta ttcctctgcc cggatatgag ttgcttttaa gctcgtcata aatattaagg
     1801 gtcttcatat cttgtcctcc ataatttttc tcagtcttta aggtcaacgt aagccttgcc
     1861 gtccttaatg cttatcctgt cttcgcagaa cagcgaaacc gccttaggca aaagcttcca
     1921 ttcaacgttt tccataatct tcttctgtaa gctttcaggc gtttcgtcat tagctacgtc
     1981 cacagcgccc tgcaaaataa tagctccggc gtcagccttt tcgtttacaa aatgtatagt
     2041 cgcacccgat accttaacgc cgtattccaa agccttttca tgaactttaa gcccataaaa
     2101 accctcgccg cagaatgacg gtatcagtgc agggtgaacg tttattatcc tgtaaggata
     2161 agcctttgtg acgcattcgt caagaattat cataaatcca gccagcacga taaggtcagc
     2221 accttgtctg ttaagctcag ccagaatagc ctcgctgtaa ctcttgttgt cagcatattc
     2281 cttcctcgga ataacgacag tgccgatacc tgcatttttc gccctctcca gagcataagc
     2341 accctccttc gaggatataa cgcaggttat cttgccgttt tttataatgc cgcttttttc
     2401 agcgtcgata agtgcctgta agtttgttcc gccgcccgaa acaagaacgc atatcttctt
     2461 catatctctt tacccttagc agatgataac gcccatgtca gagtcaacaa gctcgcccag
     2521 aacgtaagct tcttcgcctg ccgccttgat agcttcgata gccttgtcag cgtcagcctt
     2581 atcaacaaca acagtcatac caacgcccat gttgaatgtg ttaaacatat ctctctcagg
     2641 aatgtttcct gtctttgcga taacgtcgaa aataggaagc acctgaacat cgcttcttgc
     2701 tatcttaaca gaaagaccgc ttggaagtga acgtggaatg ttctcataga atccaccgcc
     2761 tgtgatgtgt gatatagcct ttaccttaac agaatcaagc aggctcataa cagcctttac
     2821 atatatcttt gtaggtgtga gaagtgtctc gccaagtgtc ttgccaagct cgtcatagtg
     2881 aacggaaaga gtcttttcgt caacgctgaa tacctttctt accagtgaga agccgtttga
     2941 gtgaacacca cttgacttga tagctatcat aacgtccccg gccttctgag tgcttgggtc
     3001 aagtatcttg tccttgtcaa caacgccaac agagaagcct gcaaggtcat attcgtcaac
     3061 agggtagaaa ccaggcatct cagccgtttc gccaccgata agcgcacagc ctgactgtac
     3121 acagccctct gcaacacctg aaactatctg agctatcttc tcaggatagt tcttgccaac
     3181 agcgatgtag tcaaggaaaa actgtggctt tgcaccgcag cagataatgt cgtttacgca
     3241 cattgccacg cagtcgatac caactgtgtc atgcttgtcc ataagaaaag ctatcttgag
     3301 ctttgtgcca acgccgtctg tgcctgaaac gagaacaggc tttgtgatac ctgtcatgtc
     3361 aagctcaaac agaccgccga agccgccgat gccgctgaga acaccgctcg tcatggtcct
     3421 tgcaacgtgc tgcttcataa gctcaactgc cttgtagcct gccgttacgt caacgcctgc
     3481 ttccttgtag ctgtttgaaa aactgtttgt attcatgata aaatatcctt tcagttattt
     3541 acttcttttc cgatatcttc gattcaaatt tgtccttagg catatcctta ggaacttcga
     3601 tagggtattt ctctgtaaag cagcctacgc agaaatcaca ctttgaattt cccgcaagct
     3661 ttttaacgct ttcaacactg agatatccca agctgtctgc gccgatatat tcgcatattt
     3721 cttcattggt catcttgcag gcgataagat tttccttgct gtctatatcc gttccgaaat
     3781 agcatggtgc aatgaatgca ggggaggaga tacgaacgtg aacttccttt gcacctgcgt
     3841 ccctcagcag cttgactata cgtccagaag tcgttcctct gacaatgctg tcgtctatca
     3901 taacgaccct cttgccctta acagtgcttg aaatagcgtt gagctttatc ttgacggagt
     3961 ttgtcctctc tgtctggcta ggctgaatga acgtcctgcc tatgtatctg ttcttgataa
     4021 atccaatgcc gtatggtatg ccgctttcct gagaaaatcc cagcgcagcg tcaagtcccg
     4081 agtcaggcac gcctatgacg atatcagcgt caacagggtg ttctctccaa agatattttc
     4141 ctgccctgag ccttgccatg tgaacgctcg acccctcgat aacggagtca ggtctggcaa
     4201 aatatacata ttcaaatacg catatagtac cctttttacc acagtgagtc gtgatagagt
     4261 tcacaccgtt tttgtcaata actatcattt cacctggttt aagatctctt atgtattcag
     4321 cgccgatgct gtcaaaagca caggtctccg acgctaccac atagccgtcg tctatctttc
     4381 cgaggcaaag gggtctgaag ccgtcagggt ctctcgccgc aataagcttt gtcggcgaca
     4441 taacgagcag tgaatacgca ccctttatcc tgtacatagc cttttcaagc gccgcctgta
     4501 tagacggtgc tctaagacgt tcctcggtta tggcgtatga tatgacctca gtatcatttg
     4561 tgctgtggaa tatcatgccc ttgttttcat attgctctct aagttctctt gcgttagtaa
     4621 gatttccgtt gtgagcgata gccataggac cctttatgtg acgaacaaca agcggctgag
     4681 cgtttgaagc attgcagttg cctgtggtcg aatatctcac atgacctata gcaatattgc
     4741 ccttgccgag ccgttcgaga gtttccttgt caaaaacctg attgacaaga cccacgtcct
     4801 tgtgatagct gaacacgccg tcgtcgttta caacgatacc gcaactttcc tgacctctgt
     4861 gctgtaatgc gtaaagagat acataagtct gagctgcaac atcgcttgtc ttgttagtat
     4921 aaatgccgaa aacgccgcat tcttcatgga tacctgttac caattaaatc attcccttcc
     4981 gttccagcag taggtgcaaa gctcacagct tggcagaccc acagccttta cagtgccttc
     5041 aagcgactgg aattcaacag aggtaagttt aagtctcctg catatctcgt ccctgaggag
     5101 cttgccacgc tcagtgcttg aatcgctgta ttcttcaatg tatttaactc cctcgatacc
     5161 ctcgttctca tcaatgatct gtcttgcgat aagttcaagc tccgaggtgc tgcgtgaaaa
     5221 gttaagatac ttgcagccat acatgatagg aggacaggct gatctcatgt ggacttcctt
     5281 tgcaccgttc tcataaagaa actcaacagt ttccctcatc tgagttcctc tgacgatact
     5341 gtcgtcaacg aacagaagct tcttgccctc gataagttcg tgaacaggta taagcttcat
     5401 ctttgcaact ctgtttctca tagactggct tgttggcata aagcttctgg gccatgtagg
     5461 agtgtacttg atgaacggtc ttgcaaaagg tatgccgctt ttgtttgcat atcctatagc
     5521 gtgaggaacg cccgaatcag gtattccgca aacgaaatca acgtcaggga gcctgccgtt
     5581 tttcatatcg ttctccgcca ttatctcgcc gttgcgtgtt cgcataagtt caacgttcac
     5641 gccctcatag caagcggtag ggtagccgta ataagtccaa aggaaagtgc agatcttttt
     5701 cttgtctgta ccctcgctga ggatattgta gccgtccttg gttatctcag ttatctctcc
     5761 cgcctttagt tcgtgacagg tggagtatcc aagcttctga tatgcaaatg attcaaaaga
     5821 aaagcaatat ccgtcagaac gctgtcctat tataatagga agccgtccgt ccttatctct
     5881 ggcaacgatc atcgacttgt ctgtcattat tgcaagtgac atcgtgcctt ctatcttgtc
     5941 ctgtgcgtat cttatgccgt caacaaagct gtccttctgt gcgatcagcg caccgataag
     6001 ccctgcggaa ttgatagcgc cgctgctcat tgtttcaaag tttgcgcagc ccttttcaag
     6061 cagttcgtca gccagtttag cggcattttg tattacaccg atagagcaca ctgcgtaaag
     6121 tcctaacttt gagcgtacca ttatcggctg annnnnnnnn ntgtgtcgct tatgcagccg
     6181 atacagatgt ttcctctcat tttgtcaacg tctttttcaa atttagtacg aaatggtgag
     6241 ttttcaatgc tgtgaatatt tctctgaaag ccaagctcag gggagaatga agtcattccg
     6301 ccccgccttg tgcctaagtg tgaatgatag tcggtcccga agaaaacgtc ctgaatacag
     6361 tcattagctg aaacagctcc gaaaaaaccg cccatagata ttattctccc attactctct
     6421 ttatcatctc atggtaagcc tcttcaacgc cgcccatgtc tcttctgaat ctgtccttgt
     6481 cgagcttctc atgtgtctta gaatcccaga aacggcaagt atcaggtgag atctcgtcag
     6541 caagaagtat ctttccgtgg aatctgccga actcgatctt gaagtctata aggtcgatgc
     6601 cgatgttctt gaagaagcca agcatgaact cgtttacctt gaaagcatac tttgtgatat
     6661 cctcgatctc ttcctcagta gcaagaccaa gaccaagagc ataatacttg ttgatgaatg
     6721 ggtcgccaag ctcgtcgcac ttgtagctga actcaagtgt aggctgcttg agctttgtgc
     6781 cttcctcgat accgagcttc tttgagaagc ttcctgctgc aacgtttctt acgataactt
     6841 ccagcggaac gatctcgacc ttcttaacga tagtctctct gtcagagatc tcctcaacaa
     6901 gatgagtagg gataccctct ttttcaagaa gcttgaacat atagtttgtc attttgttat
     6961 tgatagcacc cttgccaacg atagtgcctt tcttctcgcc gttgaaagca gtagcgtcgt
     7021 ccttgtaatc aacgataaca agatcagggt cgttagtagc gaatactttc ttagccttgc
     7081 cctcatagag ctgttctgtc tttacaatgt tttccataga tttattcctc cataaattta
     7141 tttacattat ttatatgata caccgaaaac atatccactg ccgttttaat atgtaggggc
     7201 gaacatcgtt cgcccgccga cagcagaact tccccgatat atcaaatata acatacagcc
     7261 gagctatatg tctgtatcaa aaaatcaaag cgcagcaagc ttgtcctgta aagccttgtc
     7321 cttagccaca acgccgtcga tcatatcctg cttagccttt tcaagcttct cagccagacc
     7381 ctcgtcagaa agagcaagca tctgaatagc aagtatagca gcgttcttag cgccgtcaac
     7441 tgcgacagtg gcaacaggaa taccactagg catctgaacg gtagcaagca gagcgtccat
     7501 gccgtcgaag ttagcaccct tgcaagggat accgataaca ggaagcgttg tgtgacctgc
     7561 aataactccc gcaagatgag ccgccattcc ggcagcgcaa attataactc cgaagccgtt
     7621 ttccttagcc ttgtcagcaa aatcgcttgc aagcgcaggc gttctgtgag cagacataac
     7681 gtgacattcg taaggaacac cgtaagcttt aagttcagca acagccttgc tgacaacagg
     7741 aaaatcgctg tcagaaccca ttattaccgc aacttttttc ataaatttat acctccaagt
     7801 aaaacaaaaa attacaaata aaaaactgca acaatggcgt tgcagtttta gtcagtgcat
     7861 aattatattg ttagagtgca aaaaatcaag gcaggacgca attatactta aagacctacc
     7921 cataaaagaa tatgtaattg atgaaaaaca gcacatttca gtcatacatc tctcctgaat
     7981 acatacaaaa aatactgcac aatattacca gacagtaaag cggagcaaaa ctgtctgcga
     8041 aagctttttt tactgatttt ctccataaca gcatacaaag ccttacaata atattgtaca
     8101 atatttatag ataaatttca acacatattt atataaaatt tatatcaatt aatctttgat
     8161 ttattgtgaa atttgtctac attaactttt tgttcagaaa atacttgtat cagtgatctg
     8221 accgtttgac aagctgttga cgagatcacg catttcctct ttggagcgaa tacagtgtgc
     8281 cgagtcgatt atctgctgtc tttgaagcgg atccagctta gcaaagtaag catatgccgc
     8341 aggcacctga gcaagagcca tagacagccc cagcggcaat tcctgcccgt gagcaaaaga
     8401 gttatttttc atttttatca cctccgcata tattatttcc cgggcaataa aaataataaa
     8461 cccttacgat cttatatgtg atactgcaca atttatcaca tcgtaatttg tgcaaacaaa
     8521 tgatttatct tttaatttgg caacatttct gcaattgttg cattttttat gcaacaaaag
     8581 tggtgttttt cgggataagt ataggggtat tatcccaaat tgaatatcct gcaaggtagc
     8641 agtgtatgag gaaacatttt gtcaacgtga gttaggtgaa cacgatgaga ggttctgatc
     8701 gcacagaaaa gcacttcctg caccttgttt gaagcttgaa aattgaatat aaaatacgga
     8761 taaacccacg gcaataaatc tgtaggggcg aacaccgttc gcccgtcgaa ggaacaagcc
     8821 cacaattaca acacggtttg gcaaccgaaa aacttgccca ccgcacttgt atgggagcga
     8881 cgcaatttct gtttcacaga aatgggcgtc ccgttcggag aggtaacgcc gtggttttgg
     8941 tatcttcgat gttgaattat tcggggtgac attgttgtga taatctccgt taattcaatt
     9001 ggtgaggaat atcgggacgc cgagggcgtc gtcccctaca attatggata ctgttgatac
     9061 gcatacctcc attgaataca tttatcccca cccgaaaatc tgatttcaga cctgcaatac
     9121 cccttaataa attgtacaat atgcttctga aaccaatttt tatcctgata gatattgtgc
     9181 aatcctacaa aaatcacata aatgacctta aatgacttga attttgcgaa aaaggtgcta
     9241 aaatataatt agcactcaga aagactgagt gctaacaatc aaacggtgag agtgctgaaa
     9301 agactaaaaa tacagtagct cacaatattt tacaaagctt ataattggta ttataaaagg
     9361 aggaacttat catggcgatc aaaccattgc aggacagagt tgtagttaag atgacagagg
     9421 ctgaggaaac cacaaagagc ggtatcattc tcacaggctc agctaaggaa aagcctgagg
     9481 tcgcagaggt aatagctgtg ggccctggta aaaaggacgt tactatgggc gttaataagg
     9541 gcgacaaggt gcttatctct aagtacagcg gtactaatgt taagctggac ggtgaagagt
     9601 tcattatcgt taaaatggac gacattctcg cagttgttga ctaatttcac tgcattttaa
     9661 atagtatata aacggaggta atttacaatg gctaaggata taatttttgg cgaagacgca
     9721 agaaaagctt tgcagagcgg tatcgacaag ctcgcaaaca ctgttaagat aacactggga
     9781 cctaagggca gaaatgtagt tcttgataag aagtacggtg ctcctcttat cacaaacgac
     9841 ggcgttacta tagctaagga gatcgagctt gacgacccat tcgagaacat gggcgcacag
     9901 cttgtaaagg aagtcgctac taagactaac gacgctgccg gtgacggtac aacaacagct
     9961 acattgcttg ctcaggctct tgttagagag ggtatgaaga atatcgcagc aggtgctaat
    10021 ccaatggtac ttaagaaagg tatggatcag gctgtagata ctgctgttga aactatcgtc
    10081 gctaactcaa agaagataga aggctctgat gagatagcta gagtaggtgc tgtttctgcc
    10141 gctgatgaga acattggtaa gcttatcgct gaggctatgg aaaaggtaac tgctgacggc
    10201 gttatcactc ttgaagagtc aaagactgct gaaacttaca gcgaagttgt tgagggtatg
    10261 cagttcgaca gaggatatat tgcaccttac atggtaacag atacagataa aatggaagct
    10321 gttcttgatg acgcttacat tcttatcaca gacaagaaga tctctaacat tcaggaaatt
    10381 cttccacttc ttgagcagat agtaaaggct ggcaagaagc ttcttatcat tgccgaggac
    10441 gttgagggcg aggctctttc aactcttatc gtaaacaagc tgagaggtac tttcacttgc
    10501 gtagctgtaa aggctcctgg ctttggcgac agaagaaagg aaatgcttca ggatatcgct
    10561 atccttacag gcggtcaggt aatttcatct gaacttggtc ttgaactcag cgaaacaact
    10621 atggagcagc tgggcagagc aagacaggta gtcgttcaga aagagaacac tatcattgtt
    10681 gacggcgcag gcaacagcga cgatatcaag gcaagagttg gtcagatcaa gtcacagatc
    10741 gagaacacaa cttctgattt cgacagagaa aagcttcagg aaagacttgc taagctttca
    10801 ggcggcgtag ctgttatcaa ggtcggtgct gcaactgagg tagaaatgaa agagaagaag
    10861 ctgaggatag aggacgctct ggcagctaca aaggcggctg ttgaagaggg tatcgtagca
    10921 ggcggcggaa cagctcttat caacgctatg ccagcagtta agaagctcgt tgacaagctc
    10981 agcggtgacg aaaagacagg cgctcagatc gtttacaagg ctcttgaaga accagttaga
    11041 cagatcgcag tgaacgcagg tcttgagggc agcgttatca tagacaagat aatccgttca
    11101 agaaaggtcg gctacggctt caacgcttat acttctgagt atgttgacat gatacctgca
    11161 ggtatcgttg accctactaa ggttacacgt tcagctctcc agaacgcagc ttctgttgct
    11221 tcaatggtgc tcactacaga gagccttgtt gctgacaaga aggacccaca ggctgacgct
    11281 gctatggcag ccgcagcagc aggtgcaggc ggaatgggcg gtatgtacta ataagctccc
    11341 atgactaata tctgatataa tttacagcct ccgtagttat cctgcggagg ctgtttgtat
    11401 ggaatgggta atattttatg atatagttta caaaaatcaa ataatatcct cgatactttt
    11461 gtaagtaaaa tccaaaagta ttgattaatt aaattaaata tatacatatt gttgcagacg
    11521 tccgcttaaa attgaagcaa attatgatat ttaataaaaa attaaataaa tattcacaca
    11581 atgggtttgt gaatttgtta taatacttat atcaatttga ataattattg ccttttgttc
    11641 aaattttcag aagtgcgaaa atttaaaata atgggcatga aaagaagtaa ataaacgaaa
    11701 ggaagatgaa atatgtcgct tggacgagta ttggtagtag acgatgataa gaacatctgc
    11761 gagctgctca ggctttacct tgaaaaggag ggctatggag taatacttgc acatgacggt
    11821 gaggaagcag tggtaaaatt caacgctctt aagcctgaca tcatactgct tgacattatg
    11881 ctgcctggta tagacggctg gcaggtatgc cgtgagataa gaaagaagtc caacgtgcct
    11941 atcatcatga taaccgcaaa ggtagaaaca tttgataagg tgcttggtct ggagcttgga
    12001 gctgacgact atatcgtaaa gcctttcgat acaaaggaag ttatcgcaag aataaaggct
    12061 gtttacagaa gagtaggtca gtcatcaggc gaagctgaga taaaggaagt caagtatgac
    12121 aagctttcag ttaatatgac aagatatgag ctgagagtaa acggtcaggt gcttgataca
    12181 ccgccaaagg agcttgagct tctgttccac cttgcttcaa atccgaacag agtttataca
    12241 agagatcagc tccttgatga ggtatggggc tttgaatatt acggcgattc gagaactatc
    12301 gacgttcacg ttaagcgtct gcgtgagaag cttgagggcg tttctgacaa gtgggcactt
    12361 aaaactgtat ggggcgtagg atacaagttt gaagtcgctg aaacaccgca tgagtaatcg
    12421 taaaagcata ttctttaaat actttgtaat ttgttcagca gtaatactta taagctttgt
    12481 ttgcttagga acggtattac tgctggtttc ttcaaggtat tttattgacg aaaaaaagag
    12541 cctgttggag aaaaacacca aggctcttgc tttatatacg caggaagaaa tgctggctca
    12601 gcctgccgac tggaaaaagt cggtgacaga tgagatgatg cagtattcaa tggcgtgcaa
    12661 cgctgatttt atcttgtgcg actctacggg agcagtgctt gtgaactcca gtaaggatcc
    12721 tgtgtgcagc cctcataata tatctgtcca tgagcttgaa gctttttctg acaattgcga
    12781 gtatgtttat tcggatctcg gaggagcttt cgatgctata tatcatgtga taggtaagtc
    12841 gttcaaagcg aatggacctg aatattatgt gcttgcattc agttcaaagg cggctcagga
    12901 cgactatacc tacaacataa tggaaatatt tatagtttcc gctataatag ttctggtcat
    12961 agtaatgttt ctggtatatt tcatgacgct gaagcttaca gagcctataa aagagatagc
    13021 ggaggtgtca aagaagatag gagcaggaga tttttctgtc accctgcctg attattctac
    13081 ctcggagctt gaacagctca gcaacgcttt caacgatatg gctgccagct tgaaaagcta
    13141 tgacaccatg cgaaatagct tccttgcgaa cgtttctcat gagcttagaa cgcctatgac
    13201 gtctataggc ggttttgtgg acggtctgct tgacggcacc ataccaaagg agcaggagag
    13261 atattatctt aagataattt cctctgaggt acacagactt gcaaggctcg taagaagtat
    13321 gctcaatctt gcaaagatcg aggcaggcga gcttaaaccg aatttgcagt atttctctgt
    13381 gcttgagccg atagtcgata cccttgtgac ttttgagcca aggcttgagg aaaagcagat
    13441 agagataagg ggtcttgacg ttgacagagt gatcctttat gcggataatg accttgtgca
    13501 tcaggtcatg tataacttga tagagaacgc cataaagttc gttgacaagg gcggttatat
    13561 agagtttcac tttgagcctg agggcaatat gacaagcatt tctatcagaa acagcggtga
    13621 gggtctgtct gaagacgagc ttccgctggt gtttgacaga ttttataaga ccgataagtc
    13681 aagaggactt gacaagacgg gcgttggatt gggacttaat atcgtgcgtt ctatcataaa
    13741 gctccacggg ggcaagataa tggtacgaag cgttaagaac gagtatacgg aatttgtgtt
    13801 cacgctgcct aacaaggcgg agggcgacta ggaggctcat atggagcagt ttgaagagca
    13861 gccttgtctg catgatgaaa atgaagatac agcccaagaa actgggcatt acaagctgcc
    13921 tgagggcgca gtagccgctc acaaggtgga cgaagatttt tggtcggctg ttgattcggc
    13981 aagaaaaaga agccgccgca gagagcgtat gcgccatgca gttgcaaggc tgtgctttat
    14041 ggtgcttttt gtaggcatcg gaatagccat atcgataacc tccataaggg gaagcggctg
    14101 ggtgagaaat cttgttacag gggacgagaa gataagcttt acgcttcctg tggctgacaa
    14161 gccgaagctt gacgatgaat tttatcaggc tgacggaaga tacactgccg agggtatcgc
    14221 aaaggggttt tctcccgctg tggtatctat agaggtttac aaaaattcgg gtggatatgt
    14281 tgcggcaggt cagggcagcg gtgttataat gtctgctgac ggatatatcg tcacaaacgc
    14341 tcatgttgta aatgcggcgg agtcgggcat aaaagttgtt ctaagcgaca attcggagta
    14401 tgaagccaag gtggtggggc aggaccctgc cacggatata gcggtgataa agatacaggc
    14461 gcagggcttg caggcggctg agttcggtga ttcaactcag gtgaatgcag gggaagaggt
    14521 agtcgctatc ggctcaccgg ctggctatta cggcactgtg acaaagggta tcgtgtcagg
    14581 tgtgaatcgc aggataaggc ttgaaaactc gtcaataata atgaactgca tacagataga
    14641 cgcagccata aaccccggta actcaggtgg agcgctgttc aatatgtggg ggcaggtcat
    14701 agggataacc tcatcgaagc ttgcttcaag cgactatgag ggcataggct ttgcggtatc
    14761 tatagacgag gcgaagcctg ttatcgagga gcttatggaa aagggctatg tggcaggcag
    14821 agtaaagata ggtgtgactt attatgctgt cagcgacacc actgctgaga tatacggcat
    14881 aaagccgggc atttgtattg tgtccataaa tcctgattgt gacgttgcca acacagacct
    14941 tgcggagggc gatattatca cggaaataga cggaaagtcc accgcaggag aggtggatat
    15001 agcgtcgctg ttttcaggaa agcaggcagg agatgaagtc gtttgcaagg tatatcgcaa
    15061 gacggacagc ggtgatgaga aagagtttga gataaaattc aagcttatgg cggataacgg
    15121 cggtcttgtt acagacagtc agaataaata aaaaaagaaa aaagctgtgg taagaaaggg
    15181 taccacggct tttttcttag gggattaata aaatatgtga tgatattata gaattactat
    15241 aatactatta ttatatattc ccattagttt catgtcaaca taaaagtgtg acagctattt
    15301 taaaaaatct gtcacacttt ttgtcactgg aaagatgtat aaaaatacaa aagagcagac
    15361 catattgcgg tctgctctta gttttcatga aaaattattc agaaagaccc tcgatagaga
    15421 atgtaatctc aaggctgtcc tcaaatgcaa gattgtcctt gtcgatagga gaatccttag
    15481 ctgtatcgcc gtaatcgttg tagatctcga ttctgtagca gttgttgttg tcctcgatgt
    15541 tgccatactt gatcttagct ttatcgaatg caacttcctg accgtcagcc ttgatagact
    15601 taagctctac cttgagatcg tcagcgttgt acttacctgg agtttcctta tcagtcttag
    15661 gatcgcctgt gcccttgagt ttttcgccgt taagaagacc gatgatatca acgcacatta
    15721 ctacgcaacc ctgagcgcca acagcatagt cgccgtcaag atcgatctgt gggttgatgc
    15781 cctcgtctgg atcgtcataa gctatagaag cgtttgtgat agaaactgtg tactcaccgt
    15841 cgcctgttat gtcagcgtca acgccgaatg ctttagtagc ttcaggttcg ccctgacctt
    15901 cccagttgcc ccagagccac tctccgtcac cgaacataag gaatgcgtca tagcccttga
    15961 atgtctttac aggtgtgtaa tcagagtcgc tgccttttgt agtgtcagca gaactgtctg
    16021 ttgcagctga atcagcagca gactcggcag cggctgtagt tgttgttgct gcagttgtag
    16081 ttgtagtaac ggctgtagtt gttgttgcgg cagcagtaga gcttgaagaa tcagctgaat
    16141 cgccgcatga tgcgaatact actgttgtca gagaaagagc tgtgacagca gcaagaattt
    16201 ttttagcttt cattataata acctcctgaa aaataatggc agttacacta cccctaagat
    16261 agaatatctc taatgaatat gatacaaaat tttcgcattt ttttcaagat actattttaa
    16321 caaatttccc tatgataaat cgtttacatt atacaaaact tgttgaaacn nnnnnnnnnt
    16381 acagcaacat attgaacaaa tcatgcgaaa atcgtgcact taaaatatga ataacctgag
    16441 ctttatgtgg cacaatactg gcagtcagaa accaaaagga gcgagaacaa aatggatata
    16501 aatttgagca aggcacaata cagcgagatg tacaaagagg cgtcaaaggg gacaaagtgg
    16561 tgggtgacga taccaaaggc gtttttattt ggcggactta tatgcgttat tgggcaatcg
    16621 cttatggata tgtatttata tttaggttta tccaaagata acgcctcaat gtcggtatca
    16681 ataactttaa tatttttatc ggctcttctc acaggtcttg gctggtatga ccgcatagca
    16741 aagcacgcag gtgcaggtac gcttgtaccc ataacaggtt ttgcgaactc tgtggcggcg
    16801 cctgctcttg agttcaagac ggagggatat atactggggc ttggggcgaa aattttcatt
    16861 atcagcggac ctgttattct ttacggcact ttggctagtg tgctttacgg gcttatttat
    16921 tatttgtttt tgaggtaagt tgcttgcaat tcatgataac cgcacaaact cactgtaggg
    16981 gagcggcgca atttctgctt tgcaaaaata cgacgtcccg ttctgagagc gataaggttt
    17041 ggttttatca cgaatttgtg tagacgtgcc atgttggaaa atattgtgca gttcattgtg
    17101 catatatcgc atggttgagc atcaagcagg tgcgggctgt cgggggcgcc agcccctaca
    17161 catttgtgtc gatttttttc gccgcaaaat cgctggcgtt ctttgcggat tttactttat
    17221 gctatgggat agaaaaagcg gtgcagagtt tttcactccg acaccgcttt gttttttgat
    17281 ttttactttt tatacgaggg tatccttata ctttttgccc tctaccacca atattggata
    17341 gccgtcaaga tacggtttca cggcttccgg attttcgtct gcatactcaa atatctgatc
    17401 tgccagctcg ttagccgttt tcgtcagttc ctttatggac ttttcattca tagtaggaca
    17461 gaccggtgac aatgttatta ctttggtttc attcagtgcc attactctca gcggccatat
    17521 cctgcagtcg aacggtttgt catcgcctaa tatacagccc ttctcgttat ccagcattgg
    17581 gcaataatac aggtctgcat tctgttcctt atccattttg aacaagaaat ggttttcctt
    17641 ctttataaat tcctgcttag gggtatattc ctgcaatatc ttgcttgcaa gcgtttgtga
    17701 gatatatggt gtttcccata tatcatagct gtcaaagcag cagcatatcc tgcactttgc
    17761 acattcctct tttgacaata tttttgaaag cattttttct gtcatgcctg ccgtttgcag
    17821 gcatttcccc ctttcacgtc tatggtatgt tagccacact tttgtggagt gtatgttttt
    17881 actaattata acacgtttct ttgataaaat caatagtggc ttactttttt ctacataggg
    17941 agctatctgt tttatgtgag ttaacaaact atttacattt gctctgccgt attggggata
    18001 ttctctttac ggaatgtctt atttgtggca aagcctatac cgagcatcac tgccatgcct
    18061 gcccacacaa ggggttcgct tatcatgatg ccgaaatatc ccatgcgtgg ggcgagtatc
    18121 attgccactg cgaacttgcc caaaagctct attatgcttg aagctatagg tgttatctta
    18181 tctcctacgc cctgaagtgc gtttctgaaa actattacta tgccgaggat aaagtaaaat
    18241 gctgtgttta ttttcatgta tttttccact gtacccacta tctcagggtc gctgatacct
    18301 gtgataaggt gtgcgaaaaa tggtgtgaaa agccatgttg cggctattac gataacagac
    18361 cagccccatg ttatgagggt cgcttttttc acgccctctt ttactctgtc tatcctgcct
    18421 gcaccgtagt tctggcttga aaaggttgct gaagctgcac cgaaaactga tatagggagc
    18481 atatagatct cagatatttt tcttgcggtg gtgtgggcga ctattatgtc agtgccgaat
    18541 atatttattg cgccctgcat tataacagtg ccgataccaa cgagagatat cataagtccc
    18601 attgaaactc ctgttgcata catatctcct gcgagcttga agttaaagcg gaagtcgctt
    18661 tttgatggga taagaaattt gtgtttttta atgatgtata ttacacaaag cacaactgag
    18721 ataccctgtg cgataagggt ggcatatgcc gcaccctcaa cacccatttt aaagccaagg
    18781 ataaagagaa ggtcaagccc gatattagcg atagttgaga ttataaggaa gataagcggg
    18841 gaaactgtat cacccactgc acggaggata cctgcgcaga tgttatagaa cattgatatt
    18901 atcattcctg caaggattat gactatgtag gtttttgcct gcttaaagat gttgtcaggc
    18961 gtgttcaacg ctctcagcag tgggtcgata aaggcaaggc tgagggcggt gagggctatt
    19021 gccgttgcga tacctagggt aagggcgtgg gcgacggact ttttcaggcg gtcaaactcc
    19081 tttgcgccga aaaagcgtgc tgtcacaagg gcaaagccgt ttgtaagacc gttcagaaag
    19141 cctattatga cggtgttcac tgatgaagtt gcgccaaggg cggctatggc gtttttgcct
    19201 aaggtctgac ccacgatacg ggtgtcggca agattataaa aaagctgaaa aatgttgcca
    19261 agcagaaggg gcagggaaaa catcaatatc agctttgtga tatttccttt cgttaggtct
    19321 ttcataattc tttctcctat ttatgtctta tatttattcc taaaaccata ttattttagc
    19381 acaaacagag tttcaattgg taatagtact gtgaacattt attgtacata ctatacaatt
    19441 tgcggtggtt ttatttgtgt gcaataacga aaacgtttta gcaaaaaaac gcccgacaga
    19501 aatttatccg tcgggtgcta aaggggaagt ttggtgtaat gctatttaac tatgtctaca
    19561 ctttcagtgg gttttgagag atcggcatag ctgtatgagc gtatctgtct gtcggagaag
    19621 atataaatat atccgtcctg aacgaatgca ctgcggtaac gatctctctg gtcattgtcg
    19681 ttgaccatag ccgtgatctc gtcttcgcta gcctgatcga aataatcgtc aaaataatct
    19741 gtatattcgt caagctttat agttccatac tcgctgaaag ttttggtttc tatgttatat
    19801 ttcattacca gaattttttc aatagttttt tcataatagg tatattccca attgtcgccg
    19861 tcatcggtct tgcctactgt ggttatgtca tagaatggta tggctatata tccgttttcg
    19921 ttgtctgtta ttattgaatt tgaaaaaaca gtgctggcgg atctttgggt catatccttt
    19981 ccgtcaactg ttgttcccat ttctttctcg tgatatttgt attcctgtat agtcttgaag
    20041 aagttgaatt tctttactac ctctgtgtca tagacctgca cctgatccac tgtgccgtta
    20101 gcattcgcta tgcccttgcc gcttgagccg tataaaaggg tttcttgctt ttctgttttg
    20161 ctcttgtcga gtatttttac gctgaaagag tcgaatgacg tgctgtcggt gcgcacggcg
    20221 atatcatcgt tatagttcgt accataatgg aagaagttgt ttgccgttga cactgctatc
    20281 attttatcat caggggtcac ctgagttatc ttgttagggt ctgagaaatc atatacttca
    20341 tcaagctgga tattgttcgc tgcctgataa gtgtcatcat tgttgtaagc ggaagaataa
    20401 tctctgtaaa catacagcat agaatgatag aacgtgttat atcttgcact ctcgaaatca
    20461 gtgtcctcgt atgtgagggc ctttttcaca gggacgccaa ggtcattgtc aaaaagccac
    20521 agcgtgcaga tattaatgcc gctataatag gtattgtgtg tgctgatatc gtaaatgttt
    20581 gcagagtcgt ttgagaacag cgacaatctg ccgttatact catttcctga tatggcgtgg
    20641 cttatatgct cggcgtctgc gcctgtgacg gtctgggttt tgttaagctc catgtcgccg
    20701 ccaccgtagt ttattcttgt aataagcgct cttgttggca ctgtgctgta atcatcgtct
    20761 gttttttcaa agccatatgc tcttaaaata taaatgttgt ctgttcccat taagatgctg
    20821 ctttcaccgc aggcgataag ctttgaagcc atgtatgagc cttgattttt tccgtcaaag
    20881 gacgaaagca agatcatgga cggtgtatta tctctggctg tgatatacat ttcactttct
    20941 tcaaaaagct gtttttcacc attgatgtat cgcacgggtg tgatatcctc gggcttttgg
    21001 tcatctaata gcagtgaagc tgctcgggtg tagcgtgaca cgatgaaaaa ctttccgtca
    21061 acttttctta tgccctccag cacgccgggc tgttcaaact cgcttatgag cttgatattc
    21121 tcagggtcgc tcacgtcaaa cacgcacatt ccgcagtatt cgatatcgag gtttttcttt
    21181 cctgccttca cgggtaagat aaagtcgaag aaagcgtaaa gctcgttatt atccaaatac
    21241 attgcaacaa cgcttggagc gaacttacag ccgtattgtt cgtattcggc aatgtcgaca
    21301 agaccttttt taaggaaggt ttccgcaaag atatcctgtt ctgactttgt gatatttccg
    21361 ttttgggtaa tataaacgtc gataccgcca ttgtcagtaa tgaaatgggc atttccgtag
    21421 gttatgtttg tttccagctc atctatgctg gttgtggggg tgatatccac gcctgagcga
    21481 gtgagctgta tatcagcggg gttaggggtg agctggtgat ctctaagtgc ctgcttatac
    21541 aaagccacat agtcatcgtc atcaacagtc aaagtgtcga tatttttgaa tatatagcta
    21601 ttgtcaacta taaagtcctc tatgtttttg ctgttgctat ttatatagtt gtaaagaccc
    21661 ttataggtcg ttccacggag gccgtcaaga cctgaggcgt cagcggactg tgaagaagcg
    21721 gaattggggt attttttgac gaactgggta ttgctgaacc taagcacagc aaatccgcct
    21781 accacaagga cgaaagccgc caccacagcc agacatctca gggcgttgct tctgccgctg
    21841 tttattttta tctctgcact atttggagta ttttcgataa cgggtctgaa gcctgctacg
    21901 tccagcatat cttttatttc ctggggcatg agcttatcag gaattttgtt tgcctgttcc
    21961 atttgtctga taagctttct gcgttttctt aaatttctca tcatgtcacc tcctttccaa
    22021 acagcttact gcgtattttt gctttacctc tggatatatg ggaacggact gtcgtatctt
    22081 taatacctgt tatctccatt atctccctgc ctttgtagcc tgctatacag tgaagtgata
    22141 tacagagtct ttccgcttca ctgagatcag caaactcttc taagatctcg caccgctcga
    22201 atccgttttc gtccttgatt attgcgtcgc tttcattaat ataatcgagg ttagagcgtc
    22261 tttgggcgta ttccttttgt ttttgtttga tcttggcgac cagaattttg aacatccagc
    22321 tatcgaaagc ttcgcttttc cgcagcgagg agatgctcat atagccgtcc agcacagcgt
    22381 cctgcactgc atcggcggcg tcctcggcat tttgcaggtt agcgagggcg taataataaa
    22441 gctccttata tatctctcca tagagcttag aaaaggcgtc cgtatcgccg ttcaccgcaa
    22501 gttctgcgag cttttttctt ccctttttgt ccaatctgat cccccctgtc gtccgtcata
    22561 atataagtgt atatttttca ctgaaatgtt gcagtgaaaa aaattttttg aataaaaaaa
    22621 tcggggacga ggtggtcgtc cctgatgata tttgtttatc aaactgtgta ggtgctgagg
    22681 gggtaacttt ggtctgctat gctaattcct cattcctcac tcctcattcc tcatttttca
    22741 caccgcatac ccattcctgt tcaacaattt tatgaccttg tcataattaa gtccctttac
    22801 agtatgatag tctgtactct ccgaattggt ataaactctc acggcacaag tatggcttat
    22861 cttctgaaac ggattttgca tgaccgttac cattgacact ctgtcaagtg cggctattga
    22921 tttatgaaag ctataccatt tacaatatga caatgtacaa tacccgtttt caaagcctat
    22981 tgatgttgtg aacgctgccg ccgccttgac tatcacgagc cacagcaatg gtatggtagt
    23041 tagtataaca agtatggtta tttctctttt ccagttagga aaaaagtaaa gcgctcctct
    23101 tcctgatatg atgggtataa acgtgcatat gagcggtatc gttataaatc tgccaaggtc
    23161 ggcttttcct gtggagatgt cgcttttcac cggcggtatt ttcggcatga gcatcttcag
    23221 ggagcggtca acctgtccgt ttgtcgttat cggtataagt gctgatattt caagccgcct
    23281 ttttccgtat cctgtacagt gggcagacac agaggttatt ttgcatatct tcataagcaa
    23341 tgattgtctt aggtcgatat agtttactct atctctatag aggacgtgtc tgcgtcttgt
    23401 gcctatcccg cttctgatta tgaaaaggtc ggcacagcgt gtgcaggaaa aattccagtg
    23461 gcgcataaga ttagacagaa atgatatgag ccagcctcct gccagcacac cgcctgcgat
    23521 aaggatatat tcaggcactt ctgcaagcac cggaactttt gctatctccg tattcactct
    23581 gcggagtatt tgttcctccg cctctcgtcc tactatccta tatgcttcgt aaagtaccga
    23641 cagcacgatt atcatgcctg acagagttga tgaaaagata agagagaata taacgagcag
    23701 gcttttcttt gagttgtaga catatttagg tttgtttttg catttggcag tggcaaaatg
    23761 ataaagctca aaggctcgtt tttttgtaag ggtgagcatt atatctgcat tttgcagtga
    23821 ttttgcatcg gtgtctatgt atacggcaca agaatgtata agtctggaaa tatatccttg
    23881 tgacagtgac aatgagctta tctcggcaaa atagaccttt gttcgtgtta tgccgaaata
    23941 gcctgtgtgg gctatgatgc aatcttcttc aagctcatag tagacaaata tccagcgcag
    24001 gaaagcgtag ccgaagataa atgtgatagt gaggatatca acccaatgtg cctgaaacca
    24061 gctttgaaag ttgaattttg cggctatgag atacttggca agagggatag caagaagcca
    24121 catatattta gttgtataag ataatatcat tatagggtgc tggcggtggg tgaagaaatt
    24181 cttcagccag cttctttttt ccttgatagt ttctctgcgg ctcatttaaa cctttcctgt
    24241 ctatggtatc tagtcttctt tgcgttctct gtttcgtatg gagctgttta ctatggcgga
    24301 tatttccatg gcatcacggc ggctcataaa aggtataaga agtcttgcgc ccaaggcgtt
    24361 taagacaacg aagttaagtc ctgttatact gcctagggga gttattatgg tggtgacgga
    24421 ctttattgcc gaggtgggca taaaatgccg catggtaaag aaaagccctg taaaggcggt
    24481 gacttctttt gagctgacgg tgaaataaga tctgatgaaa taaaatggaa gcaccaacag
    24541 gcaaaaaagt gcggcgatta tccacattgg cataaggata taattcacgg aaaatgggat
    24601 aagtttttct atgagattga ccgcatagcg gataagaaac aatattaccg caaggatagc
    24661 ccagaagata aaaagcggta tttttgaggg cttatatctg tgcatttgag gacgttcacc
    24721 tcgctgtttg tttttttgcg tatttgtatg tatggttatc tcagttttca agaattaatc
    24781 tcccttttat atcatatcat ttataaggca attatttgca cgaaaaaagt gttttcgggg
    24841 gaaagctatg gaacttacgg ctataataaa caagataaat tcgtttttgt ggggcgttcc
    24901 gcttttgata cttttgcttg ggggcggaat atatctgagc ataaagcttg gatttccaca
    24961 gatacac
//
LOCUS       DAWEMR010000012        24378 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_845_length_24378_cov_13.046499, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000012 DAWEMR010000000
VERSION     DAWEMR010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 24378)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24378)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24378
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_845_length_24378_cov_13.046499"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3662..3674
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21630..21639
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6755 a         5776 c         4998 g         6826 t
ORIGIN      
        1 cgttgtaagc ctttccaacg cccacgttct cgtctatctg aacgatatgg aaaatatgct
       61 tgaactcgtc ctgaaatgac tttacgataa tattaagctc gcaggtgagc ttgccgtcgc
      121 acacaagcac gaagtcagag ggaggatatg actgcatgag catagactca agactctcgt
      181 taaggttctc aggaagctca cggtcataca ccgacataag cacactgtat tcaggcagcg
      241 tatattttcc aacacgatac gtcataaaat ccctcccctg cattattatc aactcaatta
      301 tactataaca gctccaaaaa gtcaactacg accaacaaag tatactattt atatagtaca
      361 gaccaataca caaaaaagac cgccccattc ggaacggtct tgtaagctac tttgaaaaat
      421 tagccaagct tcttagcaag ctgtgatacc tttcttgcag cagcattctt atgcataatg
      481 ccctttgtgc aagccatatc taccttcttg atagcagctc ttactacttc ttccttgttt
      541 tctgcattct cagctacagc agcgtcagcc ttcttgagtg cagttttaag atcagacttt
      601 acagccttgt ttctcattgt cttagtgttg ataaccttga ctctcttctt agcggattta
      661 atgtttggca taccgacacc tccctcattc tgtataaatt tgcacttatt tgcacaagct
      721 atccgcagac tgagaggaaa tgcggaaagc cctttaaaca agccttgtaa aatattttac
      781 cacatttcaa aagaaattgc aagggggttt actgaaaaaa atatagaaat tttgcggcct
      841 aaattgtagg aacacacaaa aacttacacg atatcacagg aaggtgaata aaatgagcat
      901 tagaacggat atggcggcgg agagcttttc tcaggcaggc ggtgcggaaa agcttcaggg
      961 agtgaaagtc aaaaacaagg ttattgaaaa cgctaggctt gaggtaacgg acatcaccat
     1021 agaaacacag gaagccgccg aaaagcttgg cagacctgtc ggcagataca tcacccttac
     1081 cgccacagat tcaacattcg atatgtacag cgacagtttt cgtgagcgtg cagaagtcat
     1141 agcaaaagcc ttaggtgagc tttgcggtgg atgtgaacgt tttttggcag tagggcttgg
     1201 gaactatgcc ctgacttctg acgctgttgg ccctcttacc gctgaaaaga tcttcgctac
     1261 ccgtcacata aaaagccttg caaaagagat tgatactgac gatctgggcg aggtcacagt
     1321 gatacagcca ggagtgcttg gaaacacggg aatagaatcc tcagagcagg taaaagctat
     1381 cgcagagcgt gtaacaccgc aggctatcat tgccgttgac gcactggctt gcagcgagct
     1441 ttcaaatctt ggcaggacaa tacagctttg caacaccggc atttctccgg gaagcggagt
     1501 cgaaaacgcg cgaaaagagc tttcgctttc gaccctcggg gtaaagtgca ttgccatagg
     1561 tgtgccaacg gtcatagacc tttgcactgc cgcacagcac attttcggtc agacagctcc
     1621 tgaaagctcc gaaaacatca tggtcgctcc gaaaactgct gacaaacttt cggaaaactg
     1681 cgcaaagctc attgcaatgg gcataaaccg cgccgttcac cctgccctgt cgcaagagga
     1741 cattcaagct ctcacctgct gacaaaaggc aggtggtaca gtaatgtaaa acctgtatac
     1801 aacaattgta aactttttat taatatgtat gagtgaaatt gcgaaaacgt tttctgaaaa
     1861 cgaatactca cacaaattca acacaccttt gtcataatca acaaaaacgc ttgagcttta
     1921 tattgcaaaa tgctgattga ctaaaattaa actttttgtt atcattagta aggacgaaaa
     1981 ataagcgtta tggcaagcgt tgtcgggtaa caatgcttgt cgatcgtaac agatgaacgt
     2041 tttttgggta aggaacattc gtctattatc aaggaggtta ctataatgaa aatggcaaag
     2101 agaattttcg ctgctgcttg tgcgctttca ctggcttgca gcatggctgc ttgcggcagc
     2161 tcatcaagtg acagctcatc ttccaagaag gaagctaagg ctatgacaga tgatcagaag
     2221 gagcaggtaa ataaacttca ggataagctc cctgacatcg agctttcaaa caagactatc
     2281 aagtggatgg ctcactatga catcaaccct agtgatggta agtcagaatc acctgctatc
     2341 tcacttttcc agactaagta tggcggaaag atcgagtacg ttcagacaac ttgggataac
     2401 agatatactg atcttgctaa ggcagttatg gctaacgact cacctgactt cttccctgct
     2461 gacgacatgg acgcattccc taagggtgct atcaaggcca tgttcgagcc tatcgacgat
     2521 gtagttgatt tcagctcaga catctggtca tcatcacaga cacttaacga ttcattcgta
     2581 ttcaacggca agcactatgt agctgctatc gacgtaagac cacagtatgt ttgtacatac
     2641 aacacaaaga caatatcaga ctttggctat gacgatcctg ctgagcttta cgctaatgac
     2701 gagtggacat ggagcaagtt cacagagatg tgccttgact ttgcagactc tgaagctgac
     2761 agatatgctc ttgatggtta ctggtacggc aaggctctta acgatacttg cggttatcca
     2821 cttatcggtc ttgaggacgg caagcttgta aacaacatgg gcaaggctga agttggcaca
     2881 gttcaggatc ttatgtacaa ccttgagaag aacaacgttg tattcgacag atcaagcaac
     2941 aactggaaga caagaggtga cggagctaac ggtgaaggtc ttggttcaca gctcacactg
     3001 ttcattccta tcggcacatg ggctctcgag gatactcctg aaaagactaa gacattcgga
     3061 agcgtcaagg acggcgaggt tatgttcgtt ccaatgccaa gaatggacga ttctgacaag
     3121 tactacgtat ctacaggcgt aaacggctat ctcctctgca agaatgctcc aaatccagac
     3181 ggcttcaatg cgtttgtaaa ctgctgcaag gttgcaaatt ctgaggtaca gaacattaca
     3241 gaagagcagc tcaagaacga ctacggctgg acagacgata tgatcgagat gcgtaagaca
     3301 atttacgatc tggctagaca gaatcctgta tttgacttcc aggacggcgt atctgctgag
     3361 ctttcaacac ttatgcagac agtaaaccag gctacaatga taacaggcgg cggagctact
     3421 acatggacag agtgcgttgc tgagaaccag aaggctgttg attacatcat taaggaagca
     3481 aacgctaacg ttgctgaata attcagtatt ttcaagcggg acgttcattc gtcccgcttt
     3541 tttattgcct ttgtatttgt cacgaatttg ttaaatatat ttgtacgcaa aatgcacaat
     3601 ataaaataca taaatttgtg caagaatttt acggattttt atgtacaaat cacaaaaaat
     3661 annnnnnnnn nnnntgctat aataaataca cgttaaaaag aatatatcaa agaagggaga
     3721 tatgttttat gagatcgatt attactatca gccgtgaatt tggcagcgga ggacgtgaaa
     3781 taggcaagaa gcttgctgat gatctgggca tacctttcta tgataaagag cttttggaaa
     3841 tggcttcaaa agagagcggt atatgtcagg agctttttgt taagaacgat gagtcctata
     3901 ctaacagctt tcttttctca ctggtcatgg gtaattaccc tgtaagtgca gacggcagga
     3961 tcaatcctga tatgccgctg aatcacaaga tattcttggc tcagtttgaa actatcaaga
     4021 acattgcaaa gaagggtcct tgtgttattg taggacgttg tgctgactat gttctgaaaa
     4081 atgagcctaa tattatcgac ttcttcattt ccggcaacac tgccgaaaag aaaaagcgta
     4141 tccttgagag atacgatatc gaaaaaaaca aagctgagga ctttatccgc aagactgata
     4201 agcggagagc ttcatactat aattattaca ccgacatgaa gtggggcgaa gcaaagaact
     4261 atgacctttg tatcaactcg tccaaaacag gcgttgacgg tgctatcaag cttatgaagg
     4321 cttatatcga aataaaagaa gccaacgctg ataaatagga atttttcgtt ttaattcatt
     4381 ttactttcct tactacgaaa gagggctgac catagggtcg gtccttttcc gttttatgag
     4441 aagttttatt actccagctt aaacaaagtg taaccaatat gttattgaca ataacaagca
     4501 aatatattat actgtataaa agggcagaag tccgacaaaa aaataaaaaa atactgcaaa
     4561 aggagaaaca gaaaatggct gaaataaaag ttatcactat cggcagacat tattgcgcag
     4621 gcggttcgac tatcggtaag cttgtggctg aaaagcttgg tatcccatgc tatgacagag
     4681 agataatcga catggcggca caaaaaagcg gcattgcaat gagcgagata caaaaatacg
     4741 aggaagccgc tcttagcatt ctcaaaacac cgataaatct gttttcaaag gagaaagagt
     4801 atcctataac agagaagata ttcgccgctg aaacagaggt aataatggag cttgtttcaa
     4861 atggaaactg cgttatcgtg ggcagatgtg cagattttat actgaaaaac aaagtcaaga
     4921 cgctgaatgt cttcattaat gcaagctttg agaagcgtca agagtgcgca attgagaagc
     4981 acaacatctc cgaggacgag gtagaagcaa ggctcaaaaa gtatgacaaa aaacgtgccg
     5041 actattacaa ctccaacacc aacaaaaagt ggggagatat ttcaagctat gatatgtgcc
     5101 ttgacagcgg caggcttggc tacgaaatgt gcgccgagat aattgctgac actgtgaagc
     5161 tttcaagggg gaaatagggg caatatttta ttgggagcga taaaaaatgt caaaggataa
     5221 ggaagtcaag accaacgcca tgcgtattct tgacagaaat aaggtaaaat acaaggtcaa
     5281 cacatacgag tgcgacgagt ttatcgacgg catacacatt gcagatatgc tcggtcagcc
     5341 atatgaaagc tcgttcaaga cccttgtgac agtgggtaaa agcggaaatt actatgtttt
     5401 cgctatccct atcgcagagg agcttgacat gaaaaaggcg gcaaagtctg tgggcgaaaa
     5461 gtcggtggag atgctccatg tgaaggatat aaactctgtt actggctaca ttcgtggtgg
     5521 ctgtacaccc cttggcatga aaaaacagta catgacggta gttgacagct ctgcggagca
     5581 gtttgaggag ataatcataa gcggcggacg tatcgggtcg cagataatgc tttctccgaa
     5641 agaccttatc aaggtcacaa ggggtaaatt tgagagcata acaatgagtg aatagacaaa
     5701 aaaagagagc cgcttggctc tcttttattt tgcatttatg ttgatatatt cgggctttgt
     5761 ctttgagtac attattttct ggctggaata aagtccgtaa tagcccgaaa gcatatagct
     5821 tactccgcag gcaaccgcaa aaagcacaag cccacttcca ccgaaaagct catagctgag
     5881 aagtatggaa gccacagggc agttcacaac gccacagaaa agtgccacaa gacctatagc
     5941 cgcaccgaat gatgggtcaa gccccaaaaa gcctgcggct gtacagccga aagttgctcc
     6001 gataaagaag gtcgggacta tctcaccgcc cttgaagcct gtatttatag ttatggcggt
     6061 gaaaagcatt ttcagggcaa aggcttctgg gtgcgcctta cctttctcta aagccgccac
     6121 gataacgtct acgcctgctc cgttataatc ccttgtacct acaagcaaag tcaatgcaat
     6181 gatcacaacg ccacctacag ctatcctgag ataggtgtta ggcagataca tctgaaatct
     6241 tgaatgtgaa aactccatta ccatgcaaag gagtatggat atcaccgcac aaagagccgc
     6301 aagcgccgct atctgggtca cagacacaag ggaaagcctt ggtatgcctg ttatgttgaa
     6361 ctttgttggc gcaagccctg ccaacagcga tattttatat gcgataagag aggaagctat
     6421 gcaaggcaca agacccacat agtatataac gcccacgctt attacttcca tagcgaaaaa
     6481 agtcgcagtc aaaggcgttc cgaaaagtgc ggcaaacact gcgctcattc cgcacattat
     6541 cacaaggctg gagtcttttt catcaagctt taaaagctcg cctgactttg cgccaatgct
     6601 tccgccaagt tgcaaagccg caccctcacg tcctgccgaa ccgcccgtaa ggtgggtgag
     6661 tacagtgcct agaaagatga gaggcgccat aagcagcggc actttgatgt ttccacgaac
     6721 agagcttatg ataagatttg taccggtctc ctcgtccacc ttgcagacac gatagctgaa
     6781 aatgataaac agacctgcca acggcataag atacaatgtc cagctatgac actcacggaa
     6841 gcctgtggca aagtttaccg ccatatggaa cagcgagccg actactcccc ctattacacc
     6901 cactgcgcag gcgataaaga cccactttgc aaaggctatg gagaattttt tcaccgtccg
     6961 cattttgctt tttataaact cactgcttat tttcctcacc cctttttaca gaaaaacaac
     7021 gggcgacata tgccgcccga aatatttgaa atacagctcc acaataatgg gacgtcgcat
     7081 ttctgtgaaa cagaaattca gccgttccac tacaagataa tatgctaaat tatacctcag
     7141 cgattacctc tacctcaacg agaacgtcct ttggaagctc ttttgcggca acgcatgaac
     7201 gtgcaggctt tgatgtgaaa tactcgccgt aaactccgtt aaatgcaccg aaatctgaca
     7261 tattcttaag gaagcatact gtctttacag ccttgccgat agatgttcct gcggcttcaa
     7321 gaacgtttgt gaggttcttg caaacctgat gtgtctgctc ctcgatagtt gttgcctcaa
     7381 cgttgcctgt tgctggattt attgctatct gacctgatgt gaaaacaagg tttcctacct
     7441 tcattgcctg tgaatatgga ccgattgcgt ccggtgcgtt ctttgtgtaa acaacttctg
     7501 ccatttttat ctactcctgt cttaaaatta cttattgact atcttaaagc ctgcggctgt
     7561 aagagcctgt cttatctctc tgatatgatc gaagtttctt gtttcaagaa caagtctgag
     7621 caagcaatct gtgatatcgc tgtcctcgct tgctctctcg tggtgaacag aaacgacgtt
     7681 tccgccaagc tttgaaatta tctctgaaac atctctaagc tgaccaggtc tgtcctcaag
     7741 ctggatagta agagtatcag atctgccaga cttgagcaga cctctcttga taactcttga
     7801 aagtatagtt acgtctatat ttccgcctga aacaagacat acaacctttt tgcccttgat
     7861 gtcaggtatc ttattaaaca ttactgcggc aacaggtgtt gcgcctgcgc cctctgaaac
     7921 gagcttttgc ttctcgataa ggtggaggat agctgatgat atctcgtcgt ctgaaactgt
     7981 aacaacgccg tcaacatact tgctgcaata ctcaaaggta tgctcgccag gctcttttac
     8041 cttgattccg tctgcgatag tgtgaacgct ttcaaggcac tctatcttgc cgtctttaag
     8101 tgaatttacc attgaaggtg cgccttcagc ctgaacgccg tatactctaa cgcttggctt
     8161 gagctgtttt acagtgaatg caacacctga gataagtccg ccgccgccaa ccggaacaac
     8221 gataacgtct gcgtcagcca cctgacttag tatctccaga ccgatagttc cctgacctgc
     8281 gataacgttc tcgtcatcga acgggtgaat aaatgtatag ccgttagcgt cacgaagctc
     8341 gatagccttt gcataagcgt catcataaac gccaggcaca agacatacct cagcgccata
     8401 gcccttggtt gcctctacct ttgagatagg tgcgccgtca ggaagacaga tgatagactt
     8461 tataccgttc tttgttgcgg cgagagcaac gccctgtgcg tggtttcctg ctgagcaggc
     8521 gataacgccg tgtgactttt cctcgtctga aagctgtgat atcttataat aagctcctct
     8581 taccttgaaa gaacctgtta cctgaagatt ttctgttttc agataaacgt cacaatcagg
     8641 gtttaccttt ggcgcatgga tacacgctgt tggtctgatg acctctttaa gcacgtttga
     8701 agcgtcaaaa accttatcca atgtaagcat aaaaaacaac ctcttttttc tatgtaataa
     8761 aaaaagccct cgcctgacta gaggcgaaga cataatatct ccgtgttacc actctgattg
     8821 cagaccataa gcctgccact catatctctt tctaacggtg agaaaccgta tccgcttaac
     8881 acatctgctt aggcggatca gctcagagat gatcctgacg gcgttatccc tgcggacttt
     8941 caccaaacgt ccgctctctg cgaagtattc gcaccgtcac ctttctcgtc acagccttta
     9001 cgatattcaa tacaatcatt atatcaccat aaatatgaat tgtcaacagc aataaaatga
     9061 atttttctct aagacattat acaaaaaata aaaacccgca cgagcgtgca ggtttttatt
     9121 cattaaggaa gtatagaaaa aatatatgaa gaaaacaatc atgaagtctt tttaactgtc
     9181 ggtctttcct ttccgactgt aataatatta ccactgcaat gtgatagaat tatgtgcaaa
     9241 gagagtgcat ttgcaaaaat atcacctttt gtttgcgtaa tgcacaacga tatgacgaag
     9301 cttccctgtg gtcatatccc tgccctttat atccgctccg ttgtggctca taagctcagc
     9361 gcagactatg ccgtcggtac tgcacctgaa aacctcgtgt ccgtcacaca ccaccacaag
     9421 cttttcacta tcaacagata ttccgcctgt tcttccgata acattttcct ctgcgccctc
     9481 acgctccacc acataggaaa ctcgtttccc atgatagcgt tcaagctctt tcaagggcgt
     9541 acctttattt tttctgaaaa gtcccatttt acgctcctgt atcaattatc tgctatatta
     9601 ttatcatttc ctgcacaaag ctgccttgcg gagacgataa cgtcaagcga ctcgcccacc
     9661 gactgcaaaa aggctgacag caacccaagc tggtcaacgc tcaaaccctc gccaagataa
     9721 tttgcaaatg ccgcagccat tatattaagc tgctgaccgc agcacgatcc ccggttttcc
     9781 actgataccg ccctttcctg ctcacaacgt ttctctgtca taatatgcgt cagaagaata
     9841 atacgttcgc atatgcagat aataagggca tattctgaaa ggggtcaaac acctatgaac
     9901 atcactcaca ccatacttgc cacagtcggc tctataatcg ttatgtttgc catatcaaag
     9961 cttcttggca acaagcagat atcacagctc acgctttttg actacatcaa cggcatcacc
    10021 ataggctcta tcgcctctga aatggctgtt tcgcaggaaa tggacgagct tatagtgcct
    10081 gttatcgcaa tggtgcttta cggactgaca ggctttctgt tgtcggtgct tacgatgaag
    10141 tctatccact gccgacattt tctttcgggc aagcctatcc tgcttattga aaaaggcaaa
    10201 atatatcccg aaaaccttaa gaccgccaag cttgatgtga atgatctgct ttcaagagca
    10261 agaacgtcag gctatttcga catctcaaag atatactacg ctattctgga aaccaacggc
    10321 actatctctt tcatggaacg tgccacagaa gcacctctca ctccaaaaga cttaggatat
    10381 gagaagcatg acgaaaagct ttgcaccgac cttatccttg acggcgttat tatggaggac
    10441 aacctgaaat acgcaggcaa ggagataaaa tggctcacca acgaaataag aaagcaaggc
    10501 tgttcatcac ctgacaaggt ctttctctgc atagttgacg gaaacgacaa ggtgacagtg
    10561 ttcccgaaaa ctcaggagag caacaagaag atctacttcg actgaacctt cttcaagaac
    10621 tcgtgcagct cctctgcaag cctgctcaca ggcagaccaa caacgttata aaagtccccg
    10681 tctatctttt ccacaagaag tgctccaagc ccttgtatgc cgtaagcccc cgccttatcc
    10741 ataggctcgc cgcttttcac ataggctttt atctcctggt cggaaagctc tctgaaagtc
    10801 actgcggtat caacggaaaa gctactgtaa cgacagcctc tgccaagggt aacgcctgtt
    10861 acaacatgat gagtcttgcc tgacagcatt tttaacattc ttatggcatc agcttcgtcc
    10921 acaggcttac caagaacttc cccatcgcat attacaacag tgtcacagcc gataacaaca
    10981 ctcaggtcat agaccgaagc gatacatctg cattttatcc ctgaaagata ctcaggggca
    11041 agctccggtg taacgtcctc cggtattttt tcgtcacaat cagaggggat aaccctgaaa
    11101 ttctcacaca tcaatgacag cagttctttt cttcttggcg acgctgaagc aagtataaca
    11161 tcataatcca taagctcgct tgaaatattc actgacataa agatcttcct ttacttcttt
    11221 tacttcttac tcaaaaaggc aacgcacttg ttatcctgcg ctgccttagt ttttttatta
    11281 caacttcatt ccgcactctg agcagaactt aacgcctgca ggctctttgt ggccgcactg
    11341 agagcagaat acaaaactgc tgtttgcagg ctcttgcacc ggagcttctg ctatcaccgg
    11401 agcaggctgc ggcattggtg caggctgagg tgctgtttca ggctgtggga taggtgcagc
    11461 agtctgtgcc acagcaggct cagggatagg agcggctgtc tgaacattct gctgagcagg
    11521 attgccataa acaggattag cctgtggctg agtataagca ggtgaaacag ggtgattatg
    11581 atagccctga ctgttttgta taacaggctg tgcgcccatt ggaaccgcac cgccctgagc
    11641 aggctgctga taaacaggct gagccatttc aggctgtttt gttccgcagt tagggcagaa
    11701 cgccatattc actggtatct cgtggccaca gccggaacaa agtttcattc ctctgatggt
    11761 aagaagctgt tcattaagat ttttcagttc ttcaagagtt gcgtctatct gagcaataag
    11821 gtttttcacc tccggctctt ctatcttgtc agccattctg agcttgacat atctgcctat
    11881 ctcggtatac ttatcgttta taaaggcaag ctttgcgtct atctgtgcct ttactgcatt
    11941 ttcaggtgta ttcttaggtg aaaaaaatcc catttttatt ctccctttcg gttataatat
    12001 ttcttctgta ttttgctcta tggcgatatt atcctcaggc tgatcgtcaa acgaagttgt
    12061 tgtagaatca ggtgcttctg tagcaggagc ggtggtaaca gtgccgttct cggtcggctg
    12121 agtctgtgca ggctcactgt cctctttgct gtcgcctgaa gcgtttccgc cctcgtcaac
    12181 tatctctgaa tcctttatct ccttatctat ctcgtcctgc gacatctgac ctctctttac
    12241 agctttgtca tttacagccg acagaccaag atagcaattc acataatagt gagtaagtat
    12301 ctgatcatat gtatagccag cctcggcata atagcaagcg ccccactgcg acattccaac
    12361 accgtgaccc cagccatagg acttgaaagt gaacacaccg tccttatagc ttatctccat
    12421 ggcgttcgat ttcacatcaa agaggttgca gagggtgata cctgttatcc tagcttcact
    12481 gccatcatag gtacagctcg tatggccgtc gatagtcact ccgcttatat actttccgct
    12541 gtaagcagag tctattttaa accagttctt cacgtcgtcg gaaagcttta tatcagtttg
    12601 gctctcgata cgctccttga cctcgtcttt tgtatatgta gcttcaatgc cccagttagg
    12661 atctttatcg tcaaactcgc tctttacacg cttgagatac gggtaagaaa caccccatat
    12721 atcctcgctg gtggtagaat aacctgctgt tgaagcagaa taaactgcat ttatgatgtt
    12781 gccgttatac ataacggttt gacccgcaac ggcatttaca catctttcta tctttgaaga
    12841 ataaccgctt ttaagtccca ccataggtat agcaccgata gaatcgttaa atctgaccca
    12901 tgtataagcc gccacggcct gcgcctttat ggcttcctcg ccccattcac cgcctatctc
    12961 gctgttgact atctgacaga gcagttcatg accattaaga gtgacgatat ttcctgagat
    13021 tatatcgttg acagtataat actcatcagc gacgcttctt gtatctgtaa agttagcagg
    13081 gtcgttcttt atatcgtaat actcgccctt atcctcaggc atttccacag taaactcact
    13141 ggctgaagcc tttttatttg tggtctttgc ctgctttttc tgctgaacat aagacatatc
    13201 atacttatcc acagaggact ttttgaactt cacaaagtca tagacctcag tagcggcagt
    13261 ggtcttaacg ctttgagctt caacgttttt gacctgcacc gcaggggctt tttctgcgcc
    13321 cacgctttta ttatccagca gcagttcacc gcatatacca acggcagtta tgctcattac
    13381 tgctgcacag acatttgcac atatcttttt tgtcatcaaa ttcatcgtca agacactgtc
    13441 cttaccttca agcggtcaca ccgcaaaaca ttcccaatgg cagcaaaata ctcactgcat
    13501 acctaaataa tagtttatca aatactgaaa aaaagtcaag acaaagctaa gtttcgtcct
    13561 gacttttcag cataatatac aattttacat ctacaatatc gacattttag ccaaaaactt
    13621 caaatattac ttgaagtatc tctgaccatc aagctccatg cagaacgtga gtgattcaaa
    13681 ccaagccgct gtgcttccac cgcaatactg tggagcgtag taataaactg caccgtttga
    13741 attatcctcg ccgttcagcg ctctctttgc acaatcaata gtgttctgag ttggcttgtt
    13801 tctgccactg taatagccgt ttattgcagt gaactgattt ggcgctgtaa gaacgtcgct
    13861 tatagagctt ccaaatctgc ctgtcttcac tctattgata ataacatttg caacagcgat
    13921 cttgctctgc tctgagcagt ttcctacctc gccctgaaga acgtagcaaa gcatatcaaa
    13981 ttcttcatct gtatagctta taacagaact gttcgagctt gatgttgttg ctttctttgt
    14041 ttctgtcttt tttgtttcag actttgttgt cttagccttt gttgtcgtag tcttttttgt
    14101 ttcagacttc ttagctgctg ttgtagtttc ggacttcgca gcggaagttg ttgttacttc
    14161 tgccttttta agatagtcct tcattacata tccgctgtag tcgccgtatg taaccttata
    14221 ccaaccgtcg cttgtttcag ccgtaacgat aacttcctcg tctgttgcaa cgatagcttc
    14281 tatctctgag ttcttgttag ccttttctct gacgttcacg atctcagttg cgaccattgt
    14341 tacgttattg acctttgttt cttttgctgc atttttagta gtttctgtct tttttgttgt
    14401 agtggctttg gcggaagttg ttgtctcaga ctctgcctgt gccattttat actcgctgat
    14461 acccgctttt ctccatgtaa cggtgttcat tgaagtatag gtctcatctc tctcagtttc
    14521 tgcttctgtc acagaagtgg tcttagtgtt ggttattgca gcttttgtgt ccttctgagc
    14581 ctcgttgttg ccgcttgtaa gtgcaaagat acttactgta cctatcatca gcactgccag
    14641 cgcaccgctg cccagcattg ttcctacttt cctgaccgac ggcttagctg ttcgctttat
    14701 gctgtcttca gtttttttct gcttcatttt aatcacatta ccctttccga ccgtctgcac
    14761 gcaaaccgtc ttcactgccg tccgtgagct tgacgtcagc taaaatttat tttacaccga
    14821 tttggtgtaa taaacatcaa taatcccagc cgaaatactt cttattgttt ccgtatacgt
    14881 ttctccagca tttagggaaa taaatatcct tatttgcctt gtccttatag gaatctatta
    14941 taagatccag gttatcgtca gccgtcattt tctttgccac gatcacccac ctgagatttg
    15001 aagtttcgtt tgtacaagtt gagagcgata tatacttatc gtctgctgta cactcgatat
    15061 cgtttgaata ccacgaacgc tttctcactt ctgagatcca atcgtcaaac ttatagtcag
    15121 cgtcgtcaaa atatctggta gtccagtatt tgaagagctt tccgtcgtcc tgattttcaa
    15181 gtattcccgt cacgaacgca cccacgataa catacttaca gttgctgtca tagatagtat
    15241 tgaactcgat aacaggatat ttcttaagaa aatcaactcc ctttttatac tcagtaagat
    15301 gagtaaaaga agttcccaca tatctcatgt tatgaccata tatcgtgata ttgtcagact
    15361 gcccgtcctt atttatggta gaccaagcgt ctgcataaat gcttcccgat tcgtaatact
    15421 gcttgtctat attcttgtaa agataatatt cattatcctt gcctttaaga acagggaagt
    15481 ttatatattc atcacctgcg gaatttttaa agccggggat ctttatccag cctacgacgt
    15541 ccttgttcct tttcagaagc ttcttagccc aaccctgaac ttctctgtcc ttcgttgaag
    15601 cctccgggtc agaaacgtca atatcgtctg agccgttatt cttgctcagg tcaacgtcat
    15661 cgctaaagtc aggctcgtaa tttacgatct cctgcatata gttctgatcc tgctcgtcgg
    15721 ctttcagaaa atcagaaagc tcattaagag aaacacagaa aagcacgata gagaacatga
    15781 acactatctt tctgaacact tctcctgctc cgtcgccctt ccacgggata aaatatctta
    15841 ctactcttac aaaaaagttt gtatcgtcct tttcggacag attcattgtt tcattagcca
    15901 ttgacattta tttcaagcct ccgccttttg ttcatacagc tcaagcacca ttctcagtgc
    15961 tgtttctgtt gtaagcatac ggatctttct tctgtccaaa ttctcatatt ctttataaag
    16021 ctcaagccgt ctgactattt ctttctgacg acttctcact gaaacataca cagttcccac
    16081 aggcttttcc tgagagccgc cgcttggtcc tgctatacca gtaacagcca ctgcacaatc
    16141 tgcaccgaaa agcttcagcg cacccttgct catctcactg gctgtctgct cagaataaac
    16201 agtatactgc tccagcgttt cctttttcac gttcagaagc ttcatcttta tttcctccgt
    16261 ataggtgcaa gcacctccga ggaacatttc cgacgctccc gaaacggaag tgatcgtctg
    16321 gcttatcatt ccgcctgtac agctttccgc agtacacagc ttccgaccgc tctttatcat
    16381 atattttact acactttttg taactatgtc aatactatct gtaaccactt tgtcaccttc
    16441 agcaaatttc atagtaataa tcaccccttt tagttatata ttgcacataa aaaatcggct
    16501 ctttttgtcc attttgttaa ttcgacgatt ttcaaaaacc aatgttaata gcaaattctg
    16561 caagcagatg ttacactttt gttaccaaat tactgatttt tcattattag caagcccgtt
    16621 caacaccctt ttggacgcaa agaaaagccc tgaaaattcg gggcttttcg gcatttttca
    16681 ccatggaaag acacaatatt cgggtacaaa aaagcgtaca attaagctac aagctgttac
    16741 agttttaacc cgtttgtaac cttttcacag acaccatgca acgggtgtac gccatgaaaa
    16801 tacgctatgc gtttttgata agataaggct ttgttccctc gatagctttc tggatataag
    16861 gctcccaatc gaaagcgttc tgagcggctt caacgtctgc aagatttggt cttgtcttag
    16921 ggtgctttgg cgtgaaaact gtacagcaat cctcataagg gaggatagag gtatcaaaag
    16981 tatctatctt tcttgctatc tccactatct cagatttgtc cataccaacg catggtctga
    17041 atacaggcat acggcaagcg gcgtctgtgc agaccattgc atacatagtc tggctcgcta
    17101 cctgaccaag gctctcacct gtgacaaggg cttggatatc ctctttctcg catattctct
    17161 gtgctatctc catcataagt cttctcatca ggatagtgaa aagctcctca gggcagttat
    17221 ctctcagaag ctcctggatc tccgtaaaag gcacgcagaa gaagttgata tcaccgcagt
    17281 aagcagtgat tatcttggca agcttttcaa ctttcatctt cgctctgtct gaagtatacg
    17341 gtggagcttc aaagtggata gcggatatta tcgcaccacg ctttgccatc atatagcctg
    17401 caacaggaga atctataccg cctgagagaa gaagcattgc ctttccgctt gagccgacag
    17461 gtattccgcc agcgccctcg atagagcctg cgtgaacata agcgttggtt tctcttatct
    17521 ccactgttac tacaacgtca gggtcatgaa cgtcaacttt aaggtgtgga aatctttcaa
    17581 gtatctttcc gcccatttct atacatatct caggggactt gaatgggaac ttcttatctg
    17641 aacgctttgc gtcgaccttg aaagttctcg cagtttccat ttcgtcctcg atatagtcaa
    17701 gtgactttgt gagtatctcg tccatatcct tttccagaac gcagcagcga cagagcttaa
    17761 ctataccgaa aattttcgac agcttttcca ccgccatgtc aacatcgctg tcatcattaa
    17821 gtggctcaac atacaatgtg gactgcgcct ttgatacgct gaactgtccg cagcttttaa
    17881 gtcttctttt tacgctcttt gtcatcatgc tctcgaagct tgatttgtta agacctttca
    17941 gagctatctc gccatatttg cagagaatga tttctttcat aaattttatc ctttctttac
    18001 gaaaactgca caaaaaagtc ctcgtcaaaa acctgtcaaa agcgaccttt ttcgccgttc
    18061 ccctttataa atagtacaaa acatttgttt gtgtgtttta ccttaccttg cacaggtcat
    18121 tctgcgcaga ctttatgccc tccataagag cgtcgatatc ggcctttgtg gtgtcgatgc
    18181 taaagcttat cctgatagtg gagtcaaggc tctgctcggg atatctgaac tcttttagca
    18241 cagagctttt cttgcccttt gagcaggctg agccgcttga aacatatatc tcacgctttt
    18301 caagatagtg gagaagcact tcggacttta acccattgac ggcaatgctt gtgacataag
    18361 gcgaaccgcc ttttcgtgaa tttacgaaaa catcgccgtt ttcgttacac ttttctatga
    18421 gataggcttg gatgtcagcc gccttttcaa agttcgcaga aacattcggc gcaagctttt
    18481 tcaccgccgc accgaaaccg catatgagcg gcactgactc agtacccgaa cgaaaacctt
    18541 tttcctgtcc tcccccaagc ataaaaggcg gtatatgaat gccctttttc acatacagcg
    18601 caccgatacc ctttggagca tatattttat gaccgctgag agatatcata tcggcattca
    18661 gctttttggc tttcacagga attttcataa atccctgaac gcagtcgcag tgagtaatca
    18721 cctggggata ctttttcttt atcatagaaa aagctttttc cacaggaagt atgtagccgt
    18781 tttcgttgtt cacaagcata cagctaacaa ggaaagtgtc ctcgttcaca gcggcaaaga
    18841 tgtcacgagc gtttatctcg ccgttctcgt caggggatat ccttatgacc tcacagccca
    18901 tatcttcaag ctcctgggca ggatttttca cagcagggtg ttcaacagag gatatgacaa
    18961 cgcgcttttt acgttttccg tgagccttgt aagccccgaa aatagcggta ttgttgcttt
    19021 cagtagcacc cgaagtgaaa tagacctcat caggggtaac accgagagcg tcggcaatga
    19081 tctcacgagc gtcagaaaca agaagctcag cgtcaagacc tgctctgtgc agagaagagg
    19141 ggtttccgaa gacctccaag cctttcatta cagccgcttt agcttcatca caaggttttg
    19201 tggtggcggc attatcaaga tatatcaatt atcacaagtc ctttcatgtt ttatcgcaat
    19261 tatttttatt ataacatata attgtgaata atgcaaacaa atcgggaaag tgaggaatga
    19321 ggagtgagga gtgagaaatt atggtatcgc ctgcggcgat gtatttttac atagcacaat
    19381 gcggcacggc attttcagcc gcaccgcaca gaacactatt tatcacagtt acaattactt
    19441 ccccatgaag ccattgaaag cctgatataa tatccctgcg cttctccgca aacaggacac
    19501 ctctcagggg cttgtgtgcc ctccacgata taaccgcagt tcaagcatac ccacgtttca
    19561 gactgggagg aagagaacag ctttccctct ttcataagct tgccaaatgc cgaaaaacgc
    19621 ttgccgtgaa cattctctat cttggcgata ttcgtaaagt cctgcgctat ctttccatag
    19681 ccctcctgcc ttgcgacctc agcaaacgca gggtaaacat cttccgattc ctgacgctcg
    19741 ttgtggtctg ccataccaag caaggtacac atatcctccg aaatatccac aggataaccg
    19801 ccgtcaacaa acacgctctc tcctgacagc tctttgagat gttcgtagaa tatctttgcg
    19861 tgaactttct cctgatcggc tgtgaatttg aacaggtcgg caagcacata cagcttctgc
    19921 tgtcttgctt tttcctccgc cattgtgtag cggtttcgtg cctgactttc ccctgcaaag
    19981 gctctcataa ggttatcctt tgtctttgaa tttctgaaat ctgacatttt tttatcctcc
    20041 tactttatac agtcagtgtt cctgacttgc tttccacaag aatgagtatc tgctcctcct
    20101 cagcggtctg tgcatttata tatacaagca cgttcctgcc gttttctgcc ctgcacctga
    20161 actcataaca cagcttctcc tccaaaccgt cggtagggat aagtgcaagc ctgccggata
    20221 ctatttcaag gcgcggggac acgctctctt tcgctcggct ttgggatata tccgcttcgg
    20281 ggatatctct gtcatagtgg ttcaccaaaa agccctttgc gtcaaagcct aatatctcac
    20341 cgtcgtccat tgctactgat acctttataa ggtcggtgta acagcacacg tcaaggtcgt
    20401 tgtaggcgaa attcactgtg agaacgttgt cgatattctc atagtaggtg gttttcatgg
    20461 acagtatgcc gaggtcatcg agaaaatcct ctgctgcctt tactccctgc tcgtttgtaa
    20521 gctctgtgct ttcgctgata cggcttttga ggaaatatga gatatatccc ccctgcttcg
    20581 tcacctcgca agccaccgtc ttatcctcgt ttgaaaatct ccagcatggc attttgccct
    20641 ctacctctga gatatctgtg aacactgtgg agttcatgtt aagccccagg gaacagcgtt
    20701 caagggcttt ggtctgtgaa acgttctctg cctcagatgt catgcgtggg gtacgctcca
    20761 tgatattgtc tgagaatggg ccgtcataaa taaggcttgg atagctttca aagcttccct
    20821 caaagtctga gaagccctct gtcactgtgg gcgggtcgct ctgctgtgag cctgtttggg
    20881 tgagggctat ctcaccgctg ttcactgagc tttcaagctc ccacatatcc tcggaaagtt
    20941 ctttggaaaa gtcatagagc gaggatatgt tctcatactc ctcgtctgtt atctcctcgc
    21001 cgttcatgag cctctcgctc actgccatgg agtagtttcc tacctgtgag agaaatttgt
    21061 aggtgttttc aagctgcagc tcttctatgg gaagctgtga cagagccgcc tttgccgagg
    21121 acgcttctct gtaaagcttc accgcaagtg actggtgcat ttttcctgag cctgcataaa
    21181 gctccttttc aagagtatcg ctcacgttct ctactgcgtc gcacaggtcg cccatggctc
    21241 ttgaataggt gtagctcaca ctacgcaacg cccttttttc actgctcata agttgtatat
    21301 ttctcaccgc aagcacaccc acgacagcca ctgtgaaaga cactatccta actgcgtttc
    21361 gctttgatat atccattttt tatgctctcc ttttcttttt tctccattat tatgccagct
    21421 aaaatgctga ttattctcat taactgtgcc ttgcagtgcc atttcaggca tatcatgata
    21481 cacaaaattt ctttcctaat cttgtgtatc atgaacatct ttatttactt attattacat
    21541 aaatctcaaa ataagtgtaa tgatttaact ttaaaattac tgaattgtat ttatttgtat
    21601 aaacgcaaat ttgacaacgt tcagaaaaan nnnnnnnnna tgtgtaaaaa ttatcgtgta
    21661 aaatggcagg aaaaatgtta actctgccta caattctacg ggaggaaatt atgaaattta
    21721 ttccaaagct gactggcgtg gttttgtcag cggcaataat ggctcagtct gttccattcg
    21781 tgtatgcggc agagaaaagc attgcgtcaa gtgagctttc aaatgatatc cagatatcaa
    21841 agcctgaata tgctacaggc aatatggagg ctgatggtct tttagagcct gtcactgagg
    21901 acgaccctga gtatcataaa tatcttgaaa acaacgaccc atacggcatt atgacaatgt
    21961 ctgctctgtg gggggctgac agccttactc atcaaaacag attcagcggt gtttcaaagg
    22021 tctatggcat tgacgtttcc tattatcagg gcaacataga ttggaaaaag gtcaaaaaca
    22081 gcggtgtgga atttgttatc ataagagtag gctaccgtgg ctatggcagt gcaggaacgc
    22141 ttgtggaaga ccctaggttc aagacctatc ttgacggagc taccaaggca gggcttaaag
    22201 ttggcgtata cttctacacg caggcgataa ccacagccga ggctaaggca gaggctaagt
    22261 tcgttcttga caggataaag ggttattctc tgcaaatgcc tgtttattac gatatcgagt
    22321 ctgttgacta tgacactggc agacttgaca gcgcagggct ttccaaggct caaaagaccg
    22381 ctctttgcac agctttttgc gacactatca taaagtcggg ctacagcgca ggtgtttatg
    22441 caaactacac atggctaaac tactacattg acggagcagg gcttggcaag aaatatccta
    22501 tatggctggc tcactacaca agcaacacta attatgacca acgcatggat atgtggcaat
    22561 attcaggaag cggtactgtt tcaggcattt cagcctacac tgacgtgaac gtatggtatt
    22621 cgggcaagct tccactttac gtgtccgacc ttatctcagt gaaaaacacc tccacctcca
    22681 acacatttgc ctggaatggc gctcctgacg caacaggata tgaagtatat cagggaacaa
    22741 gccctagcga ccctaaaaag aaaaaaatag gggatacaaa gaatacatat tttacaaact
    22801 ccaacaagtc cacaggcact atgtacaaat acatggtgag agcctacagt gacgcttctg
    22861 gcaagcgtgt ttacggggat tactctgatg ttttcaccac ctgcactctt cctgcgaaca
    22921 tctcaaagat atccgcttct gcaaggggaa cttctgtcac cattagctgg ggcaaagttt
    22981 caaaggcgac cgactacatc gtggagcata atgtgaacgg tgcatggaag caggtcggca
    23041 caacttcaag cctttcatac aaggtgaatg gtatcacaca gaacggcgtt aataagttca
    23101 gggtaaaggc gagaagaaac tacagtggcg tatactacaa tggtggctac acatatgtaa
    23161 acgctgaggt cactgacata ccaagtacag taacaggcat aagaagcact tcaaacactt
    23221 ctacatcaaa cgccataaca tggaacgctt ccaaaaaagc ggaaggctat gagatctatc
    23281 agtggatagg tactaacgac agctacaagc tcataggcac gaccacgtcc acaaagttca
    23341 caaactcaaa gaaaagctca ggcacgatgt acagatacaa ggtaagggca ttcaacactg
    23401 ttgacggaca gcgtatcgag ggtgcatatt cctctgagct taccacctgc actcttcctg
    23461 caaacgtatc attctccctt tgctccactg acgttgattc cataacactc aactggaaca
    23521 aggtatcaaa ggctacaggc tatcaggtag agatgtacac aaacggaaca tggaaaactc
    23581 tctccaccct ttctggaacg agctacactg caagcgatct ttcacagaaa acagcttatc
    23641 gtttcagagt gagagccata agaaattata actacataaa ctactacggt gactacactg
    23701 aaaaggacat caccattcgt cctgcaaaca caccaaaggg gctttcctca tcagtgaaca
    23761 catcaagttc taacactatc acatgggaaa gcgtgaatgg agtatctggc tacagcgttt
    23821 atcagtgggt aggaacgtca gacagctaca aaaagctcgg cgacactgct tatccatact
    23881 acaccaactc aggcaagtca tcaggcacta tgtacacgta cagagtaaag gcatattatg
    23941 taagcgacaa tgttatgcaa tacagcaagc ctgcacaact tgtcacctgc acacttcctg
    24001 caaacgtcac tgtcaaaacg gcaaagcgtt cgggaagcaa catctcactt gcatggaaca
    24061 aggtatcaaa agccacaggt tatgagatat atgttaagtc aggacgcagt tggaagaagc
    24121 tcaccacaac atcaggcaaa agctacactg caaaaaaact gtcaggcact aaaacattca
    24181 gaataagggc atacagaaaa tacaatggtg tgaactatta tggtgcttat actgaaaaga
    24241 cggttaaata gtcactcgcc gtgacgggca ttttacgcca cacattgtga tatgatacaa
    24301 atttgcaccg ctgaataaaa atgaggcgct gtaaaaaaag cgcaacaaag cccgtggatt
    24361 ccctcgatat ggaagccc
//
LOCUS       DAWEMR010000013        23964 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_867_length_23964_cov_10.400017, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000013 DAWEMR010000000
VERSION     DAWEMR010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23964)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23964)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23964
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_23964_cov_10.400017"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    21011..21094
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23143..23152
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6483 a         5500 c         4518 g         7369 t
ORIGIN      
        1 gcaaacgccg ctgtgagtgt tcctgcaagc aattttttca tagcgattcc tcctaattat
       61 catttaaggg ttgtaattct atcagaatta tgctataatt atacataaca ttttagaggt
      121 tggattgctt tttctctgta tctatagtgt aacatagccc tattagttat aaaatagatt
      181 cagaatgaga tttgaaattc tttcattagt tatggatttg ttttgaatgt acttactatg
      241 ggaggcatta tgtctaaatt attttttgat tcacataaat attttgattt tgatactcaa
      301 ttactttata ccgatgatat tcctaaggaa gaggcgttga caggaatccc atttcgcatt
      361 ttagagtatt tagctaaaaa cgcacctctt cctttatctt gtgatcaaat acgccaagaa
      421 tgctgggaga acgacaatgg tacaggtgat gtaccatatc atataagtgt gttaagagaa
      481 tatattgagg acataaagat aaaaggcacg agaaaataca cttatatcgt tacgagagat
      541 aaaaaatact attgttccat taaattaaca agcgatttta tttcagacgg cacgaatagt
      601 tttattactg atgagttgga atgtgataat aatagtgtag attatttgat ttccccatta
      661 gactattgta aacaaagagg cattaaaggt tttttagata gtggtcttgc tcctcaagtt
      721 ttaattaaaa atttaaaaaa caaaacaaac atttatattc ttacaaccac cggcgcaaac
      781 ttaataacag cgttgtcatc agatttcatt gcagattcgc ttgttcacgg cacttctttt
      841 actatattac taccgaataa gtattctgag tttatcaatg atgtggctga tattgaaacg
      901 ccttatgata aagaattatc aagagatgca tttgcaacac aatttgaaaa tataattaaa
      961 acgattaagc gagaagtgct aaaagcacag aatttgtcaa atcaaagtac agtcggatct
     1021 gtgtatattg gatgcgcatt tacatatttg cgtcaaacaa ttattatggg cataaatgaa
     1081 accgaagtat gggggtatca gtcaatgaca atgccacctg caagaactat taatggaact
     1141 acttcatttg tgttctctgg caatatcaat gaaaatacga tagccaaacg aacatataat
     1201 cacataaatg cattgattga tgaagctaag catagattct ctttttttga agtgacggaa
     1261 ttaataaatc catcaacatt taattttggc ttagaaaaaa caaatgcacg gacatattgg
     1321 gaatctttat atagaaaagc agatattaac atgaaaatac atatgcaata tgaagatgaa
     1381 cttattgagg ttgcagctca gcatccactt aatcatggcg agccagattc agaatttaaa
     1441 gaaaggttaa actttgcatt cgaattgtat aataagctaa gaaaaggcaa tcgaagagtt
     1501 ggaatatata ttcctggtag tgtacataca gcaaatggca tacaagacca gtattcacta
     1561 agttttgcag gggttaaata tctaacaaag cttggaatac ctatcgaatc cttattagga
     1621 gatgaatata atcaaaagta taagggcgaa aatggtgtat ataactctgc tgacgaatgt
     1681 tatgttgcat ctcagatttt tttaaacgga aaatataagt acattcatgt tgtttgttcc
     1741 ccaaatcaaa tgcttcgcaa acaattgttt tacatttctt ttggcgtaat tccgctattt
     1801 catacggttc caaataataa tccagctcat gatttgattt atgagttatt tgaagcgata
     1861 ccagatgtca tatataatga ccatacgtgg caagatgacg attctcctaa tgctattcgt
     1921 acaagaaatg aacgtaaacc caaatagcca ataaacaatg gtatctatat tatgtacaga
     1981 ttgctgcgct ataaaaatac agaacaggca gatacctcgt aatgaagtat ctgcctgttc
     2041 tgtttatagc ccgctgtata ggtctttaca taatatctgt tgaaaaactt ctatattagt
     2101 accgacttga gagggtacgg tgataaaata ttcagcacac agataatatg ccaatatacg
     2161 ggtgaacgct gttcacctct gcatatgtta ggctgaaaag actatttctt tttacgcttt
     2221 gcacagatcg ccaaacctgc tccagcggct attattgcgg ctgtaacgcc tgccgccgca
     2281 cctgttgacg gatttgttga cgaatcggta ttataattct ttgaagcctg agagcctgct
     2341 gttttgtcag ggttagagat atcatttgaa gtgttcttct tttctacata tgtaaagctt
     2401 gctccgccca caacgtcccc aagcttataa tcgtcatatg actctgcaag ggtgccttca
     2461 ttattaggga ctttgttttc gcccatggtg cttattgtta cagaaagctc atcatagtcg
     2521 ctgttaagct ttgcaagata cgcctgaaac tcctctgatg tggcttcttt tccctcgatg
     2581 gtgtatgaag tcacgccttt tttgctgtca tattctgcgg tgcagatatc agcggtgaga
     2641 cattcaccaa gacagctaaa aagctttctc actgcgtaat gctccttgcc gtcctctgca
     2701 tatctatcct ctgagaggta tgttttttcg ccagtctgct tgtttacata aggcttgaag
     2761 ccgaagcctt tctcaccgtc gccatggtcg ttcgacacat tggtatccgc caaaaagcct
     2821 gcattgccca taccgataac gtcataaagc cttaaagcag taccttcatc taagaggtct
     2881 gcaataccgc cgataacaaa gtcaggaacg ccgtcaaagt ttaaatcctc aaaccaactg
     2941 ttatagctcc aatcaacatc atcagcaatt tcagaaggaa cgtcaaccat catctcttta
     3001 agaagatcga ccatctcgac tattacgctt atcatatcgt cctttttgtt ctccgccgtc
     3061 atgacaacgc agttatactc gttatacatt tccagaagct tatcagcgtc agcctttgca
     3121 gtttcatctg atgtcagctc tgtattttcc acttcaagag ggataaatct gctgagggtg
     3181 gatttatagt tataatactc gctctcgctg acctctttgc cgtctgtttt gtaaacagtg
     3241 ctgtcagttt ccaaatcgta ttcttccgag caaagctcct tttcagtaac gcctttttca
     3301 gggtcaaagg tcatagtttt tatcttatcg ttacttgaaa aatatgatac ctcccctgta
     3361 taaagatctc tgtaaggcat ataatcaaag cccataaatc ccggaaaata ctgtccgttt
     3421 gcaacagtgt catctatctt tatctccttg ccgtccttgg taagaataga ataatgtctg
     3481 tcgccgtcag ccatttcacc gcctattata aactcaggca caccgtcata atccagatcc
     3541 atgaaccatg agccatagtc gaagtaggtc ttatcgccgt ctgcaacact gtccatactc
     3601 tttaaaatat caggcagctt gccgtctatg aaattgacga agtcttctcc tgcgtcctta
     3661 tcaagaacat aggaagcatc tgccgaagct gtagtgaaca tcatgctcgc tgacataatg
     3721 accgcaagcg ccgccgcact tatctttttt atcatacata tcaccccaca aaatagtttt
     3781 catacatata cctacttaat atttcataca tatatctact taatatataa catatatcaa
     3841 acaaaaagtc aagaaaaaaa ctgaccaaag cacacacttt ggtcagttta tcacttttta
     3901 gggaagggta tttagcaata ttgtattatt gcaggtcgcc tctgttaaat tctgaaagct
     3961 caagaccctc gtttctttca agcgcccagc ggatattcca ctcgcttgtg aaaagaagca
     4021 gatagttgtc acggaagtcc ttgacaagct ttgtgtcact tgaaagcttc aatgtcttag
     4081 ggtcgatatc cttgttatct atccagcgga tataagaata aggcagaccc tctttgaaga
     4141 ggtcaacgtt gtactcattt ttcattctgt attcaaaaac ttcaagctga agaacgccca
     4201 caacgcccac gataacttct tccataccgc tgtcaggctc atggaatatc tgtatcgcac
     4261 cctcttgtgc gatctgtgtt gtacccttta cgaactgctt tctcttgagg gtatccttct
     4321 gacggactct cataaaatgc tcaggtgcaa aggtcgggat agactcgaac tcgaactttt
     4381 tctttggcga gcagatcgta tcaccgatag cgaagatacc tgggtcaaac acaccgatta
     4441 tatctcctgc ataggcttca tcgattatct ctctttcctg agccattatc tgctgaggct
     4501 gagaaagtct gagcttttta ttgccctgaa cgtggagaac ttccatatcc ttatcgaact
     4561 tgcctgagca gatacgcagg aaagtgatac ggtcacggtg agctttgttc atattcgcct
     4621 gaattttgaa tacgaaagcc gagaactcag ggtcgaacgg atctatgata ccggcagagg
     4681 aattcctcgg agtaggagaa gttgtcattt ccaggaagtt ttccaaaaac ggctcaacgc
     4741 cgaagtttgt aagagcagag ccaaagaaca caggcgaaag ctcacccttt ctcacctttt
     4801 cgatatcgaa ctcatagctt gcgccatcga gaagctcgat atcatcgcag agagttgagt
     4861 ggagatcctc gccgataagg ctgtcaagtg acgggtcgga gaggtcgact tctgtagctg
     4921 ccacctgatg ctgtccaccg cttgcctcaa aagagattat atgcttttta tctctgtcat
     4981 aaacgccctt aaactctttg cctgagccga taggccaatt cacaggatag gtctggatac
     5041 caagctcaga ctctatctgc tccataagat cgaaaggatt tctggactca cgatccattt
     5101 tatttacgaa agtaaatata gggatatggc gcataacgca gaccttgaaa agctttctgg
     5161 tctgattttc cacacccttt gaagcgtcta ttaccattac ggctgagtct gcagccataa
     5221 gagtacggta ggtatcctca gagaagtcct gatgtccagg agtatccagg atatttatac
     5281 aataattttt atactgaaac tgcataacag aagaagtaac agagatacct ctttgttttt
     5341 ctatttccat ccagtctgaa acagcatggc gggagttctt tttacctttt acagcacctg
     5401 cctgagctat cgcaccacca tagagcagaa atttttccgt aagagttgtt ttacctgcgt
     5461 cagggtggga aattatcgca aaagtacggc ggcgtttgat ctcatcttca agcttaccca
     5521 aaattattcc tccatatttc attgcatttt taaacatacc ttgttagtat agcacagtat
     5581 cgcttaaatt gcaagagatt ttttatgaag aaagcggcgt ttgtagaatg aggagtgagg
     5641 aatgaggaat tatggtatcg cctgtcggcg ataaattttt tgataggggc gaacgctgtt
     5701 cgcctgcttc ttgtttgcca aacttgcgtt atcccccata tttggcttca agttctactg
     5761 ccgcaaaatc gcttcgctcg gtgttggctt caaaactcgg tgcggctatg tcattcctca
     5821 ctcctcactc ctcactcctc acttaaaccg ctcctcactt aacttcgttc ctcacttaaa
     5881 cagccgttga cattctccac aaactatgct aaaatcaaat tataagcaat caactatcga
     5941 acctacaaag gaggctgttt tatgaaagat ctgctcgaac ttatgaaaac cagaagaagt
     6001 gtacgaaaat acaagcctga catgataccg caggacgttc tcgaccgcat cataatagca
     6061 ggaacttatt ccgccacagg aagaaacttg cagtcaccta ttatcattgc tgtcaccaac
     6121 aaggaaatgc gtgacaaaat ttctgagatg aacagaaaga taggcggctg ggctgagggc
     6181 ttcgacccgt tctacggttc gcctgttatt cttattgtac tagctgacaa atcctgcccg
     6241 acttacattt atgacggaag tcttgttatg ggaaatctta tgcttgccgc tcacaacgag
     6301 ggcgttggaa gttgctggat acatcgtgca aaggaagaat ttgagtccga agaaggcaag
     6361 gagatcctcc gcagccttgg tatcgagggc gaatacgagg gcatcggtca ctgcgcactc
     6421 ggctacactg agggtgaata tcccgagggt cacgaaagaa aagacaattg ggtatactat
     6481 atcaaataat cctgacaaac tatctaacaa aaaacggagc ggtcaaaacc gttccgtttt
     6541 tttgttctta ttaatgagct gtcacgtcag aaaattctat gccgacctgc ttgccctcat
     6601 atgttgcgga atactcgaac gttgtaccaa caactgtcgc cttgccattg ctgtctatct
     6661 tgagcttgca cttcggaagc ttctggatat aaagctgttt tgtttcccca ggatagttgt
     6721 tgtcatatat ctgaaggata tattttctgt ggcaatcaga atcctgaata agtccgatag
     6781 cgttcactgt atgagtatcg tctatagttg taacaactgg tatgccaagg ctgagctggt
     6841 cttcaagctt ttcaaagccc ttactgtcgt tgttaaggtt atactctgac gaagaatcgt
     6901 cccactggag ggcgttaagt ctgtagagtg cctttgcgaa ctctgcgtca tttgaggaat
     6961 agccaccctt tattttgcca atgccgtttg ctatatccat atcaagcagc tctacctttt
     7021 tccagcggag attgccgcct gatattttcg taacgtcctt tacccagccc ttgttcttgc
     7081 tgtcatactt atagtcatca ttaagggtga gaatatcccc tgtgcaggaa tagtcgagat
     7141 actttgtaac gtctgaatat gtgcccgcaa aaaggtctga ggttatcttg ctaagcggca
     7201 ctctttcgtc atactgttta ccaaagtctg ttccgcttat gtcatagcct gcggcattca
     7261 cctttatgtc gctcttatct gacggagcga tatccccaag agaagtcttt acatttccga
     7321 gataccaatc tcttgccata acagccatgc cgaagtcaac acttgcggta gttgttgttc
     7381 tgaaattctt gaatgagaag ccgttcacct ttgggtcaaa gcctgtgttg taaagtgaat
     7441 aacctcttac gttatcgtca gcgttcgtgt atgtaacgat atcatagttc agtgtagtaa
     7501 ctatctgctt gtaaacatca ttaagggcat ttgcgtcgga agctgagtaa tactttccgc
     7561 ctgtggaata agcaagctcc tgaagccaat ctctgtcaac atctctgcca agaccaactg
     7621 tgagaacgat tatgtcctta tcctttgcag tatttgcaag gcttgaaacg cttgcaggat
     7681 taaactcgtc agactcgccg tctgtgagca tcacgatgat atttctgtat tttccaccgg
     7741 aagttgcctt gaactcgtcc atgcactttt taagagccgt ctggctgtga gtgccgttaa
     7801 atatctcttt ctcagacttt atcttatcga gagcttccgt aagcttcgtc ttatcatcag
     7861 tgaagtctac catctttttg tagcttcctg tgaacttgca aatgccgacc tgatagtggc
     7921 tctcaaactt gtcgataaga ttctttgcaa attcaagacg cttgaagtca acatcattct
     7981 cagaagaagt tacacactgc tcctttggat acatagagcc ggagttatca acgaggaagc
     8041 agatccttgt tgtggcttca tcgtttacag ttttctcaac acctaccaca taagtgccaa
     8101 gctctgtaag agaagcagaa actgtctttt tgctcttgtc tgcctttgaa tccacctttg
     8161 tgtaaagggt cgagcgtgaa tcaaatctca gcactgtaag gtcatcaaat gaatatccca
     8221 ttttactcag ctttgcagag tcgatatcaa aagttatctt tgccgagttg aacttatagt
     8281 cagaataagc gtcataagcc tttgtgacga tacttgcatt tgatgagata ccaaagagat
     8341 caagctcaac gagggtcgca ctggcagcgt ttgcgtcacc ctttatttca aggcggcagt
     8401 cccctgcatt tttttccact gtaagctctc tttcagcgtc attggtcttt ccgtcagact
     8461 ttgactctct cggatttgtg cctgccataa gctcatagcc gtcaagaaga ccgtcgccgt
     8521 cagtatcggc ttcaataggg ttcgtgccta gctttgtctc atcgccgtcg cttattccgt
     8581 cattgtcagt atcctcgctt tgaaggttag tgccatgacg tacctcatcg gcattcttta
     8641 tgccgtcacc gtcccagtcc tgatcgtcag aaaactccga ctccttgaag gtagtgctta
     8701 tatcaagctt tgttatgacc gattttgcca tggcgatacg gttattttcc cttattttca
     8761 gacccacgac aacacaggca gccacaagga acgcaagcac tatacacagc accagcaacg
     8821 ctatcttctt tttgcgcttt cctctgttca tgctctgaac aaaatcattc gtatcagaaa
     8881 aatgtgattt cataaaaatt aattcccctt atcagatcac atatttcacg atattaatat
     8941 tttagcatat atgtacctca tttgcaagca aaataaccta gaataattca caaaataaac
     9001 tgttcttttt tgtttacaaa cagtatatct taccaaaaca ttaacaagat aattctttta
     9061 ttattaacct aaaataaatt aaaaccttct ttttccacaa aaaagggcag cccctctcgg
     9121 agctgccctt tggcataaca ctatcactct tctgtaacag tattataaat aacgttcaca
     9181 acgaagtcct tgtcataatc tacctgcaag atagaatcag gctcaagctc gtctgcaatg
     9241 atcttctttg cgataagagt ttcgatacgt ctttggataa acctcttgag aggtcttgca
     9301 ccgaagttct gatcgtatcc ctgatttacg atagcttcct tagcggcgtc tgttacctct
     9361 atgctgagcc tcttgtcttc aagacgcttg ccaagctctg caagcataag atcaactatc
     9421 ttgtatatct ccgtcttcat aagcggcttg tagtagatta tatcgtcaag tctgttaaga
     9481 aactcaggtc tgaactgctg tttcagcaga gcgtccacct tgtcccttgc ttcctgagat
     9541 atctcgccct caggtgtgat gccctcaagg atataaggag aaccaaggtt tgaagtgagg
     9601 attatgatag tgttcttgaa gtcaaccgtt ctgccctgtg agtctgtgat acgtccgtcg
     9661 tcaagcacct gcaaaaggat attgaaaacg tcagggtgag ccttttcgat ctcgtcaaga
     9721 agaactactg aataaggctt tcttcttaca gcctcggtga gctgaccgcc ctcctcatag
     9781 cctacgtatc ccggaggcgc tccgataaga cggcttacgc tgaatttctc catgtactcc
     9841 gacatatcta tacgaacgat attgttctcg tcgtcaaaca aagcctgagc aagagttttt
     9901 gcaagctctg ttttaccaac gcctgtagga ccaaggaaca ggaacgaacc tatcggctga
     9961 tttggatttg cgataccggc acgagacctc ataatagcct cagacacacg ctcaacggct
    10021 tcgtcctgac cgataactct ctcgtggagg atatcaggca ggtggagaag cttctcacgc
    10081 tcgccctcca taagctttgc aacagggata cctgtccagc ggcagattat ccttgcgatc
    10141 tcttcctctg ttaccttatc tcttagcaga gtgttctttg tctttgactt ctctgccttt
    10201 tcttcttcct cggcaagctg cttctgcaag tcagccattc tgccgtattt cagctctgca
    10261 agcttgttaa ggtcatattc acgctctgct ctctcgatat cctttgagca ttgctcgata
    10321 tcctcacgga gcttctgcac cttagagata gcagacttct cattctccca tttagctttc
    10381 atctcattga actgctcacg catttctgaa agctctttct gcacctcatg gagatgttca
    10441 actgcaagct tatcagtttc ctttttaagg acagtttcct ctatctcgtg ctggagtatc
    10501 ttacgtctta tctcatcaag ctctgtaggc attgagtcca tttccgttct gatgagtgcg
    10561 caggcttcgt ccacaaggtc gatagcctta tcagggagaa atctgtctgt gatatatctg
    10621 tttgaaagaa cagccgccgc aataagcgcc tgatcctgaa tttttacgcc gtggaaaact
    10681 tcgtatctct ctttaagtcc acgaagtata gaaatagtat cctcaactgt cggctcgtca
    10741 accataacag gctggaaacg tctttcaaga gcggcgtcct tttcgatata ctgacggtac
    10801 tcgttaagag tcgttgcacc gatacaatgg agttcgcccc ttgcaagcat aggtttgagc
    10861 aggttgcctg cgtccattgc gccgtctgac tttcctgcgc caacgatatt gtgaagctca
    10921 tctataaaga ggattatcct gccctcagac ttctttatct cctcaacgac tgccttgaat
    10981 ctttcctcaa actcgcctct gtattttgcg cctgcgataa gcgcacccat atcaagtgag
    11041 aaaacctgtc tgtctttcag actgtcaggc acatctccgc tgacgatacg ctgagcaaga
    11101 ccctctgcaa tggcggtctt accaacgcca ggctcaccaa taaggcaagg attgttcttt
    11161 gtttttcttg aaagtatcct tataacgttt cttatctcac tgtctctgcc gataacaggg
    11221 tcaagcttgt tctgtcttgc aagctccaca agatcctgac cgtacttttt cagcacgtca
    11281 tatgtttcct cagggttctg gctcgttacc tgagcgtttc ctcttacagc ttgaagaact
    11341 ttcagaaact ttgtttttgt taccccaaag gtcttgaaga tctcttttac tgcgctgtta
    11401 ggactgtcca ttatgcccat aacaagatgt tcaactgaaa cgtattcgtc cttcattctt
    11461 gaagcttccg actcggcttc cgcaaaagcc ttgtcaaggt cgcttgaaat gtatacagag
    11521 tcctgcgcac gtccgcctac ctgtacctta ggtatatcgt caactgctct tgtaagagcc
    11581 gcctctatat tttcaggggc tatatccatt tttacaaaaa gctgcggcac aagaccgttt
    11641 tcctgtgaaa gcaaagccaa cagcaagtgc tgctggtcaa cagcctgatt tgagtttctt
    11701 gaggcaatgc tctgcgcttc ctgcaaagct tccaatgatt tctgtgtcaa tttctgagcg
    11761 ttcataaaat tacctccctt atcatcttaa aaagatatat gctcacagca ggcggaatgc
    11821 accgcccacc gtttaaagaa cgacaacgcc ttttttcaga tatctttcat acatttcaga
    11881 tacctttctt gccgccgttt caggttctgc agcgttgtca aagtaaagct gaatacagct
    11941 gtccatacag cttatataat caccgatagc gttcgctata tcctcgtcgg tcagctccga
    12001 ctgaggcggt gcgtaaaaat ctcttgtaaa tctgccctcg gctgtttcat aaggcacgcc
    12061 agtagtgaaa agatacgaaa gatatttttc ttcgagcctt gcccacagct taaaaaagct
    12121 gtcaagcatt gaaatgagct gtgtgctttg ggttctgaaa gatactttca gcctccccac
    12181 ctttccgtga aaatcacggc tcagctctat gctgtatcgt atggtcggct tatatttata
    12241 tctcaaagga ctttttatag acatcatagc gtcagagggc tgattaaggg tctgaaacct
    12301 gctgtccata agctgttcta ttttagagaa tatttcacgc attctttttt caggggtgag
    12361 aagctggaca cgctctgcca aaatctgatt tatcagattt gaacggctgg tattcatgga
    12421 atatgccagt ctgtcgatag cctccaccac atcgtctgca agcaccagac tatacacgct
    12481 cttgttcact ttgctctcat tcctttcttt aaggcgttcc catatggcgt cgccctttat
    12541 cactgtactt attataccac attctaataa tttgtcaata ggattatata agtttaatta
    12601 cgaattatat agatgcacaa tagaatgtat gttctgcacc tctaatttgt gcagttttat
    12661 tgaaaacttg tcaaaagtgt gatataataa cagataccaa gttggaaagg cgtgataaaa
    12721 atgctcaaag gcgttatatt tgatatggac ggactaatga tagatacgga aaagctgctc
    12781 acaaggtttt ggtgtgaggc ggcgaatcat tacggctatc caatgacaaa ggaacacgtt
    12841 ttgggcatac gctctcttgc agcgaagtat gctatcccac atctgaaagg cattttcgga
    12901 gaggattttg attactacaa agttcgtgca aagcgcatcg agcttatgaa cgagtttatt
    12961 aggcaaaacg gcatagaaaa gaaaaagggg cttgatgagc ttcttgacta tcttgacagc
    13021 acagggctga aaaaagccgt tgccactgcc accgacctcc agcgcaccac gctgtatctc
    13081 agctctataa atatcattga cagatttgac aagcttatct gcggaaacat gatagaaaac
    13141 ggcaagcctg cccctgatat ttatctcgcc gccgcaaagg agctggggct tgaccctagc
    13201 gagtgcattg ccctcgagga ctcaccaaac ggcatacgct ccgcccacgc cgcaggctgc
    13261 aaacctgtta tgatacccga cctttcacag cctgacgagg aaacaaaaaa gcttctgtat
    13321 gcctgctgtg atgacctttc caaggtcata ggcgtgatac aaaagctttt aagatgaata
    13381 ttctgccaat tcatgtaggg gcgaacattg ttcgcccgct ttttgtaaaa accctataaa
    13441 aattgataca atgttacacg aattgacaca aacgctacat agggatagta aaaccgatag
    13501 ctttatcact tcgacgggcg aacgctgttc gcccctacac tgactacact gatttgcaaa
    13561 ataaaataca aaaatgcgga tacctccacg atatccgcat ttatttttta tcctatctaa
    13621 ttaatcctcg acctcagaaa cagaagacgt gtcagtgtcc gtcttgctgt ccttgtcttt
    13681 attatcagaa gccttgccgc ccagatactt ttcataaata gctatctgat cctcaacaga
    13741 cttcagctcg tccttagact ttgttgtaac acaaatatat ttgtgtccgt tatcatcaag
    13801 tatagttgaa agggtgtact ttgcttcgtc ggtgaagcct gtctttccgc cctcaatgct
    13861 tataccgtcg tcagcctcgc cgtcaccgtc acaatccaca tagtagccct caattctctg
    13921 tgcaacaatg cttgtaagct cgataccgtc cttgtgctgt tttgtctttg aagttgtgta
    13981 ctttgttgct gtaagcactt cacggcaagt ttcgttatcc atagccgcct ttgtgataac
    14041 tgcgatatcc tgcgctgtgg aataatgatt tttgttgtgc agaccacttg cattcacaaa
    14101 cttcgtgccg gtaagaccaa gctcctggat cttagcgttc ataagctcaa cgaacttctc
    14161 ctctgagcct gcaactgcgt ttgcaagacc tgttgtgccg tcagcaccgc tcacaaggat
    14221 agctgaataa agcaggtctt tcattgtaac agtttccccg gcttcaaagc ctgctcttga
    14281 tgcgttgtcc tcgataagag ggtcgatatc atcgcttgtg aacttatatg tagcgtccat
    14341 atcctgaata ttctctgcgg ctacaagaag cgtaagcatc tttgtaagag aagcaggata
    14401 tacctttttc tcgtaattat aacctgcaac tatctcgtta gtgtcagcgt caacaagtat
    14461 agctgagttt gctgtcatac cctttatatt tatatcctga taatttgcag acttcacagg
    14521 ataattgctc acaggctctt ccacaactga tgaaaggtcg tcaacctctg aaacctcctc
    14581 agaagagctt tcctcaggtt tgctgttcac cttaacaact gcgtctgcca ccttgcttgc
    14641 ctgaatggta tatcttcctg ttaccaccag tgccgctgaa ccgacagcca caagcattat
    14701 aactataagc acagcaacta tagctgcacc gccctttttg tttgtcctct tcatttttac
    14761 aacaattcct ttcctagtgc ttatactgca cctatctcct ctttttcttc cttatgacca
    14821 tatttcgctt catactcgtt aagagtatcg tgacaatatt taagtatatc tcttctcgtc
    14881 acaataccta taaaaacttc cctgtcatca ataacaggca caaagttctg atccattgaa
    14941 agaagtatca gatcctcgat agtagaataa acatagacgg gtttcattct aacacgtctt
    15001 gttatctcac ttatcctata tttctccagc tcacgcatat cctgaatgcc attattgagc
    15061 atgaaccaca gaaagtcgcc ctctgcaagg gtctttacat atctgccctc acggtcgata
    15121 acaggcacag cggtaaagct gtgatttttc attttctcaa gagcctgcct tgctgtgttg
    15181 tcatcataga caaaatccac gcaagccttc ggcttcaaaa actttagtat attcattttt
    15241 atatatatcc tcttgtaagc atacgttttt atgcggtaaa gaccgcacct ataattatac
    15301 caaaaaaacg catacaagta aagcatagag agggcatttt ccgccctgcg ttttcaacaa
    15361 atctccacgc tgaaattgtg cattttgact tactgctgtg cagacttgta gccgtatgtg
    15421 taaacaaggc tcacaaagct gtcataggat atagtcttaa gatcgtccgt ctgatatccc
    15481 cctgcactga gattgtctgt tgtaacgcct gctttgttga agataccctg acaatagcca
    15541 agcctctgat cctctgacat actgtcaaag tccacaagac ttgtcgcctg agatacgtca
    15601 aacaggctgt cgtcagcgtc ccctgaaagc tcgtcaacag tgtattctga aactgtatca
    15661 tctgagcctt caatggtaaa ggtctgcgtg tattttttta agcttccgtc agatttatca
    15721 aaatacaggt cgatagcagt aatgtatgtg tcgcccgtat aagtgtactc ctcgatatcg
    15781 aaatcatcag cctcatcact gtttatatga ccataggttc tcggcaggtt ttgcttgact
    15841 atgctttcaa gaacgccgtc aagctccgtc acgtctgact tgtgataaac accggtcacg
    15901 ttatcgtaat catatcctgc tccgtcaaca tagataaatc cgctggtgcc tatatccgat
    15961 gtgactttca gatagatgtt tccgcccttt ttggctctta ctacctcctg ctttgagccg
    16021 tcggcctctg tgcaggtcgt tttcagagtg tagtcaccgt caaagatcgc agcagttttt
    16081 gtaataagct caccctgctc gttcaatacc ttactgtctg catcactctc gtcacccttg
    16141 ctgtcctcag actttgcgct gtcggcattg tcggctgttg aaacgttcgc cttgctctct
    16201 gtggcagaag agctttcctt actatccttt gaacagcccg ctgcacacaa gcataccacg
    16261 caagcgcaga gagccgcaca tattttcttt atatccatat gtaaatatcc tttcagacca
    16321 tgtactatat ttctttttca agacaatact gctcttaaca ttatacaccc cttaaacttt
    16381 tatgtcaaga gataaggcat agcgtaaaga tcggtgagtt atttgctatt ttaccttatt
    16441 tacgcacata aaaatcacaa aaatccccta tcaatgtata ctgtgagttc atgctttttt
    16501 cacaaaagtc tgatataata tttgggtatt ctgacgaaag aacacctaaa gaagaaaaat
    16561 atatgtgaaa gaggaagtaa aaatggaagc aggtctgata atcggaaacc aaataataat
    16621 aatgttcatt atccttatta taggcgcatt ttgctattta aaagggataa tcacaaagga
    16681 cggtacaaaa cagttgtcct cattcgcact gaatataatc aatcctgtgc ttatattcat
    16741 gtcctatcag tcaaaatatg acaagagtct tgtgcatgga ttgctgtggt cttttctgct
    16801 gtcagcgata tcctatgcag tgactatctt gctcacctat gttcttataa gaaaaaagac
    16861 tcccgacctt gcggtggagc gtgtttcgtc aatatattca aactgtgcat tcatgtgcat
    16921 accccttata aacggcattt acggctcaga gggcgttttg tatcttacag cgtatcttac
    16981 gttcttcaac gtccttatct ggacgcacgg acttatgaca atgaaaggcg agcgtgactt
    17041 ttcttctctt gtgaaagccc ttatctcacc gtcagttata gccgttgtgg tcggtcttat
    17101 ttgctatctt acaaatgtca gactgccgaa agttcctgca acagctttgc agtacatagc
    17161 agatatgaac acgcctatcg ccatggtgat agcaggtgca acagccgcac agaccaacgt
    17221 tttgaaagct cttcaaaacc gccgtatcta ctacgtttca ctgtgcaagc tcctaatact
    17281 ccctgtggca ggcttctttg caatgtggat acttgatcct cctccgatag ttcttatgac
    17341 agttaccata gccgccgcct gccctgctgc gaccatgtgt acaatgttcg ctgtaaacct
    17401 tgacaaggac cctaaaacag cctcagagat ctttacaatc accactattc tcagtgctgt
    17461 aactctgccg cttataacta tctttgccga cagacttaca tatcatattt aaagtcagcc
    17521 taagcaaaca tatccatata aacaaaaaga cctgtcagca tttcgctgca caggctgtga
    17581 aaagataaat aagctacgct ctgcaatgct ttccgaaaac ctcggagagc attttttatc
    17641 actctttttg ctgcaccgca ggtaattcta taaaaagcct gccgcacaca ctgatcccct
    17701 tcatgacaac accgtttaaa gagatttcaa acgtcttgcc tttataattt tttatccacc
    17761 catagtatac accttacttc atcggtgcct cggataataa aagtataaca tataatcaat
    17821 tcactgtcaa tatttctttg aacaaatatc atataaattg tatatttcaa taaaacaacc
    17881 agaaaattta tagcgtttta cacaaaattg tcagaaagca ttctcaaaca agacacaatt
    17941 tctccgctat tttacttttt tctgcaaaat attatcctcc gccctcataa aaaatctgta
    18001 tacatctcca agagaagcaa ggtcactgca tactgtgtcc agcaagttca cgttgaaaag
    18061 cagcttaggg tcctcactgt caaagcttac accgatatcc tttttattga gccacaaagc
    18121 gtcctcacgg ctggcatttg gaaaatggtc acgcttatac tcgtcaccga acaaaacgaa
    18181 cttgctgttg tctttcagcg ccttgtaagc ctttttatat tcctgcgccc ctgcaagtat
    18241 catcgccctc atttcgtcca tgctttctct gtcggtcttg taatatccac aaccatagcc
    18301 gaaaccgtgg gtagagatat agaaataaaa ctcaggcatg gggtgatatt cgcttttgtc
    18361 acgctggcat ttcaaagaac accacacgct ctctctgaat atgggtccgc ccttgttgta
    18421 acgtgcgtct ttgtaaagcc ttgatatctt tttcgggttg catactatcc tgctgtcaat
    18481 atcgttcatt ataggcgtga gcgcagtgat aagctccgca aaaggctctt ctatgagtct
    18541 gtgataatcc gccttatgct ccttgaacca ttccttacta tctctcgtat gattttcaaa
    18601 caaaaagccg atagcttctt cactaaatag cattttttct ctctccttga tgtttctaaa
    18661 cttattatag tatatcacga tttgcatttt taatcaagaa aaaagagcct gccaaagcaa
    18721 gctctcttcc aaattgattt tatgaatgaa gtcccatttc gtaagcggtt ttcatgtgtg
    18781 cactgctctg cacttcgccc ttgtcccaca cgcctgtgcc gtaaaggata cccttttcaa
    18841 ctgccccgtc aaggcagtca agagaaaatc cccttatgct ctcaactgtc ttttcaaggt
    18901 tagcggcatt ggtatcagcc gcagtgagta tataatacac ttctttgttt accatttcag
    18961 tgtatctcgg cactgttctg tcaatgagtg tcttgagctg agcgtccatg gtgtagaaat
    19021 aaacaggcgt tgcaaaaacg ataacgtcag cgttcaccat tttgtccatt atctccgcca
    19081 tatcgtcttt ctgcacgcac ttgtgagtgc tgttgcaaac gccgcagcct gtgcagtagt
    19141 tcaccttaaa gtttctgaga aatatttttt ccaccttgtg tcctgcctgc tgtgcaccct
    19201 ttatgaactc atcgcaaagc acgtcggaat ttccgccctt tctagggctt gcggatataa
    19261 caagtatatt tttcatattg tcacctcaaa tatcaaagag tttttataac gtcctcagct
    19321 atctgctcag gggtaagatg attttctttc agcacctcgg cagggtcata gcggtcatag
    19381 aacttctttt caagaccaaa gcaaagggtc tttacatcac tgtcgccata aaatcttgct
    19441 atcttctcgc caaagccgcc ctcaagaaca ccgtcctcaa gagtgataat ggtcttgtgg
    19501 tcagccttaa ggctttcaag catttcattg tcaatgcctg tgatgtagta agggtttatt
    19561 acagtagcct tgacacctgt tttttcctcg ataagctttg cggcttcaac tcccatgccg
    19621 tagaatgaac ccagtgcgat aacagccacc tgacttccct tctgtgtcat ctcatatttg
    19681 ttgagctttg aaagatcctt tgtgaaaggc ttgccgtctg aaacaaggct tgcaggaact
    19741 cttattgcaa cagggtgttc gttctgttca agggaccaat ccagcattgc aagatattct
    19801 tcctttgtta caggtgcaag ataaacaagc tcagggatat ttgatatcat cggtatatcg
    19861 taaattccaa ggtgagtaac gtcactcatg ccgtaaaccg aagccgcaaa aacagctatt
    19921 gtaacaggac ttttatttat gcaaacgtcc tgagaaagct ggtcgtaggt acgctgtaag
    19981 aacgagctgt aaacgccgta gaaaggcttt ccgccattct ttgctatacc tgaggcaagt
    20041 gccattgcgt gttcctctgc aatgcccacg tccataaact gcttgcctgc tttctttctg
    20101 acatcaggag tgaagccgaa aaccgtcgga gtagcggagg ttatagcaca cacggacttg
    20161 tcagcctgca ttttctcaag aagataattg caggtaacag ttccgtaatc ctcgccctca
    20221 gaattgtaaa gggacttgcc tgtttcaggg tcgaacggca tatgccagtg ccatgtttcc
    20281 ttatcctttt ccgcaggagc atagcccttg cccttttgtg ttacaatgtg aacggcaaca
    20341 ggctttttgc tgtcctttac gccctcaaac gcctcaataa gcgaaggaat atcgttgccg
    20401 tccttaacat aaacatagtc aagacccatt gccttgaaaa ggttacattc agccttgccc
    20461 tcggtatcac gaagaagctt gagatttctg tacataccgc cgtggttttc tgcgatagac
    20521 atatcgttat cgttcacaac gattataaag tttgtatcca tttcccctgc aaaatcgata
    20581 ccctccagag cttcgccacc gctgagagag ccgtcgccga taacagcgat aacattgccc
    20641 tcctcgccct tgagatctct agcctttgca agaccgcttg caaggctcac agaagtggaa
    20701 gtatggccaa ccgtaaaaaa gtcatgctcg ctctcctctg ggtttgtgta gcctgagata
    20761 tcatcgaact tgtcagcgtc aaggtaggac tcctttctgc ctgtgagaat tttatgtgga
    20821 tagctttgat gtgaaacgtc aaaaactatc ttatccttag gggactcaaa aacatagtgc
    20881 attgcgatag tggcttccac catgccgaaa ttagggccaa agtgtccgcc gtgcttgctg
    20941 agcctgaaaa gcaaagcctt tctcatttcc tcagccagcg ctgtcatttc atcaacagtg
    21001 agctttttaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctcct attttactta taatttttct
    21121 tagctttagc ttacttatat tgtaccacct ggagttagct ccaagtcaac aggttttaga
    21181 gatattttac attttattaa caatcgacct aacaaaaaaa gcacggtcat gtgccgtgct
    21241 tagtatattt ctcttgaaaa aaattaatgc cgtgctataa ttatagtatc accaatatca
    21301 caccggaaag gtcttgaaag catgaaatac aaagttatag acataagtga agaggattac
    21361 ggctgcgagg gcataccgga ggacagcgaa ctcatgtgca gtgttttgat cgaaagctct
    21421 gacggcacac aaaaatggtt gaaaatagct gaccgatatc tcagagaaaa cgacattgat
    21481 ataggcagtg tcatcactgc agactagcct gcctcccctg gaatatcacg cattatccat
    21541 gcaagctgtt cttctgtagg ctcttctatc tcatagccgt tgacattgtc gcccttgaaa
    21601 cagttttgat acagctccac ccatgcaaat gttgacagtt tgcaatcaga atagatttct
    21661 ttttcacctt tgtacggcag atcttcagga tagcaggtct tgataatagg atttccataa
    21721 ggggtgcagt cctcgaactt atccaaagaa gaacccacac agcacactcc gctcagtgaa
    21781 tatcttgcca caagatactt ttcaccctcc tcatcagggt tctttttaaa cgctatctgc
    21841 gccagctcga caatagcttc tgcggcgtat ataggacaag gaattaaata tccgttgtga
    21901 agttcaaaga tcttgtcctt gtaatgaaaa gtctttcgct tttcgatagt gccatattct
    21961 gcatactgaa aaatgttcag ctcagggtca tcggagtaat agtcggtgac attttcacct
    22021 gccttactgc ggaaatagcg ttgtttctgt gcttcgagct tgccccattc cacattttca
    22081 tcagagaaat aattgcgttc aaattcgtcg atatcctcag taaaaaacga agctgtgccg
    22141 cttgtgcatg atggacagtt ggcgtcaaac aaggttattt tgtatatcat atgaatttcc
    22201 tttcctagaa gcagtgccaa gcatttcggc actgctttta ttatatccta ttatgttccc
    22261 gttttaggta aacaaagcta tttttctttt ccgcagagtg ttttttaaac aaaacttagc
    22321 tgtctattca ttgaaacaaa atcaagttgt ttatcaggct cggcacctgc attgtttatt
    22381 tctttatctg tttccagata tgacctgtta ctgtattccg ctttacgcaa tttcagttgg
    22441 tagatcaagt caaagttctt tgttatagag tcctgcttga gagcagaaaa acatattgtc
    22501 tgtagcttga atttgttgac tatcttaata aaaacatcaa gcaaatgggc agatgatgtt
    22561 tcaccaaatg gattatctat cagaaaaata gatctcactt cgtcattatc gtctatggtc
    22621 tggcgcctcc ttgcatatga tacaagtgct gtatagacca caaaataaac aagaaaatgc
    22681 tcgccaccgg aataacgtga ctcccacctt tcaagcctgc tgttgcgctc atcctccaga
    22741 cttttccaca cttttatttc aacagaatta cttcctgtga tagcattaaa aagcagtctg
    22801 tctgaaaaga atgaatttac ctgcctaagc agatcatcac cttttatttc ctctgattca
    22861 aacctttttc tcagtctgat aagtgtatca tcaataacat tgctaactct gtcctgatat
    22921 cctctgtcaa gcttatctgg tatactgatc tgcattatcc ttttgttttt gccatctatt
    22981 ttaatatagg catgatctgc aatagacttg acctgttcat acaaaaactc agcgtgcttt
    23041 ttcatatgta tgattatatg gtttttgtca ttttcgatct gctgaagctg tgatgacaaa
    23101 actttgatac tatcgtcaag cagtttcctt tgctcacaaa gcnnnnnnnn nnaaagctgt
    23161 taatatatct tgaaacaatc aagataactt ttattgttgt caatatcgat aaatgaaagc
    23221 atttgagata tccactctgc attttcagca tagcagtcaa gtattttttt atactcattt
    23281 gctaaattat ttcgctttac agtgacagca tagttagaat tattcagatc attacgaagc
    23341 ttttcaatat ctatcttgtc atcgatcggc ttgatagagc cggcattgaa tatcgtatca
    23401 agatcaatga gtttttctaa cttattgaac attgaattca actcatttat ctttagattt
    23461 gccttttttt ctgcttcctc ggcgttgttc ttattctcat catttttaat ttgactcgca
    23521 taataattgc ctgttatctg agataatcca aggataacat caaagccgct tctgtgtatt
    23581 tctttctctt tattttccaa agctacttta tttttctcgt attctttctc ctcgcttttc
    23641 ttgttgtttc tacactccac aagtttttca taagtcatac gctctgcatt ttctaaatct
    23701 ttacgcttgt ctacatcaaa tgtgatatca tagtcctcta tagtcaggtt tgcaaaggac
    23761 tcctttaacc tgttttgatt ctcatttttt tctttttcag catcactgca tatacgttgc
    23821 tgttcactta cactaccttc atgcaaggtt tgaagttctt taaattctat ttcaagactg
    23881 acgatatctc cgtctatctg ttcattatct gaatatatag cgaattcagc caccttgtct
    23941 tctgatgaac ttattttagg tttg
//
LOCUS       DAWEMR010000014        23616 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_890_length_23616_cov_12.410042, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000014 DAWEMR010000000
VERSION     DAWEMR010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23616)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23616)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23616
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_890_length_23616_cov_12.410042"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         6447 a         5632 c         4917 g         6620 t
ORIGIN      
        1 atgtggggga tatggctgat atccgcactg taaaggcgct tgaggagtcc gtgaagcgta
       61 tgtgcgaact tctggaagca acaccgcaaa acgtagtctg cgatatgcac cccaaataca
      121 atacagttgc atttgccgag gggcttgacc tgcctctgaa aaaagtccag caccactatg
      181 cccatatact ttcctgcatg gcagaaaacg actatagtga aaaggtcata ggcgtatcat
      241 atgacggcac aggctttggc acagacggca ccatatgggg aggagagata cttctctgcg
      301 accgtacagg ctttgagcgt atgggaagca taaagccctt catgcaggtg ggcggcgatc
      361 tttcctctaa agagggttgg cgtattgccg caatgataac aggctcagcc tatggggaaa
      421 ggggcggaga gatctgtgaa aagcttggta tttgtacagc gaaagagtac aagcttctgt
      481 ccgtaatggc agagaaaaaa gtaaacgcag taagctccac aagcgcagga cggctttttg
      541 acgccgcttc tgcggtgctt ggcataaggc gtttttccac ctttgagggc gaagcttcca
      601 tggcgttgca gtttgcggca gaaaaattca gccgtgagcc tttcggtatt tacgagccta
      661 tggaaaagct cactgccgaa aaagagggca ggataatgct ctgcactcag gctcttatca
      721 agcgtctttg ccagggtatg caaaatggtg aggacaaaaa ccgcctttcc tatgagttcc
      781 attcacttct tgcggacatg accgcacagg cgtgcagaac cataagcgag agaacaggcg
      841 taaaggtctg tgccttgagc gggggagttt ttcagaacaa gcttctgctt aaaatggtga
      901 agtgccgcct tgaaaaaatg ggacttagag tgcttatcca cagccttgtg cctgctaatg
      961 acggtggtat agctttggga caagcgtttt atctgaatga ggaatgagga atgaggaatg
     1021 aggagtgagg aattaaggta tcgcctgcgg cgatgatttt cttattctgt aggggcgacc
     1081 ttgggtcgcc tgagactgtt gaaaaatacc aaaataacag gcgaacgctg ttcgccccta
     1141 catataaaca gcgtatttac gccaattgta ggggacggcg ccctcgacgt cccgcttttg
     1201 tcgaactttt tcaataagct ggggcgacct ttggtcgcct gtgtatctca ttttttgctc
     1261 tgtgatttcg acttggtgat atagattaat ttttttggag gtattaatat gtgtgtaggt
     1321 ttatctgcaa aggtagtaag tgtaaacgag ggcactgccg ttattgacgc aagcggtgca
     1381 aaaagagagg tatctgctga ccttatcgac gatctcgagg taggggatta tgtcatggta
     1441 cacgcagggg tggctatcgc aaaaataacc tctgacgata gcgaagaaac tgacgagata
     1501 atggagagta tccaatgaac actatagaaa atgcaaagga gataataagc tcctacaaac
     1561 gtgatgacgg caaaaagctt cgcataatgg aggtctgcgg tactcacacc cacgagattt
     1621 tcaggcttgg tataagaaag ctccttcctg agagcattga gcttatctca ggccccggct
     1681 gtcctgtgtg cgttaccccc attggcttta tcgacgaggc ttgtatgctc gctcttgaaa
     1741 aaggtgcggt gatatgcact ttcggcgacc ttataagagt tcccggtaca gaaatgagcc
     1801 ttgcaggagc aaggtcaaag ggtgctgtga taaagacagt ctattcgccc cttgacgcag
     1861 tttcatatgc cgaaagccat agagatgagc aggtggtatt tctcgccgtt ggctttgaga
     1921 ccaccacccc atcagcctgc cttgcagtgg aaaaagcaaa aaagctgggg cttgaaaact
     1981 tctctattct tggtgcaaac aagaccatgc caaatgctta taaggctttg gagggaagtg
     2041 cagacgcatt tttgtaccct ggccatgtca acgctatcac aggcacagcc gtgtgcgagg
     2101 agcttgtgaa aaagggcgtt tcaggcgttg ttacgggctt cacggccgct gaacttctta
     2161 cggctcttgc tgttaccata gaactttctc agcgtggcga gccatttttc agaaactgct
     2221 atcctagagt agtaaagccc gagggcaacc cagccgccat aaagcttatg gaaaaggtca
     2281 tgaccccctg cgacagcgag tggcgtggac ttggtattat cccaatgtca ggcatgatac
     2341 tccgtgacga atatgctgac tttgacgcac gaaagaaatt ctccctgccg aaaataacag
     2401 gcaaaccgaa cccagcctgc cgctgcggag atgttttgca gggcaagtgc aagccctcag
     2461 actgtaaagt tttcggcaag gtctgcacac cgctgcaccc tataggtgcg tgcatggtgt
     2521 caaacgaggg cgcttgctcg gcatattatc agtatggcaa tttgtaaaat aaactatccg
     2581 aaaagaggaa taattatgac agataaattt gttacccttg cccacggcgc aggaggaaag
     2641 cagacttccg agcttatcga ccgtgttttt aaggagcatt tcgctaaccc tgacctgacc
     2701 gccgacgatg cggccgtgct ttccatggaa aagggcaaga tagcattcac cacagacggc
     2761 tttatagttt ccccatacga atttgcagga ggaaatatcg gcaagctaag tatttgcggc
     2821 actgtgaacg acctttcctg catgggcgca aagcctttgt atctctcctg tgctttcgtt
     2881 atcgaggagg gctttccaat ggataagctt gaggagatag ccgccgccat ggcaaagacc
     2941 gctaaggaag caggtgtcga gatatgtgca ggtgacacaa aggtggcagg aaaaggtcag
     3001 gtagacggcg ttttcattac cactacgggc ataggcagga taatggacaa cgccaacacc
     3061 tcaggcaagc ttgctaaagc aggcgacgcc gttatagtgt caggtgatat cggcagacac
     3121 ggctgtacca tacttttagc tcgtgacgag ttcggtatag aggcagacgt aacaagcgat
     3181 tgtgcacccc tctgggcaaa cgtaaaagcc atgttcgacg ttacaaacga tatccacgtt
     3241 atccgtgacg caacaagagg cggtgtaggc actgttcttt acgagatagc aggtcaaagc
     3301 ggtgtgggaa taacccttga tgctgagaaa ataccggtgg cagacgaggt gaaaggcgtg
     3361 tgcggtatgc tgggcttaga accattgtat cttgcctgcg agggcagact tgttatattc
     3421 gcaccaaagg acaaagcaga ggatatcgtt tctgccctta gaaagtgcaa aaactcagag
     3481 aacgccgctg tcataggcga ggtcacagag gataacgcag gcagagttgt aatgaaaaca
     3541 gagataggct cacagaccct tctcccacag cctggcggag agcttctccc tagaatttgc
     3601 tgatataaaa aatattctgc ataacacgtt ggcacattgt aggggcgaac agcgttcgcc
     3661 cgcctgattt tctcgcaaac ttatgttgaa agccgcattt gggaatattg agccaaaaat
     3721 tgcgtggata tcatgagttt gtcaggcaaa caaaacgggc gaacactgtt cgcccctaca
     3781 tatatggaat ataaaaaacg gaactgttca gcttgatttg aacagctccg ttttttgtta
     3841 tctgtaagac tctatcgcct caatagtctt tgctatatcc tcgtcactgt gagcaaagga
     3901 aacgaacatt gcctcgaatt gcgacggtgc agtgtaaatg ccgctttgaa gcatatgacg
     3961 gtagtatctg ccgtagcttt ccgtgttgca agcccttgcc tcatcatagc ttttcactct
     4021 ctcgtcattg aaaaataccg tcataagcga accacagcga ttcacgttca gacccttttc
     4081 ccttatggca ttctctatca tagccgactt tctgtcaagc tcctcataga agtttggtat
     4141 gctttctatc tgcctgatag tttctattcc tgccgccact gcgcatgggt ttccagacag
     4201 cgtgcctgcc tgataaacag agcctagtgg tgcaacgcac tgcattatct caagctttcc
     4261 cccatagcag gcaaggggca ttcctccccc cactatcttg ccaagggtag tcatgtcagg
     4321 gataactccg aaatattttg aagctccccc ctctgaaagc ctgaagcctg ttataacttc
     4381 gtcaaatata agcacagtgc cgtgcttttt cgttatctct ctgagaaatt cgagaaagcc
     4441 cttttcaggc ggaacaacgc ccatgtttgc ggcgcatggt tcaacgataa gtgcggctat
     4501 ctccctgccg tattcctcaa aaagcttttc cacagaagca gtgtcattgt attttgcaag
     4561 aagcgtgtcc tttgtgcagc cctcagtcac tcctgcaccc gatgggatag cctgcgtcat
     4621 aagtcccgag cctgccttta caagcaatcc gtccgagtgg ccatgataac agccctcgaa
     4681 ttttattatc ttgtccctgc ctgtgaaacc tcttgcggct cttattgcgc tcatgacagc
     4741 ttccgtgcct gagtttacga gcctgagcat ttccatagac ggaaagtgct tttttataag
     4801 ctcagccaaa gttatctcgc ccttgtggca agcaccgaac gtaagcccct tgtcgcaggc
     4861 ggctttcaca gcatttatca caggctcaca gcagtgaccg agaatgttcg gcccccatga
     4921 gcatacataa tcaataaact cgtttccgtc ctcgtcccat atcctagacc ctttcgcatg
     4981 gtcgataaaa agcggaacag tgccaaccgc cttgcaggct ctaacagggc tgttcactcc
     5041 accgggcata aaaccgcagg cagttttgta aagctgttca gacctcgaag tgttcattgc
     5101 ttgtcctcct ttatccattt tgcggcgtca agagcatggt atgttatgat aatgtccgct
     5161 cctgctcgct ttatagcagt gagattttca agaacgatac gcttttcgtc tatccagcca
     5221 agctgtgccg ccgcttttac catagagaac tcaccgctta cgttgtatgc cgcgatcggc
     5281 agatcatatc tgtccctggc ggctctgagt atgtcaagat agcaaagagc aggcttcacc
     5341 attaccatgt cagccccctc ctcgatatcg tcctctatct cacggagagc ctcacgtccg
     5401 ttgcaaggat ccatctgata tgactttctg tcgccaaagc atggtgcaga ttctgcggcg
     5461 tcacggaacg gagaataata tcccgaagca aattttgcac tgtatgacat tataggaata
     5521 tcagtaagag cattctcgtc aagcgccgcc cttatcgccg caactctgcc gtccatcatg
     5581 tcggagggag cgataatatc agcgccggcc tttgcaaggc tcaccgccat tttgcacaga
     5641 agcggcaaag tgctgtcatt gagtatcttg tcgcccttta caactccgca gtgtccgtgg
     5701 ctcgtatatt cgcaaagaca aacgtccgca ataataagta ggtcaggata gtgttccttg
     5761 atgtatctta ttgcctgctg tgtaacgccc ttgtcattgt aagcctctgt tgcaagctcg
     5821 tcctttttct ctggtatgcc gaaaataagc aagcccgata taccgctgtc tgctatctgc
     5881 ggcaatatct gctccagatt gtcgatagaa tactgataaa ttgttggcat agagtcaaca
     5941 ggcttcatga tgttattgcc ctctgccaca aaaatcgggt agataaagtc tgccgcattt
     6001 aatcttgttt cgcaaaccat tcttctcatt gctgggtttg cccttaatct tctgaatctt
     6061 cgcattttta tcttcctctc acttatctga aagtatcttt tgtattattc cttctgctgt
     6121 gctttccgct ggaagcagag ggccatttat accgtatttt ttaagctctg ccgccgtcac
     6181 ttcgcctatg gagatacact ttgtattctc tgataagctt cctctgccgc ggaaaaagct
     6241 cctcactccg cctgcactcg caaacacagc gtagtcatca tctatcttct ctcctgtgct
     6301 gtaaacaggc tcgtaaatat gaacatcatc aaagttcagc cctttttcag ccaatggctt
     6361 aacaagctcc gctgacccct ccgaggagcg tactataaga agcctgtcct cacaagtaag
     6421 agtgccagag agcatatttc caagagccac gctgttgaat ttttcaggca ttatgtctgc
     6481 ataaagccca gccttttcaa gcttcgcccc agtgcctttg cctatgacag gtatcttcac
     6541 ttcgcttagc tttcttatgt caatacggct ttctctaagc cttttcaaaa atatctccgc
     6601 accatttgga cttgtaagag caatatggct gtaaaagccc tctgaaagcc gtgaaaaagc
     6661 ttttaccgtg acctcatcgt gcagctccct aagctcaaat ccgccacagc ggtgagcata
     6721 cgccccaagg gcattgagcg actttataag ccgccttgaa agcccctttg tggcagtgac
     6781 agccacagaa actccataca atggaggcag gtaggtaggc gaaagatcaa gcccggcggt
     6841 ttctccaact attatcacag caggtgcagg cagatcctct ctcttcacct tttcagcaat
     6901 gtcgccaaga cagcccttta cagccctctg ctcaggggta gcaccctttg atatcaccgc
     6961 cgcaggagta ttttcgccca tgccgttttc aatgagcctg cctgctatct cagggagatt
     7021 tctaagcccc ataagaaaaa taagcgttcc accgcatttt gcatagcttt caaaatgctc
     7081 agggagcagg tcgtccgccg tgtgacctgt tataacatga aagctccttg aaagtcccct
     7141 gtggctcacc ggtatacctg ctagctcagg cactgccacc gaggaagtca cccccggtat
     7201 aaggctgtat ggtatgcctg cggctttgag tgcagttatc tcctcgcccc ctctgccgaa
     7261 cacaaaaggg tcgccgcctt ttagcctgag aacaacaccg ccctctgcgg ctttttccac
     7321 aagaagcgtg tttatctcct cctgcgtgga gctgtgtctg cctgcacgct tgccaacgca
     7381 tatcttttgt gcattttcct tgcaaaagtc aagcaaacgg ctgtctataa ggctgtcata
     7441 tatcaccacg tcacagcttt tcagagcctc ataacctctc attgtgataa ggtcataatc
     7501 tccacagcct gcccctgcaa ctataactct gccgctcata gcttatcagt cagctcctta
     7561 acaagttcaa atcttttttc tatctctttt tcgccctgag caaaatgccc cttgccgtct
     7621 gaaacagtga gagatatctt ttccccattt atctcgctgt atgcccccac aggcacagtg
     7681 cagtccgcac ccagaagtct tatcacctca cgctcggttt caaacctgag catagtttcc
     7741 ctgtgtgata cctgcgaaat tatctcagcc gccttgctct cctttgcaca ttccactgca
     7801 attatagcct gacaaggtgc aggcaaaagc tcccaaggct caaactcttt cacttcatat
     7861 tcttcaagtg aaagccccaa acgctccgtt cctgccgcac aaagtattat gccgtcataa
     7921 agcccatcag aaagtttttt taggcgtgta ccaacattgc ctcttatatc agcaaacttt
     7981 gcgttaggat aaagccttgt aaagccccca cgtctacgct gacttcctgt gccaacagtg
     8041 aaaacatcag aatcgttaaa tacaacgcct tttttggtca caagcatatc acgatagttc
     8101 ccacgagggc aaactcccga tatctccaga ccctcgccca acgtaacagg caagtccttt
     8161 gcactgtgta ctgcaaagtc tatttcgccg ctttgcaaag cctgttctat ctccgtgacg
     8221 aacacaccct tgccgcctat cttgtcaaga ggtctgtcaa gctgtctgtc gccctttgtg
     8281 gtgaaggtga ctatctcggt atcacactca ggaaataccg cctttatcat gtcaagtacc
     8341 atataagtct gcgccattgc aagtggactt tttcttgtgc ctgctctcag tttcattttc
     8401 gctcctttgt catctctata agctgttcct ctgttggaag cacgtcactt tcaaggcatt
     8461 ttttcaatgc ctctgccata gcctttttcc tggcctgctc cgtggatata ttttcaaggg
     8521 catagtcacg ggcttttccc atgacattgc atatatcccc aagcctgccc tctgtctgcg
     8581 attggatcgt ctttctcaga tgagaagcaa gggtagggct ttttccgcct gttgaaatgc
     8641 caatgcacac gtccccctct gtaacaatgg cagggaagaa gaacgtacag ctctcagggt
     8701 catcaactgc gttgcatgga atgtgtcttt gcctgcaaag ctgagaaatg tgcctgttta
     8761 caccgctgtc ccctgtggca gttatcacaa atgtactgcc gtctatgtcg ctgtcctcaa
     8821 aagcacgctc gaaaagtgtt atcatgcccc tgtttgcaag ttctcttatc tcaccgcaag
     8881 cttttgatgc caccaccttt attataggcg aaaagtcaag aagcttttcg atctttctca
     8941 aagctacctg tccgccaccc actacaaggc atggcatatt ctcaatattc gcaaaaaaag
     9001 gaaagtatgg catagtcatt ctccgtattc ttttattact tctataacct tgccgaaagt
     9061 ctgtgaatca agctccgact ttagtctgaa aaacagctct ttcgccgaaa cctcgcaaag
     9121 tcttctgtca aggctctcaa actgcggaag aaaatcatgg aacataagct gcttttcaag
     9181 ctcgtcagtt tcctcgtcaa tgataagctt cgacctctcc acgctgtcaa gctttttgag
     9241 attgttttct ttggcaagct ccgtgaaaaa gtccatgtct ataagcctag cccctgcaag
     9301 cttttccacg gcagggtcaa tatctctcgg cactgcaagg tctataaaaa gcttgttttc
     9361 attatcactg agcatatccg ccgtaacagt gtagtggggg cttgaagtcg ccgaaattat
     9421 gcagccacat tcgccaatgc gctcatatct cttttcgtat ggtataagct caagctttgg
     9481 atcgtctgaa atatattcca gcgcatttct gtgggaacgt gccgtcgcaa taacagttac
     9541 gttcttgtaa gaaagaagat ttttcaagac cagcgagcct atctctccgc tcgctccaag
     9601 aagcatcacc ctgacattat ccttataatg tgccgcttcc ttagccgcaa gagtagccgt
     9661 ggaaacggaa gtctttgaaa gctccgtctc cgtctttatt ttcttcgcac aagcgatagc
     9721 cccctgaaat atcatgttca cctcagagga aagcccaatg cgctcccttg aaagatcata
     9781 tgcacggcga acttgtccaa gtatctcgtc ctcacctatg accgctgatt ctataccgca
     9841 tgatacgctg taaagccttt ttaccgccgt cttgtctgtg aatatcatga ccttgcgttt
     9901 gagctttgcg atctccactc ccctttgttc gcaaagaagc cttatgccct cctcaggtgt
     9961 tccgcaaaag taaagctccg tcctgttgca ggtgcaaata agcacaggtg aaaggtcctt
    10021 tacgtcaaaa agcagctttt cacactgagc gctgtcaaat gtgaaaaggc tgcgaaaggc
    10081 ttgtccgcaa agcttgtgat ttacgcttat gcaggtgaaa tttttcattt ttctgtgtta
    10141 tctcctgact tttcgggata aagtgcgtca tagtcaagct tggtgaatct gtgatatgtt
    10201 atgccgccca gatcgtcctg caaacagtat ctacagccgt caggctctcc gtctggaaag
    10261 tatgcaagca ggtcaaattc atcgtcgggt gcagcagtta taacagcgtt cttattgtat
    10321 tttctgaata tctcagccgc tctttcaaga gaacacccct cttttaccgc tccgataaac
    10381 tccacaagaa acgggcttgc ctccaccttt tcgtggatag ccctcacgct ctcctctgat
    10441 accgtaacag ctatcctgcc gtcctgagca agcgaagcct tgcatgaaag tatctcactt
    10501 ttgtattcgt ctatggcttt tatcatttcc tcagccgtaa gctcgccccc tgttatggcg
    10561 ttgagggagt caagaggata aaaaagcgtc accgcattag gggtaaaccc aagctttatc
    10621 tgcgactctt ttatagtatc ggttatattc tgttcaagct ttataagctc tgtcatgaaa
    10681 attctccttt gtacataatt cctataggct ttgtatgttt ttataatatc acatatttct
    10741 catgttgtca accttgtttc ctcgcctata tattttttca gtgcattgat ataaaattcc
    10801 gtcagtggat tagaaagtac ctttttgtga gtaactatgc caatgcgcat ggacccctcc
    10861 gcttcaaggg gaacagccac gatatcactg ccgttgagct tttcgtcaat aacaccgctg
    10921 caaatggtgt agccgttaag accgataaga agattaaaaa gggtcgcacg gtcacggaca
    10981 cggatattct ttggtcgttt tatggtgctg agtatctcct ccgagtagta gaaagagttg
    11041 tattccccct gctcaaagga taaataaggg taggggagca ggtcgtcaag ggtcacagac
    11101 tttttctgcg cgaggggatt tgcagagccg acaaaaacgt gtggctttgc agaaaaaagc
    11161 tccgtgaatt taagctcttt ggactttatc tcctttgaaa gcacaggctc gttgaaatcg
    11221 ttgagataga gtatgccaag ctcgcttttc attctcgcca cgtcctcgat tatctcgtgg
    11281 gtctgcgtct cacgaatact gaaatcatat tcatctccgc caaactcttt tataaggtca
    11341 acaaaagcgt tcaccgcaaa ggagtaatgc tgagtggata tgcaaaactg ccttttgccg
    11401 tgttgctttc cgcagtattt ttcttcaagc aaactcgcct gttcaagcac ctgccttgca
    11461 tagcccaaaa agacttcacc ttcttttgat accgttatgc ccttgtttgt gcgtatgaat
    11521 atagtgatat tcatttcctt ttcaagctca cggatagcgt tagtaagact aggctgagaa
    11581 ataaaaagcg actgtgccgc ctcgctgaca gtacctttct ccgccgtagc caccacatat
    11641 ttaagctgct gtaaggtcat atccccaccc cttttcagat atatccttat tatatagcgt
    11701 atttatgaaa ttttcaagtg gagataaact attgctgtcc tattcttcca tatcttctgt
    11761 gctgataaag acacttttgc caaacagctt ttttctgatg tgctttttta cctctttgtc
    11821 actatcgtaa acgtccgtaa tgtagatgta ttggtcgctg acatcgtatg caagctgaaa
    11881 gctagggtta gggtcaacgt ttacgaaagc ttctttttct tcctttttgg tgaactttct
    11941 ttttggataa agctttctgc atttttcttt tgaacagctc actcttgcag cttcaatgat
    12001 gtaataattg cttttctcag gataatacac tgtcagctct gcacagaaga aaaacgcatt
    12061 tgcgcttatc tttatacatt cgtttggaac agctatctct ttcagcttgt gacaatcgta
    12121 aaaagcatag ccccttattt ctctaagact aggtggcaag atcatatccg taagacattt
    12181 acactgatga aaagcatggc ttccaatgac ctttagtctt tcaggtaatt gaacgtttac
    12241 aaggctttcg cagttttcaa aagtatagtc atttatcact tccacatttg caggaataat
    12301 tgtactttga agcttttcac agccgcaaaa acacccctct cccataaaag tcaagcccca
    12361 agggagcgtt atcgatatta atgacacatt ctcataaaat gcgtattttc ctatctcgca
    12421 caggcatctt ggcataacaa cattttctag cctgcggcat ttgtaaaaag catagtcggc
    12481 tatcttcagc gttatgggta gtatctcgac ctgctcccgt tctttaggac agcttataag
    12541 aacagtgcgt tctctgttgt aaagaactcc gtcagcagaa taatatctgt cacttttcgc
    12601 tgaagccgtt attccttttg aaatatagtt gtatttgaaa aagccctttt ctataaactg
    12661 aatgttttcc gaaatataca ggctcctcag ctttctgtca ccgaatgtgt ttacgcttat
    12721 atccgtcacc gccgtgccgt tgatgaacgc aggcagtgag acgtcagtct ccgcaccatg
    12781 atatttcata agctttatcg ttccattctt tgtaaatatg tattcccact ctctgctttt
    12841 caatgtgatc agacctttcc ttttattctt atcacattat acaaatatca gtgggtataa
    12901 aattggacat gaaaaagcgc ataacgtaca cactgcaacg ttatgcgctt atgtttctat
    12961 ctcatcatct ctatatcctc ataaagcccc tctatatcag ctttaagccg tcttatctca
    13021 tgaagacgat gtcggtattt ttcaccgatc tctctctgct cctccatagt cggcaaaggt
    13081 atctcacact ctttcaaccc ctttgtgtgc aatatcctta gtacctttcc gctgcgacag
    13141 ccgtcaagca tagcctttcc ctctgccgat gaaagataag ccgcaatata gtaagggtca
    13201 gccttctcct tgtcaagtcc tatccttata agcgtgggtg aaacaagaag ctttctgtcc
    13261 cccatatcgc ctgcacatac caccttgaaa ttcttgtccg tggtggacag tatcacatct
    13321 ccgtccttta tggcatattt ctcaaaacgc ttgctgtcat aaactatctc aatgcagttt
    13381 tccgcaataa cgccgtcgtc aatgtccaca agccttagat attcgcatgg cttagtaaac
    13441 tcattgtccg aaatgtagct ctccgccata agtccatgac ttatctcttt tatgatatct
    13501 ccaaaattaa ctgtatttac attcatttta gtcacctctc ttgatttcca tacatctcag
    13561 acaatgccga gatctcttct tttacaatat ccacagcctt ctttggcgag gtcagcttca
    13621 cagcttttct gttagctatt gcccagcgta cagccgtctg gatatttgcc ctgaacgtca
    13681 cgtcatagat atcgtcatta cctatgacct tattgcaggt gaactcattc ccgaatgtat
    13741 caagcacaag acttatgtct ttctttttta tgttgatatg tacacgctcc acagtcccgt
    13801 caagcatata cctgttaaga tttttgtatt gcacaggctt tagatccctc ggaacattgc
    13861 taagcgtctt tacatctctc atagagccgt cctcttcgca gatactcgcc tttaggatct
    13921 tgtctatcct gaaatgacga agatcatcat aacgaacatc acttgcaagg agatagtaaa
    13981 agccgtctgc cataacgatg ccataagggc tgagcataac acgttgaagc tttataccgc
    14041 tctgtgggtc gctcttagtg aggagcttgc cgttcttgtc ataatcgctt atggtaacat
    14101 caagcttcct gttggtgttc atagcctctt tgatattttc tatcaccaaa aaaatgctct
    14161 tgttgccata gtgaaaggtc tctctgagat tggcttcctt gctctttgtg ttacccgtag
    14221 ataagatgtt tatctttttt atcagatccc tcacatagtc cgcagggagc attggcgagt
    14281 atatgatgct gtctgccaga aactcatatt cctcagcggt gatgtcagag gcgtagtaaa
    14341 tatctgtgat gtatttttta ttgcttttat cattatcatc gttgtcattg tctgcgatga
    14401 aaacattgca gctctcatct cctattatgc tcttcaaatg ctgagccacc gtcttgcggt
    14461 taggctttat gccaagcttt tcctcaagat aaccgcagat ctgctgctga gtaagggggt
    14521 gcttttcgtc actgtgttct ttgagtatct caatgatctt gtgcgaaagc atatctgttt
    14581 ttgtatatga catttttgcc ctcctgttct gtgatatctt tctttcaata tatcactttt
    14641 gctttatttt gtcaattgtg tgccgctaag tttttgtatc ataaaaggat acaccacctg
    14701 ctttagcacc ctaacaggca gcctgtgccc gaaatccgcc gtcagcatat tttcctcacc
    14761 gaatttctcc ctgaacatat cggcgttgtg aagcagctcg tcattcctgc cgaaaatgcc
    14821 gaaagtttct tttgaaaaat cgtatgacga tgagttgtat cctttgagaa gaatttcaag
    14881 ctcatcaatg aagctccggt caatggtgta tgatacagcg ccgtcctgcc ttggtctgag
    14941 aaaatcatgc tccccaagcc cgatagcgtt ttcaatgtct gtgggagcgt tcatggcggg
    15001 attgataaga aattttggta tgccctttat aagcattgca taaaagccac ccaaagatgt
    15061 gccgacaaca aaatcaaact cattgccggc gaccagcttg cttataaaag caaatgcttc
    15121 aagtgggcga acaggtatgt caggagaaat gacctcgatg tcagaaaaaa gctttttcag
    15181 taccgatgcc gtcgctccgc cccctgcaga gccgatcccg tgcaagtgca gtatcttcct
    15241 cattagtcat caccttcgct gagatattct tctctgttct cttcgctctc aaattccaca
    15301 tcattagggt cgccctcaaa tttaagcaca ggcgagccgt atagtcccca gaaagcgtca
    15361 gtgccgatct tttttacaga cttcggtatc acaataggtg tttcaagatt atgacacata
    15421 aagaaagcct gcgagccgat ttcctccaca ccctcgtgaa gaataagttc ttccatgcca
    15481 aatatctcac tgaaagcact gccacatatt tttttgatgt tccctggtat ctccactttt
    15541 ttaagtgtcg attccccaaa agcaccattg ctgatctccg tcatggtcga ggggaacttg
    15601 acgctcgtta aaggcatcat attaaaagcc agaggctcga tgtcctctat tccctcaggt
    15661 atctccacaa taagcggagc gtacctgtcc ttgctgttga gaatgtagtc tgcataagcc
    15721 tttgaagcca tatcaaaagt aaaagtcttt tccatatccg ttacctccat atcattaaca
    15781 atagaccgac ttccacatca gaccctctgg tctctcgtga aattcaagct ttccgtcctt
    15841 gtaagagaaa tagtctgaaa cgttcttgtc aaaaacaaaa tcaggctcag cctcaagtat
    15901 tatcttgtaa gtgccgtcat cttccttgtc gataccgcca tagaacgcac ccttttcaat
    15961 gacctttacc gactttggta taacaacgcc gcttgaatag caatggaaaa atgcgtcctc
    16021 tccgataaat tcaagcttgt cattgaacct gatgttcttt atatattcac agcgccagaa
    16081 cgcacccttg ccgatagact tgagttcatg aggacaaata aggttgtgaa acggtgtgtt
    16141 cctgaacgca taatcgccta tctctctcag ggtcgacggc agacttatgt tcataacaaa
    16201 aagattgtca aaagcatagt catctatcct ctctatgccc tcagggactt caatgtgaaa
    16261 cctctccgca tatctgttcc ctctgagtat ctgatctgcc atagccctcg ccgccatttc
    16321 tgcggtgtat atcttgctct tatccattgt atcaagtcct ttctttttgt gttgctctta
    16381 tcataccacc caccatgggt aaaatttcgg atattaaaaa cttccgctat gtccacattt
    16441 ttgaacattc cgggtgctat aatgcagata cagacgaaaa acaagaatga gaaaagacca
    16501 cggaggcgga tttttatgcg ttgttacaga gcaagatgta tactcaaaga gtctattatc
    16561 agaagaacaa gagatagtga agaagagtac gatacattct ttgatgagaa aaaccagctt
    16621 ataaacgagt tcaatgaaat ggcacgcacc atgtccaaca gaagctgtgc ctttatatca
    16681 agtgccgtaa aagatcaggc gaccataggg gtaatgatga cagacattga agccgacgtg
    16741 aaagcctatg taaagaactt cttttcattt ataaagcttg agtgcgtgga tataagcttc
    16801 gaggaaataa cagtggaaac gttcaaaaga atgatcccca acggctatca cgataatttc
    16861 aagttcgatg aagacctgct gaatgactat gaactggacg acctgttcag taactataga
    16921 agatcgtaca taagcgaaaa tatcacaaag tccattgata aaaaagacgc tctcgccgta
    16981 gcaaaaaaat atctctgctc agaaaccttt gagcctgagc ttaaaagaat tttcagaaag
    17041 aacagcgtcg gtgtgacaaa aggcataccg gtacattatt tgatccaaag cgataatgcc
    17101 cataggcttg ccgataccct tgtaagcgcc ctgcattcaa ataacagact aggctctgca
    17161 agatataccg tcctcgacct ttataattta tcagcccgaa gctcagatat ccgtgaactg
    17221 ttccgtgttt gcagcggctc taccatcata atgaattgtt gtggtttaaa gtgtaccaac
    17281 agcgataatt gtacagccga tgaaactatc cccgatatcc tctttgaact catcaatgcc
    17341 tacaaaaacg agatacagtt tatttttacc ttttcaaatg acggaccttc tctccctgac
    17401 gctgtggaag aaaagcttgg cgctatcaca ttcgtgaagc ttgcggacga tactgtagaa
    17461 aacgaagatg cacataaata tctcagaaag ctttgcagag aagagaatac ctcctgcgat
    17521 aaagacctcc tttccctgtg cgcaaaggat accccatata gagcaagcga gcttatgggt
    17581 atgtacgata cttggtattc aaatgtcctg aaaacaaaaa tctatcccca gtattcaaac
    17641 ttcaaaaagc tttatgacag ggaagaagcc aaggagataa aaggctctgc ctacagcgag
    17701 cttcaggagc ttatcggact tgattccgcc aagagcgtta tcgaaaaggc gataacatat
    17761 tcaaaggcgc agaaaatttt tgccgataag ggcatgaaag aaagccgaac agccatgcat
    17821 atggtgttca caggcaaccc aggcacagca aagaccacag ttgcaaggct gtttgcaaga
    17881 atattcaaag aaaatggcgt gctttccaaa ggcgacctta tcgagtgcgg acgttccgac
    17941 cttgttggaa aatatgttgg ctggacagcc gttcaggtga agaatatgtt caagaaagct
    18001 aaaggctcag tgctttttat cgacgaagcc tattccctct gcgatgaccg ctcaggctct
    18061 tatggcgacg aggccataaa cactatcgtg caggaaatgg agaacaaccg tgacgatatg
    18121 gtagttattt tcgcaggcta tccaaaggaa atgaacgact tcctcgaccg caaccctggt
    18181 ctgagatcaa ggatagcgtt ccatgtcaat tttggggact ataccgccga ggagcttctt
    18241 agtatcgtcc atcttatggc taaaaacaat gaacataagc ttgccgacga cgttgacgaa
    18301 acgctcctgc cttttcttga aagggtctgc aagaagccga atttcggcaa cggcagattt
    18361 gcaagaaatc ttatagaggg agcattgttc ggtcaggcac tcaggctcac aagacagaat
    18421 ttcagtacca tgacaatgga cgatatcgtg accctaaaag cccgtgactt tgacctcacc
    18481 gaggacgata cccttgaaga agagccaaag catattggat ttaacagata aagaaaggat
    18541 aatatttatg acaaaagttt ttacacagga tatggcaaaa agaatggtag cttatgaaat
    18601 aaagactccc tttggctcga gttctcttgg acttgctata ccggagggct tcacaaggat
    18661 agaaaagggc gctttcgaaa atatggatat cgacactgtg atattcccta agtcccttga
    18721 agttatcgag gataacgcct ttaaaaactg cagactgaga gaggtgcgtt ttcctgaaca
    18781 ggtcagatat ataggcaaca gtgcctttga ggattgtgta tccctccaca atgtcgagtt
    18841 tgagtatgac cttatagaat atataggcga tcgtgctttc tataactgca taagtcttac
    18901 tgacattaac agcacataca gtatcgaaat accgaggagc gtaaaatata tcggcagaga
    18961 cgcttttgca cgttcagaga gcttttatga aaacgaacac cttgacttga ctatactctc
    19021 aaaccctgat ataaagatcg actgctgcta ctcccacgag cctatagacc tttctgcctt
    19081 tgccacgaag ccaacagcac aacacttcgc agagtatggc gtaataagtg ccaatgagca
    19141 gtatggcggc gaaagatatg tttttactgg atataatgcc aaagagcttt acgcagatta
    19201 tatccttaac cgaaaagacc catatgaaga ttttattgca agaatacccg agggcgttac
    19261 gtttatatgt gataatgctt ttcagaatat gcctgttgac gaggtgatat taccaaattc
    19321 tcttcaagct atcggcgagg atagcttctc aaacactaat atttcagaaa tagttatccc
    19381 accgaacgtt gagcgtatcg accgttttgc atttgcccgt aatgaatttc ttgaaaaagt
    19441 taccatgaac agcgacaaaa tatccaaaat ggatgcgtgg tttgacgatt gtccgagcct
    19501 tgaaagcata acagtttccc cttgtatcag agagatcaca agctatacat ttgacaactg
    19561 catcaattta aaaaatatcg agataagcga gggcgttgag aaaatagagt ggaatgcctt
    19621 tgacaactgc aaaagcctta aaaagctcgt tctccctacc accataaaga cccttgaaaa
    19681 tggctggggc agaggactta atacaggcaa aacaggagaa gtgaccgaaa taatattcaa
    19741 aggccacgag gattggaaga taccaaaggg ttatcatatc aaaagctata aggagggcaa
    19801 gcttgatata ggcaagtgtt ttcctgaggg aggcaacaac cctgcaaaaa taaaatattt
    19861 caccaccgaa gaaatgcttg aagaaataga agagctttat aacagagctg taaggtatga
    19921 ggaaggcaac cccgacagaa aatatgatga tgaaatgaac agatattggc atatagcagt
    19981 tttttataag gatatatatg tttcaagcga ccccgcaggc gaccatgtag gcgaatctta
    20041 tcaccatttc accattggcg gaaacacccg aaaggagctt gaatatattc tttcccaccg
    20101 agagggcata ctcttttcgt tcatagaaac atacctgcct gatgtggata tgtactgtat
    20161 ttactgtgac gatgaggagt tcgattgcga tcaggacgat gtagcctata atatagagga
    20221 cgctttcttt atgagaaaat acgcccccgg ccttaaagaa cttccaatga accttatcac
    20281 cagacttaga ctatgtgagg agcgtaaaag atgatatatg tagttggcga cctccacgga
    20341 gatctttcaa aaataaaaaa gctccatgag gataagaagc ttagtaaaag cgatacagtt
    20401 atcctcctcg gagataccgg actatcaagc acatatgaaa tgttttccac aagccttaaa
    20461 aaacagcttg cggctatccc cacaaatttt ctctgcgtcc gaggagaatt tgacgcactg
    20521 aattacacaa cctatacaga atcagagcgt tggcatcttc atataacaga gggcaagata
    20581 cacgataaca gctataaaca gaaaatatgg aactgtggca aaggtgatta tgaagaagcc
    20641 tatcccaata tcctctatgc caatgacggc gagatatttg ttatcgagga taaagctatc
    20701 ctcaccatag gcggagcttg tatgcagaat aaacagtggc ggtcagatat ctttggcata
    20761 gagtttatcc ccgataagcc tcaaaaagtg gtcaaagata aagcaaaatt caatctcgaa
    20821 cagtatggct ggaaagtgga ctatgttgtg acccatacct gcccctatga gtatagacca
    20881 acataccgct atatccccga tctgaacacc gccaagtgct tcaaacggca ttatgataac
    20941 gaaaagtttc tttccaaaat aagaaataag cttgtctatg aaatgtggct ctgcggacat
    21001 ttccatttag atcgtactgt gggcaacgtg agatttctct atgacgatat ctatgagata
    21061 aaataaagcg caggcactat ggagcatacg ctctgtagtg cctgcttccg tcatttttga
    21121 ccctcacaaa atcaagctca tcaagcccga acagccttgt aagctccgtc aacatctcct
    21181 cactgttcag cgcaggatta agatatatca cagccttgcc gccctttatc tccactctgc
    21241 cacgaggata ataattgtac tctttcccat gtgttacgtt tttcggcagt ccctcccaag
    21301 tcagcctgtg attgaaatta tcccccgact ttgagttaag tccactgccg tcaccaataa
    21361 catttcccga tttgtcgcac tctaacttct ttgtatataa ttcaccctcg ataagccaga
    21421 atattccctt gtatatcata ttattcacct caatatgata ataccgtttc ctatgtccaa
    21481 aattgcacac atagcctgtg ctatgattat atcagacaaa ggaggtgaaa gttatggcac
    21541 aatacaatgt acagttttcc attgacggcc gcagaacgca ggagatcgtg tcagcataca
    21601 acaccaatga tgcgagaaaa ataatcgaag cacgatacac cggcgcaaag atcgttatat
    21661 ggagtgtaac aaagctctga tttgttcggt cgaccagggg cagagtgtaa aacactccgc
    21721 ccttgcttga ccgaataacg aaaggaagtg tctatggtaa catcaaaatc agttgaaaag
    21781 aacaacgaag aatggtacaa caaaaccgtg cagatatttg aacgctcgta ccgcacagac
    21841 gcaagctttg tgatatctca cataaagtct gctcagaaaa agttcacctg ccaaggctat
    21901 cttatcacag ccacaaaagg gaagcccctc aaagtctttt cgggcgagag tgaagccata
    21961 gaaaagcagt tgcaaaacag caagatacca tattcacagc ttatcacagt gcaaaataac
    22021 aggtccttgc aaactgccta tatcgcctat accaaggata aacaaaagct cacagaagcc
    22081 ctgaacaaca gcggcttcac cgccatagaa gatgtggaca gctttatgtc agactatgac
    22141 aaagtaatga taagagttgc cgaaaacatc tcacagcgtc atttgttcga cagcatagga
    22201 ctatggtagg gaggtgacag catcgtgacg atcccccgcg agagaagccc accagatttg
    22261 gtgtgcttga ctaaaaaaac gccccgccct tgactttcat tttatgagta cggctgtctt
    22321 tatattctga ttttaatgac aaagcttttt aaaggtatcc aattaatagc attgttaaca
    22381 aagcagaaat caaataactt ttattcttcc ggcttatata cttttactaa attgtaagaa
    22441 tcatcaaaca aagttattct gccacatttt tcgcaatgcc aaacattata gcgaggatag
    22501 ggaatgtcca aaacgtgtat gtattcgttt ttctgtatct cacaccattc tctgtctgaa
    22561 taaacataat attccgtatc gttagggtca agctgatttg taagcatatt tccacatcga
    22621 cagtatactc tcatattcat ttctcctatg cactatttat gttatatatt tactgtgatt
    22681 tcaaaatgac ggataatgta tatcatcttc aagaacacct ctgccgacaa ctttattttc
    22741 acagtataca agaaaatttg tccccgcagc atagtccttg tcaagcagtc catactttac
    22801 aaaagaaagt atccctttcg aggtgcattt atcgaccttt tcgatagtgt ataaagctgc
    22861 ggctatcatc ggcgaaattg cgatatcatt tttatctaca aaacacagta ttatgacagt
    22921 atcttctatg atccaaaaat tagggcgttt gactttcttc tgatcccata ctattttata
    22981 ttttgctctt ttttcgggaa catagtgctt gctgacttta acatatgggt ggtcgaaact
    23041 tcttatagta cggataccta tatgtgaaga aactgtattt tcatatttaa tagctggcag
    23101 tgcgaccaaa gatgcattgc ataaggcatt taaccagaat tgcctgctgt ctttttttag
    23161 tttgatctcg tgtccgcact ttgaacaaaa atccgtcata aaacgaagtt ctcttctttt
    23221 gaagtttatt ggaattattt ctccacacca tggacatctt ggtgcaaaca tatcaacacc
    23281 cccacattag ttaatatgca tagcgcatat taattaattg atctccttta taccaaaaaa
    23341 tcattttttc ataacacata tttgaagttg aaggattgcc ctgctttcca ggtacttcaa
    23401 ccttcgtaaa ctcaacatcg acttcttcat tgtcatcaag gccgtcatca tccgtgtcag
    23461 ctttcatata attgggttta atgggattgt agcctgaatc aacaagtaat ccggacattt
    23521 cctcaacatc tgaaaaacaa tcgctgtgct atctgatttc tcctaagtgt ttgacaaata
    23581 gctattccat ttattcatat tactgatttt agaatc
//
LOCUS       DAWEMR010000015        23346 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_904_length_23346_cov_11.021339, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000015 DAWEMR010000000
VERSION     DAWEMR010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23346)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23346)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23346
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_904_length_23346_cov_11.021339"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15888..15897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16389..16398
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21227..21236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6317 a         5450 c         4775 g         6774 t
ORIGIN      
        1 tcatagccta acttcaaaat tcaaagggca gaattttttg ttctaccctt tgcttaaaaa
       61 tcacttacta aaaacgtagt aaaaactctt tcagatatat attataatat tgcgtggtga
      121 aagacgtact actttatata aaaggagcaa attattatga cgaacaaaat tgaaaatcta
      181 tgtgatatac tcacacctca agaaatctca gagttcttag gtatttccta tagctcatcc
      241 ctcaaactta taaaatataa tatggtctat ttaaaactag gctctagata cagagtcaca
      301 aaggagaact tcctcaaatt tctacaaaca tcaacaccgt cacaggcaac aatcaataaa
      361 taacaaacat aaataaagac tattccgacc taagcaaaat cattttgctt gggtcgtttt
      421 caattataat gaggtgacaa tcaatgagta taagaacaag aagaaacaaa gaaggaaaaa
      481 taactggcta tcaagcaata gttgagagag gtgagagttc cacaggcaaa agactaagag
      541 acaccaaaac attcaagacg aagaaagaag ccgaagccta catcaatcag acaaagagcc
      601 acatactcaa cggcacatac atagagccta ataaaatgac tgtctcacag gcacttgacg
      661 agtggctcga aacacaggtc aaacctacac tagcccctgc aacagtaaaa tcgtatcaat
      721 ataatatcaa aaatcacatt cgtccagcat tgggaaatat tcagctccaa aagctcacag
      781 caatgcagat acagaacttt ttgaatgctc ttgataagga gaagaacttg tctcataggt
      841 ctatgaagta tatttgcgat aacctgcata gctgtttgaa atacttcact ttcaagaaaa
      901 tgttgtcaac aaacgagtgc gattttgtta aagtacctaa aaagaagcaa aagcctataa
      961 gcagtttcta ttccatagat gagataaaaa cacttatgca ggctgttcag aatgatagat
     1021 taagaccagt tgtctatcta ggcttaatgt cactcagacg gtctgaaatg tgtgctttga
     1081 cttggagcga tattgacttt caaaataatg tgatctcggt gaataaaaat cttgtctaca
     1141 taaatggcga gtatataatc ggagactgta aaacagaatc atcaaaaagg caaatacaag
     1201 taccacccac aataataaat ctactgggcg aatacagaag ataccaaatc aaagagagaa
     1261 tgtattgcaa agaggaatat atagacagag atctgatagt ctgcaaatac aatggcgact
     1321 atttgaatcc tgccacactt tcagcaaaat tttcacagct attaaagaaa cataatctaa
     1381 gacatattcg tctgcacgac ctaagacatt cctactgctc aatactcttg aacgacctga
     1441 atattccagt cacaatagtg tctaaaagtg caggacacgc taacacacaa gtaacgctga
     1501 acacatattc ccacgttgat tcaaaaatgc agaagaaatc tgctgacgca cttgaagaga
     1561 acgtattttc agacataaat aaaaagctag gctaataaca aaggaacagt ccacaatgaa
     1621 ctgttccttt gttctaactc ttaaaaaggc ttatgtaatt cgttcgacca ttgactattc
     1681 tgtaaatcag aacattgttc acagttgtct gaaccttata aagaatgata taatcttctg
     1741 caacaagctt tcgcagacct ttctttgaaa ggaattcatc attgataaga ggtgcaataa
     1801 aaggctgtgt agaaagattg ctgatagatt ggtctatcat cttcataata cgagtagccg
     1861 cttgtggagc gtacaattca cttgcaatgt aggaaaaaat cctatcaagg tctttccttg
     1921 cgtgggcggt gaacttaatg ctgtatatat cagtagccat attttgccct catttcagaa
     1981 aggacttcct caccgtcaaa tacattgccc tcttctacat cctgttcggc ttctgcaacg
     2041 agctgataga gatgaatttg ctcaataagt ttgtcataag cttgattgga cataactacc
     2101 atatcactat aaccattctt agtgataaaa acaggttcgt cagtactgtg acaaagctca
     2161 gatattccgc ttgtgttttt caaatctttg ataggaatga tttttggcat atatgacatc
     2221 tccttacatc ttactatggc ttaattatac cataataatt tcaaaaagtc aagttctgct
     2281 gtgcgacctg cggcgacgga ataaaatgaa ttctgaatca gaacgatcaa gacaccataa
     2341 gaacacaaaa aataattgac aggcaacatt aaataaggta aaataaatct aaataataat
     2401 aaaacatctt caaactgctg tcgagtgact ggaactcgtg tatgggttaa tagctcatcg
     2461 agggttcgaa tccctccttc tccgccaaaa agtctcgtaa acacgttgtt tgcgagactt
     2521 tttattttct aaagatatga cacgcaaaaa tacttaataa agcaaaaaaa tcggacagcc
     2581 cgaagactgt ccgattgaat gtttttgtga ttattctatg gtcgtgatgt cgcttccgaa
     2641 ccaagttgtt gatatctcag caagcttgcc gtcctgcttc atttcgtgaa ggatctcctc
     2701 aatcttgtcg caaagtgcct gatcaccaag tctgaagccg atagcgtact cctctgggtc
     2761 aaggccgtca ggaagcacct tgtaatcctt acctgatgtg gatatctcgt agtttgcaac
     2821 tactgagtca aggaacgctg cgtctaccat gttaagctcc aactgctgga ggccttcaac
     2881 atttgttgca agctctgttg ttgtcgcacc atttgctaca acgtcagatg caagaagtgt
     2941 ttcctgtgct gttgagccgt tctgtacggc aatgttcttg cctgcaagat cagcctgtga
     3001 agcaacgtcg cttgagccgt ttacaacgaa caccatttca ttcttcatgt aaggctcgct
     3061 catgagcatt actttctttc tctcatcgct gacagaaagg ccgttccaga tacaatctat
     3121 tgtgcctgca ttaaggtcct gctcctttgt atcccagttt acagagtaag tttcaagctc
     3181 tacaccaagt cttgagcaaa cttcctttgc aacgtcgata tcaaagccta cgatgttgtc
     3241 gttctcgtct gtgtagccca ttggcttgaa tgttgggtca agaccaagca caagcttgcc
     3301 tgaatcaaga actttctgaa gtgagttatc ctcgcctgtt gcagcggctg tgctgtttgt
     3361 gtctgctgct gatgaagaat cagatgtgct tccgcagcct acagccaaaa gcatcatggc
     3421 agcagcggaa agtgatgcga tagtcttaaa aatagatctt ttcattatta acttcctttc
     3481 gggtatcgtc aaaataccat ttcatacgtt ttaacacttt aatataataa catattataa
     3541 cgtcactgtc aattatcatt attgcccaaa cctttgcagg ctgtgggtgc tttttcatag
     3601 aaaatgaccg cagataggcg aaaatcagca cttaccaagc tttttggctc tttccataaa
     3661 tcttgcatac ttgcctatta ccgcttctcc ctgtgtgata cctgcatttt tcagtatctc
     3721 caaaggcttt tcgggatagt ctgctgtata ttcaagttcc aactcatagt ctgtcaagcc
     3781 gagatattcg cttttgtcaa ggcatatctc aacatggtcg aaatcggtgc agagcttgcg
     3841 ctgagtatgc aaagagcctg caaggtcaga ggcagggaaa tctctcccca caagctgtga
     3901 aagctcctgc gcggtgagcg tttcaggcag gctgtcaagc tcacgctcaa actctttttt
     3961 cacatgaagt gcaccgtttt cggagatagg tgctttaagc tgtaaaaaat acttatcccc
     4021 tatttgtctt accctgatac ttgtcatgcc tgaatctctt tgtgggatat aataaaaatt
     4081 ggtctgctcg attattttgt tccactgaaa aagcttttca gcggtcttgt actgctcctg
     4141 tgtgagagaa atttttatct cacgctctat attttcgctc atgatatcct cctgaatata
     4201 ttatcatttt actacattat agcagtatca tgccctaaag tcaactggcg taaaaggttg
     4261 acaaaatccc ataaaaatag tagaatttaa gtatatgatt ttaaggagag tttgagatgt
     4321 caagaatatt gacgccacag cagaagatag agcggagcat tgtgacggat tttcgcagag
     4381 atatatggaa caagttcgtt atgggatatg agcgttacga tatgataaag ccaggggaca
     4441 aaatagcggt gtgcatatca ggcggcaagg actcaatgct tcttgctaag tgtatgcagc
     4501 atttgcagaa atatcaggga atggatatcg ggctggagtt tattgtaatg aaccccggtt
     4561 acagtgacgc caacaggcag aggataatcg acaatgcaaa gcttatggga atacccataa
     4621 agatgtttga gacgaaagtt tttgacattg catacaacac aaaacgcaac ccatgctatc
     4681 tttgtgcaag aatgagacgt ggctggcttt acaaatttgc gcaggatctt gggtgcaaca
     4741 agatagcttt gggacatcat tttgatgatg ttatagaaac catactcatg agcatggtat
     4801 acggaggaca gattcagact atgatgccga agctgcactc gaggaactac gctggcatgg
     4861 aacttatcag acccctgtac atggtgaaag aggcggacat tatccgctgg gtggagcaga
     4921 acgatctgca ttttatccgc tgtgcctgcc gtttcactga gagctacgag gggtcagaga
     4981 aaaagggtga ggtatcaaag cggcaggaaa tgaaggagct tatagcaaag ttccgtgcca
     5041 ccaaccccaa catcgaaaac aatattttca agagcgttta taacgtcaat ctgcgcactg
     5101 tgatacaata cgaatacaag ggtaagaagt acaattttat ggacacatat gatgattacg
     5161 acccacgcgt tcaggcagat gacgatgatg atgagggaat agagtaaaaa agttgtgaaa
     5221 agggcttgac aaagcggcag agatagtgta taatatatta ctgtaattgg tttgctgcta
     5281 tggctcagta ggtagagcac gtccttggta aggacgaggt caccggttca agcccggtta
     5341 gcagctccag cagggacgtg tccctgcacc cagatccgct atcatttatg gtagcggatt
     5401 tttttgtcgc cgcccatggg tacgataccc tacacacaaa aaacaccgcc cgaactcctc
     5461 ggacggtgtt atatttttat ctcgtattac agatttgtat actccataag gctcttatcc
     5521 acctcacggg caacatctct gccctctctg atagcccata ctacaagtga ctgtcctctg
     5581 cgcacgtcgc ctgcggcaaa gacgttcttt acgtttgtct gatgtgtgcc gtcggctgtt
     5641 gcgatattcg ttctgccgtc tacctcaacg ccaaaagcct ttgtgagata gctttcagat
     5701 cccagaaagc ctgccgcaat aagaacaagc tcggtttcta ccttgtattc gctgccctca
     5761 acaggcttca ttatcgttct gcctgactta gggtctttct ctggggagag ctttacaagc
     5821 actgcaccct taagattgcc ctttttgtct ttgagaaact ccttgacagt tgtctgataa
     5881 actctcgggt catggccgaa cacaccgata gcttcctcct gaccataatc tgtcttgcag
     5941 accattggcc actgcggcca tgggttgttc tcggctctcg tgtcaggaag ctttggcatc
     6001 atttcaagct gcaaaacgct ctttgcgcca tgtcttacgc ttgtaccaac gcagtcattt
     6061 cctgtatctc cgccgcctat taccatgacg ttcttgccct ttgcggaaat gtggttttta
     6121 tccgtaagac cgccgttcag cagcgcctta gttgtggatt tcagaaaatc cactgcaaaa
     6181 tagataccat ttgcatctct gccctcggct ttgatatctc taggatttga agcaccacaa
     6241 caaagtatta ctctgtcgaa gttcttgaga agctcagccg ctgtgatatc cttgccaaca
     6301 ttaacgtctg tgcgaaactc gatgccttct tctttcatta tatttatctt gcgctcgatt
     6361 atctgctttt caagcttcat gttcggtatg ccgtacataa gcagaccgcc cactctgtcc
     6421 tcacgctcga aaactgttac actgtgacct ctctgattga gctggtcagc ggcagcaaga
     6481 cctgacgggc ctgagcctat aacagcgata gtctttcctg ttcttacctt tggcactctt
     6541 gctgaagcat atccgcaggc ataggcattt tcaataatgc cgtattcgtt agcctttgtg
     6601 gtgacagcgt cgccattagc tccgcaggtg caggcttttt cgcaaagtgc aggacaaact
     6661 cttgatgtga actcaggaaa attgttcgtc atatgaagtc tgtaatatgc ctgctcccag
     6721 ttgcccttgt atataaggtc gttccattca gggataagat tgttaagcgg acagcctgaa
     6781 aatgcgttgc ctatcttcat tcctgcctga cagaacggca caccgcagtc catacatctt
     6841 gcaccctgct tttgctgctc ctcctttgaa agaggagtgt gaaattcatt aaaattcttt
     6901 atacgttcct caacggagaa cgctgctgcg tcctcacgct tatagtccat aaaaccagtt
     6961 ggctttccca tatctcaact atacttgctt tcacggcaag tatatcctcc tttcgatttt
     7021 aaaagtacaa tatctcatgt gcgcaggctt actgcttgcc gttgatgatc tgatagaatg
     7081 cttctatctg cgcctgatca ctgctcatac ccttttcttc cattgaagct atagcagaca
     7141 tcatcttctt gtaatcgtga gggattatct tcttgaactt aggcagatat tcctcaaagt
     7201 tatcaagaat acgcttgccc ttttcagagc ctgttgcgtc aacgtgctgc tggataaggt
     7261 ctttgagctg aattatatcg tacttctcct tgacctcaga gaaaagtacc atttccttgt
     7321 tgagtcttgt gtaaaggtgt ctgtcctcgt caagaacgta agctacaccg cccgacatac
     7381 ctgccgcaaa gtttttgccc gtcttgccga taactaccac gcagccgcct gtcatatact
     7441 cacagccgtg gtcgccaacg ccctcgacaa ctgccactgc gcctgagttt cttacgcaga
     7501 aacgctcgcc tgcaacaccg tcgatgaatg ccttaccgct tgttgcgccg taaagtgcaa
     7561 cgttaccgat aatgatattg tcctcggctt tgaaagtgga atcctttgga ggataaacaa
     7621 cgagcttacc tcctgaaagt cccttgccga aataatcgtt agagtcgccc acaagctcga
     7681 gggtcaagcc gttcggtata aatgcgccga agctctgtcc gcctgagccg ttgcagacta
     7741 tcttgtatgt atcatcagca agtgtgttat aatactttct tgttatctct gaaccgaaga
     7801 tagtaccgaa tgaacggtct gtgttcttga catttactgt gatagacttc ttctcaccac
     7861 tcttaagatt tttctcgaac tccttcatga gtaccttttc atcaagagtg ttttcaagtt
     7921 tgaagtcata cttggatttc ttgttgtatt ctaccttttc tgaaacgtaa tcagagttga
     7981 tgatattaga aaggtcaact tgtgccgcag tgcctgtgat accagccttt ggcttgataa
     8041 ggtcaattct tcccacaagc tcgtcaactg ttcttacgcc aagctttgac atatattctc
     8101 taagctcgga agccacgaat atcataaagt tcattacata ttcaggcttg ccggggaact
     8161 tctttctaag ctccgggttc tgtgttgcaa caccaaatgg gcaagtatca agattacata
     8221 ctctcatcat tgcacagccg agtgttatca gcggagctgt tgcaaagccg aactcctctg
     8281 caccgagtat agcggcaata acaacatcac ggcctgtcat gagctttccg tctgtttcga
     8341 taacgacctt gtttctaaga ccgttcttga taagggtctg atgtgcttct gcaagtccaa
     8401 gctcccatgg aagtcctgca ttgtagatag agtttctcgg agccgcacct gtaccgccgt
     8461 catagcctga gataaggata acacctgcgc ctgcctttgc aacgcctgcc gcaactgtac
     8521 caacaccgca ctcagaaacg agctttacag aaattcttgc gttcttgttt gcgtttttca
     8581 ggtcatagat aagctgagcc aagtcctcga tagaatagat atcgtggtgt ggcggaggtg
     8641 agataagcga cacgccaggt gtggagtgtc ttgttttagc tatccatgga taaacctttt
     8701 tgccagggag atgaccgccc tcgccaggct ttgcgccctg tgccattttt atctgtatct
     8761 catcagcaga tgtgaggtat cttgaagtta caccgaaacg tcccgaagcc acctgcttga
     8821 tagctgaaca tctgtcctca gaagtaccct ttgtgagaag tctgtcaagg ctctcaccgc
     8881 cctcgcctga gtttgacttg ccgtgaagtc tgttcattgc gatagcaagt gcttcatgag
     8941 cttcctgcga gatagagccg taggacatag cgcccgtctt aaatcttctt acaatagagt
     9001 caacgctctc aacttcctcc agcggaacag gattttcagc aaagttgaaa tccataagac
     9061 ctctgatgtt cacaggggcc atttcctcag agataagctt tgaatacttc ttgtatgttt
     9121 catagtcgcc agtccttgca gcctgctgga gcagatatat agactgagga ttgtagagat
     9181 gctcctcctt gccgcttctg aacttgtggc tacctcttga ttcaagctct gtgtctgtgc
     9241 caagtccaag agggtcaaag gctgatgtat gaagtctgtc aacgtttttc tcgatgtcag
     9301 agatagtgat accgcctatt ctgctgacag tgcctgtgaa atacttgttt ataacatcct
     9361 gaccgatacc gatagcctcg aatatctgtg agccttgata tgactgtata gtagagatac
     9421 ccatttttga agctatcttt acgataccat ggagaacagc cgcattataa tcgtcaacag
     9481 cggcatagta atccttgtcg agaaggtcgt cagcaataag ctcgtggata gtttcctgag
     9541 caagataagg gttgatagcg gaagcaccgt agccaagcag tgttgcaaag tgatgaactt
     9601 ctctaggctc gccgctttcg aggataatag caagagatgt tctcttcttt gtaacaacaa
     9661 ggtgctgatg aagcgctgat accgcaagca gtgacgggat agcaaggtgg ttctcgtcaa
     9721 ctcccctgtc tgaaaggata aggatatttg cgccctcgct gaacgccttg tctgcttcta
     9781 tataaagtct gtttatagcc ttttcaagtc ttgtgttctt ataataaagt atagggataa
     9841 cagctacctt aaagccggga atgtgcatat tctttatttt aaggatatct gttgaggtaa
     9901 ggatagggtc gtgtatcttc attaccttgc aattttcagg cttttcttca agaatgttgc
     9961 cgtcctcacc gatatagaca gttgtggagg tgactatttc ctctctgata gcgtctatag
    10021 gcggatttgt tacctgtgca aagagctgtt tgaagtagtt gaacagtggc tgaggctctt
    10081 ttgaaagcac tgcaagcggg ctgtctgtac ccatagcggc aatagactca gagccgttct
    10141 ttgccattgg caggatagat gtcataacgt cctcataggt atagccaaaa gctttctgaa
    10201 gtctttgtct ttcctcgtag ccgtgttcct ctaccttgat gtttggtatc ttaaggtctt
    10261 taagtctgac aaggttgctg tcaagccact cgccgtaagg ctgctttgaa gcatagtatt
    10321 ccttaagctc gtcatcgtcg ataactctgc cctgaacagt atcaacaaga agcattttgc
    10381 ccgggcgaag tctttccttt tcaacgatat tgtccgacgg gataggaagt gcgccaacct
    10441 cagatgaaag gataagattg ccgtcctttg tgatataata tctcgacgga cgcagaccgt
    10501 ttctgtcaag gactgcaccc atgatatctc cgtcagagaa aaggatagaa gcaggtccgt
    10561 cccatggctc catcatagtt gcgtaatact gatagaagtc tttcttagcc tgactcatcg
    10621 tgccattgtt atcccatggc tcagggatcg ttatcatgac agccagcggc agatccattc
    10681 cactcatgac caaaaattca agcgtgttgt cgagcattgc agagtcagag ccttctgtgt
    10741 ttacaacagg tgtgacctta tcgaggtcgc ccttcaaatg ctcagacttc atcgtttcct
    10801 cacgggcgag cattttatca gcattgcctc tgatagtgtt tatctcaccg ttgtgaacga
    10861 tcattctgtt aggatgcgcc ctctgccagc ttgggtttgt gttggtagaa aatcttgaat
    10921 gtaccatagc gatagcagac tcgtagtcct tatcctgaag gtcagggaag aactttctaa
    10981 gatcctgcac aaggaacatt cctttatata ctatagttct gcttgaaagt gatacaacgt
    11041 aggtgtcctc attcgactgc tcgaacacac gtcttgcaac atatagcttg cggtcaaagt
    11101 caaggccctt tttaacgccc tttggacgct tgatgaagca ctgcattatg gcaggcatac
    11161 actctacagc tctcttgcca agcacctcag gctttgcagg aacgactctc cagccaagga
    11221 tctccagacc ctctttttcg gcgattatct caaacatctt ctttgcctga tttctcgcaa
    11281 gctcgttctg tgggaagaag aacataccaa cagcataatc tctctcctcg ccaagcgtga
    11341 tcccaagctc agcggaggcg atcttcttga agaatttatg tgagatctga agcaggatac
    11401 ctactccgtc acctgttttg ccctctgcgt ccttaccagc acgatgctca agcgtttcaa
    11461 cgatagtcag cgcatttgca acagtatcgt gactcttttt gcccttaatg ttcacaacag
    11521 cacctattcc gcagttatca tgctcgaatt tcggtgaata caggcttttt acattagtca
    11581 tatcctccat gcgttccatt tttatcaccc tttcctgcca aactgtaaaa aaattatagc
    11641 ttatcgttca gcaacgtatg ttatttcacg gctcatagcc gatttttagc tgtttttaac
    11701 aaaaaatttg caggaagaaa ttaaaatcct gcaatcctgc ataagtacaa ataaactcaa
    11761 aacgtggttt ttcgcacaaa tgcaagaaaa tagccttttg ccgtcttttt tgacggctta
    11821 atcattataa cacacattac agcaaaatgc aagagcgaat gataaaactt gcataaaatc
    11881 attattaatt tttcgttaat cggtatacat atataaccta tgatttcata ttgctttcct
    11941 gggtaagcct taaaaactaa cgtaacaacg ctcttttcag cttatttaca gccattttca
    12001 gcggagaatc gtactctttg taaaaatgct gataatcctt agaattgttc cagtcgccac
    12061 cccatttcca gccgtgacgc ctgaaaatgc aatagcaaag gtctgttctg tcaatcttgt
    12121 gatcaaaagc cttgtcacgg tctgcatact ctgcagcatt ggcaggcatg accttataag
    12181 gatttataag agggtttatg tctatcgccc tgccgtagct gtgagccgaa atagtgttgg
    12241 taccagccac caccctatag ttgaaggcag aagaattgtt atccgccatt gacctttcat
    12301 catcagcgtc atactcgtcc acgagcctga ttttttctat ctcataaccg ccctcgaaaa
    12361 gctcatagaa gatatccaga acctcctgcg caagttttct cgccacgata agctccccag
    12421 actgtacctt atgctcaaag tcataatatc ttacacgcag ataggcaagg tcgcccagct
    12481 tcacataggg attttcctta taggaaacgc ctttgatacg ctctatcacc tcatttggaa
    12541 gctcctcagc cgtaaagccc tctctgtata atatcttttc cataaaaagc actccttaca
    12601 aaaagcggtc aaggcatgat accttgaccg tccttattat ttatccgcag acttgtagta
    12661 cataacaaca ggcgtaccgt aaggcacgtt gtcataaaca tatttggcgt catcatagct
    12721 catatttata cagccgtgag agccgttgta tttgtatatc tcaccgccga aatatccgcc
    12781 tcgccacgtt gaatcgtgaa gacctattcc gcagagagaa acgttgttcc agaagttgca
    12841 ctttgtatcc cactcgtcgc cgtcggcatt tgtgtctttc atacgcttgt tggtctcctt
    12901 tgaccacagc ttataaacac cagggagagt tgttctcgcc tcagatgtgg tctgacctga
    12961 aactatatag gtatcgagct ttttcttgcc cttttcataa taccacaagt gctggtcggt
    13021 aaggtctatc tcaatataag tgtcgcctat atcatcgtcc ttgcttctcg cagactcaac
    13081 gcctgtaaag tcaaaataac cggtactgta atagataggg tctaccttgt caacgctctc
    13141 gcccttttca agcatatcca caagatcgtc acaagtcttt tcctgatcta tccaccagcc
    13201 gtatttagca tcagagcttg tcggcacgat tatgtcgccc tgcaaagtgg cgtggaattt
    13261 tctcttagta ttatatgtat catacttctt tgcgagcttt tccacatact ccatggcttt
    13321 ctttctatcc acagtgtagc ttccgtcatc tttgagcttt atgatcttca taagctcctc
    13381 gcccgtgaga gtttctgtgg tgtagtcaaa atcgtatgtg acagaaagct ggaaaacatc
    13441 atttcgtttc ttgcaatcgt cctcaaggtc tgcgctcttc acctctggaa gagaataaca
    13501 acctgtgtcc ttgccaagct caacgctaag ctcacctgcc gccacgcttt gcgtaacata
    13561 agcttcaagc tttttcatat cggttatctt gtttccctgc acctcagggg tgataacata
    13621 agccgtctta tcctcgttaa gaccaagctt tgcatcagca gtttcaacat cgccccagtt
    13681 tgctttctcc acaagcttct gaagcttctc aacatcaaat gtgatgtcct cattaaaact
    13741 atagatggag ttctgtataa atcccgagaa ccatgttttt ctgttcacgc tgtcatagat
    13801 cttctgaaga tcgtccttgc cgcttagtct gtaatcaaaa tcagccgtct taaaaacaac
    13861 aggcgtgccg tcgatagttt tgaccgtaag ctcataaggc atatcgttca ccccgagcag
    13921 atcgcaagcc tgttcaaatg tctttccgcc cacgtcaatg ccattcactg ttgtgccgct
    13981 cagaaatctg ttgctatagt aagctcttcc aatgaaatat cctccgccca caactattac
    14041 gatagccgct gccgcagtgc cgcagataat gccgatattg ttgcgttttt tctttctttt
    14101 cttattcttt gacgaacctt tgacagagct gtttttcttc actgatgtcg caggcttttt
    14161 cgttatcttt gtggaatttt ccgtcatcaa ttatcctcct gtacaaaatg acgttcggca
    14221 tttgccgaca atgtatcaag aataattata tcatgctttt ttccttttgt ctataggata
    14281 ggctgaaaaa tttgtcacaa aaagagtgcc ggatattctt atttaatgta caaataaacg
    14341 tactgaaaaa aactacacgc aatcattaaa aaaatatcgg gcagataact ccacccgata
    14401 caatatcaat cttttatata cttgcccttg acctcgctgt aggtcttgca gaagcggtca
    14461 tgggagatat acgctcccca gctcatgtta tcgtccacct gagggtccat aacatatacc
    14521 tgaccgttta tcgttacctc cacccacata tgatagccgt aaccgcctgc cgccatagcg
    14581 gtcttgccgt caacggtctt tgcgtcaaag cctatatagt tgagcattgc ggcgtaaacg
    14641 taagaatact ctgtgcaagt gccgtaaccg tcctcaagaa cagccttgac aggatttgcc
    14701 cagccgccgt aatcgtagac cgtgttcttt ataatataat cgtagcaagc cacagccttt
    14761 tcgtagtttg tcatatcatc tgatattatc ttattgagat atttcagaag tatcttttct
    14821 tcctttgcgc tgagcgtcgc ctttggatca agcactgcag aattaagtcg ctgtgcaggg
    14881 gtcttgccgt tgtcaacaac aggcgcagtg gttgtagttg taacaggctt tttagttgtt
    14941 gtcaccttag cggtggtggt ctttttcttg gtagtagtca ccccaggcgt tgtggtcgta
    15001 acaggggcag ttgttgtttt tgcagtggta gtagtttttg cagtagtagt tttcactgtt
    15061 gtagtctttt ttgtagcctt tgaggcaacg gtatcgctgt cagtagtgct gacaggggcg
    15121 gaagtaacag cagaaacctt tgtttcagac tttgaagcct gctgagtttt ctttgtcaca
    15181 aggcttactg atatgctaag ctttgtgtca gcggaagtca cgctctgcac tgcggtcact
    15241 gacgctggct tctgtgatat tacagaggtc gttacgctct ctgatctcaa agctgtttcg
    15301 gaagctgact ttgttgtttc gctttttgca gtttttacag cctttacagc ctcggtctga
    15361 gaatttttcg cagtttcctt gacggctgat gttgtaagaa cagttgtgat atccccgatc
    15421 tgctcagatg tttccttgtc agaaacagca gaaatgacct ttgcattttc aggtggcatg
    15481 accgcacatg gctggataac gtttttatca agtgtcgcaa aagtcacagc agatactgtc
    15541 acaactccgc aggcagaaag cacagccaca cagcgggata tccccgactt caaataaccc
    15601 aattcagaac gctcctttca aatgtgataa actacaccat attataatga ataaggttac
    15661 aaaagtcaag gggcattgta atcaaattgt aattttcaag ccgctttttc gctcgtttat
    15721 gaagtttata tgaacgttaa gttacaaccg ttgtacattt tatatcccag gaacgacaat
    15781 aagggttcta tttttatcac ttttaacaaa tttcgcaaaa cttttattaa taataccgaa
    15841 aaacggacac ctgtgttaca aaagcccata tttcccacga tattttannn nnnnnnntat
    15901 ctatccacaa aatcacccac gcaaaaaaag aacgcaggca caagccagcg ttcccttttt
    15961 gaagtatatg aagtatatat ggagaaaaaa gtatatgtgt aaatttcatc tttttgtctt
    16021 tctcttatct tgtttatata atactccgcc aatgtgacag ttttatgata gtgtgctgaa
    16081 aactaataca caaaaaataa gtgcaaaaaa acgcctgcca tatgacagac gttttctctt
    16141 agtttaagta aattactcag cctgaggtgc tcctacaggg caagtacctg cgcaagaacc
    16201 gcagtcgatg cactcgtcag cgttaatagc acacttaccg tcagcctcgg agatagctcc
    16261 tactggacat gcgtccatgc aagcgccgca gccgatgcac tcatcactaa tcttataagc
    16321 cattttcaca gacctccttg cataaattct acaagtatca tatcaaatac tatacttata
    16381 taataacann nnnnnnnnga aaaaatcaag aggttttcgg catttcataa aaatttcaca
    16441 tagattttaa gtgatttttc agttagtatt cactaatcag ctagatgctt gccaattcct
    16501 gctgtgagat aagtgcaaat cctgcttcac tcaaagcttc cagagcggct gtaacgttct
    16561 ccactctaag gataagataa gccgtgtttt ccttagtgtt tataaatgca gaatacatat
    16621 actcaacgct gatgttgtca ttgtagagag ctttcattgc ttctgaaagt ccgcctgggc
    16681 ggtcatttat ctccactgca agcacctgag tggtagtaac ggaaaagccc tctgttttca
    16741 aggctgaaac tgctttttca aaatcgtcaa cgataagtct gagaataccg aaatcctttg
    16801 tgtcagcaag ggacattgca cggatattca catcattatc cccaagtatt tttgtaacgg
    16861 cagacaatct gccaggtctg ttttcaataa aaaccgaaag ctgttttact gtcataaaat
    16921 taccccctgt cttttagaca agctttctct tgtcgataac tcttactgcc ttgccctcag
    16981 aacgtggaat gctctttggc tcaacaagtc ttatcttcgc cttgatgcca aggatattat
    17041 ggatatcgtt ggatatcttc ttttcaagat cctcgataga cttgacagta tcagagaaca
    17101 tatcgtcatt aagctcgact cttatctcga aagtatcaga attgttcacc ctgtcaacga
    17161 ctatctgata gttagggctt gcctttgata tcttaagaag cactgactct atctgcgatg
    17221 ggaacacgtt tacgccctta atgatgagca tgtcgtcgct tctgcccatt ggcttgcaca
    17281 ttttgacgag agttctgcca catgagcatt tctctcttgt aagaatacaa atatctcttg
    17341 ttctgtatct cagcagaggg aaagcttcct ttgtgatact tgtgaataca agctcgccct
    17401 ttgagccttc aggaagcacc tctcctgtgt cagggtcgat tatctctgcg ataaagttgt
    17461 cctcattgat gtgcatacca gactgtgctt cgcactcgaa agctacgcca gggccgcttg
    17521 tttctgtcag accatagata tcgtaagcct tgataccaag aagcttctct atctcgtgac
    17581 gcatttcctc cgtccaaggc tcagcaccga agataccagc tttgagtgta agttcctctg
    17641 gctttatgcc catttccttt acagtttcgc ctatataagc ggcatatgac ggagtacagc
    17701 acaggatagt agaacctaga tcctgcatga actgtatctg tctttctgtg tttcctgatg
    17761 acatcggcag agtaagacag cccaccttat gagagccgcc gttaagacct gctccgcctg
    17821 taaaaagtcc atagccatat gcaacgtggc aaacgtcgtc ttttgttccg cctgcggcaa
    17881 ctatagctct tgcacagcaa ttttcccaaa ggtcgatatc atgctgagtg tagaaagcca
    17941 caactctctt gcctgtggtt ccgcttgtgg aatgtattct cacgcactct gaaagcggct
    18001 ttgcaagcag accgtaagga taagcctcac gcagatcagc ctttgaaagg aacggcagct
    18061 tgtgcagatc ctcaataccg tggatatcat caggcgttac gcccttttct tccataagct
    18121 tcttgtagta tggcacattg tcccagacgt gcttgacctg cttgacaagt ctttcgctct
    18181 ggagcttttt catgtcctcg tgcgccatgc actcgatctc ttcgttccaa tattttccca
    18241 ttggaacaca cctcctgtta aaatggtttc ggtcatatta ctcgggtatc aactttgcac
    18301 aagtatcata cgccgtacat ttcttcaagc atcttaatgc ctgttttcgt tttgaacacc
    18361 ttgtcacgga agctttttat gctctctttg gaaaggctgt cctcgaaagc gttcacaagg
    18421 aaatctgctt ccaagagtat ctgccagtca agcccctggg cgccctcata ggtgtggtga
    18481 tgtgcgataa gaaagcacac cctttctatg atatcctcgt caaagtcaag ctctctgagc
    18541 attttccgtg caggctcagg acctaactgt tcctgaagct ttccgccgca gtggcccttg
    18601 ccgtatctct cctccgcagg ctttatgccg atatcatgca ccgctgacgc aacatcaaga
    18661 caatacatag tgcggatatc aagtccctct gccctgccga taacacgggc aaaggtatgt
    18721 acctttataa agtgctgtat acgtttcggg tcgccagcga aaaactcggt catttttatg
    18781 ataagaccgc ttatctcgtc cattatttta cctctctgcc gagagcgaac gccttcttgt
    18841 tcagttcaag gaactttgca ggaacgcact gctcaagagc cttctgccaa acgtcctcgt
    18901 caaaagccat tttagttgac acaacgccca tgagaacaac gtttgaagcc tttgcagtac
    18961 cagcctgctc cgcaagtgcc agtgcgtcaa cagcgataac gtcaacgccc tctgctttaa
    19021 gcttgcctat gatatcctca ggataaacag ccgcacctgt gataacaggc attgggtcta
    19081 tctgctgagt tgaagtgatg atatgtccgc ccttcttgag atatgaaaca tatcttgcgg
    19141 cttcaagctg ttcaaagctg ataatgatat cagcttcgcc tttttcaact acaggcgagc
    19201 atacctcgct gccgtactta acgtatgtaa caacggagcc gccacgctgg ctcattccgt
    19261 gtacctcgct gaccttgaca tcaaagccct gtgagagaag cacgtttcct attattcttg
    19321 acgcaagcag tgaaccctgt ccgcctacgc ccactatcat aattgatctt gttttcatta
    19381 tttacacctc gaaatatatg ttatcattag tcgcatatcg cacccagctt acacatttcc
    19441 ttgcatatgc cgcagcctac gcactgtgta tggtcgataa cgcacttgcc gtcatggata
    19501 gatatagcag gacagcctat cttcaggcac tgcttacagc caacgcactt atccttgttt
    19561 accttgagcg gtggattgtg ctttacatat ttaagaagtg cgcatggacg tcttgaaata
    19621 ataacgcttg gttcttcctt ggcaagctct tccttgataa cagcctctgt ttcagccatg
    19681 tgatatgggt caacgactct tacgctcttg ataccaagtg aatggcagag catttcaagg
    19741 tcaacggcat aagcagggtc acccttaagg tttttaccag ttgtagggtt ctgctgatga
    19801 cccgtcatac ctgtgataga gttatcgaga atgataacag ttgaatttgt ggagttatat
    19861 gcaacgttca caagacctgt cataccgctg tgcatgaatg tagagtcacc gataacggca
    19921 acagacttct tctcagcctc tgcgcctctt gccttgttga aaccgtgaag tccgctgata
    19981 gaagcaccca tgcagatagt tgaatccata gcacaaagcg gtgctgttgc gccgagagtg
    20041 taacagccga tatcgcctga tactataacg cccagcttat tcaggcaata gaaaagacct
    20101 ctgtgaggac atcccggaca cataacagga ggtcttacag gcacgttatc ctcaaatgtg
    20161 atatactcag tcttctcgcc aagtatcttt tcagcaacga tagtctgtga tatctcgccc
    20221 ataaggctga aaagctcctt gccgataacg ttcacgccgt tctttttgca gtgtgtttcg
    20281 ataacatcgt caagctcctc gattacatag accttttcgc actttgcggc aaagtccttt
    20341 attatctgaa ttggcattgg atttacgata ccgagtttga gatatgaagc cttgtcgccc
    20401 agtgcttcct ttgcatactg atatgagata ccgcttgtga taactcctat ctttgtatcg
    20461 ttcatctcta ccttattgag cggtgaagtt tcggcataat cagcgatagc cttcattctt
    20521 gcctcaacaa cagggtgacg gcggatagcg ttaccaggca tcataacata cttctggata
    20581 ttcttttcat atgggatgtt ctccctttcc tctctctcgc aaagctcaac ggctgactga
    20641 gagtgagaaa ctctggtgga cagtcttacg ataacaggaa catcgaactt ttcagaaagc
    20701 tcgaaagcag ccttagcaaa ctccttgcac tctcctgaat cggaaggctc tatcatcatt
    20761 gtcttagaag cgatagcatg gtgacgtgaa tcctgctcgt tctgtgacga gtgcatacca
    20821 gggtcatcgg cgacagctat taccatacct gccgttacgc ctgtgtagcc tgctgtgtaa
    20881 agagggtcag cggcaacgtt aagaccaacg tgcttcattc cgcagaagct tcttgcaccg
    20941 cctatagaag cgccaagagc cacttccatt gccacctttt cattaggcgc ccactcagca
    21001 tatacctcgt catattttgc aacagcttct gttatctcag ttgacggagt accagggtaa
    21061 gatgatacaa ccttacagcc tgcctcatac agacctcttg caaacgcctc gtttccaagc
    21121 attagttttt tcatacaata aatctttcct ttcactgcac cgaccatttt ttcgtggtcg
    21181 gtagaaataa acaataatta aaatagacat aagtaattat accacannnn nnnnnntata
    21241 ccacaaatat caccaaaatg caacagcttt tttgatttaa agcaccaata gagtgttata
    21301 aaaagtttgc ttacggcaat tttgtcagtt tgcataaaat atcaagggag cttctgtgca
    21361 aaaatttatg ccagcttaca cgttttgagc aacaaaaaat aaatcgccgc caggcgatac
    21421 cataattcct cattcctcac tcctcattcc tcattcttac ctcctcactt gtttaaaaaa
    21481 ttgtacttga aaaaagagca taaatgttgt ataataacaa ggtatactta tatttacgtt
    21541 cggaggatat acataatgaa aaaatacaca cttgacgagg tctacacagc cgatatgaca
    21601 gcgtttcaga gagaaagata taacagagtt gcagatgaat ttgaaaagct tttcggtgaa
    21661 aagccaactg ctttcttctc agctccaggc agaactgagg taggcggcaa ccacaccgac
    21721 cacaacttcg gctgtgttct tgcagcaggc gtttcactcg acgttattgc cgctgtcgct
    21781 cctgacaacg aagagaacgt tattacaata aagtccgagg gcttcccaat ggacactgtg
    21841 aacgttgatg acgacactat ctacgaggag cagaaaaaca catctgcggc acttataaga
    21901 ggtatgtccg caggctttaa gaagaatggt cacaatgccg ggggcttcaa ggcttatgct
    21961 acttcaaacg tactcaaagg ctctggactt tcttcatctg cggcttatga agtgcttatc
    22021 ggcactatcc tcaacggtct ttacaacgaa ggcgatgttt ctgctgtgga aatagctcag
    22081 atagctcagt ttgcggaaaa tgtatatttt ggcaagccgt caggtcttat ggaccaaatg
    22141 gcttcatcag taggcggatt tatcactatc gactttgctg acgaaaagca ccctgttatc
    22201 gactctgtaa agttcgattt cacaaaaagc ggtcacgcac tgtgtatcgt tgacacaaag
    22261 ggcaaccatg ccgacctcac ccctgagtat gcggctattc ctgcggaaat gaagtctgtt
    22321 gcggctttct ttggcaaaaa ggtgctgaga gagataacaa aggaacagct tcttgaaaac
    22381 atctgcgccg ttagagaaaa gtgcagtgac agagctgtgc ttagagcgct ccacttcttt
    22441 gacgataacg agagagttgg caaggaagct tccgcacttt cagaaggcga ctttgacgct
    22501 ttcctgagcc ttatcaatga atcaggagat tcatcattca aatatcttca gaacatctat
    22561 gcttcttctg ccccaaacga gcagggtctt tcacttgctc tgtatcttgc aaagcaggct
    22621 cttggcggtg agggtgcttg cagagtacac ggcggtggct ttgcaggaac tatccaagcg
    22681 ttcgtacccg aaaagaagct tgaagctttc aaggctgaaa tggagagagt attcggtgaa
    22741 ggctcatgct atgtgctgtc cataagacct gttggcggcg tgcagatcga cctttaatat
    22801 aggagttgtc atgaaaaatc catttgcagg cttgaagaaa aagcaggtga agttgcagtt
    22861 ttccacctgc ggagagataa tgatagacgg acttcccttt gctttcggag tggagagtgc
    22921 agggaaagcg tcagacaaag gactttgcgt ttcgatatcc ggtgaagctg tcaatgacgg
    22981 acgtgttacc ttttcaaacc ttgaatacta cgaaaaccat ggcggaaaga taacccttgt
    23041 gaagcatggc ttttcacttg tgaccaaaaa ggacggcaag aaaatttatc aggcgaagtt
    23101 taccaaaata cctatcaccg aaaaggacac ctcagtgctt ttcagaaagc tcaccgagga
    23161 agaggtcgtc caacgactca actgcgagat agcttttaag gtgacacctc atttcagcgg
    23221 tgaggacaca cccgaagtca tgctcacagt ttatccttat gaaaatatgc tcacagggtc
    23281 ggctgtgcag tggctaaagg tcacgtctga caaggactat tttctgcaca aatattctaa
    23341 taaata
//
LOCUS       DAWEMR010000016        23240 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_913_length_23240_cov_11.855769, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000016 DAWEMR010000000
VERSION     DAWEMR010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23240)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23240)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23240
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_913_length_23240_cov_11.855769"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5083..5092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16987..16996
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22989..22998
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5887 a         5047 c         5061 g         7215 t
ORIGIN      
        1 gccgagccac agttcttgac tttatgagct tattgtggta aacttaaaac caagtccatg
       61 tgtgaaaaaa ttccggcagt gccttgattt tatgtgcgtt gagccgaggg ttgagcttgc
      121 aagggtgttg gacgatatta acgaattttt ttagtaacag aataggcagg aaagtcctgt
      181 ctttaaaata ggaggaaaat ttatgagcga atacaagatc tactcattgc atatgggtaa
      241 ggcaaaatgt gctgttgatt tgggcgatgg ctctgagcgt ggagagtatg taaatcagga
      301 ttatattctc aacaaaatgg gcagaccaca cagaagcata aaccttatgt actgctacta
      361 tccgttggat aaggagttcc ctggaagaat ttctgaggta atgaaagaca aagatgtttc
      421 ttttgcatgg gattatccat atgatgatta tttcacatac aagggcggtc ttgagggcaa
      481 cacagagggc gagcctttta ccttcatgag agatgtaaga cgtcatggac aggacgttac
      541 actcacactc acagttgacc cacacgtttc agacgaacag cttatcgcta tcgcaaatga
      601 ccttagacct ttcggcagac ttcttcttag aataaaccat gaagctacag gcaactggtt
      661 ctcattcaac aagagatgca cttatcagga agttgctgat ttttactgcc gtttcaataa
      721 gatacttaag gaatatgcac caaacgtttc tactattctt tgtatcggtg gcattgaaga
      781 tcttaacaag aaagagatcg aaaaggaagc agagttttca caggctgtca aggagacaga
      841 catctggtct gttgacaagt acatggctct tcactggggt tggccttatg acgttgctga
      901 aagaggcggc acaagccaca gcagaggcaa ggtcaaggac atatacaata tgacaaagcg
      961 ttcttttgag agattcaagg agataaacgg aggcgttaca aagccaatgg taatgagcga
     1021 gttcaacgct gacggtgatg ttacaggtcc atacgatcag gcagcaatgg taaaggaatt
     1081 ctgcgatatg ctcaaggctg acggcgagga ctggttctca ggatttactt tctatcagtt
     1141 cagagacaga ggacgtcttg gtcttgaaat tcaggaccct aactatgctg actgcggtat
     1201 cgagcagcct gttatgaaga catacaagga aattatccac gatgatttct tcaagcctgc
     1261 aatgacaaga ggcgatgagg ttacactgcc atgcacgctt cgttggggcg gttctgagga
     1321 cgctgagggt cttgagatcc ctctccactt tgaaaagaat ccacacttct gcgaggttat
     1381 atttgatgat gattcaaaca tcatgatgga gataaacggc aagtggttct ataagtcacc
     1441 tgaggcaaag acgatcgacc ttatggcagc tttctttgag aagccgctgg agggtgaggc
     1501 tgatctgact ctcagacttt ttgctcctcc tgcaagcggt gagaatgatc tgtctattga
     1561 ggacggactt atgaacagct acacagagat caagaagctt cctaatatcc gtatcagatt
     1621 cgagccagtt gagctttaat atccaaaaat ttaagggacg gtctagccgt ccctttttgg
     1681 tttatagggt gacaaataat gcaaacggga cgtcgggggc gccgtcccct acaaaatata
     1741 ggtaaacggg cgaacagtgt tcgcccctac aatagaaaat cgtttgctgt acgaattttt
     1801 gtacaacaaa cgatttttat attgttattt atcgcatggt gcttgttttg ccattatctg
     1861 cattgcggct tttagaccgt caacggcggc ggacattatg cctcctgcat agcctgcacc
     1921 ctcgccgcag ggatagagat tttccactga gagagatacc atatctgcgt ttcgtgttat
     1981 gcgaacaggg gagcttgtac gggtttcagg tgcggtgagc attgcacctt tatcgccgaa
     2041 gcatttcatt tttcgtgaga aattctgcaa gccgccctcc agcatatcag ttacgaattt
     2101 aggaaataca tcacgaagct ctgtcgcctt acagccaagt gcgtaggtgg gtgaaactgt
     2161 gcctgctatt tcaggcttgc catcaagaaa tccgccaact gtcgtgcctg cgcctttata
     2221 tgagcctgtg agagtataag ctttttgctc tatagaccgt gcgaaagtca ctccgtcaag
     2281 cgggcagtga ccaaagtcgt caggggtaac ggccacgaca actgcagcgt ttgcgttctc
     2341 accgtcacgg gcaaactcgc tcatgccatt tgtgacagtg cctccgcttt cggaagctga
     2401 cggcacaact gtaccgcctg gacacataca aaaagtgtaa acacctctgc cgtttttatc
     2461 acgccatgaa agctgatatt caccttttgg aagcagtgga ttgcctgcat gatcaccgta
     2521 taagctttcg tcaacagccg cctgcttatg ttctattctt gcgccaacag agaacggctt
     2581 aggctccaga aagatgtttt tgcactgcaa aagctcaaaa gtatcccttg cggaatgacc
     2641 aatagcaagt ataagggcag acactttttc gttcttgtca cctgctgata tagtctgcac
     2701 tttgccgttg gcgatagaca gatcagtgag ggcagtgtca aatcttacct cgcccccaag
     2761 ctctattatc ctttttcgga tagccgtgac gatatttcgc agattgtcag tgccgatatg
     2821 cggctttgct tttgtgagga tctctttcgg cgcaccgaaa tccacgaagc gttcaagaac
     2881 atatctgcaa agagggtctt ttattcgtgt ggtgagcttg ccatcagaaa atgtgcctgc
     2941 accaccttca ccgaattgca cgttgcaatt ggtgttaagc tcgcctgtga gccagaattt
     3001 ctgtaccgat gcagtacgtt tttccattga gctgccacgc tctaaaataa tcggtctata
     3061 tccctgctcg gcaagggcaa gccctgcgaa cattcctgct ggtccaaaac ctgcaatgac
     3121 aactttgcca tctgcttttt ctgtggatat aacaggcttg taaacactgc gctcaacata
     3181 ggtcaggaaa gtgttcttct cacaaagctt cttttcaagg tcggcactgt caagtgtcac
     3241 gaaaactgag tggacaaagt gaatgtcgtt gcgctttcgt gcatcaagag agcttttgtg
     3301 tatctcgcag tctatggcgt gctttgaggc actgcctagc tttttcagag cggtggagat
     3361 aacttcctgc tctgttgcgt taagggggga gcttatttga tttactatta ttggcatata
     3421 tcctcctgcg tttttaccga aaaatcggga cgaatagatc gtcccgataa aattctatta
     3481 gtctgaaaca actatctgta tctcgttttt gccgaaaccc caaacgttgt tgtaggtcgg
     3541 tgaatagatt atagcctctc gagcataaga accagagtca attatgtcgc tgagattgac
     3601 ctcagcgtat aagtcgctgt ctttaagatt gtttatcaca tcttcaggac cgtagacctt
     3661 gacgtttgtg agcttttttg tttctatctc agtggacttg cctgaaggct tgcccgttgc
     3721 gatgatatgg tctgatgtta tggtgaactc cttagaggtg tatccctctg agtcaaagga
     3781 tacacgaacg ttttcatcgc cattcatgtt gatctcacct gagttgagag ggatcttgaa
     3841 gctgaacact tttgcaaggt ttatctctga aagttttatg gagccgatag tgagcgtttc
     3901 tgtatctgag tcgtcaagat ttggcgatac cacagatatc tcttcctcgg aaagcttcag
     3961 tggcagtgag ctgacatcaa aattatccgt acaattagag atatctacct ttagctttgc
     4021 tgtctttttc ttgtagataa caaaattcac atcgacagac tttgtatcct tgacttctat
     4081 tttagatgga tcaacaacgt tgtcgtcatc gtcatagaat acgatatcct gtgtgttgat
     4141 agtcgtgtcc tcggatattt tctttgattt tgagatctgt gccactgctt tgcttatgtt
     4201 gtcaaggtca ttcttcggac cttcaactgt tatctcgctt ggcgatgtgg tagtttcttt
     4261 aaggatatag ccctcctcag cgcttatgag cggggcttct gcggtgagtg ggatagtctt
     4321 tgtggtaagt ctgtcaaact ccacagaaac agtgtcaggg ttgacagaaa ctacagttac
     4381 cttgtcggtg gaatgtgatg acttgacgtc gatgtcaagg tcgtaggtac cctcttttgt
     4441 tacgtcatca agattgacag ttgccacaag atctgcggca gtatagctgc ctatctcata
     4501 gttcatgccc tttatctcaa cgtctacgga gatatcctta tagttgagtg cggaaagtcc
     4561 tttttctttt gcctgcgttc cgtcaagtga gaacacaaca ggaacgtttg ttatagtctt
     4621 gtttattgta ggatactgag tgatagaaag ggcaaaccat attattacag caatgattat
     4681 ggaaagtatt ctcagaccaa agtctgcacc atggcgattt tcttttttag ccttttttac
     4741 tttttccatt ttgaaatcac ccttcctgca aaagaatttt ctctaatgga attagcgtcc
     4801 ttttcaggca aaagacgatt gtccagaagc cttttcagag agtcctttga atatcctctg
     4861 gtaagctctc cgttttcggc aactgagatg ttgcctgttt cctcagatac cacgacgact
     4921 attgcgtcag agttttcgct cattccgata gctgcacggt gtcttgaacc gagttgtttg
     4981 tttataagtt cgtctttctg cggtcttggc aagaaacagc ctgccgcaag tattattccg
     5041 tctctgatta ttacagcacc atcatggagc ggtgtattcg ggnnnnnnnn nngaagaata
     5101 tgtttccgaa tatcgctgtt gacggtatgc agttaagtat cgttcctgta gctatctgtt
     5161 ccccaagctt tgtctttctc tcacagatta taagcgcacc tgttttggtg gaagaaagac
     5221 ttgcggcaga atcacatata gcgtcaatag cttttttcca atgttcggtg ttgtcgtcag
     5281 agtcacttga gctaaagaaa ttaaagccgc ccagacttgt tcgtccaacc ttttcaagaa
     5341 cacgtctgag ttcaggctga aacattatga taagagcaag gaagccccac tggaagcaga
     5401 tccttagcaa aaatctgata gtttgcaaat taaagaatac tgcaagcagg tagcaaccta
     5461 taagtataag gatacctttt gtgagctgct gcgcccttgt ttcgcgaata agctttatgc
     5521 cgtggaatat cagaaaggag aggatagcaa tatcgacaat gtcattaaga cctattgatg
     5581 taaaaacgcc ccaaatagcg tttataacat aagcgatact gtcaagcata gttcacaccc
     5641 tttcgataat ttctgtaaat gtaatgaaaa aaacaaaaca aatacagatg attttatata
     5701 tattaatagt atatcatatt ttttcgcaaa gtacaagggt tgaacaagat tttttttaat
     5761 aaaaagcgga cctttttcag tccgcttcta tatcagctct cttttggaag ataaaacctt
     5821 cctgagagtt tttgtgtttt tacttcgttt atgaaggctt tcggatcggc ttcttttaca
     5881 gcgtgtatga cctttttggt ctgtgccgct gatactactg aatagactat ctgtctttcg
     5941 cagtgttcat aagacccctc tccgtcaagt atggtcgcac catgctgact cactgcataa
     6001 atagcgtctg ctatctcttt gggcttgttt gttacgataa gcaaggtgct ttgctggaat
     6061 tttttgtaga gcatatgcaa aacggttgtt gaagcatatt gaaagatgat agaatacagt
     6121 gctttgtccc agccgaacat aaagcctgca acgcacaata tgaccgcatt gaagccaagt
     6181 ataacattaa aggtgtccat attgcttttc tctgaaagat aaatggatat aaagtccgtt
     6241 ccacctgatg ttgcaccacg attaaggcag aggctaatgg caacgccgtt tatcattcct
     6301 ccgaagatgc ttatgagaag cacgtcctgg gtgattatgt gattggggat aaggtcggtg
     6361 aaaaagctgt tgagcagtat catcacgcag gaaaaagccg tgaacttttt gccgataaat
     6421 ctgaaaccga tatacacggg gattatgttc agagttatgt ttatgatgct gaacggaagc
     6481 attatgcctg caagctgttt cactgcacgc tgtatgagta tggacattcc tgtcattccg
     6541 cctgggtaaa gaccgcctgt gtttacaaag gtctttgtgt ttagcgccat cagaaaggcg
     6601 gcgacagtca cggcgataat tgtgaaaatg tcttttctga tatctttttt catggctttg
     6661 ccactccttt catatgtcag caattattct cctttaagct cggcaaattt tttctgcatg
     6721 gtagggttat ttttggtaag actcttgata gtgagagcgt cagccttttt gtttgcaagg
     6781 cgcagattgt tttctgatga aataaggttt tcttttatct ttgtgagatg tgctatagac
     6841 ttgtctattt ctgtgatagc ttcgccaaaa cgtctgcttg caagttcata gtttcgggca
     6901 aagccctcct tgaaagtgtt gatgttttgc tcaaacattg tgatgtcgat attctcgttg
     6961 cgcacctttt cgagctcttc cttataggaa agggtattca tggcggcatt tctcagtatg
     7021 gtgattattg gtataaaaca ctgaggtctg actacataca ttttatcata agcatatgaa
     7081 acatctgtga tacctgcgtt ataaagctcg ctgtcgcttt ccagaagtga aacaagtaca
     7141 gcgtattcac attttttctc acgtctgtcc ttgtcaagct ctttgaagaa atgctcgttc
     7201 tttttcttgg tggaggtggt atcctgctcg tttttcattt caaacattat ggatattatc
     7261 tccacaccgt tttcatcaca ttcacggtag ataaagtcgc ccttgcttcc tgagcttgcg
     7321 tcgttatcct tgtcaaaata agcgtttctg aatgcggtag ctcttatctt gttgaactcg
     7381 ttcatgcagt gctgttcaag gctctcgccc accatttttg tagacagacg gctttttagg
     7441 tctttgtagt atgcgatagc ttcgtccttg ctttttatct gtgcattaag ctctgcaata
     7501 gtggaatttt tctcactttc cacttttgaa agtgcagtgg taagctccaa agccttttca
     7561 gtatccttat ttttgatatc tgtttcaaga cgtgttatct cggctttata ttcattttct
     7621 gacttttgca tttgcagagt atgctcagaa ttgagctgct gtatctgcat atcacgctga
     7681 gatatttcct gcctgagctt gtccttttcg ctgttgaatt gcgatcttgc tttctccaca
     7741 gccattgata tggcaagttc tttttctgtg gtcatagatt tggtctgagc ggtaagctgt
     7801 gcgataagct tttcgttttg acttatctgc tcctttagct gctcaagcat tttttcatat
     7861 tcggctcttg tgctttcctg agcctttgtg acagcaagtg tcttttcact ctcaccgtta
     7921 gccgcagccg ctttcagctt ttgtatctcc atatccttat cattgacagc ctttgtatac
     7981 ttgctgtcaa aatcaagctt agcaagcttc aaagccttgt ctttttcgtc agccacacgc
     8041 ttgtcaagct ctttatcaaa ctcagccgtt tttacctgtg ccacgatagc cgcatagccc
     8101 tgttcatcaa ctttgaatac ctctccgcag tggggacatt ttatttcaga cattttatgt
     8161 acctccgatt tcttattttt tctttaatat gattatacca tattttattc tcctatacaa
     8221 gaaatgaaga agattttttt tacgacaaaa aagcaggaac gtaaaaatca cgctcctgcc
     8281 aatttattaa aaagccaaaa gttagagagc ggcacaaagg tcgcccatac ataaaatagt
     8341 tacgtcattt gtaggggacg gcgccctcga cgtcccgctt tctcggtttt tcagcaccct
     8401 cagcaggaaa gtaaaaaatc acgctcctgc gaaaatttag ccaagtattc tctttatatc
     8461 ttcttcaggt gtgctgatag gagtaagctg gaacttatcc accaaaacgt caagcacgcc
     8521 ctttgaaacg aatgcaggga gggtcggtcc gaggtagata ttctttatgc ccaatgaaag
     8581 cagggtgaga agtatgcaaa ctgccttttg ctcataccat gaaagcacaa gtgtcagcgg
     8641 cagatcgttc acgccgcagt cgaacgcctc ggcaagtgca atggctatct ttatggctga
     8701 gtatgcgtca ttgcactgtc cgcagtcaag tatccttggt atgccgtcga tagtgccaag
     8761 atccatatcg tttatgcggt attttccgca ggcaagagtg aggataaggc tgtcaggcgg
     8821 tgtgagcctt gcgaagtcgg aatagtaact tctgctcggt gacgcaccgt cacagccgcc
     8881 tatgaggaag aagtgcttta tcctgccctc tttcacaagg cttattatct tttcggcatt
     8941 tgacagcact gcgttgtgtg caaagcctgt tgttacagtg tgaccgccgt tcatgcctgt
     9001 catttccttg tcctcatcat agccgccgca ctcaatggct ttttctataa caggcgtaaa
     9061 atccttgtcg tcgccaatgt gcacaagctc aggatatgac acgatagagg tggtgaaaac
     9121 tctgtcgcag tagctttgac ggacaggcat tatgcagttt gttgtgaaaa gtatcggtgc
     9181 agggatattg tgaaattccg tctgctgatt ttgccagcct gtgccgaaat tgccctttag
     9241 ctgtggatag gctttgagct ttggataacc atgggcaggg agcatttcac tgtgagtgta
     9301 gatgttcacg cccttatctt tggtctgctc aaggagaagt ttcatatcgt gcatatcatg
     9361 accgctcacg acgataaaag gccctttttc aatggtcagt ggaacttctg tagggacagg
     9421 gtcgccatat gcgtcagtgt ttgcggcgtc gagaagtgcc atacatttca ggttcacatt
     9481 gcctgtttca agcaccagcc ccaacaggtt ttcagggtcg ttctcactgc ctatggcacg
     9541 cagagccttg tagaaaaagc ttgtcacctc gctgtcagtt ttgccaagtg cctgtgcgtg
     9601 ataagcatac gccgccatac cctttattcc gaagagtata agtgatttca gcgagcggat
     9661 atcctcatga tcgttccaca gttttttcat gtcgtagtca tcaaacttct gagagttgat
     9721 gcggtttcgc tcaatgtgta tctcctctgt gagctgtttt atggtgacat tattgaagtt
     9781 cacgttcgtg atagtcgtaa aaagcccttt gagtatgagc atatctgttt tttcagttga
     9841 cgagccattt tctgcggcgt ttgcaagggc gataagtgcg ccgataagct cgtcttgata
     9901 aactgctgtg tcggcttttt tgccgcatac gcctactttt ccgtcacagc ctgtgttgtg
     9961 tgcagtctgc tgacactgga aacaaaacat atctttcact ggtaagacct ccgtatattt
    10021 gtgataggtc tattatgtac cgatatgtga aatttattcc tgcgttttat gagaaaaaca
    10081 gcctaacagc cagtgcagag aatacaaatc ccataaggtc tgaaaggcac gctgcagcaa
    10141 atactgaggg ataagctttt tgctttatgg ctgaaaaata tactgctatg gtgtagaatg
    10201 tcgtttccgt cgagcccatt aaaacgcttg ccacacgtcc gctgtagctg tcaggggaaa
    10261 cgcttgaaag aatggtttca aggcaggcga gtgcgccgct ccctgaaatg ggtctgacta
    10321 tggcaagggg tatgcactct tctggaaagc ctagaaaagt ggtcacaggt cggagcagtt
    10381 ttgctataat atcagccgca cctgatgatg tgaacattcc cactgctgtc ataagcagaa
    10441 tgagcattgg tatcagcccc acggcgattt tgaggttttc agtcgcaccc tcgcagaaca
    10501 cattcactat atccaccttt ttcacaagcc catagcaaag tataaaaacc ataagcgcag
    10561 gcacgaaaat gtcaccgaaa ttcatggcgt tcgtcctttc tgaggttgag ggcatagacc
    10621 ataagacagc ctgcggtcac ggagataagg gaggagaaaa tgatagcagg cgttacgtca
    10681 aagggggata atgcaccgtg ttcaaggcgc attgccgcaa tggtggaggg gataagctga
    10741 atagaagccg tgttgagcac cacaagcata gaaatgcact tctttcgtgc cgcgggcgga
    10801 agcttctgca tggctttcac tgtggctatg cctgagggtg tggcggcatt gcctaatcct
    10861 aaaaggtttg cagtcaggtt cattgtcacg gcattcatga cctcggggca gtgtgcggca
    10921 tctctgaata gtagccttgt gacaggggat agcatacggg caatgaccgc tgttatgcca
    10981 gccttttcac atattttcat aagtccgccc caaagacaca tcgagccact caggtatatg
    11041 ataagctcca ccgctttgtt acatccttca agagaagcca ctgatactgc tgtcatgtcg
    11101 cctttaagaa aaccgcataa caaggcggat agggacaata tcacaattat tacgcttaat
    11161 ataattaaca ctcctttcag ctagtattca tatcaagtta atatataaaa aactttgaca
    11221 ataagtgtga aattttggtg ataacgcttg acaaatgtaa ttatgtttgt tataatatgt
    11281 ttatcataag atttatgtta aatgaaatac tatttttgtg tttcaaatta atttaaaggg
    11341 gattgatttt atgaaatcta caggaattgt aagaaaagtt gacgagcttg gaagaatagt
    11401 tctacctatc gagctcagaa gaacctacga ccttgctgtt aaggacgcta tcgagatcta
    11461 tacagatgat gacaagatca ttctgaaaaa gttccagaga acttgtatct tctgcggaag
    11521 cggtgaagag tgcgttgaat acaagggcaa gacaatttgt gcagcttgta tggcagagct
    11581 caagggtgaa gctaagtaat tacatatttg tagtaatatt atgaaacaga caggacggac
    11641 atgaaagtgt ttgtcctgtt tttattttat gtgatagaaa aagcgtgtat gccaatgaca
    11701 tacacgcttt ttttacaata ttagtttttt gaaatcatct tcgctgccat taaataccgt
    11761 catgtggaga tattcgccgc tttcatagaa tgagaatttt acacggttgg tataagtcca
    11821 aaacgtccaa ttcatatcct cgtcaggctt tgaaaacagg ctctcatacc atatcttgtc
    11881 cttgttaaat ctgctgtctg aagcaacgat gtttttgtac gtccgttctg tgcagtatat
    11941 gacaggtctt acgccgtatt gcttttctat cctgtcgcag aagtctgcaa ggttatctgc
    12001 ggcttcgtaa tagtcagggg gcagaagtct gtaaagtcct ctcagtctta cctccactgc
    12061 agggataagc ctgccgctta gatcatcgcc cacagcattt atgaaatgct ctgcctgctt
    12121 agtgccgtca gtatcaagct caagcacatg atatgctccc actgatatat cagtgtcctt
    12181 tgagccgttc cagttgtcac ggaacgcacc gtcctcaaag gtagtgcctt tggtggctct
    12241 tatatatgcg gtctggatat tctgcttagc aaaactcttc cagcgtatct cgcccattga
    12301 ctctgttaca acaggtcctc ttattggata gtcacgcttt ttcggctcat tgaaccacag
    12361 tctgcctgta tacaaaagca ccgcaaggtt tatcacaagc acaactgcac acagtattat
    12421 cagccgtttt acgagctttt tcagaaatct gtgacgcggc ttttcttcct cgtagacctc
    12481 gtaatttgtg tagtttgaaa ggtcacgatc gtcatactcg ctgtctattt tgtttattgc
    12541 ttccttgtca agcttgaatt tcttgctgcg tttcattagc agtatcctct cggttaaatt
    12601 cctcagaatt tgcgataagc tccgcaaagg tatatgtgtc aagcttgttc cttaccattt
    12661 cagatatctc gttaaaagct ttctgataac agcagatacc gtctgcacct ctgttgcagg
    12721 tgcagtctgg ggaaagacat cttgaaaaat aatatgtacc ctctatggct tcaagaacgt
    12781 ctttcaaggt aagctgtgca gggtctttat ttatgatata gcctccctgc gtgcctttat
    12841 atgacttcac tatatcagcc gcaacaagct ttctcagtat cttcagcgca aatcgaagtg
    12901 taacgcaggc atcattggaa atggtctttg cgtccatttt gtctctttcg cggcatagaa
    12961 cggatactat tcttactgcg taatccgcct caagtgttat aaacatttat ttcctccgaa
    13021 acttagtcaa gataatctct taccttgttg ctcctgttag ggtgacggag ctttctgaga
    13081 gcctttgcct ctatctgacg tattctctca cgggtaacgt tgaactgctg acctacttct
    13141 tcaagagttc ttgaacgtcc gtcctcaaga ccgaaacgca gtctgagcac cttctcctct
    13201 ctctctgtaa gagtggaaag cacctgattt atctgctctt taagaagcac aagtgaagcg
    13261 gcttctgccg gagccggagc gtcatcatca gggataaagt cgccaagatg tgagtcctcc
    13321 tcctcaccga taggtgtttc aagagaaaca gggtcctgag cgatacgcat tatctctctt
    13381 actctttcaa cgctcatatc aagctctttt gctatttcct caggtgacgg atcatgaccg
    13441 ttcttgtgga gaagctgtga ggatactttc tttacctttg tgatagtttc taccatgtgt
    13501 acaggtattc tgatagttct tgcctgatcg gcgatcgctc ttgttatagc ctgtcttatc
    13561 caccatgtgg catatgtgga gaatttgaag ccctttgtat agtcgaattt ttcaacggct
    13621 ttgataagac ccatgttgcc ttcctggata aggtcaagga agctcattcc tctgcccaca
    13681 tatttctttg cgatagaaac aacaagtctg aggtttgctt ctgcaagtct ttttcttgct
    13741 tttttagcgt ccttttcatt gccgctagac atcttttctg caagttgtat ctcctcctcg
    13801 gaggtaagaa gcggcactct gcctatttcc ttaaggtaga tcttaaccgg gtcgtcgata
    13861 gcgatcccgt cagttgaaag ggaaatatca acgccgtcct gatccagatc gtcttctggg
    13921 tttaccatgt caaggctgtc gtcggtgatg atatcatcaa ttatctcaat attatttgcg
    13981 gcacagctat catagaagct gtccatttgc tcaacgtcat agtcgagctt ttcaagagcg
    14041 tcagttatct cttttgtagt gagcttgcct gtagttttgc cgtgttccat aaggttatca
    14101 agtatctgtt tcttgtctgt catttcaaac actccaatct caatttttac atcggttgat
    14161 ctttgctaat tctttttgcg catttcttca aactttttga agaagtcatc gtcggatata
    14221 tcctcctgtt tctccttcgg cttgtagttt aaaagcacat ttatataatc ctcagcgacc
    14281 ttggcgtcaa tgcccagttc gctgtatttt tccagtatct ccgttatctt acccacttcc
    14341 tcgggtgaaa attcaccatt aaaagatgat acagagcaat cctgcaaatc ggttatcttg
    14401 ctcgtcaggc tttcgtaaac acgcctattg aagctggtaa cgaatttgtc gggcggaagc
    14461 ctttttagaa catctccgca ggcgtcatga ttattgtaga gataatatat gatacccgct
    14521 tcagcggcgg attctcgtct gtgttcattg gcttcggggt ttatggtgtc acgcctgtca
    14581 gcgaaggtta tagtgcgagt ccattccttt ttgctatact gatagcgacg gtttttaagt
    14641 atagcattaa gttctgcatt gacagttgtg atgctcacat tctgttcagc cgccaccttt
    14701 tttgcgtata tctcacgctc agtaggggag cttatatccg caaggacttt ataggctttt
    14761 ttgagataat ctatcctgcc tatatcagtg tccatatcaa tgccgtcttt gcatttgtct
    14821 agctcaaact ctattgcacc gtctgatttt ttcagcaaat ggcgaaagtg gtctgctccg
    14881 aatttgttta tgtactcatc agggtctttt gcaccgttca tttttataac tgtggtctta
    14941 aggccagttt cacctagaat ttttattgct ctcgttgttg ctttctgacc tgcgccatct
    15001 gcgtcatagc atatgacaac gttgtctgca tagttggata ttatcttcgc ctgctcaggg
    15061 gtgagcgcag ttccgcagga agctacggca ttttcaaagc ctgcctgatt aagggaaata
    15121 acgtcaagat taccctcgca cagaagaatg gtcttatctt tcactgcggc gtttttggcg
    15181 aagttcatgg agaaaagaaa tctgtttttt gaatacacaa gggtatcttt tgagttaagg
    15241 tattttctct tatcgtcagg tgtgagcgct cttccgccaa agcccactat atgacctgca
    15301 aggtcgataa aagggaacat tgcacgattt acgaagaaat cgaacgtatt tcgggtattc
    15361 ttctgtgaac gtgagataag cgatccttgc tccaattctt gctcggtgaa gccctcagag
    15421 agcatatagc ttttcaaggc agaccaactg tttggagcaa agcccattcc atatttcttt
    15481 atggtttcaa tactgagctt acgtttgttt ataagatagt caagacattc cttgccctgt
    15541 ggtgtcttta gctgatgata gaaaaacctt gcggctgttt tattcatttc atagaagcgc
    15601 tttctggtat cggtgcgctt gctgtcatag ccgttatcct ccggaagcgg cactccgcca
    15661 cgttctgcaa ggagcttgac agcttcccaa tagtccagat tattgtactt cattgtaaaa
    15721 gtgataacat ctccgcctgc tccgcagcca aagcagtgaa aatactcttt gtcagggtga
    15781 atatggcagg acggggtttt ttcgttgtga aaagggcaga ggcaaacata atctctgcct
    15841 gttcttttga gggtgacgta gctgctcatg acctctgcga tagggttggc agctttaagt
    15901 ctttctataa aatctatagg cagcggcaaa tttcttcctc cttgggtatg tgctaatact
    15961 ttgtccaccc caatgggata aacagatttt taaaggtctc aatggcaaag ccgtctgtca
    16021 tgcctgatat gtaatcgcag atagcacggt caattccgaa ctgataggcg agattcatat
    16081 aaaaggcagg cagcttttct atatgatgaa catagtagtc atagagcttt gctatcatgt
    16141 ctttagcctt gacttcttct gatttgcact ttggatttgt gtacacaaag tcaaacataa
    16201 atctatgcag tgcagtgtag gcgtcactca cttcgtcgtc catatgcagg ttttcagctc
    16261 cgcccttgat aagggaggtc acaagagtgg ttatcctctc agacttggat ccgccaagaa
    16321 cctttgtgat gtttttcggg atatcctcct cggtcataat gcctgcacga acggaatcgt
    16381 cgatatcgtg atttatatag gcgataacgt ctgcaaatcg cacaacatag ccctctttcg
    16441 tgtcggctat catgttagtg tgctttaata ttccgtcacg gacttcttct gtgaggtcaa
    16501 gacccttacc gtctttttcc aaaacatcca ctacacgcag gctttgctca tagtggtgaa
    16561 agccgtcagg gctaagctcg ttgagagccc tttctcctgc atgaccaaaa ggcgtatgac
    16621 caaggtcatg acccagtgcg atagcttcgg tgagatcttc gttgagccgc aaagcccgtg
    16681 agatagtccg tgcgatctga cttacctcaa gggtatgagt aagtcgtgtt ctataatggt
    16741 cgcctgcagg ggacagatag acctgagttt tatgcttcag ccgtctgaaa gagttgcagt
    16801 gaagtatcct gtcacggtca cgctggaaac acgtcctcag cggacaaggt tcgcaaggct
    16861 ttttacgccc ttttgaatca acagacaagc aggcgttttc gctcagaaat tgtttttcaa
    16921 acaactcggt ttgttctctc ggcagcacaa aaattccccc ttacagtata taaaacatac
    16981 taactcnnnn nnnnnntcaa aaaagccttt atttttacat aaaggctgaa tgtttttgta
    17041 taatcgcaca aatctcatgc aaaatcggca agaagatttg tttcttctga caattcaagc
    17101 ttgccgcagg atatatccgt aagctgttct ttcagcttgt ctgaaaattc agacttcaca
    17161 agaaacgcaa gatctacaga atcggcaaag tcctcttttt ccattatcac gccatattca
    17221 ggcaacgcat agctgacctt gccataaagt gtatagtcgc aggaaaagga tattcttgaa
    17281 cactcacaca ttttcatttt tcttgcagcg ttcactgcca ttgaacaacc ctgagaatag
    17341 gctcttgtaa gaccgcttgc ccccaaaagt attccaccga aatatcgtgt caccaccaca
    17401 caaacgtccg taagcccaga tttgtttatt acatcaagca caggcactcc gcctgtaccc
    17461 tgtggctcgc cgtcgtcgct gtatcgtgaa acgttaccct ctctcaacac ataggcatag
    17521 acgttgtgcc tagccttgcg gttttcggct tttatcctgt ttataaatgc gacagcctca
    17581 tcagcggtgc tgacaggtgc gatatgcccg ataaattcgg actttttctc cgtaaagctc
    17641 gcctgagctt cctcatagat tgttgtatat cccatttggt ttcctcatat aaaagaaaaa
    17701 aggaacggaa ttatccgttc cctttgaaaa ttagtcaaga ttaaatctct tcttgaatct
    17761 gtcaacacgt ccgcctgtat ctacgagctt ctgcttacct gtgtagaatg ggtggcactt
    17821 agaacaaatt tcaaccttga tatccttctt tgtagaacga gtatggataa cattaccgca
    17881 agcgcaagta attgttgttt cctcatactt tgggtgaatt ccttctctca tttgacttcc
    17941 tccttccaaa ctaaaattat gactcataga agcccatcat ttcagtttag acagtagttc
    18001 tatgtagtat ttatcaatat gctgaacaaa tcagctttat tattatacca ttatatacgt
    18061 cacttgtcaa gcacttaaca ctttttttac atttatcctg ctcatttcga caaaacaaaa
    18121 aggcaaccca tcggctgccc tttgcttgtg taaaaatttt ggagaggatg aaacgagcta
    18181 agagtttgat cttagtgttt acattctttt tctttctctg ttctacatta catattatag
    18241 cctttagggg ctataacttc aagaacagac aagttacatt tcactacaat actgttacaa
    18301 attatttcaa tttgtaactt taaaataacc ttaaattttt ctcaaagcag gcttgttatt
    18361 cgtggtcaac tccgtatgct gcacggtatt ccagcatttt gtcaagatat tctttgccct
    18421 caacaacacc gtcctgaata gccttgatga tatcgttgat gtttacgata gagtatacct
    18481 ttacgccaaa gtctttataa gcctgttcaa cagctgaaag gtcggtgtca agagcctttt
    18541 ccattctgtc aactgttatc accatgcctg ttacgtttac gtctgcggca cttttgagct
    18601 ttggcattga ctctctgaga gccttgccag atgtcataac atcgtcgatg attattacct
    18661 tttctccgtc tgcaagcttc tttccaacga acattccgcc ctcgccgtga tccttagctt
    18721 cctttctgtc aaagcagtaa gaaacgtcaa tgccatactt tgttgcaagc gcgataacgg
    18781 ctgaaacagc aagagggata cccttatatg caggtccgaa aagtgtttca acaggtattt
    18841 tgttgtcagc tatgcaatca gcgtaaaagc tgcccagctt tgcaagctgt tcgcctgttt
    18901 tatagtttcc gcagttgatg aaatacggag cttttctgcc acttttaaga gtgaagtcgc
    18961 caaatgtgag aactccgctg tctaccataa attttataaa ttcttccttg taggtcatat
    19021 tgaacttcct ttcaattatg ttactttgat tataccacat ttttcacggc tttgcaaggt
    19081 ttttttgtct gccgaacata agtccgtgag agcctgtggc aaggcagaaa acgccgtgtt
    19141 ctcgcatatc tccacatatt gagtgtataa cgccgtattg ctgtctatcg gtatttatac
    19201 gtgcgaagct ttcgcccaga tcatcagaaa tataaaagcc gtattcgcca ctgatatttc
    19261 ctgtgacata cattgcagga gtttcttcaa gcccttttcc aaagcctatg caattaatcc
    19321 tgtcctcatg gagaatatca cttataccca cgctgtcact ctttatctcg aagcggtaaa
    19381 gtccctttat gccgtttgca agccagaata ctccgctttt atctggctgt cttgaaagct
    19441 gatagtgagc gtatcgtgga cactttatgt ccgtttttat ggacacttct ctgaaagttg
    19501 cacctttgtc acggctgatg taaagcccca gattttctct ttctgctatg gcgaagaaaa
    19561 gatcgctgtt gtaatagtcc gagaaaattt ttacgttgtg ttcatctgct gagtttccac
    19621 tgctgtccgt aaaaatggac ttttgccatg ttttgccctc atcgtcggta tagcagacag
    19681 ccttgttaga aaagcctctg ccgcctgcaa cagcccacat tatgcgtttg ccgtcggcag
    19741 atatcgctgt ccaacctgag tctacattag gcttttcgat gttgtcaagc ttttggtcta
    19801 taaactcaga aagcccacgg ggcatgggaa gtctttccca cgttgaaccg caatctttgg
    19861 ataagatcag accacctttt gttttgcctg tccagttgcc acggggagtt gcgacgatgt
    19921 attcggggtc gctgagggtg agatcagcat taaggcaggt gatgtagcgg tcgccatttt
    19981 cgtttgcaaa ggaattttcg cactcattgt cataatcttc aaaggcaaag ccaccaaggt
    20041 cgccaatgat atctataatg cgattcctgc catggggagt ggtgtataca ttaaggtgaa
    20101 cagtttcctc gatgccttcg cagaatggtt tgacaagcac atctccgctt gcaagacctg
    20161 ttatcatgaa cagacctgtt cctgtgttga ataaggctgt gtcgctgtca aaaggtgaaa
    20221 gctttatgtc gcttatccag tggacacagt ttccgccgcc gttgtattgt ggtttcatgt
    20281 atgggatatt ccaatcgaag cggctatatt caagcctatt cattattatc ttccagcttt
    20341 caccgctgtc ggtgcttgta tagatcatat ctccgccatt ttttcggcag acagttgata
    20401 gcaaaagcat tttgccattg gaacatattc cgctgtagcc gcatgggata tggtcctcgg
    20461 gggtgatgtc tttgtcaaaa acagtcactc cattttcgat gcggtatctg tataccttgc
    20521 cacctgaaag cgagcctgtg tcgcaagcat aagcacccat gccaccgtag aatacttctc
    20581 cgcttgaagc aaaagtgatg tatatgtatc tgctgtcgct ggtgatacgc tgtggcacga
    20641 agccataata ggaggaattt tcatatttta cagggtcggg ggcggtgagg ggaacgaagc
    20701 ttttcaagcc gtctgtggag caatacagag tgtgacctct tgttacaccg tcaagggagt
    20761 ttgcttcacc tgaacagcct gctataaaaa gtttgccctc actgtctgtc catataagcg
    20821 agatgttttt ctcgccaaac aggtctacgc tttgccagct ttcgcccata tcctcagaat
    20881 agataattcc tgcggtctga gagccgaaat acagcctgcc gtttttgtaa ataagtctgt
    20941 cgcctgtggc tctgccaact tcgtttccgt gaacgctgca tgggagaggc ttttgtgtga
    21001 tgttttcacc cttatcagaa gatacgcaga gatagtttga gtgcttatct ccgcaggtga
    21061 aaaacagctt atttgggtcg ttttcgtcaa gggctattgc aaggggatag gtctgtgcag
    21121 ggtcgtctgc ggtaactttg tctgccagat acacccaagt ttgtttatca aagtcatagc
    21181 gataaacgcc accgatgtcg gtgcgtatat aaagcgttct gctgtcggtg gggtggaaag
    21241 tgatacctgt cacaaagccg cctccgccta ttttcagatt gccatattcg tatttgatag
    21301 gtgaaagaat tttcatatat ctctccgtta ccattcgata gtagaaacgt caacagggtc
    21361 tttgtttatc tcgaccttat ccaccttgct gtttttgacg gttataactc tgtcagccat
    21421 tggtgcgatt gcagagttgt gggtgatgac tataactgtt acaccgtcat ttcggcaggt
    21481 atcctgcaaa agtctgagga tatttttacc tgtgttgtaa tcaagagcgc ccgtcggttc
    21541 gtcgcaaaga agaagcttag gatttttcgc caatgctcgt gcgatagaaa cacgctgctg
    21601 ctcgccgcct gaaagttggg agggaaagtt gttcattctg tcctcaagtc ccactttttt
    21661 cagcacttct tcaggctttg aggcatgtgc gcatatctgt gtggcaagct ctacattttc
    21721 cttagcagta agattattta caaggttata gaattgaaaa acaaagccta tgtcatatct
    21781 gcggtatgtt gtgagctgct ttttattaaa agtggcaatg tccttgccgt caacaaggat
    21841 cttgccctcg tcacaggtat ccatgccgcc gagcatatta agtatagtag tttttcctgc
    21901 accgcttgcg cctactataa cagcaaattc gcctttttct atctcaaatg aaacaccgtc
    21961 agcggcattt atggttatct cgcccatttt gtatctttta taaacgttct cgaattttac
    22021 aaagctcata gtctgcaccc ccatattgtc tttacgttat tgtatcacaa taatatgaac
    22081 atttcaaggg cttttgctac ttgaaatgat ggaagtgata tgttataatt gtatctgaaa
    22141 caggcgtgag cctcacattt tgacaaggag agatataaat ggaatttcag aagcttattg
    22201 aaagcagaag aagcataaga aaatatgacg gcagtaaaaa ggtaacaaaa gagcagatag
    22261 aaacgctcat agctgccgca acagaagcac catcgtggaa aaactctgaa actgcaaggt
    22321 attactgcgt tctcagcgac gagatgacaa agagggtaag agaggagtgc ttgcctggat
    22381 ttaacagaga aaggtctgaa aatgcggcgc ttcttgtaac gacttttgta catacaagag
    22441 caggttttga caaagagggt gatcctgata atgaagtcgg caatggctgg ggtttttacg
    22501 atcttggtct gcacaatgaa atacttcttc tcaaagcggc tgagcttggc ttaggttcgc
    22561 ttgtaatggg catcagggac ggagaaaagc ttcgtgaggt gcttgaaata cctgaaagcg
    22621 agatcgtagt atcggttata gctgttggct atcctgcaga agcccctgcc agacctaaaa
    22681 ggagagctgt tgaggatatt gcaaagttct tttaatatgg gagttatgca gtttttagct
    22741 tgtatttgtg atattgacct tcctgcgctt tagtttggtc gtcagacctt tcttacagca
    22801 tggggaggtc tttttgtaca taaaaataag gcgttagaac aaaatctaac gccttactga
    22861 tattcttttg aacacgaagt cttatcgttg ttcacattac aagacttgtg aagtggctta
    22921 aaaccttact tcatagcctc ttcaactgca actgctgttg acacatgacg gaaatgatat
    22981 tcttaggtnn nnnnnnnnct tgcaactcgt caattctttt ttgagttatt ttattttgat
    23041 ttaataactc atttattttc tgattgtaaa cttcaaccac ttgttctggc gtatcatttt
    23101 caatagatag agccacgata ttcataaatt tattcaccgt gttcttatta gtttctatct
    23161 gcttttttaa acgtccaatt tgtttttgca aatcattgtc aatatttgca atttgttctt
    23221 ttagattttg aatttgtgaa
//
LOCUS       DAWEMR010000017        22323 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_969_length_22323_cov_12.077376, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000017 DAWEMR010000000
VERSION     DAWEMR010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 22323)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22323)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22323
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_969_length_22323_cov_12.077376"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3694..3703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6214..6313
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16128..16137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6453 a         4267 c         5323 g         6160 t
ORIGIN      
        1 caaggaactt gcggcagaaa ataacacctc ggtaatattt atcactcata accttggagt
       61 tgtggctgaa atatgtgaca gcgtttcagt aatgtatgct ggaaggatat gcgaaacggg
      121 ttctgtgctt gatatttttg ataatgcggc tcacccatac acaagggcgc ttctgaacag
      181 caatcctaaa gaggctgata caggaaagta tatggcaact atccctggag cgccgccgct
      241 tttgtataac aagttcactc attgtccgtt tgcggagaga tgtgataaat gtatggataa
      301 atgccgcaaa gaatttccaa cgctgaaaac tatcagggca ggtcacaaag catcgtgctt
      361 cctgtcggac ggaggtgaaa agaatggctg aaaatattct tgaggtaaga cacttgaaga
      421 aatggttcaa gatcggaaat cctgcgatca aaaaggtcgg tgtaagctac ctccatgcct
      481 gcgatgatgt cagctttgaa ataagaaaag gcgagatatt tggcattatc ggagagagcg
      541 gatcaggaaa atcaacgata ggaaaatgcg ttcttaatct gcagagcagc gattctggtg
      601 agataatttt cgacggacag gatattttcg gatattcgca gaagaaaatg aagcccctga
      661 gaaaggatat gcaaatggtc tttcaaaatc cgcttgcatc tttcaaccca aagaagcggc
      721 ttggaaagac gtttgcggaa tatggaaagt ttctgggcat gaccgatgaa cagtatcaga
      781 ataggatgaa ggggctttta aagtatatca agctttctga tgatgtgctt tcaagactgc
      841 caaaggaact ctctggaggt cagcttcagc gtcttgccat tgcaagggcg ctgatgtcag
      901 agccaaagtt tgtgttggct gacgagcctg tttcggcact tgatgtttct gttcaagctc
      961 agatacttaa tctgatgata gaccttaaag aaaatcttgg tcagactatg ctttttattt
     1021 cacatgactt gaatgttgtc cgtcatcttt gcgacagagt ggctgttgtg tatcttggcg
     1081 ttatcgttga aatgggtcct atagatgagg tgtattcaaa cgttatgcac ccatatacgc
     1141 aggcgcttat ttcggcaaag ccaaaggaac atccgcttca gcaaaaggag cagataatcc
     1201 ttaaaggtga catccctact gccatagacg tgcctgaggg ttgtcggttt gcttcaaggt
     1261 gcagccgtta caagcatggt ttgtgcgacc acagaacacc aaaactcaag gatatgggca
     1321 acggacactt tgttgcctgc cattatccaa aaggtcataa ctctgatgaa gaggattgat
     1381 atagaagttt atatgaaaag gagaatttat catgaaaaac tacaggaaac ttttagcagg
     1441 ttcgttggcg ttgtttctcg ctgtaggtat cacaggctgt agcaatactt caggaggcgg
     1501 aacaggtgtt gcaaatgaag acggacttgt tgttttgaat gttgcgtcaa caaatcagcc
     1561 tgtatccttt gatcctgctg acgcaaacct tgttgtaaat gaatacacag agtatgattg
     1621 ctacgatacg ctccttgatt ttaatcagga cggcacagat gttgtacctg cacttgcaga
     1681 aagctgggaa caggtggacg atcttacata tacttataag atccgtgagg gtgtaaagtt
     1741 ctccgatggc tccgatctta caatggacga tgtgttgtat tctctaaatc gtgtgcgtga
     1801 ggacgcttat ggaatgagct atctttttga gcacgtcgac agctttgagg tagatgagaa
     1861 aacaagaacg ctcactgttc accttactca gcctgacagc acatggaagt atgttcctgc
     1921 aacaacgcca tgtcagatct tgaagaagtc tgttgtggaa gctgaaggtg acgcttatgg
     1981 aacaaatgaa ggctcagtag tgggtacagg tccatataag tttgtatcat gggctgctga
     2041 ctcacagata gttcttgaaa agaatgaata ttggtggggc ggtgcagata atcttgctat
     2101 cgacaagatc aacttctatg ttatgaaaga cgccagcact atcgcattgg cagtaaagtc
     2161 aggaactatc gattttgcac ctggaattac aaatgatgtt cttccgactt atgagtcgct
     2221 gagcaattat aatatcattc acgattttga gacttctacg gtttttgtcg ccttaaacac
     2281 acaggttgcg ccgtttgatg atgttaacgc aagaaaggcg cttgcttact gtatagactc
     2341 ttcccttgtt cagcaatcca ttggcggaga gtattcggca aagcttgatg ttacttgttt
     2401 atccgataat atgtattata tggatcctga caagtggcac actgcagtgg gagaaatgga
     2461 ggattacaca gttcaggatt atgataaggc aaaggagtat cttgctaagt caaagtatcc
     2521 tgacggattt gagttcgatt tttacacttt atctgccaat gttccggaag ctgagcttat
     2581 tcaatctatg gttggtgaga tcggtatcaa aatgaatatc aaggagatac tttcagcaga
     2641 tatgttcagc tatttgtacg gctttaatga agatgaaaac ggcaacagac catatcaggc
     2701 gtttggctct agctgggtat ctgactatct cgaccctgtg ggcaatctga aaacaatgtt
     2761 ccacagctca aatacagtac cgggatgtgc aaatcaggca atgtggaaga atgcagactt
     2821 tgacgcactt attgataaat cttacgaaac aactgacgat agtaagagag tagaatactt
     2881 tattgaagct tcaaagatag ctgctgatga ttgtgcttat atcccacttt atgtgcccga
     2941 gtcggtttac gctgtcagcg ataagtttac atttactcca agtcctcagt cattctggaa
     3001 cttctcttat actgattttc aggtaaacaa atgatctgaa caattaaaat aatacacgct
     3061 gcggcattta taatactatg ccgcagtgat gttatctgaa aggattgatc ttatgattat
     3121 gtcagcggat acgatctgga ttgccatatg caccgtgctt gtgtttttta tgcagacggg
     3181 atttgcaatg cttgaaacag gctttacaag aaagaaaaat tcctgtaatg ttatcatgaa
     3241 aaacattatg gattttgcgg tcggctcgat cttttattgg atcttgggat ttggcataat
     3301 gtttggtgca acaacaggcg ttgtgggcgt gatagatctt ttctctaacg gtgattattc
     3361 ggctgcttca caaactattc cacagcctgt atttatggcg tggcagttag ttttctgcgc
     3421 aacgtctgct actatagttt caggtgctat ggcagaaaga actgctttca agtcatatct
     3481 tatttattcc gcagttatga gtgcagttgt ttatcctata agcggctgtt ggatatggaa
     3541 cgccgatggc tggcttgctc agcttggctt ccacgatttt gcgggcggaa ctgccgtaca
     3601 tctcttggga ggttctgctg cttttgcagg cgccgctgtg ctaggcgcac gaataggcaa
     3661 atatgacaaa aagaaaaagt caagggctat actnnnnnnn nnngctatac tgggtcagaa
     3721 tatacctttg gcggctcttg gagcgtttat actgtgggta agctggtttg gctttaacgg
     3781 cggctcggta gtgacttccg aaagcggatt tgaccttgca gctatcggca gtgtgtttat
     3841 gaacactata ctttcttcat cagcatgtgc cgtatctgca atgataatta catgggtaag
     3901 atacggaaaa tcagacataa cgatgacctt aaacggcatt gtggcaggtc ttgttgccgt
     3961 cacagccggt gcggatcagc ttccacacta tgcagcgctt ctcgtgggcg taggtgcagc
     4021 ttttgttatg atctatggca ttgagtttat cgaccatatc tgcaaggtgg acgaccctgt
     4081 tggcgcaata tcagttcatg gcctttgcgg agcttttggc acaataatga caggtgtatt
     4141 ttctgtagaa aagggcgtta tctatacagg tagatttaac tttcttggcg tgcagctact
     4201 tggagttttg tctgttgcgg tatacggatt agctgcaatg acgctacttt tcgttatact
     4261 taagcataca gtaggtatca gagtttctga aaagtctgag ataatgggtc ttgaccgctc
     4321 agagcatggt tggcagggca ttgtgactga tgatcttatt tcagatctgt cagacggaaa
     4381 tgccaagagt gttacacaga tagacctctc aaagcctata gatcgttcag cgtataaagc
     4441 tgacggaaag atacgcaagg tagttatact tatgaacagc tcgaaatttg agtcgctgaa
     4501 agacgcactt gacgagatag atataaccgg tatgaccgta acaaatgtca atggctgcgg
     4561 aatacaaaag ggcagcaccg attattatcg tggctcggaa gcaaagtcac atctgctccc
     4621 gaagataaag gtcgagatcg ttatcagcac agttccactt ggacttttgg tagacacggt
     4681 aaagcgtgtg ctgtacagcg gaaatatcgg tgacggaaag atctttgtat atgaggtaga
     4741 aaatgtaatt aagatcagaa ctgatgaaga gggcaaaatg gcgcttgaat agaagtttgc
     4801 tgacgaaaag gggtattcat tatgaaccag gaacaaaaaa agaaatatct ttccataaaa
     4861 gaaatgggtc aaaggcgagg tatttcttca aaaacactgc ggtattacga tagtatcggg
     4921 atcttcaagg ctgattatgt tgaccctgat acggggtaca gatactatga tccacagcaa
     4981 tatgagaagc ttggcacgat acttgaactg cgcagcctaa atttttcgct gaatgagata
     5041 aaaggatatt tcacggagag aaatatgaaa aaatctgtgg atatgctgaa agaacactat
     5101 aagcttcttg aagaggagat acgtagcaaa caaaggctgg cggagactat caaagaaaaa
     5161 atagatttca ttgataaggt tatcgacaat aatatcgagc tggagaagtt ttcttttgag
     5221 gattttccag agcgatttat catatcacag ctcaataaat ctgataaaag tggtgaatcc
     5281 gttgcacttg aatttatgca cctagaaagt catcttcacg aaactgcgcc gatcttggct
     5341 tcagaccgaa taggctttta tatggatttt gatgatattt ccgagatgga aaatatggat
     5401 aattggcaca ccatgataat gtgcggtgaa gaaatgaaga attcaagaca tttctgtgtg
     5461 ctccctgcgg gaaaatatct atgtgcatat tttaaggatt ataccgctga cagggattta
     5521 ttcataaaaa tgtttttgga atacgtcaag tttaacaaca taaaattgaa aggaataatg
     5581 atacagcaat ttaaaataga tgtaactgtt actgacgatc cgaaagagac aatcattgaa
     5641 tttcaggcac ttgtttgaat ttgtctgaaa atgttcttga tagctgtttc ggccaaatat
     5701 aaaacaaagc cttctgagca ttgtggctca gggggcattg tttccactac tttgattttg
     5761 aattccggcg tgtatctttt gttcggctgt ccttttggca taaaaatgca ccatttcgtt
     5821 agatttttta ttggtatatt tctattatac cacattgtct aacaaaaggg gagaatttca
     5881 cttatgatac ggggtgtttt tgtgtgcaaa taaaaaataa tagaggtctg acgtttgagc
     5941 cagacctcta ttttgctatg gataatcact ttttctttat ttttttgtag tcacgctctt
     6001 tgaaaccgac catgcaccgt aatattttgt gccgttcact gtcgtgtagg aacggacacg
     6061 aacgtaatat ttctttttgc cgctaagttt ggaaattgtg gtcttgtcgg tcttgttgtt
     6121 tgttatggtg actttctttg cgtttgtaaa cttggagtta gttgcatact gtatctcata
     6181 gcccgttgca gagcctttct gcgcccaatc cacnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnngcagagc ctttctgcgc ccaatccaca aagaagcctt tgtatcttgt
     6361 ttcaagcttt tgtatggtct gcttggcagg gataatgtca aagcctgcgg aataactgcc
     6421 gtagtagttg cccatgcctg agatagtgat gttagcgtga ccaattttaa cattgttcgt
     6481 gactatcaca cgatagtcga tgttctgacg tagaactttg cttccgtcct taacagtgac
     6541 tgtcggaata attgcactgc ctgtgtactg tgcagtgctt gcgtaggaat agcctgatgt
     6601 tggaagcttt tttgtggtga cctttacagt gcagacggca gaccttccgt taggcaggga
     6661 ggctgttatc tttgctgtgc ctttgcccgt ggcagtcaca acaccgtttt ttactgtggc
     6721 aacggctgtg ttggagcttt tccatgttac tgtctgagga gtgcctgagg tcacgctggc
     6781 ttttagggta atgctgtcgc cgtatgtgac ttcttttgac gtggagtcaa gagttatctg
     6841 tgtattgtca ctgctgatag gatagctttt caggtcgggc agacttgaaa gtgtcacggc
     6901 atagtcgctg ttatagacct tttgccacat acttttctcg gtatatttct cagagcttga
     6961 tatgcaaaat tcaccgctcc atgtgccgaa atatgaccat gcggtgtttg catcaaaggc
     7021 tttgtcaagg tcgaaaagac agccgttttc tgtaagggca actatttttc cgtttggtga
     7081 acagtctgtg gcttcaagat attttgaact ttgtgctgaa taatctcttg tgccggggta
     7141 gatatcctct ccgattatgt caacatattc gtcgccagga taccaatttg ccgcctgacc
     7201 attccatacc catatgatat tgtcaagctt gtattcattc gtgagcttgt catatattgc
     7261 ctgccaaagc tttttgtagg cttccgagcc gtctgcaccc caccagaacc aaccgccgct
     7321 tgcctcgtgg agcggtctga acagtaccgg aacgtctgcg tcagaaagac gtttgagttg
     7381 tttggcaatc tcgtcaacgt cgctcataag agtgttgtag cccttagggt ctgagccgtt
     7441 cattattttg gtaagcgaga actttgagcg gtcaacgttt ttggtgtaaa aaccacccca
     7501 ccaacgtgga tttccgccgt ctgtacctga gagaagatat ttgtcaggga cgttccaatg
     7561 ccagcagaat gttactattc cacctgcccg tgagaactcg atagcctttt cgacagcgtc
     7621 ggaggtgtca ccattctgga cacggcatgg agtatagcgc ataaagtcaa gacccaacat
     7681 agcaggggtc tgaccagtgg cggactttat agctttgaac tcagtgccgt taagaccacc
     7741 gtcgcaatac tgtcctgtta tgaccttttt gccgtaaacg tctttcatat agctgtaaag
     7801 gcgctgtgtg ctttgtgaag cgtatgggtc aacgagattt tcagcggcag tgtaggcatt
     7861 tgaagagctc attttttcaa tggttatgta gtcgagagta aaatatcccc acgacattgt
     7921 tattttgaca gtgttctgac cctttttgag ataaatacca ttcactgtgg actctttata
     7981 ggcagagccg tttgcactgc tgaaagtgcc tgcatttgag ccgtttacag aaaggttgtt
     8041 tatcttattc gagccttccc cgcggctgta aagctttatt ttgtagtatc cgctttcgct
     8101 gactgtaaca gtctgtgacc atgtgcagct tgagtctgaa aaactaactg acctagagcc
     8161 tgatgcggag ctgtctgttt ggatataggc agaaccgccc aaagtgccgt tttcggcttc
     8221 gtatttaagc gtctgctgtg caaaaacaga aagctgagcc gagcctgtgg tgagcattgc
     8281 cacacttaaa aatgcggctg cgaccttttt gatattgaac ttcatagatg ttttttcctt
     8341 gttatattat ttactgataa agctaagagc ttaaaagcat acatttattt tagcataatt
     8401 tttgtgaatt gtaaacaata tatcaaggct aattataaga tgatttgtaa actttaattt
     8461 tgcttatttc atcagccaaa tttcgcagac gtattcggaa gaggatgaat aatcaccgtt
     8521 aggttttagc tggctacgac acatatcata tattgcgcag ccgagatatt tgtaatccag
     8581 cctttgggaa taaactgtaa ggtcgccttc atcatcagtg aaaagctcgt ttgaaaaatt
     8641 tacttttttg gcatactcaa atacttcatc atagctttta cagcagttca ttacatagct
     8701 tacccttata aattcactgt ctgagggaag tccaacgtcg ctgaggttgc tttttagctt
     8761 atctaatgtt atttcattcc aagtatcgat ctcgccctcg ctttgtacat tagtcattgt
     8821 ttcgaggctt aatgctaaca ggctgtcaag ctgtccgcag accctcagag gtgcatagcc
     8881 tctgccttca cgttttttat ttattttgtc tgcaaatatt ctgcttagct catttgctcc
     8941 gtcctcggaa taatatgacg gctcgggctc ggctggagct gtgttatcct cctgctgaac
     9001 gttattttca tcaggctgat tttcaccatt attttcttca ggctgagttt cttcaggcag
     9061 ggttgttgta gtggtagtag ttcctgctgt gcctgtggaa gcctgcctaa taccgcctga
     9121 aattatgtct atctcatctg ccgttggtgc aggtgcggtg gtggtagtag gtacagttgt
     9181 tgtggcggca gtcgttgtct gcggtatggt cgttacagtg gcagaagttg tcacagcttt
     9241 tgcaggctca ggtgttttag tttctacggc attgtttttc ttgctgacaa cggcaaacac
     9301 tgacgatgcg gctatgatag cgactgcaac acaaaccatt ggtatggttc ttttgcgtga
     9361 cgtgtttacc tttataagta tttctttacg ctcgttgacg atggacatac aggcaaagca
     9421 gaaagtagaa tcatcgtgaa gctcgctgcc acagtgagga caagttctca ttttttacgc
     9481 tcccttgatt tggttatttt aattctatca cattttttgt aaattgtaaa cagtgcatag
     9541 agaaatatca tggacaaaat aattgttaaa ataaaaaaat atttttcgaa aacgtttaac
     9601 taagctgaca gcttaacaaa tttgattttt aatgtgctat aatgaataca acgataatta
     9661 gttacagtta aaatacaaaa taaaataagg ctagggagga aaaattatga acagtaagaa
     9721 gatagtagca ttgcttctgt gtttgggatt gagttttcaa tccactgcat tgagtggtgt
     9781 gttgtatgac gaaaatgctg tggtgaaagc ttcggctgaa gaaagcggca aaacggctga
     9841 tgcagagagc tatagcggta ctgagcagtc agaaaacaag gcacagcagc cagaaagcag
     9901 tgcagacaag gctgacaaaa ctcagcacgc ttatgaccct atctcggata tggaggctga
     9961 acaggaatat cgtgacagca cggagataac tcctggcaaa gtgctgttct cggtgctttc
    10021 ttatcatgca gagggtgaag aatcggctta tcttgatgat gacagcgata tctgcgccga
    10081 gtatgatctg aaagacgttg agtttattct tgaaacaaag gcagaaaaca ctgccgaaaa
    10141 ggacggcatg accccttata agacgttcta tgaagcgacc acctcggagg acgtttgggc
    10201 aactgtggac aagctgtcgg acaatgaaaa cattctgtcg gctgagcctg actttgtgtg
    10261 ggaaaagaca gacacaacaa ctgtggcggc aaccgatgag gaaataaacg ctgaaactca
    10321 ttttccttgc atggacgtaa caggcgtatg gaaagattgt tttgatcctg ctaaaaagga
    10381 agcccccggc aaaggtactg ttgtggctgt tatcgacacg ggcgttgact atacccataa
    10441 agaccttgca gacaacattt gggtgaatga gggcgaaata cccggcaacg gcattgatga
    10501 tgacggcaat ggctatgttg atgatgttca tggcgtagac tttgttgacg gcgacagtga
    10561 ccctatggac gagcatggtc acggcacaca cgttgcaggc ataatcgcaa tgacccctgg
    10621 caatggcggc ggagtcggtg ttgcatatgg tgcaaagata atgtgcgtaa gagcaggtca
    10681 ggcgaacgga agctttgcaa gctcggatat cgccaaggcg ataaagtatg ccgctgacaa
    10741 tggcgcagat gttatcaata tgtccttcgg tggaacaggc aggtcatatc tcgttgaaag
    10801 cgcattgcag gacgcttttc caagctgtgt tctcgttgcg gcggcaggta atgacggctt
    10861 gccgacaaat gacgcaaagc aagcgggcta tctgtttaca gaggatatat acccagcagg
    10921 atataagtat gttattggcg taatggcaac tgacaataat aagagccttg cctatttctc
    10981 aaactgggat tttaaagagg gttcgggttg tgaatacgaa atggcagcgc caggtgttgg
    11041 tatatatagc acattgccag gaaacagata tgcttgttgg agcggtacgt caatggcaac
    11101 accaaatgtt gcggcggcag cggcgatact cagaagcaag tatacagata aatcaaagta
    11161 tagctcaaga tatataatgg gtcagctttg cagtgccact acaagcaggg catatggatt
    11221 tcgtagttat cctgaactta atatccgtga tagcttaaca agacaagcac atcctaatct
    11281 gacacttgat gaagtctaca ctcttgatta tcaagatgtt tcatctgcaa ataatggcga
    11341 cggcattgca cagcccggtg aaactattga catgggtgta gcggtatgga acaggtgggg
    11401 tgcggcaact gatgttactg taaaagttga cgcaatctca gacggcggcg tggcaaacaa
    11461 atacgttgag ttcataaatg atgaagtcaa actgtcagat gtgggtacgt ttgcttctgt
    11521 aaataatggc tttacatata cagatgaaaa actcacaggc atatcaaacc ctatccgttt
    11581 taagataaaa gagggtacgc ctaatgatat tcaaataact ttcaatttta ccattaccgc
    11641 aaagaatggt cttgatgaaa aagatacagt tgtgtataca ataaaaccaa agcccacata
    11701 tactttaact gttcaaaatg gcattgcact cagcggaata atcaatgagg atatgaccct
    11761 tacaaatgat aagctttgga tacttgaaaa caatgtgctt atccctgagg gcgtaacagt
    11821 aacagttgag cctggcacaa ggatacagtt ctggtctgct gaccctaata ataagtatgg
    11881 tgcaagtgcc aacgtttatt tgcaggtaga gggcagattt attgccgagg gtacagaaga
    11941 acagcctatc gagatgtatc ctggtaaagg atttgaagat aatgcggtga caattactgg
    12001 aacgaaaaat gataactatt ataaagatag tggtctgctc gatgatgatt atgttgagaa
    12061 atatgcgtca ttaaaatatg ttaatatata taatgcgaat tataccagct attcagcact
    12121 taaaggtagg ccttttaatg ttactataat agatcattgt aattttatgt ataattgttc
    12181 atttagtttt ggaacgcttt atgccaaaag cattgcgaat tctcatttct ctaacatgag
    12241 tatgaaatat caaaactatg gtatagaggc taatgacatt acaactactc tttttgacaa
    12301 ttatgacaac caaagagtat atccttatga tctttttgaa aaaaatgtag cacttaatta
    12361 tcatggtaag aaagatgagg aaccttcaat aagagttggg gcatcagtta ggcattataa
    12421 tctactgttc gcaaataatg ccattcttaa ttcgttttat cttaataatt atgataatat
    12481 aagagcaatc gggtcctttg atgcttcaga caatcgtacc accttcgaca tctccggcaa
    12541 ctattggggt acaacgaacc ctgaccttgt aaaggtgcag tgctatgatg ccgattggaa
    12601 cgtttcaaaa aatgacctta tccaagagcc ttatctcaca cttgatgacg atatgtcgga
    12661 aatctaccca ttcgtcaccg aggcttatct cactgataag gacggcaaca ggatagacaa
    12721 ggtcagcggt gcacagacag ttaaaatgca cgttaagttc aatcgtgata tggcttctga
    12781 tgttcagcct atggtgacat acggaggaag cgagccttat actgatttcg cagtatcggg
    12841 cgattgggcg aacgcccgtg aatgggtaag cgagttcaag atagaccctt atatcgacct
    12901 tggcacaatg tatatccgtg taaagggagc tgcggcggct gacgataagt ggctcgtcac
    12961 aggtacagat tggggcagat ttaagtttga gataacaaac agctcggcac agtctatgtc
    13021 actcacaggc gagggtcagc ttggcaagaa ctttcttagc tggatgcagg acgattatga
    13081 tacccttgca ggctacaata tttacagagc aacaaaatat gatgctgaca aaaagcctga
    13141 gaagcagaat ttcacaagag tgaacaagtc tgttcttaat gcggacgagc tttcgttcat
    13201 tgacgaaaat gttcgggcag gcacagatta ttactattat ttcacagtgg tggatactga
    13261 ccttaacgag tcaaaggctt caaacgttat aagctgttgt ccattagagg gtgagccgcc
    13321 tgttattgta cacacccctg tgaaaacttt gacgccagac aaggctatag ctataacggc
    13381 tgatattacg gataatgtta aggttgcggg agcaacgctt tactataaaa ataagtctgc
    13441 cgacagttgg aagtctgttg atatgagaaa taccacaggc tcggtttaca aggcaactat
    13501 tgcggcttat gaggtcacag acgaggagat actctactat atcactgcta cagacggcgc
    13561 aaatgttgtt tactgtggca cggctgatga gcctaatgtt atagtaccgc actatctcac
    13621 aagaaagata acaaaaaaag caacttgcac agaggacgga gttattacat acacttgcac
    13681 agaatgtaat gaaagctata cagagaagat acctgcaacg ggtcataagt ataatgacgt
    13741 agtaacagag gcttcttgcg ataagggagg ctatacgctc catacttgtg caaactgcgg
    13801 agatacatac aaggatgatt tcaccgctcc tacaggacat aagtatacaa agacgacagt
    13861 taagcagcct tcatgcgata cagacggagt tagtgtttac acttgcgata cttgcggtga
    13921 cagctatagc gaagtaataa aagctaaagg tcatatctat gcttctgagg taacaaagaa
    13981 agcaaactgt acagacgacg gagttatcac atatacttgt gcgatctgcg gagataaata
    14041 cacagaagtg ataaaggcaa agggacacaa ttattccgca gaggtaacaa agaaagcaaa
    14101 ttgtacagac gatgaggtta tcacatatac ctgtgcaaat tgcggtgata agtacacaga
    14161 agtgataaag gcaaaaggtc acaactattc agcagaagta acaaagaaag caacttgcga
    14221 tacagacgga gtaaagacgt tcacttgtgc agattgcgga gatgtgtata ctgaaaagct
    14281 ggaagctttg ggtcatacat atggcatttc agaggtagta aagccgacct gcgataatga
    14341 cggctataca aagtttactt gttctgtatg cggtgacagc tacagcaagg tgatcaatgc
    14401 aacaggtcat aagtacaacg ataagatagt ttcggcttct tgcgataagg gtggctatac
    14461 gctccatact tgcgaaaact gtggtgatat atacaaggat aatttcactt caccacttgg
    14521 tcatgactat acgtcacaga cagtaaagcc agcctgcgaa acagacggag aaaaaacgtt
    14581 tacctgcaca cgctgtgggg atacatatac agaggtcata aagaccaagg gacatgttta
    14641 caagagaaca gttgtagcgg cagactgtga aacagacggc tacacgcttg ttgagtgtat
    14701 ggagtgccat gacagcttca aggaaggtta cgttggggca aagggtcata ctatcgttac
    14761 agacaaagct gttgcggcaa cttgcaccac cgctggcaaa acagagggtt cacactgttc
    14821 agtatgcgga aaggtcataa aggcacagac agaaataaag gccaagggtc acgttgcagg
    14881 cgactggata acagacaagg ctgcggctgt gggcgtaaaa ggaagaaagc acagaagctg
    14941 catggtatgt ggggcagttg tcgagtcagc tgatatccct gcactttctc caaagagcat
    15001 ttcttctgcc tctgtatcac tttcgattgc gacctattct tttgacggca agtttaaaac
    15061 gccgtccgtg actgtcaagc ttggcagtac agcacttaga aagggcattg attatgttgt
    15121 ttcatacaga aataataaga acgtgggaaa agcaactgtt gtcataactg gaaagggctt
    15181 atatgcagga actataacaa ggactttcgt gataaaccct gcaaagcagg agatacagaa
    15241 gcttacagct aagagcaagg gcttctatat cgactatgcc gcaaagggtc atgcgacagg
    15301 atatgagata cagtatgcta caaattcttc attcagcgga gcaaagaaaa cggtaataac
    15361 aagcaataag acagataaag taacagtttc aaagcttagc ggaaataaaa aatactatgt
    15421 aagagtaaga acctatacca cagtgaatgg cataaagtat tacggtgcat ggtctgctgt
    15481 aaagacagtt acaacaaaga aatagtgcag agtatcagat tgccgatgtc ggctctggcg
    15541 ttttgcagcc cacagacaac agctctgtgg gctgcttgtg ttaaaggcat atttgacatt
    15601 tcgtcattca tgtgatataa tcatagtgtg agcgagctat gcgatagcgt aaacgttcta
    15661 ggtttatgag gaaagtccga gcatcataga gcaggatagc tgataacgtc agccgagggt
    15721 gaccttagag ccagtgcaac agaaatatac cgccaacttt tgttggtaag ggtggaaagg
    15781 tggggtaaaa gcccaccaga atggcggaga cgtcattgct atgtaaacct tatccgatgc
    15841 aacaccaatg gtagcttgca agtcaatggc tgctcgtcag acttggggct ataggtggct
    15901 agagctgtgc ggcgacgtac agtccagata gattatcgca ctcgacaaaa ctcggcttat
    15961 cggttcggtc acatttgctc cgtcagttaa acttttttaa gcttaactac ggggcttgtt
    16021 ttttaataat atataaattt ggtcatttgt tttgtgcatt ataaataaat ttctataatt
    16081 ccccttgtga ttttacggaa aatatgttat aatgatgcta gacgtatnnn nnnnnnncgt
    16141 atttatataa gcagaggagg gctgtttgta tggaaatacc agctaagacc tataacgaaa
    16201 agttcgagag ctttttgaaa atacttgaaa gcgatgacag atttgataat gtgtgcaaga
    16261 attttcttga aagtatttgc tctgcacttt atgtcaatgc ggctacggta acagtttcag
    16321 gctatgaaaa ctcagaacct ttgagttatg aactgatcgc tgatacacat aaatatactg
    16381 tatttgcttt tgataaagaa tttaatagaa atagatacaa tgcaatgaaa aaagcactta
    16441 tgcaaactgg tattcacgtt gcggataagg ctcatccgaa tggagatgcg gaggaaatat
    16501 ccctgcgtgg tgtggtgtat ccttgttcac tcacttgtgc tgtcaaggac accaataagt
    16561 atgttgcata tttcaatctt ttgtcctctg atcctgagag agtatggtca cctgaggaga
    16621 tacagctagc ctcacgtctg acatatatac tttctggcat gctccatact aggagagcgg
    16681 ctgaacatgc tgaaagcact actcgtacgc ttactgacgt tatggataat atgaattcct
    16741 atgtgcttgc agtggcacag gacagtggtg agatagtttt tgccagcaag agctttgaac
    16801 agtatttggg aaaactggtc ggcaaaaagg aaagtgacgt tttcagcaag acttggtgtg
    16861 agctttctgc tcataaggaa ggaatgcttt ttggaggaga ttattattcc gagatcatga
    16921 acaaatggtt caatgtgtcg gaaaaactca taaaatggca tgacggcaga actatcagac
    16981 tttgcacact taatgatatc tcagaaaaga taaattatga gagggtcatc gaaaagcagg
    17041 tcttttacga taacctcaca ggtcttccta acagattaag tcttgataag agtatacagg
    17101 gcattattga aagcgatgag aacagcactg ttatgctcat tgaccttgat aatttcaaaa
    17161 acataaatga cagctatggc cacaataatg gtgaccgact tcttaaagag ataggtaaat
    17221 tctttgtcga ttttgcccag aagaataaaa agctgagcat ttttagattt ggcagcgatg
    17281 agttcgttgt gcttctgaga gatcaggatc aggccgagat cgacgagata tgcaagatca
    17341 tacatgataa gttcaacaca gagtggcaga ttgctaacag cacttgttat tgcacttgtt
    17401 caatgggcgt tgcgaagttc ccgaagcttg acgatactgt aaacacagtt cttaaaagga
    17461 tcgaccttgc catttcatat gcgaaaaagc agggcaagaa ttgtactata aattataacg
    17521 aaactatcgg caggatactt tcaaggcaga tcgaacttga aaggctcatg cacagggata
    17581 tcacaaacgg ctttgcacat ttcacggcgt actatcagcc tatattcagc agctccgaaa
    17641 agaagcttgt gggctgtgaa gcgcttatga ggtggaatcc acctgagttc ggtcaggttt
    17701 cccctgccga atttatccct atggcggaaa ggcttggcta tatcaatctc ttgggcgaga
    17761 acataataag gatcgcaggc agacagtgca aaaagtgggc tgacgcaggg cttgatttga
    17821 gaatgaacat caatctgtct gtaggtcaga taattgagcc tgatttcctt gacaagatag
    17881 acacactttt tgacgaaaca ggcgcacccc ctgataagat atgttttgag gtcacagaaa
    17941 gtcttgctat ccatgatatg ggaaagatga aggagctttt gaagcatata acagacaagg
    18001 gcgtgaagat agcactggac gatttcggaa cgggatattc ttcgcttaac tgcataaagg
    18061 aaatgccgct gaataccatt aaaatagaca agtcgtttat tgatgatatt gcaaaaaatc
    18121 ctgacactgc ggtgtttgtt aaaatgatag ttaatctttc tcacgacctg catatagcag
    18181 tttgcgcaga gggcgttgag gaacaggtgc agttcgatat cctgagagat ctggaaactg
    18241 acgttataca aggctattat ttcggcagac ctatgccgtc agacaagttt gaggagaagt
    18301 tccttagcga gaaactccag tcagtaaaat aagtcgccaa aaaaagaaag ttcgaccgtc
    18361 gggcgtaata ctcggcggtc ttataaatat gtaaaaatca aaaataaagg ggaaagctta
    18421 tgagtgatga gaaaaaagac gtaaacagcc agcaggagaa tgtgggcaaa aagggcataa
    18481 acaaaaagct tatcatcgct tttgcaggca tgggcgctgt tatagtgggg cttgttattg
    18541 cactgatact ggtggcaaca tcaggtaaaa aagacgtgga cagttcggct tcaaaggcgg
    18601 agagcagttc tgcggtggct tcagaaaccg atgacaccag tgaggaggac tctgtggctg
    18661 atgaaagcga ggacgagcct gatgctgatg aggaggaaag ctctcaggaa agcaaggacg
    18721 ataaaaagga aaagggcttt atcatctcct attcagggga caacagttgg caggacggcg
    18781 acaaggtcat gacaggcatg gagataggca ttgagaacaa ctccgatgaa gccctcaagg
    18841 agtggacgct ggagcttgaa atagaccagc tcaaaagctg tgacggctgg aacggcaact
    18901 ttaaggtcaa gggcaacaaa ctcacggtca cgaatgttga ttacaatggc gagatcgcaa
    18961 agggcggttc gtcctctatc ggctgtaata tcggaactgg aacaaagctc aatgttaagt
    19021 ctgcaaagct aaacggtgtg gagtgtactg ttaaaaaggg caaggtatca cagaataata
    19081 acaataacaa caataatcag aataatgaca aaaagactac ggaaaaggac gttaaaaagc
    19141 ttctaaagcg tacctcaaag gctaaacagg gtgatgattg gcttcacaca gacggaagca
    19201 agatacttga taaggacggc aaagaggttt ggctcacggg cgtgaactgg tttggctaca
    19261 acacaggcac taacaccttt gacggacttt ggaacagcga gcttgtgtca tcggtggagg
    19321 ctattgcaga ccacggtttc aatcttataa gagtgcctat gtcagcggag cttatcaacc
    19381 agtggtcgga gggcgaatat ccaaaggcga actacaataa cgcatacaat gaagagctga
    19441 actctatgaa cagccttgag atatttgatt attttctcaa gcttgcggag gaaaacggtc
    19501 ttaaagtaat gcctgatatc cattcagctg aaaccaatgc cagcggtcat actgtaaatc
    19561 tgtggtatac tgacaaggtc actgtaaaga actattacca tgctttggag tggcttgccg
    19621 acagatataa ggacaatgac actatcgttg catttgacct gaaaaacgag cctcacggca
    19681 agcctaacga gggtgacgct gcggcaatat ggaatgattc aaaggatgca aacaactgga
    19741 agtatgtggc tgaaacggca gcgagcaagg tgcttgcgaa aaatcctaat gtgcttataa
    19801 tggtcgaggg tatcgagata taccctaaga acatcaagaa aaacggggat tattcctcca
    19861 ccaacagtga tgattactat ttcaactggt ggggcggaaa cctcagggga gtaaaggact
    19921 atcctatcga ccttggcaaa tatcaggata agcttgttta ctcgccgcac gactatggtc
    19981 cgacagttta tcagcagcct tggttcgagg gggactacac atacaagagc cttatgaaag
    20041 attgctggaa agacaactgg ttctacattc aggagcagga tatcgcaccg cttcttatag
    20101 gcgagtgggg cggctttatg acagagccta acctaacttg gatgacctac atgagaaagc
    20161 tcataaagga caatcatgta aatcacactt tctggtgctt caatgcaaac tcaggtgaca
    20221 cgggcggact tgtaaaggac gattttgtta catgggacga ggaaaagtat gattttgtca
    20281 aggaagttct ttggcaggag ggaggcaagt tcgttggact tgacctcgct atcccgcttg
    20341 gagataatgg tataactttg aaaaaggcaa agggtctttg atatcgcata taaaaatagc
    20401 ggacgaagtt ttcgggcttc gtccgctttt gtattgtatt tttatcgctg gtcgcccata
    20461 aagaacagag cgagagttcc aggccctgag tgtgagccta taacagcacc tgtgtagcag
    20521 atatgcacag tggttttctt gccaagcttt tttcgcatga ggtcggctgc atattctgcg
    20581 tctgaaaggc agtcgccatg gcagacggct actgtggaat tatcccaatt tcccatgcgt
    20641 tcgcacatca tatccacaag cttttcaatg gattgctttc tgcctcgtat ctttccaact
    20701 gggataagat gtccctcgtt gtcaacgtgg agaagcggtt taatgccaag aatagagcct
    20761 gctatggcgg cagtctttga tactctgcca cctcggtgga gatatttcag gtcatcaact
    20821 gtgaaaacat ggcataggtg aagcctgttg tcagtgatcc attgggcaag ctcgtcaatg
    20881 gaaagaccct gctttttctt ttcgtcagcc tttatgagca aaaggccttc gcctgttgaa
    20941 gcacagaggg agtctatgca gattatcttg ccttctgggt atttttccat tacgctatct
    21001 cttgcaatgc aggaagtgtt gaagctgccg ctaagacctg acgaaaatcc aagataaagc
    21061 acgtcatagc ctgcggcaag ttccttttca aaagcctgtt catagtcgga gatacctatt
    21121 tgcgatgttt tcgttactgc tccgtttcgc atttgggaat aaaattcagg cagcgtcatg
    21181 tcattcttgc tctttacaac accgtttact tcaaaagata agtccagaag cgtcacgtcc
    21241 atatttttta atatatcctc cgacagatcg caggtggagt cggaatatag cttgtatttt
    21301 ctgtcagaca tttttatcct ccttttgccg cttgggcaac aatataaaaa tattataaca
    21361 tatgggcggg cttttgtaaa atatccctca actatacttt gtaaaatttc tatgaacaca
    21421 gttgtctgcg gtgctgtgcg acagttaagg tgttctcaga tacgcaaaca gaaaagtcac
    21481 ggcaggcgga ggagataagc atatcgctta tgcgattttc tatgagggcg gtaattatgt
    21541 ggcgtatctc tcgtgcagag ccgctttttg aaagtgcctg ctttgcgata aaagggtata
    21601 cctctggctg acaggcaagt gtacagccaa gttcggagag ccttttttca agagcggata
    21661 tttcaaggcg tgtaatgttt tccatatcgg aaagggtaag gtcgttaaaa acaatcacct
    21721 catctatcct gcctaaaagc tcgggagaca taaagctttt aagctctgag agcatggctt
    21781 ttttcttggc ttcgccattt gacttgctgt ttgaaaagcc cacggtcttg gtttcggcaa
    21841 gctttttcac gccgatattc gaggtcatta ttataaggac attggagaaa gaaactgtcc
    21901 tgccagcgga gtcggtaaga cagccgtcct ctagtatctg caaaagcaga ttgtagatgt
    21961 cagggtgagc cttttctatt tcgtcaagaa gaagcacaca gtagggcttg cggcgaattt
    22021 gttcggtaag cctgccaccc tcgtcaaagc ctacatatcc agggggcgag cctatgagct
    22081 ttgaaacgct gtggcgttcc atgtattctg acatatcaag ctttacaatg gcgttatctc
    22141 tgccaaaaag cgttttggca agatcttttg caagctggct cttgcccact cccgttgacc
    22201 ctaagaaaat aaagcttccc atggggcggt cgctgccctt tagtcctgca cgacagcggc
    22261 ggacggcaga gcaaagtgac tttatagccg tctgctgtcc cacaatgctt tcgcttagct
    22321 tat
//
LOCUS       DAWEMR010000018        21824 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1006_length_21824_cov_11.483945,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000018 DAWEMR010000000
VERSION     DAWEMR010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 21824)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21824)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21824
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1006_length_21824_cov_11.483945"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    14764..14773
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6582 a         4139 c         5244 g         5849 t
ORIGIN      
        1 acagttatcg ctaagaagca cggtggaatc cacgagacat tcacagttcg tagagtagct
       61 cacggctgtg gaatcgagag agttttccct gttcattcac ctgtagttga aaaggtagag
      121 gttgtacgca gcggtaaggt tcgccgtgct aaactctatt atctccgtga cagagttggt
      181 aaggcagcta aggtcaagga agctatcaga taattctttc tataaaaacg agtcggcttt
      241 ggtcggctct tttttttata tccacgactg agttgttcgc acgatatacg gtaggggcga
      301 acagcgttcg cctgcatata tcgcccgaca attttatgat attcgtacat tcgttatatt
      361 ttcacaaacc atgtagggcg tcgtccctac agttctgaat ttgtacaagt tctgagcatg
      421 ggcgaacact gttcgcccct acagattatt tgggatagtt tgtcttgccg caaaatcaca
      481 tgacatttca ccgtcacatt gcacaaaatg tcggtattcg atttgtgcaa acaaatgatt
      541 tatattttaa tttggcaaca tttctgcaat tgttgcattt tttatgcaac aaaacgccct
      601 tttttctgga taagtagagg gggataatta cctctgcttc acagagaaag gacgataaaa
      661 atggcaaaga attttcacac agacaacaaa gcgttcatgc tttacaaaga ctgggaggac
      721 atttttcagg cgcttgacag cagcgaagag ataggcgagc ttgtcatggc gctgtttgct
      781 tatgcaaaga ggggagaaac accaaagctg tcaggcggag caaagatagc gtttcttatg
      841 atgagggctg ctatcgagcg tgacggcatt gcatgggaag agatgtgtgc tcaaaggagc
      901 gagtgcggta aaaagggcgg cagacctaaa aagtctgatg agtcagggaa tactgatgaa
      961 aaagctgagc agacagctgc atcagcagat aatgcagaag aaaaacctca gggtataaat
     1021 gataccgcag aaaaaccaaa agctttttca caaaccaaaa ggttttcaga aaaagcaaaa
     1081 aaagctgata aagatataga tatagataaa gacaaagata cagatataga tatagacaaa
     1141 gatacagata aagatacaga taaagacaaa gataaagata aagctataga taaagagaaa
     1201 aagaaagacg cagggacagc gaagtctgtc gtcaggtctt tcggcacaca caaaaatata
     1261 atgctcacat cagagcagta taacgagctt gaaagcaggt gtcggtctgt cggtctgtct
     1321 ttagagagct atatatctaa attatctcaa tggctgttta ccaagggcag gcactgcaca
     1381 gacccatacc gcataatctc aggctggctt gacaatgatt caaagagaaa gactgaggca
     1441 aagcccaaag aaagacaagg ctgcgatctg tcgtggtatg atgactttgc catgaatttt
     1501 gatctgtcaa agttttttta ggcaatatca aaaagaaatt tatctcaccc ccttgtaatt
     1561 tgttttgaaa tttgatataa taaatctata tgagtatttg acggcttttg ccgcctttgg
     1621 ataaaggatc gaggctttta tggacgataa taaaaaattc ggcgacgttt ctcaggagga
     1681 tattgataag gctaaaaaag ccgcccctga cgacctaaag gaaaaacagc ccgtgaatgc
     1741 tatggacgag gtctttgaat gggctgaatc tttcgtgttc gctatgtttg tggtgatcct
     1801 gatatttact tttttcttca ggatagttct tgttcaggga ccttctatgc gtgagacctt
     1861 gcaggatcag gacagactta taatcactca cataaattat acaccgcaaa agggcgatat
     1921 cgttgttatc aacagcgaaa agcttggcaa aactatcatc aagcgtgtta tcggcaccgg
     1981 tggggataag gttgtcgttg attataataa caacaccgtt acagtaaacg gtaaggtcat
     2041 ttcaaatgac aatatcaggg aagctatgta taacactaat ctctttgatg agaaatatga
     2101 agtggaagaa aatgtttttg agtatgacgt gcctgagggc aagctgttcg tcatgggtga
     2161 taacagaaat aattccacag acagccgaag gataggcttt atcgacccat ctgacgtgct
     2221 tggtaaggca gttttaaggc tttatccatt tgacagcttt ggtaaggtaa gttgaaaaag
     2281 cagggtaaaa gagctgttga taatctgttc gactttcttg agatcatagt catggcaatg
     2341 ttcgtggtcg tgcttgtttt tacttttgtg tttagaacag tgaatgtaca aggacagtca
     2401 atgcaggaca cactttttga aaatgattct cttgttatat caaaactgct ttatacgcca
     2461 aagcaggggg atatagttgt ggtcaactcg tctgttcttg acaataggat aatcaaacgt
     2521 atcattgccg tagaagggca aaaggtcgag atagacaaca agtcggctat agtcaaggtc
     2581 aacggagcag tgcttgatga gagctatatt aagtgcgacg gtagctttga tgacgagtat
     2641 tttgatgaaa gatactataa taaagaaagt gaagtttatg aatatgaagt gcctgcggga
     2701 tgttgctttg taatgggtga caaccgaaat cattccacag acagccgtgt aataggatat
     2761 gtgccttatg acgagatagt cggcagagtg ctgtttcgat ttgcggcagg caatgatgag
     2821 ggcgttggta aggtagattg agttagagta aggaggaaga tatgagcgaa gtatctcacg
     2881 ttcagtggtt tcctggacat atggcaaaaa cgcgccgcat aatggcgtca aatctaaagc
     2941 ttgtggacgc agtggtggaa ataactgacg ctcgtatccc gttttcaagc agaaaccctg
     3001 aaatgaagcg tatcgtaggc tcaaagccaa ggcttgtgct tctcaacaag gctgattcag
     3061 ccgatccgga agtaacggca atgtggctcg actattatgc aaaacaggga atcacagcac
     3121 ttgctacaga ttgtaggagt ggcaaaaacg taagtaagtt ttatggaaag ctcaaagagc
     3181 ttttaaagga cgatattgag aaatggaatg ccaagggtat gtctggcaga cctataagaa
     3241 taatgatagt gggtatacca aacgttggca agtcgtcatt tataaacaga cttgcagggt
     3301 caaggcttgc aaaagttgag gacagacccg gtgttacaag aggtaaacag tgggtgaagc
     3361 ttgacgaggg ctttgaactt ttggatatgc cgggtgtgct gtggcctaag tttgaggata
     3421 agcttgttgg cgaaaggctc gctttcacag gtgcagtcaa ggacgatata atggacatgg
     3481 aatatctcgc ctgccgtctg cttgagtttt tgaaagagaa ctacactgag cttatcgaga
     3541 aacgttatgg catcagaaca gatgatatag agcctgctga cgaggaaact atcggctgtg
     3601 tggtaggctt tgagcttctt gaaagaatag gcagaaagcg tggcttttta gtttctggcg
     3661 gagagataaa taccgaaagg gcagccatta cagttatgga cgagttcaga aacggtactc
     3721 tcggcagact tactcttgaa aagccaaagg ggtattgata tgacgctcag cgagtttgac
     3781 aacgagataa gaaaagatta tgacgtggtg tgcggctgcg atgaagctgg cagaggtcct
     3841 cttgcaggag atgtgtttgc agcggctgtg atatttgata aggacacagt tatagagggg
     3901 ataaacgaca gcaaaaagct tacagcgaaa aagcgtgagg agctttttga tgagataatg
     3961 gaaaaagcag ttgacttttc tatacagacc gcaacagtgg aagagataga ggatataaat
     4021 atacttaacg ccgcaatgct tgcaatgaaa agagcggtgg aggatatgga cacaaagcct
     4081 gatgtttgcc ttatcgacgg gaacaagacc cctgaggggc ttgaatgtcc tgccatagcg
     4141 gtggtaaagg gagatgccaa aagtcaggca gtggcggcgg cttcaattct tgcaaaggtc
     4201 gcaagggaca ggtatatgga gcaaatggct gaacaatatc ctgagtatcg gtttgagaag
     4261 cataagggct atggcacaaa gctccactat cagatgatag accagtatgg accatgcggg
     4321 atacatagaa tgagttttct caaaaagtat tatgcaaaga aaaatgggca ttgaccatac
     4381 ccagcgccga aaggcgggca atatcgggga ggacgcagtt tgcggctttc ttgtaagaca
     4441 cgggtatgag ataataaaga ggaattttac cgtcagaggc ggtgaaatag atataatcgc
     4501 agaaaaagcg gatatcatag cttttgttga ggtgaaaaca agaaccatag gctctatgac
     4561 ctcagcggag gaagctgttg accttagaaa acagcggctc atcatcagaa ccgcgaaggc
     4621 ttatttgcaa aatgttgcac agcctttgca atgcagattt gatgttgcca cagttgaaac
     4681 tgagggtgga aaggtgaaaa agctgagata ttatgtgaac gcttttgatg caagtatgaa
     4741 ataaagacca tgggtcggat atgccgaacg aactgtgtct gtaggggcga acactgttcg
     4801 cccgtgacgt ggaatgttag acgttatctg aacaaatggt ctgtaaatag ccgacacctg
     4861 tcggacatct aaaacaagaa gggttgttgt ttatgtttta caaaagtaca agaaacagcg
     4921 aagtaaaggt ttcgtctgca caggcgatag cacagggcat ttctgaggac ggcggtcttt
     4981 tcgttccgtc tgagatacct gccataacac ttgatgatat caaggctctt ggcgaaaaaa
     5041 gctacgctga gagagcattt ttcgttttct caaagtacct cacagatttc acagaggctg
     5101 agatacgcta ctgtgtagag ggtgcttaca acacaaagaa tttcgacact gacaatattg
     5161 cagagcttgc tcaccttttt gacggcactt atatgctcga actgtggcat ggtccaactt
     5221 gtgcatttaa ggatatggct ctccagatac ttccatatct gctcacaact tctatcaaga
     5281 agcttggcgt tgacaagaag gtagttatcc tcgttgctac atcaggcgac actggcaagg
     5341 ctgctctcga gggcttcaag gacgttgagg gtacagagat actcgtgttc tatccagagg
     5401 acggcgtttc agctatgcag aaaaagcaga tgacaactca ggagggcggc aacgtcggcg
     5461 tttgcgctat caagggtaac tttgacgacg ctcagacagg tgttaagact atcttcacaa
     5521 gcaaggaaac tgccgacaag cttgctgaga acggcatgat gttctcctct gcaaactcta
     5581 tcaactgggg cagacttgca ccacagataa tctactatgt atctacatac gctcagcttg
     5641 ttgctgatgg cgagatagaa cttggcgata agataaatat agttgttcca acaggcaact
     5701 tcggtaatat ccttgcaggc tactatgcaa agcatatggg cgttcctgtt aataagctta
     5761 tctgcgcttc aaacgcaaat aacgtcctca cagactttat aaagacaggt atttatgaca
     5821 gaaatcgtcc gttccataca actatctctc cttctatgga tatccttatt tcctctaacc
     5881 ttgaaagact tctttatcac ctcagcggtt ctgatgacgc tcagataaga gagtggttcg
     5941 gcagcctttc caagactggc agatatgagg taacagacga agttaagaag gctatcgctg
     6001 acgagttcta tgcaggctgc tgcgacgatg agcagacaaa ggcttgcatt aaggagatct
     6061 atgacgagta ttcttacacc tgcgacactc acacagccgt tgcagtgaag gtgtacaagg
     6121 actatgcagc tgctacaggc gacaagacaa agactgttat cgcttcaaca gcaagcccgt
     6181 ataagttctc aggttcagtt cttgcagcta tcggtaagga tacaaacgct gacgagttcg
     6241 gtcttgttga cgagcttgcc gaggtttcaa agatacctgt tcctgcttca cttgctgcac
     6301 ttaaggataa ggaagtaaga ttcacaaagg tgatcgacaa ggctgatatg aaggactaca
     6361 tctactcagc tctgggtatt tgatatgtac ccgtccgact attgtcgggc ggaggtcagc
     6421 gcacagcgta tgtccactga ccatggcgag gggaattagc ctcttgcctt gaatgtcttg
     6481 cactctgttt cgtcacagca gttagggtca gtgcaggtac agcctacatt gatcttgcct
     6541 gcggtacatt cgcagttgtt gctgttgtaa acgcagttgt ctacgttgca gtggataccg
     6601 taaattctat ccttctccat ttgtacatcg ctccttctgc agctttttgc tgcaaaagta
     6661 gtatgtgcat gagtggtcgg gataatacat gaagtttctg gaatggagga aaaaccgtga
     6721 gcctatttgc cattgccgac ttgcatttgt cgctgggtac agataaacca atggatatat
     6781 tcggtggctg gtctgactat gtgacaaagc ttgaaacaaa ctggcagaac aaagttcgtc
     6841 ctgaggacac tgtggtgata ccgggtgata tctcgtgggg aatgaatttt gagcagtcga
     6901 aaaaagactt cgagttcata aataagctca acggcagaaa aataatatcc aagggcaatc
     6961 acgattattg gtggaacacc aagaacaaaa tggataaatt tttcgcagaa aacggttttg
     7021 ataccataaa tatcctgcat aataatcatt acagctatga tggctacggc atatgcggaa
     7081 cgagaggctg gataagcgat aatggtgaac ctgcagatca gaaggttctg gcaagggaag
     7141 caggcagact tgcactctct atcgagtctg cgcaaaaggc aggcttagag cctgtggtgt
     7201 ttttgcatta tccgccgctt ttcggcaata gctgcaatta tgatatgctt gaagtgcttc
     7261 ataaatacgg cataaaaaag tgtttttacg gacaccttca cggaagagcc cacgcttatg
     7321 caataaacgg cactcgtgac ggagtagatt atcggcttat agctagtgat ttcttgcatt
     7381 ttgacccatt ggacataacg aaaattgtgc aaagtgacaa tttataaaaa aatgctggaa
     7441 atgacaatat atttgtgcta taatgtataa gatacattta tataatggag gaataataaa
     7501 tgagtttaaa agctacaaac aatgttgaaa ccaataagta cgagcttgag atcgagatct
     7561 ctgctgagga ttttgaggct gcaatagaga aagcttatct taaagcaaga aagaatatcg
     7621 ctatgcctgg cttccgtaag ggtaaggctc ctagaaagct tatcgaaaag gaatacggcg
     7681 agcaggtatt ttttgaggac gctgtaaatc ttctgtacgc tcctgttgta aacggcgcag
     7741 ttgaagagtc cggtctggaa cttgttactc gcccagaggt agaggtaact gagatcagca
     7801 aggaaaacgg cgtaaagctc aaggcaactt gcatcacaaa gcctgaggtt gaagttaagg
     7861 actataaggg tatagaagtt gaaaaagttg tcaatcctgt aactgatgag gatatcaaca
     7921 agcagcttga cgctctcaga gagaagaatg taactgttga gacagttgac gacagagctg
     7981 ctgagaacgg tgacgacgtt gttatcgact tcgagggctt caaggacgat gttgctttcg
     8041 agggcggcaa ggcagaagat ttcacactgt cacttggaag cggtcagttc attcctggct
     8101 ttgaggatca gatcgttggt cacaatgccg gtgaggattt cgacatcaac gtaacattcc
     8161 ctgaggagta tcaggtaaag gaacttgcag gcgcacctgc tgtattcaag atcaagctca
     8221 agagcatatc aaagaaggtt atgcctgagc tggacgatga tatggtaaag gattcaacag
     8281 agttcgatac agttgacgaa tacaaggctg acgttaagaa gaagcttgag gaagctaacg
     8341 agaagcacgc tgactctgag gtagaggcta agatattcga taaggtcatc gagaacatga
     8401 cagctgagat cccacaggtt atgtttgaca acagagtaaa cgagatgatc agcgagctgg
     8461 agcagagact tgcaccacag ggcatctcac ttgatctcta tatgcagtat acaggtcaga
     8521 ctatcgacac tgtaaagaag gcatatgctg agcaggctga gaagcaggtc aagctcagac
     8581 ttgcacttga aaagatcgca aagcttgaga atatcgaggt aactgaggac gagcttaaag
     8641 ctgagtttga taagcttgct gaggcttaca agcttgacgt tgaccagatc aagcagttta
     8701 tccatgatga cgaccttaag aaggatattg cagttggcaa ggctgttgac cttatcaagg
     8761 acgcagcagt tataaagtaa gataatttat gctttcgccc gcatgacctt atgcgggcga
     8821 aaatagatta aggaggcaat tttatggcat tggtacctta tgtagtggaa cagacaagca
     8881 gaggcgagag aagctatgac atttattccc gcctgctcaa tgacagaata atcatgctct
     8941 gcgaccagat agacgacgca gtggcaagcg tagtagtggc acagttgctt tatcttgagg
     9001 gacaggatcc tgaaaaggat atcgaccttt atataaacag ccctggagga gttatcactg
     9061 ccggcatggc tatctatgat actatccagt atatcaagtg tgacgtgtca actatatgca
     9121 tgggtatggc tgcttcaatg ggttcattcc tgctttcaag cggcacaaag ggtaagagat
     9181 ttgctctgcc aaacagtgag atactcattc atcagcctct tattgcaggc ggcggaattt
     9241 ctggtcagtg tacagatatc aagatacatt ctgaccacat ggtaaagacc agagagaagc
     9301 tcaacaggat acttgctcag aacacaggca agcctatcga gcagatcaac atcgacactg
     9361 agcgtgacaa ttatatgaca gctcaggagg ctcttgaata cggtcttatc gacaaggtaa
     9421 taactaacag ataggttcgg tgaaataaat ggctaatgat acaataagaa aatgctcgtt
     9481 ctgcggcaag cctgaggaca aggttagaac acttttttca gcaggcacga gccacatttg
     9541 tgacgagtgt gtactttatt gctataatgt tctggctgag caggagggac ttcctacaac
     9601 gaagcagcgc ggcaagggta aaaattcagc tgacagcggc ataaccctta agaagcctgc
     9661 ggagataaag gcagttcttg atgagtatgt tatcgggcag gaggaagcaa agatagcgct
     9721 ttctgttgcg gtatacaatc attacaagag aattttgtca ctcaatgacg atgatgacgt
     9781 agagatacag aaaagtaacg ttttgcttct tggaccaaca ggaacaggaa agactttgct
     9841 tgcacagact ttggcaaggc ttctgaacgt gccttttgct atcgctgacg ctactacgct
     9901 cacagaggca ggctatgtag gcgatgatgt tgaaaacgtg cttacaaggc ttattcaggc
     9961 ggcagattat aatgttgaag cggctcagaa cggtattatt tatatcgacg agatagataa
    10021 gatatcaaga aaatcggaga acgtttctat cacaagagat gtaagcggtg agggcgtaca
    10081 gcaggcactt cttaagatag tagagggctg tgtgtccaat gtgcctccac agggtggcag
    10141 aaagcacccg catcaggagt tcatacagat agatacgaag aatattttgt ttatctgtgg
    10201 cggagctttt gagggacttg acagtgttat caagaagcgt acagagtcat catcacttgg
    10261 ttttggcgga aaggtcaaga gcaaggaaga tgataacaat gtgatgaaga gggtagtacc
    10321 acacgatctt gttaagttcg gtatcgtacc tgagcttgtg ggcagacttc ctgttatcac
    10381 ggtccttgac gagcttgacg aggacgcact ttgcaggatc ttgacagagc caaagaactc
    10441 gcttatcaag caatacacga agcttttcaa gcttgataac atcgattttg agatcacaga
    10501 ggacgcaaag cgagagatag ccaagaggac tattgaagaa aagacaggag caagaggact
    10561 tagggcgata gttgagaaga tactcacaaa gctcatgttt gaagcaccat ctgatgatga
    10621 gatagttggc ataaagatta cggctgaatg tgttcgtggc gaagcagagc ctgagataac
    10681 tcgtgcggta agctcggcca gaaagaaata atagtacaaa tctttgtaca gataaattag
    10741 aggtggagta ttctgcctct tttttgttgc atttctgcgt tatccgcata ttatttattt
    10801 gtttaacaaa ccatgtaggg gctggcgccc tcgacagccc gctttttgga cgttacatct
    10861 tgtaatttat tgcacaaact ttgagaaaac acacaaattg tggtaaggac tagcattgtt
    10921 cacccgtttc ggtcgcttgc ataaatttgt cacaatcaaa aaaattcgac acttcgatat
    10981 cgggacgtcg ggggcgccgt cccctacatt ttgttgttta cgatacgagt tcatgaagct
    11041 aacgggcgac actccccact ttgaccgctt tttcccttga attatgcgga aatatgtgct
    11101 atactaaaga aaatacacta tagggggtat ctatatgaat gaacttatcc aacagtttat
    11161 tactgatgaa gtcactgctc tcacttactc cgacctttgg aacttcgtta actccaactc
    11221 catttgccgt ggtactttcg agggcaataa tcacattatc atgaaaatct cctctaatca
    11281 attcattatt tatcgcattt gccttggtat ggaaaacacc aaatatcagg aagctgttct
    11341 tgttgcaaaa acctacctgc ttaaaaagat aaatagcatg gcttatcagc ttcatttaga
    11401 ggatattcag aatattttag actaattgct ttttaaggag atcataaaat gagcgtgcct
    11461 ttggctgaac gaattagacc caaaaccctt gatgatattg tgggacagac ccacctgcta
    11521 ggcgaaaata aacctctcag gcgtattatc gaaagcggta aaataccgaa tatgattttt
    11581 tatggtccgt caggtgttgg caaaactacg gtcgcaagaa tgatagcaaa taatgctaat
    11641 atgactatgc ataagcttaa cggcacaagt gcttctattg ctgatataaa gcagatagtt
    11701 gctgaaactg agacactcag cggcattaat ggtatcttgc tttatcttga cgagatacag
    11761 tatcttaaca aaaaacaaca gcaatcgctt cttgaataca ttgaaaacgg caagataact
    11821 cttatcgctt ctaccactga aaatccgtat ttttatgtgt acaatgctat aatcagccgt
    11881 tcaactgtgt ttgagttcaa gccgctgact tcggatgaga tcttgcctgc aataaagcgt
    11941 gcttttcagc ttatggctga tgagttggga ctgaaactgc gtcttgaaaa tggtgtgttg
    12001 gagcatatcg caaggggctg tggtggtgat gtgagaaagt caataaatac agtggagctt
    12061 tgcatacttt cggcagataa tgacggtgaa aagctcaccg tgaaccttga taatgtgaag
    12121 cttcttactc agcgtagcaa tatgcgctat gaccgtgcag gcgatgagca ttatgatatt
    12181 ctgtcggctt tgcagaagtc cataagaggt tctgacgaga acgccgcact tcattatgcc
    12241 gcacggctta ttgaagcagg ggatatcatt tctctgtgca gacggctgct tgttatcgca
    12301 agcgaggata taggacttgc gtaccctatg gcggcggtag tgacgaaatc ctgcgttgat
    12361 tcggcattgc agttgggact gcctgaagca aggataccat tggcggaagc tgtggttctt
    12421 cttgccactg ctccaaaatc aaattctgct gaatcagcaa tagacgcagc tttatcagat
    12481 gtgagaaatg gtgattttgg agattatcca cggcatttgc agaataagca ttttgacggc
    12541 gagggcgcaa aagtgaaagg acagcactat ctttatcccc acgattatcc gaaccattgg
    12601 gtaaaacagc agtatctgcc tgataagctt gtggggagga cttattatca atatggtgag
    12661 aataagacgg agcaaatggc gaaagcgtat tgggagaaga taaagaagtg agaaatggaa
    12721 aatcagtgaa gagtgaggcg ttcttgcggc tgtagatcgt attctacaaa aaaatcgcct
    12781 tgtgcgatac cttgattcct cacttctcat tcctcaatca aaaaaatttt cgttgacatc
    12841 aaaagaaaaa tacgctataa taaaagagaa cggaatgtcc gtaaaatttt atgatgggag
    12901 taatttcgat gaaaataatg gatactatcg gctttgttga aaatgtcgat aaagaagtcg
    12961 gcgaagcaat ggaagccgag ctggcaagac agagaagaaa tcttgagctt atagcaagcg
    13021 aaaatatcgt ttcacctgcc gttatggctg caatgggttc tgtgcttact aataagtatg
    13081 ctgagggata ttctggtaag agatattatg gcggctgtca gtgcgttgac gttgtggagg
    13141 atatagctgt tgaaagagcc tgcaagcttt tcggtgctaa gtatgctaac gttcagccac
    13201 attccggcgc tcaggctaac actgctgttt attttgctct tcttaagccg ggcgacactg
    13261 ttatgggtat gagccttgac gctggcggac accttacaca cggctcacct gtaaaccttt
    13321 caggtaagta ttttaatttt gttccttacg gcgtaaatga tgagggctac atcgactacg
    13381 aggctcttta caagcaggct aacaagtgca gacctagact tattgttgcc ggtgcaagtg
    13441 cttatccaag agctatcgac tttgagaagc tttctgagat agcaaaggct gttggtgcat
    13501 atcttatggt agatatggcg catattgcag gacttgttgc cgcaggctgt catcagtcac
    13561 ctgtaccata tgctgacatt gttacaacaa caacacataa gaccctgaga ggtccaagag
    13621 gtggacttat actcacaaac aacgagtata tagctaagaa gataaattct gctattttcc
    13681 caggcacaca gggcggtcct cttatgcacg ttatcgctgc aaaggcagtt tgctttggcg
    13741 aggctctcaa gcctgagttc aaggcatacg gtgagcagat cgtaaagaat gcacaggctc
    13801 ttgcaaaggg acttcttgac agaggattca acctcgtatc aggcggcaca gacaatcacc
    13861 ttatgcttgt tgacctcaga cctttccaca tcacaggcaa agagtttgag aaaaagcttg
    13921 acgaggttta tatcactgta aacaaaaatg ctatccctaa cgaccctgag aagccatttg
    13981 taaccagcgg tgtaagagtt ggtactgcgg cagttaccac aaggggactt gttgaggagg
    14041 acatggacgt tattgcagag tgtatgtatc tcacagcaac agactttgac aataaggctg
    14101 atgagatcag agaaaaggta aacgctatct gcaagaaata tccactttac gaataattgt
    14161 acggatttat atactgttga tacacatagg gagcggctta ggtcgctccc tttttctcaa
    14221 taatatatga ataaatctct cttttggttg atttttaaca gagatcgtgt tataataatc
    14281 ttttagagta taattaaaag gtgggatata ctttgccaat taaaatacca aatgaccttc
    14341 ctgcgttcca aacgttggaa aaggagaata tattcgttat tcccgatgac agagccgctc
    14401 atcaggacat tcgtgccttg aagatcgcta ttgtcaatct catgcctgac aaaattgcga
    14461 cagaaacgca gcttctgcgt cttttgagca acacacctct tcatgttgac atcgacctta
    14521 ttcagatgag cagtcatatg tcaaaaaaca cctctttgga gcatatgctc gctttctaca
    14581 agaactttga cgaggtgtgt gataatcgtt atgatggtct tataatcacc ggtgcgcctg
    14641 ttgagcaaat ggagtatgaa gacgttgatt actgggacga gctttgcaga gtttttgaat
    14701 ggtcaaagac caatgttttc tcaactatcc acatatgctg gggcgctcag gccggacttt
    14761 attnnnnnnn nnngttccca aatatccttt gaaagagaag atgtttggca atttcaagca
    14821 ccatatcgac tatcccgaaa gcattattct taaaggcttt ggagatgttt tccattgtcc
    14881 gcactcacgc catactgagg tgcgtcgtga agacatcgaa aaggtggacg accttatagt
    14941 ggtggcagtg tcaaacgagg cgggtgtgca tcttgtttca gataagacac aaagacagtt
    15001 tttcctgttc ggccactggg aatatgatcg tgagaccctt gcaaaagagt atttcagaga
    15061 taaggaaaag ggactgccta tacagatacc atatagctat tttcctgacg atgatccgga
    15121 aaacaagcct attttcaatt ggagctgttc ggctaatctt ctttattcaa actggctcaa
    15181 ctactgcatt tatcagaaaa cgccatatga tcttaacgaa cttgagccac tgggggctgc
    15241 acaatgaaga acggcagaca tatagctgag ttttacggcg ttggagtaat gcttgcaata
    15301 gtgggtggct atcttgacgc ttatacctac atcagtcgtg accacgtttt tgcaaatgca
    15361 cagacaggta acatggtgtt gttggctatc aatatcaaag agggaagctg gctgaaggtc
    15421 tttctttatc tcatgcctat actgtccttt atgcttggcg tgctgatagt ggaaactgtg
    15481 aagcttaaat tcaatgaaca cccgaggata cattggcata ggatcataat cggtgtggag
    15541 cttgtggtgc tgactattgt gggatttatc cctgctggta cgcttaacac attggcgaat
    15601 atcctagttt cttttacctg tgcaatgcag gtcgagggtt ttaggaaaat ggacggaaag
    15661 cctttcgcta ccacgatgtg tactggcaat cttagaagcg gtacggataa tctgtttcag
    15721 tatttgaaaa ctaaggataa gcaaaagctt ggtaatgcaa tgcagtatta tggtataatc
    15781 ctttgcttta taggtggagc ggcagtgggt gcatttgtca cagagttgct tgaaactaaa
    15841 gcggtgtttg ttgctttgac aggtcttgtt atcgcactct gcattttaag aagcgatgac
    15901 gataaattgg cagaaggtaa gtgacaatca ccgagtgaag ccttgtcagg gcttgaggca
    15961 gagactgaat atgcgtaagc cagacagtaa aataagaaaa tcaaaaaaag caaagcagtg
    16021 tttgagaaaa atctaagcac tgctttttct atttcatttt gcaaaaccat atttcaaagc
    16081 acaaagtgaa ttggtggcaa aacaaagtaa tatggatatc caaataataa ttgcaaaaga
    16141 tataacgtca caaaagcgaa aatgatgtta tctgactgct tttgattgaa tgttgttaaa
    16201 atgcacaaat atcttattca taatttataa aaacggccaa aaataatgtt gtgtgaatga
    16261 ttttgaacgg aattgcttga cttttgctgt acgatatgtt attttaatat cagaaacaat
    16321 caatatcgtt caattgacgt caaaaaacat tattgaaatt tgaaaaggtg ataaaatgct
    16381 tacagaggaa aggtactcta ttatattaga gagagtcaag cagaataaaa gtgtgaagct
    16441 tttggagttg tgcgagcttt taaaagcttc tgaatcaacg atacgccgtg acttgacggt
    16501 gcttgatgaa aggggtcttt tgaaaaaagt tcatggtggt gctatatcag ctgaggaaca
    16561 ttctttcaat ccggaggaac aggacgttga gtcaaagtca aagcttttta ccgatgaaaa
    16621 gcttgcaatt gcgaggtttt cagcatctct tgtggatgac ggcgatttca tttttatcga
    16681 cgccggaacg actacaggct atatgataga ctttctgcca gataaaaaaa ttacctttgt
    16741 gactaacgct ttcgttcatg ccaaaaagct tgctcagcgt ggctttaagg tctatatgcc
    16801 ggcaggggag ataaagctca caacggaggc tatcgttggt gctgaatgtg tcagcagtct
    16861 gcaaagctac aatttcacca aaagctttct tggagcaaac ggaatttcgt tatcaggtgg
    16921 tataacaaca ccggacagaa acgaggcaag agtgaagtct accgtggtgt caaacagtca
    16981 gactgtttat attcttgcag atcactctaa attcgatcag atatctgcgg tcactttcgc
    17041 tcagcttgga cgtgtgaaaa taatcaccga caagcttcaa gacaaaaaat atcttacaaa
    17101 agcaaatatc aaggaggtaa tgtaaaatgg tatatactgt aacattcaat cctgctatcg
    17161 actatgttgt tcatacaggg gaaatgaagc ttgggtctac caaccgctca gatagggaag
    17221 agatgtattt cggtggcaag ggtataaatg tgtctgtagt tcttagagaa ctggacattc
    17281 agtcaaaggc acttggcttc actgcaggct tcacaggtga agctatcgag aacggactag
    17341 cggaaatggg aattgataca gattttgttc atctcaaaaa cggtaattcc cgtataaatg
    17401 tgaaaataaa atctgctgag gaaacagagc tgaatggtca gggccctgat atagatgatg
    17461 aggctgtgaa taagcttttt gcaaagcttg ataagattca ggacggagat acgcttatac
    17521 tcgcaggaag catacctgcc tcgctgcctt cagatatcta tgagaagata ctcgaaaggc
    17581 tcgcgcacaa gaatatcaga acagtagtgg acgcaacaaa agacctgctt ctcaatgtgc
    17641 ttaaatataa acctttcctt atcaagccta acaaccacga gcttggcgag atgttcggag
    17701 tgacactcac taatgatgag gagatagaaa aatacgcacg taagcttaaa gaaatgggtg
    17761 ctgtgaacgt gcttatctca atggcagggg acggcgcaat gcttattgac gaaaatggtg
    17821 aaatacatcg ctgtggagta tgtaacggta aggtgaaaaa ttccgttggt gcaggcgatt
    17881 caatggtggc aggctttacc gccggtatat tgcagggcga ttatgaatat gctcttaaac
    17941 ttggcacagc cgctggcggt gcgactgctt tttctgatgg gcttgctcaa aaagaaaaaa
    18001 tcgcagagct tcttgaaacg ctatgaaaaa atataggaac gcggagcaat ggcagtttca
    18061 ggtgcaactt ttgctgctcc tgtcggaaat gttccatcag gctttcttgg cgcattgcct
    18121 gcaggtttcc ttaggaaagt tacagaatag tctttacaaa tataagaaaa tataatataa
    18181 taatgttaac aatcataaat aaattaagtt tcagaaaagg agggatttat tatggtatca
    18241 aaggtcgtaa aagtagtaaa tgcagaggga cttcatatgc gtccggcagg tatcgttgca
    18301 aaggctgcta aggagcatcc tgagtgcgag atcatgatga aagcaaacgg caagaacatc
    18361 aaggctaagg ctgttatgca gataatggca gcaggtatca agaaggacac tgaggtagag
    18421 ataatcgtaa caggcgaaaa cgagcaggct gtgcttgacg agttcgttaa aatgtttgag
    18481 gacggtttcg gcgaataatt ccttatatct ccaaaaaaaa ttcagacaag accgtagcgt
    18541 aaactgcggt ctttctgagt aatatgacgt tgtaaaaaat ccaaaagaga gaaatacata
    18601 tggacaaaaa atatctttta cttcttgcac aaaaatttcc tactatagac agtgccgcca
    18661 gcgagatagt aaatctatct gcaatacgca gtatgccaaa gggtacggaa tactttttca
    18721 gcgatatcca cggggagtat gaagcctttt tgcatatgct aaaaagcgca tctggcatga
    18781 taaaaaacaa gatagacctg accttaggaa aagcagtttc ctcggcagag cgtgaagccc
    18841 ttgcctatct tatctattat cccgacaagc agctaaagcg tctgcgtgat aaaaacgggc
    18901 tttcagacga gtggcgcaga cttactatct atcgtctgat acttgtttgc gaggcagtgt
    18961 cagcaaagta tactcgttcg agagtgcgaa agagcatacc aaaggatatg gtctatatac
    19021 ttgacgagct gcttaatgtc actgatgatg tggtaaagga atattactat gacgagataa
    19081 tcaccacaat tcttgacact ggaatcgcag accgctttat aaagtccctt tgtgagcttg
    19141 tacaatctct tgctatcgac aagctgcata ttatcggaga tatatatgac cgtggtccaa
    19201 gagcagatat tattatggac gagcttatga aaatgcacga tgtcgatata cagtggggca
    19261 atcatgacat ttcgtggatg ggtgcggctt caggtaatct cgctcttatt gcaaatgtta
    19321 tccgtatctc catgcgatac aataactttg acgttctcga ggacggctat ggattaaatc
    19381 ttcgtgcgct ggcggtcttt gcaggagaaa catataaaaa cgatgagtgt gaaattttca
    19441 tgccacagac acttgatgat aatatctatg accctgttga tgcaaagctt gtggcaaaaa
    19501 tgcacaaggc tatcacagtt atacagctaa agctagaggg gcagcttatc aggcggcacc
    19561 ctgaatggaa actatctcac cgtgaccttt tcagcatggt ggacttcgca aatggcacta
    19621 taacaataga cgggcaaact cataagcttc ttgacggtaa ttttcctaca gttgatcctg
    19681 ccgatcctct tgcgctcacc gaaggcgaaa atgagcttat gacgattctt gcaaactcgt
    19741 ttatgcacag cgacaggctc aacacacata tgcgtttttt atattcaaaa ggctcaatgt
    19801 ataagactat aaacggcaat ttgcttttcc acggctgtat ccctcttgat gaaaatggaa
    19861 agcttctgag cttaagtata gcaggagagc agtattcggg taaggcaatg ttagacaagc
    19921 ttgacgaaat agccaacaag gcgtatttct tacagccttg tgaggagaaa agcaactgtg
    19981 cagaccattt gtggtatctg tggagcggtg cacgttcgcc cctttatggc aaggacaaaa
    20041 tggcgttttt tgagcgatat ttccttgaag ataaaaagct tcacaaggaa aattataatg
    20101 cctattatca cttttcagag aagcctgaga tatgcggaaa gatacttgaa atgtttgggc
    20161 ttgacccaca gcgtggtcat atcataaacg gccatgtgcc tgtgaagata aaaaatggtg
    20221 aaagccctgt caaggcagac ggcaggctgt tcgttattga cggtggtatc tcaaaggctt
    20281 atcaaaaagc caccggtatc gcaggctaca ctcttattta tgattcccac agccttaatc
    20341 ttgcggagca taagccgttt attgcaggag agagtgagca tactcctgag atacatatgg
    20401 tggaaagact tgaccgccgg gcaaacattt cagataccga caagggaagg gaactgcttt
    20461 tgcagataaa cgatctccgt gacctgctgg acgcttatcg ctctggagat atcaaagaag
    20521 gttataaggt attttagacg ttctgaaagg agcttaacat atggaaaagg gagaactgaa
    20581 ctgggctgtt atcggctgtg gcgttatcgc aaacgaaatg acaaagctta ggaaagaatg
    20641 gggacttaaa tatcctaagg agatatggtg atatccacat aatgatataa caaaccaaaa
    20701 ggcactgact tcaattggaa gccaatgcct tttttatctc ttgttttgct gtgaaaacaa
    20761 gtacaaatta tagtacaaat aaattacaag tacaaaaata tgtacgttta tcacacgaat
    20821 tcagagcata tatttttgta acattcattg gtggaataga cccattttgt gagcctgccg
    20881 ctggtgatga aatagtttat ctttgaggga aaaacgctgt tttcaaatac gtctacgata
    20941 atgagcttga ctttcatttt ttcggggata agagctttta tgtaaacact tgctgcctgc
    21001 cccacacgca cattttctct tggttcagca agtcctgcaa ggttcggagc aagctctata
    21061 aaaataccgt atggttcaat ggagcgtact atcccaccta cagtttctcc tgtggagaac
    21121 aatgctgcat tttcttccca tgtgcctaaa agttccttgt gagaaaggca tatcctgtcc
    21181 tcgtcacagc tttttaccac agcatatatg tcctgaccgt tgaaaaatct gtcgttgggg
    21241 tgggtgatgc gtgagacgga tatggcgtct attggtataa gtgatggaag accacagcct
    21301 atgtcaacaa agcacccgaa aggctcaagg tgtgttacct ttgccggtat aacgtcccct
    21361 gatgtgaggg tacttatata tcgttcaatg cactcttctt gtgcaagacg ccttgacaac
    21421 ctgcatatga cctcgccctt ttcgtgaaca atatctgtca ccttgaagca taccgttttg
    21481 tttaccctag aaagtaatgc tatgtctttt gttttgcctt cttgtattcc gattgctccc
    21541 tctgatcttg ggattattcc tttgccaaag ggaagctcta ctattatgtt gtgttcgctg
    21601 tcacaaagca cagcatgacc ctccagtatc aatccctgag ccattgcctc agccattgtt
    21661 cgttcgcttt gaagataata tcggttttca gggctattga agatcttgcc ctcaggtaaa
    21721 tattttttca ttattttgcc tctttcccca cagatttttt taaaacagca ggatctctgt
    21781 ggttgatcca ttgattttgt tcatattctg cacatatttt attt
//
LOCUS       DAWEMR010000019        21291 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1038_length_21291_cov_10.912978,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000019 DAWEMR010000000
VERSION     DAWEMR010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 21291)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21291)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21291
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1038_length_21291_cov_10.912978"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3339..3348
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6513 a         4391 c         4981 g         5396 t
ORIGIN      
        1 tgttgcgcgt ttcttaaaac atagtacaaa aagcaagaaa attaagtaaa aaacttgaaa
       61 ttatatcgta aaaatgttat actgcaagtg tagtataaag ttaaaaaaat aaaaaaaccg
      121 gaggaagatt taaaatgtgt aaaaaagacg tgatcaaaat ccttgtcgga agcctcaatc
      181 aggccggcat gaaggtggca gactatctga aaaaacaggg gtacaatgtt gcgcagtcag
      241 actacaatcc actggagata cagcttgagg tgcttaaaag caaacctgat tttctgatag
      301 tttcagaaat aactgagttt ccctacgaac tgtgcaaaaa tctcaaaggg gcttgtccta
      361 agctgaaaat aatcatgctt tccgacagaa aaaccaacaa tggaaacaat ggacttgcaa
      421 aatacgcaga catgataata aaaagtcctc taagctgtgc ggatatcaaa aacgcactca
      481 gagcgcttca gtgtcctgac gattctgcag atctgaacat cacaataaca cgaagctctg
      541 acgaggggta caccaatggc gacatacaaa ctgctatatc caccgtgatg aagcggttgt
      601 gcataactcc aaattataat ggctatggct acatcaaaga ggcaataaaa atggctctta
      661 cgacagacaa agccaacaga cgtgtcacaa aggtcattta tccagagata gcggcggcac
      721 atggagtaac cacagtaagc gttgaaagaa acatgcgcac cgccataagg atcggttggg
      781 aaaaggctca cgacaaagac aaagccgaga tattcggtaa tttcgccata agacctgacc
      841 actaccccac gaacagcgaa tttattttcg ttgccgccga atacataaac agcaaatatc
      901 cagcggaaaa ggcattatca gccaccgctg aacatactag cgaaagtaat ttatttatct
      961 gacaagaaga aagccgttat gcccacaggc acagcggctt ttttctatat aaacgaaacg
     1021 tcctgcagta aatgacaacg ggctgccctg cggacgcaga tcttcggctg cgtcacccac
     1081 tgtcatacga tcactgcaga cgctccgtta tctcatttta tgccgacatg gtttttatga
     1141 cacaaaaaaa gcgtaccgaa aaatcggtac gctcagtgtt taagtaaaag ccgccttgcc
     1201 gtcgcacagc cgataaaccc gctataaaag cagggtgggt aaaacagcct gaaagcttca
     1261 cgctcccttc gtccgtttcc gaaacggcaa atgcggtttc gttcgggagg tctgcaatcc
     1321 atttccagag tctgaatccc tttttagaag tgttggattt ataataactg tgcttgttcg
     1381 cacaaggcag gaaacttttt ctgttattta gtatatcaca acatatccat aatatcaata
     1441 ggtaatttca gcaaatcaaa gtacataaaa ttgtacattt tacatattga caagcctata
     1501 tgctgagata tttttcttat gacaggtgat cgtgacaacc gcagccacag ccgcaatcat
     1561 cgtcttcgtc ctcatcgtca aactcgaaca tttcatcgat acgctccatg aacatctgac
     1621 cgatcttctc atactcgtca tcatcttcga tagaagcaag tatctcgtca ttgttctcgt
     1681 catactcagc cttgagtatc acaacgtcgc ctgaatcttc aagggcttcg tcaccgttct
     1741 cataataagg agtgagagca taatacttct cgtcaccata ttccattgcg tccaacagct
     1801 caaaagtctg cttattacca ttttcgtcct ccagctcgta gaggtccggc agaaactcgt
     1861 tatccataat tatcctccta acatgatctt gtgcggtata tcggcacttt ctgagtggga
     1921 tatatcgaaa aaatcagaac agaaagactg tttctgacca ccgcaacact ttaattatac
     1981 tacactgcaa aatcaaagtc aagcgcattt tgtaaaagta atattaaata taaaaaccac
     2041 aaaaaagttg agaaaaacta ttgacaaata acaaaatatg tggtatagta tagtcaagga
     2101 aaaacaaaga gccaaacaaa agcagacaac agggttcgct gaaacggtca acggtctgac
     2161 gatacagtaa cgatttcaga gggcacggca aagcgaagtg aggcaagagg ttttccttat
     2221 atatcacagg cagatcggtg gacacagcat atacccgaca ctaggtacac tacagttcag
     2281 caggcgggca ggctcatcgg atacctaata agatatgaaa ggagcgagtc caatggacgc
     2341 atcagagatc caggagagta tccataggct cggcaatttc ttaaagtaaa aaaggatcaa
     2401 gacgcagacg aatgagctga caggaatcca gacatatatg atttacacag gaagaaattg
     2461 cagagcctca tggattaatc acagcacaga gttactgcaa tttaaggata ctctcaaatt
     2521 tacaattgaa tattttgcaa cttttatata aggagtgatc gacatgatcg gttacagaag
     2581 gtttgcaaat aaaatttaga caggagtgtt tccaatgcac aatttcagct tttcagaggt
     2641 aaagaaataa actgaaagag tgattccaat gtaaaattta gcttctgaac ataaaaagct
     2701 cacagagcta taaaaatcaa ataaatttcg aggagtggtg catatgaaaa tgcacaaaag
     2761 gaacttgaac atgaaataga atggagtgag tccgccaaga aatttacgag cttaatgctc
     2821 ttactgattt gatttactga ctaagatttt tctaaagatc atataaaata ctgaaggagt
     2881 ggtgcgttat gaaaatgcac tcaaggcttt tgaacatctg atatttggag gtgaatccgc
     2941 cagttacttc agcaaggctt atctgatatg atgagcatag gatcttgaaa ttatgcgaaa
     3001 ggcgataatt ccaatggaaa tgtaataagt ttcattgaca ataaattttc acgaaaggtg
     3061 ttaagtccga tggcacgtta agttttatcc actgaggcat aacttaatat tttgaaagga
     3121 aataaatcca atggaaaggt aatttgcact tagagattat ttttaccgaa aggaaataga
     3181 tccaatggaa tagcttatat tatgcggaca gaattttact gaaaggcgtg agtccaaagt
     3241 acaatttagc atgagtttac gactagaatt ttcagaaggg cgtaatacca atggcataat
     3301 caatatgaac gtacatactt aactatgtgg tgtattcann nnnnnnnnat cacatagtta
     3361 agtttttttg tgtaaggggt gggacgtcga gaacgccgtc ccatacaacg tatcaagaat
     3421 aaataaagca ccctgcggtc aatgcagagt gcttttattt tatatcatat tttcaaggaa
     3481 agcaactgtc tttggatcgt atgtaagatc ctgcgcttcc atatcctcgt caagctgcca
     3541 cagctttgtt actgcgaaaa ctgacgatac aaaaaacggc tggctgttca gatagccgag
     3601 ggttatcttt gccggagtgt cccccgtctg ctcgctgagc tgttttacct ttgcaagtct
     3661 tgcaagatta gcaggacctg catactgtcc ctcagggaga ttgtttgcag cgtcgccctt
     3721 tgcaagcttt gcaaaaaagc cctttgcctg cggggagaac gccataacag gaaaatcatg
     3781 cttcttgtac cagcggaact cctttgtatc catacaaaca agagtattgt caccgaaagt
     3841 ttcaacgctc gaatgagcaa ggctgtagtt tatctgactg atcctgatag gctccatacc
     3901 gtttttctct gcaaacttat tcgcttcctc tatcctctga gttgtccagt ttgaaacgcc
     3961 tataaaatgt atctttccgc tctcataaaa atcattgaga actggcatta tctcttcaac
     4021 aggtatcttc gggtcgtcac gatgaagaaa ataaatatcc acatagtcaa cgcccagcac
     4081 tctcaggctt gcttcaagat cctttgtaag ctcttccctg ctaagacggc tttcgtccat
     4141 atgacccatt tcagggtgac cgccctttgt ggcaatacaa attttatcac gattgtttct
     4201 ggctttgagc catcttccta ttacgccctc gctggaatcg tgaccattct ccagccagtc
     4261 gcagtaaact cttgcggtat cgatacacca gccgccaagc tctacatatt tgtcgagaaa
     4321 ctcaaaatac acatcgtcac aatcgttcct ctcaaaacct gccgcaccaa aagtcagggc
     4381 agggactttc atttcatatc tgtcgttttt caacacatag cttatcatgt actcttcccc
     4441 cttggcatac aatttcctat actatttatc atcactatgt ctagcaatta attattatac
     4501 catataaaaa ggactttttc cacagaattt tgttaaattt tattttttca taaaaacccc
     4561 ttgacaagtc ggtaaacttg atatataatt gtaaaggttt atgactttac agaaatccat
     4621 taggaggtaa gcggtatgag taaaaatttg caaaacagcg ttttgctcga cttgtatggc
     4681 tgcctgctca ctgataaaca aaacgagatc atggatcttt attacaatga cgatctgtcg
     4741 ttgggagaga tcgccgctca gctagatatc tcaagacagg gcgttcatga cgctataaaa
     4801 aaatgtgaaa ctgcaatggc tgattatgaa gaaaagctag gtctgctaaa acagcaggag
     4861 gcttatctgg cagagcttaa agagttcaag gctcaagcta tggacgtttt taacgagtgt
     4921 aaaaaaaaga attatgcgag agatattgcc gaaaaggtga tagtgcttct tgaaaatctc
     4981 gattctaagc ttgatgaatt tgacaacgca gaatataccg aagaagaata gatatagata
     5041 aaaaaattat acggaggaca gcttatggcg tttgaaggac tttcagaaaa actaaacggc
     5101 gtgttcaagc gtcttaaatc aagaggtaaa cttagcgaag ccgatgtaaa agaggcaatg
     5161 cgtgaagtta aaatggctct gcttgaggct gacgttagct acaaggtagt aaaggatttt
     5221 attgccaagg taacagaaag aagcgttggc gaggaagtgc tgaagagcct tactcctggt
     5281 cagcaggtca taaagatagt taatgacgag cttataaagc tcatgggcga ggctaactcg
     5341 aagataaact ttcctaacaa gccgccttgc atcataatga tgtgcggtct tcagggttca
     5401 ggtaaaacaa cgcacgctgc aaagcttgcg aagatgttca agaaagaggg caagcgtcct
     5461 ctgcttatcg cagccgacgt ttacagacct gcggctatcg aacagctaaa ggttgtggga
     5521 gagagagccg aagtcgcagt atttgaaatg ggtcagatag acccacgaaa gatagtaaaa
     5581 gagggtatca agcatgccaa ggattacggc aatgaccttg ttatcataga tacggcagga
     5641 cgtcttcaca ttgatgagga gcttatgaac gagcttaaag acatcaagaa gatcgctgag
     5701 ccgaacgaga taatgctcgt tgttgacgcc atgatcggtc aggacgctgt aaaggtagcg
     5761 tcaagctttg acgaggctct tggtatcgac agcgttatcc ttacaaagct cgactctgac
     5821 acaagaggcg gtgcggcgct ttcagtgctt gctgtaacag gtaagcctat caagttcgtt
     5881 ggtatgggcg aaaagcttga cgagtttgag gctttccacc ctgagagaat ggcttcaaga
     5941 atacttggca tgggcgatat gctcacactt atcgaaaagg ctactcagac agttgacgaa
     6001 aaggacgcca agaagcttgc agaaaaaatg caggaaaagg gctttgacct taacgacttg
     6061 ctggagcaga tgaagcagat acagaaaatg ggctctatga agtctattat cagaatgctt
     6121 ccaggcgcaa acaaggtaac agacgagcag gtggagcagg gcgaggtcca gctgaaaaaa
     6181 actgaggcta tgataaattc aatgacaagg gctgaaagag aaaagcctgc gatcattgac
     6241 cctaagagaa aaagacgtat cgctgccgga agcggcacac aggtatctga cgtaaatcag
     6301 cttcttaagc agtttgaaca gatgcagaaa atgatgaagc agttcggtat cggcggaaat
     6361 cttcacggta agaagtcaag acgaaacagg gctgctcttc tcaaaggtct tggcggcgga
     6421 atgccacaat agttgataag ctatcagcaa tcaagtgatt gatttgatat atgaagtttt
     6481 ttatggaggt gaaaatacaa tggcagtaaa gatcagactc agaagaatgg gtgctaagaa
     6541 ggctcctttc tacagaatcg tagttgctga ttcaagatat ccaagagacg gacgtttcat
     6601 cgaagagctg ggctactatg acccaactaa ggaaccttct gtactcaagg ttgacgacga
     6661 aaaggcaaag tcttggatcg ctaacggtgc acagccaact gatacagtta aggctcttct
     6721 caagaagaac ggcactatct aatagctgac aagacaattc agacggtgag ccacctatcc
     6781 caccacttcg cttgccgttt tcggcgggga aaacgcttcc ctgccgcatt ttcgtgggag
     6841 aatggagata tttttatgaa agaattactc gaaactatag tttctgagct tgttgaaaac
     6901 aaggacgctg ttgaagtcgt tgttgacgag cctaacgcag agggtatcgt ggtttatcat
     6961 cttcacgttg ctccggacga tatgggcaga gttatcggca agcagggcag gattgcaaag
     7021 gctataagag ttgtaatgcg cgccggtgct tcccgtgtgg atcagaaaat aatggtagag
     7081 atagactgat agttcagtga ataaatcatc accccgtaca gataatagta tggggtgatt
     7141 tttttgcgta taaataaaaa atgcttcaat gcgctcacag gtatagccgt ggggatatgc
     7201 aacggttttt tcggttcagg tggaggaatg atagcagtgc ctatgcttga aaaaggcggc
     7261 agcgaggcga aaaaagctca cgccacgtca attgctataa cgcttccatt gtctattata
     7321 agcggacttg tatatttcaa gggcggcagt cttgattttt cgcaggcagt gaaattcata
     7381 ccacttggga tagttggtgc ggcagtgggt gcaatgctca tgaaaaagct ttcaaacagc
     7441 ctgctaaaac gcattttcgg cgcacttatg ataattgctg gcgtgaggat atttatgaaa
     7501 tagggtgata ttacggcttg gtatacaata tgtgcggtgg cttttctcac cggtatcttt
     7561 gcctccatgg gtcttggggg aggaatggtg cttatagttt atctgacggt ctttgcaggc
     7621 ttttctcaga tagcggcaca ggggataaac cttgtgtttt ttatccctat cgcaataata
     7681 agccttgtgc ttcacacgaa aaacaagctt gtggaatgga aaaaagccgt tcccgcagtt
     7741 ttgtggggaa cggccgctgt tattatatca gcatggcttg caaacaggat agagcaaagc
     7801 ctgctttcaa aagcttttgg tattttcctt atcctaatgg gactgaaaga gttatttttc
     7861 aagagtgaaa agcacaagtt caaaagggtc tgaaaaacgc tgtgtccagc cactattgcc
     7921 tactcccctt gacaccacaa gcgttgtatc gttcacgctg taagtgccgt cggtgtattt
     7981 cgggaacaga ccctgaccgg gtgcataaag accattcaca agtggaagcc ttataagtcc
     8041 gccatgtgcg tggcctgtga agtctatgtc tatatgcgtt ccatactgca ctgcggtctt
     8101 gctgaaatag ttgcaatcct cgggatagtg atttagcagt atgacaaaat cctctgtgac
     8161 atgagaaaaa gcctcgctgt cattttccat attgtcaaag cccaacaggc ttatagtatc
     8221 accatttcgt gtgagtgtgg tatgctcgtt atagaggacg tttacaccat agctttcaca
     8281 acctgcaaca aactgttccc tttcctcctg agaaagattg tattcatggt tgcccataac
     8341 aagatagaca ggacatatgt caggaagctt tttcataaac tcaagggagc ggtcaacatc
     8401 ggggaagatc tgatctacgc tgtcccctgt gacaaagata tagtcaggct cacagtcttt
     8461 gaccttttcc gtcagcctat ctatgtagcc cttgccccac tcgttgtgga agtcagatat
     8521 ctgaactatc ttacagccct caaagccatc aggcaggttg tcaaaatgcg ctgacagctt
     8581 tttgacctcg atataggtat tagaccacac gaaatcagct aataccatta ccacaagcac
     8641 tattatcact atgatgatat ttcttttctg ctttttagtc agcctgcttt gctttttttc
     8701 ttctgtattc aagatagccc tccaaaatta tcgttgcggc aactgtatct acaacagcct
     8761 ttcttgcctt tcccctgacg tttgtttcgt taaggatatt gtgtgcggtg acggtagtgg
     8821 aacgctcgtc ccacattctc accggcaggt cggtacgctc tttcaaaagc tcggcaaatg
     8881 ccctgcactt ctctgctctt gggccttccg tgccgttcat gtttataggc agacctacta
     8941 ttatctcacc tgcaccgtat tcagccgccg tctgagccgc cttatctgca agcctttcgg
     9001 cgtttctctc aggaacagtg ccaataggag aagcaagaaa ctcagagcgg tcgcttattg
     9061 cgaagcctgt tcttgcgtca ccataatcta ttgccattat tttcattttg cacacctcta
     9121 tatcaagact tttcttatca ttatatcata acactgtctg taaaacaagt cctaaagcag
     9181 gattttggga attaaactgc aaaaagcgca atgtagaaga gctctctgca taccatttgc
     9241 ttaaaaaagt aaaatagttc cttaaatact gtgattattt gtgcattatg gttattgatt
     9301 tttattttta attatgttat aatgctttat tatcagaggg tatgcacaaa tatcagggct
     9361 aagttttgtg caaaattata ttatataaga attggaaagg aaaaagaaaa atgaagaaat
     9421 ttttatcagt agcaatgtct gctatcatcg cctgcgcttc aatattctca tgcacactga
     9481 cagcttttgc cgaaaacgca gaaactgagg acgttactat cgactgctct tccgcagagg
     9541 cttgtaacaa ttggagtcag tctatcactg tcgatcaggc aactttcaac gcaacaagac
     9601 ttacaaagga ttcagagata atcgtcacat tcaagagcga ggaaataaac gaaaaggcag
     9661 ggaacaaata caatgctgag cttattttcc agagctggga caacaccact acaccagccg
     9721 ctcaggacgg tgcggtatgg gcaaagatag cacctgtgaa gtttgacgat tcatcagcta
     9781 catatgattt tgaatctatc gcaactgcat acggcacgga cgatttcagt caggtataca
     9841 acatcataat cggtgcgact gacagggcaa agataacagt aacaggcata acggtaacga
     9901 actgcaagac aaagacttat gcagaaaagg aagaaaaaga ctcaaaggga acaaacccta
     9961 ttatcatagt tattgcggtc atcgcaggaa ttgctatcgc agttgtggta attgtgataa
    10021 tcatgaacaa gaagtcaagc gaagcctttg atgtaagcac cggcaagttc gttgacaaga
    10081 aaaatctttt tgacgagcct aagaacgacg aggacgagaa gaaataaaaa ataacgctat
    10141 cagcaggagg acgagcagtt cgttctcctg ctttttttcg tcaaaatacg tcgcaaaatt
    10201 gcttgacaac atggtttgat gtggtataat aaaagcaaga acgaaacaat tagtcagata
    10261 tttaaggagg cagagctatg agtacaaaag aacttgcata cagtatgata gacaatctca
    10321 cagaagaaca gctcaatgct cttattgtta tcttgcaagg acttcagaaa gaagagccta
    10381 acgaagaaac aaagaaagtc cttgacgaag ccaaagaggg taaaaatctt gttggtccgt
    10441 ttgattcggt agacgagctt tgggagagtc tgaatgctta ggataactta tcacaacaaa
    10501 ttcaaaaaag acttcaaaaa agcaaaaagc cgtggccaaa atatgtcgca ccttaacgaa
    10561 gtcattacca tgctagctga ggaacagcca cttccggcaa aatacagaga acattcactg
    10621 accggcaatt acaagggttt ccttgaatgt catgttgagc ctgactggct tttgatctat
    10681 gaaagatgta gcgacgagct tgtactgctt ttatacagaa caggaactca cagcgatctg
    10741 ttctgatagg tcataggata ttttttaaca tcacagcagg aggacgagca gttcgttctc
    10801 ctgctttttt tataccagaa atattgactt atagacggag ttatggtatt atattaaaag
    10861 gtatatttaa taattggagg ctatataaaa tgtcaatatt taaacttgat ccagcattta
    10921 aggactacat ctggggcggc accagactta gagatgagta cggcaaaaag tgcgattatg
    10981 ataaggtcgc agagagctgg gagctttctt gtcataagga cggtccaagc gttgtggctg
    11041 acggtgaata caagggtctg acactcaatg agtatatcga aaaggcaggc aagggcgttc
    11101 ttggcaagaa ctgcgaaaga tttgagaact tccctatcct tattaagctc attgacgcta
    11161 aggacaatct ttctgtacag gtacaccctg acaacgagta cgcaatgaga gttgagggcg
    11221 aatacggcaa gacagaaatg tggtatgttg ttgactgtga cgagggtgca actcttctct
    11281 acggtttcaa gcacgagata acaaaggaag aatttgcaag aaggatcgct gacaacacgc
    11341 ttcttgaagt aacaaacgct gtacctgtta agaagggcga cgttttcttc atcaagtcag
    11401 gcacgctcca tgctatcggc aagggtatcc ttattgctga gatacagcag aactcaaaca
    11461 ctacatacag aatttatgac tatggcagag ttggcaagga cggcaagcct agagagcttc
    11521 acgttaacaa ggctcttgac gtaacaaagc ttgctcctgc tgagcagtat cctgagacac
    11581 ctgttgagaa gaaggatggc tatgacataa agcttctttc aaaatgcgaa tacttcacaa
    11641 catacagggt gaacgttgaa acaaaggctg agcttgacgc tgacgaaaac agcttcaaca
    11701 gtattctcgt tctcgagggc gagcctgtta tcagcggtgg cgaaactgtt tctgcaaaga
    11761 agggcgaaag cgtattcatt tcagcaggca caggcaagta tactgttgag ggcaaatgca
    11821 cattcgtgct tacaaagata gactaaggtg agataaatgc ttaaacttga cctgaaaaag
    11881 gagataggtg agcttggcgc acttgtgatt gcttttatta tcggagttgt ggcaagctca
    11941 ttttggatag gtcttatcag ctttgtagtg ctggagcttg tgtatcttct ggtatatgac
    12001 gccatagcaa aaaaggctga agcccaaaag ccaaaagagg aaaaggaaga gataaacttt
    12061 cacgaatatg atgatgacac tgagaacgat aacgctcagt aagctcagaa aataaaaggg
    12121 agaaattatt atgaagtatt acattggtat tgatcttggc ggcactaaca tcaaagcagg
    12181 tgttgtaaat gaagaatttg agataatcgc aaaggctacc tgcaagacca atctgccaag
    12241 acctgcagag gagatctgcg aggatatggc taaggtcgct ttggaagctg taaagaacgc
    12301 agggctggag atagaacaga tagagtctgt aggtatcggc acacctggca ctgcaaattc
    12361 tgacactggc gttattgagt attcaaacaa ccttggtttc cttaacttcc acgttgttga
    12421 ccttatgaag aagtttatcg acaagccttg ctatgttgaa aatgatgcta acgctgcggc
    12481 atacggcgag tatgttgcag gtgcagcaaa gggtgcgaac gacgctgttt gcatcactct
    12541 cggcacaggc gttggcggag gaataatcat taacggaaag atatattcag gcttcaactt
    12601 tgcaggtgct gagataggtc acactgttat cgaccctaac ggtccgcagt gcacctgcgg
    12661 cagacacggc tgctttgagg tgttctcttc tgcaacaggt cttgtaagaa tgacaaaaga
    12721 ggctatgttc gaggacaagg actctatcat gtggaaaatg aacgaagagg atggcaaggt
    12781 atctgcaaga actgcgttca acgccatgag agcaggcgat aaggcaggca aggaagttgt
    12841 tgacaaatac ataaagtatc ttgcctgcgg aatcacaaac acaataaaca ttttccagcc
    12901 tgatattctc tgtatcggcg gcggcgtatg caacgagggc gaccctctgc tcctgccact
    12961 gaaagagctt gtggctaagg aaatttacac aaagaattca gcaaagaaca ctgagatagt
    13021 tattgcaaag ctcggaaatg acgcaggcat tataggtgcg gcattccttg gactttcaca
    13081 caagaaataa aaattattca ctgacaaaag aaaaggggta actacaatgg gattatttga
    13141 taagctttta aaggatgcgg ttgacgcaat agacaacgct gcggcaaaga acggcaatgg
    13201 tccgacagga cttaaagacc tgtttaatca ggctatggac aaaaaggacg aagctataaa
    13261 cggctcttca agctccacgg cttcaagcaa accaagcact gccacttcaa acacaagttc
    13321 aagcaggaga agcaattacg cttacacaag accgacatac tctgcaccgc aggaggtact
    13381 tgacacagca agcattcctg caaagccgtc aagagaaata gaatttgaga tatatgacgg
    13441 tgaagacgct gagataagag tgaaggtaac attcaacctg agcggagatt ttcttgaaac
    13501 ggccggagat gtacactatc tggcgtcata tcttccgacc attgatgacg attatgattc
    13561 agattactct gactcaaata ctccgctttt tggagtttat gatgcagatg aagaaataat
    13621 ctataacatg gcagagaaat ttgataatgg cggagtgcct gacgatgcaa tgatgttcct
    13681 gcctatccat gacatggatc caagagtgcg ttttaaggca aagatagaaa acagaggtgt
    13741 ggtgaaatat ttctattatt gccgacttta cgactatatc cctaactcca agacctgttt
    13801 ctctgtaagc taccctaaaa atgttgttgg tactccactt gagaaaaagc ttatggcggc
    13861 tgttgatgag actgtccgca caatgaagag agaagtatta gaagtggagc acagaccatt
    13921 ccgctaagga ggacaaaacg aaatgaagtt cagtataatt ggctttattc ctactctgct
    13981 tttgctgata cctgtgatat tcgcctgctt caagacacct gccgacaagc ctgagcttga
    14041 ggacgagaac attatcgttt tcatcgcaaa gtgcgtgggc agatttggct gcataggcta
    14101 tatgtgcctt gctattggag gcttttcaag caagctgctt ttcataatat gggctgttgt
    14161 tgcactgctc gttatcgcac tttattgggt gctttggata aggcagtata aaaaaggcaa
    14221 tcattttgaa gatatttacg gcactgggat cccgcttttc attatccctg cggcattcgt
    14281 gcttgtgaca ggtatcttct caatgaattt tatgcttatc atattctcag cgctggatat
    14341 cgtttgcgaa tactgctctg catacggtgt aagaaagcgt ctgcaccctg caaaggcaga
    14401 ggaaagctca gacgaggctg aaacaaaaga aaatgaggac ggagaataat accgctctcg
    14461 gtaatatatt tttcaaaaga ggtaaaaaaa aatggcgtgt aattattttt gtgacggagt
    14521 agacaaagct ccacagagat cacttttcaa tgctcttggc atgacaaagg aagaaatgga
    14581 cagacctctc gttggtatcg tatcttcata taatgagata gttcctggtc acatgaacat
    14641 tgacaagata gttgaagctg ttaaaatggg cgttgcaatg gcaggcggaa cacctgttgt
    14701 attccctgct atcgctgttt gcgacggtat cgctatgggt cacatgggaa tgaaatattc
    14761 tctcgttacc cgtgacctta tcgcagactc aacagagtgc atggctttgg ctcatcactt
    14821 tgacgctctt gttatgatac caaactgcga caagaacgtt cctggtctgc tcatggcagc
    14881 cgcaagagta aacgttccta ctattttcgt atcaggcggt ccaatgctgg ctggtcacgt
    14941 taagggcaaa aagacttccc tttcaagtat gttcgaggct gttggtactt acaacgcagg
    15001 caagttcact cttgatgacg ttgaggaatt tgagaacaaa gcctgtccga cttgcggaag
    15061 ctgttcaggt atgtacactg caaactctat gaactgcctt acagaagttc ttggcatggg
    15121 tcttagagga aacggcacta tccctgctgt atattctgaa agactcaagc ttgcaaaaca
    15181 cgcaggtatg caggtaatgg agctttacaa gaagaacatc agaccaagag atatcatgac
    15241 aaaggacgct ttcatcaacg ctatcacagc tgatatggcg cttggctgtt caacaaactc
    15301 aatgcttcac ctccctgcta tcgctcacga gtgcggagta gagcttgacc ttgactttgt
    15361 aaacgagata tcaaagagaa cgccaaacct ctgtcacctt gctcctgccg gtcacacata
    15421 catggaagac ctcaacgaag caggcggagt ttatgcagtt ctcagtgagc ttaacaagaa
    15481 aaaccttatc aacactgact gcatgacttg cacaggcaag acagtaggcg agaacatcag
    15541 cggcgttgta aacaagaacc ctgagcttat cagaccaata gacaatccat actcacagac
    15601 aggcggaatc gcagttctca gaggaaatct tgctcctgac acctgcgttg ttaagcgttc
    15661 agccgttgca cctgaaatgc ttgcacatga gggtcctgca agagtgtttg actgcgagga
    15721 ggacgctatc gctgctatca gaggcggcaa gatagttgag ggcgacgttg tcgttatccg
    15781 ttacgaggga ccaaagggcg gtcctggaat gagagagatg ctcaatccta catctgctat
    15841 cgcaggcatg ggtcttggct caactgttgc acttatcact gacggacgtt tcagcggtgc
    15901 ttcaagaggt gcttctatcg gtcacgtttc acctgaggca gctgtaggcg gtcctatcgc
    15961 acttgttgaa gaaggagata tgatagcaat agatattgac aactgctcta tcacacttaa
    16021 agtatctgac gaggagcttg ctgaaagaag aaagaactgg aagccaagac agccaaaggt
    16081 aacaacaggc tatcttgcaa gatacgcttc acttgtaact tctgcaaaca gaggtgctat
    16141 acttcagata aaggactaat atccgacaca aaatagtaag ggagaaaggc ggaataatat
    16201 gaaaaaattc atttcaggct tgcttggaat actctttgta gttcttgtag tagtaggtgt
    16261 tttcatctgt acatatgtat tcaatcagcc gtcaagatca ggcgattcaa gagagcttat
    16321 ctctggaaca tggacagata aggacgcaaa cattactttt gaattctctg acgagggcga
    16381 tttcactatc actcgcgctg atgacaaatc agttatcgca aagggctggt ttaagatcca
    16441 ggaggacgag ggaagcggaa agatacagct tcttgtaaat ccgtctgaca gagatacaac
    16501 agttgatatc ggtctgagaa tgaagttctt ctcgactatt tcctacaaaa acctttctgt
    16561 accgtctgag gacgagggcg acaaggtcgc tacctgcaag ttcatcttcc agaactctga
    16621 cggagtttac tcatgcgaga gaaaagactc cgaaggctcg ttctacgacg gcaaataacg
    16681 aattttcaag ggctacggct gaacgatatg tttagccgca gcttttgtgc agtaaagact
    16741 tgattaagat aaaaaaggag taaaaatgga gctactgatc aaaaatatcc gtgcctgcga
    16801 cccgcagaca gggcttgacg gtataacgga cataggcatt gacggaggaa agatagtcag
    16861 ccttggaaaa actgacgagc atgctcagag agttatcgac gcaaagggca agctttgtgc
    16921 attgcctggg cttttcgata tgcatgttca ctttcgtgac ccaggtctta cctacaagga
    16981 ggacatcatc acaggtgcga acgccgcaaa ggctggcggt ttcacgggcg tcgcttgtat
    17041 gccgaacaca aagccgccta tcgactcccc tgagggcgtg aaatacgttt ttgaaaaggc
    17101 aaagaaaaca ggtatcgacg ttttccctat tgcctgcgtt acaaagggca tgaaaggtca
    17161 ggagctttgc gactatgacg cactcaaagc cgcaggagtt accgccattt cagatgacgg
    17221 cagacctgtt gagaacgctg agcttatgcg tcaggctatc gaaaagacta acgagaacgg
    17281 tcttatcgtg acttctcact gcgaggactt gaagataata aacggcggca taataaacaa
    17341 aggcgagata tcagaaaagc ttggagtaaa gggcatggac agagcctctg aggactatat
    17401 cacagcccgt gagatatgcc ttgcaatgag ctgcaatgca agagttcaca tatgccacgt
    17461 ttccaccaaa ggctctgtga acatcataag agcggcgaaa aaggacggcg ttaaggtcac
    17521 ctgcgaaact gcgccgcact atttcacatt cacagacgaa aagcttttag accgtgacgc
    17581 agatttcaga atgtcgcctc cacttagaac tgaggaggac agggcggctg ttgaggaagc
    17641 tgttcttgac ggcactatcg actgcatcat caccgaccac gctccacact ccgcagagga
    17701 aaaggctgac tttgagaaag ccccaaacgg cgtcgttgga cttgaaactt cccttgcggc
    17761 aacgctcaca aaactttacc acagcggcaa gtgcggtctt gacaagatag cagagataat
    17821 gtcggtaaat ccgagaaaga taatgggact tgagcctatc aagatagcag tgggcgaaag
    17881 atgcgatctt tgtatctttg accctgacta tgaatgggaa gttatccctg cggagcttaa
    17941 ttcaaagagc aaaaacagcg tgttcaaagg cacaaggctc aaaggcaagg taatgtacac
    18001 aatatgcaga ggaaagctcg tttttgagct ttaatataaa aatatccgaa aggaagataa
    18061 aaatggcatt tgacagactt attgaaaata tcgtaaagac acagaacccg tcagtagtgg
    18121 gacttgaccc aaagcttgac tatgttcccg aatacatcaa ggaaaagaag ttcaagaaat
    18181 acggcaagac gctcaagggt gcggcaaagg ctatatggga gttcaacaag actataatcg
    18241 acgagataca cgatatctgc cctgctatca agccacaggc tgcatactat gaaatgtatg
    18301 gttacgaggg cgtaaagacc ctttacaaga ctatacagta tgcaaaagaa aaaggtatgt
    18361 tcgtcatgac agacggcaag agaaacgata tcggcgcaac tatggaagct tacgcaaccg
    18421 cacatcttgg tcttactgac gttggcggtg agcttgtgga agctttcggt gcagacgctc
    18481 tcactgtaaa cggctatctc ggttcagacg gtataaaccc gcttcttgag cagtgcagaa
    18541 aatatgacaa gggcattttc gttcttgtaa agacatcaaa caagtcaagc ggtgagcttc
    18601 aggatcttat gataggcaac aagacagttt atgccacaat gggctcaatg tgcgaactgt
    18661 ggggcaagga cgttatgggc aagtatggct acagcggtgt tggcgcagtt gtgggtgcga
    18721 cttatcctga gcagcttgct gaaatgagaa aggctctgcc gcacacattc ttccttgtac
    18781 cgggctacgg tgcacagggc ggcggtgctg agggcgtatc aaaggcattt gacgatcacg
    18841 gacttggcgc tatcgtaaac tcctcccgtg gcgtaatgtg tgcatacaag aaagaggata
    18901 actgtgacga gcatgatttt gcaaaggctg caagacgtga agttatcaga atgaaagagg
    18961 atataatctc atatctgcca aagatcacac ttgaataaac actaaaggag tgactgagta
    19021 tgacagcaaa gggcgttata ttgtttacag cgacggtggg agcattgctt ttatgctcct
    19081 gcgcaaagaa cgaggataca agctctttga cagaaagtca gcaggagcaa agcgatttcc
    19141 caagatgtat cgtaggtgag tggaaatgcc gtgaaacgcc ccttgacaac agcgagcttt
    19201 attcgggcta tctcagcctt attatccgtt cagacggcaa ctatctcatg tatgacgcag
    19261 aagcaggcaa tccctgctta aaaggcacta tcgaaatgga cagcgacgat cttgatctta
    19321 tctttgttga tgacagcgga gtggattttg caccccctgc agggtgggag aatatgtcca
    19381 aaaggcagga gataggctac actgctgact cttacacgct aaagcttact tatactgata
    19441 atgataactc agtcactctc gtttttgatg cggtgaacgc tccgaatgag gtaaagaact
    19501 aagtacaggg aggattttta atgtacaagc agggtaaata cccaatagtt tcaaaaaaga
    19561 cccttgcaaa aggaattttt gacattgaga tatactgtcc gcacattgca aaggcggcaa
    19621 aggcaggaca gtttgcacag gtgcaggcag agggcttttt cctccgcaga cctatctcta
    19681 tttgcgatat caacaaggac aagggtacta tccgccttgt ttttgaggta agaggtcacg
    19741 gcactgacaa gataagcgag cttaacaagg gcgaccttgt tgacattatc gcaccgctgg
    19801 gtaacggctt caaggtgctt gaaaagggca aaaaagctgt ctgtatcggc ggcggcatcg
    19861 gcacgcctcc aatggtgggc attgcaaagg aatacggcga aaacgccact gttataagcg
    19921 gtttcagaaa cgctgcggcg gtcattttgc aggaggactt caaggccatg ggctgtgacg
    19981 tcaagctttg cactgacgac ggctcggcag gaagaaaggg ctttgttact gacgcacttc
    20041 ttgacgtgct taaaagcgaa aagcctgata tcgtttatgc ctgcggtcca atgataatgc
    20101 tcaaaaatat cacgaagata tgtgaggaaa acggcattta ctgtcaggta tctcttgagc
    20161 agagaatggc ttgcggcgtg ggtgcgtgtc ttgtatgcgt atgccgcact gtcaaggacg
    20221 gtcaggagtt caattctcac gtttgcaagg acggtcctgt attcgacagc gaggaggtag
    20281 tgttcgatga ataggataaa tgtgaatttc tgcggagttg agttcaaaaa ccctgttatc
    20341 cctgcaagcg gaacatttgg ctacggcaga gaatacgagc agttctatcc cctttcaaag
    20401 ctgggcggaa tatccgtaaa aggcactacg cttcaccgcc gtgagggcaa cccaggtccg
    20461 agagttgcgg aaactccaag cggtatgctc aattctgttg gacttcaaaa cggcggcgtt
    20521 gacaagttca taaactacga gcttccaaac cttgtaacaa aggacacaag gataatcgca
    20581 aacatagcag gctcaactat cgaagaatgt gcagagcttg cggaaaagct caacggcact
    20641 gctgttgaca tgatagagct taacatatcc tgccctaacg taaaacaggg cggagcggct
    20701 ttcggcactg actgcaacgt tgcaggtgcg gtcacaaagg ctgtaaagga cgttacagaa
    20761 aagcctctta tggtaaagct ttcaccgaac gttacaagca ttgtggacat tgcaaaatct
    20821 gttgaagcaa acggtgcgga cgctgtttcc cttataaaca cgcttctcgg tatgcgtatc
    20881 gacataaaga cacgccgtcc tatactgaaa aacaacgtgg gcgggctttc tggtcctgcg
    20941 gtgttccctg ttgccgtaag aatggtatgg caggtggcaa acgctgttaa aatacctgtt
    21001 atgggcatgg gcggtatcgc cacatgggaa gacgccattg aaatgatgat ggcaggcgca
    21061 ggtgctgttc aggtaggtgc ggcaatattc gctgaccctt atgcacctgt gaagatagcc
    21121 gagggtatgc agaaattctg cgaagacaac ggtataaaca acatctctga gatagtcggc
    21181 actgttaagc cttggtaata acatagtaaa atatccgtct ttttacaggg cggatatttt
    21241 tatggtattt tttcttatga atttcttaaa ttcattgaaa acagcttgca a
//
LOCUS       DAWEMR010000020        20345 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1102_length_20345_cov_10.484278,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000020 DAWEMR010000000
VERSION     DAWEMR010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 20345)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20345)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20345
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1102_length_20345_cov_10.484278"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6177..6186
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6982..6991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10771..10780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5848 a         4526 c         4224 g         5717 t
ORIGIN      
        1 tcagttcttc aagggtacct atttcattaa acaattcttt tttcatgtat attgtaatat
       61 actgctggtt agtggtgaat ttataaggct tatagaaata tactctctta acagccacat
      121 aatcatcaac atctatatta ggatatgcct tggttatttc acagcggttg atcacaatgt
      181 caggctctcc ctgttcctgc cactttgtaa aggtttcttc agcttgttcc tgagtatttg
      241 cacgataata caaaaatact ttgttaggat cgtctatatt aaaattatag aaaatattct
      301 tgtcaaaata tgagtccaac gatacagata caccctgtat ttcaggataa tcataccatt
      361 cataatcaga ttcatcagag ccgaaatagg tataatcctc agggtcgccc ttgataagct
      421 gctgccacat tcccattatt ttataatcat caaggtgata ttcttttata aaatcagcag
      481 cttcatcaaa ccaatatgga taacgaatgt caaagtaaga agaagctcca ctccatgcta
      541 tcggtatcaa aacgggcaga aatactattg cctttatgac aaacttaagc tttccaccta
      601 gtttatcata aagtttcaac atacgatcag gaagctgaac actgccgttt tcttcaacga
      661 ttatccaaaa tgtgaatatc acaaaagccg tcactacgcc tatatgatgt ggcgacgcat
      721 agacgatcga gccaaacgca ccaactatgc tgtatggtat tataaaggtc aaaagcttcc
      781 tgtttttatg caatactacc atgaaaatta taaatacaaa tatcaatact attatagtag
      841 ggagttgttc cgcaaggtcg ctgatatcca tgccataatt ataaaaagac gtaaagatcg
      901 catctgatag cataacaaaa taaaacaata tgtttccaac gccaattgga aatgacattt
      961 ccgtttcact tgtagtcttg ccaccgagaa agacgttttc atctggcaca atagccgcca
     1021 tgaccaacaa cgcaaatacc agcagacaaa acatcagcca acagcggcgg tctttcagta
     1081 tgcctgcaag ttttccgctt tttttatatt caataaaaat ctctatgagc catacgatac
     1141 acagaccacc tgcgatcatt atgccatatg aatgcacagc gcacatgagc gcaaggcaca
     1201 aaaggtattt tacagggtgt tcatttctat tcttatagca cactgccgca agaaaaatcg
     1261 caagcacaaa tatgcaatag ggtctgctta taacgccgta ctgataaaac aggaagaagt
     1321 taaacggcaa cagacaacgt atcagctttg gaaaaggtga cttgaaaagc aagaccgcca
     1381 cagcaagagt catgaaaaag atatttacta tggcgaggga cagctcgtat ggtgcgccaa
     1441 gcttggcaaa tggaagaagt atcagatccc atagaggagg gtgaccttca tagtgtggga
     1501 tctcaaaaag tatctctttt aatgatcccg aacgtgcaat cgcccacgcc tgagcttcat
     1561 caaaccacgg ttcatgaaaa aacgatacag tgcaaacacc aagcaggtat attatcagcg
     1621 ttataacatc gcattttcca tgctctttgc tgaacgccca gcttttaaat ttctctttca
     1681 ttaatatcct ccaaaaaaat aattgacata taaattatac catttattaa gaaattagtc
     1741 aatgtttttg taaaactttt tgcggcaaat attgacatga ttaggcttgt gctcaatgtc
     1801 tgcaataaaa agaaataacc gtcccacgtc caataggtcg gttatttctt aaagaaaaaa
     1861 ctatattgga ggtaaaccgc ttacagcagt atgcctcttt tttgttatca taattattcc
     1921 acggcaatga cagatcgtca atagtttcag aatttaacgc cccaactgaa attctcaggg
     1981 agatcgtcag gtttgttcac agggtcagga tactgcccta caatgccttt agaccttggc
     2041 gaacttgtat cacaccactg tgcaagcttg tatctgccgt cctcccctgc tatccaaaag
     2101 gcatagcctg cattgtcgcc attgacgtta agtgcgtcat ggacggcttt ttcaagcttt
     2161 ttggattcgt caggctctgc ggtgcaatcg accacagccg tacatgactg aacgtcgata
     2221 tcgttgccct cacactgcat atcaaacgta acagcgttca cagtattaaa aacaatcttg
     2281 gcattgctgt ttgcaccctt aagcttcgct tttctgatat aactcatcat agttggcaca
     2341 agaatagccg caagcgcgcc tattatggca ataaccacca caagctccac caaagtaaag
     2401 ccttttttat tcatgtcttt ccctcaattc tttcatcaaa atatcgatcc ccattaatat
     2461 ttcgacaagt atattataca aaatttaccc gattttgtca agaaaaaggc aagctttatt
     2521 actcaatttt accatttgca caaaggcaag ctatttttat tggctatttt atatattttc
     2581 agacaaaaaa agaaccgctc aaatgagcgg ttctgaattt ttcttttaat tagaaagatg
     2641 taccgatctt gtgaagtgta tcgtaatcgg atgttgggtt tggccactga ccaacaattg
     2701 tatcagttga tgaatcagct gaagaccact gagcaagctt aacgttcttt gtagtgccgt
     2761 caatcgtcca gtaaacataa ccagcagcat caccattatc tgagagagcc ttctttacag
     2821 ccttctcaag aacctttgaa tcatcaagag aacttactgc tacaggtgaa gcagtagctg
     2881 aaatgctacc ttccttgccg tcgacgataa ggtcagcagc ctcgttgttt acagttgtga
     2941 aaacgagctt agcgtttgag ttagctgtct tgagctttga cttctttaca tagttcatca
     3001 ttgtaggaac gaggatagct gcaagaatac cgatgatagc gataacaact acgagctcta
     3061 caagggtgaa accttttctg ttagatttct tcatagaaaa tccctccata ataaattaaa
     3121 aaaatttgtt tataaaagag ctgcaagccg atttaagcag ctactttcca ataattaagc
     3181 ggctttcgga gttctttgtg ttctccttat caccatgatt aaagtataca tcatcttgtc
     3241 caaaatgtca atagtttttc agattttttc tcaagtttgt tcaacttgac gaaaaactaa
     3301 ttttcaatcg gtcattttga cgattggtaa cactatgttg acattttaac aagaaattat
     3361 gtcacacacc gttttatgat gtatcacctt acatcgttct taattatatc ttaaaacaaa
     3421 taaattgtca agacttattt aacagtttcg ttatttttgt gtactctgta cacttcccat
     3481 tcatttttga atacaatttg cactatttgt ttcgcatatt taactatttg aatgatttct
     3541 tcatcttatt atacagattt tcagaagcgt tttctatctc cgccctgcca taataaagcg
     3601 actcggaggt gcttataaaa aggtcaagta ccccctgatc ttcaagcttc ggggacatta
     3661 tttcaagctc ctctgagttt gccattttct cgttggcttt atactctatc ccgtcaagca
     3721 tatccctcgc ttcaagggct tccaatgccg cctttgatgc aggaacgccc tttcccatgc
     3781 cctgcaggag agtcatttcc tcaccgttca caagatagtc aagaagcttt gcggcttcct
     3841 caggggactt ggtatttttc tttatggcat aaacgctagt gggcttttta taccaaccgc
     3901 tgctcacagc ctgcttatgc tttggatagt cgcctataac gatgtcaaag ccaaacttct
     3961 gagcaggctc gcagtaatac tctgcgtctg atatccagga agccacacct gctgtcaggc
     4021 acttttcaag atcgttccta tcgaaatccc acgcaggttt tgtgaccttt ttattcagca
     4081 tttcctgata aaaggctagc atgagcctta cgtcctccga ggtatactga aactcaccgc
     4141 tgtcagataa catttttctt cccgttacct gctccacata cgccacagag ctgagataag
     4201 ccgccttttt cgtcatttca acaggataca cctcgtctga tttcatgacc tctgccgcat
     4261 ttataaggtc agaccaattt tcaggcaggc tcagaccata gctgtcatac atcgttttat
     4321 tatagtagaa ggttatacag ttcgtaccgg taggcaaggc attaagtttg ccgtttattg
     4381 tgccatatga aaggctgtca tcatcaaatg ctgagagatt tatgtattcc gacagctcat
     4441 tcaggtcata aaattcctcg ccgtcgggag aatattcata gagccaactg tagtttaact
     4501 gcatgatgtc tgcggtggtg tcagagaaaa attccacgtc catgcgtgtt ttgaagccgt
     4561 caaagtctgc atactcaggt cttaccacta cgtccttatt gctgttctga taggctttaa
     4621 gcccgtcgag agtatactcg ttacgcgcgt ccttgcccca ccacgagaag gacacctcgg
     4681 tctttacatt atgctccatt cttctttcaa acaatgcaca gcccgtcatg acagtcactg
     4741 tgcagatcgc tgaagcggtc aaagccgcac accttacaat gcgtgttctc aactgcggct
     4801 cacctcttcc tcttttttat catataccac cgctcttgcg tcctcactgt gttctgcttt
     4861 ctcgcaggct ttttctgcca tttcgtaaag ttcatcataa tcatcgcttg tttcagggta
     4921 gaccgacgca cccactgcac attttatgta gcccctgtca tcgtcaaggc aacattcctt
     4981 cagcttatcg ttaagctctt taacataagc cctcacatta ccataggctc tcacaaggtc
     5041 aaagttcgtg tggtctgcaa acagagcaaa ctcgccctcc ctgaaaattc cgcagatatc
     5101 gccctccttg cggttctcca ccagtatttc ggcaaccttt cgctcagctt ccgccactat
     5161 ctcctcgcca tagttgagcc ttataagctc cagatttgtt attttcacga taataaatgc
     5221 gatagtcgag cctgagatac aagtttccat tttatccata atgctgtttt cggtatattc
     5281 cgcagtgaaa agccctgtat tttcgtccac ctttggatat tttccaaact tcggtccgtt
     5341 ctgcgggtcg ttatccgccg ctgctgccgc agatatcata ataaatatga ctgcgcagca
     5401 aataaagata ccaaggcatt taagccctgt tttcacatag tgacgcaaag tgtctgacat
     5461 atccacggta gtaatgacca cccaatcgtc actgcaatca tcaatagcac agatagtagt
     5521 catatccgaa acggtgacgc cagtgcctcc atcaaggaat ttagagagct tttcatcgag
     5581 cttttcgccg acaacacttt ttccgtcgtt agcatagatt atcctgccat cgtcatcaca
     5641 cagcatggta tttatctcgc tgtaattgtc agagggcaga aaaacaagat caaactccgt
     5701 tgaaaacaca gaggagataa aaataagatc gtcatttatc ctgcctgcga aatacactcg
     5761 tctgtagtta tcgtcctgtc ccgtgaacca ttttatcttg ctgtcaccca taaggtcgga
     5821 aactctcttg tatatctcat cgtcaaagat atcctttgta ccatcggtta tctttcctac
     5881 attatgttca ttgctgctta caatgccaaa atctgtatag ttgcccaatg tactaagctc
     5941 tataagcctc tcagtgagaa cattctcctc attaagcaca gcaaattcgc tctttgacgt
     6001 atcagtagga tcatatttcg catagtcttc attgcccatt ataagcttgg cagtgtcctc
     6061 cattttcttc ataaaggcag ccacattctg gtctgcttcc gcatttatgg cagagcaaag
     6121 ctgagcctct ttctgaaatg cgttcacacc gctgtaatag caggcacacg catacannnn
     6181 nnnnnncaca agcagacaag caacgaacag cactaacacg cttgttttca aaatagatct
     6241 tctatatttt tttattttat catacagttc aaaatcagca gaatttttca aaagctatat
     6301 cacctctcag gctaaattac tataatcatt attaattata acacatatgg ccataaaata
     6361 agtgattttt ttgttaatta ttgctcaatt tgtattgtat ggtttatatt ttctctgaca
     6421 cggcactgtc tgaccttatt tgcatggcgt ttctttttgg tttccgcttc aaaaagcgac
     6481 agtgccgccc atgccgctgt gaacacgatg cctgcggcaa acactgctcc catgttcctc
     6541 tctgccaccg caacaaacag gaaaaacagc accattctca ttatagtttt cataagccac
     6601 ctgtcgccgc ctgcacctgc cgcaacgata aaaacattca tcatgattat ccatatcatt
     6661 tttacacaca tcctcatatt tttctttttc tatttcgggg ctgtatgtat gataacacaa
     6721 cttgatgaat ttgaggggta attttttgga cagcaagttt tttgtcgata tgcacagata
     6781 agaacgtttg ttttgatata aattctacaa atccaaaaga ccccatggca aaagccacag
     6841 ggtctttcag agaagtaaga agtataatat gaagaaaaaa ttatgaatgg ggaatgctaa
     6901 agttatgtat ttttccatgc catttcactt ttgccgcact ctgaaaaatc agagcacagc
     6961 gagatgaaat gaaaagaaga annnnnnnnn naagaagaat gaaggaaaat tatatatgaa
     7021 aactcattat atgaaaaact cattatgagt aattggttga cggaactttc atcaagctct
     7081 caaagctttt gcctgaggtc tttctcggtg ttcctctgac cttgtatata taatacctgt
     7141 tcaatatgat agttttatga aaagacactg aaaataagct gataagaaaa gcaaagtggt
     7201 tttcataata acgtaggggc gaacagcgtc cgcacacaaa acgcaagtca atccgccgcc
     7261 tgcatattct ctatcctgat gtgactacaa gcacatctcc ctctttgagc cgcaacctgc
     7321 cgtttgggat aagtatacgc ccctgacgtt ttatcattac tatcaggctg tcctctgaca
     7381 ttgttatttc cgacactttc agaccgcacc actcgtgatg cttatcaagg ttcatttcgc
     7441 ttaggacaaa atggctgtca tcataaaagc ctgccgcact cagcaccaca acatcccctg
     7501 caagtatctg ggtttttcct gttggtatta tcttctcttt gtcacggagt atcatcacca
     7561 caagcagacc tctcggaaaa ggtatatcct ttatcttccg ccctgcccaa ttgctgtccg
     7621 gctgaacgga aagctttata aactgcactt ccgtttcgtc gctgtagtca ttgaacgttt
     7681 tcattacgtt ctcctcatcg tctatcatat cgagcttttt agcaaacagc ggcagaagcg
     7741 taccctgaat acctatagac ataagcacga tacagaaaac gatatggaaa acgtcatttt
     7801 tcatatatgc gtcatcaact gtcgccatga tagcgaaaac aatggaggtc gctcctctta
     7861 gtcccgccca tgagataagg gctatctgct tccactttgc accgaaaggc ttcatgataa
     7921 cagtaacagc cgcaggtctt gcaacgaatg taaggaacaa tgctatcgcc gccgcaggaa
     7981 gtattattga cggcatttgt gacgggaatg ccaaaagtcc aaggaggaaa aacagcaata
     8041 tctgcgtgaa gcccgttatt ccgtcaaaga aatgcacaag ggctgttttg cctgatattt
     8101 ttgtgttgcc caggactatt cccacaatgt aacagctaag atagccgttt ccgcccaccg
     8161 ccgagggcag agcataggaa agaatagcta tggcagtaac aaagatagcg tcaaagcctg
     8221 ctgcgtcaaa tcgcacacgt tttatgagat acactgcgcc caaagatata agcgcaccga
     8281 agcccacacc gaagaacacc tgtgcaagca agctcacaac aaagcctcct gccgtcgcac
     8341 cgcctttcat tactgaaagt attataaccg tgagcatata agcccaaggg tcgttgcttc
     8401 cgctttcaag ctccagtatt gaagccgtac cgtatcgcag gttcagcttt ttgcttctaa
     8461 gtacagagaa caccgaagcg gcgtctgtgg agcttataac agcgcctata agcagacttt
     8521 ccagccactc aaagcccagc acaagcttgc agaatgctcc agttgcaaag gctgtgagca
     8581 aaactcctgc ggaagcgagt ataagtgact tgcctgccac ctttttggct tcgctccatt
     8641 tcgtgccaaa gcctccgtag aagattatga aaacaagtcc caccgagcat atctcctcgg
     8701 caaatttata gttatcgaac tctattttaa aaagcccgtc agagccgaac aacattccaa
     8761 ggagaatgaa cgccagcaat gcaggaagtc caaacttgtc cgatatttta ttgaaaaaca
     8821 cacagcacag cactatcaca gaagataaaa tgagcattgt ggtcaagtat atcactcctt
     8881 tcacaacaaa cacatcgcat ttactgcgat ctcatttgtg tatccgcaca atgttgatct
     8941 acacaaattc cggataattc aagcctcgtc acttcgatcg ggacgtcgag ggcgccgtcc
     9001 cctacaattc agaatttatg gcaatttctg aacacgggcg aacactgttc gcccctacat
     9061 atagatatgg cagttattgt cttattataa aaatagcgct ttgggtatct tctactcaaa
     9121 gcgcctttgc ttgtgtaaat attataacat attcacccct gtgctgtcaa ctttttgccc
     9181 acatcttacg gaaattttac attacccctt gacaatatcg aacatatgtg ctacaatcaa
     9241 ttgtgaagca gtatatccaa ataaaaaacg cctgcctttt acagcagacg ttcgcctatt
     9301 tatatctcgg ctcttttatc cttgtccaaa tttttatttg tagcaatcgg caatatctga
     9361 aatagcgtcc tttgccattt ccactgccct ttcaggtcca agcacctttg cctgtgagcc
     9421 gaactggaaa agccagcccc agaatgttgg tgcgacctgt acctcctcat ttatatagaa
     9481 ggtattgtcg gaatttttgt gacatatggt tttctcacca aaacgatcca ttacaacatc
     9541 tatgagctta ttgtcaaact gcaattgcag cgtcataagc tcgccgccgt acattccgta
     9601 gatagtctga cgatttgtga cctctgtctt ctcctcctcg gaaagctccc tcctggggat
     9661 atctgtaaca gtgacctgag tcattctgtc aactctgtag cggcatatct tttcatgctt
     9721 ttcgcaatag cacacaaggt aatagttgtc gttttcccaa accagagaat acggtgagac
     9781 cgtatacacc tcgccgccgt ggcgaagctg tttgcgtttt tcaacggtgt attcgtaata
     9841 cttgaagcgt atctccaagc cgctgaaaat gccctcttgt atgctgttta tagcatagta
     9901 tatctgctca ttgaaggtct ttgtcctgtt tgacacatag accattcggc gcagatcctt
     9961 tgccttgtgt tcagatgtaa gtgattgtat cttctttaac agctccttcg atttcttctg
    10021 tgtaagaaac ctgcttgaag caactgcgtc tgcaagaaca tacagctcct cgtcctgaaa
    10081 cagcctttga gcaagataat aagcgttgga gtgaccgtcc ttagttatct caatatccat
    10141 tccgctgtcg cagagggtct tgatatcgtc atagatagtc ttacgctcag cttttatgcc
    10201 gtagtcgccc aggatctcga taagcctgtt tcctgtaatg gtgtgctttt cgtctgtctt
    10261 ggtctcaaaa agctttttca tggtcaaaag cttctgtttc tgtctggaaa ctcctgtcac
    10321 tttattttat cctccccgct gcgtttcata tccgaaacct taattcttga gccgtccggc
    10381 tcaatgatcg tcgtggcttc aagctgacct gtcaggttag ctataacgct tctgcgatac
    10441 tcatttctca aagcctcacg ctcagacttc tcctctgcgg taagctctct ttttctcgca
    10501 atagcagtaa gctcagacaa tctgttaagc tttactttct ccataaattt ttacacttct
    10561 ccttaaagct tcacactgtc ctgtgttttt atttttatcc gcagtattcg gctcaaaata
    10621 gaacgggcag cttacacaat accgcccttt tcattcaaaa gatgttcata tcggcttttt
    10681 tctttattta tatttataag tccaacaaaa caccctcaaa caaatttgca gatacattat
    10741 aacacatatt tctttaaatt gcaatggtaa nnnnnnnnnn taatttccca caaattaaac
    10801 aagaattttg aaaattgtgt ttgtccaaaa atttgcccac aatatatagt gggtaaaaaa
    10861 atggtcagaa aacatcgaaa aaagataaag aaaggatttt tatgcagcac acaaaggtat
    10921 catacgagct tatacaaaac tatatctcaa gttctcgtga cactgagaaa gatagtcccg
    10981 accctgcctc tgccatagaa aacaggctca tgcgtgagat agtgcgggaa aatttgacga
    11041 aacggcagaa atgttatata ataatgtatt atacaaaggg catgacaatg caggagatag
    11101 ccgacgcaga gggtgtttca aaatccacag tatccaggac tatagggctt gcaagaaagc
    11161 ggatatcctg cggcatgaag cctgccatgc tcaaaagcgc actgaaaggg agatagcatt
    11221 gaacgcacct gtgaataaat tcgttagaga atatgcaaag tcaagcatag cgaggtttca
    11281 tatgcccggt cacaaagggc agaaatttca tggtcttgag gctcttgaca tcactgagat
    11341 aaggggtgcg gactatcttt ttgaagccga ggggatcatc gcacagagcg aagctaaaat
    11401 ggcaaaagcc tatggcagca ggcggacttt ttactctgcc gaggggtcga gcctttgcat
    11461 aaaggcaatg cttggcatag tatgtcgcag tggacaagga aaaaagataa ctgttgccgc
    11521 accaagaaac gtccacaagg cgtttataaa tgcctgcata ctccttgaca ttgacgtgga
    11581 gtggatatat ccaaagggtg agtttacgtc tatatgcgca ggaagcgtca ctcccgagga
    11641 cgttgaggag gtgctgtcaa ggacaaaggc agattgcgtt tatgtgacct cacctgacta
    11701 tcttggcgat ctcagcgaca taaagggcat gagcgaggtt tgcaggaaat acggtgtgcc
    11761 tcttctttgc gacaacgctc acggagctta tctgaagttt ctcactgaga gcctgcaccc
    11821 catggacttg ggggctgata tgtgctgtga ctctgctcac aaaacgcttc catgctacac
    11881 agggggagca atgcttcata tttcaaatca cgcccctgtg aattatgatg attgcgccaa
    11941 agagataatg tcaatgttcg gctcaacaag cccctcctat cttataatgg agagccttga
    12001 cctttgcgct gactatgtga atggtgattt tcctcgtgac cttgaacgcg ctgtggaaag
    12061 ggtgaaagtc tgcaaagata ggataacagc tttcggctgg cataccatag gcgaagagcc
    12121 tatgaagata accgttgccg catacaaaag cggtatcaca ggtgacgacc ttgcagacag
    12181 gctgagggaa tatggcatcg agcctgaata cagcgacaca caatatgttg tgcttatgcc
    12241 gtcgcctttc aacagcgagg aggatttttt gaaacttgaa aaggcgtttt cagagataaa
    12301 acagcctagg ataaggcttt tcggtgactt acccgctata ccaaaaggga taaaacgcat
    12361 gggtataagg caggcggctt tttcggctca gcacgccgta aacgtagagg acgcctgcgg
    12421 tcttatatgc ggaatgaccg tcacaagctg tcagccctct gtgcctgtgg cagtgagcgg
    12481 tgaggagata acaccgcagg taataaaaat tttgaaaaac tatggaattg aacgcataaa
    12541 tgtgctataa tataaggtgt agctaaaaaa gctattatta catcaaaata acaggaggca
    12601 aaatcatgaa acttgtctat gctatcgtaa acagcgatga tacctacgct gtaaataaag
    12661 gacttctgaa agccggcata agagccacaa agctttcttc aacaggcggt tttcttatgt
    12721 caggaaacac cacctttctt atctgctgcc ctgatgagca ggtagacctt gttatagagg
    12781 tctgcaaaaa gtattcaaag aaaagaaagc agttcgttcc gtcatcaact atcatggagc
    12841 ctgacggtgc ggcttcatat cctgttgagg tggctattgg cggcgcaact attttcgtta
    12901 ctgatatcga gcgttttgag aaagtctaag ggatagaaaa ttttcagaaa ggggtctgag
    12961 ggcttgataa tatacggaaa cagcgagcct cgcaggctcg tatctgagat gataaaaaag
    13021 aaacgagagc cgcactccat aatgatacac ggagaaaagg ggcttggtaa aaagaccctt
    13081 gcacggtgga tagcggcggc actcttgtgc gaacgtcaga caggcgaacc ttgcggcatt
    13141 tgcaggacgt gcagaatgat agccgacgga gctcaccctg acgttatggt ggcaaaggca
    13201 aacagcaacg gcaactatat cgttgacgat tctatcagac ctatagttgc ggaagctccc
    13261 gttgcaccga acgaggcgag aatgaaagta tacataattc ctgaccttga cttgtcggtg
    13321 aacactgtta ttcaggtgca gaacattctg cttaaagtca ttgaagagcc gcctgagcat
    13381 accgctataa tacttacggc aaggagcaag gaaatttttc tgcctaccat aatctcacga
    13441 gtgctttccc ttggcatgac ctctgtcacg gaggcggaga gcatgaagtg tttgcaggag
    13501 aaatatcctg ccgttgcccc tgcgctcata agcgaagctg tttcagcagg gcgtggaaat
    13561 atcggcagat gtgtcgagta tcttgaacac tcccagtttt ttgacagcgt gaagcttgca
    13621 agaggtctgt gcgacgcaat gtgcggtgga aacgaatatg acgttttgaa aacgctattt
    13681 gcggcagacg gcaaaaaaac gctgctgaga gaggggctga gcctttttca ggagatactc
    13741 cgtgacagtg cggcattcag gctcggggga gaaagtgaaa agtctgtgag ctgtgcgaaa
    13801 gagggtgcaa agacgctcag cagaagcctt tcctcagatc aggcggtcag gctttacgat
    13861 atagtttctg actacatagg caggatagac gcaaactgca acataagcct gactataaac
    13921 agtctggcag gacagatata ttctgtcaca ggaaagtagc tgacggctta cggctgtgtt
    13981 acaaaaaatt gcagggtgaa cagttcaccc gtttggatac agatctggca cagcggtcat
    14041 gccgtgatat atatttgaaa ggaaaaagag atgttaaaaa taatcggagt acgctttaaa
    14101 agcgttggca agatatacta cttcgaccca aaagactata acgtgaaaat gggcgacaaa
    14161 gttatcgttg agaccgcaag gggcgttgaa tgtggtgaag ttgcgcttgt ggacagggaa
    14221 atagacgaaa caagattcac aaaccccgta aaaggcatta taaggcttgc cacccctaac
    14281 gatatgaaaa ctatcgaaaa aaacagacaa aaggaaaagg acgctttcaa gatatgcgag
    14341 cagaagatag ccgctcacaa gctgaaaatg aatcttatcg acgttgaatg tacttttgac
    14401 aacaacaagc ttcttttcta ttttaccgcc gagagcagag ttgactttcg tgagcttgta
    14461 aaagaccttg ccgcggtatt cagaacgagg atagagcttc gtcagatagg cgttcgtgac
    14521 gaggcaaaaa tgcttggggg tcttggcatc tgcggacagc ctttctgctg ttcaagattt
    14581 ttgggagagt tccagcctgt ttctattaaa atggctaagg agcagggctt gtcacttaac
    14641 cctacaaaga tctcaggcac ttgcggaaga cttatgtgct gtttgaaata cgagcaggac
    14701 agctatgagg atcttctcaa gcacacacca aaggtgggcg ctatcgtaaa aacggctgag
    14761 ggcagaggcg ttgttgagga agtaaatctg ctcacaggca agctgagagt taaaatggat
    14821 aagaacgaca atgttgttgt ggtaaagaaa gacgatattg aggtaataaa ggattctgtt
    14881 ataaggctcg accgtgacga gataaaggct ctcaaggggc ttgagggcaa gtaatttcag
    14941 cgctttgcac gagcgatcac cgcaacataa acgggctgtc gggggcgcca gcccctacac
    15001 aaaatcggcg cagcattttt atatatcagc aaatcatcag ataaatatgc agaaaggaac
    15061 gtccatatgg caaaggtgaa aacttcgtat gtgtgcagtc aatgcggata tgaaacttca
    15121 aaatggaacg gcaaatgccc taactgcggc gaatggaaca cctttgagga ggtcgagctt
    15181 gcggtcagag cgggcggtgc aaagtctaag cccacgactg tcagggatat ttccgacaag
    15241 atagttggca taggcggtgt tgacgcaagc tacaacgaga tacgttatgc aacggggctg
    15301 aaagagcttg acagagtgct tggaggaggg cttgtgaaag gctctctggt gcttctgggc
    15361 ggtgaaccgg gcataggaaa atccacaatg cttttgcaga tatgtcagta tttaggacaa
    15421 gaccacacca tactttatgt atcgggagag gagtctgtac ggcagataaa gcttcgtgca
    15481 aacaggcttc atgttgattc tgaaaatctg tatcttcttg cagacaatga ctgcgagcag
    15541 atatgctctg tgatagtaaa agaaaagcct gagatagtca taatcgactc tatccagacc
    15601 atgaacataa gcgggatatc ctcagcgcag gggtctgtga cgcaggtaag agaatgtaca
    15661 aatatgttca tgcggacggc aaagtcagaa gagataccaa tgttcatagt aggtcacgtc
    15721 aacaaggacg gtgctatcgc aggacctaag gtcatggaac acattgttga ctgcgtgctg
    15781 tattttgagg gtcagcgaaa tctcacctac aggatactgc gagccataaa aaaccgtttt
    15841 ggctcaacga atgagatagg tatgtttgaa atggctgaca gcggtctgct ggaagttgaa
    15901 aatccctcta tgatgttttt ggagggcaga ccgacagacg cttcgggaac ttgtgttgcc
    15961 tgcataatgg agggcacaag gcctgttatg gcggaagttc aggcacttgt gtgcaagagc
    16021 gtgcttgcct ccccaagacg tactgccaca ggctttgact actacagaat ggcgataata
    16081 atagctgtgc ttgaaaagcg gcttggctat tttttcggtg ggctggacgt ttacatcaac
    16141 attgtaggcg gattaaagct tgatgacact gcggctgacc tatcggtggc gttggccctt
    16201 tattcagggc tgacggacaa ggttataagc gacaagctta tcgcccttgg cgagataggc
    16261 ttaggcggcg agcttaggag catttcccac tgtgaacagc gccttgcgga gtgcgaacga
    16321 atgggctttg aaacttgtat cgttcctgca cattcgctga aaagcgttga cacaagcaag
    16381 ttttcaatga aaataatcgg tgtgcgctca ataagagagg cgttcaaagc tataggctga
    16441 taagctaata cagaacaaca caaaacggac ggaagaccaa atcttccgcc cgttattttt
    16501 ctgtcttatg tagaagccgc acgctctccc ataatatcgc ttctgagaag cccatagata
    16561 ttgaaatctg tatagatgtt cacaaacttt gcctgacgct ttgttccctc acaggtaaat
    16621 ccgcattttt ccattactct gtttgaagcc acgttcaccg tcatgacctc agcttcaagc
    16681 ctttgaacgc ctgtttcctc aaagcaaaat ctgatcacac gcctgagagc ctcggtgaca
    16741 aagcccatgt tctgatacgc cgccgagata cgatagccta tcattgcctg tctgtcattc
    16801 tcgatattga agagatttac ctcccctgcc aacctatgat ttttctttag ctctatcccc
    16861 cagcttatat gatccttttt gccgttgtta agagtgcgtc cgaaaaaaag cgacggctct
    16921 ctttcctgcc ttgtcatatt tctgccccaa tatttgtaaa ggttctcgtc agccatatag
    16981 gttttcatat cctctgcgtc agtagcggtc ttcgccctga gtatgagtct ttctgtttcc
    17041 agcacaggaa aatgagaaaa ataatagtcc atatcctgca aaataaacac tctcccctgc
    17101 ttatataata aaaattccca agtctactaa aattataaca taaaatccag caaaaatcaa
    17161 gcgtttttaa gcgaaataca caaaaaagca gaaaacgccc cgaaacaagt tcgggacgct
    17221 ctctgttcta taatctggag aattagaaat caacttctgt atcgtagtag cagcacttga
    17281 ggagcttttc catttcttcc tgtgaaggct gacgtgggtt agaacctgtg caagcgtctg
    17341 cgatagcgtt cttagcgatc tcagggagtc tttcaaggaa tacgttctct ggaacgaatc
    17401 cctgctcaca tggatagctg tctgtaccat agttcttgat gcagtgagga atgttgagct
    17461 tatcattcat gcctcttaca tactcgatga ggagctttac cttctcgtca tcgtttgaac
    17521 cacccaggtg catataatca gcgataacgc catatctctt cttagctgtt tcgtccttag
    17581 cattgaatgc gattaccttt ggcagataca ttgcattagc tgcaccgtgg ataatgtgtg
    17641 cgccatagtc agcgaaagct gcgcctgtct tatgagccat tgagtgaacg ataccaagca
    17701 gagcgtttga gaatgccata cctgcaagac actgagcgtc gtgcattctg tctcttgcgc
    17761 ccatatcacc gttgtaagaa tcaatgagat ccttctggat catttcgata gcgtggatag
    17821 caagcggatc tgtatatgca cagtttgctg ttgaaacata agcttcgata gcgtgtgtca
    17881 aagcgtccat acctgtatga gctgtcagct tctgtggcat tgtttcagca aggtcaggat
    17941 caacgatagc tacatctggt gtgatctcaa agtcagcgat aggatacttg atacccttgt
    18001 catagtctgt gatgatagag aatgctgtta cctcagtagc tgtacctgat gttgaagaaa
    18061 ttgcgcagaa gtgtgctttc tttctcagct cagggatacc aaatacctta cacatatctt
    18121 caaatgtgca gtcaggatac tcatacttga tccacatagc ctttgcagcg tcgataggtg
    18181 agccgccgcc cattgctacg atccaatcag gctcaaattc gagcatgacc tttgcaccgt
    18241 tcataactgt tgttacagaa gggtctggct ctacgccctc aagaagctta acttccatac
    18301 cggcctcttt cagataagct tctgctttct ggagaaaacc gtttctcttc attgagccgc
    18361 cgccgactac gatgatagct ttcttacctt tcagtgtttt caggttctcc aatgctccct
    18421 tgccgtgata aaggtctctt ggtaatgtaa atctcatttc taattcctcc tatatgtaat
    18481 gtaataatgt gttcaaagag gtttgtgtaa atctcacaca aattctctct ttgcttgact
    18541 ttaattatac aaaatgtcaa tgaactattc taacataatc tatgaatatt ctattaatta
    18601 ttaactttta catagcatat ataagtatgt gcatacagct aaatgtacat atgaatgtgt
    18661 aaaataggtg aaaaaaatat cacaattaca tgaaaaatca cgattttgtt tgcactatat
    18721 ggaattttgc agtgcttatt tcgttttctt ttgttaataa attatacatt tgtgagtgta
    18781 gaaatattcc tgtttatgaa gataaaaaaa ttcataaaaa atttgcttaa gctattgaaa
    18841 tatgcgaaaa aatatgctat aatatattgg tattgtattg tatcctccca tgatggagga
    18901 ataataacaa ggaggaaatt taatatgaaa acaaaggtca acacaaaatc attggtgata
    18961 ctgggattga tgacagctct cacaatgatc ttctcgttca ctcctatcgg ttctatacct
    19021 atcggaccgc tggtaattac tctcaacgta attcctattg ccatagcggc tgtaacggta
    19081 ggccctgtgg gaagtgcagt cattggagga gttttcggac tgctcagctt tttgcagtgc
    19141 ttcggaatag gcgtgccaag cggaatggga gcagcacttg ttgcgatcga tccggtcctg
    19201 gctttcgttc agcgatttgt accaagactt cttgacggca ttatcgtagg atttattgca
    19261 aaaggcatga gcaaggtaac aaacaattat gtttcatatg ctgttacggg cttttttact
    19321 gcgtttttga acactgcgtt ctttatgagt gcgcttgttt tgcttttcgg caacaccgat
    19381 tatgtaaaag agcttatggg cggaaagaat atcatagtat tcatctgcac ctttgtaggc
    19441 ataaacgctc tggtcgaaat ggctgtatgt acatttgtta caggtacagt gggtgctgca
    19501 ctttataagg caaggttcat acctaacaaa aacaaagcgc aggcataaat tcaccgaaag
    19561 gataacatac aaatggtttt agcggtcgat ataggaaaca ctaacatagt aatgggctgt
    19621 tttgaaagcg acaggatact ttttgttgaa agactttcta caaatcagca gtccacggca
    19681 cttgaatatg ccataatgct caagaacatt cttgagatac attctataga tatgtcagat
    19741 ttcaggggcg gtataatctc gtccgttgtg ccttctgtta cgcttactat gaaagaggct
    19801 atggagaggc tcatcaaaaa gcgcatcatg gtggtaggac ccggaataaa gacggggctt
    19861 aagataaatc tcgacaatcc cgcacagctt ggaagcgaca gagttgcaga cgcagttgcg
    19921 gcgataaacc tctaccctgt gcctatcata atgatagata tgggcactgc tactaccata
    19981 tcagtaatag acaaggaaaa gaatttcata ggcggaatga taataccggg acttagaact
    20041 tccctcgact cgctttcggg caaggcttca cagcttcctt tcatcagcct tgtaccgcct
    20101 aaaaaggtca taggcactaa tactatcgac tgtatgaaga gcggtataat ccatagcaat
    20161 gccgcaagca ttgacggcgt tatagagagg atagaaaacg agcttggtga aaaatgcact
    20221 gtggtttcca caggcggtgt ggcaaagata atagttcctt actgcaagcg tgacatagtc
    20281 attgacgatg agcttctgct caaagggctt atgataatct acaataaaaa caaacaataa
    20341 tttaa
//
LOCUS       DAWEMR010000021        19913 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1132_length_19913_cov_11.961829,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000021 DAWEMR010000000
VERSION     DAWEMR010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19913)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19913)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19913
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1132_length_19913_cov_11.961829"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1843..1852
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7912..7927
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17851..17860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18253..18262
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5653 a         3637 c         5204 g         5373 t
ORIGIN      
        1 atagtatact tgtctgagag aggaaaatca gtaaatgaac gaaaataata tacgaaattt
       61 ctgcattata gctcatatag accacggcaa atctactctt gccgacagaa ttttggagct
      121 taccgatact gtgaagctga gagatatgga aaatcagctt ctcgataata tggatattga
      181 gcgtgaaaga ggtatcacta tcaaggctcg tgcggtgcgc cttaattatc acgctgacga
      241 cgggcaggac tatgttttca accttatcga caccccaggt cacgttgact tcaactatga
      301 ggtgtcacgt tctcttgctg cctgcgaggg tgcattgctt atcgttgacg cttcccaggg
      361 tatagaagcg cagacccttg caaacactta tcttgctatc gaacatgacc tcgaggtagt
      421 gcctgttata aacaagatag acctgccgtc cgctgaccct gaaagggctt gcacagaggt
      481 agagaacgtt ataggcattc ctgctatgga cgctccgagg atatcagcta aaatgggtac
      541 gaacgtcaaa gaggtgcttg aacgtgttgt aaaggatata ccatgcccaa agggcgacga
      601 gaacgctcct cttaaggctc ttattttcga cagctattat gaccagtaca agggagttat
      661 aatatactgt cgtgtgaaag aggggcatat caaggccgga gatactatca ggcttatggc
      721 tacgggggct gagtttcaga cggtggagat aggctatatg ggagcaactg accttgtgcc
      781 tgtgggcgag cttcatgcag gcgaggtagg atatatcgct gcttctatca aggatatcgg
      841 cgacactcgt gtgggcgata ctgtgacaaa tgcggctgag ccttgcgctg aggcactccc
      901 tggatataag aaagtaaacc ctatggttta ctgcggaatc tatcctgcgg acggagcaaa
      961 atatcctgac cttcgtgacg ctttggaaaa gcttatgctc aatgacgcat cactgtcctt
     1021 tgagcctgaa acttccattg cgttgggatt tggtttcaga tgtggattct tgggacttct
     1081 gcacatggag ataatccaag agcgtttgga gagagaatat aatcttgacc ttatcacaac
     1141 ggctccgtca gttatctaca aggtaaatct tactaacggc gaaactgtgt tcattgataa
     1201 cccgacaaat tatccagacg tggcaaatat tgcatcggcg gaagaaccga tagttaatgc
     1261 acatatttat acgccgtctg aatatgttgg aaatataatg gagctttgtc aggacagacg
     1321 tggcgtatac aaggatatga agtatataga cgaaactaga gttgacctgc attatcagct
     1381 tcccctcaac gagatagttt atgacttctt tgacgcattg aaatcacgca caaagggcta
     1441 tgcttcattt gactatgaga tgatgggcta tgcgaagtct aagcttgtga agcttgatat
     1501 actccttaac ggcgaggtgg ttgacgcact ttcattcatc gtacacgagg ataaggctta
     1561 ttcaaggggc agaagactga ctgagaagct taaagagaat ataccaagac agctctttga
     1621 gatacctgta caggcggcca tcggcggaaa gataatagcc agagaaacta tcaaggctat
     1681 gagaaaagac gtgcttgcta agtgctatgg cggagatatt acaagaaaga agaagctgtt
     1741 ggagaagcaa aaagaaggaa agaagcgaat gagacagctt ggatctgtgt cagtgccgca
     1801 ggaagcgttt atgtcggtgc tgaagctgga cgagtagaga gtnnnnnnnn nngccagttc
     1861 ggagagtgga taggttttgg tttatacaaa tatatcaaca tttttttgaa aggagcagct
     1921 atgcctgata tacaaaagtc tatgaaattg tcgctggctt tcgggctgag cggtgcggtg
     1981 atattgcctg tgctttatga ggtgtatgcc aatatctctg cggcggctgg gcttgtgctt
     2041 atcgctgtgt gggcggtgtg tgcaggagct aaattttcgg cactcaaatt caaggaagct
     2101 tttatgggca tggtatgcac acttgcttat gcaggtattc ttggcgttat ctgctatatc
     2161 gttatccacc ccaaagtttc cgatatgcta aacaggcggt cggtgtattt tcagctcagt
     2221 ttgaaacagc aggcttattt cgtgctttat gcggtgctta taagcctttg tatgttcctt
     2281 gtgtggggcg gtatatttgg tgtgaaaaag gcgatagaga gatttcggct caatcgtgaa
     2341 aaaacaggag agtatatcga caaggctttt gatgatgacg aggatatgct atgacaggaa
     2401 tttatatcca tgtgcctttc tgtaaaagaa aatgccctta ctgtgacttc tattccgttt
     2461 gcaggcctga gctttatgag gactacacgc aggctgtgtg ccgcaatatt gaggctttca
     2521 agggtaggga cataaaatgc gatactatct acttcggtgg cggtacgcca tctgtgctgt
     2581 cggctgaaca ggttgacagg ataatagata cggcaggaaa gtgctttgat ctttgccagc
     2641 ctgaaataac cttggaggct aatccaagct ctgtggacag ggagaagatg tcagggtgga
     2701 gaaaagcagg ggtaacaagg ctttcttttg gcgttcaatc ggctaatgac agggagcttg
     2761 gctttttggg caggcttcat gacttttctc aggcaaagtc ggctgtggaa acggcggctc
     2821 aggtgggatt tagacacatc tcatgcgata tcatgctcgg ggtaaagggt cagacccttg
     2881 aaagcctttg ggatagcata gagagcctta cggcactccc tgttgatcat atttcggctt
     2941 atatgctgaa aatagaaaag ggtacggctt ttgattgtga tgagatgaga aacgctgtag
     3001 ctgatgacga tatgctgtgc gatatgtatt tgcaaactgt gggacagctt gcaagcaagg
     3061 gctttttaca gtatgagata tcaaattttg ccaaagaggg cggaaaatca cggcacaatc
     3121 ttaaatattg gcacggggaa gaatatatag gcattggccc gtcggcacat tctttctttg
     3181 ataatgagag atattactgc aagagcgatg ttgaggagtt tatttctgac aaggtgcaga
     3241 gcatggtggt gcttgaggac gaccctgaca aggcagagga gtatgtcatg ctggggttaa
     3301 ggctcacgga gggcattgac ataggtaaag ccgaggagct tggtggtaaa gagcttgggg
     3361 agaatatccg caagaaggcg gctttgtttc agaaacaggg gctttgcgtg gtcgaaaagg
     3421 gtattttaag gcttacgcct gcgggttttc tggcttcaaa ctctattatc ggtgaactgc
     3481 tgtggtaaac ctgtataaat ataggcagac agtgttgtgc agttatacaa tagatatacg
     3541 atatgtaggg gataagcttg aaaaatgaca gaaaatgtgg tataattcac tgcggcggag
     3601 tgtgtccgca gttttatatt accgagaggg cttttttatg aaaagtataa gatttattgc
     3661 tttgatctca gcagcggcaa tgtgtttcgg actttgtgcc tgcggaaagt cggagaaaaa
     3721 agacgagata gctgtgccta ttctggaaac taaggatata acatataaga cggtaaaagc
     3781 tgagatagga gatatctctc aggagtatca tcaggagggc gtttatgatt acccttacag
     3841 tgaaaccgtc accttcaagg caagcggtca gataaagtct atagatgtgg agtcgccttg
     3901 tgatgtgaaa aagggcgacc tgctttgcac actttattct gacgatgtgg acgaacagat
     3961 agaagaggaa gaaataaggc tcaatcaggc gaagcagacg gtcgctacat tgcaggcgaa
     4021 cggtgctgac tacagtgaga tacagctttc tcagtatgac ttacagatag aacagctcaa
     4081 atatgacagt cttgtggcgt ccttggagga ttataaggtt tacgctccat gtgacggaca
     4141 gtttgatctt gcagacagac aaggtcagga gctgacagcg ttcatgccgg tgaataaggg
     4201 cgaggtgcta gggtatactt ccgataaatc acaaaaggct ctgtgcgtgt ctgtgtttga
     4261 taacgcgctc acaaacgtga attttggcac tgcggtgcag atagtgcagg gtgccaatac
     4321 gatgggcggc actgtgacgg atataatcta caatgagagc ggagatttct cggcatatgt
     4381 ttatgttata acccctgacg aggagaacga tatgctggat cttggcggca tagaggttgt
     4441 ctttgacgtg tattcaaggc ttgatactgt tatagttccg cagaaggcgg tgaagaagct
     4501 tgatggacgc aactatgtga acctgcttgt ggacggtgta aagatagagc aggacgtaga
     4561 gcttggcatt gaggacaacg acaacgttga gatactttcg ggactcagcg gcggtgagga
     4621 gataatactc aattgacggc attcagccaa ttaaatatta tttgatatgt ctgatttgtg
     4681 ttttaagaaa aaataaagaa ataaagcact tgattttttg cgtaatttcg tgtaaaataa
     4741 aagaacagag tgtctaggga ggactaaaaa tgaaggattt tttatctaac gtatgggtaa
     4801 agcgagcggt atcagttttc aatgtcgctt actttgctgt aataactctg ctcacttatg
     4861 caacgttcct gtatgatctg gagtttgcgg caggcagaga aaaatcgttt ttcacagtgt
     4921 atgtggtgct taacgtagtt tttatgggac ttatgctctt ttcaagacgt gagcttgtga
     4981 cggagatact gagcatactc atgcttccgg tcgtgttctg catgatactt ttcaacatgg
     5041 gcgactggat attgatagtt ccgccgttca ttgttgctat cataatgttc tttgcagcag
     5101 gcacgaacga aactgtaaag gttataatgg gtacaatata tctgcttttg tatgttcttg
     5161 gaatagttgc atactttgtg cttaatatcc ttttcggagg cacgtctgtt gaaactgtgc
     5221 taaattcaga tcttgacaca tcgtcaagtg tttatgctct ctacagggat aacttcaaga
     5281 agctcactga agtaacgtca gaatcaaata ccatatcgcc tgacggccag tatcagataa
     5341 tactttatga tgtaaaggac agcgacaagg gtgctgtaaa gatatgcgta gtgccatata
     5401 atcaggatat cgagcttaaa ttctttactc ttaagcagaa aggtatcaaa aagaccattt
     5461 ccaacaaggg tataagagga acagttcctg atgttggctg ggtaaaggag gacggagttc
     5521 tcaaggtgca gtacagactt tcggaggctg atgatctgag agctacttct gttacaacaa
     5581 tgccggataa gcagtatttc cagttccttg gtattcaata agttcgcagg accgtctgag
     5641 aaggcggtct ttttttgata aaaaatggct gtcaataaga gcttattcat aaaaatcgtg
     5701 tggttatttt ggttaaaacg cttagcttgt attacaaaac agttacatta tatgaaaaat
     5761 tgtatggtac acttgacaga tttgttaaaa aggtgtaaaa tattaatagt gttttataac
     5821 gtttcgatac ttgtgtgaac ggctatgaaa gcgtttacat acaatagtat caagggagga
     5881 taagaattaa aatgagatca aagagaatac ttggcgtggt atcagcgata atgatgcttg
     5941 gtgtttgtgc tgtgcctgct tatgccgaca gtgaggctga aatacctgaa tatagtgccg
     6001 acaagagtat ccttacaaca gatacttcag caccgtcctt gtcgtttgat atgaaggatt
     6061 gggagagata tatcaaggtg actcctgacg gtgagaagat atcagtggga gcatcccaag
     6121 aaaagaccta tgcttatcag ggtgcaagcc ttaagataac agcaacacag cctaatgaca
     6181 taagcgatta ttgtactttt gcttcgctta taagagatga cgataagaat ttggtttatc
     6241 ctggctctga ttctgaagat gcgccttata taacgatggg cgttgagatc gacgccaagg
     6301 attttggtat gacttgtttt gacggctgta tgatttcttt taagtacagg ataaatcagg
     6361 acgctcaggg caagcttatg ggagattctg tgtttgcttt tccttgtact gaggaataca
     6421 aaaaggtgac cagcaatagt gttcagcttg aaataaatac agatgcaagc aataatgtta
     6481 ctcagtatgc gacagcactt ataccagtac ctgacaagtg cggagcaaca aagttcgtat
     6541 ttgagatacc ggttataaag aaaatggata agacagatgt gctttacatt gataatcttg
     6601 taatagatac tccgttgcag aagaacgatg tagatcttca ggtaatgaat ctcgatggct
     6661 acaataaaag tgcaaaggca caggagatcg tgcagggctt aaagatacag cctaagtcaa
     6721 acggactgag cagcacttcg tcatctgagg ctgaatcttc aaagggtatc ggagtagtag
     6781 gcattgtagt tattgtggtc gttgcggtgg ttattgttgg tgctgttatt ttcgttatca
     6841 agaaaaagaa atcgcggttc tattgatttt ttgatctgac agagagatgg gcttttggct
     6901 catctctttt ttgttttttt atgaatatat cctcatctcc gagaaataat agcattagcc
     6961 taaaatggca tatcttatac tgaaaggaac ttaattatga aaaaaatgtt cgctatgcta
     7021 acttgtctcg gtctttgcgg tgttatgctc gcaggctgtt caagaaatga cgataactca
     7081 ggttcgacat cttccggcgt tactgcgacc acaacttcta aaaatgaggt cacaagcaga
     7141 cgtgatgaca gcgacacgct tgtgagccgt gttggcgatg gtatcaagga cactgtaagc
     7201 ggtggtgcgg atattattga tgatactgta agcactgtag aaagcgttgt tgacgatatc
     7261 atcagataag ctgcacatat gcttgcggac tgctcagccg tgatattatt tcggcttgcg
     7321 gcagtccgtt ttttattgtg aaatcgtatg ataaaactca aacaatgtca aaagtcaatg
     7381 gttgactatt gataaattat taacgccaaa tcataattaa ctaattattt gactaactgt
     7441 gaacgaagtg taaaatccat gtaaaataaa tgtttaagtt tgcggtttgc tctttaaaaa
     7501 tgtggggctg tgtggtataa tgaaatacat caggcagata ttttctgcta ttaagaaaat
     7561 gtaaagattt atggtggatt tctttatgaa aatcggagaa aataagtgaa aaatataggc
     7621 tctgagcttg gcggcttgct gaaaaaaata tgcttatggg gcggtgttat ggctgttgta
     7681 tgctgggcat ttactttgct gtggggcttt gacatcacac agcttgcggg ctttgcattg
     7741 ggctggggct atatgtgcct ttgtatgtac taccttggtg tgacctgcga aaaggttgtg
     7801 gagcttgagg gcaaaaaggc taagcggaca atgcttgggt gttacatgac taggtatgga
     7861 gtgctttttg ttctttgtgc gggggctatg ctcacgaagg cagtaagtcc annnnnnnnn
     7921 nnnnnnntgt catacagata agagaaagaa gggataaata aatgacgggt ataggaaacg
     7981 gccctaagat agtgtttgaa ataggcagtc tgaggatcac cgaaacggtg gtgatgggtt
     8041 ggcttattat agttgcggtg ctgatactgt gcttgtggct cacacacgat ttgaaaaaag
     8101 tgcctgagaa aaaacgtcag gtggttgcgg agatgcttgt gaacttcgtc aacaacatgg
     8161 tcaaagagaa catgggcgtt aagatgatga agtatgcccc ttatattgct acgctgctta
     8221 tttatgcggt gtttggcgct cttatcagta tgataggtct gaggtctatg actgcggaca
     8281 tcaatgttac ggcaacgtgg gctcttatga cattcgtgct tatcacatac aataagataa
     8341 aagcgaatgg tttcaagggc tatcttaaaa gctttgctca gcctgtggct tttatcctgc
     8401 cgctcaatat catcagtgag gtggctacac ctgcgtctat ggcgttccgt ctgttcggca
     8461 acgttgcagg cggcatggta ataacgggac tgctttatgg tgcgctcggc tcactgtcat
     8521 ctgcactggg tctgactttt gaggcaggct catgggcgtt cagtgtattt caggtaggta
     8581 tacctgcggt gcttagcgtg tatttcgacc tgttctcagg gtgcatacag gcttatatct
     8641 tctcaatgct caccatggta aacgttggtg cggcggcaga aacagagtaa attaccgctt
     8701 ttgcggaaag tataaaaaat gtaaataacg gaggtttatc attatggaaa aggctattct
     8761 tcttggctgt tcagcaatcg gtgcaggact tgcaatgatc gcaggtatcg gacctggtat
     8821 cggtgagggc tacgcagtag gaaagactat cgaggctatc gcaagacagc ctgaggctaa
     8881 aggcgactgc acaagaacaa tgttcatagg cgttgctatg gctgagtcaa caggtatcta
     8941 cgcattcgtt gtagcactta tcctgctctt tgctaaccca tttatcggaa agctttaata
     9001 aaaagcttca aacaagattt gctctgcatt ggtgcggagg ggaggttgga taaatggtat
     9061 tggcagcagg cgatgtgaca gagtttctgt caatagatct gacaacgata attgcgactt
     9121 tgctcaatac gctcatactg tttttggtgc tcaagcattt tctgttcgat aaggtgaaca
     9181 aggtcatcga ggacaggaaa aatgatgtta aggaaactta cagacaggct gacgaggcaa
     9241 aagaaaatgc cgagaagctg gaagctgagt atacaagcag attagagggt gcaaaggaag
     9301 agtctgcaca gattatacag acggcaacaa gaaaggctca acagcgttct gacgagataa
     9361 tcagcgaagc gagaactgag gcaaagggtc ttgtggacaa cgcttcaaga gagatcgaaa
     9421 gagaaaagaa gatcgctgtc aaccagatca agaacgagat caccgatatt gctttctcgg
     9481 ctgcggcggc agttgtggaa aaggatatga caggcgagga caacgaacgt cttatcgaga
     9541 agttcattga caatgtgggt gagatgtaat ggcagacagt atcgggaata tttacggcaa
     9601 cgcacttttt gaggtgtgct gcgaggaaaa ctgccttgat gaagtctatg gtgagcttgg
     9661 gagcataagg gatatacttt tctctcaggg tcaggaggaa tatgtggagc tgcttgcgtc
     9721 accgctggtg acggagaacg aaaaaagggc gacgctggaa acggtgttta tgggcaggat
     9781 acagcctgtt acgcttaatt ttctttgtct tgtgagcgaa aagggcagga taaggtttct
     9841 gcctgagata tgcgcaaagt tcaaggagct ttatgaaaag cataataata tcatagaggt
     9901 cactgcggtg acggctatgc ctttgtctga aaggctcagg gacaagcttg tggttaagct
     9961 ggaagctgtt tcgggggcaa agatagtgct tgtggaaaag gtggacaaaa gccttatggg
    10021 cggtatcgtg ctgagatatt cagacacaga aatagacggc agcgtcagaa ctaggcttga
    10081 tgatatcaag gctcagatcg acggggtcat tgcataggaa aggaacggta ttttaacatg
    10141 aaattacgtc ctgatgaaat aacaggcttg ataaaacagc agatcaagga ctaccgcagc
    10201 aagcttgagc ttgacgatgt ggggacggtc tgcactgttg gcgacggaat ttcaagagtt
    10261 catggacttg acaagtgtat gtcgggcgaa cttcttgaat tttctaacgg aacatacggc
    10321 atggcaatga acttggagca ggattttgta ggctgtgtac ttctcggctc tgaggacgga
    10381 ataaaagagg ggtctggagt aaagaggaca ggcaggatag tttctgtgcc tgtgggagat
    10441 gcgcttctcg gacgagttgt gaacgctctc ggtgcaccta tcgacggcag gggcgctgtg
    10501 cttacaacag aaacaagacc tgtggaaagc cctgctttcg gaataattac aagacaatca
    10561 gtaaacagac cattgcagac aggtatcaag gctatcgact ctatgatacc tataggcagg
    10621 ggtcagcgtg aacttattat aggcgacaga cagacaggta aaacttctat tgcacttgac
    10681 actatcataa atcagaaagg caaggacgtt atctgtatct atgttgcgat aggtcagaag
    10741 cagtctactg tggcaaacgt ggtagatacc ctttctaagc atggggctat ggattattct
    10801 atagttgtat cggcaacggc ttctgaaatg gctcctttgc agtatatagc accatatgca
    10861 ggcgttgcaa tggcagagta tttcatgttg cagggcaagg acgtgctgtg cgtttatgac
    10921 gacctttcaa agcatgctgt ggcgtacaga actatgtcac ttctgcttca aagaccgaca
    10981 ggcagagagg cttatcctgg agatgtattc tatctccatt caagactttt ggagagagcc
    11041 tgctgtctta acaaggacta cggcggaggc tctataaccg ctctgcctat tatcgaaact
    11101 caggcaggcg atgtttcggc ttatatcccg acaaacgtta tatctatcac tgacggacag
    11161 atattccttg aatctgagct gttcttctca ggcgtcagac ctgcggtaaa ccctggtata
    11221 tcagtatccc gtgtaggcgg ctcggcacag ataaaggcta tgaaaaaggt ttcaggctcg
    11281 ctgaaactgc tttattctca gtatagagaa ttgcagtcgt tctcgcagtt cggctctgac
    11341 cttgacaagg acactaagga aagacttgca cagggcgaac gtatcgtgga agtgcttaaa
    11401 cagggcaaga cctcgccact ggacgttgag aagcaggtgg taataatcta tgcggtggta
    11461 aacaactatc tgaagaatat cagagttgag gatatcgctg aatttgagaa agatcttttt
    11521 gtgtatatag atgaaaccca ccctgacatt ttctcttcaa taagagaaac gggggagctt
    11581 acgaaagaga atgaacaggc aataaaggac gtgcttgcgg agtattcaca gaagtttctt
    11641 caaagcagat aaggagcgct gagcaatggc aacagctaat atgaaagacg tcaaaagacg
    11701 tatcaaaagc gttgaaagca ccatgcagat aacaaaagcc atgcagcttg tggcggcttc
    11761 taagctcaga cgtgccaaag acaaggcggt ggcagttcag ccgttctttg aaacgctttt
    11821 taagacaatg tgcgatatat cctgcgacag agctttcaag tcggtgtata caaagaagaa
    11881 gttccttaat tccacactgc ttatagtggt ggcaggcgac aggggacttg caggaggatt
    11941 taactccaac gtgctgaaaa tggctaaggc aaaggctgac gatgtgctgt catcaggcgg
    12001 tcagcttcgt gttatggctg tgggcaaaaa ggctgttgag ttctttgaaa agcgtgacta
    12061 tgaccttgtg tggaaatatc cgcagatagc ggagagcatt gacactgaca ctgttataga
    12121 cattgtggaa gatattatcg caggcttcag aaatggggat tatgacaagg tagagcttgt
    12181 gtataccaca ttcgtgtcgg cgctgacaca ggagccttgc agtttgcaga tacttcccgt
    12241 ggaggatctt gacaagcttg ggggaagaaa gcacccgtca actatctatg acccgtcccc
    12301 tgaggaggtc tttgacggta tgataccgca gtatataggc ggtatgcttt attctgcgat
    12361 agtggattct tacgcttctg agcaggcggc aagacgtact gcaatggagt ctgcaacgga
    12421 caacgcagga gaaatgatag acaatctgtc gcttctttac aacagagcaa gacaggcgca
    12481 gataactcag gagcttacag agataagctc ggcaactatc ggaagatagt atttaaaaga
    12541 tgtgacggct atgatgatag cttatatccc tttttgaaag gtggtaaata aatggaaagc
    12601 aaaaacatag gcaaggtagt ccagatagtt ggtgcggtag ttgatatccg ctttgaaaag
    12661 gacagcttgc ctgatctgct caatgccatt gagatagaca acaagggcga aaagctcgtt
    12721 gttgaggtgg ctcagcatat aggcgacgat atagtaagat gtatcgccat gggctctaca
    12781 gacggactta tgagaggtat cgacgctgtt gatacgggca aggctatttc tgttccggtg
    12841 ggcaaggaaa ctctttcgag aatgttcaat cttctgggcg agcctgttga caatctgcct
    12901 gcccctgaaa ctaaggagcg ctgggagata cacagagaac cgccgacata cgaagagcag
    12961 tctgcttcaa acgagattct ggaaaccggt atcaaggtca tcgaccttat cgcaccatat
    13021 ctgaaaggcg gaaagatagg tcttttcggc ggtgcaggag taggcaagac ggtgcttatt
    13081 caggagctta taaataacgt tgccaatcag catggtggta tctcggtatt cacaggcgtt
    13141 ggagagcgta cccgtgaggg caacgacctt tattatgaga tgaaagagtc gggagttatc
    13201 gataagactg tgcttgtgta tggtcagatg aacgagccgc ccggagcgag aatgagagtt
    13261 gcactttcag gtctgacaat ggcggagtat ttcagagatg ttgagcatca ggacgtgctg
    13321 ctttttatcg acaacatatt caggtttact caggcaggtt ctgaggtgtc ggcactcctt
    13381 ggcagaatgc cgtcagccgt tggttatcag cctacccttg caactgaaat gggtcagcta
    13441 caggagcgta tcacttcaac aacaaaaggc tctatcactt ccgttcaggc agtttatgtg
    13501 cctgcggacg atttgacaga ccctgcgcct gcaactacgt ttgctcacct tgacgcaaca
    13561 acggtgcttt caagaagcat ttcatcactt ggtatctatc ctgccgttga cccgttggag
    13621 tcaaactcca ggatactttc gcctgagata gtgggcaatg agcattatca ggtggcaaga
    13681 caggtccagt ctatcctgca gaggtatgtg gagcttcagg atatcatcgc tatcatgggt
    13741 atggacgagc tttctgacga ggataagctc actgtatcaa gagcaagaaa gatacagcgt
    13801 tttctgtcac agccgttctc cgttgctgag cagttcacag gcatgaaggg caaatatgtg
    13861 cctatcaagg agactatcag aggcttcaag gagataatcg agggcaagca tgacgatctg
    13921 cctgaatctg cttttctgtt cgtgggaact atcgacgagg ctgttgaaaa gggcagacag
    13981 atcgcagaaa gtgaacagta gtaacagaac gtttaaagaa aaatacttga caagagtcgg
    14041 attatagtat atagtgaatg tatgtggcaa ttttgtgccg catgcgctga tttgtaaaaa
    14101 acttatggag aaagattatg acgccttttg atcttaagat aataacccct gaaaaaattt
    14161 tttttgaggg aaatactgag cagatcattg tgaggaccgt atcaggtgat atcggtatac
    14221 ttgcaggtca tgcgccatat gtggcaaata tcgtttcttc tcctttccgt atcaaaatag
    14281 aaggtgagtt cagaacagct gctatttcgg gagggcttat aaaggtgtct gctgacggtg
    14341 tgacagtggt gacatctgct atcgaatggg ctgatgagat agacgttgca agagccgaaa
    14401 aggcaaaggc tgcggcagag aacaagctca gagaaaatgc tagccagaaa gagtttgaaa
    14461 gggcggagca gaagctgcga cgtgccatga acagacttgt ggtggcagga aagaaataat
    14521 tatcaaagtc agagccttgc gggatatccg tgcaggcttt ggctttgttt tatttattaa
    14581 gtaaaatcgg cgtaaaggta aggtaagtaa tggggtgcgc aggggcgtgc tcggacgttg
    14641 tgcagtggaa aaaacaagaa aaggaatgaa ttgatttgag tatctttaac attatcggaa
    14701 tggcgggcgg acttggtctg tttttatatg gaatgaccat attgagttcg ggacttgaaa
    14761 aggcttcggg cggacttatg gagaaggtcc ttgcgaaaat gacgggaaac gtgttctcag
    14821 gaatactttt cggtgcgttg gtgacggcag cggtgcaatc atcgtctgca actactgtta
    14881 tcgtggtggg acttgtaaac gcaggccttt tgaaactaaa gggcgcagtt ggaataatca
    14941 tgggtgcaaa tatcggtact accatgacaa cgcagatatt aaggctcaca aacctcgagg
    15001 gcgacagcag cggccctgct atattgcagc tttgcaagcc gtctaacttc tcgtctatac
    15061 ttatcgttat cggtcttatc atcataatgg tgggaaagaa gaacaagaca aagaacgtgg
    15121 gcgagataat gatgggcata ggtatcctct ttaccggaat gatacttatg caggagaaga
    15181 tcgctccgct ggcagagctt ccacagttcg agcagctttt tgcagtgctt aaaaaccctg
    15241 ttctgggcgt attggtgggt gcgatattca cggctatcat acagtcatcg gccgcttcta
    15301 taggcatttt gcaggcgctt tcagtgtcag gcgctatcac attcgcttct gcatttccta
    15361 tcataatggg tactaacatc ggtacttgtg ctacacctct tatttcaagt ataggtgctt
    15421 caaagaacgc taagagagcc gctatgatac acttctattt caaccttatc ggtactataa
    15481 tctttcttat cggcgtttat atcattcagt atacaatagg acttccgttc tggaataaga
    15541 gctttacaac gggaagcatc gcaaacttcc atactatctt caatgttgtg gtaactataa
    15601 tctttctgcc gttctataca gtgcttgaaa agcttgccga atggactatc agagataaga
    15661 agaacgctga ggacgatgat actttcacta aggaggatct tcttgacgac agatttcttg
    15721 ttactccaaa cgttgctatc gcacaggcta cagaggcagt tgttcaaatg ggcgtgctgg
    15781 cacagaaaaa tttcatatcg gtaagagagc tttatgacaa gtatgacctt aagtctatcg
    15841 acaagataaa agagcgtgag gagcttatcg ataggcttga ggacagagta ggttcatatc
    15901 ttataaagct caacgactgc ggtctgaacg aggatgagtc gagaactgtt acagcgcttt
    15961 tccaccttat ttcagagtat gagagaatag gcgactacac tataaacatc tatgaaactg
    16021 cggatgttct ttatgaaaag gagatcggtt tttctgagca ggcgaagcac gagcttgacg
    16081 ttgtgtgcaa cgccattcag gagataatcg ggcttgctat cgaagccaca aagacagggg
    16141 atatgaacgt tctcaaggag atcgagccac ttgaagaagt agtagacaga cttgttgagg
    16201 agcttaaggc tgttcatatc gacaggtcta agaatggtct ttgcaacatc gaggtgggtg
    16261 tgaacttcct tgatatactc acaaatgttg agagaatatc agaccactgc tcaaatattg
    16321 cggtctatct tattgcaaac caagagaaat acaacagcct taaaaagcat gaatatcttg
    16381 ataagcttca cagaaacggc cctgactatt acgagaagct gttggctgaa tattcagaaa
    16441 agtttgcgct ttagttcagg tttttgcacc tgtggcgtga tataatattg taaaacagca
    16501 aggagggaca ttagtccctc ttgttataag ctgaaaagga gagagcaaaa tggacaaatt
    16561 caattacact atgctgtgcg atttttatga gctgacaatg ggaaacggct actttgtaag
    16621 cggtatgcag gaaaagatat cttattttga tctgtttttc agacagatac ctgacggtgg
    16681 cggatttgcc attgcctgcg gtctggagca ggtcataaac tatatcgaaa atctgcattt
    16741 tgacgaagag gatatagagt tcctgcgctc aaagggcatt tttgacgaag gcttccttga
    16801 atatcttaag gatttccgct tcacaggaga tatatttgct gtgccggagg gcactgttgt
    16861 gttccctaac gagcctatca taacggtgag agcacctgcg gtgcaggctc agcttgtgga
    16921 aacttatctt ctgctttgca taaatcatca gacacttatt gcaacaaagg ctaaccgtat
    16981 tgtaagggcg gctcagggca gggcggtatc tgagtttggt tcaagacgtg ctcatggtgc
    17041 agacgctgcc atactcggtg caagagccgc aggcataggc ggttgctcag gcacagcctg
    17101 cactatcaca gaccagcttt tcggctttaa tgcaacaggc acaatggcac attcatgggt
    17161 gcagatgttt gacagcgagc tggaggcttt caagacatac tgcaagtgct atccaaaggg
    17221 cactgttctg cttgtggaca cttataatgt gctgaaaagc ggtgtgccta acgcaataaa
    17281 ggcttttgac gaggtgctga agcctatggg ctgccgacct gtgggaataa ggatagactc
    17341 aggagatata acttatcttt ccaagaaagc aagaaaaatg cttgacgagg caggctatcc
    17401 tgactgcaaa atatgtgcct caaacgccct tgacgagtat cttataagag atatgatata
    17461 tcagggcgca aaggtggata tgttcggagt gggcgaaaga cttatcaccg caaagagcga
    17521 acctgttttc ggaggagttt acaagcttgc ggctgtggag gacgatgatg gcaatattca
    17581 gcctaagata aagatttctg aaaacgtggc gaagataacg actccgcatt tcaaaaagct
    17641 ttacagaatt tatgataaga ataaccacaa ggctgtggct gacctgctgt gtgttcatga
    17701 cgaggtgata gatgaaagca gaccccttac gctttttgac ccacagttta catggaagaa
    17761 aaaggtcgtc aaaaactttg aggcaaagcc tattcaacag cagatattca aggacggcaa
    17821 gtgcgtatac aagaagcctg catatgagga nnnnnnnnnn atatcgtaaa gtatgcggca
    17881 tctgagatag acacactttg ggacgagatc acacgatttg aaaatccgca cacttattat
    17941 gttgaccttt ctcagaagct ttgggaagtg aagaattatc ttcttgcgga gaacaacaga
    18001 caaacggcag attaaaattt acattattga cttgaaaaaa gctataggct gtgttacaat
    18061 aagcgtggtt agcaaattct aacctattaa gcattggagg gtcagaaatg gaaaagataa
    18121 cagtaaaaat agacggaatg atgtgcggaa tgtgtgaatc gcatatgaat gacactttca
    18181 ggcagaattt caaggtgaaa aaggtaagct cttcccatgc aaagggcgaa actgtggcta
    18241 tagttgatga ggnnnnnnnn nngatatccc tgatgaaaag cttaaagaag cagttgagaa
    18301 aacaggctac acttttattt ccgctgaaag agagccttat gagaaaaaag gattttttgc
    18361 aagattcaag taaatagcta tgcacaaagc cgtggcagat gaaaaggtct gtcacggctt
    18421 ttttgtttgc ttgacaaaac atattttctg tgatataata aatatcagtt agtatacatt
    18481 aactgatgtt ctatactttt gatactgcac aagaagggtg cggctaagtt tgcaaaaagc
    18541 tttttgtgaa aaggcatttt gtttcgatag gcttagcttc gtggcgtgca gtaattttat
    18601 agatgcaagt tagctatagc taacaaagaa aggggcatac aaatgggaaa cattcacacg
    18661 tctggggagg actatctgga gacgatactc gttttatgga acaagcaaaa cggcgaggtg
    18721 aggagcattg atgttgcaag ggaactgaat gttacaaaac ccagcgtatc aaacgctatg
    18781 aagatacttc gtgagggaaa gttcatcaca atggacgaaa acggttttat cactctaact
    18841 gatgccggca gatatgttgc ggaaaagatc tacgaaaagc accttgtatt gacagagtgg
    18901 cttgtaagtc ttggtgttga cagaaaaata tctgaacatg acgcctgcaa gatagaacac
    18961 gttttgtctg acgagagctt tgcggcattg aagaagcata tcagtgaaga acaatgagca
    19021 tgactatttt taaaggcatt ctgataccgt ttttgggtac atctcttggt gcggcgtgtg
    19081 tgcttttcat gaagaaaaat ctgcattatc ttgttcagcg agccttgaca ggctttgcgg
    19141 caggagtaat ggtggcggca ggcgtgtgga gcttgctaat accctctatg gagcaatcag
    19201 cgaaaatggg tagactgagg tttgttcctg cggcggttgg atttatgctt ggagtgtttt
    19261 ttctgctttt tctcgataga gttataccac atcttcacat gaatgccatt gaaccggagg
    19321 gtgcaaaaag cagttttcag cgcacaacga tgcttgtgct tgcggtgacg cttcacaata
    19381 tccccgaagg aatggctgtt ggcgttgtat atgcaggctg ggcggctgac catcatgcca
    19441 taagcgccgc tggtgcgttg gcattatccc tcggtatcgc aatacagaac tttccagagg
    19501 gtgcgataat atctttgcca ctgcgttcgg agggtatggg aaagggaaaa gcctttgttt
    19561 acggcgtttt atcgggagtt gtcgagcctg ttggggctgt gctgacgata cttttggcac
    19621 agtttataat ccctgttttg ccgtatcttc taagctttgc ggcaggtgct atgatttatg
    19681 tggtggtgga ggagcttata cctgaaatgt cggaaaagcc acactccaat attggaacaa
    19741 tagtttttgc gctgggcttt gtgcttatga tgatacttga tgtggcactt ggataggtgt
    19801 ttttttatgc ttacataggg atcatcttta tgcatggtaa gtgtattgaa tatctttttg
    19861 tatagccggg catatcattt tgaagtgata tgcccggctt tgttttgtgt gta
//
LOCUS       DAWEMR010000022        19731 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1148_length_19731_cov_11.966558,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000022 DAWEMR010000000
VERSION     DAWEMR010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19731)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19731)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19731
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1148_length_19731_cov_11.966558"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4681..4690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6370..6379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7526..7535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5756 a         4353 c         4406 g         5186 t
ORIGIN      
        1 ctatctctct tttctttttt cttaattttg tccaatatct attgtagcac tacagttttt
       61 tttgtttgca aaggtctaaa atgcttgaca ttatgggcgt tatggtctat tattatttta
      121 tacgacttta ttttaatagg agatatataa atgagtttaa aaaacgcttt caccattatt
      181 ttcggcaagc ttatggtaaa aactctgcac ctgctgggca gggacgcagg caactttccc
      241 gggcttgtgc tgtggactat aaataaagac tgcctgaagg ctttcaaagt ggattgccct
      301 atcgttgctg tcacaggcac caacggcaag acctctgtta caaactatct cagccacatt
      361 tttgcaagcg gtggaaagaa gataatcaca aaccctgagg gtaacaatct tgatacgggc
      421 atatgctccc tcctgctcaa tcactgcgat atgaggggaa gagtccatgc cgattatctt
      481 gtgcttgaaa ctgacgagtc ccacgttcct gtggtctatt caaagctcaa tttgcagacc
      541 cttgttctgc tgaatttctt ccgtgaccag cttgacagaa acggcgaggt ggaaacactc
      601 atactcaagg tgaaaaagtt ccttgaaaca tttgagggca acgttgtcct caatgccgat
      661 gacccaaacg tggcaagact tggacttgct aaccctaata acaagaatat ccactatttt
      721 agcgttgaca gatatcaggg cgcaacggat aagccttatg aggtgggcga gggcaagttc
      781 tgtcctttct gcgatactga acttgtctat gactattatc agtattcaca tatcggcaag
      841 ttccattgtc caaagtgtgg ttttggcaat atcgagcctg aggtggagat aaagaacgtt
      901 gacctgactg ttccgtcatt tgaagcagac ggggaaacat ataagaccgc tcataacagt
      961 atctattata tgtataacat ggcggctgtc tataccgccg caaagcttta taactttgat
     1021 aaggctatcc tgcacgatac ctttgagcat ttcgaggtga ataacggcag acttgaacgc
     1081 tttgaggtag acggctcaag ccttcttgtg aaccttgcca aaaaccctgt tggcgcaaat
     1141 atgaccctgc gtgtgatgaa cgagcaggca ggctcaaagg aactgctttt tgtccttaac
     1201 gataaccttg cagacggtca tgacgtttct tggatatggg atataaactt ctctgtgttc
     1261 aataatgttg acagagttgt gacttctggt acaagagcat atgatatcgc cataagaata
     1321 aaatgctcag gctatgaccc tgacaagata ttcgtttatc ctgatcttga cgaagcaaca
     1381 gcgtcactct tctccacaaa gggtgataag ttcgccatag ccaattatac tgctatccag
     1441 ccgacaagag ccgcaataaa aagatataag agcctgaaag aaaacgggga ggaaaagtaa
     1501 aatggaaaaa ctgaaaattc ttcatatgta tcctaatatg cttgacctct atggcgacag
     1561 cggcaacgtt gaagtgcttt cttaccgctg caaaatgcgt ggcatagagc ttgaagtttc
     1621 aaagcatctt attggtgaca aggaaactga tttcacccag tatgatatca tctatcttgg
     1681 cggcggtgct gactttgaac agcagatact tgccgatgac ctcgtttcct gcaaggacag
     1741 tatcatggaa gcttataagg caggaacatt ttttctgatg atatgcggcg gctatcagct
     1801 catgggtcag tattataaag actcaaacgg cgagaaagtg ccgggattgg ggctttttga
     1861 ttactatact gtggcgtcta ccgataaaag atgcagatgt atcggtaata tcgttataga
     1921 agctcagctt gacggagaaa aatacaaggt gataggcttt gaaaaccacg gcggacagac
     1981 caccaacgtc aagtcaccct tcggaaaagt cctctcaggc aacggcaacg agtttgagag
     2041 cagtaccgag ggctatatgg aaaaaagcgt tatcgcaacc tatctccatg gtccgcttct
     2101 gtctaaaaat ccaaaggtca gcgactatgt tatttcatac tgcatgagcc gtaagcttgg
     2161 cgaaaagtat atccctgcac ctataaacga taagcttgaa gaagattgcc gcaaacagct
     2221 ttttgagagg cttggtgtgt aaaaatgttt tcactgttca aaaagaagca ggctcagagc
     2281 gaacctccgc tgaaaaagaa aataaaggat atgaagtgca gaaaaataaa ctacgttgac
     2341 gagggctttg ataccctcgc atcggaaatg tctgccgacc cgaaagctat tctcaggctc
     2401 aagcctgtga actattatgc cataaaaaat aaatatatca tgggaaaagt ttatacgtct
     2461 gaggattatc aggagaatta tgtgcagttt ttcagatacg agtatgacca cgagtgcgga
     2521 aaaactgata tctaccccct aagtgcagag cttatgtcaa aggctcttgc aaaggtgggc
     2581 ataataatag acctgaaagc ccttgccaaa gaccagtgag gaaacgaaaa tggcttcaaa
     2641 acttgacgaa tttgatatga aagcccctgt ggctgacctg ctttatccct ctgtggaaaa
     2701 gcacaatgca atgaaaaaaa gagctgccgc ccttgaaaag cttcccagag cgtttgttga
     2761 caatctggag cttgaaaagg tggctgattt tatcacgccc cattaccagt atcgtgtaat
     2821 gaggcttttt tgtgaggttt gcgacgatga ggaggtaata aactaccgcc ttgatatcct
     2881 tgatgatttt ataaggcttc cgtcattgtc tgccaccata cgcaaggtga taaacgtcat
     2941 ggtggaaaat gacagaaaga acatctataa tcttactact cccgacagct tttcacagct
     3001 tgatgatgcc ataagcggct ttgacgctta catacagtgc atggagataa tgcacgactt
     3061 ttatggaaaa atgaaagaca agatacgctc agagggcgta aaaaagcttt ttgcgttctt
     3121 tgaggagcat tacggtgaca agcattatat atccctgaag ggtgagatag agaagcttaa
     3181 aaaggcactc tatgaccgca tacgaagcgt tacagtcgcc ataaacctcg atgaaaggct
     3241 agttcctgtt tcagcaggca ttatagagct ttcaaaagag cgttacgata taaaaccgtc
     3301 ccttgttgac aggatacttt atcatggggc aaagttcaac gataaggctg ttgtgaaaaa
     3361 tctcagaaaa aagtataatg acagcgaggt gtctgacgat aagcttatca atactgtgga
     3421 taagaccctt tttgacgagc ttagcgatct tactgatact tttgtgaaaa tgatagacaa
     3481 ggtgcttgcg gagtatcaga aagcaggcat tgccgatatg cgctatatag actatcagct
     3541 tgaatactac atgggcgcag tggctatgat agaagcctgc gaagatcagg ggcttaaaat
     3601 gtgccgtccg aaaattcttc ctgcaggtga gagaaaagcc gtcataaagg atatctttga
     3661 ccctgtttat ttccgtgaag ccagaatata taatcttgac cacaaggaga aaaaatccgt
     3721 tgtcacaaac gatattacca tgggaggtga cagcgaggga ttttatgtgc ttacgggcgc
     3781 aaacaatggc ggaaagacca catttctccg tgcggtcggt ctgtgtcaga taatggcaca
     3841 gacagggctt tatgtgcctg cttcatactg tgagatttcc cttgtggact atatctacac
     3901 tcagttcccg caagaggaaa aaacaggtat cgacacaagc cgtttcacca cggagataaa
     3961 agagttccgt gaaataagcg acactatcac agcaaacagc ctgcttctta tgaacgagtc
     4021 tatccaaagc acgaccccca gggagtgcgt tgaggtcgcc tgcgagcttt tgaggatatt
     4081 ctgcaaaatg ggcgtaagag gagtttttgc aacacatctt gtggatatcg catacaagac
     4141 cgtggagctt aataaagaca gcacgctcag aacaaagctt gcaagcattg tcgccgacgc
     4201 agatgatgaa acaggagagc gaaagtataa gatagtccgt ggaatgcccc gtgacaacag
     4261 cttcgctcag actatcctta aacaatacgg catagatatg gaaacgatag aaaagcgtat
     4321 aagagagaat aaagcctgac tattacaaaa ttgtaaccat acttgaaaat ttctttgatg
     4381 tgtgatatac tgaaacatac aactatgaag acaggagata aaaccatgaa aaagatacat
     4441 agaacattga gcctttttac agtcgctgca atgtgcaccg cacttctctg ctcatgcgga
     4501 gaggtgcctg acagttcaca gaccgacagc agctcaaagg cagccacaac tacaacagcc
     4561 gacacaacgg ctgatacaac caccacaggg gaggcgagca gtacagcttc tgccacttct
     4621 gcacctgaca gcagtgtttc agacagttca tcagcggtaa cggacgatac aaagacaaac
     4681 nnnnnnnnnn aaacggtatc acacctgcta tgtggaaagt gacttcacct gagggcaaca
     4741 caatggtgat gctcggctct ttccatgcac tgaaagacga gtgctatccg ctccctgagg
     4801 cagtaacgaa tgcgtacaac aatgctgata tacttgcggt ggagtgcgat ataacctcca
     4861 caagcgagga cggcgagtat atgaaaaatc ttatgaagca aatgctttat aatgataaca
     4921 caaagctcag ccagcatatt tccgaggaag catattcagc actgcaaaca tatctcggct
     4981 attggggcat ggatatctcc gccctggagg tctacagacc atgggctgtt tcaagcactc
     5041 ttgataccct gcttattcag gattcagact ttgacacgga aaagggtctt gataacttcc
     5101 ttcttaccac tgcccacgca gacggcaagg agatatacga agttgagtcg gtggatttcc
     5161 agttgaatat gctcataaac ttctctgacg atatctatga ccttatgttc agaagctacg
     5221 agggtgaaac caaggagagc caaaagcagg ctcttgaaga cctctatacc gcatggaagt
     5281 ctggggatat cgaaaccttc cttgaggaag ataacgagga ggagcttgca ggctataccg
     5341 aggaggacaa aaagatcgcc gaggattata ataaccaaat gctctatgac agaaacaaga
     5401 acatggcaaa agctgccgag gatcttatgt ctcagggcaa gaacgtgttc tatgttgttg
     5461 gtgcggcaca ctatgcaggc gagggaggaa taatcgacct gcttgaaaaa gacggctaca
     5521 cagccgagcg tgtgcagtat tgatggatct atagatttgt ataggggaaa ggggtaaaaa
     5581 ataaatgcca cattcatcag ggggaggctc tcacagcgga gggtcacaca gtggctcagg
     5641 cttttccagc tcttcatcaa gctcgggagg gtcgagtgct aaacacataa agcacacaag
     5701 ctttcctggg gcaagaagat acgtatacta tgagaactat cgtcctgtct atgtctatgc
     5761 tgactatgat attcgcaagg ggaccgcttc aggtaagttt ttcagtatac ttggcagtgc
     5821 tgtcagcctg ctctttggta tcatgcttct tgccatgtgc tatgataagc cacaaaaaat
     5881 ggatacaagt ccttcttata gctgtgagat agtggactgt gcaggggtaa ttgacgatgc
     5941 cgatatggtg caaagcgcaa tagatgattt ctatgacgaa acgggtatac ctgttgaggt
     6001 aatgacagtg aacaacgagg attggcaggg aagctattca aagctagagg actttgccta
     6061 tgatatgtat gtgacggaat ttgacgacga ggagcattgg ctggtggttt attctgaacc
     6121 tacatctccc gaccctgatt ttaacgattg gtactgggag ggtatgcagg gcgatgatac
     6181 cgacaatatc ataactgtag atgtagcaga cgatttcaaa gatcatatgc agaaatatct
     6241 taccgccaac agccgttatg atgtcagctc tgctatagca gcggcttttg atgaaactac
     6301 gccaaacgtt atggacggtc atgtagagtg gctgttcgtg atatggggac ttctcctgtc
     6361 aggtatcggn nnnnnnnnna ggtatcgggg cttatgagat atatcagacg cttatcacga
     6421 ataaaaaggc ttaccgaagc gcagtgaaat gtgcaaatga tgcaaaagag gacacctgcg
     6481 aatactgcgg aaatgtctat gttatcggca cttgtcatga gtgcccacac tgcggagcgc
     6541 cgctcagacc acataacgtg gaaacagaac agcctgtcgc agatgctgcc aaaaaataag
     6601 aaaggggtaa ataataaatg ccacattcat cgggaggcgg ctctcacgga ggaggatcac
     6661 acggaggctc gcatcacagc agccattcgc acggaggctc aggaagaagc tcacgccacg
     6721 taagaagcac ctattttccg gacgcaagaa gatatgtata ttatgatcat cacagacctg
     6781 tctatgtcta tgcagattat gacataaccg aaaaaagaag tcctttgaga tttcttatgc
     6841 tgcttttcta tctgccgttt attttattca cattttctat gtttgccgag gcttatcatc
     6901 accctcataa gcttccccaa aattatgatt ataaaatagt tgtggaggat aaagcaaatg
     6961 tccttggcaa caccgcagag cttaggaact cgctggtggc attttataac cgcaccggta
     7021 tctcacctgc ggtcataaca gtggaaaatt ccgattggca gggaaattat tcagatcttg
     7081 aaaattatgc ctatgatctg tatgtgaacc actttgcgga cgagagccat tggctcatag
     7141 tttactctac ccctgacggc tattcttcct ctgacggctt tgaggattgg tattgggagg
     7201 gtatgcaggg caatgataca gatgatgttc tgacaaaatc agtaacgaac agcttcaatg
     7261 acgagcttca gaaaaatctc acagcgagaa cacgctatac agtgtcttcg gctatcagca
     7321 catcatttga cgacctgacg cctacagtaa tgaagagtaa agtaaattgg actatgctat
     7381 ttacttcgat cgccattctg gcatttgtgt gtctgcacgc ctgccttatg ataggcataa
     7441 atccaaaggc gagaaaatat gccaaagcaa agccgtgttc tgacgcggct caggaaaaag
     7501 cctgcgaata ttgcggctat acctannnnn nnnnnatacc tatgtcgtag acacttgtac
     7561 agaatgtcct cattgcggag cgcctatccc accagaggat cagcctgggc acgatttact
     7621 tgataaaaaa gtcaccgcct gagaagctat ttctctcagg cggttttgtt tgtcattttt
     7681 agccgtacat ctctgtctgc tcagcgtagc tgtagattat ctcggagtca gggtcaatgg
     7741 caactccctt tgcgtggaaa agctctgtta tggtgacgaa ctcgtagccc tgttctttga
     7801 gggcagggat tatcttatca agggcttcaa ctgtctgaac gtttccgttc atatcgtgaa
     7861 gcaggataat tcccccgtcc ctagcctgtg caagcacctt ttcaacacgc tcctcaacgc
     7921 ctatggcatt gttatagtcc tctgcaccgt atccgcatat aaacggcata tctataacgt
     7981 caaacattgt ctggttcacc gctatatatg gcggacggaa aaactgcgga cgttcgccaa
     8041 cagcctcttc caccttgtca gaggtaaagt ctatctcggc ctttatttcc tctgcggtca
     8101 tttttgtcat gtcagggtgg ctctgagagt ggttttcaat gtcacagccc atgttgtaag
     8161 cacgcttcat gacctctgca ctctcgtctg tgatgttgtt tccgataacg aaaaacgaag
     8221 ccacaatgcc gtgttcttca agcttgtcaa gaacaagggg agttgtgtca gtgttagggc
     8281 cgtcatcaaa agtgagggct atcacttttc cgtagtcaga cttgtcacga agctgtctgc
     8341 gcttattctg tgaactactg tcctcagcct ttgagctttc tttttccaga ctgctgtttt
     8401 cgccagtccc acagcccact cccatagcaa ggctgaaagc cacagcaccc ataagaatag
     8461 ttttaatcac ctttttcata agattttctc cttcatattt attatctcta tgatagcata
     8521 ttcccacttg attttcaata gaatttgatg ataaagcaac aaaaaaacga gcggtcaaaa
     8581 accgcccgtc aaatgtcttt tatttttcag aatgtttctt gtccttgaag aattttctgt
     8641 atattgccac tgtgataaca gcatatatcg ttcccgttag aacgcctgcg ataacgtcga
     8701 atggatagtg tacgccaaca tacagccgtg agaacgcaat gagtgttgcc actataagtg
     8761 caggcacgcc atattttttc ggcatctcca tgaacattac caccgccact gcaaaggaag
     8821 ctcccgaatg tcccgaggga aaggagaagt ccgattgtgc acctattatc ctgttaagcc
     8881 cctcgaccac ctcgtaaggt cttgttctgg caataatgtt tttgagaatg atgttgttta
     8941 taagaaaagt caccgcaagg gagcatgaac acacgatgcc tgttgttcta gtctttttca
     9001 gtatgagaag taaaacgcat atgcctatcc ataacgcacc tccgtttgca agatatgtta
     9061 tgaacttcat tatggggtcg agccaccctg cacgcaggtt gtcctgtatc cataacagta
     9121 tgtcgccgtc tagtttaagt aaggtgtcta acatttatcc tcctgccatt tatgtaaatc
     9181 taccccttga atatatttac atcaaaactg acaagcccgt caaaaaacga gcctgtcctt
     9241 tgtgtattaa tgctttagtt catattttcg caaattaata ttatgataag ttcattttca
     9301 tgtataaaaa gcttaaaatc atgtgtgata ccattaacaa attcaatttg catagaaatt
     9361 tgatttccaa ttacagctta acaggctcta tcttccagat gttttcagca tagtccttga
     9421 tagttctgtc agaagagaac ttgccggcat ttgcaatgtt cagccagcac ttcttagcga
     9481 atgcaagctt gtccttgtag tctgcgttga ccttgagctt tgcctgaaca tagctttcaa
     9541 ggtcgccaag aacatagtag tggtcaggaa cgtgccagct tgcaccgtca gtgagcgcct
     9601 tgtaaagctc cttgaagctg ccctcctcgt tggacggaac gccgccgtct gaaagactgc
     9661 catcaataag cttgtcaaga acacgcttta tcttgtcgtt tgagaaaagt atctccctag
     9721 ggtcatactt tggcattatc tcagccagct cctctactct tgcaccgaag atgtagttgt
     9781 tctcctcgcc tgcctcctga acgatctcca cgttagctcc gtcataagtt cccagtgtga
     9841 ccgcaccgtt gagcataagc ttcatgttgc ctgtaccgct cgcctcagtg cctgcagtag
     9901 atatctgctc ggaaacatca gccgcagtaa caagcttttc cgcataggaa acgttgtaat
     9961 tctgtacgaa aaccaccttg ataaggtctt ttgtgtcagg gtcagcgttc acaagcttgc
    10021 ttatctcgcc aataagcttg atgatagcct tagctctgcg atatcccggt gcagacttag
    10081 caccgaaaat aaatgttgtc ggtgtgaagt cctttataga gccgtccttg atgccatagt
    10141 aaatgtaaag tatagacaaa gcgttaagga actgcctctt gtattcgtgc agacgcttga
    10201 tttgaatgtc aaaaatagtg ttcgggtcga tgtcaacacc gtcatgctcc tttacaaaag
    10261 cggcaaggcg gttcttctga gcctccttaa cagccatgaa ctcgttgagc acagcctcat
    10321 cgtcagcata tttttcaagc tttttaagct cgtccaagtt tttcacccaa tctgcattgc
    10381 caagaagctt tgtgataagt gcggaaagct cagggttgca aagtgcgagc caacgtcttt
    10441 gtgtgatacc attggtcttg ttctgaaatc tctcaggata aagctcatac caatccttaa
    10501 gagcgtcctt tttcagtatc tcagtgtgga tagccgcaac gccgtttata tagcttgaaa
    10561 catagatagc cataaacgcc attttcacca tgccgtcgcc aacaggagaa agcttagaga
    10621 tatactctct gtcctttttg agagcgtaca tatcctttat aaaacgctcg tttatcataa
    10681 gaatgtaagt gtagacctca ggcactacct tttccacaag tctgcaatcc cacttttcaa
    10741 gagcctcagc catgatagtg tggtttgtgt agttaaagac cttgtgagcg atctcaaaag
    10801 ccttgtcaaa gtcatatcca tgttcagcac aaagctgtct tataagctct ggcacagcga
    10861 taacagggtg agtatcgtta agctggatag taatatagtc gccaaggttg tcaagagtgc
    10921 cgaacttagc caaatgcttt ttgagaatat ccgtcacaga agcggaggag aagaaatatt
    10981 cctgcttgag cctgagtatc ttgccctctt cacagttatc gttagggtaa agcaccttag
    11041 aaatgttctc agcgtcattt ttagccttta cagcaccatc atagttaccg cagtcgaact
    11101 gagcgaagtc gaaatcctcc acgctctcag cctgccaaag tctgagtgtg ccaacgttct
    11161 tagtgccaaa gccgataaca ggcatatcat aaggtacagc ataaacagtc tggtcaccgt
    11221 atttaacgac taccttgtca ttttcgcatc tcttagacca agggtcgccg tacttagtcc
    11281 agttgtcagc ttcttccttt tggaagccgt caactatctt ctgcttgaaa agaccatact
    11341 tgtatcttat tccgtagccg tccaaaggca ggtcatgagc ggctgcggaa tcgaggaagc
    11401 aagcggcaag tctgccaaga ccaccgttgc cgagagcagc gtcctctatt tcttcaaggt
    11461 cattaagact tctgccgtga gcctttaaaa gctcgtccac ctcgtcagtg ataccaaggc
    11521 aaaggagatt gttataaacg gctctgccca taaggaactc catagagaga tagcaggctc
    11581 tgcggctgtc aaggtgagcc tttctgctgt tctcccaatc gctgtcaata agctccataa
    11641 cagtagccga caggctttcg tggagctgcc aaggcagagc tttttcaacg ctcgtgctaa
    11701 acttcttttt caggtcttta ctaagttcgc tgtaaaacag ttctttgttc attggtttta
    11761 tcctttctgg tattttatgt gttctattgc attttgtact ttgtgattat ccgcacaacg
    11821 tatggtattg gtgaacattg ttcgcacgca tttatcttgc ctctcaaatt ttccgcctgc
    11881 caccacattg gatttacaca ttgttaggca aacgcaacaa actattcccc tacatcttat
    11941 tcaggcaagt tcttccgccg caaaatcgct gtcgctcttt gcgaatctac gttgcgctaa
    12001 gggatagaaa agcggtgcag gtttatgctt cgatgttggt tttatttgat gtttgggttc
    12061 tatgtgcttt ttgaatttgt tctgcaccgc ttagtaggct tacgcctatt gaggctctgc
    12121 ctcaaactcc gcaggggctt tgccccttga ccccacaagc cctctagcgt cgggcttgag
    12181 cctgcgcttt attgctcgct acgctcggtg cttgtgcaca actctgataa gctatgcaca
    12241 ttcctcactc ctcattcctc atttatcctt gctctatctg ccatacaccg ctgttatctt
    12301 cgccaatctc tccgccaaag tatcatcaag cttatcaaag ctttccaatc tccacttcca
    12361 atttgctccc actgttgacg gtgtattcat tctggcttta tcgtcaaggc aaagtatatc
    12421 ctgcatttgt gcgatagctg tatcacacac gctggaccaa caaagcctta ccattgccca
    12481 aggcacgaac tcgtcttttg taactccaag atagtccttg cagtagcttc tggtcttttc
    12541 gtcacagctc tttatccagc caagtatggt ttcgttatcg tgcgtacctg tataacaaat
    12601 gctgtttgtg gtcttaaaat tgtggggcag atagccgttt gtattgtcag aatcaaagcc
    12661 gaactccagc accttcatgc ccggatatcc gcttgcgttg agcattttta caacgtgctt
    12721 tgtaagaaag cccaagtcct ctgcgattat cttcacgtcc ccaagctgtt ttctcacttc
    12781 tttgaaaagc ctcatgtcag gaccttttct ccacttgccc tttttggcgt tctttgcacc
    12841 gtatggtatg cagtaatagc tttcaaagcc cctgaaatgg tctattctca ccacgtcata
    12901 ggtgttagaa gccgccttta tacgctctac ccaccagcgg aatttatctc ttgctatgta
    12961 ttcccagttg taaagaggat tgccccaaag ctgaccgtct gccgtgaatg cgtcaggtgg
    13021 acatcctgcc acctcaacag gctttttctc cttgtcaagg ctgaaatact gcggtgtact
    13081 ccacacctca acactgtcat atgccacata tatgggaata tcgcctatta tttcgatgcc
    13141 cttttcgttg gcataagcct ttagcttttt ccactgctgg aagtatttat actgcacaaa
    13201 acaccagaag tctatatcat ttgcatattc ttttctggca ctttttatcg ccttaggctt
    13261 gtacattttc agcggttcgt cccattcata ccaggccttg cccttgttgg caaatttcag
    13321 tgacataaac aaagcgtaat cgtatatcca gtctttgttt tccaccacga attttgagta
    13381 tgccttttca ggcttcttgc gaaaacgctt gtgtgccgtc ctaagcacct taaaaatgac
    13441 ctcgtatatc ttgccgtagt caacagaccc tttgtcatcg ccccactcaa tatccttgta
    13501 ttccgcaggt ttcagataac cctctttctc cagagcttca aggtctataa agtatggatt
    13561 tccggcgtga acagaaaagc tctgatatgg cgaatcacca tagcttgtag gagatatggg
    13621 aagtatctgc caacattttt ggtgagcctt ttcaagaaaa tccacaaaag cataagcctc
    13681 cttgccaagc ttgccgatac cataatcgtt aggcagagag gatatgtgca ttaaaatacc
    13741 cgatctacgc ataaaatgct cctttcatgt caaaaccgca cccatgtgaa aatttcagtg
    13801 cagtcacata taatacatca ttatgattat actacttttc agccattttt gcaatagaaa
    13861 aaccgccata tatttcattt tagaaatatt ttctctaaac agaaaaatcc cgaggacgca
    13921 aaccctcggg atgatctttt agtcatgtgt tatcatcata agcaccgcaa agtagtgaaa
    13981 aacgcttcct gcaatggtga acaaatgcca tattgagtga aaatatttct tgctcttttt
    14041 aacatagaaa ataacgccca cagtgtagaa aagtccgcct ataagaataa accataagga
    14101 cagaggataa actttttcta tcataggctt gacagcgatt ataactaccc agcccattgc
    14161 gatatagcat ataacagaag gcttgcggaa cttttcaagg tcaatggagt tcagcacaat
    14221 gccaagaata gccgcacccc agacgattcc gaaaagaacc cagccaaacg cacctctcaa
    14281 aggcacaagg gtgatagggg tgtaggtgcc tgcgatgagg aagaaaatgg tgttgtgatc
    14341 cattattctg aagaattttt tagcttttgg attggtgata gcgtgataaa gggttgacat
    14401 ggtgtaaagt atgataagag aagctccgaa aatagcggag cttacaacgc cccaagcgtt
    14461 ggagtaaatg gcagcgagaa cgataagaac ggcagtgccg gcaatggata gcagaccgcc
    14521 tgcaccgtgt gaaacggagt tgaagatctc ctcgccgagg gtgtatcttt tggttagatc
    14581 ctgagattgg atctcgacag attttgacat agtatcattt cctttccgat aggaccgaga
    14641 atttgcatat aagttttatt actatagtat agtaacattt ttttagacac ttgtcaagtt
    14701 taaatacagg tgtaaaaagt gaggagtgag gaatgaggaa ttaggaatga ggaattagga
    14761 atgggcaaag ctgaccagag ttgtgcccca acaccgagcg aagcgagcaa aaggaccagg
    14821 ggcacgcccc tggtcagggt caaggggtga aacccattgt gggggtctgg gggcaaagcc
    14881 cctagcggag tttgaggcag agcctcaata ggcgtaagcc tactaagcgg tgcagaacaa
    14941 attcaaaaag cacatagaac ccaaacatca aataaaacca acatcgaagc ataaacctgc
    15001 accgcttttc tatcccttag cacaacgtaa atccgcaaag agcgaagcga ttttgcggca
    15061 cagcaaactc gcccgaataa tccgtagggg cgaacatcgt tcgcccgcat ggtatgcaac
    15121 cggtaccatg taaacaaaat acccccaata acttaaatca aaaaaatttt cacccccccc
    15181 ttgacaaaac atgaatatat gttataataa acatatgaac agttattcat atgatgaatt
    15241 gaaatgaccc atatcgaata tactaaaaca ataataagaa aaggagagcg aaaataaatg
    15301 agcgagaata cagtaaagca tgagcataaa cagtgcggag gaaagaatat agagaaaatg
    15361 gaggaaatag ggaaaacaat gcccgttgag gaggagatat atgacgtggc agagcttttc
    15421 aaagtccttg gcgaccaaac aagagtgaaa atactcttta cccttataaa ggacgaaatg
    15481 tgcgtgtgcg atatcgcaga gctgttggaa atgtcacagt ccgctatctc gcatcagctc
    15541 agagtgctta agcaggccag acttgtgaag tatagacgtg agggaaaaac cgtctattat
    15601 tcagtttccg atgaacacgt cgctgtcata ctcaatatgg gtatcgagca tatcagcgag
    15661 taaagaaagt gatctgatct ttgtagtata ggagaatgtg aaatgagtaa aatgactttg
    15721 cttctaaagg aactcgattg tcctcattgt gcggaaaaga tcgagaaacg tgtgggcgag
    15781 ctttcaggcg ttaaaagctc taaccttgac tttatcaata aaaagctcac agttgatttt
    15841 gacggcgata aaaacgccct ctttacccag atagaaaacg tcgtccataa gcttgaacct
    15901 gacgttagcg tgaaagagct tggcgattgc gctgagaaaa ctctctatct gaagctggag
    15961 gatctcgatt gccctaactg cggtgaaaag atagcaaacc gtgtgggtga gctttcaggc
    16021 gttatttcat caaacgttga ttttatcagc aaaaaactta ctgtcaaaac agacggctct
    16081 aaccctgacc tgcgtatcat gatagaagat accgtccatc agcttgagcc tgacgttact
    16141 gttaaggatt atggtgctca gacagcagag gaagagcctg tgaatgacca taagggcgag
    16201 atagtcaagc tttctgtggc tatcgtgttc tttatcgcat caatgctttt ggataagctt
    16261 ggaagcggca ctgtatgtga gtatctcagt gcaggccttg caatagtcgc ttaccttatc
    16321 gcaggtcttg acgttgttat cgccgccgta agaaaccttg ttaaaggcga ggcttttgac
    16381 gaatctctgc tcatgactat cgcaacagtt ggcgcatttg cacttaaaga cttccgtgag
    16441 ggtgcagccg ttatgctgtt cttccaggtg ggcgagcttt tccagactat cgcagtagag
    16501 aagtcaagaa aatctatcac aaagcttatg gagcttaagc ctgagtctgt aacagtaatg
    16561 agaaacggca aaacatacga gcttcctcct gaggatatcc ttaaggacga gatgatcgca
    16621 gtaaaaacag gcgagagaat acctcttgac ggtatcgtaa cagaaggaga atcagagctt
    16681 gatacatcag cacttaccgg tgagtttctt ccggtagagg tcaaagcagg tgacagcgtc
    16741 ctcagtggct caacaaacct gaggggactt ctcaaagttc gggtcacaaa taccttccac
    16801 gagtctgcag tgtcgaaaat tctcgatatg gtgcaaaact ccagcgaaag aaaagccaag
    16861 accgaaaagt tcatcacacg ttttgcaaga gtttataccc ctgcggtagt aattgctgca
    16921 cttgcactcg tgtttatacc aagcttcatt ctgggctttg accagttctc aaaatggctc
    16981 tatagaggac ttctgttcct tgtaatatcc tgcccatgtg cacttgttat ctcagttcct
    17041 ctctcgttct tcgcaggaat aggcggagca tctaaaaagg gaatacttat aaagggtgca
    17101 aagaatctcg aaacaatggc gaagtgcaaa actctcgcag tggataaaac aggtacgctc
    17161 acaaagggca agttcactgt cctcagcgta aacccacagg gcgtttcaca ggatatcctc
    17221 cttgaagccg ccgcatatgc agaaagtatg tcaactcacc ctatcggtat ctccatagtg
    17281 cagagatacg gcaaggaaat agacggtgca agactttctg atattgaaga gatagcagga
    17341 aacggtgtaa gagcaaagct tgacggcaag gagatacttt gcggaaagaa agcacttctt
    17401 gaaacaaacg gcattgcagc acaaagctca gaagactccg caacggcagt ttatgttgcc
    17461 cttgacggca agttcatagg cgagatactc cttggcgacg agataaaaga aacttctgcc
    17521 acagccgttt caagacttag agagcttggc gttgaaactg tcctcctcac aggggataag
    17581 aaagataccg ccgaaaaggt ggcaaagaaa gtgggcataa aaacattctt cagtgagctt
    17641 ctccctgaaa acaaggttga aaaggtcgaa cagcttataa aagaccccga ccgcagactt
    17701 gttgcattcg caggggacgg aataaatgac gcacctgtta ttgcaagagc cgatatcggt
    17761 atcgcaatgg gtggcttagg ctcagatatc gccattgaag ccgcagacgt tgtgcttatg
    17821 aatgacgacc cgtcaagcat agcagacgca gtaaagatat cccgaaagac tatgacaata
    17881 gtaagagaga atataatttt tgcccttgga gtaaaagctc ttgtgcttat attcggtgca
    17941 ttgggacttg caggaatgtg gcttgcggtt ttcgcagacg ttggcgtagc cgtaatagct
    18001 atcctcaatg ccctaagaag cagcagaata aaataaaact atgtttagac ctgcaaatgc
    18061 gcataaatac ggcatttgct tgtaggggcg aacagtgttc gcccgcttat ccacaacaac
    18121 gtaaaaataa caaggcggac gctggaaaaa gctccgcctt ttttctgtca ttgacaataa
    18181 gcctgatatg tactataata ttggtattgt aaaaatgaac ggaggtgcag tcaggtggct
    18241 aataaagaca gacaacaaaa ctacacaaac gataataagc ctcaggacta ctacgaacag
    18301 gaatatcagc gacagcttga cgagttcaat cgcataagcg gtgcgggcgg acagagtgtt
    18361 cagcaaatag acgagggcgt gaacgacttt gacgattact atgacgctgg ctaccctgaa
    18421 gatcatacac agggtacaga tcagccatac gggcagggaa gccgttcatc atcagatcac
    18481 tcaggctcat acagagaaag cactccaaga cggcagacac agagcagaca aacgtcacgt
    18541 ccacagaacg gacagagcaa gcagagaggt tcaggtaagt cagcaaaaag caacaagacc
    18601 tcagcccaaa gacgaaataa tgacctgcaa tttggcaaaa acaataaaag ccgtaacggt
    18661 cagacgaaca aaaaaggtaa aaacaaagtg gaaaataaaa aaagacaccc tataaaaacc
    18721 tttttcaagg tgcttatcat catacttttg gtggttttca tattggcaaa cgtgcttata
    18781 tggcgataca taggcatggt gaacaaggtg gaaagaggcc agcgaacgaa cacagatgct
    18841 tccatgaaca gctctgatgt acgaaatatc ctgcttatag gctctgatac aagaaacgct
    18901 gacgagcgtg gcagaacaga ctctatgata cttctctcca ttaacagcac cacaaaagag
    18961 ataacaatga cctctttcat gcgtgatatg tatgtgaata tcaagggctt tgacgctgac
    19021 ggaaacgaaa taaacaccaa ggcaaagctc aatgcggcgt atgtttacgg cggtgcagag
    19081 cttcttatgg ataccataga gtataatttt gatattgccg ttgacgatta tgtttatatc
    19141 gactttctgt cattcgttga tatcgttgac gcagttggcg gtatcgagct tgacatttcc
    19201 gatgaagagg ccgagggcat gaagcctcca atggcagagc aaaacaagct tcttggcaat
    19261 aaaaagggta cagactatct tgataagggc ggaaagaagc tccatgtaaa cggcaatcag
    19321 gcactcgcct atgcaagact tagatatgtt ggcaacgcag attttgagcg aacagaacgc
    19381 cagagaacag ttatcacaaa gataatcgaa aaggcgaaaa gctcagatcc gctgactata
    19441 gacaagttcg caaaagcatc aatgtcacat cttacaacca atatgtccca gacacagctt
    19501 tttgctctta cctataaggc gatattctcc atgaattacg agataaagtc catgagaata
    19561 cctgatgatg attcctattc atatggcaca tcatctgacg gtcaatcaat tctcgaagtg
    19621 gattttgaag tctgcaaagc acttctcagg aaagagatat ataaccagta aaaaagaaaa
    19681 ggcgctgtaa aaaaaagcgc aacaaagccc gtggatttcc tcgatatgga a
//
LOCUS       DAWEMR010000023        19495 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1172_length_19495_cov_10.914660,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000023 DAWEMR010000000
VERSION     DAWEMR010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19495)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19495)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19495
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1172_length_19495_cov_10.914660"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15645..15654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5491 a         3804 c         4761 g         5429 t
ORIGIN      
        1 ggactttact actgcgtttc aatgcctttt tattctatat cgagatttat aatgggcata
       61 ccgcctcagc ttttttttgt atttcttctt ggcaataccg caggttatcc tgtgggagta
      121 aagcttcttt cagatctgtg tcagagaaag ataatatccc agcgtactgc aaagataatg
      181 tcctgctttt gctgctgtgg agggcctgct ttctattcgg gtacagtggg tcttgcagtg
      241 tttggttgta caggagcagg cattgcagtg ttcgtatcca tactagcggc aaattttctc
      301 actgcacttg ttctcggcag agctataaat gaggataaga aaacatcaaa gccgactttc
      361 agacttgacg gcagaatgtt caccgacagc gttatctcag caggaaagtc actttttgtt
      421 atctgcgtta tgatagtctt tttcggagcg tttatgtcct ctcttgaata ctgcggagcg
      481 ttcagctttc tgaaagatgt gttttcactg ccagaaaacg gacttgtgct tgtgaaaagc
      541 tgtcttgaaa taacctctct cactgagctt tcgggaagtc cattttatct tctgccattt
      601 atagccgctg tatgctcctt cggtggtctg tgtgtgatta tacaaatatc tgcacttggc
      661 gtggatttct cccttgtgcc gttttttatc tcacgtttgg catcagcctt tttgtctgct
      721 gtcatatgcc gttttatcta tccattcttt attcctgatt cagttttgac ttctgttaca
      781 aataatgtta aattcgtcaa agtgaacaat tttgttccct cgttttgctt gattatgatg
      841 attttattac taactttgaa gaaaagacta gcattttcta aaacagtatg atataattaa
      901 taacaaatga aaaaagatta acttatctta aaggagttgt ataaatcatg gcaaagaaga
      961 gctattttgg taacagaatt tcaccgtcat gttcttactg ccttttcggc aacagatcaa
     1021 gagaaggaaa taaggtcctt tgtgagaagc agggtctggt agacgctgat tatagctgta
     1081 agaagtgggt ctatgatcct atcaagcgtg ttccaaagaa acagcttaac attccaaatc
     1141 aggaagacga tgttatctga tacataaaat atgccgtaca ttttttggtg tacggctttt
     1201 atattgcaaa agatccgcag agcgctttgt ttcgccctgc ggatctttct atcttatatc
     1261 cttatatctt ttttatcttc gtgtaatttg ccattatctt ctttgtgcca cgattatcga
     1321 atgctatctc aacaagagta tcgttagcca taggttttga gcttaaaaca gtgccttcac
     1381 cgaatatctt gtgcaaaact ctgtcgcctg gtgcgaaagt ttcagtatta acagaaaaat
     1441 tgctcttatc agcctttcgc tgagccaatt gctgttgcag actcatgctg tgaggctttt
     1501 catagccgtt gagataaggt gaagcctttg tttcctcggc ttgcttttct atgcaatcgg
     1561 caggaagctc ttttataaag cgtgatatcc tgtttctgtc agtgcgaccg taaagcatac
     1621 gttcctctgc gtgggtaaga taaagctttt cctttgctcg tgtcaccgca acatatgcaa
     1681 gtctgcgctc ctcttccgtg tcagcttctg attcaagact tcttgatgac gggaagatgt
     1741 tgtcctccat gcctaccacg aaaactatgg ggaactcaag accctttgca gagtgcattg
     1801 tcataagcac aacatagtca gcgtcagagt cgagattgtc aacgtcagtg taaagtgata
     1861 tttcctccaa aaatcccgac agcgacggct cgtctgcatt ttcttcgtag cttgacatgg
     1921 tggatttaag ctcgttgatg ttttcaagac gtgtaagacc ctcgtcgccc tgcatttcaa
     1981 gatgacgctt gtagcctgtc atatcaagaa gcttgtccaa aagctccgca agtggcagag
     2041 tgtcagaaag ctcagtaagc tctgagagca tatcgcccac aggcataagc tgttttgaac
     2101 gcttcacaag gggagcaagc atatcggctt cctgcatgat gtggataggt gaatccccaa
     2161 ggtctgaggt tatctgctca agagcgttta cagtggcttc accgatacca cgtttcggct
     2221 cgttgattat tcttctcaga cgtagaaagt caaaatggtt gtttattaca gaaagataag
     2281 cgaggatatc ctttatttcc tttctgtcat agaatttcag accgcctatt attttatatg
     2341 gtattccact ttgtatgaac gtctgctcca aagcgttcga ttgtgcgttc attcggtaga
     2401 gaatggcatg gtcgttgtat tttctgccct tgtttatgtc ctctaaaatg gtgtcagcca
     2461 cgaattttgc ttcactgcgc tcgcttgaag ccttgtatac tgttaccttt tcaccgtcac
     2521 ccgaagctgt ccacagattt ttgccctttc tgccctgatt gttgcttata agctgatttg
     2581 cgcagttaag aatgttctgg gtcgagcggt aattctgttc aagctttata acatcagtgt
     2641 cagcattgca gtcgaactgc tcctcgaagt tgaggatgtt ctcgatagtc gcaccacgga
     2701 acttatagat actctggtcg tcatcaccca caacgcacag attgtggaat ttctgtgaca
     2761 gcagcgaaac aagtctgaac tgagccttgt tggtgtcctg atactcgtct accattatat
     2821 atttatacaa gttctgatag tggtcgagga cgtcaggaaa ctgctcaaaa agcttgactg
     2881 tgtggcagat catgtcgtcg aagtccatag cgtttgcggc tttgagcttc ttctgatagt
     2941 gcttgtatac cttgcctatg gtcagattac gataatctcc tgcggctgag gcgatgtatt
     3001 catcaggctc ttggagcagg tctttagcgt gggatatctc gctgagtatc gttctcggtg
     3061 gaaacatctt ctcggatatg tcaagctctc tcaatatgct ttttacagtc ctctgagaat
     3121 cgtcagaatc ataaatggtg aaactttttg aaaatccaag cttgtcacat tctcgcctga
     3181 gtatccttgc gcaggcggag tggaaagtcg ccgccgttat gccttgacct gcctctccca
     3241 acattcctgc aagacgttct ttaagctcgc ctgccgcctt gtttgtgaac gtgatagcaa
     3301 gtatgctcca cggctttatg cagtcatatg ccacaatgtc tgcgagggta gaagcgtcat
     3361 ttgtcttgcc gtcaatgtag tctttgagaa actgtatgtc ctgttcagag ggcgtgccgt
     3421 aggtctgctc tgtgttgtat gcgttgccga agtatatcat gtttgcgatg cggtttacca
     3481 aaacagtggt cttgccgctt cctgctcctg ccagtataag gagaggtcct tttattttga
     3541 atacagcctt gcgctgttgg tcgttcattc gtgaaaaata tttttcaagt gcttttcttt
     3601 taagagccgt aaactgctcc attatataca cctccgtaaa atttctttta tgccgtatcc
     3661 tacattatag catatttttt ctatcttgaa aagggcggcg gcaaattttt ttcacatgaa
     3721 taacagtggg tatgttttcc aataatacat taaatcaaat attatgaacg gaggaacata
     3781 tatgaaagct gttgttttat gcggaggacg tggagaaaga ttgatgccca tgacagacag
     3841 acgacctgcg gctcttctga gattatgcgg aaaagaaatt ttgcttttta ctcttgaaat
     3901 gctggagaaa gcaggctttg aagaagctgt ccttgcggtg ggctatggct ctgaacaggt
     3961 agaaaggctt cttgatgaaa aatactcagg taagataaag ctccatatga taaacaccgc
     4021 aggcaaaagc actgtgcagg ctgttagaac agcaatgtgc gatgaaacag aaattcttgc
     4081 cgtggaatgt aactgtatct gcactcaccc tcttgatgag attataaaag ttcatttgag
     4141 ccacgatacc ttttgtacgg ctctgactta tgatactgaa aacaaacctg ctggcatata
     4201 tatagtgaaa agagagcttt ttgaaagtct taatcctgaa aagcctacgg atatgacgga
     4261 ggatattatc cacgaagctg tgaaaagtgg cgaagctgtc ctgcttgacg gaaaaggcta
     4321 ttataaaagg ataaccacgc ctgaggcttt tcttgattgt caaaggcata tgctttacaa
     4381 cgaaaatatg tcacaaaggc tgacggaaaa taatttcagc ggtgcggcaa taggtgagcc
     4441 tgtttatata ggagagaacg tttctgtcat gtcagggtcg gtcatagaaa gcggaagcgt
     4501 catagacaat aacgctgttg taaagggcgg caaggtgaac ggatatgttg gcataggctc
     4561 ggtggtatct gaacgctgtg atataaacag tgcagtggta tgccgtggtg ctgtccttga
     4621 cagcggtgtg aagtgcggtg aatattccgt cataggcgaa aaggctcata tcgcaagtga
     4681 agccgtcatt gaaaaaggag tgggcatatg gtctggaaaa actgttgaaa agggcgtaag
     4741 gctctatgaa aacgtgaaaa gatcatctga cagccgactt gtcatagacg aaaacggcga
     4801 gtgcagtttg tggggcggtg aagccacagc gcaaaaggct atgctttttg gactttgtgc
     4861 cgcaagcgct gcaaaaaaag gcggaagtat agtgacagcc tatggctctg acgaaagcct
     4921 tttgctcaaa caagcccttg actgcggcat acgtcaggct ggagtgaacg tttacagtat
     4981 aggcagagca ggcatttcag agctttctta tgctgtaaac agatttggcg gagagtttgg
     5041 catactcatt ggcacaaaca tttcaggtca tgcaaggctt atttcggcag ggggaatgtt
     5101 gcctgatgaa aagcttcttg accgcattgg aagcatagct gacgacagag cttatcgcag
     5161 tgtggggctt tctcagacgg gtcgtgtgac agatttttca gcggtgaggg agatatacgt
     5221 cgcagagctt gaaaaaatac tccctgaccg atttaaaggc gtaaatgtgg atatccgcac
     5281 atatgatgtt aaaaaggcga ccattgccga cagactcttt cacggcagga acgatataga
     5341 cggtgagcgt ataatattca atctttccgc agacggcaca aaagtatcag cttacagtga
     5401 gaaaacaagc tatgtcatgt gggaaaaact ggtggcaatg tatgcagggg tgtgctttga
     5461 aaaggggctt gcggtggcat tgcctgagaa tttcccctct aatgccgatg ccactgctga
     5521 agtccactgt ggcaggcttt accgatacga aaacaatgct gacatagcaa aggacgttgc
     5581 cgtttcaacc cataatatgt ttgcctatga cggactttac cttgcgtcag cggtaacgtc
     5641 ttatctgtca gagcagggga taacgctaca aaaagctctc tgtgatgtgc ctgacgcata
     5701 cacttcaagc cgatttgtgg gcataacaat gagccgtgaa aacaaggaaa agatattttc
     5761 agagcttggc tgttcggtag agggagagat aacaatgggc aagacacacg cagttatcag
     5821 accgctgagg gataaaaaag gtataacagt gtttgcagaa agcgtgagct gcgagcaggc
     5881 ggctgccttt tgcgaggata taacaagcag gataaagggc atatttagat aaatgtaata
     5941 aattgctatt gacattgaga cgaaaatgga gtacaatata atcagatatt tttgttcgga
     6001 caaaggcggc tcatgccgct gtatttaaac tgcgttcatc acaccatttt gtctttcttt
     6061 gaggctttaa gcgtttttga cagcacatga taataaagcc caaacgcagc ggtatattga
     6121 atctaaacgg aaaaatcgaa tattatttga gcatgataaa atgcagcagg aaggagataa
     6181 ttgctcctgc cgtatgtttg tgttgctttt ttgcggcatt ttgtgccttt gaaggcttaa
     6241 aagcggagta cagcagggca aagtacatta ttttatgaac gaaaagaaca acacagtaaa
     6301 aaatcctaca caaaaacgtt tttttagaaa aaaacagaac aagagcgaga tacctctggc
     6361 tcaggcttca aaagagcttg accgtgtaaa aagtcagaga gattcgggca gtgtgaaaaa
     6421 aacgcttcag ccgagaaatg aaactccaca gagaaaaccg cagaatactc agcaaagacg
     6481 tccaagacct gcggcgaaca gagtgaaaga gcctgtaaga agaacgcctg tgaagataat
     6541 ccctcttggc ggtcttaacg agataggcaa gaatttcacc gttatcgagt gttcaaacga
     6601 tatgtttgtt atcgactgcg gattggcttt tcctgacagc gaaatgccgg gagttgatat
     6661 cgttatccct gatttctcat acgtcgagaa aaatcaggaa aagctcaggg gcgttgtgct
     6721 tacacatggt catgaggatc atataggcgg tctgccgtat tttctaaaaa aattcaacgt
     6781 gcctgtttac ggcacaaggc ttacgcttgg tcttgttgag ggcaagctga aagagcatgg
     6841 acttctagga actgtaaaac tcaacgttgt aacgccaaga cagacagtta agcttggctg
     6901 tatggcggtg gagtttataa gagtaaatca ctctatccct gatgccgtgg gaatggctat
     6961 ccatacccct gctggtgtgc ttatccatac cggtgacttc aaggtagact atacacctat
     7021 cgagggcggt ataatcgacc tgcctagatt tgcagagctt ggaaacaagg gcgttttggc
     7081 tcttatggct gactctacaa acgctgaaag acccggctat accatgagcg agcgcaaggt
     7141 tggcgaaagc tttgtgaagc ttttcaacaa ggctgagggc aagaggataa tcattgctac
     7201 tttcgcttca aacgttcaca gagttcagca gataataaat aacgctgtca cacacggcag
     7261 aaaagtggct gtgtcaggca gaagtatggt gaatgttatc tcaaagggca tcgagcttgg
     7321 ctatcttaaa gtgcctgacg gtgtgcttgt ggatatcgac actatatcga gatatgcacc
     7381 tgaaaaaatg gtcatcgtca caacgggaag tcagggcgag cctatgtcgg cgcttacaag
     7441 aatgtcggca aacgagcata ggaacgttac tatcactcct atggacttta tcataatctc
     7501 ggctaatcct atccccggca atgaaaagta tgtcacaaag gtagtaaatg accttatgag
     7561 attgggtgca gaggtaatat atgagtcaat gtacgaggtg cacgtttcag gacacgcttg
     7621 tcaggaagag cagaaattgt tgctttctat cacaaagcca aagttcttta tccctgtaca
     7681 cggtgagtat aagcatctta tgaaacacag agatacagca ctttctgttg gaatccctga
     7741 gagcaatata atcattgctg atataggtaa cgttatcgaa acagatggcg ttgacatgaa
     7801 gataacagga caagtgcctg ctggaaaggt acttgtggac ggtcttggcg ttggcgacgt
     7861 tggcgcaata gttctccgtg acagaaaaca tcttgctcag gacggactta tcattgccgt
     7921 ggcaactatc gacagggctt caggagatat acttgcaggg cctgacgtgg tttcaagagg
     7981 ctttgtgtat gtaagagaga gcgaggagct tatgactgaa gctcagaagc ttcttaccga
     8041 aacacttcag gattgtcttg ataggaatat gaaggattgg aataccataa agggcagaat
     8101 gaaggacaat ctttcggact ttatcttcac aaagacaaag agaagtccta tgattttgcc
     8161 gataataatg gaattatgac cgtctgacaa cagtgcggtt gtttatagaa atgcaggagc
     8221 tttaaaatga aaagtaacaa cggattacag atcataataa gaataacagt gtgcgttacc
     8281 atgctttcgt cacttatagg cgcttttatg ctgcattcta ctccgtctca cagagagtcg
     8341 ggcagtgtgc ttatcctagt gtctgtggcg gcggctgtat tgtgggtgct tgaagaagtg
     8401 gttttccaca atcacactcg caagtatgtg gcaaagcttt ccacgatgat ctcaaagacg
     8461 gagagagatt ctctcctgaa ttttcctgct cctgctataa taatcgacag cgaaaacgtc
     8521 atcgtgtggt ataacagact ttttggcaga caggtctact ctgaggaaga ggcttacggc
     8581 attgacctca ctgagcttat gaacattgat atggacaaga tatattcctc tgacggcgac
     8641 cttgtgtgca taaacgccca tttttataag gcaaaggcta tacacaccga tgtgaacggt
     8701 gagctttcaa tggtctattt caatgacgtt accgattatg tggagcttga atatgagttc
     8761 agaatgtccc acaaagcggt aataatcata actatagata actttgacga gcttatgtca
     8821 aatatcagag agagtgaaaa ggctcacgtt gttgttgaga tagaaaagct catagaggaa
     8881 tttttggaga ataccactgc ggtatcaaaa aaggtagcaa gcgacaagtt ctatgtttat
     8941 atggaggaac gccaccttgc acctataatc gagcagagat ttaagatact cgagcaggca
     9001 agaaaaatag ctgttggcga gcgttctaac cttacattgt ccataggtgt gggcagagat
     9061 ggtgcaaacc ttgcggagag cgagaagttt gcaaagcagg ctctcgaaat gtgtcttgga
     9121 cgtggcggag atcaggcggc tgtcaaggag gacggtgagt tcaagttctt cggcggcatt
     9181 tcttctgcac ctgacaagaa caataagacc cgtatccgta tggtggcaaa ggctatgctg
     9241 gagcttatct gcaaccacga taaggtgctc ataatgggtc accgatttgg cgatcttgac
     9301 agcgtaggct ctgcaacagg cttctgctgt gctatcagaa acatggtaaa agaagcttac
     9361 gttgtggtag accctgagca gaatttgtcg aaaacgctta taaattacat aaacgaaaac
     9421 gagtcagagc attattacat aacccctcag gagggtcttg aaagacttga cgataacacg
     9481 cttcttgtgg taactgatac ccataaccct gcactggtgg agtcaaagga gatacttgca
     9541 agggcgaaaa atgtggtagt tatcgaccac cacagaagaa tggtgaacgg tatcgagccg
     9601 actatcatgt cattcttaga gccgaatgcg tcatcagcct gcgagcttgt gaccgctctt
     9661 atcgagtatt tcggcgagga ttccaatatc acagtccatg cggcggaagc tctgcttgcg
     9721 ggaataatgc tggatacaaa gaatttcatt atgaaaacag gtgtaagcac ttttgaagcg
     9781 gcagcatttt tgaagaaaaa gggcgctgat accatagctg tcaaaaagct ttttgcaaac
     9841 tctatcgaga cttatcatca aaaatcgtcg cttataaaca gtgctcacct ttataaagag
     9901 tgtgcagtgg cttatacaga ggaaagcttc tctgaaataa ggatagcggc ttcacaggcg
     9961 gcagacgagc tgcttggtat aacaggcgta aaagcttcgt tcgttatcta tgaaacgaac
    10021 ggtgtgataa atatctctgc acgaagcatg ggtgcttgta atgtgcagat cgtcatggag
    10081 tctctgggtg gcggcggtca tcttactatg gcggcaactc agctcaactg ttcaatgaat
    10141 gaggcgagaa aacgccttgc agacgccatt gacgaatact gggaaaataa ttcgcaggaa
    10201 taaaaaaaga aaggcagagt gaataatatg aaagttattt tgttaaaaga tgtaaagggt
    10261 tcaggcaagc agggagatat catcaacgtt gcagacggat atgcaagaaa ttttcttatc
    10321 ggaaagggtc ttgcgcttga agctacagct aaaaacctta atgaccttgc aggcaaaaag
    10381 gcttccgctc agcacaagat agatgttgaa acagccgaca ataaagctat cgcagagaaa
    10441 ataaaggata aggaagttct ggtaaaggct aaggctggtc agggtggaaa gctctttggc
    10501 gcagttacag caaacgttat ttcagattgt ctcaaggctc agtatggcgt tgttatcgag
    10561 aagaagaaga taaatcttac agccgagata aaggctttcg gtgactatac tgcacagata
    10621 aagcttactc atggcgtatc atgcagcgtt aaagtaaagg tcgttgagga gtaatcatgg
    10681 ctgataattt ccgtgggctt accacaggag cgacccttgg cagggagctg ccattttctc
    10741 aggaggctga ggaagccgtt ctgggaggag ttcttcgtga tccttcggtg cttccgaagg
    10801 tcattgagat actaaagcct gaaagctttt atcgtccaca gcaccagcag cttttcacca
    10861 taatagtgag aatgttcacc acaggcagca aagaggatct tatcacggta attaatgaag
    10921 ccgtcacaat gggtgttttt gaaacgtctg caatggctaa gaattatctt cttggtatta
    10981 tggaaatggt gccgtcaacg gataatatcg agagctattg caagataatc gaggaaaaag
    11041 caaggataag gtcgcttata aatgccgcta atcagatcct tgaaaaggca tatgacggcg
    11101 gtgaggattc tcagacgcag cttgacgctg ccgagcagtc tatatttgag ataagacagg
    11161 gcagagatgt tcagggcctt acacgaataa gcgacgttat cgttgataca ttcgagcatt
    11221 tgcaggagat atcaggtcct gacgcaggtg aacatcttgg cgcaaagaca ggctttggtc
    11281 agcttgacgc aatcacaaca ggtcttaaca gaactgacct tatcattatc gctgcacgtc
    11341 ctgcaatggg aaaatctgca tttgcactta atattgcggt gaatacctgc aaggcgacaa
    11401 agcgtgacgt tgttatattc tctctcgaaa tgggtaagga acagctcgtg tcccgtatgc
    11461 tcgcttcaga ggcacttgtt aataataccc tgcttaggtc gggcaatctt gagccttccg
    11521 attgggagaa gatagcaggt gcggcagata cgctgtcaca gcttcctata tattttgatg
    11581 acggtgcagg ctgtacagtg ccgcagatga aagcaaagct cagaagaatg agaaatcttg
    11641 gacttgtggt catcgactac atacagctca tgcaatcagc aaataaaaac gccagccgtg
    11701 ttgaggcggt gtctgagata acacgttcct tgaagcttat ggctaaggag cttaatgtgc
    11761 ctgttatcgc actttcacag ctttcccgta gctcagagaa gcgtgacgat aaaagaccta
    11821 tcctttcaga cctgcgagaa tcaggttcta tcgagcagga cgctgatatc atcatgttcc
    11881 tctatcgtga cgcttactac agcgatgagg gcgacaagtc ggtggctgag tgtatcgttg
    11941 cgaaaaaccg tcacggacag acagataagg tgcagcttgg ctggataggc gaatataccc
    12001 tgttcagagg tcttgatttc aggagagatg atgaataatt ttcttgtaaa ggtagcttct
    12061 accatagaga aatttaatat ggcaaataag agcgaacgct tgcttgtgtg cctctcagga
    12121 ggtgccgaca gcgttacgct ccttttgtgt ttatatgagc tgggctacga tgtatgcgct
    12181 tgtcatgtca atcaccacct ccgtggggag gaaagcgaca gagatcagca tttttgtgaa
    12241 aagctttgcg agaggcttgg ggtcgagctg aaggtgtttc atgccgacgt tgtgggctac
    12301 tgcaaggaac attctgtatc cactgaggag ggggcaagaa agcttagata tgactttttc
    12361 gacagtatcg gcgcagacaa gatatgcaca gcacacagtc tttcggattg ccttgaaact
    12421 gccatattca acctcgcaag aggtacaggt ctgaaaggta tttgcggaat accgcctgtg
    12481 agggaaaata tcataagacc gcttataaat tgtactcgtc aggaaataga gggctttctc
    12541 aaagagagag gacaggactt tgttactgac tccacaaatc ttactgacga ttataccaga
    12601 aataagattc gccaccttgt tgtgccaagg cttgcggagc ttaacagttc gctttttggc
    12661 tcatattcca tgacagctga caggcttaga acagacagcg actatcttga aagtcagggg
    12721 cttgaagcct ttgagaaagc ggtcttgcca aagggttatg acgcattgtg tctaagacag
    12781 cttcacgaaa gtgtacgccg tcgtgcaata atgcacattc ttgagaaaga ggggcttact
    12841 gtgagccatg acagtatcga ggatatcgac aggctgatac ttgacgttgg caaggcgaac
    12901 atcaagggcg gagtctttgc ggtctgctca aaaggtgtgc tcagctttgc aaagggcgag
    12961 atgcttgaaa acagcactct caggagcgtt aaggtgagcg acgagggcgt ttatcagttc
    13021 ggcaataagg agatagagtt caaattctat ccttttgacg gaaaaatcgc aaatgttcat
    13081 aaaaagtttg caaactgttg cctggattat gataaaataa agggtgagat tgttgtcgaa
    13141 aatcgcagag agggcgagaa gatacgccta gtgaacagag attttgacag cgatgtgcga
    13201 aagctggtga acaagtcctt cagacttgag gacaggagca gtgccgttat cctaaaagat
    13261 agtcagggcg ttgttttcgt tgaaggaagc ggtgcggctg aaagggtgaa gatagactct
    13321 gaaaccgtta aaatattggc ttttaccata aagtgatatg aatatcaccc taagctgaaa
    13381 agttttttgt gtgaacttgt attttttcac ttttctgtca cattgataat atataatcag
    13441 agaggttgtt agttaatgaa caatatatcg gttctttatt ctgaggaaga gcttgcattg
    13501 tctgtgaaaa agcttggagc agagataact gaagagtata aaggaaaaaa gctccttgtt
    13561 gtgtgcgttc ttaagggagc gttcgtgttt actgctgatc ttgtaagaag tatcgacaac
    13621 gatatgcaga tcgcttttat tactgcaaag agttatgttg gcacaagctc aacaggaaat
    13681 gtttcaattt caggagatca tgattttatg ggtcttgatc tgagagagtg ggacgtttta
    13741 ttggttgagg atatcctcga cacaggaaga acgctttgcg agttaaaaaa gaagtttgcg
    13801 tcaatgggtg cgccaagtgt taagatagct gttatgcttg ataagccgtc aagacgtgtt
    13861 gcagacataa cacctgattt caaatgctat gagatcgagg acaaattcgt tgttggctgt
    13921 ggtcttgatt atgatgagag atatagaaat cttcctttta tcggtatagt ttccgaataa
    13981 ggagccaaat ataatacaga acaaaaggtt gtgatcgatt gaaaaacaat aataatggaa
    14041 agaccctgtt gatttatctg ctggtatcgg ttgccataat ctgcggattg gtctatatgc
    14101 taacaagcat gagcacaaaa tcttccgaca aaaaatattc ggagataatg gaacagtttg
    14161 acagcctgaa cgtgtcacag tttgagcttg atctcggctc cggacagctt aagtataagc
    14221 tcaagggcga ggataaggta tatagttata cagttccaaa tgtttcactg tttgcaaatg
    14281 aagtccttgg cggtgaggac gctgagaact atcgtaagaa atataatact gaaaatccag
    14341 acgatccttt gcagtataat cttataccga tatcggataa cagcttctgg ctcaatctga
    14401 taccgacgct gcttatgctg ggcgttatga tattcttctt tgtgttcatg atgaagaatg
    14461 caggcggcgg aaagatgtca tctttcggaa aaacaaatgc caaaatggcg ccaagcagca
    14521 agaaagctac ctttgatgat gttgcaggtg cagacgagga aaaggaagaa cttaaggaga
    14581 tcgttgattt tctccgtgat aataagaagt ataccgagat cggtgcgaga atacctaagg
    14641 gcgttctgct cctcggccct ccgggtacag gtaaaacgct tctcgcaaga gccgttgcag
    14701 gcgaagcaaa agttccattc ttctcgatct caggttctga ctttgttgag atgtttgtgg
    14761 gcgtcggtgc ttcaagagta agagatcttt ttgagcaggt taagaaaaac gctcctgcta
    14821 tcatctttat agatgagatc gacgcagtgg gcagacagag aggcgcaggc cttggcggcg
    14881 gtcatgacga gcgtgagcag acacttaacc agcttcttgt tgaaatggac ggctttgagg
    14941 ataacgattc tgttattgtt atggctgcaa caaacagacg tgacatactt gaccctgcac
    15001 ttcttcgtcc gggcagattt gaccgacaga tacttgtggg ctatcctgac gttaagggca
    15061 gagaagctat actgaaagtt cacacaagaa ataagccgct cgctcctgac gttgaccttg
    15121 aaactattgc aaaatcaact gtaggcttca caggtgcaga ccttgaaaac cttgtgaacg
    15181 aggcttcgct ccttgcggca agaaagaata aaaaggctat cacaaaagat gagcttgagg
    15241 aagcttctat caaggttgtg gcaggtcctg agaaaaagtc taaggtcatc acagaggacg
    15301 aaaagaagct taccgcttat cacgagggcg gtcatgcact ctgcacatac tacagcaaat
    15361 cacaggataa agttcatcag gtatctatca tacctagagg tcaggctggc ggtttcacaa
    15421 tgagcctgcc tgtcaaggat aaatcttatg ttagcaagaa cgagatgtac gaaaatatcg
    15481 tggttcttct tggcggacgt gttgctgaaa agctgatcct tgacgacatc tcaacaggcg
    15541 cttcaaatga ccttgagaga gctacttcca cagctagaaa catggtaaca cgctacggct
    15601 tcagtgacaa tcttggtcca gttgtatacg gtcagggcga tcatnnnnnn nnnntatttc
    15661 ttggcagaga ttacactaat acaccaagct attccgataa cgttgcggct gagatcgata
    15721 atgagataag aactcttatt gaaagtgcat ttactgacgc tgaaaagata ctcaatgagc
    15781 atatggataa gcttcatgtt gtggctaagt atcttatgaa gtatgaaaag gttgacggtg
    15841 caacatttga gaagcttatg aatggcgagc ttacagaaag cgagtttatg ggcgaacctg
    15901 ataaaacacc agaaatccag gatactcctg agatagttga tgatatttca gttgacgata
    15961 attgatatgg aagatcagac gtgtaaaagc gtctgatttt tttgtgatgt ctgcttgatt
    16021 ttgcagtaag gatagtgtat aataagggta actgtaaaat gcagtataga acggagatag
    16081 caaatgaact tactttttat cggagatgtt gtgggacaaa gcggctgcga ttttcttgaa
    16141 agcagaatgt ataagctgaa gcgtgaatat gatatagatg tgaccgtggt gaacggtgaa
    16201 aattccgctc agggcaatgg tatcacaccg cagtcttaca gacagcttct caacatgggg
    16261 gctgacgttg taacgacagg aaatcattct ttcagacgac gtgaggctga ggagctttat
    16321 gacacaaacg agcaactttt aagacctgcc aattaccctg agggcgttat gggtcatgga
    16381 gtgacttata tcgatatgtg tccttataag atagccgtgg taaatcttat gggaacgatg
    16441 tatatggaag cagtggaaaa tcctttcaac tatgttgata agcttcttga aagcatcgat
    16501 acgcctaata taatcgttga ttttcatgcg gaggcaactt ctgagaaaaa ggctatgggc
    16561 ttttatctgc aaaaacgatg caccgctgtg cttggcactc atacccatgt tcagacggct
    16621 gatgaaacga tcttaggcgg acataccgcc tatatcaccg atgttggaat gacaggccct
    16681 gagctttccg tacttggggt ggacagtgat atagtcataa ataagttcaa atatcgcacc
    16741 cctgtgaggt tttcagaaag caccaatgat tgctttataa acggagttgt ggtaaaattt
    16801 gatgaaaaat cgggaaaagc aacaaatata cagcgtgtta ttaagcgata ataacgaaaa
    16861 gtattaataa agcttgaaat tttattatgt atgtagtata atatagtgtg tataataagt
    16921 ggggtaaatt ctttgaattt acttatggag gtattcaagt ggaaattcta aaggtttctt
    16981 caaggtctgt ccctaattcg gtggcaggag cagtttcggg cgttatccgt gacagcggtg
    17041 cagttgaggt acaggcagtg ggcgcaggcg cagccaatca ggcaatcaag gctatagcga
    17101 tcgcaagggg ctatcttgca ccattgggca ttgatcttat ttgtatccct gcatttgcaa
    17161 gcgttttgat cgacggcgag gaaagaactg cgatcaagct tatttgtgaa gaaagataat
    17221 aatgcggcag caaggcgtgc taaagtcttg ctgctttttg ctacaaagga ggggacagca
    17281 tggaaactgt tttgcaaact tatgggctta caaagcagta tggaagcttt acagcgctga
    17341 gcaatcttga ccttgcgctg gagaggggta aaattatcgg tctgctgggg ccgaacggaa
    17401 gcggaaaaac aacatttatc aagctttgct gtggactcat aaaacccacg gcaggggata
    17461 tactcatatg cggaatgcct attggcgtgg aaactaaaaa agtggtttcc tatctgcctg
    17521 acagaaattt catacctgac gatatgaaag taaatgagat gcttaggttt tttcaggatt
    17581 tttacagtaa tttcaatgta gcaaaggctc aggatatgat gaacaggctg gggataaacc
    17641 ctgcggcaaa gttcaagaca atgtcaaagg gtacaaaaga aaaggtgcag ctttgtcttg
    17701 ttatgagccg tgaggctgac gtttatcttc ttgatgagcc aattgcaggc gttgatcctg
    17761 cggcgagaga ttatatcctc aacaccataa tctcaaatta caacgagcgg gcggctgtgg
    17821 taatttccac ccaccttatt gcagatatcg aacctgttct tgacgatgta gtaatgatac
    17881 aaaatggtat gctaaggctg cataaatctg ttgacgctat ccataacgaa actggcaagt
    17941 ctgtggatca gcttttcagg gaggtgtaca gatgttaggc aagcttttga aatatgagct
    18001 taaacgctcg gcaagaaagt ttttcccact tgtggtggga tatatgatag ttgcactgat
    18061 attttctctt atgctccgtt acggtgagag cgttaaaagc cctaattttc tgatgatatt
    18121 cattgttgtg agcattgcct atggtattgc cgtgggggct ttgttcactg tgggttttac
    18181 catatcgttg acgaactttc acaagaccct tttcactgac gagggctatc ttatgctgac
    18241 aatacccgtc aagccatatt atcatatttt cacaaagttc atctcttctg ttatatggtc
    18301 ggcggcaagc atgatcgttt ttgtgctttc gctgttgctt atcgacccaa cagagtatct
    18361 tgactctgtg ggagatttta taaacgcttt gggcaacggc tttgctgaag aaccgctcac
    18421 ttcgtttctg atatgcctgt atactctgac gctgtttgca ggcttgcaga tattcttata
    18481 ttttgttatc tccctttcaa actgtttcaa gcacaaagtt cttgtaggct ttgccataat
    18541 cttcgcttgc aatatgttat cctcgatgat aagcaatctg gcaggcgacc tttctctgtg
    18601 gaatatgctc tttatgccaa cgtcacgctt gcccgataca gaagtggcat tcaacctaag
    18661 acttgtgttt atgacaatgt atcagttcgc atatatcttg gcatatttcc tgttgactaa
    18721 ttttataata acacgcaaac ataatttgca gtaaaaaaag gcagacgatg agtctgcctt
    18781 ttttgtgttg tgatctttag ttaagtatcc aaatctgagc tgagttttca ggctcaaggt
    18841 cagcgaagga gagctttccg ccctttgagt ttgcaagcac ctttacatag tctgtggact
    18901 cagtggatat tctgaaacgt ctttttccca gcgcagtgac cctgaactga acaggcttgc
    18961 cgtctatttt cagatagtca tagcccttgc atgaagcgtt tacatatccg ccgtcgccta
    19021 tctttaaacc gaatgtgccg tcctctgtaa gacagaccgt cagcttctgt tcaagctcct
    19081 tgtcaggctt ttcaagctgc aatcccttag gagtaacagt gagtacaaga cctgatatct
    19141 cgtttttgaa tgtgtaagcc ttgccgtcaa gtatgtaatt gccagcgtca gcggtgcagt
    19201 catcatagaa tttcagaaga agcgctgtga cattctgctg tgtgaccggc agagtgttct
    19261 ttgcaacgtc atcaaaagct cttgggtcaa ggtgagaaat ggtgtcaacg aacttgcctg
    19321 cgtattttac aagcttgttt gaagcctcgc caaagttgtc cttgccgtag tatttcagca
    19381 gacgcttgtc gaagctgtcg gctgtgtgtc ttgtgcaggt ggtggaaacg tgcttctcaa
    19441 aagcgtaatg cacgttgttt gccttgtcct gatatttcat gtttgcaacg tgaac
//
LOCUS       DAWEMR010000024        19410 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1177_length_19410_cov_11.255386,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000024 DAWEMR010000000
VERSION     DAWEMR010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19410)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19410)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19410
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1177_length_19410_cov_11.255386"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15577..15586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5650 a         4402 c         3632 g         5716 t
ORIGIN      
        1 catactatgt aaatcaagta aagatggagg atctcaccct tgttgacgat gatattttca
       61 agatagccgt ttttgaagaa agcggcattg aaaatgacgg ctacagcaag cttaaagaac
      121 gcttcggcga aaatttcaac gtgcagcttt caggtaaata ctggacggat ataatgaata
      181 agggcgtaac aaaaggcact gcactgcgaa ccatagaaaa ccgcattgga gtacgatatg
      241 aagaaaccat ggctttcggc gattatctta acgatgttga tatgctcaac aattcttact
      301 acagctttgc aatgtcaaat gctcatgagt ctattaaaaa ggtagcaaat ttcatgacag
      361 gctcaaacaa tgacaacagc gttatgaaag aaatcgaaaa acactgcctt gcataaataa
      421 aacataaaca aagcgatctg accaaaaatc agaccgcttt ttcttataat ctcattttaa
      481 aatcatcata gccgaactct tttataagct ccaactcgcc gtcaagcttt tgaagatata
      541 ttgacggcaa aggcattccg ttgaaagtgt tgtttttaca catggaatat atagccatat
      601 cgcagaaaac aagcctgtca ccctctttaa gcggcttatc aaaggaataa tccccgatta
      661 tgtcacctgc aagacaggtc ggaccgccaa gacgataggt gtaaggcttt tcgtctgcct
      721 gccctgaacc gatgatattc ggtcggtaag gcatttccag aacgtcaggc atatgacagg
      781 ctgctgatgc gtcaaggata gcaagatcca tgccgttttt agccgtgtct atcacctctg
      841 aaacaagata tcctgcatta agtgccacag cttcaccagg ctcaagataa acctgaacac
      901 catatttgtc atgaagataa ttcacacact cgataagggt atctaggtca tagtcatcac
      961 gggttatatg atgtccacca ccaagattta tccatttcat attgtaaaga tacttgccga
     1021 acttctcctc aatatgtggt atcgttcttt ttagcacatc agagccttgc tcacacatag
     1081 tatgaaaatg cagaccatct atgccatcaa taagactgtc atcaaaatta tctaaagttg
     1141 ttccgagcct tgaatttttg aagcatggat tgtagatatc cgtttcgatc tccgaatact
     1201 caggattgca ccttattccg caggatatct tcctgccgct tttttcaaca atgcccttga
     1261 atttctgcca ctgttttggt gagttgaaca cgatatgatc gcatatctga acaagctcat
     1321 tcatgtcctt ttctgtgtat gcaggagaat aaacatgaac ttctccgtca aactcttccc
     1381 tgccaagctt tgcctcataa agaccgcttg cggtagtgcc tttcaagtgc tgtgatatca
     1441 tagggtatgt gctatacatg gaaaaagctt tctgtgccaa aagtatttta caacccgttt
     1501 tctccatgac attatccaag ataatcagat tttttagaag ctttgcttcg tcaacaacat
     1561 aacaaggtgt tttcacctta gaaatatcaa acattatttt cctccacagc tcatctatat
     1621 gaaccatcgt tgataaagtt tactgtaata tttttgtttt tcattcttgc gtccataaaa
     1681 acactaagaa cacccttggc aaattcaaga ctctcctcag gaatatcgtc aaagtcatgt
     1741 atcattactt caagcggttc ttcgacttca cagagcttct cataaagctt gtcgccgtta
     1801 gctatagaat ccatatcatg agcaagtgcc ttgctgacat atgggtaaaa catatcagca
     1861 tttaccattt taacatcgtc tatcatcaat atcatttact cgtcccccat aaattttact
     1921 gctgttccat atgcaatgac ctcagccgcg cctgccgcaa tagcagatga agcatatctt
     1981 atattaacta ctgcatcagc acccaattgt tcagcttcaa ccaccattct cttcgtggca
     2041 agagctcttg catcattcat catctcatta taggctgtaa gttctccgcc aacaagattt
     2101 ttaaagctct gcatgatatc ctttccgaca tttttggtct ggatagttga gcctttgaca
     2161 agcccaagca tttcaagctt tttgccagaa atataatcag tatttactaa gatcatcatc
     2221 ttcgcctttc tttatttctt ttatacgctg aataagcacc gttatcgcaa cgcctgacag
     2281 caatgcagga actaccagag caattatctt tgcaagcata ggaacaccat tcatatatgc
     2341 aaaaataacg ccaatagcta tataataaat agatatcaaa gcaactatca caactggtgc
     2401 tatcattttt tttgttttgg ttttcacttt tcgtcacctt taatcaaaaa ccattttctt
     2461 ttttccgttt ttgtttcgtg gatactctct tatcctgatg atattggctg tattcacgat
     2521 actttcgttt ctgtcatcac cctgagtaac tcttacccag ccatcgccta tctcttcaag
     2581 tgtgcagtct ataactgatg aaagcgagcc tccctgcgaa actgtataaa caagcacctc
     2641 ttgtccgagg tatctcgcaa aagcttcttc gatcttcatt ttttgttctc ctattagtca
     2701 acaagaacag gatcaaagtt ctctgtccaa ggcagacccc acttgttaag tgcgtccatg
     2761 aatggatccg gatcaaactc ctctatattg tgtacgccag gctttttcca cttgcctgtg
     2821 agtatcatca ttgcgcctat cattgcaggc acgcctgttg tgtaggagat agcctgtgaa
     2881 ccgacttcct tatagcactc ctgatgatcg catacgttgt aaacgtaata tgtcttgtcc
     2941 ttgccgtcct ttttgccctg gaagatacaa ccgatatttg tcttaccctt tgttcttgga
     3001 ccgagtgaag ctggatcagg aagaactgct ttaaggaact gtagcggtac tatcttcttg
     3061 ccctcaaagt caataggctc gatagatgtc atgcccacgt cctcaaggca cttaaggtgt
     3121 gtaagatagc tttgaccaaa tgtcataaag aaacgaatcc tcttgatacc cttgatgttg
     3181 agagccagtg attcaagctc ttcgtggtgg agaagataca tatccttttc accaacctga
     3241 tcgaagttat aaactctctt tatctccatt ggctcagttt ctacccactc gccgtcctga
     3301 atataagagc cctttgcgga aacttctctt atgtttatct caggattgaa gttcgttgcg
     3361 aatggatagc catgatcgcc tccgttgcag tcgaggatat ctatatagtt tatttcatca
     3421 aaataatgct tctgagcata tgcacagaaa acacctgtta cacctgggtc aaagccagat
     3481 ccgagaagcg ctgtgatacc agccttttca aatctctctc tataagccca ctgccactta
     3541 tattcaaact ttgcagtgtc ctctggctca tagtttgctg tgtcaacata atctacttta
     3601 cactcaaggc aagcgtccat gatatggaga tcctgataag gcagagccac gttgatacaa
     3661 atatcaggct tatattcttt cataagagcc acaagctcag gcacgttgtc tgcgttcacc
     3721 tgtgcagttg tgatatttgt cttggtctta tcctgaagtt cctccttgaa tttatcgcac
     3781 tttgactttg ttctgctggc gatacagata tcagtaaata cctcgctgtt ctgacagcac
     3841 ttgtgtataa caacacctgc aactccgcct gcaccgatta tcaatgctct tgacatatta
     3901 gatcattcct ttctaaattt attatcaaca gcaatcccaa ttatagataa catcatcaaa
     3961 atcgagattt ttaagctttt gtctgtttat aaaaaatccg cagatacctg catctcccca
     4021 cattatcttc tcatcgccat tgccgaattc gctgtcaagc tgcaaaagca aaaagtcata
     4081 cttggtatct tccttacgag ggtcccactg cgtaaaagca ggatagccgc ctatcttatg
     4141 accataagcg ttctgatagt tctgctcata agcctctatc tcgtcagcag gagcgatatc
     4201 catgtccata agagaattta ttttcttttt cgattgagca gcattatagt actcacaaaa
     4261 catcttctca aaccttacgt cagattcgct cataacatca gtacttgaag tgaatttcaa
     4321 gccaaactcg ccatttacag gcatactgta ctctccgtca agtccgtttt ccttgaactt
     4381 agctttgact tcttcctcag taacggtatt gtcgatatcc ttgtgataaa ctattctgaa
     4441 cgtatcttga caagtaatat catcaaaaga cattccgtaa acatcatcat ttaatatcca
     4501 aaactgcaaa agtccacttt taggaaaatc ttcaatatca gtcacctgcg aacaaactat
     4561 ctgagccagc agccttaact gcctgccctt tttgtcctct gggatagtgc cgtcatgtgg
     4621 aacatagcca attccaccga ccttgctctc aaaaatagag ggcttattac ctgtcagttc
     4681 tagtttcacg caaggtgtca gcgtcttttt ctcgacagct ttaagtgcct gctcggcaac
     4741 tttaagttct gtttcgtggt ctaaagtttt ctccgctgat tcattttgca cattaacgtc
     4801 agtattcttt ttcttaaaaa tatcaaataa ccccataatt ttcccctttt atttataaaa
     4861 atacaaaagt atctctctca gcagcttgca ggcaagtgcc gttgaacgtc ctgtctgatc
     4921 gtatacaggg gacagctcat tcacatcaaa gcccacgata ttgagctttg cgacttcctc
     4981 cactgcaaac ataagctcct tgaaagtaac tccccctgcc tcaggagtac ccgttcccgg
     5041 aaactctgaa gggtcgagca cgtcaaggtc aagggtaaag taaacaggtt tgccctgaag
     5101 ttttttcact atgctgtcaa gtctgaaaaa gccgaacttt tccatgaaag tatgctcttt
     5161 cgcaaacttg aactcgtccc tgtcaccgct tctgatgcca aattgatata tctttccgtc
     5221 gcccacaata tcccagcatc tatgaagcac acaggcgtgt gactctttca ctccaaggta
     5281 atcgtccctc agatcggcat gagcgtcaaa gtgaatgata tgcagatctg gatatttctc
     5341 tgccacagct ctgaccgcac caagagtgac aaggtgttcg ccgcctatca tgcaaggcac
     5401 tttgccgtcg tcaagtattt cagcagtgcg gtcatggata tctctaagtg ctccctcagg
     5461 attgccaaat ggcagttcaa ggtcgccgct gtcgaacaca ttcacgtcaa gaaggtcctt
     5521 atcctgatat ggtgaataag tctcaatgcc gaagctctca ttgcgcattg cagagcttgc
     5581 aaatcttgta cccggcctga acgaagttgt gctgtcatat ggtgcgccga aaaggactat
     5641 ctttgaatca tcatattcac agtcacagcc tataaaggta tgaacattct tttccatact
     5701 tctctccttt agtcacagtc ctcaagtaca tcaaggacgt aatttggcag tgcgaaacag
     5761 cccttgtgaa gctctgtgtt ataatatctt gtctttatgc cgaatttttt ccactcatca
     5821 gccttaaggt catatatagg gtgaagattt ttcgaagcaa agccgaaaag ccaatgacca
     5881 gacggatagg taggtatatg tgtctgatag accattgaaa ctggaaacgc actgcctatt
     5941 cttctgtgtg tgcgctttac catatccaca taggaatcat aataagtgct ttcatgttgg
     6001 tttatcaata ttccctgatc tgtgagcgcc tttgagcagt tgccgtaaaa ttcttttgta
     6061 aaaagtccct ctccaggtcc aaacgggtca gttgaatcaa ctataataag gtcatattca
     6121 ttttccttgc ggcgaacgaa tttcagaccg tcctcaatat agagggtaag tctagggtcg
     6181 tcaaatgcac aggctgtctg tggaagatac tccttgcaca catttactac ctgctcatct
     6241 atctccacca tatctatctt ttcaatagtg ttatatctgc aaagctcacg gatagttcca
     6301 ccgtcccccg caccaataac aagcaccttt tttatctcag gattgaccgc cataggaact
     6361 gcagttacca tttcatgata gatgaactcg tccttttcgg taagcattat aaagccgtca
     6421 agcactaata ccctgccgaa ctcatatgtg tcaaaaacgt ctatttcctg ataatagctt
     6481 tttttagaat aaagctgttt ttttacacga atggtgaaat taacatcgtc cgtatgtttc
     6541 tcagtaaacc aaagatccat aaaatgtatc tcctctctat gaatttacca ctacgtttat
     6601 gttgtttatc tcaagatcct ccgtacctgt aagaaaacag cccttttctt tacagtatgc
     6661 gatataatta agtatgtcgg aagttatctt ctcaccaggg gcaaggatag gtattcccgg
     6721 cggatagcac ataacaaatt ctccgcatat cataccgttg ctttcttcga taggcactga
     6781 ttttttctcg ctgtagaaag cttgctgcgg cggtatctca acgataggat tgatatattc
     6841 gtggtcaaac attcctgacg gatcttttga ataaagcctt ttaatttcgg aaagtgcgga
     6901 tataaaacgc tctatctcaa gctctctatc tcctgcggaa actatagcaa gtatatttcc
     6961 gatatcaccg aactctatct gaatgtcgta atcgtcacgg agaatgtcat agacctcaac
     7021 acctgccaat cccatggctc ttgtgtgaac ggaaagcttt gtgctgtcaa atgcgaagat
     7081 atcgtcaccg ttttcaagct cccgaccgaa agcgtaatag ccacctatct tatttatctc
     7141 ttctcttgca taatcggcat agtcaactgc ctttttaaaa agctccttgc cgttgagcgc
     7201 aagatttttt cttgcaagat caagggatac cataagcaga taagagccgc tcgtggtctg
     7261 agtgaggtta attatctgtc ttacataacc ctctgaaaca gtgtctgcac aaagaaggag
     7321 cgatgactgt gttaggcttc ctcctgtttt gtgtattgat actgccgcca tatccgctcc
     7381 tcccgccatt gcagagcaag gcagaccctc accaaaataa aggtgagtgc catgtgcttc
     7441 gtccgcaagc accaacatac catgctcatg ggcaagcttg acgatgcttt ttatatcaga
     7501 gcaaattccg taataagtcg ggttgttcac caaaacagcc tttgcatcag gattttcctt
     7561 gatagctttt tcaacgtcct taacggacat tccaagaggt attccaagtt gtttgtttgt
     7621 gcctggattt acataaacag gtatagcacc gcacacaaca agtgcattga tagcacttct
     7681 gtgaacgttt ctcggcatta ttatcttatc tcctgcctta cagcttgtaa atatcatcgt
     7741 ctggactgca gcagtagtac catttactat aaagaaagcg tgctttgcac caaaagcctc
     7801 agcggcaagc atttcagcat cttttatcac agaaacaggg tgacaaaggt tgtcaagtgg
     7861 cttcatggag ttcacatcgc acttcagaca atccacgccc aaaaagtcac gaagctcttt
     7921 attcattctt ccgcccttgt gaccaggtac gtcaagacgc accactctgt tcctcatatg
     7981 tttttttaaa gcctcataca caggtgcatt attctgtgtt aagttcaaat ctatcactac
     8041 cttatcagta tatgttcatg ccgctgtata tctcgatcat ctcacggcgc agactatctg
     8101 ttatgtcaag cctttgcttc ggcgtaagct catatacgtc ctgcttgaaa agataattct
     8161 gcaagtcgat atccttaatg agaagcttgg tatggaaaat gttcgactga taaacattga
     8221 catctattgc gtcatattta gtcagcgtat ttttatctat ataatcctga atcgaagtta
     8281 tcttgtgatc gataaaaaac ttttttccca aaacatctcg cgtaaagcct cttactcggt
     8341 agtcaatagt gataatatca gagtcaaagc tgctgataag ataattcagt gcgtttagcg
     8401 gagaaatagt tccgcaagta gaaacatcta tatccactcg gaatgtggaa atggagttgt
     8461 ctgggtgata ttcaggaaat gtgtgaacag ttacatggct tttatcaagg tgagcgtgta
     8521 tcatttcagg cacatcgccg cctatttttc cttgattgca cgaagggtcg atactgtcct
     8581 catcgagggc tttttcagca ataagaacgt tcacagacgc accctgcggc tcataatcct
     8641 gttttgatat attaagtatt cttgcgccta tcatctcagt aacgtcgcaa agaattttcg
     8701 taagtctttc tgaattatac tgctcgtcaa tataagcaat atattccttt tgttctcttt
     8761 cagttttggc ataacataca tcatatatgt taaagctaag ggtcttggtt agattattaa
     8821 aaccatacaa agaaagcttc tgctccaacc ctaacatcac ccgtcctttc ggataaaata
     8881 atcagcctat caagactaaa attatagact tgttttcatt ataccacatc tcgatcaaaa
     8941 agtacatagt tttacgcact tttttcatat ttttttatta agcatggcat aagtgtggca
     9001 ataaaataaa aaaggttgaa atcaagaaga aaatagataa tgttattgaa tgttgaacat
     9061 ctttacaaat atttcaatgg gcaagcactg ctcaaggata taaatttcac agttgaagac
     9121 agagaagccg ttggacttat cggcataaac ggatgcggaa agtctaccct gcttaatatc
     9181 attacaggca gagagggcta tgacaagacc cctgaaggac taggctctgt gaatatcgca
     9241 ggcaaggctt ccattggctt tttaaggcag aacagcggac ttaattctga acttaccata
     9301 ggtgaggaaa tgaagaacgc tttcgctccc ctccttgaaa cactggataa aatgaaagtc
     9361 cttgaaaaga aaatggcaga tggcggagat attgacagta taagccacga atatgccgag
     9421 ctttcttcat actttgaggc tctggacgga tataggatag atgtaaaaat aaagcaggtg
     9481 cttaacggca tgggctttgg ctcaacacct acagacagag tcatctctac cctttcaggc
     9541 ggtgaaaaaa cacgtcttgc actggctaag cttcttcttg aagagcctaa tctgcttata
     9601 cttgacgagc ctaccaacca ccttgatttt gaaacgctca tgtggttaga agactatctc
     9661 aaaggctaca agggtgcaat aatcatagtt tcacacgaca gatattttct aaacaaggtc
     9721 tgcacgagaa tatgtgagat agaacaaggc aggctcacat catatcgagg ggactattcc
     9781 tcctatcttg tgcaaaagaa aatgaactca gagcgtcagc tcaaagaata tgaagcacag
     9841 cagaaggaga tcgcaaaact ggaggactat gtggcgaaaa atctcgttag ggcttcaacc
     9901 tcaaaaatgg cgaagtcaag acagcatatg cttgaccgta tcgagcgtat cgacaagccg
     9961 cttatgtact caaagccgcc gaagataaag cttgaatatg atattgagcc aacaaaggat
    10021 attgtgcgag ttgttgactg tccgctggtc gttggcgagg gtgctgacaa aaaggagctc
    10081 ataaagtcac ttaccatgaa cgttcgccgt ggagagcacg ttgctataat cggtgcgaac
    10141 ggtatcggaa aaacttccat actgaaactt atccaaggca ttatcccaca cgagggtggg
    10201 aatataagct ggggcggaaa cgtgaagata tcctattttg aacaggaaca cgctatactt
    10261 gatccccata agacagtgct tgaagaaata atggatcgct atccaagatt atcagaacaa
    10321 caggcaagaa gcgtactagg tgcagttctg cttacaggcg agaatgtgtt caagcctatt
    10381 tctgttctca gcggtggcga aagggcaaag ctttgctttg ccataatggc tctcaatcgt
    10441 ggaaatgtac ttgtgcttga cgagcctaca aaccaccttg acctaagcac aaaagaagtg
    10501 ctggaggacg ctttggcaga gtttggtgga acgataatcc ttgtatctca tgacagatat
    10561 ctactcaaca aggtagcaag caggataata gagataaagc atgatgaggt gaactcatac
    10621 gagggcaact ttgacgcata cagcgaagcg gtgaacgctg cccgtcagct caagatgcag
    10681 agtgaagcag agataaagcg tgctgaagaa gaaaaagcgt acaaagaaaa caaagcaaga
    10741 caatatcgca gtaaggaaca gcgtgctgct gacgctcaaa aacgcaaccg cataagagaa
    10801 cttgaaaagg agatcgaaga tacagaagtt ctgatatttg aacttgaaaa tgccatatct
    10861 gaccctgaga tagcttccga ctacagcaaa atgagcgaga aatgcaaaga gcttgaagaa
    10921 gccaaaaccg cccttgacca gaaaatggac gaatgggcag aactttcaga tcagctttca
    10981 taaacaaatt ttcacagaaa tcacccctta tgagtataat gatacagcac agactgacat
    11041 tatacataag gggtgtttgt tttggagata aaaaggctgt cagagtatga aataaaagtt
    11101 attcttaacg aaaaggatat gcggataatg aatatatcct ttgaagattt tgagaaaaac
    11161 aacaaaagaa gcaaagattt tttatctgaa ctcatgttta tactcaagga gacaggactt
    11221 ataaaaatat ccgaaggaaa tattgaagtt gatgttgctt tgacaagtga agaaacaata
    11281 aacatatatt ttatactacc taaaaacgac aatcatcaca ccactgaact tgccataatc
    11341 agctacgatc caaacgagct aatatcaaaa gctactgaaa tacaacaaaa atactctgaa
    11401 aagattataa aaagcagtct atatcagctt cctagtggtt atgctctaat cttcaccata
    11461 ggatacgcac gctcaacagt tgcgaaaaaa gctttactga aaacctgtgc tactgacaac
    11521 gtgattataa acaaaataaa ggagtacagc ccattattga gcagtactcc ttttgaaaaa
    11581 ctaaactatt tctcttagaa gcttaacagc ctctggcata tcgtttgcct taaaagcata
    11641 tgttcccgtt actaaagtgt ctgcacccga ttctttaagc aaaccagcag tttcgagatt
    11701 tacgccaccg tcaacctgaa gtctgatatc cctttccgtc tggtcaatca gatatctcac
    11761 ttcgcttatt ttttcaagag tgtcaggaat aaacttttga ccgccaaaac ctgggtttac
    11821 tgtcataacg agtaccatat caacagattt aagaaatggc tggatcgcac ttacaggtgt
    11881 tgcaggctta atggagattc ctgccttgat ccccctcgaa tgtattttgt caataacagc
    11941 ttgcggatca tcagccgcct cataatgaaa agttataatg tcagctccaa gatctgcgta
    12001 gttatcaata tatctgatag gatcgcaaat catgagatga acgtctactg gaacagttat
    12061 gcttttgccc acacttttca aaagcggttc gccaaaactt atatttctca caaacattcc
    12121 gtccatgaca tcataatgaa tccagtcaac accagcgtcc tcagcacgct ttatctcgaa
    12181 cgcaaggttt gaaaagtcgc aggcaagcag cgacgcagaa acgataatat ccaattgaaa
    12241 gcctccgtta attcaatatc ttcccactta atattgtcct ataatttcag tttctattat
    12301 acaacaccac tggggcattg tcaagcttgt tcgtcaagag ttaatgtata agctggaata
    12361 aattcatcaa gaaaaacatt tgcttctgga atattcattg cgacaataga ttcaagtgta
    12421 gatttttcag ctgatgcaaa atcagtattt cccatttgtc tttcttcaag gcactttata
    12481 agtgcagaaa gcttatcagc cgctttaaca agagtccatg cttcttcttc ctgtggagtt
    12541 ttacagaaaa gcggctcaaa gtcctctttc agatcatcag gaagataaga caccagagta
    12601 tttttggctt tctgctcgat ctcatcatat gcgcctttta tctcatggct ataatatttt
    12661 ataggcgtag gcaagtcacc tgtgattatc tcggtgacat catgatacat agcaagcagc
    12721 gcacatctct caggagaaac attaccgcca aatcgcttat ttctaataag tgcaagcaca
    12781 tgtgcgataa aagcaacttc aagtgaatgc tcgctaatat tctcattgat cgtgtttctc
    12841 ataagtgccc agcgatttat atatttcata cgagatatca ctgcaaaaaa tttactttct
    12901 gacatatcaa cactcctaaa ctatacacaa aaatcgggac gatgcttttc atcgtcccga
    12961 aaaatttttg atcttatctt acaattgtaa gaagaacacc agcagcaacg gctgaaccga
    13021 taacgccggc aacattagga cccatagcgt gcatcaacag atagtttgta ggaacttcct
    13081 gctgaccgac cttctgagag atacgggctg ccattggaac ggcagataca cctgctgaac
    13141 cgataagagg attgatcttg tgacccgaaa gaacatacat aagcttacca agaagtacgc
    13201 cgcaggctgt accaattgag aatgccacaa cgccaagtgc cataacttct gcaaactgaa
    13261 tgttcatgat cttatctgcc tgtgttgttg caccaactgt tacgccaagg aagatagtga
    13321 tgatgttcat aagctcattc tgagcagtct taacaagtct gtcgcagacg ccactctctt
    13381 taagaaggtt accaaacatg agcataccaa ccagtggagc tgctgaagga accatgagtg
    13441 agataacgat agttacgagg atagggaaaa taagcttttc taccttagaa acaggtctta
    13501 gctgacccat tactactgaa cgctccttct ttgttgtaag agccttcatg ataggcggct
    13561 ggataatagg aacaagagcc atatatgtat aggctgcaag tgctatcgta ccaacgccga
    13621 tattactgtt tgaggtaaga agtcttgaag atacatagat agatgtagga ccgtctgcac
    13681 cgccgatgat agcgatagaa ccacactcag ctgcactcat tccgaagaga gctgcaccga
    13741 tgaatgtgaa gaagataccg ccctgtgcag cagcacccat aagaaagctc ttagggcttg
    13801 cgatcagcgg accaaagtcc gtcatagcac cgattccaag gaatatgagt ggtggataga
    13861 tctgaagctt aacgcccatg taaagaatat ccaacagacc gcccttttga agtatctgtc
    13921 cgtaatctat atagtggtca ttgcctgcga ccgttttgta gttccagaag tctggatgat
    13981 acatctcagc gtatggcagg tttgtaagca gcataccgaa tgagataggc aaaagcaaaa
    14041 gcggctcaaa accatttgca atagccagat acataaatac gaatgaaata agtatcatta
    14101 tgatattttt ccagccgccg tctacgagga agcccgcaaa acccgactgt gacgccaaag
    14161 acgccatggt atcaagaaat gaattaatta ttgacatcta ttatcacgtt cctttcacgt
    14221 caactgcact aaaatggtct tgtattgtct attatgcctg cggtagacca tgcaggtctt
    14281 tgaggcttgg cagttttaac gctcttaaga acgagcttct tgccctctgc actgctcata
    14341 gccgaaattg cagccatgat aacagcaact gtttcttctt caatgccgtc ttctacaaca
    14401 ggagcagcag cctttattgg ctctggctta gcagcagcct ttgcagcttc agcagctttt
    14461 tttgcctctt cagcctttct tgcagctctg ctgtttataa cgtcaaatat tttgccgtag
    14521 atcgacacaa gcagaataag cagaacaagt ccgataaata ccacaactat acccgtgata
    14581 acgacagagg caacagttcc agcaccaaga tctttttggt caggactgat cgtactcttt
    14641 gccgcaacca tctgagctgc agtgttaaga aaattgatca taaaaattct ccttactgta
    14701 agtataataa aacttaatat tattatacac caaagccaca caaatagcaa gactttttcc
    14761 cacatttttt tcacaaaatt atctccaagt caacacctat ttttgttatg atttctgata
    14821 aaagtgcata aatacgcaaa aaaacttata ttttaggcac acttgcaaat atcatattaa
    14881 gatgttataa tatctttaca taataatgtt acggagatga tttttttgtc tgacaaattt
    14941 ttgaattggc ttgaggaatt taccccacac cttatagcgg cggctgttat ttttctttta
    15001 ggaatacttc ttgacaagct tgttttgaag gtcatgcaca aaggtttgag catgcgtcga
    15061 acagatgtga ctatacataa atttctcaca tccgtggtac acactgtatt gcttattata
    15121 ataatagtaa tggcactttc tgcacttcaa gtacctatgt catcaatagt cgcaactatc
    15181 ggagcagcag gtcttgctat cggtcttgct cttcaaaaca gtctttcaaa cgtcgcaggc
    15241 ggctttatca tacttttttc aaagcctttt aaatgcggtg attatgtaaa aataggcgac
    15301 tcagagggaa ctgttgacgc tatctctata ctttacacca ccctaaagac ccttgagaat
    15361 aagatgatat ttataccaaa cggagttgtt tcacaatcga ctattacgaa tataactgct
    15421 gacgacaaac gtcgccttga actgaaattc tctatcagct acaatgacga ccacaacaag
    15481 gcaatggcta ttcttagaga aatgctcaat gatgagccaa gggtaataaa cgagcctgac
    15541 aagccgcttg tggctgtctg cgagcatggc gcaagcnnnn nnnnnncttc cctgttctta
    15601 tataaaaatc tgttcacatc aatactgtct gcaattggca gcttggtttt gtcacagtat
    15661 gttttttcaa gactttcaag cttctctgct attctaagct gaccgtcaag cgcacttttt
    15721 atacactccg caaaaggact tccgcataga tcactgtagg tctgacaaaa ctcctttgaa
    15781 tacgccttga caaaaaatct tgcttctttt attatatttc cgtcagttac cattgttatc
    15841 ctccagactt gtgagcaacc ctgaacgctg atttttcaag gccgctgtaa gcatttgcat
    15901 atcttccttt agctgcggct cttttatctc actcacataa tgctcaaacc tgtctatcat
    15961 gcagttttca gacacgataa gtctttttat cgccgcaaac tgttcctcag ttatcataaa
    16021 gaccgcctcc cgatattatt ataccgaagg cggtctgttt tattctttgt gtttttcatc
    16081 gagcaaggtg gtggcgatag ctgcacaaaa gacgtttttt gcacccatct cttttaacag
    16141 cctgctacag acattgagag ttgcacctgt tgtgtaaaca tcgtcaaaaa gcagtatatt
    16201 tttgcctttt atgtctgcat ggtcaggcag tgagaaatat atctcctctg cgtgtgtttt
    16261 acgctcggcg gctgtgagag tatgctgttc tgcggtatcc cttgtccgtc caagcagacg
    16321 atgatcttct cttatcccaa gctccatagc cgtaaagtgt gcaagctttt gtgcctgatc
    16381 atatcccctt ttacggcgtt ttgatctgtg cattggcact gatgtgatga tatccgcttg
    16441 atctgcaacg ccattttctc ttagaacatt acccagaagc ttcgcagaat attccgcaaa
    16501 attaagctgt ctgtcgtctt taagctgatg tattgctttc acgcagatat cctcatatct
    16561 cataagagta taaatgctat caaaatgtag tgaatcatga tctagacact tatccttgcc
    16621 gcacttttcg cacaatattt ttttatgaaa aggcagatta cattcacaat ttttgcacaa
    16681 aagctcgtcc cattttataa aacttccaca tacaggacaa cgattaggat aaacaaaatc
    16741 aagcagaaat ttacatatca agcgtctgct gaccactgtc atcaccctct acaagctcca
    16801 ttagcatatt tttaagcgca gtatacctca aagtgcgttt attattatct atcataaaat
    16861 caacacgctt tttcgagcca actattataa gcattctttt ggctcttgtc acagccgtat
    16921 aaagcagatt tctgtaataa agcttgtcaa atccaccgaa tacagtgaga atgacaacat
    16981 caaactcaga accttggctt ttatgcactg ttatggcata ggcaagctcc agattatcca
    17041 gcatttgctt gtcatagaca gctattctgc cctcaaaatc tatagtcaca gttttaagta
    17101 tcttgttcac tgcaattatt atgcctatat cgccgttaaa aatgcctgcg ccactttcgg
    17161 ttttgtcctc ggttatatac tttttccaaa gaatttcata atcattcttg gtctgcatga
    17221 ccttgtcacc ttttctgaaa gtataaacag gggtcttgac ctctgatttt cctgcggcag
    17281 gaggattaag ctcctgctgt agacgcttgt ttagctccac agtacccgca ggacctttac
    17341 gtgtaggcga aagcacctga atgtcgtcta tgggcgagta atcgtaggct tttggcagcc
    17401 ttgttttgca aagctccacc acaagatcct gaagttctcc atattcaagc cgttgcataa
    17461 aaaagaaatc cttgtccttg ctggctaggt cgatatgctc gccgtttact atcttatggg
    17521 cattcataac aatctcagac tcctgtgcct gtctgaagat ctcttttagt gttacaacag
    17581 acattactcc gctgtcaata atatccttta acacatttcc tgcacctact gacggaagct
    17641 gatcgctgtc ccccaccaga acgagcttgc aggtaacgct tatggctctc aaaacggctt
    17701 caaaaaggca ggaatccacc atggacatct cgtcgattat caaagcgtca caatcgagaa
    17761 gattgttttc atcatgtata aacgcaagtg tgtcaccgct tgtatgcttc acttcaagaa
    17821 gcctgtggat agtttttgcg tcaaatcccg taaggtcaga aagccttttg gcggctctgc
    17881 ctgttggcgc acatatcata acgttaagcc cttgctgctg ataaagagaa attatagcgt
    17941 tgagcgttgt ggtctttcct gtacctggtc ctcccgtcag cacaagaaaa ccctttgaca
    18001 gtgcaagatt tatggcttca cgctgtattt tttcatattg tatgccatta ttttcctctt
    18061 caagatcgat cacttcggaa aagtctatct tattgtcata agagatctgc cgcattatgg
    18121 caagtcgcct tgctatgtag tcctcagctt tgtaaaaatc tgcaagcata acaaaacgtc
    18181 tgccatgttt atcgtaatag tagaggtttt cctcctcagt ttcatcctcg attacttttt
    18241 ttataagctt ttcatcaaca tcaagataac tcaccgctgt tttcacaaga ctgtctaaag
    18301 gcaggcaggt atgaccttgg tcagcgtttt gtcttagaat atatgtaatg cctgccttta
    18361 tcctgcactc gctgtcagca gggatatcag actttgccgc aactgcgtcc gcacgggaaa
    18421 atgacagatc gacgccctga atacaaagca cataagggtt tgattttata atctgctcgg
    18481 cagtgttgcc ccaacgcttc cacgccttta tgccataaga agtttctatc tcaaatttgt
    18541 taagatatga cataagagac cttgcggcaa aagtggtttt gaactcctga gatatttttt
    18601 ccgctttttt cactgttatc ccgtctatct ccgtgagcct gtcgggagta ttttctatta
    18661 tgtcaagggt atcgtccccg aacttgttca caagcttttt tgcaagtaca ggccctacgc
    18721 ctgatatagc accgcttgca agataacgct gaatcgctgc agaggtagta ggcaaagaac
    18781 gctcaaagat atgcaccttg aactgctcgc cgaacttctg atgtgaagtg aactcccctg
    18841 ttacaacgag atcttcgccc tcctcaacgt cacccatttc gccaacaact gtgataaggt
    18901 cattgtcaca gttaagcata agcacgcaga agcctgtgtc ctcactttta aatattatgc
    18961 tgtcaacctc acccttgagg gtctttaaat cttccatatt attctccgaa tgatctattt
    19021 ttcatggcat ttcagctctc tgcgcttgat aaaatctgca agttcagtta tatcaacaca
    19081 gtgaacgatg ctataatcct gttccaaacg ctttgaaaca taatttatct cgcttttgtc
    19141 aagctgaacg aggattattt ctcttccata atattcaggc tgaaaaaact cctcccaata
    19201 acagcttttt accatatgtt caagctcatg tgtattcttt ttgcatggga taagcaaata
    19261 tcctccacgc ttgccaacat ttcgcacctt ataccatttc acccaaaacc aaataacgac
    19321 cgccagcaca agagccagaa gaagtataag attcaacaca agatcacctc cacatattat
    19381 tagtacattt tatgcggaaa gcagattttg
//
LOCUS       DAWEMR010000025        19406 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1178_length_19406_cov_10.134360,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000025 DAWEMR010000000
VERSION     DAWEMR010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19406)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19406)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19406
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1178_length_19406_cov_10.134360"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    508..517
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6946..6955
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5763 a         4061 c         4265 g         5297 t
ORIGIN      
        1 gttctcaggt atctctttag ccacatatat gaaaagggaa tccttgtcct tgaccgccct
       61 tactgctttg tagtcgatat gttcatatcc tgaaagctct ccttttagaa aaataacatg
      121 gttgttgaag ccgccttcgt tttcaaaagc ttccatatcc tttttcattt cctgaatttg
      181 cagctcacca ttgtatttca aataatattc actgaaatca tcataatttg aaaacatttt
      241 ttgatagggg aatgtcatgt tggcttcact gctgcacttg tatatcttct gctcaaaagg
      301 aacgtctgta agctcacttg ttacattctc tgtctgataa agcttgttgc ttcctgcgta
      361 atataccgtc aatccgtttt cacaaacgct gtaaaagctg tcggcagaaa gcttttttga
      421 gaaaagcttc atgctgccct cagaaaagtc ctcagattct atggtaagaa caccactgtc
      481 cccctccaaa gagctttcag catgtatnnn nnnnnnngca tactgttatc tcagcctcag
      541 tgccgctttt tacgcactca cagaactcag cccagcgcat atcactttct tccgaagagt
      601 ccatgaaaat cataccatgc tctctagccg tgtcagcggt tatctcactg ccgttccatg
      661 ctgagaaaaa tatctttgca gggctgtcaa cgacttcctc ttgttcgata aacgaggagt
      721 ttttagtatt gaccacgctc tcttcagatc gaccgcaggc tgaaaaaagg gtcaggaaca
      781 ttgcagcaga caatgcaccg cagataattt ttttatgcaa cttcctgccc cccttggttt
      841 tatactgtag gggcaacgcc tcccaaggca cgcccttaaa tctcattata tcacattttg
      901 ctgattctgt taatatgtat tttaatcaaa ttagtaaacg tctatttggc tataatgtca
      961 aaatatcggt gtaaatatgg caaatatctc aagaagaggg attgaagagt gaaatgtgac
     1021 aatattgtaa ttaaactgtc attgacattg atgataaaaa atattataat atatttatgt
     1081 acctgtgctt tgtttgcagg tgtgcaggtg tgttcctgca aaaggaggcg aaatgtgacg
     1141 gcttgtgccg tgcaatatat ggataataac aagaataacg tttcccgcaa ggcttcggga
     1201 aatacggcga gcgttgtcgc aataatagtt gtgggcatat ttgtgctatg cgtgatactt
     1261 ctgcttgcca aggggctttt tgcaagcaat agttcagaag tgccaaaggg tataaatact
     1321 gccaccataa atacaaatga gtctgtggct gagccagtga acagtaccgc ccctgcggat
     1381 acgtccgtta ctgataacag cagtgtggac gaaagcggtg cggtaacaga tgtgtccagc
     1441 gacgagtcta gccagacaga tgtgcttgaa gtaatgtatg tcacagagta tgcttatctt
     1501 catgtggcac ctgataatga cgctgagaat atcgtttgta tgtcaccggg tgttcaggta
     1561 aacgttcttg aatatgagga taacggatat gtgaagataa atttcatgaa catagacggt
     1621 ccactcacag gatatattta taaggattat cttacaacag acagctctat agcagtacag
     1681 tggtaaaaaa tacagcgccg cacaaaagaa tgtgcggcgt ttttggatat gggggctttg
     1741 aagtgccgaa atataatact tttatcgagg ataaaaacgg agagcgcata gactttatcc
     1801 ttgacgttgc caaaagaagt cgccttaata tgggtatgcg tgacggcact ttgacagtgc
     1861 gtgtgcctta cggctgttca aaacagcagc ttgaaagctt tattaataac aatctgggct
     1921 ggataaagga gcaaaaagac aaggtggcgg tgggtctgcc taaagatttc tgccatggcg
     1981 aaaggctcag acttttgggg aacgagctta ccctgaccct tgttcaggct gataagtatt
     2041 tttctcccaa gatagaggac ggcaagcttc tcattgccgt gaacaagacc agcgaccaga
     2101 cctatgtaaa agagcagacc actaagttca taaacgatct cacagtggcg gagataacaa
     2161 aaagtatgca gaaaatgtct gcctccacag ggcttgtgcc tgaaaaggtc acaataaaac
     2221 cgctgacagc aagctgggga agatgtatct caaacaggca tatttccata aactcaaagc
     2281 ttatcgtttt ccgcagagag tgcatagatt atgtctgcct ccacgagctt tgccacttgg
     2341 tttttatgga tcacagcaag gatttctggg ctttggtggg caagttctgt cccgattggc
     2401 gaggcataag agaagaaatg aaaggctgac catgcaggca taccctacaa aatatactca
     2461 tgtgctttgt aggtattaac tattgacaaa tacaagatat tgtggtatcc ttttattgtg
     2521 gcaacaatat gtataacaaa gaaaaatgga ggtaatgtaa aatggtaaat gtgaacgtaa
     2581 caggcgggga gatgtcccag gcagagataa attcatatat cgacagggcg cagaagcttt
     2641 accctgaaaa gactatccag actatcgacc taaaaataga cggcgacttt gttgacgttg
     2701 attatcactt tgcagacgtg ccttttcagc gtataagaag aataacaggc tatcttgttg
     2761 gaactctcga cagatttaac gacgcaaaga gagcggaggt ccgtgatcga gtaaagcatg
     2821 gtcttgcaag ccccagcgta tcagaacagt aaaaatgaaa cacggcatat gtcaccgaaa
     2881 aagtggcata tgccgtattt tttatggaag tgtattggtg tagcttttta gaccttgttt
     2941 ccgcaaacgc tgcaaaattt ggcgccgctc ttcagctctg agccgcagtg aggacacttt
     3001 tcaggtgcgg tctgctcgac aggctgttca gccgtttctg taggtggttc tataggctct
     3061 gtctgttgtg gtatctcaga ctcaactacg ctctgtgaca taacaggctc ggcattgtcg
     3121 ggctttctga actgtgacat atcaggctga gcaaaactct gttcttgcgg tgtagtcatt
     3181 gggataggcg caggggcatt gtcagacgtc tttgcggcaa caggtatctc aacggcgaaa
     3241 tcttcgccca taagtctgcc tgcaataaca agtccaccga taaaaagcag gaaagccgca
     3301 ataaatggca gaacatcatc agcaagtgaa agggctaccc tgaacttgtc attgtcatag
     3361 cccattgtta tagcgtttga gaagtgacct tttaccgtaa catagtatat cattatgccc
     3421 tttgttatca tcacaacaaa cgggaaaagg ataagaaagc ctgcgaatgc accatcaagc
     3481 tttttcttgc cctttgctct tgcagatgtg aaaaacgcca caaggctgaa tatggcgagt
     3541 gcgcatgaaa gaccataggt gttcacataa gcgaaaatgt cgggggtgaa caaaaagtct
     3601 atcttgtttg tgaaaacgtt gttgtaaacg agctttccca cagggtcagc aagagcgcct
     3661 gctgccgaga gaatgaatgc gaaaaaactc agcagacaca gggacattcc tgcatatgta
     3721 agcttttttg tgtagatctt ttttgccatt ttgattagtc ctttcatttg tgaagtcttt
     3781 atccgctgtc cgaaaaaata ggggcggata gaatttccgt ccctattata atactatttt
     3841 ataaaagcct tgtcaataga tttttttcgc agctttcgtc gctatttatc gtttttgcca
     3901 ctatcctcgt cctcaccgag aatatttttc atcatctcgt ttatgtgccg aagcttttcg
     3961 tcatcatcat tgtcaggtcg gtatatcctc agcggctcgt ccgttgtatc agttgtgtca
     4021 tttatcacag gggttgtggc tttctcactg ctctcggccg taaagttagc ttttgcaagc
     4081 acctttttca tgacttttcc acgcttatat tcaaacagga atataagcac cagcagggca
     4141 acaccaaagc ccgttatgat aagagatact ttccaatagt caggtgagaa cttcattgtt
     4201 atcttgtttt caccctgctc caaagggata gttataaggc tgtcaagaga tttttcagct
     4261 gtcacctttt tgccgttcac agtgatagtc cagccgtttt cgtatggtat ggtcgtgaac
     4321 agtacagagc cgtcattttc agcattaaca gtgccctcaa gataagtgtc cttaaagctt
     4381 gtgacctcaa gcacattttt ctgcatatca gcgcaggtct gtgcaaagct gtcataatca
     4441 aaggtgtaga aaagctggtc tttccagtaa gcttcattat cgtcatttgc caccgttatg
     4501 cgtatgcgaa gctcctgccc ctcatagaat ttgccaaggt tcataatgga ataattgtcg
     4561 cccacaaaga actgacctgc atattccata ggctcagagc cgtccatata atcgtcctcg
     4621 tcctgtatcc acacgttgca ggaacgctca tatttggttg gaagatacat ataaagatat
     4681 gagtccttat ccattttcac aacatagtca atgtgacatt ctgcaatgga agtgtctgta
     4741 gggaagtatt tcgtgtgtcc gtcagtgagc tgagatgtag aaacgttctc catttcgctg
     4801 tttataacag gtatctttgt gaaatattcc tttgttgtgc ctgctagtgc gttgaatacc
     4861 atgttctgat tttcaaaagg attatcttca gaaagtgaca cgtcctcaat accaggcgaa
     4921 gataccatgc caagaccaag agcgttaggg ttgttgtaga atttatatgt tgcgtcgccc
     4981 tcagtgtatt ctgttgtaag cttgtattcc tcaggaactt taactcttgt gcctacaaaa
     5041 gaggtgttgc ccgttttgtc cataagatat tttatgtcaa acagagcgtc ggtcatgaaa
     5101 gttgtgccgt catattttgt atagtgtccg ccgtaagcat agccaagctt atggagcatc
     5161 ataagggcag gtgcgttcat cgttgagctt gaatgtgaaa tgcctttgta gcctgttccg
     5221 atagggtcgt tcacggttct gtggaaagtt gcctccatgc ggtagaaaag cgacgggtca
     5281 tattccttca tcattgacac agcgtttctc gtctgcgtca tgtaaggctc ataggaagtg
     5341 tacttgctgt atgcaacgtc agtgtctatc ttgtcgatag tgtcagcgct gtttgcaaaa
     5401 agctcaaccg caagaacgcc taccattacg atacaaacag cctttgattt tggatatttc
     5461 ttgataagat agataagtgc gaaatatgcc gccagcgcta tcattgatac ccatatgcct
     5521 tgtataacgt ttgtggtgtc gcctgtttca cttgtctttg tttcaaaaag ctggaagtga
     5581 gagtagttct cacgctcaca ccagaagagg aataccataa gtccgaagaa aattccgccg
     5641 atgttctttg cagttatgcc ctcgaggttt tcaaacgccc tgaatgccat tactatcatc
     5701 aggaatgaga atatgaaaga atatctgtaa ggcagccagt tcggcacttg gaagccgtgc
     5761 attgccatat cagcaggctt tatatacatt agtattacaa gcaggaaagt gagcagacct
     5821 gtagaggtct tttctttcat tgttatcctg tcgttcataa agaacagcgg aacaagtatc
     5881 aatacagccg ttccgcagta tatcataggc ataccctcag ggtaaacagt gtcatatgac
     5941 attgggaaaa gctttgttat gaacgtaagg aaatcaaatt gtgttgcaag tgagaaatca
     6001 gggtcagtga actcaaactt gccaagcttc aaagaattgt aaacggttat aagaacgaat
     6061 gccgcacaca taagcgcaac aatcgtgccg ataccgaaag ccacacagcg tgagaagaac
     6121 ttttttggca atatcctgcc ctctcttgaa aggcagtacc aacagaagta gaaaaacgtg
     6181 aaaattccca ccatatagcc gatgtagaaa tgtgcaatga acataagcgc cagcggcact
     6241 atataaggta caagcttgcc ctcatcaacg agcctgtgaa cgccccagca tataagcggc
     6301 agatagataa gtccgtcaag ccacataggg tccataagtt gtaccaccat gtatgacata
     6361 agagcataca tggtagggaa tatgataagt gagatagtct taggcttctg cactgatatc
     6421 ttttttaaga agaacatgaa cgtcacagcc gccgtgccta tctttgcaag ctgtaaaata
     6481 aggatagagg tcgccatcat cgttctcggc agcagcacaa gtatcagcat aaaaggactt
     6541 gcaagataat atgcaaacac accgaacatt tctccgctga ggtttcgtga ccagttgtag
     6601 ataaaactgc cgttgccatg gaaagcgtca cggaactgct cgtagtagta aacatactga
     6661 ccgttcaaat caagcacaag caccgagctg tcaccgatag ggtgtatctt gaaaaacgca
     6721 taggcagaaa acattattgc cacaggcaga aagaacacca aaaaatacag ccgcttatca
     6781 tagcaccatt tttttataaa tgaaagggga gatgttttag tcttttccat tctttttctc
     6841 ctcgtcgtct tttcttatga tatcacggat tatgtatcta ggtcgctgtt ttatctcctc
     6901 gtatatcttt gaaagataaa agcctattat tccaagactg aacatnnnnn nnnnngaaca
     6961 ttattatact cgatgtgaga agctgtagga ttatgaccgt tgaaaatcct accgccgacg
     7021 caccgataag ctttttgata agggtatgaa gtcccagtat gacagagaaa ataaacgtta
     7081 taacaccgca tactgtcact atctgcaaag gcgcagatgt aaaagaggtt atgctgttta
     7141 tggcgaattt gaaaagcccc tttacagacc actttgattc gcctatctct ctgtcagcaa
     7201 cgtcaaaata tacacgctcc gtcttgtagc ctacccagct tgacattgct ctgaaaaaag
     7261 tcagtctttc aggcattgca ttcagtgaat ctaccacagc cctgtcaaga agcttgaaat
     7321 cggaagccgc ttccatatca agtccgcttg acttgtttat catcttgtaa aaccacagag
     7381 agaaaaattt gtatacaggg ttttcctttc cccgggaatt ttttctgccc tcaacaatgt
     7441 caacgtcatt gtttttccag atgttgtaca tctcgattat gacctcaggc gggaactgca
     7501 aatcacagtc cagcagtacg caagcgtcac cctttgcagt ttcaagtcct gcgaaaattg
     7561 cgctctcctt accgaaattt cttgaaaagt tcacgcccac gatatgctcg tcattgcgtg
     7621 caaggtttga gattatatcc catgttctgt cctttgagcc gtcgtttaca aaaacaagct
     7681 catagtctat accgttttcg ctgagaagct ttgaaacaac agaagcacat tttgcaacgt
     7741 tgccctcctc gttatagctt ggtattatta ttgatagcat ttttcgttct cctattcttt
     7801 gttgccttta tacacaaaac agaacgccat ttgtgaacat gacgttcttg aaatatcctt
     7861 atttgttttc tctttcacgg cgtttttcat aatctgtcct cagcttgttg aagttgtaaa
     7921 gcatgatgag caggaaaata tacacaagta tctttgctat tgccgcaata acgcctgcaa
     7981 gggtggtgac gaactctatt ggtattagcc tgttgagcag gtcgccccca aagtaaataa
     8041 acgtcatgag agcaagcttg ttgaaactgc cctgaagcct tacaatgttt gaaacgtctt
     8101 ccacaagctc tctgtggctg tcaagcgcca caaggaacat tctcataagg ttcagtgaca
     8161 tatagacagt tgccgccgac agcaaaatgc ttataacgtt cgccgccatt gccgcagggg
     8221 atagaagctt caatagaagc tgacatacca ccgcaagagc gcacatagcg gaagaagtgt
     8281 aagcaggctt tttaagatgt gcgaaagcgt cagtgcgttc ttccgcctca gccgttccca
     8341 ctgcgaaaaa taaaaagcct acaagcttta taacgtaatt agaagtccag cctgcgttta
     8401 gcatgaaaag catgaaaaac gagccgacca taaagaaatt cattgtttaa gaccctttcg
     8461 tagctgatta tacctattta ttatatctca tttatatacc cttgtcaacc gaaaacgcct
     8521 tgttttcacc ttttgtacac aaagcgtcag ccctgtattc cgtaggtgac acaccctttg
     8581 tcttgtggaa tatccttgaa aaataaaggc tgtcattata ccctaccgac cttgccacgg
     8641 ccgacacagg aagctcagtt tcttccaaca aacggcaggc ggcgttcaca cggaaatcag
     8701 ttatatactg tgcaggagaa agcccctcgt attccatgaa agctcgatat agcgtggcac
     8761 ggctcacgcc aacgaaatcg gtggcattct cgacccctat cccaagggtg tagttgtgtg
     8821 caatgtagtt tttcgccttt gtgacatatc cctttgcggt gctccccgaa cggctttgac
     8881 ttcccgagcg ttccataaga atgcccagcg tgatgtaaag ctgacctgtc attttcacaa
     8941 ggtctgagct tctgtttcca ctaagacgat aaatgcgttc aagactgtct ttaagctcgc
     9001 ttccaaagtc tgcggagata acgggacact ctcttgtgaa gcctgcacga tcaaggataa
     9061 attcagcgtc agagccgttg aagcctgccc agtagtattc ccatgggtcg ttcttgtcgg
     9121 cagtgtatgt tataacagtg taggggtaaa tgagaaaagt gtcccctgcg ctaaggtgat
     9181 actctccttg aggtgttttg tatacgccct tgccacttat gatgtggtgg ataagataat
     9241 ggtcacgaac gccgcttccc caactgtgaa gcccctgaca tttttcatag cccacgttgt
     9301 atactgacag cgaaagtgcg tccgtctgat ctgatttaaa ggaatggcgg tctgtattca
     9361 ttctgtgtga tcctttcagt gagatggtct gttatagata tgatagcata tttttattct
     9421 ctttgcaaca aaaatccatg cgtttgaaac aaaaatggta ttgcaaaaac gtgaaaatat
     9481 ggtataatgt aagtgtaaga aaaaacctga catatttggg aggtaaagaa tatggcaaaa
     9541 atactcgtta caggcggagc aggatttatc ggaagccaca cctgcgttga gcttcttgaa
     9601 gcaggctatg aagtagttat agttgataac ttttcaaact caaagccgga ggctcttaac
     9661 agaataaaga agataacagg caaggacttt gctttttatg aggcagatct tcttgacctt
     9721 gcagcgctcg aaaagatatt tgaagaaaac aagatagacg ctgttataca ttttgcaggt
     9781 cttaaagcgg ttggcgaatc tgttcagaag cctgttgaat attaccacaa caacatcaca
     9841 ggcactctca tgcttatcaa ggcaatgaga aaatacggct gtaagaagat agttttctca
     9901 tcttccgcaa cagtttacgg ccctgtaaac aaagctccat atacggagga tatgcctaca
     9961 tcagcaacaa acccttatgg ctatacaaag gtaatgatag agcagatact gagagatgtt
    10021 tatgtttccg acaacgattg gtcagtttca cttctgagat acttcaaccc tatcggcgca
    10081 cataagagcg gtcttatcgg tgaagaccct aacggtatac caaacaatct gcttccttac
    10141 atctgtcagg tagcagtagg aaagcttgaa aagctcggtg tgtttggtga cgattacgat
    10201 acccctgacg gaacaggtgt aagagattat atccacgttg ttgaccttgc taagggtcac
    10261 ctttgcgcag tcaagtatgt aatggaaaac aagggtgttg aagccgtaaa cctcggcaca
    10321 ggaaaaggct caagcgttta tgacgttctt cacagctttg aaaaagcctg tggaaagaag
    10381 ctcccatatg tagtaaagcc aagacgtgca ggcgacctcg caacctgcta tgctgataca
    10441 gcaaaggcaa agaaggtctt tggctgggaa gcacagtatg gtcttgatga aatgacagca
    10501 gatagctgga acttcattaa gaataatcct aacggacttt aatacataag catagaacat
    10561 aatagttttc ctaggcaggg acacacaaaa aagtgtgttc ctgtttttta tgcccttttt
    10621 tcattcatac aataatgcga caaaaaaatc ccactgtaag tgataatata ttttcagcgg
    10681 ataaaacgaa aagatgaaag gaatgataaa gcatgaatgt ggagataaaa aactatccgt
    10741 cagccagaac tcttgtggca gagatatgcg gcgatatcga ccaccataca gcgaaatatt
    10801 tcaggggaga gatagataaa gccattagaa gccataaccc tcttaccctt atcctcgact
    10861 attctcaggt gacttttatg gatagctcag gcatcggact tgttatgggc agatacagga
    10921 taatgtcaga aatgggtggc gaggtgatag tggcgtcgcc tcctgcttat atccgaaagg
    10981 tcttgcagct tgcaggtatg cacagactca caaagatagt tatggatatg accccgtata
    11041 tacaagagaa gaaagcagct caggaacaga atgaaagccc cgatgacaaa acaaaggaga
    11101 gtgataaaat tgaaacgaaa accccttaat gaaatgaaag tcagttttcc cagctattct
    11161 gaaaatgagc gctttgctag agttgcagtc agcggtttcc ttactcagct tgaccctcag
    11221 atcgacgagc ttgcagatgt caagaccgca gtgtcagagg gagtcacaaa cgccattgtc
    11281 cacggatacc gaaacaccct aggggagata ttcatgcaaa taaagatatt cgagaacaga
    11341 cacgtctaca taaaaataag ggataagggc tgcggtatcg ctgatatcga gcaggctatg
    11401 cagccgcttt tcactactgc acctgaagag gaaagagcag gcctaggctt tgcggtcatg
    11461 gaaagcttta tggataaagt gaaggtgcgc agcaagcttg gagaaggaac tacccttatc
    11521 cttgaaaaaa ccttgaaaga aagagtataa aatgagcagc atagtcgaga cccctcttgc
    11581 agaagaaaat ctggggcttg ttcacctgtg cgcaaaccgc ttccgcaatc gtggcataga
    11641 gtatgacgac ctttacggtg caggctgtat aggtcttgta aaagctgcga aagcctttga
    11701 cagcgaccgt ggtgtgaaat tctccaccta tgcagtgcca gtgatattag gggagataaa
    11761 aaggcttttc cgtgacggtg gcacgataaa agtgtcacgg agcataaaag agctttccat
    11821 gcgccttgta agagagcgtg agcgattttc catacagcag gggagagaac ctacagtaag
    11881 cgagctttgt gagctgtcag gagaaagcga agaagccgtt actctagcca tgaacgtcgc
    11941 cctgccccct gttagtctta ctgaaagctc agatgaagac gaccgccacg gacaaataga
    12001 tatccctgtt gatgctcctg acatagaaat tgctgacagg ctctctctcc gtcaggtcat
    12061 agcttccctt gaatcggagg atagaaagct tatatacctg agatattttc agaataaaac
    12121 tcagatggaa acggcaaaac aacttggtat gtcgcaggtg caggtgtcaa ggcgagaaaa
    12181 aaagctgatc actttaatgc gtgaacagct taaataatat cattacagtg ggcggctttt
    12241 ggtcgcccat ttttttataa ataagacctg agtatctcga tagtttcttc atgtgagcaa
    12301 acagcgtcag aatctataaa gtgctgaaaa taatcccaga aagcctgaac aaggctcgtt
    12361 tcgcttatct tcaaataaga aaaatcagag aagcagttgt ttctggcaat gatgttaaga
    12421 cttgtgttgt cagaaatgtt gatacaaaga tttttcgaca ccgcaaaata gtcgttcttt
    12481 attatcctgt agtctgccat gccgttttca caaagcgata tcattatctg cagcattatc
    12541 ttgcgttctg caagagtggg aaaagtatat gaatttctcg gaagctcaaa aatctctccc
    12601 gtcgtcataa actctgcaag acccttttca ctgaacagat ttacaaattt gtgttcagaa
    12661 agagccttct tgcccatggt gagctttgct tttacaaatt cctttgcgtc agccggcaaa
    12721 agtgcatggg cattgtatat atcctctgta agacaaagca tggtgcatgg ctgaaaatcc
    12781 acgccgatga aatttgcgtt gtcatcgtac caaaagtaaa catattccaa aatatccacg
    12841 gcgtggtcga gaagctttgc acattttgtc ttgaactttt caaactgccg catataaaat
    12901 tctaccatag gcttagatct gtatataacg ccctcacaat agtcgctgtc tgttctgatc
    12961 agaaaattat tcgttatcat tatgtttggc atgatgttcg tggagttgaa atatgccgac
    13021 gcaccctcat aataataaaa agcctcatat ttttcatgct tttgcgcaag gtgacatatg
    13081 aacggaaaaa tatccagatt gtattttgcg gtgctttttt cgcccgtggt gttgtcaaag
    13141 aagaacaaat ggtttatggc agactcgctg ccttctgcgg caatggacag cagattggta
    13201 agaaactctg ttggctgagc gatgacctga atgtcagaac cgttatgaac atcgctgcat
    13261 ataagcacct gcaggttttt tctaagctca cgtttgtcgg tgtagattga tatctcaggc
    13321 acagtgtcaa tatcaacttc tgtcttgaaa gtcagagcgt caggctcttc catgttggtg
    13381 tttgccatat cgtgtattat ctttgacacc tgcttacggc gattgtagac atcttccccc
    13441 atctcagaaa tgttataaag ctccatgagc ctgcttttat cctctgtgga aagcatgagc
    13501 tgcaagctta tattttctac aaaatctacg cttggcactt tcctttcccc tttgagtatc
    13561 ttatgaagat atgttctgtc gataccacat ttttcggcaa gtgcttttat agtaatatct
    13621 tttttcttta tgtattctct gagaagttcc gaaaattgtg acattgtcat catatccttt
    13681 ctgtagaaaa caggcattta aagtatattt aaagtatatt tttacatacc gatacctata
    13741 ttatatccta tttatacaca attgtcaacc tttgataatt ttatcttatt aaggcaaaaa
    13801 ccgtatattt ttataaacag ctgcaaaaaa tacaagcgta aaatgaaata ctttaaaatt
    13861 tggaggagtt ttcatgaaaa taatattcta tgacgcaaag cagtatgata tagattcatt
    13921 tgaaaagcag cgaaaaaact atcccgatat agaaagcgtt taccttgaag ccgacttgac
    13981 ggaagacaca gcaaggcttg ccgagggctg tgagggcgtg tgtgcctttg taagctcaac
    14041 gataacaggc tcagtgctta gacggctcca tgaacacggt gtaaagcttc ttcttatgcg
    14101 atgtgcaggc tataataatg ttgatataaa agccgccgat aaatacggca taacagtgct
    14161 tagagtgcct gggtattctc cggaagccgt ggcagaacac gccatggcgc ttgcgcttgc
    14221 agtcacaaga agaatacaca agggctatat aaaggtaaga gagaacaatt tcagcctgaa
    14281 tggtcttatg ggacttgatt tccatggcaa gaccgctggt ataataggca caggcaggat
    14341 aggtgcggca atgtgcagga tatgccacgg ctttggtatg cgtgttatag cttatgacgt
    14401 ttatcaaaac cctgaccttg acttcgtgaa atattatccc cttagcgaag tcctgagaga
    14461 aagcgacctt atctcgctcc attgcccact gacagaatca agctatcatc ttatcaatac
    14521 tgaaactatc tcccttatga aagagaatgt tgttttcgta aatacctccc gaggtgcgct
    14581 tgtgaatacc gaagacctta taaaaggtat cagaagcaga aaattccacg gagtgggtct
    14641 tgatgtttat gaggaagaaa cagagaacgt ctttgaaaac cgacaggagg atatcctaga
    14701 aagctccgtg accgcaaggc ttctgtcttt tcctaacgtt atcgtcacct ctcatcaggg
    14761 ctttttcacc gaagaagccc tagccgccat aagccgcaca acgcttgaga atgcttgtgc
    14821 ttttgagcgt ggcaggataa taaaagaaaa tcaggtgaaa tagacctacg ggaatattcc
    14881 cataagtctg gcatgatata aaaaaaacgg acagtcagtt gactgtccgc ttcgtatata
    14941 gctttgttta tacgcttata ttagtaagcg ataccctgcc acttcatagc gtcagcgacc
    15001 ttcaggaaac ctgcgatgtt agcacctgca acgaggttgc cttcaagacc gtactcctta
    15061 gctgcattgt atgagttgtg gaagatgttt gtcatgatgt tcctgagctt agcgtcaact
    15121 tcctcgaatg tccaagaaag tctctcgctg ttctgtgaca tctcaagagc tgatgtagca
    15181 acgccgcctg cgttagcagc cttagctggt ccaaagagaa cgcctgcatt ctggaataca
    15241 gcgatagcct ctggagttga aggcatgtta gcaccctcag caacagcaaa tacaccgtta
    15301 gctacgagag ccttagctga ctcttcgttg atctcgttct gtgtagcgca tgggagagcg
    15361 atgtcgcact tgatagtcca aatgccgctg cagccctcgt ggtactcagc accctgtact
    15421 ctgtttacat actccttgat tctgcctctc tcaacttcct tgatctgctt aacaacatcg
    15481 aggttgatgc cgtttggatc gtatacataa ccgttagagt cagaaagtgc aactaccttg
    15541 ccgcccagct ctgtagcctt ctctgtagcg tagatagcaa cgttaccaga accagagaca
    15601 acaactgtct tgcctgcgaa tgtttcattc ttcatgcact tgatcatctc agaagtgtag
    15661 tagcaaagac cataaccggt agcctctgtt cttgcaagtg atccgccgta tgaaagaccc
    15721 ttacctgtaa gaacgcctgt gaactcgttt ctgattctct tgtactgacc gaacatatag
    15781 ccgatctctc ttgcacctgt accgatatcg cctgctggaa cgtctgtgtc agcaccgatg
    15841 tgcttgcaaa gctctgtcat gaagctctgg cagaatctca taacttctcc gtcagacttg
    15901 cccttaggat cgaagtctga accaccctta ccgccgccga taggaagacc tgtaaggctg
    15961 ttcttgaaga tctgctcgaa gccgaggaac ttgattactg atgcacatac tgaagggtga
    16021 agtctgatac cgcccttgta tggtcctata gcagagttga actgaactct gaaacctctg
    16081 tttacctgta ccttgccgtt gtcatctacc caaggaacac ggaaaatgat ctgtctttct
    16141 ggctcaacta ttctgtcgat tatacctgcc tcaacaaggt caggtctctt ttctacaaca
    16201 ggaatgaggc tttccagtac ctcatatact gcctggagga attccttttc gccgggattt
    16261 ctcttctcaa ccttctcata aacgctttca agatacgcat tgttcaaagc cattattaaa
    16321 actcccatct tataaattat aatttgtgca cggctttttt tactcattcc gcacatcact
    16381 tacttattat agcacactat tttgcaattt gcaagtagta aaatgcaaaa tttcataatt
    16441 ttattgaaat tgtataaaaa tcatagcctt tttttgaaat gtgaagtaaa atcacgatta
    16501 taaattcata attcgtgtaa aatttccgtg aaatgtcgac aaatcattgc actgtgacca
    16561 aagctgtttc agcctgcaaa aatgacaaaa cgcattttta tgccgatata acgcacaaac
    16621 ccgctggaat atcgtccctg atatgaaatt ttggttgacg aatcctgcaa aaaagtgtac
    16681 aatataaagt aataaagcac ttataaacaa aggagcagta ccgatgacaa agaaagaacg
    16741 tgcacaaatg atagtggaaa ggctggagca ggtctacccc gagggcatat gctcccttga
    16801 ctataaaaag ccccacgagc ttatgatagc aggcagactt tctgcacagt gtacagacgc
    16861 aagggtgaat atcgtcacga aagaactttt tgcaaaatat aagaccattg aggattttgc
    16921 taacgcagat gttgctgata tcgaggagat aatacgtccc tgcggtctgt ataaaacaaa
    16981 agctcgctct atcgtagcaa tgtgcagaca gataatggat aactttggcg gtgagatacc
    17041 tgataccata gaaaagctca ctacccttgc aggcataggc agaaagacgg caaatcttgt
    17101 tatgggagat gtttttcata agcctgcggt ggtaactgat acccactgta taaggatatg
    17161 cggcagactt gggcttacaa aaaataaaga gcctgcaaag gtggaagagg atctgagaaa
    17221 gatacttccc cctgagagat cctctgattt ctgtcaccgc cttgtactgt tcggacgtga
    17281 atactgcaag gcaaggggag aacgctgtag cgagtgtccc ctgcttgata tctgcggcag
    17341 cagaaaatga ccgcaggctg ttgaaataat aaggcaaaag tagtataata aggtcacaag
    17401 atgaaatgca gaggagatga gcgttttgat aaaggcatgg gagcatttca agaccattac
    17461 aagacatcgc cacggagtga taaaaaactg ctataaggca ggcattttgt ggcagggtct
    17521 gaggcatgac ctttcaaaat attccccgga ggagtttctc aagggctgta aatattatca
    17581 aggcacacgt tcgccccatg aagcagagcg tgaagaatac ggattttctt acggctggat
    17641 gcaccacaag ggaagaaata agcatcactt tgaatattgg acggactatg acctgcggac
    17701 taagttgatg acccctgtga aaatgcccct taaatatgtg aaagaaatgt tttgcgacag
    17761 agttgccgca agcaagatat atatgaaaga caaatacgac gacggtgcac ctcttgcgta
    17821 ctttttaaga gcaaaaaaga caagagccat ccaccctgaa acatcagacc ttttggagaa
    17881 tcttcttaca atgcttcgtg ataaaggcga ggactacact ttcgcttata taagacgtct
    17941 aaaaaaatat tagggggaat ttccatggga attttcaaaa agaaaactgt caagcccatt
    18001 ccggaggagt gcaggggaat ggagataaaa ataatgtcaa gcacctgcac aggcgagaaa
    18061 actatcggat tttataaccg caacactcgt gagataatgt atcccgagct tgtgaaaagc
    18121 gacagcgatg ttgaggcttt ttatgaaaaa tacggattga aaaaatgaat tatgttgcat
    18181 gagagaatat ataaaagaat gtaaatattt aatgaacttt gagatgaaat atatgtaaga
    18241 atattttatg ggtgattttt acagaacgcg aaaaataggc gtttataaaa aaataatgat
    18301 gctgattaaa agttaattat ggctaaatta taaatactgt ttccaacgtg tgaataagta
    18361 caaaaatcaa gcggcgacta tgcctctttt ttatgtaaag ctacaaagat tgcaagaaag
    18421 cacgcaaaat cgggcaaatt aattgacaag aggggtcaaa tacgttataa taacagcata
    18481 aaaacaatgt aaaaaattat aacggaggta gattcaaaat gacaaaaaat gatctgatat
    18541 ccgcaatagc agaaaagggc ggctattcta aaaaggaagc agagcaggca cttgcggctg
    18601 taacagaaac tatcacagag gcacttgtaa agggcgaaaa ggtatctatc gtaggttttg
    18661 gtgcatttga ggtaagagat cgtgcagcta aggattctat caatcctgca acaaagaagc
    18721 ctatccatgt tcctgcaaag aaggttcctg cattcaaggc aggcaaggct cttaaagagg
    18781 ctgtggataa gtaattagta acacttttaa aggagaggtt ttacaatggc tgaaaagaag
    18841 atcgctgctg acaagaaggc agaagaagct gtaaaggaag tcaaggctga ggtaaagtct
    18901 gccgctgaaa agacaggcgc agcagtaaag gaaacagcaa agaaagctgt caagacggca
    18961 gaaaaggcag taaagaccgc agcaaaggca acaaaggcaa agaaggaaac tgtaaagaaa
    19021 gcagttgagg ctgagataaa ggacgaggta ttcgttcagt tcgcaggcgt agagataaat
    19081 gttgaagctg tagtggctgc tgcaaaggct gactacaagg caaagggtca cagaacacca
    19141 aagacagttt cagtatacat caagcctgaa gagggcgttg cttactacac tgtaaagggt
    19201 gtaggctcag aggattacaa ggtagagctc taattgtaac atttgtgtgt aggggcgaac
    19261 agcgttcgcc cgctatctaa cacgttctat ctgtatgata agctacatca agcggaggac
    19321 aacacctccg cttttttgcg tatataaaca gaaaggcgct gtaaaaaaag cgcaacaaag
    19381 cccgtggatt ccctcgatat ggaagc
//
LOCUS       DAWEMR010000026        19173 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1191_length_19173_cov_10.839366,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000026 DAWEMR010000000
VERSION     DAWEMR010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19173)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19173)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19173
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1191_length_19173_cov_10.839366"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6615..6624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16493..16502
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17617..17626
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4941 a         4572 c         3840 g         5790 t
ORIGIN      
        1 gtgcattact tccatttggt gttatggata acaggtagac atagtttccg cattttaccg
       61 aaaccgaatc agactcgtca gttaagtaat ctccggtgta cctatatctt tctaaagaag
      121 tgtcaagaat atcacacgaa tcgctaatat cttcagaaga aagaccaaca tcagtgtata
      181 aaattgcact ggcataaaac attctgagta tatttaaagc gtcaacttcc gaaatttcat
      241 cgctcgtttt ttcaatagta cagtctgcgt cttcgtaata ttcccttatc cattctccta
      301 gatcatcata tatgatagac atactttcaa tgctgttatc atctgagccg gtaatagtgc
      361 atttaagttt tgttggtgtg ttgttaacag ttatagtgca gataacctca atgtttctgg
      421 tttcagtttc attaatgctg ttttcgtcat aatcaaaaac agacatattt tcttttgcat
      481 aatctaattt cttttttaca tttgttataa atgtatcttc atggacatct tgcgtaacct
      541 cgcttgtggt tagcgtggtg gtcgcaacct catctgacgt tgttgttgag atcgttgttg
      601 ttgtgaaatc ggtttgctgt gaattattga ttgatttatt gctgctttta gtattgtttc
      661 cacaagaact taaaatcaat gttgaaataa caagtgataa tactaaataa ccattcttca
      721 taagaaattc ctccatattc ttctatagtg ctttttttga cactatttat tttgtactgt
      781 cattatacca cattatttcc acgatttgtt aagctggctg cttaggataa cgattacatt
      841 ttgtaaacaa aatgtaaatt ttgcagatgt aaacgttgat agggggtgct tttgaaataa
      901 ggggttatgt aaaaaaagct cgcacatatt tcagattcac gattcggcaa caggatatgg
      961 tgctgaatag tggctgatga tatgtacgag cttatagctc taagtggttg attatgtttt
     1021 tatgtatgtg gagcagaaac ggttatggca agaaaactat caccagcaat tacggcgaat
     1081 gcgaaatatc cgtaccccgt gaccgcaatg gtaaatttga gcctaaagtc atcgaaaaaa
     1141 gacagactca cacagatgaa attgaacaga aaatcatggc aatgtacgca aaggaaatgt
     1201 cccagcgtga cattgaagat accctaagag aaatctatgg tgcagaaatt tctcagggac
     1261 ttgtttcacg cataaccgac ataattcttc ctgaggtaaa tgactggcag aaccgtcctc
     1321 ttgacaaaat ataccccgta gttttctttg agggaattgt tttcaattca agaaaagaca
     1381 acaagatcat cagcaaatgc gtgtactccg tgcttggcat aaacatggag ggacatggat
     1441 attcgaaaat gaatccgcaa gtttctacgc aagtatatgc tcagacctga aaaaccgcgg
     1501 tgttacggac attttcatag cttgtcacga taatccgact ggactttgtg atgcaataaa
     1561 tgcaatattt ccaagggcaa aaaatcagct ctgtatgaca gatttgcagc ctgacggctg
     1621 atgagggttc caccctcaaa ctcccgaggt ttattcgctt tgggaatatc cagtgaagat
     1681 gaagaaaagc agcaacattt ctgtcattgc tcttcaccgt tttattccgc agcctgcgtc
     1741 ggggcactcc tcagcgttgc cctgtttgga ctgcatcttt actatggtca gaattttggc
     1801 gattattgcg tttatacggt tattttttca tacccgtaat acctttacaa aatcaaaatt
     1861 acaccaaagt tcttttgcaa atgtcaataa cttagcctta cgctttttta tggattccaa
     1921 cgtccacaca tcatcaatca tgaattcttc aagctcattc tggcacttat atggtgcttt
     1981 cgcaaagtat tttgagttct tttcatcaac atctttttta cctttacttg agttagccga
     2041 ctgaggatca agtgttagat ttccaatgct attgaggtac ttttcatcaa acacttcaac
     2101 agaaccaaca tctattttaa gcgcatcagt aataacctta gatttttcgc ctgaattgct
     2161 ttgcgcaaca atatgttcga ttgtcatgac gagcttcttg ttttctttct gacgtaaatc
     2221 ctcgtgtgtc attggggtag caactggctg ctcagtacgt ctaagatagt tttcatattt
     2281 ccagtagaag tagttttttt cggggctgct gtacttggtg caaaaatcgg gagcagtaag
     2341 tgcctgaata aaagcatcat cactctcaac gtccttgatc atggctatta tttcatcaga
     2401 agcatttttg aagttgccat caaagtctct ggcaaggctg taccacttac tttgcaattt
     2461 gtttgccatc ttatcaaaga taacatatac tctgaaactg aaaatttcac acagtctgca
     2521 tataatccta aatttggatt tatcattagt atcatcgtat ctgtaacact taatcaaaag
     2581 cggcatgaac gaagcagttc tgtcaagagc tattatatcc atgaattcaa ggtaacgacc
     2641 ttcatacatt gatttgtaag ctgataagct ctgctgtaca tttgtaagta tagttttcta
     2701 tgtagtcaga taataacggt ttatcttctt tagcgccatt gctgtttcga gccaattctt
     2761 cgacagtgtt cttgaaatac tccatatatc ttgtatattc tttgatacta ctgtcagttt
     2821 tccatttttc ataagcgata tagttgtact gcaagacgga gttttcatca actcctatat
     2881 taagagaaat catgctctcg aagtcacgat aaacttcact aaaccttgac tgtattttac
     2941 cgagtatttg ctccggttca tcaaggcata acgaagcctt atacataaga aagctctttg
     3001 ttttttccag gttagtgagg ctcttgccac gatcatttgt tgtttcaaaa atcatagcag
     3061 cttcgatact atcctgaatc agataaatca aaacgttagt tgagcatact ctatcaagca
     3121 gttcctcaat ctgttcgtct gaacaacact tgatcttatc ttcaaaaaaa gccttagcat
     3181 acaaaagtcg tttttgcgca ggggttttgg tttcacttgc ctttttatca tcaaggatca
     3241 cagtcgaaaa gaagtcgtta tcatctgctt gcagttttaa tatgcagaga tccccgttct
     3301 ttatgaaatg cttgtacagc atagcggttt cgtcatattc tatcgacgat aaccttgaaa
     3361 tcaagcattt cataaagata atcaaagttg taaaacgctg ttgaccatca acaatgtcac
     3421 aaaactcttt tttatctttt ttcgtcattt gaagaagaat tgtgccataa tagtattcct
     3481 gagaattata gttattcata tagtcctcga agaaatccct caactgcttt tcttcccaag
     3541 catagctgcg ctgataatca ggaatagaaa agaaagtttt gccgttgaag atattatcta
     3601 ttcttaattt tccattgtcc atcgttacac ctcctattat tcttctgctg ccttctttaa
     3661 ggaattatag atttcttctg tgaaaattcc aagaacttca cgcttgattt cctcatcgtt
     3721 caacagcagg gagaagaaat cctgattttg ctctaagccc tcgatcaagg catcgtcaat
     3781 atcatcgaag taagagaact caaagtcctt tacagtattg ttctttgcac ttgctttcag
     3841 cttatccgac ttcatgagaa tgtctctgat ctggagcata gcttttacag caacatcgct
     3901 gtcataggct tttccagtcc gagcatttat cgctgcaatg atttctgaga gtctttcttc
     3961 ctttgcaggc gttaatccga agttttcggc ggacggcagc ttgacaacag gctttgcgac
     4021 aatatcggat tttgtatgtt cgtcctgctt cttttgaaca aactgcgtag ctctgatctt
     4081 gccgtcaaga ttgaagcctg gtcctgaatg cttgatattg atatgagaaa gcaggagaat
     4141 aatatagtta tacttcttgt gaagctctat atcctcgaag ctcgatacct gaatcatgaa
     4201 ctcatagaaa cggacgaagt gtcggagatc aacaacgatc tctttctggc gttctatgct
     4261 gtaatgctcg attaacttct gagacttgtc aaggcagaag ttgactttct tcttatctgg
     4321 agcaagtact ttttctttgt atagtatctc atttacctta tccacatctt cgggatcaat
     4381 gattgcataa gcatcaatct gagcttcaag atcatagata gctgtaggcg ttaccgagtt
     4441 ggaaagaagc gtcgttgtat aatatggagc aaatgcaccg attatatcct cataggtgtt
     4501 cacaaagtca agaataaatg tctttttctc aaacggaggg cagattctgt tcaggcgtga
     4561 aagtgtctgc acagcgttta cgcccttgag cttcttcatt acatacattg cacagagttt
     4621 cggctgatcg aatcctgtct gatacttatt cgccacaaga agcacttgat aatcgtcttt
     4681 atcgaactct tttgtgagcc tatcttcagg aataccgttg atacccggct atgtgtattc
     4741 tgtatcatca tcagggagct tcaccttgcc agaaaacgca acaagcgcac gaatgccctt
     4801 gtatcccttt ttagagcaat agtcctcaaa tgcttgacaa tacttgacgg cggcctgtct
     4861 tgatgcggtg atgaccattg cctttgcctg tccgccaagt tcctgcataa cagttgtgcg
     4921 gaaatgctct acgatgacct ctatacgctg ggcaatattc gtttcgtgca gctcaacaaa
     4981 ccgagcgatc tgccgtttcg cacttgcagt ctgcattttt ggatcttctt caatttcctt
     5041 gtttatctga tagaaggtct tgtaggtggt gtaattctgc aatacatcga ggataaagcc
     5101 ctcctcaata gcctgcttca tcgaatacac atggaacgct tcacgctgtc cgttgggatt
     5161 gatcgtgccg aaaagctgta aggtagtcgg tttcggagta gccgtgaaag caaacatcga
     5221 aacattctgc tgtttgccgt ttcttgcaag ctctgcggca atctcgtcct ccatatcctg
     5281 atacagctca tcacccatac cgagggactg tgtgactgct gccatatctt ttccggcagt
     5341 agatgaatga gcctcgtcaa taataaccgc aaagtgttta ttcttcaagc cctgaacact
     5401 gtccacaata tacgggaact tctggatcgt tgttgcaatg atcttcgtat tgccgttgag
     5461 ggcgatagcg aggtcggcag aattgcactt ttcgtccatc acacggataa gccccgactt
     5521 atgctccata cccatgatcg ctttctgaag ctgtcggtca acaaccactc tgtcggtcat
     5581 gatgataaca ttatcgaaaa tgatcttgtt gtcagcatca tgaagtgtcg caaggcgata
     5641 cgcaagccaa gcgatagtgt tggtcttgcc ggagcctgca ctgtgctgaa taagatagtt
     5701 gagcgaggtg cggttttcct ctacatcggc aaggagcttg cggacagcat cgagctgatg
     5761 ataccgggga aaaataatgc tctccttgac ttcgaccttt cctgtctttt cgttctcttt
     5821 ctccgaggtc tgaatgaaaa tgaatttgga aatgaggtca agtatcgtgt ctttggtgag
     5881 aatatcgtcc cacatataat gaacgggata atcgtcctca cagggtgcat ttcctgcacc
     5941 tgcattgaca ccctcgccgt tgccctgatt gaacggcagg aagaaggtct tttcaccgtc
     6001 gagcttcgtt gtcatataga cttcgtgcaa gtccatagcg aaattgacga gtgtgcctgc
     6061 cttgaataag aacagtctgg acttcggatc acgctccata cggtactgat atatcgcatc
     6121 gtccgttgac cgtcctgcat agttgcattt cagctccata gagatgatcg caaagccatt
     6181 gaggaagata acaacatcaa ttcgctcttt gtcgttcgcc cacacttctt ccatgacggt
     6241 aaagcggttc atttcgtatt tccgcatcag atctttattg aaagtggttg caggctttgt
     6301 gaacatcagt tccagcttat gccacgacag ttcgacaccg tgacggagga cttcaagtag
     6361 gctgcccctt ttcttcgtac actccgcatt gatgaacgaa acaatggtat cttcgagcct
     6421 gtccttatgt atcttgcgga gcttctccat tgtctcaggc tgggtatcat tcagaaagcc
     6481 aaacagcatt tcacggtcga tcgcaaaacg gcggtcaaag tcggtcgctt ttctgatcgt
     6541 gtagccgttt tggtcacgaa gtatgccgag cagttcagct tgatattgtt tttctgttag
     6601 tatgtcattc atgcnnnnnn nnnncattca tgctgttatc ctccgatcca ttctccagtt
     6661 tcaagattta caaaattagc gagtttgatc tcagatacaa aaccgtcgcc atattcgccc
     6721 ttacgtgtct gatcatcgaa cttaaacgag atattgtata gctttccagc cttagttttt
     6781 cggataagac cgttttcaag gaaatatgtg atacagcgtt tatgagcttc tttatcatcg
     6841 aaatgaagat agaaacagca cacaccgtca ggagcgttgc tatgctttgc ttctgccaca
     6901 acgccatcgg caaccgcttt ctcgcatatt ttagccgcaa agtcattatc tttgaaaaaa
     6961 tacatccatt ttccgacctt gctgtcatca aacttcttgg cttctgcttc gttcacataa
     7021 tacacccaat aggcatttga gactttttta agcaatttag ccataccaca cacctccatt
     7081 taattggctt tcagtcgttt gattagttgt tttgcaaaca gcagagcgat ttgatcgcaa
     7141 ttatatttgc ttgaatcgat tgcctgaata tcagccacat tcggatactt ctcctgaagc
     7201 tgttgcatgg taatattgtg tactatgggg agaatcaact tttgtccatt acggttttga
     7261 cggttcagaa actcagaaag ctcacgctct gtccattcac ggtcaaagaa attctcggaa
     7321 ataacaataa tagcaaactc agccttcttt gtaccattca aaatccgctc tttccagttg
     7381 tcgccccatt caagtgattc tttgtcatag aagatgctta tccctaactt ttgaagtgac
     7441 tggtaaagtt tctcaatcag atcctcttta tccttattag cgtgggaaat aaacacatca
     7501 tattcaggca aagttttagc ctgatacttc ggaacttcct ccttttccac tccattcatc
     7561 ttatcaataa aatctctatc tttgcttata cttgtcaatg aagcaactaa acggctaaag
     7621 ctcctatgga ataaaagcga ctccattgac gaatacaaag ggtgttcact taaatatttg
     7681 gcgcggaaga tcctcacatc gtccatccac acattggcag gcatattatg ctctgcaata
     7741 ctttcaccgt cataggaaac ttgtgtgggt ggtctgatgt tttgggctcg ttcaagtaaa
     7801 tcagtaagtt ctctttcaaa cgctcccata gtcagttcac gttcagcttt ttctcgttct
     7861 tctttatgct gctgataaag gtagccatct tttagcacct gaatgataac ggggatattg
     7921 tcagcccaag tagtgtgaac caagttatta tctttcagct ccttgaaaca gccacgaagt
     7981 ataatatcct ctcgatggtc ggctgcttca aatcgttctg cccaatggtt tccgtcagac
     8041 tcatgttcga taatctcaaa caggaggttt tcagcttctt ttgagatcat aatcaagcct
     8101 gcacctcctt cttccccgtc acatactcaa atatcagcga cttcttgtat tcctctattg
     8161 tttcgatctg ctgtttcttt tcggtaatta tgttgtcaat ctctgcacac ttactgtcaa
     8221 gatagtcggc aattcgttgt tgttcatcaa gaggaggtac agaaatcaga atatttttca
     8281 tatcctgcca acctgttgtc caaagatcat ctactattcc atgtccccaa cgataaaatt
     8341 catcagcaaa tccaacagta tggaaatacc agttgtaata tcgaggattc atttcagtaa
     8401 tcgggaataa taccgtattg atgagcgata cagagccagt ataatctgaa atcccacaag
     8461 aacctctacg atcagaacga ctgtttatta caaaatctcc tttgacaatg agttttctat
     8521 cgtcgtgagc atttgttttg gcaactgtat ctaattgtgg taatacgccc attttagtaa
     8581 cagataaggg cggataatca atatcactta ctttttgtgt acgaagtcca tataaactac
     8641 caatacgaat taaagtccag tgcgtaggta tttcaccaat ccactgcaca ccgctattcc
     8701 tcatctccac atcaggatca agcccctttg tgaccgcttc ggtgatgacg gaacgcttgt
     8761 attcttcaag ggcttcgatt tgggattgaa tgtcggagat tagagagtca atctcagcac
     8821 attgttcatc gagaaaatcg gctattgctt tttgttcagg tatcgtagga agaatataat
     8881 ctatttctcg aagtttcttc caatttgtat cacaagttct tacacggatg cctgttgcta
     8941 aagataggaa cagctcacag tatgccattg tccgcattaa atacatcata tatcgtttat
     9001 tctgctctgt gtcaagcaca tttaatacgg gtgtagcttt tcctcttgaa tccgagatac
     9061 caatagagcc agcaaatccg tccataccat gaaccaccaa gtcaccaatt tcgataccct
     9121 gatagcctgc ttcggtcaaa gaaacagtaa aaccttcttc tctcctttta gaacgcagag
     9181 taacctcacc atcacgaaag caagttatta cttcatcagt ttctaaagta gggcgagtta
     9241 gtagttccat aaaatactta cccttgttta tgttccaagt atttgggatt actcctgccc
     9301 actctatccc gctatccttc atttcacgca ttgctgttca ccgtcctatg cttcttgaaa
     9361 aatctcatag ctgtcaccac cagtgtgcag agcatagcgg cacctgccac accgcctgca
     9421 atcataagaa tgatctgcca gtttgcgatc atgaagtagt atccctgaat agcaccgtag
     9481 gcaagaagcc acagacccac aaagagaata agtctgatga gccaatggaa gaacgagcct
     9541 tccgttctgc cgctgataag accgccatgg tacatatcac cgaccttgct gtatgcgata
     9601 ccataggcga ccgcaccgat cacagcgaga ataatatatt cgtaaagtgt attgataggc
     9661 agaccgaggg gatcggtcgc tctctcaaac aaaaacttaa aaatagcgct cattatagct
     9721 cctttcatta gccaaacagc cgtgcgatac gctcactcac agacttttca agctccaaga
     9781 accgctgttc aagctcctcg ctgggcttcg gctgctggta cttatagaaa taacgggtaa
     9841 acggtatctc cgcacctgtc ttgacaacag gatttttctt gcccaaatct tcctcaaaga
     9901 accactgggc atcgggaaca tggggaagaa cctcacgagc catatagtcc tcgatactct
     9961 cctcaaattt gacgatctcc gtgtcctttg tctctttatc gtagataatg ttgcctttct
    10021 tatcacgctg aatttcggca gatttgtcca tgatcgaaag tccgtcagca atcttgtcaa
    10081 ggagcttctt gtctgctgtt gccgtgctga gtatctttgt cagaacgggc aggaactccg
    10141 caggggataa atatacctta tcatcatcgg cgcatctcag cagttcgatg atagcttcgt
    10201 aaacatgtgc gttgttttgg tagttttcga gctttttcaa ttctttgccc gtcagctcct
    10261 cctcagagtt ttcaagctct gccacctttg caggatcgta cagtgaggag agcgaaccct
    10321 ttgacagcat tgccctgata cgctcctcgg tcacggcata gcttctctgc aacggctgca
    10381 tgaccgtgta ctcacgatag ataaactccg tgtttgggta gatcttgcaa agctcgttct
    10441 cagcaaagtc tgcatacagc ttcgtgatag cggaacggtc ttcgggactg atctcgttct
    10501 tcttgtttcc aagagctttg cgcagcttgt ggaagatcga acctgcatca atgagctgaa
    10561 ttttaccacg gcgctcctca cgcttgttct tcgacagcac ccaaatgtat gtagcgatac
    10621 ctgtgttgta gaacaggtca acgggaagtg cgatgatcgc ttcgatcagg tcattttcaa
    10681 gtagccaccg tctgatctgg ctttcacccg aagaagtacc gcccgaaaac agcggagagc
    10741 cgttttcgat gatcgcagcc cgaccgaggg cattgtccat tttattcaca gccgactgaa
    10801 tgaaaagcaa ctgcatatcg cctgaacctg gcagtcctgc accaaagcga ccgtcaaagc
    10861 ctttctggtt ttcttccttg acagcctgct caacaccctc tgctgcgtcc ttaccacccc
    10921 acggcgtacc gaacggcgga ttttcgatta cgaaacgcat ctgtctgtca gggaaacagt
    10981 cagctttcat agtgtcctgc atacggatat tctcggcatt ctgccccttg ataagcattt
    11041 cagccacgca catagcatag gactcagggt tgatctcctg cccgaacaga cgcaccttcg
    11101 cagtcgggtt atagcgatga atgaagttat atgaagtcga gagcataccg cccgtgcctg
    11161 ctgcctggtc tatgttccta ctgattttga tacaatagaa aaagccttct ccggagcaaa
    11221 agccgccgaa aaaggcttga aataagggct tccgaggtca tacgctgact gtggaagccc
    11281 ttgttttatg tgtttagttg tcctgctgcc gctcggtaat acggcgggat gccagccttg
    11341 cggtgtagcg attttggaat aattaaaagg tggggcaaaa ttaaaaggtt caatctgttt
    11401 caaggagttc ccttagtgtt tttccatcag cgtctttcca gtgcacattt ccgctcaaag
    11461 acgcgccacc aacaaatccg gcagctgatg aaggactgga aagaagtaca tcagctgtta
    11521 agatcccatt gctgtcaatc ttgtcctcat acttcttacg gtcctttatg gctctttcag
    11581 ggcagctctt cgttatggta ggattgatta tgctgccctt cagcacaaca aatccatcgc
    11641 ttgttctttt gccggaagcc ttgccttttc catattccat gtataggact ggttctgtgt
    11701 cagcggattc tgcagaaggc gcaaacggct caaatacctt atgcccaaga gtgcccatca
    11761 caattttcgc atagtcgata aactcttcaa gttcgctctc tgtctcctcg gttatattcc
    11821 ctggattggg gtcatttccg tttttaacaa catagcgacc tgcttcgatc gccatattgc
    11881 agaagcgatt ttcaagatag cttatctccg tcggtccgaa agagttgttt gtcgtagtga
    11941 acatgaccgc ctcggtccaa taatcaatgg agttatgggc ctcttgcact cgcagaagaa
    12001 gccctttgcc atttttacgc actccagcct ggcctatgta taccactgca ttatccgcct
    12061 tatcagtgcc aaacagaaaa tacacaccac tctgtttgag aatatccaaa cctttgcact
    12121 tgtccaatgc agtgcgaggg attttatatg caatcccggt ccagttggcc aaggagcatt
    12181 taatccttcc gctcggctct ccatccatca aaaatagttt taaagtctta ctcctgggca
    12241 tgttattttc tccttaatcc atgagaatca taaaaagttt atttattgtc tcaccatgcc
    12301 atctataccc atctggatat aacttgcacg attgctcaca aggtacttga agctggaatt
    12361 cagtctgact tcatcatgtg agataagcgc atactcccac ggctttccac cgttagcggc
    12421 attccattca gataccgctc tgcaatacac aacagcggca gccgcttttt cttttacgga
    12481 tgagctgtcc atctcgttgc ttgccttggt ttcgaccata tagattttat cttcggtctc
    12541 aacgataaaa tcaggctcat acttgctcac gccgcccgga ccgtagtaga tgttaaactg
    12601 cttcggagac ggacgcatcc actttaggac atccttatcg ttctccagta cgattgcaaa
    12661 aatacgctcc gtgttgctgt cgaacttata gagtgtgtga caagcctttg tgaaaccatt
    12721 aaaaatcttc tgccgtactt cgcctgccgg gatgttggcg cgtaaatcat aaatttcatc
    12781 cgatcggaat ttcccaccga aacttgtctc tatccgtgag aacggtcgca tttccgatgc
    12841 tctgaaatgt gtttcgtcac ggaagaaatg ctgcttcatc tgcgcataga taatttccgc
    12901 gatagttttc tgcctgtcac gcataacctt aacggcttcg tcctccgtaa ggtacgacaa
    12961 aaagtgctgt ttagcttcct caacaagtga atacagcaaa tcggcagttg catggtaatc
    13021 cacatcgtcc ttaacgataa tgtgtcggac gatttcattc tccggcgtat ccacaacatc
    13081 tcttgcatgg aagtcgctct cgtattcgaa ggtgcggccg ccctcctgga gttcggttcc
    13141 aatcattgta tcatcggacg gatgccagtt catgcttgat gtaacgagtt tgaacggata
    13201 gaatccctgc ttgacttctg taaatggctg aacaaccgcc tgcggaatag gtatgacatt
    13261 atccgtcaat gcctgcacac aagtgtcaat tgctttctgt acaacgactt caaggtcttc
    13321 acggctgaga ttcatctccg ggaactgtcg gatcgtttct gcaataacac tgttctgtac
    13381 gaatttgcga gtttcagggt cttttgccgc atcaaaggtt cgcacctgac gattaagctc
    13441 cacgaccgat cgtgaagcaa aattcgccac gaaacttgcg atttgcgctg cctgctcctg
    13501 agtccgaggc tgttccgttc cgtaatgagc agttgactcc tgcacgaacg tcagcgccag
    13561 ttgctccata taactcgtgt ccgatgtgag ttcatcatag gttgtcggca tttccaccgt
    13621 aacccgctgc tcgtcggatg aggtcgtttc agcgggatcg atataataaa cacgccttac
    13681 gagagaattc ggatcattgg cgagattcac aattgcctca tatttatcgt ggctgacgat
    13741 ggacagacga tccacctcgt caacacccgt tctgtcgccg taaggaagac gcagtcctct
    13801 gccaatagtc tgctccgtta aggtttccga ggctgatgcc cgtaaaggca ctatcgtgta
    13861 gagattggtt acatcccagc cctccttcag catatttaca tggatgacaa tctcgatttt
    13921 attatccggc ttttcaagag aaagcaactg ctcgatgttc tcgtcttttt ccgctccgcg
    13981 ctgtgaagaa ttgatttcta taaccttgtc tttgtaatag ccacggaaaa agtcatccga
    14041 ggtgatatac tccctgattt ttttcgagtg gtcggtgtcc ttcgccacaa cgagaataaa
    14101 cggcttcaca acaggcctgc cgaaagtccg ggcgtaaatc tcaaggtggc tctttgtctc
    14161 ctcatgcaga cggacaccgt cattcaactt ttcatggtca agctgttcgg gcgtatattc
    14221 ctccggtctg aaatctttac gagtgaagac cacaggcact ttgacatact tcccgtcgtt
    14281 cagcgcatgg gcaagcgtgt attcatagac aacattcttg aagtctactt ttcgcgcacc
    14341 tttctgaatc tggggagtag ctgtcaactc cacacccaaa atcgggcgca gctcattgat
    14401 gacatcaaag ctcttgtccg catggtagtg gtggctttcg tccatgaaga tgcacaagtc
    14461 atccagagat tgcaagtacg caaaatagga ctccccaagc acctcgttca aacgcttgat
    14521 gcgggcagga ctgccggatg agaccttgct gtcagagttc agcttggcaa tattgaagat
    14581 gttaataacg acgtcattca cgccgaaggt tccctgacgg aagtcattat agttatcgcc
    14641 gtcgataata cgcgggggac ttacgaattt atccaaacct cgaaacacat acttcggact
    14701 cgacggattc ccaaggtcat ccttcagttt cctgtaaatc gtgagattcg gggccatcac
    14761 gaaaaaattc tttatgccct tctcgtagcg gagatatgca atgcaggctc ccatgagacg
    14821 agtcttgccg atacctgtcg ccagggcaaa gcagaacgat ggaaagtcac gctcaaacga
    14881 cgtcaaagtc gggcagagcg cacgagccgc agccaagtca tcggcgtaaa attccgcagc
    14941 cgcctccgat gcccattcgc tgattttctt cgttctcttg tcctcggtat caagtgcctc
    15001 gagctccgcc tgatgtttgg aatgaatctg ctccgttgtc gtaggatttt tagccaaact
    15061 aagcgtgtca cagagtctgg caaaaatctc caggctttca tgctgcggag gacggagcgc
    15121 aagcacattg tttatgtact tggaattgta cgagtagaaa ttttcggtat aagattcctt
    15181 aatcatcttc ccattcctcc tcgccgcatt ccggcacttc cacaatgttc agattatagt
    15241 tgtccacgcc gtactcgcac ttatcgagga cggactgcgg aatcttcttc acggtgatat
    15301 tgtcgtagcg tttattcaga cccacatcga aagccggtac gcaaatcagc aggctctcga
    15361 aagcgtccac ttctttggcg aggctgtcca gcatagccgc cgtcagatac tgcgtcgtga
    15421 cgtagataaa gctgttgtcc tgactgtatc cctgtttcca gaaaacgtcc gggtctggag
    15481 catagaagta accatttacc tttgcaactg ccgccacaag catctgagca ttgtatttat
    15541 ccgaaaatac atcattgcca tgctcgtcct taacaatcaa tgtcggtgca agttcataga
    15601 acttgtatcc accgccaccg tgccagttag cagaagaggt tattcctctg ggatcatcat
    15661 catctatcac cctgtccaaa cgcaccttac agtgagaata ggcgtgatta cccatttcaa
    15721 tgcctatcca ggcacgattg agtttgtgtg cagccgcagc tgtagtgcct gatcctaaaa
    15781 aagagtcgag taccagttct ccaggggcag tggtcatctc aataatctgc ccgaccaatt
    15841 gctccggttt ctttccgtct gggaagtcaa cgccgccttc ttttgcaaca ttcttcatcc
    15901 aggcattcat gtcccaatag gttccctgca aatccttttt gaacagttca ccatcgatta
    15961 cctcagatgt gtctctgagc catacaaaca atcggcattt gtctcctttg taaaactgct
    16021 cataaaccgt tccacggttt ttgcctgtct tcgggacata ttctatcgag attacatcct
    16081 cgacaatccc ctcctcgcgg cgatagtcca ttattcgagt gcggattgaa ctttgcgcat
    16141 ttgttgtttg gaaaatcgaa acaccatatt ttttatatgc ctccttctcg gtgattccat
    16201 ctcttcgcgc tacttgcttt attgacatca tgacgggatt tttgcgtaga taaactttta
    16261 tctcgtttcc gtctccatcc acagtcgagc cgtagtattc cttctcgcca ggttcaagaa
    16321 gaaccgtagt gtacttccag ctcttacctt catcaatata ctgctgaata agctcagaca
    16381 tttctgtgta agcataaggg ccgttgaata acggcaataa agaatagtcc ctggcatatg
    16441 caaggatata ttcacagttc ttcttcagcc gcttatcttc gccgccgcca gannnnnnnn
    16501 nnaccggcga tatttttcat gttgacggaa atcatattga tgaagttact tctaccgaac
    16561 acttcatcgc aaagcacctt cagatatgcc tgttcttcat catcaatctg aatccagatt
    16621 gtgccatcca tagaaagcag attacgaaga atagtgagcc gcgccctcat caagcggagc
    16681 catatcgagt gttcaaggtt gtcgtcataa tactcgaatg cagctcccgt attatagggt
    16741 ggatcgatat agatacactt tacctgtcca gcatactgtt gctccagagc tttcagtgcc
    16801 agaaggttat ctccgtgaat cagcatattc tcgctattgg gatcgccata atctttggac
    16861 ggatcgtgga gcagaatgcg aggttcgacc tcaggctcct gtccttttcc tatccacgta
    16921 agttccaatc tttgcattgt tttttctcct ttacacgacc tcaaacgaga tggtcatgat
    16981 atgccttatc tctgatgtgc cgttcaattt ttgccggatt tcctcctgca gcttatccct
    17041 ttgcatatca atctcatctt cctgctgata gatttcacgc tgcatctttt tccgtttatc
    17101 ttttaattta tttatttcat ccttcatgga gataatctga tcaagaggaa ggtttgtgct
    17161 tgatctgcat tccttttctt tttccttgat caacttgttt aactccttac gtttgcgttg
    17221 gagaccttcc ttcaaatcat cactgtatgc gtccagtttg tcgcattcat ccagataaaa
    17281 ctgcttgttc gcttcttcaa tctgctcctg ttggacaccg agtctacgtt cacgctgttg
    17341 aatgagttcc actgattcat cggggcattc gccaataatc tcagccggca gttcaagcat
    17401 actgttgatg atatcttcat ccattgttgt accatcttct gtaagaaccg taaaaataag
    17461 atgttcctca tagccaagac catcatagga cagcttatca attgacagaa cgcccttaat
    17521 gcccggatga gtattgaaaa aggaaatatt tctgtccgaa tccgtgtggc ggaagcgaat
    17581 ggcaacaggc aaaagatcag ccgacagact ttcctgnnnn nnnnnnactt tcctgcagcc
    17641 aagattcata caatggatct tcgcgacgga ggaaaacatc tccttcttct tcggcgtctc
    17701 tccatttaag gttatatgtt ctttttacat cgccgtcctc aaactgaagg cgccactgtg
    17761 taagtggctt tactcgttcc gctccgtgtg ccaggaagaa gtagtaaatc cactgtgtga
    17821 atttatcaag gctggctacg gtatcgtcct gacaatcctt aagccgtgca gctaccacct
    17881 cgtcaaagtt ttcaagaata gattgccttg cagatgccat tttatcttca atctcatcag
    17941 aaagttcttc ctgcaaagca tcgaattcat gctggatgtc atcagctgtc ttacactttt
    18001 gatagatctc ggctattcgc ttttcaaaat ccacaccgga ttccagagaa cccagaattt
    18061 catctgacga gccgaacacg ccatcaaaaa gccggaactt ctgatcaagt aattcataga
    18121 cgcgcctgtc tgccgcattt ttacgattta aaaagttgat aaccacgaca tcgtttttct
    18181 gcccgtatcg gtggcagcgt ccaatacgct gctcaatacg ctgcggattc caaggaaggt
    18241 catagttgac gatcaggctg caaaactgca ggttgattcc ctctgcggct gcctcggttc
    18301 ctatgaggat actcgcatga tcccgaaact cctccacaac agcggctttc atatccgcct
    18361 gctttgaacc agaaatcaag ccatcgtttt tatgacgctc tttccagtct gcataaattc
    18421 gctttgaaat ctcatcattg ttcgtgccgt tcaaaaatac tatttttcct tcatagccat
    18481 gattcgaaag cagattaagc aagtagttct gcgttctcct tgactccgtg aaaataacag
    18541 cctttctctg accacccagc tcttcggtct tagcaaagcc ctgttccaaa gccagaataa
    18601 ggtcatcacc ctttgagttt gtatttatac cggacgcaag ctcagcaaac tgcatcaatc
    18661 tttccagttc cgcttttatg ccatctctat cgcgcttaag atcagcggta aggatgtcct
    18721 cagcggcact atcatcttcg tcggacgctt catccattag ttcatcaaaa ccgtcataat
    18781 cgtccagaga aagctcagct tcaacacctt caagcaagtc attcagccga tcaatgagtg
    18841 actggagcgt acctgcgatg gcaaacgaag aggatgccaa caatttccgc agcaccattg
    18901 tgataagcgt ccgctgccca tcaggcaagg cataaagctt gtcggtttgc aaatattcag
    18961 aaacttcatt gtacagtttt tcttcgtctg ccgtaggagt atattctctc aggattgcat
    19021 ggcgtcctgt gtatctcaca tattcagata cctgcttacg tagtgtgcgc ttacagagtg
    19081 gggcaagtcg tcttcttaaa ttctggtttc ttatatcgct gttcgctacc gcaacataca
    19141 tctccctaaa agttttatca tccccgaaca cac
//
LOCUS       DAWEMR010000027        19133 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1194_length_19133_cov_11.362826,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000027 DAWEMR010000000
VERSION     DAWEMR010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19133)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19133)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19133
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1194_length_19133_cov_11.362826"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    332..341
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7187..7196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4991 a         4516 c         4075 g         5531 t
ORIGIN      
        1 gtgaacattc attttcaaac ctattgacaa agctaagtat ttgtgatata attaaatcaa
       61 ataaagattg taggctttga taacaattgc tgtacaaaac agcagtaagg tagggggaag
      121 aaaaaatgga tagtctgaaa acgtgttttt ttggcggcta tgataagctt gatacattaa
      181 aatatatcga caccataacc agcgagatct atatgctgga gtcggctatc gaaaagaaaa
      241 agaacggtga taactttgtt atccccggag aaacaggtca gcgaaagctt aaaggctcag
      301 cgcttggtgg atttgcaaag cctgatgtgg annnnnnnnn ntgtgcgctt cttggaaagg
      361 cggctgagct tcgtgaaatg ctttgcagct aacggctaat aaaggcaaaa aaatatccgc
      421 agaggtcatt ttatatggct tctgcggttt ttttgtcgca tcttgtctta aaagagcaaa
      481 aaaaaattaa tacccacttc catcggctga aatgagcatt aatttttata gaattatgaa
      541 agggattctt tggtatttgg taaaacctaa agacgaggaa atgtatcagc gacttgggga
      601 atcgcagagg gaaagaagac aaaaacctga ctttaagttt taccaattac caaagctttt
      661 tttgtatctt ctgctgtgat tatataatac cagttctgtg tgaaccgaat atgaattttc
      721 aggggatttc ggagagtttt ttgaaaaaaa taaatgttaa tcaccagttc acaattataa
      781 gctataatgt cattattggg gaattttttt acgagggagg atattttatg tttcagatac
      841 ttgtggtgga ggacgacgca agccttagaa agctcatgtc tgccgcactt aaacagcatg
      901 gctatgtgac ttttcttgca aatgacggtc tggaggcttt ggacgttctt gaaaaaacga
      961 atatagacct tataatttca gatataatga tgccgaatat ggacggctat gagcttacaa
     1021 gacagctcag aaaggctgat ttcaacttgc ctatacttat ggtcaccgca aaggagacct
     1081 tcgaggacaa gcaggagggc ttttctgccg gcacagacga ttatatggta aagcctattg
     1141 atataaatga gatgatactc cgtgtgggtg cgcttttaag gcgttcaaaa atggcgtcgg
     1201 aacattctct tactgtggga aactgcgtgc ttgattatga cgctatgagc gtggcggttg
     1261 acggcggagc ggcggaaact ataccgcaaa tggagttcaa gctgcttttt aaactgctca
     1321 gctaccctga caagatattc acacgccgcc agctccttga cgagctgtgg ggcatggata
     1381 aggacgttga cgagcgtact gttgacgttc acgttaaaag gctgagagag cgttacggaa
     1441 gctgtgagtc attcgatatt gtcacggttc gtggacttgg ctataaggct gtgaaaaagc
     1501 agtgacggat aagcttgagg ggtgaggggt ttggataaga aaaagaccaa aaataaaaca
     1561 agtttgcagt tcaagctgac tcttatgttt ttctttgtga tgctggtgtc ggcttctata
     1621 tcggtgtcag tgcttgtaat ggtgtgcagt cctgtaatga aaagcaatgc cgaacagcag
     1681 ttggtggagc tttccatggc aatggatcag cttgaagaaa agggcggctt cactgtggac
     1741 gagatagttt ccattgtgaa caattcagcc tatagtctgt cagtggtgga taaaaacgat
     1801 tcaagagtac acgaccattt tgcagatatc gtgcataagg gatattatgt gtcctcagag
     1861 ggcgttgtgc ctaacgcaac tataatctct caggtctatg ataagttcgt tatcatagac
     1921 agcttttcaa gcgacagcgt ttactgggta gtaaaccttg ttgtcatcat agcttttatg
     1981 gtctgtatcg ttatcggtac tattataaca gcgttcatga gccgaagcat tatacagcct
     2041 atacgagatc ttagtgcggc ggcggctgag atagcaaagg gcaattttaa ggtaagagta
     2101 agagagccgt ctgatcctga atacagcata cttgcgcaga actttaacaa aatggctgag
     2161 gagcttgcag gtacggaaac gctgagaggt gactttatct caaacgtttc ccacgagttc
     2221 aaaacgcccc tggcttccat acagggcttt gcaaagcttc tgcaatctga cgatataacc
     2281 gagcaggagc gtgaggaata cacgcagata ataatcgacg aaacatcacg gctttcaaag
     2341 ctttcgtcaa atattttgag gctcacaaag cttgaaaatc agaaaacagt gggcaaaaag
     2401 acaagatttt ctcttgacga gcagataaga aaaatacttc ttgtgcttga acctgagtgg
     2461 tcgaagaaaa aaatcgacct tgatatcgac cttgaggata tcatgtatgt gggcagtgag
     2521 gaacttatgg cgcagatatg gcagaatatc atcaacaacg ctatcaagtt caccccggag
     2581 ggcggaaaga taagcgtgaa gcttttccgc aacgagaaga atatttcggc ggagatatgg
     2641 gacaacggtc cgtctatagc ccctgacgta aaggcgaaga tatttgataa gttctatcag
     2701 ggcgaccgct cccgtgcaac agagggcaac ggtcttggac ttgcacttgt aaaaaggata
     2761 atcgagcttt ccgagggaag cattgaggta gaaaacgata tgaaaaaagg tggcgtttgc
     2821 ttccgcataa gcctgcctta tcttattgag gatatgatgt agtgtagggg caaccttggg
     2881 tcgcccgcct atcacagccc actatacata cgaagatttt ttgcaaacaa aaaggagcag
     2941 ttcattgcga attgctcctt ttatctgtct gtcatatttt agaacagact tgccacaaag
     3001 cctgctgcca caccgaaaac ataaagtatt cccattatgg cgaaattctc tttcatgttg
     3061 tgcttgttgg tcttgaaaag caccagaagc cccactcctg cccctgtgca aagtcctgcg
     3121 ataagcgagc cgaagctcac aacgccctcg atataaagct gagtgagaac aacagaagcc
     3181 gcacagttcg ggataaagcc tattatgcct gctatgaacg gctgaactat gctgtcagac
     3241 ataagcaggg tctttactgt gtcctctcct gcaaactcca ttaccgcacc gagaataagg
     3301 tttaccgcaa atataaaaag tattatgctt acagtgtggt gaagtgccga gtgcaaaata
     3361 ctgtggtgtt cgcagtcgca atcctcgcat atctccttga aaggctcttc gtcctgcttt
     3421 ttgcccataa gctttaagat aaggtcaaca agcacacctg ctattatagc cacagctact
     3481 tttataagaa taagctgcca aagcttgccg gcaaatgcag ggtttgctat catcattggt
     3541 attgcctcgt cgcttgtggc aaggtatacc gaaatgagag ttcccattgt gatgagcctg
     3601 cctgaataaa ggttcgacgc cacaacggaa aatccgcact gcggcactgc tccaagtatg
     3661 cttcctccta tcgcaccgaa gggtcccatt tttctaagag cctttccaag cttgtcggca
     3721 gaacgatgtt ctatatactc tataagcaga taaactccga acagaaaagg caggatctta
     3781 aggctgtcta ttaatgcgtc aagaaaaacg tcaactaaag tttcaaacat atatctatct
     3841 cctgtaaatt atctatggtt ttaaaacggt ctttatgtca actcgactat tatacaatat
     3901 ttattcagcc ttgtcaatct tatgtttata tgataatctg ttcatttgtt tacccttttc
     3961 agcaacaaaa aaacggacga aatttctccg cccgtttgat gtttttattc tgttttatac
     4021 agaacgcttt gtaaagccgt cccatgcggt gagtaggaac accaccatgt gtgctataag
     4081 aacgcctacg gcaaaggtca ggctgtgctg agcaaatatt gagtagccct ttgagataga
     4141 agcaaggtct gtgtttgcga acacaaggaa tcttgcccaa tcctgtccaa gctgacccaa
     4201 aagctgtgtg accgttgagc ctatgcacat tgtgaatacg ctgacgccga tagcaagggc
     4261 tgtgcttctt acaagtgacg agatagcaaa cgccagtgtt gacattacca ccatttcaac
     4321 gctcttcatc agatattgct ctgccgcata aagcagtgta ggcatttcct ttacctcgcc
     4381 gccgcttaca tagatataag gcgttgagaa gccgtcaaag ccgagcataa gccctgtaat
     4441 aggtatcatc accacgaaaa gtatgcaaag cattatgtaa cccactgtga tgacagtgaa
     4501 gtattttgac atcagtatct tccagcgttt cacagggttt ataagcagga acttgacagt
     4561 tccctgtgaa aattcagagg atacaatgcc gcctgcgata acgatagcaa gcagtgcgac
     4621 taccgttaca agggaagtgc ttgtgagaaa cactgtccag aatgttatct gctcaggctc
     4681 gtctgtttca aacagcgtca tttggtttgc tgtgttgtcg agcttatcat tgtcaagctg
     4741 atagagtgca agttttatat tatcctccag ctttgctctg ctgttctgtc cgtcagactt
     4801 tgcgtcgccc atggtcgcaa tggtgttttt tgcgtttgca actgtcatga taagatcgtt
     4861 tttcttctcg tttgacttgt caaaggaaat atccttgtcc aacctgtatt gatattccca
     4921 cttttcgccc tctgtgagta tgtcattatc cagcctgtat tgacagaagc cacgccaatc
     4981 gttgtcgtca agatacttct ttatccctga catatcacca gtgccgtttg cgaacacggc
     5041 tgacagtaca tcatatctcc aatcgtcgct gtcgatatcg ttgtcgatta gatactgcca
     5101 cattgcgatc tcgttagggt cgccgtttgt gtttttgagc caatctatgg tttcctgata
     5161 atcaccagtt gcgtcatagc ttgaatagtt attgctctcc atattgtgct ttgcaaaaag
     5221 tgcaatgcct gaaagaccaa gcgcacatat tattataagc acgatcatta tttttgttga
     5281 tagctttttg aaaaccttta tgtactcgtt tttcacaagt ctgaaaaatc tagccaatct
     5341 gtacgccccc ttcctttcct cctgtaagct cgatgaatac gtcctcaagc ttcttgttct
     5401 ctctgcggtg aacagtgtaa agtgaaactc cgccctcgat aagcgtctta ttcaccgacg
     5461 ctatcttttt cttctctatc tccacaggga tagaaaggac aactgcacct gtttccttgt
     5521 cgatacgctt gccctcgtct gcaagcttta taagactcat agccttttgc gggtcgtcaa
     5581 cttcaagtac atattcagca attgagctgt cagactgtga gataagctcc tccattgtgt
     5641 atgagcctat catttttccg tttgcgataa tgcccactct gtcgcacatc atttccattt
     5701 cgctcatgag atgtgacgat accacaacgc aagtaccttc cttgtgagca agctctttga
     5761 gtatgtcacg aagctccttt atgccctgcg ggtcaagacc atttgtaggc tcgtcaagga
     5821 taagaagctt tggtctgtga aggatagcct gcgcaacgcc aagacgctga cgcataccca
     5881 gagagtattt cgacaccttg tcattaatgc ggtttgaaag ccttacaagc tccaccacct
     5941 ctttgatacg ctcctcagtt actccctctc tcattctagc atactgacgt aggttttcaa
     6001 gtcccgagag atatttatac atctcagggt tttcaacaat gcctccaacg ttcgccattg
     6061 cgccctcaaa gtcttttctg atatcctttc cgttaatgga tatctccccc tcgtcaagct
     6121 gtaaaagtcc cactgctatc tttatagttg tggtctttcc tgcaccgtta ggaccgagaa
     6181 agccgtagac ctcgccactg taacactcca tatctatgct gtgaagtatc tgctttttgc
     6241 caagggtctt gttaagcccc tttatcttta gaatagtatc actcaacgct gtctgccccc
     6301 tcgctgtcgg tattttctgc actgctgtcc ggaattatct cagactcagc cttgctttca
     6361 tcagccacgc tgtcactgtt gttatcatca aagctgtaat cctcgccata tccatagtcg
     6421 ctggacgttg ctattttcca gccgtcagag gtacgcaaaa gataaaattc agcatattca
     6481 taactgaaaa tcgccttgta tgaagatttg ctaggctcat agttgtcgga atttgtgtag
     6541 ttaccgcttg tcatggtgtt tatgccgttg gttgtgagaa aagttgggtc gccctttgat
     6601 acgtcatagc acacattata tatcataatg acctttgctc cgtcggctcc actcttgttt
     6661 attgacatct cttttatgtc atactcagcc gaggtgatat ctcccccctg cgctgcaact
     6721 tcagcttggt ttatttcgtc cagaagtcct gactttgacg aagcgtactc attgtcagaa
     6781 gtggtgtagt cagtataatg cttattcaca aaatctgtca gttgactttt taacttatca
     6841 ctgcttccaa cgtttgcact tgtcaagtct gcgatataat ccctcatggt ctgttctatc
     6901 tcaggcttgg aagtcttgaa ctggttgtta tcatacactg agtagcatac aacgcccagc
     6961 gcaagcacac cgccaagaac cagacctctg tttatgtttc ttagtttcac agtttctcct
     7021 ccttgtgtct tatctttttc agacgtattt tttagtggca tatataagtt tatcatagct
     7081 cttttcaact gtcaaccgct ttaagaaacg cttaagattt tcataagaca accatacaga
     7141 ctataagata aaatacgttc tttgtttaaa tatctgtagt ggcgaannnn nnnnnnggcg
     7201 aacagttcgc tctccttatt agcgtatttg caagcaaagc aaaaaggcgg tggaacacgc
     7261 acgataacgt atcccaccgc ctatatggaa tgaggaaaat tatccttttt ataaaaacag
     7321 cttcaggtca ttctctgcct ttttcgggtc tttaagactt atgctcctta ccgcctttct
     7381 cagtggcagt acccttgacg gaactaccga cagcatatcc acatcaagct ccagaaatat
     7441 ctctgtgagc gttgggtcgc ctgctatctc accgcagatg cctgctttta tgccgtttgc
     7501 atgggcgttg tcgcacacca tttttatcat tctcagcagt gccctgtgat ttttcggtga
     7561 taccctgtca taacatgggt tctgcctgtc aagaccgaaa gcgaactgtt caaggtcgtt
     7621 ggtgccaatg ttgaaaaagt ccacctcttt ggctatgata tcgcttgtca tcaccgccgc
     7681 aggggtctct atcattgccc cgagcttaac attgtcggca aatttcttgc cctcttttat
     7741 aagctctgcc ttgacctgca cgataagctc tttcaccctc agtatctcgc tcacgtctat
     7801 gaccataggc agaagtattg aaagctgtcc gtaaacagac gctctgtata acgcccttag
     7861 ctgtgttttg aatatgcttt ctctttcaag acaaaatcgt atgcctctac agcccataga
     7921 gggatttctc tcagcactca tgtctgcaca atcaggtatc ttgtctgcac ctatatcata
     7981 agtgcctatt atgacctctt taccctccat tgcctccaat gccttgcggt aggtgtaaaa
     8041 ctgaaaatcc tcgtcaggaa aagcaggtct gtcaaagcac ataaactccg ttctgaaaag
     8101 acctatccca cctgcgtcgt tttttaccgc cgattcaagc tcctcagagt tgagtatatt
     8161 cgcatatatc tttatctcat gtccgtcacg agtgatgttt tttcttcctt tgagccttgt
     8221 gagaagttcc tttttcctgc cctcaacggc ttccttgtat tcaagctgtc tgagagtatg
     8281 ctcatcaggc tctatgtaaa gcgttccgct gtagccgtca agaacggctt ccttgccctc
     8341 atattcgtca gaaagctccg ctccaacgtt cacaatggca ggtatgccaa gatttttcgc
     8401 aagtatcgca gtatgagagt tcacagaacc agaccttgtg cagaagccct ttatcttcgt
     8461 cttgtccatg agcagggttt ctgagggcat aaggtcaccg gtgcagataa tggcattctc
     8521 tctgagtatg ggaagcttgt cggcacagtt taaaatgtgt cttaaaagtc tgtttgaaac
     8581 gtccttgacg tcagccgctc tgctttgcat atattcgctg tccatttgtg ccagcatctc
     8641 cgcaaagctt cttgaagtgc ttgcgactgc ataatcggca ttgaaatgct cgtcaagtat
     8701 ctttgtgcga atactgctgt cgtattgctc gtcagtgata agcatttggt gtatcacaaa
     8761 tatttgagcg tcgccttccc caacgcttaa ccttgcacgg tcataaagct ccccaagttc
     8821 tacaagggca gcgtttttag ccttaacata ttttttgtat tcaagttcag ggtcagacac
     8881 ttttatcttg ttgatgataa attcgtcacg atgataaaag gttagctctc ctcttgcaat
     8941 gcttccgaat accgatcgtc cttttatgct tgtcatgagc gctcaccccc aaaaacatac
     9001 ctatctgata taattatatc accctttgat taacgataaa agtaaaatgc cgtctgttta
     9061 caaaacgtta acaatattcg tgattttagc aaacaagaca atcaatattt cagcaaattt
     9121 aacaaatcag aaacattttg cgaccttatg tcaaaatcag aatagtacaa tccctaaaaa
     9181 tcatatgatt aaaaagcgta tgagcgatac tttatataag gagggacaaa tatgttttca
     9241 gcactgtttg aactgcttac ggggaatttt ctatttttcg cactgcattt tgacagccgc
     9301 agcgtgtttc caaaaccgct ttcagcgaag gaggagcgtg agtgttttga gagaatggcg
     9361 cagggagaca aggcggcaaa ggataagctt atcgaacaca atctccgtct tgtggcacac
     9421 attataaaaa agtattattc aaattccaca gatcaggagg atcttatctc tatcggcact
     9481 atcggtctaa taaaagctgt gtcaacattt gaccacaaga aaggctgtcg cttcgccacc
     9541 tatgggagca ggtgcgtgga gaacgagata ctaatgcact tccgctccca gaaaaagtct
     9601 gccaacgata tccactttga cgagcctgtt gactccgata aggacggaaa tcagctttgc
     9661 cttatcgaca taatcgcaga tggagaggac gttgccgaca aaatagagct ttccattcgc
     9721 agtgaacagc tctaccgcta tatcgaaagc tctcttgatg agcgtgaaaa acagatagtc
     9781 actctgcgct atgggctttt cggtcagccg cagctgccgc agcgagaagt cgcaaaacgc
     9841 cttggtatct cacgttccta tgtttcaagg atagaaaaga aagccttgaa gcttcttaaa
     9901 gaccgcttcg ataagcagtg ataggcgttg acgcagtagg ggcgaacagc gttcgcccgc
     9961 tgattatcat atcatgtcat gacattgttt tagcactcac gaaatcaata tgctagcact
    10021 cgcataatga tagtgctaaa attcagctaa tgttcacaat atcttcacac aatgaacaca
    10081 tagcaggggt atcataaaga caatgaagaa aggaaatgat accaatggaa gaacagaata
    10141 aatgataaaa gcttacaaga cagtttaaga tataacacat caaggaggaa tttatatgtt
    10201 tggacttaca ccatttgaaa gaaagagcta cgacctgttt aacgcattcc atgattttga
    10261 gaatgatttc tttggagaaa atgcactcca gagcttcaag accgatatca aggatgaggg
    10321 cgacaaatat gtaatggaag cagagcttcc gggctttgat aaggacgata tcaagcttga
    10381 catctcaggc aacgatcttg ttcttaccgc tgagcataag gcagagacag aggataagaa
    10441 agacaagtat atccgccgtg agaggacata cggttcttat cagagaagct ttgaccttac
    10501 aggcattgat accaacaata tcgccgcaga gtacaagaat ggtatcctga tccttgacct
    10561 gccaaagatg caggaggtaa agcctgaaac caaaaggctt gagataaagt gatttaagta
    10621 aagccaaagc tttgaatgag gcgataccaa tgggtcacga tatgatttgc aataggtttt
    10681 gctagaatga aatatccgtg agagcgaaat gcccccacgg atattttttg ttatcacgat
    10741 tttttctcac ggcttttgag ccatattttt cgtattagta tggcaagtaa aattcctgcc
    10801 actgcaccgc ttgtgtcgat aagaacatca cgcacctgcc ctgcacgccc tggcacgaaa
    10861 agctgatgta tctcatcgct tgaagcgtag atgcaagaaa aaactgccgc aaaaagccct
    10921 ctgtatccaa gctttttcac ctttgaaaaa ttgatgaacg caagtattcc aagcaaggcg
    10981 tattcgctga aatgcgcaag ctttcttatg atgaacgtgg cggtgtcctt gtattccgcc
    11041 tgcgctgtag tgtcaaggct gtcatataca gggttgacaa attctatcac tgtgttaaac
    11101 accgcatttg attgcttgtt ggactcgtca gcattgtttg ccgaaaacat gaatatcacc
    11161 acgcaccaca gcacaagtgc cgacccttta agtattatct ttatcctgcc ttttgaagtt
    11221 gctttcaatg tagcctttca gctcctcttt tttgttttcc accttttcgt ctataacacc
    11281 attcaatgca agttccagcc caatgccgat atcctttccg ccaaagccca tttgtataag
    11341 atcctttccg tttatgtcaa ggtcgcaaat gtggatacac tcgttgtctt cttttatctc
    11401 cattgcaagc tctgcgatat ggtcaagttc tgcaagcttt tcctgccgtt tgtagtccga
    11461 ctgggcatat gtgtcagccc tgcgcacttc cagataatcc ataacaaagt caaagtcatg
    11521 ctgagccata aatctccgca ccgacttttt cgtggcaggt attctgttgt catgctccca
    11581 tataagctca taaatatatt ttgccgaagc gttgtctatc ctgagacttt ttaatatctt
    11641 ctccgccatg taagctccct cgaactggtg ctttttaaag tgcgaaatgc cgttctcgtc
    11701 aacagtgtgc attctcggct tgccgatatc gtgaagaagc atagtaagcc tcatctgcca
    11761 ctgtggtctg acgttctcaa cgctcaaaca aatgtgaccg taaacatcaa gaaaatggtg
    11821 gggattgttc tgtctgaaat caaagctttc ccgaagctca ggtatgaaca ccgctatgac
    11881 ctcacgatag tcaagcaata tcttccttgt accctttcca cagagaagct ttttaagctc
    11941 cgccgtaatg cgttcggcag aaatgctttc taaaagctca cgttgtccaa gtatagcctt
    12001 tgaggtcttt tcctcgatct caaacccaag gcatgaagaa aatctcagcc ctcttaatat
    12061 cctcaaagcg tcctcgtcaa aacgcttttc aggctcgcca acgcatttta taatgccctt
    12121 ttcaaggtcg ccttgtccac cgtaaaggtc aataagcccg tcttttggtg agtatgccat
    12181 agcgttcacg gtaaagtcac gacgttttaa gtcctcctcc agcgagcttt caaatgtcac
    12241 ctgagcaggc tttctatgcc ctatatactc tccgtcactg cgataacagg ttatctccac
    12301 cggctcgccc tctatcaaaa cagtgagcgt gccatgcttc agcccggttt caatagtgcg
    12361 gaaatttgcg aaaacttcaa gcattttgtc aggggtgcag tttgtgcaga catcgtaatc
    12421 atgaggcttt ttgccaagca aactatctct tacacagcca ccgactatat atccctcgta
    12481 gccgcactca ttcagcctgt caagagccgt cctgacaaac tgcggaataa agatctccaa
    12541 tttactgcct ccctttatag ttttatgaat tatgtgcgac tttcgggtca taatatttac
    12601 atgaatatct cgaaaataat tgaccccaaa atctcacgga gagcgttcct cgccctcacg
    12661 ggtatagtga gcgcacttat gcaatctatc ttgaatttgc cgtggcttgc tttttttata
    12721 tttgcgccaa tggtgcattg cctgtcagtc tgccatgaaa aaaaggcttt caggcttgat
    12781 tttctgtgct tttttgtacc atattatctt tttcagctca cttttatgct caccatatgg
    12841 aaaatatgtc ctctgccgtc ctttgcctct atacctcttg ctactttagc gtggttaggt
    12901 ctgaccttgt gggagtgtat ccttatgtat cttccactga gcctttttct gcccctgcga
    12961 ccgcatctca aacaccgttt gtctgagata gttttgctct gcctgctttt gacagcagga
    13021 gagtggcttc aagagaaagt tccgattttg gcgtttccct ggtctgcggc atggctagct
    13081 gtaacaagtt cgacactgct tttgcaatcc gccacgctca taagctgccg ccttacaagc
    13141 tttataattt tcctgctcaa cggtctgcat ggggagatat atactagcaa aaagcgtttt
    13201 gcctacaccg cctttgcact gcttttgcag ggggcaaatt taagctacgg actttattcc
    13261 ataaacctcg acaaaaagct tgataacatc taccctcaga tagatgttat ctgcgcacag
    13321 gatagtctgg agggcaggga aaaatcacac tgtaaggccc ttgactcagc acgctcctat
    13381 ctttctataa tggaaagctg ttggcggtgg ggtactgacc ttgttctgct tcctgaaact
    13441 gccgtgtcga aagactatga cgaacagcta aaagaatttt cccttatatc agactttgcc
    13501 gccacccatg ggtgtactct tgtgacaggc tcgttctatc ttgaaaacgg caaggattac
    13561 aacgcccttt tcgcctacga taaaaacggc attgcgtctt cgccctacct gaaacaagtg
    13621 cttgtgcctt tcggtgaaaa gacacctttt gcaggcatct tccaccttga cactatgagc
    13681 gaatgtgcag acgaaagaca caccaagccc ctcgcctcag acggcatgac cataggctct
    13741 gttatctgca tagagtccat tttcccaagc cttgtgagcc gacagaaaga gcagggcgca
    13801 cagataattt gcgtttccac caacgattca tggtttggca aaagctccgc ccgtgaacag
    13861 cattaccgcc actgcataat gcgagccgtt gagaacagaa gatatgtcct ccgtgcagga
    13921 aactgcggaa tatcagcgat aatatctccc acaggcgagc aactgtccgt aaaatcggac
    13981 aaatcaaaag gtgcggtgtg gggaaaagtt acccttattg gcaggtaact ttttttacca
    14041 ctctatctca aaaagtctag ccatataagg tctgtcaaac ttaacagaaa cattgcctgt
    14101 cgcctttgaa aactccacag gggtttcgct gaaatcagga taaatgcact tcacccttgc
    14161 gtcaacaccc ttgaggaagc ctataggcaa aacgcaggtg tcgctcttgc cctctctcct
    14221 ccatacggca agataagcct tgttgccgtt tctcagaccg agagataccc agtcgtccga
    14281 aaatttggac agccccaagc tccagaacgg cactgcttcc tttatgtccg cccttatctt
    14341 cttgtaaacg ctgatagctt ccttgacaag tgccttgcgc tcagggtcga tgttgccaag
    14401 gtgaccgctc tggtggatac gcagaagcat agcgttaatc atgttgaaaa caacttcctc
    14461 cctgtcacct gatgtgatag gataagccca gatagccgat tgctcagggc aaagagcagt
    14521 aggggagttc gccgcaatgg tgcagtattt tttgtaatcg tcctgatctg atgtggactg
    14581 aatggagtat cttgaaagca ttgcatagtc cattctaagt ccaccgctgg agcagttttc
    14641 tataataagg tcagggtgac gagcaaaaac accgtcaagc cattcaagat aagccttttc
    14701 atgaccccac agaccctcgc caacgctgtc gcagttctgc tcagtgccta taccaggctc
    14761 tatgttgtaa tccattttta tgtagcctat gccgtattca gaaacgagcc tgtctattac
    14821 ctcgtcagcg tgggcacgga ctttcggatt cctgaaatca agctgataac ggcttctgtc
    14881 atagactctc ttgccgtgac gtgtgaagaa ccaactgtca tcgcaaacgc tgtccacaag
    14941 cggacacttt attcccatga cctctatttc aagccaaagc ccaggtatca tgcccttttt
    15001 gcggatatag tcggcaactt cctttacgcc gttagggaaa cgctttctgc tctctttcca
    15061 ttcgccgacc tcgtcccacc aaaagccgtc agcataccag cctgcgtcaa tgcagaaata
    15121 ttcacagcct gcttctgccg cagcgtccac aaggggaaac tccttttctg ccgtagggtc
    15181 gccccacaga cagttcatgt agtcgttgaa tattaccgca agcctctgat tatccttgtt
    15241 gcggcgacgt atttttctgc ggtatttcgt cagctcgccc atagccttat caaagcttcc
    15301 tgtcaccgag cctactgcac atttcacgct ttctatgctc tcgttaggca caaggttttt
    15361 ataccagtgg gactctatct ccgacggccc agaaagctgt aaatagaagt gtcctcccac
    15421 gtccgaaagc tcccagtgcc aagagccgtt ttgctctatc tgccaaaaca gcaccgagcc
    15481 tgcttctgtg ttttcaagca cacccattgg tatgtattcc tttgccgacc agtttccaac
    15541 gtttgtgcag gcaaatacct ttgatgaacg ctgttccgta ggcttagcag atgaagcaag
    15601 ccccacctgt tcaagggtgt agtcctgcca ctgcaattca cgctgccatg agttgtggca
    15661 tacccatatc ttcatcttgt cctcaggctt tttaagcccc tctttttcta cgcccgtcag
    15721 cgcaaaggag gacatatatt caatggtctg tacctcgctg cccttgtttg taagggtagt
    15781 ccaacagcgt acaacagacg tgccggagta aaactgatag tgggacacag cttcaagacc
    15841 tgtgccctca tcaaatgtca caagctcaag cttcctgcca tggtcattgt tcacatcacg
    15901 gaagtccttg aacttcattc tccagccagg agcggtcaca atatatttgt ttccgtgacg
    15961 ctcgtcaggg cggtcgatac cgctcgcctg tatctctaca ggtctgaaag ttttcagccc
    16021 catgtatgaa gccgtcttgg cttcatcaaa aggcagagca gagaagtgca gaagctttgc
    16081 ctcgttctcg tctgttatct caaatactat attaatgccg ttttcatata tctcaatacg
    16141 tttcatatgg cgtatttccc ttcgtgatct atttatctgt acatattatc gtgcacttat
    16201 atcattttcg caagctcgtc aagtatcctg tgagcggctt gctctttggt cataataggc
    16261 agctccacag caccgccctc agtgataagg gtgataacat tagtgtccgt cttgaagcct
    16321 gcgccagcgg tctttagtga gtttgcgcat atcatatcgc agtttttgtt ggtgagcttt
    16381 ttctcagagt tcttgataac gtcctgcgtt tccatggaaa aaccgcatgt gacctgaccg
    16441 tcacgtttgt gctgacctag atagccaaga atatccttcg tgcgttttag ctctatcttc
    16501 atgtcatcgt cagacttctt tatcttgttg tcagccaccg caacaggcgt gtagtcagcc
    16561 acagccgccg cctttacgat aatatcctgc tcaggagcaa gctttgtgac agcttcaaac
    16621 atatccgccg cagactttac gttcacaacg tccacgaaca taggcggcaa aacctccatg
    16681 tgacctgcca ctaacgtcac ctttgcacct ctttgcatag ccgctttagc cactgcacag
    16741 cccatttttc cgcttgaatg attagtgatg tacctcacag ggtcgatagc ctccatggtc
    16801 gcaccagccg taacaagcac ctttttgcct gcaagatctt tctcgaaagc aagctcccga
    16861 agaatgtatt caaacaacac ctcaggctca ggcattctgc cgtcgccgtc atcaccattt
    16921 gcaagcatac ctctgtcagg agcgatcatc tcgtagccga aacgttcaag cttctggata
    16981 ttgtcctgca caataggatt gtgatacata ttgtgattca tggcagggga tatcattttc
    17041 ttgcaagggc aagccatgac agtggtggtg agcatatcgt cagcaatgcc gtatgctatc
    17101 tttccgataa cattagcgga agcaggagcg accataacaa tgtcagcctg ctttgcaaga
    17161 gcaacgtgtt caacactgta ttgaaaattt ctgtcaaaag tatctataag gcacttgttg
    17221 cctgtgaggg attcaaaagt gatagggttt ataaattgag tggcgttctg tgtcatgagt
    17281 acctgaacat tgcaaccctg ctttttgagc agtcttgcta aagttgggat cttgtaagcc
    17341 gcaatactgc cgctgacgcc gagaacgacc gtcttacctg aaagaagcat agagatgacc
    17401 tccttagtgg aaatatataa ggcaacaccg cacaaagccc gcctgacggc tttgcacgcc
    17461 atctgcttgc agattttccg tcatatcaca caaaaatctg actacgcatc tgacgcacaa
    17521 tctttgtgcg gtattgcctt aaatatagta taacacagat catattgaaa tgcaagattt
    17581 caagtgataa gtgagcatgg aaaataacag aaaaccgctg cccacagatg cccgtgaaca
    17641 gcggtctgaa catatgtcaa tccttgtatt gcgaagttat cccgaagtat tcttcaagag
    17701 aaagtccgct gttggttatg ttttgtgcta aaggcgtgcc tacatatctt atatgccaag
    17761 gctcgtactg ataaccggtg taagcctcct tgccatttgg ataacgaatt ataaaaccat
    17821 atttcgcaca gttgttggca agccacttgg cttctcttgt gccgccaaat gagtcgctgg
    17881 cgttgtttac gtctattgcc atgcccgtct gatggtcgct gtagccgggt cttgcggagt
    17941 atttgtctgc cgcagccttg ccgtcacggt tgcagtacat attataaagg tacctctgat
    18001 cgtaataaga gcggtagcct gagcataccc acaggcttat gccgtcttta tatgcagcgg
    18061 tctgcatctt tttgaatgcc gctgatgttt ctttggtcag accctttggg tcatagcttg
    18121 cagggaggga atatgttttg ttcgccaaaa gaactccgtc aacatagctc atgccgttta
    18181 tgacctcgac agtgtggtct gtggtcttga cggttatctc ctcggaatat gctccatagt
    18241 gatttgaacc tttatcgtcc gtcttgcagg ctcttacctt gaatctgtat gtagtctttg
    18301 cgtaaagtcc gtttattgcg caggaattta ccgtagatga agctatgttc ttccacttgc
    18361 cgtttgtgta tataaacacc ttgtagcttg tgcagcctac agacttccag ttaaggtcaa
    18421 gtgtagaagc ggtgatgtca gaagctttta ctcctgttac cttccttgga tttgtggcgg
    18481 aattgtagtt ttcacaatag tcgctgtata tcttaactcc gtctactatc ttatatgctc
    18541 ttaccttgaa attctccctc gttgccgcag gcagaccctt gacagtgtag gtcgtgcctt
    18601 tcacgtcagc tatctcagga tatttaccct ttgaacgcat tcccacagaa tagccgtccg
    18661 caccgctcac agcgttccag ctaagcttta tagagcttgt gttaggggtc ttagatgtca
    18721 gaccagtgac cttgttcacc ctgacagcct gtgccgttat ctcctgagaa tatttgccgt
    18781 agtgatttgc acccttatcg tcacgcttgc aggctcttac tttaaattta tagcttcctg
    18841 cgtcaagtcc tatgacagtg tagctgtttg tatccgtctg cgctatctgc ttccacttgc
    18901 cgttttccag cataaagaca cgatagtttg aacagcccac ctttttccat gaaaggctaa
    18961 cggaagcatt gcccgtctga gctttaagac cgcttatagg gcgtggattt gtggcggaat
    19021 tgtagttcac tgaatagtcg ccatagacct tttgaccgtt cactatctta taggctctta
    19081 ccttgaagtt ctcccttgtt gccgcaggca gacctgtaac aagatatgta gtg
//
LOCUS       DAWEMR010000028        18722 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1227_length_18722_cov_12.323673,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000028 DAWEMR010000000
VERSION     DAWEMR010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18722)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18722)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18722
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1227_length_18722_cov_12.323673"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5865 a         3786 c         4530 g         4541 t
ORIGIN      
        1 atacgggaat ataggaacta aaaattcgat taatgggaac tagttcccat taacttatca
       61 atgtcccact gtgcggctcg aacaatcact acagtcggaa gatcaagcac aagttgctta
      121 gtttcatatt tttatatcat acagttccaa taacctttcc ccggcaatag atactatcat
      181 aatcatgcag cggaatatca tcataatccg ggttaaggga aatcaagcga tctccgccga
      241 acttctttat aaatccttcc ccgttaagta taaaaatgcc tatctcgccg atctcaacag
      301 atggctgcga ggctatcaga actacgtctc cgtcgtggaa tacaggctcc atgctgttgc
      361 cagacactct tagagcaaaa ttagcctcaa gagtgagctg cgttttctta acctccaaca
      421 tacctttttc gcagccatcc agatcaacgc cagttccggc actgacaggc aacgaatagt
      481 actctatgaa aacactgttt tcaagctctt ttaatggtat ttttgactgc tcaaaaagga
      541 ccttggcacg ttcgaggact tgaccctttt tcattaaatc taattcctta taatatgtaa
      601 gtaactcttg ctcatcatcg gtcaactgtt ccgctggtga gcttttttct ttgccgtaaa
      661 caaggtaatc tattgaaaca tcaaaaaatt cagcaatttc aattagatat tttctatatg
      721 atttgctttt cccagacttc cattctgaaa aagcaacatt atttaatttc aggtaattag
      781 tcaattcttt ttgctctcga ttgcctaaaa gcatagttat tctatccaaa atatccaaaa
      841 ttcaatcatc tcctttgtta cttatcacga aattagcgtt tttctaattt cgagttagaa
      901 tttcacttga caattagaaa aaatctagtt ataatttact atagggtaac ctctagggca
      961 tagttacccc atgataaatt aagtttaaca caaaaagcgt taaacgtcaa tcgaaaaagg
     1021 aggaatttta atgaataagt ataagattaa ggaaaccgat gtgaatatgt atgagtacag
     1081 aggctatacg atatcaggta acgataggat aggctatgtt tgcatatcac cttatggggc
     1141 tttctcgcca aaggcttata atacaatcga ggatgctaag gcagcaatag atgatgatct
     1201 tgattgcata gctgaaaaat acggcgagca gcaaacaagg gcggaacaaa ctccgccaat
     1261 aagaggatat ataatgaata aagctttatt cgtagaaaaa gagctctatt tcctgctcca
     1321 tgccgccaat gatgatgttg atgatataac ttactgcgaa gacaacggag aagagtgggt
     1381 gcttgtaacc atgaagaacg gtcatgtgta tgatataggc atcacaggag acagtccgct
     1441 tgctgccgct aaagacgtta tcaccgttat gatgtataag taaggagggt ttattatgaa
     1501 aaaacttacg gacgagggaa aggtgcttac catgtttgcc attatcatgc tatggtgtgc
     1561 cataacaagt cttagcggac attccgagtt ctggatcata ccgttgctta cactatccgg
     1621 attgttctgt atgcacctta tcatctctct tatcagagat atatactttg ctaaaaggag
     1681 ggaacgcctt tatgagcgca gatattggga aaagcataaa gcagattaga gagcagcgtg
     1741 atcttaatca gagagagctt gctaagtatg cggggatcag caatgtgacc gtgtgcaaga
     1801 tagagcaggg cgttttgacg ccctctctta agactacgat caggatagcc aatgtgctgc
     1861 gatgcagtct tgacgatctg tgcggcagat cacgaaacag ccgaaacagc cgaaacagcc
     1921 gacagtaact cggctgtctg ccggggacgg tctcccggta ctgatgatgg cagaccaaaa
     1981 tatgcaggtg ccgtgcttgc ccgccatagg ggatatagtg aatcacctgc agcttgcaag
     2041 ctcagaggta acgacttaga tcttgaacca agcctgtcat acagtctgta gaccgaggac
     2101 ataaaatatc ggtagcgtat gacttaaaaa ccacgtgttc tggctacgtg tcgtcgggtt
     2161 gaattagccg aggtacaggt tttgttataa gagttccgac tttccttaag gtgcgaactc
     2221 gtaaatgcaa gctgagagcg catgagctta atgtttgcac cagctggaac gtcttttgta
     2281 tttttataac acagtctaaa gacaacataa agataccgcc cgtctgagcg tatcagaccc
     2341 acttaggata actttttatc ctatctacct tgccgtttcg gtaataatcg gggcggctaa
     2401 atgttcacgc atagtgctag gttcgcaagc gtaagcctat caggtgcaag tcctgatgtg
     2461 aaagccaact caatcaacct agtcgacaag gttgtgcaac gcagtataag tgcgcagggt
     2521 actctttcgg aaggagtaga cttatacatt ccgatttttc tagccgaaac cgagccgcaa
     2581 caggcggttc ggtctgccgg ggacggtctc ccggtactga tgatggcaga ccgagaaagg
     2641 aagtgtaacc aatgaaatac aaggaattta aaacacaagt actgtctcat gcggtgggac
     2701 aagcccggct tctggctcag gcaagctgcc ttgaggacat aaaaatagag cctgaacagc
     2761 tccgcaggaa tgttgcgacc attcttgcag tattgaccga aacagaagat tttctgtccg
     2821 acctcgtaaa caaccgttct ggctctgata cttagtatat cagaaaacat tagttttgtc
     2881 aagtaacttc gggaggtgat gatgtggaat atttgacggt tgccaaaact gctgatctta
     2941 aaggctgcag tgaacgatat ataaagaaac tgtgcaagga cggaaaattg tcatgcaata
     3001 tcgaaatcaa cgacaggaac cgtcctaaat atatgatccc aatatcatca cttcccgaag
     3061 gcctgcaggc aaaatactat gctcagaaaa gagcggaagc gggcttagaa cttgttgcac
     3121 agcctgttaa aaatgcgtta aaacagcccc aaaagcctgt gaaaacgtca aagcctgtga
     3181 aaacgtcaat cgaagagttt tccgaagatg agcgtgatga aatcgcactc tgggtagata
     3241 tcctcagaga ctggcagcgt taccgggatc aataccccgg aaagaaaacc gaagtggata
     3301 agctttacgt cggcaagtgt cagctcgagc ataatgatat caaagtgtcg gtggatatac
     3361 tataccgcaa gtatgcagcc tacagaaata ataatctgca ggggctttgt gaaaatcgag
     3421 gcggagccaa taagggtaag agcagtatcc caccggagct gtgggagcag ttttgctatt
     3481 tctatctttc cgaaaacaaa cctaccgttt cgcgttgtta cgacctaacg cttgaatgtg
     3541 caaaagagtg gtatccgtca atggtatcaa acttcccgtc agacaatact ttcaggcggc
     3601 atataaagtc ggaaatacca caagccgtgc ttacatatat gcgcgacggc gataaggcta
     3661 tgaaagataa atgcctgcca tatatcagcc gtatgtacga cggtctccac gctaacgacg
     3721 tctggatcgc agataaccac acgttcgata tacagtccta cgatgaagat aacggcacga
     3781 tccacaggct gtaccttaca gcctttttgg atgccaaaag cggagtgctg gtcggctgga
     3841 atatatgcga cagtccgaac tcccagtcaa caatcatcgc attaaggcat ggaattatgc
     3901 ggtttggcat tccgaaagcc gtatattttg ataacggtcg agagtttttg acccatgacg
     3961 ttggcggaaa aggtcatcga agcaggaaaa ccgataaccc tgagatcgaa ccaccgacaa
     4021 tactccaaag gcttggaatc acgatgcata acgcaatcgt ccgtaacgct aaagcaaagc
     4081 ctattgagcg tacatttagc acggtcacaa tgcagtttgc aagaatgttt gaaggttact
     4141 gcggcggcac tattatgcag cgacctgaga gccttaagcg tagaattaaa gaaggcaaga
     4201 ttccctgcga ctttgagatt agagaatata ttgatatgta tattgacggt gattttaata
     4261 tgcaggaata tggcggagct gagacaaagt acaaaggaat gagtcgtatt gacgtatgga
     4321 atatggatat aaagtctgta ggaatacgca aagctccaga agccgaactt aatcttatgc
     4381 ttatgagatc aacaagggtg caaaagatca agcgcaacgg cgtatttgtt gaaatatccg
     4441 gcgaaaaggt ctggtttatg gactatgaaa acacttaccg tcacctgggc gaggaggtct
     4501 atgtaagata cgatcccgcc gatcttagaa gcgtaagggt ttacgataaa tcggaccgtt
     4561 acctttggac ttgggaatgt gcggacaagc tgcttataga ttacatcacc gaaagcaagg
     4621 aagagatttc cgatgcaatg gctttacagc gcagagtaca acggtttatt aaagccgagg
     4681 ctcagaatat cacagatggc ttaaactcag agcataagat cgaccttatg gaggccgccg
     4741 ctctcaaggc aacacatggt aagcagagct ttaagatcgt tatgccgtcc aatgtgatta
     4801 tgataagagc tgataatgaa ccggaaagta tatcaaaggc ttccggagac gatattgtgg
     4861 tcaatataga taaaatgaac gaaaacgccg agaggcggaa gaataaatgg aggagtgatt
     4921 aataaatgaa aaagctaaca gctaaacagg agtgggcatt ggaacagata aaacagctgc
     4981 agtactcgga aaatctatct gcggctgcag tctgcaaaaa gattggtata tccgatagct
     5041 catactctgc aattaagtca ggtacctaca atggcgatgt agataagcag atgaaaaaag
     5101 taattgaata ctttgaaacc aagcgggctg cagctgaaat ctatgtcggc acagactata
     5161 aggaaacgtc aatatcgtct aacgtgtaca agatcatacg caactgtcag cttcagggcg
     5221 gtcttgccat agcctgcggt gacgcaggta taggcaaaac acaggcttgc aggcagtatt
     5281 accgtgagca cggcacaaac tgtacataca taacggtaaa tccgtgcatc aagtcatcaa
     5341 aatccgtgct ggaacttatc ggttctaagc tgaacgtatc ctccggctct gtgagcagac
     5401 tctggttgga aatctcgtca aagctttcgg acggcatggt gataatcgtg gacgaagccc
     5461 agcatctcac cagaaatgcc attgatactc tccgaagcct ttgcgactgc tttgacgaaa
     5521 agggacagac ccttggaatc tgctttgtcg gcaacgagac cacggtaagc agacttggcg
     5581 gaaagcagaa agcggagttt gcacagatac gcaacaggac gaagaatacc cggttttaca
     5641 gcgttaagca gataaagaag agcgacatcg aaatgctctt tccggatatc agagaggacg
     5701 ttgctgctgt tgaattttta ctgcgtatcg ctcaaagtcc acaggctatc agaggagcgg
     5761 tcaatctata ctccaacgcc ctcgataacg gcaatgtgac cgccaaggga ttgtctgcaa
     5821 tcgctaaata tatggatatg gcggtataaa aagtgaaacg gaggacaaaa agaatgaagc
     5881 acggaaaaaa tcccaccaaa gctcagaaac gaataatagc gtattacaag cttgatcctg
     5941 cggactggat ggtttcgaag gcgacggaca agcagctttg ccttgtacat agatatactg
     6001 ataagatacg ctggatagac atggtacgca tcgaggagcc gaggaaagta aaggcgacta
     6061 aatatgcata ataattttga atgttttatg aaaggcagtg cagaatgtgc cttttttatg
     6121 aaaatgtgtt gtgatgactg tccgtactgc gataaatgcg gctattgtga taatatttgc
     6181 aacagtaacg gagaatgcaa tgaatgctca attgtagaca agaaataata agtatcagag
     6241 gggctgtgcc cctcctgtaa tgcagccgcc gattggcgca ggtcacaagc cctgataaat
     6301 gcagagtgca gaaaaacaag gaggtaaaaa cgctatggaa acaaaacata aaaagcttac
     6361 aagcaaagct ggattgacga tcccgaagga catcagactt gcagcgggct ttgcgggagg
     6421 catggctgtt gacatcgaga agacagcgga cggtatcatg attcgcaagc acagacctac
     6481 ctgttgctat tgtggcagtg ttgataatgt gcgctctatc aagggacgtg atacctgcag
     6541 aaactgcgcc gaagaaatca tagaggaggt aaagacggct tatggatctg tctgaaaagg
     6601 taaaacgtta tgctgagatc aaggcggaaa tttcggagct taaatcagag gcagacggca
     6661 tcgaggctga tatccttaag gcttcggaag ccgacctgca ggatacaaag tttaaatctg
     6721 ctgtctacag cgacaatgct ggcaatgcaa tcacagtcac caacgccgac aacgttaagc
     6781 ttgtgtaccc cacaatgctt aaggagatct ttggtaaagc gtacggcgac gttgtaaaag
     6841 aggacgtaac ctacaccttg tcagaatctg caaaacgctt gctttccgcc gtttacaaca
     6901 aggagtacat aaaggacggc agcgttgcta agatactgga cgggcttggg cttgacgata
     6961 agagccgcaa agttctggag aaaaagctta agggtgcgaa atatgagacc gacgtaaaaa
     7021 atcttatgca gcttggcggt ctggatgaaa aggcggcgca ggagaacgct tatctggtat
     7081 ccgaagccgt cgcttggcag aatcttaaac gtctgcttat gattaataac gaacagctta
     7141 ccgatgagat tgtggagcgt gctgtggata tgattgacag cgcggttgta gttgaaagaa
     7201 cgccgaaaac gaaattcacg gctaaaaaat aaggacagga ggatttggat atggcaacaa
     7261 aggagcagat taaaagaatt tacggtctgg gagcaggtct tggtattgtc ggcaaagata
     7321 aagatgatat gctgcacgaa ttgatcttta gcattaccgg taaagattcg gtaaaacagc
     7381 ttgacgatag cgaattcaag gctgttcagg cggaacttat caatcgcatg aagcttgccg
     7441 atccaaacca tctgctgcat aataccaaat ctagaaacaa aaagaaagaa gctgaagaga
     7501 tcggctgcaa cggtatggct acgcctgaac agcagcggct gtgctggaga tactgctaca
     7561 ggctcaagga acttgacact aatccagagt cagctgacgt tggagacagg ctaattggcg
     7621 tgataggcaa agtactgggc gttacggcat caaaaaagca gccgtttcgg tggatagatc
     7681 aggaacagtg ttctaagctt atcgaacagc tcaagcgtta tgttaattcg gcagagcggc
     7741 gggcgaaaag gcaaggtgag aaatatgccg gaacttgata tatacgaaga agaccttact
     7801 ccagagcagc gggatattta cgactgcatc ggctcacagg catacgaaaa gcttgtgcag
     7861 cgttacggtg gtttgtcaat ttacattgca aaagctgatt ctgttatccg atcggcacgt
     7921 gacgaaaaga tacgcaggga ttttaacgga tataacttca agtttcttgt caataaatat
     7981 aatctgtctg agcgcacgat ccgcagcata acggctgaga taaggcagga aaagcaaaac
     8041 gctcctatcg agggtcagat tacctttgat gaaatataat tgcagaaact cgctgaaacg
     8101 cttcatctgt accaccccca atatatatgg tacagttatt ataacggata acggtactaa
     8161 atatattggg ggtgttttta tgacaagtca gcagatattt acgatagtat ttcagctcgt
     8221 tcttacaggc ggtataggta ttataaccta ctttttaaag cggactatgg acgacatcga
     8281 taaatgcaaa agcggtctgg ataaggtcag agaaaattac gtctctaaag acgagtttga
     8341 caagtgcaag accgatatta ccgacgtcaa gcagaactat cttaccaaag aggattttta
     8401 cagagaacag cttaagactg aacagaagct ggacaagatc atggatatct tgatggaaat
     8461 gaagggagaa aaatagcatg gatatggaaa agcagatgca gcttatcaga gcaggcaatt
     8521 ttaaagaaaa taacggctct gttatgcgca ctataaatat gctcagatat cagtatcata
     8581 agcttaagag cgttgagtat gctcttcccg atataacaaa gggcgaaatt accgacagcg
     8641 tgaactatct ttatgaagcc ggatacatac atctgaggac ggtgttgtcc aaagagccgt
     8701 ctacgctggc agacagcgat tttgacgatc tcgaggcaaa gcttacggca aaggggatca
     8761 gcctgcttgc cggaggtatc aacgacccct gcataaagct gtagggggtg cggataatgg
     8821 caagaaagcg cagaaagcac tctaaaatag acaagctgtc gccggaactt aaagcaacgg
     8881 tcgaagatat gatgaaagcc gattttacat atgcggagat cgcagactat ataaaggatc
     8941 agaccgatca gcccatatca atatcctcgg tttgcagata cgccgcaaat ctgaatgaat
     9001 ctgttgagac cctcagaatg gctcaggaga atttcagggt cataatggag gagataaaca
     9061 agtatccggc tctcgatacc agcgagggaa taatcaggct gctgtcgcat aacgtgttgg
     9121 aatctataca gaacactccc gaagaaaaat ggaagaacat agacccggaa gccttgctca
     9181 aacaggctac cagccttgta aaggctgcgg cgtataaaaa gaatatggat ctgaaaaacg
     9241 aggatatcct taacgcgggc tttgaacagg tcaagtcaat ggtgttcgag gcaatggcaa
     9301 gggaacgtcc cgatctttac aaggatgtgg ctaagttcct tgaggagaaa aggagcgata
     9361 tatgatctac gttatttatg ttcagagcgg cagagagcat gacgttgttg ccgctctcag
     9421 agataaaaat attaacgcct atgcgcctgc tcacgacctg ttggaacgta aaggcggcgt
     9481 gtggcgcatg gtacgccgga tgatatttcc cacatatgtt tttgttaaca gtgaaggcat
     9541 cacagacgag ctttactaca ccgtgaaaaa tactgtcggc gtattgagat ttttgggcag
     9601 accgcccact ccgctgccga tgagcgagga agtcagactt cggtggatac ttgatgtcga
     9661 aaatcttacc gtcagccgtg gttacataaa cagcggaaag gtaactatca cagagggact
     9721 gctcaaaggc agagaacact gcattgttaa atacagcagg cggcgtaaac gctgtacgct
     9781 gtactgtgag ataaacggca ggcgtcatta ctttgacgtt gccgcagaac tggaaaagat
     9841 ctgatcataa gcgtaaggtt gattcgtccc ctgcgcttaa gctcagatta catagcaccg
     9901 gacatcaacg gaattttgaa acaaaaatat ccgaatggcg aagcattgct gtttgatttc
     9961 attttagcgg cgtttaacgg cgttatagca cgttttgaaa taattcttag gataatttca
    10021 cacttgaaag agaaagccct taaaacgggc gtgtagtttg agtttgagcg aacggaggtg
    10081 ttaatgtgaa tgtaaagaga aaacaggcta tcgacacgct gtcggcagct gtaagcaaca
    10141 ttaatgatgt aaaaatacag acggatatac aaagcctcgg cgagctgtcg gaggctttta
    10201 ttaatacctc cgacaaggca gaacggaaaa aacttgccac agattataaa aagcggcaca
    10261 aggaacttca ggattttttg gacgacaatc ctgaacttgt aaactccgaa gtggagagag
    10321 ctttgcttgc ggcggctctg ggcggcgaat atgcagagga agaagttaga gttgacgcta
    10381 gagggcgcaa aacgatcagg cgcagggtaa aaaaagtcgc tcccaatccg tccgccgctc
    10441 tgagctattt gcagaataaa gacaaagaaa actggtcacc gaatcccaag gctgatcctg
    10501 agctggagga cacgtcggaa attgaggagg atatctatgg caaggacaac taagcctgag
    10561 aaacgcaaaa agaccatacc ctacaatttt ggcgataagc ataaggcata tatccgaaag
    10621 tcacaggact gcatgataaa cgttgccgag ggagcggtaa gagccggaaa gacagtggac
    10681 aacgtccttg ctttttgtca cgagcttaag actactaaag acaagataca tcttgcatcg
    10741 gcgtcaacac tcggcaatgc gaaaatcatt cttggcgact gtaacggctt tggtattgag
    10801 catttctttc gaggtcaatg ccgctggggt aagtacaagg gcaatgaggc tcttatcata
    10861 aagggcaagg atacaggatt taaaacaagg atcgtcatct tttccggcgc tatgcttgcc
    10921 agcagttata agtccatacg aggcaactct tatggtatgt ggataggtac tgagatcaat
    10981 ctgcatcaca aatcatttgt gcaggaggct tttaacagat ctatcgccgc agataagcgt
    11041 aagatatggt gggatcttaa cccggacaat ccaaaaagct ggatatacac cgagtacatt
    11101 gacaagtacc agcaggacgc cgccgatcgc aaattcctcg gcggatacaa ctacgcacat
    11161 tttactattg acgataacat aaatatctca gatcagcgta aggctgaggt aaaatctcag
    11221 tacgatccga catctatctg gtacaagcga gatatactgg ggttaaggat agcggcagag
    11281 ggtcttatct tccagagctt tgccaacgac cccgaaaagt atataatacc cgaatcacag
    11341 cttgacaaaa gcaagatcac atcaatacag ataggtatcg acttcggcgg caacaagtca
    11401 aagaccacat tcgtggctac ggcttttatt gagggcttta aaaagcttgt cgttattgca
    11461 gatcacaaaa tagacggcgg caagggcgag gtcggtcccg atactattta cactgctttt
    11521 ataaagtttg taaagacgtt atatatgcgt tttaatccgc ttttaattaa atttgcatgg
    11581 gcggacaacg aaaaccaagc ggtaataaac ggtctgagag tagcctgtgc cagagcaaga
    11641 ctgatggtca agatcgtgga ctgctacaaa gctccacgaa acgacagaat atctatgctt
    11701 acgtctttga tggctcaggg cagattttgg gtgcttgaca tttgcaaaaa tgttatcgga
    11761 agcttgtcgg agcagatatg ggatcctaaa attccggaca gagacgagcg tcttgacgac
    11821 ggtacttgcg atatagatac cgccgacgct ctggagtaca gctttagcaa atttatcaag
    11881 ccgctaacgc tggcaggagg tgaaaacatt tgaacagtga gataataaac tggctgaata
    11941 ataacttcgg ctataacatt tcgaccgact attataataa tatatccgta tggaaagact
    12001 ggtggaaggg ttttcatgaa ccatttcata ggataacttt tgaaaacgga gaaaaacgca
    12061 agagtcgtga tatgtatacc atgaaaatgg ccaaaaaggt gtgcgaggac tgggcaagca
    12121 tattaataaa cgacaaaacg tttgtaaaag tagatgatga atactcggaa aagttcatcg
    12181 ttggcgatac cgacaacggc ggagtgttcg gcagcaacaa cttctgggat caggctaacg
    12241 accttatgga aaaaatgatg tattccggca cttgtgccgt tgtgatacgt cttaaaaatg
    12301 ctgtggtaag ctcagacggc agacttctgc cgtcaccgga cgcatggata gatctaaatt
    12361 accttgaggc ggataggata atagtcctat catcggacaa cggcattatc accgaagcag
    12421 cgttttgctc cgatatctgt acaaaaggca gcaacaagct gtatcttgag atacaccgtc
    12481 tggaaaaagg cgaatatgtc atagaaaatc acatctttgg aataaaagat aaatcgctgt
    12541 tgagcgaaga acctctgccg gacggtgttg caagaataat gcatacagga tcagacaagc
    12601 cttggtttac catatgcaaa cctgctatcg ttaatcccat taacggcaat aatgggatgg
    12661 gctgtgcggt ttttgccgga gcgattgaca acttgaaggg agttgatctt gcatataata
    12721 atcttaactc tgatttttgg ttgggacaga aaaaagtgtt tttaaacaaa aatatgcttg
    12781 aagatatgtc tggagataaa aaggttgctc ccgatgaggt aaatcaacaa ctgttttatt
    12841 atatcggcga gactatggac gatggcacgg gtaagagtat ggtgcaggag cataatcccg
    12901 atctgagagt tgcagacaat acggcgggta tacaggcaca gcttgattat ctcagcttta
    12961 aggtgggatt tggtactaag cattatcagt ttaatgcagg ctctatagta actgctaccc
    13021 agtacacagg cgacaagcag gacttgatcc aaaacgcaca caagcatttt ataaaagttg
    13081 agagcttttt gcatggtctt gttaaaacgc tcctctggat aggccacagc tttatcgacg
    13141 cacaggtcaa ggaggacgca catatatcca tagtctttga ccaaagtcca ctggtagacg
    13201 aaaatgccga gcgacaacgt gacaaagatg atgtaacagc aggtttaatg cagaagtggg
    13261 aataccgtgt taaatggtac ggcgaatcgg aagaggaggc aaaggcacgt cttgcagacg
    13321 gtgaacctac cgacgatgag cttatgggct ttgaggacgg tgaggagtaa tgcttacccc
    13381 tcagacgtta caaaaactgc cagatgactt gattgatctt gtaagcgagg tacagactga
    13441 tataatcaag tctattgcca aaaagcttgt taaagcggac tatcttactc cctcggcgga
    13501 atggcagttg tacaaagcaa gccagctgaa gatgtctaca aaagagatca ctgctatgct
    13561 tgcagaattt acaggcaaat caaagcggca gatatcaaag ctgtacaccg atgcctgtaa
    13621 ggaggcaatc aacaacgacg ccaagatata cagaacttac ggcaaggact gctccgccgc
    13681 tctgaggtcg gtggcattat ccaacacgct taaggcaggc gttaaaaatg caaatggtat
    13741 gacaaaaaat ctgtgtaagt ccatggtaga gtcctcgcag gcaactgtta ctcatcttat
    13801 ggacaaggca tggttaaagg tacaaagcgg tgcttttaca tatcaggatg ctatttacga
    13861 cgcagttgtc gagcttgcta aacaaggtat tgcgactgta acttatccat cgggtaagac
    13921 cgactgggca gacgttgcag tgcggcgtgc ggtaatgacg ggcataagtc agactgcagg
    13981 tcagatgcag cttgatcttg ccgcagaaat ggactgcgat ctggttgagg tcaccgcaca
    14041 catgggcgcg cgtccctcac acgctttatg gcaaggcaag gtttacagca tttcgggcaa
    14101 atctaaaaaa taccctaaac tcagcaccgc cacaggctac ggaacgggtg acggcttgaa
    14161 aggctggaac tgccgacatg atttttatcc gtttttcgag ggaatttccg aacgtgctaa
    14221 tctccctgtt gacgtgaccg aaaacaacag acagtatgaa ttatcgcaga aacagcgtgc
    14281 tatggagcgg tctatacgag ctacaaaaag acgtttagct gcatatgacg gtgctatctc
    14341 tgagacggaa gacgaggtgc ttaaacggag actgcaaaat cagtttgaac gccactcggc
    14401 tatactgaaa actaaggaaa aacggctgtc tgagttttgc gatacgaacg atctttatcc
    14461 cgaaaaggac agagttcggg ttgttggatt taacaagagt gtttcgcaga aagcggtata
    14521 tgggaataat cgttactttg ttaagcaaat gaaaagctac ggcatagaaa atccgccgaa
    14581 aagccttgac atttttgaaa atatgaagta taatagctct cctgagtaca aattgatgaa
    14641 tgcatatatt acttctgtaa aaaagggaaa aatttctccg cttgtaggtt acgatcatta
    14701 taagaagttg cacaatgaga ttaacaacag tcttgttggt ttaactacaa ctaacggtat
    14761 tgaaataact gggcaatcgg atcatttcat tgaacgtgtt attggtgtaa tcaaagatcc
    14821 tgatacgggt aagaaacgtc ttggcgttga acttcaagat attcaggatg ctttgaccaa
    14881 tggtaaagca atgaaaccca aaattagcag ggataaaaac ggtaacattt tatatgatga
    14941 agatggtaaa cctaaaatat ctcagctatt tgttacagat aaatgtgcag tatccataaa
    15001 tcctgaaacg ggcgtgctta ttcaatgtaa tccaaagtga ggtgtattat tatgatattc
    15061 aatttcaaaa aagatgaata tgaaatgctt gtcaaatatg gcgattttga agatttggaa
    15121 tacccataca agctcttccc cgaaacaagt cagatagaaa taaataataa agatgtttct
    15181 atgtttcaat gcataattag caacatatca gttgtttatg gcatggacga aaatcagaat
    15241 aatatgacag atttcggata taaagcattg gatatttatg ataaggttta ttttcaaatt
    15301 cataatgaat aagctcccag ctactggggg cttttaattt tgcaaaaagg agctgataat
    15361 ttatgctttc aacccttata ttgctttacg ccctcgatac agggcaaatc cccgttggct
    15421 gttatgtagc cgcatgggtc ttcacaataa taaaaggtat ttgtttggtt attaaatgcc
    15481 ttgtagattt atcagatgat taaaactgca ttttaacgat agtaaaacgc tctttcaagg
    15541 gcgttttatt tatcctcgtt tttgcggagc aaaaatccga gccgcttgcg aggagataaa
    15601 ttttatactc aaaattaagg aggaaaaatc cctatggaac tgaaagattt aacagctctc
    15661 ggtattaccg aggagcaggc aaacaaggtg cttgaacagc acacagcgga gctgaccgca
    15721 gaacagcaga agtacacaga ccttaacgca gaactggaaa cggcaaaagg cacgatctca
    15781 gagcttactg ataaggtcaa agcatttgac ggcgaggaca ttgaaggact taagaaagcg
    15841 gcgtccgact gggaaagcaa gtacaatgca gatattgccg cacttaagct tgacaaggct
    15901 ctggaactgt cccttgccgg agcaaaagca agagatgtgg acattgtcaa gtctcagctt
    15961 gactcgtcgc tcctcaagct tgacgatgac ggcaagatca caggtcttac cgaacagctt
    16021 gacaagctta aggccgacaa agcatttctc tttgcggatg gtgatgagcc taccgcaagg
    16081 atagacaccg gtcttgacca cggttcggca acagaaacaa catcagacgc acaggcaaga
    16141 gccgtaatgg gtctgcctgc gactaagtaa ttttacggag gtaaaatatt atggcaaacg
    16201 caattacaaa atttaaaacc tacatcgcac tgcttgacga ggtttacaag caggcatctt
    16261 tgaccgccga tctcgacagc gatcctactc ttgttaaagc gggcgcaaac gcaaatgaga
    16321 tcataatccc taagatctct atggacggtc tggcggacta ctccagaaac agtggctatg
    16381 taaagggcga cgttactctt acaaacgaga ctgttacctt taattacgac agaggtcgta
    16441 aattcagcgt tgacaatatg gacaatgagg agactgcagg acttgctttc ggcagactgt
    16501 cctcagagtt tatccgcgtc aaggttgctc ccgaacagga cgcattcagg ttcgcgacct
    16561 atgcaggtac aacaggcata tctaaggttt ctgcaggagc aacactttca tcgggtaatg
    16621 atgtccttac cgctctgatc actgcacaga acaaaatgga cgaagacgaa gtatcgccgg
    16681 aaaaccgtat tctgtacatc actcctactc tgtataacct tgctatcaat gtggacacca
    16741 caaagtcaaa ggctgtactt gacggctttg ctaagatcgt aaaagtgcct cagagcagat
    16801 tttacactgc gatcgatctt aaggacggca cgacaaaatc agagggcgtc gatgaaacgg
    16861 caggcggttt tgctaaggcg acaaccgcaa aggatataaa ctttatgata atccagaagt
    16921 cggcggttat ccagtatcct aagcacacgg taaacaaggt cgttacgccg gaggaaaatc
    16981 agacggatga cagctggctg ttcttcttcc gtgcttatgg tctggctgat gtgtacgaaa
    17041 acaaggcggc aggtatttat ctgcaccaca aggcatagga ggtgtcgtta tggcaaagac
    17101 agtaggattg acttttaacg agaagccaat aacaatagaa ccgcctgccg actattgcga
    17161 aaatgaaaat gtcattgatt ataagagcat gacagtccct gaactaaaag cttatgccgc
    17221 cgaacttggc attgacctcg ggtcggcaag caagaaggac gcaatcattc aggaaatcgt
    17281 cgatgcagtt gcggtagagg atacgacaag cgaaacggag gtgtaggcta tggcttatgc
    17341 tgattacacg ttttacactg ctgattttca tggcaataag atttccgaaa cggattatcc
    17401 ttattttgcg gagcgtgcat cggaatatct cgacagtctg agctttgccg agaccgacga
    17461 gatcagcctt gccaaagcct gctgtgcttg tgcagagatc atgtactccg cacagccgga
    17521 taaacagata gcctccgaaa aggtcggaga ttattcgata agttattcga ccactcagac
    17581 tgctgtcgcc gatgagctga tcaagaccgc ctccagatac cttaatctca ggtctgtggg
    17641 gtggatataa atgagatata atacaaaatg caccgtctgg cacaaacagc ccgacggtgc
    17701 atttattaca cagcattatc cgtgctggtg gcaggacacc gaagccgaaa acattgcaaa
    17761 gacgggcaag accgatgttg accgggcgtt gatacatctg ccgttgttgg ctgtagtcga
    17821 taagtccgac tatattgcaa agggcgatat tgattttgac gtgacagcct ctgtggcaga
    17881 gttgcttaag gctgtaagcc cgctcaaaat cagtactgta gagcgcaagg attacggcag
    17941 tcccataatg tggcacacgg aggtgacggc taaatgagta ataacagtat taaagtaact
    18001 ctcacggtcg ctcccgaaaa tgaactgttt gcgaggcgtg gtctgcaaaa aggcggccga
    18061 gtacagaagt atatcgacag cgaagtatta cgttgctgtg atagttatgt gccaatgctt
    18121 accggtaagc ttaaacagtc aggtattaca tcaacggtag ttggatcagg catggtgcat
    18181 tacaacacac catacgcccg caaaaattat tacgataaca aaggtatggg caagcagggg
    18241 cttaaccttg ggggcaagcg aggcagacta tggttcgagc gtatgaaacc cgatcatctg
    18301 tccggaataa tcaaaggagt gaaacgaatt gccggagcaa aataaaagcc ttttggaggc
    18361 tatgaaagaa tatgtgttac agtatcccaa tcttggagat attgatctgc acatagacca
    18421 gactgagtct gaacctgtta attacagtat acagacatca ggtcttgtaa aactcagtga
    18481 ggacgtgtgc ggaaatcaga tatggcagta caatgctttg ctccagagca gagagtacac
    18541 agccgatgat ctgtcaaggc tcaatgcatc agcttttacg gaggatttta tcttctggat
    18601 tgaaaaacag aacagtagcc ggaattatcc agaacttgca ggaaattttg aacctatcag
    18661 tatatccgct gataatggca tactgcttgc tcaggatgag gacggagaca gaggattgta
    18721 tc
//
LOCUS       DAWEMR010000029        18321 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1258_length_18321_cov_11.321034,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000029 DAWEMR010000000
VERSION     DAWEMR010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18321)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18321)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18321
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1258_length_18321_cov_11.321034"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    14626..14635
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15631..15640
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4839 a         4031 c         3732 g         5699 t
ORIGIN      
        1 gacagggctt tgccctgtac ccacaatggg tttcacccct tgaccctgac cagggacgtg
       61 tccctggacc ttttgctcgc ttcgctcggt gttgggtact ctactttgtg cggcactgtc
      121 attcctcact cctcactcct cacttaactt cgctcctcat ttagcctcgc ccctgattta
      181 ttcaaatgta atcgttttat gcctattgca ttaattcgct ttttgttgta taatatattt
      241 gttaatatat acagccattt ggcaaaatca agataagaaa ggaccttatt actatgaaga
      301 aattccttgc ggcggctctt gctctttgca tgacaagtgc tgctctcaca agctgcggtg
      361 attcaaacag ctcttctgct gctgactcaa actccgctgc ggcaaactct caggcttccg
      421 attcaagtgc ggctgatacc gaaagtgttt cagacaagct tcttgctgaa tatcctgcat
      481 ctacggaaaa gatagatgtt aaaaacggtg ctactgagaa catgatcgcc agaagcgtta
      541 tcaacgaggg cgacacttcc cgtcttgcgg ctaagctcga ctacgctctg caaaatccta
      601 aggagacaac aaagatctgt ttcatcggcg actctatcac ggcaggttcg ggtgctaact
      661 cctcaactaa tcagtatgta aaccagttca agagctggtg ggaggaaaac ataagcttct
      721 atgttgatgt tactaatgcc ggtatcggtg caactgactc atacataggt gttcacaggg
      781 cacagcgtga cgctttggag gctaagcctg acattatcgt tatcgagttt atcaatgacg
      841 ctgatgatga attttatgag tcctgcatgg acagccttgt aagaatgtgt cttgagcagg
      901 acaacaatcc tgcagttatg atacttgagc cttcaactga gggtggtaca tctccacagg
      961 ctgcacatct caaggtagca caggcttaca acattcctat gatttcttat catgacgctg
     1021 ttatgcctga gatagaggcc ggcaacttca catgggctga catttcacct gacaacgttc
     1081 accctaatga tgacggacac gttataatgg cttcactgct cacaaaattc gttggcaaca
     1141 taaaggacaa tattaacagc gttgacaagg aagcgaaggc atttgacaca agcactgctg
     1201 caccgacggg cgatgtattc gctaacgcta ctatcggaag ccgtcagaca gaggacattg
     1261 taaagacaac tgacgaggga acattcacag atgttacaac attccagaag ttcacagacg
     1321 gttggggaac gacaacaggc ggcactatca agtttgagat aactgcaaag aacattggta
     1381 tgatatacta catgacagtt gacggcaagt ccggcgtggc tgctgtaaag gtcgatggcg
     1441 aggacgttgc gactatcaac gctgacttca caagcggctg gggcaactac gcaaaggcac
     1501 agcagatata ctcatctgac gaggtggcta ctcacactgt tgaggtaact gtcgttgatg
     1561 actcaaagcc taacttccag atacttaact ggcttatttc ataagagttt aaaagctata
     1621 ttttcggcgg atacatggtg ttgtatccgc cgaagttttg ttttcattta tgttatatga
     1681 attaaatagg acacaaaata tttagtgaaa actaagcagc cagcttaaca aaaacgaaaa
     1741 tagatatgat ataataaata tgctgaaaga aacaaacaca gcgataactt attgatcccc
     1801 ttaataagac ttctcccgac aggttttccg actgtcggga gtttttttgc atattagttt
     1861 tctttttgtt ctgtgataat atccgaaagc ttatttatca gatcttcagg cacgttgtag
     1921 taacacttat ttatctttac gttatacgca tctgccgtcg tttgtctgac aattatgcta
     1981 tattctttac cgtctgatac tttccactga gcagtaccct ttgaaagcat tccatttttt
     2041 gctgtatttt catcgaccgc atagcttaaa ctttcgttga ggagctggcg aaattctgtt
     2101 atagccgttt catctgttat atcccagttt ttcccctttg catatgggtc gccatactcg
     2161 gcagacacgc caaagccatt tatattgctg aaatcaagtt ctgtgatact atcattgtcc
     2221 accgaagctt cactttgttc agtgctttca ccgagcacaa cagagctgtt tgcaggcgtt
     2281 ggggcggcac tgctttctgt ggtatttccg caggctgtca ttacagctat tcctgacaaa
     2341 gtgaaaataa ataatatttt atatttcgtt ttgttcataa tatcgcctcc ataacagttt
     2401 atttacattt tgtaagatat actatgccat cacaatagtt gctgttaatc actacattat
     2461 cattacccca gccatcattt actatatacc atttatagat aatcttatta ccattatatc
     2521 gtagttttgt cccatatgct acaacccaat ggcctttata aatagggtga tttgccagac
     2581 ccgcaattac tggagtattt aacgcattca cattgtaata tgtcattccc caataatcac
     2641 ccgaacgtcg tgtaactgtt tcaagcgatg ttaatgacgt aacttgttta tacgcttttt
     2701 ctatgccaga agttaattct tcataattag ttccaatttt ttggggttca attatttttc
     2761 ttaaagtttc aaaggctttt tggggatcgt tagcatatgc agatttcaaa taatcatgag
     2821 acacataatc ataataatag aataacagaa tagtcgtagc taaagaacca caagcgttta
     2881 aatcgcctcc attgtaattt aaaagtctcg tttttccctt tactgtccat gaaacatctc
     2941 taatatttga ggttgaactg aatgtctgaa tagaatattc ggacgattct attggctcaa
     3001 aatcaaaatc actgacatta ttaccagtag tgatatcaaa gaactcacca ttttcttctg
     3061 tcacatattc taacggaccg ccataataga caatatcgtc ataatttgca tatggtgact
     3121 ctgctgttgg actatactct ataatgcaat catttacatc atagatgata tatccattct
     3181 cacagtctaa acaagttgcc acttgttcat tttcctgatt aagtagctgg tgtctgttca
     3241 caatatcgcc catgttatca ttctcaatgg tgtattgaat catactaggc acagaatagt
     3301 agctttcatt tgagtaagct aacacatcag ttgacattgt tcccatgata gcaaaagcta
     3361 atactgttga tataattttt ttcataatat cgcctcctgt aataattata ctcgaattgg
     3421 ataattattt caattggcaa atttaacata ttatttgttt atgttttgta caatttacta
     3481 aataaatcta aatattatta aaaatcgtca aaaaaatcgg cagcaggcac atgatagcgt
     3541 ctgctgccaa tagttttata ttgttttaag cattaaagca attatccaag aacaacaacg
     3601 atgtttgtct gctctgtcat cttgcactcg cacacgaagt tgccttcaac tgcttcgccg
     3661 ttgactgtca tagactttac gccgctctga acgtggtctg ggttctggat atcgattgaa
     3721 aggtgcttgc cacggaagtt cttttcgatc ttgaagccgt cccatgtgtg tgggatagat
     3781 gggcttatta caagtccctc tgcgtttggt ctcataccgc agataccctc tacgcagcct
     3841 accattactg ttgagcctgt acctgtcagc caatgaacgt gtgaacgtcc tgcatatggt
     3901 gaacgtgttg actctgtaaa ctgaccgtga acgtatggct caagtattct tacttctgcc
     3961 ttatcgttca tgttagcagg tgaagactcg aggaaatact cgaatgctct gtcgccgtga
     4021 ccgagaagtg actctgcaag gatagcccag ccctgtgtct gtgagaagat accaccgttc
     4081 tcctttgtat cagggttaaa gatgtgcatg agagcaccgt caaagtaatg atctacataa
     4141 ggtggctcca tgatctttac gccaaatgga gtattgagta tctcgtgtac cttttccata
     4201 gcagtttcag cctgcttatc agatgcgaag cctgagataa ctgaccaaga ctgtgggttg
     4261 agccacattg aagcctctgg atccttctta gcgccaacga tctcgccgtt ctctctgtag
     4321 cctctgataa atctatcctc gtcccagcaa gcttcaaggc tcttgccgag ttcaggctgt
     4381 accttgtcga tataagcagc gtactcatta tcgcctctgt caagagcata gcccttgatg
     4441 atgctcattg catagtagag ctggaatgca acgaatgttg actcaccctt cttaccaagt
     4501 ctgaggcagt cgttccagtc agcataaaga cctgctggca ttgtatgtgc gccaagtctt
     4561 tccattgaga agttgatagc tctcttaaga tgctcgtaaa ctgtatcctc gccgccgttt
     4621 gcgtatacga taacttcatc gaggaacgcc ttgtttccgc tctcgccgat atacttatta
     4681 actgttggga agagccagag agcgtcgtct gctctatagc atgggtgacc tgtttcctta
     4741 gcgtagatag agttctcatc gttctcgtcc gggcaagttt catggcctgc cttgtggtcg
     4801 aacttaacaa gtggaagtcc gccgccgtta tctacctgtg cagagatcat aaatctgatc
     4861 ttttcagcag caagctcagg attgatatgg ataataccct ggatatcctg aactgtatct
     4921 ctatagccat agccgtttct cagaccgcaa tagatgaatg atgcagctct tgaccagata
     4981 aatgtgatga agcactgata agcgttccat acgtttacca tgttattgaa ctcgtcagaa
     5041 ggagtttcga tctggaagtt attgagctga ccatgccaat aagcgataag ctcctttacc
     5101 tcagcgtcta ccttgccagg ctcgttatat gcagcgagta tctcgtctgc tacctttggg
     5161 ttcttctgac caacgacata gattatctcc tttgtttcac caggagcaag gataatgtca
     5221 gactggagag cgccgcaagc gtttgagtta tagttgagct tgttatcgca gaaccctctt
     5281 tctactgcaa cagggttgcc gtatgttctg taagagccga taaagctatc cagattaccg
     5341 ttataagcgg aaacaggagc gccaactacg ccgaaaaatc tttctctcca gttagagccg
     5401 ttctcgtcct taccgctgtt ctcattgata tgctgaacga tcttattgcc ttcaaagctt
     5461 gttcttgtga tgaacagtgt atactggagg tttacctgat cctgctcgta gttgttctca
     5521 tttgtgaact ctacaaagcc gaatgttgaa aggtttctgt atctgtcaga gttatttgtg
     5581 atcttagttc tccatacctc atatgttttg ttcagaggaa cataatatgt aagctcagag
     5641 tggatatctg catagtcaga cttgatagtt gtataagctg taccatggtg gcactctgtc
     5701 ttatactgat caagaggctt gcctacaggc tgccatgtgc atgaccaata atccttggcg
     5761 tcgttatctc tgatgtagat atatctgcca ggaagaccga tatttgaatt gaaacggtaa
     5821 cggattattc ttccgttagc accgctcttt acaaagctgt atccgccgcc gttgttggat
     5881 acgatagcac cgtactctgg tgagccgaga tagttagccc aaggtgcagg tgtgtcaggt
     5941 cttgtgatga cgtattcctt gtgttcaagg tcaaaataac catactgcat attttttata
     6001 cgctcctttt caatatttta aagttgtaaa cgtttcacca aaatggcgta aacgtttccc
     6061 tcatagatcc cgtcattatt cataacgctt atattatttt agcatacttc cgatagcttt
     6121 acaagggggt aaagccaaaa aaatgggcgt attttcaaaa aactttttcg ggattgttga
     6181 cttttgaaac ttaaacgatt gaaaatcgtc atgctttttg tcgttgttca agtcgcacta
     6241 ataactgata tttatttgta tatattggat attgacagtg atacttaaat atggtatatt
     6301 gtgtatataa taaattatag gaggtttacc attatgaaca ttaaaggaag agttttagct
     6361 atggtttctg cttgtgttgt gattttctca caaagttcaa tcgcaacgtt tgctcttgat
     6421 aaccaggagg ttcttacatt accaacttct gtttcagatt taaccagcgc tgaaaacatg
     6481 ggacaagtgg ttaaaattca caacttacat ggagcagatg aaggaaccat tgctttctgc
     6541 gctgaaatgg agtcgggata tgtgatatat gatttgtcag gaaatacaat agagtatagt
     6601 cctacatcat catctccata tagcgactta caaggcgatt tatattatgc tggtccactt
     6661 gaatacttga ctaaaacaag cactgattac aaaaatttgc gaacaggtga gattttaaca
     6721 gacgagcaat tcaacgaagt tactgaaagt tttactaatg agagcataaa attttcatca
     6781 actaatttta tgagttcaag tgcttctagg gctaattcag gatttcgcac agcagtttca
     6841 aaagtatctg gaacacctag aaaattaaac tacaacacat ctaatcaatg tggggcgtta
     6901 gcagctgtta tcaacttatg ctatattgat gactacaaag acaacaattg tttgagcgat
     6961 tcttattcta ataatcccaa aagtcttttc aacaccctta acaattatat tccgagagaa
     7021 acagaccgga atggaataat aaatggactt agcaacgcaa aaaaagataa aatatgtagt
     7081 tttacttcat cgcctgacgc ttactatggc ggtgattcat ggggattttg tttttacagg
     7141 atactcacgt caaattctcc aaccatatta cttattataa agcatccgaa ttatggtgga
     7201 gcaaagggga gaaatcattg ggtactaaca tatggcattg ttcaatgctt cgataataat
     7261 aataaattag ttgataaata ttttattgtt aacgatgggt atggaaaaaa tgatatacga
     7321 atccattaca catatcagga cgactgtgtt tacatttaag ggtaggtgac aaaacatgaa
     7381 aaagacagtt tatttggttt tagcgctctc actgcttctt tgctcatgct cacagacaac
     7441 agacaatgat aacaaaagca aggtcaatga cagcagtaca gttgtgacaa cagcttctac
     7501 aacaatgaca actaccacaa cttcatcatc aaaggttgat gaaaatgctg aaatttcggc
     7561 atattatatt catcactggt atggagaaga accatatgag ataacagaca aggacactat
     7621 ccacaagata actgcgtgga tagagaatgc taaatcggta tgcaaggatt atccttacat
     7681 tgacggctta gaatacggcg gtgctgacgc ttatgggatc tctctcgaaa aggatgaatc
     7741 aagtgatgcg tatgagagca tttgtgttat cgatgtttca cagtattcgt gggacacctc
     7801 aggcggtatg cactctgaaa gtaaaatatg caattttagc ataaaagatg atacaggcga
     7861 atttcaggat tacaatttgc caaaggaaat gatagatgag ataatgcccc ttataacaag
     7921 acctaatgtt gaatagcata caacacatac aaaaccggcg tgtatcttcg tgatacacgc
     7981 cggtcgtttc tattatgttt ttatgatacc ttgagccatg cgcaggcaaa atcaagtgct
     8041 tcataaagac ttgatttctc tgctacctta actatctgct tattcggagg cagtgtgctg
     8101 tctgttgcaa cttttcttat tcttacctta tttgcaacgt cgataccgcc gaccttttct
     8161 gtagaaagta tttctatcat gactacgaac tcgtcataca tatttccaaa atagatatta
     8221 ttcccttttc tcaccagagg aacgccctta taggtgaaaa aagctttctt ttcttctgcc
     8281 atttatctct ccgcctttct cataatatat ttctcttttt gtttactatt ttattatacc
     8341 atacttttca cataattaaa agagcttttg acaacttttt tgcgcaggtt ttcagccttg
     8401 ctttatgtgg gatacatatt gtatcacata ttcttcgcga aagctgtcga aacatgaccg
     8461 taggggcgaa cgctgttcgc ccgtcagatc atatgcttag catgggatat gcgttttaat
     8521 agttatcgtc cacagcctcg cctttaagaa gtttgtccat atttgcaacg aatgtatcta
     8581 cccaagttga ctgtattctg aatgaggttt caccatttag cttgactaca gaacgtcttg
     8641 aagaatcctt ataaatctcc ataacgtcct ttttgccgtc cttgcggtcg aatgttacag
     8701 tcagatagct ttcgccctca gggtctgtga agtagatctc atttgtcggg caacccataa
     8761 gatactgata gaaggtctta aagttgtcaa cgtcaagctc cttgccgtct gttgtgacct
     8821 ttgttacctg tgcggtagtt ttgccgtcag aatctgtatc agcacttgtg cctgagcttt
     8881 ccacctcata aacggtcttt ttaccattat agactacctc aacgttctta aggtcaacaa
     8941 ggtagtttga agtcataagg cttgtgataa cgtcctctgc tttgatcgtt gcccatggga
     9001 ggttatcggc tgaaacgagg tatatagcgt caacgccctt tacgccctca aagtagcaat
     9061 aatatgcaga cactgtatct gttgtttcac tgctgtcgcc tgcggtagca tagacagggt
     9121 cgcctatttt aagggtatag tcatagccgt ctcccgagag tcttactgtt gcgaacgggt
     9181 cggaaagtcc gtgctccttc attgcagttt catcaggtgc gatgacctca cagctctgtg
     9241 ctgtaagtcc ccacatacca tgggtagttg ctgaagaatt tgtgatgttc aggtatgaga
     9301 agataggctc tgtcattacc tgacctgaaa gtccctctat ctcgttcggg ttattttcaa
     9361 acgccatttc atagtccaga tcttttcttg taactgtttc cttgccatag tcaggccaat
     9421 cgtcgtcagc cggctttgtg agcaaagtaa ggttcgcata tgcggtcact ggatctgtat
     9481 agtatgaaag ctttgttccc attaccatat agacggtctt ttcatcgtca aggctcacat
     9541 agctgtatct ttcgttgctt ggtgaaacag agcctacatg aagggtagtg gcagtgccgt
     9601 cgctgtattc aactgtgaga tctgctgtgg gcttatcaag accgtatttt gacatatcct
     9661 caacgttttc ttcaacgagc tttttcgcct tgaagccgtc aagattcgat atcatggaag
     9721 cttctgcggt ggcatttgca ttgagagtgc caagctcagg tatgctccat gtggatttac
     9781 ctgactcgga cttattaacg gaatactcgc catgttcgtt tgaaaccttt acgcttgtta
     9841 tatcgtcgct tgactttgta aggataacaa cgctctcgtc ctgacttgaa actgccgctg
     9901 ttgacgatga gctggagtct gcgtctgatt tgcctgaggt ggagttgaga aacgccatta
     9961 ctcccaccag acaagcggct gtgactccgc agataatgat accctgcaat ttaccattca
    10021 tttgtgatta cctctgtgtt ttacttattc tttcttcttg cccagataac gatacccact
    10081 ataagaacta caacaggtac gccaaggcag aatgtccatt taagaactgt tttctgctgc
    10141 tgagtgatat cgaaaacatt gcctgtgata gtttttggtg tgatagtgat acctgcggtc
    10201 tttccagtaa ggccgtttat aacgcttatg aaatactcgc tgttctgata ctgtgaatac
    10261 tgcaagtagg tatcagacag cataccctct gaacctactg cgataacatt gctatagaga
    10321 gttttgttat cgtctgtaaa ttttgccttt gagccgacag ccatataatt ctgctgaccc
    10381 tttaccagag catcgcctat attcacctga cctgtggtct gtgatatatc aacatttgcg
    10441 gtataggcag aatctgagga cttgaggtag gcgtctgtgg acaccatatc ctgctcgtca
    10501 aagagtgtgt ttacagggcg gcagagtgct gaaaggatag ccggtttctc tgtagaaacg
    10561 tcctgagtga agttatcgga cacatctgac gcaactgtta cgcaaggtga gttataatac
    10621 ttgcctgaat cgctttcgca gataacgccc ttgccgacag aaagtccata ctctgacagg
    10681 aactcatcaa gattcggggt gtcctcctga ccataagaag cgatatagag gagctgtttg
    10741 ccaagatttc cgccattatt gaggaaatcg ctgacctttt tgatatcctc ctccagatag
    10801 tcagtcttag gagcagggac aaccacaacg tctgtatcgg cagggatatc ctgggaggtt
    10861 atatccactg tgttcacgtt atagccgtta gctgtaagaa gcgtttggaa atatgtgagc
    10921 tgtgtaagct cgcttctgcc tgtgaggata gaaacataga cagggttagg gtctgttact
    10981 gtcataagtg cagaagtgaa agcctgctct gcgtttgagg actcaacata gctgccgtaa
    11041 taggacagga atgaaaggtc gccgcctgcc tgctgggcaa gggtatctat gctgtagcct
    11101 gactgtgaaa gcttctgctc aaactcgtct gtgaatgtaa gaaggtcgag cataccgagc
    11161 tttcttgttc ggcttatctc tttgccgtca acctttgttt ttgtttcaaa tatcatatcg
    11221 aactggctga tagtatcggt atactcctta acgatctcag ggtgtgagtc gatgtcaacg
    11281 aaacgatatg tgatatggtg attgagctta caatagtttt tcaaaagctc aacagcctgc
    11341 ttattataag agctgtaatt tgtataagta ttctcgtctg cgaagatagt tacctgaacg
    11401 tctacgttca ctttctttac ataatcctct gtatcgtttg aaactgaata tatcttattt
    11461 gtagtcaggt cgatagtgat aggatatctg tcaaaaagca ttgttgtgac aacattcacc
    11521 aggacaagca gagcgacaaa aacgcaggtg aacactgttg ccattgttcc atgcttgagc
    11581 tttttggcat tgaacttttt gccgtccttt gtctttttct cttttttatc tttctttttg
    11641 tctttatctt ttttatcctt ttctgtatcc tttggagcgg tctttgctgc cttttcgtct
    11701 gctacaacgt ctttaagcag gtctgctcct gatacagaga tcttttcgtt attattctct
    11761 tccatttaaa cagccctcct ttagccccag cggcgttttt caagcactct caccgtgagg
    11821 aaaagaaatg ccactattgc actcagaaag aacacaacgt tagaaaaatc aaaaagtcca
    11881 taggtgaatg aataatatct ctcgccgaat gacacctttt cgcacactgt tgtgagccat
    11941 tcaaaaggca gaagattgcc tatagttgac aggaacgaca cgaagataag tgcgataaag
    12001 cttattactg cggcaactat ctggttttct gtgaaagatg agcagaagat accgatagcg
    12061 atataagcgc cgccaacaag tgccagagcg acgatattgc ccacaacgac gttccagtca
    12121 ggcgtaccga aagcggatac gacaactgca aaaacgcctg tgatagcgat agctatgata
    12181 tagataagga aagcggcaag gaatttacca acgactatgc tgccaagacc cacaggggcg
    12241 gtgagcagac actggtctgt ttttgttcgc ttttcctcag atatggtcct catggtgagg
    12301 acaggtataa gcactattat gattataaga agcgcactga aaacggaatc aagtcttgta
    12361 tagccgcttt caaggctgta gtttgcaaac attacgcctg aataggcata gaatgccgca
    12421 aggaaaatat aaccgatagg tgaggtaaaa tagcttctta cctctcgtct gaatattgcg
    12481 cccattagtc atttccctcc ttatcggaag tattttcaag ggcaggcaaa gcctcgccca
    12541 ttgtgagctt gaggaatata tcttcaagtg aaagctcaga ggtcttaagt tcaagcatat
    12601 accagccgtt tttaacaact gtttcaaaga cgtcacgtct gatatccacg ccttcttttg
    12661 cctctattct tatatcgttc acgccgtctt tatggcttgc gtcgcagatg actttttcaa
    12721 cgccctttat accctgcatt gctctgacga tcttatcctt atcgccctgt atctttgcag
    12781 tgagcttatg ctcgcctgag agcttctgtg agaggtgttc ctctgtatcg tttgcgacaa
    12841 ccttgccctc gttgataact attatcttat cgcagacagc ctgcacctca gagaggatat
    12901 gggaagaaag gataacggta tgatttttgc cgagctttct tataagagtt cttatctcga
    12961 ttatctgctt agggtcaaga cctactgtag gctcgtccag gataagaacc tttggattgc
    13021 cgataagagc ctgagcaaga cctactctct gtctataacc ttttgacagg ttcttgatta
    13081 ttctgttata aacatggtct atctttacaa gtccgcatat atctttaaga tgtgacacct
    13141 ttggaagctt acacttttta aggtcataga caaaatagag atattctttt acggtcatat
    13201 ccatataaag gggcggaagc tcagggagat agcctatctg ctttttagcc ttgatagggt
    13261 cttcaaggat atctacgccg tctataaggg ctttgccgtc tgtacaggat atgtagcctg
    13321 taaggatatt catggtggtg gattttcccg ctccgttagg accgaggaag ccgagaactt
    13381 cgccgtcctc cgctttgaaa gagatattat cgacagcttt tttgtcgccg tatctcttgg
    13441 taaggtttat aacctctatc attttatttc ctcctcttag gaaaagcatt cataagtctt
    13501 tacaatttta taatacttat gtgacagggg agtgatagct gtacgaaata cagagcattt
    13561 taagtaaatt taaagtagca aatactcaaa atgctccgct gttgaattat ttatctgtct
    13621 aaaagcgatg tatctatgta atttgtaggg gagcggtgca atttctgctt tgcagagatc
    13681 cgacatccca cttgtttgga aattatttcg cccctacaca tgttcaacat tttcgtagtt
    13741 tttattatga cagcttctca atgccctttc ttattcttgc aagagtatcc tccttgccga
    13801 tgagatatga tatctcaaca ccgccgcccg gagtgaacgc cttacctgaa actgcaactc
    13861 taagaggcca cagaacgatg ccgttcttta cgccaagctt gccgataagg tcaaagagtg
    13921 cgtcatggat attttcctct gtccagtcct caacgccctc gagaacaggg agaacttctt
    13981 tgagagattc cagagaattt tcagggttcg ttttcatctt cttatgtgta taaagagcta
    14041 tatcatactc agggagagcg tcgataaagt ctaccatttc aggtatctct gtgaaaagct
    14101 ctgttcttgc ctgcaagagc tttgctatct cgttaaggtc aacgtcctct ctatgaacgg
    14161 actgcttgat ataaggtata gcatactcag caaacttctc aggggtcata gcctttatat
    14221 acttgccgtt gatagcttta agcttagcag ggtcaaagat agcaggagac ttagaaagtc
    14281 cgtggatatc gaattcctta acaagctctt caagtgagaa tatctcgttc tcgccctttg
    14341 gcgcccagcc aagcagagcg atatagttta taacggcttc tttaagatag cccttcttca
    14401 taagatcctc ataggaagcg tcgccgtctc tctttgagag cttatgctgt ttatctttca
    14461 tgatatgctc aacatggata taggttggta tctcccagcc gaaagcctta tagagcaggt
    14521 tatacttagg tgcagaagca aggtactcgt tacctctaac aacgtgggtg atacccataa
    14581 ggtggtcgtc aacgacgttt gcaaagttat aggtaggcat accgtnnnnn nnnnnctggt
    14641 cgtcgagagt agagcagtca acagtgatat cgccgtaaag ctcgtcatgg aaagtaactt
    14701 cgccctctgt aggtattttc tgtctgataa cataaggaac acctgcgtca agcttttcct
    14761 ttatctcttc aggtgagagg ttacggcaat gtctgtcata cattggagcg ataccgcttg
    14821 cttcctgaat ggctcttact tcctcaagtc tttccttgtc gcagaagcaa taataagctt
    14881 cgcccttttc aacaagctcc tcggcatatt tcttgaacat acccattctc tgtgactgga
    14941 tataagggcc tacaggtccg ccgatatcag gaccttcgtc ccaattaaga ccaacgtcct
    15001 taagtgtctt atagatgacc tcaacagcac cgtcaacata acgctcctgg tctgtatcct
    15061 cgatacggag aataaagtct cctccattct gctttgcaat aagataggta aagagtgctg
    15121 ttctcaggtt acctatgtgc atataacctg ttggtgaagg tgcaaatctt gttcttacct
    15181 tgttcattta tatcttcctt tcaaaattag agtcttattt catatcctgc tcatcaaatg
    15241 caatttttac atatttcgca ggattatttg caatatactg ccttatttct gcgtgttgct
    15301 ttctgttttt gattatcgta tcataaaaag atttttgcca tatggaataa cctagttctt
    15361 ttgtgacagc ctctttgaaa tgttttatga cccttgaaag tgtcggtgtt tcacttgtag
    15421 gggcgaacag tgttcgcccg tttttcggct gtttttcaat tttgattata atatgcacat
    15481 ggtcaggcat gatacaagac atctctacaa aaacgtcggg atagtgttca gctatctctg
    15541 tgagttttct ctttacaagt tttcctgtaa cagaaagtgt tgggtgcaga atttcatttg
    15601 aagtcagcgg gcgaacactg ttcgccccta nnnnnnnnnn ctcccacagg acttggtatt
    15661 tttcagtaac acatatcgtc acaaaataca agccgttcgc agaataatca taaccctcaa
    15721 gacgtatttt ctttcgtctt ggcaaatctt cacttgtcat attttatttt cctcaaaata
    15781 tctgaccgcc tgctctttgt tttttgctat ctcctcacgg aggctgtcga agctgtcaaa
    15841 cattttttcg gggcggagaa attcatgcag ttcaagtttt agctttctgc cgtaaagatc
    15901 cccatgataa tccatgatga aggtttctgc aaggggagct gtttcaacat ggacggtagg
    15961 ctttacgcct atatttgtca cgcctgcata cttattttcg tctatttcaa cagcggagca
    16021 ataaacgccg aatttcggca ttaccctgcc ctttggtatc tgctgattta tcgtggggaa
    16081 atcccacgtt ctgccacgct gaaagccgtg ttccacttca aggcagaatc caaaatgata
    16141 acccagcatt tcatttgctt tggctatctc gccctcacgg acgcatcgtc ttatctcagt
    16201 ggaacttatg acctcgccgc ccatgagcag tggacttact atttccacat ctatatcata
    16261 tttttcaccg agccttttaa ggtcaacagc cttgccctgt gcgcctttgc cgaatgtaaa
    16321 atcgtttccg cacactgcac cgctgcaatt gagcctttct ttcagcacct ttttcacaaa
    16381 atcctccggt gacattcctt tgaactctgc aaaatcaggt gagaaaagca cttcgacccc
    16441 aagctctgcc atttttttat gcttttcctc gtcgctcata agcatttcta tcctgccgtc
    16501 atgacctttt gaggtcactg tgtcggtctt gaaacagaaa actgccgaat gtccgtcaag
    16561 gctctgagcc tttttcacag ccgttgttat tatgagctga tgtcctctgt ggactccgtc
    16621 aaaaagtcca agagcgcaaa ctgtcctttt tttggggata aacccgctgt ttgttatatc
    16681 cgtcaaaata tccctccgaa taaaacaaat tatcagaaat ttttgaagct tctgaagctg
    16741 ccctcatgaa aagcgccgat acccaggaag ctgttatcac tgccgtagac acggtagagc
    16801 ttgtcctctg acagatcttt tatccctgtc tgctcaggtc tgagctttac tccgtttcta
    16861 taaagctttg tttcccactg tccaagggta gttttttcgt agctttcaaa ggctctgtca
    16921 agtgggataa gcatttgtgg cagcttatcc tgctcacaaa tttcggctat ctcctctatg
    16981 gtatagcaat cctttatatc gacgccacct gaataggaac gctcaagaga agtcatcatt
    17041 ccgccgcagc caagcttttc acctatgtcg ctgataacgg agcgtatata agtacctttt
    17101 tcgcaatcaa ctttcattac gcccctgcgt gaagcttcgt catattctat tatatcaatg
    17161 ctgtcgatat ggacagctct cggctcacgc tctatttctt tgcctgctct tgcaagctca
    17221 tagagctttt tgccgttcac ttttattgca gaatacatag gcggcacctg catgatatcg
    17281 cctacaaaac tgctttttgc acattcaagg tcggctctgg taactgcctt gtcgcttgtt
    17341 tttaacgtct ttcctgtgct gtctaaggta tcggtgctta ccccaagctg aaagcctgcg
    17401 gtatagacct ttcgtctgtc ggggataata tcgcaggctt ttgtggctct gcccacgaaa
    17461 acaggcagaa cgcctgttgc catagggtca agggtccctg aatgacccaa tctttttatc
    17521 cttagaacgc ctctgagctt tgcgataacg tcaaaagaag tccagccctc gggctttttt
    17581 acaggcaaaa tgccgcagat attatccttg ctgttatcca agctcatcag cccaagacct
    17641 tttccgcttg cttgagaagc ttagccttta cttcttcaag gcttcctgtt atctcacagc
    17701 ctgcggctct tatgtgtcct ccgccgccaa actcacggca gaatgcagaa gcgtctatct
    17761 catcgtcgct tcttactgat attttaaaga cgttctcgtg tctttgctta actgttatgc
    17821 ctattttcac gccctcaacg gacaatggta tagaagcaag tccctcgaac tcggcagcgt
    17881 caacgccgct tttctccatt agctcttttg taagcactat catagagcat ttgccaccgc
    17941 agtaatactc catattcgct gtaacggact gctccaccat aagtctgccc ttgcttttta
    18001 catcaaacat ttttctgttg atataagcaa aatctatgcc atatgacata agctctgcac
    18061 aggcgatatg agcctgcgga gttgtgtttt catacttaaa gcagcctgta tccgttgcaa
    18121 tgcctgtata aaggcatctc gctatctgac gtgatatctt tctgcctgaa gctttgaata
    18181 tcttatacat tataagtgct gcggcagaag cgtctgcttc tacatatgac tgctttgcat
    18241 aatacttgtt tgagatgtgg tggtcgatgc aaagatcgat cgcaccctcc tcggcatact
    18301 ttgaaaggtt gctgccaaga a
//
LOCUS       DAWEMR010000030        18249 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1268_length_18249_cov_10.344179,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000030 DAWEMR010000000
VERSION     DAWEMR010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18249)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18249)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18249
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1268_length_18249_cov_10.344179"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1181..1190
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11370..11379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16561..16570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5546 a         3327 c         4154 g         5192 t
ORIGIN      
        1 tgggcttcca tatcgaggga atccacgggc tttgttgcgc tttttttaca gcgccataaa
       61 tttagcattt ttctttcttt ttgaactttg tcagattgat tatcaacgca aaaagtgcca
      121 ttaaaagaac tgccactatg agagtaactg ccgataattt aataaggctg tcagttaagc
      181 catacatgga tcttgtgagt gcattataca cagtgtcatt atgtatcgca agcccgtcat
      241 aatattttgt accctttagg atagcacacg gaacaagaat gagcattgag gaacagaaaa
      301 gtgaagtgcc tacccaataa agcaaaccgc ctatatgctt tctgtttact gctgccataa
      361 cgcctataag tataacagcc gttgcaataa gagctatcct tatttttctc gcaagcacaa
      421 gcaaatcttt aagctttgtg gaaatgccat taggtttatt tatatggctc aaaagcataa
      481 cgtcaaggtc ggattcaatt gcctgttcca cattttttat tgtcttctgc tttatgtctt
      541 caagttcctg agtatattta acgccttctt tttcagccca ttttgtataa tcgtctgaga
      601 tattcttttc aagaagcgta aagtctgcct taaactcagg aagttcgctc tttttgccaa
      661 gaatataact gaatgtatct tcaacatagc tataaatagc gtttgataca tcagttttgt
      721 taatcgactt cttcagggta tcagcttcaa caccagtgta ggcatattgt tgttcacaat
      781 atgtctgtat ctcctcgaaa gccgtgtcct cgatttttat cttgtgcata ctgttcgtat
      841 aggttgtcgg ttcaaagaaa atattgtctg ccgcaaacag aagtgacagc gcaaggaata
      901 aaaacgccaa aaacagcgag attatcagat taggcaggta gttgtatagt tttttgtttt
      961 tcacagatct taccctccta attcttatat tctttgctta tgactttgca gataacatat
     1021 agcctctgat ctgaaagacc catatagcca ttgttccagc aagtgtagag tatcagatca
     1081 tctacaggtg gatctgcggt cacgtcataa tacaaaaggg aagtatctgt atctggaagt
     1141 accttagttt ctgtgacctc ataagtaaat ttgccataat nnnnnnnnnn actagaatat
     1201 taggtaaata tctgaaataa gtgtggttat gagccgcaag gacaactctg cctgtagcac
     1261 ctatatatgc agaatagtta gattgcacaa ccccttttgc taggagatca tcagagtctc
     1321 cccagtaaac aggctctttg tcaatgcctg ccttatcatt ctttacggtc agctcggcat
     1381 atttgtcgcc gtaatacgga tagattatct catgctgttc cgtcttttta tcagaatctg
     1441 gtactgtgac ttttgttaat cccttcatag gcagtgcgtc tgcatttctg tatttattat
     1501 cctctgatgg gtttttcaaa tgagcgttct cattgaacgc tattgctgca tatccttgca
     1561 gctctatgaa ttttttataa cttaccagcg tcatgataga caaacaaaga gccaaaacta
     1621 aaagaggcgt caagaccatt acagccactg acgtcctctt attttttttc agtttgtttg
     1681 cgtcagacga ggaagcttta acctctttcc tgaccatatc ttagcactct ttgttcttct
     1741 taacagctac tgtaatacca aatgcactaa gagcaagtgt aagaaccgca gcaacctgaa
     1801 cagctgtcat gctgtttgca ccagtattaa gtgaaccgtt acctgtgttt gttcctatgt
     1861 ttgtattgcc atctgaatca acaacctgaa cgttccaatc cttgcttgta agctcgtcta
     1921 ccttgatagt aacaccaaag tgtttacctg tagctacgca agcatcagca atagcctttc
     1981 tgctatcctc gcccatttcc ttaaacacct gtctaagctg atcgtttgtt agtgaagcaa
     2041 gatctgttcc tgcaccaaaa agcttgtcag caacaggctg aacatatgta gcaccacaat
     2101 cagagagagc ctttaccatg taatcatagt cagcagagct gaactttgaa gcgtgagaat
     2161 caaggtagtt cttaagctct gatacgtggt tagaaggaac acctgctgat ttagctgcag
     2221 aaacaacatc ggcagaagaa gctgctgatg cgcttacaac cattgttgaa gcaatgatga
     2281 acgcagtaac agaagccgaa attgacttaa atatcttttt cataacacct atcacctttc
     2341 gtaatatttt cttataacaa tattatacac tattaaaatt cgattgtcaa tatttttatt
     2401 ggtatttttt tcagaaaaaa gcaaatattt ttacgataat ttgaaatagt tatgtgtaag
     2461 tgcgtatatg cgctatttca tgtaattgta tatcgctgga aaatgtaaaa gatcctcggc
     2521 attctcaaat aaaagacgta taacattact gcattggtgc ttttatgatt tttgtcagag
     2581 ctgatctcaa agcatttaga tataatttct acattaatta ttataattat acgccttgac
     2641 acgatatcat gccatggtgt ataattcttg tgtatgatat aattgatata atgtgcaagg
     2701 aggatatatg aaaaattatc tcaatgaaag cctttcagct caagaaagag ctgaagcact
     2761 tgtaaatgat atgaccgtag atgaggcggc ttcacagctt agatatgacg ctcctgctgt
     2821 tgagcggctc ggcattcctg cctataactg gtggaatgag ggtatccatg ggcttgcacg
     2881 aagcggtgtt gccaccatgt ttccacaggc aataggtctt gcggctatgt ttgacaccga
     2941 ccttgtgttc aaagtggcag atataacctc tacagaagct cgtgccaagt ataatgagta
     3001 ttcaaagcgt gatgacaggg atatatacaa aggtcttacc ctttgggcac ctaatataaa
     3061 tattttcaga gatccacgct ggggcagagg tcatgagact tacggtgaag acccttatct
     3121 taccgcagag tgcggcaagg catatgtaaa aggcttgcag ggcagtggca aggtactgaa
     3181 aactgccgct tgtgcaaagc attttgcagt acatagcgga cctgaggctg ttcgccatga
     3241 atttaatgca gaggttagtt ggaaagacct tgaagagacc tatctttatg cctttgagga
     3301 gcttgtgaaa aaagcaaagg tcgagggtgt aatgggtgcg tataaccgtg tgaatggcga
     3361 gccgtcatgt gctagtgagt ttctaatggg caagctcgac aaatggggtt ttgatggcta
     3421 ttttgtttct gattgctggg caataaggga tttccacgaa caccatatgg tgacgtcttc
     3481 tcctgtagag tcggccgcaa tggcacttaa agcaggctgt aatgtgaact gcggttgtac
     3541 atatgtacac ctgctttctg ctcttgacaa ggggcttgtg acagaagatg aaatccgtgc
     3601 ttcctgtgtt gctcttatga ggactcgcat aagacttggc atgtttgaca agtcaacgga
     3661 atatgacaac ataccataca gcgttgtttc ttgccctgag cataaagcag tatcctacca
     3721 gtgtgcagtg aagtccatgg tgttgctcaa aaacaagggt attcttccgc ttgacaaaaa
     3781 atcaatatca actatcgctg taataggtcc aaatgcggac agcagagcgg ctctcgaggg
     3841 caattataac ggcacagccg acaggtatgt tactttcctt gagggtatcg aggacgcatt
     3901 tgagggcagg gtgctttatg ctgaaggctg tcacttgtat aaggacagag tatcgggtct
     3961 tgcacaggca ggggatagat acgctgaagc tgaggcggcg gcggcaaatt ctgatgttgt
     4021 gatactttgc gttggtcttg acgccactat tgagggcgag gagggcgaca caggtaatga
     4081 gttttcatcg ggtgacaaga acgatctaag acttcctgaa agtcagcgcg agcttataaa
     4141 gcgtgtcgca aagatcggca agcctatgat agtggtatgt gcggcaggaa gctcaataaa
     4201 catcgagcat gattgtgacg ctcttatcca cgcatggtat ccgggttcag agggcggcaa
     4261 ggctattgcg gatattattt ttggtaaagt gtcgccatca ggaaaactgc ctgttacatt
     4321 ctataatagc gccgacaagc taccttgctt tactgattat gctatgtctg atagaacata
     4381 taggtacacg agagataaca ttctttatcc attcggctat ggtcttactt atggagatgt
     4441 tgttgttaca tcgcttagtt atgacgatgg caaagtcaat gtagaggtag aaaataacgg
     4501 tgccgatact tgtgatgtta tcgaacttta cattaaatca cattgtgata atgctccggt
     4561 ttatcctgtg ctgtgcggct tcaagaggat ttttgtcaaa aaaggtgaaa agcttgatgt
     4621 tgaaatcgaa gtacctgaca aagctttcac aacagtgtca gatatcggcg agagaaaaca
     4681 gtttgcaaaa gaattcaccc tctatgttgg tacgcatcag cctgatgaac ttagttgtca
     4741 gcttagtggt acaaactgta tgagtatcga gatagaacga taatgaaaag agatcttaca
     4801 aacggcagta tcataagctt gcttcttatt gttttggtga aagaaatgaa cgccgcctga
     4861 aaggctgtct gaacatatca tttcagatga taggctctgt tactttggtg acagagccta
     4921 tcacattgtg cttttccaat gagattttga aacttttgca tactccaatt aaaataatgc
     4981 tgttgacatc tgattatgtt agaattatcc tgtcatgaat tccgacatac ttatgataca
     5041 aagactggta aatagttttg gaacgactgt aatggcggct tttacagtag ccgttcataa
     5101 ttgttaaatt aagctgtcag cttatatgat tttccaacaa atacttggct gattttgttt
     5161 atcttattgt atgttataac aaaaaaccat ttttttgttg aaatacaaaa aaacgtatcg
     5221 tttaaagttg actttttgaa tattattgta cggtttattt tttgggaaaa taacgaaaat
     5281 atcaacaata gggtgaataa ttcaaatgaa aaaaaattac actatcaagg atcttctggt
     5341 acttcttctc ggaaaggttt ggtatatcgt tgccgcttca atagtgtgtg cgatactggc
     5401 ccttttcgta tcaaaatttg ttatgagtaa gcagtatcag tcacacgttt caatgtatgt
     5461 taaaagtgct gataatcagt caacgggtca ggataagact aatgttgact tacaggacat
     5521 taacgcttca aagtcattgg tacagaccta cattgttata cttactgatg acccggtaat
     5581 gaacgaggta agcgataaac ttcttgagat gtatactcct gaacagcttg ctccttattt
     5641 tactgtaaca agcggcaggg tttcaaatga cggcctgaga aatgcttata gcatgaccgc
     5701 tgtaaatcag acagaggtac ttcagataac tgccacaaca accaatgcaa agctttcttc
     5761 tgacctttgt aacatcatgg ctgacgtcgc tccgagcttt cttataagag ttatcggtgc
     5821 aggctcagtt gaaaagatag gtgatgctga ggtttacaat acacctgttt ctcctaacac
     5881 cactaaaaat gttgcccttg gctttgttgg tggcttgatg gtggctgttc tagtgatact
     5941 tgttatcgac ttcttcgaca acactgtaaa gaattctgat gagcttggtg aaatttatca
     6001 gaagcctatc gttggtgaga taatgaatat tggcggaaag acttctaaaa aggatgaagg
     6061 ttcttctgct gacagaaaga aacagcttct gttcaataat aaggatatac cgtttaatat
     6121 tgtagagaac tacaaaacaa tgcgtacaaa ccttatgttt acactttcta caacacgaaa
     6181 taagatagtt gtcgtttcta gccctaatcc aagtgagggt aagtctgtaa cagttgctaa
     6241 ccttgcagct tcaatggctg aaacagaaaa taaggttctt cttattgacg ctgatatgag
     6301 aagacctgtt cagcacagaa acttcaagtt aaaaaacaat gttggacttt caacaatact
     6361 gagtggtgaa agcacatttg ataagtgtgt gcatgaaacc aagaatggca attttgctgt
     6421 tcttacatca ggcgttttgc ctccaaaccc gtcagagctt cttgcttctg agaaaatgcg
     6481 tgaattgctt gaatatgttc agcataagta tgattatgtt attgtcgacg gaaccccgct
     6541 gcttcctgta actgacgtaa tgagccttgc agatgaggtc gcaggcgttc ttctcattgc
     6601 aagatatggc agaactacat atcaggaaat agaggagtct atgaagaagc ttgagatagg
     6661 cgattgcaat ctgcttggtt tcgttcttaa tgatatttcc gcaaagaacg gttctcatta
     6721 cggatacaag tatagctaca agtataaata tgactacagc tataaatacg gtggtaagga
     6781 tgcttctgcc gaaagttaaa tgacatggag atgttagatt gactaccgat tatcacagcc
     6841 acatactgcc gaatattgat gacggctctg acagcatcaa aacatcactt aaaatgataa
     6901 aaatgatgaa acagcagggc ataggtataa ttgtggcaac accgcatttt tatatgcaca
     6961 gggaagcttc tttagacagt tatcttgtca aacggcaaaa tgcttatgag aagctcatgg
     7021 catctgaccc tgccgtgaag gatatacata ttggtgctga ggtggctata gaaaagggca
     7081 tcagcgtatt aggagaactt gaaaagctgt gtatcagcgg aacaagactt attcttcttg
     7141 aaccaccctt cactcagtat aacagttgga tcgtcgagga gatagacgag atagcaaatt
     7201 ctcgaaggct tattccgata atcgcccatg tacatagata tatgggcgtt tattcaaagt
     7261 cagatctgaa aagtctgatg tctgtggatg cagtttttca gataaatgca gaagcttttg
     7321 agcatttttc cacaagacgt tttgttaata agcttatttc aagtggcaga gattttgtct
     7381 ttggaagcga tgctcatgat cttgatgata gaaaacctaa ttttgatctt atagctaaaa
     7441 gatctggaag tgaaaatata agacgttctg ataaagtact catgtctgct atggctgacg
     7501 aagaaagaat gtttgctttt taagtataga aaaaatattg atgatattat aaatagcctg
     7561 cactgtgcca ttgaggggtg gccatggtgt gatcttattc attgctttaa ttgagcggag
     7621 gtatgtttta tttatggaga aaacaagtga aaacacagtt caggtgtgga aaaacaaaaa
     7681 gataaaacat tggatgctta ttgcattctt tctgtgtttc tatgatattg ttgccgtaaa
     7741 tctatcctac attttcggac ttttcatcag atttgacctt actttttctg caataccaaa
     7801 ggagtatatg cttgcctatg taaagtttgc tccaatatat acagctttct gtgttgtggt
     7861 gttttttgtg ttcaagcttt ataatagttt gtggagattt gctagcattg gcgaactgtc
     7921 aaggatattt ttggcctcga tagttactac catattccag gctgtgggta taacattact
     7981 cttcatgaga atgcctgtgg cttactatat agtaggagcg ggaactcagt tcgtgcttat
     8041 aacagcagtg cgttttgcct atagatatgt taccctttta agagcaaaac aggtaacaaa
     8101 tcaggtggca gtgcataatg caatggtcat tggtgcaggt gcttcgggtc agatgatcct
     8161 taaagagctt acgatgtcag aacgtgcaaa tgcaagacca ctctgcataa tcgatgataa
     8221 cccaaataag tggggaagaa atatcggagg agttcctatc gtcggtggcc gtgattgcat
     8281 tatggaaagc gtcaaaaagt ataatattga tcagatcctt tttgcaattc caactgcatc
     8341 acctgagaac aaaagggata tccttaatat ttgtaaggaa acaggctgtg agatgaaaca
     8401 gcttccgggc gtttatcaga taacaaatgg tgaggtgctt ctcagcaaga tgaagcctgt
     8461 tgcggtcgag gatctgcttg gaagagagcc tatcagagta aacatggacg agatattcca
     8521 gcaccttaag ggtaagacca tattggtaac aggtggcggt ggctctatcg gaagcgagct
     8581 ttgccgacag atagcaggtc atgagcctaa acagcttatc atatttgaca tttatgaaaa
     8641 caatgcttat gaaatagagc aggagctaaa acgtaaatat ggcagtaaac tcaatcttgt
     8701 gacgcttatc ggctctgttc gtgacagcag acgtatcaat gacgtttttg aaaaatacaa
     8761 gcctgatatc gtttatcacg ctgcagcaca taagcacgtt ccgcttatgg aaacaagccc
     8821 taatgaagct atcaaaaaca atgttgttgg tacatacaag acagcctatg cagctttgaa
     8881 gcatggcaca aagaggtttg tgcttataag caccgataag gctgtaaacc ctactaatat
     8941 tatgggtgca agtaaaagac tctgcgaaat ggtcatccag agcatggacg ctatcagcaa
     9001 ggcaggcaga actgatcttc tacctatgct ccatgcacac gttgatgaaa tgacagacgg
     9061 tatgcttgag aacgatccga tagacgagat agctgttgat aatatagaga gcagtgaagc
     9121 ggttaagatc gaaagcgttg gcaataagga cagaaatggc acacagtttg ttgcagttcg
     9181 ttttggaaac gttcttggaa gcaacggctc tgtaatccca ctgttcaaaa agcagataga
     9241 agcaggcggt cctgttacag ttactcaccc tgatatcatg agatatttca tgactatacc
     9301 tgaggctgtg agccttgttc ttcaggcagg cacatacgca tggggcggcg agatatttgt
     9361 ccttgatatg ggtgcgcctg tgaagataga cacacttgcg agaaatctta tcagactttc
     9421 aggatataag cctgatgtag atatcaagat agtttactca ggtctgcgtc ctggcgagaa
     9481 gctttttgag gagaagctta tggcagagga gggcatgatg aaaacggata atgagcttat
     9541 ccatatcgga aagcctatac cgtttgacac agaaacgttc cttggtcagc ttggggagct
     9601 tgctcgtgca agctataaca atgacgaaaa tatcgttgaa atggtcgaaa agatcgttcc
     9661 aacattcaat cctgtaggtg ataaaccaac aggtaatgaa aaatatggca gaaacgatgt
     9721 tgcagtttct gcggcaaaat aataaaagat ccatatttca gcactgcggg gatttctgcg
     9781 gtgctgtttt tatccctaaa atttcacact ttttcttgat gctacttgaa aaaaggcagt
     9841 ttatgtggta taatgaataa tgaatgtaaa tttgcgtaaa ggactatttc tatatgaaaa
     9901 aatggagaat aaacagacct gaccctgtga aaactgcaga gtttatgaac aagtgtgact
     9961 tgaaaagcct taccctagac gtcatgactt caaggggttt cactgacttt gacagccttg
    10021 ctgatttttt taaaggcgaa gaacttagtg atccttttct tataaaagat atggctatag
    10081 ccgccgaggt gataaacaag gcggttgatc agtatgacct tatttgtatt tacggtgact
    10141 atgactgcga tggagttacg tccaccacta tactttacaa ctatcttgaa agcatgggcg
    10201 caaacattat gtactatatt cctgagcgtg aggctggata tggcatgaac atggaagcta
    10261 ttgaaatgct tgcagaaaag ggtgttaagc ttatcgtcac tgtggataac ggcatttccg
    10321 cggttgagga agctgagcgt attgctgagc ttgatatgga acttgttatc acagatcacc
    10381 accaaccgcc tgagaagctt ccgagggcaa gggctatcgt taatccgcat cgtgcagatt
    10441 gtccttcgtc atacaaagac cttgcaggag ttggtgtagc tttcaagctt tgtgctgcac
    10501 ttgacggcgg cagttacgac actgttatgg aacagtatgc cgacatttgt gctatcggta
    10561 cagttgcgga cgttgtgcca ctcactgggg aaaacagaac tattgtcaag cgtggtcttg
    10621 aatatcttgc aaacaccgaa atattgggac taaactatct tatagataaa gcaaagcttg
    10681 atagaaatgc tcttgactca acaggcattg cttttaggat tgcccctgtt ataaacgctt
    10741 cgggcagatt tggctcacca cttacagcgg taaaaacact tctaagcgaa gacccggagg
    10801 acgctgaaaa ttatgttgac actcttttaa atcttaacgc ccagcgcaag cagacggaaa
    10861 cagagataat gacagaaata gtaaactata taaacgctca ccctgaaact cttgaccata
    10921 gagtgcttgt gctttcaggc aagggctggc accacggagt tattggcatt gtttcttcac
    10981 gcattttgga attttacgga aagccgaatg ttataatctc cattgatgat gatggaaacg
    11041 cacgaggctc tgcgagaagc gtaaagggct tcaatatatt caagtgtttt cagcattgcg
    11101 gagagctgct tgataaattt ggtggacatg agtgtgcagg aggtctgtcg ctgaaggctg
    11161 agaacatcga aaaattcact caaatggtgt atgaatacag cgatcctatg gagaaattcc
    11221 ctagcgttga gcttatagcg gataagcttc ttatgccgca ggatatcaac cttgataatg
    11281 taaagggtct tgcagcaatg gagccttttg gtgcagagaa ccctgctccc gtttttgcaa
    11341 tgcttggagt aagagtagat aagataatan nnnnnnnnng ttgtcacagg gcaagcatac
    11401 aaagatcgag ttttcatacg gaagctatag aggacaggct cttattttct cccttgcacc
    11461 tgaaaaagct tgtttcgcag ttggcgataa gcttgatatg ctcgtggagc ttggaataaa
    11521 tgtgttcaat aaccgtgagt ccatatccat aagagttatc gaccatagat tgagtggagt
    11581 aaagcaggaa agatattttg cggcaaagga ttgttatgaa aagctcatgc gtggggaaca
    11641 gcttcctgca agttttataa gaaaaataat cccgacaagg caggagctta tcggcgtata
    11701 caaatacata agtgcagtta agaatattac gttggataat cttttcatga aaatatcagg
    11761 cgacagcatg aattattgca aactgcatat ttgcgttgat attttccgtg ataagggtct
    11821 tatcgagttc agacccgcaa ctatgaaaat aagctacgtt gtgccgaaac aaaaggtaaa
    11881 tcttgaggac tctgaaactc ttgttaagct gaatgaaatg cttggaaagg caggtaacta
    11941 aaatgtcaga taaagagaat attttgcttg atcctgctga aatggataag atagaaaata
    12001 tgcctgagat aaaaactgtt agtgaagaag atcttcctgc cgaagctgag gttaaagagc
    12061 agtttgaaga aaagcctgct cctacggctt ctcctgacta tatcgatgct gtgcctgaag
    12121 cttcaaagcc tgagcatata ggtgagaggt cggtgtgttc cattgatgcg cttattcaga
    12181 aaatacttga tggcgagagg cagtacgatc tgtcaaaaat cgtttccgcc tatgaacttg
    12241 ctgaaaagta tcatcacaat cagaaaagag agtctggcga gccgtatata acccacccgc
    12301 tgtctgttgc atatattctt ttggagcttg gcatggatac tgatactatt tgtgcagcgc
    12361 ttcttcatga cgttgtggag gacactccat gcactcttga agaattacag aagaattttg
    12421 gctcagacgt tgcaatgctt gtaaatggcg ttacaaagct taaaaaggta gaaactttca
    12481 ccaaagacga gcagaaagcc gagaatatca gaaaaatact ccttgcaatg agcgaggata
    12541 ttcgtgttat tatcataaag cttgcagata gacttcacaa tatgcgtact cttaattatt
    12601 gcaaagattc aaaacgcaga actatcgccc atgaaacaat gaacatctat gcaccgatag
    12661 ctcaccgcct tggaatacgc tcaattaaag atgagtttga agatcttgct ttttactatc
    12721 ttgatcctta tgcttatgcc gagatagacg agcaaatgca gctcagaaag ggcagccgtg
    12781 aacagcttgt tgaaaacatc aagcacaaga tacatgacag gcttgaaaag gattttgacc
    12841 ctgttcccct tatcgagggt cgagttaaaa gcaattatgg tatttataaa aaagtgtatc
    12901 gtgacggcaa ggagatagac cagatatatg atcgctatgc cgtaagaatt atcgtcaata
    12961 cagttaccga gtgctataac gtacttggca taatccacga tatgttcaga cctatcccaa
    13021 acagatttaa ggattatatt tccacgccta aggctaatat gtatcaatct cttcacacaa
    13081 ctgttatcgg cagagaggga atacctttcg aggttcagat aagaacatgg gaaatgcaca
    13141 gaacggctga atatggtatc gccgcccatt ggaaatataa agagggcgta aggggtagct
    13201 caaaggacga tcagcgcctt gcatggataa gacagataat cgagtcacag caggagtcaa
    13261 acgacgttga ggaaatagta agggctatca aaaatgacct tgcccctgag gacgtctttg
    13321 ctttcactcc aaagggcgat atgataacac tgcctgtcgg atcaactgtt atcgactttg
    13381 cttatgctat ccacacacag gtgggtcata agatgtgcgg cgcaaaggta gacaagaaaa
    13441 tggtgtctta tgattatcag ataaaaacag gcgagataat cgagatactt actaccaacg
    13501 ttgaaggtca tggtccaagc cgttcatggc ttaatatctg caagaccaat gaagccaaat
    13561 ccaagatacg ttcatggttc aaaaaggaaa gacgagagga aaatatcttt gagggcagaa
    13621 atgctcttga aagagagttc cgcagaaata atataagagt gcctgaggaa gaacttgagg
    13681 actttctcaa aatggatatg cacagacaca gttgtgatac tcttgatgat ttctttgcag
    13741 ccataggtta cggtggtgtt cagctttcaa aggtgatgca aaggctgaaa tctgagtata
    13801 ataagaaata tggtgaaaag gctcagcctg atacttctga ccttgaaaat aagataaaga
    13861 cttccaaaaa ttccactggt gttatcgttg acggcatcga caactgcgct attaagtttg
    13921 cacagtgctg taatccgctt ccaggtgacg aaatagtcgg ctttatcaca aggggtcacg
    13981 gcatatccgt gcataaaaag gattgcgtga actatctttc gcagaaagat gatcctgaaa
    14041 atgccgctcg ttggataaat gttaagtggg aaagttctga aaagcatacg ggctatttta
    14101 agtgtacttt ggatattgta gcagttgaca gaataggtct gctggcagac gtttcgtcag
    14161 cgcttgcaat gatcaatatt tttatctatg agtcaacctc aagggaactg aaaaacggca
    14221 atgcaatgct ttccgtaact gtcagcattg caggaatgga acagcttaac aatgttataa
    14281 acaagcttca gaaaataaag aacgttattt ctgttgaaag aagcggtaaa taagaaagga
    14341 cagataatga aaatatttag gctcaaacca ttgagtgttt gtgaaacgaa cagctatatt
    14401 gttgcaagcg aagaaaataa ctgcgttctt atcgatgcac ctgccgaccc tgattatatt
    14461 ctcggtgaga tagagagtca cggtcttacg cttaaaaaga tctttcttac ccacggtcat
    14521 tttgaccata tcggtgctgt ggctgacctt gtagacagga caggctgcga ggtgtatatc
    14581 cacattatgg ataagcctaa gcttacagat gacgcaggaa tgcttgctaa tcttttcaga
    14641 ataagaggac acagaaatta cacaggcaag gtcaatgtgt ttacagagga cgatatacta
    14701 aagcttgacg agcttgaatt tgacgcgttg gaaacgccgg gacatacaag cggttctgtt
    14761 tgtttcatat gcggcatgaa tatgttttcg ggagataccc tattttcaag aagtgttggc
    14821 agaactgata tgcctgatgg aagcagcaca gcccttatga agtcgcttat gaaaatagct
    14881 gaccttggtg gaaatctaac tgtttatcct ggtcacatga atgtcacgac acttgacgct
    14941 gagagaaagt ataacccata tctcaggcag gctgcggagg ctttgaaatg actctgatat
    15001 tcagcggcaa cgattataaa tatgagcttg agggagtaat gaagcttttt atccctgcaa
    15061 cgctctttac ccacgttttt tctgacagca tagatacaga ggacgattat gtgttcgctc
    15121 agaaaaagga taacgctgac aatgtttgtt tgagcgttaa ggtgagatat gacggcaaga
    15181 cttgcgaaaa agaggagttt gtgcattttg aaagcgacat ggaactttca ctttcaagac
    15241 ttctttttaa ggcaatgagc gagataacag gcattgtgcc gaaatggggc gttataacgg
    15301 gaataagacc tgtgaagcgt gtgaatgata tgctcagtga gggcatgaac aaggcggaga
    15361 tatttaaagc catggagagc aggtatcttt gcagcgaaga aaagtgcgat attgcataca
    15421 aaactgctat aacccagaag cctgtcctcg atgagcttga aaaggacagc tttagtcttt
    15481 atgtgtcagt gccgttttgt cccacaagat gctcctactg ttctttcgtt tcgcaatcta
    15541 tcgatggctg tatgaagctt atccctgagt atgtgaacaa gctgtgtgag gagatagttt
    15601 atactgcgaa gataacgaaa aagcttggtt taaagcttga caccgtatat tttggcggcg
    15661 gcactcctac cacgctcatt gctgctcagc ttgacagagt tatgaaggtc attgcaaaca
    15721 gctttgatat gtcaactgtc cgtgaataca ctgttgaagc gggaagacct gacaccataa
    15781 ctgaagaaaa gctgaaggtg cttaaagcaa acggctgtgg cagagtatct ataaatccac
    15841 agactttgaa tgacagcgtt cttgaggcta ttggcagaaa gcacacaact gcacagtttt
    15901 ttgacagctt tgaccttgca agaaaagttg gttttgactc tattaatact gatataatcg
    15961 caggtctgcc aacagacaca gtggagagct ttgagaatac cattaataag cttatcgagc
    16021 ttgctcctga gaatatcact gtacacacac tttccataaa gcgtgcggcc aggctaaatc
    16081 acagcggtga cagggaagtg ctgaaaaatc cagccgataa aatggtagag tatgccacaa
    16141 aaagacttct tgaaagcggc tatctgcctt attatctcta cagacagaag aatatgcttg
    16201 aaaatcttga aaatataggc tggacaaagc agggtcacga gagtttgtat aatatctata
    16261 taatggaaga ggttcaaacc attcttgcaa tgggtgcagg tagctcgaca aagcttgttg
    16321 acaaagaggg cggacgtttg gagcgtgtgt tcaattacaa atttccgctt gaatacaata
    16381 agcattttga gcttatgctt aagcgaaaaa atgagataga ggaattttat gctaaagaaa
    16441 aatgagatta tcaggcttga aataagcggt atgacaaatg agggcaacgg agtaggaaag
    16501 cacgagggca ttgctgtttt cgtgcctttt actgttattg gcgacgttat cgaatgcagg
    16561 nnnnnnnnnn atgcaggata gtcaaggtct gcaaaacgta ttgctatggc attatcgaaa
    16621 atattgtaag tggttcggtt gaaagaacga agaacgattg ccctgtttat tcaaggtgtg
    16681 gtggttgttg ttttcgtcac atgagctatg aggaagaatg ccgtgtaaaa gagcagttca
    16741 taaaggattc atttgagcgt attggtaagc tttatcctga atatgacagc tttgagggtt
    16801 gtaaacagct tgttggctat cgcaacaagg cgcagtaccc tgtagcagag caggacggca
    16861 gggcagtttg cggattctat tcaaggcgtt ctcattatgt atgcgatcac accgattgtg
    16921 cattacagcc tgccatattt aaggctgtcg ctgatgacat aatggcttat gtgaacgagt
    16981 gtaagataaa ggcatacaac gaggaaacgg gaagcggtct gctcaggcat atttatctca
    17041 ggcgtggcga acactcaggt gagataatgg tctgtcttgt tataactgac cttaaaaagc
    17101 gtggcgtatt tgatgcgctt gtgggtgagc tttgcaaaaa atatgctgat ataaagagta
    17161 tagttttcaa tgagaatagt cgaaagacca actgtatact cggacagaag cttgttactg
    17221 cgtacggaag cgacacgata cacgatacca tgtgtggaaa tgatatagag atatcaccgc
    17281 tgtcatttta tcaggtaaat acaattcagg cggagcggct ttatgaaatt gccgcagatt
    17341 atgctcagct gacaggaaat gaaactcttc ttgatcttta ctgcggtgca ggaacgattg
    17401 gtttgtcaat gagcaaaaag gtgaaaaagc tcataggtgt tgagataata caatctgcat
    17461 tagacaatgc aaaacgaaat gccgcagcga acaatgtcac aaatgcggaa tttatatgtg
    17521 gtgacgcagg aaagatagca gagatacttt acagccgtgg cgaacgacca gatgtgataa
    17581 tagctgaccc tgcaagaaag ggctgcacga gggacgcact ggagtacatg gccaaaatgt
    17641 cccctgacag gatagttatg atatcttgca atcacgctac tgctgctagg gactgtgctg
    17701 tgctggaaga gttggggtat aagacggtca aggtcaaggg cgtggatctg tttgggagga
    17761 cggggcacgt ggagtgcgta gttctgttga ctaaaacaca taagtaaaag tgtgaaaatg
    17821 gcttgaaata aggggatttc gatagctttg gttactggaa ataatacctc aaaaaatgtc
    17881 ttaaaagcct tgaaaaatct aggaaatttt atggttggat agacaggaat attgagattg
    17941 ggtcgattat acagaagtgt ttatttgtga tttgtgaaaa ataaacgcag taatgtggag
    18001 caaacggaag atggttcaaa aagaaagaaa accaagtaca gttctaaaaa gcataattaa
    18061 cacaattagg tgatgaaaat tagacaaatt tttgtattct atattacttg ataatataga
    18121 ggaaaagagt gatatatggt agtaccaaaa tagtaacgga ggtattgtct atatgcttta
    18181 cttaaaaaca tcagttaaaa acggaataat cagtctttat cctttgaacc tagatgaaat
    18241 gtattataa
//
LOCUS       DAWEMR010000031        18231 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1269_length_18231_cov_11.456536,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000031 DAWEMR010000000
VERSION     DAWEMR010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18231)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18231)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18231
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1269_length_18231_cov_11.456536"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6770..6779
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5047 a         3617 c         4485 g         5072 t
ORIGIN      
        1 ggtatgcaat actgaatatt gaggtaatat gtgctgcccg aaatcttatg tcaggagagt
       61 ataaaatgga aagacagttt tatgacgaat gggaaggctt taagcccggc agatggtcaa
      121 acacttctat caatcttcgt gattttatcc agaagaactt cacaccatat gacggtgacg
      181 acagcttcct tacaggccct acagaagcaa ctactaagct ctgggaacag gtaatggagc
      241 tttcaaagaa ggagagagaa gccggcggtg ttctcgatat ggacactaag atcgtttcta
      301 ctatcacttc acacggccct ggttacctta acaaggatct tgaaaagatc gttggtttcc
      361 agactgacaa gcctttcaag cgttctcttc agcctttcgg cggtatcaga atggctatga
      421 atgcttgcga gcagaacggc tacacagttg atcctgaagt tgtaaagatc tttactgaat
      481 atagaaaaac tcacaatcag ggcgtttttg acgcttacac acctgagatg aagcttgcca
      541 gacacgctgc tatcatcaca ggccttccag acgcttacgg cagaggaaga atcataggcg
      601 actacagaag agttgctctt tatggtatag actatctcgt tgaggacaag aaggatcagc
      661 ttgctacttc actggtaaga atgacttcta agaacatcag actcagagaa gagctttctg
      721 agcagatcag agcccttggc gaactcaaga agttgggcga gatctacggc tacgatatct
      781 cacgtcctgc taagaacgct aaggaagcta tccagtggct gtacttcggc taccttgctg
      841 ctgttaagga gcagaacggt gctgcgatgt cacttggcag aacttctaca tttatcgata
      901 tatacatcaa gagagatatg gacagaggta tcctcacaga gcaggaggct caggagcttg
      961 tcgaccactt catcatgaag ctcagacttg ttaagttcgc tagaacacct gagtacaact
     1021 cactgttctc aggcgaccca acttgggtaa cagagtctat cggtggcgtt tctatcgacg
     1081 gcgttccaat ggtaacaaag acttcattca gatatctcca cacacttgag aaccttggtc
     1141 ctgcaccaga gccaaacatg acagttctgt ggagcacaaa gctgccttca aacttcaaga
     1201 agttctgtgc taagacttct atcaagactt ctgctatcca gtacgagaac gacgacctta
     1261 tgagagttac acacggtgac gactacgcta tcgcttgctg tgtatcttca atgagaattg
     1321 gtaaggaaat gcagttcttc ggtgcaagag ctaaccttgc taagtgcttg ctttatgcga
     1381 tcaacggcgg tgttgacgaa agacttaaga tacaggttgg tcctaagtat cgtcctgtta
     1441 caggtgatta cctcgattat gatgatgtaa tggctaagta tgacgatatg atggagtggc
     1501 tggctggtct gtatgtaaat acactgaacg ttatccacta tatgcacgat aagtattctt
     1561 atgagagagt tcagatggct cttcatgaca gagatgtcaa gagatacttc gctacaggta
     1621 tcgcaggtct gtcagttgtt gctgactcac tttctgctat caagtacgct aaggtaaagt
     1681 gcatcagaga tgaggacgga atcgttacag attacgaagt tgagggcgat ttccctaagt
     1741 atggcaacaa cgacgagaga gttgacaaga tcgcagttga cctcgttaga acatttatgg
     1801 acaagatcag aaagcaccac acatacagag atggtgttcc tacaatgtct atccttacta
     1861 tcacatctaa cgttgtttat ggtaagaaga ctggttctac acctgacgga agaaagatcg
     1921 gtgtgcctct tgctcctggt gctaacccaa tgcacggcag agatactcac ggtgcatctg
     1981 catcactttc ttcagttgct aagctgccat tcagacacgc tcaggacggt atctcaaaca
     2041 cattctctat cattcctgac gctcttggca aggacgacaa ggtattcatg ggcgaccttg
     2101 atatcgaaag catagctaag gagcttaacg aagatggggt ttaatgttaa aaaggacgag
     2161 gacgaggctc aggtaaacga gcttgttgac gtcattgaca gacttatgat gggcggtcag
     2221 ggtcatctta cgatagacat tgacgaaact gaagaaggtc tgaacgttaa aacttacagc
     2281 acttccgatt gtggcggtga cggcaagacg gcttgctgtc agcctacaga agaggatgct
     2341 gttgacagag atgaagatta actccctatt ttagaaaacg gcggaggttc gttctccgct
     2401 tgttttccat aaatactaag aaaggaatgt aatactatgg caaactttga accaacagag
     2461 gcacaggtag acaaccttat cggacttctt gacggatatg ctgaaaaagg cggtcatcac
     2521 cttaatgtaa atgtacttaa cagagaaact cttctggacg ctcaggctca ccctgagaag
     2581 tatccacagc tcactatcag agtttcaggc tacgctgtaa actttgtaaa gcttactaag
     2641 gagcagcagg acgacgttat ttcaagaacg ttccacaaga gcctttaatt tgagctttgc
     2701 cgcatagaat gagtttcttt gcggataata tgcacacggg cggtcggcgt cgtcttacat
     2761 ggaacttctt gttttatgtg gaggggagct gccgctcgtt tgttgctttt tgcggatagg
     2821 tgtgcgtgat aatttacaaa gcgaaatgtc gagggcgtca accaatacag aatggttttt
     2881 gcaggctgaa cgggcgtgcg ttcatgcgaa ataaaaagga gggatatccc atggagggat
     2941 ttatacattc tacggaaagc tttggtacgg tggacggacc ggggataagg tttgttattt
     3001 tctttcaggg ctgtcctatg agatgtcttt actgtcacaa tcctgatact tggaagatag
     3061 gcacgggtac aaaaaggacg gtggaaagtc tgctggcgga atatgactca gtaaaagagt
     3121 tcatgaaaaa tggcggaatt acctgcacgg gcggcgagcc tttggtacag atagactttg
     3181 tgacggagct ttttgaaaag gctaaggaaa aggggataca cacttgtctt gacagctcgg
     3241 gcatcacttt tacgcctgac agcacggaaa agtttgaaag gctttgcaaa tgtactgacc
     3301 ttgttatgct ggatatcaag catattgacc gtgaggaaca caagaagctt acggggcatt
     3361 acaatgacaa cattcttgcg tttgcgaagt ttttgtcgga gcatggtgtg cctatatgga
     3421 tacgtcatgt tgttgtgccg ggtatcacgg acaaggaaga atatcttttc aggctgggcg
     3481 agtttatctc acagctcagg actttgaaag cgcttgacgt tctgccttat cacactatgg
     3541 gcaaggtaaa gtatgagcaa ctaggcatcg attatcctct tggtgacacg ccgcctcttg
     3601 acaaggagga cgctgttaag gcgagagatg ttatcatgag agggcttaaa gcggcgttga
     3661 aaaagagaaa ataggctttg cataagccgt aaaagcgggc gaacgctgtt cgcccctaca
     3721 gattatattg ggtcatttta atacgggcag gctttggtct gcctgttttt ttgtgtaaaa
     3781 atatattttt tgtcctgcat ttttgaaaat attctgtctg tttggataat tgacggcagg
     3841 cttaaaatat ggtaaaataa ataagtaagg aatacagcaa tagaatattg ttaggacagg
     3901 tttatattgg aaacgcacat tctctgaatg ataacgaaca acacagcgtc tgagaaaggg
     3961 ctgtaggttt gatatcataa ggaggatttt tttatgccaa agaatttgtt tcaggagata
     4021 gtttttacgg tgctgatggt gtttgtgatg gtatacgcca tgatatgcta caacattgta
     4081 ctcaacatgg gcggaatgtc aaatgccgta tttctcaagg cttttcatga gcttgtcata
     4141 atggctccga tagcttttgt tcttgacttt tttgctgtgg gaaagatagc aaagaaaaag
     4201 gctttcagca ttgttgatgt gagaaaggat aatccttttc atcttgtgct tgccatatct
     4261 gttatctctg ttatttttat gtgtccactc atgagccttg cggctacgct gctttttaag
     4321 gacgcaggca gagaagttat tgcggtatgg ttagaaacaa ctgctatgaa ctttcctatg
     4381 gctctttgct ggcagctttt ctttgcaggt ccgatagtta gattcgtctt cggacttatt
     4441 ttcagagagc gtgaacagaa aagcggggct gtatctgcag ctgcggaata aggtttttac
     4501 gagtggactt cagtctgctc gttttttatt ttacactgaa aaataaaaac taaaaaaagt
     4561 gttaaaagcc tttacaaagg caggcggttg tgttataata gtaatcgtaa aaatttgcgt
     4621 gaaaaaggag gagcttatag tgcagaaggc gtatctgatt ttggagaacg gtactatttt
     4681 cgagggtaaa agtttcggcg cagctggcga agctattggc gaaattgttt ttactacggc
     4741 tatgacaggc tatctcgaaa cacttactga ccctagttac tacggacaga tagttgttca
     4801 gacatttccg ttaattggta actatggcgt tattcctgag gattttgaaa gtgaatgtcc
     4861 tcatgttaaa gcttatattg ttagagattg gtgtcaagag cctagcaact tccgttgtga
     4921 gggtgatctt gacacttttt taaagcagca taacatcgta ggtctttacg gtattgacac
     4981 tcgtcagctc acaaaaatag ttcgtgagtc gggtgttatg aacggaaaga tcgttcctga
     5041 gggttcaagt ttcggcttga cagatcttaa gaactacacg atagttgagg ctgtaaagaa
     5101 cacgacttgc tctgaggaga aaacattcga gcctgaggga gaggtcaaga gaaaggtagt
     5161 gctttacgat tttggtgcaa agcacaacat tggaagagag cttgttaaga gaggctgcga
     5221 ggtaactgtt gttcctgcat atactaaggc agagaggata aaggagcttt cacctgacgg
     5281 cataatgctt tcaaacggcc ctggagatcc tgcggagaat gtggagataa tcgaggagct
     5341 taaaaagctt tccgagatga agatacctac gtttggaatt tgtttgggtc atcagctttt
     5401 ggcgcttgca catggtgcga tgacacacaa gctgaagtat ggtcacagag gtgcgaacca
     5461 gcctgctgag aacacagaaa cgggaagagt ttacataaca tcacagaacc atggatatgc
     5521 agtagagaac gattcactcc ctgagggtga agcaaaggtg ctttatgtaa atgccaatga
     5581 cggcacttgt gagggtatca gatatctcaa cgagcctgca ttttctgtac agttccaccc
     5641 agaggcttgt ggtggtccgc atgatactga cttcttgttt gatgagttta ttgcgctcat
     5701 tgacactaac aagaaataag ctctgatttt ttgaagtata gaaattattg atctcagttt
     5761 ctgcaacagt aggggcgaac agtgttcgcc cgtggctgac ggaatgtgtt tgatatgact
     5821 attgtagtgt tcattattcg agcggctttt atatggggcg gtcgtgatag tgaacgggcg
     5881 aacgctgttc gcccctacat tatgttttac tgaggtcgtt aagctttaac acagagggct
     5941 gctgggtgcg gttttcgaga gtgtgagaag ggataactcc tcactcctca ctcctcactt
     6001 tcagtgtttc cctaaacttt tcagaaagga ttgaatttaa gtgcctttaa ataaagatat
     6061 aaagcgagtt ctcgttatcg gctcgggtcc tatcgttata ggtcaggctg cagagtttga
     6121 ctatgcaggt acacaggctt gccgtgcgct caagcaggag ggccttgagg ttattcttat
     6181 aaactctaac cctgctacta tcatgacgga caatgctatg gctgacaaga tatacattga
     6241 gccgcttact cttgaaactg ttaagagagt tatcaaaaaa gaacgccctg acagcctgct
     6301 ttctaccctt ggcggtcaga cgggtcttac gctttctatg cagcttgcta aggagggatt
     6361 ccttgacaag tataatgtta agcttcttgg tgctaaccct gaaactatcg acaaggctga
     6421 ggacagacag atgttcaagg acactatgga gtctatcggt cagccatgta tcccttcaaa
     6481 ggtcgttaat actgttgagg acgctgtaga cttcgctaat cagcctggta tcggttatcc
     6541 tcttatcatc agaccagcct ttacacttgg aggtacaggc ggcggtatag ttaatgatga
     6601 agaagaactg aaagagatca caagaaacgg tctttatctt tcacctatca ctcaggtgct
     6661 tgttgagaaa tgtatcgcag gctggaaaga gatagagttc gaggtaatga gagacgctaa
     6721 gggcaacgtt atcactgttt gctcaatgga gaactttgac cctgttggtn nnnnnnnnnt
     6781 cacacaggcg actctatcgt tatcgctcct gctgttacgc tggctgataa ggaatatcag
     6841 atgctccgtt ctgcggcttt gaagataatc gatactctta aagtagaggg tggctgtaac
     6901 tgtcagttcg ctcttaaccc tgacagcttc gagtatgctg ttatcgaggt aaaccctaga
     6961 gtttcacgtt cttccgcact tgcttctaag gcgacaggtt atcctatcgc taaggtcgca
     7021 gctcagatag ctatcggata tactcttgac gagataaaga atgctgtaac aggcaagact
     7081 tatgcttgct ttgagcctgc ccttgactat gttgttgtta agctccctaa gtggcctttc
     7141 gacaagttcg tttatgctaa gcgtgagctt ggtacacaga tgaaggctac aggtgaggtt
     7201 atggctatcg gctctacttt tgagcaggct ataatgaagg ctgtaagagg tgctgagata
     7261 ggtcatgact gccttatctc acctaagatg cttgaccttg atgacaagac tatccatgac
     7321 agactttctg actgcacaga cgaaagactt ttcgttgtat acgaggctct cagacgtggc
     7381 gtttctgttg atgagataca cagcatctct aagatagacg agtggtttct ctacaagctc
     7441 tgcaagctta tcgacatgga aaagacactc aaaaatgact tcaacgagga aacttatctt
     7501 gaagctaaga agataggcta tactgataag gtcattgaga agataaccgg caagaagata
     7561 gaaaagcctg ttcatgctgt atttaaaatg gttgatacct gtgcggctga gtttgcggct
     7621 atgacacctt acttctactc aacatatgac aacgaggacg aggcaagcga gttcattgca
     7681 aacagaggtc atgacagaaa gactgttata gtattcggtt caggtcctat cagaataggt
     7741 cagggtatcg agttcgacta tgcgtctgtt cactgcgtat gggctttgaa agaaaaggga
     7801 tatgacgttg ttatcgtaaa caacaaccct gagacagttt caactgactt tgatactgct
     7861 gacagacttt atttcgagcc tcttaccgac gaggacgtta tgaacatcat cagagttgag
     7921 aagcctgtcg gcgttgttgt ggcattcggc ggacagactg ctatcaagct tacaaagcat
     7981 atggctgaac atggtgtaaa cattcttggt actcctcctg acgctatcga cgctgctgag
     8041 gacagagaga gatttgacga gctgcttgaa cagctgaaaa taaagagacc tcagggcttc
     8101 actgttatga cttgtgacga ggctcttgaa gttgcaaata agatacatta tcctgttctt
     8161 atgagacctt cttacgttct cggcggacag aacatgataa tcgcattcaa cgatgacgat
     8221 atcaaggaat acatggctat cattctcgac cagggaatag agaaccctgt acttatagac
     8281 aagtatctta tgggcactga gcttgagata gacgctatct gtgacggcga ggatattctt
     8341 atccctggta tcatggagca tatcgagaga acaggtatcc actcaggcga ctctattgcg
     8401 gtttatcctg catggaatgt ttctgacagc ttgataaaca gaattatcga atgctcaaag
     8461 aagcttgcta tcgcacttaa cacaaagggt cttgtaaata ttcagtatat tgcataccat
     8521 gacgagatat atgttatcga ggtaaaccca cgttcttcaa gaactatccc ttatatctct
     8581 aaggtaacgg gcgttcctat ggtagacctt gcaactaagt gtatgctggg cgaaaagctc
     8641 aaggacatgg gctacggcac tggcctttac agaaattcac catatgttgc cgttaaagtt
     8701 cctgtattct cattcgagaa gcttatcggc gttgacaacc accttggacc tgagatgaag
     8761 tcaacaggtg aggtgcttgc agtttctaat tctcttgaag agtcactgta taagggtctt
     8821 actgctgcgg gctacaagct tggcaagaag ggcggagtat tcatcactgt cagagattct
     8881 gacaagggcg agataccaca gacagctaag atgttcgctg accttggatt taagatatac
     8941 gctacaaacg gcactgctaa ggtgcttcac gagcacggca ttgacgctga ggtagtggca
     9001 aagatacacg agaacgagga caacaacact ctcacactta tcgagagcgg aaagatagct
     9061 tatgttatct caacttcttc aaagggaaga atacctacaa gagattctgt taagataaga
     9121 agaaagactg ttgagtggaa cattccttgt ctgacttcta tcgacactgc aaacgctatt
     9181 gcggcatcta tcagaagtaa atacactgag tcaactactg agatagttga tatcaacaac
     9241 atgagaactg aaaagcagac atttgagttc actaagatgc agggctgcgg caatgactac
     9301 atctacttca actgctttga ccagagaata gacaatcctg agggcctggc actcaatctg
     9361 tctgacagac acttcggtat cggcggtgac ggtgttatcc ttatctgccg ttcaaaggtc
     9421 gcagacgcta agatgagaat gttcaatctt gacggttctg aaggcaagat gtgcggaaac
     9481 ggtatcagat gcgttgctaa gttcatgaga gataacggca ttgttgacaa ggacgaaatg
     9541 actatcgaaa cactttcggg tatcatgaca gtttcactta tccgtcacta cggtgaggta
     9601 agcggtgcta ctgtaaacat gggcaaggct attcttgctc ctcaccttgt tcctgttgaa
     9661 cttgagcctg acgagaacgg cagggtagtt gacagaaagg tgaacattgc aggaaatgac
     9721 tacaacatca cctgcgtttc aatgggcaac ccacacgctg ttgtattcat gaacaatgtt
     9781 gacagccttg acattgacaa ggtaggtcct gagttcgagc atgacaagat attccctgag
     9841 agagtgaacg ctgagtttat caaggtgatc gatgaccaca cactcaagat gagagtatgg
     9901 gagagaggct ccggcgaaac ttgggcttgc ggcaccggtg cttgtgcagc agcagttgcg
     9961 gctgttctta acggattctg caaaaaggac gaggagatat ctgttatact taagggaggc
    10021 actcttaaga taagatacac agacgaggct gtatatctta caggcgacgc tgtaacagta
    10081 ttctcaggtc acatcgtaat gtaaatttcg cagcggacac tgcgttaaaa aagcggcagc
    10141 cgcagagcgt tttgaatgtg ctttgcggct gtgtgcgttt tataagtttg acccgtgaaa
    10201 cttcgggtcg caaaaatgaa aaggagttgg aattatgcca agtataaacg aaaattttct
    10261 caagctggaa aagaattatc tttttataaa cattgccaag aaggtaaagg ccttcactga
    10321 ggcacaccct gaggctgaca ttatcagaat gggtatcggc gacgttactc agcctatcgc
    10381 aaagtgcgtt gttgaggcaa tgaaaaaggg cgctgacgaa atgggcgtca aggaaacatt
    10441 caagggctat gaggacagcg gtgcaggata tgacttcctg aaagaggctg ttgcaggcta
    10501 ctacaagagc ttcggtgttg acattcctgc tgacgatatc aggataaacg acggtgcaaa
    10561 gtctgactgc ggaaacattg ttgacatttt cggtgacgac aatgtagtgc ttatcacaga
    10621 ccctgcatat cctgtttatg ttgacacgaa ccttatgaac ggcagaaagg taatttatgc
    10681 tgattcaaac gaggagaaca actttgcggc tatgcctgac gagaacgtaa agtgcgacat
    10741 tatttatctt tgctcaccta acaatccaac gggttctgtt tacactaagg agcagcttaa
    10801 agtttggata gactatgcaa agaagaacaa cgctatcatc atttacgatt ctgcatacga
    10861 ggcattcatt acagaaaagg acgtaccaag aagtattttt gctgttgagg gtgcaaagga
    10921 atgtgctatc gagatgtgct cactttcaaa gacagcaggc tttacaggaa tgagatgtgg
    10981 atatacagtt atcccacacg agcttgttgt taaggcatct gacggcagtg aagtttctat
    11041 cgctcagctt tggggcagac gtgaaggctc aaagttcaac ggtgtatcat atcctgttca
    11101 gtgtgctgct gcggctgtat tcactgagga gggtcagaaa cagacaagag caaacattga
    11161 atactatcag aacaacgcaa aggtaatggc ttccacactt gacgagcttg gtatcaagta
    11221 cacaggcggc aagaactcgc catacatctg gctgaaatgc cctaacaaca tgggttcttg
    11281 ggagttcttt gattatcttc ttgagaacgc aaatgttgtt ggcacacctg gtgcaggttt
    11341 tggcaagaac ggcgaaggct ggttcagact tactgctttt ggtgacaagg acaggactgt
    11401 tgaggctatg gagagaataa agaagctgct gactaagtag tgaggagtta ggagttagga
    11461 gtgaggaatt attcttgctt ttactctgat attctcggat aggttttata tgattttaaa
    11521 agggcggttt tgaccgctct tttttttgtt cgcaaattct accgccgcaa aatcacttcg
    11581 ctcttcgcgg attgtacttt gtacaaagat atagtaaaag cggtgcagag ttttttactc
    11641 cgacaccgct ttgttatttg ctttagtttg tgtttcttct acttggtagt ggctctttgc
    11701 taggtttggc tatgccattc ctcactcctc actcctcact tagcctacac ctttattctc
    11761 cgatactctc ccggagtcat attttcatac cgtttgaata ccacggagaa ataagatccg
    11821 tcctgaaagc ccgtttcatc tgatatctcg cctatggtct tgttggaata cacaagcaga
    11881 tcctttgcgt gctgtactct tacttgcgca agatactcca taggtctgag ctgaaaattt
    11941 ttcttaaaga gtctgcataa gtgctgctcg gatactcctg cgatttttgc aagctcgtta
    12001 agctttattt gctccggata atgctcttca atatactgca atacgtctgc caaggcaaca
    12061 gattgtgcgg tataaaatga cgagagcctg ttatcagcta ttttacgata ttctataaga
    12121 aagtcgtaaa gctgtgctga agcgtaatga ttgccataaa gcttatcact tttcagagta
    12181 taatagatgc gtgttatgat acggcgtaat ctgtccatat ctaccccttg aatggcggtg
    12241 aacttattaa gcttccactg ctccaagagg ggaatgacct gtgagccaga aaaggctatc
    12301 caatccagat accacgcatc gcttagcgca tgatactcat gtggacagaa tggggggata
    12361 tagaaaacag tatcgggcgg tatttttgtg gtttctccgt ttattatgac ctcgccctcc
    12421 cctgagcgtg acaaaaatat ttgtgggtac tcgtagccat ctgcacgttt tattggaaac
    12481 tgccagcttt caagtcccag acctaccaca taaaagggca ggtttttttc gtcgcctatg
    12541 acgggatata tctgtttgta cattattctg cacccccaat ttacatatct ttataatacc
    12601 actgggtttg gggtttgtca agataaaact attcttttgc tgagctttta aagaaaagcg
    12661 aaaagataaa ggcgaatgtc atggctgcag ttatgcctgc ggcggaggcg gtaagtcccc
    12721 ctgtgaaaat gcctaggaag cctattttat ctatctgttc acggacaccg ttatagagaa
    12781 gatagccgaa gcccgtgaga ggtacagttg cgccacagcc agcgaggtct ataagtggct
    12841 tataaagtcc cagcgcaccc agaagaacgc cgcctgtgac gtatgccacc agtattcgtg
    12901 cgggggtcag ttttgtgaag tccataagca cctgtcctat ggcacaaaaa agacctccta
    12961 taagaaaagc ccatagatag tccattagtt tgctcctttc atatcttttt cccctgacag
    13021 ccacacgaga tgtgagatag aggggataga ttccccttgc tgatttactg ttggtgacat
    13081 tagcgcaccc gtggcagcaa agaggatatt ttttatttca cctgcacgga gcttcgggag
    13141 aaaatagccg cacagaacgc ttcctgcaca cccgcagccg cttccccctg cgtggatatc
    13201 ctgctcgtca aagtcataga gcatcatgcc gcaatcttta tgattgtcac ggatatttat
    13261 attttcacgc tcgaaaagct cacagagaat acggcttccg actttgccaa ggtctcctgt
    13321 gacgatgtaa tcaaagtctg aaggggtcat gcctgtgtcg gtgaaaaagg tgcttatagt
    13381 ggaaaatgct gcaggagcca tggctgcacc catgttgttg gcgtcgctta cgcccaagtc
    13441 aactattttt ccgatagtac agcccctgat gtaggggggc ttgtccgagg gggcaaccac
    13501 caatgagcct gaggctgtgg ctgtccactg agatgtggga gtacgctgtc caccgtagtt
    13561 gaggggaaaa cggtactgtc tttcagcggt gcagaagtgg gaagatgtga ccgccattgc
    13621 tttttttgcg aggtcgttgt caacgaaaag agaggcgagg agaagtccct ccgccatggt
    13681 ggagcaggct ccgtaaatgc cgagaaaggg catttcaaag gctctcacgc cataggtagt
    13741 gccgacacac tgatttagca ggtcaccgcc gaaaagcacg tctatatcct cgggggagag
    13801 ggcggcttta tccaaagcgt gaagcactgc ctgcttttga agctgacttt cgcccttttc
    13861 aaagctatct gtggataaat agggatcgtc atttattttg tcaaaatagt cgcccaaagg
    13921 cccttcgttt tctttcttgc cgcctatggc tgcgaaagag attacagagg gtgaggtttt
    13981 cataagctta gtggctttca ttttatcact cctattaatg aatatcgtca tttgatgtta
    14041 ttttctacat tgcagatgaa aatatgcaaa aaaagtttgg cgttttaaac gtaaacttaa
    14101 aactatctta aaataagctt gttttgatct caaaatcgtt gacatattca caaaaaaaag
    14161 atataattaa atagtcgatt tattgttgat tatgtagtgt tttgttaagt ggtatgtgtg
    14221 aagggaggat atgtcccgtc gggggcatag ttttagatga agaaaaatga agataagccg
    14281 gtggtcataa agcttgaccg aaaaaaaata aagcagtcgg ctaaaggcag ggtgaagcga
    14341 aattatatcg cctgcatagc agtgtgcttt ataatggtat ttattgcagg agaatatggt
    14401 tcgaccacgc agaatgtgtc aagctatgat aattctcacg ttgctgatct taaatatgac
    14461 gccgaagata aggaagagat cattgaggat atggttaaaa atggtcttat ggcagagcag
    14521 gtcagtgaca agtggcagat agataatcct gacactgtaa aaaagtggtt ggacgcttat
    14581 aaaaaatacg gagttgacgg gctaaattca aaggaagtca cgttctttgg ttcatcaaac
    14641 gacacttcta atctggaagc actggcagac gctttttctt ttagccgaaa tgcaaaggaa
    14701 aaggcacaga cctatcttaa tgacaggctc aataatttta cagcctctgc ttcggtttat
    14761 tttgacagtg caacaagctc taattcctat cagttttcat tgcttacggc gataatgaat
    14821 gttttaggaa aaaaatccac atgggatact atagtttcat tcgtgtattt tattttccag
    14881 ttgatgttca ccatattcat agtgaatgtg ctttgggtat gtgagagaag atttttcctt
    14941 gaaaatcaca catacaagaa aactaagata ggcagactag gctttctttt ccgcgagaga
    15001 acgcttcacc ctgcaaggac tatgtttgta actagcatat atcagacctt atgggcgttt
    15061 actatcgtgg gatatcctat aaaacattat gcctattcaa tggtaccgtt catatgtgcg
    15121 gaaaacccta acatcaagac gagaaaggca ataaagcttt ctatcgccat gactagggga
    15181 tacaagtggc agcttttcaa ggtggatctt tctatgatag gctggacact gctttcgagc
    15241 atatcgttcg gtgcggtggg agtattctgg tctaacccat atacaacggc gatagacgca
    15301 gaggtgtatt tacagctaag gcgtgaggct atcaagaaca agatagaggg ctatgaggag
    15361 cttaatgaca agctgcttga ccttgacctt ctggaagagc ttatggctga ggaagctgca
    15421 caaaagggtg aaaatcctga tattgttaga agcataccga tatgcacgat aaaagtgcct
    15481 gaaaataatg agaatggggg cggtgaataa tggagagaac gctgacgctt gacgatctgc
    15541 gggaatttga gcaataccca ggtcttgcgg tagacagaaa ggcgcatttc agccgatttg
    15601 ccaacaagac tatagttcac cgtgactatc acagaaacta tacactcatg acgtatatac
    15661 tgctgttttt catattcgca tttataggct ggatatggga agtgggcata cacatcgttg
    15721 aggacggagt tttcgtcaat cgaggaatca tgtttggccc gtggcttcct atatatggct
    15781 ttggcggtgt gtgcggaatc atattgctga agaggtttat agaccatcat gtggcgactt
    15841 ttttccttgt tgtggggtta tgctcggtgg tcgaatatgc aacgagctgg tatcttgaaa
    15901 cgttcaagca catgaaatat tgggattaca cgggatattt ctgcaacatc aatggcagga
    15961 tatgtcttga gggagcgttg atatttggct ttgcaggctg tgcaggaata tacatcatgg
    16021 caccgttttt tgatgatctt ttgaagaaga taccattcaa ggcaaggttt gcggcgtgtg
    16081 tgatacttgt tgtattattc tgttgtgacg ctgtttactc aaaggctaat cctaatcagg
    16141 gcaagggcat aactgattat gcggcttaca atctgccttt tcacagggtc gtggttagtg
    16201 aggagctagg agttaggagt gaggaattat tattgctaca ctctgatatt ctcggatagg
    16261 tttgatatga tttttagaag ggcggttttt gaccgccttt tttctgtgtg gaaattctgt
    16321 aggggctggc gtcctagatg tcccaaattt ggtcgcctgc ttaatcctgt gttatccgca
    16381 caacattcga cgtattgttt caaaatgctt aacggcgtaa catttgcaac acggtacgtc
    16441 tacacaaaat tgtgataaag caaaaccttg acactctccg aacgggacgt cgagggcgcc
    16501 gtcccctaca ttttgttcgc aagttctatt gctgcaaaat cgctggcgct ctttgcgggg
    16561 ggagtttgta aaatgagata gtaaaagcgg tgcagagttt ccactccgac accgctttat
    16621 cttttgttct ttatttagtg cttgtgattt actcggtttg gctatgccat tcctcactcc
    16681 tcattcctca tttaactaga gctttaatcc aaagcttatt gataatgccg aatttactgc
    16741 ggacatggag cttacgtcaa gtgttcccat tttctccttt agccttttct tgtccaatgt
    16801 acggatctgt tccaacaata ccacggaatc tttcgctagt ccgcaggtgc caccgccaac
    16861 ttctatatgt gtcggcagtt tcgctttatc aaggcggctc gttattgccg ctgctatcac
    16921 tgtgggtgaa tgtttattgc ctatgtcatt ttgtactatc agcaccggtc gcataccgcc
    16981 ttgctctgag cctaccactg ggctgagatc tgcataatat atatcgcccc tgtgtacagt
    17041 cacttgtaaa cactcctatt ctatgggctt tcgccttgat ttgcaggctt ttgctcctgc
    17101 ttgttcctat tattgcctta ttatcggggt attataccaa agtatctatt ttgttacaag
    17161 gtgcagggct gttttatttt atgcggcagc agtgctttgg gtgaaaagtc agtattggtt
    17221 gacttttcac ggtcaaagtg atatacttaa catagtaaat aaaggcgtaa atgccttgaa
    17281 aggaatgatt ctatgagcga aggctgcaca catgattgcg aaaactgtgc ttcaaaatgc
    17341 tctgataaaa agagcttgaa agaggagctt aatgctgcga gctctgtgaa aaaggtatat
    17401 gcagtaatga gtggaaaggg tggcgtaggc aagtctttgg tgacaagtat gctcgcagta
    17461 acggcacaaa gacgtggttt taagaccgct gtgctggacg ctgacataac aggcccgtct
    17521 ataccgacag cgtttaatat ccacgagaga gctaatacca atatgtatgg cattatccct
    17581 gcggtgagca agaccggtat cgagataatg agccttaatc tgcttcttga aaatgaaact
    17641 gaccctgttg tatggcgtgg ccctgttatc gcaggtgcag tgaagcagtt ttggacagat
    17701 gtggcatgga acgacgttga cgtgatgttt atagatatgc cgcctggaac aggcgacgtg
    17761 ccactgacag tttttcaatc cattaaggtt gacggcatag ttatcgtcac ttcgccacag
    17821 gaattggttt ctatgatagt cgagaaagcg gtgcatatgg cggatatgat gaaagtaccg
    17881 gtgcttggaa tagtggagaa tatgtcatat tttgagtgtc ctgattgtgg aaagaagcat
    17941 aatatctatg gtgagagcca tgtggacgaa gcggcagtga agtttggtat cgacgctgtg
    18001 gcgaaaatgc ctatagaccc gaagtttgcg aaagcttgcg ataacggtga gatagaagaa
    18061 gtcgagggcg gctggctgga tacgatcgcg gataaacttc tcggttaaag tgaggagtga
    18121 ggagtgagga atggcatagc caaccgaaca aatcacaagc ataaagtaaa agtcaacacc
    18181 gagcgaagcg agcaaaaggt ccaggggcac gcccctggtc agggtcaagg g
//
LOCUS       DAWEMR010000032        18184 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1275_length_18184_cov_13.197308,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000032 DAWEMR010000000
VERSION     DAWEMR010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18184)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18184)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18184
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1275_length_18184_cov_13.197308"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4319..4328
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4896 a         4120 c         3661 g         5497 t
ORIGIN      
        1 gaaaggtaac actccaataa aagatgttat gtatcaattt cactataatt atattaacac
       61 acctacattt atttgtcaac agcatacata aaatttgttt ttcaaatctg tttttttaac
      121 aaataaactt attttgtttt gtataaaacc tacaattatc agcctgaaaa gcacaaaaaa
      181 accgtggaaa cactgctgtt cccacggctt tcggtatgtt aagagagtat ctgtatagct
      241 ttattacttt gtcataagct cacaaacaag gtttgcaaac tcaacagggt tgtcgataga
      301 cattccctcg ataagcaaag cctgatcgta aagtagcttt gcatagtctt tcagcttgtc
      361 cttatcgtta gcatagaggt ctttcagctt ctcaaagata gggtggtttg gatttatctc
      421 cagtgccttt tcagccttta ccttattgcc gtcattctga ggcatagcgt tgagtacctt
      481 ctccatttcg atagttatgc tgccgtctga tgaaagacaa acagggtgag ttttaagctt
      541 ctttgaaaga cggacagtct ttaccttgtc agcgatagct tcctgcatga aagtgaacat
      601 atccttgttc tcttcgtcaa gctttttagc ctcttccttt tcttcctctg tgtcaaggtc
      661 gagatcagca tcgcttatgg acttgaaagg cttgccgtca tagtttatca tcaccttgat
      721 agcgaactcg tcaacgtcct gtgtaaagta aagaacttca tagcccttat ccttgatctt
      781 ttcaagctga gggagcattt cgatcctttc ctttgtttcg ccgcaggcat aatagatctc
      841 tttctgatct tctttcattc ttgaaacata ctctttgagt gtaacgttct tgccgtcaaa
      901 tgaggacggg aacataagga gatctttcag agtttcgttt gcagcgccgt atgactgata
      961 aatgccaaac ttaaactgaa gcccgaatac gctgtagaac ttctcatact tctcacggtc
     1021 gtttttcaga agctttgcaa gctcgttctt tatggacttt tcaaggttct ttgcgattat
     1081 cttgagctgt ctgtcctgct gtaaaagctc acgggagatg ttcagtgaga gatcctcaga
     1141 gtcaacaacg ccctttacaa agctgaacca gtcaggaagc aggtctgcac acttttccat
     1201 tatcattaca ccgcttgaat aaagctggag tcccttttca tagtcctttg agtaatagtc
     1261 aaaaggtgca cgggcaggta tataaagcag tgcgtcatat gttgcaacgc cctcgttctt
     1321 tgaatggata tgtgcaacag gtggctcata gtcgccgaac ttctccatat agaagttgtt
     1381 gtaatcctcg tcctttagct cactcttgtt tttcttccag ataggggtca tgctgttaag
     1441 agtttcaata gccttgtgta cctcaggctc tttgccctcc tcattataat gagtatgctc
     1501 gacttccatt ctgataggga atctgatgta gtcagaatat ttctttacaa gtgactgtat
     1561 ctgatactga tcaagatatg ttgaatactt ctcgtcatca gtatcgtcct tgagtttaag
     1621 tgtgatagtt gtgccgacgc cgtcaggctt gtcacactct tctattgtgt agccctcaac
     1681 accgtcagat gtccacttgt aagcctgatc gctgccaaaa gcctttgtta cgacggtaac
     1741 ttcctttgct accataaaag tagaatagaa gcctacgccg aactgaccga tgatatcaac
     1801 atcgtctcca aggtcattgt ctttcttaaa agcaaacgag cctgagtttg cgatagtacc
     1861 aaggttgttt tcaagatcct cctcggtcat accgatgccg ttatcagaaa ctgtaagcgt
     1921 tctgttttcc ttgtcgatag cgatattgat agcgaagtcg ctctttttta ttcccacaga
     1981 agtgtctgtc agggacttga aatacagctt atcaatagcg tcgcttgcat ttgagattat
     2041 ctctctgaga aaaatctcct tatgagtgta gatcgaattg atcatcatat caagcagtct
     2101 tttggattct gccttaaatt cttttgtctg cataaaacct cgacctttcc ttattttttt
     2161 acttttcagt attgttagca atataattag cactctatct atttgagtgc taattatatt
     2221 atacaacaag gttgataaaa tgcaatagtt atcacaaaat ttctcttatt tatttacaaa
     2281 tccataacat ttcatcacat acctgacaaa ataccatttg caaaagcacc atgttgtgtg
     2341 ttataatata tagacaagac cgaacaaggg aggaataata tttgactacc aaaaagtctg
     2401 tttctctgct gacccctgaa aaacagcagg aaaagaaact tcttactata gattgcgtga
     2461 aaatttcact tctgctcatt ttatacaacc tgctaaacag gatattcgca gtgttattct
     2521 tttacgttgc atacacttac cactcaggaa aattcactct tagctactcg gcagttctca
     2581 gctatctgaa aaagcagacc gcttttataa gctcaactac tttttccatg acggcaaatt
     2641 tgtttatcac tcttatgagc gtgcttgttt ttgggatagt ttttgctata cttttccgaa
     2701 aacgagaatc aagcttcata aagccgaaaa aaggcacggc agtaaaaggg ctaaaatggt
     2761 tttcagcctg ctttgtgata aacattctgc tgtcacagct catatctatc ctcacgggta
     2821 tacttggtca aaacggagtg acagttccca caagcgattt ttccattcat cagccctctg
     2881 cccttgcagt aatagtgcag ttctcatacg ttgtgataat agcccctatc gtggaggaat
     2941 ttatctaccg tggagcgatc ctcacggctc taagcccata cggaaggggt gcggcggtgc
     3001 ttttctcggc acttgcattc ggacttatgc acggaaatat ccctcaggcg gcttcggcat
     3061 tcggcacagg acttgtttac gctattatcg ccgcaaagtg cggctcgata ctgccaacag
     3121 ttataataca ctgtctaaac aatctgcttg caaacttctc gtccatatct gatgcaatag
     3181 ggcttgaaca ctatgagacc ttcatgagcg ttgctgagat agtcataggt ctggtgggct
     3241 ttatggtgct tttcacaagc cttaaaaagc ttctcattga tgacagcgaa aacgtgctta
     3301 caccaaaaga gtgccgcaga tatgttttca caaaccctgc cacaatagtc tatatgctct
     3361 gccttatcat accgatagta aagagccttg tccaggcaaa cagctaaaaa tcacaaacaa
     3421 aaaacgggtg actgccaatt atttgacagc cgcccgatat ttttatatcc aaagagccta
     3481 ttaataagcc ttgccccaaa gcaccatatg ctttgctggc tttccacagc atacgcaggt
     3541 atctgctatc tcctcctgct caaatgggat acatcttgag cctactcctg tgagttcctt
     3601 taccttatcc tcgcaagctt catcgccgca ccacattgcc ttaacaaagc cgtcgccctt
     3661 ttcctcaaga gcctttgtta tctcgtcaag tgtcttgcaa acataggtgc gtctttctct
     3721 gttttcaagt gcctttgcat aaagaccatc atgaacagcc tgaagtcttt catttactgt
     3781 ttcaacaagc tcaccaagct tgcagaatac cttttctctg ttgtgtcttg taacaagcac
     3841 acactgatcg ttctcaatat cctttggacc aatttcaagt cttacaggaa cgcccttcat
     3901 ctcatactgt gcaaacttcc agccaggtga ctgctctgaa tcgtcgagct taactcttat
     3961 gcctgcattt ttaagcgtgt tgtaaagctc gttagccttg tcaagaacgc cctctttgtg
     4021 ctgtgctata ggtacgataa ctacctgaac aggtgcaaca gcaggtggca gaacaagtcc
     4081 gttatcgtcg ccgtgtgtca ttataactgc accgataagc cttgtcgtaa cgccccatga
     4141 tgtctggaat gggtgtgcaa gcttattatc tcttccagta taagtgatat cgaaagcctt
     4201 tgcaaagcca tcgccaaagt aatgcgaagt gcctgcctgc aaagccttgc cgtcgtgcat
     4261 aagagcctcg atagcgtatg ttgcttctgc acctgcgaac ttgtcgctct ctgtctttnn
     4321 nnnnnnnntg ccctttacga ccggcatagc aagctgctgc tcgcagaaat cagcgtatac
     4381 gttgagcatt ctctctgtct cctcgatagc ctcctcaggt gtctcgtgga tagtatgacc
     4441 ctcctgccac aggaactctc ttgaacgaag aaatggtctt gttgtttttt cccaacgtac
     4501 aacagatacc cactgattgt aaagcttagg aagatctctg taggaatgaa ctatattagc
     4561 ataatgctcg cagaaaagtg tttcagacgt tggccttacg caaagtctgt cttcaagctt
     4621 ttcacttccg ccgtatgtta cccaagctac ctcaggtgca aagccctcaa catggtcttt
     4681 ctccttctga agcaggctct cagggataaa cataggcatg caaacatttt catgaccaag
     4741 ctccttgaac tttgtgtcaa gtatatgttg gatattctcc catattgcgt agccgtaagg
     4801 tcttattacc atacagccct ttacgcttgt gtatgaaaca agctcagcct tcatacatat
     4861 atcagtatac cactttgcga aatcctcgtc catggaggtt atcgcatcaa ccattttctt
     4921 atccttagcc atttataaaa cctctttctt atatcattca ggcattgtca ttattcttgt
     4981 tgccgtcgtc ttttttaaca gcttcactgc tgacattctt ctttttcttt ttaaaattaa
     5041 gagcatagat atcctcgtta tatatcttat agttgggatc ccagccgtca atgtgggact
     5101 tatcaaatat gccctgtacc ttatagttct caggatcatt ccctgtattc tcaccgtcca
     5161 ccctttcggg atcgtccttt tcggcaagct cagggtgacg tttttcaata aatcgtgcta
     5221 tttttggagt gataaggcag gcaatgacaa taaaggtcat tattacgaca cagcctgctg
     5281 ccagcttcaa cagcaatagc agtaattctt tattcagaca agtcacctct tttcgcaaac
     5341 ataaagttat tatagcacaa ttattatcca aattcaagag gtgaaatatg aatttaccgc
     5401 catttctgac ctttagcacg aaaaaaggcg gaacaaagtc cgccttaaag cattttcttt
     5461 cgtttgaaaa agattataca cactatcact actattatac taagggctat aacacccgga
     5521 tacccttctt taagttcggg catattctga aagttcatgc cataccagcc cgtgataagg
     5581 gtcagaggga aaaatatagt ggatatgacc gtaagaaact ccatattctt gttctgctga
     5641 gcattgacat gggactgata agcgtctcgc acctgcgttg catattcaag aagatgtaca
     5701 gttctgccca tgagcctgtc ggcacggtct gagatagtgc cgaaatattt tttctgcttt
     5761 ttctcaaaga agccgttctc gtcctcttca agctctctgc ccagatccat tatttcatca
     5821 tagtagcttc tcaaaataag aagctctttt ctgataggcg ttatggtttc aagaaaatcg
     5881 tccgtcttgc catcgtttac gttctcctca agctccataa gcttttgctc atgttccgca
     5941 agaagttcgg tgtcacggag cataaattcg ctgaaaaaat tgtagataaa atgctccctg
     6001 ctctctccct gattatgcct gcttctctgt atgcgttgaa ctaacctgag tgaaaactcc
     6061 tcatcgtcta ttatcaccat gtttttgttg tcgataaaaa actgtatcct gtggcggctt
     6121 ccctttatgt caataagctt ggggatagaa agcgtgcctg ccagacagaa aggctgtgat
     6181 tctatcttac agaacacaac gtcacgcagg gatatcacgc cctcgtaggt tatgttcgcc
     6241 gttctgaggg tttcctctgc gtgagcagag tcggtaataa acacagccga gcaagtttgc
     6301 tcgcttagct gtgccggtgt tatttcttca agtgaaccct tcagggcaaa tattgtcatt
     6361 tatcctcgac ctccgccttt gaagctcttt cataggcaag gtcatagaaa agttcctgag
     6421 aattgagtgg ctcttttccg tctgaaacaa gggtatattc cccgtcagaa tcaagctctc
     6481 ttgcctgaca attatctgca agcattttgt cgaacatcat gcttatctga gacttgatat
     6541 cctcgtcaag aataggtgtg gcgacctcaa cacgtctgag ggtatttctt gtcataaagt
     6601 ctgcggaagc aatataaatc tttctttcct cgcccttgcc aaacatatag atacgggaat
     6661 gttcaagaaa acgtcctaca atgcttatta tcctgatgtt gtcggtttcg ccctcaacgc
     6721 ctgggataag acagcatatg cctcttacca caaggtctat cttaacgcct gctcttgaag
     6781 cttcgataaa tctgtcgatt atcttcttat cagtgagaga atttatcttc acgccaatat
     6841 agccctctcc ccctgattgt gctatggcta tctgctcgtc tatcatatca agcaccttgt
     6901 tttgcagaca attcggtgca acaagaagct tgtcaacgct ctttacagta tcgtcagaag
     6961 caagagcatt gaacacgtct atagcgtcct ttgcaatgtc cttatcgcag gtcataacag
     7021 acaggtcggt ataaagtctt gaagttttct cgttgtagtt tccagtgcct atctgagtat
     7081 aatactcggt ctttgtgcct gaacgctttg tgataaggca aagctttgaa tgtaccttat
     7141 agccctccag accatagata acagtacagc cagccttttc aagtcggcgt gaccactcga
     7201 tattattttc ctcatcaaat ctcgccttaa gctccaccaa tacaagaacg tccttgccgt
     7261 tctctgcggc ttcacaaagt gcttcgacca cctcactgtg ctttgccaca cgatacaatg
     7321 tcatttttat agacaccaca tttttatcgt cagccgcctc acggagcata ttgataaaag
     7381 gctttatgct atcaaaagga taagaaagca gtctgtcttt cttttttatc tgcggcagga
     7441 tcggcttgtt atcgtcaaag gcagtaaacc tctgcggaac tctcttttta tagaaaagct
     7501 cggttttctg tctgagtata tcctgtatct ggaacaaaaa tgacagatca agaggagtgc
     7561 tgtttctgaa aacagatttt ttattcactt caagctgttt gcagagcagg tcaactatgc
     7621 cgctgtcaag ctgtcttgaa agctccagtc gtacaggcgc aaggcgcttt cttttcttga
     7681 taaggtcagc catgaactca cggtaatcaa gatcctcgtc ataaagagcg tcagcgtcga
     7741 tatcagcatt tcttgtaact ctgataaggg acttcgactt gacggtgtag cccttgaaaa
     7801 ttttcggaac atagtggagg ataagctcct cagaaagcat atacttacct gttgaaccga
     7861 cctttataag ccgtggaaag acctcgctcc cgcaggagat aacacccagc ttttccttgg
     7921 aatttttgct ttcaaggaca actgcggcat agatatcctt atttttaagg aaagggaaag
     7981 gctgtctttt gcccaccacc ataggcgaca ggagtggagc tatctgatta ataaaatact
     8041 gttccaactg agaattttcc tccgctgtaa gcttggaaaa atcaacaaga gcaacgccct
     8101 gctcggctat tttcttcata aggtcagcgt agatcttatt tttacgcttg ttaagctcgc
     8161 ccactctagc gataacggct ttgagctgtt ccttagcggt catatttgtt ttattttccc
     8221 tgctgtcagg gtctacctcc atttggtcag tgagcgaacc cacccggacc ataaagaact
     8281 cgtccagatt gctctgatag atagaagcaa aggaaagtct ttcgcaaaga ggcacgcact
     8341 cacgctcagc ctcctcaagg actctttcat taaatttcag ccatgacagc tctctattca
     8401 taaacacatt tttcattgtg ccatactcct tttattatga tatcaaaaca tctatactta
     8461 tttaatacat tatatcacaa aacgtcaaac tgttaaagag gtttaagcaa aattcatgta
     8521 aaatattctt attatccact tactatattg tagtcgcaca caaaaattat gcaaaatgac
     8581 gcatttgctt tttagtgaaa gcataatact gtgcaaaaaa gcaaaaatca aaaaagtcca
     8641 atttttttgt gcaaaaaagg ttgacaacgg ggcataaata gtgtataata atcaagtcgc
     8701 tatgagaaac gcatatggcg gtatagctca gttggctaga gcacttggtt catacccagg
     8761 gtgtcaccgg ttcaaatcca gttaccgcta ccacatttct catagcgaaa aatcggcccg
     8821 ttggtcaaga ggttaagacg ccgccctttc acggcggaat catgggttca attcccgtac
     8881 gggtcaccaa atcatatgct cggaaacagc gtaaatgcgt tgtttctgag tttttatttt
     8941 tccctaaaat ccactcaacc acttatctgc cacttatttt gaaaataacc gctttcgact
     9001 acctcttggc tgtgttctat gtaccccgca aggcttgcgc cctgcggtta ttcttatgcc
     9061 ccgaaaactt gtccgaactg ttcttcttcc tgctcgtcct catcttcgta ggtgccctct
     9121 gtgctgtcct cggagtgctt ttcttctttc gctttaacct cgttcgtgaa gcttagagcg
     9181 ttgcttatcg catcgcagtt gcgtgctctc gcctcttgga acatatggga atataaattt
     9241 aaggtcgttc caacaacgct atgtcccata tctgcggaca ccgctacaac gtccacaccc
     9301 tcaaaaatca atagcgaggc atggaggtga cgtagagagt gaatatttcg aaacggtatt
     9361 ccgatgcgct cgcagtagcc ctgaagccac tcaaagggag tctgcggatt cataggtgcg
     9421 ccgttccatt tggtgaacag cctgtcgtgg tcctcccatt ttgtaccgag ctgtttcgct
     9481 tccttgtcct gctctgtctt gaactgcttt agcagattca ttatagacgg tggaaacttt
     9541 gacacacgct tggactttcg cgtcttggtg gtgtcggtgt atatgccctt ttcctttgta
     9601 tactgagatg ttctgcggac atggatcagg ctgttctcaa agtcgatatc tttccattcc
     9661 aatcccagca tttcgctgcg cctgaagccg ctgtatatcg aaagcagaag gtacacctgg
     9721 tatttcatag gctcgtccct taaatgttcg tagagtgttt ttacctcgtc cagcgtatat
     9781 atctgctttt cctcttgctc ggattttgga acaaccaccc gtctgcaagg gttgtcagtc
     9841 agcattccca tacggatagc gtagctgaac acatcgctga taaggctgag atgatgcacc
     9901 gctgttttgc gtgacagcgg ctttccgttg ttcatgttct tgccgttgat aagcaagtca
     9961 gtcacaaatt tctgaatatc tcgactcgtt attttgtcaa gtcttttatg accaagtgct
    10021 gggtatatgc gtttggtgag ttgtttcatt ctcgaatatg atgtggaacg cagattgacc
    10081 tttgcatatt cttcaaacca ctgttcggca aatgtctcaa acttgactgc cgcagttatc
    10141 tgtcctcgct tgcagtcctc ctcgaaaagt attgcttgac gctgcacctc cttttcgatc
    10201 tgccgaggtg tcataccagg ctcaggtttg tagctcttgt actggttgcg ctgcttaccg
    10261 tctacgccgt agccgcatga tacctttatt tcgtaagtcc cattgggacg ctttctaata
    10321 gtcgccatat atattaaaac ctccgtttca aaaccatggc gcatttctac cgacaacatt
    10381 ttaccacatc tcggtggaaa agtccagaga aatgcgccgc cgtatacttg tatctttcct
    10441 atgagctaat ttcttacgat gtttttccac ggagatagtt cagaaaaata tctttgttaa
    10501 tgagatactt tctgccggcc attatgtgcg gaatttgatc gttgatgcac atctgacgta
    10561 ctctgtactc ggtcatgccg tttacaagag cagccgcctc cttgatcgta agcatggtga
    10621 tctttgtttc gtttttgttt tctgccattg tattatcgct ctccttgttg ttttggtaat
    10681 tctgcttatg gtatgtgtga tgttcatttt tacagctgtc ctttcatagt gttttcaagg
    10741 gtacagtgaa atgttgtacc cttggtatgt cggaaatata ttacgtgttc tcatgtcagt
    10801 aaagctgaca tacagcaacg ttttgttaca caacttaata tgaggtaagg attcgtccac
    10861 tcatcggaga cctcgtcacg ttccgttact ctatcgatgc ttgttcagat tattcatcat
    10921 cggctatacc ctcatcaaag tttcttgtta tcagatcatc aagattgatt ttccctcttg
    10981 tgcgtctgac attgttgacc gcctcgcgcc tgatgtcctt caactcgttc ttgctaacct
    11041 caaagacgcc tgcgagaata gctatcagca tttcaacctc gactgcatat ctgaacaagc
    11101 cttttgatat ttttgttatg ccttcatcgc ttgcctgagc aatgcactcc gcaagctccg
    11161 gcacaagaac attactcttg gtatagatag ttcttgtcgc aagaaacaat ttcagtgcct
    11221 gctctatcag ttccgagcgg ctgatgtcct gctttgtaca ataatcatca cactctgcaa
    11281 gcacatcacc ggacacataa atccctgttc tgattttatc caaggtcaat cctccttctg
    11341 atatttgtag gcatttctga aatcaataat gccgtttgtc ctgcgcacat cttcagtaca
    11401 cattccgtac agcttaccga catcggtttc atcgacatct gtcaccgccg caagtatgtg
    11461 cgactgtatt gcagactgca ccgccagctt gaacaatgct ctgctgatac gctgctcact
    11521 gccttttacg attccgtcaa tgacagctga taccgtttca ccgagatact ctgtcatatt
    11581 ctccgaatta caatcaagat atcctgcata aaaccttatc gctttttcaa taaaatcgct
    11641 ctgcgacttt gcatttgctg ccgccatatg agtggtcagc agttctttta cttccgggta
    11701 gagccacacc gcttgttttt ctttgttcgc catggaaacg cctccttaat actgttcgac
    11761 tttgttcttt cgctgatatt ttggtcgtga tttcgcagcg ttgccctctt atggtaacac
    11821 acacccaaac cacaaccccc taaaataccg cacaagacct tgcccaaacg ctcgcaattg
    11881 cggtcggctt tgccgtccgc tgtgaatgcg gcgactccgc cgctttaacc tgcaatctta
    11941 ttcctctacg tcataaccgc ttggaattta ccaggtctgt gcaatatgcc aacagccgat
    12001 tctactcaat ttttctcatc ttccttctta aaaaaatatc ttgctgtact tcgctcacaa
    12061 acgccgacaa atcgcttaca acggcgtttt gttggctcgt tggttaaact atccgacctc
    12121 agctgtgggg cggtacaaat cgcccgatag ccgttcttcc atctttagct tattcagcat
    12181 cgccacattc actactgtgt tggagttgtc cctgaccgag accgtgttgc ggcttgtcct
    12241 tgacagcagc aagccctctt tacgaagctg acggagcagc tcatttttgc taatagaaaa
    12301 atgctcaccc tgctcagtgc acagctttcg cacctcggag tgtgttgtgt ctgcgaacag
    12361 gtagtagtgc tcatcgtcct gcaagccgat acagtttttc tgccgcggac tgctgtccgt
    12421 tcccttggct tctacatagc acctaccgct gtccagaaga cttttcagct tttcacaaaa
    12481 cttagttgtc ggattttcaa gctcgatgat ttctgcgttt gcagacacag ctttcaaaac
    12541 gatctcgtcg aacacctttt caaatttatc aagctccgac ttgttgattt gatttttcga
    12601 tttaagaaac acaagcagaa atgcaaaccc gattttcaag tgtgcaagca tgtcgggtgt
    12661 gcggttgtga aacttaattc tgtttgcaca cagcctgtcc agataaaact tgcggtattt
    12721 caaaaacaca tcttcgagtg tttttacaaa agcactttca tcatcaagat acacattttt
    12781 aatccactca atgtagcttt gcataatccc acacagcact ccgtcatttg ctaactgctg
    12841 atattctgac agatcatcaa gctgaacatc gttttcatta agttcaatat tgaagtatct
    12901 tgccgaaccc gacacggaaa tttcaggact gtactccgct gtgataattg cattgcctgt
    12961 aggcggtttt gaagattgta actccgcttt tgaatttagt cgcgcacgac caattctatc
    13021 accgtagtat cgcgaaatat tctgcgcaat gcttttcatc tcctgctcgt gaaacattcc
    13081 gctcggatga aaatcgtcaa tgcaagtcaa cacgtctttt aaatagtata tgttgctcaa
    13141 aatgctattc gctgtgtcgt ggaatgacat cggtaggtcg cttgctgaaa actttccgaa
    13201 gaacgaaagg aacagcgctg caagtgtgga tttacgacta cccgtcttac caatcagcgc
    13261 agtcacaaac tttggctcac agcctgcact tttcagaaag tgattgagcg gacacagaaa
    13321 cgttaccgcc agcagcggta gcataacaca ctgcggtgca aaactgtttt caagcattgc
    13381 agacaagtaa atcaagtcct cctgcgaacc cttttcagtg aaagaatagt ttttcagctt
    13441 gccctgcagt tcaacggtaa acggactgtt ctgcgtcggc atcaggaaca catactcacc
    13501 gtttatcttc ttccaaccag tttggagata catagtttca aatttcacct cacctttcgt
    13561 taacataatt gcgtggcaga ttttacttag cacgttttgc ttaggctgtg cagcacccaa
    13621 tgcaccccag cggtttaaga gccatttcat gttttgcatt tcgtctgcgg aaacaatctg
    13681 ctccgacagt ttaattcccg acttgtgaac cgcaccgacc ttgaacattt tcttttgctc
    13741 tgtgccgtca tctctggtga tttccgaaat aagtgtggga acaaagtctg caagtttcat
    13801 tggaacgacc tgatctttca cctgaaccat ttcataaagg caattgcttt taacaacgta
    13861 gggataattg tagactgttt catcaatcag ttttgctttt tttccttctg tcatgatttc
    13921 tgacattggc atcactcctt ttttaattta tgtagttgtt gtggtgtgtc ctgcgctttc
    13981 aaacattagc cgaacgcccg acagcttatc aggctcatag agcagcgttc ctattgcttg
    14041 cgacggttgg tatcacgctc gagaacgtgc aatgtcagca agctgaaacg taagtatccg
    14101 tttctgattt tcatcgccaa ctgcagttgc tagatgcagg ttgaagattg tatttgtcgc
    14161 tcgttttgaa atacaaaagt catggcgtac atcttcagca gctggaacag ttcatcaccc
    14221 ctccgtcaga aataacgtat tcggttaccg attattcaat tttcaagatt ccgtcaatca
    14281 ttcgctctgt atattaatta ggcgaatttt ttctaattta tcaaatcgca ttacaggcaa
    14341 aaaatccatt cactacatgg tttcaaatgc ttatatcaat tgtacaataa aagttaccac
    14401 aaatctgaaa aagtgtgata taataaatat aggtttttag agctgaccta taaatacact
    14461 tttgaggggt acttgacatg aacaatgcaa tttcaaacaa tccgaattac gatatgcccg
    14521 acgacagttt ttttaatttc tattttctga ttcacaagaa tagaattttc acaacaaaaa
    14581 tagctttgaa ggttggcgaa atcacaacgg ctctgctgaa tatgaaagct gaggacatga
    14641 aaaagattta tgacttaacc gtctcttttg cgcggtttgc agataataat atggcgatga
    14701 aaaacgagca gactatgacg gaatttgttg accgcataat cgaaatcgaa caaattgcgg
    14761 tgactttacc accgtactgc tatgtgaata ttgacctgga aaaagaaaaa cagcgtgccg
    14821 aaataatgaa aagcaaggct ttctacggca ggctgtatga tatgacgtcg gaggagtttg
    14881 cggagtatga gaggtacaaa aagttgtttt cggggtacgg gattgggctt tacataattg
    14941 cctgctgtat agccgaaatg tcagatgaat attttacacg gcttaagaaa cgtgacgaaa
    15001 gcaactatgc gattgcgtgg ggtgcgttta acgaatattc cactctttcg agtgagctga
    15061 tggcgagcgt gccgtactat gaaaaggcaa gagttcgtga aaccatggac gtaaatatcg
    15121 gcgtttcggg aatgatcgat cccgatgata aagacaagac atgggtggtt gatacctgcg
    15181 aatttcataa tgcgcaaagc gttattcagt acgatttttt cagaggaatg cagtatggca
    15241 gagcgccttt tcgctgtcac cactgcggac gttttttcct tgcgactgac agctacaaaa
    15301 cgttttactg caatgaaaag tctcccgaaa atcccaacag aacctgccgt cagattggcg
    15361 cgaaaaacaa gcacaaggaa aaggctgaga acaatccgat tatccagacc taccacaagg
    15421 cgaggaacaa atattatcag cagaaattcc gaggaagtat ttcggatgcg gattacgaca
    15481 aaattctgca ctaccttgaa accatgcgtg acaaggcaat tcggggcaaa agtgacgaga
    15541 atggaaatct gataacgtac aaaacgctcg aacagctttt tgaaaaggac acgctttaca
    15601 agacattaaa tatcggggga gaacaatgac caagaacaca aatgaacagc tgattcagga
    15661 taattaccgc aacgtatacg cacttgcaca ttctcttgca aaatcataca atcttgcatt
    15721 tgaggctttt gaggacttgc agaaggtcgg ctacatttca ctgattgagc agtcgaagac
    15781 ctttgacgag aacagcggtg tgcctttttg gacatatgcc tgcaagggtg ttaagttgaa
    15841 aatgctcgaa gaactccgct ttattctcga tacggtgagt atttcgcctt atttgaacaa
    15901 gcagatagga aaggtgagga aaatcgtttc agccaatgcc gaaagcaaca catccgaact
    15961 tattgaaatc gtaagtcaaa tactcaaaat atcagcggac aaatccgagg agttgctgat
    16021 tttaagcaca aaagagaaaa caagcattga tgagtaccaa tcaatgcttg aaagcggtga
    16081 gggtatgcag gatgacaagc ttgaagatat gcttaagtcc gattgtctga atcccgaaga
    16141 tttaatgctg gagcaggaaa atcaggcttt tgccatgctg ttggcggaca gtctggacga
    16201 cattctaagt catcggcaag cggaatatat ccgaatgtat tttggcatcg ggtgcgctcc
    16261 gcagaccttt gaacaaattg gggaggagtt ctgcgttaac gccggcacgg tggaaaaggg
    16321 cgttaagtcg gctctgaaaa agttgaaaga gtatattgac tgacctatgt tctatcgagt
    16381 ttccagaatg ggaattcagg tagtatgcgt actccatttt tgctccgtat taccgcatta
    16441 caaagagcta tttgcccagc attctcagcg ttttgcgagg tttcggctcg cattacattt
    16501 gcataacaca aaacaaacaa gcatattaca acggagtggc tgtagtacgc ttgtacgcta
    16561 ttgtatgctt tttttgaggc gtggtattac tcataaagtg caggttttac ggcatttgcg
    16621 aggatgtaag ctttgtacgg tggattttga cggagagcta caacttactc agattttcat
    16681 tcttgccgag gagcacgtca atcatcagct tttcgccgtc acggaatccg gctgagtagt
    16741 tatccacgga cattacatcg ttaagctaag actgggtgtc acacatcttt ttgagcagtt
    16801 cacctttttc atctccgaga attttgatta tctggtcata cagatcagcc tcggttttca
    16861 tcagcttggc atactgcgaa ttgtcccgaa tttttgcgtc gaaaagccta aattgccgta
    16921 gtaaaggtct tttatatagt tcatttttga tatatccgtg attggtttgg aagtacggta
    16981 tatcacaatt attcgtggaa tggtagcggc agatgtgatg aaaatcgagt ggcggatttg
    17041 ggaagaagtg ataaattaga atcattttac tgattaactg gcgttttagt cactgttaaa
    17101 ggcaggtgtg tgttacattt gcgttaaaaa atgtaaacat atattgaaaa aatatgaaaa
    17161 gtatgctaca atgaaatgaa tgcaaaaatg actttttata gcttacgctg atgaagggta
    17221 ataaggtggt cgagattgcg gaaatatgag ccgattttct gttgttcctc tattgttgtc
    17281 ggcatcatca aatccatatt tgcaatttgt ttacctgata cttcgacaaa tgtagaacct
    17341 gcaccaatat gataccgtgt ccgatatgcg gtaagagaac ggaatatatc ggggcttatg
    17401 aatgaatata aagggagaga tattatgaag aacagcgtta aaagaacaac agcagctata
    17461 ctggcgacaa tgatgatagg cacaggaatg tatgcaccta tcgccgatca ctataatctt
    17521 ttttctcaaa cgtcaataac cgcagaggcg gcaagtgtcg gtaaggtaac aggtcttaaa
    17581 tctaatactt taagcaacag cgagataaag cttaaatgga gcaaggtcaa gggtgcaagc
    17641 ggttataccg tgtatatgcg taagaacgga aagtataata aggtcgctga tacaaaaagc
    17701 acaacgtaca ccgtaaagaa gcttccaaat gccacgagag agaattttaa ggtaagggca
    17761 tataagaagg taaatggtaa aaaggtatat ggctcttatt cttccaactg gaacaccgcc
    17821 acaaatccac agccttgcaa gggattgaaa gtatcttctg taaattcaga cagcattaag
    17881 ctttcgtgga cgaaaattgg ctgtacaaac tatcgtgttt tccaatacat aagcggtgag
    17941 tggaaggaga taggtaagac cacaggaacg tcctacacca ttaagaaact cacgccacag
    18001 acagagtacc agtttaaaat aagggcttgc aagaaggacg ataagaagaa aagcaataac
    18061 cattatggta aatattcaaa agttgttgct gccacaacga gaagtggcaa aataacacag
    18121 gcagacatag atgctatgaa ggcagaactt acagcgtatt caagagaaaa ggcggagtat
    18181 atca
//
LOCUS       DAWEMR010000033        18094 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1285_length_18094_cov_12.465990,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000033 DAWEMR010000000
VERSION     DAWEMR010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18094)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18094)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18094
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1285_length_18094_cov_12.465990"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2868..2877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3436..3445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9286..9295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5280 a         3726 c         4281 g         4777 t
ORIGIN      
        1 ttacctttca tttatagact gggcatgacc aactttgtac ggacatattg tgtgagttcg
       61 tattaaggtt ggttttttta ttttataggg ggatttttaa tgaaaataat caaggtgata
      121 aataataaca ccgtaagcgt catagacgat aaaggaaaag agcagataat ctcaggcaag
      181 gggataggct tcggaaaaaa atacggcgac gagcctgata aggataagat taagaaaatg
      241 tatcttgtca cagactcaga gcttagaaaa aggcttatcg agtgcctgac cgagatacca
      301 tatgagcata taaagcttac ggcagatctt gtggactata tttcttcaca tatcgacgga
      361 cagctaaacg agtcgctcat aataacactt tcagaccaca tctcatttgc cattgaaaga
      421 aaaaagcagg gacttgaatt tgaaaaccca cttatggacg ccataagcga ctgcttcccg
      481 aaggagcttg ctcttggaag atattgcgtg gcggagatat atcaaaggct tggcgtggag
      541 cttagtggtg atgaagcggg ctttatcgca atgcacatca taaacgcaag gctcggcaca
      601 aaaatggaac agatacagga tatcactaaa atggtgaaca gctgtgcaga gatagcggac
      661 gcgtgtctgg cagggaagat aaataagaac tcggtggcat atgaaagatt tcttgtgcat
      721 ttaaaatatc ttgcgaaaag actcttcaaa tctcaggagc ttcctaatgt tctcagcagg
      781 gacgaggagt tcgctgatat cataaaaaag aaattcaaaa agcagtataa atgcgcaaag
      841 catatgcagg atcatatttt gaagaattat gctaaaacta tatcggaaga tgaaatgata
      901 actcttacta ttcatctgaa aaaaataagc gtataggcat tattgccaca gcttatatat
      961 aatgtattcg ctgcggtatt atcattaacg gtaccataat gataattcga ctgagatata
     1021 attttttgga ggatattatt attatgaagg ataaaatttt cggcgtattg cagcgtgtgg
     1081 gacgctcttt catgctccct atcgcactgc tgccggtggc gggtctgctt cttggaatag
     1141 gcagctcttt caccaacgcc acaactatcg aaacctatca tcttggcaaa ttcatctatg
     1201 agggcggtgt gctttacact atactcgaca taatgagcaa aacgggaagc gccgttttcg
     1261 ataaccttgc gcttctgttt gcaatgggcg ttgctatcgg aatggcaaag aaggaaaaag
     1321 aagtcgcagc tctttcgggt gctatcgcct atatcgtaat gaacaccact atcagcgcac
     1381 ttatcaccgc aaagggcggc gttgaggcaa tggcagaaaa ctccacaacg tcagtgcttg
     1441 gtatcaccac gctgcaaatg ggcgttttcg gcggtatcct tgtaggactt ggcgttgcgg
     1501 cactgcacaa caagttctat aaaacagaac ttccgcaggt gctctcattc ttcggcggaa
     1561 caagatttgt tcctatcgtt tcaactgtca cataccttat cgttggtatc ataatgttct
     1621 atctgtggcc tatagtacag cttggtatct caaagcttgg cgtgcttgtt ctcaactcag
     1681 gctatgcagg cacttggata tatggaatac ttgaacgtgc tcttatcccg tttggacttc
     1741 atcacgtttt ctatatgcct ttctggcaga cagagcttgg cggctcgatg atgatagacg
     1801 gcaacatggt ggcaggcgca cagaacatct tctttgcaga gcttgcttca aagtcaacag
     1861 aagttttctc agtatcggca acaagattta tggcaggaaa attcccgttc atgatatttg
     1921 gtcttcctgc tgcagcattt gcaatgtaca aaactgctcg tcctgaaaag aaaaaggtag
     1981 taggaagctt gcttctgtca gcggctctta cttcaatgat tacaggtatc acagagcctt
     2041 tggagttcac attcctgttt gttgctcctc ttatgtacgc tgttcactgc gtattggcag
     2101 gactttccta tatgcttatg cacattctta acgttggtgt tggaatgacg ttctcaggcg
     2161 gtcttataga catgaccctc ttcggaattt tgcagggcaa cgcaaagacc cactggatat
     2221 ggatagttgt tgtggggctt atctatgcag ttgtttacta ctttgtgttc tactttatga
     2281 taacaaagat gaatcttaaa acacccggca gagaacctga tgacgttgaa cctaagcttt
     2341 atcgcagaag cgacgtgaac gaggctaagg cggcaaaggt aaagagcaca gacaagaggg
     2401 cttcagacgt tgtaagcgca ataatactca aaggtcttgg gggtaaagat aacctttcag
     2461 acgttgactg ctgtgcaaca agacttagag ttacagtaaa tgacgcctca aaggtcatgg
     2521 acgatatgct caaggccagc ggtgcttcag gcgttatcca caagggcaac ggcgttcagg
     2581 tcatttacgg accaaaggtc tcagttataa aatcagacct tgaggatttt atcgattcgc
     2641 cacacagcga tgaccctgac agcattatcg gcactgagaa agcttctgat aaaatagaag
     2701 aaaaaccgca gaccgagagt gcagaaaatg cagacacaaa tggcaagaca ataacgcttt
     2761 actcccacat gaacggcact gccgttaagc ttgaggacgt tgaggacgag gtgttctcac
     2821 aaaagatact gggcgagggc gcagccgttg agccgtcaga gggcaagnnn nnnnnnncat
     2881 gcgacggaaa gatagacagc gtttttgaca caaagcatgc cgtaaatatg gtatcggacg
     2941 atggagtgga gatacttctc catatcggta tcgacactgt aaagcttggc ggacagtatt
     3001 ttgaagccca tgtcagtgac ggtcaggagg tcaagaaagg cgacctgctc ataagttttg
     3061 atatggataa gataaaagcc gcaggctata aggtgacaac gcctttgata ataggcaaca
     3121 cggacgatta tgcgtctgtt gaacctgttg cacaaaacag cgtttcggca ggagatatga
     3181 tcttgaagat aaaataatta tttggaggta ttcatcatga aaacatttag ctacactatc
     3241 acagacgaag ttggtatcca cgcaagaccg gctggacttc ttgcaaagaa agcaaaggag
     3301 ttcgagagcg tttgcactat cgaaaagggc ggaaagagcg tgaacatcac aaagcttatg
     3361 gctcttatgg gtcttggcgt taagcagggc gatactgtta ctgttacctg cgagggtgca
     3421 gacgaggaca aggctnnnnn nnnnngcact caaggcattt tttgaggaaa cactttaatt
     3481 attatctgtc agctgggcgg taaaagccgt ccagccggcg ggacgttttt gcggcgtgtt
     3541 tgcaggagcg tctcatcggc tggaaacagc atgaggatca aaattataaa atctatgaga
     3601 aaaaaagggg cggatattta tgactaagta tacaggaaaa ggcgtttacg gtgcggtagc
     3661 aatgggtaag atctcagtat tcaaaaagca ggataccgct ataaaacgta ttcacacaga
     3721 agacagcgag ggagaaaaga agcgagttgc caaagctaag caggcagcca cagaacagct
     3781 ccagagcatt tatgacaagg cactcaggga ggtgggcgaa acaaatgctc agatctttga
     3841 gatacacatg atgatgcttg aagatgatga ttacaacgag tctatagaga atatcatcga
     3901 cagccagaag gtcaacgcag aatatgcagt ggcagtgacg gcagacaact ttgcggagat
     3961 gtttgcttct atggacgatc cttatatgca ggcaagagcc gcagatgtaa aggatatttc
     4021 caacaggatc atcgcaaacc tcacgggcaa tgtcagcgac ggttcagcag gtgacgacaa
     4081 gatgatagtt tgtgccgatg atcttgcacc aagcgaaact atatctcttg ataaagacaa
     4141 ggtgcttgcc ttcgttactg cacatggctc atcaaattca catacagcta tccttgcaag
     4201 aaatatgaac atacctgcgg taataggtgt aggaagcaaa ttcctgtcag aaataaagga
     4261 cggcgacttt gctatcgttg acggttttac aggggagata ttcgttgacc cagatgaaaa
     4321 gaccaccgct gagcttaccg caaagcagaa agcagacgag gagaaaaagc gccttttgca
     4381 gaccctcaaa ggcaaggaga atgtcacaaa ggacggtaaa aagataaaca tttatgcaaa
     4441 tatcggaagc gttgacaaca tcggtgctgt gcttctcaat gacgcaggag gaataggtct
     4501 tttcagaagc gagtttctct atcttgaaaa ctcagatttt cctacagagg aacagcagtt
     4561 tcatgcttat aagcgcgttc ttgagagcat ggcagggaaa aaggtcatca taagaacgct
     4621 cgatatcggt gctgacaagc aggtggatta tttcggcctg aaaaaagagg aaaacccagc
     4681 tttgggatac agagcaataa gaatttgtct tacaagacct gagatattca aaactcagct
     4741 cagagcgctt ttcagagctt ccgtatacgg caatctgggc ataatgttcc ctatgataac
     4801 aagcgtttca gaggtagaaa aagcccttgc tatgtgtggc gaagtcaagg ctgagcttaa
     4861 agaacagggt ataacagttt ctgacagtgt tgagcttggc atcatgatag agacccctgc
     4921 ggcggctatc ataagcgata agcttgcaaa gcttgtggac tttttcagcg ttggcactaa
     4981 tgacctcact cagtataccc ttgcttgcga cagacagaac cctgatatag agcagttctg
     5041 cgacactcac catgaggcta tacttagact tattgaaatg tctgcagaga atgcccacaa
     5101 gaacggcgca tggataggta tatgcggtga gcttgcggct gacactactc tcacagaaac
     5161 cttcctgaga atgggcattg atgagctttc agtttctccg acctttgttc ttaaggtaag
     5221 ggacgctgta agaaatattg acctctccaa ataatttctc atagatcttc acacgaagaa
     5281 cacaccctcg ttcataggga cgggggtgtg ttcttttatt tgacagatat gctttaatgt
     5341 gctaaaatat cttatcggca caatcgtgtt tgcacataag tttgttatca atgcacaaaa
     5401 aataaaagta aaatttgtat actattttgc cataaaatct cccgtgcaga atcgtatata
     5461 agttatcgag gggtgtgaaa gacttgaccg acactgagtt cgatttcagg gtggagctga
     5521 tcctgaaaaa tgacaaaaac gggcttaggg agatctatca atgctacggc aaggtgatat
     5581 ataacatcat gcttgacatt gtgaaaagtc ctcaggacgc tgaggacctc acatcagatt
     5641 ttttcctaaa gctatgggac accgcaggtc agtaccgaaa gggaacgggg cataagcgat
     5701 atctgactgt aatggcgaaa aatctcgctc ttgactttat caaaaaaagg cggcacgaaa
     5761 cgccgagcct tgatgatgaa gaagcagggc ataaagaaca gcaggacagt atgcgggtag
     5821 acgacgatgt gatcggaaag ctcaccttcg aggaggcttt gaagctgctt tcagaggacg
     5881 aaagggatat cctgaacatg aggcttggaa tggatatgac atttcgtgag ataagtcggg
     5941 cagtggggaa gccgctgggt actgtcacat ggaaataccg tcaggctgtt ttcaaactaa
     6001 aaaagaccat aaaggaggac agtatctatg gctaagaaca taaaatttct ttacaaaaag
     6061 catatttctg atactgcccc tgacatggaa aagctctggg acaggataga gggacagctt
     6121 gaaaacaaaa cggacaaaga tcggacacag ataaaagtaa gccacagaaa ttacagaaaa
     6181 tttgcagcgg cagcagcctc gctcgtgctt attatcggcg gtgtaagcct ttacgccgat
     6241 atccgctcag agtcggacag tgtttcaaag catagcgtag aaacggctga caaggctagg
     6301 cttgaaaaca gagaagaaaa gacatacgaa cagctcagtt tcagccacac agacacaaag
     6361 gcttatgagg acagctacat tccaaagggc gatgattatt ttacagagga cagcgttctg
     6421 gaagatacag acttctttgc agatgtgaca gtgctttcag caaggcttga cagcgacagc
     6481 gcacattaca ccctgagaat aaacagcctt gtatcaaaga caggagaaga aagcaaaaca
     6541 aatattacca ttgaaagctc taccccttat atcatgcagg aaaacagaga atatttcata
     6601 ccactgagga aagaaaacgg cacatattct atggtctttg aaaatgcacc tcaaatagaa
     6661 atttctcttg acggggatat cgtttttcaa aacggctgga aaagtcttga cgatgattcg
     6721 ggtattctta aggactcgca gaaaagcaag aacgattatt tctatgaccg aatgagatat
     6781 tcctcgaaaa acgatctgca agacctgctt gctaagtgga ggactatgta gctaacaacg
     6841 caagggcata tcgcccacgc tattttacgg gagatgacag gtatgaaaaa gacaacttca
     6901 aacagcataa aaagaaactt gatcacagct ctgtcgctta tgatgacggc gacaatgctt
     6961 gcttcatgcg gaagcggtta tgacggctcg gagagcagaa agagcggcag ctataacaat
     7021 gaaagaacaa ctgcggaaaa ctacagatac agcaatgtta cagccggaga tactgacggc
     7081 acggcgaata taggaggggg ttacaatacc gaggaatata accatctgac cgaaaacggc
     7141 tataagctcg tttcagacga accgctttcc accttttcca cagacgttga cactgcttcc
     7201 tatacaaacg taagaagaat gataaacgac ggctgggagg tcgaccctga cgctgtgaga
     7261 acagaggagt tcataaacta tttcaagtat gactatgaaa agcctgcgga gggcgcggtg
     7321 tctataaata ccgagctttc tgattgtcct tggaacagtg aagcaaagct tatgctggtg
     7381 gggcttcagg caagggaagc aaaggtcaag gacgttccta caaaccttgt tttcctcata
     7441 gacgtgagcg gctcaatgga ggacgaggat aagcttcctc ttgttcagca ggctttctca
     7501 atgcttgctg aaaaccttgg ggctgatgac agagtttcta tcgtcactta tgcaggcagc
     7561 gacagcgttg ttcttgaggg cgaaagtgga aacaacagag aggagatagt ttcagcactt
     7621 aacagcctgt cagcaggcgg ctctacagca ggtgcggcag gaattaccac agcttacaag
     7681 cttgcagagg agtatttcat agagggcggc aacaacagag ttatccttgc aactgacggc
     7741 gatctgaacg ttggtctgtc aagtgaagaa gagcttaccg accttatatc acaaaagcgt
     7801 gactcaggtg tatatctctc agtgcttggc ttcggctcag gaaactataa gaacaacagg
     7861 cttgaagccc ttgctgataa cggcaacgga aactatgctt atatcgacag cgttgacgaa
     7921 gcagaaaggg tgcttgttac agaaatgagc ggaacgcttt tcactgttgc aaaggacgca
     7981 aaggtgcaga tagagttcaa tcctgaaaat gtttcagcat acaggctggt gggttatgaa
     8041 aacaggcttc ttaatgacga ggactttgag gacgatacaa aggacgcagg cgatatcggc
     8101 tcaggtcagc aggtcacggt tttgtatgag atagtgccaa ataacggcaa tgaaaagtcg
     8161 ctgaaatatc aggataacga gagcaaggtg gctgaaacta gcgaactcag cggcgaaatg
     8221 ctcacagttt cagtgagata taaagaccct gaggcgagcg aaagcaagct tatagacaag
     8281 tctgttatgt gttctgacta tacagaggct gtgtcacaga attttgctaa ggcttgctct
     8341 gcggcagagt ttgcaatggt gcttagaaac agcgactatg caagcggtct taccgctcag
     8401 ggcgttgtgg attatatgga acaaaacaat gttgacaaca gcgagctttt tgaactcata
     8461 tacaaatata tgcaaaacgg cacaggcagc gaggcaagct actggtaaag aactaaatca
     8521 aaggggtgct gcatggtcag cacccctttg ggccataaaa tttacctctt gcatagaaat
     8581 ctgcacctgt cagccaaaag gggtttacat gaaaagaaaa atagattata ataaggctgt
     8641 cagataaaac gggggtgaaa gcgtgaaggt caggatagag atagacgaga atatgtctga
     8701 agacgagata gtcataaggt gcgcccagcc cgatgaacgt gtgcttaaaa tgaaaagggc
     8761 ggcggagtcg gcagagggcg atcctgagat agcttttttt aaagacgcag ttcagttcta
     8821 tcctcccctt gattccatac tgttttttga aacaggggaa cataatctta acgctcatac
     8881 ctctgacgac gtttttcagg tcaaaatgaa gcttttcgag cttgaagaga tacttccacg
     8941 aaagttcgtg cgggtgtcaa aatcagcaat cgtgaatgta aagcacatct attccgttga
     9001 gcgaaacata acctctgcaa gtcttataag ctttgcaggc actcataaac agctttacgc
     9061 ctcacgaagc tattacaagc ttttgaagct cagacttgga gaaaggagat ttctatgaaa
     9121 aacaaaaatc ttatatgggg cttgttcttt atccttgcag gcgttgcgct tattctcgac
     9181 cgtaccggtc tgctgggaag tgtaagcgtg tggagcgttg tgatctcact gcttctcgtt
     9241 cctgttatcg taacgagcgt cagacacctt aacttctggg gaatannnnn nnnnntattc
     9301 ttccctatgg ctatcatggc gatactcttt gacgaaactc tcggtatcga gaagttcacc
     9361 ccatggcctg ttttgggtgc ggcactgctt ctcagcgtgg gattttcatt tattttccct
     9421 tatcgctaca ggtttaccca aagcaagtgg accacccaca ctaccgataa tcctgcatgg
     9481 gataaatcct cagacaacgg ggaatatgtg cgtatcgacg caaaattttc aggctgcaca
     9541 aagtatataa cctcaaaggt gttaaaaaga gttgacataa gctgccgtta cagcggtatg
     9601 gaggtgtact ttgattctgc cgatatggca ggaaacgaag tcgagctgaa tgttgatgtg
     9661 ctttgcggcg gagtggagct ttatatcccg aagaactggg gcataaaggt ggaagccgac
     9721 tgcgttcttg gcggaataga ccagcctaac ggagcttatg atgaaaaaga aaagatcctt
     9781 atcataaagg gcagagcaaa gatcgcagga atagatataa catatgttta gtcttatgtg
     9841 aaagaacaaa agtaaaagct gcggagggaa aactccgcag ctttttgtat gtctgattat
     9901 tcttgaagta tcatcttgtc tgaaagttcg tcctcggcgg tgtcggaata aagcttcata
     9961 agcttttcca ccgtcatgtt tgctctctgc tcgccctcga tagtaagaag cagtttgcct
    10021 tggctcatta cgatagtttt gttgccatat tcaatggcat ttgaaatgtt gtgtgttatc
    10081 ataagagtag tcagcgcgtt ttcctttact atcttgtcgg ttatcctcat gaccttttct
    10141 gcggtcttag ggtcaagtgc cgcagtgtgt tcgtcaagaa gcagaagttt tggtgtaacg
    10201 atagtcgcca taagcaatgt gagcgcctgt cgctgtccgc ctgagagcaa gcccacaggc
    10261 attttcattc tgtcctcaag acctaagtca agctccttga gcctttctct gaacactgaa
    10321 acgtcctttt tcctgatacc gatagtaagc gaacgacgct ttccacgact gtaggcaagg
    10381 ccgagatttt cctctatggt catgtttggc gcagttcctc tcagcgggtc ttggaaaagc
    10441 ctgcctatag ttcttgcacg ttgatgctcc tttaggtgag tgatgttctt tccgtcaagg
    10501 aatatcttgc cggtgtcagc ctcgtagctg ccactgataa cgttcataag tgtggatttt
    10561 cctgcaccgt ttgaacctat tacagtaacg aaatctccgt cagccacatc aaggtcaatg
    10621 tgctggagtg ccttacgctc gttggcagtg ttcttgttga aggttatgtt tatattttca
    10681 agcttaagca ttttaaacct cctatttgtc actgcataca cgcttgtatg cctttgtgat
    10741 atacttcttg atagcaggca gagagatagc aagggcaact atgcaggctg agaaaagctt
    10801 tgtgtagttg ctgtccatac cgagattcat tgcaaaagca agaacaaatc tgtatgccac
    10861 cgcaccgaat atcgccgcaa gtattccgca gaggatcgag tgcttgctga agaatatctc
    10921 accaataatg atagaagcaa ggcctaccac cagcatacct gtaccgctgt ttgagtcgcc
    10981 tgccgaggaa tactgagcgt acattgcgcc tgaaagggat accaaagcgt ttgcaatggc
    11041 aaagcccatt atcttcattc tgtctgagtt tattgaagaa gcccttacca tgtattcgtt
    11101 gtcgcctgtg gctctaagtg acattcctgc ctgagtttta agaaaccaaa aaagcagaac
    11161 agccgcaata ataagcacga tacctgccgc cgcaagcttg taatagtcgc cgaaatattt
    11221 ctgagccatt gtaaatatag tatctttctt atagagtgaa atattaggtc tgtccatgat
    11281 tttcaggttt atggagtaaa gagccgtcat tgtgattatt cccgcaagta taggctgaac
    11341 gttgagcttt gtgataagta ttcccgtcac gcttcctgcg atagctcctg caactattga
    11401 cagcagtatt cccttgatag ggtcgccgtt gaaagcctgc ataactgata ctgcaacgcc
    11461 aagggtgaaa gttccgtcta ccgacaggtc agggatatca agtatcctga atgagatgaa
    11521 tattccaagc gcaaggatag aaaaaacaag acccttttca aatgttcccg taacaacgct
    11581 ttccgtgagc tgagtgttta ttatcacaag aagtaagatc aaaagcacag ctttcggtat
    11641 aagcgagata actgttttct tattcagttt catataattt tcctttcaat aaaaaatagc
    11701 agggagcgaa agtcccgctc cctactatta tactatgttt gatttgattt tgcaatagtt
    11761 taatcaaagt aaactgtctt ttctccattt gcaatatcgt caggaatagt gataccgatc
    11821 gtgtcagcag ttgtcttgtt gatataagtg ctgaggtctt cgttgaatac ctttattggt
    11881 atctcggaag ccttcttttc gccgctgagt atctgagctg ccatgtttgc agtttccttt
    11941 ccaaggtcgg aatactcaat accaacagtg ccaagaccgc cgtccattac cattgagtca
    12001 gcgccaacga acattggaac tttagccttg ttcataactt ctgtaactgt acccatagcg
    12061 cctgcgatag tgttgtcgtt aggtgagaat acagcgtcgc actttgcggc aaggttctga
    12121 gcagcttcct gcacctcaga tgtgtttgaa gcagcgcctt cgataacagc gatgcccttt
    12181 tcctcacagt aagcctttac ttctttaagg gtagaaactg agttgtcctc gcctgtgttg
    12241 taaagataac cgagagtttt tatttcaggc ttcatttcaa gcgcaaagtc aagtatcttt
    12301 gtgatagcga gcttgtcaga tgtgcctgtg atgtttgccc ctgtgctttc aagcgagtcg
    12361 caaaggcctg caccgatagg gtctgttact gcggagaata ctacaggtat ctcctcagag
    12421 tatggagctg cgagctgtgc gcatggtgtt gtgatagcga tgatgatatc gtcaccgtca
    12481 gccttgaact ggtcgagtat ctgctgaaca gttgttgact cgttgttagc ctgctgagcg
    12541 tcgatagtgc agtttttgcc gtcaacatag ccaagctctg caagtctgtc gataacagca
    12601 tctctgatag tgttgagaga tgtatgctcc attatctgaa caatgcctat cttatagtgc
    12661 ttttctgatg agccgcctgc ctttgaatcg tttgaggagt cctccttaac gccgcatgac
    12721 gcaagagcag ccacagccat taccgctgca gcggcaagtg ccagaacttt tcttaacttc
    12781 atatatgtac cattcctttc gtatttacgg ggattttacc ctactactct attaaagcac
    12841 agccgtaaaa gtttgtcaag taaaagttaa aaggattgta gaaatagtaa aagccacaga
    12901 gaaaatcccc atggattttg tatgttttgc agttggttga cagaaatgtg aaaaaaggct
    12961 ataatgatat ctgtaatatt gcagagcagg aggttttgta ttgggactaa aaagggtttc
    13021 cgtgataggg ttttgtcttg cggcggtggc agcggtggta atgctcgctg cagttataat
    13081 aagaccaccg aaaatatata taagcgagat atgcccctca aacagcgaaa catctaagaa
    13141 aacagctatg caggacaaaa acggagaacc aagcgattgg atagagatat ataatcctac
    13201 aaataaagat atttcactta cgggattttc actttcaaaa aatggcggtg gagatcagcc
    13261 tttgggcgga tatgtcatta aagcccatga ctatataata gtatactgct catcagcagg
    13321 ctttgagaat gctgattttc ctcacgctga tttcagcata ggcaaggtca gcgaagcgga
    13381 gataatattg aaatatgact cgttccagtg tgagagcata aaattgccta agcttaacaa
    13441 gggcgtgagc tattctaaaa atgtcaaggg tgaaatgtac gtttcagagc ctacacctct
    13501 tgcggcgaat gcagagaaaa ctatcggaga tacacctgtg ttttctcagg cggcaggcag
    13561 ctatgaaaag gcttttgacc ttgagataac cgcaggagag agtcagacgg tgtattatac
    13621 caccgacggc accgaccctg ccacgtcgga cacaagaaag gtctatgaaa atgccctgag
    13681 aattgacgac aggtcagatg atgaaaatgt cctttcggct tatgacccta tgaaaataca
    13741 gcttgattat cgtgacagca taaaactgcc tgacaaaagc gcagttgata aaggcactgt
    13801 cattagagcc tgcgcagagg gtacaagcgg aaagtgcgga aaaactgtta ctgccaccta
    13861 ttttgtggac gtttcaagtg cagatcataa cgatctgcct atagtatcca ttaccaccga
    13921 tcctgacggg cttttcaacg aaaaaactgg catttacagc ctcggagaag tctatgagga
    13981 atatgacgaa gaaaaccccg accacccttt gaacggatct atccctgcaa actataatca
    14041 gcgtggcagg gagtgggaaa aagaatgtta tgtggagtat ttcgacagcg agggcaacag
    14101 ccttatttct caggattgcg gcataaggat acaaggcggc tggtctaggg cggattatca
    14161 aaagtcgttc aggctttatg ctcgaaacga ttacggcaaa agctcttttg atacagtttt
    14221 ctgggatagc tttacagatg taaacggcga ggctatcacc tcatgcaaga cctttgtgct
    14281 tagaaacggc ggaaatgacg ccaattattc aaaattcaag gacatgatga tacaaaatat
    14341 ggtgtcaggc agaggtgttg aaactcagca gggaacagcc tgcgtgctgt ttatagacgg
    14401 tgaatactgg ggactttata ctctccaatc cgattacagc gacagatatt ttgcggatag
    14461 atacaatgtc gcaaaaagca acgttgtcat gtataaaaat gatgagctga gcgagggtga
    14521 ggctggggac gaaaagcttt tcaatgatat gtataagttt atcacagaaa acgatatgtc
    14581 cattgaggag aattatcgca aagcctgcgc aatgatagat atggataacc ttgtggagta
    14641 tgccgccaca gaaatgtata tcttcaatga cgattggccg cagaacaact atgcttgctg
    14701 gcgcacaaga accatagagc agggcaatag ctatgcagat ggcagatggc gctttgtgct
    14761 tttcgacacg gaatcgtcat gctcgcatta taacgagaag gatctggaaa caaatatgtt
    14821 ctcgtatctt agaagccaaa gctatacaaa gtttggcgga atactttgca gccttataga
    14881 taacgaggaa ttcgatttga agctcacctc ggcaatgtgt cagcttggaa gcgtgaactt
    14941 tactgcggag cggttcgggg agtatctcga atattataag aatatctatt acggcgagct
    15001 tgataattat ttcgaccgtt ttccaacatg ggcaaacctt gcgaaagcta cagaccctat
    15061 gataatacgt tggcagaatt ttattgaggg aagatatgat aaagtgcttg gctatcttga
    15121 aagagagttt gactattatg agcgcagaac tgtgaagata tcagcggata acgagcaggg
    15181 aagcgttttg atagggggcg ttgaaataga aagtgattat agcggcacat attttgacgg
    15241 ctgtgagata aaattgaacg cacaagcaaa atcaggctgg cattttgacc attgggaggg
    15301 cgtaaagggc gataataccc aatcggagat aaaggctaag gtgaatggtg atatgaatat
    15361 caaagccgtt tttgaaaaag atattgttta gcataaaaaa ggtctgtcac caaaatcaag
    15421 tgacagacct tttactatat aacttaacct gcaaaatcaa tatctacaga gcatatctca
    15481 gcgtcagttc ctaccctcat gaaagtgccg tatacaccaa caccagaggt gacaatggag
    15541 tacatgttat ctttctttat caggccacat gggttttccc agaaaagacc tattgtcaga
    15601 ttgcctggga aaagctgccc gtcatgtgtg tgacctgaga aatctatgtc tgcacctgcg
    15661 tcagcggtct tttgaagctc gtcaggctcg tgggaaagca cgaatatcgg cttggtcttg
    15721 tcaagatcct tcgtaagctc tgatatctct gcacgagtgc cgtcctcagt accgggcttg
    15781 tcagtgtcac gcctgcccac aagataaaat tcatcgttta tcaaaacaga ctcatcgttg
    15841 ataagcttta tgttacactc tttcataaag tttgtcattt tctcgttgtg gagctgtttt
    15901 ccgccccagt caaaggtaaa tcccatgaga attttttcat ctatatcgtg gttgccgtag
    15961 acagcataaa cgccgtattt gctctttatg gacctgagct gtgcctttat cccttcagga
    16021 tcgtccatgc cgtcatagct gttgtcaaaa atatctcctg ctattataac aatgtcagcg
    16081 tcctgctggt ttattttctc aaccatgttg gtgatgtgat ccacaccgat agcgtaaccc
    16141 atgtgcaggt cagccacaag cacagctttt aaatgcttgt cactgccgca tgatttgttg
    16201 acagttacgc tgtattcatt caccttgatg tttcgtgcat tgaaaatgcc gtaaagacag
    16261 gtcgccaccg cacaagccac aaccaccgaa cctatggaaa taacgcttcc acgggaaaaa
    16321 agcagcgtgt ttttcagctt cgtgtgcttt gcgataagtc ttagcaggtc aaaaagcacc
    16381 acatacaaga gcgagtagag cataatgcct atccagtatg tagaaattcg ccttatgacg
    16441 atagcaacgg cggattttgg aagcagaaaa gctataacag gcgaaagcgc catgaaaaga
    16501 tagaccactg caaaaggcac tttgaaacgc agccagttaa atctgtggtt gcagtgcttc
    16561 atccagtaga aaaatcggaa catcatgtat gatgatacgc ccagataaaa gggcagaaga
    16621 aatagaaata tcattattat gccaccttat agataagtga ggaatgagga gtgaggaatg
    16681 aggaattaag gtatcgcctg tggcgatgat tttttgtagg ggcgaacttt ggtcgccctt
    16741 gccgtatgtt tataatacaa tgtaaaagtc ccacggattt tgccgtctgt ttgtgctgta
    16801 tttttattcc tctttaaatc tctccaaaaa actgtgtctg ccctcgtcat ctttgtatgc
    16861 aatgacctga ttgagcataa cgttttccac gctcttgaaa atgttgtatt ccacctgcgg
    16921 attaacgagc tttagctttg cgataagctg ttttatctcc atacgctttg atactgtcct
    16981 gctgtcaggt gcgcaggtgg tgcaggtgtc ggtgaatttg caggcacact gtatgaaccg
    17041 cagcccgtta tagtcacgcc acctttttat gtcgtcctct ctgaccatat agagaggtct
    17101 tataagctgc ataccctcaa aattcgtgct gttgagtttt ggcatcatgg tctgtatctg
    17161 tccgccgtaa agcattgaca ttagtatggt ttcgataaca tcatcatagt gatgcccaag
    17221 tgcgatcttg ttgcagccaa gttcctgtgc tttgctgtat aaatgacctc ttctcattcg
    17281 tgcgcacaga tagcatggat ttttctccac attgtataca gagtcgaaaa tatcagactc
    17341 aaacactgtt atgggtattc caagccgctt tgcgttgcgc tcgattatct cacggtttgc
    17401 agggctgtag cccggatcca taacaagata ttttacctca aaagggaact tgctgtggcg
    17461 gtgaagcatt tgtatgagct tcgccatgag cattgaatcc ttgccgcccg atatgcacac
    17521 cgctatcctg tcattaggct ttataagctc atattcgttc acagattgtg tgaatctgtt
    17581 ccatatctcc ttgcggaatt tcttttgtat gctccgctca gcgtcctgcg agatatcctc
    17641 tacgacctct tttgaaagct tttctctcag ccagccataa tagccaccct ctaggttttc
    17701 agcgtcatag ccaagctctc gaagcttttc ggcctcagtg tcgcttataa gtccgctttt
    17761 acagcagata agaagctttt tgtcttttgg caggtcgctt ttatcaagct tttcggcagg
    17821 gatgttcact gctccgtcaa tgtgaccata ctcaaaagaa agctcgtccc ttatatctat
    17881 tatcagcgtg tcgccgcttg catttatcat ttgtcagttg acctccgcaa aatttatcac
    17941 tattctttat gatagcataa aaatcaactt ttttcaagct ttacgcaaaa ataaaaggcg
    18001 ttgtaaaaaa acagcgctca aagagtcaag atttcctgct tttgggaaat ctcgactctt
    18061 tttttatgcc agatcgcaaa aataactatt ttgg
//
LOCUS       DAWEMR010000034        17292 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1378_length_17292_cov_11.672275,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000034 DAWEMR010000000
VERSION     DAWEMR010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17292)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17292)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17292
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1378_length_17292_cov_11.672275"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1098..1107
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2149..2176
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5153..5162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10692..10701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5028 a         3714 c         4005 g         4487 t
ORIGIN      
        1 tgtaaaaatt tttaggagtg ttatttatga ataagaatct taatcttcag gatgttatac
       61 tcaaccagat cagaaaagac aagatagcgt caacttttat ccttatgaac ggctaccagt
      121 tcaaaggcgt tgtaaagggc tttgacagct atgttgtagt tctcgattgc gagggcaagc
      181 agaacatagt ttataagcat gctatctcaa ctatcgttcc gtcaagatat atcaacgttc
      241 ttgacgccgc aggcaattca gatatttaag agggcagtaa aaaatgaatt ctctgaccgt
      301 taaaatactt accggtctgc tttctgtgct gataatcaca actatcggca gtcagatata
      361 cagtgcactt acagagcgtc atgataccga ggaagctgtg cttgttacta tcaatgagga
      421 tattgctttt aagggcgtta taatcagaga tgaaaaggtg gtcacctatg acggcggcgg
      481 agttttggat tatacctatt ccgacggcag taaaatatct gttggcaata ccatagcgtc
      541 agtttattct tctgaggacg ctgttacagc gaaaaacaag ataaagatac ttgaaagcga
      601 gatagccgcc cttgaaagag cacaaaatcc gggtacaacg aattatgttc agcctgaaac
      661 gctcaaaagc aagatagata aggaatataa ggagcttctt tcatattctt ctcaggataa
      721 tttctctgag tttgccaaaa caagagagga tatgtccctt gtgatgaata tttataatat
      781 cgtcacaaag accgagcaga gctatgacga aaggataagc cagcttcagg cgaaagtcaa
      841 ggagttggag gaacagacct caggctcagg aaaaacgatc actgctgacg aaacaggcta
      901 ttttgtttcc tatgcagacg gctatgagag cgagcttact atggagaacg cttcccagct
      961 cactgacaag gatatccaaa aggtcatcga ccagcctagt aaggacgcac cgaacgccat
     1021 tggcaaaatg ttttccgact attcatgcag gatagcaggg atcatggaaa acgataaaag
     1081 gataacagag ggcgcatnnn nnnnnnnctc agaatgacgc tcagtaccac aaaaaacatc
     1141 tatgacgttc atgtggagtc tgttaagcct tgtgaggacg atgagaacaa agtggtcgtc
     1201 gtgctttcct gtgacagact tgacgaagct cttgttgagt caagagtgca gtctgcggag
     1261 cttatcttcg acgagtatca gggtcttaaa gttccaagaa gtgcgatacg ttttcagggc
     1321 gatcagaagg gcgtttatgt gatactcggc aaggacgtta cctttaagaa gatcaatgtt
     1381 atttatgagg gcgatgatta tgtgctctct gaaaatacct caaacgagga ttatctgctg
     1441 ctttacgatc aaattttgtt agaggtggta tcagatgaag atgtgcagca aagtagatca
     1501 gacagcaacg ctgtcacctc aggataatta ttttccggag gttcttgaaa actgtaagga
     1561 aatatgttat aatgtagaga acgccaaagc aaaatggcgt aagcctgacg aagaagtcag
     1621 gataatggct gtcacaaaga cagtgccgcc tgaaaaggtg aatttcgctg tggctcaggg
     1681 ctttaagctg ctgggcgaaa acagggtgca ggagtttctt tccaagaagg aagcttacga
     1741 taaaagtgca gaagtgcagt ttatcggaca tttgcagacc aataaggtga aatatataat
     1801 caatgatgtt actctgatac agtctgtcga taacctgaag cttgctcatg agataagcag
     1861 gcttgcagtg aagaataata aaactatgaa cgtccttgtg gaaataaata taggcgacga
     1921 ggctactaaa agcggcgttt ccgctgaaat ggctgaggag cttgtaagac agacagcgca
     1981 gctgcctaac gtttctgtta agggacttat ggctatccct cctgtaggct caggggagga
     2041 cgtttttgaa aaaatgcacg gtatctttct tagtgtaaag gaaaaggata taccgaacgt
     2101 gtctatggat attctctcaa tgggaatgtc tggggactat gagcttgcnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnngatt aaaaatgggt gcaatgcaga aattaaaaaa tctctttgta
     2221 ggcgaagatg aacttgagca ggaggacgca atgtatcagc agccggtgta tgagaataaa
     2281 acagaataca acgaagcacc aaagaacaca ggtgcatatg gcaataacgc acgtccggta
     2341 agaatggagc aaaccacgac tttgcagata gttcttgcaa gaccaaacga tttttcagag
     2401 gtcaagtcta tcggcggcga tataaatgag ggaaagacag ttcttctcaa cctggaaact
     2461 gtcaagagcg aggacgcaaa gagaatactt gactttatct caggcgttgc gtatgcaaat
     2521 ggtgcagaca tcaagatgat ggctcagaaa actttcgcta tcatgcctag aaacgttggc
     2581 ttctcaggag ttgaccttat gagcgagctt gaaaataacg gctacagctt ctaaaaatgg
     2641 ctcgtgctaa atatcagttt ttgcccgacc tgtctgagga cgatatccgt ctgttagaca
     2701 gagtgtccga ttggataatg gcggctgatg aaaagtatat aacaaagttt tctgcgtttc
     2761 tcgatgaaag gcagtgtctg ctgtgcgaaa aactcatggc gtctgtaaaa tatgagaatt
     2821 ataggctttg gggcggatat gaaaacgccg caaggcgtat gctgtgcgtc tatcctgaat
     2881 atggcgaggc agaaagtgag gattttccta tcaccgcaat tacactgctg tatcgcaaag
     2941 aggataaaat aactcacaga gatatcctgg gctcgcttat gggactaaga ataaaaagag
     3001 acagcgttgg cgacattctt gtgggagagg gaaagtcagc ggtgttcctg cgttcttctg
     3061 tggcaggcga ggttacaagc gtcctgacaa aggtcggcag agcaggagtg aagctccggg
     3121 agggctatga cggcagtata catattgagc agaaaactca ggaaataagc ggtactgtgg
     3181 cttccatgcg tgcagattgt atcttaagtc ttgccgcaag gatatcacga gaaaagtcag
     3241 cacagcttat aaaaaacgtg ggcatcgaaa tagaccatat gccgaaaaat tcaccgaaaa
     3301 tacttcttga agaaggctgt acattttcag tgagaggata cggaaaattc aagctgcttt
     3361 cagttgacgg cacaactaaa aaagacagaa tacatataac tttaaataaa tttgtgtaaa
     3421 gatcacggag gtcctagtat gctaagataa tcaagcttgt tgagaagata aacgagtaca
     3481 gagaggacga ggaagctatc cagcaggctc ttgtcgtggc tcagaaagag tcaaataaga
     3541 tacttactga ggccaaaact caggctcacg atatgatcga gtctgccaaa acagagcagg
     3601 taagactcgc agagcagagt gcctctgagt gtgagagaat agttaaggag cataaggaaa
     3661 agtgcgctga gcttatcaga actaataccg agataacaga aaagaagata aatgaagtaa
     3721 gagccgctta tgacgaggag cttgctaagt ttgagaacct caaggctgag gtaactcagt
     3781 tcaaggctga ccttactcag ctttataata agcagataag acttattatg gagattcctg
     3841 cgtccgagcc tgccgaggat acagcagagg agtatgagga agaagaaaca gtcgaggaaa
     3901 ctcaggaagc tgcagaggag caggcagagg aaacagccga gccggagaaa actgttgagg
     3961 ctgaatctgc acctgttact gacagtgagc acgttgagaa gatacttaac actggttctt
     4021 tcgaccctgt tataccaaag acagctcctg ctgagcttaa atttggaaaa aataattaat
     4081 atcaaggctc tgttttccgc agagccttac tgttcataaa tggagtaggg aatagatgtt
     4141 tgtaatttcg cttgttatca tggcagttct cgttgccgcc gaccagctca taaagcttgc
     4201 cgtgacaaat aacctgccgt tgaatggtca gcctgtcgag gtcataaagt tcggcagctt
     4261 taaggtgttc agcctgacac atataagaaa ctcaggagca gcatggagca tgatgtcagg
     4321 aaagacctgg tttcttatcg gtctgcctgt tgtcgtattg atcgtggcgg tgatctatat
     4381 gtataagata cgcaaaggct caaagctgga gcttgtgtcg ttggctatgg tgcttgcagg
     4441 cggtatcggc aaccttatcg acagaataag ggtaaaagaa gttgtggact atatcaagtt
     4501 cgagcctatc aatttcccta tcttcaactt tgcggatatc tgtatcgtta tcggcggaat
     4561 tttgttcgtt atctgcttct gctttcttga cgaaaagaaa aagccgatcc gtgacgatat
     4621 aaaggtcaaa gacgaaaatg acaaagcttg attttattgc cactgccgag caaacaggcg
     4681 agcgtatcga cagatttttg tcagcgcagg atacaggtct tacacgttcc gctgtgcaaa
     4741 agcttatcga ggacggtggc gtcacagttg gaggcaaaaa agtatcaaaa aattataagc
     4801 tgcataatgg tgacagcgtt gggataatta ttcccgaccc tgtggagctt gacgtcaagc
     4861 cacaggatat ccccgtggaa atagtctatg aggacagcga gctgcttgtg gtgaataaac
     4921 ctaaaggcat ggtggtgcac cctgccgcag gcaatcccga tggcactttg gtgaacgcat
     4981 tgctttatca ttgtcagggc aggctttctt caataaacgg cgtcattcgc cctgggatag
     5041 tccataggat agacaaaaat acaagcggtc tgctgatcgt tgccaagacc gacagcgcac
     5101 ataactttct tgcggctcag ataaaagagc atagctttac aagagagtat gannnnnnnn
     5161 nntgtactca caggcggtcg cctaaagacc ccgaccggca cagttgacgc acctatcggc
     5221 agaagcaaat atgacagaaa gaaaatgtgc gtgacagagc aaaactccaa gcacgcagtt
     5281 actcattatg aggtgctgga ggagttaggt cagtattcgc tggtgaaatt tcgccttgaa
     5341 acaggtcgaa cccaccagat aagagtacat tccgcctata tcggtcaccc tgtctacggc
     5401 gacgatgttt acggcaaagc tgtaaaggaa atcgaggggc agtgcctaca cgcaaagaaa
     5461 attggtttta tacaccctgc gaccaaggaa tatatggagt tcgacagcga attgcccgat
     5521 tattttcagg cggtgctgaa aaaactcaga gtagggaatt gacccacgga agtgatttta
     5581 tgtttgaaga tatatcaaaa gtcctgctta taacggatat ggacggaact tttctccccg
     5641 caagcaagat acctaccccg aaaactctcg ccgctgtcaa gcgttttgaa gaggctgggg
     5701 gcagattttc tatcgctaca ggcagagcca ttcaggcggc cagacagtat tttgacatgg
     5761 tgaatgtgaa ttgtcctatc ataatgtcaa acggtggaat ggtctacgat atccatacga
     5821 aagagcagat atataacgtc tttctgccaa gctttgcggc ggatatcgtc aaggagatac
     5881 ttgccgataa tccttatgtt ggctgtgaag tgcttactgt caacgaggtc tatgtgccaa
     5941 gcatgacccc tatggaaaag cgccataacg agatctgtaa gctcattccc gttcagtgtg
     6001 ccgttgacga gatacctgac aagaactggt acaaggtgct ttttgcagtt gagcctgagc
     6061 gtatggacga gcttataaac tatgtggcaa aaaaaggata caaaggtgta gattttgtgc
     6121 gttcaagtat cgagtattac gagatacttc cacagaatat atcaaaaggt acggctcttg
     6181 aggagctgag aaaagcttgt ggcatggagg attttacttt cgtggctgca ggcgactata
     6241 ataatgatat tgaacttctt catgcggctg acgtggcata ttgcccgtca aatgcggcag
     6301 aggaggtcaa aaaggttgcg gataaggtct ttgagcagtc ctgcgaggag gattttattg
     6361 ccgcagtcat agatgaaatt tttgaaaaac tgaagaaata atattttttt ggaggtcaaa
     6421 agtatggacg aaacattaaa gaaacagctt gagatcacag cctgcaaggt tcgtcttgga
     6481 atactcgagg gagtattcaa cgcaaagtcg ggtcatccgg gcggatcgct ctctatcgca
     6541 gatctgctca catatctcta ttttgccaag atgaacgtat atcctgataa gccggaagag
     6601 cctgagagag atagacttgt gctttcaaag ggtcacacag cacctgcact ctatgcaact
     6661 cttgcagaaa aaggcttttt tccaaaggag gagctgaagt ccctcagaca tatcggtgca
     6721 atgcttcagg gtcacccttg tatctctatc cctggtgtag atatgtcctc tggatccctt
     6781 ggacagggca tttctgttgc ctgtggtatg gcgctttcag gaaagctcac aagcgatacc
     6841 tataaggtgt atactatcct tggcgatggt gagatcgaag agggtcaggt atgggaggcc
     6901 gcaatgtttg ctgctcatta taagcttgat aacctcgttg ctgttgtgga taataacgga
     6961 cttcaaatcg acggaaagat agaggacgta atgtcgccat atcctatcgt tgacaagttt
     7021 gcagcatttg gctggcatgt tatcacagcc gacgctcacg attttgactc acttgaaaaa
     7081 gcattcaacg aggcagaaac agtatgcggt cagccttgcg ttatcgtaat gaagagtatc
     7141 aagggcaagg gcgtttcatt tatggaaaac aacgtttcat ggcatggttc agcacctaac
     7201 aaggagcagt atgagcaggc tgtggcagaa ataaacgaac agcttaaaag tctggagggt
     7261 taagcaatgg cagaattgaa gaagaaagct acaagagaaa gctacggcga ggctcttgca
     7321 gagcttggcg ataagtacga taacctctat gtttttgacg cagaccttgc tgctgcaaca
     7381 aaaacaggta tattcaagaa gaaattccct gacagatttt ttgactgcgg tatcgctgag
     7441 tcaaatatga tgggcgttgc agcaggtatg gctgcaacag gcaagatacc atttgcttct
     7501 acatttgcaa tgttcgccgc aggcagagca tttgagcagg taagaaactc tatcggttat
     7561 cctcacctca acgtgaagat aggtgcaaca cacgcaggta tctctgtagg cgaagacggt
     7621 gcaacacatc agtgcaacga ggatatcgct cttatgagaa ctatccctgg catggtggta
     7681 ataaaccctg ccgacgacgt tgaagcaaag gctgccgttg aagccgctat cctccatgaa
     7741 ggtccggttt atctccgttt cggcagactt gccacaccta ttttcaacga ccctgaaaca
     7801 tataagtttg agcttggcaa gggtgttaag ctccgtgacg gcacggatat agctatcgtt
     7861 gcaacaggtc ttatggttta tgaagctatc gaggctgcaa aaacactggc agccgagggt
     7921 atcgaagcaa cagttatcaa tatccacact atcaagccaa tcgacgagga tatcatcgtt
     7981 gaagccgcaa agaacacagg acttcttctt acagttgagg agcacagcgt tatcggcgga
     8041 cttggctcag ccgttgcaga cgttcttgca gcaagacacc ctgcaaagct tgttaagatc
     8101 ggcgttaatg acgagttcgg tcactcaggc ccggcagtag atctgctcaa ggagtacggt
     8161 ctgtgcgcag acaatatcgt tgcaaaggca agagatgctg ttaaggcaaa gtaataaaaa
     8221 caccaaacgg acggatatct tccgtccgtt tttttgtaca aaaaaagaca cgcttacgat
     8281 cttgtaaacg tgtctttttt atgcttttac agtgcctgca tgagtgtcga taatgttgtt
     8341 tatacatatg taaagataag gcttaagctc gccaatgtcc acaggctcgt tgtaaagtat
     8401 ggacgagtag caggttgagc ttacaagctc aactatcatg aatatcatga cctcaggctg
     8461 aacaatatca tatggagcgt cagctagcat agcgtcataa atgttgcgga agtcgatgtc
     8521 atcgtccttt ataggcgacg taagagcgtg cttgaaaacg ccccagctaa gattctttgc
     8581 aataaaagtc aaaagcgact tgttctcgtt tagctggttg atgatgttgt caacaataaa
     8641 aataattcgt tcgttaaagg tgagcttctt gccgctagca tttaatgcct cgatagcgtt
     8701 tctgaaaagc tgtgacgact tgtgagaaac aagcttgttt cttatgtcat atttgtcctt
     8761 gaaataaaga taaaacgtcc ctttcgccac accagcgttg ttcacgatgt ctgaaattga
     8821 ggtcttgctc actcccttgg tggtgaagag actgaatgcc gtgttaagca gagagtcctc
     8881 ctttaccttt ttattggtat caagcttgcc cattttaaca cgtccttcct tattataata
     8941 tatacaattt gtatgccaca catacacagg tttactgtgg ttgaccttaa atctattata
     9001 atacaaaacc gcctcaaagt caacggagca aaatgaccaa tagtcacttg tgaccgttgg
     9061 tcattttgtg caactctaca aaaagaatga ctaatggtca ttttactatt gactgttggt
     9121 catttttgat gtatactatg aattgtaaaa gaccgaaagg tcaaaaacta tgtgaaggga
     9181 atgacatttg atgcagaaat ttggcagagg agttgtaaag ctccgagtac cgatactcat
     9241 agtcagcgtt ctactgctga taccgtcaat attcggtttt ctcagcacaa gaataaacta
     9301 tgatattctt tcatacctcc cgtcagacat tgaaaccatg aaaggtcagg atataatgct
     9361 tgacgagttc ggcaagggtg gcttttcgct ggtaatgctt gacggcatgg aggataagga
     9421 cgttgaaaag gtcaaggaga agatcgaaaa ggtagaccac gtttgcgatg ttctctggta
     9481 tgacaccttg gcagacgttt cacttccaaa ggaagttctt cctgatgata tttacgattt
     9541 cttcaataca gataactcca caatgatggc tgtctttttt gacgaagcaa cctccgctga
     9601 cggctcactt gaagctgtca aggagataag aagcatcgca ggcgagcagt gtttcgtaag
     9661 cggtatgtcg tcagtcgttg aggatatcaa ggatcttaca atgcaggaag ctccgatgta
     9721 tgttgttatc gcagtaatac ttacaagtat catacttgca cttacaatgg attcgttcct
     9781 tattccactg ttctttatgc tcagcgtggg catggcgata gtctataaca tgggaacgaa
     9841 cttcatacaa ggcgagatat cgttcatcac cgaagctctt gcagccgtgc ttcagcttgc
     9901 ggtaacgata gactactcga tcttcctgtg gcatagctac aaagaggaga aagaaaagca
     9961 ccctggtgac aacaaagagg ctatggcagt tgccatcggc aaaactatca cctcagttgt
    10021 ttcaagctcc ataacaacag ttgcaggctt ccttgcactt tgcttcatga gctacgagct
    10081 tggcatggac atgggtattg taatggcaaa gggcgttgtt attggcgtta tctgctgcat
    10141 aacagttctc ccgtcaatga tacttgtctt cgataaggca cttgaaaaaa caatgcacaa
    10201 agaccttgtt ccaagccttg aaaagccttc aaagttcatt atcaagcacc atgcagcatt
    10261 tatcgtactc tttatagttg tacttatccc tgccgtatat ggtcagataa acaccaatgt
    10321 ctactataac ctgacagata ctcttccaaa ggatctcaac agtgttatcg caaacacaaa
    10381 gctagatgag gaatacaaca tggcaacgac ccatatgctc ctggttgacg ctgatatgca
    10441 gccaaaggaa gtaaactcta tgctcgacga aatgggcaag gttgatggtg taagcttctc
    10501 aatgtcactg gatactctta tcggtccgtc tatccctcgt gagatagttc ctgattcagt
    10561 aacaaagata ctcaagagcg acaaatggca gctcatgctg gtcggctctg aatataaggt
    10621 agcttctgat gaagagaatg cccagataga tgagcttagc aagatactta aatcctacga
    10681 taaggatgga annnnnnnnn naggatggaa tgcttatcgg tgaggcggcg gcaacaaaag
    10741 accttatcga cataacagat catgacttta aggtagtaaa tatagtttct atcgccgcta
    10801 tctttatcat cattcttatt gcactgcgtt cagtttcact gcctatcatt ctggtagccg
    10861 ttatcgaatt tgctatcaca gtaaacatgg gtgttccgtg ctttacgaat accaccatac
    10921 cgttcatagc gtcggtagta atcggtacta tccagcttgg tgcaacagtt gactacgcta
    10981 tccttatgac aacaagatat aagaccgagc gtaacgcagg caaagataag cacgaagctg
    11041 ttactatcgc actcaccact tcaatgaagt ctatcatggt cagcgcactt ggcttcttcg
    11101 cttcaacatt cggcgttggc gtatattcaa gcgtagatat gatctcacag ctttgtacgc
    11161 tcatgtcaag aggtgcaata atcagtatga taactgttat ctgcatactt ccttcaatgc
    11221 ttatgctgtt cgataaggta ataatcaata caacaatggg catgaagaaa aaagaaaata
    11281 agctttataa gcttcctgaa attcactaat tcgtaaagga gatatagtta tgaaaaagta
    11341 ttcaagagtt cttgcaggca tacttgccgt acttctttcc gcaggctgca caggtcttta
    11401 cgcagcagct cagtcagaca gctcttccgc tgccaacagc actgcctcaa caacaaagag
    11461 cgaatcagat aaaaaagata caaaagatac aaagaaagct gataagtctg acagtgatac
    11521 agaaaagcag gctaaggaag aaacagttta tgttatcact gacgcttccg gcaacaagac
    11581 aaaaacagtc gtttccaact ggctgaaaaa cccacagggc gtaaagaagc tggaggacgt
    11641 ttctgacctg acagatatcg agaatgttaa aacagacgag ggctttacag ccaatggtga
    11701 taagctcaca tgggataccg agggcggaga tatctactat aagggctata ccgataaggc
    11761 actcccggtt gatgtaaagg taacatatac ccttgacggc aaggagataa agcctgagga
    11821 cctcgcaggc aagagcggta agcttacagt aagatatgac tatacaaaca acgataagaa
    11881 aactgtcaag ataaacggca agaaaacaga tatctatgtt ccatacctta tggcaacaac
    11941 agctatcctt gatactaacg tttattcaaa cgttgaagta acaaatggca agctcatttc
    12001 agacggaagc agacagatac ttataggcgt tgcaatgcct ggccttacaa agagccttga
    12061 ccttcaggat aacgaagata tcgagatacc ggagtacgtt gagctcacag cagacgtaaa
    12121 gaacttcgag tcaacaactg ctctcacagt cgcaacatct gacatcttct caaaacttga
    12181 ccttagcgat ataggtgatg ctgacgatct tcaggataag cttgatgagc tttctgatgc
    12241 tacagacgag cttgtagacg gcacagcaga gctttacgca ggcataaaga agctgtccga
    12301 ttcctcagga acacttatca caggtatcga caagctttat tcaggttcta agtcacttga
    12361 cgatggtgca aagacactta acagcggtgc aacaaagctt cgtgacggag caaagaccct
    12421 tgattcttca acaggtgagc ttaagagatg tatctcaaaa gctaatagcg cttcaacagc
    12481 actcataggt gggttttatc aggtaaacag tggtatgtca accttgaaga acggctcatc
    12541 atcactttca agcggacttt cacaggtatc ctcaggcgca cagcaggtaa ataacggtgc
    12601 tgcaagcctt gccgcaggca caaataagct tgttacaggc gtaggcgacc tccaagcagg
    12661 ttctaaaaag cttgccgcag gcacaaagca gacctatgac ggttcaactg cactgaaaaa
    12721 aggcgttgca acacttaatg caggcgtttc cacactctat gaaaaggtgc agtcactccc
    12781 tgcaagcgtt gaacttcttt caaatggcat aacaaaggta gataacgcac ttacacagtc
    12841 tatcgcatac gaccagcagg tactcgcagg acttgagcag cttctcggca attacgaaga
    12901 aggctcagca gagtatacga gcataagcaa tatgatagca gccgtaaatg gctctatcca
    12961 gtatcagcag ggcgcacaga gcggccttgg cgatgtttca acaggtgtcg gtacaatggc
    13021 aaccgagggc aaacagcttg ttgacggcgt aactcagata tacaatggtc ttaacgataa
    13081 gaagaaaggt cttgtcgcag gcgttgataa tttgaataca ggacttaaaa cggtaaacga
    13141 gggtgcttct gcacttaaaa ccggtatcgg cacacttgct aagtccagca aggatcttaa
    13201 caatggtgca gctacactct ccgcaggcac aaagaacctc tccgcaggcg taagctcagc
    13261 acaggcaggc gcaagcaagc ttgataaggg cgttgatagc ctgaacacag gcgtatcaaa
    13321 gctttcaaac ggtgcaaaaa ctcttgacag cggtcttggt cagcttgaca acggcagcaa
    13381 gcagctaaaa gacggtactg caacactcta ttcatcactt gtagagcttg cacagggtac
    13441 aacaacactt tcaagcggca gtgcagagct gttcagcggt atcggtcagc tcaaagacgg
    13501 cagctcacag ctttcatctg gtgttaagaa gcttgaaagc ggtgcaaaga cccttaaaga
    13561 cggcatggct aagtataagg acgagggcat cggcaagctc cttgacatct acaacaacga
    13621 cctcaaggga cttatcgaca gacttgacgc tctcaagaca gcagccgagg attcaacaac
    13681 attctcaggc gcagccgatg gcgttgaaag ctcagttaag tatatttacg aaactgcggc
    13741 tattgaaaaa gcagacgagt aatttttact tcccacacat agcaaagacc cccttggaca
    13801 gaaatgtccg agggggtctt tgtatttata aacgtattat ctgtacaaaa aaacggacgg
    13861 aatctctccg cccgttttat atatcctact gtcttagata aacgccgttt tcttcgatgt
    13921 agtcctccat gtcctgcttg tattcaagat caagagaatc tttaggaagt gccttgagtg
    13981 ctttctgacc aatgtctttt ctgaaatgct ctccctcaaa ctttatcttg gaaacagcct
    14041 cataagactt gtccagtgcg ccctgaagcg tgtaagcatt gcaggtaaca cccagaacac
    14101 gaccgccgtt tgtgtagaac ttgccgtcct cgtacttagt gcctgcgtga taaataaacg
    14161 ctccgtctac ctgacccttg tcgtccatac cgaacatctc gatacccttt ttgtacttaa
    14221 caggatatcc tccgctcgcc ataacaacac aagtgcagta acccgacttc cagccgatct
    14281 tgacatcagc cagcctgtgt tcataaatag cctcaaatat ctcgcaaaga tcgccctcca
    14341 gcataggaag aacgacctgc gtttcagggt cgccaaaacg gcagttgtat tcaattacct
    14401 ttggaccatt aggagtgagc ataagtccga aatacaaaca gccctcaaaa gtcctgccct
    14461 cagcgttcat tgcattgata gtcggcagaa agatcttctc catgcactcc ttggcaatgt
    14521 cctcagtgta gtaagggtta ggtgcaacag tacccattcc gcctgtgttc agacccttgt
    14581 cgccgtccaa tgctctcttg tgatccattg aagagatcat aggaacaaca gtcttgccgt
    14641 cagtgaatga aagcacagaa acctcaggac ctgtgagata ttcctccaca acgatagtgg
    14701 aactgctctc accgaactgc ttgtcatcta tcattgagtg tatagcctcc acagcctctt
    14761 tctcactctc cgccagaata acgcccttgc caagtgcaag tccgtcagcc tttataacag
    14821 cagggtatgt gttctgttcc ttgacatatt ttatagcgtc ctcactgttt gtgaacacct
    14881 cgtatttggc tgtaggaata ttgtatttct tcataagatc cttagaaaag acctttgaac
    14941 cctcgattat agccgcattt ttcttaggac caaaggtctt gaaaccctca gcctgcaaag
    15001 cgtctaccat tccaagtaca agcggatcgt caggtgcaac agccaccatg tctactttaa
    15061 gcttctttgc cagagccaca acgccctcga tatctgttgc cgcaacatca aaacattctg
    15121 catagcgtga aatgccaccg tttccgggtg tgcagtaaag ctttcctacc ttaggggatt
    15181 ccgcaagctt cataacgatg gcgtgttcac gtccgcctcc gccaacaact aatatgtcca
    15241 ttttcagacc tcctcagtgt gtttttccaa ttataacaat accgcacacc tatgctgtgc
    15301 ggtatcgtca tttatttact tcgtatatta ttacttaagc tcaaagctgt caagaactgt
    15361 cttgaactcg tcagaaagct tgctgtaagc cttctcgctt gcaccatatg tgataacaac
    15421 aaggttgccg ttgtcagaag tgattatctg attgagcagt accttctgtg agccgatctc
    15481 gcctgttacg tcaacaatgt aagcgtcctt gcctgcaaac ttatcttcct tgtcgtttgt
    15541 aacagtgtag ccgtccatgg aattatatgt ttgcttgatc tggtcagcat aatcacttgc
    15601 tgtaagacca ttgagcagat cgtttgacat tgagataacg ttgatagaag cagactcaag
    15661 ctcagcatca tcgcctgcat cagcgtatgt gaacattacg tcaacaccag cggactcctg
    15721 cattgcccac ttctttgagt ctatcttgaa tgagtaaaga tcgcttgtgt aaacgtcatt
    15781 ctcatatgtg cctttagcct cgtcgccagt gcttgttgtg tcgtcagcag aagttgttgt
    15841 gtcagcagct gactcctcag gtgcagcagt tgtagttgtt gtgtcagcct tagactcaac
    15901 agcaggtgca gctgtagttg tagtagttgt tttagcctca gcctttgact cactgcttga
    15961 atcgcttgac tctgagcagc ctgtaactgc aaataccatt gacagtgcca tgagtgatgc
    16021 aagtaacttt ttcataataa atttctcctt tttttcttga cctttattgt tttgcattaa
    16081 tgcacaaaaa ttagtgatgg aacaatctta ttccagtgaa tgccattgcc atgttgtact
    16141 tgttgcaagt catgataaca tgatcgtccc tgatagatcc gcccggctcg gcaatgtaag
    16201 ccacaccgct ctttttagct ctctcgatgt tgtcaccgaa tgggaagaaa gcgtcagaac
    16261 caaggcaaac gcctgtgttc ttagcaagcc attccttctt ttcagcctca gtgagcacct
    16321 caggctttac cttgaatgtg ttctgccata cgccctcagc cagtacatca tcatgctcgt
    16381 cagaaatgta aacgtcgata gcgttatctc tgtcaggacg tcttatgttg tcaacgaact
    16441 gaagtcccag aacctttggg tgctgtctga gccaccagat gtcagccttg tttcctgcca
    16501 gtcttgtgca gtgtattctt gactgctgac ctgcaccgat accgatagcc tgaccgtcct
    16561 ttacatagca aacgctgtta gactgtgtgt atttaagtgt gataagtgag ataacaagat
    16621 cagttttctt gtcctcagga atctccttgt tgtctgtcac gatatttgtg agcatatcgt
    16681 tgtctatttt cagctcgttt cgaccctgtt caaaagttac gccgaaaacc tgctttctct
    16741 caataggtgc aggaacatag ttctcatcta tcttgatgat gttgtatgta ccctttctct
    16801 ttgacttgag tatctcaaga gcctcgtcag tgtagcccgg tgcaatgata ccgtcagaaa
    16861 cctctctctg gatcatcttt gcagtgcaag cgtcgcactc atcagaaagt gcgataaaat
    16921 cgccgtatga tgacattctg tcagcacctc ttgctctcgc ataagcgctt gcaagtgggg
    16981 taagctcgcc cagatcgtca acaaaatata tcttcctttc gacctcgctc aaaggcttgc
    17041 cgatagcagc gcccgcaggt gaaacgtgct tgaaagaagt tgccgcacac atacctgttg
    17101 cctttttaag ctctcttacc agctgccagc cgttgaatgc gtcaagcaga ttgatgtaac
    17161 ctggcttgcc gttgagtacc tcgataggga gatccttgcc atcctccata aatactcttg
    17221 acggcttctg attagggttg cagccgtatt tcaaaagcat ttcatttgcc ataattttta
    17281 cctcctgttt at
//
LOCUS       DAWEMR010000035        17262 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1382_length_17262_cov_10.621724,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000035 DAWEMR010000000
VERSION     DAWEMR010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17262)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17262)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17262
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1382_length_17262_cov_10.621724"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1578..1587
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9991..10000
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5174 a         3877 c         3572 g         4619 t
ORIGIN      
        1 tgatgatgaa tacacgcttt ctgttccaac atacaagtat gtttttttca caaatgatga
       61 taaagaagaa acaggcaaga gttacctcat tgttgacttg gagtataaaa accttgaaac
      121 aaaatctatc attcttaaag acagaatatc aagtaaagct atatatcagg ataaatatga
      181 atatgattta gatatatatg acaatggtgg ctatgatagc ggcattgcat ttgttttgga
      241 gaacgataat attgttaact gttatcatga aattcgtgaa gattatgatt tagagccact
      301 tgaaagcacc gagtttagct atgtttatag attgccagag gattatgacg gatcagacat
      361 aacagtacac atggatgttg ccaatgataa atatgatttc acatttgaga aaaatgaaaa
      421 tcatactgca cttgcacaag aatatctacg ataatatatt agcccctagt gtgtcacggt
      481 gacacactag gggtcatttt gcatttatat ataaaatata tatttttcgc cattaatttc
      541 cgcaaaagta ttgacaaatt gacaaaatgg tggtataatt aatgtataga ggaaatttag
      601 agggcataaa aaattgcacc ctctaaggct tataacctcc gccaagagat tataagccca
      661 gcagcacaga tgtaagaggc aactgtgagc tttcagagaa tgctatcctc gtgctctatt
      721 atagcacgtt ggaaatagat tgtcaagggc ttttcacgat gtcaaacatc tggaaagctc
      781 tttttgcttt tagctgctga ttcaactcat caaaaatgca gcttgaaaat cgaatatcgt
      841 aagtcctcaa ataagattga gatgaggtca actgatgtga aacacaagca caacaagaca
      901 ttgagttgtc tttaagtcgc tttgagccat gacgttcgtt actgttcaat cacgccgatc
      961 aggcattctt aacatttcag aacatgaaca cgagccagcc agtaacagtt aagctaactg
     1021 caagcctatg tgccgacgcc actatggatt aacatcagat gattcgccca agctccacaa
     1081 tgtgcagctg ccttgacaag gtctgagagc gaaacgaaac aaatgtcaca aaacatatct
     1141 tagcttgttc ctacaagctt tcatatatgc tcagcaagat actcaaagat acttgctgag
     1201 gaaagtccac atttttatat actgtgggtt ttcctttgtg agtatcgaaa atgattggtg
     1261 cgccaggagg gactcgaacc cccgacctac tggttcgtag ccagtcactc tatccagctg
     1321 agctactggc gcattcaact ctaatattat atcatatgca agcacccttg tcaacaagtt
     1381 aattgaattt ctccacttat tttacagaat gttcacatcg tttcttgtag cttcacacct
     1441 ttatccaccc tttaaatgcc attgtttttc acactttttg tcaatcaagt gtttgtaatc
     1501 atttgtaata ctttttcggc aaactgctct gtttttaacc ggaatttttg tagattattt
     1561 atcattctat gcccttgnnn nnnnnnnatg ttgtaataaa agaagtcaac cacaatatat
     1621 agtggttgag caggatagaa tgaaaggaag agtataggtg aagataataa aaagaagcgg
     1681 cgctgagaat acgttcgata aggaaaagat agaaaatgcc gttgcgaaag ctaatatcac
     1741 tgtggaggaa aaggataggc tctctgaggg agaaatagga gagatagcac agaatataga
     1801 ggacaaatgc tctgaaatga atagggctat ggacgttgaa actattcagg actgggtaga
     1861 agccgatatc atgcgccacg gcaaatatac agtggcaaag cattatatca cctaccgcta
     1921 tgagcgttct atcgtcagac aggctaatac tactgacaag cagatacttt ctcttcttaa
     1981 ctttgagaac gaggaagtca agcaggaaaa ttccaataag aaccctactg tcaattcagt
     2041 tcagagggat tatatggcag gtgaagtgag caaggatatc acaagaagat ttttgcttcc
     2101 tgacgatata gttgaagctc acgaaaaagg tctgatacat ttccacgacg ccgattattt
     2161 cgctcagcat atgcacaact gctgtcttgt aaatcttgag gatatgctcc agaacggcac
     2221 cgtcataagc gaggtcatga tagaaaagcc acacagcttc tctacagcct gcaatatcgc
     2281 tacacaatct atcgctcaga tagcttcttc acagtatggc ggacagagta tcacactttc
     2341 ccaccttgct ccgttcgttc agatatcccg tgataaatac cgccgtgagg taaaaaagga
     2401 gttcgcagag cttaatatcc ctgccgacga ggatactata aataaggtag ccgaaatgag
     2461 agtaaaggct gagatagttc agggcgttca gatgatacag tatcaggtca tcactcttat
     2521 gacaacaaat ggtcaagcac ctttcgttac tgttttcatg taccttgacg aggtgcctga
     2581 gggtcagaca agagatgacc ttgcggctat catagaggaa atgctcagac agcgtatcca
     2641 aggcgtaaag aacgaaaagg gcgtctatat aacacctgcg tttcctaagc taatatatgt
     2701 ccttgaagag gacaacataa gagaaggctc aaaatattgg gagcttacaa agcttgctgc
     2761 aaagtgtacc gcaaagagaa tggtgcctga ctatataagc gaaaagaaaa tgaaggagct
     2821 taaggtagac aagaacggaa atggtcagtg ctacccttgc atgggctgca gaagcttcct
     2881 tacaacatat cttgacgaaa acggcaagcc taaatattac ggcagattca atcagggcgt
     2941 tgttacaata aaccttgtgg acgttgcctg ctcgtcatat aaggatatgg ataagttctg
     3001 gttgatattt gatgaaagac ttgaactttg cagacgtgcg cttatgctcc gtcacgaaag
     3061 acttaaaggc actccgtcag acgttgcgcc tatcctttgg cagaacggtg cattggcaag
     3121 gcttaaaaag ggcgaaacta tcgacaagct cctgttcggc ggatattcca ccatatcact
     3181 tggctatgca ggtctttgcg aatgtgtaag atacatgacg ggcaagtcac acacagaccc
     3241 ttcagcaacg ccttttgcac ttgaagttat gcagcacctt aatgacgcct gcgcaaagtg
     3301 gagagcagaa acaaacatag atttcagcct ttacggcacg ccattggagt ccacaacata
     3361 caaatttgca agatgtttgc agaagcgttt cggtgttatc gagggtgtaa cagacagaaa
     3421 ctacatcaca aacagctatc atatccatgt taccgagaac atcgacgcat ttgacaagct
     3481 cacctttgag tcacagttcc aggctctctc accaggagga gctatcagct atgtggaagt
     3541 gccgaatatg cagaacaaca tagaggctgt tcttgcagtt atgcagcata tttacgacaa
     3601 catcatgtat gcagagctta acacaaagag cgactactgt cagaagtgcg gttttgacgg
     3661 tgagataaag atagtagaag atgacggcaa gcttgtatgg gagtgtccga actgcggcaa
     3721 cagagatcag aacactctga acgttgcaag gcgcacctgc ggctacatcg gcacgcagtt
     3781 ctggaatcag ggcaggactc aagagataaa ggaaagagtt ttgcatttgt gatttttgta
     3841 aaatcatttt gtaaaattac aattcataat gagatatgac aattcataat gagatattaa
     3901 cagcttgtac acaaaacaca attaaaaacc caaaaatttt ttctagcaaa atcgaaaatt
     3961 tttgaacatt atagccgtta aaactcaaaa gttcaaaaat caaaactcgt caaaaaaatg
     4021 aacattatag caaggaaaac tcaaaaactc aaaactgcaa aatccgaaaa taatgaacat
     4081 tattattgac ataaaacagc aaacagcata taactatcgg ggcaatacaa aagcgtattg
     4141 ccctgcgttg tatgtatgaa aggagcggaa aatgaattat tgtgagataa agaaaaccga
     4201 tatcgccaat ggatcaggag tgagggtcac gctgttcgta tcaggttgca gacaccactg
     4261 caaaggctgt tttcagcctg acacttggaa ctttgactac ggcaagcctt tcacagatac
     4321 caccgcagac gagataatta acgccctctc aaagggctat ataaaaggac ttacgctctt
     4381 aggcggcgaa cctatggagc ctgaaaacca gcctgagctt acaaaactcc tccgccgtgt
     4441 gaaaacagaa cttcccgaca aggacgtgtg gtgctacaca ggctgtaccc ttgaaactga
     4501 cctgcttgca ggctcaaagt ccccatacag aacacagtac acagacgaaa tgctgagcct
     4561 tatcgactac ctcgttgacg gcgaatttgt tctcgaaaag aaaaacatct cactgaaatt
     4621 cagaggatct gaaaaccaga ggatacttca taaagaggac ggcatttggc tgccttgcga
     4681 agatatgtaa aacaaaaccg ctccccgtga catcacgaag agcggttttt ttatacaaat
     4741 tgtaggggga cggcgtcctc gacgtcccat ttattgacca tgtgtcatgt tcttgacctt
     4801 acttctcaga ctttgtaaag aatatcgcct ttataagctg gataacgagc atagagccaa
     4861 agcttagaat gtatatccat gcaaactgca tacctgtcat gtctgctatc ttgaaaagtc
     4921 ccttgagtgg tggaacgagc agaacggagt ttaaaaatac cattccaaga ccgaacgcca
     4981 taagaccgaa cttgttgttg aaaaagtcct ttgagaagat aacaggacct tttttcttgc
     5041 aggagaatcc gtggaacaat cttgcagagc aaagcactgc gaatgccatc gtcataccaa
     5101 gagctgcgct tgtcttgttt cccataagga aagccaaagc tactgctatc gctataacta
     5161 cgccatacaa tgctatctcg cccaggaaag gacgtgtgag gatagattcg tttgcgtttc
     5221 ttggcttgcg tttcatgacc tcttcggagt gaggttcaag acccaaaccg atagcaggaa
     5281 gcgagtctgt aagcagatta ataaacagca aatgtattgc cgcaaacgga acaggaagtc
     5341 caagcagtga gttgaaaagc accacaagaa ttgccgcaaa gttgcccgaa agcaggaaga
     5401 gtatagcctt tttaatgttc tcgtaaatgt ttctgccgtt tcttatggcc tttactatag
     5461 ttgcaaagtt atcgtctgca agcaccattg aggcagcgtc ctttgaaacc tcagttcctg
     5521 tgatgcccat tgcaacgccc acatcagcct gctttaacgc aggtgcgtcg ttcactccgt
     5581 cgcctgtcat ggaaactata cagccgtttg cctgccaagc ctttacgatc cttattttat
     5641 gctcaggtgt aacacgagcg taaacagcct tgtcctttac aaagtcaaca agctcttcgt
     5701 ctgaataagc gtcaagctcg tgaccctcaa cagcctttga gttgtcgtca agaataccta
     5761 tctcacgggc aatagccgaa gccgtaacga tatggtcgcc tgttatcatg actggcttta
     5821 tgcctgcctt gcggcattcc gcaacagccg cctttgattc ctctctcgga ggatccatca
     5881 tagcaataag accgacaaac tccaatccgt cctcgtcctc tggacatacg gtatctttgt
     5941 cgaatttttt ctctgcaaat gcaagtatac gcagaccttt ttccgaaagc tctgccacac
     6001 gctgggttat aacagccttt tcttcgtcgc ttgaagttat cctgtttata agaacgtcag
     6061 ccgcaccctt tacatatagc accttctcgc cgtcgataac gtgcagagtt gacataagct
     6121 ttctgtctga atcaaatggt atctcagaaa ttctagggag atcttctctc accttgtcag
     6181 tgtctatacc aagctttgtg ccgaagttga taagagccgt ttctgtaggg tcgcctatct
     6241 caacgccgtc cttacagctt gaatcgttgc aaaggatcat ggctcttgtc atagtcttga
     6301 ccttttcatc gtcaagatca acagcgtcag tgtcgattat cctgccgtct accattattt
     6361 ttctaactgt catcttgttc tgagtaagag taccagtctt gtcagagcat attacagaaa
     6421 cgctgcccaa gccctcaact gcctgaagct ttcttatgat agcgttttcc tttgacattt
     6481 tctgtgtgcc gaaagaaagc acaatagtaa cgatagagct gagagcctca ggaatagccg
     6541 caacagcaag tgcgatagcg aacatgaacg aatccataag ctcgccgcca cgaagcatac
     6601 taagtccgaa cactactgca cagacaataa gtatagcaat ggaaagcttc ttgccaaact
     6661 cgtcaagcgt gttctgaaga ggcgtttttc tctctgatgc gttttggata agcgaagcta
     6721 tcttgcccac ctcagtgtcc ataccaacct cagtgacaag catttttcct ctgccgtatg
     6781 tgacaaaaga acctgaataa accatgttag cacgctcagc aagaggcttt tcgccctcta
     6841 tatcgctcat atccttgtcg atattaacgc tctcgcctgt gagtgccgac tcgttcacct
     6901 gaacagacgc acattctatc agcctgccgt ctgcacatat ctggtcgcct gcttcgataa
     6961 gaagaatgtc gcccactgcg atctcctcag aggggataat gaccttttcg ccgttacgaa
     7021 gcgccttagc cgttggtgca gaaagctttt taaggttcgt cagtgacttt tcagccttga
     7081 cggtctgaac tgtacccaaa atagcgttca ttgtgataac aacgagaatt actgcacagc
     7141 tctcaacatc tttcatgaaa gctgaaacta tcgcagcaat aatgagtata agcacaagaa
     7201 agtccttata ctgctctaaa aaaatcacgg gaatggactt tttcttgccc tctgtgataa
     7261 catttcgtcc gaacttttcg cagtttttca ctgcctgctc ggttgaaaga ccattttcac
     7321 ctgatgaaaa ctcagtgtaa agctggtcaa tttttttttg atactgcttc ataaaatacc
     7381 acctttttta ttttttcctg taaacattgc cgatataccc ttccaagcct ccttttctca
     7441 taggctcagt aaaataaacg cataaaaaat cggcaagacg tttattgggt aaactgcgcc
     7501 tgtggcaatt taatcaataa aagtctcgcc gtttagatat gcagcggtcc ctcatcggaa
     7561 cgtattgacg caaacataaa cggatctctc cgccagctac tctcttttac tatgaggtta
     7621 ttataaccta tatttttgat tttgtcaata gttttttata tattttattg taaattcgca
     7681 actcatttgc ttgatctcat tttcaaacac tgcccttgat tattattcca aaaggtgcta
     7741 taatagcatt ggtgataata atgaaaagga aaatacttgt aggtctgctt accgccgtta
     7801 tctttttggc ttgcgccctt gtgataaata taactccaaa ggtggagaaa ggttcagttg
     7861 cgctgacctg tgacggaagc aaatatgagc ttcccggcac tgaaaagaca cgatactgca
     7921 acggcaagag cgagagcctt tccgctgaag agagctttga agatctgctc tcctctgtac
     7981 cgtcatttaa tataaaggca gatatagata aggacggcaa tgttaccctt aaaaccccta
     8041 tgtccgtaga agtcacaggg gacaggctcg gtgacgtgct ttacactgtc tacagctatg
     8101 acggaaaggt gcttgccaag gagtccaaaa agctggagct tccaaaaaag gatattgacg
     8161 gctgtcttgt gaaaataaaa attacatggg gcaagaaaga cacaagctat cttgaagaag
     8221 attactggtt tgccgcaatg tacaataccg aaagataaac ataacatcaa aatgtagggg
     8281 cggcctctgg tcgctcactt tctttctgta aactttcaga caacaaaacg tcggacgaat
     8341 ttgaaaacgt ccgacgtttt gttctttaac atatttgaaa tcctattatg cccgccatac
     8401 aaataacgct gccgataaat ctcagctttg tgataggctc ttttcttatg agcgctataa
     8461 caaacagcga acacagtatc ataggctgaa taaatgacgc actcgcaagg ctcacagcaa
     8521 taacagcgtt ctccgcaagc agacctgcca cattcggaat ctttgtaagc acaaccaccc
     8581 atgcgccctt ttgatttttc ttgaatatct ccaaaggctt cgcccttggc agaagtatga
     8641 tagccataag tataagcgca aagaacagcg ccatggtcga ggatatgtaa ttctccgacg
     8701 ctcttacaac tatgccgtag ccgtatcttg ccaaaagata gaacaccaga ggaacaacta
     8761 tcctgcggta atttatattg ccgctgtccg ttctgcctga ttttgcaata aaaacaagtc
     8821 ctgcaacagt aagcacgata aaaagaaact tgaaaatgct cggcttttca ccaaggaaaa
     8881 tatccgttgc ataggacatg aaaagggtga taccaagcca agccttaagc tcaaaagctg
     8941 atatttcatc aaggataata gccgaaagct taaattcaag gatcttcgac aagcacagca
     9001 gacctatcgc cgcaaaggat tgccagctaa gcgtaatagt cctgtcgagg aaaggcagac
     9061 agcaagccat gaaaacagcc gttgccgccg ccattaagaa tccaagctca tcgccgttga
     9121 attttgccgt tgaaacagcg tacttgtcac taagggaaca tatcgtgtag catacaacta
     9181 caagtataag caaaaacatt atttttcttc ttccttactc tatgattttc taagacccgt
     9241 atgctgtgaa acatacgaaa taatgagcga cccaaggtcg cccctacgca aagccgaatt
     9301 atcagttctt tttatttttc acaagccata ctaccgccaa aataccaagt atcactgaga
     9361 acagagtagc aatggccaga catatccagc ttgcatactg actttgtata aattcgttct
     9421 gcgattggct tcctataagg gtatctaccc ccgattgcga gcctaacgct gagaacagta
     9481 ttataaatgc cattctcaca ttcattacgc aggctataac gctgtaaata tgcactatac
     9541 cctgaaaaag acacactccg cagtcgtcct ttacatagct tttcactcca agcaccgcac
     9601 ataccaccga cactgccgtg tagataatga acgccgcaaa aagcactatc gttggcactt
     9661 ttgccccctc ggttttcact atcatgctca taagcccagc catacacccc atgaacacag
     9721 ccgctgccgc aagccctgca acagaccatt ttctgtataa aagttccgac ttttttatct
     9781 gctctttctt tatctgctta ggcattttct tcctccgcct tcttgctttc aagctcctcc
     9841 tcagatatct tgaagcgtat cctgtctatt ttctgctcgt cctccatttt cactgtcatc
     9901 tcaaaaggcg gacagcttat aacttcattc tgctcaggaa gtctgtcaag catatccatt
     9961 atccagccac cgagagatgt tctttcggtt nnnnnnnnnn ctccggcagt cctgtcctct
    10021 caagaaaatc cgataccgaa agctccgccg acgcttcgta aacaccgtcg cttattttca
    10081 caagggaggt atcctcctcg tcgctttcat catagatctc gcctacaagc tcctctatga
    10141 tatcctcaag ggtacaaatg ccctccgtgc cgccgtattg gtcgagcacc accgccatat
    10201 gcaccttttt gcgctgcatc tgcttcaaga tctcagagat cttgcggttt tcggttatgt
    10261 aaagtggctt gttcattata aggcttatgt ctgtcttgcc cttgagatac atttcaaaaa
    10321 agtcagactg atggataagt cccacaatgt ggtctaagtc cttgtcatac actgggagcc
    10381 ttgaaaactt tgtctgcaca aagcgttttt ttatgctctc catatcctca tgaagctcaa
    10441 caccctcgat atttactctc ggcacaagta tctcgcttat ggttatctcg tcaaaatcaa
    10501 gtgccgaacg cacaagctcc gactcctgct cttcaagtac gccctcgtcc tgaatctcgt
    10561 ctatgatata tttaagctct tcctcagtaa cagacggctc gctgttcttg ttgcccacaa
    10621 gctttgaaac gccgcttttt atgcccataa agatagccgt tataggcgtg atgatgaaca
    10681 tgaatgcgga aagcggagcc gccataagaa tagaaaaccg ctcagaattt tccttcgcaa
    10741 ggctcttagg caagatctca ccaaaaataa gcacaagcac cgtcatgact acagtagcca
    10801 gacccacgct tccctttccg aacttttccg taaaaagcac cgttgcaagt gaagatgaag
    10861 aaatgttcac cacgttgttt ccaacaagta tagcagtgag tgccttgtca aaattatcgc
    10921 atatgttcat tgccttcttt gcagacttgt ttccgtcgtc tgcaagtttt tttagcctta
    10981 tcctgttgca tgaagaaaac gctgtctccg ttgcagaaca aatagcagac agcataagaa
    11041 gcacagcgat gataacaact ttcataaaat aaaattatcc tttcaggtca gaatataaat
    11101 ttgctgccct agttataaaa atagcactaa aggcagtcat gaaaactgac tacatatagt
    11161 tatcataaca tattttttaa ccaattgcaa cagtattgtt gaaaaaacga tgtaaaagag
    11221 ttataatatt ctttgtgttc ataatattca tatgcacaaa aggagataac agaaatggca
    11281 aagaaagata aagacatttt cgacaaaata atggactgga agatattcgg ctggctcaga
    11341 cccttttacg tcaaaaacaa ggaaatgctt ttgtatctgt ttttcggcgt actcaccacc
    11401 gcagtcagct ttgtgaccgc aggcatctca aaagtgcttt tggagcaggc aggcataggc
    11461 aagggcggtg tttctaccac aagcaccgtc atatcatgga tatgtgcagt aacattcgca
    11521 tacataacca acaggatatg ggttttcgag tctgaagcag agggcaaaaa ggcgataatc
    11581 tccgaagcgg cttcatttta cggcggaagg atattcactc ttctcgtaga aatgttcatg
    11641 atgtggctcg gctactcact tctcagcttc aactattggg taacaaagat agtggcaaac
    11701 gttgttgtgc tgatacttaa ctatgtcatc agcaagcttg tggtcttcag aaagaaataa
    11761 gcatacaaaa aaagctgccg gcaaaaaatg ctgacagctt tttattttgc cccagttgcc
    11821 acgttgcaac cgactaatct ctgtacataa gcgtgaccgc catgccgttt atcacgccca
    11881 ctacaactcc gcacacaatg aaaaacacct tgataggcag gtcaagcaga agcgagataa
    11941 gcccgaacac gataacgaac gccattgctc cgcctatctg cacaagaaac tttcttttca
    12001 caagcacagg aaaacgctca gcaagctttg ccgtcttttc gcctttgtgg tcaaacagcc
    12061 tgtataaaag ctgagtcagt gccattggta ttccgataaa taaaattgct gttataagtt
    12121 tgtccatttt aaattcctcc atttttcact taaattatag ctttttttgt atcaaaaaag
    12181 gctgcccaag acgcagttcg tccgtgggca gcctcattgc tgcatataaa atttctttta
    12241 tcaacaatta aagaactctt acgccaacaa cgccctcgat agacttgaaa gcgtcagcgt
    12301 caacgtcgcc tgtaacatcg agcattgtgt aagcccagtc tttcttagac ttgtttacaa
    12361 ggttctcgat atttgcgccc ttgtcagata caacagatgt gatctgtgca ataagtgcag
    12421 gaacgttctt gtgaagcacg caaacaaggt ggtcgcctgt tttagcaagc tctgcattag
    12481 ggaagtttac agaattcttg atagttccct tctcgatata gtcgataagc tcgtgagctg
    12541 ccattgttgc gcagttgtcc tcagactcag gtgtggaagc gccaaggtgt ggaagaacga
    12601 taacattctc ctcgccaaga acaacatcat ctgcaaagtc tgttacatac tttgcaacct
    12661 tgccgtcctt gatagccttt acaactgccg cactgttgat aagctcacct cttgcaaggt
    12721 tgataagacg aacgccgtcc ttcatcattg ctatctgagc ttcgtcgata gtgttctttg
    12781 tgtcaggtgt ataaggaacg tggatagtga tataatcgct gttcttgtaa atatcattga
    12841 tgtcagctgt tacctttaca gcaggatcaa gctggatagc tgcgtttact gaaaggaatg
    12901 ggtcatagcc gataacgtcc atgccaagtg caacggctgc gtttgcgatc tttccgccga
    12961 tagcaccaag accaattaca ccaagtgtct tgcccagtat ctcaggacct gcgaacttag
    13021 acttgccgcc ctcaactgtc tttggagcgt caggagtgcc cttaagtgaa gcagcccatg
    13081 cagcagcctc tgttatcttt cttgaagcaa gaagaagtgc acaaatagca agctccttaa
    13141 cagcgtttga gtttgcgcca ggtgtgttga atacaacgat tccctgctct gcgcacttct
    13201 caactggaat attgtttacg ccagcacctg ctcttgcaat agcaagcagg ctctcaggca
    13261 tttccatatc gtgcatcttt gctgaacgta ccattatagc ggtaggattt tcagcattgt
    13321 cgcttactgt gtacttagcc ttgtcaaaga tatcagtacc gcaggtagcg atcttattta
    13381 atgtctttat ctcatacatt gtaaattacc ctctcttata ataaggttac gcttatgcgt
    13441 tctcagcctc gaacttcttc atgaactcaa caagcttttc aacgccctcg ataggcattg
    13501 cgttgtagat agaagctctc ataccgccaa cagttctgtg acccttgagg tttacaaagc
    13561 ctgctgctgt agcctcagca acgaacttct tgtcaagctc agcgtcgcct gttacgaatg
    13621 gaacgttcat aagagatctg tcctcaggaa caacagtgcc cttgaagagc ttgctctggt
    13681 caagataatc atagagtatc ttagcctttt tctcgttgtg agccttcata gcctcaagac
    13741 cgcccatttt ctttatccac ttgaatacct tgccgcagat gtagatgcca tagcaaggag
    13801 gtgtattgta aagagagtca gcgtcagcct gagttttcca cttgagcatt gtaggtgttc
    13861 cctcgagaac atcgtcagtg ataagatctt ctctgatgat agcaataaca acgccggcag
    13921 gaccaacgtt cttctgaacg ccgccgtaaa taacgccgta ctttgttacg tcaacaggct
    13981 cagacaggaa gcaggaagaa acgtctgcaa caagtgtgtg accctttgtg ttaggcagag
    14041 tcttgtactt tgtaccatag atagtattgt tttcgcagat ataaacatag tcagcgtcct
    14101 ctgggatatc gaggtctgag caatcaggga tataagagaa agtcttgtca gcagaagaag
    14161 ccacagcaac agcctcgccg tatttctgag cctcctgata agccttctta gcccactgac
    14221 ctgtgattat gtaagctgcc ttcttgttct tcataaggtt cataggaacg gctgagaact
    14281 gctgagaagc accgccctga aggaacagca ccttatagtt atcagggata cccataagat
    14341 ctctgatgtc cttttcagct tccttgatga tgtcatcgaa cgccttggaa cggtggctca
    14401 tctccattac gctcatacct gtgcccttat aatcgagcat ttcatcggca gcttccttaa
    14461 gcacttcctc agggagtaca gcaggacctg cgctgaagtt atatactcta cccattgtta
    14521 aaccctccat ataaattagt ttcattattt ataaagacta taaatatata aattaataat
    14581 attattatat gccttttata aaaaaaagtc aagggctgtc atataaaaat actcactcgt
    14641 cataagtttt tgtacatatc agcacataaa gcagccctga aaacgtgcat tttttcacct
    14701 tacctatgcc gttatatata ataagcactg aaaagcatga aaaatcagaa taatactaaa
    14761 gcgttaaaaa gaggttaaaa tttttggtat tccttgaaat ctccatattt gtgtagtata
    14821 atgtaatcaa taaaatgcga cagttgtaaa aaaatcgggg aacaattgtc actctcaggg
    14881 aaagaggata tatatgcaaa agatatttta tgtttcaaga aatgaggaca aagcccatga
    14941 tggaaaagcc ccggatatgg acagatttca gcgagttgaa aagctcaaca gtctgatcgc
    15001 ggcaggctgg gctataaagg aaatgaaaag cgaaaacaac agcacattct ttgtgcttga
    15061 gaaagcagac tagactttaa atggacgata tcgagccgta tcctgtcaat aacacagaca
    15121 cgttgttttg taggggacgg cgccctcgac gtcccgccca ctcaccagct ttttcaacaa
    15181 atttacagga cggcagcccc accgtccttt ttttatgtgc agataaattt tgcgaaaacg
    15241 ttttatgggt attgcatttc agagagaaat attgtataat taatgtaatc gttttaatga
    15301 gtacaaatga tactatacat attataaagg agtaaaaaaa tatggcttat gtaatcggcg
    15361 tagactgcgg cacaagcggc actaagacgg tacttttcga cgaaaagggt actgttattt
    15421 cttctgtaac tattgaatat cctatgtatc agcctaaaaa cggctatgca gagcaagacc
    15481 ctgctgattg ggcaaacgca atgataaaca ctatcaaggc tgttatgacc aaaagcggcg
    15541 taaataaaga ggacgttgca ggtattggta tctctggaca gatgcacgga cttgttatgc
    15601 tggataagga caacaacgtt cttagaaagt ccataatatg gtgcgatcag agaactgccg
    15661 cagaagttga agaaatgaac gaaaagctag gcagagaaaa gctcatcaag ataacagcaa
    15721 accctgccct cacaggctgg acagctgcaa aaattctttg ggtaaagaac aacgagcctg
    15781 acatatacga aaagtgcaga cacattcttc tgccaaaaga ctacttgaga tttatcctca
    15841 caggcgaata tgcaacagag gtttccgacg caagcggtat gcagcttctt gacgtgccaa
    15901 accgctgctg gtcaaaggaa gtctgcgata cgcttggcat tgatatgtca atgctcggca
    15961 aggtgtacga gtcatgcgag gtaacaggca aggtcacaaa gaaaatggct gagcttacag
    16021 gacttaaaga gggtactata gtagtaggcg gagcaggcga caatgccgct gcggctatcg
    16081 gaacaggcgt tgcagaggac ggtaaggcgt tcacaactat cggaacatca ggcgtcgtat
    16141 tcgcacacac ttcttctatc tctatcgacc caaagggcag agttcacacc tgctgtgcag
    16201 cagtaccgaa cgcatggcac gttatgggtg ttacacaagg cgcaggactt tcgctgaaat
    16261 ggtttaggga taatttctgc aatgcagaga aagaaacagc aaagtgcatg ggcgttgacg
    16321 aatattatct catggataag gaagcagaga aagtgcctgt tggtgcaaac agacttctct
    16381 atctgccata tctcatgggc gaaaggacac cgcatcttga ccctgacgca agaggagtat
    16441 tcttcggact ttccgcaatg cacacaaagc gtgatatgct gagagcagta atggagggcg
    16501 tatcatactc cctgagagat tgcgttgagg tattcagaga aatggatatc aacgtatccg
    16561 acatgatggc atgcggcggc ggcggaagct caccgctgtg gagatcaatg ctcgcagatc
    16621 tttacaactg ccctgttaaa acagcctcat caaaggaagg cccagccctt ggcgtagcac
    16681 ttcttgcagc aacaggcgca ggcatttact catcagtacc ggaagcttgt aaggcagtag
    16741 taaagaccga caaggtacag cagcctgaag cagagcgagt acctgagtat gagaaatact
    16801 acaagcttta cacagagatc tatcctgcac tgaaagtaga atttgcaaag cttgcgaaga
    16861 tgtaatataa aaccaaaagc tccgatcatt tcggagcttt tttgtattag aatattttga
    16921 caactaaaac ggctctccac aatagcggaa agccgttttt acatattggt cggagtgacc
    16981 tgatttgaac aggcgacctc taccacccca aggtagcgcg ctaccaatct gcgccacacc
    17041 ccgatatcgt atatattata cccgatttgg atacaatagt caagagtttt cagtcaaaat
    17101 aaaaaaattg caaaaaaggt attgacattc acattcattt gtgatataat aaataagcac
    17161 tcaagagaga gcacaaaaaa atatcgcggg atggagcagt tcggtagctc gtcgggctca
    17221 taacccgaag gtcgttggtt caaatccagc tcccgcaacc ag
//
LOCUS       DAWEMR010000036        17093 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1404_length_17093_cov_11.050593,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000036 DAWEMR010000000
VERSION     DAWEMR010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17093)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1404_length_17093_cov_11.050593"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7203..7212
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4685 a         3562 c         3934 g         4902 t
ORIGIN      
        1 tgtgtatttt tatgccaaaa tagttttctt aaactaattt gttttttgtt tttaattaac
       61 aaaataaata acttaacaaa tatagtatag caaatattag tatagattac attaactaaa
      121 cgtgaatttt atatttatat tatattaata cgaaacaaaa tgcacaaaaa gagtttaaaa
      181 aatcgtacaa aaagtaactg ccaaaaggtt tgcaattttg gtgaaaatat tgtataataa
      241 ataagtataa gatgtcaggc ataaaaagcc gatatcagat catcatgcca ataaaaaggg
      301 ggagatctta tgggtctgtt cagcagaaaa agctccgagc ccaaaacatc gacacagcct
      361 aaaaagcagc agataaatgc tgatgatata tggaacacgc ctgtgaagag agcaggcgga
      421 gatacggctt ttgtgaagga gtccagcagc gataaggtac acgctgagaa gataaccgat
      481 accatagagc cggaaactat ccgcagcaag atggagcagc ttgaaaaaga gctggctgaa
      541 aagaaaaacg atcccgtaaa gacctatgcc gactacgacg tgaacccggt aggacaggct
      601 gagataaccg atgcccagag cgagtatgaa aagctttatg aggaggaaca cgcaagattt
      661 gtagcttctc ataaggagga tataagcgtg gcgtccgttg aaggtatcga ctcaaagctt
      721 gaggagatat ataggataca tgacgagaaa gtggctcagg agaagcgtga tacaggcaat
      781 atcgaagcca taagtgcaga aaccacagcc gcaaaggtgg ctgagcttcc ttatgcaaaa
      841 aagcctgagg actatgcaga gtataaggat ataaagggaa tatccgtcga tgaggctctt
      901 gtggagggtc ttggctctgt tgaccacagc gaggacgacg acgctattgg ctcagtatcg
      961 gaagagtttt tggcgcagaa ggtgaaagaa tttaacgaaa aatatggcag atgatcgggg
     1021 taaaggcagt cattaggggt attgccagaa taaattgaat tggagagata aaattgaaga
     1081 tcaataccga taaccctatc ataaagtttt caggcaaagg caagccgttt cagtatgata
     1141 agttgcttta tgctacatta aatgagtata tacttgatta taagaatgca agacttgata
     1201 agcttaccga tcaggacgct tctatatgcc ttgcgagaat aataagaaaa atggaagtca
     1261 atgacgttcc tgtccagcag ttttttcatg aagagcttga aaagtggagc gagcatacca
     1321 actatgagaa gatactcaga ctttgcgagc ttatggcaaa ggatatcttc ggctgttttg
     1381 ataagaacag agatgacggc aacggcggct tctataaaac agacaggctc tattgcgtaa
     1441 ataatgacgg tgaaagagat tatatcgttt gtgacgaggt tgagaaaaag ggacttttca
     1501 agaaagttcc aactcctgtc acattgtatt tcaatgatct tatggagaaa aataaacgtg
     1561 gcgagctgcc aaaatccaag taaattttgt attaacagat aataaggaga gtattaacat
     1621 ggtaaatata gaaatggcaa acggaaagaa tatcaagatc gagctttatc ctgacgttgc
     1681 acctatcaca gttgcaaatt ttgagaagct tgttaaagag ggcttttatg acggacttat
     1741 ttttcataga gttatctcag gctttatgat acagggcggc gacccgacgg gaacaggcat
     1801 gggcggtgca aaagagaaga taaagggcga gttccttgcc aatggtgttc gtaacgacct
     1861 caagcataca agaggcgtta tctcaatggc acgttcagca atgccaaatt cagcaagctc
     1921 acagttcttt atcatgcatc aggacgctcc tcatcttaac ggtcagtatg cagcattcgg
     1981 caaggtagtc gagggcatgg acgttgttga cgagatcgca gcagttgacg taaatttcaa
     2041 cgataagccg cttgtagatc aggtcatgaa gaaagtaact cttattgagg actaataaaa
     2101 aggaaaatca tgggctgcgg tctttatggc ggcagccctt gtttacgtca aaaacaatat
     2161 caggagagta aataatggat ttcaaaaaag taacagtggg attttttgca gcggtcatgg
     2221 caatgacctc agtgtgtttc gctgatcccg aaacgcctgt gacaaccgat gtgccagcca
     2281 cagatcctgt accggaaaca accactaccg ctcagcctca gtgggaggac actgaaacga
     2341 ctacaactac cacagctcag acatgggaaa ccaccacatg gggaactacc acatcttatt
     2401 cctatgttcc ggacactccg acttctacgc cgacagttgt aaagcttgtg gtcagcgagg
     2461 taaaggataa aaagttcaca gcgaaaataa atatccagtg cgaccacctt atctcaaacg
     2521 cctctatctc cgtgggctat gacgatagcc ttgtagagtt tgaaaagtgc gaaaccaatg
     2581 ataaagcagg gggcatggca gtagacaatg cctttgctgg taagtttgta tataactatg
     2641 tgaacgcaga cggcacagac tttgacggtg attatgcgac cctccatttc aaagtcgcag
     2701 acgagaagct cacctcaaca gtgctttatc tcacagtaaa ttcccttgat gacgaaaacc
     2761 tcaatgccat atcaaatcag gtagagaacg gtatcgtcaa gtatcaggac gctgcagacg
     2821 ttaagcagga cgacagcgat tatatcgaaa tagacgttga ctattccaag taccctatcg
     2881 ctcccgagga tataggactt acagatgttg acagcgtaac agtagccaac ggcgaagtgc
     2941 ttatattcga ggacggcaag ttccagaccc tcagcacagg cgaaacaaag atagatattg
     3001 tttataagga cggctcagta gggcatttta aaatagtgat aaaagaacct gaagccgtga
     3061 agcttgaaag ctcagaagca gaagcaacct caaaagccgc aagtccggtg tcggaagaaa
     3121 agagcggttc atcaatgggc gttattattg gcgtggtctg cgttttgtgc gtagctctta
     3181 ttgccgctga gtatcttatg atagtgaaaa acaaaacagt gggcgacaat aagtcagcca
     3241 acaaggataa tgataaagat atccacgata cagacgaaag cgacgatacc aatttgtccg
     3301 agcctgacga acctgacgaa aatgaagagc aggaagaaac tgataataca gacgacgata
     3361 cagacacaga agaataaaca aaaagcggtg cagagaattg aactctgaca ccgctttgtt
     3421 ttcttattta gctattatta gacaattcct taagctctcg accctcttta agcaggtcat
     3481 gcaacctttg agcgtcatta tttgcaatag catcacgata ctccgttaat cttgcgatta
     3541 tatgctctat ctcgtccacc agtgggccac ggttcataag gaaaagctcc gtccacatat
     3601 cttcgttgag tttggccact ctcgtaaggt ctttgaaaga acctgccgaa aagcctgcct
     3661 gctttaaaag actagggctt tttacatatg aacttgatac aacgtgagca agctgagatg
     3721 tgaacgcaat gacgctgtca tgctcttcaa cgtttgatac aacgaccttt gcaaaaccga
     3781 tattgtatgc aagccttgat atctcctgca ccaccctcat aggcgttctg tctgtaggcg
     3841 tcattataaa gcttgctttc tcaaaaagat tgtccaccga gtatgcaaag cccgaaaatt
     3901 cacgtcctgc catagggtga cagccgagaa agtttacgcc gttttcaatg caaacgccct
     3961 ctaccgctct gactatctcg ccctttactc cacaagtgtc aagaacgata ccgccctttt
     4021 tgaacttgcc tgcattctca gtgatgaatt ttatggtagt ctcagggtga agtcccacga
     4081 taacaacgtc acagcctgca aggtcgtcaa tggccatttc accgtcgata gcctcctgcg
     4141 ccagcgcccc tgctatcgtt cccctgtcaa tatctatacc gaaacagctc tcatgagtgt
     4201 tcttttttat tgccttggca agcgaaccgc ctataagtcc aagtccaact atacaaatct
     4261 tcattgtccg tcatgctccc tttgctttta cgcttttacg taataaagta taccactaaa
     4321 atcacacttt gtcaagagcc atgcacaccc aattattcac cgcaaagctc tgcaatagca
     4381 ttagcgtaca tattcagatt tattttgaaa gtttccatag aaatatgctc gtcagctccg
     4441 tgcatattgt tgttttcctc aggaaattcc gcaccgaacg caacaccgcc ctttgtgttg
     4501 tgaacgtagg ttattccgcc ctctgcgata cattttccct tttcgccctt gaccttttcg
     4561 tagaccctga gcaaagtctg aacgaattca ctgttttcgt ccacataatg gggctccatg
     4621 ccgtcaagct cgtccacctt gaagcccttg ctttcaagtg ccgaagttat gataccgctt
     4681 atctcagcaa gcgtcttgtc aatagggaag cgtatgtcga taccgccatg aagcctgcca
     4741 ccctcagttt taaggatagt cagcgcacag gtcatctcgc ctgatacatc gtctgaaaaa
     4801 ccaagaccgc aggactttcc gttgcattct ccgtgtggga aaagctcagc caacccacca
     4861 aggagagcat tttcattttt gtattctgcc acaaattttg tcaccgcatt gtcgccgttt
     4921 tcaggtcttg acccgtgtga cgattttcct ctgatgacct tctcactgtc gtcggcaaac
     4981 ttcacaatgc acttgtccgg gatagcattt ataacgcttc caccctttat ctcaactatc
     5041 ttatcatctg aaaattcagc agagaatgta agatgcacca tgcccttttc gcagttgagc
     5101 acaggaaaag agccgtcggg ggtgaacacc atttcaggga aaggctgttt gctttcataa
     5161 tattccatgt cctcacagcc accctcttca ttgccgccga aaataacacg gaagttcttt
     5221 tttataggaa tgttaagctc tttgatagcc tttacagccc acagcaccgc aacgctcgga
     5281 cctttgtcgt caattgttcc tctgccgaaa agctttccgt aagctttagt cagcttgaaa
     5341 ggcggatagc tccagccctc gcctgcggga acagtgtcaa gatgtgaaag cacagcaagc
     5401 ttacattctc cctcagggta aaaatcaccg tgaacagcat agccgtcaaa gtcctttgtt
     5461 gtcatgccaa gcttttcaag ataagccttt ccccagtcaa gagctttgcg tgaaccctcg
     5521 ccaaatggtt taacaggggc gtttatgtcg cctacgcttt cgatagaaat aatttcgtct
     5581 aggtcacgaa gtatctcctc catgtgcttg tcaaaatatt ctgcaaattg tttctgatac
     5641 atatttattc ctgcctttca agtgtgcaaa tcattatttc cgagggatta ttcagcctga
     5701 atttaccaag tcccgccgta acagcaagct ttgccaagcc ctgttcagcg tcaagttcgt
     5761 aaatgccctt tgtgtatcgt ggaaagaatt tccgttctgg agaaagtata ccgccaataa
     5821 aaggcaatct tataactccc ccgtgacagt gacccgaaaa cgtcacatct gctccccagt
     5881 ccgcataagc acggaacgcc agcggcgtgt gcgaaagcaa aaagttacat tcgctcctgt
     5941 caggctcgcc taaaagtttt gtaagctcgt cctttgtgat ctgcggcagg tcgtgatgtg
     6001 aaaaatcttc gttgcggtaa aatctttggg gaagctgagt gccgtatata ttgatgtgat
     6061 cctcacctat ttttatctgc tccatgttgt ttctcagaat gtgtactcca cattcggtga
     6121 gtttcaaagc aagggcttcg gcattatcca tgttgtcagt ttcatggtta ccgaaaatat
     6181 aataggtcgg tgcgatatgc tttagccttt tcataagggc aagcttcggt atcatgtccg
     6241 tttcatcacg gctgtaaaga tcacctgcaa agaaaataaa gtcaggctca gcggcaatac
     6301 aggaatttac aagattgttg aaattatccc cgtaacgttt tttgtgcagg tctgcaagaa
     6361 gaagtatctt tttaccaaca aaattctcag ggagctttga tactttcagt tcgtatttgt
     6421 tcacccttag ctgtctgttt gaaataagca tataaccgcc cagaaatgcg gcggcagcaa
     6481 aaagcggcag cttgtatgac attttattac ctcattccta cttgcaaaga tattttttca
     6541 gatcgtccat tgcccatttt gcgtggttgt agccgtaatc gtagagcctt ctcagcttta
     6601 cagggtcgcc ctcagtcctg cctactccga aggtggtctt tggtgtgaaa acaaagactt
     6661 tcccctccgc tttaagctcc ataagctctg cgatacattt gttgtatctc tcggctcttg
     6721 tggcaagggc ttcggcaaac tcagggtgct tgtgataata tttcatggca agctttgtgg
     6781 caggctcttg ggtcttaaca tagccctttg gtcgggtcag cacaacgata tttttgtcac
     6841 agcctgcttt tattgcctgc atatatggta ttgaatctga tataccgccg tccatatatt
     6901 tttctccgtt tatctctatc tcagggaaaa gcagaggaag cgcacaggtt gctctaagca
     6961 aattccagtg ggtgtcatct tttggcacgt ccatgtactc agccttgccg ttttttacgt
     7021 ttgtaacaac tgcacaaaat ctgcccttga agtttctgta ggcttcataa tcaaagggaa
     7081 gctcaacatt aggcacaaca ttgtaggcat aatcaaggtt atagtagcac cggtttttag
     7141 ggtcgataag atgtcttgcg cccatgtatt tctttgttcc cataaactca gttgcaagac
     7201 ggnnnnnnnn nnggagattt ctgcctatct gacctgagca gtaagaaacg ccgaaagctg
     7261 ctcctgccga aacgcctata acataatcgg ctattatttt atcctcaagc agagcgtcca
     7321 ttattccgca ggaatagacc gttctgctgg ctccgccctc taaagtaagt ccaagcatga
     7381 tattacctct gtttctgaaa agaggacggc ttgaccgtcc ccaattttat tttatatctg
     7441 tatcctttaa aattatgcag aaaacgggcg aaggatgttc gcccctacag tttttatctt
     7501 acggcttcag atgttgtatc tgccatgctg ttatccaaag ctgaattgtc aagaactgtt
     7561 tctgtgaact ctctgccgtc gtcggtaaga gcctttttaa gctcgtccat ggcgttcttt
     7621 gttgcgttgt catctgttat ccttacgata acattgtcgc tcataagctt cactgtggaa
     7681 acaagacttt taagatctac cacacgctca tcgattatat aagtgcttcc tgaaaccttg
     7741 atgtcataaa attcctttgg cactgctgtt gttgtggtag taacggaagc ttcattggag
     7801 gaagcactta cgtccttgcc gtcccctgag ccttcgcctt ttccaccgcc tagtccaagt
     7861 ccaagatact gcattataag aataagcgcc gcaatgataa tgacgatcag catgatacca
     7921 agaagctttt tcttcttttt ctttttctgc ttttgtcttt gtttttcaag gtcactgcgg
     7981 tagagctttt caacatcgtt ctgaatactc atttttattg tcctttctat ttggaagtgt
     8041 ttatgtatgt aaagtaaaca ttttcgtatt tgtctgacac agcctgcaca gcgttttcca
     8101 ctattctcat gttacgataa agcacagcgt caccgtcata gaccactgcc gcaagcatta
     8161 cttcaccgtc tgccgcaccg agtccttcaa aaagagatat gagcctgcct gagatatctt
     8221 catcaggggt tacggtcata atatcctcat catttcggga aatatacagc ttatcagtgc
     8281 cgttttcatt tcgcatatca agtgagataa gcataccctg agtgtagctg tcaagcgcct
     8341 gctgattttt tgcggcattg ctgttaatgc tctcagcctt tttgtatgcc tcatctatct
     8401 gcttttgcat atcctcacgg agcttttcaa gctcgtcagc ggacttttgc ttctgctcct
     8461 caagctgttg agtgacagcc tgcacctgag cttttgcgtc ctctttctga ctgtcggcag
     8521 atgcggaaac atcggtcatg acccagaaaa gcattatcat gataacatcg agcagggaag
     8581 tcagctcaag gactatgccc ttatttttcc tgaaaccatt catggctgtt caccgctttg
     8641 ggaatgttca gcgttgtttc tttcaagata gagctgaaca tttttctcgt tatcctctat
     8701 ttttgcagag attatgccgt caagaaattt gaagattata gcaaaaacaa gtccccaaaa
     8761 agtggaggtc aaagcgccat agaaattatc ctgaacgttt gtcatatcgc ccaccatacc
     8821 gaggagggag ataacagtgc cgaggatacc caagagtggg aaaatccctg tgagatttac
     8881 gaacagagaa taaaggcttc ctgtgcggtt gcgcatagaa acgatatctg tttcacgaat
     8941 gcccgaaata tcgctgtcag cctgacggcg ggaagcacct gagtcgggaa cgaagatgtt
     9001 aagatgaagc ttgttgtaga gaacatcagc cgatttttta gtgaaataaa agacgagggc
     9061 ggtcgcgact gcaaccaaga aaattatcat gtcgaagccg aaaaagttca taaaaattac
     9121 ggaaaagagt tttttcatgg ataagacccc ctatagagat aatgatagca cattttgagg
     9181 taaattgcaa gatgtggggg cggattttta gtgaggaggg gaggaatgag aaatgaggaa
     9241 tgaggaatga ggagtgagga gtgaggaatg agcatagcag accaaagtaa attgcaagca
     9301 taaagtaaaa taaagcaccg agcgaagcga gagataaagc gcacgctcaa gcccggcgct
     9361 agagggcttg tggggtctag gggcaaagcc cctagcggag tatgaggcag agcctcattc
     9421 ggcgcaagcc gacaaggtgc agattgtagt atcaaaagca tatagaagtc agatatcaga
     9481 taaaagtgat atcggagaat taaatctgca ccgcctttct agccctaagc gcaacgtaga
     9541 tacgcaaaga gcggagcgat tttgcggagg aataaactat cccaaataac tgtaggggcg
     9601 aacagcgttc gcccgtgttc agaatttgtt ataatttcag aattgtaggg gagcggcgca
     9661 atttctgctt tgcagaaatc cgacgtcccg accccctcgc acgtctttgt acatctgcat
     9721 gactttatgg taaattcaaa cctcgtcact tcgagcgggc tgtcgagggc gccagcccct
     9781 acaagcatga taggatagtt ttttgttctg tcctataatg gttgtaatcg tggacttgtt
     9841 tcttcgacgg gcgaacgctg ttcgccccta cagatttgtt gccgtaattt tgtggttttg
     9901 tacgattttt attcagtttt caagcttcaa acaaggtgca ggaagtgctt ttctgtgcga
     9961 tcagaacctc tcatcgtgtt cacctaactc acgttgacaa aatgtttcct catacactgc
    10021 taccttgcag gatattcaat ttgggataat acccctatac ttatcccgaa aaacaccact
    10081 tttgttgcat aaaaaatgca acaattgcag aaatgttgcc aaattaaaat ataaatcatt
    10141 tgtttgcaca aatttgtgcc actgaatttg tgcatattgg catggtgtga ataaatctgt
    10201 aaatgctgta aaaaataaat gcagattttc aggaggtgat cttatgagaa caatgagaaa
    10261 tctgcttgcg aaaaaagcca tggtgacggt gctggcggtg ggagtttcgc tggcggcggt
    10321 gaatgccttt tggggcggtt cggacagtgc gctctctcag cttgggtcta gcggcagtga
    10381 ggttagggct attcaggaac gccttaaaga acgtggtttg ttcaacgact ctgtgacggg
    10441 atactacggc gaaaagacca aagccgctgt gataaaattt caaaaacagc aggggataag
    10501 tcagactgga acggcaggtc cgcagacgct tcgggcgttg ggcatttcta tcggctctgt
    10561 gccgtcggct acggagcaga acatcaatct tttggcgagg ataatctctg ccgaggccag
    10621 gggcgagcct tatgtgggtc aggtggctgt gggtgcggtg atactcaacc gtatcgagca
    10681 cccgtctttt cctgacacac tttcgggggt aatttaccaa aacggcgctt ttacggcggt
    10741 agtggacgga cagttcaacg agcctgttgc cgcttcagct tacaatgctg cccgtgacgc
    10801 tttgaacggc tgggatccca caaacggctg tgtttactac tacaatcctg ctaagacttc
    10861 aaacgcttat atgcacgcta agcctacggt gacggttatt ggaagccata ggttttgtat
    10921 gtgaaaaaaa gccgccctgc aaaaatgcag agcggttttg tgctatttat gtacgaaaaa
    10981 gtaatcgctg ccgtttaggg atattggtat ggaatagagg atatcgtcgg tgaatttacg
    11041 ggagatgtag cctgctatat cggcgagctt ttcggtatcg gagatgtcta tggcataggc
    11101 gtcaacatac gaggtcttgc ctgttatggc tattcttaca tcatttatat ggaaaacgtc
    11161 ttttacttct tcgaggtatt cattaggtct tttgtagcct aacaaggttt ctgctttgcc
    11221 catgagttta aatctgacaa gggatacatc tacattgcat ttttcaagaa gatctttaag
    11281 ttcttttata ttgtgttcgt agataacgcc tatttcgctt atgccttcct ctgcttctga
    11341 ccaataggta aattcaggct gataacccat attggaaagt tcgctcaaaa ggtttgatac
    11401 tttatacgga tttgttatag gcgtatcgct gacggggagt tcgcctgtcg gctctgggta
    11461 ggcggtgtgg acgtccacaa ggtcgatatt tatgccgtat gcttggaggt aggcttttgt
    11521 gcaggtgacg tcattttcgg tgttcactgt tacatcgagg taggtttcat ggttttcggc
    11581 ggagattatg ccgaagtctg cggctcttga atttagtgcg gaaagtatta tgtcaagatc
    11641 gtctgtgttt tcaaggtcgg tgatgcgttc ggtaaaggtc ggtatctcta tatctgccgc
    11701 ttcatatgtg ccaagctcgt cttcggtgag ctgtttatcg gtctgttcgt cagagtcggt
    11761 ctgtgaactg tcattgtgga tataagttct ttgctcagga actggacctt tactcacaaa
    11821 caggttgact tcttgccctg cttgtatttc ttcccctgcg gcaggctcgg tattcatgac
    11881 agtttcggcg gtgacagtat cgctatatct atcacgtctt acgggcgtgc cgcctgcctt
    11941 tgcgataagc ttttcggctt cggcatatgt cttgttggtg acgtccggca ctgtgatagg
    12001 ctgtgtggtc tggggtggct gtgttgtggt atcggtggag gctgttgagg gggcaggggt
    12061 ggtggtgtcg atatcgggct ttaaaagtcc gctgaatgcg aatgctccca ctgctatggc
    12121 ggcgcaggct gctgctgcgg ctatgccgac ggattttctt ctaagcttta taggctgggg
    12181 ttttgtttct gtgtgtgcgc tgtcggctga gttggtgata aaggctttat tgtctatttt
    12241 ttgcaggata gcatcaagat tttcagactt gctttcgctg tgggactttt tcattatatc
    12301 tgtcattttc attgctttgt tcctcctttt cacagtatgt ttttttcagc ttgtcaaggg
    12361 tgcggtatag tcttgatttt acggtggact gggacaagcc taggcatgat gcggttttct
    12421 tgatagtata gccgtactga taatgcagta cgaaaatgcg gtaggtggta tcgtcggagc
    12481 ttttgagata gctcatcacc tgttcatagt cgaaaaagtc ggcgttttca aggtcggcaa
    12541 gggctttttc atcggggata ttcaggtcgg tgatatcctc aaatgtggct ttgggctgtt
    12601 gtctgtaatg gtctgcaaca taatgcttgg ctatacgcag gacgtaatgc ctgacgtttt
    12661 ttattttccc ctcagtggtg ataagggagc ggtaaaaatc aagatacacg ctttggagtg
    12721 cgtcctctat aagctctctg gtgggaagct ttgaggtgag atagcggttt atataatcgt
    12781 atgtatcatt ataagcctgc acgaatgtat cttttagttc tgttcccatg gtttttcccc
    12841 ctttcttttt ttgctctgtg agctttcacc ctaatagtcg ggaattttgc tgaaaaagtt
    12901 tcagctctgt gaaaagtttt ttgtaaagaa aaaggacggc agaagccgtc ctgataaaaa
    12961 cgctgtgttt agtctacaga ataatatttc tgaagtcttg ttttgaatat ctcacagatg
    13021 atatttgcag ctactcctgc tgtgaataca gatacgaaaa gtatcagaaa tacttttgtt
    13081 gctttgctca tgctatcttc ctcctgtaat tctgtgacgc tgtttttaca gcccttttct
    13141 ttaggtctat tatactataa tcttgctctg attgcaagcg gtttgtgaaa aaatacggct
    13201 ttttataaaa atatttgcaa aaatcaaagc attgtgttat aatatctcta tgtgttatat
    13261 ttttatgtaa tgaggaaatt ttaaatggca gaaacaggca aacaaagcag ttacaaaaag
    13321 ctgttttcaa atactctggt gtttgctata ggctcgttca gctcaaaagt ccttgtgctg
    13381 ttgcttgtgc ctatttacac caactatctt actacggctg agctgggcgt tacggacgtt
    13441 ctcactcaga tagctaactg gataatacct cttgtgacca tgactatcac ggaggctata
    13501 atcagatttg gccttgacaa ggcttatgac aaggtaaagg tctttactct tggcaatctt
    13561 atatgcttct tcggtctggc ggtgttcggc attttgctgc ctattgtgtc ggtgactggc
    13621 attgctgaca agtatattgg cggatacagc ttgatactat atatatatgt attcacatca
    13681 agcatgaagt cgctgtattc aactttcgtg cgtgcaatgg agcaggtaaa gctgtttgct
    13741 tttaacggca tactgaccac gttctttacg ctgttttgga cggctatgtt ttacatggtg
    13801 cttcctgagg gatttctcgg tcatggcacg ggtatcgaga agtatcttct cgccaccatt
    13861 ctttctgacc ttatatcggt ggtatttctc acgttcaagt caaagctttg gagatatata
    13921 aacttcaagg atatcgacag ggagcttttg caggtaatgc ttcaatactc tgtgcctctt
    13981 atccctgctc agcttttgtg gctcataaca aacagctcgg attcatttat gactacccac
    14041 tatcttggct cggactacaa cggcgtgctg tcggcggctt acaagatacc taaccttgtg
    14101 tcaacggtat atcttatgtt cggtcaggct tggaatatgt cggctatcac tgaggacgat
    14161 tctgatgaca gagattcgtt ctatgagaat gtttttgact tcaatcagag ccttttgtat
    14221 attcttgtgg caggctgttt gcttattgtt cagcctgtga ctaacttctg gataggcgca
    14281 gatttccgtg actgcgtaaa atactcgcct atcatcatat atgcgaccat ttattcatgc
    14341 ttcaccactt ttatggggtc tatttatctt gcttcaaagc agacgaagag gtcacttttt
    14401 acgagccttg tttcaggcgt tataaacgtg gggctgaatg ttatactcat accgaggata
    14461 ggactttacg gtccgcctat atccacggtg gcagcttata tgactgtgtt catcataaga
    14521 gccatagact cgagaaagct agtgccgttc aggatagata tgaaaaagat gatagtgaac
    14581 aacatcgtta tccttgtgat gatgttcatt tgtctttttg aaactgacct tatgcaccac
    14641 ctgcctttgt atgctatcgt gacactgctt ttctttgcgg tctttatact caaccgaaag
    14701 gctgtagaga gcatattcta caaggtgctt cctgcaagga tagcttcaca ttttgtgagc
    14761 cttagcactg caaagctttg cgcagttata ggcggcttgg ctgtatttgt actcatagac
    14821 ttgctcacaa ggggcattat tgtgtatgct ttgcttctag caggctttgt ctttgcaata
    14881 tacaaaaaca agaacaggct tatgtatatc tttatggtgc ttatgccgct gagtctagtt
    14941 ttcaggcacg gcatggtatg ggcagcggtg gcatttctga tactggcggt ggctgttatt
    15001 atcaaaaacc gcagggcagc agatgttttc attggcattg cggcacttga tatactgctt
    15061 ggtgcggctg caggactttg gttcggcgtg cttgcggcgg cggtgcagac acttgtatgc
    15121 ggctttatat tccgcagaca gatatgggat aagcttattg aatgggcata taaaaagcgg
    15181 tagagatact gcgttttata gataaagggg ctatgctcct attacaccaa tggaggtttt
    15241 ttatcatggc agaattgaaa tttgagatca cagagcgtat aggcgtactt tctgaaaacg
    15301 ctaagggctg gacaaaggag cttaacaagg tttcctggaa cgagagagag cctaaatatg
    15361 acctgagaga atggaatcca gaccacagca gaatgggcaa gggtatcact cttactgacg
    15421 aagaagtgga aactctcaag gctattctca acggtgagga aatagaggac gatattaacg
    15481 aggctgatat tctttaaaaa attgtaaatg ttcggtaaac agttaaatga taatcaattg
    15541 ttaatcatga gtttaaaaat gtttgctata attttcgaga agaacaatta aacggcggag
    15601 ttgtgcgtta tttcatgcat ggctgccgcc ggaagcttgc acataaaagg cgtttcgtat
    15661 cagtatttaa aggacacgga acgctttgaa cgtacagggc aggtaataat atattcggag
    15721 ggaattatag ttatatggaa cagcatggca ttagcaagct tgatctcaag aggcgcaacc
    15781 gtatgcaggt cctcaagata ctcaagcaga gaggccctac ttcccgtatc gacattgcgg
    15841 gaactttaga gcttacaaga gccgctgtca cgatcataac aaatgaaatg atcgagcagg
    15901 ggataataca ggaaataggc gaatacaaac acgttacaga aaaagctccg agaggcagaa
    15961 aaaagatact tatcgacata aatcaccact acaagttcgt tataggcgta acggtagagg
    16021 aagatatcgt aagcgttggt ctgtcaactc tggcaggtgc agttctcgat aagagaaact
    16081 tggctatcaa cgaaaagacg gactacagca ctatttttga tttcactgag aagtctataa
    16141 acgaggtact gggcgacaac tgccttgaca agaactctat tcttggcatc ggtttcggta
    16201 tattcccgac tatgtattca agacttggta tcagtgttga agatggcgag cctgattatg
    16261 ctaagctaaa ggctgcaata agagagaaaa ctgagcttcc gctggttttt gacaactctg
    16321 taaagggtac ggctatggca aatatcgact tccttaaaac aaaggatata aacagacaca
    16381 acatagcctt tttgcagtat ggcaacacta tgcactttgt tgtgacgaat cttaacgatc
    16441 cgatcgtttc atatgataac agaactaact tcgtggataa gatgataatc aacccttatt
    16501 ctgacaggac ttgtccttgc ggaagaaggg gctgtgttga gagcgaactc actccatcgg
    16561 ctactatgtc aaagcttaag gaagtatttt ctgctgaaaa gacgcctttt ctctatgagg
    16621 cggcaagggg aagctttgag aatgtcaatc aggatatgat aacgatggca tacgagaaag
    16681 acgacaaggc tgtaaaagag atacttaacg gcgagcttac gctgttggcg gtgcttctaa
    16741 acaacttgta tttttctacc aatccacaga agatagtgct tcatcagttc aaattccaga
    16801 atgaaaaggc gttccagaaa ttaaaggacg ctgtgacaga gataggcgga gcgcttgttt
    16861 caagcaagat cgagctttct attattgagg acaaacacag attcctgtca ggctgtgcgc
    16921 ttgcaataag agaattgttc tttaacaggg gcggatttga tacatcttct aataattaag
    16981 agcatcagag cagggctttg ccctgctttt tcttatatgt atagtataaa ctgaacaaca
    17041 tagtgaaaaa tatctaaatt atcgggtaga aaatgtataa aatagagaaa cga
//
LOCUS       DAWEMR010000037        17083 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1406_length_17083_cov_10.398226,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000037 DAWEMR010000000
VERSION     DAWEMR010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17083)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17083)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17083
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1406_length_17083_cov_10.398226"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1957..1966
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10194..10203
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4341 a         3669 c         3875 g         5178 t
ORIGIN      
        1 atatcagcgc agtttatgcc tgtgagcgat gaaataacgc tgtcaatgtg ccttttttca
       61 agtggtatgc gctttgggcg gcactctgtt atggcggtga gatattcgtc acgggtgagc
      121 ttgcctttca cataatcgtt gaaagcgtct gatattttct caccagaatt gttttcgctt
      181 tgctctatga ccgcacaggc acaggcttcg tctaggatat caattgcttt gtcaggaaaa
      241 aatctgtcgg tgacatatct tgcggcaagc ttaactgcat atggtataag gtcaggggag
      301 atgtcaacgc tgtggtgctg ttcatatttg tctataagac cgctgaggat acggcaggtc
      361 tggctttgat caggctcatc tatcttcact ttctgaaagc gtcgctccat agctgagtct
      421 ttttcaatat ttttgcggta ttcctcaaaa gtcgttgcgc ctatgagctg tagaccgccc
      481 cttgcaagct gtggtttcag gatatttgca gcgtctattg caccctctgc cgctcctgca
      541 cccattatgt tgtgaagctc atcaatgaaa agtatcacat ttccagcctt tgcggcttcg
      601 tccatacagc ttttcaatcg ctcctcaaag tccccacgat attttgctcc tgcaagtagg
      661 gcagttatat caagggagaa gatccgcttt gaggacaacg cagacggcac ttctccaagc
      721 atgatctttc tcgcaagccc ctccacaaca gcggtctttc ccacacctgc ctcacctaca
      781 aggcagggat tgttctttgt gcggcggcag agtatctcca ttatacgttc tgtttcagct
      841 tctctgccta tgcaggggtc gaatttttca cactcgcttt tgcgtgtaag ctctctgccg
      901 tagtgttcaa gggtctttag ctttggataa ttcctgtctg aaaaaatatt aggctcgttg
      961 cctgacaatg agcaatcgtt gtacatcttg ttgcagttca cccctaactg ctgcaatatc
     1021 tcaaaggcac agcaatcact gcggcggagt atcatagcaa gaatgtattc cgttcctgcg
     1081 ataccgccac ccatgctctc gcacagcctt tttgcgcctt ttaaaacagc ggcagcgtta
     1141 ggagtgatgt catttttcgt aagacggcat ggcgtgcctc tgcctatgat gtattctatc
     1201 tgctcctcta tcctttgagc cgtgacctcg tggcgcagaa gcactgcaca ggctgtggag
     1261 tcctcttcat aggcggcaga gagcaggata tgctcagtgc cgatataagt atgcccccag
     1321 cttcctgcca gagtgacggc atttttcaca agctctcttg cccttggtgt aaagctgtcg
     1381 ctatcaaaaa actgttcgtt cataaaattc accccttaaa atgtttaaga caaagctttg
     1441 tcttagctac attcttgaca gttttgcgtc ttgttattct cgcattttta tcgcttttat
     1501 attcttatgc acaggacaaa ttctccttgc agattttttt gtaacacatt tttctctgcg
     1561 tcataaactg taatataaag caacggagca aagcttcagc tttataaatc ttaataaaaa
     1621 aggagatttc atttatgaac ggttgtggat ttggcggttg ttggtggatc attatcctta
     1681 ttctcatatt ctgctgtggc tgcggcggtt gcggaaatgg ctgtggcggt tgcggaaacg
     1741 gctgcggtgg ttgtggctgc gacaatggtt gcggcggctg cggatgctga tctgacggca
     1801 aataaaagac ggcggagcat tacgcttcgc cgcttttttt atttatcttc tttttccaaa
     1861 cgcttttcaa ggcgtttcac aatagttttc agtgctttta tgcgggcgta ttttttatcg
     1921 ttgccctcaa gtatcgtcca tggtgctata tctgtgnnnn nnnnnncaac atatttctca
     1981 taggtgtccc acttttcacg gttgcgccaa tcctcatcgg ttatcttcca acgcttttca
     2041 ggcgtgttct cacgctcgtt aaaacggcgg agctgttcgt ctttatcaac attcacccag
     2101 aattttatta tctctgcgcc ccaatcatga agctggcttt caaactcgtt tatctcacga
     2161 taagccatgt tcacacgttc ttcgggggtg agcttttcta taggctctac cattactctg
     2221 ccataccatg tgcggtcaaa gatagtaaag tgaccattct tttcaagacg tgtccagaaa
     2281 cgccatagat agtgccttgc aagctctgac ggttcgggtg cagcgatagg gtgaacttca
     2341 tagccccttg gatcaagggc agccgcaatg cgcttgatat ttccgccttt tcctgctgca
     2401 tcccagcctt catagcatat tataacaggt atcttcttct gatagcagag attttgcagc
     2461 ttgaaaagct tgtcctgata ttttttcagc tttttctcat attccgcttc gtcaagggac
     2521 ttgttcatat ccacttctgc aagagcaggc atttcaatag ttttatattc aggataattg
     2581 tatccgcagg tgtcgaactg tggttcggga atataatgct cgcccttctc cttggcggaa
     2641 actgcagtgc ttactgcctt tgtgaccgct gtcagcactt caagctcagc agaggcacgt
     2701 tcatttgccc aaacgcaagt ccacggagca aatgccgtgt ttgtgctttc aagcattttg
     2761 tcatatctgc ggaaaaactt atcgtatttt tccatgttct ttatatcgtg gctttccaca
     2821 cgccagcttg taacagagga gttctccagc ttttcgatac gctttttctg ctcgtcttcg
     2881 gtaatgtgca ggaaaatttt cacgataaca tatccgtcat ctgtaagctg cctttcaaag
     2941 gtgcagatat ctttaagacg tgtgtccctt gtttccttgt ctatctcacc gtacataaag
     3001 gcattcacag tgtcacgata ccagcttcgg tcaaggataa gaaactcacc ttttttcggc
     3061 aggctttgcc agtaacgcca cagccatggc atacgctttt cctgctcatt tggcgcacga
     3121 taggaaaaca ccttgtaaaa acgtgggtca agagagcgta tgagctttgc tatcatcgag
     3181 ccttttcctg cggcagacca gccgtcaaat gtgattataa caggcactcc tgcggtcttt
     3241 attttgtttt caagcagggt gagctgttct ctcagccctt ttagatattc ggatatctct
     3301 gatttttctg tgtttttctg tatgatcttt tcaagcattg ctatctctcc tctttccact
     3361 aaattgttat tatcacaact taatttattt gatttatgta tattatacac attttatctt
     3421 tatttgtata tagacatttt tcatgaaaat gctatctctt ttttgttaat acggagttaa
     3481 tattattggt ttatactaat atgatataaa atatctcgca aaggacggca gtgatatgtc
     3541 aaaaaaagac gaaaaggaca ttaatatatc aaagctcgac aaaagtctgg agggtcaggg
     3601 tctgttggag gacgctgatg acgcagagta tgagcgcacg ctggctcagc aaaggcgtga
     3661 ggctgaggag cgtgaaaggc aggttttaga gcaggcggaa gctgaaagac gtgagcgtga
     3721 acgtcaggaa gaaaaggaac gtgaacgcag gcttgctcag gagcgtatcg agcttatgaa
     3781 gctcaaaagc ggagttatcg acgaaagcga gtcttccata aaggaagagc atgaccagat
     3841 aagagagctt cacggctttg aaaaggtgca gaatttcttt tatcacaaca aggtgtggat
     3901 aatctttgca atatttatca tagcggtggc gacatttatt tttattgacg ctgcaaggcg
     3961 tgaaaaggct gacctgacag tgcttatgat cgcaaacaac ggcttggaaa cacgtcagga
     4021 ggaactggag gatttctttg aaaaatacac ggacgatctt gacggcaatg gttatgtcca
     4081 tgttgaggtc attatgattc cgctaaattc tcacagcgac gattatcagc agcagaacgt
     4141 gaacagcact aagtttttgg cacagcttca ggggggcgag tctatacttg ttataacaga
     4201 ctcaaacact gacgaggagt tcaagtcaat aatgactcct gagttgccaa aggaatttcc
     4261 gaacaacaag tatgttgatg atatgggcat gagctggaac atggaggtca tggcaaagga
     4321 gcttaacttc gagaatatgc cgaatgatat ccacctttca atgagaacgc cagttaagac
     4381 tttgggcgac tctaaggaaa ctatgcagga gaattatgat aaggcgttca aagttttcaa
     4441 acgcatagtt gacgatatga ctgagaaagc cgtagaggca ggagataagg gtcttacaac
     4501 tgagcctgtc cactatgatg acagcagttt ggaaagctcc gatagcacag aaagctcaga
     4561 aaataagtaa cggatatttg gctgtagggg acggcgttct cgacgtcccg tatgacaaag
     4621 taaagaatag cggacaacca ctttcctgtg gctgtccgct ttgtgtttgt atcagtattg
     4681 ataatcataa tttgtgttgt cctgataata gccgtctgcc tgacttgatt gtgtgtcgga
     4741 agtgttcagg cttgaacagt tttgatcttc ggcatatttg taatcagagg tgacaccaag
     4801 atattcttca aggcaaagac cgctctttgt taccgcctgg gctttttcct tgccgagata
     4861 gcgaatatgc cacggctcat acacatatcc tgttttgtcc tctttgccct cagggtagcg
     4921 gataataaag ccgtactctg cacagtgttc agccagccag tttgcctcag gagtgcctgc
     4981 aaagctgtcc tcggtggtgt taacatcaaa ggtcagaccc gtttgatgtt ctgaatgtcc
     5041 cggacgggaa gatacctcgt ctgcggcttc tgtgccacgc tctgcggcat aggagttata
     5101 aagagattcc tgatcctgat atgaacgata gcttgaattt acaaagagtg ttatgccgtc
     5161 ctgagccgct gcagctgcca tttcgtcaaa ggctttttgt gccactgtgc ttacacccgg
     5221 tgcataatct gatggcaggg agtaggtctt gttcacgata agaatgccgt caacaaaggt
     5281 catgccgttt gactgctcta tcttatgctt tgtttcttct gctttgctgt tgtcggaggt
     5341 tttctttttg gtggtggtct ttgcttccgc ctgctttgtg ctttcggtct gtgatgatga
     5401 atcagcagca ggcttgttgt ctgactttcc actacaggct ttcactatca caaaaattat
     5461 caccaaaaga attgctatgg caatgatacg gtttttcata acacgcctgt gttctcttgc
     5521 gcgcctttcg gcacggcgtt tttccatttc aatgcgcatt ctttcgtatt cttcatcacg
     5581 ataagcgctg tattcgtcag gctggccatt agtgccttgg gataaattct cgttattagt
     5641 ctgattattc ataaatatcc tcttttttgt aattcgtttt ttacgttttg tgttgccttt
     5701 attatatcac aatcgcataa aatattcaag tcggatattg tcggcttatt tcataacata
     5761 aacatagcat tgatatagcg gcatagattc gtattcacca ttatctgggt cattccacat
     5821 atggtctttt ctttcctgaa ctatatcatc atggaaaaag caaacaccaa gatatgtgta
     5881 atcttccaag ccgttgatca gattgtcacc gtaagagaga ccgaagcttg cattattttt
     5941 taaccaagta aaaagctctg tgtatgcctc gtcatagtct gaaacacagc tgttgtttgc
     6001 tgtgaattgt cgtattttgc tgttgttagg cagaccgacc tcagagagat gttcgtatac
     6061 gttgccaatg ccttgacagt agtcgtccat ttgatacagg ctcttttgca gacattcatc
     6121 aagctgtccg cttgcacgga gtggtttcag accatatttc tcacggagag gatttacata
     6181 atcgagcagg tcaagggtca tctgacttgc accatcaggg gagtaatatt caggctcgac
     6241 aggctcatcc tgctgagtat cttctgtgtt ttcctgcggt atatcctgct gtatctcagg
     6301 ctctgtcaca ggttcagtca ctttttctgt agtggtagtg gtggtgactg cggcggcttt
     6361 tgactctgct aatgccgccc cttctgcaat atctgacgca agggcagcct tgtcaggctc
     6421 acgctttttt gtggtagtag tcatgacagt ggtgacggga ggcagagtag ttgtcacagg
     6481 tgtatttgtg gtgacagtgg cagtagatcg aggtgtgctt tttttcacag ccgtggtctt
     6541 atcactattg ttattataag tatacacccc gaaaacagtt gcggatacta atgccacagc
     6601 gattattggt gtggttattt ttgcttttaa aaacactttt ttgggtgtag cgagaactat
     6661 ttcttgcctc ttgtttattt cgctcatgca gccaaagcag aaaatggaat cgtcatgaag
     6721 aattcttccg caattaggac attttttcat attttagttt tctcctgatc ttattaagca
     6781 cattgtacag ctcttccgca gtgctatatc tgtcgtctgg attgatagaa aggcatttct
     6841 ttatgacttt cagaattttc ttgttttcgc acattttgac cgacgggtgt tcacctgtca
     6901 aaagaacgtt cagaagcact gccagagaat agagatctgt tcttgcgtca gattgctgca
     6961 taccgaattg ttcgggtgcg gcaaagccta cagttcccaa agtctgggtg tcagagcgtt
     7021 gattagattt ataaatttta gaaatatcaa aatctataat cttgacatta ttgtcgtcac
     7081 atattataat attatcaggc tttatatcac ggtgaacgat gccgctgttg tgaagagcat
     7141 acaatccctg gcatatggag caggtaatgt tcagggcatc gtgttctgct agtgtttcgc
     7201 cgtttgcata atccgtaaga gtcatgccgt cgcagaattc ttcatagata gtatacgctt
     7261 cagtgctttc ggagatctcg tagatacggc aaagatatgg attatttatc aatgcgagcc
     7321 tttggtatac agaaacgtca gcaggcttat agataatgtg ccgcaccatt tttacacctg
     7381 tgtcactctc taaaatgact ttttcgtgct tgctgttttt aaatagatag tttttgcagt
     7441 cgaacatttt gtcgatctgt tctgtttcaa aatcatataa gcttttttgt gacatatttc
     7501 tcatctccaa tgtaaaaatg taaaaaaata aattattata ggaaggaact ggtgacatga
     7561 aaaaaacaac acagcttatg gaggagctaa atggaagcga tgatatcaac agttttctaa
     7621 gcaacaataa ggattctctt gacagacagg attttgcacg ttatctttgt gagcttgtga
     7681 aaaagaaaaa gatgacaaag atagaggtca taagaatatc ccagctaaat caaatatatg
     7741 gatatcagat atttgcagga gcaagacttc caagcaagga taagataatc gctatcgcac
     7801 ttgctatgaa gcttgatatt gataccgttc agcgacttct gacccttgcg ggtgaagctc
     7861 agctttatcc taaaaacacc cgtgattgca tactcattga cgcaataaac aaaggctatg
     7921 atgtggcaaa gctcaacaaa gtgctttttg aaaatggttc acagtgcctt aaataacgat
     7981 cgtttcataa agcagctccg gttcttcgga gctgcttttt attatcgtaa caaatattat
     8041 atcacatttt gacaaggttt tcaagagttg tcaggacata aaaacacacg ccgcagaaaa
     8101 tttccgcggc gtgtgtttca ctatttaact cttatggaac gagagttgta gttgttgcct
     8161 tatcctttgc agatgtagtt gtttctgcta cagaagaatc ggtctttgct gttgttgtgg
     8221 tcgtagttgt tgcttcgctt gctgcagccg atgaagaatc gtcagttgca ggctttgaag
     8281 ccgcaccagc cttagttgtt gtgtaaccct cggcttcaat taccttcttc ttgaaatctg
     8341 cttctttgtt gtagtaagat gttgagaagt aaggctcttc agtaaatgtt tcacctgttg
     8401 caacgatatt tgagcagtag atcgaagcat agaatgaaac tgcctttttc tccttgtctg
     8461 tctttacagt tatcttgtaa agattgtaga gtgcatttgt gttctcccaa gaatcataat
     8521 tcttcttgtt tgtgaacgca acataagtgt caacgtaatc aaaagactcg atagatgtta
     8581 ccttgccgtc aaagtcagca ggatatggag cgtaatgacc ctttatatcc tttctcatat
     8641 cttcgatctt gtcgtcgaga tatgactgat agctgtccat agcagcctta ccgttgtcag
     8701 ctgttacata tgcagggaat gtttcgtcaa ctgttatttc cttaacataa acagtgtcgc
     8761 cccaatcgtt tgtttcagtt ttgccggaaa gtgtgatacc atttcttgca agtgaggaat
     8821 aagggctgca agttaccttg aatgtgcctc cgatgccaat ataatcatcg catgatactg
     8881 agtaagaaac attgtcctta agagcagcag ggatagactc gctctttact gagtaaggct
     8941 taacaaatgg aacaccgcct gagcattcaa attcaagtcc ctcgaacggg tcgatctcct
     9001 gagttttttc aagaccctca acagtgaaat cctttgttac cttgccgtcc ttaacgtcgg
     9061 ctgtcttgta tgtataatac ttgtcgtcat attcaaacca agtcatgtca ccgctttctt
     9121 ctgcgccata aacgtctatg ttagctgtaa cagtgtactt gtcgccgttt ttcagatctg
     9181 acttgtagtc ataatcatag tagataaatg gatatgctga gtcatatgaa acgtcagcag
     9241 agccgttgcc gtcaaagcct gagaacttga cctcaacgcc cttgaagaag tcgatctcct
     9301 caccattttt aagatccttt acagttacct caaactcagt gttctttagc tttatgttct
     9361 tgtccttgag agaatcctcg ttgtacttta cggtgcattt taccttgtca ccgtttttaa
     9421 gattcttgtt tgctttctta tcaaaagtga tcttcagctt atactcgcca tcgctatttg
     9481 ttttgagaag ggcatttctc atcttcttag cttctgtaaa atcatcagct ttgttaaatg
     9541 cttttgtaag agttttcttg tcaagtgcaa aatacttgct tacctttgat gacttgtcag
     9601 acgagcttgc atagtcggaa agatcatcaa ggtcgccaag gtcgagatct gtctcgtcag
     9661 caaggtcgcc gagagcgctt gtagcgtctg ccattgaata gtagctcttg tcatagcagt
     9721 taagttctgc ctcaacatag ccacagccgt caatgccctt aaattcaaac ttgaaaagat
     9781 cctttgcgtc gatcacctga tatttaagaa tgttgcacag aatgatgatc gcaacgataa
     9841 caacgataag agctgcagcg gcgataagtg ttatcttctt gtgacccttt acccattcgc
     9901 caagcttagc aaaaggattt ggctttgcag ccgcaggagc tgctgcctga gcgtagttgt
     9961 ttaccggctg tgcatactgc tggtcatact gaggagcctg ctgctgtaca ggctgagcct
    10021 gctgaacggg tgcctgagca ggtgctgcct gctgtgtgga aatgcctgct tcggcttcca
    10081 tttcggcgat agacgcaccg cagccgccac agaatttttc ttccggacct attggcgctc
    10141 cgcacttagc acaaaacttg atagtgttca tttcatacct ccattgatac ttcnnnnnnn
    10201 nnnatttgta caataacgca agaaaaaatc atacactttg ataacgtttt agacacaatt
    10261 tttctacatt aaaaaaccga atataaatgg ggatactgag tgtggtatat cttctctgtc
    10321 catgagcgta tgctctcgca ctctgacaaa aattcctcag agcccttttc caatagggct
    10381 gggagctttg ccactgcata ggagatatca tcaagtgtgc aggattgtgc tatatatgat
    10441 attcttacat aatatttatt ccaagcattt tcgagttctt cgagccgtcg gactgtgtcg
    10501 ctgtcctcat ttctgtagct gttttcagcg gcgttcacaa ggcttatgaa cttatcgttt
    10561 tcttttttta ccgcaaatac ggagaacact gacatggcgg ctatgacagt gaggataata
    10621 atgcaggcgg tcaatctttt cattgagctt gctcctttat acttttttct tgtccttgtc
    10681 ttttcttata gcaactatct gccctgaggg gtcggcggtg cagatgtaaa catctttcag
    10741 gggcataccc ttttctccca gctttttcat gagccacttt tcatccttgc cgataagacc
    10801 aagggcggag tatattatcc ttccgttgtc gacaacgagc tgtggagggt cggcctgaga
    10861 tccttttatg cccattccgc cattgttcac aggtcgaaac tgtgctttct gatagaccga
    10921 tatcttgcct gttgtttcca ccaccgcaag ctgtacctcg tttatatcga atatacccat
    10981 gcttcttagg gactccaaaa gatcgtctgc agaaaaacgc aggtctttga gctgtttttg
    11041 atctatgatc ccacgggaca cgactatttt cggagagccg catatgagct ttctaagccc
    11101 tctgaattta agcgagcagt gagagagtat aacgtcaagg cacaccagcg tgaatatagg
    11161 cactatgccc attgacattg gtatagtcat atcctcgata ggcagggtgg cgatgtttga
    11221 aagcagaagc gttataacaa gctccgaggg ctgtaattcg cctatctgcc ttttgcccat
    11281 aaggcgtatg cttatgacaa tgagtatata aagtataacg gctctgaaaa aaactatcag
    11341 cattttgttt tcctccaata tggtcaaagc tattttttgc agctttaatg cagaatattc
    11401 tgataacagc cgcagataat aaatacaaca aaatttcatg taggagaaac aaaatgaata
    11461 cacccgattt cagaaactac aaaaacgctg agatagataa gaccattgac gccgccatga
    11521 cttcccttcg caccataaca ggcaattcaa tggatcttaa cataatgaat gtgaagatat
    11581 gctccgtaag ctgtgcgttg gtatccatag agggaatgat ttcaacgagc gcaatgtcag
    11641 agcttatttt cagacctata atggagcttt ctgcacaaaa gtcaaagggc aatgcagagc
    11701 aggtattcga cttcctgaca aaagaaagcc tgcttgccgc cgagagaaaa acggtcttta
    11761 attatggtga cgtcatacag tttttgtttt cgggctttgc ggtcatattt gtggaggggc
    11821 tttcaaaggc tgttgtttac ggtatccaag gttacgacaa gcggtcggtt tctgagcctg
    11881 caagcgagca gactataatg tgcgcacagg attcttttac ggaaacaata agaactaata
    11941 tttctcttgt gcgccgacga atgaaaacac catcgctgcg gtttgagatg atgcagatag
    12001 gaaagcgttc ttccaccgac gtgtgcatgg tctacatgag cgatcgggca agctcagacg
    12061 ctgtggacag gctgagaaaa cagctcaaag gcataaagct cgatactgtt ctcacaagcg
    12121 gatatatcga accttttatc gacgaaggct tcggttcgtc agtgttttca caaatggctt
    12181 attccgaaag acctgatatg atatgcacaa ggctaaatca gggcaggata tgtgtttttg
    12241 tggacggcac gccattcgtg cttatctgcc catcgctttt tgcggaaaat tttcagacca
    12301 tggacgattt tacggaaaag cctttttacg tcacctttat gcggtggctg aaatatattg
    12361 cgttctttct agcggtggct tttccgggtc tgtatgttgc actggcgagc tttcaccctg
    12421 aggtcttcac attgaagctt cttctgaatc ttgcagtttc agaggagtca acgccttatc
    12481 cgttgacagt ggaggtgctt gtgctaatgc ttctatttga gataatgaaa gaagcaggct
    12541 tgcggcttcc aaagcctgtg ggaagcactg tttctatagt gggcggtctt atcataggcg
    12601 acgctgccgt aaaaagcgga cttgtgtctg cacctctgct tatagtggtg gggataacca
    12661 caacatcatc gttcgttata ccagacgctg tggacaggct gagaaaacag ctcaaaggca
    12721 taaagctcga tactgttctc acaagcggat atatcgaacc ttttatcgac gaaggcttcg
    12781 gttcgtcagt gttttcacaa atggcttatt ccgaaagacc tgatatgata tgcacaaggc
    12841 taaatcaggg caggatatgt gtttttgtgg acggcacgcc attcgtgctt atctgcccat
    12901 cgctttttgc ggaaaatttt cagaccatgg acgattttac ggaaaagcct ttttacgtca
    12961 cctttatgcg gtggctgaaa tatattgcgt tctttctagc ggtggctttt ccgggtctgt
    13021 atgttgcact ggcgagcttt caccctgagg tcttcacatt gaagcttctt ctgaatcttg
    13081 cagtttcaga ggagtcaacg ccttatccgt tgacagtgga ggtgcttgtg ctaatgcttc
    13141 tatttgagat aatgaaagaa gcaggcttgc ggcttccaaa gcctgtggga agcactgttt
    13201 ctatagtggg cggtcttatc ataggcgacg ctgccgtaaa aagcggactt gtgtctgcac
    13261 ctctgcttat agtggtgggg ataaccacaa catcatcgtt cgttataccg agcctttatc
    13321 agcagacggc tatattaagg ctagtgtata tcctggtggg cggcggctcg gggctttatg
    13381 gcattgcact ttgcactgtg cttttgatga taaatataaa ctccatggac gaccttgcag
    13441 tatcttacac cgctcctgtt acgccatttt tctcaaaggg tataaaggac gttatttcaa
    13501 ggcggagctt tcggtcatat gagaaaactc acagcaccat taaagaatac aagcttgacg
    13561 aaaagggggg aagccaatga aaaagcttac gccaatgcag ttttttgcaa tgcttatctg
    13621 tacaagagcc ttttcaatga tgacatttct ccctgaaagc accgcaaacg ctttggagct
    13681 tatgcttgga acagtgctgt ctacagtttt tcagatagtt atgatatgcg gtatggcggc
    13741 tttccacaag cgctttccta atgatgaccc atgcactctt gtggtgagcc gttcaaagtg
    13801 ggcaggcagg ggagtttgta cattgtatct tgcatatttt cttacagtgt cattttatgt
    13861 tatcggcacc ttcacctatt ttatggatta ttatttttct gattatatac cacggctcat
    13921 gatagttctc agcgttgccg cctgcgggat atatgttgca atgtcaggcc ttggggtcat
    13981 aggaaaaacg gggacagtgc tttttgtact ttttctcttt gtgacatcta tccttgttat
    14041 aagctctctt gaagatttca cattcgttga ttttcacctt gcggaaagaa acgttggaaa
    14101 aggcattttt cattctgctg tcagcgagct tgcaagaagc tcttatcttg cagtgatagt
    14161 ttttctttta ccctcaacaa agggcaatgc agcaaaaacg tctgtgtatt ttctgctcac
    14221 aaggcttgcc cttgttgaaa tagtcttagg ttttatcaca atgatacttg gagattacac
    14281 tcttcttgca aaactgccat tcttcgcact tgcggcattt tcacgcactg ccataataga
    14341 acgctatgac gccgctgtta tgggcgtgtg ggtaatgctc tcggtatata agctgggagt
    14401 atatttccat tgttcaggca gatgcctgag atatgttttt cctaagctcg gttctggatc
    14461 gggcgtgata ataacagcgg cgattcctgc ggcggcaacg cttttctggc tcttgcctca
    14521 caagtgggag agcatcgcat acgcagggct cagccctatc ccactcatat ttctcggagg
    14581 cgtaataccg cttctatgcc tgatatttgt gaaaaagggg gcgagatctg atgaaaaagc
    14641 gtaagataca ggtagttgtt gtgcttttta tcgcagcagt gacgctgttg tcttcttctt
    14701 tcggcaggca ggtggatatc gaggagcgtg ttctttgcca cgccatgggc gttgactatg
    14761 aaaacgggga atacaatgtg tcattgcagg tctttaagac ccagagcatg ggttctgaca
    14821 ctcctcttga tgtgagccag tccaatatac agactgtcaa ttgcaaaggc aggagtatcc
    14881 gagacgccat tgagggctgt cagtatcagc taggcaggga gatctttttt ggccatcttc
    14941 aaataatatg ctttaataaa aatatagatc tttcctctcc ggaggagctg ttctcttttg
    15001 ctataaagga caaaaatgtt tttcttggag tggagctttg cttggcggaa aataccgcag
    15061 aggagcttat gaacgttcag ctaacaaggg gaacgatgtc ctcagaaaac ttcacacagg
    15121 tcataaaaat gggtgtgaaa aacggcgtaa cggtggagtg ccgccttatc gatctgcttt
    15181 cctgcataag atcaccgcag tatatcgcta tgccagttat ctctatcaaa caaccgctgg
    15241 gagattttgc ggagagccat gaggaaaagt cagcggaaca gcaggagccg cttttggaaa
    15301 taaaaaagac cgcccttata cgaaaaggta cggtgcttga gagttatctt tcccacgctg
    15361 acgcatcagg ctgtctgtgg ctcacgggaa aggcagagca gtattatgat atgatcgaat
    15421 ataatggaag ccccatggac gttaggataa cctctgacag ccacagcctg aagcttgata
    15481 acgagaacgg caggctcgta tgcagattga agctcactgt tcttgcacac tcttctcttg
    15541 atatccgtga cgagaaaagc tcagaccagg tggcggcact tgtgaaacag cgccttgaag
    15601 aaaacatcac aggggtgtgg aacaaagccc tgtatgagaa cagaacggat atttttgatg
    15661 tgtggcggct tttccgtcac aaatatccgc aaagctatct aaagtatgcc gaccgccttg
    15721 atgaagtgct aggctcggtg gagcttgaaa cggatatcac ctgtcgggtg tcataagaca
    15781 gagcatatcc tcgggaagcg gtgcggtaac agtcactatc ctgccgtttt cggggctttg
    15841 aaaagtcatt ttctcgcagt ggagagcctg acggcttata agagggcttg cctcgccata
    15901 aaggtcatca cccaggagag gatagcccat aaaagccgag tgtactctta tttggtgcgt
    15961 tctgccggtt tcaagaaaga agtccacaag agaaagatct cccagccttt tgcgtacaag
    16021 ataatccgtt gcggaatatt gtccgtcagc cctcacacag cgttttatta tagtttcctg
    16081 ctcccttgca ataggcgcac agatacgccc gccgcagtgg ggcagatctt ttattattcc
    16141 aacataggtc ttttcaaagc ttttttggag cattgcggcg cttcgctgac tttttgccac
    16201 aagcacacag ccgctcgtgt tgcggtcaag gcggttgaca ggtctaaaag cgaggcttgg
    16261 ataaagtgcg gcaaaaagat ttcccagcgt atcgtcacga tgctttattg acgggtggca
    16321 gggcataaaa ggcggctttg agaaaaccac catctcgctg tcctcataga gaatatcagc
    16381 tttcaggtca ggattagggt caagagattc accgtcaccc tcgtttatca tgatgatgtc
    16441 attttcatat acaaggtcaa cagtgcggat aaggacgccc tttcttgtca tgccgttttc
    16501 agtgcgtttg agccttgtta tgagcctttt tgaaaagccc cttgaacgca gtacacattc
    16561 aagggctttt tcattttctt cttttgttat tgtcacagtc atttgctgta tttcctccag
    16621 cgctttatca ttgacggcat tttgtcctgc cagtagcttc ccattatatc ctgcaaaaac
    16681 actatatagc ttagtctgaa atatggatag atgataaaaa caggatatac taacagaagt
    16741 gcggggatat acataacaaa gctcagcagg aacgagatat atctgccatg ctgaccgtcc
    16801 atgagcctta cagaaagatc gcaggcgtca aaaatgttgg tgcgtgggtt tagagtcatt
    16861 atatatggcg caagggcaag ggatatccag taatgcacca taagtccgaa aaacacaaca
    16921 gacagaccca gtgagaacat gaaacaaaga agacctgcgg aggtaagttc gctgagcttg
    16981 catacccagc cccaataata cacgctgtag gatgagatag cgaaaggtac tgcggaaaat
    17041 aatttcagtg aattaattat aatggaagaa aacatggtgt ggg
//
LOCUS       DAWEMR010000038        16338 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1484_length_16338_cov_9.831665, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000038 DAWEMR010000000
VERSION     DAWEMR010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16338)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16338)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16338
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1484_length_16338_cov_9.831665"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7796..7805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4382 a         3836 c         3158 g         4952 t
ORIGIN      
        1 gacaggcggc ataaaaagtt actgccagag cgttgtaagc actatcagaa aatacgacag
       61 tgacgctatt ataatttgcg gtacaggcac atggagtcag gacatagatc aggtgcttgg
      121 caacaagctt tcagacaaaa actgcgttta tgctcttcac ttctatgcaa acacccacac
      181 tgattggctg agaaacagac ttcagaactg ctacaaaaag ggacttcctg ttcttgtatc
      241 tgagttcgga acttgcgacg caagcggaaa cggcggatat aactcaacag aaagcacaaa
      301 atggctcaag cttctcgact cactaaaagt aggctacata aactggtcag cctgtggaaa
      361 gagcgaaacg gcttccgcat tcaatagcgg tacgaacctc aaagctataa agagcggcac
      421 ttcacagctc acagcaagcg gcaagttcat tcgtgattgg tacagaaacc actgataaca
      481 caaaagacct ctcttggtgg gaggtctttt ttttgcacag aaactaacga taagcgggcg
      541 acccaaggtc gcctctacac tataaaaaca aagccgaggg gcatttacct ctcggctatt
      601 gttattttat atcatatcac aaagaacagt gccgttctta taacggctgt aaaaagcata
      661 gcatagaacg atgtgccaag caaaagctga tttgcccaga atatatcgtt aggctcgcat
      721 ggctttagat ttctgcctat aacaggcttt cttacaagct gtccgccgta atactcatca
      781 ctgccaagct ggatacccaa tgcacctgca cacaccgact gagtttgcgc tgaattagga
      841 ctaggggatt ttcttctgtc ttttttgtat acctgagaag cgttcttgct gtcaaggtga
      901 agataagaaa cgttcatttt cataagcagg gcggctattc ttgcaggtat aaagctgaga
      961 acgtcgtcga gctttgcagg aaatttgccg aagtttatgt atgtgtctgt tttatagccc
     1021 actgtattgt cgataatatt cactgttctg tagagtattc cgcccagacc gccgaatatc
     1081 gcagaccaga aaataggtgc gaccgcccaa tctgtggtgt tctctgcaac cgactccacc
     1141 gtacttttta taacagcgtc aatgttcata tcgtcggtgt cacggaaggt cacttttttt
     1201 accttgcgct gtgcagaaga aagattgcct gcccttgcag cacgcatcac tccccctgcc
     1261 gcagttttaa gggactttac agaaagtgct gaccagcaca ttatgccctc gaggaaaatg
     1321 ccaagtgcgg catttagctt ataacccacg aataaaagaa gcaatgccac agcggcaact
     1381 ataagaagca ctatcacagt catcataaga cctgccatgc gctgtgcttc gggggtgttt
     1441 ttatagctgt ttctgaaaga cttatcaagc tttgccacaa gcttttttat aaaatactga
     1501 ggatagacca ctacttcagg gtcgccaagg agcatatcca gcgcaaagcc aagcgcaagc
     1561 gccgccagtg aatttatcaa catatataca tatcctttct tcaggcaata attacttatg
     1621 ataattatat cacaacattt tctgattttc aataccttta tgctttgatt ttaagggata
     1681 aggcacaaaa aagggcggtc agagccgccc gaacaaaagt ctttcaaaaa aatcagaaac
     1741 taaggtgcga acactgtttg cccctacact gaatagatat atccataagc ttttgtgata
     1801 actatattat ttgcatatct ccgaaagctt ttcaagcata agctccattt cctcgtctgt
     1861 gccgatagta attctcagaa agttatctat tctcggcttg ttgaagtatc tcacgaagat
     1921 accattttca cggaggtatg tgaagatctc ctttgcagag agcttttcat gctgtgcaaa
     1981 gagaaaattc gtttcgctgt caaggacgtt gaagcccatt tcacgcattt ttttcgctgt
     2041 tctatccctt gttgccttga ccttttcaac tgtcgccttg aaataatcct cgtcagaaag
     2101 gcttgcaacg gcagtttcta tggctatctg gtcgagagga taggaattgt atgagtcctt
     2161 cacagcctgc atatagctga taagctcggc actggcaaaa gccatgccca ctctcatgcc
     2221 tgcaagggaa cgtgactttg agaaagtctg tgttacaacg agattatcgt actctttcac
     2281 aagctccacg caggaatacc tgccgaaatc cacatatgcc tcgtcgatta taacaatgct
     2341 gtcctgattg ctctcaagaa gttctcttat aaatttctca cccacaccaa ggcttgtcgg
     2401 agcattaggg tttggtataa caacgccacc gttaggctct ttgaaatcct cgatatttat
     2461 tacaaaatca tcgtcaacgc tcttagtctt gtatggtatt ttcagaagct cacaccaaac
     2521 aggatagaaa gagtaagtta tatcaggata aagtataggc ttatcagagt tgaaaaacgc
     2581 tctgaaagct gtggcaagca catcgtctga gccgtttccg cagaaaacat tctcgctttt
     2641 aagcccaaca cgctccgcaa gtgctgccct aagaacagaa gcgtcagcac tagggtattt
     2701 tctaagctcc gctatggttt tgccctccaa tatcgccttt acctttgatg acggcggata
     2761 agggttttca tttgcattga gctttattat cttatcgttt ttaggctgtt cccctgcaac
     2821 gtatggctct atatttataa ggttttttct ccaaagcttt tcgctcattt tacattatcc
     2881 tctctatatt ttatcatagt aaagtattaa tataaaatat tatagcacat accgcttatg
     2941 ttgtcaaata aaagccgcct agctttcaaa gtaagcctgt atagactgca aaacgccgtc
     3001 atagtaatat gcgttcacgc tcatggaatc gcttttccaa tcaagaaact gatctttgcc
     3061 gtttctgtcg ctgacctcag gtgtgccgag aatcgcagcc atctcctcag gagttgaatt
     3121 ttttgttata ccgaatattg agcagtcgct ttcggtggtg aaactcattc ttatcttatg
     3181 gagggtatga tcgctgttga gccagaagtc aaagatgatg atattatcta tagtcgagct
     3241 tgtttttccg tagcaaagag cagaatcgta ttcatcgtca ggggtatcca ccatttctgc
     3301 tcttacatca tcaataagct cagtgcctgt gaaaacgtgg tctatgccct tatagcttac
     3361 gcttatatcc ttaggcagag cagcatctga agtaaggtca agggtcttga ggtcatcata
     3421 tacctttact ctgttctcat cagtgtttac agtgctctcg cccggtgttg ttatgcctgt
     3481 atactcagat gtgaaaacag cctctccgtc tttttcgtgt atttcttcct gcggatagtt
     3541 tttgctgtag tatgtaaaat cggaaaccga gaagtaatca agcactacgt catactcttc
     3601 gtaattacta tatgtttcca ccacgccgag tgaaactgta tagtcaacgt tctcccaata
     3661 cattgtatag catttataat cgtccatgcc gtcatattcg ctgttcatat aaatgcttgt
     3721 agggcagccc attttgtcga ttattttatc aagatcttct ttattgctgt agcagccgta
     3781 gctttcatac tcccagtcag aggtatccac agcctcttct aaaggtatgg taatgttcca
     3841 gctgttttcc ttgagaatgt cagccacact tgcgtcacgg tcaaatgggt ttgcaccggt
     3901 aaggtcgtag tttgtttggc tctcatctga tgaaacgaag gttatctcaa cgtcactgtt
     3961 ttcacctatc attttatcaa tgctcataat atcactagat ctgccaatat cgtcgccgta
     4021 cattttcata ttggcattgc cgccgtctat ggcactaagg tcaacaggca ctgatatttt
     4081 gctaccgaaa atatcctgcg gcaaagtatc tgacggcgta ccgccattaa atccgtctat
     4141 ccaatataga ttgctgtctt tgttatcgtt agcggtatca gacacgtctt catcgccctt
     4201 tgaagctgtg ctgtcatttt gtgaagaagc aacaacaata tcattattat catcgtcatc
     4261 actattatgt gaactaagca ttaccgccgc tgtgatacct ccacccacaa ctgccacggc
     4321 acaaacgctt gctattacct ttgcttttac tgttgaaaac attccttttc ctcccgatat
     4381 agtttttgtt gatgttacgc tatcgggaac tgcattagcg gctgaggctg agcctgtgaa
     4441 aacggtaagt ttcggaagaa cggtgtttga agcctcttta ttaagaagct ttgaaagaat
     4501 aaatacaggc acgacagcat gaagtctgtc gttgttgttt tcctcgtata taagcaccgc
     4561 ctcctttatc ttttttcttg cggcactaag gcgagaagtt actgtacctg tggtgcagtt
     4621 cattacctct gctatctcag gcactgtgag catatcgaaa taatacagta tcaccgaacg
     4681 cctttgctct tctgtgagaa cagtgtcgat aatatccatt attatctttc ttttagcgtc
     4741 attgctcaca tattcctccg ggataagccc ctcgtcaggg atctcctcta ttatctcctc
     4801 tgagttgtcc tcaggggcgg aattgtttct aaagtgcatt ttgcagagat ttatccctat
     4861 cctgtttacc catgctggaa aattctcagg ctgtgcaaga gtgccaagct tttgaagagc
     4921 tgtaagataa gtttcctgag tgatatcctc tgcaagctgt gggtcgccaa gaagcttcag
     4981 gcagctaaaa tacactgttc tgcccgtttc ttcataaagc gcaccgaacg ccgccttgtt
     5041 tccctgtgcc gcctgcaata caagcttttt gatatcatct ttttccatgt ttttgacctg
     5101 ctctttcttt ccgaattgcc gttttctcgt ccgctgtttt atcagtcccg acattagtta
     5161 gatgcaaaac gcagacgatt tgttgagcaa attcaaaatt ttcatatata atttacttat
     5221 ggttaatatt tattattata atattacaga aataattata tcacataaca aaatgcctgt
     5281 caacgaacaa aaaaatatcc cccacaaatg tgagggatag gtttttcaga acatacctgc
     5341 aagatacatt ctgctttcat aaagcttttt tgaatttgta tcgaaggcaa aatgatatct
     5401 gtagagaggt gtaagccctg cggcattttt ctcgcctgca acatatgcaa cgaaaacatc
     5461 agggtcagtg tcgctgttca cacagtatgt ttcgctcatt gacatatcgc cctgtgagta
     5521 aacataggaa agatagtttc ctgaaatgct gttctcgccc tttgagatgc cctgagaagc
     5581 aagcatatcc tgcactgatt gtgctgaata atcatctatg ttgtcatata tctcatcaag
     5641 cttctttgtg cttgtgacat catagcaaag ccttgttggc ataccggtcg gtatccaagc
     5701 aggggtgtaa atatcgtttg acataggcgt ttcctctgcc cataccacat agctgtctgc
     5761 aagagatacc tgtccgtcag ggtttgtgag atttgcaaca tagaccgtta tctcatctcc
     5821 ctgcggctcg acctttggga taagggtcat gtaatagcta tcccaccatt caccgccgaa
     5881 atagttgtca gcacctacac gataattgag catttcgccc tttttatcct catagtgacc
     5941 tccatcgcag tatacaaagc tatactcgtc agcatatttt gcatagaaat cagacgtctt
     6001 tatatatccg cccacctgca tgatgtcacc gttgagctga acaagacctg acgaaagatc
     6061 agcatttttt atctcgtcag acacagtgta aacagccatt tcaagctcgc tgtttgaatt
     6121 tgtaccgctt gcatcatcgt cagtaacatt atcttcatta tcagcatttt catcaagttc
     6181 gtttaggttg tcctctatct cctgctcgtt cacttccccc tgtgcgtctg tctgaacggc
     6241 tgtgtttggc ttatcagcat ctgctgtctt atctctgttt gcaagcacca ccgctgtgat
     6301 acctccgcca actactgcca ctgcgaccac acctgctatt acttttgctt ttactgttga
     6361 aaacatacct tttcctcccg atttacttat tgtagtattt ttatcgggaa caacttccgt
     6421 ggctgacgcc ttgctgaaaa cggataattc aggcagcttc aaatgctgac attcctgcat
     6481 aaatatcttt gacagaacag gtataggcat aacagcatga agcctgtcat tgtttttctt
     6541 ttcgtaaata agcaccgcct cccttatctt ctttcttgca gcgctaagac gggaagtgac
     6601 agtgccaaca gaacattcca ttatctctga tatctcagcc acgctcatca tatcaaaata
     6661 gtaaagtatt attgtctgac gctgttcgtc agtgagagcg atgtcgataa tgttcattat
     6721 tatcctgcgt ttttcttcgc tgtcggcata atcgtcaggg ataagctcgc tgtcgggtgt
     6781 atcgtcgatt atcttttccg actcctcctc agcagtgcgg ggatttttcc tgaaatacat
     6841 tttgcagttg tttatggcaa tgcggtttac ccatgctgag aaattctcag gctctttaag
     6901 gctgtcaagc ttttcaagag ctttcatgaa agtgtcctga gttatatcct ctgcctgctg
     6961 agcatttcca agaagcttca ggcaattaaa atataccgac ctgcccattt cttcgtaaag
     7021 ctcaccgaaa gctgccctgt caccctgtcc tgcctgcaaa acaagacgtc ttatcttttc
     7081 cttttccatt ttttctacca agctttcttt ttattatcga acaaaaaaat atccgttttc
     7141 agcactcggt tattgttccg acatcactta gatgtttgct gaaaacggaa tattgagcaa
     7201 atataaaact taatatattg tttacaaaat attaatcaac atattctctg actctgagat
     7261 gtatacccac gctgtcagca accgcctgag atttttttat ctcctcttcg ccgattatat
     7321 caaccacagt gagcattacc tccggcacga actctttcac tctttttgca aaagagagca
     7381 tttcttcaaa gcagttcacg cccttgaatg agggtcttgt tacctcgtca taagcctttg
     7441 cgtcagaagc gttaaggctt attgagatta tatcaacaag tcccttgaac tcagcaggag
     7501 tttcacgctt gttgataagg tcgctaagac catttgtgtt gagtcttaca ggtgtctttg
     7561 taacagattt tatatacttt gcagtttctt tgagtatttc aagctcacag gtaggctcgc
     7621 catagccgca gaaaataatt tccttgtagc tgtcaaggtc acgctttttg atgttgtcta
     7681 ttacctcttc ggcagtaggc tgatgttcaa gccacagtgg gtcagagcca taagcaccgt
     7741 cgccgttgtg acgaatacag aatgtgcagt tgcatggaca cttgtttgtg atgttnnnnn
     7801 nnnnnaagct tatcgcctat ctcataagaa atattcatag cttttatcat tataattacc
     7861 tcttttttgt aatatatcaa agtatcagag cagacaggcg accaaaggtc gcccctacag
     7921 agaaatcatc gccgcaggcg ataccttaat tcctcattcc tcactcctaa ctcctcacta
     7981 atatctattc tcttatctgt ccttttccag agataagata ctttgtggtg gtcatttctc
     8041 taagacccat aggacctctt gcatgaagct tctgagtgga aattcctatc tcagcaccaa
     8101 gaccgaacat tccgccgtct gtaaatcttg ttgagcagtt tacatatact gccgctgaat
     8161 caacacgctt ttgaaattcc tgtgcggcaa aaaggctttc cgtaacgata cactcagaat
     8221 gtcctgtaga aaatctgtcg atatgttcaa gggcttccgc catgtcatca acgaccttga
     8281 tagagattat atagtcaaga aactctgttg cataatcgtc atcatcagca ggagtaacgt
     8341 tttcagagcc aagtatctgc cttgtaagct cacagcctct tatctcaacg tggtgcttgt
     8401 caagtctttc tttaagcttc gggagaaaat cctctgcaat atccctgtga acaagacagc
     8461 tttcaactgc gttgcataca gacggacgag aggtcttgcc gttatcaaca atgttcacag
     8521 ccatttttat atccgcactt gcgtcaacat aaacgtgaca gtttcctgtg ccggtctcga
     8581 taacaggcac agtggcagac tgtacaacag cctttataag ctgtgcgcct ccccttggga
     8641 taaggacatc aatataatca ttagctttca tcatatcaag agcgatgcct ctgtcagtat
     8701 cctccacaag ctgaatgata tcagggctga agcctgccct ttcgacagcc tttctcataa
     8761 ggtcagcaag cattttgttt gagtttattg cttcctttcc gcccctgagt ataacagcgt
     8821 ttccactttt aaggcaaagt atagcgccgt ctacagtaac gttaggtctg gcttcaaaaa
     8881 taatgcctat aacacccatt ggcactctta ctttctcgat cttcatgccg ttaggtcttg
     8941 ttgagcctga aactacctcg cccacagggt cgtcaagctg tgaaagctcc tcgcaagcct
     9001 ttgccatgcc ctctatacgg ctgtcattga gtgcaagtct gtcaagcatt gcctcagaca
     9061 tttttctcat tctgccgttt tcaaggtcca ccttgtttgc ttcgataata tcgtccttgc
     9121 aaactctcag gatatgtgcg atctcaagaa gtgcgtcatt tttctgtgtt gttgaagcga
     9181 aggcaatatc ctttttgcaa gcttttgctt tctgtcccaa aatatcgatg taactcattt
     9241 ttactatacc tccccgttag ctgtaaaaag tgttcctata ggtttgtttt caaatacctt
     9301 atacaaatgt cttggatctc tgccattcat tattataaga tctattccgc catttttgca
     9361 tatctcagcg gcttttatct ttgtcgccat tccgcctgtg ccaagagaag aacctgcacc
     9421 ccctgcaata gcctttatct cctccgttat ctcaggcaca acagggataa gctcagcgtc
     9481 atcatactta tgagggtctt tgtcaaaaag tccctcgata tcagacatta ttataagcag
     9541 atctgcatta gcaagcaccg caacatttgc cgcaagagaa tcgttctcgc ccatttcaag
     9601 ctccagctca tcaatagaca ccgtatcgtt ctcgttcaca atagggatag aaccaagctc
     9661 tataatgcgg ttaagggcgt tctgcacatt tatgcgtctg tcctcaagaa gaatatactt
     9721 tgtaagcaga agctgagcca cattaaggct gtattcagag aaaagattat cgtaaagata
     9781 cataagctca cactgaccca ccgccgcaca agcctgtctt gtaggagtgt cctttggtct
     9841 ttcctttatg cccagctttg ctctgccaag acccaccgca ccgcttgata caaggattat
     9901 ctcatgtcct gcattttgca gatcggcaag agttttaacg agctgttcca cacggcgtat
     9961 gttcaaaagc cccgtgctgt gagtaagagt agaagttcct acctttacca caactctttt
    10021 cttttttgtc atatctatca tttttatcat gtccttattg ttattttccg ttgacaacat
    10081 tctgcggagt accctctatc cacattttca agttctgcgc aacagtttca agaagccttt
    10141 ctctcgtctg ctttggcgcc cacgctatat gtggggtaac agtgcagttc tttgcatttc
    10201 tgagagggca gttctcactc attggctcgt ttgtgagaac atcaattcct gcccctgcaa
    10261 tgacttcatt ttcaagagcc tgtgcaagag ccttttcgtc cacaagaccg ccccttgcag
    10321 tgttgacgaa aaatgctgac tttttcatca tgccaaggct ttcggcattt atcattttct
    10381 cattatcctt tgtaagagga caatggagcg tcactatatc agaggtcttt agaagttctt
    10441 caaggcttac cgcaggcgtg ccgtcagtta ttttctgggg tgagcgtgta aaggtgatga
    10501 ccttcatgcc aaacgctctt gctatctcag caactttctt tcctatgtcg ccataaccta
    10561 ctataccaat ggtttttcct gcaagctcat aggtagggat accaaaatat gaaaacagct
    10621 tataattcac ccagccgccc tcgtcaactt tctcggcata ttctcttatc ttgctgaaat
    10681 agttgaggat aagtgcaaaa gtatgctgag caactgcgtt tgtagaatat gcaggcacat
    10741 tgcagaccac cgcaccgtgc ctgtctgcgg cggagaggtc aacattatta tatcccgttg
    10801 caaaaagacc gacgtatttc aggtttttgc actttgagaa tacctcctct gttataaggc
    10861 acttgttaca tataacagcg tcagcgtcgc ctatcttttc tgcgacctgt tcatttggtg
    10921 taaaaccata aacaatgctt tcaccaagga ctgttattcc ctccagcgac acatcgtttc
    10981 ttgaaactgt ttcagagtca agtataacta ttttcattct tcatcttcct tttgagcagt
    11041 ttccgacttt tccccggcat tctccaaagc tttctgcttt ttagcaaacc tatgggaaat
    11101 aaaagctgtt gccgcaagaa gcgctacctc tgcaccgaaa agcataccga taacaaagct
    11161 ttttgtggta aactgagtaa ctatagtaag gtcgatagct atcattaatg taagataatg
    11221 ataagagcct ttccttatga actgagttat atataaaagc gtagcgataa cgcagaatat
    11281 catatgcacc ttgaaaggca agggggcata aacgttctgc aaggtcgcag tttccgccgc
    11341 cagtgtttct atcacactat catctcctaa agcattattt tatcatacta agatattata
    11401 ccacagtttt acgtcatttg caatagattt gagcctttat aacggcttta taaaacaaaa
    11461 agacagaagt attttttcaa atacctccgc ctttttcgag aaaaattcgt cgtaatcttt
    11521 aatttatact attttaagat aaattacttg ttagcgaaat atctgtcaag cagacccttg
    11581 aagccgcatc catgtcttgc ttcgtcctta gccatttcat gaacagtgtc atggatagcg
    11641 tcatagccaa gctcctttgc tctcttagca agcttcatct tgccctcaca agcaccattc
    11701 tcagccatat atctcatctc aagatttttc tttgtagaag gtgttactac ctcgcccaga
    11761 agctctgcga acttagcagc gtgctcagcc tcttcgtaag ctgccttctc atagtacata
    11821 cctacctcag gatagccttc tctgtaagca actcttgcca ttgcaagata catacctacc
    11881 tcagagcact cgccgttgaa gttctctcta agacctgcaa caacttcctc atcaagaccc
    11941 tctgcaacgc ctaccttgtg ctcgtcagcc catgtgatag ctgtttcttc tactttattg
    12001 aacttttctg ctggtgcgtg gcagatagga cactcctttg gagcagcctc gccctcataa
    12061 acgtaaccgc atactgaaca tacaaacttt gacataattt ttgtcctcct tattttaata
    12121 tcattttttt acttttccac agaccagcgt tttcgcttta tctgcgttat tctttgcagc
    12181 ttttcccttg ctgtgattaa agtataccac attagtgtac atttgtcaat aggttagttt
    12241 tgtctaacaa tataaaaata ttgcaaaatt ttatggcaat tttttatgca atttgacgta
    12301 gactatacta accaataata cgtcttgaaa aaaacttaaa gctatgctat aatgaagatc
    12361 aatatatgac aaaaaaggag tttttttaaa tgaataagga aaaagcagcg gcagcaaagg
    12421 ctcttttcat ggagggctac aactgttcac aggcggtagt aggcgcatgg gctgaggata
    12481 tcggccttga cagcgaaact gcatacaaga tagcttcggg ctttggcggt ggaataggca
    12541 gaatgagaga ggtctgtgga gcatttacag gtgcagtcat ggtgcttgga ttgaaatttg
    12601 gcaacaccat aggcagtgac agggcggcaa agggcaagga ttatgagcgt gttcagcttt
    12661 ttgcaaagcg tttcaaggaa gagcttggct ctgacactat catctgccgt gagcttcttg
    12721 gtctgagcgg acctagtgac cctgaccctg caaagcgcac aaaagaatac tatcagaagc
    12781 gtccatgtcc tgaaactgta gcaattgcaa gtggtctttt gggagaattt atggagtagt
    12841 ttttttcaga aaaaaacttt gaaactgtgg gcgaacgctg ttttccctac aaaataatgc
    12901 aaaaaagagc agagaaacga gatctctgct ctttttttgt atataaattc tattagattc
    12961 ctgcggaata gtttggagct tcctttgtga tataaatatc gtgtgggtgt gactccttaa
    13021 gacctgcgcc tgttatcctg atgaacttag ccttttcgcc aagctctgca atagtatgac
    13081 aaccgcaata gcccatacct gaacggatac cgccaacgag ctggaagata gtatctgaaa
    13141 ccggaccctt ataagccaca cgaccctcaa cgccctctgg aacgagcttc tttgagtttg
    13201 tctggaaata tctgtccttt gagcctttgt tcattgcagc aagagaaccc atgcctctgt
    13261 ataccttgaa ctgtctgccc tgatagatct cagtttcgcc tggagcttcg tcacagcctg
    13321 cgaggagtga accaagcatt acaacgttcg caccagcggc aagagcctta acgatatcgc
    13381 ctgaatactt gataccgccg tcagcaataa caggaatgcc atactcagca gcagcgcaag
    13441 cagcgtcata aactgcagtg acctgaggaa cacctatacc agcgactaca cgggtagtac
    13501 agatagaacc agggccgata ccgaccttta tagcgtcagc acctgcctcg cagagcgctc
    13561 tggcagcttc ggcagtagcg atattacctg caataaccgg tgtatcaggg aaagcggcct
    13621 taactttttt aagacaattt ataacgttca tagaatggcc gtgagcagag tcaagaacga
    13681 ggacgtctac ctgagcctca acgagagcgg caactctttc aagaatatcg ttagtcatgc
    13741 cgatagtagc accgcaaaga agtctgccct ttttatctct tgcggaattt ggatactgaa
    13801 cagccttttc aatatccttg atagtgataa gacccttaag aacgccgttt gcatcaacaa
    13861 gaggaagctt ctcgatctta tgcttcataa gtatcttctg agcgccttca agatccgtat
    13921 caacaggagc agtaacgagg ttttccttag tcataacgtc accgatcttg atattttcaa
    13981 aatcagtgat aaatctaaga tctctgttag tgaggatacc ctcgagcttg ccttcctcat
    14041 cgactatagg cacgccggag atcttatact tgcccatgag cttatcagct tcagcaacag
    14101 ttttatcggc tgtaagagag aaaggattaa cgataacgcc gttttcagaa cgctttacct
    14161 tatccacctc ttctgcctgc tgagcgatag tcatattctt atggataata ccgataccgc
    14221 cctctcttgc gatagcgata gccatttttg actctgtgac tgtatccata gcagatgtca
    14281 ttataggtgt attgaggact atatccttag taaggtgtgt atggatatcc accatatcag
    14341 gtgtacaatc cgactcgcct gggataagga gaacgtcatc aaaggtaaga ccctctttac
    14401 caaacttatt gtttacatta gtatccagca taaattaacc tcccgcaaac attttgaatt
    14461 cttaatttat tattcttata atcatacaac atttaattaa acttgtcaac atcattattg
    14521 agaacttttt tttaatttag tctttgctaa tcggagttac gttgttttag ccaaggttta
    14581 cgccatggtt tgttatgccg caaaatcgcc gcgctctttg cggattttac tttgtataag
    14641 gatatagtat aagcggtgca gttttgtgct tcgatattag ctttgtatca gattgtaact
    14701 ctgcaattcg tctttttctc tctgcaccgc ttagtaggct tgcgcctatt gaggctctgc
    14761 ctcaaacttc gacagggctt tgccctgtac ccacaatggg tttcacccct tgaccctgac
    14821 caggggcgtg cccctggacc ttttgctcgc ttcgctcggt gggtgctttt actttgtatt
    14881 ggtgcttagc tcagtttggc tttgccattc ctcattcctc actcctcatt cctcattcaa
    14941 tgtctttttc atcactttca ctctcgcaaa caccactgac agcactgttg acacaagcca
    15001 acctatcggc catgacagcc atattcccgt tgacccatat ggtcttgcca acacgaacgc
    15061 aagcactact ctgagtatca agtccgtaaa agttgatatc ataaaatact gcattgcacc
    15121 tttaccacgg agtgctccgt ctgacattat ttttatgccc acaaacacat agaaaggcga
    15181 tattattctc aaaaacgttg tgcctatctc aacggctttt gttccgccgt cgtcaagaaa
    15241 cagatttatc atagtttttg gcatacagaa aaagcagagt ataaaaggca ctgccacgat
    15301 agctgagata acaagtcctg ccttatatcc gctttttatc ctatcgtttc tgcctgcacc
    15361 gtaattctgt gcaacaaagc ttgaaagccc gttagaaagc gttgtaaagc ttgttatagc
    15421 aaaggtattt agctttattg cagaagaata tcctgcgata caagacgagc cgaagctgtt
    15481 tacaagatac tgcacaaaga tatttcccac tgagataaaa ctctgctgta aaatactagg
    15541 tatagaataa agggttatct ttttaagtat ttttcctgaa aacagcggtg gtttatcctc
    15601 tgttttaatt tctgcggttt tcttcaccaa aataagcagt gagaacacgc aagccactcc
    15661 ctgagcgata aaagtagccc atgcaacgcc tgccacaccc catttaaaga acttcacgaa
    15721 cacatagtcg agaacaacgt ttccaactga tgagccgata aggagataca atggtgtttt
    15781 cgagtcgccc attgcgttat aaatacctgt acccacgtta taaagaaaca ggaagataag
    15841 acctgcgata tagatcttca gataaagctc accgtcacgg aagatgttat caggcgtgtt
    15901 tatcattctc ataagaggag tagagaacgc aagtccaaaa actgtcagca ctgtcgcaag
    15961 tatcgtacat gaaattatag tagtagttga cgcagttttt acctttgaga actcttttcc
    16021 accaaagaat tgagatatca ccacacaaca gccaacattg cttcccactg caaacgccat
    16081 aaatatcatt gttataggat aagacgcacc aactgccgca agagcgtcct cgcctgcgaa
    16141 ttttcctgct atgatactgt cgaaaatgga atacatctgc tgaaatatca cgctcacgaa
    16201 cataggcagg gaaaatttcc acagcactgt ccacggtttt cccaccgtca ggtctgtcat
    16261 aaaaacatct ccataatata atagttacca tttcattata tcataaaaaa acaacctttt
    16321 tcaacgtcat ttcgcaca
//
LOCUS       DAWEMR010000039        16335 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1485_length_16335_cov_11.256204,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000039 DAWEMR010000000
VERSION     DAWEMR010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16335)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16335)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16335
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1485_length_16335_cov_11.256204"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5073..5082
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4696 a         3314 c         3760 g         4555 t
ORIGIN      
        1 ggagacagag gattgtatca gatacagata cattttacat ttgaggagga aatataaatg
       61 gcagctacag gaataaaaaa gcttaaaaga agtcatctta tgcatttgtt ggattccact
      121 tttggcggag aaactccgtc atggttcctg atcggcaaaa acattgagga tatgtctatg
      181 gatttgggac cgtctaccgc gactgtcaag aatatccttg atgaaacaca agtaaaagac
      241 aacggttacg aacctagttt gtctgttgaa acctactacg caaatacaga agatgcgatt
      301 tacgaaaaga tcaaatctat tgcgttggat cgtcttgttg gcgacgactg caagagcaaa
      361 tatcttgagg tgcttatcga taagaccgaa ggtccttacg atgcatggat ggaagattgc
      421 atcgttaagc cgcagtcata tggcggaccg cagggcggtg tcaacatacc gtttaatatc
      481 caaccctgcg gcaatcgtat taagggtact gtaacaatcg caaataaagt ggtgacattt
      541 acgccgctgg ctgaaggata atttgagggg cgactaagcc cctcttttct aaggagtgtt
      601 ttttatgtca gaaacaatta agcttagttt tgacgacgga tacaaaaata tcgaattaaa
      661 tgggaatccg gataaaataa tccgtattaa tccaacagat actcagttta ttaaccgaat
      721 ttcaggcttt gatgagaaat atgagaatat acgcagtaga tacggagata tcgatatgaa
      781 ctctatcaat gatctgcaga atcttgatga gaataatccg gactttgaaa agctgaaact
      841 tgccgctgat agtgtggaca aacttgatat ggcggtgaag gatcttataa atgagatctt
      901 tggttatgat atttcattaa tagtattcgg aactgattca tgtctttcgc ctgccggagg
      961 tcaaccaata tttatgaatt ttatgcagtg catcttcgcg tacataaatg aatgttctgt
     1021 ggaggaaaga aagaaatcac aggagaaact taattcctat gcggcacagc gcaataccat
     1081 tgttggtgaa acaaaatgat aggtgcattg cctaaagctt tgacagtcaa cggtaaaata
     1141 tattcaatat atagtgatta tcgcgtagct ttgctgattt tttctatgtg caatgacgat
     1201 acgcttaatg acaaagccaa aacatatgga tgtatacaac tgttgtacaa gcatcacgat
     1261 cagataccta actcggatct ttacgaagcg gcagaacaag ccaaatggtt tcttgacggc
     1321 ggagatatgc caaaatctaa acgtcaacct aaaccgttga taaactggga tcaggatgaa
     1381 gggattattt ttccagctct caataaggta gcaggaaagg aaattcgtga aattgattat
     1441 atgcattggt ggacggtttt aggccttttt aatgagattg gtgagggttt atacagtaat
     1501 gttataaata tacgctataa gcttgcacac aataaaaaac ttagtaaagg agaacaggac
     1561 ttttaccgga acaacaaaga acttattgat attaaggtga agcttacagc agaggaacag
     1621 gatgaacttg attttataaa taatctgtta taaaaaaagt cagcccgttg gactgacttt
     1681 tttattacgc ccaccactga ttaccgcagt taaggcaagt tatacgaact ttttttgctc
     1741 ctttgtttcc ggcaacaaga cctattggac cggcaacagc agttcctatg actgcttttc
     1801 cgactccaaa gccttttttg tttgccgtaa gcgaagtact tccacattta ggacagcaag
     1861 caataccgtt tttcttgttt tcctttatac gctgacgttt tgataacgtt ttttcttcat
     1921 gttgtgtttc gcctgtttgt gatgaagttg atgtggttac ttccatcggt ataaaagtat
     1981 tattgctaag tgcggagaag taaccacata actcagcaat ttttttatta ttgttaaaga
     2041 aaaacattac gttatatgat tttccgtttg ctgttataaa caagcgaccg ttctcggaag
     2101 gagttccggc tgtatatcta atatctgata tgtcggatac ttttatagtt tcgcttttga
     2161 gcccactttt gtatgagatc tccgtttccg aaactattat agttgacaca aaagccgatt
     2221 ttattgtggc attcataggt atagttgaaa tcgagttgac ttcttcaatt tcggttttat
     2281 tgtgatgcag aatttcagtt ccacatttca tgcagaaatc tgattcatcg gatatttctg
     2341 cgccgcattt aaagcaaaac ataacatttc ctcccattat gtattttttt cattatacag
     2401 cgtatggagg attttgtcaa gaaaggaggc tgatataatt ggcaattgac ggcagactta
     2461 atttcgatac aaaaatagat acaaaaggat tttccaaagg tataaacagt ttaggtaacc
     2521 agcttaataa tctccgaaat atagttttaa aaatgggtgc agcacttggt actgtgttca
     2581 gtggaaaaga agctcttgaa gctgctgcag atataaatgc tgcaaattct caaatgcaac
     2641 agtcttttgg aactttaaaa tctgctgcag ataatgctat gaaaagtgta gctgataaca
     2701 gtagcattct tcagacaaga cttcaaaatg taggcacatc tatttatgct tttgctaaaa
     2761 ctacgggtat ggattcagtt agtgctctaa aaatgatgga agaagcgttg caggtaacag
     2821 cagacagtgc ggcatattac gatcgaagcc ttgaagatac tgcagaaagc ctaaaatcgt
     2881 tcttgaaagg caactttgaa aacgatgctg ctttgggttt gagttgtaca gaaactacgc
     2941 gaaacacagc ggctaataaa ctctatggaa aatcatttat ggaattatcc gaagctcaga
     3001 agcaacttac actattgcaa atggtcaagg atgcaaatgc tctttctgga gcggagggac
     3061 aagccgcgcg agaagcagac ggctgggaaa atgtcatcgg taatctgaaa gagtcttgga
     3121 aacagttgct tgctgtaata ggacagcctg ttctttctgg tgctgtaaca gttgtaaaaa
     3181 acataacagc ggaattgcaa agtttgacag ctgttgctaa ttcagcagtt aaagcacttt
     3241 ctgaggtgtt tggaattaaa ctgatgaata caacagatgg agttgctgaa agttcttcgc
     3301 aggcggcgga aaattattcc gatatggcaa catcggctga agctactgtc gaggctcaag
     3361 aaaatgcact tgcaagcttt gatcagataa ataagctggc ggacaacagt tcctcatctg
     3421 atacaaatgc atcgccagtg gtcggtactc taagcggcaa tacgatctcc actactgtag
     3481 atgttgatac atctgatgcc gataaaaagc ttaaagattt tttttattgg gtaaaatcat
     3541 cttttaatac tatttttacg ccatttaaac aagcttggga taaaaatgga gtcaaggtaa
     3601 cagatagtat gagatttgct ttcgagggtg tatggagtgt tatcaaaagc ataggcggat
     3661 cattcaccga tgtttggagt aacggaacgg gcgaacaaat ttctgaacat ttactcggta
     3721 tatggacaaa cattaataat acaatcggat atgtgtcacg caatttttcc tccgcttggt
     3781 ctgatagcag tggtacaaaa attattcagg acattcttga tatttttaat gatatactcg
     3841 acacaattga aaacataacg gcagacactg ttgaatgggc gcagaacatt gacttctcac
     3901 cgctcattac atcatttgaa aatgtaacat ccgcattaaa gcctttaact gccgacatat
     3961 ttgacggtat cgaatggttc tgggataata ttttgctccc tatggcatca tggactataa
     4021 gtactttgat accaacattt cttaatttgc tggcggcagc tataaaagtt cttgattcag
     4081 caatttcagc gttaaaacct atgggtaaat ggctgtggga taaatttttg aagcctattg
     4141 caacatggac cggaggtatt atagtaggcg cgttgaaagg tattacatca gccttaaatg
     4201 gggttagtga ctggataaag aatcatcaga ctgctgtcga aaattttgct gttgtagttg
     4261 ggactttggg atcggcattt gcaatatccg gaataattca aggcgtagta agtgcatttg
     4321 ccgcattggc ggcaggaaca agtgtattga caccgttaat tactgcactt ggtgtagcag
     4381 ttaatttttt gacgagtcca atcacacttg tatgtctagg aatcggtgcg cttatcgcta
     4441 tcggcgtatt gctgtacaaa aattgggaaa cagtaaaaca gttttttatt gatttgtggg
     4501 acagctttaa aatgaccata cagcaatttg tagactgggt aacagaggtc tggacatcaa
     4561 ttaaagactt tttcgccgga atatggcaag gcataaaaga tgtatttgcc gtcgtggcag
     4621 aatggtttac gggaattttc caagcagctt gggacggtat tttgtctgtc tggaatgccg
     4681 ttataggttg gttctcaaat ctgtggacag gaatcaaaga cattttttct gcagtaggaa
     4741 gttggtttgg agatatattt acaactgcgt ggacaaatat aaaatcggcg ttttcggcta
     4801 cagcacaatt tttcagggat ttgtggactg caataaaatc accgtttatt aaggtagctg
     4861 attggtttaa agatatattt tcaaaggctt ggcaagcagt taaggacgta ttttcgactg
     4921 gtggcaaaat ttttgacggt atcaaagagg gtataacagg agtattcaca acggttgtaa
     4981 acggcataat tggcggaata aataaagtta tttctactcc actggatttt cttaatggca
     5041 tacttaatga tattcgtgat attgaaatag cannnnnnnn nncatttgat gagttttggg
     5101 actatgaccc tataccagtt cctcagattc caatgctcgc caccggcgcg gtaattccgc
     5161 cgaactccga gtttcttgcg gttctcggcg accagaaacg cggcacaaac atcgaagctc
     5221 cgctggatac gatcaagcag gctttgtttg aggcgcttgc tgtttacggc ggagctgtag
     5281 gcaatcagaa aataagcgta acgataccaa tcgaagtaaa gggcagagtg ctgtcacaga
     5341 tcgttattga cgatataaat gattttatca agcgcaacgg caaatcgccg ataaaagtat
     5401 aggagggata ctatgaaatc aaatggattg aaatttgaag aagaaacggt agccactcct
     5461 gctgaaatta ctttttgtaa caataaaatc tggtcgggca atgcagggcg caccgctaat
     5521 tgtcttatgg tcggagacat cagggctata aagaaaacgg tcactattaa atggtatcat
     5581 ctcacaggcg agcagactgc acagataaat aagtatatct caaatgttga tagcccattt
     5641 tttaacgcca cacttttgga cgagactttt aatgaaatca aaataagggt ctatgcagga
     5701 gatccaagct atgaaatatt cggctgggac gaaaagcggc agttttgcaa aggcgttgct
     5761 gttgacctta tcatgcagta ggaggcggat atatgtatac aacaagtaca accatctcct
     5821 cacgcatcga aagctactgc cgcacatggc gtatgtggct tgaaaacgac gagagcgtaa
     5881 taatggggga caacataatg tccgctacca gtgacgtgca gtcaacgagc ctcagtgacg
     5941 acatagagct tggtgcagtg tgctcacagt cttgggcatt acagataaac gatgctgaaa
     6001 cacgtttcct cggcaaagag tatgacctgt ccctgtacct tgcagacctc acaggcgtga
     6061 ccacctactc caccctagaa gcctacacct acgcagaact ttcaaagctg acagtggagc
     6121 agataagcaa gcttggagag gtgcttgacg gagagagaat acctctgggg cggttcacct
     6181 gcgtgaaatc gaaaaagtcg ggcggaaata ctgaggtcac ttttgcggat aggctttact
     6241 tctccgacaa gacctatgtg ccaaaggtca agctacctgc gtggtcaaag gctgtcgagg
     6301 acgacatatg caagcagctt ggactgcaaa acggcaacga ctacaccatc cctgcaaagc
     6361 tccgtgtaaa gggcggtgca aggctttacg gcaagggtca cataaggctg aaaactgcaa
     6421 acttcgactt caaaataagc tctataccca aagacaccac aatgcggcag atgctcagtt
     6481 acatagcttc ggcacaaggc gagttcggtt ttgttgaccg atacggcaga tacgtccgca
     6541 aatggtacgg ctcgagcgtg aagatactgg acaacaacac tatcgacctg ccaacactgg
     6601 gagaacgtcc gaatatcctc gcaggcattg tctgcaaggt cagcgacagc gaaactctgc
     6661 ggctgggcaa taccacaggc tcggcagggc gtgtgctgga gtttgaaaat ccatatatga
     6721 caatgtcgct gctgcggtca ttgtggcata ggataggcgg cttttcgtgg tatacaacgg
     6781 agctttttca ccgccttggc gacccacgat ttgacgtcgg ggacgttgtg acatacgtca
     6841 gcgacagcgg tgaaagctac gatataccaa taactaacat aggattcaat tttgacggcg
     6901 gactttcagc cgatatttct gcggtgggtc tgtcggtgga agaacagctt taggaggcga
     6961 gataatggac gaaaatgaga taacaactgt ggctgatacg caggcggaga atactgccga
     7021 tacagcggac acaggtcaga caacgcccac caccgaggag tttatccagc agctcacggc
     7081 gagggtggca gctcttgaag aaatagtggg cgaggaggag tatgagctgc ggtactcggg
     7141 cgaacagacg gacgagcttt tagacggcgg tacagcggtg tttcgtgcaa agacagcggc
     7201 gcagatagta agccttgtga acaggctcta cccactgtat atgcggtggg ggtctttcac
     7261 ggtgaatatg aaggtcaacg ccgacaacgg ttcccagtgg tcatacaata cacgcacagg
     7321 aatgataccc tcgggggtca ctaagcctgc ggtgtttatg gtgtgcgact ggggcaaaaa
     7381 gcacttcaag tcgcagagtt ttcaatacaa agtcgcaagc aacggcaggg acatcgactg
     7441 ggaggcatac cttgagcaca cctctgacca gggcggcaca tacgctttca aggtatacta
     7501 tctcatagtt ggcaaaaatg cggaaggggg aagtgtagtt ggctagtttc acggaaaatc
     7561 tcggacttaa aaagcccgac aggacggaca ggttcagcat cgaggacttc aacggcaata
     7621 tggatattat cgacactata cccgatatgg cgagcggaca gagccttgtg ggtgtgtcag
     7681 tgggagaagc gtacggaaat ataggtataa taggcatagc ggaggcggtc gaagatgaaa
     7741 atatatgagg gaacagacgg actaagagga ttagtcaaga agcttatcga ggtctatgac
     7801 tttaagaaag ttgtgttcga gggcgataat gcgagtattg atacacaaaa tgccaccttt
     7861 cagctttggg taacagatga gctgttttta agagggcaat tttctgatac aaatagtaac
     7921 tttggctggt gtgacttgag gacacaagca ttaacttgtc cttgtgtaag tactgcacct
     7981 cgtactggag atcctagacg ttgggtcatt tacaaacaaa atgacctagt tgctatgggt
     8041 atagacagta ataccactag tagacctggt ataaatataa taattggtga agtaactaac
     8101 tatgaaacag gagaaaccga aataggaatg gccacaagtt gtgctgataa taatattcgc
     8161 ttatatacag tattcactga tggggctact attaaatcta ctccttatag atatttttgt
     8221 cagcagaaat cggtgacttc acttgctcct gtagtttcta ctgatttaaa caaaggtttt
     8281 acaaatgtgt atcatatact ttctcatata cagggtatat cagatagcta taataacagt
     8341 gactatgctg tacctacgca aactatactg ctcaataata agaaatatct gttaagcaga
     8401 tttgcttttg agataaagga gtgagcaaga tatgaaacag aaatttgcaa agcttataga
     8461 cgtcaagtct attgttacga tactgctgac ggcagtgttc tgcgtgttgg cacttcgccg
     8521 cacgatttca gcagagcagt tcatcacggt gtttactgtg gtgatatcgt tctatttcgg
     8581 cacgcagtat cagaaaaact ataaaaataa caaggaggat aattatcatg gcagcgacaa
     8641 ttaaaggcat tgatgtttct atgtatcaga caaacgtaga tttcgcaaag gtcaaagcgg
     8701 tgggctacag ttttgttatt atcagatgca ataactggga taacacgaag aactgtgtag
     8761 taaaagaccc gctttttgaa acgcattaca aaaatgcaaa ggcagctggg cttgacgtcg
     8821 gcgcatatta ctatacatgg cagacaacgg tatccggtgc gaaacaggac gcagttcttt
     8881 gtctcgatta catcaagggc aaaacttttg aatacccgat ttactttgat ctggagtggc
     8941 agaaagcttt tgcacgcggt aaaacggtat gctccgacat ggtaaaaact ttttgcactg
     9001 cgctggagga agcaggctac ttcgcaggtc tgtatatcag ccgcagtccg ctccagactt
     9061 acataacaaa tgatgtcgca agacgctatg cactgtggat tgcagaatac aacagcaagt
     9121 gcaactacgg cggcacatac ggtatgtggc agtacagctc aacgggcaag gtcagcggtg
     9181 tttccgtgcc ggtagacatg gattactgct atgtggatta cccgtctgtg ataaaggcta
     9241 aggggcttaa cgggtttaag tctactaaca caagcacgtc taaggtactt gacagttcgg
     9301 gctttaagaa aggtgataaa tccgatggag ttcttgcact gaaacagctc cttatgctgg
     9361 cagggtacaa acttgacaac aacggcacgt tcggagacgg taccctaaag gcggtcaatg
     9421 ctctgttgaa aaagtggggc tatactcaga acggtattgc ggggactaaa tttattaaaa
     9481 agctgtctgc aacgataaag taaaggagta gcttatggat acaaaagaaa catcatacag
     9541 ccaaatggtg acagtcacta ggcttaatta caggagcgat tgcaacttca ccgccggaac
     9601 gatcgttggc gttctcgaag ataatactcc ggtaaaagtc gctgatgatt tttatgaatt
     9661 tcatcacggt cactactgga gaaaaatcaa gcttggtcgc aagcattatt atgttgttgc
     9721 tgattggctt aaaaagattt aaaagtaaca gctccgggca atccgctcgg agctgtatac
     9781 tatattaaaa ggaggtcata tttatgaaaa gtccaatacc atggattggt ggaaagagcc
     9841 agcttaaaag taagatcatc aagtctttcc cgcctacaga aagctacaac agatttatcg
     9901 atgtattcgg cggaggcggg tctatacttt tcgcaaaaga caaacacgct gatctagaga
     9961 tctataatga cgccaacagt gatctggtca actttttcag atgcttaaaa tatcattctg
    10021 atgagcttaa aaaggagata aaatactatt taaacagccg tgaaatgttc cttgactgcc
    10081 gcgagcgcat atctgtaacc ggatttacag acattcagcg ggctgctatg ttctatgtgc
    10141 ttgtcaagac aggcttcgga gcaagtctga gaacgttcgg ctgcaacaaa aagcggctta
    10201 acacagataa tttcgcagat atcgaggcaa gattggatgg agtagtgatc gaaaacaaag
    10261 attttgagga tcttatcaag gtatacgacc gtgagaaagc tttattatac tgcgaccctc
    10321 cataccacaa gacagagcgg cattacgctg ttaaatttac cgaggatgac catgagcggc
    10381 tctgcagggt tcttcaccag atcaagggca gatttgtact gtcgtacaac gatgacaagt
    10441 atgtgagaga cctgtataaa gactacaata ttcaggcggt cacccgcaat aacagtcttt
    10501 catcaggtga tttcaaagag gtaataatca caaatttcta gtattttttt tagagaataa
    10561 ataacggatt tcgttattta tgttgtaaaa aacataccgg aggtaatcat gagagtaaaa
    10621 ttaagggctt tgcttaattc caggggcatt actcaggctg aacttgcgca ggcgacaggc
    10681 atcagaccgt ccacgatctc acagctttgc aataacatcg ctgtcggttt caaattttca
    10741 catcttgagc tgatttgcag atttttaaaa tgcgatttaa atgacatttt agagctgtaa
    10801 aaaatacgat tcaaaaattc ccgataatgc aaaaaaagtg atgagtgttt cgttttgttg
    10861 aaacatttca tcgctttttt gcgttttgct tgtcagtttt ttggattttg cttgtcaaac
    10921 atcaattgtt tgtgcgatgt ttattcaatg tgaaaagcaa cataaatagt gacgtgcgag
    10981 aaaggcgggc tgtcgagggc gccagcccct acattgtttt gacacacaaa aaaaggacgg
    11041 cttttcttcg ccgtcctttt gatttctaat ttagtcctcg tcagcaagtg caaaatctat
    11101 cttgccggag tttacatttg cgtttatcac ctttactctg atagggtcgc ccacacgata
    11161 ttccattccg tttgtaaggt ttttcaggct caccatatcg tcgcagacat actcgccgtc
    11221 aggcatattg tccgtgtgaa gaagtccctc gcaggtgtca ggaagtgcga taaaaagacc
    11281 aaactcaaca gctgaaacca ctgttccctg aaactcctca ccgatatgag cgttcatata
    11341 ctcagcctta tagcagtcct cacagcttcg ctccacctgc attgcggtaa gctccgtctt
    11401 tgtggactgg tcggcggagg cgtaaacgaa cttgttgaac tttgtggcgc actcctctgc
    11461 actgctgcca ctgagaaaag ctgacattat cctatggata gtcaggtcag ggtaacgtct
    11521 gataggtgaa gtgaagtggg cgtaatcctc cagcgcaagt ccgaagtgac ccacaggctc
    11581 ggtggaatat cttgcctttg acatactcct cagaacgata ttgttcatga taaggtcgat
    11641 atcagtaccc tttgcggctt ttagtatctc cgacatatcc ccagggcaag ccttttcgcc
    11701 cagcttatac tggacgttga gcttgacaag cgcttctctc agggattcaa gcttctcgtc
    11761 tgacggctct tcgtggatac gataaacgaa cggcaggtta ttatccatgc caaatcttgc
    11821 ggcacactcg tttgcaacaa gcatgaagtc ctctattatc tcctcgcttc ttccacgggt
    11881 acgcctctca acgcccacgc agatatcctc gtcattgatg ataagcttgc tctcagaggt
    11941 ttcaagctca ggacagccac ggtttttctt gtttttgtac aaaatatccg caagctcctt
    12001 tataatagga agctgacctg aaacatcggc atatttttct ttaagttcag catcgtcact
    12061 gcctgccaaa agtgaattta tctcggaata aacgcccttt acacgggagc ggataacagt
    12121 ttttgcaaac ttatagtcgg tgatattgcc ctgcttgtca agttcacaaa gacatgagaa
    12181 cgcaagtctg tcctcctgcg gattaaggga gcagatacca tttgaaagct ctttcgggag
    12241 cattggtata actcggtttg cgtaatagac gcttgtgcca cgcttgaaag cttcgttatc
    12301 cagttgtgac tttggctgaa cataatggga aacgtcggca atgtgaacgc caaggagata
    12361 acctgttttt gttcgttcaa cagaaattgc gtcgtcgata tcctttgtat ctgcgccgtc
    12421 aatggtgaat atcggcttgt cacgaaggtc aaggcggttg ggtatctcct cttttatcct
    12481 tgaataatcg gacacttccc tagcttcata gatgacctcg cttgggaaaa caggggtcag
    12541 accattgacc tcaaggacgc tcattgcaca ggcggaggct ttcatggagc ttccatagct
    12601 tgaaactatc tcgcagacgt gttcagagtg cctgtttcca cgctttgtga cctgcgccac
    12661 caccttatcg ccctcgtgaa gctcaagacg cattgggttc tcaaagatca ttgcatattt
    12721 cgacagcata tcaggaacga ttttcagcac gccgaactcg cttacaagct ctcctgtgaa
    12781 cttagcaaaa ttctcctcta cgatgctcat gacctcgccc tcggtgcaag ccccctcgcc
    12841 cttgaaggtt ctcacaagga cgatatcgtg tggcattgcg cctttgaggt acttgcccac
    12901 aacaaaaatt tcctcgtcag tgtcaagatt tttcacaaag ccgtaggtct tattgagcct
    12961 taccacctcg cacttgacga acttcttagg gtcaacgagc cttatgccga atttatcttc
    13021 cattatttcg ccgtttttct tcattttgtc aacgactttt gtgaattttt caaactcaaa
    13081 gcccttccct ctgcatgatc tcagcagttc tttaaaagtc agtggctttt tgcctgcttt
    13141 tttcaatttt gagtatatta tttttctgta atctaacttc ataaatttcc tttcttttta
    13201 ctgttacttt actttccaaa agaaaaagcc ctgcacggag cagggcgggc aattatttct
    13261 tgatctgata aatgatattt acagcaagag ttatcacgaa tatcagtatc gcacttatcc
    13321 ttgtcagtct gttaagctta gcgtccttgg tgtttgaacc gttctttcca tagaaagaat
    13381 cgttatatcc accgccgata gcagatgtaa gaccctgctc tttggtgtcc tgcgcaagaa
    13441 ctacaagtat gataacgagg ctggctacga tcagcagtgc gcctgcaacg atctcaatta
    13501 cgctcatttt tgttcctcca aaacataatt atacatttta atatattata gcacacatct
    13561 tctcagattg caaggggttt tcagaatttt ttcaattctt tttcttttta tcggtgcttt
    13621 cagcctttga ggtggtatct ttctgcttct ccacctgctg gataactgtg acgtttgaca
    13681 cgtcaagacg ttttttcaga agctctgtga ccgttttgag ctgttcatcg gtaagagcct
    13741 tgttctcata tagcacaact gttatgccat taactgttgt gggcgagcct actgaatggc
    13801 tgctcatgtg accgcctgcc gcacctgtta tgtttggata aacgatagca agctcctcgg
    13861 cgatatcgct catgtctata tcatagctct gatagctgtc aagctgtgcc ttcagatttt
    13921 cattctcggc tttaaggtcg tcgttttctt tctgcgcaag cacctgtgcc accttgttct
    13981 gtgtttcctc atcgtcggca ttcttttcaa gcatcttttc gacctcgtca gcggtaacac
    14041 cgctttccac ctcggtctgt gttatcacaa gcttataatc tcccaatccg aaagctttca
    14101 gctcgctttg aagtgccttt gtatcggcag tgcttatctt cttgccaaga agtgccactt
    14161 ctatcatacg tttatcggta tctatagttt tctgcactat ctgagtgccg tcaaagtcaa
    14221 aattctcggt gataaacttc tctgcactgc tgttatccat tgtctttttc acaatatcat
    14281 agccaagata aatactaggc agcatggtaa taactgtgat aaaagcgata tttctgtgta
    14341 tctttttcaa agccttcggg gaaatatcgt tgccgtgagg cagccttaaa aacttcagca
    14401 caacgatagc cgcaaggcag atgaaaaagc tgtttatgaa aaagagatac aatgctccgc
    14461 cgaaatagtc aaggcggtgt cttgccaagc cgtagcctgc ggtacaaagg ggcggcatga
    14521 gtgctgttgc gatagccacg cctgggataa cgttgctttt ctccttacgg gtctgaccta
    14581 ttatgccggc aagtccgccg aaaagggcaa taagaacgtc ccacgctgta ggtgtggttc
    14641 gggcgagaag ctcggaggag gctgtggtga taggtgagat agtaaagtaa atagtcgagg
    14701 tgataaggca aatgaccacc tgtatgccaa gtcttattgc cgcacccttt gaaagagtca
    14761 ggtcattggt ggcgattccg tagcctatgg ctgttatacc gcccataaga ggtgagatga
    14821 gcatagcacc gattataacg gcggtggagt tcatattcag acctatggaa gctataagta
    14881 tggcaagcat gaggatacac atattagtac cctgaagttt tgcgccagat attatcgtat
    14941 tatgtatctc atcagggtca gccatatccc agcgcagact gaacatattt ctaagccctt
    15001 gtataaatct gccttcctgt aatttcaaat ctcagacctc ccctgcttat tttactgcac
    15061 cccatgcgat gttctgcaaa cgccttgcag aatcggtagt gcctgcgcct gtctgctgga
    15121 taaaatatag tatgcagagc tgttcttttg ggtcaatgct gaaataacag cccatccagc
    15181 cgtcccagcc gaaagagcct tctgactgga taagtcctgc ggtggcaggg tcgtcaagga
    15241 tacgcataaa gttgccgtag ccgtgaccct ttgtgctgtc ccagttaagg gtcttgcgct
    15301 gttcaggtgt aagaccgttt cttgccatga aatcgaccgt gtttctgccg agtatcctca
    15361 cgccgtcaag ctcgcccttg ttcataagca ttttggcaaa ctttgcatag tcctctacag
    15421 tagaaacaag acctgctccg cccgactcaa aggcaggagg taccgtgtaa tcagtaaggc
    15481 aaaggtggaa gtgagtgaaa ggctcatttc cgccattttt gtattcataa cactgggcaa
    15541 gtcggctgta cttttccgct gggatataga agcctgtatc attcataccg aggggttcat
    15601 agacaatttc acctttatgg ataacgctga gaactgagcc tgagaaaaca ccgttggtta
    15661 tctctttttg tgcaacagcg ttgagatatt taaaattttc gtacatgata gacctccatg
    15721 ttatgatgtt tgatatatag gattataaca cagttcgggt ggaaatgcaa gattttgtgt
    15781 ggcaacaaaa aaatccgcta cctataaatg atagcggatt gggtgcaggg gcacgtcccc
    15841 tacattgtaa agtttacaaa caaacttatt gagcgggact gaagtttgac aacaaaaaag
    15901 ccatgacggg aaatgcccgt cacggcgagc atcaaacctc tcccagcgta tcatacctat
    15961 gggcgggagc aaaaaaatcc gctaccataa atgatagcgg atcgggtgca ggggcacgcc
    16021 cctgctggca aagaaccgta attttgatag aaaccatcaa agggggtgca acttcggttc
    16081 agaggggggt gcatttttga ttttaggggg tgcagttctt tgccgaaagg agaggagcgc
    16141 cttcatgaaa taatgtgtta agggaaaata gttttttgtt aaaatgagta tgtttttctc
    16201 tacacccatt gcaaaccaga aagaagcgtg gtataatatg acccaataaa ccaaaatggt
    16261 catatttaag ggggcgtggg cttggctttt accgattacg agactgagca gctccgcaag
    16321 gcgttgctga aggaa
//
LOCUS       DAWEMR010000040        16260 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1495_length_16260_cov_13.883740,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000040 DAWEMR010000000
VERSION     DAWEMR010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16260)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16260)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16260
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1495_length_16260_cov_13.883740"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9406..9415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3958 a         3912 c         3826 g         4554 t
ORIGIN      
        1 catcagatat atcatctgta atagatgaat atagtgaata cggaacagtg acatggcttt
       61 gggtcgatac ttacaatgat tctgattcaa cactaccaac gattcaaaat cctcaaaatg
      121 acgaaagagg agtgtatggt atcccgttgt tctatgctgg cgaaaagctc atttcaccat
      181 tagataagtt tgttaatata ataaatgata aacacgctta tttggaagac gaatttgaaa
      241 acataagaaa cggtattaat acaggtaaag atactattaa gcaatctgat attaatatta
      301 taggtattgt tttattgtcc caaaaagatt tgaaccgtag tgccataata aacgaaatcg
      361 ccataaaggg aaatgtctat gtagttaact aaactataaa tttattgtta tcttgtttta
      421 ttgccaaata ctatattttt cgttccacac aagtctgaat aggcattgtg gggcacacca
      481 caacaggcac aaactctatt ggaactgaat aaccgcaagc cctcggaaat gctcttgtaa
      541 gcgtttctga gggctttttc ttttcagcgg ttagaattcc gctccgcagg ctcggatagt
      601 gatgtgtggc atttccgttc gcctgacggt caagctgagg tcggctcggt gatgccgtga
      661 cggagttcat actcacgcac acggcggtag aaagtatttg ctgacatatt cattctccgc
      721 ataaagtccc tgcccgtgat gtgcttggct ctccattccc catagagctg accgaacctc
      781 gtccagtcgg tggggatagg ctttcgcccg gtgtacttgc cctgagcctt agcgatctca
      841 atgccctcac gctgtcgctg tttgagctgc tcacgctcca actgtgagag tgcagcgaac
      901 acggtgagca taaatttgcc cgcaggagtg tcggtgtcaa ttttctcctt gtggctgatg
      961 agcgttactc ccttgtctgt gagggtgtcg atgatgttca gcaagtcctt tgtggagcgt
     1021 cccagacggc tgatactctc gatgtagagg gtgtcaccct cacgcacata gtcaagcatt
     1081 gccctgagct gagggcggtc ggtgttcgct cctgacagct tctcggagaa gatgcggtca
     1141 acctgataac tgcacatgat ctcctgctgt ctcgctgttt cctgatgctc cgtagaaaca
     1201 cggatataac ctattttgct catagttgtt gtttcctttc tttattcaag tgtattttta
     1261 tttctatgtt tagcctgtgt cctctgacgg cttttctcac gctcacgctt catctgctgt
     1321 tcgttgatgt actggcgcac ctgtcgggtg tacttcttcg ccgtttcacg cttggtgagg
     1381 aatgcggtat gctcgacatt aagcctgttc cacttcgatt tcagcttgtt ggacttctcc
     1441 aaaaggtcaa gcatcgtcgg cggtttgccg tcaacagcat acttcttgat atgctccttg
     1501 atgtacttga ccgtctgctc gtagtcctcg atagtggcgg catttttctt cttgaaacgg
     1561 ctctgcgacc agcccgattt gccctgatac tccttgtaaa caggctccag ctcgtcacgg
     1621 tgcttcatct ttgcaatgag cgtgtcgata gctgtaaact tctttttcag atcggcacgc
     1681 tcagttgtgt ggtcatcgga gtgaggtgta ttgaagaaga atccagacag ctcgctgaat
     1741 gacataacgc caccagcttc aaggtctgcg atgatttcgt ttgctctgcc catacctctg
     1801 atagctttcc acggatcttc tttcttttca ggtgctttac tctccgtagg tgtgggctgt
     1861 ggtgtccggg cagacggttc aggcttcgga ttgatagcag ttgcgttaag catatcaagc
     1921 tgtgcctgcc gtcttgctcg tcgggctgca aagaacttct ccacctgttc cgcagcagct
     1981 tttctctttg cggtttcact tgtttcggtg atagtttcag gctcggcaga ttgtttcggc
     2041 tttgtcggtg cggtgactgt ctgtgtcggc ttcggctcgg tctttggaac aacaggcttc
     2101 ggcgcttcga taagcatatc gggagtgaca ttcgccgctg acagcttttc ctgtatctcc
     2161 gctatctgct cggctatgcg ttcgggggtg aagtctgcgc ccagggtgtc ggctcttgta
     2221 aacttctgtt gaccgtcacc tgataaacgg aatttcagct tgaccttatt gcttggcttg
     2281 taaacatatt cgatactgca ctccgaacat ttttcaagaa aatccttgaa gtcacggctg
     2341 taaagcatga cctccgcaat cctgacacgg agcttatctt tccacgactt gccctctttc
     2401 tgcatattcc tctcaaagtg agatacccca tgtccctttt caggctcgga gataacagat
     2461 ataccgtgtt cagcacatat ctcgtccgaa atctcacgca actctgccca cgctctttca
     2521 gactttctgc cttgattatg ctcggtggta aagctcagac cgttatacag atttgtgttg
     2581 ttgaaaatga tgtggcaatg caggtgcgac ttgtcatgat gaacagcaat gacatactga
     2641 taatcgcctt tcagataacg gtcgcacagc tcctctccga tctgcatagc ttgttcgggc
     2701 gtgacttcac cgggggcaaa gctctgtatc atgtggtagg caaggatttg ggtgcgtcct
     2761 gttcctgtgt ttcggacggc tcggaagtcc tcgcttgctc ctgcgctatc ggctcggcaa
     2821 gcataagagt tgacatataa accgtcgatg gtcttatcat ctcttgtgat gtaagcgatc
     2881 gctgcgctga ctgttgccct gatcgtatgg atagaagtgt atgccaaatt tcgtttaccc
     2941 ctttctttat ctcggtaata tcctcggcat agatgtgacc tgtgctgtaa agtcggacaa
     3001 gtatctggtt gatgtttgct ccgatgcgtt tcatcagacg gttacactcg gcaatttcgg
     3061 aacggtcggg cgtgagatca accctcgccc gaacacaatc acggatatac tcggtcttgt
     3121 tcttgtaacc gtactgctca cacttggaaa gaatgtcctg catttccgat tcggtgaaac
     3181 gaacattgag ggtgtaggac ttcttctcct tcttgcgttt caggtacttg cgttcatcgt
     3241 cggtaagctg gctctcgtcc ttgtcggcaa ggcacttgtc aaggtcggct ctgacctctt
     3301 tgtcaagtgc gtgattgatt gctccctcgt aggtcatacc ctcaaactcc atagccttgt
     3361 gtttgggcgg tcttccacgc ttctttttct cggttttcat agtggtgtct cctttgtttg
     3421 tatttcggcg ttagccgatt ttcttgaccg ctgtgcggtc gtttcggggc ttggggtgtc
     3481 cctaacaagc atttggtatt ttcgctcgct gtgcggcgca aaatacaatg cttgttattt
     3541 ttgttgcgct gtagcgctat tttccagcct gtgggatttt catatcactc aggctttcga
     3601 tgctccgtca gcgaatttgg agctatgcga cttaaactct ccgccgctta ggtcggcggt
     3661 catactcgct cacgctcctg cgtgatgcac tcaatatcat cactcttgaa attgtaaact
     3721 ggctcagcgc agttattgcg cagtctgggc ggcagtccta tgaaatatgc tcccataagt
     3781 taataccaaa ttattgaccg cttcgttaag tataccgcag aaaaatttta tgatgaaaat
     3841 gatatgcagg ggtgcgtgtg atcgtcacaa cgtcaaagcc cttcactttt ctaccggtgt
     3901 ttttgagaaa atggcgttgt ttttcagaaa agtttacact ttgttcatga cggttctccc
     3961 ttgtgcctaa attcaacagc atatttctat acagaatgtg aataggtcac ggcggcactc
     4021 ggataaaacc ccctctatat atacaagcac gttcaatcag gaaacgggca tgatttttat
     4081 aaaaatagtt gctccgtgat aataattctc cgtattgaat aaatcagaaa tggtgaccta
     4141 tgcagtatgc agtcaatgtg atatggctcg gatttgcaat gctcggtctg ctttctgcaa
     4201 aattccccat aacaatttca cgaatgaaga agttttatgg cgcactcaac ttcttgcgca
     4261 atgcgaatag acttttgctg tccgagaatg ctataatatt agcatagggt attatcccta
     4321 aatccaatac attgggaggt gatgagaatg agtaagaaag ctaagaccga ggttgatgag
     4381 aagtccgctg ttatcattac ggatgctgtt cttgcggaga atcaggcaaa gctcgacagc
     4441 tacgaaaagt ctatcaaagc tgtgcaggag cgcaggcgca aggtcatcat tgatagcaag
     4501 ctctacacct acgataagct ctcacagctt tacggcggtg cggagggaca ggcacttctc
     4561 gatgctgtga ctgcggagca tacgctgatc ggcaagctga cagcaagcgg tatgacctat
     4621 gagcagattg gtgagctggc tgacgatagt tctgccgatg ctgacaacag ctcggacgaa
     4681 agctattccg aaaagtacgc taagcagatg agcttttcag aaacagatca ttcctattcg
     4741 gagtaacata tattccgaca acaaaacgat tgctgtgtaa gggacggctc tgccgttccg
     4801 tacagcacct gtgcaggcgt aacgcccctg caacaaaaag ccattgcgtg tagagataga
     4861 taatcgcagg cggaaaggag acacttgcca agaaaagata caggatttac tttggctgag
     4921 ctgaatgaga gatataaaga taagccgacc agcaaagccg tgtacgaagt ggacggtcag
     4981 cgttatgctg tgacaagtca tttcgtgggt gaaaaagacc ttgataaggt gctttatgaa
     5041 cttgctttca aaagagcgtt tcagaaaacc gaaaagaact aaaaagtttt gaaaaaagac
     5101 ttgccatatt cggttttctg cgctataata gttgtatcat cgaatatggt ttgctttgat
     5161 tggaaaggag ttattatggc aaagcaaact acttacaatg tcgggatcta tgtgcgtttg
     5221 tcgcaggagg acgaacgtgc gggcgaatcc ctttcgattg aaaatcagaa gaagatgctg
     5281 acggaatatg tcagcaagca ggaggggtgg aatcttatcg agatatgcga ggacgatggc
     5341 tattcgggaa caagtttcga tagaccaggt atcaagcaga ttcttgatga tgcaaagtcg
     5401 ggaaagataa atcttatcct ctgtaaagac ctttcacgtt tcggaagaaa ttatatcgag
     5461 gtcggtcagt atatagatta catttttcct tcattcaata tccgcttcat cgccctgagc
     5521 gacaatgtag acacgcttga cagaaacagt tcggcaatgg atcttatgcc tgtgatgaat
     5581 ttattcaacg aatggcacgc cgccaacacg tcaaagaagg tacgcagtgt catggcagca
     5641 aattctcggc agggcaagta tctggcggct tcagcttctt atggttacat caaggcagat
     5701 gatgaaaagc atacgccctt gattgatgaa gatgccgccg ttatcgtaag acgtatattt
     5761 gagcagaggg caagagggtt aagtccgaag cagatcgcta tacagcttaa caaggacggc
     5821 atcgcaactc cctcggatca tcgttatcag cttaaaggca aagataatcc ccttgtgact
     5881 tcacatctgt ggaatgattg tatggtgcgt ggcattctta acaatgagat ttatatcgga
     5941 aatctcgcac agcagagagt gacaacggta tcctacaaaa atcacaagca gatacgcaag
     6001 gacaggtcgg aatggatagt gattgaaaat aatcatgaac ctatcatttc aagagagctg
     6061 tgggacaagg ttagagaggt tgacgcatcg gtgagtatcg gcaagacaac aaaagaaggc
     6121 gtgattcttc cgctgtcagg atttatgtac tgcccagact gcggttacaa gatgaaaatg
     6181 aacagaacca tgcacacctc taagaagcgt ggaacatatg agactgtcag ctatcgctgt
     6241 ggtacttatg ttagaagcgg aatggacggc tgcactcctc attccatttt ggaaagagta
     6301 atttcacaga ttgttgttga cgatattaga gcaaaggcac gacttgccgt tgaggacgag
     6361 gacgcactcc gtgaagccgt tagaaaacgc agacaggcta ctgccgatgc ggagagccag
     6421 cataacagca aggaactcca tgagggtgag aaaaggcttg cacagctcga aacgcttatc
     6481 agtaagacct atgaagaaaa gctcctcggt actgtacccg aagaactctg cgtgaaaatg
     6541 ctgaatcagt atcttgatga gcagaagatg ctgagagaga aagtcgctgt actgaaagaa
     6601 aagtgcgaaa ccgctgaaca ggctgaaaag gatattgaca gatacattga aaatatcaag
     6661 aagtatgtcg atattcagga gcttacccgt gagacttgtt tggagcttat cgagtacatc
     6721 gtgattggtg atcgtcccga aagcaaggac gaggacaggc agattcacat cttttacaag
     6781 ttccttgaca aagggcttac ggagaaaaga aacattctgc ttccgcagaa tgtggagtaa
     6841 ataatatttg ggtgtacttt tatggggttc tcctccgtgg gcacaggcac acccgcctgc
     6901 ttgtggctgt aggcatgggt ggcagtcagt tttgcctccg ccgttttcag ggtgtcggaa
     6961 gggtcgccct ttctgccgat gacacagtag taggagtagt catccatgca ggcctcggcg
     7021 gaaaccgaca gcctcagcgt tggggtgttc acgtccacat agtaatcttt ctccggcagc
     7081 ttgtagggcg ccgccgtggg attcgcatta ttcttctgat accactgata ggtcagctcg
     7141 tcgcctttca ggctctttgc cgagacagag aaggtgaccc agttatgttc gctgtacagc
     7201 aggtcgtttt ccggctcttc gtcataatcg ccgctgctgt agatagaggt cttacacttg
     7261 acgcccctcg gctggcggtt gatgacgatg gaactgccgc tgatgtagtc gcagaccttg
     7321 cacacgccat tggcgtcaaa ttcatggttg gcggcattca gttttttatc gtcacaatag
     7381 cggcaggggt gccagtgctg cgttccgtca gccgcccagc tggcggaggc tatgtgggta
     7441 tgtccggcgc cggcatgctc cgtcttcaaa tgatcctcaa aatcagaatc gctgaagcac
     7501 cagtcgtcat gatcgcagcc catagcgctt acggcatctc ggcagcagtc cttgcagcgc
     7561 tgatcgcagc cgtcctcgca gggatcgctg tcgtggaagc acaggccgca gtcctcacag
     7621 aagtgatccg catagtcagt gtccattgcg cacattccct cggagcagtc ggagttgccc
     7681 tcgcagcact ccttgcacat ctcgcaggtt tcgcagaatt cgtcctcgtc ctccttatct
     7741 gtgacccagc cgtcacagcc ctggcagatg tgctcgtccc actcggcgct ttccacgcag
     7801 gtctcgccgt ccggacagcc gccgccctcg gagttatctc ggcagcactc aaggcacagg
     7861 ccgcagtcct cgcaggactc ctgattcagc aggcattcct cgcactgttc gcaggccgtg
     7921 tggcaatcga tgcagtggat gacgccggta tcatcatcgt ccacgcactc gtccaccgaa
     7981 ccgaagcagg cgttacacag cgagcagtgc atcgggtcgc tgtcatcgtt tgcacagtca
     8041 tcgcagaagc cgcaggagtc gcagatggtt atggagcagc agggaccctt atggcattcg
     8101 ccgcacagct ggtcatcctc gccgatgtag cacttggcgc aggtggagca gtgcgtgtcc
     8161 tccatgcagt ccgcgcattt atagcacgtc tcgcaccaat agggcgtgtc tccaaagcat
     8221 acaccgcagt cgttacaatg ggtttcaacc cagcactcat agttacagtc aatggtgcag
     8281 aacaggcagt cacacatata ctcgctgcca aaatggtagt ggttgcagtt aggacaatcg
     8341 aagccgtcgt cgtagtcatc ccaaaaggca tgggcgggaa gctctagact cggcatcagt
     8401 gagagcagca tcacaagcgc caacaggacg ctggcgacgc gccttcgttt gttcgttttt
     8461 gtcatactat tattcctccc tcataattat tttgaagatt tttatgcttt ttgtgttgcc
     8521 tgatttaaac actgtcgatg accgaagcaa agtagttttc ggataccgaa ccgaagagaa
     8581 aagcgtctat cagaccctcg cgctttgcct gccgtacctt ttcggtcaaa tcttcatctg
     8641 tatcatcatc cacaaagaga atgacacgac agctttccgt gttccgtttt accttgtcac
     8701 ggatcgccat tcgttctttg aacatccatg gcgaatacgc cttgacctcc attaaaagca
     8761 catccggtcg atgcgttttg caccactcgg cggttgcgtc cggcgactcg gaaatcacaa
     8821 cttggcaatc gtccaatttt tgcatcagca ttcgctccat ggtgtgcgca tggatatcgc
     8881 tttgaatgtc cagaacgatt tttttcatca ttcatcacca ccttctaaaa gcacaatcct
     8941 aatcttacat tatctattat actgattttt tggcgtttgt cactggcact tgtgccagta
     9001 acagaagata aatcccgatt ttttcaaaaa tttttgcctt ttgggcgcga aaaacccctt
     9061 gaccgtacag ccaaggggtt cagttttgct tattcgtctt ttaaggtgat caccatgagc
     9121 ttgttttcaa tggcggttgc aaccagcgct tctctgcttt caaagccgct cttttccacc
     9181 atatcgtcca gattccagcg aacgccgttt ttggagatgc ccagcttatc ggcaatttcc
     9241 tgataggtaa agccttttat ataaaggcgt aggatgtcta actgccgggg cgagaagaca
     9301 tcggacatcg taccctccat ttcaatggcc ggagcggtat ctgggaatac cttttcgcct
     9361 gcaagggtgc gctttaccac gctcatcagc tcctctgttc cgtggnnnnn nnnnncctta
     9421 taccacaggc tgtcggcagc acccattttg gctctttgca aaacttcggg atcgacaagc
     9481 gaggtagcca caacgatttt gatttgcgga aaagcctttt tgatgcgctt tgcggcggca
     9541 agaccggaat gcttatgctg cgtctgcaca tccataagga cgagctttac ggtggcgtcg
     9601 cagtgctttt ccgcttcaaa ggcgtcacgg aaaacgccga caagatgaaa ctcgccgtct
     9661 gccgcaagac gatcgacaaa gtgcttttgg atgtataaat catcctctac aagtatggtg
     9721 tttatcatag gctcatctcc tttgcgggaa tcgtaatcag aagagcaaag cgtggattgt
     9781 gcgaaatatg catttcaccg ccggagcttt caatactgcg gcgaagcgtt gacaggccgc
     9841 tgccctcctt tatcggttct gtgggtattt tgccgttgtt ggttatggta acatcatagc
     9901 gttcgcctcg ctgggcaatg cggatatata cctcattccc gtctgcgtgc ttgatgcagt
     9961 tggtcacgca ctccttggcg gcagaaacgg tcagctcctc ggctgcggta tcacggggca
    10021 ggtagccgtc gatttttaca gccacgccaa gctgctgtgc agttcttttt acttcgtcca
    10081 ttgtgctgac agaggtgaca cggttgcttg ccagcatacc gatggcattt tgcagcgccc
    10141 caagctttcg ctcggtatcc tctccgctgt ctatgatgtc acggatggtc aggaggcttc
    10201 gcccgatggt gtcgtgaata tagaccttta ggtcaaggct ttccttctct ttgacatcgt
    10261 tctccatgcg agcgtacatc ttcctgagct ttcgattgac ttctgccagt tcttcattta
    10321 tttcctcttg cttttggtaa ccgttataca gctcggttac attgtgcgcc gttatctgct
    10381 gccagcggtc atcgctgtcc acggtgatat tttgtgtgcg aaactgccag acggtcttgt
    10441 cgggcaggat gtagcaatca tccttgacgg tcacggatct gtccggcacg ctcagggcat
    10501 tttccatatc gcttttgatt tgcagctcgt ggtcgcaaag ggcaaaggag agcctgcgca
    10561 tacggttgtt gcacaggatg atcctgccgt tcgggtcggc aaagcagatg cccataggca
    10621 gcttatcggt tgcttcctta atggagaaag gcgagagctc gtttttttta cggcggtatt
    10681 cccgcggcag agcaatggca aagtgaactg ccgcaaggaa aatggcagcg gcaaatatca
    10741 gccacggtgc gtccagcaga aatgcgtccc cgatattatc gggatctgcc tgagaaacgg
    10801 agaatagcag cgggatcatc accgccatga ctgccagact cagcaggctg cgcacaggct
    10861 tattgctgcg aagaagctga tacataaaca gcccgatctc aaggacgatc tccacaagca
    10921 tgataaacgg cagcatggtc tgccaaaatt cagaaagaga agaaaatgca ctcatttcct
    10981 gtcaccttct ttcgcttcaa atacaaggtg ctggtattcc tcgtcatcct ctttttcaca
    11041 gcggatattg tctccgctta ccaaagcgga aaggtcggct ttgcagcaca cattcagtga
    11101 caagcgcaag ccgcctgcct ttatgaggct tacctgtatg cctgttaaat tcaaaatgtc
    11161 ggcttcaata accgattcat aaaaatcaaa tacagcagtc gccgttttgc cggagagcat
    11221 ggaaagagat tcgtccacat agagcgagct gcggacgccc aacagcttca gcgtttggag
    11281 ggactcattg aatgcacggt ggagttccgt ttccgctatt ttaatatctc ggtcggtaag
    11341 gagcgtcaga tgcttgcggc gtttaatata gctgcacagc acggcgatct cggcaaggag
    11401 cgttttggca ctgtcgggat cggtgcttga gattcggcgg tattccttag tgagctctgc
    11461 aatgcggtcg atctgcgtct gcgtagcgga acgcaggaga tcatacaggc ggttttgctc
    11521 ctccaccttg cggcgcttgg cttcacgctt gtattcataa cggagcattt cgttcctgtc
    11581 ggcaagctcg tctgcaagaa gctcggagtc ctccttgatg tcacgcaaag ccgacacatc
    11641 ctctgtccat acggcatagc cgccatcaat gggcatggtg tgaacggtca agccgcccat
    11701 cgtgaccggg ctttgctcgg cttttttcat cgcttccttt gagataggcg cagtgtttac
    11761 agctgcatac cgaacggtaa agtccttgtc ggtgatctct gccgatgtgc cgacagagga
    11821 ggcaaacaag tccacataac ggctgtttga atgtatatat ccacaggcga tgcaaagctc
    11881 aaaggtcaga atatacatca cactttggaa tgcggcaata tcgccgaaca agtggagcag
    11941 ccaatccgca cccgcatagt aaagcacgct gtaaacgatc gtcagcagca taggaacgat
    12001 tggcagaaag tggagccttc ctttttgcac acggcatttg aaaaacataa ttacgagtgc
    12061 ggcaaaggca cacagcacct gccagcctac aataatgaag tagcctatcg agtagccgtt
    12121 gtttttgtct gtccacacgg cggcgtcctt cggaaaggta aatacgaatt ggtgcaggtc
    12181 atttgtgagc acgagcagaa gcaatgtgcc ggagatgatc cacagaatcg acattccttt
    12241 gggaaatttg tattcgtccg gctttcccaa tgacatggca atcagcagcg ccagcatagg
    12301 cacaaacagc atgggcagat agaacagata ccacaggtaa cggacggcat ccggctgcca
    12361 gaatataaaa tactttgccg aacggactac catccatatg atgagcagta ccgcggtgac
    12421 cgtcataaac ctgcacacct gcttttgcac gatccgctga cggacggaga gcccccaggc
    12481 ggcgaacaga ccgatataga taaagctgcg cagataggaa aacagcatcg ggtagaagct
    12541 gcccctgccc acaattcgga agcaatatgc cagtatcacg gcaagtacag cgatgctgct
    12601 tgttatgata gtattctttt tctgttcggt cataaacaga ccccatttcg ccttcattcg
    12661 tgttttgttt acctttgggt agcaaaaaag cccgatatat tcgggctttt cgcaggagac
    12721 atcttttttg tctccacatt atggcgttcc cgaggtgatt cgaacacccg acctaccgct
    12781 taggaggcgg tcgctctatc cagctgagct acggaaacac attacaatcg ttattatatc
    12841 acagttttgc aattttgtca aatgtttatt tgacaaaaaa agttggctcg ccatagcaac
    12901 aagtatcgac gcacttgcag tcggaataac gttcgctttc ctgaatatct ttcttgaggg
    12961 agttggcata ctcaaatagc ataaaaacaa gggcggaagt tttcaaacta tccgccctct
    13021 tgttatgact gttagtcccg ccgactatga tgtttacgct tttgcgtgta catctcacga
    13081 tctgacgctt tcataagatc gtcgatctct ttcagtgttt tcggctttcc agctttgtag
    13141 ccacagctta tcgacacatc atatggcgac ttgcttctgc gattgtattc tgcaatgctg
    13201 cgggatacct tttcgtaaaa gctgtctgca ccgtctgttt cagcgataac agccacaaat
    13261 tcatcaccgc cgaagcgtga gcatatccca ccttctgcag tgcattttgt catcagcgac
    13321 gccactgcct ttatcgcctt gtcaccctcg ttgtgaccat agaggtcatt tatcttttta
    13381 aggttgtcca tgtcaatgga gaatacccat acgcctacgc cctgtttctc agcctttgaa
    13441 ataagctttc ttacgttctt gtaaaagccc ctgcggttat atatccccgt catggggtca
    13501 agcatatgta tccttgcaag ctccgcattt gccctttcaa gccgctggcg gtttttaaag
    13561 ctctccatta tctgattggt gttgtttata aaacgtcttg tgttctggaa aagaaaatat
    13621 ataggactaa atgaatttgc cgcatagctt atgacctcgt cctgaaaatg aagcggcgag
    13681 aacattaagc agttatggcg tccaaggact tgctccatat caggcaaaag atccttggtg
    13741 tcaaatactt caccggtctt gtatttgttg tcaaaagtct gcatgaaaac tctcatctgc
    13801 tttgtaaagc tgttattaaa gcgttctctc tgcctcgtgt ttgacagaaa atcagagttt
    13861 gtgcataccc agctataata atctcccgaa cgtgccatta cctttgccat ttcttctacg
    13921 gtgtgacatt ccactgattt tgcaagataa gagaacatat gctgttcgtg accatcagaa
    13981 ttgttcattc tatggtaaag ctcaaggatc ttgtcgccta cttctctcgg gtctattttc
    14041 ttacagccgc aagactggga tatcctcatg gtgtagctga cctctatttt ttcaggaaga
    14101 ggcactccgt tcatcatatg gtctatggcg tttattatct tttcgccaag cacatcattg
    14161 tctacagccg ccgttgtaag acgagggctg ttatactgtt caagctcgat gccgtcaaag
    14221 cccgttacaa taacgtcttc aggcactttt atgccgcgtt ctctcagtat gctgcaagct
    14281 gctatcgcca tagcgtcgtt tgcacatatt atcgcctgcg gaaattccaa actgctttct
    14341 aaaaatttct ccaatactcc ttttgttggc atatcccaga agtcaccata gccaagacgc
    14401 tcaggctcaa attctatgcc gttttccgca agcacctttt tgtatgcttc tatacgctct
    14461 tctgagaaag tattgccctt tacgccagca atgaaattaa ctcttctgca gccgtgaaat
    14521 tcgatcacat ggcgaactat cttttcaaaa acgtcagagt aattgaatgt cacactgcag
    14581 caattatcca ttgtactgtc aacgcatatg acaggaacac catgttcatg ggcacgcttg
    14641 cttatctcgt tggaaatgct gtctcttttt attgcctcgg gcattattat aagtacgtca
    14701 agaatgtcat aatttataag atcaaaaatg cttgcttcac catggttgta ggaaatgttg
    14761 tgataaaggt cgcaaaaggt gttaaaaaga agcaccttgt agccctcttt tacacccttt
    14821 cggcttatgg aagatactat atctctcaca tagtcagatt gtacgcatga agtgcagata
    14881 cctattattt tatatttcgc tgtatcgctt ataaacatag cttgctcctt cgtcagggtc
    14941 atgatgaccc accactatct atataaaata atcgcttttt tgctttgata tgtgactgct
    15001 attagaaaat attcacatac cgttaatttt accatatccg cactctaatg tcaatagaaa
    15061 actcacaaaa ttcacaaaaa tcttcttttt gaaagcattt attctgttgc aaaaaacaaa
    15121 gaccgacttt cagctttttt cgctggaagt cggtctttgt ttatcgtaaa acatatcgag
    15181 caaggggcga ccagaggtcg cccctacaca aaatagcgta ttttatgggc ttttttattc
    15241 agctacccac tgttttaaag ccgcagaaag ccgtctgtcg atatcctttc ttgtctgctc
    15301 gcactcctta ggatagtcct ttgcagcctt gaaaataaat ttcattacga atttaagtcc
    15361 cacaggaagt gcttttctga gcattgtctc agggagcaca aagtgcgttc catgctcgta
    15421 agtcagggct tcgtaatcgc actcgtgagg gcgttctttc aagcgcttat ccatgcggcg
    15481 gacgtatttt cctgcctccc agaagctgtc gtcatcagca cccaccatta taagcttgcc
    15541 cttgatgttc tcaaccttta tcatttcttc ctctgtatgt tctcttgcct tttcagagtc
    15601 gataaaaagg tgcgtgcttc gtgttatatc tcccgagcct ttggtttctt cttctatttt
    15661 gtgatagtat tcagggtggg cataaacgaa cggcatatag gcaagcggct tgccctgcca
    15721 tgaaagtgtg gaagcgtcag ggataggcca ttccttgcag ccgtcttttt cgccctgctc
    15781 aaagccctgc catacaaagt cgcttggcgt aagaccgaaa gtgagtgtta tgtcagggaa
    15841 atatgaagct gccacaaggg aatccattcc tgccgtactc attcccatta tgcctatctt
    15901 cttgtttcca tggcttttga gccactttat agcggtctct atcctttcaa gcgggaagtt
    15961 cacatggccg tagttcttca cgtcaggtga catacacata acgttcacgt catttttgtg
    16021 aagccatttt gctccgcact ttgccataaa gtcattagga tcgtcaccga acagacctat
    16081 cattgcgcag tttgcatcct ttgggttttc ataatatgta ccataaaaac cgtctttctc
    16141 attgctaaaa aattgtttct tcataatacc gacctttcta agctggtttt ctttataaag
    16201 ttagaataaa ctaaccatat ctcaaaaaaa cagggtggat aacttcgccc taagctatat
//
LOCUS       DAWEMR010000041        16105 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1508_length_16105_cov_11.708972,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000041 DAWEMR010000000
VERSION     DAWEMR010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16105)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16105)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16105
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1508_length_16105_cov_11.708972"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4758 a         3676 c         3448 g         4223 t
ORIGIN      
        1 cagggcttgc aacaacaagt ccgtcagcct tctcaaactt tggtgcgatt tcatttacca
       61 tgtcattgaa tacgcatttg cccgtttttg aacatgacct gcaggcaata cagccgcgaa
      121 tatccttgtt cccaatttga acagtttcta cttcaacatc actctcttta aaaacctttt
      181 ccatttctcg aatggcaata gaagtatttc cgtttatcct cggacttccg tttaaaataa
      241 gaacgttcat gttaaatcct cctatgtatt tattttaata attatatcac ttggagtaca
      301 ctataagtca agtgaatttt tatatgcttt attctgaaat aattgttaat atgagcattt
      361 cctgaaaaat ccgccgacaa aaagtacggc ggatatgata cagctatata tattgcaaag
      421 cgtaaaatgc ccgtcacggc gaacatatag ctttcccagc atatcacgcc tatgggcgga
      481 aataaaaaaa tccgctacta taaatgatag cggatatggt acaggggctc gcccctgttg
      541 gtgcgcctga caggaatcga acctgcgacc tcaagagtcg gagtcttgcg ctctatccaa
      601 ctgagctaca gacgcatata aggagcggaa atccgctcct tgttttttta taaatttttt
      661 atctaagctt agctccgcat ggcaagtcct gatccgggaa aataacgctt gccgcaccgt
      721 caggtgtgtc agccgcacat atcatgccgt ttgactcaac tccgcaaagc ttcacaggct
      781 tcagattggc tacaataact accttcttgc caataagatc ttcaggcttg taccatgccg
      841 caatgcccga tactacctgc ctgccgccaa aaccatcgtc aagctgtaag caaagaagct
      901 tcttggactt ctttaccttc tcgcagtcct taacaagagc aactctcaga tctacctttg
      961 caaaatcatc tatggttatc tcgtcagcta taggcgcagg ctcaaatcca tcgtcagcct
     1021 tttcctcagg ctctgcattg ctgcctatta tcttgttaag ctcctctatc tccttgtcaa
     1081 cgtcaattct tgggaagatg atctcacccc tgtgaactgt aacgtcagca ggaagtacac
     1141 caaacttgcc agcgttctcg tatgtgatgt cgctctcagc cgcacctatc tgctcccata
     1201 cctttggcat tgttgtaggc ataaagttgc tgagaagcgt tgatactatc ctgatagttt
     1261 caagcagatt gtaaagaact gtcgcaagtc ttgccttttt gctctcgtcc ttgccaagta
     1321 cccatggagc tgtctcgtca atgtacttgt tagccctgga tactaccttg aatatctcag
     1381 ccaaagcatt gtttatctga gtcttgtcca tgaactcgtc gacttttgct ctaagctcct
     1441 cagcctcagc aataagctca gcgtcaaaat cgccagcctc tctgtcagca ggaagcgtgc
     1501 cgccaaaata tttgtcagcc atagctacag ttcttgaaac aagattgcca agaccatttg
     1561 caaggtcaga gtttattctg tttatcatta tctcgtttga gaatgagctg tctgcaccta
     1621 aagccatttc acgaagaatg tggtatctga tagcgtcagc accatatctc tcaccaagaa
     1681 taaacgggtc aacaacgttg cccaaagact tggacatctt ctgtccgttg aatgttatcc
     1741 agccgtgtac agcaagatgc ttaggcagtg gaagatcaag tgccataagc attgcaggcc
     1801 aaacgatagc gtggaatctc atgatatcct ttgctaccat gtgaacgtca gcaggccaga
     1861 acttgtcaaa atcattgtac tgctcgttcc agaaaccaag tgcagaaata tagttagaaa
     1921 gtgcgtctat ccaaacgtaa acaacgtgct tgtcatcaaa tgtaacagga ataccccact
     1981 tgaaagtagt tctcgataca caaagatcct caagaccggg cttgataaag ttgtttacaa
     2041 gttcagtggc tcttgtcttt ggctggagat aatctgtctc agtgagaagc ttctctatcc
     2101 tctcagcaaa tggtgacatc ttgaagaagt aagcctcctc cttagcctct gttacctctc
     2161 tgtgacactc aggacaacag ccgttctcat caagctgtga ctcagtccag aagctttcgc
     2221 aaggcgtgca gtatttgccc ttgtattcgc ccttgtaaat gtagcccttg tcataaagag
     2281 ccttgaagat cttctgaaca gtttcaatgt gataatcgtc agtagttctg atgtatctgt
     2341 catagctgat gttcatgtag ctccaaagct ttttgaaagt cttgactacc tcatcaacat
     2401 attccttagg tgtaacaccc ttttcagcag ccttctgttc gattttcaga ccatgctcgt
     2461 cagtgcctgt gaggaacatg acatcatagc cctgcatacg tctgtatctg gcaatagaat
     2521 cgcaggcaac agtggtgtag cagtgaccga tatgaggatt accggaaggg taatatatcg
     2581 gggtagtgat ataaaacggt tttttctcca tataaaggac ctccgtaaaa gaatttgcat
     2641 aagcagcatt tcttatatta tactacttta cagtggtttc gtcaagcggt aaagcgattt
     2701 aagtgaggag tgaggaatga ggaatgagga gtgaggaatg agaaatgagg aatgagcaaa
     2761 gctgaccaaa gaaaagaaca agcataaagt aaaaaacagc accgagcgta gcgagcactt
     2821 aaacgcaggc tcaagcccgg ctttagaggg cttgcagggt aaagggacag agtcccttgt
     2881 gggttcttag ggcaacgccc tgagcggagt ttgaggcaga gcctcattcg gcgcaagccg
     2941 acaaggtgca gattaaagtg taaaaagcac acgagagcca tatatcaaac aaaccccata
     3001 tcggagcaca aaatctgcac cgccccccct cgccctgagc aaatgtaaat ccgcaaagag
     3061 cgtagcgatt ttgcggtaca acaaacttcc cctaacaatc tgtatgggct ggcgccctcg
     3121 acagcccacc cccttgacat ttcgcattat tgtttatctc acaacccttg ataaccccac
     3181 aaacatcggt aggggcgaac atcgttcgcc cgtgctcaga acttgcataa attctgaatc
     3241 atagaggaca ccacccgatt tgtgctgatt taacaaataa ttcatacaac tttcgtataa
     3301 aaatgctata ataaaaaggt taaagtatat attctaagaa aaagtaactc taaggtggga
     3361 atcgtaaaat ggaagaagta agagaaaata aaatgggaac gcagcccata ggcgagctgc
     3421 tgttcaaaat gtccctgccg atcatgatat ccatgctcgt gcaggctctc tataatatcg
     3481 tcgacagcat attcgttgcc aaaataagcg aggacgcact cactgccgtg tcactggctt
     3541 tccctgtgca aaatcttatg atagccctcg gtgcaggcac tggtgtcggt atcaacgcac
     3601 tgctttcaaa atcacttggt gaaaagaact tcaagcaggc taacagcgcc gccaataacg
     3661 gcattttcct ctgccttgtg aacttcgtta tcttcgtggt tttcggtgca tttttcaccg
     3721 agatcttcta tcgttcacaa acctcaaaca aaataattat cgaatatggc gaccagtacc
     3781 taaccatcat caccctgcta agtatcggtc ttttctgtca gatgactatg gaaaaactat
     3841 tgcaggcaac aggtaaaact atctattcaa tgtatactca gggaataggc gcagtgataa
     3901 atattatcct cgacccttgc tttatctttg gcgtgggctt cttccctaag tggggcgtaa
     3961 caggtgcggc tgtggctacc tgtatcggtc agttcaccgc tgcagccttc ggacttttct
     4021 tcaatatgaa gttcaacaaa gagataacca taaaccttgt gaagtataga ccgcaggcaa
     4081 aaataatcaa gaagatctac gctgtcggtc tgccgtccat cataatgcag tctatttctt
     4141 ctatcatgac atacggcatg aataaaatac ttatcggctt ctcaaacacc gccgccgcag
     4201 ttttcggtgt gtatttcaag cttcagagct tcgttttcat gcctgttttc ggacttaata
     4261 atggtcttat ccctattgtt gcctataact atggtgcaag aaagaaaaag cgtatcacag
     4321 gtgcaataaa atacgccatg atatattctg tgtctatcat gatagtcggc actatcataa
     4381 tgcagatttt ccctgcacag cttctgaaac tctttgacgc aagcgataat atgctaagca
     4441 taggcgtaac agccctgcgt ataatcagca cacacttctt cttcgcaggc tttgatataa
     4501 tcataatctc atcattgcag gctcttggca ggggactttc ctctatgctc gtttcattta
     4561 caagacagct cgttgtgctt cttcctgtgg cgtatcttct ctctcttaca ggcaagcttg
     4621 acgctatctg gtgggcgttc ccgatagccg aaatattctc aatcatggcg tcattgttct
     4681 tccttagaag agcctacaac gctgaaataa aaccaatggc agactaacta ggaggtattt
     4741 ttatgcggct tatttttatc agacacggcg accccgatta cgttcatgat acccttacag
     4801 aaaagggcaa gcgtgaagca gagcttcttg cacagcgtgt aaagaattgg gacgtttcaa
     4861 agttctacgt ttctcccctt ggcagagcac agctcacagc ggaatattcc ctgaagcttc
     4921 gtggcgatac agccgaaacg ctccaatggc tgagagaatt taacggcata atacccacct
     4981 ctgacggagg acatcgtatc ccatgggata tgtaccccac cgaatgggcg gataaagaga
     5041 tatactatga ccgcaataat tggttcaagt ccgattatat gcaaacaggc aatgttgaag
     5101 aacagcttaa atgggtgcat gagggtattg ataagcttct tgccgatcat ggttatatcc
     5161 acgagaacgg tatgttccgc attgaaaagc acagcgatga aacccttgtg ttcttctgtc
     5221 actttggcgt atcaatggtg atgataggac atatccttgg tatttccacc atgaatatgt
     5281 ggcatggctt caatatgcag cctacctccg ttacagtcct cggctgtgag gagcgtgagg
     5341 gcgactatgg ctttttcaga tgccacgtca tgggctgtac aaagcacctt gcaggcggca
     5401 atgaacctat ctccaacgca ggctacttca ccgacgtttt tcaggattaa ataaacccgt
     5461 aagggtgact ttttcgctcg catattattg catacaaaaa tatcctctat ccgctctgtt
     5521 atttcagggc ggttatttta ttgtgcgaaa ccttagttta agataatata acagttaaag
     5581 aaaacgtttt cttctaaata tgactatcat tagataacaa catattcata atttgtgaaa
     5641 aagcacaaca aaagcttcgt gttttcgttg attttttagc ttgctttatg taaactaaat
     5701 gcttttaccc ccttgacttt attttgaaaa gggcttataa tatcccatag cttaaaagag
     5761 tatgggtttc atatttttat atgtgcctaa gagagcatga agattagaag aataaaagga
     5821 gagtgaaaga atatgttcca gcttcaatgg atatggcaca atctgaaagg ctaccgaaaa
     5881 ttctatgtaa cagcccttgt gctgtctatc gtatgcaacg ccttgtatct taccacacct
     5941 tatttcagca aagagcttgt ggataagttt ctttcaagca aggacgcact ttttaatctt
     6001 gagcatgaaa gaaagctgtt ctggttgctg cttgcaggca tggtgggctt taccctgctt
     6061 agaactatct gtcagtatac ctgcaacatg acctatgaat tatcatctca gggcatgata
     6121 tacagaataa ggacgcacct tttcagacgt atcgaaaatc aggatatgga gttttatgac
     6181 cgcaaccgca caggcgacct tatgacaagg ctcacaggcg accttgatat ggtgcgtcat
     6241 gcagtggcat tcgtttttaa aggtatgctt gaatcatgtt cgctgtttat agcctcttca
     6301 gtttatttct ttatggtaag ctggaaaatg gcacttcttc tgctggctgt tacaccgttc
     6361 atattcggtg tggtggtgct tcttgataaa aaggcaaagc cggtctacgt tgacctccgt
     6421 gagaagctgt cccaaatgaa taccgacgct caggagaaca tctcaggcaa cagagttgta
     6481 aaagccttcg ctcgtgagga ctatgagata gaaaagttcc aaaaagccaa taaagaatat
     6541 tctgatgcca acaaaaaagc gtcactgctg tggcttaaat tcttccctta tatggaagct
     6601 ttcgcaaacg ccttgtcagt tgtaatgctc cttggcggtg gatttttcct tataaaaggg
     6661 gaaataacaa tgggcgagta tgttgctatc tcaggactta tctggggcat cgcaaaccct
     6721 gttagaaacc ttggtatcta tgtgaacgat atgcaaaggt tcatggcttc cgcaatgaag
     6781 gtcatcgaga tatactatgc aagacctatc atcaccgacc gtgaagacgc agttgaccag
     6841 acagaaaggc ttcacggaga tgtcgagttc aaggacgtta cctttaagtt tggcaagcat
     6901 atagtccttg acaacgtgaa cttcaaaata aaagcaggtc agacagttgc cataatgggc
     6961 gaaacaggtt caggcaagac ctcgcttata aatctcatac caagatttta tgataccaac
     7021 tcaggagaag tcctcgttga cggtgtgaat gtaagaaagc gtaagctctc acagctccgc
     7081 tcacaaatag gtatggcaac gcaggacgtc ctgcttttct ctgataccat agacggcaat
     7141 atcgcatacg gcgactctga tatgtccgaa gaggacgtta agcactatgc aaagcttgcg
     7201 gcggcaaacg aattcgttga gaaaatgcct gacggctatg aaactatcgt gggcgagcgt
     7261 ggcgtaggtc tttcaggcgg acaaaagcaa agactttccc ttgcaagagc cttagccata
     7321 aagccttcca ttcttatcct tgacgatacc acctccgccg tggatatgga aacagaaaag
     7381 tatattcagg acagccttag aaacctcgac tttccatgca caaagataat aatagctcag
     7441 cgtatctcct ccacgaagga cgccgacgtg ataataatcc tcgatgacgg caagataacc
     7501 gaaatgggta ctcatgatga gcttatcgcc aaaaagggat actattacga ggtgttcaag
     7561 ctgcagaacg atggcttcgg aaaggaggcg gtctaaatgg caagaaaccg ttatgacgtt
     7621 gacgaagtcc ttgagacccc attcgacttc gcccacctga aacgctcctt tgtgtatatc
     7681 aaaaaatata agggcaaaat gataaccgcc cttgtgctga gcgtgttcgc cgcaatatca
     7741 ggacttctcg gaccgctcat cacacagtac gctcttgata ataccatacc ccagaaaaat
     7801 atgggacagc tcgtcctgct caccctcgca tttgtcggca ctatagccgt gtccataaca
     7861 ttctccacta tccgttcaag gataatgaca gttgttggtc aggatataat cttcgatatc
     7921 cgtaccgacc ttttcaagca tttgcaggag cttccatttg agtattacga taaccgccca
     7981 cacggcaaga tcctcatcag agttgtaaac tatgtaaact ccgtgtcgga tatgctctca
     8041 aacggtatca tcaatgttat actcgagtgc cttaatatgc ttttcatcat gattttcatg
     8101 ttctttgtga atgtgaagct gtcattggtg gtgctttcgg gtctgccgat atttgcagtc
     8161 ataatgatga tgataaagaa aagacagcgt aaagcatggc aggacgtttc aaacaaatcc
     8221 tcaaacctta acgcctattt gcaggaaaac atcacaggcg caagagtgac ccagatattc
     8281 acccgtgagg aagaaaacgc ccagatattc gacagacttt ccgagaaata tcgtaaaagc
     8341 tggataaacg ccgtgaaata ttctaacctt gtttggcctg ctaccgacaa cgtttctact
     8401 cttgtgagag ccgcaatatt cgtggtgggt ctgctggtgc tgacacctgc ggcagtgagc
     8461 cttggaacta tcgttgccat gacaagctat gcttcaagct tctggcagcc tatcatgaac
     8521 ctgtcaaaca tcttcaataa cttcatcaac aatatcgcct accttgagcg tatctttgaa
     8581 acccttgacg agcctgccac tatcgcagac aagccgaacg ctcaggatat cggtgatatc
     8641 acaggcgagg taaaatttga tgacgttaca ttcagctatg agcagggcaa aacagttttg
     8701 gagcatatct cctttgacgt taagccgggc gaaagcgttg ccctcgttgg accaacaggc
     8761 gcaggaaaat ctacagtagt ttctctcctg tcaagatttt atgacctgga cagcggtaaa
     8821 ataaccatag acggcaagga tataagtcag gcgaccttgc attccctccg ctcacaaatg
     8881 ggcataatgc ttcaggacag ctttattttc agcggcacta tttatgataa tatccgctac
     8941 ggcagacttg acgcaacgga agaagaaata agagaagccg caaagatagt ctgcgcagac
     9001 gagttcataa gcgaaatgaa agacgcctat atgaccgagg tcaacgagcg tggctcaaag
     9061 ctctcaggcg gtcagaaaca gcttatctcc tttgcaagaa cgctcctctc cgacccgaaa
     9121 atacttgtcc ttgacgaagc aacttcatca atagacgcac gaacagaaaa actccttcag
     9181 cagggacttc aaagacttct tgttggcaga acttcattta ttatcgcaca tagactttcc
     9241 actatcaaaa actgcgataa gataatgtat atcgacaaca aaggcattgc cgagtgcggc
     9301 actcatgacc agcttatcgc aaagaaaggc gaatattaca agctttatac cgcacagcat
     9361 atggacgaat aaaatagcat aaaaacacgg gcgaacactg caccctgcaa atgtggctat
     9421 ccacacctgt aggggcgaac agtgttcgcc cgtttattat ctcaccttgt aattgcgttg
     9481 tttaacatta ctttatcact atcttatcca acgtttcccc taccttgtca tggataacaa
     9541 ggtcgcagga gccgtccatg gaagtcttat cccgatttat cagcacaaga tcatccccct
     9601 gaaaatatct caccattcca gccgctggat aaactgtcag agaagtgcct gcaataataa
     9661 gcgtgtcgca ctgagagatc tctctcaccg cctccgtcac agttttatca tcaagcccct
     9721 cctcataaag cacaacatca ggcttgatta ttccgccgca ggtgcatttc ggaatgcctg
     9781 tgctgtttaa aatatcctcc gcagtgtaga attttccgca gtccatgcag tagttgcggt
     9841 gaacgcttcc gtgaagctcc aaaacctttt tgcttcctgc cgtcgtgtga agtccgtcaa
     9901 tattctgcgt tatcaccgaa gaaagtatgc ctgccctctc catttccgcc agcttgtaat
     9961 gcgccttgtt aggctttgcc gtcagacaaa gcattttgtc acggtaaaaa tcataaaacg
    10021 cctttgtgtg ccgcataaaa aacgtgtggc tgagtatctg ctcaggggga taatcatatt
    10081 tctgattgta aagtccgtcc accgaccgaa aatcaggtat gccgctctcc gttgataccc
    10141 ctgctcctcc gaaaaatact atcctgtgtg acttgtctat tatctgctga agctccattt
    10201 ttatcactcc tatgaaaaat gcggcctata ccgaagcaca gaccgcattg attattcacg
    10261 atattagtcg atcttaacga tccagccaaa ctcgtctggt atcttgcccc actgaatgcc
    10321 tgtcattgta tcataaagca tctgtgaaac agggccgatc ttgttgttgt ttatctccat
    10381 taccttgtcg ccccacttga gatgaccaac aggtgagata acagcagcag tacctgtgcc
    10441 gaatacctcg tcaagcttgc cgttgtcgta agcctcagcg atctcctcga tagagatctt
    10501 tctctcgctt accttgtagc ccttcttctt gcatatctcg atagcagact ttcttgtgat
    10561 accagggaga acagagccaa gaagctgtgg tgtaacaact tcgccgtcaa taacgaagaa
    10621 gatgttcatt gagccaactt cctcaacata ctttctctca acaccatcaa gccaaagtac
    10681 ctgctcgtaa tcctgctcgt gagcttcttc ctgacctgca agcgagatag cgtagttagc
    10741 ggctgtctta gcaaagcctg taccgcctct tacggctctt acatacttgt tctcaacata
    10801 gatcttaaca gggttaagac cggttgaata gtaagcgcct gaaggtgaaa gaataatgat
    10861 gaacagatag tggtcagcag gcttaacacc tacatatggg tcaactgcga tgataaatgg
    10921 tctgatgtaa agggaagcgc cctctgtgtg aggtacccag tcctgctcca ttttaacaag
    10981 agttttcaga gcgtagagca tatcctcttc gtttatctta ggaattacca ttctctcagc
    11041 agagttgttc attctcttaa agttctcctg cggtctgaaa agctgaacag agccgtcagc
    11101 agtacgataa gccttaagac cctcgaatat ctcctgaccg tagtgcaggc acatagcagc
    11161 aggggagagt gaaaggtcgc cgtaaggcac gatcctagcg tcatgccaac cctgacctgc
    11221 gtcataattc ataacaaaca tatggtcagt aaaaatgtga ccgaaaccaa gctttgtctc
    11281 gtcagctggt ttttcctttg gagtagtggt acgttcgatc ttgatatcta acatacagat
    11341 ccttctttct taaattaatg atcggctcgg caagcgaacc tttaccacat atattataat
    11401 acttattgtg atctttttca agaggttttg caaaaaaaat tccttgccac aggaaaaata
    11461 tgcaggataa cattttgaaa actgcatttt tatacaactg tatgaaaact gttgagaaat
    11521 cgtcataaaa ttgtctttat attgtaggaa atactccttg aaaaaaaggt gatagtgttg
    11581 tataataggg tagaataaat gtgaaaagag tatattataa taagggtatc taaggaaagg
    11641 taatgttatg aacgatagag aaagaaagga taatgagctt ggtatagatc ttgatatgaa
    11701 aggtcttacc ctcgacagcg atgaagccgc aaagccgcag gcgcagaacc gccctatgag
    11761 ccatgaggat atggcaagaa tgatcgctaa aaatatggca atgcaggacg gcggctttga
    11821 cgagcctatc actcccgtaa gaggagtttc agactttggc gaggaaacta tcaagcctaa
    11881 aaagcccaaa acaactgaaa cacgcagtaa aaatgcaaag cctgcatcaa agcaaacttc
    11941 aaagagcggc tcaaagaatg gaaaaaagaa gaaaaagaag tcctcgtcag gaaagactgt
    12001 cgcaatagca atatgctcag ttgttggtgt tatgatagtc gcagcaggcg gcttctatct
    12061 gtggggcatg aattcgtata aggatagatt tcttggaaat acatatataa acggcatcga
    12121 ggtcagcgga aagaccaagg cggaagccta taagctagta agcgagcagg ctgttatccc
    12181 aaaagccatc accgttacaa agaaagacgg cacaacattt tccataaagc tgtctgaaat
    12241 aggctataac gataatactc aaaaccttat cacacagtat tatactcagc aaaaccacta
    12301 ttcatggttc tcttctgcca tgaagcgtac ggatttcagc tttgattcta agttcaagta
    12361 cgataaggct aagcttgaaa aggtgctgaa aagaaaggtg cttgactccc aaagcacaaa
    12421 agatcctgag gacgcctata tcaaaaaagg cgacgataac agctatgttg tggtaaaaga
    12481 aaccacgggc gacgctgtgg aggaggataa gatagacgag ctttattcct atgtggaaaa
    12541 tcagattgac gacggtagct ttgacgttga tatctcaaag gcagattgct ataaaaagcc
    12601 tgccataaca gcggatatgc ttgaagagac ctgcaagaag ctcaataacc ttaacaatat
    12661 cgaaataaca ttcgatttta tctacaccac cgaaactctc acaggtgata ctatcatgga
    12721 ttggataacc tttgacgagg ataacgccga agctggctat acagttgatg aagataaggc
    12781 aatggcatat gttgaagacc ttgcagataa gtatgatacc tatggcaagg atagagagtt
    12841 caagaccaca aagcgtggaa ctataactat ttccgagggc cagggctgtt acggctggtg
    12901 gatagaccag caaaagacct gcgaccttat cgtagacctt gttgaaaagg gcgagtctgc
    12961 tgaaactgag cctgtctact atgtaaaccc tgactcagcc tatgagtata cctgcaataa
    13021 ggattggcgt acagccgata aggattacgg caacacttat atcgaggtag accttactgc
    13081 acagcatttg tggtattata aagacggcaa gctcacaatg gagtctgata tagtttcagg
    13141 ctatccaagc aagtcaagaa atacccctgg cggtgtttat aagctttggt ataaggaaaa
    13201 gggcaagact ctgagaggct cagccgacgg acagtcttac gcttcatatg ttgactattg
    13261 gaacaacgtt tccaccatcg gcataggact tcacgacgca tcatggcaga acggcaactt
    13321 tggcggtgag cgttataagt caagcacatg gggctcacac ggctgtataa atatgccttt
    13381 agataaagca aaatacgttt atgaaaaaat agatatgggt actcctgtat ttatgtattg
    13441 gtaagagata atagcaaggc aatacggaca actcgtattg cctttgcttt tagtttcagt
    13501 taggagaatt tatgtacatt acagatgaag acaacccacg cctgccattt ctgcgtgata
    13561 aaacttcaaa gctcaccacc tcaccgggct gttatataat gaaagacaaa atgaaaaaga
    13621 taatatatat cggcaaggcg aaaaatctcc gcaaccgtgt gacatcttat ttcagaaagt
    13681 gtcaggatca cctgccaaag gtgtggaaaa tggtttctca cgtctatgac tatgacttta
    13741 tcgtcaccga ctctgagttt gaagccctgg tgctggagtg ttctcttata aagcagtata
    13801 ccccgaagta taatatcctg ctgaaagacg ataagggcta tagctatatc agggtcacaa
    13861 atgaagacta cccacgtttg caagctgtat tgcaaaaata cgatgacgga gcagagtata
    13921 taggacccta tacaagctcg ttcgcagtaa aacaagccgt tatcgaagcc aataaagtct
    13981 ttatgcttcc tacctgcacc cgaaaatttc cgcaggattt cagaaaacag cgtccctgcc
    14041 ttaatatgca cataaagcag tgcatgggcg tctgcacagg aaaattctcc aaagaggagt
    14101 ataattctat agtccgtcag gcggtggact atatccgaaa cggttcgtca gattcagttg
    14161 aaagactcac cgatgaaatg aacaaagccg ctgaggagct tgactttgag cttgccgcaa
    14221 agcttcgtga ccgtatccaa gccatacaaa aagccgctga aagtcagaag ataattaatg
    14281 acgatattcg tgacactgat ataatcgcca ttgctcaaaa tggcgaaatg gcttgtgcca
    14341 gcgttataat gtatcgtggg ggcagacttt ttgataaggc ttcctatttc cttggagaaa
    14401 aagacgacag cgtaaaaatg cgtgaagatt ttctcatgca gtattattcc tccaaagaca
    14461 gcataccgaa agatatcctc cttgacgagg atatcacaaa cggcgagata atagagaaat
    14521 ttctccgcca gaagtatgac catgccgtga atatctctac tccgaaaaga ggacagtttc
    14581 tccgccttac cacccttgcc aaaagcaacg ccaccgagta tctctccata aaagtcggca
    14641 gaacgagccg tgagataacc gcccttgagg agcttgccaa agcattggga cttcccaaga
    14701 ccccgaaata tatcgagtgc tacgatatat caaacctcgc ctctacaaat atggtggcag
    14761 gcatggtggt ctttgaaaac ggcagaccat gcaagaagta ttataaaaag ttctctatca
    14821 aaacagttgc agagcaaaat gactatgcct gtatgcagga ggtcatagaa cgtcgcttta
    14881 gaaactactt tgataaaaca gacgagggct tttccattat gccagacctt atcttgctcg
    14941 acggtggaaa cggccacgtc aatgccgttg agcctgtctt agagcgaatg ggtgtgacct
    15001 gcccattgtt cggacttgtg aaagactcca agcaccgcac acgagccgtt gccacaggcg
    15061 gcggagaaat aagccttgca aagtgcagag cggcgttcaa tcttgttacc gccattcagg
    15121 acgaagtcca tagagtagcc ataacctatc aggcgcagaa gcataaaaag aatgcccacc
    15181 gacttgagat aaccaaagtc aaaggcatag gcgataaaaa agccgcaaaa ctcatcaccg
    15241 agtataaaac caaagaagct ctcaaaaaag ccacagtaga ggagcttgca aagaccacag
    15301 gtgtaaatat agacatcgca agagagctta aagaaataat agatgaaatg tagtgataga
    15361 attgatagaa aataccaaag acatagacga gcttgaattt ataaacagct tttcccattc
    15421 aggcaagccc gtgaaagacc tatccagaat aaaagccttg ctttccgatc ttggcgaccc
    15481 tcaggacagc cttaaattcg ttcatatcgc tggcacgaac ggcaaaggtt caatggcgca
    15541 gatgttcagc gaggtctttc agtgtgcagg gatgaaaaca ggtcttttca cctcgcctta
    15601 tataataagc tataccgaca ggataaaaat aaacggcatt gatatcccaa agcctgccct
    15661 tgcgaaaatg gcaaagcgtg tgaaagccgt caccgatatg cactgcgacc gtgagaattt
    15721 ctctcagttt gaaataacca ccgccatagc tttcctctat tttgctgagg aaaactgcga
    15781 tgtagtcgtt cttgaaacag gtcttggggg acttttagac agcaccaacg ttataaaaac
    15841 tcctattctc acagttatag gctcagttga tttcgaccat accgccattc tgggcgacac
    15901 cttggagaaa atagcctatc agaaagcagg cattataaaa ccccactgcc catgcgtttt
    15961 gagcgcaggc aacgacatga aagttatccg caccgtcaga gagcaggcgg tgaaagatct
    16021 ttctcagctg gttatccccg acatcaacca gctcaaactt ataagctgcg acgtgttcgg
    16081 ctcagagttt gagttcaagg gacag
//
LOCUS       DAWEMR010000042        16102 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1509_length_16102_cov_13.682433,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000042 DAWEMR010000000
VERSION     DAWEMR010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16102)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16102)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16102
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1509_length_16102_cov_13.682433"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6458..6467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4864 a         3580 c         3708 g         3940 t
ORIGIN      
        1 atagctttta caaaggataa tttcttgaca aaagctttat tgcgtggtaa aatatacggg
       61 aggatcgttg caaaaacatg gtcagtagct tttttgtgcc gtcagtttgc aatgatgtct
      121 ttgtaaaaaa atatagatca tgttgaggga ggcgacgtcg ttttatgaaa aaagacgtta
      181 tcaattacat ttggacagat aagaaaagaa cgatttttgg tctgcctata tcatttacaa
      241 gatattttct cacagaagat aagtttataa cacgcaaggg ttttctcagc atagaggaag
      301 acgagcttga cctttatagg gtcatcgaca aaaagcttgt cctgccgttc ggacaaagaa
      361 tgttcggctg tggcacaata gtaatgaatg tcaaagacgt tgatacgcct attaaggaag
      421 tcaagtccat aaaagcgcca agagatgtgc ttgcacagct tgataagtat atcaatataa
      481 atcgtgatag ataccgtgta aggggcagag atatggtcgg cggtgacggc ggtcattgcc
      541 acgatcatga tgattttgat atcaatgacg atatcatata aagttctata agaataaggg
      601 gctttcgcag atctttctgt gagagccttt tttggaaagg aaagtgtgaa tgttttcaaa
      661 agaatatttt cagtcccttg aaagctcgtg ggacaggctt aagacctgtg aaaagccgat
      721 attcatatac ggcatgggcg atggagcgga aaagcttctt gatgaatttg atagacttgg
      781 cataaaatgc acaggtgttt ttgcaagcga cgattttgtc cgtggacaaa gcttcagagg
      841 gtttaaggtg cagactttct ctcaggtgca agcgcagttt gggaatatca cagttgtgct
      901 tggctttggc acgagcctgc ctgagataat ggagcgtatc gataatattg aaaaaagctg
      961 cgaggtcata gtgccggaaa tgtgcgttgc aggcgatgaa aatttcagca aggaaaagct
     1021 tctctcaatg tattctcagg ctgaaaaagc ttaccgcctt tttgatgacg atatttcaaa
     1081 gcttaccttt gaaaagctga cagcgttcaa gataacagga aagctttctt atctccgtga
     1141 gatatttacg gataaggaca agataaccga gatacttccc cttggagaaa acgaggtata
     1201 ttgcgacttg ggagcataca caggcgacac tgccgcagag cttatctcac gcacaggggg
     1261 aaagtatgag agaatatatg ccctcgaacc tgagagaaaa aactttcaga aatgcctgaa
     1321 aaatctaaaa gccaatgaca atataagcct ttacaatgcc gccgcatgga gtattgatac
     1381 cgagctttat ttcgcaggtg gcgcaggcag acagggcaga gtagatgccg caggaaaaaa
     1441 ggttcgtgca agaagtctgg acagcgttct tgcaggcaaa aaatgcacct atataaaata
     1501 tgacgttgag ggagcagacc tagaagctat caaaggcagt gaatatacca tttcacgcta
     1561 tgctcccaaa atatgcagtg cgttgtatca cagaccatat gattacatca ccctgcctct
     1621 gtatataaac agcctttgca aaggatacaa attctatata cgtcaggaaa gatactaccc
     1681 cgcatgggaa acaaaccttt tctgcgtcca tgacaaataa ggagaaaaat aatgagtata
     1741 acagtaagcc ttaacgataa ctggaaaatg aaaaaagcag gctcgcagga gagctttgac
     1801 tgcaaagttc catgctctgt atataagacc ctgcttgact ataaaaagat acccgaccca
     1861 tattatcgtg agaacgagta tatctcaaca cagatatgtg atgatgatta cgattttgaa
     1921 accacattca ccgcagacaa ggccgcagtg gagtgcaaac atactgtcct gcactttgac
     1981 ggcattgata ccatagcaaa cgtctgtctc aacggcattc accttggcag agtgaacaat
     2041 atgcacagag tgtgggagtt tcctgtccat ggtattatca aagaggtgga caacactctt
     2101 acagtgaaaa taacctcgcc aaacagatac ataaaagaaa tggacgaaaa acgtcacctt
     2161 tggggcgttg actccaccat gacaggctat acacatataa gaaaagccca ttgtatgttc
     2221 ggctgggatt ggggtccaat gctccctgac atgggaatat ggcgcagtgt atcccttatc
     2281 gtcccagttg caggacgtat acttgacgtt tactacagcc aaaagcacac cgataacaaa
     2341 gtgactttga aagccctttt gcatatagaa aaccacggct gtaaaaaagc tatggtggaa
     2401 atatccgacc cgcagggcaa tatcgccgca aaagccgaca taagcctaga ggaaagtgca
     2461 gaatattatg aaacagagct tgtgataaac aaccctcagc tttggtgggt gaacggctat
     2521 ggcgaacagc cactctatag ctgtgccgtg actttgtatg atgaaaatga cgcttgcaca
     2581 gataaaaata tacagcgcat aggtcttaga actttaactg tcagccgtga aaaagatcag
     2641 tggggcgagg agttctgctt tataaacaac ggcgtgaagt tatttgccat gggtgcaaac
     2701 tatatccccg aagaccagat agtcacacgc tgtaacgagg agaaaacaag aaagctcctt
     2761 gcagattgca aagccgcaaa ctataacttt ataagagtgt ggggaggcgg ttattatcct
     2821 gacgagtatt tctatgacta ctgcgacgag aatggtatca tagtatggca ggactttatg
     2881 ttcgcctgct ctgcatatct gctcacagag gagtttgaag aaactgtccg tggggaagtc
     2941 cgtgacaacg ttatccgcct gagaaaccac ccctctcttg gtctgtggtg cggcaacaac
     3001 gagatagaat cagcatggga atattggggc cttcctaagg acgaggaagc aaagcgtgac
     3061 tatcttatca tttttgagga tattatccca caggtgctta aagcctatga cccatgcgaa
     3121 aacaccttct attggccgtc atcaccatca tcagggggag gctttaagga cgcttcctca
     3181 aaccatgcag gggatatgca ctattgggac gtttggcaca acttcaagcc tatcgaagcg
     3241 ttcagagagt tctattaccg cttctgctca gaatacggct ttgaaagcct gcctgatata
     3301 aagacctgcc gagcctttgc cgacgaaaca aagggcgact ttgacctttg ctcaccggtt
     3361 atggcggcac atcagaagtg cgttcagggc aacgagaaga taatgtacta tcttgcacaa
     3421 atggtgagat atccttacga tttcaaacag ctcatctatt cctcacagct cgttcaggcg
     3481 gattgcatac gctcgaatgt cgagcatatg agaagagcaa gaggcagatg tatgggttct
     3541 gcctattggc aggtgaatga ttcaaaccct gttatctcat ggtcaagtat cgactatttc
     3601 ggcaggtgga aaggtctgca ttattatgca aagcgttttt acgctccatg ccttatctcc
     3661 tgcgacgata cccatagaaa aaagcctgtc cttaacatat ccaacgagcg catgacagcc
     3721 tttgagggcg tggtaaaatg gaagcttaga aacaataaag cagaaatttt gcaatgcggc
     3781 gaaaagtccg tgaacgttcc ggctcttacc gcacaaaacg tccttactat cgacctttca
     3841 gacgagctta aaaaccgcac cgaccctcgt gaaaaatatc tcgaatattc cattatggaa
     3901 aacggcaagg tgttgagctt tggcacaacg ctctttgtgc gcccgaaaga atttacattc
     3961 ttagagccaa agataaccta cggcatatca gaaagcgagg ataaattcgc tctgacattc
     4021 tgtgccgaga attacgcaaa aagcgtttgc ctcagcctga aaaccgcaga ttgcgtgttc
     4081 tctgacaact ggttcgatat ccacggaaaa gagcctgtta ccataatgat agacaaagcc
     4141 tccctcagca gaaaaatgac agcgcaggag ctgtccgggg agcttgagat aatgcacaat
     4201 tagggctgtg atttcaaagg ctctgccttg tcaccagcag tgaaaagtcg atgcgatcat
     4261 tagcaaaaca aaagcaatgc gggcgaacgc tgttcgccac tacataaaaa gttatatcac
     4321 acattttgta gtggctggcg tccttgacag cccactgctc tgcatttgct atcttccgtc
     4381 aatgttcccc tgcgtataag tagcctgaaa taaaatttta ggaggaactg ctttgaaagt
     4441 aaaactaagc aaatacttaa agccttatgc actttttgcg gtgctgacac ctctatccat
     4501 ggtgggagaa gtgctgggcg acctgcttca gccaaagctt atgtcaaaaa tagttgacga
     4561 cggcgtgctg ggtcaggata tgaaccttat cataagaacg ggacttctca tgctccttgt
     4621 tcttataggc ggcggagctt gcggaattgc ggcttctgcg ttcggcggaa tagcttccca
     4681 aagcttttca agagatctgc gtgtggacgt tttcaagcgt gtcatgggtc tttcatttga
     4741 acagaccgac aagttcacca caggctcatt ggtaacacgt cttacagcgg atataaccgc
     4801 tattcagcaa atggtagact tcatgctgag gatgttggtg cgtgacagcc tgctgttctt
     4861 cggcggaata atcatgatgc tcactctgaa cgtcaggttt ggtatcataa ttctctgtgc
     4921 attgcctatc gagataatca tgatgatagt aattctcaaa aaggcaaacc catactattc
     4981 tatcgttgca aaacgtcttg acagcgtaaa ctctgtggtt caggagaacg tcacaggcgc
     5041 aagagtagta aaagcctatg tgagagagga tacagaagaa aagcgttttg acgatgcaaa
     5101 tataagcctt atggaatcaa acctccgtgt tcagaccctt atggctatcc ttcagccgct
     5161 tcttatgata atactcaatc tgtctgttat tgccgttata gttataggcg gctggcaggt
     5221 gcaggcacaa gctatgaagg tcggcgaggt catggcggct atcacatatc ttactcaggt
     5281 cttgcatggc gtaatgatga tgtctatgat gttccagacc ctcgcaaagg cttccgcatc
     5341 tgcaaacaga ttgagagaag tccttgaaac tgaccctgtt ataaaaagcg gcagcgtttc
     5401 cctttcagat aaaacagggg gcacagtgag ctttaaaaac gtttcattct cctatcctga
     5461 aaccaagggc agacctgtta tctcagacct tacccttgat ataaagtcag gtgagagcgt
     5521 cgccatacta ggtgcaacag gttcaggaaa atcctccctt gtaaatctta ttccaagatt
     5581 ttatgactgc acagcagggg aagtccttgt tgacggcgta aacgtcaagg actgcaagct
     5641 tgacgagctt agaaagaaag ttggcatagt cctgcaaaaa agcgagttgt tttcaggcac
     5701 agttgaggac aacataaaat ggggcgacaa aaacgctacc catgaggagg tcatctccgc
     5761 cgcacaggca gcccaggctg acgagttcat tcagaagata cctgcaggct atgagggtat
     5821 tatagccgaa aagggtgctt cactgtcagg cggtcagaag caaagacttt ctatatcaag
     5881 agcggtactt aaaaaacctg agatcctcat tcttgatgat tccacctccg ccctcgacct
     5941 tggcacagaa gcaaagctcc gtgcggagat agaccgcaaa atgaacggca ctacccttat
     6001 cataatcgca cagcgtatcc agagcgtaaa atcatgcgac aggatagccg ttcttgacca
     6061 cggcaagctt tgtgcctgcg atactcatga aaatttatta aagacctgcg aggtatatca
     6121 ggatatctac gcttcacagg taaaaacgtc aggaggtgaa gtgtaatgcc tccaatgcct
     6181 ccaatgggac ccggcggaag aaaaccgggc ggacctcacg gtgcaaggct cacaatggaa
     6241 aagcctaagg acgcaaagaa cacccttaaa aagcttatcg gctatatagg caggaacaga
     6301 tatctgtttt tcgcacttat cggtgtaatg ctcatcataa ctctgctcaa tcttgccgca
     6361 ccgtcaatac agcagatagc tatcgactgc atcactctca ccaaaaagaa aacaagcgtt
     6421 gacttcggtg ccctgggcaa aacgcttata gggcttgnnn nnnnnnncag tttatctcct
     6481 gtcctctgtt ttcacctata tgcagggaat attctccgca aagctttcac agtacacagt
     6541 aaagaccatg cgtaaagact tgttctcaag ccttgtaaga cttcctataa aatatctcga
     6601 tacccatagc catggcgata taatgagccg tatgacaaac gacgtcgaga atatctccac
     6661 aactatctct cagtctatag gctcgctcat ctcaggcgtg ctgaccatag tgggaacagt
     6721 tgtcataatg cttctgtatt caccgcttct tacccttatc tcgctgtcta cagtgttcct
     6781 taccatattc gtttcaggcg tactcacaaa gtatatgaag aaatacttca tcaaacagca
     6841 ggtgcttctt ggcgagataa acggcgaagt tgaggaaatg gtagtcggat atagaacggt
     6901 cgtttcctat ggcaaagagc aggacgctgt tgacaagttc gagaaaatga gcaacaatct
     6961 gaaaaaatgc ggcataaaag ctcagatatt cggtggtgta atgggtcctt gcatgaactt
     7021 aatatccaat ttcggcttta tccttatcgc agccttcggc ggcttgttcg caataaaagg
     7081 ctttataaca gtgggaacca ttcaggcttt tatactctat tcaaagcagt tctcacgtcc
     7141 tatcaatgag attgcaaatc agtttgcaaa tatccagacc gcaatagcag gcgcagagcg
     7201 tatctttgag gtcatggacg ccgataagga aactgacgag ggcaaggctg actttgaggt
     7261 ggaaaacgtc aaaggcgata tcgacttcaa gcatatcgac ttctcgtacg ttcctgaaaa
     7321 acaggtgctc aaagaccttg accttgaggt gaaagcaggt cagaaaatag ccattgtcgg
     7381 tgcaacaggc tctggcaaga ccaccatagt aaatcttctc acaagatttt atcccgttga
     7441 cagcggtgag ataaccatag acggtcagag catttacgat atcccgaaag acaagctcag
     7501 acgttccata gccatagttt tgcaggatac agtgcttttt aaggacacca tagaggagaa
     7561 tatccgctac ggcagagaaa atgctaccct tgatgaaata aaggcggcgg caaagtttgc
     7621 gaacgcagac gagttcatag aaagactttc cgagggttac gacacagtgc ttgccgaggg
     7681 cggctcaaac ctttcacaag gtcagcgtca gcttctctcc atagcaagag cagtgcttgc
     7741 cgacccgaag atacttattc ttgacgaagc cacttcctcg gttgatacca gaactgagat
     7801 gcacattcag tccgcaatgg tggctcttat gaaaaacaga acgtccctta tcatcgctca
     7861 ccgactttcc actatccgtg acgccgataa gatagtcgtt atcgacggtg gacgtgttgc
     7921 agagcagggt agccatgagg agcttatcgc cgccaagggc tgttactaca gactttatca
     7981 gacgcagttc tcaggcgtta agacctgata caatggtgtt tggaaaacgt atatagccaa
     8041 tatcgtacat tcaaacaaat atgtagtgga gcgacgcaat ttctgctttg cataaatacg
     8101 acgtcccact tcttgatatt tcgtcaaccc ataaaacttc gtgataattc aaacttttac
     8161 actctcaaaa tcgggctgtc gagggcacta gcccctacat gaaaattggc ggtcttatca
     8221 aacaaatcaa ggcacaggaa aacctctcct gtgccttttt tatgcgcaaa aaagacggct
     8281 ctcataagaa agccgtcctt aatgttatct gaaatattac ttagaaataa gtgccagagt
     8341 atctcttgca atgagaagct cttcgtttgt tgggataacg taaacctttg tcttagaatc
     8401 gtctgtggag atctccttga gtattccacg gcagaggttc ttctcagcgt caatgtggat
     8461 accgaggata tcgagatttg aacaagcagc agctcttacc tcaggtgcgt tctcaccgat
     8521 accgcctgtg aagatgacag cgtcaaggcc gttcattgca gcagcgtatg agcctatgaa
     8581 cttcttgatc tgataagcaa atatctcgct tacgataaga gctctctcgt tgccctcgct
     8641 tgcagcagcc tgaatatctc tgttgtcaga gccgatacca gaaagaccaa ggaagcctga
     8701 cttcttgttc atgtattctg tcatctggtc aggtgtgaag cccattttct tctcaacata
     8761 gtcaacagca gaagggtcaa cagaaccgca gcgtgtgccc attacaagac cgtcaagagg
     8821 agtgaagccc attgatgtgt caacagactt gccgccgttg atagctgtga tagatgagcc
     8881 gttgccaagg tggcatgaaa ctatcttaag ctcctcaata ggcttgccca ttactcttgc
     8941 aagctcgcct gaaacaaatc tgtgtgatgt tccgtggaag ccgtattttc ttacatgaag
     9001 atcagtgtag cactcgtatg gcagaccgta gagataagcc tttcttggca ttgttgagtg
     9061 gaaagcagtg tcgaatacaa ctacctgtgg tgtgcctgct gggataactg ccttgcaggc
     9121 tctcagtgca agagcgtgtg ggtggttatg tacaggtgca agctcagcca gtgcgtcgat
     9181 ctggtcgata acttcgtctg ttacaaggca ggtctccttg aatacctcag caccctgaac
     9241 tattctgtga ccaacggctg atatctcaga catagagtcg atgacctttg tttcgcctga
     9301 tgtgagtacc tcaacgagct tttcaaaagc ctcagtgtga gtagggaaat tgcaatcctc
     9361 ctcgatgtgt ctgccgtcgc ctgtctttgc gctcacatgg ccgtcgatac cgatacggtc
     9421 gcagttgccc tttgcgataa cagactcgtc cttcatatcc agaagctgat atttaagtga
     9481 tgatgaacct gcgtttacaa ctaagatttt cattggaaaa aatctcctcc cgattataat
     9541 atgtcagata acgggcttga ctttaagccc ataatatcat atacttatag tatatcaata
     9601 ttaatgataa atttcaagag cttttttcat aaatatacat attgcacaat ttttttacta
     9661 gggcttaaca tattagtatc ataattcgca gtaagctttt gaaagacgga ataaaatatg
     9721 catagggtag aaagaggagt aaaaaatgaa aattgcggga atagttgcgg aatataatcc
     9781 gttccataac ggacacaaat atcagataca agccacaaga caggctggcg cagagggtat
     9841 cgtcgcagta atgagcggcg attgcgtgca gcgtggctca gcggctgttt tctcaaaata
     9901 tgacagagcg caggctgcta taagaaacgg tgcagacctt gttatagaac tgccatgccc
     9961 gttctcatgt tcaaacagtg aggtctttgc aagaagtgca gtaaggcttc ttgcaggtct
    10021 cggggaggac gtggtaacga ccttgtcttt cggctgtgaa agcggtgaca ggaacgccct
    10081 tgaacaggca gctgaaatat ccgcaatgct tgaaaattca cagcaggtaa gagaactttt
    10141 atctcaggga aagtcctacc cgcaggctat gtatgaagcc tccataggtc tttatggcag
    10201 aaccgccgaa gagatattct ccacaccaaa taacgttctt gcagtggagt atataaaggc
    10261 ggcaaagcgt atagccccat ggctagtgcc ttatgccgta aagcgtgaag ctgttgcaca
    10321 tgattctcag gcagagtctg gtgattttgc ttcggcttct catataagag agcttataaa
    10381 agagggcggt gagtggcaga aatttgttcc atatgatttt gagggctgta agccgtcctt
    10441 tacccggcag gcaggcagag aaatgctttt caagctctgc acagcttcaa gagaagattt
    10501 tctagcccta cctgactgcg acgaacacct tgccaacaga tttatgtctg tcgtaagctc
    10561 ctgccctgaa actatggata tctttgaaca gcgtataaag tcaaagaata tcaccatggc
    10621 aagagtaaga agaatggtcc tgcaccttgt tctcggcatc agaaaggacg atatcagaca
    10681 agtgccatac ggcaggatac ttgcctttaa ccgcacaggc acacgcatac ttgcgcaggc
    10741 tgagaacaga actcttgagt atgatacctc gctgaaaaag cttgaggata tctccgacta
    10801 tgcaaagcgt gttgctttcc ttgaaaagaa cgcagtaaga cttagagaaa cagcggcata
    10861 cggaagatgt atttcaaacg agtatacaag aaagataacc ataacctgag ataaacagga
    10921 gggcggaaca tgagaagaat gaaaggtgcg gcagggcttt tgacagcttt gctcttggct
    10981 tttcagcttt cggcgcagac ttttgcactt gccagcgagg ataacggcat aaaagtcgtt
    11041 cccgtaaact ttacccataa cggctatagc tatgagatag agggcgtggc caaaagcaca
    11101 gacggcgact atcagataaa gtcccctgca agactagccg cccgaaagct cccatccacc
    11161 tatgacccaa gaagctcagg agagatcaca acagtaaaag atcaggcttc aacaggtgct
    11221 tgctgggcat tctcagccct tgcctgtgca gagcaggatc ttatcaaaaa ggggcttgaa
    11281 aacccctccg cagaattttc cgtgcctgcc ttggtgctgt cctcaaattc aggcacagcc
    11341 acaggtaaag acgatttttc atcaggggga aactggctct ttgcggcctc cgcccttgca
    11401 aatggtcagg gactatgcta tgaggattac gagccgtttt tagaatccgg cacagagaat
    11461 atgatagttt ccgaaaataa gaaaaacgtc agcgaataca ggctgaatta tgtcatggag
    11521 ctttcaagca ccacgcaggt gaagcgtaaa ataatggagc ttggagcagt ttccgcaagc
    11581 tattttgcag gaaatggcta tatgaatcat aacaatactg cctactatga tcccgacgcc
    11641 tcaaaaaaca ctatcataaa tcattccgtc acagtggtgg gctgggacga taactacagt
    11701 aaggataatt tcagatataa gcctgccaat aacggcgcat ggcttgtgaa aggctcgtgg
    11761 ggcgcagatc aggacaatga cggcttctat tgggtgtcct atgacgaagc agagttcgga
    11821 cagttttgct gctatgactt tgaagaaagc tgtgataata catatcacta cagcaaaatg
    11881 acaggctatg ttgtgaacgc cagtaacgac ggctctgttt acggtgcaaa tgttttcacc
    11941 gctaaagccg atgaaaagct tgataaagca ggctttatgt atgtgggcaa aacgggcagt
    12001 gcggattaca ccctaagcgt ctataccaac gtttccgaca gcgaccctat aggcgtcctt
    12061 gaaacgcaga taagcggctc agtgtccgca aacggctttt atacagttga ctttcccgag
    12121 gatatccttc ttgaagaggg cgagaaatat tccatttcag taaaattcag cggcgacagc
    12181 ggcaggggat atctccttgc agaaagcgac cgaacatcaa aggcgcagtc agggcaaagc
    12241 tatgtttctc ttaacggcaa atattggtct gacgttggag cagataagac cgactccata
    12301 ggaagccttt ttatctatgc ttatactgac gatactgcaa agcccgataa aagttcactt
    12361 ggaaccaccc ttgcgaagta cggcaccctt gcaggctgtg aaagggaagc aaacaatgcc
    12421 cgaaaggtgc ttgcagatga aactgcttct aaaaatgata tctcaaacgc acaaaagctc
    12481 cttatctccg ctgcagaaga acaggacgaa actcttgtta taaccacaga agcccagtgg
    12541 gaaagctttg caaagcgtgt cagcggtggc gaaagcttcg agggcaagct agtcaccctt
    12601 gaaggcgacc ttgactttga tggaaaaact atctcacccg taggcaattc tgaaaaccca
    12661 ttcatgggct attttgacgg caacggacac cttataagaa acgcagttat ctcctcaggg
    12721 gagtattcag ggcttttcgc atacataaaa aatggtgcag agataaataa catttctctg
    12781 aaaaactgta tagtgaaagg cgattacgca ggtggaatag tgggttttta tcaaggaaca
    12841 gccataaaag cctgcacctt tgacggcaca gtgtcaggag aagtctattc aggtggcatc
    12901 ataggaagac aatcctgcgg tatcataaca gagtgttctt caaacctaag ggaaaacagc
    12961 tccgccatta caaatgcctt tataggaggc agggatattg ccgtttccgt tgtgaacgca
    13021 tacggctgtt acagcaatga cagcgacagc ctggtttcaa gcctttctgc aaaaaatgca
    13081 ctttctcaag gggcatatgc aatgaatacc tacggtgaaa agttcaaaga cagcgcaaag
    13141 tggacaatgg acggcactct tgtgcgccag acctcatata gcgagcaggc ttcccataag
    13201 ataacattct ccgctgtggc acagaccata gaagtctgca ccgattatgc aggcaaagcc
    13261 gtgttcccga atgtgaacgt tcagcagggc ttcaccctcg gctggtatcg taaaggtgaa
    13321 cctgtgaaca gcaacacagt tttcacagaa aacaccacag tttccgcaaa aatcactcca
    13381 agtgacaaag caaagataga ttatatcctg aacggtggcg aaaacagctc cctcaaccct
    13441 tatgagcttg gcgagggcga aaccgcacag ctttccgccc ccacaaaaga gggcgcacag
    13501 tttacaggct ggtatgccga ctcacagctt gagagcgagc ctataacgtc cgtgagcttt
    13561 tcagacggct caaagacgct ttatgcaggc tggaaagcca atacctgcaa ggtcgatttc
    13621 accgatataa acggcacagt tttgtcatct cagaccgtag aatacggcaa aagtgccgat
    13681 ccgcccaaag caccgaccat aaaaggcaaa cgctttacag gctgggataa agacttttca
    13741 aaagtcacag cccatatgac agtgcaggcg gtctataccg acagaaagct tataaaagac
    13801 tgcgagatat caggctttaa aacacatatg acattcacag gctatgagct tgctcaaagc
    13861 agtataacgc tttcctatgg cgataccact ttgacaaata ataaagacta taccattgac
    13921 tatgccgata atatagccgc aggcaagggc aaaatgataa taacaggtat tggcggatac
    13981 agcggcgcta tagccaaagc attcgacatt cttccaaagt ccgcccaatt gacaagcgtc
    14041 tattatgttg atgcgctctc ctacacaggc aagcctataa gacctgacgt tatggtaacg
    14101 gacggaggaa aatttcttct tcctgaacat gattatactg tttcctatag ctcaaataca
    14161 aaaataggcg ttggaaaaat aatcataagc ttcatgggca actatcaggg tactataaca
    14221 aaaacattta ccatttgtcc tgcaaaacag aagatacaat ctctgcaaac aagatataaa
    14281 ggctttttta tcgactttgc tcagaaaggt tcagccacag gatatgaatt gcagtacagt
    14341 acacgctctg acttcgcagg tgcaaaaacc ttgaatatat cctcaaacaa aacggataaa
    14401 aagacagttt caaacctgac ggcagggaaa aaatattatg taagagtgcg ttcgtataca
    14461 atagtcggcg gcaagcgtta taacggcgca tggtctgata caaagagcgt gaccaccgca
    14521 aaatatgata ttgccaaagc taaggtagca ggcataaaaa acaagtcttt cacaggcaag
    14581 aacatcaccc aaagcataac agtcacatat tcgggcaaga cccttaaaaa cggcacagac
    14641 tatatagtga aatattcctc caataagaat ataggcacag ctaaggtagc agtaacagga
    14701 aaaggctcgt atggcggagt gataacgaag tcttttgtca tcacccctgc aaggcagacc
    14761 atacaaaagc ttatgccagt gaaaaaaggc ttttatatag actatgcaca gaaaggctct
    14821 gctacaggct atgagatatc ctatgctgaa aatatatctt tcagcggtgc caagacagtt
    14881 aaggtgacct caaataagac cgacaaaaag accgtaacag atcttaaaag ccaaaagaca
    14941 tatttcgtcc gtgtgagaag ctatacagcc acaggggaca agacctattt cggacagtgg
    15001 tctgatatag ccaaagttta taccaaataa ttatccatgt tatcgggagg gatagcaatg
    15061 atcacaaaaa caaagcttgt aaaaagaata ttctcatcgc tcttgacagc cgcacttgca
    15121 gttccaatga cggctctcac tgcccatgca acaggggagg ctttcttcta tccgcctaaa
    15181 aaccccgtta tgaccgatca gcagtttgaa gtttccgtga cattttccgc agattcagag
    15241 ataggaagcg ttcaggcaaa tatcgcctat gacgattccg ttatagaatt tgtgtcaagc
    15301 gacaatgcct caggcggtgg aggagttctt acagtaaacg gatttcccga taccccctct
    15361 tcagaagtgg atttcagctt tacgttcaaa ggtctgtcgg caggaaatgc aaatataagc
    15421 ctgcaaagct gtcagatata ttcccctgat tcagagctta tcggctcgcc taccgcctat
    15481 tgtgtcataa cagttggagg cgataaggtg gaaaccaaga ccgaaacaga aaccacgacc
    15541 accaccgaaa aaaccaccac taccaccact gccactaaga ccgcaccttc aaacggcata
    15601 cccgataaag gcgttcttac agccctgaca gtggataacg gcgagctttc tcctgccttt
    15661 gcctataatg tctataacta tacagtgaac gttgacaatt ccgttaccaa tgttgagata
    15721 gagggaacaa cagcaagccc ctctgattac atatggtata caggcacaag cgagtgtcag
    15781 gtgggcgaaa atgtacgaac cataaccgtc acagacgttg acggcaatgc cgccacctat
    15841 acggtcacca taataagagc cgcaggcgga gatgaacagc agacccagac cgtccaaaag
    15901 aaagacagct ccgatacttc atctgccgtg tcaaaaaacg caaatgttat ggaccagtat
    15961 aaaaagatgc tcaataccgc ccttgctatc gtccttattg tccttgttat tgcacttgtc
    16021 gtaataatta tctggataag aggaaagatc aaaggcggca aaaaggataa ataagttctg
    16081 tgtaaagaga tgataaacag cc
//
LOCUS       DAWEMR010000043        16090 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1511_length_16090_cov_8.763205, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000043 DAWEMR010000000
VERSION     DAWEMR010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16090)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16090)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16090
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1511_length_16090_cov_8.763205"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7285..7344
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3944 a         3868 c         2953 g         5265 t
ORIGIN      
        1 ttcttacgct ttaccgaagg cttttcgtag tgttctcttt tacgaacctc tgcgataacg
       61 ccgtctcttg cacatgatct cttgaaacgc ttaagtgctg tatccaaagt ttcgttcttt
      121 ccaacacgga tttctgccaa aattattccc tccctttgcc acctgtgaca gaggcaattc
      181 tatcggaatt tccgaaaatc aacggtgata gtgaaggtgt ctgcaaaagc aaactgaaac
      241 gctttacgtt cccccagtcc tactctttcg gaccgctctg tctttaatct gcggaattta
      301 taaaaccgat ataattacaa atttaattat actatatttc caccaaaaag tcaatagctt
      361 tctggctctt tttttgaatt ttctgaggaa attttcgctt actttacaac cagatttaca
      421 agcttgttag gaacatagat ttccttgact atacttttgc cttccgttgc cttaacgata
      481 gcttcgtcag ccttagcaag tgcgataacg tcgtccttag ctgcgtctgt aggtattgtg
      541 agcttggatt ttaccttgcc gtttatctga caaacgatct cgatagtatc gtccttgcag
      601 agtgcttcat catatgttac ccactcctgc tcgtcaagaa ctccctcgca accgataatc
      661 tgataaagtt cttctgtaac atgaggtgca aaaggattga gtatgataag aagatctctg
      721 aactctttct ttgtgataga gccaacatta tatatctcat taagaagcgt catcattgcg
      781 gcaatagcag tattgaactt cattgactcg atatcatcgg ataccttctt gatagtcttg
      841 tgcatgagcg atctcagctt atcgctgtac tcgtcgctgt caactacctt atcctgcaaa
      901 gcccaaattc tgtccacaaa tctcttacag cccttaacac tgctctcgct ccatggagct
      961 gccttttcat agtcacccat gaacagaaca taaagtctga gaacatctgc accgtattcg
     1021 tcaacaacat cgttagggtc aacaacgttt cctcttgact tactcatctt ctcaccgtca
     1081 ggaccaagga taagaccctg agctgttctt tttgcataag gctctgaagt tggtacaaga
     1141 ccgagatcgt agaggaactt atgccagaaa cgtgaataga tcatatgacg tgtaacgtgc
     1201 tccataccac cgttatacca atcaacaggc atccaatatt tcagtgcttc ctgtgaagca
     1261 aactcatcat tgttgttagg atcgcaataa cgcaggaaat accatgaaga accagcccac
     1321 tgtggcattg tatctgtttc tctctttgcg tcacagccac acttaggaca tttgcagtgt
     1381 acgaaagagt ctatcttagc cagagggctt tcgccgtctg taccaggctc aaagttctct
     1441 accggtggca atttaagtgg aagctcatca tatggcacag cgaccattcc gcagtgaggg
     1501 caatgtacta tcgggatagg ctcgccccaa tatctctgac gattgaaagc ccagtctttc
     1561 atcttatact gaacgccctt ttcaccgcag cccagttttt caagaacagt aagcatcttt
     1621 tcgatagctt cttttttgtt tgttatgccg ttaagctcac ctgagttgac catttcgccg
     1681 tcgcctgtat aggcttcctt agaaatgtcg ccgcccttga tgacttcgat tatgtcaatg
     1741 ccgaacttct ttgcaaaatc atagtccctt gtatcgtgag caggcacagc catgatagca
     1801 cctgtgccgt atcccatcat aacataatct gagatgtaga taggtatctc tttgcctgtg
     1861 ataggattga tagctgtcag accgtctatc ttaacgcctg ttttatcctt aacgagctga
     1921 gttctctcaa attcgctctt ctttgcacac tctgtcttat aagcgtcaag gtcagcaatg
     1981 tttgcaatac tatttctgta tttctggatg ataggatgtt caggagcgat taccatgaat
     2041 gtaacgccgt aaagtgtgtc aggacgtgta gtgtagatct tgagctgctc gtcctgctct
     2101 ttgatcttga agtttacaaa tgcacctgtt gattttccta tccaattctg ctgctggagt
     2161 ttgatgttag gcagatagtc aacttcttca agtccctgaa gaagcttgtc agcgtattct
     2221 gtgatcctaa gataccatac ttctttagtt ttctgaacta tctcgctgtg gcaaacatcg
     2281 cagtgaccgc cctgtgaatc ctcatttgaa agaacgacct tacagcttgg gcaataattt
     2341 acgagggtct tatctctgaa tacaagacca ttctcaaaca ttttaaggaa tatccactgt
     2401 gtccacttat agtagttttc atcggtcgta tcaataactc ttgaccaatc gaatgaaaaa
     2461 cctactcttt tgagctgccc cgtgaacttc ttgatattgt cgtctgttac ctttcttggg
     2521 tgaacacctg ttttgatagc tgtgttctct gtaggcagac cgtaagcgtc aaagcctata
     2581 gggaaaagaa cattatagcc ctgcatacgt cttttacggc ttacaacttc tagaccggaa
     2641 tatgccttga tatgtccaac gtgcataccg tgacctgacg gataaggaaa ctctacaagt
     2701 ccgtaaaatt tcttcttcga aaagtcattg cttgcctcaa aggtcttatg ctcgtcccag
     2761 tatttctgcc atttgctttc tatggccgaa aaattgtatt ctttcatctt tatcaatcct
     2821 ttgttaaatt ataattgtca aaattaatcc tgtggctcgt ctggtaagaa ctgctttacg
     2881 tcgatatcct tgccgtcaga ccatagtctt tcaagattgt aatgatctct tgtctcctta
     2941 tagaaaacat gaactatgat atcaccgtaa tcaagtacga cccaagttga accctgatag
     3001 ccctctgttc tgttaggttc aatgccaagc tggctcattt tgaactcaac ttcctccgca
     3061 agggcctttg tctgagtgct tgaagtacca tttgctataa caaaataatc cgcaactata
     3121 gtcaggtcac cgatctctat tatcctgatg tcctctgctc tttttgaatc aagagcttta
     3181 acaatagttg acagcttttc attctgtgaa atattatcca taaaaatctc ctttattttg
     3241 aattatcggc aaaatcatta tatgcttcaa gtgtggaaac agggatagta ttaccctttt
     3301 ccactgagtc ttttattgag aatttcaaag cctcaaacat tgctttctcc aagctttgtt
     3361 cggcatactt tctcattttc tttacgtctt tataatccct gtccgcagaa ataaggtctg
     3421 caagatatac tatctggcta agcttgggca tattgccaca ggcaactgta tgatagcgta
     3481 tagctgagat tatctcagga tcatggatat caaaaagcgt ctgcacaagc tctgcccctg
     3541 caataccgtg aaacagcgga acagcctttt tctctatatc gcacacatta agtgaagatt
     3601 tagaaactat agccgcaagc tccaatgctg gtatctcttt tgcaacatca tgcaaaagtc
     3661 ctgcaacata agccttgtct gcgtcagcac catatctttt tgcaagctcc acagcggaat
     3721 ttgccacgtt cacagagtgt gtgtatctct tttttgataa atgttctttt aggtatgctt
     3781 tataaatgcc tattgttttt ttatctgcca cttagaaaac ctcggttata caaatttttg
     3841 tccactatat attgtacaac attttcactc aaatagcaag agcaatcaac attttttttg
     3901 agcatttgac gtatctcagt tgaagacacc tcaaatgggt cggtttttat tatgattatc
     3961 tctccaccct cggctttgag ctgtttggct ttcacctcta tgtggtcaat gtcctcatgc
     4021 gagcgtgcaa cgcagataag tgccgaaagt gataatatct ccttatactg ataccattct
     4081 tcaaatgaaa gcaacatatc gctgcccatt ataaaaaaca gcttatcatg tggatatatc
     4141 tcttttagat gtctgagtgt tatcactgaa tagcttttgc catcttgctt aagttcccaa
     4201 tcggatattt ccacacatgg gatatctgca aaagcaagtc tgcacatatt caacctatcc
     4261 tcggaggaag ctaagtcctc cgcttgctta tgtggaggta ttctgtcagg catgacgatc
     4321 atcttgtcaa ggcccgcctg ctccgctacg cttttaaggc aatttaaatg ccctaagtga
     4381 acaggattga acgtgccgcc gaaaacacct attttcatta ctttgacttt aattttataa
     4441 caggctgctt ctcattgcgt ttataaagta ccgcctttga gcctattacc tgaacgactt
     4501 cagcagatat cttttcagcg atctcctctg cggcttccct tgcggtgtac attgagttgt
     4561 caagcacctt tatttttaca agctcgtgaa ctctcagata atcgtctatc tgtgccacct
     4621 gagcgtcatt aactccgccc ttgcctatct gaaaagcagg ctgtataagc gaagccatac
     4681 ctttgagttc tgctctttgt ttagttgtaa tcataatcta aatcctttca gtttactgct
     4741 ttgtgagctg ttcaagcaaa gctttcagtg cgtttgctct atgggaaacg gtattctttg
     4801 tttctgagtc cacctgagca aaactcttgt tcccatacat gaaaataggg tcatagccga
     4861 agccgttttc gccaacaggc tcgtgagcta tcttaccgaa tacttttccc tcaacggcta
     4921 tctcttcacc gtctgctttg ataaagtgta ttgtacaggt aaagtgtgct ccccttttat
     4981 catcaggtat ttctttcatt tttttaagca caagagcggt acgctcatca tcagtttcga
     5041 gtccgccata tcttgctgag tatactccag gctcaccatc cagggcgtct atctcaagac
     5101 cgctgtcgtc tgcaagtaca gcacagccgc aaagcttgaa tgcagccctt gcttttagtg
     5161 cagagttttc agcaaaagta gtgcctgttt cctcaacatc aagtatcaga cctgcctctg
     5221 attgcgagaa cacctcataa ccaagcggtt caagcagctg tttatactct cttattttac
     5281 ctttattgtt gcttgcaata acaagtttca tgttttctcc tttagctgtg tatataagtt
     5341 accttatttt tctttaattt ttcacggcat ttgtttgcct tatctttgaa aactgtaccg
     5401 atacattctt cgttgaaaac aactttcatt ccacattcaa tagcacctat tgcagtgttg
     5461 ataacggaga taccaccatc aacacctaca aattctatct ctttcacatt ccttgctctc
     5521 ataaaatctt caagagcaac gtttgaaaaa gcatcgggct tgcttttgga ataaatattt
     5581 ttggatacaa ctttcagctt tgctggaaag ctagccgcag gtgttccctc tttgggcatt
     5641 cctccaccca gaagcccctt tttgtagctg acgatataaa agacctcctc agctttataa
     5701 gcagagatac gcttgtttat ggcgtcgata agagattcgg catcatatga gtatgtggtt
     5761 ttatctctgc cccttcctac atacatttcc tgcatatcaa ctacaaacaa aaccttatcc
     5821 ataaaaaaat tccccctgat tttatacttt taagtgtcta atccaatatt ctattattca
     5881 aacagtgctt caacaaaatc tcttgctgaa aacggctgta tgtcgtctat tttttcgcct
     5941 actgtaacaa gctttacagg cacttgcaga tcgtcggtta tgctgataat tattccaccc
     6001 ttggctgtgc cgtcaagctt agtaagaata atacccgtta tatcagctac ttcgttgaac
     6061 tgtcttgcct gattaactgc gttctgcccc gttgtagcgt caagcgcaag caggacttcg
     6121 agagcgcagc cctctgcctg ttggtgaact atacgggata tctttttcag ctcgtccata
     6181 aggtttttct tattgtgaag acgacctgcc gtatcgcata taacaacgtc agcctgtctt
     6241 gccttagccg ccgccagtgc gtcaaatacc acggaagcag ggtctgaacc ttcgttgtgc
     6301 ttgattatgt ctacccctgc tcgctctgtc cacacttcga gctggtcaat agcagccgct
     6361 ctgaaagtat cagccgccgc aacgataacc tttttgcctt gtttgtgaag ctgataagca
     6421 agcttgccga tagttgttgt tttgccaaca ccgtttacac cgataaccag tataactgac
     6481 ggtgttgttg acatatcgag cttctgatcc tcaccgagca tttctgtgat tatctcttta
     6541 agcatatcct tgattttttc aggctcttta acgccctcct gcttgacttt ctgtctgagc
     6601 ttatcgcata tcttttcaga agttgtaaca ccgatatcgc agaggataag cgtttcttca
     6661 agctcctcaa aaagatcttc atctattttg ctgaatgaat ggagcagact ttctattctg
     6721 cccatcattg agtctttagt ctttttaaga ccatttttca gcttttcaaa aaatcccatt
     6781 tcttcactcc tattcaagtt tcatttgacg tgtttccgaa acgtccattt taagaagctt
     6841 ggaaacaccc ttttcttgca tcgttacgcc atacagaacg tcagcttctt ccattgttcc
     6901 acgtctgtga gtaatggtga taaactgtgt tttatctgta aggtggtgca gatattgtgc
     6961 atatttactt acgttcacat catcaagcgc cgcttctatc tcatcgagaa gacagaaagg
     7021 tgatggcgaa attttcagga tcgcaaaata aattgcaata gccaccagag cctgctctcc
     7081 gcctgaaagg gacataaggt ttgttatgac cttgcctggc ggctgaacgt ttatctcaat
     7141 accacattca aggacattgt ttgggtctgt gagaataagc tcgcactgac caccaccgaa
     7201 aagctcggtg aaaattttct tgaagttcgc atttatgcgc tcaaagctgt cggtgaacat
     7261 attcttcata ttttctgtca gatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnaaattc gtgtcgttgt gacacttctg catattcctc
     7381 tatagctcca aggtttacgc ttccaagatt tcttatcttt ccacgaagtt cgttgagtcg
     7441 cctgttagct accgttatat ccacaatatc ctcggctatc tctgcagcgt cgcttcttgt
     7501 aagctcataa tcgtcccaaa gctgtgccac aagcttatca aagcctgcag taacagattt
     7561 tttctgttcc tccgctcttg taagctgacc tgaaagtgtt tccttctcgt ccattttgtg
     7621 cttttgcttg gtgcgaagtt tgtttgctgc attgtcaagc tcaatatgct cacgctgagc
     7681 ctgctccgcc attttctgtg cggcggctat cttttcgtca gcgccgctga gttcctgttt
     7741 ggtctgaaaa ataagctctt tcttctcggc tattttcttt tcacactcgg agatattatc
     7801 gttcaacttg ccgctgtcag tatcactgtc gccgatagtt tcttcaagct gtgatattga
     7861 aagcttgcag gcatcaaggt ctttggataa ctctgccgcc attatcttca tttgcgaaag
     7921 ctcttcgctg agctgttcac gcttattttt aagttctgcc tgctcgttct gagagtcggc
     7981 aagcttggct tcattatcag atatctcagc ttcgatttct gaaagttctg tctgagcctt
     8041 gtcgatatct ccgcttacta tcttgtattt atttttcagc gtttcgataa atgtttctat
     8101 ctcatcaagc ttgttttcat agtcagaata atactctgaa acacgcttta tttccatttc
     8161 aaaacggagt atatcgctgt ttacctgtga aagcttttcc cttagtccct cggtatcagc
     8221 ggtgtatttt gaaacctctt gatttagctt ctcaacctgc tgtttaagct ctgtaaagga
     8281 gttttcaagt gctgttttct ttttagcaag tgcttccgct tcattttttc ttgtaagcag
     8341 acctgtctgt ttggaaacgc ttcctcctgt gaatgagccg cccgcgttta tcacctgtcc
     8401 gtccagggta acgatgcgga atttatagcc gtatttcttt gcgataaggg ttgcactgtc
     8461 gatatcctct gctatacata tcctgcctaa aaggctgttg attacgccct caaactggct
     8521 gtcatactta acaagctcac agcctagagc cacaaagccg tcctcatttt caagttgcgg
     8581 catatttagc ctgtttcctc gaactgacgt aacaggcaga aatgttgcac ggcctgctct
     8641 gttctctttc agaagcctga tacctctctt agcagagtcc tcattgttta caacaatatt
     8701 ctgaagtgcc ccacccaacg cagtttcaat ggctacagag tattttgttt caacagatat
     8761 aagctgagaa acagtaccgc aaatgccgct tatcctaccc tgctgtgagg ctttcattat
     8821 gtgctttacg ctgaactgaa agccctccat ggagttttca aggtcggcta tagtctttat
     8881 cctgttctca acgtcacgga gctgtatttc cgtatctgtg agatttttgc gacattctga
     8941 aagctttgtg tttctagttt caagaagctt tgcaagtccg ctcatgcggt ttgtaagctc
     9001 ctgcttgttt tcgtcagctt tgccttttga ttcacgcagt tcctttaatt cctttgaaga
     9061 aagttttgaa tgttcttcaa gctgtttttt tctttcaaga gaattttcga tctgctcatc
     9121 aatatccgaa aggttgtttt tagcattttc aagtctgaat gaaacttccg actttttcat
     9181 gtaaagctca tttataaagc ttccagcttc acttaccgac ttgtcagata atgcagtttt
     9241 atcaataagc tcagaaagct ctttttcctt acactctaca gcttcctgct gctttgcttt
     9301 tttctcgtcg aattcttcaa gctcctgtct gcgtgttttc agctcagcct caagatagta
     9361 ttttgatgag ttggactcac ttatctgctc tttaagcttc aaaatattat cttcaaggtg
     9421 agaaatatca ttctcatata ctgcgatctt tgacagtgcc tgagagtttt caagctccac
     9481 ctgatgtatc tgatttttgt agctgtcagc aagctcagat ttttcagcgc tctttgcaaa
     9541 gctgtcattt atatcatctt caatgctttc aagctcctgc gtgatgacct cgtactcttt
     9601 ttttacagaa gaaagcttct cctcataacc tgctatttgg ctcatatact catcgagctt
     9661 atgcacccat acggaaactt ccagctttct cttttcttca tcaaggactc tgaacttctc
     9721 cgccttttca gcctgcgctt taagcggacc tattcgtcct tctatctctc ccatgatgtc
     9781 attgatacga gagagattat cctcagccgc cgcaagcttt ttttcggctt cttttttctt
     9841 tgaacggaat tttgaaatgc ctgccgcttc ttcaaatatc tcacggcggt cattggattt
     9901 tccgtttacg atatctgcta tcctgccctg accgattatc gaataaccgt ccctgccaag
     9961 accggtgtcc ataaacagtt ccacgatatc ctttaaattt acttctttac cgtttatcat
    10021 gtaaaagctg tcgccattgc ggtaaagctt tcgtgatact gacacggtgt cgctgtcata
    10081 ttcaagtgca gcgtcctcgt tgtctatggt gatagttacc tgagcaaaag gtgcttttgg
    10141 tctagtttga gtgccgtgaa aaataacgcc tgccattttt tcacctctca gggctttgga
    10201 agattgctcg cccattaccc agcgcatagc gtcaccgata ttactcttgc ctgagccgtt
    10261 aggaccgaca actgcggtga tacccttatt gaatttcagc tctattttat ctggaaaaga
    10321 cttaaagccc tgcaattcaa gtgatttaag atacattatt tttcaccacc cctcattatg
    10381 atttcgttta tctttatttt atcgctgcct tcgtgaccta taatttttat attgacacca
    10441 agatctttga aatattctat attagctttt ttctgaccta ttgccttgct tatctgcctt
    10501 tttggtacag atatcacaac ggaagctata tcgcttatat taccatgctt tccaagctcc
    10561 tctgctataa gcctgcggta atttctgcct tcgcaaagtt cacggaatgc agggtgataa
    10621 aatccgccca acatttgctc ctctacgacc tcgcttgcgt gcagaccaag ctttataaca
    10681 tctataccaa gtttatcggc ttctgtgaga agataagaac agattttcac tacctcgtca
    10741 aagtccatag gcttatattc gccacttttg taaagctctc cgagccttgt accattaagg
    10801 ataacaacgg gataaatacg tactgtttta ggagacagcg ccgctatctt cgaccaagtt
    10861 tccagctcag tgtcacggtt gcttttataa agtcctatca tcatttgcag accaaggtca
    10921 aagcagccat agctttttat aagctttgca gcgttttcaa cgtcctgaac agtatgtcca
    10981 cgctcgttgg cttcaagaac aacatctgac atagactgtg cgccaagctc gatagccgta
    11041 acgtgattat ctctgagaat gtcaagtatc tcagtgctga tatagtcagg tctggtggat
    11101 atcctgatac ccttgaattt gtcgttgcct ataaactcct gtgctgcacc aagaagttcg
    11161 agcatatatt ctctcggcac tgctgtaaag ctaccgccga aaaaggctat ctccgtctgt
    11221 gaaacgtcct tgacttcgtt aattgcctgt gtgcagatac gcttaacatc atcagcgtgc
    11281 ggtatcttct ctgtaccagt tatggtgcgc tgattgcaga acgcacacat atgcggacag
    11341 cctatatgcg gcacgaagac agatatatta ctgtgcccca tagcccatta gttccaatgc
    11401 ttctttagcc gccaactgtt cagcggtctt tttcgatctg cctgtaccct cgccgataac
    11461 attcgtattc aaaagcacct ggacagtaaa tttcttatta tggtcaggac ctgactcgcc
    11521 tttaaggtga tattctattc tctcctcagg atttttctgg attatctcct gaagaatagt
    11581 cttgtaatca tggaaaacaa ctccatcagg cgtgatattt tcaggtatga atgaaagtat
    11641 atattttgaa acaggctcga taccaccgtc aagataaatt gcagcgataa ctgcttcaaa
    11701 agcgtctgaa ataattgacg gtctttctct gccccctgtt atctcctcgc cttttccgag
    11761 aaagatatac ttgccaagtt ctattttctt tgagaactca aacaatgctt tttcgcacac
    11821 aagtgaagct ctcatttttg tcagctcgcc ctcaggcata tgcttgtaat gctcgaaaat
    11881 gtgatctgac acaactatag aaagcactga atctccaaga aattcaagcc tttcattgct
    11941 gtttcttgcc ttcttgtttt catttgcaaa tgatgaatgg gaaagagctt cgtaaaggag
    12001 cttttcgtct ttgaactgat aacctattct ttcctgaaac tctttaagag ccttttgctc
    12061 gtccattata acatctcctg tttgcttttc aactgctcga tagcagttgt gatctcgtct
    12121 atcacattag tttcagtaaa ttgttttgct tgtctaatag cgttatagaa tgctcttgca
    12181 tttgatgagc catgtgcctt gataacaggc tttgcagtac caagtagagg cgctccgcca
    12241 tactcagagt agtctacctt tttcttgaat tctttaacgt tcttcattac cataagagca
    12301 gcgatctttg actttgtatc cttagtcaga agcgttttaa gctcccctga gaagaatttt
    12361 gccatgccct cgtaaagctt gagcataagg tttccgcaga agccgtcagc cacgcatacg
    12421 tcacaatcgc ccataggaag ctgtcttgct tcgatatttc caacaaagtt tacaggtgcg
    12481 tttttcaact gcttgtaagt ttcaagctcc agctctctgc cctttgactc ctctgcacca
    12541 atatttgcaa ggcctactct tggtgactca acaccaagta tcttgttcat ataagccgaa
    12601 cccattattc caaactgagt aagcatttca gggcggcaat ctgcgtttgc accgctgtca
    12661 agaagcatcg taggcgtcgt tgccgtagga agtattgttg caagagctgg tcttttgatg
    12721 ccctttattc tctttacgat aaacgttgct ccaactacaa gtgcgcctgt tgagccagct
    12781 gaaacgaaag cgtcgccctc gccgtctgca agcattttca tgcccacagc cattgaacaa
    12841 tccttcttgc ccttgattac ttctgtaggc tcttcacata tctcgataac agtatcagcg
    12901 tgcttgatat gtaaagctgt gatatcaagt ccgttttcct gtgcacattt ttttattttt
    12961 tcttcatcac caacaagagt aacgtcaact ccgaaatctt tctgtgcgtc aattgaacct
    13021 tttatgactt cgagaggtgc gttatctccg ccgaaagcgt caattactat gttcataaca
    13081 ttctccttaa aattcctcaa tagccttgtc aaagtctgca agaccccttt cagctatcat
    13141 cttatatttc aatttcttat ctcttatacg ttcaggcagc tcaggcagga tacccatatt
    13201 acatcccata ggctggaact tctccacagt aggatcgctt atatactttg aaagcgcacc
    13261 cagcattgtg gtatcaggca aggtaaccgc aggcttgccc tgcaaacgtc ttgccatgct
    13321 taaccctgca aaaattccgc ttgccgccga ttcgatatat ccctcaacgc ctgtcatttg
    13381 acctgcaaaa taaaggtttt ctctatctcg catattaaac tgcggattta agagcttagg
    13441 cgaattgatg aaagtgtttc tatgcatgac tccatagcgt ataaattctg cattttcaag
    13501 tcctgtgata agagaaaata ctcttttctg ctcaccaaat ttaaggtttg tctgaaagcc
    13561 cacaaggtta tacagacttc cctctgcgtt ctctgcacga agctgaacga cggcataagg
    13621 tctgtttcct gttcgtgggt ctgtcaaacc tacaggtttc agcggaccga aacgcattgt
    13681 gtcctctcct cttttggcaa ggacctcaac cggcatacag ccttcataca ccttgaaccc
    13741 gtccttagaa aaatgctcct tatcaaaatc atggagcgga gcggattccg cattgataag
    13801 ttcattatag aacctgagat attcttcctt attcataggg cagttgatat aatcagcttc
    13861 tcccttgccg tatctcgaag caaaaaacac cttatcctta tcaagacttt catatgtgac
    13921 gataggtgcg gcggcatcat gaaaatacag ataatcaccg catattttgc cgatactctc
    13981 tgcaagccca tcgcttgtaa gaggacctgt tgcgattata acatcgccat caggtatatc
    14041 tgtgacttct tcctcgataa cagtgatgtg aggattttcc attatctttg ccgtgaccat
    14101 atcggaaaac ttttccctgt caacagcaag tgctccgcct gctgatacct tagtttcagc
    14161 tgcacattcc attgtgacag agccaagcct gcgcatttcc tcttttagca tacctgcggc
    14221 tgaatctatt ctgtccgctt tcaatgaatt tgagcagaca agctctgcaa aacccttata
    14281 tttatgggca ggcgtaaact ttttcggctt catttcataa agctcaacgt ctatccctgc
    14341 ttgggctaac tgccacgcag cttcgcagcc cgcaagtcct gcacctacta ccttaaccgc
    14401 cattagataa attcctttca tatccgcaat caggcttatg gcaaatgagt attccgttct
    14461 ttccgccttt ctggaaaagc gtactgccac acttagggca tttctcttcg gtaggaacat
    14521 cccatgtcat gaacttgcac tcaggattgt cctcacagcc gtaatacttt ttgccctttt
    14581 tggacttttt caaaagcact ttctttccgc agtaagggca aattccgcct gtttcctgaa
    14641 caattttctt ggtattcttg cactcaggaa aacctgagca agccataaat ttaccatatc
    14701 tgcctatctt tattaccata ggtttgccgc aaagctcgca cacctgatct gtttcttcgt
    14761 caggcacttt gatacgcttc ccgtccatgg cttcctcggc ttcagtcaat tcctcgtcaa
    14821 aatcactata aaaatcctgc aaagttttta cccaactttt cttgccattt tccacttcgt
    14881 caagagattt ctccatgttt gcagtaaact taacatcaac tattttatca aaatgctcgc
    14941 tcataagctg gtttgttact tcgccaagcg aagtcggctt aagctgtttt ccgtcacgct
    15001 caacataatt cctgtttatt atggttgtaa tagttggaac ataagtcgaa ggtctgccga
    15061 tgcctgtttc ctcaaacgcc tttacaaggg tcgcttctgt gtatcttgca ggaggctgag
    15121 tgaaatgctg atttccctca aggttacgca cctttattga gtcaccggat tttattggag
    15181 gaagcacgtt tttcttttca ccctcatcgt ctttagcctc ttcatataga acagtaaatc
    15241 cgtcgaactt tacagaatag cctgttgcct tgaagttaca accatttgca gagatatcaa
    15301 cagaaacagt gtccataagg cagtttgaca tctgagaagc tataaatctc tcccaaatga
    15361 gtttataaag tttgtactgg tcactcgtca ctccgcttgc ctttaccata tcaggtgtta
    15421 cctctgggtg tgttggtcgg atggcttcgt gagcgtcctg agcgttgccc ttagttttat
    15481 attttttcgg tgtgtcaggg atatacttat cgccatattt ttttctgata aaatcatatg
    15541 ccgctgctct tgcttcatca gaaattctaa ggctgtcggt acgcatatag gtgataagac
    15601 ctgtctgacc catatcgttt atctcaacac cctcataaag ctcctgagcg gttttcatcg
    15661 ttcttctcgc ctgaaagctg agacgtcttg acgcttcctg ctgtaaagtc gaggtggtga
    15721 atggtgcggc aggagatttt ctgtgaacgc tcttttttac cttgtcgata acaaactcgg
    15781 cgccctcaag tcttttcagt atctcgtcag cctgctcttt atttttcaac tctggctttt
    15841 ctccgtcaac tgtattaagc tttgcggcaa aggtctttct ttcaccgttt gcagaaaatt
    15901 ttccgtcaat ggaccaatat tcctgagata caaatgccct tatctcgttt tctctgtcgc
    15961 atatcatttt aactgcgaca gactgaactc tgcccgctga aagacctttt cttatctttc
    16021 tccaaagaaa aggtgaaagc ttatagccca caagcctgtc aaggatacgt cttgcctgct
    16081 gtgcattaac
//
LOCUS       DAWEMR010000044        15929 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1530_length_15929_cov_11.490425,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000044 DAWEMR010000000
VERSION     DAWEMR010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15929)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15929)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15929
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1530_length_15929_cov_11.490425"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2517..2526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11758..11767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4614 a         3386 c         3727 g         4182 t
ORIGIN      
        1 tatcagggag aagtgcaacg atagtcttgc ccttgttctc aggtctcttt gaaagtatct
       61 cagcggcttt aagtgctgcg cctgatgaaa taccaacaag gataccctct gttttagcga
      121 acttgttgcc tgtcttgaaa gcttcctcat tctcaatagg gaggatctcg tcataaacct
      181 ttgtatcgag gacatcagga acgaagcctg cgccgatacc ctgaattttg tgtggacctg
      241 atttgccctg tgaaagaaca ggtgaagttg caggctctac tgctactatc ttaacgtcag
      301 ggttctttga tttgagatac tgaccaacgc ctgttactgt tccgcctgtg ccaacgcctg
      361 cgataaagat atctacctta ccgtctgtat cgtcccagat ctcaggacct gttgttgcaa
      421 agtgagcggc tgggtttgct gggtttacga actgaccaag gataactgag ttctcgatct
      481 cgcccttaag ctcgtcagcc ttcttgatag cacctgtcat gccctttgca ccttctgtga
      541 gaactatctg tgcgccatat gccttaagaa gatttcttct ttcgatactc attgtttcag
      601 gcattgtaag aattaccttg tagccctttg atacgcccac agccgcaaga ccgatacctg
      661 tgtttcctga tgtaggctct ataatagttg cgcctttctt gagaacgccc ttcttctcag
      721 cttcctcgat cattgcaagt gcaactctgt ccttaacgct tcctgccggg ttgaaatatt
      781 caagctttgc aagaatagtt gcgtcagcct ctactgcctt ttcaaaatta actgcttcga
      841 ttattggagt ttttccgata agctccgaaa cgcccttata gatctttgac attttaatct
      901 acctccgata tgaaaattat tttatgcggt tttctgtacc gcttatgttt gctatggtta
      961 tactatatca ctttattcct actatgtcaa taggattaat atgctttctg tgttttatac
     1021 aacaaagtca gcttattgca agcttcgatt tgtgcattat gacctataat catactcagt
     1081 aatgcaatat ggcttattat tttcatcgca aagctccaca aattttctgc cctcatcggt
     1141 ataaacaaga gcctttatgg ctttaccacg cttcaaggga tttttatatt ccacacggat
     1201 acgcttcaca gcagactcgt tttccacaaa ctcgtctgtt atgtcaagat atctcgcatt
     1261 attgacgtgt tcgttcatgt cgatatagca tttcagcacc gggatatcct catacacctg
     1321 tggctcagta tcaggtaggg ctatctttct tggaagatac tccatatcaa gctttggata
     1381 gagcttcacc ctatcaacaa gctcctgtgg agcttttata ggccgctgag aggtcttgtc
     1441 catgaaagcg ccagaagcta tagacttcac gcagatatca ccgttttcgt catatatcac
     1501 ggtgtttctg tagccataca ttcttttaag ctcataggtc catgttttga cggtgagctt
     1561 ttcgttttgc tcaggtctgc ggacgatatc aagctgtctt gacacaagaa acatcacgca
     1621 gttttctttc tcaaaaaaag gcgacataac agggtgagtg tcaaggtgga taacagagca
     1681 atcctgcaaa aggtcaacaa ggcaactatt tcttattatg ccatgcgaac ctatctgact
     1741 gcaatttaca gtgcgtttca tttcatacat attgctttcc tccattctgc tatatgataa
     1801 ttatacaact tattgtcgct tttttcaaca gcacataggt acaaatgtta acagaggtaa
     1861 agaaatgtta aaaaaaagct tgttgaaatt agtccgcttg gggacttata atgatattac
     1921 agcaattcta caggaggcaa aatatgtttt tatggttaat gatagctatt cttgtggcgg
     1981 tctgctctgt ggctggcttt atttatctca caagaagaat atcagctttt ggctttataa
     2041 aaaagctttc aaaggataaa aaggtactta aatacgccat aagtgcaggt cttatagtgc
     2101 tcacaggcgg cataatatgg cttatatggg actggataaa tgcgataatc tgtttgttgc
     2161 atcttgttat attctggctt ttgtgcgact tgtttgtgtt cattggaaag aagatatcaa
     2221 agaaacaggc aagcaaatgc cttgcaggct ttatcgccgt gccgctgacg gtggtttatc
     2281 ttgcggtggg ctggtatctt tgcagcaatg tatgggaaac tgactacaca ctgaaaactg
     2341 ataagaagtc aggtgacata aggatagttc aatttgcaga ctctcacgtt ggcacaacgt
     2401 tccatgggga cggcttcgca aagtacatgg aagagataca gaagcttgac cccgatgtgg
     2461 tgcttataac aggcgacttt gttgatgatg acacatcaaa agaggacatg atacagnnnn
     2521 nnnnnngctc ttggcaagct gaaaacaaaa tacggcgttt atttctcatt tggcaaccat
     2581 gacaagggat actatgaccc ttcgtatcgt ggctacagcg gtgacgatct tattgcggag
     2641 cttgaaaaga acaatgtcaa cgtattacag gacgacaccg tgcttatcga cgatcgtttc
     2701 tacataatag gcagacagga caggtctgag gaaatggaaa agggcggtca cagagcatct
     2761 atggaagagc tcacaaagga tcttgacagc tcgaagttta cagttgtaat ggatcaccag
     2821 ccttgtgatt atgacgctca ggcggcgtca aaggtagacc ttgttttgtc aggtcacaca
     2881 cacggcggtc agctcatacc aataaacttc cttggcactc tcatcggcgg caatgaccgc
     2941 acttacggct atgaaaagcg tgaggacacc aactttatcg tgacatcagg catttcagac
     3001 tgggcgataa aattcaaaac aggctgcaag tctgaataca ttgttattga tgtttcggag
     3061 aattaaacag caggacaaag caaaagggtc agcgaaaatg ctgacccttt gttttatttg
     3121 aacacaagct gtaccaaaag catatagcac actgttccgc ttgcaataga caaaagcatt
     3181 tgtcttttcc acaaatgcac tgtcactgtc acgcctatgg cgataagttc aggcaagccg
     3241 taactgtaat gcagaggtgt tacgtttcta aggcaatata ccacgagcat actgaatatt
     3301 gcgcttggaa gcaccttgcc caaatactgc acatagcgtg gtgttgggcg tttgccgttg
     3361 aaaataagaa acgggagtgc tcttgtgaga acagtgccta aaacgcacat tcctatagtg
     3421 atgacctgct gagttatcgt cactttgcgt cacctgcttt ctcagggttt tctataggct
     3481 tgcgaaaagc cgtgagcaga acgagaatac atatcattgt gggtatcatg aaatcgtctg
     3541 taccaaatag tataaggcag acaacgcttg atatcaagcc tatccatgca ctcatatggt
     3601 gtttttcttt gagccactgg tcaagaaata tcacaacgaa caaagccgtc attgcgaagt
     3661 caagtccttt ggtatcaaag cttataagat cacccataag accgccgata gttgcaccgc
     3721 tcacccaata tatctgattg agtagcgtaa caaaaaagta aaaccaacct ctgtcaacgt
     3781 ccttggggat ctctgctgag tagtttatgc tgaaagtttc gtcgcacatt ccatagataa
     3841 gataatattt tttccagcca agccctttga acttatccag cattgaaatg ccatagaaca
     3901 agtgcctagc ttgtatcatt atcgccatta tcaaggtctg cacaggcgca aagggtgaaa
     3961 gaagcatact tactgtgata aattcaaggc ttccgccata tatcgtcatg ctcattatga
     4021 gggggtagac aaagctgaat cctgatgcgt tagcataaat gccataggca atgcctaaaa
     4081 accaaaaacc tgcgaatatc ggtattgtat aaggaaaagc ccatttcagg gctttgaatg
     4141 cttttctata gtgatttttt tcaaaatgtt catctgcctt tcgggcgttc gctgtgtcga
     4201 aaaacgctgt gtataaaagt ttagcacaat gtttacctgt agtcaagcac cgaactattg
     4261 acaaatcgca cttgtggata tataatttac caagaaacta cactttgaaa aggaggtgtg
     4321 gcagtgacaa aggtcatata tcctgttatc ggcaggcaga cttccctgcc cttttatctc
     4381 accggaatag gcatttccga ccctgaatat cacgtcacaa gggacaaagg gcttgtttca
     4441 catcagcttc tttttacctc agggggcgag ggcagactaa ttgtcggagg agaagaattt
     4501 gtgcagacta aaggctcggc tttttatctg ccccctagcg ttccgcatga atattatccc
     4561 tcaaatggaa attggataac aaactggata gttttccgtg gtgaatttgc ggggcaaatg
     4621 cttgcaaacc ttggctttga cagctttaga ttctcccctg agataaacac ccaaaaacct
     4681 gctcagcttt ttgagcgtat ttttgttgcc gctgccgacc ctgtgaattg tggcgaaaaa
     4741 acttccgcac ttgtgtatga atatatcctc gccatgagga ctgcaatgct tttccctgac
     4801 agccgaaaca acgtgggaag cattacaagg caggcgcttt tgtatattga cagccgatac
     4861 atggaggata tcaccgcaga aacacttgca ggactttcgg gagtgtcagt acaacatttc
     4921 tgccgtgttt ttaaagcaga aacttcaatg cgtccacttg aatatattgc aaaacgacgt
     4981 atctctcagg caaaatctct gcttcttaac accgactctg acataggcga cataggcaag
     5041 caggtgggct atgatgacag aaactatttc agcatcgtgt ttaaaaagct ggagggcgtt
     5101 tctccaagag aataccgtcg ttcaagagga acgggtttgt aataatattt taagctttat
     5161 tagaattttc ttagcatatt tcaaagcaaa atgttcgtat cttaataata tgcaaaatat
     5221 ttcagattga ccatacactc ctttaagctt ataattgtca taagaaagca agaagccatg
     5281 ctttatgcaa ggaggataac ttatgacgat actaaaaagg agcgcgtcaa tggtgtcagc
     5341 attggtcatg atgatatcta gcgcaggcgc ttacaattgc agtgcggagg gaacaacggt
     5401 gaacgatagc aatacaaata atacaagaga gatagttatt gacggaacta cggctcaact
     5461 caaagaaaat atgcgctaca gaggggcagg catggtgagc gcaaacaact catcaaggct
     5521 tctgctggat tacaaggctg aacaccctga tgtatatgag cagataatgg agtatatgtt
     5581 tggcaaagag ggtatcggca ttactcacct taagttggaa atgggtgcgg atataaactc
     5641 atcatcaggc acagaaccgt ctgtaatgag aactgaggac gaaaaggctg acgtaacaag
     5701 aggtgcggca taccagcttg ccgctgacgc aaagaagata aatcctgacc tcaccttgga
     5761 tatgctttgg tggagtgaac caaagtgggt atctgacgct tccgatgttt atgctgcaag
     5821 atacaaatgg tataaaaaaa cccttgactc cgcttatgaa acatatggac ttgaatttga
     5881 ctatgtaagt gctgtgcaga atgagcgtgg agttgaccct gaatgggtga aatatctttc
     5941 ctcacatctc aaaagtgaaa aagacacccc ttatgactac tcaaagataa agatagtcgg
     6001 tggagaagag gtctgcacat ggggctttgc tgacagaatg tatgaggaca gcgaacttat
     6061 gaacgttgtt gacgtggtgg gaagccatta cacaagcgag tccaccgaga acgcacagaa
     6121 gcttgcatat gaagaaaaca aagagctttg gttcagcgag gcaagctcac caatggcata
     6181 tgcgcagggt acatacagat atgacggctc aggtctggca ggcataaacg gcacactgga
     6241 tattgcaaac agaataatcg gaatgtatcc aaacggcaaa atgacacttt atgagtatca
     6301 gcctgttgta tcggcatact atgacggcgt ttgctactgt cagaaacagc ttatctcagc
     6361 ctgcgaccct tggagcggtt attatatgct tgacagcgga ttttatatgt ctctgcattt
     6421 ttcgcagttc attgaaaaag gctgggcgtt cgttgacagc ggatgctaca gcgacggaaa
     6481 aaaaggcggc gacggccacg ctatcgtcga cgcagtttac agctatatga ccgccacaga
     6541 cacagaaaca ggcgattaca gcactgttat cacaaacact acaagcgagc ctatacagta
     6601 tgacctcaag gtatcaggac ttgacaaggc gtcaagcaac gtgagcgtat gggaaacaag
     6661 aggtcctgac agcattgacg gcagctatga cgagaattat ttcaagaaaa ctgaggatat
     6721 aacccctaca gagaacggcg gagcttatac atactctgtg acagtgaagc caaattctat
     6781 tgtgacaatt tctactgtta cacctgagcg cacagaatac aaaaacgctg acgagagcga
     6841 acgcacagtt ttgaaacttc catattcgga cgattttgag tatgcaggct atcctgagga
     6901 ttatctttca tcaaggggca atgcgccaag atacacaaca gatcagggcg gtgctttcga
     6961 ggtggaaaag accggcaagg gcaatatgct agttcagcag ataacggctg acatgaaagc
     7021 caacgagtgg ggctacaccc cagaacctgt gactacattc ggtgacgaca ggtggtataa
     7081 ctactctgtg aacgttgacg ctgcttttgc gacttcgcag gacgcaggat caaactatgt
     7141 gggcgttggt ctgagataca cgcttggctg caattcatac agtggctatt ggataaagct
     7201 ttatgagaac ggaagctggg agcttaaagc aaatgacagc actcttagca gtggaagcat
     7261 agacggcttt gacggcacag cttctcacaa gcttaaagtc gaggctgtaa acaacgttat
     7321 ccgtggatat attgacggaa acatggtggc tgaatacact gtcaacgaag gcgaatctct
     7381 tatcggcgca ggcagagcgt cacttttcag ctcatacaac aaaaacagct ttgacaactt
     7441 ctctgctgaa cctgtggagg gcagtgacac atatgtaact cgctttgacg aaacagactc
     7501 ttgcttcact tttgagggca actgggaaca caaccttatg agcagcttca aaaactacaa
     7561 acgaaccata tcagatggca cagagggcga ctctttcaca gtaagctttg agggaacagg
     7621 ctttgcgctt tcaggtgaaa caagagaaaa cgctatactc ggggtatcaa tagacggagg
     7681 tgatgaacag ctcgtgacag tttcaaggac aggtcagcgt gagatatcat gccattttga
     7741 aggcttgtct gagggcgctc acaccgcaaa ggtaaccata aaatcaggca aatatacaat
     7801 tgacgctatg caggtaacag gcggaaagat accatttgag aagaaaaagc ctgcgaagtc
     7861 tgaaagcact aagtcaggca aaagcaagct tcctatagcc gtgggcgcag gcgtttgcgc
     7921 tgctgcggca gttggtgcag tgatatacgg catagcgaga aagaagcgta aggaaaataa
     7981 tgactaaaat aagcagaaca acagacatga cacaaggcag tgaaatgcgg cacataataa
     8041 tgttttcact gccattgctt gcaggaaatc ttttgcagca gacatacaat atagtggaca
     8101 ctatggtggt tgggcgttat ctgggcgatg acgcccttgc cgctgttggt gctacaggct
     8161 ctatcactta tcttttttac accctttgca tagggctgtc ggtgggtgca ggaataattg
     8221 tcgcacagta tttcggtgca ggcaagaaaa aagccctgcg gtcggcaata ttcaactcag
     8281 cgcttgttac ggctatattc ggcattgtaa taagcctttt atccgtgcct gccgcaaaac
     8341 ctatactaga acttttaaac gtccccgaca agcttatcgg agattctgcg gtttatatgc
     8401 agatagcctg cggcggaacg atagcggtgg cagcatacaa ctggataaac gccataatga
     8461 gggctttggg cgactcaaaa acaccgctga tattcttagg tgcagcaagc ttgctaaacg
     8521 ccctccttga cctgcttttt gtggtggtat tcaaattcgg catagcaggt gcggcatggg
     8581 cgacagtgct tacacaggca ctttccgcca tttcttgtat tgtgtactgc tttgcagtga
     8641 gcggagagat aaagctcacc gccgacgata tgcacgccga caaggacatg atgtgccgct
     8701 gtgtgaaaac aggcgtgcct atcgccatac aaaacgggct tatctcggta tcaatggtgg
     8761 ctctccagcg tgtgacaaac ggctttggcg aaatggtcat ggcggcatac acagtttcaa
     8821 tgcgtatcga gcagttcgtt caacagccgt tttcttcact taatgcggcg gtttcaacat
     8881 tcacaggaca gaatatcggt gcaggaaaag aaaaacgtgc gcagaaaggt cttgcttgtg
     8941 caataaagat atcggtgata ttctcggcag tggtgcttgt gctgttcaca ctgttcggtg
     9001 gagcgataac atcttggttt gtcaagggca agactgttat aagcatatcg gcaaaagcgc
     9061 ttatcataac ctcacttttc tactggtcgc tgggtattat ccacaccaca aggggctttt
     9121 tgaacggcgc aggcgacaca aactatgccc ttgtaaacgg catggcagag gtaatatgca
     9181 ggataggcct ttcgcttatc cttacaagga tagctttcat aggctattgg ggcatatggt
     9241 acaccaccgc tgcaacgtgg tttgtgaccg ctatggtgag ccttataaga tacaagggtg
     9301 gaaaatggag gcttcattca attgtaaaat agattttcat aactgctgta ccgattgggt
     9361 gcggcagttt ttttagttga aacagtttca cattatatat gaaatattca tatgaaacca
     9421 tattgaaaat tcatagtgca gtggtacaat ataataaaag gagtgacgag caatgaacat
     9481 taccgaattt gcaaaagccg caggagtatc aaaatctgct gtgagcagat atttcaatga
     9541 cggctatgtg agcgaagaaa agcgcaggct tattgaagcc gccatagaag ccacaggcta
     9601 tgccccaagc gttcaggcgc agaacgtgag gacaagggtg acaaagcttg tgggagttat
     9661 tataccgaag ctttcaagcg agagctgtgc aagagaggtg gagggaataa gtcaggtact
     9721 cagtgagcag ggctatcagc ttttgcttgt gaactctgcc aacgacccaa agaaagaagt
     9781 ggaatatctg gagcttttcc gaagcaatcg tgttgacgga gttatctttt tggcttctgt
     9841 tttcactccg ctccatgacg ctgtgctgaa aaaaatgcgt gtgccggtgg taattgtggg
     9901 acaggaatac aagggtgcaa actgcgtttg tcatgacgat tacaaagccg cctataccgc
     9961 agagaagctt atgcttgaca aaggctgcaa aaagcccgct tttatcggtg ttaccgagca
    10021 ggacaaggcg gcaggtcagg caagaaaaga cgggtttctc cacgcacttt ctgataacgg
    10081 catcaacatt gaaaagtctg atatgcagac ggctgaattt aaaatggact caggctacaa
    10141 aagggctttg acattactga gaagcggcag aaggtacgac cacattttct gtgccaccga
    10201 caatcttgcc gcaggcgcac tccttgcctg ccgtgagcag ggattgaaag taccagagga
    10261 tataatgata gcaggtattg gagactctca gctttgtaat gtcacgtcca ctcccctgac
    10321 ctctgtgcat tttcattatc gcaccgcagg catagaagcg gcacaaatgc tgctcaattc
    10381 acttaagcga gaaagctcta taccaaggac actgacgctc gattttgagc tggaagagcg
    10441 tgagtctaca gagaaaaaat gtgaaacctt gtaatacctc cttgtaagtt tatattattt
    10501 gtcaatagac aaatgcactg tcatgggtta taatatagtt aatgaaatcg gtatcacata
    10561 aatgatgaaa ctgataaaat aacatagttc ggacaaaata ataaaaaagc aagcaaagga
    10621 cgggatatta tggatcacag aagttcagct cgtgagatac tttctgctat aggcggaaag
    10681 gaaaatctgg tcagtgccgc acactgtgcc acaagattaa ggcttataat cgcagacaac
    10741 tcaaagatac aaaaggatac cctggagaac attgatgttg taaagggcgt ttttgaagct
    10801 tcaggacagc ttcagatcat cataggcacc ggaactgtaa acaaggtcta tgacgaattt
    10861 atcgcagagg caggcgtgga agcctcttca aaggacgaag tcaaggctca ggtggctcag
    10921 aagcagaact ggttcaaaag acttatcaaa actctcggcg atgtattcgt acctataatc
    10981 cctgctatcg ttgcaggcgg tcttatcatg ggaataatgg aagccttgaa tttcatggct
    11041 tcaaacggct ttatctccct tgacacaaat ggatatgttt tcacaatatt caaactgctt
    11101 tcaaatgcag cgttcacatt cctgccgata cttattggct attctgcaac taaggtgttc
    11161 ggtggcaacc cttatcttgg tgcggtaata ggaatgttcc ttatccaccc tgacctgcaa
    11221 aacgcttgga cagtttccac aaacggatat gagcagactt tctccatatt tggtctgttc
    11281 aacgttcaca tggtaggtta tcagggtcat gtaataccta tcataatcgc ttcgttcatt
    11341 cttgcaacta tggagaaaaa gcttcacaag aaagtgcctg acattgtcga ccttttcgtt
    11401 acacctcttg tgagccttac agtaacagct tttctcgttt acacctttat cggtcctgta
    11461 ttcgttgccc ttgaaaatgg cattatcaca ggcgttcaga agcttatcac tatcccattc
    11521 ggcattggtt cgttcataat gggcggtttc tattcaacaa cagttgttgg cggtatacac
    11581 catatgtaca ctgttatcga cattggtcag cttgctaaat atggtgcgac atattggctg
    11641 cctctggcat ctgccgcaaa catggctcag ggagccgcct gccttgcagt ggcagtaaga
    11701 agcaagaaca ataagataag atcaatggct tatccttctg ctctgtcttg tatgcttnnn
    11761 nnnnnnngta tgcttggcat cacagagcct gcaatattcg gtgtaaacct tagatttgta
    11821 aagcctttca tattcggttc tatcggcggc gcttgcggag caatggtcgc ttctatcctc
    11881 caccttggcg caactgcaac aggtgtaaca ggaatatttg caatacttct tacactcaac
    11941 cacccagtga agtatataat atcaatggct gtagcaatgg gcgttgcatt cactcttact
    12001 tggcttttcg gcacgaacaa cgagcagata aaaaacgaga acaagccttc cgaaaacaag
    12061 acagaaaagg ctgaacagtc tgcacaggct atggaaacaa aggctgaaaa tgcagacgta
    12121 gcagatgacg gcattatcac agctccgctt agcggcaagg ctgtaaagct tgaaaacgtt
    12181 cctgaccaga cctttgcgca ggggatactt ggtcttggtg cggcaataga acctactgag
    12241 ggcaaggtcg ttgcacctgg cgacggcaag gtcgaaacgc tttttgacac tttccatgca
    12301 gttggtttga cacttgacaa cggcaaggag cttcttatcc acgttggaat aaacactgtt
    12361 gagcttaaag gcgagggctt caaggcttac gtttctgagg gcgacagagt aacaaaggga
    12421 cagacgcttc tcactttcga tatggacttt ataaagagca agggctatca gactatcaca
    12481 cctgttcttg tgggcaattc agacgaattt gcctccgttg aggagcttgc agaagaaggc
    12541 acagaagtga ctataggaga taagctaata aaggtaaacg cataaagtac agacagaccg
    12601 tcaataatta tagtcaacgc atcaaaagga gaggatataa atggataaca aagaaaaact
    12661 ggaaaacaga gaaagaataa agaacgcaaa atggcgtcag agatttcata tcgaaccgcc
    12721 tgacggttgg ctcaatgacc cgaacggtct gagcttttac aagggcgaat atcatgttta
    12781 ttttcagtac agcccaattg ccgctgacgg tcacacgcca agaggctggg gacattatca
    12841 cggcagcgac cttatgcaca tgacttatga cagagcagtt atgatgcctg acattcctga
    12901 ggacagccat ggtgtttact caggaagtgc cattgaaaat gacggtgttc tgcatatttt
    12961 ctacacaggt aatgtgaaga tgataggcga ttatgattat gtcaaagccg gcagaggtgc
    13021 aaacgttatc cacgtcacca ccacagacgg aagcaaaatg agtgaaaagc aggtacttct
    13081 cagaaacagc gactaccctg atttctgctc atgccatgtg agagatccaa aggtgtggaa
    13141 agaaggcgac atatggaaaa tggtgctggg tgcaagaact cttgacgacg agggatgtgt
    13201 gcttgtatac caatctgacg atcttataaa ctggaaatac accggcaagg tatacaaaga
    13261 gggctacggc tatatgtggg aatgccctga ctatttcgag atagacggca agggcttttt
    13321 aagcacctgc ccgcagggta tgcctcacta tgaaacaaag tggcagaacc ttaacgaatc
    13381 aggctacttc cctgttcagg gcaagcttga ggacagcgtt ctcggtgact tcaccgagtg
    13441 ggacatgggc tttgacttct atgcaccgca gacgttcctt gatccacagg gcaggaggat
    13501 actcataggc tggcttggca tggataacaa ggtctacggc aatgccacaa ctgagcttgg
    13561 ctggcagcac tgccttacca taccgagagt tgtaactatt gcacccaacg gcaagctcag
    13621 acagcagcct atagctgaat ttgacgagct taaaagcaac gcacgccgtc agagcagcgg
    13681 tcaaactgca gaatatcctc tgccatttga acttgacggt gagcctgcgg atagcttcag
    13741 catatccctt gacggcaagc tggagcttgg ctttgataaa gagaaaaagc tctttacaat
    13801 gagatttacc gatgaaaaat acggctgtgg cagaacggta agaaatgcag agatagacag
    13861 cgtgagaaac ataagagtta tcgcagatat gtcaagcatt gaggtatata tcaatgatgg
    13921 tgaaactgtg atgtcaacaa gaatatatcc cgataatgac agagtaaagc ttaaagtaaa
    13981 cggctttgaa gcacaggttt gggatatctg agaggaggaa actgcaatgg gaaacacact
    14041 ttttgccata ggcgaagcac tgatagattt tattcctgca cagacaggct gtgcctttga
    14101 agaagtaacg gctttcagcc ctaaaagcgg cggagcgcct gcaaacgtct gcggagcgtt
    14161 cactatcctt ggtggaagct cgaaatttat cactcagctt ggtgatgacc cattcggaag
    14221 aaagatagca ggcgaccttg caagggctgg aatcgacact tcccttgtga gcttcaccga
    14281 caaggctaac acagcattgg cttttgtgag ccttgaaaat gacggcaaca gaacgttctc
    14341 attctacaga aagccctccg cagatatgct tttttcagcg gagcaggtaa aagaagaatg
    14401 gtttgaggac gcatacgcac tgcatttttg cagtgtttct cttggagatt ttccgatgaa
    14461 agacgcacac aaaaaggcaa tcgcctatgc acgtcaaaaa ggcgcaatga taagctttga
    14521 ccctaatctg cgctttcctc tttggaacga caagaaagct ctgcatgata ccgtgaatga
    14581 gtttatacca atggctgaca ttgtgaagat atcagacgaa gagcttggat ttatcacagg
    14641 tgaaaacgac atcaaagccg ctctgccaaa gcttttcaca ggcaatgtga aacttgtgat
    14701 atacacctgc ggctcagagg gagcatatgc tttcacaaag aacgcacagg catttgcaga
    14761 gagcgaaaag gtgaaagccg ttgacacaac aggtgcaggg gacggattta taggctcgtt
    14821 cctatatgca ctaaaaactc tcaatgtgac cgctgaaagc ctttgtgacc ttgacaatgc
    14881 acagctagaa cgctgtatag cgttttcaaa taaattctgc ggtgaaagcg ttaaacatca
    14941 gggcgctatc gactcctaca gaaaaataga atttgtataa atttgcataa taattatcct
    15001 ttaataatac atttccttaa aacaaagcct tccccttttc gggagggttt ttgttttcgg
    15061 caaaatttca caaaacattt tgcagtatat gtaggggacg gcgccctcga cgtcccgctt
    15121 ttgcgttttt gaaaggcttg ttttttaaca cgttttagca tagtaatatg atactttgtg
    15181 aaaatttgac aaaatccacg atatactata taatagagat tgtatgacta ttttacagga
    15241 gaattattat gaacgaaaat aaaaacagag caacctttca atcacgattg ggatttatac
    15301 ttgtgtccgc agggtgtgcc attggtcttg gcaacgtgtg gaaatttcca tacatttgcg
    15361 gacaaaacgg cggcggagct tttctgttcc tataccttat ctgccttgtg ctgttgggtc
    15421 ttccaatact catatgcgaa tttgccatag gcagaggaag caaccgaagt ctttctcagg
    15481 ctttcaaccg acttgaaaag ccaggtagcc gatatcactg cacaaaatac acgagcattg
    15541 caggaaacta tctgctcatg atgttttaca ccatggtcac aggctggatg ttatactacg
    15601 ccttcaaata cgccactggc tcgttggacg cttcaagcac agcggcaaca gctgaggctt
    15661 ttaaggatat gcaggcttcc ccgtggctca tgctcatatt ctcggctatc gttatcatac
    15721 tttgtatcgg tgtttgcgca ctgggattgc aaaacggcat tgaaaaggta acgaaattta
    15781 tgatgatact gcttatggcg ctcatggtgg tgctggcggt gaactctatc aggctgaaag
    15841 gcgcttctga ggggcttaaa ttctttcttg tgccggactt tgacgtcttt aaggaaaagg
    15901 gcttcacatc agtgcttttc gccgcaatg
//
LOCUS       DAWEMR010000045        15552 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1583_length_15552_cov_11.449506,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000045 DAWEMR010000000
VERSION     DAWEMR010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15552)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15552)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15552
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1583_length_15552_cov_11.449506"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3986 a         3498 c         3434 g         4634 t
ORIGIN      
        1 tgcatgaact tatcgagaag atagttgttt acgctcctga aaagagcagt gggcaccgca
       61 cacagcagat tgacatttat tatcgcttcg atgttgcagt atcgactgcg gttgctgaca
      121 gtatgaagta cgacaaaaag agaaaggctg cgtaaccaag gcggttacgc aaccttatct
      181 tcaaatcata aaaacttctt tacgagtacc cgtcttatcg gagagctttt ggttatttta
      241 cccttatgat aggctgaagc tgtatcccat caagagccga ctgaatagtt tcgggtatga
      301 gttcaaattc agccactatg gaatttggct tcttttcgca gttgtcctgc gcatatggta
      361 cgaaatagta gtttctcaga ttttgcagat aacctatgtt tttagcacac cctgccagtg
      421 cgtcatttgt cgatatggcg atgaccacgg gacgagcatt tctcaggtgg ctttttaccg
      481 ccatggtggc tggagtgtcc gtcaagccaa gagcaagctt tgctgccgtg tttgaggtgc
      541 agggtgctgc taccatgata tcacacatct ttttcggtcc gatcggctcg gcttcttcta
      601 tggtgcgtat gaccttgcgg cctgctatgg tttcaaggcg tttgatgttt tcttccgctg
      661 tgccaaaacg tgtagatata gatgcggcgt tgaaagacat tattggcaca agctttgcgc
      721 cctgctgcac aagccgttct gcggcggcaa atgccttttc aaaggtgcag aaggagccgg
      781 tcatgcaaaa gcctattcgt atgccgtcaa gtgacattct ttgttcccct ttcattcaaa
      841 atgtccgtga ctgttcgggc gattatctct cctgcagtca taggtgcggc tttggctgga
      901 agtcccaatg cccataccac acgcctgttc agctttgcgg cagtttcaaa atctactccg
      961 ccaggtcggg aggcaaggtc tatgataaga gagtttttat ccattacgcc caaaagcttt
     1021 tcatcaagta tcatggcagg gacggtgttt attattatgg tataccccga aatattgtcc
     1081 gcaagagctg tgataagttc tgccttatag cctgcgccct cggcttctgc aagcgcagaa
     1141 agctttcttg cggatatagt cacatctgcg ccgacagccc gaaaaagctt agcgcaggct
     1201 ttcgctactc tgccatagcc aataaccagg acggtggaac cgttcacggt aacagcaagc
     1261 tcctgcatgg ctatttgcaa agcaccctcc gccgtgggga tacaatttct tataacaagc
     1321 tcctctcgct tatagtaatc cttaacatcg aagccgagag aggagaaata ttctatttga
     1381 tcacggcgaa gcagaccgcc tgccacgagt gcatttttct ttaaatgcgt tgaaacgtca
     1441 ctgcatgata cctttacatt acggctagaa gagatatcca gcccgtcagc tttcataaga
     1501 ggaagtacga gaacgtccgc cttttcggtg agctgatcga gatgagcgag ggggatagtg
     1561 tcctcgcctt tatcgtccat gccgtaagta tagactctgc cgattttagc gagagtctga
     1621 gctgtatatg tttgtcttgc gtcaccgccg acgcagagga aaactgtttt tttcatatgg
     1681 atacctcctg tgggactatg cctgatagag ttatatataa ttatatgtat cggtgggcta
     1741 aatgttaccg gcaaaacaaa acgccctatc cctgcaaagc aagaattgcg gcgctttgat
     1801 atgatatcat atttctatct tgataaactt cggctcgtgc tttttgaaat aggtcagata
     1861 cttaaagcct gccgcctgcg ccatttctac gcctgcggaa atatctctgc cgatatctgc
     1921 ggtgcagtgg gcgtctgagc ctatggtcaa tatctcgccg ccgagggctt tgtagagctt
     1981 cacatagcca aggtccggga gtggcttgcc gtacttctgc cttaatcccg acacgtttat
     2041 ctctatgccc ttgccttttt ggataagtgt acaaaaaatt tctctgataa tatcttcata
     2101 tgtagaaaga tctatttgta taccgcaatc tccttggata tatcgcagag gataggttag
     2161 gtgtcctaaa acgtctaact tgccccattt gcacatttca agcatttctt tgaaatagtc
     2221 gtcaagaaga gcataggttt gcgacaagtc catatttttg tactctatcc aatagaaatc
     2281 gtccttgcat ttattctgat gatgtgatcc gatgataaaa tcaaggcgtt tgtcggaggc
     2341 tatctcttcg gcaaattcta tatcctgcaa aggctgacca agctctattc cgcagagaaa
     2401 atttaaaccc tccagatgct gggaagcttc atactgggcg actatgcttg cgggggcata
     2461 ctcccccgag ccgtacatca tgccgtcttt cagggcttca gggtcaccgt aatggtcggc
     2521 tttgtagcga aaattgcaat cgcaatggtc agttatggca aatgccgcca agccaagctc
     2581 tttggctttt gctaaagact gttctacagt tccctcacca tctggggaaa agcctgtgtg
     2641 ggtgtgcata tctataagag ttttcatagg ttctcctatc tgtacagaaa tttgtactta
     2701 tatagaattg taaagctttt caagctgttt tgccgcttct gccgcacgtc ttacaaagct
     2761 gtcaggtgca gcatttcggt acatggtgga ctttgacata taaagctcta atgacaatgg
     2821 tagctggtgg tcgaggtttt tgagctttct gagaaaattc tcaaagttca ggttgccgtc
     2881 aaacggtatc atgtgtccgt cggtgttgcc gtcgttatcg tggatatgca ggcaggcaag
     2941 cttgcttcca aacttcgtca ggcaatcggt attctttgag aaacaattct catgacctgt
     3001 gtcatagcaa aatttcacgt tatcatggtt tccaaaccgg tcaaaaagca cgtcaagaaa
     3061 ctgcgggagc tggatattct caaaggcgat atcgacgccg ttttcttctc cgcactgtat
     3121 tatcttggcg aaattctcta tgccaaaacg agatacctcg ggcggaacaa gcccgtcagt
     3181 ggggtggcat atcatggttt ttatgccgtt ttcactgcac actcttacac attcaagtgt
     3241 tcgttggagg atatattcgc tctcttggca tattgtccaa agggcgttca tgcggtcaaa
     3301 ctgtgaatgg acgttttcta tttccagtcc aaaatttcgt acaatatcat actgtgtgta
     3361 ttttagtccg tcagaaaagc caagcattac accgtcgaag ccgttctctt ttataagtct
     3421 tatacgctcc tcaaagggta ttttaaaggt gaagtctgca aacatacagc gtttcaatct
     3481 gttttcctcc ttaccaaaaa cagcggcaga attgctctgc cgccgtgaaa atttagtttt
     3541 tgagagaatg gagtggtgca gggatctgtc cgccacgatt gatgaacttg gaaggtgact
     3601 cttttttgag cggcataaca ggtccgcagc caaaaagtcc gccccaatca agttcttcgc
     3661 caacaccctt gccaatagca gggataactc ttacggcggt ggtcttgttg tttaccatac
     3721 cgatagcagc ttcgtctgcg atgatagctg aaatagtatc agctggggta tcgcccggaa
     3781 tacatatcat atcaagacca actgagcata cagcagtcat agcttcaagt ttttcaatgc
     3841 agagcgcacc gctctttgcg gcatcaatca tacctgcgtc ctcagaaaca gggataaatg
     3901 cacctgaaag tccgcctatt cttgagcaag ccattacgcc gcccttttta actgcgtcat
     3961 tgagcagggc gagggctgct gttgtgccat gtgtaccgca cttttcaagt cctatttcct
     4021 cgaggatatg ggcaacagag tcgcctacgg ccggagttgg tgcgagtgaa aggtcaacga
     4081 taccgaatgg aacgccaaga cgcttggaag cgatagttcc aacgagctga cctactcttg
     4141 ttatcttata tgaaatctcc ttgataacgt cggatatctt tgtgatatca gcgtcaggat
     4201 attttctcaa agcggctctt acaacgccag gacctgatac gcccacgttt atcatgcaat
     4261 caggctcgcc aacgccgtgg aaagcacctg ccatgaatgg gttatcctca actgcgttac
     4321 agaaaacaac gagctttgct gcgccaaagc aggcagagtc aactgttctt tctgcacact
     4381 ctcttacaac gccacccatc attttgcagg cgtccagatt gatacctgtt tttgttgaac
     4441 cgatatttac agaggaacat actctctcag tagtagcgag tgcctctggg atagacttga
     4501 taagctcgat atcgcctgcg gagaagccct ttggaacgag ggctgtatat ccaccgataa
     4561 ggtttacgcc aacgtccttt gcggctctgt ccatagcgtg tgcaaatgga acaggtgaac
     4621 acttgcaggc agcagacacg atagcgatag gggtaacgga aattctctta tttatgatcg
     4681 ggattccaag ctccttttct atctgctcgc ccacctcaac aagccttgcg gcttttgcgg
     4741 ttatcttctt atagacattc tcgcaggcaa cgtttatatc gctgtcgata caatcaagca
     4801 gagaaatgcc cattgtgata gtacgaatat caagacaatc gtcctgtatc attcgtattg
     4861 tttccaacac gtcatatgta ttaatcattt tacagaaatc tcctctttag atatggtgca
     4921 tacagttaaa aatatcctcg tgcattgtat ggatctttac acccattgtc tgaccaagct
     4981 ctgtaagtcc gtctgccatg gtagcaaaat cggagttgag ctttgtgatg tcaacaagca
     5041 ttatcatagc aaagaggtcc tgcaaaactg actgagtgac ctcgataaca ttcgcattat
     5101 attctgcaca ctttgtgctt acctttgcaa ggatacctac agcgtcctta cctataaccg
     5161 taacaacagc tctcattaat aagaccagcc tttccaaatt catttatata ttttctatta
     5221 taccacacag cggagatttt gccaagagtt ttaattataa atattttatt aacgttggaa
     5281 atgcgtttta tggggcgacg gtcaaggcag gtggtttgcg gtgaacgaaa cgggcgaaca
     5341 ttgttcgccc ctacccatat ttttacgaat tacaaaaaaa ttcgtaaaaa aaatcaaaaa
     5401 cgttgaagtg caaaaaaagc ggggtgtcag atttctgcaa aacaaaaaaa agtttgatga
     5461 gcgggacgtc gagggcgccg tcccctacaa aaaacgcccc catttagggg acgttcttgt
     5521 attatttttc tgttctgaat ggtgcaagtg gtatgcctgt ttcgttgaag atagttacct
     5581 cgcaatagtt gaaccagtta tatcttgcat agagaggttt ttcaacggcc tctgacttga
     5641 tgaaaatttt gctgccgttt atggtgatat cggcttttac gaactcttcg tccttgcctg
     5701 ctatctcaaa gccctgtgct tcgcccttta ctacaaagcc gttttcggca tggtcaaagc
     5761 taagctctat accgccgttt ttatactcca gcgacttata gataggtccg aatgctttta
     5821 cgtccatgcc gtagaaaagc ttttctgcct gcaaacaaag tctttcgcca acgggtactt
     5881 tattctttgg gtgaatctcg ttgaactcac cgcaatctga gataacagct attcctgtat
     5941 ttttcactgt tttatatgct ctcatctgtg cttctcttat tttacaccaa tgcttataat
     6001 cagggtcggc ggcatatttg aacattggga gctggacgat gatgaaagga agttcgtcgt
     6061 cgccccactt ctctctccaa agcctgataa gagatgtgag gagggtataa tagctatccg
     6121 gtttatggtc gtctgactcg ccctgataat agaggaagcc ttttattgta taaggacaaa
     6181 ctcttgacac catggtctca aaaagtcctg ttgggcggaa tgggtttacg cagtttattg
     6241 ggcccggcca attcttatcg ccaaggatct cgttggcttt ctcccagctc attgttgggt
     6301 ctttttgcag aagctcgccg tattttttat cccacacacg ctcaaatttc tgataatctc
     6361 tgtattcttt tatctgctcc tcgacagttt tgccctcaat acgtttttca tactcgtcta
     6421 tgtagcttct tatttcagcg ttgttttcaa gggtcggtct gtctacccat gcggaggctg
     6481 atgtgccgcc ccagttacag ccgataagtc ctactgttac gccaaggtct ttggcaagct
     6541 ttcttccaaa gaaaaagccg actgcactcc atgcccttga attatccttt gaagcctgcg
     6601 accatgcggt attgttttcg tcctcataga acatttcgtc aagagtgcca cgcttttgag
     6661 tgtaataaaa tcttacgttg caatcagggg tgaggttttc gaggacttct ttgccgccct
     6721 ttgcgttttg aagttcaagc tccatatttg actggccgcc gcagaaccac acttcgccta
     6781 tcatgatacg gcagaatttt ttctctgtac agccgtctga aactgtcatt tcgtactctc
     6841 cgcctgcact cattggaggg aggacggctg tccacctgcc gtcgcagacc tgcgtctgga
     6901 ctgacacgcc gttaagagta actgtgacag ttctttcctc gtccccaagt ccccagactg
     6961 ttatgttctt gtcacgctgg agcaccatat cgtttgaaaa aatatttgct acctttaaca
     7021 tactttttct cctttcaata tctataaaaa ttcaaaaaaa catcatcgcc gcaggcgata
     7081 ccttaattcc tcactcctaa ctcctcacta atcaaagagt gtggtagaaa agaatgatat
     7141 cctcaaagac gccttcctta ttttcaaagc cctgcggaac tgttccgagg cggacaaagc
     7201 ccaatgcctc gtaaagcttt accgccttga cgtttgatgc aacaacggca ttgaactgga
     7261 gcaccttaaa gcctttctgc tttgctatga aaagacaatg ttcgactatg acctttcctg
     7321 ctccttttcc acggcaggtg ctttttacgg catagcttgc gtttgcgata tgtccgcatc
     7381 tgccaacgtt attagggtgg aggatataca ttcctacagt ttcgccctca tactctgcaa
     7441 caccacagaa tgtttgtgaa gcaaagaatt tttttgcacc ctcatagtcg agggtctctt
     7501 tctgcgggaa ggcattgccc tcttttacaa cttcgttcca tatctctgcc attgcaggaa
     7561 cgtcatcaat attgtagcct cttacggtca cgtttttcat ttttgccatt ttggatactt
     7621 ctccataata aaattactct ataaatgact tcatgccacg ctcacgtcgc caatcaagga
     7681 actcctcata tgttccctgt ctgtcgatac agccgtcggg ggttatctcg attattctgt
     7741 ttgcaactgt ctgaactatt tcgtggtcat gagatgcgaa aatcacaacg cctttgaaat
     7801 ctctaagtcc gttatttacg gctgtgatag actcaagatc gaggtggttg gtaggtctgt
     7861 caagcatgat aacgtttgag ccgaagagca tcatacggct gaacatacat cttacctttt
     7921 caccgcctga gagaactttt acaggcttat atatctcgtc ccctgagaag agcattttgc
     7981 cgaggaatcc tctgaggtag gtgtctgtta cttcggaagt ggaatatttt cttatccagt
     8041 caacgatgct ttcgtcatta tcgttaaagt aggctgagtt gtcgataggg aaatatgaac
     8101 gtgatgtgga aacaccccac ttaatgctgc cctcgtctgg ctcttcttcc tctgcaagta
     8161 tcttgaaaag cattgtgata gcctgctcgg actcgcctat gaaagcgacc ttatcggtac
     8221 ggctgagggt aaaggaaaca ttatttagga gctttacgcc gtcaactgtt ttggatatgc
     8281 cgttgacttt gaggacgtct tttccaagct ctctatccat attaaagcct atgaaaggat
     8341 attttcttga tgaggcaggc atttcctcga ctgtgagctt atctagaagc tttcttcttg
     8401 cggtagcctg cttggacttt gatttgtttg ctgagaagcg gttgatgaac tcctgaagtt
     8461 ccttgatctt ctcctcggct ttcttgttct ggttctttat cattctctgc atgagctgtg
     8521 aggactcata ccagaactcg tagttaccaa catacatttt tatctttgta tagtcgatat
     8581 ccacgatatg ggtacaaaca ttattaagga agtgtctgtc atgagataca acgaggacag
     8641 ttccgaaata gtcggcaagg aagtcctcaa gccagctgat agcgtcgata tcaaggtggt
     8701 ttgtaggctc gtccagcatg atgatatcag ggttgccgaa aagtgcctgt gcaagaagca
     8761 ccttgacttt ctcgttacct gagagggtgg acatctgtga atagaggata tcctcggaaa
     8821 gaccaagacc ctgaatgagc tttgaagcgt cagactcagc ttcccagccg tttaattctg
     8881 cgaactcgct ttcaagctgt cctgctctgt cgccgtcctc ctcggtgaag tcctccttgg
     8941 cgtaaagctc gtccttttct ttcattatct cataaagtcg cttattgccc attattacag
     9001 tttcaagaac tgtatactcg tcaaatgcaa agtgatcctg acgaagcact gaaagtctgt
     9061 cctcttttga gatcactacc tcgcctgtgg taggctctat ctcaccgcag aggattttca
     9121 gaaatgttga ctttcctgca ccgtttgcac cgattatgcc atagcagttg ccgtttgtaa
     9181 atttaaggtc aacgtccttg aaaagaagct gaccaccgaa tgaaaggctt acgtttgata
     9241 ctgttatcat tttaactctc ctatatctga taattatact ttgacacttt attataccac
     9301 acaaagcggc ttatgtctac ttttaattat taatttttca tcactgcgct tttatggtgc
     9361 aaacgaggtt ttgctcttga cgttttcaac aaaaagtgtt ataattgtta tatgcacatt
     9421 ggcaatagcg ataattattg ccctgaaaat tcaagaaatt aagttggaaa ggataacttg
     9481 aatatgaaaa aagttttgtt tgcttcttcg gagtgtgttc cgtttatcaa gacaggcggt
     9541 cttgccgacg ttgttggctc tctgcctaag tgctttgaca agaggtatta tgatgtcaga
     9601 gtaattcttc ctaaatattc ctgcatgaaa cagcagtgga aagacatgat gcagtacaaa
     9661 acgcatttct acatggactt tgcaggtcag agccaatatg tgggcgtact tgaatgcgtt
     9721 tatgatggcg ttacgttcta ttttatcgac aacgaacatt atttcaccgg tgacaagcct
     9781 tacactgagc atcactggga tatagaaaag ttcatgtact ttgacagagc tgtgctttct
     9841 gctctgccgc ttcttgattt ccgtcctgac gttatacact gtcacgactg gcagacgggt
     9901 cttatacctg tatatcttca tgacagcttt gcagggggag atttcttccg cggcataaag
     9961 accgttatga ctatacacaa tctgaaattc cagggaaatg acggagttga gcagttcaag
    10021 aggatatccg gtctgtctga ctactacttc actatcgaca agcttagaac gggcgttgac
    10081 ggaaatatgc tcaagggcgg tcttgtttat gctgacgcta tcacaactgt cagcaacaca
    10141 tacgcagagg agataaagac gcagttctac ggcgagggtc ttgacggact tatgagggca
    10201 agggctaacg atctccgtgg tatcgtaaat ggtatcgact acgttgatta caaccctgac
    10261 acggacaaga acattgttgt aagatacaat tatgacaacg tgcttgagaa gaagatacac
    10321 aacaagagag caattcaggc tgagcttggt cttgcgcagg acgacaatcg ctttatgata
    10381 ggcattgtta gccgtttgac agatcagaag ggctttgacc ttattgcata catgatggaa
    10441 agaatgtgtg aacagggcat acagttcgtt gtgcttggca cgggcgaaca gaaatatgag
    10501 gatatgttcc gctggtttgc aggcaagtat cctgacagag tttccgctaa catttactac
    10561 agtgaggaaa tgagccaccg catttacgca tctgctgacg cattccttat gccgtcactt
    10621 ttcgagccat gcggactttc acagcttatg tcactgcgtt acggcacgct gcctatcgtt
    10681 cgtgaaactg gcggtttgag agatactgtt cagccgttca atcagtttga gaggaccggc
    10741 actggtttca gctttgcaaa ctacaatgca cacgagatgt acaacactgt tatgttcgca
    10801 aagtacatat ttgatgacag acgtgacgaa tggaacggac ttatcaaatc tgcaatggca
    10861 caggatttct catggcaggc atctgctaac aaatatcagg aaatgtatga ctggcttatc
    10921 ggctaagata gatacaagaa aagctccgca ggaaaggcgg agcttttttt tatgttatag
    10981 gttttgtggc aagcatatga tttgtcggag atcaggcgga cgacctcaga tcgccgctac
    11041 agaaacaaac ataaaaagcg gtacaggctg caccgctttt gttttgcatt tatcagtcac
    11101 cgaaagcgac gcccagagcc tttgcggtct tgacgagctg gtggtcggca gggacgaatt
    11161 ttgttttgcc tgcgacttct gaaagtgggt tttctgacac aacttcattt atcattgcca
    11221 ctgcatagcc gtatttatcg gctgcgataa gctctgcggc atggacaccg aactttgtgg
    11281 caagcactct gtcataggct gacggacttc cgcctctgag catatgacca ggaacgcaaa
    11341 ctcttgtttc tctgcctgta agctcttcta tctgctttgc aattctaggg gtagcagttg
    11401 taaggccctg ctctgcacga agtcttgctc tttccttctt tttaagcttt gcctcttctt
    11461 tatcaaaagc accctcagct acggcgagaa tagaaaatcc ctttgattct ctcttttcgc
    11521 aggcagcggc aagcttttcg atatcatatg gtatttcagg aatgactatc atatctgcac
    11581 cgcctgcgat acctgaatag agggtgagcc agccggcttt attgcccatt atttctgcaa
    11641 cgagtattct tccgtgggaa ttggctgtgg aatgaagtct gtcgataacg tctgtggcgg
    11701 tatccactgc ggtatggaag ccgaatgtta cgcttgtgcc ccagatatcg ttgtcgatag
    11761 tttttggcag gccgataacg ttaagtccct cggttgaaag gaggtttgcg gtcttatgag
    11821 tgccgttgcc acccaaggtg agcaggcaat cgagcttctg ctttttatag gtttctttca
    11881 tagcctttac tttatccacg ttatcttctt cgatgacctg cattttcttg aaaggcgttc
    11941 tctttgtgcc gaaaatagtt ccgcctgtgg taaggatacc agaaaagtca gactgtttca
    12001 tttctttgca catattattg ataagtccgt aatagccgtc ctcgatacct actatctgaa
    12061 cgtcctcgcc gaacttttca tagcaagcct ttgcaacgcc tctgatagtt gcgttaagtc
    12121 ccggacaatc tcctccgctt gtgagtatgc ctattctttt cttcatttta ccaagtcctt
    12181 tcaatgaaat tctatcatta tcaacgatat caatacttat cgcttgtgct gaaatcttct
    12241 atatcggctt cgtcggaagg agtgatatcc tgctgagttt cctcaggggt ggcctcgctt
    12301 tccggctcat cgtcaatata tgtgaacctt gaagtctgat gctctttctt atccttttca
    12361 ctgttctcgc taagtttgaa acgtgctatc atattcttta gtataagcga ctggcttgaa
    12421 agctcttcac ttgcggaagc tgaaccgact gcagttgagg tattggctga tacaacggct
    12481 gatatctggt ctactcccgt atttacctga acgatagctt ctgcctgcgc ctcagaagcg
    12541 tctgagatat tctttacgag atcttttata gtatttgaat tgcttacgat attgttcagc
    12601 gaatcagcag tttcttcggc tatcttagag ccctttgaaa cagcggcaga ctggttttcg
    12661 ataagtgaag cggtctgctt tgcggcttcg gctgatcttg atgccagacg tcttacctcg
    12721 tctgctacca cgccaaagcc ctttgaaccc tctcttgcgg cctctacggc ggcgttaagg
    12781 gcaaggatat ttgtttggaa tgcgatctcg tcaatgacct tgattatctt tgagatctca
    12841 tcggaagctg tatggatagc attcatggca tttaccatgt tgcccatatc gctgttgcag
    12901 ttgtcaacgg cttcggtatt cttaagtaca atattataag cgttcttaga gtcctcagag
    12961 ttctgagtta tctgctttga aacatcactg agggtagcgg aaagctcctc gattgcagaa
    13021 gcctgctgtg tagaaccctg tgatacagac tgtgcgatat ttgacacctg aaccgctcct
    13081 gagttcacct gctcggctga catattgata gaagcaaagg tatcagacag tgagtcgagt
    13141 atctcgttaa acgtactctt tatcttatag aagtcgccct tgaaagtgcc ttccattctg
    13201 tggcaaaggt taccctcaga tatctgggta agagccactg tgataaggtt gatatactgt
    13261 ctgaggcaga cgatagtttc ttccagagag tttgaaagga cgccaagctc gtcctttgaa
    13321 taccacacgt caacagggtc ggtaaggttg ccctgtgcga gagaacgaag tctgtttgtg
    13381 gtagaaatga taggctttgt tatcctcttt gcaaaaagca gagagaacag caccgtcagg
    13441 cacataagca caacgcccat tacaatgata ttagtaagtg cgctggagct tgcagtggtg
    13501 aagtcggcaa catttgtaac catgacgaca gtaccgtcaa aatatgacga accataatag
    13561 cccacgatat aattgttatc cttaaattta tactctgttg taccgctatt atttgcaagt
    13621 attttctcaa cggcatttgt cacttccgta tattcttcat ctttcttacc gatctcaagg
    13681 gcatttttac ctatctgatc tgtatcttta tgaagaagga tctctccgtc tttaccaaca
    13741 agataaacat agccggattt tccgaaggta tattcattaa gagcattgac aagcatatcg
    13801 ctctgaatgg taatacaagc gactttttgt tctgtgttgc cagactttgt tgaagcaagt
    13861 atagaaaaat aattcttacc gccaaaggta tatatatcgg ttatataagc cgagcttctt
    13921 tcgcaagcac ttatgatgtg ctccttttca actgatgacg ataccatttc attatctgtt
    13981 gcaagcacaa tgccattgct gtcaaaaacc gtaaaattca gatagaaacc tgtgttgtct
    14041 gcaaagccac tttttaccgc ctttagtctg tcagcgtcag tttttgcccc taaaaaagaa
    14101 tcagacttta cggtcgaaac tatattattc gtataagagc tgatcgtcga gtcaaaattc
    14161 ttagccgcct gaacagccag tggttcagcg gtgcttttga gcgtatcctt tgcagaaacg
    14221 tttataaaat acacggaaac caagctgagc agcaccgtga ttataagtgc gattgaaagc
    14281 attgccaaaa gcagctttga acgcaacgtt ttcatataca gttccccctt atgaacttgt
    14341 ttttaacgca gcacacaaca ctgcgatatt acgtgtttat tatatcataa ttgcacaatt
    14401 atttcaagta ccacaagcat attttttgtg aacttatgat gcaattaatg atatggaggt
    14461 gaataaaaat aggtaaaaag ggggtaaata aaatatgtac ataagtctaa ggataagaaa
    14521 aaaggtgatg ctttgcgtta tagctgtgct ttctatgctg gcggcggtgg ctgttatgcc
    14581 gacctttgca aaagaagaaa aaacagatgg gataaagctt ccgatcataa tgtatcacag
    14641 catcgtaaaa aacgaagatc gcagcggtga gtatgtcata acgcctatcg aacttgaaaa
    14701 ggatctgctt tatctgaagc agaacggcta cactactgtt tttgtaaatg acgttataag
    14761 atatgtgaag cgtggtggcg agctgcctga gaagcctatt atactgtcct ttgatgacgg
    14821 cacttacaat tacagagaat atcttctgcc gcttcttgaa aaatacgata tgaaagccac
    14881 tgtatctatt gtgggggctt acactgacgc tgcctgcgag gaggctgagc ctgatcctgc
    14941 ttactcttat cttgactggc aggacgtttc ggtgcttcgt gacagcggtc atgtggagat
    15001 atgcaatcac tcttacgata tgcacaatct tgaggggagg cgtggagttg gtcagctaga
    15061 gggggaaagc tacgaggatt accgaaagat atttcttaac gacaccacga agcttcaaac
    15121 gctttgcgat gaacactgtg gttttcagcc aaacgtttac acctatcctt tcggcataac
    15181 ctgcgaaagt gcaagtcggc tcgtaaagaa catggggttt gagtctagtc ttggagtaga
    15241 ggaaaaaatc aatataataa aaaaagaaga tgagcgctgc ctttttggtc tttacagata
    15301 caatcgcagc gggatcatct ccacagaaga attcatgaaa aaagtttttg gagcataaaa
    15361 aaaagcagcc cctattggag ccactctcta aactgaatca ccatcagcat attcacatta
    15421 tactctggca ggtgtcgaaa ttatacttgt ttttgtgtga tttttgtaaa aaaaatgtgt
    15481 attcgtgtaa acagggtgac aaatgctgtc acaaacagcc aaaagcacgt tgatacgtca
    15541 cttttggctg tt
//
LOCUS       DAWEMR010000046        15431 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1594_length_15431_cov_9.803200, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000046 DAWEMR010000000
VERSION     DAWEMR010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15431)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15431)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15431
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1594_length_15431_cov_9.803200"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2707..2716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4376 a         3243 c         3587 g         4215 t
ORIGIN      
        1 ctgttgacaa agccctgaga aatgtactat aatgagggtg aagttttgta cttgatacag
       61 aaaaatgtac aaaccaatgt gtgtaaaaat aaggacttat acataggcgt gccttgttgc
      121 ggtatagcct atatcttctg aggtgaacga gttgataaaa aagatgattt catatatcct
      181 gtcatttgct attgcagcgt caatggcggc ttcatgtttg acggtttcag cggcaaatat
      241 gatgggaagc tgtacggcag acgttctgaa cgttcgcagc ggtgcaggca caggctattc
      301 taaaacaggc acagtaagct atggcgacag cctgactata ctttccgaaa ccaccgactc
      361 aagcggtgca aaatggtata agatatcgtg cggcaatgtg acgggctatg tttctgctgc
      421 gtatgttcag ctaacatcaa gcggttcaca gggcagttct gacgctgatt ttgagagcta
      481 tatgacaaag cagggttttc ctgaaagcta taaaccatat ctgcgaaagc tccatgaaca
      541 gcacccaaag tggatattta ccgctcagaa gcttggcgtg gattggaaca ctgcgttaaa
      601 ggaagaatgt gttgtgggca gaaaccttgt ccattcttcg gcacttgcat cgtggaagtc
      661 tatggaaaag ggtgcatatg attttaacgg cggttattgg tatgggcttg acggctcatg
      721 ggtggcggct tcaaaagaga tcataatgta ttatatggat ccgagaaatt ttctcaacga
      781 tacatacatt ttcatgtttg aaaatcagtc atatgatccc tcgtatcaga cggaaagcgg
      841 tgtaaaaact attcttgcag acacttttat gagcggaagc tacacttgtc ctgacacaaa
      901 gaaaaagtat acatattcgc agacctttat ggacgctgca aagaaatcgg gcgtttcacc
      961 ttatcacctt gcttcaaggt gcaggaacga gcagggcgta aatggtgcgc cgcaatccct
     1021 tggtactgtg aaaggctatg agaattattt caacttcttt gacattcagg cttatgcgac
     1081 ctccaccatg acagccgcag aaatgggctg taagtatgca aaaactacta atccgaccta
     1141 tcttcttcct tggacaaatc agtacaagtc catagtgggc ggctcgatat tcttaggcac
     1201 aggctacata acaaagggtc aggataccct ttatctgcaa aaattcgata tggtagacgg
     1261 cggaaacggg ctttattacc accagtatat gacctgcgtt ttcggtcagg cgaatgaagc
     1321 cattagccta aaaaacgcct attctcagga tattttgaac agcgcaatgg agttcaagat
     1381 acctgtttac aacaatatgc ctgataaact ttgcccaaag cctactagca gtggagataa
     1441 caacaattat cttaaatctc tcagcgtttc aggcacgagt atttcaccaa aattcgataa
     1501 gttcaccgca agctatactg ccaaagtcaa tgcggaggtt tcttctgtta ctgttaatgc
     1561 aaatccgctg ggcaaaagtg caaaggtgtc tggaaagggc aaggtgagcc ttaaaaccgg
     1621 cgaaaacact gtcaaggtca cctgcacagc ggcaagcggt gtgaaacgga catacaccat
     1681 aaaaataaca agaaaagccg cttcacagac cttgcagcag ggagatgtga acggtgacaa
     1741 gtatctgaca gttgtggacg ctttgctcat gctgcgttac aatgcaggca agacacagct
     1801 tgattccgct cagctaaagc gtgcggatat gaacggtgat ggaaaggtcg atgttatcga
     1861 cgctcttaca ctgttgaaaa agataagtca gagttaaaaa tgcaaaaaaa caggagcgtt
     1921 accaccatgg tacgctcctg catgagggct atttcatcat cattgaaatg ccgtccatta
     1981 cgtcaccgat agctctgata gcacgacctg tgcggctttt gagcattttc ttttcgcttg
     2041 atgtcgcctc tgtgactgca taagccactg ttcctgcggc tatcgccatt gaaacgcctg
     2101 ttatcattga cttcatcttc attgctgttt cctccttttt tctttgtaat attatttttc
     2161 gcagtaagag aacagctatg ccatgcaaat gaagtcaaga ggactgacgg tattttcccg
     2221 tgaatgttcg tattttgtga tgatgtgaag gatatttcag ttgatgtgta gtgctattgt
     2281 acaaaatgtg attaaaattt ttgcaattta tatgtaaaag taaaagagat atgttcctat
     2341 ctcaaacgtt tagtataaat attattcata gatatataat aaatatgtat ttgcattaac
     2401 atcaaaatag tggtataata aatgggtata aaaaataaag gatcttggta aactctgaga
     2461 tctgatagta aggagtaata ttaaatgggt atgacaatga ctcagaaaat tctggcagcc
     2521 cacgcaggtc ttgacgaagt caaggcaggt cagcttataa tggcgaacct tgacctggtt
     2581 ctcggcaacg acgttacgtc ccctgtggct atcaacgagt tcaataaagc gggctttaca
     2641 gatgttttcg ataagaataa ggtcacaatg gtcatggatc actttgcacc taacaaggat
     2701 atcaaannnn nnnnnngtgc aagacctgcc gtgattttgc ttcaaagtat gacataaaga
     2761 attatttcga cgttggcgat atgggcattg aacacgctct gcttcctgaa aaaggacttg
     2821 tcgctccagg cgactgcgtt atcggtgctg actcacatac ttgtacatat ggtgcacttg
     2881 gcgcattctc aacaggtatc ggctcaacag atatgtgcgc aggcatggca aagggaaaga
     2941 cttggttcaa agtccctgcc gctatcaagt tcaatatcgt aggcaagctt ccaaagtata
     3001 gtgccgctaa ggacgttata cttcatataa tcggcatgat aggcgttgac ggcgcacttt
     3061 acaagtctat ggaattttca ggcgagggtc tgaaaaacct ttccatggag gacaggcttt
     3121 gcatggctaa catggctatc gaagcaggtg ctaagaacgg catatttgca gttgacgatg
     3181 ttacacttga ttacatcaag gataaggtag acagagagta taagatatac aaggctgacg
     3241 atgacgctga gtatgaggct gaatacacta ttgacctcag ccagataaag cctacagtcg
     3301 ctttcccgca tctgcctgag aacgcaagaa catttgacga tatcccggag gtcaagatag
     3361 atcaggtggt catcggctca tgtacgaacg gaagaataga ggatctgaga tgtgccgctg
     3421 agatacttga cggcaaaaag gtggcaaaga acgttagatg tatcgttatc cctgccacac
     3481 agaaggtata tatgcaggct atgaaagagg gtctgctgga gatattcata aatgcaggct
     3541 gtgcggtttc tacacctacc tgcggtccat gtcttggcgg acatatgggt attcttgcaa
     3601 agggcgaaag agccgttgca acaacaaaca gaaatttcgt gggacgtatg ggtcacccag
     3661 aatcagaggt ttatctggct tcaccttacg ttgccgccgc ttcagctgtg acaggcaaga
     3721 tctcacagcc gtcggaagtt atgtgatatt cggggagaaa atcagctgac agatcttgcg
     3781 ttgggggaat aattgatatg cggtatgacc gcatatacga cttgggagcg agaacagatg
     3841 aaaaattatc ttagggaaat attctccgat atacttctta gtattgttac aaagaagtac
     3901 ggaacttcac taaatgacta tcagcgtgag gagaaggctg acgagatcat tcaggaactc
     3961 catgacaaaa atacatttac cgtggaaatg acgcaggctc ttattgacaa gaagggcttc
     4021 aatacctttt atacctccaa tatcgggggt acacctgtgt atgctctcgt taaagaggga
     4081 atgttccaca aggtaaagat ctgctatttt attacaagaa acaaggatac tatagacggc
     4141 ccgtatcttg aaaagatata tgaagagctt agaaaacagg cgataggaga aaatatattc
     4201 cattcgtcag agttcaagca gggctgataa agtttgacga aaggggtttt cataatgaaa
     4261 gcttgcggaa aagtacataa atacggggat aacgtagaca cagacgttat tatccctgca
     4321 agatacctca acacacctga tatgcaggag cttgcacagc actgcatgga ggatattgac
     4381 gttgactttg caaagaacgt tcagaagggc gatataatgg tagcaaagag caatttcggc
     4441 tgcggttcat caagagagca tgcgcctgct gctatcaagg cgagcgggat atcctgcgtt
     4501 atagcaaaga cattcgcaag aatattctac agaaacgcta taaatatcgg acttcctatc
     4561 attgagtgcg aggaagctgc tgagaagata gataatggtg acgaggtgga gatagatttc
     4621 gacagtggcg ttatcacaaa caagaccaag aatgagacct atcagggtca gtcattccct
     4681 gagtttctta tcaatatcat caacagcaat ggtcttctta actcacttaa agaagacata
     4741 ttgaagctgt aagggggatc tctcaaaagg ggtcgccctt gaactgtttc tatgaagggg
     4801 tgaagttatg aaagctaaaa agctatttgc ggcacttgca gcaggggtaa tgctggtgtg
     4861 cagcgcaggc tgctcgaaaa aagaggagag cagtaataaa tttgaataca gcagcaagga
     4921 ttttctgcct tttatggtaa tggtggacgg cagcaagata gacctgaccc taagctctgc
     4981 agagataaac agaaagcttg gcggcagttt tgcaagggga actgcttcaa acagcagtat
     5041 agtgcttgtg gaaaaggacg aaaggaccga taaaaaaggc gttacaacaa agacctttgc
     5101 ctgcaataag cctgagataa atggtgctgg attttccact ttcaacggac tttgggcgaa
     5161 tatagacgaa gcaagcatag acagcactgt ttcacccaac agcataaagg tcaaaggctc
     5221 tgacggtttg tcttactact ggtcatatta tatggatggt gaggaaatag actatagtca
     5281 ggtgaacttt gacggcactg atacggcgga cagttatatg cagtatcagc tcaaaggcat
     5341 ggctgctgac gattattgca aaaagaagct tattgacggc gagtgttcgg tctatacagg
     5401 cattttgttc atgacagccg aaaagcagag ctattcctgc gagataagcg tttcgacttt
     5461 aaattctgag gaggaaaaat aacatggaaa agaaaataac agttataaag ggcgacggga
     5521 taggccctga aatagtaaca gaagcacaga aggtcctcga caaggttgct gaaaagttcg
     5581 gtcataagtt tgtttatact gatatcctta tgggcggctg ttctatagat aaatatggcg
     5641 ttccgcttac agacgaggct gtcgagatag caaagaactc agacgcagtt ctgcttggct
     5701 ctatcggcgg aaatacttcc acttcaccat ggtataagct tgctccgaac ctcaggcctg
     5761 aagccggact cctgaaaata agaaaagagc ttggtctgtt cgctaacctg cgtcctgcaa
     5821 atctttatcc cgagcttaga gaagcttgcc cccttaagga cgatataatc ggcgacggct
     5881 tcgacatgat gataatgaga gagcttacag gcggtctgta ttttggtgcg agaaagacag
     5941 agaacgttga cggcgttgaa actgctgttg atactcttac atataacgag aacgagataa
     6001 gaagaattgc tatccgtggc tttgacattg ccatgaagag aagaaagaaa gttacaagcg
     6061 ttgataaggc aaacgttctt gattcttcaa gactttggag aaaggtagta aacgaggtag
     6121 ctaaggatta tcctgaggtc gagcttgagc atatgctcgt tgacaactgc gcaatgcagc
     6181 ttgtaagaga tcctaagcag tttgacgtta tccttacaga gaatatgttc ggcgatatcc
     6241 tttcagacga ggctgcaatg atatcaggct cacttggtat gctggcttcc gcttcactga
     6301 acgacacaaa gttcggtatg tatgagccta gccacggctc tgcacctgat attgcaggac
     6361 aggataaggc taaccctatc gcaactatac tttcagcttc aatgcttctg agattttctt
     6421 tcgagatgga taaggaagca gacgcaatcg acaatgctgt caagaaagtc cttgaagcag
     6481 gctacagaac aggggatatc atgtcagagg gcatgaagct cgttggctgt aaggaaatgg
     6541 gcagtcttgt tgcagatatg atataacagc acgataccag ttttcagacg gtgataataa
     6601 atgtattatt atatttacgg aataacagac aagggaaatt accgtgaaca gaatgaggac
     6661 tgcattttaa tagaccacga ggtcatcaac agcggcagct atgaatcaac tgtcgctgct
     6721 ccttttattg cagccgtttg cgacggcgtt ggtggagaaa atgcaggaga ggtcgcctcg
     6781 gagctttgtt taaggcatct ttccattctt gaatataatt caggggtgga tatgaaaaga
     6841 acgcttatcg atgttcacaa caaaataaaa aagcagggcg taagggctga aaacgctgca
     6901 aatatgcaga ccactctttg ttgccttgcg gtggacgagg aaagcagagc aatgtgcata
     6961 aacgttggcg acagcagaat gtaccgttat gtgaacggca ctatcagaca gatatctgtt
     7021 gaccaatcct atgcacgata tatgtatgag catggcagga tagaagatgt gtcagagctt
     7081 gagctacagt atcagaatgc cataatatct tctatcggca gcactctcaa tgaacctgat
     7141 gtggcacaaa caccattggt ggctgatttt ggcaaagaac ctgacgatat gataatcata
     7201 gtgtcagacg gtgtgtcgga ttacgtttct gaggaagaaa tggtggtggg gcttggtctt
     7261 gacctttctg tttctgaaaa gctaggcgct ataatggagc ttgcccttac aaacggcgga
     7321 actgacaatg tttccgttgt gggcataaag ccttatcttg acgatcatga gcttaaaacg
     7381 ctcacagcaa aaaaggctgt ggaaaagaca gtgagcgtgc aggatatgct tgaaaataag
     7441 aagcctgaaa aggcggaagc tgtagaggaa aagaagcctg acgagcaggc aaagcttttc
     7501 tctgatacgg tggacgagct tatgagccaa atgaatgaaa gtattgactt tttaaagcag
     7561 atatagtata atcatatatt gatatatttt tatgatctta gatgtatttt gtaatttgga
     7621 ggaaataaaa aatgttgaaa gagttacgtg gctcagacgt tataattgaa tgtttgctgg
     7681 agcagggcgt cgatacagtg ttcggctatc cgggcggagc agttctaaac atatatgact
     7741 cgctgtataa atattctgat aggatacacc atgttatcac cgcccatgag caggctgcct
     7801 gccatgcggc tgacggatat tcaagaacaa caggcagaac gggcgttgtt atcgcaactt
     7861 caggtccagg tgctacgaac cttgtaacag gtatcgccac agcttatatg gattctatcc
     7921 ctatggtggc tatcacagga aacgtaacaa gagatctgct tggtcttgac tcttttcagg
     7981 aggtagatat ctgcgggata acaatgccta tcacaaagca taactacatt gtgaaagatc
     8041 ctgcggagct tgctgacatt atcagagaag ctttctatat tgcaaacgag ggcagaaaag
     8101 gtcctgttct tatagatata ccaaaggata tcacaggcgc acttatggtc tatgagaaga
     8161 aagagcctaa gaaggtagta aatcataatc ctgagggcat aaacgaggag atagctgctg
     8221 cggcagagct tataaagaat tctgagaagc cttttatcta cgcaggcggc ggagttgttt
     8281 ctgctgacgc aacagaagaa atggctgagt tcgcaagaaa agtggacgca cctgtttcat
     8341 tgtcacttat gggtcagtgc gcacttgaca atactaagga ctgctatatc ggtatgcttg
     8401 gtatgcacgg cacaaagaca gccgctatga cgctcagcga atgtgacctt atgatagttc
     8461 ttggctcgag attttctgac agagttcttt gcaacgctaa gacatttgca aaggataaga
     8521 agatcataca tattgatata gaccctgctg agataggcaa gaatatcgac gtttacagcc
     8581 atgttactgg agacgttaag tatatccttg caaagctttg cgagctgctt cctgatatga
     8641 agcacgaggc ttggatgaat actgttatgg attggaaaaa gcagtatgcg ctgagaatgg
     8701 tgcctatcga gagcgaggac gaggtgcttc cgcaggacgt tatcgaggag cttgacagac
     8761 ttacagacgg caaggctatc atctccacag aagtcggtca gcaccagatg tgggctgcac
     8821 agtattataa cttcaagtgt ccaagaagct ttgcttcaag cggcggactt ggtacaatgg
     8881 gctacggctt tggcgcagga atgggcgcaa aggtgggcaa ccctgacaga actgtagtaa
     8941 atattgcagg cgacggaagc ttcttcatga actgcaacga gctttcttca cttgcaaagc
     9001 ataagatacc gcttattgag cttgttttcg agaatgacgt tctcggaatg gtaagacagt
     9061 ggcaaagact tttctacggt aagagatttt ctcagactaa cattgaaaga ggaacagacc
     9121 ttatgaagct tgccgacgca tttggcgttg aaggcataag gataacaaag aaatccgaga
     9181 taaagccaat gcttgaaaag gcactcaaat gcggtaagcc ttgccttgtg gacgttatca
     9241 tcaacaagga tataaacgtt cttccaatgg tacctgccgg cgcagacgtt tcacagccga
     9301 taatgaatat cgaacttgat taagaaatcg aaatataggg ggtaataaaa tggtaaaata
     9361 tccaaatgcc gcaagcggta tcaaaaaggt gtttctggca aagatcatag gtgtgatagg
     9421 agttccgctc gcttttatac cggggatagg cgcacttctt ttgatgactt gtaccgtagc
     9481 cgcaactatt ttcaacgtat ggggaatact tgaagcttca aaagaccata atggttatca
     9541 gacagctctt gttttcacag tggctgatct tatcttttca atagttgata catttattga
     9601 caataatgcg atatcaagtg ttctgagttt tgctggcaat gtaatgaaca tagcggtgct
     9661 ttactatatc tgcaaatcaa caggcgacct gctcagggac gtgaagaact acgggcttgc
     9721 aaacaagggc gaccatgtgt ggaatgtaaa caagacctgc cttattgtca tggcagtgtg
     9781 ccttgttgtg gcatttatcc cgatactgaa cattctcgcc gcatttacaa cattggtggt
     9841 gcttgttgta actatctatg cgtctgtggt atatctgatc tatctctatc agagcggaca
     9901 gtatctcagc cttaacacta atttctaagc gatttgagag ctgtacggca aaggctgtac
     9961 agctcgtttt tgtaaggaga aactaatgaa atttacaaaa aaattcaaag aaaatgcagc
    10021 ccttgtggtt cttggcgtag tgcttttttg gggattgacc aactatgaca agttttttag
    10081 acttctgggc gtaatgatcg gggtcttgaa gcctgttata tttggctgta taatcgcatt
    10141 tatcctgaat gtgccaatgt caggcattga aaagaggctt tttaagaagc ctaaaaaaga
    10201 gaaatatgct aaaactgtgg aaacgctgaa aagaccatgc tcgatagtgt tgacctttct
    10261 gctttgccta ggcgttgtgg cacttgtgtg ctggcttatt atccctgcac tcataaagac
    10321 tttctctcag ctttcagacg atattcctgt gcttatttca aatatttcca acaagctcaa
    10381 caattcccct gagatagcag ggtggcttga cagaatgaat ataagcactg ctgatattat
    10441 cgataaggtt acaagctggc tgaaagacgg cgttgttata ctgaacacac tcagctccac
    10501 agtaagcttt gtgacagatc ttttctcagg tcttgtgaac ttcttcttgg gaatctgctt
    10561 tgcggtctat atgcttgccg caaaagagcg gctcaaagac ctgtgcaagc gcataagctg
    10621 tgcttttctc agcaatcgta tcaccgaccg cataagccgt atatgccgtc gctctatcga
    10681 cacttttgcg aactttcttg tgggtcagac cacggaagcc attatcctcg gctccctttg
    10741 cggtattggt atggctatct tccgtttccc gaatgcagtg cttattgcca tactcgttgc
    10801 ctgcactgca cttatcccta ttgtgggagc ttttcttggc tatgtggtgg gcttcctgct
    10861 tatatgcgtg acggatttca agcaggctgt gctctttttg ttgttcatgt tcattattca
    10921 ggctattgag ggcaatctta tctatccaaa ggtggtagga aactcggttg gtctgccgtc
    10981 actatggaca ctttttgcta tcaccatagg cggaaacctc tttggcatat tcggaatgtt
    11041 catcgctgtt cctgtgttct ctgttatata ctgcactctc ggagaggtag tcaactacag
    11101 aaatgaaaag agagctgtca aggtggagga catatcataa taaaatagtc cgtacaaaaa
    11161 aggctgcaca gtttcgtgca gcctttcgct atttatgcta ttttgttatt ttacatagct
    11221 tacatgaagc agttcatgct ctctgccctt gaggataccc tcgtaaagct tatccataag
    11281 cggattttct tctgaacgct tcaaagtgca aagcttgtca gcattgtaaa gattaacact
    11341 gcgctctttc ttctcgtcga tagtagcctt tggctgacca gcgccgccta tacagccgtt
    11401 tggacaagcc atgacttcga taaggtcgaa atgctcgcca gctttcattc tgttcattat
    11461 gacttcaaca ttgcgaagtc cgtttacaac gcagaccttg atagccttgt cgccaagagg
    11521 tacttcaagc tcctttatgc cctcgaagcc tcttacgccc tcgaatgcaa gatttctcag
    11581 ggaagcgagt gacttgtctg tggaaacacg tctgataact gcttctgtta caccgcctgt
    11641 tacaccgaag ataacgcctg cgcctgtcat agtgccgaac ggcatatcaa cagcttccgg
    11701 ctctatttcg ttgaacacga taccggcctc ttttatcatc atgataagct cctgagttgt
    11761 gatgacaaaa tcaaccagtg gtttgccgtt ctcgtccttg aactcatctc ttgcggcttc
    11821 gccctttttg gcagtgcaag gcataacagc aacagaaagt atcttcttgc cctcgccctt
    11881 gaacttctcg tttattacgg aagcaaacat ctgcattggt gagcggcaag tggaaatgtt
    11941 aggcagtatc tcaggatact cgttctcagc atactttatc catgcaggac agcatgatgt
    12001 gaacagcggg agctttccgc cgtttgcaag cctgtctgca aactcctttg actcctccat
    12061 aacagtgagg tctgcggctg tagatgtgtc aaaaatgtag ttgaagccta ttcttctaag
    12121 tgctgcaact atcttgccca taacgttctc accattgtag ccgaactctt ttgccactgc
    12181 aactcttaca gccggagcta cctgagccac tgcaatagtg tcctcctgat taagctcttt
    12241 ccaaagcctg tggctgtcgt tctttatgat tatagcgcct gttggacaga ctgccgcaca
    12301 ctgaccgcag ccaacgcagt ttgaatcgcc tataggcttg tcaaatgagc aggctacctt
    12361 catttttgag cctctgaatg caaaatctat tgcaccaaca ttctgtacct cgttgcaaac
    12421 tcttacgcag tcaccgcaga ggatacactt gttggcgtcc ctagttatag agacagaaga
    12481 atcatcgata caaggcttgg actttggatt gtcaaaacgt accctgtgga tatcaaatct
    12541 tgcggcaagc tcctgcaatt tgcacttgcc gtttttatcg caggtcgtac attcgccgca
    12601 gtgatttgaa agaagaagtt ccaatatcat cttgcggtat tttctcagcc ttggtgtgtt
    12661 tgtgtatatc tccataccgt ttctcggagg agttgagcaa gcggcttcca tgccacccca
    12721 cttgttttcc accatgcaca ttctgcaggc accgtatact gaaagctcag aatagtaaca
    12781 gaatgtagga agctcgatgc ctgcctttct gataagctca agcagatttt tttcaccgtt
    12841 tatagcaacg gggatattgt cgattatcat aaactcttta ttcatagcta aaattaaacc
    12901 tccttgacag ctttgaaatg gcagttggaa atacaagctc cgcactttat gcacttagag
    12961 gtgtcgatct tgaacggctt tttgatctca ccgctgatcg cacccacagg acagactctt
    13021 gaacactttg aacagccctt gcatatctca gggtcgatct gatatgtcac aagtttcttg
    13081 cagttgcctg atggacactt cttatctctt atgtgagctt catattcctc acggaagttc
    13141 tttattgtag atacaacagg gaatgcagcc gtctttccaa gaccgcagag agcagttgag
    13201 gagatagtgt cggcaagctc aagaagcagt tcgatatccc catcctgacc gttaccattt
    13261 acgatacgct caagtatctc aagcattctc tttgtaccct cacggcacgg aacgcacttt
    13321 ccgcaggact cgttctgggt gaaattcatg aaaaatcttg caacttctac catacaggac
    13381 ttatcgtcca taacaacaag tccgcctgag ccgatcatag caccgacttt tttcagcgag
    13441 tcaaagtcaa gctggatatc gaggttaggc gtaacaagac agccgcctga cggaccgcct
    13501 atctgaacag ccttgaactt tccgtcgcct ctcatgccgc cgccgatatc gaatattact
    13561 tttctgagag aagttcccat tggcacttca ataagaccgg tgttcgagat gttgcccgta
    13621 agagcgaaag cctttgttcc aggggagttc tcagggccta tgctcctgaa ccattttgca
    13681 ccgttgttga tgataacagg cacgttagcc agcgtttcaa cgttgttgag aactgtaggc
    13741 tcgttgaaaa gaccatgttc aacagttctt ggcggcttaa ctctaggcat acctctcttg
    13801 ccctcgatag aagccgtcag ggcagagcct tcaccgcaaa caaatgcgcc tgcaccacgg
    13861 tttatatgta ttctgaatga gtaatctgtg ccgagtatat tgtcaccaag cagaccaaac
    13921 tgctcagcct gtgcgatagc acctttaaga cgtgatactg ccagcggata ttctgcacga
    13981 acgtagatat atccctcttt tgcgcctgtt gcgataccgg ctatcatcat gccttcaaga
    14041 agtctgtgtg ggtcgccctc catgatagaa cgatccataa atgcgccagg gtcgccctcg
    14101 tcaccattgc agactatgta ttttgtaggg cttttctgct ttgcaacgct tgtccacttt
    14161 ctgccaagag ggaatcctcc gcctccacgt ccacgaagat tagattctgt catttcgttg
    14221 aggatatcct caggtgacat atcgagaagt gccttttcaa aggctctgta gccgcctata
    14281 gcaagatatt cctttatgga agtagcgtca atgtgaccgc agtgttcaag tacgatacgc
    14341 tgctgcatct tatagaatgg tatttctgac tgctggcgat atatttcgcc gtttttcttg
    14401 tagcacagtc tttcaacgca ctcgccattg cagatagtct tgtcaactat ctcgtcaaca
    14461 tcgtcaagct ttaccttagt gtaaagccag ccctccggct ctattctaac aagtggaccc
    14521 atttcgcaga agccgtgaca gccgcttctt tttacgccga tagcgccatc gtggatatca
    14581 tcatcatgtg gctcgtcagc aagtgctacc acgcactcca atccctttgc ggagatagtt
    14641 tcgataagct tctggtatat gttcagcgaa ccgcctgcaa cacagcctgt acctgcgcaa
    14701 acaagcactt ttctagcctg cttgtcaaca agcttaccat actgttcacg ggcgttgtca
    14761 agatcgtatt tcgttttcag cagcatatta ttcagcctcc ttttctctca gttccttaag
    14821 cagagccgca gccttgtcag gattcatctc agggtgaaca acatcattca cagtgagaac
    14881 aggggcaagt ccacaagcac caagacatga aacagtttcc actgtgaaca tcagatcgtc
    14941 agtggtggtt ttcttctcgc taagaccgag ctctcctctg agatattcga gaataggtat
    15001 agattttctt acatgacagg cagtgccgtc acaacatttg ataacgtatt tgccctttgg
    15061 ctcgagagaa aaattctcat aaaaggtcgc aacgctgtat attcttgctt cgcctgtgcc
    15121 aagctttttt gcaagatatg ggaatatttc ctttggaaga tagtgatata tctcctgaat
    15181 atcctgcaat atggctatga ccgctgtttt ttcatgattt tcaagaattt tgtcgatcgg
    15241 tgaataatca aattcttttg tcataaaatt tcctccatat aattaaagtt aaatttgatt
    15301 acaaagtcaa taatcaagac atactgtcaa ctataattat atactatatg acacaaaatg
    15361 aataatgttt ttatttacat gggctatata taattagaat aagtattgac taacttgtag
    15421 aaatacacat a
//
LOCUS       DAWEMR010000047        15386 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1596_length_15386_cov_13.428870,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000047 DAWEMR010000000
VERSION     DAWEMR010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15386)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15386)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15386
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1596_length_15386_cov_13.428870"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4258 a         3558 c         3072 g         4498 t
ORIGIN      
        1 cccttttttc gctttcggct ttttgtacac tttagttcac cttttttcgt tctttttgga
       61 taggttgtcg aagataacga caaaagcggt gcagatttta tactctgaca ccgcttttcg
      121 ctcttgatac ctctttattt attatctctc attattttaa ctgtatccga cataagatct
      181 gccgctttct gctttttctc caacgtcacc gcaaaatacg gcttatctcc gtcaaagaaa
      241 cgtattctat tcttataagc tccatacaac gcttgtatct gctccacctc tgcgcttttc
      301 acaaagaaca gcaccttatc ccctctttgt gttatttctt ttatgccaag ctttgaagca
      361 gtatttctcg taagtgcaac gttcaccaat cccagcactg atttcggtgg gtcgccgtat
      421 ctatctataa gctcgtcgat aacgtccctg gaatcctcct cattgcctat ggaagctatc
      481 ttcctatagc aatctatcct ctgtgacaga ttttctatat aatggtcagg tataaatgcg
      541 tctatggata tatccaccaa acaatcctca ggtgacggtg gcagagcctc gcccttttgt
      601 tcagctatag cctcatttag aagtcgcaga tacatatcat atcccaccgc ttccatatga
      661 ccatgctgtt taccgctgag tatagagcct gcacctctta tttcaaggtc acgcaatgct
      721 atcctgaagc ctgagccgaa ctgtgtgaac tctcttattg cgtctagcct ttttgccgct
      781 acctccgtga gtaccttgcc acgtctgaaa gtgaagtacg caaaagcacg gcggtttgat
      841 cttcccacac gtcctctaag ctgataaagc tgtgaaagtc ctaatctgtc agcgtcctct
      901 attataagtg tatttacatt cggcacgtca acacctgttt cgattattgt ggtgcagaca
      961 aggatatcta tttctctgtc gcaaagctgc cgccatattt ccgacagctc ctcctctggc
     1021 atttgtccgt gtgcgaacgc tatccttgcg tcagggagca tttcctgaag cttatttgcg
     1081 gtcatcatga tagtttccac cctgttgtgt atataataca cctgaccgcc acgtcgcagc
     1141 tccttgctta tggcctgcgc tataacaccc atattatgct ctataacata ggtctgaaca
     1201 gggtgtctgt ccataggcgg ttcctctatg accgacatat ctctgatacc gctcattgcc
     1261 atgttaagcg ttcttggtat cggggtagcc gaaagtgtaa gcacatcaac acccttgaaa
     1321 gtttccttga acttttcctt atgggcaaca ccaaaacgct gttcctcgtc tatgataaca
     1381 agacccaagt ccttgaactt aacgtctttc tgcacaagcc tgtgtgtgcc tatgataagg
     1441 tcgatagtgc cacggttaag ctctcttatt atctgctgtt gctcctttgg ttttctaaat
     1501 ctcgacaaaa gctccacctt cactgggaaa tgctcaaatc tttttatcgc cgtctgataa
     1561 tgctgccatg caagaacagt tgttggcaca agtatagcga cctgtttgtt gtcaagcata
     1621 cacttgaacg ccgcgcggag agccacttcc gttttgccga agcccacatc tccgcagaga
     1681 agtctgtcca ttggggacgg cttttccata tctgacttta tctcctgtat agatcttaac
     1741 tgatcgtcgg tttcctgata gtcgaaacgc tcctcaaagt ccgcctgcca atcgttgtca
     1801 ggggaaaaag caaaaccctt tgccatttgt ctttgagcat aaagctttgt aagctcctcc
     1861 gccatatcct ttacggcaga ctttaccttg tttcgtgtct ttgtccactc catagagttg
     1921 agcttattaa gtttcactgc cccgtcatct ctaggaccta tatatcttga cacaaggtca
     1981 agctgagtaa cgggcacata aagcacgtcc gtgcctgcat attttatggt gatataatcc
     2041 tttgttacac cgttcgtttc tatatttttt attcctgaaa atctgcctat gccgtacata
     2101 gcgtgaacaa caaggtcgcc tgtctgtata tccgaaaggc tttttatctc ttctcctgcc
     2161 ttgtgactgg atttacgctt ctttgcactc atggctttca tctgagtgat agccgcacag
     2221 cctatgtcag gataatcaaa gccacctgac agacagcccg tgcgcaggta tactttaccc
     2281 tttattatct cgctgtcctc agtgaggatc tccgcagaga tgccagcatc attaaggtct
     2341 ttatttataa taggaagcgt cttttccgag cctgccagca caacagtgca atagccctga
     2401 gagcagagat ttttcaattc ttcttcaaga ttgatgatat taccgcccca agccgccgtt
     2461 tgtcggcagt cggtattgat aagctttttg aacttcacgc cgttgttaga gcgcataaag
     2521 ctgtcaagaa acaccgactt catttgtgtt atttttagtt ccgcttgtgg tatatcaaga
     2581 taaaagccct ccagctttct gaaaagtatg ccgtcctcat aaagaagctt catatcctct
     2641 gaaagctgag caagagccgc cttgcccttt tcgatacagt tggtatactc cgacacaagg
     2701 caaacaccgc tttgaaaata gtcaaaaata gttgtgagcc tgtcatagca cacagtataa
     2761 tacttatcaa gatttgtgag cgtgataccg ctctgagccg tttctatatc ccttgaaata
     2821 ttcgttctta tcttatcggt acggcttcca cgaacagact tcgacaaggc ttcaagcttt
     2881 gtcacaaggt catcagtgtc agggaaaatt atctccaagg cagggaaaac cattatctcg
     2941 tctattgggt cggtacgtct ttggctttcg gggtcaaagt atgatataga gtcaacctcg
     3001 tcgccccaaa gctctattct cacaggacgt gactcctgca cagggaaaat atcggcgata
     3061 gagcctctca cagaaaactg cgcctgaccc tccacctgat ctgttcttgt atatccgcct
     3121 gcaagaagct tgcttgtaag ctcctcaagg ctcagttcgc cccctgtttt tatctcaaag
     3181 gaaaactctt tcagcttatc ctgcggaata gttccctgca agcaagcttc catacttgcg
     3241 gcgattatct tgcatttgcc ctgagtgata agtgagagag cttccagcct tttatgctca
     3301 tattctcttg aaacgccctc catgtatgca aagttcagat cctttgcagg aaaaacgcag
     3361 gcaagctgtt ccccagccat ttcgtttata tcccctgtca ttctaatggc agacgcttcg
     3421 tcgtcgcata taacaaggca aggggaaagc ctgctgagag ccattgcggt ctgagccttg
     3481 tgtatatttg aaacgcccgt gaccgcacaa ggggtaaacc ctttgagcag gcagtttttc
     3541 acatctttat aaaagccaag acggctcgcc atttcataaa aaatattcat cttgctgttc
     3601 tccaaaaaaa tatgcttaat tgaacttgtt catagcctcg tttatcttgc cctgcaccat
     3661 aagctccaca gcctcgcagg tgtttttatc cacatcagaa agctttttca tttcctcgtc
     3721 agtgaatctt gaaagcaccc agtcagccag gttgtaatca gggtgaggct ttttgcccac
     3781 gcctattttt acccttggga aatcctgacc cccgcacagt gcgataatgc tttttatgcc
     3841 attgtgaccg ccagccgaac cgtttctgcg tatcctcatt ttgcccacgt caagtgaaat
     3901 atcgtcatag accacaacaa cattttcaag cggtatatca taaaagtcca tagcgtcgct
     3961 gacagcctca ccgctattgt tcatgaaagt ttcaggcttc atgacaagac agctttttcc
     4021 gcctataacg gcacgtccca caagagcctt gaacttatgt gtattcaccg aaaagccgta
     4081 tttcttttcc atagccgcca ccgcaagaaa gcctgcgtta tggcgtgtat tctcataagc
     4141 cacaccaggg tttccaagac ccacgataat atactcaggc ttgcccatag ccgtttccct
     4201 ctcggcagac aatttcttca atttctcaaa tatatccatt gtaaattctc ctatctttta
     4261 taatatgtca gccccgacag cttattctat ccacgcacaa taaccccgtc tttatgcaag
     4321 acggggtata aacaaaagcc gacattttac tgtcggcttt attatagcat aattttttca
     4381 ggattgcaag ggagaattgc ttatatcttt atttagctgt cttttcttta taaaaagcca
     4441 tattgaacct acaattatta taattggaat aatataataa caataatgtg caacgcccca
     4501 aacataattt tcattctgca caacgtgtct tgcactaaga tcccaattaa aaataccaac
     4561 ggttatcacc gtaaaaatcg ttgatattat gatacctata ggcttttttc gaatagcttt
     4621 tattaccgct atcaaagaaa ataagcctat agcagcaatt agacctgcat agatccaacc
     4681 agcatactca tttttccaaa agttttcccc gacagcaacg tatattctcg cataatcaac
     4741 aaggcttaca ttttttacat ctgcacaagt caagctaaga cctttatata cttctgtatc
     4801 agcaaaccta tcaagccttt cagcatattc gtcagtagct gtagtaaaag gcaatacaag
     4861 caacaaaaac atcattattg tgcctatcaa agttataaca tatggtataa acagtttgtt
     4921 tttttctttc ataataaacc ctcatttttt ccttctgaac tttttatgat attgattgtg
     4981 atacttcaag taatttcttt gatatttttg cagacgcatc aatataatac gctgtttctg
     5041 catcgttcat ttcttctgat tcaagtttac tgaacttttc cattgtttca gaatattttt
     5101 tcatataatc cgtgtaatca ttaagcatag atatagcgtc attagaatta ttgtattttt
     5161 tcataaattc agcatactca tcaaaaaatt caacataact atcaatggct tccttgaact
     5221 caggtcttat taaatctgtt ttttcagact tactatcctt ttcctcatca gacccaattt
     5281 tcaaatcatt cttatcatct gttgccttgc ttgttatcat ctttgtctgt aattagaact
     5341 atcaaagccg ctaagatagc caacccaata atgataatta taatgcctcc acagccaatg
     5401 ccttttttct ctttttttct catgattgtt atacctccgc acttttaaga ccatacggtt
     5461 ttaatatgta tagcatagca caaaaatatg gtaaaatcaa tagtaccaat aggtactaaa
     5521 atcactttct acaatttata caaaaaacaa ggagaatttt tatgtatttc cagagattaa
     5581 gggatctaag agaagatagc gacatgaacc aaacgcagat agccgaaatt ctttttacaa
     5641 gtcagacagt gtattcaaga tatgagcgtg gtgcaagaac tatccccgtg gaacacctcc
     5701 ttattctagc ggacttctat ggcgtttcca ccgactatat cctcggcagg acaaacaaca
     5761 aaaagctaaa caaataaaag accctgcctt acaaaagaca gggtcttgct tatatttggg
     5821 tatattactt ctcctcagac ttgccgtgga tagcctcgaa cttttctgtt ctcgccttga
     5881 gcttcttctc agcgtagccc tctttgatcg tctctctgcc acgctcgata gcaagtgcgt
     5941 tgtcagggat atcagttgtg atagtagagc ctgccgctgt gtatgcgccc ttgccgactc
     6001 ttacaggtgc aacaaggttt gtgttacagc cgatgaaagc attgtcctcg ataacagttc
     6061 tgaacttctt gtcgccgttg tagtttactg ttacaacgcc acagccgaag tttacatttg
     6121 agccgacgtc agaatcgccg atgtatgtaa gatgtgccac cgctgtgtat tcgccgatag
     6181 ttgagttctt tatctccaca aagtcgccta tcttcgcacc cttcttgatg tgtgaattag
     6241 gtctgagctg aacataaggt cctatcttaa cgtcgtcctc gataacggac tcgtaagcct
     6301 gaacattgtt gaggtttacg ccgtttccta cagttgtgtt ctctattatg cagttgttgc
     6361 cgatattgca gttttcaccg atagtggtgt ttcctctgag tataactccg ctgtgtatct
     6421 ttgttccatg accgatagta acattgtttc cgatagaaac accgtctgtg cagcagaact
     6481 cgataccctc gtcaagccac tttcctatta tctcatatct tgctatatca ttgagtgcaa
     6541 gcagaccacg tctgtcattt gcaccaagcg caacatgagg atttgctgaa ataaatgcgt
     6601 cagccttttt gccctttgca ataagcagct cgatgcagtc tgtaagataa tactcgccct
     6661 gagcgttctc aggcttgatg tcgaaaagaa cttccagcag atcagccgtc ttgaaccagt
     6721 aacagcctga gtttatctca gtgatagcaa gctgcatagg tgtgcagtcc ttgtgctcaa
     6781 ctattcctga aatgccattc tcagtgcgga ttattctgcc gtagcctgta gggttctcaa
     6841 ctcttgatgt aacaacagtt acgccgcagt ccttttcagt atgaagctcc agcgcaccct
     6901 tgatagtttc tgcgtcgata aaaggagcgt cgccgtagag tataagtgtg tttccctcag
     6961 cgtgagcctt aagaaaatca gccgcctgca taacagcatg acctgtgcca agtcgctctt
     7021 cctgcataac agtgcagatt ttagcctttg actttctctc tgcaagataa tcctctatct
     7081 gctgaccctc gtagcctttt acaacgcaga tatcgtcaag ctctgcattt tcacaggctg
     7141 tcagcaccca ctccagcata ggctcaccga taacattgca caaagctttt ggcatattgg
     7201 ttttcattct cttgccctgt ccgccggcaa gaataataac attgttctcc ataatacaaa
     7261 ttcctttcag atacgtccca caaaataaga cttggcactt tcgcaccatt tccttattat
     7321 tataatacgg ttgggataaa aagtcaacag ttggttcata ttttatcgca atttattctt
     7381 ttgcttgaca acatattgcg acactgctat aataaaatgt gtagttatca tgttaatata
     7441 ttgcagtttt tttctgcttc agatatgggg gaaaaatata tgcagaaaca attctatcct
     7501 gttataggaa ctgaaaaagc tctgccattt tatatcatcg gcataggcgt gagctgctgg
     7561 cagtttcctg taagcagacc ggagggctat gattatcctc agcttttcgt ctgtcttgag
     7621 ggcgagggtg aggtaacggt agacggcaag accgtaaaaa taatgcctga cagcattttc
     7681 tatataccac cgcactgtcc ccacgaatat agagccatga ctcattcatg gtatcttgac
     7741 tgggtgtgct ttgacggcaa acaggctctc gaactgctga aagaatggga tcttaacaaa
     7801 tttcatgttt tcttaaactg tggcgcagag cgtatgcaca agctttttga cagcgcatat
     7861 tacaccataa aaagcgacaa ggcatacggc aactattatg cctccgcaca gctttacgat
     7921 atgcttcttg aataccgcaa gctttcagac gataagttcc catacaagag ccgtgtaaat
     7981 tccgaagccg tcgcaaaggt catgaaatac accgaggaga attattcaaa gcctataaag
     8041 ctcgcagata tggcggctgt ggcaggggta tcagaacagc acctttgcag acttttcaag
     8101 aaaaatttca acataagacc aatggaatat ttaaacacag tgcgcataag ccacgcaaag
     8161 gagcttctta cctactcctc aaagacagtt gcggagatag cttcggaaac aggcttcagc
     8221 gacagctcgt atttttatgt tgtattcaaa aagcatgaga acatctctcc tgctgaatac
     8281 aggaagataa aggcataagg tggcaataaa tatggaacaa aaatatatct attccttttc
     8341 tcagatagag gataaggtca aagctttgca tgaaaccaat actgaggctg cagcagccgt
     8401 tgccctttca tggctgggtc tgagccgtga tgagcttaaa acactcaggg tatcagacta
     8461 caacagcaca atgcgaatgc tccactctgc aggcagagtg gtgtacgtcc gtgaagatct
     8521 tatagcagat atgcttaaag ctatatgcga ggaggctcct gaatacagca tgggcttttt
     8581 catcacagac gtgaactctg catacgaagc cgcagaaaag caggagcttt cccctatcag
     8641 cgtgcttaaa atgagatact tctacgaaaa gcacagtgca aagctttcag gagagcctga
     8701 ggacagagta tttctcaaag agatactgcg ctcagttggc gaaagcgaag ctgatatgga
     8761 aaagcaattc gccgaatacg aaaaggacgc acctgaaaaa atatagggca ttaaataaat
     8821 tagatactgt aaaatcacat cataaaaacg ggcgaacgct gttcgcccct acgatttgcg
     8881 taaatacgct gtttatatgt aggggcgacc tgaggtcgcc cgttgatttt ttatacacca
     8941 acaaaaaaga gcaaaaccga agttctgctc ttttgtaaac tattctatta gtctatcaga
     9001 tcgaaattag atagaagaat ggctctttac atagtcaaca acagcgtcaa cagttgtgta
     9061 agcaagacct acgcaagtgt cagcacagcc gtagtaaata gcaattctgc ctgtctcagc
     9121 gtcgcaaagt gttgcgcatg ggaatgttac gttctgaaca tcgcctacgc actcatagat
     9181 ctctcttggg ttgatgagat actcgccgca tctgtactta accttccatg gctgctcctt
     9241 gtcaaggatg caagctgaga agctgtatac aaagccgttg caagactcaa gaacaccgtg
     9301 atagaagcag agccagccct cgtctgtctc tattggaata ggacctgcac cgatcttctt
     9361 ggactcccag cccttgagag gtcccataac gtgtctgtgc tcgccccagt acttcatgtc
     9421 aggtgactgt gaaatgtaca tatcaccgaa tggtgtgtga cctgagtcag aaggtcttga
     9481 gaacattaca tacttgccgt cgatctttct tgggaagagt acgccgtttc tgttgaatgg
     9541 gaggaatgcg ttctcgcact gatagaatgt cttgaaatca tatgtatagc ctacgccgat
     9601 agttggcttc cagccgtatg cattacacca tgttacccaa tatctgtctt cgataaagca
     9661 tactcttggg tcatagccgt agccccactg atttacttct tctgtagact tttcagcctg
     9721 ctcgaacatg atccttgttg ggttgatgtt ccagtgaata ccgtcctcag agaaaccagc
     9781 atggagagcc atgtttcttt ccttatcgtc aacacggaat acgcctgcga acttgccctc
     9841 gaaaggaact accgcactgt tgaagataga gtttgatgta ggaagaagat ttctaggaat
     9901 gatagggttt ctgttatctc tccagatcac ttcattgcag tcagccggct tatcctgcca
     9961 aggcatattt ggaatagcct gtccgttgat tactttaacg ctcatgttaa aatccacctt
    10021 tcataatata aaaaaatact gaggctgacc gtcaggtcat gcccttgttt ttattcttcc
    10081 cccgaataaa aatgcgaaac ttttcccgct tattcaaggc gatatcattc ggcttacctt
    10141 atcggtaaaa tgccgctgtt attattaaaa cacactttca gaaaaatttc aagcccctat
    10201 ttttgctgaa aacgtttaaa atttatatgt aaacgtattc ttcttaaatt taacatttta
    10261 ttaacagttt gcttggtttc atatttttgc atcgcaataa aaaactgcga ctttatccgc
    10321 ctgcacgacc gttctccgcc caatccatga taaaaatgaa atatcaggca gtattttgaa
    10381 tgtaaattat aaaagttgcc cataatggtc atattagcat aagcatattc gtgcaagttt
    10441 ctgatttttt gcaggaatga taaaaagcgc ttgcaaaaac agtgaaaaag gtatacaata
    10501 ttaaataagg atatcggata cgttcggcag agtgcggcga aagtcgggat ttgccgaatt
    10561 gttctgtata gtagtgtact ataatttaca atttacaagt ggaggacatt gatatgaaac
    10621 taacagaaat gtctgcagag gaactggcat cattcaaagc agaagtccag aaaaaatatg
    10681 acgatttcaa ggctcaggga ttgtcgctga acatggcaag aggaaagccg tctgccgaac
    10741 agctcgacct gacagagggt ctgctcacag cagtaaagag cagcgaggat tgtttcgctg
    10801 aagacggcac agattgcaga aactacggtg gtcttgacgg tctgcctgag gctaaggcac
    10861 ttttcgcacc aatgcttggc gtaacgcctg atgagcttat catttgcgga aactcaagcc
    10921 ttaacatcat gtatgacact atcgcaaagt tcatgctttt cggcacaggc gacggtgaga
    10981 agccttggaa ggacacaaaa gtcaagtggc tctgccctgt tccgggatat gacagacatt
    11041 ttcttataac agaaaaaatg ggctttgagc ttgtaaacat tccaatggac aaaaacggtc
    11101 ctgacatgga tatggtagaa aagctcgttg ctgaggacga tactataaag ggtatatggt
    11161 gtgttccaat gtatgctaac ccaacaggca caacatactc tgacgaagta gtaaagcgtc
    11221 ttgcagccat gacaacaaag gctaaggact tcaagatact ctgggataac gcatattgta
    11281 tccaccacct ttctgatacg cctgacacac ttctcaacat tcttgacgag tgcgaaaagg
    11341 caggcaaccc tgacagagtt tatatgctcg catcaacatc aaaggtaaca ttccctggtg
    11401 caggcgttgc aatgatagcg tcatctgaaa agaacatcaa gtacctcaaa tctatgatga
    11461 cagttcagac tatcggttat gacaagataa atcagctccg ccacgttaag ttcttcggca
    11521 catatgaaaa ccttatggag catatgaaga agcacagagc tataatcgag cctaagttca
    11581 agatagttct tgacactctc gaaaaggaga tagcacctct gggaatcgga agctgggaga
    11641 agccaaacgg tggttacttt ataagcttca acgctcttaa cggctgtgca aagagaattt
    11701 gtcagctttg caaagaggca ggcgtagtcc tcacaggtgc aggtgcaaca tacccttacg
    11761 gaaaggaccc tgacgattca aacatcagaa ttgctcctac attcccgcct gagagcgagc
    11821 ttaaagttgc agccgacgtg tttgctacag cagttaagct tgcaagtgca gaaaagcttc
    11881 ttgcagaata attcaactaa gtcaagataa caatactctc tatataaaaa agcgatgacc
    11941 ttgcaaaagg ttcatcgctt tttttgtttg cctataaagc aaaagcggta tcgacctcct
    12001 aaaaggtcat accgcccgct atggtaagga aattaaaatg ggaataaagc ttattctttt
    12061 acagctcccg aaacgagtcc gctgtagatg tacttttgca gtaagaggaa tataataaga
    12121 gtcggaatga tacaaattac gattcccgca aaaactacct cccactgtac agaagtggca
    12181 ttcataagat tgtaaagcac agttgagata acaacgtggt catcatcagg catataaagc
    12241 tttggagtgt agaaatcgtt gtagatacca acgaacttga tgataagcac tgttgctatt
    12301 gcaggcttta ggttagggag aattatgctg aaatataccc ttggataaga agcaccgtcc
    12361 ataatagcac tttcgtccag ggaatatgat atctgtccca aaaactgaat gaaaatgtag
    12421 atagaaatga tatcagtacc gatattcaaa atgataggtg caagcataga tctgtaaaga
    12481 ccaagcttgt aaactatctg gaataccgtt acctgcattg tgatattagg cagaagcgtt
    12541 gcaataagga acgctgtctt tactgttctt gaaaaatagg tgttgaaacg ctgaacgaca
    12601 taagccgtca tcgtacctgt tattatcgaa ccaacgcagg atacaacaat gattatagcc
    12661 gtgtttttga atgcaagaag aatgttctgt gtaaaaaacg ctgtcttgaa gttctcccac
    12721 tgaggcactt ccggaagagt gaacaccgtg gtgtttgaaa gctctttgta ggttttcagc
    12781 gagcctatga gcacaacaag tatcggtact attactataa gacagcagag tataagaaaa
    12841 gcatacttga aaatgttcag tacccttgag ccgatataaa gcggctttga atctttgttt
    12901 tcattctgtg ccattatttc acagccccct ttctgctcct tttgttctcc ttaggggtct
    12961 tgtcgtcctc acggaagaag tatttttgta taaacgttac gattatcgtt atgataagca
    13021 gcacgatacc cattgccgaa gcaagaccca cctttttcag cgagaacgcc gtgttgatag
    13081 tcttgataac gaatgtggta gtgcccatca taccctttgt tgtgatatat ggtatctcga
    13141 atactgagat agcacccttt acagcaagga taagctgcaa tgagatgata ggctttatgc
    13201 ttggaaatac gatatatctg aaattctgcc atgggttagc accgtcaagg tcagccgcct
    13261 cgtaaaggtc agggctgata gactggatag caccgatata catgatgata tcaaagccta
    13321 tgtatttcca tatggaaatg aacaccaagc agcagtttgc aagaaacggc ctgtcaacat
    13381 cgaaccacag tatcgggtca aggcccagtg tggtaaggat agtgttgagc gttccgtcag
    13441 gggtaagacc cttttgacct ttgaggaaaa atctttggaa tatgatagat accgcaacac
    13501 cgttcataag ataagggaag aaaagtattc ccttgaaaag tccctttgct cttatctttg
    13561 agcagaggat agacgccagc agcaaagcca gtgcctgctg aatgaatgaa ccaacaagat
    13621 aataaaggct gttcttgaaa gtttcaagat agtccttgtc ggtgaatatc gttacataat
    13681 ttgaaaagcc tgcatatgta gggttagtgc cgtatgggtc tctgttctga aaactgaata
    13741 tgaccatatc cagagcaggg ataaaggtaa acgttacaag caggattatc ggcagtgcaa
    13801 gaaacagtat agaagtatag aactttttac ccgcataagt ctttgaccac tgtgcaatgc
    13861 cttttctatt gccgttttgc atttgaggtc ctcctgaaca caattatcag gcacaggaac
    13921 aatgcctgta cctgataagg gatttatctt aagtcttagc cgagaacttt atctcttgca
    13981 gcagcccact tcttgtttac atcagcgatg atctcgttga aaccgtcgtc gcccttgcct
    14041 gcgaatgctg cctcagcaag cttgatcttg aagttgtcct cgtcgccctg ccatacgcca
    14101 acgcctgact ccttgtcgat ctcgtcgaac ttaccaacga ggtcatccgg agcaacagcc
    14161 ttttcaaaga atacagcgtc cttgaagcca gacagcatgc ttggaagctc cttgttctga
    14221 actgttgaga tcataccctc ctgctcagag aaacctgaat cagagatgaa ccactcaaca
    14281 tatgcctttg caagatcctg attcttggag tttgcactta cgccgagagt atagtcagga
    14341 ccttcctctg cgtatacctt tccgtcagcc gctgtgacag gaacaggcat ataaccgata
    14401 tcgtcagggt tctcagcctg ttccatgaac tgtgaaactg cccatgaacc cattaccatt
    14461 gtagcgatct tgccctcacc gaaccatacc tttgatgatt cccactctgt gttagcgtgg
    14521 tcttcttcaa gaaggtcagg ctttgagaac agatcataca tcagcttgta tacttcataa
    14581 tagccgttgc cctctacaaa gaggtcgctc ttatcctgaa gaagcttgtt ttcagcttca
    14641 gcaccgcctg ctgctgaaag aacaaggttc tgccactgag taatagtcca gcttgcgtcc
    14701 ttgaagtttg tgtagtaagg gatagcgtct gtgttatcct tgatgagctg aagatctgca
    14761 aggaactcgt caggagtctt aggagtttct gttataccag cctgctccca tatcttcttg
    14821 ttgtaaagaa cacctgtagc tgttccgcct actgcaagtc cgtaaacatt gccgtcagcc
    14881 gtcatcttct tgtctgacca gcgatatgtg tccttgaact cgtcataagt tccgagagat
    14941 acgaaatatg taggaagttc ctcaagctta aggcttgaag ggatacaaag aacatcgccg
    15001 taatcatctg tacccattct tgttgctaca tctgtatcgt agtccttgta tgcagtgtat
    15061 tcaactgtgc agttgtaagc ttcctcaaac ttgtctgtga gagcgtcgag cttgccgtcc
    15121 tcttttctgt cagttctgtt cgtaagaact tccagttttg caccctttgc gtcgaatgag
    15181 tctttgatat cagcctgcga tacttcaggt gtaacagcct tgctgcttga atctgtagag
    15241 cttccgcatc ctgtgaacgc actcattgtt cccattgata aaaccagtgc cattgcaccg
    15301 gcacatatct tctttgcttt catacttgat aacctccgaa ttagcttaat tttattaagt
    15361 aaattaattt gtcctcaacg acaata
//
LOCUS       DAWEMR010000048        15171 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1626_length_15171_cov_10.631649,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000048 DAWEMR010000000
VERSION     DAWEMR010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15171)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15171)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15171
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1626_length_15171_cov_10.631649"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1282..1370
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2836..2845
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7361..7370
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7929..7938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4851 a         3270 c         3156 g         3775 t
ORIGIN      
        1 tccatatcga ggaaatccac gggctttgtt gcgctttttt ttacagcgcc tttttgttgt
       61 cagtgtacat aaatgagtac aaactgatat ggcacgattt gtgtatatgc acaaaggtga
      121 taaatttgtc acttttctat tgactttgca aggcaaaagt gatattattg tcacataagg
      181 tgataaataa atcaccttgt atatgcagca gtttaaatca caaactgcga taaagaaagg
      241 gcacacactt accaatgtga taaaccttat cgtatctttg ggaacgcttg cgctgatcgc
      301 catatgcata acaaagatga tatccacggt cggcaaaaga gatacaaatg acgagcttac
      361 tcagcagaca acagcaaagg tcaatcagac ttttataacg ttcctgttca cgattgcctg
      421 cctgtttatt tcagttatga ctttgttttc cgatatttta ggaagattta aggtcacact
      481 aaacgaatat gttataacgg gtttgatatg gttttgctat gcggtgtaca attttatagg
      541 gctgtatttt gaaagtaaat tcgatgatga cgagaactag aggataaaaa tgaaagcgaa
      601 aaaagaaaag aaaaagataa ctctgagaga atatcacaaa aagcgtatgg taacaggaat
      661 tgcaatactt gtcacagaga tatttgaaaa cacggtggag cattacaagc ttgataatac
      721 aacttgggcg acggtgatat tcgttgtgtt ggctgctatg acggctttat ttgcagtaat
      781 atggctgtat gcaagctgtc agaaagttga caaagaggac gaaatggcaa aagaaaatat
      841 gctcaaggca catcaaagta tcacaaaaat gtttttgata atactagcag tagcgatctt
      901 tatatcaatg ttctgggaag gaagtataac cataaagata aacagcgaca gactatatca
      961 agtatggttt atattctggt tcagttatat gacccttgaa agcggatttt tcctccacca
     1021 agagggcaag ctcagcaccg atgaggagga cgagtaatgc cggagctaaa aacaaaactc
     1081 aaggaatatc gtgccaagct tgacatgaag cagggtgacc ttgccgaaaa agtgggcgtc
     1141 aggcgtgaaa ctatcatacg tcttgaaaaa ggacaataca atccgtccct aaagcttgca
     1201 atggatattg caaaggtatt tggtgtatct gtagaggata ttttttcatt tgaagatgaa
     1261 taaagctgaa aatacagtaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggtttgcag
     1381 gattttggtg cagaagaaaa tcatatactg aaaatgcaaa tacagacata taagcacata
     1441 ttctaacgta gatagataaa tattgaatat acgttgctta tgctatgatt atctttagtt
     1501 tacgccatat tgctgtgtat attagtgtaa aaggcaaaag tgaaatgaat tgtgagaaat
     1561 cgagagaaat ggagcaaaac gaagagaatt aaagagaatg aggaatataa attaaaaatt
     1621 cgacgttttg actattgaca atcgtgaaaa gctgttgtat aatagacaac gttgtgagtt
     1681 cacagggcga aaaatgcggc tgtgaacaga ctttttaaaa ttctgaatgg aggaaataat
     1741 atgtcaactt atatgccaaa agcaggcgac attactcgca catggtatat tctcgacgct
     1801 gaaggtcagt cactcggtag agttgctgct aaggctgctc acattcttcg tggcaagcac
     1861 aagccaactt acgcaccaca cgctgactgc ggcgaccatg taatcatcat caacacagat
     1921 aaggctgttc tcacaggcaa gaagcttgat cagaagtctt acaagtggca cacaggctgg
     1981 atcggcggtc ttaaggagat caagtattca gatatgatgg ctaatcagtc agacagagct
     2041 atgacaattg ctgttgaggg tatgctccct aacaacacac ttggcagagc tgctgcaaag
     2101 agactcagag tatacaaggg tgctgagcat aataatgctg ctcagaagcc tgttaaatac
     2161 gaactgtaag gaaggagcaa tttagatgta cgaatcaaag cagccatatt tctatggcac
     2221 aggtagaaga aaacattctg ttgcccgtgt tcgtgtttac gagggtacag gtaagatcac
     2281 aatcaacggc agagacattg acgattactt cggtcttgag acactcaagc tcatcgttcg
     2341 tcagccattt gctgtaacag atacaatgga taagtacgat atcgtttgca cagtaacagg
     2401 cggtggagta acaggtcagg ctggtgcaat cagacacggt ctttcaagag ctcttcttca
     2461 ggctagcgat gagttcagac ctctcctcaa gaaggaaggc ttcctcactc gtgacccaag
     2521 aatgaaggaa agaaagaagt acggtctcaa agccgcaaga cgtgctccac agttctcaaa
     2581 gagataattt cattctcatt tatcaaaccg tttcgcagaa atgcgaagcg gtttttttat
     2641 accctatcct aactgtcaaa atgcacaacc aaaacatctg ttcgttgtca atttagctga
     2701 atatccccct gcttattgta aataacaaca aaatatgata taattattac tgataaatta
     2761 acttttagga gataactgct atgacgaatg aatacataaa accgcccgtt gagatacgct
     2821 acaaaaatga acttgnnnnn nnnnngggtg ctgtcgccaa aggcagtgcg aacatttata
     2881 ttaggcggaa tcatcaaaac tgccgatggc aaaatgcaga taaagcgcaa attctatggc
     2941 aatgacgcac ttgttgagcg ctgtataatc accctcgccg gaaaccgtgg acttatgctg
     3001 gtgggtgagc caggcactgc taaaactatg ctctctgagc ttctctctgc tgcctgctgc
     3061 ggcataagca cgaacacagt tcagggtacg gctggaacta ccgaggatat gataaaatat
     3121 agctggaact atgccatgct tctttcaaaa ggtccgtgcc gtgaagccct tgtgcctgct
     3181 cccctgctca taggcatgga acaaggcatt ttcgcacgat ttgaggaaat aactcgtacc
     3241 cctgcagaaa tccaggatag ccttatttct gtaatgagcg accagatact caatatccct
     3301 gagcttgaaa acgaaggact tgttttcgcc aagcaaggat ttaatataat cggtactgca
     3361 aacacccgtg ataagggcgt aaatgaaatg tcaggtgctt tgaaaaggcg tttcaatttt
     3421 gaaactgtcg agcctgttcg tgacgtaagg ctcgaaaagg agatcattat ccgtgaagca
     3481 caagaccttg caaaaacagg tcatatcgac cagcctatag acaccgacgc tgctgaactt
     3541 cttgctgtca catatcatga gcttcgtgag ggcgttacct cagagggaac aaagcttgaa
     3601 aaaccaaacg ccgttatgag tacagccgaa gctgtgtccg ttttctatca aactatgagc
     3661 agtgcatatt attatggtga cggaaaaact gatctcaaag agcttacgca gaatctttta
     3721 ggagcagttt gtaaggaaaa caaagacgac cttgaaaagc ttaaaagcta tttcaggatc
     3781 gctgttaagg aaaaaagtgc aagtgaggga ggcatatgga cagaatatct ggatacagcc
     3841 aagtatctga aataatacct ttcgactatt ctcagccatt tatgcttttt cctgtaagac
     3901 accacagccc tgtctgctcg tggcagctca tcagagcaat taaagaatat cagcccgatg
     3961 tcatacttat agagggtcct gaaaacgcca atgatatgat aggtgtgttg acggacgaga
     4021 gaacaaaact ccctgcggct ttctattatt actacaagga cagaaaaaag tttatcagtg
     4081 atgaagccga agactataaa tgctactacc cttttatcta tgcttcacct gagtataacg
     4141 ctctgaaaac agccgcagcc atggatatag aagcacgctt tatagatctt ccctacagcg
     4201 agatacttat taccaccgca gaaaataaag gccttcgcag taataaggat aaacacagct
     4261 acactgatga cagcagactt atatacagca aattctgcaa aaagctttgc gagaaaacag
     4321 atctaaggac ctttgaagag ttctgggaga agtattttga gatagagggg ctcagactat
     4381 ctgtacaaga ttttgtacag caaatgtaca cctactgcat tatcacacga aatgacgaaa
     4441 cagaagatga tcttgcggca gacggcactc ttgcaagaga aaaccatatg gctttgcgta
     4501 tcaaagaggc acttaaagat aacaaaaaag tccttgctgt aacaggcgga ttccacagtt
     4561 ttggacttta tgagcttttg aaaaatgata atatccaaaa agaaaagctt cacaagctct
     4621 ctcaaaaaga tgagggctgt ttccccgtag cttattctta tgaagcagca gacgctctca
     4681 gtggatatac cgctggtatc cagcgtcctt atttttatga ttgcgtgatg aacaagctaa
     4741 tccactgcga tgacccggca ggtgtttact gcgacacagt tcttgacctg ctcataggta
     4801 cagtgagagc ctgtgataaa catgatatcc cagtgtcaat ggctgacgct tctgcagcgc
     4861 agtctatgat gagtggactt gccgcacttc gtggctgtca tgaatgtgga ctttacgagc
     4921 ttgaggacgc cataacctcg tcatttatca agggagagaa aacaatatcc tccgccctgc
     4981 ctatcgacct tatgcacaaa cttgccacag gcgacaagac aggtcatata ggtgacataa
     5041 accacgttcc tccgcttatc gctgattttg aaaaacagtg taaaaggttt cgacttaaaa
     5101 taaaaacagt tacccctaat aagacggaag tttcactttt taccactgca aacggaatgg
     5161 aactaagcag atttttccac agaatggtgt ttctaggcac agattttgcc cagcgaacaa
     5221 aaggacctga tttgcatagg cgaaaagaca gaagccgtgt ccgtgaagaa tgggtataca
     5281 aaaaagtccc tgccacagac gttgcactca tagaccatac tgccgacggc ttcaccattg
     5341 aagaagcctg ccgcacctgt gcttcaagaa cacttcgcca tgaaaagcac tgtgatgttg
     5401 cggcgcatat ccttgtggac tgcttccaaa tgggcttaga actctctgat gatgacaagg
     5461 cttgtgccga gaacattctt aactcagacg gtgacttttt ctctgtaggc agaggtctaa
     5521 ggtattttat cactcttatg gaactccaac agctttataa taccgagttt tccgccgcag
     5581 aaaattgtgc aaagcggtgc atgacaagaa taatcacagc actgcctgat atggcgtcgg
     5641 tgaaagacga ccacattgca gaatgtgcag cgataatgta cgcaatgcaa aaagctgtca
     5701 ctgacggctt tagagaatat cgtcaggact atgaaaacgc cctgctttcc ctctgcggca
     5761 aatctgataa agacccattt gtttacggca cagcacttgg catactttat gcctttgacc
     5821 cccaccgccg caagcttgct gaacagacaa tgtcaagcta tctcaaaggc gacaggaatg
     5881 ttcagataca aggagcagag tttcttaacg gacttttcaa tgccgcccgt gatatcatca
     5941 tgacggacga ttctttcatc agaatgacag acacgcttat ctgcggactt gactatgacg
     6001 attttataga aatacttccc tccatgaagc ttgctttcag ttgtttcact ccatatgaaa
     6061 tacagcaaac agccaccgct gtcgcaaagc tttatgacgc tgacagtgct gagcttcttg
     6121 tggaaaaacc tataaacgaa agactatata gcttcggtgg gaaaatagat aaagagatag
     6181 tcaaactttt gtctgaggag gaaaagccat gacagatgaa aataaagcca tatccgtcaa
     6241 ccgctggcgg ctcgtgctgg gcggaatgtc tgacaaaagc ctttccttta ccggtgacaa
     6301 agcagagata ataacgtttc aggatatgga aaagctactc gattatctct atagccgtgc
     6361 gcaaggtgac gatgtaagga acgagaacag caggcaaggc ggtcagggtg gcagtgtgct
     6421 aaacgccgca gaatggataa caaaagtcag aacgcttttt ccaaagcaga ccgccgatgt
     6481 gcttgaacgt caagctttgg aaaggttcaa catgaccgag cttcttaccg ataaaaaggt
     6541 tcttgaagaa atgaagcctg atatagcgct gcttaaaact atcattcagc tcaaacatct
     6601 catgaacggc gaggcattgc aggcggctgt aaaaatagca aaacaggtgg cagacgatat
     6661 caaacaaaag cttgatatga ccataaagcg aagccttaca ggcaggcttg ataaaaacac
     6721 atcgtcagtt atgaaatgct atgcaaacct cgatttcaaa aagactatcc gccgaaacct
     6781 gaaaaactat gacaaaacaa gcaaccagct tatactgaaa gatatctatt tcagtggacg
     6841 tgtgaaaaag cataacaaaa agcgcattat catagccata gacgaaagcg gttcaatgct
     6901 cggttcagtc atctatagtg cagtaatggc gcagataatt tccgaactgc cctttgcaga
     6961 ggtgaagctc atcatatttg atacaagcat cgttgacctc tcagaccatg cagacgaccc
     7021 tgctcagaca ataatgagcg ttcagcttgg gggaggaacg gatattgcaa aagccctttc
     7081 ctactgcgaa agccttatca ttacgccccg tgacacttgc gttatcgtgg taacagacct
     7141 ctacgagggt ggcagtgaag caaagcttat gaacgtcagc aaaaatataa tcacaagcgg
     7201 cgcacatctc tcattcctta ccgcccttga tgaaaatgcc gaccctgcct atgacaaggc
     7261 gacaggtcag aagcttgcag atatgggctc attcgttggc ggtcttaccc ctgataagct
     7321 tggggattac atcggaaaaa tatttgcata ggagatgaca nnnnnnnnnn tgacatcttg
     7381 gacgtaatct ataaacagat tggcattgca aacgaacaaa cgcttattga actttcaaac
     7441 aagggcattt acaaacgtgc gttgaaggaa tccaaaaaca tgaccctttc ggctgaaaaa
     7501 atccatgaat gttacatagt aaaattagac ggtgaaacct gcaccataaa atcacctctt
     7561 gatgaaagtc aatgcagttg tatttcaaga ggaatgtgca ggcacattat aacagccata
     7621 ctgcttttga aagcacaact tccacagtac gacggcgagg atatcactcc agaggtcata
     7681 acctcagagc ctgaacaagc agaaattgaa ctccccgacc aacctgaaat aaatccgctt
     7741 tctcaggata cccagaaaaa gataaaagga tgtgccgaac agtgcttaaa aataatgtct
     7801 ggaatattta ccagaggtct tgtaagagcc gaagagagcg accccgataa ttttgagctt
     7861 gccgcagttt cctgccacgc tttgagaatg gctgaggcag aaagacaaat gcgtgaactt
     7921 ggcagccgnn nnnnnnnnac tgtgtttcaa ggcgagcttc attcagtccg caggtattca
     7981 ctcacaaaat gtttgaatat gctgacagca tgaacaagct tataaaagcc gacatcacag
     8041 aagaaatgct cggcaccttc cgcagagaat acaccgacta tgaggggacg ctccaactcc
     8101 tgcctatagg cacaagaaat gtcagtggcg aatacactgg ctgtatctac tattttctaa
     8161 ataaagacag aacttcgccc cagcgctttt tcacctacag cgatctgcgc cccactttct
     8221 atgaaagaaa gtcaagacga tttgccgaaa gcacaactgt atgggatctt gacagctccc
     8281 taaacaacta tatgcacacg gaactaaaac taaaaaacgc caaggtgagc atggggcatt
     8341 tgtcctcatc gtcaaagacc aacgccatag gcgcaggccc tgcacagcta aactgttttg
     8401 ccctccgtga gcttattgtc agcgatttca aagagcttgc ggaaaagatc tccgcaaaca
     8461 aaagcgacga ggagaccgac cggctttatt tcgttcaccc caaagagtgc gtcgcttcat
     8521 attttgacaa gcacactcaa cagcagattt ttattataaa agacggctgt gatagacaaa
     8581 tatctgtcac cgcaaaatac acagcagaaa atcgtgaatt tatttccacg cttgaaacca
     8641 taggtggcaa aatgctaaaa gaaaagcaca aaaactacgt cctgctggct caggggtata
     8701 tcgaccacgg caggctaacg cttttcccaa tagaggtcta tgattttatt gatccgcctg
     8761 acaatgttcc tgtacccgtc gagaatgata cagaccaaga ctatggaatg tgtagtgagc
     8821 ttcttgacac caccgaagaa accgacaaga ggatagtcac ggcaatggag tgcggagtga
     8881 actctgttat tacagacgaa cacgctcaaa gcatacgcca atgcggactt gaagaacttg
     8941 caaaacgtta tgaatgcttc acaaagctgt gcgaaaatgc aagacacacc accgcagaca
     9001 aaagccttga tatcttcact gccgcaggca acacaatgcg gtatatcaga ctttgcacac
     9061 aaaaattagc actgttcagt gcgataaata atatggagga gaaaaaatga tctacaacga
     9121 aagcaccaaa agctttataa agcttgaaac cgccctaaag gagcttggtt ttgaccagca
     9181 gaccatagac cttgccattg aatatcttga catcacaaag ccaagaaaca atgacctgat
     9241 gagaaaaatt acaacaagat actatccaaa actcatgcag tatagttatc gtgaaaagct
     9301 tatcaaagct gtaaaaagtg agatatcaaa ttcagatagt gaggagctta aagaccgtta
     9361 tattattttc agttcaatgg caacgggtcc atatttccga tatatgacag atgcgctgtc
     9421 tggcaatgcc tatatatatt ccgaaaacaa agccatgtat atgaagccgc tcactgattt
     9481 ttatggtgag aacgcagaag cagtctggat atcaaccgag atctcaaacc gctcaaatga
     9541 ttctatcagc gttttcactg taaatcatcc tatctattcc gcagacgatg ccataagaac
     9601 attcaacttc gttagccctg acgatatcat agcacagctt ctcctgtgcg tttacgcatt
     9661 ggacgcaacc gagctgccta aaaagagcct gctcggcaaa acaaaaattc ctgatacagc
     9721 cgaaattgcc ataaaaaagc tttgcgaagt agtcgaaaaa aagcccaaaa caactacagc
     9781 cgttttctgt ctttttcttg ccgcatatgc agaagcgtca tattttgtaa aagaatatcg
     9841 tgcatttttc gataaaaaca ttgtgaaata ttacgacgat cttacccaag ggctgatata
     9901 tatgaagctt tccaccacca ggatattcga ccttatcgaa gctcaaccac agtgtcttac
     9961 agatagatac attctgcact ttgtcaaatc acctgacaaa aatgggaatt atccagcgca
    10021 aatggtaaag ctcgcaaaag atcacccaca gcttttcata gaaacaatga aaaacgctcc
    10081 atttgacaca gcgatcacaa tggagaaaat gctcaaagcc accaacccta aatataacgc
    10141 agaagcaatt ggtcttaaag ctacccatca ggaacgtatc gccaaactta ttgccgaaca
    10201 tttcaagcac agtgacgaga tatacgcata tatcatgggc aaagcagaaa tagacgcgat
    10261 actccccttt gcagacgaag aagcctgcga gggcagacat aagcaaatgt actatattgt
    10321 tgcatatgga gaggacgatt ttatcatccg ctgtatcgcc gccctttcca taggagcgtc
    10381 taaagataca agtgaccccc tccaacttat aggcaacgat ctttattata acaccgagtt
    10441 catcgtacgt gcaatggtaa gatcaggtct accgatagat ataattataa gatcccttgg
    10501 ctggcagata gaaagctctt actgtaaaga agccatcatc aaaaagataa ccgctgtcat
    10561 gccagaattt aaagacaaag ccaccattat cgacgtaagc aaaatggctg tgtccagccg
    10621 acttttgtat ataaggcttc ttgatatcgc agacagcaac aagtacaaag acgaattttt
    10681 tgccctttgt gacgataaca gcaaggtaat aaaagcagag cttgtgaaag ttatatccgc
    10741 ccacaaggat tggcacgatg atgtttgcaa acttcttgct cagaaaaaat caaccaagcg
    10801 tgaaactgca cttatgataa ttgaaaatca aggtgctgac gcctaccgca cagagcttga
    10861 aaaagcctat gccacagaaa aatccgacaa actaaaatcg aaaatatctg aaattttggg
    10921 cagcgaagca aaacctgccg aaatatcaga tgaagacctt gtgactgcgc tcaccaaagg
    10981 cacaaaaagc aagaaagttc tctggctttt tgaacagcct tttgcaccta ttcacttcac
    11041 caatgataca gtcgcagagg atatctattt gcaggctctc ctgcttacat atgcaaacgc
    11101 caacgaaggt actctgccac ccgagggcaa ggcacttgcc aagaagctca aagctgatga
    11161 gcttgaaaca tttgcccttg aagtcctgag ccgctggctt gaaaaaggtg cagaagcgaa
    11221 aacaaagtgg gcaatgtatt ttgcggccat ttacggcggt gatgaagcaa taaattgcct
    11281 gacggattac ataaaagaat ggtcgaaaca gtcattaaat atgcgtgttg cccttgcggt
    11341 taaagccgtc aatgccgtag ctctgaacgg cagttcctat gcacttatga ctgtagacaa
    11401 tatttcaaga aaatataaga gtcgtgcggt cagagctgcc gcagttgacg cacttgccaa
    11461 tgccgctaaa cagcttggct tgacaacgca ggagcttgcg gacaagatag tacctgacat
    11521 gggctttgac gagaaaatgt gccgcacctt tgattttgga agcagaaaat tctccgttta
    11581 tctcactcca cagcttgata tagaaatatt cgagggagag aaaaagctta aaaatctccc
    11641 taaaataggc gttaatgacg accctgccct cgcagaaaag gctactgctg atttcaagga
    11701 aatgaaaaag caaatgaaaa ctgttgttga agcacagaaa cagcgtcttg aatatgtgct
    11761 tatgcttgac cgtaagtgga cagcagaagc ttggaaagct ctctttgtaa aaaatcctct
    11821 catgcactgc tttgcgatag gtcttatatg gggtatttac gaaaatggct gtcttaaaac
    11881 ttcgttccgt tatcttgacg acggcagctt tacaaactca gatgatgatg agatagagct
    11941 gtccgaggtt atgcagatag ggcttgttca cccgctggag cttactgaac atgaaaaaga
    12001 agcttggctg gaacaacttg atgattacga gataatacag ccattcgacc agctaaaaag
    12061 aaaggtatac aaagtagcgg aaagcgataa ggataagacc gcctgcgaga tcttcaagaa
    12121 taccgagata acgaacacca ctctagtcaa ccgcatgacc aaagcaggct ggtataaagg
    12181 tcaggcacag gacgcaggct ttttctatga gttcataaga aatgatatct cgggcaagaa
    12241 gaaagatccc gacggcaagc tcgtcaacat aggcatgacc gccgagctta aattctcagg
    12301 aacatacatc ggctattacg agatagagga cgtgaccgtg gaagagcttt actttcgact
    12361 tcccgatgct gcatacaatg acaacatgaa acttggcgat gtcaatccaa gatactacag
    12421 cgaagttgtc ttacaactta aaaaagctat aaacaagtag gcataacaaa aggcagttct
    12481 gatttttttc agagctgcct tttacttatt tattttccac aggaagtccg tgatctcctg
    12541 caatgtgtct gcctgccctg ttcacacaat atatgcaaac aggaactatg tacaagcaag
    12601 ccgctatcca tgccgcctga tctgtgaagc agattgcaag ataaccgaat ttcggcacaa
    12661 atatcatact tacagaaatt cgagctatca tttccgtcac tcctgagaat atagcaagtc
    12721 cagaatatcc aagaccctgc gtacacattc tgcaagtgtt gagtataccg agcgtccaat
    12781 agaaaagacc tgcgtagaaa agatatttgg ctgaagcagt aagcactgct gcgtctgact
    12841 tattcacaaa gataagcgaa agctctctgc cgaagaatat aagtacaata ccggctatta
    12901 ctccatatgt catgccaacg ccaactccaa tgcgtatacc ctttttgata cgtttgagat
    12961 ttcctgcacc gagattttgg cttgcaaaaa cagatgcacc tgttgcaaaa gcgtcataag
    13021 gacacattgt gaactgcttt atccttgccc cagcagtgaa tcctgacaca taaatgcttc
    13081 caagcttgtt gttagccgac tgcatgacca ttgagccgat agccgttata gaatattgaa
    13141 gtcccatagg aaagcccatt attataaggt tcagcaccgc aggctttgac atcgctctgt
    13201 cctcttttga aagcttaagc acgtttacct ttttcataat aaatacagca catagtattc
    13261 cgcttatcgc ctgagcaaca acagtagcga tagccgcacc catgcagccc cacctgaaca
    13321 cgataacaca cacaaggtca agtgcaatgt tcaaaaccgt tgatatagca agaaatacaa
    13381 atggcgtttt gctgtcgcca acagaacgca atacgcttga aagcaggtta tacaaaagtg
    13441 tgaacggtat gccaagaaat atcaccagaa gatatttata tgcatcatca aaaatattcg
    13501 acggagttga aagaagcttc aatatctcac tacaaagcac cgcacaaact gtggtcaaca
    13561 caaacgcagt aacggcggtt ataacaatag aattgtaaaa atactgtttc agctttttta
    13621 tgtcccctgc tccgaaatgc cttgcaatag gcacgccaaa gccgcaggct gttcccatgc
    13681 agaaaccaag caccaaaaac tgcacactgc ttgaagctcc gacacttgca agagcgtcag
    13741 aaccaagaaa tcttcccacg atggcagcgt caataagatt gtatgtctgc tgcaacaaat
    13801 ttcctaaaag cagaggaagc gcaaagctca ttataagctt gaacggactg cctgttgtca
    13861 tgttttttgt cattactttt cctccaaaaa tctaactatt acatttatca tctttaattt
    13921 ttactatcac attctatcat caatatatga aaaaatctat aacacaaatt aaatttactt
    13981 ttgtgatttt aaacaaatat aataacaaaa aacatacata ttgcacataa aaaaggcaag
    14041 catatttcag cctgcctttg tgtagcttac gctttatacg ttaaatctga aaagaacgat
    14101 atcgccgtcc tgcataacgt aatccttacc ctcaagacga acaagaccct tttccttagc
    14161 cgccgtcata gagccacagc tcataagatc gtcaaatgaa acaacttcag ctctgatgaa
    14221 gcccttttca aagtcagtgt gtatctttcc tgccgcctgt ggagctttag ttcccttttt
    14281 gatagtccaa gctcttacct caggttcgcc tgctgtcagg tatgaaataa gaccaagaag
    14341 tgaatagccc tccttgatta ttctgttaag acctgaagtt tcaagaccga ggtctgcaag
    14401 gaacatttcc ttgtcctcat cgtccatgtc agatatctca gcctctatct gcgcgcatat
    14461 cgggaacaca gccgagcctt cagcttttgc gatctcgcaa acttccttgt aatgctcgtt
    14521 gctttcgata ttatttctga aatcatcttc gcaaaggtta gccgcataga taacaggctt
    14581 aagtgaaaga agtggtgttt ctttaagtat ctccagctcg tcctctgtca tgtcataaga
    14641 acgtgcaggc ttaccgcttt caagatgctc tttgagcttt tcaaggaact ctacagcaga
    14701 agcaagagat ttgtcgcctt tcattgcctt tttagttctg tcaagctttc tctcgataag
    14761 ctcaacgtct gagaaaataa gttccaaatc tatagtttca atgtctctgc gaggattgat
    14821 agagccgtca acatgaatga tgttagggtc gtcaaaacat cttacaacgt gaacgatagc
    14881 gtccacctct cttatgtttg aaagaaattt gtttccaagt ccctcgccct ttgaagcacc
    14941 ctttacaaga cctgcaatgt caacaaattc aagtgttgca ggagtaaact ttacagggtg
    15001 atacatctca gccagcttgt caagtctttc atcaggcacc gaaacaatgc ccacattcgg
    15061 ttcgatcgtg caaaaaggat agttcgcaga ttctgcaccc gcatttgtga gtgcattaaa
    15121 aagtgttgat tttcctacgt taggaagtcc taccatgcca agtttcatat c
//
LOCUS       DAWEMR010000049        15063 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1640_length_15063_cov_9.346815, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000049 DAWEMR010000000
VERSION     DAWEMR010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15063)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15063)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15063
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1640_length_15063_cov_9.346815"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11513..11522
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12689..12698
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4171 a         3291 c         3060 g         4521 t
ORIGIN      
        1 aaaacagcaa aaataacaag ccaaatttgt acaatgacac aaaacatagt aaaacgtgtt
       61 gacaaaccac tataaatctg atatagcata aatgtcagtt atggcgatgc catagcttgt
      121 gagttgaaat atatttgtat ttggtacgac ctaataattg gttgtaaata agaagataag
      181 ggtgtgttcg ttgtggcata cccttatttt tttgctaaaa atagaaaaaa tgaaacaaaa
      241 agcagggcat taaaataata ccctgctttt tgtttcattt tttacaaatt gtaaagtgta
      301 ttgtttaaat attctctgcc acatctcata ggagggagaa cactttccat tacgccagtt
      361 ttttattgtt gtaatactaa cattgcatat cttagccaat ttgcgtatag atagattgtg
      421 caattccata taggagcaaa ctttttcctt tgagtgtgtt ttaaaggaca aataatcatt
      481 cataagattt atgtttagta catcagatat ttttttcaaa gtatcaaagt ctttatattg
      541 atcctttact tcgtcacgtt catacttgat gtaattatca taactcaatc ctgttagttt
      601 acatatttca gttatagata aaccattatt tatccttgct tttgcgatgc gggtaacaag
      661 cctgtgatta tcgtttccac gattcttcaa ctgcaactgc gcaagcaact gtagcaccaa
      721 ccattgggtt gttacccata ccgataagac ccatcatctc aacgtgtgca ggaactgaag
      781 aagaaccagc aaactgagcg tcgctgtgca ttcttcccat tgtatctgtc ataccgtaag
      841 aagcaggacc agcagccatg ttatctgggt gcagagttct acctgttcct ccgcctgaag
      901 ctactgagaa gtacttcttg cccttctcaa gtctttcctt cttgtatgta cctgcaactg
      961 ggtgctggaa tcttgttggg ttagttgagt tacctgttat agaaacgtct acgttttcct
     1021 tccacatgat agcaacgcct tctgtaacgt cgttagcacc gtagcagttt accttagctc
     1081 tcagaccgtc agagtatgcc ttgcggaata cctccttaac ttcgcctgtg tagtaatcca
     1141 tttctgtttc aacatatgtg aagccgttga ttctagcgat gatctgtgca gcgtccttgc
     1201 caagaccgtt aagaataact ctcagaggct ttgttctaac cttgttagcc ttctcagcga
     1261 taccgatagc accctcagcg gcagcgaatg attcgtggcc tgcgaggaat gcgaagcact
     1321 ctgtgtcctc ttcaagaagc atcttgccga ggttaccgtg accaagacca acctttctct
     1381 ggtcagctac agaaccaggg atacagaaag cctgaagtcc ctcaccgata gcagcagcag
     1441 cgtcagcagc tcttgtgcag cccttcttga tagcgatagc gcagcctact gtgtaagccc
     1501 acttagcgtt ctcaaagcag ataggctgaa tgccctcaac gagcttataa atgtcaagac
     1561 ccttagcctt gcatacgtca gcacactctt cgatagtgtt gataccgtac tgattgataa
     1621 cagcaaggat ctgcttttct cttctctcat atgattcaaa taaagccatt gtaacaaatc
     1681 ctccttattc ttttcttggg tcaacgatct taacagcgtc agctactctg ccgtactgac
     1741 cctgtgcttt ctcaaaagca gtgttagcat cgtcgccagc cttgatgaag tccatcatct
     1801 tgccgaagtt tacaaacttg tagccgatga tctcgtcatc ttcgttaagt gcaagacctg
     1861 taacatagcc gtctgtcatt tcaaggtatc taggaccctt tgcaagtgta ccatacatag
     1921 tacctacctg tgaacgaagt cccttaccaa ggtcctcaag accagcgcct actacaagac
     1981 cgccctcaga gaatgcactc tgtgaacgac cgtaaacgat ctggaggaaa agctctctca
     2041 tagctgtgtt gatagcgtcg caaacgaggt ctgtgttaag agcctcgaga actgttctgc
     2101 ctgggaggat ctccgatgcc atagcagcag agtgtgtcat acctgagcag ccgatagttt
     2161 caacgagaca ttcctgaata acgccctctt ttacgttaag tgtcagcttg cagcagccct
     2221 gctgtggagc acaccagccg ataccgtgtg taaagcctga gatatccttg atttccttag
     2281 cttttaccca ctttgcttct tctggaattg gagctgcacc gtgagcaacg ccctgagcga
     2341 tagggcacat tgtttcaact tcgtgagaat aaaccattat ttacataact cctctcaaat
     2401 gattttggtg taaatgttgt atattacata cactcatatt ataacgcata tttcgccatt
     2461 ttgcaatagt attttatgat ttttaatatt tttttctcat tctgtggtaa ttataacgct
     2521 gctgagaggt gcgagtctga gtggcagctt atagtttgtg gtataaagac cgctgtttat
     2581 gcagtttgat ccactataaa tatcacggtc agagttgaga agctctttgt aggctccgtt
     2641 ttgctcgacc ttcaggctat agttctgata gcttccggaa aaattgaaca ccatataaag
     2701 cgtcttgccg gaagggtcac ttctgcggta ggcataaatg cactgatttt tgttgtctgc
     2761 gtctatccag cggaaagatg tggaattata gtcggactta tacagtgcag gctgttcaag
     2821 ataaagcttg tggagtgctt tcacatactc gtgtaatgaa tcgtgtatag gatatgtgag
     2881 caaattccag ccaagctctt tttcatcacg ccattcaaga tattcgccaa gctcgttgcc
     2941 catgaagttg agcttcttgc cagggtgagt gaactggaaa gtatacagtg ctcttatggt
     3001 agcaaactgc tcctgctgac tgccgaacac cttatccacc atggttttct tgccgtgaac
     3061 tacctcgtcg tgggagtaag gaagaatgaa aaggtcatta tagaaataac tcatggagaa
     3121 ggtcatttta ttgtgatgat ttctgcgttc aaaaggcgga gtctttatat aattaatggt
     3181 atcgttcatg aagcccagat cccatttata gtcaaaacca agaccgccga actcaacagg
     3241 ggcagttacc tttgtgtagt ttgtggaatc ttctgcgaaa agcataacgt cagggtgcat
     3301 tttctgaatg gcgtaattag tgttcttcaa gaaccatata ccgctgtcgt tcacagcgtc
     3361 atcagtgtga ccgtgacgat agataaggtt tgcaactgcg tcatatctca atccgtcaac
     3421 atgatatttt tctatccaga aattaactga ggattttaca aagctaagca catgaggctt
     3481 tgtgtagtca aacagggcag taccccactc ggagtatctg tcatactcat tgtcgctttc
     3541 aaagaggaag cttccgtcat atatgtgcag ggcatagaaa tcacggacaa aatgtacggg
     3601 tacaaagtcc agaataatgc cgatattttc cgcatgacaa gcgtctatga acttcataag
     3661 ccctctaggg tcgccgtaac ggctggttgc ggagaaatat cccgacacct gatagcccca
     3721 tgagccgtca taaggatatt ccgtcagtgg cagaagctcg atatgggtat agcccatttc
     3781 ctttacataa ggtatgagca ggtctatcat ttcttcatag gtgtaaaagc ggtcggtttc
     3841 ttcttttccc tcttttatgc gccacgagcc gaaatgcacc tcatatatgt tcataggctt
     3901 ttcatagcat ttgtcacgct ttgagagcca ttcatcatca ttccagctat agctgtcaat
     3961 atcatacacc acagacgcat ttttcggtct tacctcgctg tacaccgcaa aagggtctga
     4021 gcggtagtgc ttttcaagag taggcgttgt gataacatac tgataaatgt caccctcttt
     4081 tgcagggcgt tcaacgctcc ataccccacg ttcatcacat tccataggga taatatctcc
     4141 attcaggcaa agctctacct tccttgcatg gggcgcatag gtggaaaatc ttatgccgtt
     4201 ctctattttt actgccccca gggcttcata cgctctacag ctttgaccat ttataaattc
     4261 tgtgatgata ttgtttttca tgccgtttcc ccctgtgaaa aatatgataa tgataaaata
     4321 tttataaaaa gttattgaaa tttcataaat ttatgatata ataaaagttg gcgcgaattg
     4381 tctgcttttt tgacattaat ccagcttagt ctgatttttc ctcaaaaaag accttttgtc
     4441 gttatgaatc tgaaaggaat gtagttaaat gtcaagctct gcgataacca aaagtgcttt
     4501 gtgtgattca ctgaaaaaac tctgcgagca aaaacccttt gataaaatat ccatatccga
     4561 tataacaggt gagtgcggtc ttaaccgcca gtctttttat taccattttc aggataaata
     4621 tgagcttttg agctatatat atcttcacga gcttttcgct gacatcacca aaggcgtaaa
     4681 ctacgacaac tggtatgagc gtcttgaagg atttctcgta aatatgctca aagacaagcg
     4741 cttttactca aacacgctaa aaagcaacga aaagatattc gagcattatc ttttcagaag
     4801 tatgcacaag ctgttcatca gatttttcta tcacgctgtt gctataacca gaaagcgttc
     4861 cgataaggcg aaattctttg ccgattttta ttctcatgga tattgcggaa taataataga
     4921 ttgggcaacg ggcggaatga gagaaactcc acatgacgta atgttgaaaa tgcgtgagct
     4981 tgcatacgaa aatgttacca ttggtgaggg attcagacag aacctttgac aaaagtcaca
     5041 caatcactct gttattttat catattttat gtaataagtg aatagacaaa cccaaacata
     5101 cgacaaaaaa agcgacagat ccagatttct gtcgctttta tttttttcta ctttgaagct
     5161 tttgattttc ttctgtcaat aataacgcat gctatgaata tgagcaatcc tgcccctgag
     5221 agaactatgc ccttgtggag caacggtgcg gtaaaggtta tctttatgct gtggctgccc
     5281 tttggtatct tgaagccaag gaaagcagtg tcggtctttt cacactgcac ctctttgccg
     5341 tcgatctcag cggtgaagcc gtcggtatac ggaatggaaa tggtgaaata gccgtcattt
     5401 ttaacgtcta tagtgccgtt tatcacatct ccgtgagagg tcttcttgtc tatcatgaac
     5461 gggtcaacgt cacgcacaag gctgtctttg tcgatgacat aacagcgaac gttggttatc
     5521 tcatacttgc cgtcagagaa attcacctta acgctgtctg cactatagtc ctgacctgtg
     5581 gttatgacat attcaaaacg tctgttatta ttgtaatact tccaatcagg tgcagtgagc
     5641 ttgtttttat tgccgtttat ggacacccaa gcgtcctttc tgccaccctt tatatcgttg
     5701 ttcacattga aagatacaaa cattatctca ttgttcgtga gcgtttcggg aagcgttatc
     5761 tcctgagtga aaggcttttt agcgtcaatg gtgtattttc cccattcacg ctttatctgg
     5821 tcgcactctg taaagttgat ggagcgtacc tttttgatat cagatgaaaa tgacttttcc
     5881 gtcttatcct caactattat attattgaaa agagcttcca ctgagtaagg ataattcagg
     5941 gaattatatt cttttctgcc gataagagca ttggtagcat atccgaaagg cagaacgtca
     6001 ttgttttcat aaagtgaaag gcttccgctc tcttttatct tcttgtagcc tgacactgcc
     6061 tttaccgggt cgtcagtgat aagatacctg tttgaaaaat acatatcagc aataaggctt
     6121 cgtgtctgag tggtcaggga agtgttgcgg tatgaatttt cgttgtatat ctcgctgtaa
     6181 taaaatttgt tgtaatcttt attgtgcagt gaggaatata ttgacatggt gtaataatct
     6241 gcattatata tcatattcac tgtgttcact tcgtcaacgc agtttgaagt tctcgatatc
     6301 tcctcttgcg agccgacata atctgcaagt gtattcatat cactgctgtt atcatattca
     6361 agctctctta aaggcacaag ctcgtctgca aagtttgcat atactgtgga taccacgagg
     6421 caaagggaca ttcctatatt aagtatgaac tcttttttgc aacgtctgta tacaaacagg
     6481 ctcgacataa gcacaacaaa gtcaaggata atataaaact ccactttttt ggtggtcttg
     6541 aaccagaaaa ctcctgccaa tgcaactaaa agggttatca ctgcaagtgg tttaagcttt
     6601 agctttcctc ttattatatc aaaataggtg tgtccgaaaa ggagcatacc aagaggaagg
     6661 aaaggtataa gaactttcgg gtcaacatac agtgtgccgt ttaacatata gactattata
     6721 gggcaggtgg ctattaccat cataacgatt ccgagaaatc ttctatagcg ttgttttgag
     6781 aacaccgccg atattatgct aagcactgtg aaaaggcaaa gccccattga gtagtgataa
     6841 tagagcagga atttcaagtt tacgcctggt atgaacgact tgagatcaac atggctgttg
     6901 cctgcctctc tgcctgagag catacagtgc agtgtcggca ggataagcac acacgccatt
     6961 atcactgcta gaataagcct gaaagcgaat gctgttccgt ctttgcagaa cttcttgaaa
     7021 gttggctttt catttatttt cagccagcgg taaacgccat acactgccat tgccgcaaga
     7081 cccgacactg caaagaaata gcttgtcatg agcatcaaaa atgcccaaag cgtcaccatg
     7141 tattttctct tgccctcaaa ataatcctcg atagccataa aacctaaaag caggaacggc
     7201 atataattca cgaacattat gtgcctgtga ctgtggaaga taaggggcgc tgaaaagaga
     7261 aacacaaagc tcagtgcaaa cgctgtgttg tctgtgaatt tctgacgcat ccatatatag
     7321 aatatccaca ggcttgctat aatgcctatt atggatacta tctgtatata ggtggacatt
     7381 ttcacaaagg gcagaagata agaaaaaagt attataggcg aataaagtcc ataatatgac
     7441 agattataga tgttctcacc acaacctatg ttcggagcaa aggagggaaa aaagtcccct
     7501 gttttgtaaa agagctttct gaaataatca gggatagcat agtgctgtcc gccccaatca
     7561 agctcagagc cataggcgta tttaaacctt gtgagcacaa ggacaaacag aagatatatc
     7621 cctgtaagca cacacagatg tattatattt ttcttactga gcttcattca cagccactcc
     7681 cagcgcagtt gcagtttgtt ttttcatttc agggtgttca ctgtagcgtt tatacagtgt
     7741 ccaatcctca taataagcca tatgccacag cttgtctaca gttttctcct gagaggtata
     7801 catatccgca agctttttct tgaactcgta tctctctttt gatatctttg gcaaagtgtt
     7861 gcccaccact ttcagacggc tagccttgta atacttgcca aagcagtaaa ggtcgcactg
     7921 gatatcgttc ttatcgtata tctcagtgac gtaattatgc acgtttacat ggtggatatg
     7981 accatattcg cctttttgat tatgcacagc tataagcttc caatccttgc aggtcattat
     8041 cttttcaagg tcagattcga taccgtcctt aacaagcgcc caatcgtcac gcttacccct
     8101 gactttatca ggatattcaa gcattataca ctcgttgccc gaggcagtca ccacgtcctt
     8161 gaactcctga gaacgcacct tatttcttcc gtttgttaca cacacaacaa gatagccttt
     8221 atcataaaga tgtcctccgc cccaaagcac ttcgtcatca gggtgagcca ctatcataag
     8281 cttatcagcc tttgatgacg aagcaagctg taactgtata gcggatatag gcgtaacgtc
     8341 ataagcctga ttgatcgtat ctcttatatg ccttttgtag cctaaaaacc acagtgtgcc
     8401 tataaaaatg actattgcca gaattattgc tgtttccttt atatgctgtc ttgtaaatct
     8461 gagcataaaa atgcccgcac ccttgcagga tttcttctgc gagatacggg cgttcacctc
     8521 tttctttatt acttgttgtt cacatagtct ataaatcttt caaatgcagc ttttccgtct
     8581 ttgtttctct tataaacgcc tgcatgttca agcacctttg cgaacacgat gcctatttct
     8641 ttctgcaaga cctcgtgaac gttctcggct gttatgttgt ttttctttga tatctcctca
     8701 gcccagtcag cgtgcttttc tatcagcttg tcacggcgta caccctctgt tccctctgtg
     8761 accagaacct gagcaagctt ctccatttcg cctttaagtc ttgacggaag cactgcaaga
     8821 cccattactt cgataagacc gatattttct ttctttatat gatgaagctc ctgatgcggg
     8881 tgataaacgc caagaggata ttcctcggtg gtgatgttgt ttctaagcac aaggtcaagc
     8941 tcatatctgc cgtttctcat tcttgcgata ggggtgatag tgttgtgcgg ctcgccttca
     9001 gtttcagcgt aaatgaacgc ttcttcgtcg gtgtagcctc tccatgcttt gagtatcttg
     9061 tcagcaaggt caacgagagc gtttttatcc tcgctgttta ttctgataac ggacataggc
     9121 catttaacga tacctgcgtc aacgttctca aaacccttga aaactatctt ttcttcaaca
     9181 ggggctttcg ccattgcaaa agtataatga ccgccctgaa aatgttcgtg cgaaaggata
     9241 gatccgccaa cgataggcag gtcagcgttt gaacctatga aatagtgcgg aaactgtctg
     9301 acaaagtcaa aaagcttctc aaatgccgaa cggtctatta ccataggagt atgcttctta
     9361 ttgaaaagga tacaatgctc gttgtaataa acatatgggg aatactgaag tccccagtct
     9421 gtgtcgttta ttttaaggga tataacacgg tggttctgtc ttgcagggga gttcactcgt
     9481 cctgcatatc ccacgttctc atagcacagc aggcacttag gatatcccga ctgctttgca
     9541 agctttgccg ccgcaatggc tttcgggtct ttttcaggct tggaaagatt aatggttata
     9601 tcaaggtcgc cgtactctgt tggagctatc cagcgcatat ccttctttac tctgtatctt
     9661 cttatgtagt ccgtgtcctg cgaaagcttg tagaaatagt cagttgcgac ctttgcagac
     9721 tggtggttgt aaagtcccca gaactttgtg tctacctctg acggtcttgg agtaagaagc
     9781 cccatgagtt ttgtgtcgaa aagatctctg tatacaatag aattttctgt aaggcccttt
     9841 tccacagcgt aatcgagaag ctctttgaga gtgctttcaa gatcaacgtc agaaaactcc
     9901 tccgctggtt cgtcatattc ctcaaggtca agggcttcaa gaagcctgtt tgtggtataa
     9961 actctgtcgc tctcctcaat aagtccgctt tccagaccat atgttacaag ctttttaatg
    10021 ctttcaaata tcatgttata gatccccttt tgtacaaaag tatgaggagg aaccccctca
    10081 gccagcatct attattatag accattgctt attctttgtc aaccgacaga caggctaatt
    10141 aagatacttt taagcatttg aaaagcgtaa taagaatatt ttaagattat tttgcagggg
    10201 acgccagccc ctacattgaa gttagggaaa acgatttagc accgaatatt aacaaattgt
    10261 taaatcggca attgtcctat tgacaaaagc gggaagtatg acaaaggcgt tgacaggaac
    10321 aaaagcaaag ttctgacatt acagagagcc gtcggtgggt gcaagacggt atgcgaaatt
    10381 tgtgataact cgtccccgag cctgaacggt gaaatcttat tagccgctca cggattctcc
    10441 cgttatagag atacgcattg atagatgcgg ctgagatatg cggtttgagc cgtatatgaa
    10501 tgagagtggt aacacggcaa agctcgtctc tcctttttgg taggagtggc gattttttat
    10561 gcccttttcc acatcactat caaaattatt atcggcttta tatttataag gaggatctga
    10621 catgaaaaac gtagaaaagt atgagagagg ctattatatg cctcctgtaa agtgcatgaa
    10681 gtgggctgaa aaagagtatg ttgaccacgc tcctgcatgg tgttctgttg acctgagaga
    10741 tggaaatcag gctcttatca ttcctatgag ccttgaagaa aagctggagt ttttcacaaa
    10801 gctcgttgag ataggtttta aggagataga gataggtttc cctgccgcct ctgaaacaga
    10861 gtatgagttc tgccgcacac ttattgagaa gaacatgata cctgatgatg ttactattca
    10921 ggtgctcaca cagtcaagag agcatatcat aaagaaaaca tttgaagcta tcgacggcgc
    10981 aaagaacgct gttgtccacc tttataactc aacatctgtt gcacagagag agcaggtttt
    11041 ccgaaagtct aaggaagaga taatcaagat agctacagac ggtgcaaagc tctgtaacga
    11101 gtataagaag cacgcaaagg gcaatatcgt atttgagtat tctcctgaga gcttcacagg
    11161 cacagagcct gagtttgcaa gagatatctg caacgctgtt atcgacatct ggcagcctac
    11221 acctgataac aaggtcatca tcaacctgcc tgtaacagtt gaaatgtcaa tgcctcatgt
    11281 ttatgctcag caggtagagt atatgtgcga cactcttcac aacagagaga acgttatagt
    11341 ttcacttcac ccacataatg acagaggctg tgcagtggca gactgcgaaa tgggtctgct
    11401 tgcaggtgct gacagaatag agggtacttg tttcggcaat ggtgagagaa caggcaatgc
    11461 cgatatcgtt actattgcac ttaatatgta cactcacgga attgacccta agnnnnnnnn
    11521 nntcaggcgc acttgtattc gctgcattct caggctcaca tcaggacgct atcgcaaagg
    11581 gcatgaactg gagaaaagaa aagagctgtg acaagtggac agttccttat cttcctattg
    11641 atccgcatga cgttggcaga gagtatgacg gtgacgttat cagaataaac tcacagtcag
    11701 gcaagggagg aataggcttt atcctccaaa atcagttcgg ctatgatctt ccaaagaaga
    11761 tgagagagga ctttggctac aaggtaaagg ctgtttcaga ccacaagcac aaggagctta
    11821 tgcctgatga ggtctacaag atattcaagg acgagtacct caacaagtgc gcacctgttg
    11881 atattcctga agcacactat gttcagaagc agaatggcac tatctgcgcc gctattacag
    11941 cttcagtaaa cggcgtaaca gacgatcata cagcagaggg taacggacgt cttgactctg
    12001 tggcaaatgc tatcaagcag gctttgaact ttgattttac cgtggaaaca tacacagaac
    12061 acgctcttga aagaaagtca acatctgagg ctgtttcgta tgttggaatt tcctgcggtg
    12121 ataagatgta ttggggcgtc ggcagacact cagatatcat cgtatcttct atcaagggtc
    12181 ttgtttcagc tatcaacaac gcttttgctg acaacaagta ataaataaaa tttaagggac
    12241 aggcgaaagt ctgtcccttt ttttgtacaa aaaatgcagc catatagaat atgaccgcat
    12301 tatatattat ttcttttttc ttctgaaaag ccttttgagc cagcctaaaa agcccttttt
    12361 ctccttttca ggttcgacag cctttgaatc attaacagga gcaggctttg gtgcattggc
    12421 gtatgcttca gcgtaaaatc tgctttgtgc gtcaatggcg atatctccac gctctgcacg
    12481 gacatatttt ccgtcaggct gcattatcct tgcttttacg ttgtcctgca tctgggtgtc
    12541 aaaaatatcg aggacacgtt tttttacttc ttcactgagg ataggtgcgg cgacctcaac
    12601 tcgccttgtg gtgtttcttg tcatatagtc tgcgctggat atgtatacct ttttgcgaac
    12661 acctgtgccg aaaatatata tccttgcgnn nnnnnnnntt gcgtgttcaa gatatctgcc
    12721 cactattgag cgtatctgca cattgtcagt aacgccctcc acacctgcaa caaggcagga
    12781 tatacctctt ataacaagct ctaccttcac gcctgcctga gaacattcta ttatcttgtc
    12841 cataagcacc ttatcagtaa gagaattgag ctttgcggca acatatccct caccgcctgc
    12901 tcttgcaatg cttatctcat tatccataag ctccactacc ttattttgca gacagtgggg
    12961 agcgaccata agtgcctttg agctttccac aagctcgccc attgacagcg ttctgaacac
    13021 ctcgcaagca tcctgagcaa tgtcattgtt tgatgtcata aggcaaaggt cggtataaag
    13081 ccctgcggtc ttttcgttgt agtttcctgt acccacctga acagtgtact ttacgctgtc
    13141 accaactttt cttgtaataa gacaaagctt tgaatggact ttaagtccct gcggaccata
    13201 cattacatga cagcctgact cctccaacag ctttgaccac cctatattgt tttcctcatc
    13261 aaaacgtgct ctcagctcta caagcacaag cacgtccttg ccattttctg cggcgttgca
    13321 caacgccttt atgaccttgg agttcttcgc cacacgatac aaagtcattt tgacagacgt
    13381 aacgctatcg tcctttgccg cctcgtcaag aagcctgata aaaggcttta tggactcata
    13441 aggatagtgc agaaaaagat ccttttcgtc tatctgctct atcattgacc ttgacgggtc
    13501 tatcatcgca gagggctgag gctcctgctt cgggaaaaaa agctcttttc tgtctgccgc
    13561 cttgtcaaac acagcgaaca cataggacat atcaagggga gttttctcca caaagacttg
    13621 tttttctgaa agctccaacc ttgaaagcag ttcgttcagc acagtgtcgg atatttgctt
    13681 tgaaagctgc aatctcacag ggcaaagcct tttacgcttg ttgataagct cagacatatt
    13741 ttgtctgaaa tcaagttccg agtcaaagcc ctcgtcaacg tcgatatcgg cgtttcgtgt
    13801 cactctcata agagcctttt cttctatttt atagccctca aatgccttgt cagcataatg
    13861 gagaataagc tcttctacaa ggatatagct tatgcacccc tcgtcatcag gaagaaagat
    13921 cacacgctgg aatttctcgt tacagctaac tatacccact gtgacggcag attttgaagc
    13981 gagttttgcg accgcataaa tactttgatt tgcaagaaac gggaaagggt gtcttttatc
    14041 aatgataaac gcattaagaa acggcttgac ctcataggca taataggctt caagaaattt
    14101 cttatcctgt tcagtgagag cctttatgtt tttatgtact atatttttaa gctccagttc
    14161 ctcgcagata gctttgtatg tcttatcttt tattggtata agctcatgaa cacgctcaaa
    14221 tattgcagaa agctgttcag atggacgcat tctggacttg ttgtctttat gatcgtcgtc
    14281 cacaagcgag gaatcataaa gtgaacccac acgcacccta aaaaactcgt caagattgct
    14341 tgcaaatatc gactgaaaaa gcaatctttc aagaagcggt gtagcactgt cctgagcttc
    14401 ttcaagaact ctctcgttaa atttaagcca tgataactct ctgttatcgt agaccttgtt
    14461 attcatattc ttatttctcc taactcctat cgtaagaaag gcatcttata tcatactgta
    14521 ttattatacc gccaaacgaa tatgatgtca atgcaggtgc cgtaaaaagc ttgtaaaatc
    14581 gccataaaac aaaaaaactc ccgcccgaag gcaggagcag atcgtcttga agatcagatc
    14641 ccgatatatt ccttagcggt ctggcatata tttgcacagt tattcgcaag gacgctgttg
    14701 tcttgtggca gattcggcaa catgataagc caaaaaagaa tgaacaccat tataagcata
    14761 agcgttgcac cagtcaaaag tatcaaagcg gctgaacgct atgatttcag tgttgtatgt
    14821 acgtcgctgc acactttttc ggcgggctgt gcattttctt tttcctcgtt agaaatacgt
    14881 ttttcttgct cttttttaca ctcagtacac aacatatact ttaaacctcc ataatctatt
    14941 tttacttcat taactattat accacattct taccgtcttg tgttaagctg gcagctataa
    15001 tttacaaaat tttgactgaa ctttttgtta atatgtaaaa ttgtaaaact tgtcatgtga
    15061 ata
//
LOCUS       DAWEMR010000050        14964 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1658_length_14964_cov_11.716145,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000050 DAWEMR010000000
VERSION     DAWEMR010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14964)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14964)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14964
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1658_length_14964_cov_11.716145"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7787..7796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13650..13659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4148 a         3661 c         3228 g         3907 t
ORIGIN      
        1 atgcaagctg ctttttcaac ttacagaaaa tgttgcatga ataaagaaac tttatatcct
       61 gcattttgtt catattcaca aatacaagaa aagaggggcg ctccaaagca gaagcacccc
      121 acaaatgaag atttggtaat cagtagaaag cttagtcctc aaacatcttt cttgcagcga
      181 agcatatcat ctcggcagtg agatcagtgc cgtattcgct tacgtcgatc ataagatgtt
      241 tgttgtcacg gtcaccacga tttttgcctg tgacgtattt ataatagttg tggcgctggc
      301 ggtcaacacg ttttatgagc ttgtcagcct ccggccatga aaggtcataa gtttcacaca
      361 tatgctttat ccttgcttcc aaaggcgcat aaagaaaaac gttcatgtgg tttggatatt
      421 ctctgagaac atagtctgca catctgccaa agaatatggc ggatacacgc ttgtttgcaa
      481 gatctctgat gatatccacc tgcgctttta cagccttgtc ggaataaaac tgagttgatg
      541 agccgtaacc gaatgacttc agcaggcggt cagcgttctt gtcaagcatt tcctcactgt
      601 caagaaacga gtcacggctt atgcctgcac gctccgccgc catatctaca aggtccttgt
      661 cataaaactc cacgccaagc tcagccgccg cctttcttgc gacctctctt gcgttgcagc
      721 cgaactcacg gcttatggta ataataaact ttttcatagt ctatgctcct tttttattta
      781 tgagattttc tttcgtggcg gctatcctcc gctcctgtct ggcttttatc tggatcttcg
      841 gcaaattcca accgaatttc accgacatga gccttaggga aaacgtcacc acaaggctca
      901 ccatgcaggc aggcatgaac gacatcatgc catagcaaag cagaaaagcc gctgcacctg
      961 tggcagaagc gcaggcataa atgttgccca caaatatctc aggcttcttg ttcacgaaaa
     1021 tatccctgag aactccgccc ccaacgcccg ttataactcc cgaaaacaca agcaaaaaag
     1081 tgttgtctga cacgccgctc ctcattgccg ctgttatacc tgtaacagtg aaaatgccaa
     1141 gccctatagt atcgagcatg agcattgcag tatcataaag cttcaacgcc ttgcccccca
     1201 ggtcgccttt tacagccaag gctgcaaaca caagcaccga tatcaaagcc gccgcactca
     1261 ttgcaagggg gtcacgaaac gccgccacag ggtgacttcc gatgacgata tcacgcatta
     1321 tcccaccgcc cacagccgtt gtcactccta ttattataac gccgaaaagg tcaaaacgtc
     1381 tttttatcgc tgtcagcgca ccgcttgccg caaacgccgc agtgccgata agttcaagta
     1441 tcgtaacgat aacatcaaaa cgctcattca ttcaacgtcc ctgcctgcat atttgtgtat
     1501 cttccgcacc gccgtcgcct gacttatgcc aagcttctga gccacagcca cagatgttct
     1561 gtatttttca taggctctca tcacaagtcc gctttcgtaa aattcaagca tatcctttag
     1621 ggaagcattc tgtgcaaata cctcagacga accgcctgtt atcctgtcag gcaagtcgaa
     1681 aacgtccacc tttttcttct cggtagtaag aacgatacgc tcgatagtcc tatgcacctc
     1741 gtcgatattt tctttccaat catatcccaa caaacagccc atggcacgag gtgtgaactc
     1801 aacgctccgt gagtatttct tgtttgccga gccgagataa tagtcgataa agcttttaag
     1861 atcctccgca cgctctccaa gagaatacat ctgcacctga taaggctcga actcgcttat
     1921 aaactcctcc gacagcttgt cagcaccttt aaactcttcc accgaatgtt cagaaagtcc
     1981 cacaggcaca atgcctgctt ttgcgcattt ttcaaggact gccctttgaa gcctaggcga
     2041 cagcctgtct atatgcttta ttatcacagc gtaaatgccc gaaagcggcg atatcatcga
     2101 cccctctcca agaagctcct tttctatgac ttcttccccg acaggtgcgc agtcataggt
     2161 gtaaagcgtt ccttttttgc cgtatatagt ggaagcaaga tagcttttgc cgcttcctgt
     2221 gggaccatag atgtaacacg gacagcctgc gtcacagaaa actcctgcaa gccgccttgc
     2281 gtctcttgaa cggctgcttt gggcgatata acgctgtccg ccgtcacgtc ctgtcataag
     2341 ctctatttca tcaaggagct ttttgttctg atactgcaaa acattgtaac gctcccgcag
     2401 gtcgtcaaag cctgttatct cccaagagga atagcttatt atcatctcaa gctctccgtc
     2461 cttgaatacc ggtattcccg tgacgaaagt ttcctcattg tcaacgcctt tttgcactgt
     2521 tgtgacacgc ttgtggcttt caaaaaccat ttgcgtcacg caaggggaaa acatcttcat
     2581 catttcctct gagcctgcga atttacctat tatgtcagag ccttttacgc ttctgccgct
     2641 gagataggag tttgaaacaa atctgaacac tccgctgctg tcggcaatag tcactcctgg
     2701 gtggatactt tcgagaatgt gccctagctg ctcataatcc atttgtctgc actcctttct
     2761 gcggctttat agtttacaca ttaagtatct ttgagaagaa ctgctttgca cggtcgctct
     2821 tagggtgctt gaacacttcc tcaggagtgc cgtcctcgat aacatagccg ccgtccataa
     2881 agcagactct gtttgctgcc tctgccgcaa agcccatttc gtgtgtgaca actatcattg
     2941 tcattcctgc ctgagccagg tctttcataa cgttcagaac ttcgccaacg aactcagggt
     3001 caagggcgga agtcggctcg tcaaagagta tgacctcggg ctccattgca agggtcctcg
     3061 caatagccac acgctgctgc tgaccgcctg aaagctgagt aggataagcg tctgccttgc
     3121 cgtccattcc aactctcgca agaagctcca tggctttctt ctttgtttct tccttgttag
     3181 cctttttaac ctgaacagga cactccatga cgttttcaag cactgttttg tgcggccaaa
     3241 ggttgaactg ctggaacacc attccaacgt tctttcttat gacctttgca gccttttcgt
     3301 tgagcttttt gttgtgcttg tcctcgtcga tgccctttag ggaaactccg cctatatcta
     3361 tctcgcctga tgtcagctcc tcgagatagt tcaggcatct gagaaaagtg cttttgccgg
     3421 agccgctagg gccgataacg cttaccacct cgcctttgta aatatccata tctatttttc
     3481 tgaggacctt aagctccccg taattctttg ccagatcgtg aaccgatata acaggctttc
     3541 tgctttcagc cattgtattt tacactcctt tttcgtcaga tagcaacacg gttgttgtgc
     3601 ttttctatac atcttgatgc gaactcaata acaaggttca gcgcccagta aagaacgcct
     3661 gcacagatat aaactatcag agggctgaaa gttataccga tagcctcttt tgtcatcatt
     3721 gtcatttctg caactgtgat gatagaaagg atagatgagt ccttaacagt ctgaacaaac
     3781 tgtcctgtca gtgacggaac gattattttc aaagtctgcg ggaaaattat tctcaccatt
     3841 gttttaaatc ttgacattcc cattgcatat gaagcttcaa actgtccctt tgggatagcg
     3901 ctgacgccgc tttcaaggat aactgcggta taagccgctg tgtacattcc aagaattata
     3961 acgcctgtcg agaacgctga aacgttcaga ccaagagcag ggagtccgta atatgcgaga
     4021 tatacctgca ccataaaagg ggtgcatctg aatatcttta taaacgccct tgcgataagc
     4081 tcaacagcct tgttttttga aaggcacata taacctaccg ccgagcctaa caccattgca
     4141 ataaggatag acagcaccga tattgccgcc gttgttccaa gaccctgcaa aagcagcgga
     4201 agcgctattt ctgcaacgtg cataaattcg tccatattat tgcgcctcct tatcttcttt
     4261 caagtctttt cttctgaagc tttacaagtt cttcaagtcc tgcgcagata acaaaataca
     4321 taactgcgat agcaatatag aaaggcaggt ttatgtatgt ctgcgtaaca agacgctgag
     4381 ccgttcttgt aagctctacg attgccaata cagaaagcat cggcgaaacc tttataacgt
     4441 ttatgaactc gcttgtcagc tgcggcaaag ctaccttgaa tacctgtggg aaaactatcc
     4501 tcattagtat ctttggctcg ctcatgccca aggctctggc cgcctccgtc tgaccttttg
     4561 gcacagccgt aagtccgcca cggattattt ctgcgataaa tccgccgctg ttaaggctaa
     4621 gagcgattat gcctgacgcc aggtttgaaa ggttcagacc cattactgca agcgcctgat
     4681 atataatgta tatctgcacc agcacaggcg tgccacggac aaagcttata taaactgcga
     4741 ctatcttttt cagtatcctt gaaggaagcg agtcgccgcc cattctcatc accgaaagca
     4801 gcataccaac gcaaagcgct ataagtatcg ctgcaacgga tacgaatact gtatagccaa
     4861 gacctttcag cagcaggtct ttataatgcc atactatgtt gaaattctcc attgatatat
     4921 aatgctccgc cgtttgcgac ggagcatttc ctcctttcaa aggtgctttt tactcggcag
     4981 ggatataatc gctctcaggc aggtcaacgg cttcaccaaa ccatttcttc tggagctctg
     5041 caagagttcc gtcctctttg aacttgtgta tctcagagtt gatatagtca gaaagctctg
     5101 tgtcttcctt acgaacagcc catgataccc aagtgtctgt accgaccatt tcagccttgc
     5161 ctgaatcgtc agccactatc tcataatcgt caggattctt cattacaact gcggcgcagc
     5221 ttgcatagtt ctgagcaaca aggtctatct tgccgttctt aagctctgca aaagcctcag
     5281 ggaatgagtc gtatttgagc agttcagcat agccgtcctt gccctccgac tttagcttct
     5341 cgttgaattt ctttgtatcg tcctcattgt agcaagaagt ctgtgtgccg acctttttgc
     5401 ccgacatatc ttcgatagac ttgatgctgt catcgccctt tctctttacg aaaactgtag
     5461 ttccgttctg gataggataa gtcatcgtat acttgcctgc acgttcagca gttacgccga
     5521 gtgttgctcc aacaacgtca tatttctttg cctcaagtcc tgcaagtatt ccttgaaacg
     5581 gcaggtcgat ataatcaagc tctaccccca gatcgtcgca aatcttagcg aacagatctg
     5641 cgttgtagcc tatgatctta tcgccgtcca tgtattcaaa aggctcataa gcggcttctg
     5701 tcgcaactgt gagcttaccg ttttccttga tctggtccag tgtgagcggt cctgtgttct
     5761 tgctgcttcc gcaggctgta agacatcctg ccgcaagtac tgaagccact gccactgcac
     5821 aaacttttct taacattcca aatcttcttc cgcttttcat aactgattcc aacctttcac
     5881 ttacttttct aaataattcg gagcgctgac aagccggaca ccctagtgcc gccgtcaacg
     5941 ctccgttgcg tttgatatta tatttattgg aaggtgaatt atttttctat ctttattttc
     6001 tcttgccgat cttcttctga taagcaagga tagacatgaa ctcaaaaatg atatttgcgc
     6061 ccatataagc tgttatctct gagtggtcat atggaggagc aacttctact acatcaaagc
     6121 ctgtaaagtt caggtctttc agcgctctta tatattgaag tgtttcaaag gaagtaaatc
     6181 cgcccacctc aggagtacct gtacctggtg cataagccgg gtcaacaaag tcgatatcga
     6241 acgtaaggaa caccttattg tcgcccacct tttcctttat gaccttgaga agatcttcag
     6301 gagaatatgt gtggagcttg tcccctggga taaccgtgaa gccaaggtct gcggcgatct
     6361 tgaaatcgtc aggatcgtaa agagaacctc tcatgcctat ctgaaccgac ttctcaggat
     6421 caataagacc ctcctccatt gctcttctga atggtgtgcc gtgattgtat ttctgaccga
     6481 aaaccgtatc gcacaggtca aggtgtgaat cgaaatgcac aaggcttacc tttccatgct
     6541 tctttgcgat agctctaagt tcgccaaggg tgatagcgtg gtcgccgcca atgcttatcg
     6601 gaactgcacc gcattcaaga acaccgtcca aggtctgctc gatagctgca tatgtttcgt
     6661 ggatataatc agggatcaca ggaacgtcgc caaggtctgc gcccttgagt gaatccataa
     6721 tattgacgcc cattgcaaca ttgttaggct ttatcattgc cgagatgttt cttacaccgt
     6781 taggaccaaa tcttgcgcct gctctgaaag aagcggcggt atcgaacggt ataccaacga
     6841 tagcgaaatc caatccctct gctgaggtcg cgtgaggaag cctcatgaaa ttacccatat
     6901 tacaaaaacg aggagagcct gaggcactcg gtggctgaca attgttgttg atcaattgag
     6961 gtcactttcc tttcaaaatc actgaggaac atatccgcat gaccgttcaa gtcatttctg
     7021 aattgctcta tgtgcagaat tatatcacca ttcctgcaag ttgtcaagag ccatttttta
     7081 atttttctga aaattattca ctttggaata agctttgtta tcacttatat agcgtgttta
     7141 cgccatttcg cccatatcaa aatcgaaact attcattttt gaatagtttc tttgtgcaaa
     7201 tctacaaatg gcaataatgc aatattcaga tatcagactt tcattttgtt cacatctatt
     7261 ttttattcaa agctgaataa aataagaatt tttcattaac actattgcca aataaaaagc
     7321 tctgtgctat actgataatc aaagcaatga cgctcttata accgatacgg caatgcctgt
     7381 cggtttataa gggcgttgtt tttttattat tactaggtca aactcagaaa acaattttga
     7441 aaggaagttt tattatggct tgttttttag taccagcaac agaagcgatc gttacaacta
     7501 ttgtggcaaa ggcagtaaag tctcacgagg caaagacaga ggagcagaca aaggtcaaaa
     7561 ctctttcaga ggacaagctt cctctttcta aaaagataac aaggctctca ggctttctgt
     7621 ggggcggctc aggacttttg gcatttgaac atctgtggca tggcgagata tctccgttct
     7681 tcccattcct gacagcggct aacgaccctt cggaaatggc agtagcattc cacgaaatgg
     7741 cgactgtcgg aacatcaatg gctgttcttg tgacagcggt atgggcnnnn nnnnnnctgt
     7801 actgactgtg gtgtctgaca agatactgaa acgtaagcca aaagagactg caaagctggg
     7861 aggtgatgcc gtatgacact tcttatcaca ctcatagcag ccgttatcgt tactatagta
     7921 tggtacacat caccaaaagc cagaaagctt aaagtggggc tgatgtgcta tatgtactgg
     7981 ggcgcatcta taatgtggct cgtggacgca ctttttgaat acaaagagca gggcgcagga
     8041 tatttcaccc ctgccgccgc agacatgata aacgacagct ttttagggat ctgcgttgtt
     8101 gcactcggca ccgtcgtatg gctcggcact atgctcataa gcgaccctga gggagtagta
     8161 agaaaagtcc ttacagagaa aaagcagtag atacgctata ctatataaat aggaggaata
     8221 ataatgcgac ttgttccaag agagcaggat aagcttatgc tccattatgc gggcatgctt
     8281 gcccgtgacc gaaaggcaca gggattaaag ctcaactacc ctgaagctgt ggcatacata
     8341 agcatggaag ttatggaaaa agcaagagca ggagcgtcag ccgctgagct tatgcaatac
     8401 ggcacaaagc ttcttaccgc tgatgacgtc atggacggcg ttcctgaaat gatacacgaa
     8461 atacaaatag aatccacaat gcccgacggc acaaagcttg tgacagtaca caaccctatc
     8521 aagggtgcaa gcaagcttca cccaggggaa tttatcgttg aggaaggcac tgtcaagctc
     8581 aacgagggaa cagaaagcat tgagcttaca gtttcaaaca caggggacag acctatccag
     8641 acaggctccc acttccactt ttttgaggta aacaaggctt tggaatttga ccgcaaggtg
     8701 gcttatggta tgcgacttga tatccccgca ggcacagcgg taagatttga gccgggagag
     8761 aaaaagaccg tccgtcttat acctatcggc ggcgaccgca taggctatgg tctgaatggc
     8821 cttgtaaacg gaaaaatgga cgatgaaaac atcaagcagg cagcctttga aaaggctaaa
     8881 aagcttggct tcaagggggt gtaggaaatg atcgagatat cgagaaaagc ttactgcgat
     8941 atgtatggtc cgacagttgg cgacagggtg cgcttgggcg acacttctct tattgcagaa
     9001 gtggaaaaag atttgacagt tcatggcgat gaagcaaagt tcggtggagg aaaatctctc
     9061 cgtgacggca tggggcaatc ctgcaaagcc acagacaagg agtgccttga cactgttatc
     9121 acaaacgctc ttatcatcga cagcacaggc atcataaagg ctgacgttgg cataaaggac
     9181 ggcaggatag caggaatagg caaagcaggc aaccccgaca tcatgaatgg cgttgacaaa
     9241 aacatgataa taggcgcttc cacggaagct atcgcaggcg agggacttat cctcaccgct
     9301 ggcggacttg acacgcacat tcattttatc tctccaactc aggtgagaac agccctttac
     9361 agcggaatca ccaccatgat aggcggtggt acaggtcctg ctgacggcac caatgccacc
     9421 acctgtacac ctggcgagtt caatatccac agaatgttgg aagcctccga agacctgcca
     9481 atgaatttcg gctatctctg caagggaaat tcaagcgaca tcacaactct cgaggagcag
     9541 ataaaggcag gctgtatagg cttgaagata cacgaggact ggggctctac ccctgacgtt
     9601 atcgaccacg cacttactgt tgcggatatg tatgacgttc aggcggctat ccacaccgac
     9661 actcttaacg agggcggatt tgttgaagat accgtcaatg ctttcaaggg caggactatc
     9721 cacacttatc acacagaggg cgcaggaggc ggacacgctc ctgatattat cagagcggca
     9781 gcgttccaga atgttctccc ttcctcaacg aaccctacaa tgccttacac cgcaaacact
     9841 cttgacgaac atctggatat gctcatggtg tgtcatcacc ttgacaagaa cgtccctgag
     9901 gacattgctt ttgcggattc aaggatacgt cctgaaacta tcgccgcaga ggacgttttg
     9961 cacgatatgg gaatattctc aatgatgagt tccgactctc aggcaatggg acgtgtaggc
    10021 gaagtcatca cacgaacatg gcagaccgct gacaaaatga agaaacaacg tggcgcactc
    10081 ccagaggaca gccagcgaaa cgacaatttc agaataaaac gctacatttc aaaatacact
    10141 atcaaccctg ctataaccca cggcgtatca gaatatgtag gctcagtgga agttggcaag
    10201 gtggcagacc ttgttctttg gaatcctgca tttttcggtg caaagcctga catgataata
    10261 aagggcggat ttattttcgc atcaaaaatg ggcgacgcaa acgcttctat ccctacccct
    10321 cagcctgtgt gttacacaga aatgttcggt ggtcacgggc ttgccctttc aagcacctgt
    10381 atcacgttcg tatcaaaata cgcctacgag gacggcataa aagagaagct tggtcttaaa
    10441 aggcaggtgc ttcctgtgaa gaactgccga aacatctcaa aggctgatat ggtatacaac
    10501 aacgtctgcg gagatataag agtagaccct gagacctaca ctgtcacagt tgacggcgaa
    10561 gttattacct gcgagccttt tgacgagctt gcattggcac agcgttactt tatgttctaa
    10621 agcgaggaaa caaaaatgat agcagaaaaa atattaggca atatctctga aagcaccccc
    10681 tctgtagaag tagacacagt tgagcttgat tggtttgaag ccgagaaaaa gcgcataaga
    10741 aaaaccaccg caggaggact tgacataggc attgccattg acgaaaacct aaaggacggg
    10801 gatatcatct atcttgacga taagctttgc gtagcagtga agctcacaag ctgtcccctt
    10861 atcaagataa acgttcacac tatgcaggaa atgggcaggc tttgctttga gataggcaac
    10921 aggcatttgt cacttaaaat atctgacgac aacgtgctta tcccacatga cgagcctaca
    10981 gaaaagcacc tgaaaaacct cggctttgaa tgcgagaaca tcataggcag atttgacgat
    11041 tttatcgtct gcaaagctca cggacactca cacagccact cccacgagca tggggaacat
    11101 catcaccacg aacattcaca ctaaacggag ctgataaaaa atgaccgata ccgcaggact
    11161 catgcacatt ctgcaaatat gcgacagcct tttcccagtg ggtgcgttca cgctttcaaa
    11221 cgggcttgaa acctacgttc agcatgatat catcacttcc ccaaaagggc ttgaagaata
    11281 tctccattct tacatctcag tgctgcccta caatgagctt ggtgcggcgg cagcggcata
    11341 caatgctgac gaaaaggagc tttgccgtct tgatgagata tactctgcgg cgaaaacacc
    11401 atttgagata cgaagcggct cagaaaaggt cacaaggcgt tttttcaaaa tatgcaacaa
    11461 tggtcaggaa agcttcacag ggctttataa aagcctgacg gagcagggca tatgcaaggg
    11521 tcatcagtgc atcgcctatg ggctttatat gcgtgacaat ggcatcgagc tctcagaggg
    11581 gcttgcggta tacggctatt ccatatgctc cgccatagtc acaaactgcg tgaagcttgt
    11641 acctcttagt cagcttgcag ggcagaaaat tctcggagag agctttgagc ttatatcctc
    11701 cgcttcagaa aaagcccttt tcactgatat aaacgatatc ggcataagcg gcgcaggctt
    11761 tgacctgcgt gcaatgcagc acgaacggct gtattcaaga cagtatatgt cataagctat
    11821 gatttatata atataataag gagtaccaaa aatgagctat gtaaaaatag gcgtaggcgg
    11881 acctgtggga tcgggaaaaa ccgcacttat cgaacgcctt acaagagaaa tgtcaaaaga
    11941 atattccata tgcgttgtaa caaacgatat ctacaccaaa gaggacgcag aatttcttac
    12001 aaaaaactca tgcctgcctg ctgacaggat agttggcgtt gaaacaggcg gctgtcctca
    12061 cactgccata agagaagact gctcaatgaa cctggaagcc gttgacgaaa tggtgaaaaa
    12121 gcaccctgac acgcagatcg ttttcattga aagcggcggc gacaatcttt ccgccacatt
    12181 ctcccctgac cttgcagacg cttctatcta tgttatcgac gttgcacagg gtgaaaagat
    12241 accaagaaaa ggtggccctg gcgtgacacg ttccgacctg cttgttatca acaagacaga
    12301 ccttgcgcca cttgttggag cttcccttga agtaatggct tccgactcaa agcgcatgag
    12361 gggcgacaga ccgtttcttt tcacaaatct catgagccgt gagggcgttt ctcaggtcat
    12421 agactggata aaaaaatcag tgctttttga ggatctgaaa tgaacggcag actaagcctg
    12481 tcaacagcct ttgacggaca aaaaaccgtt actgaggact tatttttcgc tccccctttc
    12541 aaggtgtatt caccatttta tgaccataaa ggctgggcga aatatatctc aatgtgcggc
    12601 tcagcaggag tattggcagg tgacgaaaac gagataaagc tttttgtggg ggaaaactgc
    12661 aaggtcattt tcacagatca gggctatcaa aagcttttca acacaaacgg cggcgtatca
    12721 aaacagagca taaagcttgt tgtgcgcaaa aacgcaagac tttgctatat gccccaccct
    12781 ataatgactt tcacaggctg tgagcatatc tctatgggca aggtgaacat cacagaaagc
    12841 tctgagctta ttttctctga gatatactgc tgtggcagaa ctgcaatggg ggaggagttc
    12901 ggcttgaagc gctttcgctc acgcaccgag atagcaattg acggcagagc ggattttatc
    12961 gacaacactc ttatcgaccc cgccgcactg cccatacggc aaaaaggctt tttcgagggc
    13021 ttcacccaca cgggatttat gtatatctac acccctgaca agacaatgct ggaaagcatt
    13081 taccaagccg actatacagg cgaccctgac agcgcacagc tttgcgccac tcacacggaa
    13141 cgtggcatag ccataagagc cttgggacgt tcgggagaag agatttttga cgcttttcag
    13201 agcattgcag aaaaaatccg cacataaaaa cagcgtataa aatattgtta aactttttat
    13261 aaatgataaa caaaaccagt gaaagcctgc tgtgggctta cactggtttt ggttatttct
    13321 cacgacaata ttcttcgtat gcttcgtcta taagaggtct cagggcgttg tagtcatcgt
    13381 cacctgatat aaagctatag cacggcacac acagatcttt agcaggacaa gtcttaccat
    13441 aatcattaaa atactgctta tcgtaataat ttaccgcaaa gtcacggaca ttgggtttta
    13501 tatcattctc acaaaatgca ccgtccaata tgtacataag atattccgca ccgctcattt
    13561 tgccctcacg gacctgcaca caggattcct cgtcactttc atcaataagc tcgttaaaac
    13621 ccctgtcaat aagccacctt ataaatcatn nnnnnnnnna tatgatttga cgccaacagc
    13681 catatttctt cctgctgttc ctccgtgagc ttgccctcaa cattatttgt tttcctatac
    13741 agcttttcag tatcgtccca atgccaatct gccttatcta tagccatata ctctccccct
    13801 tttgttatat acctttattg tatcacaaac tatcttccat gtcaacattt tccgaatgcc
    13861 tcacaagcac accccctccc cccttgtcaa cccaaacaaa atatgctata atatagggga
    13921 ttgtaaccta caaccatatc aaaatacaga tatatacaga taacgaaagg attagctatg
    13981 gatacttccg cacttctttg gtataagacc cctgcagacg attggaacaa agcactgccc
    14041 cttggaaacg gcaggatagg cgcaatggtg ttctctcagc cacttgaaga gaggattcag
    14101 ctcaatgagg attccgtctg gtcggggggc ttccgtgaaa gaaataataa gtccgcactg
    14161 cctaatctcg aaaaagtgag aaaactgctt tttgaggaga aaataaacga ggctgaaaaa
    14221 ataatctatg acgctttctg cggcacgcct gtaaatcaaa ggcactatat gccacttggc
    14281 gacatgaacg ttatacatta taaagagagc gaatgtgact tcaagagcag aagccttgac
    14341 cttaacaccg ctgtctgcac cacagaatat gccataaacg gcgttgacta cacaagagaa
    14401 atgtttatct cacagcccga tcagatgctt gttatgcaca taacggcttc cgagaaaaaa
    14461 gcgataagcg tccatgtacg cattgacggc agagatgatt attttgatga taactcgccc
    14521 gttcatgaca acgatatcct cttttacggt ggatgtggct cagaggacgg cataaacttt
    14581 gccgcttata taaaggtact ccacaagggc ggaaaggtct acccatatgg aagcttcata
    14641 acctgtgagg actgcgacga ggtgactatc ctccttggcg cacagacaag ctaccgctgt
    14701 gaggattaca agggacaggc tgtctttgac gttgaaagag ccgaggaaaa aacctatgca
    14761 cagcttaaag ctgaccatat cgcagactac aagtcatact atgaccgtgc gaatatctcc
    14821 ctttgcgaca acagctcagg taattccacc ctgcctacag acgaaagact tgcactggtt
    14881 aaagagggta atcctgataa caagcttatg gaaatgtatc acaacttcgg cagatatctt
    14941 cttattgcag gcagccggga aaag
//
LOCUS       DAWEMR010000051        14855 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1670_length_14855_cov_9.981081, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000051 DAWEMR010000000
VERSION     DAWEMR010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14855)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14855)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14855
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1670_length_14855_cov_9.981081"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12658..12667
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14051..14060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4016 a         3231 c         3098 g         4490 t
ORIGIN      
        1 cttggatttt tcaccataaa gtcccgactc aactgttact agcccgtcat tgcccttgcc
       61 gtatggcttg ttgagcagat agccgatatt taacggaaaa cctgcggaac gtatgctgtt
      121 cattttgctc atcacggagc ggtaggaaac caactggctg tcaggggtgc ttgcgttaag
      181 ctcagagcag tttttgtaag tcaggtcata aacgccatct aaaaagcttg ggtctttgtc
      241 acccagagca gtgaaaagct tgttgtattt tctatcaaca aagccctgta tgctctcagg
      301 tatcttacca agaagcatat caacgaattt acagccatag tgtggcgtgt ttatggtggt
      361 aagagttgcc acatatttgt ccatgccgag gtgagagata gcgtatcttg agtcaagccc
      421 gccttttgag tgtgcgatta tgttgacctt ttctgcgcca gtttctgcaa tgacttcttt
      481 tatcctttcg gctacctcag tggcactgac ggatactttg ttggcggact gttgcttgcc
      541 gtaatagacc tctgcgccat ttctaataag ttcattaggt accctgcccc aatagtttat
      601 tacctgccaa tcacggaaga atatgccatg caccataagt ataggatact ttgtcttgca
      661 tatctcgttc tcttttcgtg cagcgtcaag gtcaagcttt gcctgctcga agtagtattc
      721 acttctcgct gccttgtaga attttctgaa cactatgcag ttcacaagcg gtatgtacca
      781 ggtgaataac agaataacat accacagtat tttcacctga cgtgccgaag ccgctatccg
      841 cacaatgcca ctcagacaca aaagcgttgt tatagcataa gcgcaaagtg catttgcaag
      901 aagccgccaa ccgttcagct ttgtaaagca aagcactatc actattataa gctgtataac
      961 gctttgcaaa acaccgcagc caagtacaaa cgctccggaa tcaagccgtc ggatttttcc
     1021 atgagaacct ttgcgtccat gtgatttgaa catgaaaacc aaggtgtata ttgcaaaaag
     1081 tatgatgata agcgttttga gcagagaatt tatgtcaata agtttccaca gtgcaggtgc
     1141 gtttgcaagc agaagcaccg gtatagaaag aaaagttccc ctaatttttt tcatttttta
     1201 ccccttaaag attatttttc agctttacgt gtctaatatc gtttcccaca aacataatag
     1261 gcatgaaaca gcctgtgagc ctcgaaacta tacgctcatt atattttgca ttgtattctt
     1321 cattggtgag attggtagag attatcgttg gcctgccaag gttgatacgc tcgttgatta
     1381 tttcatatat cacggaatcg gtaaagctcg tttgaaattc tgagccgagg tcgtcaagga
     1441 taacaaggtc agcattctct atgatgtcaa gtgtctgacc tgtggctctg ccgaaatgct
     1501 cgtcctctat ttgtctgaga agcttcagca gagaggcgaa aatgacgctg tagccctttt
     1561 gtataagttc atttgcaatg caggctgaaa gaaaagtctt gccaagacct gttttgccaa
     1621 tgaaaaacag tgatggagag ttttcatgaa atttgtcagc atatgcacgg cagtttgaat
     1681 acaccgttct cattttctct ctcggtgaca ttccgttacc gtcgctttca ggataaaatt
     1741 ctattctgaa ctcagagaaa tcgtggagct ggatactgca attctcgtta agctcctcag
     1801 tggtgtattt tttcagcaag tcggtgaagc attggcactt cactccgtca cgatagccat
     1861 aatcacagca tttaggacag taatagtgat aatcaaggta gtcttcagga taacctccca
     1921 ttttaagcag ccctttaatg gcacgtttag tattggcatt gttttgacgc attttttgta
     1981 tatattctct tccatgagca tttttgccta ctatgccctt catgagttca cagttatttg
     2041 cttttagtga tcttgtaaga acctctattt caggaagtcg ctgtgctatc tcctgagcgt
     2101 ggacctcatg ctcgttcatg acagcttcac gccgtctttt aagttcattt tcagccttgt
     2161 catatgcggt tattgaatat ttcattttct actccttatc atagctttcc tgatttttct
     2221 agatcaaaat tcattgcgaa tttttcaaaa tcatcaagat cataggaagt ttgcttattt
     2281 gtattcttat tagtattctt atttttcttg gaggtgtggt acttttcgtc ctcgtgtgtg
     2341 acctgttcag gggtagtgat gctcttgcca gcccagtttg acagtatagt gtctatatat
     2401 ttgtaattca gtttgttggt attgtcaaca cacttgttat aggctatctc cagcatttca
     2461 acagtgaagc ccattgttcg ccacttttct ataaactcaa gctgctgctt tgacggcttt
     2521 gcggtctgtc cgaaaatttt cagtaccttg aattcatact tttttatgtt ggaaagccta
     2581 attatttctt gttcaatttg ttgataagta cagatgtctt gttcaaacca cccacgcatt
     2641 actgtcacaa ggtaagacac gttgtgcttt tcaagtgaca cacagtattc tgcggctaag
     2701 agaatagatg cagcatcaaa tcggtaatat tcataaagag caagaagttc cccttgctca
     2761 gttccattga tagtgcgttg taaaacggtt tgtatctcgt caaaaagatg tgcaaggtta
     2821 gggtccttct gctgtttttc cagtatctcg gaatttttgt atcttatgca cagcttgttt
     2881 gaaacgctct gacgtttcgg ggcaactgcc tcaacttttg aaacaggctc ggcctttgtt
     2941 tcaggctctg aaacaggtgt agaaacggct ttgacctgtg caggatttgc cgtatcactg
     3001 tgttctgtgg tgatgactcc tagtgatacc cagtgtgtca cagcgtcaat gacagtgttc
     3061 tcagaaagcc cggacttttc cgcaagcttt gcactgtcgc attcacgttc cgttgaacag
     3121 agtatgcaga gaagtacctt tacaaagtcg ccatcactta attttatgta tttatcgacc
     3181 actttagacg gcactgaaaa actgcttccc cagcggtcgc aatcaaaagt atatttcatt
     3241 atgtatcctc gcaaatatga ttaaaactat ccaaaataat tatatcacat aatctggact
     3301 tttgcaacaa aatttgtgca tttgactttt ggtaaataaa atgctataat aggctgatac
     3361 caaagaaacg gagagataaa aatgataaga aaatttgtgc ctgaggacag ggaagattac
     3421 atcagatttt ccactgagtt ctataattct tctgcggtgg acaagcctgt tccaagagag
     3481 cattttgagc agggcttcga tgaaatgatg agatctgacg tgtatgtgca gggttatatg
     3541 ctcgtttgtg acggcaataa tgttggctac tgtgttacaa tgaaaacata ttcagttgaa
     3601 gcaggcggta ttactatatg gatagacgag ctttttgtgc tggaggaata ccgctcaaaa
     3661 ggtctgggca gggagctttt taagtatatc gaggaaaatg gcgacaaaaa gctccgccgt
     3721 atccgacttg aagtcgagct tgaaaacggc agagccatat cgctttataa gaaaatgggc
     3781 tttgagcctg cgccttacga cggaatgtgg aaaactattt tatagtcctc tcgtgaattt
     3841 cccttgaaat gctgtcacag accttattaa gggcggcgtg atccattgcg gcgatataat
     3901 acttttctat ctcgtcatgg actttcttgg ctttcttgat gcctgaatac acttcctctg
     3961 caaggtcgga ggcggtgact ttatctaatc tcagtcttgt tttcagggct gagatctttt
     4021 tcttgtcata aaatcttccc atgttgatag ctttttcaaa gccgtcaagc ttgcttatag
     4081 gtgaatttat cacaaatgcg atgccagcct ccgggataag caaatgctcg taaagggtgt
     4141 tgccaaatag gatagcaggg cagaggataa catcataact tcgctttaca gcctgctgtg
     4201 ccatgagggt gacgaacttg tgggaagcgg cataataatc atcgttcatg gcgaacacgt
     4261 caaggtagct gtcaagagtg gcaagctgcg tcatacagcc gtattctgtc aaagcggtca
     4321 actgtctaat atcagttttg ccgctgccag agcctttttt gtttagaaga cgttttgctg
     4381 atcgttctgt gaagcttttc agcttttcct ccagcagaca gccttcaccg caattgtagg
     4441 tatcgaagca aacgttagac agggcagtgg taaacctttt gcttcttgcc aaaagcgact
     4501 tgtttttgtc tgtgacctcg attatttcag atttgaaggt cttcagcttt tcttcgtccc
     4561 aatactctcc taagttgatt attttctgac aaactcctgg atatgaacat tccataacat
     4621 gtggcgcagt gccgtcgcag attatggcct ttgatgtatg cagaacgatt gcatcaaggg
     4681 aagcagggtc agaggaacaa taaaacctgg tgacgtcctc tgtcggttca aactcggcgg
     4741 ctatcttttt cataagtgaa gattttcccg tgcctgcacc tcctttgagt atgaatgtga
     4801 aatactcctt atcagccatt atcttgccaa attccgtgga aaacccattc tgcgtcattg
     4861 cacccaaaaa atacttttct gacataaatt accctccata tctcaaacgc attagatcag
     4921 tgtgactgcg tttgcatttt catttttata atatgtcaga aaaagcatga ttgttcaaaa
     4981 aaattaggtg gacaaaaaag cttgataaaa gtaaaaaaac tttcattgca tgacgttgca
     5041 acttgatatt gtgataacga ctttttcttt cctgcctgca acaggataca aaacgccgtc
     5101 cgctttatgc agtgcctttt ttactattgc agaacttttc attatgttcc gcctttctta
     5161 tctatcttac ttctgcggta tcatacagct agttatgact aactcgtaga cttttatgat
     5221 agcacaacaa ggtggaaatg tcaatagaat tggggcgatg gaaggcggta aggacagaaa
     5281 aataccgata cagagggggc tgtatcggtg gaatatatgg gtaaagctta gtctggaaat
     5341 tcaagagatc ttgtacatat catttttgta gaacattatt gaatcgtttt tttcagagcg
     5401 ccttagaacg gtaaagacat tataaacatc tggaaatggc gaaaccatac ccatacacgc
     5461 cataccaaaa ttcaatccat taaatactct aaattcggca ggactaatta aaaataatat
     5521 aaaaggaacc accccaagaa caaaaggcag cagacacatc aatataaacc tatctcgttt
     5581 taatggatat gatgctaatg ccacaaatgt tgtctttcct ttaattcttc ctattgttac
     5641 acctgcgtcc ttaggataaa cgattccgtg taaccactcg tgaattatca acaataaaaa
     5701 gcctaaagca aatcccacca gaataaatat tggagagatc acgaccacct tgcacacaat
     5761 ggttttacca agcatagcgg caaaaagtaa aacacataga attacagcta tcggcaacgc
     5821 ttttttcatc agttcatcga cgctcttagg cgtttctatc tttattgcat tatcgggtaa
     5881 gtttcctgtt tgatatttat ttatatcatt tattgaccac acaattttta tcatattaca
     5941 tacctttgtt tatctaatct cgatttatcg tctaactata tcgtattatt cattatacca
     6001 caccatatgc cccaaagtca agcaaactat tcgatccgat atagtttgaa actatatcta
     6061 tcccacagaa ataaatattt tactatcgcc ttttttcgtg ctatactaaa taccagcaaa
     6121 ccaataggaa ataaactata gtaaaatgaa agaggtaata aaaatgagca ataatatcaa
     6181 agtaccatgc cgctacgatt ttgtgggcag ctttctcaga cctgactatc tgaaaaaggc
     6241 aagagcagat tttgaaaagg gcgctatcac agtccagcag cttaaagagg tagaggataa
     6301 ggctatcctt gatctcgttg caaagcagaa aaaggctggc tatcatatca tcacagacgg
     6361 cgagttccgc agagccacat ggcatcttga ctttatgtgg ggcttcaacg gagttggtca
     6421 cagcaagaca cagacaggtc tgccattcca tggcgaagcc gcaatgatag acgatacata
     6481 ccttacaggc aaggtatcag ttggcgacca cccatttgtt gagcatttta aattcgttaa
     6541 ggctctcgag gacgagaaca cagtcgcaaa gcagactatc cctgcacctg cacagtttct
     6601 tgagcagatg ataatgcctt tcgcactgga gaacacaaag aagtattata acgatacaga
     6661 agagcttgtt caggatatcg caaagggcta cagaaaagtc atcgctgacc tttacgcatc
     6721 tggctgccgc aacattcagc ttgacgattg ctcgtggggc atgatagttg accctaatgc
     6781 aaaggctatt tttggagttg acgacgcagg acttgaggat ataaaaagac ttctgctgag
     6841 aataaacaac cttgctatcg agggcaagcc tgaggatctt gttatcacaa cacacgtttg
     6901 ccgtggcaac ttccactcaa catatgcaag cagtggcgca tatgacagtg tagctgagac
     6961 gcttttcgca ggcgagaacg taagcgcata ttatcttgaa tttgacgatg agcgttcagg
     7021 tggctttgaa cctttgaaag cagtaagcgg cgacaagaag gtcgttctcg gacttatcac
     7081 atcaaagaag cctgagcttg aggacaagca ggctgttatc gaccgtatcc acgaggctgc
     7141 aaaatacatc tcacttgaca gactttgcct tagcccacag tgcggttttg cgtcctgcga
     7201 gataggcaac aagctcacag aggaggaaca gtgggcaaag cttgcacttg ttaaggaaat
     7261 cgcagaggaa gtttggggat aaccaacaga tttaacagat catacgagtg cagagaaagg
     7321 ataaataaaa acaggtagat aactcaattg aggtatctac ctgttctgtt catagttcac
     7381 tatataggtc attcatatta gttctttgaa aatgtctata tagtatctat caaattgatg
     7441 tgagtcattt ttttagctta aatatgttgc cacacgcaac atatttttat agcctatgta
     7501 cccaatcaat agccgccacc ggctgatagt gaccttccca tgtggcagtg gtttcgtcgc
     7561 acaatgcaag tccatgtcca ccgtattcat aaatgtggct ctcaaatgga atgtgatact
     7621 ttgaaagact gcacatatac caaagtgtgt tctcaaccct tacacagcca tcatcagcac
     7681 agtgccagat aaatgtagga ggagtgttct ccgatactct cttctccatt gaaacaagct
     7741 ctctgagctc cgatgatttt tcctcgccaa taatattgag tattgagcct tcatgagtgt
     7801 attcagggtc ggatgtgata acaggatagc ccaagataga gccgtttacc ttgatgtctt
     7861 caggattgca gccaagaggc tttgtgagca gctcgctgtt ccagaagttt gccattgttg
     7921 ccgcaaggtg tccgcctgct gaaaaaccgc atactattat cttgtcagga tcaatgtcaa
     7981 atttctccgc attatctcta acatatttga cagctgcccc acattccaaa gcgtcagtag
     8041 gaaatttttt tttgacgcat gagtatctta gcacaaatgc acatatgcct gcacccaaaa
     8101 atctgaatgc aacaggttca gcttcacgct cagaacacca atcatatcct ccgcccgggc
     8161 agattataat tgccttgtgc ttctttctgc ctatttcctc agtaagtgtg ttgtaatagc
     8221 aatcaagcac aggtgcgcag ccttcgcttg aaacgccagc cttttcataa tccactttta
     8281 tttctactgt ttctttaagc attttatttc ctccgaaaaa tttattctgt agccgcctgc
     8341 tggtctgtct gagaggacga gtcagcaggt gctgcatttt ctgtcatatt cacgcagacg
     8401 atataaccgt caacgttgtt gcctgagata gtgttaggac gctcattgtt gctgtcgtca
     8461 agtggtacag gcacgtttgt ggtgctgccc ttgtctgcgg caacgtagta aactgaatac
     8521 tcagtgtcgt caaaagccgt gaatgtaagc ggcttttcaa atgtataatc cttgacaaaa
     8581 tctatgtatt cttcaaggca aagcttgttt gctgtcatta tctcagcgtg tggtataccc
     8641 acatatctga aatgataagc catagggctt acacctgtga tgtcagcttt gtcctctggg
     8701 tatctgagga taagtccgta tttccagcag ttttcagata tccaactata atctccctct
     8761 cctgtgaact caggatatgc gcctgtgctt gcatcataaa gatgcaggtc aaaggcaagt
     8821 ccagtgtcat gctcgtagca ggcagagctg ctgtcttcat tatcttccgc aggcatagct
     8881 gtgtttatca taagtgttga tatcttcttg tcagcataaa aatcattgcc cattttgcta
     8941 aaggcttcaa aggtctcttt tttagccgtc agttgagtgc tgctggtgga cattatttga
     9001 cttccgttct tgtcaaacaa ataggaataa actgtgtcct tgttattcac tgtaccggtg
     9061 aaggagtggt cgccattaac gagaataaga tctccattta ccatatcagt tttgtttttt
     9121 gcggtaacat acatataatt gcccgtaagc ttttttacag cttttttact gctgctgtca
     9181 gccttgaccg cagaggagtt tgacgagctg tcggaaattt tcttcgggct gtcagctttt
     9241 gaactgcacg atgtagatat tcccagaatg atcaaaagca gtatggcaag agccgctgca
     9301 atattcttgt atttataacg ttttactttt ggcacttaac atcaccttcc tgtataacat
     9361 acacctaaat tataacacat ttttcgtaat aagtgaacca tatgccactg tagtgtcggc
     9421 acttttgtta tatcagacaa atgttttgcg ggcgttttat gcaacaagca caaacaaaat
     9481 aagggctgta ccgcaaaaca gtgcagccct tagcgataaa tattttgtta gtttcttaca
     9541 tagtcaacaa cagactttat gaatacacgt ccctcaggtg acttggaaag cttatcaaaa
     9601 tcgcatgagc aaactaccac cttgccgccg tccttttcat actcgtagag gagtgaaaga
     9661 tcgtggttgc ggtcaaagtt gtcgatagtt ctcacgataa catttttctc gcccttaaag
     9721 tcgtcaagaa taaccgagcg tgagttctga actatctccc accactgtgg cgttgagtat
     9781 ttttcccctg caaagcctgc aagagcaggg tgttcggtgt cgataagaag tcccattgtg
     9841 ccaacagggt caggtttttt catcatctgt gagatgatac agaacatatg atagcaccag
     9901 aagtcctgac agtagaagcc ctcaatagaa ttttcaagcc ttgaaaggtc agggacgata
     9961 agcactgtct tgccctgttt cagcaggctc tcagcctcag cgttcacctc gtcgaaaata
    10021 tacgcgccgc ttatatctgt cttttcaact gtcggtatca cccaaaggtc atagtgattg
    10081 cgtatatcag taccatcaat ttcaagtgca agcacagcct ttgtgttctt tgttacctgt
    10141 gggagagtaa actctacatc gcaaatgtca gtgtagttgc cttctgcaac aaaattcttt
    10201 tcgaccttgc caatgacatc accattttca agggtgagag tgcaaataag cttgccgtct
    10261 ttaaggtcag gtcggtaatt gcaaagctct gtgtgcgcct tgaaactgct gacagcttca
    10321 agaacatagc tgtcaaaacg cgccattaca acagcatcat tgcagaactc tctccactcg
    10381 ctgtctgtga taagtccctt gctgtccatg aatgcgtcaa gtacacccac aagggcagta
    10441 ccctgaccgc tgaaatcctg aatatcaagt atctggaaac caccgagaag tcttgagcgg
    10501 aaaacagcct ccatttcttc cttatagcac tgcacagcaa gcttgcctga gcatttgaaa
    10561 taatcctcag ccagcggaag cagacccttt tcatcaagtc tttctctgaa tacctcaaag
    10621 tttctggctt taagggagcc tgtgtatttc tctatctcct tgaagttcgg ataagtttca
    10681 tactgaccta tttcgtgagt aacgataggc acttcaggga taaaatctgc gtcagcttcg
    10741 cttgccttga cagttttcat ggtcgtgccg tactgtatct gaacagtgcc gtcctctgaa
    10801 atttccgtgg agcttgcctg cttcttaggt cttataatgc tgtcataatc gtgatttgcc
    10861 gcaggcttgt cagtctgaat atgaccaagt ggagcgtcac acatagcgta tgagcctctt
    10921 ataagcctgt cgtttgcaag tcttacgcca acaaagaaat cgtcattttc tataacgtta
    10981 gggaaccact ggaaattgtt tgagccttgt gtgtaaaggt gtctgttatc aaatttcttg
    11041 tagccgccta tgatatcgtt gagtatcttc tttgagcccc aaagctcgtt gcccattgac
    11101 atcatgcagt aggaagggtg atcgccgaag aatttcagca tattgaagcc ctcttcaacg
    11161 aggaaatcct gctcctcctg attgtggttt tcctcgccct cttctgtgat agttccccag
    11221 aaaggaagct gtggctccat ataaattcca aacatatccg cagcgataaa agcagcctca
    11281 gggggacagc aggtgtgata acgataatgg ttcatgccgt atgactttga tattttcatt
    11341 accctcagcc attcctcaag gtcggtaggt gcaaaagcgg tcttagggaa tatcatgccg
    11401 tcatgttttc ctctgagaaa ggttttttct ccgtttatgg tgaactttcc accctcagtc
    11461 ctgaactcac gcataccgat gatatgctca ctcatgtcgc cgtcaatatc tattctcagc
    11521 ttgtagagat taggctcatg ctcactccaa agcaggcaat cgtcgcccat tttgtataaa
    11581 gcgtcaagct tgccgtctgt gtattcaaag ctctgcacag caggtgtatg gttcttttcg
    11641 ctgttgaagc tctcagcgct tatgacagct tttccgcctt ttccccctgt tatgtcagca
    11701 gtcactctta ctgtcttgct gtggatatcg caccaaagca taacattttc agcgtgagcg
    11761 tttttgtagc ctataagctc gatgcgtcct gtgattccgt tccagttggt ttgagtgtca
    11821 ggtgaagtca ggtgtccgcc cttggtcttg tagccaacat ttgaaacgca gatagtcatt
    11881 tcatgcacgc cagtaccgat atactcgttc aggtcataga tgtgcggagt gcaaaggctc
    11941 tcctgcctgc ctatcttttt gccgtctata taaagttcag ttattcttgt tctttcaaga
    12001 taaagtttaa gattttcaca ttgcagagca gagaagtcaa cgttttttct gaaccatgca
    12061 aagccctcaa atttatatgt gtctgtaaga aagcctgttt ctctcttagt attgtattcg
    12121 ccttttttag cgttggaagt agtgtttgga aggtttattg tatcatcaaa aacaaggtca
    12181 gtacccttac attccttgtc aagacacagc agccactcgc cgctgaggtc aagcttgttt
    12241 atcattaagc aacgatcctt tcaagttcaa attttcaata tccattataa catatggtat
    12301 tgcccatttc aaccgaaatc gtttgattta accaaaatgc tataaaattt tgttgttttt
    12361 aacaataaaa tatgatacaa taagattaaa agaaatctgc caactggagg tgcaggaaaa
    12421 atgcttgaaa atacagaaag aaagctgttg tggctgtggc tcgatgttgc gttcggacct
    12481 gcggacaagc gtttgtggga cgttatgtca tattatgaca gcgtcgaaga aatgtgcgat
    12541 gatctattgt cactgcgctg tggatatgtt actcagcagg aggctgaaaa gatagcctgc
    12601 tgtacgttct ttcaggcgaa acaggtggct gaatactgcg agaaaaaggg tatatatnnn
    12661 nnnnnnnata tatcctcacc cctgacgatc ctctttatcc tgaacggttg aaacagatag
    12721 aatgtccacc tgctgtgctt ttctgccgtg gtgatgtaag ctgtctgtct attgaaaaga
    12781 gcgttgccat tatcggcact agagaaccaa gcgattattc tgtagccgtg gcggaggagt
    12841 tttcaagctt tcttgcagag cgaggagtaa atatcataag cggatttgct atgggtatcg
    12901 acactgccgc acaccgcagt gcgatgcgtg caggtggaag cactgtagct gttatgggct
    12961 gcggactgga atatgattat cctcgtggca ggacgggctt taagcgtgag atagcaacac
    13021 atggggtagc catatcggaa tttttcccgc aggcaagacc tgttcctgag aattttcgta
    13081 taagaaaccg cattgtttcg gcactttcac attgcgttct tgtggttgaa gcaggaattc
    13141 gcagtggcac gctgaatact gtaaatcatg ctttatccca gaataaagac gtttttgtta
    13201 taccgcctca tgacattttc agcgagaaat acgcaggaca gtgtgcgctt cttcgtgacg
    13261 gagcggtaga agcttattct ccaaaagata tcctctattt tctcaataaa gccaaaatgt
    13321 agaactacac ttagttagtg tttaatatca tacctgtacc aaatcaacaa tatctacgtt
    13381 agttgtaggg gagcggctca atttctgttt cgcagaaatg cgacgtgccg atcattcgtt
    13441 ttttacactc tatctaaatg aaaaaggctg cgactttttg ccgcagcctt ttgtgatatc
    13501 tgaaattact ttgaaagtgg gctttctctg tagataatgt cctctctctt tggtccgttt
    13561 gaaaccattg tgatagggaa gccgatatgc ttttcaacga agtcgatata cttcttgcag
    13621 ttttcaggaa gatcttcata cttcttgatg cccctgatgt ctgacttcca gccgtcgaga
    13681 acttcaaaaa caggctttgc cttttcaagc tttcttgttg taggaaagtc tgttgttacc
    13741 tcaccatcta tctcatagcc tacgcataca ggtatcttgt caagatagcc aagaacgtca
    13801 actactgtga aagcaacatc tgttgcgccc tggatacggc agccatactt agttgcaacg
    13861 cagtcgaacc aacccattct tctaggtctg cctgttgttg caccaaactc tccaccgtcg
    13921 ccgcctctct ttctcagctc gtcagcctca tcgccgaata tctcagaaat gaatgcacct
    13981 gcaccaactg cggaagaata agccttaaca acagttacga tagtctttat ctcataagga
    14041 ggtatacctg nnnnnnnnnn tgcaccgata gcaccgtatg ctgccagtgt agatgaagat
    14101 gttaccattg gatagatacc gtggtcaggg tctttcagtg aaccaagctg accctcgaga
    14161 agaacgttct tgccttcttt gatagcgttc caaaggaatg cagatacgtc gcaaacgtat
    14221 ggtgctacca tttccttata ctccataaga gtattgaaaa gctcatcagc gtcgatagga
    14281 ggcttgtgat agagatgttc aagaagaatg ttctttgttt caagaactct ctttatcttt
    14341 accttgagag cctcttcgtc aaacagctcc tgtacctgga aacctatctt tgcatattta
    14401 tcagaataga aaggtgcgat acccgatttt gttgagccga agcttgcctt tccaagtctt
    14461 tcctcttcgt actggtcaaa cagaacgtga tatggcatta ccatctgcgc tctgtcggaa
    14521 ataagtatct taggcattgg aacgccttcg gaagttacct ttttaagctc attgaaaaat
    14581 acagggatat taagtgcaac accgttgccg ataatatttg ttgtgtgatc atagaatacg
    14641 ccggaaggca aagtgtgcaa agcgaacttt ccgtagtgat ttacaatagt atgtcctgcg
    14701 ttagcaccgc cctgaaatct tactactata tcagactttt cagcaagcat atcggtaagc
    14761 ttgcccttac cctcgtcgcc ccagtttgcg ccgacaatcg ctctaaccat ttaattttat
    14821 cctccttgcc ataaaacagc aaatttagta gcaga
//
LOCUS       DAWEMR010000052        14172 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1777_length_14172_cov_10.167599,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000052 DAWEMR010000000
VERSION     DAWEMR010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14172)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14172)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14172
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1777_length_14172_cov_10.167599"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2573..2582
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4143..4152
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12207..12216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12604..12613
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3809 a         3094 c         2634 g         4595 t
ORIGIN      
        1 cacaacatag aatatttttg ttttttgcgc tgtctacttg acagggggcg ggtcataaca
       61 taccgtccat aaaattttat cttcttgaag cctttttctt tgtgctgcct gttttagaac
      121 ctgaatgtga cttctttgcc tgatgttttt tccaatgtac ccacagtgta ggtgcgatac
      181 agttagaaga ataaacacct gatatcatac cgatgataag tgggaaagca aagctcagga
      241 tagatgttac tccacagatt atgcagacga tacaaactgt agccatagca agaactgttg
      301 taacagttgt atggatagaa cgtcccattg tctgtgtaat agagaggttt acaagatcct
      361 caagctccat tttattgcca aggagatttt cattctctct gattctgtcg taaatgatga
      421 tagttgcgtt gatagaatat ccaaggatag tcagcaaaac agccatgaag tttgcgttga
      481 tgtcaaatct gcatattaca aagcagccat aaaccatgca catatcgtgt gcaagggcaa
      541 ctactgagaa cacacctgct gagataccgc ctatcttctt aaatctgaaa ccgatataga
      601 tgatagtgat gaccattgcc agtgcacaag cagcaaggca cttgaagaag aagttcatac
      661 cacttgaagc tgcaacgtcg tttgactcgt aaacttctat gttgttatca ggaaagtcct
      721 tttcaagggc tgttttcagg gtgttctgct tatcatcgct tatacccttt gttgatgaga
      781 attttatttc tactgtcttt cctgcgtttc ctgtgttttc gccaagagtt actgtacatt
      841 tatctccgat actgtcagaa acggtatttg aaacgtcaga agtctttata tcgccctcat
      901 atgtataagt caaaactgta ccgcctttga actcgatagc cacgttaaga cctataacga
      961 atgtaagaat gataaaaatt gcgataagtg cgcatgatat ggaataaaat aacttgctgt
     1021 gacccacaac attcagggtc tttctttctt tactcattta tttcagcacc tccgtaaagc
     1081 ttgcgtttct taaagcactt gaactttgag agggacgaaa gcataagtct tgaacagaag
     1141 ataccgaata caaagttcag gaggatacct acaaggagag tatatccaag tgaatagata
     1201 gtacctgctg tgcttgcgcc gaatactgag aatacagggc tgagaagtgt tccgaaaacg
     1261 ctgtctgtag gtccgaaggc acccataagg attattgcaa cgaatactac tgtgatgttt
     1321 ccgtcgaata ctgcgctcca tgctctctta tagccaagct cgatagctcc gttaagggtc
     1381 ttgccgttgt taagctcttc ctttattctc tcggctgtga taacgttggc gtcaacgccc
     1441 ataccgatag aaaggataat acctgcgata ccagggatag tcagcgtaaa gctgtcgata
     1501 tttccaaaga agcctgtgat acaagcgata gaacctatta cctgacctat aagtgcgata
     1561 gacgcaacaa agcccggcag cttatagata atgatgatat atgcagcgat aacgataaat
     1621 gcgataattc ctgcaagcac cattgcgttc tttgcaccct cgccaagtgt aggtgaaatg
     1681 atgttcgtgc ttgttgtttt aaggtcgaat gggatagaac ctgcatttat cttatctgaa
     1741 agagcctttg cggaatcata tgtgaatttg ccctcgataa cgcactcacc gtcagtgata
     1801 gctgaattta cgcttggggc agaaatgcac ttatcgtcca tccagataga gataaagcct
     1861 tttccggcaa gctcagttgt agcgtcagca aaagcttttt taccgctgtt attgagtgtc
     1921 aggcttacta catacttgat atttcctgta gtttcgtcct gagtttgtct tacctcagcc
     1981 tttttaacgt catttcctgt aagcacctgc tcgccttttt cttctttacc ctttctgaat
     2041 acaagctttg cagtttcgcc aagctcagaa acagcagcct cagggtcaaa gctttcttcg
     2101 ttagcctgcc atgggaatga aaccattacc ttatttttct tatagtctac atagacctcg
     2161 ctgtctgtga tattaaggct tacaagtctt tgctcgataa cagcctttgc agcgtccatt
     2221 tcttcctttg tggcgtcgta attatcggca ggttcaaacg tagcatttac gccaccctga
     2281 atatcgatac cccagcggat atcttcaacg cccttgatat agcttgtttt tatatctccg
     2341 tactgagtat gtattcctgc ataagtgaga tatccaaatg caattataca caaaagcacg
     2401 ataaaaaaca cgggtttttt aacttttcgc acctttaatc ctcctgataa atttattaag
     2461 cccagaggct tttctgccgt catggcacaa tacgcccata acgaacacat tcaccttata
     2521 attataaaac gtttgataca aaatgtcaat agtttttatg aaattcgcac tgnnnnnnnn
     2581 nnatctatga aataagttct ctcaaaacct gtttcaaaag cctgttttaa aggggctttg
     2641 tcttgacaaa atctcctttt agtgatatta ttaaaaggat aaatattgcg acgtaaggaa
     2701 atttttttgc gtcagctaaa aggagtatgt aaaatgatag atattaagct tatccgtaca
     2761 aatcctgagc ttgtcaagga aaacatcaaa aagaagtttc aggacgaaaa gctcgttctt
     2821 gttgacgagg tagctcagtt tgacgaggaa tacagagcag ccaagacaaa ggctgacgag
     2881 ctgagaaata aaagaaaggt aatgagcaag cagataggcg gtcttatggc taagggtcag
     2941 aaggacgaag ctgagaaagt aaaggctgag gtttccgcta tcggcactga gcttgacgct
     3001 atggctgaaa aagaaaaaga ccttgaagct aagatacgtg agagaatgct cgttatccct
     3061 aacataatcg acccaagcgt tccaataggc aaggacgaca gcgagaacgt agagattgaa
     3121 aaattcggag agcctgttgt tcctgattat gagatcccat atcacgttga cattatggaa
     3181 agctttaacg gcattgatct tgacagtgca agaagaacat caggaaacgg cttctattat
     3241 ctgatgggcg acattgcaag acttcattct gcaattcttt catatgccag agatttcatg
     3301 atagacagag gtttcacata tttcattcct ccttacatga tacgttcaga cgttgttaca
     3361 ggcgttatga gcttttctga aatggaaggc atgatgtaca agatagaggg tgaagatctt
     3421 tatctcatcg gtacttctga gcattcaatg ataggcagat ttatcgacac tatcattcca
     3481 gagggtgaac ttccaaaaac tctcacaagc tattcaccat gtttcagaaa agaagttggc
     3541 gcacatggca tcgaagagag aggcgtttac agaatacacc agtttgaaaa gcaggaaatg
     3601 atagttgtct gcaagcctga agatagcatg gcatggtatg acaagctgtg gcagaattct
     3661 gttgatttct tccgttcact tgatatccct gtaagaacac ttgagtgttg ttcaggcgac
     3721 cttgcagacc tcaaggtaaa gagctgtgac gttgaggcat ggagcccaag acagaagaaa
     3781 tattttgaag ttggttcatg ctcaaatctt ggcgacgcac aggcaagacg tcttggcata
     3841 agaataaagg cagaggacgg cacaaagtat tttgctcaca ctcttaacaa cacagttgtt
     3901 gcacctccaa gaatgcttat cgcattcctc gagaacaatc tcaatgctga cggaacagtt
     3961 aatattcctg agccactgag aatgtacatg ggcggcaagg acaagataac aaagtaattt
     4021 tgatatgaaa aaaggtgaac ggtcatgtgc cgctcacctt ttttttgttg tctgaaaatc
     4081 atatagatac aaaaaacgga cggtttttag ccgtccgtta atttttttag tcaatgtagg
     4141 ggnnnnnnnn nngggcgaac agtgttcgcc cgtttatttt gtctttactt gatttttata
     4201 ttattcttcc gtgttctcag aatcctcatt ctcaacgctt tcggtattat cttcaatgac
     4261 ctcagcattt tcctcagcct gagcctctgc tatctcctcg gcagtagcct tttctacctc
     4321 tgctgtttca gcatcatcgt catgctcagt tcttgtgaat gaaaccactc tgccgtcctc
     4381 gccgactctc ataagtctta cgccacggga gtatctgctc atcatgctta tatcgcaggc
     4441 tcttattctg ataacaatgc cgtctgtgga tatcatgatg atatcatctt catcatcaac
     4501 gacctttatt ccgcatactc cgccactctt atagttcata agaccgtagc cgcctctgtt
     4561 ctgtacctta taagactcca gcggaacacg tctgccaaga ccattttcag caacagtgag
     4621 aacgcttgca ccctctctta ctcttgccat tgatacaaca tagtcgctgc ccctgagttt
     4681 tatagccttt acgccgtgtg caactcttga catttctctt atatctgttt ctcttatcct
     4741 gatagcataa ccattatgcg ttgcaacgat aacctcattg tcgccaaagg tcattcttac
     4801 tcctgctatc tcatcgccct cgtcaagacc gattgcaata agaccattct ttcttacatt
     4861 tctgtaggaa gtcagcggag ttctctttat cttgccgttc tttgttacca tgacgatata
     4921 cttgccctcg tcaaagtcgg cagtttttat cattgcggct atcttctcgc cctcgcccag
     4981 ttcaagaaga tttactatat ttgtgcctct tgaagccttt gagccttccg gaagctcata
     5041 gcatttgagc ttatacatta tgcccttatt ggagataaag agtatattgt catgtgaaga
     5101 acaggtctgg agttcgtcga tataatcgtc ctcacgctgt ttcatacctg ttacgccacg
     5161 tccgccacgc ttctgagcct tatactctga aacaggcatt ctctttatat atcctgcatt
     5221 tgtataggta acaacgcttt cctcaacagg gataagatct tcgatatcaa cttcgccgct
     5281 gacattttct atctgagttc ttctctcgtc gccgaactta tcctgaatag cctcgacctc
     5341 ttcaatgatt atatcaagta ttctctgatg atttgcaaga atatcttcaa ggtcagctat
     5401 cttgacttcg atctcagcca actcatcgat tatcttttgt ctttccatac cggtcagacg
     5461 gccgagagtc atatttgcga tatggtcagc ctgaatatct gtcagaccaa aacgctgatt
     5521 aagtctttgc ttagcctctg ggatattctt agaagttttc attatctcga taacttcgtc
     5581 gatattgtca agtgcgataa caagaccctt taagatatgc tctctttcct tagccttgtt
     5641 aaggtcgaac ttagttcttc ttgtgataac atcgacctga aagtctatat actgctcaag
     5701 catttccttg agggtcagca ccttaggaat accatttaca agagcaagca ttataacgcc
     5761 cacagtatcc tgaagctgtg tatagctgaa cagtttgtta agaactacct gcggattagc
     5821 gtcctttttc agctcgataa cgatcctcat tccgtccttg ccggattcat cgtttatata
     5881 atgtattccg tcaatacgct tttccttaac aaggtcagcg atagactcaa caagtcttgc
     5941 cttgtttacc atgtaaggaa tctcagtaac gataatgcag tttctgccct ttatctcctc
     6001 aatagaggtt tttgaacgca gtattatctt tcctctacct gtgccgtaag ccgctctgat
     6061 accggacctg cccatgatga taccgcctgt agggaagtca ggacccttta tgctctgcat
     6121 aagctcatca agtgtgcagt cagggttttt catgactgtt ttcatgccct ctataacttc
     6181 atggaggtta tgaggaggga tattcgttgc catgccgaca gcgataccta ctgagccgtt
     6241 acagagaaga tttggaaatc ttgacggcag aacaacaggc tctttcagtc tgtcatcata
     6301 gtttgactga aaatcaacag ttgttttgtt tatatctgtc agcatattta ctgacatctt
     6361 agccatttta gcctcagtat aacggtaggc agccggaggg tcgccgtcaa cggaaccgaa
     6421 gtttccgtga ccgtcaacaa gaggatatct cattgagaac ttctgagcaa gtcttacaag
     6481 tgcgtcataa actgatgcgt caccgtgtgg gtgatattta ccaagcacag atccgaccgt
     6541 atctgcacat tttctgtaag cttgatcagg tgtaagacca ttttcataca tggtatataa
     6601 aattcttctg tgaacaggtt taagtccgtc ccttacatca ggtaaggctc tggatacaat
     6661 taccgccatt gaatattgaa tgaaagattc tttcattatc gcttcaacat cagtgtcaat
     6721 aacgttataa tcgctcactt tatccactcc tttttaacct ttcggtttac tttgaaaaga
     6781 acatatcctg ttcttattta ttatctatca tttcataatc gctatcaagc agctctgaaa
     6841 aagtttttct cagtgtttcc tgctgctgct cgttcataac tctttttgca agcacataaa
     6901 tagtttcctt tttgagtatt ataataaaca tttcgtcatt ttccaaaacg ttcagtgaga
     6961 tatcctcaaa accaactatc ctcggtggaa caggggtaaa gtcctcctcc tgcttttcct
     7021 cctctgtcgg cagcgtttct attttgaagc tgtcatcata gagggtgaaa acatacttgt
     7081 cgtcctcaag aggtttaagt gcctgcataa gatattttct tattcttctg gtgttatacc
     7141 atgtgagaac gattattgca aggcatatga acgccagcac ccagttaaga tatcccgaag
     7201 ggtttctcac tatggacact ataaaaagca ccgcaacaac gccgaaagcc gctgttatct
     7261 tccagttatg cgcataaacg aattttctct gaaaggctaa aaaagctctt tcctcttcct
     7321 cgtttttcac atcatactca aagctcaaaa caggctcttt ttctatctgt gcttcttcca
     7381 cctcatcagc attatcaaca gctttctcag ccgtttcaac agcttcctct gtttttacct
     7441 cttcaagctc tgtttcctga tttttatttt cgttgttatc caaagcagta tcctttcttt
     7501 aaacgtcaag atcctttaca tactctgcat ttttctctat aaattctttt ctcggtgcga
     7561 ccttatcgcc cataagaata gtgaatatct cgtccgcctt aacagcgtcc tccattgtca
     7621 cctttatcat agttcttctt tcaggatcca tggttgtttc ccaaagctgt tcagggtcca
     7681 tctcaccaag acctttatat ctctgaacgt ttactttcag attgttttca cccgaaagct
     7741 cagaaatata ctgatctctc tctgcgtctg agaaagcata tctgacctgc ttgccccttg
     7801 caaccttgaa aagcggcggc tgagcaagat agatatgacc ctcctcaata agaggacgca
     7861 taaatctaaa gaagaatgtg agcagaagtg ttctgatatg agaaccgtct acatcggcat
     7921 cggccatgat gataactttg ccgtaacgaa gctttgaaag gtcaaagtcc tcgccgattg
     7981 atgttcccaa tgctgtaaca acaggcataa gcttttcatt gccgtatacc ttatctatcc
     8041 tggctttttc tacgttgagc atttttcccc acaaaggaag tatagcctga tattttctat
     8101 ctctgccttc ctttgcagag ccacccgcag aatctccctc gacgatatag atctcggtga
     8161 gtgaagcatc tctctctgag caatctgcaa gcttgccagg caatgaagat gattcaagag
     8221 cagattttct tcttgtaagc tctcttgctt tctttgcggc ttctcttgcc ttttgtgcct
     8281 gaacggactt atcaaatata gcctttgcca cagtaggatt ttcctcgaag tagttcataa
     8341 gcttttcggt aactatcttg tcaacgaaag gtcttacctc aggattgcca agtctgccct
     8401 ttgtctgtcc ctcaaactca cagtttgtga gcttaacgga tactatagcc gtcagacctt
     8461 ctcttacatc atcgccaaga agcttatcgt cgtctttaag atagccgaac ttcttaccgt
     8521 agttatttat tacctttgta agagcatttt tgaagcctgt ttcatgagta ccgccgtcag
     8581 gagtatggat attattagca aatgaaagta taaggtcatt ataagtatcg ttatactgca
     8641 aagctatctc ggcaaaagta tcaccctcca tgccgttgac atagataact tcctcagaaa
     8701 gcggagtaac gcctcttgtt ttgtggatat gctccacaaa ctgtcttata ccgccctcat
     8761 aatggagagt ttcgcccaca ggctctgatt catttctctt atctgtaaag attatcttta
     8821 taccagcatt aagaaatgcc tgttctctaa gtcttttaag cagtgtttca tagtcaaaca
     8881 ctgttgtttc ctgaaagatc tcagggtcag gcttaaagag aacggaagtt cctgttttat
     8941 cagtatcgcc gattattttc agcggctctg aataattacc tctgtcaaac ctctgaaaat
     9001 gaatatgttt tccgtcataa actgtaagct caagatattc cgagagggcg tttactacag
     9061 acgcaccaac gccgtggagt ccgcctgcta ccttatatcc gccgccgccg aactttccgc
     9121 ctgcgtggag aatggtataa actaccgttg cagcagatat tttctccttt gggtgtatac
     9181 ctgtagggat accacgtccg ttatcggtaa ccctgataat gtccccggga agtatctcta
     9241 cttctatctc agtgcaatat cctgccaatg cctcatcgat agcgttgtca actatctcat
     9301 agacaaggtg gtgaagtccc tttggacctg ttgatccgat atacatacca ggtctttttc
     9361 ttaccgcttc aagaccctcc agcacctgta tctgattttc attataatca ttttgcttat
     9421 ccaccatgga tatttcctcg gtgttctgga tctcgatctc gttgttattt tccaaaaaat
     9481 aaccccctca tcacttggtt ttttatctta tcatcttatt tctttgattc atcattcagg
     9541 cattcttttg cttcttttca aaagtgtggc acagctcatc tgacttatat acacagtttc
     9601 cgtaaagtct ttatcaaggc acaccacaaa gcttttaggc atttcatatg tgcagttcac
     9661 aacacgtctg ttttttgccg caaaattaag aaattccttt gtgttggcag aaacagaggt
     9721 gttctctata tcgaagatcc ctatgatatc tttagtattg actaaatagt tatttcctaa
     9781 atgcagaaac atcacttatt ctcctgaact atcattccgt tttctatatt gaatatcttt
     9841 ccatggcttt tctcgtcaaa ggggatattc ctgtcacagc aggttataaa gacctgcata
     9901 tcgtgtattt tcgagataac gaacttctgc cttgataggt caagctcgct caaaacatca
     9961 tcgagaagta tacacgggct gtcatctatc tcctcggcaa gtatataagc ctgcgaaagc
    10021 ttcaaaataa gagcaagaga gcggtgctgt ccctgagaag cgaattgtct aactgccagt
    10081 ccgtttattt ttccagcaag atcgtctctg tgaacgcctt tctgtgtaaa tcgacatcaa
    10141 gattattttg cagacagctt agatactctt cagccatttc tcctgcataa tcttttctgc
    10201 cctccagatt tgaatacaca gtggaataat atccaagctc caacctttct ctgccctctg
    10261 atatatcctc ataaagcttt tttgcgaaca tatcaagctt tttggtataa ttatatctca
    10321 aaagcgagat ataagcccca agctgagcaa gctggatatc ccacacttca agctcttctc
    10381 tttttgatct gccgtaacag atatttttta acaatatatt tctctgctct ataagatcat
    10441 catattttga gataacagag ctgtaggaat tttttatctg tgacactgaa aggtcaagaa
    10501 actgccgtct gttttcagga gagccttttg aaagctccag atcctcagga gtaaagataa
    10561 cacaatcaag gcttccgaaa agctttgagg gagttttcag ctttacgccg ttcaaggtga
    10621 cgtttttttc tctcacatta ggtcttgcca ttgcaaaatg tatatcctga ctgcgaaagc
    10681 tgtttttaaa tgaaatgtcg atattcatga cctgtccgtt tatatctatc atttctctgt
    10741 cttttgtgtt gcgaaaagac ctcactccgc tgcaaagcca tatagcttct ataagatttg
    10801 ttttgccctg agcattcttt ccgcagaaaa tattcatttt ctcatgagga tatatctcga
    10861 cttttttgag atttttaaat ccgtctgcgg aaattttggt tatcaccatt ttattttacc
    10921 actatctctg tagctatctc gtctatctgc accttatccc cggggcggat cttctttccc
    10981 ctcattgtgc agacctcacc atttactttg attctctcct cgaggattat ctccttgcca
    11041 attccgcctg tttccacaac gcctgcatat ttaagcagag aatcgagctt tataaactct
    11101 gattttatat ttacatctat ctgttgatat ttcatattct tattcctttg ctagtcttac
    11161 agggagaacg aggtaagtat aagcgtcgcc ctcacacgga actatcttca taggcagcag
    11221 acttccgctc atctgcaatt tgaccttatc ttcctttatg accttcagag ggtcaagaag
    11281 atatttgcac ttgaagccta tctcgataac aggacctgct atatcagcct ttatctcgtc
    11341 tgttatcttt ccaacgcttg ttgagcagtt tatttttata gaattatttt caaaatagca
    11401 tcttgcaggt gacggtgttc tgtcacttat aagtatcaaa cttctttcaa gagcggcaat
    11461 aagctctttc ctgtcaacaa tgacctcagt tgtgcaagta gttggtatag ctgacttata
    11521 agggtggaac tcgccctcga taagtcttga ataaacaaga tagccgttta attcaaacac
    11581 aacatgattt tttgagagat aaacggaggc tgtttcttcg tcattatctc ccagaagtgc
    11641 tgctatctca gagagagttt ttgcaggcac gacgaattta agacttccgt catactttat
    11701 aggctcatat ctcacagcaa gtctatatcc gtcaagggca acaagagtaa gagtaccgtt
    11761 ttcgatctca aaaagctcgc ccttaagaac aggcttaagg tctgtaacag ccacggcaaa
    11821 ctttgtctga tttatcatat ccttaagaac aggctgagag atagttatct tgttttcttc
    11881 ctctttctta ggaagtgcag gatattcctc tgctgatgtt gcagggatat tgaatgtggt
    11941 aactcctcct gagatcgtta cctgaaaatt ttcagaaacc tctatcagga tctcttcaga
    12001 aggcattttc cttatcattt cagcaaaaag tcttgcatta agtataaatt ctcccgaatc
    12061 gtcgctcttt actggtataa cagtttttat acctatctca aggtcatagc cggtaagctc
    12121 cagcattgag ttttcaagct tgaattttat tccctcaaga gatttaagtg cagcacgttc
    12181 tgacactgct tttgaaacat ttgttannnn nnnnnnaatt ttcatagcaa tttcctttct
    12241 aaaaaaatta catttatctg acttttgttt tttcaacata tgaatttctc aaatttatat
    12301 tttttgctct gataatatat aaataaatca atattaaatt agtattagta gtagagtgtg
    12361 ttgaaatggt tgaaaagtca ttcaaagcct tttgcagact tgttttgctt ttcaagttta
    12421 ttttaacatc acttcaactg tgttttcaaa aacttttttc gtgtttgaaa agttgaaaaa
    12481 atgtttgttg aatgtttatt attatcgcaa aaaatgttga aacctgaaaa tcggtgtttt
    12541 attttataac accattgttt aaacttcatt gtgcaaagtt ttgaaaagca ttccgcaaag
    12601 cttnnnnnnn nnngtccgga tattcaacaa aattgttgaa aactatgttg aaaatgttga
    12661 aatagtccgt ttaattgtcc ttgaggtttt tgattatgtc gtcaacggta tcttttagct
    12721 gtttgttctc tttaagattt ttaacaacgt cctgatagtg tatggtcata gttgaatggt
    12781 ttttgttaag ctcatttcct atctgtataa aggtcatgcc ctttacgtcc ttgaagagat
    12841 agatcgctat ttttcttgca agggatacgt ttgcacgtct gtttgttgat tttatgtcgt
    12901 cagcagtaac gtcaaatgcc gccgctacct cctgaagtat cctgtcaacc gttatctcag
    12961 cagggtgatt ttcaatgatt atttccttga ttatcctctg agccatagat atggacgggc
    13021 tttcatttgt ataaatggta agagccttta ttttatttac agttccctca agctgacgta
    13081 tgttcgtctt tatcttctct gctattatcc ttacgacatt gtcagaaagc tgtaagtcca
    13141 gcatatcagc ctttttcttt acgatagcca ttcttgtttc aaaatcaggc ggtgtaacgt
    13201 ctacctgaac gcctagtgaa aatctggtaa gtatcctgtt ttgaagcttc gatatctttg
    13261 acggtgcaat atccgatgtg agaactatct gcttgcccgc attgtaaaga tcgttgaagg
    13321 tatggaaaaa ctcctcctgc atacgctcct tgcctgcaat ggtctgtata tcgtctacca
    13381 taagatagtc ggcattcctg tatttatcat gaaatttatt agtttccttt gactcgatag
    13441 ctttaacgag ctggtttata aaggattcac ctgtggtgta aataattttt agctcagggt
    13501 gatttttctt tacttcatgc tcgatagctt tcataaggtg agtttttcca agtcccgaat
    13561 caccgtatat gatgagaggg ttgaatatag tatttgtgtt agacggcgtt ttctgatcct
    13621 cgtcatactt atgtgcgaca gccgtgctga aagcataggc aagatcattc gatttacctt
    13681 tgataaagtt atcaaaggta agattgttgt tttcttcatc agaaaagtca gccaccttga
    13741 cttctatatc cttttcctca gtttcattaa cttcttcttt gtctttaaca agtattttta
    13801 tatcaacctc aaagcccaaa gtgttgagaa aagcttcttt taaaagattc tcataggcgt
    13861 tcatagtgat atctcttgaa aaatcagaat cagtgagcaa ataagccaca ttgttttcaa
    13921 gcttcatcgg ttcaagtatc tttatccatt tgttatatcc cggctcgctg atacttttgc
    13981 agtttcggca atatttcagg acctcctgaa atacgtcatt aaaggaatcc attgtttctc
    14041 cataacctcc tagaaaatag ctctgcctgt gcggtgtttc cgctgtccgc cgtgacccct
    14101 gtcaaacaga cggcaggtca gagtttaaaa aaaaatataa acaggcataa ttaatcaatt
    14161 ctatactatt at
//
LOCUS       DAWEMR010000053        14142 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1782_length_14142_cov_11.597288,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000053 DAWEMR010000000
VERSION     DAWEMR010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14142)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14142)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14142
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1782_length_14142_cov_11.597288"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5250..5259
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3830 a         3659 c         2678 g         3965 t
ORIGIN      
        1 tccttcgaca ttataccatt ttagcaaaga tctatcaaca cccaaaatct tattttaata
       61 ttaaaaagct gttaaagcaa cggtgcaaat atccttagca cgccgtcaat gagccgtcca
      121 aagaaaccac gctttgtgtc ctcaagcgtg cgctgtctgc acttgctctg agtgtccaga
      181 atgtccgcct taaaggtctt tattatagat gaatcaaaga atatggagct gcactcaaaa
      241 tgcaggaaaa gacttctata gtcaagattt accgaaccaa gcaccgcaag cttgtcatcg
      301 catatacagc cctttgcatg aacaaatccc ggcgtgtatt catatatctt tacccctgcc
      361 tcgataagct gacgataata gcttctcgaa agcctgaaaa ccaccttttt gtcagcgata
      421 ccgggcatta tgatacgcac gtcgacccca cgtttagccg cccttataaa agcgtcaaaa
      481 aggctgtcac caagcataag atacggtgtg tagaaccaca cataatcgct tgcggcatag
      541 agcatctcag aaaaaaacgt gttgctcaca ttttcgtcca ccatagggga atcataataa
      601 ggcaaaacat agccgtcgcc acccttgccc tctttttcaa agctctctcc cacaagttca
      661 tgaggtatca tgtcatttga aaaagcgttc cagaactcca taaacatata ggtgtagctt
      721 tttacgccct cgcctgttat cttaaagccg atatctttcc agtgaccata tttcgagcct
      781 atgtttatat actcgtccga aagatttatt ccgccgctgt atgcaactct gccgtccacc
      841 accattattt ttctgtggtc acggttgttg agctgtgatt ttataaaaag gaacggattg
      901 aaaggcacac attttatacc tttctcacca agcttcaaag cgtctgcaag agaatatgtt
      961 gcgatacttc caaggtcgtc atacattatc ctgacgtcaa cgccctgagc cgcctttttc
     1021 gccatgatat ccacaacagt gttaaggaac ttgccgtttt gcaaaataaa atactccgca
     1081 aaaatatatt tctcagcctt ttcaagctcc ttgcacatat cagcgaacat ttcctcgccc
     1141 acggaaaaat actctgcgct gtcaagcttc accataggaa atcccgttgc gtcgcttatc
     1201 cttttcaccg attgagcaag ccttttgtcc tcacgttcaa gctcccctat gcacttttcc
     1261 ccgtcagaaa gctcatagtc catgtgaagc cttgcctttg tgatattctt ttcaagcttt
     1321 ttcgcagtat tgttattgcc aaaaattata tacataactg caccaagcac gggaaaagta
     1381 agtatcacaa taagccaaag ggtcttgtag ctgctctcct cacgctttgt gttgatataa
     1441 agcacaaaca gaaatgacag cacagtaagc acgatgctga gaagccctgc cgccttgctg
     1501 agccatttaa gtatgagaag ataccacaaa aactgcatcg ccacaggaac tatcaccgcc
     1561 accactctgc ctgctatttt ctttacattg ttccacgtca actccgtttc gctcctttca
     1621 tttatcatct taggacaaca aaacactatc attatgcgtt gttaccatta ttataacaat
     1681 ttttttaaca taagtcaacg ccattgattt tattattaaa aagtgttata ttaaatatga
     1741 gatatgtcct gctgatagct ttgtctgtag gcgggcgaac agtgttcgcc cctacaaaaa
     1801 taatataatt gataacggag gtaacaaaat gtgtactgcg gcaacttata aaacgaacga
     1861 tttttacttt ggaagaactc ttgactatga aagctcctac ggcgaggaaa tagtcataat
     1921 gccaagaaac tttcccctta actttgttaa tatgggcagg atagctaccc attatgcggt
     1981 aataggcaca gctcatgttg ctcaggatta tcctcttttc tatgacgccg ccaacgaaaa
     2041 gggtcttgcc atggcaggtc ttaactttgt tggcaatgcc ttttaccgtg atgatatcga
     2101 cggcagggac aacgttgcgc agtttgagtt tatcccatgg atactctgtc agtgtgcaaa
     2161 cgtgacagaa gcaaggtcgc ttatagaaaa gataaacata accaaaactc agttcagccc
     2221 gcaaatgccg tcaggtcagc tccactggat aatagcagac aagaacgatt gtatcgtagt
     2281 ggagtctgta aaagagggca taaaggtcta taacgatccc gtgggagtgc tgacgaacaa
     2341 tccaaccttt gatatgcaga tgttcaggct caacgatttc cgtggacttt cacctaaaca
     2401 gcctgaaaac acattctctg acaagcttga ccttatttcc tacagccgtg gcatgggtgc
     2461 aatgggtctg ccaggtgact tatcctcgca gtcaagattt gtaagagccg catttgtgaa
     2521 aatgaactca cgctcaggcg cagacgagca ggacagtgtg agccagttct tccacatttt
     2581 aaactcagtt gaccaacaaa gaggctgctg cgaagtttcc gagggcaagt atgagataac
     2641 catttataca agctgctgca atactgacaa gggcatttac tattacacca cctacaacgg
     2701 acatcagata tccgctgttg atatgcacaa agaagacctt gactccgcca agcttattgc
     2761 ctaccctatg ataaccaacg agataataaa ctttcagaac tagcaaacgc tccgtacaaa
     2821 atctgtacgg agcgttttta ttactcaaat ctgaaatatt caaagtcaaa gttgcttccc
     2881 ggcaggaacg caaatgaaac cttctgtttg ccgctgatac ctgagatatc aaagctcttc
     2941 tggacataat tttcgctgtc atctgtcctg aactcgcaga gtatgcggct ttcgccctcg
     3001 ctgccgttca aaataagatg tatgctgttg agggcaagca gtgactttcc acttataacg
     3061 agcctgctcg gctgtttttc agaaaaatcg aactcgccga aatcaagcat aacattgttg
     3121 cctatctcgg tcacacactt ttcgcccttt gtgaacttgt caccataaat gttgtcagca
     3181 tcagcggcat tattctccgc aaattcacgg ctcacctttt caaagttgaa accgcccact
     3241 tggaagccgt catcagtaag gatacaaagg gtctcaatac ccttgagctt tcttgaaagc
     3301 ttgaaagttt caggcttgaa gatcatccac tcaggctctt tgtgataaca aaacgtgcca
     3361 agaagctttc cgccctcctc aggtattccg tcatagaagc ttattttaac agggtcaagg
     3421 gtgttcgccc atatctgcac cgtaacagtg tcagagcctg cactgccgaa gtcaagatta
     3481 tcatatgccg cccatgatgt taccttgccc attgcaaagc ctacaccctt cttcatgccg
     3541 cttgaaacgt tgtcgcttgc ccttgtgaaa agtccaccga taacgaactg atatgggtcg
     3601 gtaagtgcgt ttccaagacc ctcagcagtg aatttgagca tagaaatgat gtgataacgc
     3661 tcagtgccgt ttttgcacat tgcacgaagc cagaagcttc cgtctccctt tgctttcacc
     3721 ttaatgctgt ctgctgttac ctcgcactct gcaagattag atgttatgcc cttttcattt
     3781 gttaccctga actctatatc ctcagcatag tctgaatttt caggtagagc cttatatttc
     3841 actgttatct cagggttatc cttgtcaaga gtaaaagtat caatataaag ttctatcttt
     3901 ctcactggta tctcgtctgt tcttccgcac tcggtaggag caaagcccac attctccagc
     3961 gatgatgtgc cgctgtcata ttctgcctta acagcatcaa gggtaacaac acagttaggc
     4021 agaccctttg atgtaagcgt tacctttatc tcgccggtct tgtcagtcga accgataaca
     4081 gcaagcattt ttcctgagaa aagtctgcgt gatgtgcctt tatactggtc atagtcagtg
     4141 ctgtcgccgt tatcaagacc cataagcctt gccgcaccct caacgctgac gttcacacgg
     4201 ttgttggcat ttgcgcagaa agtgccgtcc ttgtcaaatg cggatatttc aatataaaca
     4261 aggtcagagc cgtctgcaag tatctctgtc ttgtcaggga cagcttctat tctaacagcg
     4321 tccccgaatg aacgctgtat atcctctgcg atgatatttc cattctcgtc atatgcaaca
     4381 gctttaagct cgcccttttc atatggtatg atatagttcg ctgtcagctc cttgccatgc
     4441 ttgtggtcaa tatcatatgc acccattgaa acaccgtcct tgaaaagctc aactctcgga
     4501 gcatttgagc aaactctcac gtcacaagga agtccgtcgc tgaaatccca gtatgggaaa
     4561 atatgcacga atgggtcgtc cttatagtcc gtccactctg cacggaaaac atatgcactg
     4621 tcttttgcaa agcctgctgt gtcgaactga ccaaagtagg agtttttcgt gctgtatgga
     4681 gtaggctcgc ctatgtagtc aaagcctgtc caaatgaact gacctgcaca gtattcagcg
     4741 tcatggtcag ggattatgca agcctcagta ttttttgcgc cccagcctgt gcagctattg
     4801 cccaaagatg aacactgctc gtcatcgtca gttagcaagg tttcagacag cgggaaatga
     4861 tatattccac ggctctgaac aacagaagcc gtttcgcttc cgtatatcat ccaatcaggg
     4921 tgagcggtgt ggtgttcctc ataaagtctt tcaccgtagt tatagcctgc aagcttgaga
     4981 atatcagcgc atttctgacc gttctcccac tgcatatagt tagagccgaa tgtgacatag
     5041 ccgttgccct cagggtcatg gagcctaacg agccttttaa gccaagccgt tacctcctga
     5101 cctctctcgt cagcgtgtgt gtcaaaaatc tcatttccca cactccagcc tatgatagaa
     5161 gggtgatttc tgtcacggcg cacccatgaa gcaacgtcct tttctaccca ctcatcaaaa
     5221 aatctggcat agtcatagtc ggtctttgan nnnnnnnnng gtctttgacc tttcccacat
     5281 atcaaagctc tcagagagaa tgagcatacc tgtttcgtca gcgatgtcca taaactcctc
     5341 cgcaggcata ttgtgagaag ttctgatagc gttgatgccc attttgcgta gttttttaaa
     5401 tcttctcctt atagccgcag gattggaaac tgccccaaga cagccgttgt catggtgttc
     5461 acatgagccg tggagcttaa cgtgtctgcc attgagataa aaaccgctgt cacacttgaa
     5521 tttgatagtc ctgaagccga atttctgact tacacgctga acacagccgc cgtccacaag
     5581 aatttccgac accatggtgt aaagcacagg ggaagtgata tcccaaagct cagggtcttt
     5641 cactgtcatg gtctgagcgg tgagagaata gctcacgcct ttcacacgca ccgcatcggg
     5701 tatgcaggaa atatccgccc cacaagcgtc agactcatag cttgccacct ttttgccgtc
     5761 cttgtcaaaa atagtgttcc tgatgcgaat tcccctaaga ggcgtgccat taggtcggag
     5821 catctcagcc ttcacagtca cctgccagcc gtcctcctgc tttgacgtgg tgatataaat
     5881 accgtcattg ccaaaacgta tctcagggct ttcgcaaagc caaacctttc tgaaaatgcc
     5941 tgctcctgaa taccacctgg agtttggcga gtggtgatcc acacggacag taataatgtt
     6001 ctcaccttcc ttaacaaggt cggtgatatc gaacataaat gccgaatagc cgtatttcca
     6061 ctcccctgcc aaagcaccgt tcacatacac cttgctgtcc atgtaaactc cgtcaaagcg
     6121 gataaaagtg cgtatgccct ctttcttttg gtaagaaaaa ttctttctgt accagcccgt
     6181 agaagtttca tataggtcat ttgtattata aataagccaa tcatgaggaa tgtccacagg
     6241 cttgaaagca aagtcctctg agtagtcaga gccgaaagcc gccttgtaaa attcccaatc
     6301 gttgcaaaaa agctttctgt ctgagtatgt ttcacagata tccataaaaa actcctttat
     6361 gttttactta tctgaatatg aaaatgtgcg tttatcaaag gcttttcaag ctgatatttc
     6421 tccgcaagcc ttggtccgca ggagttagaa cctacccctg ccattgcata gtcaacgcag
     6481 ataacattgc tctcgcacct ttcaagctca tagttatgtg cctttgccgc aagctcctcc
     6541 tgcgaaaact gagaagcgtt gaatgaaaac tccttgtcag cggtaaactt caccttagtg
     6601 tccccaccgc acacagtgag atatcgacag ccatagtgcg atgaattttc ctgcggtctt
     6661 atataatcct cgtgcatatc cccgatttca gccgcaaatc tgcccacata gcaagcctga
     6721 cgcttgtcaa tgtagctttc tgtaggacca tatccgaaat attccgccct tgaaaaatct
     6781 tttggcatga aaagccttat accgaacctt ggaagcatat ctatcttatt tgaaaactcc
     6841 gcttcgcatt ttatgtcaag cccactgccg tcaataacat aaacagcttt cagcctgcaa
     6901 aacggctgtt gtatggacca gccaaaagac tgtgtgacgc ttatctccgc tctgttctcg
     6961 ctcgctgaaa gctcacagcc gtagcttttc acatcaaagt cattaagatg caccttgtac
     7021 caattgtact tcatgacatc attatccgta ggcgcacgga agaagttata ctgcaaaggt
     7081 ctgtcaagaa gctctctgcc gccgaattcc acagaaataa acgccgactt acgcttgtca
     7141 aagcggtaaa caacatctcc cactgtcaca gtgatatcaa gaggcgtttc cacagcctca
     7201 acatttcctt tcacctcagc gaccttacaa gccttgccct ctgctatcct aagctgatca
     7261 aagcacacct catagccatt ttcacaatag tcagtgtcct cctttgcagt gaaaataaac
     7321 cttatgaatg tttcattttc atactccgct gtatcattgg gaacattcac cacagtgctt
     7381 cccataggct cgatgtcaaa atccaccctg ccgatatgta taataccgtt tttgtccgtt
     7441 atctcatagc ggcaatcgag aatatcccca gcgttcacaa actccagcgt gcttgaaaaa
     7501 acaaagcttc cctcgctttc cccttttgtt actctcacag gtctgtatac ctgctttacc
     7561 tctaaaagtc cggtgtgtgg cgttctgtca ggataaacaa gaccgtccat gcagaaattt
     7621 ccgtcattgt gtctttcccc aaaatctccg ccatagccgt attttatcct tccctcaacc
     7681 gtcttaccaa gcggtacaga atggtcgcac cactcccaca caaagccacc gcagaaacgc
     7741 tcattggaat agaacacatt gtgataatcc tccagatctc ccggaccatt tcccatggcg
     7801 tggcagtatt cacactgcac aagaggtctg ttttcctcag gcttttcaag atagcctttc
     7861 agcccgttta gatcccagta catacccgaa actacgtcaa gcacgctgtc ggaagttccg
     7921 tcaaggcagt gtgtgctttc ataatgcaca agccttgtat catcaagcct ctttataagc
     7981 tcagcttcat cgagcatatt tttgccatat ccgctctcat tacccaatga ccagaacacc
     8041 acacaagggc ggttgatgtc acgctttaca agacattccg cacgatcttt tatggcattg
     8101 ccaaacctct cgtcagaggc aagcatcgca ataccgttgt aatctgaata gtcccacttg
     8161 tagtcataat agacctccac acagccatgt gactccagat ccgcttcatc aataacataa
     8221 agccccagcc tgtcgcaaag ctgataaaac acaggggagt taggataatg tgacgttctc
     8281 acagcgttta tgttgtgctt tttcataagc acaagatcag ctttcatctg ctcataagag
     8341 gcataatatc ccgtgtcagg atagctgtca tgacgattta cacccttgaa cttcaccgcc
     8401 ctgccgttta tcctaacaac gcccttatcc acatttacat ctctgaagcc aatctcctcg
     8461 ccgataacct cgtcgcctgc attgatagta agcctgtaaa gataaggtgt ttcagcactc
     8521 caaagcttga cattctctaa ctttatctca acgctctcac cgtcataagc cgaaaactga
     8581 gccattgtaa caccgtcatc atcgcaaagc tttgccgaaa catcacagcc gaataccgta
     8641 aactccagcc ttccagagcc attttcaccg agaatagttt tcacgatata attttcaaga
     8701 cgcattttcg gtcttgacag cacataaaca tctctgaaaa ttcccgaaag ccttatcttg
     8761 tcctgatctt caagataagt tccgtcgcac catttcagca cagcggcagt aatgatattc
     8821 ctgccctcag caagatatgg tgtgatatta aactcagccg tagcgtgaga aacctgtgaa
     8881 tatcccacaa agctgtcgtt cacatacaaa taaacacaac tatccacgcc ctcgaacaca
     8941 agcaccctgt ccatgccgtc aggggaataa tcatattcac gctgataaac tcccacaggt
     9001 atatcgtcag gcacaaaagg cgggtcaaat ggtattggat agcacacgtt cgtatactgc
     9061 ggcttgtcat agccgtaaag ctgccagttt gacggcaccg ggatagtgtt ctcaaacttt
     9121 tcgtgaataa aatcgtcctc taggtctatt atgctgtcat aataacgaaa accccagttg
     9181 ccgtttaaaa gctccattcg ctgagaattt ttacggtcct caaaaggatc ctgccccttt
     9241 gcaaacggca caaaatagca gtgcttgtca agcgtgccaa tgtgcagtgc ctgagtatcc
     9301 tcatgatata tcattttcga tcttggcgaa ttttccgccg caagctttaa aatatccata
     9361 gctgttctcc tttacaagtt tataaattac tgtaattatt ttatcagcta ttgacaggca
     9421 agtcaatgaa tgatattatt aagatataac aatatgaata tggagttgat atttttgttc
     9481 tttgccgatt atcctatcct gggaaacgaa aagtccctgc cactatatct gcaaaatatg
     9541 ggtcatcatc actgtcagga tcaccttata agacccgagg gctatcacta tgaccagata
     9601 ctctactgca ccaaaggcag cggcacgctt atctttgaga ataaagccat ttccatacca
     9661 catgataccg ccatattcat acccgctgaa acgccccacg aatactatcc agaagaggat
     9721 atatgggata tccactggat agtcccctgc ggatatgccg ccaaggatat ccttacccag
     9781 ttcggcctta ccgagcttaa aacctttcac ctcagcgaca cgaaaatgct cgagcatatt
     9841 ttccgcaaaa tgcacaatgc tctgcgaacc gacagcattt tcggcaatta caaagcctct
     9901 ggctatttgt atgattttct tatggagttt taccgcctca tcgcctacaa agaccctgcc
     9961 acgtcagtat cctccgccct tatgaaagca ctggactaca taaactacaa ctatttttcc
    10021 cctatcacaa tggacgagct ttgcagtgtg tcaggagtaa ctaaacagca cctttgtctg
    10081 ctttttcgca aaaaactcag tatgcgtcca atggaataca tcgcaaaacg tcgtatccaa
    10141 gaagccaaag ccctgcttac cggtacagaa aaaagcatcg agcaggtagc cgaggaaaca
    10201 ggcttttgct cagaaagcta tttctgcaag cttttcaaaa gatacgaggg catgaccccc
    10261 tccgctttcc gccagatcaa ataatcacaa acaaaaaaag agccgcccaa aggcagctca
    10321 attatgcaga aaaattagcc ctgctccttc atcttagcga aacactcgct gcagtaaaca
    10381 ggtctgtcct ctcttggctt gaaaggaata acagcctcgc cgccgcaaga agcacaagtt
    10441 gctgtgtaag tctgtctttc agactttgta gcagcctttc ttgcatctct gcaagcttta
    10501 catctctgtg gctcatttac gaaacctttc tcagcataga actcctgctc tcctgctgtg
    10561 aaaacgaact cgtttccgca ctccttacat actaatgtct tgtcttcgta catggttctt
    10621 acctcattac ataaaattgt gcctagtcgg atttgcaccg acacctttga ataaacaacg
    10681 ctctgctatt aagctatacg gccatattcc tatctcaaca gtgatctcaa tttccgtctg
    10741 actctctgca ttgcattgtc taccgccttt tccgacgtgc ccaagcctaa tgctatctga
    10801 ttgtatgaca ttcctctcaa aaacgattga aatacctgcc actcctgctc cgaaagtgca
    10861 gaagtgatct tatcataaag ctcattcagc tcctcacgct ccactacgac ttcctcaggt
    10921 acagtgccac cgccgccaag aatagcctca aggtcagctt cctcgccgcc tgaaagaagt
    10981 gcatttctgt gaacagacga aatgatctta ttttttatac attttgtagc gtaagttgaa
    11041 aacttcgcat ttttgtcttc acgataggtc ctaaccgcac ccagaagccc cataagcccc
    11101 tcttggacaa gatcttcatg aacctcggcg ccatattttt ttgccagaaa ctccaccgta
    11161 cagatatatc tcgcaataag ctcaccgata gccattttgt cactctgtgc atttagcacg
    11221 agctgctcat ctgaaagctt ttcctgctga tcctgccgat cgcctctcaa aaaataccgc
    11281 ccacctaatc gtagaaatta ccgcaaagcc ccattctgac gccactgcgt aatgttataa
    11341 taccatttta gaccataaaa gtcaagagaa ttgacgaaat ccgccgtccc tgtgaaaata
    11401 tttgtaacat accacagttt taagaatgta ttcttgtgat tttcaacaaa caaaaagtca
    11461 gccgtgtcga aatacacggc tgaccgcaat ttacgcaact ttgatgttat actcggcagt
    11521 attttgctct gccatcgctg aaaatatccc ctccgtggca atcgtcagcc acaacaagag
    11581 gaaaatcctc aacgtaaagt tttttcacag attcacagcc cagctcctca aaagccacta
    11641 cctcacaaga ctttatgcag ttgcaggcaa gtgcgcccgc accgcctata gcacagagat
    11701 agaccgcacc atttcttatc acggcgtccc ttacctcttg tgatctctcg cccttgccga
    11761 tcattccgcc caatccaaga tcaagcaatc tcggtgcaaa cctgtccatt cttcctgaag
    11821 tggtcggtcc gcatgaaccg ataggctttc cctcaggagc aggagtaggt ccggcatagt
    11881 agataacagc gtctttcaaa gggataggaa gctcctttcc ctcgtcaagc aaagcattga
    11941 atcttttatg tgcagcgtcc cttgcagtgt acacaacgcc cgaaagcaat atcttgtcac
    12001 cgcatttaag ctctccgctc accgctttaa gctcctcgga acgtaccttt cttatttccg
    12061 acatgacaaa ttgttattca gcgtcatcat cgcttacaag agaaagacca tcgtcaataa
    12121 gatctcttgc agtgtttaca gcaccgatgc tattgccata cagatcgtta agtgatgtaa
    12181 cgagctgctg aacagtttcg ctcagacttg tgaactgtgt cttaacagac tctctcagat
    12241 cagcagactg tgctctgatg ttcttagctt cgttcttagc gtcgcttact acctttgcag
    12301 cctcagcatt tgcctttgat gttacgtcag cggcttctgc gttagcctta gatgtaacag
    12361 cctctgcctc tgcgttagcc ttagaagtta cctcggcagc ctgcttgttt gcagcacttg
    12421 taacagcttc agcctcctgc ttagcggcag ccgtcattgc agcagcgctt gacttagctg
    12481 tctgaagtgt agtatcagcc tctgtctgag catttgtaac aagcttctgt gcctgtgcgt
    12541 ttgcgtcagc aagaacctga tcctgaagct gcttagcttc atcttccatc tgcttagctg
    12601 ccttcttagc ttcctgaacg accttattag ctgtgttctg agcttccatg aacacgcttc
    12661 caagatcgaa tgcaggagtg gctgtgccgg cattagcctc agcagtttcg agcttctctt
    12721 taagatcctc gatctcctgc ttctgctttt caagctcagc gtccttttca gttacctgct
    12781 gctcatagtc agcgatacga accttcattg tatcagtatt agccataagc tcggctatct
    12841 tgtttttagc ctcttctacc tttttctcaa gttccttctt gccctcgaag tcctgcttct
    12901 gagcgtcgcc ctgcttctca agacgttcga tagtttcgtt ctggtcattg acctgagcct
    12961 caagagtata aattttagaa gtaaggtcgt ctacataagc aagaacgtcc tgcttatcaa
    13021 atccgccgac tctaaccgtt cttaaataac cattactagc catttcaatc cctccacatt
    13081 agtgattatc tctattatac aataaaccac gttatttttc aactgattta agccgttttc
    13141 aaaaatttca ttattccata caaaatagca actgaaattt atacaaaatg tcttaaaatc
    13201 atatttatca cagaataatt atatcaaatt ttccgtaaga tttcaagtgc ttttgcgaaa
    13261 aattttcaat atcatgataa ttttctgaga atacgaaaat ctctttaatt atgtaaacgt
    13321 tttccgactt tggttcaaac aaggcggaca gcgtaaacgc cgatccgcct ttgaaagcat
    13381 tattacttgt tcttgaagat attgaagata tcgtcgtaat aatttggatt atcagcagcc
    13441 ttaacgccag ccttgtgcat cgcttcagcg tcagcctttg cgtcgtcagc cttctgagta
    13501 aaatttgcag tatatgttgt cgcagccttt ggtgcagcag cctcaggaac gtcatctctg
    13561 tcaacaaagt cagcagcgat aacagtaacg tccatagcgt ccttggtatc aggatttgta
    13621 ccgttaccaa aaatgatctc aacgttatcg tcagcagcgt ctgctatagc agctgtaagc
    13681 tcgtccatat cactgataag gagatcctcg ctcattgtaa cgtttacgat aagtcttctc
    13741 gcacccttga tagaagtttc aagaaggtcg gaagaaataa cctgattgat gatatcctgt
    13801 accttatcct tgccctcgcc gtgaccgata gcaatatgcg ctctgcctgc acccttaaga
    13861 atagtagtaa cgtcagcgaa gtcaacgttg atctcatcat gtcttgtaat gatctcagca
    13921 atagcgataa cgtctgtctt aagaacttca tcagcgatag cataagactg cttcatagtg
    13981 aggcgcatct cgtttgtgat aaggttctgg ttagggataa cgataagagc gtcaacgtgc
    14041 ttgataagag cctcgatacc cttttctgct ctctccatct tcttagcgcc ctcaaatttg
    14101 aaaggctttg taacaactgc gatagtaagc ttgtcaagtg ac
//
LOCUS       DAWEMR010000054        14135 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1783_length_14135_cov_10.289062,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000054 DAWEMR010000000
VERSION     DAWEMR010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14135)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14135)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14135
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1783_length_14135_cov_10.289062"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1621..1630
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3369..3378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13622..13631
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4199 a         2969 c         3225 g         3712 t
ORIGIN      
        1 ttctaatttc gtatgatgta tcagtataat aataggatat atcattgttg tttttttgtc
       61 aagtgataaa atataattga aactaaaatc atatacatca aggaggtcac aatgaaagag
      121 atttcaatgc ttatcaatac cgcagacaaa cgttcccaca tcaacccgga gatatacgga
      181 cacttctccg aacatcttgg cagatgtatc tataacggaa tgtacgttgg cgaaaattct
      241 gagataccga acataaaagg cgtaagaaaa gacgttatcg aagctttcaa ggagataaaa
      301 atgcctgttc tccgctggcc gggaggctgt tttgctgacg agtatcattg gaaagacggt
      361 atcggcgaaa aatcccagag aaagaaaatg ataaacacaa actggggcgg cgtgacggag
      421 gataactctt tcggcacaaa tgagttcttt gacctgtgcg aagagatagg ctgtgaaccg
      481 tatcttgcag gaaatctcgg aagcggcact gttcaggagc tttccgagtg gatagaatat
      541 atcacctttg acggtgtttc gcctatggct gacctgagaa gaaaaaacgg cagagaaaag
      601 ccttggaagc tgaaatatct tggtataggc aacgaaaact ggggctgcgg cggctcgatg
      661 cgccctgagt attactgcga tgaatacaga agatatcaga gcttttgcaa gaatttcagc
      721 ggcaatgagc tttacaaaat tgcctgcgga ccaaattcca atgattataa ctggacggac
      781 gttgtaatgt caaaccttgg ggagtggcac acaaaggcta tctctttgca ctattacacc
      841 gttcctacag gagattggaa caaaaagggc agtgcaactg agttttcaga cgaggagtat
      901 tacgaaacta tatcaaaaac tctatatatg gacgagctta taaaacgtca cagcgagata
      961 atgtcacgcc gtgatccaga gggtaagata ggtcttatag ttgacgagtg gggctgttgg
     1021 tatgacgttg aggagggcac aaacccaggt ttcctctatc agcagaacac aatgcgtgac
     1081 gctatcgtgg cggcttgcaa tttgaacatc ttcaataacc acagcgacaa ggtcgtaatg
     1141 gcaaaccttg cgcaggctgt gaacgttttg caggctctta tcctcacaga gggcgagaaa
     1201 atggtgaaga cgcctaccta tcacgtcttt gatcttttca aaactcatca gggaggcgag
     1261 ctttgctaca gctatgccga caattgtcag gcaggcggaa agatcccgat gatatcacaa
     1321 tctgcctcag taaaggacgg cgtcctcact gtcactatag cgaactgctc tatagatgat
     1381 gacgctcata tctgctgtga tatagcagga gaaaattatc agaacattga gtgcaggata
     1441 cttgacaatg aagtaaggga ctataatgat tttgataact gcgataaggt cgcaccaaaa
     1501 gagtacagtg cgaaaatgac agaaaaggga tttgagatag acctgccaaa atgctccgtt
     1561 gtagttgtga caatgagata aggactataa ggagagtcaa aaatgacagt aaaagataaa
     1621 nnnnnnnnnn tctgttatcg catcagggtc gaagcagtgg gaaaaccgct ttgagaacgg
     1681 ctgttggaca tattctcttg atgatatcca cgggggcata caagcctgct ataaagacct
     1741 tgcagagaac gtaaaggcac agtatggcat ttatcctgaa acatacggtg caatgggtat
     1801 ttctgctatg atgcacggct atatggcttt tgacaaaaac gataaactgc ttgtgccttt
     1861 cagaacatgg cgcaatacca tgacagctca ggcggcagga gagcttacaa aactactggg
     1921 cttcaatatc ccacagcgtt ggagcatcgc acatctttat caggcggtgc ttaacaaaga
     1981 agcccatgtt ccttatatcg cttatatcaa tacccttgca ggctatgtgc ataaaaggct
     2041 tacagaccgc tttgaagttg gcgttggcga agcttcaggt atcttccctg tggagagcat
     2101 aggatacaaa aaagagtatt tggagcctac agaaaaaagc cttgcagaaa aaggctttga
     2161 ccataagctg aaagatatcc tcccagcggt aagaaatgca ggggaaaagg gcgctgtcct
     2221 tacagaagag ggagcaaaat tccttgaccc ggagggacgc cttaaagcag gcgtgccggt
     2281 ctgcccgcct gagggtgacg caggcacagg catggtggca acggacagcg ttctgaaaaa
     2341 gaccggaaac atctccgcag gcacttccat atttgcaatg ctcgtcctcg aaaagccact
     2401 taacggtgtt tacgaggaga tagacgttgt taccacccct gacggctcgc ctgtggcaat
     2461 ggtgcattgc aataactgct gttcagagct tgacgcatgg gtgaatatgt tcggtgagtt
     2521 cgcaaagctc acaggcaatg agatagacaa gagcaggctt tacgaaacgc tctataaaaa
     2581 cgccatgaca gccgacagct catgcggtgg cgtgacagcc tataactgtc tttcgggtga
     2641 acctgttata aagcttgacg agggcagacc aatgtatttc cgcactcccg acagcacttt
     2701 gaccttgggc agctttttca gagcacagct ttattcttct ttcaccgcac tgaaaatagg
     2761 catggatatc ctctttgaca aagagcaggt aaaagctgaa aagataacag gtcacggagg
     2821 acttttcaag gtcagcggag tggcttcaca gcttatggca gacgcacttt ctacccctgt
     2881 ttcagtaatg aaaacagcag gcgaaggagg agcatggggc atggctatcc ttgcggcgta
     2941 tatggagctt tcaaatggcg aaaagcttgc agacttcctt gacaaaaagg tgttcgctga
     3001 tatggaagtg acaacttctc agcctgagca gacaggctca gagggttata aaacattcct
     3061 agaaagatat aaaaagggat tggcggcaga aaaaaacgca cagttctgaa aaaatcatag
     3121 aaattcatag atagatcgta ggggcgacct gagatcgccc gccaaccaac agtcttctca
     3181 aattgtcggt gagctattca cttggacgga aaatgggcag acaaaaggtg catttaccaa
     3241 atcagcgaaa ggagaatttt atgcttgaag aattaaaaca gcaggtgtgc gaagcaaatc
     3301 tgcttctgcc gaaatatgga cttgtcacat tcacatgggg aaatgtttct gctatcgaca
     3361 gagaaaagnn nnnnnnnntt gtagtcataa agccctcagg cgtgccttat gagggcatga
     3421 cagccgatga tatggtggta gttgatcttg aaagcggtaa ggttgtagag ggaaaatata
     3481 agccttccag cgatactcca acacatttgg agctttacaa agcctttgat atcggcggaa
     3541 tagtacatac ccacagtcgc tgggcaacat cttttgctca ggcaggaaag ggcataatac
     3601 caatgggaac aactcacgca gattattttt acggcgagat accatgcaca agagcaatga
     3661 cccctgagga gataacaggt gagtatgaaa aggaaacagg tactgttata atcgaggctt
     3721 tcaaaggtct tgatgcaact cagatacctg ccgttctggt aaaaagccat ggtccgttca
     3781 catggggaaa ggacgctgca gaagccgttc ataatgcagt cgttctcgaa gaagctgcgt
     3841 tcatgaattt ccacgcaatg atgctgaacc ccgagatagg tcatatgcag caggagcttc
     3901 ttgacaagca cttccttaga aagcacggaa agaacgcata ttacggacag aattgatgtc
     3961 aatgcctcaa aaagcatgat ataagtccgt tgaaaaaatg agtcaaataa cgggcgaaca
     4021 ctgttcgccc ctacatataa aagatttgtt tagcacatcc tcgataaaat agcgccccga
     4081 ttttctgggc gtttttttgt gcctttgctc acatattgtg caaaatgtat attatgtgta
     4141 cgctgatttg tacaaaaggg tagttttttg tacttgttat aagtaaattc ttgacttatg
     4201 ttttaataat atgttataat taaaataatg tcacggtaat atttattgtg tcacagcgga
     4261 aatgaatgga atatagtata gatattacct caaggcaatg ccatgcaagg tttgccttag
     4321 aaacgaggaa cggaggcttg taatgaaaaa gcaaattgat tacggcagct atgcgtccta
     4381 taaggaaacg gtaaagggtc ttacagagaa cctgaaatcg ctgcttggat tgagcgagga
     4441 gatcgctctt gtcaatactg cggaatctat tcgggaaaca ttggagaaaa ctgccgagga
     4501 gcattttgag gtcgctatag tgggcgagtt caagagagga aagagtaccc ttatcaacgc
     4561 aatgctgggt caggaggtac tccctgctga cgtgctgcct gcaacggcta cccttaacag
     4621 agttacttat agcgaagagc cgtatgtgca ggtggaatac aaaaacggca aaagtgaaag
     4681 ggtggatata aacaagctgg aggactatgt aacaaagctt acagcagagt cggaggaaag
     4741 agccgaaact gtcaaggaag ccactgttta ttatgatacc gagttctgta agaacaacgt
     4801 ggatatcata gacactccgg gtcttaacga tgacgaccag atgaccaacg ttactctgtc
     4861 tatcctgccg aaaatagacg cagccgtttt cgttatcagt gcaaactccc ctttctcaca
     4921 gtttgagaaa gagtttttgg aaaagaaaat gctcacttcc gatgtgggaa ggattatttt
     4981 cgttgtaaac tgcttcggta ctttctcaca ggcagacgag aacaagatag tcgaaactgt
     5041 aagaggacgt atcggaaaat acgttatgga aaaggctaaa aaggtcatgg gcgaggattc
     5101 ccgtgagttt gcggtctata aaagaaagat aggaactccg agagttatcg gcgtttatgc
     5161 aaagcaggct cttaatgcca aggaaacagg ggataaggaa gcccttgaaa agagtaactt
     5221 ccctgagttt gaaaaggctc ttgaaaccat gctcacaaaa gagcgtggcg ttatcgcatt
     5281 gcagatcctt gcaaataaga taacaaattc gggtactgag atactcagaa gcgttgttat
     5341 gcaggaaaac gcactcatga tggctaacga tgagttcatg gaaaagtatg acgaggctat
     5401 caaagagata ggcgagataa gaaataagaa aagacaggaa tttgtcaaga tcaacgacgc
     5461 cgcaaatcag gtgttcaatg acctgcagcc ggttctggac agctattgga caaacatcga
     5521 gcagacagct atggaggtta tagacaactt ccagatgtcc tctgacgatt tcaagaaaga
     5581 ccagctcaag ctcgttaccg caaagctcac cgagaaaata aaggagagca tagaggcaag
     5641 agcgcagata atctgcgagc agatacagaa cagcataaac ttcgctgttt cctgtgaggc
     5701 tgaaagactt caggaatttg aggacgagtt cttcgagtcc gttacaaaga tccagcagat
     5761 gttcactatc tccgacagag tttcaaagaa cggcggcgta atggataaga taatcggcgt
     5821 tgctatcggc tcttacggcg tcggaggatt gttcctgggc ttcagagagt ccggcgtaaa
     5881 gggcgcactt ttgggcggag caacaggctt tgcaggattt tatgctactt attatactgc
     5941 tatattcttt gcaggcttct ttggcatcac cgctaccctg ccagtgaccc tctgccttat
     6001 ggctcttgca ggcattgcag gtacttttac ttcaaacttt gccgttgata agatacttgt
     6061 taaggaaaga atagataagt ataagtcaaa ctttaaaact caggtgagaa agcagttcca
     6121 cgaaatgaag ctcaataatg acttcactga aactgtccgc cgtcaggtat tctcagcatt
     6181 tgagggtctg aagggcaaga tcgagagcga aacagagata atcctccgtg atactcagga
     6241 aacacttgat aatcttaatg atcttaaaac agaaaagagc aacatttctc agaaggaaat
     6301 ggaacgtctg catgatattg ccgagagcgc aagcgctata gttcaggaag catatcaggt
     6361 aagacgtgtg cttaccgaag ctatggaaag ctgacggtca tataaaaata ttattttagg
     6421 atcaagggag gacaggtcat gagtacaaat acaacggaaa gggaaaatcc gctgctacag
     6481 attgacacaa gcttccgcac atatttgaac gcttatacaa gaagctatca gaaccatata
     6541 gttgacggcg tgcttgacta tgcttttgag tccgactttg ccgtaaggca gaagataatg
     6601 ggacttgggg gctggggaaa gctcgtcaag gcggcaggct cgcaggacgt ttctgccgaa
     6661 gcaaagcacc tgtttcttaa gtgcgagcag gttggcccgc tgaaatatcc tgatatctat
     6721 gacattgtga agaagtgtgc cgagcggcta gagcttgttg tgcctatcgt ctttgtcaga
     6781 ggggatatgg ataaagcaca ggtctactct gtggcaagcg atatcatcga gccttgtatc
     6841 gtgctttcaa agcaggtagt ggaaatgtgc agcaaggagg agcttatgtt ccttataggc
     6901 tgcgagtgtg gaagaataca gaacaatcat tgtgcctata atatggcatt tacatatctc
     6961 aattacaata agtatactta cagaccagtt gaaaggtcat ataagcagac tgtgaacaat
     7021 cagctttata cagcgcttgt tcagtgggtg aagtatgctg atataactgc aaacagagca
     7081 ggcattatct gcctcgataa gccgggaatg ttcataagcg tcataacagg gctttataac
     7141 aagggctata tcgactttta tggcagacag cagaagaata tggataccga cggacttatc
     7201 aaaaaggctg agagcgtcca tgcagtttct tcaagaaatc tgaaaacaga taatactatg
     7261 agcgagcttg aaaagcttgt tgtcgcagct actgagtttc tgtattgctc agttctttat
     7321 gaatggcgtg gcgacgcaga ggattctgac agacaccttg tttcaggtca gatatgtgat
     7381 gtgcgcagca gtattataat cggaaacgga ggcgtgatag gtggatagtg ctaatatcaa
     7441 tacagcggag cagaatattg cacaggtaaa gaccgctctt aaagcctttg ttgaaaatga
     7501 aaagatagcg aaaatgctcg gcaaggattt tgtcagcgaa atgaacgatt gggaagctct
     7561 tatcgagaaa aaaagcgaag agcctttcac gctggtcata cttggcgagt tcaaaagggg
     7621 aaaaagcacc attataaacg ccctgctggg caaggagctt gcacctataa acgttacacc
     7681 tgaaacttat actataaatg agatatcttt cggtcataca cagtatgtgg aagctatcct
     7741 tgaaaacggc atgagagtgc cgctgtcgct ggaggatatt acaagagaaa agcttgagca
     7801 gagaatgaag cttttccctg caaaaataag ctatgttcag ataaaggaca acgcccctat
     7861 actcaaagag ataaggatag tggatacccc aggcctttct gacttggaga gccttgacaa
     7921 gcaggtaatg gattatattg tcaatgccga cgctatcatg tatgcggcat catgcctgct
     7981 gcctttttct gaaagcgagc agttgttcct tgcaagccat gtccagccgc agaggttcgg
     8041 tatgctctat atcctcgtga atatgataga cgcaatgaac tctcagaagg acgctgataa
     8101 aatactaaag cgtgtgagaa acatatctga gaggatagtg cctaacgcta tcgtatacgg
     8161 cataagcggt gcagacgagc ttagccgaaa gcttggcaga gaaagaccgc tggacaaggg
     8221 tacaagagag ttttacgaaa ctcagttttt ccagttcgag ctgtctttgc aacgtgatat
     8281 aatcatgcag aaggacgtta tacgttcaaa gcgtgttctt acaatgcttt cgcagatgtg
     8341 cgatgaaaca gccgcaaagc ttagacttat aagcgatatg gcagagcttg acaagcagaa
     8401 gcttgccgac agagcaaaag agtttgagga gcagtgtgaa aagctttcaa aggctctgga
     8461 ggagaaaaag cctgctttgc acctttctat tatcgaaatg cagcaggaag ccgaagcttg
     8521 gatgtatgag ttcttctcaa agctgagagc aagcattttg gattgccgtg gaaaagatgc
     8581 agacggaaac gacctttaca gctctgagga tatagaaaag catttctatt cgttccttat
     8641 ggaaaaggtg ggcgaagcat atcgaaattg tatcgagagc catagagata ggatagccga
     8701 gatagtggaa ggcacaggca gagagcttgc ctccgccctt ggcatagacg atctttcaaa
     8761 ggcggcaaag gctccgtcag tggaaatgat aatgacttct ctgaacaaga acgtaacacg
     8821 ttctgttatg ggggtgcagc tttttggcac cagcgaaacc tttcctcctg ccacaatgtc
     8881 agttttcagc cttatcctta aaaagaaaaa gcagaccgat ataattgata tcgctcttga
     8941 aaactatgac gatatcagaa ataatatcgt caaggatata aagacagttt atcaggacct
     9001 tgaaacaaag gcagccgcaa ggctcgacag cctttatcag tatcaggtgg aaatgggcag
     9061 agaagccctc aatcaggctc agcagctcat gatggtttat gataatgacg agataaggca
     9121 gacccttgtg gaagcaaagc aaatgcttgc cgagccacag agaatactgg cagagagcaa
     9181 attataacaa aaataaaaac gtcccattaa gttggggcgt tttttttgat aatggtgtaa
     9241 atatgcttct tgacaaaaaa acaggctgtt tttgcagcct gtttggaata tctaaaaaag
     9301 tatcagatca acgggcgacc tcaggtcgcc gctacatttt gtatgcggta aattatttgt
     9361 ctttgtattg ctcaacattg ttttctatct tgtcaacaga gtttctttct gttatgtcaa
     9421 cacgatagtt gtactgagcg ttctttgtga cagtgttttc agtgaagtat tcaagttcac
     9481 cgtctttatc gaaaaaatag gttgtggaaa agccctttgt gcctatctcc tcagcgtcct
     9541 ttcccagctt tgaaacgtca taaacatacg tcacagtaac agagccgtca tcaccgataa
     9601 tgtcagaaac agactcaaca gctttcggtg caaacatcag cataccctcg tcagcttgtg
     9661 ggtgctttgc ctttcttgtg tactttgaaa agtccttgtc gccctttacc aaatgtgagt
     9721 attctccgtt atcataggtg tagctctcga ttccgttgtt atactgagcg tattcctttc
     9781 caccgttctt gccgtagtaa gaataggtca gcacatcctt ttcgtcatac ttgaaagtga
     9841 actcctgctc gatatcacca gtgtcctcgt tggttataac gacttttgcg gagtcaagcg
     9901 ccgcatagtc ctcccttgcc tgatatacca atgtgtcatg ctttagctca ggcgtaccgc
     9961 aggaagtgca taaaaggcag acagccactg ttaaagctac tgctgctgtt tttagaaatc
    10021 tcattttaaa atttactctc cttaaagccc cttgcaggtc tttgagaaca ctataagcgc
    10081 cgcagcaata gtgaatatct gcgagatagt tgcatggacc ataagaccta tattgaattt
    10141 tatcacatac aggtctatag caaagttttc aacgcctaca ttttttcccc atgcaagcca
    10201 gcccacataa tccttgccct cacagacgta agctatgaac gcaccaagta ttattcctgc
    10261 cagcatgaaa aatataaacg caaaagtctt tttgaaattt ttcatgactg aatatcctct
    10321 gttttatgta tcggtatcta ttttcttctt gacggagatt ttctcttgtt gtcaatgact
    10381 tttttcagat cagaaaatct ctcctcgctt gacgccttga acttgctgag catatcctca
    10441 aaatcagcgt ttcctgaatt gctgtcagca ttgtataccg gaggcggatt tttagcaaaa
    10501 tcctgtggcg ctctgtctct gttgtgatcc ctgttatagt cgtttctagg tctgccctga
    10561 gaattgcctc cctgacggtt gaaatttctc tgaccaccgt taggtctgcc ctctctgcca
    10621 ccctggtgac cgttctctct tggccttctc tgtggcgcag gctgagcctt cttgatagaa
    10681 agcgatatct ttccgtcgtc gcctaaatta agcaccttta ccttgacggt ctgaccctcg
    10741 gtaagatgat cctttatctc gtttacgaat gtgttcgcaa cctccgagat gtggaccatt
    10801 cctgtcgtat ccttgtcaag ctccacaaat gctccgaatt ttgtgatacc tgttactttt
    10861 ccttcataaa tttttcctac ttcaagctgc atttgatctt tgtgtcctct ctgcaataaa
    10921 aattctgcta tatgaacggt caaaagaccg tgtttgcctt agtcgctgtt gtttacaata
    10981 taaaatctgc gctcattagg ataagcatag cccagctttt caatagctat cttctccata
    11041 tactccgcct cgtcgtcaga actgagcagc cttgtgtatt catcattgag ctgcttggcg
    11101 gcggttatct ttttatttat agcgtctgac tcttttctga gctgtgctat ctgcgcctgc
    11161 tgtgagatga taatcacccc tgaataaaca acaagagcgg caacagcaaa aatagtcaga
    11221 agcttaacaa atcttgatct tttcggcttt ggattttttt tagcagcgtc cttttcagcg
    11281 tcagccgttt cggacgtttt atttttaaaa cctagcattg cgtactcctt ttttgattac
    11341 aaaaccgacc tttccataag caatacccca ataactgtca tgaggcgtta cctatactct
    11401 tttctattat acactaatca aaagagagtt ttcaagtgct tttagaagtt ttttttcgca
    11461 gagaggaaat tttgcacttt ttcacaaaac agtccctgaa accgtctgct cttttggtaa
    11521 taaatttaac cacaggcgaa aacgttttag caaatagttt tctgatgatc gaaaaaaggc
    11581 tcctcagcac ggatacaaca gcgtttccta cagtaagatg ttctatcaca gagcctgtta
    11641 gcataccgac taaaacaaac cctcttatag cccccgtcag tccggtgcaa aatgtgaaaa
    11701 gtgcaaagcc gtaaagaaat gtgtaaatga tatcctcaaa aaaggtgagt atcttcccat
    11761 gtggcaaagc cgtcctgaat accctgaaaa tatcatataa aagccccagc ccgcagccca
    11821 gaaccacaga cagcagaaaa agctcccttt caagctctat gcttaactgc atcttttcca
    11881 tttatctttc ccctttctcc aacctcttgc accatgcaat gacattgcat atacttttgg
    11941 cttaaaaacc gcccgaatat acacccccca cttgtagggg cgaacagcgt tcgcccgcct
    12001 gttcgcagtt ttttcggtta atagctatcc aacaaaaatg cggatatacc gaccacaatt
    12061 agcggtcagc caacgggtga cctaaggtcg cccttacata tgttatctga atatcttccc
    12121 tatcgccgag agctttttct ttctgtcctt ttctccataa accaaagccc agatatctcc
    12181 ctccacggaa acatctcccg actcaacgct tatttcgttt atgtgcaggt ctttgccttt
    12241 tatcgtcagc tctccaagct gtgtgaaaag ccttatctca tcctcgtcaa agctgtcaac
    12301 gtccgttacg cccgaaagct gtaaacgctg tctgttttca agaatggcat tgtgcctgcc
    12361 ctgagtatca ttcataaaag caccccctga tatttttctt atctatgtat attcgctccg
    12421 tgtaaaaata tttctctgaa aacgttttat atccttgaca aaaacgcctt gaaaagctaa
    12481 aataaacata tgcaaaaaaa tcatagtatc gaaaggatat gaaataatgg cagagaataa
    12541 aaagcttata acagcagaca acagctttct tacttatatg ggcagaatag acttttccga
    12601 caaaacacgc cctgagtttt attacgcagg ctcaaatgta aaaatgactt tcacaggcac
    12661 aagcgtatcc gccgtgataa aaaaccataa gtatttcaac attcaggagc ttggctttgt
    12721 tatggacggc aaagagggca aggtgacttt cgataaaaat gacgaggata tcacccttga
    12781 tatcgcttgt gaccttgaaa aaggcactca tacagtcacc ctttttaaaa gacaggacgc
    12841 ctcccattac tttgagttcg tgggctttga gatagacccc gacggagaag ttcttgaaaa
    12901 tcctcctctc cctgcaagaa aaatagagtg ctatggcgac tctgtttctg caggcgcagt
    12961 gtgcgaggca gttgactttg ttgcaagctg tgaccctgag gatcatcagg gagtttacga
    13021 taacgcatgg catagctatg caatgataac agcaagaaac cttggcgcac agatccataa
    13081 tatcgcacag ggcggtatcg caattttcga caacactggc tattatcatg caccgaatta
    13141 tatcggcatg gagtctgtat acaataagct ttgctatttc ccagagggcg caaagggcgt
    13201 aacaccttgg gattttgaga attatacccc tgacgttgtg ctttttgcag tgggtcagaa
    13261 cgacccgcat aacgagggtc acgaggattt cgatatcgcc gatcccgatt acagagcaaa
    13321 gtggaaaaat gcctacaaga gcattatcct tgaccttaga agcaagtatc caaacgcaac
    13381 atttgtgctt ctgctgacag ttctctgcca cggtgaggag tgggataaag ccgttgacga
    13441 gatagcaaac gagcttaatg accagaagat aacacatttc atgttcacaa gaacaggcaa
    13501 ggcgaccccc ggtcacccaa gacttcctga gcagtatgaa atggcggagg agcttacagc
    13561 ttatctctca aacatgggcg ataaaatttg gggctaaaat ctgaaaaaat aatagggcga
    13621 annnnnnnnn ntgttcgccc gtttttttta tgcctgtttt attggctttt ttatcgtttt
    13681 cagcttaaat cctgctgttc cgccgcctgc ctgtcaagct ccatgcggta agcagaaagt
    13741 atctctccct ctatctcctt gcgaagctcg ctgtcgatgg ggtgaacgat atcacggaaa
    13801 accccgttgt catcacggcg gctgggcata gccacaaaaa gtcgttcctc tccttgcact
    13861 atcttgatat catgtaccgc aaaactgccg tcaaaggtca ggctcacaac ggctctgagc
    13921 ttgccctcgt ttataaatct tctgaatttt acctctgtta tagtcatttt catgcgtcct
    13981 ttcttgtgat gtaatgcctg tagtaatatt tttgcctctt tttttgaaaa ataatcaaaa
    14041 aaggggttta ttttttttta gaaatgttat ataataaaat aaacatacat tctgaattat
    14101 ctattattct aaggagtatt acaaagatga taaca
//
LOCUS       DAWEMR010000055        14002 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1801_length_14002_cov_8.501972, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000055 DAWEMR010000000
VERSION     DAWEMR010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14002)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14002)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14002
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1801_length_14002_cov_8.501972"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3914..3923
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4518..4529
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7931..7940
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10017..10026
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3510 a         3319 c         2875 g         4256 t
ORIGIN      
        1 ctcgcttcct tttcttttca gatagtctgc aagaattcct ttttctatac attcggctgc
       61 aaggtaaacc aatatgaaac agaaaatata aaggaagcaa tgctgtccga ggggtatgag
      121 gtcactgagg actatgctga tgcagaggtc tgtgttgtaa atacctgcac tgttactgct
      181 cagtcggact caaaatgccg acagcttatc cacaagataa aaaaagccaa tcctgagtgt
      241 ctgatagttt tggcaggctg ttttccgcag gcttttgaaa aggaagcttc tgctcttgac
      301 gagtgcggta tcatagttgg cacaggtgca aaaaaggaga tacctgtcct tataaaaagg
      361 tatattacaa gtggcgagcg tattatcagg atagttcctc gtgagagggg agaggctttt
      421 gagaaaatga caaactcagg tgctgaccaa aaaactcgtg cgtatatcaa aattcaagac
      481 ggctgtgatc agtattgcac ctactgtatt atccccactg cgagaggaca catcttctca
      541 aaacctttgg aggatatcgc tcccgaagtt cagcagctcg ttgactcagg tcacaaggag
      601 ataatcctca cgggcataaa tctttgctgt tacggcagag acttcaaaaa cggcacacgg
      661 ctcattgaag ctgttgaagc cgcctgcggc tgtgatggcg attacagagt gcgtatcggc
      721 tctgttgagc ctgagcttat ttcagatgaa gatatcctgc gaatgtcaaa gcttgaaaag
      781 ctctgtccgc attttcatct ttccctgcaa agtggctgtg acgaaacttt gaagcgcatg
      841 aaccgccatt atgatacggc ggagtatgct gagctttgcg agaagcttcg cgggtatttt
      901 ccaaactgcg ctataaccac cgacataatg gtgggctttc cacaggaaac tgacgaggaa
      961 tttgaaaagt cactggcatt tgcaaagaag atatcttttg cagaaacgca tattttcccg
     1021 tattcacgca gaccgacgac tgttgctgac aaaatgaaag gtcagctcga ccgcaagaca
     1081 aagcacagtc gtgcggctca aatggcagat gtatgtaacg aaacaaaggc tttgtatctc
     1141 aaaagccttg ttggcacagt gcaagaggtt ttatttgaaa aggaaacaag ccctcagtgg
     1201 catcagggtc atgcaccaaa ttatgttact gtgaaaattc caagggacag cgaggacgtt
     1261 tctttgcgcc gacagctttt aaagatagag ataacctcgt cagatggcga gttttgttac
     1321 ggaaaactta tatgagtaca aaaaagagga ccgcaaatgc gatcctcttt cgttatatca
     1381 gatttttctg cgctcagcca agctttgcaa gaattatctc tttcattttt ccagggttag
     1441 ccttgccctt tgaagccttc atgcactgac ctaccagata gcccagagca tttgtctttc
     1501 cgcccttgta gtcatttaca gacttttcgt ttgcggcaag aacttcgtca gcgatctttt
     1561 caagtgcgct gtcatcagaa acctgtgcaa gaccctgctc cttgataatg tcgcttacct
     1621 ttttgtcctc ttcaagaaga actgcaaaaa ctttctttcc gccagcgttg gaaatggtct
     1681 ttgcctcgat agcagcgacg agatcagtaa gattgtcagc agtgagcttt gtatctttga
     1741 gagtcttgcc tgttgcattc atgtacttaa caaggtcgcc aagtatccat gttgtatagc
     1801 actttgcagg gaacttgtcc tttgccatgc aatcgtcaaa aagctttgca agggaaacag
     1861 actcagctat aaacgttgcg tcctttatgt tcagaccgaa cttctttaca tatctgagta
     1921 tctttacgtt tggaagctct gggatactct ttttaagctc gtcaacacgc tcctgctcaa
     1981 caacgatagt gagcaggtcc ggctcaggga agtaacgata atcctgtgcg tcctcttttg
     2041 aacgcattgg aatgctcatt cccttttcgt cgtcccaacg gcgtgtttcc tgctcgacct
     2101 ttccgcctgc ttcaagaaca gctatctgtc tttctacctc gtagtctata cctcttacag
     2161 cggcagagaa gctgtttacg ttcttcatct cagaacgtgt gccgaatggc tcgccaggct
     2221 tgtgaacaga tacgtttaca tcacatctga tagagccttc ctgcatctta cagtcgcata
     2281 tgccaagata ctgtaggata gatttaagtg tttcaagata aacctttgct tcctgtgagg
     2341 aacgcatatc aggctcagaa actatctcga taagcggaac accgcagcgg ttaaggtcaa
     2401 caagtgatac accatggaac ttgtcagagt ggagaagctt acctgcgtcc tcctcgatgt
     2461 gtattcttgt gatacctatt cttctttcct cgccgtcaac aaggatatca acataaccct
     2521 tgccgcagag aggaacttct gcctgtgata tctgataagc ctttggcagg tcagggtaga
     2581 aatagttctt tctgtcctgc ttgcagacac ggtttatctc gcagttgagt gcgtgaccca
     2641 ttcttattgc atagtcaact accttttcgt taagagtagg caatgtgcca ggcataccca
     2701 tgcagatagg gcaaacctgt gaattgactt caagaccgaa ctggttcttg cagtcgcagt
     2761 atattttaga ctcggtatta agctcagcat gagtttcaag accgattaca acttcgtagc
     2821 cttttaccat ttttgtgacc cctttcctta aagctcaggc attttgaatc cgccaacaag
     2881 ctgctcataa gcataagctg tgttaagcag acgctgctct gtaaactttc cgccgataag
     2941 ctgcatacca acaggcagac ccttgtcgtc atatgcacaa ggaatgctta ttgcaggcag
     3001 acctgcaatg ttcactgtaa cagtgcagac atcgtttgcg tacattttaa ggggatcgtc
     3061 agtgttttca ccgatcttga aagctgttga cggaactgta ggagtagcga taacgtcaac
     3121 gtcagcgaac tgagatgcga actcctgcat tattctcttc tgaacgagct ttgctttctt
     3181 gtagtaagcg tcatagaaac ctgatgaaag cacgaatgtg ccgagcatta ttcttctctt
     3241 tacttcgtcg ccgaagccct ctgaacgtgt tctctcgtac atatcaacaa gtccgtcata
     3301 ttcttcggct ctgaagccgt atcttacacc gtcaaatctt gcaaggtttg aagaagcttc
     3361 tgcagaagaa atgatgtagt atgatgaaag tgcataatct gtagaaggca gagaaatctc
     3421 ctttacagtt gcgccctgct tttcaagctc tttcacagcg ttcattacag actgcttaac
     3481 gaatgggtcg ataccctcgc cgaaatactc ttttggtata ccgatcctca gaccctttac
     3541 gtcagcttta aggtcagccg cgaaatcagg atactctcta taggaagaag tagcgtcata
     3601 cttgtcatga ccaacgatag cggactgtac catagcaacg tcctttacgc acttgccgag
     3661 aggtccgatc tgatccagtg atgaagcgaa agcaacaaga ccatatcttg aaacgctgcc
     3721 gtatgtaggc ttcagaccta caacgccgca gtatgaagca ggctgtctga tagatccgcc
     3781 tgtatcagaa ccaagggtaa cgatagcctc tcttgctgca acagcggcag cagatccgcc
     3841 tgatgaaccg ccaggtatcc tctctgtatc aaatgggttt ttagtaggct taaaatgtga
     3901 gttctcggtt gatnnnnnnn nnncatggcg aactcgtcca tgttgagctt gcctgttaca
     3961 acgatatcgt caagcttgtt cattacagtt gcattgaaag gcggaacata attgccaagc
     4021 atttttgatg cacaagttgt tctcagaccc tttgttgaga tattgtcctt gataccaaca
     4081 ggtataccag ccaaaggaga aagctcctcg ccctttgcaa gcttttcatc tatctcagcg
     4141 gcttttttca tagcgttttc ttcgtcaagt gtcagataag cgtctacctt gccctcaact
     4201 gcttttattc tgtcaaaaac agactttgta agctctgcgg agcttatttc cttgttcttt
     4261 atcatttctg acaagtcgca ggcatttctt tcaaaaagtt ccaattataa cactccttac
     4321 tctactgttt taggcacgac aagacagcct gcctttacct gaggagcgtt ctgcataagc
     4381 tcctgtcttg tgaatttatc ctgaacagcc acgtcttttc tgagtatcat cgggttgtca
     4441 gggtcaaggg tcatttcgtc ctgaatgtcg gggagctttt caaccattcc cacgatgttc
     4501 tgcatttctg actcaaannn nnnnnnnnnt gatcgtcttc gatgcgaagc cttgccagct
     4561 tggcaatgtg tttaatgtca atgttcattt tacattcctc ctgttttatt tatataaaaa
     4621 gatattatct tattttatca tatccttgaa ctgttcttcg gtgattattt cgataccgag
     4681 ctgctgagct ttatcaagct tgcttcctgc ttcctcacct gcaagaacat agtccgtttt
     4741 tttggacaca gagccgcttg ccttgccgcc gtatttctca atgagtgctt ttgcttcgct
     4801 tcttttcagt gtcggcaaag tgcctgtgag aacaaaagtc tttcctgcaa agcggtcgtc
     4861 tatctgtatc ttctcatact tcgtgtttat cccacattct ttgagccttt ggataagctc
     4921 tatcatatgc tcttcatgga aagcattgta aacgctttgc gccattacgc cgccaaagcc
     4981 gtctatctcg gatatctgct cagcggaagc gttcatgatg ttgtcaaggt cgccgaactt
     5041 gtcgcacaga agcttcgcag aagcctgtcc gatatttctt atgcccagac caaacaccaa
     5101 tctatccaaa gaattggact ttgacttttc tatagcacta agcagattgt tcactgattt
     5161 ttccttgaag ttgtcaagtt gtaaaagctt gttttcgctg agagtgtaaa ggtcagccac
     5221 agaggtgata agcttgttgt caagaagggt gtgaactatc tgcggaccaa gaccgtcaat
     5281 gttcattgcg ccctttgaag caaagtgaac aatgcttctg aatatctgcg caggacattc
     5341 aacgttagga caccttaccg ccgcttcttc ctcggacctc acaagctttg ttccgcatac
     5401 aggacagcac tcagggagcg tgaaaactcc gtcgccatgt tttttcacag aacccagcac
     5461 ctcaggaatg atgtctcctg ccttgcgcac cttgatgata tcccccacgg aaatgttttt
     5521 ctctcttata aaatcctgat tgtgtagggt cgcccttgaa acgctcgtgc ctgcaagaaa
     5581 aacaggctca aacacggcca caggtgttat agcacctgtt ctgccaacgt taagctctat
     5641 atcaagcagc tttgaggtct tttcctcagg cggaaacttg taagccacag cccactttgg
     5701 cacttttgca gttgcaccaa gtatctcacg ctgtctgaaa tcgtcaacct ttataacaac
     5761 gccgtcaata tcgaatggca ggtcaaaacg cttttctccg atagccttta tgcagcctat
     5821 gacctcatca gcggtagaca cacgtttgta atcagggata gtcttgaatc ccagtgtttt
     5881 cagatagtca agtgactcct tgtgagaagt aagttccttg ccctctatct gctgaacatt
     5941 gaacacaaag atatcaagct ttcttttagc cgctatctta gggtttttct gtctaagaga
     6001 acctgcggcg gcgtttcttg gatttttgaa aggctgctcg tcattatcct cctgctcttt
     6061 aacaagcttc agaaaaacat ttctcggcat atatgtttca cctctgactt ctatcaaagg
     6121 cagttcctcg tttattttta taggaatgga ctttacagtt ttcagattag aagtaacgtc
     6181 ctcgcccaca aagccgtcac cacgggtaga acctatggca agctcaccgt tatgatattc
     6241 aagggatacc gaaagtccgt ctattttagg ctcaacaacg aattgtggct tatcaacagc
     6301 ctgctgaact gtctcaataa agcttctcac ctgttcaaag gaaaaaacgt cctgcaaaga
     6361 acccatttgc acacggtgtg tgactttctc aaatattgat atcgccttgc cgcctacacg
     6421 ctgagtaggg gagtcgcttc tgataaactg cggaaactgc tcctcaagac cctttagttc
     6481 ctgctggagc atatcgtatt catagtctgt gatctcgttc ctgtcctcaa catagtaaag
     6541 ctgagagtga taattcaaaa gcttcacaag ctcgtctata cgttttttag cttcctgttc
     6601 gttcaaaata acacaactcc atttctgcca actttttacg gcatatcata cgcagggcaa
     6661 taccacataa ggattgtacg caagatgcgt agccggattt ttcgttagct tgtatagaaa
     6721 cgacgcagga atacttccgt atttcaagga gtttctgtgc gagataacgg aaaatctgca
     6781 agcagattgc gtgcaaagcc gttaggcgtg ccttatgcgg tattgccctt tatattatac
     6841 cataattgct ggtgaaatac aagccttttt tctataaact gcgaaaaaat cagccttttc
     6901 ccttgcctaa gtacatatca ggtatctccc ctacgataac agtttcgctt acgggatagt
     6961 catagctcac cacgatatcc gtagagtgtg caggaagaat ggctgtcatt tctatggtca
     7021 cagtgagata gaggctgtat tttgtctgat ttatccctgc ttccgttaaa tcgcttttaa
     7081 gctctgtttt tgccgcacct atctgatgaa gccgcagtgg tatgtcaaaa cctctgcctg
     7141 tgaggatatt tattcctgaa agtgtgccga tgggtacgct gagttttgta ccctcaagct
     7201 gtgaaaggct ttcgtttaca gcccttgaaa cactgctttg aagcctgttc acagcgttcg
     7261 tgtcgctgtt aatagatgtt atcctgccgt cagtttcgga tatctttata taactgctct
     7321 ggggcagttt ttcaagctct ctgcttatgg ctgcatttat cacgtcaagt gccgcctgct
     7381 tgctttcaaa ctcgcttatt tcgctgatgt gccttgaaag ctgtgcaaaa aacaccgtcg
     7441 tgagcagtgc aagaataata acgacagcaa gaattatgta tgaaattctg ttcttgaaag
     7501 aacgcttgtg gacgctcaaa aaaatcaccg cctttggctt attatatagc aaaatgcggt
     7561 gatatgtgat tattttactt taattcaacg atagttacgc cgtcctcgcc ctcgccgtaa
     7621 acgccttttc tgaatgattt tacagacggg tggtgtttaa gcgcacgcct tatggcgtca
     7681 cggagaatac cagtgcctct gccgtggatt atggtgagca tcgtcacccc tgacatgata
     7741 gcgtcagaaa tgaactgctc catttcgtga acgccctcgt ctgcggcata gcctctgata
     7801 tcaagctcgg tggatacctt tcttgtcatg cggctttcga tgccgctttt ggatacgccg
     7861 ccttttttca caggcttctt tgtctgcttt tgcggctgtt gtttttcaat aagctgaagc
     7921 ttctttacat nnnnnnnnnn gttttcatta tgcctgcctg aatgaagcac attccgctgt
     7981 tgtcaggggg cgttataatg ataccctttc tgtttgtgtc aacgataagc acgttgtcgc
     8041 cctttttcag cggccttggc agtacatatc cctcgtttct gctttcagaa acagggttag
     8101 cctcgctgta aagcttgttc attgtggact tctgcttgtg ccttgcgtca atggctttct
     8161 gagtgaagtt tggattttct ttctcttttc tcagcttgtc aagctcgtca acaagcgctt
     8221 gcgattcacg ctgaacacga tttacgatct gctgagcctc acgtcttgcc ttttcaagct
     8281 cggcttcttt ctgatcgtag aatttttctt tctgctctgc aagctctttt ctgagagcct
     8341 cgctttccgc acggtatttc tccgcaagcc tgttgttttc ttcaagctgt attcttgtgc
     8401 gttcaagatt gtctatgata gtttcaaact ttctgttatc ctcagaaata agcgactcag
     8461 cgtacttgat tatatccttg tcgataccaa gactttcaga gatagcaaaa gcgttggact
     8521 taccgggtga gcctatgata agcctgtatg tcgggtgcaa agtctgcacg tcaaactcgc
     8581 atgaagcatt ctcaacgtca ggagtgtcga tagcatacat tttaagctcc tgatagtgag
     8641 tagttgtaac tagcgtcgca ccgaatatcc ttatgcgttc gatgatagcc accgccagtg
     8701 cagcaccctc cacagggtca gtgcctgaac caagctcgtc aagaagcaca aggctttcgc
     8761 tgtccgcttc gtcaagtatg gtctttactc tgcttatgtg agaggagaat gtagacagat
     8821 tgttttctat agactgctgg tcgccgatat caacaagcac gtttttatat acagatatcc
     8881 tgcttccgtc tgacgcagga atgagcagac cgcacattgt cataagcgtg agcagaccca
     8941 cagttttaag cacaactgtc ttaccgcccg tgttaggacc tgttattatc agggagtgat
     9001 agtcagtgcc aatagaaaag tttataggca cgaccttttt ggggtcgata agagggtgac
     9061 gtgccttgtt caacacgata acaccgtcat tggatatctc agggagcatt gctctcattt
     9121 ttgcaccgag gtttgccttt gcaaaataaa ggttaagctc tgttaccgca ttgtagtttg
     9181 aaatgagctg gtctttcatt tcggcacatt ccatggaaaa ttcagcgatt atcctgtgta
     9241 tctcgtcctg ctcctgaccc tgcaaaattc tgatatcatt gtttgcctcc acaactgaca
     9301 taggctctat aaaaagcgtt gagcctgttg cggaagtgtc atgaacaaga ccgctgacat
     9361 tgcccttgaa ctcagttttt accggaagaa cgaaacgtcc gtcacgcatt gtgacaatgc
     9421 tctcctgcaa atatttctga gtgctcgggg attttatcat cttgtcaagg gtctctctta
     9481 tcttaagccc tgaacgtgtt atcttctgtc ttatgcttct cagctccgca cttgcgtcgt
     9541 cagaaagctt tgtgtcatct atgatagccg tttccaatct ctgctgcaga tacttgtttg
     9601 gatagagcat ttcaaagaga tatgaaaggg aagtgtcctt gttctcacac tgctcatacc
     9661 agtggtgaag ctcataggtc tgcgcaagca agcgtcttat ctggataagc tctccgagag
     9721 aaagcacacc gcctgcctcg gctcttttga tgtaagttga tacgtcattt atgttgtaga
     9781 aattcggcgt gccgtatctc acgctgagtt caagtgcttc ggcggtcttg cctgtttctt
     9841 tcttgaccgt gtaaagatca gaacaaggct ttatccccaa agccattttt ttcgtttttt
     9901 cgttggagca ttcttttgaa agcatttcaa gaattttgtt cagctccaat gaatcataaa
     9961 atctttcgtc cattatatat acctcaaaat atgacaatag gcaaagctac gcaatgnnnn
    10021 nnnnnncgca atgcctacaa aagtctgtaa aaatcaagcg acggaaactt gcctttcaga
    10081 ttatatctaa caaaccgctc tgtaaaagct gtcagttttt tctgatatct ttcgcttatc
    10141 ttgaaagcaa aaagccgctc atattccgtc agtgcaataa atcggactat gtatagcata
    10201 gtcctgtcta attcaatatc gtaaatgctg tcaggattgt aacagcattt gtcacactcc
    10261 aaaacccctg ttgacagatt gaagtgcatt atgtcagctt catacgccat gcaaaccgaa
    10321 cagccaagaa gcgcaggcct taatcctgct tcgcacagca gacgaaactc aaatatagat
    10381 ttgagaagtt cgttatccct gtcgccttta tcgagaaaat aaagtgtgtt cagcgtcagc
    10441 cgcattatct cgtcacagcc gctttcctct gttcgtatat acatgagaag ctcagaaaag
    10501 taagaggcaa gagccgtttt tttagggtcg agcctgatgt tgtaaaaaag cttctctgat
    10561 tcactgctgt tgagaaaaaa cattgattgg tctttagcgt tcttcttttc ctcaacgcac
    10621 agcttagagt atgcaaaaag ctgcgttgat gacgcatttt tcgtgctttt cctgccgcca
    10681 cgaacaaata tatctatgac tccacgctct gctgtgagaa cagtgacgga tttgctcgtt
    10741 tcgcccctgt cattttccct cagtatcagt cccataaagg tcagcattgt cagtatcctc
    10801 ttttctgtgt gcggcatatg tgagataatc ctctatcctg ccgctttcaa agaacttgtc
    10861 ccataaagtc atgatattca ccgccttact gatattatca gcttttttgg cggtcattat
    10921 aagcggaaaa atatgttgcc tttattccaa accgaaattc tttatcatgc cctcacggtt
    10981 tctccagccc tcttttacct tgacccagca ctggagattt acttttatct ggaagaaatc
    11041 ttcaaggtca gctcttgctt cctgacctat ctttctaagc atagagccgc ctcttccaat
    11101 aaggataccc ttgtgtgact ctctctcaca gtaaatagta gcggtaacgt caagtatagc
    11161 ttcaccgttt ctattttcac gctcattaag atgttcgata gtcacagcta tgccgtgagg
    11221 aacttcgtca ttaaggttat tgagtgcctt ttctcttatt atctcagcca ttatgacctt
    11281 ttctggctgg tctgtgaatt tatcatcagg gaagtagtgc ggaccctcta cagcgttctt
    11341 ttcaagatct tccataataa tatcgaggcc gtcattttca agaacgctca ctggtacgat
    11401 atctataaaa tcaaaaagct ttgaatactc tgctatcact gcgataagat cctctttgtt
    11461 ttcaagaaga tctatcttgt tcagcacaag tatcacctta gtcttgcctg tcttgaagcc
    11521 ctttacaagc tcacgctcgt tttcggatat tttctttgtg cagtcagcca taaggataat
    11581 aaggtcaacc cctgcaacag actcgttcac agcgttgagc atatgctcgg aaagcttgtt
    11641 tttagccttg tgcataccag gtgtgtccat gaaaacgaac tgcgtttctc cctttgtgag
    11701 aacacctgtt atctttgttc ttgttgtctg cggcttgttg ctcactgcgg ctatcttttc
    11761 gcccacaagt gcgtttagca atgaggattt gcctgcgttc gcacgtccaa caatagtgac
    11821 gaacgcattt tttgaatttg catttttctt gttcatatat atttttcctt atctttttta
    11881 tctttgtcag caggcgtttt gaacacccat acgagccaca gtatcacacc tgcggctaaa
    11941 gctatcccta gccatatgtt tcttgcaaaa taagaggcta tagccataaa agcataatag
    12001 tcccagaata tccttattcc cacagccaca gaagctgccg ccgcaatgag aacagcccct
    12061 gccgcacagt ctttagcctt tttagcaagg gggtttttat ctttacacgc catatccaca
    12121 gcggcttcaa cagccgtgtt cacaagctct agtgaaataa caagtcctat gcagatataa
    12181 actgcggcag tttcggcggc gctaagcccg taaaatcgca ttagcagtat cacaagcact
    12241 gcagcgccaa tgtgaaaacg catattgcgc tctgttttta tgcagctcac aacgccctca
    12301 aaggcataaa caaagccctt gaagaaacgt cctatccctg ttttacctgt gttcatgctc
    12361 gcctaagaaa gtggcgtcac gggatatgcc cagattgcca agtatagctt cttccttttc
    12421 acgcattatc ctctcctgaa gattgctttc ttcatggtca tagccaagga gatgaagcat
    12481 tgaatgtact gtcaaaaagc ctatctctct ttcaagagaa tgtccatata tctcagcctg
    12541 acggtaagca gtttcaaggc ttatgactat atctcccagc aggtaagcgc ctgtttcgtg
    12601 gttcatgtca aaatcaccgt tctcgccaag aggaaagctc agcacatctg ttgagcggtc
    12661 tatgtttcta tgctccctgt taagctcatg tatctgctcg tcattcacaa aagatacaga
    12721 aacttcaaaa tcatcattgt ggccctcata ttccaacaca gcatggcaac agcgtcttac
    12781 aagaagcctt atgccaacag gtatctttac ctctgtctgg ttgttggtta tcattacttt
    12841 taccttgccc attacttttt cacattcctt ttttgttata tttttcataa gcaaggatta
    12901 tgtcctgcac aagcttgtga cggacaacgt ccttgtcggt aaatctgttt atcgatatat
    12961 cgtcaatgcc tttgagtacg ttcattgcct gaataagtcc tgactttctc tggtcaggca
    13021 ggtctatctg cgttatatcg cctgttatga ctatctttga gttgaatcca agacgtgtaa
    13081 ggaacatctt catctgctcg tgagtggtgt tctgcgcttc atcaaggata atgaaagaat
    13141 cgtcaagagt acgtcctctc atgtatgcca gcggagcaac ttcgatacag ccacgctcct
    13201 gctgtttagc gaaagtgtca ggaccgagca tttcaaaaag tgcgtcataa agcggtctga
    13261 gatatgggtc taccttacat tgcaggtcgc ccggaagaaa gcccagcttt tctcctgctt
    13321 caaccgcagg acgtgtgagg attatcttct ctacctcgtg cgccttgaaa gcctttaccg
    13381 ccattgccac cgcaagataa gtcttgcctg ttcccgcagg accaatgccc atgacgatag
    13441 tctttttcct tattgcttca acatatcttt tctgaccgag agttttcggc ttgacgagct
    13501 ttccgctcac cgttacgcac acgccgtcat cagacatatg ctttatctca tactgcttgc
    13561 cctcacgcac aagtgtgaaa acatatctta tagtctgctc agtgatagcc tcgcccttgt
    13621 tgataagagc aataaggcta tttatagctt ccgaggcgtt aaatacgttc tcctcatcgc
    13681 ctgttatctt gatatcgtca cctctgccga gaatcacaac attatactct ttttggataa
    13741 gattaacatt ctcgtcataa ttgccgaaaa ggctgagtac agtttccatt ctgtccacgc
    13801 tgataagttt ttccgccatt agcacaactt cctctttttt tgttctgata tacctattat
    13861 acaaagctat tatatcacat ttttcctctg attgcaacaa tctcagtaag atttaacact
    13921 ttttttatgc accttttcac actttttgat tattttttaa taaaaaattc cacctctctg
    13981 caaagctcgc catatagggt gt
//
LOCUS       DAWEMR010000056        13984 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1807_length_13984_cov_8.811903, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000056 DAWEMR010000000
VERSION     DAWEMR010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13984)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13984)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13984
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1807_length_13984_cov_8.811903"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10104..10113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13703..13712
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4058 a         2809 c         2008 g         5089 t
ORIGIN      
        1 tttatattgg cgtaatagcc ctaccttatt ttcttcaaaa tgtagaaata tctcatgttg
       61 cttttgtgtg gatttaaagc tctcgtacat ttcataacaa tcgtttatgt tatctcttat
      121 tacactatcc aatactgact ctagcacata tgaatgaccg tcattgtttc caacactctc
      181 aagataatac ttgataaatg ccacaactct tttggttgat atcctgatat ctggatttaa
      241 cttcaacgcc aaatcatcga ctctcttaaa acccaaacca cgaatttctg tcatgatgta
      301 aggattgtca agtaactttt ccttcaataa ttgagggtta ggttcattag aaatcaattt
      361 acttatcatg gcatatgtta cacccaacgg ctgaagcata ataagaatat cggaaataac
      421 atagttattc aatatattgt cttttatcct attccaactc ctttcgccta tacccttgat
      481 tttcgtaaaa tcaatttctc tattatgaat aacatcatca attacatttg ggtaaacagc
      541 taaaatgttt tttgcttgca gttctgtgac ctgagttttc aaatatgcta tttgttgttc
      601 ttctgtctta ggcacatttg cagtaattga aattggtgta tactgatacg aattatattt
      661 actgttaaaa gaacaagtaa cctcagcatt gtactcgaca ccgattgtca ggcgttgcat
      721 tttacctgcc aatgtgctac cttttaactg ccttgggttg tcaccaaaag gatcgtcata
      781 acaatcataa aaatatggaa tgtcattaga agttgttgtg aatgtgtaca ctccccaatt
      841 actattttcg ttataaaatc gctcctgttg aggaacgatt ttaaacttaa atgttttttc
      901 tgtcatgtct tttcttcctt tctgaaagcc attcaacata tggtcgcata gcctgtattg
      961 taactttatc ttcgtctgtt ttcctgcatt taatagcaac ctgagagcct ttcttaacca
     1021 aatcttcata ctgtacaagt tgactattcc aaagaacacc ctctataata ccgaaagtag
     1081 agtaaatgtt cacaaaagca aatggttttt tatttctgtc ctttttcttt tgtactctgg
     1141 aaataacacc tacaataacg caatcattat cattctcaac ggcttcaaat gctgttgtta
     1201 gataggggag tgcttcttca aatggattat tgtgtataaa tatctgtaat gcttcaaact
     1261 cccaaaaatc agcgttttca agatatttgt tattggttga aagaaattgt ttcaacctat
     1321 cttcttgctg taaatcaaac ttttctttct ttttctgatt tgcaagagtg agtaacaaat
     1381 ctttgtcata gtcatacttg ccgttaccga tacgatattt ttcaatatca atatcatagt
     1441 caataataag tttgttatat gttggcaact tagacaattc tttatactct aatggtttat
     1501 acaatgactt caaatatttt aacaaacaac tctttttatc tttcgtaggt attgcacctg
     1561 acttcattaa gttaataatt tgagtttttg ccagtgttgt tcttgacagc aagtcttgaa
     1621 ggtttttata cttgccgttc ttctcacgat cagcaacaat ctcttgggct attcgttcac
     1681 caatgcctgt aatcgcagaa aaaccaaaca gcacattgtt atcgtaaata gaaaaatcga
     1741 cttgcgattt attaatatga ggtggtaaaa cagttactcc aaactgttta gagtctacaa
     1801 tgtatttatt caccatacct gccttatctt tgttcaaatt aaatagtgct ttgaaaaaat
     1861 aaacaggata atttattttt agataagcag tttgaaagca tagaacagcg tagctataac
     1921 tatgcgattt gttgaacaga tagccacctt tagttttcaa ttcttcacta atcgttttgg
     1981 caatttcatg agaatatcca ttgtcaataa tttcttggta cagtttttct gactcttgct
     2041 taacaagttc aatattcttt ttgcctatcg ccttacggaa taagtcagct ccaccatagc
     2101 ttctgccacc aaaagttctt acaatatcca aaagttgttc ctgatagatc atgcagccgt
     2161 aagtgctttc caaaataggc ttcatgtcgg ggtgtatata ggtgacaagt gaaggatcat
     2221 gcttgcattt aataaactcc tccaaagctc ccattgaatc aggtctatac aatgccaaaa
     2281 tagccgacaa atcttccatg ttagttgctt gtagtctgag cagtaagtct ttcatacctg
     2341 cactttccac ctgaaacaca ccattcgtca atgctttgtt taacagttca aatggacttc
     2401 tatcattttc aaatttgggg ttgttgatat ttatatcata ttcagataag tgcaagtcat
     2461 tttgaatttc ctgtaccatt tttaaggtct gaacaccaag aatatcaaac ttaataatgc
     2521 ctatttgctc gacaagtctt ttatcaactt gaataacgtg ttcaccgtca gagcctagtt
     2581 tcattgccat ataatcgcta atatcggtat caacaatacc gacaccgcct gcatgacagc
     2641 taaccgtttt aaccctacca cttaatttgc ctgctatgtc aagtaactca ctgtactcag
     2701 ggtgttcaga taggtagttt atgttgttgt caatacactc ttggaatgta ttgtacgaaa
     2761 actttttgga tagtttatcc ctttcattat atttgaaacc tagtatttta ccaacatctt
     2821 ttatggctac aacaggtgtt atatacgaga agtttataat ctgacaaaca cgattttcac
     2881 catatttatc aatgagataa tttattactg taggtctgtc tgaaacatca atgtccaact
     2941 ttactacatt acatttctgc aatgaataga ctatatcttc accatgcgta ttacagttct
     3001 aataatacgt ttaggtgtgt ggcacttcga gtcaagaatt tcacttgacc ctacgctcct
     3061 ttgagctagt cgtttgacgt tttgcattta tgatttaata agtgttattc cctttgattt
     3121 ataatttctt acaatacccg ataaactagc accgtaatgt tcatttgcgt atttagctgc
     3181 gtctaaaata gattgaaaat gccctagaaa tacattgtct ttatataaat cgcaagtcac
     3241 ataatttctt atttgcgaca ttgtgcgata actgtaatgt atgttttctt tagcggtaca
     3301 ccattctaag ttatcaacat tattattttt agtattgcaa tctaaatgat taatctgtgg
     3361 taaatgattg tagttaggaa gaaacgcctg agcaactatt ctatgtacta ataatttatg
     3421 gaatttatta tctatttcat tctttaattg taccattaaa tatttacctt gcgagtcagc
     3481 ccacggcttt attcttttta gtttgcccga ctttgaggag taaatttctc ccaactcatt
     3541 tacataataa tctttatagt tttcaatttc ttttagcata ttgtctcctt tgttttgcaa
     3601 cgggaaacaa tattaaatca taaaatgcaa cttcgcacag gattgtcata tcgtcagaca
     3661 gaacgactta gatgttccct gttagctaat taacacaccg ccatttcctg cggttacaat
     3721 tataataatt gtttaattaa caccctatat tttataggtt caccacactt aacacatatg
     3781 gtttcccata tgctcgaccg aaaatcaatc tggcattgaa actctctcag gattgaggaa
     3841 caatttgtta ttaaccatag gctctttatc ctatgctctg gaggtttccc tcattttcat
     3901 ctgttggtta cttccaaccc agtttagact atatttttca aacttcattc atttatttaa
     3961 agtttttatt ccgtcttcgt gggaaattag tggctctaaa gtctcatttc ctagtcgtta
     4021 cacactttct tttatcacta aaagatttgg ctcggtattc cctttatctc acctagttat
     4081 aggtttaggg tttcttagtc agcttattcg tctatggtct tgtctcatta tcggtttgct
     4141 ctcaatgaga agtcttagtt tgctgatacc gaattaacgg aatttaacga gtgcaaccta
     4201 tctacgctca aaaatcaatc catatttaat agggttaaga tcagttatac ctatcgtata
     4261 acacacaagg cttcctgctc cagagccacg tcccgaacct attttaaccc catgagtttt
     4321 cgaataatta ataaaatccc atacaataat gaaataaccg tcaaaattca tttgatgaat
     4381 aattcccatt tcataatcaa gtcggtcttt aattatcttc tgttcttctt tagaaagctt
     4441 gtcaaaattt ctagttttcc acccctcatt aataaggtgt aaaaggaatt cattattaga
     4501 cttatatcca cttggtaagg ggtatgtcgg taactgtggg tcttgaaaag gcatatgtac
     4561 ttcttctatc atatcagcta aagcattagt ctgatttaaa ccttttgtaa cattatttac
     4621 cccaatttgt ttatccatag ttgtatgaat ttcttcttca ctttgcagat aacagccttc
     4681 ataactttca gacattgttt cagtgtcgtc tgaacgtgcc tgccctgata atataaatct
     4741 tcctttgtgg ctgcgtggct atctgtagta attatgtatg gagtgtttgt tacctcagat
     4801 agtttcaaaa tctttttatt gtaattagcc tgctcctctg atttgtgaga ttgcatttcc
     4861 aaatagaaat taggaaatgc cgatttgtat tcttcgatat acttaacaca aatattaaaa
     4921 tcactttctt tagctaattt tgaagccaaa caagcagaac aaataattaa atcttctgca
     4981 tacggagcaa tatctgaaat ctgtactcta ggcttaaaat aaaaattttc aagatttgac
     5041 ttagtgataa ttttatttaa agcctttctg ccgttctcat tttttgcgag agcgataaga
     5101 tggaaatact tattgttttt atcttttatg gcagtatcga agcactcata cagctctacg
     5161 ccatatatca gcttaatatc aggatattct ttagatagtt gatcgaaata tatccatgaa
     5221 tattggttgc catgttccgt aactgcatat gctttaatgc cgacttttcg acattgctct
     5281 agcatttctt ttggtgtacc atagccgtcc agtaacgagt acattgtatg gtcatgcaaa
     5341 gaactataca ttttcagcct ccttgtattt taaaataact atctgagggg taattacacc
     5401 cttatactca gatacattga gctggcagag tgcattaatg cacatttcat catcatatcc
     5461 attcaaaaag tctaatactt tatcgttact aggattacag aacttgataa ttgcgatatt
     5521 atcgtcagta ataaacttcc atgtatcttc atttttaccc atgataacgc cttggctatg
     5581 ctccaaaact atattattaa tgacaaataa aggctctttg attcctgtac cgtaacaatt
     5641 ctccaatgat gtaacatcgg aaatcattcc aatattaaat tcgtcataat cgaaacaaaa
     5701 atctattggc aaaggattgt ctgaatcaat attcttattt aaaactttaa ttgcttcagc
     5761 cacgttctca gcttttatct caaaaccgaa agcatttgcg tgaccctgac accaattaaa
     5821 cagacctgtt tttagtaact cagcctttaa atctggcaca tagctattat caaagtttct
     5881 agcagaccct ctatatacat tattttcttc atctttgcgg agtatcaaac aaggtttttt
     5941 cgcataacta gccattttca tggctatcaa tccagaaaat acacttggga tattgttacc
     6001 ttttaagaat aaaactgtat tttcgtcatt agctacgctt ttccttaacg caggaagtaa
     6061 ctttttcact tgattatcct gtcttgattt agcgttttta cagaatctta caactctctg
     6121 ataaatattt tcttttgtac tttcagtttc accacgtttt ttgtattcaa attcttcgtc
     6181 ctgctcaata aacgctctga aaagcaagtt cttttcttcc acatcaccga ctctacacat
     6241 tgcatttatc agggaagtaa tgcaaaatgc aatagtatga ggattaacct tgcctttcat
     6301 ggaataattt tgagcattaa taaatccttc aaagcattta tttgtgacgt tataaagacc
     6361 tttatcaata agcctttttg tttcaaaaga acgtaaatcc ataatgtcag aaatattagc
     6421 caatgcaaca aggtcaaggt agtcatcggc atagtcgttc caataataat cgtcaagtgc
     6481 ttgtagaaat ttatagacca ctcctgcacc acataattct ttattagaat attttgaact
     6541 agattggtta tttactataa ccgcatatgg atttgttctt tcaacatcat ggtgatcgag
     6601 aacaagtaca tcaacacctt gttctgtcaa ctgtttgcat tgttcagtat cattacttcc
     6661 tgcatcggga ataatcaaca attttgtgct ttcaggtatt tctatctcag aagaaatacc
     6721 atgttgcttt ccagaatgta tcagatatgt aatatcaatt tctttgttaa gccgtttcag
     6781 ataagaatat attatggcag cactgcattg accgtcaaca tcgcaatcaa caataatcgc
     6841 cattttacta ttacttttaa tgtgtctatc tagcatttga accgcttcat taatatcatc
     6901 aagattatca taagaaatta atacgtcatc agttaaatga gtgtattcat taacattagt
     6961 tattcctcta ttaataaaaa tagatattcc cattctttat aacttgcgta tatttggcaa
     7021 ttaactgttt aaacttatcg ggattatctg ttggactttc tttttcttcc agtaaatttt
     7081 cagtatcaac aatagcactg atttgaatac aatccagaaa tttgtccgct atatcgttta
     7141 actcgtctat ggttacatct ttatcaaagc aaaatataat atgagaactc agtcttgtca
     7201 gcatatttat ttgatattgg cttattttct taccgcaagt tgctacgcaa ttttttattc
     7261 ccatgttcca aagttgcata acaccttttt cagcttcaac cacataaacg tagcctgtcc
     7321 gagctatata tttttcggat aaataaagtc catataatag tctagctctg ttacaacgct
     7381 ccaaatatat atacttaact ctttgttctt cttctgtcat ttcttcttgc tttaaaaata
     7441 gtctaccctt aacaccgacc aatgtcccca tttcatctct tacaggaatt gtaattcgat
     7501 tggaaacatc gtcataacct atttcaaaca gcatttgagt atcatatgag atattatctt
     7561 tcaagaaata atcattaacg gcagggaagt agtacgatag aacattttcc ttaattggct
     7621 ttaaaggttg catttcttcg taattagact catcatctgc catttcagat ataaattttg
     7681 tgaactttag actttcgggc aaatcgttat attcgtcttt ataatagtca ataccgcacc
     7741 aattacaaac tttgcgaaca gcttcgtaaa acgtacaact gcaaaaaaaa cgcacaaggt
     7801 caaaaatatc tattgtatct aagtttgaac tactatgtat ttctcgtgtg tagtcaacag
     7861 ttaaaagacc ttcattgaga taaacagtga tcgccccttg attatcgcca tcaggattgc
     7921 cacactgaac ataacctgct ttgcaggaaa tatggtgaca acctatttca tctagtatga
     7981 caggaacata attgttctct agtatctttt ctttgaggac agaaatatcc attttatcct
     8041 cactttcttc ttagttctcc gacttcatac caagtgttta gatccaagtc aacttcaaat
     8101 acaactttct ttttacaacc aaatctattt ttgtctacat tgcccacata atacctcttg
     8161 ccaactttaa gttcgcattc aacatctttg ccccattcgg catcatgctg aacatagcga
     8221 tatttatgaa agtcaccaac agaaatttct ttaaacagtg tcatcgtcca aataatatgc
     8281 tttagctgtt ttgcattagc aatattattt gagtttagtt cgtcaggctt acaaaactcc
     8341 gtatcgtctg tgagctgaat tgagagataa ccaaacatat ttagttgctt tgctaaatca
     8401 gtgagttttg ttactgttgc ttttaaagct gcccaatctc ctgtggcttg tgtgtcttgc
     8461 ttgcaggtat cgtaaaagaa gtatttcgca ccatgagtta gattagcttt tcttatttca
     8521 aactctagtg ttttgtcatc ataaccgcca gccatgtcct taacgagaat aagttcatta
     8581 gtttctgttt caatccattc agcaattttc attattttta catattcctc tgaattgtca
     8641 gcgacccttt gaatgtactc ttgcaaagtt tctgttggct ctccccaatc gtctgttttc
     8701 tgatatatgt attcacctga tttatccttg tacaaaccaa gtgttaattc cttttcaggc
     8761 ttctttaatt tgatgccgtg taacttttga aactcaacat tgtttataca cgttgtaatt
     8821 aaacactttc tgagatcgtc cacacccatt tcattaagca taacaaagac tctttcatgc
     8881 tttacaagcg ttaaataggc aattattttt gtcataaatc gtgattttcc tgcattggaa
     8941 agcataccaa tagccattgt cgagcctagt ttacaacctc taaatatgtc atttagaata
     9001 ggaaagggaa gtgatacacc caaatcaggc ttctccatac acgcaataag cgattgttta
     9061 atgtggctat tcagaatttc ggcttcttgg tttgtcaaga ttattgtatg tattctatct
     9121 gctttacctc taattaatct gtagatgtct gaagccgtaa attgttcaaa cttcttatgc
     9181 tctacaattt ttgtaatatc aaagccattt ctttgatatt ctcttaacaa agaatacttt
     9241 ttaatgattt cctgatactt gccaatgtca tcagttatag caattttcat ccaactgtca
     9301 agagttttcc aaccgccata ctttttatac aaagaaagtc tctcaggctc ttctgaaaaa
     9361 taagttaaaa tagtagtttt attgaatgtt tgtgtcctag ttttgtatat gatttcggct
     9421 gagtcataaa aaaatcgtgt aacttcatct gaaaaatcat atttactacg gatatattgt
     9481 ccgtaattta ccagcaaatc aggctgtttg taaatacaac ccacaaatag aacttcggta
     9541 ggaacgtttg ttataatatc catgtttgtc acctacctaa atttcattaa taatactgtc
     9601 aatatcaagg ctatcattgt ttttatcacg ttctttagga gattttgatg ttgccatttt
     9661 ttcataatct atattaactt gttcttcact tgtacctgtt ttagccaatg tctgttcttc
     9721 tttccatttc aaataaccat catatttaga taggataata gcgagatcat atgtaattaa
     9781 cgctgcacct tcaatttttt tacctttacg agtattaaac tcatgtacct tgcgaagaaa
     9841 tgacattttc tttcgccaca tatcccataa atcttcaaca ggaacaggtt tgttcaaatt
     9901 cttataagtg cctttataca ccttatcaag atttataaaa aaatattttg gcaagaatga
     9961 aatatcatat tgtttgtaca accaatctgt aaactgtgtt cttgtttttt tgtcctgctt
    10021 gtctttctct atctgttctt ttgttcttct ttttgccaag tatttcaccg ccttaatcaa
    10081 aataactaaa tgaagcaagt gagnnnnnnn nnnaagtgag ggaataatcc tcacttgttt
    10141 catttataaa aattaaatct tagaaataac ttcaagaacc ctttcaagag ttttgacatc
    10201 ggtgatcttc ttcatttctg ttggtttaat aggcagatct tctgctgaaa gagcttcctt
    10261 tgcctttgtc ttaccgacag gattaagact tttcataacg gctgaaatct tatcaagaag
    10321 ttctgttgtc tgattttcgg cagagttttc atcaatacta tcaactggct ctccaacctt
    10381 acccataact tcctttgtat aaatatcctg ctcaatatcg acagcctttg tgagatcatt
    10441 cttaacagaa aactctttct tgtcctttgt tctgtcaata attacctgcc aatcaacaag
    10501 tgataaatct tcaactgttt ccttatcgtg tacacctgtc ctgtccttgc tgatgtacga
    10561 acagaaattg ttatcctcgt taatgtacat tcttacaaca gttttaacgt tgtagttcat
    10621 ctgcttaaag ccgtcaggaa tttttctgcc tgttgcaacg ctggtaattt taccatcgtc
    10681 accctttacg gaaacctttt cgtccgtttc tctggcggtc acaataaagt gcgctccgca
    10741 ggacatgaga tcaagtatca aatcctgtcc cttaaaatta actgtctgat aatctttgag
    10801 ttcaagtcct gcaccctcga tcgttacagt tttttcaatg ccagttagtt cctttttctt
    10861 tgccttaaca gtgtttctct tcttggagaa ctccacaagt gcctgcttag ttgtcaggtt
    10921 aagaatagtt gtaccatcaa ctacaatagc atcagctctg aatggctcac catctccgtc
    10981 aagtataatc tcgtctgttt cattgccatc gtcatcgaga acatgaaaat cttccttgtt
    11041 cttaacttta tttatgtatt ctcttgtttc accgagagac tgcgtgtata cgatataaat
    11101 attctcagtg ttaatgccgt cagcttcaag cccaccgata aaatcatcaa tagagccgtt
    11161 ctcattatct atgtaaagaa ctctaaatgg cttgccgtca ggtcttttaa aataagcaag
    11221 ctgcaaggcg agtgttgact tgcctgtgcc ttcttctcca aaaagtatca tctgaagctt
    11281 gctttgtgtc tgtgttgctt ttcttgctct agccatattt tttatctcct tttatattat
    11341 cgtttcgttg ttaataatag tgagtagtaa caatttacca ctcatcgtcc tcgtctgtca
    11401 gatcattatc tgaaacagaa ccccaatcat tatcatcaga gccaaagtcc ttatttgcgt
    11461 tttcggtagc ctttgtcttt gcgatagcct tatcaataat ttcttctgaa taaagttctg
    11521 tatctacgct atccttatca gctccagtaa tgagaagtat tctctttgtc ggattgttca
    11581 ctctatccat agggttgctt tcgccccaac cgtcatcatc atcttcctca atttcttcaa
    11641 tatcatgttc tatcataata tcaccaaaga ccttgagggc tgtatatggc ttgagctttc
    11701 ttagagtgct tgcaaacttt gatttactct tgtcaataac gaactccgca tcttctatag
    11761 aattgtaagt tacaatcttt gcagatacag tgaagttacc ctcgtcattc ttttcaatgc
    11821 ccatgaacac aataacctgc tcgaaattgc caatgacatt aaagtcctct gcgtcaaaat
    11881 ctacgtcctt gcagagtgaa atctgtgacg gaacaaatct agtctggtgt ctatcctgat
    11941 aggtggaaaa ctcattcttt cctctgacaa acacggacat accgtccttt gcattatctg
    12001 ctatgtactt acaagcatca tattcaacaa gtatcttctt gtcgtttact tccttacctg
    12061 tcgagtcagt cacctttgtc aagccgagat taattccaat aggtctaaag tcctttttgt
    12121 taaatgtaaa tctgtcagcc cactttacct tttctgttgt tgtctttcta tccttacctt
    12181 tgtcttcggt cttagagaaa tatactacat ctctttccat accattgaga tttatgtata
    12241 cagacttatt cttgtcaatt tcaactccta cattaaccat tctcattggt ttgcctgtag
    12301 aggttgtcag ttctgtatag aacttgtcct tatcacagcc tgtcagctta cctctgatct
    12361 gaaaactgcc ctttgtttcc tgaagtccaa gacccttatt atttttcttt tcagccattt
    12421 tatttctcct tttatgtatt tatcagattt tgttgtcaaa taaaattatc attttgtgaa
    12481 ccaaaatcac accatcttat catgccttct ttctaaaaca cattaaattt aatttatcta
    12541 acgttaatga tttctattgc tactaacatt tccctcacgt cctcttcatc acaacaatca
    12601 aagaaaaggt cattgccatt atcatcgtgc aatctacaag aaaagctctc gttatcttcg
    12661 tcaacctcaa attcgcagtt gcttgaaacg atatcaacac tacacattgt agcaaaaata
    12721 tctgggtcaa gaagatcagc tcctcgacaa gtgccaccaa cctcagtagt aaaccaaccc
    12781 ttatacttac cgtgttgtaa tgtatattta atctcgtgcc aatttctgcc gtcctttggg
    12841 ttatatgttt ccattacttg ccctccttca tattttcaag ttcttcatgc aacgcagtgc
    12901 cgaaattatt cagtgactct gctacccatg tatcagcaat gtcatatcta ctaattaaat
    12961 tgtatattgc tttatttata tcagagtgcg agaactgctt atcacatcta tactcagatt
    13021 tttctttagg gtttatttta gtattaaaaa aacgtatctc tttattatca caactagcgt
    13081 taggaaaata tattctagcc aaggcaagca aagcaccaat atatgcacta tatgtatcat
    13141 cagaacaaca ttttgaagtg ccaactctta ctaccttgcc gtattctttc attttcgcaa
    13201 ccgttgtctt atcgtggaaa gtaatctgaa tttcacggtc aatatcggac gatattttct
    13261 ttaaacagtt agcaaaacta ctataaatat aaaacatact atcgccacca tttggcttaa
    13321 ctgtttgata cctaaccatt ttattattgt atatatactc tattgcttta atccttatta
    13381 tgtttccagt ttcggtcatt ctattgccga aactatctaa accaactcga taaagttctc
    13441 caatcttaaa ttttcttttg ttcatgctta tcaaactcct ttatttatat caatacctgt
    13501 aatttcttta aatatttccg catcaaaatt tggaagtgat ttgatgatat tcttttcagg
    13561 gtctgacaag ctatcccacc aaagttgatt acattcagat ttatcacatt ttttcaaata
    13621 gccacctgtt atctcatatt caggatgctg ttctttttct tcatcagtca tctcataaga
    13681 ataaatccat ttaagaacat tgnnnnnnnn nnatgttatc tagtagtctt tttgcttcag
    13741 aataacgcca atcttcaata ctccaatcag aaggcttatt gaacattaaa atttttgatt
    13801 ctttggtatt aaagcaacca tttgaaaagt tagttttgtt aaaatctcca ctgttacaat
    13861 caccagtgtt acagttacca cagttgtagt agccgctgtt ccaattacca ctattccaat
    13921 gaccgctgtt ataactacca ccgttgcagt taccactgtt gtaatcacca gtattgtaac
    13981 cacc
//
LOCUS       DAWEMR010000057        13949 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1817_length_13949_cov_11.289405,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000057 DAWEMR010000000
VERSION     DAWEMR010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13949)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13949)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13949
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1817_length_13949_cov_11.289405"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4180 a         2786 c         2706 g         4277 t
ORIGIN      
        1 atcgagtgtg gtcatctacc ttgcgaggca gaagcacaag tcatgttaag ctgatctttc
       61 tgctgatatt atatcattta tttgtcagat agtcaatcat tcaacaattc attaaaacat
      121 taatcctatc atcttttgta tgctttgcat atattagggg gtatgttttc tacgtcccaa
      181 atcttaactg tccaataatt tacaccacaa atttctgaga atgtgatata atatagataa
      241 agcaaaatca ggatggaggt acaatatgaa ctttgataat cacaataaca atgaggatat
      301 gtttgaagat tatatggtgc ataagatgtc tacagaagat acaccctcct catctgtcag
      361 acacgattat tccggcgcaa agaaaaagag tggctcaact tttggagcag ttataggagc
      421 gatactattt ctgctgttat taagttttat acttgaagca tttgagccca gatgtgcaga
      481 acccggctgc agcaacaagg ttagcagcga aggcgattac tgcaccatac ataggatgcg
      541 gcacagctta tgtttcaggt gatctccgag tgctatgagc gcaagagcct cataataacc
      601 tccaatctgg agttctcagg ctggaacacg gtgcttggcg acaaccgcct gacggcgact
      661 ctgatagacc gactggtgca tcattcgtat attgtgatat tctcaggaga aagctaccgc
      721 ctgtagcagt cgatgaagcg tcagaaaaca gcccgtaaga agtgagcaaa gtacctgaat
      781 ttacaaaaag ggctccaaaa atgcagaaca ggagctcgct aaagggcttc aaaaatacac
      841 aacaggggcg ctccatctat gcgcaaaccg agcccccaca cgttaatctt gtgctgattt
      901 ggctatgcac tttttcgagc cccttttatg cactttctgc ttgacaaaca cacggtaaga
      961 gaacggaata tatcggggct tatgaatgaa tataaaggga gagatactat gaaaaacagc
     1021 gttaaaagaa caacagcagc tatactggcg acaatgatga taggcacagg aatgtatgca
     1081 cctatcgccg atcacggagc ttgaccttac agaagaatac gagacatata tgaaaggaaa
     1141 tgataaaaat gttagcactg aaaacaggtg acagcttttt tgagatagat ttttcacgca
     1201 aatgttatgc ggcaaatcgt tcatcggtca cgctgtataa actctgcttt gagagcagtt
     1261 atatcaatct cggcaaggtc tttgagggaa caataatgga gtgcaagcag tttgtgtatg
     1321 ataatatcgc acactttgac aaccctgctg aacactattt tggcaattat cctgcacttg
     1381 tgcagcagtg cgggagtaag aacagctctg tgttgtaaat gtaaaaagcc taattaacat
     1441 tgtcgaagtc aagtcgaatt tttagtttgg taacagaact aaaatacgca ttacagtctg
     1501 atatgtactg tcttatagag taaaacatat cgagactgaa aagaacgaga tcgaaaagct
     1561 tagaaatgtg ggaacagaat tgcataaaaa gatgcttgaa aagggttgac aaaagcattg
     1621 taatatgata taatataatt gttgaaagat aacgcacccc gttcagcgtt gaataaatgt
     1681 acggcatatg ataaattgca gcgagtctgc tgcgttcgga cataagtcac ataaggcagc
     1741 tatccttttg gcttcccccg acgaaataga cctttatagc cttgccactt tcggcaaggc
     1801 tatttttata aggataagta gcataattat gaaaaagagt gagattgttg aaggttgttg
     1861 ctataagctt gatccagtta ttgaaaatct tgattttata aagaaatcga ctcaggacat
     1921 cttttgaaaa acaaagggcg accatatatg gttgtatttt gtaataaaga agatagcact
     1981 atttgggtag caccgctatt gtgtttgtca agcagaaagt gcataaaagg ggctcgaaaa
     2041 agtgcatagc ccagtcagcc tgacccgatc gtcatttcct tggaggttct atgccacata
     2101 acctgtacaa actcgcagag cttaactgtt gttcaaattc atcgttcgta aattccggtg
     2161 aggtcatagc ggaatggtaa agtttccctg atttagccaa aagtgttcgc tgctcgacga
     2221 acctttgaaa gataaaagaa gcctatttta aggcgtattt acgttgttta cccaccatac
     2281 gaaagtgtgt tactatttgg caagacgaca acgctttctg tctgccgtca tatgtttcag
     2341 gagtatcagg tcaaagcctg cgacgctgtt gcaaacgctt tgaaattcaa tagcggctca
     2401 taacagcaaa aacgaagtcg gagcttcgga tttacatact tattttgcag aaaatacttc
     2461 gattttgcgg cgtttttgag caatgcaatt gtagtgattt taagccgatt tcggtgattt
     2521 ttagcacaaa taagtggcaa acaagtggtt gaaagtggcg catttctacc aggaagagta
     2581 ccgtatgcgt aatgacagca ggcggtcatt tcctagaagc ggtttatcga gtgtggtcat
     2641 ctaccttgcg aggcagaagc acaagtcatg ttaagctgat attatatcat ttatttgtca
     2701 gatagtcaat cattcaacaa ttcatgaaaa cattaatcct atcatctttt gtatgctttg
     2761 catatattag ggggtatgtt ttctacgtcc caaatcttaa ctgtccaata atttacgcca
     2821 caaatttctg agaatgtgat atttgagcag taacttcctg tacaattctg aacaatactg
     2881 tatagtcctg tacaatcccg aaaaatcgtc acttttcttg gtagaagcca aaaaagtccg
     2941 aaatcacgca aaactgaaaa aatctgaaaa tcatcaaaac ttggtagaaa atcggtagac
     3001 aaaccattct accaaagttg gtagaaaacc aaaaaagttg gtagaaagcc tgttgacaag
     3061 gatagcggat agtgtttcgt atcgaagcgc tgtccgctat ttttttgccc aaaattgaat
     3121 atgtgtacaa cgcttctcag caatgaggag cgtttttctt tttcagaatg aatgtcaagg
     3181 aggtctttct atgcccaacg cattacccga agcaagagca tttgatgtgc ctgatttttt
     3241 caggtttcag aaaaaggagc aacgagatgt tctcgatgct atgctcaaca atgaaaacaa
     3301 tgactactct actgtcgatg agctggcgaa gtctgaccgg ctgtcgcagt attcctatga
     3361 ggtgatcggt gatgccgtcg agtatctgac aggcagaggt tttctccata aggtcgagaa
     3421 gccttacggg tgtgtctatg cggtaaacaa gctgaggata ccccatatga tgttcaacta
     3481 tactttgtaa aggtcagagg tgagaaaatg ggcgcctccc ccagaaattc attcagttcc
     3541 tccagctgcc gatttttctt ttcaagctct tttatccgct tgtttgcttc cttcagctgc
     3601 tgcgcaaggc taagggattc ttcgggtgta cgagtgccgg cacctgtgtc tatttcaccg
     3661 gctttggctt tgcttaccca tgtcccgata gttcctttgg gtatgccaag ctcctcggct
     3721 gccgccttat ttcctatctc ttttgcaagc ttcaccgctt cttttttgta ttcctcactg
     3781 tactgttttc ctgttcccat tctgaacaca acctttcttc ttttattata tttcggtttt
     3841 gctggttgtg tcaagtttta ttatactaca tcacttcctt gtcgcagtta gagcgtgaac
     3901 agctcaaact tcgtcagtgt atataccgct cccccagcca cagctgcacg gacattctgc
     3961 cacacctcgt cgctgactgc ccacggcact gcacggaaaa tatgatatac ttgatatggc
     4021 acgtcaaaga cgtgactaca tatcaaggag gtctatcata tggtggacta caaagagtta
     4081 ctcaggctga ccggtatggg atacagcttg agacagaccg cagcaagtgt gggacattct
     4141 catcatactg tgaagaatgt tctggagctt gcagaaaagc acggagtaga atggcctatc
     4201 gatgaggatg tgactaatgc agaactcgaa aagctgtttt atccggaaag aagaacagta
     4261 ggcagacatt atgccaagcc tgactacgcc tacatccaca aggagttgtc caagaagggc
     4321 gtgacactca ctctgctttg gcaggagtac tgcgagcgtg cacacgcaaa cggcgaaacg
     4381 ccctacatga gcacgcagtt tggtgacaaa taccgccgct gggcgcgtgt tactaaggcg
     4441 actatgcgtg tgactcacaa gccgggtgat acaatgcagg tcgattgggc tggcggaact
     4501 atcccttact acgactctgt taccggtgag gagtacaaag catatctttt cgtggctgcg
     4561 ttaccttgca gcagctatct ctatgtcgaa gcctgcactg atatgaagca ggagaattgg
     4621 cttatgtgcc acgttcatgc ctatgagtat ttcggctgag tgacaagagt tctcgtcccg
     4681 gataacctca agacaggcgt aactgcaaac acgcgctatg aaacacagct taacgaaagc
     4741 taccgtgagc ttgcagagta ttacggaact gcaatcgttc ctgctcgtgt aagaaaaccg
     4801 caggacaaag ggcttgtaga acgttctgtt ggcttttcga caacgtggat aactgccgct
     4861 cttcgtgagc gcaaattctt ctcgtttgca gaggtcaaag aagctgttgc cgaacggctt
     4921 gaagtcatca acacaaagcc tttccagaag cgtcccggaa gccgcagaga ggcatatctt
     4981 tctgaggaga aggagttcat gcttccgcta ccgaaacagc cgtatgtgcc ttctgtgtgg
     5041 aaacagcaga ctgtcggtaa tgactatctt atctcagacg gaatgaacaa atactctgtg
     5101 ccgtttgacc tcatcggcga gcaagtacag atcaggctca ctaaagactt ggtggaaata
     5161 tatttcaaag gcagccgtat gacttcacat aagcgtcttg aaaaatacag cgttcaacct
     5221 gttgtgaaac ccgagcatat gcctgcaaat caccgtgagt atctgagttg caatgctgat
     5281 gaattcaaaa aatgggcggc tgccatagga aagtctaccg aagaaatcat aaagtatttt
     5341 ctgtcatccg gaaacgtacc tgaacagggc tacaaggcat gcgcaagcct tacaaagctc
     5401 ggaaaacgtt acggaaagaa gaaactggaa gccgtttgcg agcgtatgct ggcgttctct
     5461 tcatcgcctt ccatacgcac gataacaact ctcttgaaga acattaaaga acctgaaaag
     5521 catatggaaa aagccgaaaa cagcaacaac tacggcatta cccgaggcgc tgcctactgg
     5581 aagaagggcg gtgacggtaa atgatcagca atacgataga cgtctgagag ctatgaaaat
     5641 gaatgctctt gccactgaat tggagcgaca gttagaagat cccgaaagct acaagcagct
     5701 tggttttgaa gacagattaa cactcttggt cgacgctgaa tggaatcgtc gacagaacaa
     5761 caagctcaca cgatacatca gaaacgcaca cttttctgat gccaatgcaa ctattgaggg
     5821 cattgaatac atcgaggaca ggcatctcga caagggaaaa atgctgcgct ttgcaacctg
     5881 caattatgtt gatgagggca gacatattat cctcaagggt gcttccggaa atggcaaaac
     5941 atacattgcc tgcgctctcg gaaatgctgc ctgccgaaaa ttcaagaccg ttcgttacat
     6001 cagacttccg gacttgcttg atgagcttac gattgcaaga gctaacaatg agttcagcaa
     6061 ggttatcaag gcgtacaaga aggtcgatct gcttatcctt gatgaatggc tgatacgcaa
     6121 actgacaccg aatgatgctt tcaacttact ggagataatc gaagcaagga tcgaaaagtc
     6181 aatgattttc tgcacgcagt atcattcaga gggctggtat gagcgtatca attccgaccc
     6241 tgataatgac agcccgatat ccgatgcgat aattgaccga attgtcaaca atgcctacga
     6301 cataatgatc gacggcgaga tctccatgag aaaacgtcat ggtctgccgg aaggagctga
     6361 gccgtgatgg ataactattg tgctttcagt gaagatcata agtgcctgaa attggaggat
     6421 tatgagctta cccgtcacga actggaggag gctgataatc tttgtcatgg caactggata
     6481 gaaattcaga ggctgtatga ttacatcgat gaactcaaaa agattcttga tgagaacgat
     6541 attgaatatc cgggaatctg attagattca acagggagcc tgcctggttt caggcgggct
     6601 ctttttatgc cgttcagtgc cgtgggcagt cagcgacgag gtgtggcaga atgtccgtgc
     6661 agctgtggct gggggtgcgg tatatacagt cagcgtgagg gcatcgagat cgcaaaggca
     6721 cagggcaaat acacaggcag aaagcctatc cctatcgact gggcgaaatt tgagggcttg
     6781 tatggggaat ggagagcaaa gcacatcaca ggcagggatt ttatgcggag aatgggtatc
     6841 acagctaaca ccttttaccg ccgtgtgcgt gagtatgagg tcagccacgg catcaccgag
     6901 cagacttccg cctgacaccc tctcagcgga acggaaacgc tcacagaacc gtccgagcct
     6961 tgcggagagg aaaactaacc actaaaaata tcaggctctc agaaacgatt atagagccgt
     7021 ttctgagagc ctgcatatta cttgtatgtc attagggttt tcgctgattg tggtatgctc
     7081 cataattccg attttgactt gtgcggaacg gaattaaagt gcgccattag gctttggtct
     7141 aaagaaatag cttttattct tcgtatggtt taggaagtat cgaccaagct aatactcgaa
     7201 tatcagactt tgtattccat tgtttataag aagttgaata tgatagaatc tgaacctctt
     7261 tatcgcccca cttgccctga aatatatccc actcttgaca ggtaactaag caagcaattt
     7321 catctttttc tccgtttgga agttctttat cgcatggaat ccactttgtt gaatcgcttt
     7381 tattcataga aatcacctaa tcatcaaatc tatttgcaaa ctcaatatca agttccttga
     7441 ctctttctaa gcaatcgtta tatcgcttaa tagctatatc cataagttgt tttctgtttt
     7501 tactatccct tttagaactc ggatcataga taacaacaaa ttcatctatt gttgctgcaa
     7561 gagatgaata cccctctgac catttatgca aatctttgct tggtatagaa aaatctgatt
     7621 gctctaaaaa cagttccctt atttttgtca acaatggttt gatactatta gtaaaagtat
     7681 cctctgttat tgttctattg atatattctc tgtagtatcc taaaaacttt tcagctgata
     7741 gaatcatttc atacatacaa tttcgtgtag cctcagttag ttctacatca tcagctgtat
     7801 tatcagaaat aaattttctc ataaatgaat agttactata ttcttctatc catacatcat
     7861 taataacaga agatttatct gatagacgat accaatgatc tttatcttgc tggctatcaa
     7921 cccatgtact tctgaaagca taggtatgat tgatcatatc tgtttcgctg tagccataat
     7981 acgtaaagat tatattgtta ggtttgcctt tccacctttc ctcgcttatc ttgttatcat
     8041 atacttcata agagagagcg tcttttttga tttcctcagc aatatcggaa atatcttctt
     8101 tggaaaaggg aaatcttagt atgacttcta aagagattct ctttacgttg gcgtgaacag
     8161 gcttgacaga gtatatgatg tacctttctg tttctttgta tattttttgt gttgtgtaag
     8221 gtttatactc ctcgacttca aaagaaattg tacctacaat acttgaacga aacgcattat
     8281 gttgtttatt cataattgat ttgttagaaa tatcagcaag tctatcaacg tattgcttaa
     8341 tttcttcaag gctctttttg ccatttctta tcaaatatgg attggttaat gcatcataga
     8401 gaatacacgt atagcggctc atatttctac tatagttgaa tcccgatgtt tcatcaatag
     8461 cacatgatgc aaatattgca caaccatgac ctacaaattg atccgccgtt gcttgcaaat
     8521 ttaatgggga ttcaagggtg acgtttttat tcccagagtg acaagcatca ataattacga
     8581 ttttgttttt acaaggtaat ctatcgatta aagatattac ctcattcgtt gaaacaggtt
     8641 tattacttaa tacaaggtaa ttttcgctat taagttctgc accatgacca gaaaaataga
     8701 aaataaacgt gtcagaagta tcaacgctat ttattacgcc attaaacgtg ctgatgaagt
     8761 catcaatatt aactacctta ttctcaccaa gtacaagaac attgctttct tttactgcta
     8821 aacctgaaat aagagctttt ttcatagctt gaatatcatt aaggcaaagt ggtaaatttt
     8881 tacatcctgc tccaagtgta tattcactaa caccaacaat caaggcttta atcattcttt
     8941 tctcctaact aattacagta ctaataaact tttctctcca atctttatca aaagatgtaa
     9001 tatctttcaa gaagagtttt ctaacttgct gtatgatact gttgtattca ttttttcgat
     9061 ccataagttt tgacaattca tcattattta tcggcataat tatcacatta tcagtgtact
     9121 tgtccttatc cgaagaagat tggagcggaa cagacgatat agccttccat atgttaatgg
     9181 tattaaaatc aaaggaatct gcaataaaga atgttgtaac ttctctgtta gttgagcttt
     9241 cttcatcaat tttcaaatta acagagtgcc ttagaacagg ctcccattca ccacgttttt
     9301 ggctattggc gttcatcagc gtagcttcaa gcattatgac ttgcttttcc tcataaataa
     9361 ctatatcgcc cataccaccg ccagcgtgag ttaaaggctc aaaatttgct gaaagagtga
     9421 gattaaagct gtttaacaag tcaattctct tatttgagaa gtagtaccaa gctaaaccga
     9481 caatatactc atatatggtt ggaacggttg catctggata aacttcttcc ttaatttttc
     9541 catcatttga acggtcagag aacatactga gcagagtttt aacacgagtt tcgggataag
     9601 ttttatcaat aaacaaatca aagtcagttt ttcttctttg tttgatgatc tgaggaatat
     9661 cctctatttc tgcaccgtca aattcagact gcaccgcaag aagaatcttg tcaacagcag
     9721 tttctgaata ttccataatg gttattattg agtgattgct gcagtaatag ctgtcccatg
     9781 attcttcata gtctgaaata tcattaatta tttcatttga gatagttccg aagatatgat
     9841 tttttatagt ttctttatca aagatgcatt cacataaatc cctgcataca agctcaacaa
     9901 atccattgtc aaagctgata agacctgttg ccttaaaaat acgcatagtt gtatcggaat
     9961 actctctaat ggtatcaatt gtttttgagc gtgcaaacct taaatagaaa gcagtgttaa
    10021 gctgtcctgt aaacagatcg agtttttcct tctttaagaa gtcggaagat gtaggtctgt
    10081 tgccacgctt atttttgaag atgttactgc cataaccaaa agctttattg agcataggtt
    10141 tattatcttc gtatactgtt aacagttcat taagcgtaga agtatcctgt ttggtacgaa
    10201 atgcgtaaag agctttataa aatttctgat atacaataat tgcattcttg cttttttgat
    10261 ttgtaaaaat ctttgagaac acatcatcat ttatagcatt agtattattt atttcagccg
    10321 gaacagcaaa tgaataggtt tgtattatat catctttttt gtattcattg atgaatgtat
    10381 cataatcagc tatattatgg tatgggctta aaccttgaat tatctcacaa aactcgtctt
    10441 gcgaaatacg tggtttagtc aataaagcgt ataacgccat acaaaagggc gagtagtacc
    10501 tttctgcatt attatcaaat attctaagct taaaaagctg cctaagatag attatatttg
    10561 tgttgtttaa tggtaaaagt gattcaataa catctttgtg gattagaaca ttatcaagta
    10621 aaacatcacc caccgcagtt atctgtcttg cactatccgc aaaaccgagt tttaccaaat
    10681 tgcttgtata gtgtcttgca cgtgcctcta cgcttttatc aagttcgtta cgccactctg
    10741 taaattccgc aaagttatca atcaaccaat cggtatcatt ttcatcaata gctcttttta
    10801 atcggggcat actgtcaagc tgttccatca gcttatcgta ttttccgtgt ttctgaaaga
    10861 catcaggtga taaagcaatt gcttgacggt aaaaatcaag ctctgcacgg ctttcaggta
    10921 aggctttcca agtcattttt tgatttctaa gtatgtcgtt gagtattgtc aaatatcctt
    10981 gcaaagcatt tataacatcg tttcttctgc cgttagtatc cataagatta aaaatctgtt
    11041 ggccttttat cttcacgcag taacgactcc tttcattagt aatttgttat tagtacctct
    11101 tccgagctat tcgttttatc tttagtatga taattagaat tagcgtaatt ataattaagt
    11161 cggtgaacag catatttact tttatgctta tctaaccatt ctatcaatat tccgttttcc
    11221 ttgcccttac ttcgcagaac atttgataat gcacatttta tatgcagatt actcaatgtt
    11281 tcaatgaatc caagtaattg cttttctttg tcagcattcc atccatcctg ctcattgtaa
    11341 gttgcacaag ttatcagata tgggggatcg atatatacaa aatcatttcc agtcaatgtt
    11401 tgtatatcaa aatcattaaa gtccatacaa gtaaatgaag aatcttgttc tttgatccta
    11461 tcaataaaat caagcaattt tttctccatt ttcttgttga aatctctttt gccaacgggc
    11521 aaattaaact cgcccctact attaaaacgt atctgattat taaacgaata cactataatg
    11581 acatacaata aggtgtagta atagtaatct tcatttgtgt gaaaattaaa atcatcacgc
    11641 attttagtga atttttcctt attataactc cctaaaccgc tactgctatc acattcatag
    11701 taatcatatc cgtacttgct tacaagcgaa agaccatact tttcaataat tctatatatc
    11761 cattcaagcg taacatcttt gtcattgttt tttagggtat tgtaaaggct aagtaagtaa
    11821 cgattagtat cattatatat atgccttttt gccttaacgt ttatcccaac gttacaccct
    11881 ccgcagaaca gatcgacgaa tacatttatt tcttttggga ataatgggat aatttgaggt
    11941 agcagcttat attttccgcc cgtatagttg agcggagatt gtataaattc aattttattt
    12001 tccatgatca attgcactgg cagagaaaca gcctctcagc attatccttt atatcggatt
    12061 tacctgttga aaatgcttta tagctttcct caaatacttg cacttttcct ttctttccta
    12121 atgcatcaag tattacttca tcagaaattt ttgcatttga acggtcattt cctttcacag
    12181 ccatattgtt atatgaaaac agaatataac gagcattaat gttatctatc aattctctaa
    12241 aagcaacttc agcagattgt gtacagtatc gacttttcat tgccgttcta tccattttct
    12301 tcgcaacacc gaatactttt ggcttttccc aacgagcaac attttccaac agatgataag
    12361 catcacaata ttgccttgaa ttgtatgggg gatcaatata aactaaatcg ggatttatcc
    12421 tctttacaag ttcatttgca tcttcattaa aacattgatt aagagggtta tttagagcat
    12481 tgacattagg cacgctaagt accaagtgcc tgtcaaactc tatgccttgc ctatatgcat
    12541 cataatgccc acaggtgttg gcaatcttat ccattgcgta aatgagtgac ataataagca
    12601 gagccttttc ccgtgcatta atttttttct ctgtatacag caattcaata tcttcacgaa
    12661 taagccctat ttttgaacaa tcggagcgtg aaaaataggt atctgaaaaa ttttctgtca
    12721 tatagttttc ttcaataaca acagaggaat tgtatttttg aataagatga atgatttttg
    12781 atttatcaaa agattgacta ctaaaccaag cataattaca gatataatta ctgtacataa
    12841 ggtcattcgt aattatttgt ctatccgtaa acgccgatgc aacagcacct gtacccgaaa
    12901 aaatatcagc cacttgtgaa atattatgac aataactatt tacaacttcc gtgatgaaag
    12961 gtaagagttt atatttattt cctaagtatc ttctgttatt gatgctgaaa gttgaaatat
    13021 ctccagcagc acattcttct tgcacatgac taatcaaatc accataatca caattcaaaa
    13081 aagaacatat cttatcaagt atagataatg ctacatcttc atttctcttt agcttggtca
    13141 ttgtattagg agctatacca gtatattttc ttaaatcagc catactcatg tttttattag
    13201 atagcattct ccataatcca tcatatgtaa cactcattca aaacacttcc ttccaattgt
    13261 accactatca ttatatcata ttgcaacaca attcgcaata tattaagagt gattttacat
    13321 atcagccaat tgcagtatca gaaatcgatt ttgatttggt gaaaatgtta tatatttaac
    13381 ttgttattta agaaatatat ctttctgata agaaaaagag ctacaccctc cgtcacggag
    13441 agcatagccc cttgtaatca gccgtaagtg tctattttag ccgtcccaga cctcagtcat
    13501 aagtgtaatg ccgtccttaa agcctgcaat ataagcgttc ttcacctcat cggcagtcga
    13561 atcgctctgc atatcgcaca gcttgtggaa catcttgacc tgctcctcgg tcagcattgc
    13621 ttcaaattcg tcagacaggt tctcccattc ctcattgacc tcaccagcag ggagagagct
    13681 ttcacgatag ttaaaaatct catcaatcat tgtaatccgt cctttcggta tgcttaattg
    13741 tttgtgtact tcattgcata tcgaccaccg taaattcgtt gttttacgga attaggaggg
    13801 tgttggcgtg agttctactg ccgaaatggg tacgggtact aacgttcagg acaagctgat
    13861 agccgttcat gacctcgata ttccttggag accggctgat gtaggacgga ttttgcggac
    13921 attcaaaata aaaaagaatg tggaggtaa
//
LOCUS       DAWEMR010000058        13725 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1853_length_13725_cov_11.552158,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000058 DAWEMR010000000
VERSION     DAWEMR010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13725)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13725)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13725
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1853_length_13725_cov_11.552158"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12651..12660
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4556 a         2777 c         2801 g         3581 t
ORIGIN      
        1 ttttggcaag atcgcagcct tttttcttgg ggactttttt tacagcccct tttttgcaca
       61 attttcaata tccgcacaac attattgtag gggctggcgc cctcgacagc ccgtcccctc
      121 gacgtttcgt ctgcaatctc atttgtatta tccgcacaat gttgctctac acaaattctg
      181 gataatccaa accatatcgc cccgagtggg acgtcgtatt tctgcaaagc agaaattgcg
      241 ccgctcccct acatagtttg tgcgttaaat tatagcacct tgcttccgtt tgctgtaagc
      301 ttgaactttg ctcccagaac acctgccgca cctctgcaca tgagcatacg ctgtaagaaa
      361 atatcaaaat agtcatacat ggtgcagata ctttcgtcta tctgcaaatt aagggatatg
      421 acctttttat cagcatttat ctttaggctc gcttctgtta ccgcatagtt caccctgtca
      481 tggatatcaa aagaggactt atccttattt gacacacgat ttcttcgcac gtctgtttta
      541 tcacctatta tcactgcggc tgacaccggg tctgttgcgc cccctgttga ttcgtcatgg
      601 cttccaatgc aggaaactat cgtcacccta tcttccaatg gcatatcagt gtctttgaga
      661 atatcgtttg caagtattgc accatactct gcatggtgtg tacgattgac agcgttgccg
      721 atatcgtgca tataccctgc tatctttgca agctctatgg tatgctcatc atagccgaac
      781 tctttaagta ttgcggcggc tctgtctgcc acaagagtgg tatgcacctc tgaatggtct
      841 gtaaaaccca gagatgtaag atttatccct gcccttttta taaatgcttt tatttccttg
      901 ttttttctta tctctttgta tgtcattgat ttctcctgtt ggtttatgat tatatttatc
      961 tacgcaaggc tttttacgtc cacataatgt attgtagggg cgaacagtgt tcgcccgcat
     1021 ttgctcgcct gcatgaattt gcgattatac aaaccatgtc gctctccgaa cgggacgtca
     1081 tatttctgca aagcagaaat tgcgccgctc ccctacagtc actcgccgtg acgggcattt
     1141 tacgccatac attgcagcat gagatacact tgcaccgatc tttaaaatgt gtaattatag
     1201 cctgcaatta tcatatcact atccaagctt atatacaata cattccgcat ttattttaca
     1261 atattttgac actatacagg cacaaaatag ccgtcgatgt gctcattgtc aagcccctcg
     1321 ccaatgacga taaacaccgc ctgacctcgc tctataggct taacagtgat gttcttgtgg
     1381 gtggcgttaa gctcaagcca cttgccctca gcgttttgca taaagccttt tacacggaat
     1441 atccttccgc acttgctgtc gctcataagc ctgctcacag catcttccat agcattatca
     1501 ggaagcttca tattcataaa atacaaagag gtataagctt tatcttggtc gaaccacagc
     1561 ttttcgtggg aatcaggctc atagccactg ttggctatcc tttccatatc ctcatctgta
     1621 agctccgacc aatctttcat tattatctca ggctttgtca ttctgtggca atggaacttt
     1681 ttctgcacgg cttcaaggtg tgttagagta tcctccagct cctgctttgt ggcttcctga
     1741 catctgctca tgagaactat gcctgcttcc gaaagctgag aagcaagaag ataatcagaa
     1801 gcttccgaca agttggtatc aaggcaggca tctgcaatag ttataacgct tcctatctca
     1861 taccaattat caagaggttc ttctctgagc acatcaaaga actcgtcagc ttcatagata
     1921 cctgacggct ccacaatgac cctgtcaaga ccacgcatac ccatagcgat aagcttagtc
     1981 ttaaaacgcc gtctatggca atccttatca gccgccgaaa ccatttcaag gtcgcaattc
     2041 tcgtccaaaa gctcctgcaa aagaagcata tccacattca ctgcaccgaa atcattctcc
     2101 aacacgccaa ttttacagcc cttatccaaa agataacggg catatttttt tataaaagtg
     2161 gtcttccctg ctccgagaaa ccccgtcaca aggtctacct tggttttctt tttctctgtt
     2221 ttcatagtta atgcctccta atggtcatat tataccacat tgacagtgct ttggcaatga
     2281 cagaaataaa aaaaacacgc cttacactaa gtaaagcgtg ttttgtacag ggactcgccc
     2341 tacattacaa agttgacaac aaaaaacgca ccctgcaagc ccgcaaagtg cgtaaatacg
     2401 ctggtgcgcc agaagggact cgaacccccg acctactggt tcgtagccag tcactctatc
     2461 caaaaataaa atcacaaatt ttctttcagt taaataaaat atcatgaaaa ttgctgctaa
     2521 gacttctctg gcagcttttt tgatgatatt tatacatatc atcaagcata gacagggtag
     2581 ctccctgtgc ggcaggacgt tctcccctgc cgctttttct gtgcttaata cggagaactt
     2641 aaacattttc agaaagggtg tttttaatgg agaacacaaa aacaaagatc gtatccgtgg
     2701 caaatcagaa aggcggagtt gccaaaacaa catcggcatt caatattgcc gctattcttg
     2761 cacagcgaga gaaaagagtc cttatgatag accttgaccc tcagagcagt ttgtcggtat
     2821 cgtgcatgaa gtacgaatat gaccctgacg atatcactat cactgagctt atgtcatcta
     2881 tcataaataa gaaaaccgtt gatttcaaga cggctgtaca ccacaacgag ccgaacaaca
     2941 ttgactatat tcctgcgact ataacacttt caggaattga agttgacctg tgtagaacac
     3001 gctgctgtga aatggtgctg aagcgtgtca ttagtaatta catcggctgc tatgactaca
     3061 tcatcataga ttgccctccg tcactgagca acttgctcta caatgcgctt tccgccgcag
     3121 atatggtctt gatacctgtg cttgctcagg aaatggcatt gagcggaata ccgcttcttc
     3181 ttgacagcat tgacgatatc catgagtatg ctaaccctga ccttgaaata ctcggagtat
     3241 tcgcaacatg gacagagaaa aacaacacaa tgtcagctga agtaattgag tcaataaaag
     3301 ccgcattcaa tgataaatac cttggcagca taagcaagtc aaaagcagct caggacagca
     3361 gccgtgaggg catcgccctt tgcaactata aaatcagcag cacaaataaa tacaagaata
     3421 tgctcgcaga cgagtatcgt gttatagctg acaaaataga gtgtgtcacg gtgacacact
     3481 aaggaggttt caatatgata gatataaaac gctccgcaaa tcgtccaaga gctaacttct
     3541 caaataactt tgaggagctt ctcatggata agcaaaaggt ggtttcactg cctatgtcaa
     3601 aaattgagcc tttcagcttt aatggtgatc cacagccctt tgcaatagat gaaaatgaga
     3661 tatctgcact tgccgactct atttcaaagt gtggacttat cacaccgata atagttcgca
     3721 gcattagcag caacagctat cagatactca gcggtcataa aagatacgct gcttgcaaaa
     3781 agcttatgca ccttgacatt gacgccgttg ttatagaggt cactgatgat aacgtggctt
     3841 tcgatatagt ctgccaggca aacattcagc gcaaagagcc acgtccaagc gagctttcaa
     3901 aaatgttcaa tacatactta agtctgagga aaaacgtttc agcagacctt acagtaaatg
     3961 agataagcaa tatgttcggt atatccagaa aaaccattta ccgctatgca aatatctcaa
     4021 atcttaataa tgggttagca gagctgatag acagcaaatc tattcagata agattcattg
     4081 agccgcttgc aaagctcagt cctgagcaac agaaagccat agcagactat tacaattatg
     4141 acgaacggct cagttcaaaa gagctgacat tggtcataga gcaaatgaca aacagtggaa
     4201 gcagtgatat ctatgatatc ataaaagcta taagaaatct gtctgaaaag aaaaaagagc
     4261 catacaagaa cagcttatac aatgagatgt atatcaaaaa gccgttatgt atgcagaaca
     4321 tgaccgaaag tgagcttgac aagatagtgt atgagctgct tgataaatat cttgaaaata
     4381 atttcaagag tacaaaaaaa gtcaccacaa acgaaaaata atatgatata atcatagcac
     4441 aggaggaaaa gtaatgaaca tgacgttcaa aatagacata gaccgcagaa aagttgaagc
     4501 tatccgctgc tatatgccag acggaaacat cgaagagttg ctcaatgagg agataaccaa
     4561 agccgcagaa aatgttgtga acaggcttta cagcaagact gttccgaaaa aggttcagga
     4621 atatctcaga aaatcagcgg agattaaaac tgatagcaag aaaaagacgg agcaagtcaa
     4681 tgagtagcgg tgtcataaaa acatttggca gcaatcatca aaccgcttta tatatggcta
     4741 aagacaaacg gctgtatgag atagtatcaa tagacaatga ttgtgctgta atggcggcgt
     4801 ttaatgatac atttgcggtg gctgcgaagc cgttccttga cgaagtaaac aatgtaatag
     4861 actattcgag cattgaatac tttgaccgtt tatcacaggc gaaaagctac agtgatagac
     4921 ttcataaatg ctttgacagc tccaaagcag aagaaaacat caaagaacaa tgtgtcacgg
     4981 tgacacactc agataacatc actcaaaagc ataaaagttt gtaattgaaa gaggaggacc
     5041 attatgaacg aaaacaggaa ttttaacgaa gattttgaaa tcgagcaggc aggaccagaa
     5101 gatgttggct gcgatctttt tgagtatgag cttactccag agggtacata cgtcccgaag
     5161 atagcataca caagtgaccc cgaagaagaa aagctgctta agaagcctat ccgcagatgg
     5221 ggacgtgcat ggatgaaatg gatggaagca gaacacccag cagacgttga cattctcgtg
     5281 tgcgaatgcc gttggcagat cattccgaga gaaatagata tcgaagctga ggaaaggttt
     5341 gacgagcttg acgaaatcta cagaaaagat cacccacgtc ctacagagtt caatgctata
     5401 cgcaagtggg aaagggaaag gcttatgatg ctcgaacatc aggtaatgga ggagatcgtt
     5461 tataagctcc gtgaataggc tcaataagga acacatggca cgttgaaaac gcaacagaaa
     5521 acaggtaaag gacacttgat atttcaggtg tccttttttt ataaaaagga gtgattggac
     5581 aatggaaaaa ataatttatg gttcactggg aatagctctt tgtataacag gaatttggac
     5641 tattataagc gcatttattc ctgaaaaata cagacttttc aagaaaggtg gaaagaataa
     5701 tgatattcta tgatgaagaa cacgaggaaa tgttcaataa gctgtgtggc aaaatgccct
     5761 atcttgacgg ctatcacttg tcgcttgcgt atctcctcac gatggacgct gttttgcgtt
     5821 atcatattgg cgcactgtat gacgttaaaa aagacgtgat tattttcgag ggcttgagca
     5881 agccttttca gacgcatacg agcttgaaaa caacacgcct tgcatttaat ctttggaacg
     5941 gcacggttta tgactcagat ccacctgaaa cgtatatcaa caaggacgga gagaaagaat
     6001 atgtgccaag caagtattat gcgcccgaca atattttcaa ctgcacctat gcaccgtact
     6061 actttgaagc gctcaagatg aggttcgaga tttttatgta agaagatttt aataaaagga
     6121 gtgatatgac aatggctgaa ttaaagtgca aaaaatgtgg cgatattctt aatgatgata
     6181 attgtgatga attgtatcac ttgcatataa acaatataga cgttgataat tcaaccgagc
     6241 tgatgtgcaa cgattgtgct tttagagaag atgcaagaaa acacgttcag gaatttatat
     6301 gtgatgatga aagtcttaaa cttgatgagc gtatgtttat ttcattcaag cgtgttatgg
     6361 aaaaatataa gatagaggag aaatggcgaa actatcttaa tgaagtcttt tcgagtggcg
     6421 aagatccgac gaattgtagt tttgacaata ttgaagaatt tatttgtgac tctgaccaaa
     6481 caggcaatgc agacgataag cttgattacc tcgcagacta ctattatggc gagaattggg
     6541 aactttatgc gcaatttcaa gattggtact atacatactt tggtggcttt tgggttacgg
     6601 actttgaaga tgattatgtt taatataaaa agaaaggcat agtgagcaat tatgaaaaac
     6661 aaatatatca ttattgacac agaaacaact ggctttcacc catatgacgg ggacgagctg
     6721 ctgcaagtat ctatcataga tactgacgga aatgtattat tcgatgaata tttcaagccg
     6781 cagcaccgta ccgaatggaa agaagctgaa caggtgaacg gcataagtcc tgaaatggtc
     6841 gctgatcgcc ctgctatttc tgaaaaaata tcagagataa atgctattat tgaaaattct
     6901 gatactataa tcgggtacaa cacgcagttt gacattggtt ttcttaaagc aaatggtgca
     6961 atagttcctg acgatttaga aacggtcgat gttatggagg acttcgccaa gatatatggt
     7021 gaatggaata gtgcccgtgg cagctacaag tggcagaagc tgactagagc cgcagagtac
     7081 tacggctatg actggtcaca gagagctgaa actgcacaca acagtttggg cgattgctat
     7141 gcaacgcttt ttgtgtatca taaaatactt agcgaagaat agcagccctg agcaatgata
     7201 tcgcaaacgt gataaacgct cagaattaaa cagtaatatt tgacatcaca aatcaattgt
     7261 gatataatct agtttggaaa aggtaaactt aaacggtagg cggtgtcctc ccacacggaa
     7321 tggtctgaat attgtcagcc gaaaggctca ggcaaaatag gatattcggg aggtgataga
     7381 atggacttag ctttctttgt cttgactatc gtgctgattg tcgctctcaa cgagttgata
     7441 aaaagcataa aaaaataacc gccctcgacc aaaagttgcg gttatttttt acataatcaa
     7501 aaactgagga tagccgtcta tgcggtttac cttttctata tataagtata catcacttta
     7561 agatatatgt caagtctaaa aaggaggaaa accatatgaa gaacacaatg aaagccatag
     7621 ccgccctgct catcagtgcg acaatgataa gcataaccgc ctgtggcaac acagacacca
     7681 catcatcagc cgcaccgaaa gccacaacaa cggcttcaag cacagaagct tcggcaacta
     7741 ccacaacgga agccacactg acctctcaga gtgaggaaac aacgtccctt tcggctacca
     7801 caactacatc tggaacgagc aacacttcta caagcagtgg taaatcttcg acaactacca
     7861 agggtggctc gaccgcaaag gcgaacagct ctaaggctag taatggtgga acagtaaata
     7921 atcagagcta cagccacagc accgcaaaca acggctcagg caacaacagc tacagcaatg
     7981 acaataacaa tggtggcaac agctatcagc cacagcagac ggaacgtcag acagaaagaa
     8041 agacagagcg tcagacaaca actacaagac agaccaccac aacggcaaag aaaacgcaag
     8101 ctccaaagcc aaagccgact acaacaacgg ctaagccaaa accaaaattc gagcttactc
     8161 agagtgatat agacagagcg gtaaaagagc ttcagcagta cagcaatgaa cagtgcgact
     8221 ttgacgctat cttctataat gagcaggaaa tgtgggataa catagccaca agaaatcttg
     8281 acaacagctc atggggaaca cctgacactg tttcgcctga tacttattcg tcttatgatg
     8341 aactttatcg caaagtaaaa ggacacattg atgttctata tgacagaata ggcaaagacc
     8401 aagcacatat agttgtgtat cagcaatggc atggtgacgg ctcagctatc aacagtgacg
     8461 gcaagcctgc atgggaaata tacctccttt actgatatat aaataaaact gaatagagtt
     8521 taaaagcaat tcgattttct ttcgggttgc tttttttatt gcccgaaaat caagccttat
     8581 ggctttttag atagataaaa aatttttagg aggaaaacaa atgaaaaaga aaagtctttt
     8641 caaacgtctt agcagtgttg cactcgcatt tctgctcagc ttgtcggctc ttacacaagg
     8701 cataacagcc agtgcgttct ccgacaaaaa ccaagccaca cagcagtggc tgtcaacatg
     8761 gagctatgat ttccgtggtt caggtctgcc aaatggctat gatattgaaa cagggaaata
     8821 caatgacact cacttcactg gatactttgc atcagaagtg ccgtatttca caacatctga
     8881 taacaactac gcttactgca ttaactttga taagctgcat aatagcggaa cagtttcaaa
     8941 gcagcagaca cttaatgatt acatgaaaaa cggtcatatt cctgatgata tgcagtatca
     9001 gataaaagag ttctgtattt acagttacaa aggcaaaact cagtacggct attctaagga
     9061 caccgaactg tgtgcgtcaa gtgttatgtc acaggcggtt tctgcacgtt tttttgatga
     9121 aagcacaaca ggactttcaa gcaatgaaac aaagcttctc aacgcctaca gctacaacgg
     9181 cagcaacgca agcagtcatc acaatgacct tgtcgattgc tacaaaaaga tgaagtctga
     9241 aatgctttcg cactacaata ttcctagtgg tacgtcaaag gcttcttacg gcaagccact
     9301 tgacaaagct tcatatcctg caaaatatga tgtcaagaca ggcaagtgga cagcaaagct
     9361 caagcttgac agcctttcac agttcagcgt ttcaaagccg aactcagtga ctgttacaca
     9421 ctcaggctca aatatgacta tcacagcaga caatgagaaa gatctgaaaa acgttgtttt
     9481 cacgctcaca aagacaaagg gcaagtatgt tgaacacata gacgagtgtt cacctcttat
     9541 cctttcgggc ggctcagacg ttcaggaaat ggtggtttcc tgctatgagg ataaagaccc
     9601 tgttaaggct tatctgaaaa taacaacgcc aacaggctca gctcacatca acaaaacatt
     9661 cacagattac ttcggcaacg ctcagacagc cacagcggat atgtacaagt ccgttaagtt
     9721 ccagttggca tacaaggacg gcaacagcta taagtttgta aaggcaactg gaaaggacgg
     9781 aagctacagc ttcactgatt tggcttcaca ggctacagag cttacgccga acagtaaaga
     9841 cggcactatt gatatcacag gactgccaga gggcagctac gagtggcgtg agatatccac
     9901 agcagacgga tatatgatat catcgaagaa agctatcact gtttccgctg acaagacggc
     9961 tacaactgag ttcatcaaca aggcttctgc tcctgacaag ggcaagataa ccattcataa
    10021 gcgtgacgct gaaacaagag ctgaccttgc aggcgctaag tttgaagtgg cagcagccga
    10081 ggatatctac gttggcaacg gatattgtat ctacccgaaa ggaacagtta ttgacaccat
    10141 aaccacaggc acagacggca tagctgaaac aacaaaggct ctctacaagg gctacagcta
    10201 tacagtgaca gaaatcgaag caccaaaggg ctattcgata tccgatgagc ctacacagac
    10261 tatcaagctt gttgaacagc aggctgagtt tgttgttgat tttgacaatg aacaaaacac
    10321 tattcctttt gagataacaa agactgacat ttcaacaggt gagcttatcc ctgattgcac
    10381 ttttgagatt ttaaatgaaa ataacgagca ggtcatcaca ggcacaaccg atgagaacgg
    10441 cattgcacat tttcagctcg ccataggaaa gtatttctat cgtgagattt cagcacctga
    10501 tatctacgag atagacgata ctccttatcc ttttgagata actgaaaacg atgatatcgt
    10561 caaggctaaa atgaccaata agaagaaaag tggctctatc aaggtcacaa agtctactac
    10621 aggcaatctc aatattgagg gcattaagtt ctttgcaaca ggtatttccg atacaaacaa
    10681 ggacatgaag tttgaagcta caacagacga gaacggcgtt gcactctttg ataagcttgt
    10741 tatcggcaag tacacagtaa cagaggacgg ctcaacagtt cctgcggctt acctcactgc
    10801 tgatgagcag gaagtaactg ttgagtataa cacaaccgca gaagtcaagg tcactaatga
    10861 ggaaaagaca ggctctatca aggttcagaa aaggactgag ggtcagaaga atgttgaggg
    10921 tatcacattc tacctcaagg gtacatcaga cagcggcaga gagataaacg ttcctgcagt
    10981 cacagataag gacggcgtag cggtctttga gaacgtaccg atcggcacat acactatcac
    11041 agaggacggc tcaacagtgc cttatggata tcttgtcgct gatgaaaaag aggtcaaggt
    11101 agaatacgct cagactatcg acgcaacgat aatcaacgag gaaaagacag gctctatcgt
    11161 agtccataag actacagagg ggcagaagaa tatcgaggga atcaagttct atctcagagg
    11221 tacatctgac acaggacgtg aaattgatat ccctgctaca actgataaag acggcatagc
    11281 caaattcgag aacattccta ttggcacata caaagttatc gaagataagg aaacagttcc
    11341 gtatggctat ctcgtagctg atgaaaaaga agtcaaggtt gagtatgctc agacaattga
    11401 tgaaacaatt ctgaacactg aacagatggg tacagttcag gtacacaaga aaactgacgg
    11461 catgacgaac attgagggca ttcgttttat tctttcggga gtttccgata caggtagaga
    11521 gatacgcatt gaagctgtta ccgacaagga cggacttgca aagtttgatg gcgtacctat
    11581 aggtacatac actatcactg aggacggctc aacagtgcca tacggctatt tggtagcaga
    11641 ctcaaaacag gtcactgtaa catatgctca gacagtagac acagaaatgt tcaatcagaa
    11701 agttcctgac acgccaaaca ctggcgtttc cgacaatgac attgacggca gaacagttct
    11761 tggcggtgtg gtagtcatac ttgcaggagc tgctatcgtg atgttcagcc gaaagcgaaa
    11821 agaaagataa ttgatgctgt ctgaaaaaag acagtaaaaa ggggatcaat ttatcatata
    11881 aaaggaagaa agcccctgca actgggcagg ggctgggagg gtgagaaata tggacactct
    11941 ttttttgatt atatgctatg tacttgtaat aggatcttgg tctttgttgt ggtcaatata
    12001 tactggtgat cctttatata tttggcatag gcagatcaaa ttcgtgaaaa agatatacaa
    12061 tcatttttat gaaaattgga aaaattacaa ataaaagcgg agttgattct agtgcaagaa
    12121 ttgcatattc gtagtatttc catacacctg tcatttatat taccaaaccg ctcattgcct
    12181 acattaagaa ctataaaata ttgttctaaa tagttataaa atatatggca agccaaagct
    12241 gctaaacaat taaaaagcaa caataaacaa aaacgaagta tatttaccca gtgcttattg
    12301 tttccatcat tgtgacaatc attatgccac aatggttcaa agcctaacac ccaaccatat
    12361 attaacagag gtaattgtaa gatagcaagt atattgcata aatggtcata atagttgttt
    12421 gatattacaa gtattggtcg aataaacaaa aatatcaaca caatcgaaat taaatatgcc
    12481 aatgaccgat attgtttttt attacgcacc aaatcaataa aacacacgcc acttgtgaca
    12541 aaagtaataa cggaaataac gttttttatc actaactaaa cctcatttca ggagaaaaaa
    12601 tgaaaatatt atattatatt ttattagcct tttatttgta caattttgta nnnnnnnnnn
    12661 gagttgcata tgtacttttg tggcgttcct tattataaaa gtttgatagg agctgataag
    12721 aatgttaaag atccgaatag agggtttacc cgaagaagta gaaaaagtca cagaactgtt
    12781 agaaaaagac gagaaatacg aatttcttca aaaaagtgaa aactatcgga gcagaagttc
    12841 ggtttatgtc agaaaatatg tggagataag gttaaaagaa caggagtgag aaaatgcaaa
    12901 aagaaattga aataattaaa gacggtttgt catacaatga ggaacttgag tgcattgcaa
    12961 aacgttttgg tatgacgcag gagcaaacgc ttaaagagat aattcatgag atatacacaa
    13021 gctcagtgac ttttacaggg ctacaaacag ccatgatatt gataggggac tatgaggaaa
    13081 aaagcagttt cgttgagtat aactctgtcg gaaatgtaat agttgatgac aactgcaatg
    13141 aagacgaccc atatgagggt aataccgctt tttaaaatgt tattctaata atccttatat
    13201 atctcatatt cgtaaatttt cattatgata acaggaatgt atgaagctaa aaaaagcaat
    13261 aattgatacc acacaaattg tgaaattaca tattctaatg caaaatccat acttgtatga
    13321 attgacataa taactgtgag cagataaaga ataatcaaag acggaacaat catattatta
    13381 acgctattta tattcttttt tagatttata tgcttatata gtatatcatc aactgaaata
    13441 ttgtattggt gcatatctat tgaaaatagt aaagaataca agccgtatag aaaaatatca
    13501 agacaaataa tgataataat tattgcttga ttaaatttaa tactcatttt aataatatca
    13561 tacgagctgc tatcataaaa taaaagttga tcaagttttt gcttgaaagt actgtctaac
    13621 aaacaaagtt gcagtgcgaa tgaaacaaca accacgaata cacagctttt tttagccgca
    13681 cagataatat tttttattaa acctatcatg tttacacgtc ctttc
//
LOCUS       DAWEMR010000059        13629 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1876_length_13629_cov_11.222484,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000059 DAWEMR010000000
VERSION     DAWEMR010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13629)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13629
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1876_length_13629_cov_11.222484"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    211..220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3535 a         3002 c         2922 g         4160 t
ORIGIN      
        1 gacccttttt cctgcgataa cagggccttt atcctgtgcc gctgcgtcat attccctata
       61 atagctgtca ggtatcttca gcacgaaatt cttcttattc ggcagctttt tcacagttcc
      121 gtctgctgaa actgccacag agatctcttt tttagtgccg tttatgtgat ttttctcttt
      181 ggaaaatggg ataatgacct cattatcaga nnnnnnnnnn gtggagcttt tctccgtttc
      241 atcaactttt gccatgctga cagcttcagg ctttttcttt tcctcgccta taggctttcc
      301 accgaaagta acgctgtcaa gatactctgt ataggcttct gaaagagcct ttgtatcagg
      361 cagtttgaag tccttttcaa aatctttatt atttgccata ctctttcacc tgcctaaaat
      421 ctgaaataca agaacggatt gtttgttgtg ttcacaagca gaacgcttga gagaacaagt
      481 atcgccgcat tgcataggat acctgctgac tgcataaaat aggctgtacc ctttgcagag
      541 agcgcctttt ctttgatctt tggtataaca ggcattgtga aaagcactgc cgctgcaaaa
      601 agccaaatat tgttcatgaa aaggtgctgt gtaactgtgt cggtcagctt gtttccattt
      661 gctccaacaa gtgccttgaa gaacgtaccc agcgagccga ggttctcaaa atagaatatt
      721 ccgaagccta tgaaaataac aagctttgta tagatatgtg caaggggaac aggcattttc
      781 ttcatgcgct ttttgcctat gagcatttca aagaatatga acattccata gtaaagtccc
      841 cagaaaacaa agttccagct tgcgccgtgc caaagtcctg tgcagaacca tacaaggaaa
      901 agtcctccgt attttcttct tttgccgaag atagggagat aaagcacata atctctgaaa
      961 aatgagctaa gggagatatg ccacctctgc cagaactctg agatagtttt gcagataaaa
     1021 ggatatttaa agttctcatc aaagtggaag ccgaatatcc tgccaagacc gatagctata
     1081 tctgaatagc ctgagaagtc aaaataatac cacagaccca caagaattgc gccaagccat
     1141 gtgcctgcca cagagagggt ctttatattt tcatagccgt ttgaagcaga gccgaaaagg
     1201 gctgttacaa tggtgctgag attatttgcg ataatgacct ttttgccaag tccgaggatt
     1261 attcttgaaa agccgtcgga gatatccttg gcggtggtct ttctttcgtt tatctcctcg
     1321 ccgataacgg agtatcttac gataggtcct gcaacgagct gtgggaacat ggatacatac
     1381 aaaagaaatc tgccgaagtt tttctgcggc ttgaccttgc cccaatagca atcgacggta
     1441 taggaaatag tctggaacgt atagaaagag ataccgatag gcaaaacgat attaggcacc
     1501 ggtatgccta gaccgaacca attgttgaga ttttccacaa agaacgccgt atacttgaaa
     1561 ccgaacagca ccccaaggtt aaagacaagc gaccatgcaa gcgccgcttt tgcctgccag
     1621 ccttcggggt gtttttctat cacccgacca tttatgtagt ccacaaggga ggaaactatg
     1681 aggatactaa cccagacagg ctcgccccat gcgtaaaaga taagtgaaaa caccaccagc
     1741 actgcatttc ttatggctgt actgcgtgtg ataaaataac atatcaggca cacaggcagg
     1801 aaaaaataca agaaaaatag atctgcaaat accacaacaa aactcctaac aaaaattgat
     1861 aaaacagcca ttgtcggctt attccgacaa aaggcgatac acatttaagt atatcactga
     1921 acacattaaa agtcaattaa aacagaggtc tgcggaaagt ttttcggtta tgcacaaaaa
     1981 aagccgcaca aattgtacgg cttagaataa tatgaaaatt tattctttac aaattgaaag
     2041 ggcaagcaca aacgggacgt cggatctctg caaagcagaa attgcaccgc tctcctacaa
     2101 taacattgat agtatttatc aatactttat tttcagggtc ttgcctggaa cgctcacgcc
     2161 gtctgacaga gtgataggga gcttttgctc ctcagagaag ccgatagttt cgccgtcttt
     2221 aagctcaacg ccctcagaaa gcacatactc ggagatagaa agcaaaaatc caagcagatc
     2281 gttaggctgt tttggagagt caactatttc catttcctca tatccgaaaa atctcatgcc
     2341 tgatgtgaaa gcacaaagac ctgttttagc aagatacata cctgtgtaga taagaatagg
     2401 catcggatac tcgccgtctt tgatagtttc ggcgaaattc acatagaaat tcttctcata
     2461 aactgtcggg tctttgtaga taccaatagc gttatcgagc ttgagcagag agcttgccac
     2521 ctttgcaaag aggatagcct gctcaacagc gcttgtttta ttcattacag cgagcataac
     2581 gtgtgcctga tgcttagcga cctcgtttgc gccctctttc cagagaatgt tatactttgc
     2641 cgcattttca gcttccttgt taggaactgg gttaggcata agtgagcagg cgaccaccat
     2701 atcgccaacc ttgaatacca tagcgttgtc ttttacatcg tcctgcggaa cgatgttcca
     2761 atcgtctttc agaaactttt taaatctgag ccaatccatg ttagcgtctg tgtagagaac
     2821 gaagcctgca aggacgttgc cttccttttt atcgtccttg gtttcctgaa cgtttttgtt
     2881 ttcttctgac attttgttac ctccataaga taaagtctgt tgaggtcatt atagcacact
     2941 tggggagaaa atgcaacagg gggaggtaaa tgaggaatga ggagtgagga atgaggaatt
     3001 atggtatcgc ctgcggcgat gatttttttg tcctttcttt atcgccattc cccttaataa
     3061 tttgtatacc atgtacttga caaaaacgcc gttccgactt ctcgtcagaa cggcgtggtt
     3121 tttattctgt taccgctgtt ccctcagcct gctgtgtttc aggctcattg aaaggcagtt
     3181 ttactgttat ggtcgtgcct ttttccagtt cgctttcaag ctgtatttcg ccgctgtgat
     3241 atgcgactat atgcttgaca atggaaagtc ccagacctgt tccgcctatc ttctttgaac
     3301 ggcttttatc aactcggtaa aaacgttcaa agattcggct ttgatgctcg tcggatatgc
     3361 ctatgcctgt gtcgctgaac accgccacgg ctttgccgtc ctctgcgccg accgttactt
     3421 tcaggcttcc gccctcatga ttgtacttta tggcgttgtc cattagattt aaaaacagtt
     3481 ctgtgatata gctctgattg ccgtatatct gaacaggttc gccctcaatg gtcattgtga
     3541 cttctctatt atctgccttg tgacggagta tctcctctac gtcatgggcg gttttcagca
     3601 ggtctatctt atcgtttaaa acgtgttcct gctcctctat ctgggaaagc ctgataatat
     3661 cgtttataag atacaaaagt ctttcgcttt cacgatatat cataccgccg tatttcttca
     3721 cgtcctcttc cttggtgaaa atgccgtttt caagaagctc gccaaagcct tttatggtgg
     3781 taagcggagt tttaagctcg tgagatacgt tggctgagaa gtcacggcgt atctgctcgc
     3841 tttgtatctc ctctgttacg tcaacaaaga agattatgat accaaaatca agggtatttt
     3901 cgctgtagac cttatttgca aaggtacggt aaaaacggct tttttctgtg atgatacctt
     3961 ttgctgcacc gctttccttg gcttcttcaa gcagacctat tatctgctga tcgttggtca
     4021 tatggtttat cttggtatgc tccgtagggt caaagctgtt gccgagaatt ttcaaggcgc
     4081 ttttatttac cgaaagcact gtcatgtcct cgtcaagaag tatcatgccc tcaaccatat
     4141 tttcggtgat aagtgatatt ttttctctct ctgcctttat tttattcatg ttcttatgta
     4201 ttcgtcccat aagaacgtcc gcatttttta tgataggttc aagttcttca taaggcgttt
     4261 caaactgacc gatattctcg ttcctcagat caagctgttt tacaagggaa tttatcggct
     4321 ttatcatgct ttctgaaagc tttatggaga ttatggtggt gataaagatt attcctcctg
     4381 cgaacaccac cagcattggt atcactgcaa ggaacacccc tgcaaggttt tccttatctg
     4441 tagaaaatct cagaatgcca ctgtcatatt ttatggcata ataatatgta gtatatccaa
     4501 gggttttgga aaatcttgtg gattcgccct cgccatttgc catagcctct ttgaactctt
     4561 cacggtcgga gtggttttcc attttggcgg gatctgcttc agagtcatat tttacattgc
     4621 cctgtgcgtc gataaaggtg acacggacat catattctac tgatctttcc aaaatatcag
     4681 ccatttctga gggtttattg tttcctgcgg caacgagctt tgcagcggat ttaagctcat
     4741 ttttagtgaa acgctgatag gtagaatatc caagagagcc tgctactaaa aggaaaaaca
     4801 gcactgtaca tgaaacaagt atcgtcatga ctttatagag ttttttctgc atatgctgtt
     4861 cctccttatc tgaaaatttt tatattttgt agcctatgcc acggactgtt ttgataagat
     4921 ccgtacagcc tgcggcttca agcttttgtc tgagggtctt gatgtgcata tcaacagttc
     4981 ttgtttcccc tgcaaactca tagccccaga ccatttccat aagcttatca cggctcacgg
     5041 ctatggactt atggcgcata agatacatca aaagctcaaa ttccttatag gtcaaaacga
     5101 cctcctgacc gttatatgta cagatacgtc ttgatgcgtc aagggatacg ccctgttctt
     5161 ctatgacatc ggcttcggca ttctgcttgc ctgcacgtct taaaacggct tttattcttg
     5221 aaagcagttc cattacgccg aacggctttg ttatatagtc gtctgcgccc atttcaagac
     5281 ctgacacctt atcaagctcg cttgattttg cggtaagcat tataacagga acgtcctgag
     5341 tgtggctgtc ttttttgagc tttgaaagta tggaatagcc gtcctcgtca ggaagcatta
     5401 tgtcaagaag caccaacgaa ggctctttgt ttcttatctc ttcataaaac ggttttgctt
     5461 ttggaaatgc tttcacctca tatccgcctg atttaagtgc gcaggatata agctctctta
     5521 ttccgtcatc gtcttctaca caatatataa gtgccataga tcttacccct tttcgttaaa
     5581 actctttagc aggcagagcg tatttctcgc tgtagcgctt gacctgtccg tcaaagccct
     5641 cgcctgaatt tttaagcatc tgaacatact cgtggacgtc aaagctttcg tcattttcct
     5701 gtataaggca gaccgctata tttgagcaat ggtctgaaac tctttccaaa tttgtgagca
     5761 gatcggagaa cacaaagccc atttctatgg tacactcgcc tgtttgaagc ctttttacat
     5821 ggcggttttt aagctttgtt cttatcttat ccaccacttc ctcaagcggt tcaactcttg
     5881 tggcaagctc cagatcgtgc ttttcaaagg cttccatggc gatatccaaa acatctctta
     5941 cagcactgca catgactttt acttcggctg ttgcctgtga tgtaaaattc agatccttct
     6001 gctgtttttc ctcggcaacg gacaccatat tcatggcatg gtcggagata cgctcaaagt
     6061 cgcctatgac atgaagtata gttgaaactg actgagaatc ctttgcagaa agatctcggc
     6121 tcgaaagctt tacaagatat gtgccaagtc tatcctcata gaggtcgacg gctgcttcat
     6181 ttacggctat ctgctctgcc ttttccttat cgtacttatc gacgcagatg ctcattgcca
     6241 tgtctatgct ttcttttgct atctcgccca tgcggtttgt aagctctctg cacttttcaa
     6301 ctgcaaaaga cggtgactgg agaaatcttt cgtcaagaag cttgaactgc tcttcgatat
     6361 tgtccttatt tccctccccc tctttgtctt tgacagtgag gtaggcaagc ttttcaagga
     6421 ctttgataaa tggcagaagc acgatagatg ttgacaggtt gaatatagta tgcacaatag
     6481 ctatctcctt tgcgcccaca actccgtcaa cgaacgagaa gccgataaag acattgagca
     6541 gatagaaaac tgtaaggaaa agcactgttc ctattatatt aaaatagagg tggacaactg
     6601 caacacgctt tgcgtttttg cttgtgccta ttgatgaaag cagtgctgtt acgcaagtac
     6661 ctatattctg tcccaaaatt ataggaagcg ctgagccgaa tgttattgca cctgtggctg
     6721 agagagcctg aaggatacca acggaagcag atgaggactg aatgatagcg gtaagaagtg
     6781 cgcctgcaag aacgcccaga atagggtttt tgaaccagag aagcacttct gcgaactcag
     6841 gaacgtcttt aagaggtgcg actgcaccgc tcattgagtc catgccgaac atgagcactg
     6901 caaagcctac gagtatagat gcgatatcct ttttcttatc gttttttgag aaagtcagca
     6961 ttgcaacgcc tattatcgca aggataggcg agaacgagga cggcttgagc atattgataa
     7021 taaagctgtc gccgttaagt cctgacaaag acaatatcca cgctgttacg gtagtaccga
     7081 tatttgcgcc cattataacg cctatggcgg aatggagctt catgatgcct gcgtttacaa
     7141 agcccaccaa cattactgta gtggctgagg aggactgaat gaccgctgtt acagaaagtc
     7201 caagcaacat tgccttgata gggtttgagg tgagcctttc aaatatcttt tcaagctttc
     7261 ctcctgcgag tttttccagc ccgttaccca ttacattcat tccgtaaagg aacattgcaa
     7321 gaccgcctat aagggtcaga acattaaaaa tcgtcatact gacactccct gataataatt
     7381 ttacactatt gttacaatgt ttacaatgaa cattatacaa cataattgta aaataacaaa
     7441 tcaggctgag taaaatctga gtaaaatcgt acaagaagag caaaaaggcg gtatttccgc
     7501 cttttatcgt gatattttac attattctct ctgttttatg gacgcccgtt atggagaaca
     7561 ccacccattc ggcgatattc acagcgtggt caccgatacg ctcgaagtat tttgccacca
     7621 ttagggtatc tatagctttt tcaccgtttg ctttatcggc tgctatctcg tcgattatct
     7681 tatccttggt ctttacaaaa agctcgtcta ttttatcgtc agagcttatg acctttcggg
     7741 caaggttctc gtcgtcgttc acataggcgt cgatagcgtc gttcaccatt tgtgtggcgg
     7801 ctcttgccat ttcactgata tcgctaagat cccatttctg ctttttcttt ataagctgga
     7861 tattcagatc tgcaatatct gctgactgat cgccaacacg ctcgatatcg gttatcattt
     7921 taagtgcagc ggacacacgt ctgagatcct cggcaaaaac aggctgctgc tgcaagagca
     7981 ggttaagaca gatcctctct acctcacgct ccgcctcgtt tatcttggcg tcaaactcca
     8041 ccgtttcacg ggcaagcttt gtattctgct cttcaagaga ttttattgac attgcgatta
     8101 tctgctctgt cattgctgcc atagaaagca gctttttgtt aagctcgctg agcttattgt
     8161 caaaattcgc acgcatagat atttcttcct ttcacctata tttccaaagc gtttatccga
     8221 aacgtcctgt gatatactcg agagttttct gttccttagg attggagaag atacgctgag
     8281 tttcgtcgaa ctcgactatc tcgccatgga ggaaaaaggc tgttttatcg gatatacggg
     8341 tcgcctgctg catattatgg gtgacgataa cgatagtgta attcttttta agctctgtgg
     8401 caagctcctc tattttgata gtggagatag ggtcaagggc tgatgtaggc tcgtccataa
     8461 gaagcacctc cggctttacg gcaagtgctc ttgcgataca gatacgctgc tgctgtccgc
     8521 ctgacatacc cagggctgac tttttaagtc tatcctttgt ttcgtcccac acggaagcct
     8581 gacggagagt tgtttcgact atctcgtcaa ggtcggcttt tttctttatg ccgtgagttc
     8641 ttgggccgta tgcgatatta tcatatatgg acatcgggaa gaggtttggt ttctggaaaa
     8701 ccattcccac acgctttcta agctcggtta tttccatatc tttatagata ttctccccgt
     8761 caagggtaaa atcgcctgtt atcttacagc cctccacgag atcgttcata cggttgaggg
     8821 atttaagaag agtagatttt ccgcagcctg aagggcctat gaatgcggtt atttcctttt
     8881 cggctatgga caagtttata tttttgagag cctgaaaatc tccgtaaaag agatcgcagt
     8941 ttttcacctg cagttttggc tgtgtcattt atcagtttcc tcctttggaa agctttctgg
     9001 caacaaggga cgcaagtccg ttaagaagca ggactactgc cagcaggaca actgctgtag
     9061 cgcttgcttc gttcatataa agtccctcgt tggaaagtga atacatatgg atagaaaggg
     9121 tacgtcctga gtctgtaagg cctgcatact tggcgatagt tcctgcggta tagataaggg
     9181 cggctgtttc gccgaaaatt cttcccattg caaggataac gcctgagagg ataccgctta
     9241 ttgcaggagg cagaacgata acgaagatag ttctgagctt tcctgcgcca agtccgtagc
     9301 tgccctcacg gaagctgtcg tctacagatt tcagcgcttc ctcggtagaa cggattataa
     9361 gcggaagcac cattatcgcc aatgtgcaag cgcctgagag cattgagagc ttccagttaa
     9421 gggcaagcac aaagaacata tttccgaaaa gtccgtacac tatggacggg atacctgaaa
     9481 gagtttcagt agtaagtctt atgactctga cgaatttatt gcctttcttt gcatactcaa
     9541 cgagatatat tgcgcagaat atgcccagag gtgcggctat tgccaaggac accagaagca
     9601 tttcaaaggt gttcacgata gcggggagca ttgacacgtt ctcggtggta taggtctttg
     9661 cgaagaggga cggtttcaga taaggaacgc cctttatcac cacatagccc acgataagaa
     9721 cgaacacgct tatggtaagc acaactgcta ttttggttag gatattgaac accaatgaca
     9781 tggggtgtgt tttataggtt ttcattctgt ctgacatttt tatcaccctt tatcctttct
     9841 gtttgtgaca aggttaaagc aaaggttcag aagaagaatg aacacgaaca gcacaacgcc
     9901 tgttccgata agagcgtcat agtgaagtcc ctcggcgtag ctcatttcga ttacgatatt
     9961 gcccgtgagg gtacgaacgc tgtccaggag ggagtgtgga atgatagtgg agtttcctgc
    10021 tatcatgata actgccattg tttcgcctat tgctctgccc actgcgagga taacgcttgc
    10081 gagtatgcct gactttgcgg caggaaaaac aactgttgcc actgctcttt cgtgtgttgc
    10141 gccaagggcg actgcgccct catagtaact gtcaggaacg gctttaagtg cgctcacgct
    10201 catgcccacg atagtaggaa gtatcatgat accgaggata atggaaactg taagcacgct
    10261 gtttcctgtt ccgccgaaaa ggtttcttat gataggaact acaactacca tgccgaaaaa
    10321 gccgtataca atggacggta tgcctgcaag aagatttatt ggaggcatga gccacttgaa
    10381 aagcttttta ggacagaact ttgccataaa aatagctgtg agaagtccta taggcacacc
    10441 aatcattatt gctcctgctg tgcagtagag agagccgact atcataggga agataccata
    10501 tgaagcaggg acgtttctag gactccagtc tgtgccgaag aggaagtttc ccacgcctat
    10561 ttttgcgatt gcaggacagc ctttagcgaa catgaagata catattacta ttactgccgc
    10621 aactgaaacg caggcgcaaa ggaaaaacaa tgctttcatc gctttctcaa gcagttggga
    10681 actttgtttt gctgttggct tctgagcggc tgtattcgat gccaccgctg tgttttccgt
    10741 caacgtaatt atcacctctg aaaatttttc acttgttttt ttgctcagac aaatggggcg
    10801 acaatttgct gtgccgccca aagttttgtc ttgtgcaaat ctttatggag aattaacttg
    10861 atagcttatt tgctttcgtc agaccatgtt gtctttgagc ctgtatagat agcctttatc
    10921 tcgtccattg tgatatcttc aactgcgtta tccttattta cgataactgc gatagcgtcc
    10981 tgagcgatag ttacagactt gataccctgt gaaagctcgt cgtctgagat atctcttgaa
    11041 gccataccga tatcagatgt gccgttgata gcatccttga tacctgttga ggagtctgac
    11101 tgctgaacgt ctactgttac gtttgtgttt gccttctgat atgcttctgc aagcttttcc
    11161 ataaccggtg ttactgatga tgaaccgctg actactacct taccggaagc gttgtttgaa
    11221 gcgtaagctg ctgcgctctt gtcgatcttt atatagcctg ccttttcgat gatatcctga
    11281 ccatctgagc tgaggatata gttctcaaag tcctgagcgg cgtctgaaag tccgtcttta
    11341 acagcgatat tgaaaggtct tactatttta tagtcgccgc tttcgatagt tgctgttgaa
    11401 ggctcaacgc cctctacttt aagagccttt actgtatcgt tcagtgagcc aaatgagata
    11461 tagccgatag cgttcttatc tcctgacacc tgagtaagca caacgtctgt tgacttacag
    11521 ataagggcat ctttctttgt gttatcggtc ttgttgccgt tagagtcttt ttcctcgata
    11581 cctgtcagct cgatgaatgc gcctcttgtt cctgagccgt cctcacggga gataactgtg
    11641 atcttatctg agcttcctcc gcagcttgcg agcattgtgg ctgcaagtgc aactgatgta
    11701 aagatcgctg ccagttttga tgttcttgtt ttcatgatat aaaacctctt tcttatactt
    11761 gccctttggg ctttttgatt tgcacctttt ctctctgcac cctgtgtgca ggtcttcatg
    11821 ttatttctat tcaggtgata tacttatgat acatcagcat tgtaaaatca gtaaccgaag
    11881 tatagtaaag tcattgtaag atgtttgcaa aggaatttac acacaaaaac gggctttaca
    11941 ctgctatgca caatgtaaaa tacgtttgta taaacaaaaa aagtgcgttc tcacaaggaa
    12001 aacgcacttt gtaaaatcgg tgatattcta tttaaaatca gtagctctgt acaagtacaa
    12061 ctgagccgtc tgccttatta tatgcggcgc tgtagccgtg gccgtcttct gtaaagacgt
    12121 aatagtacat gaagcctgcg tcctcagttc tgtattcata tgaagacacc ttgccgtcta
    12181 tctgagctgt gatagcggcg acagcctttg cagaatctcc gctctcggct gatgtagttg
    12241 ttgtagtttc gtcaggcttt gagtctgctt tagttgtggt agttgttgta gtttctgtat
    12301 ctgaggcagt tgttgtggta gttgtcgttg tttctgtatc aggtgcagag gtttcggtgg
    12361 ctggtgttga gctttcgtca gccggctgtg aggtagtggt atttgtgtca gtagaaactg
    12421 cactgctctc ggtatcgacg aacactcctc ccttactgtc gtcatcagcg gaaacagttg
    12481 tttttggctt tgtggtagta gtagtcttat tatttgttcc gccaaagccg aagatcttat
    12541 agcctgcgac tatagcaaga atgaccacca caagtatacc cactgtgata gtggcagtaa
    12601 ttgcaaagag cttctttctg gaagcgtcat cgttatcctc tgtatcatca tcggaataat
    12661 cgttttcatc gtcaggataa ccgccattat cataattatc ctgataattc tcatagccgt
    12721 cattattatc atggctatca gctattggtg tggagaaaaa ctctgtaggc tcttccggct
    12781 gtttaggcac ggtaaaggct cttgttttct cttcatactc ctgtgcctcc tgctttgtga
    12841 acactcttgt tctgtcagca tcgtcagcag aggtgaaatt ctgctcctcc tgcttgctgt
    12901 cttccgtaac aggtgtgctt tcgtctataa gcaggttatg gcaatactta cagagtattg
    12961 cttcttcgga tatttcctta tggcaaaaag gacattcctt tgttttcatt tttactttcc
    13021 tcccgaatat tatctgttaa atataatgat aacacaaacc attttaaaaa tcaacaggtt
    13081 tcaccaaaat atcatcaaat tataccattc ttttaaaaaa ggctctttta tggacattat
    13141 ttttgatgtt ttcgtatgat atcacgatat aatttgttga agaatgacat ttggtttgct
    13201 tctaagcagg gcataattgt gcaagtatac aaagtgataa aaaacctatt gacaaaacag
    13261 gcattgggga gtataataat taagctgtct gatgacgcag acaaatgctt cacagaaaca
    13321 tctatgaagc tcaaggcaca caaagcttgg aacttttccg tggttggaga cttttcccca
    13381 ccgtgtgcaa cgtcaactac gaggcgtaac tcagtttggt agagtgcttg gtttgggacc
    13441 aagatgccgc aggttcaagt cctgtcgcct cgaccacttt attaaaaaaa tgaaaacaaa
    13501 ataaaaagtg cttgacaagt gcttggagat atgatataat agatatcgtc agttcgacag
    13561 atcaagcaat acaggctggt gtagctcagt tggtagagca gctgatttgt aatcagcagg
    13621 tcgggggtt
//
LOCUS       DAWEMR010000060        13584 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1889_length_13584_cov_11.000296,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000060 DAWEMR010000000
VERSION     DAWEMR010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13584)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1889_length_13584_cov_11.000296"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9138..9147
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9744..9753
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10869..10878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3738 a         3216 c         2539 g         4061 t
ORIGIN      
        1 ataccacaaa aaagactgct ttcctaccct tttaagggtg gttgcagtct tttttattgc
       61 gcgttttttt acagcccctt tatttatcag ttataaccgc aggcaagccg tttttcaaac
      121 tcagccgcag gcagaggctt gtcaaacaca aagccctgaa cagcgtcaca gccaacttct
      181 ctgagatagt caagctgctg ctcagtttca actccctcgg cgatagtttc cttgccaagc
      241 tttttaacca tttttataat gtaatagaac attataagat ctttatcctt gccctcataa
      301 tccttttcaa gaggaataaa ggacttgtct atctttatta cctgcagatc aacgttcttt
      361 atcatattga gcgatgaaaa gccagtacca aaatcgtcca tagatgtgac gataccatta
      421 gctctcagac cattcacgat ctctgtgata agctcaaaat tctgataatc ctcgctttca
      481 gtaagctcta tttctataag ctggtgatca agctcatatt tgtcgatcaa ggcaactata
      541 ttgcctatca tatttttttc ttccaggtgc ttcctcgaaa aattcactga tattctcaag
      601 ggttcaaaac ccatatctat gcgttttctc agccatttgc aaacttcttc aagcacatag
      661 tagtcaagct ttattatgct tccgtcattc gtaagaagcg gaacaaactc caccggtgct
      721 gataaccttc cctttctatg ccaacgtaca agggcttctg caccaaatat ctccttggtt
      781 tcgatgcgta cctttggctg ataatacacc tcgaactcac ctgccataag cccagccatg
      841 aagtttgaca gcactccctg aattttcatc agttcttgtt ttatattttc ggaatactcc
      901 attacatggc ctgcaccaat tcgtcttgca gcagcatatg caatgcttgc acgagccatt
      961 acttctcttg gatatcttat acctacagtt gacgaatatc cgatggttgc actaaaaata
     1021 aaatcctttg atactttgcc atcgtcatac cttatgttca taaagctgag catatcaata
     1081 tattctctga gcgattcttt ctttacaagc ataacgaaat tatcgccgcc aagtcttgtg
     1141 atcatctcac catctgaaag atttcggtat atagtctttg tataattacg caaaacaacg
     1201 tcgccctgtt cgtaatttaa taccttattt acatatttga agttatgtat attgaaaaag
     1261 attatactga aatcctctat cctgttttgc gctataagca taccggcgtg atttatcaaa
     1321 gcgtctaaag ttgctgcacc tgtttcgata tccgttttta taacatgagt aacaagctcc
     1381 tgcataatta ccctgttaaa ttggataaaa acagtgtttg cgattatctc tacgatattc
     1441 ctttgctcgt tatcaaaacc attggaatta ccagaataaa aggtcactgt tataaatccg
     1501 ccttcaaaca aaggaaaact tgcctcaaaa ggctcgtttt ccacgtcctt ctctctgtca
     1561 taaagcacca tgagcatatc tgcaccatcc tcacaaagct ttgttgcagg cattttcact
     1621 gcaagctccg ctctgacgat gtcaagcttg tctgccacag cagccacagc acttttcacc
     1681 ttggcactgc attcgtccat attctccgaa tcttcatata tcgttcggaa gaaaagcatc
     1741 aaatcattat caaaattcat ttttatcccc actaattttt tattcaatga tcacgttcag
     1801 gaaaacgttt tcaaaaatac ctgtacttac cttatctcat tttactactt tttataaaaa
     1861 aagtcaagca agcatagcaa aacgccgtcc ttttccgacg gcgttttatc gtcttagtag
     1921 ctatagctgc tctcacctac aaactccctt gcaagggaat ttacaggcac actttcgagc
     1981 atcaccggct ctatctcctc aagggctttt agcataggca aaaagcgttc aaaatcctta
     2041 tagctcctgc tctcacgtcg cagtgtatcc aaaagatagt tcttatacaa ggcaggctca
     2101 taagcgtgaa aagtatccac tgaaaactca gccctgtgct tgtatggcat tatatacttg
     2161 ttctcgccag cctcaacgct gtcgaacata tctagtgttt ccgctgcact tctgccacgg
     2221 aaaaatccgt cccttataag tctgcgcata agcctgatat agcaaggatg cagaagctca
     2281 ccgtctttca attcaagtct gcttcttacg ctgacgtaca tacatcttgc ataatcccca
     2341 gccgcacctg tgacctccgg gttaagggca tggataccct caaaaacaac tatgtcgttt
     2401 ttatttcgtc tgagtctttc tccatcacgc ctgcattgtt ctgaaaactc aaacttcggc
     2461 agaacgactt cctcaccact gtttatcttt tccatatgct cataaagaag ctttcgatct
     2521 attctgtcag gagattcata gtcaggcttg ccgtcctctc ctataacagc ggtgaagctg
     2581 tcagaaggca gaaaataatc gtccatagag atagtgtggg tgtgtatccc cctgccgtca
     2641 agtatttcct ctattttcaa cgctgttgtg gtcttgcccg cccctgacgg acctgccaaa
     2701 agcaatatag gtttctcctt ttgatgctcc tcgatgctgt ctgcaatggc aacgagcttt
     2761 tcattatatt ttctgtcgca ctcttcaaca aaaagctttt cgtcatctct gacctttctg
     2821 tttatagcac tgacgtgtat ctttttcatt gtgtggtttg ttcctttcgt atgcgttagg
     2881 caacaccaca cataaagcgt gcagtgttgc cctgtatctc atattatatg cacagcaaaa
     2941 gctaatcgtt ccactttgca cagatattat ttatggtatc agtgagcttt gcaaggtctt
     3001 tattcgtaag acctgagctt ttgttcacca aagccgtcag cctgtcaaga gcactgagca
     3061 gacgctgata gttaggcgac gcacctgttt tcttgactat ctttataggg gtcgtgtttt
     3121 tgaactcgcc gcttataagg tcaagctccg caccgctgta aggtgcaaag gcgttaagcc
     3181 cgaaatcgtt acgcaaatgc tccttatacg cctcagcaac tgaatcctcg ccgtggttga
     3241 caaagacttt cttaggcggc ttttcatagc tttttatcca cctgtccaga ccgtcaacgt
     3301 cagcatggcc gctcacaccg ggaagcacaa gtatctcagc cctgacaacg atttcctcgc
     3361 cgaaaagctt tgcagttttt gccccctcca caagccttct tccacgggta ttcatagcct
     3421 gatagcccac aaaaagtatg gtgttttcag gtttccacaa attgtgcttg aggtggtgtt
     3481 ttattctgcc tgcctcgcac ataccgcttg cagaaattat tatcttaggt gtgtcgataa
     3541 agtttatggc tcttgactcg tcacttgtga cagcgatatt aagtccgctg aaagtgatag
     3601 gatttatgcc cttgttcaca taggacattg cttcttcatc gaaacaggaa tttttgttct
     3661 tattgaatat ctgtgtggct tcgatagcaa gtggagaatc cacatacaca ggaaaatccc
     3721 catgacccgt caccatattc tccgccttga tatgccttat aaaatacagc agttcctgag
     3781 ttctgcccac tgcaaaggac ggtataacaa ggtttccacc cctgtcaaag gtcttttgaa
     3841 tgacctctgc aagactcttt gcatagtcct tcggcttatc atggcgacgg ttaccgtatg
     3901 tggactccat tacaacataa tctgcctccg ttatatactg cggatctctg ataagaggct
     3961 gatcaaggtt tcctatatct cccgaaaata cgatcttacg tccttcaccg ttctctgtga
     4021 gccatatctc aatacttgat gagccaagca ggtgacctgc gtcgataaag cgtaccttta
     4081 tgccctcgga tatctccgtc acctcactgt atgggtgtgg gcaaaggcag gcaatagccg
     4141 cctctgcgtc tgccatagta tagagtggcg acacctcgcc tgtaccctta cgtttgccct
     4201 ttctgcttct ccactctgct tcaaactcct gaatatgcgc tgaatctctc agcattatct
     4261 cacataaatt acaagtggcc tccgttgcgt gtatctgtcc agcaaagccg cctgccgcca
     4321 acagtggaag cagacctgaa tgatctatat gagcatgagt cagcaataca aaatctatat
     4381 ttccaggagg acaaggtatc ttcgcatttt caaaatagtc aacaccctgc tccattccga
     4441 aatccaccag aaatctcttt ccgcaggcct ctatatatgt acagcttccc gttacctcat
     4501 gtgttgcacc gataaaatta agtttcattg cccttatccc ctcgtttcgt ttatattctg
     4561 tgacagtatc tctatcacac ttacattata gcacattcca agcgtgattt caagcatttt
     4621 gcataaaaat atttgattat cggcaattat tttgtaaagt ctgaaagaac gtcaagtaat
     4681 aatgaggtaa ggaacagcat atagattact acagaaaaat ttaatggggt gtgaacaatg
     4741 aatggatccg aaaaggaacg atgtgtgctg cttggagaat atgttgcggc acacagagcc
     4801 acggtacgct gtgcggcaaa ggaatttggc ataagcaaat ccacagtaca taaggatctg
     4861 acagaacgtc tgccgaaaat ctctcctcag cttttcaaaa gcgtaaaaga ggtccttgag
     4921 gaaaacaaac gtgaaagaca tataagaggc ggaatagcga ccaagcataa atatgaacag
     4981 cagttcaaaa aggctgtaaa atgaaaaaaa ggacggtctg agccgtcctt tttatgttat
     5041 cagaattttc ttgcttccgc ccttttagaa ataagatctt tccatgcaga ggggtggaaa
     5101 agtatcagca gagggaaaag ctccaatctg cctgcaagca tatcaaacat gaaaacgaac
     5161 tttgaaaaaa cagagaagtt tgaaaagttg cctgctggac ctacctccgc aagaccaggt
     5221 cccatgttat tgatggttgt cagcacagca gtaaagtttg ttacaagatc tttaccatca
     5281 aatgccacca taaaaactga tgaagcaaaa attatcacga atgttacgat ataaacattt
     5341 atagaacgca ctatctcgtg ctcaacaggt ttgccgtcaa ggtttacctt gcgtatgctc
     5401 tttgggtgta tataggagtg aagctccttt atcattgtct tgaaaagcac gatcactctt
     5461 gataccttta tacctccgcc cgtactgcct gcgcaagctc ctatgaacat tataagtaca
     5521 agcaccgtcc ggcatatctg aggccacaga ttaaagtcag tgcttgaaaa gcccgtactt
     5581 gtaattagcg acgccacctg aaatactgat tttgtaagtg cgtcaaaaat gctaacacag
     5641 cggtcgagga tatttgcgaa tattatagct gtggcaatga ggattatagc aaaatagcac
     5701 cgtacttcct ccattttgaa tgccttttta aagtgcctgt atataatata atagtatgcg
     5761 ttaaaattca cgccaaacag tatcatgaaa atggcaacca cccactgcaa atatggcgaa
     5821 tagcttccta agctgtcgtt cttgactcca aaaccaccag tgcctgccgt tccaacggct
     5881 gtacaaatgc tgtcaaacac aggcattttg ccgataataa gaaaaataaa ttccaatatg
     5941 gtcatgccaa aatagattat ataaagtatc cttgcggtat atttcatctt tggaacaagc
     6001 ttgcccactg atggtccagg gctttcagcc ctcataagat ttatatttga gccaccgctc
     6061 agcggtatga tagcaagaag gaaaactaaa actcccatac cgcctatcca gtgcgtgaag
     6121 cttcgccaaa aaagtgcagt atgagaaagc gcctcaacat tggtaaggat cgaagcacca
     6181 gttgttgtaa agccggaaac tgtttcaaac atagcatccg tgaagcttgg tatctctccg
     6241 cttatccaaa aaggcataca cccaacaagg ctcataacta tccagctcaa agatgttgct
     6301 atacaaccct ctttgagata aaacacatag tttttaggct ttttccaagc cattaccatg
     6361 ccaaaagcaa ggcacacaat ggctgttcca agataataaa gtcccacgcc ttccccgtaa
     6421 accagcgaca ctatgcaagg cagcagcaaa aagcaggctt ccattcgcat aacgtttcct
     6481 aaaatatatc gaatgatcga gctgttcata tttcctccgc tatttcagaa tatcagtgat
     6541 atcgttaaat cccgtatgag tagtgacgac cattacagtg tcgcccctct ctatagtatc
     6601 ctgaccgctc gggataatga tcttgccgtt tcggtttatg aacgccacaa gcaaatgtct
     6661 ttttaaatca agagcctgaa gcggtgtacc tgtgacctct gactcctgat ccaccttgaa
     6721 ctcaatagct tctactctat gatcgaacaa atgatacagc gtttcgatat tgctatctcc
     6781 cgattcgttc ttcgcacgga cgtaggctat tatcgcctca gaggtgatat atcttggata
     6841 tactacgctt ccaaggtcca gtctgcttat aacgtccttg aagtttatcc tgtttatctt
     6901 tgtgatagtc ttagcatttg acacctgtct ggcatgaaga gtgagcaaga tattttcttc
     6961 gtctattccc gtgagagcca caaatgactc tgcatactct atgccctctt ccttcagaag
     7021 ttcctcgtca gtgccatcac cgtttattat tatcgccttt ggcaaaagca cgctgagatc
     7081 ctcacagcgc tgtctgtcat tctcgattat cttgacgttg atccccatgc caataagacg
     7141 ttttgcaaga taataagccg ccttgccacc gcctattatc attgtgttct tcacctgatt
     7201 tgtcttcaca ctgagatcct caaagaacgt tcttgaaaaa tttctttggg tgcaaaatga
     7261 gataacatca ttctttttca ttgtgaaatc gccagatgga atatatattt ccccttctct
     7321 ctcaacaacg catataagca ggcttgttgc cgttttacgg ctaagctcag cgatcgtagt
     7381 gcccacaaga ggattgccct caggtatctt atactttata agctccgcct gaccattcgc
     7441 aaaggtgttt acctcaagag ctgttggcaa acaaagtatt cttgacgctt ctcttgaagc
     7501 ctcaagctca ggatttatga ccattgcaag accaagcttc tctcttagat agactatctc
     7561 cttgctgtaa tcaggtgttc ttactcttgc tatagctgaa cacttcgcta ctctcttggc
     7621 aagtgtacaa caaagcaggt taagctcatc agactctgtg acagcaatta taaggtcggc
     7681 gttctctatg ccggcatcct gctgaacgct aaagcttgcg ccgtttccta cgatgcccat
     7741 aatgtcataa aggctactga gttcctgtat cttctttgca ttcttgtcga ctatcgtgat
     7801 atcgtggccc tctttcacaa gctgttcaac aagggtcgtg cctacttttc cgcagcccac
     7861 aatgataata ttcaaaccgc ctgcaggctt cttttgttta ctcatatttt tcctccaacc
     7921 gaatgacata ctgccattca cagcagttct ctgcacttct tctcttcatt aagcagagcc
     7981 tgatcatcaa aagcgtataa ttccatatca tataatgtat cactattctg ttaataatat
     8041 gttaaagtat attaaagata ttaataaaat ttacgacgat tgcctaactc atttttccaa
     8101 attagcgatt ttaaaaaaag ttataaaaaa agcacaaaaa aactgtgccg ccgatacata
     8161 ccgacagcac agctcaatta agaaagaatg atttggaaga attactctac atttttgagt
     8221 gcctcatcaa gaggtacttt ctttacacgt cttgtaagga taaatgctat caccgcatat
     8281 gaaacgatac cgagcaacgg cacttcaaca aatatttttg ctggcacata atatggcatc
     8341 cagcctgaat actgagcgaa agcaactttg aatatatact tcaaagtgac atttactata
     8401 ggaacagtca gcagcattga ggctataacg actatggtcg tagtcgttat ataaaggctg
     8461 tttatttccc tgttgttgta acccagtatc tttgtcatag aaatagactg gctgttcttt
     8521 tctattatga cctttgaaag aagataaaca attagcatga acattataac acccgctgtc
     8581 gcaaacatac ttatcataga acccatagac agtatgagct gtcgtgatgt ttttgtcagg
     8641 tcgtctttag ttatagttgt cgcaatatac agatcgtcta tatcctttat ctttgtattg
     8701 gagaaataac cattaaaata cccgtcctca agttcaaagg tctttatgaa gtcgctgtct
     8761 gtcagataaa ctgctatagt tgcaggatcg tcatagatac cctctacctt aaaattgtat
     8821 tttttatctc cgtaagactc tttcagcgtc acttcatcac cgacctctat gttatacttc
     8881 tgagaataac catcagagat aaacgctccg ccgtctttta tatctgcatg aacgaacttg
     8941 ctgttttctt taactccgta aattgttgcc gtttcctctt ttagcttgtc atcgacagtt
     9001 ttaagagagc ctgaacagta tttctctaca ccctctgttt tagtttcagc aggcattttc
     9061 agcacatact gatatttgct tatcatatta tcttctatta tatcctgata atgaagaagc
     9121 ataggcttca tgccaagnnn nnnnnnncaa ggccaaagaa gatgataaca ttggcaaaaa
     9181 gaatgccaac aaacactgta atatagttag gaatgttctg gaagatgact ctaagtctga
     9241 aacgctttaa tatgcctatc cttgtgttga gcttaaaagc ttttttcttc tgacgctttt
     9301 taagatcacg ccttaagaat ttaagcggtg aaagacttag tttatttaca agcattacaa
     9361 ggtttattac gaaaagtatt ataagaggaa ctactgtcgt ctttataaac gcatctgcgt
     9421 tccagatcgt tttgtatgtt ggcagactgt agcttccata gtacgctttg accgcaaatt
     9481 ccttaaagca ggtgtaaccc agtatgttgc ctacaatggc agatacaagc acgactatca
     9541 taggcgttgc aagataatgt cttacaagct cgcccttggt gtagcctgag gctctgagcg
     9601 tacctataac gtttgcttct tttgcaatag tgtttcccgt tgttatagca aaaacaaatg
     9661 cgattatgac cataacaaga taaagaaaaa ctgttatcat ggtgttatcg cccttgatat
     9721 cgtcgcctgt aaaatgtatc gccnnnnnnn nnnatcgcct gatttgagta ttccggaata
     9781 aactgagtga gcattgagat tccatttata tgcttcatga ccttctcagc catatcctga
     9841 gcttcctcgt cgtctgccgg ggttttatca tatttccatg agtagctata atgaagatca
     9901 gcctcaccat aatcgtcaaa aagggtatcg gtaacgatag ctacaccgaa cttcaaagcg
     9961 tcaaacatca tatcagacgg attagaatac agtgcgctgt aatctgaaag tgcaacaaga
    10021 cctgttatct taagcttctt gccgccgacc gtcaggctgt cgccgacttt aagcttgtta
    10081 ttatcagcat acattctgtc aatagcgatc tcgtcatcag cagaaggaag ctcaccgtcc
    10141 attatacata tcttgtcgat atccttacga tttttgaaaa gcctgatagt gctgtcaact
    10201 tcttttgttt ctttgtcaag ataaaaattt tcgtatacct ttatcttctc ctcaccgtcc
    10261 tctatcttct tgatagcatc atcatcggct ttttgaagaa gttcaaaatt tccgctttcg
    10321 atattgtatt tctcaaagct ttcgtcataa gcaacagaca tactgcctgc cgcaacaaga
    10381 aatcctgata ctgtgcctat catcattgtg atgaacaaaa atagaacgat gtattttcct
    10441 atttcgccct taagctctct gggcaaacgc ttgataagag ggtttttcat gctgaccctc
    10501 cttaccaatc aagctcttcg gctgtgagct tagtttcatt aagataattc tttcttatca
    10561 taccatctct aagctttaca actctgtcag ccatttgctt gatagcgtca ttatgtgtta
    10621 ccattatgat agtgttgcca tacttcttgt ttacgtcctc tataagctgc aatatctcct
    10681 tggaggtatt gtagtcaagt gcacctgtag gctcgtcgca gagcaggata tcagggttct
    10741 tgacgatggc tctgccaata gcagttctct gctgctgacc acccgaaagc tgattaggaa
    10801 gcttgtgacg atgctcgtaa agcccaaggg ttttaagcag gtcgtcaaca tcaagagggt
    10861 tgtcgctann nnnnnnnntg cgcctacctc gatattctcc ttgatattca ggttaggtat
    10921 aaggttgtac atctggaaga tataacccaa gtgctttctt ctgtaaaggg tcaatgcctt
    10981 ttcgttcatg tcggctgttt tttcgccgtt tattgagata tacccctcgt ctgcggcgtc
    11041 aattccgcct ataatgttca gaagtgttga tttacctgag cctgacggtc cgagaagcac
    11101 gcagaactct cctttttcta tttcgatatc aatgccctta agcacttcga ctctgctctc
    11161 gccctcgcca aaatgctttt ttatgttctt tatttcaaga aacaccttca ttcccctttc
    11221 taactattta ttttaataat gtatctttgc cgtttttaaa cgtcatatta tacatatttg
    11281 tatgccataa cttacttcac attgtggtta attacaacta actgatatat ctgataaatc
    11341 ccgccgaaaa aggcgggacc taacgatata aatattactg atttagccgt ttgtcgtgct
    11401 ttgcaaagca aagcttacaa gcttgtcgag agcgtcatca gatatctgag aaacaatagc
    11461 caccgagtca ttctcggcat tttttctctc aacgcccaca cggttttcaa gaaaatcctg
    11521 aagtatcaca cttggagtat aaagctcatt ggctgccttt atgccctcag atgtcagtgt
    11581 gatctcacgg taaggctctt tgattatata gccctcgtct ataagcttct gagtcatctt
    11641 gacggtgctt gcctttgtca cgcccagaac agaagctacc tctgtggact tgacaggtct
    11701 gccgttctga cccagtctgt atatggcaaa taaatacctt ttctgtgatt tagtcagcat
    11761 tcagatgacc tccccgaagt ttattactgt tttatcatga tctgtgacag cgagaagaat
    11821 ttggacaaca gccgcagctg ccaccgcaag agcttttgcc ctgtttctta tcctttacga
    11881 gcttgtaaat tatagctgcc acgatagcag caagaatcac tgcaaccaga atcgtaccgc
    11941 ctactgtgtc cataattatc ctcccaactg tttatgcaag atcagtgatg atatttgctt
    12001 tttattatgc gtttgcgcct accttaacgt ccttttcaag ctttgtgctt tccttataag
    12061 gtctaacgag catatagatg ataagagcga taagagctat tgcaaagata agaccgataa
    12121 cattcacctt accagcgaag aggttgccaa tctgatttac gataagtgag ataacataag
    12181 caaaaccaca ctcgtagcca atagcgaacc agaaccactt tctgctgttc atctcacgct
    12241 tgatagctcc cattgctgcg aaacaaggtg cgcagaggag gttgaagata aggaatgaca
    12301 tacctgtgat gccagtgaat gcagcaccga tcgcagagta aactgaaccg tctccgccgc
    12361 cgtaaaggat acccatcgta ccaacgatgt tctccttagc aacaagacct gtaacagctg
    12421 caacagttgc ctgccagttg ccccatccaa gaggtgcgaa gatccagcag atagccttac
    12481 caatgtgagc aaggatactg tattccatct ggtcttctgt gagcataccg aacgaaccgt
    12541 ctacccagcc gaagtatgaa gtgaaccata cgaagatagt tgacagtgtg atgatagtac
    12601 cagccttctt gatgaatgac cagccacgct cccacattga tctgagtacg ttgcctactg
    12661 tagggatatg gtaagcagga agctccataa cgaatggtgc aggatctcct gcgaacatct
    12721 ttgtcttttt cagcatgata cctgaaatga tgatagctgc gataccaatg aagtatgcgc
    12781 ctgttgctac ccaagcagag ccgccgaaga tagcacctgc gatcattgcg atgaatggtg
    12841 tcttagctcc gcaaggaata aatgttgttg tcatgacagt catacgtctg tcacgctcgt
    12901 tctcgatagt tcttgaagcc atgataccag ggataccgca acctgtacca ataaggatcg
    12961 gaataaatga cttgcctgaa aggccgaact ttctgaagat cctgtccatt acgaatgcga
    13021 tacgagccat gtaaccgcaa gcctcaagga tagcaaggag aatgaagagt acgagcatct
    13081 gtggtacaaa tccgagtacc gcaccaacac cggcaacgat accgtcaagg ataagaccct
    13141 tgagccaatc tgcgcagttt actgcgtcaa gaccgctttc gataagtaca gggataccag
    13201 gtacccatac gccgtaatct gctgggtctg gatcagcgaa gctgtatttc tcagcggctg
    13261 caactgcagc tttcagatca gcatacttag ctgtttttgt tgtttcttca agtgtttcct
    13321 cgtcctctac tgtataatca gcagttgcgg acttatctac tgtagcaagc aggttgtctg
    13381 ctgttgcctt agcagcgtca acatcaaagt catcagcttc gttgtcgata gcctcgccgc
    13441 cctgctgctc gataaatgcg ttgattatgt caagcgagct gccatattcg tcagcagcgt
    13501 cctcagcgtc agatgaaccg ataccgaaca gatgccaacc gtcaccaaag agtccgtcgt
    13561 ttgcccagtc agttgcggct gaac
//
LOCUS       DAWEMR010000061        13573 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1893_length_13573_cov_8.469818, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000061 DAWEMR010000000
VERSION     DAWEMR010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13573)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13573)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13573
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1893_length_13573_cov_8.469818"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1008..1017
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1435..1444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7875..7884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4039 a         2578 c         3058 g         3868 t
ORIGIN      
        1 ttgaccgcaa gttctccgat atattttatc atatgagaga aatcattgat ttgcatttcc
       61 tggcattttt ctgttaacct gtcacaaagc tgtaagtctg taatatttct gctgtcggca
      121 atgcctataa gctttaaata tttgagcata tagcgatata ccgccacagt gcgtgatcta
      181 ttgtccccat tacttagttc cgaacgcatt ttctttacct taatatctct atggactaca
      241 atgccgaagc ctataagcag aagtccgccc aaagtgatga aaattcctgc aaatgagcca
      301 tggtcgctgt tgttttctgt tattcttgat gaaacagaag ttttcccagc gtctgacatc
      361 actccggcac catgtccgtt gcttgcaaaa tttgttttct ttgttgttga agcttttgaa
      421 ctgtttgcag gctttttagt aaccactgaa gttgtgccac cctgaccgcc attttgtgtg
      481 gtagtcgtag gcttgctttc tgtgttttta gttgttgtga cttccttagg gtctttctct
      541 tgttgtgtaa gattaggatt atcattatca taacctggtg tgaattctgc aggataccaa
      601 cctgcattgt ctataaatac ctcagcccat gcgtgcgcac atttgtcctt tacacttacc
      661 tcgtatgttg aatcatcttc aggttcaccg agctgtgaat taagcaccac atacccctct
      721 acatatcttg acggatagcc aaacattctt aaaagctctg cacccgctgt tgcgaaatat
      781 gaacaataac cctccttttg tgtacctaaa aaataatcta tgaaatcttc accttttgga
      841 gtaacacccg gttcaagggt ataagtgaag ttatcagaga aatagttttt tattgcagtg
      901 cttatttctt cataagtcca atcacccttg tggtaaatat ccacgccaag atattcgttg
      961 agtatctctt tatatgcctt atcaagctca tctgactttg aaacgtcnnn nnnnnnncgt
     1021 caacacctct gtccttatta acagacaatg caggcacttc tgtggctata gcctcaggga
     1081 gggcttcaag cctttcttct ataagagagg ggtcaaaata gtaaagagaa tactttgttg
     1141 aacttaattt gacatatgac tcagtggtag gacgcatttt tttatcgtca ctgtttccat
     1201 cacttgaata taaagaagca tatggtgcat acacaaattt cttgcttgcg ccgagaacag
     1261 atacagaaat ctgagcaggg gtcagatcag cgtatttgcg ttggataagg tcataatcca
     1321 gatcctgcac ccatatcttg ccttgctcaa aatcatcaga gaaggtgtct tcattgccat
     1381 tcacgtcaac aggagtccag ctattgtcgt tataaacgcc tgctacatat ccacnnnnnn
     1441 nnnngtatta gagaattttg gagtgacaac ttttagtgct gttgagccgt tgaagctaat
     1501 gcctgctgtc ttgccaagaa cgcctccgtt tgtgccacca acggttttta taccaaacag
     1561 atcaaatcca ccgtcataat ctgaaaaagc attcctcagg tcagtgagag agaaatttga
     1621 cactgctgta gaaatatcat gtctgtattt tgcaaatgac tcaggacgtg taaagcctgt
     1681 tattgctgag aaaagcatta taactacaag taccaacgag cttaaaatag ccgtgaattt
     1741 catgaatacg ttatagaaac aagctttttg cttttcagat gtaaaataaa acgtgcgttt
     1801 tcgctcatgc accgcaaagg tgttttttct tcctgccttg ttagttgtgt ggtttattat
     1861 gtgcagtgca agcactgtca cccagcatat aaacagacca ataacagtcc acattgacgg
     1921 ctcccagccc caatacattc caagctcgaa aacagggaat gtaatgatga acagcagtgg
     1981 aaaatctatc ctataaaggc aggctgctgc cgcaccaagg caaacaagag ttataaacaa
     2041 tacgaaaaaa tttgacgcaa gatatgggta gtcgatagta gaaatcccac taagctgaac
     2101 accaagagtt gagttaggtt gatttgccct tgaaagatat ctgtctataa caacgaacaa
     2161 gccgtatttt attgtagata tcctgctcac aaggtaaatt acatgaagta tcatcacacc
     2221 aagtgcagaa aacttaatta tcttgtgtga cgatttcatc agaccaaaca caagtgaaag
     2281 catggcggag taatatatga tcgtggtctt gtatatttcc atatcaagga atgatactgc
     2341 cataaacgct gtgcatatgg tcgccacaaa ggtcaaaagt atctgaaacc atagaaaaga
     2401 gcctgaatta tgctcacgct cacaattcat aaggatatct ccgtcaatgc agataccatt
     2461 gctgctgata aatcctatat ctttgttttt gctttttatt cttttcatgt tgtcaccttt
     2521 atagacatat ttcctgtatg ctttcggcaa cgtgttttgc agtaacgttc acgacctctg
     2581 caaactcatc gtttataacg ttgtctttct ttttgtttgt gataagcata taggaatagc
     2641 ggaacgccag atcgttatcg ttcataacgc cgctgagatt gctgttatat gcgggagaaa
     2701 aatacatcaa gtgaccgcat ttatatctct caggctcatt gatgtaattt atcatagaca
     2761 ggtcaggctc atcataaaca gaagcctgca acagcagact tataagcagc cttgcacttt
     2821 cttcgtctgt aacgttcact acttcgctca aatctttctt tttatcatac cacactacct
     2881 tatgaggtgt gtcattttct atgagataat aggatataga cgctatagcc tctacaagag
     2941 tatcatatac gagcatataa tcgtctgtat ttgtatcaag gtggagatcc gccataagca
     3001 cgatagaatt tgatattggc agagagtaat ccttgaccat ggtcttatcc tgctttgcag
     3061 taagcttcca gtgtatcctg tttattttgt caccatcatg atattcgtgt atatcaaaaa
     3121 tctccgaggg atcatcgccc tttgaggtct tggaataatc atcggtttca agacccattt
     3181 cagcataatt cgcaatgttg ttttcaatag gtatatagtc aggaactata gtgaacgtac
     3241 tttcgccaaa aagtctggaa gcagcatcgg tgcttactct tatcttgaaa agcttcagca
     3301 tatccacaat gcgacagcgt tttatgttca tgcgcactgt gccataatgt tttgcagaaa
     3361 cgtgcaaagt aagatactga acatcatcag gataaagggg agtatttatc ttcattgtat
     3421 ttttgtcgcc gtcaagagtg ttgtaatatt ctatctctat aatcatattg gcaacaggca
     3481 actttgagcg gttttgcagt ctcagcacaa caggtatctt tgacgatctg cctgcggttt
     3541 tctgagtgtt cacaaaacca acgcttattt tctttgacat ttttttgagc ataacgaata
     3601 agattatagg cataacaagt aaaaatgcaa agagataaaa agagaagcta cccacatata
     3661 atatatagaa aaagatagag gcgataaata atattatata taaaaccagc atttgctcct
     3721 ccgttacttg ctaactcttg gaacctgaac tgaggaaata actcctttga gtatctgttc
     3781 tcctgtgaag ccatttatct ttgcctttga gctgaacaca agtctgtgaa gaacaacgtc
     3841 cttgaagctg tatataacgt catcggggat gacataatct ctgcccttaa gaagcgctgt
     3901 cgcctttgtc attcttagta gtgcgatagt tcctctaggt gaaagaccta actttatata
     3961 ttcattgttt ctggttgcat ttgcaagcat aacgatatac tggaaaacct gatctgaaac
     4021 ataaatattt tccacaactt tccttgcctg aaggatatcc tccgcagaca ccacaggcct
     4081 tacattatcc acagggacga gattttgctt ggattttagc attgcgactt cgctttcaag
     4141 attaggataa cccattgtga gccttacgat aaatctgtcc atttgcgatt cagggagcat
     4201 ttgcgtgcct atagagccaa ccgggttttg cgttgcgata acgatataag ggtcaggcat
     4261 tttatgtgtg ataccatcta cagttacctt gccctcctcc attacttcaa gaagcgcaga
     4321 ctgcgtctta cttgaggttc tgtttatctc gtctgcaaga aaaagatttg tgagcgctgc
     4381 accctgttta tattcaaact tgtgtgtttc tttatttagt atggtaaaac ctgtcacgtc
     4441 tgttggcaaa acatcagggg tgaattgtag tcttttatag tcaagtgaaa gcgcctttga
     4501 aagagccacg gcaagagtgg tctttcctac tcctggaata tcctcaataa ggatatgacc
     4561 gcctgcaagt atggacagca agacttttat gattatctcg tccttgccga taatgacctt
     4621 tcttacctca tttatcacgc tttgtgacat attcagtact tttacctgtt cttcagttaa
     4681 gctgttgttc ataattttct cctttgtttt gtatgtttct tttatcttca caatatttgt
     4741 gcatattgca ttaacattat atcacaaata caacagtatt tcaagcattt tgcgtgataa
     4801 ttaaatacaa taatggatat ctgcgtatgt aggagaatac aaatatcttg tactaaaatg
     4861 caggctcaca gcatagttat ttcataggac aagtcaaaag gagcgtgaaa aaatgataag
     4921 agaagtcagc aagataaaat acgtttacga actgctcacc cctcgcataa ggggagcgat
     4981 tatgtcagtg ccagaaatag agcgtgacag gatacaggaa ataaggcttc gcagaggtag
     5041 acggctgtct gttaccatat tctcgaaaga gtatttcgtt aatgataacg gcagactaat
     5101 gaacaatatc ggggatagcg ttcaagttac cacagaggat attgaaacta tctatcagcg
     5161 agcatttcag aattctcttc acagctttca ccgtgagata gcaagaggct acataacggt
     5221 ttcaggaggg tgcagagtag gcttctgcgg aactgccgtt cttgatcctg caaggagtta
     5281 tgccgtggag tcggtaaaaa atatatcttc tcttaatata aggatagctc gtgaaatgat
     5341 aggcagctca gacgagatat atgcaaaatg cttttctgac aaaccaatga gcctgcttat
     5401 tgcttcaccg ccctcgggag gaaaaactac agtgcttcgt gatcttgcaa gaaagcttgg
     5461 agaacgctgg cgtgttgcgc ttatagacga gcgaaacgag tttgccgcaa cagtggcagg
     5521 tgagccgcaa aatcttatag gtgcaatgac ggacgtgttt aattcctaca acaaatatga
     5581 gggtataatg acggctgtca aggttatgtc accgcaaata ctcatatgcg acgagatagg
     5641 ttcttctgag gacaacgaag ctttgcagta tgcgctcaac tcaggagtga agcttattgc
     5701 ttcatgccat gcttcaagcc ttgatgagct taaaaagcgg cgttatatct caaaactcat
     5761 aaaagacaag gctttcgacg cacttgcggt actaggcaca ggaacaatgt gtggcaggct
     5821 ggtatcattt acaaaaacag gagcatgaaa tgataaagct aataggctgt gtgctgatca
     5881 tatccgcctg cacacaaatg ggctgtgcaa tggcgacgga caagatacgg caaatgaaat
     5941 atctcaagct tctcagacgg ttgatatttg aaacaagggc aatggttgag ggtggaatga
     6001 ctttcgggga aattatctta aagcttagct tgcagaagga ttattctgac tttacatttt
     6061 tgtcgcaaga tacctcttcg cctgatgttc gcagaagaat aaccgaggat atatataagt
     6121 caaagctttt tgacaatgaa atcaggcaga ccgccataga tttttttgaa aaactcggta
     6181 caacagacac aaatggacag cttgcataca tttccatgag ccttgccgca cttgacgagc
     6241 agataagcag actttcagag aatttcacct cacaggtgcg gctatgcagg gctttgggcg
     6301 ttctcagcgg ggcttttatc gctattctgc taatatagcc atagcaaagg ataaccataa
     6361 tgaagggaac aaaattttat ggacatagat cttattttca aaatcgctgc cataggaatt
     6421 atcgttgccg tgttaaatct cttgctgaaa cgagcggagc gtgacgagca ggcaatgatg
     6481 acaactcttg cagggcttat tgtcgttctg cttatgatag taaggcagat cggaaatcta
     6541 tttgaaacga taaaagaagt gtttggattg tattaatgaa aatatttaca atagcgtgct
     6601 tttgtatttt ctcaaccata gtgtgcaaag ccattgagaa agacagtcgt gaaataaaat
     6661 atgcggctgt gcttgcggcg gcggcactgg tgctttttca ttcggtggca tttgtgaaag
     6721 atcttatctc tgtatttgag gaccttttca ttctgtcagg aattgacgat atgtatctta
     6781 ccatactgtt caagtcactt ggtatatgct atatcgtaca gcttgcatct gattattgta
     6841 aggactgcgg agaaaacgca cttgcgtcac aggtcacttt ggcaggaaag cttgcaatgc
     6901 tttcggtatc gctgccgctt ttcaaagcgt ttgtagaaat agtaaaagca ttgcttatat
     6961 aggattaaca tatgaaaaaa ataattgcca ctgtcatcat agtccttgcg gtcatgttta
     7021 caccacaggg agtatatgca gacagtgagg aacagaacaa tgaatattat gacaggctca
     7081 taagcgaaat aaacgaggga ctttcttttg agggtgatga agatgtaaaa actgtacttg
     7141 aagataataa catcactttg gaagatccgt cctctgccgc aaatataagt gtctctgatg
     7201 tgataaagca tatatttgaa agctttaccg ctgctttgcg aagccctctt ataatgctcg
     7261 ggaaaataac agccgccact atgctttgcg tgttggtaag gtcaatgtca gccgagggta
     7321 cagtggacaa ggtattcacg ctcatatgtg ttcttaccac agtgcttgtt ataagcgata
     7381 ctgtcaccga cagcctttat tctgtaaaga cctccatggg acaaatgaac agctttatga
     7441 tgtcctacat acccatattt tcaagcgtgg cgacagcagg cggtagtgcg gcggcaggag
     7501 caggctatta cagcgttatg ctgataatat gtgaaagcgc aggtatattg gcagatacga
     7561 tacttgtgcc gtttttgtcg gcagtgcttg cagtgaccct tgtttctgca ataaacccat
     7621 ctcttgacct tggaagcctt gcagagtcgg tgaaaaagct ggtcatatgg gtgcttggca
     7681 ttgtgatgac cctttttact gggctgttat ctattcaaag ctttgcagga gcggcggctg
     7741 ataatctctc cgcaagagcc gttaaatttg cggcttcaag ctttatacct gtcataggcg
     7801 gctcggtctc tgaagcttat tcagcggtaa agggaagtat aggcgtaata agaacaagcg
     7861 tcggcgtgat aggcnnnnnn nnnnctgtga tagttgcgaa acctttgctt acgcttcttg
     7921 cagtaaaact tgcagtgtgg ataggcgcta ccataaacga tatttttggc ataagcaaca
     7981 gtggtggact tcttaaaagc ataaactcgg ttttatcaat tggtgtgagc atccttgcgg
     8041 cttattctat aatgttcgtt atttccacat cagttgtaat aatgacagcc atgaacacag
     8101 gagcttaaaa tgaatacagt taaatcagtg atatttattg cctgcattat aggcgtgata
     8161 acttctatga cagatattgc cacgcctgag ggaactttga aaaaacaact tagatcggtg
     8221 cttgctgtga tacttttgct tgcggtcata acgcctgtaa tgggacatgg cttcaaagtc
     8281 accctaagcg attatacaga gcttgcagat ctgcctgagt ttgatagcgt ttcaacagcg
     8341 acgaaacttt attatctgtc cgaatccgag agcaggcttg aagaatattt tttggataag
     8401 ctgaataaga atggcataga aaacatcaga gtggacataa ccacagatat aaatgaatat
     8461 aatgaaataa agataacagc ggtaagggta tatctgacta aaacaagcga cagaggactt
     8521 gttgaaaagc ttattgcaga agatctgcct gatactcaag ttatcgtgca ggaggaataa
     8581 acgttgctga aaagtaaagt tgaggggaaa ataaaggctc ttaaaaagct tcctatgaaa
     8641 acgaagatag tgatagccgt aggtcttgca gggatattac ttatatttgt ttctgagatg
     8701 tttcctgcta aaaatacggc agagagcaag agcgttccca cggaaagcgt tgccactgat
     8761 gatacggaca gctacaaaaa gcagatagaa aaagagctta aagatgttct ttctcaagtc
     8821 aggggagttg gcgagtgcga ggtaatggtg acagttgagg gaactaccga atatgtttat
     8881 gccgaaaatc ttaccaaaag cacagacaat aatggtgaca gaacaagcga caggtatgaa
     8941 aacgagatag taataactga taatgacggc aaaaaggagg ctttggtcag aaaaataata
     9001 aaaccgcaga tatgcggcgt tgtcattgtt tgcgaaggag gtggagatat aaaagtaaac
     9061 gaacgtgtgc ttaaagcggt ttcgacagta ctaggtatat caagcagtaa gatctgcgtt
     9121 gagggcagaa aataacagaa agaggtaata aagatgaaaa aaccgaccat gataataggc
     9181 aaaaagcaga taatccttgc aggaatgacg ctgatactcg gtcttgcagt atacataaat
     9241 tatgcagtat cgacctcaga cggcaagata aaggcgacgg acgttgtgaa aagtcaaagc
     9301 gtgaattatg gtgacgcaca gctcgtaagc actgacagta cttcagatta ttttgcacag
     9361 gcgagaatag acagaatgaa ttctagggac gaggcggtag aaacattaaa gaatataatg
     9421 aacggcggcg attccacaga ggaagaaaaa aaagtcgctg aagaagaagc ttcagccatg
     9481 acagggctca tggagtcgga gagcaaggta gagacactcg tgaaagcagc aggctttgag
     9541 gactgtgttg tttatcttga cggtgacaac gcaaatatcg ttgtaaagac cgacagcgac
     9601 gggcttgttg caagcgaagc ggcacagata aaagatatcc tccttagcga agtttcagtt
     9661 gcaaatgaaa acatcagaat ttatgatgta gaataaaatt tgtcagcaaa taaaaaaagt
     9721 ccttcaaaat acttgtaacc tattgatttt tatgatataa tggttgtaag tatagattga
     9781 aggatttctt ttttatatct aaaaagggag gcttggataa atatgaatga tgtttcaaag
     9841 aaagtagaaa aaagcctgca cataagtcag gacgttatag cgcagatcgt tacaaactct
     9901 gttaatgaga tagagggcgt ttatggtatc gctcctgtta tgaagactcc acgtcagctc
     9961 tggtttaggc aggaaaattt cggcaatatc agaataggcc ttgttgacga tgtgctgtca
    10021 gtgtctatag gtataatact ttgccgtgga gctaaggcgg tttccgttgc cgaggagata
    10081 caggataaag taaaaagtgc agttcagaat atgcttggac ttacagttgc aaaggtaaat
    10141 gtgacagtat gcggagttga cctttcggaa aaggaatagg attcagacaa atataagatg
    10201 aaggaagtgt ttttgtgcca attaaaaggc gtgaaataag agaatcagca tttttgctga
    10261 tatttgagaa aatgtttaga caggacgagg agcttgacga tattttcatg tctgccatgg
    10321 aagtgaacga gatcatcgta aatgatgaag ttaaaaagct cgtggaaaat gtttgcgaaa
    10381 agcaggacga gatcgacagc ataatcagca agttcagcaa caagaggact gttgaaagaa
    10441 tacctaagat aaatcttgca atactcagac ttgctattta cgaggcaata tatgaggaga
    10501 acgttcctgt gaacgttgcc ataagcgagg ctgtggctct tgcaggaaaa tatgccctcg
    10561 agcctgacgt ttcatttgta aacggtgtgc ttggctcatt tgcgaaggaa caagaggaaa
    10621 agcagtgagt tttgtactcg gaatagacac aagcaattat accacttcat gtgcactgct
    10681 tgatactgcg gatatgagcg tcaggtcatg caaaaaactt cttcctgtca agcagggaga
    10741 gcgaggacta agacaatccg acgctgtgtt tcaccatact aaacagcttc cagaacttat
    10801 gaaacagctt tttgacaaaa ggtatgactt aagtgcagtt gggtattctt atgcaccaag
    10861 gtgtgcagag gggtcatata tgccttgctt tcttgtcgga gaaaatgttg cacaagctgt
    10921 gtcacttgcc tgtggagctg accttgaaaa gacttcgcat caggtgggac atatacttgc
    10981 ggcactttat tcctgcggaa agctggatat gctcagttct gataagcctt ttgcggcttt
    11041 tcacgtcagc ggtggcacta cagatcttct tttgtgcgag cctgacaagg ctaagctgat
    11101 aaatataact cagatcggcg gaagccgtga tcttaaaggc ggacaggcca tagacagaac
    11161 aggcgtaagg cttgggctgt cttttccatg tggtaaagag cttgaaaggc ttgcgtctga
    11221 aagcgaacat aaaataaagc caaaagcatc agttgacggg ctttattgtt cactttctgg
    11281 aatagaaaat cagtgcatga agcttataga cgagagctgt tcggtggctg atgttgcggc
    11341 atactgtatt gattgtgtgt gccttgcggt ggagaaaatg acagttaatc ttcgccatga
    11401 gtataaggat atacctatta tatatgcagg cggtgtgatg tctaatcgct ttataaaaag
    11461 caagcttgaa aagtacggca gctttgctga gcctgagttt tcaagtgata acgcagtggg
    11521 cgttgcggtc tatgctgcga tcaggaaagg gaagatcaaa atatgagttc agttatcact
    11581 gtttctcagc taaacagata tcttgcttca aaactgcggt ctgaccttaa gctgaaaggc
    11641 attgccgtca agggcgagat atccaatttt aatatacatt acaaatcagg tcatgcctat
    11701 tttacagtaa aggacagcag ttctgcactt aaagctgtta tgttttcaag cagtgtttca
    11761 aggcttaaat tcgtgcctga ggacggcatg agcgtgcttg ttatgggcaa tatagaggtc
    11821 tatgagcgtg acggcgttta tcagataata gctacggaga tcgcacctct tggtgtgggc
    11881 gttatgcacg ttcagctcga acagaccaag aaaaagcttg aaaaaatggg cgtatttgat
    11941 gtgaacaaaa aaaagaaaat accccttgtt ccaaagaaga tagctgttgt tacgtcgctt
    12001 actgcggcgg ctttgcagga tataatccat atagctgaaa ggcgttatcc tgtgtgcaat
    12061 atcgaagttt atcctgcgca ggtgcagggt acggcggcag cacaaactat atgcaaagca
    12121 ctttcggcgg ctgataaaag cggtgcagat accataattt tagctcgtgg cggtggctct
    12181 agcgaagatc ttatggtgtt caatgacgag gctgtggcgc tggctgttta cgcctgcaat
    12241 acacctgtga tatcggctgt aggtcacgaa actgacacca cccttgcaga ctatgccgca
    12301 gatatgcgtg cgccaactcc gtcagcggcg gctgagattg ctacccctga caaggcggat
    12361 atacttaatg ctgtgaggct tttaaggcaa aagcttgaca aggctatgga gcttaaactt
    12421 gataaggctg tgtcacagat agaaagctgt gataagcttt tgaaattata ttctcctgaa
    12481 aagagattag agcagagtga aaaagcagtg tgcgaccttg aaaaaaagct gtcacagatc
    12541 atggaacgca ggcttgaaaa gctgtcagtt aagcttgacg gctatgtttc ttctctgaat
    12601 atgcttagtc cgttcaatat tcttggcagg ggatatgcta tcgttcagaa agacgaaagc
    12661 gttgtcgaca gtgtatcaat gctgaacgtt ggcgacgagg taaacataaa gttgtcagac
    12721 ggcagtgtat ctgctaaaat aacatttgtc aaatgaaagg ctgtttaaaa tgacttttga
    12781 agaaaatctt acaagactta atgatatagt tggtcagctt gaaaatgaca agcttcctct
    12841 tgagaaagct ttggcacttt ataaagaggg gatagatctg tcggtcgact gcaaaaaaag
    12901 ccttgaaagt gcaaagcttt ccgtaaaggc tatgaacgga gataatagcg atgaatgata
    12961 tgaacaaagc cacacttgat tattatgtgg agcttataga taataaactg ctggagctta
    13021 cccgctcaaa gcctgagctt cagggtcagg tagttgaggc tatggagtat tctttgcagg
    13081 cagggggcaa aaggctcaga cctgtgctta tgcttgaatt ttgccgtatg tgcggtgagc
    13141 gtcagcctga gaagtatgca gagatagctt gttctattga acttatacac actttttctc
    13201 ttatacatga cgatctgcct tgtatggacg atgatgatta cagacgtgga aagccctcat
    13261 gtcataaagc ttttcctgag aatatcgcac tacttgcagg cgatgcactg aacactcttg
    13321 cttttgaagt aattgccaac agtgccatga acggcgttat atctgccgat aaggcagtca
    13381 tgcttatttc tgttctggca agggcggttg gtactgacgg catgataggc ggtcaggtga
    13441 tagaccttaa atatgaaaac aaagaaatag atgttgatac actgaatatt ttgcagacct
    13501 gcaaaacagg cgcacttatc gaggctgcct gcgtaatggg cgttattctc ggcgataagc
    13561 ttgaggctat ccc
//
LOCUS       DAWEMR010000062        13569 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1894_length_13569_cov_12.119728,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000062 DAWEMR010000000
VERSION     DAWEMR010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13569)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13569)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13569
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1894_length_13569_cov_12.119728"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3675 a         2746 c         3539 g         3609 t
ORIGIN      
        1 cctgaagcgt tcctgcacct gctacacagc cgccaggaca agccataccc tcgagaagat
       61 agccgtcata tttgcctgct ttcgcaagtg tgagaagctt gcggcagtct ttaagtccct
      121 gcgctgatac taccttgacc tcacgctctg ggtcaagctt gtggataacg tctgctactg
      181 cctttgctac gccgccgctt actgcaaagc cacgtccgtc acctgtaccc tcgcaaagat
      241 ctactttttc ttcatctgta atatcttcaa atgttacgcc cttggcattg aacattccca
      301 tgagttcctc aaaggtaagc acaaagtcta cgtcgctgcg gatagtacgt cttgaagctt
      361 caagcttctt tgccgcacat ggtccgataa atgcgataca agcgtcagga tcgtcctttt
      421 tgagcattct tgctgtaagt accattgggg tcaaagccat tgaaatgtat ggggcaaggg
      481 tagggaaaag cttctttgcc attactgacc atgctggaca gcatgatgtt gccataaatg
      541 gctgatgttc aggaacttct ctcatgaagt cctttgcttc ctcaatggaa caaaggtctg
      601 cgcctattgc tacctcgacc acgtctgcaa agccaagctt cttcattgcc gctgtgagct
      661 tttcagggct tgcctttggt ccgaactgac ctacaaatgc aggtgctacg atagcgatga
      721 ccttcttctt tttcttcatt gctgttataa gctggaatat ctgtcccttg tctacgatag
      781 cgccgaatgg acagtttaca agacacattc cgcaggatac gcacttgtca taatctattt
      841 ttgccttgcc ctcttcgtct gagccgatag cgtccatgcc gcaggctctt gcgcatggtc
      901 tttccatttt gtttattgca ttgtatggac agacactctg acatctgccg cactttatac
      961 acttagactg atctattact gacttgccat gcttgatggt tatggcaccc tttggacaga
     1021 cttcctgaca tgggtgcgca agacagccct gacagcagtc ggtaacatga taatgagttt
     1081 caggacagcc gttgcaggca aacttgatta tgtttacaag cggtggatcg taatactttt
     1141 ctgcgatcgc actttcttca atgccgtcag ccacagactt atgctcctcg atcggtctta
     1201 ggggcagacc gatagcaaga cggagccttt cttcaacgat agctctttca aggaagatag
     1261 actcacggta aacagccttg cttcccggta ctatcttata aggaagctct gcgatctttt
     1321 cggcataatc gccctcttca taggcaagtc ttgccacctc tgtaaagact tttcgtctga
     1381 tatctgttac tgaggaatag atacctctca ttttcatatt tttacctcct gcgttgttgt
     1441 tcaaattttt gtcatacact tactattgta ctattttttc ggcttttgtc aagtaatata
     1501 tacttttttt cataaggtat attgaaagtt tcgcatgact tacatatttg tctgaaataa
     1561 tgtgcagtaa tggtacgaaa cggctcaaaa aataaaagta tttctttttt accaatagtt
     1621 tttctgttca tgaaagtatg tggcttttgc acagttttgg gcaggacgat ttatgcaaac
     1681 aaatgataaa tgatttaatt tgacaacatt tctgcaattg ttgcattttt tatgcaacaa
     1741 aaggggcatt tttcgggata agtgagagat gttttttgag agccgttaag atacggtggt
     1801 acatctttcg cagaaattaa aaaaaaggag gaacgatatg gcaggcgaac agaaaagatt
     1861 tgcgagattt ttcttctatc tgagaaatct tcgtgaagcg gctttgcaca ccggtgcaag
     1921 ggcggaggag gctgaggaca gggggcttga gcttctgcac tcggcagaaa cggaggcgga
     1981 gcttaaaaag ctggaggagg ctgacagcga caccctttct tatgtgaagc gagggctgaa
     2041 caggatagct ttcggggcgg tgaatgacgc tgtaaagctt gtttttgacg ctgagaacat
     2101 gacggcaagg gacatcatgg gcatggatct tttcaatgtg agtgagataa agatcagcgg
     2161 aaagaacgtt ttggagatca agttttttga caggcagaaa gccttggaaa agcttgggga
     2221 gcttgcgcct cagcttgacg gcggcggtga gtcgaagcga cttatcgaga gcatttacgg
     2281 cagggacggt gatagcttgt gaaaataaca aggctgtcgg aaaatcagag gtttgttttc
     2341 agatggtgga cggcacggga gcttagtgat tacgacggga taatctgtga cggtgcggtc
     2401 agatcgggca agaccttttg tttgtcggcg tcttttatga catgggcgat gactaatttt
     2461 gacgagtgca tttttgggct atgctcaaaa actatcgtgt cgctgaaaag gaatatcctg
     2521 cctgcgctca gggggtatat gaaagccatg ggcatgacgg ctgtggaagt cgcgtcaaag
     2581 aactatatgg acgttagctt ctgcggcagg aaaaacaggt tttactactt tggcggcagg
     2641 gacgaaggct cgccctctct tatccagggc gtgacccttg cgggggtgct tcttgacgag
     2701 gcggcactta tgcccagaag ctttatagag caggctgtgg caaggtgttc ggtggcgggg
     2761 agcaagctgt ggttcaactg caaccctgac aatccttacc actggttcaa gaaagagtgg
     2821 atagacaagg cggaggaaaa acggcttatc tacagacatt ttgtgctgga ggacaatccg
     2881 acccttgacc gggcggtgat agaacgctat cacaggatat atacggggac gttttacgag
     2941 cgttttgtac ttggcaaatg gacggcggca gagggtcttg tttaccctat gttcgacgag
     3001 gagaaaaacg tctttgaggg ggatatacag tgcgagcggt atgttataag ctgtgattac
     3061 ggcactgtaa atccttcaag cttcgggctt tggggcgaga gtggtggagt atggtacaga
     3121 ctcagggagt attactatga ttctcgacgt gaaggtatgc agaaaactga cgaggagcat
     3181 tacaaggggc ttgaagagct tgccgacggg ctttgcatcg aaaaggtcat ttgcgacccc
     3241 tcggcggctt cgttcataca gtgcatacgc aggcatggaa aattcactgt ccagcctgca
     3301 aagaacgacg ttgtttcggg tatcaggctt gtgtcagact gcataaagga cggcaggata
     3361 aggataaacc gcaggtgtcg tgatacgctt cgtgagataa atctttaccg ctgggacgaa
     3421 aaggcaggaa aggacgcacc tgtgaaagag aacgatcacg ccatggacga tatgagatac
     3481 tttgcggcag aatgtctggg gcgtgaaaag gacgactttt ttgtactgac agttgaacat
     3541 tagtacaaaa aagtgtacag aaagaaagga gagaaaatga gtattttcag aaagaaggtc
     3601 cgtgaatttg tgccgaacac ggcaggctct gacagagagt cggggtttca ttacagtctt
     3661 gcgcctgagg gggtttttca gcaggagctt tatgacgcac tcagggcgaa tgttcctgtt
     3721 atcgacgctt gtttcggcaa gataataagg ctcacagggg gctttaaggt cacggcttgt
     3781 gacgagaggg cgcaagtgga gcttgacagg ttttgcagag aggtttccgt gggggtgtcg
     3841 ggaaagtcca tttacacttt tgcggatatg tatctggatt cactgcttac atatggcaag
     3901 gtgataggca ggatatatgc agattacagg acaggcaagg taaaggggat atatgttggc
     3961 gacccgactt tgtacagagt gcgtgagggc aggaacgctt ttgaaaaacg tattttttac
     4021 atgggaagcg gtgaggagat accggtaaag agtcctgaaa aactgcttta cacggcgctc
     4081 aatcctagtc ctaagcaccc tgacggggtg tccatactcc gtggattgcc tgcgctgagc
     4141 gagatactta tgcgaattta cgagtgcatg gggcagaact tcgacagggt aggaaatgtg
     4201 cgatatgcgg tgacttatca ccctgagggg gagagcgaca gggcgagagc caaggagcgt
     4261 gcagagcaga tagctcagga atggagcaga ggcatgagag cctcacgaaa cggctctgtg
     4321 caggattttg tggctgtggg tgatgtggac ataaaggtca taggcgctga caatcagatg
     4381 atagacacgg agatacctgt cagacagctt atggaacagc ttgtggcgaa gctttcaata
     4441 ccaccgtttt tgcttgggct gaactggtca accactgagc gaatgtctgc acagcaatcg
     4501 gatatcctca caagcgagct tgaatattac cgcagactgc ttgaacctat actcagagag
     4561 atatgcgagg cgtttttaag gcttaacgga tatgcttgtg gggtggagat agagtgggac
     4621 aacataaatc ttcaggacga ggaagcgttg gcgaagtcaa gactttataa tgcgcaggct
     4681 aagacggcgg agcttgaaaa cgaaagggta gagaacgaga gaaaaaagga ggaaatgcaa
     4741 tgagcgaaac agtatcaggc gaggagcttg aaaagataaa cggctatgcg agagagccgc
     4801 tcacggagga caaggtcttt gttttcaggg tggcgctttg tgacaatgat attgacagag
     4861 atggtgaaaa gttttcatca ggcgctttgg agaagcttgc ggagcttttt aagggcagaa
     4921 cgggtatttt cgaccatgac cctaaaagct caaagcagac tgccagaata ttcgacactt
     4981 gggtggaaac tctgcctgag aaaactacga cagacggaga ggtctaccgc aggcttatgg
     5041 caaaggctta catggtgcga actggttcta acggcgacct tataagcgag atccagggcg
     5101 gaataaagaa agaggtatcc gttagctgca ccatgggaaa gaagctttgc tctgtatgcg
     5161 gagcggatat gtacaagggc ggctgtgacc atgaaaaggg cggtgaatac ggcggtaagc
     5221 tgtgttatca cattcttgac gagccgcttg acgcttacga atggtcgttc gtggcagtgc
     5281 ctgcacaggt gaacgcaggg gtaacgaagc gttttgcttt aagggagaag caggaaagca
     5341 ccgacaagag ctatgagctt gcccttgcga gggaagcctt tgaaaaggac gttttaaggc
     5401 tcagctattt ttgcaagcct tttatgagtg caaagcgtgt gaaagaactt gcggagctta
     5461 tgacagttac tgagcttatc gacttccgtg gcaggcttga aaagcaggcg gctgagaatg
     5521 aggaggtcta caaagccgaa agggagagct ttatcacggg ggattacaag atgtaaggga
     5581 cagatgtgaa agggggcgat gacaatgtgg agataggggc ttaaatgcca gaatgtgata
     5641 tcatcactgt aggggcgaac agtgttcacc cgaaaagaca aatggttttc gatgatacga
     5701 aaatcagtac aaaaaatcgg acaaatttaa ttatcgaaaa ggagaaaata atatgtacaa
     5761 caatatcaaa cttgaaaagg gactttattc tatcacaggc aagacattca cacaggcgct
     5821 ggctgagctt gatcctgacg caaactatga gaacaccgag cttaaaggtc ttgacgcttt
     5881 tgaaagacag ctcaagcgtt ttgacatcaa ggtaaagggt gcaaattctg acagggtgga
     5941 gaaattcttc atttccactg agtcggcagt gctttttcct gagtacataa gacgcactgt
     6001 caagcagggc atggacgagg cttctatcat gggcaaggta gcggcggctg tttcatacac
     6061 tgacggagtg gatttcagag gtctgaacgt tacaaagtca ggttcaactg acgttgtggc
     6121 agagggtgga aatgtgccta tgacaactgt cagacttagc acctccagta agactcttac
     6181 aaagtttgca agacgtctta actgttcttt tgagtctgta aggaagcaga agcttgaggc
     6241 tttcggcgtg gtgctgagaa accttggagc gaccataagc agagatgtga acagctttgc
     6301 tatgactgag ataacaagag gtattacagc tcagactctt attggcagtg agcttactta
     6361 cgctgatctg gcggcttttt gggcttccat gggcgagttt aacatgacga ctatgatctg
     6421 tactcctgat gttatggcta agatccttgc tatggacgag atgaaatact gcattggcga
     6481 ctatatggca ggcggcacag ttcagacgcc atatggtgta acgcttgtaa aatgccctca
     6541 gctcactggc ggtattgcgg tgggcatcga ccagagcagt gctgttgaaa tggtgcttgg
     6601 aggagatgtt atcgtggact acgataagct tattactaat cagtgcaacg agatcgtttg
     6661 ctcagtgctt gcaggtttct ctgtacttac gagcggtgct gtaaagacgc tgaaaactac
     6721 aaagtaagct gagaggataa tgaaaaaagg gacggtgggc gggtatattt tttgaaagga
     6781 gcaggaaaat ggcaataagc aaagacaatg taaagagcaa tttcatggag ctaagcggac
     6841 ttgacagcac gtcggctgag gtgtatgcag ggcttataac ggtgtgtgct gacgaaatgg
     6901 aaaaggctgt tgaccaggag cgaatggtcg ctgagggtgg gacggctata tgcgaatttg
     6961 cggcggcagc ggaggtcttt tacagattta tttgtctgaa agccgctgaa tacaaggtca
     7021 tgtttacggc acagggcaag gcggtggagg cttttgacga ggagaacagg ataaaggcgg
     7081 caagagagct tcgtgacagc gcagtttcaa gggcggaaag atttttctca aaggacggct
     7141 ttgtattcaa tgccgttatc gcatattagg aaggaggacg agccatgaca gtttggacag
     7201 aaatggcggc agagataaaa aagatacttg acggagcagg ctttactgca atgggtgaat
     7261 acacatcggc tgacagtcta ttgcacagtg gggaagcaat tggcttctat ggacttaaag
     7321 gctatgatgt ggtcgatgaa gtcattgggc agggcggcaa aaagctttac actgagtata
     7381 tatgtcatat cgacctgcgg ctcatgggca aggcaggaaa atacgatgac atggaggagc
     7441 ttcagggaaa gtgtgaaagc gttatggcgg agcttgcaaa aagcgaggtt ctgaatgtta
     7501 agagcgcaaa gcttggaggc gcagttcaga cattgcccct tcacaggctc gaaaggcagg
     7561 cagagcttag tatcggcatt tgcagtgagg aggatataag tgagtaaaaa gagaaagata
     7621 aagcttgaca ttggcacacg gtcattttac atggacggag ttaagcgaag cggaggttac
     7681 attcaggagg aaacaaaggc tgtgaatggt attttgcgaa atatctcagg caaggcggcg
     7741 gaaatatttc agttcagcgg caggatcgct ccgtcggaca aggcttacgc ttgggctgct
     7801 ctaagcgaga acggaggaaa ggcgatcaat ttcacggcag acggggtctt atacagcgga
     7861 tacatactct cagagataga aatggatttt cagcctgaca ggatgatcgg aagccttatg
     7921 ataaagctta ggggggcgga ataatggcga agatatacat caggtttgaa cttattggca
     7981 gtggtaattt ggactataga aacattgttt cagcaaggct tgtgaaagac cgctatacgc
     8041 catacagtga acttaaagcg tctgtcgttc tgaataacag cataaatctt aacaatatca
     8101 ggcgtgtggc actttatata ggcagcaggc gtatgcacta cggtcctgcg gattacatca
     8161 gaacaagggt gagaggcgga aaaactgttc ttgaacttat aagccgtggg ataacactta
     8221 tgctggggca gaacgagcct gaacccgggg tatggtctaa ggtgagtttg acggatatta
     8281 tgaagagcta cagtccctca agcgagataa cctacgagag tggaactgac actgtgaact
     8341 atgtgaacat caaagagaag tctacactgt gggaggctat tggtgtatat gcggtcaagg
     8401 cttacggcag gcgtgcatat atcagagggg acaggcaggt cagtgtgtcg ctgagtatcc
     8461 tttcggtgaa tatcggtact cagcgtatta ttgaatacgg caaacagctt gatacgagat
     8521 cgatgctgtc gaagatatat atgaaaaacg tggacgggga gtatggatat agctattcaa
     8581 gcggtaaaac tataccacat ggtatcacaa gggagaagta ttatggtctt gataagcagt
     8641 ggctcaataa tgtagttgtc gggcttaaag ataggataga gtttgcagag agggagtatt
     8701 tggtggagca tatcacttat gaggggtatt gcggtgagga tatcacggat aaaataattt
     8761 gtaatggata tggcgtaaat ggaaaaagag tgagcagaat ggagataacg gtcaatagca
     8821 aaggtatgca aacgaagctt ttatgtgaat aagaaagaga cgtaaggaat gaatagagca
     8881 aaaaaaacag cgtacagaaa aactgtacgc tgtttgaagc ttcgtattaa ccctttacgc
     8941 ttcccagagc aacaccacga atgatgaact ttgaaagaca aaggtaaaca attactacag
     9001 ggaggatagc aagtacgatg tacaagctga acattcccac gtccttctcc tgtggtcctg
     9061 cataacgcat tgcgttgagt accagaggga tcgtgtactt actctttgat gtaagaataa
     9121 gtcttggcat aaagtagttg ttccagtttg ttacgaatgt aaagatgatc tgaactgcga
     9181 atgcaggctt gaggatagga gtaacgatct tgttgaaggt cttgaactct ccgcagccgt
     9241 cgatacgggc ggcttctacg atctcaagcg gaagtgcaga cgccatatac tgcttcatga
     9301 agtagtatgt agctggtgct gcgacagcag gaatgatcag cggaatataa gtatctgtcc
     9361 agccaagttt gttcatgaac tggtagaaac cgatagctga agcctgtgtt ggaaccatca
     9421 ttacgatgag aatgaacata tctgcgaact ttctcagctt gaactggtaa acgtgtgttg
     9481 catatgctgt cagtgctgag aagtatacgc tgagcaatga agcacaagct gatatgaaca
     9541 gtgagttagc cagtgaacgc catacgcttc cgtatgctac ggctgttgaa ccgctgaaaa
     9601 ggttcttgaa gtttgttgcg aggaagtggt caggatacca tctgatacct gcctgtgatc
     9661 tgcctcgtgt tgcgttgatt atcaaaagat agaatgggaa aagtgaaaca acgaccaaaa
     9721 gaacaaggac aacataagct attatcctat gtatgataag accagtacta tttgtctttt
     9781 caccaactat ttcagtactt cttggcattt cagcttaccc ccttttcttc tttttcttct
     9841 ttccgtcatg gtcagcagac gcaaacataa gtgcaagtcc gataacagcg gaaacgataa
     9901 agattattac gcagagtgca gacgccttac cgatgtcgcc ctgcttattt gcctggatta
     9961 tcattacgac ggtcttaagc tttccgttag ggctacccat atcctttgtg aggagggcag
    10021 gtacgtcgta catctgaagt ccaccgatga gtgatgttac aagaacatat gacatgatag
    10081 gcttgatgag cggaattgtg atcttccaga atgtctggct ggaagttgca ccgtcgatct
    10141 gtgctgactc atagagtgat gtatcaacac ccatgatagc agccataaga agaattgttg
    10201 tattaccata ccaaagtatg aagttgatga gtccaactac acctcttgat ccccaaacgc
    10261 tttcaaggag tggtaccttg ataagatcat agatagcgcc gcctctgctg aacaagctgt
    10321 agaacagaaa tgcaaaagca gatgccatga ttacgtttgg aagatagata acagccttga
    10381 agaatccctg agcctttatc ttaagacgaa ggtcagtgaa ccatactgca aggataagag
    10441 agataacgat ctgtgggata aatccaacga tccacatgat gatcgtattc cagaatgact
    10501 ggagaaggtc acttcctgat gtgaatatag ttgaatagtt atcgaaaccg cagaatgtcg
    10561 gtgttacttc gacagcctgt cttgtccaac caccgccgat agagccctga tccttaacat
    10621 agtctgtaaa gctgtaatag aaagtctgga acagaggata tagtgagaag atgatgtaaa
    10681 ctacaaagaa tgggataagg aaaacatatc cccacttggc atagcttatg ctttttctct
    10741 tcatataagt atacctattc ctttcgtgag attaataaat aatagggggg gataacctcc
    10801 accccctata tttaattaga tactgttttt tatacgatta gaaatcgcaa gtaaggttac
    10861 catagaggtc tgtgatgttc ttcttgaagt tctcaagagc agcctcatat gaatcaactg
    10921 tgccgaggaa gtagtccttc atagcaccct ggaatttctc gttgcagccc tggtcataag
    10981 cagaaagctt gttctcgagg gagatcttgt cagcagcctc tgtaagaagt gcaaggtgat
    11041 cctgtccgcc gaggaatgcg tttgtataac cgccgtcgat aagttccttg atagcagcct
    11101 tgttgtttgt gtagtcctgc tcgccctcag tgatagcctt agctacgtcc ttgttgcaag
    11161 tcataacctt catgatatca gcaacgatat cctggttatc ggagtcgata gcgccgcaga
    11221 tccatgtacc gccccagtag taagcctgtg ggcctgagca agccttccag agaccataac
    11281 cgccgttgcc ttccttagcg tttgaaccat cagccttgat ctcttcgtca actgtgtttg
    11341 gaagaagtgt gaaaggaatg ccccatgttg agaagaagta gccgaatacg ccgccgtcga
    11401 tcttctggcc agcagcccaa gcatcgtccc aaagggaagt cttgttgttg tagcccttgt
    11461 ctgtgtattc cttagtctgg tcagcccaag ccttgatctg tgggtcaact gtgatcttgt
    11521 tgtctgttac ccatgggcta gacatattgt ttgagaagca tctgtatgta tcatcgaaac
    11581 cagataccat cttgtagccc ttgtcagcca tcttctgagc tgttgctgtg aatgtatccc
    11641 aatccttaac agcttcctga accttctctg gatcgcttgt gccgagaacg tcctcagcga
    11701 tctttgtgtt gtagaggaac agacctggag ttgcctgcca tgatacaccc ttcagctcgc
    11761 ctgtcttggt atctgttgca ccgtcctttg tgtactgata catctgtgac atatcgtcgt
    11821 ctgtgatgcc gaggtcctga atagaaagtg caacgtcgcc gttagcatac ttcagagcgt
    11881 agtcagcttc cataaggaac atatctacct tctctgtgct gtcctgctga cccttaagag
    11941 cctcgtccag ctttgtctgg taagcgttac cttcgttttc aacctgtacc cagttcagct
    12001 taacgccgtt gatcgttgcg atctccttag aaccatcagc aagcttgttg tatgtaagct
    12061 catttgatgt gccctgtgga tagtacttat caagtcttga cttgaactct gtgttccagc
    12121 agtaaattgt aagagtcttc tttgaatcct ctgatgaatc tacaccgtgg agttctgctg
    12181 tgtttactct gtcagagctg ttgttgatac ctgtgccgtc gccagccttt gatgtgctgc
    12241 cgcccttaac atcagttgct gaagatgaat ctgagctgcc gcaagctgtg aacattgaag
    12301 ctgccagaca gaggctcaat gaacctgcaa gtactttctt aagatttgcc ataataattt
    12361 tcctccttaa aatcgcatta gtttatttga tttgctccgc agcatttctg cggaaaatta
    12421 atgccttgaa tcagttgatg tcgcttaccg agccgccttc cagcagcttt ccgtcgactg
    12481 ttatcacctc tttaaaggtg gtctgtgggt tttctatcag tgataccagc tgttttgcgg
    12541 ctgttacacc gataaggtct gtgtcctgaa gataagtcgt aagctttgga gagagaagct
    12601 gtgaataagc tattccgtcg tagcccacta ttgagatatc ctccggtact gaaagaccca
    12661 tttccttgac tgcattgaaa gctcccattg ctgaataatc atcgggcatg aaaacgcagg
    12721 tcggtttgtt ctctctgcta agaagttctt ttgtcagctt gtaagtcaga tcagggttgt
    12781 gataatcacc gttaagtatc caatcgggat caactgaaat tcctcttgtc atacaagctt
    12841 tatagaagcc tgccagacgt ctttcggcta ctgccgatct gttgccctgg ataaaagcga
    12901 tcttcctatg ccccatcttc gtcacatagt tgataagctc ttccatacct ttttcattat
    12961 tggacatgat cgctgttctg caatcgaaga tgtggtcgat cgtgaccact ggtatatctc
    13021 cgtttatcac ctcgtacacg tcctgcgacg taaaatccgt acaggcaatg ataacaccgt
    13081 caacattgcg gtatttgcaa tgttcgtatg tagacatttt ctgcttgccc acatctctat
    13141 ttataaaggt tatatcatat cctgctgctt cagccgtagt cttaaagctg tcaagcacct
    13201 ttgcgaaaaa ctcatgggta agaccgcttc ctgcttcgtc gagatacatg accccgagat
    13261 tataggtcct gttggtcttt aaagctcttg cctgagaatt tgggaagtag cccatttctt
    13321 tggcggcttt cataagtctt tctcttgttg cctcgcttac gtccttatgg ccgttgaggg
    13381 ctttgcttac ggttgcgacg gatactccgc agcgaacgga aatgtctttt aaggaaacca
    13441 atggtatttc cgcctttccg tagattttaa acgtttacgc ttgatgtttt ccatgaaaac
    13501 ttatttcaaa cgtttccgtt aataaaaata tacaatattc tcggcataat ttcaagtctt
    13561 ttttcacat
//
LOCUS       DAWEMR010000063        13264 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_1961_length_13264_cov_13.074268,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000063 DAWEMR010000000
VERSION     DAWEMR010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13264)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13264)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13264
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1961_length_13264_cov_13.074268"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3761 a         2869 c         3342 g         3292 t
ORIGIN      
        1 tttttctttt gctttgatat acttattgcc gccgttactg cccataaaag cagaagtatc
       61 accactatcc agtcggctgt attcataata acgctcctat ctgatatacc gcacagccgc
      121 aaagccatgc caccatgcac tgcattatca caacgcctac tgtctgtatc cttgaatcaa
      181 gctctctctt tatggtcgcc accgccgcaa cacatggggt gtaaagcagg gtgaatacga
      241 gaaagctcag tgcggaaagg gtggagaatg ctcccgaaag agcagtgcca agatttgcag
      301 tgcttgtgtt catcagtacc gccattgtgc ttactaccgc ctcttttgca gtgaagcctg
      361 atataagcga ggtggtcact ctccaatctg caaagcccaa aggcttgaat attggcgata
      421 tagcctgacc gataagagct agcaggctgt cggaggagtc ttttacaacg ttgagctttg
      481 cgtcaaaggt ctccaaaaac catattatga tagtcgcaaa gaatataact gtgaaagctc
      541 tttgtataaa gtcctttgcc ttgtcccaaa gaagaagtcc tacgcttttt gccgaaggaa
      601 gacgatagtt tggaagctcc attacaaacg gcacaggctt gccacggaaa gctgtatttt
      661 tcagtatcag tgcaactatt atcccaagca ctatgcccgt tatgtataag cctatcatca
      721 caagcgcctg atattttttg aaaaatgctg cacagaaaac tgcgtaaata ggtatctttg
      781 ccgaacagct catgtaaggg gtaagaagta tggtcatttt tctgtcacgg tctgatacaa
      841 gcgttctcgt cgccattatg gcaggcactg aacagccgaa gcctatgagc ataggcacaa
      901 agcttcgtcc cgaaagaccg atctttctaa gaagcttgtc cattacgaat gccactcgtg
      961 ccatgtagcc cgtgtcctcc agtatggaaa ggaaaaagaa cagcgtcact attataggca
     1021 gaaagcttag aacgcttcca acgcctgcaa aaactccgtc tattataagg ctgtgtacaa
     1081 cagggtttat gccgtaggct gtaagccctt tgtctgctag gtcggtgagc ttgtctatac
     1141 caagggaaag ccaatcggaa agggtcgagc cgataacatt gaatgtgagg aaaaatacaa
     1201 gaaacattat cccgaaaaat acaggcagtg cagtgtattt tcccgttaaa atgcggtcta
     1261 tttttacgct ccgcttgtgt tctcggctct cgtggcattt cacaacgctt atggcaacga
     1321 ctttttcgat aaagtcgtat cgcatatctg caagcgccgc atttctgtca agctttgatt
     1381 cgtcctccat ttctactatg cagtgttcga gaagctccag ctcgttttga ttaagctcaa
     1441 gctgatcctc gatattcttg tcaccctcaa taagctttga agcacaaaat ctcggcggta
     1501 tgccaactcg attagcgtga tccatgataa gatgcacaac tgcgtgtata catctgtgca
     1561 cagcggaatc atctgagcaa aagtctgtga caacaggctt tgtcttgttt cgggcagttt
     1621 ctacagcctt gtcgataagg tctgagatac cctcgccctt tgccgcactt actggaataa
     1681 caggtatgcc aagtgcgtcg ctcatcattt ttacgtctat cgttccaccg ttggctctta
     1741 cctcgtccat catgtttaag gcaagcacca ttggcacacg cagttcaaga agctgtaaag
     1801 tgagatacag atttcgctct atattggtgg cgtcaacaat gtttataatg ccgtcaggct
     1861 tttcatttat tatgtagtca cgggtgacga tctcttcctg cgtgtaaggg cgcagtgagt
     1921 atattcccgg cagatccacc acagtgcagt ttttctgccc ctttatgtca ccgaccttct
     1981 gctcgactgt tacaccggga aagtttccaa cgtgctgatt agaccctgta agggcgttaa
     2041 aaagcgtggt ctttccacag ttctgatttc ctgcaagggc aaatctcata tatggtcatt
     2101 ctcctctgtt ttttctatct ctatgttttt tgcgtcctct atcctgagcg tcagctcata
     2161 gcctctcagt actatatcaa gagggtcgcc cattggcgca cgctttagca ttttcacctt
     2221 tgttttgggt ataagtccca tgtcgagaaa acgcagccta agcgcaccct cgccgcctac
     2281 cgcttttatc acggcagact cacccgtttt aagcttatca agagtcattg tcttttcctc
     2341 catagctata ttttattctt atgttttatc taaaagaact ctacaaaata agtttaccat
     2401 ggctaacttt acttgtcaat agtatatgtc atatatttta gtcagatgtt atctaaaaat
     2461 tttattcaaa tagataactt ccaattgaca acgccaacaa atcgtgctat cataaagtaa
     2521 gataaagtac acttttacat tacgggggtg ttttatggat acccgtgtgg caatacttgc
     2581 aataatcgca cacgataacg gctctgcgca ggagataaac gcaattcttc atgagcatgc
     2641 cgagtatata ataggacgaa tgggtctgcc ataccgagag cgtggcatga acataataag
     2701 cgtggcagtt gacgctccgc aggacgttat aaattctctt gcaggcaaga taggcaggct
     2761 tgagggagtg accgccaaga ccctttattc gccacagtaa aaatctaata aatatttgtg
     2821 accctgacgc cgaaaacgag ttttctcctt taaggcgaaa gaatacacat caagcagaaa
     2881 aagtaaaaag cagatagaaa gccctatctc tactcatgta ggggcgaaca gcgttcgccc
     2941 gtggattagg ttttttatga gcaagcatag ccttttttgt tatcaaagct gtatcctcag
     3001 gggtatggct tatttttttt gagaggagct tttgcaatgg gacttaatga cgcaccaagc
     3061 ggagaacgta tacatatagg atttttcggc agacgaaacg caggaaaatc aagcgttgtg
     3121 aatgcagtaa caaatcagga tcttgcagtg gtatccgata cgaaaggcac taccaccgac
     3181 cctgttacaa aggcaatgga gcttttgccc cttggccctg ttgtgataat agataccccc
     3241 ggctttgacg acgagggcga gcttggagag cttcgtgtga agaaaactaa gcagatacta
     3301 aacagaacag actgcgcagt tcttgttacc gattgcacct tgccccttgg cgactgcgaa
     3361 aaacagctta tctcaatatt caaagagaaa aacatacctt ttatcatagc caaaaacaaa
     3421 gccgacctgc tcactgatat cccaaaagag gatggcgcag tgtacgtcag cgcaaaagag
     3481 aaaacaggca tagaggagct taaaaacgct gttgcaaagc tcactgaaac agacaccatg
     3541 acaatgaagc ttgtgtgcga ccttataaag cctgacgata tggtggtgct tgtcacccct
     3601 atcgacagcg ccgcaccaaa gggcaggctt atcctgccac aacagcaggc cataagagat
     3661 gttcttgaag caaacgcctg tgcagtggtg gtaagagaaa ctgagctttc aggagtgctt
     3721 ggcaggatag agaagcctgc acttgttata acggatagtc aggctttcgc aagagtttca
     3781 aaggatactc ccgaggatat ccctctcacc tcgttttcta tccttatggc tagatataag
     3841 ggctttcttg acgccgcagt aaagggcgtg aaagctatcg acgatcttaa agacggtgac
     3901 aaggtgctta tttcagaggg ctgtacacat cacagacagt gcggtgatat aggctctgtg
     3961 aagcttccga actggctgaa agaatacaca ggcaagaatc ttgagatagt gctgtcatca
     4021 ggtcacggct ttccagagga gctttcagac tttgcgcttt gcatacactg tggcggctgt
     4081 atgctcggct caaaagagct tacctacaga ataaaatgtg cttgtgacgc aggcgtgccg
     4141 ttcacaaatt acggcattgc aattgcctac atgaaaggta ttctcaaacg tagcatagaa
     4201 gtgtttccac atcttgtaaa ggagcttgaa gatcacaatg gcggtcagag aacttattga
     4261 taaactaaac tgcgagaaaa agctttccca cgatgaatgg gtgaagctta taaaagaata
     4321 tgacgagagc gacagaacct atgccgcaga gcttgcaagg tgcatttcgc aggaaatatt
     4381 cggcaaggat atctacataa ggggcatagt ggagttttca aacatctgca aaaacgattg
     4441 cctttactgt ggaataaggc gttcaaacgg caacgtgagc agatacaggc tcaccgctga
     4501 ggagatcttg cagtgctgtg acgagggcta cggctatggt ttccgtacct ttgtgcttca
     4561 aagcggtgag gacgctttct atgacaggga aatgctctgc ggtatcgtca gagagataaa
     4621 aaagcgtcac cctgactgtg cggtgacatt atccataggt gagaaaagcc gtgaggacta
     4681 tgaagccttt ttcaatgcag gtgtagaccg gtatcttctc aggcacgaaa ctgccgacga
     4741 ggagcattac aaaaagctcc acccagccga aatgtcttgg aaaaaccgaa tggagtgtct
     4801 ttcagatctt aaagagatag gctatcaaac aggctgtgga atgatgatag gctcgccgta
     4861 tcagacggtg gagtgccttg ctgaggatat ggagtttatg tgcgatttca agcccgaaat
     4921 gataggcatt ggaccttttc tcccacataa ggacacgcct ttcagaaact gtccacaagg
     4981 ctcatatgag cttactctct ttctgctgag catttgcagg ataatgctcc ccgacgttct
     5041 tttgcctgcc accacagccc ttggtactat aaacccaaaa gggagagaac aaggagtgct
     5101 gtcaggtgcg aacgttataa tgccgaatct gtcccctgtg gcagtgagaa aaaaatatat
     5161 gctctatgac aacaagatat gcacaggtga cgagtcagca cagtgcaggg cttgccttga
     5221 aagacgaatg gagtccatag gctataaaat aaagatatca agaggagatc acagataatg
     5281 ataaagcttg ctttttacgg aaaaggcgga ataggaaagt caacggcggc atcaaacgtc
     5341 agtgctgcac ttgcacaaaa agggctgaaa gtcatgcaga taggctgtga cccaaaggct
     5401 gactccacca tatttctcca tgggggagag agaataccga ccgttcttga ccttgtgcga
     5461 aaaggcgaaa gctttgacct tgaagatatg gtaagggtag gctataacgg tgttgtgtgc
     5521 gttgaagcag gcggtccggt gccggggctt ggctgtgcag gcagaggtat aatcgccgct
     5581 ttggaggagc ttgaaaaaaa gggcgcatat gaaaaatatc agcctgacgt tgttatctat
     5641 gacgttctcg gagacgtggt gtgcggaggt ttttcaatgc caatgcgaaa gggctatgcc
     5701 gataaggtgt ttatcatcac gtcaggcgag agtatgtctg tatatgccgc cgcaaacatc
     5761 gcaatggcgg tggaaaattt caaaagccgt ggatatgctt ctcttggcgg ttttatcctc
     5821 aataaacgca acgtaaaaaa cgaggaggaa aaagcggcag agcttgcggc tgattttcac
     5881 agctctgtga tagccgagat agaccgaagc gaccttgtga gcgaagctga ggatatgcgc
     5941 aaaactgtca tggaagcttt tcctgacagc actgcggcag agcagtataa agcattggcg
     6001 gataagatag ccgagctttg cggtatggga gaagaaaaat gacaaaagag agcggcataa
     6061 atataacgaa ggcggctttt cctgcgcctt ttgaaagcgg tcttgaatat aatcccccca
     6121 caaggggcgt gtggaatatc gtccatacag gtatgcttat ccctgagtca cggcagatat
     6181 ttgtctgcgc acagggctgt ttaaggggag ttatcctaac tgcggcagag atgaatgcca
     6241 tggacagaat gtcatgggtg actgtttctg agcaggatct gtatgacggc acaatggagc
     6301 aggacgttat agacggagtg agggatataa taaacaggct tgaggaaaag ccaaaatgcg
     6361 ttttgatttt tctaagctgt gttcaccttt ttgcaggctg tgacttcaaa atgataatag
     6421 acgagctttc gacattgttt ccacaggtgc attttataga ttgctacatg acgcccacca
     6481 tgagaaagtc tatctccccc gacagcctta tgagaaaaca gctttacgag ccacttgaaa
     6541 agtgcgaaaa gaagcctaaa acggcggcaa taataggctg tgacagagcc acggacgaag
     6601 cctccgagct tgtaaaaata ataaactcag caggctttga gcttcttgac ataacaaagt
     6661 gcaagaccta tgaggagtat ctctccatgg cggaaagctc tttgaatatc acctatctcc
     6721 ccacggcaaa gccgtcagga gaggaacttg aaaagcgact tggcacaaag catttgtatc
     6781 tgcccttgtg ctatggctat gatgagatag agaaaaatta cagcagactt tgcacccagc
     6841 ttggtgtgaa aacgcctgac ttttctgctg acagagaagc cgccgacaag gctcttgcaa
     6901 atgcccttga ggtagtaggg gatttgccta tagccgtgga ttataccgct ctgccaaggc
     6961 cccttggctt ttgcagactt cttgcagaga aaggcttcaa tgtgaagcgt tgttatgccg
     7021 acagcttttc agaggaggaa agggcagact ttgagtggct gaaagcccat tgccacgacc
     7081 ttgaaattct ccctactgtg aatgtgaaaa tggagttttc acagtgcgaa gaaaaatatc
     7141 ttgcagtagg tcagaaagcg gcttttttct gccagacaga taactttgtt gatatagtcg
     7201 cagggggcgg gggatacggc ttcgagggca taaaaaggct tgcagagctt atggctgagg
     7261 gagcagtaaa caaaaaggac agacgtaagg tgatacaaca caagggctgg gggtgtgaaa
     7321 gctgtctatg aatgcaaaag tgatatccat atatgcggcg gatacttcgg gagtatgctc
     7381 ggcgctttat gagcttggtg gaatgaccgt cgtccatgac gcttcgggct gtaactccac
     7441 ctacgccacc catgacgagc cacgctggta tgaccgcaaa agcatgatat atatttctgc
     7501 actaacagaa gcggacgccg ttatgggcaa tgacggcaag ttcattcgtg atgtggcaaa
     7561 agcggcaggt gagctttcac ccaaattcat agccatttgc ggttcgccaa tgcctgcaat
     7621 gacaggcttt gactatagtg cggcggctga ggagatagag cgtcaaacgg gtctgaccac
     7681 atttttcgtt gataccaacg gcacacattc ttatctccat ggagcagagg gggctttcct
     7741 taatattgca aagcttttct gccgtgaggg aaaagaaaag caggcaaatt cagtgaatat
     7801 tattggtgca acgcctcttg acttttcggt gaatacaagc gtaagctcca taaaaaagtg
     7861 gcttttggat aacggctttt cagtgcaaag ctgttttgct atggacagca gccttgatga
     7921 gatctctgcc gcaccacagg cggctgtaag ccttgttata tcctcagacg ggatagctgc
     7981 ggcaaagtat ctttttgata cctatggagt gccatatgtt gtgggcgtgc ctgtgggaaa
     8041 aagcttttcc aaaaagcttt ccgctgacct gaaaagagca gtgtcagagg gcgtttgtat
     8101 aaattcctgc ggtgaaaaag ctgtagagaa tgctcatatg atagttgcag gggaaagcgt
     8161 ttttgcttct tcccttgggg ctgaacttgg cgcaaaaact gtggcgacag ttggcataag
     8221 aaacagcgag gtgctgtcag ggacggacat tttctgcgag gaagaagcag agcttgaaaa
     8281 gcttttttca cagcataaga ccgtcatagc agacccgctt ttcagaccta tatgcaaagg
     8341 ggcaagattt atttcactgc cacacgttgc tttttcggga agatgttttc ttaaagatat
     8401 ccccgatctg atagataaag atgtaagcaa aatacttaac ctataaaaag gaggaacgtg
     8461 ctttcgcacg gatactttta tgataaaaat agccgtttac ggaaagggcg gaataggaaa
     8521 atccacagtt acgggaaacc ttgccgccgc atttgcaagt cttgggaaaa gagttataca
     8581 aataggcttt gacccaaagg ctgactccac cataaatctt cttgggggcg agcctgttat
     8641 gcctgtgatg aattatctca gagagcatga cgatgagcct gagagcatag aggagatatc
     8701 aaaagagggc tatggcggag tgctttgtat agaaacaggc ggtcctaccc ccggacttgg
     8761 ctgtgcaggc agaggtataa tcaccacgtt cagcctgctt gaagacttaa agctttttga
     8821 aaaatataag cctgacgttg tgctttatga cgttctcgga gacgttgtgt gcggaggttt
     8881 cgccgcacct atcagagagg gctacgcttc aaaggtgctt atcgtcacct caggcgagaa
     8941 aatggcattg tatgccgcaa ataatataaa cagtgcggtg aaaaactttg ccgacagaag
     9001 ctatgcaaaa gtccgtggta tcgtcatgaa caggcgaaac gttgagggcg aggaagaaaa
     9061 ggtcagagcc tttgcagaaa gcgcaggact tgatatcgtt gcggatatac caagaagcga
     9121 cgatatcatc agatgtgagg ataagggcat gaccgtcata gagggcgagt ctgattgtca
     9181 ggcggcaaaa tgctttttga agcttgccca gacccttatt caggaggaca acgatgacta
     9241 aagcgtttta cataacggcg gcggagcttg cccaaaaggg aaaagataat atccctgacg
     9301 agcttctttc ggcaaagcac cttatctaca gctcccctgc aacattggcg ttcaattcac
     9361 cgggagcgca gggctttggt gtaaaaagag caggacttgc agtgccaaat tcggttatgc
     9421 ttcttgtggc tcctgcctgc tgtggacgaa acaccactat gctcagtgcg gaaagcggct
     9481 acggcgacag atttttctac ctgcttcttg acgataccga tatcgtcaca ggcaggcacc
     9541 ttacaaagat accgaaagct gtggaggagg tatgtcaaag ccttgatcac actccctcgg
     9601 cggtgatgat atgtataacc tgcgttgacg ctctcctcgg cactgatatg gacagagtgt
     9661 gcagaaaatc cagcgaccga gcaggcgtgc ctgttgtgcc atgctatatg tatgccctca
     9721 caagggaaaa gcgtctgccc cctatggctt cagtgcgaaa atcggtgtat tcacttttga
     9781 aacctcgcaa gagaaagtct gacgaggtga atatcctcgg ttatttctca cccttgcagg
     9841 aggattgcga gctttacagt ctgctcaaag gcgcaggagt aaggaagata agagagatat
     9901 caaggtgtga aagctttgag gagtatgagc agatgtcgca ggcaaatttt tctcttgtac
     9961 tcaatgcgga agcaagatac gccgcccagc aaatggagca ggagcttaac atacctttta
    10021 tagagcttgc gagagttttt caggccgaca agatatctac gcagtaccgt ctgctgggtc
    10081 aggcgatagg agcggagctt tgcgataaag agtattatga gcagaccatg gctcttatag
    10141 atgatttcag agaaaagcat aaagggctgt gctttgcagt gggcgagttt tcaaactgcg
    10201 accctataga aatgtctttg tcacttataa gatacggctt caaggtggcg gagatattcg
    10261 gcactgtggg ggagcgaaat ttcggctttg tgaaaaagat agcccagctt tcccctgaaa
    10321 caaaaatata ttcaaatctc tcaccaacaa tgctctttta cgagcctgac aaacgggtga
    10381 acgtttatat cggcaaggac tgtgcctatt accaccctga catagcagga gcagacttca
    10441 atgacgagat acagcctttc ggctatgcag gagtgaaaaa gctgttttgc gaccttgaaa
    10501 aagcgcttga caggaaagga gcggaggata aatgaaaaat cttttgaagc ttttgtcacc
    10561 ctttgcccct gaccaatcag gtgcgtctgc agtgctctat gagcttggag gactaatggt
    10621 aatatgcgac gcaggcggct gtgcaggcaa cgtctgtggc tttgacgagc caaggtggtt
    10681 tacaaaaaag agcgcagtct ttagtgcggg gcttcgtgat atggacgcaa ttttaggacg
    10741 tgacgacagg cttatcgaaa agctctccaa agcctgtgag cagataagac cagcctttac
    10801 cgccataata ggcacacctg tgcctgctgt catcgcaaca gatatgagag cgttgaaacg
    10861 aatggcagag aagaaaacag gacttccttg cataacagcc gagtgtacag gcacgaacta
    10921 ttatgacagc ggctcagagc cggtgtggat agagcttttc aagacctttg ccactgagag
    10981 caagcctgtt cagaaaggca ggcttggcat tataggtgca acgcctttgg agcttagtat
    11041 cataagcccc gagaggatag tggcggaata taaggcaaag ggttgggaga atgttatatg
    11101 ctacggcatg ggaagcaccc ttgacgatgt gaaaaatgcg gctgcggcag agatgaacat
    11161 agtctgttca gcagcgacaa taaaggcggc ggagtatctc aaagaaaagt tcggcacgcc
    11221 ttatgagata gaatatccac aaagctgtct gcctgaggag atagtgcaaa aagctgataa
    11281 ctttacggat aaaaaagtcc ttgtgataca taaccaaaca gcggctgacg ctgtgagaaa
    11341 gcttatcaca gaaaaaagcg gcaaatgcaa ttgcacatcg gcaacatttt ttatgcagaa
    11401 aaaagagctt tcacagcaga gtgatttttt ccttgcagac gaaagcgacc tttgcgacaa
    11461 tgttggtgat aactatgacg ttgttatcgc agacaagacc ctcagacgtg ttatgaagaa
    11521 tttcagcggc gagtttatag actttccgca tttcggactt tcaggcagac ttttgcaggg
    11581 agagagtgaa aagatatgac agaagaaatt atagagcgtc ttgaagaata tctcggctca
    11641 gaggagtttg aatatgaagc cgaaaactgc atggaggagc ttgacgaaag cggcgagggc
    11701 ataaacgcag ttttgcctct gcttagattt ctcgaaagac accccatggc tgatatcagc
    11761 atgacaagtg ccgtggtgag atatatggag cagttttacg gcagaggtta tgagaacatt
    11821 ctcatggact ccgttcgccg cagacctaca gtgcatactg tgagaatgtt aggcaggctc
    11881 agaaatgccg ccatagatga gggtcaggac ggtgaggagt ttgaggacgt gcttatagac
    11941 gttctcagca gtgaagcaga ggacgagata aaggataccg tcagggagct tatctgatga
    12001 aaactgagat gattcgtgtt tatggtatcg ttcagggcgt gggcttccga ccttttgtca
    12061 gccgtgaagc gtcagacctt ggactttgcg gcactgtggc aaacaaaggc tcttatgttg
    12121 agatacacgc acagggcagt gaaaaggctg tggacgagct gaaaaaagcc cttgaaagcc
    12181 gtccccctga gcgttcggtg ataatggaga taatttccgc ccatttggac gagccaccat
    12241 ttgacagctt tgagataatc gacagcgaaa aagaaaaagg tgatattttc gtatcccctg
    12301 acatcgctgt gtgcgaaaaa tgcaagggag agctttttga taaaacgaac agaagatatc
    12361 tccacccttt tataaactgc acccagtgcg gcccaaggct cactattatg gactctatgc
    12421 cctatgacag agttcgcacc acaatggctg actttccaat gtgcaaagac tgcgaggagg
    12481 agtacaccga ccctgccaca aggcgttatg acgcccagcc tgtgtgctgt aacaaatgcg
    12541 gtccggaggt ctatatcata ggcagtgaaa agaaaggcgc tgaggctatc accgccacaa
    12601 gagaagccgt catggcaggg aagataatcg ctgtcaaagg cataggcggt tttcaccttt
    12661 gctgtgacgc aaaaaacgag agcgcagtaa aaaggctcag agagcttaaa aaccgccctg
    12721 caaagccact tgccgttatg ctgaaggata tttctgccgc aaggcgtgag tgcgattttg
    12781 gcgaagtgca ggaaaagctt ctcacaggct ggcaaaagcc tatcgtgctt cttgataaaa
    12841 aaactagtgg aagcctttgc gaaagcgttg cacccgataa tcctacagtg ggagtaatgc
    12901 ttccctatgc ccctttgcac ctgcttcttt ttgattacga tgacggtgtg gaaatgaccg
    12961 acagccttgt tatgaccagc ggaaatgtcc gtggcgcacc tatttgtcga agcgacgagg
    13021 acgcactcag cgaaatagca ggcttctgcg acctgatatt atcccacaac agacgtatct
    13081 tgatacgctc agacgatact gtcatggaca catttgaggg caagccttac ataataaggc
    13141 gttcaagagg atatgcacca ttgcctgtta tgacatcatg cagtgataaa ggtcagaccc
    13201 ttgccatagg cggtgagctt aaaaatacgt tctgtatcag aaagggaagc ctttattatc
    13261 tctc
//
LOCUS       DAWEMR010000064        12974 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2016_length_12974_cov_10.348247,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000064 DAWEMR010000000
VERSION     DAWEMR010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12974)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12974)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12974
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2016_length_12974_cov_10.348247"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6641..6650
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7538..7547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3889 a         2452 c         3375 g         3238 t
ORIGIN      
        1 ttgatgtact atgttaagta ccaagcggat aacataaata ttattatatg aaaggacggg
       61 tatgctatga taaaaagcat gaccggtttc ggcagagagc atatcgtggc aaacggcaga
      121 gagataatcg ttgagatacg ctctgtaaac cacagatatt atgaatttac ttcgagaact
      181 ccgagagcct acggctatct tgatgaaaag ctgaaaggct ttcttaaaag cggtatctcc
      241 cgtggtaagg tagaggcgtc tgtttctatc tataatcagg agggtactga cgctgagata
      301 gagcttaaca aggctgttgc aaagggctat cttgacgcac tgagaggtgc ggctgacgag
      361 cttgaccttg atgacgatat cacactgtca catatcatga agcttcctga tgtgttcaca
      421 gttgtgaaga aaacagacga cgaagaagtt atctggaacg aggtcaagga cgtggctcag
      481 gtggcgttgg acagatttgt tcagatgcgt gaaactgagg gcgtcaagat gtatgacgat
      541 atcagcggca gacttgatta tatcgaggaa actgtgggca agatagaaga tcagtcacct
      601 agcgttactg aaagctacag agaaagactt tatgcaaaga taaaggaaac tctcggcgat
      661 aaggacatag acgagcagag gatacttaca gaagtggcta ttttcgccga taaggtggca
      721 atagacgagg aaacagtaag gctcagaagc catatctcac agttcagagg gcttatcaag
      781 tcggacgagc ctgtgggaag aaagcttgac ttccttgtgc aggagcttaa ccgtgaggtg
      841 aatactatcg gctcaaaggc taatgatctc actatcacaa aaatggtggt ggatcttaag
      901 gctgagattg aaaagatcag agagcagata cagaatatcg aataacggcg gtgtgatata
      961 tgcagcttat taatatcggt tttggaaata tggtgtcctc cgcaagggtc attgccatag
     1021 tttcacctga gtctgcgcct ataaagcgta tcattcagga agctaaggat aagggtactc
     1081 ttatagacgc cacctacggc agaagaacaa gagcggtgct tattatggac agcgaccatg
     1141 tggtgctgtc ggcagttgca cctgaaactg taggcggacg tgccgctgtt gatgaggagg
     1201 acgatgacaa tgtataagaa caatgcaaag ctgtttgtta tatcagctcc ctcaggctgt
     1261 ggcaagggaa ctatacttgc agaggtcttt aaaaacagag atgtgttcta ttctatatcc
     1321 tgcactacaa gaaagcccag agagggcgaa aaggacggcg ttcattatca ttttataagc
     1381 gatgacaact ttaagaaaat gatagacagc ggtgaatttc ttgaacacgc aggctatgtt
     1441 ggtcattatt acggaacacc aaagaagcct gttctcgcta atcttgagca gggcagagat
     1501 gttatacttg aaattgaaac taacggtgct tttcaggtga agaaagcctt tcctgaagct
     1561 gtgcttatgt ttatacttcc accgtctgtt aaggagatag acagacggct tagaaagcgt
     1621 ggaactgagt ctgacgatgt tattgctgac cgtgtaagtc aggctgccgg tgagatcggc
     1681 aaagccggcg gttatgatta tgtgataatg aacgacggac tagaggacgc tgtggaagat
     1741 tttgttgcag ttatgaactc cgtgaaagcc gaggacggca aggcggataa attcagatca
     1801 gaaaatgatg atacaaagaa attaattgat gaggtgctta attatgatgc ttagaccggc
     1861 cgtaagccag attttgaaga acaatgaaag ctattattct ctagttattg cagttgcaaa
     1921 gagagcaaga gagatcacaa gcgacgcttt tgacaacaag ctggtgcttg atgaaaagcc
     1981 agtaaagaca gcagttgacg agctttataa tggcgaatac aagataatcg aggatccaag
     2041 cctcagaaaa taatcgaggc tttttatggg gaggtgtacg gctgtgcgtg gagtttttat
     2101 cgtggcaaag gttgcaataa gccgtgcatc atacagcttt gacgcactgt atagctatag
     2161 tgtgccggag gagctggctc agaaagtgaa aagcggagtg cgtgtgcttg tgccttttgg
     2221 cagaggaaac cgcaaagccg tagggtttgt tgcgagaacg tatacggaag ccgagtataa
     2281 cgataagctg aagcctgtta tttcagtggt ggacggggaa agccttgtaa ccgaggaaat
     2341 gatgaggata ataatgtggc tgaaagaaaa cactttctgc acctattatg acgcttacaa
     2401 atccgttgtg cctacaggct tttcctacac tttttcacag cattacagcc ttgtcaatac
     2461 ctacattgac gaggatactt tgaatgaaga tgaaaaaaat gtggtggact ttctccgatg
     2521 tgcaaaaagt cagagggaga tagacagctt tcttgacgtg cataatgacg cacgaaagaa
     2581 gaaaactgtg gattctcttg tggataaggg ctatgttgag atcagcgacg ctttgaaaag
     2641 aaaagtcggt gatgagaatg tcagcatggt aaggctttct gacgagtatg tgtcagggga
     2701 gatacagacc actcttaccc cgaagcagac ccttgtggtg aagctgcttg aagagggcgg
     2761 ctgtgcctct gtgaaagaag tctgctatat gaccaactgc acttcaagca tattaaaaag
     2821 gcttgctgac aagaaagtta tcgtgcagta taaatacgag gtcatgcgtg acgccatagg
     2881 cgagcttggc gaaaggcaga atcctgacga tatcatatta aatgatgaac aatcagcggc
     2941 atatgagggc ataatggggc ttataaaagc tgaaaagccc gcaggtgcgt tgctttacgg
     3001 tgttacggga agcggtaaga cctctgtgtt cataaagctt ataaaaagcg ttatggatat
     3061 gggtaaaaca gcggtcatgc tcgttcctga aatatccctt acaccacaaa tgctcggcaa
     3121 attcaagtca cttttcggtg ataagatagc cgttatgcac tcgtcattgt cgttgggaca
     3181 gcgtactgac gagttcaaac gtgtaatgcg tggggaagca agaatagtta tcggcacaag
     3241 aagtgcaata tttgctcctg tcaccaacgt tggaataatc atcatggacg aagagggcga
     3301 gccgtcatat aagtcggact caacgcctag ataccatgca cgagatgttg ccatacagcg
     3361 gtgcggatat aataactgcg tgctgttgat ggcttctgca actccgtctt tggagagctt
     3421 ttattacgct caaaagggca gatatcactt gtttgaactt aaaaacagat attcaaagtc
     3481 gcccctgccg gcagttgaga tagtcgatat gcaggaggaa gcggcagagg gtaatgacag
     3541 cttgctcagc cgtgttatgt gtgataagct cacagaagtg cttgcgaaaa aagagcaggc
     3601 tatcctgctt ctgaacaggc gtggctatac aacgtatatc acttgcatgg actgccgaca
     3661 gcctgtggct tgccctaatt gcaatatacc cctgacctat cacaagaaaa atgatcggta
     3721 tatgtgccac tactgcggat ataccatgga taatatagag cactgtccac agtgcggctc
     3781 acagcggctc aaatcaagcg gtgtgggaac tcagcgtgtg gaggacgagc ttgaaaggct
     3841 ttttccgcag gcaaggcttc tgcgaatgga cgctgacacc acttcttcga gatattccta
     3901 tgaggagaat ttcaaggcgt ttgagaaagg cgagtatgat ataatgctcg gcactcaaat
     3961 gatagcaaag ggacttaatt tccctaacgt cacaatggtg ggtgtcatat cccttgacaa
     4021 ggcactgttc acaggggatt ttcgcagcta tgagagaaca ttttcactgc tcactcaggt
     4081 ggcaggaaga agcggcagag gagataaacc aggggtcgca tacattcaga cctttgtgcc
     4141 tgaccattat gtgctgaacc ttgcggcaga gcaaaattat gatgagtttt ataaagagga
     4201 gatagcactg agaaagtcgc tgacatatcc gcctttttgc gatatatgcg tcataggctt
     4261 ttcggcaccc cttgaaagca aggtaatagg cgcttcacgc tgggtgactc agcttataaa
     4321 agagtatatc tcacagcata gggtgaattt tcccctcaga gcgctaggcc ctgcaccatg
     4381 cacatttgaa atgataaata atcgctacag atacagacta atcctgaaaa cacgcaatac
     4441 tgcggatttt cgtgagatga taaagcacgt tctgggcgag ttctataaaa ataaagatta
     4501 tcagacagtt cgtatctatg ccgacataaa cggcgatatc gggctttaag aaaggatagt
     4561 taaataaatg gcactcagaa aaatacttaa taagagcgac gagattcttc acaaggtctg
     4621 caagcctgtt acagaatttg acgacaagct tggtcagctt cttgacgata tgacagaaac
     4681 actcaagaac gccaacggcg ttggtcttgc aggtccacag gtggcaaagc tgagaagagt
     4741 ggtcgttata ataatcgacg ataaggttta cgagcttatc aaccctgaga taacatggca
     4801 gagcgaggaa aagcagagag ttctggaggg ctgtctttca tgcccgaacg agtggggcta
     4861 tgttacaaga cctatgagag ttaagttcaa ggcgcaggac agacacggca agtggtatga
     4921 aatggaagta tcagagctgt ttgcacagtg cgtttgccat gagcttgcac accttgaggg
     4981 tcatctgttt actgagatag ttgaagaatt tgtagaggtc gaggactaat gaaaataatc
     5041 tttatgggta cgcctgattt tgccgtacca acgctgaaaa agctttatga cagcggatat
     5101 gaggtgcagg ctgttttcac tcagcctgat aagccaaagg gcagaggcta taagcttact
     5161 ccgccccctg taaaggtgct tgcgcaggag catgacacaa ctgtttatca gccgcaaagc
     5221 cttaaaaaag acggcgaggg gtatgttaaa attatcaatg accttgcccc tgattgtatc
     5281 gtggtggcgg cttatggcaa aatgctccct aagtcggtgc ttgatatacc aaggcttggc
     5341 tgtgtgaacg tccatggctc gctgcttcct aaatatcgtg gagcaggtcc gatacagtgg
     5401 gctgtgctta atgacgaagc tgaaactgga attacaacga tgcttatggg agagggcatg
     5461 gatacgggag atatccttgt ggaaaaggct actcctatcg gcgagaacga aacagccgca
     5521 gagctttttg acagacttgc tgaaatgggt gcagaggtgc ttgttgatac ccttgaaaag
     5581 ctcgataagg gtgagataac tcctgttaag caggacgaga gccttgcgac ttatgcacca
     5641 atgctctcaa aggagctttg ccctatagat ttcacaaagc cagtgagaaa gatacacaag
     5701 cagatatgcg gtctgtctga ttggccatgc gcaactacag tgcttgaggg caaaaggctg
     5761 aaagtatata gatctgagat agcttccaca aagccttgcg gaaagcctgc gggcactgtg
     5821 gtggataaca gcgacttttc cgttgcctgc tcagacggag ttataagatt tactcaggtg
     5881 caggctgagg gctcaaagag aatggatacc gctgattttc tcagaggcag acatatcgag
     5941 aacggcactg tgctgggata attcggagga actatgggaa acgccagaaa aacggtttta
     6001 aggctgctta caaggcttga aaacagcggc tcatattcaa atatacttct tgacgagggc
     6061 cttgaaaggt cagacctttc ccctcaggac aagaaattcg ccgcagcact tttctacggc
     6121 gttttggaaa gacagataac tcttgataac attattcggg aatattcacg caaccctaag
     6181 aataagcttg gtactgaggt cagagttata ctgagaatgg gtctttatca gcttttatat
     6241 atggactctg ttcctgacaa tgcggcggtg gacgagtctg tgaagcttgc aaagaaaaac
     6301 agaaatcctg tggcaagcgg ctttgtgaac gcaatgctaa gggagtttat aagaaacgac
     6361 aaaaagtttc caaagggcag aaatgccacg gaggagcttt ctatagaata ctccgcacct
     6421 gtatggctga cggagaagtg gacaagggaa tacggcaagg acatctgcct tgaaatgctg
     6481 aaaacctctg tgggacaggc acctgtcacc gcaagagtga acacggcctt tcattctctt
     6541 gaaagcacac tggatatgct cgccgaggag ggcattacct ttgaaaggct gtcagtcctg
     6601 cctgattgca taaggatatg cggtgcaggg gcggtggagc nnnnnnnnnn tggagcatac
     6661 taaggcttac aaagagggca ggatacacgt tcaggatctt tcgagccagc tttgctgtgc
     6721 ggctcttgat cctaaggaga acgatacagt tcttgacctt tgtgccgcac ccggtggaaa
     6781 gacctttacc attgccgagc gtatgaacaa caagggcaga gtgcttgctt ttgatctgca
     6841 taaaaatcgt gcagggctta ttgaaagcgg tgccaaaagg cttgggcttg actgcataag
     6901 ggcaggtgtc aacaacgcaa aggtctatga cgagaaaatg ccaaaggctg acaaagtgct
     6961 ttgcgacgca ccttgttccg gacttggcgt aataagaaga aaacctgaga taaagtataa
     7021 agaaccctca gatttcgaca gacttcctga aatacagtat gatatactga aaacgtcagc
     7081 ggagtatgta aaagtcggcg gaacgctggt gtattccacc tgcacgctgt caagggcgga
     7141 aaatgatgag gtggcagaca gatttttggc tgaacaccct gattttgagc ctgcaccgct
     7201 aggcgaggct ttcggcagcg acagcggtct ttgcagaatg tctatcacac ctgcaaaata
     7261 taattcagac ggatttttta ttgctaagtt tatcaggctg aggtgatctt ttggtagcat
     7321 ttgatgataa tggaaaaata gatatattgg ggcttctgcc tgacgagctg gaagccaaga
     7381 tactaaagct tggagaaaag aagttccgtg cagggcagat atttgactgg cttcatgtaa
     7441 agcgagtgtc aagttttgat gaaatgacta atctatctgt acaattaagg aatctgctca
     7501 atgaaaaatt ttgtttaaaa tcactatttg tgtcaaannn nnnnnnnagc ttgaggacgg
     7561 aaatcatgtg gaatccgtaa taatggaata caactacgga aacaccattt gcgtgtcaac
     7621 tcaggtgggt tgcaaaatgg gctgtcgctt ttgtgcgtca actattgcag gcttcaagcg
     7681 tgatctgact tcctcggaga ttctgggaca gatatacgct tctgagcgtg acagcggaag
     7741 aaagatatca ggcgtggtgc ttatgggaat aggcgagcct atggataact ttgataatgt
     7801 ggtgaatttc cttgaagtgc tgtcctgtcc aaagggcttt aatatgtcgt taaggcacgt
     7861 ttcagtgtct acctgcggca ttgtgccgag gatatatgag cttgcagaga aaaagcttgg
     7921 cataacattg tctatctctt tgcacgcttc caataataag agcaggagcg agataatgcc
     7981 cgttaatgac aggtatgata tcaacgagct tcttgccgcc tgccgatact attttaaggt
     8041 aacaggcagg agaatttctt ttgaatatgc tttgatagac gggcataacg attctatgga
     8101 ggacgcaagg gagcttgctg agcttctggg cggatttgtc tgtcatgtga atataatccc
     8161 tgttaataag ataaaggagc gtaattatca ttctgacaga aagtcagcca acagattcag
     8221 aggatatctg gagaagcttg ggataaacgc tactgtgagg cggaccttgg gtgcggatat
     8281 agacgctgcc tgcggtcagc taagacgaga atatgagatc tgataaaagg tggtgaatcg
     8341 cttgtttata gcgtctgata cagacatcgg aaaaaaacgc agtgaaaatc aggacagagt
     8401 gcatgctgaa tttctagctg acaatgttgc cgttgcggtg gtctgtgacg gtatgggcgg
     8461 agcgtcatca ggcggagttg ccagtcagct tgccattgac gcagtagtaa aacgtatcaa
     8521 ggaaaatttc agaaaagata tgaaaccaaa ttccataaga aatcttatgc tcacttcagt
     8581 gcattatgcg aatacgatcg tgtatggcaa gagcaaggag gatattgaca aaaacggtat
     8641 gggtactacc tgcgttgcgg cattggtggt ggataagacc gtctatatgg tgagcgtggg
     8701 cgacagccgt ggatatcttc ttaccaagga cggtatagct cagataacca ctgaccatac
     8761 ttatgtggaa atgctttata ggtcgggtca gataaccaaa gaagaagcga aaaatcatca
     8821 tatgagaaac gttatcacaa aggctgtggg cgttgaatca gatgttgagg tcgattattt
     8881 cgagcttgaa gaaaatgata attttgcggt gctgctttgc tctgacggtc tgaccaatta
     8941 ttgcacagac aatatgatat atgaaactgt gtttggcaat aatcttgacg aggctataaa
     9001 acagcttatt gctttcgcaa atgaatgcgg cggtaaagat aatattactg ctgcggtaat
     9061 agcaaattaa tggaatgaca ataaaacatg aataatatag gagttgaatt acatggattc
     9121 taatatcggc aaaaagctag acggcagata tgagataacc gaacttatcg gcgtgggcgg
     9181 aatggctgac gtttataagg cagttgacgt tatggaaaac cgtactgttg cggtaaagat
     9241 acttaagcct gagttttcac agaacgagga atttctaaga cgcttcagaa acgagagcaa
     9301 ggctatagct gtactttctc accctaatat cgtaaagata tatgacgtgg gattttctga
     9361 cgagatacag tttatcgtta tggagtatat cgacggcatc acccttaaag agtttatcga
     9421 gcagcagggc gtgcttaggt ggaaagacgc tttgcacttt gtaactcaga tactccgtgc
     9481 tttgcagcac gctcatgaca agggtatcgt tcacagagat atcaaaccgc agaacataat
     9541 gcttttccct gacggaacta tcaaggtcat ggattttggt atcgcaagat tttcaaggat
     9601 cgacggaaag acactttctg acaagactat cggttctgtt cactatatct cacctgagca
     9661 ggcaaggggc gatatgacag atgaaagatc tgacatatac tctgtaggcg ttatgctcta
     9721 tgagatgctc acaggcagaa agccttttga cggcgaaaat cctgtggcta tcgctctcaa
     9781 gcatatgcag gaggatccgg ttccgccaag agagatcatg ccttctatcc cagaggctct
     9841 tgaagaaata gttatgcacg ctatggagag agatcctgca agacgttatc agtcagcagc
     9901 agagatgata aaggatatag acacattcaa gctcaatcca tctgttgttt tcggatataa
     9961 gaacagtcct gttcctatcc ctgtggctga taacaataac aaatattttg acgatgagca
    10021 tagtgagaac tatgacgacg atgaagaata tgaggacgat gacgaagaaa acgatgacga
    10081 ggaagaagag gaagaaaaga agcgttctta cgttgttcca atactccttg ctgtgacagt
    10141 tgcggtagtt atcattgcag cgttcgttat cgtaggagtt gtattcaagt cattcagcgg
    10201 ttcaaacggt atgcacacag gcacagttac actgccaaac tttgtgggca agaatatcgt
    10261 tcaggttgag caggattata aggataagct taaatttgat atcaccgaag agtataatga
    10321 ccagtatgac gagggaataa tcacattcca gggtcaggca gcaggaaagt ctgttaagga
    10381 aggcttcacg gtcacactta ctgtaagtaa gggcaaaaaa atggcaacta tccctgacat
    10441 ggtgaagaaa gacggcactg tggctgaaag cgagcttaag gctgcaggat ttatggtcgt
    10501 taagagagat atatggtctg aggatatagc ttcaggtctt gttgtgaaaa cacagccggc
    10561 agcaggtgag cagtatgccg taggtgcgac tgttgctttg tttgtaagta agggccctgt
    10621 tgaaacaaga gtcaaagtac caagcgttgt aggtcttaca gaagaaaagg cagttgcaat
    10681 gctcaaggag aacaagctca aggctgaggt caaggagatc gagcatgagg gcgataaggg
    10741 caaggtcatc gagcaggata cagatgaagg cacatacgtt gagcgtgata caactgttac
    10801 tatttacgtt tcgaccggag aaacttcacc tgttgaggta acattctcac tgccactccc
    10861 aagtggtatc cacggctcat attctataga catctacaat aacggcaacg ttgcatatac
    10921 acagaagatc ggtagtggag aatctgttgc aggcggttct gtaaatgtaa ctgtaaaggg
    10981 caagaagaca gaaactctga ctatcttcat cacaaatgac gaaaatcagc agaagtgccg
    11041 ttatgcagta tatgacatag attatgataa gcagacagct actcttagcg gaagcctgaa
    11101 tgaaagcggt ctgatcgaca ttactccgac aacgaccaca actactacaa ctacgaccac
    11161 aacaactact acaactacca ctactgaagc acctgcaccg gagcctcagg agccagacga
    11221 gcagggtcag tagttcatga atggaataat cgttaaagct atcggaggca tctactatgt
    11281 agaagcctcc gacggcattt atgaatgtaa ggcaagagga gtattcagaa gaaagaatat
    11341 ttctcctgta ggcggcgaca gagttgaatt ttcattggag gacgacggaa gcggagttat
    11401 cgagaagata gaggaaagac gctcattgct tataagaccg cctcttgcaa atcttgatat
    11461 gcttgtgttc gtttcgtcta cctgcgaacc aagacctaat ctgctgttgc ttgataagtt
    11521 tattgcaata gctgttttca aaaaaataga gcctgtggta gtctttacaa agatagacaa
    11581 ggaaagctgt gacgagcttg tggacatata caagggtgca gggataaaga cctttcaggt
    11641 agacaatacc accggcgagg gcagtgaagc tgttaaggca gtgttggagg gcaagctgtc
    11701 ggcatttaca ggaaacacgg gcgttggcaa atcgtcactg ctcaataata tgttccctgc
    11761 attggggctt gccaccaacg agataagcaa aaagcttggc agaggaaagc acaccacacg
    11821 ccatgtagag ctttacaagc tggagggcgg tggttatatt gccgataccc ctggcttttc
    11881 gtcgtttgat accaacagat atgatattat tttcaaggac aagcttgcag gttgttttcc
    11941 tgagtttcag aaatacgagg gcaagtgcag atttcctgat tgcagccaca caaaggaaaa
    12001 gggctgtgcg gtcatagagg cggtaaaaaa tggcgagatt gcaaagtcaa ggcatgaaag
    12061 ttatgtggaa atgtacgagc aggcaaagca gcttaaggag tgggaataca aaaatgacta
    12121 agaaatgcac tatcttttca ggcggtgaag cggtgtcggc tcagtgtgtg gcagaatata
    12181 agccttttat cgacgattct cttgtgatat ccgctgacaa gggctataag cttgcacagg
    12241 cagtgggcgt aaagtcggac attattatgg gcgactttga ttccgctgaa aaacctgttt
    12301 ttgataatat cgaggtcttc ccctcagaaa aggacgatac cgaccttatg cttgccataa
    12361 agcagggctt tgagtcgggt tgtgatgatt ttctcatatt cggtgcaaca ggcggacgag
    12421 tagaccactt acttgggaat atccaatctc tgggatatat tttatcttgt ggcggtgaag
    12481 gaatgatagc cgcagatagg gagataattc ggctttatcg tgcaggcagt tacaggatat
    12541 atgaccgtga gggcttttcg ttctcactgg tggcatatac cgataaggta gagggtctga
    12601 ccataaaggg tgcgaaatat gaagtagagg actgtctgct gaccaatgct ttcccacttg
    12661 gcatatcaaa cgtggtgaaa ggctttgatg ggcaggatag aaaatacgct gagatatcat
    12721 tcaaaagtgg ctatctgctg tcagtacagt caaaaatggt aaccgatttt tgatttttga
    12781 ataatataaa ctataactat tcgggaggta atacttatga aagttgtagt tgtaaaaagt
    12841 ccaaaggtca tatcgggttt aatgaggctt attttcaaga tcaaaaagga agaagaatag
    12901 gataactggc ggtactcgtg tgatataatc cccttagagt agacacatga aataataaaa
    12961 tggtgtaact ctaa
//
LOCUS       DAWEMR010000065        12952 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2021_length_12952_cov_10.802667,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000065 DAWEMR010000000
VERSION     DAWEMR010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12952)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2021_length_12952_cov_10.802667"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2522..2531
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3608 a         2670 c         3050 g         3614 t
ORIGIN      
        1 gtttactgta gtataatttc attagaaaat acagaaaacg tttaagttca gttacgaggt
       61 atatatttat agtgggtata atggaggtca cgctgatgaa aagcattaat tttatgggcg
      121 acaatggttt caaaaaagca cttgagagcg ttggtgtaac tgcaagcagc agtatcagca
      181 gcgagaagat ctcgggctgt tttaaatatg ttacggctga cgatatgtgt attatcactg
      241 gatactgcgg tgacagccat atcgaagtgc tggagcttcc ttttgagctt cgtggcaaaa
      301 aggtcattgc tgttgcaggc ggtgctttcg cacatcttta cgaccttaaa tgtgctgtta
      361 tatcaaagga cgttgttatc atcggaagaa aggcatttta caaatgctgt tcactggtga
      421 atgtgactat acctgagagc gtaagattta ttggtgagat ggcattctgc ggttgtgaat
      481 ctctgagatt tgcgactata cctgatggtg tggaaaggat actgccattc acattctaca
      541 attgcgacag tttggagaga gctgacctgc ctgagagcgt aagagagatc ggtgagtctg
      601 cattcggttt ctgctcttca atgaaagaaa tttgtcttaa tgaaaatata gtttccatag
      661 gaaacggagc tttccagaac tgcatttctc tgagagagct tgaaataccg acagctatga
      721 tcgagatcgc agagaatgct ttcacattct gctcaggtct ggaagcgatc tctataccat
      781 ttggaatcga ggctgttggc gactatgcat tcttcggctg ctctgctctg aaaaaggttg
      841 agttcgcagg atctgtaaga aagataggcg agtgtgcatt tgcactttgt gagaagctcg
      901 agagcgttta tcttcctgat tccgttagca ggataggcaa gagagcgttt gctcagaata
      961 ctgtagttaa gacggtgaga atatcaggca agatcgctta tgttggaagc gaggctttcc
     1021 gtggctgtaa tgctttgaga gaagttgtta tcccatcatc tgtaaagaag cttggcgcaa
     1081 aggctttcgt ggattgctct gagttacgaa gcgttgttat tcagcatggt gccgatggca
     1141 ttgcggagga cgcttttgag agaggtgccg ccttgattac tgctgacgct gttcctgcac
     1201 ttgatgacga aacattcagg atagccgagg ctgtttacaa caaaaatttc gctttataaa
     1261 atatataacc tctggatcgg ggcggcagct tttaagttgc cgtccttttt ctgtctattt
     1321 gcaggtggga tatatttcaa gtcggtagga tataggtcat tgtcgtaggt acataaaaaa
     1381 cgagcgacca aaggtcgccc tacgatatgt aaacaaaaaa aaaatacgct ctgcataatg
     1441 cagaacgtat tcagtggggt gggtagtgag attcgaactc acggtcttca gtgccacaaa
     1501 ctgacgcgtt aggccagcta cgctataccc accatacata ttagaagaaa atactcgcag
     1561 cagattttca tctggaacga gtttaatggt acgccatact ggattcgaac cagtgaccta
     1621 ccgcttagaa ggcggttgct ctatccaact gagctaatga cgcatactgt actcattgat
     1681 atctcaaact cggataaaag tttaagatat attggagcgg gtgatgggaa tcgaacccac
     1741 atgaccagct tggaaggctg gagttctacc actgaactac acccgcatac tgcctgacca
     1801 ggcgtttttg gtggtctttc aagcttaccg ctcaatcaac gatatttatt ataccactac
     1861 aagagccttt tgtcaagcac ttttttatat ttttttcatc taaactcaac tttgtgcaat
     1921 ttcacttatt tgttcttttt atgttgtttc attttattca ctataacatc tttgaacaaa
     1981 aagccaacga tacctgctac gagagctact ccaagcacca caagtgcaac tgtgaagttg
     2041 ccgcttttta cattgctccc caaaatcgtg gagagcatta ttgccggaag tctgcaaggc
     2101 atgacataat agaaaaaatc cttcttgctt atgggagtca gggggacgat atatgagata
     2161 acgtccttgg gtattcccgg tatgagatag aggattataa ggataacggt tagctttttc
     2221 tcattttgca ggaatttgaa tttttttagc tgtttctcgt caacaaaggc ttccactatt
     2281 ggtctgccaa attttttcac aagcacgaat attgccattg tgccaaggag cgtacctaag
     2341 aatgatagca acccgcccca gaaccagccg aacagcactc cacccaccac ttgtacaggc
     2401 ggtatgaccg ctatgaccac ttgaagagct tgtatgaaag taaagataag cactcccaca
     2461 atgccactgt agttatcaag cttacgctcc aaggctgcaa tgcctgcctt gctgtgaagc
     2521 gnnnnnnnnn ngtgaagcgc ttttataaga ggtatacacg ccactatagc cattgctatg
     2581 accgctatta ttgcaacaag gcttattatc tgtatttttt tcttgtgcct tgcagtttgt
     2641 attttcgcct cgtttaatat ttctttctct tgttcttcgt tcataaaaac gtcaccgtca
     2701 ttttcttctg agtatttttt ttctaaacca atttatgtac atatctgtca actgtgtgag
     2761 ggcatagtca taaataaaga aaaacaggtt tgccgccgcc cacagaacaa gaacggcata
     2821 cttgccaaac atctccatgc cctcgagcat atccaccgaa gtaaagatat actgaaaagc
     2881 caaaaagaat atgactatcg ccacattgaa cactgcaagc tttattgccc acaccaacag
     2941 gcggtttttc ttttggtcga gataatattt aagtattgga taatacccca taaacaatat
     3001 gaaaagcagg gtcgcttcgt aatttggggt cacgaaaatg ctcagcaggc tcactgcaca
     3061 ataggtggct atcgcccagt ttttatccac ctccactatc atcacaacca tcatgaagcc
     3121 tgcgaaggta gggatagtat aatcgagcat tggcaaaaag ctgcttgaaa acatcaaaag
     3181 caggcagaca gcagagcata tgccgccaag tgccactcga aaagctattt tatttctcat
     3241 gattatttaa cagcaaggga taaggtcgcc gcccatcatt tcacagcagc agtctgcaca
     3301 gcaaagggag gaacagcagt caggtccgct catgccgcct gcttggtagc cctgagttgg
     3361 gttgccgctc atatagccgc ttcgctgtct gttcatctgg gctaacacgc tttggtactc
     3421 catgttattt gggtcgagct ggacggctct tgtgaagttg ttataagcct cattgagcca
     3481 gcctctgcgg tggcagatag tgcctttgag gaaataccac tcggcatttc tttccgagga
     3541 ggacacatta tcaagtatct ggtctgcctg agtatagctt ccgctttgga taaggcttct
     3601 gatatagcct gagtcaaagc cctgcgaaga ggcatagccg tcatagccgc tttctgcgga
     3661 ctggtatcct ccgccacgtc ttgaggtcat tatctgatcg taggcggcgt ttatctcgga
     3721 cattttctcc tcagctacgt cagctagggg gttatcctga tactggtcgg ggtgatattt
     3781 tttcacaagc tctttataag ctgattttat ttcagcgtct gaagctgttg gggatacgcc
     3841 taaaatttta taagggtctt tggagatcat tgatcgttat ttcctttcct gttattgcgg
     3901 aattttccgc ttcttatttg agtatagaca tttttaagtc caagatacac gatattatca
     3961 agtatatcac gatatcgctc aagatcgagc ttcacataga tatctgccat ttcgcccagt
     4021 gtgaaattta tgctgtcatc acaaaaggcg tggacttttt tgagatagtc cgcatttatg
     4081 ccctcggtgg gtggaaattt tgcaaggagc ggattgtagc tttcactctc gtggtcgtcg
     4141 ggcaggtcgt caagagcgtc gcagatatag ataaatctgc cgagaaggta gccgaatcgt
     4201 tcaaggagcg gtcggcgttc gtcctgcctg gtgcaaagat tccctgcaac ggcttgcatc
     4261 ataagtgcgg taggctcgca ggcaaggtcg atgcctgctg ttttttcacg ttctatttta
     4321 ttttgcaatt ccattgcgtt ttcaacggca ggggcaagat ccggatagag gcaagccgct
     4381 tttttatagg gctttttgaa aaaaggcaga agtgcaaatg cggcaagctt gccttttagc
     4441 cctttatcag ctatatcgtc acggagctta tgatacgtga gtatcacggc ggctgaggac
     4501 gtatatttaa gattgcaagt acactttgcg cacattgttt ttttgaaagg gtgtgcgata
     4561 cagcgttgct tttctgcgtc aagcttcact cccctcacag agagatccat aagggagaga
     4621 aatgcgaaat cgtagctaag ggtaaagcgt gtcacaaagc catagcgcct gcccatatcc
     4681 ttacaaagtc cgcagtagac agctttatag atatcatatt caaacattcg catatagggt
     4741 ttgaaagggc ggacatatcc gaacataggc actccttagg ttttcgttac tatacattat
     4801 aaaacacgat tgtgtatatc ccgtgaaata tataacatta tacaacaatt ttaagataaa
     4861 agtcaataat ttttatgata aaaataaggg tggggtaatg gagaaatcga aaagacggac
     4921 gggataaaaa tagggcaaaa tacaaaaggg cgaccaaagg ccgcccctac atcaggtttt
     4981 tataaacaaa cgccgtgatc aggtcacagc aaactgtgag ttatagagat cggcatagaa
     5041 tccgttcttt gccataagct catcatgact tccctgctct actatgtgtc ccgaatccat
     5101 tactatgata gtatctgcgt tcttgatagt agaaagtcta tgagcgatta taaagctcgt
     5161 tctgccctgc atcattttct caaaggcacg ctgtaccaat atctccgtac gggtatctat
     5221 agacgatgta gcttcgtcca gaatgagcat tggcggcagg gagagcatta ctcgtgcgat
     5281 acagagaagc tgtttctgtc ctgcagagat agtgctgtag ctgtctgaaa taacggtatc
     5341 atagcccttt ggcagacgct ttataaagcc gtgacaatgg gctgacttgg cggcggctat
     5401 catttcttcc tcggtggcgt caggcttgcc gtagcagagg ttctctctta tggtgcctgt
     5461 tttcagccac gtttcctgca agaccatgcc gtacatactt ctcatggagt cacgggtaca
     5521 atcctttata ggctcgccgc ttatgcgtat ctcgccgcta tcggtatcat aaaatctcat
     5581 aaggaggttt atcatggtgg tcttgccgca gcctgttggt cctacgatag ctgtacgctg
     5641 accgctcttt acatggaggt taaaattctc gataagcttc acatcaggca catagctaaa
     5701 tgacacgttg tcaacgtcta atgttccgtc aacggaggta aggacagtag cgttagagct
     5761 gtcaggtatc tcctctgttt cgtctatgac ctcaaagaca cgctttgcag aagcgaatgc
     5821 actttgaagc tcggtgataa cgcctgatat ttcattgaaa ggctttgtat actgatttgc
     5881 ataggtgagg aaacagctaa ggttaccaac tgtgaagccc acgcctttta ttacagagag
     5941 tgcgcccacg ataccgacag ccatgtagac gaggttgttt acaaatcgtg tgcatgggtt
     6001 ggtgagtgag gagaagaatg tggcgttaag tcccaccttc tggagtttca ggttcacttc
     6061 ttcaaactgc tcctcagctt cttcctgata accgaatgct gttactacct tctgattgcc
     6121 tgtcatttcc tcaacaaggc tggtcatgtc gcctcttgca actgattgct ggtggaaaag
     6181 cttgaatgtg ttcttggcaa taaatcttgc gacaaatagg gaaagcggag tgaggataac
     6241 cacaataagg gttattttca cgtttatgga gagcatgaag ccaagtgtac caaaaattgt
     6301 acatacgcct gtgaagagct gtgaaaagcc catgagaagt ccgtctgaca cctgctccaa
     6361 gtcggagatt atacggctga ttatatcacc gtgagggtgg gagtcgatat aagaaagggg
     6421 aagcttttgg agcttagcga aagcatcgtt tcggatatct cttaccacgc aataggttat
     6481 cttatttgta caaagtgaca tgagccactg ggcaatgcct gttacggtga ctgttacgcc
     6541 aagcttgatg atgatattga taattccgtc aaaatcaaca aagcccattt ctacgataag
     6601 gtcaacggcg ttaccaacga ggataggagc ataaagtgtg gtggcaacgg ttatgactgc
     6661 aaagaccatg gacaggacga acagccacat atgcggtttt gcgtatttga gaagcggtct
     6721 gagagccgag gtatcaagct tgttttttgt gctttgctta gccatttgcc tgcgcctcct
     6781 tttctgaaag ctgtgaaagg catatttctt tatatacctc gcagccctta taaagctctg
     6841 aatgagtgcc gatacctgct attttgccat cgtcaagaac gattatctta tcggcacttt
     6901 ttatagatga gattctctgt gaaactatga aaacggtcat gccgcttgtt ttttcgtgga
     6961 tagcttttct tagtcttgca tctgtggcaa ggtcaagggc ggaggctgaa tcatcgagaa
     7021 tgagtatctc aggatcacca accaatgctc ttgcaatggt aagtctttga cgctgaccgc
     7081 ctgagaggtt tttgccctcc tgcaagatca tttcgtcaag cttatcaggc tttttctcaa
     7141 caaagtcttt tgcctgagcg atatcaagag cgttccagat atcctcgtcc gtggcgtttt
     7201 tatcacgcca ctgcatattg tgtcttatag tacccttaaa gaggacagct ttctgaggaa
     7261 caatgcctat catacctctg agctgtgaga atgggtactc ttttacgtca acgccgtcaa
     7321 cgaggacttc cccctgacgg acgtcataaa atcttgggat aaggttgata agagaggtct
     7381 tacctgagcc tgtaccaccg attataccga tagtttcgcc tttcatggct ttgaagctta
     7441 tatccgaaag agagtcggct tcggctgatg cgtatgagaa gaaacagttc ctaaactcta
     7501 cgcatggggc attaccgtca gttgcgacag gcttattatc cttatcggat acacttggct
     7561 ccacagcgaa aacgtcgttt attctgatag cggaagcctg cattttggtg atatttgtaa
     7621 cgagtatcgc catagcaagg agtgccaaga gtatctggtt catatagttt acaagggcta
     7681 tcacttcgcc ctgtgagata gagcctgaat aaactgtttt tccgccaaac caaatgatag
     7741 cgatgatagc gaagtttacg ataacatagg tggcagggtt cataagggcg gaaattcttc
     7801 ctgcaattat ctgctgacgt ctaagttctt cggtattatc agcgaactct ttttcctcgt
     7861 ctttctgacg ggaaaaggct ctgataactc ttgcgcctgt gagagtttcg cttgtcataa
     7921 ggttagtttt atccagcttt ttctggatat tcttatactg cgggatagtg atgtgcatta
     7981 taagatagat gacataggca aggcaaggtg cgattatcag gaagataagg gtaagcttca
     8041 ctgagataga gaatgccatt atgattgagc ctaaaacgat aaaaggcgaa cgaagaaaca
     8101 gacggaggag aatattcacg ccctgctgag cggtattgat atccgctgtc attcttgtga
     8161 cgagggtatg gcttccaacg ctgtctattt ccgcatagga cagtgaattg atatgcttga
     8221 agaggtcacg tcggagggct gttccaaagc ccagggacgc ttttgcggcg aaatactgag
     8281 cagtcagcgc acaagccaga ccacagaagg cgaggagaac aagcacgcca cccattttat
     8341 agatgtaggg cttatcttga ttttttatac ccacgtcaat gatattagcc ataacgagtg
     8401 gcactgcaag ctcgaagcag gcttcgataa gcttaaacag cggaccgatt atgctttctt
     8461 ttttatagtt tttcagatac ctaaaaagct tcagcatttt tctttctccc aactaaataa
     8521 aatcatataa attttattat atcatatgtt tttgtacatt tgtagacgaa taagttaatt
     8581 ctttaaaaaa gtgggggata atgtaggggc aaaaagttcg cccatacaat aatcatcgcc
     8641 gcaggcgata ccttcattcc tcattcctta ctcctcactc ctcacttcta aaaagggggg
     8701 ttgacaaaag ggtgaaagtg tgctatagtc atatcaatag gtttaaatgc tatgacggaa
     8761 ataagtagcc ttttgcgaaa tttcagagag ctttcggtcg gtgcgaggaa gcatttctac
     8821 aaagtgtgaa tacattcctg agctgttcgc tgagaaagct tttgcgtaag gcgaaacggt
     8881 ttgcacacgt tacagtgcta tggcttcgct ttctgctgag cctgtaaggt cgggagtttc
     8941 cccgataaac tgaggtggta ccacgataat tcgtcctctg acagatattc tgtcagggga
     9001 ttttttattt atgtaccgca aaaaataata cggaggtatg aaaaatgaca gtatttgaag
     9061 aactgaaaag aagaggtctt ctcgctcagc ttactgatga ggaggaaata aaggagctta
     9121 tcaacgctgg caaggcgact ttctacatcg gctttgaccc gaccgctgat tcactccacg
     9181 ttggtcactt tatggcactt tgccttatga agcgtttgca aatggcaggc aacaagccta
     9241 tcgttcttat aggcggcggt acagctatga taggcgaccc gtcaggcaaa acagatatga
     9301 gaaaaatgat gacaaaggaa actatcaacc acaatgttga atgtttcaaa aagcagatgt
     9361 cacacttcat tgatttttct gatgacaaag ctattatcgt aaacaatggc gattggctgc
     9421 ttgaccttaa ttatgtggac gtacttcgtg acgttggcgc ttgcttctct gtaaacaaga
     9481 tgcttacatt cgagtgctac aagcagagaa tggaaagggg tcttactttc cttgaattta
     9541 actacatgat aatgcagtca tacgacttct acagactttt ccaggattac ggctgtaata
     9601 tgcagttcgg tggtgacgat cagtgggcta atatgctggg cggcactgag cttatcagaa
     9661 agaagcttgg caaggacgct tatgcaatga ctatcactct gcttcttaac tctgagggca
     9721 agaaaatggg caagacagaa aagggcgctg tatggctcga ccctgaaaaa acttctccat
     9781 acgagttcta tcagtactgg agaaacgttg gtgacgccga tgttatcaag tgcctgaaga
     9841 tgctcacatt cgttcctatc gaggagatag agataatgga aaaacacatg gagggtgctg
     9901 aatacaacaa ggctaaggag cttttggctt acgagctgac aaagcttgtt cacggcaagg
     9961 aagaggctga caaggctgac gctgcggcaa aggctatttt tgcaggcggc ggaaacagcg
    10021 acgatatgcc tagcacgact attgcgacag ctgatcttac tgacggcaag ataggtattc
    10081 ttacgcttct cgttaaatgc ggactttgtc cttcaaacgg cgaggctaga cgtcttgtta
    10141 cacagaacgg tattgcagtg aatggcgaga agttcactga ccctaagggt cttgttgatc
    10201 tctctgagcc tgttgttatc aaaaagggta agaagatctt ccacaaggct attgcggaat
    10261 aatatagtgc aaaataattt ccacgggtgg acagaagttc acccgttttt tgtatacaaa
    10321 tatatggtat tttgcaatat tcataaacat tcacacaaaa tacatataag gtttagctat
    10381 atttaaagtt attcatatat acttttaatc aaaggaaagt ttacacgaag catacgaaaa
    10441 aatcacgaaa agaggcgtgg atatgaatgg caatcaggaa gtgttcagac ggatagagaa
    10501 aaaatatatc gttgatgaac cgacttatga aaagcttata aaaaagctcg ataggcattt
    10561 tgtcaaagac agatattaca aaagcactat ctgcaacata tattatgaca caccaagtca
    10621 tcagcttgtg agaaactcca ttgaaaagcc tgtttacaag gaaaagctga gagtgaggag
    10681 ctacggcgta cctaatgatg aagacatggt ctttgtggag cttaaaaaga aatacaaggg
    10741 tgtggtctac aagagacgta ttgaaatgac ccttgcacag acccgtgatt tctttgcagg
    10801 caaagaggta cctcatgata atccgcagat agagaacgag ctgaaatatt ttctcaaatt
    10861 ctatgagggc attgctcctg caatgtatct gagttatgac aggcttgctt actgtggtgc
    10921 ggaagacccg tcccttaggc tgacctttga cacgcacatt ctttaccgtg aggagcaaaa
    10981 ggagcttgac aatggcattt ggggcaaaga gcttctgcct gctggcatga gggtaatgga
    11041 gataaagata ccgaacgcta tgccactttg gttatcggct atacttgatg aactggagat
    11101 atatcctgcg tcattctcaa aatacggaac tgcgtatctt aacgaatttt ctgaaaaaat
    11161 acataaaggt aaggtgataa gctgtgctta attcaactac tcttgcggct actactgtga
    11221 ccaccactga aacgaccgtg gcggcgataa ctacaaacga atttgttttc tgcactgcgg
    11281 cttctttggt gcttggtctt ataatagcgt ttgcattcaa cttcaagcaa aagaaaacaa
    11341 agagctttat gatgacgctt gctttgctgc ctgtgatagt acaggtagtg ataatgcttg
    11401 taaacggaaa catcggtact ggtgtggctg ttatgggtgc attcagcttg gtaagattcc
    11461 gttctgtacc gggaagtgcc atggacatat gcgctatatt tctttcaatg gctgtcggtc
    11521 ttgcgacggg cacgaaccac cttgcgttgg ctcttatgct tgctgtgtgc gtgagcctgc
    11581 tgaacattat ttacacgctt ctgcctattg gcaaaaagaa gtcgggtcac aaggatctta
    11641 gtatcactat ccctgaaagc cttgattaca cggaggtatt tgacgatata tttgaaaagt
    11701 atctttcaga atgtgagctt gtttcggtaa agaccacaaa tatgggaagc cttttcaagc
    11761 tgagatatcg catagagcta aagggtgatg ctaacgaaaa gaagcttatt gacgaactta
    11821 gatgcagaaa cggcaacctt gagataatgt gtgcagtggc agcagacatt gaggaacaat
    11881 cattctgaca aaataacaaa agtatccata agcaaaaccg cacaaggcta aagcggtctt
    11941 gtgcggtttt tttcaacctt ttttaccata caagggggta tttttcatga aaatttcgga
    12001 cactaaaaag ctggcagcta tcacggcggc gttttttatc acggtcacgg ctttttcagg
    12061 ctgttcagag agtgcaaatt cttcacagac cacaagcagt gtttctcagg cggcaaagtc
    12121 tgacattgac gcaagcattg cggcagagga cattgacgtt ggatatgacg aggaaagctc
    12181 agtagggatt acatttgctg atggcagtgc ggctatagac ggtgaaggtg cgacatcaga
    12241 aggggaaact gttaccattt cgcaggcagg cacttacatt ctttcaggca cggcgaccaa
    12301 cggcaggata atcgtttcag cggacaaaaa ggctgaggtc aagcttattt tcaatggagt
    12361 tgacataact tgtgctgaca acgctcccct gctcgtgagc aaagctaaaa aagtttatat
    12421 cgtgctggag gacggcacag agaacgtttt gacggacagt gcaagctact ctttaggaga
    12481 agatgacagc aacacagacg gtgctatttt ctccaaagcc gaccttacta taaacggcag
    12541 cggtacactt actgtgaatg cgaactacaa gcacggcatt gtttcaaagg acgatcttgt
    12601 tatcactgac ggcaatatca atgtgacctc ggcatcaacc gctatggagg gcaaggacag
    12661 cgttaagata tctggcggaa cattcaacat ttccgcaggc acgaacggca taaaatccac
    12721 taacacagag gcttctgaca aaggctttat aagcgtgaca gggggaagct tcacagtagt
    12781 cacaaacaat gacgcattcg aggcggaaac tgtgctttct atcgagagcg gaagctttga
    12841 catcaccaca ggcggtggaa gtgcaaacgc ttctatgaag tctgacggca cgccaaacag
    12901 aaattggcaa agcaacatga gcaacggtgg tggtggtccg aacggaatgg gc
//
LOCUS       DAWEMR010000066        12911 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2034_length_12911_cov_15.167393,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000066 DAWEMR010000000
VERSION     DAWEMR010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12911
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_12911_cov_15.167393"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3715 a         2639 c         3259 g         3298 t
ORIGIN      
        1 ttgcagttgt agttgctaca ggatatatca taggtatatc gaaagaaaag tttcgcggag
       61 taaattattt tcaaaaagca aaatgaaagt taaacaagaa aatcctgcaa agcgcagctg
      121 taaatatagg aggaatgtaa agtgaaaagg ttcagattta taatcaaacg gctgctttca
      181 atgatagtca taatgttttt ggtatccttt ctggtgttcg ttgttctgag aatgtcagac
      241 gcagaccctc tcacagtaat gataggaaac aatcaatcca ctcctgaact caggcaggct
      301 cttacaaaaa aatatcacct tgacgaccct gtgcatatac agtatctcag gtggataaag
      361 ggagttcttt caggcgactt cggcacagac tatgttgacg gtcaggacgt aaaaggtctt
      421 ataatgtcaa ggcttccaat aacattcgga cttgtgataa tgagttccgt cataggctca
      481 gtgatagctg ttataatggg agttgcagcg gcgttgagaa aaggcagacc ctcagacgca
      541 gcaatttcgg gacttatgct tgtgctttca ggtgcgccgt catttttggt aagcatactg
      601 atacttattt tcatgacaaa gtacgttcct ggatattcgt ttatcggaac atatacaaat
      661 ttcggagagt tcttacagcg tataacgata ccgagcctga taatggcgct gacgttcgtt
      721 gcgatgcttg gacgtatcac acgaagcaac atgataaatc agcttcaatc cccttatatc
      781 gcaacggcgg ctgcaaaggg attaaagcaa agcaacataa cctacaagca cgcttttcac
      841 aacgccgtta ttcctgttat aacagttgca ggatacatga tagcaggctc tataggctcg
      901 acggtaatag ttgagcaggt cttttctctt cccggtatag gcggtctgct catatcggcg
      961 atagaggaaa acaattttcc tatagttcag atacttgtgc ttttcatgct tggagcttat
     1021 cttgtaatga gttttgttat cgacatactt tataccgttc ttgatccgag agttgatctt
     1081 aaataaggtg ggatttttaa tgaagaagaa aaataaagca ggaacgtttt tcacacctgt
     1141 tgtggcggtg acgggaatat tccttgcggt aatagttttc gtatcgctct ttgcaagcgt
     1201 tatcgcaccg tatgaccctc ttgccacaga cgtgaatgcc atatatgctc cagccttttc
     1261 tgagggacac attttcggaa ctgacggact tggcagagat cttttttcaa ggcttatatg
     1321 cagtgcaaga acttccattt tgaatgcctt tcttatagtt gccatagagg tagtcatagg
     1381 tgttcctctt gggcttatct gcggatacta cagaggtcct cttgacagta tcgtaatgag
     1441 ggtgtgggac gttataagtg caataccgcc acttcttctt tcctttatcc ttattgcagt
     1501 aatgggaaag ggcatgatga caggtatcat cgccataggc atttcttata ttcctcttac
     1561 aacaaagacc gcaagatcaa tgataatgac agagaaaaag gcggtgtatg ttgaagcctg
     1621 caaatcaatg ggcttttcag acgcaaggat aattttcgtg catatcctgc cgaacatcat
     1681 cacacctatg atatcgcagt tcacacttga tattggtaca gctatcacat caatggttac
     1741 tctgagcttt ctgggacttg gcgttcaggc tccgaatgcc gactggggaa cgcttctccg
     1801 tgacggcatg ggcaaccttt ataatagctc ggcactgctt tttatacctg caatatgcgt
     1861 aatacttgta acagtatcca taaacctttt cagcgacggt gttcagaact atcttgaccc
     1921 gtcatcaaga aagcttccgt cattcaagaa atacgaaaag aaacacggta taaagaagct
     1981 ttcagcagtt aaggcaaacg gggaggcgta aaagatgaaa aaaggaaacg ttcttgaaat
     2041 aaaaaacctt tctgtagact ttatgacagt tagaggtatc gtatacgccg ttcagggcgt
     2101 tgaccttgaa gtgaagcaag gggagataca tggtatcgtg ggcgagagcg gctgcggaaa
     2161 aagcgtaaca tcaaagtcgg tcataaggct ccataacgag gagaccacca gatatgcagg
     2221 agatatctac ataagagatg acagcggcga gaaagacgtt cttgcaatga gcaaaaagca
     2281 gctccgtgat ttcaggggca gcacagccgc aatgatattc caagacccta tgacttccct
     2341 tgaccctatc atgaaagcag gcgagcagat agcagaaatg ctaagggcga agaaaaactt
     2401 aaatcgccgt gaagcaagag aaaaggtcat cgagatgttt gaaaagatag gcataacacc
     2461 tgcggagaaa agatacgagc agtatccgtt tgaaatgagc ggaggaatgt tacagagaat
     2521 aatgatagca atggcgctta tatgcgagcc gaagcttctt attgcagacg aacctaccac
     2581 ggcgcttgat gttactatcc aggcgcagat actaaagctt ataaaaaagc tacagcagga
     2641 aagcggaacg tcggtaatat tcatcactca caaccttggc gttgtggctg agatatgcga
     2701 cagcgtaact gtaatgtatg caggaaaggc tgtcgagaca gcaagcgttg tggatatttt
     2761 tgaccaccct gctcacccat acacaaaggc gctccttgaa agcaatccaa gggagagcga
     2821 cacagaaaaa agaatgaaaa gcatacccgg aagtcctccg cttctgtatg aaaagttcaa
     2881 gggctgtgct tttgcaccga gatgtaagta tgctgacgat aaatgcagga gctgtgtgcc
     2941 tgttacagaa aatgtgtctg aggggcacag cacagcttgc ttcaaatggc gggaggtgac
     3001 atcacaatga gcgaagaaaa agacagacag ccttttataa gcgtcagaga cctgaaaaag
     3061 tatttctgcc ttgacaatta caagcttgca aaggcaaaga cgagatatat ccacgccgtt
     3121 gatgatgtta gctttgatat ctacaaaggc gagatattcg gcataatagg cgagagcggc
     3181 tcgggcaaat caaccatagg aagatgcgtt ctgaagctta tcgacgttga ttcaggcgag
     3241 atatattttg acggcaaaaa cataactcat gtgagccgaa aggaacagca ggaactgcgt
     3301 tcaagaatgc aaatggtctt tcaaaaccct cttgcttcct ttgacccgaa aaagactata
     3361 cgctttagcc ttgaagaatc tgcaaaggtg cataagataa agcctgaggt ctacaaggct
     3421 aggataaaac agcttatgga gtatatagcg cttccggagg acttgctcac aagatatcct
     3481 aaagaccttt cgggcggtca gctccaaagg cttgcaatag caagggcgct tatgctggag
     3541 cctgatttca ttcttgcaga cgagcctgtt tcggcacttg acgtttctgt gcaggcgcag
     3601 atattgaatc ttatgctcga cctgaaagag gataaagggc agacgatact ttttatctcc
     3661 catgatttga acgttgtccg tcacgtttgc gacaggatag cggtggtata tctgggagcg
     3721 gtaatggaaa tgggtacggt ggagcaggta tataacagcg ttgctcaccc ttatactcag
     3781 gcacttattt cagcaaagcc aaaggaacac cctcttgaaa ataagaagca tatcctgctg
     3841 gagggagata taccgaacgc agtggatatc cccgagggct gtcgctttgc aggcaggtgc
     3901 agatatttca aagagggctt gtgcgacaac cgcacgccaa agctcaaaga cattggaaac
     3961 gggcattacg ttgcctgtca tttcccgcta ggcattgacc gcagcaagac tgcttcggat
     4021 atgtaataaa tcttttgaag aaaggaaagt tatcatgaaa aaattctcac gcaagatcgc
     4081 ggccgctgct gcattggcaa taacagtttc aagctgtatg acaggctgct tcaacaacac
     4141 aggttccggt tcaggctcag gttcttcagg aggaaattca gagacagttc tcagagtagg
     4201 ctatactcag gagccgtcat catttgaccc tgccgacttc actatcgtgg cagcaactct
     4261 cacaggttat gactgctatg acacgcttct taacttctca catgacggca caaaagttga
     4321 acctgccctt gcagaatctt gggagcaggt tgacgatact acatacacat acaagataag
     4381 acagggagta aagttctcag acggcaatga aatgacaatg gacgatgtgc tttattctct
     4441 taacagagta acagaagaca actattatat gagctatctt tttgcaaacg ttgacagctt
     4501 tgaagccgac aacgatactt ggacgctgac agttcatctt aagcagcctg actcatcatg
     4561 gcagtatgtt cctgcaacat cagcctgcac tatagtcgag aagtctgtaa cagaggagca
     4621 aggcgacaag tacggcactg ctgacggaaa gactgtcggc acaggtcctt acaagctttc
     4681 ttcatggtca tcaggaagcg agatagttct tgaaaagaac gaaaactggt ggggcgaccc
     4741 tgatactctc gatatcgaca aggtagaata ttacgttatc gaggacgaaa gcgcattagc
     4801 acttgcggca aagggcggac agatagactt tgtcgagggt atgtcaaaca gcgttatgtc
     4861 tgtatatcag agctgtcaga acttcaatat cctctcatgc gagggaacga cttcaaacta
     4921 tatcgcattc aacacagctt caaagccgtt tgatgacgag tgggcaagaa aggctgttgc
     4981 atattgtatc gaccgttctc agataacaac aactatcggc ggcgattatg caaagcagtc
     5041 ggctgttgtt ccaatgagcg aatccatgat gtatatggat aaggataagt gggattcaac
     5101 tatcgccgaa agcgatgttt atactcagga ttatgacaag gtaaaggaat gtcttgcaaa
     5161 gtcaaagtat cctgacggct ttgagtttga tttctatacg acttcacaga cgcagaagca
     5221 ggctgagctt ctcaagtcaa tggtagacgc aagcggctct atcacaatga atcttgtgga
     5281 agtgcctgac agcgatatct tcagctatat gttcggctac aagacaaagg ataacggcga
     5341 gagatattac aacgcagtcg gcacatacat gatgtcagac ttccttgacc ctatgggaat
     5401 gttaaagaca ttatatgcag gctctaacgc ttgcgagggc ggaacgaatg aaacactttg
     5461 gacaagcaca gaagcagacg gacttcttga taaagcggct cagacaacag acgacgctga
     5521 gaaaatgggt tactatactg acgcatataa gatatttgct gatgagtgcc cttaccttgg
     5581 attttacaca tcatctgatg tttacgcagt aagcgacaag ttcacatatg acccaagccc
     5641 gatgttctgg tataacttca gttatgcaga cgttcatgtg gcaaaataag aaataatatg
     5701 aaaaaggaag tgcgataatg tattcatctc tggataccgt gtgggttctt ctctgcacag
     5761 ctttgatatt ctttatgcag gcaggctttg caatgctgga aacaggcttc accagagcaa
     5821 aaaatgcagg caacataata atgaaaaacc ttatggatta ctgcataggc tctgttcttt
     5881 tctgggttat cggcttcagc tttttgtatg gcgactccat aggaggattt atcggaacgc
     5941 caagcctgtt cgcagcagga aagtttgcag ccgcaggaga tctgcctaag cgagtgttcc
     6001 ttatgttcgc aactgtcttt tgttcaactg ccacgactat agtttcagga gcaatggcag
     6061 gcagaacaaa gttcaaggct tatcttacat attcggctgt tatgagcgga atagtttatc
     6121 ctataacagg tcactggata tggaacagtg caggctggct caaaagcata ggcttccacg
     6181 atttcgcagg cggaactgcc gttcacgttg ttggcggaac taccgcactt ataggtgcgc
     6241 tgcttgtggg agcaaggata ggcaagtttg ataaaaacgg caaagcgaga gctataccgg
     6301 gacataatct cactatcgga gctttgggca tattcatttt gtggataggc tggttcggat
     6361 ttaacggtgg ctcgcttctt accgctcagg gcgacaatgc ggcggcaaag cttggggata
     6421 tcttctttaa cacaaacata tctgcggcgg cttgcgcaat agctgcaatg ttcatcacat
     6481 gggtgcgcta cggcaagccg gacgtgacaa tgacccttaa cggagcgctt gcaggacttg
     6541 tggcttcaac cgcaggctgt gacacagttt cggcaggagg agcattcttt atcggcataa
     6601 cagcaggctt tgtaatggtg tttggtgttg agcttataac aaagctaaag gctgacgacc
     6661 ctgtgggtgc ggtaagtgtt cacggtctgt gtggagcatg gggaacgctt atgacgggcg
     6721 ttttcgcaaa gaaaagcggt cttatatatt caggaagccc gaaggatctg cttatacagc
     6781 ttgcaggcgt gctttcagtg gcggcatgga cagcgcttat tatggtgata gtgttcaccg
     6841 tgataaagaa aacaatgggt ctgagagtta gcgagaacac agagatagtt ggtcttgaca
     6901 agtgcgagca tggactttca agcagctatg cggatcttct caacactgct cagttcatca
     6961 ccgctgccgc tgacgagcct tcgaaggtga gggctataga cgaagcctca gagcttaatg
     7021 cttctgacta caaggcggac ggaaagatgc acaaggtggt cgttcttatg aacgtcgcaa
     7081 agttcgagat gctgaaaaat gctttggata agcttgatat aacaggtatg acagttacgc
     7141 aggtgagcga ctgtggaata caaaaaggaa gcactgagct ttacagaggc tctgagcttg
     7201 aatcaaggct tctgcctaag ataaaggtgg agatagttat aagcactgtt cctcttggac
     7261 ttcttatcga cactatcaga aaggtgcttt atacgggcaa gataggcgac ggaaagatat
     7321 ttgtttatga cgttgcaaac gttgtgaaga taagaacagg tgcagaggac gagcaggctc
     7381 ttgaataagc tgtcataata attggaatgg gggacaagat atggctgatg atatgaaata
     7441 cactgtgttt tatacagacg aaaacggaca gcttgtcccg aatggggaaa agcttctgag
     7501 cataaccgag atagcaaagt atcgaaacat acctaccgaa cggcttagat attacgataa
     7561 gatagggctt ttaagacctg actatgttga caagctcaca gggcggaggt tttattcggc
     7621 acagcagtgt gaaaagcttg gcactataaa agagcttcgc aggctgaact tatcactgaa
     7681 agaaatagag gactatttca gcgacagaaa ccttgaaaag tccgagcgtg ttctcagaca
     7741 gcgttatgag cttttacagc aggagatagc ggataagctt gaaataagcc gtgtgctgaa
     7801 agaaaagctt aaatttatca gagagcttaa caacacagat ttcaagcttg atgaccctgt
     7861 tataaaggag cttcctgaac gatatgccct caaaggggag agcaataagt tcagaacaaa
     7921 tctcactggc atagagttta tgaagcttga ggaggctata ggcggaactt cgcctgtgct
     7981 tgccactgat aaggtggcaa tgaccataaa aaactcagtt gaccctgacg atatggattc
     8041 cgaggtctgc cctatggtgt tctgctcggc agatcaggct gacaaggaaa gcttcacaac
     8101 agtggcagga ggaaaatacg tttgcgcttt tcacaggaat tgctacagct atttcggtga
     8161 gcttataatg aaaatgcgct gtcttgcaca ggagcatggc tacacccttg gaaacgtcgg
     8221 ggtgatgata taccgtatag acataacact tacggacaac agtgatgaaa ctattgcgga
     8281 gcttcagttt ccgcttataa aagaaagcag ggagaaataa tcatgataga cagaaatgaa
     8341 ctgcacgcca tgaaaacggc ggcaagattt gatgaatttg gcaacacaag atttcctaca
     8401 atggcaaaga gagccgttga gcttggatta gagcctgcgg acagcattaa aagcgagggc
     8461 atgagctgtt ttgcaagaac aaatctcggc cctacataca caagccacga gacttttctc
     8521 agaagccctt tctgcgagga tatccataag gtgtcggaat atgacgttgc agtggtgggt
     8581 atcccatatg acagcggagc gtcaaaccgc tcaggcacaa gacttggccc tgccggcata
     8641 agacacgctt cttcgcagat atcccctttc aaccctgact atggcgttga cctatacgaa
     8701 aacataaagc tttgcgacgc aggagatatc tacacacttc ctgcaaatgc cgaaaagact
     8761 ttcgaccaga taacaaaggg cataggatat atattctctc agggagtaac gcctatcgtt
     8821 cttgggggcg accactctac cacttttcct acgctcaggg gcatttcaaa gtatatcgac
     8881 ggcaatatcg gcgttatcca tatcgaccgc cacagcgatt gcgacgctat ccaaatggac
     8941 gaaaaaatgc acggcacaga atggtatcat tccactcacc tgcctaacct cagcgcaaaa
     9001 aatcttgtgc agataggcat aggcggaaac tgctgcaagt catggtcaaa attcacccgt
     9061 gacagcggct gtactgcgat ctcaatggag gatatagaca agtatggtat cgacaaggtg
     9121 gcagagcttg ccctggatat cgcatggaag ggctgtaagg cagtgtatgt gagccttgat
     9181 atagacgttc tggaagctgc tttctgccca ggtactggca cacctgattt tggcggtatg
     9241 ctcccgagag agcttttaag gcttcttaag ctcgtgacag ctcccggtat ctgtggaatg
     9301 gacgttgtgg aggtatctcc tccgtttgat gtgagcgaga taacttcact tgcggcgtca
     9361 agatgtataa tcaatgtgct ttgcaatctt gcgctgaaca agtcgctata taaacactga
     9421 taatatttgt atctgaaaga ggcagcttat gaatgatcgg cagctaaagg aacttatact
     9481 ggcggctaag ggtaagctgc cttttgatat ttatttttac aacgcaagga tagttgacgt
     9541 ttatagagaa cggataatag acagcggctc ggtggggata aaaaacggca ttatcgccgc
     9601 agtttgccct gactttgagc ctagagcaac agagtttatt gattgcagag gaatgtattt
     9661 tgcgccttct tttatcgacg ctcatatgca cgtagagtcc tcaaagctca cgccttttgc
     9721 atactgcgag ggcgcagtgc cacacggaac gggctgtgta ttctttgacc ctatgcagat
     9781 gtccaatact cttggtctta agggcataaa agctataagc gaaatgcttg gggaaatgcc
     9841 catagagagc tttttgcagc tttcttcgca gtatctttcc accctgaaaa cgcaagacga
     9901 tatcaccaat gttatcaaag agtgcagggc gaaaactgtg ggtgagataa gcggtgctgt
     9961 ggtggggaat gaggagataa taaagcttct tggtgcggca aaaagggcag gtgtaaaggt
    10021 gaacggtcac tgtccgtcaa tgggatttcg tgacgttcag caggcggctt gcgccgggct
    10081 ttgtgacgac catgagtgcg aaagcataag cgaccttgag cagcggctgg cctgcggaat
    10141 ggcggtgttg gtaagagagg gaactataga gccgaactgc cgaactcttt gcgaggggat
    10201 agtaaaaaat catatcccta cggacgatat aatgttctgc actgatgaca agtctgcgga
    10261 ggatataaag gaaaacggct gtatagataa tgctctgcgc attgcagtga aatgcggtat
    10321 ttcccctgtg tcagcgataa aaatggcaac tctcaacgtc gcaaggcact atggtgttga
    10381 cgatatcata ggctcggtgg ctccctcaag aaaggcgaat tttctgctta tggatagcct
    10441 tgaaaatatg acagtgaaaa aggtctttca ccagggcagg cttgtggctg aaaacggcga
    10501 gtttctttgc aggcatgagt ttgatataac ttgctatcct gagcttaaaa acactgtgat
    10561 attgcccaga gggcgtgaca gacattcttt tgagataaaa gcaggctttg agaggggtta
    10621 tgttactgca aatgcaattg aaatgcccga gggtggcata gtcacaaggc ttgtaaaagg
    10681 caggcttgag gtcagcaacg gcattgtatg cggtgataca aaagccgata ttctgcccat
    10741 attcgttgtg gagcgtttcg gcaggagtgg tgagatagga agtggcttta taaaaggtct
    10801 tggcattaaa aggggagctg ttgccgcaag ctttgttcag gagagcaata atgtcgttgt
    10861 atcgggcgtt gaccccgcgg atatgctttg cgctgtgaaa gctgttgaag ccaacggcgg
    10921 cggtgcggct atggctattg acggcaaggt ggcagggctt tactcccttc cagtggcagg
    10981 catactaagc ggtgaaaaca tcacaggcga gataaaggca cagaaagctt tcagagaagc
    11041 ccttgccctc acagggggaa acatacagcg tatgacggtg gtgcttacag tgtcgctgtg
    11101 tcagactatc ccagaggtgg ggctgacgga aaaagggctt tttgacgtaa accagcaaag
    11161 atacataccc acattcacag gcggtgaaga atttgaagaa taagccagaa aatgtaataa
    11221 tgacagaggg cgaccctaag aaaataatat tccgatttgc acagcctctt atatttgcaa
    11281 acctttttca gcagctttac aacaccatgg acaccataat agtgggcaag ataaatggtg
    11341 acgaagctct tgcggcggtg ggagtttcct ttgcagtgac aatggtcatg atagccgttg
    11401 caactggtac gggcatagga acatcggtgc ttatagcgaa atactgcggt gccgggaaca
    11461 agtcaaaggt caagacgggc atttccactg tgctgatatt ttcacttatg gtcgcagcgt
    11521 tgatagcggt tcttggggtg gtgcttagta tgccgctttt aagacttctg aaaacgccgc
    11581 agaatatcat aaatgacgct gcaaactatc tgcggatata ttccttaggt atgccgttta
    11641 tgtttcttta caacgtgcaa gcgtcggtat ttagtgccct tggcaactca aagacgcctt
    11701 ttcatctgct tgtgatgtcc tctttgctga acatagggct tgaccttatg tttgtagggg
    11761 gattttccat gggcgtatca ggtgcggcat gggcgacttt cattgctcag ggagtatcgg
    11821 cagtgatatc gtttctgctt cttactgaca aggtatacag aaaagaccat gaaagggcag
    11881 agttcagctt cttttcgggg gcggtgttaa aagagatgtc gtcctatgcg gcagtatcgg
    11941 tgatacagca gtcagtggtg tcggtgggaa tgttaatagt gcagtctgcc gtcaatccct
    12001 ttggctcgga cgtgcttgca gggtacacgg cggcttcaaa gatagactcc tttgcgataa
    12061 tgccgttcat agcttgcgga aatgcggtat ccacctacac agcacaaaat cttggggcag
    12121 gtaaaaaaga gcgtatcaga gagggctaca aggcgagcgt gctgttatgt gcaggcatag
    12181 cggtgataga atttgcagtg ataatgcttc tcaggcggcg attcttgggc ttttttatgg
    12241 acttgtcaag ctcctcagct tccgccatag aaacaggcat gggctacatg acagaccttg
    12301 cgttttgtta tataataatg ggaataaatt caagctttaa cggaatgtta agggggcttg
    12361 ggtgtttgaa gctgttttta tccggaagca taataaatct cacgttccga attattttca
    12421 cctacgctct ggtatcggtc ataggcgtat cggcagtatg gctgtcgatg ccggcaggct
    12481 gggttctgag ttttatttac gggagagtgg ggtataggat catgtgcaag aagaaagata
    12541 gtgcagggat aagcgatata tgataggtaa aagcattagc ttccgagcag ttgtcagtgg
    12601 ctgttcggaa gctgttttta ttgtaaaaca aaaaactcgc aaaccctcgt agataaagga
    12661 tttgcaagct ctttaacttg tcttgctgat accaaatgga tgcatatcta aaacgagata
    12721 aattatgacc attccgatta aaaattattt ctaccggtca atgttcattg aactcatcga
    12781 tcattctgcg atattctttt tcctcacaat ccacataatc cttagcaaac tgctccgctt
    12841 cttcaagact gtctttcaac agttcaaaat tcaccgcagc cgaaaaaaca tcaatttcaa
    12901 gcttttcgga c
//
LOCUS       DAWEMR010000067        12847 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2049_length_12847_cov_12.025954,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000067 DAWEMR010000000
VERSION     DAWEMR010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12847)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12847)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12847
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_12847_cov_12.025954"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3662..3671
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3803 a         2636 c         2765 g         3633 t
ORIGIN      
        1 ataccgacaa ctgtatctct aacgatctgt ggtatgtcga ttatagcctt tgttgttatc
       61 tcgcccatac acattactac acctgttgta gtgaccgttt cgcaggcaac tcttgacata
      121 ggatcttgct ctagcagagc gtcaagtata gcgtcagaga tctgatcgca tattttgtca
      181 ggatgtccct cagtaaccga ctcagatgta aataaatact ttgacataaa aatacctctt
      241 ttctgaatat ttgttaatct aaaaacagcc acaaaaaaag cgcttcattc cgaaacgctt
      301 gaaattactc aatttcctca tctttcggaa ataaccgcag gatttagcac cttaactaac
      361 gctaggttgc tgggcttcac aggacctgtt tcctccacca ctcttgataa ggctatttaa
      421 ttttaacaat atatattata caccatatat cgactattgt caactgtttt acacataatt
      481 atagattatt ttaatgtttc gtaataaatc agcaaatgct aataaaagca ttgacttact
      541 atgacaagtt cgttataata taaaaagagg tgagtttttt ttgaaaaatg acaattcaaa
      601 tataagaaca ttgtttctga cctttcttaa gataggcgtt tttacctttg gtggaggata
      661 tgcaatgata gccttgctcg aaacagaatt ttgtgtgcgc aaaaattggc ttgataaaaa
      721 agaatttctc gatatgacag ctatatcaga gtccaccccc ggaccaactg ctgtaaacag
      781 tgctacatat atcggctaca aaataggcgg tgtgctgggt gctattgcgg caactgtcgg
      841 tgtttgcata ccgtcgtttt ttataatcta cataatatca cttttctttg acaaatttct
      901 gtctttaaaa gtagtctacg ctggattccg tggcattcag gtgggcgtaa tatatctcat
      961 acttactgca ggactaagaa tgttgaaagc acttgataac acattgcttg acaaaattat
     1021 aatgcttgga gttttcataa tgatgatact tttcacactt ttttctgtga agttttcatc
     1081 agtattttat attatcataa gtggtgctgt gggcattgcc gtgtatctga ttaaaaccct
     1141 cagaaaggga agtacaataa aatgatatat ctcaaacttt ttctcatttt tcttgaaata
     1201 ggcgcagtgt cattcggcgg aggatatgga atgatatcca ttatccgtga aaaagttctt
     1261 acctttggct ggctcagtga ggacgaattt gtgaacttta ttgccgtatc tgagtccacc
     1321 cctggaccac ttgcagtgaa catggcgacc tttgtaggct catcgcaggc aggcttcata
     1381 ggcggattga tagccactct tggcgttgtg ttgccttcgt tcgtgataat attcatcata
     1441 gcttcgatca tgaaagattt gctgaaatac tcaggggtca aagcaatgct ttcaggcatt
     1501 cgtccttgcg ttgttgcaat gatactcgcc actggcatta caatggcttt gtcttcactg
     1561 ctaaatatca ccaccataaa ttcaaagcct gcttttgact ataaagcgtt gataatattc
     1621 ctgctcatag ccttggcaga tgtgcttctt ttgaaactga agaaattcaa gctctcaccg
     1681 atcacaatta tcgtaagctc cgcctttctc ggcataatat tctattaaaa aaagctgtaa
     1741 catcgcaaag tgctacagct tttaatattt gtgatatgaa aattaaccca gaagcttttt
     1801 aagtctctcg tcaacagcct taaggaagtc cactgtattt actttcttct tattttcaag
     1861 tgtagaaagc agataaagat cacctgtcat aacaccgtct tcaatagttt cgatagttgc
     1921 cttttcaaga caatcagcaa agttcataag ttcaggcagg ttatcgagtt cgcctctttt
     1981 tctcagagcg cctgtccatg cgaacagtgt ggcaactgag tttgtagaag tttcttcgcc
     2041 cttgaggtgc ttgtaatagt gtctttgaac agtaccgtga gcagcctcat attcatagat
     2101 tccgtcaggt gaaacaagaa cagaagtcat catagccagt gagccatatg ctgtagctac
     2161 catgtcagac attacgtcac cgtcatagtt cttgcaggcc cagatataac cgccgtttga
     2221 acgaataact cttgcaacag catcgtcgat aagagtatag aaatactcga taccggctgc
     2281 ctcaaactta gccttgtact cgttgtcata tatatcctgg aagatatctt tgaatgtgtg
     2341 gtcatacttc tttgaaatag tatcctttgt agcaaaccat acgtcctgct ttttgtcaag
     2401 agcaaagtta aagcaagctc ttgcgaatga agagatagaa gttgacttgt tgtgctgacc
     2461 ctgaatgatt ccgtcgcact caaagttgta aattgtctct cttgtttcat tgccgtcctt
     2521 gtcagtgcaa acaagctctg cctttgaacc agcagggcat ttcatttcaa cgttcttata
     2581 aacgtcgccg tatgcgtgac gagcgatagt gataggcttt gtccatgtag gaatgtatgg
     2641 agtgataccc ttaacaagga taggagttct gaaaacagtt ccgtcaagga tagctctgat
     2701 agtgccatta ggggatttcc acatttcctt gaggttgtat tcaggcattc ttgcagcgtt
     2761 aggtgtgata gttgcacact taacagcaac gccgtatttc tttgtagcgt ttgcagaatc
     2821 aacagttacc tgatcgttag tttcatttct gtgaacaaga ccaaggtcat agtattctgt
     2881 tttaaggtca atatatggag taagaagaat atcctttata tcctgccaga taattctggt
     2941 catttcgtcg ccgtccattt caacgagcgg tgttttcatc tgaatttttg ccattggtaa
     3001 gacctcctat aaagtaataa tggaatatcc gttgttttta gctatcgcag atatcttttt
     3061 gtaaacatca agacttgcaa tgataagctc atagctgtcg ctgtcaatat ctatcataca
     3121 tatgcaagca cctttgagag tgctgtcctt tgtgccgaaa agtttcgaga agatccccat
     3181 aggaaccttc atatcagact ttattattta gagagctgtt ctcttgtgta gtcaagcaga
     3241 ccacctgcaa ggataatatc ctttgttctg cctgtaagct cgcagagaac tggtatctca
     3301 acacctgttg tcttattcac aacagtaagc ttagacatat cagcctcaac gtctgcacga
     3361 acgtcagcaa gaacgatctc atcgccctga tttatcttgt catagtcagc ttcatttaca
     3421 aatgtgagcg gaaggatacc tgcgttgata agattagctc tgtgtattct tgcaaagctc
     3481 ttaacaagaa cagcctttat gccaagatag agaggagcaa gtgctgcgtg ttctcttgat
     3541 gaaccctgac cgtagtttga gccgccaacg attattgact tgcccatgtt ctttgcacgt
     3601 cttgggaagt cctcatcaca tactgtaaag cagtgctgtg agatagcagg gatatttgaa
     3661 cnnnnnnnnn ngtggaagta tctttgcgcc tgccggcatg atgtggtctg ttgtgatatt
     3721 gtcacccacc ttaagtgata cctgacaatc aatgctgtca tcaagcggtg aagtctgtgg
     3781 gaaaggcttg atatttggtc ctctaagaac ctcaacgctg tccatatcag cctcgtcagc
     3841 tggcggaaca accatattgt cgtttatctt gaaatgttca ggaagcttga actcaggcat
     3901 atctccaagt gttcttgggt ctgtgaggta gcctgtgaga gctgaaacag cagccatttc
     3961 aggtgatacc agatagatct gaccatcctt tgtgcctgaa cgtccctcga agtttctgtt
     4021 gaatgttcta agtgaaacac cctttgagtt aggtgactga cccataccga tacatgggcc
     4081 gcaagcggat tcaagtattc ttgcgcctgc tgctatcata tcagcaagtg cgccgttctc
     4141 agcgatcatg ttaagtacct gttttgagcc tggagcgatt gcaagtgaaa cgctagggtc
     4201 aactgtcttg cccttaagaa tgtgagctac tttcatcatg tcaacataag aagagtttgt
     4261 gcatgagccg atacaaacct gatctatctt aagctttccg atctcattta cgctcttaac
     4321 gttgtcagga gaatgaggac aggcagccag tggtacgagc tgtgaaagat cgatgtctat
     4381 ctgttcgtca tagacagcgt catcatcagc ctttagctcg ctccatacgt cagctctgtc
     4441 ctgagccttc agaaatgcaa gtgttgtttc gtcagaaggg aagatagatg tggttgcacc
     4501 aagctcagca cccatatttg tgatagttgc tctctcaggg actgtaagtg tctttacgcc
     4561 ctcgccgcag tattctataa ctttgcctac gccgcccttt acagacattc ttctgagaac
     4621 ttcaaggata acgtccttag ctgataccca tggagaaagc ttgcctgtga gatttacctt
     4681 tactatctta ggatatgtga tgtaataagc accaccaccc attgcaactg caacatcaag
     4741 tccacctgca cctatagcga tcattccgat accaccgcct gttggagtgt ggctgtcaga
     4801 gcctataagg gtcttacctg gtatgccgaa acgctcaaga tgtacctgat gacagatacc
     4861 gtttccggga cgtgaaaaat aaatgccgtg cttcttgcag acagaaccga taaatctgtg
     4921 gtcatcggca ttctcaaagc ctgactgaag cgtgttgtgg tcgatataag ctacagactt
     4981 ttccgttctg acacgaggaa cgcccattgc ctcatattca agataagcca ttgtacctgt
     5041 tgcgtcctga gtaagtgtct ggtcgatacg gataccgatc tcctgaccct taacaaattc
     5101 gccgtcaaca agatgtgctt taagtatttt ttctgttaaa gtaagtccca ttgtaacaac
     5161 aatcctttca aaaataactg atattaatat tttataacaa attgcgattt ttgtcaaggt
     5221 aaatataaac atttgcttga ttttatagcg ttttagtgca tatttttgca tggtatacgt
     5281 caatttactg tataaatttt catttcttac gatatcctta cttgacacgg caaagcgtat
     5341 attatataat agttgtgtta tcagagaaaa caaggcggtg aaaagcatga aagcaaaaac
     5401 tgttttgata ttattatcgg cggtgatact atgtctgaca ggctgtacag agtcacaaaa
     5461 gcccacaaca attgtggata attcctctgc cgtgactaca tcatcaagtg ctgacagcga
     5521 aagcggcaat gataaatact atgacagcac gcctatatct caggcttatc tctcaggcaa
     5581 tacagcaaat cttgatgata aacagcttga aatatacaat aaagccgttt cagccattga
     5641 aaaacttatt acccttgaca tgaacgatta cgaaaaagag cttgcggtac atgattatat
     5701 agtcggctgt gcaagctatg acgaagcaca tctcaatgcg cttggtattg gtctgccaaa
     5761 ctctgataat ccatatggct gtcttataaa cggaaaagca atatgctcag gctattccac
     5821 aacgtttcag ctttttatgg atatgttgga aataccctgc aagaccatat acgccacaga
     5881 ttttgagggc gacgatcatt catggaacat agttcagata ggcggtagtt ggtattatgt
     5941 tgacgtctgc tgggacgacc ctatccctga ctttacggat agaccagtaa ggcacaagta
     6001 ttttaacgtt tcaaaagaat acatgaaaac aaaacacgtc tgggacgata cagagcttcc
     6061 tgatacagat agtacaaagt tcagctatat tacgcagaca gttacgcata taaactcagt
     6121 aaatgagata gagagtctta tggaaaaggc tttggataat atgcaggaca gcgtggcggt
     6181 aacgtcagat atgagcgatt ttcaattcag tgaaactgac cagatagaca cgaacttcaa
     6241 tgaggaaaat atcaaagggc ttagcaaagt ttttgacagc ttctgcgaaa agcatacaga
     6301 tcatcaagta tactgtcaac gcgttcaaaa cggaaatgat atagttttgg tgatgtacat
     6361 gagaaaattc agataggaga gagcaatgaa attcagacct tgcatagata tccataacgg
     6421 taatgtaaaa cagatagtgg gcggaagcct ttctgacaaa aataattatg ctgtggataa
     6481 cttcgtttca gaatatgacg gagcttatta tggcaagctt tataagtcat acggactgac
     6541 ggggggacat attattatac tgaaccctgt cggcagcgaa ttttacaacg cagacctcga
     6601 acaagcctac agtgcattat ccgcatttga gggcggattg cagataggcg gcggaataaa
     6661 tgatgaaaac gccgcacaat ttcttgaaaa gggcgcaagt cacgttatcg tgacgagctt
     6721 tgttttcaaa aacggtgttg ttaatttcga taatctgaac agactggtga agtccgttgg
     6781 caaaagcaga cttgttcttg acctttcctg ccgcaaaagg gacgggaagt attacgttgt
     6841 gaccgaccgt tggcagaaat tcaccgatat tgaaatgaac agccagacct tagaaatgtt
     6901 ctcaaaatat tgtgatgaat ttcttatcca tgctgttgac acagagggca aagcaaaggg
     6961 cattgagccg cagatagcaa agataatggg agaatactgc accattccat cgacctatgc
     7021 cggaggaatt tcatcatttg aggatctcga taacctgaaa gaactcggca ggggaaagat
     7081 agatttcacc ataggctctg cccttgacat tttcggtgga aatatgaact ttgaaaaagt
     7141 cgctgtctac ggcaaatagc cacgccttat gcaataaaca caatatttat agctgtttgc
     7201 tgcatatttt ggcacaaaaa gatttgcaat tttagcggaa atatgttata atattatgat
     7261 actaattcag aaaggacgaa tttttatgtc taagatccga gttgccgtta ttttcggcgg
     7321 aaaatcaaac gagcacgacg tatctgttgt ttctgcaact cacgttataa agagtatacc
     7381 aaaggacaaa tatgatgtta tttgtatcgg aataacaaaa aaaggtcact ggatgaaata
     7441 tataggagat gttgacgata ttgcgtcaaa caaatgggaa cagcaccctg ataatgttcc
     7501 atgcgttctc agccctgacc ctcttcacaa gggctttatc gctcttggcg aaaacggcga
     7561 atatcagaat gtaaaagttg acgttatttt cccagtcctt cacggaaaga acggtgaaga
     7621 cggcactatt caggggcttt ttcagcttgc tgagatacca tttgtaggct gtgactgcat
     7681 atcctctgca aactgcatgg ataagggact tacacacact gttcttgacg caaacggcat
     7741 aaagacagca aagtgggtag gcattataac ctcagagctt tcacatcttg acaaaaagtg
     7801 cgacgaaatg gaaagcaagc ttggctatcc tatgttcgtc aagcctgcaa actgtggttc
     7861 ttcggtgggc gtttcaaaag ctaaaaacag agacgagctt aaatctgcta tcaagcttgc
     7921 atttgctcat gacagcaagg tcattgtaga agaaactata gtaggcatgg agtgcgagtg
     7981 tgcggtaatg ggcaatgacg agccttttgc ttcaactgtc ggtgaagtat gctccgcaaa
     8041 tgacgagttt tacgattttg acgcaaaata taatgacgcc gcttccaaaa cgcttatccc
     8101 tgcaagaatg agcgaggaaa gcattgaaga aataagaaag acggctgtca aggcatacag
     8161 agcaatgggc tgcagcggac tttcaagagt agacttcttc cttatgaaag acggcactgt
     8221 tattcttaac gagataaata ctctcccagg tcacacgcct ataagtatgt atccacagct
     8281 tatgcagtac gagggaatga cccctgcaca gcaggaagat aagcttatca agctggcact
     8341 tgataaggcg gaggttgatt atgaataacg ccgccattgg agttttcgac tcaggagttg
     8401 gcgggcttac ctgtgtgaaa gagcttataa agcttctgcc aaatgaaaat atcgtttatc
     8461 ttggcgacac ggcaagagtg ccatacggct caaaaagcag agagactata atgagctatg
     8521 ccagacagga tatagagttt cttaaacagc ataacgttaa aatgatactc gttgcttgcg
     8581 gtacagtttc ttctgtattg cctgcaatgg atataaagtc agatatctcc ataagcggag
     8641 ttgttgagcc tgctgtaaag gctgcctgct ctgctacaaa gaataacagg ataggcgtta
     8701 tagccacaag tgcgaccata aagagcggca tttacggcaa agctataaga agcattaacc
     8761 ctaacatcaa ggtggtcggc aaggcttgtc ctatgttcgt gccactggta gagaatggct
     8821 atactgcccc tgattgtgtg cctgcaaggt atatcgcaca ggagtatctt gaaataatga
     8881 aaaaagaaga cgttgatacg cttattctcg gctgtactca ctatccacac ctcactgaac
     8941 taatttcaga tataatgggc gagggcgtaa cacttgtttc atcaggtgcg gagcttgcga
     9001 aatatgcact caactccctt tcatacagcg atgatacggc tgaaagagaa gagcgtgaga
     9061 gccttgagct ttactgtacc gacagcgttg agctgttttc tgagaatgtt gaacgctttc
     9121 tcggaaacaa tatcaatgca caaatatcaa aatgcagcct aaagctgtaa actggaggat
     9181 tttatttgac cagcaaggaa gtatcaataa aacttgttag ccgtcaaagc gacggtaata
     9241 caaaagaaga aacagagctt atctcagcag gactttatga agcaaccgaa aaaggatatc
     9301 gcatatcgta tgaggagtct gaggcaacag gatttgaagg aagcgttaca acgctagaaa
     9361 ccgaaggctg ccgcaaggtg actatgaaca gaaccggaac ggttgaagcg aatcttgttg
     9421 ttgatatagg cgaaaagcag cattgcgttt atggcactcc atacggtgat tttatgatag
     9481 gcataaccgc taaaaccata agatcagatc ttgacgaaaa cggtggaatg cttttctttc
     9541 actatgtttt agatgtcaat tcaagctata tcggtgattt tgagatagct atagaagtaa
     9601 aagcggcaaa tgttgtcgca taattatatt tggaaggacg gaaaaataat atgactaatc
     9661 tcataagcga cgcatttatt caggctaagg agcttgtaat gaaggctatg ggcgagcttg
     9721 ttgcagacgg aacctttcca gcagagcctg ttcctgcatt taatacggag atacctgccg
     9781 actcaaaaaa cggtgacgtt tccactaatg ccgcaatggt atgtgcaaga cctttcagaa
     9841 acaatccaag aaaaatagca gaagctatcg tatcaaagat agaccttaac ggctcatact
     9901 tcgcaagatg tgaagtggca ggtcctgggt tcataaactt tttctattct actgaatggt
     9961 atgcaactgt agttgccact gttttggagc agaaagagaa atacggtgaa actgaccttg
    10021 gcgcagggaa gagcgttctt gtggaatttg tttctgcaaa tccaacaggt cctatgcaca
    10081 taggcaacgc aagaggcgga gctataggcg attgtcttgc gtctgttctt gaaaaggcag
    10141 gctatgaggt agcaagagag ttctatatca atgacgcagg caatcagata gagaagttca
    10201 agacttcact cgaagttaga tatcttcaga tatacaagcc tgaaactgaa cttcctgagg
    10261 acgcttatca gggtcaggac ataattgacc atgcaaacgc attcaatgag atatacggcg
    10321 acaagtatgt gaacgctgac agtgaagaac gccgtcaggc actttgcgat tttgctctgc
    10381 caaagaacat tcagaagctt cacgatgatc ttggcaaata tagaatacag tatgacaagt
    10441 ggttcaacga gagcacactt cataaggacg gctcagttca gcgagttatc gaacagctca
    10501 aagcaagcgg tcatacctat gaaaaagacg gtgccctttg gtttaaaaca acagagttcg
    10561 gtgacgaaaa ggatagagtt ctcgttcgtg aaaacggtgt gccaacatat cttgtacctg
    10621 atatagcata tcactacaac aagcttgcag taagaaagtt cgataaggct gtagatattt
    10681 tcggtgcgga ccaccacgga tatatagctc gtattaaagc ttctatgaca gcacttggcg
    10741 ttgacgctga cagacttgac attgttatca tgcaaatggt aaacctcgtc cgtaatggtg
    10801 aaaagtataa gctttccaag cgttcaggca aagctatcac tctgtcaact cttcttgacg
    10861 aaataccgat agacgcagca agatttttct tcaatctccg tgagcctaat tctcattttg
    10921 attttgacct tgaccttgct gtatctcaga caagccagaa tccagtttac tatgtacagt
    10981 atgcacacgc aagaatttgc tctgtcctta aaaagatgaa cgaagagggc attgaagtta
    11041 agagcctcga caaggcggct ctcagcgtcc tcacagcgcc tgaggagcag gagatgataa
    11101 aacaccttgc tactctgcca aacgttataa acgaggctgc aaaggcttat gacccagcta
    11161 aggtaacaaa gtatgttata gaccttgcta caatgtatca caagttctat aacaactgcc
    11221 gtatcatggg agaagacgag agcgttatgc aggcaagact ttcactttca cttgcagtta
    11281 agcaggtaat aaagaatatt cttgatatgc tcaagataac ttgcccagag tctatgtaat
    11341 aaaatatcag acccgaccgt ggaaaactcc acggtcgggt cttttgttat ttctgggtat
    11401 ttcttatgta gtggaataga tattgctgtg caatgccctc aacacccttc acacaagagg
    11461 gaagtccgtt cggataccac tgtgcaagca ctcgcttcac ccacacgtct tttgggaaag
    11521 cgtccgtttt gtaaaagccg aaaagcagtg tacaatcagc aactttcgga ccaacgccct
    11581 tgatcttcat aagctcgttt cgtgcatcgt caaggggcat ttcttttatc ttttggtagt
    11641 ctatctcacc gctcaaaacc ttttcaatgg cgtcaacaag atattttgct ctgaatcctg
    11701 aacgcagata cgcaaggctg tctatagttt catctgccat atcttcagca gaagggtagc
    11761 agctataatg ctcacacaac cgtcctataa ttccctttat ccttgggata ttgttattct
    11821 gacttattat aaaggaagat aatgcctccc aactgtcctg cctgagtatt cttatgccgc
    11881 ctgcaaatga gcaggctttt gacagagttt cgttctcaga gaagcggcgt ttaagctccc
    11941 cataatcagt attcaggtca aaataatcga tccaaatgtt ttcaaagttc tgcacagtag
    12001 tgtcatgaag cctaaacacg ccattttcgg gttcctcgct tatggtaagt ttgttgttaa
    12061 ggaaagctcc gctgtaagtt tttttatcta tcttctccca gcgaaaagcc tgtccgcaat
    12121 caagtgtgtc atctagtcca aaatcaggct gtttcagcaa aatgtcactg ccgctttgtg
    12181 tataatccat aaaattcagc tcctatttat tataaaatca tcgaatctac ttgcaaaatt
    12241 ctacaaaaaa tattatacta taaatatgag tatttttcaa gcggataaaa taaacatatc
    12301 taagaaagga tagatcatat gagagaaagc attctgacaa tacctgtcac agatatattt
    12361 gagccgaaat gcggctgtcc aatatgccgc ctgcgtgata cgttggagca gagaactgtt
    12421 gagtacataa tgggagcggc tatgatggag cctgacgtaa ggcaggatac caacaggctc
    12481 ggtttctgct ttacacacta tcagcagctt atgatgcaga acaaccgcct gtcgctcggt
    12541 cttatgctca acagtcatct tgaggagctg aggggcaata tttttgagaa gaagggttta
    12601 tttgcgccca aggacgcaaa ggctaaaaag gcaggtgcaa taggcgatac ctgctttgtc
    12661 tgctcaaagg tgcagtgggg cattgaccat atgctcgaaa cggtattcac aatgttcgca
    12721 aaggacggaa aattcaagaa tctgttcaag tcgcaggagt gcttctgtat gcctcactac
    12781 cactatcttg tacagatgag cgaagcaaag atggcaaaga acgacagagc cgagttcata
    12841 aaggcta
//
LOCUS       DAWEMR010000068        12504 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2129_length_12504_cov_11.033738,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000068 DAWEMR010000000
VERSION     DAWEMR010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12504
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2129_length_12504_cov_11.033738"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3713 a         2518 c         3157 g         3116 t
ORIGIN      
        1 cttgtgtttc ttattatcgc agcggtacgc cgtaagcctg tcaaccctga gcatgaggga
       61 atggtacacg ctatcggtat ggcggctctg ctcctgctta tggtagtggt gacagtgagc
      121 gatatcatca agcttgttta aaggaggaac tgtttatgtc aggtgaaaat aagagaaacg
      181 taagacctgt caaggtgggg ggacttactc ttgacggcaa aaagatatat atccaatcca
      241 tgctcaatgt gccgtctacg gacattgaag gctctgttaa gcaggctgtg gagcttgaaa
      301 aagctggctg tgaaatagta agagcggcta taccaaacat ggaagccgtt aagcttatcc
      361 ctgctatcaa agagaagatc tctatccctc ttgtggcgga tatccacttt gactatcgcc
      421 ttgccattgc ggcggtggaa gctggtatcg acaagataag gataaatccc ggaaatatcg
      481 gctctatgga tagggtgaaa gcggtggtta aagcctgtcg ggaaagaaat atccctatca
      541 ggataggcgt gaacggcggt tctctggaaa aggagcttct tgcaaaatac ggtgcaccaa
      601 ctgctgaggc cctagtggaa agcgcaatgg gtcatgtgag aattttggag cagtgcgact
      661 ttgaggatat cgttatctct ataaagtcat cagacgtgcc gactatgata aaggcataca
      721 gattgtgtgc acagacctgt aactaccctc tgcacttggg cgtcactgag gcaggtaccg
      781 agagaatggg tcttataaaa tcatctatcg gtataggttc gcttctgtgc gacggcatag
      841 gcgaaactat aagagtttca cttaccgacg agccagtaaa agagatatac gccgcaaagg
      901 atattttaaa ggctatcggc aagagtggcg gagtaaggat agtttcctgc cctacctgcg
      961 gaagaactag gatagacctt ataggtcttg caaaaaaggt cgaggaagcc accaaggata
     1021 tagacaagga tatcaagata gccgttatgg gctgtgttgt gaacggcata ggcgagtcgg
     1081 gcaacgctga tataggcatt gcaggcggtg acggctgtgc ggtgatattc agccatggtg
     1141 aaaagctcag aaaggtatca gaggaggagg ctttgcctgc gcttctggcg gaaatagaaa
     1201 aactttaaaa ggatatacca taaatgaata catacaccat tgaaagcttc tttgagaact
     1261 ataaggagct tataccctct gtgatagaaa agggcaggat attaaggctg ggctataacg
     1321 aagaaaacgg cacttttacc tgtcaggcgg cttttgacaa gctggtgcct tttgacagca
     1381 cagtggaatt tgaaatgaaa atgagggcgg ctctcggagc gggaaagttt attttgcaac
     1441 tcaaatatac ccctgatatg ctttgtgcgg agtattttcc tgagctttgc aagttcctta
     1501 aaagcagatt tcctcttgtg aacggctttt ttgacaacgc tgaggctgtt tatgaaaacg
     1561 gcattttcac cgtggatata aaacacggag gcgaagcgtt gctgaaaaag gcgggcatag
     1621 aaacggcttt tcctgcgctc accatggagc ttttttccgt aagggcggat ataaggctca
     1681 caggtgtgct tgaaactgat atggaagagc atcagcgtga gcaggaggag tttttgaact
     1741 ccctccctgt gccgaaaata gaccctgcac agcctgagaa aaaggcggct gttaccacta
     1801 atactgcaag cggtgcttct gtggatttcc gaactgcaaa tgtagacttc acaaggttct
     1861 cgcttctgtg cgaggacgct cttgtgctta tgggtgcgcc aatatcagga aacgagcagg
     1921 tgatgcagat gaaagatatc cctgcggatt accgtggacg tgtggtagtc tggggcgata
     1981 ttttcggacc tgtggaaaca aaggaaacaa agtcgggtat gcttatagca caccttaatt
     2041 ttaccgacta tacttcctca aactctataa agttcatagg ctcaaacaag aatttcagaa
     2101 acatgaaagg cttgaaaagg gcagtgcttg aggcaatgct ggaacacctt aaggcaggca
     2161 agaccatact tgtggcaggt gagatagagg aggacgattt tgaccactct ataaacatca
     2221 agcctgacag cattatggtc gtaaagcgtg aaaaggaaaa ggatacctgc gagcataagc
     2281 gtgtggagct tcattgccat accaatatgt ctatgatgga cgctctcact cctgcgggca
     2341 agcttgtgga aagagcctat tcatggggac acaaagccct tgccataaca gaccacggtg
     2401 tcgttcaggg ctatcctgac gccggaaata cctgtatcgg catacgcaag ggtggcggtg
     2461 acttcaaggt gctttacggc atagagtcat acgaggtcaa taatgacgaa aagatattcc
     2521 gtggaacgga caagcgtgag cttacagacg agataatctg ctttgacctt gaaactacgg
     2581 gtacaaatcc taatgaagat aggatcatcg agataggcgc tgtcaagctg agagatcttg
     2641 aagttgtgga caagctcgat attttcgtaa accctgaaag acctatccct gagtttatct
     2701 caaacctcac ccacatcact gacgatatgg taaaggacgg tgcaagcgaa agggaagctc
     2761 tccttaaatt caaggagttt atcggcgacg accctgttct tgtggcgcac aattctcagt
     2821 tcgatacggg ctttatatcc gcctgtgcaa aaagacaggg catcgagata aaatattctt
     2881 ctatagacac tgtgccaatg tcgcagataa tgcttccgga gcttgaaaag cataaactca
     2941 actatgtggc ggagcatttc ggacttgggg attttcagca ccaccgtggc tgtgatgacg
     3001 cagaggtgct tgcagggata ttcataaggc tgtcgaaaat gctcatggag cagtatcctc
     3061 tgcttcttat aacagtggat atgataaaca gcttgcttgc aaatgaaaat caggtgcttg
     3121 caaagccgac ttatcaccaa attattatcg taagaaataa tacgggtctg aaaaatctgt
     3181 ataggcttat atcagactcc aatcttaaat atttcaaaag acgtccaaga ataccaaaat
     3241 ctgagcttgt aagacgccgt gagggtctta tcctcggtag tgcctgcgag cgtggcgagg
     3301 tcattcaggc ttatcttgag ggcagaaact atgatgagat caagcagata gcaagctttt
     3361 atgactatct ggagatccag cctgacggaa acaacaaatt tatgctcacc accgaaaagc
     3421 ctccttatga caggatacac acagcggagg atataaagga tataaaccgc tttattgtaa
     3481 aacttggcga ggacttgggc atacccgtct gtgcaacagg cgacgtgcat ttcatgaaca
     3541 agaccgacgc acagttcagg tcggtgatac aggcttcaat gggcttccct gacgccgata
     3601 ctcagcctcc tttgtatctt aagaccacaa accaaatgct tgacgagctg agctatcttg
     3661 gcgaggaaaa ggcatttgag gtcgttgtaa cgaatacaaa caacgtggct gatatggtag
     3721 accctgacct gaaagcgttc ccgaacggta cttatacgcc ttttatcgaa ggctcggttt
     3781 atcagcttca gtatatatgc tggaagaaat gctgttccat ttacggcgac tgcgaccctg
     3841 aaactataga agttcctgag ggcgaggacg ttgacgtatc aaaatatttt gagggacaca
     3901 ttccagacct tgttttcaaa agacttaaaa gagagcttga ctctattatc aagcacggat
     3961 ttgctgtgct ttacatgata tcgcagaagc ttgttgccaa ctctaacgag aatggctatc
     4021 aggtaggttc aagaggctcg gtaggttcgt ccttcgttgc ctccatgtca ggtatctctg
     4081 aggtaaatcc tctagtgcca cactatgtct gtcttaactg ccaccacagc gagttcatta
     4141 cagacggctc ggtaggctca ggctttgacc tgccaccgaa aaactgtcct gtgtgcggag
     4201 agaatatgca ccgtgacgga cacgatatcc catttgaaac gttcctgggc tttgacggag
     4261 ataaagcccc tgatatcgac ctaagcttct caggtgacta tcaggcaaag gcgcataaat
     4321 atacggaagt tcttttcggt gaggaccatg tgttcaaagc cggtactatt ggcatgatcc
     4381 aggaaaagac agctttcgga tatgttatga aatatcttga cgagcgtgga aaaacagttc
     4441 caaaggctga gatagaaaga cttaaactcg gctgctgcgg cataaagaga acgacctctc
     4501 agcacccggg tggaatggtc gttatcccta gcgactatga ggtctatgac ttcacacctg
     4561 ttcagcaccc tgcggaaaaa gtggactctg acatggtaac tacccacttc gacttccatt
     4621 ctctccacga tactatcttg aagcttgacg agcttggtca cgataacccg acaatgtata
     4681 agtatctgga aatgttcaca ggacgagata tcctcgattg tcccatgtct gatcctgcgg
     4741 tatattcgct gttcacttcc cctgagtcac ttggagtgac ggaggaggat ataatgtgtc
     4801 agacgggaac gctcggtata cctgaaatgg gtacgccgtt cgtaagacaa atgcttcttg
     4861 actgtcagcc aaaaaacttc tctgaccttt tgcagatatc aggactttca cacggcactg
     4921 acgtatggct cggcaacgca caggagctta tcaaaaacgg aacctgcgat atctcaaatg
     4981 ttatcggtac tcgtgacagt atcatgacat atctgcttta tcatggcgtt gagccaaagc
     5041 tgtcatttaa gataatggag ataacccgta agggtaaagc tccgaaactg ctcacagagg
     5101 aaatgaaaga gaatatgcgt cagcatgacg ttccggagtg gtatatcgac agttgtttga
     5161 agataaaata tatgttcccg aaagcccatg cggcggcata tgttacggca gctataagac
     5221 tttgttggtt caaggtgcat gagccgcttg cgttctatgc aacttatttt acggtcaagg
     5281 gcgaggattt tgacgcagag atcgctctca agggaaaata tatcgtcaga agcaagatag
     5341 aagaaataaa gaataaacca aaagacgagg tgtcgggcaa ggacagcggt gtgcttgaaa
     5401 ttctcatgct tgtgaatgaa atgctcagcc gtggatatga ctttttgcct gtaaacatca
     5461 aaaaatctca cgcaactatc tatcagatag aggacggcaa gataaggctt ccgttctgtt
     5521 cacttaacgg agtgggcgag tctgcggcga taagcattta cgaaaaggcg cagaacggcg
     5581 agtttatctc tatcgaggag ttccagcagc agagcggagt gtccaaatct gttatcgaaa
     5641 ctctggagaa aaacggagct ttcggcgata tgcctaaatc taaccagata tcgttgttct
     5701 gatagttact atgcacaatt tcatactttg cccttgtaac actattgaca aacaagggcg
     5761 ggtgtgataa aattatttgt gttgatacta tgctatcact ttactacgat aaagtaaagg
     5821 cattacataa caacataaaa agtacaatct ataatttata tacaggaagg aaaagtgtat
     5881 gaaaagatat gatcttataa cccctgaggg tacgagagat ctgctgtttg agggctgtct
     5941 ggcacgaaga atggtggagg acaggctgtc aaagctgttt gagggctttg gctacagcga
     6001 ggtggtaaca ccgggaatag agttttacga tctgtttatg ggaagctcga gaaacttcca
     6061 gcaggagagc ctttataagc ttacagactc aaagggcaga cttatcgtta tgcgtcctga
     6121 ttccacgata cccatagcaa gacttgcttc cacaagacta aagggagcaa agcttcccct
     6181 tagactatac tacaatcaga gcatttttga aaataactcc ctgctcaaag gacgttcaga
     6241 cgaggtagtt caggcaggta tcgagcttat tggtggagaa aattccaaga gagcagatta
     6301 cgaggtgcta tgcactgctg tggaggctct ttcaagcttt gataaggaga acttcagact
     6361 tgagataggt catatcggat atttcaagga gcttgtggct cagcttgacg ttgacgacgc
     6421 agtaagagag gagataagac ttcttatctc tgcaaagaac tatcctgcac ttaatgatat
     6481 ccttgacgag gtgggcgaca atcaggtgac taacgcgctc agacagctcc caagactgtt
     6541 cggaggtgtt gaggtgttcg acaaggctgc tgatatttat actgacgata agataacagg
     6601 catactttat aaccttaaag aggtctacac cagactttca agccttggtt atgagggcaa
     6661 gatatctgtt gaccttggca tcgtgagtca tgcggattac tacacaggca ttgtgttcaa
     6721 gggctatctt tcagaggtcg gtcagtcggt gcttaaaggc ggaagatatg acagacttct
     6781 ggcagagttc ggcaacgatt gcccggcggt gggcttcggc ataagcgttg actctgttgc
     6841 aagacatatc gaaaagctag gcggagcacc aaagctcaga actcctgacg cagttgtgtt
     6901 tggcgaaaag ggctacgtgg tggaagccat ggaatatgct cagacgctcg taagagaggg
     6961 ccttacagtt gaaaactcgc ttttcaacac actcgatgaa acaaagtctt acgcaaagtc
     7021 aaagggaata aagaagcttg tggtcgttgg cagcgagata acagagcttg atatataagt
     7081 ttggaggata agttaaaatg aacagacccc ttaggatagc tcttacaaag ggcagacttg
     7141 aaaaggatac agtcggtctt ttagagaaga taggctatga ctgtaccgca ataagagaaa
     7201 agggaagaaa gcttattctt cctgtacctg acggaaacct tgaagttgtg cttgcaaagg
     7261 caaacgacgt tatcacatac gttgagcatg gcgtgtgcga tatgggcgtt gttggcaagg
     7321 acaccatcat ggaaatgcag ggcaagttct ttgagcttgt tgatatcggc ttcggcagat
     7381 gtaaatttgc acttgccaca aagaaaggct caaagttcta cggcggattt gatgtcaaga
     7441 cagttgcaac aaaataccct aacatcacaa gaagattttt tgaagcaaag ggaatggacg
     7501 ttgacatcat caagatagaa ggctcagttg agcttgcacc gctccttgag cttgcagacg
     7561 gtatcgttga tattgttgaa acaggcacga cacttaaaga aaacggtctt gaagtcatcg
     7621 aggacgtttg ccctatctct gcaaggctta tcgtaaatat ggtaagcatg aaaatgcgcc
     7681 agcaggagat cgagaatttt gtaaagcttg ttgaagaaaa tatcgacaag tagtttatat
     7741 aaacggaaag gacagtattg aaatggctga aataaagaag atttttgtca acggcaaaga
     7801 cgaggtagag tttttcaagc agcttgaaaa gagaagcggc gaaacaaata agaaggtcac
     7861 agccgttgtt aatgaaataa tcgaaactgt cagagagggt ggcgacaacg ctgtaaaggc
     7921 ttatacagaa aagtttgacg gcaagctccc ggagtattat gaagttccga gagatgttat
     7981 aaacgacgct ctcactgaag ccgatcagga ctttgttgac gcaatgctca acgcagttgc
     8041 gaacatcaca gattttcacc aaagacagaa gtcacagagc tttataaacg ctaaggataa
     8101 cggctgtatt ctcggtcaga aggtaagagg acttaacaga gttggtcttt atgttccggg
     8161 cggcacagca gcttatccgt caagcgtgct tatgaatgca gttcctgcaa agatagcagg
     8221 cgttaaggaa ataatcatgg taacacctcc tctcaaagac ggcacaccaa ataaggatat
     8281 tcttgtggca gcagctctct gcggagttga cagagtgttc atgagcggag gcgcacaggc
     8341 tatcgctgcc cttgcctacg gcactgagga gataccaaag gttgataaga tagtaggccc
     8401 tggaaatatc tatgttgcaa ctgcaaagaa gcttctttat ggtgttgttg atatcgacat
     8461 ggtggcaggt ccgagcgaga tacttgttat ggctgacgaa aaggctaacc ctaagttcat
     8521 tgccgcagac cttatgtcac aggctgagca tgacaagctt gcttccgcta tccttgttac
     8581 aacaagcgag agcattgcag acagaactat cgaagagata aacagacagg tgcagagcct
     8641 ttcaagaaaa gagataatcg aaagctctct tgaaaattac ggtgctatcc ttatctgcaa
     8701 tacaaaggac gaagcttgcg accttgcaaa cagatttgct cctgagcacc ttgaagtgct
     8761 ttttgaaaac cctatggagt atcttggcag acttgacaac gcaggttcaa tattccttgg
     8821 tcagtatgct tcagagccgc ttggcgacta ctatgcaggt cctaaccacg ttcttccaac
     8881 aagcggcaca gctagattct tctcgccact ctcagtaaac agctttgaaa agcgttcaag
     8941 ctttacatat tacacagagg acgctctcag agaagcaaag gacgatatcg ttcttattgc
     9001 agaaaaagag ggtctgacgg ctcacgcaaa tgcaataaag gtaagatttt aagcggtaaa
     9061 ggtgatgaaa atgcgtacag cggagataaa aagaaaaact aaggaaactg atatagaggt
     9121 gttcgtgaag cttgacggtg agggcaaggt aagtgtaaac acgggcatag gcttctttga
     9181 ccatatgctc acagcccttg gcgttcacag cggaatagac atggacgttg agtgcacagg
     9241 cgacctttat gttgacggac accacacagt tgaggacgtt ggcatatgcc ttggcaaagc
     9301 ctttgcggaa gcattgggcg acaaagcagg cattgcaaga tatggctcgg ctttcgtgcc
     9361 aatggacgaa gcgctggcat tctgctcact ggatataagc ggcagacctt tccttgcgtt
     9421 caatgctgag ttccatgacg acaggatagg tcagtatgac acctgcctga cggaggaatt
     9481 tttcagagcc tttgctttca actcaggcat aacactgcat atccgtgagg agtacggcaa
     9541 gaacgaccac catattgcag aagcaatgta caaggcagtg gctcacgctt tgaaggacgc
     9601 tgtaaaaata acaggaacag gagttttatc caccaaagga gtgctttaaa tgatagcaat
     9661 aatcgactac ggtgcaggca atatattctc tgtaaaaaac gctctggact atcttggctt
     9721 tgagaacaag ctcacagaca aaaaagaaga tatcgaaaaa gccgacgcaa ttatcctccc
     9781 cggcgtggga gcgttcccat gggcaatgaa tatgctcggt cttagcggtc ttatcgacac
     9841 tatcaaggag caggctaaaa taaagccttt tcttggcata tgccttggaa tgcagcttct
     9901 tttcgagaaa agctatgagt tcgaggaatg caagggtctt ggtcttattg gcggctatgt
     9961 tgacaagata aacgagcctg accttgttat cccacacatg ggctggaaca agcttgtgta
    10021 taatcatgat tgtcctcttt ttgacggtct gggcgatgac gagtatgttt atttcgtaca
    10081 ctcatataaa gctttctgcg acaacaaaaa tctgtatgcc tactgcgaat acggctcaaa
    10141 agtccctgcg gtggtatcag acggaagata tatcttcggc tgtcagttcc accctgagaa
    10201 gagtggaaaa accggtctta aaatacttga aaactttgcg aaactgtcac aggaggtaaa
    10261 ataatatgct tgcaaaaaga attataccat gccttgatgt gcgtgacgga aaggtagtaa
    10321 agggagtaaa tttcgagggc ataaaagaag tcggcgaccc tgttgaatgt gcatatgaat
    10381 acaatttgca gggtgcagac gagatagttt tctatgacat aacagcctcc cacgagggca
    10441 gaggcgttat gctggacgtt gtacgtgaaa cagccaaaaa ggtatttgta ccgctcactg
    10501 ttggaggcgg tatcaaaact gttgacgata taagagatac tctccgtgca ggcgcagaca
    10561 aggtgtctgt gaactctcag gcagtgcaga atcctcagct tataaaagac ggcgcagata
    10621 ttttcggctg tcagtgcatc tgcgttggcg ttgacgcaaa gaaggtcggc gacaacaagt
    10681 ggacggtcta catcaacggc ggaagagtgg atatgcgaat ggatcttatc gactgggtga
    10741 aaaagtgcga acagcttggt gcaggtgaga tatgccttaa ttctatcgac acagacggtg
    10801 taaggggcgg ctttgatctt cctatgctga aagctgtgtg cagtgctgta aagatacctg
    10861 ttatcgcaag cggcggagca ggaaagcttt cagactttgc tgacgctttc cttgaaactg
    10921 actgctcagc agctcttgcc gcaagccttt tccacttcaa agagctgaca gttggacagg
    10981 taaaggacga ttgcaggagc aagggaatta tcgtgaggtg agcataatga ttgttttcaa
    11041 tatcgtattt actttgttta tgccagtgct tatgatattg tgcggctatc tgatgtataa
    11101 gcatacaccg aaagatatca actgcttggt gggctatcgc actgctatgt caatgaaaaa
    11161 tcaggaaacg tgggattttg cccacaatta ttgcggacgt ctgtgggtaa aggtcggagc
    11221 ggtcatgacg ataatttcgg cattggtctg tgttccatat tgctttatga gcagcggcaa
    11281 tgcgactgtg ataatgtgca tagctgaagt gatacaggtg gtagtttttt gctggacgat
    11341 agtaccaaca gaacgagcac tcaaagataa tttcgaccaa gacggcaaca gaaaggacaa
    11401 ataatgaacc caataaaagt tgatataaac gatctggata aatattttgt aaaaagcgag
    11461 cttacccctg ctatcgcatt tgatgtggat acaaaaactg tgcttatgct tgcttatatg
    11521 aataaggaga gcctgaaaaa aacacttgaa acaggctata catggtattg gagcagatca
    11581 agacaggaat attggaacaa gggtgcaaca agcggtcatc tgcaaaaggt ggtttctatc
    11641 tatgcggact gtgataacga taccctgctt cttaacgtaa agcagacagg tgtagcctgc
    11701 catacaggct catatagctg ctttttcaat gagatatata atacggagga agaataaaat
    11761 gacagtttat caggaaattt tcgaggtaat aaaacagcgt aaagctgact atgaggcagg
    11821 caaggcgcag gagaacagct acacagctta tctttttgac aagggcgtag acaagatttg
    11881 caaaaaggtg ggcgaagagg ctacagaaac agttatcgct gctaaaaacg gtgacaatga
    11941 cgagctgaaa aacgaaataa acgaccttct ctatcacgtt atggtgcttg cggcaaatca
    12001 gggacttgag tggtctgacg tggaaaaggt ccttgacgag agaaacgaaa aaataggcaa
    12061 tctgaagaaa tttcatcagg tagacaagaa cacctgatta tatgtatcca aaggctctgc
    12121 gttcatgcgc agggcttttt ttgctttatg agaggatttt cagaaattta agcacacaaa
    12181 agccttttgc cgaatagaat atagcgaagt aaaaaaactt ccatgatttg atttatagga
    12241 ggacttacta tgagcgattt taacggcaaa cctttcaagg aagcaatgtg tatcgaggca
    12301 atgcgtgtgt ttgattcatg ctcgtcacag gattgtctgg aggatcttgc gtttacattc
    12361 agccaggcag accagaccgt gataaacgct gcggcgtata tcaaaagcac ttgtatcgac
    12421 gtgacagacg taagctttgc aatatctccg gtggcattca accacggctt ttacgcagtt
    12481 gacgttactt acacattcag ggcg
//
LOCUS       DAWEMR010000069        12158 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2216_length_12158_cov_10.128150,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000069 DAWEMR010000000
VERSION     DAWEMR010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12158)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12158)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12158
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2216_length_12158_cov_10.128150"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3663 a         2479 c         2596 g         3420 t
ORIGIN      
        1 tgtaacgaca gcgcgatcat gacgcttgcc gaacctgggg ctatggggat gggcaacctt
       61 atggaattta ttacagcgga aggaaaggag ttctcgcttt tcttttctga ggaaatgcca
      121 tactcaaaag tcaaggagta ttttccagcg ttagatgatt gctattggaa tggtcctgaa
      181 tcagatgaat cctgcagaac tgagttcgtg ttctatctca gtaaggacga aaggaacttc
      241 aagcacacaa agcctccgaa aaattatacg cacctgtatg aaggttttgg aaatcacata
      301 tgtatacgta aagattatta tcctgtggtc gagccgatca taagagatct tatagaaaag
      361 aatgagcttg taaattggta cacgaggact ggagaaataa tagacgcaat aaaaaccctt
      421 acggcagaaa agaaagaagg aaaaatgcaa tgcgattagg agattttttg gattgttttg
      481 accccagcag tgtagtagag atagcgtgta aaggcgaacc tgttaggaaa tgcacagtgt
      541 cggccgcatt atcggacgac agatacccgg ccatagacta tgcggtcttt cctggcagtg
      601 ttactattaa ggatgatatc gtacatatac cagtgatttg aaaattgacc tgggggacgt
      661 tcagaaagtg tataaatact tatttcgctg aacacgaaga aaatggagca gtataatgac
      721 caaaagtagg tggatatttt gtcagaatcg tcgaattttt ttagcaccca aaaaaattat
      781 acgtcagacc tgcatttttt tctctaatag gatcgtttgt atatataatg ggaatactat
      841 aagggataaa acagcaggat ataaaaacaa agaatgcgtt tttatgcttg attttttcga
      901 gcgattgtat tataatagca ttgaaaggag ctgataatcg atgattaaag ttttaaaaat
      961 atctgccgaa ggccttccac tatacgatgg taaatgtgaa atcaacttta ttaacacaca
     1021 aagaacaacc tcaggtgata ccgaaggaat gtttaagctt tcttcttcaa acgctaacag
     1081 gctgtataca aataatatag aaagtttttt tggaatcaat gcttccggaa agaccacaat
     1141 tttaaggctt atttcttttg ttttttctgt tttaaacaac gagcaaataa ataatataaa
     1201 atactgcgga atacttgggg aaggcaacga gcagaatgag attgttttta acattgtatt
     1261 tgtaaattgc aatactgtaa atctgctaca cactgttatt aaatatgcag atgaacgcta
     1321 tacaataata gaggaatata ttaaatccag gaagttgctc gcatctgaca acaaaacaag
     1381 catttttgac ttttcttcgg caacaacgat tatgaaaaga aaagatgatg aacaatacct
     1441 agtagacgat atgagcatta tcgtaggata caaaaaagca gaaaaaacaa cgatcgtatt
     1501 tactgatatg cttgaaatca cgaatataaa cagactcaaa ctcttaaagg aatatcctct
     1561 aagcgttctg tcttattttg attccaagat agaatatata aatactaggg aaatcaacgg
     1621 aaaaacggtt atatatttta agtttcgagg cgaaaaagag agaatattat atgatcttgc
     1681 ggagcttaat acgtatttat cttcaggaac aataaaagga ataaatgtct ttggaagagc
     1741 tatatctctt tttgtaactg gaggatatct tataattgat gacctagaga accacttcaa
     1801 tcatgagatc gtatcaacgt tgatacgatt tttcacggac aaaagagtaa acccgcatgg
     1861 ggcaaccctt gttttctcaa cacattactc cgaactcctt gaacacataa aaagaaatga
     1921 ctgcatttat attgtggaaa acaaggataa aatcacaata actccattaa atgaacgaat
     1981 gcagagaaat gatgaaaata aagtacaatc gtttaaaagt ggactcatag gcaatactgc
     2041 tccttcatat gaagcatata tccagctcaa aaagagcatt ataaacaaag cggaggcaat
     2101 cgtcccaagc cctgtcagac catagtttat tcatcgttca cctcgataag cttgtgctac
     2161 ttgccgtttc agcttgaaag ctgatccata gacctgtcga tcatagcaag atattactca
     2221 ttgtgtgctg ccttcataat ctgattgtat tgctcaaggc tcattataac aacaatttgc
     2281 cattttctta cttttaccat tccgcaaatt tctcttgttg cacagtttgt aataaaaatg
     2341 tgattatttt atacttctgt tttgaaaatt gattcccgtg gcctgaattc cacaacagtt
     2401 gacaaaactg caaaaatatg atgtaatttt caggtaataa tttaagggca atgacgctcc
     2461 gtgtaatttt cacggggcgt tttttatttt tttgtggggg tgttttctgt gaaagtcaat
     2521 aaaaagaatg gcagaagtgc gctcaaaagc cgcaatatca ccatttgtgc agttgtggtg
     2581 tttcttctta ttacggctct tactttaatg agcagcagag atatgaccca gtgtatcgac
     2641 agagaaaata ccgcacaatc gaacagggga gagctttctg accttggaca ggagcttgcg
     2701 gacgcttccg attatcttac cgatgaagct agaaaattct ccgtaacagg ggatatcgaa
     2761 cacctttata attattggta tgaggtctat gaggaaaaga ctcgtgatcg tgttataaac
     2821 agcctttccg ccattgaccc tcctgagaac gaaactgcgc ttcttgcgga agccaaaaag
     2881 tattctgata ctctcataaa gaccgaaaca gtgtcaatga atcttatgtt cactgcaaaa
     2941 gggataacag caaaacagtt tggtgacagc aaggacggca gattggctga gtatgtttcc
     3001 attgtggaga atacgaccct gcctgaggaa tacagtgatc ttagtcctga tgaaatgaag
     3061 gaactttccc gtgagatact ttatgacagc ttttataacg actccaaaac tatgataatg
     3121 tcgcctatag aacgctttcg tcaggctctt aataaacgtc ttgacgcaga ggtggaaagc
     3181 gctgccgcag gacgtgaaac tgcactggtg atacagcttg tgtgttcagc gagcgtactt
     3241 gttattgtgg gcatagtgct gatagttttt gaaagccttt atgtcagacc tataaacgac
     3301 tactccgaga gcctttcaaa gcgtaatgaa aattctgccg aatttgacct ttcaaacgtc
     3361 cgtgtgtccc caaagggagc atatgagctt tttcgttttg gcgagctttt caaccgactt
     3421 tcgcagatac ttcaaaatga gcttaaaaag cgtgaaacag ccgaggtgta aatgtggcag
     3481 gctaaagaag aagcagacag ggcgaacagc gcgaaaagtg attctgcggc aaagcggcat
     3541 gaaagcctat gagataagcg aggagcttgg ctatttcagt gcagactatt tcacaaaggt
     3601 gttcaaagag ataactggcg tttcccctgc acaatataag caatcattgt gacttgtcta
     3661 aaaatatagg gttttctctg atttttagtg tttttatctt ttgacccctg tgctatacta
     3721 taagcacagg aggtgataga aatggacaat ttcgttatta ccatagcaag aggcttcgga
     3781 agcggaggaa agcagatcgg acttgctctt tcaaaacagc ttgggatacc ttgctatgaa
     3841 aaccagatac tcagcatggc ttcaaactac agcggaataa acaaggatct gtttttttca
     3901 ggtggacgaa aagctgaggg gctaccacct tatcaaaagg cttatgaagt ctgcgaataa
     3961 ggacgatatc gttgagccga cggacaagag cttcatttct gacgttaatc tttacaatat
     4021 tcaggcgaag ataatcaagg agcttgccaa gcagcaatcc tgcattatca ttggaaaatg
     4081 tgcaaatcac ctgctgcgtg actatgacaa caccataagc gtgtatatag aagctccaag
     4141 agctttctgc gtaaaaaatg ttattgaacg ccttggtttc accgaggaag aagcccacag
     4201 aatgatctat cagacttctt acttctctcg gccttgcaat aaccttcgga tttatgaggg
     4261 tcataaacat ggctcacgga gagtttgtga tgataggcgc atatacaaca tatgtcgttc
     4321 agcagatatt cgtaaaatac tgtcccgaaa gcattcgtga cgtttattat tttgttgcga
     4381 tacctgccgc attcggtgtg acgttcatcc ttggaagctt gcttgaaaag tttatcatct
     4441 cccgattata cgggcgagag atagacggct tgcttgcaac aggggcataa gccttatttt
     4501 acagcaggcg gcaagaagca ttttcggcac acagggtgta aacgtaacag caccgtcatt
     4561 cttaaacggt ggaataaaga taggcggctg tacattctca tacaacaact tttcatcatt
     4621 cttcttgtgg cactttgtct gcttctggtg tggtttataa tgtataagtc aaacttcggt
     4681 aagcagatga gagctgttat gcaaaacaga acaatggcga aatatatggg cataaactca
     4741 cgcaaggtgg ataacattac ctttgctatc ggcttaggtc ttaccggtat cgcaggctgc
     4801 ttggtggcgc ttcttggttc tatcgactcg actgtgggtc agagctacat agtaaacagc
     4861 tttatggcag tcattctcgg tggtgtcggc aagcttctcg gcactgctat cggctctgtg
     4921 ataataggct ctgcaagcat ttttacagag aattatactt catcaactat cgcaaaagct
     4981 attgttctgc ttatcgttat tatctttttg cagaagcgtc cgcagggact tttcgttatc
     5041 aagagcagaa atcttgacga gtaagggagg tgtttcggtc aatgaaacta agcagatttt
     5101 tcagaaagag gggagcattt ttcagtggga gtgccgcttt ttgatatatt tttaataatt
     5161 attaacaaaa tatcacaaaa ttactctata aatagttgta ttattaaatc atataattat
     5221 gacggtagaa aatgccgtta aaaataacgg aggaaagaat atgtcttaca acgaattttt
     5281 accaagctac tctattggcg acgactgcta caaagaaata ccttatgtta cccgacgtta
     5341 tggaaaaacc gctgttgtta tcggtggcaa gaccgctatg gaaaaggcta agcctgaact
     5401 gcttgcggct ctcaaaggct ctgaccttga gataaccgac ttcatatggt atggcggcga
     5461 ctcaaattat gagaacattg aaatgcttaa agctatgccg gaagtgcaaa atgcagatat
     5521 ggtctttggc gttggcggcg gacgtgccgt tgatactgta aagacccttt gcgacagaat
     5581 tgacaagccg tttttcactt tcccgaccct tgcttctaac tgcgcttcct gcacggcaat
     5641 atctgttatc tacaacgctg acggcacttt caaggaatat gcttacctca aagcacctgc
     5701 gctccatact ttcattaaca caaaaataat cgcagaatca cctgaaaagc ttttctgggc
     5761 tggtatcggt gacgctctca gcaaagagta cgaggtggtc tttgcctgcc gtgataagga
     5821 tatgacccat acgcctttgc ttggcagagt tcttgcagag gcatgcacaa aaccgcttgt
     5881 atcctacgga aaaaaagcac ttgaggattg cagaaataac gtttcttcaa agaatatcga
     5941 gcagatagct cttgatatca tcattacaac aggtcttgtt tcaaatttta ctgttcatca
     6001 gaacaacccc gatccaaagg acgattacta ttataattca tctcttgccc attgcgttta
     6061 ctatggcgct tcgctgtttc caaagtgcga gcatgaccac ctccacggag agatagtttc
     6121 attcggcgtg ctttgcctgc ttacatatga cgagcagttt gaagaacgtc agcgcatttt
     6181 tgagttcaac cgcagtatag gtctgccatg tactttggac gagatagctc tgacagaaga
     6241 agatgtccca gccattgcac ataaggcggc aagcgttgtt gagtggactt acgtccctgg
     6301 cgaaccttcg gaagaaaagt ttatcaaggc gatacttgat accgataagg caggaaagga
     6361 atttctagca aaggcataat aacaaagaac aacagcatat ataaatgatg actgagccgt
     6421 atggctttgt tagaaataat aaaaatagga gagtgctttt tgaacaagta caacacagtg
     6481 gagattgcag cgtaaacggg aggtttacgg cgatctttaa aacctcccgc tgagaagcag
     6541 ttgacattta caacattcag gaggtaaaac aatgaataaa aatattgtga ttcgtttaga
     6601 gaaaaaagaa gaatactatg aagtcgaaaa ccttgtaagg gagagttttt ggaacgttta
     6661 ccgtcccggc tgtcttgaac attatgtgct tagtcagctt agaaatgacg ctgattttgt
     6721 gccgaagctt gactttgtga tgctgcttga cgggcaaatc ttttaaacga taaaaagcaa
     6781 ggctctctgc catataaacg gcagagagcc tttgttttat tctttcatca aagccaattg
     6841 cctgtcaacc ctctccttga ttttcctgcg aaaatcatat ggcgaaagca cctccaccac
     6901 agatccgaat gccaacaaac gtattattat ctcattttcg ttctgctcat catatttaat
     6961 agaaaccata cattcaccgc tgtcctcatc aaagaccgac tctttttcaa gctctgcaaa
     7021 ttcaagcata aaacgattta ccgcatttcg ctcgttaagt atctttattt ttgcctgctt
     7081 tttcttgtct gtgacagcta aaaagctctc aggcgttata tccgtcagct tagtgagtgt
     7141 tacctgcgac aggtttataa ttccgctgtc catcattctg cgcttttggt atcggttcac
     7201 atataccctg aacttgttgt ttttggcaga gtattcgatt tttgtgggca gaaaatagtg
     7261 tgtgatgcgg ttgtcttttc ttgtctggaa gcttattttt attagctttt tgccatggat
     7321 agccgagagt atatcccgaa aatgctgtat gtactgtggg tcatgaaaat catcgccgtc
     7381 agaatattgg tcaaagcagg tcaaaaagtt tctctgatat agctttttcg caccaagcag
     7441 ttccgtcaac tgcgactttt gctcagtgtc aagaaacaga ccgcttctcg ggtcgcttag
     7501 caccgcacaa agccacgctt tttcaagtgc cgtcagcggg atatgaggct cttttttcag
     7561 gatagatttg tatacgctgt cctctttttt cagcaaacca tatccgccct cgcctgtgag
     7621 cttaggttcg aggaaaagaa cactttcaga aaagccgttc tgcctgatga tatctgttat
     7681 ctcagccttt gtgacagtgc gctttttcag tattttctcc gttatgctgt aataggtgct
     7741 gtatatttcc gaaaacagtt tcattcgtaa gcacctccgt atatttcata aagccttgca
     7801 atatctgaat caaatttatc ctgcaattcc tcgtttgttg tttcaaatgc cgctatcctg
     7861 ccgatgaacg tttttatcca tgggaaggct tcgttagcgt caaaaaggtc aatgtcaaag
     7921 gagtaaagcc catttgatat ctttgcgaca gtaccgtttc ttttttcacg ctccaaacgt
     7981 tttaccacaa acatttcttt cgtttcgtct accgctattt ccatgtgaat atgctctttt
     8041 tgtccgtatc tgcgtgattt tccgaagcta gttccccata aaaacctgcg gttgtcctca
     8101 taatatctgt aatatgtatc atagtcagct accacatcaa ggggagtcac tttcactatg
     8161 gaatccaccc ttacagacat caaacgtttc tgctttgtga aatacatgat aagataattt
     8221 cttcctgtct gcaccgagca gtgtatcttc agcacaacgg ctgttatctc gtgctttttg
     8281 tcgttctttc ggcttacgca ggatagcaga acagcctttt tttgctccat tgcccccatg
     8341 atatcaatga gtatctcatc atcaagagta tgcaccatgt aagcgtgctt acgcacaaaa
     8401 atattatttt tcgcacccag cttatccatg ataaagctgc ccaccacgcc aaaaggattt
     8461 tcctcagaga agaattttat catatccccc aagcccttat actgacttac aatatcatct
     8521 gcgtaacagc cagttttggt gtataggata ttccttccac tcttgacctc cgacaccaag
     8581 ccctctttta catattcctt tagcttgata cggattatct gcacgtctat tatcatgccg
     8641 tagtttgcag aaatataatc tgctatctcc gataccgaca tggcgttatc ttcaagagcg
     8701 tccattaaaa taaagtgcag acggatatca ttgtccgtaa aggtctttga acgatagcac
     8761 tgatacagtg ggttttgaaa aatgttcccc gaatccacct gcacagatac tttctttttg
     8821 tgaccctcca aagaggtgtg aacgtggtct gaaagccagc tttctatcct gcgtttttcg
     8881 ttatcatagg tgcgcttgct ttttgcgtca aagctttctc ttgtgtgaaa tccaaagata
     8941 tagaaatcac gcacatattc tcttaccttc tcaaaatttt ttataagttc agaatatacc
     9001 gccattcaga acacctcctt tgataacatt ataccaaatc tatactccca ttgcaataca
     9061 taacaaaacc tcgcaccttt ttttatatga ttattttgta tctatctgct ataatacata
     9121 caacagcaaa agtaggaggc gcacaaaatg tttgaatatt atgataaaga taaaatgcta
     9181 gtaccgatca agatatggac agagtctata gacgatatcg aggaaagctg tcttgagcag
     9241 gcgataaacc ttgcaaatct tccgtttgcc catgaccata tcgcattgat gcctgatact
     9301 catacaggaa agggaatgcc tataggcggt gtgatagcct gcaaaaatac ggtgatccca
     9361 aatgcggttg gcgttgacat cggctgtgga atggctttcg tgcagacaga tatccctgtg
     9421 aacattcttc gtaacactgt aacagggagc ggcgagctta taaaggtgat agtaggaaat
     9481 atactccgta ctatcccggt gggctttagt cattatggca agccgcaggc ttctgccgtg
     9541 cttgataagg cgcaaagcga gatagaaaga tacaatgctg acagcgaatt gataaaattt
     9601 atcgagcagg gatattattc cgttggctca ctgggcggag gcaaccattt tatcgagata
     9661 caggaagacg aaaacggcat ggcttgtata atgctccatt cgggaagccg aatgtttggc
     9721 aacatgatag ggcagtattt caacaagata gcccatgaga tgaatgacaa atatttttca
     9781 actgttccct cagagtataa tttgccgttt ctccctgtgg atacagacga gggtcagaga
     9841 tatctcaact ggatgaacct tgcaatggac tttgcttttg aaaacagaga ggtaatgctt
     9901 gaaaaagtga agcatatttt cacagagcag gttgagaagt atacgggtat cacaccgaac
     9961 tatagtgacg agataaactg tcaccataac tatgccgcat tggaaaatca ctacggtgag
    10021 aacgtgtggg tacaccgcaa aggcgctatc catgcggctg agggcgaagt cgctataatc
    10081 ccaggatcta tgggttcaaa cagctatata gtcaagggca tgggcaaggc tgaaagcttt
    10141 ttgacttcat ctcatggtgc aggcagaaac tattcacgca caggcgcaaa ggagaaattc
    10201 tccgttgagt ctgttatggt agaccttagg caaagaaacg tggtgcttgg aaagaccagt
    10261 aagaaagacg tggctgagga gtgccgcttt gcttacaagg atatagatgt tgttatgaaa
    10321 aatcaaagtg acctgaccac ccctataaag aagcttttca cagtgggcgt tgtcaaagga
    10381 taggaggtaa tttatatgaa acttgcaact gcattggctg aacgttctga tatccagaac
    10441 agaataaaca cgatctcagt cagacttgac aataacgcaa aggtgcagga gggcgatgtt
    10501 cctgcggaag acccaaaggc tcttatggag gagcttgaca ggctgatagt ccgtcttgaa
    10561 gatctggtgg cacggataaa tcttaccaac agcggaacag tatatgaggg caagactatc
    10621 acagagcttc ttgcacacag agattgtctg aaaaagaagg taagggtatt gcgggatttt
    10681 ctgaataccg caagcggccg tgtcacaaga atgacaagaa cagagataaa aatagtcagc
    10741 actgtgcctg tgacagagat acagaaaacg gttgacggtc ttgcaaaaga actgagaact
    10801 gtggacgaga aaattcagga gctgaactgg acaacagagc tagtgtaata aaatgggtgg
    10861 gatatctaca gggtggatgt atctctatca gcagagaatg tgctgattat ggacagaaac
    10921 ttcgggcagg tttcagaggt tcaagtcctc atttgacaac gcattgccag caacgtaaca
    10981 tgcgtatcgt aacaaattct tattaccacc ttgcatcaac tgtagatagg gtaggaatat
    11041 gaaaaacggc atgaccttga tttggtcgtg ccgttttttt tatgcaatgt catcagaata
    11101 tttcgtaaaa tgtgcaaatg ctcaaattat tccctcataa aatgtgtgaa atcataaaac
    11161 attccctcat gaaatgtgca gagaccaccg caaacgtcta ttctacgccc ttttactcct
    11221 catgtaaaag gagcctttta tagctgcggt caatgcctat tgcaccaagg ggtgctgtga
    11281 taaggatcgc aagcaccgcc acagaaagca ctatctttcc gcagggcaga ccaagggaca
    11341 tcggcactga gccgatagcc gcctgaactg tcgcctttgg catatatgcc gttacacaga
    11401 aaagcttttc tttaagggtc agcttcgtgc cgagaagaca tatccatacg cccatggctc
    11461 tgaaaataag cgctccgaaa ataagaagca cagccgccac acctgccgca agggtgtagc
    11521 gtatgtccac tgccgcacca acaaggacaa aaagcacagc ctcagcggct atccaaagct
    11581 tgccgaactt ctctgaaagc cttgcagaca cgcttttgtc agcctttatt tttatcacgc
    11641 aagccattgc cacaactgca agaagtcccg atacagccac ataaggcttc aaaaatgttt
    11701 ccactgacat gagcaggaac gccaagccca agatagcgat gacctttgtg ctgtttctta
    11761 ttttgttacc tttgcggtag gatatctcaa aaatatagaa aagcacaaac cctgacagcg
    11821 cgccgagaag cactccgagg attattgaca cagggatatt tagaaaatcc gtcgcctttg
    11881 cagagccgcc ctgcgccatt gttacgaatg tggagaacag aacgataacg aaaatatcat
    11941 cgcatgaagc tcctgcaagt atcatttgcg gtatgctttt ttcagtgccg tatttttctt
    12001 ccataagctt caccattctc ggcactacca ccgcagggga taccgcacca agcacagcac
    12061 ccataacagc ggcttccgtg tggtttatcc caagaagcag cggagcaagg ataaaatatc
    12121 ccagtatctc aaagcttgca ggcagaaatg acatcatg
//
LOCUS       DAWEMR010000070        12019 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2245_length_12019_cov_8.692996, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000070 DAWEMR010000000
VERSION     DAWEMR010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12019)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12019
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2245_length_12019_cov_8.692996"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2709..2731
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3224..3233
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7653..7662
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10063..10072
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10722..10731
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3619 a         2385 c         1753 g         4199 t
ORIGIN      
        1 tgcttgactt tccacggaca gcagagttgt tttttatttg ccaacaatag ttttagtttc
       61 gtttagcctt agtaccaatt caaacttgtc atctacatac attttcatga atgtttccaa
      121 caaatcgttc attttaatgc cgttaatagc aatatctcag aaactaaaat tgtgtatttc
      181 aacaaaaaat acgctagaat tttgtgaaag atttttattt ttatatggtt gacaaacata
      241 taaaaataaa ttatacaata ataaaaggca ggtgagaaaa taataatgga tagaaaaccg
      301 ttcacaacaa caatagacag cgaaattcaa aatcagttca agtcaaaatg tgctcttaaa
      361 ccctaaatct tgattttcag cctaaaatat gctaaaattt tttattaaaa gtaattctaa
      421 ttaatcttag aaattggagg aaataaaaat gagcaaaata aaattaattc ttattgcact
      481 catgacaaca ttagcattgt cctcatgtaa tagtaaaaca acaagttcca tatctgacag
      541 taatttcact accactacaa caagtacaac aaccaccact cccacaacaa cttctcatac
      601 tttaacaaca actaaaccat caactaccac aactacttcc aaatcatcaa ctactaccac
      661 aacgactaca accacaacga caactacaac taccacgcat gattatagtt ctgaaataag
      721 tgctttagag caagaaaata atcgcctaca gagtgaaatc tccacctatc agaacgaaat
      781 aaacaatgag caatctgata tttccatcta tgaaatctac aaatcggatg ccgaagatga
      841 tgttgaggag gctaaaatac agcttgaaaa cgccaataag aaaatggtta aagtttatgg
      901 tgatggcggt tggactacag aagttgactc agaagcagtt tcaaaggctc aatctcactt
      961 agacgattgc caaagagttg ttgacgtgta caatgaactt atatcagaaa gtcaaagtaa
     1021 tattgattat tataacactt gtatatccaa taatcaaagt tccattgaaa gcaataatag
     1081 tcttataaac gattatcgta gcagataatc ataaaacagg aggtaatacc atgaagaaaa
     1141 tttgttccat tcttgtgatt gcaataggaa taacactatt tgtgataggt tatacaacaa
     1201 aaattccaag caaaaattta accacatttt caattttgga aggtgacaag tatagtgcca
     1261 ttgacgaata tgttggcggt gacgcttaca actatatcat aggagcttca cttgtcagcg
     1321 gtaaaatagc cgctgcgaaa attgagagag taattttcat atccactggc tcattaattt
     1381 tctccattgg cataattggt tttgcatttt catttaaaac caaagaaaag aaacctaaag
     1441 aaaaaaagga tgttggcgag cagggtgact tgtcacaaac taacgaataa attttacaaa
     1501 gttccacaaa atagtattga caaaatgggt atagtatgct atactataaa tgatgaaaga
     1561 ttatctctat catctctaat ttacgcttcg caatgtgcga cacagaaaca ttgtagatac
     1621 aattacgttt cacaatgtac ggcaaagtaa cattgtagta ttcaatttac gcttcgcaat
     1681 gtgcgacaca gaaacattgt agtgatgctg tcattttggt taatctgaag tgacagcata
     1741 ttttttgtat taggagtgtc aaaatatgac ggaacatggt atgtacttta ttacacccga
     1801 ctattatcaa cttattcgag atgtaggagg aacttggaat gattgcaagg aaagacccat
     1861 tgtttgtttg attaagtcca ccgaaaattc caaattgtat tgggcaatac ctgtaggcaa
     1921 agtaaatcat cgtgacacta aagctattaa tcgtatttat tcctatatga acaaagatcc
     1981 aagaaatatt gcttcttgct tttatcacat tggcaagaca accaccaaat ctattttctt
     2041 tattagtgat gcttttcctg taacagatgt ctacatagac agaatttatg agggttatga
     2101 taaacaacaa tatgtcattg aaaacaacaa tcttctgtct gctctgaaat ataaactcca
     2161 aagaatttta agttatgaaa atactaatcc aaatttcttc cgtcaacata ttaccgatgt
     2221 taaaagaaaa ctgttagacg aaattaacaa ttaaacaaaa gaggtattct tatgtccgaa
     2281 attaaatcaa taacagacca agaaatatta tcatactggg actcaattaa atccgtaaga
     2341 ggagttgcta ttaaactcgg tatctcgtgg caaagagtta ttaaaagtct ttctagttta
     2401 ggtattatag tcaacaatac ccacgccaaa atcactcaat accacaaaga agggaagtcg
     2461 gctaatgaaa ttgccgactt aatgaatatg aatgttaatg ttgtgaaagc ctatctccca
     2521 cgcaacagac ctcaatacaa ggttaatcaa tctaaaaatg ctctagcagt acagaggagt
     2581 aaagaacgcc acaagaaatg ctaaagggac ttttaaaagt ccctttttat tttacatact
     2641 tatccacaac ttccttgccc acttccattt ttaacatttg ctcttttacg agtctgctat
     2701 cgcagccann nnnnnnnnnn nnnnnnnnnn ngctcagtta tcctcaaatc agaatgtcct
     2761 aagctctgac aggcaatacg caaatctcta ataacatctt cactgccttt ttgaatacaa
     2821 ctaatataaa cggaatgtgt ctgcctaaag ctgtgagtgc tatatttacc ttctatgccg
     2881 tgtttggcgg ttatattctt tagaaatgtt gtaacggaat taagttccat aggagctatt
     2941 ctgagtagtc tgccgttcca atcatacttc tcattagtat atataatttc ttcttctccg
     3001 tcctcattca agaaaatgtc ctcaatatac ttcctcttac gttctccact ctgaaaaata
     3061 taatcttctg ggtcaagtcc ataatacttg attataaaac tcagcatttt cttcacagta
     3121 tcacaaagcc atgccgttct ccatttgtcc gtcttgtcct cttgtaatgt caagtaatct
     3181 acaattttgc cgttgttatc ggttaaatcc ttgaccctca aggnnnnnnn nnngtaacga
     3241 tagcctgagt tgcaagcaaa aattataata tttgccttaa aatatttttt actctgaaac
     3301 aaatcttcca aaatcacatt tagatcatca ggtctgaacc agcttgcaga cttctgcctg
     3361 cttgccgtat gttttgtaat agcatttcta tgaccttttt ttcgctttgg ctgttttgtt
     3421 atctgtattc ctgtcggaag tctatccgat aaatcgaaaa ttttgcaagt ttgagccgta
     3481 ctaatattca ttttcattca ctcccatcat atacacaatg taaatattat tcctgctatc
     3541 aacataacgc ttgtaaagag caagccaaaa ccaccataga caacgttctt cactatcatt
     3601 ctaacttttc tctggcgttc ttctctgagc ctttgacggc gttttgcttt taaatatgcc
     3661 ttccgcatat tataatcttg ttcttcctct atcttccgta actcttctta acgattgttg
     3721 tctagcattt tcacaaaaag taatgtattc gtattttcat ttttcatatt tattcctcct
     3781 atatttattc ctgcataaag aaatactcct atcaatcaat gtgattaata ggagtattta
     3841 tatttattat attagtttta tacacacaat cgctttcata ttgcaagtaa actgtctatt
     3901 tctgcaagtc ttttaagaag cttttcacgc tccactttta agctttccac gtctatatca
     3961 gatacgagtt taacgccctc gtggtctttg attttgctat aaatcgtttc aggaacacct
     4021 tttacacgaa cgattgtgtt cttatcagcc gctattctag gacttttggc agagccaccc
     4081 gaagtggcaa agccaccgtt tataagcatt gcattgtcgg agaaaataac ctctctgtca
     4141 cgataaagtc ttttcagaac aacgattgag ccaactctga tttctccgtc ctcgtaaccc
     4201 tcagtataag tgtcgaggtc aagatctact gtgacagtgc taaccgcacc aagttctccg
     4261 cattcaccat agcattcgat gagtaacgcc ttgacagctt ccttgttctc ctctgggaag
     4321 acccagcaag gggcgttcca cttgccctgt atctgctttg cccctgcgac aaagctcttg
     4381 ttatacggac tgtttacctt gattgtctcg ttttcaactg caactttcat gttttatctc
     4441 ctattatatt aattacttct tattgtcagg tatcttagcc cacattttct ctcgataagc
     4501 caactcttgg ctataggttt tatgccattg cttatccagt tcttttcgtt cctcaagcgt
     4561 aagacttcta ccctcatcaa tagccttata aaaggcgtca tcataaatct tttgagcttt
     4621 gtcaaaagct ccaattggat tgtattttct gttaatttct ctccgcttat tttcattgtg
     4681 attcacacac aaatagataa taagcaaaat aattgttgct agtagcattg tttattcctc
     4741 ctcgtttagt tcatgctcgt tgtaaatttc ttcattattt ctgatattgt atacaatatc
     4801 ctcatcggga tatgcttctt taaaataaca ctgtaagtca tcgggtgttg tagctatata
     4861 gattggttca taactgccct caagcttact gcctttgata gtttttccgt caactttgaa
     4921 ctcagcaaga gataagctaa tctcacgctc cagcggtgcg gtttcaatgc cgtctccgtt
     4981 aattaggttt tcgatagtct cgcccttgtc ctcgtctatc ttttcacatt cagcaacgaa
     5041 atagacttca catctaaaaa aacgccttgt tgaaaacact gtaaaattag ttatgtttat
     5101 aatatctgaa tgatatttct tcaattctgc taaagtttcc acctcgctat catagatctt
     5161 aatagggttt cctatgtttt cacacaaaga aactatatca aacaatctct cagggagctt
     5221 ccttaactct actcttgact cgaaaattcc atatttcata caaatttctc ctttgtttaa
     5281 ttaattatac tacaaaattc ctcattagtc aactagaatt ttgtcgaaaa cgtccataaa
     5341 atcggacagt atggctattt ttattaacca cgttttgcac tcattatcag tatagccgtt
     5401 acacttcatt tgtgcgatat gtaatctaat acgctcattc cgttccaatg atctgatacg
     5461 ttccataaga cgcttatcag ggtgctgtat taccatgtta ttctgctttt ctatcataat
     5521 taattcctcc aaaaaatata tttagttgta cttctttgct agttcttcaa ggtcattctg
     5581 tatatcacaa ttcttagcat atacacaact tagaacgtcc ataacctttg caacactgtt
     5641 ggaaagttca tcattattat atttatttac aactttcagc aattcatcaa aagcgtgttt
     5701 aacgtctaaa tctaggtcat taccataaac atattcatct tcctttatgt caagaccatt
     5761 caaatagtca tttagttcac ggaactgttt aaactcagaa gaagcaagtt tagcttcata
     5821 gtctttaact cgattacaaa gataagatgg atttacaaat gcaaatatgt gattatcgtc
     5881 cagtttatac ttagcaagtt caccgaacaa ctcataagat ttattaagta gtttgccact
     5941 tgctacatac acatcagctc cagaacgcaa attggcaaat ttgtgtatct ctttcataaa
     6001 cttaggcaaa tcatcttctg aattaagttc catatataca gatgcttcac tatacataag
     6061 accgaactta caatctatat agtccatatc tttttcatca tcaagtttta ttatattata
     6121 catatttatt acctctagct ttcttaatta tatttatcta cctcagccat tacttccttg
     6181 ataaagttag taataatttg cttcttacgt tcttcattct tataataaaa gttatctgtg
     6241 acatatgtag taacaagagt tacaagtttc tctttagaac tgaatgtgtc tataaaatca
     6301 caatgggtat gaggaatatt gtacatatct tcctctgtat aaacatagtt actaggatac
     6361 aacttatcat tgatttcttc tgtatccaaa catataatca aattcttatc aacagggctt
     6421 gtataatgtt cataaagctt aaatgttttc atggttaaaa cctccaattt atcacctttt
     6481 atagctatct acgttccaaa cattcaaaca gataacgccc ttattatcag cataaacgtt
     6541 atcaatgctc gatacttccg cataattcat atgctccgga acatctccgt aatctccgtc
     6601 ataaacaatt ttctcctcag cgtccgacca tatctgaatg tgttgtgcat caggatcaat
     6661 gaacatttcc ataaattctt gtactgtcat agttaaataa cctcctcaaa aaaattccgt
     6721 ttccgtatgt attcttacat ggtactcttt acaagtatct cccttgctcc gagtatataa
     6781 gaggggaata attcccctca gaatattaaa tcaatccatt ttctttgaac tctcttatca
     6841 gtccgtattg tgtgccaagc tttttaagtt taatttgtat ttcagctaat tccgcatagc
     6901 ttattgagct ttctgacaaa tcagcttgta attgtattgc aagttctctt gttcttgctt
     6961 ttcctcttgt gtatttatta atattcatga ttagactatc tcctatttaa tagtattcat
     7021 tgagatactt gctttcaagt tttttaactt tttccgttat actgctttta tccttgcaaa
     7081 tgtctataaa gtcagcagta ttacacatat ccaagagttt ataatacttt tcttcaacag
     7141 tatatttatt ccaaaagatt aaatattcct ctctctgttg ctttacggct tgttcaatcc
     7201 ttttcatatc ctcacatgga atgtagtctg tagatacgta cagtttgatt attccatttg
     7261 cgcttatatg tgctatctgt ttataatcgt tatgttccat tacagcttta ttacatactg
     7321 taatgccgtt tcctaaacag cacataaata attcaaagtt ccgtttactc atatagttaa
     7381 gcctcctcat cgttaagacc atcatcaata agatcatcta tttccaactc gtaacatagg
     7441 tcattcaaga ccgcttcttg agcagctaca taacgataaa cttcacgctt tttagtgttc
     7501 tttttatcgt tattatattc cctgtctgcc tgctcaagtg cttccgctgt ctcattgtac
     7561 attcttatga tagtcttaat catttcttct cttgtcatgg ttaattcctc cttaattatc
     7621 tctcaattag tctgattata tcatcgccat atnnnnnnnn nnatatactt ttcattttgc
     7681 aactgtttaa taaactccgt gataggcatt gtatatcctc tgttgatttt ccagctatac
     7741 atatttataa gatcttgtaa aaggtcatac aaggtatagc ctataaaaga gcatccttgc
     7801 ggtgaataac tccgcaagtc ccttataaca ttatcgctca tacttatgcg tgttgctccg
     7861 ttgagtttat gtgttattgc gttcatgctt atttcactcc ttaaaacaga tattttattt
     7921 ccaccacgtt gacattccgt catttagtct taccggcata agtaacataa gctgactatt
     7981 atatttattt tcctcaacat ttttgaagat tataggtttc aatgccccac tatggagcat
     8041 tttaattctg tcgccctcaa gatttttaac agcatccgta aagcgtgtga gatcatagcc
     8101 gctggtttcc gaaatattca agccctcaat atcaacgttt ccataaggtg agacaagtcc
     8161 attattcttg attgcaaata tatccatagt cttattagtt ttaatttctt taaggtattt
     8221 caagttttca agcatattct ttttctcaaa ttcaaattca gagctaaagg tacatggaat
     8281 aaatgcctcc cattgaaaat actgtccttt aagattcctg ctcaagagta taaaatcttc
     8341 cgatacaaga ttaaatgctg ttatatcctt aaacaatgca atatcacatt cacccttttt
     8401 gaactgcttc aagattgaaa acgtattatt attcattgta aactcatttt caaagctcaa
     8461 accactatct tccgtatcag tgcttactgc taccctacag ccgtccagag ctaccatttt
     8521 attttccttg aaattaatac cctttaatat aggcttgaga tcgtcttttg tgtatatagc
     8581 atagcttatt gagttataac gctccataag cttctcaatc gtgtatgtat gctgctcaag
     8641 aatgtttgaa ttatttgaat taatattatc aatccaaact tttccaagat gtgtaaaaag
     8701 agaatatgta tcattatcat ttacatcagt tattccagct ttaaaggact ttttgccgtc
     8761 ctcaaagttg cacgctttat cactatcaaa cgtgatagtt gtatcgctgc ccttgaaata
     8821 tttaagagcc tttataactc tttttacgtc ctcaagagca aacatgatct tgtcatcggt
     8881 tatacagttt atagtcttgc agccgataat ctcaagatta tttgcgaaaa tcttcatttt
     8941 cccgtcctca gcctggataa acgcattccg caaaagatag ttagatgatt ttgtatcgat
     9001 gatcttctcc acctgctcga gggctgttgc aaagtccttt gtgcttgtga tgatttttgt
     9061 agtttccatg atttttacct cctctaaaga ttataacaaa ttaatagtta cccaacgttt
     9121 tgcacttgct aaagttttgc aagtttttaa aactgtgtga ccatcgcagt ctattacatc
     9181 aaccatacca tcatactgta attccaacca ataatattta ttttctcctg ccttataaat
     9241 atatacgcta ggatttccgt tatcatcatc acagtcatgc agtatttccc atttgttttt
     9301 catttaattt atcctccgta ttttaaaata aatgttttat tcgcttttca agcgtgtata
     9361 tggcactctc aacgacttca tgcggtcatt ttaagtatat ggggcggtta tataagccgc
     9421 cctcagatca taagattata taggttttaa gcgttataat attctatctg cttgtgtaat
     9481 tcctcttgca tgatctttat ttgattttcc gttaaatcct tgatactcat atttaaagcc
     9541 gctaacgcag gctcataatc acaagtaata caagcctcat gattggctaa ttcatagatt
     9601 atcatttctc taaatacttc atcatttgac ttgatcttgt taaattcggt ttgtaaatgc
     9661 tcaaagattt tatttgcacg cttcacaatt tcattgttct ttacatagca gaagcaagcc
     9721 gggcaaaagt atttatatat tttgttcttt tcctcaaatt cctttttcca ctgttcacca
     9781 attccaaagc aagataaaca atcaaattgc atgatccaat aatttcgcaa gtagtacgat
     9841 tttgcggtgt agtcgtcata tgactttaca acgctcatca actcggcttc agtaaagagc
     9901 tttttgctca attccttgca atagtgatcc tttaaagctg attttccttg ctccttaact
     9961 aaatatttat ggtgtagctc atattcgtta gcgtagtaaa tatgtttttc gttcttgaaa
    10021 acaagtgcag agtatccaaa gtagccgcca aaatcaacaa aannnnnnnn nntatcatga
    10081 tcctttatgt tgatatagtc aagagctatt tcagcagcct catcaaatgt caataactct
    10141 atatcattaa ttgcaagggc ttttgtgttt gtcatgttct tcataaaaaa tatcctcctc
    10201 tcgtgtgcta gaactccatg aaaatagatc tattattatc cttatttcca ttagccttta
    10261 taatgccatt gaccgcttga acaactccat atagccagcc gtttaaatag tctatgtttt
    10321 cgcaaaactg cacataagag ccgtttttag cgtcctcagc agacttgaaa atataccagc
    10381 ctttaccgta gtcagccttt tcaatgtgga tatagcccat attgacgaaa taagccctta
    10441 aattatcctt gataattgca aattgctttg gcgtgaaaac cttcttaatg tcctttgtac
    10501 tgatgccaat agtgtttatt atgttctcca tggttaattc ctcctcaaag tcttaaagtt
    10561 gtatgttgta taacgtgtaa ataatggtta tggtatccgc tccacctcat gcggtttcgt
    10621 ggatataagg ggttaaaccc ctttaaaatg gttcaatctc aattctgctt aatatttctt
    10681 gagccttgct tagaaattcc gcctcatatt catcaacata gnnnnnnnnn nccgtcacct
    10741 tgttcgcaag tttatcgtca ctcttgatat agtcaattat attacgacga tcaacgggga
    10801 aattaatccc aaaatctttg aattgtctaa ccgcctttat aagatcgttt tcggaaattt
    10861 caagagcatc cgcaaattct gaaatgtcat tgaaataatc gtaaacgtca tcataattcg
    10921 gtgaatcact gtagatagta acgtgatcgt cattgagcca tgaatgaccg cccacattta
    10981 taaacgtttc aaggcgtcct atttttgcat ctgcatcata gtaaaaatat acgtccgttt
    11041 ggtatgggtt caagtcgatc tcaaatttcc gcaagatcac cgcaagttca gatacaagat
    11101 cgttgatatt gatgacgtcc tcatcggtga tatagtgctg aatgtttgta ctcattttga
    11161 tacactcctt ttaatttaat gttatattat gtatggtatc cctttaggct catgcgcctt
    11221 taggggatat atagggcgta taaacgcccc ttgaaattaa tttagctcat actcgatcgc
    11281 ttcaatcgtt ttggatctca tttctgtaaa gctatttgat acgggattaa aatagtcgcc
    11341 tagaatacca taagagcagc agggcgcaaa aaatatgtta ttgctattgc tgctgcaatg
    11401 tgtatcacag tatttgcaat tcatgcaatc accatcacat ttttttaggt gattcagagc
    11461 cgcttcaagt tttttacgtg ttgatatagt cataaaatca cccccttaaa cgtgcaaata
    11521 cgcttctttt tgtgccattt ttataacgct attagcagcg tttaaaacgc tttgagggat
    11581 tctatagccg caaataacgt atttgtcaag gcgtgtattg tagccgattg aatagttcca
    11641 gccgtaaaca ccagcattat aatagttcgt gctatcagta taggcatcaa tgccgctatc
    11701 atcaacaaga ataacatctt gaccgctgat tatgttttta gcgtcgttgt ttgtgatttt
    11761 ttttgatgtt gtgtacataa aaatacctcc gtttttttcg atttacgttt gttttgtctg
    11821 ttttgttttg tgttgtctca tttcttttgt tttactgttc tacacttgac tttttatttc
    11881 ttttgtgtta tcctaaaaat atggttgatt aaattcagat cattgcactg gattcaatcg
    11941 tatagcggtt aaaataaccg ctaaaatttt agcagatgcc ctttttttat atcccagaaa
    12001 ttcggaaaac tggattaaa
//
LOCUS       DAWEMR010000071        11961 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2264_length_11961_cov_11.544767,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000071 DAWEMR010000000
VERSION     DAWEMR010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11961
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2264_length_11961_cov_11.544767"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3339 a         2811 c         2383 g         3428 t
ORIGIN      
        1 tcggatatgc acattggaca ccaaaacgtc ctgcaccatg acaatagact ttttactgac
       61 atcaaccata tgcaggctgt catgacggca aattggaaca gcaccgtttc cgatagcgac
      121 gaagtgtata tcctcggtga ctttgcatgg aaaaacactc aggggcttga aatgctctct
      181 cagcttaggg gcaaaaagta tcttatcctc ggcaaccacg ataagcccac agaagaaatg
      241 aaagcctgct ttcagtgggt aaaagactat gcagtgataa aagacgacga aacgcagatg
      301 gtgctttctc attatcctat cgcccattgg tataatcaat atcgtggggc tgttcacctc
      361 tatggtcatg tccataacac caaggactat caggcgtttt tgcagtatgc acaaatatgc
      421 aaaaaccttg ccataccatt tgaaagctat aacgtgggct gtatgctcta ttatatggac
      481 tacaccccca gaacacttga tgaaataagg aggagcgcaa aatgatactt ataacaggcg
      541 atatccacgg aagccacgat atccataagc tcagcaacaa aggcaacccc gacttcaagg
      601 atattttcaa cagcatggca aaggatgatt acgttataat ttgcggagat ttcggacttg
      661 tatggaacaa tgataaggaa gatatgtggt ggagaagctg gcttaacgac agacctttca
      721 ccaccctctt tgttgacggc aatcacgaga atttcgacct cctgaacgct tatcccgtag
      781 aaaactggca tggaggaaaa atacaccgaa tagccccaag cattatccac cttatgcgag
      841 gacagctttt cgatatcgag ggcaaaagct ttttcaccat gggcggtgca gaaagccatg
      901 acagagaact tcgcaccata ggcataagca tatgggagca ggagcttcct aataacaaag
      961 aatacgccca agcactttcc acccttgaaa agtgcggata caaagccgac tatgttataa
     1021 ctcactgcgc ccctacagat atcgaatatg aggtggagca tggaaaatgc acgtccgctt
     1081 ataatttcta ccattcagaa aacaggctca cagagttttt tcgtgacctt gcagagaaaa
     1141 tgcaatatga acgctggttt tgcgggcatt atcactgcga ctatgtgagc aaactaaacc
     1201 cgaagtttga gatactttat gataaaatag tcacactgcc gtaatagtct gacctatgag
     1261 cttgtaaaca acgcacccct ttgacttccc acccccgcca tgctataatg ataaaaaaga
     1321 ggtgacccca catgaccaat aaagcatact accacctgcc ggggcttttc gagttttatg
     1381 acctttacgc cgctttcctt cccatattcc gtgagcatag agaatatttc tatgactggt
     1441 gcgaaatagg ctctgtctat ggcgcacctg ccgactgtat atggggcggc ggacgagcag
     1501 gcttcggcga acattctcca cgagaagtcc tcgaccttat gaaagaatat aacatctcct
     1561 cacggctgac cttcagcaat tccctgctaa gagaagaaca cctttcagac aaaaagtgca
     1621 acgacctttg caggctgttt tcccaaagcc cgcctccaca aaatggtgtg ataatatgct
     1681 ccgacctgct tctttcatat ctgaaagaga attatcccca cctctatttt gtgtcctcta
     1741 ccacaaaagt cctcaccgag ttttctcagc ttgtaaacga gcttgataat gacgattttt
     1801 cctatgtagt ccccgacttt cgcctaaaca aagccctgga taaactctcc gatctcaccg
     1861 ctgagcagaa aaacaaagtg gagtttcttt gcaacgagtg ctgttatata ggctgtgcag
     1921 accgaaaaaa ctgctatgaa aacgtcagcc gcaaaaatct cggtgaaaac tgccccgacc
     1981 atgtttgcaa agcacccaat gcctcagagg gctaccgctt ttcaaaagcc atggaaagcc
     2041 ccgcctttat cggcatagag gatatccaaa acacctatct cccaatgggc ttttcaaact
     2101 tcaaaataga gggcagaagc cttggaagcg ccctgatact tgaatttctg ctctactata
     2161 tgacaagacc cgaacatcag ctaaaggtca gagagcaaat ttatcttgac agtatgctgg
     2221 atctgttctg actattcttc acatcgcata tctcctatgt cgcaagaaat atgtcgcaaa
     2281 actcatgttg cccatggctt acttggaaac aaaaatcaaa ataagtgtaa acaatgccac
     2341 tttttttgtt gaaacagcca ttataatata gtataataac acagtgcttt gtcacaaaaa
     2401 agttatagga gaatttacat gaagaaaatt atcgacatat ttctgcgttc tttgctcaca
     2461 ggcgtggcga tagccatagg cggaacagtc tatctttcct gccctaacaa atatttggga
     2521 gcgcttcttt tcggcatagg acttttcgtg atactgtctt tcggctttaa tctcttcaca
     2581 ggaaaagtcg gctacgcagt tgaaaacaaa ccctcttatc tcggtgagct tggcataata
     2641 tggatcggca attttgcagg agccgtcctc tccgtcctgc ttattaccca aacacgcata
     2701 tccatcataa gcgacaaagc cacagagctt tgcaatataa agcttgggga taatatcaca
     2761 agcgttttta tcctatcatt tttctgcgga atacttatgt tcgttgccgc agacggctat
     2821 aaaactatcc aaaaccctgt gggacagata cttgcaatat ttctccccgt tgtggtcttt
     2881 atcctcagcg gctttgaaca ctgcgtcgct aatatgtatt tctttaccat agcagattta
     2941 tggagcatta aagcctttgg ctaccttatt ataatgtccc ttggcaactc cattggcgga
     3001 atacttatcc cacttctgcg aaaaggcttt gtatcaaaag catgacgcca atttttataa
     3061 gcttatgtaa cacataaaaa aagataccgc agttcaaaaa gaactgcggt aaaaaaatga
     3121 cttctgctct caggaacaca gtaaaggggg tattatgaaa aaaacaaata aaacaaagga
     3181 gtcaatagta aacataagat aacaaataaa acacaattta aacaacttag aaggagtgtg
     3241 cagaagtctt tgttcgttcc tgatgtatgt attatatcac actaaaaacc atttgtggta
     3301 actttttttg gacagttgcg acttttttca aaaaaatctg cactccgaaa atcctaaagc
     3361 tttcagagtg cagatttatc tttttttgta gaggcgaaca gcgtttgccc cttcccacgt
     3421 ctatccgaat tgtcgcctgt ctatcaaata atatccgccc tgcgtttacc tatctgtgca
     3481 aactctttgc gccttgtgat aaagtaagcc actgttaccg cagtgcctgt tatcacccag
     3541 gaaacagggt aaactatcat aagcataaga tagcttctgt gagccgcata aatcgtgttc
     3601 acccacacaa gcctgaacac gcaggtgcca agcacagtta tgaccgcagg tgtcatagag
     3661 tgacccattc ccctaagaca gccgcccgat atctcgtaaa cacccgtaag aagctccagc
     3721 agacaaactg accacattct tatcattgcg tagtgtataa cgttctcgtc cgttgagaat
     3781 atctgtataa aaaggtgtct gccaataagg aatatcagac tcagcccaag acagcttgca
     3841 agcccgataa aggtgcaaag cctgtatatc tttttacagc gttccttatc ctgagccgca
     3901 aaattctgac ttgtaaaggt ggtcgcagtc tgagcgaaag cgtttatcac aaaatatgcc
     3961 atgaactcaa aattctgttc cgctgtgctt ccggcgatac cgtctgaacc gaagctgttt
     4021 atcgccgcct gtatcacaac gtttgacagt gagaaaacca tgctctgcaa accagccggc
     4081 aagcctatct ttatgatacc gaggagatat ttccagcgta tcctgagctt tctgaaatca
     4141 agtcttagca tttcctcctc gtgggtaaga aagtaaagta tcatgcaggc actgaaaaag
     4201 tttgatataa cagttgccgt tgcaacgcct gccacgccca ttttgaacac gatcacgaac
     4261 acaaggttca gaataacatt tataacgcct gcggtgataa gagcgtaaag aggtctggtg
     4321 ctgtcgccct tgcttctaag caccgccgaa ccaaagtcat atatctcgat aaaaggcatt
     4381 gcaaagaaga atatcctgag atacagcgtt gcaagcttta aaacatcgtc aggcacattc
     4441 atcatgccaa ggagtatcgg tgcaagtatc tcgcctacac ccagtatgat aaatccgctt
     4501 atgaccgcca aaagcaccac cgtatgtaca gcctcgtttg ccttctcttt tctcccctga
     4561 cctataagtc tggccaccac aacgtttgca ccgacagaaa gccccagaaa aaggcttaca
     4621 ataaggttca ccaatgaaga attgcttcca accgccgcca tggctttgtc gctgtcaaat
     4681 ctgcccacca ccgcaaggtc agcggagtta aaaagctgct gcaaaatact tgacaatgca
     4741 aggggcagtg caaaaataac gatcttgccc acaagcgggc cgtttaacat atctattttc
     4801 ttatttttca taccagatct tctctcatct ttcaactaac tatttctcat acctaatatt
     4861 atatcacacc atcaaagcta tttcaatact cgaatttgca cataagcaca cacagagatg
     4921 tgtaaaaatc taaacattac gaccatttgt acaatttttt gaactctttg cctgttgaca
     4981 aaggcacgaa atggtgttat aataatattc cacgggaggt gagcagtatg gcgatagaca
     5041 aaaaaggcac aaagcagatt gccgacaacc gaaaggcacg tcacgattat ttcgtgcttg
     5101 agcagataga gtgtggtatc gagcttgtgg gaacagaggt caagtctata aggaatggcg
     5161 gcgtcaatct gaaagacagt tggtgttcta tcgaggacgg agagatgtgg gtcaagggta
     5221 tgcacatatc cccttatgaa cacggcaata ttttcaaccg tgatccacgc cgggagagaa
     5281 agctgctgct ccacaaacgt gagatactga ggctgttcgg aaaggtacag caggagggtc
     5341 ttacgcttat cccgatgtcg ctatatttca aaggcagcag agtcaagctt gcacttgggc
     5401 tttgcaaagg taaaaagctt tatgacaagc gtgacgacat ggcaaagaaa gccgcacagc
     5461 gtgacataga gcgtgccatg aaagagcgaa actaacctgg gggtgtaaag gtttcgacgg
     5521 gggttttgaa gcacgataag cgagtagagg agcgcacact ctttaaacgg cgtacgtttt
     5581 aaaattagac gacaacgata attttgaatt agctgctgct taagtcagcg ctcgtcttgc
     5641 cttagagtac cacggctttg gtaaaggcgt cgatcagtgg tgaacgttac aaagcgatgg
     5701 ctgtagcctt gtgatgtatc acagcctacc aaacacacag gcgtgtctgt ttgcctttgt
     5761 gcaagggaac gggagttttc cgcaataaca gaatacactc gtagaagttc gtggggatag
     5821 gctttcggac aggagttcga ttctcctcac ctccaccagc aggggcgtgc ccctgcaccc
     5881 aatccgcttt actttatgta aggcggattt tttattgccg ctcatcaatt ttatctaaaa
     5941 cccaaccatt gtttcgccgt ggcgggcatt tcccatcaag tgtttttgta aacacacaaa
     6001 gtcaccgctg tgataatttg tgtgtcactt ttacaaattc aggggcgtgc ccctgcaccc
     6061 gatccgcttt actttatgta aggcggtttt tttattgccg ctcatggatc attatttata
     6121 aacccaactt tgttatttca ccatggcaga cgctgatata aaaaaagagt gcattaccac
     6181 ctctgtgaaa tgcactctta tattttaccc tatttttctt caagtccgct gagcgaaagc
     6241 ctgctctcca ttttctgaac agtcttgctc tcctgctttg cgatctttgc ttcaaaccag
     6301 aatgtcgagc ctttgccctc ctccgattga acgccgtatg caaagccgtg ggctttgagt
     6361 atctcctgac aaatggaaag tccaagtcct gtgcctacca cgtcacgctt tacctttgcg
     6421 tcacggtagt atctgtcaaa tatcttcggc agaagctcct tagaaatgcc ctttccgttg
     6481 tctgttactt caatacgaac cacatcagcc ttgtttatct gcttgatacg aatgacctta
     6541 ttgtcacccg tgtagtttat agcattgttt ataaagttgt aaattacctg atccagcttt
     6601 gcaatatccg ccgttatcac tctgtcctcg tctgggatat acttgatatc ataaccattt
     6661 tgctctatga gtatctgata tcgagtcatg cagtcattga gcttgtcaac tatagagaag
     6721 tccgtgagtt tcagctccgc attgcccgac tccagctttg aaagctccag cagattattt
     6781 accagacttg aaagcctgtc agcctcgtcg ataatgactt ggatatgctc ctgcctcttc
     6841 acaggattat cccccgacaa gtcccttatc atttccgcat aagccttcac catagtgagc
     6901 ggtgttctca ggtcgtgaga tatgttcgca ataaggtctt tcctaagctc cgataccttt
     6961 tgtatctcgt ccttggcatt gttaagcacc gtggaaagct cctctatctc acaatagcca
     7021 tgaccattga actccaccgt ataatcccct tcaccgaatt tcttcgccgt gttcgtaatg
     7081 tcaacgatag gctttgaaag tcgctttgag atatacatag ttacaataaa agcaagctca
     7141 aaaagtatga tagtgatata aatgagctgt tctcttataa tgcttacagt ggaatctata
     7201 ggctctattg agctttcaat gaaaagatac gcctgcttat ccgaatcatc agtgtagata
     7261 gacgtgcagt aaaatatctc cttgctcttg aaattctcat tctgagcaat tttcgtgatg
     7321 tagttatcgt ccgactgctt tagcttagat atcatcttga aaagtccgat gccatagccc
     7381 ttgtttatga tcttgttaag atgcgaatat gctcccatgt tgttttccat cacaatagga
     7441 ttgcccattt cgtccgttat aacaatgcaa aggctgttgt caaaagccag cgagcggttc
     7501 gttacctcct gctcgtcagt gccatatgct tggattatct gatttgcagc gtctgtaata
     7561 tctcttatct tcgccgactg ataatatctt tccaagaaaa aatactgaaa aagccaaata
     7621 agaataagaa taaaagccgt gaagaagata aaatacatcc acacatatga ccgcagactt
     7681 cttttcgcct ttatctccgc cgtcttattg ctgacttttt ccttgacttc tgcaagctta
     7741 ctggatcttt tcaaacttat atcccattcc tctcagagta acaataagat tgcggtattt
     7801 tccaaggctg tttctcagca tttttatgtg agtgtccaca gttctgtcgt caccgtaaaa
     7861 atcaaagccc catacctctt caagaagttt ctctcttgaa agcgcaatgt tcatgttctt
     7921 caccagatag aaaagcaggt catactcttt cggagtcatg gaaaccttct caccgtctat
     7981 ctttacctca cgggctgtaa agtttatctc taatccctcg tatcttataa cgtcctcagt
     8041 gcgctgagaa gctgacgcgc ggttaagcac cgcccttatc cttgccataa gctcctttgg
     8101 cgagaacggt tttactacat aatcgtcaac gcctatctca aagccgaaaa gcttgtcata
     8161 ctcctcgccc cttgcggaaa gcattataac aggtatctgc tttatttttc ttatctcctt
     8221 gcaagctgaa tagccgtcaa gcttcggcat cataacgtcc attatgatga tatcgaaatc
     8281 ctgatttttc accatttcca cagcctgcat accgtcctca gcctgagcta cttcatatcc
     8341 ctcaaactcg gcatactctt tgattatctc tcttatttta atttcgtcat cgactacaag
     8401 aatttttgcc ataagtattc ctccctattc aggcaacacc gcacaagaac cgcctattgg
     8461 ctttgcacgc aatctgcttg catacaaatc ttgtgtgttg ctaccttaat tcatattctt
     8521 tgcttatata ataacctatt attgtgtaaa atttgtgacg gtttatagaa tattctaagc
     8581 cataccgcac caattcacaa gaaaactact gcccgccaac caaaatggta tgacgggcag
     8641 agcttatata ggaatttgtt atggaaatgt caggaaatca gcaatgtccc atcataatga
     8701 gagccataag acaaaaacct gttacgatac aactcattgc gcccatgatg ttgtcaattt
     8761 tttctgctgt aagctttttc atagtgatta cctccttggg gtctaaaata aactattctg
     8821 ttctcagtgc gataacaggg tctttctttg ccgccactct tgacgggaac agacctgcga
     8881 taagcgtcag gcaaacgctt attgcaatga gtataactgc cgcactgaca ggtattgaag
     8941 ctcttatcgg cacgtctgta agatgtgcga ttatcatgtt tatcggtatt gtgagcagat
     9001 atgagatcat tattcccaat gcacctgcca caaatcccac gataactgtt tcggcattga
     9061 acactcttga tatatccctc tttgaagcac ctatacttct cagaatacct atctcctttg
     9121 ttctttccaa aactgagatg taggttataa tgcctatcat gatagatgat accacaagtg
     9181 aaattgcaac aaatgcaata agaacgtagc cgatagcgtt tatgatagag gtgatagaac
     9241 tcatcataag accaacataa tcggtgtatt ctatcttgtc ctcttcgccg acactgtcgt
     9301 tgtaatcgtc gataatatct attatctttt cctttgaagc aaagtccaca ggatagatat
     9361 ttatgctgtc agggtcttca acaactgaat aaccaagagt tttcatgttg ttgtcatagt
     9421 ttgaagtgga atattttccg ctgtttgcaa gctgatccgc atttgtgtcg gactgttctt
     9481 tgagcatttg cttcataagc tccagttgct cgtcagaaag tgaagctata tacttcttct
     9541 gagtttcggt aagcgatgac atatcaccgc cctgaattgc tgacatatcc attgaaccca
     9601 tggcattcat gcccatatct gcaagctcgc ctgtatcgga agcaccgctc tgctccataa
     9661 gagccttata ctgctcctcg gtcattccct caggtatctc catagagctg tcgcctgcca
     9721 ccaaaccagc cttcttatct gtgtcggagc tgtctgctgt tttgtcagcc ttgctttctg
     9781 tatcggctgt gctgtcggaa acgatttctg aactgctgtc tgtatctgcc gcattctcgt
     9841 ccgttttgaa cttaagtcct gtgaaaacat ctatgtcagg atttgctatc tgctctttta
     9901 ctatctcgct gttatctact tcgtcgataa gatatgtcat aaggtcatga cgatagccta
     9961 tcgcaccgct tatggaagca gaggaagcgt cctcgtcagg tcgcaggata ccgactacac
    10021 ttatttctgt gccgtttgca acagcctttt tcatgtagct ctcatcgcct gtcatatctt
    10081 cccacacacc gtcttttttc ttataaaaat caggtgtagg aacgaccttg aatttcaggt
    10141 caagaagctc atcataggtg taaacgtgct gttcgctgtc aacggatata tcctctcctg
    10201 ccatggcacg tcttactatg ccgttaagtg agcttgggtc gagaagtccg agagaataaa
    10261 gcacatagtc gtttatctcg ttgttcttat caactacaag cacaacttcg ttatactttt
    10321 caggcaaacg tcctgcgata acgtcatact gcttgtcgat aagctcctca ttatctatca
    10381 tttcgctcca tacgtcagta cccatgccca tcatgccacc cattgagcca ctcatggaca
    10441 ttgcttccat ctgagttgag gacatatatc ccatatcttc aagaagcgtt gacggattga
    10501 cctgcattac accgctgtcg ctctccgtaa aaacgttaag tgtagtggcg tatgtgtact
    10561 gaacgtcgct tgtaaggtct ttcaggtcgc tgttatcgtc aagatatttc ttgaacttct
    10621 ccagattgtt gacctttgtt tcttccatga gagtgtttat catctcgccc atggcattct
    10681 gagaatagac cttgtcttta tcgtggtttg ctgaatcctc atggtttttc gccatggtgt
    10741 tcattatctc gcctatactt gcggtagtct ttgttatctg aagcggatag cttgaaagtg
    10801 tatcagactg cacctgatct atataaagct gaacgccgtt tgagatagaa agaataagtg
    10861 cgataccgat tataccaata gagcctgcaa aagaggtaag aagcgttctt gccttttttg
    10921 tcataagatt gtttcggctc aaagacagtg cagtaaggaa gctcatggaa gttttaagct
    10981 ttttgcgctc tttcttcgcc gcctttttgt cagcctttgt aggctctttt tcaggctttt
    11041 tgataggctc aacaacattc ttgtcaagag ggtcggaatc gtctatgacc tttccgtcaa
    11101 gaagtttgat tatccttgaa gaatatttca ttgcaagctc aggattgtgc gttaccatta
    11161 taataagctt gtcctttgaa atgctcttca aaagctccat tatctgcacg cttgtttcag
    11221 tatcaagcgc acctgtaggc tcgtctgcaa gcagaatgtc aggctcattc acaagcgctc
    11281 ttgcaattgc aactctctgc atctgaccgc ctgacatctg attaggcttt ttgtggagct
    11341 ggtcgccaag acccacctgc tcgagagcct tgacggctct ttcacgtctt tctgactttg
    11401 aaacacctga tagggtcaaa gcaagctcaa cgtttgaaag cactgtctgg tgcggtatca
    11461 ggttatagct ctggaacacg aagcctatgg aatggtttct gtaggaatcc cagtcgcaat
    11521 ccttgaaatc cttggtagat ttgccgttaa tgttaaggtc accgcttgta tatctgtcaa
    11581 ggccgccgat aatgttcaaa agagtggttt taccacagcc tgacggaccg aggatagaaa
    11641 cgaactcatt ctcacgaaaa tcaatgctaa cgcctttaag ggcagaaacg gtggaatcac
    11701 caacgacata atcttttttt atgtccttta aactaagcat atattttctc ctatttttgt
    11761 tattgtaaag atattataat agggttttat aaactcagtt taaacataac acagttttgg
    11821 gtaaaaaaat tgtcggaggg gagatttaag tgaggagtga gaaatgagga atgagcatag
    11881 caaaccaaag taaattgcaa gcatagagta aaataaagca ccgagcgaag cgagagataa
    11941 agcgcacgct caagcccggc g
//
LOCUS       DAWEMR010000072        11957 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2266_length_11957_cov_9.852378, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000072 DAWEMR010000000
VERSION     DAWEMR010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11957)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11957
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2266_length_11957_cov_9.852378"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1835..1844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4110..4119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11072..11081
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3469 a         2821 c         2388 g         3249 t
ORIGIN      
        1 attagcaatg caaagtaatc caatcacaat aggttcagat atagatataa tcagtaaaaa
       61 caatatgttc caaaatattt tatgacggat tgaatcatca taacaaataa aggacatgga
      121 gctaataaga ataatggttg taatcagata cactaaaata ttatctgtca acaaaactgt
      181 cgctgacagt attacagtag aaagaagata cgacagataa agaactgctt tattaacttt
      241 tgccttactc aacaaggaaa agtataagaa taccaacatc atttcaattg ctacattgac
      301 aatctcaatg atattttcaa tcaatacggg caactcgatc atctccctgt tgcatagtta
      361 agatagcgag caactatctc atctttatat gttttgccta tggggatttt tctgccgttg
      421 cttagaataa tatcgtactt ggtaatattc tgtatatggt caagatttac aagtattcca
      481 cggtttataa tagcgaaatt gtactttaac aactcgccac acacagttga aaaatcgccg
      541 ccgtactcca attcaccatt aactgtgaat agtgatacct ttcttctatg tccctcaaag
      601 taaagaatat ctgtcagcct gaacttgaat gacagatttt tattattgaa agtgaaagtt
      661 ctaaatcgct gctggcactc agcaatgata gaatttagct gtttggtata tacatagtct
      721 ggctgatttt tcaatatata gcgaaacgct cccacatcgt agcccttaac ggcaaattcg
      781 gcataattcg tcaggaaaga aataataact gaaccatcta tctgtctgat tctttcagcg
      841 gtctgcaatc cggtcagatc gggcatttca acatcgagta tgatgacttc atacattccc
      901 ttgacataga aactcaataa gtctctcccg cttgtaaatt cgtgtatttc gcagtcgggg
      961 agtttcattt ctcttaatct gttatgaagt tgttttaaga atacaggttc atcatcaacg
     1021 atagcaatat tcatagcaat cacctcacaa ttggtcatgc tttatgtatc agttatcatt
     1081 ataccacatc ggagagatta tttcaagaga aaaaatagct atgcctccat taatagtttc
     1141 ggagacatag cctcatgttg cgttatttta tattcatcga atatggtcta ttgcctgatt
     1201 taagagtggg tgtacttata agtgcttcac agaagacagc gagaaaacgc cggaatacaa
     1261 ggtcacttgg gtcgataagc atgggaatac ccataacggt gtcgtgggcg gcgacccgaa
     1321 atgcgacggt cacaagctcc cgtgcctgtt tgaagcaggc tcttacgaag cgcattatat
     1381 gaccaccggc tccaaggagc gcatcttccc gcataccttt gtgtttgaca gaagctatga
     1441 cgcagtgggc ggcggcagag tcgatgtgct cagatgttcc gtctgcgact atatcgttcc
     1501 tgtgaagagc catgtccatg attacggctg gaactgggac tgcaccggct tcgacagtat
     1561 ctatgagtat accttcaaca tcggcggctc gaccaagacg gtggaggagc tcatcattga
     1621 atctgactat gcccaccccg aaaagtgcaa ggttcccggc tgcgaggaat tcatcatggt
     1681 tccacatagt tggggccgct ggaatgtggt cgaaaacccc gataccacag gcagcaatgg
     1741 cggcatggag gctgaatgca gcatttgcga gtacacaaag acaaaaatcg acaaggatga
     1801 tgacgaagga accgatcatt ggacaaagga caccnnnnnn nnnnaaggac accgcccttg
     1861 tgacggtgaa aaacggtcgg gcaacccgta tgatcgtcaa gcccggcgac aagatcaagc
     1921 tttatcccga ggagagaacc ggtcagaaag ccatcggctg gaaggtggag tatctccgcg
     1981 aatacaacgt gtctgacagc actgcaccgg ttcataaaaa ttggagtgcc aacgaggcga
     2041 agtcccagtt caaactggtg accaacggct ccgcccttga gtggggctgc acgatcccag
     2101 agttcagcgc catgggtgcc cctggcggcg gcagtttctt ctttgagcct gtctatgcag
     2161 gctgtgacca cagcggcggc accacggtgc tcaatgcgaa ggagcagatc tgcgaccgta
     2221 agggctacac cggcgacacc gcctgcgcag actgcggcca tgtcatcagt gccggttcgg
     2281 atatctaccc acctgcaaac gccggtcata ccggtccact gcagccgctg tactactacg
     2341 gcacggccaa ctcggcaacc acagacgcga gccatggcgg caaacgctat aacgccaaat
     2401 cgggcgactg ccgacaccgc gcctatgagg gcgactaccg ctacacggct tgcggcggca
     2461 ccgtaaaggg cgagaccggc gacttcaggc atagcggtcc ccttgaactg cgggatgtaa
     2521 gaacggcgac ctgtacagag aggggctatt ccggcaacca gtactgcaca tcgtgcgaca
     2581 ggatcgcgca taagggcgtc tcaaccccgt ccctccacga aattgccgat aactacaagc
     2641 tgatcaatga ggttaagcca ggatgcacca cctcaggcta tagcggcgac cagcagtgta
     2701 cagccggctg tggacagatt atccgctacg gtcatgtcgt taataagctc agccacgact
     2761 gggacggcgg caaacccgca aaacagggca agagcgaagg aattctctac acctgccagc
     2821 gcgccgactg cggcgagaca aggttcgtaa agaactccgc tgcgaccgga tacattgtca
     2881 cggtcatcaa gggcacggca tcccttgccg caggtacact catcacagac aagatcgcgc
     2941 agaacaccgt tgtgaccgtt acggcggacg caccagaggt aggcaaggtc tttgacaagt
     3001 gggtcgtgct ggagggcaat gtcaccctga cagacgccac caaggcaacc accaccttca
     3061 ccatgcccgg taatgctgtg aagattgagg ccacctataa ggatgcacct ccaagccata
     3121 ctcacagcta cggcaccgag tggaagtatg acgacaccga tcactggcat gagtgcgagt
     3181 gcggcgacaa ggccaatatt gaggctcaca gtgccagcga gtggatcgtt gacacggcag
     3241 ctaccgaaac cgccgacggc actaagcata aggaatgcac cgtctgcaag aaggttcttg
     3301 aaaccgcacc gatccccgca acaggcagca atcataccca cagctacggt acagagtgga
     3361 agtatgacgg caccaaccac tggcatgagt gcgagtgcgg cgacaaggcc gacacggcag
     3421 cacacagctt ccagtgggtg atcgacaagg ccgctaccaa ggaggccacc ggcatcaagc
     3481 acgaggaatg caccgtttgc ggcgccaagc gcagcgagaa tacggtcatc gacaagctcc
     3541 ccgacggcgg caacaccgac aatacaggca gcggtgataa taacaccgat aagccaggaa
     3601 atgacgactc caccaagtgt cctcagaccg gtgacagcaa ccacatcggt tggctggctg
     3661 ctctgtacgt cagcggcggc gttctgaccg tgctgggcgt aagcagcaaa aagagaaaga
     3721 aatccgaggc tgaataagcc gacgcttccg aaatcgaaat aacctaaata caataggcgg
     3781 tgacggatca ttccgccacc gcctattttc ctgaaagcgg gcttggcagc aagtcccttt
     3841 ccgaaaggcg gcaagcgaca cccacattct gaccatgtat ccccagccga acggggacgg
     3901 aaatctccgg cgctctttct ttatgccttc tgcaaaaatc agagcaaagc tcaaaaatct
     3961 tttagaaaat taaaaaactt ggttgtagcc ccctcaactt ttcggcatgg aaagtgaggg
     4021 ggctttctct atagcctccg cccgttcttt gaaaaccgaa tagtacagcg catcctgtac
     4081 cttccttctg ccgtcggcga aaccgataan nnnnnnnnng tgcatccttt tgtagggcgt
     4141 aatttttttg tgcctaaaag caaatttact cactgtcaaa agcttgaact cactacaaat
     4201 aaaaaaatgg taacaccagc aggaagttat tttcctgcta atgttacctc atttacaagt
     4261 atcacaagat ttaaagggga aatgataaag actttgtaat gcttatcgta attcttgtag
     4321 ctttacgctt aattcttgta aatgtttttt ggctctttag cttgatagcc accatacatt
     4381 gatgcacatc cgcaggcagc tatacccatt ctcttagtcg ctctggcaat aaggctggca
     4441 agcaatttct taaatgatct cattcttatc acacctcttt tctttgatct tcgctgatat
     4501 tattaaaact gcaattaata gatttgttaa taagatcata gatccaacca tctgatatct
     4561 attgataatc gccccgccaa acagatcaag caacagaaaa actatcattc ccatcgcttt
     4621 gaactttggc tttttctctt tttctatagg cttatttttg ttctccacag ggcagaatac
     4681 aaaagctatt atctcttcaa acacaaccaa ggcaacgatc acccatatat ttatatatcc
     4741 gcataccttg tcattaagaa aagctgtagc aataaatgtc gaacacatca acagattgca
     4801 tctgaaatat gtatctgcat gataccctcc ggtaataacc ctgacagcaa taaacactgc
     4861 caaatatatt acactttcca gcaaatgccc tgtcaatgct cccataagca atacaagcac
     4921 aatgtttaac attgatgaga tagttatttc tatgccatac cgataaaaat cctgtacctc
     4981 atctgctttg ccgatagctt cctgctcggt caaaaaatta agtatacccg atacaacaag
     5041 atccataaac taccgcctcg cttttgtctt accctaattt gaattttact agaataacag
     5101 caatatgtca agctttttgg aatgagttgc aggtttttgt cacgaaatgc agcaacggtc
     5161 cagaacaatt agttcaggac cgttgtatct gccatcaatg ttacctgaca gcaaaacatt
     5221 ccctccgact catagaaatc gcatctgcca ttatattttc ttgctatctc tctgattatg
     5281 gaggtcccca gaccatgctg ttttttatca gcctttgtgg tcgtaagcct tgggttacta
     5341 tccataacag aatgatctat tgaatttttt atcaaaaacg tatatgtatc cgtttctcgg
     5401 tcgatcttca gatacagaaa tttatttgcg tcgtcggcta tttccttgca tgccgttata
     5461 gcattctcca acgtgttgct caacaggtcg caaaggtcag ttccctttat tccctcaaaa
     5521 tctgaaactg ttatacatga tatttttatc cccaaggaag aagccatcgt aagctttgcg
     5581 ttaacgatag catttacaat attatttttc gttttaacat aggactccga cttcccgata
     5641 aggtcaaagc tttctgagat aaattttctc gtttcatcta ttttttggac tttcaacagt
     5701 tcattaaccg tttcatattt gtcttttata tcatgcctta gttttctcat ggcatcatat
     5761 tgcgactcag cgtttccgat aaactgctca agatactgct ctcttagttt gactatctcc
     5821 atttccttga gcttccgatt cttttttatc aaagaattta ttatccagaa tataagtata
     5881 ttaagaacaa agacaagcat atatgacaga tttatataca gcctatggcc accgtctgcc
     5941 gaaagggaca gttcatgtat taccataacg aggaaaaacg aaataataag cactgcaatt
     6001 atcgtcaccc aatctgaagc cgtataatca tctgtatgtt taaaggccat cagtattaat
     6061 ctaaatacaa tgaatataga gatctggatc attataagcg tcacaaatct aagcaagctt
     6121 tgttcgacca caagctccct gagggatatt ttgaaaaatg acgatacagc atttagttcc
     6181 aatgacgtta tcaacaagat cgcaacctgc gagagggata tggaaagaaa tttctttact
     6241 gcattgtcac taaaaaagca aaacgcaaaa acaagcagac ttgcccagta caaagcactt
     6301 ccaaagccct caaatacagt gaaataatta aaagcagtga ccagacagcc ttcacaaacg
     6361 gcaaatataa cagcccatat cttagccctt tcatgagatc ttggcgtaag aaatttatat
     6421 ataaaccatg tttccagacc gccctgataa aagtttatca agatctcaaa tatcgtccac
     6481 aatgtaatag tcatcacttt ctcctcagat attcagtcaa agcgtcgtca actgcattct
     6541 tatatcctcg gctcatggga agctctgaac cgcttttaaa gatcaccgtt tcatttgaac
     6601 ggcttatgtt aaaaacatat ttcagattga cgatatattt tttatgcacc cgtataaaat
     6661 caaatttacg ataagcttct tctatctcgc caatgctatc tctgactgca atggtatcct
     6721 tattatttgc aaaatgatat atgatgctgt ggctattgct ctccatataa gttacatccc
     6781 gaagctttac agagatcctt ccctgctccc tattctccaa aaccactgtc ttttcctgct
     6841 tcagtgcgtc cgtcaactgt cccgctacca tatggagcct atccttcata acatcagcgt
     6901 caggatcttt gcagataaaa ttcagcggtc tgaaattaaa gctgtcataa accagctcct
     6961 gatgacttgt cacaaagatc acgaagcaat taccattctg acctagctgc cgcacgactt
     7021 caaaaccggt catttcaggc atatcaaggt caagaaatat agccgaaaaa ggctttttac
     7081 catggcagta aaaaaagctc tcagctttgg taaacgtttc tatttcagcc ttgaccgacc
     7141 ttgattcaag ttcatttgcc agcatagatt taaaaaactg aagaaaaaca gcattatcat
     7201 cacaaactgc gatccgcatt ttttcacgtc cttttataac atagtctatg tacgatttta
     7261 tattaccata attataccat aaggtcaagc cataaggcgt ttcacagctc cctgcgtcaa
     7321 gaccacacct ggcggcaaac aacaaaaggg ctgtccacac agcccttttg ttcttatcta
     7381 acctatgcta agccccttac accttaggct ttttcatagt aagcgaaata cgctttttct
     7441 tcaggtcaac gttaagtacc caaaccttaa ctacctcccc gacctttacg acctcaagag
     7501 ggtgctttat atatctgtcg cacatctgag aaatgtgtac aagtccgtct tcgtgaacac
     7561 cgatatcaac gaacgctcca aagtctataa cgtttctcac tgtgcccata agctccatgc
     7621 ccggtttcag gtctttaagc tccataatgt cgccgcttct cataagcggt ggcggaagct
     7681 cgtcacgagg gtcacgaccc ggcttttcaa gctcccctac tatgtccttt aacgtcggag
     7741 caccaatgcc gatatctgcg gcaagcttct tcatgcccat tgcattgact ttctcagcta
     7801 tctgctttac gtcgcccatg tccgcaaggc tcagcccaca tctgtcaata agagcctgtg
     7861 ctgccttgta tgactcaggg tgaacagccg tgttatccag cggatttttt ccgccacgca
     7921 ctctcaaaaa gcctgcgcac tgctcaaaag cctttgcgcc aagcttcgga actttcataa
     7981 gctgctttct ttcggtgaaa gctccgtttt cctcacggta ggaaacaata ttctttgcaa
     8041 ctgtcatatt tatgcctgat atgtatgaca acaatgagta tgaagccgta ttaaggtcaa
     8101 cgcccactga gtttacgcag tcctcaacaa cgcccttcaa agcctcgtcc attctcgcct
     8161 ttggcatatc atgctgatac tgtccaacac ctattgcctt tgggtctatc tttacaagct
     8221 ctgcaagagg gtcctgcaaa cggcgtgcta ttgatactgc tgaacgcagt gaaacgtcat
     8281 actcaggaaa gtcctcagcc gcaagcttgg aagcggagta aacggaagcg cctgcctcag
     8341 acaccaccat gtaagaaact ttctgcggaa tttctttgat aagctcggct gtgaactgct
     8401 ccgtttctct tgaagcagtg ccattgccta tggagatagt cgtaacgcca tatttcttta
     8461 taaagcccgt cagaatacgc ttagcacgct ctgtatcctt ctttggcgga gtaatgtaga
     8521 ttatcgcagt gtcaagcacc ttgcccgtag cgtccactac agcggtctta caaccgtgtg
     8581 catatcctgg gtcaagacca agggtaacag tgtctttaac aggcggctgc ataagaagct
     8641 gtctgagatt tgaggagaaa acctttattg ccccctcctg agcgtttgcc gaaagctccg
     8701 cccttatctc acgctctatg gacggataaa taagtctttt atagccgtcc tcacaagcag
     8761 ccgtaacaag ctgtccgcac tctgacttgt tttttacata tcttgacacg caggctctct
     8821 cacctgcgcc ctccgggaca ttcactgata ctttcagaac gccctctttt tcgcctctat
     8881 ccaaagccaa aactctgtga cctgctatct ttcccacagc ctcggcatag tcgtaatagt
     8941 tctcatacac tgactcggca ttctcgtcag acgcctttga gcttatcacg cctatctgcg
     9001 aaaacagcgt tcttatgtat tttctcaggt cagcgtcgtc agagatatcc tctgcgatta
     9061 tgtccattgc accctgcaaa gcctcatcag ccgacttcac gcccttttcc tcgttgataa
     9121 attttgtccc ctcgacctgc gggtcgcatt tcttatcctg ggtcaggata agttcagcca
     9181 gtggttcaag ccccttttct cttgccaccg aagcccttgt tttacgcttc ggcttgaaag
     9241 gtctgtagat atcctccatc tccacaaggg tctttgcatt ttcgatagca gacattatct
     9301 cgtctgtcat tttctcctgc tcagtgatag aagagattat ctcgccttta cgtttttcga
     9361 gatttctcag gtaagtgagc ctgtcagcga actctctaag cacctgatcg tccaacgagc
     9421 ctgtaagctc ttttctgtat cgtgcaataa atggtatagt tttatcgtca tcaaggagtg
     9481 ccacagtgtt gtctatctgc tcctgtctga gcttaaattc ttccgcaagg gtcttgttaa
     9541 tgtccatgtt tttaccaatc ctttttatta ctttttgcgt attatcacat ataatattat
     9601 agcacatttt cgataaaaac gcaacgaaaa tgcggtcgaa aactgtaaca aagatattat
     9661 ataatacata taggatcaat cagaaaatta ataaaagcag gcaaaaatcc acttgaaaaa
     9721 agggcataga tatgatatca tagaagaata aaacagtatt caggagtgtt tatatgctaa
     9781 atcagttttc aagaaccgag cttttattcg gcaaagaagc aatggaaaag ctcagcaaca
     9841 gcagggttgc ggttttcggc ataggcggtg taggcggata tgcagttgaa gcccttgcac
     9901 gaagcggtgt aggcgagctt gaccttatag atgatgacaa agtgtgtctg accaacatca
     9961 atcgtcagat aattgccaca agaaaaacag taggaaaata caaggtagac gtggcggagg
    10021 agcgtgttca cgacatctgc cctgacataa aggtaaacac atacaagtgt ttttatatgc
    10081 ctgacacagc ggatcagttt gattttactc agtatgatta cattatcgac gccattgaca
    10141 ctgttacagg caagctggag ataataaagc gagcagacgt cgcaggagta cctgttatat
    10201 cctcaatggg tgcaggcaac aagcttgacc ctacagcatt ccgtgtggcg gacatttatc
    10261 agacaaaggt ctgccctctt gcaaaagtca tgcgacgaga acttaaaaag gcaggcataa
    10321 agtcgcttaa agttgtttac tcagaggaac aacctatcag accgatagaa gatatggcta
    10381 tttcatgccg tcaggactgc atttgtcctc ctggcacagc aagaaaatgc acagtgcgcc
    10441 gtgatattcc aggttcaaca gcatttgttc cgtcagtagt gggacttatc gtggcaggag
    10501 aagttattaa agatctggct aagaaataat acaaacaaag gacgaaccat tgatcagctg
    10561 tggtataatg gttttaaaaa tcagtcggga aaactgcccg acaaatcagc agttgataag
    10621 gagtacagga atgaaaaata gaataatata ttgccttaat ttcctttgga caagcattat
    10681 tgcattttcg tttccaatat gctttggctg gatttttctt gatataacag gtcattcaaa
    10741 aggatatagt tataatttag gctctgaaaa agatgtttca atattattgg gctgcattga
    10801 gttgttgata tggttggcat tatccttacc atcatatatt tatatttttc ggaaaacaat
    10861 cactaaaggg aaacgaaact tgtatgtaat aatcgcttta tatatagctc ttgctttaat
    10921 ttgtatcatt gtttcaggcg gaatatctgc ctatttaaaa gcagttttca atatatatta
    10981 atcagattta tctcaactat tacttcccat ttatcgagca gatcacttat cgcagcacta
    11041 ttatttgaat ttttcagaaa ggaaaaaatg tnnnnnnnnn ntgccgcatt atatcaaagc
    11101 aaaaaaagcc aaaaacacat atctggattt ccattttttg gtgggtttat tcttgctgta
    11161 gcatttctct tctctcctat taaatggtta gcttttctag gcttcattga ttatggtttg
    11221 tggctcctgc catatgtatt gattatggat tattataaca acaaaaaatt caaaaagata
    11281 tatatgcagc agaattttga acaacgcatc agtgatgaat caaaagaatt acgtatcaga
    11341 atttctgaaa gaaatgaaga atgggtacag ccgtatatta cgaatttagt ttatgaactc
    11401 aaagtgccta agctgttata tgctgtttgt acagaccaaa atggaaagaa atttttactt
    11461 attgataaat gcaaaagaaa aggcaacatt gaaatagttc cttttgataa taatactatt
    11521 ctgcttacag acctcaactc aaaaaatgtt gattattcag tagaaattga aatcaaagac
    11581 aatccataaa tagtttccca tttatcgaac agctccctca tcacagcact cataacgtgt
    11641 gctgtgtttt ttagacaaac agtgcttccg cacacttaca ttaacgtaag catacgaaag
    11701 cactgtttcc ttacattaca tttacaaact tcaatactaa atcatatata caaaaaaaga
    11761 gctatagcaa aagctacagc tctttggctc cccctgttgg actcgaacca acgacccttc
    11821 ggttaacagc cgaatgctct accgactgag ctaaggagga atatgactaa acagagaaga
    11881 atattatctt ctctgtttag taaggtgtcg gcagcaccct atcttaccgg gacgttacca
    11941 tccaagtatt ttcggcg
//
LOCUS       DAWEMR010000073        11804 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2304_length_11804_cov_12.900758,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000073 DAWEMR010000000
VERSION     DAWEMR010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11804)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11804
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2304_length_11804_cov_12.900758"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5522..5531
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3551 a         2278 c         2409 g         3556 t
ORIGIN      
        1 atcgtagatt taattattac atatggaata atagtgagta tattattatg ataaactcaa
       61 atgttagtaa aaatagacta agaattacaa ttgaatttgt gcaaaaggta gaatgataag
      121 tagtaatcta aaaaaatgtc agaaatacgc ctccagtgtt gttatgtatt attgaaacgg
      181 gtaatacaga gtttaatagt agattttcaa aaggagtttt ttcaaaaagt gtgtttgccg
      241 ctgatatttc tacatatgac aaatacacaa gcaatgtcag cacagctttg gcagtttagg
      301 ttacaatgct aaaatgcaga agtgttgtta caggatagtt aggtattacg aacaaaataa
      361 ctataacaca ggaatttcaa aagaaaagcg gttttacctg gagttcaata aacggcagag
      421 gcactacaaa gtttactttt acgcagtttt tgaacaatct tattccaata taagccgtgg
      481 tagaaacaaa gcaaagacca ttgctaaggt ctatagtgga acagactata aaacgtattc
      541 cacttattat tgatacgatg aatgtaggtt ccggagatgg tagaaatcgc tcttagaaat
      601 tacaacatta gtaaaatggt acgacgacag tgtaaaaact ataaggtgat actgcacaac
      661 accgtcagac tttatttgtg tggcgttgca ttatgttata atcagacaat attaaacttt
      721 ttatcttgtt acgctgtgca atttggtata aacttaataa tctaagggag gtaaaatatg
      781 tcgttaaaat atcatcagct tgatagttct gttgagaaac agataaagga aaatatatca
      841 aagggaatat tgagttcata cagatttatg gacgaaaatg ctgtgcgaag aaatatggaa
      901 aaggacaaaa atagcgtact tcgtccgtct tttggacgtg atatagagaa aattcttcat
      961 ttgcctttgt acaacagata taatgataaa acgcaggtat tttcattcta caacaatgat
     1021 gatatcacta gacgaggcct gcacgttcag cttgtgtcac ggataagcag aaatattgga
     1081 agaatgcttg gccttaatct tgatctgata gaatctatcg cacttggaca cgatgttgga
     1141 cacacacctt ttggccacgc aggagaacgt tatctcagcg agcttttgca gaaagaaacc
     1201 ggacggcatt ttaatcataa tgttcacagt atacgtgttc ttgatatttt gtacaggcga
     1261 aatataagtt tacagacact agacgggata ctgtgccaca atggtgaatt tgagcttaat
     1321 gaatatcgcc cccaaaaggg tctgacattt gagcaatatg acaataatgt tgagctttgt
     1381 tatactgagg gcggagaggc aataaaaaaa cttgttccgt caactttgga gggctgtgta
     1441 gtaaggattt gcgatatttt agcctatatc ggtaaagata ggcaagatgc gataacggca
     1501 aaaatattgt cggacgattc aatattttca tctgaattta ttggcagcaa aaatgctgag
     1561 atgataaata atcttactgt tattttgaaa agaggaatga tgtttgaaac aaaaattcta
     1621 aaaaacagca tcatctattt catagtcgca acacatactt gtgcaataaa atagatgcac
     1681 acctcgaaaa gctcgtatta tcgcggcaca aatagggaac gaccctaaac ttatgtatca
     1741 cggagagtca tctgatttat tcagactaat gctctccgtt ttctttttgt ctactttaca
     1801 attccgatat gagtaaagta aatatcgatt tcctgcgtga tactgccgtt atcgtctttc
     1861 agcttttcgt gaacctcgat gcgcgataca aacgtgtgta agatttcctg agttaattct
     1921 gtaatatctg tgtacttttt agcaagcgca gcaaatcttg cggtgttagc cgtctgggat
     1981 tgaagctcct gcatcgtatg cttcaagtct gatatttccg ctttaagctg attctgctca
     2041 tcagtgtatc cctgcgacag cattctgaac tgatcatcat caatcgtacc aagaacgtga
     2101 tcttcgtaca gctttctgaa aattattccg acctcggcaa gccttttctc gctctttttc
     2161 agttttttgt tagcttcgga cagctctttt ttagcagcag tatttgactc ccgactgata
     2221 tactcagcaa aatcatctgt acgttctctc gcaaacgcag tcacattccg tatttcatgc
     2281 agtacgattt ccttgagatg actttcctta ataccgtgca tggtacattg ttcccgacta
     2341 ttttttcgat acgaagaaca gataaatttc cagttttcag acttctctct tgcgtgtctg
     2401 tgaaaatata acttctgttt gcagtccgca cagaagacta ttccggcaaa aatacttttc
     2461 tctccgctcc ttgtgaacct acgcttgctc tgcctgagtc gctgaactat ctcccatata
     2521 ttaatgtcga ttatcggctc atgagtattt tcaatcagct tgtgctcgct tttgggcagc
     2581 tttaatttcc gatggtcttt gtatgacgct ctgccgaacc tgaaattaca agtgtgtccg
     2641 agatagctga cgtcctccaa aatatttgca actgcggtcg gattccagcg gtagggatag
     2701 cttgtatcaa agttagaaat aacatttccc gtttgactgt actcgtacat cgcaggagtg
     2761 tatatctgtt cttcctcaag cctgtgcgcg atctgagccg gacccaagcc atcagcgcat
     2821 agttgaaaaa tatattttac gaccagcgct gtttcgggat tgggcagaat tttgttgtga
     2881 tcctcctgag atttcatgta accgtaaggc ggacgtgaag cgactctctg ccctctttcg
     2941 gattttatta cacagcttgc cttaattttt ctgcttgtat ccttgacgtg gaattcgtta
     3001 aaaatgttgt agaatgccgc catatcgaca gcggaggaat ccggattatc ggaatcaata
     3061 ttgttattga tcgcaatata gcggacattt ctgcgcggaa aatattcctc tgtgtataat
     3121 cctacgtgca gatgatttcg accaagcctt gagagatcct ttgtaattat tgtgccgatc
     3181 ctgccgttgt ccacatcgtc tatcatgcgt ttgaatgcag gacgttcgaa atctactccc
     3241 gtgtacccgt cgtcaatgta gaacgcggtg ttgtaaaatc catgttcatc ggcatatttt
     3301 tgaagtatat ctttctgatt tgaaatgctg ttgctttctc ccgcaagctc atcctcgtga
     3361 ctgagcctgc aatacaaagc cgttatctta ttcgtgtctg acattattaa cctctctttc
     3421 cgtccattat gcggactctg tcaaaccgat tgagattaca gatagtatat cacaatcgat
     3481 tctgaaagtc cagcgataat ctataaattt atgataattt ttcagcggcg gatctcagca
     3541 aatatttcag cttgtcctca gctgtggatt ttgaggtcat atcaaacacc gagttgatgt
     3601 tgtaaacaat tccgtcaatg atctcggaac gacttttgat atacttatcc tcagtttgcg
     3661 ttatgctgcc ggagcaacag ctttcgtttc tttctataat atttgtgtcc atatacctcc
     3721 tgctctccga tatatatcgg aacaaatgtt gagaaaattc gtgtaaacaa atcagttcag
     3781 tcctttcgtt ttaaattttt tgtgcgaatt tgatacgcac cgattttaca attcgttatc
     3841 atcaacctca taggcgttaa cgataacatc accttcgcca tattcgcaag aattttcagc
     3901 tatattttgt tcggcaaatt cacggttgtt ccttgcaatc aaatcgtcaa ggttaacttt
     3961 accccttgtc ctgcgaacgt tacgcaccgc ctccttgcga tactccgcta taactttcgg
     4021 aggaatatca gccagctccg acagtatcat aatcacaatt tctagctcaa ctgcgtatct
     4081 gaacaagcct tttgagattt tagttatccc tgcctcgcta cctttcacga tttcatctgc
     4141 aagatttttt gcgatgatat tcttctgcct gccgacttca attgaggaaa catactccct
     4201 tatcgccttg gctataaatt ctgaacgtga gtgcgaagtt ttctccgcat actcatcaca
     4261 aagttccaaa gtttcattgt ctaagcttat tcctattttc tttgccataa ttacctccgt
     4321 ttcctgcggt attacctttt ggtgcacact ccaaagggcg atatttccgc atttttattg
     4381 attttttact tgctgcgaca cctccgtttc atacttttgc aaattaagac tccttttact
     4441 cgagagccga aaacctccgt aatttcggca aaatattgcg gtcagctttg ctgtccgctg
     4501 tgagtgcgac accgtcgctt taacttgcaa attattagtt atgcttactt taacctctgc
     4561 atatccggta tttgtgcaga ttgacaatcg ccttttatta tcagaattaa aacctctccc
     4621 acatatcgcc cacagcggct cacaaatagc ctgtgtcggc ttttaagctg tttctgctag
     4681 acttacccga caaaaattca agccacgcaa atcggcttac gttgcctttc tttgcgtttc
     4741 aaacggcgga atttcaatcc ccttattcgc aagctgactt cttattttat tagcagacat
     4801 atgcagtact tcatcaaggc tcattaactc gatttcataa acttcattag cggcagggtc
     4861 agtaacgtca tcaacagggt cggcagaatc acaaacagtg acagcggatt tttctgcctc
     4921 aacttcgtac tcatcaatcg caagcaacgt atttaaaggc ttttcaagca tttcactgtt
     4981 accgcaagtg acagcagggt cagcgttttc acactccttc ggcataagat ttctgccgtc
     5041 actgctgtca cttttcatat cgtaattcag aataattatt cgctgtccgt tgctgcgttt
     5101 ggattcaaac gttactccgt aatccagcaa ttcataagcg tgttgaatta aatctttttt
     5161 gacacgattt gaaaacattt ctttgtccag cagagcagat aacttttcag caagttcagt
     5221 agcagagcct ttaaactttt tctgagttac cataaagtcg tgaacagcaa aggggaaata
     5281 gtcgggttcg atatttttaa tctcatcaga cactttccat ctgctgcttt caaacacaac
     5341 attgatctct tgattttcaa tatcacgacc tacgcaatac agtttagctt tccgacttcc
     5401 acgcttgctt tcaatcagaa ccatacttcc gtcaacgcaa ccgctcaaac ctgtactgcc
     5461 tgaaatcata ttgaacggat cgccgtcgga acactttcgt gtgtgatgaa ccagaactat
     5521 annnnnnnnn ncaagagctt acagaactga caactctttg taatctgaac cgtagctcga
     5581 ctccgattcg ttacgcacca tttgtaaagt atcaatgaaa acaattttca aatcgtcaaa
     5641 ccgtttcctg cagtattcga tttgttcagg cagaccgttt gaaatagact ccgccataat
     5701 tgcaaaatga agattatcgg acggatcgtc tgtcagttcg tacaacctgt tttgcagacg
     5761 tatcaggcta tcctccaggc taagatagac cgcatgaccg caggacgttg cttttccaag
     5821 aaccttttct ccttttgcaa tggacaggca catatccagc gcaagccacg acttgccgac
     5881 cttcggcgca cccgcgagaa taaataagcc ttgcgagatc aatccgtcca cgcagtactc
     5941 aatcggtttc atcggagtgt tcataatata ctcgctgctg taaattttta attctttcat
     6001 attatcgacc tccttccggt atgcaaagcc gctgacctcg gttctgcaaa caaaaaacgc
     6061 cgcagattta gttttctgcg gcgcgttttt aactctctga tattattata tgcggaaatc
     6121 cggaaaatga ataaaatcac gtaacattta attttcgctc aacacgacat tttctgttgc
     6181 atattacgac aaaatgtgat atacttaatt tggaaactgt aaaaacgaca gccttgaggt
     6241 gtaattatgt ttaacgatat ttgtttttat gtcaaaggaa atatgatcga ggtcaacggt
     6301 cgggactttt tactgggcga gctttcggca tcatgtatga atatacctcc tgccgaattc
     6361 gaagagatat atgacaagta taaatcggcg gaatacataa tgaaccatga aatcaatgct
     6421 gaaaaggata ttgccgttga atatataccg ccctcgaaag aagattggct caagctgaac
     6481 ggaattatga ttgaaataga tacggctttg aaaaatcata aaatatttca ggtttttgat
     6541 actcagaatg ctgccggatt tttcgaacga tttactgata tagacgatac aatgattgct
     6601 cacgaaaccc gggagcttta ttacaagact gcaagtttgt acaaacctgt gatcgacgat
     6661 atatttaatt tcaataaaac tatgtattac tttgtaaacg actttctgtc acatctaaaa
     6721 aagcttgatc ctgaaaattt tgccgccgct tactatgact ttcttacaaa tcctatggcg
     6781 tacaagatga ccgcaaatcc gatcatgaac gaatatatgt cgtatacaag cgctgatttt
     6841 cttgaaatga atatgatccc caaagagata accgagggct gcggagagta tgtaattgcg
     6901 gaatactacc atgttgatag attgcagagc tttttaaagg tcgattttct aaaaggtctt
     6961 atggcaggac atcatatacg cagatgcgag cactgcggac gattttttct tatgaccaag
     7021 ggctacaaga caagatactg cgacaagcct gcgcctgaaa atccgagatt tacctgtaat
     7081 cagatggctt accggactct gcgcgtaaag gaagaaaacg cagataatcc gaaatatcag
     7141 tcatatcggc gctgtcttaa cagagttatg cgcagctatc agagaaaggc agtcaatgga
     7201 gaacaaaaat ctgctctctt acgcaaggcg gaagagcttt accacacagc aatgacctct
     7261 tccgaattca gcaatgagga atttgaacgg caaatgcagt ctgaaaatct ttacaaatta
     7321 tgcggttttg acgttccaaa aagaggcaga ccaaaggcgg ttaagaatga caaatgagga
     7381 attgattcaa aaatattatg acggcgatat gcaagcgtta tatgatttgt acgagcggaa
     7441 tgtcggattc atacagagcg ttgccgctgc ggctgcaaag gactacaaaa actatctgcg
     7501 tttcagcgat acctttgacg atcttgttca ggtgggctgc cttacgttct ttgagaggct
     7561 tagggcaaaa cagtatgacg caagtaaggg cggcctgctg acatacctca ctccccgact
     7621 tcgctatgca atgcaggagt ttatcgaaca atttagttct cccataggac tttcacattc
     7681 cgatttttgg aacattcaaa gatgccgtaa gctgcataat gagggtatgt ccaattcgga
     7741 tattgccaaa gaacttggta tggacaggaa aaccgtgcaa agctgtcttt cattttcctt
     7801 taagacggaa acgctgatga tccgaactga aaccgagaac ggaatagaat actttgaaaa
     7861 tccgaagctt ggcgttaacg atctgcaccc cgataacaag gtttacattg aagtctgtat
     7921 tgagcttttc aggaaattgt ttgataactt atcggccagg gacagagaaa ttctcggcag
     7981 aaaatatggt gcattcgggt atgaagagac ctccgttaac gacattgcgg actatatgct
     8041 gatcaccaga aacgctgtaa acaaggctgt taattctgca acagagattc ttcgcaagga
     8101 ataccataac gggagtaagc tgaaatggtg gcggttgtgt aaccgggcgg ttaaggatgc
     8161 cttggagggt aggacagcat aggaatccgt tgaaatatct gacgagttta aggcgaaatt
     8221 acgggcgttg gcggagtttt tggttttgct gtatgaaaat gataaacaaa aataaatggg
     8281 gggcattatg cttgcaatat acttaacctt aattgataac gaagacgata agaaatcatt
     8341 tgaggattta tataatcaaa ataggtctaa ggcttatgcg attgcgttta atattttgaa
     8401 aaataaaacg ttagccgaag aagcttgttc ggagactttt tttagtctcg caaaaagttt
     8461 tcaaaaaatt aaaaatttag aatctcacaa attggactat tatattgtca ttactgttag
     8521 aaacgtgtct ttgaatttac tgaaaaaaga aaaggaacac attaaagcta tgaatttaag
     8581 cgaagatatt cccgaattaa cagacgagac gctttgtgac agaaattacg ataatatagt
     8641 tgattgtatc aagcggcttt catataccga tcaggaaatt ctttacctga gaatcactct
     8701 tggcatgagg tacagtgaga tttcgttagc actgcatatt tccaatgcgg cgtcacgaca
     8761 gcgttttcag cacgcaaagg atagtcttgc aaagctgctt gaaaaggagg gcatttataa
     8821 tggataacta ttcaatatta tcaagagcta taggcgaggc ttttgaacct aagcttgatg
     8881 aatttattga ctctgtaaat tttgatgtta atgccgagtt ctcagaaaag ttcgaacgta
     8941 agatggacaa acttatacga agaaggaata aaccgtattt taatctgata tgtaccggag
     9001 gaaggcgagc ggcgtgtata gtggctgcca taataatttt gtcggcatct tcactgagcg
     9061 ttgaagcggt acgtgaggcg gttaatgatt tctttatgag tattttcgga gatcacactg
     9121 ccgtttcggt aaacaacgct actgaaaaag attatccggc aactatcaaa gaagaatatg
     9181 cgatctcaaa tctgcccgac ggttttgaat tatctgatta taaccgagat agcggaacaa
     9241 tattcgccgc ttattttaat ggtgataaat atatcttttt cgagcaattc gttcatgata
     9301 gttactcagg ttattttgat aacgaacatt ctgaacttga atactacact gatgaatcag
     9361 gacaggaata tcttattcaa agtaccaacc atgatttctg tattatgtgg gataatggta
     9421 ggtatatctt acaaataaaa agcaatatga acaaagacga tgtgctggaa ttgtgcaagt
     9481 ctacaaaatt gaaataaatt ttgaaaaaca gctcataaag ccgaatattt tgttgtcatt
     9541 aagagtgtaa gaggaaaaat ttctcttgca ctcatttttt gtaggaggag atattatgaa
     9601 aaaaattgta cccattttta tggcttgcct tgttttctgc tcagttggag gagctacagt
     9661 gtcggctgct gttccgcctg ataatggtag tgttgtttct ccctgttatt tgtatacgga
     9721 tagtgtcaat tgtaatttga ctatttccgg cggtgtagca tcttgtaaaa gcacagttta
     9781 cggaatatcg ggaacaacaa ctaaaatcgt tgtaactcag accttgcagg taaaagacgg
     9841 aatgtcatgg cgcaaaataa ccgactggag caaaactttt aacagctggt attgcagtta
     9901 tcctaactca ttctcgccga ttggcagcgg aacctaccgt gttaaaacag ttgcaaaggt
     9961 ctacaagggc acatcttacg aaacggtcac tgtttacagt gcggaggttt tgtattaatt
    10021 tacagtttat gctttaggca gagaggtttg gtgattccaa tgaactaaga tatgtgaatt
    10081 attggttttt ctgacgtaga accaatactg aacaatcaca atacggactg ataatatgca
    10141 gtatttcgaa tatagagttg aatatatatt tgcgtattgc ggaaatggag gagaagttat
    10201 gaaacttaaa agcaacttca aacgaatact ttccggcgta atgagtgtag ctatggcggc
    10261 aactctttta ccgagtttgc cggcagtagc agaggaggca gcagaaaagt atccctacac
    10321 aatgtttgcc gcttctgata cagaggggtc tatcaccatt aatgctaaca atttctgtgt
    10381 gaatggaaat atagcgacaa acggcacaat agtttcaaat gggaatttga acgttaatgg
    10441 tacgaaaact gagaatgcag gcaaagaaat gatatatatc tttgataaga tcgattcagc
    10501 ctatttttca ggcaataata ttgatgaata ttcacaggat tacactcttg aagaaataaa
    10561 cataaacatc aatgatccgc tggaggttga gggtaatgct actcttacag gcaacataaa
    10621 tatcaacacg gcgcttaaag cctttgaaga tgttaccctt aacggcgagg taaaaaacac
    10681 aaacaattct gtcatctttt caaagtatgg agatattgtg attgacagcc agaacgttaa
    10741 tcttaatggt ctggtatatg ctcctttcgg caacgttgag ataatggctc agaatctgaa
    10801 tctcaataat gttgtcatta ttgcggatac aataactttc aactgtccaa gcgtaaacgc
    10861 taactatagt ggcaatgctg gcgaattggt tggcactgtt tcagagcctt tggatatacc
    10921 tgtcgatgaa tggcagtata tgaaggatga aaacgacaat ggcttacctg atttctttga
    10981 agatatgaat aattgggagc ttctcaaaga taccgatgga gataagcttc ctgactgcgt
    11041 tgagcagtat cttggaagtg atagcacgtt ggtagatacc gatggtgatc ttcttgatga
    11101 ctattatgag gtgtttgtta ccggaactga cccgactttg attgatacag atgaaaacgg
    11161 aattacagac ggtgatgagg attttgatga agacggatta acaaatcttc aggagtatga
    11221 acttggtaca gagccttata atgatgatac ggacggcgac ggacttaatg acggagaaga
    11281 aatcaatact tataatactg atccgctaaa gaaagacacc gatgacgatg gtttagaaga
    11341 cggcgatgaa atctatcttg aaactgatcc tacaaatccc gatacaaacg aaaatggtat
    11401 tcttgacggt gatgaaaagc gtttacagac gtttattcac aaggttgaaa acgaggattg
    11461 tgccgttaca gaggttcgtg tatctatgga gggcacgggt aatctccaga aaaccacaac
    11521 cgttgagagc attatgaaca aggatatact ctgctcagag gttgtaggtc ttgtgggcga
    11581 gccttttgaa atcaaaacga cttcgcagtt tgataaggct acgcttactt atgtgatcga
    11641 caagtctaaa cttggcgata ccgaatttga caatctgcta tttttatggt atgatgaaga
    11701 aaatgacaat tttgttgagt tggacactat gcttgatgag gagaactcta ctgtaagtgt
    11761 ggagactaca cacttcagta agtatatgat tgttgacgga aaag
//
LOCUS       DAWEMR010000074        11595 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2347_length_11595_cov_12.307886,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000074 DAWEMR010000000
VERSION     DAWEMR010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11595)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2347_length_11595_cov_12.307886"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3159 a         2360 c         2455 g         3621 t
ORIGIN      
        1 ttagagttac accattttat tatttcatgt gtctactcta aggggattat atcactgata
       61 tgtgctgtca tagtttatgc tgtttgaaaa cataagcgga aatatcgcaa atatttctat
      121 aaggctgact attacaaaca gtattatcag aagcagatta ttttttatct gtgttttgag
      181 cgttgttagc gacatagtat aatctccatg tgtttttata tgtgtaaggt tcggtgaacg
      241 ttccaagata agttacatca taatcgtctt ttgagttgaa atggtcatca agctcataag
      301 gcgagtcagg gaacagatct ccaaaaataa acagtttttt gctgtatttg tttatgttat
      361 caatgcttcc gatatcagtt acttcagtag ggaaaacgtc aagagtgttg agtacgcacc
      421 atgtatcatc gcatacataa tgctctgcat caggaaaata gtacatcatt attccaagcg
      481 accattcatg taaatgaaga aaagcaatat catcatctcc gttttctttg atagtttcta
      541 tcatatcaga aaaatcagag ctatctaatt gacttttaac attgccgaaa ttaatgcaga
      601 agcttacaat aaacagcacg atacaagcta agaaaagctt ggtatcaaaa agtttgccta
      661 tcactactga cagcaatatt accataagag agcttaaaat ataaaaatat ctggtggcga
      721 tgatagggta aacaaggcga gtgaacaaaa ttagagtgat aataggagca acatacatga
      781 gtccgcagta gagaacactt acaaactcgg cacggtgagg tgcaagcttt ttacggatat
      841 catttaaggt tttgcagtta cgcatcttta ccacatggat aagcaacatt atggcgataa
      901 aaatgaatat caggtaagtg acaagtccgc ttgcataacc aaagtgaata tcatatgtcc
      961 aatctataac aaggctactt gaaattatgg aatttgccca atagtttttc ttaacatttg
     1021 aaaattgctt cattacaact atcagccaag ggcaatagct taatgcgcag acaactccgc
     1081 taaggaagaa ataaagagtt ttcttctttt gctttgaaat aatagtaaaa gcaaaagata
     1141 ctatgtagaa agacaatgcc gcaagcattg atacattgtg agtatacatt ccaaggatag
     1201 caaaaactgt catgcagata taggcataat gatagccctt aaacagtgcc aaatacatat
     1261 aaatagcaca agctgctgta agaaaataag caagtacggt tggacgtatc tcaggaacga
     1321 gtaaaaagtt gacataagaa agcgtgtaaa gcagcataca tatgactgat gcacgtttgc
     1381 caaaagctgc acggacaggg aatgctgcaa ggaatatcaa tccccatatt attaatatgg
     1441 aaaatgacct cattgctaga agtgaactgc cgaatatgta ggtccaaagt ttaagaagca
     1501 aggtataaag gggaggactg tagtcctccg gcagaagttt catgatatcg cttaaactgt
     1561 gtttggttaa aaaatactga taggaactgt catagtttat tatgtctgca ttgaaaggcg
     1621 gtagtatatg tataactgat actgcaagca ttacaagagc caatattaaa atgccatagc
     1681 gattaaaaaa agctattgat ttgctgataa catctgagtt gtcacttttg tctgtttttg
     1741 ttaaagctgg catttatcct gactcctatc aacataaata atgtttttac ataccctaga
     1801 ataaatttga aaagctgacg ctttgattcg ccataaagac gtttttcaaa gactattggc
     1861 gtttccttta caaccaattt gcggttgttt attttcattt taagcagcac ttcctgcaaa
     1921 atatcataat tttgacaggt gagcttaacg gctttaagct gctttgtgtc atacatacga
     1981 aagtctgtgg aaatatcctt tgcctttatt ccggttatta ttctgaaaac aaagttaagg
     2041 atatgagaca ttattataga gacctttgaa tcgtttgtct taccgccttt gacatagcgt
     2101 gagcctataa caacatcagc gcctttgaca aatttcttat aaatgtcagg tatgtatatg
     2161 gggttgtgag agccgtcgct gtccattatg agaaatttat cataacgtgc cgccttgata
     2221 cctgttctga aagctcctcc aaaatgcgga agtctttggt ttacatatct cgctccgaat
     2281 ttcttgcaca cagcaggtgt gtcatcaagg ggcttcatag tatcaacaat tatgatctca
     2341 tattcttcgc cgatcttatc aagctgttgc tttatcttcg gaagaagtac ctttaaattt
     2401 tctgcttctt tgtaagcaag aagtactgcg cttatagcca ttatttatca ctcaattctt
     2461 ataagttatc gttctgttta gaaagtattg cagcaggcat acaaatatca ttaccacaaa
     2521 tttcaaaata taaatattca tactcgattt aagcagatgt atcagcaggg tagatataag
     2581 cgagcctgcg atacatatgg ttgcgtatga tgcaaaagtt ttcatgtact cgctgcgttt
     2641 tttgaaattt acaagtgtat ttgtcataaa cgtaaaaata aaaccacaga cattaccaat
     2701 aaggctgcat atctcaggct tatcaaaaac ggcaaaatgt atgaaaattg cactgataac
     2761 ataatctatg accgcagcgg atgcaccaaa aagagcatag agaataaagt tgcggtattt
     2821 tttatataag ctaacaatat gtgtcaatca aactttctcc caccataaaa atatatactt
     2881 tgtttataat tatatcacat tcattcgcaa aaagcaaggc tttttgtcat atttgcctct
     2941 attttacggt aaatatttgg ttaatggtta atgaaagaat attgtacttt ttggaaatct
     3001 atgatattca acatcgtttt ttacaacaaa tccgataata ttgacgataa aaaaaactcc
     3061 ccatgcagaa aacctctgca tgggggatat tttcataaac gataaaaatt acttcttagt
     3121 agtaacactc tttgaagccg accaagcgcc ataatactta gtacccttta cagttgtgta
     3181 agaacgaaca cgaacatagt atttcttttt gcctgaaagc tttgaaatag tcttggtgtc
     3241 ggtcttttta tttgttattg tgattttctt tgcacttgtg aactttgagt tggtagcgta
     3301 ctgtatctcg tatccagtag cagagccttt ctgcgcccaa tccacaaaga atgccttgct
     3361 cttagccgtg agcttctgta tctcctgctt ggcaggattt atcttgagcg tctttgtgat
     3421 agtgcctgtg taagagcctt ttcctgcgat ttttactgtg gcagtgccta tgctcttgtt
     3481 gcttgagtag gaaactgtat agtcagtgcc atttttcagc gttttgccat tgtatttagc
     3541 agtaatgctc tgagtaatat ttttgcccgt gtatgacttg tttgaaatgc ccgaaactgt
     3601 ggctttcttg aagttatttt tgatactgta ggtcttagaa actgagcctg tgtagttgct
     3661 cttgcccgtg atttttacag tagctgtgcc taccttggtg ttgtttgaat aggaaactgt
     3721 gtaatctgta ccattcttca aagttttgcc gtttagcttg accgttacac caggcttctt
     3781 tgccttgcca tcatatgcgt aggtcgaagt ggaaagtgtc acgcttgcct ttgaaatgct
     3841 aattcttgaa agtgcaggaa tctcagccgt ttcaagaact ttcttgcaga ctgtacattc
     3901 cttgtgtttt gagcctttaa ctccgataga agccgttttg tcaactatcc aaccgcttga
     3961 cttgtgacct gtggctggga tagtttcggt gtagaagtca ccgcatgagc aggtgtattt
     4021 tactgtgcct gcggtggagc aggtggcatt gctgagaact gtttttgtat agctgtgttc
     4081 atgataatcg tcaagcgcaa atggtgagag cttgtaaagg gtcggtgctg aattatttgt
     4141 gacataccat tttaccatgc cgtcactgca tacaacaggt tgacaatctg aaagtccgaa
     4201 ggagtgagaa atggctttgg taacagtttt gccgtttgag tcaactgtca ttgtctttgt
     4261 ggcggtcttg cctgtggaac tgttgtattc ttcccacata acaaggaaca gattgtcatt
     4321 tatctttata agctgagggg tgcggcaggt cactttactt gatgtgccgt agtttgtgag
     4381 ggcaatgttc tgtgtgccgt tgaaaagctc gtctgtgacg cttatgtaaa catttcttgt
     4441 cttacaactg cttgaaacgt cttttgcata ggctatcaga cagtttccgc tgccaagctc
     4501 aaaaccgcct attgaagcgc ccgtgtagtt gcttttgttc atatcgaact taacagggat
     4561 agaaacgctc acggctgttg attttccgtc agccttatat tttgtgagag taatgccgtt
     4621 tacagaaagg taggagccgt tcattgtgta gttgcttgat tctgagtggt caactctgta
     4681 gatgtatttt ccgctttcgt cagctttgat gaattggttg aatgagtggc taacatagcc
     4741 gtctgtaaga tttgaaacgt catacattga gtcggtcagg gacatggagg attcgtcaat
     4801 ggtgaaaagc atatttgcct gatgatttat gccgtcactg tcagcataca tctcatggca
     4861 ggtgtaaaca taaagctttc cacccgcttc ttccattctc agtgagcctg ccgagaacgg
     4921 ctttgttgta ttgacacctg agatactgca tgaatttacc ttgctccagc tcttggtgta
     4981 tttgactact cttacaactt ccttgctgtc gctttcgaaa gtgttgttct gaccgaacac
     5041 gagatagtta tagtcttttc ctgaataaaa gccaccaaat aggttaagct cttttgaaat
     5101 agtgcgttgt gatataagct tattgccgtc tgcggaatag ttttctacca caatgccgct
     5161 tgatgtgttt tcaagccttg tgagcgttcc gtcgctgttt tgtacaagat aagagtttgt
     5221 gaccgatgac catgtgttgt aatggttatc tgaagcattg tctgttgtac gtccgctgag
     5281 tatagtggca gagccttttg tgtcgctatc cataggcgta actgtcactg tacagcttgc
     5341 tttcacactg ttgtcggtgg tggcggttat tatgcatacg cctgccttgt ttccgtacac
     5401 cttgccgtct tttgtaaccg aagccacaga attatcactg cttgaccatg tgaagccgta
     5461 gtctatatcg gaattactct ttgcagaaag tttaaggctg tcgcccgttt tgagcgtggc
     5521 agaagaattt gaaatcgtaa cactgctagg cattttccaa gcaatattct ctgagtagaa
     5581 attgccgtca gtatcacgct ctatggctgt cacagtgccg ttcgtataat acaaagcgaa
     5641 aacaggataa gccgttttat atgaagccac ctgcttgaaa tcattgttga actccataag
     5701 agtgttgttg tttacataca tgattgtgct gtcattttcg ctgttgtata cagttcttgt
     5761 tccaacgctg ttgtagagat catctttgtt ttcatgttcc gctcttgaaa cgcttccgaa
     5821 gagagataat gtattatctg ttgatgtatg gaatttggca gattccacga tgtttacagt
     5881 gccgcttgca gaagaagtcc acgcaagctt gccgttgcca agcatttgtg cgttgtcata
     5941 atgataatcg taatatttct gatagaaata gtcgatagat ttatcagaca cggttatttt
     6001 gttatttgaa atgtttccaa agccaagggc gttcattttg tgagtatagc cccaataaac
     6061 gtaatcagcg cttctttcaa aatagaagtt tccgtttgtg ctgtcaaagc ctgagaactt
     6121 tgttatggca acttcggtgc tgcaggagga cagcttattt ccgtttgagt caaggacaaa
     6181 tagattatcg cctttttcag caccactgct gtctgttaca tagtaatata tcctgccgtt
     6241 atcgtcaacg ccaaaacagt ttgggtaatt taaaatacca ttgggggtat acttgctcac
     6301 attaacgctt ttggtcacct tttccgtatc ggtgtcaaag gtctttatgt aataagaata
     6361 tgacgagccc ttcacgctgg aattgtatgt gctggtatct ctttcgatat aatagatctt
     6421 ggagttattt gcatatacat ttataatgtc accaagcgtt gctacagttc tcacggagtt
     6481 gttggaaaag ctatagaatt taattagctt cttgtagttt tccacagtat aatagtatag
     6541 tccgttttta gtggcagaaa tcacgtttac gaaaacctga tcggagctgt taaaagtcag
     6601 aaaatctgac ggcattttta cggcgccgtt tgcagaataa gcctgagctg atatagcggt
     6661 gttccccaat atgccgttaa tgctttctgg aagcggaagc agtgcggcag aagctgttat
     6721 aacaaaagct gtaagtcctg cggcgatctt tttagtcaac atatttatcc ctctttacta
     6781 tgatctttac cccgacccta tgtgtatatt atactacttt ttttgcctga tttcaagtaa
     6841 aaagctccgc cacagcgaat gccacggcgg agtttttagg acttttggaa gaaattactt
     6901 gttgcctgct gtcttgtaag cgtcgatcat cttcttgacc atctgaccgc ctacagagcc
     6961 gacctcacga gctgtaagat cgccgttgta gccctgcttg aggtttacgc caacttcgtt
     7021 agctgcttcc atcttgaact tgttgagtgt ttctctgcct tcctgagttg tgataccttt
     7081 cattttcgta tctccttttc atttgttttt cgggtttgca ataatattat atgtctgcat
     7141 gatttgatta taagaggtaa tttatagaac gaaatggggc ggtttttgga atgaaaaaaa
     7201 tcaaaagcta aaattagtat gattttaaac ggagcaaaat tcaaaaaaaa tcatttacat
     7261 ataaattata cttcttttgg aaattgtgtg attttgtata gtcacagttt tcaattgtca
     7321 aaataccact ggacacagtg attacatgcc atgcactgct gctgccatat ttcagcacat
     7381 aaccgttgag atcaagggtg acggtgcggt tgaccactaa tgtggtgtaa atggtaatgt
     7441 ctgacgtcag tttgactgtt ccgtatgaag cttctctgat tgctgaaacc agttccgccg
     7501 ctgtggaaac ttccgccgtc ccactcgctg ccatattatc ctcggcaaat acacttgtcg
     7561 acacaaggca aagaaccata caaagggtga gcaggatgct gagtattcgt tttttcattt
     7621 cttgctacct ccgttttgta tatgtcggct caatcaagag caatgattgt aatagacagc
     7681 accgttcagc tgtgtgccgg gggtcaactg gataaattca tattgcagca caatatcact
     7741 gccggcattg tagatgcggt tgaatgcaaa cggcatccac actcgtgaat tatctgcatc
     7801 gttcatatac ctttcgatca atgtatcatt aagaatgcct tgtctgcctt tcaatactac
     7861 cgagtaccat ccacccatgt cgtgagaaat gcagaccgaa ccgctgaatg aaatactgta
     7921 tgtatgtccg gctttcagcg ttacggttct attggctcga tcccatacaa aaagtcctcc
     7981 ggaattgcaa tcttcaaaca actgaagcgg ctgattgaat gaagcgccgg tgccgccgtt
     8041 tgaggcgtga aaataccccg aacagctgcc gttctgaccg gcatcgccct tgtcgccttt
     8101 atctcctttg tcgccctttt ctccggcatc acccttgtca cctttatctc ccttgtcacc
     8161 cttttctccg tctgtgccgt caatgcctgc ggcttttacg ccggtgtcgg tttctccaat
     8221 ccaccagttg ccgttctcgc cgatgaacgg ggtgtttccg tccacaccgt cagagccgtt
     8281 ttgtccgttt gttccgtcct tgccgtcggc tccgtctttt ccgtttatac catccttgcc
     8341 gtcaagacct ttcaaaaccg aaagatcgta caggttcagc catatttcat cgccgacata
     8401 gcgccactgc agggtgtttt cgcttacacg aaactccggt gtctttccgt ttgctccgtc
     8461 tgcacctttc tgcccggtgg gacctgtgat gtcggctatc gccacaaggt cctgccactc
     8521 atcgccctcg taacgccact gtatgtgggt ttcagctcgc tgcacctcta tgcttttggc
     8581 gttaatgccg tcagtgccgt tgataccgtc tatgccgttg gctccgtctt taccattggc
     8641 accgtcaatt ccatccttgc cgttttttcc atcggctccg tctttaccgt caacgccgtt
     8701 aatgccgtct ttaccatcaa ctccgttaat gccgtctttt ccgtttgtac cgtttattcc
     8761 gttcttaccg tcggcaccat tagagccgtc tttaccgtct gtgccgttct ttccgttttc
     8821 tccggcagcg ccccgcagct cggtaagcgc cacaaggtcg tgccagtcgg cttcgtcgct
     8881 gtatttccat tgaatcgccg tgccgttgtt tcgtacctcc ggcagtgcgt cgcctgtttt
     8941 tgctgccttg ctccatccta ttgcaagcac gctgcaaagc accaaaagag cggtgcaaag
     9001 cgccaaaagt gtacctgtga gtagtttttt tcctgttttc atcttttttc ctccgttcgg
     9061 aattgtttat cacggcggag ctttgcaccg ctgcccacgc cgtaaatgag cggcggtcga
     9121 taggcttatg cttgatttgc tttgtactta tccacaaaca gggcgacccc ctgttttaag
     9181 acctccatct gggttttcag ataatattca tcctcaaaca ttgcataatg aacgcaggcg
     9241 cggacgaata taaagatcag cggcgtgatg accgtatatg gaatgcctat cttcggctcc
     9301 agccgcttgg cgtactcggt atagcgttcg ttcacgccct caaagaattt cttgccgtgc
     9361 tcgatatact tcgggtgcgt atatatctga tacatcaggc gatatttttt tccgtgcttt
     9421 tttgctgtcc aatacgggat ctccttcaca aaacggacaa catcctcggg gtcggtgggt
     9481 gccatctcca taaagtcgtc ctcaaccttt tccatacagt atgccgttga ctcaatgatc
     9541 agttcatcaa ggtttttaaa atacgaatac aaatttgctt tcgtgcatcc gcacgcatcg
     9601 gctagtgctt taattcctgt gcctgtgagc ccgttttccg catagcactc gaagcacttt
     9661 tccattagtt cacgtttccg ctcattgtgc tgttgctcag ttgttttcat gttgcttcgc
     9721 tcctttgtat gtaccgaccg tttgtttatg attagtataa cacagatttg taacaaagtc
     9781 aagaattgtt caaaaaatga agaaaataaa gtttcaacat gatcttataa aaaagtcctt
     9841 acagcagaaa gaaaatagcg gcttacctca acgatcaaaa ccgtactaat aaatatgtcc
     9901 ataagactta tggaaaggtg ggataggctt attgactttc catgttctca tatgatataa
     9961 taaattcata ttaacacaac atttctgttc agggggtata tcaaatgaaa aagcaaaatg
    10021 cagggcttat cgctctgagt atcgttttta ttcttatggg tggtttctct actatctata
    10081 atctcagcaa gtatgtttca aacagggttt tcatgtcaag tgctgagaaa accacaggct
    10141 atgtgaagtg gatagatcat gagttgaaaa cttggggaca taacaaaaag gcaagcaaca
    10201 aagctgaaac atattacata actgtcgctt ttgatgttgg cggcaaggag tacacaagag
    10261 agatcttcac aggcacatat tatgtgagtc agaacgaaaa agtgaacgtg tattacaaca
    10321 cagataaaaa cggtgagcct gataaggtcg tgaccgatat tgataatgat aaaagaccgt
    10381 atcttacttg gccgctgata cttttgcttg gagttgtgct acttgtgctt actaagaaaa
    10441 atattatatc ggtggggaga aagagataaa aaagtaatca ttaaaatagt aaccaaaaga
    10501 aactttgtgc agctaaataa cagaaaagca gaaaggagat ctttatgaag gtgatctata
    10561 aatgtgccgc cattttgacg gcggcgatat tgtcagtgca gtttccttgc gcagcttttg
    10621 cggatgcgga gcatggcgga gataatgaca gcccgaaatt caagatgggc aggataattt
    10681 ttactgataa tggttatggc tacgaaaagg aaggtctgga aaaatcggag atcgctgatg
    10741 aagatctggc cgatacggac gacgggggtt caccttatgc acagaaggca acggcttcgc
    10801 tgcctgcgaa atatgacccc agatctaaga atacgctgac gactatcaaa aatcagttcc
    10861 agacaggcac ttgctgggcg tttgcggcac tttcctgcac tgagagcaat cttatccaaa
    10921 aggggcttga aaatcaaaac gtgcagtttt ctgtgccgag ccttgccctc tctacatatg
    10981 ctaacggcgg atttgacgag tcaatatggg attgcggcgg aacgtggatg gattcgtccc
    11041 ttgcaatggc ggctcataga ggactttgct atgatgaata tgagcctttc gtgtccgacg
    11101 gaacggaagc cactatcgta tctgaggaga aaaagggcgt ttgcgaatat cagcttaatt
    11161 acgcttccac tatcacaggc ggacgaaacg ccattaaaaa caagataaaa gagcttggcg
    11221 gagtaatggt gagctattat gcagacgacc tgtatatgac ctttgataac aaaagctatt
    11281 acgatccgga aactacaaaa tatagtggaa taaatcatgc cgtaagcgta gtgggttggg
    11341 acgataatta cagcaagtca aattttcttg caggctatca gccaaagaaa aacggggcat
    11401 ggcttgtgaa aggctcttgg ggagtatcca gtggcacaga cggttattac tggatatcct
    11461 atgaagaggc agaaatgacc gattgggtgg cgttcgacct cactgaaagc tgtgataata
    11521 attaccatta tcctgctaat aaggataatt ctatatttta tgttagcaat gaaaacaaaa
    11581 tgagcggtgc tgatg
//
LOCUS       DAWEMR010000075        11545 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2359_length_11545_cov_11.534813,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000075 DAWEMR010000000
VERSION     DAWEMR010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11545)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2359_length_11545_cov_11.534813"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3345 a         3008 c         2256 g         2936 t
ORIGIN      
        1 tttacttttt atatagcata tcctgtccta aggaaaaata ttgcacaatg tgtttatttg
       61 tgctataatg agtatatatg actgcaccgc acgcttcttg ttcggtgttc cttattaaaa
      121 aaattattgt cggaggggta aaagtggata atatcgtcac tgtcggcgga cacataaacg
      181 catctataaa ctttgccatg cagcaaaact atgtccctgt cataagaagc cttgtagtaa
      241 ataacaactc agaggaagtc cttgagaata tcagccttaa aataaccttt gagcctgagt
      301 ttgcaaagga atttacatat tatatcggct ctatccccgc aaaaagttct gccgaaatat
      361 cccccgtgag gatatctacc aacacagatc ttcttttctc tcttacagaa aaaatggtgg
      421 gcaatatcac cattgaggtg cttcaaaatg gcgaaaatat cttcacctat cagaacacta
      481 tagagcttct cgcctgcgat cagtggagcg gtttgaacat aatgcctgag atgatagccg
      541 catttgttac gccaaatcac cctgcgctct ccccagttat ccatgacgct tccacgttcc
      601 tgaaaaagtg gaaaggcgac ccctctttca caggctatca gaccaacaac ccaaacaacg
      661 tgaagcttca aatggcagca atttttgccg cccttgtcca gcagaaaata gtctacaacg
      721 atccccctgc aagctatgaa gtcataggtc agaggatacg cctgccgcat aaggtcttgg
      781 agcaaaaaat gggcacttgt cttgaccttg cggtgcttta tgccgcctgc cttgaagcgg
      841 tgggacttca ccctctgctg tttttcatga caggtcacgc attttgcggc tgttggcttg
      901 aaaacgaaac tttcgcagat tgttgcgttg acgatgtttc cgctattgaa aagcgcattg
      961 cagaaaatgc cgaagaaatg ctccttgtgg agtgtacaga ctttgttgac agcaacgttc
     1021 acgatatcga gcgtttcgac catgccatga aacacggcaa agaccatata agcaacatgg
     1081 aatttcaatg cgtcatagac attatccgca cccgtggaag tggcataaga cctatcccac
     1141 tgcgtcccga gcaaaccttt tcaggtatgc agcttgcaga agaatccgac aagccaaaag
     1201 agattctcgc cccctccgag cttaacagct ctcttcttgg caaagtggca gagggcaacg
     1261 ataagcccgt cacaaaaatg cgtatatggg aaagaaagct ccttgatttc agcctgcgaa
     1321 attctctttt gaacttccgt gttacaaaga acaccatgca gcttatgacc gcagaccttg
     1381 gaaagcttga ggacgagctt gcaagtggaa gtgatttccg tatcatggaa atacctaccg
     1441 agtggacagt ttccactcgt gacgcaaaaa tattcgctat cgagaacgaa aaggaccttg
     1501 tgacaaatat cgccgagaac gagttcaaaa acaaccgcat aagaactttc ctcagcgaaa
     1561 ctgaccttga ctctgccctt aaaagcctct accgctccgc aaaggtgagc atggaggaaa
     1621 acggcagcaa cacccttttc ctcgctctgg gacttctccg ctggtatgaa agcgaccttt
     1681 ccgaaaagcc acgctatgca cctctcgtgc tcataccgat agatatcgtc cgcaacacac
     1741 gcaataaggg ctatataata aggagcagac aggaagaaac tcagataaac gtcacacttc
     1801 ttgaatatct ccgccaagac catggcatca gtatcacagg tcttgaccct ctcccacttg
     1861 acgaacatgg catagacctg ccacttgttt tcaatactat ccgtcaggcg gtcatgggca
     1921 aaaagcgttg gaacatcgag gaatatgcct ttatcggtct gttctcgttc agccagttcg
     1981 taatgtggaa cgaccttcgc aaccgctcag aggagataag ccaaaacaag gtggtgtcca
     2041 gccttatggc tggttctctc acctatgccc ccgaggatat aagcataacc cctgagaata
     2101 ttgattccga ccttgacctt gaaaacatgg cagtgccaat gagcgcagac tcctcacagc
     2161 ttgccgccat tgccgcagca ggaagcggac aaagctttgt cctccacggt cctccgggaa
     2221 caggaaaatc tcagactatc accaacatga tagcaaatgc cctctacaat gacaagtctg
     2281 tgctgttcgt tgcagaaaaa atggcagccc tcaacgtggt gcagaaacgt cttgcaaacc
     2341 tagggcttga cccgttctgc cttgaactcc actccaataa aacaaacaag accactgtcc
     2401 ttgcagagct tgacaaagcc cttgaagtgg gcaaaataaa atctcccgag gagcattcag
     2461 ccgaagccgc aaggctcaaa gctcagaaag cccagctcaa cgataccata accgcacttc
     2521 acgaaaagcg taactgtggt gtgacccttt atgaagctat atcagcctat gagcgcagta
     2581 tatccgaaaa agacaaaata tcagtttcaa aagatatctt cacaaacctg gcaaaagaaa
     2641 ctctcgaccg cttcagcaac cttgtgcata gatattcaat agccatagcc gaaacaggcg
     2701 actttgccaa cttcccactt aaagatatcg gcattacatc atattctatg gaacagcgtg
     2761 acaagttcca cactgaagcc gccgaccttg cccaaaaagc caccgcttcc tctcagtgcg
     2821 cacaaactct cgcacaggct tatagctaca gcggcggcct tgacaagaca gcagtaaaaa
     2881 tgctttccca catatacaaa gcttccaccg ctgacggtga acttcttgac ggacttctct
     2941 gcgcacctca ctatgatatc accctgcaaa agatcaaaga tactcttgcc gtaggaaaag
     3001 agtataccgc tctctgcacc gatatcctca gccattttga aaaaagcatt tttgaccttc
     3061 ctgcgtcaga atcgaaaatg ctctataagc aggcagagca aagctggttt ttgccaaaag
     3121 ccataaagct tggaaagctc acaaagggaa taaagctcca cgccaaagac cctgcttccg
     3181 ttacaaaagc aaaccttggt gaaattctcg ataagctttg caccataagc gaaaagaaag
     3241 acttcctgcg aagcctgccg tcagatgtaa cagcgcttct cacaggtatt tacatgaacg
     3301 agcagaccga ttggaatgtc cttgaaaaag ccgtttcaaa aaccgacagc attatgaatg
     3361 ccattaagca gaaagcgggt gctgaccttg cacaaatggc tcagactatc ggaaaagcaa
     3421 actgcacttc tgaaggacag gctctctcct cgttccttga aaagctttca gcctttgagg
     3481 acaacttctc agccaacatg accgccatag aagcaagtgc agattggctt ggcttatctg
     3541 cagaaaagct taatacctat gccgaaaacg ccgataagct tagatacgcc gcccagttca
     3601 acagcgttga caaagagctt tcagacaacg gactttcagg tgtcagcgaa agctaccgca
     3661 caggcaatgt tgacagcgaa aacgtagaaa aagcattctc ctgcacgcta aactatcagc
     3721 ttgcacttat gataataaac agcgataaac gtcttagcca gttcagcagt aacagtgcaa
     3781 acgaccttat cacacagttc aaagacactc tcgataaatt ctcacgcctg actatacagg
     3841 agcttttggc tcgtctttcc gcaaaaatac ctgcccaagg ctcagcctgc gcttccacct
     3901 ctgaaatggg catactcaaa cgtgccataa agagcaacgg cagaatgatg tcacttagaa
     3961 gccttttcga taaaatacca aatcttctcc gcaagctctg cccttgtatg ctcatgagcc
     4021 ccatatcagt tgcacagtat atagacccgt cattcccgaa attcgacctt gttatctttg
     4081 acgaagcttc acagctgcca acagccgaag ccgcaggcac tatcgcaagg ggcgagaacg
     4141 ttgttattgt gggcgaccct aaacagctcc caccaacaaa cttcttctcc tcaaatcgga
     4201 tagacgaaga aaacagcgaa aaagaagacc ttgaaagcct ccttgacgat tgccttgcta
     4261 tctccatgcc gcagcagtat ctcaaatggc attatcgttc acgccacgag agccttattg
     4321 cctacagcaa catgaaatac tatgacaata agcttctcac attcccgtcc cataacgacc
     4381 tcatttcaaa ggtgagcatt atccaccccg agggtcacta tgacaagggc agaacaaagc
     4441 agaacaaggc ggaagcaaga gccgttgttg acgagataat aagacgaatg agcgatgaaa
     4501 agctccgcaa tgacagcata ggtgttgtaa cattcagctc cgttcagcaa aatctcattg
     4561 acgatatgct ctgcgaagag tgggcaaacc accctgagct tgaagagctt gacagaaagt
     4621 caccagagcc ggtcttcatt aaaaaccttg aaaacgtcca gggcgacgaa cgtgacgtta
     4681 tactcttctc cgtcggctat ggtcctgacg aaaagggaca agtttcaatg aacttcggcc
     4741 ctctcaaccg tgacggcggc tggcgaagac ttaatgtagc aatatcccgt gcaagaaaag
     4801 ccatgatagt ctactctgtc ctccgtcctg agcagataga cctttcaaga acacgttccg
     4861 agggcgttgc aggacttaaa ggcttcctgg agtttgccga gcgtggaaag cttgccgtaa
     4921 cagcccacag caccacaaaa agcacaagcg acagcactgt aacagagtgt atcgcaaaag
     4981 ccataaaaga gcttggctat ggggtgaaat gcaacatcgg aagctccgag ttcaaagttg
     5041 atatcggcat aatcgaccct gacaatgaaa aggagtatct cttgggcata ctccttgacg
     5101 gtgaaaacac tctccacagc tcaactgcac aggatagatt tatcctacag ccaagcgtgc
     5161 ttaacggtct tggctggaat atcctgagag tgtggacgct cgattggttt gacgataagg
     5221 accgagtgct taaaaacata aaagccgcca tagacagcgc accaaagcat gaagccgaaa
     5281 ccgctccccc ctcaaagcct gccgtctatt ccacatcgca gtttgaaaaa gaagaagctt
     5341 ccgccctcac atcagccttt gcacagccct atgtgaccgc agatattggc ataatgggta
     5401 catctgatga cttttataag cccgaaaaca agcagaaaat aagggaaaca gccgaaaaga
     5461 tgatcgctgt cgaagcacca ataagtgaga agcttttcat gagaaaagtt ttcaccgcat
     5521 ggggcataac acgaagcggc tcaagagtgg aaagcatctt tgccgatgcc gcacaaggcg
     5581 tttcaccatt cgccacagcc gatgaaaaca gagttttcct ttggaaagag gggcaatccc
     5641 ctgaaagcta ttctatctat cgcacaagcg acgataccaa caagcgaagc atggaggata
     5701 tcccctccga ggagataata aacgccgtga aagaagtgct ttgccagcag ataagccttt
     5761 cagaaaccga cctcgtaaga gaaacagcca agaaattcgg ctacacaaga gctgtgggca
     5821 taacggagag cgtaataagc tacaccctga aaaaagctct caccgcaggt cttatcaaaa
     5881 aatcagaaag cggaaacatc tccgcaaaat aatacgcaaa caaaccaaaa ccaccgccga
     5941 gagctttcct cccagcggtg gtttctattc atatcagtat ttccatttct ccgagcaaag
     6001 ctgcatctca accatgtgct tgtagatacc gttagtcttt ttaagcgcct caggcgagcc
     6061 tgtctcagcc accttgccgt ctttaagcac aactatcttg tccacaccgt caacagttct
     6121 cattctgtgt gctattataa gcacagtctt gttctgtata agctttgcaa tagactcctg
     6181 tataagcgac tcgttgtcaa cgtccagaga agccgtagcc tcgtccataa gaatgatagg
     6241 agcgtccttt agaaatgccc tcgctatgga tatacgctgt ctttcaccgc ccgaaagctc
     6301 cgagccgttc tcgcctatca tgctgttcca cttgtcaggc atcttctcaa caaaatcctc
     6361 gcagtgtgca agccttgccg ccgccataac ctcctcgtcc gttgcgtcct ttctacctat
     6421 cctgatattc tccataacag tgttgttaaa cagcgttaca tcttggaaaa cgatagagta
     6481 aagtgacaaa agcttctcag ggtcaacgtc tgcaacattc attccgccaa cagttatctt
     6541 gcccttgtca ttatcccaaa atcttgccgc aagccttgca acagtggtct ttcctccgcc
     6601 agacggaccg ataagcgctg ttatctcccc ctgctttgca gtgaagctta catcagaaag
     6661 cacctgctct tcactcttgt atgagaaagc aacatggtcg aatgtgatat cataaccctt
     6721 gttgtcaagc ttatcactgc ccgactgctc ctcgtgcgaa agtatctcgt ccatacgatt
     6781 gcagtttgcg tcaaggctta ttatagcagc caaattctga agcgctatct gaagcggctc
     6841 atacattctt gttaccacca gcaggaacat aaagaacgtc agcaccgtta tctcaccctt
     6901 tacaagaagc gagcttccca caacagccac agttccaata ccgagcttta gtatcatctg
     6961 cgctgagcat acaaataccg catttgcaaa ctccgccacg atagcgtgct tttccacagc
     7021 ctttatcttc ttttcaagcc cctcagagta tttctctgtc atgttgtatg aacgcaggtc
     7081 acggagagtt tcaagcccct cctgaatgcc gtcaatgcaa tctatacgat atttcatcgt
     7141 cttttcgtgt atccttgaca taaccttctt tgaagaaata actatcataa atgctaccgg
     7201 cattacccaa actgaagcaa gagccattct ccagtcaaag aaaaacaagc ttataacaat
     7261 gatagtagtg gagacgatac cgcccacaag ctcaggtatc caatgcgaag agcctgtttc
     7321 tatcattgca cagtccgtca tgatagtgtt cgtaaggtcg gcaagatctt tcttgccaaa
     7381 gtatgaaagg ggtatcttcc tgagcttttc cgcaagcgtc cttcgtctta caccgctttc
     7441 aacatatgta gagtaaaacg tggacttata ctgaaacagc gttgtaaggg caataagcaa
     7501 aagcactccc actatcgccc cgacaaaaag ccagaactta tctcttggaa gctctccctc
     7561 aagaagatat gaggaaagca catataaaag ccccacaggg agcataagca caaggttagc
     7621 aagcgtaaca gcaataaacg cccttatcat gccttttgca cccttgtctg aaagggcata
     7681 tttgtgtttt aatttttccg tgaagctcat aatcaaacac ctgcctttct gacctgccag
     7741 cttacagacc tgttgtattc gttccacata cggctgtaga tcccgccctg ctctataagt
     7801 tcctcatgct ttccgctctc tgctatcctg ccctcagaaa gaacgtaaat gcggtctgca
     7861 tttgttaccg ttgaaagcct gtgtgctatc attataactg tcttgtcttt tgaaagcctc
     7921 tcaaaagcct gctgaacaga cctctcgttg tcagggtcgg caaaagccgt cgcctcgtca
     7981 agtataagca caggcgcatt tttcaaaaac gcccttgcaa tagaaagcct ctgacactcg
     8041 ccccccgaaa cgtatatgcc ctttgagcct atcatagtat caacaccctc agggaacttc
     8101 tcgattatat ccatgcactg tgcgtcacga agcgcctgca taaccgcatc gtccgtggcg
     8161 tcaggtctgc ccattcgcac gttgtcgaga atactcattt taagaagctt gctgtcctgg
     8221 aaaacatatg aaacatactt cataagctcc tctgacggaa tgtttctcac gtctgcccct
     8281 cctatcttta tagagccgct ctttacgtcc cagaaacgtg cgataaggga acaaagggtg
     8341 gtctttcctc cccctgaagg acctactacc gcaatatgct ctcctgcctt tatattcatc
     8401 gttattccgt caacagcgtt gctctctgca ttgtcataag caaacaccgc attgtcaagc
     8461 tggatagatg agtcctttgg agtttcaccg ctctttgtct cagcaagcgg ctcagtttca
     8521 agaatagaat acattctgtt cacagcgtcg tccaccaaca tctgtgactc tcctgcatat
     8581 gctatcttca taagcgttac agtaagaata gaagttatta tcacataaaa gaaaagatta
     8641 agaacaaact cgtccgtaac tccatgggaa gtgagtttga aagccgcaat tataagcgcc
     8701 gcaaaaatgc cgttcgccgc agtggtgaag catatcatcg gaagcatcat gttctttgtg
     8761 tatccaagag tccacttttc atattcgtca atagcaagct tgaaacgctt gaaagaaaat
     8821 accgtctgac caaaggtctt tacaacaggt attccacgaa catactccac cgcctcagag
     8881 gacatttttt caagtgcatt ctgatactgc ttcatatcct ccgccatttt aggacccatc
     8941 ataagcgtgc ccataagcac aaatgcgatg aacgcaggga taaggctaat aagtccaagc
     9001 cgccagtcga atatgaacat cataacaagc agacccacag gggtcgccat tgaaacagcc
     9061 ttgtcaggaa aattgtgggc aagataggtt tccgttgacg cacttaaatc cataactatc
     9121 tttcgtatct ttcctgttcc ctcagcttca acataaccaa gcggaagctt cataatatgc
     9181 ttcatcatgc tcactctcat gttagcctgc accctgaacg ctgccttgtg agaacacatt
     9241 agcgcagcga tatatataac cattccaaga agtgcaaagc ccacagcctc ccagccgtat
     9301 cggcttatgt tctccgcctt tgaaaaatca ggtcttaccc tgagtatctc ttttatgatg
     9361 cgccaaacat cataaaacgg tatcagtgct atccatgcac ttattacagc aagaacagca
     9421 gaagcgtagg aataatactt atgtccgcct gcatagtgca taagaactgc aaaggaactt
     9481 ttctttttct ctttcttttt cacaacaata cctcctgttt gtttataaag ttaggtctaa
     9541 ctaacttatt gttatttaaa aagcaacacc ctataagggg ttgctgaaaa tgttaaaaaa
     9601 gtcaaatgat tttgacatct tttctgtagg ggagcggcgc aatttctgct ttgcagaaat
     9661 acgacgtccc gctccctgct caaagttcta ctaaaaaccc gaacaacacc aagaccctac
     9721 aagcccaggt cctcataatt ctgccccaga actttacccc aacctgcgaa gcagaactct
     9781 cttatgcttt tcataaagat ctgcgcctca tcataagaaa gatcatgaga gaccacctcg
     9841 taaatataag accagcctgt tcggcagata acgtgtataa agaagtcgct caccatgttt
     9901 tcacgccttg aaaactgctt tacaaactcc ctgtaatact tttcctctgt ctcagcaagc
     9961 tcgtcaaaat aatgctcata ctccgttcct gccgagtggc agaagataag cttgaaaacg
    10021 tcaaagtttt cataaatata tttcagcaca agctcagttc cctcattgcc ctgacttgtc
    10081 gcctccgcag ggtcgccgct ctcccttgca gcggttatgc tttcaagata caactccata
    10141 agtccgtctg cagccggctt taccaccgcc gaaaaaagtc cagccttgtc cccaaagcgt
    10201 gtatatatag accccgtact tactccgctc tcagaagaga tcctgcgaag atttgcattt
    10261 tgaaagccgt gttcaagaaa ttcttttttt gcactctcta taagagtttc tgtaactcct
    10321 gctgtttccg ttctcatttt gcacctgcct gaaaataacc atgttataat aacagtgtta
    10381 ttataacatg gttatttaaa cttgtcaagg gggaatatca tatttttttc aaatttcggt
    10441 atacatccct ctatcacctt tgtgatatgc tctatgtcat cctcacagcc gccctttatc
    10501 cattcctcta ttatccctgt gatacccttt agataaaacg ccataagata atgcctctcg
    10561 tccaaaggga caccaaaccg ctccataaca gggtcaaaaa tgtgtttgaa catacgctta
    10621 tatgtagtgt caacaccaaa taccgcaggg tgttccgttg ccgcacgata caattgacga
    10681 ttttccttta taaactgcaa atacggcttc agatattcag gctttatcca cataagctcc
    10741 tcatatgaac agctttctat cttaggaata acgctcagac tgcccttgaa acttgccaga
    10801 aattttttct gcatatattc aagactttcc tccagcagat cccctaccgt ttcataatga
    10861 agataaaagg tggagcggtt cacccccgcc ttttcgcata tttcctttac cgtgataaaa
    10921 gaaaaatcct ttctttgcaa aagatcaaga aacgccttgt ccattttctc tgccgtgttg
    10981 aaatatttgc tttctgactt gttcaagttc ttcacccctc tgtttttatc ttcctgctgt
    11041 gcacaagcat atacaccgca atgactatca ccgccaagct cagttccttt ttcataaatt
    11101 acttgtcact tatctctttt gcaagctgga ttatctcaaa agcatatttc tgctttccct
    11161 tttcaagaag ctttttgtat atcggcagat taactcccat gcctatcatg ccgataatgc
    11221 caatgataat tcctgcaacg aaagaagccg ttgagccgct tccgataact ctcattccaa
    11281 ggcacattcc aatgccaaaa accagtgccg aaagtattcc cagcgagtaa gtaaaaatgt
    11341 tcgcaggaag ctttgccttt ttatcgagct tgcgaagtgc caccaccttt gatgtgtcct
    11401 ttggtgcata ttcatttgcc aactgttctg cgtagatctt atctgtgttc atttttcaca
    11461 gcctcctttg tattttatgt ctatattata cataactcca aaagaaaact gaacaacatc
    11521 tccgcagctt tgtgtcgatt ttaat
//
LOCUS       DAWEMR010000076        11524 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2366_length_11524_cov_11.229052,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000076 DAWEMR010000000
VERSION     DAWEMR010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11524)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2366_length_11524_cov_11.229052"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3164 a         2176 c         2962 g         3222 t
ORIGIN      
        1 tcatggaact ttacgctgtc aaaggtgaga tactcgtctt ctttttcggg ataggcgacg
       61 caggcataga ggtttgttat atgctgtgcc gcaaggagct tttcaagggc ggagtagagt
      121 tttgcaccta tgcctttgcg gtgaagtcct ttttttacat aaactgtgac ttcgaccgac
      181 caatcatagg cggctctgtt tttgaaaaca cctgcatagg cgtagcctgc tattttgccg
      241 ttttctactg cggctatgta tggataattt gcggatatgc ggcggatcca gtcggaaaac
      301 tcctccacag aggggacgtc atactcgaat gttatggcgg tgtcttcatc gtagggacgg
      361 tatatttcaa gaagttcgtg agcgtccttc tcggtaactg ttcttattat catggcgttc
      421 tccttatagt accattaata ctgtgcccgc agtgattagc accgcaccta tggctgatct
      481 tactgtgaag tcctcgtgga gaaatatcac tgcaaggata agtgtgatga caacgctgag
      541 cttgtctatg ggggcgacct ttgaaacgct gcccatttgg atagccttat aatagcatag
      601 ccacgaagca cctgttgcaa gtcctgaaag gataaggaat acccagcttt ttctgcctat
      661 agagcctatg ccgctttgtg tgtgggtgat gaacaccatg agccatgcca tagcaagcac
      721 aacgcaggta cgcagtgcgg tggcaagatt tgagtttact ccttcaatgc cgacctttgc
      781 aaggacagag gtgagcgctg cgaaaacagc ggagagaagc gcaaacacga accacataat
      841 accactttct ttcgtgaaaa attgtttgct catgccttat tatagcacct aaaggtgctt
      901 tttgcaatag aatgagcggc aggacgtgat attttacatc agacctgcca cttatgctta
      961 gttatttgcg gcaaatattt ttattgttcg tgaaagtgct gttacctgct gaggggtact
     1021 gaatgcagag ggtgagaaac gcactgtgcc ttgctctatt gtgccgaggg tggtatgggt
     1081 gacggcggca cactgaagtc ctccacggag ggcgaatcct ttttcgctga gataggaggc
     1141 gatctcagcg gaagggatat caccgatgtt aaatgaaagt atggggactt tcttctggtc
     1201 tgcttcatag aactttattt tgtctatgtc tgaaagctgt tcgtagagct gtcggcagag
     1261 cttttcttcg tgggctttta ttacggcagg ggtcttttgg cggacaaacg ttagcgcctc
     1321 gccaagaccg attatgccca ctgtgttaat tgtgccgctt tcaagctttt cggggaggaa
     1381 aggagtctgt tcaagcaggg cggaggtcgc acctgtgcca ccctcgatta tggtagacag
     1441 agaatactcg ccgtctgaga taagaagtcc tgtgcctgtt ggtccgtaga gggctttgtg
     1501 acctgcggtg cagagaaagt tgaagccgtc tgagagagga agctccaaaa gcccgcaggc
     1561 ttgtgcgccg tcggctataa agcatatgcc gtgggctttg cagagtgcgg ctaattcacg
     1621 gtatggcatg agcctgcctg tgacgtttga agccactgta cagcagatac acttagtttc
     1681 gggggttata aggcggcgga agttttctac tgtttcgctg tctgtttccc ctgttcgggc
     1741 aatagaatat ttcacgcctc ttgtttttga atgggcatag acgggtcttg ctactgcgtt
     1801 atgctcgtgg tctgagatta tcatatgacc gccgtactgc atgacacctt ttatcgccat
     1861 gttaagagca tgggtacagt tggcggtgaa agccacgttc tcggtctgtg ctccaaacat
     1921 ttctgccgcc tgctgtctga cggtgaatat cttttcagac actcgcatgg aaagcttgtg
     1981 accgcttctg cccgggttgc caccatagcg ttctactgcc tgagcaacgg cttttgctac
     2041 ggcagggggc ttgggatagg tggtggctga gttatcaaag tttattatgc ttcgggtgtt
     2101 tatttttctc acctcttttg ctcgaatatc acgtttctga atggtatgcg ctctttttca
     2161 agtatggcgg ttatttcttc tgtgggtgca gtgacggcta tggagtagcc gcagcctgtg
     2221 ccaaggtcct tcggggtgcg ctgtatttcg cagtagtatt ttaaactgtt cagcaagttt
     2281 tttgctttca ttgcataggt tatggaagtc atggctataa gactcttttt catttttgct
     2341 gctcctttat gctgtacttg tccgtatacg cttttacgaa tacacttcag tatatgccgt
     2401 tcactgtttg agggtgaacg ctgaaagttt tgtgaaaggt aggtgaaatt tgataaaagt
     2461 taatagtcag tttaaatttc gggtgtacta tttataatat aaaaagaaaa gaggtaaaag
     2521 cttatgaacg gatttatgta taaggtagcg agattttttc agggcagata tggtatagac
     2581 aagctttttt acggcatttg tggagtggct gtggtgctta gtgcggcgaa tttgtttgta
     2641 aggtcgctta ttctacagtt tatggtatac gctgtgctgg tgctggcggt ggtgagggcg
     2701 atgtcgagaa actgccagaa gcgatacgag gagaacatga agtttgagag ggctttcggt
     2761 ggaattggca ggaaattttc attctggaag agaaagttcg ctgacagaaa gacccatgtt
     2821 tatataaagt gcggtgtatg caaaaagacg ttaaggcttc caaaggtaaa gggtgaacat
     2881 actgtgaaat gccctaactg tgcaaataag ttcaaggtga agatctgacc tgttgcagaa
     2941 tttgcggaaa tgtgctatac tatacaatat agtatgatac tgtaggggtg caaggacaat
     3001 gggaatgggc cgtcgaggac gccagcccct acggaagtgt gtcaggtacg attttttgcg
     3061 aaaagggagc ggcaggaatg cagaaagtaa attatcagaa aatgctggat ggcattataa
     3121 aaaaacatga ggaaaagggc gaagtgccga ctttgctttt gcatagctgt tgtgcgccgt
     3181 gttcttctta tgtgcttgaa tatctgtcgc agtattttaa gataactgtg ttttactaca
     3241 atccgaacat ttatccccgt gaggagtacg ataagcgtgt ggcggagctt caaaggctta
     3301 ttgcacaaat gcctatgaaa aatcctgtta cgttcgtgga gcgtgggtat gacgagggtg
     3361 agttttttaa ggctgttaag ggctttgagg atatacctga gggcggggag agatgctttc
     3421 gatgttacag gctcaggctt gaacggacgg ctcagcttgc caaggagctt ggtgcggatt
     3481 atttcaccac aacgctgtct atttcaccat acaagaatgc tcagaagctc aacgagatat
     3541 cggaggagct tggggagata tatgatgtaa aggctcttcc tgcggatttc aagaagcgtg
     3601 agggctacaa gcgttccatt cagctttcag cagaatacgg gctttacagg caggattact
     3661 gcggctgtat cttctcaaaa agagagcgtg aaagactgaa tacgattaca acaaaatctg
     3721 aataaatata aaaatattaa cgttatgtag aatgcttgtg catactatgg caacacatct
     3781 ggttatatat agcaaaaata atatacagat ttgtgaaaaa tggctaaaga tatgtggtta
     3841 atttgtgaaa aacgtttatt gaaatacttg taaatgtgtg ttacaataaa ggcaaggtgt
     3901 atgttgagga gatatcgctc aatatgtatc taaaaaatta ttaataaaaa tggaggaaaa
     3961 acaaatgaaa atctctaaga ttttggctgg tatgtcagct atggctctcg cagcttcaat
     4021 gatgactatg ggcgcttccg cagaaatttc aaatgcaaat ttcaatgacg gcaaggacta
     4081 ctgctttgac gtatttgcag cagtaggtgc tgaaaaggca gctactatct caagcgtaag
     4141 agctaagctc tctttcaaga acattgatga tcttaaggct aacggcgcag gcggcggatt
     4201 tatcacaagc agcgacggaa ataactggaa gtcaacagag ttcggtaacg acggtgctga
     4261 caagccaatc acattcgtag ttgacgaagc ttcttcaaca gctacaatgg aaaagactct
     4321 tactgatgct gacaagttca caggtagtgg cgaggacgct ctgtcatatg ctcaggtagt
     4381 tttccagagc tcgtggggcg aattttcagt tgacaacgta gcttgctacg atgctgctgg
     4441 cgaactcgtt tgctcagtaa caggcgacgg aattgacctt tacaaggctc ctgaaaaggg
     4501 cggaaacgaa gttgacctca gcgaatacaa gaagcagaca gaggttacac ttaacggtga
     4561 aggcagatct gtaactgaca gcggtaaggt aagaatcaac attaagcacc catgggtaga
     4621 tgatgcaaca ttcaaccttg ttgatgattg gagcgtattt gcaggtgctg acgctattgc
     4681 tgttacagtt gatatctcaa atgtaactga cgctttcaca gcttatccat gcttcacaaa
     4741 tggtattgag aacggtattg gttattggga agcttctgat aacaaccctg ctgttacagt
     4801 aaacgctgac ggcacataca catttatcgt taagttccct tcagctcttg aaatggacgc
     4861 aaataatatg ttctttgaca tcaacacaga cctcgctggt acagacggcg aagatcctgc
     4921 tgttaagatg gctgttaaga aggttgtagt tctcaacaag agtgaggctg agccaacaga
     4981 tccttctgag tcaacagctg atcctacaga ttcaacagat tctgacgatt caaatgatgt
     5041 tcacacagat tctgtagatt ctaagactaa ctctgctgct acatcttcta aggctgctaa
     5101 caacgcatct aacacaaacc caagcacagg cgctgctgct cttgctgctg taggtgttgc
     5161 acttgctggt gctgctgtag ttgctactaa gaagagaaag taatctgtaa ttcagataat
     5221 tcttacttag aataattaaa gacctgtctt tcgggacggg tctttttttg ttgctttttt
     5281 atcgaggtag tggtataatt gatctataaa ctgcggaggt gtttttatgc gtatagtttt
     5341 tgatgatatt aaagagagcc ttgcaccgaa tttcaagggc ggcgagaaaa gctttgcaag
     5401 cagggctttt tctgacggca agaacaagat gatgaaaggc aggcttgtgc cgggggcttc
     5461 tatcggtctg cacagacatg agggcaactg cgagatgata tatgttcttg agggcagcgg
     5521 caaaatgctt tatgacggcg gtgaggaaag actgtcggca ggagatttcc attactgccc
     5581 cgacggtcat gagcatagct ttatcaatga cggcgacagc gaccttgtgt ttattgcagt
     5641 tgtgccagct cagtagattt ttgcagcttt gtatctgtga aatagtgggt caaaatggct
     5701 ttgtagtctg agcctttttc tgccatggca tttgcaccgt actggctcat tcctacgaga
     5761 tgtccgaagc cgtgagaggt gaaagtgaat tttgtattgg caaaggtgaa ctcgaaacat
     5821 ggcgaggaga tatctaggat atcgcagaat gtgttgggtt ggatatcata gccacagacg
     5881 gtcaggttcg ttacatagcc tatgccgtct gtttttgttt tgagccaact atcagcattg
     5941 gcgaggggag tgaaatcaat atcggggaag cggtcaagaa gcttgtcttg aaattccttg
     6001 gcggtgagtg tctgggtaca ttctgtggtg acaaggtctt tgtcagcgga gctgtctact
     6061 gacagaagat atgggatatc ctctccccat gcgtttttgg cggattgggt atgaccgttt
     6121 gaagcagcgt gaaaggcgac gattatcggt tcgccttcat aggtgaggat atcgtccttg
     6181 acggcattaa cggcggattt aactttttgg taggcttttt catagtcgtt gccgtaatac
     6241 tctttggcgg ctttgagggt gaaaaagctt tggtagaggg tgctgtcatc gctgatgagt
     6301 gcgcctttta aggcgggagt aggcgactgg gcttcggaga gctgtcggcg gcatatgtag
     6361 gtatgggcga ggacggcttg cgctttcagg gcttcttcgt caaagtccgc aggcatttgt
     6421 gcaagcactg agcctatcat atattcttca agggtgaagt cttcggtctt gccgctttcg
     6481 gtgaaatata cggctatttt ttctttttct gaaaatgcag gtttcgatgc ggactcgttt
     6541 gggcgaagaa atactatggt cggtattatt ataaggataa cagcaaatac tgccattatc
     6601 tgtatcaggt ctttcatatg gtttgtcctt tcttttttgg ggtactatat tatattcatg
     6661 cagtggagca aatattcata cagggcaaat ttttgtactg caaattttta aatggcggag
     6721 atatgcggtt ttaatttgac atgttatctt aatacttgac aaaaatctga aaaaggtgta
     6781 taatataact tatatttata atatacttga aagcgagatg atctattcaa tgataaataa
     6841 tgcagcaagg tcgcttttat gcgaacagtt tatagcaaac aatactattg accctaagct
     6901 ttatgacaga tatgtggtaa aaagaggctt gagaaactct gacggtacag gtgttatggc
     6961 tggtcttact aatatctgta acgtacacgg atatgtggta aacgagggtg agaaatctcc
     7021 tattcaaggt cagcttatct tccgtggata caacataaat gatctggtgt ccaacgctca
     7081 gaaggagaac cgtttcggct atgaggagat agtgtatctg ctgctcatgg gcgatctgcc
     7141 taacagggag gagcttactg cgttcaaggg aatgatggct gaaaacagac ctctccctga
     7201 caacttcttt gaggacatga tacttaaagc tccgtcaaag aacatcatga ataagatggc
     7261 gagagcaatt ctggctcttt actcttatga cgataaccct gagaatcgtt ctcctgagta
     7321 tgaaatggct acggctatct ctataatctc taaattgcct aacatcatgg tttcggctta
     7381 tcaggtaaag aaacgctgtt atgacggtga aagcttgttc atgcaccctc tgatacctac
     7441 acattctact gcggagatga tactgtcggc actgcgtctg gacagacagt tcactgagga
     7501 agaggctaag atacttgatc tgttacttat gctccacgct gagcatggcg gaggaaataa
     7561 ctcgactttt gcctgccgtg tgcttacatc ttccggaacg gatccttatt ctgcatactc
     7621 tgcggctatc ggttcactga aaggtccacg tcacggcggt gctaacctga aagtggcggc
     7681 aatgcaccag tgcataaagg acaacgttca gaactgggag gacgagggcg agatcgctga
     7741 ttttctcaca aagatactca acaaggaagc ttttgaccac acaggtcttg tttatggcat
     7801 gggtcacgct gtttacacat tgtctgaccc tagagctgta atactcagag agaatgccaa
     7861 gaaaatggct gagaacactg agtttgagag agaatacaag cttcttgaag cggtggaaag
     7921 gcttactcct gagctgttca agaagatcaa gggcagtgac aaggcgatgt gcgctaacgt
     7981 tgatatgtac tcgggctttg tttacagtat gctgggtatt cctgaggatc tttacacgcc
     8041 tatgttcgct gtttcaagaa tggcaggctg gtgcgcacac agatttgaag agcttgtaac
     8101 aggcaagagg ataatccgtc ctgcatacaa ggctatctca ggtgagaaga agtatatccc
     8161 attggacgaa agataagcaa aataattttt acctgttcct aatgggcgga aaaaggcttg
     8221 aaatattttt gcgtatatga tataatagaa tcgggtatat gcccggttct atttttttgt
     8281 ttggtgtatc aggagggatc gcttgaaaaa tcttaaattg gctgctgttt gtctttcttg
     8341 tcttactatg acaggctgtt cggcactgaa tttttctgtt gagggactta taaacgcccc
     8401 gaagcttacc tctgagcaaa gcgagataca tcaggcgctt atcgagagcg tgggcagcaa
     8461 tataactctg aaatatccta aaaacggtga ataccgctcg gcttatgtta tcgcaaatat
     8521 cgacgatgag cctacggacg aggctatggt cttctatgag tacacttcaa acggatctga
     8581 ggaggacggc ttgagggtga atattctcga caaggacgag gacggcaagt ggtattctgt
     8641 gaaagagctt gcaggcgcag gcacggacat agatcaggtc attatctcgc ctatgggaag
     8701 caataaccag acggacgttt ttgtgggata tcaaaatctt actaatgatg aaaagactat
     8761 ggagatatac agctataccg acggagattt caagcgtgtg gcgcttgata cttattcggt
     8821 gctggagcct ttggatataa acaatgatgg ttatatagag cttatcacca ttcagagaag
     8881 caccaataat gatacaggtg cggtgacggc aaaggcttct atgctcaaca tgaaaaacaa
     8941 tgagataacc cgtggcgaga acgttgatat gtgcgataat gtgacttctt atgtgtcatc
     9001 aaagacggga atgcttgaca gcggcaggcg tgctattttt attgacggac tgactgctga
     9061 cggaaaattg cagacggaga taatatacta cagatattcg ggacttcaaa atcctatgca
     9121 gcttagaagc gaaaagctgc tgcctttatg cacaagacct gcgggctact acagtgagga
     9181 catggacggc gacggtgatg tggagatacc gtcaacttcg cctatggtgg gttatgaaaa
     9241 cgctgtggca gacgaaatgc tctatatgac tacatggagc gtttacgagg atttttatga
     9301 tctgaaaaca aaatacacag gctattatgc catatctgac ggatattttg taacgttccc
     9361 aaaacgctgg aacagttctg ttacggtgaa aaaggacagc gacacaaacg agcttgtttt
     9421 ctataaatac acgggagata taaacgagag caacgaggag ataatgcgta tcgctgtgtc
     9481 ttcaaagaat gattcggagg attatatctc tgacggatat gagcttatcg acagcaaggg
     9541 tcagcttgat tattttgtga agcttcctct tgacaggcgt gaaaagctca taccgactat
     9601 tgacgaaatt cagaataatt tttacataat aaactgatct tggagaggag taaatgtaat
     9661 gaaaagagtt ttggtttgcg aggacgaaga ttcaattaga gaatttgtgg tcataaacct
     9721 tgtaagaagc ggttacgagg tagttgacgt aaactgcggt gaggacgctc tcaaagccta
     9781 tgacgaaaag gacggcggtt ttgacgttgc tctgcttgat attatgatgc ccggcattga
     9841 cggaatgcag gtgtgcaaga agataagaga gaaaagcaac actatcggca ttatcatgct
     9901 ttctgcaaaa tcacaggaaa tggataagat atcatgcctt atgagcggtg ctgacgatta
     9961 tatcacaaag ccgttctcta tttctgagct tattgcaaga gttgacgctg tttgcaggcg
    10021 tgtaaatcgt tcaggctcta agcctgagga ggcttcacag atagtttcag gtccatttac
    10081 gcttaatctt aagagcagaa ctgttttcaa gaacggtgag cagatagacc ttacacaggt
    10141 ggagtatcag ataatggagt atttcttcaa aaatcagaat actgcacttg accgttcaag
    10201 tatactcaat cacatatggg gagagagcta ttacggcgac gataagatag tggacgtgaa
    10261 tatcagaaga ataagaatga agatagagga cgagccttct aatcctaagt atatccttac
    10321 tatatggggc tttggctaca agtgggcagg cggctctaac tgagctgtga aaacatgatg
    10381 aacggagttt ttatcagttg agcgggaaaa aaagaaaaag cggcagactc agtatcacac
    10441 agcactggct gaggaacaat ctcggggtgg tcgtgggaat acttgtggtg cttgaaatac
    10501 tgcttattgt ggtggtgagg aattattact acagctccac aaggcagtat cttacatcta
    10561 aaatgaagat agtcaccacg gcggtagtga acgcttcagg tgacgagcag actaactata
    10621 attctcagat acgctccctt gtggaaagct atgacgagaa agacaagata gagcttatgg
    10681 ctatcaagac cgacggtgag gtcgatgtta catcatcagg cttctctccc tcggagaatg
    10741 acaacatgac cgattatgag gacgcaaaga agtccgacac tggaataggc acacagatat
    10801 atgagctgtc aacaggggaa aaggtcatgg cgataacgtc agttatatct ccgaagggaa
    10861 gcgaatatga ggcactgaga atgttgtctt ccatgaaaaa tatcgaccat cagatcttta
    10921 ttatgtctat gataatcacg gctatcgttg tggctgtcat aatgctcatg tttttctcgg
    10981 gaatgttttt tatcaaatct attgttttgc ctataagaag cataaacagc ataacaaggc
    11041 ggtatgctac gggagatttc agcgaacgtc ttgaaaagaa acgaaatgac gagcttggcg
    11101 agctttgcga ctctatcaac tacatggcgc aggagctttc taatacagaa cagatgaaga
    11161 acgagttcat atcctctgta tctcatgagc ttagaactcc gcttaccgct ataaggggct
    11221 ggacggaaac tgttaccagt atgtatgagg acaaggaaac cttcaagaag ggtatgaggg
    11281 tcattacaag tgagacggag aggctttcgc aaatggttga ggagcttctt gacttttcga
    11341 ggatacagga caacagactc acgcttatta tggatactat agatattctt gcagagcttg
    11401 gggaaactgt gcttatctat caggagaggg ccaaggcgct tggtataaca ctcaattact
    11461 acgagccgaa aatgctgccg tttgtttacg gcgacaagaa caggcttcgt caggtattca
    11521 taaa
//
LOCUS       DAWEMR010000077        11508 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2372_length_11508_cov_12.459618,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000077 DAWEMR010000000
VERSION     DAWEMR010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2372_length_11508_cov_12.459618"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3286 a         2471 c         2581 g         3170 t
ORIGIN      
        1 gcaaaaatgt caaatcaacg ggcgaacact gttcgcccct acatacaaac agcgtgaata
       61 tgcaaaagca tatgagcata gttacaatat tatttattcc cttttctctg aacaatgaag
      121 atacctgagc atacaaggat aagtgcggct atcccctgaa cgccaaaaat gcctttttca
      181 ccaagcagga tagttgaaag caacactccg cacacagggt tcataaagcc gaaaatcgcc
      241 acctttgaaa cagggttgtg cttcaaaagc actgcccaca gcgaatatgc cgccgctgat
      301 acaaaggcaa gatacaaaag catgagaaaa gcctttgcac tcatgtgacc gagtctgccg
      361 cccattaaaa agcctatgac agtgagaact gctccaccga aaacgaactg ccagccgcta
      421 agcataacag ggtcttctgt ttcggaatag cgcttcatat acaccgatga aaaagcgtat
      481 gcacaggtgc aaagaaaaat aaatccgtcc ccgagaaaat tgaagcttac ccccgaaagt
      541 cccccaagat ttataatgat tatccccgaa aaaccaagta tacagcctgc tattttcaca
      601 gcggtgagct tttcaagctt gtataaaagc gatgacacaa gtatcgcaat aaaaacgttc
      661 gccccaacta ttatcgctgc ctttactccc gttgtgtggg ctagacctat gtagtagaaa
      721 aaatactgca aaattgtctg aaaaaggctt attatcatga tctttggtat ggaagtcttt
      781 gttggcagga gaatttttct ctccgctccg cttcccatgg caagagccat tagccctgcc
      841 acaaaaaatc ttatgccccc aaacacaagc tgcgaaccgc tgtcattggt ggttatgttc
      901 ataaggctgt agcctatttt cacacaggaa aatgcacttc cccacagcat acagcatatg
      961 gacgcaagaa cagccactac ccatgttttt gtcagaatat cttttttctc gttcatacat
     1021 tatcctatct atagcaaatt gtggatcttc acagaaagac cgcagcggcg taaaatgctg
     1081 ctgcggtcga aagttacttt ttgtcttttt ttgctttctt ttcctttttg cgttttttct
     1141 tgttttcctt tattatcttc aaagggtcgc cgaaacgttc cttatcgtcc ttgacaggtt
     1201 cgatagtttc gctctttgtc atgatatccg tcatttgctg taaaagcatc gactcctcga
     1261 acatatccac agcttcctca caggtgtcag ccttgccgtc cacaagaaac tgttttatct
     1321 gctgaacgtt cttcatatag tcaagtgaca tgaaaggcac tctcttttca agagccttgt
     1381 agtaatcatc tctctgacgt tctacctgct ttattctgtc atcaagcatt attatctgtt
     1441 cgtttatgcc ctcgttcact tcaacctgct tctgttcagc agcagtaagt ctgtatttgt
     1501 tcagtgcgat accgaaaacg cctgcaccga taaaaataag tgcacctgca ataatgccca
     1561 ctataataag ctttgtgcca aggagcagcc acagcagcag aaaagccacc aatccaatgc
     1621 cgatgcttat gcctaaagac ttaattattt tattttttat attgaagttt ttaacaacat
     1681 aatcgttgtc atggatataa gttttgaggt attctttatt ttccttgctg accgttacag
     1741 cgtcctcaaa ctcaaaatat ctttcaaagt attcggcagc acggtcaatt ttttcaagct
     1801 cagtttcgtt agccatatat actccctcag atcacgaaaa aatttctaaa agcagaacct
     1861 tttagtcttt tgcgtggtgc gtgatagtga acactgcaca aataacacgc tatatttccc
     1921 accatattac tacaattata acacacagca tgttttttgt caaccatatt aagcatagtt
     1981 ttcgtaaaaa taagacacgg acttctgtaa ttagattttt atagacaggt aaaaaaagag
     2041 gacgaacaca gtccgtcctc cgatatgctt aagtatctta ctctgctgtt ttgttgtttg
     2101 taacagcaat gtcaggagcc tcgccatttt cagcagcttc tttagccgcc ttgcgctcct
     2161 ttgctacctt tataagggtc tgcttgatag actccagcat ctcctgtgtg aaaggaaggt
     2221 tctcgccaaa tgggtttaca acaaagccct gtacctttgg cgtctgctca gtgagagcag
     2281 ctatatcctg aaatctcatt gcaaagctct ggaaaggctt gtcatactgg agcttcttag
     2341 cttcctctgg ggaagtgaaa acagggaaaa atgtgccgtt tgttttgttg ttgttgagaa
     2401 gaatgaactt agccttgctc ttgtcctcga aattaacatg gttttcagcg tcggcaacaa
     2461 gctctgttgt cttttctacc attgcaggca cgagaaatgt gcaggcaagt gctgtgttta
     2521 ttaccttgtt ttgagtttcg gcacagaaat tctttctcat ttctgcgata gcttctataa
     2581 gcttagggtt gttgtcgaaa tttggtttct gattgttagc cttgagttct gccattttta
     2641 attactcctt gtctgtttct tcaagatttg tttcgattat aaatttaggt ctagcctttg
     2701 tttccaaata gatcttgcct atatattctc cgataatacc tatagccaaa agctgaagtc
     2761 cgcctatcgc ccatatagac gtgatagttg aagcccagcc ctgaacagtg tgcccaactg
     2821 cccatgttat aaggaaatat ataagcatta taagtgatac taaaaatact attataccga
     2881 tagtggtgat aaatcttata ggctttacag aaagtgaggt tattccctca actgcaaagg
     2941 aaagcatttt cttcagcgga tatttagatt cgcccgccac acgctcgttt cttacatact
     3001 taacgatgtc gctcttgaag ccgaccatag gcacaagacc tcttagaaac agatttactt
     3061 ccttaaactc tgccagagcg tctatagccc ttttgctcat aagtctgtaa tcagcgtgat
     3121 tatatgttat ctcaacgccc atatgctcca aaagcttgta atatgcctga gcagttccac
     3181 gcttgaaagc ggtatccttt tctctcgatg aacgaacgcc gtaaacaata tcacagccct
     3241 ctgccctctt atcaaggaag ccgtcaatag cgtttatatc gtcctgcaag tcagcgtcca
     3301 tggaaacaac gatatcagcg tagtttctcg ccgtcataag accagccagc agtgcgttct
     3361 gatgaccttt gtttcttgaa agcttaagac ctgtgaaaac agggcttgct tcgtggagct
     3421 tctgtattat ctcccacgtt ttgtcctttg agccgtcgtc aacgaacatg accttgctgt
     3481 tctgtgcgat gcgaccttct gctttcagct tatcgaactt ttttataagc gcttccgctg
     3541 tgataggcaa catttgttcc tcattgtagc atggtattac tacatacata gtttctgtca
     3601 ttttgttctc ttatcctttc tcatattcga tcccgaacag gcggcaagcg tttctatttg
     3661 taatgtcaag cacttcctgc ggatcaagtt tttttatctc agccacgacc tttgccactt
     3721 cttctatcat agggctttcg cacctctgtc ctctgtaagg agggggcgcc atgtaagggc
     3781 agtccgtttc aagaagaagc ctgtccattg ggacaacttc aagagcacgt ctggcttttt
     3841 tagcattttt aaaagcaagc gcacctgtaa acccgatgta cataccaagc ccaagcacta
     3901 tctctgcggt ctccgccgag cctgaaaagc agtgaactac acctgacggc gcatatttgt
     3961 tcagcacgtc aaggcagtcc tgagtggctt cacggcagtg aactataaca ggcagacttt
     4021 tcttcacagc atattccacc tgcttgtcaa aaagctttat ctgtctttct ttgtcatatc
     4081 cctcataatg atagtcaagc cctatctcac cgaccgcaac aagcttgtga gtagagcagg
     4141 ctttcaaata aagctcgtca agcacctgca tataattttc aggaagagta tccatacact
     4201 cagggtgaaa gcccacagag gtatagaaat tctcatattt tttcgcagtt tcgattccga
     4261 acaaagacga tctttcgtcc gttgcggcgt ggcagacctt gtctacagga gagtcagggt
     4321 cgcaaagaag tctgtcaagg acttcgtatc tgtcctcatc gaaagcgtga tcgtcatagt
     4381 gacaatgtga gtcaaatata ttttttatgg caaggacgtc ctttcgtaaa aatatacaaa
     4441 catattatat cacaactcac caattttgta aagcttttta ttgtaaaaac gggcataaaa
     4501 aaacgggcga acaaaccgtt cgcccgcaaa gatctttatt ttactagaaa gctatcttct
     4561 tttcgatata agctaccagc tcgtcgatct tgatacgctc ctgctccatt gtatctctgt
     4621 ctcttactgt tacacagccg tcctcaacag aatcaaagtc atatgtgatg cagaatggtg
     4681 taccgatctc gtcctgacgg cgatatctct taccgataga acctgcctcg tcatagtcaa
     4741 caaggaagtg ctttgaaagc tctgcataaa gctcgcctgc ctgctcgctg agcttcttgg
     4801 aaagcggaag aatgcaagcc ttgaaaggag caagtgcagg gtggaaacgc ataacagttc
     4861 taacgtcagg cttctcctct gtgccgatgt tctcttcgtc atatgcttcg caaacaactg
     4921 tcaggaacaa tctctcaacg ccaagggaag gctcgataac gtatggaaca tatctctcgt
     4981 ttgtttcagg gtcaaagtaa tcaaggctct taccgcttgt cttgatgtgc tgattaaggt
     5041 cataatcagt tctgtcggca acgccccaaa gctcgcccca gccaaatggg aagagatact
     5101 caaagtcagt tgtagccttt gaatagaagc aaagctcctc aggtgaatga tctctcagac
     5161 gaaggttttc ttccttgatg ttaaggctaa ggagccaatc cttgcagaag cctctccagt
     5221 agttgaacca ctcaaggtct gtgccaggct tgcagaagaa ctcaagctcc atctgctcaa
     5281 actcacgaac acggaaaata aagttacccg gagtgatctc gtttctgaat gacttaccta
     5341 cctgtgcaac accgaaaggt actttctttc ttgttgttct ctggatattt gcaaagttta
     5401 caaagatacc ctgtgcagtt tcaggacgca gataaagctc agactttgag tcctcagtta
     5461 caccctgaaa tgtcttgaac ataaggttga actgacggat atcagtgaag ttgtggcttc
     5521 cgcaatctgg gcatgggatc tgcttttcct tgatgtagtc catcatctgc tcgtttgtcc
     5581 agcctgcaac gttagtgccg tcaaaatctt cgataaggtt gtccgctctg tgacgtgtct
     5641 tacagtcctt gcagtccata agagggtcag agaaaccgcc gatgtgacct gaagccaccc
     5701 aagtctgcgg attcataagg atagcggagt caagaccaac gttgtacttg ttctcctgaa
     5761 tgaacttctt tctccaagcg ttcttgatgt tagttttcag ctcaacgcca agcggaccgt
     5821 agtcccaagt gtttgcaaga ccgccgtaga tctcgcttcc ggggtataca aagcctctgt
     5881 ttttacagag agccacgatc ttatccatgg ttttttcaga attattcatg aaacattctc
     5941 cttatacaaa atataatact accattatat tttatagcaa aattccgcaa atgtcaaggc
     6001 gtttcagcga tttattactt tgtgtagtta tatggctttt tatcgttgca aaaaatcacg
     6061 cattgtgcta taataaaata gtatcgctat gactattcat gtattggaac atattaggag
     6121 aatttgactt gatgaacagt ttttttatcg gaaacgttga aatagaaaag accgccgcat
     6181 tagcacctat ggcgtcggtg gcagataaat catatcgcct tatgtgcaaa gagtatggtg
     6241 cgtcatatct tgtgagcgaa atgatatcct cgaagggtct gtgctttggc gacaaaaaga
     6301 ctgcacgtct ttgcgagatt gagaaagagg aacgccccta tgctttgcag ctttttgggg
     6361 aagaccctta ttatatggga aaagcggctt ataagctcaa cgaatattcc cctgatatca
     6421 ttgatataaa catgggctgt cctgtgccta agatagtagg caacggcagt ggctctgcac
     6481 ttatgaagct tcctgacact gcggcagaga tatgcagaga ggtggtaaaa aacgcaaact
     6541 gccctgttac agtgaaattt cgtgcaggct gggacgaaaa cagcataaat gcagtggagt
     6601 ttgcaaaact tatcgagcag gcaggggcaa gcgccgtcac ctgtcacggc aggacgagaa
     6661 cgcagtttta ttcaggcaaa gccgattggg atataataaa gcaggtaaaa gccaacgtta
     6721 agatacccgt tataggcaac ggcgacgttg ttgacggaga aagcgcaaag gcaatgtatg
     6781 agcagactgt ctgtgacctt gtgatgatag gaagaggaag ctacggcaga ccatgggttt
     6841 tccgtgacgt gaagcattat cttgaaacag gggaaaagct tcctgaggtg agcatagaag
     6901 aaaaaatggc agtgatgaaa aagcactgcg agcttatatg caaatacaag ggcgaacgtc
     6961 agggcatgaa agaagccaga aaaaactgcg catggtatgt taaagggctg aaaggctctg
     7021 caaggctaag agcgcagtgc ggtgggctta ataccatttc tgacctttat gagatgatag
     7081 accacatact tgcaaacgag ttaggagaat aaacaatggg aattttatcc gtagtaggaa
     7141 cgcctatagg caaccttgga gatatgacat accgtgcagt tgagacccta gaaaaggctg
     7201 attttatctg tgccgaggac acaagagtta ccgcaaagct tctcaattat tttgacataa
     7261 aaacgccact tgtgagctat cacgaacaca atgcaaaaca ggtgggcgag agcattttga
     7321 acaggataat ggcaggcgag aacgccgcta tcgtcacaga cgctggtatg ccctgcattt
     7381 cagaccctgg ggagcttctt gtgaagctgt gcgctgaaaa cggtgtgaaa gtggaggtag
     7441 taccaggtcc gtcagcggtg gtatcagcac ttgccataag cggacttgac actgcaagat
     7501 ttcagttcga gggctttttg tcaaccacca agaagcaaag attaaatcat cttgcctcgg
     7561 taaaaaactg cacgaacact cttattttct atgaagcgcc ccacaagctc atctatactt
     7621 tgaaggatat gctggaatat ttcggtgaca gaagaatatc tctttgcagg gagcttacaa
     7681 agatacacga agaagttttc cgcaccaccc ttgcgcaggc tgtggagtat tatgaagcca
     7741 acaagccaaa gggcgaattt gtgcttgtta tcgagggtgc aaaagaggac gagctttgcc
     7801 ctgcggagaa tatagaggaa gcgcttgaac aagtaaaagc ccttgtggaa aagggtatgc
     7861 gtggagctga cgcttgccgt gagatagcaa aggcgacggg attttctaag ggggagcttt
     7921 acggactctt gtttaaatga ggaatgagga gtgaggaatg aggaattaag gtatcgcctg
     7981 cggcgatgtt tatttcttgt tttttcgcat ttgtattata tataaattta gattttttga
     8041 ggtgatttta tgagcataaa agaatacata ggcggacagt gtgctaatcc agagggactg
     8101 ggcggattag cctgtgcaaa attcatgaat tttttcaatg cagagcacta cagagcggtg
     8161 aaaaaatttg tctgcaagct tgacggtgca aggatattgg atataggctt tggaaacggt
     8221 gtgactatca aaaagcttag caagaatata aacgctaagt tctatggcgt cgatatttct
     8281 gcggatatgg tggaaaaagc caagcgagaa aaccgtgacg gagtgaatac caacaaggtg
     8341 atcttacagc agggaaaagc tgaggagctt ccttataatg acgatttttt tgacactgtt
     8401 tacactgtga acaccattta tttctgggag gacactgcaa aagttcttga cgaggtaagg
     8461 cgagttctca aagacggcgg caattttatc tgcacattct atacaaaggg ctaccttgat
     8521 aaactcccgt actgcgacac aggttttgac aagttcactc ctcagcaggt gcgttcaatg
     8581 gctagagagc gtggcttcca tgatgtaaaa gtcaaaattc tgagcgactg caaagcgtat
     8641 tgccttgttg gcacgaaaat agagggagag taaggtggca gatgtgacca ttgtgcctct
     8701 ttcagaggaa aatgtacagc ttttttcaca aactgcggcg aaaattttgc ccgaagcttg
     8761 gagttttgaa acctacaaaa aacagctttc aaaccctgac gacatatcgt ttcttgccct
     8821 ttgtaatggc gaagccgcag gctttataag tgtgtggtgc gtctgcggag aagctgagat
     8881 aaacaatata ggcgttttgg agaagttccg cagaatgggc attgcaaaag ccctttttga
     8941 cagcgcatac aatgccgcaa aggctgaaaa atggtatctt gaagtgcggg aatcaaacct
     9001 tgtggctata agcttttacg aaaaattggg ctttgagagg gtaggcatga gaaaaaattt
     9061 ctacaccgcc cccacggaaa atgctgtttt aatggcactg caacccactg aaaatggaga
     9121 aataaatgac atataacgat tttacatttg agtccttggg cgagggcatt gagatatgta
     9181 tatctgacga acaccgtttt ggtactgacg cttttttgct tgcggatttt tcagcaggac
     9241 ggcacaagga tatctgcgca gacctttgca caggaagcgg cataatagcg cttcttctgc
     9301 acaaatttta caagccacag aagatatatg ctgttgagat acaggaaaag gctcacgaac
     9361 agcttaaagt atctcttgag aggtcgaaaa tagatactat ccagcctgtt cttggcgacc
     9421 ttaacgactg gcgtagcgaa aaggaacttg atcttatcac ctgcaatccg ccctacaaga
     9481 taaacaacac aggtgcaaaa aatgacagcg aagcggtttc tatcgcacgg catgaaatgc
     9541 tctgcactat tgatgacgtg tgcagatgtg caaagaaaaa tctgaaattc ggtggcaggc
     9601 tttgcatatg caaccgccct gagcggcttt gcgatataat gacggcaatg agagcgaacg
     9661 gtatcgagcc aaagcgtttg cggacagttc acaaaaaccc agagtgtccc ccatggctta
     9721 ttcttgtaga gggcaaaaag ggcggaaagc cttttgtaca gatagaaaag cctttgtatg
     9781 tgcgttccgc agacggcgga aactcggcgg aaatgaagag aatatacgga cttccttgaa
     9841 aagtgaggaa tgaggagtga ggaatgagga attaaggtat cgcctgcggc gatgattatt
     9901 ttttcgtagg ggcgaacact gttcgcccgc ttgctccaat ttgtctgcgt gtgagttgaa
     9961 atcaaattat ggattatggt gttatgacgg attatcgagt cacgttccac ataagcgtgt
    10021 gagttgaaat gaagtgacca attttctgtg ggtaaatcta aggacatgat atgcgtatat
    10081 ttacgaaata aaaagagggt aataaaatga agattttagg aatagaaagc tcctgcgatg
    10141 aaacagcttg ctctgttgtg gaggacggaa aaacggtgct ttcaaatata gttgcgagtc
    10201 agatagatga acacaggctt tatggtggag ttgtgcctga gatagcctca cgccgccatg
    10261 cggagaacat aatatgggtg acaaaggccg ctcttgaaga agcaaactgc acaatggagg
    10321 atatcgacgc tgttgcagtg acctacgccc ctggtctgat cggagcactt ctcgtgggag
    10381 tgagctttgc aaagggactt gcaatgagtg caggcaagcc tcttgtgcct gttcaccata
    10441 tcgcaggtca cattgccgca aactatatct cccaccctga gcttaaacca ccttatcttt
    10501 gcttggtcgt gtcaggagga cattcacata ttgtagaggt gcttgattat tcaagatatc
    10561 atgttatagg cagaacaaga gatgatgccg caggcgagtg ttttgacaag gtagcaagaa
    10621 cattgggctt tgaataccca ggcggaaagt ttatcgacaa tgccgcaaag tcaggtgatt
    10681 ctaatgccta taagctccct cacccaaagg tacagggcag cgagtatgat ttctctttca
    10741 gcggactaaa aactgccgtt ataaatcttg tgcataatgc cgcacaaaag ggcgaaactg
    10801 tcaatgtgaa cgatatggcg gcttcgttcc agaacaccat atctgacatt cttgtagaca
    10861 aagcaatgct tgccgcaaaa gatcttgggt atactaccat aggacttgca ggcggcgttt
    10921 cggctaactc aggggtaaga gaaaaattgc agaaagcctg tgacaagcgt ggctataagc
    10981 tttatatgcc tgagttcaaa tactgcggag ataacggtgc aatgatagcc tgtcagggat
    11041 attttgacta tcttgcaggc agacgagcag acgaaagcct taacggcatt gcaacgctgt
    11101 cgctggataa gatttcggaa taatcttatg gataagcctg taaggaaaat cataagactt
    11161 gacaattatg attattcaaa aaatggactt tattttatta ccatatgtac tgtccaaaaa
    11221 aacgagttgt tgtgggcaac agatgtaggg gacggcgccc ccgacgtccc gttatgttgc
    11281 gcacacgctg tcggaaaggt ctatctttca agctttggta aaattatcag agataatatc
    11341 cacagagcaa acagtgtcta tgatgacaaa aagatattgc aatatgtcat aatgccaaat
    11401 catatccatt gtattctgaa attcggtgcg ggagaaagcg ggacgtcgag ggcgccgtcc
    11461 cctacaaatg aggtagttcc acagtatgtg tcaatgttgt aaggattt
//
LOCUS       DAWEMR010000078        11170 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2468_length_11170_cov_9.828880, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000078 DAWEMR010000000
VERSION     DAWEMR010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11170)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2468_length_11170_cov_9.828880"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1425..1434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1772..1781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2916 a         2679 c         2101 g         3454 t
ORIGIN      
        1 atccgatgca gactcgccct tgccatagcc gtaaagtgca atatcagaat atgaaagctt
       61 aacagcgttt atgcccacct cgttgttgga aaagttatca agggcataca gatttttact
      121 gctagatttc tcaatatccg caggtgctgt cattgtatat cccgtaccct gtaatgtttt
      181 cgtagctgtg tttttgtaaa aatatctcac accaaaagcc accaagaaca caaggataaa
      241 cacaagaact gacatcaaag gctttggtat acccttttta gcagatgtaa caggcgctat
      301 cttataaggc tcatatgcac tgccgtatgg agtttgagca ttgccattct gcggaggcgg
      361 ataactctgc tgagcgtagc cgttctgtgg ctgaccataa gactgctgac cctgagcata
      421 attctgctga ggtactgcat tcacaggctg tgcacccgta gtgttgcgct tgccaaagcc
      481 aacattctca ccaaggtttg atgtgtcgat agaatccata taaccgcttg tttcagactt
      541 cacagccgac ccacaattag gacaaaaagc gtcagtatca ctgatctttg ccgaacaaat
      601 aggacatttc atattctttt ctccctcata taatttatgc ttttattcta acatatgctg
      661 tttattatgt caataattta acaaatcgat tttataaatt ttgtattaat tgatatggat
      721 agtgcttgcc cacttttcaa atttcgggtc atagaaatcc ttttcatcag ccttacagcc
      781 aaaaacaaac caataatatt tataacctga ctttttgcat atagcatagt tataaacttc
      841 gtcgcccgtt tttgcacgat aggtcatttt aagcttgtta ccatcagcat acacttcttt
      901 atagctgctt ccaaactgac ttttcaaatt gcccttcatg taggtgacaa acagtttctc
      961 ggtaagaata tccgttgttt cgccggcaag tgaagagtaa tcaaggatca tatactgaaa
     1021 atcgctatta acatcggcgt actttccgtt ggcttttaca gttccatcac tgctctgtgc
     1081 cgcataatct attgtggttt ccttttcaag tgaccttggc agttcaacgc taaagttctc
     1141 ctctctaatg gtatgggttg aaacattgtg atagtaccaa tatccaaaac cgatagctgc
     1201 gcccacaaac aatgccagca aaaggataag cacctttttg cctatagaca tattctttcc
     1261 cactgctctc acattgctgt taagaccatt gttaaagcta ttattgaaac tattgccata
     1321 cctcggctgt gccgaattta cccctgtcat ttcaataact cttgtgccac aattctgaca
     1381 gcaaatgctg ttaggcagaa tatcgcaccc gcatactgta catcnnnnnn nnnncttctt
     1441 agcttcgata tcagcaagag cgtcttttct tgaaagattg atcctgccct gatcgtctat
     1501 ttccattacc ttaacaacga tctcgtcgcc aacagaaaca acgtcctcaa ccttttctac
     1561 tcttgatttg tcaagcttag aaatgtgtac caaaccgtcc ttaccaggag ctatctcaac
     1621 gaaagcaccg aagttcatga tctttactac cttacctctg tagatagcac ctatctcagg
     1681 gtcattagcg atagtattga tgatattgat tgcctgttca gccttttcct tgtcgataga
     1741 tacaacagag ggttcctttg taggatcgta gnnnnnnnnn nacgtcacac tcagcagaga
     1801 tcttctgaat tacctttccg cctgaaccga taacttctct gatcttgtca accggtacct
     1861 ttgttgccag catcttaggt gcccacttgc ttacctcagc acgaggagca gggatagcct
     1921 tgagcattat ctcatcaaga atatacattc ttgcttttct tgtcttttca aaagcacttg
     1981 cgattatctc aggtgtcaga ccgtcaacct tgatatccac ctgaatagct gtgataccgt
     2041 tctttgtacc acctacctta aagtccatat cgccgtagaa gtcctcaagg ccctgaatgt
     2101 ctaccatagt catccagctg ccgtcctcct tagtgataag accgcatgaa atacctgcaa
     2161 caggctcttt gataggaacg cctgcgtcca taagtgcaag tgtagaacca cagatagaac
     2221 cctgtgatgt agaaccgttt gaagaaagca cctcagatac gcatctgata gcatatggga
     2281 actcttcaac agaagggata accggaacga gtgctctctc agcaagtgcg ccgtgaccta
     2341 tctctcttct tcccgggcct cttgaaggtc ttgtttcacc aacagaatat gaagggaaat
     2401 tgtactgatg catatatctc ttggaagttt cctcatcaat gccatcaagt ttctgagcgt
     2461 ctgaaacagg gccaagtgtt gcgatagtca ttacctgtgt ctgacctctt gtgaagatac
     2521 ctgaaccgtg tactcttggg agaacgccaa cttcagcagc aagtggtctt atctcgtcga
     2581 tacctctgcc gtcaacacgc ttgccctcat agagccagtt tcttactatc ttcttctgga
     2641 gcttatacat aacttcgtca agaacagctg tgttatcctc gcactgctcg tcgaacttct
     2701 catgaacagc gtcgattatt ggctgaagtc ttgcgtctct tacattctta tcatctgtat
     2761 caagagcgac ctttacctgc tcgcctacca ttgcttcaac agcatcgaac agatcgtggt
     2821 caacttccat tgactcaaac tcaaacttag gtttaccgat ctgagccttt atatcgttga
     2881 tgaaagctac cattttcttt atctcttcat gaccggtcat gatagccttg agcataagat
     2941 catcaggtat ctggtcagca cctgcctcga tcattactat cttttcagct gaacctgcaa
     3001 ctgtaaggtt aagtctgttc ttagctctct gctcaagagt tgggttaaga acgagttcgc
     3061 cgtcaacgta acctacgttg atagatgcaa ctggaccgtt ccaagggata tcagagatag
     3121 aaagtgcaat agatgtagct atcatacctg tgatctcagg tgagttgtca gggtcaacag
     3181 aaagaactgt cataacaact gaaacgtcat ttctcatatc ctttgggaaa agaggtctga
     3241 taggtctgtc tacacatctt gaagcaagga tagccttgtc agaagccttg ccttctctct
     3301 ttgtgaaaga acccggtatt ctgcctactg catacatttt ctcctcaaag tcaactgaaa
     3361 gtgggaaaaa gtcaacaccc tctcttggct ttgcagaagc tgtaacgtta gccattacaa
     3421 ccgtttcacc gtatcttacc cagcaagagc cgttagcaag tccgcaggtc ttacctgttt
     3481 cgactactac aggtctgcct gcatatgtcg tttcaaaagt tctgaaattc tcaaacattg
     3541 tctttcctcc attttagaaa tttagaattc tctgtatggt gaaagcatta gcaataaaca
     3601 ggacatacga gcttcataca ttctgtttaa tgctaagtta cctttcacct acagttcaaa
     3661 taaacaccaa aaggcagtgg caataaatgc ctctgccttg atgtcagtta catgattact
     3721 tacggatacc cagcttttcg atagtagctc tatatctttc gatatccttc ttcttgaggt
     3781 agttgagaag atttcttctc ttaccgatca tcttgaacag accacttctt gagtggttgt
     3841 ccttcttgtg tgtcttaaga tgttctgtaa ggtcgctgat tctctttgta aggatagcga
     3901 tctgtacctc aggtgaacct gtgtctgttt cgtgagttct gttagcttcg ataacagctg
     3961 tcttttcgtc ttttctaagc atttttttgt acctctttcg ttaaaatatt tccgatgtaa
     4021 aagaatatgg caggtaactt tcccacttac gggcgtgacc catattctgt tgcatggata
     4081 tgttgcagta agcaactcat atattataca ttatttcaga caaattgtaa atagtcttaa
     4141 cagaaatttt acaactattc tatgccatga tgagccttgc ctgctgtgag tgtgtttctc
     4201 ataagcattg caatggtcat agggccaact ccgcctggaa caggtgtgat aaatgaagcc
     4261 ttatccttgc agtcctcaaa atcaacgtca ccgcaaagct tgccgttttc atcacgatcc
     4321 ataccaacgt cgataactac tgcgccctcc ttgatataat cagcggttat catctttggt
     4381 ctgccaacag ccgcaaccag tatatctgct ctctggcaaa cttctttaag atcttttgtc
     4441 tttgaatggc agatagtaac agtgccgtta ttgtgcagca acagcatagc ctgaggctta
     4501 ccaacgatat tgcttctgcc gatcactaca cactccttgc ctgcaatgtc agtacctgtt
     4561 gacttgataa gctccataac gccggcaggt gtgcatggca gaaaactgta atcgcctatc
     4621 attattttgc ccacatttac agggtggaaa gcgtcaacgt ctttgtcagg tctgatgtta
     4681 tttattatca ctttatcgtc aaggtgcttt ggcagtggga gctgaacgag tattccgtcc
     4741 actctgtcgt catcattgag ctttttgaca aggttgagaa gctcttcttc cgttgtttcc
     4801 gcagggagag catacttgaa tgaattaaag cccacagttt cgcaagcctt ttccttgttt
     4861 cttacataga cctgtgaggc aggatcatca ccgacgatga ctacagcaag acctatctct
     4921 atgccctgct ctttaagctt ctctgtttca agtcttattt cttccttgac cttggctgat
     4981 actgccttgc cgtcaattat ctgtcccatt tattcttcct ccgtatcctt ttcatctgta
     5041 tcttttactt cgctgtcctc gtcgttatcg tcaccaagga tagacttata ctctgtctcg
     5101 gctttggtgt gtgactcctt gtccttttcc tcggcttctt taagcttgtc tttttgagcc
     5161 tgcttgccga atttttcgtc atattctttt tgaagctcaa taaagctctc acccttatcc
     5221 ttggcttcct ttatcttatg tttaagctct ttcttttcct gctgcatatc attatatctg
     5281 ttatactgct caagctgtgc ctttgaaagc tctgtatcaa cgaacagctt gtaatcgctg
     5341 tttctctttg tgatacctct gaacacgatt ataagtacaa gtgaagcaag aacacaagtg
     5401 cctgcaacaa gctgtgatac tcttatgttt ccaagataga gcgaatctgt tctcagaccc
     5461 tcgatgaaaa atcttcccag accgtaccag cctgtgtaca taaggaatac ttcgccgtca
     5521 aacttgcggt gtttaagata gaaatgcagg attataaatc caataagaca ccaaatagat
     5581 tcataaagga agcatgggtg gatagggtca agagccgata ctttgccgcc aagctcgtca
     5641 taatggtttg agatatattc cattgtggtg ttgcttatca tgccccaagg aagcttggtg
     5701 tttgcaccga aggcctcctg attgaagaag ttgccccaac gtccgataca ctgaccgata
     5761 agaaaacatg gtgcgaccac gtcaagaagc gcagtaagct tcagctttct tacttttgct
     5821 ataattccgc ccacgattat cgcaccgata agtccgccgt agatagcaag acctccgtca
     5881 cgaatatcga aaaactctga gaatttcatg ccctctgtat taaagatgat ataataaaat
     5941 cttgcgccaa agatagctcc aataagtccg ccgataacgg aatctgtcgc tctgtcaggg
     6001 tcgatgccta cggtcttcat tcttctgaag ccgtatatca tcgcaagaag aataccgatg
     6061 gttattaaaa aaccatacca atatatcttg aaattcgtac ctggcactgt aaaaagcact
     6121 ctgtcgatat caataccctc tttgaagata ttgtcaccct ttccgaaatt ggggaaataa
     6181 actctgttcg gaaactctct gagtttttcc cttattgcgt catttgccga agagtcggca
     6241 agaaactgca atgctgtcat aaatttatca ttcctttcac tgagtctgtg gctcaaaata
     6301 cctacttatt atctaccacg gagatagacg acttttcagg gtcgaacatt tctcttacgg
     6361 cgttttcgca atcctccaca gtcatttctg caagaacctg tgcttcatag aaagcgtcag
     6421 agttttcaaa atagctttct gcaagataat ctgctgctct ttcaggatta ttcattcctc
     6481 ttactatttc atatcaaact gcgacttgtc aaagccctct ttctttacac gctctatctc
     6541 agctagataa cggcgataaa gctcttcggg gtcatgggat tcacctgata ttattggaga
     6601 gaatactcca cagcttgtga acacttcgac accgaactgc gaatttatca gtccgtcatc
     6661 aaaaagctgt ttataaagag gtgacgaaga gccaatgagc agatgaagcg caactgacgc
     6721 tgttccagcc tttttaaaaa gttctctgcc ctcataaggc ttgcacttga agccgatatt
     6781 gaaaagtggc agacccacag ggaagctctg aaatatcttt ttctgcgcta cattctcagg
     6841 ctcatcaggg agctttgttt caagctccat attctcgcat ggcttcaaca ttttatcgca
     6901 tatttccatg accttatcct cgtcaacatt tcctgcaatg gcaaggacca tgttgtgaag
     6961 attatagaaa gtgtagtaac acttataaag aagatcaggg gttatctcag caatagtatc
     7021 aacagttcct gcaatatcta tctttatagg gtggtttaca tacagagctt tcagcagatt
     7081 ataaaaacac tgtctttcag gagaatctgc acacattttt atttcctgac cgattattcc
     7141 ctgctcctta gccacagatt gctctgtaaa atatggcttc tgaacaaagt caagaagtat
     7201 ttcaagagaa tcctcccagt tttccgagca gctgaacgta tatgccgttt catcaaaaga
     7261 cgtgaaagca tttgcgttgg caccagtttt tgcatataag tcaaaaacat ctgtgtcctc
     7321 gttttcaaaa agcttatgct caagaaaatg ggctatgccc tcaggcacag tgatgaaatc
     7381 tttgctgtcc ttggtcttga aacagttata aattgagccg tactttgtgg caaatatggc
     7441 ttctgtagtt gtgaagccct ccattttcca cacaagcacg tcaagtcctg acgggtgatg
     7501 aaaaacgctg tagctctcgc ccagtcgttc gctctttacg atttccttat tcatcacagt
     7561 cctcctcttc ctcgcccctg atcacgaaaa cagtatcagg cttaaaagtc tttgcacagc
     7621 ttattatgtc ctcacgggta agctttgcta tccgctcgtt tatctcctca ggggtgaaag
     7681 ctgtgtttcg tgtgttctgt gcagcatacc aacccatgat atcatatacg ctgtcataat
     7741 ttgacttaaa accaccgcat agagatttct ttgtgttttc aagctcctct tctgtgaagt
     7801 cgccattaca tatggcttcg agctgttctt cgatagcttt ttttgccttt tctatgttac
     7861 agctttcaac gccactatca ataaaaagag tgccctttct gtcggcatat gcggaagaac
     7921 aataatagca aaggctcatc ttttctctta cgtttgcaaa aagctttgaa aaaggcgttg
     7981 ctccgagaag catacagaaa agctttgcgg tgtagatatc ctcataatca gacttgaatg
     8041 ccatgaccat tttgctttgg cttacctgca ttttaagctc tctataaaca ggctctgcct
     8101 ttgcaggtga attgtgtctg tagtctattt tttccacagg cgaaactctt tcgagggcag
     8161 ccatttcatc tcttataagc ttttccgcag cagcagtgtt tccaccgcct gatatggtta
     8221 tctctatctt tgcactttca agaagctttt tgtgctgtct taacaaatcc tcctgagtta
     8281 tgttctccgc aagctccaca gttccaattg cagacacagc cgcaggctcg ccctcaaata
     8341 tgaccttttt tgcctgcaaa agtgcatagc cacgcttgtc attgacagtg gcaacaatag
     8401 cgtcgataag ttcctgcttt ctcaaagtga aatacttctc acagaacttt ccgtcaacaa
     8461 ggtgaggtct gagcagacag ttcaaaagct gtcttacaac ctgaacggat attacttcgc
     8521 cgtctatagt ataatcgtcg ccgataaagc ttgcagacag accgacagac tgataatcgc
     8581 ctatggtacc acagttggtg ccgatacttg agccgtaaag ccctgtgagt gtagaagtaa
     8641 gctttgttcg tgaaggtatc tccgcattcg atgtcacaag cactgtttga agcattgcat
     8701 ttacgcaggc tgttttttcg ttcagcggtg atataaatgc tatcttcacg cagtttgact
     8761 tgaatttctt gtcatatatt cttgtaagag atatccctct gccgagttct tccctactat
     8821 aagatacagc catttgatta ctccttcaag cgttttcacg ctaaaatttt tcgtgataac
     8881 acgataataa tatagtatat cacattcctg tgcagatttc catacttttc acaagaattt
     8941 cataaatgca cttattctat taccgtataa atgaaatttt cccagtgaag cattgttccc
     9001 tcgtctgttt cgtcaggcaa aagcgctctt gccataagaa gatcttcgcc tgtgtccaca
     9061 ttgagaagat gtgcataatc gccgtcaatt tttgttacct tatatgttct tggctccatt
     9121 tttattctcc ttttctgtcc ataaatacca cgttttcagc gcccagtctg ttttcaagtt
     9181 cgacaagcag ttcaggacac attttcacag catgaacaga ctttatgcct gtcattttgc
     9241 gtatatcgct gaaaaatacc gcaaactttg aaccagtctg tgctctgtat ttctcggcta
     9301 tagttctaac agcctttatg ccctctgcat ctcttgaaac aagccttacg caaatatccc
     9361 tttgaagctg taatgagcaa aatctatcga caggctcgag catatccgca agtatcttag
     9421 gttcttcttc ctctttacat gatatcttgc ctttgacgtg cagaagcgct ctgtctttta
     9481 taaggcctcc catagtttga aaaactgacg gaaaaaccac tatctccaat gttccgaact
     9541 tgtcttcaag ctccacaaaa cacatcatat cgcctttttt ggtctttagc atttttctgc
     9601 ccctgaccat tcctgcatag tctacttccg ccccatcagc gaaacgttta tcagccattc
     9661 cgccctcgtc tgtataggct tttatgagct gtgcagaggt gacacacctt gtggctgaca
     9721 tcacacactc aaagcggtct atagggtggg ctgatatata cataccaaga gcttcatgct
     9781 caaattcaag aagctgctgt tcgtcaaact cgccgcagcg ttctatcttc ttttccattg
     9841 gcaaagcggc acccatgcca aacaagtcaa gttgaccctc aatcacattt ccgtctgacc
     9901 gagttacgct tttcattatg tcctcacaag cattgagatg ttcacgtctg gtgtatccaa
     9961 aaccatcaaa agctccactc tttataagtg cttccactgt tctcaggtgg atatctctgc
    10021 ccgaaactcg tgtacaaaag tcttgaagcg aacggaacgc tccacctgat ttacgctcag
    10081 aaactatcct gtcgatagcc ccctcaccaa gcgacttaac cgcaagaagt gcaaagcgta
    10141 ttccctcagg ctctgccaca aaaccgcttt cgctcttatt tatatccagc ggcaaaattt
    10201 taacgccact tttgccaaca tctgcgatat atccgtaaag cttgtctgtg ctgtcaagaa
    10261 gcgtgatagt cataagtgcc gccatgtatt ctttatagaa atgacatttt aaatacgctg
    10321 tctgatatgc gatagttgca taagccgccg catgggattt gttaaaggca tagcttgcaa
    10381 aagaggtcat ttcgtcaaat atctcattgg caacattagc tggcacaccg tttgccactg
    10441 caccacagca ctgccctttc tcaccgtaga taaacgcttt tcgctcgttt tccaaaatag
    10501 ccgctttctt tttagccatt gctcgcctta cgatatccgc tctgccgtat gaatatcctg
    10561 cgagtgtgcg gaatatctgc attacctgct cctgataaac tatacagccg tatgtcactt
    10621 caagtatgcc tttgagcaat gggtgcttat acactatcct gtcaggttaa tggcggtttc
    10681 tgatatatgt tgggatcgag tccataggtc ctgggcggta aagagagata acagcgataa
    10741 ggtcctctaa tctttcagga cgaagcttca tgacagttgc agtcattcct gcactttcaa
    10801 actggaaaac gccctccgtt ttgccctgtg acagcatttc atataccttc ttgtcatcaa
    10861 gaggtatctt ctccaagtca aaatcagggt gagtttctat cacctgcttt cggcagtggt
    10921 ctataatggt aaggttgcgg agtccgagaa aatcaatttt aagaagtccc aatcgttcaa
    10981 gcactgtcat tgtatactgt gttgacacct gaccgtcacg ggcatagagc ggcacataaa
    11041 aatccacagg ctcttttgtg ataacaacgc ctgctgcgtg ggtggacacg tttcgtggca
    11101 taccctcgac gctcatggca atatcaagaa gttgtcgtat cttcatatca gtcttataaa
    11161 gggaactaag
//
LOCUS       DAWEMR010000079        11126 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2479_length_11126_cov_8.697769, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000079 DAWEMR010000000
VERSION     DAWEMR010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11126)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11126)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11126
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2479_length_11126_cov_8.697769"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2731..2740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3077 a         2364 c         1929 g         3746 t
ORIGIN      
        1 atgctatatc cttgatcttg ctcccactca actaaatact cattataatc gttaatggat
       61 ttgtctgaag caatttcgct tgctttgaaa tataattcac ccgatttatt tatcgcattt
      121 cttattctta gatatggtaa aagaatattg attttttctg ttccagatgc actaagtatt
      181 tttgttatac taacttcagc tgttttacta tgtggtaaat ttgctagaag cggattgcgt
      241 tcaaatggac gacatcctga tgcaaggtaa agaggggaac cagctagcat ttttgtgaat
      301 ctattaggca ttacatcaat tagacttttt tcacgaagat taatcaaaag ataacgaaca
      361 acattttttc caaatattac ggaatcggaa gaataatctc ttctcaatat ctcaatcact
      421 tctctaaagt gtttagtgtt tgtattacca tatatttgat cagtaattga atggaaatcc
      481 atttcttgta aatcaataag atcaagcaac gtcattcccg ttttggtgag aaaactcatt
      541 aattcagcat actcgccata gtttgcattc aaacttaatg gaatatttaa tattcttcca
      601 agtttgttta aacaagtagg tgcaatggat accctccagt ttgtgatgat ttttatattt
      661 gaagcaatac cccataaatc aatcgttgtg tcggtatagc caatattaat tgaataacca
      721 gttgaaatat tctgctttga ataaacagtt attctattaa atttagtagc atatttattg
      781 gctaattgca aggtgatttc ataataacgt ttggtgccaa cataaaatgg cattttcttt
      841 tgaacataaa aacgtgtgct ttccatagaa cgaggtgata aatccgtaga ttcaatagct
      901 tttgcaacta tattatagta ctcgttgtca agttgatccg tatttagtgg gaacttttca
      961 aggttttgca agattaaaat cttgtaaata ttatcgaaaa attctctaat ctgccacatg
     1021 aagttatagt attttagcat caatctttca gattgaccat cttccggaat acggcaatca
     1081 atatacttca atcctcttaa aaaattagca agatatttag ttctcaaatc gagttgagct
     1141 tgttcatatg ctaattgaag atttggcttt gagcatccaa gattataaga gtttagtaaa
     1201 agagccgctc tacaaagatc atataatttc ggtagaatat cttgtgagac ttcatctcta
     1261 gttgcgccaa ggctttctat tgtatcaata cttctgacta tcgacctatt acaagccatt
     1321 acaattcttt tgatctcatc aagattaagc tgagttgaac taagcttatt tacaaatgaa
     1381 tgatgttcaa ttatacgatc aagtttttta caaaaatcaa taatactttc atcagtatta
     1441 taaatttcct ctgagggggc gaaatcaacc gttatacttc cttgagtgaa atttatagag
     1501 ttgatatagt ctacagacat atacttccag aaaacacgtt cagagcctct ttcatcagga
     1561 ttaagtacaa caaagagtat tccggggtct aaggtgacat ccatagccat cgttgcagga
     1621 aaaccaagac tttgcaatga atatttgatt ttcccattat cctctctgta tgaatgagat
     1681 ccttttatct gaacaaagaa attttgctta ggtcgccttg atacatcagg atctgctaca
     1741 tattcaaacg ttccatcagt attaggccac ttatcattag atgcaaataa tgggttaatc
     1801 cgcccatttg aacgaaaaaa gaattctaat gtagagacag cagcttcgtc ttctgctgat
     1861 ctttcagtat gcgtttttat gtgcttttct attagttctt tgggctgctc taacattttt
     1921 tactcctttc tctgcctaag gcaaacaatc gcatcattca accttattat aataatctag
     1981 tcttaaatca ttaagttctt ttcgtacagc ccgccaatcc ttcatgtaca aatccataca
     2041 tgaaataaaa actgaattat gtgtacgttc ctttaaatgg agaagttcat gcaatattac
     2101 atactctaaa cactcgattg gtttctgtgc aagctgtaaa ttaaaccata gttttttctt
     2161 ctctgtatta caagttcccc agcgagttaa catatatttg gtttgccaag aatcacagta
     2221 tagctcggta agcctttccc acttcggaag taatctttca atttccgctt ttagtaagat
     2281 acggtactgc tctcgcacat agttttctct ttgctttact gtactttctt cacgcataga
     2341 gagtattacc ttatctcctt ttatttcaaa tccgttcttt tgtgaattag gctgaaagga
     2401 tagataatat tgttttcccc atatgaaaag cgtttctccg cttacatact gacgtttagt
     2461 gcttcttgcc tgatgttgaa acttttcaat ctgttttttg atccaaataa gatttgttcg
     2521 ggcatatatt tctattacct tatcatcaat agatgacgga gcagagataa cgacgtgtcc
     2581 atcaggaggc ttgacctgaa gatgcatatt cttgatattc tttttatgga tatcaatagg
     2641 tatgccagaa atcataattg tcatatatta gtattcctcc tgcttttcga tgattttaaa
     2701 taatctttcg acttcagaat catcattaag nnnnnnnnnn acggaatcca ctcattttac
     2761 tgctaagaac agcattatga agtttgattg ctaatatttc attaccgtca gtattatcgt
     2821 atagagcttg caaagcctta ctttttctta tgctttcagg atagtcttca ttttgttccg
     2881 gatgttcaac atttttagca agttttattt gttcctcaag gagttcctta taagagataa
     2941 cgccttgctt tcttttttga atcagctcat ccagtattgt tgacatcttt tcataatact
     3001 tagggttgac cgcaactttt tctaccattt tacgacgaat attattttca attgcttcag
     3061 ctgctccttc ttttttatct gagggggcat cttcttcggt catgatttct ccctgttctg
     3121 ctacgaaatc aagtagtgtt agatcatcga actcaccgat tttaacgcta tctgaagctg
     3181 taatatagtt gtcgatcagt ttgcgcatat ccggctcata agctttaaaa tccaagaagt
     3241 ctccactttt atttccgatt gtctgtttca gctcaacata gaattttact ttttcatcgt
     3301 atgcagatac ttctttggca gtataaatat caaccatgta tggcttggct tctgcaaatg
     3361 cacgaactaa accgcttaca agcttataaa gcctctctct aaggcgagaa tagatctcat
     3421 cagtttcttc gctcatgccg gattctccgc agaaataatg catatactgt aaatcaccct
     3481 ttggagcttc tacgccttcg catagttctt ccaactgatc gtacacttca ttgaaatgct
     3541 tgattgcttc agcgcttcta tctttgataa gtccatcgac atcttccgga tcatagtttt
     3601 caaatgcacc tgatgtatag gtgtccatag cattctttag gtctccgaaa agctgcttat
     3661 aatcaacgat atatccaaat tcctttgtgt caccatccag tctgttaaca cggcaaatag
     3721 cttggaacaa accgtgatcc tgcatactct tgtcaatata cagatatgta cacggcggag
     3781 catcaaatcc tgtgagcagc ttatcaacca caatgagcag tttcatgttt gcaggctggt
     3841 tgataaactt atctttaaca tctttttcaa agtcctcgac ttttttctgt attgaaagct
     3901 tttcaggaag attatcagga tcaagtccta acatacggag atatatctcg tatttttcaa
     3961 aagtttcagt ttcatcatcg gcacttacag tatcagttct cagatcaccg gcttgcggag
     4021 tgtaagagga gataatcgca cacttcttga aacctctctg ctggaagatc tcataatact
     4081 tgcaagcagt atagatcgaa tctgaaacga gaattgcatt tccatttccg tccattaggc
     4141 gaggcttcaa atcaaaatcc tgaattatat cccaagcgac acgttcaagc cttgaacggg
     4201 aactatagat tttctgcata cttgcccatt tctctgtcag ctttgcttta gctctgcttg
     4261 aaagagaacg tgttttaacg tcaaaccatt ggtcaactct gtcatgagcg ctaagctcct
     4321 gggggatatc tctgtattca tatctcagat caagcacaac accgtcacgc acagcttcgt
     4381 tatacttata ggtatgaata taagtgccaa acacttctat gcttgtcttc ttatcttttt
     4441 tcagaagcgg tgttcctgta aaaccgatga atatcgcatc aggcatgatt gcctgcattg
     4501 ctgcgtggag cttgcctgac tgcgttctgt gacattcatc aacaaagaca aagatttttc
     4561 ctttcgcctt aaagtcagca gggagcgact tcttcaactc atcaatatac ttgtcataat
     4621 cggattcagt agcttctccg ccacgacgac caaatttatg caccaatgaa cataagagtg
     4681 aactgtcata ggaattcaac ttacggagca gatcatcaca gctcttggtt ctggtgatct
     4741 tttcatcaac accaatgtat gtcttttcaa tctgctcatc cagttcatca cggtcggtga
     4801 cgataagcac acgagagttt tcttctttac aattggcaag gatccatttt gacagccata
     4861 ccattgtcaa cgttttaccg ctgccttgag tatgccatag aataccgcca cgctgttttg
     4921 ctaatctgtt ctgagtacgt tttatgccga aaaactgatt atagcggcat accttcttaa
     4981 tgcccttatc gaaaactaca aaattctcaa tgaggtctat aaagcgtttc ttatcgaaca
     5041 tctgatacag ctgctgaagc aggaggttat caatatgatc gcagtattcc ataatacgtg
     5101 catcttcagg atcacgctcg tcttcgtttt catggaatcc gtcaggcttc cattccatat
     5161 agtatttttc gcctgtcaga atagtgccgt aacgcagacc ttctatttca ttgcctgcca
     5221 tacaaaactg cactgtggta aagaacgatg caataaagcc gtccttctga ttggtaaggt
     5281 tctgcctgat accatttgaa acggacacgc tgctcttttt cagctctata actgccatgg
     5341 cgatgccgtt gacataaata acaagatctg gtcttttctc ctgacggtca ataaccgtta
     5401 cttcctcggc tattgcaaaa tcattatttg tagggttatc aacatcaatg aagtaaaccg
     5461 tcttagggga gctttcggga gtttccttga ccttggcacc gtatttcagc agcgtataga
     5521 ccttatggtt agcatcataa agcccatgtg tcatatcatc agcagctttc acaagttcgg
     5581 caacagcacc gtctatcagc ctttcactgt aaccgctaag gcgcaggtac gcacgaagtc
     5641 ggtcttcacg gatattttta tttgctgtgt ctttcaggtt gccaatatat ttataatcga
     5701 ggatatttct gttcttaaag aaatcaatta cacgattttg cgtggttctc tcggtatcac
     5761 cgatactcat acgtacattc ctccttaatg tgtttgttat ttcatggaaa aaactttctg
     5821 atcaatcctg cttttatgct tttatattta tcacacttac gctggtgaag ggtgataagg
     5881 gtgtcaagtt gagagaaaaa ggttccgatt ttttgctgtt catctttttg agtaggtaat
     5941 aaaataggtg tctttttccc gctttcgata gtataagttc cgactgttcc tattccagca
     6001 gattctttta taaagttctg aatgaaacta ccgttaaaag aatgatagaa aaattcgcca
     6061 tctattctat cttcattttt gaaccaaata atatttccat ccttgaagta tagcggttct
     6121 tcgttgttta tcaacattgg aacgcctatt gttccaacac ccgttacaag caaatcacca
     6181 attttcactt tgcccgataa agcactgtac tcatcatatt tctcttgtga tataaataac
     6241 ggttcagaaa cttcttcgtt ttttgatact gctacaatat ctcttgctct taaaaatctt
     6301 acacctgaat ctgtccaatc ggactgatga attcgtttta cagatgttat ttccatcagc
     6361 tcacccaact tacgttgttc ccaagcgtca gtaaatcctt tgaatcttag ttcaggctta
     6421 tcagcaccat tttggggaaa catttttttc agcatagccg ctttgaattg aatcaacttg
     6481 tcacacttac gctgatgaag ggtgataagg gtgtcgagct ttgaaaaata ctctccgatt
     6541 ttttcttgtt cctcatattt cggataaagc aaaactacat tttccaaatc cgaattatga
     6601 atatgaacaa cagattttcc ctgcgctctt tttgaaagct ctttttgctg atttccgttt
     6661 gaaatcgtca gggcaaggaa aacaggattg atataatcgt caggaacaat aatattcaaa
     6721 tcgccaccaa gcaaaatgcc cgattttgcg acaaatgaag cccttgaaat atcttctgcc
     6781 gtttcgccag atgcaggcac gataacttcg tgtccagtgc tataaacaga attaggctga
     6841 acatctacga aagtatcgac ctcgctgata acagtttcat actttgtgta gagcctacca
     6901 taaagaatta tgggagtgcc attttctttc aaatcgttct ttgaatatcc tgatcctttg
     6961 gaaaatgtcg cagcggtcct taactcacgc tgttcccaag tgtcagtaaa tcctttgaat
     7021 cttatcgccg gagttttgtt attattcgac atcgtttgaa cctccgagca attctattaa
     7081 ctctgcaaaa gctttcatat cataatcatt gcctgttaat tcaccaagca tatctgacag
     7141 tgatgattct gcttcgttta tctgaccgtc aaggtctgta agtgtaagct tatatctttt
     7201 tgaaagctca gtgatacgct cggcaagctt atgagaaatt gctgtgtaca gatcaattat
     7261 tcccttgcct atggcataat accacttctt gtctacaagc agttccttga tctcatcatc
     7321 ggtaagtgac gcatatctct cacgagcctt ttcatcaagt gccttatcca tttcttttat
     7381 aagctttgta gactcctcag acctttgtgc aagctcaaac gctttcttta actcttcata
     7441 gtcatcggcg tatgcagcag gtgcaggcgc ttctgcacgg gctgcactta aagcattatt
     7501 tatggatgtt ttggttactg ttcctttatc ggtatacgca acttccagtt cagcgtgctt
     7561 gtcgatatac tgtgtatatt ccttcttttt cattgacgga agaagattca aaagcgttac
     7621 aaggctgtca ataagcggag tatggacatt ttccatgatc ttgtcgattt tctcctgaat
     7681 gtccttgctc ttgacttttc caccctctgc aagttccgat aatgccgagt cttctgccga
     7741 tttctcgatc attgccatga gtcttgaatc ggtctctgcg acaatatcca taaggtcatc
     7801 tatcgccttt ttctcctcgg ggaatagctc tgagataact attttcttcg gaataagctt
     7861 accttcccag cctgcaacct ttagctcagg gttgccgtca gcatcggttt tctttgtctc
     7921 cttcatgatg ttctcggtct ctcttgcaat ttcatagccc ttatcatccg agattatcaa
     7981 agaaacatcg tcattaagct cttcgttcca gtatgcaaga agtatctgga aaatatcatc
     8041 cgtgttgatt agcaagatat cttcaaatgc tttcattata ttctctgcca actcggatat
     8101 gagaattttg gcagaaacgg agctatcaag ttttttcaac tttgtatctg caaaagattt
     8161 ccagtctgtg aatgccttgt cgataagatc cccatatgcc gagaattcac cattagcata
     8221 tatcattctg cgaatgtcgt tttcttccac attcagctta taatagccat cactaagcga
     8281 actgagcaac tcatctttca gcgtcgggaa tgcatcccag tatttactca gtgcatctat
     8341 atccacagca gggataccgc cgtgaatatg agcataaata tcctgtatat cctcaggctc
     8401 ggaagaatct atataccggg aaatattcag gttatagcca ttcttctctt tgatctcctt
     8461 attgggaaca aagcgagcat atttaggatc atctgtaatc tgctcattga atgtagtgac
     8521 tatgcgatag atatcctgtt cacgcagtct gttcttgttt ccgtccttaa caaatccctt
     8581 tcctgcatct atcataaaga ttccggaacg gttttcagca ccctctttat cgattataat
     8641 aatacaagca ggtattcccg taccgtaaaa caggttaggc ggcagactga taatgccttt
     8701 gatccagccc ttatcaacga tagcctgacg aatagtacct tcagcatttc cacggaacag
     8761 aacaccatga gggagaatga cagcagcttt acccgtagat ttcagcgttt ttaatatgtg
     8821 catcaaccag gcatagtcgc cgtttttctc aggtggtcta tctccataac cggagaacct
     8881 tccatactcc ttcaaaccat ctgaccagtt tttaagagaa aaagggggat tcgcaacaat
     8941 atagtcgaag cgttccagtt cggaagaatc ttcatttttg ataaactgag gatcagaaaa
     9001 agtattacct gccatgatct tgatagtagc tttattatga agaacggcat tcattttagc
     9061 aagacctgcc gtcgaactct ctttttcctg tccataacct gatatctcaa aaggaacctc
     9121 tgcaagtgct ctgataagta atgaaccact accacaagca ggatcacata ctgtggcatc
     9181 atgatccgta catttatcta tgcctataac ctttgcaaga atgcgtgata cttctgccgg
     9241 ggtatagaac tgccccttac ttttaccgct ttcggtggca aagtttctca taaggtattc
     9301 ataagcatcg ccaatgatat cgtcaccgtc agctttatta tttgagaaat ccaattcagg
     9361 acgctgaaaa atggctataa gctttgtgag cttatcgacc atttcctgtc ctttaccgat
     9421 ttttgcttca tcattgaaat gtgcgatgtc aattacacct tttagactgt catttacttc
     9481 agcaaggcga gcgatgattt tatctatacc ttcgccaata ttcttcttgt tcttgagtgc
     9541 aataaagtca tcaaaagaac agcctattct tttttcggga tcaggattgg gatcatgttc
     9601 tttatcgaaa acggtcaaat ctccgtattt atgtccctta tacttatccg ttacatactt
     9661 cataaacaaa agggttagta tgtaatcctt atactgtgat gcatccattc ctccgcgcag
     9721 tgcatcacaa cttgcccata gtgaactata taattccgtt ttcttaaccg ccataatacg
     9781 aatctcctta caattaagtt gctatatttc ctactcaata gcacttttgc cgctttttac
     9841 ccatgcatca agctccgagc gtttgaattt ccactgctta ccgattttgt gggcagggat
     9901 atctttatta ttgcgaatcc aacttcggat agtaacaggc ttgattccta agtgttcagc
     9961 tgcctcttca aggcttatcc atttttcatt aacagcgcta tttcccattt tatcacctct
    10021 tgtagtagat gaaatatata acaatccatt atgattatac tatatcggtt tgaaaattac
    10081 aagaggttta gtgttatttc gtgatattta tactaagttt atgtatatgc attgtgcatt
    10141 tttcttgtcg tatagaacga ggctctcaga atcactctga aagcctcgtt tcgtatttat
    10201 agatatcaga acggatgctg tatctgacag ccgcagtgag ccattttcgt tcgcctgaga
    10261 gcataccacc tgcactatca gaaaatttgc tccgaattaa cgtccacctt aacagcaaac
    10321 ttattctgaa agtgcagttc ctccacgatg agaacagcgg tgttatcggg agtgttcctg
    10381 agtgaaccaa caaaggtaat cgtaccgcct ttggaataga gaaaactcac agcacttgca
    10441 ttatcatcaa aagcgaggca ggtgaaagta tcgctctcga catcctcaac gcctttctgc
    10501 ttaggctgct caacggcgat actgaattta cagtatttag ggtcatcttt ggtagtaaca
    10561 gcaggctcgc ttagaagcct gcatgacatc atgatcttat tcaacatagt ctgctccttt
    10621 caatcttcgt ccgaacactc gttaagcccg tgtataagct gaatgtatac gcactctgct
    10681 cggtcgtgct cctcgccgtc cgtggacatc attatttcaa ggaaatatgc cttagcctgt
    10741 tcatagtccg tccagacttc acgcttgcca ttgcatacgg tagtgacctt gtttgttctc
    10801 ggcattgcca attctggtgt tttcaacata gctgtgtacc tccataattt ttatcgtgaa
    10861 tccattatat ccgataaaac taccggagtc cagcatatta gaatgattgt aataattctt
    10921 gtgaaaggcg caacaaaagt atgtttttcc ccagtgcatt gcgctgtgat gctaattgac
    10981 tttttcgagt atgtgtgcta tgatatttga aaagaattct agtaaatgct aaatcagcag
    11041 acatttatgt attgattaga aagaattatc ggaggttttt atatgagcaa caccgttgat
    11101 gtatattttt tatttaaaat catgcc
//
LOCUS       DAWEMR010000080        10950 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2527_length_10950_cov_12.052409,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000080 DAWEMR010000000
VERSION     DAWEMR010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10950)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10950
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_10950_cov_12.052409"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3231 a         2266 c         2691 g         2762 t
ORIGIN      
        1 gctccatatc atgtcctctc aaatgccgta aaaaacggcg gcaaataata tgccgccgca
       61 gtgttatcga tatttatcag ccgctaagtt ccatttcgtt agcgtcaggc ttgtaaacac
      121 gctgaatgtc agctccaagc cctctgagct tctcatctat cttttcatat cctctttcga
      181 tgtagaaaat attgtctatc tcagtcgttc cctcagccgc aagaccggca atgaccattg
      241 ccgcacctgc tctcaggtca gttgccacaa caggagcacc tttgagcttg ccaacgccct
      301 gtataacagc caccttgccg tcaacagaaa tgtcagcgcc cattcttcta agctcagcca
      361 catatctgaa tctgttgtca aaaatatcgt ctgtaacgat gctcgtaccc tctgcaagcg
      421 tcagcagtgt tgagaactgc ggctgcatat cagtagggaa gccagggtgt ggcatagttt
      481 ttatagaagt tttcacgagc ggaccgtcca cccaaacatg aacgctctcg tcgctttcct
      541 caacgtttac gcctaccttg cggagctttg ccgtgataga ctcaaggtgt ttaggaataa
      601 cgtttgcgat aacaacgtcg ccctttgtgg cagccgccgc taccatataa gtgcctgcct
      661 ctatctggtc agggatagtc gcataggtaa cgcctttgag gtaatcaacg cctcttatct
      721 tgataacgtc tgtgcctgcg cctctgatat cagcacccat tgagttaagg aagttcgcaa
      781 ggtcaacgat atgaggctct ttcgccgcat tttcgattat agtaagcccc tttgccttta
      841 cagcggcaaa tattgcattc atagttgcgc caactgtaac aaagtcaaag tatatctgat
      901 tgcccacaag ctcgtctgct gtgacatgat tagctccgtt cactatctcg tcagacgcac
      961 caagcgcctt aaaagcttta aggtgctgat ctataggtct gtcgccaagg tcacagccgc
     1021 ccggcatcgg aacaaaagcc tcgtgaaatc ttccaagcag tgtgccaaga aaatagcatg
     1081 acgctctcat ggttcttgca agttcatatg gcacttcagg cttttcaatg ccctttgtgt
     1141 caaaatatat agtattttta tctaaaagtc tgacctctgc gcccatttca cgcaggatct
     1201 tcatgcactt agttatatcg cttatctcag gcacgttctc aagaacgcac ggctcgtcgc
     1261 acaggatagt tgccgcaacc aatgcaacag cagcgttttt tgcaccgctt atctgcacag
     1321 taccatgcag cggcttacct ccatgtataa caaacttctc caagtttaca cactccttaa
     1381 cgagattgtc ggcttctctc tctgccgacg ctcagattat tataccacag ccacaggcaa
     1441 aataaaagca aaaagcaaga atttcaacga tttggttaaa atcatttaac atacacccaa
     1501 gcaatcgtta aatctgccca tataatgcag gctatggcgg tctataaaaa gaacaaatgt
     1561 tcacatactc ttaacaatag aatatccaac tcttatattt taacacaccc cattcaaaaa
     1621 atcaatagtg aataaggata ttttgataaa ttttcgacaa ttttttcggc tttttcggtg
     1681 aaaattacag caatgttaca actgtacgaa atcttgtacc ttattgtaac cttgctgtaa
     1741 tatttaagta caaaaaatca gacacttgcc gtttgtcttg acgaaaaaga acggctgtgg
     1801 tatactcata acaacagata tactgtaaaa aggggagtgc ggcagtggta aaaagaaaga
     1861 atctcatatg ggcggcagtt tttgcggctg ttatcgctgc gtgcctgata tttattttcc
     1921 tcacaggtgg aaagagcgga gaaaattctg ccgagatata tgaaaacggc aagctaataa
     1981 agactgtttc cgacctttcc cccgataagg agtattcctt tgagataaaa acagaaaacg
     2041 gcactaatac cataaccgtt ggcgagggaa gcatttgggt gtctgtcgca gattgctcga
     2101 accagacctg tgttcatgca ggaaagatat cccacgcagg acagaccgtc atttgtgcac
     2161 cgcacaagct agtgataaaa atagtcggtg agacctctgc tgacgctgtg atataggggt
     2221 ggaagcatga agatatcagt acaaaaaatc acccttatag gaatttttac cgccttgtca
     2281 cttatcattt tcatggtgga ggcacaattc cctgtcatgc ccataggcgg catgaagctt
     2341 gggcttgcaa atatcgtcac aatggcagca atgcttttta tcggcagaaa agaggcaggc
     2401 ttggtgctgc ttttgaggat agtccttgcg gcaggacttt acggaactgt ggtgagcttt
     2461 gcgttttcac tcacaggcgg aattttagcg tatctcgcca tgtacattgc ggtttcgaga
     2521 gttggtgaaa accagctttg ggtgacatca gtttttggtg gctttttcca taacataggt
     2581 cagctttgtg cggcggcagt ggtatcaggc acatgggcgg tgttcagcct tttgccttat
     2641 ctcataataa gcggcattct cacaggcacg ctcaatggtc ttatccttca aagattgtgg
     2701 aagtcgccac ttagaaaata cagcataaaa agataacagc ggcgtcaggg gcatacctga
     2761 tgaccgctgt tttgttctat agtcagttta gccaaaaaca gacttttcac aaagtggcat
     2821 ttctacattt ttgccttgtg agaaataggt gaaaactcag cgttttgctt gcatttgacc
     2881 gaaaaatatg gtataattag ttgttataat agggtcatta taggatagcg ttgaaattga
     2941 acggaggagt ggatttgaga atacttggta tcgacccggg ctacgccatt gtgggattcg
     3001 gagtgcttga atacaacagc ggaaaattca ctgttgttga ctacggtgca ataaccactt
     3061 cttcggatat ggatttcaac agacggctga aagtcatcta tgatgatatg accgagataa
     3121 taaccaccta taagcctgat gaaatgggaa tagaaaagct ttttttcaat accaatgaaa
     3181 agactgccat tgacgttgca caggcaaggg gagtgacact gcttcctgcc ataataagaa
     3241 atattcccat ctatgaatat acgcctttgc aggtgaaatg ctccatagtc ggctatggac
     3301 gtgccgagaa aaagcaggtg caggaaatga caaagaccat gcttcatctt aaagctatcc
     3361 caaagcctga cgatacagcg gacgcagtgg ctatcgctat aactcacggg ctttcggtga
     3421 acacaaggcg tgttatgaaa aattttgagg agaaataata tgatatattc acttagcgga
     3481 aagctcgttc ataccgagag cgaccttgcc gtaatagaat gtgcaggcgt gggctatgcc
     3541 tgcaaaacta ctttttcaac cttgcagcag atagcaggaa aaagcgaggt caagctttac
     3601 acacatcttg ccgtaaagga ggacgcggtg gagcttttcg gctttgcaac taaggaggag
     3661 cttaaaagct tccgtatgct catatctgtc agcggagtag gcccgaaggc aggtcttgcc
     3721 atactttcag cctgcactcc gtcacagttt gcgcttgcgg ttgcaacggg ggatacaaag
     3781 gctttcacaa agataaaggg tatcggtaca aagatagcac aaaggatagt cctcgagctt
     3841 aaggataagg tggcaaaaga gaataccata tccgtcagag gaaatgcggc tcagggcttt
     3901 gcggctgttc caagcggagc tgtcgaagaa gctgtcacag cccttgtggt gcttggatat
     3961 accgagggcg aagcaatgtc agttatatca aagcttgacc cgaatttgag cgttgaagag
     4021 cttataaaga aagcccttat cggacttgcg aaattttgag aggagaactc cataaatgat
     4081 cgaaaaagga gattttgata tagacaggat agtcaccccg aaagaaactc aggcggaaac
     4141 aactgacgat ttcgaggtga gccttagacc taaaacactt gacgagtata tcggtcagga
     4201 aaaggtcaag gagaacctga aaatatatat acaagcggca aaaaacaggg gcgacagcct
     4261 tgaccatgtt ctgctttacg gccctcctgg tctgggcaag accactcttt ctgccataat
     4321 cgctcacgag atgggagtga acatcaggat aacctcaggt cctgctatag aaaagccggg
     4381 cgaccttgca gctttgctca ccaaccttga aaaaggcgac gtgcttttta tagacgagat
     4441 ccaccgcctt tcaagacagg tggaggaagt tctttatcct gcccttgaag attatgcctt
     4501 ggatataatc atgggcaaag gtcctgcagc aaggtctata agaatcgagc ttaacaagtt
     4561 cactcttata ggcgctacca caagagcagg ctcgctttct gcaccgctga gagatcgttt
     4621 tggcgttatc cagcgtttgg agctttacaa tacagaacag ctttccgata tcgtaaagcg
     4681 ttccgcagtt ttgcttggcg tggcttgtga tgatgacggt gcagaggaga tagcgaagcg
     4741 ttcaagagga acgccgagaa tagcaaaccg ctttttaagg cgtgtgcgtg attttgccga
     4801 agtcatgggc aacggcagga taaccgcaga cattgcaaag atagccctca acagaatgga
     4861 cgttgacaaa ctgggacttg actctctcga caaacgtctg ctgacaatga taataaaagg
     4921 ctatgacggt ggcccagtgg gacttgaaac ccttgcggcg gctatcggcg aggagtctgt
     4981 caccattgag gacgtttgcg agccgtatct tatgcagctt ggcttcctct ccaagacccc
     5041 aaggggacgt gtggcgacac agcttgctta cgatcacttg ggttttctca aagacggaca
     5101 aacgcatctg tgatttagtg aggaatgagg aattatggta tcgcctagcg gcgattgatt
     5161 tcttgttctg taggggcgaa cagtgttcgc ccgccactgc actttctatc tttggaaaaa
     5221 ttttattatc ggatcaattt aatatgtgtg ttaatataga gtttacataa ataaaaaata
     5281 atatagaggt atgacctcga aaaggagcta attattatgg gaagattatt tggaactgac
     5341 ggcgcaagag gagtagctat caccgagctt acctgcgagc ttgctatgca gataggcaga
     5401 gctgccgcaa tggtactcac gaaggaaaca aaccacaagg ctaagataat cataggcaag
     5461 gatacccgta tctcaggcga cattctggag tccgcactgg tcgctggtat ctgctcagtc
     5521 ggtgcagacg ctcatatact cggtgttgtt cctacccatg ccgttgcaat gcttgtaaag
     5581 aaatacaacg cagacgcagg cgttatgata tccgcttcac acaattctgt tgagtttaac
     5641 ggcataaagc ttttctctgg cacaggcttc aagctttctg acgagataga aaacgagata
     5701 gaagcactta tcctggacac acctgaaaag atagagctta aggatggctg tgatataggt
     5761 cacgtttatt acgaaaagga cgcagcttgg gattatatcc gctacgttat gaaaacagtt
     5821 cagacagatt tcaacggtat ccgtgtggcg cttgattgtg caaatggctc agcttccgtg
     5881 tgcgcacaga gaattttcga gggacttggt gcaaagtgca taatgctcaa cgataagcct
     5941 gacggaacga atataaataa ggactgcggc tcaacacata tcgagggtct gcaaaaggca
     6001 gttgttgata atcattgcga tatcggtctt gcctttgacg gcgacgctga cagatgtctt
     6061 gcagttgacg aaaagggcga gcttatcgac ggtgataaga tacttgctat cttctcaaaa
     6121 tatatgaaga acatgggtat cctcaaaaat aatacctgcg ttgttaccgt tatgacgaac
     6181 ctcggtttct tcaagtatgc aaaggcaaac ggcatcgttg ccgctaccac aaaggtgggc
     6241 gacagatacg ttcttgaaga aatgctcaag ggcggttata atataggcgg tgagcagtca
     6301 ggtcatatca ttcttcttga ctatgcaaac acaggcgacg gtgagctgac aggtacaaag
     6361 cttctgacag ttctcaaatg cacaggcgaa aaggcaagcg agcttgcttc ctgcatggag
     6421 tgctacccac aggtgcttgt gaacgttaag ataacagcgg acaaaaaggg tatgtgggat
     6481 aaagtccctg agataacaga cgctataaag atgtattccg aaaagcttgg tgacgagggc
     6541 aggatacttg tccgtgagtc aggcacagag cctctcgttc gtgttatgat agagggcaag
     6601 agaacaggca taatcacaga atatgcacac aagatagcgg agcttatcga aaaaatgtaa
     6661 ggtaagtttt atataaggat aaaaaatatg agaattgcag aaaactggaa agactataag
     6721 ataatagata cctcaagcgg agataagctt gaaagctggg gcggcaaggt gcttgtaaga
     6781 cctgatcctc agataatctg gaagtcaccg aagcgttctg acctttggac aaaggcagac
     6841 ggcatttatc accgttcctc aaagggaggt ggcgaatggg aatacagaaa gcgcctgcct
     6901 gaaagctgga acataggcta taagaatttg aagtttataa tcagaccaac gggcttcaag
     6961 cacacaggtc tttttcctga acaggctgtg aactgggact tcatggctga taaaataaga
     7021 aacgcagaca gagagataaa ggtgttaaac ctctttgcat acactggcgg agctaccctt
     7081 gcctgtgccg aagcaggtgc aagcgtttca cacgttgacg catcaaaggg catggtgcag
     7141 tgggcaagag agaatgccgc tgtgtcaggg ctttccgata agcctattcg ctggcttgta
     7201 gacgattgcg aaaagttcgt tcagcgtgag atacgccgtg gcaagaccta tgacgctatc
     7261 gttatggatc ctccgtccta cggcagagga cccggcggag agatatggaa gcttgaagat
     7321 tgtatctatg accttgtaaa gacctgctca ggcgttttga gcgacgagcc gcttttcttc
     7381 ctgctcaaca gctataccac aggactttca ccctcagtta tggcatatat cctgagagat
     7441 gtcctcggaa cgaggtttgg cggaaacgtc acggctgatg agataggatt gcctgtggag
     7501 gcaacaggcg gtgtgcttcc atgcggttca acggcaattt ggcagaaata gctttgctat
     7561 agtaaggaat ggtgttcgca tgaaatatac tgagaattat gtttatatgt acggccaaat
     7621 gatctccacc tgttcgtttc ttcttgacgg tgattttcca aaggcagacg gcacggctga
     7681 gataaaggaa aaatatcacc ttgtcggtgg tgagaccgga acagcggcgg ctgttctttg
     7741 cagtctgaat gtgcctgtaa agcttggcgg tactcacctt ggaagcggca atgaaaagct
     7801 tataaaggat tattttgcag acaagactgc tgacctttcg gagcttgtaa cagagggttt
     7861 cgagggcgtg acagactatg ttatcataga taaaaataca cgcacctgtt ttggcgagtg
     7921 ggataagctt tattcacgtc cggagccttt ttatgaacag ccttcggagg agtcagtgaa
     7981 aaattcagcc tgcgttgcgg ctgaccctta ttttggtgat aagatagctg agtattgcgt
     8041 aaaatatggc aagaaatacg tcaccataga ttgtgcctat gacagctata tacatgagca
     8101 ttgtgcagta aattgcatat ctcatcagca ccttgatgaa tattatccgg ataaaagcta
     8161 tgatgagctt ttcaggcttt atacagataa tacagacggg cttgtgatat tcacccttgg
     8221 tgaaaagggt gcgatgtatg gcagaaaggg tgctgtgcca aagatgtgcc ctgcatttaa
     8281 agtagacgta gtgtcaacgc ttggcgcagg ggacagcttt aaggcgggcg ttgtatatgg
     8341 gctttataaa ggtcttgatg acgatacatt ggtgaaaaca gcctgcgctg tggcaggtgc
     8401 ggcggtggaa aagttcccta tcgcacttta tccgccaaca gtaaaagata ttgaaaagct
     8461 tatgggcaat atgtaggggc aaacagcgtt cgcataccca tatattataa taaggtataa
     8521 accaaaaatt cagacaggag gaaaggttat gtcttttgat tttgatgaac ttaaacacaa
     8581 ggcaggaaat atgcttgaaa agggcaaaga aaaagctcac gaggctgctg aaagtgaaaa
     8641 ggggcagaag ataaagagca aagcaggaga gctgtttgaa aagggaaagg aaaaagccca
     8701 cgagttcgca gagagcgaaa cgggcaaaaa ggtaaagaac aaggctgaaa agctccttga
     8761 taaaggaata gacaaagccg aaaatgttta cgaaaagcat aaggaaagaa aggctgaaca
     8821 gaaggaacag cctggagata aggaataaac taaatttggt aaggaaagta aaatgagcag
     8881 ttttattgaa ataaagaatc tgcacttttc ctatgtgaac gaccttgaag agccacctgt
     8941 taaaacggag gtgcttaaag gcataaatct gaacatagaa aaaggcgaat tcgtggcagt
     9001 gcttggtcat aacggctcag gcaaatctac acttgcaaag tgcataaacg ctataaatct
     9061 tcctgagtcg ggggctgtct atgtgaacgg catggacact ttggacgaaa ataacctctt
     9121 gcctataaga gaaaaggtgg gcatggtatt tcaaaaccct gataaccaga tagttgcaac
     9181 tatcgtggag gaggacgtgg ctttcgccct tgaaaatatg ggcgtcgagc cgaatgagat
     9241 acgcaggcgt gttgacgagg ctcttaaaac cgtcggtatg tatgaataca gacttcatgc
     9301 acctcataag ctttcaggcg gacagaaaca gcgtgtggct atcgctggca taatcgcaat
     9361 gaagcctgat tgtatacttc ttgacgagcc taccgccatg cttgatcctc acggcaggga
     9421 cgaggtaatg aaaactgtca aaatgctcaa tgagcaggga gttactatcg tgcttatcac
     9481 ccattatatg gaggaagccg cacaggcaca gcgtgttgtt gtaatagacg gtggcgaggt
     9541 cataatggat aacgttccga agaagatatt ctcaaatgtg gaaaccatga aaaatttggg
     9601 acttgacgtg ccgcaggtga cagagctttg ctgggagctt aaaagggcag gctatgacat
     9661 ctccaccgag ataatccacg aggacgagtg cgttcagaag ctttatgaaa tgcttaaatg
     9721 atagggagta acaatggcag taattaaaac ggaaaacttg acttatgttt acggcgaggg
     9781 cacaccattt cgcaaaacag ccgtagacaa tgttaatata gagataaatg acggcgaaat
     9841 ggtgggtata ataggacata caggctcagg caagtctacc cttatccagc attttaacgg
     9901 tcttttgaag cccacgagcg gaagcgtcta tatagacgga gaaaggctgt gggacgataa
     9961 atcaaggctt cgtcctataa gatttaaggt gggccttgtg tttcagtatc ctgagtatca
    10021 gctctttgag gagacttgtt ataaggatat cgcctttggc cctaagaata tgggtatcaa
    10081 ggaagagctg atagacaagt atgttaaaga agccgcaaga atggtagggc taagccctga
    10141 gatacttgac aaatcgcctt tcgagctttc aggcggacag aaaaggcgtg tggctatagc
    10201 aggcgttatg gcaatgaacc caaaggtgct tatccttgac gagccagcct caggtcttga
    10261 ccctaagggc agagagcaga tacttggtct tataagagag tatcatgagc agatgaaaaa
    10321 cactgttttg ctggtatccc actctatgga ggatatcgca aagaacgttt caaagatact
    10381 cgtcatgaac gaatcgaagc ttttctgcta tgacgataca gtaaaggtgt tccaccgctc
    10441 tgaggagctt gtgtcaatgg gacttgcagt gccacagata acaagagtta taaacaggct
    10501 caaagccatg ggcatagata ttaaggacga tgtgtataca gttggctatg cgaaaaaggt
    10561 catccttgaa atgctggagc agaaaaagaa taagtaagca gcggactgcc tttgacccat
    10621 atgagcagag agcaggactg tgtaagttga gatagaaaat tggaggaata taccttgata
    10681 aaggatatta ctataggaca gtattttccc ggaaattctg tcattcaccg aatggacgca
    10741 cgaataaaaa taataatgac ggcggttttc atagtaatgc ttttcgccgc aaacagcgtg
    10801 tggggacttc ttgtgggcat agcttttact attctcactt ttatcatatc gaaaataccc
    10861 ggtaaactca tgctaaaaag cttgaagcct atcgtgccga taataatatt cacggcgata
    10921 ctcaatctgc tttttatcag aacgggcaaa
//
LOCUS       DAWEMR010000081        10908 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2539_length_10908_cov_11.304432,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000081 DAWEMR010000000
VERSION     DAWEMR010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10908)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2539_length_10908_cov_11.304432"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    878..887
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2510..2519
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3124 a         2115 c         2611 g         3038 t
ORIGIN      
        1 actgtgtagg ctgcaagata gtcaatgaat atgcaggcaa cgagcattac aggagaacgt
       61 ccccagccga aaagtccgtc tgtgataccc tgaatgaact gaatgagaga gaaaacaaat
      121 gccgctccaa gaccctttgc tacgccatgc ttaatgctga aaacggctat aggaagcatg
      181 gagagcagtg tgatagagcc accccaaggg agcttgtata ctctgataaa gctaagcact
      241 gttgccagtg ccaccattac tgcaccctcg accaatgtaa gtgtttttga atttttcata
      301 ttataccatt cctttctgcg tacaaaaaat gcgtatgcct gataaaaggg catacgcata
      361 gatatacaca ctattttcct acgctggcat tacccatatc aggtgcggtc agaacatcta
      421 tttgctcttt ctcagccgaa aaacattcag ctcccgtgtt gttttatgta ctattgttat
      481 tatactgctt ttttcgtggg ttttcaatag cttttctgat tttccccacc taaattgtat
      541 gaaaacataa atatctgtcg caaaaccgca ttttattatt agttttgtgc atctttgctg
      601 agtatctcct ctatgttctc aaaaataaat tcctgcgcct gctttggaaa aagtatcgtt
      661 ttgtagctgt ttccaagctg tgatattttt tcacgctcta ttattcctgc ttcaggggaa
      721 agtattgtag tttcaagaaa gcccctgccg tttgcaccgt cttttgcaag ataattttca
      781 cctatgagcc attcttttat ggcaagtttt atgcttctta tatcgccgtc tgtgccgagc
      841 tgatagaaaa tgctgtcgca tacttctgaa agggtcannn nnnnnnnata tattcctttg
      901 tgctttgacc gccaaccgca ttttgctttt gcagatagcc ctctgggata tatttataac
      961 ccgaagcagc gtcaggattt tccttgagat atttctgtat ctcagctaca aagtatcttc
     1021 cataacgttc ctgctttgac tttcccaccc ctgagatatt caaaagctcc gtctgcgtct
     1081 taggcagctt tatacacata tcacgcagtg aagcgtctgt aagaatgata tatgcaggca
     1141 gactcgcctg tgtcgcaagc ttgctccgca cagctctgag ccgtccgaaa agctccttgt
     1201 caaatgtcgt aacttcgtcc ctgccgatct ttgtgaccgt ttcgctcttt ggctttttct
     1261 ttggaaggcg tattgtaata gtctttttgt ccattaatat ctctgcagac cttctggtaa
     1321 gtgtaagcac agaaaaatcc ccgtgtgaag tctgtgtgat gtaatcatca gcttcaagaa
     1381 aagctatgag cctgcggata tatacctgcg tggagtcttt cattatgccg taggttgaaa
     1441 gagtggaaag gtcaaaacgc tttacctttt cggagtctga gcctttaagt atctgtgcta
     1501 tgacacctgc tccaaaagaa aggtttcgct ggtgaagtct ataaatgcag gaaagcactt
     1561 tttggcaatc tacagacacg tcaacttctt ctgtttctcc gaaatcaccc aaacagcctg
     1621 agcagttctg acaaggcttg tcctgcttct caccgaaata tccaaggata aactctctca
     1681 gacagccgtt tgtgttgcag taatcggtca tctttttcag acgcataaga tcacgtttgc
     1741 gtatcatttc aagagtgtca tcgtcaaggt cgggattgtc atgtgaattg tttataagaa
     1801 acttgttggt cgcaacgtcc tgaccgctgt agagcaaaat gcactttgct tcgtttccgt
     1861 cacgtcccgc tctgcctgcc tcctgataat agctttcaat attcttaggc atattgtaat
     1921 gtatgacata gctcacgttt gatttgtcta ttcccatgcc gaaagcattg gtcgccacca
     1981 tgacctggac acggtcaaaa ataaaatcat cttgattttt acgcctttcc tcatcagaaa
     2041 gtcctgcatg atagcgtgaa gcgttgtagc cgtcggcaca gagcttttca cacacgctct
     2101 caacattttt tcttgtggag cagtagatta ttccgcagtt gtccttgtat ttctcaacta
     2161 tcctcactgc ttcactgtat ttgtcatgag ggtgagaaac gccaaagtag agattttttc
     2221 tgtcaaagcc tgtggttatg gtgaaagggt cacggagttc aagcattttg ataatgtccg
     2281 cttttacttc tgcggtggca gtggcggtga atgcactgac tatgggacgg tagggaagtt
     2341 tgctaagaaa ctcgggtatc ttcatatagg aaggtctgaa atcctgtccc cactgagaaa
     2401 cgcagtgggc ttcgtccacc gcaagcatgg atatcttcac cgacaaagcc agtgacagaa
     2461 acgatcctgt catcagcctt tcaggtgcaa catatattat cttgtacatn nnnnnnnnnt
     2521 tgtacatacc acgggcggcg tttgatatag ccatttcata ctgtgccgcc gtcagtgacg
     2581 agtttagata cgctgccctc actcccgatt gtatcagcga gttcacctga tctttcataa
     2641 gggatatgag tggcgaaaca actatagtta tcccgtcaaa cataagagca ggtatttgat
     2701 agcacataga tttgcctgcg cctgttggca taactccaag gcagtcacgc cctgcaagta
     2761 tgctgtctat tatctgctcc tgaccgtttc tgaactgaga gtgtccgaaa aagtcctgaa
     2821 gcactgaata cttattgcga aaatctgtca ttttatccta actttccatt gtgtgtatgc
     2881 ttatattata ctaccaaaaa ccagctaagt caaacagata tacaaaattc catgcacgat
     2941 tttgtaacat cttttcctta gtcgctattg taattttatc gggattatgt tataataggc
     3001 ttgataaata attagttata aaaatataaa aaattaacaa atctccaaag gaagggttgt
     3061 ctgaacactg tcgtatattc aggcatggtg aagatcatgg atatggaaaa taaattgtgg
     3121 caaagctatt ttggtgctgt ctggggaaag gtttgttcaa tatggcacaa tatcggcggg
     3181 tttgttatgg acgagaaaag cggagagttt tatgccgatg aaaactgtcg tgctcttatg
     3241 ggacttagag agaacacaga ttatgacaag ttcagagata tagttgcatt gaacggcgga
     3301 ggacgagatc ttggcctgcc gttgtgtata gaacttcttg acactccaag tggcattact
     3361 gccggaatag tgtatctgtc gaaagaatat atgtcaaaaa gtgtttttga agggctcgac
     3421 attataccac aatcagagct tgtaagactt cttgatggaa tgacacgctc atcattgctt
     3481 gctcttgtaa gatttgatgg agcagggaag cttttggatt cagactactg cataggggag
     3541 gcacttaagg cggcttttgc ggttttgcct gaaaatactg tgacggcatt caattcagac
     3601 ggtcagtttt ggatatatgt tccacgcttt gtaggtaagc ctgaggagtt tgctgacaat
     3661 atacgtaaag cggtgaagga gtgttgcctg ccagatgaat ttggagtgag atcgtcttca
     3721 aatcattcgc tgtctgtgac ggcaggcata agcagcggct ttgaagtgcc agcaagactc
     3781 atgcacgcag caggctttgc actctatgaa gctaaggcaa agggtgcagg cagtatttgt
     3841 tgctttgatc ctgaaaaata tgcaaagcag aagagtgata tcgagagtat cagggctttt
     3901 tcagagcttc ttgataaaaa tctgtttaca tatcattttc agcctatcgt tagtgcttca
     3961 acaggcgaga tagtcgctta tgaagcgctt atgagaacaa agggaaatat agcactcaat
     4021 ccgcttcaga tactcaactg tgctaaaaat tttggcagac tttacgatat agagaaagct
     4081 acactgaaaa atactcttaa atatctcagc aagcatcagc gcgattttga aaaccgcagg
     4141 ctgtatataa attcaatatc atctcatgct cttgacgata aggattttta tgcgatcgtc
     4201 aatgactatg gcgaacttct tgaaaaggtc gttatcgaaa tgacagagca gacagagata
     4261 agcgaggacg acctcgatag aataagggtg cgtcttgaaa agaacaatat gagccttgct
     4321 attgacgatt atggcacagg ctactcaaat acatcgaatc ttctgcgata tgatcctgag
     4381 gtggtaaaga tagaccgttc cctcatttct ggtattgatc agaatcctaa ggctcagaag
     4441 atagtttcaa aaatggtgga atatttccat tcaagcggat ataccgcact tgcagagggt
     4501 gttgaaacaa gcgaagaact taaaaccatg atatatttcg gcgtcgatct tattcagggt
     4561 tattatgttt ctaagccgaa gcctgtgctt atccacgata tctcggaaaa tatccgtgag
     4621 gagatagtgg cttacagtat cgaggctgga gataaagata aaaaggtctt ccacgctgag
     4681 gataatgacg ttatcgacct tgctgagatg tataaaaaaa gatattctga catttttctc
     4741 ggcacaggta catttactct ttcaggaaag gctgaggacg atcgtgcagt gcctctgtct
     4801 gtgacagttg gtagtggcgc tgattgcgtc attcacttga aaaacgcatg gctcactaca
     4861 tatggggaac taccgaacat aaagcttggc actggctcga gagtaaggat agtgtgcagc
     4921 ggtgaagatc atatagacgg cagaggcatt tatgtccctg agggatcttc gttagagctt
     4981 gtaggctcag gtgagcttta tgtcagatca gagtcgaagg attgctatgc aataggcact
     5041 gacagcgggc agccttgcgg aaggataact gtcgcaatga caggtattct tgatataact
     5101 gcaaatggcg acaagtgcgt tggaataggc ggaggaggct gcaaagatgg tattgtcatt
     5161 gcaggcggtg atatagctgt aaattgcagt ggcgataggt gcgttggtat tggaagcatc
     5221 gacggcgacg cagatgtgac tatatcaaac tgtggctgca ggctaaagct tgcggcagga
     5281 atgtcagtgg gcgttggtgc tgtaaagggc agtgctgata taagcatttc agattataac
     5341 atgagttgtg agctttctgg aaataacctg actgcagtgg gagttatgag taacggaacc
     5401 ggaaggatat gtattcttga tggcagactc aacatttcca tgaagggaag aactctcaac
     5461 tgtgtgggaa caagggacgg cgagctggat tgtgagctta aaaatactgt ctttaagctt
     5521 tattgcgagg gtggttctgt ttcaggtgtt ggagataaaa caggtaaagg tgacgtgaca
     5581 gcacaaagct gtcagtttga cgtaatgttc cttacaggcg atggctggtg gcttggaagt
     5641 ccgaatggta cgctttcagt agtcgactgt aagaaagata tcaagataaa caaataaggc
     5701 aaagcccata ggcgtgcctt gtgtgacgct gctttagatc gtctgaagca gcacggctga
     5761 tttgtacgga ggatcatgag atgatgaatg atactgctaa acgcagacgt ataaaaatag
     5821 gacttttggt gagccacctt gaagatgatt ttgacgatgc ggttgcagag ggcgcaatga
     5881 taggcgcaga gcagtgcgac gccgacctcg ttatttttcc aggcagatat atagatgggg
     5941 tctatgccga taagctgaga acggtgtatg agtatcagta taatactttg ttcgatatgg
     6001 cggcggcaaa taagttcgac gttctgcttg tgcttatcgg cacacttgga acatatcttg
     6061 acaacgagca taaggttcag tttttgaaaa aatttgctgg aacgcctgtt ataactataa
     6121 cggcacatat tgatggttat ccttgtatca tgctcgacaa cagaacggga atgaaggagg
     6181 ctatagaaca tcttataaag gtgcatggct gtaaaaagat aggtatggta agcggaccta
     6241 aaacaagtga tgatgccctt gaaagacttg acgtttacaa ggagactctt gaggaaaatg
     6301 gtatcatcta tgacgaaaag cgtgttgcat atggtaattt ttcgaaattc tgtgttgacg
     6361 aagtcggaaa gctaattgac gataatccag aacttgatgc tatcgttttt gcaaacgacc
     6421 agatggcaat tgcaggctat aaggctatgg aagagcgtgg aatacgtcct ggtaaggata
     6481 tccttgtgac aggctttgat gacgatccag tggctgagga gcttaatccg catttgacga
     6541 ctgtcaaggc tgacccttct gagcttggct atcatgccgt tcaggaggct gtgaactatg
     6601 ttataaataa ggctataaac aacgataaga tctcttcaga aatggtaaga agaaattcct
     6661 gcggctgtca gggcaactca aagttaaaga ccatatcttt tggtcgcata tcagatgatc
     6721 cggaagcgtt tgcaagaaga atgagccagt ttttgttcaa taaatacagt gcaagtgaaa
     6781 caactgcgaa aatgcgagaa aattacgtca agataatata tgctcttgat gattatgccc
     6841 gtgaggaaaa tcttgataat gagatcaaaa aagacaaagt attagaaatg gtctcaattc
     6901 ttgccagtga agaatttttt aggtttgtga gcgttgatga gctttatgct tctgttgagt
     6961 atattcagca aaagcttatc tctcgttttg ataacgtgga gaagcatttg aagttaaatc
     7021 agacttttat tcagatatac aagatactgg cagaacgcaa tgcgaattac tgtaaggaaa
     7081 agcttgagga taatgagttt cttgcatggc aggcaaactc tatcacaagg gatatgcttg
     7141 tgtttgaagc ttatgatgac agagcatatg agaccgttgt ggataagctt acgaggcttc
     7201 atatggaatc aagctttctg ttcagcttcg agcctgccat tattcacttt ggtacagata
     7261 agtggcaacc gccggaatat atgtatctca aagcctatca caatggttct gacgctatcc
     7321 agctcccaca tgaagagcag gctgtaaagt atacagagct gttgtcgaat aagtatctgc
     7381 caacagacag acgttataca ttagtggtat caccattgtt ctctaacgag gagcattacg
     7441 gaatactgat gtgtgagata aagcatgagt attttaatta tttgcagtcg gtgacagttc
     7501 aactttgtgc ggcgcttaaa ataataacct tgatgaagca gcaggctgtt acccagaagc
     7561 agcttaaaaa gagccttatc gagatcaagg agaacaatga gcttcttcga gatctttcaa
     7621 ggcaggacgc tttgacggga tgctacaatc gaaggggatt ttttgaggag cttcgtaaaa
     7681 gactcagcaa tgacaaaaac gagggtgaaa aggctatcat ggtctttgcc gaccttgact
     7741 gtctgaaaac cataaatgat aaatttggtc acgaggaggg cgactttgct atactatctg
     7801 cggcaaagat acttaaacac tcttttggca acagtgagat agtgggtcgt ataggtggcg
     7861 atgagttcgt tgtatgcgct tttcttgata atgcaataga tatcccgtca atgagaaacc
     7921 atatagaagc ggtcacaaga gaatataata ttaaatatgc cgccgataag ccatatataa
     7981 tccatacgag cgtaggcgtt tatccgttcg tatgcagtgc cacgcttgag ataggagagc
     8041 ttctcagcca tgctgatatc ctgctttatg aggagaaaaa gcataagaga agtattctta
     8101 aaagcgatta tgaataacat ttgcaggctg tctgaaaaag gcagcctgct ttttgttttt
     8161 atgcttgaca aaatatcatg gctgtgatat aataaaacat attttacgaa aggagcgtcg
     8221 gatgacatga gaattacaag aatatgggtg agaaactatt aaaccgaata tcaggcaggt
     8281 gcattggcac gagccgctcc gactcttccg tataacgttt tggttacacg cctgagggtg
     8341 tgttttttta tgcctgaaaa agccgaaggg cggcttgata acagacatta ggagtgatta
     8401 atatggcaga aatgtttgac aacacaataa agaaagaccg tgtgctgctt gcggcagtgg
     8461 acacaggttc gtatgatgtg gagctttccc ttgacgagct tgaagagctg acagaaacag
     8521 caggcggtga ggtgatagcg agagtaacac agaaaaggcc gtcctttgac agcggtacct
     8581 gcataggctc aggcaggctt gaagaaatgg ctgagatatg cagaaatgaa aatattgaca
     8641 ggatagtttt cgactgcgaa cttacagcca cacagataag aaatatcgag gacgtgtgcg
     8701 gtgttttcac tatcgacaga actatgctca tactggatat ctttgctcag cgtgcgacta
     8761 ctcgtgaggg cagattgcag gtagagatag cacagaacaa atacagactt ccaaggcttg
     8821 caggcatggg aactaatatg tcacgtcttg gcggtggtat cggcacaaga ggtcctggtg
     8881 agtcgaagct tgaaactgac aagcgacaca tcagaacacg tattgcggca ctttcagacg
     8941 agcttaagga gatagagaag cgtcgtgggc ttatgagaaa acgtcgcaag aaagacggtg
     9001 ttctcacagc ggcgatagtg ggctatacaa acgtgggcaa atccacactg cttaattatc
     9061 ttacagaagc gggcgtgctt gcagagaaca agctttttgc gactttggaa acaacatcaa
     9121 gggctataga gcttcctgac ggcaggtcgg tgacgcttat tgacacggta ggacttatca
     9181 gacgtctgcc gcatcagctt gtggaagctt tcaaatccac tcttgaagag gcggcaagtg
     9241 cggacgttat aatccatgtg tgcgacgcct cggctgatga ctgcgaggaa caggcaaaag
     9301 ttacccttga actgctcaaa gagcttggct gtgagggaat acctgtggtg acagtgttca
     9361 acaagtgcga caaagtgcct tatataaacg agcttgacac atcaggtgaa gcggtgaaga
     9421 tatccgccaa aaacggcaca ggcattgaca gcctgcttgc ggctatacaa aaggcattac
     9481 ccgaaaacag cgtaagatgc aggcttcttt tgccttttga caaggcgggt cttgtgaaca
     9541 ccataaggca ggagggcagg atattcagcg aggattatac cgctgagggt atcgttcttg
     9601 acgctcttgt tgatattaag gtctatcatc ttgtggaaag ctataaggtg aaaagtgaag
     9661 aataaagtgt tttgcaaacg ttaaaaaaat gtaaactctc ggcagggcta ttgacaagcc
     9721 ctgcctgtga gagtataata atcgtggtta gcaataacaa ataaatcgaa aggaaaaaac
     9781 agaatatgtc tactgttatc atgtctgcag tctttacata cttctttatg agcttgagct
     9841 acttttatgg cttcgagttt ttttggcgtg tgaagaaagc gacgagatca gaataggcgt
     9901 atagagaagt ttctgttttt atatatgcgt ttattataag ccgtggcttt cttgtaaaag
     9961 aatgtcacgg ctgtttttat tgcctataca aatttgattt tcggaggaaa gtaacatgat
    10021 catcgtattg aaaaacaatt cacaggaaaa acaggtagaa gagcttttga actggctcga
    10081 ccagttccat gtatcgacga atctggtaga gggcgttcat tcaaatgtta tcggtcttat
    10141 tggcgacaca actcagatcg atatagaagc agttcaggct aaggaatgtg ttgaaaatgt
    10201 acagcgtgtt caggagcctt acaagaatgc taacagaaag ttccaccctg ataatactgt
    10261 tatcgacgtt gcaggcagaa agataggcgg cggaaagctt caggtcattg caggtccttg
    10321 ctctgttgaa actgaggagc agataatcac aactgctatc gctgtaaagg aagcaggtgc
    10381 aacaatgctc agaggtggcg cattcaagcc aagaacttca ccatattcat ttcagggtct
    10441 tggcaaggag ggcatcgacc ttcttattca ggctagaaag ataacaggtc tgcctatcgt
    10501 tactgagatc atggatcttt ctgatcttga ctgctttgct gacgttgacg ttgttcaggt
    10561 aggagcaaga aatatgcaga atttcacact gctcaaggct ctcggcagaa gcgacaagcc
    10621 tgttcttctc aagagaggac tttcatcaac acttcaggag cttctcatgt ccgctgagta
    10681 cataatgtct gagggcaatc agaacgttat cctttgcgaa agaggtatcc gtacattcga
    10741 gcctgccaca agaaatactc tcgatctttc agccgttcct ctgctccagc agaaaacaca
    10801 ccttcctatc actgtcgacc ctagccacgc tacaggtatc gcttcacttg ttgagccaat
    10861 ggcactttgc gctgtaacag caggctgtga cgcacttgag atagaggt
//
LOCUS       DAWEMR010000082        10761 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2580_length_10761_cov_10.279750,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000082 DAWEMR010000000
VERSION     DAWEMR010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10761)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2580_length_10761_cov_10.279750"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3287..3296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7949..7958
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10030..10039
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2688 a         2925 c         1964 g         3154 t
ORIGIN      
        1 aatttacaat ataagccgtc atgtctaata taatataatt tttgtttcat attcttatat
       61 taagtataaa atcttctgat tttaagcctt ggttcatacg aaaacaatgt taatatcttc
      121 atgttctaaa gagtagtata gcagagctta atacaaattt atagtgaaat aaacaaataa
      181 ataatgaatt ttcagtgaat ataaaaagcg aacaggcatc cacccgttcg ctttaattga
      241 tatgattaat ttacatctcc gagcttgtgt ctgcactgtc gaccgcactc tcagaaacct
      301 cagaactatc cgcactgcct gcaaaagcat tgctgttttt attctctccg taggtagaca
      361 tatacacctt tgtgaattgt atgtcctccc taggcttcag cgtctcagag tcagccacat
      421 cagtaccgca tatctcgtca taaatatcca agccatcata tacctgaccg aaaacagtat
      481 actgctgaga gaaattcagc acaccgccat atttctcaaa agcatcggtg atatcctcag
      541 ggatattgtt gctgtctgca agcttgctgt caagctccgc ctgctcctcc tcggtaaggt
      601 catagttatc aacaaaaaga agatagctgt ttgaaacctt cttcttgctg aacaagtgac
      661 cctgctcctg cgtgtatgca caaagcgcac cttttaaagg ccacaggttt tttgaatact
      721 ccgcctcgat ggtggtggtg tcagtgtcag aagttgtctg accgtcagcc tgctttgttc
      781 cgcctataaa atacacaccg tcctgcacca tgctcacata agtgccgtca taatagccct
      841 tttccacaag tccagtgaaa aacttgcaat agttcggtgc ttcatcaggg aaaagcacag
      901 ccttgaaagt tcccttgttt gtttcaaaaa ccaccacagg gctgtcatcg gcaggttcgt
      961 ccatctgtat cacctttgca tcagtatagt ctatatcgtt cttgttgctg ccagtgcagg
     1021 cgtaaaagag aacaaagatt ataacaaagc aaattgctat aagtgaaaac tgaaaatacg
     1081 tctgaaactg ttttctgcgg tatggaccca gatcctgttt atttttgctc atttaacgct
     1141 cctttacttt ttctttatct ttgtgccctg tcttgtttcc tcaacaatat agccaagctc
     1201 gcctatctta tctctcagct catcagcctt agcaaagtcc tttgcctttc ttgcagcctt
     1261 gcgctcctca acaagtgcct tgacctcctc agggatatcc tcttcaccgt cagcactgag
     1321 cttctttgca gcttctatca gaccaagtga aagcaccttg tcaaaatctt cgataagggc
     1381 aagctttgtt tcattgttag tgtcagcttt aagaacgtca tacagaacag tgatagcatt
     1441 tgaagtgttt atatcgtcat caagtgccgc cttgaagcta cccttgaact tctcaaaatc
     1501 ctcaagctcc accacctcgt caggcttctt gtcagccata agctgtgcta tccttgcagt
     1561 gagcttgttg tatgctgtct ttgcattgtc aaggttttcg tatgtgaaaa caagtgactt
     1621 tctgtagtgt gactgcaagc agaaaaatct gtataccagt gggtcatagc cctttgactc
     1681 cagcagtgaa acagtaagga actcgccctt agactttgac atctttccgc tgtttgtgtt
     1741 aaggtgcagg atatggaacc agtagttgca ccacttgtga ccaagataag cctcagactg
     1801 tgctatctcg tttgtgtggt gtgggaaagc gttgtcgata cctccgcagt gaatgtcaag
     1861 tctttcgcca agatgtttca ttgaaatgca agagcactcg atatgccagc cagggtagcc
     1921 cacaccccaa ggggaatccc acttaagctc ctgatcatcg aacttagact ttgtgaacca
     1981 cagtacaaag tcagccttgt tgcgcttgtt tgtgtcctcc tcaacgccct cacgaacgcc
     2041 gacctcaaga tcttcctcat taaagttgtg gaaaacataa tactctttca gctttgaagt
     2101 atcaaaatag atgtttccac ctgcctcata agcatagccc ttgtccataa gagcagatat
     2161 caccttgata aaatcaccaa tacagcccgt tgcaggctca acaacgtcag gtcttttgat
     2221 atttagcttc ttgcagtcct caaagaaagc gtcagtgtag aattttgcga tctccataac
     2281 agtcttatgc tcacgctttg cgcccttgag cattttatca tcgcctgtat cgccgtcaga
     2341 agtaagatga ccaacgtcag tgatgttcat tactctcttt acgtcataac cgtcatagcg
     2401 gagatacttt tccaaaacgt cctccattat atagcttctc agattgccga tatgggcata
     2461 gtgataaact gtaggaccgc aggtatacat tgacaccttg cccggctcgt ttggtacaaa
     2521 tttttcgatc ttgtgagtaa gcgtgttata aatgttcatt tatctttacc tctttcctta
     2581 tattttactg ctctttcttt ccaatctttt caagctcagc cactctcttt tccagcttag
     2641 ctatctgtat tcttgtctgg caaagctcct gagccacagg atcagggata tgcacctgat
     2701 cgaggtcaca ggtgcgcttg ccgttctgct taactatcct tgcaggcacg cccacagccg
     2761 ttgagtttgc aggcacaggc tcaagaacta ccgcatttgc ggctatcttt gcattgtccc
     2821 ccacagtgaa tggtccaagc acctttgcac cgcaccctac cataacgttg tttccaaggg
     2881 tcgggtgtct tttaccgtgg tctttacctg ttccgccaag ggtacagccc tgatacagca
     2941 ggcagtaatc gcctatctca gcggtctcac ctataacaac acccgagccg tggtcgataa
     3001 gcagaccctt gcctatctta gcacctgggt gtatctcgat acctgtcaac attcttgcaa
     3061 actgcgaaat acatcttgcg atgaccttgt gcttgtgaac atagaaccag tgcgacaccc
     3121 tgtaaaaagc cacagcgtga agccccgaat atgtgaggat ctccagcgta ctagttgccg
     3181 caggatctct gtcctttata gtctgaatat catgctttat gttttccaaa atcgtcatat
     3241 ctatctctcc ctttctaaga aaaaacctcc ggaatggcgt ttcaccnnnn nnnnnnccga
     3301 tttcctgtac aaaattttgt acagctcaat atctttaacg cagaccctac ggagaggaat
     3361 actaagcttt cgccgttgtt cgctccctgc tgtcagccgc acttcactga aagtccttga
     3421 agccttgcac caaacggctc ctctctgaaa attaccttac agctactctt gctgatacgc
     3481 atttacatat taagttatca tatgccgcaa cacgtcggca ggccacatta tttgcccgaa
     3541 tctatatact tccagtatcc cttgagataa actgcgccag aaatgactgt cagcacaaca
     3601 gcaatccata tgagtatctc gtttacgata aacacaggtg ttccccagtt aaggtcaggg
     3661 ctgccttttt ccgcaggcat gacgatctgc aaaaccatta tagcgataag agcgaccatg
     3721 gtgaaagcag ttttcagctt gccccatatt cctgcagcga ccacaacgcc ctcgctagcc
     3781 acaacaagtc taagaccagt taccataaac tctcttgcaa gtatcagtac caccgcaaca
     3841 gggtctatcc aacgttcaaa tgtgaagcag ataagcgccg tcataacaag aagcttgtct
     3901 gcaagtgggt caagaaactt tccgaatgtt gtaacaagat tgtgctttct tgctatcttg
     3961 ccgtcaaacc agtctgtgat agacgcagct gcgaacacga taagcgcaaa caccatgtga
     4021 tgcggtatct tgaaaagcag cacgaacagc agaaaaaacg gtatcaatat tactctaagc
     4081 gctgtaagtt tatttggcag gttcatatca agttcctttc atctacattt ttgagccgat
     4141 aaggtcataa tccatagtat cgtcaatttt taccttgaca tactcgccga ccctaacttt
     4201 atcctcgctc aggaagaata tctttccgtc tatctcaggt gcgtccgcct gacttctgcc
     4261 gtagtaacac tcggcatatc tgtcaaagcc ctcgcaaacc acctcgactt cgctgtcgag
     4321 agcagcctca ttaaggctgt cggaaataat catctgttct tccattatta tctccgctct
     4381 gcgctctctg tcctttaaag gcacctgatc ttcaaacttc tccgcaggcg taccctcctc
     4441 aggcgagtaa gcaaaacagc caagcctttc aaagcgaatt tcctttacaa actcacaaag
     4501 ctcctcgaac tgttcctcag tttcccctgg gaagcctgta ataagagttg ttctcagcgt
     4561 aacgttcggt atcttttctc ttatcttctt tataagcgcc gtaagggtct ctctgtcccc
     4621 atggcggttc attcttttaa gtatggtctt gtcgcagtgc tgtataggca tatcaagata
     4681 ctttacaagc ttgtcctctt tagcgataac gtccagcaat tcgtccgtta tcctctcagg
     4741 atagcaataa agcgttctta tccacttgaa gccgtctatc tcgcaaagag ccgtgagcaa
     4801 ttcaggaagc atagacttgc cgtatatatc ctcgccatag cgtgttgtat cctgcgccac
     4861 aactacaagc tccgtaacgc cgttctcagc cagccatctg gcttccttga cgatatcctc
     4921 catgcgtctg cttctgtatt ttcctcttat agacgggata gcgcagtaag tgcagttgtt
     4981 gctgcaaccc tcggctatct ttatatacgc atagaaaggc tcagtagaaa taattcttcc
     5041 gccctccatg gaaagctcag tttttgcgcc ataagaacag accgcctgct tatccagcag
     5101 tatctctctt atgatatcgc aaatttttcc gttagagcct attcccacaa cagcgtcaag
     5161 ctcagggatc tcctttttca tttcgtcacg gtatctttca gcaagacagc ccgtagctat
     5221 taccgccttt atcctgccct cctgcttgag agtacataac tcaagtatag tgtctatagc
     5281 ctcctgttta gccgactcga taaatccaca ggtatttata atgaccacat cagaaagggc
     5341 tgcgtcagaa actatctgaa aaccctcttt gcgcagatca tagagcatat gctcagcgtc
     5401 cacctgattt ttaggacatc cgagagaaac cattcccact ctagttgcca tgaaaaaatc
     5461 tccaatccta caccgccaac ggacggcata aagtatcaca ataatctatc ttattatctc
     5521 acttttacac agttttgtca agccctttta gtcactattg tatgacaaaa atcaaaatat
     5581 ttatgaattt tccacaaagc caaaagcata cggaaagtaa gtccgacatg atcccaaaag
     5641 aaatccccga aagctgcaac tttcgaggat ttcttttgta tgtcactgtg aacacaatca
     5701 atctcgtggt tacctatatc atacaccacg ccatatattt tgtcaagcac ttttgcgccg
     5761 tcagtctttc ttctcaggat atgtgaccgt tatctcagcc cctgcttcct gcgaccaaac
     5821 aacataatca agctttcctt catcaataaa aacagtgaaa tatgtatcct taagctcatc
     5881 aggaagctgt tcctttatct tttctatgaa cttatccgtc tctgtagaaa tgtagttcat
     5941 gtactcaaac ttgtaactct tgtcttttga aagctccgtg accgcctttt ctgccgactc
     6001 atataccgcc tgagcgttag cctctgccct ttccgagttt tcgtcagtga ttatgccgtc
     6061 agcgtaagcc tgcgcctttg accctgcaaa gcaaactttc caatcattgt ccttgtcaag
     6121 caccataaga atatactgcg atgacgtgac ctctccctca gagctgtcat aagctatggt
     6181 cacataaagc tccgccgcct ttgaatactc ctccgagatg ttcaccttgt cgataaagcc
     6241 gcacttatcc ttgtcaaagc tatatcttac tccgcttatc tgctcgtcct caccgtattc
     6301 attgtcgccg cccatgttct ttttcatata ttcatagtca gcggcataat cgtctgtcat
     6361 aagcttaacc attgattggt caaactcagc gtttgcggct ttaagataca ttgccgcaac
     6421 gtcctttgcc tcttcaagcg ggtcagcggc ttcttcgctt tccgtatcag ttccctgcgt
     6481 tgtcacagcg ttctcactgc cctgaacatt ttccgcaggc tgagagttat ctgtgccgtc
     6541 aaagctcagc gaacagcctg tcatgatcgc cgcactcagt atagccgccg caaaaatgat
     6601 ctttttcatt ctttgctcct ttattcgtcg ccgtactcct gttttatgtc agcaagcacc
     6661 tcttttatta tgtcgtatga ataccccaaa cgcacaaggg cgttttttac ctttatgtca
     6721 cccttttctc cgtcaagata tctgagatac tttcgctcca caagctcata cagccgctcg
     6781 tatgtaccct cgctgtattc ctccagagct tcctctatta taggcttcgt aagccctttc
     6841 tgtctcatct gctgataagc acggtaatat ccgtatttac gcacttccac ataagtcctc
     6901 gcaagcccct gagcataccg cctgtcgttg accgtcccaa gctccagcat cttatccatt
     6961 acagcgtcgc atatctcctc gctgtaattt ttctcaagct tcttgtaaag ctccacatag
     7021 gaatagtccc tgtagtcaag gagatacaaa gccctttcct tagcctttct gaactcgttg
     7081 tcaaaaacta tcttctccca agcagactca ggcatatcaa gccctgctct cagaccatag
     7141 gcagacacta tctcgtagtt gagaaaagcc gtttcgccct cctcaaactc tatcatgtat
     7201 gtgctgccct tgtattttga aacgcccgtt attttaagca tttatcagtc accaagctcg
     7261 cttatgtcaa caggagcgaa ctcctcgaag tcctcctcag gctttgccac aacagacttg
     7321 tcgcttgccg ccttgcgtat cttcttctca ggcttgtcag caaccatatc ttcatttgca
     7381 ctctgagccg cagcaccggc gttcgccttt tcttcaagct tcttggcctt tttatctttc
     7441 tgcgccgcaa gcacaagctc cgcctgatgt tcctttatct tggcttctat ctcgcccatg
     7501 atgtcaggat tgttcagcag gaactcctta gcgttgtcac gtccctgacc aagcttcata
     7561 tcgttgtagc tgaaccaagc tccgcccttc ttaacgatat caaggtctac cgccatgtca
     7621 agcacctcgc ccacacggga gatacccttg ccgtacatga tatcgaactc gcactccttg
     7681 aacggaggtg ctaccttgtt ctttaccacc ttgcactttg ttctgttacc aatgacctca
     7741 gagccgttct ttatctgctc tccacgtctg acctctattc ttacagaagc atagaacttc
     7801 aaagcacgtc cgcctggagt agtttcaggg tttccgtaca taacgcctat cttctcacgg
     7861 acctgattga taaatatagc cgttgtattg gactttgcga taacagatgt gagctttctc
     7921 atagcctgag acataagtct tgcaagctnn nnnnnnnnaa gctgaccaac gtgagtgtcg
     7981 cccatttcgc cgtctatctc agactttgtt accatagccg caacagagtc gataacgata
     8041 cagtcgatag cacctgaacg agccagagcc tcggctatct ccaaagcctg ctcaccactg
     8101 tcaggctgag aaacaagcat attgtctata tcaacgccaa gctgacctgc ataaacaggg
     8161 tcaagagcat gctcaacgtc gataaaagct acctctccgc ccattttctg tgtttctgca
     8221 ataacatgaa gtgcgaccgt tgtcttacct gatgactcag gtccgtatat ctcaataatt
     8281 cttcctctcg gcactcctcc aataccaagc gccatatcaa gggtcattga gcctgtcggt
     8341 atagcctcga tatccatagc cttagcctcg ccaagcttca tgatcgcacc cttgccgaac
     8401 tgcttttcaa tatatgctat cgctgtatca agagcctttt tcttttcctc tgcgtcagtt
     8461 atttttacta ccgtcttaac ggcttctttt ttcttcttgt ctatcgccat tttatatgac
     8521 ctccctatct tatcggtgtt ttatccgtat ctatatcata tcacactaac tgtccgtttg
     8581 tctgtacagc aaccgcaatt tatctatctg tacaaatttt cgtactattt ctcataaatg
     8641 cccgaaacac agcggtaaac accgcacaag tctttcctgc atctcacgtt cttaaagccg
     8701 tgctgtatca tcagccgcat tacctcgtcc tcctgaccga taccgatctc aaagagcata
     8761 acgccgccgc ttttaagctt tttcttccac ttgcgaacta tctcacgata gaagtccaag
     8821 ccgtcctcac cgccgaaaag agcttccgca ggctcaaaag tgacctcacg ctgtaaagcg
     8881 accatgtcct ctgttgtgag ataaggggga ttgcacacta taaggtcagc ctcaggcata
     8941 tcctccacaa gcttttcgtc tgtaacgtca cccatgacta tctcagcccc cgagccgttc
     9001 agacttatat tctcacgaag atactctgcc gccttttcag acttctccac acacactgcc
     9061 cgaccgcagt caagcttcct ttcaagggtg ataccaatgc accccgagcc tgaacaaagg
     9121 tctataacag tgatatcctt cttgcctgca aacatcttca aagccgtgtc aacaatagtt
     9181 tccgtgtcct gacgtggtat cagcacaccc tcgcccacct tgaaaggcag accataaaac
     9241 tcccactgtc caaggaggta ctgcaaagga tagccctcac agcgacgtgt gacaatgtcg
     9301 cttatacgct tttcctgctc aggagtgata tcatccccct cagccacatc ttcaaacacc
     9361 catgtgacct cgcaagcagc gttctcgcca agaccgctct cctcaagcag tcccacacat
     9421 ttatcaaaca gttctttcct taccatttgt catcttcctt attttcaggt atacaagcct
     9481 ttagtgccgc tatagcacat tctatctgac tttcatcagg ctcgcttgta gttatcctct
     9541 gtatccacag acccggtgca gagattatct tagtgaaaat attgtcatat tttcccgcaa
     9601 gccttataag ctcgtaagca atacccgtaa cgataggcag aagcgctatc tttataacaa
     9661 ctctcagaaa aatgcttgcc catgatactc tgaatattgt gtttacaaaa atgcttataa
     9721 acagcacaag gaaaataaag ctcgttccgc agcgtgggtg aaaacgtgtg tatttcttca
     9781 cattctcaac cgtcagctct tcttcatgct catagcaagc aatagtcttg tgctcagcac
     9841 cgtgatactc atatgtacgc cttatatcct tagtaagagc agtcaaagcc gtgtagccta
     9901 tgaatatagc tatcttcagt attccctcaa taagattctt tccaaaagcc gacaggtcaa
     9961 tgaacttgct tataaattta gacaccagcg acggcagata cataaacagc aatatcgcca
    10021 gacccacacn nnnnnnnnnc gccgtgatga tagggccgag cttgtcccca agcttatcgt
    10081 ccagccactt ttcaaacttg gtctgctcct cctcatcttc ctccgtcatc tgcttctcag
    10141 ccgaccttgt aaggcacttg tagccgtcta taagagaagt aaggaagctg aaaatgcccc
    10201 tcacaaaagg cgttttcctg taccaaggcg tatcttttcc gccttttatc gcccactcct
    10261 ccaggtctat ggtgccgtca ggcaggcggc aagccatagc cgccttgtgg atgccacgca
    10321 tcataacgcc ctcgataaca gcctgtccgc cgatctttga cttgaatttt tccttgcttt
    10381 gtgctttact gctcattttc tttcctttca aggcggtgtg tgcgtctact gcacgttgtc
    10441 gttgttttcg ataggctcag aagaaataag ctccacgtct acagttgcct gattgtcagg
    10501 ctttttcttc tgctcgatac aagggagcgt tatcatcaca gttgtaccta ctccctgctc
    10561 actgtttatc tccagcgtac cgccatgccg ctgaatgatc tcgtctgcca ccgcaagacc
    10621 tattcccgaa ccacgtcttg tgtggtttgc cttaaagaat ttcgtcttga cctttggcag
    10681 gtcatccttt gatattccca tacccgtgtc ggaaatggaa atacatacct cattgtcctc
    10741 ctcataagcc tccaccgata c
//
LOCUS       DAWEMR010000083        10737 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2584_length_10737_cov_8.838794, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000083 DAWEMR010000000
VERSION     DAWEMR010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10737)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2584_length_10737_cov_8.838794"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3088 a         2351 c         2092 g         3206 t
ORIGIN      
        1 tttatgtcta cagtttgtcc gccgcaaaat cgctgtcgct cggtgcttct tttactttat
       61 atttataatt ttttcttttt catttgaccc ttgttgcgtg gatatttctc aggtgtttca
      121 gatacttttc ttatgaggat aagcgttctg ccgtcgcctg ttggaagctc atagtcaaag
      181 acctcttcga cctttcctcc cagtatctta atagcatttt tagcctcttt cagctcgtca
      241 tttcctcctg agcctttcaa tgcaacgaaa tatccgccga cttttacaaa aggcaggcaa
      301 tactcgcata ggtcgctgag gtttgccact gctcttgctg tggcaatgtc aaattgttca
      361 cggcactcta tgcttctgcc aagctcctct gctctgccgt gtatgcaggt cgcaccaagg
      421 tcaagactgt ctgaaagctg tgcaagaaaa tttatcctct tattaaggct atccaaaagt
      481 gtaagcttta tatcgtcacg gacgattttc agtggacagg acggaaatcc tgcccctgtg
      541 ccgacatcta ttaccgttgc attttctttt acctcgaaaa gtgtgaaagg ataaacgctg
      601 tcaaaaaaat gctttattga aattccctca gggtcagtga tagcagtaag atttattttc
      661 tcgttccatt cacaaagcag atctgcataa gcttcaaagc gtgaataatc actgtcagat
      721 attgttatat cataattatc aaaaagcttt ataaaatcgt ccttagaaat aagctttgac
      781 atactattcc tccctatggt cattctttga tgagagccat accaaaagca cacccacgtc
      841 agcagggttt actccgctga ttcggctagc ctgaccaact gatacaggct ttattttatt
      901 gagcttttca attgcttcaa gtctaagact ttttatatca ttatagtcaa tatccgtagg
      961 caaagccttt ttctccagct ttcgcatagc ctccacctgt tcaagctgta tctttatata
     1021 gccgtcatat tttatctgca attctacctg ctcttttacc tctgctgaaa gctcaggtcg
     1081 ttccttatcg aaaggagcaa gaccgtcata tgacacctgt ggtctgcgga taagctgagc
     1141 aagttttatg ccgttgttaa gagggtcagt gcctacagat tcaagatagg catttatctc
     1201 aggatttggc gcaatattta tctttgaaac acgctttatc tcagcggcta tctgtgcatt
     1261 tttgtcaagc aaatgctgat atctttcgtc tgaaataagc cctatctcat gacctatagg
     1321 cgtaagcctt atgtcagcgt tatcttgtct tagcaaaagt ctgtattcgc ttcttgatgt
     1381 gagcattctg taagggtctg aacaaccctt tgtaacaagg tcgtcaataa gagtgcctat
     1441 gtatgatgac gctctgtcca gtataagctg ttttctgccg agaattttca gtgcggcatt
     1501 tatgcctgcg ataagacctt gtgcggcggc ttcttcatat cccgaagtgc cgttgaactg
     1561 acctgcgccg taaagaccgt caaaagcctt gaactccagc gtagcagaaa gctctgtagg
     1621 gtcgcagcaa tcatattcta tggcataggc atttcgcatt atctcaacgt gttcaagtcc
     1681 ttttatagtg tgcaaaaatt gaagctgaac gaactcaggc agtgatgacg acataccttg
     1741 cagataatat tcgtcagtgt caagacccat tggttcaata aaaagctgat gccttggctt
     1801 atccgaaaat ctcattatct tgtcctcgat agacggacag tatcttggac caatggcgtg
     1861 tatcctaccc gaatatatag gtgaaagatg tatgttatct cttattacct tgtgtgtttc
     1921 ttcatttgta taagccacat agcatttgac tttattttca agcttttcat gattatcaaa
     1981 agaaaacgga gttatatcat cgtcaccgca ctgaacttca agcttgtcaa aatctatgga
     2041 acgcttgtgt acacgggcag gagtgcctgt tttgaaacgt cttattgtca ttcctgcctt
     2101 tttaagacat tctgtcagac taagtgcagg caaagttgcg tcaggaccgc ttgaatatga
     2161 tactgcaccc acatggacag tgccgccaag gtaagttccc gttgagataa caacagcctt
     2221 gcagtcatac tcagtgccaa gctttgtacg cacaccacga acagttttgc cgtcctcctc
     2281 aaataaaata tcagttatct cagcctgctt cagataaagc ccgtcagtat cttcgatagt
     2341 ttttttcata agcttatgat attcaacacg gtctatctgc gccctcagac tatgaacggc
     2401 aggacctttt ccacggttga gtatcctgct ttgcagaaaa gttttatccg ccgccttgcc
     2461 catttctccg cccaaagcgt ctatctcacg aacaagatgt ccctttgcag ttcctcctat
     2521 agagggattg catggcatat tggcaacacc gtcaagtgtt atggtaaaaa gtgccgtctt
     2581 tagtccaagg cgtgccgccg ccaaagccgc ttcacagcct gcgtgacctg cacctaccac
     2641 aataatatca aaatattcct tttgcataat aaaaccacct gtctgaaaat aagaccatat
     2701 aaattacttt cctacgcaga attttgaaaa tacctcgtct accaccgctt ctgtagcttt
     2761 ttctcctgtc agctccaaaa gtgcatttgc gccattgtct atcatcacag taacagcgtc
     2821 caaagtctct cccatatcaa ggctttcaag agccatggca agatattttc ttgcacgttc
     2881 tgcacaggct atttgacgct catttgcaaa aatcgtgctg tcagcgtcat atccgccaat
     2941 gcctaaaagc ttctctgcgg ctttttgaag ttcttctctg ccctcgtcaa gcttcgcaga
     3001 aatctctatc acatagtctg aatatttttc aagctgagac ctttcaacaa cattttcaag
     3061 gtcagacttg ttgagaacta tgataacttt ctttccaagc tttttcacat attcaagaag
     3121 ctccaagtct tcctcgtcaa gctctcttga aatgtcgaaa acttctatta caagcatagc
     3181 gtttttaagc tttttctttg caatatcaac gcctattttt tcaacttcat catcagtttc
     3241 atgtatacct gcagtgtcgg aaagcttcaa aataagctcg ccaagctttg cactttcttc
     3301 gataacatct cgtgtagtgc ctgccacatt tgtgactatg gagcggtcat atcccaacag
     3361 catattcatg agggtcgatt ttccaacatt cggcttgcct gcgataacag tgtcaacacc
     3421 gtttttcagt atcataccgc tgtcatgatc tgctattatt ttatcaagcc ctgccaaagc
     3481 gtttttaagg ctctctctga gattatcctc agatatctca ggcaagtcct cctctggata
     3541 atccacccat gccgcaagct cacctagtat tgtgacaaac tttcttgaca tatcagaaat
     3601 cagcctgaaa agcctgccct cttttgtaag atttgcagaa ttaagtgcat attctccctg
     3661 agcggagata gtttccatta cgccctcagc ctgagtaagt gaaagctttc cgttgagaaa
     3721 agccctcttt gtgaactcgc ccctctgagc aagctctgca ccctgttcaa tgcaaagtct
     3781 gagaactttt tttgtaacat atattccgcc atggcatgat atctcgcaga cgttttcacc
     3841 ggtgtatgac ctaggtgcac ggaaaaccgt cagaacaccg tcatcaactt ctctgccgtt
     3901 tttgtcaact atttttccgt atgcacacga atagcctgcc atgttttcaa cacttttgca
     3961 tgacagcggt ttaaaaacct ttgcacctat gcttatggcg ttttctccgc ttattcttat
     4021 gactgatata ccgccctcag cgagtggtgt cgaaactgca catatatctt tcatttttac
     4081 ctctttttac tctgacagag cctttccctg ccatgaattt cttcttccca ttttcttgtc
     4141 tatctcgttc ataacgcaga attttttcaa actccacaaa ggtgcaacaa gatcgtcctt
     4201 tgcaccgtcg cctgtcacac gctgtattac tatctcagca ggcagaagct ccaactggtc
     4261 gcagacgata ttaacatact cgtccatatc caaagcccga aattctccac gctcatacat
     4321 ctgtgcaagc acagtgcctt ttaaaacgtg cagaagatgt atcttaatgc tttgtatatc
     4381 gagctgaccg agagttttag ccgtttcaag catcatatcc ctatcctcac cgggaagccc
     4441 gtcgatgata tgcgcgcaca cattaacatt gcgttttctc agcatttcat agcctttgag
     4501 aaaatccgca aaagtatgac agcggttcat tttcaaagcc gtttcgtcat gtatagtctg
     4561 caaaccaagc tctacagtaa gaaaagtctt ttccgcaagc tcaccaagga gatcggcttt
     4621 ttcttcgttg atgcaatctg cccttgttgc gatagaaagc cctgccgtat tttcaaatga
     4681 cagggctgta taaaacattt ttctcagcgt ttcaacgtca gcataggtgt ttgagcctgc
     4741 ctgaaaatac ggtatgcaca gacccttgct ccacttcttg ctcattttct cccttatctc
     4801 tctgtactga gtaaggatat cctcatgagg gtcgcctgca aaatctccgc tcatttttgc
     4861 cgagcagaac acacagcccc ccacaccttt tgtgccgtct ctgttcggac agccaaggtc
     4921 aatattaagc ggtatcttga aaaccttagt gccgaacttg tgccttagat aataattgta
     4981 ggtatgatat cttttattgt catcagaata gggaaaaggg ttatcaaatc tgctattcat
     5041 ctgacggttt ctcagtttgc tcggtggtag gttctgcagt ttcagtcggc tgagctgtgg
     5101 tctcagttgc ctttgttgtg gtcgtggtag tagtaggacc aggctgtact ataggctcag
     5161 tttcggtgac agtatagtaa ggcttgtcat tatttgtggt agtggaagcc gttgaagtcg
     5221 ttgtggtgct tttagtttcc tctggtctgt caatagcagt ggtagttgtt gtttttttct
     5281 tgtcctcttt tttctttgtg gtagttactt caccgtcctg cggttttgca gtggtggtga
     5341 taacattttc gtcaggatca tcactgtcat tattagtcgg atgatcgaag ctgttgtggt
     5401 tagaatcagt attgtcaggc gtttctttta tagggtcagt gaccttattg ccgataccat
     5461 aaataagaga gatacgctcc tcaacagctt tcatggcgtc aggaaaaggt atactattat
     5521 tataagagtc ctccaatgcc tgagtgtaat cccctgaaaa gaaagtcttg aatgttaaag
     5581 acagattgtt gtcctccaca atgtcgcttc ctgaaactga aataaagaat atgcaggcaa
     5641 atagcattat gaccgctatg gaaagaagat ttataaagct gtaatgctcc tccaaattcg
     5701 ttttggcaag ctcttcagcc gcttcctccg cagcctcgca ttcctccggt atctcatatt
     5761 catcgtcacc gtgaacgata ttgaatcgtg tagttttctt atccattagt attttcctct
     5821 ttttatgccg catattccgc cgcaagtgtt ataacgctca ctgcaaaaac aagcactgtc
     5881 ataatggtct ttgttatcat aactctgcca taagccgcct gagacttctg tgaaagcttc
     5941 ttaaaaatct tgcagaaagc cctttttgca ggtgggcaga caataaagaa cgatattact
     6001 ataagggtga tatttttcag cactgcactt tttacagaca cgctcaaggt gtatttcaca
     6061 tttactcctg caagtgcaaa gagccatttt ttgtagccat agaagctgtc aaaataaagt
     6121 ccgccaaaaa taacgagagc tgtcagcaaa agataaatat acttcacaag gtcaggcagc
     6181 tttgaaagcg gtctttcaag aaacagcttt tcaaggatac aaagcactgc cgcacaaagt
     6241 ccgacgataa tgaaattcac ctttgcctga taccatatag aaagtgccag accgcaaagt
     6301 atatagcaca caaaagtaaa gactttgttc atgttttttt tgcagaagaa aacctcgctg
     6361 aaaaagtcaa caacagttgt attgaagctt tttatcatac cagtgaaaag tccgtcagct
     6421 tcaatgtttg tgtagttgtc aggataaaca aagccgtttg taagaccaag accctttgcc
     6481 atatccgaaa gtcctgtgaa aagaaaataa tactgagcca agaatgacag cataccaagc
     6541 cagcaaccaa gggtagttat ctcttggccg ttaagtcctg caagctttat ctgttcaaaa
     6601 acgcctgcaa gaagtaccac cttgccaagt ccgaaaacta tctttgtaag accttcgttg
     6661 agctttgctc ctgtgatctc acgatgcctt atctgtggct caatatcact atatctcaca
     6721 agaggtccca caagcatgaa atgcagtgac accatataag tcaaaaggca gaaaatgttt
     6781 ttttctgctt tgcagttgcc cttgaaaacg tcatagacat aagaaattcc tctgagcata
     6841 taataaccca taccaagtgg aagtatggcg gcgtcaaaac tgagcttttt taccttatta
     6901 taaaggatat tctgaccgaa tattagcata agagatacat tcattataat atccagcgcc
     6961 aaaactatcc cgcaaagggc tttgttctca cggttttttg aaataataag accaaatatc
     7021 caatcgagga taactgacag gaaaataaga cagaacacaa aaggtctgcc ccaactgaaa
     7081 aacagcaggg aacaaactat cagaatgagg tttttatatt ctgcacttct gtcgaaaagc
     7141 gaaataatgg tcattatcgg gaacattccc agtataaata taagatcgga ataaaccaaa
     7201 ataaattgct ccttaatatt tttatatgta aaatattttt tatatttttt tgtttatgat
     7261 ttatacctat aattttatca caattcacta aatatagcaa gcaaaattat aaagaaaatc
     7321 taaatttttc agttttcttc aaaaagtatt tgcaattaat aaaaactgtg gtataattat
     7381 agtaaataaa tgtgcaattc tacttgggga aggagtggca gagttggctt caattttagt
     7441 tacaggagca agcggaatga taggcttgta ccttacatcc agcttactgc acaaaaagca
     7501 tcgtgtattc ggtatagata cacgcaaaaa tgagtttatt ggaactgatc caaactatac
     7561 ttttatccag tgtgatatta cagataagga cgctattaca aatgtaatcg atagtaacaa
     7621 gatcgacgtt gtcgtgcatc tggcaaattc tgtagataac gatatagatc catatgtaac
     7681 agacgctgag atgaaaaaga gcaagatatg tgataagttc atatatgcgg ctgcaaacaa
     7741 ggctgaggta aggagcttta tcctgctcag cacaacagca atatatggtg tacagaaagg
     7801 cagagaacca ataagggaaa ctgctcctga aaagggaaat tcaaactatg cagatcttaa
     7861 gatgacatct gagaagataa tgcagaagga atttaaaaag tctgaaacta tacctgttat
     7921 tgccagagta gctcctattt atgacgctga gtatacacaa aatctgagag acagagtatt
     7981 tgacgcaaag gaaaatgttg catacatatt caatgatgga gaatacggtt tttcattctg
     8041 ttgtgtattc aatcttatcg actttataaa tggtatcatc aatgtgccga caggaagata
     8101 tgagggaatc tacaatcttg ctgattcaag gctgataacg gcaaaagaga taattgatta
     8161 tgaaaaggaa catcaccgca taggtgcagt tatccagaaa tcacctggaa tgggactaag
     8221 catcaacaaa ggtaagatga aaactgatta tcgctatttt gacccatcta tcacattcaa
     8281 caactggaac atagacaaca caagggcaaa gagaatagca cctttcagat ggacactttc
     8341 aaacacaaaa taaactcaca aaggcagtcc cagggctgcc ttttttgtat tgttccatgt
     8401 ggaacatttg aaaaaacagg agaactaaga tatcatggaa agcaataggt tagttaaaat
     8461 attatgtgtt gtctgtgcgg caggctgttg tctttcctct tgcggaagca caggggaaga
     8521 aaagcccaaa aagctttcaa gcgtcagcac aggagtcacc acaactgtga ccacaaataa
     8581 aaagtctgcc gaaactatca cgggttcagt gactactata caaacagcag cgacttgtac
     8641 aaagatagtt tttgatggcg gctataccct ttttgcaaat gtgcagggtg gatatactct
     8701 tataaacagt tcaaatgaga tagtgaactc ttttgagata accgatgata acggtgataa
     8761 gcttgtactg acttgtgacg gaaatactgt cactgtcact aaaaacggaa aaaaggtaaa
     8821 ctcgttcagt tataaaggca caaggataat aatatcagcc aacgaggtct actatgcaaa
     8881 cacaaaggtg acatatgttg atgaaagctt cagcagcttt aacgtaactg acaaggtgat
     8941 tataagctgt atcgcagaga acaagtttga aattgtaaaa gacggcaaga cagtaaagaa
     9001 agctactgta aaagatgtga acggctcgaa gtatgtgctt gaagccatgg ataagggcat
     9061 ggagataaca aacactgata atgagcttat ggaatccata gtggtaggca ataactacat
     9121 aagcgtgtca ggggataagg tggtcataaa cggaaagacc atgcgaccac ctcacagtgc
     9181 agatataaaa gctgtgacta caactgttac tacaacaacg acagcggcta ctactaccac
     9241 aacgacttca aagaaaactg aaaagaaaac agaaacgcaa actgaaactg ttaccgagcc
     9301 tatgcaggaa gaacagcctt ctactcctca gcctgccgag cctactgaac ctgattcaag
     9361 cacgaatgtg tcaaatagaa atctttctgc tgaaacatct gaaatgcttg gctatgtaaa
     9421 tgaggtcaga aaacagtatg gtttaagaga gcttgaggga cttgagcttc tcgactctgc
     9481 ggcagatgtg cgagcaaacg agcttttgga aaattattct catgacagac ctgacggcaa
     9541 ttcatacatc acagctatag aagaaactga ccttcctgaa tggcgtgcgg cggctgaaaa
     9601 tatcgcatat ggtatgaaca gcatgaccac agtaaaagaa gcctttgacg catggatgaa
     9661 ttcaccgcct catagagaaa atatcctcaa ccctgatgtg cgttacatgg cgtgcgcaaa
     9721 agctacaaag tcagaaaacg gggaatacac ctattgggaa cagctttttt acagcgatat
     9781 ataaaaaaat tcggacagac ttttgagcct gtccaatttt ttttgttcca tgtggaacat
     9841 tttattctgc ctgcttttcc gatggtatct ttataatata ttcgatataa tcctcatggt
     9901 cgatacgttt tgcgtcagca ttcactcctg ccattctcat aacatctatc gccttattga
     9961 cactgttgaa aaacaatctt acatctttta acatagccgc tcttttgtga tagcttgcac
    10021 gcttttcatc atctttcaga agcttggcta tatatttttc caatgtgtca actgtccacc
    10081 cactttccgc tgctttgttc aacacagcaa gcctgcggtc attatctgtg atctttagca
    10141 aagcccttgc gtgacgttct gacatattgt tatcgagaat tattttgcgt tcctcagcgt
    10201 taagtttcag aatacgaagc ttgtttgcaa ctgtggattg agcaagtccc agacgaatag
    10261 ccgcagattc ctgagtcatg ccatattttg tgatgagctg agaaattgct tcggcttctt
    10321 caaaaaagtt caggtcacaa cgttggatat tctctataag tgcaatgacc tgcgaacgtt
    10381 cctctgtagc gttgataagt atgcatggca ctgaccttag tcctgcaagc tttgccgcac
    10441 gcagacgccg ttcgcctgac acaagctcaa aagtaccatt cactctccgt actgtgagag
    10501 gctgaatgat accgtcctga gaaatgcttt tcgcaaggct tgtcagctcg gcaggtgaga
    10561 aatatcgtct tggctgaaca gggttcggcc ttatctctcc tatggaaatg tcgattattt
    10621 tgcctaacgt ctgatagggt gaagcccttt gtgattcgtc agtttcttcg ctgaaagctt
    10681 ttgttaatgt ttttggaaaa atgctcatat tttatacctc cgtttcgttt taaaata
//
LOCUS       DAWEMR010000084        10731 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2587_length_10731_cov_11.453634,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000084 DAWEMR010000000
VERSION     DAWEMR010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2587_length_10731_cov_11.453634"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2888 a         2262 c         2410 g         3171 t
ORIGIN      
        1 ttgatggcga gggttttgtg cctcataagg cagaggtagc tgagagaatc tctatgctaa
       61 aatgctgtag tttgttgggc atttgtcttg tggtaatgct tgctattgat tttcttaact
      121 attttatagt gaataaatac gcgccggata caactggcac atttgtgtat ttttctcaaa
      181 cagaaggcgg atatggcaga tatgatgttg gaatgacttt tattttaggt ctgttgtttg
      241 ccgcaaagtt tgttgtgcct ggtcttattt ttaagatcgt tactaagatc cctaataaag
      301 tcgtttttgc aaattcaaag ggctacgaaa aaatgcgtgg aactgcagtg gtaattatga
      361 tggtcataat cgttgtgggc agagttggac aatatatcct tagtctgctg ttttcagctg
      421 tgcatttaga cgggatatat tctatttttg tattctctga agatcctgtt gtagctcttg
      481 tttcttttgc tttctttgcg atcattgttc ctataatgca agagatagta tttcgaggaa
      541 tttttttgca gacttttcgg caatttggcg acaccttcgc tattatgatc actgctgttg
      601 tgaacggttt atgctactat gatattacat atctgccttt tgttgtatgc tgtactgcgg
      661 ttcttggact ttttactata cgaacaggct cggtttttac tgcaatttca atgagtattt
      721 gcgcccacat cacgaatttt gtattaagtt acattgttct ctatgatctt ccttatgcca
      781 aaatagcaga agtcataatc tgtacagtga tcgttggtgt atcacttgtg atgtactcta
      841 agcttacaag ctttggtgac tggacattca acattgaaaa tgataattct tttatgacaa
      901 tgtcaaagcg agttaaatct ttcattgcaa ctaactcgat cgcagtgtgg atagctgcaa
      961 ttcttgtgac catgtttgtg tgtgcgagga taatatgacg agagatgagc tttacatgaa
     1021 aaaagcgttg gagcttgcgg aaattgccgc agagcagggg gaagtacctg tgggtgcgat
     1081 agttgtgaag cgttccagtg gtgaaataat cggcagggga ttcaatcgtc gtgagtatgg
     1141 gcattcccct ttgactcatg ccgagcttgt ggctattgag gaagcaagtc gcaatcttgg
     1201 cggctggcgg cttatgggtt gtgagctttt tgttactctc gagccttgtc ctatgtgtac
     1261 gggtgcgata ataaattccc gtatcgagcg tgtggttttt ggtgcgtatg acaaaaaggc
     1321 tggttcttgc gggtcggtgg taaatctttt tgagctacct tataatcaca gacctctttg
     1381 cacaggcggt atactgcaag agcggtgtgc agatacactg acggaatttt tcaaaaatct
     1441 tagacgtaaa aaaaacctct ccgaatagtt cggggaggtt ttccttatat ttcgtttcga
     1501 ttttccaaag ccttgtaaag cgtcacttca tcagcatatt caagcactcc gccaacgggt
     1561 agaccgaacg ccaaacgtgt gacctttaca ccaaggggtt tcagcagcct tgaaatgtaa
     1621 attgccgtag cttcaccatt aactgtagga ttagtagcca taatgacttc tttaacattc
     1681 tcaggctcta tcctcgcaag cagttctttt attttaagct tgtcagggga aatgtcgtcc
     1741 ataggggaga gaagtccatg caaaacatgg taaacgcctt tgtactcctt agtacgctcg
     1801 aaagctgcta tatcctttgg gtcttctact acgcagaccg ttgtgtggtc acgtcttggg
     1861 tcggcacaga tagggcactt tggcttgtca gtaaagttct ggcaaacact gcaaagatga
     1921 acgcttttgt gggcattagt gatagccgct gcaaaagccg atgcgtcctc gtctgtcatg
     1981 gacatgacat agtaggcaag gcgctgtgcg gacttcatgc ctataccagg gagctttgca
     2041 aattgctctg ccagaagtac caaaggcagt gcgtttgact cagccattaa aacagacctg
     2101 ggaatgatac tccgcctgtg agtttctgca tttcagtgtc gcttacttca tcgaggttag
     2161 caacagcctg attgaaagct gccataacga ggtcctgaag tgtttcaacg tcctctggat
     2221 caactacctc aggcttgatg tcaagcttaa ggacgtttct cttgccgttg atagttacct
     2281 caacaactcc gccgcctgct gtacctgtaa actctctctg ctcaaggtct gcctgaagag
     2341 ctgtgatctc gtcctgcatt ttttgagcct gcttgagcat ctgattcatg ttattggaag
     2401 gacccttgcc cattccctgt ggaagtcttg ctttcatttt aaaaactcct tatattcaaa
     2461 attatttttt tatatcaact tcaatatcaa gttttttcgc tttgtcaagc aactgattta
     2521 taggattttc cgtatcctca ggtgcgacat ttttggctga tttgactttg atagtgaagt
     2581 tttgtccgaa attatttttc agtatctcat taagcaccgt agcatcgccg cttgctttga
     2641 actttttgag ataaaagtca ttgtgtacta tcataagcaa cacattctga cacatataag
     2701 cctttgaatt gactaagaaa cagcctatgg caggatttgt ctgcttgacc tgctccacaa
     2761 tgttgtccca agtctgcaca agcttgaagt cgcttgcttt cattttcttg aaatccgggt
     2821 caaaaggctt ttgtggggga gcttgttcct gctgaactgg agtggcgatc tgcggttgag
     2881 tctgcaaaac ggcaccactt tttagtgcat tctcaagctg agttattcta ttaagaagca
     2941 ctgtgatctc agcattgcct gatgcagccg gcacagttgc acctgctttt tgagcagctc
     3001 cgcacagctt tatgatacac atttcaagct cgatacgctt tgatgtgctt tttgcaaaac
     3061 gctcgctgca ctcctgcaaa gtatttatat ccccgagtat ctcgtcaagc ttcatgcgtt
     3121 ctgcaagggc tttaagtctt tcatactcgt caggcagaca agttattaga tccttgttgt
     3181 tgtcgatagt tttgattatc atgacatttc tcatctgagc gagcagttca cctgccagga
     3241 ctttcagatc ttttgacata tcataaagct tgtcaacgac cctgagtgcg cctgcggcgt
     3301 ccttttcggc tatcttttcg agaatatcaa aaagataatc acgtcctgca atgccggaag
     3361 ctgaggacac aacgtcaagg gtgatgtcat tttcataggc aacgcactgg tctaaaagag
     3421 aaagtgcgtc acgcatacca ccgtcagaaa gccttgctat catgcctgcg gcatcttcgt
     3481 ggagggtaaa gccctcctga gaagcaatat acataagcct tgaaacgata tcctcgtttc
     3541 ttatcctgtg aaagtcgaaa tgctggcatc tcgacacgat agtagcagga actttatgta
     3601 tctccgtagt ggcaagaata aacttgacat gcggaggagg ttcttccata gttttcagca
     3661 gggcattgaa tgcgccggcg gaaagcatat gcacctcgtc tatgatatat accttatatt
     3721 tacatctttc aggagtatag acaacgcctt cacgcagttc tctgatatcg tctactttag
     3781 aatttgaagc agcgtcaatc tcgataatat cggaaagagt gccgttgtca gcgtctttgc
     3841 atatctcaca ttcaaggcat ggctcaccgt catgtgggtc aaggcaattg atagcctttg
     3901 caaaaattct tgcgcaggtg gtctttcccg tacctctcga gcccgtgaaa agataggcat
     3961 gagcggtctt gccgcttttt atctgatttt tgagagtagt tgtaatatgc ggttgtgaaa
     4021 caacatcgtc aaatgacatt ggacgccatt ttctgtacaa tgcctgatac aaaactctca
     4081 ctccaatcaa gaaaataaaa aaatcaatcc gacatatggg cgtgcgggcg gaactgtgac
     4141 acacgaaaca aaccgcttaa tgctgctcgg ttccccgcct gacatggttc acagcgtttc
     4201 attgcacagg acccgcacac ctatatctcg gattgatgag gaacaataac tgaatcatat
     4261 caatctaact tttgttacct tactattata acatacttcc aatgaaattt caagtgtttt
     4321 tgaaaaaaag ttataaaaaa ggcaaagaac atcaacgccg ctgaacaaag ctggacagcg
     4381 gcgaatatta tgtgcttgta aaattatatc aggtggcaat gatgaaattc agcttgccga
     4441 acggatagtt cactgggata cagaatgcag tgcctgacag ggcaagctca ccattttcca
     4501 aaaattcctg cggtgtgagt gtaagctcaa cgccatgctc gtttgactca tttacaacga
     4561 acagaccatc gtttacgttc agaaattcgc ctgctgtagc gtttacaaag tcatcgacct
     4621 ccgtgaagct ctctttggca aacctctcag cgaaagccat atatgtcttg gaatctgtgg
     4681 caatgcaagc ctcagcgtta tattttccat ttattttttg gataacgata tgttctgctg
     4741 caaaattctt tataactgac gcttccatag gagtgaaatc gtcaccgata aatctgataa
     4801 gatttttgaa aagcaaagta acataatcag tacagatacg agcattttca tcattgtcaa
     4861 aatgatagaa ctcattgata aggttatgta gagtttctgt ttgattgtct gaaatatcgt
     4921 cgcttatctg attttcagcc ttatagtctt taagtgcctt ttcaaactca gcattggtaa
     4981 gagcaccgtt attaacaagg gtctgaccca ggagcaggta atctgtaggc tgctgagcga
     5041 gaagcttttc aacgtcgtca gcggtaacat agccaagctc cacggcaagg tcgccaaaac
     5101 gtttatcgac actctgctga gtgatattga cgtgctccac ctgctcggct gtcataagac
     5161 cagcgtttat agcaagaacg ccaaggcgaa gtcttgtatg ctttttagcc tcgaaagcct
     5221 gagtaagatc ctcagcggta caaagcttat ggttaaagag gtagcctcca aaaaactgcg
     5281 caaacattta cagtaccccc tttgcaacgt tctcaagaac tttaagagcg tcgttatcag
     5341 ctataggttt ctgcaaaaag tccacagctc ccgacttaag agcgtctttc aaatggctct
     5401 gagtaccaac agaagaagcc attatcacct tagcgtcagg gtcttctgct attatctgtt
     5461 tgagggcttc aatacctgtt acctttggca tgatgatatc catgaaaacc acgtcaggct
     5521 taaactccct atatttgctg acagcctctt caccgtcggc tgcttcataa acggctttta
     5581 ctccaaatcc agaaatagac atactgagct ttttgcgagc gaacatagaa tcgtcgcata
     5641 taagcactgt catttcattt atattcacga tagatccctc ccgattattt tgcggcagca
     5701 gttccgtaat tgccgatgac ggagagaata atttctctac tgccttgttt ttaattatat
     5761 cataaaattc tccaaaagtc aagagtcgtc aaatatgttt tatgttgaaa atatacaaac
     5821 gagtaaatgc agaatttact tgcagtttgc ttgacaaaat gaaaaaagta tgctataatc
     5881 atagtgttat gtgccggtgt gatggaattg gcagacgtgt cggactcaaa atccgatggt
     5941 agcgataccg taccggttcg accccggtca ccggcaccat gaaaacgggt tcttgtatca
     6001 agagcctgtt tttttattag tgtcctcatt cttttctgtc tctgcttcac caccaagagc
     6061 attggcgatg acctcggctt gacagtgctt tcagacgaac ggaaacgccc ctcagagccg
     6121 tccgagcctg cggagcggaa aactaaccgt ctgaaaagaa aagacctgag aagtgcttgt
     6181 gtgagcaaat ctcagttctt tgtgagttat tcagttccaa aagggtatgc tgtcattgtg
     6241 gcgcgtgcca taatgatgat atggacttgt gtggaacggt ttgcaggtgt tccaatagac
     6301 tacgggcaag cggcacacat caatattgtg ccttaacgaa atcttaacac cgtaaccgta
     6361 atcttaacag cactttaacc gctcatgtga tataataaag ctataatttg aacgagaggt
     6421 gtacgatgtt acataaaaat agagtaaaaa acagaactca tatgacttca tttcgggtca
     6481 tcatcatcag tttcttttgc ctaattctgg taggttcgtt gcttctgatg ctgcctatct
     6541 catcaaggca gcggtgtgtg acttcattcc ctgatgcaat gttcacagcc gtttcagcta
     6601 cctgtgtgac tggacttgtt gtaaaggata ctgctacata ctggtctctg ttcgggcagg
     6661 cgatcattct gatgctaatt cagatcggag gtatgggcgt tatcaccgtc gggcttgcga
     6721 tcattagagc atcagggcgg aaaatcggtc tgtggcagcg cagtacgatg caggaatcca
     6781 tctctgcacc gcaggtaggc ggtattgtga agctgacagg ctttatcctt aaaacatcac
     6841 ttatcataga gatgatcggt gcggctttgc ttgcacccgt tttctgcaat gatcttggta
     6901 tcgctaaggg actgtggtat tccttgtttc acagcatatc ggctttttgc aatgcaggct
     6961 ttgatctgtt cggcataaaa gaaccgttct catcgctgac cttttaccac agcaacatct
     7021 atctcaatat catcattatg ctgctaatta ttacgggagg catcggcttt ctcgtctggg
     7081 gcgatatcgg aacacacaag cacaggatca ggcgctactc tctgcaaagc aaggtcgtac
     7141 tgatgacatc tgctttcttg atcgttctgc ctgcgctgta tttcttcttt tttgaatatg
     7201 accatgggac tatccatgac cggacgatcc attcactgtt tcagtctgtc acaacacgaa
     7261 ccgcaggctt taacacaact gatctcgctg ctatggatga atcaggtaca gccattatgg
     7321 tcattcttat gcttataggc ggttctcccg gttccaccgc aggcggtatg aaaacagcga
     7381 cttttgctgt tctgttcctt tctgcgatca cagtgtttag ccgcagaaat gatgtgcagt
     7441 gcttcaaaag aagaatatca gacgaaactg tccgcagtgc aggcgctgtt ttgtttatgt
     7501 atcttgtatt gttctttaca agcggtgtca ttatcagcag agtggaagat ctaccgctgc
     7561 tgacctgtct gtttgaaaca agctcagcta tcggtactgt agggctgtca ctaggcatta
     7621 caagcggtct gtctgctgta tctcgtgtga tactgatgat acttatgttt ttcggaagag
     7681 tcggcggact gacactcatt tatgcagctc tgccgtcggc ggacagtcag aattcacgct
     7741 taccgcttga aaaaatatct gtagggtaag gagtcttata tatgaaaaca gttttaatta
     7801 tcggagtagg tcaattcggc agtcatattg ccagacgcat ggaggagctt cggtgtgagg
     7861 tcatggcagt agatgaaaat gagaagcata tcaacgatat cctgccttat gtgaccaacg
     7921 caaggatcgg tgacagcacc aatgaggaat ttctccgttc gcttggtgta ggaaactatg
     7981 atgtttgtat tgttgccctc ggcagccggt ttcagtcctc tctggaaaca acgtctttgc
     8041 tcaaggagct gggggcaaag aaagtcatat cccgtgcgac aaatgatgtg caaatgaaat
     8101 tcctgcttaa aaacggagcc gatgaggttg tctatcctga gaagcagatg gcacttcgca
     8161 ttgctacaaa atatgcatca gacagcatac tcgactttat tcatctggat aacaattact
     8221 cgatctacga actgaaagtg ccgaaggact ggttcggaaa gtcgctttca cagatcgata
     8281 tacgaaagaa attcaagatc aatattctga cgatcaaacg aggagaagag gtatttatcc
     8341 cttcaccgga cactgtaatt gaaactgatg acatcgcatt tgtcataggt gaggttcggg
     8401 atatccagaa gtgtttcaga atctaggaga tgatcgtgtg agagatgttc tgcttgcact
     8461 gtctgtagga gcaatgtttc tctacggttt ttatattatg aaaaaagtag atggattcat
     8521 cgaggagata caaaagagca gacgtggaca cgatctgtca gaagatgatg ttgacgaaca
     8581 caaaagtgat atgttataat aacaagggtg atccttatga ctgctaattc tataaaaagt
     8641 tctaaagcat tttctgtgtt caaagactcc gctatatgta ttgtggtgct gacagccgca
     8701 acacttgccg gatacgcatt caaagccttt caacttgccg atgcggacat tatcatgctg
     8761 tatattattg ctgtgcttgt gatatccatt tttacatcaa agatgtattt ctgcctgata
     8821 tcatcattag taggagtgat gcttttcaac tgcttcttca cctatcctga gttttctctt
     8881 tcagttcacg atgcaggcta tcttgtcaca tttgtgacga tgtttattac agcgttcatt
     8941 gcaggaacgc ttgccaataa gctgaaacgc aatatcctga tcgctgagca gaatgcccgt
     9001 gaaaaggagg aggcggcact tctggcgcag aatgaacagc ttagagctga tatgctccgt
     9061 tccatatccc atgatctcag aacaccgctg acctccatat caggcaatgc cagtacgctc
     9121 atttccggcg gcgatacgct tgatgaatca gcacgtcagc agatatacac ggatatttat
     9181 tccgaatcaa tgtggcttat tgaaatggtg gaaaatcttt tatacgctac acgaattgag
     9241 gacggcagaa tgcagctcaa tatctcggta gagatacttg atgacattgt tcaggaggct
     9301 gtcaaacaca ccaaacgcac tcacccaaag cgaaatatca tcgttgatat gtatgacgag
     9361 ataatccccg ttatggcaga tgcgaacctt atcgtacagg tcatagtcaa tcttatggat
     9421 aatgctgtta agtacagtga tgaggattct gatgttaccg tcagtgtgcg ccgagaaaat
     9481 gcgcatactg ttgtgatatc cgtgtccgat cacggctctg gtatctccga tgaagaaaaa
     9541 gaaaaggtat ttgatatgtt ctatacgggc ggcagccgtt cctcagacag cagaagaagt
     9601 ctggggctgg ggcttgcact gtgcaggtca attattactt cacacggcgg tactatatct
     9661 gtcagcgaca acataccaaa cggtacagtt gtcagcttta ctctgccaat aggagaggtc
     9721 gatttaaatg aatgaatatc ttgttctggt ggtggaggac gacaagccta tcagaaatct
     9781 tattacaact actcttaaaa tgaacgacta tcgctttatt acagctgtca ggggcaacga
     9841 agcgatcatg ctcagcgctt cgcataagcc tgatattatc atacttgacc ttggactgcc
     9901 tgatattgac ggcgttgagg tgatagagca tatacgcact tggagcgatg ttcctatcat
     9961 catagtcagt gcaaggagcg aggaaaggga caagataaca gcccttgaca aaggtgcaga
    10021 tgattacctg acaaagcctt tctctgttga cgaacttctc gcaaggctca gggtgataca
    10081 aaggcggctt atgaagtccg aaaacatttc ggtcacggag tttgtcaatg gcaggctacg
    10141 gatagactat gtttcgggct gtgttcacct tgatgatgaa gaacttcacc tgacaccgat
    10201 agaatacaag ctgctttgcc tgcttgccaa gaatgtcggc aaggtgctga cgcataagta
    10261 cattatccaa agtgtatggg gaactcctgc ggataacagc gaagcctccc tgcgtgtatt
    10321 tatggctacc ctgcgaaaaa agctgagtga cagcagtcag gcgctgatac aaacacatat
    10381 cggtattggg tatcgtatga tgaaattata gttttaggga gcagtacaaa gcagacactt
    10441 tatttgatga tctgtatcgc cttcaatgtc tgcaaaaaca attgaactga ttttttctaa
    10501 aggaaaaaag ggcaatgcct tccactcacg gaggacattg cccttttcat tatgctgtga
    10561 ttatatcagt cctgaatctc ctacatcata gttgcgatcc tgtataataa aacttgacac
    10621 atacaaaaca atcaaagtat aataaaaaca agggagtgtg tcatcaatgg gaacatgaaa
    10681 acaatatgat gaagagttta aaaagcaggc aataaagctc gcaaaggaga t
//
LOCUS       DAWEMR010000085        10666 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2611_length_10666_cov_10.790595,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000085 DAWEMR010000000
VERSION     DAWEMR010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10666)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2611_length_10666_cov_10.790595"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1413..1422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6814..6823
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2933 a         2370 c         2342 g         3001 t
ORIGIN      
        1 taatcatgta tgagcagatc atggataccg attatctcag gaaagctcat tgcagtgtca
       61 acgatatcct gaacgaactc tttgtcaggc tttgagccga gaagcggcga aacactgtcc
      121 ttgcaggtca taaagcctga gcggaagata aagagagcaa cgagaacgcc catatagccg
      181 tctatgttta tgcctgtgaa cgcagagaat accattgcaa gcataacagc tcctgttgcg
      241 gcgcaatcgg aaagactgtc agcggcagag gcgaaaagcg aggtagagtt tatctttttt
      301 ccaagctttc tatagaaata ccacatccaa agcttcacag ccacagagca ggcaagtatc
      361 acaaaggtca ttaccgagaa agcaagctta tcgtgggcaa agatctttga aaaagaagat
      421 tttccaagct caatgctcat catcattata ataaacgaaa tgacaagacc tgagatatac
      481 tccattctgc catgaccgaa agggtggtct tcgtctgcgg ctttggctgc cattttaaat
      541 ccaaccagtg acattataga cgaacctgca tcagaaaggt tgttaaaagc gtccgccata
      601 acggacacag cccctgacac aaatcctgca ataagcttac cgaggaaaag catgatatta
      661 aggactatac cagtacatga gcccaggtag ccatattttt tacggacgtt tttatctttc
      721 acgttatctc tgtcttttat aaataatctt ataagaagtg ctgacatatg aatatcccct
      781 tttgtttacc gaagtaaact tataagttta tctactatta taacacataa tattacaaaa
      841 tcaagtgcaa atgcttattt atatggatat tcgccgcagc aggcaatatg cagccactta
      901 tttctcattt ccaaccacat aataaaattt tcttttcact attgaaaaaa tttgtgcagt
      961 gtgctataat agtcattagc attaatcgtt tacatacgca aaaagaaaag aggttatata
     1021 aaaaatgaga aaattcatat cgagagttgc cgctctgctg ctggcaggag ttatgacagc
     1081 ggctgtggca ggctgcggaa aaaactccga cagcgaggca aaagacacag caaagaagtg
     1141 gacagagctt gaccagacac agataacaga agccatgggt cttggctgga acttaggcaa
     1201 tcagcttgaa gcttccagcg gcggacttcc cagcgaaact tgctggggca accctgagat
     1261 aaccaaagag cttatcgaca ctgtcaaggc gcagggcttc aagacagtaa gaatacctgt
     1321 ttcttatctt gacatgatag gagacggtcc tgactacaag atagatactg actggctgga
     1381 cagagttcag gaggtcgttg actatgttgt tgnnnnnnnn nncaacgata tgttcgctat
     1441 agttaatatg catggtgacg gatattacac tgttgaccac tcttggttgc tttgtgctga
     1501 agacgatgac aagcagaccg agataaagga caagtacggc aaggtttgga ctcagatagc
     1561 agaccgtttc aaggactatg accagcacct tatcttcgag tccatgaatg aggaattcaa
     1621 caacgattac ggcaagcctg acgaaaaagc atatgataac atcaacgcat acaatcagat
     1681 atttgttgac tcagtgagag caacaggctc gaacaatgaa aaacgctggc tgcttctccc
     1741 tggttggaac acaaatatcg aatatacagc aggagatgat tacaacttca agataccgac
     1801 cgacaacggc tgcaaggctg acggcaagcg tatcatgata tcagttcact attatgaccc
     1861 attcaatttc actattgacg agaacaagac cgcaaaaact cagtggggca aatatgcggt
     1921 aaagaattat gacaactggg gtcaggagga ttatgttgac agccaaatgg cacttcttaa
     1981 cgagaagttt gtaagtcaag gttatcctgt tgttatcggc gaatttggcg cacaggacaa
     2041 gacagaaaag tttgcagact acaatgagtt cagacgctac tgggctgaat atcttatcaa
     2101 ggcggcaaag aaaaacggcg ttgtttgtgt atactgggac aacggctaca acggtaacaa
     2161 gggctttggc atcataaaca gatttgacta cactatcaca cagcctgacc ttatcgcagg
     2221 catgatgaga gccataaact ccacagacga ttatgagatc ccgtcaccgg caggctacac
     2281 agaggtcaga aagagcgcaa aggcgtcata agtttttcac acacatatat gctgatacat
     2341 ttgcaggggc gaacagcgtt cacccgtttg atttgcatga tgaaataata aaagcagtac
     2401 ctttttgggt actgcttttt gtttgtgtct tacgaaacga gagtgggcga attgcgggcg
     2461 aacgctgttc gcccctactt tttgctattc tattacaagt tcctcttttg tggaatccgt
     2521 tccctcgata tggaaagaat tgagcacagg ctcatctctg ttcataagtg agagtatcat
     2581 atagcttgct gtcttgtcat aagcaagcct tttatcctca tcagaaggac gagaaggtga
     2641 ctcagggtgt gagtgccagt tgccaagagg aacaagtccc tctgctctca tgtccttgat
     2701 agcagcaagc tgctccttag ggtcaagaga aaaatgctcg tttgagtggt ctatgttcgt
     2761 gagaagatag acctttttta ttatcttgtc actgccctct tttactccgc cgataagacc
     2821 gcaggcttcg tttggaagct cgctttcagc gtggtgcagt atcttttcat aatcgctttt
     2881 gcttagtttt atcataaatg tacaccgtca cattctgcct gttcatagtc gataagctct
     2941 gtgatagtag ggtgctcgcc acatactgca cagccctttg tatcctgagg gagctttacc
     3001 tttctgaact ccattttaag tgcgtcataa gtaagcagat agcctgtgag aagctctcca
     3061 acgccaagga tatacttgat agcttccatt gcctgcaggc ttcctataac gccacccatt
     3121 gcaccgataa cgcctgcctg cttgcaagta ggaacagcgt ccttcggagg tgggttcttg
     3181 aacacgcatc tatagcatgg tccctgacct ggaacatagg tcatgagctg acccttgaat
     3241 ctgatgatac cggcgtgtga aaatggcttt ttagccatta cgcaggcgtc attaatgagg
     3301 aactttgcag ggaaattatc agtaccgtcg ataacgaaat catagtcctt gattatatcc
     3361 atgatgttct cgctgtcgat aaaggtgcgg tatgtattca ccttaacgtc aggattgata
     3421 gcctccattg tttcctttgc ggaaagaacc tttgccttgc caacgtcctg agttgtgtgg
     3481 attatctgtc tttgcagatt tgagagatct acctcgtccg cgtctgcaat gccgatagtg
     3541 ccaacgcctg ctgcggcaag atacatagcg gctggtgcgc caagtccgcc tgcgcctatg
     3601 ataagtacct ttgcgttaag aagctttttc tgtccctttg cgccaacttc tttaagaatg
     3661 atgtggcgtg agtaacgctc aagctgttcg tctgtaaatg ccattactgt ccgcctccca
     3721 tgaaataaag aaattctaca acgtcgccgt ctttaagaac tgtgctttca aaagcaccgc
     3781 tctctacaaa ttcgtcattg atagttactg taacatactg cggtgtttca acgttctcgt
     3841 cgataacgag ctgtgccact gtaagcccct ctgcaacgtc ctttttattg cctgctactg
     3901 ttatagtcat atccatttct ccttaatcta tagaatttat tgtttcggaa agctgtgctt
     3961 tctttacagc tcccgtaagt cttgcttttt ctgctccgtc cttgaagaac acaagtgtcg
     4021 gaacgcttgt gacgttatat ttctgagcgg tttccccgtc aaagttgata ttgagcttca
     4081 caagctttat gccctcaggg tcttcctcct cagtttccgc aagaagcggt gacattcgct
     4141 tgcatggaac gcaggaatct gaatagaaat ctgcgataac aagctttcct gcctcgttta
     4201 tctcgctctc gaaagtatcc ttatttgctc ttactgacat taatctccca ccttttgtac
     4261 gataaggtca taagtgccgt cctcattgtc aatgagcttc aatatcttgt ggccctcttc
     4321 cttaatgctt cttggaacgt tctgaacagg ctcgccgtca ttcattttaa tggaaagaat
     4381 ctgtccgtcg tcaagctcct caagagctac ctttgcctta acgaatgttg tcgggcaaac
     4441 cactgtggtg atatccactg tatcgtctat tttaaaatca gccattgtta taaacctcca
     4501 gtatagtatt tgtaaatttc tctctgccca ctctgtcgat agtgaactta aatctttcgc
     4561 cagcttttgc gttatcgtca aagaactgta tagcggtatc gcaaatcttc ataagagttt
     4621 ccttatcctc gataaacgga ataacagttt cgcccttgtt gatatggttg ccgaaaagtc
     4681 cgccgaatga aactatatag ccgtggatag tatcccacgc ttctgtagga caagccttaa
     4741 aacagcgtcc gcagaaattg catttctctt tgtcaaggac tatctttccg tcctccattg
     4801 taagcgcacc ctgacggcaa gcctttacgc acacgccgca gccaatacaa tcagcttcac
     4861 gccactcgac ctttatgcca cccttgatac caacgtcatt ttcttccgct ttaaggcagt
     4921 tattctgaca gcctgtgatg ccgaacttga acttgtgagg aagccccctt gcgaaatatc
     4981 tttcgtcaag ctcctttgca agagcgtatg tatctataca gccgcttgga cagaccgcag
     5041 ctccctgaca ggctgtgata gttcttactc ttggtccgca cacgcccggc tctgcgtcgc
     5101 cctttgcaag agccgctttt acctcgtcaa tatcgtcaag ctttataaaa ggtatctcaa
     5161 cgctctgacg tgacgtaaga tgaacatagc cctcgccgta tttatcagac acctctgcaa
     5221 tagttgcaag ctgttttgcc gtaaggtttc cgcccacaac tctaagtcta agtgaaaaat
     5281 tattcttctg cttctggcgc atgaaaccgc cttttttaag tgttgcgtag tcaactgcca
     5341 tagtaaattc ctcctaaagt ttttctatgg actgtttaaa gtcctcgatc agatccgatg
     5401 cgacctctat tccgacgctt atcctgattg tgtcgccgaa cacgcctgcg tttatcttct
     5461 gctcctcact gccgtgggcg tagatcgtac ttgcagggtg tataacgagc gttcttgtat
     5521 ctccgatatt agttgcattt gttgcaagtt tcaagccgtt tataagcttg aaggctcttt
     5581 ccttcgagcc tgctcttatg gtgagtatag caccgccctt gccgccaagt tcttcctgac
     5641 aaaggtcata gaacgggctg gctttcagtg caggatagtt cacttcaatg ccctcacagc
     5701 tttcaaagaa ctccgcaagc ttcaaagcgt tatggcaaca tcgctccatt ctaagaccca
     5761 gtgtttcaag tcccacgctg ttcataaatg cgttcatcgg agcaagacag ccgcccacgt
     5821 ttctccatat gccgtttctc agctttgcga tataagcaaa cttcccgaat ttcttatatt
     5881 ctgccaaccc cttgaatttg tcataatccc atttgaagtt tccgctgtcg attataacgc
     5941 cgcttatgga atttcctccg ccgtttatat actttgacgt ggaatgtacc acgatatcag
     6001 caccgtagtc aaatggattt ataagataag gtgtagccgt tgtactgtca agaataaaca
     6061 ctgcaccgtt ttcatgggca aggtcagcca ccgactttat atcaactatt tcaagattag
     6121 ggtttccgat aagctcggca aagaccgctt tggtcttact atttataata ggggctattt
     6181 cctcttttgt aacgtgcttt acgaacttgg tcttgatacc gaatgccgca aggtcgccga
     6241 aaaggtcgat agtgccgccg aaaagacctg cgccgcatat tatctcgtcc cctgcttcaa
     6301 gaatgttcag aagcgacatt gtaacagccg ccatgcctga tgaacacgcc actgcgccaa
     6361 cacctttttc aagtgtattc attcggttct caaaagccgc cactgtcggg ttgcttattc
     6421 ttgtgtaagc atatcccggt gctttattat tgaatacttt ttcaagctgc tcagccgact
     6481 cctgcgcaaa ggcactcacc tgatatatgg gagtgagcgt tgagcctgtt gttttctcag
     6541 gtccaaatcc gccatggagc aattttgtat tgaatttcaa aggaaacgcc ccctgttttc
     6601 ttattatata gctaccaccg cacgcctgtc gaggtgcgat gttctttgtt actatagata
     6661 ttatacaaca taaatcatat aaagtcaata ggattactat agatttaaca tttcttttaa
     6721 aatatgccct tatttttatg aaaactcttg acagtttgct caaaaaagta taaaatatat
     6781 tatgttgata ggattactat gaataatagg acannnnnnn nnntgtcatt tctagggagg
     6841 tgcagaaatg aaaatttcaa caaaagtaga atgtggcata attgccattg cagacattgc
     6901 aatgaactca gaaaacggag aaacagtgac agtatacagc gtggcagaaa gacgtggact
     6961 ttcaggaaaa tatcttgagc aggtgcttac ttctctgagg caggcaaggc tcataaaagg
     7021 cataaaaggc tctaggggcg gatatatact caacaaaaag ccggagaaga taacactcaa
     7081 agaaataatc gacgctctcg acccatcggt gcttggaagc gatgttgcgg tagtaaatga
     7141 cgaaaacaca gaatttgcac aggtgcttga cgattatata tggctgagat tagagaacaa
     7201 gctgaaaagc tacactgagt ctatcaccct cagacagctc gttgacttct ataataaaaa
     7261 gtcagtagag aacgtttcag agcctatgta ttacatctga ttgacaaaag tcggttttca
     7321 ttttataatt agggacagag agtgtatact atatataaag gatgtgtttt ttatgtatga
     7381 cgttataata ataggcagtg gtcctgcggg gcttactgcg gcgatatatg caggcagagc
     7441 aatgctttcc gcactggtgt gcgaaaagga ctacatgggt acaggtcaga tatctgttac
     7501 agacagagtt gagaactacc cgggtcttta cggcataaac ggctatgacc ttggagaaaa
     7561 gttcagagag caggctgaaa ctcttggtgc tgagttttac gagggtgagg ctgaaagctt
     7621 tgagaaaaca gccgagggct ggaaggtcat attcaaggac ggctctgtaa aagaaggaaa
     7681 aacggttatt tacaccgcag gcacttcata tcgtcatctt gacgtggcag gcggtgagaa
     7741 acagcgcatt tcttactgcg cagtgtgcga cggcttcttc tataaggata aagttgtggc
     7801 tgttgtgggc ggaggagata ctgcacttgg tgacgcactt tatctttcaa agcttgcaaa
     7861 aactgtttac cttatccaca gacgtgacga gttcagagca aataaagctt tgcagaaaaa
     7921 ggtcgctgaa acagataata tcgtgcctgt tatgagcgca gttataaaag aggtaacagg
     7981 cggcgataat gcagagggcg ttacctatac gcaaaacggc gaggaaaaga acattgccgt
     8041 ggacggagtg ttctgcgcaa tcggaagcgt accgaataca caagctttag agggcgtttg
     8101 cgagcttgac gaaagcggat atgtgaaagc tggcgaggac gggatcacca ctgcaaaggg
     8161 tctttttgtg gcaggagatg tccgtactac acctctcagg caggtaataa cagcagcggc
     8221 agacggtgca aacgcacttt cttctgcgga aaaatatata atggagctgt aaaaagcaaa
     8281 acaaagcggt gtcggagcgt aaaactctgc accgcttttt ttctattcta tagcacaaaa
     8341 aaggacgctg tcaccaacgc cccaaattgc ttttatatat tcacgctttc cttttcaagc
     8401 aggctgtcta taaacctctg cacaggttca ggcgtttcat actgtgatgc ggtcaccgca
     8461 catatgctcc ttggcggtat ctgctctttt gtgttcaccc tgaagatgtt ccctgcctct
     8521 atctcagctt ttgtggtggc atactgcatg aagcctatgc caagattgtt ctttaccatt
     8581 ggcattacaa agtttatgga cgtaagctca atatcaggtc ttaacagcag gttgtgctgt
     8641 ctgaatatgt ggtcaagata gtttcgggtg gtggtggtgt ttttaagaca gataagagga
     8701 tatttcacaa gctcatgcag ggatatctcc ttgtctttaa gatatgaaaa accgtttcct
     8761 gcggcaacga tatcctgaag cttctttatg acgtttatgt taagccccgt tttgtctgca
     8821 aacggcgagg taactaccgc cacgtctatt agatttgccc tcaacgcttc tatagcgtca
     8881 ggggtggaca tactgtctat ctttatctgt atcttcgggt gttctctgtg aaactctact
     8941 attcggtcga taagaaagct ttgcagtgcc acctcgcttc ctccaagcct tactatcgcc
     9001 ctgtcacgct cgcttatcag tgcaagctcc tcctctgccg agaatatatg cttgcaggct
     9061 atggatatat gcttaaagag catctctccg cccgttgtca gctctacacc tttttttgac
     9121 cgcacaaaaa gcttgcaccc aagctcgtcc tcaagatttt ttacgcatcg gcttatgctc
     9181 ggttgcgaca aaaacagata gttcgccgcc attgttatgt tctggaactt tgccacataa
     9241 tagaatatct tataatgttc gaaatttatc tctctcataa accgttcctc tcctctctat
     9301 ccatacacta tgagtatgtt tacaatagaa aatatctatt tgacatatgc ttatgattgt
     9361 attataatat attcgtagac aaaaagcaag tcttacatca ctgcgcccat agggggacgc
     9421 atacaaaaaa tagttttatc atgataaaat caatgacgat tgcattcgac cgctgggggt
     9481 aaacggtgta gaccttatta tacccttatg aggctggcat tcttattggt tttatttttt
     9541 tggaggtgat aaaatgctgg agagaatttc cgaaaatctt tccgaggcta tgcaaaacaa
     9601 agtagctttc aggataccga ttttcggagg catacccgtt cctgtgtcct gcgtggtaac
     9661 atggataata atggcgatac ttgtgatagc ttcaatactt ctcacaagaa accttaaact
     9721 tatccctgac aggaaacaga tgatgcttga atcattcgta ggctttctca gagatttctt
     9781 cagagaacag cttggagaaa agggtatggc gtattttccg ttccttgcaa ctgtcattat
     9841 ctacataggc tttgcgaaca tcatcggtct gctgggcttt acaccgccta caaaagactt
     9901 gaatgttacc gcaggacttg cgatcatgag cattgtgctt gtggaggttg caggaataag
     9961 agcaaaagga acgaagggct ggctgaaaag ctttgccaaa cctgtggcga taatcgctcc
    10021 gctgaatgta atggagcttg ttatcagacc gctgtcgctt tgtatgcgac ttttcggaaa
    10081 cgtgcttgcg tcttatatca tcatggagct tatcgacgcc gtttgcccga tagtgatacc
    10141 gctggcattc tcggcatact ttgatatttt cgacggcttc attcaggcat atgttttcgt
    10201 gttcctcacg tccctgttca taaatgaagc aatagaggac gacgactaag ataacagaaa
    10261 ttcggaggta tgatctatgt tagtagcaat cggtgcggct gtagccgtta ttacaggaat
    10321 aggtgcaggt atcggaatag gtatcgcaac aggaaaggca acagagtcta tcgcaagaca
    10381 gcctgaggct aaaaaggata taagaagctc actgcttctc ggctgcgcac ttgcagaggc
    10441 aacagctatc tacggcctta tcgtagctat ccttatcatc ttcttcctga agtaagagaa
    10501 taagcgtaga aaagtaaaag aataaaaacg gaggtatata tcatgttagt agcaattggt
    10561 gcggctgtag ccgttattac aggtataggc gcaggtatcg gaatcggtct tgcaacagga
    10621 aaggcaacag agtctatcgc aagacagcct gaggctaaaa aggata
//
LOCUS       DAWEMR010000086        10635 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2620_length_10635_cov_9.955577, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000086 DAWEMR010000000
VERSION     DAWEMR010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10635)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10635)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10635
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2620_length_10635_cov_9.955577"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    548..557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4366..4375
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9160..9169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3021 a         2417 c         1921 g         3246 t
ORIGIN      
        1 gccctcccca agaagaagag gagtgtttgg cataacaagc actacctttg cgccagccac
       61 agtttttgaa gcgatataat catctgtaat gcctgcgcag atagaaacta tgaccgtatc
      121 tttcgttacc gcaggagcta tctcatcaag cacctcgtca agctgctgag gcttaattgc
      181 aagaaaaaca tattctgact tttcaactac ctctgcacag cctgaacatt ttactgttcc
      241 aagctcgtca agtctatcaa gtatcggagc atatttatca aaagcgtaaa gctgaatatc
      301 cttactcatt ttactgcctg caatgccctt catgatagca tagcccatat ttcctgcacc
      361 gataaaacca acatttatca tacactcaag acctttctga ataaaaggca gaagcctgcc
      421 ttaaaaataa cttaatttta attatacact aaatctcaac ggatttcaag tgtttcacct
      481 aaatttcaca ttagtacccc atttttattg tatattttca cgcaaaaaac agcgacattt
      541 ctgccgcnnn nnnnnnnatg tatttgtcgc tttatgtcca aactgtgttc ttactttttc
      601 ataagcaaac ttcacagcag gcaggaataa catgagcaag atcagatcca cagggagctg
      661 gataagattt ttcgctattc tcggtgtgca gtatgtaaga aaaccgctca ttccttcttt
      721 ggtgaggttt gtgatgccaa aagtcttgtt tacataaagt gtatacagga aataagaatt
      781 aagtcctata ttacagaaaa gtataacact tcctcttgca attgcgatcc ttatgtagct
      841 ttgaagcttt ttgttgttaa aatcgcactt atacaacaaa cagccgtaga tgatacctga
      901 aataataact accattgcaa gctgtggact gtaggctctc ggagatttgt tctgcactat
      961 atatcctaga aagtcaagca caaatgtaga cattccacat accacaggtc cgtaaagcat
     1021 acctatagta caggttgcaa gaaaagtgaa accgatcctc aagtcaggcg ttacctcaat
     1081 ggaaaatgat ctgatagcaa acgccaacgc tatcagtatt cctgctgttg ttaatgttct
     1141 aagatctttc agtgacttga aagagtcata gaacattctt ccgaagctct tcataaattt
     1201 acctccttat tctgcgagct tgtcgctttg tgcataacgg agcataaaaa cagctctgca
     1261 cactacccat gcgggatgtg cagagccgct ataaaaagct tcaattacac attcagcggg
     1321 atgcgaaaac cgaaccgcaa aggttctgcc tttttcgttc cgacggcaac tccccgtccg
     1381 acggacactt aacgctcagt tttctactct gtaatatgtc aaagtcttaa agactcagac
     1441 ctcatgaaaa attagaatgc agtttcattg aggatcttgt aaagctcttc ctcgaaaggc
     1501 ttcttcattg aaagagcctc ctgaatatct acatcatata tcttgctgtc cttcatacca
     1561 acaactctgt tgccaatgcc ctgctctagc agttcaacag cgtgatagcc catttcagtt
     1621 gcgacaactc tatctcttac tgtaggagag ccgcctctct gaacatgacc gagaatagta
     1681 gctcttgact cgacaccagt aatatcctga agagttcttg tgatatactc tgtcttgcca
     1741 acaccctcag caactacaat gataaaatgt gtcttgcctg acttctttgt ctgctccatc
     1801 ttctgagcaa tagcattgag gtcataaggc ttctcaggtg tgataacagc ctcagcgcct
     1861 acagcagcag caacgttgat agcaatgtga ccagcgtttc tgcccattac ctcaactact
     1921 gaacatctgt catgtgactg tgctgtatct ctgatcttat cgatcatttc catagctgtg
     1981 ttcatagctg tgtcataacc gatagtatat tctgtgcatg aaatatcgtt gtctatagta
     2041 ccaggcagac caatgcatgg gataccctgt gctgaaaggt cagccgcacc tctgaatgag
     2101 ccgtcaccac cgataactac gataccgtca agacccattt caagacactt atctctgccc
     2161 ttctttacgc cctcgatatc tctgaattca ggacatcttg cagtataaag acaagtaccg
     2221 cctctctgga ttatgcctga aacagttctt gcattcatct caataacttc accattaaga
     2281 agaccgttat atcctctgag gatacctacg acttccatac cctttgcaag accagctctg
     2341 catactgctc tgacagcagc gttcattccc ggagcatctc ctccgctggt aagtacacca
     2401 attttcttag ccatgtaaaa tacctccaaa ccaggaaaca atttatcctg ttactttcct
     2461 aataatctat aatgagcata gcacaaagcc cctgaattgt caagggcttt gtttgttttt
     2521 taacatttta ttaaacgttt tcgttattca gcaggcacaa cagccggaac tggtgttgtt
     2581 tcagaagagc tgcctgtgtt cgtatcaggc ttaggctctg ttgcctttgt tgtagtggtc
     2641 gtagtagtca caagacctgc ggaaactgta actgtaagca catcaccatt tttaacattc
     2701 acagtgtttc caacattaac gcttgtcgcc ataacatatc ctgcagaaac agagcttgag
     2761 aaatcggtaa cgaccttata cttgatatta taagaatcaa gcagctcaag gtaagccttc
     2821 tttgacaggc cagagaaatc aggaacagga acattttcag ggcctgcaca tactttaaga
     2881 acaagctcat ttttcaaaga cggatcaaaa tcagtgccct ctggtatgct ctgctcttta
     2941 acaacgtcct tatcgtcaac atcagaataa taatattcag ctttgatagt aaaatcctca
     3001 ccaagctgtt ttacaacagt attgtattcc agacccacaa cgttaggcat tatagcgcca
     3061 tagccatatt ctgactgact ttcagcatca gtcaccttag gcgtagcaac tgtggtgctg
     3121 taaacgaata cctcgctgtc aactccatct gcatcatcag aagaaatgac cttatcctca
     3181 gacgaatcat tattgctgtc cgacggacct gaaaacaact catatagcag aaaaactgaa
     3241 agcaaaagca ctataacaag caccgtccag cctattatcg tagccgtttt attcttaggc
     3301 ttttcttcct cacgagtctg ctgtggcatt tgccttgact gcgttctttg tcttggaaca
     3361 tgctgtatag gaatagtctg agttgaaccc ttagggtgtt ccatatgcgt tgtatgctgc
     3421 tcaaaaagtg cagtaacaag ctctgaaact gtctgtattc tttcttcacc acgaactctc
     3481 atagctcttg aaagaactcg tgagatatgc tgtggtattc tcggattgac tctgcaagct
     3541 tcgaccatag tatcattctt tgtacgggta taagcgtcaa ggggcataca gcctgttagt
     3601 attctataaa gaactgcagc aagtgcataa acgtcagtcc atgtgccctg ccattctaac
     3661 gaagaatatt gctcaggggc ggtatatcct gaatagaact caggagaaag ccctgtattg
     3721 gatgttctta tagaacttat gcaaaagcct gtaagtttaa gctcacaatc agttgtaaca
     3781 ataatatttt caggagatat tcccctgtgt atgatacctg cattatgtat aatgctcaat
     3841 gttgtaaaca atggcacaaa aagctttttg acctgttccc atgtgagaaa gcctgtattt
     3901 gactgtaaaa atttctttag cgtcacgccc tccacatatt cgagtatgac ataggtcgta
     3961 ttattctcgc aaaacatatc ctttgccgtg gcgatatgct gaagatttct catgcgtgaa
     4021 agaactttgt taacgtctgc aaactcagac ataaatgtct tatacttagc cagacaatct
     4081 ggattcacca caagtcgctg actttcgcta tctctttcgc aaagagtgtc aggcatatac
     4141 tctcttgcca cacacttgca tttgccaacc atatcgtagc aaatgtaaga cgcaccctca
     4201 ccattatatg aaagcatttt tccgactata tatctgttgt caagcactgt tctcggtgca
     4261 agataagcct gtaaatgcgg gatatcgtcg gtatagctac aataatgaca gcgaccgtca
     4321 gaaccaagtt cgttcataca gcccatacat aacttagtac taggannnnn nnnnngaacc
     4381 tcctttttac gttattcatc aatttatatt agcaacaaac cgagcataag tcaagttttt
     4441 ttgtgcaatt gtagaattta cgcaacaatt tgtatttctt ttgtaacatt atgacccttt
     4501 tacagatact caggataaat aagcgtagaa gaataataca gatacttatt atccgtatcg
     4561 aacgttacat taagaaccac tggttgttta tcacctgcgt cattatcata ccaatagcgg
     4621 tatatataat ttttagtgtc ttttgaccat acgatgccgt aatattcaag gtctatggta
     4681 tctgacaagc cactccactt tgcaccttgt tcaaacttat caaggttgat agctttactc
     4741 tttttttcac cgtttgcatt gtttttaagc accttgaaat ttgaatattt tacggtttgt
     4801 atacgttctt catcatcaac agtaacaagt gccgcccatt caggataatt tatgccatca
     4861 acattaacac tgtcatcaga ctcaatgata taatcaaaat tcagtgcatt gttcactccc
     4921 tgaaaagccg attcgtcctt aaaatgcata tcaagctttt tctgagcctt tgcaacatct
     4981 acgccgatat attcagaaat gctctcagcg gtgttttcac cctttccgct tataatattc
     5041 acaacgataa ggacgacaag caccacaagg agcactaccg ccactgttat aagaataagc
     5101 ttgagcttgc tttcagacaa tttatgtttt ttctcaccca ctatttccac atttagcggt
     5161 ttattctgct ttttgaaacg cttcatgata gatgtatttt tgtctggtct taatcttgtg
     5221 tcacagttag gacagaaaat gtcatcatcg tcaactgact tgtggcattt aggacactcc
     5281 ataaaaacaa ctcccttttg caacatccgt gcatttgcac accttttcaa aacaagtata
     5341 ccataatctc tgtaaaaagt caacaaacgc aaaacagcgg acagaagtct atccgctgta
     5401 aaaaacatat atcagaagtg catctggtca gggtcttcga tatcctttgc aaaacagcct
     5461 gcaacaggta tcgttttgct gcgatatttt gaaaggcctg ctgctgtctc atcgtcaacg
     5521 ataaacgcct tatcaacaac tgcattcttc gctttaagtg tcatgacctg aacgcctata
     5581 gtatctctcg tggacttagg cataagaagt gcagtatcaa atatcattgc tctgccgtta
     5641 gttgtgcgaa gcattatctc agcgttatcc tgcacaaaga acattcccac aagcttagcc
     5701 tttgcagaat aagcgttggc aagcttcttt ctgttggtct ttgtttcata ggcattgagc
     5761 ggcacttttg ccacctttcc gttttcaaaa cagaatatca tatagcccga ataatctaca
     5821 gtattcacca tggacacaac gccctcaccg tcatcaaaac caagctttgc aggaatatag
     5881 tcgccaagag agcttgcctt tgtgtcggca aactgcgata ccttggtctt atacacagcc
     5941 gccttgtcag agaagaacag aagctccgtt ttgttggtgc tttccatttc ctgaagcgga
     6001 ccgtcgccct ctttatactt ctgctcgctt gccattctca gtgattgcgg agtacacttc
     6061 ttgaaataac cgctttgaga taggaaaagc ttgcaggcgt agtcagggat atcgtcctct
     6121 atgtctatct cctcgatatc agtcttataa tagaactgag tttttcttgg ctgaccatac
     6181 ttctttgaaa cttcctttaa ttcggaaatg atgatattct ttatttttct gtcgcttcca
     6241 agaatatctt caagctctgc aatagctttt tcaagatcct cagtttcggc agttcttttg
     6301 aggatatact cacggttaag gtgtctgagc tttatttcag ccacataatc agcctgtatc
     6361 tcatcgatac cgaagcctat catcaggttt ggaacgacct ctgtttcttc ctctgtctcc
     6421 ctgattattt ttatagcctt gtctatatca aggagtatct ttccaagacc tttcagaagg
     6481 tggagcttgt ctttcttctt tgcaaggtca aagcttgttc tgcgtcgaac gcaatccatt
     6541 ctgaacgcta cccattcttt aattatatca taaacgccca ttattcttgg atagccgtga
     6601 acaagtatat agaaattgca tgaatacgag tcctgaagcg gcgtcagctt ataaagcttt
     6661 tgcataagct tgtcagggtc tacgcccttt ttgatatcta tacctatttt cagaccgtta
     6721 atgtccgttt catcacggat atatgatacc tctcttatct tgcctgcctt aacgagagca
     6781 attatcttct cgattattgc ttcaactgtg gtagtggcag gtatctcaac gatctcaaga
     6841 caccttgact ccttgtcata ctgatatttt gctctcagct taacagagcc ttttcctgtg
     6901 cggtatatct tatcaagctc ctcctcatca tagagaagtg aagcaccgcc cggaaagtca
     6961 ggtcctttca gcgtttcaag agcgttgaaa tcaggatttt tcataagtcc gatagttgtc
     7021 tcgcaaacct cgctgagatt gaacggacaa actgatgaag ccatagaaac accgatacca
     7081 acgtttgcat taacaagtat tgacgggtaa gtagcaggaa gcaaggtcgg ctctgtggtg
     7141 gtgttatcat agttaggaac aaaatccaca gtgtccttgt ctatatctcc gaaaagctcc
     7201 tgacagatag gcatgagctt tgcctcagtg tatcgagagg cagcatacgc catatctctt
     7261 gaatatgcct taccgaagtt acccttactc attacatatg ggtgcaaaag cgcttcattg
     7321 ccctgtgaaa gtcttaccat ggtttcatat atagcaccgt cgccgtgagg attgagcctc
     7381 atggtcgcac caacgatatt ggcagacttt gtgagcttgc cgtttaaaag tcccatttta
     7441 tacattgtat aaagcagttt tctgtgtgag ggcttaaagc cgtctatctc agggaacgct
     7501 cttgatacca aaacgctcat ggcatatggc atatagttct tttcaaggga cattgttatc
     7561 ttttcatctg caacaagtcc tgcaccctcg atatatgcgt tgttatttgg tttagcagtc
     7621 tttttaggct ctgccttttt tcttgccata aaatctatcc tttctgcgcc gaatatattt
     7681 tcagatgcaa attatgaaat atccgccata tcaagataat caacaccatt attggtgatg
     7741 aaatcttttc tgccctgcag atcgtcaccc aagagcatat cgaacatcca ttgtgtctgc
     7801 tcgatatcgt caggctctat ctttataaga cgtcttgtgg cagggtccat tgtcgtaagg
     7861 ctcatcatct ccgcctcgtt ctcgccaaga ccttttgaac gctggatatc aaacttttta
     7921 ttgcctatca ttttgagtat ctttgtcttt tcgttctcgt catatgcaaa gtaagtcttg
     7981 tcggaagtcg ttatctcaaa gagcggtgac tccgcaatat aaacatatcc tttttcaata
     8041 agggtcggca caagtctgta aagcattgtg agtataagcg ttctgatatg gaagccgtca
     8101 acatcggcat cagtacagat aactattttg ttccagcgta ggttgtcaag attgaacata
     8161 gatatctcct tgtttttgcc cgtcttgacc tcaacgccgc agcctatgac ttttataaga
     8221 tctgtgatta tctcgttctt gaaaatctta tcatagtcag ctttcaaaca gttgagtatc
     8281 tttcctctca cagggataac agcctgaaac tctgcatttc ttgccatttt tacagaaccg
     8341 agcgccgagt cgccctccac gatgtaaagc tcacgctgag aaaggtcttt tgaacggcag
     8401 tcaacgaatt tctgcacctg atttgaaagg tcgatagagc ctgcaagttt tttcttcagg
     8461 tctattcttg acttctcagc cttttcacgg cttctcttgt ttacaagaac ctgctctgct
     8521 atcttcaccg cttcgtcagg attttcaata aagtatatct cgagattgtg cttcaagaac
     8581 tctgtcatgc actccttgat aaacttgttt gtgatagctt tctttgtctg attggcatat
     8641 gaagtctgtg tagaaaacga acttgaaaca agcaccaagc aatcctcgat atcctgaaaa
     8701 gtaataggct tttcgttttt cagatactta ttgttgcttt tgagataagc atttatctga
     8761 gatgtgaaag cactctttac agcgtcctcc ggtgagccac catgttccag aaagcttgaa
     8821 ttgtgataat attcaagaca ttttacctga ttagagaaag tgaacgccac agaaagcttt
     8881 accttgtaat cgtctttgtc ctcacggtca cgacccttgc ggtcgcctgt gaaaaactgc
     8941 accgaggtca gaggcttttc acccgctatc tctgcaacat aatcggcgat accattttca
     9001 taatagaacg tcttttcttc aaaagaattg ttctcacgct gtatacgcag gataaatgtg
     9061 atattagggt ttactacagc ctgctttttc agaacgtcct caaaatattc gttctcaatt
     9121 tttatatcag tgaaaacttc aaggtcaggt cgccaatgtn nnnnnnnnna tatccatcac
     9181 ggaaaacctc gacgtccata aactcagatg aatactgcgt cgcacaagca cctagtccgt
     9241 taagacccaa cgaatattca tagttatctc cgccgttatt attatacttg ccgcctgcat
     9301 aaagctcgca gtaaacaagc tcccagttat attttttctc acgctggtta tagtcaacag
     9361 gacaaccacg tccgaagtct tccacctcga tagaatcgtc attgtacttt gtaacgatta
     9421 tcttatcgcc atgaccatca cgagcctcgt ctatagagtt tgaaagaatt tcaaaaacag
     9481 aatgtttaca gccctcaagt ccgtcagagc cgaatattac cgcaggacgc tttcttactc
     9541 tgtcctcccc cttaagggat actatatctt catttgtata ttccttttta tttgccattt
     9601 ctcatttcct ttcttacgga tctgaagaat tttttacata caaattatat tataacacaa
     9661 agtcaacgat tttgtaaata cattaattaa acatttttca gagcaaagcc gaacatttgt
     9721 tttgttacta ataaacaaaa acataggcta ttgattattt gaaaaaaata ttatataata
     9781 taggtagctt aaaaaaatcg gaggacaaaa tggatattac gataaatgca ccgcagaccg
     9841 aaagcaactc aaactctgcc aaagcaatgt cccttaacaa tgggctgata tggttcatat
     9901 gcttcgttcc gcttatcggc ttgtttcttg aaaactatgc aaactctgcc actgcaggag
     9961 cgttcctgtg gatacttgtt ccgctgttca tgataggctg ttccattgca gattgcaaac
    10021 agcttataaa gcatggcatt gatgctgcac accttttcaa atgggtatgg ctaacacctt
    10081 tttatgttta taagcgtgaa aagctttgcg gcagagagct ttacaaagca ataatgtgcg
    10141 gatttttcat tatcgctgca ctgttcatga atggttttac gcagagcata aaaatagaca
    10201 acaactatat gcttatatcc gcacagaaca gttatgtaca aagccttgac aaattttcgg
    10261 gaaattcagc aaaggtaatc ggtgagtgca tagcttctta tctaggcgag gacgcaaagt
    10321 gggattgcac aaagaacggt cacaactaca cagtaacagt aaaaggcaaa catggctctg
    10381 ataactacac tatttacttt ttaatagtat atgacggctt cacctacaga aaattcacca
    10441 tttcagacgt gataaaaaat aaagtttcac tccgtgacga tgaatttagt gctgtatgca
    10501 aagagatatt cacagaagat aaaagcgaca ccgacagtag caatgaagaa agttcaaatt
    10561 cacaaacaga ataatttcat ggggctgtaa aaaaacgcgc aataaaaaaa gactgcaacc
    10621 acccttaaaa gggta
//
LOCUS       DAWEMR010000087        10492 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2675_length_10492_cov_11.653540,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000087 DAWEMR010000000
VERSION     DAWEMR010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2675_length_10492_cov_11.653540"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2941 a         2407 c         2467 g         2677 t
ORIGIN      
        1 ggcctttgtg cgctgtttac ccaagccccg ccatattgct aggtgaaaag atcacagccc
       61 tgtgccggga acttcgtcaa aaaggtcgcc gactgcaccg tcgttgcccc agccgcaagg
      121 agcttctgta catcttccgc acaggttatt ccgcctcccg cacagacttt cagaccgtgc
      181 ttcacagcgg tataagtgta accgatcgtc aacgggagtt gccagtgacc gaagcaggag
      241 gtcgactcgg catcaaggcg gtcgcttatg tgatatatgc catccgtctt ccggagatac
      301 ggtgagcgta cagaatccag cagtgataca tacttcacac caccctccac gagcagcggc
      361 atgatgtaat ccttcggcag gttgatgttc agcttcggca tgacgggaat acgcagtgca
      421 gtgttcagtt ctcgcaaaag cgacacgata cgtccgctca gtccatccgt tccgatgagg
      481 tttcccatat agaagaaatc aagctggatg ccgtctgcac ctgctgcttc aagctgctga
      541 caggcaggaa tccattcctc tggggcaagg ctcgatgcgg tgaagcttgg gatcaggagc
      601 atttccgtct gtggacggag cgtccgaagc atttccagac actcatccaa tgtcaatatc
      661 tcagattcga agggtgcaga tgcgtaggtg aacccaagtt tcttatcgac ggcaaattct
      721 cgtttgccgg aaaccctacc acgctcatag tcggcgcatg atttcagaat cgcgccctga
      781 acaccgtttt cagcaagccg cagcacattg gaaacgcttt cagttgcgat gcaggatgat
      841 gcgatcagcg ggttttcgag ctgctttccc atgaattcaa tcatgattct ttcctccctt
      901 tttgatcttg gcatggtaga agtagttgac gaacggtacg gcatagtcat tggacagtgt
      961 cttgatctcc gcttcgagct gctcccagta ggacaagtca ccctttcggt agatcgcctt
     1021 gtagccgtca agatgctgcg gatacttctc cgcaatataa tccagaattt ctcccttcgc
     1081 tgcgccacgc aaattcaggt tctcgaaaca gatttggtcg acatgaccgt taactgcttc
     1141 gataagagcc cgaatatccg taatatacgg gaagattggc gaaacgaaca gatatgtcgt
     1201 gatccccaca tcgtgaagcg tttcaagtgc cttcagacga cgctgcacag agggagcccc
     1261 tggttccata tccttccgga atccgtcatc aagagtattc agtgaaatgc ccacggacaa
     1321 gtccggaatc tgccggagca gatcaagatc ccgtaccatg agatctgact tgctcaggat
     1381 ctcgatatgt gcgtcagttc ctcgcagccg ttcgagaact tcccggctct tgtggtattt
     1441 cgcctcaaaa gggttatagg ggtcggtcac ggacgaaagc agaattgtct taccagtgag
     1501 gaatttcggg agcttgtcgg actcgtattc cttgacatcc agaaacgttc cccagtcctc
     1561 gtcatgacca gtgaaacgtt tcatgaaaca tgcatagcag tagcggcact gatgtgtaca
     1621 gccgccgtag gggtttgcca catagtcgga tgcaggaaga ttcgacttga cgaggatgga
     1681 cttagccttt aatgtggata ttttcatggt gattctcctt tctggaaatg agcgccattg
     1741 tagcaacggc gagcagcgca ctgacggaac cgaataccag catgatctgc ggcgagttac
     1801 tgccaaaaac cgcagccaca ccaactgctg tcagaagtcc cagcgtatga ccgatattct
     1861 cggcaatcgt catggaatcc ttctgatagc tacctgcggc tttggctctg gcagaaatgg
     1921 tgtagaccac accgccaccg aagccggtca cgagccagag taggatgaac aacggcacag
     1981 atgtgacaaa gtgcatcgta aacagcagaa tactgatgcc aaggtgtccg gcgtagaaca
     2041 cgggcaggat acggtttgtc aaacgggaaa tgatcggctc cacggacata tatgtcaccc
     2101 acgttccaca gaacagaagc gcagcataca caatgccgaa tgtgctgtta tgcgagagca
     2161 gcaggctcat ggcagcgaga gtgatatgtg cgtagaagaa gtagtgaagc tgatgaaatg
     2221 tcatcacgag tgaaaagccg ttttgtcctg tcatgcgtgt gatgtcggat ttacccttgt
     2281 actcatgcag tgccaggaaa gcaacaatgg acgagagcac cagaatcgct aagggaacta
     2341 ccactgcaag tggtgcaagg gcaaatccac ccacacggaa tacacgcttg agtagacgat
     2401 taccgtcgct ctttagcccg ctgcgcacgg actggatcac tgccgagagc agagacaaag
     2461 caatgcacgt cagcacaaac gcagcctttt ccgaaagctg cactgcaaac gtgaggaact
     2521 ctgccatggc agacagaaaa cacatgatcc gcagtgtact gcggctgagt gagaacggct
     2581 ttgggaaaag gttgcctaaa tggtaggcaa gcggataaca aaggatctgc cagagcggaa
     2641 gtgaatgcgc cgcaccgaag gcgatccacc cacactccag aacacctgtc agcagactta
     2701 gcaaatagaa catcaagcaa cactcctttc aggagtactg ccggatccaa gttcacagca
     2761 gtgctcacag tcggaccagt aacgtgcctt ctcaccgttc aggatctcca ggagggagtt
     2821 gtccttcaca ttgccaagga gaaacttggg gtcaacggct ccggtgctgc tgtcaagtgc
     2881 ccaaaagcag gcaacaacat caccgtttgg aagaataccg atcgaatgac gcacgcagcg
     2941 gcagatgttg gtatacttct tgtgacccgg catgaggtag tggaagtcca cctccggctt
     3001 gtgaccaaca gggagggaat ccaccatttc ctgcaccatg tggacatacg cctcacactg
     3061 tgcatcggtc atggcagcct ccggatagtc ggcacctctg ccggacggga agaagcgcag
     3121 gatgctccag ttgtccacgc cgttagcaca agcccacgca aacacagcca gtgcatcggc
     3181 atagctgctg ttgtatgtac ttgcgacggt ctggagccca acctcgcagc ctgcttcttt
     3241 taacagtgca actgcttttg cggcagccac gctataagca gcaggacgaa gcttataggg
     3301 atggtgcgga acaacgtcca tggtcatctc cacatcgtgg acagtactgc cgagaaaatt
     3361 ggcaagttta ctgtcaatgc catagccgga gcagctcaca ccgagcttgg tcctgccaag
     3421 tacttcggac agagcagtca gtagctcagt gtgagcggga atatcggtga acagctcgcc
     3481 tcctgagaga tccatgcgaa cttcatagcc gttctcctgc aattcacgta cctggtgagc
     3541 aacattgagc ttctgttcct gagtaagctc gttgtttctg caagacttgt ccgaggttgc
     3601 acccatacag cagatggcac aattccaggg acacagagcg gtaacatcat aaatgataga
     3661 cagctttttc atagttattc ctccttaaag ttggttctgt tttggggtgg cttttgctta
     3721 caaacttatt atagcacaat atagggatag cttcaattga catcgaaatg aaattctgtc
     3781 agtaattcaa ctgaatattg acagaatata aggttttgtg ctataatatt gtcagttgga
     3841 aactgacaat acagctgagg aggattatag tatgggtgaa tcatttgacg caggaactct
     3901 ttcacgatat gatatgagtg acagtacata tcgccagctt cagcgagatc atatcagatt
     3961 tgaggaaaag tacgccgaga aattcagcaa tggctttatt tgcagaatac taatcaatta
     4021 tatgtgctat gctcaggaaa cctttttaga acgatttgaa agcgatatgg tcacaagttt
     4081 ttccggttca gaacatgacc aatacataaa agacgaccgc atcagcttta ctaaacaact
     4141 gacagaaaaa atagctgagt ttgacttctc aaccaatacg tcatttaaga aacgcatcgt
     4201 aataacatat ttattggaac aattcacttt gctacctctc tcagaacgag aaatagttta
     4261 ttgttatcat caatatacat ccattaaaca ggctatggat aaaaaagaaa tccttattgt
     4321 aaaacaactc accggaaaag aattcgaggt caagccgttt gacatcagaa tagacgaaaa
     4381 cacgctatca tactatctta taggttattc aagactgaaa ggaagtagtg gtggttttga
     4441 aagccattca ttcaagttaa gccgtattaa agaatgccga tcaaaacaca aagaagccat
     4501 actatcatat acagaaataa aaacaataaa agaaatcaat gataaatttg gcagtgcata
     4561 cattcctaga aatctcataa aaaaggatat agaaaaaact atcgtgcgct taactaaaaa
     4621 tgggtatgaa aatctatatc tacgaattat ttctcatcaa agacctatcc cggttacacc
     4681 tcctaagcag atcagtttag agggggagga ttattatgac ctaacttttg attgttcgta
     4741 tcagcacatt aggaactact ttttttcgtt tggtgctgaa gctgaggtta ttagcccttt
     4801 atggctgaga gagaggttta tggctgatta caagagatcg ctagaaagat atagtagtga
     4861 ttagttttta cacgaacgaa aaagacagat acctcacatg aagtatctgt ctttcacttt
     4921 tatagcccgc tgtataggtc tttcatataa attcttgtaa aacttctata ttagtaccga
     4981 ctttacgctc ggagagcttt ttttgcattc acacatctct gataaccgca cccctactgt
     5041 aggggcgaac agcattcgcc cgtttcgttc cccccactca acttcgtaaa aaaacaaaca
     5101 acgaagctcc ccaaaacggg acgtcaattt ctgtgagctt gcgagcagaa atttcgccgc
     5161 tcccctacaa atattcggga tggtttctac cgcttgttcg cttcgctcgg tgctttattt
     5221 tactttatgc ttgtaatttt ctttggtctg ctatgctaat tcctcattcc tcattcctaa
     5281 ttcctaattc ctcactcctc actcctcact tcttccgccc ttgtgcaatc tgctttataa
     5341 caagtctaaa ctttgttcac attcacgaaa gaaatttttc tttccgccct tgctatttat
     5401 gtgaaaatag tatataatta tcttatgcgg ttgatatatt ttgtgtaaat gatacatata
     5461 attatactgc atatcacatt aagccgtcca acggcataga ttggagcgat caacttgaaa
     5521 aaatacgaca gcacaaaaat aaaaaatatt gctattgcag gtcaggcagg ttctggaaag
     5581 acctctctga ctgaggcact gctatataaa agcggagcta ctgacaggct cggcaagata
     5641 actgacggaa acactgtttc tgattatacc cctgatgaga taaagagaaa atgttctgtt
     5701 tacacatctc tttcttcttt ggaaactggc gagttcaagg taaatcttct tgatactccc
     5761 ggtatgttcg attatgaggg cgaagctgtt gagggcattg ccgcaagcgg ttgcgtactc
     5821 atcactgtat caggtaaatc aggcgttcgt gtaggtactc acaaggctta tgaccttgca
     5881 aaggaaatgg gcaagcctac aatgttcgtt gtaacaaagc ttgatgacga taacgcaaac
     5941 ttcaataacg ttcttactca gctcaaagca gagttcggac ctacagtttg ccctgttgtt
     6001 gtgcctgtta tcgcagacag aaagatagtt tcttatgtaa atctcattga gatgaaagca
     6061 tataagtatg aagacggcaa ggcagttgaa actgatatgc ctacagctga tgtatcagag
     6121 aaaatgggtt atcgtatcga cggacttatc gaagccgtgt ccgaagccgt tgcagaaact
     6181 gacgaggcgc tttttgaaaa gttcttctca ggcgaagcat tcacacagaa agagcttatc
     6241 gacggaatac atagcggcat gaactcaggc attataactc ctgttacctg cgtttcttcc
     6301 acagacctct caggtgttga tatgctcctt aaggagatag acctactgct ccctcctccg
     6361 tcagaaaagg acgctatgat aggcgaaaca gcagacggcg aagctgttga ggtcgcttgc
     6421 gttgaaagcg accctatgag cgcatttgta ttcaagacag ttgctgaccc atttgttggc
     6481 aagatgtcat atataaaggt attttcagga aagcttacac ctaacaagga ggttgtgaac
     6541 gctaccacag gaagcaccga aaaggtcgga aagcttgtta cgcttcaggg caaaaagcag
     6601 atagacgttg ccgaagcagg ctgtggagat atcgttgttg caatgaagat gaacgtgaac
     6661 accaacgata ctatctgtga ctccacgaga gttgttaagt ttgcacctat gaccttccct
     6721 aagccatgct acaaaatggc agtaagagct aaaaagcagg gcgatgaaag caagatatcc
     6781 gccgcaatga caagacttat ggaagaagat cttactatag attacaagct cgatccgtcc
     6841 acaaacgaaa tgatactcag caccttgggc ggacttcacc ttgattctgt tgtgggcaga
     6901 cttgcaggaa cattcggcgt tgaagtagaa acctccgtgc ctaaaatctc ctatcgtgaa
     6961 actatcagaa agaaagtcaa ggtgcagggc agacataaga agcagtcagg cggtcacggt
     7021 cagtatggtg acgtttggat agaatttgag ccatgcgtat ctgacgacct tgtatttgaa
     7081 gaaaaggtgt ttggcggagc agttccaaag aacttcttcc cagccgtaga aaagggcttg
     7141 caggaaagcg taaagaaagg cgttctcgca ggtttccctg tagtaggtct gaaagctatc
     7201 cttgtggacg gctcatatca ccctgttgac agctcagaaa tggcattcaa aatggcagct
     7261 tctatcgcat acaaagaggg tatgcgtcag gcagagcctg ttctcctcga accgatcggc
     7321 tccttgagcg ttttcgtacc tgacgataac actggcgaca tgatgggcga gcttaataag
     7381 agaagaggca gagttctggg catgaatcct gcggaaaaga agggcatgac cgagatagtt
     7441 gccgaagttc caatggctga aatggcagac ttcacactgc ttctgagaca aatgacacag
     7501 ggtcagggcg tgttcacctt tgaaaaagca cgttacgagc cgcttcctgc aaatcttgtt
     7561 gcagacgtta tcgcaaagaa cgcagtggca gattgatttt ttggaataga atttttaagg
     7621 gataattata gaattgtgat aaggacagta cgggaatgtt tcgggcgttt ccgtactttt
     7681 ttgcacaaaa aaacagacgg aataaaaacc gtctgcaatt atcatatgta tttgaataca
     7741 ccatagtagg ggcgaacagt gttcgcacgt ttcctgtcag cttttatgtg acgcacatat
     7801 cacccattcc aatattttcg gtcaaggctt cgatactgaa cagccattgc gatatgcttt
     7861 ttgtctatga cctttgcacc gtccatatcc gcaacagttc gtgcgacttt aagtatgcgg
     7921 tcataggctc ttgctgaaag tcccagcctg tcaaaaacgt tctccaaaag ctttgaagcc
     7981 tcatctgtca cagggcagac ctcgtgaaga atatcagagg ttattcgtgc gttgcaggtt
     8041 atgctagtgc ctttgaaacg ctcgttttgt atgtcacggg ctttttgaac acgctctctt
     8101 atggacgctg atgactcctc tttggcagtg gaagaaagat ctgcaaactc aacaggtgca
     8161 acttccaaat gaatgtcaaa acggtcgaga agtggtccgc tgactttgga aagatagctt
     8221 gaaacttgtt tcggagagca tatgcacttt cttgttgggt gaccaaaata tccgcatgga
     8281 caaggattca tggcggcgat aagcatgaac gaacacggat atgttataga ccctgacact
     8341 ctggatatgg tcacctgcct gtcctccaaa ggctgacgta agatctcaag ggcagggcgt
     8401 gaaaactctg caagctcgtc aagaaaaagc aagccgttat gtgcaaggga tatctcaccg
     8461 ggctttggca cagagcctcc gcctgaaagt cctgccggtg aaactgtatg atgcggtgaa
     8521 cggaaaggtc tggcggtgat aagaggactg ttgctgtcca aaagtcctgc cacagaatgg
     8581 atatttgtgg tttctatgga ctcctcaaag gtcatggcag gaagtatcga cggcatacgt
     8641 tttgcaagca ttgattttcc tgagccaggc gaacctatca taagaacgtt gtgaccgcct
     8701 gccgctgcga cctccaatgc tttttttgcc gctgattgtc cttttacgtc ggcaaaatca
     8761 agacttccga aataactttc agcggtgggc ttataatgtg ccacaggaac tattgccgcc
     8821 tttttctcaa agtgaaaaac aagctctcgc aaagacctta cgccataggc ttttataccc
     8881 tccacaacgg aagcttcacg aacattgtcg gcaggaacat atatctcaga aagtcccatt
     8941 ttctgcgcca ttatggtcat aggcaaaacg ccctgaacag gtcttacttc tccgctgaga
     9001 gaaagctcgc cgataaaagc ggaggtggaa agcatactgt catcaacaag gtcagccatt
     9061 cgcaagagtg caactgccac ggccaagtca tagactgagc ctgtttttct cacatcggca
     9121 ggtgcaagat ttatgattat ttttctcaga ggcaatgtta tctcgcttga acgcagtgca
     9181 gaacgcacac gttcacggct ttcttttaca gatgcgtctg caagccccac aatatcgaaa
     9241 gagggcagac cctttgaagc ttctatctcc accttgaccg caaaggcatt aagccccagc
     9301 agacctacac tgtttattac agcaaacacg ttgacttctc tcccttacag cttaatctga
     9361 acacatttca gagcttatga tatcgagcag aaaatctgca atatcattat acacctcacg
     9421 gcggttggtt tcgttcacaa gctcatgacg tgcattttcg tatatcttca tctcaacgtc
     9481 acagccgttg agggacagcc tgttgaacac gcttagtacg ccttttccgt agtttcccac
     9541 agggtcggca ctgcctgcta taagataggt aggcaaagtt ttcggatagg tggagaacca
     9601 cttttcgttt gaaacatacc acaaaagctt tgcaagattt tcaaagccgt ttagggtgaa
     9661 aataaagttg catttagggt ctttttcgta ggtggaaact atttcagggt cacgggatat
     9721 ccaatcatat tcgctgactt tatcggtaat ttttcttgta taagcaccga aagcaagctt
     9781 atttacagcg ttgcttctat atttgtcacc atgaagaatt ttcaggctgt caacaagggt
     9841 gagcaaagca gggatacctt ccatgccgcc gcttgttcca cagaagatag ccgctgtgag
     9901 gtttctgcca tgcttggtga caaaagctct tgcaaggaag cttcccatgc tgtggccgag
     9961 aagaaaatac ggcttgtcag ggtagttgtg tttcatcatt tttgtgagag tatacatatc
    10021 ctcgacagcg ttttcccagc catgttctgg ggagaagtag ccaagctcgg aattatcgtt
    10081 cacactgtcg ccatgaccga ggtggtcgtt tccgcatact gcgaagccca agcctgtgag
    10141 gaactcagcg aggtcgtcat aacgtctgaa atattcgcac ataccgtggg atatttgaac
    10201 gacacctctt actgtgccga tagggaaacg aacgtaatac gcgatcttat gaagtccgtc
    10261 agaggacaga tagtagcttt tgaaatgagt acaattcata gaaatttacc tccttggggt
    10321 taggatttcg tacaatttta cttatcaata gtatagcact ttttggggga ataatcaagg
    10381 ggaagttttt aataggagcg gagtttaatt gaggagggag gagtgaggaa tgaggaatga
    10441 ggaatgagca tagcagacca aagtaaattg caagcataaa gtaaaataaa gc
//
LOCUS       DAWEMR010000088        10226 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2761_length_10226_cov_10.507816,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000088 DAWEMR010000000
VERSION     DAWEMR010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2761_length_10226_cov_10.507816"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    551..560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2868 a         2458 c         1842 g         3048 t
ORIGIN      
        1 gtgaaagctt atagcctata gttttcgaca tctgagcttt attctcagcc gatacatgag
       61 tgtaagtatc agcagtaagc ttgtatgtac tgtgaccgag ccactccgaa acctctttca
      121 tactgaaacc gctgttaagc ataagcgttg cattgctgtg tctaaggtca tgtatgcgaa
      181 ttttcggcaa atcgttcttg cggagcaagt cttgaaaggc gtgcagtaca taatcatagt
      241 gaagcggtac accctcagcg ttcacgcaca cataattcct tgtcttgccc agcggagctt
      301 gtctgccata aagctttagc aaataatcaa gttgttcatc actaagagga aattcacggc
      361 gagattttac agttttcatt ctattatttt gactttcgac ccaatgccct gacttatagt
      421 cttttatcct cgttcttgtt tcacggatat aaagacaacg cccgaggaag tcaacattct
      481 cccaccgcaa accgagtatc tcagacttgc gaagtccaaa ccacacagcg agatacacaa
      541 agctttctat nnnnnnnnnn taccataagc tacacgaagg agcttcaaga gctgttctta
      601 tgtataatat gacatttcat tttccacctt tcgaggaagt gaaaaagccg tgtaaggatt
      661 tttgcttata aaatcgttct tatatgcgta attcagacac gcacgcatga cttcatgatg
      721 tttacggagc gtattcacag aaagccttgt atcatgcagt atgtgccttt gatagccctc
      781 tatgtgcata ggcttgacat caacaagtct aagtcctttg ctcttgaaat aagggtaaag
      841 gtattttgtt atgataccta catagccatc ataagttgac ggagattttc tgtaacacgt
      901 ttcattattc catactatga tatagtcaca gaacaaaatt ttatccgtgt caatgttttc
      961 aatgctcatt atcattttgc caaggtcctt tcctgatgat agttgttata gatttttatc
     1021 tttgtcacgt tatcaagctg atgaaagacg gttcttgaaa gtctgtgctt gcggagatat
     1081 tcaagaaaac tttttgattc agttgcaggc gaagtgctgc gtaaagccct aacaatatca
     1141 gaattgcaat cgttattata aaagctttca aggatttgtt gctgaacgtt ttcagagata
     1201 gaaagataag cattataact aatcctgcaa gtatcagata gaaaacgttg aaaaacaata
     1261 agcatttcat cattcattta gttcactcct ttcaaaataa tcatcatatt ccttgcgata
     1321 ctcatcagag taaatataat caaggaaatc tgctatatta tcaaacctag atgaaacctc
     1381 ttcaaagttc ggtatgatat tcacatttgt attgtactta tactggttag aggtataagg
     1441 ttttgtaata gccgactttg aaacgctatc aaaatcggta ttactgtata tgatctgagg
     1501 gtcacgatta cagtttcgac tgctccaata atacttgccg aatatcttat tattgccctt
     1561 tgttatatat tttgtaatat agaacgcaag agccgccgaa ttattctcca caggaatagc
     1621 cgtggaaaaa ccgtatttcc attcagggat attatataca acgtttctaa cgtgcaagtt
     1681 cttttcctct atggtcttta atgtcacagg tttgttatat ccagttacga gccttgtgcc
     1741 tgaatcgacc atatcaaagc aatcattgat aagagcgtga caatgtatac cgccgttctt
     1801 atgcctttca ggaatgagca agtatttcat atctttccgc ttgacctgat tttcaagcca
     1861 ccgcctaagt tttttcttaa caaaatcagc attggaaaaa tcgtactcac tgccattaaa
     1921 agtgatagtg agaaaatacg cccactcatt tgaaaaggct atatcaaaga ccttgtcttt
     1981 tgcacgcttt aatatatcag tccgttcccc tctttcctct tttgaaacct ttgcaggttt
     2041 tttgattata tcaaacatat ctgtttgagt atcttcttcc tgctgagatt tctcaaattt
     2101 ctcccatttt cgtttaagct gtaatatttc ttgattttgc tgatattctt caaggttttt
     2161 gtcaacgaat atgtaattgt tgcaataagt tgttgtcgaa gagccgtcag cgtagatttt
     2221 tgttttagta ttttttaaaa cgacctcagg gggtaaatca taaaaatttg ccattttccc
     2281 accgccattt tagtttttga cggaaatttg cggttattat caagtatata accgcaaatt
     2341 tctaagcttg caagctgttc gccacggcgc acgcaggagc gtgcgcacgt ggctgaatca
     2401 atcttgcata gcttttaaaa ttctgcttgc tattttctct tgctcacttg tgcgcccgat
     2461 tttcagccca ttaacgattt cttctgtatc ataaagcgac cttaattcat cagtagcaca
     2521 gaaagtttct ttccattctt tcggacgttt cctcgttcct gcactgccct gctctccgtt
     2581 aatgagatag ttttcttttg tatagcactt attgacgata agacgtgaat taaaatacgc
     2641 cttacaatct atgatataat tgacctgctc acgaattatt tttgtacacc ttttccactc
     2701 ctgagccgac ccccatatac acttgtgtaa atgccgttgc agtgagatat attcgagaag
     2761 ctcgtccgga gcatctttcc atgattgaga attaagagtc aggtgcattt catcgaacag
     2821 aaacagcacg ccttgattaa caccgttttc gtcaatattc tcaacgttca agatatcttc
     2881 ccaacaatca aaaaatctgt cagccacttc cgtgtgaaaa tttgcacaaa taagcacttt
     2941 cggaaatcta ctcttgacct cttgcgcacg tttcaccatg cttatagttt tacctcgacc
     3001 gcctaagccg ttatagagat atagcccata catattgaac ggaacttctt cacctttaag
     3061 ccgctttcta atagtcttga aagtgtcctt taccgataga gggaacgcat gaagcacagg
     3121 agttccaaac agcataagaa gcacgataac acccaccaca acgcttccca aggcgagagg
     3181 tataagcata gctttccaat tgatattagc aaatgccgac cacattataa aagccccctt
     3241 acaaagttca caagtgcaga tacaagtaga agtccgagaa caaagaaaat gctctcaaac
     3301 atcaattcaa gatttaagaa ttggtcaagc tgatacagaa aagagatcat atccctaaga
     3361 gccgaataag cttcatcact tattgagaat gacttaaaga acggcagact aaagaacagc
     3421 tctactattt tcgcagttat cattattctc cctcactttc acttgattca tgaagctgta
     3481 ttccgaagca acggaacaaa gccttaattg tcgcatagat acagatagcg tacattgcta
     3541 tagttgaagc attgaacagc gcactcttaa gctcgttcgg agcggagttc atattaaaat
     3601 caaagtcctt tccgaaaagt gtaaacgtaa ctgaatttga tgatgattgc ttaccctgct
     3661 gaaaagcttt tctcaacttt gcataagcag gaaacttgct ttctatagcc acattcaaat
     3721 cttttgagtt aggtacaaaa agataggtca cgagcttctt caagtcgacc acgagattat
     3781 acagtgcaat gccgatattt ttaacaatag tccacaaaca cttgccgagc cactcaaaaa
     3841 tgcctaagaa gttgaagaat acaaatttca aagccgccca cagccaacgg aagaagcctg
     3901 taaaagcatt ccaaagaaat tcaacaacag cttttacaaa gtctgctatg ccgtccaagt
     3961 catgaaacat atcaaaatta acgtaatccc ttatatcagg aaaatcagta tctatataat
     4021 cagacaatga cggaaattct tcatagtctt tcttttcgct gaaaggctct ttcttgtgac
     4081 tatctacagt atcaacaaga ctgtactcat aacttgcggc gcaaaatcta tccttatata
     4141 gcgcttcatc acccttgccc ttagccgcta tcaagaagaa ataaagcttg cccgtatttt
     4201 caatatcttt gttactgtta taccgcataa tgccgtcacg cataacattc agaggaattg
     4261 agccatgcaa agggttttct ttcgtgaaat ctcccgaagt gtccataggg agatagtacc
     4321 acccgtcaga gttaggataa tcccattctg attgattaga cacggcaatg tttacgttgt
     4381 atacttcatc actttttcta ggctcaaaat caaacaaaaa atttttgcta tcatcatcat
     4441 aagtaataga agctatgtat ggctgactac caggataatt caacgaagtg ccgttattac
     4501 tgatatcaat atttgtatga acaacatgaa attgcccccc agtgttgcca gttgcattat
     4561 agaaagacat attggaatat gacgcgttaa ttccagaacg ttctatatac attttaccag
     4621 tattcatgtt ataattaacc tgacaataaa atcttgcgtc aaagctttta gaaacaaaat
     4681 tttcaaggtc taaactatca gtatttaaaa cccataggaa ataacgtttt tcattccatt
     4741 gtgtatcagt gcaaaaaata taatttacat tatcagtatc acagccaagt gatttaagtt
     4801 tatcgcaagc catgttatac gcattagtct gaatagcaat ttcaccgcca acacccccac
     4861 cgcctacatc atcaagtgca aatacaggca caacacaagc cgaacacatc acgataaggg
     4921 caagcactaa tgacagcgtt gctttaagtt ttctatttat cataattccc ccttaaaaat
     4981 tggcataata aaagggcagt tcactgaatg aactgccctc gttgctgtca ggcttacgcc
     5041 tttacgtact ttttgaacat tctgatagca atgccgatta cagttgtcag agttatcaca
     5101 gggataagag cgacgataga atcggaaatg ccctgaatag cagagttagc gaactgtgtc
     5161 ataacttcac cgacatttac gagagtattg ccaccttctg cagttgtaga aacaggattc
     5221 attaacacat tctcctttct taattaatta agctatatat ccacttgcca aacttgatga
     5281 caagataaat accgatagat attgttatca aaaagcatat agtgcctaaa tatgaaattg
     5341 taatattttg attattgatt atagtgtgct gattttcgat aacagccgac atagtatatt
     5401 catcagtctg ctcagaggta gaaacagacg atacatcaat ttgtgaagaa gtgacatcat
     5461 tcaacgccca caacctcaat tccctgagcc tgccgctcca gttccttaac acggaactgc
     5521 agtttagaaa tttctttatt tttcttatca attgccttaa agcaacgagt aaggcaataa
     5581 aacagggcaa gcgccaccac caagcaaaaa taaagtgcgt atactgtcat gttcaagccc
     5641 ccttagataa tgaccgcttc aagcttcttc ttatcgttgt agaaatactg gatttccgtt
     5701 ccgacaagtt ctccgatatc tttcatagac acatctttgc cgaacacgtt tcctctttcg
     5761 ctccaagcac acttgcagtc attggcgata gtgtaaccga caccctgaac gaaatttgaa
     5821 tcatctgcca acttgttttc tataggcttt tgcacctgta gcaccaagtt gtcatagtcg
     5881 attgattttc cgttatcgtc cgtaaatgtg cctttcttgt ggattgctcc tataagtatt
     5941 cctctcatgt tttttccttt ctgcggttga ggttatccgc tcacctttac tttgcgttta
     6001 ggtcatatgt aacctacaat tagcattata ggtcacatat agcctattgt caagactttt
     6061 aaggtcactt gtgatataat ttgtttactt aaacaaaatt aaggtaatta aattatgata
     6121 aataaccaaa agtgtaacaa caaaggagtg acaaaaatta gtacaaatta ttatccaagg
     6181 ttgaaagacc tcagagagga caaagattta agccaagaag atgtggcaag aatactaggg
     6241 acagagcaat catattatgc caagtatgaa aacggcgtaa gaccaatccc atttgaaaga
     6301 gtaataaagc ttgcgatttt ttatgatgtg agcattgact atatagcagg attgacaaat
     6361 gacaagcgcg gcagaggata caaggagcaa agcaacggaa aatacaacat aacacagaaa
     6421 aacagcccta aggctgttat caaaatcaag gaggaaaagt aaatggaagc ggtattaaca
     6481 accttaacat tttggtttat agccgggcta atcgtattta tactgataat catatcaatc
     6541 attggaacat ggtttgaagc acgtgaaatg cgcaaggaac tggagcaggt caacgcatac
     6601 cttgcaacgc tcaatgacaa cttgattata ggttttcaga acaacgaccg ccaaagccgc
     6661 aacttctgag agcctgccgc cctcgttcct gctttcctga gctgtcgctc ttgccgtcct
     6721 gcggagcttg tgcgcttttc tgcactgtgc tgtcgcccct gccgtgctgt ttctccctcg
     6781 tggagtttgt gcgctccccc tgcgctgtgc tgtcgcccct gccgtgctgt ttctccctcg
     6841 tggagtttgt gcgctcctcc tgcgctgtgc tgtcgcccct gccgtgctgt ttcacccttg
     6901 tggagcttgt gcgctccccc tgcgctgtgc tgtcgcccct gccgtgctgt ttctcccttg
     6961 tggaacttgt gcgctcccct gcgctgtgtt tggtgcgaac tgcgttcgca atgagggggt
     7021 atcttgagcg gcgttcccct ctttttggag cattgaaagc attgaaaaca ttgagagtgt
     7081 tgaaaaatca tagattttcc aacactccca acatttccaa cacttccaac accccaaaaa
     7141 ttcaccccct agccgctcgc atgatagatt tcacagatat atcgctcagc tctttttctt
     7201 ttctttgaat gtgcgttgct cttgcgaggg gcttcactca tgccgttcac cgcttgtcag
     7261 ttctcataac ccgaaggtcg ttggttcaaa tccagctccc gcaaccaatt tcaggactat
     7321 cgtttgatag tccttttttc atgaccaaat cacccacaaa taccacaaaa agatcggtca
     7381 gtaatcccat ttgggatcta ctgaccgatc ttgctgttta ttcacttgca caaaacataa
     7441 cgtcagcctt aaatcaacgt tccttgtccg tcagctcaaa cctgatactg tgcaggtcaa
     7501 gcccattctg cgcaaagtat atcctgaaag ttgtgaaacg tgagaacata ggcgtttcct
     7561 tttccattga aacagccttg ccaccagtgc cgttgaacgt gaacgtaccc accgcagtac
     7621 ccatgctgaa cagcgtcata ggtatctgcg caagctcact ctgtgtagag cttgccgtaa
     7681 ctatcaccct gtaaataccg aagtttgaca ggtcaagagc aaagctataa gaagtgcctt
     7741 tctttgtttc aacatcacta aggtcaagag ttatgtcacg gcctacctta taaaactgta
     7801 caggcttgtc gtcctcctgc tcctcgtcct ctcggtttat caccttgaca gtatctccta
     7861 taccctccgc acgcttaaga gcattagtgt gaagaagaaa tccgcatatg ttcgccgcat
     7921 ttctctgaag ctcacacctc tctatgccgc cattttcaag ggcaacaagc gtgttatcgt
     7981 cattcttttc tccgtcagga caaaccatgt aaacatcgtt ctgcgcccta gccatagctg
     8041 caaaatccgt cttgtcaggc tcttttccac gaacgtttat gttcgcccac cagtccgtca
     8101 tggtgaagcc cttgaagccc cattcctcac gaagtatcac tgtgttaagg tcaaagtttc
     8161 ctgccgtcca caagccgttt actctgccgt atgtggtcat aacagaggaa gctcctccct
     8221 cttttaccgc tatctcaaaa ccctttagat atatctccct gagtgctctt tccgatacca
     8281 ccgagtcgat aaaatgcctg tttgtctcac ggttgttcgc acagaagtgc tttatcgtac
     8341 ctgtcacccc tgcaccagcc atgcccttta actcagccgc cgccattttg cccgtaagaa
     8401 aaggatcttc cgagaaatac tcaaagtttc tgccgttcaa agggtgtctg tgaatgttca
     8461 ttccagggcc aagcagacaa tccaccttgt tagctgccat ttcaagcccc ataaaagtga
     8521 aaagctcact tgtaagctcc ttgttgaacg aagaagctat catagtgccg tttggcaggc
     8581 tgaaagcctt tgtgccacag tcaagtctca tgcccgacgg gccgtccgaa cagcagccag
     8641 caggtatgcc aaagccattg agattttccg acactccccc gaacgccgat gccgtaccag
     8701 ccgtgaccct agggcttccc atgccctcgc cccttataat gcaggaaagg tcatagtctg
     8761 aaagctgtgc aataaattcg tccattgtgt tcttgccgtt cttaacgtcc caaagcttta
     8821 tgcccttgtc accagtgtat gctatctctt tcggaagctt ctccaaacgc cttgcacttt
     8881 cgtctacctc agatacaggc acagcctctc tgccaatgct gaaagctcct ccctcgcaaa
     8941 caggctttat cctctcaaaa ctctccacag gagcaagcga ctgagtgagc ctttctgtta
     9001 ccagatcctc accctgctca aaactgcaag catattccgc acttctcacg tcactgccca
     9061 catagaattt atattcccct gcttcaagca catagcatga cttgttgccc gtcacacccg
     9121 aatcgtcata agaagcaagc tttgcgatat ccactgctat ctcaaccatc tgctcttcct
     9181 gtggtacaag ctccttagtc ttttcaaaac cgcaaagcac tctcgccgcc ttgccgagcc
     9241 ttccctgcgg agcttcgcag tagacctcta ttacctcttt gcccttgtaa gaaccgatat
     9301 ttttcaccga tacagaaagc ttcacagccc cctctgttat ctcagccttt tccgtcttta
     9361 tctcaaacgc agtgtatgaa agaccaaacc cgaacggata aagcacattt tctttagcaa
     9421 aagtctcaaa atagcgatat cccacataaa tatcctcagc gtaaatatcc ctgtttttct
     9481 ccctgccgaa attagcgtcc gaagggtagt ccgacgcctt gtatgcgata gtgtcaggaa
     9541 gctttccgct tgggctgacc tttcctgtca aaacgtccgc tgtacccttt ccgcctgtca
     9601 ttcccccctg ccaaacataa agcacagcgt caggggcaat tctgtttatg tcggtcatat
     9661 cgatgatatt tcccacattg agaagcaaaa caactttctt gaaattcttc ctcaccaccg
     9721 tgagcatagc tatctcgtca tcgcttagca aataagagcc tgcctccagc ctgttgtcct
     9781 gctcctcgcc tgccgtcctg cctattatca caatggcagt ttcacagctt tttgcagttt
     9841 ctttgacaag cccctcgtca agtggcatct ctttctgcga ccaaggctct ccgccccagc
     9901 cctcgcctaa gtcgaatggg ttttccttgt cccattttct gtatgtgtca agaagcttct
     9961 cattgacctt tacaccgtta tcaataagcc cgtccagaat gttcacgacc ttagtaacgt
    10021 tcaccattcc tcccgagcct gttccgcttt tataataatg aaactgtatg cgtccgaaaa
    10081 cagccacctc tttgtctgta tcaagtggca gagcattatt ttcatttttg agcataacga
    10141 ttccctcaga aaccattttc gccgcagtat caagatattt gttccaatca agtattcttt
    10201 ccatagcttt cacctttcca aatttt
//
LOCUS       DAWEMR010000089        10213 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2769_length_10213_cov_11.928923,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000089 DAWEMR010000000
VERSION     DAWEMR010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2769_length_10213_cov_11.928923"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3146 a         2048 c         2550 g         2469 t
ORIGIN      
        1 agaacaaaaa tgaaaggttg tgatttttct atgggcatct atacagagaa aagagcgcag
       61 ctcatcgaga agatcaaagg tttggaaaag cgtatcgcaa gcgatactgc gaaaaagaaa
      121 acgctggggg acaagctgaa agaagtttcc cgtctggcta tggaagaaga atacaactgc
      181 gagccccgtg agcttgatga gatcatcacc tcggagcatt cgctgttgca gaaactccgt
      241 gcaagcggtc tgtccgatga agatcttctg aaactggtcg gtggcggaaa tgctgaaaat
      301 gagaaaaccg ataccgccga tcttcctgct gacttcggtc agcaaatgag ctttacggat
      361 aaagcgaatc cctatgagga ttaaaactat ccggtaacaa aaaatgtagc cgtaaaatac
      421 tctgtcggct caaaacactg agccgacctt acggctatta tgagggtgag agatagaaag
      481 cgaagccctc gactctaaag catagagaca gagaagctaa aggagtaaaa ttcgctatga
      541 ataaaaaagt tactgccgta gagaaagaaa aggcagagac aacttttaac gcaacgtccg
      601 agtacagaat cggacatacc acttacacgg tgacaaccgt gttcaaccct gcttcaaagg
      661 agagcctttc agatattctg aaaaggctta ttatccggga gagtgaaata ctcctcggtg
      721 aatccggaca agaacccgag aagcaggctg tgtaactttg tgatatcggc gcattgtatt
      781 tattgtcggt gcgcgctata atgataacat agcttgctgt atctgtcgga aagagaggta
      841 aattatgaca gacaagatca cagcattata ttgtcgcctt tcgcaggacg atatgttaca
      901 gggtgagagc aacagcatca ccaaccagaa ggctatcctc aagaagtacg cagaggataa
      961 tggtttcagc aatcccgtgt tttatgttga tgacggagtt tcgggaacga cgtgggagcg
     1021 tgagggcttc aaagcgatgc tggctgacat tgaggagggc aaagtcggta cggtcattac
     1081 taaagacctt tcaagacttg gacgtgatta tctcaaaaca ggcgagtaca tcgagataat
     1141 ctttccggat catgatgttc gttatatcgc tatcaatgac ggcgtggata ccttaaagtc
     1201 tgaaaatgag ctgatggctt tcaagaacat ttttaatgac tggtacgccc gtgatacgag
     1261 caagaagatc agggctgttt tcaaggcaaa ggggcagtcg ggtaaacatc tttccaatcc
     1321 aatctacggc tacaagcact cggaaacgga taagaactta tgggtgatcg acgatgaagc
     1381 cgcagaggtc gttcgcaaga tttttcatct ttgcattgac ggctatggtc cgacgcagat
     1441 tgctcgcata ttaacggaac aaggcatccc cacgccaact gcctacgcat tgtcgcaggg
     1501 cagggataat ggtcataaga atgccaagct gcaccgttgg ggcaatgaga caattgccca
     1561 tattctcgaa aaggctgaat actgcggaca cacagtcaat ttccgcactc atgtgaagtc
     1621 ctacaaaaac aagaaacgtg tggataaccc gaaagaggat tggctaatct ttgagaatac
     1681 ccatgaagcc attatcactc agcaagagtt tgacttggtg caagagcttc gcaagaacaa
     1741 acgccgtccg acaaagcacg aagaagtcaa tccgttttca ggcatctgtt actgtgccga
     1801 ctgtggaaag aagttgtatc tgtgccgagc gacaaccatg actgccgacc aagagcatct
     1861 gaaatgtggc acctatgcaa aagacaagaa cggctgtaca attcacttcg tcaggacaat
     1921 agtgctgaaa gagatcatac tcggtgaact aaataagatg gttgcctttg tcaaggataa
     1981 cgaggacgag tttgtgcaga tggctatgga taattccgtt cagaagcagt cggcaacgaa
     2041 accgatgaaa acggtgaaca ggtgcagatg gctatggata attccgttca gaagcagtcc
     2101 tcggagcttt ccaagtccag gaagaagctg aaagaatcgg agaagcgcat cgcagaactg
     2161 gacagactgt tcacaaggct ttatgaggat aacgtatcgg gcaagatttc cgatgagcga
     2221 ttttccataa tgtcggcagg atatgaggac gagcagaaga aactgagagc aaccgtagca
     2281 gagcttactg acttcatcga aacggcagag cagaagtcag ccgatgtgac cgcattcatc
     2341 agcgtggtgc agaagtatga gcgtatcacg gagcttactc ctgagatcat gcatgaactt
     2401 attgagaaga tcgttgttca tgctcccgat aagagcagcg gtcaccgcac acagcagatc
     2461 gacatttatt atcgcttcga tgtcgcagta tcaactgcgg tcgctgacag tatgaagtac
     2521 gacaaaaaga gaaaggttgc gtaaccgcca tggttacgca acctcatctt caaatcataa
     2581 aaacttcttt acgagtaccc gttttcgtgg tgcggtgatt ttttttgcat aagccgtcct
     2641 ttgtccgcat aacttgtaca aaggacaaaa actaggaggg cggatatatg ttcagaagat
     2701 tgcagaatat tttgtgctgt gtggtgatag caggctttgc ggcagtgtgc tggtatggca
     2761 aaaacaacgg cgaagccgtc atgacaggtg ggacggtgaa aacgaccatg gataagcctg
     2821 tggtggtcat cgacccaggt cacggtggta tggacggagg ctgtgtatcc gttgacggca
     2881 cgcctgaaaa ggggataaac cttgcggtgg cggaaagcct ccgtgacggt ctgaagcttc
     2941 ttggctatga tgtcgtttgc acccgtgaaa gcgatatctc tatctatgac aaaggcacag
     3001 aggggcttgg ggagcagaaa aagtccgata tgaaaaaccg ccttgctata ttctcgaaat
     3061 attccgaggg catatcagtt tccatacacc aaaatcagtt taccgacagc cgctacagcg
     3121 gtgcgcagat gttctattct aaagacaatg ccgatggtga gaagcttgcg ggagtgatgc
     3181 aaaagcagtt cgtttccctt ttacagcctg acaataccag agaaactaag ccggtaggcg
     3241 acgagcttta tctacttaat aataccaaaa atcctgcggt tatgatagag tgcggttttc
     3301 tttcaaaccc tgaggaggct cagaaattgg agaatgagga ctatcagcgt caggtggcgt
     3361 ttacagtgct gacaggtatc agcgaataca ggctaatgca gaaatagtcc attggtgcgg
     3421 cggcagtgag aaaacactgt ccgtcgtttt ttgtttgtaa aacgtcacat aaattgcttg
     3481 acttttaaaa ggggatattg tataatataa tagtgtattt atagcatagt atatttttta
     3541 actttttcct gtcataaatt ctgacaggag aataaataaa ggtggattga aacatgggat
     3601 tatttgacaa aatattcggc aactattcta aaaaagagct tgccaagatc gagcctataa
     3661 agaataaggt actcgagctt gaaagtaagt atgccgatat gtctgacgag gagctgggcg
     3721 gacagactgc cattctgagg ggcaagctgg acgagctttc aactcttgat gacgttcttc
     3781 ctgacgctct tgcggtttgc cgtgaagctg cgttcagagt gcttggcaaa aagccttacc
     3841 ctgttcagat aatcggtgct atcgttcttc atcagggacg tatcgccgaa atgaaaacag
     3901 gtgaaggtaa aactctcgtt gcttgtctgg cggcttatgc taactccctc acaggaaatg
     3961 gcgttcacgt tgtaacagta aacgactatc ttgctaagtt ccagtctgag gaaatgggca
     4021 aggtgttcca cttcctgaac acttctatcg gcgttgtgct tacgggcatg aacaaggagc
     4081 agaagaggga agcttataac gctgatatta catatggtac aaataacgag ttcggatttg
     4141 attatctccg tgacaacatg gttatctata agaaagataa ggttcagaga gagcacgctt
     4201 tcgctatcgt ggacgaggtg gactctatcc tcatagacga agcaagaact ccgcttatca
     4261 tttcaggtca gggcgacaag tcaacaaagc tttattcact tgccgacaga tttgcaaaga
     4321 ccctgaagcc tgttacagtt gttgaaatgg acgataaggc agataacgac cttctggacg
     4381 gcgactatat catcgacgaa aaggctaaga ccgcaactct tacaaagagc ggtgttaaga
     4441 aagcagaaaa ggcgttcaac gttataaacc ttatggacgc tgataatatg acccttgccc
     4501 accacatcaa tcaggcaatc aaggcaaacg gcgttatgaa aaaggatatc gactacgttg
     4561 ttaaggacgg agaagttctt atcgtagacg agttcacagg ccgtatcatg cagggcagac
     4621 gtttcaatga cggtctgcat caggctatcg aagcaaaaga gggcgttgag atagcaagag
     4681 agtcaaagac tatcgctact atcacatatc agaactactt ccgtctgtat ggcaagctgt
     4741 caggtatgac aggtactgca cttactgagg aggacgagtt cagagagatc tataagctcg
     4801 acgttatcga gataccgaca aataagcctg ttatcagaat tgaccaccca gacgttgttt
     4861 tcaaaacaga aaagggcaag ttcaatgctg ttatcgagca ggtgatagag tgccataaaa
     4921 agggacagcc ggtactcgtt ggtactgttt ccgttgagaa gtcagagtat ctttcaagac
     4981 tgctgaaaaa taagggcatc aagcatgaag tgcttaacgc caagcaccat gaccgtgagg
     5041 ctatgatagt tgcacaggca ggtaaatacg gtgctgttac tatcgctacc aacatggcag
     5101 gacgtggtac tgatatcatg cttggcggta atgctgagta taagtccctt gcagacctcc
     5161 agaaaatggg ctattctgaa gaagtggctg tcgaggcagc aggcttctca aatactcagg
     5221 acgaggaagt tctggctgca agagcagagt ataagaagct ctatgcaaag tattcagaag
     5281 aggtaaagga gcttgcagaa aaggttagag aagcaggcgg actttatatc atcggtacag
     5341 aaagacacga gtcaagacgt atcgacaatc agctcagagg acgttcaggc cgtcagggcg
     5401 acccgggcga aagcacattc ttcctctcac ttgaagacga ccttatgaga attttcggcg
     5461 gcgagagaat aacagccatg atggacactc tcaaggtgga cgagaatact ccaatacagt
     5521 caaaaatgct cacaggcgtt atcgagtcct cacagaagaa gatagagggc agaaacttca
     5581 acatcagaaa gaacgtcctt aactacgacg acgttatgaa cacacagcgt gagataatct
     5641 ataagcaaag acagcaggtg cttgacggag aggatctcca tgaaaatatc ctcggcatga
     5701 taaaggagtt tgttgaggat tctgtaaata tgtatatcac cgacgaggac gttcaggata
     5761 actggaactt gcagggtctt aaggacagac ttatgggcat aatcacagtt gaggacgact
     5821 tcaactatac cccacaggaa cttgacgaga taacaaagaa ggatatcgtt gacctccttg
     5881 aagacagagc tttgaagata tacgcaaagc gtgaggaaga cttgggtcag gaacttcttc
     5941 atgagataga gagagtcgtt ttgcttaaag ttgtggatac aaagtggatg gcacatatcg
     6001 acgatatgga cgagctgaaa aagggtatcg gtctgcgttc atacggtcag aagaacccag
     6061 tagtcgagta tcgtatggaa ggtatggata tgttcgacga tatggtagct tctatccgtg
     6121 aggacactgt aagaatgttg ttcaccattc aggtaaggaa gaacagcgcc gctccaaaga
     6181 gagaggacgt tctcaagccg ggcgagcatc ataacatgac cgtaagaaaa gctaagaagc
     6241 cgggcaggaa cgatctttgc ccatgcggaa gcggcaagaa gtataagaac tgctgtggtg
     6301 ccggaaacgg tgtacaggct gacgaagccg agcaggcaga gaatagctct gaggaataaa
     6361 aacaatatgc aggacggtga aaacagccgt cctgttttgg atataagaat aataggagtg
     6421 tagtgaaatg tcaacagcat ttaaaagcgc agatatactt ctgcctaaga acgccgatat
     6481 gtcaaaatgg gcagtagttg cctgcgacca gtatacctct gagcctgagt attgggctga
     6541 cgtggaaaag ataacagagg gtgcaaaaag cgcacttaac cttatcctgc ctgaggtcta
     6601 tctggaggac gataacgttg atgaaagaat agccgacata cataagaata tggacagcta
     6661 tatcagcgag ggcgtttttg aagagtataa ggacgctatg atatacgttg aaagaacaca
     6721 gtctgacgga aaggtcagag cgggcatcgt tggcgctatc gaccttgaag agtatgacta
     6781 ccgcaaaggt tcgaagtctg ccgtaagagc aacagaagcc acagttgtgg agagaatacc
     6841 gcctagaata aaggtaagac gtggcgcacc tgttgagctt ccgcatatca tgatactcgt
     6901 tgacgataca gaaaaaagcg ttgttgagcc gcttgaagtc cacaagggag aaatgaaaaa
     6961 gctctatgac tttgacctta tgaaaaaggg tggtcatatc gcaggctatc ttattgaaaa
     7021 gcctatgcag gaaaagataa tagccgcact tgaaaagctt ggtgatatcg acgctttcaa
     7081 tacaaagtat ggtcttaagg aaacttcacc gctcgtttac gctatgggtg acggcaacca
     7141 ttctcttgct actgcaaagg aattttacga ggagcagaag agagagaacc ctgataagga
     7201 catgagcaat gcactttgca gatatgccct tgtggagata gtgaatctcc attcgcctgc
     7261 acttgaattt gaagctatcc ataggatagt tacagacgtt gatacaaagg ctcttatgag
     7321 cgaaatgaca gcggctcttg agctttctga ggaaaagtcg gagcaggcaa tagttgtctg
     7381 cgacaatggc gaggaaaaga ctctctatat acataagcca acttcaaagc tcactgtcgg
     7441 ctcattgcag aatttccttg acagctatat caaggaaaag ggcggcaagg tggactatat
     7501 ccacggtgca gaggtaataa gagagctttc cgcaaaggaa aattccatag gctttatgct
     7561 ccctgatatg ggcaaggaag agcttttccc tacagttata gttgacggcg cactgccaag
     7621 aaaaacgttc tccatgggtc atgccgagga taagagattt tatgttgagg caaggaaaat
     7681 attgaaatag aaaaattaaa gaaatgttga aatagaaaaa tcgtagacaa tatattttaa
     7741 aaggagtttt tacgatgata attgaagaag tcaaaaagga acttacagga catatcattc
     7801 ccttctggaa caagctgaga gatgatgaga acggtggatt ttacggcttt atgagctatg
     7861 acctcaagct ggataagcag gctgataagg gcgttatcct ccacgcaaga atactttggt
     7921 tctattcaaa agcgtatatc actctgggcg ataagacact tcttgacaac gcacgccacg
     7981 cttacgagtt tatcaaaaac cactgcatcg actatgagta cggcggagtt tattggatga
     8041 tgaactataa gggcgagcct gccgacacta tgaagcatac atacaacatc gcatttgcta
     8101 tctatgcact gtcatgctac tataacgcaa gcggggataa ggaagctctt gagcttgcct
     8161 ataagctttt ccacgacatc gagaacaaca ctctcgacga gtacggctac agagaagctt
     8221 tcgacagaca gtggaacctg gtatcaaatg acgctctttc tgagaatggc ttgcatgctg
     8281 ataagacaat gaacgctatc ctccacctta tcgaagctta taccgagctt tataaggcag
     8341 accacaacga ggaagttgct aaaaggctta aattccagct tggtcagatg agagatattg
     8401 tttatactcc tgaaactaac gctctgaaag ttttctttga cacaaagttc gaccttgtgg
     8461 gagatattca ttcattcggt cacgatatcg aagcaacatg gcttatggat cttgcctgcg
     8521 atactcttgg ggacgaggag cttaaaaagc agtttgctga aatggatctt aaaatctctc
     8581 ataatattca gaatatcgct ctggataacg gcgcactgaa caacgagcgt gagaacgata
     8641 agatagacaa gaagcgtgta tggtgggtag aagccgagtc tgttgttggc ttcatcaacg
     8701 cttatcagca cagcggagaa aagaagttcc ttgaaagtgc aagaagcgtt tgggagtata
     8761 tcaaggctga gataatcgac aagagagaag gctcagagtg gtatgccgag gtttcctatg
     8821 accataagcc acatgattgg aaagagatag taggtccatg gaaatgccct tatcataacg
     8881 gcagaatgtg catggaagtc atcaacagag gcgtggactt ctgatgttca ggtatgaaac
     8941 acacctgcat accgccgagg gaagcgcctg tgcctctgca agcggagcag agcaggcaag
     9001 gcgttataaa gcccttggct atgacgggat catcgtcacc gatcactttt tcaacggcaa
     9061 ctgtgctgtc accaactttg acagttggga ggacagagtg aacaagttct gcaagggcta
     9121 tgagaatgcc aaagaagtgg gtgacgagat aggtctgaaa gtttttttcg gtctggagta
     9181 ttcctatttc ggtgcagacc ttttgaccta tggcatcgac aaggactttc ttcttgccaa
     9241 tgataacatc agtgatataa gcttttatca gtttgccgac agagtgcata aagcaggggg
     9301 cgtgttagtc catgcccacc cttttcgtga agcggattgg tatatccatg aaataaagct
     9361 cctgccgaag tggatagacg gcgttgaggt ctataacagc ggcaacggca aagaggtcta
     9421 taatcagcgt gcaaagtggt atgccgaaca gttcggcttc aagcaaacag gcggtacgga
     9481 caatcaccac ctgtgggtgg aggacagccg tatcagcggt gttgcaactg acgagcctat
     9541 caattccata gaagattaca tagaatttct ccgtgacgga aagcttaccc cgataattcc
     9601 ggaaaagtcg gctgaaacat aaaacataaa taaaaacttc aatgacgttt tttcgccatt
     9661 gaagtttttt tactatgaaa ttttgctttc atttaccgaa acatcgtcgg aataagattc
     9721 cgattcagcg gcagaatcgt cgggaacacg aatgtaagta tctacggcga cgaatgtgtt
     9781 ttcatcaaca taattaaatg ctaaagcgaa agcattttca tgttttccat agtcaaattc
     9841 aattagtatc tgatctcttg cttcgtgata ggttttaata gtatatgttc tggtttcaga
     9901 ccacattttt atcgtatcct gctttgcggt ctcgtcataa aacgtaaaca gcttattgca
     9961 aagatattcc gaatctccgc tgtatttcag agtgttgttt tctcctatta taaaataatc
    10021 tttgcttgta tctccgtttg gataatattt tcccatattc agcgttggtt cctgataggt
    10081 atcgtcatgg ggacagctgt agttaaaagt atttattccg ctttcatcat cttcaggttc
    10141 atcagaagaa actatgttct ttatgccaaa ataccaaaaa caaaatataa atgcaacggc
    10201 aacaattacc cat
//
LOCUS       DAWEMR010000090         9910 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2866_length_9910_cov_14.448909, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000090 DAWEMR010000000
VERSION     DAWEMR010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2866_length_9910_cov_14.448909"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7894..7903
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2576 a         2718 c         1883 g         2723 t
ORIGIN      
        1 atttcccgca aagcttatct cagcgtcagg atagtgtttt tttatgatct ccgcaatggc
       61 aagagcagga tttacatgac ctgccgtacc tccgcctgca agtaaaactc tgagcataga
      121 actaatacct ctttactttt ttttgatgca ctgacgtgaa acgttgagca tgatacccat
      181 ttctgcgagc tgtatcatca gtgcagtacc gccgtagctg aaaaacggca gtgaaatacc
      241 cgtgttaggg aaagcgttgc aggctaccat aatgttgaaa agtgcctgcg agcctatctg
      301 tattgttata ccagccgcaa gaagcatacc aaaacgatcc ttgcttcttg aagcgatcat
      361 gaagccctcg attataaaaa gcacaaacag cagtaccact accattgcac ccacgaagcc
      421 aagctcctcg cagataatag cgaaaacgaa gtcgttttgc gactcaggca gatagtattt
      481 ttgccttgac tcgccaaagc ccagtccgaa ccagccgccc gaacctatag acagcaggga
      541 ttcataagtc tgaaaagtcg ttccgctagg gtcggattca ggatttttcc agctctgtat
      601 acgctctgag atgtaagaaa atcccgaccc tgacgccatt ttatatatca gcagcagcat
      661 cccgaaaaca gcacacacgc ctatgaactt ccaaaaagtc tttttcggca taccgcttat
      721 gaacatcata gacacgccta tgatacccac cagcattacc gccgacatat gcctctgtaa
      781 aacaagcacc agtgccaccg cacccattat caccgtaaac ggtataacgc agaatttcca
      841 atccttgaac ttaggataat tcaccgacat tatgtatgcg aaaataataa tgaacgttac
      901 cttcagaagc tccgacggct gaaagctgat cggacctatt tgtatccaac gccttgcacc
      961 ggcaacgtct attccgaaaa aagctgtata aaacgtaaga agatatgtgg cgataaacgc
     1021 aatatatgct atcttagtgt tctgaaagaa gtgataatca agatagctcg tggcaaacat
     1081 gagaagaaga ccaagtccgg cagacttgag ctggctcacc agataataat atccatcgtt
     1141 atattcactc agaccccacg catagcttgc agaaaacatc atcagaagtc cgaatacaag
     1201 caataccatt acaagaatga aaaacggctt gtctatctcc gtaagtataa atctgaatct
     1261 atgttttctt tttgcctgtg ccacaggttg tttaagagga acgtgactat tatacactat
     1321 gcttccgtca cgttcacgtc taggacctct tatagccaaa caaacaacct cccaacaaaa
     1381 tctctatatg aaaaactttc ccgaatatat caaaaacgct atcgccgccg cagcccctgc
     1441 cacagatatc acagcgaata tcccgatcac ggcgtgatcc ccatgaccct ttttctgcaa
     1501 atgctcatga agcgtatagc ccctcatgag cagctttttt cttatcttaa acacgatata
     1561 ctgcaaaagc ccacaagctc cgtcaacgac caccgccgca cctgccagca ggaaaacgaa
     1621 atattctccc gaaagcagca ccgccatagt cataagacca ccaatcgcca gcgcacccga
     1681 ctcgcccaaa taaagcttcg caggactgaa cccccaaaag agaaagcctc ctgccgcacc
     1741 cattgtgcaa acagcaaaaa gctgagcagc gtcagaacct gcccttgccg caagaccact
     1801 tgacaaccct gccgaaaata tcaaagccgt aacggcacac agcccgtcag tgcccgtgtc
     1861 ggtcttaccg cccgcacaat cgtgtatctt caccatgttt atccccactg tcataaacag
     1921 cggaaaaagc ggataaaaaa gcctgccgaa ctgcacatag cccaagtgaa aaggcataag
     1981 cacagccgtc tgcactcctc cgcagtatct aagccccacg cacactccaa gacatataag
     2041 atactccata catatcttgt aaaaaggctt catgcctatg ccaagctttt tctccttaac
     2101 atagtcctcc gaaagcccga tagccgtgag catcaaaact ccgcccaaag cgatgaacac
     2161 acgcctcgaa aactctccgt tctcaaaact cagcaccgcc tgcgagtcat aaaatataac
     2221 tcccgtaaca atgccgaata taacgcagag cattatgaca actccgccga attttggctc
     2281 agagccgtcc tttgcgaacc tgtcgcctat ataaaaatcg aatttacctg tttttatcct
     2341 gcggaaatat ggtatgaaaa ctctgccgag aacatatccc agcaccattt cacaccccag
     2401 tatcaccgcc tgcacgctcc aactcatttt gtcagtgcat tgaacacttc ttcaagcttc
     2461 attcctctgc ttcccttgaa aagcacagcg tcgccctttg caactacctc tttcaaagcc
     2521 gccgcaaggt catctctgct ttcaaagtgc ctgcaacttt cctttgcaaa gccctttttc
     2581 tcagcaccct ctatatagca ctttgagtcc tcgccaaagc agtaaagcag atcagccttt
     2641 gaagccgcaa catactctcc cacgtttctg tgataggtct ttgagttctt gccaagctcc
     2701 agcatatctc caagcacgca aatgcgcttg ccctccgcct gtgcctgtga aagcaccgaa
     2761 aggctcgctt tcattgagtc aggcgcagca ttgtagcagt caactatata tgtaactccg
     2821 tcaatgacgg taacattctg tcttaaaccc tccggtttga actgagcaat gccctttaca
     2881 atatcctcag gaggaatatc cagtgcaagg cccacacaga acgccgcaag agcgttcttt
     2941 acattatgct cgcccaagca gtttatctgg gcggctatct ttccgccctc atagtttata
     3001 gtaaatgtta tgctgttgtc aacagtctta acaccgcttg cataaacatc gcagtccttc
     3061 tttgatacag aatagtaata aacctttctg tcagaatgta actgcacccc tgcaagcaat
     3121 ttatcatcac ggcaaagtat cagcggagcg tcataagccg caccgtcaag tatctcaagc
     3181 ttcgccttga gaatgttttc ctgcgtgccg agattttcaa tatgtgaaat gccgatgttg
     3241 gttataacag ccacgtcagg ctgacatgac attgacattc tcgatatctc gccctgatgt
     3301 gacataccca tttcgataac agccgcctca taactgctgt caagaccgaa aagcgtctgc
     3361 ggcataccta tctcattgtt gtggttgccc tgagttttca gcgtcttgta tttctgcgcc
     3421 acaacaaggg ctatcatatc cttagtggtg gtctttccca cgcttcctgt tacgccaact
     3481 accttaacat cgaatttaga cctgtaatac cctgcaatgt cgagcaatgc ccttgcagtg
     3541 gagtcaacga tcatacatct tgcaccctcc acaggtcttt ccgtgacaac agcctccgca
     3601 ccaagctcca tagcctttgc ggcaaagttg tgtccgtcaa aattctcacc ctttagtgca
     3661 ataaaaagcg agcctggctt tattgttctc gtatcggtgg agatatcgga aatttcaacg
     3721 tcagcaggat agccgtagct tccatgaacc gccgatatta tctcctgtaa gcttaatttt
     3781 tccattttaa ttcttatccg ccaatttctc ggcagtatct cctttccctt ttcttataaa
     3841 tatatcttta tcgcaaaacc gcccacagcc gctttgcata tcacgggcga acactgttca
     3901 ccccctacat ttttctctta tctgccctca gcttcaagct ctttaagagc gtccgcaacg
     3961 acttctctct cgtcaaaatg tatcttcaca ccacctgcaa gtatctgata atcctcatga
     4021 cccttgccgg caagcacgat gatatcgtcc ttttccgcat gatgtatagc ccagtggata
     4081 gcctcacgcc tgtcaacgat agttatgtaa ggagtcgtct tgccctccag acctaccaga
     4141 acatcgttta tgatatcctg cggatcttca ttcctcgggt tgtcggaagt gacgataaga
     4201 aaatcagcat tgtccgccgc agccgcagcc ataaggcttc tctttgaagc gtctctgttt
     4261 ccgccacagc cgaaaaggca ttttaccttt ccgcttgcac tgttctttat agccgaaagc
     4321 acgttcacaa gtgcgtcagg agtgtgagca tagtcgcata taacagtgaa atctctgccc
     4381 gtcggaacta tctccgctct tcctcttacg ccctgcatag tacccaaagc tctgatagtc
     4441 ttgtcaacgt caaatccaag ggtctcacag caggcgataa cagccagcga gtttgaaacg
     4501 ttgaaaagtc cgggcatatt gaatgaaaca ttgtgcttta tctttccgtc agtgaaaaca
     4561 tacttcacac cgcttgctga aagcagtata tcctctgctc tgaaatcagc ctttgtacag
     4621 catgaataag ttttcgcagg acagcttatc tctttgagat accttctgcc gtattcatca
     4681 tcaatgttga tgatagcgtt gtcagatata gtgaaaagta tcttcttggc ctgatagtaa
     4741 ttttccatag taccgtgaac gtcaagatga tcctgcgtca ggttagtgaa aacgcccacc
     4801 ttataatgac agcctgaaag tcttttctgc tccaagccct gcgaggaagc ttccataaca
     4861 acatagtcgc acttttcgtc agccattttt ctgaaaagct cataaaggtc ataaggctca
     4921 ggagtcgttc ttgcagtagg ataaacgtcg tcgcctatct cgttctgaca agtgccgata
     4981 agaccaacct tacagccgaa tgaagaaagc accttcttta taacagttgt gatagtcgtt
     5041 tttccgttag tgcctgttac accgataaga gtaagctcct tttcagggtg accgaaaaat
     5101 gccgcacaca gcagagggaa agcctctctt gtgttctcca cgattatctg acagtcaagc
     5161 cccagatctc tttcgcagac tacaactgta cagccctttt ctatcatgtc cttagcagcg
     5221 tcatgtccgt caaaggtctt tccttttatg cacacgaaca tcgagcctgc cttgacagcc
     5281 tttcttgtgt cgctcgtgac gtcgcttatc tccatatcag gcagagaagt tttcgcaacg
     5341 tcctttatga tatcatatag tttcataatt atcattcctt atcatggttt cttactgtga
     5401 acctaccgag gtcactgcaa atgttactga cacagccgtt cccattggaa cgctctgtcc
     5461 tgctcccacg ctctgatcgg actgtgcaac tgcaccctcc tcctcgtagc ctgagccttc
     5521 tgctgtcaag ttaagaccat acatatcaag cgtagccctt gcctgctcct ttgtaagtcc
     5581 tttaagttca ggcaccgtaa cagtttcttc cttggcgttt gcttctgtgt aaagcacgac
     5641 cgatccgcca tgctctatct ttgcggcaac aggcgtctgc ttaacaacag tgtcgccctc
     5701 gcccttgacc ttgacctcaa gtccaagatc ttcaagtgtc tttttggctt cctcaacagt
     5761 ataatactga acgctaggta ctgatacctg caagtctgca agctcgcttt cgtcatactc
     5821 agggaaaatt cccacataag gcagaacgtc tgaaagcacg ttcacgcata taggcgctgc
     5881 cacctggctt ccgtagaatt tctcgcctgt ggggtggtct accattacga gcattactat
     5941 ctcaggatcg tcagcaggag caaatgcaca gtatgaagaa acatacgtct gacctgttga
     6001 gtcctcgtca attttctgtg aagtacccga cttaccgcct atcctgtatc cctttatata
     6061 gcagttagag ccgggctgac cttcaacaac gccctcgagt atctctctca tagaagcgga
     6121 ggtgtcctca gaaatgacct gacgtcttac aacagtgtcc attttcttta caatgttgcc
     6181 gttctcatca gttatactgt tcactacctg cggtgtaaca agataaccgc cgtttatagc
     6241 cgccgagtaa gctgttatca tctgtatcgg cgttacctta tttgtctgac cgaatgaaga
     6301 tgaagcaagc tgaacagggc ccatgttgct aagcggcata ctgatagaat ttgattccgc
     6361 aggcaggtcg atacctgtca gctcgttgaa accgaacgca ttgtaatact gacagaactt
     6421 ttcagagcca agcttcatgc ctatctgaat gaatgcaggg ttacatgagt ttagcattgc
     6481 ctgggcaaaa tcctgcgaac cgtggtcggt agttgaccag cagtggaaag tcgtgtcctc
     6541 cacctgtatc tgagtgccgc agccaaaagt atcattaaga gtgatagcct tttcttccaa
     6601 agccgaagca cctgttatga ccttgaatac agaacccgga ttgtatattt ctgaaaccgc
     6661 cttgttcttc cactgaagcg accatgcgtc aagctgtttc tgcttgtaat cgtttgaatc
     6721 ttcatcaagc cccgcaagct ccgccgccgt cttttcatcg gagatagccg caggctcgtt
     6781 agggtcataa gggtcagaag tcgccattgc gtatatctga cctgtcttag gattcattat
     6841 gataccgcag gctctgtcag taggctgatt ttcctcaact gcctttgcaa gctctttttc
     6901 aaggatatac tgaatgttgg cgtcgatatt aagattaaga tcgtcgccgt cctgagcgtc
     6961 atagctctgc ttgtatctgt atggtatctc agttccgtcg cttgcctttg cagtgattat
     7021 cctgccgtca attccgctga gataatcgtc atagtaagct tcaagaccat atatgccgtt
     7081 gccctcatag tccgtgtgac caagaacaac agaagcaaga gtgttctgtg gataaagtcg
     7141 tcttgtggta gggtcgcacc ttacgccgtc aatgccaagc tccgtgagct tatcctcgat
     7201 agccgtggaa acggtctttt ccacctcttt tttcagcacg atgtactgag tatccgtctt
     7261 agtgagtttt tctctcacag tgttagtgtc tatctcaaga ttgagtgcca aaaactccac
     7321 aagctcgtca agacattcgt cccctgattt cgttgcgtca agtctttgct gatatgtagc
     7381 cttcttggac gcgtcgtcct ctttggctat atatccttta agctcctcta tcttcttgtc
     7441 gttgtcatca agcttttctt tgagcataag agggtctgca taaacagtat agaccgttgc
     7501 gctctgagaa agcaccgttc cgtttgaatc ataaatagtg cctcttgacg ctgaaacagc
     7561 cgtgctttta agctgctgag cgtttgcaag ggactgccac ttcttgccgt cctttataga
     7621 cactgaaaag atggtcgcaa ccgtatatat aagtatcgcc gccacaggca ggctcagcca
     7681 aatattcaga cgttgtttca ttctggcact tggtctgtcg gtcataaata tctcctctca
     7741 ggacatcacc gcacatacaa aatcttcaat aatttagcat gatacggcaa atatcgtatt
     7801 ttcacacata caaattaata gaacaagaaa gctaagcaca aatatcttgt ccctaacttt
     7861 ccccataatt attttattca ctaagccccg atannnnnnn nnntattcca gaacagagct
     7921 gaaccagtgt tttatcttaa cgaacacatt gtcgtcctct gcctttacca cctttgccac
     7981 cgagtctgac tccacagtaa catactctat ctgagattta tccagctttt taagccccag
     8041 ctcattctca gcatactcct cgacctttga catattggtc ttctgagcaa gctctgactg
     8101 taagcttaca ttctctccct gaagctgagc aagctgttcc tcctgcctgg tctgctccga
     8161 acataggctt gatatttcga ctctgccgta tatcatcgtt ccaagaacaa cagccaccag
     8221 cagacaaatg cctatcacag ccactaccga tatggttttc tgctttagtg cagggttttt
     8281 cttatatttt atggctctct gagcctccgc ctcctgagcg ttgtcatagg cactgtaatc
     8341 aaatgccaag ttatgtacct ttgccattgt ggtaaaacac ctctcacgat ccacgggcaa
     8401 gcccgttatt tacacattcc caatttcaca caaatttcac tcagtccttg atcttctcga
     8461 tcaccctgag cttagcgctg cggcttctgt tattctccgc aagctccttt tcatcagcct
     8521 ctataggctt tctgttcaca agctttcccc taggcgttct tccgcatatg cactgaggaa
     8581 aatcaggcgg acatatacag cccttgcaga agctctgaaa tctctgcttt actaatctgt
     8641 cctccagcga gtggaaagtg attatgcaaa gcctgccgcc cacgttaaga caatcgaatg
     8701 ccttgtcaag agcgatattc aaatgttcaa gctccttatt aacctctatc ctgatagcct
     8761 gaaaagtctt tttgcaaggg tttttctcac gccttgcctt ctgcggaact gcacttttta
     8821 ttatctcggc aagctccata gttgtctcta tcggtttttg ttctctgtat tttactatct
     8881 tagaagcaat gcttcttgaa aacttttctt cgccgtactc gaaaattatc ctcgcaaggc
     8941 tctgataatc atatccgttc acaacgtcct tagcggaaag cccctcccct gacatcctca
     9001 tgtcaagagg tgcgtcattg tgataggaaa aacctctctc cgcattgtca agctgataag
     9061 aggaaacgcc cagatccata agaatgccgt cagcaccgct tatgccgagc ctttcaaata
     9121 tctcgtctat ctcagagtaa ttccctctca caacagtcgc aggatagcct gccaaccgtt
     9181 cagtcgcagt tttcaccgcc tcgggatctc tgtctatagc cacaagcctg ccgccctttt
     9241 cgccgtcaag ccttttagcg atctctctcg aatgtcctgc tcctccggca gtgccgtcgc
     9301 agtaaatgcc gtcaggtcga atgttcaaac cctccaaaca ctcatcaagc agtacgggaa
     9361 tgtggttaaa ttccattaaa aatcaagccc ctccagagct tccataagct cgttatcatc
     9421 aatgctgctg ttgaaatcgt cccagcgctt tttatcccat atctcacatc tgtcagatac
     9481 tccgaccaca acgatctcct tctcaagccc tgccacctct ctcagtgact gcggaacaag
     9541 tatcctgccc tgcttgtcag gctcgacctc gcacgcccac gccataaagc ttcgctgtaa
     9601 agctctcgcc ttagccatag gcagacttct gatcttttcg gctcttttct caaaatcctc
     9661 aagggaataa acgaacagac agccgtctat acctttagtt acaatgaacc gctcgccgat
     9721 aagctctctg agcttagtag gaaaagtcat tcttccctta gcgtccatag tctgattgta
     9781 agttccttta agcaagcacg ccagcaagcc gttcacctcc cttgaccgag ttaagcaatg
     9841 aaatgccact tttgaagcac tcatcaccac ttttttgcac ttttaaccac cgtacaataa
     9901 ttatacacta
//
LOCUS       DAWEMR010000091         9888 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2873_length_9888_cov_11.274077, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000091 DAWEMR010000000
VERSION     DAWEMR010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2873_length_9888_cov_11.274077"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2696 a         2200 c         2173 g         2819 t
ORIGIN      
        1 ataagggcgt aaagcttggc agaatacaga tattcaacaa ctggtctcct tatatggtgg
       61 aaaagcctga ggatacagtt tggataggcc ttgagtattt ctgcgccgag ggggacgatt
      121 tctggaatat gtccgaggag gattgcgtga aattcgccac cgccgagctt gttaaaatgg
      181 gagttatctc cgaaaatgaa gtcctcgatt cccatagaga aaaggtcaag aaagcctatc
      241 ctgcttattt tgatacatat aagcatttcg ataccatgat aaagttcctc gatactttcc
      301 cgaacctcta ttgcgttggc agaaacggtc agcatagata taataatatg gaccattcaa
      361 tgctcaccgc catggaaaca gccgaggcta taaagaccgg caaaaagagc aggaaggata
      421 tctggaacgt gaacacagaa aaagaatatc acgaagaaaa atctggaaac taatatgaac
      481 aaaaagaaag gtggacttct tctgtccctg cggaaagaca tgaacagaag agccataagc
      541 gacagacggc ttgaggcgat cttccgcacc ctcagccgtc acccttatat agttgcagcg
      601 ttcatatgcc ttgcactcac aggctttgga agctatagct ataagttttt aaacggcgtc
      661 attgtgtatg gcgttttcat gataatagag tttttctacc tcagacggtt tttgaaacag
      721 ctcgatgaca gcggaaaaca taccgcaggc tcagttttga cctttgcagg caagaccctt
      781 ttgacccttg cggcagtgaa ttatttcgta catacccagt ataaagcggc ttttatctgc
      841 tttgcaggac tttgcgtcgt gcttgttatg tatgtcgcgt tgctcaataa cgataaaaag
      901 ttccacgacc gtgtcacctg ccttgccttg gcaggagcag gctttgtcct gcgctattcc
      961 tatgtcttta tgtccgatat ctatacaaga cagaatgacg tgtggtattt cggcggcgag
     1021 ggcggtcacg caggctatat agagtatatc tataataacc acgccctgcc gaatttcgac
     1081 cctaccaccc tttggcagta ttatcacccg cctctgcatc atatcatctg tgcagtatgg
     1141 ctgaaattct gcgcctccgt aggtatagag tattacatgg catgtgaagc tatacaagcc
     1201 ttgaccctct tctatgtctg tgctgtaaca ataacagtct ataagatatt aaggctcttt
     1261 gaccttaaag gcaaagccct gactatcccg ttcggaatgt gctgtatgta tccatacctt
     1321 gtccttatgt cagggggaat aaataacgac cctctcatgc tgtgctttac agtgggcgca
     1381 atatattgcg tgctgaaatg gtatagagag cagactgcaa agaatatcgt caaagccgcc
     1441 ctctgtatcg gacttggcat gatgacaaaa atatccgtag ggcttatcgc ccctgccgta
     1501 gccatacttt tctttatggc gcttgtgcaa aatcgtaaga atatggcaaa ccgcctgggt
     1561 caaatgtgcc ttttcggtgt aatatgctgt cctcttggac tttggtggag cgtgagaaac
     1621 tacctgcgtt tcggcgtaaa accaaactat gtcccaagcc tttcaaatgc cgacgtccag
     1681 tatataggcg accttacagc caaacaccgc cttacagact tcagcttctc acagatcaaa
     1741 atagtctttg agcagtgggg cggcgaaagc tataaagagt ataaccctac catagcaatg
     1801 cttaaaaact ccatttttgg cgagggcata aatgaaactt tcttccccga aaacgcaagg
     1861 ctcgtgccat atgcactttt ctggatagcc ttggttcttg cggtcatagc gttcatcgca
     1921 atgctcatag tgctttttgt aaagaccgat aacgcaagat tcaccgaaaa actcatgctc
     1981 acagtggtct atgccactat cttaggcaac tattataact tctgcatcag atatcctttt
     2041 atctgcacaa tgaatttcag atacataatc ccatgtatgc tcataggact tataaatatc
     2101 ggacttttca cagacctttg caaccgctct gaaaaagccc cctgcaaagc catagtcagc
     2161 accctgagct atctttcttc tgcatttatc gtcctgtcct acataacata cttctttgtg
     2221 gcaagcacca acggataaaa ccccgacccc atgcaggggc gaacaacgtt cgcccgccta
     2281 gcttccccac aaactgaaaa atatcgcaaa aaaaataccg accgctcaac ccgagccgtc
     2341 ggtattttca ttatgccaat atagccttat ttcttagtgg taacattctt aacagccgac
     2401 caagcgccgt aatactttgt gccacctaca gttgtgtaag atctcacacg aacatagtat
     2461 ttcttgttgg cagaaagctt tgaaacagtg gtcttgtctg tcttgttgtt tgtgatagtc
     2521 actttcttag caccagtgaa ctttgagttt gtagcatact gaatttcata tccggtagca
     2581 gagcctttct gcgcccaatc cacaaagaat gcttttgact tagccgtaag cttctgtatc
     2641 tcctgcttgg caggatttat cttgaacgtc tttgtgataa ctccgccata cttgcccttg
     2701 cctgttatct ttacagtagc cttgccaact tttttgttgt ttgagtatga aactgtgtag
     2761 tcagtgccgt ttttaagaac agtgtttccc acctttacag ttacgttctg tgtaatagcc
     2821 tttcctgtga acgcctttgt ggaaatgccc gaaacagtag cctttgtgat gtcgttgtta
     2881 gctgtcttta tgctctttat atcagaccat gcgccgtagt ataccttgcc gtttacgttt
     2941 gtgtaagaac gaacacgaac atagtaaacc ttgtcccctg ccagaccgct tactgtcagc
     3001 gtgtcaggct tgtttgctgt caaatgcttt gaaactgcac cgctcatgtt tgcgtttaca
     3061 gaatactcaa cgtcatatcc cgttgcagag cctttctgcg cccagtcaat atagaagccc
     3121 ttgtattttg tttcaagctt ctgtatctgc tgttttgcag gaacaatgtc gaacttagcg
     3181 ctcagagagc ctgaatagtt gcccttgccg tatacataaa cgtttgacgt gccaacgttc
     3241 ttgttgtttg agtatgaaac tgtgtagtca gtgccgcttg taagcgtcct gcctgctacc
     3301 tttactgtaa ctgacggagt aatattgctt cccgtgaatg aaacgcttga agccactgaa
     3361 actgttccgc ctgccattgc gcgctttatg atagtgaagt tctttgatgt gtagcctgtg
     3421 tagtcgccca tgcctgtcgc aataacagtt gtaacgccaa cgttcagatt gtttctgtat
     3481 gagatagtgt aatccttgcc ttcctgaagc accttgccgc caaacgtaac tgtagccgca
     3541 ggctttttgt atgtgccgtc ataaacatag cttgtttctg aaagcgtaag aagtccgttg
     3601 ttaaggctct tctttgaaac aactatctta acgtcctttg aaacgctgtc ataaccattt
     3661 gcagaagccg taacagtaac atatcctgtg cctgtcttga gagcggtcac gtttccgttc
     3721 ttgtcaacag tgcatattga cggagtcgtg cttttgtatg tgagaacagc accgcttgtt
     3781 gatgagcttg cgccaatgtt cttgctctgt cccgcaacta tggtaacgct tgaagagcag
     3841 ttaagattta ctctgttgcc ataaggcttc ataagagcct cgataacgtc ctttgcaggg
     3901 cttgtaacag tgttgttttt tctgtttatg tagccgtgat attcactgtt gagaacaatg
     3961 ctgcttccgt tgtatactgc aaaagcattg ttgtcccaca gtacgcatgg aatgttgttc
     4021 ttctttgcaa gcgcagtgta gtctgttgcc cactttacac gctccgcagt gttgttcttg
     4081 tccgttgagc caaactcgcc gataacaaca ggtataccct tgtcacggaa attgcttttc
     4141 agcgtgctga aaaggtcctg aagctcgttc tttatagaag atgtatatgt agaggtcgag
     4201 cctgaaccaa cattcattgc aaagttgtaa gggctgtaag cgtgaactga tacagcgatg
     4261 cggtttttgt tgcctgaaat atctgtagga agcttgaagc ctgaaacagt tgccccgtct
     4321 atggaagcgt cgtaaccggg acacatgata agtctgcccc tgttgtttga gcctgtggct
     4381 cttatggtgt caacagcctt ctgattgagc ttgttaatgc actctatagc gtcctttacc
     4441 tctgacggaa tgctccactt gttgaaccac cactcatagc ctgtgccaat aagccgaggc
     4501 tcgttaaggg tttcaaatat aagatgatag tcataatcct tgaaatattc tgccatctgt
     4561 gaccatacgc tcgtaacaaa tttctcagag cttgaatatg cagaagacct tggataatat
     4621 cctgctcccg aagcgcttgc agaagacttt gtgtcatgat gaatgttgag aattacatac
     4681 atatcgtttt tgtagcagta gtcaactact tcctttactc tcgcaagcca tgcagagtcg
     4741 atagtgtagt tgtcacccga tacgtgcttg ccccaagaaa caggcactct caccgtgttg
     4801 aagcctaaag actttacctt gtctataaga gcctgagttg ttttaggatt gctccacgat
     4861 gtttccgtgt caagacctga accgcctgtt gcgtcaagag agttgcccaa attccagccc
     4921 agacccatat cctcaagtat ggcttccgtg tcagcgtttt tgagagtatc tcccgacaat
     4981 gcctgcacat ttatatcccc aaggtcaaga ccaataaata tggtcattgc cattaaaaga
     5041 gataaaataa agctcaggta tctttttgat actgatttca ttctttcccg tcctttcata
     5101 tataaaatat taacctcatc agtgtctttc attataccac atttctgttc aaattagaat
     5161 acgcttattt aacaaataac atacattctt tttgtgtata aacctcaaat gttcatattt
     5221 ttaatcacta tatgaaaaag tgccgattta ttaacgctaa tataattata caatgttaaa
     5281 attttagagt atattatctg caaaaaaatc agattttgct attgaaaata gagcagtatt
     5341 gtgctataat caaatgtgtt caaaacttta gtccaaagaa aggaaaggta tattttatga
     5401 aagttaaaca gtggcttgca tcatcacttg cagtgctgat gcttgtttcg accgtgggct
     5461 gcggagataa ttcctcaagc tcaaaggctg aaagcaaatc aaacaacagc tctgccgttg
     5521 tttcggaaac tgcaatgcct gaaaaggatt atgcaacaac gaccgacggc tcagcgaaag
     5581 tcaagatcga cggaacaaaa ttcatggtgg gcgacaagga gctttggttc aatggagtga
     5641 acgcaccatg ggataagtgg aacgactttg gcggaggctt taattttgag ttctggcagg
     5701 atcatttcca gaagcttcac aattcaggcg taaatgccgc aaggatctgg ataatctgca
     5761 acggcgacgt tggtatggct atctcagcag acggtacatt tgacggtgca acaacagctc
     5821 attgggagga tcttgacaat ctgttctatc ttgcagagca gtatcagatc tatataatgg
     5881 caacagttca gtcctttgat aacttcaagg atcagaacca gaattatcag gcttggagaa
     5941 cgctcattca ggacagcgac aagacagata tgtttgtaga taactatata gttcctctcg
     6001 ttcaaagata tggcaagagc gactatttct ggtctgtgga cctttgtaac gagcctgact
     6061 ggatagttga aaatgaagag tgtggcaagc ttgattggct ctatctggag cagtattatg
     6121 caaaggcagc cgcagctatc catgcaaatt cagacgttct cgtaaccgtg ggcatgggta
     6181 tgataaagta taactccgac agccagcagg gcaacaaaat atccgacagt gcgcttcagg
     6241 acgttttgag cggtgataag tatgacaaga gccttgccta tgttgacttc tattcaactc
     6301 attggtacac ctggatgcag ggaatgtggg gatatccttt cagcgaatct ccaacagact
     6361 ttggccttga cggtacaaag ccttgcgtta taggcgaatg cccggccgtt gcaagcgaca
     6421 gcgatttcga catcacctca gcttatgagg acgcttacaa caatggctgg aacggcgtgt
     6481 ttgcatggaa aacaagcggt caggacgacg gctgtggact gtggctggat atacagcctg
     6541 ctatagagaa aatggcaggt atctgcgagg ataagatatt ccctaacggc aaaaaagctg
     6601 tataaacaaa ctttgataaa ataatcacaa aaagagccga cttttgcggc tcttttttgt
     6661 tgctcttttt tgtgaacaaa ttgtgctgtc tgttgactat agtgcggcat aatgctataa
     6721 taagatacgg aaaatcaatg acgatttgca gagagtaaaa tgctgtagat cgttattttt
     6781 acgcattaac gtcatataat gacggcgtgg aaaggaagta aaatcatgga agaatacagc
     6841 tacatattgt ttattgcact tatactgctt tcgaccaaaa ttctcggact tgtaacaaag
     6901 aaaatgaaac tgccgcaggt agtcggtgcg ttgttggcgg gcgtaatact cggacctgcc
     6961 gttcttaatg ttgtaaaaga aactgagttt atctcagaac tgtcagaaat tggcgttatc
     7021 gtgctgatgt tcaccgcagg acttacaaca gacgttaagg agcttaaaag gactggtaaa
     7081 gcgtcattca taatcgctct tcttggcgtt atcgtaccac tgttcggcgg acttggactt
     7141 gccgctgcaa tgggcgtagg caagggcgat aagctcgcct gctcaccatt cctgcagcac
     7201 ctcttcatag gcgttgtgct tactgcaaca tcagtttcta tcacagtaga aactctgaaa
     7261 gaacttggca agctctctac aagagcaggt aatgccattc tcggtgcggc tatcatagac
     7321 gatatcctcg gtatcatcgc attgaccctt atcacaagcc ttgcagatac aagcgttcac
     7381 gttgcaacag ttcttattaa gatagtagcg ttctttgcag tggctatcgg ctgtacagtc
     7441 gtttaccatg ttgtgttcac aaagtggaga aaaagagata aaaaagacct tagacgtcac
     7501 gttatcattt ccttcgtttt ctgcctgctc atggcgtatg tagcagagct tttcggcgta
     7561 gcagatatca caggcgcatt tgttgcaggt cttgcaattt caagcgaaaa ggacgtaaag
     7621 tatatctcaa acagatttga aacactttcg tatatgttcc tttcaccagt gttctttgca
     7681 agcatcggtc ttacaatgaa cggtatcaac atgaacggca aaatggtgct gtttacagtt
     7741 ttgctccttg ctgtggcaat aatcacaaag ataataggct gcggactagg cgcaaaggtt
     7801 tgtaagtata caaatcagga aagcttgcag atagcaatgg gtatgatctc acgaggcgag
     7861 gtcgcactta tagtagcggc taaaggacag aagcttggac ttatgggcga cgaggttttc
     7921 gcacctatca tcatcatggt ggttgcaaca acgataatca caccggtatt cctgaagttt
     7981 gtgtttaagg ataatcatga agaacacgtt gaggatgagc ttatgaagtc tgtggagggc
     8041 ttctcacagt atgaacagga gctgcaggaa ctctcaagaa aagaaaagta atgaaaaaag
     8101 caaagagcgg agcaaaaaac tccgctcttt ttttgtacaa aaaatccgct gccacgaatg
     8161 atagcggata tgggcggcga taaaaaaatc cgctgccacg aatgatagcg gatatgggtg
     8221 caggggcacg ccttcatatt ttatcacagc gtaaaaaatg atatgggcgg cgataaaaaa
     8281 atccgctgcc acgaatgata gcggatatgg gtgcaggggc acgccttcat attttatcac
     8341 agcgtaaaaa atgatatggg cggcgataaa aaaatccgct accacgaatg atagcggata
     8401 tgggtgcagg ggcacgcccc tgcctaaaga ttgaacatga tctgattgag tacgctctcc
     8461 agatattcct gtctgccgct tgaaagtgag tcagtaactt cgcccttctc aactgcatac
     8521 ttctcaaggc tctccatgtc agccttacct gcaatgatgt ctgcaccaat gcctgttgtc
     8581 cagcttgcat atctgtcatc aacgaacttg tcaagtctgc cgtcctcaat gatctttgca
     8641 gcagccttaa gaccaagtgc aaatgtgtcc atacctgcaa tatagctcag gaaaatgtct
     8701 tccattgtgt atgagcctct tcttgcctta gcgtcaaagt tcagaccgcc gtttgtgaag
     8761 ccgcctgcct tgagaacttc gtacatacaa agtgtagcgt cataagcgtt tgtagggaac
     8821 tggtctgtat cccaaccgag cagtgggtcg ccctggttag cgtcgataga accgaataca
     8881 ccgttatctc ttgcaactct cagctcatgc tggaatgtgt gctgggcaag tgtagcgtgg
     8941 ttagcctcga tgttcatttt gaagtccttg tcaagaccat attttctcag gaaaccgaga
     9001 actgtagctg tatcgaaatc atactgatgc tttgtaggct cctttggctt tggctcgatg
     9061 tagaaatcac cagtgtagcc ctttgagcga gcgtaatcaa cagtcagctt cataagtctt
     9121 gccatgttgt caagctccag acccatgttt gtgtttagaa gagtctcata tccttctctt
     9181 ccgccccaga aaacgtagcc cttaccgcca agcttgatag tagcgtcaac agcgttcttg
     9241 atctgagcag ccgcataagc gaaaacatca gcagaaggtg atgtgcctgc accatgcata
     9301 tatcttgggt gatcgaagca cttagcagta ccccaaagaa gcttcttagt agggtcagca
     9361 gccatttttt cagctatgta gtcaactacc tcagcaagct ttgcgttagt ggtgccgaga
     9421 tcaccgtact caggagaaag gtcacggtca tggaagcaga aatagtcgat agaaagcttg
     9481 tccattatct cgaaagcaac atcgaccttc ttcttagctc ttgcctcagg agcagcctcg
     9541 ccccaagtct tgtcagtagt gccgcagccg aacatatcag tgccgtcgcc gcccattgtg
     9601 tgccaccatg aaagagcgaa cttgagctgt tctctcatag tcttgcccat tacaacctca
     9661 tctgggttat aatacttaaa agagagggga ttagttgagt ttacgccctc atacttgatc
     9721 tttggaatat ccttaaaata ctctgccata attttgacct ccgaatatat tattgtttga
     9781 tcttaacgat taaacgatta caatacaata atacaccaca tcgaacccgt ttgtcaactg
     9841 tttttatcaa aattgcatat attgatatat attaaaattg tgggataa
//
LOCUS       DAWEMR010000092         9841 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2881_length_9841_cov_9.582056, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000092 DAWEMR010000000
VERSION     DAWEMR010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2881_length_9841_cov_9.582056"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    613..622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5968..5977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7673..7682
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2646 a         2228 c         2203 g         2734 t
ORIGIN      
        1 ctgtctccaa tagttccttt actgcctgtt ttccaaggga ctccgtttcc atgccgtttt
       61 cggcaagcca ctccttcatc tgctgtacgg aatttgggtt gtcaagcatg gtaagtgcct
      121 gaatttcggc agtgatctcg tttttgatgt cctcattgat cgtgatagcg tgacgcacaa
      181 gctccatatc tacaccgata ccacgatcat tgatctgctg gtcgaggtga tattcattcc
      241 atacaaaatc cggtacggga tatttggcga gcctttgctg aatggcgatc tccacctcaa
      301 catcccggca gttgtaggac ttgaacagtt cccatttgcc cggatgtacc ttgggatgat
      361 gacggttgcc gtccttgtcg ggaatacaga agaacttgat gaggtctttg ccctcggaca
      421 tcttctgctt gtccagaccg agaactgcac caacaccggc aagggacatg ggcagaccga
      481 gcgttgccgc ccagaccatc gtgcagtgcc actgtgtcgg gtcaagatat gttcccttgg
      541 gaagtccgag atgcttggaa atacagatgc gctcgaacat ggcattgtag gcggtcttga
      601 tgacattcgg gtnnnnnnnn nnaatatcgt cggggagctg ttcaccgttt gcaaggtcaa
      661 tgacctgcgg cggatgtccg tccacgctgt agccgaagaa cagaatctca aaattatcgg
      721 attccgcata gggataaact cccgatttac tcagatcaac atccgaatag gtctctatat
      781 caattgaaag tattctcata gttcaaataa tcccacccaa gccgaagccc agcgccccac
      841 ccgtctgtat tcttagttca ggaaatcgtc atcgtcctca tcatcgaggt cagcaaagtc
      901 atcttccgca ctggaacggc taccgagagg ctcaccgtca cggagcttct ggagattacc
      961 gagaccgcag gcgataccac gggcagttcc ggtattgtat gcgtagaaat tgatagatgc
     1021 tctgccgtaa acgccggagt acacctcgct gtgagtaaga atcggctgac gatcagcatc
     1081 cacgatctca ggtgcagtga tgctgttggc attgacgaag tatgcgtttg cgtaggcttc
     1141 atcatctgga cgctcgatgt caccgtcacg aagaggtgtc ttgatcgctg ccagagccgg
     1201 tacggacttg ccgttgccct tgagcttgcc tgcaccttcc tcgtaggcag cctgaattgc
     1261 tgccttgatc ttctgaatgg tgacggtgtc gctcttgggg atgatcaggg aaacggaata
     1321 cttctccttg ccaccgtcct gacctgcttt cggttcccat acatttgcgt aagaccagcg
     1381 ggtattcaca ccggtcacga ccttggtcgg atttgtcatc ttctttgcca tgttactgtt
     1441 cctccttgaa atcttcattt gcatcggaat acacgggacg cttgtccgtt tcgggaacga
     1501 gcgtcggtgc gccctttggt ttttctacat atccgctgag aatttcagcg aatttcttct
     1561 tgccgagcag cttttccatt gcagtgatgc ccatgatctc aggcgcaccg taggggtcat
     1621 agcctgcatc ggtgacagct tttgcggctg catccttgtc gatgtatttg cggacgctgc
     1681 gaccttcgac cagcttatag ccgtcataat gaactccgct gagagcctgt gcaagagcat
     1741 actccttgac atcatttgcc catgtgacca gctcactcgc cttggagaga atgattggaa
     1801 tttcatgctc ctcaaggttg tcgggaggag cgaactcata ctgtgcaagg agcaaattct
     1861 gttcagctct tttgcggcag acagcctttg ccttacagaa acggcagtga cttccggcac
     1921 agaattctcc ctcaccgtta taggcaagct gtgcggtcgg tttcagcgtg tgttccgccc
     1981 attcgagcag ctctgccacg ctgatctccc actcatctac attttcaagt ctcggctgat
     2041 agatgataag gcgaacggtg ttgatgtcat acaggaaatc aaacatcgca agtgtgccca
     2101 atgcgtaggt cttgagctgc ggattgtcgg ttgcttcgac ctttataccc atcccgtgct
     2161 tgtaatccga tatggtcatc gtgccgtcag caacaatgat gcagtcggcg gtaccgaagc
     2221 attccgggat atattcggac acatcaactc gctgttcgag catgatctcg gtcgtggtgc
     2281 aattctgctt tgcggtctca tacagctcca tgatgtaagc cgcatagccc tcggcacact
     2341 cctgcatttc agcgttgtag aacttcagct ttttcttcgg gttacggact ttctcgccca
     2401 gtgccttgcg taccagatat tcgcaaagct cgtgagcctc tgttccctcc gcagcaaagt
     2461 cgctttcggt atcggggaac tgctccgaga atcttgcgga cggcggacag gcaagcactc
     2521 tggcgatgga cgaaggtccc acccacgcgt gtgctttcgg cggcattaca tcatcgcctc
     2581 caattctgcg agcattgcgg gatagacctc cgctttcagg tcggagaatt tctgaccgcc
     2641 gtacttctgg atgagttccc ttgctttcgc cttatgtccg gctctgctga actcggacat
     2701 cttggcgaag agcacctcgc tgacatcggg ctgcggaggt tctgcttcct taatttcagg
     2761 ctgatctgtt tcgggatcgt agatcggaac gaagtcgtcg gggttgatag gctcggcggg
     2821 tttctcacag gcgatatgct cattggttgt cacagcgttg gcatattccg caaggctgtc
     2881 tgcaagatgt ctcatgtcgc tgacaacatc cagcaaaagt ttgatttggc tcatgttttt
     2941 cactccttta cttttttacg atttcaaaca gcaccccaat agcggtgtgc atcttcttga
     3001 tgagtgccgc attgactctg agctgtgcct tcgccacctt catcgccatg atggtgaact
     3061 tcgggatgtt cttatcggac agggcgatgt caagtatcag cacacagcag gcagtgtcat
     3121 ttaaggtctt ttcaacctct gcggaactca attctatatg atccattgtc ctcactcctt
     3181 cctaagaaca acaaaaaagc ccgatttgac aataggaatc cgagggtaga cagaatacag
     3241 ctttacagag gttttcttag acttagcccg taaaaacggg atgccctaat aagctgatat
     3301 tccgagtacc tcaagcccta ttgcaaatca ggctttttga tgactctttc gttttcagtc
     3361 ttgatgttgt ccttgactgt gtctatatca taccatagtt tttcgtaaaa gaacagttaa
     3421 taaaaacacg agaaaattct tgttgaatat gctttgtaat tggttgcaat tagtgtttga
     3481 attgtttgtt attactaaaa atatgcgtat tttactgcat attcggcaga aatatacgca
     3541 ttttttcgag aaaaattcgg gttttaaatc cgagttcatg caaaaaaaat tcaggcagtc
     3601 gtttcaactg cctgaatgat tgatctatac tgctttttgc gccttgctgc gtggtttata
     3661 tcccgtagtc catgtcgact tgccaatact tctcaaataa tcattgacct gataaacatt
     3721 aaagccgggg acagtattct taaaccacag atatgtctta tactcctcgc tgtcaatttc
     3781 tgagatcagc ttggtcatga tgaggagttc gtttcggtag acttcttcca gattcaaagc
     3841 cgtgaagaat gccagcatcg ttccgaggcg aggtgtgtat tttttcttgc tgcggagagc
     3901 agtcagtgtt tctacgctgc agtcagcggc ttccgcaacc gcatcctccg tcatgccgga
     3961 acgcttcatc aggaatttga cggtttcgcc tctggtcatt tttcgtgtct gcttgacgcc
     4021 attttcttca tactcaacag tcagaaggct gagaagatat tcgtcacggt tggcttccag
     4081 tgctttcaac tgctcggcac tgaatgccat ttcatagact cgagcctgct gttccttatt
     4141 tagttcgcca aaggtatatc gatagttttc cttaccatac acacggcgga atggcagaca
     4201 gcattccgcc atattctttt ttgctttttc tgtcagccac cagcgaccgt tgaaattgat
     4261 aacatagtcc ggatgattca ggcaaaggtg tccgtctgca tagacaaagc tgcctgaaaa
     4321 gactaacttg tcaaatgtga ggtttgtgcc gagtattttg gacatttcct caattggaag
     4381 ggtgtatgtc caatcatctg ggaaatcatc cgggatctca tgtgccttgg catacgacct
     4441 gtcgccccac tcaaatacac ctcgaactcg cttgaatccc aaatgatgca agcgattctt
     4501 ggcggtcgtg cgataggagc cgtatgtttc agtgatatgc tcgatcagtc tttcatagcc
     4561 gtagaggtcg gtatgatgaa tatctctgcc aagctcatcc ttaaaattct caagggtaat
     4621 atcaaggttt tccttgtaaa ctactgcacg ggctgagatc gcatttgcct gtatctccat
     4681 gctgcgtatc gtgtcctgct gatcttccga cataaagaac tcatcaaaat ccggaatttc
     4741 cagctttaca agcgaccgat aataatattg aagctcataa aacagcatgt gatactcaat
     4801 atgaatgcac tcgtgaagta tattcatcag ataatcaggg tcacacttac tgctgacaag
     4861 aattgtgccg ccctctatca gcttctgaac agccttgtca ccttcatata cagtgaccat
     4921 tttggtatgg aatatcgtct tagcttttac ttttccattc cttgaaagct gttccacctg
     4981 aatcctgtag cccatagcct ttacaagact ttctatatca attttacacg gaccttccaa
     5041 tgcttccggc tgatatttca ataggaagtt gtaggcgtaa gcgtcataat gagctttgcg
     5101 aatatatgga acaagacagt catcaagatg atgcttcaag cgcagtcgtt caccacgata
     5161 aatatccacg ccgagaaaat aatccgaggg agagtaaaac gcatggtagc cgttgacgca
     5221 gtatttctga tagtagtcct caccggtaac aggatcatag aagtggacat cgcaaatgac
     5281 atacattgca aaagagaagc tgtcgagctg gtcgtagtaa cctttatcga tcagcacatc
     5341 cacgatgaca ggcttgctgt aagcaccgaa atacggtacc ggcagtctgc cgtgggcaaa
     5401 ctcccgtcga ataaattgtc cgtattctac aacatagttc tccttgagaa tctgcgtaaa
     5461 ggaaactatc tccggtgctt tatagcatcg gggatttcgc actttatatg aaaagcccgg
     5521 acgcagcgga tccggctgca tcagaatggg cccgatttcg tagttcattc aaacacctcc
     5581 gttcaaaaaa ggtgtctcct cataagttct tccacacggt cacagcaaca ccttgtatca
     5641 gacattccgt gacatatatt ggttcataat catcattttc aggctgaagc cgtattctct
     5701 ttttcttcgg ctccggatag tatctcttga gcgtggcttc gtcaccgatc agagcgacaa
     5761 tgatgtcacc gggttcagca taattctgct gacgcacaac aacagtatcg ccgtcatcaa
     5821 tatctgcatc cttcatagaa tcgccgtctg ctgtcaggag aaagaactct ccgctgccga
     5881 gtaacgatgc aggcagcttt acatattctt tgacagcact atccgcaaag attggcggtc
     5941 cgcaggcgat gtttccgtaa atcggacnnn nnnnnnntgc tccgagttgc atttttcttg
     6001 tagcgtatcc cttttttcca ttgtgttcga gtctgccctc atctgtcatt ttgagaaggt
     6061 aacgccccac cgatgacttt gatattcccg taccttctgc tatttcagcg atttccggtg
     6121 ataatcctgt ttcttcatag tattcgtcaa tgaagttttc gattgcatcc aaatttgcct
     6181 gaatagcttc cgcagatatt cgcattataa tcacttccac ttattaggga ttacattccc
     6241 ttttcttata ttttatcaca ctatacagca attgtcaata ggaaaataga acacttgttt
     6301 tgatgtgcgg aatcatgcac atgacatagt aagaggggat aggaaaaagg atgcacctga
     6361 aaaagtaaaa aggcatataa ttgcggtatt atataactga aagctattga aaaaatctga
     6421 gtagtgttgt ataatatagg tatgaaaaca ccatcttgag taaacggagg agaaaatgag
     6481 tattttagat gagatcatag gcagcaccta tgagtatatt gaaaaaatgc cgaaggaaca
     6541 gcgaaagcag tatgggcaat ttttcacaag caaggaaact gctcgtttca tggcagaatt
     6601 atttaatatt cccgaaaata caaatgaact gtctatcctc gatcctggag caggctcagg
     6661 tgtgctttct gccgctttga tagaacgtct gcaaaactgt cccaatatca aatccgtata
     6721 tttgacttgc tacgaaacag acgataatat cgtagaactg cttgaaagca atctggaata
     6781 cataaaaaac aacacttctt tgaaaatgac gtttgatatt gtcaggaaaa atttcattac
     6841 atcacaagaa gataattata atgggacatt tttagctgat cctgctccaa ctaaatatga
     6901 tatggtgatc ggaaatcctc cgtataagaa gatacctaaa gatgctgccg aggcattgtc
     6961 aatgccggat atttgctacg gagcgcctaa cctttacttt ctattttcac agatgtcact
     7021 ctttgatctg aaagagaacg gggaaatggt ttatattatc cctcgttcat ggacttccgg
     7081 agcttatttc aaggcttttc gtcagaaatt cctgtcacaa ggtgcgattg agcatatcca
     7141 cctttttgta agccgtgaca aagtttttga aaaagaaagt gtgctgcagg agaccataat
     7201 cataaaggtc aaaaaaactg aagtaaagcc tgaatatatt actgttacct ctaccaacag
     7261 caatcaggac tttacagaaa tcacaacatt ttctgcaccg tataagactg ttatttcagg
     7321 cgacgattgt tatgtttatc ttgtaacgaa tgaggatgaa gtgaacacac ttgactgtct
     7381 caataagttt gatgatactc ttccaagcct cggtctgaaa atgaaaacag gactaacagt
     7441 tgacttccgg aatcatgaag ctctccgcag tgaagcagag aaacaggctg tacctctttt
     7501 ctattcacag catattcagg acggaaaggt ggtatttcct atcggtaagg aaaatgaata
     7561 tatcgtcact cagcagcaag gcttactgca gaaaaacgca aattatctct ttgtgaagcg
     7621 atttacagcc aaagaggaac atcggcggct gcaatgcggt gtatatattg ccnnnnnnnn
     7681 nnattgcccg taaacatacc ggttatgaca ggatcagcac acagaacaag atcaatttta
     7741 ttgatggatt gaaaggactg tccgagtgcg ttgtttacgg tttatatgtg ctgttcaact
     7801 ctacccttta tgattcgtat tatcgcatct taaacggctc tacgcaggta aactcaacag
     7861 aggtgaactc catgccgatg ccgcctatga catctatcga agcgatgggt aaagagctga
     7921 taaaagtaca tgatatgtca gaagcatctt gtgaccgtat tttaaggagt tatgtatgag
     7981 caaaattgaa gaagcaagaa aaatactaag tgaacttaac gtccctaaga aacagcaggc
     8041 tgacctgtgc tgctatgttc ttctggcaat gagtgatata aaagaaggaa tagcatggac
     8101 agaggctacc aataatctga taaggataca tgatgtgctt gtattcacaa gagaaaatta
     8161 tggggtcgaa tatgctgaaa acagccgtga aacattcaga aaacaggcaa tgcatcattt
     8221 cagaaatgca gctttcattg aagataacgg caaagctaca aacagtccta attaccgata
     8281 ccgactgacc gatgaatttc tcgcacttat tcaaagctat ggcactgact tttgggagca
     8341 gaaaaaacag gatttccttt taaaccatga atcgctcgta aatatgtatg cttcaaagcg
     8401 ttctatgcag aaaatgcctg ttattatcaa tggtgcaggc tttactttct cgcccggaaa
     8461 acataatgaa ctccaaaagg cgattataga ggaatttgca ccgagattcg ctccaggctc
     8521 ggaatgtttg tatgttggcg acacaacgga aaaagacctc gttaagaatg tagagaagct
     8581 gtcagggctt ggctttgaga tcacactaca tgataaaatg ccggatgttg tcctttactc
     8641 cgctgaaaag aactggatat actttgttga atcggtaaca tccgttggac cgatggatcc
     8701 gaaacgcatc agagaaatcg aggaaatgac tcaaaatgtt actgccggca agatatatgt
     8761 aacagctttt cttgatttcg ccacatacaa gaaatttgca gaatctcttg catgggaaac
     8821 agaagtgtgg cttgccgaaa tgccggatca catgattcac ttgaatggcg ataaatttat
     8881 gggcccaaga aataagtaaa tgcgaggcga tgcaacatgg taagcagaac ttttgattta
     8941 ggcagcttgg aaacgtacaa gtacgtcgtt gttctctcag agtacaacgg aaaaatatta
     9001 ctgagccgcc ataaagaccg cactacatgg gaaacgcaag gtggtcacat cgaggatggt
     9061 gaaacgcccc ttgatgctgc aaagcgtgag ctgtatgaaa aatccggtgc gattgacttc
     9121 gatatagagc ctctctgcga ctacagggca tggaatgagg acactgggca tggtgcgaat
     9181 ggtgtagtgt tcaaggcaat aatcagggaa ttaggcaata ttcctgagag cgaaatggca
     9241 gaggttcaga cttttgattc tctgccgaat gagcttactt atccggcgat tacacccgtc
     9301 ctgttccgtt accttgaaac gggacaaaac tctgtggtgc ttcatcggat gaagctcaaa
     9361 gccgagccgt tccgcaagat tggggacgga cttaaaatga tagagcttcg cctaaatgat
     9421 gaaaagcggc agaacgttca aattggcgac tatattgagt ttactcttat ggatgataca
     9481 tcacagaagc tgactacacg agttgttgct ctgcatcatt tttcgtcttt caaggaaatg
     9541 tatgcttcgc tgccgaaaga aaaactcggc tattctgccg atgaaacgtc tgctccagac
     9601 cacatggacg cttattattc gaaagaggaa caggaaaagt acggtgcatt ggggattgaa
     9661 attgagctta ataactataa atgagcatcc ctaaaaaatg cacaaaaaat aaggacaaca
     9721 gtcccaaaaa gagttataat gtacttgtaa cctacattaa ctcaaaggag tgttgtccta
     9781 tgaaaacaag tatacactat tctgaagaaa tttgcaagag attggaagca aaaaaattga
     9841 c
//
LOCUS       DAWEMR010000093         9783 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2903_length_9783_cov_13.250000, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000093 DAWEMR010000000
VERSION     DAWEMR010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2903_length_9783_cov_13.250000"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2986 a         2060 c         2238 g         2499 t
ORIGIN      
        1 ataaaaatta taaatatatt ttctcccatc aaaggaaaga aaatcgtaat ttttcacaaa
       61 aagcctataa aacacaaaag caccttgcga aattttccgc aaggtaactt tcctaattga
      121 gaattatagc acatttttgc cgatttgtca agaaaaaagt atcattcaca ggttacgcct
      181 tgtacggtgt gccttagatt aaatgattta tgtttttggc tgttttacac aatattacgc
      241 cactgatatt ttacataaaa atagttgaaa tctcggtata aaagttgtat aatatcaata
      301 atcaaagata taactttgat aacagagatc atatatttga aaggcggaat aatcaaatgg
      361 ctaacagaat gatacttaat gaaacttcat atttcggtgc aggctcgatc agcgagatag
      421 ttacagaggc aaagaagaga gcttttaaaa aggctcttgt tgtaacagac cctgacctta
      481 tcaaatttaa cgttgcaaag aacgttacag atcttttgga ggaagcagga cttgcttatg
      541 atatattctc tcaggtaaag gctaacccta ctgtaaaggt agtaaaggct ggtatcgaag
      601 ctttcaaggc agcagatgct gactatctta tcgctatcgg cggcggctca tcaatggaca
      661 ctgcaaaggc tatcggtatc atcataaaca accctgagta tgctgacgtt acttctcttg
      721 agggcgctat tgacacaaag aatcctagtg ttcctataat cgcagttcct acaacagcag
      781 gcacagccgc agaggtaact atcaactatg ttatcacaga cgaggagaaa aagagaaagt
      841 tcgtttgcgt tgatccacac gatatcccag ttgttgcagt tatcgacgca aagatgatgt
      901 catcaatgcc aaagggactt acagcttcaa caggtatgga cgctcttaca cacgctatcg
      961 agggctacac aacacttggc gcttgggagc ttacagacat gatgcacctc aaagcaatag
     1021 agataatctc acgttcactg agaaaggctg ttgagaacga tcctcaggga cgtgaggaca
     1081 tggctctcgg tcagtatatc gcaggcatgg gattctcaaa cgtcggtctt ggcgttgttc
     1141 acggcatggc tcacccactt ggcgcattct atgatacacc tcatggtatc gcaaatgcag
     1201 ttcttcttcc gtatgtaatg gagtacaacg ctgaatacac aggcgaaaag ttcaaataca
     1261 ttgcagaggc aatgggcgtt gacacaacag gcatgagcca ggctgaatac agagcagccg
     1321 cagtaaacgc agttaagcag ctttcaaagg atgttggaat ccctgagaag ctccacgaga
     1381 taggcgttaa ggaagaggat cttcaggcac tttcagagtc tgcattcgca gatgtatgca
     1441 caggaggaaa tccaagacct tgcacagttg aatctatcct cggcatctac aagacagcat
     1501 tctaattcaa aaatctttta tctgccctcg gttttcgggg gcagattttt attgcccgaa
     1561 aaaaatattt atggtataat acatttaaag agagtttaaa gttcataggg tatcatatat
     1621 cgtaagatat tataatgact tatagggagg aattttaatg agattacttc ttgccgagga
     1681 cgaagtggag cttgcaaatg cgctttcggc agtgcttaaa cacaaaaact attctgttga
     1741 cgctgtttac aacggcatcg acgcatacaa ctatgcaatt gcacaggatt atgacggcat
     1801 aatacttgac attatgatgc ctggtatgga cggcatacag gtgcttacaa aactccgtga
     1861 aaagggtatc ggctcccctg ttcttctgct tactgcaaag tctgatattg acgataggat
     1921 aaccggactt gattcgggtg ctgacgatta tcttacaaag ccatttgcaa tgggcgagct
     1981 tcttgcgaga ataagggcaa tgacacgccg ccgcactgag tttgcaccct ctacccttac
     2041 tttcggaaac gtttctctca acagacagag ttttgagctt tcaggtccta acggctctca
     2101 gaagcttggc aacaaagagt ttcaaatgat agaagccctt atgctaaatc acggcacgct
     2161 tatttccaca gagcgtttca tggaaaagat atggggctat gactctgacg ctgagataaa
     2221 cgtggtgtgg gtgtatattt catatctgcg taaaaagctt ggtcttatcg gtgcagatgt
     2281 cgagataaaa gccacaaggg gcgtgggcta cacattagag gaaaagcata atgattaagc
     2341 gtttacagcg gaaattcgtt gttatcgccg ctgtgtgcct tcttatcgtg gagtttgcca
     2401 taataggcgg cataaatgga gtgaacatct atcagaccaa caagaatgca gacaatctgc
     2461 tgaacataat aatcgaaaat gacggcagat ttcctgagtt cgaccccaag ggcatgaagc
     2521 atgacaagcc gcaggataat tcagacagct catctccccc tgagagcaaa aagcctgata
     2581 agcacagagg ctttaacgag gaaactaagt atcagacaag gtattttgtt gtatacgccg
     2641 acagcaatgg cgaaataacc aaggtagata caggacacgt tgcggcggtg tcaagttcgc
     2701 aggctataga atacggcaaa agcattcttg acagcggcaa gacttcaggc acgtcaggaa
     2761 actaccgcta caaaacaggc gacactgaca aaggcaagat aatagttttc atggactgcc
     2821 gcagtgacgc cgcaactaaa aaccgtttcc ttcttgtgtc atgtgccata ggggcggcag
     2881 gatatatact tgtatgtctg cttatcataa tcttctcaaa gcgtgcaata agacctgttg
     2941 tggaaagctt tgaaaagcaa aagcagttca tcaccgacgc agggcacgag ataaaaacgc
     3001 cgctggccat aatctccgca aacacggaag ttcttgaaat ggtgtcagag ccaaaccagt
     3061 ggacggtgag cataaaaaat cagataacaa ggctcaacgg tctactgaaa gagcttttaa
     3121 gccttgcaaa aatggaggag gaaaagcctg ctcttaccat ggctgatttc aatatttctg
     3181 acgctgttta tgacgccgct tcgcctttta ctacccttgc ggaaacaaag ggcaaaaagc
     3241 tcaccattga tgttgccaac ggactttcct atcacggtga cgagggtgcg ataagacagc
     3301 ttgtttctat cctcacggaa aatgcagtaa aatatgctga cgaggatggc gagataatag
     3361 taaagctttt cacttctcat aatggcaagg aaactgacct agaagtttca aatacctgca
     3421 aagaaccacc gcaaggcgac ctaacaaaac tttttgacag attttatcgt gacgacagct
     3481 cacgttctcg gtcaaaaggt gagaaaaaag gcggctacgg cattgggctt tctattgcac
     3541 aggcaatagt caggtcgcac aaaagcaaga tatcatgcaa agccgaaaac ggcatgatga
     3601 tatttacagc acagcttaaa taactgcaaa gcagagttgg tcaccagccg ctctgctttt
     3661 ttataatatg acatttgtca catgaagata tgactttgct cactagcttt ttggcggata
     3721 gtattgtata atgataatac aaactaaggg aggtttttct tatggctgaa actgtagtaa
     3781 aaatagacaa tctggtaaaa agatacaagg agcttattgc tcttgaccac tttgatcttg
     3841 aaataaaaaa aggcgaggtc ttcggacttc tcggtcctaa cggttcgggt aagaccacca
     3901 cgataaactg cctgctctca ctgttggcat acgataaggg cgacatcgag atattcggtg
     3961 aaaaaatgtc ccctgtgaac tatggggtaa aaaagcgtat tggcgttata atgcagaatg
     4021 ttgcggtgtt tgacgagctg actgtgtatg agaacattga ctatttttgc gggctttaca
     4081 tcaccgacaa agccacacga aagaaatatg ttgacgatgc catagctttt gtgggacttg
     4141 aggactttgt gaagttccac cctaaaaagc tttcaggcgg tctgctcaga cgacttaaca
     4201 ttgcctgtgg tattgcacac aagcctgagc ttatcatact tgacgagccg acagttgccg
     4261 ttgacccaca atcaagaaac aagatactgg agggcatttt acagctcaac cgtgaaggtg
     4321 cgactataat ttacacctcc cactacatgg aagaagtcga gcagatatgc acccgtatcg
     4381 ccataatgga taagggcagt aagatagccg agggtactac agaggaactc aaacgcatga
     4441 taaagaacac cgaaactatc accgccgaga tactccagct tcctgaaaac gtccgtgaac
     4501 aaatagaagc tttggaacac gtttataatg tttcctatgc cgacaacaag ctcgttgcac
     4561 gctgtacagg gggacggcac aatcttattg agatactgaa aatattgcag gaaaatcagg
     4621 tgggcttcgg cagcgtttat tctgagcagc ctaccctcaa tgacgttttc cttgaaataa
     4681 ccggcaaagc tctcagggat aaggagtgat agaatgttct caaacatatt caaatacgaa
     4741 tttaaaagct gtctgcgtca aaaagccata ctcttctggc tcatagcctt tcccataata
     4801 ctgggttgct tttttaagat ggcgttttca ggaatatacg aaaaagatgt tgtattcaat
     4861 gcgatacctg ttgcggtgac aaatatagaa gaaaacgata tccttaagca ggtgcttgaa
     4921 cagatatcaa atagtgatga ggctcttttc aaggtgacgt atacggatat tgacaaggca
     4981 gaagaaatgc ttaaaagcga agatataaaa ggcattataa aggcttccga cctctccctt
     5041 gagtttggct caacaggtat ccagcagacc attgtaaaga ctttcataca gcaatacaaa
     5101 actcaggaaa agataataac cgacactgca aagaacgctc cgcaaaagct tgataaggtc
     5161 atagcttccc tttctgagaa gataagccca aatgaggata tacctctcca cggcaatcct
     5221 gacataacga tacagttttt ctataatctc cttgccatga cggcactttt cggctcgatg
     5281 tcaggtctgt tcgtggcaca ggaaaatcag ggcgacctgt ctgcacttgg cgcaagacgt
     5341 tgctgttctc cagtgaacaa gcttacaagc ataacggcaa cgctccttgc ttttcatgtg
     5401 gtggaaatga tatgcatggt gatatcggtg acgttcctga gatttgtgct taaagtggat
     5461 ttcggcagta agctccccct tgtttacctt gccgcagtgc taggcggaat aatgggaata
     5521 tccatgggat ttttcgttgg ctctttccgc ataaaagagg gtttaaaaat gtctgtagtc
     5581 atggcggtat caatgacctg ctgtttcttc agcggtctta tgtcaaacac catgaaaggc
     5641 actgttgcag agcattgccc gatattcaat gaaataaacc ctgccgcagt tatctcagac
     5701 agcttttact gtcttaatct ctacgaagat tacaggcggt tcacagtgaa gataatttca
     5761 atggcgatat acacagtgct gttcaccctt ggtggatatg ttcttacaag gaggcgtaaa
     5821 tatgcaagtc tttaagacct ttatgaaaat actcaaaaca aggcttacgt ctgctatatt
     5881 atatctcgtc atatttatgg tcatagcagt aataatggcg gatactgcaa aagataacaa
     5941 tgactatgag gactataaaa tgtccatatc cgtcattgac cgtgacaaca gtgccgaaag
     6001 caagcgtttg caggacttta tattcagcgg caacaaaaag gtggagcttg cagatgatga
     6061 agacgaggtc atcgatgccg tttattatca gcgtgccaac tatgttctgt atataaacaa
     6121 gggctttggc gacaagataa acgcagggga ttttgacgga ctttttgaga atttcaaaat
     6181 gccctccagc tacggcggac agctttttga aagcaggctt gacaactatc tttcaagcgt
     6241 aaaggcgtac atgacggcag gtgaaagcac agaaaacgct atggagcttg ccaaaacggc
     6301 agtttctcag caagtaaagg cggagcttaa aaactttaac aacaagggcg gaacaggtat
     6361 gccggcaata ttcggctact attttcagta tcttgcatac gttatgcttt ccatgctcat
     6421 tgttaccctt tgccccgtta tactgacgct aaacagaaaa ggcgttcgag aacgcacaat
     6481 gtgttcaagc ctgacgtcag caaactacag cagacagacg gctcttgggg caagtatctt
     6541 ggtgtttagc atatggcttt tgtttatgat agtcgcaatt atttggggca aaacgctcag
     6601 cgtgaaattc tggcttgcct gccttaacag ctttgtatat atcattgccg ccgcaggcat
     6661 agcaatgata atagcgcagt ttgaccttag cgacaacggc ataactgcta tatcgaacat
     6721 aatcggtctt ggcatgagct ttctttgcgg agttttcgtg ccgcaggaat tgctcacagg
     6781 aggcgttctt gcagtgggaa gattgttccc tgcatattgg tacaccaaag ccaacaatat
     6841 gcttttcggc atgagcggtg gagtattcag cacaaaggaa tatcttatct gcattggtat
     6901 cgaagcactg tttgcgatag cgttttttgc tgtggctgct ctgcttgtta agcaaaagcg
     6961 tgagggtgat gtaaattccc ttgcataagt tgacaataat atggctgtgt gttatactct
     7021 tattgttacg gagctgacac gcagcctttt tgcgtcagct aaagaagatc atagatgaaa
     7081 aaagaggaaa aagtatggat agattacagc ttgacaaaaa tgcaaagctc acaggcagag
     7141 tatatttcaa agacgaggta atgtggctcg gtctttcagg aagcggtgcg tcattcacat
     7201 ttgacggcac aaagcttgtt ttgaagctca aaggcgacaa tatcgcagtg ggcgataatc
     7261 ctatcgacta tgcaaggata gccgtttatg ttgacggcaa gagagtagtt gacgagatga
     7321 tcgactgtgc agaaaaagag atcacggtaa tagaccgcac ggaaaacgct cagtcagttg
     7381 tggagataat aaagctttca gaatctccaa tgtcggcagt tggcgttgag gctttcaccg
     7441 agggcagtgc tgaggctttg cctgaaaaac cgctcaaaat agaatttatc ggcgactcta
     7501 taacctgcgg ctatggtgtt gatgatgagg acgaaaacca ccagttcacc accgccacag
     7561 aggacgttac aaaagcctac gcctacaaga ccgccaaggc acttgatgct gaatacagta
     7621 tgttttcagc cagcggctat ggcatagtat caggctacac agaaggtgac gagcctatca
     7681 gtgaacagac aattccacag ttctattgca gctacggctt ttcaaggggc aattttgcag
     7741 gaactgctcc gcaggatatc ccatgggatt tcagcaaata tgttccgcag ataatcgtta
     7801 taaacctcgg cacaaacgac gacagctact gccgtgacta caaggacagg caggctgttt
     7861 tctcaaagct ttatgctgat tttcttgcgg tcgttcgtga gaaaaatccg caggcttaca
     7921 ttctctgcac tgtcggaata atgggcgaga ggatattccc tgctgttgaa gagggaataa
     7981 agatatatct taaaagccac gctgacagca agataagctc aatgatgttc catgagcagg
     8041 acggcgcaaa cgatggcttt gcggcaaact ggcaccctac agaaaagacc cacaacaagg
     8101 cagctgcggt gcttacggaa aaactcagag agataataaa aagcatataa caaaagcggc
     8161 agtcggtaac ggctgccgct tttttgtcat tttacgccaa ttctcattct gtagcagaac
     8221 gaacaggaaa gcatggctag tttcagacct gttctttgaa agctttcctt ataagaaaac
     8281 atatttcagc acgaaaagca ctgcaagcac atacataagc ggtgtgatat ccttgcgctt
     8341 tccacaaaca agatttataa caacgtatga gattattcca agagagatgc cctctgaaat
     8401 gctgtagaaa agaggcattg aaagaataca aagatacgca gggatagcct ttgtataatt
     8461 gccgtcatca aacttgatgt cagtgataga gctgaacatc aaaaagccta ctactataag
     8521 tgcaggagct gttgcaaagg atggtatagc cgtgaagata ggtgcaaaga acattgacag
     8581 tgcaaacagc actgcggctg tgatagaggt aagacctgtt ctgccgcctg cggcaacgcc
     8641 tgctgaggac tcaacgaatg ttgtggtggt ggaagtacca agaactgcgc ctgccgttgt
     8701 ggcaacggcg tcagcaagaa gcgcaggctt tatctgcgga agtctgccct tttcatcaag
     8761 catatctgcc tttgaacata cgccgataag agtgcctaaa gtgtcgaaaa ggtcaacgaa
     8821 caggaaggag aataccacga ctataaaatt gaagatacca atgctgtgca tatccacctt
     8881 aaagcactga ccaaatgttt cgccaagctt tgaaaaatcc gttagcgaca ttgtaggaaa
     8941 aagcgaatta tagccgcttt cagcgtcagg aacatacagc cctacaagtt gacatatcat
     9001 gcctattacc catgttgcca ctatacccat aagaatagag ccacggactt ttttgatata
     9061 taggataata gtgatgaaaa gtcccacgat agtgagaagc gcacatatgc ccgaagttgt
     9121 gaaattcttg cggaagcctg ttatggtaac aagagtggct gaactgtcaa cgcaaagtcc
     9181 agcattctga agtccgataa aggcaatgta aagaccaata ccaacggaca ccgctttttt
     9241 cagcaccaca ggtattgcat tgaaaatggc ttctctgacg tttgtaagag aaagcacgat
     9301 aaagacgata ccctcgacga aaaccgccag cagtgcaacc tgccatggat agccgtagcc
     9361 tgccacaaca gtgtaagcca tgtaagcgtt aagtcccatg cctgcggaaa gcacaaaagg
     9421 cagatttgcc ataagtccca tgcacactgt tccaataaca gaggcgatac aggtggcaag
     9481 aagtacggct gtaggatcca tgcccgtact gccgagaatg ctcgggttta tggcaaggat
     9541 atatgccatg gtcatgaaag tcgtcacgcc cgccattatc tcagttttaa catcggtgtg
     9601 attttctttc agcttgaata ttttttccgt catattttat tatgcctgcg ttgcagacat
     9661 ttcctccttt caatatgggg atactatctt attataccga ttttacagca tttttcaagc
     9721 ctgatttatt aaggcaacac cgcacaaagc ccgcctgacg gctttgcacg ccatctgctt
     9781 gca
//
LOCUS       DAWEMR010000094         9770 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2908_length_9770_cov_11.271436, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000094 DAWEMR010000000
VERSION     DAWEMR010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9770)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2908_length_9770_cov_11.271436"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4707..4716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6381..6390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3024 a         1877 c         2340 g         2509 t
ORIGIN      
        1 ctttacataa tgggtacgag aagtgcagct tctatcgctt cgttccttgc ttattatctt
       61 ggacttattt tcccacatgt aaagcttgtt cagagtacga ctacttccga gctttttgaa
      121 aagataatga gaatagacga aaaggacgcc atgataggca tatctttccc gaggtattca
      181 aagcagactg tcaaggcttt gaagtatgca aagagcaacg gtgcgaacgt tatagctata
      241 acagactcaa aggcttcgcc tatcgcaaag tatgcagaca gcctgcttct tgcaagaagc
      301 gatatggctt cgttcgttga ctctctcgtt gcaccgctga gccttataaa tgcacttatc
      361 gttgcggtat ccataagaaa tatcgacaga gtgtcccaga cctttgaacg tcttgaaaag
      421 gtatgggctg agtatgatgt ttacgcaaaa agcgaggaag atacacagtg agaagatttg
      481 acgcagttat agcaggcgca ggtgcggcag gaatgttctg cgcagttcag cttggctggc
      541 gtggaaagag cgttgctgtt atcgagcata gcggctgtca gggcagaaaa ctcatgataa
      601 caggcaaggg caggtgtaac gtcacaaaca actgtaccgc tgacactgtt atgaaaaata
      661 tcccacgcaa cagcaagttc atgtattcag cactgagcag atttgcacct gaggacgtta
      721 tgagcttttt tgagtgtctt ggtgtggagc ttaaaactga gcgtggcaac agggtgttcc
      781 ctgtgagcga caacgcaaag gatatcgtaa aagcacttgt gaaagcctgt gaggacagtg
      841 gtgtgcagtt tatcgacgat gaggtaaaag agcttatcat aaaggatggc agagcagagg
      901 gctttaaatg cgagcatggg gattattacg cagacagcgt tatcgttgca actggcggaa
      961 agtcctatcc acggacaggc tcaacaggcg acggctataa gcttgccaag tctgtggggc
     1021 ataaggtcac ggcaataact ccgtcacttt gccctgtagt cacttatgag cgtgaggaat
     1081 gtgcttctgc aatggggctt tcccttagaa actgcacctt gacgcttcgt gaagaggaca
     1141 gagataaacc acttttccac gagcttgggg aaatgctttt cacacacttt ggtctgagcg
     1201 gaccgcttgt actttcggct tcggctcacc ttggtgatat gaacaagtca aggtattata
     1261 tggatatcga ccttaaacct gcgctgagcc atgaacagct tgacagcagg atacttcgtg
     1321 atttcggtga ttttccaaac agagattttc agaattcact gggtaagctg ctgccctcga
     1381 agataatacc ccttataata aaaaggtgcg gcatcgatcc tgagaaaaag gtcaatcaga
     1441 taacccgtga ggagcgtgaa aggcttgttt ccacagtgaa aaagctaacg ttcacggtga
     1501 agtgtatgcg ccctgtagag gaagctatca tcacccgtgg aggagttgat gtttcgcaga
     1561 tagacccacg gtcaatggag tcaaagctgt gtaaaggact ttactttatc ggtgaggtgc
     1621 ttgacgttga cgcttataca gggggtttta atttgcagat agcgttttcc acggcgtata
     1681 gctgtgcgga taaaatagga taaggagatt ttgatatggg aataaacgtg gctctcgacg
     1741 gaccttccgg agcaggaaag tccactatcg caaaggcggt agcgaaaaag cttgaatatg
     1801 tatatgtaga cacaggtgcg atgtaccgct ctatagctta ttatgttata agcaagggtg
     1861 cagacctttc agaccctgag cagataaaac cccttcttgg tgagatatcc ataaagcttt
     1921 gctacacaga agcagggcag agagtaattc tcaatgacga ggacgtttca gacaaaataa
     1981 gaacgcctga gatatccatg ggtgcgtcaa aggtgtctgc tatacctgag gtaagagaat
     2041 ttctgcttga acttcagaag aatatcgcaa aagaaaacaa catcatcatg gacggcagag
     2101 atataggcac tgtggtattg ccaaacgctg acgtgaagat attcctcaca gccgcaccgg
     2161 aagccagagc agaaagacgt tttaaggagc ttcaggaaaa gggcgacaag tctacatatg
     2221 atgaggtatt gcaggatata atccagcgtg actataacga tacacacaga gagatagcac
     2281 cgctgaaaaa ggctgatgat gctgttgagg tggactctac tgagttgact cttgaagagt
     2341 ctgttgaggc tatctacaag gtaataaccg acaagactaa aaaaaaagag cgtaagataa
     2401 aagagataat gcctgtcaga cctgtgaaaa aagaacacag gcttggtcat ttccatatgt
     2461 tttggtacac agtgctgaga tatatcgtta tcggacttta tcatctgtat tttaacatca
     2521 cttttgaggg tacggaaaat atcccaaagg acggcggaaa tatattcgct tcaaaccaca
     2581 gaagctatca ggaccctgtt tttatcgcac tgccaacaaa agtgccgctg tcttatatgg
     2641 ctaaggagga gctttttcat cagaatgtgt tcttcactct gcttataaaa gcgttcggag
     2701 cattccctgt tacaagagga aagggcgaca ctcaggtaat agatacatct atcgaaaagc
     2761 ttgaaaaggg cagaaacctt gtcatcttcc ctgagggaac acgttcaaag gacggcaagg
     2821 taggaaaggg caaaacaggc gttgcattgg tggcggctgt ggctcaggtg cctgtggtcc
     2881 ctgttggtat caatttcgag ggcaagctca aattcagaaa gcgtgtagtt gtaagatttg
     2941 gcaagcctat agtgcctggc gagataggag ttacagccac cgaccctcat tctcttagga
     3001 ctatcaagaa cgagatcatg aaaaatatca cggagttggt acattaaatg ttaacaaagt
     3061 gtaaaatttg cgttgcaaag accgcaggtt tctgttttgg tgtagacaga gccgtaaaaa
     3121 tagtgtataa tgaattagat aaccgaaata atgttgtaac gttaggccca atcattcaca
     3181 atccaaacgt agtttctgac cttgaagcaa aaggagttta ctccaccgat gtggataagg
     3241 tcacgaaaga ccagacagtt gttatcagat cccacggtgt tggactggat gtgtatgaaa
     3301 agcttgcaaa ggtaggtgcg gaagtaattg acgctacctg tccctttgtg gcaaggatac
     3361 acaagatagc agccgagaaa tcgggcgagg gatacgttat tcttatcgca ggtgatgagg
     3421 ctcacccgga aataatgggt ataagaggtc attgcagcgg agagagttat gtgttcagtt
     3481 catgtgacga ttttgaaaat ttggtgaaag agaaagattt ttcgtcaaaa aaggttgcaa
     3541 ttctggctca gacaacgtat aataagaata tgtggagaaa gtgcgaagag ctttttgaga
     3601 ggtatctgcc tgaggctgtg gtgtataaca ccatatgcag tgccacttct gaaagacaga
     3661 aggaggcggc ggagcttgcg aaggctgccg acataatgat aatagtcgga gggctgcaca
     3721 gctccaatac tcacaagctt aaagccatat gcgatgaata ttgcaaatgt tggcttgtgg
     3781 aggacgccga aggactgagg gcatgtgata ttgacttatc aggtgcaaaa tttatcggga
     3841 tttctgctgg tgcatcgacg ccggcttata taatcaagga ggtacaacag accatgagtg
     3901 aaatgttgaa caatgttgac gaagaattta attttgagga ggaactggaa aagactctga
     3961 aaaaaataca tacgggaatg aaggttgagg gcattgtcac cgatatcaat aacggtgaag
     4021 tagccgtaga tatcggaaca aagcacacag gctatattcc tgcatctgaa cttacagatg
     4081 atcctactaa gaagccagag gatatcgtaa aggtaggcga caagatcgat ctgatcgttc
     4141 ttaagacaaa tgaccaggag ggtatcgtta cactttctaa gaagaaggtt gacgcagttc
     4201 ttggtttcca gaagatagtt gaggctaagg aagctgacgc tacacttaca ggtacagtta
     4261 caaacgttgt taagggcggt gtactcgtat cagctaacgg tgttaaggta tttatccctg
     4321 cttcacaggc agctccaaga agagatttcg atcttaacga tctgctcaag cagtcagtaa
     4381 gcttcaagat ccttgaggta aacgaggcta agcagagagc agttggttct atccgtgcag
     4441 tagcaagaga ggaaagagca gcagctcagg ctaagttctt tgagacagct cagatcggtt
     4501 ctgaggtaga gggtacagtt aagtctatca cagattacgg cgtattcgta gatctcggtg
     4561 gtgttgacgg ccttatcaga agaatggatc tttcttggaa caggatcaag cacccttcag
     4621 acgttgtttc tgttggcgat aagatcactg ttactatcaa ggatatcgac agcgaaacaa
     4681 agaaggtttc attgacatat aagaagnnnn nnnnnnaact atgaggtagg tcaggtagtt
     4741 aaggctactg ttgtttctat cacaagcttt ggtgcttttg cacagatcat tgacggtatc
     4801 gacggtctta tccatatctc tcagatcgct aaccagagag taaacaatgt tgcagatatc
     4861 ctttctgtag gtcaggtagt tgattgccag atcacagaga tagatctcga caagaagagg
     4921 atcagcctgt ctatgagagc tttgcttcct gctgatgacg aggcttctga ggacgctgaa
     4981 taagcaataa aattcatggg ggcgagtgat cgccctcgtt ttttattttc aatgaaataa
     5041 cggcagcttt gttctgtcaa aaaatcctta gaaaaaacta aagatttacc gaaaagactt
     5101 aactttttga ttcgtatgtg gtataataat atcagatcaa ataaagacga aaggttaaat
     5161 ttatggatga aataaaaaac acggcagtta aaaaacgcaa aaagaaaaga tccactaatc
     5221 ctgctgtcag agttctcaaa atattaggca cagctttgct gtcgatattc ctgatactca
     5281 ttatcacctg ttctatattt gcaacagttc tgactattta cgtccttaac tttgcagata
     5341 ccacaacaac tatcagcctt gacaagacgg aaacaagcaa catttcgaga tttttgtctg
     5401 tcaaccctga ctatgatgaa gatgatgagg attcacagga gtatgacctt tattatgctc
     5461 tgaaaaacag caataagcac gttgtatggg ctgatcttga ggatattcct cagtatgttc
     5521 aggacgcttt tgtgtacacc gaggacgaga gattttattc tcatgacggc gttgacttca
     5581 agagaacgtt cgcttcattt gtcaatgtat tcataccgat atacggcggc agacagatag
     5641 gtggttcgac tatcactcag cagactatca agaacatcac gggagatgac tccagagatt
     5701 ctatccatgg tatcgagcgt aagatacgag agatcttccg ttctatcaac gtggaaaaga
     5761 cctacacaaa ggaagatatc ctccaaagct atcttaacct tgtgccactg acaactcagg
     5821 agtatgatat catcggagtt caggctgctg caaacttcta ttttggcaag gacgtaaagg
     5881 atcttaacct tgctgaggca gcttcacttg cgggtatgac aagctggcct gctgctaaca
     5941 acccatatga taatatgaaa aacaacaagc tcagacagaa gtatacactg gatcatatgc
     6001 tcgataacgg tgcaatttct gagcaggaat ataacgaagc cctcaactat gagcttaaaa
     6061 taacaggaga tataacctac acaagctcaa ctatctatga ggacgaaaca aaggatcagg
     6121 gtccgacatc ttatttcatg gacgctgcta tcaatcagac tatacagata atcgctgact
     6181 attatggcat ttcatgggag gacgcttccg caagacttta tgacggcgga tttactgcat
     6241 ataccactgt tgacagaagt atgcagaaga aggtggaaaa ggaaatgcaa aagcaaagca
     6301 atttcactac ctatgaaatg aacaaaaagg acgatactct gtggtcaggc tttatcgcca
     6361 tggactacca gggcaatgtt nnnnnnnnnn ggcaatgtta aggctatcgt tggcggacgt
     6421 gataagaaag aggagtcgag agtttataat attgctactg atgccaaacg ttcaccaggt
     6481 tcttgtatca agcctattgc gtcatacgct ccagcgctgg atcaggacct tatgacatgg
     6541 tcaaccctgt tcactgatga gcctatcact attaaagtaa agggcaagga caaaaagtgg
     6601 cctgtaaact attctgaaac aggcgacagt gcaaactggt cttatcagca gcttacaact
     6661 gtagaaatgc tcacaaggtc tcttaatacg cttcctgcac agttgataaa gaaaatgaag
     6721 cctgtttatt cctataactt cctcaaggaa aagcttgaca tcactacgct tgcagactcc
     6781 gacgctgact attcaccagt aactgtcggc ggtcttacaa atggtactaa gcttgaggag
     6841 cttgtaggcg cttatatgat attcggcaat ggcggtaaga agtatgacgt tacctacgtt
     6901 tcaaaggtag aggacgctga cggaaacgct atctatgaaa agagcgacgg atacaagcag
     6961 gctatcagcg agtctacagc ttacgttatg aacagaatga tgcagaatgt tataactcag
     7021 caggacggta ctggtagata tgctaagcta aacaatactg accttgtcgg taagacaggt
     7081 acttcatctg actggtttga cctctctttt gtaggctgta ctcctgacta tgtttcaggt
     7141 atctggatag gttatgaaaa ccctgccact atccctacga acgagtatca gaatatcggt
     7201 gcgatttgga agaacgtttt tggtgatatc gctgagaatg agaagcataa gtcatttgac
     7261 aagactttca aaatgcctga tacagttgaa aagctcacct actgcacaaa gacaggtaag
     7321 cttgcgtcaa attcatgcta tagcacggca actggatatt acaagaagtc caacgagcct
     7381 gagtattgca gcggaaatca ttaaaattaa agcctccata actctatgga ggcttttttg
     7441 aaaggaaatc attatggaaa ataaactgcg aatgtgcgtt ccatgccatt tcgggcttga
     7501 aagcgtgctt gcatatgaga taaaaaagat aggcggtgaa aatcttacca caactgacgg
     7561 caagataagc tttgaaggtg gctttgacgt gcttgcaaag gcaaatcttt ggatcgccac
     7621 aggagagcgt gtacttatcg agcttggcag ctttagagca gaaagctttg aacagctttt
     7681 tgacggtgtt atgaatcttc cgctggagaa ctttatcggc agggaggacg cttttcctgt
     7741 taagggtcat gcccttaatt caaagcttca ttctatccct gattgtcaga agataatcaa
     7801 aaaagcctgc gttaaaagac ttgaaaaagc ctatggcata agctattttg aggaaactgc
     7861 agcgaaacat cagatacagt ttgctatcca taaggacatt gcgactatct atcttgatac
     7921 atcaggtgta ggacttcata agcgtggcta cagaagaaat tccaacgacg cacctatcaa
     7981 ggaaacactt gccgcaggca tagttgacct tgcaagagtt cgtgcggact cagttgtggt
     8041 ggatcctatg tgcggttcag gtacatttct tattgagtcg gcatacaagg cactgaaagt
     8101 tgcacctggt ctgagaagaa attttgcggc agagcagtgg tcacagatac ctgaaacagc
     8161 gtggcgcaat gcaagagccg aggctatgga cgctatcgac aggcagggcg cattcaaggc
     8221 atttggcttt gatgttgatg atatagcggt tgagctaaca agaaacaacg ctaaaaaggc
     8281 aggagtaagc tcaaagctta cagtaaaaca gcaggatatc tcaaaataca gccagataga
     8341 gggttcgata acaattgtca atcccccata tggcgagcgt atgcttgaaa taaaggaagc
     8401 cgaggagctt tataagaaaa tgggacaaag actttgcccg tcaaaggaga gcccatgcta
     8461 tataatctcc ccacatgagg agtttgagat gtttttcggc aaaaaggctg acaaaaagcg
     8521 caagctttac aacggcatga taaaatgtaa tatgtatatg tatttcaagt gaggtgcgtg
     8581 gtgggtttga aattatcaga acttgacgga atcgtattcg atatggacgg cgttattttc
     8641 gacagcgaag taataggtct tgaaagctgg gagcgccttg gaaagaaata tggtctgacc
     8701 aacgttcacg aaaatgctat gaagtgcatt ggcagaagca ctgttgacac aatggctatc
     8761 ctagaagcca catatggtga caaagtgtca atacccgaac tttatgacga agcaaaagcc
     8821 atttgtgcag atattatcgc tgagcgtggt ttgcctctga aaagcggagc aagggagttg
     8881 ctttcttatc ttaaagagcg tgatataaag gtaggacttg cttcatcaac gtcttacaaa
     8941 ggggttatca aaaatctaaa gggagcaggt ctgcttgatt atttccatgt ggttattggc
     9001 ggagatatga taaaacattc aaagccacag cctgagatat atttacttgc ctgtgagaag
     9061 ctgggagcaa ggcctgagaa aactgttgct gtagaggact cttacaatgg cataaaggcg
     9121 gcttacaatg cgaagatgat accaataatg gtgccagatc ttattgcacc gacagatgag
     9181 atattgtcaa tggtgtacaa gaaaatggac agtttggacg ctgtccttga atattttaaa
     9241 gcataaacaa agcccctcgg cttatcatcg aggggctttg ttttaagtat gatgaaataa
     9301 atgcagtggt atcattttaa aaatagcata agtcgatcgg tcaggtgttc tgatcagttc
     9361 aagagaatgc ttttttcgta cccgtcaaac cctctgcacc ctactctaaa aaacagactc
     9421 tgcgagggat caccattcca gcaggagcga atagactttt ctttggcaaa cccttacgaa
     9481 aagaaacacc gccctactct accaaagtaa cggtgtttct tttcactaaa tctttgggag
     9541 aaaccgctta gccaataggc ttatgcagta cccctttata tatcagtata gcataagtca
     9601 aaagaaaagt caatcttatg ctatcaactg caaaaaatct aaatatatcg acattttatg
     9661 cagttaacat acataagtca ccataattac gataaaaaat tacaataaaa aattataaat
     9721 agtacaaaat cattgacaat ttatgtgaaa tatgatatcc gtatattgtt
//
LOCUS       DAWEMR010000095         9732 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_2924_length_9732_cov_10.178981, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000095 DAWEMR010000000
VERSION     DAWEMR010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2924_length_9732_cov_10.178981"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3614..3623
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2707 a         1945 c         2323 g         2747 t
ORIGIN      
        1 tcttttccaa atcagttgac aatcttttgt taatatgata taattatttt gttaattttt
       61 tgtcgattta tcagttaatc tgaggtgtat taataaatgg ataatctttc taatataagt
      121 gtgataaaag atgttctcgc aagacacaat ttcactttct ccaaggctct cggtcagaat
      181 ttcttagtga atccctccgt ttgccccaag atagccgaaa tgggcaatgc aaaaaaaggc
      241 ttcggcataa tcgagatagg cacaggcata ggcgttctca caaacgagct tgccaagcgt
      301 gctgacaagg tggtagccat agaaattgac gacaggctta tgccggtcct tgcagaaact
      361 cttgcagaat atgacaatgt gaagataata aatgccgatg tcatggaaac tgacctccat
      421 aagcttatcg ccgaggaatt tcagggcatg gacgttgccg tttgtgcaaa tctgccttac
      481 tatataacct cgccaatatt aatgatgctc cttgaagaac gtctgcccat aaagtctgtt
      541 actgtcatgg tgcagaaaga agcaggcaca cgtctttgtg cccctatggg tacaagagat
      601 atgggagcag tcacagtggc aataaattat ttttctgtac cgaaactgct tttcaatgta
      661 tcacgaggaa gcttcatgcc ctcgcctaat gttgacagtt gcgtggtgag gtttgatatc
      721 cgtgataaga cccccgaggg cgtgacggac gaaaagttct tttttaaagt cgctagggga
      781 gcattttcac agcgaagaaa gaccctctca aattccgtat catcatcaat gggcatagat
      841 aaatctatag taaatcaggc gatagagcaa agcaatctgc ctgcctccgt ccgtcctgaa
      901 gctctttcca tggaacaatt cataaccttt tccgaaaccc ttagaaagct tatctaattg
      961 tacatatttt cgtatagtta tacttcatta cttgtagagg agcgtctcag tttctgcaag
     1021 cagaaatgcg acgtcccatt tcgttcgccc gttgctcggc aactatcccg aatttcaaca
     1081 gcagattttc atgacgttga aagttacgag atctgtccgt aaatttggtc agtatttcaa
     1141 tttcaagccc atattgcgac acctttcgct tgatagtcct gctaaaatgt aattattaca
     1201 ttttggaaac ataggacgtg ataagcaatg gaaaaaatac ggcatacggc aaaatttcat
     1261 acctcaggca aaacgaatgt acttatggct gttttatcgg cggcagcagc gtttacagca
     1321 agcttcggaa agatcttagg cttcccctcg tcaatgaacg tggcggtggc agtgctttcg
     1381 ggaactaatg tgatacctgc ttttttaggc tctgcacttg catattttgt aagcggaaca
     1441 ttctcagagg gtatcgttca gctttgtgcg atacttgtta taggggcggt aaggcttgtg
     1501 atgccatctg ctgaccataa ggacgacccc gtttttgtgt cgctcctcac aacaggatca
     1561 atgcttcttt tcagctgtgt aatgtcggtg gcaatgccat cggatacata tacggcttca
     1621 ctgcgaatga taagctctct catgtgtggc tgtgttgttt ttattgcact gaccgtaaaa
     1681 cgtcaaaaaa accgaagcgg tgtttttgac cttacaggaa taaacggagt tttcacagct
     1741 atactttata ttatgtttat ctcgaccatc actgccgcac cgctctgtgt ggtgaacctg
     1801 ggcaggatag caggcacttt gtttatgctt atggcagtgc gaaagtatag aaatataggc
     1861 ggcgcagttg ttggtgcatt gaccacctgc ggtgtactcc tttgtactcc gtcacttgcg
     1921 agaaatacac ttttgcttgc cacgtcagga cttatctgcg gagcttttct gcaattcggc
     1981 tctcttgtga tagtgcttgt tttccttgca gtttcacttg tgagccttgt ggcaatgggc
     2041 gtgaatggtg atacattttc aatgtttgct gatctgctta taggctctgt gctttttatc
     2101 gccctgcctg ttcctgtgat aaaaagggtg gcaaaaaaag tggtgggagt gaaaaattcc
     2161 gttgaccttg tagggcagac cacttcttca aagctcaata tggcttcgag aactctgggc
     2221 ggtatcagag gtcagcttac aatggtcact gcggcgatag ataaacgctc aaaggaagct
     2281 acactgtcaa agcgtgtacg atctgttgtt tgtaccgatt gcgatatgta ctcagtgtgc
     2341 agtaagcgtt gtgtacagct tgatgacagc ttttcaaagc ttgaaagcat agtttgtaca
     2401 tataataacc tcagccttga cgacgttgaa aagcatttgg gaggctgtgt gagaaagtcg
     2461 cttctttgca aaagcttcaa tgaattgtat accgactttg tggcagaacg tgccgacagc
     2521 atgagagttc gtgaaatgcg tgagttttta agcgaacagc tttcctccat ggaggatatc
     2581 ttatccgacc tttctttcag ggcaggtcag gtgagaagcg ttgatcctca gctttcagca
     2641 agagtaaggg agcatttctc aaaccttggc tatccaaatg tgaaagcctg cgtttacata
     2701 gacgaaaacc tttgtcagcg tgtggacgtt ttcataactg cgcagttcag gggcgatctt
     2761 gtaaggctca cagcaacgct ttcagaaatg atagattatg accttgatat gcctgtgata
     2821 gtcaaggtgg ataatataac cagaatgtcc tttgcggaaa taccgaaatt cacagttgat
     2881 ataaaaagct tttcagcttc gtcaagcgga gaatattcgg gggacagctt tgaagtcttc
     2941 gattcaagcg tcaacgaaaa gtatatcgtc ctttcagacg gcatgggaac aggaaaaaga
     3001 gcaagactgg attcattgtt ttccgtaagt cttgttacaa ggctcatacg atcgggaatg
     3061 tctatgcaga cagcccatag gcttataaat tccatgctca gagttaaggg ctgggaggaa
     3121 tcttttgcta cccttgatat aatgcggctg gagcttaatg gcggaacggt gcagtttctg
     3181 aaatcgggtg cgacgcaaag ctttcttttc cgtgacggcg gtctcaaagc tatcggcgga
     3241 caggcttttc ctgctgggat acttgcggag tgtactcctg atgtgagcga gcttaagctc
     3301 tttgacgggg acgttctgct tatgacctca gacggtgtcg aggacgctac agcacgagag
     3361 atacttcgct gtaacggaat gaacgctcag gagatagtca cggcgcttgg cgcaaaggcg
     3421 cttgctcagc gaacaggagg caggcgtgac gatataacta ttattgccgc aaaaattttc
     3481 cttcgtgatg agaatgaaaa atgaatgtaa atgactttgc cgacttttgc ctgcaatttt
     3541 ttacttttgt atactttgac tttacttttt ctatgttatt atataaaatc tattgtgcaa
     3601 aatacataaa accnnnnnnn nnnatctgtt gaaatatgtg cgaatacgag aataactact
     3661 aaaactattg caaatttgtt caaaaaagat taaaatgtaa gtatgaaaca ccgaaattat
     3721 tcagcggttt tcacaaaaag acaagggagg ctgaattatg atagatatta ttccaaagga
     3781 ttatgaactg ccgctctatc ttttccacaa tggcacaaac tgcgaaacat ataagtttct
     3841 tggctgccat aagggagaga aagacggcat agaaggatat tatttcagag tctgggcggc
     3901 acacgctgag ggcatttctg ttgtgggaga tttcaatgac tgggacgaaa atgccactcc
     3961 aatggagaag atagacgact cagaggtgtg ggaggctttt gttacagggc ttaagactta
     4021 tgatacatac aaatactgta tacacggctg tgacggcaag acccattata aggctgatcc
     4081 gtacggcaca cacatggagg tcgctccgaa caccggctca aaggttttcg atatagacga
     4141 ttatctttgg agcgataaaa agtggatgac cgagaagaag tctaaggata tctacaattg
     4201 tcctatgaat atctatgagg tacatctcaa ttcatggaag gcttgtcctg acggaaagta
     4261 tttctcatat gttagatttg cagatactat aatcccttat ataaaggaaa tgggctatac
     4321 tcatatcgag cttatgccgt tggcggaata tccttttgac ggctcttggg gctatcaggg
     4381 tatcggctat tttgcgccga cttcacgttt cggcacgcct tatgacttca tgaaaatgat
     4441 agaccttttc catttggcgg gaataggcgt tatccttgat tgggtgcctg cgcattttcc
     4501 tagagatgaa gcaggattgt tcgagtttga cggaggtccg tcatatgaat actctgaccc
     4561 gaaaaaggca gatcacctca cttggggaac gagagttttc gattattcaa agggcgaggt
     4621 aaagtcattc cttatctcaa acgctcttta ctggatagaa aaatatcacg ttgacggtct
     4681 gagagttgac gctgttgcgt ctatgcttta tcttgactat gacagacgtg acggagagtg
     4741 gacgcctaac aagttcggcg gaaaggaaaa tcttgaagct gttcagttct tcaaggatct
     4801 caataccgct gtgttcggaa gaaaccctaa tacccttatg atagccgaag agtcaacttc
     4861 atggcctatg gtaacaaagc ctgcgagcga cggcggtctt ggcttcaact tcaagtggaa
     4921 tatgggctgg atgaacgata tgctcaaata tatggctctt gaccctattg acagagcttt
     4981 tcatcatgat atgctcacgt tctcattctt ctatgctttc tctgaaaact ttattttgcc
     5041 tatctctcat gacgaggtgg tgcatggtaa ggcttcattg gtaaataaaa tgtatggtgg
     5101 agatatcgac aataagttca agcagtgcaa gctgttcatg gcttatatga tggctcaccc
     5161 aggtaagaag cttctgttca tgggtgctga ttttgctcag ttcagagagt gggattacga
     5221 gaatggtctg gaatggttca tggtggacga atatgagaac caccgcaatt atctgaacta
     5281 ctccaagaac ctcaatcatt tctatcttga ccaccctcag ttctgggaga gagatttctc
     5341 ttgggagggc tttagctgga tatcaaatga cgatttcaag cagagtatca tcatcttcag
     5401 acgttttgac aaaaacggcg gagaggtcat cgtggtttgc aatttcgttc ctgtggaaag
     5461 aaagtcatac tgctttggcg ttccttactg gggcagctat acagaggtgt tcaactcgtc
     5521 ttctgttgat ggatcccctt gcactaacgg tactgtcaag gcggtagatg ttaagatgca
     5581 cggctttgac cagtcagtct gcatcgacat tcctgcgttc tcttgtatgt atttcacggt
     5641 gaagaaagag gaaagacctg tgcctgagaa aaaggcagag aataagaaaa agacaaagac
     5701 ggcggctaag aaaactgctg tcaaaaaagc cgctaaggcg aaaacaacta agaagtccac
     5761 agctaaaaag gctgagtaat atagacttaa taaaaattgg tgggtgaatt aattatgttc
     5821 aacaaaaaag aatgtgttgc aatgctgctg gcaggcggtc agggaagcag attgtatgcc
     5881 ctgacacaga gcgttgcaaa accggcagta cctttcggtg ctaagtatcg tatcattgat
     5941 tttcctctct cgaactgtat caattcaggt atcgacacag tgggcgtgct gacacagtat
     6001 cagccattgg tactcaatga gtacattgga aacggtcagc cttgggatct tgacagaata
     6061 cacggaggcg ttcatgtgct tcctccttat cagacagctt caggcgctga ttggtattcg
     6121 ggtactgcaa acgctattta tcagaatatc gcttttgtag acagatatga ccctgagtat
     6181 gttgttgttc tttcaggcga ccatatctat aaaatggatt acaacaagat gcttgaatat
     6241 cacaaagcta agaacgctga cgctactatc gctgttctgg acgttccaaa ggaagaggcg
     6301 tcacgtttcg gtatcatgat aaccgacgag gacgacaaca tcatagactt cgaggagaag
     6361 ccaaagaacc ctcgttcaac tcttgcttca atgggtattt acatattcac ttggaagaag
     6421 ctgaaagctt atcttatcgc taatgagaac gacaaggaag cttcaaagga cttcggcaag
     6481 aacattatcc ctgacatgag agaagcaggc gagaagcttg tggcttatcg ttttgacggc
     6541 tactggaagg acgttggaac tatcgactct ctttgggaag caaatatgga ccttatcaat
     6601 cctaatattc ctatcgatct ttatgacaca agctggaaga tatactcaag aaaccctgtt
     6661 atgccacctc agagcatagg aaagaacgct caggtacaga actctatggt aacagagggc
     6721 tgcgttatcg acggttctgt agagttctca atgatctctg acggcgttac tgttgaggag
     6781 ggcgctgaga tactggattc tatccttatg cctggcgcag ttgtcaagaa gggtgcaaag
     6841 gtcgagtatg ctatcgttgg cgagaacact gttatcggtg agaacgctca gatcggtgca
     6901 agacctgaaa ctatcgagga caaggactca tggggcgttg ctgtagtggg caataatctg
     6961 accgtttctg acggcagtgt tgttgcacca aaggcaataa tttacgagaa tatctaagaa
     7021 aggaccgagt aaacatggat gtaaatatgg gtaacgtact gggtcttgtt ttcgctaata
     7081 tgcacgacac gactcttggc gacatgacaa agaacagaac tatgggttct gtaatgttcg
     7141 gcggaagata ccgccttatc gacttcccac tgtctaacat ggtaaacagc ggcataagtg
     7201 aggtcggtgt tataacaaaa tctaactatc agtcacttct ggatcacctc ggctctgcaa
     7261 gagagtggga tctggccaga aaaaaaggcg gactttacat tctgcctcct ttcggcaacg
     7321 ttgaaagcac tctttacaga ggcaggatag aggctcttta tggtgcgatg agctttatca
     7381 agcactcaag agctaaatat gttatccttt cagactgcga cgttgttaca aacattgact
     7441 acaagcctat cgttgcggct cacattgaga gcggtgcgga catcactgct gttgcacaca
     7501 ctggcgttta cagctctgat gatataaaga cttcaactgt attcaacgtt gacgctgaca
     7561 agaacgtaac ttctgttctt atcaatcctg acatcagcgg cacttgcaca acgagcctta
     7621 atgtgttcgt tatgtcaatg gatttcctta ttgaaactgt taatgacgca acggctagag
     7681 gaaatgtaag ctttgagaga aacattcttc aggagaagtg cagagagctt aagataaaga
     7741 tctacgagta cgataactat ttcagcaagc tcaacagccc tgaaagctac tttaagtcaa
     7801 acatggctct gcttgaacct gagaatgcac gcaagctgtt cgttccaaag cgttctatct
     7861 acacaaaggt aagcgacaac gctcctgtta agtacgatct ggacagcaag gtatcaaatt
     7921 ctcttattgc tgacggctgt atcatagagg gcgaggtaga gaactctgta ctgttcagag
     7981 gcgttaaggt aggcaagggt gcaaaggtca agaactgtat ccttatgcag ggcactgttg
     8041 ttggtgacaa tgctgagctt aactatctta tcacagacaa gaacgtttct atctgtgaaa
     8101 accatatcct tacaagctcg ccacagtatc ctatgtatgt aggaaaggga gcttctgtat
     8161 aaaaaggtcg caatgactta ggaaagaggg ttcggatatg aacatacttt atactgcaag
     8221 tgaggctttg ccttttattg cttcaggtgg attggctgat gttgcaggct cactgcctgc
     8281 ggctatcaat gccgagggtc acgattgcag agtcgtgata ccttattaca gctctatcag
     8341 gcaggagctt aaggacaagc tgacatatgt tacgaatttc actgtgccgg tggcatggag
     8401 aaatcaatac tgcggcgttt ttacaggcgt tgtgaacgga gtgacttatt atcttttgga
     8461 taatgagtat tacttccaga gaaacggctt gtatggcttt tatgatgatg ctgagagatt
     8521 tatattcttc tcaagggcag ttctcgaact gctgagatac atcgacttta agccaaatgt
     8581 tataaacgcc aatgactggc agacggcact tgttcctgtt tattatgata ttttctaccg
     8641 ctatcagcag ggttatgagg atatcaagac ggtatttacc atacacaata ttcagtatca
     8701 gggacaatat ggcttagaac ttatcaacga tattatgggc attccgcttt atcatacgga
     8761 tctgctttca tatgacggtg atgtgaactt tatgaaggct gctattgaaa cggctgacaa
     8821 ggtgacgaca gtttcgccaa gctatgcttg ggagatactt gatccatggt attcacatgg
     8881 tcttgacagg gagcttgtga acaagcaata caagctttgt ggaatactca acggaataga
     8941 tcagaagatc tatgatcctg caactgatcc tgttattgct aagaattata gtttgaatga
     9001 caagacgggc aaggctgttt gcaagcacgc tttgctcagc gagcttggca tggcacatgg
     9061 aaaagagcct gttatcggta tcgtaacgag atttgtttca cacaagggtc ttgaccttat
     9121 caagtatgtg tttgaggaca tcatcaggct tggctgcaag tttgctattc ttggttcggg
     9181 agagaagata tacgaggact ttttcagcga aatgcattat cgttatcctg acagagtggg
     9241 tctgaccatt ggttttgtgc ctgagctgtc caagaagata tacgctggtg cggatatgtt
     9301 ccttatgccg tcgcagagcg agccttgcgg tttggctcag atgatcgctc tgagatatgg
     9361 tactatacct atcgtaagag agacaggcgg tttgagagac agtatccatg atgcaggcga
     9421 caagaatgga aacggcttta ctttcaagtc ttacaacgca catgatatgc ttgacgctgt
     9481 tgttagagcc agggcatact atgatgacaa gatgaagtgg ggcaggcttg tgaacactgc
     9541 gctcaagcag aatttcagtt ggacaaagtc cgccaaggat tacattgcac tttacaacga
     9601 cattcactaa acaccaatat ccgtttattt tgcctgataa acgggaaaga ccgtccctta
     9661 tcttaggggc ggtctttttg catttatata agtgtacgat caattgtaca tttattcagg
     9721 gatttgtcaa ca
//
LOCUS       DAWEMR010000096         9444 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3027_length_9444_cov_8.885717, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000096 DAWEMR010000000
VERSION     DAWEMR010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3027_length_9444_cov_8.885717"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1283..1292
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3973..4032
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2929 a         1790 c         1989 g         2666 t
ORIGIN      
        1 aaataagtgg aggtgtatct acatattatg aagaaatttt cctatgttgc taaggatacc
       61 tcgggaaaaa ccataaaggg tatctatgag gctgaggatg cacaggaagt actggacaag
      121 atccacgaac aaggtctttt ctgtataagt tatacagaag cattgggcgg tgcaggcaaa
      181 caatctgtac acaagttcaa tactaaggaa cttgctttct gctgccgtca gcttgcggca
      241 atgatgacat cgggtcttac cattgttaaa gcgctcgaca ttctctataa ggaagaggag
      301 aataagggtg caaaggatgt ttggagaagc gtttatgacg atgttcagaa aggtcagagc
      361 tttaccgagg ctcttgatac aagaagagga agctttccgg actttttcat ctcaatggta
      421 gacgcaggtg agacatcagg tacgctcgat atgacaatgc agcgtctgag cgattactat
      481 gcaaactcaa acaagttgaa caataaggta aagagtgcga tgacttatcc tatcatactt
      541 ggcgttcttt gtatcgtcat ggtaatcggt atgtttacgt tcatcatgcc tatatttgca
      601 ggtcttatgt cagaggatca gatgccagca ctttctaagg ctctcttctc attcagtgac
      661 tttttcaagg caaaatggta tatcattctt atcgtggtcg ttgttttgat attcggttgg
      721 atatacgcct tgaaagtacc gtcagtcaga cttaaatttg actacataaa gataaagata
      781 cctgccgccg gaaagcttat ggtaaagatc tacgagggtc gtttcgcaag aactctttca
      841 tcactttatt caagcggtat cccaatggta gagtgtcttg aacgttcgtc aagaatactt
      901 ggaaatgctt atgtcgataa gatgttcatt caggtagttg atgaagttaa gcagggcgca
      961 ccgctttcag cgtcacttgc aaaaacagag atatttgaag gaatgtttac ttccatcatc
     1021 tatgtcggtg aggaatcagg tgctctcgat gagatccttg agaaaactgc tgactactat
     1081 gaggaagaag cagacgcagc cgttacaaga cttgtaggac ttatggaacc attgctcatc
     1141 gtattcatgg gtgttgcgat cggtctttgc cttgcgggta tcttcccact gctctacggt
     1201 tcattgtcag gtctggaaaa ctaagggtct gcattttata ttcgttaatt ttaaatatct
     1261 cctctccttt aaaatggaga ggnnnnnnnn nnatttgata tttctgatag acaaatcaac
     1321 attgttaaag gtgataactc ctctaacaag ataaggatcg agagatcttt gcaggtagac
     1381 attcctgagg atatgatcct taatggtgag gtcatcaacc tttcaggtct gtctgagttg
     1441 ctgctcactc agatcaaggc tgaggacatg atggataagg acgctatcgt tactttttca
     1501 tcgagcaaca tcatattcaa ggagcttgta gttccaaagg caaagggtaa tgagttcctg
     1561 cttatggtac agaaccatat gagccaggaa atgggtatca cagatgagta ttctatctca
     1621 tatacagtag taggtgaagc aggtgaggat aatcctggtg cagtgaaggt acttgcaaca
     1681 gcttgcccta gtacgatagt tgaaagctac agaaagcttt ataacattat gggcatgaac
     1741 ctcagatctg ttaacatcgg atgtaactct atcgcaagaa tcgttcttgc tgacaagtcc
     1801 aacatggaga aaatgccact gttggtttgc cagatcgata agaacttcct tggtcttaca
     1861 ctttttgaga atggacagat ggcattcgcc agatacgttc ctatctcaga agaagattat
     1921 gattctgacg attatatcac tgaagctctt aacgagaaca tcttcagaat ggagcagttc
     1981 aataaggcaa gaggcggttc aggacttgaa aatgttattc tctatggctt tatcgaggac
     2041 tacatcaaac tcgttgacgc tcttgacggt cttgatataa aggcttccgt tctcggtgtg
     2101 cctgctcaga tcacaggttt cgagaacttc gagtttacag tattcgctaa tgctatcggt
     2161 gctctctata agagaaacaa ggcaacagag aagatcaacc tcctcgaagt cgatcagtcc
     2221 acaggtaagg gcggcaacag cggaatgggt tcgctgctca tgacaggcgg tatcttcgct
     2281 gtggcagccg cagtgcttat cgcaggtgca acacttgtta ttaatctcag gaagagttct
     2341 atcgagaaag atatagacaa gactaatgat aagatagctg tttgcaatga gaagatcgcc
     2401 cagaaccaga tacttcacaa taagatgaag atcatgcagg attattcaaa ctatgttaca
     2461 tcagcaaatg cagctcttga aacacttccg gtcgtgtcaa gtgagatgtt tgacgatatc
     2521 aaggaagctc ttggttcttt caagtatgaa ggctttacat ataaccttga agctggtaca
     2581 gtaacactta ctaaggttga gcttgatgat cagtcagatg taaaaacagc aatcgatgcc
     2641 cttgtaggta caggactctt ccaggaagat gtaggataca acggatattc gatcggtgaa
     2701 gacggcagct caggcgtaac gcttgattcg ctgacacttt atcttaaaga agagggcgag
     2761 ggtgaataat tatgaagttg aattatcgtg ataaaatgat tttgatagta gtattcgtac
     2821 tgcttataat tgttgcaggt tttatgcttt ttattaagcc tgccattgat gagtgtagtc
     2881 aggcaagcag tgatctggaa tcagcaaagg tacagctcag cgagcttgaa gatcaggtag
     2941 acaaggataa gaaccttgcc gctgagattc agacacttta tacaagcacc tcacaggtcg
     3001 cagctaactt ctatgattat caggtagcat acaaggctac tgataaggtt cgtgagcttt
     3061 ttaatgttga tgatgtaaag atcaaaaact ccaatatgac tatttcatca tacggcagca
     3121 cagttctttc gccttttgct tatgagtcaa cagcgacagc tactgatttt gacactaagg
     3181 tagatgaata caataatgca agcaaaactg attcatcagt tgcagacgca aacacagatg
     3241 caaatgcagc tgataccaat gaaacagctg cacagacaat aggttactat agccttaaca
     3301 ttcagttcaa aagctcgctt tcaggtttca agaattttgc tgacaacctg acgacaaaca
     3361 acgaaaagag catggtgatc gagaatgtta gcattgaaaa tgtcaacgag agcgagatct
     3421 caggctctat gacactgaat atgtacgttc tgaagaagct tgctgatcct tctgccagct
     3481 aagagaaaac tatttggtag gataaggaat atttcttatc ttgccaatta gtgctatata
     3541 atgattaaaa attttatata agtaatcaac ctgtgtaaaa tgaaaaaagt atagcataag
     3601 tgaaaataaa actaagaaag gtggttaata tgaagaatac tccgaagagg aaacaaagaa
     3661 acaaacccgg agtcgtgctt ttcacagcgg ttgctgtcat gctcatgctg tcaatacttt
     3721 tgactgcaac tgtttctttt gtttcggtaa ataggacaaa aacaaatgat aactataaga
     3781 gcaaacaggc ttatctgaca gcttcatcta ctcttgaaag ctttattaat cagatacaga
     3841 ctgatactgc tccaactaat gatcctactg caaaggctca gcagaaaaag gccatagata
     3901 accttaaaaa gctagcaagt gctaatagcg gaaagggtac aactactaat gtcagctata
     3961 atggatcaaa tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nncagagtat acaaatacta ttgagcttgt tggtaacggc ggcgcaggtt
     4081 acgataacct taacgttatc ggtgatatgg ctggtatcaa taataccact ggtaaggtct
     4141 atagatttac aaacaatacc agtatctatg gttcttatct tatgtatggt tctttggagg
     4201 tttcaactca gcctcttatc atgctcaagc ctagtcttgt agatgaaaag cagggttcaa
     4261 ctgttacaat ttctgaaaac cttgatgttt caaatgaatt ccacattaac tccacaatgg
     4321 caagagcaga cggatataac tatgttaata tcggtcagaa gctttccact tcaggttttg
     4381 atgtcgattt gttctgttgt gaagctaaca tcggcggaaa tgactataca cagtatggta
     4441 acttctacgt ttataagggc gcaggtgctt ataatggcga tgcaacattt ggtgcagctg
     4501 gtcagacgat caatggctct ctctatgtcg agggtgatct taatgtcaca aagtcactta
     4561 aggtaacagg ctctgtatat gttaccggca ctatcacggg taaagataag atcgtctgcc
     4621 aggcttcaaa tattcatgaa ggagctgttc ttagcaaagc aggtcgtgac gcaaagccac
     4681 agatacctgt atctgctgat gcctatgtat attatccgga agatttcttc atgagcagtg
     4741 atactaatgt gacaacgatc tctgatcaat atcaggcttt ctatgatggc tcaaacacta
     4801 aaactttcaa tacctttgca agcgatccaa gctattggaa taatgttgac tacacgctta
     4861 ctgagccaat agaccttaca ggaacaggag ctaagactcc agttacaagc cgctacaaat
     4921 tgagaataac atcaagctgt acatgggcat ctgatctttc gttcaatgat tatggcaatg
     4981 gttcaaggat cctcgttgat gtttctgatt cttcgggtga tatcgttata agacttcaga
     5041 atggtcttaa tcttggagca gaatggtcac ctgtaatagt tgttagaaat cgttcaacta
     5101 taattgatac gactacaggt gacagaaaat acaactgcta ttttgtttca gactcaggca
     5161 gtgctattac acttaatggt atcgactcag taacaggcaa gtcacagcat tcaggctcta
     5221 gtgcttgtaa ctatagcttt aagggactta aggtatttga ttatgatact tacgttcgta
     5281 tgtttgattc ttctgttctc aataatacaa agggtaaccc tggcttacca cagagcggct
     5341 ttgttctcaa ccctacatct gtagatgttg caggctctta cagaccgaaa aacagcagca
     5401 tcatcttcct gcttggtgag aacacaacac ttaacgctac agatcaaagc ttcttccagg
     5461 gctcattcta ttcaccacag gctatcgtta atatcgcaac aagcggtctt ggcggacttg
     5521 tggtaacaga ctcagcaggc gaaaaaatgc ccgttcagtg ctgtgcggtc ggcgtagtta
     5581 tcgcaaacag ctttggtaac gccaatacag cgttctatgt atacactaag ccttcaacta
     5641 cttctgtaat gcagaatgcg aagggcggta aggacgattc agcgttcgga tatactcttg
     5701 acagatatga tcactattaa ttgaaaggag tatactcatg aaaaagaaca gcaaaaataa
     5761 aaagggtatg actcttgtag aggtcatcat agcaatggca cttttgtcag gtatgtcagc
     5821 tatgcttgtt acagttgctg tagcggcaaa aaaacagaat cttgaaaact actaccgcaa
     5881 caatgaaatg tatactcagg cagtaaatgc cgagcagtat aatgataaca agaccgtcaa
     5941 tatgaacgag ttgaaggtaa acaagttctc ttcaagtggc aaaactacaa atgagtttgc
     6001 ccttgaggca gactttggaa gcggtataaa gttcaatact acagcctatg gctttaaagc
     6061 aaagcttaat aatattgatg aagacgcagg ctatcagctc aaatttcttc agggtaagga
     6121 cataaacatt acgcctaatc ctgaaaagag cgtttactgg gtaaagtttt ataacaattc
     6181 aggggcagat atgaatttct atgctcttac gcctgagctt acaggcggaa agttctttga
     6241 tgtaaataat aactcatcag gaaattcact tcttatccct atcggtgacg gtggaaatca
     6301 gcagtttggc gttgtgcttt caggcgtaac aggaagcgat tatttcggct tctcacagtc
     6361 atctgatctt tacagcagca ctccaacagc cgatgcagct gcatttaaga tcacagatgc
     6421 gacctttgac cattatcttg aaaaagatgc gtcaggtaaa gctacaggtt atgtagttat
     6481 ccattatatg ggtggctctg attacaaaaa ccaggctgaa tatgacgatg cacaatcaac
     6541 accataagga gggaggatct tgaaaatgaa taaaaaccgc aagggtttta cccttgttga
     6601 gcttatcgtt gtggtaacga ttttcggcgt gatcctcgga gctatcctta atatgatcaa
     6661 acctgcaaat aatgtttatc atgacgctga tgcaacaatg gaaagcaata tcataggttc
     6721 aggtcttata gactatcttg atgatgagct tagatattcg accaatgttc ttgtgcttaa
     6781 agattatatt ggtgtgcctg atgtttcaaa ctctggtact atcggtgcaa gcggtgtgac
     6841 ctattcaaac tgtatcgtta ttgacaataa taatcttcgt ggctatagtc tgaaaaacta
     6901 ttcaggcaat gacacggata cagccgcaaa gagaatgggt gcaaaaggct gtatcctcaa
     6961 tgtaggtaag gtaaatacag agggcttaaa ctttaataat tctgctgttg caagaggcgt
     7021 tgatttttac gacaattata agtttgatat cggtgcaagt atcagcaaaa ttgaggaaat
     7081 gtatactctc gacgtttctc ttacagccta tcagccaaca tatgagaacg gaagctatac
     7141 ttttacgaaa acaaagtata aaaaggatgc agcagtaaat ctcacaaata ttaacataga
     7201 tgaaggcgat tcatacaatg ttaatgacta taaagatttc tctgtagctc ctgattatgt
     7261 cacatatcct caggcaacta ctgctcctgc aggttgcaca gctcagcagg aaaagtatta
     7321 tggttttgat gcttcaaaca catatactta tattttctat gataagacaa cagtttcaag
     7381 ttcaaaaaca tacagtgtaa agtttatcta ctctgcaagc gaccctgaac caacgcttcg
     7441 tggtaaacag atagatacta agtctgttaa ggcgggtaca gtttataaag ctccaccttc
     7501 tatgtcatca agaacaggtt atggcacgcc ctattgggta gacagcaaga ataacgttgc
     7561 tgattttaca acaggcgtaa caataaataa ggatatggta ttctcctgtg tttatccgcc
     7621 tgttgcaccg aaagcccaat ttaatgtaac gtttgagaat atcaacggtt ctacatttac
     7681 aacaacaaaa gtctatgatg gcgactttgc aaatgacccg ggaattccaa ctgacatgga
     7741 tactataaaa caggattttg ttaagtgggt atacaaatca gataactcaa agggtcttac
     7801 tgatgtttct attacagata gctcagttgt tttcgttcca gttgtacaga acaagcataa
     7861 ggttgagttt aaattaaatg gttctttaat aaatgcctca actatttatg tttctgatgg
     7921 acagtatgca aattatccag gagctacccc tgtttcgtca gacgctaata agatatttgg
     7981 cggttgggtc gtagaaggaa caagcgatga tatatctact aaggttataa catcagatac
     8041 tgtttttgag gcaaccttca tttcaaaacc gacaaatgat ttgtacgtta tcagcagtat
     8101 cgcaagaaaa atcaatgatg gtgagataga ctatgatata acaatacaga ataatggttc
     8161 tgatgttgta aagatctggt ctttaagtgc taatgttggt tttgcatttg atcagatgca
     8221 ggcggattgg agattgaaaa ttccaaatga taaggtttgt ggatttacaa cgaacaacga
     8281 tttgaataac ccatcaaact gtgtatttat tccagcgggt ggaaccgtca cagttacgtt
     8341 gtattttaag aagtacaacg atccaaagta tactgaggat ctttcaaagt attcacttaa
     8401 cccatctgat attacagtat ctaaggtaca gtaatttctt ttctcaatca gacccctcca
     8461 cgggtctgat tgctgaaaaa atccccttaa cagaaaactt ctattaagag gattttttga
     8521 gcaaaaaaat tccgcagtat caaactgcgg aatttgtgca attatcttat tatcagcatt
     8581 gcttcgttca tcatctcgtt aagaccagta aagtccatgt accatgagcc gatctgctct
     8641 gagtacaaag ctgcaacagc aatccctatg gagagatatg gaccaaatgc aaatactgaa
     8701 tcaccgctgc ggtgcttctg gataagtcca ccgatcgagc ctgttatcag tccgataaga
     8761 agcgctacca aagcaccctg cattccgaga aacagccctg cggctgccat gagatataca
     8821 tctcccatgc ccattgcttt ctcatgtgtg ataagacaaa taataagcaa aggcacgctc
     8881 acaatgaaaa atccaatcaa gtgtgattta agcgtaagtc ccgtctgatc tttgcagaag
     8941 atgtattccg caacaccaag caaaccaata aatatcacca catatgtgtt gataagctga
     9001 gtgtcccagt ccatgaagaa tacaacgata agagcagaga ataacagaca gacaagtgct
     9061 gcacgaagcg gattgaaaat tccattaaaa tggtagaata cccataagta gagcagtgcg
     9121 ttcagcgttt caactatagt gtatcttgcc gatatctttg caccgcagct tcgacacttt
     9181 ccacgaagta tgcaccagct tactatcggt atcaggtcac gccgtttgat cggctcaccg
     9241 caggtcatac agtgagaatt tcgcttgata agactttcat ttttcggcaa tcggtatatg
     9301 cagacgttaa gaaaactgcc tatgcacgct ccgaagatga acacaaatac ataaatgatt
     9361 atgtatgata aggttaaatt atccacagtg atcctccttt ggattcatta gttaatatat
     9421 taattttatc atactttcat gaag
//
LOCUS       DAWEMR010000097         9442 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3029_length_9442_cov_10.338340, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000097 DAWEMR010000000
VERSION     DAWEMR010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9442)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9442
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3029_length_9442_cov_10.338340"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2415 a         2137 c         2075 g         2815 t
ORIGIN      
        1 tccatatcga gggaatccac gggctttgtt gcgctttttt tacagcgcct tttttagtca
       61 gcatattttt tctaaatttc gccgtatgga ttttattatc cgcttttcaa gtcttgatat
      121 gtaggattgg gatataccga cttcatcagc gacctgcttc tgggtcattt cttttttgcc
      181 tgaaagccca aatcgcattt tcattatctg cttttcacga gggcagagct tttcgatctc
      241 ctccagaaga agctgtttct caacttcgct ttctatgccc ttgttcacgg tgtcgtcatc
      301 ggtgcctaga atatcggata aaagcagttc attgccgtcc cagtcaacat tcagcggttc
      361 gtctatggat atttcatttt tatactgatt tgattttcta agaaacataa gtatctcgtt
      421 ttctatgcac cgtgaagcat aggtcgcaag cttgatgttt ttatcaggac agaaggtgtt
      481 caccgccttg ataagcccta tagagcctat tgagataaga tcctctatgc ctgtgcctgt
      541 tgactcaaac ttctttgcta tatataccac aagcctgaga ttatgcacga taagcagttc
      601 tcgggctttt ttcgggtctg tttcaagaag ctcaaaggct tttttctctt cctctttgtt
      661 taggggcggc ggtaacgaat ctgcaccgtt taggtaatct atagtttcgg ggtcgtctga
      721 aaacaaacgg ctcaaaaatc tgttcagccc tagagcgagt gttttaagtg tggtcatttg
      781 tattcctcct gtattatccc agcaatgcgg ggttgaatat agcccgtgat tttccgttca
      841 gcgagggctt tatgcccaca tagcacctga cgttgtgtac acggtcttta tcgtcgatta
      901 tctttacgct tcctgcggcg gttatgtgga taattccctc gccgcagaca gtagagcagg
      961 ggataagcct gaaaccgctt agagggtcag gctcgtcacg gtcaaggtca tagtgataga
     1021 aaagcctgtc acagcagaaa accaccactg gcgttcctgt gaagctgtcg caaagcctgt
     1081 tcccactgtc gcaaatggca ggaactttca ctatacagcc accattttcg tatatggctt
     1141 cgtattttgc gaccctgttg aaacaccttt gtgtgatgtt ctcataaaga cagagcagac
     1201 catatgctat accgcaggca agggcaagct taaatagtga gatgtcaaaa tacacagtga
     1261 ggtttctgaa aaaaatcacc ttgctccctg atatctgcca aagggtcacc actatacctg
     1321 tgaaaactgc gtccactgca cagtatatga taaagtatcg aagcagttgc caagggtctt
     1381 tacacccaaa ggctataagc actgtgaata tcgcactgac aagctttatc accacaataa
     1441 gaactgcagt gaaatatcct ctgctgtcta tagccataac aagcgacgaa agtcctccca
     1501 ccgagcaagc tgtcaacagc cgcagcggac ttatgacctt gtgacatatc ctgcctgccg
     1561 cttccagcat gataagcgtc ataatggcgt ttgtcacaag gagtatatct atgtaaattt
     1621 tcatggcgtt cctccctttg atattatgat agcactgctt ggatggaaaa attgtcgcaa
     1681 tggggacaaa aaaagatccg ccttccccaa atgggaaagc ggatatgctt atttgatatc
     1741 aaattagtca gcaaagaaaa ctgagaaagc cttatatgcc atataagtgt ctatcttcga
     1801 ggttatctca aatggtgcgt gcattgaaag cactggtacg ccaacgtcga taacgttcat
     1861 gtcaaggttt gcgatatact gtgcgacagt tcctcctccg ccgccgtcga ccttgccaag
     1921 ctcgcctgtc tgccaaacga tattgtgttc gtcaaagagc tttcttactc tgcccacaaa
     1981 ctcagcagaa gcatcggaag tgcctgactt gcctcttgcg cctgtgaact ttgtggcaac
     2041 aacgccgtag tttatctgtg atgagtttct tatctcgaac ggctctgaga atgttgggtc
     2101 atatgccgca tttacgtctg ctgaaaggca ctcggaagca gagataacgt ttctgccctc
     2161 aagtccgtat acctttgcaa ggtcagcaat gaagtatggc agatatgagc tgttaagtcc
     2221 tgtgttgccg tcgcttcctg tttcttcctt gtctgtgagt atggtcatgc aggtcttttt
     2281 agggtcttta catttgagga tagcctgcaa agctgtgtat gcacaaactc tgtcatcctg
     2341 accataagag cctaccatgc ttctgtcaaa gccaacgtcc tgtgccttga atgccggaac
     2401 agcttcaagc tctgcggaaa cgaaatcgtc ctctacgatg ccgtatttct cgttaaggat
     2461 agccattatg ttgagtttta ccttgtttga aacagcgtca tctctgaatg gtctggagcc
     2521 tatgagcaga ttaagctcct cgcccttgat gagctgtgga gcaggtcttt tcatctgagc
     2581 gtctgcaagg tgcggcagga tatcagttac gcagaaaaca gggtcgtcct tgtcctcgcc
     2641 gatatttact gtaacgcttt cgccgtcagc ctttataaca acgccatgaa gtgacattgg
     2701 aacagttgtc cactgatatt tttttatacc gccgtaatag tgtgtcttga acaggcaaag
     2761 ctccttgtcc tcgtaaagtg ggttctgctt aaggtcaaga cgtggtgagt cgatatgagc
     2821 ggcagcaagt ctgatacctt tttcaagacc gtccttgccc ataactgtaa gtatgatagc
     2881 ctttcctctg tttacaaaat agaacttgtc gccggcgtca tacttcttgc ccttttcata
     2941 aggcttgaag cctgcttttt cagcctgaac cactgcttcg ataacagcct cacgctctgt
     3001 ctttgcagtg ttcagaaagc ttttatagcc ctcgcagaac ttgtctgcct ttttgatttt
     3061 ttcttcgcta aggcgaagtc caccgttttt cttatccgaa agaagctttt ctgcaagctt
     3121 ttcgccctcg gacttttctt tcttcttgtt atccattgta aagtcctcct tttaatgtat
     3181 tcttattgaa actgcgttac aatgggcgga tactgttcgc ccctgcgcat ggtttttctt
     3241 aactgttttc tgtaatatcc ccatgactgc tgagtatatc cttataattg tcataggctt
     3301 tgcgtatctt gcgcacatag tttgaagtct ggtcattttc gtcaggtatg tcgctgactt
     3361 tgaaattgtc agggtcaaca gtgccgtttt ctatccaact gtcaacaaga ttcattccgc
     3421 agtgatatgc tgccgctgtc agctctacag agccgtcata cttttccata agatagctga
     3481 gatagtatgt gccatattgg atattaagtt cagggtcgaa catatcgtca taggtgttgt
     3541 cacgctgatc gtcaaggcga tattttatcc aatcgtatgc gtcctccata agctgcataa
     3601 gacctctggc acccactgat gaaacagcgt ccttattaaa gccgctttcc acttttatta
     3661 cagagaacac aagcacaggg tcgcacttgt acttggtgct gtatcttatt acatactcct
     3721 cataatctgt aggatatgaa aaagagttcg gaatatcctt tgccttgttg attatctgct
     3781 cgtgataaag atatgtgagc cagcctatac cgcacagcag aagtatgact gctatgagta
     3841 cgggaccttt ttttatgtgc tttttctttt tacgcctgct gttttttcca ttagccgcca
     3901 ttatatcttt cctttattgt ttctatgacc tcacaggtct tttggaatgt gtcattcaca
     3961 gagccgttgt tctcaatgac gaaatcgctg tgagccttga aaaactccat agtatgctga
     4021 gaagaaaaac gcttttttgc aagctccgcc gaaatgccgt cccttttggt tatcctttca
     4081 agccttatgc caaagtctgc cacaacgctt acgataacac tgcaaagctt atccgcacct
     4141 gcttcaaaaa gggtcggtgc gtcaagaagt atgaacctgc ttggtgcggc gtttatcttt
     4201 tcgtcaataa gcttgtttat aaaagggtat atggtatcat tgagcatttg aagcttattc
     4261 ttatctgaaa aaactatctc cccgagcttc tgtctgtcaa gagaatagtt ctcgtcaaaa
     4321 cagcttggga aaagctttgc aagctcttta cagcattcgc tatcagcgtt tgtgacttct
     4381 cttgcgacta tatcgcagtt tattatctca aagccatttt ctctgaaagc gtccgaaata
     4441 gttgtcttgc ctgcaccgct ttgacctgta agccctacta ctgttttttt cacagtttca
     4501 ccacctgaaa acctgcggct cttaaaagtc ggttatacac tgcaagcccc acgccgtctt
     4561 tgttaggaca gcgtgcatag actttctttg cacctataat gtcaagttct ctgagaacgt
     4621 caaaaagctc tttggcttgt tcctcgtcat ttgcgccata gcagatgtgg ggtatatcaa
     4681 gcccctcgca atcgctgtca gtgaacacca taagagtggt gtcattgtca gcgtgagttt
     4741 tcacatattc tctgaaagcc tcaagactgc tgtcaatgat agtcacatca gcctttgggg
     4801 catagtgctt gtatttcata ccaggggagc ggacttttac attctcaccc agcttttcca
     4861 aaacgccctt gtcgataaga acgttcgggg tgatctccat aagatcctcg gctgagataa
     4921 atcccggtct gagaagtctt attccgtcct cgccttcaaa gcttataaca gttgattcaa
     4981 cacccactcc gcaagctccg ccgtctatta tggcaggtat cctgccgtgc atatcgttaa
     5041 ggacgtgctg tgctgttgta ggcgacgggc ttcctgaaag gtttgccgac ggtgcggcga
     5101 tagggaagcc acaggctgag ataagcgctt ttgccacctt atgtgacggc attcttatgc
     5161 ccactgtgtc aagtcctccg ctcgttacca ttggtatttt ttcagacttt ggcattatca
     5221 tggtgagagg tcctgcccaa aatttttctg cgcactttac agcaaggggc ggtatcttat
     5281 ccacaagtcc ctcaagctga gaaatgtcag atatatgcac tattagagga ttgtcggcag
     5341 gtctgccctt tgcggcgaat atttttctta cagccgcttc atcaaaagcg ttcgccgcaa
     5401 gaccatatac agtttccgtc ggcataccaa cgacttcacc ctgtgagagc agctttgcgg
     5461 catatgctat gctgtcctcg tcagtgccaa gtatttttgt gtcaaacatt ttttattctt
     5521 cttcctgact tgcaagcttg gcagcctgat cggctgttgc aagtgcgtcg attatttcat
     5581 caagtgcgcc gttaagcacc tgttcaagtc tgtagatagt aagaccgatt ctgtggtcgg
     5641 agatccttcc ctcaggatag ttgtaagttc ttattctttc ggaacggtcg cctgttccaa
     5701 cctgtgaacg tctgttgtct gcgatctcct tattctgtgc ctgaactttc tgatcaagaa
     5761 gtcttgaacg gagaactttc agagcctttt ccttgttctt gaactgtgaa cgctcgtcct
     5821 gacactcaac taccatgcct gtaggcaggt gagttattct gatagcggaa gatgtcttgt
     5881 tgatgtgctg tccgcctgcg cctgatgaac ggaaaacatc tattttcaga tctttttctt
     5941 cgttaaggtc aagctccacc tcgtcagcct ctggaagcac ggcaactgta acagtagacg
     6001 tgtgaactct gccctgtgac tcagtttcag gtacacgctg aactctgtgt actccgcttt
     6061 catatttcag tcttgaataa gctccctcgc ctgaaacgct gaaagttatt tccttatagc
     6121 caccaagctc tgtttcgttg gcgttcatga cttctatggt ccagcccttt gaagcggcat
     6181 acattgaata cattctataa agagagcctg caaacagcgc agcttcttca ccgcctgctc
     6241 cgcctcttat ctctatgata acgttacgct catcgttagg gtctttcggc agcaggagaa
     6301 gcttaagctc ttcttcaagc ttagccatat cttccttgga ttcttcaagc tgcatctgca
     6361 ccatttcctt gaagtcctta tccattccgc ctgcgtcaag aagctcgatg gcttcgtcat
     6421 tggcagcctt tgcctttttg tattcaccga acttttcaac tatcggcgta agtgtcttgt
     6481 attctttcat aaggctcgta tacagtgaag tgttgtttac agtttcaggc tgcatgagct
     6541 tttcgtttat ttcattgaat ttatcttcga gtgctttgag tttttcaaac atagttatat
     6601 tctcctgtca tttttatttc atatcataca tttgttatat atcataaatt gtgagaatag
     6661 ctatgactca gcctctctga taatagtttt tacgttcttt tcggctttgc tgcgtactat
     6721 catgaactct cgtccgcagc cctcgcagcg cagcttgaaa tccatgcctg agcgcagaac
     6781 tatcattctg ttactgccgc aggggtgact tttcttcatt acaagaatat cgccttttac
     6841 aatatccatt ttgcaccact ttcctttcat aaaaggtgaa gcagagcgaa tactctgccc
     6901 atacatcgtt attataacac atttctccct ttattgcaat agcggagagg gcggttttat
     6961 tgctgtacaa atattatagt ccatatattt gatttttgtt gcattgttac aaaaatgaca
     7021 ttataagaag ctgaatttac aaaatgttca ttgaatagct tttggaaaag tgttaatatt
     7081 agattgttca aatttgaaat atctaatatt ggaggtttga caatgaacga taatatgaca
     7141 acagcaacgc atatgtttga tcattcaaag aagcttgcgg aagcatatga aaaagtcatg
     7201 cagccccttt gtaaaaagct cgatatgcca aagacggcgc ttgacgtgct catgttcctt
     7261 gcgaacaatc ctgagttcaa cacagcgggt gatgtgtgca gatacaggaa tatcaaggcg
     7321 gcacttgtgt ctttccatgt agaaaagctt gtggaagcag gctttgttga aaggcaggcg
     7381 gtaaagggcg acagaagaaa atgccgtctg gtttgcacag aaaaggccca gcctgttata
     7441 aaagagggca gggagctaca aaaaaagttt gcacaaaagc ttatcgctgg cttttcggac
     7501 gatgacatgg cacatttcag aaaatgcctg catatcgtta gcgacaatat agacagcatt
     7561 cttaaagaat gtttgtagaa ggaggtagca actatgataa aactgcttaa aaagatgggc
     7621 aagcgtgagg tgcttatggc tgttctctgt acgatccttg tgctgggaca ggtgtatttt
     7681 gacctgacac tgcctgacta tatgacagac ctgaccatga tgctcaacac ggcaggcagt
     7741 gaaacttccg atatcctgaa cgtcggactt aaaatgctgg gctgcaccct tgcaagtgcc
     7801 gctcttgcta tcggctgcgg ctatctttcc gcaaagactg cctcaggctt cagctacact
     7861 gtaagagaaa agctttttaa ccacgttatg gatatgggaa gcgaggagat gcaggacttt
     7921 tccataccta gtcttataac aaggacaaca aacgacatta cacagataca gatgataata
     7981 tcaatgggct tgcagatgat aataaaatct cctatcatgg cggtttgggc tgttataaag
     8041 atacttggca agagctggga gctttcagcg gtgactgcgg cgttcgttgt ggtgatatgc
     8101 gtattcgttc ttgcagttat ggcgacctgc ataccgagat tccgtatcgt tcagaagcta
     8161 actgataaaa taaaccgtgt ggcaagagaa aatctcacag gcataaacgt tgtccatgct
     8221 ttcaatgctg agaaatatca gaacgacaag ttcgatgttc caagcaagga tatgatgaac
     8281 acacagctca aaaaccagag aatgttcgca cttatgatgc cagttatgaa tatcggaatg
     8341 aatggtctga cccttgctat ctactggctt ggagcagtgc ttatccagca gatagccctg
     8401 acggctgttc aggacaggat aacgtttttc agcaacgtgg tggtattcag cacatatgcc
     8461 acatacgttg taatgtcatt tatgatgctc gttatgatat tcatgatgct ccctgcggct
     8521 caggtatctg cagagcgtat caacgaagtg cttgagcgtg acgtgaacat aaaagagggc
     8581 agcgtcagcg agggaagaga gcagggtact gtggaattta aaaacgtttc tttcagatat
     8641 cctcatgcct ctgaggacga gctgagcaac ataagtttca agatagaaaa gggtcagacc
     8701 cttgccataa tcggcgcaac aggaagcggc aagacaacgc ttataagcct tataccaaga
     8761 ttttatgacg caacagaggg cgaagtcctc gtggacggcg taaacgtaaa aaactacaag
     8821 ttcgataccc tttatgacaa gcttggatat gtaacacaga aggctgtgct ttttgcagga
     8881 agtataagag aaaatgtttt ctttggcgag agtgccgctc ctgaaactga cgaggagctt
     8941 aaaaacgcta tcgagctttc tcaggcggag gagtttgtga ataagcttcc tgacggcaca
     9001 gagcatatga tatctcaaat gggcaggaac gtttcaggcg gacagaaaca gcgtttgtct
     9061 attgcaagag cgctttcaag aaagcctgag atacttgtgt ttgatgattc tttctcagcc
     9121 ctcgactaca agacagacgc aaagctcaga gagggtctta acgagaagct taaggacaca
     9181 acaaagataa tcgttgcaca gagaataagc actatccgtc atgcagacaa gataatagtc
     9241 cttgaccgtg gcgaagccgt gggcatgggt actcatgagg aacttatgaa aaactgcgac
     9301 gtatacaaag agatagcact ttcacagcta agtgcggcag agcttgcata gaaaggggga
     9361 ctgcataatg agaaaatacg aaaaggctag taagggcgaa atggcgtccg ctatgaaaaa
     9421 aataatagag tatatgaaag gc
//
LOCUS       DAWEMR010000098         9426 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3036_length_9426_cov_12.269128, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000098 DAWEMR010000000
VERSION     DAWEMR010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3036_length_9426_cov_12.269128"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2761 a         1934 c         2364 g         2367 t
ORIGIN      
        1 tgcaacaatt gcagaaatgt tgccaaatta aaatataaat catttgtttg cacaatttgt
       61 taagtaagtt tttgttgaat atgacaagta agtcggaatt attatgtaat ggcagacaag
      121 ttgatgcaga tagtgatgaa ataaagggta ggttatggtg gaggtaatga ggcggagggt
      181 gataaaagcg ggcgagtaaa cgaaaaattt acgatttgat aacacattgg taatagtaga
      241 agtttctgcg tcgaattgtg cgatttttgt tcaaatcgga gaaaatagga aaatcggcga
      301 gaagtgtaag ctaaaatgct tgacaagggc ttgctaatat tatataatag tatactgtat
      361 cataatggag ttttccacct atttcatgcg tttttgagtg cataaaatca tggaaaacac
      421 cgccaaaacc actggcgaat acgattgtta tatagtatta tataaatgta ttttccaaat
      481 aaattaagga gtgattgagc ctatggtgaa tgtcaaggac gtaaaacttg gtaagaacac
      541 gagaaagagc tttgccaaga tcaatgaggt tctggagatg cctaacctta ttgaagtgca
      601 gaagaactct tatcagtggt tcctcgatga aggtctgaag gaggtcttca gagatgtttc
      661 agctattact gattataatg gtacgctgga gctgactttt gttggatacc atttcgacga
      721 agaggctaag tattctgttg cagagtgcaa ggcgagagat gtaacttatg cagtgccact
      781 gagagttact gcaagactta acaataccga gaccggcgag atcaaggagt ccgaggtatt
      841 tatgggtgac ttccctaaaa tgaccgacag cggaactttt gttatcaacg gtgcagagcg
      901 tgttatcgtt tctcagttgg tgcgttcacc aggtgtttat tacgcttttg ataaggacaa
      961 gaccggtaag gatctgttca agacaactgt tatccctaac agaggtgctt ggcttgaata
     1021 tgaaatggat tctaacgacg tggtttatgt cagaatcgat aaaaacagaa agattcctct
     1081 tactactttc ctgagatcgc tcggtattgg tactaacgag gagatcgagg aagtcttcgg
     1141 tcctgatgaa agacttactc agactatcat gcagaaggat cagactgcta acagagagga
     1201 agctcttctt gaagtttaca agaagctccg tccgggcgag cctcctacag tagattctgc
     1261 tgttactcac ctgaataacc tgttctttga cgctaagaga tatgatcttt caagatttgg
     1321 cagatataag tacaacaaga agcttggcgt tggttcaaga ctttcaggcc acagactttc
     1381 aagacctgtt gtaaatccta tgacaggcga ggtaatggct gaggctggcg atcttatcag
     1441 ctttgataag gctatggaga tcgagaccgc aggcgttatg gaggtttacg ttgacgttga
     1501 ggtaaaggag catcttactt ccgcaacagg cgaggctgtt acaaagcttg aagagtgtga
     1561 agttaagatc ataggcaacg gcatggtaga catcaacgct tatgttgatt ttgactgcac
     1621 tgagcttggc atcaacgaaa aggtatcttt caaggctctc aaggaagttc ttgaagattc
     1681 tgagaatgag gaagagctta aggagaacat cagacttaag gctgacgacc tcgttccaaa
     1741 gcacatcact atcgatgata ttatcgctac ggtttcttac ttcctcaacc tttgcgaggg
     1801 cgttggtact gttgacgata tcgaccatct tggcaacaga agaatccgtt cagtaggcga
     1861 gcttcttcag aaccagttca ggatcggttt cacaagaatg gaaagagtta tccgtgagag
     1921 aatgaacatt cagtcacagg gcactgaggt cgttactcct acagctctta tcaatataag
     1981 acctatcact gcggctatca gagagttctt cggttcttct ccactgtcac agttcatgga
     2041 tcagaacaac cctctggctg agcttactca taagagaaga ctttcagccc tcggccctgg
     2101 cggtctttca agagaccgtg ccggatttga ggtccgtgac gttcactata ctcactacgg
     2161 cagaatgtgt cctatcgaga cgcctgaagg tcctaacatc ggacttatct cttaccttgc
     2221 ttctttcgca aggataaacg agtacggctt tatcgaagct ccttacagac gtgttaacaa
     2281 ggaaacaggc gttgtaactg acgaagttgt ttatatgacg gctgacgttg aagataacta
     2341 catggttgct caggcaaacg agcctcttac tgaggataac aagtttgcca gacctaaggt
     2401 aaacggcaga tacagagatc agatcctcga gatagagaga gagaagatcg acttcatgga
     2461 cgtttcacct aaaatggtag tttctgttgc gactgcttgt atcccattcc ttgagaacga
     2521 tgacgctaac cgtgcgctca tgggttcaaa catgcagaga caggctgtgc cgctgctcaa
     2581 gactgagtct cctatcgttg gtactggtat ggagtacaag gcttgtcttg attcaggcgt
     2641 tgctgttgtt tctaagaacg caggtgttgt tgagagcgtt gacgctgaca agatcgttat
     2701 ccgtgaggac agcggtatgc tcagaactta tgagctgact aagttcaagc gttcaaacgc
     2761 aggtacttgc acaaaccaga gacctatcgt caacaagggc gagagaattg aagctaatca
     2821 gataatcggt gacggacctg ctacctcaaa cggtgagctt tcacttggta agaacgctct
     2881 tatcggcttc atgacttggg aaggttacaa ctacgaggac gccgttcttc tgaacgaaaa
     2941 ccttgtaaag caggataagt acacttctat ccatattgaa gagtatgaga cagaagccag
     3001 agatacaaag ctcggacctg aggagatcac aagagatatc cctaacgttg gtgaggacgc
     3061 tcttaaggat ctggacgaga acggtatcat aagaataggc gcagaggttc gctcaggcga
     3121 tatacttgtt ggtaaggtaa cacctaaggg tgaaactgag ctgacagcag aagaaagact
     3181 tctgagagct atcttcggtg agaaggctag agaagttaga gataattctc ttaaggtacc
     3241 tcacggcgag gccggtacta tcattgatgt taagatattc acaagagaga actgcgacga
     3301 gctttctcct ggagtaaata tgctcgtcag atgctacatc gcacagaaga gaaagatctc
     3361 tgtgggcgac aagatggcgg gtcgtcacgg taacaagggt gtcgtttcaa gaatactccc
     3421 acaggaggat atgccattcc ttgaggacgg tactccgctt gatatctgtc ttaacccatt
     3481 gggcgtacct tctcgtatga acatcggtca ggtgcttgag gttcatcttg gtatggctgc
     3541 aaaggctctt ggctggaaga taatgacccc tgtattcgac ggtgctcacg aggcagacat
     3601 cagagaatgc ttcaaaatgg ctcagcagaa ttatctggag cataaggacg atgagttcac
     3661 tctccacaca atggacgagg tcatcaaccc taacgcactg agcatgagcg aggacggcaa
     3721 gttcactgtt tatgacggaa gaacaggcga gaagttcgat aacagagtta cagtcggtta
     3781 tatgtactac ctgaagctcc accacctcgt agacgataag atacacgcac gttcagttgg
     3841 tccttattca ctggtaacac agcagccact cggcggtaag gctcagttcg gtggtcagag
     3901 attcggagaa atggaagttt gggcgctcga agcttacggt gctgcttaca cacttcagga
     3961 gatccttaca gttaagtctg acgatactgt gggaagagtc aagacatacg aggctatcgt
     4021 taagggacag aatgttccta agcctggtgt tcctgaggcg ttcaaggtac ttgttaagga
     4081 gcttcagtca cttggtctgg atctcaaggt actcaacatc aataacgagg aaatagatct
     4141 taaacagtcg tttgacgagg acgaggagct tgttccacag cctgctatcg aagatcatat
     4201 cgatttcggc gttgacgagg gcgatctcga cgagggcttc atggctgaag acgagaacgg
     4261 cacttccgaa ctggagggca gcgcagcttc cgacgagtct gatctgttcc ttaatgacgg
     4321 tgaataattt tgcacagtaa ctaaataagc tgaggtcaaa gggcggaagg ctccgccctt
     4381 gcgaccgttt catatagaag tcggctgttg ccgtgcttca aactaaacag gaagagggta
     4441 ctgctgttgg aattcaatga gtttgcatca atcaagatcg gtcttgcttc gcctcagaag
     4501 atccgtgagt ggtcttacgg cgttgttacc agacctgaaa caataaacta tcgtactcag
     4561 aagccggaaa aggacggatt gttctgcgaa aagatattcg gaccttcaaa ggactgggag
     4621 tgtcactgcg gtaaatataa gaaaattcgc tttaaaggca aagtctgcga gcgctgcggc
     4681 gttgaggtaa caagagccaa ggttcgtaga gagagaatgg gtcacatcga gcttgctgca
     4741 cctgtttctc acatttggta tttcaaggga actccgtcaa gaataggaca gatgcttgaa
     4801 atatcacaga aaagacttga agaagttctt tacttcacaa aatacatcgt tatagatcca
     4861 ggtaacactg agcttatcaa gggtcagctc cttactgaaa aagattatag cgaaatgcag
     4921 gaaaagtatg acgaggacga gttcagagcc ggcatgggcg ctgaggctat caaggagctt
     4981 cttgaagaga tcgacctcga gaagctctca gaagagctta aaactgagct gaaggatctt
     5041 cctgcgggtc agaagcgtat caagcttctg aaaagactgg agatcgttga ggctttcaga
     5101 acatcaggca acagacctga gtggatgata ctggaggcta tacctgttat accacctgat
     5161 atcagaccta tggtactgct ggacggcgga agatacgcaa ccagcgacct taatgacctt
     5221 tacagacgtg ttatcaacag aaataaccgt cttaagaaaa tgctggagct tgaagcacct
     5281 gacatcatcg ttagaaacga aaagagaatg cttcaggaag ctgttgacgc acttatcgac
     5341 aacggcagac acggcagacc tgttacaggt cctaacaaca gagcatacaa gtcactttca
     5401 gatatgctca aaggtaagca gggtcgtttc agacagaacc ttctcggtaa gcgtgttgac
     5461 tattcaggac gttctgttat cgtcgtaggc cctgagctta agatgtatca gtgcggtctg
     5521 ccaaaggaaa tggctcttga gctgttcaag cctttcgtta tgaaaagact tgttgacaca
     5581 aatcctacta tcaacatcaa gtctgcaagg aaaaaggtag acagagctga gccagaggtt
     5641 tgggacgcac ttgaaaacgt tattcagggt caccctgtaa tgctcaaccg tgcgcctaca
     5701 cttcacagac ttggtattca ggcattcgag cctatccttg tagagggcag agctatcaag
     5761 cttcacccac tggtttgtac agcgttcaac gctgacttcg acggcgacca gatggctgtt
     5821 cacctgccaa tctcagcaga ggctcaggct gaggcaagat tccttatgct ggctgccaac
     5881 aacctgttga agccttcaga cggacgccct gttgcagttc ctacacagga tatgatactt
     5941 ggttcttact acctgacact gaagaaggac ggcgagcctg gcgagggcaa ggtgttcaga
     6001 gatgtgaacg aggctcttat ggcttacgat cagcacatcg tttcacttca tgctgctatc
     6061 aaggtaagaa ttaccaagca gataggcgac aagcaggtat caaaggtgat agacgctaca
     6121 gtcggaagac ttatcttcaa ctctatcatt cctcagaacc ttggctttgt tgacagaaca
     6181 aattcagaaa agcagttcga tcttgagata agcttccttg taaagaagaa gcagctttct
     6241 gatatcattg ataaatgtat ccagaagcac ggtactacta cgacttccga agttctcgac
     6301 aacatcaaga gccttggtta taagtattct acaaagtctg ctatcacagt tgctgtttgc
     6361 gacgcttcta tccctgaggc taagaaggat atccttgcaa aggctgacgg acttgttgac
     6421 aagatagaca agcagtacaa gagaggttat atttcaagag aagagaagtc aaagaagttc
     6481 atcgagatct ggaacaacgc tacagacgaa gttacagagg ctctgaaagc taaccttgac
     6541 ccatataacc caatcaacat gatggctgac tcaggtgccc gtggttctat caaccagatc
     6601 agacagctgg caggtatgcg tggacttatc gccaacacat caggttcaac tatcgagatg
     6661 cctatcagag ctaactaccg tgaaggtctt aacattctgg aatacttcat ttcttccaga
     6721 ggtgcgagaa agggacttgc cgatacagct ctgcgtactg ctgactcagg ttaccttaca
     6781 agacgtcttg ttgacgtttc tcaggacgtt atcattcgtc aggaggactg cggtactcac
     6841 gttggtatcg agatattcga cataaagaac ggaaacgaaa tgatcgagcc tctcagcgag
     6901 agacttgttg gcagatacct tgttgaggac ctcaaggatc ctaagacagg cgagatgata
     6961 tgctcaagaa acaaactcat aaacgttcat gacgctgaaa gaatagttgc tgcactcgaa
     7021 gctgagggca aggattctat ctctatcaga tcagttcttc agtgtcaggc taagcatggc
     7081 gtttgtgcta agtgctacgg cgctaacctt gcaaacggaa atatcgtaca ggtcggtgag
     7141 gctgtcggcg taatctccgc tcagtctatc ggtgagcctg gtacacagct tacaatgaga
     7201 accttccaca caggtggtat cgcttctgcg gaagatatca cacagggtct tcctagagtc
     7261 gaggaactgt ttgagagcag acgtccaaag ggtgctgcta tcatcacaag gatcgacggc
     7321 gtagttagga tcgaggaggt caagaagacc aagcagatca ctgttacaag cgaggacact
     7381 gaaaatcctg agcaggagtc ttatgctatc ccatacggct acaagataag agttcgtgag
     7441 ggacaggctg ttacagccgg tgagccactc acagagggtt ctatcaaccc agctgatatc
     7501 cttgcagtta atggtcctaa ggctgttcag aactacatca tcaccgaagt tcagaaggtt
     7561 taccgtctgc agggtgttga tatcaacgat aagcacatcg aagttatcgt tcgtcagatg
     7621 atgagaaagg tcaagatcga cgactccggt tcaagctatc tgctccctgg ttcacttatc
     7681 gacagaggag aggtcgcaag actcaacgag gagatacagg ctcagatcga cgctggcgac
     7741 gagaacgcaa gacttatcac aactgttcct gttcttcagg gtatcacaaa ggcttcaagc
     7801 tactcggatt cattcctgtc ggctgcttca ttccaggaga caactaaggc tcttacagac
     7861 gcagctatca gaggcaagac cgataagctc atgggtctta aggaaaatgt tattatcggt
     7921 aagctcattc ctgccggcac cggtatggac gtttacaaca acgttcaggt ggtcaagaat
     7981 gagagtgcgg ctaaccataa caccgcagaa acaccgttgt actaatgact tttatatcag
     8041 cctgcatggt tcattcatgc gggctgattt ttttattttg tatgaaacct ttttgtctgc
     8101 cacggcgttt tagtatatga agggaagtga aagcatgaag aatatgaccg aaagcgaata
     8161 ccgccaactt gtagaggagt attcggatat ggtaacaagg ctttgcgtgg tgcacacggg
     8221 aaatttttcc gacgctgagg actgctatca gaacgtgttc ttcaagcttt tcaagcagat
     8281 gaaaaaaggc agtctgccaa acccgaaagc gtggctcatc agagtggctc tcaacgaatg
     8341 ccggagtgtt ttgcggtatc gtttgaggaa aaacacagtc gatctgaaca gcataacgtc
     8401 tttctcaaag gacggcagcg acatagagat acttgacctt attttcagac ttcccccgaa
     8461 atatcgggat agtatttatc tttattactt tgaggagctg tcggtgcagg agatagctga
     8521 gcttactaag ataaaggaaa ataccataaa aacgcatttg aaacgtggaa gagaacaact
     8581 gaaggatatg ataatgttgt gaactatatg aatgtcaata aagacataaa gtatcgggac
     8641 gtcgagggct ccgtccccta caaaagcggt aaaaaacatt aagtaggggc tggcatttta
     8701 gacataacat tatagtttgc aatgataata tcacagaaag gagcagaaaa tgaaaaaaat
     8761 aaacgctttt tactgcatta aaactccttc ggattggaag gagagtgtgc ttatgtatga
     8821 caagcctaca gagaaaaata aaacaaaact tacaggcttt cagcttgcag gtgcggtgct
     8881 tgcggctttg cttctggtgg tgggcggtgc gctgaccctt tatcgcagcg gtgatgagca
     8941 taagatcaat gtcggtatgc agcagccaag tatagacagt tcggaaagcc ttgacagcac
     9001 tgagcttacg gctgatttag aacaggcaca aaatgcgctt aagcaggtcg ttaaagacaa
     9061 agagataacg gcggtcggaa cgtatgccgg ctgcggctcg gacggaacgg gatatctttt
     9121 tgtcgataag atatatgttg agaaatacac ctctgaaaag ggcggattga tcgactatgt
     9181 tactgtgaat gaatatgaat acctgaaata ccactattac gctgcgttta aacttgattt
     9241 cagagcgaaa gatgttacca cagggaagtt tgtttatgcg gacgaactga aagtgggcga
     9301 aaaaatcgca gtcggcacaa actgcgataa tctgagtaat tggaactcgg atgagatcgc
     9361 aacaggtctt ggcgaaacgg tcggtggcga cagctttgga acagacttct attattcact
     9421 tcgatc
//
LOCUS       DAWEMR010000099         9419 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3041_length_9419_cov_10.102520, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000099 DAWEMR010000000
VERSION     DAWEMR010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3041_length_9419_cov_10.102520"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2720..2729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2713 a         2259 c         1708 g         2729 t
ORIGIN      
        1 aacgctttca catatttccg ccacaactcc taggttatga gtgataaata ttaccgaggt
       61 gttattttct gccgcaagtt ccttgataag tttgagtatc tgtgcttgta tcgtaacatc
      121 aagagctgtt gtcggctcat ccgctataag aagtctaggt ttacaggaga gcgccattgc
      181 tatcattact ctttggagca ttcctccact catttcaaat ggatactgtt cataccgctg
      241 ttcagcagga agtattccta ctttttcaaa caaagtaagc acatattcct ttgcctccgc
      301 ctttttcatt ttcaactttt tgcgcacaag ctcagcgatc tgctcgcctg ctttcattat
      361 tgggtcaaga gatgtcatag ggtcctgaaa tatcattgca atatcttttc cctgaatagt
      421 ctgcatctgc ttctccgaca gcgaaagcac atcaacttct ttgccctcgc cgttcttgaa
      481 aaggatctgg cccgaatatc tcgtatgctt ttcatcatgg agccgcataa tagatttagt
      541 ggaaacgctc tttccgcagc cgctttcacc cacaacgcca tggatctcgc cctcctttac
      601 gctaaggtct actccctgaa cagcgtaaac tatacctctg accgtcataa aatctacaga
      661 cagatctttt atttcaatta cattatgctc gttcataaga acacctactt actttttata
      721 ttgctgcctt ttgacggcgc tggatatgga taagtctctt ttcatactgt ttgaatgtcg
      781 gaagctttct ctgtgacgga tcgatatact cttgaataca gtcgctgaaa atattaaccg
      841 ctacggtcac aagcattata attattgcag gggcagcaag aagcacataa ttgttataga
      901 aattctgcat accattttca agaagagttc cccaatctgc gtcaggtggc tgaatgccaa
      961 gacctaaata gctgagcgtt gccatcgcta ctatagccgc accgatatcc attgtgaact
     1021 gagcgatcat tgtggtgata acatttggaa gaatgtgaaa aaagattatc ttcatatccg
     1081 aatatcccat tgacttgcag gcttcaacat aaaccgcttt cttttccgtt atgataagcg
     1141 aacgcgccat ttttgctgtc agcggtataa atgcgatacc aatggacata acgcctgtga
     1201 aatttcctct gccgaaaatt gcgataagta tgaatgaaag aagcaaagac gggattgaac
     1261 agacaacatc ccagattctc attataactg cgtcgatctt tcctccgtaa tatccgcata
     1321 taagaccgat aggtacgccg atgaccacct caaaggcaac tatgaggaaa gcattgagca
     1381 tagagctttt tgcacccatt ataagccttg ataaaaggtc acgtccaagc ttgtccgtgc
     1441 ctaaaatatg tccttttgag aaagcaggaa gaaaacagga tcgtggatca acttcatttg
     1501 gatcataagg aacaatgaca tttgcaccaa tagcagcagc tattgctata agtatcacta
     1561 tgcctgaaat aaccgttttt ggagtaaaaa actttcccac ctttgaacgt ttagccatta
     1621 tccattcctc cctacttcat ctcaacacga gggtcaacca ctgcatagag aatatctaca
     1681 acaaaactca ttataagata tgaaacaagc attacaagca caagcacctg tactatcgga
     1741 tagttgtttt gctggatacc tgttaccagc ataccaccga taccaggaag cgaaaatatc
     1801 tgttcaataa gaacagttga acctatagct cccgcaaaca tatatcctgc tactgtaagg
     1861 acaggtatca cagcgttatg aaaagcatgt ttgaatgttt tattgaactt gccgagaccc
     1921 tttgcctttg cggttatcat atatggtgct tgaagttggg atatcatgct gcttcgtgta
     1981 actcttccca gtgttgcaac aagtccaacc gccattatga tacccggaac agatattctt
     2041 gatataaact ctgctgtatt agaatatgtt cctatgaaac tgtatccagg tacatatttt
     2101 gaaagaaaca gaatagcaag aatgcttagc acaaacgtcg gtatactcga gaccaccagc
     2161 ataagagtcg acaacgctga atctatccat gttcctcgct tcatcgccgc cagaacacct
     2221 atcagaatag ctatcactgt tcctaatatc gttgagaaaa gcacaagacc tattgttatc
     2281 ggtatcctgt ctattatctg gttgagtatc ggctgctttt gaacgtaatc aattccaaga
     2341 tcaccatgtg caatgccttt gagccaaata atatactgct tcagcagcgg ctcatcaagg
     2401 tcgaactgct ctgtatactg ttgctgaagt tcaggtgttg actgcttgtt acctatgata
     2461 acagtcagcg gagatgttgg attaagtctt aacgaaaaaa acaccaacaa cgaaaccaga
     2521 aaagttataa caactatctg tatcaatttt ttcagaatgt attttacata agccataata
     2581 acaccctttt ctataaaatg aatttttgga ttttaaatcc ttcaaattta tcttgattat
     2641 attatatact ttacagctac tgtaatgtca atatgctagc tgcaattttg tttaaaatag
     2701 caaaattcgc tgtgatacan nnnnnnnnnt attatcgttg attttgtata ttatttcatc
     2761 ttaaaaatat ggtattcgtg gcaagcaata catacgcaaa agaatgcgac catttctaaa
     2821 atgcaatttt gttaatatga tattgcaaat agcaataata agaaaggacg tctgatttat
     2881 caaacgtcct tgtatgaccc tattttcatc atcgatattc tttcaggcat ctttgcccaa
     2941 aagaatatat gacagctttt atcagttgtt ttcaacgctg cttacagctt ccgtaacatt
     3001 gtgataccat ttgcgtgatc tcagtcgtct ttgaccgaga ggaagcttaa acagctctat
     3061 agctatgtga gcagcataaa cataccaaat atcgacctta aagataagcc cgagaattag
     3121 cgtaagcggc attcctatca tccatatgct gcctgcgtcc atgagcgtaa gataccttac
     3181 atctccgccg cttctaaaaa tgccctctac cttaatacag ttcaccgcat taggtatgca
     3241 gtatatagca agtacgcaca tacagtttct cacacattcg ccctcaaggg taccctttat
     3301 tccataaata tgctgaacag gcaacataag agcaacaatg atactgcatg atacaagacc
     3361 agctatgata gaaatcctat tgaatcgatc agcatagcga taaacttttg gcatatcggc
     3421 agaacccatc tcaatgccca gcataatagt acatgccccc gcaaagccca ttattgcaag
     3481 cttcgcaaac atctccatgg tgttcagtat actcatttct gcatatactt ccgtgcctct
     3541 tgttccatat accatatagt aaaacgaaag acccagagac caaagcatat cattcgcaca
     3601 caacggaagc gaagttttaa gatactcctt gtataatcca acatttaatg ttttcaatgc
     3661 cttaaaaagg tttttcctta tcggattatc tgaaagaaag aaatatagca aaagtcctat
     3721 caactcaaca attcttgcag caagcgttcc aagcgcagct cctgtcactc caagcttcgg
     3781 cgaaccaagc tttccaaaga taaacatata gttaaaaaac atattgaaca tcatacttcc
     3841 tatgccctgt atcatcggta tcttgacatt gcctatattt ctaaaacaaa agctcacagc
     3901 atagcatatg gcgtcaagca aatatgatac cgccacgata cgcatatatg gctttgcctt
     3961 ctctataaca agaggatctc tgttaaaaag ccttattatg atatcaggaa atatcattgc
     4021 cgccaaacac atcacaagac caacagtaaa cgtgaccgtt acggctgtcc aaattatgcg
     4081 ctgaaaatct ttgatatttt tttgacccca ataacgtgtc agatatattc ctgcgccagt
     4141 attacagcca tataggaaag taaaataaac gctgaaaaaa tttgtagata ctcctaccgc
     4201 cgcaatagct gtgctaccta aggtgctgac catccactga tctatgaaag tgagacattg
     4261 gctgaatatt gaagaaacaa gcagcggcat tatgatactg agaaatttcc tgttaaactc
     4321 cttcttctca gctttaaggt caacgctttc cgacagcata tgaatcaccc ctctcatcat
     4381 ttacaaagcc gttacagttc cctttcagat gctatgttgt atgccgcaac tgcatatagc
     4441 ttgcccgcac attccgcaag attattgagc gaagtctctc cagggtcatt ataatcaagc
     4501 ataccgcata ctgaaatgcc ctttatgtca agaccaacaa gccccgtttc cattatctca
     4561 acgacctcat gagaagcaaa tcctccagca taaggtcttg ccgcagatgt cacaaaagca
     4621 ggatcataaa agttcatatc aaagatcaca aaaacaggtt ttcctgatac attctttttt
     4681 attgcagaac ccacgtctga aaacttcata taatccatgt cagaagctgt cagcacatct
     4741 atacccagct tttctagcct gccaccgtat tgtgacattc cgtttcttat tccaacttca
     4801 agagaagaag cattgtcgca catatcctct tctgccactc taacaagcgc agaacttacc
     4861 gaaccgccga agtggataac agccattttg ccatactttt catagcaagc ttttatctgc
     4921 ccataagatg tcttatcatc tccgccgatc ataaagctgc aagctcccgt ggaagctatc
     4981 gctttttcag ccgtatatat acattcctca gcggctgcct ttgaagaatc gtcgatgata
     5041 aggtcaccgc aatctccgct ctttacgcta tgcgccatat caatgtcaag tccctgatcg
     5101 caaaaaccga agccaaaaat atcacgtctg aaatatgcag aaccatttct tgaaccaggc
     5161 tttgcagttt catcacactc atatggtaat gctgcaaaaa gaatatcata accatttata
     5221 tcatagacca taggcgtttc caaaaaacct gttggcccca gatctttcag atcatcaaag
     5281 tttagtttaa atggacattt taccatatct aacaccgacc tttcctatta ttcctgctcc
     5341 gcttttttct ttgaaagcaa aataacaaac tgctttagta tcgccactgc atttatcgca
     5401 gttatgcctg cgggatcgta attttccatt acctccacca gatcaaaacc tacgatatcc
     5461 agccccgcaa gaccatttct gattatctca caggcttcat atgtagaaaa acctcctggc
     5521 attggagttc ctgtaccagg tgcataaaca gggtcaagaa agtcaatatc gaaagtgatg
     5581 aaaaccttag cgtctcccac ctgttccttt attctgcggc aagtttcctt tattccgatc
     5641 ttatgaagct ctctcgccgt gatgaccttc attccgtgat cttgagcata cttgatgcga
     5701 taagtatctc cgccgcttct tataccgacc tgaatagaat gttcagtatc aagaatatca
     5761 tcctctatag catttctaaa aggtgtgcca tgattatatt ttatcctgtc gccataatcc
     5821 caagtatctt cgtgggtatc gaaatgcacc attgcgacct tgccatactt ttcgctgtaa
     5881 gctttaagtt cagcatatgc aataaggtga tcgccgccca gaacgatagg cactgcacct
     5941 gcgtcgagta cctttctgac cgatttcctg ataagctcca ttgtctcctc aacataacca
     6001 agcacaacgc cgatatcgcc catatccatg ccttttaagt tagcgacctt atatgagtta
     6061 tcctgttcat atgacaatct gcctgcacca taagcatttc ttatcgctct agggccaaaa
     6121 cgacagcctg tcctgtttgt cacgctggta tcatagggca cgcccaaaac aacaaaatct
     6181 tcaccatcga tatcctgctt atatggaagc ccgaaaagcg tcttgatatt attttctctg
     6241 cgctttgcaa tgttgtttcg tgtctcttct gaaataattt tatcaaacat tttcgatcat
     6301 tcctttctta gcatattctt tgatgcccgc ctttttgcaa gccacacagg taataaattc
     6361 ttttacgata cggctggcaa gaattgaagt tattccccct gcgtcatagt tcggtgcgac
     6421 ttcaacaagg tcaaagccaa caatatcaag accgataagc gcacgacgaa taagctcaag
     6481 agcttgtctt gaagtaaatc cacaggaaac gggagtgcct gtaccggggg caaaagcagg
     6541 atcaacaaaa tctatatcaa atgtgaccat gaccttattg tttccgacct tttcacggat
     6601 aagctccgcc gttttctcaa tgcccatatg atgtatatca acagcataga tatgctcaag
     6661 gcctgcatta ttcgcaaaat caaaatcact ctcttcaaaa agtccgcctc tcatacctac
     6721 ctgtatagat gttttcgggt caatgcagtc atattcgatc gctcttctga aaggggtaga
     6781 gtggtcgtga tgttctttat cagtatcgct gccccatgta tctgtatgcg aatcaaagtg
     6841 tattatcgaa acaggcccgt aaacatcctt atacgccagc agttcaggaa aagtaataga
     6901 atgatcaccg cccagaacga ttggaattgt acctgtttcc aaaatagctt tcatttcctg
     6961 tggtatcgaa ccaagataag ctttctcagc gttccatata tcaagatttc cataatcaac
     7021 gccctttaca tatttactcg tatcaatgcc gacagcctcg ttcatgctgt ctgtggaaaa
     7081 ggctctgcga atcccatcag gaccaaacct cgttcccgga cgatatgttg ttcggatatc
     7141 aaatggaata cctataatag tgtaatccgc cccagatgtg tcagttgaac ttttcaaacc
     7201 gccatatgta agctgcacct ttttatcagt atttattgcg ttcatagaat cactttcctt
     7261 tcttaatttg ttgatttcat tttacgctat acagttacag taaagtcaat gctcatttgt
     7321 aaagaaaaaa ttaattctat ggcaacagat tatcctaaag cgttctttat tttctgcaaa
     7381 ctaaacgtct aaagcacata tcatattatt aatggcaaaa aaacaccccg tatcataaga
     7441 cacggggtga aatttttgtt atctattcag atatcctaaa ctttgcaaga attacttctt
     7501 ctgtgcaata gcagcctgag cagcagcaag tctagcgatt ggaactctga atggtgagca
     7561 agatacatag tcaagaccga tcttgttgca gaactcaaca gatgttgggt cgccgccgtg
     7621 ctcaccgcag ataccgcagt ggagctttgg attaactggc ttaccaagtt caagagccat
     7681 ttccataagc ttgcctacgc cgttctggtc gagcttagca aatggatcgt tctcgtagat
     7741 ctttgcatca tagtaagcat tgaggaactt accagcatca tctcttgaga aaccgaatgt
     7801 catctgtgta aggtcgtttg taccgaagca gaagaagtca gcttccttag cgatctcgtc
     7861 agctgtaaga gcagctcttg ggatctcgat cattgtacca acctcgtact caaggtcaga
     7921 gccagcagcc ttgatctcag cgtcagcagt ctcaacaacg aacttcttaa catacttaag
     7981 ctccttgata tcatttacca gtgggatcat gatctcaggc ttaactgtcc agtctgggtg
     8041 attcttctta acgttgattg cagccttgat aacagccttt gtctgcatct tagcgatctc
     8101 aggatatgtt actgcaagac ggcagccacg gtgacccatc attgggttga actcgtggag
     8161 tgaagcgatg atagccttga tagtctcaac aggcttgttc tgtgccttag caagagcctc
     8221 gatatcagcc tcttctgttg gtacgaactc gtgaagtggt gggtcaagga atctgatagt
     8281 aacagggtta ccctcaagtg cctcataaag agcctcgaag tcagcctgct gcattggctc
     8341 gatcttagca agagcagctt ctctctcttc aactgtgtct gagcagatca tctctctgaa
     8401 tgcagcgatc ctctcaggat caaagaacat atgctctgta cggcaaagac cgataccctc
     8461 agcacccagc tctcttgcct tcttagcgtc agcaggagta tctgcgtttg ttctaacctt
     8521 cattgttctg aacttgtcag cccagcccat gatcctgccg aactcgccag cgattgtagc
     8581 gtcaacagtt ggaatgatac catcatagat gttacctgtt gaaccgtcga ttgaaatata
     8641 gtcaccctcg tggaactcct tgcctgcgag tgtgaacttc ttgttagcct cgtccataac
     8701 gatatctgag cagccagata cgcagcatgt acccatacca cgagcaacaa cggcagcgtg
     8761 tgatgtcata ccgcctctaa ctgtaaggat accctgtgaa gccttcatac ctgtgatgtc
     8821 ctcaggtgat gtctcaagtc ttacgagaac gaccttctcg cctctctcgt tccaagcctc
     8881 agcatcatca gctgtgaata cgatcttacc acaagcagct cctggagaag cgccaagacc
     8941 cttgcccata ggagtagcag ccttgagtgc ctttacatcg aactgtgggt gaagaagtgt
     9001 atcgaggttt cttgggtcga tcattgcaac agcctcttcc tcagttctca tgccctcatc
     9061 aacgaggtca caagcgatct taagagcagc ctgtgcagtt ctcttaccat tacgtgtctg
     9121 aagcatatac agtttaccat gctctacagt aaactccata tcctgcatat ctctgtagtg
     9181 tttttcaagt gtttcgcaaa cctgtttgaa ctgtacgaat gcttccggga atttctgttc
     9241 catttcggag atgtgcatag gtgtacgaac accggcaaca acgtcttcac cctgagcatt
     9301 tgtaaggaat tcaccaaaca gaccgttagc tcctgtagca gggtcacgtg tgaaggcaac
     9361 acctgtacca cagtcatcac ccatgttacc gaatgccatc atctgtacgt taacagctg
//
LOCUS       DAWEMR010000100         9230 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3119_length_9230_cov_7.764251, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000100 DAWEMR010000000
VERSION     DAWEMR010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9230
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3119_length_9230_cov_7.764251"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5470..5479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2480 a         2237 c         1699 g         2804 t
ORIGIN      
        1 gatgatagct gtaacaaact ttttcattaa aaatggcctc ccataaatat ctgttgttca
       61 gattatggga ggtcatagtt attttattca gtttccgaaa aattttcttg cgatatctac
      121 cgactctttt gcgtcttttg cataatagtc agcgcctatc tgctctgcat agtcagccgt
      181 taccaccgca ccgcctacta ttatcttgca gtcaacattg ttttcacgaa gaagcttaac
      241 cgtattttcc atgctcacaa gagttgttgt cataagcgca gaaaggccga taagatgtac
      301 gtcattttct attgcacaat caactactgt ctgatagtca acgtccttgc caaggtcaat
      361 aacgtcataa ccatagtttt caagaagtac ctttacaatg ttcttgccga tatcgtggat
      421 atcgcccttt actgttgcaa ggattatctt gcccttagaa acgctttttt cacccgttga
      481 cagaatgtaa ttttttatta cctcaaagca agcctgtgca gtgcctgctg tctggataag
      541 ctgcggcagg aaaagcttgc ccttttcaaa ttctgcacca gccttatcaa gtgccgggat
      601 aagcatatcg ttgacaatgt tcatggcttc tgtttcctgc aaaagctttg cagtaaggtc
      661 agctgcttct ttcttcagac cgttttcgat agcatacggc aagtctatct cagcttttgc
      721 gcctgttgcg gcaggagcag tttttttctc gccaccgtag tgagaaataa agtcaacaga
      781 gtttttatca atgtttgtca gaagcttgta ggcacgaaca gttgccgtca tagaatcaat
      841 attaggattc attatcggca ggtcaagacc ctttgtgagc gccatcataa gaaagttgtg
      901 attaactatc tctctgctag gcagaccaaa gctgatattc gatacaccga gaactgtttt
      961 aagtccaagc tcgttcttta ctcgctccac tgcatttact gtctgcataa cattttcctg
     1021 ctccgcagaa gcggtaagag taagacaatc tatatacaca tcttcttttc taatgccgta
     1081 ttcaagcgcc ttgtcaagta tctttttagc aagctcaaat ctcttgtcag cactctttgg
     1141 tatgccgttt tcatcgagag taagacctac cacagccgca ccatatttct ttacaagagg
     1201 aagaacattt tcaatggagc tgtcctcagc gtttacagaa ttgacgatag gcttgccatt
     1261 ataagccctc agagccgctt caagcacctc aggtatagtt gaatccacct gcaaaggcac
     1321 atcgacaacg ccctgcaaag actttacagc cttgaccatc atactctttt cgtcaattcc
     1381 cggcagacca acatttacat caagaatatc cgcacctgcg tgtatctgct cgatagcctg
     1441 accaagaata tagtcgatat cgtttgcaag cagtgcttct ttaaaacgct tcttgccagt
     1501 aggattgata cgctcgccga ttattcttgg ctggtctata actacagtat gtgtcggtga
     1561 acagcaagcg gcagggatat cattatgcac gctgacgtgc ttcttgcctt tgagcatttc
     1621 agccagcttt ttgatatact cagggttagt tccacagcaa cctccaagca ccttaactcc
     1681 caatgggata agcttttctg caaactctgc aaaatcttca ggggaacagt tatattcatt
     1741 tgtaacaggg tcaggaagtc ctgcattagg ctttactaca acagggagat ttgtccactt
     1801 gcaaagctcc tccacaacag gataaagctc ctttggccct aatgagcagt tcacaccgat
     1861 tgcgtcaacg cccagaccct ccaaagtcaa agccatggag cttatggcaa cgcccgtaaa
     1921 tgtacgcata ttttcctcaa aagtcattgt gcagactata ggaagatcgc tgtgttcctt
     1981 tgccgcaaga acagccgctt taagttcata gagatctgtc attgtttcaa aaacgataag
     2041 gtctgcgtca gctcctgcaa gtatctgctc cttataaata tcgtaagctt cttcaaaagt
     2101 gagcgttcct gttggctcta gaagctgtcc gataggtccg atatcaagag caacaagggt
     2161 atcagtaccc tcacaggctt ttcttgcatt ttttatagcc gcaggaatag cctgctggac
     2221 agtatatccg cacttttcaa gcttgtgtct gtttgcaccg aaagtattgg cgtaaacaat
     2281 gtctgagcct gcgtttatat actggcggtg tatgtccacc agccaatcag gcttgtctat
     2341 attaagagct tcaggagtgc cacccatttc aagtcccttt gcctgaagca tcgtacccat
     2401 tgctccgtca agtatgacaa attcttttgt tttaagtaat gttctaaaat ccacaatgat
     2461 ctcctctttt tctgaattga cagacttccc tcatattaca gcaggcacaa ccacgtctgc
     2521 cctttgatat ttcatttttt gacaatccga ttatcgccgt aacagacttt ctcggcgtca
     2581 gtatattatt ttcagttgca ttaagcccga taaatttctg cgcattgagt acgtcaagaa
     2641 aaactctctg cacgctgata ggcagatcgc cataaccagg agaaaacctc catgtctgat
     2701 tatatccgct gagcttttct ttcacagtgt tgtcaactat atcgcatacc tgctccacag
     2761 ccgcacttgc aaggcaatcc gctatcatag ctttagccat atcctctgct tcataagcac
     2821 gaagccttct atcaacgccc gaagaaatag tggctgcaaa aagcacgcat ttactgcacc
     2881 cttcaaggtg tctgcatata tcattgccgc caagaaacaa cggagtaccg atgacctcaa
     2941 caccgttctc tttatgagcg acatcaaaca ctctgtacac aaatttaggc tcaatgacct
     3001 tcaaaagctc tttttcacat tcatctatca acaaagccgt gcgttcgtct ggcttgttat
     3061 tgcccattcc catatatctc acagcttctt tgacgtttat atgttcaagt ttcaaataat
     3121 cacctaccga ttgttattat accacattac tatgaatttt acaacaataa ataataaatg
     3181 tacgttcaca aaaaagtcca cgcaatcatc agggactttg tgttacatat tgtcagcgtc
     3241 tttaagatac tgttcagcat tttcaagcat aagctctgtg atattttcac cgcccattat
     3301 ccttgctatc tcatactttc tctgctcatg atcgagagtt ctaacagttg taactgtcct
     3361 gtcgccctgt atatttttct caataagcaa atgattatcc gccataacag ccatttgggc
     3421 aaggtgggtc acgcaaagaa cttgcctaag gcgagatatc tgtttcagct ttatacctat
     3481 cttctgagcc gctctgccgc ttactcctgt gtctatctcg tcaaatatca gcgtgccgat
     3541 actgtccttt tctgcaatta cgtttttcag tgcaagcatt attcttgaaa gctcaccgcc
     3601 tgaagcgatc ttcgcaatag gctttggctc ttcgcccagg tttgcagaaa taaggaactc
     3661 tatgctatcc atgccattta tggtgagctt gcctgttttc tgctgaacca ccagcttgac
     3721 ctttggcata ttgagaaatt caagctcctc tgttacctga gaaacaaacc gttctcctgc
     3781 tttttttcta tggtctgaaa gtgctttggc tttccttgat acctccgcaa gaagtctttc
     3841 cttttcttta ttaagctcgt ctaagctctg ttctgctccg ctcagttcat caagctcatt
     3901 ctgtgaattt tcaagagtag tcagtacatc atcaagttca ggtccgtatt tcttcatgat
     3961 acgcctgagt tcatcagaac gctgattaag gtaatcataa cgtttcgggt cgatatcgag
     4021 gctgtcaaga agcgagccta tctcctcgga aatatcctcc atttcaatag ccgcagaact
     4081 aagtctgtca tatataggcg atatctcagt cattatatcg gtatattcct caaccgattt
     4141 tgaagctctc tgcgtcatct ccactgcacc atctgtgtca tcatcgcccg aaagaagttg
     4201 ttttgccata taaagagcct cagagattgc aacagcattt ttagagatgt taagctctgc
     4261 ttctatctct ttatcctcgc cttcatgaat gtttagcgcg tttatctcct cgacaatatc
     4321 agcaagctca gccatgcgaa actctttttt gctttgctca gtttttatct tgtttatctt
     4381 ctttgcaatg ctttgaagct ctctgaaaga gctgtgataa tcttctataa gagcttccga
     4441 ttcggcataa ctgtcaagta tctctatatg acgttcagga gccatgagta tctgattgtc
     4501 gtgctgtcca tggatagtta caagcgtttc tccaatagct ttaagtacgg agacgttcac
     4561 cgctctcgag ttaactcttg caacgcttcc accatctgaa cgtatctcac gggtaagaaa
     4621 aagctgtccg tcttctgcac ttatgccaag ttcgtctagt acctgcaaaa cattgtcccc
     4681 aatgtcagta aacaacgctg aaataacagc cttgtccgtg cctgttctca caatatcctt
     4741 agtgacacgc tgaccgagaa tagcatttat tccatttata agtatggact tacctgctcc
     4801 cgtttcgcct gtgaaaacat taagtttatc agaaaaatct atagtcgcct tttctatcac
     4861 ggcaagattt tcaatataaa gttcacttaa cattttagat cattcctctg ctgtttaaga
     4921 aataatagag gatctcagtt cctctgccat atgggacgca tctttttctg tccgcataag
     4981 tatgaatatc gtgtcgtcac ctgcaagcgt tcccacaaca ttgtcaagcc caagcttgtc
     5041 aagctttgca cacaccgcct gtgccatgcc cgtatgacat ttcacaacta cagtgttaag
     5101 cgcatgatcc actgacacaa cggcgtctga aattatgccg aaaatgccgt tgttttgctc
     5161 tgcaatatct tttttgagtg caggcaggct gtatttataa ttgccgtctg aaccaatggt
     5221 ctttacaagg gaaagctctt ttatatccct tgaaactgtt gcctgagtga ctttcagacc
     5281 atgcctttta agcgcttcac gaagttcctc ctgagtgccg atgttttcgt ctttgacaat
     5341 ctgcattatc aaattctgac gctgattttt catatgccgt cctccgctga ttccttcaac
     5401 ggctgcataa gctttttgtt tattgacgag aaaaagctgc cgccgcttat atctatgatt
     5461 tcaacaaaan nnnnnnnnna ctttgacttt gatactatca cagtgtcctc tgccgaaagt
     5521 ctgaaaacat tattaccgtc aacgctgacg attgcgtgag agccactgtt agtgtgacac
     5581 ttgactttca taacgctgtc ctctgaaagt atcatagatc ttgcaaaaag cgagtgagca
     5641 catatctgtg tgaactctat acacttcatt tcaggttcga ttataggtcc gcctgctgac
     5701 attgaatagg ctgttgagcc tgtggctgta gaaagtataa cgccgtctgc acgaagtgaa
     5761 gaaataagtc tgccgttttt tgatacctca aaatcggcta tcttacaatc gtcagctttt
     5821 gttactgcaa catcattaag cgcagtatat gacttatttt cactgccaca ttcgacattt
     5881 acgtttaaca tcattcggcg gctgagggta tattctccag aaaaaagctt gtccagcaat
     5941 tcaagctgtg aatgttcaag agaggccata aaaccgagtc tgccacagtt tatgccaagt
     6001 ataggcttct caaactcaca gcccttaaga gagcatttga gtatcgttcc gtcaccaccg
     6061 atcgcaacaa tcatatcaca ctgctttaca cactcgtctt cgctacagaa agtgatatca
     6121 agctttccga aaacgtgctt atagcatcta tccataaaaa gttttgcacc atttttgcgc
     6181 agcctttcat atgttgcggt cgtatactca aagcaatgag ttttatcaag atttggataa
     6241 agataaattt tcataatatt accactcttg tgtttagtct ttgaagctat caaaagcttc
     6301 gttaacaaat tcttttatat tgagctgacc aagcttgtca gccgtcatgt cagcgttttt
     6361 aagtagaata agatattcaa gattgccgtc gccgccctta acaggtgaaa ttgttatctt
     6421 ttcagccgca agcccctctg ctcgaaaaca gccaagaagc tcggttatca ctctcacatg
     6481 gtctttacga tctttgacga tacccttttt attgagggcc tgcttgccag cctcaaactg
     6541 aggttttata agaagaacca tttcgccgcc atcgcaaaga caatcgcaaa gggatcttat
     6601 aacaagtttt agagaaataa acgaaagatc accacttatg aactgcacag gtctgtcaaa
     6661 aaaattctct gtcagatatc gtgcattcac gccctcacag tttttcactc tgctgtcatt
     6721 gcacaaactt tcatcaagct gtccgtgacc cacgtccacc gcatagacaa attcagcgcc
     6781 atgttcaagc atacactgag taaatccccc agttgaagca ccgatatctg cacaaacttt
     6841 acctgtcaag tcaagcccga agacatcgca ggcgtgttca agcttaagtc cgcccctgcc
     6901 gacaaacttc atatcgtcag acttacattt caccccgtcg ttctcatcaa ccaatataga
     6961 aggcttgacg cagaccttgc cgtttaccga cacaaatcca ttttttataa gctgtttggc
     7021 acgttctcgg ctttttacgc cgcagttcgt aactatatac acatcaagtc tttcactcat
     7081 ctttcaaaaa gccctcaact gtatcagcca ttttctctgc tgaaaaacca agctcgtcaa
     7141 gtagcacatc acattttccg tgcttcacga aaccgtcaat agcaaactgt gccacatttg
     7201 ggcagataga agcatacttc tctgaaatac ttccacagta ataagcttcc tcaaagaaca
     7261 gcacagcatc gtaggctgac attatatcaa ccacctcatt gtctataggg aaaatgttca
     7321 cgagcttaat aatatcgcac tttatatcac gatttctgag tatttctata gctgtaaaaa
     7381 gatcatttgc aagcctgccg tatgtaacaa gcagaacctt tgcgccctct atatgacgat
     7441 aaacataagt tgaaacagga tcagatgtat catatagcga ttgatccctg ccccttggat
     7501 atctcacagc acaaatgcca ctgtcgtcct caacagcctt atgcaggcac agtctgagtt
     7561 cgtcataatt agacggagaa tatatcctca tattcggcac agaggtaaga aaagaaacat
     7621 caaaaattcc ctgatgtgtt tcaccatcat caccgactat acctgcgcgg tcaacgcata
     7681 taactgcatg acaacctcct atagccatat catggataag ttggtcgtat gacctttgca
     7741 aaaacgatga gtacaccgca aaaacaggta catagttcat agaagcaagc ccagcacaga
     7801 aagtaactgc gtgttcttcg gcaatgccta catcaaagaa gcgttcagga aaacgcttcg
     7861 caaaatactg aagccccgtg ccgtatttca ttgccgcagt gactgcacaa agacgcttat
     7921 tcttttcgcc catttcacaa agctcttttc ccattataga agaaaagctg tcagaaagta
     7981 cgacgtcggg attgcccgtt tttatctcaa aggagccgac cccgtgaaac tctcctgggt
     8041 tagcttctgc aggagcatag cccttgcctt ttatagtatt cacatgaaca aaaacaggct
     8101 tgtttactgc ctttgcagcc ataagtccct gttcaagttc ttcaagattg tgtccgtcga
     8161 taggtccaat atagtgaaaa ccaagatctt caaacatggt gctgtgaagt atcatattct
     8221 tgaccgcatt tttggagtta cgaacggcat ttttcacagg tttacctata acaggcgtct
     8281 tatcaagcat tctctctact ctgccctttg tgcgctgata gctctctttt gtacgcatgg
     8341 aggaaagata ttttgccatt ccgccaacat tttttgagat agacatttca ttgtagttga
     8401 ggataacgat gatatttgta tcacttttgc ctgcattgtt aagtccctcg taaagctcgc
     8461 cacctgttga agcaccgtca ccgactacag ctatagcatg gtgagtttta tcaccactca
     8521 gcttcattgc tgtggcgatc ccaagcgccg cagaaacaga cgtagagctg tggccactta
     8581 taaaagcatc atgcactgac tcgtcaggtc tgcaaaaacc cgaaataccg ttttcctgtc
     8641 tgagagtttt aaactctttg agtctacccg taagtatttt atgtgtatat gcctgatgac
     8701 caacgtccca gacgatttta tctttaggtg attcaaaaac tctgtggata gccattgtaa
     8761 gctccacagt accaagattt gacgcaagat gaccgccgtt ttttgaaacc gtatctataa
     8821 gaattttcct tatatcaccg caaagctcct cacactgcgc agtggaaagc tttttcaggt
     8881 cggcaggcaa attcatttta taaatatcag gcttttttat ttccgacatt atatctagat
     8941 ctccaatctt tttatcaact tcttcttgta agcatatcag atgtaagctc tgtgaggaaa
     9001 acgctgtcct caaaatattt aagatgaccc agtgcttgaa ctgtcagttg ttttgctatc
     9061 tgttctgact tgtcaagtcc aaggagagat acatatgtgt ttttatgctg ttcgctgtca
     9121 ctgccgatag gcttgccaag ttcctcaaaa gtgcctgtta cgtcaaggat atcgtcaact
     9181 atctgaaatg ctctgcccat tgcctgagca tagtctgccg caaaggggat
//
LOCUS       DAWEMR010000101         9196 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3132_length_9196_cov_10.834591, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000101 DAWEMR010000000
VERSION     DAWEMR010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3132_length_9196_cov_10.834591"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2440 a         1777 c         1820 g         3159 t
ORIGIN      
        1 gagcattaat gccgccaaaa atacagaaac acatttcttc ataaacaact tctcctttat
       61 aatcctattt ttatatatta taccgcaaaa acatatattt gtcaacaagt gcattgatat
      121 aaagatatag aacgccctaa tgtgtcacgg tgacacacca ggacgttctt tactaaaata
      181 gttaagtttt gagtatttat gtgttaaact catatttttt attttatatt tatagaaata
      241 aagataaaag ataatatgtt ttttgtcttt tttatagtga aaaaaacttt ttttagaatt
      301 gttagtgaaa aatttgactt ttattttttg gtgtgatata ataaaatttt cgcccaaagt
      361 ggaaatttag tgaaaaatgg tcgatttatc acattttttc tgaaaataat aaaaaatcgg
      421 tttttgttcg tatatgcgtt tgacggcaag aaaattttgt ggtataatat tacttggatg
      481 aggaaataaa aagcagacct gctttcagat attaaaggaa aggaggtaat tgccaagtgg
      541 atgtaccaat tttagcactg accattttgc ttgtttgggt tttgggcaac attataaaag
      601 agcaaaaaaa ataaccgtgc tagcctgtca aactaaacgg ttattttaaa taatcacaga
      661 caaccaactt agtaagttgc gttgcctcat ctatgtttaa tatagcacaa tttgagttga
      721 ttgtcaacat tgttaagact tctcagggcg tttatgctct gggaggtctt tttatttatg
      781 ttatgtacaa tgtgaaaaat tatgaatgtg tcacggtgac acattcatat ctttcatgta
      841 atcttcaata gtgttagcaa cgttaagctt ctcttgaaat gttaagtgag tgtagatatc
      901 gctcgtcact gccaaactgc tgtgtccaag ccatgcagat acggctttca tgggaacgcc
      961 atgctgaacg agcagggaag cacaagtgtg gcgaagatcg tgaaagcggc atctgaggtg
     1021 catttcgttc attagtctga tgtaacggac agaaagctca tcaggctgta taagaatgcc
     1081 cttgctatca acgcatatgt attgtaaata ttgccgtgaa tagctgctcc tgttagctgc
     1141 ataagacaag cactgcttgt gtttgagcca tttcaagtag ctgtatacac tgtcaggcag
     1201 cggaagtgtg cggcggcttg accttgtttt aggcacgtct gaaaatataa gcgtagtttt
     1261 atggtttttg acgcatttga ccacagtatg ttgaatgcac atcgtgtgct gctcgaaatt
     1321 gatatccgac caacgcagcc ccagaacttc actacgccta agtcccattg ttcctgctat
     1381 aaacacaggt gtgtaaattg cagtgtgttt tattttgctg agaacaagat caaattgctc
     1441 tgtgttgaga aagttaccac agaacttgtc gcaggaggga agttctgcaa gaagtgcggc
     1501 attttggtct gtcaatccgt tctttacggc aaatttcagc gtcatcttga tgtttgaatg
     1561 atgtttcctt atggttgagc ctgataaacc tgattgaagc tttgtgaaat aatattcttc
     1621 aagcactgct ggcgtgagct gagaaagcat tgtaccttta ctgcgaaaat acggcacaag
     1681 gtggtgattg acctgggtgc agtaagcttc atatgtgttg gcttttacag acattttttt
     1741 gttgtgcagc cacgtttcaa gcaggtcggc gaacggagtg tcactagctt ttttagccga
     1801 caacttgtcc ttaagctgtt ggttttctcc tgcaagctgt atcacaagtt tttcaagttt
     1861 tgttttttcg tcaattaatt cttcgatagt cataataatc cccttttttt tgatataata
     1921 aataattata ttacaaaaac aggcattttg acagcttgaa ttgatatctg tctgaaagaa
     1981 aaagcgaatt tcacataaaa acaaaaacac actttaataa ctttgataat aactaagtat
     2041 ataaaccaaa atcaattatt tgatttcaga cagataatca agaatgggtc agttatagac
     2101 agttcatttt acccactacc cctttttaag aaaaaaatct ctaaaagccg catttttttc
     2161 ttaaaaccta ttgacaaagc cgaacttttg ttatacaata aacttgtgtt tttaatgggt
     2221 gtgttcattg taaagcaatg aatgctctgc ctcctgcttg agcggcgggg ggcttttttt
     2281 attctgctgt gaggaacata tatgcagaca gtatatgtgt tgtctgtaaa atcacctgca
     2341 cgagttctga ttttcttctt acgaatgaat ttgcctttta caagtttctc tatcgttctg
     2401 agaacgtcct gacggctcat gcctagaagc tgagatagat cattatagct ttggaaaaaa
     2461 ctcttgtgcg agctgtcaga cagcttacag cacattgcat aaactctgaa ttcatttcca
     2521 ttcaatctgc tcataagctt tttatccatg gtgaaatatt tagaagctgt agatatggct
     2581 tcaatagtat atgtatatgt accaagcttt ccaggagcat ttgtttggcg cttctgggac
     2641 ttgataaatc cactcttgag cagtgagctt gccgcacgct tgacagtgga taccgaaaag
     2701 ccgcatagtg acacaagagt tgtctgcttg actgtgatct catagccaag cagatttctt
     2761 tttgtattct tgtggataag actatagaat acacaggcaa gcttcaagtc tgatgcctgt
     2821 gtaaaacgtg taacaaagct gttaggtatt cgcatttttc aattcctcat tattaatgta
     2881 ttgttccagt gccgcccatt catcggcaac aaaattgctg cgtatatcag gacgtttctg
     2941 tattttcttt atgtagcccc ttgcagcggc atagtttttc ttttcaagca ggctgctaag
     3001 ctcatctcct gcaagtccat tgaccttata aagcaatgcg gatatatcca catatgggag
     3061 ttcgctttga ggagcaggga agtcattagg gagataaagc tcgtctgtgc tttcatcaaa
     3121 gccgtcatct tcctcttgaa agccattgtc tgaataagcc tgggcaaata tttttcgtgt
     3181 ttcattctta cgaaattctt tgagctgttg gatatgctgc tgatactcta tgacctggcg
     3241 ctgcgcctga cttgcctgtt tttcaagatc caatgcagct ttatacataa gctcagaaac
     3301 cgctccgcct gcagccttta ttttctcaat atcattaagc tgcatatagt atttgttcat
     3361 agcactttct gcattatttt tcagcttatc aagtctatac tgattgccta taacgcccaa
     3421 actgtcccaa agctcatcat atgggtaaag cacagcagag aggtctatgt tatggtttct
     3481 tataggcaga ttttcgacag gtattcttgg cggcttattc tcgataaggt gataaggcgt
     3541 gatattagtt cgtattggct cacaacgcat tttcttaact atggcagtgc cgaaaggaag
     3601 cttagacaac tgtgttgtgt cgagcagagc cttgcggtca acgtgttcgc cttggtgatc
     3661 gataaagcca tataggctgc cgctgaatgt aagatagttt atggtctcgt ttcctatcat
     3721 ttcggaaaac gccttgttag tgtttccgtt aagagagagt atgtatatct gaatattgca
     3781 agcgcccttt atgccgtcct cttctttatc atactttttg agctgaccat agtcctgcag
     3841 aaagagattg aacagaatgt tagctcctgg agaagcattc attttgctgc tgaggtcagg
     3901 aagctttgtc atttgaccaa actcatcgta gatatactct atttttcgtg gcaatttgcc
     3961 gccctggtat tttcttgcct gctttgcaag gtacataaag gattggtctg caaacattga
     4021 agctattaca tagcgtgatt tttcttcata tggcacaagc atatatatta tgcaaggctg
     4081 atcttcattg ataaggtcag caaagtttat ttcattttta gatgtcagct tttgaacacc
     4141 catatccgtc atgaagatct caaggtctgc ggagagaaca gtgtatattg aagaacgtgt
     4201 atcgccctgg gagaactttg acgttgaata ggatgttctt gcaagacagc ccacaggcag
     4261 cgcattgaac agctcatcga gagcattgac cattttctta ttgccgcttt cgtcctcgat
     4321 cactgtatta tatacgccaa attcagtgaa gaatgttgtt atgctgtaca tattaacgtt
     4381 tgacatatta cctttttcat agcctttatc aagaaaatag tatatcaggg cgcttaaaag
     4441 tgaccttgca gagtctgtcc atattgcttc actttgtaca tcgtctgtaa gacagtgggc
     4501 aaacgagctg acaagcttag aggtttgtga aagatcgttg cttccgctcc gttttgcagc
     4561 gagatattcg tcaatgataa gttgtagagg attccaacaa tcagatcggt ccgtatcgat
     4621 aagattaaga gtgactatct tatatccgtt atttgcaaaa tcctcatatg tcagttcaag
     4681 caactcgcct ttcatatcgt ttatgataag cgagggctta ttttttgagt tagccataag
     4741 ccgcatactt ggcagcacat agcattcgcc tttacctgaa cgaggagcgc ctactatcat
     4801 agtgttgtat gtgccagttt caacatagta cataccattg ctctcaccaa tgagcatacc
     4861 agcttgttcc gctgacttta tattatccat agggactgcc tgaaagtgtt caagaagctc
     4921 attatcttcc ataaatttat tatcaccctt gatgtccttg ctgtcattct tcatacgcca
     4981 cagagtatgg agctttactg ttatacacaa gcctactacc attgccagga taatttcaaa
     5041 aatgaacatc ataaatcctg acggaacaat gatgttccac ggcataaagt gagtgcttgt
     5101 gtattcacca gagcgtccgt taaggcttac cctgcgcata cttttaaaga tgtaaataaa
     5161 aatgctgtta aagatcacat acagtatcga tgtagctatt gcaaattgtt ttattctgac
     5221 atttcggcgg tctttgtctt tttcaatagg tgttttattc accgcattta aaatacgcat
     5281 agcaagatca tagatccaac caaatacaaa aagtattttt gcgatgataa gattttttat
     5341 gcgcttaagt ttttcaagat ccatattagt tcccctttat atgctatgat attgttcttt
     5401 tgctttcgcc atttcctgct cgatctcaaa ttctctcata agacgtgcgg tttttgcttc
     5461 ttcagattta agggcgttgc gcagcatact tgatgattta tgaagaagtg tcctctgcgc
     5521 ctgcatacag cctgcaagtc tgaactcaaa tggtatatcc gcttttttac cgcttattat
     5581 ctttttagca atgtcattgc ctgagcgtgc tgactttctc agccattgat atgctgcttt
     5641 cttttgacca tcacgaagca gcattaaacc ataagcgagc tgtccactgt tgttgccatt
     5701 taaagcgcac tgacggaagc acttttcagc catttcgttt ttattttcag acagatacac
     5761 ttttccaaga atatactgtg catttggatc gttgtattca aacgcagaac gcttaaggta
     5821 ttctttagct tttgtattat tgaatgagct ataatatttg cccagagtgt atatcgcagg
     5881 ctcaaagtcc ttatcggcgg ctgatgaaag gtagcgtatt ccttttgaaa cttctgttga
     5941 ttttctgcta tcaagaagaa ttttccctgc agcatacatt ccgtaaacat tatccttgtc
     6001 tgctgctttg atgaaatact gcaatgcttt tcttttgtcc tgaaatgaaa gatacaattt
     6061 accaaggcgg tatgctgctg ttcccataac atcatcttta tgctcagcag cgtgcatgag
     6121 gtgcttttct gcaagagcat tgtcattgaa ttgctcctga tagagttttc cgagagtgta
     6181 tgctgcatag taattatctt ttgctgctga acgttccaag tatgatgccg ccttgtcata
     6241 tctctcttgt gaaagataca gcttgccaag actatattca cacaatcctg ttttatcctg
     6301 tgcgttttca gcacccttta tcaggacttt ttcagccttt tgaagatctt ttttgttgtc
     6361 gctctgatag agcttaccga gcatatattg tgctttatag ttattggagt ccgccgagag
     6421 cagcagatat ttttcagcac attcaaggtc tatatccgtt cctttgccat tcagatacat
     6481 catacctatc ttgtagctga tattatcgtc atggtttttc gtgaatactt tctctaaacc
     6541 atttaaagct tcagcgtaaa aattatgcgc acttgatagg tctttttcta ctccttgtcc
     6601 cagttcataa gcttgtccta cggcataact tgcgaacggc attccgcctt tgctgcttag
     6661 tgagcgcata taatagtcaa atgctttggc atagtctgtt tcaacaccag agccgtattt
     6721 gtatatgttt ccgagagcaa aataagcata tttattttca gacagcttat aatgctctat
     6781 ggctgagtta tagttctggg taactccaag cccatattcg tacattttac ctatacgata
     6841 gttgagatag gactttgtcc aattatcacg tttatcatca ctcatgctta ctatggacag
     6901 cagcttgtga aaatcctcaa aggctttgct gtaatattgc tgacttaatg tatcgccgtc
     6961 attggatttt agcatttggc tgtcataaag cttgcctatg tcatatgttg cgagaatatt
     7021 tccgcttttg ctttctattg agagtgtttc aaaagctttt tcggggtctt tctttatgac
     7081 tgctgatttg ttctgttcat ttccgtacat atagtcgagg gcaagcttat agttcttgct
     7141 ccaatctatg cgtagacatt ccactgtctg atcgctttta tcattttcag acagatacgc
     7201 ttcaggctct tgcggaaaaa tatccgtgct gtagtcagga gcatttggtt catacaactc
     7261 atcataattt cttgaagata ggtacatttc caaatcatca gggatatcag agttttctaa
     7321 agagtcataa ggcatagcat cctcaaaatc atctttttta ttttcagaca gatacacttt
     7381 tgatttctgc gaggaaatac cgttgttgta gtcagaatca tttttctcag aaaagccttc
     7441 gctttctttt tcagacagat acgttttcgt ttttggaaag taagaagtat tatcaagatc
     7501 tatatgggaa tgcagatcct caggtggttc ataattgaca ttctttgtgt tgtagtattc
     7561 acggcagata gaaagaaatg agtttcctac tctggagtaa aaacctgacg gatcgtgaag
     7621 ctgatgttct ttatactcag cccaccttga attttgtccg tatctgtttc ggaacattgc
     7681 ttgtgtgctg tcgagcttat gaagatatgt gtcaaacatt atttgcaaag tttgattttc
     7741 tgatatgaca gatctgatac aactgtctat ctcgtttttt gtcacatcat tttgttttgc
     7801 ataccaccac tgcttgccac tgttttttag gtcatcagga agcttgtcga taagtgcgtt
     7861 tattttttga atactgcctg caaattcgga cttgatatga ggtgcaagca cgtttctttg
     7921 aaattcagta aggttagcag cggcggcact tttgtcaatg aagtgcgtca tttcagtttt
     7981 caaagcgttg aaagctttct gctcgatatc gtcttgacct ttgtttttgt atgtaatgcg
     8041 cctatcttca agtcggtgat attcaagaag ctgataatgg atatgtacat tacctgtatt
     8101 gacgtgaatg ctgccgaccc agtagcagtt acttgcatcc agcttttctg atttgtctat
     8161 cattttgtta atgccttttc gtgcggcttc aataagtgcc tggcggttga gctgattgcc
     8221 gactatgaaa ttattctgcc gcagatatgc gttatcaaat gagataacac ctgcatattt
     8281 tggacatccc tcagaacggc tgacagcttc ttgttgacgt atcttgtcta tatcggcagg
     8341 agtcagactg tcttgtgtac ttgtgaaagc acctaatgag cgttcagctt ttatgttttg
     8401 agcctgactt ttctcgtttg gcacaaattc tgaatataca tactccattt cgttggcgtg
     8461 tttgttcggc tcaaaagctt cagggcggag catatagtcc agaaaatcga atatcttgtt
     8521 gccaccgtca ccgcttattc gccacttgta tacgaatttg catttgtgaa atattcctgg
     8581 tgtcattatt agtcactctg ttcgtcttgc agggagtctg cacccttatc aactgagcgc
     8641 tgatgtacga tgctcttgta gttgtcattt gatcgtgaca tgacgtcatg cggttcgctg
     8701 tcactcagac gcttgtcggc tgagtggaat tcctgcactt cgtagaagtg caggagcgta
     8761 ttaattccgt cacgttcctg atatatcatg ctgttatttt cttttgcaat cgtcagcagc
     8821 ttttcaatgt tatcttgaat gtttttcaaa cattcatcgt ttggtgtttg tttgatgtta
     8881 ttgtaggcac tgtctatcag aaacgcaaga caattgctgt atgttttaat tccgttcagc
     8941 tccattatct ttaaaaacct ttgataatgt agttctgaaa aagacagtag cttattgata
     9001 ggtttgcttt tcattttttc atccaacctt tccgtgtagt ttgattttat tttgtgatat
     9061 ctaagttaga ggtgtgggtc aagagcataa aggcacgata acgtgcctgc tggaaacctc
     9121 ggtagaaccg aggtttcagg tggggtactg tgatatcatc gggtcttttt ctatatacga
     9181 tttatatacg attttc
//
LOCUS       DAWEMR010000102         9131 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3167_length_9131_cov_10.650397, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000102 DAWEMR010000000
VERSION     DAWEMR010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3167_length_9131_cov_10.650397"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3192 a         1725 c         1962 g         2252 t
ORIGIN      
        1 cctttcatct taaagtataa acatgataaa tcaaatagtc aagaggtatc tatgaaacaa
       61 aatcatgaat ttattaattt cagactattg atatttttgt tattcggatt tgcaggtgtt
      121 attacaagca ttcaaatact atttgaaact aaccaagaaa tgcgtataaa aatatatgtc
      181 atttggtttt tgataaaagc actaataata attgcaaata taaagtacat aaaaaaatac
      241 tttcataagc tgtccgtaaa tggtgatagc tgttttaact atactgaaat gttgcacaat
      301 acatacttaa ctagctgtgt tgtttcagca ctaggactat tagttttttg ttatacataa
      361 gtgtgtcaca gtgacacaca acttaaaatc gtaaacccga gcgtgtgaag aatttcatac
      421 gctctttttt tatataaccg aaaggagaaa tatcatgaac gctgaaacgc caaagaaatc
      481 taagtttgat atcttggaag aaatcatgat aggatttcaa gatgattacg aaacagacaa
      541 tgctcagacc cattgggtaa aaacctacaa agggcggcga gagcgattaa ttcagctgca
      601 agaggaagaa tgtgaactac aagcgaatgt aattgatgac gctcacgatc taggatttgt
      661 tgtgggcgta agatatgcgc tcaacatcat ggatatgaca gatacaggca agaataaagc
      721 gcttttggag cggctcttaa atattcttga aaaataaaag aggggaaact taaatggata
      781 agcacgataa gtatgaagcc gtaaaacaag catggtttga aatcaatgag cagctgagga
      841 acaataagca ggaatacatt gattatctca aattttctgc aaagatgtat aagcagccgt
      901 attcagacgc tgtgctagta tatcagcaga atagaaacgc aacaaagatc gctgagtata
      961 agaaatgggg acgtctgggg cgatatgtaa gacgtggctc tcacggcgtt gctgtttttg
     1021 gcaaaaatca cacttgcaga tatttatatg atgtcactca gacaggcggc aaagagttgc
     1081 ctgagatgtg gacacttact gaccgcaata cacctgagct gctcaatacg cttaataacc
     1141 gatacaatat aaacgcagga tcggtcaaag aatgtatcga agcaatgaca gcaaacagta
     1201 ttaattctgc gcctattgct gagtcagtac aaaatgcaat atctcaaatg ggacttgaca
     1261 caaaacaggc tgaggtatac cgccattcac tggattctgt ggtgcggttc gttgtttctc
     1321 aaagatgtaa tctttcaggt aaaatagatc tgcacgccga tgttgatgta agcgctcttg
     1381 accttatcaa aaatgcaaga gatatgattc ttttcagcag agctgttcag cacgctgcca
     1441 aagacactct gtttgaaatt gaaaaagaag ttatcgccat tgaccgcaaa aacgaagctc
     1501 ttgacaatgc caaaaacgca aagcaagaaa gcacgtctga tagggttgac gatatactcc
     1561 aagaccttat aaagcttaag ttttcgcaag aacaggtact tgcattaaaa caggctgtgg
     1621 tggcagctgc acaaaatggt gaacataaca tacggcattg ggtaagcgtc cgatattcgc
     1681 ccgatgagct acagcatata tcatctttat tatctgatta ctatgctcag agtgtcattg
     1741 agcaaacact tgatccaaag ggcatactca atgattacta tacatatgta aatgagctca
     1801 atggtcggct gaagctgtca gatatgttct ctcaccatga ttatagtgat gaacaaaaag
     1861 ccatgctgca gcagggtttt tctgagggaa tttctattac tgttcttaac gaggtcgatg
     1921 aacggctaac ggtagacgag ataaaaacat tctttgagat gtttcatcag gcagttgacg
     1981 gacagataga tccacatgat gttcagatat atcttgataa agctgtaata gagcattcaa
     2041 agcagaacgt tcaagcggtt gaagtccagg acaactctgc cgacacaatc tcagcagatg
     2101 ttactcaaca ggacaaaaaa atagaaccat caagaattat aggtttggct gaaaaccagc
     2161 tacgccctgc tgttcctaat agctctacaa gcagtataac agactcctct ggagttgtca
     2221 agagtgaaaa aacatttgct gaacagataa atgatgtttt aaatggcgta ttcccaaaat
     2281 actcaaatct aaaggtttgt gatacaccac aaattcttgt ggatatcggc tgtgatcggc
     2341 ttcctatgtt gtatacgcaa aggcatttaa aagacgctct acatgaaaaa tccccgaaca
     2401 atgcacattg gcacggctta acactggaac agatatgtaa tatcccacat cttttagaaa
     2461 gccctgccat aataatggac tcactttctc caaacaaaag tgctgataaa agtatagtaa
     2521 tttgtttaaa taacgttgat aatgataatg caccgataat cgtaagtgtt aaaccaaatg
     2581 ggcaaggcat ttatgaaatg gaaaatgtat catcaaactt tatcacaagt atttatggaa
     2641 aagaaaatgg gtttgcaaac tatgttgaac gtgctgcaaa atcaaataat attctgtttt
     2701 gggacaaaga aaaaagccaa gcactgttct cgtgtcaaag gctccaattg cctgaggcga
     2761 taaacaatct tgactctaat attattatac accaaagtcg caatattgtc aagagtaagc
     2821 aacaagaaaa tagtgccgat ataagttcaa atgatgttaa atcattcact actttatccg
     2881 agcctacgat aacctgtgaa tggagcgaaa gcaactactt tgaggacggc aagacctaca
     2941 gtgttgcaga atttgacacg ctcatggaac aggctgacag tgaaatggtg gcaggagcaa
     3001 aggctgcaat agagaagtac ggttcagcaa aagcatggta tgaagcagac gctgatgacg
     3061 aattctcaca gtttatgggc tatgataagg tcaagtttac tatcaatatg cctgacggac
     3121 aaacgatcac tgaacgccag gacgcagggg acggatacgg cggcgttatt gattttctga
     3181 aaagatactc aatatacaac tcgatcgtac ctgagcttga agctgcaaaa gccgcacata
     3241 tggcaaacct agtcgatgaa gtgcctacac ctacaaatga aacacagcct gaaatagaca
     3301 agcttttggg caggcttaag cttgattgtg attactatct gaaaacaggt gcagaaaagc
     3361 acttgtgggc agaaacagtt gaaaagcaga tagctaagat caatgagctg tatgagaaaa
     3421 tgcctgaaaa cgcctacttc accagagaac agattgatga ttatgaaaag aaaatgctgg
     3481 caatcaaaaa tggcgaagtc gaaaaaccta taccatatga aacgcctaca attgctaata
     3541 atgttctgtc tgaagaaaaa aagacaacac cgcctgtttc tgaaagcgtt cctatatctc
     3601 ctacgtctga tgaacagatt tcactctttg aaacacctcc tgtttctgaa accgccacta
     3661 tacctgataa agatgtcaaa accgaaaagg tagaaaagcc aaagaaaaat gtcaaggcaa
     3721 gcacatacca ctttgaccct gataatgttg ttgtgggcgg agctgctgct cgatgtgacg
     3781 caaatatagc tgctattgaa acattgctga aaatagagag tgaaaagcgg cttgcgacac
     3841 ctgaagaaca gaagataatg gctagatact caggttgggg cggaactgct caggcttttg
     3901 ttgcagataa tgagaatgtt tctgctgaca gttggagagc aaggcagact cgtttaagag
     3961 aacttctgac cgcagatgaa tataccgcag cacggtcatc tacacttaca agcttctata
     4021 caccgccaga agtcatagac agcgtatatc ttgctttgga aagatttcaa tttgagggtg
     4081 gaaacatcct agagcctagc atgggagttg gaaacttttt tgcaaaaatg cctgatgaaa
     4141 ttagggcaga ttcaaaactt tacggagttg agcttgacag catttcagga cgaatagcca
     4201 agcagctcta tccagaggat aatatccaga taaaagggtt tgaaaaaaca acattcaaga
     4261 ataacagctt tgacgtggtc ataggtaata taccttttgg agattatggc attgcagaca
     4321 aagcctatga taaataccac ttcaaaattc atgactattt cgccgcaaaa gcagtagata
     4381 aggtaaagcc tggaggaatt gttgccattg tgacttcaaa attcacaatg gacaagaaaa
     4441 atgataaagc aagaaagtat cttgccgaac gctgtgacct tttaggcgca gtaagacttc
     4501 cctctggaac gtttaaagac gcagacagtg ttacaacaga tattatcttt ctgaaaaaaa
     4561 gaacaacaat gacctctgtt ataccagact gggtacacgt ttcagaaaca gccaacggca
     4621 ttccttgcaa tcaatatttt gtggataact ctgatatggt acttggtact atggcgtggg
     4681 acgagcgtat gcgaggaaaa tatggtgcag acagcaaggt aacgacctgc tatgcaaaca
     4741 gtgacactcc ccttgcacag cagctcataa atgccatttc aaaaatagac ggcgttattg
     4801 atactgtgga aactgagctt gacaatggga cagctgagat actccctgct gatccaactg
     4861 tcaggaactt cactcatacc attgttgacg gagatcttta tttccgtgaa aatgaagtaa
     4921 tggtcaaggt cacagaaaca ggcaaatcgc ttgaacgcat gaaagggctt catactctaa
     4981 ggcaagcaac tatggagctt atcaatgccc aggctgacgg ctgcactgat gaacagcttg
     5041 cagagcttca gaagaagctc aacagcacat atgacaattt cagaacaaag ttcggaaaca
     5101 ttacggacag cgccaattca cgctgctttt ctaatgatga tgactacaat actttagcag
     5161 cacttgaagt tgtcaacgtg gaaaataaga cagttgaaaa ggctgcaata ttcacaaaga
     5221 gaacgatact tccagatatt ccagtttcaa aagttgatac agcactagaa gcacttcaag
     5281 tgtcaatgga cagattgggc aaggtcgata ttctctatat gtcacagctc acagacacta
     5341 cgcctgaaaa ggttatcact gaccttgctg atgaaatatt cagagatcct gctaaagtta
     5401 aagacaatga cccatattca ggttatacgg agtcatctga atatctctca ggcaatatcc
     5461 gtgataagct gagaatagca agaaattatg cggagcatat cgacagcagc tacagcaaaa
     5521 atgtgaatgc cctgcttaaa gttgtaccaa aagaccttga tgcaagtgag atttccgtaa
     5581 ggatcggtgc aaactggatt gatattgcag attacaacaa gtttctgaat gaatatgcaa
     5641 aagcaaatac atacttatac cctgtcacaa gaacaacgct tggcgaatat aagattgagg
     5701 gaaaatatca agaccattct attgccgcaa atcaaaccta cggtacaaac cgaatgagta
     5761 gttatcatat ttttgaaaat ctgcttaatc agcgtgatat tttagttcgt gacagacgag
     5821 aggaggacag taaagtatgg tatgagataa acactaagga aacacagctt gcaaaagaaa
     5881 aagctcgtct aatgaaggag gcttttaaaa actggctatg gaatgatatg ggcaggcgtg
     5941 aaaaatacgt tgcaaagtac aactatctgt tcaattccat aaggggcaga gagtacgacg
     6001 gctcacatca aacctttcca ggcatgaata catcaataac gctgcggaaa caccaatcaa
     6061 atgctataat gcgtgcaaag cttggaggaa acacactttt cgcacatgaa gttggtgcgg
     6121 gaaaaagttt tgaaatggtt gcaactgtaa tggagaagaa acgtcttggt cttataaata
     6181 aagcctgcgt agttgttcca aagcacctta cattgcaaat ggcaagtgag tggctgcggc
     6241 tttatccaaa tgcaaaactg cttgtggcaa aacctgaaga ctttaccaaa gccaatagaa
     6301 agcgttttat tgcaagatgt gttactggtg actatgacgc cgtgataatg tcatttacac
     6361 agtttgaaag aatacctatg tcagacgagt acaaagagca gttcatgcaa aaagaacttc
     6421 acgatatcat gagtgcaata tccgaagcag gaagaaaaga taaatcatca gtaaaagccc
     6481 ttgaacgtca gaagaaaaac atagaggaaa ggcttgagaa gctgctttct tctccaaaag
     6541 actcatcatt gtgttttgaa aagctcggct ttgactatct tgtatgtgat gaagcacata
     6601 actataagaa ctgctttgtt tctacaaaaa tgtcaaatgt ggcaggcgta caaacaacag
     6661 cagcacaaaa atcagaggat atgctgatga aaactcagta tctcaacaac aagtacggct
     6721 gcaacaatat actttttgcc acaggtacgc ctataagcaa ttccatggtg gaattttacg
     6781 ttatgcagcg ttatctcaga ccagatattc tcgaaaaagc aggattgcag aactttgacg
     6841 attgggcaag cacatttggc gaagtcgtat cacaattaga gataaagcct gcaggaaacg
     6901 gctttcaaat gaaaaatcgt ttcagcaaat tcgtaaacat acctgagctt atgcagatgt
     6961 ataaggaatt cgctgatatt caaacagcgg atatgctgaa actgcctgtg ccaaagcttg
     7021 aaacaggcaa gccgatagta gtgacctcaa agccagacga acgtcaaaaa gcatacatga
     7081 aagagcttgc cacacgttct gaaacgatac acagcggcac agttgaccca cggcaggaca
     7141 atatgctgaa aataactcat gaagcacgct tgctcggtct tgacactaga tgtattttca
     7201 aagacgctca gcctgcacca gacagcaaag tgatgaagct tatcgataac ctcgaaaaga
     7261 actacaaaaa cactatgaca gaaaaaggcg ttcaaatagt attctgtgat atcgctatca
     7321 acgaggacga aacacacttc tccgtgtaca aagctataaa gcaggcgctt atggagcgtg
     7381 ggataccaga gaaagagatc tgctttgcag gcgacgcaaa gacagacaag gcaagggacg
     7441 aaatgttcaa aagcctgcgc aaaggcgaaa aacgttttat catcgcaagt acctcaaagc
     7501 ttggaactgg cgcaaatatt caggatagga tctgcgcaat tcatcacctt gatataccat
     7561 ggaagccctc tgacctcact cagcaggacg gacgtggaat aaggcaaggc aacagatttt
     7621 cgcaagtcgg aatatatcac tatcttacag aggaaacctt tgacgcatac atgatgggca
     7681 ttataacaaa caaggcaaaa ttcataaatc agatcttgac ctcaaaaagc cctgcaagag
     7741 tttcagaaga tgttgacgaa atggtactta catactctga aatgcaggct atagcgagcg
     7801 gtaatcctat gataaaagag aaaatacagc ttgacaatga tgttgcaatg ctgaaaaccc
     7861 ttgaagcaga acacaagaag tcaatttata aaatgcagga gttggcagag aaaactttgc
     7921 cgaagcagat aacacattac tctgaacttc tcgcaaaatc caagagtgat atgtctaaat
     7981 atcaggaaca tcaggcgtta aacaaggagt ttgaaatgac aataggcggc gtcagatacg
     8041 ataagagaga aaatgcagga gaacaaattg ccgtggcaat ggcaaaatgt actgccacag
     8101 gtgagcctat agagcttgga acgtatcgtg gttttaaagt caccatagaa agaaatccgt
     8161 cagcaaatac attttttgag cttgatacgc cttgtatcgc tgttctccac ggagagctta
     8221 cttattcatg tgacgttgcc acagataatg gcgtgggaaa tgtcagaaga attgaaaatc
     8281 ttgcaggaat acagataaat cagaagctgt gttctcttga agaacagctt gaaaaagcaa
     8341 acaaggatct ttcagaagct cagcaaaata tgctcaaacc atttgagcat ggtcaagaat
     8401 tagccgaaaa gacaaaacgt cttgaatatg tcaatgcaca gctcagtgga aattcgcaga
     8461 ataaaaacca cactcctgaa agtgctatag aatttaggtt tcacaacaat aaatatcagg
     8521 cattgatcgg cactaatgaa aaatcagaat ggtgtgatgt agtatcaaag ggtggcaagc
     8581 tcatcgcttc atcagataca aagatctata atctgtctga aaaagaaagc gatcaattcc
     8641 gcagctatgt gtttaatggc ggcaaaactg acacaagcaa gaataattcc ttactggaaa
     8701 agcttaagcc aaaggcgctt aaattataaa acattgagag gaggtgagga tatatgatag
     8761 acagaagata cgaatcaggc aaaagctttg tcatgtactc taaagaggag cttgaagccg
     8821 cacagcgaac agatatggtc acatttctcg aaagccacga gggtttcaac tttaaatcgt
     8881 caggcggttg gtatgtaggg atagagcatg acagtcttaa gatcaatcca gaccgctata
     8941 cctggcactg gtattcaaga gatctttacg gaaaaggtgc tatcgactgg ctctgcaagg
     9001 ttgacggata cgattttaaa gaagctgttt ctcgtctaat aggcaacgcc agtatacggg
     9061 cgagaggagg tgagggtata tgatagacag aagatacgaa tcaggcaaaa gctttgtcat
     9121 gtactctaaa g
//
LOCUS       DAWEMR010000103         9094 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3180_length_9094_cov_12.494745, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000103 DAWEMR010000000
VERSION     DAWEMR010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3180_length_9094_cov_12.494745"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8237..8246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8482..8491
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2728 a         2079 c         1549 g         2718 t
ORIGIN      
        1 tatttgtgct aaaaatcacc gaaatcggct taaaatcact acaattgcat tgctctaaaa
       61 cgccgcaaaa tcgggctttt tcgcatttta ctaaagaccg catcaggtgt gcggattaat
      121 tcaattcccg tacggatcac caaagtttaa agctcggaga tagcacattg tgctgtttcc
      181 gagcttttgt tatctgaagt ttcatacctt ccatgttctg ccctactttc ccccgcacac
      241 ctcactcttg agttttttcg ccgccttgaa atactctcgc attttcacgt ccgcagggtc
      301 tatctgcaat gcaaaggata tcctgcccag cagcgaattt atgtacttct caggcgtaat
      361 gtcagcacct ttgaaaaaca ggctttggga aactccgtat ttctcacagt aatatatttc
      421 ctgacgtatc tcccgacgct gtttcgacga gatctgcgcc ttttcgttga ctactatccc
      481 cgttacctgc tgacgttgcg atgaaccgat aagcgtcgtt tttttcatgt tcagcttaaa
      541 acccattttc cacaacgccc tgctcacaag ttgcgttatc tctttggcgt taagcacagc
      601 ttttccacct gaaaatgtca tatcatcaca gtatcttgtg taatttatgc cacgctctcc
      661 acaccacttg cctatcctct catcaaatct tttcatcacc aaattcgata tctgcggaga
      721 tgttggcgca ccctgtggca gtatgctgtt atgagtgcat atatttgtca gcagcgttgt
      781 agcaggctta gaaaatccaa gctcgcacat gaccccatag accttgccaa aatcaatgct
      841 gtcaaaaaag cctgaaacat caagtttaag cactctctgt tgaccaatat gcggctctgc
      901 atttttcaaa agcgaagagt ttttgcaata tgcagtggca tactcagata cggacagcgt
      961 gtaaagatac ctttcaagta tccttctttg aacgagcttg agatattcgt tcggcacttt
     1021 taacaccctg tttttatgcc ctctgccacg atgtattatc accttgcggt aatttgaata
     1081 acactttctc tgatttcgtt tatgatagcg tattcggttg ttgctaagca tataaagctt
     1141 tttcaaagga acatcaagct cctctgagat ccttatgata tcattatcaa tatcattcat
     1201 tttgtcacct caaaaaaaaa aaatgacaag cgtcagcaca agattcaaat gctttggagc
     1261 cgaattttat ttaatggaat taaatccgat tcggtgaaaa agcttttgat aaagctacta
     1321 tttcctgaaa cttccacagc gactcgctgt agttatgcgt tttgcaatat cgctgctgac
     1381 gcttgccata ttttactaaa ccatgttatg tactcatgat aaaagcacct tgtttatttt
     1441 atgagataat tatatcacgc gaaatgcaca acaattggtg catctcgcag agtacaacga
     1501 aaaaaacggc tgtatataaa cactctgata tcattttacc aatttatgca caacacgtca
     1561 atgggttatc ctgacatttt acgccaagcg ccaatatttt gtatgaatac acaaaaaagc
     1621 cacacgaaga tagggaaaaa agccgagcct tgcttttatc acctctcaat atcaccacct
     1681 ctcgagcttt ttccttttta tcactatccc caccggacta tccgtttttt cgtcaaaata
     1741 ttcctcgcag acccaccaat gatactcttt cctgggcagg gtcgcctacg tcatatagca
     1801 aaagctccac tggagaagca attccttctc cttgccgttt ttcagacttt caagtagata
     1861 gtcctccata agcttcacta tcgtatcata gcttataaat ctcatcttgt tctttctttt
     1921 tattatggac agcctgtggg caagataaaa atgcaaaaca actttccaca tgctgcattt
     1981 gtattctggg tcaagtattg actctttttt cattctcctg atgatcttaa tacgttcatc
     2041 gggatcattc aaaaagtcgc acagcgctga gatattagaa caatcgaaac cgtcctgccc
     2101 actgtccagc ttgaaatatc tccgcccgtc tagtttgtat aaaggcactc gtgctatctg
     2161 cccttcctgc gcctccactg catatgtcca caacctcggg caggatctta tctcatagac
     2221 ttctctgcca ctggtatcct gacctctctg tacattaaga aatctctccc ctatgttgtc
     2281 accgacttcc tgcggagcat tttcagtgcc tgctataaaa atcctaagcc tttggagctt
     2341 ttccacgctt tctcttagct tgtctttaag tgcatttcgt gaaaatctaa tgtcctcgtt
     2401 tccgctgaga agttcccata tggtgttgag ataaaactcc tttctgttat tattccatat
     2461 agtgcaaaca gcgtcaaaaa cacacatatc aaagtatgtc agcggtgctt tttcataagc
     2521 cgtgcagtct tgataccata aggtacaaca gatatcattt ctgcgctgac ctttcacaaa
     2581 agctactctg tagttatagt cctcttgatc gtttcttttg cctgacagcc cgtcataaag
     2641 cccttgtatc tctttctctc ccaaaagcct gtttattttc ttgtctatat ataaccaacg
     2701 atccttattt ttctttatga ctatcatttt ttctcagcaa gcaaaagtcc aaacaaatgg
     2761 acagatagta tgatataata taggcataac aacaaaaagt gaggtaaaat cgttatgcct
     2821 aaaagaaaca cactcaaaag caagctcagc atcatagttt tttagatgaa attgaactca
     2881 aaagacaaat gccttgagtt tctatttgaa ttgcgttttc cataaggttt ggtttgtctg
     2941 gaatgtgact gtacagagta ttatcatatc aaaaagcgtg gtcggggaac taccaaaatg
     3001 aagatcatgg tggctgtaca gtgggcaaaa tgctcgccac tgtgagggat actattccaa
     3061 gcaactgcgc atacatgata ttcgaggagc tgatattagc accgttcagc attgcccgta
     3121 tgcgtgaaaa tttcaaaagg cgcagaagac gtcaacaaaa caccaagaaa gaagtggaat
     3181 aaaaatgcca caaacagaag atatagaata catcaagaaa tatgaggcgt atgattgtat
     3241 caacgactcc gaggacgatg attttgaccc actggacgag ctttacgagc ctgatcctgt
     3301 ggagattcaa aacacgagcc aaaatccatt tacagcaaag gtatttgacc ccgaaacaat
     3361 gcttttcgac cttgccgaaa caaggaagac agaaacaaaa ttcagagaga atatactctc
     3421 tctgatgatg tctgttctca taagctcaga caagcccaac gcactgctct gcggtcctgc
     3481 gggctgtgga aaaaccaaga tagtggagga gcttgcctac aggctcaaaa gcagcgagaa
     3541 catcgtacca cagctcaaag gctataggat atatgccctc cagctctctg atatcgtggc
     3601 agattcaggt cttatgggtg accttgaaaa taaggtcaaa atgctcataa actatatgag
     3661 cgaccaggac gagaagcgaa ttttgtttat cgacgaaata catatgcttt ttcaggaaaa
     3721 ggcatatcgg atcgtcgctc agatcttgaa gcctgctctg agccgtggga aaataaagct
     3781 cataggagcg acaacgaccc aggaagcaaa tctcatcgac agcgaccctg ccttttgcag
     3841 gcgtatgaca aagataatag ttgacgaagc agaccatgag cagacagtgg agatccttct
     3901 cagcatgaac gagcattttg ccaagcacta taacatctcg ttcgctttga gccgaaacaa
     3961 ggcagagcac attgtggata tcgcagacga attttgctac tcaggctcac accgccctga
     4021 caatgccata acgctacttg accgctccat agcttccgcc gtggtgaaga acgcttctga
     4081 caagaagatg aaaattactc tcaccgaccg ccatataaaa gaaacggctt tccgcatgac
     4141 atcaggtcac agcacgcccc acgcctttaa cgagagagac tttcaccgtg acctctcagc
     4201 cgtctgcggt caggaagctg tcatagacga ccttgtcaat gtgcttaagc tccattctct
     4261 ccacatcagg ccgaccaaaa agccttttac aatgcttttt atcggaccaa gcggagtggg
     4321 caaaacagag gtcgcaaaga taatagccaa aaactgtgca ggcgagaaac ctatcacact
     4381 gaatatgtcg gaattctatt atgacgcagg cataaaccgc attataggct cgcctgcagg
     4441 ctatcttgga agcgacagca atgttgaact gccctttgat aagctcaaat caaatcccta
     4501 tcaggtgata ctccttgacg agtttgaaaa gtgcgacaga tccgtgcaaa gactattcat
     4561 gtctgtcttt gacgagggca ttctcaccac aagtcaagga aacgttatag acttctcaaa
     4621 gtccataata atagccacca ccaacgcagg ctgtaccaca aagagcagaa gcattggatt
     4681 caactcagac agcacatcag aaacacaact cataagcgac ctttccgact attttgacgt
     4741 cgagcttatc aaccgcttct cacagaaata cactttcagc gagatatcaa gatcggtgta
     4801 cagaaaaata gttgaaaaac ggcttgcgag cgaaataaaa gtcatcaaaa ggcttcaccc
     4861 tgaactgaat atggactccc tgttcagtgc agacgagctt gcaaaggcag tagatcagat
     4921 aacagccgac acctacaaca tcaaatcagg tgcacgacct gccatcacag cagtaagcaa
     4981 gttcattgac agcaagctcc tttcacactt ctcacgcatg gcaaaaacat acaggccaaa
     5041 ccaaaactaa cgcttcgcca tacaaaaaaa tcaatgaccg cacctttcac gggcgcggtc
     5101 atttttttat tctgacaatt ttactcttat taaacagata taaagaaaag aaagataaaa
     5161 aagctcgaat tatattatta ggatatgtca cacccacaaa atatcacgct aaacagccga
     5221 tatcaaaatg tgatttttgc atttgatgtg cgggaattgg attttttcta tatgatctta
     5281 ccgtcagaca cttcgataac acgactgcat tgttctgcga cttttggatc gtgagtaacg
     5341 ataacgactg tttttccctc tttgttaagg cgtttgaaaa gctccattat ctcggccgag
     5401 gtctttgtgt ccagcgctcc tgtaggctcg tccgcaagta tcatggcagg ctcgttgaca
     5461 attgctctgg ctatagcaac acgctgcttc tgtcctcccg aaagcttgtt gcaaggcttc
     5521 ttagaaagat cccccatacc aacagctttt aatgcttcaa gagctttttc ttttttattc
     5581 tttatctttt tctttgcaaa atccaatgga atcaatacgt tttcaagggc agaaaaatcc
     5641 tcaacaagtg caaagtcctg cattacaatg cctatctttt cattccttat ctgtgcatac
     5701 tgcccctctg ataaattttt taccaaagtg ccgtcaattg tgtactcacc gctctgatag
     5761 ctgtctatgc aggcaaggat atgcaaaagt gtggattttc ctgcgcctga ttttccaatt
     5821 atcgctacca tttcaccgtc tgatatctcg catgataccc catgcaaagc ctcatattca
     5881 ttggctttct ttggattata tatctttatt attttatcaa gctttatcat tataatgcct
     5941 ctcttattca tttatcatca agatctcatt tggtgtattt cgtctcatta taacgcccgg
     6001 tatcaaaagt gagatcagaa tgttcaagat aacgatacat acacagccaa tcaagtgcag
     6061 ccaaccaaaa cttatcactg tatttcccat aaattcgctc atagtaagaa catttgaaat
     6121 acacactgca atggcggctg ccataactgc ggtcataaga tcacacacaa gatctatctt
     6181 gatatatgcg ttccactttg ctccgcagag ataatagata ccataatctc caagtctctt
     6241 ttttatgttc actgccgaag ccgaaactga gcttacaata acaagaacta tcacgcatat
     6301 aagcattgga acaagcttga taagctgacc attaatatag acaagcgaat tttctctgaa
     6361 atcctcaaag tcaaaaaccc tgccataatc cctcaggtat aattcaagct ccttaaaacg
     6421 ctcattgctt acattatcct ttaacgttac tatttgattg ccgcaaggat atattttaca
     6481 gtttatatct ttaagacagt ctgtcggaac tatcagcatc atgttgccat gcttattgac
     6541 attagcataa agatctctga aatcaggctt ttcttttata ctatcctcag tgcctaaata
     6601 tgcagatgaa aatcccagca actgtgagcc gtcagctatg acaccgacaa tttccacctt
     6661 gttcttaaca tactcatact tataaggatc agcatagtca aaatcctcat catcagggga
     6721 catctcccct actttctctt tgtgcataat ggtaactatg tcacccgttt tgtagttgct
     6781 gttttcagtc acaacagcag gtatcaaatc tccgttagcc gacgcattag acagccattt
     6841 tcccgaactc atctgcggcg taaatgactt tatcatatct tcagataaag cattaacgct
     6901 cattctatcg tcaccaagtc cattagatag atcttttgat gacggagaat aatacaagct
     6961 tgtatcatat ccgcttgtca ccgactccac atcatcaata tttttaagct cactgtcaag
     7021 aggagcagta tcatatatat tacatatata tagtcccttg ccatcaggaa agtatttctt
     7081 tacaggagta tacatctcta ttcttgaaag aacagaagaa acagccgccg tcattataat
     7141 aaatgcaatg gcgagctgaa cagcgataaa aatattgctg tagatatttc ttgcaaattc
     7201 ttttaaacca tattttatca tattattttc ccctctcttt catcattttc acaggggatc
     7261 ttttattggt aaacaatatc acaatgttaa ggaacaaata aatagaaata atatatattc
     7321 caaatatggt catatatacc ctgaaattat atgcgacctt gatatactca aaaaacaaag
     7381 taagcttagg aagcagcgca taattgaata ccaatgcaaa tatcacatat atcgtcactg
     7441 atatcatacc tatctcggct atatacatcc gtcttatttt atttgacgtg cagccacaca
     7501 gcatgaatat agcagtctgt ttagcacggg atcttattat gtatctgaaa agcattgcaa
     7561 ggtttattgc agaagctatc gcaacggcta cggacataaa tataacggaa ttgtagaatt
     7621 ttatttcagt catatcgatc gtgtctatcg gaggaaaatt aacattacac gttagaactt
     7681 cgctgaaagc ttttttcagc ttaaaaaagc tttctgtatc gagagcattg tcatcaagaa
     7741 aacttatgct tgaaatagtg cagttatcac ccaacgtttt aaatggcacc tgaaattcct
     7801 cttgtacagt attaccgaat atgcctatga ccttatagct tttgccaagc agttcaacat
     7861 tcttgccgat catatcatct tttgcgccgc taggcagcac gaccagattt tcgtctgaag
     7921 catattcttc cttagtaaaa tatctgcctt tgcgtatact catgttgtcg acatacgatt
     7981 cgtataaact ataatcattc aactcctcat catattgaat gcgcaccgca gacataagat
     8041 acggttgacc gtcgtcaaaa cactcatcaa ttttaagctc gttctgatca aaatttatac
     8101 taaaatacat acccggtatt gacgctttag aattatcatc aagcttgtca agcacctccc
     8161 gaagctgaac aggagttata gaatctgttc caaaataaga caagatcctg ccatcactgt
     8221 cttcctgagt atctatnnnn nnnnnntata tcgccaaatg aaaaagtata gttaaataca
     8281 tacttctttt gcttttcaaa atccttgctt gctttaaaat tctgaaatgc accatgtgaa
     8341 aacaatatta taagcacatc agtaacggcg caaacaataa atagaataaa gataagcaca
     8401 ttctttacgg caaaattttt caggttaata aaaacgtatt tcatttagtc ctcttaatat
     8461 tacttatttt gatttatacg annnnnnnnn ntatccttat cggttttaac ttttacacga
     8521 tggatataat aaacatatga attattcttg tttcttttaa ttccaatacc tgttccggca
     8581 ttttttgttg ttatctcatt tgtctgattt gatataacag cattatttgc tgtatttctg
     8641 cattcaaccg atacctttat gtaacgttga aacttacttt ctgtttttcc cgcagtagtc
     8701 attgcagttg aagaatcgcc cttaatattc cagctcacat aactattatt tccaagcttt
     8761 cctgaataag catatgactg accatatacc tctttggttg ctgacgtagt tgctaatgca
     8821 gtagtaacag ccatagtgct tacaatagcc aatgcaacaa ttcccgtaac aattttctta
     8881 ataatcatac ttatttcctt tctcgtttcg caataaatca cgaattttta attatacaac
     8941 taataatttt atgacctgtg cttaacttag aatatcttca tgctcacttg aatttcatca
     9001 aaaattcaac acagtactat gaagttctaa gtattctgac ttacttccac tgtaggtata
     9061 cacctgtaat tccggacgat atttatgacc tgag
//
LOCUS       DAWEMR010000104         8983 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3231_length_8983_cov_11.244848, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000104 DAWEMR010000000
VERSION     DAWEMR010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3231_length_8983_cov_11.244848"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2409 a         2013 c         1944 g         2617 t
ORIGIN      
        1 tttcgctgtc acgataattt ttttcatttg tgtcttgaca agggggacat tatacttaac
       61 tattgacaaa aattctgaaa catggttagc acattgctat aaccatcatg acacgaataa
      121 tgatatagag gaatttatct ttatgtataa atcaaatccg atacctgttc aagcaataaa
      181 gagtattcat gaaaaacaag tttgttggaa aataacacgt attgatttcc cgaaaggatt
      241 gctaaacgat aaagaacacc ttcaaatttt gttaactgac gcgttcaaag cttacaacat
      301 atccggatgg cctggcgaag aaagtggaca gactaaggta atatatggct atgagtaagg
      361 aggcgtattg taatgttgac gttattggaa gcattagatg agctcaattc aaaatcagaa
      421 agtgaactta cgcttgatta tcttgaagaa cttgctaaaa aagtatctat cgaacctgtg
      481 cctcatttaa cgattaaagg ctctgagatg acctaaaata ggtcttatca gagccttttt
      541 ttacattatg ctttttaacg aaatgtcatt ttaggttgtg catttattct ggcagcagga
      601 cgcaggcaca gaaaccgggt tcgcgccgat agcaatatat ggtgaggtct tttccgatct
      661 cagggctaaa atccttcagc gtgtgctttg tgccgttgag tgctacatct gcgtaagata
      721 ccagccattt tcggtctccg ttgcgaaata cttcataaaa agaccgattc agcatttctt
      781 ccacgggcac accctcaact accgccatct ccttgttgca gtagcggaaa ataaagtcaa
      841 caccgtgacc atcctcatcg aatacaagtt cgataacgca gtaagccaga ggcatttcgt
      901 ccataatact gcacttctcg tagaaaccca acggcgggtc gaattcctcc ttaggcgacg
      961 gaacgtctag cccaagctcc ttacgcagcc gatgtggatg ggctgaatgg cagctttgac
     1021 aagaccaagt ttgatcttgc ttctgcagag aatacaacaa ttgaaaacgc cccaacagac
     1081 acaactgctt ttggcatttc cggaggtgcg atcacaaaag atcagaagat cggaactatt
     1141 acagtaaaga tcacctcaga tacttcagac accactatgg tgaaaaattt ggaggatctg
     1201 cgcggtgtat ttgaaaatgg tggcaaggca aagctaaaca atgatcttaa cggagcatac
     1261 gagatgctga cgctttcgtc aggaaaagat ctggagtttg atctgaacag aaagacgctg
     1321 agcgtcgaat ctatttcact ctcaaatgat ggcaacgaaa cgctgacgct gcgcaacggt
     1381 actatcggct gttatgtgca gatgaatgga agagctgaac aacatctgat cgttgacaat
     1441 tgtactctga atgggttggg cgacaataat aattactcag atgtgacctt gagagattgt
     1501 gtcatcatga aagattgctt taccagctat ggcggcattt ggaaattcga gggagtcaat
     1561 aatattactg gaacaatgaa agttaagaaa gatgtcacca tttccggcga ctttacgtta
     1621 gggacgctta aagttcccat ggttactacc ggtacgccga ccttaaaact gtcgggtact
     1681 attcggatag gcaaattctc ctttgatagt gtttatagag aagaggcaaa gatcatctgc
     1741 ggcgtgggta catataactt taagccggat gaatatgaaa caggcagata tggcggtatt
     1801 caactagcag aaggatgcac cgtcagcgga cctgatgaaa acggcattta caccgttaca
     1861 gcggaataag gagggcaaaa aaattaggca gccgttaccg actacctaaa gaacttattc
     1921 tattgtgcaa tgaccttgta tatgataaat gcccatagtg caagagaaat tatcatcacc
     1981 atataaaaat accaatgttt tgttttgtga cggaaaaatt ccatgcccag aaatcctcct
     2041 gtaaagccgc caagcaggct cagaccaagg agcgttttct caggtatccg ccatttgtgt
     2101 gctcttgcct tgtgcttatc tgcaccaaaa agtatgaacg tcaaaaggct cattgcggaa
     2161 agatatatta taatgtattt cagcatatct ttgtcctcat ataaacaaag ggcggaaaag
     2221 ctccgccctt gtatttttgt ttttttactt gaagtaagct acgccgcttt caaatatttt
     2281 ctgatccttt tcaccaggaa cgttctttga tacgcagcag cccttacgct ctgagtgacc
     2341 cattttacca agaacacgtc cgtcaggtga ggtgataccc tcgatagcct ggaatgatgt
     2401 gttagggttg caatgaatgt caaatgtagg ttctccgtca aagtcaacat actgtgtagc
     2461 gatctgtccg tttgcagcaa gctgtgcgat ttcctctgct gttgcaacga aacgtccctc
     2521 accgtgtgag atagccacgc tgtggatatc gcctacctca cagcctgcaa gccatggtga
     2581 cttgttggaa gcgatccttg tgtttaccat tttagactgg tgtcttgcaa tggtgttgaa
     2641 tgtcagtgta ggggagtcgt ctctcatatc tcttatctca ccatatggta caagaccaag
     2701 cttgataagt gcctggaagc cgttgcagat accaagcata agaccgtcac gatttttcag
     2761 caggtcatgg atagcgtctg taagccttgg gtttctcagg aatgatacga tgaacttgcc
     2821 tgagccgtca ggctcgtcgc caccgctgaa tccgcctggg agcattacta tctgtgactc
     2881 ttttatcttc ttctcgattg cgtcaacaga ctccttgata gcctgttctg tgaggtttct
     2941 tacaacgaag atgtcagcct cagcgcctgc cttttcaaag gctcttgctg tatcgtattc
     3001 gcagtttgtg ccagggaata ctgggataag tacctttggc tttgccacct tgatagcagg
     3061 tgcaactctc ttgtctgcct tgtagctgta cttttcaggc ttttcggcag gctctttcac
     3121 atgaacagga tatacaggct caagcttgcc ctcccaaagt gtctggagct tgtcaaggct
     3181 tattgtgtag ttgtctgact ttatctcgta gctttctgtt gtttcaccga taactctctc
     3241 gtcagtgtca acgccctcag caagctcgat aacaaatgcg ccatagcaag ctctgtaaag
     3301 ctcttctgct gtgagcttgt ctgtgaactt aaagcctatt ttgttaccaa atgccatttt
     3361 tgcaacagcc tcagatacac cgccgtagga tacagcccag catgaaagca ccttgccctc
     3421 agcgataagc ttttctacct tttcaaacac tgccttaagt gaatcgaagt tcacaagctt
     3481 gttgtcgtca tattcaggct tgataaggat caccttagag ccggccttct tgaattcctg
     3541 tgaaactacc ttgtctgcat ctgttactga aacagcgaat gaaacaagtg taggtggaac
     3601 gtctatttcc tcgaatgtac ctgacattga gtccttaccg ccgatagaac cgcatcccag
     3661 ctctatctgt gccttgtacg caccgaggag agcagacata ggcttgcccc aacgctcagg
     3721 atcattctgt gttctctcga aatactcctg gaatgtgagc cagcacttct catatgtacc
     3781 gccggttgcg atgacctttg cgatagactc aactactgca ctcattgcac catggtatgg
     3841 gcttacagat gagagatatg ggtcatagcc ccagcccata atggacgttg tctttgtatt
     3901 gccgtgaaga acagggagct ttgcagccat tgcctgtgta ggtgtgttct gatatttacc
     3961 gccaaatggc ataagaactg tgtttgcacc gatagttgag tcaaatctct caataagacc
     4021 cttctgtgag cagatgttga ggtttgatat catagactcc cagcggtcag cagtgtcagc
     4081 gtcgttgctc tctgctgcga aaacaggagc agggatagaa atatctgtgt gcttttcagc
     4141 tccgtttgag ttaaggaact ctcttgtaag gtcaacgata gtgttgccgc accagttcat
     4201 tttaagtctt ggctcagcag ttactcttgc caccattgtt gcctcaaggt tttcctttgc
     4261 agcctcagcc atgaactttt caaggtcgct ctttgcgata acaacagcca ttctctcctg
     4321 tgactcagag atagcaagct cagtgccgtc aagaccgtca tacttctttg taacagcgtc
     4381 aaggttgata gaaagtccgt cagcaagctc gccgatagca acggaaactc cgcctgcacc
     4441 gaagtcgtta caacgtttta tcattgaagt aacttcagga tttctgaaca aacgctgaag
     4501 ctttctttcc tcaggtgcgt tacccttctg tacctcagct ccgcagtttt caagtgagtg
     4561 gaggttgtgt gattttgaag aacctgttgc tccgccgcag ccgtcacgtc ctgttcttcc
     4621 gccaaggagg ataacgatat cctcaggctc aggtctttca cgtcttacgt tctcagcagg
     4681 agcagcacct actacagcac ctatctccat tctcttagca acatagcccg ggtgatatat
     4741 ctcgtgaacg tgaccggtag caagacctat ctggttgccg tatgaagaat aaccgttggc
     4801 agcaccaaca actatctttc tctgtggcag cttgcctgtg atagtgtcct caacaggaac
     4861 cagcggattg cctgcgcctg ttactctcat tgcctgataa acatatgaac gtcctgacag
     4921 agggtctctg atagcaccgc cgaggcaagt tgccgcaccg ccgaaaggct cgatctctgt
     4981 agggtggttg tgtgtttcgt ttttgaacat gagcagccag tcctcaggat tgccgtcaac
     5041 gtcaacagtt atcttaaccg agcaagcgtt tatctcctcg ctctcgtcaa ggtctttgag
     5101 cagaccagcc tttttaagct gctttgcgcc gatacaagca agatccataa gtgtaacagg
     5161 cttgtcagtt ctgcctgcgt aaaggtcttt tcttgtgttg atatagtctg caaaggtagc
     5221 agctatgtag tctgagtcga tagaaacgtt atcgacgata gtggagaatg tagtatgacg
     5281 gcagtggtca gaccaatatg tgtctatcat tcttatctca gtgatagtag gatttctctt
     5341 ttcaacgttc ttgaaataat cctgacagaa cttgatatcg tcaaagtcca ttgcaagacc
     5401 caagtctgtg agcataaatt tcaggtcatc gtctgttgaa tagataaagc cctcaacagt
     5461 cttgactgtg gtagggatag tatacttgat atcgagtgta tcgaagcaat caagggaagc
     5521 ttctcttgac tcaacagggt ttatcatata gtgcttgatc tggtcaagct ctgcttctgt
     5581 gagtttgcca gaaacgatat aaattctcgc tgacttgata gtaggtcttt ttcctccagt
     5641 gagaagtgag atacactgtg cgcaggaatc agctctctga tcgaactgac ctggcagata
     5701 ctcaactgca aaaacaaact cgtcagcttt tacagcaggc aggtgatcgt aagtaacgtc
     5761 aacagcaggc tcagagaaaa cgactggtgc tgcaaggtcg aaatcctctc ttgaaagacc
     5821 ctctgcgtca tagcggttta taactctcag acctgtaagg ttttcaagtc ccagagctga
     5881 ctttattccg ccgataagtg aatttgcttc aactgcaaat tcaggctttt tttctacata
     5941 aatacggtaa acagacatgg ctacatctcc ttataatagt tatctctatt atactacatt
     6001 ttttgcatac ttgtcaataa aattcttatt attcggcagg gcatataatt atgtgtgaat
     6061 aagcgttggc tcacttgttg attttttagt tcataaaagc agaaataccg caccattaca
     6121 gtgcggtatt cctaaaatat taatctgcaa acggattttc tgttggataa agcataccct
     6181 ttggctgatt gaagcctatg tctgtcatgc caagatatgt cataacactg tggagtgcct
     6241 ttccatagtg accaaaagca acagcgccat ggtgaggata gttcttctga acgagaacat
     6301 ggcggtaaaa tctgcccatt tcgttgatag cgaaaatacc gatagagccg aatgaacgtg
     6361 ttgccacagg aagaacttcg ccctcagcaa tatatccttt gagctgtgcg tcagccgttg
     6421 actggaagcg gaagaatgtt atatttccgt caacgatatc gccctcaaga gtacctcttg
     6481 aaatattagg ctcgctgtct ggctcaagat ttctcttcat gatacgctgg aacttcatct
     6541 caggctcttt tacaaggcat gaggctgtgt ttccacagtg gaagcccatg aatgtatcct
     6601 tatgtgtgta agggaacttt cctgcgatat catcattgta tatgtccgca ggaacagtgt
     6661 tgttgatgtc aaggagtgta acaggcttct gtgatacaca aacgcctatg tattcgctga
     6721 cagcaccgta gatatctact tcgcaggcaa caggaatgcc tatctttgta agtcttgaat
     6781 taacatagca aggaacgaag ccaaactgcg tctggaatgc aggccagcac ttgtttgcaa
     6841 atacaacata ttttcttgag cctttgtgag cttctgccca gtcaagaaga gtaagctcat
     6901 actgcgcaag tcttggaagt atgcctgcca ttttattgcc cttgccaagc tctgcttcca
     6961 tgtcagccac aacagcagat attctctcat cgccggcatg ggcgttgaat gactcgaaaa
     7021 ggtcaagctc tgagttttct tctatctcga tacccatgtt gtagagctgt ctgataggtg
     7081 cgttgcaggc aagaaagtcc tgcggacgtg ggccgaatga gattatcttc agatccttaa
     7141 gaccgaggat agcccttgca actggaacga actcgcttat catctttgcg cactcgtcag
     7201 gtgtaccaac agggtactca gggatataag ccttgatgtt tctcagtgca aggttatagc
     7261 ttgcgttgag cattccgcag taagcgtcgc ctctgttgtc gataagtaca tcaccgcatg
     7321 gttcctcagc ggccgcaacg aacataacag gaccgccaaa cttctttgca agaagtgttt
     7381 ctgaggactc aggtccgaag tttccaagat atacaacaag tgcgttacag cctgctgtgt
     7441 ttacctcgtc aagggctttg agcatatcat tctcgttctc cacaactgta ggacattcgt
     7501 agatatctcc gtaagccttt ttgtactcct gacagacctt gatacgtctt gattctgaaa
     7561 gcgacatagg gaagcaatct cttgaaactg caacgatgcc aagttttact tctggtatat
     7621 ttttcatgtt tcaacctccg attttcattt atttatgact ttattctagc atacctttgt
     7681 gctgttttca atagcttttt gtgcaaattt tcgtgaaatt tagttagctg cccagcatga
     7741 aaaagtgcgt tagcccaagc cactctctca cccaatatgt gaacaggtat cgtggctcgg
     7801 tgtaggctga ataagcggtg acattatcca ccccctgagc ctttgctata aggcttgcac
     7861 ggtattgatg aaagccgtct gtgacgatag tcatatcatg gctcatgccg agttcatcga
     7921 gtatcttgag agagttttgg atattttcgt aagttgaagt ggacttgtcc tccattatta
     7981 tcctgtcgct gtccatgcct ttttcaagaa ggtaattttt cattgccaag gcttcgctta
     8041 ttttctcatc atcacccttg ccacctgaaa ctatacaaag cgtatcggga ttgtcgttca
     8101 tagccgtaat tgcagcgtca agtcgtctgc gtaacattcg tgaggggcgt tcgcctttta
     8161 cctgacagcc aagcacaact ataacattgg ctttttcagg acggttttcc tgtgctttgt
     8221 acataagtat ggaaagaatg cccacatata ttatcccagc aagtatcaat gtgcttacaa
     8281 agataagccc gaatttacct gcgccatgtc cccatatttt tgatataata ccgcagaatt
     8341 ttgtccagtt tgccgttatg atcattagca tcaccgaaac gatgatgcca agcaggttgc
     8401 cagagcatat aacaggtaat ggaacaagaa acagaaacaa cagcaaagtt tccagtgcaa
     8461 gcaatattat ttttacgctc attagttatc tccttgtctt ttttgtacta ttataacata
     8521 tagctcttgt gattacaagg gcaaaagaga aaagcgctcc gcagcagcag aacgctttta
     8581 taaggaatga tctaagttga gatcagatca ccgaattttt cgagaaaatc acgcttgtcg
     8641 tcatatttag ctaccagcga tacagttttc tttgtgccaa gaccgaaaat acccataagt
     8701 gattttggat tgagtatttt tgttcctgac tgcaccacca gatcgtattt gcagcttaaa
     8761 gccgcttcat gcaaagtctt gatgtcattt agatcgttta tcctgatata agctttgatc
     8821 atgttcgtga ttatgccctt tggcatatcc tcctttcgta ataataacct ctgaaaaact
     8881 aaaaaagaac cgaaaatttc tgagattttc gtttcttttt acattggcaa cagcagttct
     8941 atacttatat gtcacatatc ttaatccata tataattata tgc
//
LOCUS       DAWEMR010000105         8940 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3247_length_8940_cov_10.694766, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000105 DAWEMR010000000
VERSION     DAWEMR010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8940)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3247_length_8940_cov_10.694766"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2581 a         2131 c         1592 g         2636 t
ORIGIN      
        1 attatcgggc atttgaatag tgtacataaa atattcatta aatattattc ttttattaat
       61 cctaaaatct cctcaggagt aaagtgatat ttcttatcac agaagtgaca ctcaacgtca
      121 gtaccaccgt ctttagaaag atcttcaagt tctgccctgc ccaaagatat aagagcattc
      181 tctactcttt cccttgaaca atcgcaacga taattaacct caaaatcatc aagaacgttc
      241 ggttcaagac cctccaaggc tttcattgca atagcttctg tggtcatacc accacatata
      301 agctttgtta cagactccat tccgtttata ttcttctcta tctgatctat agctgactca
      361 ggtgcaaacg gcaaaagctg aataagaaat ccacctgcac aattcactgt aagatctgga
      421 tttacaagta cgccaagagc gcagacgctt ggcacctgct cactgacagc aagatagtta
      481 gttatgtcct ctgctatttc gcctgaaaca ataggcactt gtccgcagta cggctcttta
      541 agtcccatgt ccttgaccac actaagcgta ccattggagc ctacagcccc tctgacatca
      601 agcttgcctt tatcgttaag cggcagttcc accactggat tttgtacata cgccttaaca
      661 tttcccatgc tgtcagaaac gcatataagc gcacctgtag gaccgtcacc gtccattttt
      721 acagtaattg aatcatcttt gcctttaagt ccaaatccca tgagcgaagt acccatagca
      781 agtctgccaa gtgccgccgt gaccacagcg gaagttttat gtatctcctc aatgcgtgaa
      841 acaatatcct ttccgtcgat agcgctgcac acaacggaag cgtctttact tataactcta
      901 actattctac ccataaaatc accctctgtt tattctcctg cacacataaa gaactctcag
      961 cgttttatca ttaactggcg agtcctcaaa tccgtcatac tttcctataa gctcaaaatc
     1021 atttaacttt acaagctcgc tcaaaaactc gtccgtatat atcctctctg tgaaatcctc
     1081 actaaaacga caataagagc cgtcctcctg ctcctcaaag aagtcaagat atatgctcac
     1141 gctgtcatca tcattgagct cattctgcca tgcacagaaa agcccatcaa gatcatagca
     1201 gaacgcattg tctgcaagga tcactcggtg cttgtacaca gtatttacat caaatatgaa
     1261 aagtccacca tcgcaggtaa attttgatac acactcaaaa gtctttctga cagcttcctc
     1321 attttcaagg tgatttatgc tgtcaagaac acaaacaaca gcatcaactg cgccatacag
     1381 atcaagctca gtcatatcct gcataagata ctgtatgttc agaccgcttt catagcgctt
     1441 atccatggct tcttccagca tttgatccga cagatcaacg cctaccacat cataatcaag
     1501 acgtgcaagt gcttcactga gcgaacctgt gccgcaggca aggtccaaaa ctacctcatg
     1561 ctctgaactg tatcgtttta taagctcatg acaccgtgcc gcaatttttc cgtaatcgac
     1621 attctctgta agcttatcat agtaacaggc aaatctgccg tatccgctca ttacttatct
     1681 tccttcttat ccaatctgtc aaaaacgata ttatatccgc ctgagccgtc ataagaaagc
     1741 gagcggttca cacggcttat agttgccgtt gaagcacctg tttcgtttac tatatcgctg
     1801 taaacatgat cctttgtaag catttttgca acctgaaaac gctgcgaaag tgctttcagc
     1861 tcaggtatag tgcaaagatc tttaaaaaat agcctgcatt cctcgacagt ttcaagacaa
     1921 agaactgctt cataaagatc gtctaaattt ttgatctcaa gtttctcttt catgaaaaaa
     1981 atctccttta cgtttcatgg tgtaaagtgt taaaccataa atatatttta acacaagtca
     2041 tgaaaaatgt aaaggagaat tatgattttt ttatttactt actcttaatt ttttcttcta
     2101 ttaggcgtta gaaaaccacc gaatgacttc ggttcatcag aaatatcctc tttatcatcg
     2161 taatattcct cttcttcgtc ctctgtatcg ctttctagtt tccctggtgg cgttatctca
     2221 gccccctcag atatatcgtc tattattgcc ctcagttcat ctacgccctc acctgtgata
     2281 gcagagaact ccacacaggt gatgtcctca aagcatggta tctctgtctt gaaagcttcc
     2341 atacgtgctt ttctggcgtt aggcttaagc ttatccgcct tggtgaacac gattataaat
     2401 ggtatctcag cgtcaataag ataatttatc atcatcagat cgtctttgct cggcggatga
     2461 cgcatatcca caagctgaaa aacaagtgca agctgtctgc tgccgtcgtc aagatagcct
     2521 cctataagac tgccccactg ccttttctcg gactttgata cgctggcata gccatatcca
     2581 ggcagatcaa cgatatagat attctccaca gaaaagaagt ttattgtaac agttttacca
     2641 ggcatagagc ttactctcgc caagtttttt ctgttaagaa gcttgtttat cagcgacgac
     2701 tttcccacat tcgacctgcc ggaaaaggca aattcaagtc tgtcagactt gggtatctgc
     2761 gagaattttc caaaggaagt ataaaattct gctttgttat aattcatagg cggccctttc
     2821 tataccgtta atttttttcg ctccggctcg ttcttgctaa ggagttcaag cggaacatta
     2881 tttttgctgt ttggaataag tgcaacgtca agcacatcct gaatagtctt tgcgaacacg
     2941 aactcacaac aaagctttac tgtgtcatca acgtcatcaa gatcgccctt attggcaaaa
     3001 ggcacaataa tagtcttgac gcccgccttg taagccgcca tagtcttttc acggagaccg
     3061 cctataggca gtactttgcc gctaagagtt atctcacctg tcatagcaac gtcactgcgt
     3121 acttttatat tgcccaatgc agagatgatg cccgttatca tggtgacacc tgcagatgga
     3181 ccgtccttag gcacagcacc ctcgggagcg tgtatatgca aatctttttt ctcgtaaaag
     3241 tctttatcga tgccatattt gtcagcaacg cttctgcaat agcttacggc aatctttgca
     3301 gactccttca taacgtcgcc aagtgagcct gtaagctcta ttctgccctt tccgtcaagt
     3361 acaaggactt ccaaaggcat gacaactccg cccactgacg tccatgccag accatttaca
     3421 gagccaacag catctttatc tgatacaaca tcagggagat atttctcatg accaagatat
     3481 ttttcaagat tatccgcctt gaaagtaact ttcttaacgc cgtcttcaac tatctcctta
     3541 gcagcctttc ggcaaagcga gcctatctgt ctttcaagat ttctcacgcc agcctctctt
     3601 gtgtagctgt ctataagctt atagatagca tcattgcaga tgcgtatctg agatgcttta
     3661 agaccgttct taacttcctg ctttttaata atatgttcct ttgcaatatg gaacttctcc
     3721 tccctagtat agctagacaa ctctatgact tccatacggt cgagaagagg tgcttgaaca
     3781 gtatcgagag tatttgcagt tgtaataaac agcacatcac ttagatcaaa cggcacttca
     3841 atatagtgat ctctgaaagc attgttctgc tcgctgtcaa gaacctcaag catagcagac
     3901 gatggatcgc ctctgaaatc attgctcatc ttgtctatct catcaagaag aacaacagga
     3961 tttttacttc ctgcaagctt catggcattc attattcttc ctggcattgc accaacatac
     4021 gtctttctgt gacctctgat atcactttcg tccttaacac cgcccaatga gacccttaca
     4081 aagtttcgat taagtgctct agcgacagac tttgccactg aagttttgcc aacgcctgga
     4141 ggaccaacaa gacagaggat ctgccccttg acatcaggag taagcgctct tactgcaaga
     4201 ttttcaagta ttctatcctt gacctttgcc atgccatagt gatccttgtc aagtatctgc
     4261 tttgcctttt taacgtctgt cctatccttt gtgacagcat tccacggcaa tgcaagcact
     4321 gtgtcgagat aatttctcag cacaaccccc tcctgtgaca tcatacccat cctggaaagc
     4381 ttcctgacct cgcccataag cttatcacga acttcttcgg acaatacaag tgaatctatc
     4441 ctatgtgtgt actcatcgat ctcatttaca tctgagccgt cttcattttc gcccaactca
     4501 ttctgtaacg ccttgatctg ctcacgaata tagtattcgc gctgactttc ttctatctga
     4561 gaatggacct gactatgtat ccttctttca agagataaaa cattggtctc tcgtgaaagc
     4621 atagtgatca taagaattag cttatctaaa acagatgggg cttcaagaag cgactgtctg
     4681 tcagtaaaag ctaacggaat attaaatgcc aaactttcaa aaagttgaac aggcgtttta
     4741 gaaccgagta tctggacata aagctcttca ggcattttag gaaccacctt aacatactct
     4801 tcaaactcat ctaagaccga acgaaaaaca gcaagttttt cgtcatcgtc aagcctatcc
     4861 tttgaataat ttggtacaag tcttacatca gcttcatagc aatcatcata ctgataaaac
     4921 tcagcaagtc ttgctttacg cagaccttct acatagcatc tgtatattcc atcaggtgct
     4981 ttaacgatct gtcttacctt tgccacagta cccaccttga acatatcctt tttttcaggc
     5041 atttcaagat aaacgtcttt ttgcgcacaa agaaaaacaa atcgatcgcc gtccatggca
     5101 tttttcatgc tctttatagt tcttggtctg ccaacatcaa aatgcataga cattccagga
     5161 aaaactacca gatctctcgt tggaacacac ggcataatat tatttatttt aacatcagtt
     5221 ttatctttca tagttgctcc atttccttac agctctacaa atactgtaat gctttgttat
     5281 acagctttag tatataatat cacatttatg atacatttgc aagccccact cagaaacttc
     5341 cccttaacaa aaagaggcac ctataaaggt acctcaaaat agaaaacact attagtcagc
     5401 tacatatgga agaagtgcca catgacgtgc tctcttgata gctgttgtaa gttctctctg
     5461 atgataagca caagtgccag ttacacgtct agggaggatc ttagctctct ctgtcatgca
     5521 ctttctaagc tttgcgatat ccttatagtc gatcttttca gctctgtctg cacagaacat
     5581 acaaaccttc ttacgacctc tcttcatagg tctgcttgta tttctttcct tttccatggt
     5641 aaatttccct ccttagagaa atattttttc aaaactcctc tgcttagaat ggtactccgt
     5701 catcggtcaa tacttcttca aaatcatcaa ggtcaccaat ggacaaagcg tcatttgatg
     5761 gagcattgtt atagttattc tgattagaat tgtttgcagg ctgtgaatat ccaccattgt
     5821 tctgctggta attattctgc tgataaccac catttccgta gttattggaa tagttacctg
     5881 ctccctgctg ttttggttca cctgtgaaag acacactgtc aacattgacc tcagttgtat
     5941 aatgctttac accattcttg tcatcataag aacctgttct tatctgacct tcaagagcga
     6001 tcattctgcc ctttgaaaag tatctgttga taaattcagc tctctgtccc caagcagtgc
     6061 aagtaataaa atcagtctgc ctttcagcac cctgacgaac tggtctgtca actgcaacat
     6121 tgaatctcaa aactgcattt ccactaggag tctgtcttac ttcaagatcc tgagtgatcc
     6181 tacccattaa aataactctg ttaagcataa cttttccctc tttcgcagtt ttacttgacc
     6241 gttactaaga aacggatcac gccgtctgtg ataccaagaa ttctctcaag ctcagctgga
     6301 aactcaggcg tagatgtgaa tgaccacagt acataagtac cttctgtttc ataattgata
     6361 gcataagcca gcttacgctt gccccagtta ctaagtgtaa cgtcgcttgc gtttgcctcg
     6421 atcaagtcgt tgaacttagc tgtgagtgcg tttacagcct cctcgtcaag cttagagctg
     6481 aaaattacca tagcctcata agactcatta atttttgcca ttgcatttgc acctcctttt
     6541 ggatttcgcc tgtgccgcaa tttgcacaag caaggataac tcaattatta taacacaaga
     6601 ccgattatct gtcaagtgtc acttgcgttt ttttatgaac tttttgtaaa atcatgcaaa
     6661 tatctttgta acaccaaaaa tgccaaacac aactatcgca gaataaacgg caaagccaag
     6721 aataacagcc ccccatgacg taccgccctt tgctactatc ttttcggcta tctcctgctc
     6781 agtgccgtcc tccgacctta tctcacggat ctttttttgt gcaaacttat aatacatata
     6841 attgccgaaa aagcctgcca caaactgaag tgccattgaa acataccatg tcgcagcgga
     6901 aatgccctga aaaacccttc cggtcacatc gatgccgata tcaagcgaga aacccattgt
     6961 tcctgcacta agatattcta tcgcagtagg gatattgagc agtgctgtag ccacaagcat
     7021 gatgataccc acaagattca tttttctaaa cagaaaatat aggtgtggaa aaagaaaagc
     7081 cgcaaagttt atggaaatct ttccaccaaa tttaccaaaa cgtataaagt tagacaagaa
     7141 tatgagcgga tttgtcttta caaattttgc atagtcatct agtttaatgc cttcaagatc
     7201 tgagtcctta tcaaatacaa cctgtctgcc aaacggaccg taaggctgtc cgtcagggcc
     7261 ggtctgctga gcgttatttt gcgtgtccat gtcgttgaat ggacgttcct cagtatcatt
     7321 tttcgtaagc ggtgttccgc acttttcaca aaacagcttg tcaggcggat tttcaaaacc
     7381 gcagcgtata cagcgtatat tctcactttc tacactctcc tgagtttgct cccagctctg
     7441 acaactttca tgcaaagcgt catttatgca ctttccttct ttgagataac actcacgatg
     7501 ataaggtgtg ccacactcag gacatacaac tatatcgtca ccatctttga atacttctcc
     7561 gcaggaaaag catttttttc ctgtataatt agccatatcg ggtttccttt cttgctgaaa
     7621 caggcttttt aaagccttta aataatatta tagcacaact ttcaggaaaa tgctatagtt
     7681 tttttatatt tttcctttac ttttcacaaa aaaagtttta taatatataa tgatataaaa
     7741 taaggagaaa aagatatggt atttttagaa aacttaaagt cagagcttga agctcttaga
     7801 cctatcatca gcgagctgca tgacgtatac gatattgaac gttctcagga aaggattgca
     7861 gagcttcacg aaaaagccgc agagcctaat ttttgggacg acatggagaa atcacagaag
     7921 gttttgcagg aaacaagaaa tcttgagggc aagctggaaa aatacaataa attcaagtcg
     7981 tcatttgagg atatcgaagt gcttattgaa atggcagccg atgagggtga cgaggacatg
     8041 atcgacgaaa tacagtctga gcttaaaact ctaaaagagg gtcttgacgc cgcacagctt
     8101 gacactctgc tcacaggcga gtatgatcac aacaacgcta tcatcaactt tcatgcagga
     8161 gcaggcggaa ctgaggcgca ggattggagc gaaatgcttt tcaggatgta tcacaaatgg
     8221 tgtgaaagtc acggcttcaa aatgactact cttgattatc tcgagggtgc agaggctggc
     8281 atgaagagtg cctccgttat cgtggagggt gaaaacgctt atggctatct taaaagcgaa
     8341 gcaggaatac acagacttgt gcgtatctca cctttcgaca gctcaggaag cagacacacg
     8401 tcattctcag cggtggacgt tatgcctgaa atagacgata caatcgaagt agatatccgt
     8461 cctgaggaca ttaaaatgga cgtttatcgt gcaagcggtg caggcggaca gcacatcaac
     8521 aaaacgtcat cagccgtaag acttacccac ataccaacag gcatcgttac atcatgccag
     8581 acacagcgtt cacagttcca gaacagggat tatgcaatga aaatgctcaa agcagagctt
     8641 atcaaaataa aagagcgtga acatctagag aagatagagg atatcaaggg tgttcagaaa
     8701 gagatcgcat ggggtgctca gatacgttcc tatgttttca tgccatacac tcttgtaaaa
     8761 gatcacagaa cgggttttga aaacagcaat gtaaacgctg ttatggacgg cgaccttgac
     8821 ggtttcataa acgcttatct caaagcgctt tcacttggcg agatcgaaaa ataattagca
     8881 taaacaaagg cgctgtaaaa aaaagcgcaa caaagcccgt ggatttcctc gatatggaag
//
LOCUS       DAWEMR010000106         8934 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3252_length_8934_cov_11.187972, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000106 DAWEMR010000000
VERSION     DAWEMR010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3252_length_8934_cov_11.187972"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2371 a         2184 c         1669 g         2710 t
ORIGIN      
        1 tacccaacat catacgaaca gtaaacattg gctaatcctt gatgaatcag ttccgtatca
       61 tcataaacct caacctctgc tttaatctcg ataccatcag ttactttaca ataattcata
      121 aagtctttaa tagcatttat actttcgtgt ttatccatag tattaccccc cttcatacca
      181 ataagttccc ctcaaaatca tatatttgag tatctgtgct ccaacattta tcacaataca
      241 tagtaactat gactttatca tcaacttcgc tccaactttt tgcaggctta tacgccgaag
      301 aaaaaactgc aaacttttca cttgcatcag ttcttctgta aacataaaac atcttcttag
      361 ccccacattt cgggcataca tattctcttt caaaaaaata cataatgact cctcctatta
      421 aatcagcggg tgcttttcaa agccccatga agtctcaaag tcattccaat cctgtttgca
      481 aatttcaaca ttctctttaa ctatctcatc tattttaggc tcaatctcgc catttacaat
      541 aattgggaga ttttttacat ctctaacttg ggtactaagt gttgggttaa gaattgcaaa
      601 gtataacgga gcaattttcg ttccaaatat tccaagacaa taccatagct tttttacgct
      661 aacaatactt ggaccttttt tgtcaaaaat acaaacaggc ggcagatacc taaatgtatt
      721 tacactactt gtcagctctg tataagtaat tccctctttg aacctatact tttcagccag
      781 aagatttgat gtagagttag ttttatagta ttgaacagca ccttccgacc agtcaacaac
      841 taactcaata ttaccgtacc attttctgaa atctccaccc tttgcatagt aaacccattt
      901 tttacccatt ccaaccttgt tagcatctat ttcccaatga agcctcaaat atttatcatt
      961 gttcgccgtt atatgctgtg accctgtaaa gtcgctaata ctgtcaatac tgataccacg
     1021 ttcaaacgcc ctaataaaat tctcactcac ccaataagcc acaggcatac ccggaatctt
     1081 ggagaagttt tcttgtttgg ctgcaaaacg cttatcaccg ctcaaaaaca ggtcacgctt
     1141 ttcattctcg cccacagtgt caacaagcct tgcgtatgta cccttgtagt ccttgatatg
     1201 ccccgtcata acaaacgcag ttgtctgcac gacctcgccg cctatctcat caaaagccct
     1261 tgcaccgagg tgcgccatgt tgaccgttgt tttcagcatc agcttacggc gtagattttc
     1321 atagcttgac agaaacatga aagcgtgctg tgtaatcatc gcatagaaac cgtgcggctt
     1381 tgcaagctgt ccgcacttct cgataaagca ggcgaacaga tcggatttac tgtcaggaaa
     1441 cttactcttg acaaactccg acagcttcac attcattccc gaaccgccca tatatggcgg
     1501 atttgtgcag actacatcat acttctgtga aagtatcttg tatatcttca tcatcatgtc
     1561 aaactgtcgc atattcagcc cgaaggtggg cataaagtcg gaaaaagcgg tgtcgatatc
     1621 agcaggggcg gtgacggtca tcagcgagcc gtaggtatcg gcaaaggcga actgctccgc
     1681 aaaacgcctg atactgcccg agagcatatc cgtgtcagca cccataacat cggcaaaatt
     1741 cgcaagattc ggctgatttt cgggactgca aatgcggcgg ttgtactgcc ttgctttcat
     1801 catcaccgca aaatacgcaa gctgataggc tctgcggtcg atgtccagcc cgtagagatt
     1861 gtttcgcagg atagacttcg ccgcgtcacg ctcaccccag ccgcaggaac ggtaaatgtc
     1921 gataagcaca tcgaaggcat acaccagtat atgtcccgaa cccatacatg ggtcgattat
     1981 cttgatattt tctggttgaa tatttttata ttccgccctg atcttttcta gctcagcctt
     2041 gacgggatcc tcctgttcgg cttcatcaag ataatatttc cagtcggcat catcgggctt
     2101 tccgtgaccc tcagcccaaa gccgtccaag ggaattttcc accatataac gcactatcca
     2161 gtcgggcgta aaaagctggg tagccgcagg gatagcctgt gcagagatct tgatattctt
     2221 ctttagatca gcgaaaacct tgtctttcgg ctcggtgttg taatactgat acagccagcc
     2281 gattatctgc acctgatctc tccagtcctc ctcgggtatc tcggttatca tcttagcaat
     2341 aacgctgtcc tcacgcagca gatttcttgg gaaaagcaat tccgtccagc cgcctatctg
     2401 ctcaaacatc tcgggcaaac ccttgttcaa agcattgcac tgcgtgatga taatatattt
     2461 atataaccgt tcgttctcct gattttcgag cagttcaagc acaagctcct tgtcgagccc
     2521 gtcaatctca acattagctg cgtcggaaag cacctcgggc ttgaaatttc cgctctcgtc
     2581 cgagaatatt ctcgtatggg acggcagata attattgacc tccatgaatc gcagtgcgat
     2641 gaaacgattg aaccaagtat aggcggcttc ctccatcgtc tgcgtaaagc cgttctgacg
     2701 gatatttgcg ataagctgtg aacgctgatt tttctccgca gccgtaagcg accgtccgtt
     2761 gacagactcc tgcgcagggt catttgtgcc gttctccgtc acttcaaatt cataagcaag
     2821 ctgtgttaca gcattgataa gcattactct cgcttcaaca gcaaaatttt tgatagcagt
     2881 tttgttcata ttttcatatc tcctcagatt atatgcagaa catcgtgacc gttcagcttg
     2941 tccatgagcg tccttttgac ctccgcaata tactcgtcaa tatcggcttc actttgcagt
     3001 ttcatcgtgt ggcaaactgc actgcggctc attgtcacgg tatcaatatc aggttcgtca
     3061 acgacaacga gtttttgcag atattgctgt ctgatattgc cgatctgaat tttcatcgca
     3121 tcgagattta tgagtttgtc tgcattttca gttgcggttc gcttttctgt cagcgcatcg
     3181 tccgccctct gcacgatgtc accctgcttt atgtctgcgg tctggtgtat ctcgcccata
     3241 gccgcctgta tctcgccgat gacctcctca cgcttttgcg acaacagctt ttgataatcc
     3301 tcctgcaccg tttgtataag ttcgggcagt tcgctgatac gcttgtaagg cttctgcgtg
     3361 gcgattatct gctttatctt ttcgacaacc gcaagagtat tgtcctctgt ctggaaatac
     3421 tctttttcct cattcacacg ctccacctgc gaccttgcat tatcgaaaat gcttctctga
     3481 acacggaaaa acgtatccac ctcagacata tcctccgcaa gttcaaggaa atcttcctgc
     3541 atatccacag ccttttttag cagagcgata ttgtcatttt tctgccccaa aagctcattg
     3601 cacagcttga taccgttctc cacaacaccc ctgccaggat aagcatttgc actatattct
     3661 tttataatca agtcattcag cttgttacgc tcagtttcaa atttttctat gatataagca
     3721 ataattccgt cctcatcatc gggtatcgcg cccaaagtgc agttgaaata ttcgctcaaa
     3781 aactccctcg cctttttgat aagtgcagtc ggaggagcga ttctgaaccc gatgatcgcc
     3841 ttgtctattt ccgttctctt gcgaagataa tcgggcattt ttttatcact cggctgaatg
     3901 acagcaccgc cgtatttgag ggtcaacttt tgacttgcga taagctcggc agtcacccct
     3961 gctatgtcga tctctcgcca gccgtaggga atagcctgat aacgtttctg aatatccccc
     4021 atagaggtcg gcaactgttt cattttctga atgtcaagat attgatataa ttctttgaca
     4081 gcctcggcat ttggtgactc cgcaccatca agcatcatct gcttatcacc gctgagtatc
     4141 tgcaaaatat ctgcatcgct gtcataattc tttcggacgt aactcagctt agtataaacg
     4201 ctctccacga gaatcgacat tgctctttca atacgttcct tcaccgatga aaccttcagt
     4261 gttaatttat cgcctgcaac gtaaactctg ccctcagcga tagccgccga aataagctcc
     4321 tccgcacgtt tttcatgtgc cgatgcctga ttctgcctgc ccctgataat actctgtatc
     4381 gtctcgggga gctgtgccac gttcttgctt ttgacatagc ggcgaatctt catggcgtct
     4441 tccagttctt taaaatatgg ctgactttcc gcaagcacaa cgataacttc attgtccgcc
     4501 gctgatctca tgagaaatac agaatcatct gttgtgtata tctccgatgc tactgtaata
     4561 aaatgtaatg ccaccgagcc tgtcaactgt ccgataacgg tttcgtcgat acgctgatcg
     4621 taaggaaaat catatttgcc gtacttgaat ttcttgctga caaacagctt tccaaagata
     4681 atatctgaaa ttgccttggt gattatcgca ctgtcaacag gggtgttctt tatctcacgg
     4741 gcaatatcct gttcatcatc ggtcaggaat gtatatgtat ctcctgcgcg ggaaacatag
     4801 ttttgagaaa caagcctgtc aagggactgc tgtatcgaaa gccttgtgga aaccttgtcg
     4861 gttcgtatat cgtccgccat aagaatgctt atattgtcaa tattcgcctt gacatcatcg
     4921 gcatatctca caaggtacag cagctttaat atatttacat ctgtctgttc aataccatca
     4981 cgattgtcgg cagcattctg acatcggtcg ataacacggc ggatagagct ttctaggaaa
     5041 gtatgcactg tgtcatagaa aagatagaac ggcacaagag catactcgtc cctgtcctga
     5101 tttgcctgtg ccgcctcctg aaatcccgac agcatggaac gctcaccacc ggaaaggtgc
     5161 ttgcccgaat tgccgtgctt gcgtatttcg gcaaggacgt tttgcatcag cctaaactga
     5221 tacggcacaa agggataagt ctcgataaac tcgccctcgc ccgaataacc tttcaggtca
     5281 agaacggcat tattgaacgt gaacagattt ttcagcacct gattattttt ctcatattgc
     5341 aggctaagca cctgttctgc tgtatcggtc ttggcaagga tacgcttttt gatgacttca
     5401 tcaacgcttg aggagctaag gctcagacgg gtattgaatc tgccctgaat ctttgaaaaa
     5461 tcatcaccgc ttatcttggt aatgctgtcg atagcctcct gcgaagtcac catgacccac
     5521 actctgccgc cgcatttcgt gcctatctct tcaacgatag tctgtaagtt cagcatgagg
     5581 tcagaatccg agccgatgta ctgaccaact tcatctatca tgaacataag gcggtagtcc
     5641 ctgcccttgc tatcgatata ctcacgaatt tccttcacga gctgttcgat actaagctca
     5701 atttcttcct cgccgttgaa ccagtttcgt gcagcagttt cactcatatc gagggcttcg
     5761 gacatagctt ctacaatatc gtcctcaaag aaagcaaagg aatcacggct ttcttcccaa
     5821 gtaccaccgt tgatacgctc aaagcattct ctgaactgct ccgttttacc tgactttgcg
     5881 atgaactgct ccagttttgc gactttcaga tcgttgccgt aaaagccctg atgttcatag
     5941 aatacttttg cgaaaactct taaaatagcc gttttatctt ttgtcagagg gcttttggag
     6001 tctatattga aaagaatagt ttcggtagga acttttacac agttttcaag ctgtgcgaac
     6061 atcatggggt catcgaactt atccgagaaa tactgcactg ccttttttcc accaaccgtc
     6121 ttattcgcaa gcagatagga cagcattttc agaaagtgag atttaccgct tccgaaaaag
     6181 cccgagatcc acacacctat cttgtcggtg ggctgttcca acgaacgttc ataatgattc
     6241 agaaatgtat tgaaatgacg gcgcagctcc ctcgtgataa tatactccga aagttcctgc
     6301 acgatagact gttcatcatc ctgtgcgacc ttgataacac cgttaatgtc acggttgatg
     6361 tcggatttat agatactctg taaacgcata aaaaaactcc ttagatcata ttgaaagcac
     6421 ggtagtagtt gccgtcctca aattctgcaa acagtgaaag ttcctgacca ttgtaagcac
     6481 ctggataaag catcaccacg ggaatatccg agaagatatg ttggatattg tcaaggatat
     6541 tgtgagaccg catgaacgga taaacttttc cgactccggt aatcaacaaa atatccccat
     6601 gctcatgcgg ctcgtatttc attttttcta caaaggcttc gggcgaaaca gctttttgca
     6661 actgtttaag cagacactcc gagccttttt tcttctccat attgggaatg cctttcagga
     6721 tccgcttttc ctcgcatatc cgcaggaaaa tcttatacag atcacactcc acgatacgaa
     6781 atggctgtcc ctgtgcattt atcaggtcag caaaatagct ccgcacaagc atttcgtcct
     6841 ttggttcgta gcagaaaacg tgtatcccga cttcatttga aagccccttg ttgctcagaa
     6901 acacttcatc tgaaaagcgt ttttttatct cattaagctg gtaaataata ttactcatag
     6961 tccgtacctc aattaaaaca attaaaagct gcaagtgcat ctgtgtcgcc tttggaacgt
     7021 atcaattcat caagctcggg atacaaatat acaggaagca aggtctgcga cttcgagctt
     7081 tcaagatatt cgcactctac aagaaaacgc accaacacct gcttaagctt tttcattgtg
     7141 gattcgctcc attcactgac agcaggcact ttttcggcaa gggacataaa gaacacattc
     7201 acatctttca ccgaaaaatc aagctgctgt gagcgatatt tctcaccgat tacctcgatc
     7261 ataaagtcat agacgatact gttttcgcac atgatcgcat acaggttcac ctgctttgca
     7321 acttcggcag atgcatctgc aagcagcctg atggcaactt cattatcaac tgcgtctatg
     7381 cgcttgaaac aggtattggt gatcgttttt atcattcgct ccgtaggaaa ctggaacaga
     7441 ttttcgctct gtatctcacg atatatctca tcacggcttc ttccccgacc aagtagccct
     7501 gcgactattt ttatctcata aaacaggaac tgctcacggg tgagtccgcc gctgtaatgg
     7561 tttttgtgca tttattgtct cctccctata tatagcctga atcgcaatta ggattctgag
     7621 atttttctca aaatatatgc attcgagatt atcagcaaat agacatgttc tttcttcatt
     7681 attcagtttt caagatgcag ttttgacaca tttttacact aaaacagcat agcaagttta
     7741 ttcatgagct ttctttcatg aacataatca gggtggacat acctgtttag tgtgatattt
     7801 accgttttat ggccgagaat catagaaagt gttttcacat caaacccgtt ctgaatgcaa
     7861 tttgttgcga aggtatgtcg aagcccatgg tatgtgtgat caccggaatt tgattccgac
     7921 agaacccttt tataccgata ctggtaggca cgaggttcaa ctatcttttc acttcccgaa
     7981 agaacgtagt agttatcatc gcctttaaat gccatgaaat attctgctag gaactccggt
     8041 attgcgatct ctctgaatga tgtatcgctt ttggggacgg agataataat tttcgttttt
     8101 ttaccgtccg aattgcaggt gcagattcgc tgaactgtac ggtgtacata taatacccga
     8161 ttctctatgt ctatatcagc ccatgtcaga ccgcataatt cgccgattct cagacccata
     8221 taaagtgaga tcaaaatccc cagacctgta agggtcatat gttccttaat gtgtgagata
     8281 aggactttct ggtcattatc actgaatttt tctactttct tcttttctat tttgggaagt
     8341 atgacatttt tcagagaaag gctgaaacca tactcttcct gtgcatatat aagcatcgac
     8401 ttaaaaacgg ttataatatc ttttatataa cttgcggaga acccctctga aagttttttg
     8461 tttatgaaat tgttgagctt aactgcgttt atatccacac agggaatgtc gccaaattca
     8521 ggaataatat gcttttcaaa cttgttgcag taattgacaa atgaggattc tttgacacgg
     8581 ttggcgattg cagtgatcca ttcccgatat acttccaaaa cgctgataca ctttccactc
     8641 tgaaatctta attctacttc cattccaatt tgcacctttt tataagcatc atcataggtg
     8701 tttccgtaaa catattgata aacagctttg ccggattcat ctctgtcgat gataaagcgt
     8761 cccatgaatc ttccgtcttt tcttttggta atacaaatat ccaacttact cgccttcact
     8821 ttctattgtt gttatcacac aatatttatt gttcaaacta tacagaatta acaaaattaa
     8881 caaatgcaca aaaatacatt gaaaaaaatc gccgattatg gtataatatg aata
//
LOCUS       DAWEMR010000107         8918 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3257_length_8918_cov_10.376735, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000107 DAWEMR010000000
VERSION     DAWEMR010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3257_length_8918_cov_10.376735"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    931..940
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3415..3424
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2906 a         1754 c         1867 g         2371 t
ORIGIN      
        1 tgtgctgact aatgacaacg aatatacgac atataattgc agatacgcca tttttgcaac
       61 tggtggaata ggcagagttt acgaatacac tacaaactcc gctatcgcaa caggcgacgg
      121 cataactatt gctcatgagc ttggagcaga gataaaaaat ctcagctata tccagttcca
      181 cccaacaggc tttaacaaca agcacactcg tgaaactttc cttatatctg aatctgtcag
      241 aggcgaggga gcttatctta aaaactgcaa cggcgaaaga tttatgcaaa attatgacga
      301 ccgccttgag cttgcaccta gagatgttgt ttctcatgcg atcatggctg aagccaagaa
      361 aacaggctct gacaaattct atcttgatat cactcacaaa gaccctgagt ttgtcagaaa
      421 cagatttcct atgataaatg aaaagcttct cgaagcaggt tatgacatga ccaaggatat
      481 gatacctatt ttcccttgtc atcattacct tatgggcggc ataaatgttg atacatatgc
      541 aagaactaac attgacaggc tctatgcttg cggcgaatgt tcacatacag gtgttcacgg
      601 caataacaga cttgcaagca attccctact tgaagctttg gtattttcaa gacgtgctgc
      661 ggctgacatc aatgagaaga taaaatctga cagtggtgac cttgaaactt acgatttcaa
      721 gatagacaat gaagcccctc acattcctca gggcataaga acagagataa gagatataat
      781 gcagcgtgca tatttcgtta tccctgataa ggaggctttg ccacctgatc ttgaagaagt
      841 aacaaagcta aaaaatttcc tttgtgacgg taatttcaaa atagacagag attatgttga
      901 agcaaggtct cttacaactg tcgcttatat nnnnnnnnnn attaaaggac gctatcgaaa
      961 agctcaaatc caaagaaaaa atggaggaaa acaaatgaga ttaatgcagt ttcagatcga
     1021 cgatatcatt aaaacagctc tgctggagga tatcaactat atagacgtta caacggatta
     1081 tcttgttgac gaaaattcag tttctacagc aaagtatgtt tcaaaggacg aaggcgttct
     1141 ttgtggaatt gatgttgcaa tgagagtttt tcagcttctt gacagcaatg ttaaatgtga
     1201 gatatttatc catgacggtg aaaaggtcaa gaaaggcgac attatcgcaa aaataacagg
     1261 ctcaacaaga gccttgctca aaggcgaaag aacggcactg aatatcgttc agcatatgtc
     1321 aggcgttgca actgcaacaa acagatgcgt tgagcttgta aagggtacaa aggcatctgt
     1381 tgcggataca agaaaaacgc tcccaggtct tagagttttg cagaaatatg cagttacagt
     1441 aggcggtgga aaaaaccaca gatacaatct ttcagattgc gctatgctaa aagacactca
     1501 ccttgacgca tatggcagca tgactggtgc ggtaaacgct ctcagagaaa aaatggggca
     1561 tactgtcaag atagaagttg aagtcggaaa ccttgaagaa ctcaaagaag ctcttacact
     1621 tggcgttgag ataatcatgc ttgacaatat gacaaatgaa gatatggcaa aggctgttga
     1681 aatgacaaat ggcaaagcac ttttagaagc ttcaggaaac gtgacctctg aaacaatccg
     1741 tgcaatagct gaaacgggcg ttgatatcat ttctatcggt gctatcacac actcagtcaa
     1801 ggcgtttgac atttcaatga aaatggaaaa gtaagataaa acaaaagcca acgccatatt
     1861 aaggtgttgg cttttatgct taaatggggg aaaaatatgc gttattacaa aagatctcat
     1921 gtgcattaca gcaaatcagc aatgaaaata atagcgttag ttttcatgtt ggttggcgtg
     1981 acatttcttg cagttggaat aggttttgca atacaccaaa ctcaactcaa aaacagatgt
     2041 actcttgaag tcaatgctat aatatcagac attctcagta aagatgaacg acatgagaac
     2101 gatgacggac acgtatcata tagcacagtt tacacaccag tgtattcata taacgttgac
     2161 ggacagctct acaccactca cagcaataca tattccagca acatcagata caaaagagga
     2221 caaacgatcc agatattctg tgaccctgat gaccctgaaa cgttttatgc accaaatgat
     2281 acaaccaata ccatattaac agttgttttc tgtggattag gcgggctgtt tgcgactata
     2341 gcaatagtgc ttatcatagt gcttatacat ataaagaaaa agcagaaatc cggaaaaaat
     2401 ttttcagaaa atgaattata cgaagaaaat aattatccat acgacgaata attctgagag
     2461 gaggaatttt tatggcaagc attatggacg gtgaaagaca atttcacatc agaactatcc
     2521 ctgatgaagt gggcagatat gttattatgc cgggcgaccc gggcagagtt cctaaaatcg
     2581 ctgctctgct cgataatgca aagcaggtgg ctcaaaacag agaatacaat gtttatacag
     2641 gctatcttga cggcgaaaag gtaactgtgt gctcgaccgg tattggtgga ccatctgcgg
     2701 caattgcagt ggaggaactg gtaaagtgcg gcgcagacac atttattcgt atcggtacaa
     2761 gcggaggaat ggatctttcc gttagcggtg gcgaccttgt tatagcttcg gcggctgttc
     2821 gtggcgaagg tacgtcaaaa gaatatctcc ccgagggcta ccctgctgta gcagatttca
     2881 ctgtaacaac tgcccttgca aaagctgcgg ctgaactgtc agaagataag ctcggcaagc
     2941 gctatcatgt gggcgttgtc cacagcaagg acagctttta tggagaagta gagcctgact
     3001 caatgcctgt gagcggcgat ataaattacc gctgggacgc atatttaagg tgcggctgta
     3061 tgacttccga aatggaatgt gccgcagttt tctcagtagg tatcacaaga cacgtccgct
     3121 gtggagctgt tataacagct atatggaacg tggagcgttc aaaggcagac cttgaagaca
     3181 atgtgagcga cgacaccaca agaggtatcc actgtgcggt gaacgctgtg aaaatactga
     3241 tagaacagga taaaaagtga taaaaagccg ctgtgacgtt tattgtcata gcggctaaaa
     3301 ttattgtatt cttattccaa ggagcattgc aagctccaca gcttgattgg ctgaaaaaac
     3361 agcgtttcgg agttctctga atgtatcaga aacgcgaagt ccacttacaa gacannnnnn
     3421 nnnnatttac ttccgcttgt cttaaacgct tttaaattgc aggaaacaag ctcagccgag
     3481 gacatatcac tgtttttcac ctgggtttca gacagcttgc acttatcaaa aatagaatat
     3541 ccgcagtttg aggattcaaa ggctacactt tgaatggagc aatccttgaa ctctgcgcca
     3601 acgagcttgc aggagcggaa ctcacatctg ctgaggtagg atgagtccag tgatgaattt
     3661 gacagatcgc aagatatgaa cgtcacatca acaaatgagg aacgctcaaa atcgcaatcg
     3721 gtaaaggtgc agttttcaaa catacagttt ataaatgaca ggcaggagag atcttcgctt
     3781 tcaagatatt ctccgctaaa tttacagcct gaaatatcac tgtcagtgcg ttttgcagta
     3841 cgtttgactt cgttaaggtc gcacaatgga aggtcttgca tcttattcac agctttcctc
     3901 ccaattgtca gaaaaatcaa aatcatcagg aaactgctct gctataactg tctttgaaaa
     3961 ctgtgaagca gtttctataa gctctttgcc tcttcccgtc catgtgagca agtctgcatc
     4021 tatatcaaca gcctgaaatg cagtttcaag gcttatagat ctgaggtcgg cagcaatgaa
     4081 ctgaggcttt gaaataattc tccagataaa cggctgagca caaatgaacc tttcgatata
     4141 ctcaaggtct atattttttc tgtattttga gacaagctga ccccttgtga cttttggtga
     4201 atgaaaccta aaccacatca ctgagaatgg gttaaagctc tgcactgcaa attcgccttt
     4261 gtatttcttc atcatatggt caactcggct ttcaagctct ccaaatggcg caccgccttt
     4321 aagctcaata agaagtggca ctttgccgtc aacagttttc agcacctctg acagcagtgg
     4381 tatcttttca tcactgtttt taagtttaaa ggcagaaagc tgttcatatg tgtaatcaaa
     4441 caccttgccc tcaatgccgc acattctcat aaggtcattg tcatggaaaa ccacaatgcg
     4501 gcaatccttt gttagacgta catcaagctc gatagaaaga catttttcaa ccgccagcct
     4561 gaatgcggta agagaatttt caggaacaag ttctgagtga agacctctgt gagcaatata
     4621 tttgccaaaa tccatatggg atacctcttt tcgtattaaa gtcatttata ttatactctc
     4681 ccaggcttag attgtcaact gtatcacgct ggatcatata tatttcattc acagaaagtt
     4741 taagaattat cacacagtat catcacaata ataagatata attaaattta tgttatgcag
     4801 aggattttct ctcaaaggaa aggacaatta ttatgataaa gaaattagcc gctgttacag
     4861 cagctctcac aatagctatt tgcagcttta cttcatgcag caaaaataac gacagttcat
     4921 caaccgtatc ttccacagtg gattcttctg tttcagctgt tgacagcaca actgaaaaac
     4981 agacacctga gccgtccctt acaattgacg gcaagtccat tgatactaag gactttatcg
     5041 tttgcaccat cgacggcaag gatattgatt ttgatacttt cagatactac tattattata
     5101 ctataagcaa gtacacttcc acttacggag caacccttga cactatcaag tcaactgacg
     5161 gtggttttga gcttcttatg gaggacgtta tcactgcact taagcaggaa cttgttgcac
     5221 ctgagcttgc agaagaaaac ggaattaagc ttacagacga tgacaacaag acaattgatg
     5281 accagatagc taaagcaaaa gcaaactatg attctgatga agcttatctt aatgacatta
     5341 agtcggctta cctcacagag gatctttaca gaaaaatgct tgaaacagct actatctata
     5401 ctaaagtaaa cgatactctt tttaagaata acggtaagta tgcaactaaa aaagaggact
     5461 ttaaaaaaat agtcaaggat acaaacgagt attgccgtga gattcacgtt atgatccctt
     5521 tctatgcaca ggtagatctt gatgacagta ccgctgacag ttatgacagt atgtcacttt
     5581 cagacaaagc gagcgcaaag cagtcagctt atgcgaagct tgatgatgac ggtgtaaaga
     5641 aagctaagga aaaggctaag aagcttgcag aggaagttct gaaaaaagct cagggcggag
     5701 aagactttga caagcttatt gaaaagtacg gctgggatct cggtcttgaa gatccttcaa
     5761 caggatatta tttcaataaa gatacgactg gatatcctga ggagcttgta aaagacgctt
     5821 tcaaactaaa agaaaacgac atttccactg aacttacaga gaatgacaca tatggatatt
     5881 tcataatcaa gcgtttgccg gttgatatgg attatgtaga acagaacata gacgatatga
     5941 tctattcata tgatacacct gctatctcaa agctttacaa cgaaagaatg gacaaaatgg
     6001 aagtcaagta ttgcgaccag tgggacaaga taactgcgga cagcataacc tgatgtatca
     6061 tggtatgatt aaagagcctg agcagatctc aggctctttt tatgcgtttt tgtgattgac
     6121 gtgagagtga atatctgata taataaaaac tgaggtgatc ttacggacag gctgaaattt
     6181 caagatgctt gcgataaaat cataggcaaa agccgtgaac gcaacggcat cggaactcta
     6241 ggcgagaaaa cgcttcatgc tgttttgaaa aactattttg agccatatgg tgaaaatcag
     6301 gagataccca ttggcggata tgtggctgat atcgtaggcg agaacggtgt tatcgagata
     6361 caaacaagac agttcaacaa actgctgaaa aaacttgaag ccttccttga ctactgtgac
     6421 gtgacagtgg tctaccctat ccctgcggtc aaatatgtcc gttggataga tactgaaaca
     6481 ggagagttgt cagaaaagca caaatcgccg agaaaataca ctatctatga agtcatgccc
     6541 gaactgtata aaataaaata cacccttgat aacccccgat ttcatttgtg cctttgtatg
     6601 cttgaaaccg aggagataag attgcttaac ggctggtcaa agaacaaaaa gcgtggatct
     6661 tcacgccatg acaggatacc aaagggactt attgacgaga tacaccttga ctgccctgcc
     6721 gactttgata tgttcctccc ggaggacctg ccaaaaaagt tcacttctaa agactttgcc
     6781 cttaatgcaa agatatctcg tgactatgca caggtaacgc ttattattct cacatatctg
     6841 gaaagagtga aacgtgtggg caaacagggt gctaatatac tttatgagaa aactgaatga
     6901 gcaaggctct ttacatacag tgcgaaagga agctgtcaat gaaactaaaa caaaaaatca
     6961 gcaggatatt atccatgacg ctggcaataa tgctgcttgt gcctgcgatg tgtgcagacg
     7021 cattcaattt tacacctaca tcaggctatg acgatgacgg aaaagcgata ccgttggagc
     7081 tttactctga agcggtctat atggtcaatc ttgacagcgg tgaagctatc gttgacataa
     7141 acggtgagga cgagcgtgtg cctgcttccc taacaaagat aatgactgct gtggtgcttc
     7201 tagacaaatt caaaggtgat gaacaaaaac tgaaagatac atactattca gcaggctcgg
     7261 aagcatttga tgaactatat gatactggtg cttcaacagc tgatatacaa ccaggcgaag
     7321 aagtaagctg ttatgatctc ctggcggcac tgcttatccc atcctcatgt gaagcagcga
     7381 atataatcgc tttgaatgtg tccgattcca taacagaatt tacagacctt atgaatgaaa
     7441 aagctgaaaa actcggcatg gaaaatacac acttttcaaa tgcacatggt ctgtgggcac
     7501 aacagaatta ttcgtcctgc aaggatatgg caactctttg tgaatatgcc ataagcaaat
     7561 atcaggtatt tcgtgatatt gtctgtctgc caagctatca tatggcatcc acctcttatc
     7621 actcagatgg aacagatatt tataatacaa accttatgct tgactcaatg accgattatt
     7681 actactccta tgctaaaggc ataaagacag gcacccttga ctccgctgga agatgtttag
     7741 cttcatatgc ggaatatgaa ggcaccacat atcttattgt gacaatgggt gcaccaatgg
     7801 ataagcttga agaagatgtg aagaagggcg aggaagatcc tgattccata tatggtggag
     7861 ataatgttta ctacaacctc cttgatcaca tcaatcttta caaatgggca ttttcaagtc
     7921 ttgtagcaac ggattttgtg gacaaagaca gcgaagtccg tgatgtaaag gtaagctatg
     7981 gtgacggcat agattatgca aatcttaaac ctgcaaatgg ttttacaagg ctgtggcctg
     8041 tagatatatc agtaaatgat gtggaaaaga aaataaccgt ttatgacaac gttgttgcac
     8101 ctgttgaagt tggcgatgtg cttggtaaaa tggaacttgt ctacaaaggt gaagtgcttg
     8161 caactataga tctagtttcc acaactaaag tagaacgttc ccaagtcaaa gcgaaggtta
     8221 aaatagctaa gtcatacttt gaatcgtctg ttttcaaggt cacattaaca atacttattg
     8281 cacttatagt aatatactct gtgatacata ttgcaaaaat acagaaaaaa tacatgaaat
     8341 aaaataagcg gtctgctcac tatgaattgt gaacaggccg ctcttttgcg tttactgatc
     8401 tttattttcg ttgtgatcgt caactatttc tggatcatcc tctctgttga cctcatttac
     8461 ctcatcagca cttggaatga ccatggcgtc aagctgttca ggcgtaagtg gaatacgctt
     8521 ttcatttttc ttatacaaat gctcaacatc ctccatataa tagtcggtat ctacagggag
     8581 cttcttcttt cttaaccgct gaacaacgct ttctcgtgta aaagtataaa gcaatgcaaa
     8641 agtcggaact gcaatgacca tacctatgaa cccaaacaaa tttccaaaga agaacagtga
     8701 tactagcacc caaaaacccg gaagtccagt agaatcacca agtatcttag gaccaaggat
     8761 attgccgtca aactgctgta gaataactat gaaaatgaga agtataatgg cttttttagg
     8821 atcaataaaa agcataagtg cagttgatgg tatcgcaccg attatcggtc caaaaaatgg
     8881 gataatatta gttacaccca caagaacact tatcatga
//
LOCUS       DAWEMR010000108         8761 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3330_length_8761_cov_10.731794, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000108 DAWEMR010000000
VERSION     DAWEMR010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8761)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3330_length_8761_cov_10.731794"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2546 a         1850 c         2063 g         2302 t
ORIGIN      
        1 ccctaaccgc tgtttgaagc agatgaccct ttttgcggtg aagcctaagc ttgatataat
       61 cggcacatat gtggaggagt tcgacactga cactgacgag gtagtttcgg tgaaaaaagt
      121 gcctgtcatg cataaggata tcgcttcata ttccaaacga cgcaaccctt tcaacaggca
      181 gtctgtggct tttcgcaagt cctttgcgga aaagataggc ggttacagtg accttaaaat
      241 gtgcgaggac tatgagttta tcgcacgaat gttacagcat ggtgcaaagg ggcagaatat
      301 acccgaggtg cttgtgcgct accgcataga tgaaaatact gtagatacac gcaaaagctg
      361 ggagcataca aaggctttca ttcgtgtgag gtggataata caccgttcag ggtatacagg
      421 cttccgtgac tttttcatgc catgcgctct gcagcttggc ttgtttatat ttcccagaaa
      481 attcacccgt tgggtataca gaacgtttct cagaaaataa tattttttat atatgaaaat
      541 atgaacccga tgccgtgtgg gttcatattt ttttaattca aataaaagta aacgttatca
      601 attttgaaca cgatgttaaa cattaaaaat ggaatataaa gcctaaaggc gagagcctcg
      661 caaagcttgg ctttataaaa atttgaatat tttaattagt aatgataagg agaacattat
      721 gcgtgaaata atgaaaagaa cagttgctgc cgttatggca gtgcttatcg cttgtgcggc
      781 agcaggctgc agcagcacag gaaacagcag ctcaggcagc tcgtcagcag gcgaaactac
      841 cactacaaca gctcctgtcg ttgaagatat gggcaacatc gacctttcag acgtaacgac
      901 agagtatgac gttcctgagg atttcagcta cgaatccgag gcggaaaaag gaactctttc
      961 gggaggtgcg gcagtgcttg ataagaattt tcttggtaag ttttctggcg atggcttcgt
     1021 ttccataggc tcatcgggag atatggtgga gttcgagata gattttcctg caaagggaag
     1081 ctataatatt actctcacaa tggcggcaga cggcgagggt cagtcaaatc ttatcactgt
     1141 tgatggtgct gcgctcacaa acttcacatc aaccacaaca gagtttggcg acactatggc
     1201 tgaaaatgtg cttatcgacg aaggtactca taaggtaggc ataaagggcg acaacggtca
     1261 tatctatatt gacaagataa agataacccc ggctcctgct atcgaccttt cacagtatga
     1321 ggtatcaaat cagctttcaa atcctaacgc ttctgacgag gcaaaaagac tttataactt
     1381 ccttacggac gtttatggca agtatactat ctcaggtcag ttttcaggag ataacgaggg
     1441 caaggactgc cgtgagttca aggagataaa gaagcataca ggcaagacgc ctgctatact
     1501 tggtcttgac gtttcaaacc tttcaaacag cgcactttca catggcgcag gcggcggaga
     1561 tatggtgcca ttgcaggcta tggactggta caacaacgaa aatggtatag tttcactttg
     1621 ctggcattgg tatgcccctg ataaaaacct ggaaaagaac ggcggagcat ggtggcaggg
     1681 cttctattct gaatacacag acttcgacct tgcaaaggct ctcagcggtg aggacaaaga
     1741 gggctacgat tctatcatag cagaccttga ccatatggca ggggtactta aagagcttgc
     1801 agacgcaaac gtgcctatac tttggcgacc tctccacgag gcggcaggcg accctaagta
     1861 tcctggcaac gcatggttct ggtggggttc agcaggaaag gacgcatata ttcagctttg
     1921 gcagctcatg tatgacaagt tcacaaacga gtatggtctt aacaacctca tctgggtatg
     1981 gaacgcacag aatcctgact ggtatccggg tgatgagtat gttgacatca tgggctatga
     2041 ctgttatcct gcagagcagg acagctcttc acagaagtgg tattatgacc ttgtgaagtc
     2101 aagcacatcc acaaaaaaga taatcgcaat gacagagaac ggctcgatgt tcgaccctga
     2161 cggagcattc aacgacggca cacgctgggc gtggttcagt acatggaacg gcgagttctg
     2221 tatcaaggat aaacagcttt cagatcagta caccacattt gacgagtgga acaagatcta
     2281 caacagcgaa agagttctta cccttgacga gcttccaaac ctgaaatgct atccaatgga
     2341 tactgaaaag tatctggagg agcataaata atttcggata ataacagtag gggcgaacag
     2401 tgttcgcccg ttactttgca cattttcgac caattcaaac aggcttgaac tcaacttaca
     2461 aaaattgttg atatcatggc ggcaatatgc gggcgaccta agatcgcccc tacataacaa
     2521 aactgcggac agtgtttaaa ctatccgcag tttttatttt tatcattaca atgcttgtgc
     2581 caaaggcggg cgaacgctgt tcgcccctac aatatagacc ttgtggagta ttatcagcta
     2641 taaatattca gtatgcaagg tgcaacataa accactatca tcacaatggt tcccgcaccg
     2701 ataacagcca caagtgcagc ggcttccata gtttctccga atttcagttt ctttcttatg
     2761 aagaaatatc ctgctgtcag cgcaatgcct gcgatagtca gtgcaagacc tgcataaaag
     2821 ccatttggca cgcttgttac tgttattgtg ttctcgccct cctgcaagtc gaagcttaca
     2881 aggtcaccga aaaccttgtc atatgaaaca gccttgccgt tgacctttat tttcagaccc
     2941 tcctgatatg gtatctgaag cacacatttc tgtcctgcct ttgcgttcac gcttcctgaa
     3001 agcttgccgt cactgtctgt aagaccggcg gtctgtgcct gctcaagtgc cttttcaaga
     3061 acattgtggt gaagcccgaa aacgccatag gaacggcagc ttgtgatgtc tttcttcaag
     3121 gtcacacgca cgttgacgtg ttcattttca aattccccaa gcttcaaaag cccgttgctc
     3181 gattgtgacg gataatccat ctgctttacc tgaccgttca ctgttaccat gaagcttccg
     3241 tttatatcct cgctgaggct gtttgaaagc ttgtcgaagc agtcaaaata aagtgtctgc
     3301 ttgtctgtca catctatgct gtagtccatg taggctacat cgctagtagt agtgagggtg
     3361 tagttcggat taaagttact gtcgtcctgc acaccataga tcgtggagta ttcgtagtct
     3421 gtgataagct cgttaccgtt gccaccgaaa agctgttcaa aaagcttctt ctgaacgttg
     3481 cttcttgtgc ctgtggaaag ttcctcactg ccgcttaggt cagcgtcggt cacaatgcca
     3541 agggggagat agtattctgt cggcactatc tcgtaggtat cgtttgaata tacagcgtca
     3601 gccgagcctg tacttttttc aataagatat tttactgaca gcaaagcgtc agtaagctcc
     3661 gttccgccat atgagcctac ttccatccag taggaggagt aaccaagctg cttcatcata
     3721 tacatataat cacggcttgt gagggaggtg tagtggctca gggaattgta gcccattgaa
     3781 ccgaccagat tgacctcgaa aagcttgctt gcggtcttta ctctgtaaaa gccctctgtt
     3841 tcgtcaagtc tgtctgaaag atccattatc ttctgatgat tagccgcacg ctgaggattg
     3901 ttcatgcttg gggataccat ataaatgttc acgtttgcgt atgactcagc cgcaacgaac
     3961 attgcaagga agatcgcaaa tatcttcttt gatatccagc cctttttgtg tagcagaacg
     4021 acaatcgcat aaaatgccgc cgccacaatg aaaaattcaa ggcaaagctc aaaggcggtg
     4081 ctgtcctgcc aaaggcttga ggtgtatttt gatattcccg agcggttgtt ttcaacaaag
     4141 ccctctgaga agttgtaaaa gccgtagata agtgccgcaa gtattaggaa gataacaaag
     4201 tggtcgcagt ttttctttcc ttttgccact gcgttttccg atgaaaggaa atatcctgcg
     4261 cagataagtg caaagaaaat ggtgatgaaa gcgtatctgc atgggaatga catatagctt
     4321 cctgtgtgcc acataaggtt cgcaggctct attaaaatag gtacgagcat caaaaagagc
     4381 attataaggt cggtgttcaa acgcttgctt ctctttttgc cgtcaagaag aaacaccgca
     4441 actatcacta tcaccgaagc cgttgacaac agcaggcaga aggtcgtgta gtagtttgtg
     4501 atgaatgaag aagtctcaag ctgttcgata actgactttg aacgtccgct tgacaaaaac
     4561 tgcaggaagc atggtatcca taccaccgcc gaaagcaatg caccaaggag cgagcctata
     4621 acaaatctgc atggaacgtc cctgtatttt tcctccttgc ggtaacgcca gcagcaaaga
     4681 gccatgaaaa tcattatgaa aacaactatc atgtagctga tatagtagtt cactatcatc
     4741 ataagtgtga gcatgacagt gtatgggata atgcgctttt tctcgaaaag ctccctgaat
     4801 gccaccataa ggagagggaa aagatacatc atgtcaagcc atatgttgtt ctgataatag
     4861 agcattccat agccgcagaa tgcgtatatg acgctgagta cagcgccaat gtaagggtcg
     4921 agcttttttt gacaggagcg gaaatatatc tgcgccgtta cagccgctgt catcatttta
     4981 agcactgtga gaatgttcac aaggtagatg atgtcagcct tgtcaacaaa aagcacaagc
     5041 aaggtgtagg ggcttgcgat aaagaagaag aaaacgcccc acaggtccat tcctcctgca
     5101 ttgtggaagt taaggaacat tccgtccttg cctgtgaaaa tatccttgaa gtcggtgagc
     5161 agaggtatca cctgctgtga catatcacac catgatacag tgccgtcacc aaaagggtaa
     5221 agcccatgcc gataaaaagc aaatcccatt atgagcaata cagccagagg tgggatgata
     5281 aagggcagag cttttttcag cttatccatt ttgttcagtc ctccatacat aacatagttt
     5341 ttatattttt ctttaaagaa acattataat tatactccat taagagccaa aaatcaatat
     5401 taaataagcc aactttaggc ttcgtatgat gaaaattctg tgaaaaacat ggtctgatgt
     5461 tgattttttt gcagggaggt gctataatag gctagtgaaa aggcagaaat gccataaacg
     5521 ataattgaaa ggacgataaa aatgaaactt aaaaagctat tcagagcatt ggctctttgc
     5581 acagcaggcg tgcttgcctg cgcaattctt acagcctgcg gagataaggg tgacagctca
     5641 tcacagacct ctgcggctga cactgacaag aagttcgtta tcacgcttta tgccaatgac
     5701 gcacctatca cctgcgagaa cttcgagaag ctggtaaaga aaaagttcta tgacggactt
     5761 acttttcata gagttgtaga cggctttatg gcacagggcg gcgaccctaa cggtgacggc
     5821 acaggcggaa gcaaggaaac tatcaagggc gagttctccg caaatggcgt tgaaaacagc
     5881 ctgagccata caagaggcgt tgtttcaatg gcaagagcaa acgatatgga cagtgcctca
     5941 agccagttct ttatctgcta cagcgataag gatactttcc ttgacggaca gtatgcggct
     6001 ttcggcatgg taacagaggg catggacgtt gttgatgact ttcttaaagt tgagagaaca
     6061 acaggttcag acaacgcaca gtcaaagcct gttacgccta tcactatcgt aaaggctgaa
     6121 atgatagatg atgacgcaga cggcaaccac agagttcagt tcacaatgga tttctaagct
     6181 gattttcaca aagaggtgtc cccattgggt ggataccttt tttgtttact aaaatggttt
     6241 ggcaaaacaa aaaattcata atttatatat caaatcactg ataaaagcta ttgcaaaaag
     6301 ccgtgtgttc agttataata tatgttatag gtttatgcac agtcggtatc gtagggctgt
     6361 cgctatcaat ttttgaaagg aatgtatgtt atgaagaaat ttgcagcagg aattattgca
     6421 gtggcaatga gcgttacaat gcttgcaggc tgcggagtgc ctgctaatac agttcacagc
     6481 gttgacgatc tggagggcaa gactatcggt gttcagcttg gaacaacagg cgacatctat
     6541 gccgaggacg ttaaggacgc taaggtagag aaatacaaca agggcgctga cgctgttcag
     6601 gctctgaagc agggcaagat agacgctgtt ataatcgacg ctgagcctgc taaggtattc
     6661 gttgagaaga acgacgacct tgaaatactt gacgagcctt ttgctgagga agaatacgct
     6721 atcgctatga agcttgacaa cactgagctt cagacagctg tcaacggtgc tctcaaggag
     6781 cttaaggctg acggaacgct tgacgctatc aaggcaaact acgttggcga gaacgctggc
     6841 aagaacccat acaagtcacc tgataatgtt agcagagata agggtacgct tgtaatggca
     6901 acaaacgctg agttccctcc atacgagagc aaagagaacg ataaagtcgt tggtatcgac
     6961 gctgatatga tgcaggcagt ttgcgacaag cttggttatg agcttaagat agacgatatg
     7021 gcatttgatt ctatcatctc agccgttcag tcaggcaagg cagacgttgg cgttgcaggt
     7081 atgacagtta cacctgacag agagaaaaac gttcttttct ctgacactta cacatcatca
     7141 agtcaggtaa taatcgtaag aaagaaataa tttttcacac ttcggctgtc gtagagcggc
     7201 agccgaaatt attttgataa agaacaggaa gtgctgaatt gaacaaattg atcgacgatt
     7261 tccagcaaaa ttttttggac gagagccgtt ggcattatct taccaatggt cttaaaacaa
     7321 cacttatcat aaccttcttt gcggtcatac ttggctttgt gttgggattt cttgtggcta
     7381 tagtccgctc tactcatgat aagacaggca aaatgaagat actcaacgcc ctttgcagag
     7441 tgtatcttac agtgataaga ggaactccta tggtagttca gctccttata acatattttg
     7501 ttattttcgg aagtgtgaac atagacaaga ctcttgtggc tgtgcttgca ttcggcgtga
     7561 actcaggcgc atatgtggct gagatagtcc gttcaggtat catgtcagtt gacaacggtc
     7621 agtttgaagc aggaagaagc cttggcctta gctatcgtca gacaatgata tcaatagttc
     7681 ttccgcaggc ttttaaaact gttctgcctg cccttgcaaa cgagtgcata gttttgctta
     7741 aagaaacctc tgttgcagga tatatcgcaa ttcaggacct tacaaagggc ggagatataa
     7801 tccgttctca gacgttctcg gcattcatgc cgcttataac agttgcactt atttacctta
     7861 taatggtagt tgtgcttact catctggtag gaaagcttga aaggaggctg tcgcaaagtg
     7921 atagacgtta aacatctcag caagtctttc ggcgaggtcg aagtgctgaa agatataaac
     7981 gaaactatcg acaagggcga aaaggtcgtt atcgtcggac cgtcaggctc aggaaaatcc
     8041 acctttctcc gctgtcttaa ccttatggaa aagccgtcct caggtgatat attcttcgag
     8101 ggcgagaaca tcactcaggc gaacgagcat gaaatgaaca agctcagaca gagaatgggc
     8161 atggtttttc agcatttcaa cctcttccca catcttacaa taaaaaagaa tatcactctt
     8221 gcccctgtga agcttggact tatgacagcc gatgaagcag acaaaaaggc ggaggagctt
     8281 ttggaaagag ttggcctgcc tgataaggct gacgagtatc cttcaaggct ctcaggcggt
     8341 cagaagcaga gaatagcaat cgcacgttca ctggcgatga accctgaagt aatgcttttt
     8401 gacgagccta cttctgcgct tgaccctgag atggtgggcg aggtgctgga gcttatgaaa
     8461 cagcttgcaa acgacggaat gacaatggtt gtcgttaccc acgaaatggg ctttgcaagg
     8521 gaagttgctt caagagttct gtttatggca gacggaaaaa tactcgagca gaacaagccg
     8581 tcagagttct ttgcacaccc tgaaaatcag cgtttgaaag attttctctc aaaaattata
     8641 tagaagccaa caccgcacaa ggggcgtcta tcgtctttgt gcggtgtttt tttgtttgca
     8701 aaggtctaaa tgtaggtttg tcaatcatat tggacactat tattaagaaa ttcgataggc
     8761 g
//
LOCUS       DAWEMR010000109         8742 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3339_length_8742_cov_10.131921, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000109 DAWEMR010000000
VERSION     DAWEMR010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8742)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3339_length_8742_cov_10.131921"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2560 a         2019 c         1877 g         2286 t
ORIGIN      
        1 tgctcacgag cattgtttcc acagtggcta gtgcatcggg cggaaacgag ataaagctca
       61 caagcgacaa aaactttgca caggcaggcg acagcataaa cgtaaaagcg agccttgagc
      121 ctgataaaac aggcgttgca ggcttcacta tagacctgca ttatgacccg tcaaaggtaa
      181 cagtatatgt gccgaccgat gaggagagcg agagtacata caatgtggga agcaaattct
      241 ctgttataac caactatgtt gcttcatcag gcactataaa aatagtcggt gcaaacctca
      301 caggttctaa cataaaggac agcacagacc ttgcccttgc gactttcaag gtaaaagacg
      361 gcgtaacagg caagataaac tattgggtag aggttgaaac aatggtggcg gaagctgacg
      421 gtggatacaa gtcagtttca tactcagcac ctacagaggg ttcaccgctc actgtcagtg
      481 caggggagga gatcacaaca actcctgcaa cgaccactac gactgcttca tctaaggctg
      541 aagctactac aactacatca aagtcgacca caactacaac gacgactaca gcaaagacta
      601 cgaccaagtc cgaagcgact accacaacta cggctaagtc tgagaaagtt acaacaacta
      661 caacaaagcc tgttgaaacc accacaacga ctacaacagc aaagagcgaa actcagacca
      721 ctacaaccgc aacatctgca cagcctgagt cagaaccgct tttcacacac aagcagggtg
      781 acgctgactt caacagcgaa acttcccttc agttcggctt caagctttct gactacataa
      841 cagactattc tcagcattac aatgtaaagg tcaacgtaaa gtcatcaggc aacgcaagcg
      901 gtgcagtcgg tgcgttggta gacggaagct ggtctgcaca gaatacgaag ctcacaggtg
      961 gcgaggaagc acagtggata tactcagatc ttgacccttc aagaacaagt gacgaggttt
     1021 tcgtacagct ttattactta aaggcaaatg cagattttca gatagcttcc gtggaagtaa
     1081 cgcctgtcaa ggaagcagtt gacacatcag ccgttacaaa ggtcacagcg gcagaaccaa
     1141 agcctgctga cgatacaacg accacaacga ctacaacagc aagcacagag ccaaaggctg
     1201 acgaaaacac acagcctgaa agcaccacca cgacagcagc cagacagcct gaaaatgtcg
     1261 gtgctgctgt tactacagta agcgaggacg acagttcgca ggagcttaca gaatcacagg
     1321 caacagaaaa gatacagcag atagttgacg aggcctcatc gcaggctgac agctcaaaag
     1381 taaatccgtc aacaggcgtt gaaatgacaa agaacattct cactgtcatt gccgcaggat
     1441 atgttatttt tgcgatattt gcagttatct tcaacagatt tgccgcaaag gacgaagaat
     1501 aataaccgaa tatatttctt ttggctataa aaacggacgt ttccatcgga aacgtccgtt
     1561 tttcgtcagg aacaccaagg ggatagtatg atcctgcatt ttattgatct tctataacag
     1621 gcggaatgaa attctctaag gcgctgagaa gcttcgggtg tcgtataaca aaatgtatag
     1681 caggcgcgtt tccctttgat atcatcgccc actgaccttc atggatcagt atttgcaaat
     1741 tgcagaaggt ctgccgctta gcatatttaa ggctatagtt ttcatgtgtc tgtgcgtagc
     1801 gttctgtgct ttttaaatgt gtagaataat catcatagct gtaaagaaca tcactggcac
     1861 agaatacacc cgacaggtca agggcatggg gacgtgtttc aaattcctct cgggaaagac
     1921 agcatatctc atcctctatg gtcatggttt ccagtatgct ctcaactcgc tttttacgat
     1981 ctctgacata agcttttata tctctgcccc agcggtcgtc aatgccgttt ctgtcaagta
     2041 tactattgag caggtcatta tccatagtat aaataggcag agcagataaa atactcctgc
     2101 gcccgccacg tctgtgagag tcggcagtga gaaaatcgct gaaatctttt tccctgtcac
     2161 ttcggtatat ctccataagc gggcaggcgt ttgaaagcag atcattggca cgtttacgat
     2221 aatatccaag ctcctttttg gacttggtca gataatatct gccctcgctg tgaaagcctg
     2281 ccacggcttc ccctgaaaga gccgccgcac ctgatacctt cagcaaatgc aaaaaagcat
     2341 tattctgtac atttttcaaa tagtaaggcg ctatctgccc cgtcatatac atcggtatcc
     2401 agctttcaag accaagcatc atatcatcta gggagcggtc aagattatgt atctggcaaa
     2461 ggtgcagacc ctttttaagc atcagagcca taccgaacat ccacttcttc ggaaattcgg
     2521 gatcatcagc catttccttc ataggcatat cactatacat tatcacaggc tcattagaac
     2581 gtgacaagac tgtggctttc agaaaatcaa gctcgctttc catcatttcc ttgatgccaa
     2641 aataactttt tgacacagga agctgaaaag gcaatgcagg tactttcatt tcatcaaaac
     2701 gtatcgcctt tatgtattcg ttcaggtcaa actcatcaaa cttatcaaga aactcttcaa
     2761 ttccaccgct ttttatctct ctgctctgtc tggcatgatc gctgaacagc catttgcata
     2821 tcacttcata acgctgtgac acatcctcta tatcactttc attacagccc acaagctgtg
     2881 cgaccgctga tatctctgtg ggagttctaa gctcccttgc agtaaagcct gctactgcgg
     2941 cgacaaagcg ttctgggtcg gcaggctttc tagtaccgtt ccgtatacgg aaaacagtgg
     3001 aaacatcata atttatctcc ttgcacatac gcttgatgtt tatatcaagg gctgagataa
     3061 gggtattgaa attttgccgc atagactctc tgtcggcagg tatgatatct tcacagtcaa
     3121 gaaaggctct ttttatgttc tcccttgtca tatcgccata gcctttttcc cttgccacag
     3181 cctcaagagc gttgcaaagc ccgtcaaacg ccacagtgtc tatctcaggt attctctctc
     3241 cgcttctgta tctgctaaat gttgagggag atatccctga caactcacaa agctcctttg
     3301 ccgtgcagga aagctgttct atatacttac caagctgttc gcaaaacttc attttaacga
     3361 tccctcccgt tgtcatatgg tattacgtta attatatatc atatttatca aaaaaggtag
     3421 tcccccacga tatataattt cagaattggc atatgactat tatgaaataa atcacaaatt
     3481 tattcataga tttgttataa tgtgataaag gatatcacag tagggaaaat acaatgtaat
     3541 ggaggtgtgc ttataggata gtagcgaaca tgagaaaaaa atataatatc aaaaggagat
     3601 gtatattatg aaaaaacgat cactgcaagg gagaataacc gcttttatcc ttaccctatg
     3661 tttggcagca ccacaaatgt ctatgttgac atttgctgaa aattctacag tcagtaatga
     3721 aacggaactg aagtcggcat tggaaaatac ggaatttgca gagatcaagt tgggcggaaa
     3781 cattgagacc acctgggaac tagatgtgga gcgcactgtt actcttgatc tcaacggata
     3841 taccctgagc tgcagctcga cagatgagga tataattcgg gtgcgcagca gcggaaacct
     3901 aacagtaaag gacagcggca ccaatggcaa gatcgatggg cagaacaaga actgcggatt
     3961 tgaagtcaag ggcggaacgc ttactctaga gagcggcagt atcgtaaatt gcacagatgc
     4021 cgacggagac ggcggcgcag tggatgtatc caacactggt gtaacggaaa ctccggtcaa
     4081 gtacggcaag ttcgttatga acggcggtac tattaaggat tgcaaagcag gtgatgacgg
     4141 cgctgctgtg gatataggca tgggctgcac atttacaatg aacgatggta cgatcagcaa
     4201 ctgcagagcg gaggacgacg gcggtgctgt atttgtcaag cagggcggct tcttcgtgat
     4261 gaacagcgga acgattgaaa actgttctgc atgcaataat ggcggtgcag tgaacatata
     4321 cgaaaatggc agcttcacca tgaccggcgg cacaattaag gactgcaagg tagacttggg
     4381 aggtctcggc aatgcagtat atggcaaaaa caatacggcg atcgttgtca tttctggagg
     4441 tacgatcgag aactgtggtg tatttccatg gtcttttgat gagttcacag tgacatttga
     4501 ttcagacggc ggaagtgcag tgtcagagca aaaggtattg aatacttctg cgatcgagcc
     4561 aaatgaaccg aagaggaacg gctatgattt tgctgggtgg tatctaaatg atgcaaagta
     4621 tacctttgat gccaagatca ctgggaatat tactctaaaa gcacattgga cgcctgcggc
     4681 aactttaagc agtattaaca tgatcgatat cgaaaacgtg aagctcgatt atcagccggg
     4741 taatgccccg caggcatcgg ccaagcgaac aggcactaat caggataaat atgatattct
     4801 ctttgagtgc tgggaaaagc aggaaaagga tgccaacgac accatgcaca cggttggcta
     4861 ctggtattct gatgaaagcg tttattcgga cgacgatgtt cgattcaata cctttgaaaa
     4921 gggcggaagg tatatatatt ccgtgaaact tcaggcaaag gacggctata ccttcgatcg
     4981 caatttgaaa aatgaaaatg ttacactgaa tggagcaagc ctgccgtcta gcgcgtgggt
     5041 catggtgatg gatgacggca agacctgtct tattagatat ggaacagagc tgcgccccgg
     5101 tcaggctgtt gaagaaatcc gacttaatgc cattatcaac tttaatgccg gagataaacc
     5161 gcttttcagc accggtgtca ttgaccctat cgttgacacc gatcatcagc ggtgggacgc
     5221 caatgacggc agcggttatg gcattacctc cagcgactac tggaacgaac gctataacgg
     5281 taagctcatt acggaatttg aagccgacaa aagctatacc tatggcgtat atttcaagat
     5341 ttccgatttg ggcatggaag agggctaccg cttcgatcag aacacgaagc tctatatcaa
     5401 cggcaaaaaa atcaccctga cacccgatca gatcgatgtg gatgatagcg gtgaaaccat
     5461 ttggttcagc aatgtattga ccatgacgcc gtccaccgtg aaagttattg acgtggtgga
     5521 gataaatgat gtcaccatca acttcaagga cggcgacaaa cccgtgttta ccggcaagag
     5581 tcctgaaggc gtgaagtatg catacaattg cgagtggtgg gaattggaca gcaagacagg
     5641 tgcgatctct gcggatttct tcagcggcgc ttatgaaaat aagataacca cttttgaaag
     5701 tggaaagaca tatcattacg gcgtgtatgt gaaagctgtg ggctttgttg agagtgaaaa
     5761 tacctcttac atattcggac ccgacacgaa gctgaagatc aacggcgaat ttgtaaatta
     5821 caaacgctat gaaaatgata cgagcgacgg ctctgacggc accttgtggg tcatcactga
     5881 ccttactatg acgccaaagg caaatgatac aagttctgac aacagcgata caaactcaga
     5941 caacaaagac acaagttcaa acaacagctc aaatacaaac agcaactcaa acactgacag
     6001 caagaaccct gtaaagaaca atgcaggaag cacaaatcct aacacaggca atgaccttgg
     6061 cggtatcctc tttactatcc ttatcacagg aagcggactt gtgatcgcag gcagaagatc
     6121 acgcaaaaac aaataacaac atgagctgac aaaaaacggg cgtttcaaag cgaaacgtcc
     6181 gtctttttat atccatatca aatttactta ttctgtgcct taaagataac gcccgttacc
     6241 tttggacctg cgtttattgc aattgccgca cctatttgga aacactccac aggtggatag
     6301 cccaccttct tcgtcatagc ctgtgccatt tcgtccctga cgctctcgtc attgccgtaa
     6361 acgatgcagt aaggtgtctg cggtatcatt tcctttacag tcaggtcaag tatctttggc
     6421 acaatagcct tgtcgcctct tacctttgtt tctgtaacga tagcatggtc atgtatcctc
     6481 atgataggtc taaggcccat tacctcgccc acaaaagcag ccgcagacgg aattcttcct
     6541 gacttctttg catatttgag tgtatatggt gcaaagaaaa taacgcagtt ctttatccag
     6601 tcctccataa atgccacgat atcagccgca ggaacgtcct ttgtggcttt cattgcgccc
     6661 tgaatgactg cgtagccata tgcaccagtg tagctctcgc cgtctatatt gtatatagtg
     6721 aacttgccct ttgactcagg gtgagcggaa taaaactcct ctgccgccat aacagcgttg
     6781 ttgtatgttg ccgagccttt tgagtttatg gtagtgttta taacatcagt atagccctcg
     6841 ctgtagagct tctcaaaagt ttcctgatat tcaaaagctg ttatctgcga tgtaacaggt
     6901 atgccgtcaa aatcgtcaag cattttgtag aatttctcgt tatcgaaatc tatccttgat
     6961 gtgtagctgt catcacccat tgccaccttg aaattcatta tctggatatc aagcttcttt
     7021 tcaagctcct cagtgatatc gctcgctgag tcggtaagaa attttatttt tgccataaat
     7081 tcaccctccg tttttttatt tattttattc gtcccatgta tattgcgtca gcttgccctc
     7141 tctgccaagg tcgggataat tccactgtct taacacctca tagcaggcta tcgccactga
     7201 gtttgacaga ttaaggcttc tgagcgtttt cctcattggt atccgcacac agcttttggg
     7261 attttcataa agcagtttct caggcaggcc cttgtcctct ctgccgaaaa ttatatacac
     7321 ttccctatcc tgcggataat ccacatctga atgtaccatt ctgccctttg tggtaaaaaa
     7381 gtaaaactca ccactatttt tggagaaaaa gtcgtcaagg tcgtcatact catgtatctc
     7441 cagcttatcc cagtaatcaa gccctgcacg ctttagcttt ttgtcgctta tctcaaagcc
     7501 gtaaggcttc acaagatgca gtgccgcacc tgtcactgca caagtccttg aaatattgcc
     7561 tgtgttctgc ggtatctgcg gctctacaag cacgatatta agtctgttca tcttaggtaa
     7621 cacttcctga tatcacaaat tcgtatagtc cctaagctcc tgatagcttg taagccaagg
     7681 aagctgaccc tccagcataa gaccgtctgt ccattgggtc tggtagctgt atgtagtctt
     7741 gacagtaagc tctgtgaacg ctgtggctct gttcattgcg ataggcaggc tgtcaccctt
     7801 gagaagtcct ccaataagga cacttgtgaa catatctccc gttcctgaat agtgtgcagg
     7861 aacataatca ttctttattt tccaaaagct gttattttct ttatcatagc ctacagttga
     7921 cattccgccg tctgcaagat ttacgcttgt gataacaacg atctttgcgc cctgctttga
     7981 aagccttaca agccatgatt ttatctcaga gctgcgcatt gaagccgtag gatattcctc
     8041 accaagaagt atagccgcct ctgtgaggtt aggcgtgatt atatccgcca cctttacaag
     8101 ctcgaccata cggctgcaaa gctcctttgt gtaagtctta tatgcctttc caccgtcacc
     8161 cataacaggg tctacgacct tcagggcgtt aggaaaagcc tcgaaaaact caaggcagtg
     8221 gtctatctgc tcgcttgaag ctagaaaccc gctgtacaca cagtcaaatt tcatatccat
     8281 ttttttatag tgttccaatg caggcttcat aaagtctgta agatctctca taacaaactc
     8341 gctgtagccc gtgtgtgccg aaagcacagc cgtaggcaca ggacacacct gaatacccat
     8401 agcggaaagc acaggtatca caactgttag cgaacaccgg ccaagacctg atatatcctg
     8461 aatggaaaca acctttttac aaccctgcat aataaaaccc tcttaaaaaa tactcataag
     8521 cattattata acatatccat caggattttt caatagccca tggcttgccg gcaatatttt
     8581 tttcactata ttttttcata ttgttcatat caatataata tgatttacgc gctttatgaa
     8641 aagcaacaaa aaagtgccat ttttcgcact ttttagacta gcacctacaa aattcatcat
     8701 gataatttgt acaaatttag tcttgcaaaa acgtttaaaa ta
//
LOCUS       DAWEMR010000110         8616 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3393_length_8616_cov_9.085387, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000110 DAWEMR010000000
VERSION     DAWEMR010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8616)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3393_length_8616_cov_9.085387"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7182..7191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2698 a         1701 c         2039 g         2168 t
ORIGIN      
        1 ttacaaaaca gaaacttaat tattacagat tgtaataatt aatgatgtat aataatataa
       61 atgaaatcgt ataagtacat aaatacaaaa accggaaagg gttgatccat tatcaacata
      121 ataagaaaaa taacagcagt gataatttca tcagttataa tgataagctt tgtggcattt
      181 gcaccgcagg gatccgctaa gacttatgtg aatgttataa cagcttctgc tgctgactcg
      241 atgacaaaac ctgttttgat aaaaagacaa gcgtattcaa acaaaataga agcaggagta
      301 aaaaatctca ccagcttttc atcaaagact atatttgagg tctatgtaaa cggacgctat
      361 gtaagaaaat atacatggca ggcacttaaa aaggataact atataaatat aaccgatgac
      421 ggcaagcttt atcttaaagc ttccacaaaa tataaagtct ccgtcaaagc ggtaaacggc
      481 agtgccaagt cagagtcatc ggtcctcaac gtaaagacgt catcaaaaac atactattat
      541 atagataaaa acgttcagct ttatagcttt aaaaacggta agttcaagaa atcctcaaaa
      601 acaaaagcct ctgtggctgt gtcgggtact ctcactaccg ataaaaataa gcgtgtggca
      661 ggtaagagca agaatatcgg cggagcaaat tatgtcctta tcaatgaagg cgatcataag
      721 ggtaagtacg tcaaggtaga aaaaggtgtg aggcgtacac ctgagagaaa agcaagaata
      781 aaaacagccg ttgattatgc cgcaagcatg aacggcggaa gatatgtctg gggcggaaca
      841 aagtataagg caacggactg ctgtggattg acaatgcaag cctatagaaa agccggtgtg
      901 aatatgtata acagcgtata ttcgcaggcg aaaatgggaa aagcagtgtc tttgaagaat
      961 atagaagcag gcgacctgat aatctgtaat aactatggcc atgtagcaat gtatataggc
     1021 ggtgggaaaa tagtccatgc catgagtaca tattatggga tcagaataca gcctcttgca
     1081 aatatcaagt attgcggaaa gataaacact atcaggagaa tactgtaatg cagagcgaac
     1141 ttgcggcata acaaactgtc gaataactgt agtggcgacc tttggtcgcc cgcttggttt
     1201 catagcactg tatcggcaaa ttttcaaatg taaatgtagg ggctggcgtc ctcgacagcc
     1261 cgattttgtt tgctcgataa tacttgcgat atctgcacaa tttttccttg acaatttcgc
     1321 tgaccagtgt tataataaaa aagcagaaaa gctgcgaaaa atcgaataaa gtttgcaggg
     1381 ggacccaagc aaaaagctgg gttgagaagg taaaacctga ccctttgaac ctgttggtca
     1441 agaccagtgt aggaagcaat ttgtgtaaat tttacctcct gcatggtcag gaggtttttt
     1501 tgtttcccct tttccataag aaaggaataa agtatgttaa aatgtttgtc agtggcaggc
     1561 tcggattgct ccggtggcgc aggtatacaa gccgacctta aagccttcag cgctaatgaa
     1621 actttcggca tgagcgttat tacctctgtc gtcgctgaaa atactacccg tgtgctgtct
     1681 gttttcaacg tacccgttga ggagatacaa aaacagatag acgctgtgtt tgaggatatc
     1741 cacgtcgatg ccgtaaaact cggtatgctc ccaactgccg aaattatttc agcagttgcg
     1801 gataagctta atgagtataa accaagtatt atcgtctgcg atcccgtcat ggtcgctaca
     1861 agcggtgacg ctctctctga gggcggaact gtttccacca tgagggaaaa gctctttcct
     1921 atagttacgc tccttacacc gaatatcccc gaagccgaag ctatctcagg cttggagata
     1981 aagaatgaaa accatatgga aaaagccgca aggataataa tggatatggg cgtaaaaaac
     2041 gtcctcgtca aaggcggtca tctcactggc gacgctgtgg attatctctg taccccagag
     2101 ggcgttcata agcttgcctg caagcgtgtt gattcgccta atacccatgg cactggctgt
     2161 actttgtcat ctgccataac tgcaaacctt gcccacggta tggatatgct ctccgctgta
     2221 acaaaggcga aaaactatat caccgaggca atagaaaaat cattcacagt gggcaaagga
     2281 catagccccg ttaatcactt ttatgactat tacgatttga aaggaactgt tctatgagaa
     2341 attataaaac tcaaatggaa gccgctaaaa agggtatcgt tacccctgaa atggaaacag
     2401 tggctaagaa agaaaatata gacgtaaacg agcttatggc tctcgttgca gcaggtcagg
     2461 tggcgatccc tgccaacgta aaccatactg ctctctctgc cgagggtatc ggtaaaggtc
     2521 ttaaaactaa gataaacgta aatctcggta tctcagggga cgctaagaat tatgacattg
     2581 agatgcagaa agtggatatg gctatcaagt tcggcgctga agctatcatg gatctgtcaa
     2641 actacggcaa gaccaataca ttcagaaaag agctagtagc aaaatcgcct gccatgatag
     2701 gcactgttcc tatgtatgac gctatcggct accttgataa agacctcctt gagatatcag
     2761 ctcaggactt tcttaaagta gtaaaagccc atgcagaaga gggcgttgac tttatgacta
     2821 tccacgcagg tatcaacaga cgtgcaatcg aagctttcag acgtgaggga agaaaaatga
     2881 atatcgtttc ccgtggcggc tcactgctct tcgcatggat gatgatgaca ggcaatgaga
     2941 acccattctt cgagtattat gacgaggtgc ttgaaatact cagagagtat gacgttacta
     3001 tctcacttgg cgacgcactc cgcccgggat gtcttgcaga cgcctccgac gccgctcaga
     3061 tctccgaact tatcgagctt ggcaacctca caaagagagc gtgggaaaag gacgttcagg
     3121 taatggtaga gggtccgggt catatggcaa tgaacgagat cgccgcaaat atgataatgg
     3181 aaaagaagat atgccatggc gcacctttct atgtccttgg acctctggta acagatatcg
     3241 cccctggcta tgaccatatc acatcagcta tcggcggagc tatcgccgca gcaagcggtg
     3301 cagacttcct ctgctatgtt accccagccg agcacctcag acttcctgac gttgacgacg
     3361 ttaaggaggg cattatcgcc agcaagatag cagcccatgc cgcagatatc gcaaagggcg
     3421 taaagggtgc aagagatatc gacgataaaa tggcagcggc acgtcacaaa atggattggg
     3481 acgaaatgtt cgagatagca cttgacggtg aaaaggcaag aagattcttt gagcagaccc
     3541 cacctgccga cagacatacc tgctccatgt gcggcaagat gtgtgccgtt agaacaacaa
     3601 acatgatact cgagggcaaa gaggtaaaat tctgctcaga gaagtagaaa actcgatgta
     3661 ggggcgaaca gcgttcgccc gcatgatata aaaattgaat gtcagctcga caattgtacg
     3721 aaaaattgga caattgcatg agagcagata taaaaagaat gtaatgtgca agaggagcgt
     3781 cttgcgaact ttttcataaa aagtattcag cgagggaacc cgtgtttcgg ggtgagaggg
     3841 tgcaatggag caccgaccga aaataattta cgtcagatcg tgagcggata gcatagccac
     3901 ggtctgtgcg ccgctggata acttgctctt tttcgttcgg cggtgcgata cgatatggag
     3961 gaataaaaat gaaaaagaca aacattcaca aattggtatt cgcagcagta ttggtagccc
     4021 ttgcagtggt aggctcgctt gtttcgttcc ctatcggtgc ttcaaagtgc gcacctgttc
     4081 agcaccttgt aaatataatc ggtgcagttt tccttggacc tgcatgggca gtgggcgttg
     4141 gcttcacagc cgcacttctc agaaatcttc tgggacttgg ctctctcctt gcgttcccag
     4201 gctcaatggt tggcgcattc gttgcaggta tgctttataa gtacataaag aagctccctg
     4261 ccgcttatgt cggtgagctt ttcggcacat cagtcctcgg tggacttctt gcataccctg
     4321 ttgcaagatt tctcgttggc gcaccagccg cagcaataac agtttatatc ctgcctttct
     4381 ttgtaagctg tgcaggcggt acagttcttg ctatcgttat cgttacagct cttaaaaagg
     4441 caaaagtatt tgataatttc cttggcttct caaaggaggt aagagcataa tgggctctat
     4501 aattgaaagc tgtgcgaaag cagcagacaa ggtaagagat atatgccctt tggtgcattg
     4561 tatcacaaat tatgttactg taaatgacgt tgcaaactgc attctcgcca ttggcgcttc
     4621 acctatcatg gcagacgata ttgcagaagc agcagacata acttctatct caaaggctct
     4681 cgttataaac atgggtactc tcaatgcaag aacggtcgag tcaatggtag ctgctggaaa
     4741 gaaagccaac gagcttggcg ttcctgtggt ttttgaccca gttggagcag gcgcatcaaa
     4801 tttcagaaat gaaactgcaa agcgtatact tgaaaatgtg aagataagca tactcagagg
     4861 aaatctttct gaaatgtcat atcttgcagg actagaagtt tccacaaagg gtgttgacac
     4921 ctcggaagct gacgagaaaa acgaccctgt ttccgttgcg aaaaaaacag ccgcaaagta
     4981 taactgcgtt gcagctatca caggtgcggt ggatacaata acagacggaa aaagagtcgc
     5041 aaggatatca aacggtcacc catatctttc aaaggtaaca ggcacgggct gtatgacaac
     5101 aggacttgta ggttcattcg caggtgcttg cgacgatatg tataccgccg ctgtaacagg
     5161 cattgcaagc atgggcatag caggcgagtt cgcatatgaa aatgctggag agataggctc
     5221 aggaagcttc catatttcta tcattgacgc aatcagcaaa atgaatagtc aggtatttgc
     5281 ggctaaggct aagatagaag aagtttagta ttgcaatatt tcagatctag tggtatcata
     5341 tcaatgtgat gccactaaat tttatgtggt gatgttgctt gcaggggcga acagtgttcg
     5401 tccgtttgca ttgcatatta tacattttca tacaaaggag aatgaaaaat gaccataaga
     5461 tttgcttgtg aaaaggatat accgcaaatg aacagcttgc tctatcaggt agagagagtt
     5521 catcagcagg gcagacctga cctttttaaa gagggtgcga aaaagtatac tgataacgag
     5581 cttaaagata tgctcaagga taaaactaag cctatatttg ccgcagttga tgaaaatgat
     5641 gtgatgaagg gctacgcttt ttgtgtgttt caggagcata agggcgacaa cgttctcacg
     5701 gatataaaga ctttgtatat cgacgatctg tgcgttgatg aaaattgcag aggtcagcat
     5761 atcggctcgg tactttacga ggcagtgaag aaattcgcta aagagcaggg ctgttataat
     5821 gttactctca acgtgtggga gtgtaatcca tcggcaagaa aattttacga aaaagcaggg
     5881 ttgaagccat acaagacagg tatggaagtt attttatgat aaaagataag agggtaaaaa
     5941 atggataaat caaaaattga ctacacgctt tatctctgta cagaccgtgg acttatgtcc
     6001 tgcgaaacta tcgagcagtc ggtagaggag ggcgtaaaag gcggctgtac agttatccag
     6061 ctaagagaaa aggactgcac ttcaagagag ttctatgagc ttgcagtcag agtaaaaaag
     6121 ataacggaca aatacaatgt gccgcttatc atcaacgaca gatttgatat cgcacaggct
     6181 gttgactgtg caggcgttca tctgggacag agcgatatgc catgtgcagt ggcaagaaaa
     6241 atcctcggcg aggacaagat aataggtatt tctgcggcaa ctcttgaaga agctaaaaaa
     6301 gcacaggcag acggtgcaga ttaccttggc gttggtgcaa tgtatgccac caacacaaag
     6361 acagacgcaa gagctgtcac aaaggaacaa cttgcggaga taagagccgc tgtggatatc
     6421 cctattgtaa taataggcgg agttaatcaa aaaactgtgc cgaactttaa gggtatgggt
     6481 attgatggtg ctgcggtagt ttctgcggtg gtttctcagc ctgatattga aaaggcggca
     6541 aaggatcttc atgagctttg cgaggattgc ttcggaagat aagatgttga acgggcggtt
     6601 attaagatcg tccgtttttt gttttgaaaa atgtatgatt ttgtttttcg attttacaca
     6661 aattatacat tgtgctattg acgattaagt taaaatcggt tataataaat gaaaaagctt
     6721 tgtttttttg gattaaaacg ctaatgcgtt gaaatagtaa tgtatgttag gagagtaaaa
     6781 atgaaaaaaa ctaagatcgt atgcactatc ggaccttcca cagatgacga aaacattatg
     6841 cgtgagctta tgaaaaacgg catggacgtt gcgagattca atttctcaca ctctgaccac
     6901 agtgttcata aggctcgttt tgaaactgta tgcaagctaa gggaagaact tggcgttaat
     6961 attgcaactc ttcttgatac aaaaggacct gaggtaagac ttaaaaactt taaggatcac
     7021 aagcctgtta ctatcaagga cggcgataca ttcacgctta ccacaagaga agtggagggt
     7081 gacgaaacta tctgctctat cacatttcca cagcttccaa aggacgtttc tgtaggcact
     7141 agaatactta tcaatgacgg aaatatcgag ctgaaagcac tnnnnnnnnn ncactgagag
     7201 ttgacagcac cgatatcgtc tgcgaagtta tccacggcgg agttgtttcc gaccacaagg
     7261 gtatcaacgt tcctgacgta aacctttcta tgccttatat ctctgacgct gatatgtctg
     7321 accttgaatt tggcgcaaaa atgggatttg actatatcgc agcttcattc gttagaacag
     7381 gcgctgacgt tatttatctt agaaaattta ctcagagcct tggctggttc aatcctagaa
     7441 ttatcgctaa gatagaaaac gctcagggtg ttgccaatat tgacgagatc cttgacgctg
     7501 ctgacggaat aatggtagca agaggagata tgggtgttga gatacctttc gagcagatac
     7561 ctgctatcca gaaagagctt atcagaaaag cctacaacgc aggcaagaac gttattacag
     7621 ctactcagat gctggagtct atgataacaa acgcacgtcc tacaagagcc gagataaccg
     7681 acgttgcaaa cgctatttat gacggctcgt ctgctataat gctttcaggg gaaactgccg
     7741 caggtaaata tcctgttgag gctgtcaaga ccatgagcct tatcgcagaa acgaccgaaa
     7801 aggatatcga ctatatctca cgtttcaaga aaagagcaga cgagagaaat tcatctatca
     7861 ctgacgctat ctcacacgct actgttacaa ctgcccatga ccttaatgct gtagcgatcc
     7921 tgacagtaac aaagggcgga acaacagcaa gaacgctttc aaaattccgt ccgaactgtc
     7981 ctataatcgc acttacaact gacgatacaa catatcatca gctcagcctt tcatggggta
     8041 tcaaagcagg cgttatcgaa gagaaaacaa acactgacga actcatcaga cacgctctgg
     8101 agcgttctgt ggagcttggc tatctgaaaa aaggcgacct tgtcgttatt acagcaggag
     8161 taccgcttgg aatgagcgga acgacaaatc tgctcaaagt cgagagagcg tagtttttaa
     8221 gtgagaagtg aggagtgagg aatggcagcg ccataccgag caaatcacca acacaaagta
     8281 aaagcaccca cgagcgaagc gagcaaaagg tctgcggaga gaaccttaat aggtgcaagc
     8341 ctactaagcg gtgcagaaaa gcaatagaag aattgcaaaa aatcaattac aaagcggtgt
     8401 gggagcggaa tgctctgcac cgtttttgtt gcgaaaaatg tttgagcttt ttggagacga
     8461 tcactttttt tattcaaata attttccgtg aattgggtaa ccgattctaa catatgaaag
     8521 aagtatggcg taagagtaat atttctgaat gtgacttgac tttgattttg aagtatgtta
     8581 ttattttaac atacgagcgg tagtattatt aatagg
//
LOCUS       DAWEMR010000111         8609 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3398_length_8609_cov_10.995791, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000111 DAWEMR010000000
VERSION     DAWEMR010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8609)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3398_length_8609_cov_10.995791"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1957..1966
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2553 a         1776 c         2210 g         2060 t
ORIGIN      
        1 ggaagtaaag taacaggcgt aaagatcaag ggcataggca aatatgtgcc tgaaatggtc
       61 gcaacaaacg aggatttcac aaagatcgtt gaaacaagcg acgagtggat aactcagaga
      121 acaggcatca agcaaagaca catcacaaat ggcgagccta catactatct tggagcaatg
      181 gcggcaaagg ctgcaatggc tgacgcagga gtaaaggcag aggatatcga ccttattatt
      241 gccacaacag taacagggga ttatttcact ccgtcacttt cctgccttat ccagagagag
      301 ataggcgcga taggctgtat cgcatttgac cttaacgctg cttgtgcagg ctttgtttac
      361 ggctttgata ccgcaaagag atatctccag acaggcgacg gaataaagac cgtccttttg
      421 gtagcctctg aggagctttc aaaattcgtt gattataccg accgttcttc ctgcgtgctt
      481 ttcggtgacg gcgcagccgc tttcgtcctt gaaacagcag aggatactac atattcaagt
      541 ttccttggcg cagacggaaa cggtgcaaag tatcttgctt cccgtgcttt gaagtctgag
      601 aacgctttca gaactaatag cgaggaattt gatatggata tgcctgaaac aaaggattat
      661 ttcttcaagc aggacggcaa agaggtatat aagtttgcca caaaggcact tccaaatgca
      721 gttgagcagg cttgtgcaaa gattgaaatt tctcctgatg accttgattg gatagtgccg
      781 catcaggcaa atataagaat aatcgaaact gccgcaaagc accttggcgt ttcaatggac
      841 aagttcccgg tatatatcga ccgctatgga aacacatctt ccgcatctat ccctatcgca
      901 ttttgcgacg cagttgcaga aggcaggata aaaagaggcg acaaggtttg tcttgtaggc
      961 ttcggcggtg gacttactta tggtgcggct gttttcgagt attgataatc aatatacagt
     1021 ttatattcgg tacataataa attgttatta tgtattatgg cataaaattt gcttttgaca
     1081 ataaggagaa ctactatgac tactacaaag acaagaataa ctgaactttt gggtataaaa
     1141 taccctatct ttcagggcgg catggcttgg atagctgagc atactctcgc ttccgcagtt
     1201 tcaaacgcag gcggacttgg cataatcgca ggcggctctg cacctatcga ctacctgaga
     1261 gatcagataa gacagacaaa ggaggctgta aacggcaagc ctttcggtgt taatatcatg
     1321 ctcatgagcc ctaatgccga cgacctcgct cagctcgtta tagacgaggg cgtacctgtc
     1381 gtaacaacag gtgcaggcaa cccaggcaag tatatggctg catggaaaga agcaggcata
     1441 aaggtgatac ctgtcgttcc gtcagttgct cttgcaaaga gaatggaaag agcaggcgct
     1501 gacgctgtta tcgcagaggg aacagagtca ggtggacata tcggtgagaa cacaaccatg
     1561 tgtcttgtac cacaggtagt tgacgcagtt gagatacctg ttatcgccgc aggcggaata
     1621 gcagacggca gaggaatagc cgcttcattc atgcttggcg cagagggcgt acagctcggc
     1681 acacgtttcc ttgccgcaga ggagtgtcag ataaaccctg tttataagga gcttgttgta
     1741 aaggctaagg atacagacag tatcgtaaca ggcagatata caggtcaccc atgcagaaac
     1801 gttaagacaa agttctcaaa gaagcttgca agcggcgaag caaacggcac aatcacacct
     1861 gaggagtttg aggagataac cctcggttca ctgagaaaag ccgttcagga cggcaacctt
     1921 gaggagggtt ctttcctctg cggagctatc gcaggcnnnn nnnnnntggt aaagaaggtc
     1981 gagcctgcaa aggatatgat agaggaaatg ttcacagaag cagaaaagct tctcggcaag
     2041 taaaagacgg aggaagataa aatggcaaca gcattgataa caggttccgc aagaggaata
     2101 ggtgcagcaa tagcactcag acttgcaaag gacggctata atattgctct caacgatatc
     2161 tctgaggcaa tgtttgaaga taacgatata atggataaga taaaggcaga gggcgtagag
     2221 tgtgaaaagt tcatctgcga cgtttcaagc tatgcacagg tagaggaaac agtcaaagca
     2281 gtaaaggctc gtttcggcac tatcgacgtt ctcgtaaaca acgcaggaat cactcgtgac
     2341 ggtcttatgg ctcgtatgag cgaggagcag tacgatatgg tagttgctgt aaaccagaag
     2401 agcgtgttca atatgtcaaa atttgtgggc agcgttatga tgcgacagaa gtcaggcaag
     2461 ataataaatc ttgcttccgt tgcaggactt tacggcaacc ctggacagat aaactattcc
     2521 gcaacaaagg cagctatcgt cggcatgaca aagacagttg caaaggagct tggctcaaga
     2581 ggtatcacct gcaatgcagt tgcccccgga tttatcaaga cccctatgac agacaagctc
     2641 acagacgctc agaagcaggc tatgataaat cagatagcaa tgaagcgtta cggcgaagtt
     2701 gacgagatag caggcgttgt atccttcctt gcttccaagg acgcaagcta cgttacagga
     2761 caggtaatag aaataagcgg cggacttatg atgtagtcac tcaccgtgac ggacatctta
     2821 cgccatgtaa atttgtttta cggaatgtag atttgatatg ttttaaatga tactaaggag
     2881 tgtttaaatg agaagagttg ttatcacagg tatgggtaca gtaaaccctc ttggcaagaa
     2941 cgtagaggaa ttttgggcta atatcaaggc taacaagaac ggcctatctt atgttgacca
     3001 gtttgatacc gaaaattttc ctgtaaagat agtcggcgct gtcaaggatt ttgactgcac
     3061 tgagtatata gataaaaaag aagcaaagag aatggataga tttactcagt tcgctgtttg
     3121 ctctgcaaag caggctctta aaatggctgg ctctgatttc aaggacgttg atcctttcaa
     3181 agcaggcgtt ataatcggtg ttggtatcgg cggtcttaac atgaccgaaa aggaagtaac
     3241 aaagttcaac gaaaagcctg ataaggtatc agttttcttt atccctatga tgataggcaa
     3301 tatggcggct ggtactgttg caatgcatac aggctttaag ggcgataatt tctgtaccac
     3361 cacagcttgc gcttcaggta ctcacgctat cggcgaagct ttcagaaaaa taaaggacgg
     3421 ctatcttgac gtttgccttt gcggtggctc agaggcttgt atctctcatt tcgcactcag
     3481 cggcttcaac aatatgaagg ctctctcaaa ggcaacagag cttgataagg cttcaactcc
     3541 attcgacctg aacagacagg gctttgtcct tggcgagggt gcaggtatac tttgccttga
     3601 ggagtatgag cacgctaaag caagaggtgc aaatatcata gctgagatag caggctatgg
     3661 cgcaacaggc gacgcttatc acatgacaag cccatcacca acaggcgagg ctgccgctca
     3721 tgcaatgaag tatgcctatg aagaagcagg tctgaagcct gagcaggtga actacatcaa
     3781 cgctcacggc acatcaacag gtcttaacga taagtatgaa acaacagcta tcaagcttgc
     3841 tcttggcgaa gaagccgcaa gaaatacggt cataaactcc acaaagtcta tgacaggtca
     3901 ccttcttggt gcggcaggcg gcatggaagc tatcgttact gcactttctg tacagaacga
     3961 ctttgttcat aagacaataa actacaccac tcctgaccct gagtgtgacc ttgactactg
     4021 cgttgagggc aacagagaaa tgcctgtcta tgcagcactt tcaaactctc tgggctttgg
     4081 cggacataac gctactatct gtatcaagaa gtgcgactaa taaggagaaa ctgatatgag
     4141 cgaaaaaatt tatggtcagt ttgaccttga aacaatagag aagcttgccg atatcgtgaa
     4201 gaataaagac ctcggtgaga taacaatagc tgacggcgac aaggtgataa cagtaaaggg
     4261 caagaaatgc cctcctcctg caccaatggg aatgcctatg ccaatggctg taggcacagc
     4321 tttacccgca gcccagacag caccagaagc gtcggcaaca tcagcgccgg taacaggcgg
     4381 caaggcagtt aaagccccta tcgtgggaac attctatgcc gctccgtcac ctgattcaaa
     4441 gccgttcgta agcgttggcg acaagataaa gaagggcgac actatcttca ttatcgagtc
     4501 catgaaggta atgagcgaag tacagagcga atttgacggc gtggtaagag agatatgcgt
     4561 aaacagcggc gatgctgttg agttcgatca gaccataatg atacttgact aaactgattt
     4621 ggaggaaaga caatggcagt tgttttgaat aaagagcaga tcatggaaat aatccctcac
     4681 cgtgaccctt ttctgcttat cgacgaggta aacgagcttg aagtgggcaa gcgtgtaaag
     4741 gctacaaagt atataaaggc tgaggacttt tggttcaagg gacatttccc gaactatcct
     4801 gttaccccgg gtgtacttat ggtagagatg tgtgcgcagg caggtgcagt tgcactgctt
     4861 tcacttcctg agaacaaggg caagataggt ctgttcggcg gcataaacaa ctgtaagttc
     4921 cgtcagcagg tagtgcctgg tgataagctg gatatcgagg tggagataat caaagtcaag
     4981 ggtcctatcg gcgttggaaa ggcacttgct tctgtaaacg gcaagaaggc tgtttccgct
     5041 gagataactt ttgcgataaa gtaaggtatc tcttattcta gtcataatta gtgaggcata
     5101 atatgttcaa aaaagtattg atagcgaaca gaggcgagat cgctgtccgc attataagag
     5161 cctgccgaga gcttggcata catactgttg cggtatattc aaccgccgac agaaacgctc
     5221 tccacgctca gatagcagac gaagcagtct gcataggtcc tgcaccaagc aaggacagtt
     5281 atcttaatgt cagggcgata atctccgcct gcgagataac aggtgcagac gctatccacc
     5341 cgggctttgg ctttctgtct gaaaatccgt cctttgcgag aatgtgcgaa aagtgcggca
     5401 taactttcat aggaccaaga gccgagtcta ttgaaatgct tggcgataaa gcgcaggcaa
     5461 aggaaacaat gaaagaagca ggcgtgcctg ttataatggg ttcagacggc gctatctcaa
     5521 atatccatgc ggcgcatacc cttgcaagag atcttggcta ccctgttatg gtaaaggctt
     5581 cagcaggcgg cggaggacgc ggtataagga tcgttcactc cgacgatgag cttgaagcgc
     5641 agatgaccgc cgcaaagcag gaagctcttg cctgcttcgg caacgacgag atctatatcg
     5701 agaaattcat agaagaccct aagcatatcg agatacagat acttgcagat aattacggcg
     5761 acgttatcta tctgggcgag cgtgactgct ccatgcagag aagaaatcag aaggtcctcg
     5821 aggaagcccc ttcccctgca agctttatga atgaggagct tcgtgcgaaa atgggcaaag
     5881 ccgcttgtac agccgctaag gtctgcggtt atagaaatgc aggcactatt gagttcctcg
     5941 tggataaaaa cggcgagttc tatttcatgg aaatgaacac ccgtatacag gtggaacacc
     6001 ctatcacaga ggctgtcaca ggcgttgacc ttgtaaagca acagctcctt atcgcatcag
     6061 gacagagatt gtctataaag caggaggata taaagctgac aggtcatgca atagagtgcc
     6121 gtataaatgc ggaaaacccg cttcttgatt tcagaccgtc acctggcagg ataaagtcgc
     6181 tctttatccc cggaggtcct ggaataagag tagatactgc agtttatcag ggctatgaga
     6241 taccgcctta ttacgattcc atgataggca agctcatcgt tcatggtaag aatagagaag
     6301 aagctattgc aaaaatgaca tgggcgctgt ctgagtttat cgtggatggt gttgcaacca
     6361 atgttgattt ccagcttaag cttataagac gccctgagtt tttagagggc aattacgata
     6421 acggttttct taaccgtacc gacattttgg cagatgagag gagtaaggaa taatgcttga
     6481 agatctgttt tctgtcgtta aagtaagatt taacggcgat aaggcggacg atgacaagaa
     6541 aaagaccgat atcccaaagg gtctgctttt caagtgtcca agatgcagaa acgtcacttt
     6601 tatggaggag tttcaggcaa acggcaaggt atgtcctcac tgtaactatc attcaagact
     6661 taccgcaaaa gaacgtcttg atatcaccat agataagggt agctttgagg aatttgataa
     6721 ggatatggta tcaaaaaatc ctatagattt ccctgactat gaagcaaaac agcaggcttt
     6781 gagagaaaag accggtctta aagacgccgt tatcacagga aaggctgcca taagaggcga
     6841 gaagatagtt ataatcgtta tggattccaa ctatatgatg gcttccatgg gcagcgttgt
     6901 gggcgaaaag ataacacgag ctatcgaata cgccaccgca aataggctcc ctgttatcgc
     6961 attcacagcc tcaggcggag caagaatgca ggagggtata gtttccctta tgcaaatggc
     7021 gaaaacaagc ggtgctgtgg ctagacataa ccaagcaggt ctgctttata tcgcagttat
     7081 gaccgaccct accacgggcg gtgtaacagc ttcctttgcg tcactgggcg acatcataat
     7141 cgcagagcca aaggtgctta tcggatttgc aggcagacgt gttatagagg gtactatcaa
     7201 gcagaagctt cctgacgatt tccagtctgc cgagttcatg cttgaaaacg gctttgtgga
     7261 tatgatagtt gagcgaaaga aaatgagaag agttctcgct catatactca gacttcataa
     7321 gccggcaaag gaggtgtcct caaatgagtg agctgacagc ttgtcaaaga cttgatataa
     7381 tcagacacaa gggcagaccg actgccaagg actatatccc tcttatattc gacgatttct
     7441 ttgagatgca cggagacaga ctttttggcg acgacggtgc tgttatgggc ggcatagcct
     7501 attttaaggg catacctgtt acagttatcg cagaggtaaa gggcagagat cttgcagaaa
     7561 atcagaagtg caatttcggt atgccccacc cggagggcta tagaaaggct ctcaggcttg
     7621 caaggcaggc tgagaagttc catagacctg ttatatgcct tgttgataca gcaggcgcat
     7681 tctgcggcgt agaagctgag aagcgtggtc agggcgaagc gatagccaaa aacatcatgg
     7741 agttcatgac cctcaagact cctgttatct ccataatact cggagaaggc ggctcgggag
     7801 gagcgctggc tcttgcagtg tgcgatgagc ttgcaatgct tgaaaacgct atctattcgg
     7861 taatatcacc gagaggcttt gcaagtatcc tgtggaagga ttccacaaga gagcgtgaag
     7921 cctgcgatat aatgaagata accgctgagg atcttacaag acttggcgtg tgcgaccata
     7981 taatttccga agccgacggc ggcgcacata acgacccaca gctcactgcg gagaatatct
     8041 ctgactatat cgagggagct ttgaaaagaa aattgcaaaa aggtcttgac gaattgcttg
     8101 aggggcggta taataagttt agaactgtgg gcgagtttga ggaaagttcg caggaatgag
     8161 tttttttgat gatcatgccg aatgatttgc cgacttatga gcggcggcgg ctgacataaa
     8221 tgctgcgctg agcggcagac ccagtaaagt acagaatgga ggtaaagtaa tggtatttga
     8281 taagatcaag gacattatcg tagagcagct cgacgttgaa gaggacgcag taactatgga
     8341 ggcttctatc acagaggatc tcggcgctga ctcactggac gtagttgacc ttgtaatgag
     8401 catcgaggag agctttgatg tagagatccc tgatgaagag gtcgagaaca tcaagactgt
     8461 tggcgatatc gttaagtata tcgagaacaa ggtcgaggaa taatctttga ctgtttcaaa
     8521 aagctgtgct tttgcacggc tttttttatt ttacctgatt tgtggaatat gcacaaaatg
     8581 ggagtatatt tattaaaggg tatagacaa
//
LOCUS       DAWEMR010000112         8500 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3454_length_8500_cov_9.731794, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000112 DAWEMR010000000
VERSION     DAWEMR010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3454_length_8500_cov_9.731794"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5981..5990
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2028 a         2052 c         1725 g         2685 t
ORIGIN      
        1 ttttactata aaatctttca aattatatta tatcacataa tttccgattt ggctataata
       61 tttttgatat tttctataat tttttgaaaa ctatcaaatt tatctgtttc cacccactga
      121 attccgtcaa ctcgtctaaa ccaagtaagc tgtcttttgg cataatgacg gctttcctgt
      181 ttgattttgt cgaggcattc acaaagctca gctttgcctt caaaatacgg cacgagctcc
      241 ttatagccta tcgcctgata agctgtagca ggggtaaagc tgtcataaaa aactctgcac
      301 tcctctataa gcccgttttc caccattaca tcaacacggt ggtttatacg gtcatagaga
      361 tactgtctgt cggaagctgt aagaccgata atgcaggcgt tatatggcga cttctcacga
      421 cggctgttca ccttatgctg tgaaagcggt atacctgtag tttcaaagac ttcaagtcct
      481 cgtatgaccc ttggcaggtt gttctcatgg actttctccg cagtttcggg atcgacctct
      541 ctgagccttt cccacaaaac gtgaccgccc agcgtcttgg cttcctcctc aagacgtttt
      601 ctgactgagc cgtccgtttc ggtatcgtca aagatcacgt tgttcacaag tgagcttata
      661 tatagtcctg ttcccccgca gagaatagga actttgccac ggctgtgtat ctctctgata
      721 cgctcacctg cgagcttcac ataatctgcc acggaaaaag cctgctcagg ctcgagaaaa
      781 tctacaagat aatgtggcac gccctgcatt tcctctttgg taggctttgc agtggcgatg
      841 ctcagaccct tatatatctg catagagtct gcggaaacta tctcaccgtc atatatcttt
      901 gcaagctcca ctgcgcaggc ggtctttccg ctggcggtag gtcctgcaat gacaaggaga
      961 ggtattttat tttccatagc tttcgcctcc tgcatttttt atagaactcg tctgaactgc
     1021 ttttcgatat ctcttttggt gagggttatc ataacaggtc tgccatgagg gcaatatctg
     1081 atattttcct gaccataaac ggcgttcacg agcgcctgca attctattcc gctgttggta
     1141 tcgttcgcct ttattgccgc tttgcaggcg atagaatggt agaggtcgtc aaaaagctct
     1201 atttgcggat tgagtttatt ctcgataaaa ttctttgcaa gttcgctgat tatatccgca
     1261 gggttatcgt caccaagaat gataggcacg ccctttactg caacagttgg cgcaacgtcc
     1321 ggctctatct caaagccaag ctgtgacacc ttatcgaggt ttgcggtgag agcgtcatac
     1381 tcgtcatagg aaagcaacac catgataggc tccaggaaca tttgcgtttc aagctgttcg
     1441 gcgtcgcttt ttatcttctc gaaaatatat ctctcatggg cggcatgctt atccataagt
     1501 atcatttcgt cgcccacctg tgccacaaca taggtcttga aaagctcgcc caccacaaca
     1561 ggcttcggct tttcaggctc tttctcctcc acgataacgg aaacaggctt tttctttgtg
     1621 aagctttgag cgtcgataaa cttgaaagct tctccgctct gctcctccgg ctcttcgggg
     1681 agaggtatct cgcttatcgc cttgtttatc tcctccactg tcaccttatg gggcttcggc
     1741 tcagggcctg aaagctctgt aatggctgtt tctctctttg caggctgttc aaggcttgca
     1801 agaaacatat cgtcactgcc gctgagtttt tctttctcct caggcgtaat gctcctgctc
     1861 tccacagttt taagcgttgg tgcaggcttt tgatagctgt acgaattttc gtactgcttc
     1921 tctgtctgcg gctttgtggc aggtatctca gcctttgagg cagcaggctt ttgactatta
     1981 tcctccatgg cgaatttaag ctggacgctg ttatcttttt taggtatatc aaaaagctct
     2041 ctgtcggtga aattacgctt gttttcaagg tgcatctcat taggtctgtc attcttcata
     2101 agggaatttt tcaccgcaaa gaacacagcc tcgtgcataa gcttttcgtc tgaaaaacgc
     2161 acttctattt tagtagggtg gacgttcaca tctatggtat tcggtgggat atcgatataa
     2221 agcacacaag ccgggaattt tcccgtcata agaagatttc tgtaggcttc ttcaagtgcc
     2281 gccgcacaag cccttgattt cacatatctt ttgttcacaa agaaattctg aaaacgtctg
     2341 ttaggctttg cagacagtgg cttgacggtg aagcctgtta tgtgcatacc ctgccatgta
     2401 tagtcaactt caagcatgga gtttgcaaaa tctctgccat aaactgaata aactgcggaa
     2461 tatatcttgc cgtcccctgc tgtcaatatc tcaactttat tatccctgat gaacttgaat
     2521 gatatctcag ggtgtgaaag ggtaagcttt gtaacaaggt ctgcggcaaa attgccctct
     2581 gaaacgtcct ttttcagaaa ctttagtctt gcaggaacat tatagaaaat atccctgaca
     2641 acgaaagttg tgccgtcagg acagccgcac tcctcacagg ccttttccac tgcgccctcg
     2701 ataacataat gcgtgccgta atcctcctcg ggacgtttcg taagcacgtc taccttggat
     2761 acggcacaga tagaagcaag tgcttcacct ctgaagccga gggtcataat actgtcaagg
     2821 tcatttttta ctgatatctt acttgttgca tgacgaagaa aggctatagg cagatcatct
     2881 tttgagatac ccttgccgtt atccgtcact cttatatagg tgcggccgcc gttttttatc
     2941 tcaactgtta tgacgttcgc acctgcgtct attgcgtttt caagaagctc ctttattatt
     3001 gaagcaggtc tgtctattac ctcgcctgcc gcaataagct cggaaacttc cttgctcaga
     3061 acttttattt ctgacatttt tctatccttt tctatggctc tttccaaaag ccgcatgtat
     3121 gaaaattctc cttgcctgtc ggctttgctg tcaagcggaa catgacacag ccgtcaggac
     3181 agacgatagt ttttgtatat ttgctgtcgc cgcctatctt tgtcatatcg ccgccgctgc
     3241 ggacagcttc cataatggga taaagcatca tcattgtttt actgcaaatg ccatagccgt
     3301 tcttattcac aggacagcca taggtgcaga aatatttatc ccctatctcc tcgccgtttc
     3361 ggcaatatct ttcagtatgg tcgccacgga gaaagtccgt gacctctatt tcaaactcgt
     3421 actcctcgtc ataccacttt ttcatgacgc tcacctaaac atatctcagc aagtctttca
     3481 caaaatctct aagctcgctg tcggtcattt cgtctatgtt ggtttttcta agcctgtcgg
     3541 taacgatgct gtcgcttatt ttgtcaaaag atatctggtc gctttccatt tgctcagcgg
     3601 caagtcttgc ctttttgttt tccgcttcaa gctgttcgag aagctctttg gctctattta
     3661 ttatcttgtt cggcagaccc gcaagctttg caacttctat gccgtagctt tcgtcaactc
     3721 caccgggaac tatctttcta aggaacttga tactatcccc ctgacgcttt actgcaacag
     3781 agaaatttct cacgccgtca agctcttttt caaggctgat aagctcatga tagtgtgttg
     3841 caaacagggt cttacagccc attgatctgt tggtggagat atactctgac actgcccttg
     3901 caatggagat tccgtcaaag gttgaagtac ctctgcccac ctcgtcgagg ataacaaggc
     3961 tgttcttggt ggcgtgcttt acgatatctg caacctcgct catttccacc atgaaggtag
     4021 actgacctgc ggtgagatcg tcagaagcgc ctattcttgt gaaaatctgg tcgaccacag
     4081 atatttttgc atagtctgca ggtacaaagc aacctatctg cgccataagg gtgataagag
     4141 caacctgacg catataagtt gatttacctg acatattcgg acctgttatg accgccattc
     4201 tgttgcttgt aaggtcaaga tatgtgtcgt taggaacgaa cacctcgtct ttctgcataa
     4261 gctcaacaac ggggtgtctg ccgtttttga tatcaataat gccgtcaatg gctatctcag
     4321 gctttgtata gttattgttc accgccgcag ttgcaaatga acacagcacg tctatctgag
     4381 ccactgctgt ggcagtttcc tgcacaagtc taagctgtgt ggctgcaaaa tctcttatct
     4441 ccgcaaatat ttcctgctca agagtaacta tcttatcgct tgcaccgagg atagtgttct
     4501 ctgcgacttt aagctcgtcc gtgataaaac gctcgcagtt ggcaagggtc tgttttctta
     4561 tatagttgtc aggcacaagg tcatagtaag actttgttac ctcgatataa tatccgaaaa
     4621 ctcggttata gcctattttc agatttttga tacccgtttt ttctctttca cgctccgcaa
     4681 tatcatcaat tatccccttg cctcctgaga ttatatttct cagtccgtca agctgttcgt
     4741 tgaagccgtc tttgataacg ttgccgtcct tgacattaac aggcggttcg tcaacaatgg
     4801 cgttctcaac aaggtttgag atagcttcaa gggtggatat tttattattg cagtttgcaa
     4861 gaagctttga atcaaaacct ttgagaagct cctttatttc agggagttta agtgctgtca
     4921 gcgacagtga tttaagatct ctaggggtag cggtcttata cattaccctt gtcatgagtc
     4981 tttcaaggtc atagactcct cccagtatct cttttagctc tccaagttca acaggcttca
     5041 ttgtgagctg ttcaacagcg tcaagtctgt cgattatctt tgcaggattg acaaggggct
     5101 gttcaaggaa ggattttagc atacgcttgc ccattgaagt tttggtatgg tcaagcaccc
     5161 acaggagaga gccttttttc tctttatttc tgagagtttc tgtaagctcc agatttcttc
     5221 ttgcagtgaa gccgatagtc atgatagggt cggcgtcatg ctttattatc tcggaaaatc
     5281 tgcccaccaa tgctttctgc gtatcgtgga tatatgcaaa aagtccgcac actgcaaatg
     5341 ctgcaactgt gtcaggcttt acgccgatag attcaaggct gtttgtggaa aactgtttca
     5401 gcacctcttc tgtatgtatc tcaggggaaa aatcgttctc ctccaaaagc tgaacgctgg
     5461 tcttaagctt ttctcttatg aagcttgaaa gctctttatt tgaaagaaaa gcgtcattta
     5521 taagaatttc cgcaggggaa aatctcgaca gctcatttat agccgagctt gtcatttcct
     5581 tgccctcaaa ctcaaacaga tgcacctcgc ctgtggaaat atccgcaagg cacaatgcgc
     5641 tttccttggc tctcatataa aatgcacaga tatagttgtt ggacgtttca tcaagcatac
     5701 tgctttcgat gagagtaccg ggtgtaacta ctcttatgac ctctctcggc actatgccct
     5761 tgcacatggc agggtccatg gtctgctcac agatagccac gttgtagcct ttttcaataa
     5821 gctttttcag ataaacgtcg caggcgtggt gaggcacacc gcacataggc gctctttctt
     5881 caagtccgca gtcacggcct gtcaaagtaa gttcaagctc tcttgacgca atgatagcgt
     5941 cgtcaaagaa catttcataa aaatctccca gtctgtagaa nnnnnnnnnn atatggtttt
     6001 tatatttgtt ctttacttca aaatactgct tcatcattgg tgacagcttt gaaaaatcta
     6061 tatccttggt ttccacttaa actcactccg tttcataaat ttatcatacc gcaatattct
     6121 catgctttca aaatgcggga gaacactgtt cgcccctact tgttttttcg atacgaacac
     6181 ttttggtgtc atatcgccat agaaattatc cgccccagtc cgaaggtatc ttcggattcg
     6241 ggcagacggg ggataagctc cccctgactg ttattcgttt tttgatactg tcttagtgac
     6301 agccgccgca tgatgcgcag cttcctgaac agctatgagg agcttcctct gggcaagtca
     6361 ttgggtcttc gccgtttgca gccattgaga tgatatagtt tacagagccg aggagcttat
     6421 ccataacttc tttagcttcg ttaaatgcga tcatcttagg catagccatg atctcgtcat
     6481 aaagctctct gatctgagtg tcaagtgctg tcatcttctc gccgtcctta tcaggcttct
     6541 gcatctcaac gctgagctgt gaacggagct gattgaactc gcctattttg ttctgtatct
     6601 caacatctgt atcattgata gaacaagcct tcatataagc cttgtatctc tcgtcttcct
     6661 gaatagcctg tccaagcttt cttgtcattt caattacatc catggtaatt ctcctttaaa
     6721 tattattcag ctttcgctgt tatttatctt caacaagctc gcctctcaaa gcccagttca
     6781 ttgcttttgt tatgcgaacc tgaatgaatt taccgatata ttttttatct gctttcacct
     6841 cgacgatcat atcctcgtcg ctcttgcctg tgagataatc agcaccagtt ctgccctcgc
     6901 ctgttggcag cacggttaca gttcttccca caaatcttga aagccacgct gttgacacct
     6961 ctctctgctc caaaagcagt tctctcagcc acagaccctt ctgcttgtct gagatgtgat
     7021 cctccatttc ggcagccttt gtgccgcttc gtcttgaata cacaaatgaa tagatattat
     7081 cgtactttac ctttttgata agctccttag tttcgcagaa ctcttcgtat gtttcacttg
     7141 gaaagcctac gataaggtct gttgtgaatg aaaactcagg gtctttgctt ctggcatagt
     7201 cgattatttt cagataatcc gccgtggtat agcggcgatt cataagcttt agtatcctgt
     7261 ctgagcctgc ctgcacagga agatgcaaat gcttgcacac ctttttgcat tcaagtatag
     7321 cgtcgataag ctcgtggctt gcgtcctttg ggtggcttga cataaatctt atgcggaaat
     7381 cgccatctat cttgtcaagc tctcttaaaa gctcaggaaa accatagctg tatgaattaa
     7441 cgttctgacc aagcagggtt atctccttat agccgttttt cacaagagct tttacctctg
     7501 cgataacgga ctcaggcttt ctgctgcgtt ctctgcccct gacgtaaggc actatgcaat
     7561 aggtgcagaa attgttgcag ccatacatga taggcacgaa cgctcttatc ttatcgtcac
     7621 gaagctgcaa aaagtcctcg tttatgtcaa catgattttc tgacagattg aaaatcctgt
     7681 catgctttga tattatctcc cacagcatat tatacatatc gttgtatgca aaagttccga
     7741 aaacaagatc cacctgacgg taggtctttt ttatcttctc tgcaacgtgc ttctgctgag
     7801 ccatacagcc gcagatacct ataataagct cgggattgtt ttctttgaga tgtttaaggt
     7861 cgccaagctt gccgaacacc ttgtcctcgg cattttctct taccgcacag gtattataca
     7921 atataagctg agcctcgttc acatcagatg tgaagtcata gcccaccttt gagagcatac
     7981 cttttatctt ctcaccgtcg ctgacgttct gctgacagcc gtatgaatgg ataagggcaa
     8041 gtggcttgtg accgcaagtt ctttcgtatt caccagacca ttggagcagc ttctgcggag
     8101 cggtttcctc atttattatt tccgaaaaat tctcagtcat tgatatcctc atttcatttc
     8161 gtacattctt tttaattata aatcttttca ggcttcttgt caagcgtttt gggagtgaag
     8221 agccaaacag gttactttgc aaaacaaaaa ggggcagaaa actccgcccc catagatcac
     8281 atattatccc tgagcttcgc cctgaccctc tgtatcattt tcttccttgc agtggggttc
     8341 ttcaagtcct cttgggaaaa attctactgt cgggaatgct atcttttcaa acatctcgca
     8401 tgggctgtca ctgtttgtgg aacactcctt gccaggtacg cagtaatcat atacaggcac
     8461 cattataggc acaggtcttg acaactgaat gatagcgaaa
//
LOCUS       DAWEMR010000113         8474 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3472_length_8474_cov_8.310726, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000113 DAWEMR010000000
VERSION     DAWEMR010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8474)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3472_length_8474_cov_8.310726"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3071..3080
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4208..4217
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2769 a         1515 c         2043 g         2127 t
ORIGIN      
        1 tatgccattg aaatcaccta tgacaaaaac aaattgaatc acttctttcc acattccttc
       61 ggtcggcctt ccaattctga ggtgatctgg acaattgctg aaaacgtcaa aaggggcatg
      121 ttgattcaca atatgtgtgc ggtgacttgt tcaataagcg caagttagtc gcaaatctga
      181 ggtgaaagat cagcttcttc aagcgaattc taccgattga atcaactcaa aaggaaaaac
      241 atgaccaacg atctaacctt cggtcagttc atttccgaac aaagacaaaa gcattctatg
      301 gaaagtcagg agcttgcaaa agcgcttgga atttcgggtg cctatctttc tcaattggaa
      361 cgaggtgtcc ggacgaatcc cagcacagag cttctggaca aaatcgcaat ggttttgtgt
      421 ctgaacaaaa ttgaaacgga aacgttgtac gatctgtatg caaaggtaag caggcagatc
      481 agtcctgata ttgccgtata tatttcctcc agtgaaacgg tgcagcaagc actgcggact
      541 gcccgtgatg ccaatgcaac agaggaagat tggaaacaat tcatagaaca gctgaacaaa
      601 tgaacaataa ttttgcggag caatccgcaa aattattgcg gtagttttag ctgagcagtt
      661 aaaatcgagg tgaacgaaca aatgtgaatg aaagacaaca gaaatcacaa catcaggtac
      721 gttgtccgtt ttgtaatggc aggttatttg atattgtgac agatgaaaat ttggcacagc
      781 actatgtgtc ctgcatggtt gtaatcaaat gctggaagtg ccgtcaagaa atcaaaatca
      841 tatattctga cttagtacag acaccacgta tataaaatat agagtaggag tttgacaagc
      901 agcttttgaa gctattgtcg attcctactc ttttttgttt tcggagaaac tccgttctgc
      961 tttaaaagcg ggatggagtt ttttatgccc ttttttcaag tatggcagtg cagcattgcc
     1021 aagtatccat agccttcgtt tgatttaaaa ccttatatat caaacggagg tgagaaaatg
     1081 aagaaaagca cagttcgtat ttatgataca atttcaaaag aatatgttga cattgaagtc
     1141 agtgaggaag tctatacata ttacaatagg tcacaatgga atatggatga caatgatgat
     1201 tcgttctata agcatgaaat tcagttttca gcgttaattg gcggcaatga tggtacgttt
     1261 gagaatttca gagaatttag aattgaaaac agcgttgaaa aagaggtgat aagtaagctt
     1321 ttcacggaaa agctttatga atgtctcaat ttattatcag catcagatcg tcaactgata
     1381 gagatgctgt tttttgaggg gaaaacagaa cgagaatgcg cagcatacta tggcatcagc
     1441 cagaaaaata tcaacaaaaa gaaaaggagg attctgtgcg atctgcacaa acttttagaa
     1501 agtataaaat aaagggtatc aaattcccga cttcttcccc tatacaagcg aaaggagata
     1561 aaaatcgaat ttcatgtatc ttgaaaattg aatacacagc atcgggaata catgaatcct
     1621 gcgggcggaa taaaccccgt cagccgcaga agccatacgc cacgacctca aaagagcgag
     1681 cgagaaatat gcagcttcca aatccggaat agcttttcgg aacggcgatg aaacgcagga
     1741 ggataatgct acttccgtcc agtcatagca ccccttggtc ggggaatcag gcaatgaggg
     1801 cggctccggg agatcctcgg agggatgaga tttccatgag actgatacgc tgtcagtcgt
     1861 ttccgatgtt ccgccgtgac gggtgttaag aacaaatatc acggaaaatc gtctgaggaa
     1921 tcagacgaag gcaaaacgtt aaagttatac cgcactgcct ttttgctaag acaaatcaaa
     1981 tccagcagtg cggatataac gcaagtgcag gagcattttt tcctgcattt gcgttatata
     2041 tcaaaaatgg agcgtggtaa attgaaagaa aaattaagtt gggatgaaat cagaaatctg
     2101 attttgcata gaaaaaagaa aattcattac ggtgaaaatt gccgtaaatg cgtctgggca
     2161 gatacaagat cgggaaaaat cttgtgttcc agatatcaat gcgtaaagga gagaaaatga
     2221 tgcataagta cggacaagct gaatatgcct atgggattct ccggcaaatt tcagagaaac
     2281 tgcttgccga gggaattctg acagaagaac aactgaaaca gctgaatgcg ctaaacaaag
     2341 agggctgctt tcgtcagttc tgcacagctt tggcggcgtg atttcgttgg atttggtgat
     2401 accatttttc aggaaaaagt ggtattgtgt atgtagtgta aaaatcagat tggagggagc
     2461 aaagtgaatc caaacgtaac ggtaattcag ccgacaatta cattggaaca ggagcagaaa
     2521 atccgggctg cagcatattg tagagtcagt tccgattcca cagatcaact gaattcgttc
     2581 atgacgcaga tgcggtacta tgaaaatttc cttgctgata gtaaaacgga aacattgatt
     2641 tcggtatacg ctgatgaagg cataacgggc acccgaatgg ataaacgtga ggagtttcag
     2701 cggatgctca aagactgccg cagaggtaaa attgatcgta tcatagcaaa atcaataagt
     2761 cgtttcgcac ggaataccaa ggaatgcctg accgttctgc gggaactaaa atcacttgga
     2821 attacggtac tttttgagaa ggaaaatatc gatacggcga atatttcaga tgaaatgatg
     2881 atcacgctga tgggtggtct tgcgcaggaa gagtcggtat cgatttccca gaatatgcgg
     2941 tgggcaaatc gaaaacgcat ggcagatgga agttataaaa tctcctgtcc gccctatggt
     3001 ttttccatct gcaatggacg attggaaatc aatgaaacag aagcaaaaat tgtcagaaat
     3061 attttcacat nnnnnnnnnn ggctatggaa ttacgaaaat cgcaaatata ctgaatgatt
     3121 tgaaaattcc aagcagtatc agtaaatcgg cgtggtcatt ttttagcgtg aaatatattt
     3181 tgacgaacga gagatacatt ggcgatgcgg tttttcagaa gacgtataaa acgacaactt
     3241 tgccatatat gaagaaaata aatcatggag agtatgaaaa atattatgtt tctggaatca
     3301 atgacgccat tgtggataag ttagattttg aaaaagtgca ggaattgctg gcgattcggg
     3361 gacaaaaagc acaatctaca tgctgtgaac atccgctgtc cagaaagctt atctgcggtt
     3421 tttgcggtgc aacttttaaa aggaaacgct gtcgtgaaaa atattactgg gtgtgtagaa
     3481 cgcacaatga gaatgcggaa aagtgcggcg gacagcggtt gccagaatca aaaatttatg
     3541 aaacgtttat gcgggtttac aataagctga aatcgcaata cagcgtgatt tttcctccta
     3601 taatttcaca gcttcaggag ctgaaaaacc gcaaattcag cggcaatcag cagtacatgg
     3661 agattgcaaa ggaaatcgcc aaacttaagg agcagaatca cgtacttgcc cggctgaaaa
     3721 caaaaggatt tctggacgaa gcaaagtacc ttgagcaaat tgctgaactc aattccaaga
     3781 ttaacaaact cagccgtgaa cagcataaaa tcgtgcgctc cgatggtgag gacgatatga
     3841 tagatcaaat caaggatatc gcttcaatca ttgaaaacgg catggagctg atgacagaat
     3901 tcaatgagat catgttcgaa agtctggttg agaaaatcat tgtgattaac cagaatgaac
     3961 tggaattcca tctgtacggc ggtctgaaat ttactgaaag gctggtgtaa aatacatgaa
     4021 aaacagaaca atcccatttg gctacatgat gcggaccgga gaaattcagc caaagccaac
     4081 ggaaagtaaa gccgtgcaga acatatttca ggcttactta aacggcagtt ccctgcttgc
     4141 cattgcaaat tggatgagta gtcaaggcat tccttacaat ggcatcgatt ttgtgtggaa
     4201 caaaaacnnn nnnnnnnaag tatctgggca gaaacggcta tcctgcaatc attgatgaaa
     4261 atatgttttg cagagcaaat atgcggaaga aaattaaatc gactacagtc gtcgagattt
     4321 ctgaggagct tcaggcaatc cgcagtctta catattgtgc ggaatgcggt catcggattt
     4381 caagaatcgg cggcaatacg cactctgaaa aatgggactg ccgcaatccg gaatgctcac
     4441 gattcagcca cagaattaca gatcagatgc tgattggaat aatcgtcgct gttctgaata
     4501 cagtgattgc aaatcccaat ttgcttgatg ctgatgcaga aatcagcaga tatacgccga
     4561 gtattgagat aacccgtcag cagaatgaaa tcagccgctt gatggaaact cctgatgttg
     4621 attttgaaag tgcgaaagaa gaaatatgca agcttgcaga actgaaatat gactgctgta
     4681 cctacagcaa taaaccgcag aaaacaacgc agttgcgaga agttcttgca agtcatgaac
     4741 aactgaatac gcttgacatt ggcttaccgc aatcctgtat ttcacggatt ctggtaagcc
     4801 atttttgcac cgttgaaatc gaatttatca atggcgttgt catcaaaaat atcatagaaa
     4861 ggaagatgca aaatgaccac agcgcccaat gtcagggtaa ttcctgcaaa gccgcaaact
     4921 acggaaaaca gggataaata ccatcagctt agagtggcgg catactgtcg tgtatcgacg
     4981 gaacaggagg agcagcaaaa cagctaccag gttcagatcg catattatac ggatttgatc
     5041 aatcgcaaaa aggaatggac actggctggc atatttgccg acgaaggaat ttccggcact
     5101 caagcaaaga aaagaccgga atttttgaaa atgatacggc tgtgtaaaaa gcagaaaatt
     5161 gacattgtga ttaccaagtc aatatcaaga ttcgcccgaa acactgtgga ttgcttggag
     5221 tatgtgcggc aattaaaaga ccttggaatc ggcgtcatct tcgagaagga aaacatcaac
     5281 acaatgacca tgacaagtga atttatgatt gctctgtacg gctcatttgc tcaggcggag
     5341 agtgagtcca tcagtaagaa tgtcagctgg ggcaagcaaa aagcttatgc agaaggcaag
     5401 gtggcgtttc aatattccaa gcttctggga tatcagaaag gtgcagatgg caagccggaa
     5461 attgttcctg atgaagcgga aacagtccat ttgatatacg aattatttct ggacggattg
     5521 agcttcacaa ggataaagaa tgctcttgaa tctagaggga aactgactgc catgggcaat
     5581 cagatctgga gcgaatccaa catcaaaagc attctgaaaa atgaaaaata tgtgggcgat
     5641 gtgcttctgc aaaaaacatt tactgctgac tgcattacca aaaaggttgt gaaaaataac
     5701 ggtgaacgtc ccatgtatct ggtgacaaaa catcatgcgc caatcattga tagggacacg
     5761 ttcaaccgtg ttcaacaaga acttgcaagg cgttccagta agcgtaaaat cagcgataaa
     5821 acgactaccg agcaaggaaa atattccagt aaatacgcgc tttccgaact tttgatatgt
     5881 ggcaattgcg gcacgccata tcgtcggtgt acatggacgg ctggcggtaa aaagcagatt
     5941 gtctggcgat gtatcagccg tctggatcat gggaaaaagt attgttccga atcgccgacc
     6001 attcatgagg acaagttgca tagagcgatt ctgaaagcag tgaacgaata tctcggctgt
     6061 ggagatgaga ttgctaaaat tttgaaagca aatatcggct cggttctaga gtgcaaaaac
     6121 caacaggaaa ttttgaatct ggaacagcgg ctgaaggatt tagataaagc ccgaaacgac
     6181 ttgatatcgc tgattactgg cggcggatgt gatgagaata agctggactg cgagtttgaa
     6241 aaaattcatg cagaggaaca agagataaat gaaaagcttt cggaactccg aaagaatgca
     6301 gagatttcaa cggatacgca gaataaaatc gattctgcca tggaaatgct ggcacatgaa
     6361 aactttcagg ttgaagtgtt tgacaatgtg attgtccgca agctgattga ttgcgtgaaa
     6421 gtgctgtcga aagagaaatt actgatcgtt ttcaagggcg gcgtggaagt aaaagcacag
     6481 atggaaaaat aacgaaatat caagaaaaac ggcttgcatt gcaggtcgtt ttttcatggg
     6541 agatgagaat ttgtcaggaa attttgacgg gattaaaacg caaaaatttg gcgtggaaat
     6601 tgagtgtacc ggattgacga gaaacgcagc cgcaagggca atcggcagag tatttgacag
     6661 cgtgccggag cactttggcg gcagttacga taagtatcat atttgtgata gcaaggacag
     6721 aaaatgggca gttgtttatg attccagcat tcggcgtgtg gacaaaaacg gcggtaatgc
     6781 gtcaagagag tatgccgtgg aaatcgtcac accggtgctg gaatatagcg atattccact
     6841 gttgcaggaa attgtccggg cggtcagaag cgctgacggt gtgaccggag cacagtacaa
     6901 ttgcggtatt cacattcatg tagacggtgc accttatacg gcgcagagtt tgcgaaactt
     6961 ggtgaacatt tttgccagca aggaaaactt cttgtttgat atgcttcagg tatcgcctat
     7021 gagagaaact tactgccaga aggtggacag agattttctg gaaatgctga acaaagagaa
     7081 acctaaaact atcgaagcaa tccagaaatt gtggtataga ggcgatatgg atgaggtgca
     7141 tcatcattac agttccactc gctataaagc ttgtaattta catagttttt ttgctaatgg
     7201 gcattgggag atgagagcct gcaatagttc attgcacgcc ggagttatta gatcttatgt
     7261 gactcttgcg cttgccattt ccaatgctgc gctgacgaaa aaattctgtt cgccgcacat
     7321 ttcagagagc gataatctga ggtacagtgc aagggtttgg ctgattaatc ttggactgaa
     7381 cggtgaggaa ttccgcaatt gccgcaagca cctgatttcg catctggacg gatgtatttc
     7441 atggcggcat cccgaagatg caattgctca gcgagaacgg ctgaaagaag agcgtatcgc
     7501 agctcatgag catcctgtaa gcgaagttct ggagcaatgt gagagtttac ccgatgaagt
     7561 ggagcaagcc gctgtggacg attttgagga agattacgag caagaagaat ttgaggaaag
     7621 tatggacttt tccatatcaa tgtaaaggag ctttttatgg cgaaaaaatt atatgcagct
     7681 tacggcagca acatcaattt ggaacaaatg gcatataggt gcccgcattc aacagttgca
     7741 ggaacagcca tgctaaaggg ctatgagttg cagttcaggc atcatgccac gattgaaccg
     7801 aatgaggaaa gtgaagttcc tgtgctttta tgggagcttg attcgcagga tgaaaaattt
     7861 ttagatcgtt acgaaggcga gccgaagtat tatcgcaagg agaatatttc tcttgaactt
     7921 aacggagaaa ctgttgaagc gatggtttac atcatgaacg gagatacgcc gcttgaagct
     7981 cccacagagc agtactacgg cataataaag caaggctaca aggaaaacgg actggacaca
     8041 agatatctgg aaactgcgtt tgaagaagcg gttctagcgg aaaatatgga gtttgaatct
     8101 aaattcggaa tggagatatt ttgatgctgt atcatggatt ttatgtaaaa attacgccct
     8161 tgaaaaatat tcagagggag agaaaagacg ggagtatcag tgattgcaac ggatttcaga
     8221 ttgaaatttt ctccaaccaa tcggaagaag tgaccgtgga tgtgtttact gctgcggtag
     8281 gatttgagat actgaaaaat tctgtttgcg atgcagaaca gtttgcgatg gatgtcattg
     8341 agtcggagga aaaggaatat ttgaggttaa tcgatgaata tatattagaa aatagttgaa
     8401 attgaccatt taacgtaaaa aaatgaccat ttagtgcaaa atggttgatt tttctgattt
     8461 tttatggtac attg
//
LOCUS       DAWEMR010000114         8255 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3562_length_8255_cov_10.241829, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000114 DAWEMR010000000
VERSION     DAWEMR010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3562_length_8255_cov_10.241829"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4604..4613
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2137 a         1858 c         1697 g         2553 t
ORIGIN      
        1 cgaatgattc tagggaaaag caacaatggt tagcccaagc acaacgagat gcaagctcgt
       61 cacataaaat atttgaagaa cgttatatag agagtaagaa tgaaaaggtg caaatttctt
      121 cagatcatgc actatatacg gttgcactta cattatgtca tttagcaaaa ttggaagatt
      181 ataaaaattt gaagctaaat gaaaatgcta ttaataattt gtacttagca gtatcttctc
      241 catacaattc aatggagttc ataaagaaag ataagaatta taattacaac aatattatcg
      301 gagaaataat tacgacattt gctgcagata gatctttgat tccaagtgaa aaagcacaaa
      361 tgaatgtagt agaattaatt aaaattcaaa ttggagagaa tttttagcct aatttagtct
      421 gatgtatggg atgcatggga ttttgatttg tttccgagaa ctattgggga caacctgaca
      481 tcgaccaaaa ggtgtcaatg tagagatata cgctatttgc gagaaaaatt caaattgctt
      541 attcaatagg gttgtatggg caatttaacg aaaatgcaga cattaaaatt gaagtgtcgt
      601 tgcacgttga gtgcgtggta ttgatgtcaa ggacaacgca ggaatagcct aaaaatccgc
      661 tattctgcgt ggttttacaa tatcatcaat ttcaagtgct gcccacatat gctgtatgtc
      721 actccgtgga aacatatctg acagcaaatc taagcactat caaaatacag cactcgcagg
      781 ttgagtgctt gaaaatgttg gatttcgggt agggttgagt gcgtggaaat gttgtcacaa
      841 gaaaggaaga ttaaaatggc aagtaaaaga gttaaacaat caattatact tagtgtttcc
      901 gttatgaaag gctgttaccg ccatattcag gtttcagtga atgaagattt agaaacgctg
      961 gcagatatta ttttgtgggc atttgatttc gataatgacc acgctcacgc ttttttcatg
     1021 gacaataagg cttggagtaa ttatgattgc tactacatgg cagaagtcga tgaagatgaa
     1081 atgtaccgtc acacctgcga ttttaagctt catcagcttg gacttacaaa gggaaaagca
     1141 tttaaatttg tctttgattt tggtgatgac tgggagttca attgcaaagt ccttcgtatt
     1201 gtttatgagg atactaaaga tgcggagctt ataagagctg ttggtgaacc tcctgagcaa
     1261 tatcctgatt atgacgattt tgaagatgat gaataaataa caaaacaccc tcgccactat
     1321 cggttcactc cgacagcagc gagggtgttc gcatattttc acatattcac ttccacgtta
     1381 acacccgaat gaaattcgaa tatgatcctg tcctcgtaga ccgttgctct tttcagcata
     1441 cgtttcacaa gggagtcatc aaaggtatcg acccttgcag gctgactgtc gatgtaggtt
     1501 tcaagctcct caatccgttt tagatagtca tttctcgcat tttcgctcat tgtaagctca
     1561 tctctctgct tgcggagagc ataaatctcg cttgctatgt cggtgtagtc ctcatgctcc
     1621 tcggcttttg cgacaagctg gcgttcaaga tctgcaagtt ttgcatcaat ttctgccgta
     1681 ctttcggaat taccgcctgt cagaacgcat tcaatgttct ttttcaattg gagaagaaat
     1741 tcctgttttc cggcaagaat ctgattaagc acagctacaa aggcatcttt caaggcttct
     1801 tcatttaccg tcctagcgtt gcattctccc tttttgccga gccgtgtcgc acatcgccac
     1861 acaatggatt ttttaccacg gttattccag tgaattctgt tgaactgatc tccacattca
     1921 ccgcagacaa tcagccttgt aaagcagtga ttcgctgaaa aactgcgctt tctgcctttg
     1981 cagtcctcga ctgagccacg tcttgccatt tcctcctgca ccgcaaggaa cagctctttc
     2041 ggaatgattg cggggtgatc atcgtcaaca taatactgcg gcatgatgcc cgtattcttc
     2101 actcgctttt tctgcaaaaa gtctgtagta taggtctttt gcagcaatgc gtcacccatg
     2161 tatttttcat tttccaatat ctttttgacc gtgctgtcat accagcgtgt ctttcccctg
     2221 cccgtcagaa tgccgtcacg ctccaagccc ttggcaattt tgatacagct tacaccctca
     2281 agatattcac gaaagattcg cttgaccgtt tctgcctgtt caggatttat caccagcttt
     2341 ccgtcagcgt ctttatcata gcccagaaaa caggcgcaat tcaccataac cttcccttgc
     2401 tgaaagcgat attgcatacc gattttcacg ttttgactga gcgattccga ctcctgttgt
     2461 gcaagcgacg ccaaaattgt cagcataatt tcaccacgct ggtcggtgga ttcaatgccc
     2521 tctttctcga aaaatacagg gatattcttg tccttcagct tgcggacaaa attcaaacaa
     2581 tccacggtat ttcgggcaaa acgtgatatg ctttttgtga caacatagtc aattttgccg
     2641 tccatacaat cctgaatcat tttattgaat tgctcacggt atttggtcga aactgctgat
     2701 ataccgtcat cggcatatat gcctgcaaat tcccatgcag ggttattcgt gatgtagtca
     2761 taataatgct catactatgc ctcgaaactg gtctgctgtt cctcgttttc cgtagaaact
     2821 ctgacatagg cggcaacacg cagttttttg accgtttctg tatgccgtgc ggcgttgttg
     2881 ccgatctgcg gttttggtgg gattgttcta accatttatt tcactcctat cagactgtat
     2941 aaatattctg cctgctttgt aaaattttct tgcggttctc tttcatcaga aagcacaaaa
     3001 tttgtgcgta ttaccggcac ttttcgtctt gtctttcccg tagatttctg acgtttttgc
     3061 agttctatct gaactcgacg gaataaattt tcatccacga ttcgaggata aaagctgtca
     3121 ccgatgtaac attttctctg taaaagctgc tttaccatac tgtgggtaat tggatgtccg
     3181 gctgtttttg cggattctgt caaactcatg cccgatacat aattctgaaa aatctgacgg
     3241 accttatcag cggcttcttc tacaataaaa atttttccgt cttttacttc gtagccgtag
     3301 ggaatgtggc tcatagcagt gcctccttta acgtcagtcc gcatttcagc tgaatgccga
     3361 tgctgtggcg atcatatacg attattttct caataaaatc cgttgcaaaa aagtccttaa
     3421 actccgtctg catttctgca ctgtcaagga aatgcaagag tttctcggtt tctgcaacca
     3481 tttcggaatt tccagtatca atctgactca actgcgtgat ctgcctgcga agctcctctg
     3541 aacgcttgtt acttgcgttc tgatcctgaa taaacattgc aggcttgatg attttatctg
     3601 ccaagagatg ccttagaacg tcaattttct cggcttcttt gatgagttct gcctttaaaa
     3661 gtttgatttc ttccgaattg gcattgcatc ttttcagctc tgccagataa ggaaaaagta
     3721 tcagttttct gccaaaaatc agcttgttca tcatcgtagt gatcgttgct ttcagtgcat
     3781 catcacgcac atatttcata gagcatttct gaatgtcatc aatatgtgtg gtacaagccc
     3841 atgcgatttc agaaccgtgg tcgtggattc tccgcttgaa agttccgcca cattctccgc
     3901 agacgataat tcccgaaaac gggtatctta cttgatattt gctgtgtcct ttgacaatgc
     3961 ccttttcagc agctcgctgc tctaaaagtc ggtttgcagt ttcaaaaatc tcacggctta
     4021 ttatagcctc gtgatgattc tcaatgaaga tttgttttga cgaatttttg gtttcatgtc
     4081 tgcggaaact gccgtctgtg taggttttct gaaaaagagc gtcacccgtg tacttctcat
     4141 ttttcagcat atcacgaacg gtggaagaac tccattttcc gccttttctt gtaggcacgt
     4201 ttctttcacg gagcagcttg caaatggaat gtgtgctgtg tccgtttatg gcgagattga
     4261 aaatccagcg gacaatttca gcttcctgtt tatcgggtat catttcacca ttttcgtttt
     4321 tcacatatcc atagggtaaa taggattgct tgtatgtacc gtcctgaaaa cgctttcgga
     4381 tagcccattt ttcattttcg gaaatcgaaa tagactcctc cgccgcaaaa ctgctgaaaa
     4441 tggaaagcat caactcgctg tccatatttc ctgtgtctat atgctctttc tcgaagtaaa
     4501 tgaaaatgcc tttgtctttc aaattacgtg cgatttgcag acaatccacg gtatttcggg
     4561 caaaacgact gactgatttc gtcaaaatat aattgatttt tccnnnnnnn nnnttcctgc
     4621 attgcaatca cggagcatat tctgcaaacc gtttctcgtt tcagattttg ttccggaaac
     4681 gccttcgtca aagtaaagtc ccgcaaactc ccaagttgaa tgggctttga tgtacgtttc
     4741 gtagtggttt ttctgcgcgt caaggctctc tttctggtca aggttatcgg tcgatactct
     4801 cgcgtaggca gccacttttt tcttaggtgg attcggctgc ggtcttgcgt caatttttgt
     4861 aacagccatt gactttccct cctttcggta tgaacatatt aaactaaatt tggcgatata
     4921 tcaagtcttt tcggcaataa gtcagcgtaa agcggagaga aagattggcg gtttaatgcg
     4981 gttaatttgt cgtattcgtc aaaggaaatc agtccttttt caagcatttt ttcggtgatt
     5041 ttctgcgcca taaaatagcc gagttcatcg tgattttctt tcattaaaat gccccccatt
     5101 catacttcgg aaaaaagtca gctcttgtga taaaaattat cccggagcag ttttaacaac
     5161 tccgggatag aaaaaatttt gagataatca gatgtcagat ttatttaccc aaccagtcac
     5221 atacttgcca atgggtgtat tgccgcaaaa ctccggctta gttgtcacac gcattctgcc
     5281 gttgacaacg atgccgtcgt aaagataata tgtgccggaa cgcttcacac ctgtttttgc
     5341 agctgcggat gcgtaaagtg cggtattttt gagtgttacc tttgcacctt ttgcatagga
     5401 ctttccattg ctgaaaacaa cattcccatt tgcatcaaag acagaatatc ccgtcttgca
     5461 ggcttttttt gcattttcca atgaggaata tgccccaatc tgcgacttag catcagccca
     5521 tgtttttctt actcgataaa gctgttttgc aggtgtagga gttgccggtg tagaaccaga
     5581 attaagataa gattgtacct tagctttaaa agctacccaa tgaggcagaa tgtacgcagg
     5641 acacatcttg taaggatttc ttgcagtatt gagatagtcc acagagccac tctttccatc
     5701 acagacattg agccaatgcg tatgcgtgta aaggtgattg atgtcaagac catacttctt
     5761 cagaagcgca gcggcaagtc ttgcacaatt gtcctcagac ttcttatctg tcacattata
     5821 cgcagatgac ataatgcact caattgcgat tgtcctgcga ttgccgttgc cactaccatc
     5881 agcggcgtgc cagccgctca gactgtgggg cagattctgc catgcacagg tattatcaac
     5941 gtaataatgc accctgacat ctttcatatt gccattaaca gttgctcttg tatactgctc
     6001 cgcaggtgtc gttccgcttg ccacagaaat ccagtcggta ttgtgaactg ttacaccgat
     6061 aactttgcct tccattgaaa cagagggcat atcgatgtgg ttgggattgt gtttggtgag
     6121 taaatactcg ttgattttta ctccgttcag agtcgttgtt gcatctggtc ttaaaatagt
     6181 catattactt gtcctccttg tttgattttt cttcggttct gccgactttc gtctgcaaaa
     6241 catcgattgc ctttttgatt gcaggcgggt aggggatacc cattaagctt gtattttcaa
     6301 tgatggataa cagttcgttc aaacaaaaac tgatgcaaac ggcatcccgg atgtagttgg
     6361 tattcagcag aatatccatc cgaactgcaa cgacgatcag catcaaggtg cagacttttt
     6421 tcgccagacc gaaccagccg gctttggaag aaagtccgcc gctttccgtg tgtttggatt
     6481 ttttcatcat ggcggtgatg atgccggtga agaagtcgat tgccataaag acgaccagtg
     6541 tcaccagagc ggagtcccag ccgccaaaaa tggcagtaaa aaagccgccg accaagccga
     6601 cagctacaca aatggtatct ttcattttca accctccagt actttcagga atcggatttt
     6661 cggatgagaa ttgttgcttc tgcccaccca ggcaaggtaa tattcgccgt cagaaatgcc
     6721 agtgcattct gtgatggtgg tgataaaggt atccgactgc agccattgga aatccagaga
     6781 aaccgcacga tttgcatcga tctctgtgtt cacatacaca ccaataggaa tgtcgatctt
     6841 ctgcggtttc tgcaccagat acagacttcc ggcttcgctg gaacccgact gataggacat
     6901 cacgatttcc gcatttttcg tcagagataa gggctttgca caaacggtca agaccgactt
     6961 atcccagtta aaacactcct gcgaatagga cagcacgaag tcattttctg cactgcaaaa
     7021 ctgcggatag gcagccagaa aatctgtcat tgtctgatat ccgccgtcca gaatcatgct
     7081 gatgtccggt gcataggtcg aaacggcatt ctgtccggac tgaaacagaa tggtgtaatt
     7141 tctgccgctt gtcaggttgt cgatttgctt ttggagactt tccaaagtgc gttctgtctt
     7201 ttccgaatag actgtaacct ttgtactaag cccattgatt tgtgtgccaa aaccatccca
     7261 ttgtgcgatt ttagtggcag tgatctgatc caatacggct tgattttcgt gggtatgtgc
     7321 cttttccaaa agcggctgaa ccgcatcctg aatcagtact tttacggcat cggtatccgg
     7381 ataatttgtc aaatccggag aaacaccatc ctttccgtta cgcccatctt taccgtcttt
     7441 cccatttgtg ccatccttac cgggcaaacc gtctgcacca tcttttccgg gtaatccatc
     7501 cctgcctttc aaactctcca gccattctgc aactgtcccc acaaaaccgt tttctatggc
     7561 aatttcataa gcagaacgac cgtcttttcc gtttgttccg gtttgcatct ccgaaagctt
     7621 tttcaaaagc tgcgtatata aatccggcgt cggcggaatt ggcatatccc catctgcaac
     7681 aaaaccagac ggtcgaatat gcagtgtgac gggtaccgtg gtcgcacgca gtgtagtatc
     7741 gctttctgca tcgtagccaa acaaactcat cttcactgta ccgggatgca gttcggcagg
     7801 cagcaagcag gtcgttccct ctgtgccaag caccaagttg tatgtttctt cgcactgcgt
     7861 gaactgcacc accttgtgca gcgttttcca agccccatcg aatacgaact tcaccgaaac
     7921 aaatgcgatc tggtcagagg caagaacctc tcgctccagt gcttcgattt tttgctgttt
     7981 cactaaaaat ttcatcatcc gtttttcacc tcgttccaca cattattttc aggatcatat
     8041 tccaaatagc cgtctgtaca ctgaactttt tgcagatagt cattgtaaaa atgctttccg
     8101 gaggacatcc agttggtcgg tttggtgatg gcgttccact gagcgatcgt tccttcatat
     8161 gtgatggctg ttagactttc acagtatgtc agcatatttt ccccaaaggt tctgcaattc
     8221 gcagaaatga taaggctaga caatgctgta catct
//
LOCUS       DAWEMR010000115         8205 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3584_length_8205_cov_11.550675, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000115 DAWEMR010000000
VERSION     DAWEMR010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3584_length_8205_cov_11.550675"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7319..7328
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2081 a         1800 c         1639 g         2675 t
ORIGIN      
        1 caagacctcg ataaaatcgg ggtcttgttt ttttgcataa aaataaggct ctgagttttt
       61 gctcagagcc ttgctttgtt atatttagct gtccataccc ttacgaacag acttgataaa
      121 cttgccaaca tattcaggag cgtctttgcc gtgttcctca actatcttga caacagcact
      181 tcccatgata acgccgtcag cgtactggga cattttcttt gcctgctcag gatttgaaat
      241 tccgaaacct atgcagcaag ggcagtgatc gttgtttcgg ataggtgcaa gcagctcgtt
      301 aaaatcagtg gttatctctg aacgcatacc tgtaacgccc agtgaagata caacatataa
      361 aaagcccttt gacttttctg ctatcatttt tacacgctcg tgtgaggtcg gtgcaacgag
      421 gaagatagtg tagataccat ttttctcagc ctcgccctca agctcttcac gttcttcaaa
      481 aggcatatct ggaacgataa cgccgtctat ctgtgtttct ttgcaaagct caaagaactt
      541 tgctgtaccg aacttgaaga tagtgtttat atacatcatc agaagcatag gcttgtcagt
      601 ctgtgtgcgg agttttctta caaggtcaaa tatcttgtca agatttgttc caccactgag
      661 tgaacgcaga gaagctttct gaatagtcgc accctctgcg atagggtcag agaacggcac
      721 tccgatctcg ataatatcag aaccattctt atacatttca agaacgcact tttctgttgt
      781 atccatatca gggtcacctg cggtgataaa tgtcacaagc gcctttttgt tttctgtttt
      841 aagcttttca aagcaagcgt ctatcctatt cttcattgag ctcgacctcc ctgtatcttg
      901 ctattgagta tacgtccttg tcgcctctgc ctgaaaggtt gatgacaagt attgagtcct
      961 tatccattgt aggagcaatc ttaagcgcag cggcaacagc gtgtgatgac tcgatagcag
     1021 ggattatacc ctctgttctt gaaaggtact caaaagcaca aactgcttct tcatcggtta
     1081 tgtcaacata ctgcgctctg ccgatagagt taagatatgc gtgttcaggt ccgatacctg
     1141 ggtagtcaag acctgcggaa attgagtaaa cagggtcgat ctgaccatat tcgttctgca
     1201 agaaaagcga tttcatgccg tggaatatac caagtgaacc ttttgcaact gtagccgcat
     1261 ggtcaggtgt gtcaatgcct ctgcctgctg cttctgcacc aacaagcttg actgacttgt
     1321 cttcgataaa gttgtagaat gcacccatag cgtttgagcc gccgcctacg caggcaacaa
     1381 cgcaatcagg tagtctgcct tccttttcca taagctgtga ttttatttca gcactgatga
     1441 ctttctggaa atctcttacc attgtaggat aagggtgagg tcccattaca gagcctatgc
     1501 agtagtaagt ggtgtcgata tttgataccc aatctctgaa agcctcattg atagcgtctt
     1561 tcagtgtagc agtaccgctt gcaacgcctg ttacctttgt gccaagaaga ttcattctgt
     1621 aaacgttaag ggattgtctt ttcatatcgg tctcgcccat gtagacctca cattccagac
     1681 ccataagagc gcaaactgta gcggtagcaa caccgtgctg acctgcgccg gtctcagcga
     1741 tgatacgttt tttacccatt ttctttgcca gaagtatctg acccaaaacg ttgtttatct
     1801 tgtgtgagcc tgtgtggtta agatcctcac gtttgatgta tattttagct ccgccaagat
     1861 ctttggtcat cttttcggca taataaagca ttgacggtct gcctgcataa ttgtggtaaa
     1921 gctcctggag ctctttgttg aactcaggat cgtccttgta tttatcgtaa gctttttcaa
     1981 gctcgtgaac agcagtcatg acagtttcag gaatatattg tccgccaaat tcgccaaatc
     2041 gtccaatttt actcatttat atccttcttt cggtattatt tctgatagtt tcaactatcc
     2101 tttttatctt gttttcgtcc ttgaagccat cggtttccac tgaacttgaa aagtcaacgc
     2161 cataaggttg taccttttcc atagcatagc agatgttatc ctcgttcagt ccacctgcaa
     2221 gtaagaaagg ctttgaaata ctcacattgg agaacacatc aaggtcaaca cgcttgcctg
     2281 ttccgccgta gctgcctgct gaaaagctgt ctataagcag tttgtggacg ggaagtgcgt
     2341 cagcttttgc aatatcatca gcacatttca tacgaacggc tttccatatc tcacagtcgc
     2401 agttttcacg gagcttatca atatactctg cgttttcctc accatgaagc tggataacat
     2461 caagcttctg tgcaaagctt ttcagtatat tctctatagg ctcgtttaca aaaacgccca
     2521 ctcgtttgat ttccttatta agaagaagtt caagatcgta gaatgtgtca atgtctaccg
     2581 tgcgtgtaaa gccctccgac agtataaagc cagcataatc agggtggtat gtgttcacat
     2641 aaagaatatc gtccacacgg cgcataccac atatcttgat ttttgtcatt tatacgccct
     2701 ctctcagttc atggagagtt gtagcgatat tgccgcttct cataagcgtt tcacctataa
     2761 gcacagcgtc tgcaccaagg ctgcgtacct ttttcatatc ggcatttgtt tttataccgc
     2821 tttcagaaac gagcactgca tggtcgccta ctatattgct gagcctgccc gtggtttcaa
     2881 ggtcaacgtc aaaagtgtaa agatttctgt tgtttatgcc aagcatatca ggcttaaaac
     2941 caagcgtctt tttatactct tcctcgttgt gcacctctac aagacattca aggccaagct
     3001 cgtgtgcaat gtcagaatat tctttcatct gaccctcgct gagtatagcc gagataagca
     3061 gcactgcatc agcacctata gcccttgcct cgtaaagctg atattcgtcg ataacgaagt
     3121 ccttgcggag tatcggcagc tttacagccg ctcttatagc tttgaggtat tccccagagc
     3181 cttgaaaata gtgctcttca gtgagacagg aaatagcatt cgcaccgttt ttctcatact
     3241 cgcaggcgat tttcactggg tcaaaatcag ggcagataag tccctttgac ggcgaggctt
     3301 tctttacctc gcttatgacg gaaagggtgt cacgcttgat agattttgaa aaggaaactg
     3361 tctgatactc tttttcggct gccatgtatt tgatatcctg tcttgaaact tttgatatat
     3421 ctctttcaag ctgttctttt cttttgtcgc agatattgtc aagtatcatt ttttatcctc
     3481 tgttagtaag ttcaacaagt tcctcaagct ttttaagagc ttttccgctg tctatcattt
     3541 cagctgcagt ctttacgccc tcttcgatgc tgtcgcattt gccgcagata taaagtgcac
     3601 agcctgaatt gagaagaacg atatctctct ttgcaccctt gatagtgccc tcgagtatac
     3661 ctcttgtgat agctgcgttg ccctttccgt cctcgccaac gatatcagca agctttcctc
     3721 ttttaagacc gaagtcctca ggctttatct catacttgat aagctttcca ccatttatct
     3781 cgcaaatagt tgtaggagct gatatagaaa cttcgtcaag tctgtcgttg ccgtaaacga
     3841 gcattgctct ctttatgcca agattcataa gcactttagc cattggttcg agtagatcag
     3901 gatcataagt gccgagtatc atgaagtctg cttttgcagg attagcgaga ggtccgagaa
     3961 tgttgaaaac agttcttaca cctatctgcg ctcttgtagg tcctacaaat ctcattgctg
     4021 agtgatatga ctgtgcaaac aggaatgaaa gaccgatctc gtcaagggac ttttcagcct
     4081 gctcatgtgt tgactgaatt ttaactccaa gagcctcgag aacatcagcc gcaccgctct
     4141 ttgaagaaac gcttctgttg ccgtgttttg cgactttctg tcctgctgcc gcaattacga
     4201 atgaagaggt tgtggaaatg ttgaagctgt ttgaaagatc tccacctgtg ccaactatat
     4261 caagagtacc cttgacattg acctttgaca ttttttctct cattaccttt gcaaagcctg
     4321 ttatctcgtc gatagtttcg cccttcattc tcagtgccgt gagcaggcag gcgatctgag
     4381 cattagaagc tctgtcgctc atgatgatat ccattgcttt ataagattcg tcctcagtta
     4441 gtgactttcc atcgcatact attttgagat actttttaag ctcaactctt tcgctgtcag
     4501 gaagagctgc agactttgtt tttgtggtct ttatgccagc gatatcgttc aggaagtttt
     4561 cgattatcat gtttccaact tcgccagtga gaactgactc agggtggaac tgaacgccgt
     4621 aggtcgggtg ttctctgtgt cttattgcca ttatctgagc tttatcgtca gttgcaataa
     4681 cagacagaca ggtaggaagt gaaatgctgt caacaacaag tgagtgatat cttgcggctt
     4741 ttattgtgct tttcagaccg ctgaaaagtg ggtttgcagt gttaagattg atatctgttt
     4801 gcttgccgtg aagctgctgc tttgcgtgaa ctatctttcc accgaatgct tcggctatcg
     4861 cctggtgtcc tagacaaaca ccaagtatag ggatcctgcc actgaatgtc tttatgacct
     4921 cttcggaaat tcctgcgtcc tttggataac caggaccagg ggagatgatg atagccgcag
     4981 gtgacatttt ttctatctca tcaatggtta tctcatcatt tcttgctact gtaatgtctt
     5041 ttgtgagtac gcctacagcc tgataaagat tgtaggtaaa gctgtcatag ttgtcaataa
     5101 gtaaaatcat agtaatcttc ctttacagta caaacttatc aatgaaagtt ttgtcgttga
     5161 agtagtttat gccgatagca ttccttattt ctgaaacagt ttcgtttgaa acctctattg
     5221 cacgttgtgt gcctttttta agtatctcta tgagctgctc tttgtctttc tcatattcct
     5281 cacgtcttgc tctgataggt gcgagaaatt cttgcataac gttgttaagg aacttcttac
     5341 atttcatatc gccaaggccg ccacgctcat aatgttcttt catctctgcg aggttcttat
     5401 agtctgggaa gtatttagca aaatcttcgt ccttgctgaa tgcatcaaga tagataaata
     5461 ccggattgcc ctctgtgtga ccagggtcgc ttacattgat gtgtgtaggg tcagtgtaca
     5521 ttgacattat cttgtttttc agcgtttttt catcgtcttt aaggtaaatg cagttgccaa
     5581 gagatttgct catctttgtg ttgccgtctg taccaacaag tctgttgcag cttgcgttgt
     5641 ctgggagaac agcctcaggc tcaacaagta tatcgcactt ataaattctg ttgaagctgt
     5701 ttactatctc tcttgcctgt tcaagcattg gaagctgatc ttctccaaca ggaacatact
     5761 tagccttgaa tgctgtgatg tctgctgcct gactgatagg atatgtcata aagccaacag
     5821 gaaggtttct ttcaaagttt ctcattttta tctcagactt gatagtaggg tttctttcaa
     5881 gtcttgacat tgtaacgagg tttgaatagt acattggaag ctcataaaga gctgggatat
     5941 gtgactgaac gaagaatgtt gtcttgtcag gctcaagccc taccgcaaga tagtcaagca
     6001 ttacctcaag aatgttttgt cttattttct caggattgtc agcgttatct gtaagtgcct
     6061 gaagatcggc tatcataacg aactgatcaa attttcctgt gttttgaagt tcaactcttt
     6121 ttctgagaga acctacataa tgaccaagat gaagtgatcc agttggtctg tcgcctgtaa
     6181 gaataatatt attgctcatg ttattaactc cttttaaatt ataatttgct tgcttctttt
     6241 attgcattta taacagccat tgccttgttg catgactcct gatattcgtt ctcaggaact
     6301 gagtctgcga caactccgcc tcctgcttgg atataagcct tgttattttt gaaaagcact
     6361 gttcttatag cgatacaagt atccagcgaa ccgtcaaatg caagataacc aactgttcca
     6421 ccataaaggc ctctttttgt tgtttcaagc tcgtcgatta tctccattgc tcttaccttc
     6481 ggtgcgcctg aaagagtgcc agcaggaaga actgccataa gtgcgtcaag aggtttcttg
     6541 tcttctctca gtgtaccctt tacattcgat acaaggtgca tcacctttga atatctttca
     6601 actatcatca tatctgtgac agttacggag ccgaactcag aaacctttcc tatgtcgttt
     6661 cttccaaggt caacgagcat tgtgtgttca gctctttcct ttggatcgtt gagaagctgt
     6721 tgttcattgc ggatatcttc gtcgtaggtc gttccacgct tgatagtgcc tgcaattggt
     6781 cttgttacta ctgttctgtt ttctactgat acaagcattt caggtgaagc gcctgcaaca
     6841 gcataatcat tgtgcttaaa gtagtaaagg taaggtgacg gattagttga gcggagcatt
     6901 ctgtaaacgt caaagctgtc aggcggattg tctatctcaa atctctgtga cggaactatc
     6961 tggaagatgt cgccctcttt gatgtattcc ttagcctttt caacgtttgc gagataatgt
     7021 tcctttgtga tgttggattt tactgtgatg ttgtcctcca ttgtctttgg agttttcata
     7081 acaggcttgt aatttctaag agtttcaact acttcgttgc atatcttttc acattctttg
     7141 tatttagttt caagatcatc gctcttattg atgttgagga ttattacagc cttgtttgaa
     7201 aggtgatcgt aagcaacgat cttttcaaaa aggtgcaggt cagcgtcagg aagcttaaga
     7261 tcgtcctcag gtgggttgca cagtgttttc tcaaagtatc ttatcatatc ataagcgann
     7321 nnnnnnnncg aaaaatccta ttaggccacc tgtgagcttt ggataattat taaatctcgg
     7381 tgacttgtgg ccttcgatta tctcagaaaa gaaagcgata ggatcatcga cttttaccgt
     7441 ttcatcagcc ttgcctttct ccttaacagt aacaacgtgc ttgtcaagaa tatattcgca
     7501 tttaggatca atgcccacat atgaatatct gccccactgg tctttattat ctacagattc
     7561 aagaatgaaa caatcatcat acgcctcgtg cagacagtta aaaagttgta ttggcgtaca
     7621 tgaatccatg agaagctcat agaatacagg cacagtcttg tagttttcaa aaagttcctt
     7681 tacttgttca atagcagggt acagcataaa tattcctcct aaatgctttt ttgtcgtaaa
     7741 attgacaata aaaaaagtcc tgccatcaca cataacatct agttatggga cgatagaacc
     7801 gtggtgccac ccaatttccc gataataaat tatcggctca cagatacgca ttctgactgg
     7861 cagaaatcaa tatcctgtcc tttaacgcag gaatcacggc gagtgatact cgaataaact
     7921 ttcacaccgc tcctcacaaa tccattcatc tgaagaaaat gtatcagctc acacctaccg
     7981 ctgactctct gtgacctttt gcatcaaact acttactttt gctcatagga tttatcgtat
     8041 taactttagt tttattatat tcctctgttt ctaaaatgtc aatatcagaa aatgtaaata
     8101 ttattgctat tatgtaaata ttcttttgcg atttggattt atactaaaat aaataatttt
     8161 aaattttcaa tattccttgc atttataaat aatgtagtgt ataat
//
LOCUS       DAWEMR010000116         8192 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3593_length_8192_cov_8.797591, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000116 DAWEMR010000000
VERSION     DAWEMR010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8192)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3593_length_8192_cov_8.797591"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2185..2194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2970..2979
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3441..3450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2588 a         1661 c         2112 g         1801 t
ORIGIN      
        1 gctcaggcgg tgcatcagaa tctgatgatt caggaacagg ttgcagctca gtctttaacg
       61 cagattgcca tagaccttaa ggaaatcaga gataggcggc tttatgcaga actcggatat
      121 tccgattttg ccgaatactg cgaaaacgcc acaaagacgg gaaaaagaca ggcttataat
      181 cttatatcgc ttgtcgagca gtacaagatt gacgatcttt ccagacttgc gtatctcggc
      241 agtaccaaac tgatcgctct taaatctctc ggcaaagagg aacgtgagga gcttatagag
      301 agcggcaaag ccgaagaact gtcagtgaga gagctcaagg aaaagataaa ggagcttacc
      361 gataaaaatg aacaattacg ctttgagttt acatcagtaa ctgacagtga caaggataaa
      421 gacagcaaaa tcaattcgtt gcaggcacag cttgacgata caggaaatgc tatgcggcga
      481 accgcagaag aaaatgaaaa gctgaagtta cagatagctg aacttgaaaa gcgtcctgtc
      541 gaagtggcag ttgccgaacc gtcggtcgag gatattgcaa aaataagagc agaagccgaa
      601 gctgccgcaa gagcggaata cgataaaaag cttgctgatg aaaagaaaaa agtgcaatcg
      661 attgcacacg aagaagcaag tggtaacggt aaagaaatct tcaagattca tctgaaaaat
      721 atacagcgtg aattcaatga agcgttagag cttgtaagca atgcgtcaga aaatgaacgc
      781 agcagttata taaaggcttt ccgtgccgca ctgaatgcgt gcgaggattt gattgcaaag
      841 ttataaggag gaaaaccaaa tgtcagtaaa aatcagttcg cttgaaattg aaaatgtcaa
      901 gcgagtaaaa gcggtacagt taacgcctgc cgagaacggt cttatgataa tcggcggtaa
      961 gaacaatcag ggcaagacat cggtacttga cgctatcgca tgggcactcg gcggtgacag
     1021 gctgaaaccg tcacaggctg tgcgggaagg ctttgtgatt ccgccccaca tggaagttac
     1081 gctcagcaac ggtataaagg tagtcaggag cggcaataac agtacgctca aggttattga
     1141 tccggacggc aacaagggcg gtcagcagct gctcaacgaa ttcgtagaac agtttgcgct
     1201 tgatcttcct aagtttctcg accggtcgag caaggaaaag gcggatactc tcctcagaat
     1261 aatcggtgta ggcgataagc tgtacgagct tgaaaccgaa gaacagaagc tgtacaatca
     1321 gcgtcacact atcggtcaga tagcggatca gaagaagaaa tacgctaagg aaatgccggt
     1381 attcgcagat gctccgaaag agtttgtgtc agcaaccgag cttatcagac agcagcagga
     1441 tattcttgca agaaacggcg aaaatcagcg taaaagacag ctcagagagc agtacgacag
     1501 agaacttgag ttggctcgga aggcatacga agaagcacag gcaagacttg aaacagcaac
     1561 ggcaaacgca gaaaccgcac atcgtgacgc tgaagacctt gcagacgaga gcacggcaga
     1621 gcttgaacag agtatagcag acattgagca gataaacgca aaggtccgtg caaatcttga
     1681 ccgtgaaaaa gctgagcttg acgctgaagc gtataaaact cagtatatac agcttaccga
     1741 agaaatacag tctgtcagaa aagctaaaac agatcttctt gacggtgcag acttaccgct
     1801 tgagggcttg tcggtagata acggcgagct tacatacaac ggttttaaat gggataatat
     1861 gtccggctcg gaacagctca aggttgcgac cgcaattgtc cgcaagctca atcctaattg
     1921 cggatttgtg cttatagaca agctggaaca gatggatacc gatacgctga acgactttgg
     1981 cagatggctt gaaagcgagg gcttacaggc aatcgccaca agagttagca cgggtgacga
     2041 gtgcagtatc ataatcgagg acggctattc aaagccggtt gaaaagaaag aaactacaac
     2101 atggaaggca ggtacatttt gatgagtaca acaatgaaca tcactaaagg cagaatcgaa
     2161 accgccaaga aggtggttat atacnnnnnn nnnncagttt cccaatccgt tattcatcga
     2221 caccgagggt agtacaaagg aaatggacgt tgcccgtttt gataaaccga catcgtggga
     2281 gttgcttaag agccagattg agtatgtcaa gctcaataag ccttgtgcta cgcttataat
     2341 tgatacgata gactgggcgg aacagctttg catcaagtct atctgcgata agtacgacaa
     2401 aaagggcatc gaggatttcg gctatggcaa cggttatgtg tacgaaaagg aagagttcgg
     2461 caggttcctt aatctgcttg aagaggttat cgaagccgga gttaacgttg tactcacagc
     2521 tcacgctatt ctcagaaagt ttgaacagcc cgatgaactc ggaagttatg atcgctggga
     2581 actgaagctc ggcaagaaaa caaccaatct gatctctcct cttgtaaaag aatgggctga
     2641 tatggtgctt ttcgcaaact acaagactat ttcggtagcg gttgacaagg acggcaaaaa
     2701 acataaggcg cagggcggca gacgcataat gtacacgtca catcatccct gctgggacgc
     2761 aaagaatcgt tacggtttgc cggaagaaat tccgatggag tacgggcaga taaagcacat
     2821 tatcgaaaga aatattgccg cacagcctgc cgctaccgtt cagactgcgc ctgttgcaaa
     2881 agtggcagcc gcagaaaacg caacaaccgc cacgaatgat aatgtaacgc cggctcctgc
     2941 tccggcaata acgcaggaaa gctcaggaan nnnnnnnnna acggaagagc agataagagc
     3001 ggcagtagca agcaagggat atttccctgc cgatatgccg ataaaggact atcccaagga
     3061 atttatcgag ggcgtgctta tcggggcatg ggagcaggta aaagcaatga taacggaaat
     3121 gcttatgaca gactatgaga acgaggctta cccgttctga taaacgaaag gagaaataac
     3181 acatgagtga atttgaaaaa gaattaggct gggacgacgt aattgagaaa gaaagcgatt
     3241 ttacgcttct tcccgcaggt gactacgact ttacgataac aggcttcgag cgtgcgagat
     3301 atgagggcgg cgaaaaactg ccgccttgca acaaggctgt agtatctatt catatagacg
     3361 ctccggaagg ctcaactaca attcagcata atctgttttt gcacagcaag tgcgagggta
     3421 tgctttcggc attctttatc nnnnnnnnnn cacggcgaac cgcttcgcat gaactggaac
     3481 aacgtcatcg gtgccaaagg tcgttgcaag gtgtacatag atacttggaa gaacaagaac
     3541 ggcgaagaaa tgcagtctaa cagaataaaa aaattctatg agccgtcacc tgcacagact
     3601 gtttctcagg cacctgcaag ctctcaggcg ggtgtattta caccgggtaa attctgatgg
     3661 aattaagacc gtatcagaaa gaagccaaaa cagcggtact ttcacagtgg gagcagggca
     3721 attcaaaaac cctgctcgta ctgcctacgg gttgcggtaa aacgatagtt tttgcaaaaa
     3781 tcgcagaaga ccgtgtccgc aacggagaaa gggtacttat acttgcgcac aggggcgaac
     3841 tgcttgaaca ggcggcggac aagatactga atgcctgcgg gcttggctgt gctgtagaaa
     3901 aggcggaaga aagctgtata ggctcatggt atcgtataac ggtaggctct gtacagtcgc
     3961 ttatgagaga aaagcgactt gcacaatttt caaaagacta tttcaatacg atcataattg
     4021 atgaagcaca tcattccatt tcggacagct atcagaagat actcggatat tttgatgaag
     4081 caaaggtact cggagttacg gcaacaccgg acagaggaga tatgaaaaat ctcggacagg
     4141 tattcgacag cctggcgtat gaatatactt tgccgagagc tataaaagaa gggtatctgt
     4201 caccgataaa ggcactcacc attcctctga aactcgatct gacaggtgtc ggtactcagg
     4261 caggagatta taaggcgagt gacattgaca cagctcttga cccttatctg tatcagatag
     4321 cggatgaaat gctgaaatat tgcaaggaac gtaaaacggt agtgtttctg ccgcttataa
     4381 aaacgagtca gaaattttgc aagatactta acgaaaaagg cttccgctct gcagaagtca
     4441 acggaaatag cattgacaga ggtactgttc ttgctgattt cgatagcggt aaatataatg
     4501 tgctgtgtaa ttcgatgctt ctgacggaag gctgggactg tccaagcgtc gattgcgtaa
     4561 tagttctcag acctactaag gtaagagggc tgtactgtca gatggtcggc agaggaacaa
     4621 ggctttgtga gggcaaaaaa gatctgttgc tccttgattt tttgtggcat accgaaaggc
     4681 acgagctgtg tcgtcctgca catttgatat gcgaaagccc ggaagtcgcc gaaaagatga
     4741 ctgaaaatat tgcaaaggca ggtatgccgg ttgacattga gcaagcggaa gaaaaagcaa
     4801 aagaagatgt agttgctcag cgtgaggaag cacttgcaaa acagcttgcg gaaatgaaaa
     4861 aacgcaagag aaaacttgta gatcctctcc agtatgaaat gagcattcag gcggaagact
     4921 tatcttctta tgttcctgcg ttcggttggg agtgctctcc gccgtcggat aagcaaaagg
     4981 cgacccttga aaagctcggt atattccccg acgagataga caatgccggc aaagctcagc
     5041 tgttacttga taggctcggc aaacggcgca atctcggact tactactcca aagcagatac
     5101 gttttcttga aagcagaggt tttcagcacg taggtacatg gcagtttgaa agtgcaagaa
     5161 atctgattga cagaatagcg gctaacaact ggcacgttcc gaacggaata gatcctgcaa
     5221 gctatgaacc gaaggtggtg aataattcag atgtcggaat ttgattttga ccttaacgaa
     5281 gcacttaaat atataagccc gtcagacctt tcctatcagg aatgggtgaa tgtcggtatg
     5341 gcactcaaag aagagggcta ttccgttacc gtatgggata actggtcggc aaatgacaac
     5401 agataccata aaggcgaatg tgaaaagaaa tgggagagct tcaacggctc ttcctcgcct
     5461 gtcacgggcg ctaccatagt tcagatggct aaggacagag gaatgatgtt cggcacggga
     5521 gaagaccgtg aacttgactg ggacgatgaa atatcatacg aacatcacga tgaacacgtt
     5581 gttgtaaaca aaaactggat agagggcaaa gaaataaacg ctccgacaga ctggcagcct
     5641 cacagagaaa taatcagata ccttgaagca ttattcgagc agagcgaaaa tgtcggatat
     5701 gttgtgcaaa gctacgaaaa agacggtaaa ttcatacctg ccaacaaggg ctattatgac
     5761 cgcacggcag gtcagcttat cgaatcattg tcgcagtgtg acggcgatat aggctctgtt
     5821 ctcggtgatt acaacactca ggcgggggcg tggatacgtt tcaatcctct tgacggcaaa
     5881 ggcgtaaaga atgaaaacgt aaccgaatac agatatgcgc ttgtcgaaag cgacaatgta
     5941 gatatagaaa aacagcacgc aatcatctgc gagcttgaac tgcccgtagc tgtgcttgtg
     6001 tacagcggaa agaagtcact gcacgctatt gtaaaggtag atgccgcaaa ttacgatgaa
     6061 taccgtaaac gtgtagattt tctgtatcag atatgtcaga aaaacggact gtcacccgat
     6121 acgcagaatc gtaatccgtc aagattatca cgtcttcccg gtgttcagcg tggtgaaaac
     6181 aggcagtata tagttgatac agacatcggt aaaaacggtt gggatgagtg gcgggaatgg
     6241 atagaaagtg taaatgacga cctgcccgat accgagagca tggctgatgc gtggaacaat
     6301 cttccggaac ttgcaccgcc acttattgat ggtatactca gacagggaca taaaatgctt
     6361 atcgcaggac cgtcaaaggc gggtaagtcg tatgctctta tagaaatgtg ctgtgcaata
     6421 gcggaaggca aagaatggct tggctggaac tgtaccaaag gtagggtgtt gtatgtaaat
     6481 cttgagcttg acagggcttc ttgcctgcac cgtttcaaag atgtatatac aacacttggc
     6541 tgggaacctc aaaatcttag taatattgat atatggaacc taagaggtaa gtctgtgccg
     6601 atggacaagc tcgcaccgaa acttatccgc cgtgcgagca agaagaacta tatcgccatt
     6661 atcatagacc cgatttataa ggttattacc ggtgacgaga acagcgcaga tcagatggcg
     6721 catttctgca atcagtttga taaggtatgc acggaacttg gctgtgcagt tatatactgc
     6781 catcaccatt caaaaggtgc acagggcggt aagcgttcaa tggacagagc ctcgggttca
     6841 ggtgtattcg caagagatcc cgatgcactg cttgacctta cggagctgga gcttaccgac
     6901 agcataataa agcacgaaaa agataagatg acctgtaaga tctgttacga tcagctgaag
     6961 aaatgcggac acgaagacga tgtttcacag gatgatatat gcagtgcaaa gcagatgcgt
     7021 gaagcgctca gaaacgctgt gccggacgca gattataagc atgtgtgtga tttcattacc
     7081 aagtgtgaaa aacgcacaga gagccgtaca gcgtggcgta tagaaggcac gctccgagag
     7141 ttcccgaagt tcccgccggt gaacgtttgg tttgattatc ccgttcatcg tatagacaag
     7201 accgacgtat taaaagacat acagcccgat gacggcagag cagtaggctg gcagaagaat
     7261 ttcagcaaga aaaagaccga aaaggaacgt aaggacgagc gtaaagaatc gctcgaaacg
     7321 gcatttgatg cttgcatgat tgacggcaag gttactttat ccggtatggc cgagtatatg
     7381 ggcgtgaccg aaaagacggt ccgaaaccgt ataaaagagc acggcggttt ctggatcgac
     7441 gataacgagg tagggaaaaa gtcgaagtga aaactttcac tgcgagggaa aatctcggtg
     7501 attttcattt tcactgacga ggaaaatgtc gagaaaattt ctttcactgt cagtgaaaaa
     7561 gtcgagaatt ttcactttcc ctacagagtg aaaaagtcgg tgaattatcg agattttcac
     7621 tgtcagggaa aatgctatat actacgtata taggtttttt ctttccctga cggtcacagg
     7681 gtgaagtagt cgtgcgacag ctacgcacga cgacttcttc ccctgactgt gacaaaagca
     7741 ctgattaaaa atccaaaagg agatgttaat atatgggtaa tagcttgagc ttttttatgc
     7801 cgatgatacc gcctacggta acggcacagg aacacaaagt aacggtttct cacggcaagc
     7861 cgatattcta tgatccgccc gaagtcagat cggcgaaggc taaactgaca gcgtatcttt
     7921 ctcagcataa acccgacaag ccgtataaaa agggcgtaag gctgacgaca aagtggctgt
     7981 ttccgaaaga acagcacaaa gacggagagt atcgtataac gaagcctgat accgacaatc
     8041 ttcagaaaat gctgaaggac tgcatgactg tttgcggttt ctggactgac gatgcgcttg
     8101 tcgcaagcga gatatgcgaa aagttctggg cggcaaatcc cggaatatat atcaaggtcg
     8161 aggtgctgaa atgacgatag acgaagttca gc
//
LOCUS       DAWEMR010000117         8154 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3621_length_8154_cov_16.393135, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000117 DAWEMR010000000
VERSION     DAWEMR010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3621_length_8154_cov_16.393135"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2171..2180
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2568 a         1629 c         1790 g         2157 t
ORIGIN      
        1 gtgtgtgttg tactaccagt ctggcaggaa ggatgaggca aggcagattg cgcagaacct
       61 tatccggaaa aatagtgagg acaatattgc aagacaaatc ctggacggta cctatgtttc
      121 tgaaaacgaa actatcacga tgcttccgga agaagaaaat gaatacaccc tctatgagga
      181 tgcagataat accattccaa aactattaga gcgtatgctg aacgaagtgg aatttgaaaa
      241 cgtgttttac aaaaaaacga ttttggataa ttatgattcc gaaaagcgcc gttacatcgg
      301 cgatgagaac caagctaacg aggacatcat ggaggctctg aatgacattt cccgtccaca
      361 gagtaattat ccactttcag ctcaaagcaa gatttatatg gcgcttgcca aaattggtaa
      421 gcagtttgta gacagcggac agaaaaacaa atacatcaca caaaagctgc tcaatgcgct
      481 gttgggtaaa agcctaatgc tacgagcaga ttcggaggtc aaaaaaagag accagaacag
      541 agacagttca cgatactact actttctggc actgcgatac ttgagcagat gtggggatag
      601 gtataggaaa gactttaacc atgctctcaa tcttctttac tattcctttt ttcagtctga
      661 taacgaactc gcaaacaagg tcgaaagccc agatatggaa atgcttcata tcacaccacc
      721 agactatgag gactcggttt tcaacccaaa agagctgatg gtgtgtacct ttgacctaca
      781 gcttaactat cagaagagta gaataggaga agaaattatc cgtcctatga ttgatgccat
      841 ttacacgcat agccgtctac gcccggcatt ttttgaatgc atggacatga tcacgccaga
      901 aaatcaagaa gtctgccaaa attcagatat attttttgga gcatggagaa ggagtaaaga
      961 tcagtatagc aagcaattac atgatctgaa acatttttta gagaatgccc ctatgaatat
     1021 aaaagaaccc gaaaagctaa aaggcattaa taatagtatt catgaactga atcctgaacg
     1081 cctgctctgt aaaacagata tgatgtatct ggagagaatg agagaaatca tcaaaaaatt
     1141 tgtacagttc aatgaattta atagtgttga cgacagtgtg gacacgctgg acagagtaat
     1201 cgacctgtgt gacacactgg aaaagcaaat ccgcgataca ccaactgact tttcttatga
     1261 aacgttgctt gacatcatca tcgaactgaa aggtagggcg cttgctctga aaaacgagct
     1321 ttacattgaa tccatccccc agctggaggt gtatccggat accaacgcct actacaaagg
     1381 cgaccagatg caggcgaaaa tcacgatcta tattgaaaac aagggcaatg tgcagagagc
     1441 cgatatttcg aacatcagac ttgcaggagt agataatcac gttgaaatca ccggaaatcc
     1501 ggaacatccc gtaacaaccg ttcacggaaa tggtaaggaa gaatactgca ttgacatttc
     1561 ttttaccgag gcggaaaaac aacgagggct gtttgacatc cgattctctt ttgaatacag
     1621 ctattatgtc gaattagggg agatgatcaa gaaaagtttt tcggaagtat tccagatcaa
     1681 tctttccagc atggattcgt tcaagaaaat tgaaaacaaa tacagcagtt ttaccgaaag
     1741 cggtgctgtt acggcgaatg atatgttctt tggacgtgaa aaggagatcc aatacattgt
     1801 agacagtctc aatatgggtg atgggagggt gctttctcac cgcggtatct atatgtatgg
     1861 tcagaaacgt gctggcaaga gcagcatcat ggcacacgta aaagagcgta tcagttcagc
     1921 tttcccggaa cgatatctca ttatcgatct cggaagcatt ggggcaaact cttccaatga
     1981 tggatggctg aaggcgaatc ttatcgacaa acttggcgat gagatcgaag atctatatcc
     2041 ggacatattt cacaagattg aggagtccat ctctttttct gtaacatacc aaaatatcac
     2101 ccaaaacccc aatacacctg aattcgatat gatactaaat aaaattggaa aatatctgcc
     2161 aaacactgta nnnnnnnnnn attatgattt ttgtggacga gtttacctat atctacgaaa
     2221 gcattaagca gaaaaaatgc gatgattcct tcccccattt ttggaaagca ttgctccaga
     2281 attacaacat ctgctctatt gtaatcggac aggatagtat gaaacaattt gtagaagaat
     2341 atcctaatga ctttgcctgc atgaaagaga tgcctgtcag ctatttggat gagcaaagtg
     2401 caaaagaact gattaccgaa ccaatgtgtg acaacggaaa aagccgtttt gagccggatg
     2461 ctgttgatct gatctatcgc ttgactgcag ggagtgctta tctcattatg atcttttgca
     2521 gtgcgcttgt agattatatg aatgaacgcg ggacagcccg tgtgaccaga atcacggtcg
     2581 atacgtttct caacaagtgg ctttttgatt cggcaaagaa caataatcct attacagatt
     2641 ttcattttga tgcacaactg aatgatccct gctactttga aaatgaaaaa cagattacta
     2701 gggacaataa aacactattg acatacattg cacggcattg caatcagaat aaccagattc
     2761 ggatcaaaga tatccattgc acggatgatc tttcggaaaa gagcgctgct tatcaaaagg
     2821 aactgataga acagttgatt aagcgaaagg tgctggttca agacggagag tactgctata
     2881 tcctggtaga tatgttgcgc atatatctaa aaagggggaa tggatcatta tgagaacaga
     2941 taacattttt atcacaggtg gagaggtttc cggcgaacac atgataggat acaaggacaa
     3001 aattgaagca tttacccgga tgattgatca gaagctgtcc gcaacaggag gtatctatct
     3061 ctatgggctg tatcgtatgg gaaaaacctc tgtggcaaag caaagcctga aacgtcttca
     3121 agatgtacat cctaatttat tatgtatcta catgaatctg aatattttca ataccaatag
     3181 cgaaaataga ttcaatagtt ttctggaagc cattataatt gagttagagg agcttttaga
     3241 ggagtcggat gaccccacca tggaacgcgt catgaaaaaa atagctgatt ttaaaatgac
     3301 agataaatcc gagcaaagct accgtattaa attcaacaaa gtgttcacat ggatcaaaaa
     3361 agccagtcgg acagttcttc tgatgataga tgaattttga gttgttcaga gaactggttg
     3421 caagccctaa ttattccgtt tgccttgtaa caatctcacg gcaggagctc agcatgattg
     3481 aacatgaaaa tccaaacaat tcttctttca agggggtcat gtatccatat acaataaaag
     3541 gctttacgga ggctgacatt gatgaatatt gtcgaattat gatggaatac tatgattatg
     3601 atttgagcag gtcggacatt gacttgatac aagcctactg tggttcctct ccctatctgt
     3661 ggtcttgtgt tggctatgag cttgccgaaa agcaacttct gaacagcagc gacttttcag
     3721 ttgaggatat tctgaatatg ccagcgattc tttcaaaaat tggcggatat cacgattcga
     3781 tcctgaaatg cttaacccat gacagggatg aaaacgacat aagtcttgct gacaagcttg
     3841 tcagtgctat catagggccg tcatatctcg caacagatga ggatatcaaa ttgctaattg
     3901 gcatgaatta tctgattgac actggagaaa cctgtcttgc tttttctcaa gcgttcaaga
     3961 aatacctttt gaatatgaca tacaacaatg atgttctgaa caattttgaa acattggaaa
     4021 aaaaacttaa aaatctacta gaagtacata aaagtgaact atttcaaacg ctctttgcaa
     4081 acagttcgca ggatgaaaac ctgaaatggt ttgcggttct gagcgatgta tggaaaacat
     4141 tggagcacaa aacttttgat aaatccaatt atgaaaacca aatcagaaat acgaatcata
     4201 aattcagtgc acacgaaacg gctctaaacg tgatgtcact tgaggatgta acacgcattc
     4261 tgcgtatgca ttggacaatt ttcagttcac actttcataa tcagccgtta agccaatggg
     4321 agacgcatct taaagcatgc ggaagggctc gtaatcctgt tcaccacggt tctatcgaac
     4381 gcatctactc aaaggaggaa cagaagagag ttaattccta ttgcttggag attatcaagg
     4441 aagtatcctg agtagtagtt atcagcatta tattatagat ggaacttgaa tcgaatgatt
     4501 gatcataaaa aaacgcaaaa ctcagaaaga agcggttatc gaaaaactaa aagtattctt
     4561 tgaaaggttt gaaaatataa atgacggctc gaaggcaact cgccctctta gcttattcgt
     4621 cctcatcact gcattcatcc agcccgtgca taagctggat atatacacac tctgctctgt
     4681 catgctcctc gccctcggcg gtcatcatca tttcaaggaa atatgcctta gcctgttcgt
     4741 agtccgtcca gacctcgcgc ttaccattgc atacggtagt gaccttgttt gttctcagca
     4801 ttgccaattc tggtgttttc aacataactg ttacctccaa atcaatattg tagatacagt
     4861 atataccata atagcaccag aatccagcat atcggaataa ttgtaaccat tattgtgaaa
     4921 ggcgcagcaa tttcaaaagc cagtccggta catagcgcac atatcgcatt tcatcgccgt
     4981 cgggaattat cctgacggcg attttcatat tctgctcaat gctttagctg agccaccttt
     5041 gtttgcagaa acatcagcgg acgccagagc ttatcgccaa gctccttgat ctcctcaata
     5101 tcttccttgt aaactttgtt cgtgacattt gctcggtggg caatttggtt gatattgttg
     5161 gcaatgttat ttgcgatgcg ggtgagttct ttcagctcgt tttcgttgac ctgagctatg
     5221 tatcactcaa agatcattgc tcttacaaat gtactgagac tgctcatgcc actgttctca
     5281 aacttcttct tgatagctgc catttcttcg gggctgacac ggactttcag gtacaatgta
     5341 cacttctttt cgtctttcat ttctcttgtt atcctttttt tatcaattcg gagtcgtagg
     5401 agcagcgccc tctgccaagc cttgtactat gtgtgaagtc gcaatttgcg cccacatagt
     5461 accgtgcttg ctactctagt ttcgcccctg tggggcgaat gtcatttccg agcctttcgg
     5521 ctcggtcgtt tttctcaatt tcgagaattg gagctgtgcg actttctctg tttcgtcgct
     5581 ccccattatc aatatagcgc actttcccaa tcacgtcaat aatagatttc agatttttta
     5641 tgttcgttca aaatcgattc gtcaaaataa ccgaatagca taaactgcga aatagcgtac
     5701 tttcagtttt tggaatctca aaattcccct ggacatttgt tgacaaacta tgaacaaaag
     5761 tttgtgcgca atgcggcttg tatttcaaat gtagagcgca ctataattgg atcatggcag
     5821 gtgagcttcg gtcactcggc tcttcctact gagaacaaaa atgaaaggtt gtgatttttc
     5881 tatgggcatc tatacagaga aaagagagca gctcatcgag aagatcaaag ggttggaaaa
     5941 gcgtatcgca agcgatactg cgaaaaagaa aacgctgaag gacgagctga aagaagtttc
     6001 tcgtttggca atggcggagg aatacaacgc aagcggtctg tccgatgacg agctgaaact
     6061 ggtcgaaggc ggaaacgctg agaatgagaa aagcgataca gccaatctcg ccgctgactt
     6121 cggacagcaa atgagcttta cggataaagc gaatccctat gaggattaat attatccgat
     6181 agtaaaaata gctatgcaat gggacggcat ttgccgttcc gcacaactat ttcaaaaaca
     6241 gaaagagggg cagatagaga aagcgatccc ttcgctctaa aagcatagag aaagagaagc
     6301 taaggagtac aagctatgaa taataacact actgccgtag atacagaaaa agcagaaaca
     6361 acttttaacg caacgtccga gtacaagatc ggacatacgc taaatacggt caagaccgta
     6421 ttcaaccctg cttcaatgga aagcctttca gatattctga aacggatcat aatccgtgag
     6481 tgcgaggatt tcctcggtga atccggacaa gaacccgaga agcaggctgt gtaactttgg
     6541 aatatcggcg cattgtattt attttaggtg tgcgctataa tgataacata gcttgctgta
     6601 tctgtcggaa aggaaggtta atatgacaga caagataaca gcgttgtatt gtcgtctttc
     6661 gcaggacgat atgttacagg gtgagagcaa cagcatcacc aatcagaaag ctatcctcaa
     6721 aaagtatacg gaggataacg gtttcagcaa tcccgtttac tacgttgatg atggagtttc
     6781 gggaacaacg tttgagcgag acggcttcaa ggcgatgatg gcagatgttg aagcaggcaa
     6841 agcgggaacg atcattacca aagatttgag cagactcggc agagactact tgaaaaccgg
     6901 tgagtacatt gagatcatat ttcctgacta tgatgtccgc tatatcgcta tcaatgataa
     6961 cgtggatact ctcaagagcg agaatgagct gatggctttc aagaacattt ttaatgactg
     7021 gtatgccagg gatacgtcga agaagattca tgctgtattc aaggcaaagg gtcagtcggg
     7081 caaaccgctt tctctgccta tttacgggca caaggcctct gaggaagata agcacaagtg
     7141 gatcattgat gatgagcctg cagcggttgt ccgcaaaata tacaagctct gcatcagcgg
     7201 ctacggtccg actcagatag ctcgtaagct gactgaggaa ggtatcccaa atccgacggc
     7261 ttacgctata tcgcagggaa gaaacccagt caacaagaat tcaaggcttg accgctggtg
     7321 tactgaaact gtcattgaca ttttagagca ccccgaatac atcggtcata cggtgaactt
     7381 ccgtacatgc cgcaagtcct acaagaacaa gcgtaagatt gaaaatccga aagaagactg
     7441 gctcatcttt gagaataccc acgagcctat cgtcacacag caggagtttg atcttgttca
     7501 ggagatgcgt aagaataagc gcagaaatca gaagcaggaa actgtaaatc cgttttcggg
     7561 catctgtttc tgtgctgact gcggaaagaa gctatacctg tgccgttcaa gaagccttag
     7621 cgaagatcag gagcatctga aatgctctac tcattcagct gataaggata gatgttcagc
     7681 acattatatc aggactgttg tgctgaaaga gttggttctg aatgcactga acaagctcct
     7741 tgataatgtc aaagacaatg aagctgagtt tgtgcagaca gccatggaca actctgcgtc
     7801 aaagcattcc tctgatgttt ttaaggcaaa gaaagctctc aatcaggctg aaaaacgtat
     7861 cgctgaactt gacagacttt ttgcaagact ctatgaggac aatatttcgg gtaagatctc
     7921 tgacgaacgt tttgctgtac tgtccgcagg atatgaggac gagcagaaga aattgaaagc
     7981 gtaagcatca gagcttactg cttttattga aacagcagag cagaaatgtg ctgatgtgaa
     8041 ctcctttata gaggtagtgc agaagtatga gcatatcacg gaacttactc ccgagatcat
     8101 gcatgaactt atcgagaaga tagttgttta cgctcctgaa aagagcagtg ggca
//
LOCUS       DAWEMR010000118         8132 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3633_length_8132_cov_9.172589, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000118 DAWEMR010000000
VERSION     DAWEMR010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3633_length_8132_cov_9.172589"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5601..5610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6652..6661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2217 a         1790 c         1383 g         2722 t
ORIGIN      
        1 aagccgcttt tagtatttaa gcttatttgt tagattaatc ttcagaaaca atttgtataa
       61 actccgccgt atcagcattt acagttccga aaatgccgtt agcaaattca aatgtaaaca
      121 actcggaatt aaaattcttg attgtaagaa tgtcctcgct gtcattgata gaaattgtca
      181 gatcgccgta ttcgttaata ttgaatgtta ccatgtccaa tgtcatttca ttaataatga
      241 tcttgttttc gccgtcgtta tcctcaacaa tatccgatcc gtatccaatt ccgaaaatgt
      301 atgtgtcgtt tccgtttccg ccatagagac agtcgtttcc aacgccgccg tccagaacat
      361 catttccgga accgccattc agcgtatcgt ttcctccgtc gccgtgaata acaatgccgt
      421 tatcgctcca tgctgacatc cagtcatcgt attcagtcgc atggacctgt ttcagaggat
      481 tttcctcgct gtcgtatgca tatcttgttc cgtctgcaaa gtttacattg aagtttctgt
      541 tttcctcaga cgtaaagtaa ttctgaatga tcagcttgtc ctcaattcca tcaaatgtga
      601 tagtaagatc gttccagttc gttctgtaaa cggacatagt atctgctgag agaccgctgc
      661 cgaatgagat cgtattgata ccctcgctgt cgttaattgt atctgtgcca tatcctatgt
      721 tgaagatata tgtatcgttt cctgctccgc cgtagagata gtcactgcct tctccgccgt
      781 caagggtatc attaccgccg ttgccataaa actgatcgtc tccgatacct ccgtacagct
      841 tatctgcgcc gtttcctcca ttaaggctgt cgtttccgtt ctcaccgata agagttcccc
      901 ccctgtcgtc catagttaca atgtaatcac tattttcagt tccgtaaaca gttcttaatg
      961 gactgttgga agcggttgcg tgaaccttag aacctccgtt gaatgtgagg tagaagtttc
     1021 tgtttgcttc ggaagtaaag aatccctcga tcacaagctt gtcatcagaa tcctcaaatg
     1081 taatggtaat atcattccag tttgtacggt aagccttgat ctgatttgac gaatatccgt
     1141 agatctcaat agtatttgtg ccttcaccgt cgccgatagt atctgttcca tagcctttct
     1201 tgaagatata tgtatcgttt cctgctccgt catagagata gtcatttccc tctccgccgt
     1261 caagaacatc gtttcctgca tttcctgtaa gtctgtcgtc gcccttatct ccgtagagat
     1321 agtcgtttcc gtcgctgcct acaagctgat cattgccgtc ctgaccgatc ttggttattc
     1381 cgtcctcata aatagagatc atgtactcgc tgccgtcagt accgtaaata gttctgagag
     1441 gactgttctg agctgttgct tctacgactg ttccgtcggc aaatacaagc gtaaagtttc
     1501 ttgcttcctt gctgatgcag taattcttta ttgtaagggt atcttcaaat ccgtcaaagg
     1561 taataagaat atcattccag ttggaacggt atgcctttac accttctgcg gtgaatccgt
     1621 caccgaacat aatggtattt gcaccctcgc tgtccataat agaatcagcg ccgtatcccg
     1681 gcttgaagat ataggtgtcg tttccgctgc ctccgtcaat aaagtcattg cctgagcctg
     1741 tgtcgataat atcgtcgccg tcaccgccgt tgatgtaatc atcttcgttt ccgcctgaga
     1801 tacagtcatt tcctgattct ccgaacagag agtcatttcc gctgccgccg aagatcacgt
     1861 cattgccgtc atttgctgta attctgtcag ttccggaatc tccgaagata tagtcagatt
     1921 ctgttcctcc gataataaag tcatctccgt taccgcctgc ggcgatattg gctttgtcgc
     1981 tttcaagagg taaataatct gaattctctg tacctgtaac atagttatcg atgcctgtga
     2041 tattatcctg tacgggcagg atttcttcgc ctatctgcag aacgtagttt tcgggagcgt
     2101 ctgcaaaatc ggtaatgata agcttgtcct ctgtatcctt aatactgata atcacatcat
     2161 taccaacggc attgatctcg atatccgaca aagtcagatc gtcggcaagc ttaacagttg
     2221 atgttccttc gctgtcatca atgacatctg taccgtaatc tctgctgaat acatatgtat
     2281 cgtttcctgc tccgccataa agtatatcgt ttcctgctcc tccgtcgaga atgtcatcac
     2341 cgttatcacc gtaaataata tcgtcgcctt cctcaccgaa aatagcgtcg ttatcatctc
     2401 cgccgtaaac aaagtcgttt ccgtttcctg cgtaaacggt gtcattgccc tcgttgccgt
     2461 agataacgtc attttcctta cttccgtaaa cggtatcgtc accgccgaaa gcgtgcatga
     2521 tcgaatcatc tacaactgct ttaagaacat cgtcgccgtt tccgccgtag atatgtctga
     2581 acggactatc cttatctgta acgtgcattt taactccgtc tatttcaagg tcgtaattgc
     2641 ggtattcctc actcttgcgg aaatccttga taacaagagt atcgttcgta ccctttattg
     2701 taatagttgc gtcgtattca tctgtgccgt ttacaaggat atcgcttggt ttaattccct
     2761 taaatctgag agtattcaga ccatcggagt ctattattgt atcgtttccg tagccctttg
     2821 cgaaaatgta tgtatcatcg ccgtagccgc ctttgagagt gtcattgcct gtccctccgt
     2881 cgagagtgtc gttgccttca tcgccaataa gtatatcatt gcccgaatct ccgtacaatg
     2941 tatcgttgcc tgatcctccg ctaattgcgt catttccatt gccgccatga agattatcgt
     3001 taccgctctc accgaaaatg aagtcgtttt tgcttgtacc gttatatgaa tcattagatt
     3061 ctgtgccaag atatgtgttt cctgattttg tagattcaac aatatacgca taattctctg
     3121 aatagtgatt actgtattca gcaaagcatg atgtgccgtt gatcttatca tacgacttga
     3181 ggtaaacacc aagatcgtaa actaagtaat caatattctc tccctcagca gctttttcat
     3241 caaatatgta atttaagaaa tctaagttta gattcttgtt gccattctca tcctcgtatt
     3301 cgtaagtcgc attcagatag ccgccaagac cacagtacat attaagaata ttgtaatact
     3361 gattttctat gtttgtatat atatccttta atattgaagc tgcattagag ttcggatttg
     3421 aacctcctac accctcaaaa tcacgcccca taaactgctc gatcacgtgc agatctcttg
     3481 cgtcgatatt gccgcctcgg ctgttgattg aaatactttc ggaatctgtt aatttataaa
     3541 gaatcttttt caggcagtag cgtttcatag ttatatccgt agattcgctg aatcgatagc
     3601 aaagttcaag taattctccg ctttcatcat cattgatagc ctgaattata tttgggacat
     3661 tacccgctgt aaccacgccg tcctgtgttg tatcagatga atttacaggg aaccagaact
     3721 cgctgatatc caccatcgat acagttccgt ttttgttaat tgtaacgctt gctgattctg
     3781 caatgcgcgt acctgtttct tcatctacaa agctgacttc cgaatgttca aggctaatag
     3841 aaacaatgcc tgtttcattt aaagttttaa gttcatttga ttcggatttt ccgttatggt
     3901 tagcgtctat ccacactctt agatcagcaa aagcaatgtc attattatca attattccat
     3961 cgctgttatc atcaagttct gcaagtgctt caaagccaga ttcggatttt gaaccgtctt
     4021 taagaattac ctgatctccg aaaagctctc cgccgttatc aatatttccg tttccgtttc
     4081 tgtctaacgc taaaaacccg tcctctgtac ctatccatgc tgttttctct gcaaatccgt
     4141 tattgtcaag gtcaaaattt acaccgtgtt caagtgaacg cagttctatg cctgattcgc
     4201 ctaagtctat tatgagagga tcacggggtg gctgggcttt gcctgctctg tcataattac
     4261 cggattcctc ttgaatttct ttcattaatt catctatttt ttttgcgtca tttgcaagca
     4321 atgcttgtct taattcttct gcaaattctt ctcccataaa aacttccaca gctaaaagaa
     4381 aatcatcaac agctttattt acttcttctg ggttttcaac actttcagca cttgctacaa
     4441 ttggatttag tgtaatcata agtgttgtac atgccgcaga aattgatttt gaaacactat
     4501 caagcttgcc ggatgttgcc aatttaatta taccagcagc tgcacatact ccacctaaag
     4561 ctggtatact atactttagt accgttgctg tatctatact tgtactgctg cttttctctt
     4621 gttcccataa ttctaattca cgctgtaaga tttcttgatt tgcgctttca tttgcataag
     4681 aataacttgg tgaatagttt ggtgaataac ttggtgcaaa gtccggcgca aaacttggcg
     4741 caaagtccgg tgcaaaactt ggtacaaaac ttggcgatgg agcgagacca gaaccattac
     4801 caagattttg attaatttga taccaataac ttaaaaatgt cgatccaaat aaccaccaaa
     4861 atggtgacag atcgggaaca tcagacagat tgtcaagttt ataatctgga attcgtataa
     4921 accagtaaag tattaatcca cttggaagat atgcaattaa aatcacatac ttatgagatg
     4981 tgtcataaat accatcaaaa ctaatatcaa gaccctcaag ttgacttata tcaatattac
     5041 caagtttcca actaaatttt ttataaatat ccgctaagct aagatcaccc ttaatatatg
     5101 aaatattttc atcactataa ggtgtattac catatctgtg ttcggttatc ccatcttcca
     5161 gtaaagtatt atttacatac ctgtcgagtt gagttagacc ttcaatcatt ttggaaggtt
     5221 ccaaataaga gcctggtttc acttcccaaa aataagcaat atcgttttca acatcctcaa
     5281 aatataaatc agctcttcca gttcctgttt tgtttccaac tggagcttgt ccagatttaa
     5341 aggtaactgt atgttcatct gtaatattat cttttttata tttatcaata atatgatctt
     5401 gaacatcatg atggaactga ccataaggag gcactatacc atctctttta gccctattcc
     5461 aatctccagt tattgatgtt agtaatactg catcctcttt cgattcttct tcagttctat
     5521 tgtcttctga tatactttct tctgctgaaa caatcaaaga aggaaaattt gatgttgttg
     5581 caacagtaaa aggcggcggt nnnnnnnnnn gatactacca acataaatga aattaatttt
     5641 aaaaattttt gatgcataat aaccagcctt tcattttata tttctaaccc aaacgattta
     5701 agagttcgaa tattgtctgt tctgcgcttc tttaattctg ccgttacctt tgtgtttagt
     5761 tttgcatcat tcaacatttg gtcacgaatg ataatttgct gttgtacctt ctgacgaaat
     5821 ctatttttgt agtcattaat atctgttgct ccggaaaggc cggaaaggcc tttctcaact
     5881 tcttttactt tcattgcaag aatattttcc atatatggcg taatatcacc tttgtaacct
     5941 gtctttatca gtaaaagacc ttcggaatag taataatgag gttttgtgtt aaattcctct
     6001 aaatcgcagc aaatatccat tggggttcca agtttgtaaa aatgctctat gcagttgttg
     6061 agattgattg cttcatcaaa aatcggcagc ataacgtttt gggttacatt aaatgcattt
     6121 ttcataccat aaatgtcgtc tgatttatat aaaaagtgta ctatactttt gctaaaagct
     6181 ccttcaagtg actcagtacg aacaaaccaa tccatacttt ttccccatac atcacataca
     6241 tattggactg cactttccat tacatcatca tcaatctcag gatttaatga acagtaataa
     6301 tgatgattgt ttttcagcca acactccgga ctctttgtaa aatctatcgt tcgcctataa
     6361 agtgaaacaa ctccacctaa aatttcaaaa gacttataac ctgtttttcg tgtccgaagc
     6421 tctcgatatg tcaaaatatg cagtatttca tcatttacca cccgtacaaa gtaagtaagt
     6481 ttagcaactt ttatttttac aaaacccaac ggttcaagag cttctccgaa aacctgttta
     6541 aatgcggcat tcaaagacag ggttctttca ttcttctttt tgaaaaacat cggaataata
     6601 tttgtatctt tgtctgcttt gctttcaaaa tcctcataat cagaaaccat annnnnnnnn
     6661 ntcccagcac ggaagctgct tcgtacaaag cgtcctcaac aaagacctcg ttcttattcc
     6721 agatttcaga aagctgttcc cacgtttttc ccgacactaa aagctgttcc cagcactcgc
     6781 gtctgccctt gggtgaatca tcatccggaa actcggaacg tcccacaatt accgtatcat
     6841 gaatatcagt cccggtataa agttcaatat atgtccagtc gctgtctaca acgtccatac
     6901 tgaaactgct tgttttcatt tctgccgctg tctgctttgc gtcgtcatga gcctgcttag
     6961 ggttgttact gtaatcttca caagtcagcg tcacccattt gccgctttcg gaaaatgcga
     7021 gaatgtatga agatacagct tcgtcttccg agcacggcac aaaaccacgc tttttcatca
     7081 tattgctgaa tgattttata aactgttcct tgctattatt gcttcttatt tgtactgaag
     7141 taaaaaatct gcccataaat tactccttaa attattaaat atttttgtat gaattatgtt
     7201 tatcaaactc ctctaaaaca tatcataaac aatcattttt gtctatagat atctgaaata
     7261 cggtcataaa ttcggcgtaa gcggtcatat ttgctcagaa tttatcgtaa aaaggaaatg
     7321 gcggacaaaa atttgcccgc ctaatgtcaa acaatattac cgttgatgcc tacataaata
     7381 tccactaaat tgctattatt aacacaataa taaaaatccg ttattggctg ataacacttc
     7441 tgagatagat atgtctgaaa cttttttcgt gagtttataa gcgtatcata agttcctgag
     7501 tgccttgctc tgactgcatt gtacagtttg atctgaggct tgtaaacata aaattcatta
     7561 cttttaaact ccttgccgac aggaacaagg aactcaagat cgagtattac ctcatctgtt
     7621 ccgtaagtat tttctttcag cgaaaaaata atatttcccg tctgctccat ttcaagagat
     7681 tccagactgc ttgaaatgtg ctcgatcaac ttaccgagtt tattgtatgt tgatcttgtc
     7741 ctatatgaca gcagcgactc aacagataaa tattgctttt caattatttc cataactatc
     7801 ccatcccatt acttgaaaat gatctgccaa atgtatctcc acatttaata tccctcccca
     7861 ttttttcatt tcgggagtag tataaaacat tttcatcggc tcgtccatag atatctgaaa
     7921 tacggtcata aattcggtgt aagcggtagt ttgaacgtaa ttttattgta aaattttact
     7981 tgctatttaa atagttctat gttataataa taagcaagga ggcgaaatta tgaagattgc
     8041 cgtctgtgat gacgaaatgg tattatttga agaattgtat acacttttga atgaatattc
     8101 tgcattaaaa aaagagccga tattaaccac at
//
LOCUS       DAWEMR010000119         7980 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3724_length_7980_cov_8.420315, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000119 DAWEMR010000000
VERSION     DAWEMR010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3724_length_7980_cov_8.420315"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7138..7147
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2363 a         1605 c         1809 g         2193 t
ORIGIN      
        1 ggacggacgg cagaaagctt gacagattgt gtatgtgcgg gggcgtttat cctaacaaaa
       61 acgattggaa agccgagcgt gagtgccttg atatgaaagg cgacggcata gacggctgtg
      121 tttcagcctt tgtgtataac agtatcaccc ctgaaacaag cgagaaagga gatatttttt
      181 tcaagataat ttcaaatact ataattcgtg tggcgtagct gattgacagg gatatattcc
      241 ctgtgataca atataattat agagataatt gttataggag ggcgaactat ggcagaaaag
      301 gaaaatataa aagataagct tgaaaacctg aaaaaatgct cgacctagtt atggataagg
      361 tcaaaacaag atctgaggac atggaaaaac tgatagctaa atacgaccaa actaatgata
      421 caaaaggaga aaagcatgaa aatatactgc atgagtgata tccacggctg ttattcagca
      481 ctttgcgacg ctttggagct tgtcattgac caccttgacg agcctgacac aaagcttatc
      541 tttctgggag attatatcca cggcggcgag gacaatatag ctgtgcttaa aaagataatg
      601 ggcttgcaga acttatatgg ccataacaag gtggtggcgc ttcttggcaa tcacgaggaa
      661 atggtgatga gcggtttttc ttcggtcaat gaaatgctag gcagcggcga atatgatgac
      721 tctgatgact cgtatagata ttggtttgaa cgtctgccga gataccatgt tgagggcaac
      781 actatctttg tccacgctgg cattgacgag gattcgggca atgcttggga atactgcacc
      841 gaggattata tttttacaga gcagtatcct caccggcttg gcaagataca cggacttgac
      901 aaaaaggtgg tggcagggca tgtccacacc agcagtatag caggcgaccg cagatttaac
      961 gatatctact atgacggagc gagtcactac tacattgacg gagatgtgct tagcacagga
     1021 gatataaatg ttcttatggt agacactgac actgataagt attataaggt aacagagaat
     1081 tacaatattc ctattgagcc ttatgacagt gaagcttggt agactattgt atgccgtctt
     1141 aaaacccaat ataacactaa cgtccccaag ttttcctcgg ggacgttttt tactttgtca
     1201 actgccatga aaagagttaa ttaaaaattc agaacttttg ttcgattttc tgttgtaaaa
     1261 cagaaagttt cgtggtatac tattatacat aaaggaaaaa ggcggtgatg ctatggaaaa
     1321 gacctatgtt gcaatagacc tgaaatcttt ttatgcttcg gttgagtgcg tggaaaggca
     1381 gcttgaccca ttgaatacaa accttgtggt ggctgatgag agccgaacgg agaaaactat
     1441 atgtcttgca gtgtcaccgt cactgaaaag ctttggtatt tcaggcaggg caaggctttt
     1501 tgaggtcatt cagcgtgtgg agcaggtcaa taaggagcgt ttgcagaaag ctcctaaaag
     1561 acagtttgca aagaaaagct atatctattc cgatctgtct gaccctgcct gcgagcttga
     1621 ttatatcgtg gcaactccgc aaatggcgaa atatttggct gtgagtgcaa aaatatacgg
     1681 gatttatctg aaatatgtgt caccagagga tattttcgcc tattctatcg acgaggtctt
     1741 tattgacgca acgggctatc tcgggcttta tagcgttgac ggacgtggct ttgcacaaat
     1801 gctcattttg gacgtgctga aaactacagg tataaccgcc acagcaggcg ttggaacgaa
     1861 tatgtatctg tgcaaggtgg caatggatat tgtggcaaag catatccctg cggataaaaa
     1921 cggcgtccgt atcgcagagc tgaacgaaca gctttacaaa gaaacgctgt gggggcatac
     1981 ccctatcacg gacttttggc gtgtgggcgc aggcacagcg agtcgccttg aaaagcttgg
     2041 tatttacacc atgggggata tctcacgctg gtcgctggat cattatctta tagggaagct
     2101 ttacaaggtt ttcggcaaaa ataccgagct tcttatcgac cacgcatggg gtatcgagcc
     2161 taccgccata cctgacgtga agtcataccg cccctcgaac aacagcattt cctctgggca
     2221 ggtcttgcaa gagccgtgta attatgagcg cacaaggctt attctgtggg aaatggctga
     2281 tatgctgtcc cttgaccttg tggataaggg cgttgtaaca aatcagatcg ttctcactgt
     2341 ggggtatgat aaagaaagct tggcggacgg tcactatacg ggcgaggtgg tttgcgacca
     2401 ctatggcagg aagataccaa agcacgccca tggcactcaa aatctaggca ggcacacttc
     2461 ctcgaccaga aaaataatag acgcaaccat ggctcttttt gacagaatta tcgacaagac
     2521 tttgcttgca agacggctta atctgaccgc ctgtaatgtc ataagagaag aggatatccc
     2581 cgaaattgag agctatgagc agataagctt gtttgatatg gcggaagcgg caaatgctga
     2641 taacaacgca gaagaaaaag aaagggcatt gcaggaagca atgctcttga taaagcacaa
     2701 atatgggaaa aatgccattt tgaaaggcac gaactatacc gagggagcta cggcaagaga
     2761 gcgcaaccgc agtataggcg gtcacaaggc atgaaaggtg ggtcttacaa tgtatgacaa
     2821 ttattatgat ataaagcagt tttcacgtcc tcactatgac gatcatccgc aaatgtctat
     2881 atcggacagg gcggcgcagt tcagcccttt tgcagctttg acaggctatg acgctgctgt
     2941 tgatgaaacc gcaagataca ccgaaatcag ggaggagctg accgaggacg aggtaaacaa
     3001 gctcaatgac gatctcaacc gcctgcttga tatgcttgat gaacgccctg agataagggt
     3061 gacatatttt gttcccgata agagaaaaag tggtggcagt tatcagacca agacaggcat
     3121 agtgagaatt tatgacggct atactaatga gcttgttttt atggacaaga ctaggatagc
     3181 tgtggaggat atgtgtttgg tggagtttgt ggaggagaga ggggaaatac agtagaataa
     3241 accttatttg ctgatttttt tgaattcaga tgtttgccgt aatgtttttg cgtttttttt
     3301 gtggttatgc ggaataagaa acggatagcc gtctgatgag gacggttatc cgtgtttggg
     3361 tatattcagt tactaagcta aatgagaatt tacagtgcag aactgtttat cccgtcaaaa
     3421 gcaaattctt tcgattcatc atcataaata aatatcaatt ctaatcccaa ataatcgtct
     3481 ggatatccaa gatcagttcc aagcagtgct gtttcaatcg aaagaatatc atcttcaaat
     3541 gatactgacc taatatacca ctcacctgta agcaccgatc gtttcgggaa cattccctct
     3601 tcatcattac acagatcatc attgttgata taatcccgta tttcatcttc agaaatctgc
     3661 aataaagttc tttggctatt ttcaaataag gtctttgaaa ccgaggatgt gaaaaaaata
     3721 ctgcggttct aattgatttt catagttaga aaacaggtta tccggatttg gtttggataa
     3781 acgtatcatt tttaaattca taagccacaa ctcctctaaa ttccgattta gcagagaaag
     3841 ctatatcact ttctcattta tctttattat accaaaccgc cccgaaaaag tcaatctgta
     3901 aaccttttgc aaacaaatag cgttgcaata taccgccagt tgtgctataa tcaggatata
     3961 gagcagagcg gaggtgaatc gcaatggata acgaaaacga aaaattagta aactgtgtat
     4021 tatacaagaa aatcaaacgg gtggagagcg cagaaatctc tatcagcaag accatgataa
     4081 aatcggctca acaggataat gacggcaatt gatgatcata ttaaatgaat aggagggttt
     4141 tacttgaccg ttgatacaaa agaggtagtt actactatag cctatatgat aggcgtccga
     4201 atgtcagcac tgaccgtgag ttacggtgag tgttccgagc ttatcgagaa gctccaagcc
     4261 gataaggacg ccactacgat aagatattta tgtaagctgc gaacagtact gatgcagaag
     4321 ttcaaaaaga ccgatgatct tatgcggttt cagctcaaaa atttgcataa catagaatgg
     4381 tacgataagg ataatatcaa acagcttgaa aaatggggat ttaccattat ccaagcgaat
     4441 tgccgttcag agaagtatat gcaggagttt acaagactca tcaatgaaaa tatcgacaag
     4501 tgctctcatt tgttttacga ttggctgaat tgggagtata tccgagatct gttctttgtt
     4561 ccgaaataca acaagaacgg tgtcatgaag caggagttca ccaagtacat ggcgaacatc
     4621 gagcattatc catttcaaat gtatattcac tggcaacctg ccgaagtcgg gagcatcatc
     4681 tattccgacc gtaagttttt gaagatcatc tacggtcagc ataatgacag cttcaccgat
     4741 tatacgaaat atcgggacgc agatgatgaa acacgaaaca atatctataa ttttatcgac
     4801 agcaccgaga aaacagctat agctgtcgat tgtgagaact ctgatccata caagctttat
     4861 agtgttctga aaggcttgaa tcaggaagaa cttgctaaga tcgagaagat cacgctatat
     4921 gacgatccga acactaccgc aggctgggat tggctctcta aattcacgca gataccagtt
     4981 gaacatatag aaatcgaccg agtgacagac agaaagtcac tggtcgatgt gcgaatgacc
     5041 gcaagcgtag tcacggactt ctaccgtgac gggataacat cttttattat tgtgtcaagt
     5101 gattcggact tttggggact tatcgagagc ctgccgaaag caaagtttct cgttatgtac
     5161 gaatacgaga agtgtggaac ggcgatcaaa aacgccctcg cacagcacgg catctattat
     5221 tgtgccattg atgatttctg caccgcaggc acggaggagt tgaaacgtgc cgtactcttt
     5281 gcagagcttg aaaagcattt gccctcactc attggagaga accctctgga gcttactcac
     5341 aagatatatg aagccacaaa agtgaccgct accatgaaag aaatggagaa cttttgcaat
     5401 agatatgtca agacattgcg tctgaaggtg aactccgaag gaaaattcgt gatagagatc
     5461 caaacaatct aagaaaaatt cgcatggctt tctatcaata tcattaagct aataaaatca
     5521 agaaataaaa ttgcgtgaaa atatctaagc cccacggtgt atttaaggtc atcagaagaa
     5581 aaaaagatct tcagataaat ttgtagcaaa tacttcatag ccgtcatctg atattttaaa
     5641 gcgaacaatc cgtacaggca acacataaat aacagtaagc tctgattttt tttgaaggaa
     5701 gaaaataaaa ggtacagtta aaactttttt gcataaaaaa gacttcatac cgctgtgaaa
     5761 cagtatgaag tctgaaaaaa atcttagatc accaacagta gcatacagag tagatcgtta
     5821 ctgtgccgtt tgctgcacct gcgtggatct gatccagctt tctgccgaaa tcggaagtgc
     5881 cgaatgctga cttgcagttg ttgtaagaat actttgcgta gtagtgtccg tttgctgtgc
     5941 ctgtttcgct tggtgaaacc tttgcccact ctgctccgcc tgaccagctc tgaagaacaa
     6001 attctacctg attctgtgtt cctgagtatt caacgtagaa gtagccgttt gactgaatgt
     6061 catatgcgtt gaagcttccg ccattctttg aagtcattgc tgaaacagcc tgaccccatg
     6121 ctgaagcagt tgaagaaccc cagaagatcg attggtaatt gctgtctgaa ctgcttgaag
     6181 aagatgaaga tgaggaagaa cctgatgttg attcaagata ccaaagctgg cactttccgc
     6241 cccagtagct ccactggtta atgtttgctc cgtcagctgt gttctgttcg taaacgtcaa
     6301 ggcatgagta gcagtctgag atctttgtct tgatagcata agcgtcgccg atctttacaa
     6361 gctggaattt ctgacagtca ccgccgtgat actgccactg gttgatgtta gcaccattgt
     6421 ccctgctcca gttgtatacg tcaagacagc ttttgtaatt tgatgcacct gtgtaaatag
     6481 tgtaatagcc gtttccgtcg cttacaagct tgaatttctg attgtttccg cctgtacctt
     6541 tgtactgctg aacgtttgtg ccgttgtcag cctttgcctt gtatacgtca agatacttgc
     6601 cgctgagtgc gctcttgatg taatatgttc cgtcaagtcc gagaactccg ccgttggaat
     6661 ctgttgaagt tcctgaggaa gaaccgcctg aagaagaacc gccggaagag cttcctgaac
     6721 cgctgtaaac cgagcaggtc ttagaagttg ccttaattcc gtttgagcca ttgataagtg
     6781 tatttcccca tgaagaaagg cttgagccgt cccatgagtt tgcaatgtcg aggtagctca
     6841 tgtcagagtt gttgcccttc catgaccagc caagatagcc tacgcccttg cttgtgcagt
     6901 agctcatgat agtagcctca tcaacgtcgc cgtttgtgtg ctgaccgccg aactcgccga
     6961 taacaacagg cacaccgatg ttgagtgcgt tatcaatgtt gtttcttaca gttgatgcgt
     7021 ttccgcctgc gtattcatac atatgaatgg agaatacggt gttcttctgt gggtctgcgt
     7081 tgaaaacaga cttaccgtaa tccttgatgc tgtcaggata ctgtccccag cctgcgcnnn
     7141 nnnnnnncct gcgcagtcta ccataagcat atttgtaatg cctgcgtttc ttacagattg
     7201 gatagcagac ttgtagccgt cagcccatgc gcttccgttc catgtgccgc accactcatt
     7261 tgcgatgttt acgataacat atttctcagt accgctgaga acgctcttca tttccttcca
     7321 gtagtcaacg cacttgttaa gatcataagt gctgtcgctg cctgttgcgt catgtacttc
     7381 aagaatgcat atcagcttgt ttgacttgca ccagctgatg atgttcttta cttcctgtgc
     7441 gcttgttttt gtgtactttg agccgtttgc aagtacgact cttacggtat ttgcgccgtt
     7501 gtttgcagcg cccttgatag aagtttcagt cttctctgtg taccatgcat gagcaatgtt
     7561 cacgccacgc ataacgaact tgtttccgct agcgtcatag atactagtac cgcttacata
     7621 aaatcctgat gccgcctctg cggtcggtgt tgtcataaaa ccgaagcagg ttattacggc
     7681 aaacattaca gcaacaaaag tctgtaatgc ctttgcagaa aaagttttgt tctgtaccat
     7741 aaaaaagctc ctcttataca attatttttt taataccggt attctaaggc agcactgtat
     7801 tatcctcgtg cgttgctgcc ttagctctgt caacgtaaaa cattatatta ttaaaagtaa
     7861 aataagtcaa cttaaatatt tgagtaaaac gtttacacaa ataattttcg tattttagca
     7921 caatttttcg tagattaatt gaacaatatg actaaatttt atcacatgcg cagtataata
//
LOCUS       DAWEMR010000120         7906 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3756_length_7906_cov_9.526812, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000120 DAWEMR010000000
VERSION     DAWEMR010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3756_length_7906_cov_9.526812"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2630 a         1401 c         1562 g         2313 t
ORIGIN      
        1 agggatttac aagtacttta tatttagtta tattgttggt atatgccatt attgccttta
       61 acgacagtat tattaaaaaa ctactatcaa cgatcattcc caatattatt ctgctgctta
      121 tcacttctgt tgaacttaat ttattatcat ctctaaatag aataagcgtc aaggacttga
      181 ttacaaatga taatagcgta agattcatga ctttgattgt aatccagatt tctctttgga
      241 tctccttgaa aattataatc aagctgttca aattctctga cagttataca atatcagatt
      301 ggtctccgat tatcacagta ttaatatctt cctttattct agtatcactt ctccatgtac
      361 tttcactcaa tgcggatgat acgcaaagaa tatacatcaa tttatcatac ctggttatca
      421 ttatactgaa tttcctaatg ttttacgtta tttattcact cttcttcaag aatgctcaga
      481 taaagaatat gaaggtacta tccgtaaaag aacagtatat ggaacagtat gttaataatg
      541 cggagactca atacgagctg atcagaaaaa taaggcacga tataaaggat caacttacca
      601 ccgtttatga attgctcaca agtggcaaaa ccgaagatgc tttggaattt attgaacaat
      661 ccaacggcat tgttaaagct acaatgacat ttgttcaaac caataatcca gttgcaaatg
      721 caataatcaa ctctaaattg tctactgcat caactttagg aataaaggtg tcatgtatta
      781 cggtaaatga ctttgtaggt atcaatgagc ttgatctatg tgacttattg agcaatactt
      841 tggaaaatgc gatcacagcc tgtgaagcta tgccgccaga tttaaacaag tttatctatt
      901 tggaaatcag taaagaaaac aacatttata catttatagt gaaaaattct ctagataagt
      961 ctgttattag cgaaaatcca aaattaaaaa ccacaaagaa agatatcata aaccatggac
     1021 ttggcacttc tattattagg gacatagtaa ataaatacag tggaagatat gattactatg
     1081 aaattgacaa tgctttctgc tgctctataa tcttagaaac ttaactcata tgatagctcg
     1141 gcttatgccg agttattttt ttgtgcctga aaactgcaca atatgccaaa acgcttgacg
     1201 aaagatattt tatattgtaa aattcaaaaa aagcaaggtg aaaattatga attacttggt
     1261 atctcagatt gttaatttta ttacggaaca agaggaagtg attaatgtga aaagtttaaa
     1321 aaaactacta gctgaaatga ttcagaagat tgcaaaacat acagcaattt tggcttgtgg
     1381 agcagcttcg gcatacggtg gatatcagac taaagagcca aagaacatct acaagagata
     1441 agcatcaaaa aacacaattc aaaagattgg catattcgac tattacgatc tacactagta
     1501 tagttttgag ctgaattaat cattttgtgc aggaagaaga ttgattcaaa ggttttcaaa
     1561 gtactgtaat acttgtcata gcaagtatag aaattgtctt ttactttgct taaagatggt
     1621 ttttttagaa tacagacttt aggtcgggag tattgcgttt gtcttatgat gaaagcaata
     1681 aaaaatttca aggaggtttt caatatgaaa cttaagaaga aaattgcggc tatgggtgct
     1741 gctgttatga taatggcttc tatgagtgct ataggtgcta gtgcgtatca agcatcatat
     1801 gaggatagtt atcgaattat acttgcatat acgtcttata ctccaagcga gacagattca
     1861 gtgggaacgg taacgaatct ttcaactaca aaaacaaatt actctgtaca gtcaaccctt
     1921 tattacaaga cttctgcagc aaaaatcggt tcttatactc cttctggatg gaaaaaaggt
     1981 acgctgtcca aaggtcagct tttgaaagca acaacaaccc ataaccgtaa tactagttat
     2041 aagtattctg ccagaactct agtcatgaaa gataatagag tttttaagga tattcagtct
     2101 gattactaat atataaccct gaggagctaa agctcctgcg gcaagcttgc ttcgtcgcag
     2161 ccgatgaaaa gctacgctta catcgggctt tgctccgaat taaatatgca ttaaagaaat
     2221 tttaataaaa atcaattgaa cattttacaa aggcaatgca ttatctgaat aacatcagat
     2281 aatgcattgc cttaatgggt ttgaaggagg aaagaatgta cacgctgaaa aatcttagaa
     2341 atatgatgaa aaatcatacc ggtatgctgg ttctacttgt gatttctatg tttgtttcat
     2401 tcggggtttt gtttttcggt gttggattgt actatcagta cagcaaaaat attgaggacg
     2461 gcgaaattga cagctatgct gtcggttttt ccataaatga cattattaca aaaaaaaatt
     2521 tttgtgagtt tgtgaagaat atgccgaata agcttatggg tgatgtttca tatattacct
     2581 gtttttcgtc cacgcatgta agcggaattg atgaggaaat acctgtcgca ttctatcttc
     2641 agtattctga aggaaaattt gaatattccg ataaagtatt tcagccaatg attgatgacc
     2701 ttgtaattaa ggacggcagt tttttcacgc aggagcaata ttcaaacgga gaaaaaaagg
     2761 ctgttgtaat gggcagcgga aatgtaaatc agccgacttc agcacccgaa tatacaaatt
     2821 cggttacagc attcggtcag agctatgatg ttatcggcac aataaaccct gctaacagca
     2881 gctatttttt ttacagtatc tatgttccgt tcagctcagt ccccgacgat acaattatga
     2941 acgacggggt ttatcttgca ctcaacaata aaatcactaa atctgaatat gatgatttta
     3001 ccgcatatat cagcgaattt ttcaaagata aaatcactct ttatgaaccg gcattcgact
     3061 tggcatcaaa cacttcctac tacataacag ttgtactgat ttcggtaatc attgccattc
     3121 tttcggcatt gaacatttcc attctgtata attacatcat tatcagcaga acaagacaac
     3181 tgaccattat gcgtatctgc ggagggttaa cctcaaatct gagcatatca accgcaaatg
     3241 agattattct tattatgctt cctgtaagca ttatttcagc tttgtgctat gataaattta
     3301 ttcttactgt actttcagct aaatttccgc ttatgaaagc tgcatataat ccatatgttt
     3361 acgcagaaat aattttgatt tactctgcaa tatcgtatct tcttaatctg tttttgcttc
     3421 atcataatct cggcaaaaaa attaaggagg gattgaaatg agaattaagt tatacggcag
     3481 ggttatcggt atcttcctga aaaagcactc gtttatcaat gttttaataa gcctccagct
     3541 gattgctgtt tttgttatcg gaataattat gacatcgata atagacgaaa aggcttcctc
     3601 atatgaggct gtcaaaccgc tgcttaatgg cgagggattg tattgtggtg gatatttttt
     3661 gaaagataat gaaaatggcg gacttttgga aaacacagat gaaatcaaaa ctcagctgaa
     3721 agatgttgat tttatctcgt ctgtaaactt tgcgtccctg caaacaggcg ttgatacaca
     3781 aactaacgag gttaagaccg catatgcact tgtatatgat gattattctg caaatctttt
     3841 tcgcccgaca atagaaaaag gttcgtggat aactgattcc aagcaatccg atgatatacc
     3901 gcaggctgta attacccgaa attatcaagg ctataatgtt aatgatattg ctgaatttga
     3961 aactgaaaat ggaacgttga aggtcagaat aatcggtgta attggtgata atgaaaaata
     4021 ccttggagca aactcggaat atgaatcaaa tgaaccatcc tatcagttaa tgctttctac
     4081 acatttcaac tgtctgaatg accagcttaa aaacaaaggc tttacaaacg aggagattgc
     4141 tttgaaactc gaatctcttg ggtattcagc ggacagcatt gtatataatg aacctgtatt
     4201 gttttttaca gataaggagt ggaataaaac cgatttggat tcagtaatgt cagactccct
     4261 ttttataaag tacaaccgga atatttccga tgatgaaaag ctttacaaca gaaaatatat
     4321 caacgagaat ctttctgtat tatcatttgc gatacagttt tctcagttga acaacaacag
     4381 tcagaccatt gtttatagag agttgtacac attgatgcca ataatgtgtg caatatacct
     4441 gcttgtactg attgcgtcag tgagcgttaa tgcgattaac tgcaaaaata atataagaaa
     4501 cgaagccata ctgtatttgg gtggtgcaaa aagaaaacag ttgcttacaa tcaatgtaat
     4561 ttacaatggg ctgatttgca ttgtgtcttt gataatttca atagcaggat tgttgatttt
     4621 ctacaaggca ggattattca gcaaaacagt aattacattg ggctggaaac cgatcaccat
     4681 ttgcctgatt gtgtgtgctg tatatatctt ggtatcctgt atcatttcct gcattatcct
     4741 cacaccaaag gctttgaaaa aagcaataat attatcggag gaatagtaat ggtaaaactt
     4801 aataacatag taaaaatata taattcaaag aaagccaacg aattcaaggc tctccacggt
     4861 gtttcggcag aaattcggga cggtgagctt attgcgatta tcggaaaatc gggtgcagga
     4921 aaatcaacgc ttctgcatat tttagcctgc attgacaact atcaggaggg cgaatactac
     4981 attgatgatg tgcttgtgaa agacctttcc gaaagtcagt atgcaaaaat cagaaacgag
     5041 aaaatcggga tggttatgca ggattttgca ctggttgagg atttcacggc actggaaaac
     5101 gtgatgatac cgctgaattt ttccaaaaag aaagtcagtg acaagaaaga aaaagccctt
     5161 gcggcactga aatcggtagg cattgaggag cttgccaaaa agctatgcag caaactttcg
     5221 ggtggacaga agcagcgtgt ggcaattgcc agagcgatag tcaatgaacc gtcaatgatt
     5281 ttggcggacg aaccgacggg agcgctggac acaaagactt ccgctgagat tatggagctt
     5341 ttcaagtccc tgaacaaaca gggcaggacg gttgttatag ttacccacga cccgaagatt
     5401 gcggagcaat gcggcagggt tattgagatt agtgacggga atattgttac agcaaaataa
     5461 attgtggagt gaaaacaatg aaaattaccg attatgtaga tctgttctat ccgtcggcgg
     5521 aggctgagga gtatgcaaaa aagcggcaga aaaaatatga aaattatccg caggatttta
     5581 tggtcaatct tgaaacggat aacatagcaa aacttattgc acctatgaat tttgctgtcg
     5641 caacatctaa aatgaaagag tttacagatg acattgaaac ccttgattac aggctggatt
     5701 gtcttgagga ctttatgaat gtccccgaac ttaacggcag atttcgacag ctgataaatg
     5761 aactcgcagg aaacagaatt gacttttcag atgttgattg tgtcaactcg tttatgcaga
     5821 ttaagaccca tatggacagc cttgaagaat ttctcggctg cattgaaaac atcaacaggt
     5881 tctttgcaaa gtataatcac gcaattaagt cggtagcggt aaaaaggctt gcacagtttt
     5941 ttgaaaaact gccaaaaagc gagaacattt tcgaaatagc atacagcatt tcacagctga
     6001 aagatacctt ttcaaaaacc atacggagcg taaaaatcgg tgttaatttt gattcggcta
     6061 tgaatcccga cagtgcagga ctgcttgaaa tcggatatga caaaatttat cccaagggaa
     6121 atattcttga aaagcttgtg ttcaaaagtt ttgcaggcag agagcagttt attggcgagg
     6181 agcatttcaa ctcggcaaca aggcacactc ctattgatat agacactgcc cttttccgtg
     6241 aactgagcaa atacacaaag gagttcgccc tgcggattgc cgcagcactt aaaagctatc
     6301 gttcctcggt tttttcagac ctttcggagc tggaatcaca gcttgacttc tatggcggag
     6361 cggcacagtt tatatcaagt gttagggcaa ggggaatgaa aatgtgccga ccgaaatttc
     6421 tcccctctga gaagcgtgtt acaaggctga aaaacgtctt tgacctcaac ttctacaggc
     6481 agcttgttac gcaaaatccg caggaaattc tgaccgaaaa aattgttgca aacgatattg
     6541 atatgtcaga ttacgcacgg ttttatatgg tgacgggagc aaacaacggc ggaaaaacca
     6601 ctttcgcaag agcggtggga gtatgtcatt taatggcaca aatgggattg tacgttcctg
     6661 cggaaagtgc agagatttcc cctgccgatt acatattcac ccattttcca aaggaagaag
     6721 ctgtcggaat aaattcaagc cgatttacca cggaaatcaa ggagctgaaa aaaatctgcg
     6781 aatatatgac cgacagaagt cttgttattc tcaacgaatc cattcagtca accacgccag
     6841 ttgagtgctt aaacattgca aaaattcatc ttgaaattat agcggcagtg ggggtaaggg
     6901 gattttatgt cactcatcta acagaacttt acaatgaaat tcagcggata aacaacaaaa
     6961 actatcccac aaaggtggga agtctggtat cctgcacaga cagtgagggc aagcgtatgt
     7021 acaagatgaa agccgcaaag ccgcctattg aaagtcttgc atacacggtt tacgaaaaat
     7081 tcggagcaaa gcttgaagac gtacttgcaa agtcgggtga taagaatgga caagcgtgaa
     7141 aaccttttgc agtttcttga ttacggtgga aacgagctgt tttcaagcct tatggaaagt
     7201 gacagcgaca gaccgcaggc gttggacaga atgtgcattt tgcttcgcaa ggctgtgatt
     7261 ttcgggaaac tgaaaggcag agctgcggca taccaatata tgatttcaaa ggcaaaccaa
     7321 atgggattta taaagtcaaa aaaaatacat gcctacaggc tgacagctgc cgaacgggag
     7381 cggattacag aaaaagtctg caagtcaaca ggtgcgaaat attttttcaa aaagtatgga
     7441 aagaacgtga taatttattc tgcactgctt ggggcggctg tgattttggt tgtgctgata
     7501 tataattata tgcactcgga aaaatatctg aaaagaatgt acgaggcggc tgaggaaact
     7561 gtaattatag agtcggcata caaaaccgat tacggcataa agcttaccat acacaacaat
     7621 tcacttgcgg cccatttatc ggtctctata cctagtcctt cttctataat gccaatatca
     7681 ccattagcta caataagcaa ttgatccatt tgattgcttg gtgcaataaa gcgttgccct
     7741 cccgcaccaa gtaaactatc tggatcacta taatacaaat cataatctct ctttgtaact
     7801 aaaaaagaag ctccatattc taaaaaatca ttattcactt cactcataat acactcaccc
     7861 cataatcttt ttttaagaca ttatctatac gaattatgta atccca
//
LOCUS       DAWEMR010000121         7870 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3776_length_7870_cov_12.885221, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000121 DAWEMR010000000
VERSION     DAWEMR010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3776_length_7870_cov_12.885221"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2722 a         1477 c         1621 g         2050 t
ORIGIN      
        1 ccgccgccca tgcagggcaa atggcgagag ctttacgctt atcttaacat aacgagaaag
       61 cttccagctg acataataaa ttattgtgtc agcaataagc tcatatacct cgatgttaaa
      121 aagcgagcta ttttctgcgg ctacgataag tttggctcaa tgaagtttgc agaagcaaag
      181 atcacaaaca catacaacaa atactatcct cagaacattg agggaagtat gaaagagtac
      241 agcttttata tccctgctaa accaaatgcg tatggttatg accctacaaa gctttatgtg
      301 tttgaagcac ccatagacct tttatctcac ggagctttaa tgcagctttc aatgaaaaaa
      361 cagtgtacag cagttggaat gtctgaaatg tatcgttctg attgttggct tggaataaac
      421 agagtatcac tatcaggctg ctctgatata gcacttaaac aaagactatc tgatgatcct
      481 cggatcaaac agatagtctt ttgtcttgat aatgatgaaa gaggacgtca agcaacagaa
      541 aagctaatgt ctgactattc tgcaaattac actgtaaaag tgagcatacc gccttatggc
      601 aaggattgga acgatacgct gaaatttatt gtaacgtcaa aaaacaaaaa aacaaatacg
      661 gaggaataat tatgagaaaa ataattgaaa agatcaaaga aaaaggtgta aagcttagcg
      721 cacgcatttt cggtgcaatg acggcttgtt tttcaacgat cgtggcagct tatgctgacg
      781 ttgaagacac agaagcaggc ggcgtatggt cagacatagc tgcatggata accaaaaaca
      841 aaaatggttt gtccatagtc gcaaaagcac tcattggatt gtgtgttgta tgtatagtta
      901 tatgcctcag cacaagtggt aaacaaggtg tagcttctgt aaaaggttgg cttatcgctt
      961 tggtaattgc cattatcttg ctcgtgtttg cagacagatt tttacagagc atgggagtgg
     1021 gttaaatcac aaggacacac gtttgtgtgt gtccgtaaaa acgcttatcc atagtgtttc
     1081 tacgagcata cacaaacaaa aagcacacga aaggagcata attttgaagt acatgaaaac
     1141 aaaaaaggta tggtatcaac tcatatttat agctgccata gtaacaagtg gctttggaag
     1201 cttggaggta tgggctgcag aacaatcagg atatactggc tgttcttcaa gtattctaaa
     1261 aatggtaact gtccgaaatc agtttcgtga tctgttacgg aatatcgaat ttaacattct
     1321 gagttttctc gcagatgtaa tagacttttt cgataaagcc atcagcgccc tgctcaagct
     1381 caatttcttt gattacttat cagatgaatt cagttttggt aaaatgggaa ctgtcataat
     1441 agcagtaatg agccttgcgc tcgttttagg agcttgtgca ttagtagttt accacaataa
     1501 gatccgtgtg tccgactttc ttatgagcat actcgtttca tctatacttc ttgttgcatt
     1561 tcctaccttt atatcttcct gcaactctct ccgcagcaaa ggtgttgatg cagctcaaaa
     1621 cattaagata aacgatcaat ctgagcaagc aagtgtaaac aatgacggaa cagttgtcat
     1681 aggtacactt ggcaaagatc ttttggcttc aggaatatat gacgttgaaa acagcgttga
     1741 tctaaagaaa aaaatgaatt accttgacac tgtaggaagt tcaggaaata taaaagatat
     1801 cgagataact ggtggtattg cgcctgatga tgataaacca tggactacct attatatcac
     1861 tgatgtaaat gatgtatacc ctgcccagaa aaaatttaat gacctgacaa cagaaaacat
     1921 gatgtccctg ctcggtcttg aagatgaata taatattcta aaaaatgcag acggcgaact
     1981 tatacttagc gctgattatg gcgcaaatat gcaggtttac tgttctgtat acagcacatc
     2041 aggctcaata tacggaagcg ttctgtctga aaaagattat gaaacatttt tatcagcttc
     2101 tattggtgac gcttgtcctt atggtgctac acttggagat ctgcattatc cagacggcag
     2161 caggataaca aatgtcatta actataatac atatcttcgt gaccttatag caaaccaccc
     2221 tgctgtagct gccgcaggca aaacgcagga agttggagca aacgcacaca ctgttgaaga
     2281 agcgcttgat atcataaaaa atgatgtcat aagaaatctt aacatagctg caaacacaag
     2341 gactgctaaa gaagcagagc ctgttaatgc aacatatcaa gcttttccgc tctttgatga
     2401 agaagaatat gacaatctta attctttaga acaagttata agaagaaata ttactagcgg
     2461 atacacatat gacagcttgt actattatca catagacttc tggtcaacat tcgtacttat
     2521 gatagcagta gctatttgtc ttatcttcag cggaataaaa atagcaacta ccctttttga
     2581 gataatgttc tcacagctca taacaccttt tataatagca actgatctta atggctcagg
     2641 acgtgcaaaa aaagctatac aaaatatgct tctctcaaat attgttctta tgatagttgt
     2701 cattctcata aggatatata tttccgttat atggggcgta aaatcttctg attatggaga
     2761 taattttgcc gtaatgctta tagtcataat cgcaggagca aaatttgtta tagacggacc
     2821 tgagatactg actaagctgt tcggcattga tgcaggcgtt aagtcaggtg cagccacaat
     2881 gatggctatc aaccaatcaa tgcaaatgag cagttatgcc acaatgggac ttgctcgtgc
     2941 aggaagcagc gttgtacatg gggcaggaag tgttgcaaag cataccgcaa gcagcgcagc
     3001 aggagcagta aaaggctttg caggcggcgt atatggtggt atacacggcg gtgatactac
     3061 ccctggcaag ataggaaaag ctataggcgg cgcagcagca ggaactgtaa caggcgctgt
     3121 cggtggagct ttcggtcata ctaatgcagg cgcaaaggca gcttatgcag caacaaatcc
     3181 aggctcaata aaagatacat tctcaaatgc aaaagacaac attgccaata acgtaaaaga
     3241 gtctgtagga gagggctttt catcaggcgg cgcaggcagc gcttcaaagg gtgctgacgg
     3301 aaaggacggc ttaaatggca ctaacggcat gaatggagct aaaggcgaca gaggtgcgca
     3361 aggcgcagca ggcaaagacg gcacaaatgg aactgacgca agagccgata acaacagtgg
     3421 aaacagcagc aataacagca ataataacgg cacatctcag cctgcccgtg gcagtggctt
     3481 tgacgtttca aaagctgcaa caaacaataa taacagcaaa tccgacacat caaaaggaaa
     3541 tggatttaag gacaagggag gcaagtaaca tatgggagta gtacctaaaa agacaaaagc
     3601 ggaacagcgt atcttccgcc atatcaacct tacaaaaata gtcggattga tagtaacact
     3661 aatggtgtca tcacagttag ccaatatcat ggtaagtccc aaactaggca ttccatttat
     3721 catattctgt agtgtgatat ttctcataat gagcggaaaa agcccctctg atccatgcaa
     3781 aagctttgct gaagcatggc ttgagtggct cagtttccta atgacaaata aaactctgca
     3841 taagcaccaa ggaggagaga tcaatgatga ggagatcatc gacgccgaaa agcacgaata
     3901 acaaggtcaa tggtaaaggt ttttcaaaaa gaaaagcaca taagaaagct gccgaagacc
     3961 aatcgagtat aatatcacgt ctttcatttg aaagcattga aaacggcatt gtgacgctta
     4021 aggtcaaagg aaaatcactt aaaatgggtg ttcttgccgt atcaggcatg gatatattcg
     4081 atcttaacga ttatgaccgc aatacagttt tcaataattt tgccaaggca actttatcta
     4141 taggcactga tcacaaatac gtttttactt caaaaactcc gtatcttgct aatcagaaag
     4201 cctatataaa gtataaaatg caaaaaagca tcaataggta ttcagaatat ctgctcaacg
     4261 aacagcatga tatatttgaa gattttgagg taagccatta tgatcgaatg gcttaccttt
     4321 ttatttactc tgatgacgag aagaaaatat atactggcgc tcagaggttt ataaaccaca
     4381 tgagagatgt tgatgtatca tggtgttcaa ccgaagaaat aatcgttacg ctcaataagc
     4441 ttatctgtct gaatacagaa aatcagcagt tgtccgaaaa tggggatctt aatgaaacta
     4501 tcctgcctga aacaataata tttaagccaa attattacaa gataaatgat gtgtttgctc
     4561 agacagtggt agtgtacgat tatgcagctg agatagatga ccttagactt gctcagatag
     4621 ttacacaatc gaatgatacg atagtatggg acgttaatgt ggctgataaa gaaactgtat
     4681 tagatgaact tagttcttcc ctgcgagagt tagagtcacg aggaggtata attcaggacg
     4741 ccacagaaaa gctcgataca agcactgaat atcagaagct tgaaatgata taccagaaca
     4801 taaaaaacgg caatgaacag atatactatg taacactcag atttattgta agtgataccg
     4861 atctaagctc actcaataaa cggacacagg aactgatcca cgagcttgaa ctcagcggtt
     4921 tggcggcgta tgttccaaca aatataatgc agcatgagtt tttaaccact attgataaaa
     4981 gcaacacgat aaaaacacct ttcccagtgt ttgatacctt gtcacgtcag tttccgtttt
     5041 actatcaatc acatatagac gatacagggc ttttctttgg cttcacggaa acaggcggtc
     5101 ttaatattct caataccttt aaacggacta aaagtcgtaa ttcctttgac cttttggcaa
     5161 taggacttaa aggcggcggt aaatcagtaa cgctgaaatc aatgcttgaa gatcagttgc
     5221 ttataggaaa taaagtaatg gtactcgata tcgaaagcga gtatcaacct atggcaaagg
     5281 tctatgacgg tcagactata aagataaaca gcaattcaag aataaatcct ctgcaaatat
     5341 gtaaagttgt agactcacga gttgatgatg agatatcacc agaggacgaa gcaagagaaa
     5401 attttgcttc tgaaatgtca agaatacgca catttatgtc aatgtatatg ccagaaatag
     5461 atatgtacac tatggaaatc ttcatggatc ttgtaactga atgtctgtcg gacaagggta
     5521 ttttccctga aacagatgtt acatatcttg atccgaaaca gtttccgtct tttacagatg
     5581 taaacaacaa gcttcacgaa aagctcagca acaggacagg tatgccagaa agaaaatatg
     5641 aagcatacag caacattcag gtgtatataa aacagctttg cggtaaaggt gcatatgcag
     5701 gaatgtttga cggagcgact aatgttgatg taaaaaacaa tgacctgata atctttgatg
     5761 ttaagagcat aagtgaaatg gctgagaatg tatacaacgc tcagcttttt aatattctca
     5821 ctattatgtg gtcaacgata tgcaaaaaca taggctgcaa tcaaaacctt atcaatccat
     5881 gggataaaag aaatgttgtg tgtcttattg acgaagctca cagatttatc tcagtgaaat
     5941 atccacaggt aacagagttt attgaaaaac ttgttcgccg cacaagaaaa tattttgcag
     6001 gattatggtt tgcaactcaa tcaatattag actttattcc tgacggaaat atctcagcag
     6061 caggctctat aaaaatcatt ttctctcttg ttcaataccg attggtgctt aaacaatcgc
     6121 ctgaaagcat tgagatattg caccaagcct ttccgcaatt ctcatatgcc gagcttaaag
     6181 aaagcacagc atttgagcct ggtcaaatgc ttttgtcatt aggagcaggc agggacaagc
     6241 tgcattgcag aagaattgtt ggcgcaagac agctcctcta tatgggcaat gcagaggaca
     6301 gaatagagat aatccacaac tgtttttctc actattacaa cgaatattct aagcaggaat
     6361 atggacttat gttaagaaaa atggatgctg attatttcag gaaatgcttc cttgcagaaa
     6421 catattccta ccttagaatg gagcaaaaca taagtcatta tattgacgca gttatagttc
     6481 aaatggtgga taatcttatc aaagaacttc ttcaagctgc aggaacggag gcggcacgat
     6541 gaaagtagta agtcctaaaa gcagcagtaa aatatatata atatttactg ttgtgacagt
     6601 gttgttagca gcattttttc ttacgtcgaa cagatggatg caatcaatta catacaaaag
     6661 caaaagcgat aattataatc agacgctaac tcttaattgc tacaatataa agataacaga
     6721 cgcttcttac cacgataata tttgcgaatt tgttttaaaa tgccgccttt ctggcagtga
     6781 tactggggca acatatcccg aaattaaggc gataagattt gatgatgatc taacagagta
     6841 taagttcaca ctcagcgaaa aatatgatga ttattcaaga aaagtaacag tgaaagaccc
     6901 tccgtccgac ttcaagataa tgcgtgttgt tgtcagatct gctctgccag atacaaaata
     6961 tgcggacgca tatgatgaat ttggagagaa gatacctgca catactgaga aagggaaagg
     7021 gtacattaga acgataacta ttgaccgaaa agatatgcag aaaagtgcaa taaaaatcgt
     7081 cccaggcaat actgaaagcc gtacacaaac gagtacagca agcagtattt caagcacaaa
     7141 aagtccaata tccacaacag cacaagctgt cgctgtgact acaaaggcta catcacaaac
     7201 aacacataac gtcagcgaaa aggcggcaga agagtcacaa acaagcacaa ctacttcaac
     7261 caaacatcaa gctgcaacag cagaaaacaa cacaagaccg caggaaacaa gacgttatga
     7321 tgagcctgca tatgccccag aacagaccac tggcgcaaca cataatgagc ctgctcaaac
     7381 acttgcgccg tctacacaac cgccagagca ggtaacaact acaacaacgc agcctagagt
     7441 ctattctata aagcttgcta cagattttga gtataatgac gttcagctta aagtgggagg
     7501 acgcactcag cttcgtgcag agatcgagcc tgacaatgcc attaataaga acgtaaaatg
     7561 ggagagtaac cgtcctgata ttgcaacagt tgacagcaac ggcaatgtaa cagccgtatc
     7621 aaaaggcaaa gctatcataa ccgcaacaac agaagataag ggcttaaagg caagctgtat
     7681 gataacagtt agccaataat gaaaagaggt aatcagaatg acttttaata attcttcaaa
     7741 caaagtaaga gtaataggtt ttatcgtttc tgatataaaa aacctaagtg cctatgctat
     7801 cgaatttctt atcgcagtgc ctagaaaggc taaaaaaggc gagcagagct ttaacgatgt
     7861 tttcactgta
//
LOCUS       DAWEMR010000122         7812 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3815_length_7812_cov_12.505221, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000122 DAWEMR010000000
VERSION     DAWEMR010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3815_length_7812_cov_12.505221"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2344 a         1526 c         1840 g         2102 t
ORIGIN      
        1 cgtggacgct atggtaaagg ctataaatga cgctgtggca aaccttgtaa agaaagcacc
       61 tgcttcttca cagcctgaca gcgtaagcag ttccgcttct gacagctcat cttctgacag
      121 caagtcagac agcagctcaa aggcagcttc aaacacaaac ccatcgaccg gcgttgctgg
      181 aggagctttc gctcttgcac ttctgagcgg tgcggctgtg gtcatggcta agaagaataa
      241 gtaatatatc cattaaatgg atatataaag caattaagtg ggactttcat acattatttg
      301 tttaaatgaa taccgcttgc gcagattttt gacggcttgt gtcaaacttg tcgacaagtg
      361 tgtgggttgt gttaaaataa tcgtgaaaaa tttatgttct gagtaaaaaa atatcaacac
      421 cgaaaaatat tatggaggaa ttagaatgaa aaagaaaatt ttaagccttt tggtgacgat
      481 gggtcttgcg gcaagcttag tgcctgtttc tgtttttgct gacggtacag aggtctggga
      541 cggctctgta gcggagtctt ttgcaggagg aaaaggcaca aaagatgacc cttatcaaat
      601 agctactggc tcacagcttg cgtattttgc gaaaagagtg aacgcagagg aatatggtga
      661 aaaatatgca gacacatact ttgagcttac agaggatatc gacctcggtg gaaaagagtg
      721 gactccagta ggagaaactg tggcggatct tattatgggt ggtcatgaat attttgtttt
      781 ctctggcaac tttgacggaa atggttatac tatcaaaaat cttaccattg gaacagaaac
      841 atcgccatac agtggcgatg tatgcggctt gttcggtgcg accagcggca ctatcgagga
      901 tgtagttctt gaaaatgttt ctataaacta tgttggcgga aatcacagca gtggatatgg
      961 cttccgcatg ggaggtgcgc ttgtaggtta ttctatgggc gatatagtaa attgtactgt
     1021 tatcggtctt gacatgaaag caggctctga cggcagctat gttgctctta acagcatagg
     1081 cggattagta ggcattcagg acggaggtac aacagtgagc cacagccgtg tttccggaaa
     1141 gatagaggaa tcaacaaaaa agggcaatgt tggtggcttt gttggtacgc tcgcaaaagg
     1201 ctcatctgct aaatattgcg gtgcagacgt ttctgttgag gtaacaggaa atggtcgtgg
     1261 tatcgcagta ggtggcttcg ttggaatagg taatggagtg acaattgatg aaactttgat
     1321 agaaaattgc tatgccacag gcaatataac aggtgcagaa tatgcaggcg gctttgttgg
     1381 aaacatctca ggtcttaaca tcagcaactg ctatgcaaag ggtgatgtaa gcaatagttt
     1441 tgttggtgcg tcattcatgg gtactgacgc tgcaagcaat aactattatg gcactgtaaa
     1501 gaattgctat gcaacaggtc ttgtatctga tatttcttct tccgcttatg cttttgcaat
     1561 gcaagatacg atgaagagaa gtacgattca aaattgctac tacaacatac aaaatacagc
     1621 aaaaaatacg gaatcagcag cttctcttac tattgacgat atgaaaacag acagcttttt
     1681 aaatgtgctc aacggctcat caaacgtatg gactaagcgt gataatgata ctcctgcctg
     1741 cggtgctgag cctgctgact acagcaatgt ttatgaagtg ctgggtaata ttcctgccga
     1801 cctttctatc tacacagatg aaagcgttgc aaacttgtac agtgcagttg aggctttaag
     1861 agggtataga gatcttgcac agcagtcaga tgttgacaaa atgacaaatg atataaacga
     1921 tgccgttgaa gcacttgttt acaagtcagc agactacaca gagcttgaca aggcagagga
     1981 agccgcaaag gctctcaaca aggacgatta tgaggacttc tcagaagtcg aaaaggctct
     2041 tgccgctatc gacagaacaa agaacatcac agagcaggct gacgtggacg ctatggtaaa
     2101 ggctataaat gacgctgtgg caaaccttgt aaagaaaaca cctgcttctt cacagcctga
     2161 cagcgtaagc agttctgatg cttcgtcaga tacgagcaat tccgcttctg acagctcatc
     2221 ttctgacagc aagtcggaca gcagctcaaa ggcagcttca aacgcttcaa acacaaaccc
     2281 atcgaccggt gttgcaggcg gagctttcgc tcttgcactt ctgagcggtg cggctgtggt
     2341 catggctaag aagaaaaagt aatatatcca ttaaatggat acacaaagca attaagtggg
     2401 actttcatac attatttgtt taaatgaata ccgcttgtgc tgatttttga ccgtttgtgt
     2461 caaaatagtt gaaaaattat taccgctctg ctatcataat tataagcagg gcggttttat
     2521 atcgcaatgc aaatatgata tgaagtctta tatttcggga ggataaaata tgaaaaagac
     2581 aagaattttt agcacaatgc tcgcaacggt catatgtatg gcatctttgc ctgcaataaa
     2641 tgtttttgcg gcaaatcagc agagaactac aactcttgac cttacagtgg caggatttca
     2701 gaacgatcag aaaaatgaag acgagggctg gagctgggac gcagcaactt ctactcttac
     2761 acttgacaat gttgattttt cgacggcgaa aaagtcttgt gtcattgttg acggagaaaa
     2821 agttactaat atagttttct caggagataa caagatgacc tcaggcacta cagttatttc
     2881 gagaaaagga agcgcaaaag ataccggtgt agtactaagt ggaaagacta aggacagcgt
     2941 gcttaatctt gaagaaacag gcaaccttcc agtaatggat cagcctaata ttacttttga
     3001 gtcaggaact gtcaacgcaa agggcggtgc tgtcataaca ctttattcca taaaggtaat
     3061 ggacgcaact cttaatattg acacaagtga agttgccaat ggtggctgga atgacggttt
     3121 gtatgcaaac ggctctgttg agatatacgg cggagatgtg aatatcaatg caggaagagc
     3181 aggaatatta gtagttggta taggtgcccc tgagcctaag acaggcttga ttataaagga
     3241 cggaaaggta gatatcaatg ccaagcttgc ggatatttat cttggtacag acaatataaa
     3301 gaacggtctt atctcgggag gagatatcac actcggtggt gacataggaa ttttccttaa
     3361 cgattgcgaa aagtgcgaga taaagggcgg tacattccat actgatgaat gtgaaaaacc
     3421 attcgcagtt catagagaca gttcggctgt atttgagtat gcaaaggcag attatacaga
     3481 gcttgacaaa gcagaagaag ctgcaaaggc tcttaataag gacaactatg tggactttac
     3541 agccgtagaa aaggcacttg aagctatcga cagaactaaa aaccttacac agcagtcaga
     3601 tgttgacaaa atggcgaaag atataaacga cgctgttgag gcacttgttt acaagtcagc
     3661 agactacaca gagcttgaca aggcagagaa agccgcaaag gctctcaaca aagacgatta
     3721 tgaggacttc tcagaagttg aaaaggctct tgccgctatt gacagaacaa agaacatcac
     3781 agagcaggct gatgtggacg ctatggtaaa ggctataaat gacgctgtgg caagccttgt
     3841 aaagaaagca cctgcatctt cacagcctga cagcgtaagc agttctgatg cttcgtcaga
     3901 tacgagcagt tccgcttctg acagttctgc ttccgacagc tcatcttctg acagcaaggc
     3961 aactgacagc aaagcagaca gcagctcaaa ggcagcttca agcgcttcaa acacaaaccc
     4021 atcaaccggc gttgcaggcg gagcattcgc tcttgcactt ctgagcggtg cggctgtggt
     4081 catggctaag aagaaaaagt aaataagatc tcccctattg ttgatatttc ggagaagggc
     4141 agttccttgt ggactgcctt ttttctgttt gcaggaaagc ggaatttgcc tgcataattt
     4201 agggcgtttt tttgcaaaga agcataaaaa tgcaattatt ttatattgat actgcaaaat
     4261 cgtccgcgca agcttagact aactattgat aatagtcaat atcttttatt atctatttta
     4321 taccatgaaa ttacatttat tcataaaaaa tcgttataga tttaagcgaa atgtaaaaaa
     4381 tcagctcaca aaatctgcgt ttacccttgc aattcagaaa aatatgtggt ataattatat
     4441 tgctgtaata ggagcacagc atgaaatgct ggcagtgtgt tttcaattgt cagatgaatt
     4501 tctgtatagc ttatgcttta taataaggaa agaaaatgaa tgtgccggcg tgtgctgttt
     4561 cattatagcg cctattaacg atattttttt tgaaaaggag ttattgcaac atgtcaaaga
     4621 tcgatttgag caagtacggt atcaccggaa ctacagaagt tgtttacaac ccttcttacg
     4681 agcagctgtt cgaggaagaa acaaagccaa cactcgaagg ctatgaaaag ggtcaggaga
     4741 gtgagcttgg cgcagtaaac gttatgacag gcgtttatac aggccgttca cctaaagata
     4801 agttcatcgt tatggacgaa aattctaagg acactgtttg gtggacttct gacgagtaca
     4861 agaacgacaa ccacccagct tcacaggagg cttggaagtc tgttaaggaa ctcgctatca
     4921 aggaactttc aaataagaga cttttcgtag ttgacgcatt ctgcggcgct aacaaggata
     4981 caagaatggc tatccgtttc atcgttgagg ttgcttggca ggctcacttt gtaacaaata
     5041 tgttcatcaa gccaactgct gaggagcttg agaactttga gcctgacttc gttgtataca
     5101 acgcttcaaa ggctaaggta gagaactata aggagcttgg tcttaactct gagacagccg
     5161 ttatgttcaa catcacaagc cgtgagcagg taatcatcaa cacttggtac ggtggagaga
     5221 tgaagaaggg tatgttctca atgatgaact acttccttcc actcaagggc atggcttcaa
     5281 tgcactgctc agctaacaca gacaagaacg gtgagaacac agctatcttc tttggtctgt
     5341 ccggtacagg taagacaaca ctttctacag accctaagag acttcttatc ggtgacgacg
     5401 agcacggctg ggacgacaat ggagtattca acttcgaggg cggctgctat gctaaggtca
     5461 tcaacctcga taaggattct gagcctgata tctacaacgc tatcagaaga gatgctcttc
     5521 ttgagaacgt aactcttgac gagaacggca agatcgactt tgctgataag agcgttacag
     5581 agaacactcg tgtatcttat cctatcgacc atatccagaa cattgtaaga cctatttctt
     5641 ctgcacctgc tgcaaagaac gttatcttcc tttcagcaga cgcatttggc gtactgcctc
     5701 cagtatctat cctcacacca gagcagacac agtattactt cctctctggc tttactgcaa
     5761 agcttgcagg tacagagcgt ggaatcactg agcctacacc aacattctca gcttgcttcg
     5821 gtcaggcttt ccttgagctt cacccaacaa agtatgctga agagcttgtt aagaagatgc
     5881 agaagagcgg tgctaaggca tacctggtaa acacaggttg gaacggcaca ggcaagagaa
     5941 tttctatcaa ggatactcgt ggaattatcg atgctattct taacggcgat attctcaacg
     6001 cacctacaaa gaagatacca cactttgact ttgaagttcc aacagagctt ccaggcgttg
     6061 atcctgcaat tctcgatcct cgtgacacat atgctgacgc ttccgagtgg gagacaaagg
     6121 ctaaggatct tgcaagcaga tttgttaaga actttgctaa gtatgagggc aacccagcag
     6181 gtaaggctct tgtatcagca ggtccaaagg aataatcaat aactctgata ctattatata
     6241 gtataaaagc tgctgtgcct tttggtacgg cagctttttt gcgctcttga aaaagtgtga
     6301 tgaaggtggt ataattggta tggaggcgaa tttagtttgg aaaagaagaa ttatgccgca
     6361 agggcggcta tatatgccat ggggcttgtg atacttgccg cagggataac gatgaacatc
     6421 aaaacagggc taggggtatc cactattata tcagtgccat ttgccatatc gagtatatgg
     6481 gatgtgaatt tttctcttgt gacttttgct acatatgcac tgttcgttgt gatagaaatg
     6541 ataataaaag gcagcgacag aactgttata gactggctgc aaataccatt cagcattgtg
     6601 ttcagcctgc tgctgaatat gttttcaaag ctgcttgatt ttaatttcgg acatttgtgg
     6661 caaaacacgc tgtatcttgc agcagcgata atctgcacgg gtgctggaat gtcaatgatg
     6721 atagccatga agttcgtgcc gaacccagcc gacggtcttg ctcaggctat aggaaaagcc
     6781 tttggaaaga acatgggctt tggcaagaat atacttgacg catcgtgtgt tgccattgcc
     6841 tgcgtgatag gtctgctgtt tgacaagcgg ataataggta tcggtatcgg aacggtggcg
     6901 gcaatgctcc ttgtgggaag gtgtgtggca gtattcgacc atttcttcaa agacagaatg
     6961 aaaagtgccg cagggcttgc agaataaaaa acgccgcaga ggacaaaaac ttctctgcgg
     7021 tattttagta tttatgcaat aaaaaaatcc gctctacata acgtaaagcg gatacaagca
     7081 aaagcttaaa cttttgctgg cgcggattga gagatttgaa ctctcgcgcc cgtttccaga
     7141 cctactccct tagcagggga gccccttcac cacttgggta aatccgcaaa cctgtatata
     7201 taaaaaatgt ggcggacagg gtgggattcg aacccacggt acgttgccgt atcactggtt
     7261 ttcaagacca gctccttaaa ccactcggac acctgtccac attaattatt tgtttttgtg
     7321 tcggtctctc tcagcgactt aattattata gcacagttca tctgtcttgt caaccccttt
     7381 ttcaaaattt ttttgcgaaa ttttttaaag cataaatatt ccttcgtatg ctaacgatac
     7441 ttgacgaaat cttataaata tggtataata aatcacagat ttgtatgttt tatcacttta
     7501 tatgataatt tatacagaaa attttttatg gaggcgactt tattatgaag atgactgtaa
     7561 gtcaggctat ggttgaatgt ttgaaagctg agaacgtaaa agtcgtcttt ggctatcccg
     7621 gagctgctat ctgtcctttc tatgacgctt tgctggatgc tgaaaacgac atcaggcagg
     7681 tgcttgtgag acaggaggct aacggtggtc atgcggctaa cggctatgca cgaatttcag
     7741 gcaagcctgc ggtctgtatc gctacatcgg gtccgggtgc aactaatctt attactgcta
     7801 gtgctaccgc tt
//
LOCUS       DAWEMR010000123         7706 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3902_length_7706_cov_10.934257, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000123 DAWEMR010000000
VERSION     DAWEMR010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3902_length_7706_cov_10.934257"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2306 a         1498 c         1858 g         2044 t
ORIGIN      
        1 atccgtatta aaatggtatg ataaatatgt atttttataa aatatattat ttgcagaagg
       61 attgatatat aatgaaaaca ccatttataa ccaaggaaaa ggccgaggag ataatcaagc
      121 agtatcctac accttttcat atctacgacg aaaagggtat cagacagaga gccagagatc
      181 tttacaaggc atttagctgg aataaaggct tcaaagagta tttcgctgta aaggctaccc
      241 ctaacccatt cattttgaaa atactcaagg aagagggctg cggcgttgac tgttcttctc
      301 ttactgagct tatgatgagc gactgctgcg gattcagcgg aagcgacatc atgttttctt
      361 caaacgtaac tccaaaggcg gattttctca aggcaagaga gcttggcgct tatatcaacc
      421 ttgacgattt cacacacata gattatcttg acgacctttg cggtattcct gaaactatct
      481 gctgccgttt caatccgggc ggacatttcg agatagaaac tcagataatg gacaaccctg
      541 gtgaggctaa gtacggcttc acaagagagc agctttttga gggcttcaag atacttcagc
      601 agaaaggcgc aaagcatttc ggacttcacg ctttccttgc ttcaaacact gtttcaaacg
      661 cttattatcc aaagcttgca agaatacttt ttgagcttgc tgttgagctt catgaaaaga
      721 cgggtgcaga catcaagttc gtaaaccttt caggcggtgt tggcatagca tatgagcctg
      781 acaagccaca gaacgatatc ttagctatcg gcgagggcgt tcgcaaggca tttgaggata
      841 taatggttcc tgctggtctt ggggacgttg ctatcttcac agagcttggc agatatatgc
      901 ttgctccgtt cggtcatctc gttgctacct gcacacatca caagcatact tataaggagt
      961 atgttggtct tgatgcttgt gcctgcaacc ttatgcgtcc tgctatgtac ggttcatatc
     1021 atcacattac agtgcttggc aaggagaacg ctccgtgcga ccacaagtat gacgtaactg
     1081 gcggtctttg cgagaacaac gataagtttg cgatcgacag aatgttgcct gagatagaca
     1141 caggcgatat aattgttatc cacgatacag gcgcacacgg tttctcaatg ggttataact
     1201 acaacggaaa gctccgttca gcagagatac ttcttcatga ggacggctca ttcgatatga
     1261 taagacgtgc cgaaactcct gctgactatt tcgcaacatt tgatttcact gatattttcg
     1321 ataaatatac aaagaataat aagtaatggt gcttagatcc acagggaatt tctctgtggg
     1381 tcttgctgtt ttcagggcgt ttggtgagca aatattaaaa aaatcggaat ttatgtgatt
     1441 tttgcttgca aaatagtatg agatgtagta taatattaaa ttgaattggt gtatttttgc
     1501 acctaaggcg ttgccttaat attaagaaag gtcagggatt atatatggat ttgaacatgg
     1561 tcaagtatct ggcagagctt agcaagctgg aatattccga taaagagctt gcagccacag
     1621 ccgagcagat gactgatatt attgatctta tggataccgt aaaggaaatc gacattacct
     1681 atgacgatct caaggacaat catgatgttt acctcaacga tctgagaaaa gacgaggtca
     1741 tcgaatctat gcctacttca aaggtccttt caaatgcagt gaatacaaac aactgctttg
     1801 tagtgcctaa ggtcgtagag taaatttaac aggaaggtag tgtgaaattt ggacgtttct
     1861 tcaatgaaaa tccgtgatat gcgtaaggct cttgacagta aggaaatttc cgctaaagag
     1921 cttgcacaga catattttga gagaataaag aaatatgacg ataaggtaaa gagctttatc
     1981 actgttactg aggaaaaggc tctggagcag gctaaaaagg ctcagggcgt tatcgataag
     2041 ggcgaagcaa aggctctcac aggtatacct gtggcagtaa aggacaacat ttgtacagag
     2101 ggcgtaaaga ccacctgtgc atcaaaaatg cttgaaaact tcgttccgcc ttataatgcg
     2161 accgttatgg aaaagctcga cgctgagaat atcgtaatgc tcggcaagac ttccatggac
     2221 gagttcgcaa tgggaggctc tacacagacc tccgcttttg caaagacaaa gaatccttat
     2281 gatcttgacc gtgtacctgg aggctcatca ggcggatctg ctgcggctgt cgctgcggga
     2341 cttgcacctg ttgctcttgg tactgataca ggcggatcta ttagacagcc tgctgctttc
     2401 tgcggcgtaa caggtatcaa gccaacatac agcagagttt caagatttgg tcttgtggct
     2461 ttcgcttcat ctcttgacca gataggctct atcggtcttg acgctcatga ctgtgctata
     2521 ctccttaacg agatatgcgg ctatgacttc catgacggca caagctcaaa gcttgaagtg
     2581 cctgacttca ctgcaaagat aggtgagtca atgaagggca tgaagatagc actccctaag
     2641 gagttctatg cagagggcat caatgatgag gtaaaggctg ccgttttaaa ggctgctgag
     2701 gaatataagg caatgggtgc tgagcttatc gagtgttcta tgccgtcgct taagtatgct
     2761 gttccttgtt attatcttct tgcctgtgct gaggctgctt caaacctgtc aagatatgac
     2821 ggtatcaagt atggtcacag aacgctcaca gccgacagct atgaggagct tataatcaag
     2881 tcacgttcag agggcttcgg cgaggaagta aagagaagga tacttcttgg aaactactgc
     2941 ctgtcaagcg gatattatga cgcttactac aggaaggcaa tggctctcag acagcttata
     3001 agaaaagaat acaacgatat attcgagaag tgcgacgtta ttcttacgcc gacaactcct
     3061 gctgttgcat acagccctga gatgacttcc gaccctgttc agatgtatca ggctgatata
     3121 tgcacagtta cagtaaatat cgcaggtctg cctgcaatat caactccttg cggctatgac
     3181 agcaagggta tgcctatcgg tatgtctgtt ataggcagaa agttcgacga ggcaactatc
     3241 atcaagactg ccgacgcata cgaaaagggc ttcaagcctg ttgcgccaaa gctttaaggg
     3301 aggtaagaca aatggaaaaa tggagaacgg tcatcggcct tgagatccac gccgagctgc
     3361 ttaccaaaac taagatcttt tgtaactgct ccgctgagtt cggaggtgcg ccaaatacaa
     3421 gatgctgccc tgtatgtaca ggtatgcctg gcactctgcc tatcatcaat cagcaggctg
     3481 tagagtatgc tgtaaaggca ggatatgcgc tcaactgcgg tatcaacaag ttttcagtat
     3541 tcgacagaaa gaactacttc tatcctgacc tgccaaaggc atatcagata tcacagcttt
     3601 acagacctat cgtaggtcct ggatatgtgc ctatcgaggt gaacggtcag aagaagaaca
     3661 tcagaataaa ccatatacac cttgaggaag acgccggtaa gctcgttcat gacgctttca
     3721 acggaatctc aatggctgac tataacagat gcggtatccc gcttatcgag atagttactg
     3781 agcctgatat agcgtcagca gaagaagcta tggctttcgt tgagcagata tctctgcttt
     3841 tgcagtatgc aggcgtttgc gactgtaaaa tggagcaagg ctcactgaga tgcgacgtta
     3901 atatctctct tatgcgtcca gacgctactg agttcggtac tcgtgcggag ataaagaaca
     3961 tcaactctat caagtctgtt ggcagagcta tcaagtatga ggaaaaaaga caggcgcttc
     4021 ttcttgaagc tggcaaaaag gtcgttcagg aaacaagacg ttacaacgca aacagagata
     4081 agacaacttc aatgcgtaca aaggaaaacg ctaacgatta tcgttatttc ccagagcctg
     4141 atattcttca ggtaaacctt acagacgctc agcttgatga gataaaggct aagctgcctg
     4201 aaatgccaag cagcagattc aagagataca ctgaggaata cggtatttct gagatagacg
     4261 caaaaacgct tatccaaaca aagataatct cagatttctt tgagaatgca cttaagagat
     4321 ataacaatcc taagagcgtg gctgtgttca ttctcggcga gtttatgaga agagtaaatc
     4381 tgggcgagat agatataaac aatatcagct tcacacctga ggagtttgca gagcttgttg
     4441 aaatggctga tacagagaag gtatcaaaga acgacgctaa gaccgtgttc agagcaatgg
     4501 tagaagaggg cggcaagcct atggacattg caaagagcaa gggtatgata atcactgtag
     4561 atactgcaaa ggttgaggca ggcgttgacg agatactcgc tgcaaatgcc gcacaggtag
     4621 agcagtataa gaacggtgaa acaaaggtat tcggctttat catgggtcag tgtacaaagg
     4681 ctctcaaggg cgttgcaaca cctaagataa tcaaggaaat tcttgaaagc aagctgaaag
     4741 ctgctgctgc aagcgctgct tctgacgcag agaagaaaga ggacgttaag gacaacgtta
     4801 tagatacttc aaagctcaca aagtatgaga atgctgataa gtatgttcct gaaaatgacg
     4861 gcaaaatgct tatgatagat acggctgacg tcaagaaaga gtttatgctt gctgacgcta
     4921 aggctaacat gggcaaggaa gttgagtttt caggctgtgt tcatagaata aagaacatgg
     4981 gaagcattgc gtttatcgtt gtaagaacct caagagatgt tatacagaca gtttactctg
     5041 ccgacaactg caaggattct atcgagggtc tgagagaggg cttctttgta aacgtaaagg
     5101 gtatcgtcaa ggaaaaccct aaggatttca atggtcttga aatagaactt gattctatca
     5161 agcttatctc aacgaacgcc gctgagcttc ctttaaaaat atcacagggc agacttaact
     5221 gcacaataga agtaaatctt gacaaccgtg ccgcatcact gagaaaccct tatgagagag
     5281 ctatattcaa gctccaggag ggtcttgttc agggtatgca caagttcatg caggcaaaca
     5341 acttcactga gatacactcg ccaaagatag ttgctcaggg tgctgagggc ggtgctaaca
     5401 tcttccgcct tgactatttc ggcaagtctg cgttccttaa ccagtcaccg cagttctata
     5461 agcaaatggc tgtggcattc tttgacagag tttatgagat agctcctgtt tacagagctg
     5521 aaaagcacgc tacttcacgt catatcaacg agtatatcgg tcttgatttt gaaatgggct
     5581 atatcgacag tatgtatgac gttatgaaaa tggagatcgc aatgctcaag tccattttcg
     5641 agtatatcaa ggagaactat cagaacgagt tgaagatact tgaggctgac gttcctgaga
     5701 taaaggaagt tccgtctatc aagttcgctg acgctatcga gctgctgaga ggcggcgagg
     5761 gcagcggcaa gaagttcgat cttgatccgg aggacgaggt aaacctcggc aagtatgcca
     5821 aggaaaagta cgattcagac tttatctttg ttacacactt cccatcatcg aagcctccgt
     5881 tctatgcaat gaactctagg gaagacccaa gagaagcata caagttcgat ctgttgttca
     5941 gaggacttga aattacttcg ggcggtcaga gaatacacga ttataacgag caggtagaga
     6001 agatgaaggc tcagggactt gaccctgctg acttcaagaa ctatcttgag gctcacaagt
     6061 atggccttcc acctcacggt ggacttggta tcggtcttga aagacttctc atgaagcttc
     6121 ttaacaagaa caacattagg gaaacatcac tgttcccaag agatatcaac agacttcttc
     6181 catagtctgc aaatatgttt atcaaagaac gggcagggtt gtccgttctt tttttatgat
     6241 aaggcaaaaa ggcggtacgt cggggctgta taaaagcaga atatttgtcc aataagaata
     6301 gtataattca atgatactga ttcggaggac tgatattctt gaaaaaaatt cttactttac
     6361 ttatttgttt tttgaccctt tgctcttgcg gaggtgaggt aaattcccag agggacaggc
     6421 ttgtgactat ggaaaaagca tcggtgatac ctgtgtgcgg agagaaaaac tacactgatg
     6481 cggaaacgtc acacaagctt aattatactg tgcagaaagc tatatggata tcctatattg
     6541 accttgctga tatgctcacc gggaaaagtg ccgaggagtt tcgtgagaac ttttctcagg
     6601 cgtgtgaaaa tgctttgagc ataggttgta atacgctgta tgttcacgtc agacctttcg
     6661 gggacgctat atatgattcg gaactttatc ctgcatcaag atatataaca ggcgtggcag
     6721 gggagcagag tgacttcgac ccgcttgata taatgataca gaccgcacat gataaggggc
     6781 tttcttttca cgcatggata aatcctctca ggtgtgagaa tgaggagtgt tttcagaatt
     6841 atgacgatag ctttcttctg aaaaagtggt atgacgaggg cgacggctac ttagaacagg
     6901 ttgagggcga cagccatttg tggctcgacc ctgcttatga tgaggtaaga cagcttatcg
     6961 cagagggtgc ggcggagctt gcagaaaatt atgatattga cggtatacat tttgacgatt
     7021 atttttatcc cacaacggct gaggactttg acgcacagtg cttttctgcc cagacggagt
     7081 ttgacgacct ttctcagtgg cggctgaaca atatctccct tatggtgggg gagatataca
     7141 gtgcggtaaa agcagtggat aaggatattc tggttggagt ttcgcctcag ggaaatatgg
     7201 aaaataacta tgagtatatg tacgctgacg tgaaaaagtg gtgcagcgag gaggggtata
     7261 tagactatat ctgtccgcag atatacttcg gttatgaaaa ttctgtcaag ccttatgagg
     7321 ataccctaca gcagtgggct gatatctgca caactgatgt tatgctggtg gcaggtctgg
     7381 gagtatataa gatagacagc gagagtgaat tttctgaaaa tatcggcatt atcggcaggc
     7441 agataaatga cgctgtagtt agttataact acgcaggagc ggcggtatac ggctatgcaa
     7501 gcttgtttgg tggcagtgat agaggtgttc aggagcagga gtttatcgaa attgcttttg
     7561 ataaacttga tacatctgta aaatgatatg cgatatataa attatggtat gttaaatgta
     7621 aacaaattgt gatatattat tataaaattt atggaagtaa acaaaaaact attgaaattt
     7681 ctttttaaat atagtataat gttata
//
LOCUS       DAWEMR010000124         7657 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3935_length_7657_cov_10.392660, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000124 DAWEMR010000000
VERSION     DAWEMR010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3935_length_7657_cov_10.392660"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2216 a         1692 c         1558 g         2191 t
ORIGIN      
        1 cactggaaga aactcgttga aaaataccct atcatctcta tcgaggacgc tctcgacgag
       61 gaggactggg agggctggca gcttctcact aaggagcttg gcggcaaggt gcagctcgtt
      121 ggcgacgatc tgttcgtaac aaatacagaa agacttgcaa agggcatcag ccttggctgc
      181 ggcaactcta tccttatcaa gctcaatcag ataggctcag tttctgaaac acttgaagct
      241 atcaaaatgg ctcataaggc aggctacact gcgatttctt cacaccgttc aggtgaaaca
      301 gcagacacaa ctatcgctga ccttgcagtt gctcttaaca cttgccagat aaagacaggc
      361 gcaccaagca gaagtgagcg tgttgcaaag tacaatcagc ttctcagaat tgaggaagaa
      421 cttggcgaaa gcgctgttta tccacaaatg ggtgcattta acgtaaccag atagtttttt
      481 gtcatataag gatcaagcta caagcttgaa aaaaagcagt ccacaggggc tgcttttttt
      541 gttgtataaa tgcagtgtag gggcgacttg aggtcgcccg cttatcatca tctgtaagta
      601 gcatcagaca taaaaaaatc acgccctaac aaagtaaggc gtgatcttga ctaaggatat
      661 ttccctagtg gtgagctatc ggggattcga accccggacc ctttgattaa aagtcaaatg
      721 ctctgccaac tgagctaata actcatatcg tcctgcaacg acttaattat tataccaatt
      781 ttccacccat ttgtcaaggg gttttcgaga attttttcac tttttccaaa ctgcacatat
      841 ttatccccca tttggcatac ttttatgcgt tttctaacaa aaaaacgccc ctgaaaatca
      901 agggcgtttt atctgcaaaa ctttaagatt agtttatctt gttgccgcag ttagggcaga
      961 actgatttcc agccggaact ttacctccgc aagtagggca gaacttttca ccctgtggtg
     1021 cggcctgagc ctgtggctgt gcatatgtct gctgtggagc gttctgctgt ggaacagaac
     1081 ctgcatactt cttgaaaagt gcatagatgc cgcagataac aagaagaaca aatgctatcc
     1141 agaacagtac atacatgaat gtgtagaaac cgccatacag cttgctcata cctgaaagat
     1201 agcttcctgc tgcatatgta actgcaagac ccagaacaac acctgcacca gtagttaccg
     1261 gcttgaggta cttgtcagca attttgaaaa aatcgttcat tagagaatgc ctcctaattt
     1321 aataatatat atttattata caatcgttat gtcataaagt caatagattc gagtaaaatt
     1381 tttcacttgt ttacgtttct atttgtttgc tgtaatgaca gaaataagca taaaattgct
     1441 attttcttta ccctctgtgt caataactat ctcaacagtg tgttctccct ttgctccgct
     1501 gtatatctcc tcaagacagc aaaggtctcc ccaatcctcg tcaaagtttg cgtcaagaag
     1561 aactttgttc tgcctgtcac cgtctatgac agcatatgca acaggcgcag gcttgttgat
     1621 agtctttctg tattgcagca ttataatact tccactcacg ttgaatttaa tacaacttcc
     1681 ctgcttgtga gctatccaac cgcccttgaa tggatcgctc cactgctctg ccgcatgagt
     1741 atcaggctca aagccctcca taacgggcga acagctatag ttctgaaaac gctgtgcgtc
     1801 ctcatatctg cttgctgtaa atggctcagg aagtgaaggc ttcttctctg cgccgagctt
     1861 cttatagtat gaatattctg tatcaagcag attgcagata agatcagcta tcatcttgtg
     1921 acccaggtct gtagggtgga gcatatccgc tgtgtagtct gcaagacaaa cgtttccaag
     1981 ctgtatctca gggaaaatgt agtttcttac actgacgata ggcaggtcat aatgcagacc
     2041 gatagcgtta tgtataccct gtgcatttgt gcctgtattg taaaaaagat tgtttataag
     2101 cacaaccgcc ttgacgctct cagctttcag cagacgtctt acaaggctct cgtatgtttc
     2161 cataaatagg ggcgaactgc tgtcgtttac cgaaaactca acgaaaacaa ggtctggctg
     2221 tttgtcaagg acgtgttcct gcactcttgc caccccaaac tgcgaggtag ttgcaccaat
     2281 accggcgttg aagtagctta tctcagcgtc aggaaaattc gtctgccacc attttgcact
     2341 gcggtcaaca tatcttaact cagtttttgt agcattacag cccattgtta tcgagccacc
     2401 caaataaaca atgttaagct ttttttcatt ctctatcttt ttgaatacct gtggaagata
     2461 acccatactg ccgatattca tgatagatac gtttttgtcc gtcatatcca tttctccttt
     2521 tatttctata atggtctatt atagtattat agcatttttc aaaacaaaag taaatcataa
     2581 cgggcaaaaa tattttgctt taaactacaa aaagcggtaa tgttttttgt taaaatcgtg
     2641 caatgtagat gttgacgaaa agtaatgata tgttgtataa taaagaataa atataaaata
     2701 tgaagaaaga agtgttcata atgcacgatt ttaaggcact ctgccgtcag gcacacacat
     2761 tagtaatatt caagagctta catgaagttc ctgtctttga aaagctactt gacacacttg
     2821 ctgtatgcga atctgacagc gacatggcta tagaaaaata ctcagatttt gtggctgagc
     2881 tttttgcata ttcggataat cttacagaat atatgctcaa gctggttctt gaaaatgaaa
     2941 acctttttat gcttaaaaag ggagaaggca aggagacagg tgcgcttttg gaggaatgtc
     3001 ttgcaaacga gcttgccgtt atcgaggagc tttcacagat accaagtgac gagataatct
     3061 caaagataga ctatgacggc tttctgccga gatatgctac acaaaagctt gacttctcgc
     3121 agatatatgc cgaccgtata cacgctatcg gtcagtacgg ttacggcatc tattctcagt
     3181 atcatgtatt tgtcataaaa gacggcaaga tagttccggt ggagtatcct gacgatataa
     3241 agctttccga cctgcacaat tacgaacgtg agcgtcagga ggtcatcaac aatactcttg
     3301 cgcttttgaa aggcaagcct gccaacaacg ttctgcttta cggtgactgc ggtacaggca
     3361 agtcctcaac agtcaaggca atagccaatg agtatgcctg caagggtctc agacttatcg
     3421 agcttaaaaa gaagcagctc catgagatac cagccattgt agaaacggta agcaaaaatc
     3481 cattgaagtt cattatcttc atagacgacc tttcattcac agaggacgat gatgattttg
     3541 ccgcactgaa agctatcctc gaaggaagcg tttcatcaac tgcggacaat ctctgcattt
     3601 atgctacctc aaacagaaga catcttgtac acgagacgtt ctcctcacga gagggtgacg
     3661 agatacactt caaagacact atgcaggagc ttctttcact gtcagaccgt tttggactta
     3721 ctgtcacata tcagaagcct gacaagaaga tctaccttga tgtagttgag aaacttgcaa
     3781 agcaatacga gcttaaaact cctattgatg aggtgcttca aaaggctgag gcttatgcgc
     3841 tttcgagagc aggacgttca ccgagagttg caaagcagtt cgtggaatac atgaagggca
     3901 tggaagacta ataaatatat tctgacgata ttcaggagga attatcatga caaagcagcc
     3961 acagtggctt gacaacgcag ttttctacga gatctatcca caatcgttca atgacacaaa
     4021 ctcagatggt atcggtgata tcaagggtat tacagaaaag cttgactata tctgcgagct
     4081 ggggtgtaac gctatttgga taaacccatg cttcaaatca ccttttggcg atgcaggcta
     4141 tgacgttgag gactattaca cagtcgctcc acgttatggc actaacgagg acctggtgga
     4201 gctttttaat gaagctcaca agcgtggcat gcacgtcctc ctcgaccttg tacccggtca
     4261 tactgccgtt actcacaagt ggtttaagga atcaatgaaa gccgagagga acgagttcac
     4321 agaccgctat gtgtggacag acagcatatg ggtagagcct gacggcatgg ctagtatacg
     4381 tggtatctcc gacagggacg gctcatgtgc cgtgaatttc ttctcacatc agcctgcact
     4441 taactacgga tattataagc ctgatcctga taaaaagtgg cagcagtcta tgaatgacga
     4501 gggtccgaga gccacacttg aggctctcaa ggatataatg cgcttctggc tcagtgccgg
     4561 tgcagacggt ttcagagtgg atatggcagg ctctcttgta aagaatgacg agaacggcaa
     4621 gggcactatc gcactttggc aaaacgtaag agaatttctt gataaggagt tcccatatgc
     4681 cgctatggta tcagagtggg gagaacctga caagtcaata atcggcggtt tccatatgga
     4741 ctttctgctg cacttcggtc cgtcacatta taatgacctt ttccgctgtg aagagcctta
     4801 tttctcaaag cgtggcaagg gttcggctaa agaatttgtt gaagcataca aaaccaatta
     4861 tgccaagaca gacggcaagg gacttatctg tatcccgtca ggcaaccacg atatggacag
     4921 acttgcaaga acccttgata cagacgaaat gcgtgttgca tttgctttcc tgctcacaat
     4981 gccgggcgca ccgttcatct actatggtga cgaaataggc atgagatatg tggagaatct
     5041 gacatcagtt gagggcggct atggcagaac tggctcacgt tcgcctatgc agtggaacaa
     5101 ggggcttaat gctggcttct catcagctaa ggctgaaatg ctctatgtac cgctggacag
     5161 cagtaaagac agacctgacg ctgaaagcca attcaaggac agcacatcac ttagaagcga
     5221 agtcaaaggg cttataagct tcaggcagaa aaatcctgct ctgcaaagca ggggcgagat
     5281 agagtttttg tcaagcggat atcctctggt atacagacgt aagggcgagg gacagagcat
     5341 actggcggtc ataaatccaa aagacgaaac gacagagatc aaaaacatca atggcaagat
     5401 aatttataca gtaggtcatg gcgcaagcca tgacaacgga aagctcatta gtaaaggctc
     5461 ggccgctgta tttgttgaag aataaaataa cataaaagag gtcagggaca cattccttga
     5521 cctcttttgt tatgccgtct aaagtttatc caccgttgtc agagcatact ccgaaactcc
     5581 gttggagttt ttccaaagtg cattttaaac cgcttcataa aaccatattc attagtataa
     5641 ccgcacctct cagccacttc ccttacgctg agatttgtcg ttgcaagaag ctgactggct
     5701 ctttccattc gtgcgcttat aacgtctgtc atgacgctca ctccaaacat tttcttatac
     5761 atatgctgaa aacttgacct gcttataccc atatcccatg ccatgctacc tacgtctgca
     5821 atatcctcag gcattgcgta tatgtatgct ctgagctgca taaagcggct gttcctgtca
     5881 gtaataatac aggacttctt gtctgtacac tttagctgtc ttgcaagctt taacaaaaag
     5941 atctccatat agcaccgtgt tacttcctca tgcccctctt tcatgagctg atactcatat
     6001 gctaccgaac gcattatctg actaagctca tcgatatttc caaggtatac cagtgtatct
     6061 tttggaacat caagcttttc aaatttttca ttatcgccct gctcataatt gaaatacagc
     6121 caatcatcgg tataaaactc ttctgcggca tggtatttca caggggtatc cggtgaaaga
     6181 aggataaaag aatttttctc tgccctatgc tcaataccat ttataacgaa caccgatggt
     6241 tcttttatga gcaatataag aggacagccc accccgtgag ggcaatccat aataaaatct
     6301 tttctatggc gataattata gccgaccgca cccacaagca ttctgattcc tccctgcttt
     6361 tatcaacttt attttaccac accgctacat tcttgtcaac gaaaaaagag cagacgcttt
     6421 gttctgctct tctttcacat aattctataa tagtattaca attatcccca ataggttctc
     6481 caaacattga agtaacccca aggaatttta aatgagtaag ctatcttgct gctgtttgtc
     6541 ttgtagcttc tgttgcacca gaagtaaaca ttatagtcat tagcaatacc gttaaggtga
     6601 gttttgccat atactgcacc aaaaacagcc tgctttactc ttctggaaac gtttgaataa
     6661 tttgctccgt cccttgagag ctgtgcagat gacataaatc caccgctcct ataaataata
     6721 tcctgaacat tgttatatct tgcatagtag gatctccaca ttgcattctt tgtgtacttt
     6781 gcggcaacat actggtttgc aacacaatct gcaacataaa cgataggctt atcccactcc
     6841 gaacctgaca tcttaccaac ctcatgataa actatacggc acattgcttc ccagcctgcc
     6901 ttgttgaagt ccattctggc tgtctttatt gtctgtgtct ttgagtaagc agaagcggca
     6961 gagccattta cagcctttac tctgaactga tatgaagtcg ttctctgaag acctgtaaca
     7021 gtgcagttcg tgttcttgac tgtcttgtat ttcttccaac tcttatattt gccacccttg
     7081 atgtatagct catattttga agcacctttt accttgctcc aatgcacctt gatagtgttt
     7141 gtctgcttgt tatacggatg agtgaactca cttccatagt ttatctgagc tgtcatctta
     7201 ggagtcgaaa gctttgccgc agcagcatcg gcactaaatc cctctgtcat taccaaaaat
     7261 gtcataagtg ctgcaataaa catcactata actttcagtg acttattctt tgttgaaata
     7321 aaacttgctg aatgatccat taataatacg gctttacaac gcatacacgc tgcattgcca
     7381 attcacaccc tttcttttaa ttagcggcgg tcatgtctgt gccgctattt aattttatta
     7441 cggtcgatta aaattatgta tcaatctgta acgataacgg ttaagaaagt tacaaactga
     7501 ttacaaatta ctacactatt gtaacacgca caattgcatt tgtcaagctt ttttatagtt
     7561 tttttgattt ttgtttataa tttgaagcaa agaatataaa aattgtacta atatttcagc
     7621 acattgaata ttaatttact gtatcagcaa gagttca
//
LOCUS       DAWEMR010000125         7636 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_3948_length_7636_cov_8.687904, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000125 DAWEMR010000000
VERSION     DAWEMR010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3948_length_7636_cov_8.687904"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3029..3038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3602..3611
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6377..6386
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2231 a         1393 c         1857 g         2125 t
ORIGIN      
        1 atatgctgta aggcgataat agaaaatgtt tttgtattat ttgcctaaaa caatattatt
       61 ttgctaatta ttcagggagg gattttagtg tacaaaatcg taacaagaag acagctcaat
      121 gacgctgttg ttcttatgga gatcgacgca ccttacattg ccgctaaggc tgaggctggt
      181 cagttcatca tctacagagt tgatgagaag ggcgagcgtg ttccgcttac tatcgctgac
      241 tatgacagag aaaagggcac gatcacaatt attttccagg tagtaggtgc atctacaact
      301 gagcttgcaa gacttaatga gggcgactat atccatgacg ttgttggtcc tctcggcgtt
      361 gctacagagt atgacgaggg tatcaagaga gttgcagtag taggcggagg cgttggctgt
      421 gctatcgctt accctcaggc taagaaactc cacagcatgg gcgttgaagt tgatcttatc
      481 gcaggcttca gaagcaagga tatcgttatc ctcgaggacg aaatgggcaa ggcttcttca
      541 catcttcata tctgtacaga cgatggttca tacggctatc acggatttgt tacaaacaag
      601 cttcaggaac ttattgacgg tggtgctaag tttgacgagg tcatcgctat cggaccagtt
      661 ccaatgatga agttcgtttg caaggtaaca aagccttatg atatcaagac tctcgtttca
      721 ctcaacccta tcatgatcga cggcacagga atgtgcggcg gatgtcgtgt aactgttgac
      781 ggcaagatca agtatgcttg cgttgacggt cctgactttg acggtcagaa ggtagatttc
      841 gacgagctta tgagaagaaa ctcaacttat aaggagcagg aaaaggctca cttgtgcaga
      901 cttactggag gtgtaagata atggctaata tgagtttgac aaagactcct atcgcagagc
      961 aggagccaaa ggtaagagct agaaacttca aggaagttac tttgggttac acccctgagc
     1021 aggctataga agaagctcag agatgtctta actgtaagca caagccttgt atgacaggat
     1081 gtcctgtaag cgttaggatc cctgagttta tcgcaaaggt agcagagggc aagtttgaag
     1141 aagcttatgc tatcatcaag acaacaaacg ctctgccggc tgtttgcgga cgtgtatgtc
     1201 cacaggagca tcagtgtgag ggcaagtgtg taagaggtat caagggtgag cctgttggta
     1261 taggcagact tgagagattc tgtgcagact atatgatgga caaggcagtt gctgtagaaa
     1321 aggctgagcc aaacggtcat aaggtagctg ttgtcggcgg cggtccttca agccttacag
     1381 ttgcaggcga ccttgctaag cttggttatg acgtaactat ctacgaggca ttccatacag
     1441 caggcggcgt tcttatgtat ggtatccctg agttcagact tccaaaggct atcgttcagc
     1501 acgagataga caatctgaca aacatgggcg tacatatcat gacaaacatg gttatcggca
     1561 gagttctttc tatcgacgag cttatgggca tgggctatga agctgtattc gtaggctcag
     1621 gcgcaggtct tccaagattt atgggtatcg agggtgaagg tcttgtaggc gtttactctg
     1681 ctaacgagta ccttacaaga atcaacctta tgaaggcata caaggacgat tatgataccc
     1741 ctatcatgaa gtcaaagaac gtagctgtag taggtggcgg taacgttgct atggacgctg
     1801 ctcgttctgc aatgagactt ggcgctgagc acgtttatat cgtatacaga cgttcaatgg
     1861 acgagcttcc tgcaagaaag gaagaggttc atcacgctca ggaagagggc atcgagttcc
     1921 tcgttcttaa caaccctgtt aagatagttg gcgacgagaa cggtcatgta aacggcatgg
     1981 agtgcatcaa aatggagctt ggcgagcctg acgcaagcgg cagaagacgt cctatcccta
     2041 tcgagggttc aaacttcatt cttgacgttg atacagctat catgtctatc ggtacatcac
     2101 ctaacccact tatcagaagt acaactcctg gtcttgaaac aaacgatcac ggctgcctta
     2161 tcgttgacga gaacgagtca acaactaagg aaggcgttta cgcaggcggt gacgctgtta
     2221 caggcgctgc tactgttatt ctcgctatgg gtgcaggcaa aaaggctgcc gctgctatcg
     2281 acgagtatat caagaacaag aaataatttc ttgtaaatga aaattatttg ttaaaaggct
     2341 tgaagtttgt gcaaatatct gatatactaa tatagtatta ttagatttgg aggtcttttg
     2401 atgaatttgt cagcaattat ttcagttttg gcagctgagc agagttcacc tgcatcactt
     2461 gccatgactt ttatcccact ggtactcata atcgtgttct tctatttctt tattatccgt
     2521 ccgcagaaaa agcaggataa gaaagacgct gagatgagag ataaccttga gataggtgac
     2581 gaggtcgtta caaacggcgg aatcgttggt atcgtgttct ccatcaagga cgacactgtt
     2641 gttatcgaaa caggcggcga cagaagtaag atccgtgtta aaaagtgggc tatcgctaag
     2701 aacgaaacaa ttcatgacgg tgacgtaaag taaatattgc ttttgaggga cgggtagttt
     2761 tatccgtccc tttttgtgtt cttattattc gtggctgtga atataatttt tctagggaat
     2821 tttgtatgcg ggggtgcgga tatgaaaaac aacagactat ttgggggcag aaagctttct
     2881 gtgctatcat ctacagcaat ttctgcctgc gtggggtgcg gtgcgatatt tgttgctgtg
     2941 cttgcttttg cgtatgttat cactaagata gatgccaccg atcttatgct gtcggtgatg
     3001 tcaacagcgg cacttggtat cggggcgtnn nnnnnnnnac gtagaaacgg tctgctgtca
     3061 ggtgtgctgt gcggagtgtt catttttctt atcattgtca ttctcagtgc actgttttca
     3121 aaagcagccg aaagcttttc cgtgcctgca aagcttgtgc ttacccttgt gtgtgcaggc
     3181 gtaggcggag ttgtaggggt aaattctaaa agcagccgtt tttgataagg ataaatattt
     3241 atactgcatt aactattcgt tcataaaaca tagcgggata tgtggtataa ttagtgtaaa
     3301 tcaattattg gaggtaaaat cctatgaaac atatcaagac gatcaataag gctaatctca
     3361 agaagactgc tgctaagggt ggctgcggca agtgtcaggc atcatgtcag tcagcttgca
     3421 agacctcctg cacagttgct aaccagaagt gcgagagaga agcttaatat tccgatcttt
     3481 ctgaggcgtg cgagcgtaca cacgccttgc taaagggaca tttttatgtc cctttatttt
     3541 tgtataaatt tgtatatgaa tataaatgtg aaaggtgatt tttaaatgat acacaagttt
     3601 annnnnnnnn ngcggcttta atgcactgct tgacgtgaac agtggcggag tacatattat
     3661 agacgatctg acatatgatt tgttagacaa tgttgagccg ccttttgagg agaaatgtcc
     3721 tgaggacgtt gtaagaaagc tgtcaaagat gtatgacagc aaggatatcg aggattgcta
     3781 tgaggagata gtttctcttt acaatgacaa gatattgttc agcgaggacg attacgagaa
     3841 gtatgccctg tctgctgtgg cttcacctat caaggcaatg tgtctgcata tcgctcacga
     3901 ctgtaacctg agatgtaaat attgctttgc ttcaacaggc gatttcggtc agggaagaat
     3961 gctcatggac tatgaaacag gcaagcgtgc tatagatttt cttatagagc gttcaggaga
     4021 cagaaaattt cttgaggtgg acttcttcgg cggtgagccg tcactgaatt tcggtacagt
     4081 ggaaaagctt gttgagtatg cacgttcaca ggaggagcct cacaataaaa agttcagatt
     4141 tactgttacc acaaacggtg tacacctcac tgacgagatg atagatttca tcaacaagga
     4201 aatgtacaac gttgttctgt ctatcgacgg aagaaaagaa gtcaatgaca gaatgagagt
     4261 gcgtgttgac ggttcgggta gctatgacag gattatccct aacttcaaga agcttgttga
     4321 gaagcgtcct aagaacaagg attggtatgt aagaggcact tacacaaagt ataatcttga
     4381 cttctcaaat gatgtaatgc acctttatga ccttggattc gaccagatat ccgttgagcc
     4441 tgttatggct gaccctagcg agccttatgc tatcactgag gctgacctgc cgaggatctt
     4501 caaggagtat gagatacttt ctgagaagat acagaagata agggacgagg gcaagttcat
     4561 aaacttcttc cactttatga tagacctcga tcagggtcct tgtgcgataa agagacttag
     4621 gggctgtgga agcggaaacg agtatgtttc tgttactcct gacggcgata tttatccatg
     4681 tcatcagttc gttggtatca ccgagttcaa aatgggcaat ctctatgacg gcacatttaa
     4741 cgaggaaatg aaagatatgt ttgctcacgc acacgtttac aacaagccag agtgcaaaaa
     4801 gtgctgggca aagttctatt gcagtggcgg ttgcaatgca aacaactaca tttacgcagg
     4861 tgatatccac aatgcgcata agctgtcatg tcagatacag aagaagcgtc ttgaatgtgc
     4921 tatcatgatg aaggctgtta aaatgctcga cagcgcagaa taattattaa tattttgtaa
     4981 atcttgctta tgataacttt tgtgtacata tataaactta atatgtacaa aaacgtttga
     5041 agtttaacat aaaaatttat tgaataatcg gtttaaaaag cacggaataa caaatgacgt
     5101 tatttcgtgc tttctgctgt tattacatag tatgatcatg tcggaaaata gcaaaatgtg
     5161 tctgtcatat taataagtat taagataaat ataatgtata ctaagctggt agcttattga
     5221 caaagtgggt cgaatttagc ataatattga cataaacaaa aggtgcttgg cggtcatgtt
     5281 accgcaaatc ggctataacg agcgccctct gtatcggaca ggataaaacg acaaattatt
     5341 atgaaaagga cgaagagata tgaaaactaa tgaaaagaag aatacttctg ctaagaagaa
     5401 acttatacct gcagttgcaa tgctcacaac ttccgctgta atgctttcta ctgctactta
     5461 tgcttggttt actatgagta gagaagttga agtaacagga cttcagatgt cggcaacagc
     5521 cagtgatgca cttgaaattt cactgggtga agttgctaag ggcacaaaca caattggtgc
     5581 agatttagca aaacagccag cagatactga tgcagaaata agctggacaa agagaataga
     5641 tataagtgag tattattcta ctgttgattt acttatgcct gcatctacgg tcaatggcaa
     5701 agatttcttt gatgcaactg atgcaacaaa cgcaggtaaa tctgcaagta cgtttgttgc
     5761 cgcaacaaag aaagcagaaa ttgacaagag agatgcaaag acgacaaagt ctaatcttgc
     5821 tactgataag gataacagcg gctattatgt ggacgttcct gttcatctta gaacctccaa
     5881 atatagctca acagcagaaa acaacacaat atattgcaag atgagcatta cagatcctaa
     5941 gggaactgga gtaactgatg gcgatttgta caaagcggtt agggttgcat ttattcctgc
     6001 tgacggaagc gccgcatcaa tttggtgcgt tgatgattca aattatgaaa caggaaaagc
     6061 agtaagtagc acaacagcaa agacagctat tgctgcaggt cttgtgaagt ctggagatgc
     6121 tcttacttct ggtgagtctg atattggaat aacaattcct tcttcgacaa ctgcaggtaa
     6181 ctatggtcat gctgatttta ctgttcgtgt atggcttgag ggtgaaagtg agtattgcca
     6241 tgatgcgaat gctgcacagg attggaatat tgattttgca tttgcactta ataaaacaaa
     6301 tcaagatttt gtcaatttcg gaaaaacaac ggttggcgaa taatccacaa caataaaaca
     6361 aaaaagacgc cgaagannnn nnnnnntttt ggtaaatggg tataaaaaat aattttccat
     6421 atttggggaa atctcccgat ttttctttgt tttttgtcga aaatatgcta taataataca
     6481 ttggagcaga aaggaagtgg ctttgctttg ggcgaaaatt ctacagttga cggcgttcgt
     6541 gccatggagc ttttctatag acctataaaa aataccgaca gcggcagggt cacttttttg
     6601 cagtcgcaaa caaggctcaa ttctcctgtt atgggaacgc taatgcctgc ggattatctc
     6661 cctgtggctc agctttccga ccgatgtgtt agcattttca agcttgcttt tgttcagctt
     6721 ttgcaggctt tgggcaagtt tcacgaaagg gaagttgacg ttgattgggc aagcgtgttc
     6781 atgcctgcaa gatttttaag gcagacggaa gctccgagaa ttgcggcaga ttattgcgat
     6841 aagtttggcg tgaagtatga gcgtgtctgc tttgagttgt cgcctgatgt gcttgatgaa
     6901 gctgacagca cagtggcaga taacgtggct cttatgagaa gacagggctt tcattttctg
     6961 ctcacaggct tcggcactga gggctttaaa atgcttaaga ccgctgagtt caaggtagat
     7021 aatatcatgc tttacggctc agtggctcag gttcttggta agagcgacag gtatgatacc
     7081 tgcgtgaact ctctgctgaa tttcgttttt gaccttaatg ctgaggtcat agctgacggc
     7141 attactgatg ataagcaggt gaaacagctc agcggtatgc agtgccttta ctattcggga
     7201 gaaaatgcag gaaaatatat tgcggaaaga tacgttcgca atagaaatga gtaataaacg
     7261 ggtgatacgg ttttggacgg taaaaatata gataaaaata caagagtagt cgataccctt
     7321 gcacttcgca ggaccgccaa ggaagctaaa cgctatgtgg tattgatgag attgcttaaa
     7381 atacttgcca ttatacttat cgctatcgtg gcggcggctt atgcggtgtc ttatttttac
     7441 gacaagtatg gtagctttac agttaagata agtaagtatg atatgataaa tcaggggctt
     7501 actctttcgg aaactcctga ctataccacg agcaattcaa ggctcaatgc ggatatcctc
     7561 tatgatatga caaatatcag tggcgaagat ctccctgata atatcgacaa gataaacggc
     7621 agccataatg gcgagg
//
LOCUS       DAWEMR010000126         7500 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4036_length_7500_cov_10.309201, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000126 DAWEMR010000000
VERSION     DAWEMR010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4036_length_7500_cov_10.309201"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3778..3787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2319 a         1309 c         1725 g         2137 t
ORIGIN      
        1 ctatctctct tttctttttt cttaattttg tccaatatct attgtagcac tacagaagtt
       61 gaaatagcca tttaaaataa aatgttgact tgtaggcttt ttaatggtat aatataaaaa
      121 atatgtggaa aggatcatat atgatgagag ttgagcttat tacccataca cctgagcctg
      181 aaaaggtggt tgctactgct gcaaaacttt gctattcaag cagtgatatc gcctcgctta
      241 gagagggtct tacggaagaa aaaacaaaga gctttattga aaggctcgtg tctattggtc
      301 acgaaagccc tatggaacac gtttctttta cttttggcat agaggggata tcccgtgcct
      361 gctctcatca gttggtaaga cacagaattg cgtcttattc tcaaaaaagc cagcgttatg
      421 taagtgaaag cggctttgag tttatcacgc caccatcaat agaggctgtg ccagaagcaa
      481 aggctgaatt tgacaagcaa atggatcagc tacttgagtg ttataataag attgccgaca
      541 tacttacaaa tacacatgaa aaggcgtttt tagagcaggg tatgtctgaa aaggacgcac
      601 ttaacaaggc taagaaaaaa gcctatgagg acgcaagatt tgttcttcct aacgcttgtg
      661 aaacaaagat agttgtgaca atgaacgtca gaacactgtt taatttcttc cagcatagat
      721 gctgtaacag agcacaatgg gagataaagg ctgtggctga tgaaatgctc agactttgca
      781 agcaggccgc tcctgagctt ttcaagaacg caggtccgtc atgtgtaatg aaaggcaagt
      841 gtccagaggg cgcaatgagc tgtggaaaaa tggctgaagt aaagaaaatt tacggagaaa
      901 tgaataatgt tgaatagatc tggacttata gttcttgagg gacttgatgg aagtggaaaa
      961 tcaactcagt ttgagaggct ttctgcttat ttcagcgaaa atgacataaa gcataaggct
     1021 ataagctttc ctgactatga taatccgtca tctgcgttgg taaagatgta tcttaatggt
     1081 gagataggca aaacgcctgc tgacgtgaat gcctacgcag cttcaagctt ttacgctgtt
     1141 gacagatacg cttgctacaa acaattttgg gagcatgact ataaagaggg aagtcttgta
     1201 cttgcagcga gatatgttac atcaaactgc atttatcaga tgacaaaact gccgaaagag
     1261 cagtgggaag attttataga ctggcttgaa gattacgagt atgacaagct tgggcttcca
     1321 agacctgaca aggtgatatt cttggatatg ccaatagaga tatctcaaaa gctgatgtcg
     1381 ggaagatacg gcggagatga acgcaaaaag gatatccatg aagcaaatgt ggagtatctg
     1441 aaaaaatgcc gtgaagccgc tttgtttacc gctgagaaac aggattggaa tattattcct
     1501 tgcagcgacg gaagcgagcc ttatacaaaa gataagattt tcggagatgt tctggggtta
     1561 atcaatagaa agtaatgtgt gtttaatgtt tgatttcaga aaaataagtt ttgaggacaa
     1621 agaatggata accaagtgtc ttgcagtatc agatttcaga ggagcagaat attgctttgc
     1681 aaataatatg gcgtggcaaa gacttaatga cactgtaata acacatcatg gtgattttta
     1741 tataagctgt tcctttgagg acggtcagcc gtattttaca tttcctgcgg gcgttaaaat
     1801 agacgttgag ggcaaagaaa aatatatcag gctttttgat gagcttaaag aatatgtttc
     1861 cacccaggga aagccgctta tcgtatcttc tgtaacagat gataacctaa gctggataaa
     1921 agaatattat ggtgataaaa tcatctgcga gtatgatagg gacagttcag actatattta
     1981 caatgcttcc gaccttatcg agcttaaagg caaaaagtat cacggcaagc gtaaccacat
     2041 aaagcgtttc atggacgagc cttgggaata cagaaagctt actgataagg aaatagacag
     2101 ctgtattgaa ttttcggctg agttttataa taagaatgac aacgctgacg atccttcggc
     2161 ggtggttgag caatatgcta ttgatctttt tcttacgaat atggataggc tcggacttaa
     2221 aggcgctgtg ctttacagaa atgataaaat gacaggcttt acggttggcg aacaaataaa
     2281 ttcggatacc tttgtggtgc atatagaaaa agcactcgct gacgttcagg gggcatatcc
     2341 tatgctgtgt agtcagtttg cgtcgcataa tgcaaagggt ctcagcttca tcaacaggga
     2401 agaggatcta ggaatagagg gtcttagaaa gtcaaagctt tcttataatc ctgcttttct
     2461 tttgaataaa aatgtaatta ctttcagatg atcgaaagga gtcatttgtt atggtttatg
     2521 tatttcttgc caatggtttt gaggaaatgg aggctcttgc ccctgtggat ctgctcagac
     2581 gagcaggcgt tgaagttttt actgtaggtg taggctctga tatgatagtc agctcacaca
     2641 acattcctgt caaaaccgat acaacagtgg ataagatagt tcttaatgac gagctcgaaa
     2701 tgatagttct cccaggtggt atgccgggta ctttaaatct tgaagcaagc cctgatgtgc
     2761 ttggtgcagt tgattactgt gctgataata acagatacat tgccgcaata tgtgccgctc
     2821 cgtccataat cggtcataag ggtcttcttg acggcagata tgcaacttgt tttccaggct
     2881 atgagaaaga tcttaaaggt gcgatacatt ctgccagact tgtggctgtt gacggtaagt
     2941 tcatcactgc aaagggcgca ggctgcagca tgaagtttgc actcaagctt gttgagcttc
     3001 ttgtttcaaa agaaaaggca gaagcccttg aatcaggtat gcagagcaaa tgacagataa
     3061 aaaagcttta agaaagcatt tcaaagagat acgtcttagt atgtctgcgg aagaaagaaa
     3121 acaggcagat gtggctgttc agcaagcttt tcttgacagc gaggagtata aaagctgcac
     3181 aactgtgctt gcatatgttt cctctgatat tgaagtgtct acggcggaga tagtaagaaa
     3241 ggcgctaaag agcaagatag tgctatgccc gagatgtgaa aaaaacggca atattatgta
     3301 tttttacaga ataaacagca cctatgatct caaagagggt aggtttggca tttttgagcc
     3361 tgatgaaagt tgtgagaaat actgcgtttt tgacaatgcc ttgtgtatcg tgcctgcgct
     3421 ttcgtatgat aaagacggat acaggcttgg ctttggtatg ggcttttatg acagattttt
     3481 ggctgattat aatggcataa agctaggttt atgttacgat aactgtatgt cagaaagttt
     3541 ttgtcatgac ggctatgata tctctgtaga caaggtattt actgataagg atatatatac
     3601 tataaacaca aaggaaagga agtaaataat ggacgatctg aattttaatg ttgacgatat
     3661 aataaatgat gtcaacgcaa agataaagtc tgccgagaaa aatgaagcgg acgaagaaag
     3721 cgttgaagca gagcctatcg ctgacactga gaacagttcc gaagcctgtg aacaggcnnn
     3781 nnnnnnnacg agaaaccaca ggaaactcca gaagcgaccg atgacaagcc tgaacctatc
     3841 ctagcttttc acgaggacaa gtcagaggaa gacaaaagcg aagaagacga gaataaagag
     3901 ccagagaagc ccgaaaagca agaaaaaaag tcaaaaagcg gcaagaagaa aaagaaaaaa
     3961 agaaagaaaa gcaaggtgaa taattcggta tttggcggaa ttatacttgt tatagtaatt
     4021 cttacagtct ctttagtgct tgcagtgggc ggcatttcta ttggcatgga gtattatggt
     4081 atcggaaaaa ctgacgagga tatcagattt aatatccctg caggttcaac aaacagtgag
     4141 atagctgata tacttgtaaa tgagggagtt ataaagaaca aaaagctgtt tatgcttgct
     4201 ttgaaatttg aaaagcctgc agctatatat cctggtgata tcacacttca gccatcaagc
     4261 ggatattctg aaataatcga aaaactttca caaatgagag agtcatacaa gactgtaagc
     4321 attacattca cagagggaga aaatcttctt gacatagcta aaaagcttga agataatgag
     4381 gtctgcactg ctgacgactt cctgtttgaa ttcaataaaa atcaaggctt tgactttgaa
     4441 aatgatgttg atgataacgg cgatatgttc tatcgcatgg aaggctattt ctatcctgat
     4501 acatatgagt tttatgtgaa tgacagtgca ggaaacgtta ccaagaagct tagagagcag
     4561 tttgaaaaga aatacgaaac tgtcaaagct aagatcaaaa attccggtat gtcacttaac
     4621 gaagttatga cccttgcttc tatcgttcag cttgaagccg cttctgagga cgaaatgcca
     4681 aaggtagctt cggtatttct gaacagactt gacgaccctg acacctatcc tatgctccag
     4741 tctgatacaa caacaaatta tattaagaat gttataaaaa ccgaggctga caacacagct
     4801 tctatcgagc attataccga gtgttatgat acctataagt gcaagggctt gccggcagga
     4861 ccaatatgca accctggtat ggctgcaata aacgctgttc ttgagcctaa gaagactgat
     4921 tactattact tctgtaataa tcttaagacg ggcgaaacat tctatgcaaa gacacttgac
     4981 gagcatgagg aaaatcttgt taaggctgga cttgcaaaga aaaagtaata aaggattgaa
     5041 attttaatga ctgaaaataa tttgcttgaa gttcttgctc ctgtgggaga tatggaacgt
     5101 ctgtatgcgg cgctggattt tggtgctgat gcagtttatc ttggaggtac ggctttcgga
     5161 atgagagccg cctctgcaag ctttgatgct gagcttctga aaaaagcttg tgacgaggct
     5221 cacagcaggg gtaagcgtgt gtatctgacc tgcaatacgc ttccacataa tgacgagata
     5281 gtgcatttag agcagtttgt gaatgacgct cttgcggcag gcgttgacgc tatgattgcc
     5341 aacgatatcg gtgttttctc actgataaaa aaatatgcac ctgatatgga gattcatgtt
     5401 tccacacagg ctggtatagt aaattatgtt actgcaaatg aattttataa catgggtgct
     5461 aagagggtag tgcttgcaag agagctttct ctcgaggaga tcgccgaaat aagagcaaag
     5521 acccctaaag atcttgaaat tgagtgcttt gtacatggtg caatgtgcgt aagtttttca
     5581 ggcagatgtt tgctttccca gtaccttgta aaccgtgacg caaatcgtgg cgagtgcgca
     5641 cagccttgcc gctggggata ccaccttatg gaagaaaagc gcaccaatga gttttatcct
     5701 gtttttgagg acgaaaaagg aacttatatc ctcaacgcca aggatatgtg tatgctcaac
     5761 cacattgata agcttgcaga ggcaggcgtg aacagcttta agatcgaggg cagagctaaa
     5821 tcttcgtatt atgtgtccgt tatcacaaat gcctacagaa aggctatgga tatctataag
     5881 tctgatccga agcattttga gttgccacag tggcttaagg atgaagtttt caaggtaagc
     5941 cacagagcat actgtacagg atttttcttt ggtcacccaa aggagagcca gtattacgaa
     6001 aacggcgggt atattcgtga atatgatgtt gtggctgtgg ttgacggttg tagcggtgga
     6061 agaatatact gtactcaaaa aaacaaattc aacaagggcg acgagattga agtgctttca
     6121 cctgttggta gccctgtgaa gttcaccgtg gaggaaatgt ttgatgaata cgacaactcc
     6181 atagatactg caaaccatgc agctatgaag atgtcgataa gatcagatct gacattccca
     6241 gaacattcta taattcgtat gaaaaaatag caaaaaagcc atgtgatttt cctcacatgg
     6301 cttttgttta cttctttttc ttttcaattt cttcagacaa tggattcgac tccatggcct
     6361 ttttgatgtt ttcatcagat aaatgtgtgt atatctcagt ggtcgcaatg cttttgtgac
     6421 cgagtatatc tttcagtaca agagtatcta caccgccgtg ttggtacatt agggttgcgg
     6481 ctgtatgcct gagcttgtgg gtggtaatac ccaaattgct aagtcctgca cgctggagct
     6541 gttcatctac tatttgttga acacgtcgct tgtttatcct tgttttgtta gctgacaaaa
     6601 aaatggcagt ttgttccaca tggggacggg tagtgtcaat atactcatca agtgcagaaa
     6661 cacaagcgtc gttaagataa ataatgcgct cctttcggcc tttgccgagg acacgcagag
     6721 ttctgttatc tcggctgtaa tcgcttaaat tcaagcccac aagctcgctc agacgcatac
     6781 cgcagttgag gaacagagta atgatacaat aatcacgcac ctgattttta gagtcgatat
     6841 tacgcagaag ctcaaggctt tgttcaaggg acagatattt aggcaaggct tgatttatat
     6901 gtggcaattc aagatcatca agcggattgt atgaaataat cttagctttt tggcaaagat
     6961 aaacatagaa ttgtttcaaa gctgaacatt ttcttgcacg ggtttttgtg gaattattgc
     7021 gctcatcttt gagatacttt aaataaacat aagcatcgtt tagtgaaaag cattcgagcc
     7081 attctattgg cgtttctttt atttttattt tattgaaagg cgtttcggct ggaactgcat
     7141 ggtgcttgac attaagatat cgcagaaatg ttcggatatc aataaaataa gcttcttcag
     7201 tgcgatcaga acggtctttg acaactttca tgttagttag aaaatcgtca agataatatg
     7261 ggcaatcatc tacatatgct tgtttcattt atatctattc tccttattta tcacttctct
     7321 aagtttcact aaatttttat ttagtgaaac tttctgtaca cgctgtgtga ggggctgttt
     7381 gtgactcttt tacgctttgt cggctctccc cgttgtgcac tgctttaagt atacacccta
     7441 ctttgtgggc tgtcaagcta tgctacattt gcggtaatcc cttgacaaga attaagggac
//
LOCUS       DAWEMR010000127         7425 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4078_length_7425_cov_11.276526, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000127 DAWEMR010000000
VERSION     DAWEMR010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4078_length_7425_cov_11.276526"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6895..6904
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2286 a         1634 c         1774 g         1721 t
ORIGIN      
        1 gtatatgcag gacgagccgt catattacct gcccagcttc accttaacag gcttgcccac
       61 tgtcatagag tcatgggtca cgttgcagtc atagcatagt atcttcacgc cctttcggga
      121 agctttctcc aaagcctctc caaactcagg gtgagtgtca taatttggag tgaaatattt
      181 cacatcgctc atctgaacga gtatcattat ataagactca tacccctcat cgaggcagtg
      241 agaaagctcc tctatatgct tcaccccacg gagggtagga gcgtcaggga aaagcacaac
      301 gccgtctttc tccagcgtac agccctttac ctccatgaag atcttcctat cctgcgactc
      361 taagtaaaaa tcaaagcgtg aattgccgta tttcttttcg ggatagacag agaagccctc
      421 gccaaaaggc attttttctc tcagccactc ccctaccgcc ttattcgggg cttggctgtc
      481 catgtttatg agcctgtcac ctttttccac tgcaacaagg tcaaaagcgg tctttctcga
      541 ttcgctgtct gaatgttcaa gccaaacagt acacccctcc accaaaagtt ctttgcatct
      601 gcccgtattt ttcacatgac agacctcggc tttaccgtct atctcaacgt tggcgataaa
      661 cctattcggt cttgatagaa atttgccttt atgtatatta tcgtatctca taattcgctc
      721 cttttattct tactattcac attataccac aaatatgttt gattataaat acctaaatca
      781 tgcattctta gctttgctga tttgtggttc gatacgaaaa gggaaaaata tttcacaaaa
      841 tctattgacg ctatttgtga aatatgttat agttttgagt ataggttata taacaataat
      901 aatttattaa aatttcagaa agaggtattt ttatgaaaaa aataactgca cttctcgttt
      961 cacttataat gtcatgcgca gtaatggcag gctgttctga cagcagttct tcaagctcat
     1021 caaaagcaga cagcacatca aaggcagaaa caagcgcagc cgctgacgag tcaactgctt
     1081 cagaagcagg cacagccaca gatctcgagg atactctcac agcgcagtat acaaagaaga
     1141 tacagggcga gagccttgct atcgacatga cagtggcaag cgaatatgac aattcatcag
     1201 ctgttgttga gatagcaggc ggaaatatgc acatgaaaat gaactttatg ggcgtggata
     1261 tggacatcta taatatcgac ggagttatgt acacacttga cagcgagtca aaaacgtatt
     1321 acactgttga tatggaagaa tctatcgacg acttcaagag cgaaataggc tacggcatta
     1381 acgacagcta caagttcata agctctgaaa caactgacga cggtcttatc tgcgaaactt
     1441 ttgaaaacac agaagatcct ggcatcgagc ttgaatcagg cgtaactctc gacacaagcg
     1501 attctgacga ttattctttc acaacaacat tcaagtatta cttcaatgct gacacaaagg
     1561 atcttgttaa gatagaaact atctctgacg gctcaacgac tactgttaca gtaaactcat
     1621 tcacaactga aaacgttgaa gtcaagctcc ctgacgattt tgacagttgg acagagcagt
     1681 ctttcgataa ggacactgaa gatatccctt atgacgctga actcgatgtt gagtcaggcg
     1741 ctgagtcagc cgagtgattc ttgtcatttt tcggaggcaa aatattatga acaagaaaat
     1801 ggtaactatt attgcagctt tggcaatgtg tacagcaatg gcaggctgca ctgggaacga
     1861 tactgcaagc tcttcaaagg ctgaaacaac tactacaaca acggctgccg cagagtctgc
     1921 cgctcctgct gaaagcacag cagacgaaag caacgctgac agcacttccg caaacgtagg
     1981 cacaacggat tatgacgttg acggtgattt cagagctttc gagcagatga aagacaagta
     2041 tgagggcaac tatcttgcga accttacagg tcagatgtca aacaacggca caaagtatac
     2101 tatctgcata aaggacggca aggcttatca ggcactcaca gttcagggca tgaagtcata
     2161 cacagtttgc cctggcgacg gaaagcttta caacgtttca gaaggcacga ccacctacag
     2221 cattgaggac gacgacggaa atcagtggaa gtcagccgac attcttttcg gcgcgaccct
     2281 cgatttcgac cacgcatata ttgacacaac aacaaacgtt gttcacgagt attataaact
     2341 cgacagcagt gtttcaggcg gcgagggtca gatagtttac ggctttgacg gtgctacata
     2401 tgaccttaag caggtagttc tgctcccaga gggtgctgac gaatcaagcg cagtttactt
     2461 cactgtagat tccataggcg aagcggacga cagcctgctt gctgttcctg atctttctgc
     2521 ttatacaaaa gaataatttt atctgatctt tttccaacat cgggcggaca ggccactgcg
     2581 gtctgcccgc tttttttgca tttccagcaa agcgttgtgc aatatgacgg aaaagaagtt
     2641 caaatatttt tgaaatttgt gagaaaaata gtcttaaacc ccttgacaaa atcgtcaaaa
     2701 gggtataaac tatacttagc actcgagaag aaagagtgct aacacttaaa tacaataagt
     2761 gctaacaaga agaacgggtg gtgagcaaat ggacgaccgc aagctgaaaa tactttcagc
     2821 ggtagtcaat gaatatatcg taacaggcga gcctgtaggc tcaaaggcga taatggctca
     2881 cgtcaaagcg tcctcagcga ccataagaaa tgaaatggca gagcttgaga agcaaggcta
     2941 tctcgaacag ccacacacct ccgcaggcag aataccaaca tataaaggct acaggcttta
     3001 tgttgaccag ctcatggagc aaaaccagct tacagcaaat gaaaagaaga tgctggattc
     3061 aatgataccg caggaatatg tcacagagga agaccttgtg aacaaggctt caatggcatt
     3121 ggcggatctt acgaaatgtg cggcggtggt cgcaaacgca acgccaaagt tctcgcttat
     3181 ctcaaaggta gaggtaatac ctacaggaaa gagaatgtac gttattttga tgataacgtc
     3241 caacggctct attaagaata aggtttgccg attggagttc gacctcagtc aggatcagct
     3301 tgagtttttt gataacttcg taaaggaaaa tctcaacggc gtgcctatcg acagtctttc
     3361 ggacgcttat cttgaaaagc tcacagaagc aatgggaacg tacatgatga ccctttcacc
     3421 gcttgtttcc gagataatga agatgacaaa ggaaatgagc agcgtgtcta tcaaaggtca
     3481 gagtaatctt ctcacctgca aggacttcaa ccaaaatgag ataataagct ttctcgataa
     3541 tcagaaggag cttaaaggat ttattgacga aacgttctca gggcttcacg ttatgttctc
     3601 ccctgagcgt gacgggtttg ttatccacaa ctcctctatt ataacagctc cgttctcaaa
     3661 ggacggcaaa acagcaggtg cgctggggct tataggtcct atgaggctcg actatgcgaa
     3721 gtttatccca tatctcgagt atttcacagg aaagatatcg gaaatgatat ccgaaaagcc
     3781 acaagagcaa aacaaagaag atgacgaaaa tgaataaaga gaaaggcagg cggcgtaaat
     3841 tgagtaagga aactgaaaag gacaccgagc ttgaaaaaga ggaaaacacc tctgctgaaa
     3901 gcgaagaagt gaaagaagcg gcggataccg cagaaaatgc cactgaggat acaaagaaaa
     3961 aggcggagga gagcgaaaca gaaaagctga aaaaagagct tgccgaagca aatgacaagt
     4021 atctcagact tatggctgaa tatgataatt tcagaaaaag aagcgcaaag gaaagactgg
     4081 agcttacaga cacagcaaag ggaaatgtga taaacggctt cctgccggtg tttgataact
     4141 ttgaaagagc gttaggcaca gaaactcagg acgcagttta taaacagggt gttgaaatga
     4201 tcttcaatca gttcagcgac gctctgaaaa aactcaacgt tgaggtaatg gagcttgcgg
     4261 gcaaggaatt tgacccgaac gttgcaacgg ctgtaagtac agttgacgac cctgacctcg
     4321 gtgaaaatat tgtggcgcag gtgcttcaaa acggctacac cataggcggc aaggtaataa
     4381 gacacgcaat ggtaatagta gcaaacccat aaacaaagac atcgggactt aaggtcttga
     4441 tataaattaa gtaaattttg aaaggacttg atcttatgag caaaatcatc ggtatagatt
     4501 taggtacaac aaactcttgt gtagcagtta tggagggcgg cgaagccgtt gttatcccta
     4561 acagcgaggg tgcaagaaca actccgtcag tagttggcgt ttcaaagaac ggcgacagac
     4621 ttatcggtca ggtagcaaaa agacaggctg ttacaaacta tgacaacact gtaatttcta
     4681 tcaagagaca tatgggctct gactacaagg ctcacatggg cggcaaggat tacactccac
     4741 aggagatctc agctatgata ctccagaagc tgaaagctga cgctgaggct tatctgggtg
     4801 aaaaggtaac agaggctgtt atcacagttc ctgcatactt cacagacgca caaagacagg
     4861 ctactaagga cgcaggtcag atcgcaggtc tgacagttaa gagaatcatc aatgagccaa
     4921 cagccgctgc actttcttac ggtatcgata aggaagagga tcagaaggtc atggtttacg
     4981 acctgggcgg cggtacattc gatgtatcta tcatcgaaat gggcgacggt gttacagaag
     5041 ttcttgctac agcaggtaac aaccacctgg gtggcgacga cttcgaccag agaattatcg
     5101 actggatgat cgcagagttc aagaagactg agggtgttga cctctcaggg gataaaatgg
     5161 caatgcagag actcaaggag gctgctgaaa aggctaagat agagctttct tctactgctt
     5221 catcaactat ctctctccca ttcatcacag cagacgctac aggtccaaag catcttgaaa
     5281 tgacacttac acaggctaag ttcaacgaaa tgacagcaga ccttgttgag tcaacaatgg
     5341 gaccagttcg tcaggcactt tcagactcag gtctgtcagc taacgagctt cagaaagtcc
     5401 ttatggtcgg cggttcttca agaataccag ccgttcagga agctgttaag aaattccttg
     5461 gcaaagagcc attcaagggc atcaacccag atgagtgcgt agcacttggc gctgcatatc
     5521 agggcggcgt tctcgcaggc gacgttaagg acggtctgct cctcctcgat gtaactccac
     5581 tgtcacttgg tcttgaaaca atgggcggta tctgcacaaa gatcatcgag agaaacacaa
     5641 ctatacctac aaagaagtca cagatattct caactgccgc tgataatcag tcagccgttg
     5701 atatcaacgt acttcagggt gagcgtgagt tcgcaaagga caacaaacag ctcggtatgt
     5761 tcagacttga cggaatcgct cctgctccaa gaggcgttcc acagatcgag gtaacattcg
     5821 atatcgacgc taacggtatc gtacacgttt ccgctaagga tcttggcaca ggcaaggagc
     5881 agaacatcac gatcacttct tcaacaaata tgtctaagga cgatatagat aaggctgtca
     5941 aggaagctga ggcatatgca gcagaggacg ctaagaagaa agaggacgtt gacgcaagaa
     6001 acgctgctga ccagctttgc ttccaggctg aaaagtcact gggcgagttc ggcgacaagg
     6061 tagacgctgc tgacaagtca gcttgtcagg ctaagataga cgctcttaag gaagccctca
     6121 agggtactga tatcgaggct atcaaggcta agcacaagga gcttgaggaa acattccagg
     6181 gcgttgcaac taaggtatat cagcaggctg cacaggcaca gcaggcagca ggcggtgccg
     6241 caggctttga ccctaacaac atgggcggag caaatggcgg cagcaacggc ggcgcttcaa
     6301 acaacggtgg cgacgacgtt atagacgctg aatttactga cgcagaataa atcataacgg
     6361 ctgcctctgc tccgattttc cgagcagggg ctattgccgt attaaaaggc tgtgtgaaaa
     6421 gcgtgaaagg actatactta caaaaaggag gcaagcccat gccccaagac tttcacgatc
     6481 tgcagcttac agagcatggg cgtttatatg gctgaaaaaa gagattacta tgaagtgctt
     6541 ggcgtttcta aaactgccac agacgatgaa ttaaaaaagg cattccgtaa gcttgcaaag
     6601 cagtatcacc ctgaccttca ccccggcgac aaagcgtgcg aggaaaagtt caaggaagta
     6661 aatgaagctt atgaggtgct ttcagataaa gataaaagag caagatatga ccagtttggt
     6721 cacgcaggcg tagacccgaa ctacggtgca ggcggtggag caggtgcagg aggcttcggc
     6781 ggatttggcg atatgggcga tattttcgat tccattttcg gtggatttgg cggaggcttt
     6841 ggtggcggac gttctgctaa ccctaacgca ccaagacgtg gcgaggatat aagannnnnn
     6901 nnnngcgaac gttgttcttg acttcatgga agcctgcaag ggcaagactg tgaaacttcg
     6961 cataaacaga gccgaaaaat gtcctgactg tcacggcaca ggtgcagccg caggttcttc
     7021 gcctaagacc tgtcctgact gccacggcac tggtacagta aggataacac agagaacgcc
     7081 tttcggaaac atcgctcaga ccacgacttg cagtcgctgt ggcggtaagg gtcagataat
     7141 cgacaaccct tgccatacct gcaacggtca aggcagagta aagaaggtat ctgaaaagga
     7201 gataaacgtt cctgctggca tcgacgatgg tcagacactt agagtaggcg gtgaaggcaa
     7261 ctgcggtata aacggaggtc ctaacggcga cctgcacatc aacattacag ttcgacctga
     7321 ccctattttc gagcgtgacg gctatgacgt gtggacagag ataccgctta cttatgcaca
     7381 ggctactctc ggtgacgaga taacagttcc tacagttgac ggcaa
//
LOCUS       DAWEMR010000128         7372 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4114_length_7372_cov_8.963100, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000128 DAWEMR010000000
VERSION     DAWEMR010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4114_length_7372_cov_8.963100"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1731..1740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2477..2486
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1951 a         1458 c         1822 g         2121 t
ORIGIN      
        1 cccaaataag agcctatttc caacattgcg gtgtatttgt cgatacacac gataacgtaa
       61 gattcagcgt atttagggaa tttcagggtc agcggccaca tatgcttgac tatcatatcc
      121 tcctcacgct tgcctatctc aaaatgctgt ttcgcattgt tgagtgcgac ctttgggtgg
      181 gtcattccgt gaggcatttc gccttttttc ttctcacgat cccgccaatc ataaagaaac
      241 agatcgtgaa gcagacctgc tcttgctgcg gatcttgaat ttagtcccaa ttttctgcaa
      301 acgagatagt tatagtagga tacattgatg caatgctgat agcaagtcgt actatagtga
      361 tgacgaaaat ttttcatttc gtttacaaca tcgctgtcaa gcaggtcctt tacaaactca
      421 aaatactcct ttgcttcctc ggaattttca atggaatatc ttgaaccgaa agcctttgac
      481 atttgcatga cctcttttcg ataacttata tgcaggcggc aattttgctg ccctttttac
      541 ggttggtgac taatgtttac cccataagtt accccattca agtttgtttt accaaactta
      601 cattaacatt ataaactttt ttgttcaaaa tgtcaatagg gaatgtataa caattttatt
      661 acaaaaatat cgggctgtct gtgttagcat ggcagtccga taaaaaattg gatatcagta
      721 gtatttttcg tcataaaatt ttgggataaa atctttgacg gcggatgtgc ggctttggac
      781 aaaaccctca aaaccgtatt tattcacggc gtcaatgacg gtgttatact cgaaagttga
      841 tgtcttacgg caaagctctt tgtagtcaaa ggctttatac acgggggtga actggctcat
      901 aagacttatc attatttcgt ttttggcaaa atttttgcct agccactcca taagctttat
      961 ggagtcttta cggtgggtag ggaggacaag gtggcggact attgtgccgc tctgcattat
     1021 gcccttttcg tcaaagattg gcttgcctga aagctccgcc attttatgta ttgaagcagc
     1081 ggcgttttca aaatagtctg cggctttgga atatttatcc gaaagtgtag gatcaaaata
     1141 tttaaggtcg ggtagaaata tctgcacata gccacgaaga agctccagcg tttggggctt
     1201 ttcataacca ccgctgttat agaccacagg gatagtgagc ttatctttac agaggtcgag
     1261 tgctttgatt atttgcggaa cgaaatgggt gggattgacg aggtttatgt tgtttgcgcc
     1321 cattgattga agccgcagaa aagtgtctgc aagttcttgt tcggtaagct caaagccttt
     1381 gccctcagcg gatatctcgt agttttggca gaaacagcat ttcagacaac agcctgagaa
     1441 aaatactgtg cctgagcctt ctgtgccact gatacaaggt tcctcccaca tatgaagctc
     1501 ggctcgggct atccttactt tggcagtttc accgcagaag cctttatgct cgtatcggtt
     1561 cactttgcac tctcttgggc agagggtaca gcttttaagg atatcgttca tgccgctgtg
     1621 tcaactccgc ctattttggt gtaggagtca gaggataggt cgcctgatga gtctgtgttt
     1681 ttgtcaaggg ggatattgaa ctggaatgaa tcggttatcc aatcgtcatt nnnnnnnnnn
     1741 tatcggcagg gacaagaaac ttgatcgtat aggtcatatc gtctccgtag tcgctttcac
     1801 cggggaagaa gttgtttgag aaaaacgaat tatcgttttc gtcataggcg gtgttgtagg
     1861 caaaggtgaa ttttacctgt ctgttgctat cactgatatt aacatcaagg tcgaggtcta
     1921 tttcccatac tgtgtcaccg cttataatac cgcctgaaag ggagtttctt tcctcatcgg
     1981 tcaggtcgct tactttgcgt tcggtgaggg tatagccttg atactgaacg ctgtaggtac
     2041 cgtcctcagc gtcttgcgct acatagaggt tggaatggtc ggtgtagtca tcttcggctt
     2101 cactataggt atcgtcataa tcgtagtcat agttatagtc gtcatatttg cttgaaatta
     2161 tttttgatga gacaaagcct atgatagttg ggaggatagc cataaggatt attatgacca
     2221 caacggcggt ggcacagcct ttgccgccat tctttttgtt agagtggttt atgttagtgg
     2281 cgttgaagct ataggagctg ttgtttgggc ggttatagct tgtacgctgt tggctgtagt
     2341 tattgagggc ggcaagcttt tcggctcttg aatttgaata gcttggggga ggggtcgggt
     2401 ttttaggtgg ggtataggtg ctttggaaat tctcgtccct tgcttcctct ttacgttcct
     2461 cggaaacgaa atcgttnnnn nnnnnngccg tcctgctcgt tatcggcacg gagatcatga
     2521 gagtgcaggt catctgaaag gctgttacag cttatatcct cgtcacgggc tgtgaagcac
     2581 tcaggacaaa ttatctcatc atcgctttta aagtggtatc cgcaaattct gcatttattt
     2641 ctgctcaatt atttcacctc attaatatac aaattcccag ccctgtttta caggctgaca
     2701 aacacactta tagctccagc ctgacagctt ttctgcggct gtttgtgcgg aagcgatatc
     2761 ggggaaaact ccaaaaacgg ctgagccgct tcctgtcata agggcggaga gagctccgct
     2821 gtttttaagc tcatgcactg catgagatat ctcctcacgg tcggcggcat attccagcac
     2881 attgaaaagg tttgagcata tgccgaaaag ctttccacca ccgatatatc tcagcaggac
     2941 gtccatatta cagcgtttgg ggtttgaaat gctgtcatac tttttgaaag cttcgggggt
     3001 ggagataccc acgtcgggtt ttattattac gaaagcgcag tcgggtgagg gcaggttgct
     3061 cattatttcg ccaacgccct gacagagcct tgtgccgcct gtaatacaga aaggcacgtc
     3121 tgcacccaga gccacgccta tggagcagag ctgttcatcg gtgagccttg tatcgaacat
     3181 gaagtccaag gctctaaggg tagcggcggc gtctgcactt cctccgccca tgccggcttg
     3241 tgaggggagg tttttctcca cattcactgt tattttacag ccgaagtcaa tatgggtgaa
     3301 gtcggcaaag gcatggtagg cttttgtgat gatattatcc tcgccctttg gaaagctgtc
     3361 aagtttgcag gtaagctcta tgccctcgcc ctcagagagg gtgaagtcca ggaggtcgaa
     3421 gcaggacact gactgcatga cagtatttag catatggtag ccgtcatcac gtttaccaac
     3481 gatatccaat gcaagattta tttttccgta ggcttttatt tttaaattac tgcacgttac
     3541 catttttgat atcctctaaa aattctccga tacgtttgat agcctctctc aggtggttaa
     3601 gggaataggc gtaggagaca cgcacatagc cctctccgca ctcgccgaaa gcatctcccg
     3661 gcacgacggc tacttttttg gtgtacacaa gcttttcgca gaagtcggtg cttgtcatgc
     3721 ctgtgctttt gatacaaggg aaaacataga atgcgccttg tggtgcaaag catttaagac
     3781 caagcttatt gaaagaatcc accacaaagc gacggcggat attatactcg ttacgcatgg
     3841 catctatatc cttctgacaa ctgtgcatgg caacgatagc ggcatactgg ctgacagtag
     3901 gggaggacat gatagcatac tgatgaagct tgagcatttg ggacactatc tcttttggtc
     3961 cgccaaggaa gccaagccgc cagcctgtca ttgcaaagct tttggaaaag ccgtttatga
     4021 cgatagtacg ctcacgcata ccctctattt ctgcgataga gcaatgcttg cctgaatatg
     4081 taagctcgga atatatctcg tctgaggcta tcatgatatt ggtatcttta agcacctttg
     4141 cgatagcttc aagatcttca cggcgcatta tcgcacctgt tgggttatta ggatagggca
     4201 ggacaaggag cttggttttg tcggttattt tttcacgaag ctcgtctgct gtaagtcgga
     4261 actcgttttc ttcctttgtt tctatgacca cgggaacgcc gccgcatagg gagacgatag
     4321 gacgataaca gacaaaggac ggttcgacta taaggacttc atcaccgggt tggataaggc
     4381 atcttatggt aaggtcgata gcttcagagc cgccaacggt gacgattatt tccttattag
     4441 ggtcatagct tgtgtggata gtacgctgaa aatacttggc tatctcttca cggagagcga
     4501 tcagacctgc gtttgcggta tagcaggtct tacctttttc caaagttgag atagcttcct
     4561 gacgtatgag ccacggagtt ttgaagtcag gctcgccaac gccaagggag ataacgcctt
     4621 caagctgctg tgcgatatcg aaaaattttc tgatacctga gggcttcatt tcctttatgg
     4681 cagggcaaag cactttatta taatctatca tggggagatc aaacctcttt cgtctttatc
     4741 atctgtgctt atcatgatgc cgttgtcttt atagaccttt agcacgaagt gtgtggcggt
     4801 ggactggaca gcgtcgatag ttgcaagtct ttgggaaaca aactgtgaga tatccctgat
     4861 atttgcaccg ttcgcggcaa cgattatatc ataagcacct gacataaggc ggacggactc
     4921 cacctgctca tagcttgcga tctggtttgc tatctcgtca aagcctgact gtgactgtgg
     4981 ggtgacttta agttctataa gagcgaaaac tgaattaggg tcataggcac tttcgtcaac
     5041 gatcgcggaa tagcccttta ttacgccgtc tgcttcaagc ttggcgatat ccgctgcaac
     5101 ctgctcttct gttttatcaa gaagaacggc aagctcgctg tttgtgatac gggcgttgtt
     5161 tttcagtaat tttataagct gatccataaa taagcctcct ttaggtttat aaatatacat
     5221 tatatattat aacatattat ctgtatgaac gcaagtattg tgatgtaaga aatgagtaat
     5281 ttatctgtca gaatagacaa aggggagggg gagaaagctt gtggcgtggg ggtcgtggta
     5341 tagccgagat gttgtggggg tgaacgggcg aacgctgttc gcccctacag ataaattgcg
     5401 gtcatgagaa attgtagggg ctggagtcct cgacagccca tttatcttgg gtatttattt
     5461 tatgagctgt acagttattt ggactgtaca gctttttatg tgtaaggggt gtgaaaattg
     5521 gggcggcggt attgaaatta ttattttgtt gtgatataat tgtactaact agaactgtgt
     5581 aaattcagga aggatcttta tgagtataaa cagcaatgag ggcaatgatt tttgcaacag
     5641 atataatgag gtgctgaaaa gcgataagaa aaaaatgatc gctgcggctg cagcttgcac
     5701 tatcgttatg ggagcaggct ttgggggata tttatactac ggcgcttatt atgcttcggg
     5761 ctggcacacc catgacggct cgacttatta tattgtcaag gagacgggcg aaaaggcgct
     5821 tggctatcag ataatagaca acacctgcta tctttttgac gacaagggaa acgctatggc
     5881 tgacggctgg cataagtatc gtggggacac ttactatgtc aaggacggcg ttgttcagcg
     5941 tggaaaaatg aaaataaagg gagaggaata ctatttctct gaggagtctg gcattttcag
     6001 gacgggcctt tgcgagataa acggcggtga atactacttt gacgatcacg gcttccctga
     6061 cacgggtttt gacagcgacg gcggttatta ctatgacgag agtggcaagc gtgtgacagg
     6121 ctgggcaaag ataaacaatg ttcagtatta ttttctcaag agcggtgaga tggctaaggg
     6181 ctttgttgag atagagggca agatatacta ttttgatgat gacgacggtc acatggccac
     6241 aggctggcag gacattgacg gaaaaaagta ttatttttct gagagcggtg ctgttcacaa
     6301 gggctggatg gagcttgaaa agaagtatta ttattctgat gaagctacag gggcttgcgc
     6361 tcagggcttt gcggagatag acggtgagag ctattatttc aatgatagct gtgaaatggt
     6421 caagggctgg ataacaattg acaagaacag atatcatttt gctgatgacg gtaaaatgac
     6481 taagggctgg tatgaagaac cgcctgagaa gtattatttc aagggcgacg gctctgcagg
     6541 caagggcttt accaagatca aggacaagta ttattacttt gataagaaaa atagactcct
     6601 ctccggctgg aacgagatag gcggaaatgt ttattacttc ggcagaggcg gcgttgtggc
     6661 tgacggctgg gaggatattg acgaagatac ttactatttt gacaaaacga cccatgtggc
     6721 ggcaacaggt tggacgaata cggatcagta cactgatgat gaaaaaaaga aaataaaaga
     6781 gttcaaatcc aatgtatcaa agcttgtgaa gtttgagaag gacgattaca aaaaggacga
     6841 gaagcctgac gaaaaagaga cccaaaagct tgaggagctt gcggacaaat tcggtgaaaa
     6901 gacattcaat gcttatgaca gaaaggttta tgaaaagttc ggcggggcgg tgttctatca
     6961 gtattatttc tactctgatc acactctttg cactggattt cataaaataa acggttatta
     7021 cttctacttt gatgaggaaa ccggcaagaa ggctacgggc tggaagacca ttgacggaaa
     7081 gagatattat tttgatccgg aaacaggaat catgtacaaa aactgtacta ttgagaaaaa
     7141 cggaaagagc tatacattcg atgaagatgg tgtatacaca actgtatccg catcaaataa
     7201 gaaaaaataa aaaaatgccg gcagcgaaca taaagctgcc ggcattttta tgatcataag
     7261 gtataaacaa ggattcccag gatgatgatc agatttccga tgatctttcc gcgggaaatc
     7321 ttttctttca ggatccgcca ggaaaacagc aggacaaaaa cataggcggc ag
//
LOCUS       DAWEMR010000129         7364 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4126_length_7364_cov_9.588453, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000129 DAWEMR010000000
VERSION     DAWEMR010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4126_length_7364_cov_9.588453"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5121..5130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2241 a         1405 c         1844 g         1864 t
ORIGIN      
        1 gtataagggc gacaagcgga cggagttcag ctttgttgca aagaatgata tcccacccct
       61 tgacgtgaac gagcttccaa aactcgatga tgttttcagg atacttgcac catgctttcc
      121 tgcaataaga aattcctatg agctgtggct tacggatact tcccacagag taaggagagg
      181 actttcacag tcattcctgc ttggaaacta caccactgcc actatacagt atatcataga
      241 tggagtggca cttgtgggac acgttggaac gatacctgag gagcgtggaa agttccatgc
      301 aagacagctt ttgtattgga taggtgaaaa gctcacgcag gacggctttc aggttagatt
      361 gtttgcaaga ccccatagag tgagctatta tgaggagata ggctttaaag cctgcggaat
      421 agatcttgta ttggagagaa ttgaaaatga atgaatgtat atttgatata gacccaaagc
      481 ttttatctct tgcctccgag gcagagaatg agtgcaggga gatgtttgag aaaatagaca
      541 gcaacgccga gtataacgga cagaaagtgc tgaaagcttt tatcgacaat cgtgtgagcg
      601 agggctgtct taaaggaaca acgggctacg gatatggcga catgggcaga gatactatag
      661 acaaggtgtt cgctcaggca cttggtggcg aagatgcttt ggtaagacac acctttgtga
      721 acggcactca tgcactgtca acagcgcttt tcggagtgct tagaagcgga gacacgatgc
      781 ttgcctgcac tggaaagcca tatgatactc ttgaggagat aatcggtatc agaggtgaaa
      841 agggcaacgg ctcacttatt gatttcggtg taaagtatga gcaggtagac ttggtggacg
      901 agatgtaccc tgattatgac gagatagcaa agaaagccgt tggcgcaaag atagcctata
      961 tccagcgttc aagaggctat tctcttagac cgtcactgac agtggagata atcggaaaga
     1021 tagcagaaac agcaaagaaa gctaatcctg atataatcgt catggtggat aactgctacg
     1081 gtgagtttgt tgaaagaaaa gaacctctga gcgtgggtgc agatatcata ataggctcgc
     1141 ttataaaaaa tccgggcggc ggaatagcct ccacaggcgg atatatctgc ggcagagcag
     1201 accttgtgga aaagtgtgcc gacaggctca cctgcgttgg catgggcaag gaagttggtt
     1261 gttcacttaa ccagaacaga gaaatgctcc ttggcttttt cttagcaccg caggtggtcg
     1321 catcagcact gaaaacttcc gttttcgcct gccgtttctt tgaaaaactt ggctacaaga
     1381 cccttcctga gagcggagaa acaagaacgg atataatcgc atctatactt cttgaaaacg
     1441 aggaaaacct cgtggctttc tgtcagggaa tacagaaagg ctcgccagtt gacagctatg
     1501 ttactccaga ggcatgggat atgccgggtt atgacagcaa ggttataatg gcggcaggag
     1561 cattcacaat gggtgcgtcc atagagcttt cagcagacgc tcctataaga ccgccgtttg
     1621 ccgcatggct tcagggcggt atcacatacc caacaggcaa agcaggcgtt atgcttgccg
     1681 cacaggagat gtataacaaa ggtttactta gaatataaga tttgactaga aaggaatgat
     1741 attcaatggc agagatattc agcgttactg tcgaggagat aatcaaacag ctcaatatgg
     1801 agataatcta tgctcctgag aacatcagca gccttgttgt tactgagaat gattgtaaca
     1861 gacctggact tcagcttatg ggattttatg agtatttcaa tgcagagcgt gttcagatat
     1921 gcggaaatat ggagtttgct tatcttgctt cacttgacga gaaaacaaga tatgagagga
     1981 tagacgctct ttttgctaca aagataccgc tttttatcgt ggcaagaggt catgaacttt
     2041 atcctgaaat ggttgagata gctaagaaat acggcgtacc tatcgcaaga actcaggaca
     2101 gcacaacagc atttatcgcc gcacttatcg gctatttgaa cgttgaactt gctccaagaa
     2161 taacacgcca cggtgttctt atcgaagtat acggcgaggg aatccttatc gtgggcgaga
     2221 gtggtgttgg taagagcgag acagctatcg agcttgtaaa gagaggtcat agacttgtgg
     2281 ctgacgatgc cgttgagatc agaaagacat caaacagaac gctagtaggc tcatcacctg
     2341 ataatatccg tcacttcctt gaactgagag gtatcggtat catcaacaca agacgtctgt
     2401 tcggtatggg tgctgtaaag gtaagcgaga agatagacct tatcgtagag cttgagcctt
     2461 gggacagcac aaagatatac gacagaatgg gcgtagataa cgagtatacc actatcctcg
     2521 gtataaaaat acctagcctt accattccta taaagccggg cagaaacctt gctgttatcc
     2581 ttgaagttgc ggctatgaac aaccgtcaga agaaaatggg ttataacgct gccgctgaac
     2641 ttttgcagaa tcttggtctg caaatggaca agaaagacaa ggtcaagaac tgggataatt
     2701 tctaaaaaca cgcatatact tttttttaat aggagataga tcataatgca cttacaggga
     2761 gatatgcaca cgcacactat agcttcgact catgcttact ccaccataac agagaactgc
     2821 cagtgggcgg caaaatacgg aatgaaaggc atagcaatga cagaccatgc catgaatatg
     2881 cctgattcgc cacatatctg gcactttgtg aaccttggca tactgccgag aaagataaac
     2941 gggataaccg tcctcagagg catagaggct aacgttgtga gcgatgacgg agagcttgat
     3001 accagcgaaa agctgctttc tggacttgaa tgggtggtgg cgtctatgca cagtgcctgc
     3061 tatactccct ctacacctga gaaccacact aaaagctggc tgaaagtcgc acagaaccct
     3121 caggtggatg ttataggtca ctgcaccacc gcacctttta tgttcgatta tgaaaagggc
     3181 gtaaaggcgt tcaaggagta cggcaagctt gtggagataa acgaaagctc atttgcgtgc
     3241 aaaaggtcgc ccagaagaaa cgtagtggag cttttgaagc tttgcaaaaa gtatgaagtg
     3301 cctgtggtct ttgactctga ttgtcatttc tgtcagctta tcggtcaaac accgaaagtg
     3361 gcaaatatag cggaggaact tgattttcct gagaagctta tcatgaacag tgattgggaa
     3421 aagataaggg aatatgttct tgcgaaaaga ccaaacctcg atatttaaaa cgatgaaagg
     3481 agaagccctc acaagggcgt tttacagata tatatgaata cacaaaagct gcttgatctt
     3541 gcctcagagc ttgaatgtaa tgttgtctac aacgagccgc tttcaaagca taccacattc
     3601 cgcaccggtg gagcgtgtac tgcaatggtg gatataagct cagaggaaag ccttgcaaaa
     3661 cttgttagag ccgcacacga atggaacgta agatatattg ttgttggaaa tggctcaaat
     3721 cttttatttg acgataaggg ctacgaggga gtggtgttcc tcatgggtca gtcagtggac
     3781 gagataaaaa tgatgaacga aaacaccata tacgccgagg ctgggtgcag ccttataaaa
     3841 ctgtgcagat tcgctcttga gcataacctc acaggacttg agtttgccta tggcataccg
     3901 ggaactgtgg gcggtgctat atatatgaac gcaggtgctt atgacgggga gattaaaaac
     3961 gttataacct ctgcgaactc cgtgagggca gacggtactg tacatactac agaggcagat
     4021 aaaatggctc ttgcttatcg cagcagttgc tatcagaaaa acggcgaagt cataacctca
     4081 ggagtgttca gacttgaggc aggagcgtat gacgatatac aggacaaaat ggtggagctt
     4141 atgggcagac gccgttcaaa acagccactt gaatatccaa gtgcaggctc gacttttaaa
     4201 agacctgagg gacagtttgc aggaaagctc attgaggact gtggtctgag gggatacaca
     4261 gtgggaggag cacaggtgtc tgaaaagcac tgcggttttg tggtgaacaa gggtggtgca
     4321 acaactgagg atattatgtc ggtgataaga cacgttcagc acattgtcaa agagcagaca
     4381 ggctatcttc ttgaatgtga ggtaaagata ataccttaca aggaataatt taaataggga
     4441 tcaataagtg atgatttttt tcagctttgt aagaaaggaa tgagcgttat gcagtttgtg
     4501 ataattactg gtatgtcagg atcgggaaaa tcaagtgccg tgaatgtgct tgaagatata
     4561 ggatattact gcgttgataa tatgccgcct gagcttatcc caaagtttgc aaacatatgc
     4621 agtcagtctg acggaaaaat agacaaggta gcgtttgttg ttgatataag aggcggcgac
     4681 ctttttctga agcttcaaga cacagtaaag cagcttcagg gcgagggcgt gagtcttaaa
     4741 atacttttcc ttgacagctc agatgacgtt atcgtgagaa gatacaagga aacaagaaga
     4801 aagcaccctc ttgacgaggt gtcatatgga aacataagaa aagctattga aactgagcgt
     4861 gagatattaa gacctataaa agcacaggct gatttttata tagacacgtc cagaagctca
     4921 acagctcaat ttaaggaaag cctttattcc atattccttg gaaacaacga gtttatgcat
     4981 attgacgttc agtcgttcgg gttcaagttc ggtgcaatga gcgagggcga ccttgtattt
     5041 gacgtaaggt gtctgcctaa tccgttttat gtacctgagc ttaagaacaa aacaggtctt
     5101 gacaaggacg tttatgatta nnnnnnnnnn gatatctttt ctcactgaaa acaaagcggt
     5161 agcagacttt ttcgtgttga acaccggcag gataactggt aatgaaaacg cagtgttagt
     5221 cacagagcag aaaaagcgaa acggcgttgt gctttatgct ttggatattc ctgagcgtga
     5281 ggacaggctc acacttcttg actatttcag aatggataag agcaggcgtt tggaagaagc
     5341 tgacctgcct aaagagaagt tttatccaca gctcgtggct ggtatatttc ttgcctgcgg
     5401 ttcggtgaac aatcctgaga aaaaatatca tatggagttc gttatgccaa cgatagagct
     5461 ttgcaatgat tttggtatgc tgcttataga taattacgat atcactccaa agcacgttga
     5521 acgtaagaac gcacagatag tttacataaa agagtctgag aatatcatag atatgcttac
     5581 tcttatgggt gcgcctatgt gttcgcttga acttatgaat gtaaaaatga tgaaagatat
     5641 gcgcaacaag ataaaccgag cggtgaactg cgataacgcc aatatagaga agtcgctgaa
     5701 agcagcggaa aagcagataa gcgatatcga gcttatagac gagacagtgg ggctttattc
     5761 tcttcctgat aatttgcagg agatagctga gcttagatat aataatcccg actacaatct
     5821 taaagatctt ggtgcagcca tagatcctcc tatctcacgt tctggggcaa accacagatt
     5881 gcagaagata gcagagatag ctgacaggat aagagttgaa aaaggtcttg cactgaaaga
     5941 ggaataaaaa tgcctgagtt tgtacatcta catcttcata gtgaatattc gctccttgac
     6001 ggcgcctgcc gtatcaagga gcttgttttg cgtgtaaagg agcttggaca gaaagcagtt
     6061 gcggtcacag accatggaaa tatgtttgcg gcagtggagt tttacaacga atgtcttgct
     6121 cagggcataa agcctataat aggctgtgag gtgtatgttg caccaagaac acgatttgac
     6181 aagctagcga agatagacag ttcgccttac catcttgtgc ttctgtgcga aaacaacgag
     6241 ggttatcaaa atcttataaa gcttgtgtca gcggcatata cagagggctt ttacagcaaa
     6301 ccgagagtag atgttgaatt gcttgaaaaa taccacaatg ggcttatctg tttgtcggct
     6361 tgtcttgcgg gtgagatacc cagaaagctt accaatggcg attatgaggc tgcaaagcag
     6421 actgctctta aatatcgtga tatatttggc agggataatt actttataga gcttcaaaat
     6481 cataagtttg cagaccagca gagaatattg cctatgctta taaagctttc acgagaaaca
     6541 ggaataccga tggtctgcac caatgacgca cactatctca gacgttcaga cgctaatgcg
     6601 caacgtgtgc taatgtgtat ctcaacaggc acgacactag acgacccaag caggcttgaa
     6661 tttccgacaa acgagtttta tgtaaagtca gcagaagaaa tggcggagct ttttccctct
     6721 cagcctgagt cgctggagaa tacagtaaag atagctgaca gatgtaatgt aagctttgag
     6781 tttggcaaaa caaagcttcc gtatttccat atagacggtg tttcagataa tgaaaagttt
     6841 cttcgtgata tgtgcatgaa agggctttat aaacgttatg aaaatcctac aaaagaggct
     6901 ttgaaacgtg ttgactatga gcttgacgtt ataacgagaa tgggatatac tgactattat
     6961 cttatagtat gggattttgt tcactatgca aagacccacg gcatacctgt tggctgcgga
     7021 cgtggttcag gcgcaggaag tctgtgtgct tattgcatag gcataacggg tatagacccg
     7081 ctgaaatatg acctgctttt tgaacgtttt ctcaatcccg aaagagtttc catgcctgac
     7141 ttcgatatag atttctgcat ggaaggcaga cagcgtgtta ttgattacgt tgtggagaaa
     7201 tacggctctg accacgttgc acagatagcg acttttggca cgcttgcggc aaagcaggca
     7261 gtgagagatg tggcaagagc aatgggtctg ccttatcaga caggagatat gcttgcaaag
     7321 aaaataccaa gaggacagcc acttaaatat tccatagaga atat
//
LOCUS       DAWEMR010000130         7241 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4195_length_7241_cov_9.983301, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000130 DAWEMR010000000
VERSION     DAWEMR010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7241)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4195_length_7241_cov_9.983301"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2022 a         1449 c         1887 g         1883 t
ORIGIN      
        1 gagagtattc ggaagagggc ctgcggcggc aacgctgttg tgtgaccttg caaagggtgt
       61 gcttgcggta gttatctgcc gccttgtggt gacaaacggt ttcggcgtga cattttttca
      121 tgacgataag tttatcggat atcttgcggg cttttttgta atgcttggac atattttccc
      181 ggtattctat ggttttcacg gtggaaaggg cgttcttatt gcggcaacga ctcttatcgc
      241 tatcgaccct ctcacctgtg ctttctcggt ggctgttttc gctatcgtgc ttgcggcaac
      301 aaaatacgtg tctgtgggtt ctatatgcgc cgctatcgca tatcctgttt ttacgcctat
      361 cgttcagctt tttatcatca atggcgacgg cgttcttgca aacactctta tggcaacggc
      421 tatcggtctg cttatcattt atatgcacag acctaatatc aaacgcctta tgaacggcac
      481 cgaaaacaaa ttcggaagca agaaaaaggc gtaagggagg ttttattatg gcagatatta
      541 ctatactcgg ttcaggcgga tttggtcttg cactggcaat aatgtgcgac aatgcaggac
      601 ataaagtaac agtatggtca aagtttcggg acgagataga tgctatcaag agaacaggcg
      661 agcttaaagc aaagctccct ggcgttcagg tgaacaagac tatcgagctt acaacggata
      721 taagctgtgt taaggataaa gacatggtga tacttggcat accttcacct ttcctcagag
      781 aggtctgtga aagcgttgtg ccttatcttg aaagcagaac gatagttgtg aacacggcaa
      841 agggtcttga gggcggaagc cttaaaacta tgtgctgtgt ggcgagagag tgctttccga
      901 acaattatgt tgcggtgctt accggtccgt cacacgcaga ggaagtggca agaaaagttc
      961 ccactactgt tgttactgct tctgacgaca gggaggtttc atattatata cagaacactc
     1021 tttctaacga tacgttcaga gtgtatgtta atgatgacgt tatgggctgc gagataggcg
     1081 gttcgctgaa aaacgtcatc gctttggcgg caggcgtttg cgacggcctg ggacttggtg
     1141 acaacacaaa ggctgcactt atgacaaggg gtatcagaga gatatcacgt cttggagttg
     1201 caatgggcgc aaagcttgaa acctttggcg gacttagcgg cataggcgac cttatcgtta
     1261 cctgctgttc aatgcacagc agaaaccgcc gtgcgggtat cctcataggt aagggagtta
     1321 cccctgagga ggctgtaaga caggtcggca ctgttgaggg ctatgtatgc accaaaaacg
     1381 catgggagct ttcaaagaga atgggaattg aaatgcctat cacagaacag cttgcaaagg
     1441 tgctttttga gggtgagcct gcggctaacg ctatcaaaaa tcttatgggc agaccgaaca
     1501 gaaatgaaac tgagtcaact tttctgagtt aggaaagcga ccgttgaaag gacggataca
     1561 tatgcttaaa agatacacta tcaagacaga acgcagaggg ctttacaaca tcacgagcta
     1621 tgtaaggcag gctgtcacag aatcaggagt gcaaagcggc attgctgtgg tattctgtcc
     1681 ccacaccacg gcggcgataa ccataaatga gaatgccgac cccgacgtaa agacggatct
     1741 tctttacgct ttggaggaaa catttcctaa cagaccgcag ttccgtcacg ctgaaggaaa
     1801 ctctgacgct catatgaagt caagctgtgt tggtgcttcg gaaactgtta tcatcgacga
     1861 taacgagctt cttctgggca cttggcaggg tatctatttc gctgagtttg acggaccgag
     1921 aacaagaaac ttttttgtga agataataaa gggctgattt tggcgttttt cctctttaca
     1981 atctgctgaa aatattgtat aattaatatg gataattcca acgaaatatg tgttaaggag
     2041 tgctttgaaa tatggaacct aaatataaga gaatattgct caaattaagc ggtgaggctc
     2101 tcgcaggcga taagaagata ggtcttgatt atgacgttat cacctcattt ggcaagagca
     2161 taaaaaaatg tgttgacgcc ggcgttgagg taggtatcgt tgtaggcggc ggcaatttct
     2221 ggagaggcag atcaagcggt gcaatggaca gaacaagagc cgaccatatc ggtatgctcg
     2281 caactgctat gaacgcactt gctgttgcgg acggccttga gaactgcggt cttgaagtga
     2341 gagtacagac agctatttct atgccacagg tggctgagcc ttacatcaga aacaaggcta
     2401 tgcgtcattt tgaaaagggc agagtagtta tcttcggctg cggcactggc aacccgttct
     2461 tctcaactga tacggctgct gcactgagag cggctgagat agaggctgac atattcttta
     2521 aggctaccat ggtagacggt gtttatgaca aggatcccca caagtatcct gacgctgtta
     2581 agtatgacac acttacattt gctgatatcc ttgcaaaggg tctgcaggta atggactcga
     2641 ccgctgcggc aatgtgtaag gacaacaata tcccgatact cgttttcgat cttgcaagac
     2701 cagacaatat ctatgacgct tgcatgggcg aaaatatagg cactctcgta aaataataaa
     2761 gataatcaag aaaggatcgt ttgaatatga aacagatcaa ggaaaatgct aagacaaaaa
     2821 tggagaaatc cataaacgtt atgctttcgg actttgcagg catcagagca ggcagagcta
     2881 atccgtccgt actcaacaaa gtccaggtag aatattatgg cgcaatgaca cctgttacac
     2941 agatggcggc ggtttctgtt gctgaggcaa gagtgcttgt tatacagcca tgggataagt
     3001 caactctcaa gcctatcgaa aaggctatcc aggcttctga tatcggcatc aatccacaga
     3061 atgacggctc tgttatcaga cttactttcc cacagctcac tgaggacaga cgtaaggaac
     3121 ttgttaagga catcaagaaa atgggcgaag agtgcaaggt agctatccgt tctatcagac
     3181 gtgacgcaat ggaaaagttc aaggcaatga agaagaacaa cgagatcact gaggacgacc
     3241 tcaaggactg cgaggacgag atccagaaac tgactgacaa gttcgttaag gaagttgacg
     3301 gtcacgttgc cgagaaggaa aaagagatac tctctatcta agttatggca aagaatgaga
     3361 aaataagcct tggggagaat gtttccgtgc ctgtacacgt tggcgttata atggacggca
     3421 acggcaggtg ggcgaaaaag aggggcttgc cccgaaagtt cggacacaga gagggcgcaa
     3481 agacctttcg tgccataaca agacacgcca aggcgcttgg aatacagtat ataacttact
     3541 atgcgttttc taccgagaac tggaaaaggc ctaaggacga ggtagacgca attatggatc
     3601 tttttgaaaa gtatcttgac gaggtcaggg actttatcga ggagaatatc agggtgcgct
     3661 ttataggcga cagaacaagg ctcagcgaaa atctgcaaag gaaaatggct tccgttgagg
     3721 aggactcaaa ggactttgac tctatgaccc ttgtgcttgc tatcaactac ggcggcaggg
     3781 acgagatttg ccatgcggct aagaaattgg ctcagcaggt gaaagaaggc aagctggagc
     3841 ctgaggatat cacaatggat atgatagaga gaaatctcta cactgaggag atacctcctg
     3901 ttgaccttgt tataagacct agcggagagc aaagattgtc aaactttatg atatggcagg
     3961 cggcttacgc ggagttttat tacacaaata tcctatggcc tgacttcaaa ggcagcgacc
     4021 ttgacaaggc tgttatcgca tacagcgaaa gaaacagaag atttggaggg gtgtaaaaaa
     4081 tgtctacccg tattaaatct gcggcagttg ccattgttat cgcagtgata ctgcttatcg
     4141 cacacggaac gttcctgttc aacattgcgg tgggcgcaat ttctgcactt gcaatatatg
     4201 agatattcag ggcgacaaaa atgacagagc ataagtatca gaccattgcc tgctgtgcgt
     4261 ttgcctttgt ggacgctttc atgcctgtgt tctacagaca cggctggctg tactttttca
     4321 gctacaagct ttacatgggg cttttcgtta tagcaatgtg tctgctttat cttaaagagc
     4381 atacaaaatt ccagtataca gagtttttct ttatgctggg cgtgggcata cttctgccgt
     4441 attcattctc aactttggtc acaatggcag gcttgggcgg caagggcgtg tttatgatag
     4501 ttcttactct tgcagcggct tggctggctg acagcggtgc ttattttgca ggaactttct
     4561 tcggcaaaac aaagctttgc cctgagataa gccctaagaa aactgtggag ggtcttatag
     4621 gcggcgttat ctcaaatggt attcttatgc ttataataag cctgttctat cagttcgtac
     4681 ttaaaggcga gcctatccac tatctaggtg ttctaatcgc aggtatgctt gcggctctta
     4741 tcggacttgt gggcgacctt acagcttcgg tgataaaaag acagacaggt ataaaggact
     4801 atggcaacat aatgccgggt cacggtggaa taatggatcg ctttgacagc gttctgctcg
     4861 ttgcaccttt tatgtactat atgttcacac aggggcttat acttaaatag aaaattatga
     4921 cttttagggg caggcttagg cttacccctt ttaagcgtta ttaaggcaac accgcttgca
     4981 aaccatcttt atgcggaatt gcctgaattt attttatatg atgagggaca ataaatgaaa
     5041 acaatgacca tactcggttc aacgggttct ataggcacgc aggcattaga agtggctgca
     5101 aagcataata ttaaagtaaa ggcgcttgcg gcaaattctg acgttgaaaa gcttgcggct
     5161 caggctaagg agcttttgcc tgagcgtgtg tgtatctacc gtgaggacaa aaaggcggag
     5221 cttgaaaagc ttctcagtgg cactggcata aaaatatcca caggcatgga gggacttaaa
     5281 gagatagcaa gaatggacgg ggtcgatatc atgctcaact cagtcgttgg catggtgggt
     5341 cttgtgccaa cccttacggc tatcgaaaaa ggcactgaca ttgcccttgc aaataaggaa
     5401 acacttgtgg caggcggtga gcttgttata aaggcggcgg ctgaaaaggg agtgaagata
     5461 tatcctgttg acagcgaaca ttcggctatc tttcagtgcc tgcagggaaa cgagggcaac
     5521 aagctcaaag ggataatcct tactgcttcg ggcggaccat tcttcggcaa aacaaagtct
     5581 gacctagagg gcgtgacagt tgagcaggct cttaatcacc ctaattggtc aatgggcaga
     5641 aagataacca ttgactctgc tacgcttatg aacaagggtc ttgaatttat cgaagcaatg
     5701 tggcttttca agctcagacc tgagcagatc gagatagttg tccatagaca gagtgttgta
     5761 cattcagcgg tggagtatga ggataattct gttatcgctc agcttggcgt gcctgatatg
     5821 aaaataccca tacagtatgc tctgctttat cctgagagag tggaatgtga tgtgaaaaag
     5881 ctgtccctta cagactatgg aaagctcact ttcgagaagc ctgactatga tacgttcgat
     5941 tgtctgaggg cttgtataaa ggctgtcgct atcggtggaa atcttcctgc ggcagtgaat
     6001 ggagcgaatg aggaagccgt tgcggctttt cttgacggca ggatagattt tcttgacatt
     6061 ggtagaatag taatgagcgt ttgcgacagc accccacgaa aagagataaa atgtgtggaa
     6121 gatgtgctgg aagctgacag actggctagg gaagaggctc gcagactggc tttggcaatg
     6181 aaaaattaag ggatagtttg cggtgctgaa cctattgtct ttttgtgttt gaaaggaaag
     6241 aaatgagtat attatttgca atattgatct ttgaagctat cataataata cacgagcttg
     6301 gtcactttat tgcggcgaaa gccagcggag taaaggtcaa cgagttcgct attggcatgg
     6361 gtcctgccat tatcaaaaag aaaaagggcg atacccttta cgcttggcgt gtgtttccca
     6421 taggtggcta ttgtgccatg gagggcgagg ataacggaag cgaggacggc ggtgcgtttt
     6481 gtaataaaag cctgccaaaa agaatgctta tcgtggtggc aggcgttata atgaatcttg
     6541 tgcttggata tattaccctt tgtatcagca ctgcaaggtc tgacggcatc gcaaccacca
     6601 ctatatcgtg gtttgaggat aacgctatgt cccaatcgac cggcttgcag gtgggtgacg
     6661 agataatcga ggtcaacggc atgaggattt tcactactat ggatatgtcc tatcagtttg
     6721 gcaacgatga ggacggcaag ttcgatatgg tggtgaagcg tgacggaaaa aaggtggagc
     6781 ttaaagatgt cacctttgca acggacgata ataccatgca catcgatttc aaggtcgcac
     6841 caaaagctgt gaccgtcggc tctgtgataa ctcagtcttt taagcagggc tgtactgacg
     6901 gcagacttat ctatatctcc tttgcagacc ttataagagg aaagtattcc ataaaagacc
     6961 tcagcggtcc tgtgggtatc gttgacacta tcgacaatgt tatcgacagc gaaagggata
     7021 gccagtcagg taaaatagat tggtcgtcac ttattgacac aatgttgtcg ctgggtgcgt
     7081 tcatgactat aaatatcggc attttcaatc tcttgccatt gcctgcactt gacggcggaa
     7141 gacttttgtt tcttattatc gaagcggtac gccgtaagcc tgtcaagcct gagcatgagg
     7201 gaatggtaca cgctatcggt atggcggctc tgctcctgct t
//
LOCUS       DAWEMR010000131         7226 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4205_length_7226_cov_16.887184, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000131 DAWEMR010000000
VERSION     DAWEMR010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4205_length_7226_cov_16.887184"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3856..3865
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1706 a         1582 c         1777 g         2151 t
ORIGIN      
        1 tcaggatagg gggcgcaggc tcggtattct ggatgtgggt gtctgcattt ttcggaatgg
       61 ggcttgctta ctgcgaaaac tatctgggag tgagatgctc tgacggaaag cattgcggtg
      121 cggcgtctta tctcgaaaag ataggcagaa cgaagctcac ggctgttttg tttgcagttt
      181 ttacggtggg tgcgtccctt ggaatgggca acatggtgca gacgggttcg gcggctgcgg
      241 ctgcccgaga ggggtttggg ataccctcat atatatttgc acttgtggtg cttattttcg
      301 tatttttagt agctgtggga aaaaactcag cagcttgtct ttgcgaaaag cttgtgccga
      361 taatggcggt gcttttcata ggcggcagtt tggaagtgat aatatttcac ccattaaaag
      421 ccgcaggagc gatagcaatg atagtaaaaa acgccttttg ccccacagcc tgcataacag
      481 gctcggtggc agggacgata atattttcca tggtggaggg gttaaagcgt ggagcgtttt
      541 caaacgaagc aggcttaggc tcgacggtgg cagtacacag ctcatgcagg ataaagtcgc
      601 ctgaattgca gggaatatgg gcaatggctg aggtttttat agataccttt gtcatatgca
      661 ctctgacggc tgtggtgata ataataagcg gcactgacct cacgggggag gatatggcag
      721 tgcgggctta caatgctgct cttggtggag cggggaagta tttcatgtca ggttcgctga
      781 tactttttgc attagcgaca gtggcagggt ggtttttcat aggggagagg gcttggagat
      841 accttttccc tcgaagtgat atgatctata ggatcatggc gatagcttgt gggtgcattg
      901 gaacgatgtg gagcatggag cttgtgtggg gagtgtcgga tattttcaat ggacttatgg
      961 cagtgccgaa tgttatcggg gtgttggtgt tgtggagaaa agaggtagaa atgaggaatg
     1021 aggaatgagg agtgaggaat tggcaaagct gaccagagtt gtgcacaagc acaaagtaaa
     1081 agttagcacc gagcgaagcg attttgcggc acgatgaagt tgtaggggac gacgccctcg
     1141 gcgtcccgtt atcgaagtag tgagttttgt tttttcgtga agtcattcaa acgaacaaag
     1201 gcgggtgaac accgttcgct cccacattat gcccttatat ctctccgctt gcgtgtcttg
     1261 tcacatgaca gcctacgttt actgacaccg gcaggcctgc gatatgggtc ggtgcttctt
     1321 ctatatttac tgcaaggcaa gtctgtgtgc cgccaaagcc ttgtggtcct atgccaagga
     1381 ggtttatctt ctccagcatt ttcgcttcaa gctttgcata acgttccttt gggtttctct
     1441 ttgatacgct tctgcacagt gctttctttg caaggtatgc gcacttctca aagtcgccgc
     1501 ctatgcctac gcctatgact attggcggac atgggttaga acctgctttt gcacacactg
     1561 acactaccca atccactatc tcgtcctcgg taactgacgg ggtcatcatt ttaagctgtg
     1621 acatattttc gctgccaaag ccctttggtg ccaccattat ttctaccttt tcgccctctg
     1681 ctattttaag atgcacaaca ggcggtgtat tatcccctgt gtttactctt tccaatgggt
     1741 ctgatactat ggaacagcgc aatctgccct ctgtatagcc tcttgctaca cctttattta
     1801 tggcttcatt cagcgaaccg cctacaaaat gcacgtcctg tcctatggtc atgaagataa
     1861 ctgccatgcc tgtgtcctga cagataggga tagtttcttc tcttgctact ctcatattgt
     1921 caaggatgtc agcaaagacg ttctttccca ccggggactg ctctttcctg gcgttgcact
     1981 ctatacagct ttccatatcc tcaggaagat acagattggc ttttatgcac agatctctta
     2041 caagttcttc tacttctgaa acgtttatct ctctcatttt tatttatcct ttcgctctgt
     2101 atttgctacc ttttcacggt cgctgagctt tattactttt tcgctgtcaa ggacatagca
     2161 tggcttgccc tccagaagaa attctccttt tagaaaatct gcgcaggctt cttcgttgac
     2221 gtttggggct ttacacagct tatcctcggg aatatccgtg aagcctgtta cgctgtcgca
     2281 caaaagccct acggaaactt cttcgcccat tgtgattatt atgcaatctc tgtcggagat
     2341 ctcttttggc tcatagtgga aacgtctgcg caggtctatg acaggcacta cctcgccctc
     2401 gttcaccact gttcccggca catagtcggg aaaatcaggc acgggagttg gggtctgcgc
     2461 cgctatgatt atttttatat cctcaaaggg cagtgaaaag cttcgccctg ctgaggtgaa
     2521 aatgagatat ctgctgtttt ccacagctat ccctccttgt tatattttat cataagccgc
     2581 tgtcgatgcg ataatgtatc tcctgtgcta tcttgtcaag gggacactgc tttgacactg
     2641 agccgttttc atatgcggct ttgggcatac catagactat ggagctttct gcgtcctgtc
     2701 cgatattata tgctcccatt ttcttcatgt tgagcatacc ctttgcaccg tcacagccca
     2761 tgccggtgag gataacgcct atagcgttac gctttgcgct gtatgccaca gagtcaaaaa
     2821 gcacgtcaac tgacggacag tgacctgaaa cctttacacc tgcttcagag gtgacaaaat
     2881 agcctttctt atccctgaac aatctaaggt gctgaccgcc ctgcgctatt accaccatgc
     2941 cttgactgag atacagaccg cttttggctt ccacaacgtt gtgaccggtt tcagtgcata
     3001 gctgttcggc atatatcctt gtatagcccg caggcatatg caggactgcc actatagggg
     3061 gcaggtcgtg gcggaggtct ttaaggatag ctttcagggc ttccgttgag cctgttgaac
     3121 cgcctatggc tattattctg gaggtattta tggcaggtat atgcacaaca gaattagctc
     3181 ttgctttttc aaggcgctgt atgctttcgg caaagcgttt ctgaaaacgc tcgttatcag
     3241 acggagtgcc gtcaggttta tatacgacgt ctatggcacc tgcctgcatc atgggatatt
     3301 tattgttatg ctgaatggta agatagatta taggcacaga atatcgtggg gcaaggctta
     3361 tgatgtagtc gtccatattg tcttggcaat cgccgtcgag aagaacatag tcgggagaaa
     3421 gggcggtgac gctttttctg ccgttgctaa ggcaggctga ggcaagcttt gagctttgga
     3481 agcctgcgtt tgtaagggcc tttctgcaaa gctgtgcaaa ggctttgttt tcggttatca
     3541 caagaaagag aggcgtcatt ttttatcccc tcccagcttg cggtatattg caggggcaac
     3601 ttttacaaaa ggcatatact tcggcactga ttctgcgtga ccgatataga aatagccgcc
     3661 atcctccatt gcgtcataga attttctgca tatctcggct tttgtatcgt cgtcaaaata
     3721 tatcatgacg ttacggcaga cgataaggtc gaatttcttt ttgaaagtga aatcatccat
     3781 aagattgtgg taacgaaact caacattctg ccttattgta tcgctgacct gccagagatc
     3841 ctttgtgttt gaatannnnn nnnnngtttg aatatgggtg aaaatatttt ttacgccaat
     3901 cttcggggat attctttatg gacatactgg tataaatgcc gtttttggca gaacgcagtg
     3961 cgttaaggga gatatccgtg gcaagtattt tcaagtccca gaaacggcga tcaaagccga
     4021 aataatcgtc catacacatg gcaagattat atggctcgtt gccgaatgag cagcctgcac
     4081 tccatatacg catatcatga tcgaggacgt ttttttcggc ataggggaga aatttctcca
     4141 tgaagtgttg gaaatggtcc gtttcacgca tgaaatatgt atggttggta gtaaggcggt
     4201 ttataaggtt ggcagtttct ctgcctgttt catcgctgta gacggcgctg agatattctg
     4261 aaaagctgtt gaagccgcag tccaatacat agttgctcag cctgctttca acgatagact
     4321 ttttccttgc aaggtcaaga ccgtattttt cttttatgaa tgcagtaagc tcgtcaaagt
     4381 ctgactgaga gatctcaaga cgtgacagca ttcctcttcc tccttgttat tatgttttac
     4441 ctgtttgttc tgaccagaat atcagggtca agcagggata tgtttttgtt gtttgcgttt
     4501 ataaagcatt ttacaaggct gttttttacg ggcgacacgg cagtctgctg ggggaggata
     4561 tcctcaacgt ccaagacctt atcgcatatt atgccagcct gatttgagcc gacttctatc
     4621 actatggtac tgcttctgtt gtcatactcc ccttcgggca gtccaaggcg caggcggaga
     4681 tctatcacgg gaacggcttt tcctcggaga tttataacgc cttttatgaa ctttggtgtt
     4741 ttcgggagcc ttgtgacggg gagaagccct attatatcgg tgacatcttt tacctcaaag
     4801 gcgtaatatt tttcatcaga aacaaaggag agtatttttc cgcccttctg acaaaattcg
     4861 tttatatcct gttctgacat ctttattcac cctttcggaa gtcataattg ccgagaaatt
     4921 ctttcatatc cagtattatg gttatactgc cgtcaccgag gaccgaacag cctgccatgc
     4981 ctgattcctt gaggggatag gcgtcaagga gaggtgagaa cggttttact accacctgct
     5041 ggtcaactgt tatcctgtct gccatgagga cggcttcctt gccgccgtct ttacaacaga
     5101 gcattatgcc ctttgttacg tccttttcgg catttggtat atcaaaatgg tcgctcattc
     5161 tgataagagg cagacatttt tcacggagca ttataaactc gttgccctcg gggtcggtga
     5221 taaagctttc ggctttgcag gagaaagctt cggaaactgc ggaaacaggc agagagaaat
     5281 tctcgccccc tacgtcaacg cttaaaacgt caaggataga cagagaaagt ggtatcctta
     5341 tagtaaagac agagccttcg ccaaacttgg agtcaacata gagctttccg ccgactttct
     5401 caaggttttt ctttacaacg tccattccca cgcctctgcc tgaatattcg gttattttat
     5461 cattggtgga aaagcctgcc gccacaacaa aattgaatat ctctttgtcg gtatattcat
     5521 tctcaggctt cgtgagcata ccgtttttct tggctttcgc cataagcttt gcggtatcca
     5581 ttcctgcacc gttgtcacgg caggagataa ccacttcgtt ggagtcatag cctgcactta
     5641 gagtaactgt ggcaggctcg gtcttgcctg cttttgcacg gacttcgggg tcttctatgc
     5701 cgtggtcaac tgcgtttctc acaatgtgca taagggggtc attgaggata tctacgatag
     5761 atttatccac ttccgtttcc tgcccttcaa acacaagagt aacgttcttt ttgagctttt
     5821 ggttcatatc cctgacaaca cggttcatct tctgaaatgc tgtggaaaca ggcaccattc
     5881 gtatggacat tacaacatcc tgaagctcat cggtaagctt tttcagctca cgggaggatt
     5941 tattgaagcg gtcgaggttt atctcggctt ttttcaggtc gggagaggag ataacagaac
     6001 cttcggttat aacgatctca gccacaaggt caagaagtct gttgagctta tcgagcttga
     6061 cggatatcat ggactgctca tgcttttcgg catgcttttt ggggactgca tttgcaggct
     6121 gagcaggctt tggggctgac ttttctggag cagacttttg ggcaggctga ggttttttgg
     6181 ctgcgttagc aggttgttca ggctttttcg cctgctcggc tttgtcaacg ttcacggcgt
     6241 ttttcataag gtcaagaacg ccattctggt catcagtgat aagctttatg taaagtccct
     6301 ttttggatat ctcgtctcct gcattgtcgg cgtcaagatc ctcagggaaa gtttttatta
     6361 cctcgcacat tttacctaaa gcacgcaaaa gcaccaatgc acgcatatca ggcattgcac
     6421 agctttcgga atatgtgatt ttataggtta gaacgccttc tttttcatca tcagggaaaa
     6481 gcccctcata ggtgttggta tcggctgagg aagtatcgga actgtctgtg gcaggctgac
     6541 ctttcatggt ggcagcaaag gcgtgtatct tattttcaag ctctgaaaaa tcggtgagcg
     6601 gtgcgtcgtc atagagtgaa tcaagctctg atttaaggtt atcggaggct gagaaaagaa
     6661 gctcatagag agcaggcttg tcatatttca cagtagggtc ttcacggata atgtagaaga
     6721 gatcctcaac tgagtgggca agggtagaca tattgtgcat actcatcatg gcggcagagc
     6781 ctttaatagt gtgcatggta cggaatatct cgttgatatc atcggaggag atagagtctt
     6841 cctgttcggt gcgcataagg atctcgtcca acttttccag aaggtcgcct gtttcaaaga
     6901 cgaacatatc cgccatattt tccataccgc tttcaaattt cggcaaaaaa gtcactccct
     6961 ttcggtgctg ttgaagcata cggatataat caataatatt atagcaaatt ttttgatatt
     7021 tttcaagcat ttttgtgcat tttaagtaaa agaaacgatc aagtgaggag ttaggaatta
     7081 ggaatgagga attaggaatg aggaatgagc aaagctgccc ccaagcaccg agcgaagcga
     7141 tagataaagc gcacgctcaa gctcggcact ggagggcttg tggggtctag gggcaaagcc
     7201 cctagcggag tttgaggcag agcctc
//
LOCUS       DAWEMR010000132         7189 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4228_length_7189_cov_13.450659, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000132 DAWEMR010000000
VERSION     DAWEMR010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4228_length_7189_cov_13.450659"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6315..6338
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1798 a         1871 c         1331 g         2165 t
ORIGIN      
        1 cttgcttttt ggcaagatcg cagccttttt tcttggggac tttttttaca gccccctttt
       61 ttgttttccg cacagaccaa ctgtaggggc gaacagtgtt cgcccgtttc attttttcac
      121 gatcctactg attattacat tgtataaaca ctgcacccaa atgtagggga cggcgccccc
      181 gacgtcccga catcgaagcg ttaaggtttg atttatcata aagctgtgta ggcaaacaaa
      241 agcgggtcag agctttacac tccgacaccg ctttattata gcttattatt catatctcaa
      301 agcgtcgata ggattgagct tcgccgcctt tatggacgga ataagtccaa atactattcc
      361 aactaccatt gaaaatacca ccgacactac tattgatgcc gaggatatcg ccacaggcac
      421 ttccgctacc ttcgctatta cctgcgaaag ccctatgccc gtcagcacac cgattattcc
      481 gcctatgctc gtaagcactg ccgcttctgt caaaaactgt gcaagaattc tctttttcct
      541 tgctcccagt gcttttttca gtcctatctc tctcgttctc tctgttaccg acacaagcat
      601 aatgttcatt acgcctatac cgcctaccag cagtgagatg ctagctatcc atataagcat
      661 cttattagtc gatgaactca gatcctgcaa tgactttgcc tgctccagca ggctatcgct
      721 cttgtattcc acagaagcgc tggtttcctt acttccgccc tttacagccg aaacgttctc
      781 gttaagaata tccgccgact ttttgccagc attcgtcatc gagtccgtgt ccacagcctt
      841 tacgatacag ttttctggct cgtcatagcg gaaaactatc ccccaatcgt ttgtggggat
      901 aaagacaagt ccgcttgatg tctgattgta tgtgtagtag tcctccacgg agcttataac
      961 aggctcaaag tttgaatttt tcacagccac gcctattaca gtgaacggct cgccacttat
     1021 atctatgacc tttcccacag ggctttcacc ctggaaaaga ccctctgcaa gcgtcttgtc
     1081 taccactgcc accttgtcaa atttggtaaa gtttctctcc gcaaagccct ttccctcaac
     1141 tatctcgtat ccaacagtgt caaaataatc cttgtctact ccatatatag ttgacgaatc
     1201 cattttgttg ttaagataaa acagattgtc aggattgttt cttgtgcggt aaaaagtgct
     1261 tgactccacc tctttaagtt cgttgatacg ttttttagtg tccggtgtga acattgccac
     1321 attagacggt ggcgcttcat aagaaaagtc gcaaacagtg tcgccatttg aaagctggat
     1381 cttcaccgtg ttgtttcctg cgccgataag attgttcttt atctgctcat ttgtaccctt
     1441 gatagtggaa actatagcaa tgatagccgc aataccgata atgataccaa gcatagtaag
     1501 aaacgagcgt atcttatgtg aaagtattcc cttaaatgaa agtctgatat tttctatcat
     1561 attatccgcc cccttttaat actcaggata aaggactttg tcggtctcct ttgtccttac
     1621 gccctctttg acgtccttgc catatgggaa gcatatctta tctttcatag aaagtccgcc
     1681 cttgacctcg atattagtgc catacattat cttgcctgtt tttatgtagc ttttcacaag
     1741 tctgccgtcc ttgttggctt tcattatata gtagtttccg ttttcctgcc ttacataatg
     1801 tataggcaga taaacgctgt cactggtcgt ggtgtcattc acgaatgaaa cgcctaccca
     1861 gtcgcccacg ttgaaatccg tgctgtcgct gagcttagct tctatagtgt aggtggagct
     1921 gttaggattg tcaccccagt tgtttgacga gtatgacgta ggctcgctgc ttatctttgt
     1981 tatctcagct tcaatgctca ttccagtgtt gtatgagttc accataacag tgtttcccac
     2041 agccgctctg tcaagattaa gctcgcccac actaaaagta acggaaactc cgccctcgcc
     2101 ctcgattatt gcgaaaacgt catcatcaga gctgtcacca ctgtagtccg tgctgtctgt
     2161 aatgtcctta tcgtctttgt cgtcaccatc gtccgaatta tcaccctctg cggcagcgtc
     2221 gcctacagtg cctatctttt taacgactcc gtcgatatta gccactacct tgccgtctgt
     2281 ctttttcttc aaagcgtcat tgtagtcaag ctttgcagac ttaaggttaa gctttaagct
     2341 ctttatctcg tcctcttttt ctgatatcat tttctttatc tctgcacggc tgtgcatata
     2401 gtcctcagag cctgaatttg taccgcccgt ctgtgacggg tcagcaggag tgacatctat
     2461 gccagcgtca ttatcatcat aatcaccgtt atcaggtata tccacatcac cgccattgtc
     2521 gatatcatca gtatcatcgc tatcgtcagc actgtcatca ctgcggtctg agcctgcagg
     2581 cacaacgata gagaacttgc cgaactgctc cacagaagtg tcaagagaaa gcataccgct
     2641 ttccttgtcc tcagttatcc cctgcgaaac tgtccagtct gcaagagcct ttttgcccat
     2701 tgtttcacgg ttgactatcc acttgtatat aaagtttgag ttatcatcat aaacgcaaag
     2761 ctccgcataa gcccccgttg tctttatcct ttcaagaaaa gctctggata cctttgtgtt
     2821 cctgttgcag tttataatgt aaggatcgtc ctttgtgcct gcatttttcg tctggaaaac
     2881 agcctttaca acgtccacag tttccacagg ctcaggagtt ggctcaggat ctggagttgg
     2941 ctctggctct ggttccggct ctggctccgg ctcaggctct ggatcgtcag gctcttgagg
     3001 tgcgtcctca gaaggcttta aaagcttgta tttagcaagc tccttttcag ccattttgat
     3061 gttctgctcg cagaccttta cctgattttc tttctgtgaa agctccagct ccaccaccgt
     3121 catgtcatat tcaagtatag tgtcgccctt tttcacagtg tcgccctttt tcacaaacac
     3181 ctttttaaca agcttctcgt tgtcaagagt aattctctga gcattggcag aaactatctg
     3241 cccgtccatg ttcgttgagc tgtcccactc gtacgcatcc gcagtttccg ccataaggtc
     3301 aacaggaact accgacacaa cagttttctt gtcctgattt ttcttatatt tcactgctcc
     3361 cgcatatccg cctccgccga ggatagctat gcacaggagt gttattatta cctttttcat
     3421 tcctgctcac ctgccttttc tccatgaagc tcgccgtcac ggatatacat tatcctcttt
     3481 gcacatcttg caatgtcagg ctcgtgtgtt atcataacga tagtaacacc gtcatcatta
     3541 agctccttga aaagctccat tacctgatcg cccgactttg tgtcaagagc gcctgtgggt
     3601 tcgtctgcaa gaagaagctt tggattgttg actatcgccc ttgcaatagc caccctctgc
     3661 ttctgaccgc ctgaaagctg agtaggcttg aacatcattc tctgtgcaag ccccaccttt
     3721 tcaagagccg cctttgctct ctgacgtctt tctcttttgc ctacccccgc ataaagcagc
     3781 ggaagctcca cattttcaag agccgattgt ctagggagca gattaaagtt ctgaaataca
     3841 aaacctatct tcttgtttct tatctccgaa agcttcctat cctttgcctg cataatgtca
     3901 cagccgtcaa aaacaaagct tccctttgtc tgcttgtcaa gacagcctat aatgttcata
     3961 agcgtagact ttcccgagcc tgacggaccc attatcgcca catattcccc ctctgagaca
     4021 tcaagggaaa tatccttaag cacaggcaca gcttccttgc cctgcaaata atccttgtag
     4081 atatttttca gttcaaatac cattgcaagc cctccttatt cagcgaaagc tgcgtcagca
     4141 tcagcagcgt cggcattctc accgtctgca ttttctgcat tttcctcttc accgtaaagc
     4201 ttgttgaagt attcttctat ctcctcgtcc gtcatgcttg caagcatatc ctcgtctata
     4261 gccatgccgt cctcatcact tccgtcaaaa gcgatgtcct cgtcaccgcc ctcggtatca
     4321 gcgtcactgt tgtcgttcgt gtcaaggtca ttaggtggaa cagacacgtc agcctcattt
     4381 gtggtagccg tcatgccctc ctcgatagat ttgtcagggt atgcgatata atcgcccttt
     4441 tcaagacctg actttatctc gcagtcaccg ttatcctcgt ctttctgacc tatctcaaca
     4501 tatctctttt ctatctttcc gtccttgtcc ttagcccaaa cgtaattctt cttgccgtcc
     4561 ttgcagataa agtcggaata gagccatatg cccgtcttgt ttatagaagt gtcctgtccg
     4621 ttgtctatct cgataagaac gtgctgaccg agcataagtc cgtcaaagct ctcaggctta
     4681 acatagaaag catagtttga agactggctc atctcatcgc ttgaaccgaa gctgtatata
     4741 tcatcaccag aatttttctg cggacttgtg tcaacagaag ttatctcgcc cttccaagtg
     4801 gaatcatcaa gccttgactt tacgataaca gaaacaccct gcatgatatc tttgctgttc
     4861 tgctcgttga atctgccctt tattctgtag tcgccctctt ctgttatctg cattataacg
     4921 tcgccgccgt cctgctgaag cttgtccacc gtcttgacgt tctttacagt tccgtcgata
     4981 gaagcggtga cgtatgcgtt ctttgtggaa gcctccagct ttttgatctc aacgttttta
     5041 gcctttatat catattcgtt cttagcattg tcgctctgca aagaaaggat ctgggtcgtg
     5101 tatgacacag cagcgtcctg atcggcgttt ttcttttcct tttcaagctc agctatctgg
     5161 ctcttatttg aagctatctc gttctggagc ctctccacct cgagcttagc ctgctcaagc
     5221 tgaagattta tagcctcaac gtcatatgta agaagcttgt cacccttttt aacagagtca
     5281 ccgttcttaa caagtatctc ggccaccgtc tttgagctgt catactttac gtccacagac
     5341 tcctgtgcct caacaacgcc caagaacgag cgattagaaa gcttgaagtc cgacgcagtg
     5401 ttaagtgacg ataccttctg cacatatacc ttctgtgagc tttttgagcc tcccttgccg
     5461 gtctttttcc atacgaaaaa gcctcccaca cacactgtgc agacgataac aagcactatt
     5521 ataacagtgc atatcctttt tttcgtggtc attgcaatac ctccgtttgt gattttttcc
     5581 aaaacagcat aaaaaaatcc gcctgacagc agccagacgg ataaaacagc cccaccttcc
     5641 ccataagggt atagcatgag cgtattattc tttgttatct tgctgtcctg ttgtcttttg
     5701 ttcttatctg tttttatgta tacattatac cataaaaaga atacaagtca ataaaaaaag
     5761 acgttgtttt aagaaactca taagaatttc cctcatctat aaagacgtac aataacaaat
     5821 aaagttgcaa acaaaaaaaa tctctctcgt actatcccgc agaggcacct gatggttttc
     5881 aacactttcg gtgcaaacag cattcaccaa aacagattgt aacaaatcac cccaacaaaa
     5941 aagggcagcc cacgccgccc taatatgtat tattttttct tagccataag ccctaacatt
     6001 ataagcacta ccaccgcacc gccaccgata cagccgaaaa gcacccaatc ggtctcagtg
     6061 ttctgggtgg agtttttctg ctccttgatg accccctgct ccgccatagc ctccgccgca
     6121 gagctttccg tcacaatgga gctgtcctcc gtctgagggc tgtccgcata caccgcccac
     6181 ggtattatca tcataagcga catcacagtc gccccaagcc ccgacaaaag ctttctcgtc
     6241 ctcatataaa aagacctcct ccgatacgcc atttatcaac agcaaagctt tatcacagag
     6301 ctgctttaag ttttnnnnnn nnnnnnnnnn nnnnnnnnaa atcatacatt agtttacaca
     6361 acaatataca caacacctca cgaattacaa ataaatcgct gtaaattttg atttatcagt
     6421 ttattaattg tgtattgtca caaaaatata tagcaaaatt catcaaaaag agcattgaaa
     6481 atcttgccca tatgtgatat aataacgaat agcaacatac tgttcacact ttacttgtaa
     6541 ggaggtcttt attttggaag aacgtatgca aattatacag gcagcaggca acagcaagat
     6601 acagataggc gttataccgg gtcactatgc aactaaccac tcgcatatca attattatgt
     6661 agatataaca tctcttaaaa ctggttataa aatggcaaag gaagccgcaa aggcattggc
     6721 agccacctat gtttcaacac atatcgacac tatcatatgc cttgaaggca cagagatcat
     6781 cggtgccttt ctcgctgaaa gccttggcga aagcggcata aacagcggtg cagacgtaaa
     6841 tgtagtaaca cctgagctaa acgctgtaaa tcagatgata ttcagagata acactcagaa
     6901 gaagatatgg ggcaagcagg tgcttctgct tatcgcttcc gtttcaactg gaaagagcat
     6961 aaacagagca atggactgtc tgaaatacta taacggcaac cttgtaggaa tagcttccat
     7021 attctccgct atcaaagaga acaacggcac tgctatccat gcccttttca cagaaaagga
     7081 tctgcctgat tacatgacat atacatcatc agaatgtcca atgtgcaaat caggacagaa
     7141 ggtcgacgct ttggtaaaca gcttcggcta ttcaaagata tagcttgta
//
LOCUS       DAWEMR010000133         7151 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4253_length_7151_cov_11.058343, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000133 DAWEMR010000000
VERSION     DAWEMR010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4253_length_7151_cov_11.058343"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    834..843
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1546..1555
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1912 a         1817 c         1245 g         2157 t
ORIGIN      
        1 gctttttggc aagatcgcag ccttttttct tggggacttt ttttacagcc ccttcatcat
       61 attctcttat cagccaaatt caaatccgcc agccctcact ctgtcgataa agtccccgag
      121 aatatcagta ttggttttcg atactgagtg aagcaaataa atagccccag ggtgagccgc
      181 attcacaagc ttttcaagtg cctgcccctc atcacgctga ttgttcacgt cccaatcagc
      241 gtaagcaaag ctccaaagct cagtcttata accgcagttt gccgtcactc caagagagaa
      301 ctccgaaaac tcacccatag gcggtctgaa cactgtcatg gtataatcat agttatcctc
      361 catatactta tggaaatcct ttatctccga ctcacattcc gcctgtccaa gcgtcggcat
      421 agaataatgg tgtaccgaat gatttcccac aatatgcccc tcgtctatca tacgcttcac
      481 aagctcagga tttttctctg cataatcctg caaaagaaaa aacgtcgcct tgaccttttt
      541 ctctttcagc acatcgagga tcttttcagt gtatccattc tcataaccct gatcaaaggt
      601 cagatatatc ttgtcatcat tatttatagc ctttgcgtta agcttgccgt attgtgcatt
      661 aaaatcaagc gcaccagtcg tcctgttctt ttcgtccaca agaacgcctt gaccatatcc
      721 gactttgtca tgtgaaagcc ccgaaagatc tatcttttcc tttgcggttt tctttcccac
      781 agccacaggc tcgatgaccg ccgagcttgt tccctctgat gaatttccct cacnnnnnnn
      841 nnnctcacgc attatcactg tcctgccaca agcactgagc gctacaactg cacaaataag
      901 tgccatgcaa tttcttatct tcatatgaag ccctccgttt tcttatcatc ttatactatt
      961 atgttcagaa aacggcttta tacaaaaata cgtttcaggt aaattctgac gtaaaacaaa
     1021 aaaggcggta aaaactaccg cctttaaata tcagttatga ctcaagatag gacttcacca
     1081 atgcctgcaa aagttcatct ctgtcaatat gacgaataag acttctggca ttattgtatg
     1141 ttgttatgta ggtcgggtcc tcagaaagaa tataacctac gatctgatta ataggattat
     1201 agcccttctc tttcagagcg tcatatataa ttgtaagtgt ctttttcagc tcagcctcct
     1261 tgtcgtgctg tactgaaaac tccatagttt gttcgtgcat tatgctcagc ccctctgcaa
     1321 tgtattaata tcagcaatac gcctgatatt attatactcc atttgtttca aaataacaag
     1381 tccaaaagca aaatttatgc acattataca aatcaagcac tatatttttt acattctgag
     1441 ttcagcaccg atagccttga gagcttcaag aactttcttc atagccttgt cagcctgctc
     1501 gtcagtaagc gtgccgtcat gtgaacgcat tgaaatggag tatgannnnn nnnnnatgaa
     1561 acagacttct tgcccttttc gatctgctcg cccttgtaaa cgtcaaaaag cgttaccttt
     1621 tcaagtatcg agccaacagc cttgcttata gtcttttcaa gaagagccgc agggatatcg
     1681 tcatcacaaa cgatagacag atctcttgtt gtagcaggat acttaggcag tggcttatat
     1741 gtcttctgag caacacagct tgccataagc tcaggaacat ttatcttgcc cacataagct
     1801 cttgcgtcaa tgccgtagtt ctccagaact cttgggtgaa gctcgccaaa aatgccgatc
     1861 tcctcaccat ttactttaac aacagcaact cttcccgggt ggaaagcata gaactcgtca
     1921 aagctacatt cagcagtagc cttttcaaat tcaactttct gtgcgccaag cttagagagc
     1981 agaccctcga caaaaccctt tatgtcatag aaatcagcct tcgtctcagt gtcatagaaa
     2041 ccaaaaccaa gtctttcagg ctcgttagga agcacctctc cctcaacagg tatatactcg
     2101 tttcctctct cgaagatata gcactcaggg ttgcggtagc tgtagtttct tgcaagcact
     2161 tcacatacag acggaatgat agtcgttctc ataacgctgg tgtcctcgcc aagagggttt
     2221 gtgataacaa ctgtctttct aagttttgag tcagcaggaa gatttatctt gtcaaaatac
     2281 ttaggggaaa tgaatgagaa tgtagatatc tcgttaagac ccatagcgag catagatctc
     2341 tgtatatgtc tgtcaaactt ctgcgcctcg gtgagcttag cctgtgcaac gcctctaacg
     2401 atagtgtttg gaatcttgtt gtagccgtaa agtctagcaa cttcctctgc cacgtcagcc
     2461 ttgcaggcaa tgtccactct gtaccaaggt gaaactacct ttccgtcctt tatctcaaag
     2521 ccaagtcttg tgagatattc caccatgtcg ctctcaggta tctcagtacc aaggaactta
     2581 tttatccact cagggtcaaa atcaaccgtc ttaggtgtct cgtcctcata gttctcatca
     2641 atgtaagtct ttacgacctc accagcacca agctcttcaa caagctggaa agctctcata
     2701 agagcctcat agcactcatg tgggtcaaga cccttttcat atctcgcaga agcgtcagtt
     2761 ctcatgccaa gcttcttggc tgttgttctt acgctcgcac cgtcaaagca ggcagactca
     2821 aatacaacag tagttgtatc gtccatgata ccgctgtatt cgccgcccat aacgcctgct
     2881 acagcaaccg gcttttcagc gtcagcaatg accagcatct cttctgaaag ctctcttact
     2941 tctccgtcaa gagttgtgat agtttctcct gccttagcgt ttctgatgtt gatagatgcg
     3001 tccttaacat atctgaggtc aaaagcgtgc atcggtctgc catattcaag cataacatag
     3061 tttgtgatat caacaaagtt gttgataggt ctaacgccgc agcctctgag tctttctctc
     3121 atccaacgag gtgacggacc tatctttaca ttttttacga taccggccat gtatctcttg
     3181 caaaggtcag tgttgtgtat ctttaccttg agcatatcgt tgatatcccc gtcaatgccc
     3241 ttgaactcag gcttcttgac tttcagctca gtgccgaaag tagccgcagt ttctcttgca
     3301 agaccgataa cagaaaggca gtcaggtctg tttgaagtta tctcaaactc aacagttgtg
     3361 tcattgtatc caatagcctc atgtatatcc ttgcctactg tcttgtcgca gtcatcgcca
     3421 agaatgaaaa ttccgtcctc tatagcatat gggaaatcgt gaacagaaag tccaagctcg
     3481 cccagagagc aaagcatacc attagaagca acacctctca gcttgccttt tgttatcttc
     3541 acctgcttgc cctcatgtag aacagatgac ttgtgctttg caactggcac aaaatcaccc
     3601 tcgtgaacgt tctgagcgcc tgtaactatc tgaacaggct cgtcctctcc aacatctacc
     3661 tgagtgatga acatatggtc agaatcaggg tgacgctcca tagaaactac cttgcccaca
     3721 acaacgttag aaatatcttc gccttcctgc tcaaagcact ctacctttga gcctgaaagc
     3781 gtcagagcgg aaacaaaatc ctttatcggc atatcgcagt ctacatagtc tgcaagccat
     3841 ttcattgaca aatccattta ttattcctcc gtaatcttat catttaccgc agttttgtat
     3901 cttctcgtcc aagtccttta aaaacagcgg tctataaata tagaaatctt tccaccgctt
     3961 tatgttcaca gtatatttta tcccaaaatt atcagttatc tcagctttca tgctgtcatt
     4021 gacaccattt gtgctgtgaa ctatggtctt gaactctcta atatccgtat attttataag
     4081 cagtttagcc ttaggcacat acatatatct gtcaagtaga taaaaggtgt tgtatttgaa
     4141 ctcgcttccc tctatctcgc tttccaaagc cagcatatcc tgagcgtcaa attgctccag
     4201 ccttgcaagg ttccctctgt attgttttac ctgcctttta aatcctctta ttccgataaa
     4261 aagcaggata cccagaaaaa tcaccagaaa cccacatagc acaagcatat cccatgcact
     4321 ctgctgttct tttacaagca tgaccgataa cccaccgcca acgcctacta tcagcgccat
     4381 aagtgtgctg atagccgccg acgttacaag cttagccttt gacggcacaa gctttctgta
     4441 aagcctgctc tggctcataa cttcattcat cagaactgct ctaaaaatct catatcgttc
     4501 tcataaagca gacgcatatc attgatgttg tatctgccca ttgttattct ttcaagaccg
     4561 ataccaaatg caaatccgct gtaaacctca gggtcaatgc cacagccctc aagtacctta
     4621 gggtgtacca tacctgcgcc gagaagctct acccagccct cctgcttgca cattgaacaa
     4681 cccttgccat ggcagttgaa gcacataagg tcaacttcag cagaaggctc tgtgtatgga
     4741 aaatggtgcg ggcgaagtct tatctgagtg tcctcgccat aaagacgctt cataagaagc
     4801 tccagcgtac ccttaaggtc agccatagtg atgcccttgt caataacaag accctctatc
     4861 tggtggaaca gcggtgaatg tgtagcgtcc actgtgtcag aacggtaaac tctgccaggt
     4921 gaaatgattc tgataggcgg ctttctcttt tccattgttc ttatctgaac agaagaagtc
     4981 tgtgttctaa gaacaacgtt ctcattgatg tagaatgtat cctgcgtatc tcttgcaggg
     5041 tgagatttag gcatattcag tgcctcgaag caataatggt ctgtttcaac ctcaggaccg
     5101 tccacaatat caaagcccat accaaggaac acttcctcta cctcttcaag agtagcgttc
     5161 agagggtgag gtctgcctat tcttgttttc ttgcccggaa gtgtaacgtc tatagcttcc
     5221 tctgtaagcc ttacctgctc cattttgctc ttaagctctt ccttacgctt attgagagca
     5281 ttctcgatat cagctcttac cttgtttgca agctgaccga taacaggtct ttcttctgcg
     5341 gaaagcttgc ccatttgctt aagtatggaa gttatctctc cctttttgcc aagaaatctt
     5401 actctgaaat cttccaacag cttggcgtca tcaatatgtg aaagttcttc tttcgcacgc
     5461 tcgtcaatgc ttttcaactg ttctttcatt acttatcctc ctataaaaat aagccctgcc
     5521 ccgactaaac aggacaagac tttgaaattc ttgtttatac cacctatcag taaagagcca
     5581 acactcttcc gtccttaacg cagacagacg ttcacgctta ctaaagacta tctctcattc
     5641 ggcatgacca ctcgcaagtg aacttcattt tgtcaggata taaagccgct ttcagaaaac
     5701 gcacacacgc cgcagcttct ctctgaagac ccttagcaaa actactctct cggtcatagt
     5761 gttttaagtg tattgataca ataatatgtt atcacattcc gccccacatt tcaagggctt
     5821 aacaaaaagt ttacaactac agcccatgct tatgaagcct gtcccacttc atatcctcct
     5881 cggagtaata atcgtcctcc tcgtcctcta tcttgttgta ttccttgtgt ggataaccta
     5941 tcttctcata aaagtcattg ataagcacaa gcttgccatt agtgtcctga aagctcagag
     6001 ctatcatacc gtcctcaggc acaaaagctt tctccctgcc ctctttgtcc tttaccacaa
     6061 agcttgactt ctccacattt ttcagcacgc aaacgagtct gcccttgttt gtctgataag
     6121 acttctctac ctcctcatag taaaggcata tgtgcagata gcttttagaa agtcccatgc
     6181 gctgagagtg ttcaatgtga tcctcatata tcctcgtgta aacatcagcg tcccgtgaaa
     6241 gcgagtaata ccagtacata agcccgccca cagaacaatt gcaagccccc agcagtataa
     6301 gcagaacctt tgcaaagacc gcgcttgcaa acaccgcacc aattatgatg ataacgaccc
     6361 caagcaggat attcttatac atattcctct gcattttcct gtgttcttgg atatcatgtg
     6421 ccgccttgac ctgcctctcc gcccttgaca gcttcggagg atattcataa accgcctgca
     6481 aaacattcac ctctcaactc tttgactttt ccatagcaag cacagatttt gtatagcgtt
     6541 tttgctttct aacagaatga aaaaagtctc ttattatcat ttgaactttc aaaatctttt
     6601 gatatcaacg ttcttaatgt gtctctgcac tcctcaatct gcctatacag aattgcgttc
     6661 tcagatctta gttcttcaat cttgtcaatc attttatggt ttaatgacat agattgtgtc
     6721 aacaaatcat acatttcgta atatgtttta ttcctcatta tttgtacctt cccttcacat
     6781 tatgcctgta ctatatgtgt ccactattaa taaagcagat acacagccca acaaggatta
     6841 ggatttacat tatgaccgtt tgggcaattc tgaatataca ccacataata agtgttgcca
     6901 tatttattgt acagatagtc caactcgcgc ttataaacct ctattgtgtc agaaagacta
     6961 ctttgcgtat atggtactgt atatactgca tcataacttg cagtttcggg agttacactt
     7021 ccttctttta attcaaaaaa ttcttcgagt gtattgaaat ctgttccata tttccaaaac
     7081 tctgtataat gagccttgat atactccgcc ttttctcttg aatacgctgt aagttctgcc
     7141 ttcatagcat c
//
LOCUS       DAWEMR010000134         7112 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4272_length_7112_cov_11.232677, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000134 DAWEMR010000000
VERSION     DAWEMR010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4272_length_7112_cov_11.232677"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2679..2688
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1995 a         1775 c         1381 g         1951 t
ORIGIN      
        1 atctatgaaa acgaacttgc ccttttcatt ttccacaacc gagtcagtag cgtcacgggt
       61 agtgcctgcg atatttgata cgataacacg ttcctcaccg catatcttgt ttattataga
      121 ggactttcca acgttaggct tgccgataac agcgaccttt atcgaatcat cgtcatcgtc
      181 ctcttcattt tcctccggaa gatatttcag cacctcgtca aggagatcgc ccgttccgtg
      241 accatgaaca gaagaaacag ggaacggttc gcccaaaccg agattataga actcatacag
      301 ctccataggc ggttcgccca cattatccac cttgttcacg caaagcacga taggcttgcc
      361 cgatttcaaa agcatctgtg ccacctcata atcagtggca gtaactccgc atttaaggtc
      421 tgtcacaaga atggtgacat cagctttcag tatagcaagc tccgcctgtc tgcgcatctg
      481 agcaaggata atatcatcag actcaggctc aattccgcca gtgtcaacca gcatgaactc
      541 gtgtccgagc cattcgcact ttgcatatat cctatctctt gtgacgccag gggtatcttc
      601 aactatagaa agtctttgac cgataagctt attaaaaaga gtagacttac caacattagg
      661 tctgcccact atcgcaacga tcggcaatga catatatatc gacctcattt catataaatt
      721 tgcacattga tacatcgtat agtataacac aatttctcac gttttgtcaa ccatacgaga
      781 aatacagcac ccacattcca tagatttttt actacaaata tgtagaagca aacagcgttt
      841 gcccgccttt ttcactgcta caacaaaaaa gagcagaacg gcctaagcca ttctgctcta
      901 tgtgcttatc aaaaattatg cttccttctt gataacctct acgcccttat aataaccgca
      961 attcttgcaa accttgtgtg gtgaagtaag ctcaccgcag ttagaacatt tacaaagtgc
     1021 aggtgcgtca agcttccaaa ctgctgaacg tctcttgtca cgtctagcct tggatacttt
     1081 tctctttggt actgccattc tggcacctcc tcgtcagtaa aatcattata gtctgttcag
     1141 ctatttcccg tcaagagcag ttacatttgc cctcattcaa atcctgtccg caaacgtaac
     1201 acaaaccctt gcagtcatcc ctgcaaagaa tttttgtagg cagctgtaac aacaaatctg
     1261 aaatagctaa ttcgtccaga tcaagagtat tgttctcgca gaccacataa tcgtccctgt
     1321 cgctttgaac acttctcacc agagtgtgtt caaagtcata aacatactcc ctgctgaact
     1381 cctttagaca tctgtcacag gtgtgatcca tagtaaaaac agtcttgaac ctgagcgtta
     1441 ccacccctgc cctgttctct atctcacctg ttaccacaac gggcgatgca aaagaatatc
     1501 cgccatactc cgacagcttg tctgctgtta tggaataatc gatcgttttt cgctctccga
     1561 ttatatcgaa aagttccttc aactgtacat ccatacttgc acctcaaaca tcacaaattc
     1621 cattataacc tgagaaacgt gatttgtcaa tacttttttt caattttttc gtttttgcta
     1681 tcaaaatacc aataaacgaa gcgcaaagcc ttacttgatg aaagccttta cctttgcagg
     1741 cagctcgtca acgtcagcag atactgtgaa agtaacgttt acgtcctttg tgagaactgc
     1801 taccttttca agcatattgc caagagcgtc ataatctctg ccgccgatct tcaggatacc
     1861 gtcaacgaaa acttcctgaa tgtcatagtt gccagccatc agacctgcaa caaagccata
     1921 gaagctgtca aagctgttga taccgtattc ttcagcgtca gccaatctta cattatgagg
     1981 aaggtcatag gtaagctgca tacctctttc gatgcaaaca acgttgcctg tagcattttc
     2041 agcagccttc ttgatagaat caacaagtat cttagtcttg cctgtgccct ttttacctgg
     2101 aatcaaatta atcatataaa ctctccgttc cgcttattaa agcatttatt ttaagagcag
     2161 acccccgaag gggaaacgct caaaatttcc ttttgttctg cctttaggat attagcccag
     2221 cttagcctcg agttcagcct tctgtgactc ataacccggc ttgccgagaa gagcgaacat
     2281 attcttcttg tagctctcaa cgcccggctg attgaatggg tttacaccaa gcatataacc
     2341 tgagatagca caagccttct caaagaagta gatcatctcg ccaaggtttt cctcatcgag
     2401 cttatcaaca tctataacga tattaggaac gccgccctca gtgtgagcaa ggatagtacc
     2461 ctgatatgcc ttttcattta caactgacat attctgattt gtgaggaaat taagtccgtc
     2521 aagattatcc ttgtcaggct ccaagaacag gtcgctcttt ggtgtcttga tgtgcataac
     2581 tgtctcaaac ataagttttg aaccgtcctg aatgaactga ccaagtgagt gcaggtctgt
     2641 agagaatgta gcggcagatg ggaatatacc cttatcgtnn nnnnnnnnca caactgcttg
     2701 aaccactcgc ccatcattgt gaatgaaggg tcatatgaaa cgagcatctc aacgctcttg
     2761 ccttttctgt agagaatgtt tctgagtgct gcatacttat agcaatcgtt gttatcatca
     2821 gcagaatact tcttcatacc ctctcttgcg ccagccatga gcttgtcgat atcacagcct
     2881 gctgctgcga tagggagaag accaactgct gtgagaactg aaaatcttcc gccaacgtca
     2941 tcaggaacca cgaatgtctc atagccctct tcgtcagaaa gctgcttaag agtaccctta
     3001 gccttgtctg ttgtagcgaa aattcttgtc ttagcctctt ccttgccata cttatcttca
     3061 agaagcttct tgaaaattct gaaagcaagt gcaggctcag ttgttgtgcc cgactttgaa
     3121 attacgttta cgcagatatc tctgccctcg caaattgaaa gcacctcgtt gaggtaagcc
     3181 gagctgatat tatttccgac aaaatagatg tcaggagtat ccttcttaag attgttgtag
     3241 aatggcgact tgagcagctc gatagcagct cttgcaccaa gataagaacc gccgataccg
     3301 ataacgatga gaacatcgca cttttccttg atccttgcgg cagcagcctt gatccttgca
     3361 aattcttcct tatcataatc ctcagggagt gttacccagc cgagaaaatc atttcccaga
     3421 ccgtttctgt tcaccagagt gttatgagct gcagttacct gcgcagccat accctcgtat
     3481 tcatgttcac ttacaaaacc ctcaagatac tttgtgttaa gctttaatga cattatcctt
     3541 catttccttt ccttacctgt cggagagcgt gataccataa aacacgttcc tccgctaata
     3601 ctagataaat tatactatat ttttgtgaac atttcaagag ttttataaag cgaaatcatc
     3661 ttttgtacat tttaaacaaa tataatatta catactcagc aaattgtaca caagcttttt
     3721 tatgccgcag ataaaattca ttttcttcac ctctttgcag gcgactatct ttccctctaa
     3781 tatcacctca ccgcccatgg tgtatcttat ttctcccgca gtttcgccat ttttcacagg
     3841 tgcgtcaagc ttctcctctg ccgtaaaatc gcactccacc atacgatatg ttcccctcgg
     3901 tataactata ggataaagcc cctcgggagc aagctccaca gaaagctcct ctcctccgca
     3961 caccacagtt tcctttacca tatcctcagg aagttcaggg gtgtatatct catatgtatc
     4021 gaacgccagg ttcagaagct tcttcgcctc agcattcata tcctcctgcg aatcacagcc
     4081 gttaagcacc acgcatactg acatatcccc tttttcagcc gttgccgcaa tacagcttcc
     4141 tgccttttca cttccccacg ctttcatgcc tgttacgccc ttataagtac gcacaagcct
     4201 gttaagattt acaagctccg ccttgccgcc cctcacagtg tcaagccagc aggtaaggta
     4261 aggagtgaga tttttgtatt ttgcaagctc cgaacaaagc aaagccgtgt cctttgctgt
     4321 tgtaacattg ccctctccca ttccagtaca atcagtgtaa acagtaccat tcatgccaag
     4381 cattttcgca cggctgttca taagctccac cgccttgtct gccgagcctg ccaccttttc
     4441 cgcaagtgcc gcacagccgt cgtttgcatt gcctgccgac actgatttta taagctcctc
     4501 cacagatatc ttctctccct tgtcaagcca tatctggctt ccctgctgag agttggcata
     4561 ctccgacacc actgacgtgt cgctcatgga aagctcaccg ctgtctatct tttctgctgt
     4621 gagcaaaagc accatgagct tcgcatagtg accgcactca cactgctcct gcggctcttt
     4681 ttcaaaaagc accctgcctg tggtcatctc cgtcaccact gcagacctag ccgtaagccc
     4741 ctctatcata tcgctcaccg ccacagtgtc cgcaggcaca aagttctccg ctgacagcgc
     4801 acctgctttt tctttcccct ccgccagcga acacaccata aacactgcca ccgctgcaca
     4861 taacactgcc gagcatattt tccttaatat tttcacattt tccacctctc acatatacta
     4921 tttttatatt tatattcacc ctgtcccgaa attagcatta tgccacgaaa agtttccgga
     4981 aactcatttg taaatagttg caggaatttg ttaaataact gttacaactc ttgaaataaa
     5041 tatgggagtg tgctataatg tattctatag aaatgtatat tttataagcg aaatatttat
     5101 atgaggtgat ttaacaatga cagaaatgga actttacaag ctttggtgtg aaaaggctgt
     5161 tgacgatcct gatctccaaa cagagcttaa gtctatcgag ggcgacgagg ctgccatcca
     5221 ggacagattt tacagagatc ttgaatttgg cacaggcgga ctcagaggag ttatcggtgc
     5281 aggcgcatac agacttaatg tttacactat cagacgtgca acacagggtc ttgcagacta
     5341 tgtaaacggc gcatttgaaa acggcagcgt agctatcgca tatgacagcc gtatcaagtc
     5401 tgacaagttc gcaaaggaag ctgctgcagt tctggctgca aacggcataa aggtatacat
     5461 ctattcagag ctcatgccaa ctccaatgct ttcatgggct gtaagagagc ttaaatgtca
     5521 ggcaggtatt gttataactg catcacacaa tcctgcaaag tacaatggtt acaaggttta
     5581 cggcgaggac ggctgtcaga tcacactcga tgttgcaaac acagttatcg gcaagatcaa
     5641 cgcagttgag atgttcggcg gtgcaaaggt aatcgacttt gaggacggta tcaagtcagg
     5701 caagatcgaa tatatcaagc aggacgttat cgacaagtat cttgacaagg tagcacagca
     5761 gggtgtacat actgacctcg tagcagacag cggtctgaag gtagtataca ctcctcttaa
     5821 cggcacagga aacaagcctg tgagagctat ccttaagaag ataggcataa aggaagtaac
     5881 agtagtacct gagcaggaaa atcctgacgg aaacttccct acctgcccat tccctaaccc
     5941 tgagataaag gaagcccttg caaagggact tgagctttgc aagacagtaa agcctgatct
     6001 tcttcttgca acagacccag actgtgacag agttggtatc gcggtacctg acccagaggg
     6061 caactatgtt ctgttcagcg gaaatgaagt aggcgcaatg cttctcaaat atatttgtca
     6121 ggaaagaaca gcccttggca caatgccaaa ggaccctgta gctgttaaga ctatcgtaac
     6181 aacagacata tgcaagaaga tagcagcaga gtacaatgta gagcttagag aagttctcac
     6241 aggcttcaag tttatcggcg agcagatcgg tttccttgag aaagagggac aggacgacag
     6301 atatatcttc ggatttgaag agagctatgg ttatcttgca ggctcatatg taagagataa
     6361 ggacgcagta gtaggctcaa tgctcatttg tgaaatggca gcattctaca gaacaaaggg
     6421 catctcactg cttcaggcaa gagaagatat gtacaagaag tacggaaact atctccacag
     6481 ccagaagtca ttccagtgcg agggtgcaag cggaatggag agaatgaagg agatcatgac
     6541 agatctgaga gctaatccac ctaaggctat tggcggactc aaggtagtag acatagcaga
     6601 ttatcttgca tctgaggaaa caaaccttga aacaggcgca aagacagttc ttacacttcc
     6661 aaagtcagat gttatcgcat tcaagcttga gcagggtgca agtgtaataa tcagaccttc
     6721 cggcacagag ccaaagatca aggcatacta cacaactatc ggcgacacaa gagctgatgc
     6781 agaagcacag gaagaaaagc ttattgaaga tttcaagggc attcttggtt tctaataatt
     6841 actgaatata gcgaaagcag gcgagtcagt tttttgactt gcctgctttt ttatttgcga
     6901 tattcacaca atgctttgcg tttcacaaac catgtagggg agcagcgcaa tttctgcaag
     6961 cagaaatgcg acgtcccgct tctcgtttgc ctgtccaatt ctacgttatc cgcacaacgt
     7021 tcggcatatt ttttcaaaac gctgtacggc acaacatttc cgcacacggc acatctacac
     7081 aaattcatga ataatcaaac cttaccgctt cg
//
LOCUS       DAWEMR010000135         7063 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4313_length_7063_cov_17.055651, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000135 DAWEMR010000000
VERSION     DAWEMR010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4313_length_7063_cov_17.055651"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1988 a         1630 c         1747 g         1698 t
ORIGIN      
        1 gcaccgcaca ttttatccgc accgaccttt tgactgcggg cgttctctcc aatctgcgga
       61 aagtgacgag ctatgcggcg aagcacgaaa agcagtttat caagctgctg atcgagcaga
      121 acgaggatgg cggcaagcgc cggaacgccg ccaaacgcaa ggaactggac gcagccgaaa
      181 agcgaatttc ggagttgtcc gccattttca agcggctgta cgaggacagc gtaagcgggc
      241 gtatctctga cgagcgtttc acagagcttt cggcagacta tgaagccgag cagcaggaac
      301 tgaaagagcg tgccgccaga atacaggcag agctttccaa agcacaggcc gccacggtca
      361 atgcagaaaa gtttatgaat atagtccgca agcacatgaa ctttgaagaa ttgacccata
      421 cccttttgcg tgagtttgta gagaagatcg tagtgcatga gtgcagctat gacgagaacg
      481 gcacacgcag acaggacatt gagatttatt actctttcgt tggcaaggtg gacttgcccg
      541 aatgaccgcc cgacctatcc gacacaaagc gcaagtgccg gataggaacg gcaaaatttt
      601 ttacacttct attacttctt tatcacatat cagtaaagtc tcagggcatg aagcagctta
      661 tggctggtct tggtctgatc ctcctcgcta tcattctcgt acctgttctt gagggtatga
      721 tgacgggtgc ggtgtaatct gacacacgct aatcaagaaa agagacaagc gtaaacctcg
      781 gctgaacgct atataaatga tgccgttcca atagaattat gaaaggtcgc tcagcggtgg
      841 gctgtggtgg gtatgggcga taagacagaa tgggaataaa catcggttat ctgacagcga
      901 accgaacaag tgcaggagat gaggtctaca caccatttta tgcggtagaa ccgttactgg
      961 agtttcttcc gaaagacaag aagatatggt gtccgttcga tgaggaatgg agcgctttct
     1021 atcagttcct ttcggagaaa ggctatgaag tggagcgaag cagcttaaag gagggacagg
     1081 actttttcag gtatgaaccc gaacattggg acattcttgt gtccaatccc ccattcagca
     1141 aaaagaatga tgtactgaaa cgagcatttt ctttccaaaa gcccttcgct ctgctcctgc
     1201 ccgtgaacag tattcaaggc aaggcgagat ataagatttt caacaacgaa atccagatgc
     1261 tctcctttga cgggcgtgtg gactatcata cacggcagaa catggaatgt accactaagg
     1321 gcaatcactt cggctctgct tatttctgcc gtgaccttct gccctccaaa ctggagcttc
     1381 ggcagcttgt caaatatgac agacctttag tcactcccac gattggaggt gatgaataat
     1441 gggaattatc agcgatgcga tcgatgcact ggaagattgg tgtaaagact tatttaagga
     1501 cggcatccag tctcagtttg acggcatttc cgacctactg accgacactt tctcgcaaac
     1561 aacgggaaca ggcggcagcg gtggacttgt caatgacttc gtaactcagc acccgtcaca
     1621 gttcaccggc ggaggtactg gcggtactac gatctggaca acgatcgaaa cgctttgcaa
     1681 caatgtcgta gtgcctatcg gcggtttcat tctgacagtt atcctgctga atgaactgat
     1741 acagatggta ctgcgaggaa ataacttcaa ggacttcgat gattctatct tcattaaatg
     1801 gatcatcaaa gccttgtgcg gtgtaatact ggtcgccaac acctattaca tcgcttcggc
     1861 actattctcg tttggcacaa cggtatgctc caatgggctt gccacactat tcgggaccgg
     1921 agattatcta tcttccacac tctcgatcaa tccgtcggct ctcagcggtc tgagcttggg
     1981 tgagctgatg acggtgtggt ttatttccct gatcgttcat cttggggtgc tgatactgat
     2041 agttgctatc gttatcacac ttgcaagccg tatcatcgag gtgtttatgt atctcagcat
     2101 tgcacctatc cctatggcaa ccatgatgga cagcggtgaa tgggcttcta tcggtaagaa
     2161 ctggatcaaa cagcttcttg ctctttcttt ccagggcttc tttatcgtgg tggcactcgg
     2221 tattttcaag acgctgttca gcaatgcgat cgttgccctg aatgcaagta cagacggcgt
     2281 gatcctgcaa atgggactgc ttcttggcta tactgcggca cttatcttca cgattctccg
     2341 tacaggagct atcagcaaat cagtctttaa cgcacgataa aacaagatcg gaggtcaaaa
     2401 atggcacatt acgttcctat cccgaaggac ttgaacgaca tcaaggaaaa gttcatcatg
     2461 ggatttacaa aacgtcaggt gatctgcttc ggcatcggtc ttgttttggg tgcgcccgtg
     2521 tattttctga caagagcgtc tatcggaatg tcgggagcga tcttcgcaat gggggcggtc
     2581 gccgcccctg cgatcctctg cggcatttac aagaaaaacg gcgtgttctt ggagaaacag
     2641 gtgaagttca tgcgtgaata cttcacaaga ccacgaaagc ggtattaccg cacaaccaac
     2701 gtattcatgt gcattgaacg gcacatcgaa tacaacagaa tcaagaagaa gctggcagat
     2761 gccgaaagaa aaggctaaca gctttaccta tacataatag tataactttc ggtatctccg
     2821 cttttgcata taacggaggt ttccaatggg ctttagaaag aaaaatgcca aaagaagtga
     2881 taaccgcagt tctgctaagg ttatcatggg caagcctgcc tctaaactga caaaagagga
     2941 aaaaaagatt ttatccgcac gaatggcgga gatcaggagt gaaaacggcg gtaaatcgac
     3001 tgtgcagaac accattccgt acctgtgtat gtatcaggac ggcgtgtgtc aggtgtcgga
     3061 gaatttcttt tctctgacgg tgcagtttta cgatgccaac tactctatcg ctgaatttga
     3121 ggagcagaac aacattttca gcaaatactg tgacattatc aatcttttcg ataacacgat
     3181 caaattccag cttacctttg agaaccagaa ccgttcaaag gcaaagctcg tcaagacggt
     3241 gcagataccc gaacaggacg acgatttcaa tgcgatccgc aaggagtaca gcgaaatgct
     3301 caccgataag ctactgaaag gcagtaacgg tcagagtgca aggaagttcc tgacattcgg
     3361 tatcgaatcc acatcataca aggcagcaag ggcaaagctg atgagtatca agaatgacgt
     3421 gatcaagggc ttcaaagcct ttggcgttga tgccgaggta ctggacggca gacagagact
     3481 tgaagctctg tattatgccc tgaaccctta cagaaacgcc ccattcattt tcgattggga
     3541 ctctatgctg agggcaggaa tggacacaaa ggattttatc tgtccgccct cgatcaaatt
     3601 caataaggcg gactttgaga tcggcaatgc ctacggcgca gtatggggta tgaatatcct
     3661 cgccggagag ctgcccgatg agatactcaa agacttcctt gaaatgcaga accttttctg
     3721 cgtgaacatt catgttgatc cgctggacca gatagatgcc ctgaaattcg tcaaaaagaa
     3781 gctcacgaat gtggagcaga tgaagatcga cgaacagaag aaagcatcgc agtcgggcta
     3841 tgatccggac attctgcctc ctgcgatcaa gatgtatatc gaggacatcg aaaagctcct
     3901 cggtgacttg aacagcaaaa atgagcgatt attccatatc agcatttcac tgagggcata
     3961 cgcaaaatcg aagaaagacc tgaaacttct ttctgagatg ctgaaaagaa cttgccagaa
     4021 aaacaactgc atcatgttcc cgtatgatta caggcaggag cagacacttg tatccacgct
     4081 gcctatcggc tataacgaga ttcccgtccg tcgtgagatg cacacttcgg gcattgcgat
     4141 tttcgtaccg tttaccacaa aagagctgtt tcaggacgga caggctacct attacggtat
     4201 caatacgctg tcgggcaaca tgataagagc aaatcgctcc cgattaaaga acccgaacgg
     4261 tcttatcctc ggtacgccgg gtgccggcaa gagtttcagc gtcaagcgtg aaatcctcga
     4321 ctgctttctc actacgctcg atgatattat catctgcgat cccgaaggcg agtattttcc
     4381 gcttgtatcg gcgcttcacg gtcagcttat ccgcatcgcc agcaattccg aacagcatat
     4441 caatccgatg gacataacca ttgatgacta tatgttcacc aatcctatgg aagtgattgc
     4501 aaacaagagt gattttctta tcagcttgtg cgagatcatt gtcggcggca gatacgggct
     4561 ttccgctgag gaacgctcgg tcattgataa gtgcgtacag cgtatctata agcatttcat
     4621 cgagaatgaa ccgactgagg acaagatgcc gcttctctcc gacttacagc gagagcttca
     4681 tgaggagggt gaagtcgctc tgcgtgtggc gaacagcctt gaaatgtttg taaatggttc
     4741 tcagaacctt ttcaatcacc gcacaaacat tgatatgagc aatcgtatca tctgctttga
     4801 tattaaggaa cttggcaacc agctcaaaaa ggtcggtatg ctgatcgttc aggatacggt
     4861 ttggaacaga gtttccgcta accgtgagaa aaagaagatc acccgctact acatcgatga
     4921 attccacttg cttctgaaag aggagcagac tgcgaaatat tccgctgaga tatggaagcg
     4981 tttccgtaaa tggggcggag ttccgacagg tatcacgcag aatgtcaagg acttgctttc
     5041 ttctcaggag gtcgagaata tctttgataa ctcggacttc gtgtatatgc tcaatcaggc
     5101 agccggtgac agggacattc ttgccgagaa actgcacatt tccaaagagc agatgaagta
     5161 tgtcacgaac agcggtccgg gcgaaggact tatccgttat gataaggtgt tactgccgtt
     5221 tacggataaa tttccgcagg atacgcagat gtatcgcctt atgacaaccc gtcccgctga
     5281 ccttgcataa actgcgcaga atggaggatt tatgagctta caacgaaaag aaaataacct
     5341 ccgcagagcg caggcgcgtt tcgataaagt cagcagacgg tcgaagcagt ttcagataga
     5401 cttccgccgt gaacagaaca gcagaggtcg ctttcatacc cgtgcccata tccttcgtgg
     5461 tgagaaaacg caccacggcg gcaggggtat cattcacaag tttgcaaatg caaaataccg
     5521 tattacgggt gatgctccct cggtcacaag gacgatcaaa tcatggcagc ctaagacctt
     5581 taagggcaag ctgttcaagg cgaatgcccg tgcggtcaat tttgcagtac atgatgtagc
     5641 gcagacggcg gtagatacgg ctcttgcgac agaaacggta gggctgaaag tatcggatac
     5701 tgcccagcgt gaagtccgca gcaaactcaa acagaaatat acccgtgaag cggttgacga
     5761 ttatcaccgt ggagtatttc tcatgggacg gacggcgact gatgctgtca agggcgttca
     5821 cagtcacctg aaaaccaaga agcagtacaa gctcgaaaag gcaaagttac agctcaaaaa
     5881 ggcggataac aagctgtatc gggcaaaaac gaaccgtccg aagctctgtg ccaattccgc
     5941 cgatctgcgg aaagcgaaag cggagtatat cggatttctc cgtgagttta tgggaatgtc
     6001 ctttgtagaa gccgttgaag ccctcaacgg tgaacactat gacctgacat atactccgtc
     6061 ccgcacctac gaacagaagc cgaaaacagt aacggcaagg gagctttccc ttgccgaagc
     6121 tgataattgc aggcgtgtat ttgcgtatct ctgcaagacc agagggcttg actatgacct
     6181 tgtggcttca cttgtgagac agggcgtagt atctcaggaa gaaaagacag gcaatgtgct
     6241 tttcaaatat tatgacgata acggcaaggt catcggtgct gaaaaggtcg gtacttcgac
     6301 agaacaccga ttcaagggta ttgccgaaca ttcagccgat gggcacggct ttgaggttgt
     6361 ccgcggcacg ggcgaaaagg cttttttctt tgagagtgca atagatatgc tctcctatct
     6421 gcaaatgcac gataaggaaa tgacggattg caggcttgtt tccatgatgg gcgtgaagcc
     6481 gaatatcgtt cttgacacga tgctccgtca taatatcccg cctgagaatg ttttcctttg
     6541 ctccgacaat gataccgcag gcaatgaatt tgcccagcgt ttgcaggagc agtatccaga
     6601 catgaagcgt gttatcactc ccgatacata taaagactgg aatgatatgc ttcgttgtat
     6661 cccaaaacaa gtagaacatg agactgaaaa aaaggaggtt cagcaaacga atatgcagta
     6721 ttacggcaac gaaatgtgga acaaggcaac agataacaga gacaaaaacc ttgtgacgat
     6781 ccaggctgcg gattttgcaa gattgcagaa acagcttgat aattcgggca tcaattacta
     6841 tgcctatgcc cgtgataatt ccgtcattat ggcgatcaat gataaagata tagagtggtt
     6901 caagcgtatc gcaggcactc ccgaccttgt tcccaccaaa tccaacagac cttattctcc
     6961 gcccgaaaaa aacatcttcg gttcagcgga gtatcgttat attccgcaga aagagtacct
     7021 ttccgctgac cgtgaccttg tgctgaaaat ggctgagatc atg
//
LOCUS       DAWEMR010000136         7027 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4347_length_7027_cov_11.976764, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000136 DAWEMR010000000
VERSION     DAWEMR010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4347_length_7027_cov_11.976764"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    361..370
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2114 a         1498 c         1636 g         1769 t
ORIGIN      
        1 ggtatagtaa caaattatac ttttgagagc acaatgtttt tggcagtgtt cattataatg
       61 agatcattta ctgggggata tcatgcaaat acatatatta aatgtaattt agtgacctca
      121 atatcttttg ttatattgtt gttgatattt aaaataataa gacatatttc tttaaagtca
      181 attatattaa ttgctatttt tcaagtagta accattgttt tattggcacc gattgagaac
      241 aagaataaac cgatagaaaa tagaactgtc tataaaatta tttctaccgt tttatcagta
      301 gtgctatctg caataagcat cgtattaatt ataaaaggtg tagacgtagg gctaataatt
      361 nnnnnnnnnn tttcaatatt tataatatac gcaagattta tgaaaagggg ggatttctta
      421 tgaaaattaa agttgctgta gcaaaatcag tcaaggcgct tgttggacat atggcaaagg
      481 cagcctgtgg ttctgcatca cattggggag catatcaacc taaagaacct aaatttaaga
      541 aagaaaagaa gtaatagata accacagaat cgtttaataa tcggtttaat taggaggggt
      601 aaggtgtttc caatgcagag ataaaataca caaaaatgta caataacatt tgtgcgaaca
      661 tacaaagttt aaaatgattt gcaacaaagt agatcgtttg tgatactaat atattaagac
      721 aatggaggta tttaaaatga caaaaatggt taagaagata gcagctatgg gtgctgccgt
      781 aatgatgatg gcttcgattt cggctatggg tgcgagcgca tatactgatc tcgaaaaaaa
      841 cggagcgtat tgcactgctt acaagagttc ctcaagcagc gtctatgctt caacatcaac
      901 aaaggacgga tcgaaaaaaa cggtaggagt taacatcgtg ctttactata caaagagcgg
      961 aaatacactt aacacaggta gcggcaacag ctatacaaga ggcgttagca caacctgctc
     1021 cacaccttcc ggcggaacgt ttaaaagtgc gacaataaca cataaattaa acaacgctac
     1081 tgtatataca ggttacttgg tataacggat aattctaaaa aataatgccg cactgacata
     1141 tagtgatatt tccgtgcggt attattctat agaatatgga gataaccgaa atgaaaatta
     1201 aagcattgat cgtaatgacc atgaccgccg ctgccctgct tctgccatca tgcggaaaac
     1261 agccggataa gacctcggac gcaaatgtaa acgaacccgt cgaattcggg gcgaatataa
     1321 ccgtttccga cagctacgct ttcagggaaa acgacggcag actttacctc tgggatttga
     1381 aaaccaacag ctatgagatt ttctgcacac agcccgattg taagcatcag accgctgcag
     1441 aaaacgccga tacaaaatgc tcggctgtcg ctccaaaaga ggggtacttt tataactatg
     1501 cttttcttta taacgatacg ctatactcga tatgcacggg cgagctgaac gagtttctta
     1561 tttataccgc cgataccgac ggcatgaaca gaaagcttgc gtttaccgcg gaactgtcgc
     1621 tttgcaccat ggttaatcct gtgcttgacg gcggcagact gttctttgtc ggctcggaat
     1681 ttgaaatgaa cgataccgac agtgttggat tgcgggagaa ttattcgctc tgttcggttg
     1741 acctttccga tttttctttt gaaaaatata tggacatcgg cactgcaaac gaaaccgtga
     1801 tcggtaagaa gagtcttttg ctgtacaaaa acaagatata tttccagcac agcgaatatt
     1861 ccgaaaacag cgttcattcc gctgtcgaat gctatgatat cgacggctcg gagaggatga
     1921 cggtacttga aatggaagat tccgtcaacg tctggcagtg caacggcggt aaaatgcttt
     1981 atgtcgtttc cgacgatgac gattcccatt cgcaggtctg ctcctatgat ctcgacggcg
     2041 gtgggacaga ggttttgttt aaacgggacg gctatgtcag cgatgtctgc atgatcggaa
     2101 acagggtgtt ttatatgtac agtactgcgg acggcaaaag cgagatacgc agtgcaggag
     2161 tgttcgatac gtcggacgga aaagacctta cgaccgaatt tgacggcgat gtttacgtat
     2221 cggttctggg acacacgtca aacgggcatt tgatacacta tcaggactcc gaacgcgaca
     2281 gcttcggact gctctccgac gaggactttt ggagtctgaa atttgaaaat gccgatttta
     2341 aatttgagca gtaagagggg aatgagcaat gggtaagaaa gaatatttag cctgcctgat
     2401 ctcgctggtc atgctcggtc taacagcgtg ctccgataat gcaaacaaga ccggctcgga
     2461 agtaagctca ggtacttcat cagccgaaga aaaggattac ggttacggct ttgacaagtt
     2521 cggattttcg cgttttgacg ttgaaacggc agaatccctc acttatgaaa ataaaacaaa
     2581 gatcgtatat cttacaaact acgatacgac ctttacaact actgacgacg acccgagaat
     2641 atacgatgaa atttacattg aaaacactgt gaacgagtat ctggataaaa acggatatga
     2701 cttttacgtt gattttgtaa tgaacggcga gtttgatttc aaaacgggcg agctgtaccc
     2761 aaacatggac gtttacgaaa gtatgctcaa aaataacgag caggtcgata tagtcaacac
     2821 ggggctgggc atgagcgcgc tgggcggtta cggagatacc tttaatctgt ttgtagaaag
     2881 gggttatcta gaacctttga acgattattt tcagaccgac agcggaaaag cgttttacga
     2941 cgggttcgac ggcatagtct ggaagcagac gacctgtgcc gacggtatga tatacggaaa
     3001 aaccaccgac tatacgcttg ctcagccgct ggtattgttg cttaacggcg atgtttgcaa
     3061 cgaatacggg gtaaatacag acgatatcca aagcctagaa gatctcgagc cgtatctgca
     3121 aagccttgcg gacgagggga aacgtggtct gctgctcgac agcacctgcg aactgtatta
     3181 tgagatggcg ggattttgca gatacaaggg cgtgtatatc aacgccgaaa cgggactggc
     3241 ggaaaatatt ttcgagaatg aaaaagcctt gaaatatctc aagaccgtat atgaatattc
     3301 gcaaaatgga tatatatcaa acaacgtcga tattgcgaac gacgcttata tttgcagcct
     3361 gtcgccggca atgccgcttt attatgattc gtcgaaaatc gtttcaagcg gatatttgca
     3421 gcaggaggaa ctgaacggag tcgtggggat atcgtcctct agcaagaaca aggaaacggc
     3481 gtttgaactg ctcgctctgc tgaacacgga cgaagagctt gcgaacataa tttacaacgg
     3541 cgcagaggga cgaaattatg cggtcaagga cggcgaaaag tacccgaaca agaacgctct
     3601 gccgttttac gacgtcgccg cgacaatgac taattccatt atcgccgaaa gcaattcgca
     3661 ggacaatcaa agcaagcgga aggatattgc gatatgctgg gagcattccg aggtatctcc
     3721 gttttacggt ttggaggtat ccgacgatct tgccgaaaaa ttggagaaaa cggcggcggt
     3781 ctatgacgat ttttacggtc tgttctacgg cgattacggc gaatatcaaa gccttgacga
     3841 agccttgttc gcagcaaatg aacagctgaa agccgcagga atagacgagg ttttaaacga
     3901 gctgaacgag cagcatggaa aatttgataa agaataaaac cggagaaaac aacatgactc
     3961 tcaccctaac aaacctcacc aaacaatacg gcaatttcac cgcacttgaa aacttcacct
     4021 acgacttcac ggcgggagta tacggtctgc ttggaccgaa cggtgcaggc aaatccaccc
     4081 ttatgaatat catcaccgac aatctgaaac caacttccgg ctcggtgaaa tttgacggcg
     4141 ttgaaacaag cacaatggac gccgacttcc gcaaaattct cggctttatg ccccaacaac
     4201 aagggattta tccaaacttt acgctggagc gttttctcta ctatatggct gccctcaaag
     4261 gcatgaaaaa agctgacgca aagccgcaga tagaaaagct aatcaacctt gtcaacttgc
     4321 aggacagccg aaacaaacgc ctcggcggct tttcgggcgg tatgaaacag cgtgccctca
     4381 tcgcacaggc tatgctcggc gaaccgaaag tcattatcct cgacgagcct accgcaggtc
     4441 ttgacccgaa ggagcgtatc cgtataagaa atctggtggc acaaatagct ttcgacaaga
     4501 tagtcataat cgcaacccac gttgtttcgg atatcgagtt tatcgcaaaa gaagtcctgc
     4561 tgatgaaaag gggcgagata atcgacagcg gtgcgcctca tatcctctgc gaaaagatcg
     4621 acggcaaagt gttcgagata accgccgacg agagtatgct ggacgaaatt tccgcaaaat
     4681 acaaggtggg aaatatctca aaggacgaga aaaatatcta cgtccgtgtt atcagcgaac
     4741 agccgccaac ttacgaatgc aaaacctgca agcccgacct tgaggacctc tacctctact
     4801 atttcgattg agggggcgag ctaatgatct tcgtaaacga actgataaaa gcgttctgca
     4861 aacgcacaac tattgcaatt ttcgccgttc tgctgctcct caacggcgtt ttgctgtata
     4921 taaacgaaac aaaacagacg ctggaataca cccctgagca gtacaaagcc gcttatcaga
     4981 ccctcgaagg gcttgacact cacgttgctt ttgagcgaat ttcacagaaa aagtcggagc
     5041 ttgaattgat acaaaggctg tcattcggtg aggatatttc gcaggaaaac tgcaatgctg
     5101 aggagctttt gaagagctac aagacgaaga gctacctaga atttaccgac gatatttact
     5161 ccgagataga gctgaccgac cgcatttacg aggaggtcgc cgcctgcgaa aattacgaca
     5221 gctaccttga aaatatcgac agcaccgcgc gaaaaatgac gggcatttcg cttttcgccg
     5281 accccgatag tttcagctac aaaaatatcg cacaaacgcc tgcggacttc gcctacctca
     5341 aaggctcaaa gctcaccgcc gcaccctcaa agggtatttc aatggcaaca ggtttcctcg
     5401 ccaccgacct tatcgcaatg ctgatgataa tgaccgtggt gatgaccata gtcacccgtg
     5461 aaaaagagct tgaccagata acactttcaa gaacaacata caaaggcaga atgccccttg
     5521 gtatcacaaa gattttcacc tgttttgcgg cggcgattgt ggcggaaatg ttgctttacg
     5581 gagtaaattt tgcagtttca tatattacat atggtttcgg cgacctgtca aggcagatac
     5641 agtcggtata cgagtttaac ggcagtaact tgaaaatatc cgtactgcaa tactttgcac
     5701 ttttccttgc ggcaaaactt gcggtttact gcgtatttgc ggcgatgatc taccttgtta
     5761 ctgtggtatc aaacaccgct gtaaaggtct acggcatact gataattacc atagcggcgg
     5821 aagccgtgct ctactacacc ataccgagca caagctatct ttgtccgcta aaatacatta
     5881 atattcttgc atacgccaac acaaaagacc tcttcgcgag ctatctgaac ctcaatattt
     5941 ttggaaaacc tgtgaactat atggctgtgt ttgtcggctc ggcgatagta ttgctgctca
     6001 tattgtccat actctccgtg ctgatttttt caaagcaaag ggttattaaa agccgtacaa
     6061 gaaaattcag ccttgcaaag ttcagtattt tcaaaggcag gacaacaaat ctctttttgc
     6121 aagagtgcta caaggtcttt atcggcggca aggctctgct gattttgata gcatttgctg
     6181 tcatcaccgc agtttcatat tctcctatca gcgagagctt ttcatcagcg gacgaggttt
     6241 attacaagca gtatatgttg aaatttgagg gcgagtacac cgccgaaaag cagaaaatga
     6301 tagacgctga ggcgcagaaa tttgccgacg cgcagatgaa aatgtcggag gaaatggcaa
     6361 attcagatgg tgacggtgtt ttcataatga tgaagtatca ggatatactt gctccgcagt
     6421 acgcctttga cgaggtgaaa cagcacgccg aatatttgca gacaacagag gatgggcagt
     6481 tcgtttatga cagcggctac aagctgctga caggcggcga aaccgcagga aacaaggact
     6541 taacgctggg tctgacggca atgacaatgg tgatactttg ccttacctat gtctatgccg
     6601 ccgagtatca gacaggtgcg aacgttctgc tgaaaacctc cgcaagaggc agagaggaca
     6661 catttctctg caagttcggc ataggcgttg tcattgtgac gatgattttc gcactgacct
     6721 atgcgccgta cttttacaac gtgctgaacg catacggcac aagggggata aacgcacctg
     6781 cctgctcaat agagtcgctt tcaaattttg atgtttccat aaaaggttat ctcattctca
     6841 taatcgccgg aagatacctt gcgctgcttt gtgcaatgct catcatctat tttctgtcag
     6901 cgaagctcaa aagcgttata tcgacattcc ttgcggcgac ggcggtgctt attttgccga
     6961 tattgcttgc gctgctgggg atagggttct ttgattatgt gctgttgaat ccgattctaa
     7021 tcgggaa
//
LOCUS       DAWEMR010000137         6974 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4381_length_6974_cov_7.351496, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000137 DAWEMR010000000
VERSION     DAWEMR010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4381_length_6974_cov_7.351496"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    316..325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3525..3534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1864 a         1499 c         1342 g         2249 t
ORIGIN      
        1 ggcgaatatc ctcagtccgt cacagcatat ataagcatat tccgccataa cagggaagtc
       61 aagcaccatt tgatcgactt ctgtgtcaca attaccacgc acgcatagaa gcttatcctt
      121 tagcggattg agcatggcta tcacctgctt tggtgcatat tcctttggaa ggtcgtttct
      181 tggtccgtga tagaggatat cacccaaaag aattagtttt tccgcgccgt tttgctcaaa
      241 ggcttcaagc atttttttgc accagtaagc cgaaccatga atgtctgacg ctatcataag
      301 tttcatattt tacacnnnnn nnnnnatgat ctcagatgca agcttgtgca aagacacata
      361 ctctggcttt attttgttat atatatcgca gcctttttcc tttttgaact cttttaccat
      421 tctgatataa agctgctgtt ttgttgtggc atcaatgact gcttctctta taagcgccag
      481 ttcgtcttta ttgcatttgc cgtcgagtag ctcatcaagt tttacggata gtacatcatg
      541 gtaaacaacc ttttcagttt tctgagccgt catcgtttta tcttcatcag cggtgcatgt
      601 attttcggat tcctgtagtc ctttaagtcg cagatatctt ggcctgagaa gcttgtaaaa
      661 atctgtagcc tgctttttat atacctttgc aagagagttg tgaagctcct cttttgtgtt
      721 agactcagct ataagtttgc tgatctcttc tatttgctct tcgctgcaaa tgtttgcaag
      781 tatgctttgg agatcgttgt ttttttgtgc ggtacacttt tttttaccct ttttcacttt
      841 gacgatagtt tcaacaacag gatcactttc tgcttcgaca atatcaggcg cacaggcttc
      901 tggctcttct tcgacttctt ctgtattacc attcatattt tcgtttagtg taacagaata
      961 atctatagcc gcaggctcag ggtttggctc atcagcttca tcaagagtta tctctgactc
     1021 tgtctgaatt tctgctgttt cttctgtttc ctctgctttt tcgactatat gtttattcga
     1081 ttcttcatct tcatcttcat tgttttcggt cggattaacc ttatgattag cgaaattaat
     1141 agcggtttgt attgtcctag ttctgtagac catagcattt gggctatcaa catacttacc
     1201 atgccagaaa tcatgaagaa agtcaaaacc tctgtcattg cttataacaa aaatattcgt
     1261 ataagtatcc ttggctacca gataacccag aagtgttgaa agctgaaaat ccagggcatt
     1321 tttgccacca acgtttacct tgaagtattc aatatcagcc tttgatgtaa gaactttctg
     1381 gtgcatttca aagttaatgg tatcggaatt ttcactgtaa aaaattatga cggtatcagt
     1441 ttcagttaaa ttgtctatgc cttgaagtcc tttggatttg acattttcaa agtctaccaa
     1501 atatattttc atgttgatct atcctttctt tttgtgtcgt ataagaatcg tagctgcgat
     1561 tataacaata agtccgcctg tcgcagttgc tgtcataagt atggcatttt tgcctttttc
     1621 atcgttctca ctgctgtcag gaacgacctc ctcataggta taagtttttg tccttaacgc
     1681 cttgtctata caagaagcaa taagtgcatg acctttttta ttagggtgta tatcaaagtc
     1741 atttatgttg gtcagttcct ttccgtgacc gctgaattca ctaaaaacat ctgccactat
     1801 gtatctctca ttttcatctt cgtcagtgga gtgttctttg ataatgttgt taagttttga
     1861 aattttatcc tttgatatac tctggaaaac agcagccgca gtgaagttat caagcgggtc
     1921 atatagtgtc tgaacgatta tttcaccctt acttttggct tcaacagcgt taattatatc
     1981 atctagagtg ctcgtaaaat ttttcagcgc cttatccata tcatcagaca tatccttcat
     2041 atccattgca atgccaataa tatccttggt tttatccatc aaatcagaca atgtgctttt
     2101 agaagtaatg cccagatcgt tcatcaaaaa atctataaaa agaccaagaa tgtcattgcc
     2161 acctatagat ataataacag cgtcgctgtc tgcaaggtca gcgtcaaatt cgcctgaatt
     2221 aatgcggtca agaagctgct gtgaagtgtc gcccacaaca gcagagttca caagcttaca
     2281 gccgccaacg tcattaagct cagcgtcata ctggtcgtga agaagatttg cataagacgc
     2341 acaagaatat ctgttttcac tgtagccttc cagaccatag ccagcggcga ttgaatcccc
     2401 aagtacaagt atattaggcg gaacatttac ttcatgcttt ataacttcag cgttacaggc
     2461 aagaggtgtg gcacatgcca taacggttgc cgtgaaaaaa gccgttaatt gttttaacat
     2521 cttatcatct cctatatgag tatattaacc ttattatata atatattgta ccacattcag
     2581 gacaattgta caacatattt tttgaaattt cttgactttt ttagtgtgtt gtgataatat
     2641 ataataaaag aatatgaaat cagtttggaa agcgagattt attatgactg actattatta
     2701 ccctgtcctc agcgatgaaa cgagcctgcc tttgtatgtg cttggaatcg gtgcgagaga
     2761 tagagaattt cattttgtaa gagaagatgg ctatcctaat catcagataa tatactgcac
     2821 tcgtggtcag ggtcacatta tatttgatgg tcatgagttt gatataaagc cgggggacgc
     2881 attttatctc ccgccttctg tgccgcatga gtattatcct gttggtgata tatgggaaac
     2941 ccactggata acctttgagg gcagggaagc aattgaaatt ctcaagcata tcaagcttga
     3001 gaaggctaaa gtgtttcaca taaatgatat caatgctgtt gatgccatat ttaagaagat
     3061 actttatctt atgaagacta attactacta ctgtggtcac cagtgttcgg catatctata
     3121 tcagtttctt attgagctac acagagtcgt aaacctacaa aatggtagtc aagacggaca
     3181 aaaactcaat cagctgcagc ctgtaattga ttatattgat aagaatttca gaaaagaact
     3241 tacacttgtg gagcttgctg atcttattga cctttcaccg cagtatcttt gcagactttt
     3301 caaggagtgt cttagtctaa gaccttttga gtatttagca agaaagcgta tacagcaggc
     3361 aaagctgctt ttattggagg ataagcttaa catcaatgaa atcgctgcgg aggtaggtta
     3421 taacgattgc agttattttt gtgcggtgtt caaaaagcat gaaatgcttt cccctgcaga
     3481 atttcgttct cttcataaaa aagcaggtaa ttaaaaatat gatgnnnnnn nnnnatatga
     3541 tgccccacgg aatttgatcc gtggggcttt ttttataata tagttccgcc tccgataacg
     3601 tattcaccgt catagaaaac cactgcctgt ccctttgaga tagctttatg aggttcttta
     3661 aaagtcacga gtaccttgcc atcattcatt ggtgaaactg tacattcagc gtccttttga
     3721 tgataacgtg tctgagcagt gcaggttata ggctctgtga gggtgtcaaa cggaataaag
     3781 ttcacatctc ctgccgcaag gctgtcactg tatacttcat cagcctttcc cataataagc
     3841 ctgttatgtt ccatatcttt tgtaacaacg aaaagcggtt cgctatatga aatgcctacg
     3901 ccctttcgct gtccaatagt atagtgtatc agacctttgt gtttaccaag ctttgtgccg
     3961 tcagaaagaa cgatatctcc cgactgctgt tcaccgcagc gttttgtgat aaagcctgca
     4021 taatcaccgt cagggataaa gcagatgtcc tgagagtcag gcttgtttgc atttataagt
     4081 ttgttttctt ctgcgatatc acggatcttg tccttgtgca tctcgccaac aggaaagaga
     4141 gtgtgtgcta actgctcttg cgtcatgctg taaagaacat aactttgatc ctttgacctg
     4201 tcaggtgagc gtttcagaag ccacctgcct gaatttccgt catattctct ggcagcgtaa
     4261 tgccctgtag ctatataatc aaagcccagc agctttgcac gctcatagaa ttttgcgaat
     4321 ttcagatgtt tgttgcactc gatacacggg ttaggcgtgc cgcctgccag atatgtatca
     4381 acaaaattgt cgataacata gtgcttaaaa tcatcagaaa aattgaatac atggaaatca
     4441 atgcctagtt taaaagcaac aaaacgtgcg tcctctacat cattgagaga acagcacgtt
     4501 cttgttttat ccagaccgat gtcctcattg ctgtagagcc gcatggtagc ccctgccacg
     4561 tcataacctt catcaagaag gagcttgacc gaaacagagc tgtcaacgcc gccgctcatt
     4621 gccacaagaa ctctatcagc cattgcagtt ctcacaatca ggagcagact gcttacctac
     4681 gaacggagtt ggatcgatac cctgtctttt atagtagtca gaaaccgcag ccttcatagc
     4741 ctcttcggca agaacagagc agtgaagctt tgctggtgga agaccgtcaa gagcttcaac
     4801 aaccgccttg tttgtaagtt tcaaagcgtc ttcaagcttt ttacctttta tcatttctgt
     4861 agcgatagaa gatgtagcaa cagccgcacc gcaaccaaaa gtcttgaact taacatcagt
     4921 tataatgccg tcattgacct tgatgtacat tttcataata tctccgcact tggcgtttcc
     4981 tacctcgccg acaccgtcag catcttcaat ctcgccgaca tttctcggat gcgcgaaatg
     5041 atccattacc ttttcgctgt atatcatttt ttattcctcc taatcaagtg tatagtcgtg
     5101 aatattctca gttctgcaaa ttctttccca aagtggagac atacttctca ggtgctttac
     5161 gataggtgga agaacctcaa gaatataatc aacatcttcc tctgtgtttt cgtcattgat
     5221 agaaagtctg agcgagccat gtgctacttc gtgtggcaga ccaagtgaaa gcagaacgtg
     5281 tgacgggtca agtgatccgc ttgtgcaggc tgagcctgat gaagctgcaa cgccttgaag
     5341 atcaagtgaa agaagcaggc tctcgccttc tactccacga acagatatgt ttacgtttcc
     5401 tgcaagtctg tgttctgtgt caccattaag tcttgactca ggtatcttga gcagaccctc
     5461 gataagtcta tctctcatct ttctaacacg ctcattcttt gcagggatat ccttcacagc
     5521 atcagtgata gcaacgccga gagccacaat agaaggtacg ttctcggtac ctgcacgctt
     5581 atttctctcc tgcgctccac cgtagataag gttaggcagg ttaatgcctg tttttacata
     5641 aagcgcgcca acgccctttg gagcgtgtag cttatgtcct gaaagtgaga gcatatcaat
     5701 gttcatatct ttaacattga tgtcaatatt tcctacagcc tgaacagcgt cagtatggaa
     5761 ccatacgcca tgcttgtggc aaacttcgcc gatctcagcg ataggctgta tagtaccgat
     5821 ctcgttgtta gctgtcatta ttgtaacaag gcaggtatcc tcacggatag cgtcctcgac
     5881 ctgttgtgca gtaacaagac ccttgtcacc tacaggaaga tatgttacct caaagccaag
     5941 cttttcgaga tactgacaag tgtgcagaac tgcatgatgt tcaaaagcag ttgtgatgat
     6001 atgcttcttg cccttttttt ctccaagctg tgcacagcct cttatagccc agttgtcaga
     6061 ctcagagccg ccagcagtga aatagatctc attgaccttc gcaccaagag cgtttgcgac
     6121 ctgttctctt gctttaagaa cggcttttgc gcattccatt cccaaagaat atatacttga
     6181 cggatttccg tatagctcag tgaaataagg cagagcggcg tcaagagctt tctgagatac
     6241 tctagtcgta gctgagttat ctgcatagac aaatctcttt tccaattaaa tcataccttt
     6301 catatataga tttactattt tattatatac ttatttacta caaaatgcaa gtgatttcgt
     6361 taatttaaca gaaatttaac ttctactgaa tttctttgag cccatgactg caagttcgtc
     6421 acaacgttcg ttttccggat gtcctgcatg gccttttacc cagttgaaat tgaccttatg
     6481 cacctccagc agatcaagaa gcttttccca cagatcactg tttagagcct ttttcttgtc
     6541 cgattttatc cagttgtttg cacgccattt ctttgcccag ccttttgtaa cagcatcgat
     6601 tatatacttt gagtcgctgt aaagctctac ctcacatggc tctttcagtg ccgaaagggc
     6661 agtgataaca gcggttagtt ccatgcggtt attggtagtt gaagggtcgc cgccccaaag
     6721 ctcttttgat gtgcctttgt atcttagtat cgcaccgtat cctccaggac cgggattgcc
     6781 tgaacaagca ccgtcagtga aaatctctat tttcttcatt tatttatctc cgtttttatg
     6841 atgattatta tgcaaaacat attatactac atttctctct ttttttcaag tacctctcga
     6901 ggtttaatat tttaatattt tgtagtgcta caatagatat tggacaaaat taagaaaaaa
     6961 gaaaagagag atag
//
LOCUS       DAWEMR010000138         6972 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4383_length_6972_cov_10.541709, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000138 DAWEMR010000000
VERSION     DAWEMR010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4383_length_6972_cov_10.541709"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1793 a         1595 c         1447 g         2137 t
ORIGIN      
        1 cttccatatt gagggaatcc acgggctttg ttgcgctttt ttttacagcg cctttgtttt
       61 ttattcccac tccaccgttc ccggtggctt tgaggttata tcgtaaacta ctctgtttac
      121 atggtctact tcgtttgtta tcctgttgga tattcttgcg agaacatcgt atggtatctt
      181 tgcccaatcg gctgtcatga agtcgtctgt tgttacggct ctgatagcta taaggtggtc
      241 ataggttctg tggtcgccca ttacgcctac tgtctggacg ttcgggagaa ctgcaaagaa
      301 ctggctcagg ctgctttcaa ttccgctttt cttgatctcg tcacggaaaa cggcgtctgc
      361 ttcctgcaac acctttatct tatcctctgt tatctcaccg attattctta cgccaagtcc
      421 tggacctggg aatggctgac gccatacaag ctcctttggt atgccgagct tttcgccgac
      481 ttttcttacc tcatctttga aaagatcgcc aagcggctct ataacgccgt caaagttgat
      541 gtcctcaggc attttcacct gattatggtg agtttttatt gtagcagcgt cgccctttcc
      601 gctttcgatt cggtcaggat agatagtacc ctgtgcgaaa aatccgccgt ctgcctcttc
      661 tctgatctta tcccagaata ccttatagaa ctctgttccg atagtctttc tcttttcctc
      721 tggatctgtt atgcctttga gcttttcaag aaactgcttc tgacagttta ctcttacaaa
      781 tctcatatcg aaaagtcttg tgaaagtttc ctcaacaaag tcgccctcgt cttttctcat
      841 aaggccctga tctacgaaca cacaggtgag ctgtttgccc actgcacggc tgataagtcc
      901 tgcggcaact gatgagtcaa ctccgcctga aagtcccaaa actactttct tatcgcctat
      961 ctgctcttta agctttgcaa ctgtgttctc gataaagtta tccattaccc aatcgccttt
     1021 acagccgcag acctcataga gaaagttttt caaaatgacg ttaccatact cactgtgagt
     1081 tacctcaggg tggaactgaa cggcatatat cttcttctct gcattctcca ttgacgcaca
     1141 tgggcaatca tctgaatggg aagtgataac aaagccctca ggtgcttcgc tgacatagtc
     1201 ggtatggctc atccatacga tattcttatc aggaacgtcc ttgaaaagct tgctctctgc
     1261 ggaggctgtt atctctatct tgccatactc gctcttttta cagctttcta cctttccacc
     1321 gctcatccat gaaataagct gacagccgta gcagatgccg aggactggta tgccaaggtc
     1381 aagtatagcc ttgtcataat gcggggaagt catatcataa acggagtttg gtccgcctgt
     1441 gaagattatg cccttatagc ctgccgcttt tatttcttca agcggagttt caaagctttt
     1501 tatctctgca taaacgtggt gatctctcac acgtcttgcg ataagctgat tgtactgtcc
     1561 gccaaagtca agcaccagta tcttctcatg tgacatatat ttacctcttt tccttatgtt
     1621 ttttgaaata cataaatatt ataacacgtt gccgtccctt tttcaagagc aatttacaca
     1681 ctctttgaag catggggtaa attaattgtg aacacctgac atcaaacagg aaaatattcg
     1741 ttgattttag ctgttgtaat ttgctaatag ctatgttata atgaataggt attgtaaaat
     1801 gcgtgtaaat caacagaaaa gagtatattg taatggaaga acaacaaaac attcaaacac
     1861 agcaggcaaa ggataaaaaa ggcttgctga aaaaaatagg agcatggttt gcgtcgcctg
     1921 acggaatgca aacattacga tttataagtg ccatagcggc agttatagcg gtaggagtca
     1981 tctgtgcttt gttgggcata tacatggaca aagacaactt cctgccgaaa gaacgaaatt
     2041 atgcgcttat aggggcttgc gccgttgagc tggcactggt atttattatt gcgaaatttt
     2101 tctcaatgca aaagaagctg aaaaagagcg aaacaaaagg ctgcatcgac cctttcacgg
     2161 cggcttttat gcttggacta aattcttatt ttcttccgga ctttgtaatg gggtcatatt
     2221 ttccaaagag caatgtgctt tacattctcc ttggaatatt gctctgctgg cttttatatc
     2281 ttataggtgc ggtgatattc ccgaagccac acgcctggtt ttgcggagtg aacgtcatat
     2341 ttgccatata tgctttggca cagtattatg tcaccgagtt cagggggaat cctgttcagt
     2401 ttgccgacct tgccaacata aagtcagtca gtgagataaa cggaatgtac tcactgtttt
     2461 tagacggcaa cgtgatgttt gtgctttgtg atcttattct tattttcgct gtaacggtga
     2521 cgacaaaagt aaggaagata aaaatacgca gcaggataat atcgggtgtg gctgttatcg
     2581 cagggtgttt cgtattcgta tacggcggca ggtttgccta tgaccttggt ataaagaaca
     2641 gatacatcag gctgaacttt tcaggtgcgg aaaatgcaga cacatacagg tgcgtaggct
     2701 atgaccttat gttctgcttt gacggaatgt ttaaccgtgt gacgaagcct gatggctact
     2761 ccactcaaaa ggcagaggat atcataactc agtatgaggt acaaaaggct gacaaaaagc
     2821 ctactatcat tgctatcatg aacgaatcct ttgcagactt tgagcatatc gctcagttca
     2881 agactaacaa agactatctg cccaactatc acaaattgca ggaggaaagc ataagcggat
     2941 atgtttccgt ttcggcatat ggcggttatt cctgcaactc tgagtatgaa tttctcactg
     3001 gcaacactct tggctttttg ccgtcgggaa gtgcggtatt cacccaatat ctcaacgaca
     3061 aacaaaacgg tcttgtaagc tggctaaacg agcttggcta cacaacgcag gctatctcgc
     3121 cttgctctga gggactatgg gatataggca acgcctatga acagcttcaa ttcaaagaca
     3181 ggatatacaa ttgtcaggac agaatgagcg atatccaata tttcaacgga aatatcacag
     3241 atgaaaccat tttcagatat attgaagagc aatatgaaag acacgcaggc tcgccttatt
     3301 ttgtatggac agcgaccatg cagaaccatg cgccgtatga tgacctcagt tctgttccgt
     3361 ctgagataac ctttgaggac tacaacaacc ctgcggcaca ggaatatcta aatcttatct
     3421 atcagtctga caaggcgttg ggagagctta taaactattt ccgacacaag caggaagagg
     3481 tcataatcgt aatgttcggc gaccattatc cgcatatcat aaacttcacg gaatatctct
     3541 atggcaagga tgtggcttta ctttcaacgg aggattactc aagacttcga cagacgcctt
     3601 tccttatttg gagcaacaag ggcgttgaag cggagaagat agacgatata agccttaact
     3661 atctttcaaa cgaggtcatg gaagcggctg ggctaccaaa gtcgagatat cagcaggagc
     3721 ttgacaagat acgctgtgac attccgataa taagctctta cggctacaag acggctgacg
     3781 gagaatggta ttctataaat gatgtggctg acaaatacaa ggacacgctg aacgaatatg
     3841 aaactgtgca gtattacaga atgtttgatg aaaagcgtga caatagtgta aattaggctg
     3901 ttaaaaaaca caaacagcaa aaaagtggga cgattcccta caaaacaata aacggacgaa
     3961 gcttttttac ttcgtccgtt tcgttttata atgcgtgtca aaatgcgggc gaacactgtt
     4021 cgccactaca aataactaca taattatgcg cttatttttt tatcctgtga cctttttctc
     4081 gaacatgaac attcccatga catcgttcat tatgctgtca gaagcgacct tcttgctgtt
     4141 ctttgtcatt tgccttacga cagtctgacc gtcattcttg ccgaatatca taacataatc
     4201 gtcgccactt tcggaagttc cctcatagga cacctgattt ttaaagatgt tatatttcca
     4261 cttgccgtct ttgaaatatg cgtcaacggc ttcgccgtaa ttttcgcttg ccaatttcat
     4321 ttcggtatcg aaatcgtagg tggaatgtct tacgctgaat attctcacaa aggcgatcac
     4381 aaaatatatc accagtatta ccactgtgat cttaaacaga gtctttagta tctttttgcc
     4441 cttaccataa ttctctttgc cgctgcttga atgttcagtt ggggtgcgcc tatagtctgc
     4501 cgcactcatt tctctgtttc ggtctgtctc tcttacacgc tctaccctgc gggcttcctg
     4561 ctctcggcgc attctttcct ctgcttcggc tcttttcggg tgttcgtaaa cacgcttcgg
     4621 tatgccctgt atccttgaac ggtctataaa ctcctgctcg ccacgtcttt gggtcgatga
     4681 ggcgataggt accatttttc cgcagttggt gcatatctta gctccgtcct ccagcttaga
     4741 accgcagaat gtacaatgtc tgctcattat taccaatcct ttacacactt agaactgttt
     4801 tgtttgtcga agttttttct atatctatgt tggactatcc gcccgttttc tttaatgaca
     4861 cgggcgaacg ctgttcgccc ctacaaatct tctttatcta tatttacaga tatcttacaa
     4921 cgcccacaac cttgccaagt atgcttactt cgtctactat gataggatcc atggtgtcat
     4981 tttcaggctg cagtctgtaa tggccgtcct ctttatagaa agtctttatg gtagctgact
     5041 cgttgtcaac aagtgcgcag actatatcgc cgttttctgc atatggggtc tgctctataa
     5101 ctaccatatc tccgtcaagg atagcggcgt ttatcatgga ctctcccctt acctttagag
     5161 caaaaagcgg attgctgtaa tgccttgcag gctggaagct tatatagtct gttatctcct
     5221 caatggcagt gatagggata cctgcggtga cagttcccac aagggggatc ttcataccgt
     5281 tgccgcctgt gagccttatg gctctgttct ggttatttcc cttttcaaga agaccttttg
     5341 cggtgagatt atttatatat ctatgggctg ttgaggtaga cttaaagcca aattccgcac
     5401 aaatctccct aactgtcggc ggatatccct cttctatgcg atcctttatg aactcgaata
     5461 ccattttctc gttctcggtt acttttctca tagctccgtc ctttctgtta tatcaataaa
     5521 tctttatctt ctaaacttaa agctcttttg aaagcctttt tgcaagctta tagacatctc
     5581 cgcagccaag agttataaca aggtcgccct ctttgcagtt tttctttata tagtcgatac
     5641 actgtgagaa gttatattcc ttctgctgtg cagtctgctt gtcaacaagc tcgtggtcgg
     5701 tcttgaacca aactgcattc ggtatcttct ctgcaagctg ttcagcataa acaccgtcgg
     5761 tatttttctc acggcttccc atgatatccg tgataacggc aatatctgca atggaaagcg
     5821 ctttcacaaa atcgttcatg agtatctttg ttcttgaata ggtgaaaggc tggaaaactg
     5881 cccacactct gttgaaatca agaccctttg cggctttcag tgtacactcg atctcctttg
     5941 ggtggtgagc gtaatcgtca acgatagtaa cacccttgac ctcggctatc ttctcgaaac
     6001 gtcttattgc gccaaagaaa gtatcaagtc ccttttcgat accctcccat gaaacgcctg
     6061 aatatcttgc ggcggcgata gctgcaaggg cgttcatgac gttatggtcg ccaggaacat
     6121 ggatagtaag ctctctgaca aactcgccct tataataaag ctcaaagcgt gtctgcaagc
     6181 cttttttgga ctttataaca gcagaataat cgtttttctt atcccagccg aaagatatca
     6241 tttccttgtc gacaccctca acggctttca gagtatttgc gtcgtcgcca ttatagataa
     6301 cggcttttga agccatggtt gcgaacttat ggaaagactt tatgatattt tcgatattct
     6361 tgaaatactc aaggtggtct gcgtcaacgt tcaggataat tgaaacgtca gggaaaagtc
     6421 tgaggaaatg atcctcgaac tcacaggatt cacagaccat agtctgtgat gagcctgcct
     6481 tgcctgaacc gcctatgctt gggagctttc cgcctataac gcaggaaagg tcagtaccct
     6541 cctgaacgaa tatctgtgtg agcatggaag aagttgtggt ctttccgtga gtgcctgaaa
     6601 cgcaaagagc gttcgtatac cagcttgtga caagtccaag tatctcggct ctttcacagc
     6661 agatcgcacc gcttgccttt gcggcgataa gctcagggtt atctgccatt attgcggcgg
     6721 tcttgagtat aaggtctgca cccttgatct tctcggctct ctgaccgaga acgacttcta
     6781 tgccaagctt tcttacggct tcaagagttt cagtttcgtt attgtctgag cctgtgatga
     6841 aatatccctt tgaatggaat atctgcacca gagggtacat accagagccg cctataccaa
     6901 taaaatgaac gtgctttata tttttcaaga tctcatttgt tatcatcttt gtaatagtcc
     6961 ttagactaat ag
//
LOCUS       DAWEMR010000139         6968 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4387_length_6968_cov_11.691885, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000139 DAWEMR010000000
VERSION     DAWEMR010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4387_length_6968_cov_11.691885"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1923 a         1465 c         1524 g         2056 t
ORIGIN      
        1 caaaggatca taatagccgc acttattctg ggcatgatgt tttgcatagg tattacgatt
       61 tgggctgtgt ttttcagaaa gccagaaaca aaatctctag tacctgatta tgcacctcag
      121 cagacagagc agaatgctga acctttggag gacaacagca gaagtcttga tgtgcctgag
      181 ggtggcggag gaatttctct tgaatacgaa aatacagtga atatcaagct ttctgataaa
      241 acagcttatt ttagttataa gcatccaagc agttcaactc aggatatcgt gctttgtatc
      301 gaggtaaatg gtgagatcat cgcacgctca ggtactataa aaccaggtaa tcagcttaaa
      361 acgcttcctt tgcttgaggg cgaggcggac aaacttagtg aaggcactta tacagacgca
      421 aatttcagag ttttaagtta tgatccgcaa tcaggtgaaa aagcaatggt cgatacggtg
      481 gcaaaaataa ctgtcactgt ggaaaaataa ataacggagg aatttatcat gaaaaacaaa
      541 tttgcgatca ttgctgcatt agtgcttgca agcactagcg tggttgcagc atatgcggta
      601 aattcagaaa aaaatgacgg cagcgaacca acaaatatca aggttacagg aagctatatt
      661 gattccaagt caacgacaat gtctgtggac gttgagtggg aggaaatgaa ttttacctac
      721 tttggcggac ataaggtatg ggatcctgaa acacatagct ataccacaga gtctgcatgc
      781 tggtgggatg agcctaagga gataactgta acaaaccatt caaattcggc tattaaagca
      841 cagttgaatt ttgcatcaaa ggtcgaagga cttaacggca tgttttatga agatgttatt
      901 ttattggaaa cagccgaagg cactactgtt gacgctgcac ctaaagattc aacacatttc
      961 agtgtaagtg gagcagggat agaaacaaac aatacagatc ttggcactat cacagtaacg
     1021 gtctctgaag acattcaata agtaactgac gcaatggttg cttctactca tgttgataca
     1081 actctttatt atgtaacagg atcaaatgga gcatggaggg tagtaagaaa caatgatatt
     1141 gggattgtac cgatagcgta ataaaaaagt attcatatca gggggcttat gatggataag
     1201 caaaaaaatg acgggaagct gatcgtatca gtgcttatgg tctgttttgc agttaccatt
     1261 ggcgtgatga tatttgcatt gtgccgtcca aatgataaaa aaagtgcaga atttactcca
     1321 ccgccttttg aaaacacagc acagcatggc aagcctgagg tttcagaaaa tttaggctat
     1381 agctctccga acagagatga tatgagctat gattttgcgg tgtgcggaaa cgtaactatg
     1441 gaaggcgata aggcaatagt atatcttaca aatatcgagg gcaacaatgc ctggataaag
     1501 ctcagagtgt ttgacgaaca gggaaatatg ctcggagaaa cgggtcttat caagccaaac
     1561 gagtatgtga aatatgttac gctctcaaag acgccggagg tcggtacaaa gataaagctt
     1621 aagatcatgg gttacgagcc gcacacttat tatagtgcag gtgcggcttc cttgaataca
     1681 cagatagggg gtcaaataga ccaatgaaaa aaatgacttc atttcttgct cttgtgcttg
     1741 cattaagcat accttctggt gcttatgcgg ccgaggcaaa gcttgatgat gataatacaa
     1801 ctgcaaatgg aaatgtttat gcggtctata cagaagatgt tccatggaac agtgtgcctg
     1861 ttgacgacga tggaaatgct gttattgatt ttccagatgg cacaagcgtg aagatcgaga
     1921 atgccgataa agataaaaat cttgtggtgg atcaggtcac agagaaagaa gctcttgact
     1981 ggttaaacag cgtcatggga aaaaagtctg aaaacaccgt agcatttcat atttattaca
     2041 ttgacggaga ttctattctg ccggcaaatg gaataaaggt aacgataaac atagacaaag
     2101 atcaggtaaa ttccgttaaa gctgatggta cagtaagcag tcttaaatgt gaagagtctg
     2161 atgcagaaat atcatttgtc acagacgatg cacctttcta tgtccttggt acaaaaacac
     2221 ctgaaagttc atcagatata tcaccagaca gcactggtaa aaaatatgat agcttacaaa
     2281 acagctcaca gacagcagtt caaaatgtaa acaaagacgg tgataagtca cctaacacag
     2341 gcttaggagc aggtgcagca ttgacaatgt tgctggttgg tgccgttgcc gtaagcgtgt
     2401 caaaaagaac aaaggataaa tgatctgata ttcataaggc tcgccgtttg cattggcgtt
     2461 attaagcaaa aaaagactcg cacgaaattg tgcgagttct ttttttatgt agcagatata
     2521 taaaatacac ttatatttca atataatccc aaagctcgga aacaagtttt tctgtaaagc
     2581 ccttgcacaa aagaagctcg ttagggcttt cgtaatagga tataagctca cgaagttcta
     2641 tgacagcatt cgccttgtcc atatcaatta gcagcttgtc tgatatctcc tgagcgtcag
     2701 ccttgtttat gttcactttc tgcctgactt tctcagtagt ttcagccgtt gtaacagttt
     2761 gctcctctgt ggccggaggc tctgttgcag tatcagcagg gatttctgtt tgaacaggct
     2821 gagtttctgc gactttcgtt tcctgcggca tatcatggta gtctgcactg tcaacataaa
     2881 aatagccttt gagaagtgca agtgtagagt ctccgatgcc gtttacttca agaagcatat
     2941 ccatgttgct gataacacca actctgtcac gatatgctat tatcctgtcg gctgttacct
     3001 cgcctatgcc gctcactgcc atgagcattt ccttgtcagc tttgtttatg tcagcaggaa
     3061 agctgtattc ctctttggtt tcagcaatac tgcttgtgac ggcttttgat gtcgttgttt
     3121 ttctggtggt gtgtgccttt gtagtcttgc taatgacctt ttcactgcct gaattgtcca
     3181 ccttgtcagc ctgaaagctg tcagcaggcg gtgtgagtat caccacatca tcacgctgtg
     3241 gtctgttaac aagtgcagaa gcgatcgctg tagtaatggc gatcgcaact gcgatcacgc
     3301 ctacgaaaag ttttattctg cttgttttta cttttgaagc atcattttct gtcacattcg
     3361 atttttcgtc tttgttgctg tctaacttgc tgtccataat tgattatttt tctgcaagca
     3421 tttcgttgag attttccacc atttctgaca tcttcgctct tgtgatgtct ggaacatttg
     3481 taaatgtaac aagggtcctc agtggcatat ctcttatcat tgcttccttc atttcctttg
     3541 cagaagcgcc aagatcgtcg gaagccgtgt cgccaaaacc gcagtctatt tggcttaaaa
     3601 tgtcctcgaa catagctctt ccctctggga tagaatttat ctcgccacag gtggtgttca
     3661 aggtgaaatg cactgggagt tttgtatcgg aagatacata aacagaagtc gaaagcctga
     3721 tatctcttga agaagagcct gcctcgataa taaactcacc atattcaacg aaccaatcgt
     3781 gtatatcagg ctcgtagtat gcaaatgaac gcttatcaag gtggaacaca gcctttttgg
     3841 tctcgccagc tttaaggaaa agcttttcaa agcccttaag ctcacggaca ggtctgcgca
     3901 ctgagctttc cttgtcggaa acgtaaagct gaacgatctc cataccgtct ctgtcgcctg
     3961 tgtttgttac gtccaccatg accgtcaggg tcttgtcgtc ttttatctcg ttttctgaaa
     4021 cagtaagatt gctgtattca aaggttgtgt atgaaagtcc gtgaccaaat gggaacagtg
     4081 gctttatgcc cttttcatca taatatctgt agcccacgaa aattccctcg gaatagtcaa
     4141 catcgtccat atttcccggg aaattaagat aagacggatt atgtgaaagc ttttcaggga
     4201 aagtttccgc aagctttccg ctagggttag cttcgccaaa aagcagagcc ttttctgcag
     4261 tgccgatgtt ctgtccgcca agatacattt caagtatgcc cttcacctta tctgcaaacg
     4321 gcatttcaac agccgagccg ttgtggagaa cgataacaac gttttcattt accttagcaa
     4381 cttcatcaat aagcttgagc tgacaatcag gcattctcat atgctttctg tcaaagccct
     4441 cagactcaaa gctttcgggc agacctgcga agataacggc aacatcgctg ttcttggcaa
     4501 gctccacagc ttcatcgaga agctcgtcaa cagtttcgtc acggtcggtg acgaagcctt
     4561 gtgcgtatgt gatattatcc atgcccttga cagcttcaag agcagatgtt accttaaatg
     4621 aatttatgtg agagcttccg ccgccctgat aacgtggctt gtcggcaaat tcgccaatga
     4681 aagctatctt tgcagacttg tcaagcggca gtatcttatc atcatttttg agcagtaccg
     4741 cacattcaga ctctatcttg cctgcaagct catgctgttt ttctttatcc cactttgttg
     4801 caggggcata gccgtcctcg gctttctgaa taagtgtaag cacgtttctc acagccttgt
     4861 caaggtcttt cattgaaagc gagccgttgt ttacggcttc tatgataagc tcgtcattct
     4921 tgccgttgct agtaggcatc tcaaggtcaa gacctgcggc aacgccaaga ggtctgtcat
     4981 caactgcgcc ccaatcgcta actacaagtc cttcatagcc ccattcgttt ctgagtatat
     5041 cagtgagcag aagcttgttc tgcgaggaat attcgccgtt tatgcggttg tatgaacaca
     5101 tcactgtcca aggctttgct tccttgactg gtgtttcaaa ggcacttaaa tatatctcat
     5161 ggaaagttct ctcatcaacg ttggcagaat agctcatacg tcttgtttcc tgattgttca
     5221 tggcaaagtg tttaagggaa gttcccacgc ccttgctctg aacgcccttt atgtgtgctg
     5281 tggcaagctg tgaagcaaga taagggtctt ctgagaagta ttcaaagttt ctgccgcaaa
     5341 gtggtgaacg ctttatgtta cagccagggc caagtataac tgaaacgtcc tcagcctgac
     5401 actcctcgcc aagagccttg ccgagagttg taagcagctt tctgtcaaaa gagcaggcaa
     5461 gtgcgcaggc acatgggaag catactgcct tgatagcttc gttaggattg tctgtatcat
     5521 cacgcatttt tctaagtccg tgagggccgt cagacaccat tatgcttggt atatccagtc
     5581 tttttatttc ctcagtgtgc cagaagtcag agcctgagca caatgaagct ttttcttcaa
     5641 gtgtgagttc cttcaaaatc ttgtctatgt tcattgtgaa aacctctttt ctataaaact
     5701 atagtttgat tgtaacacaa ttttctatga aattctatat attttgtgca tattttacat
     5761 aaagttaata ttattttcga ttagtttggt aagagccacc ctgaaatcct cgtcctgctg
     5821 tttggtgcgc tttggcggac ctttgagcct gccaccgctt ctgcgtatct tctttgcagt
     5881 atgtcttgat aggagaagtg catcaatgtt ggagctgtca tatatcctgc cgttctggtc
     5941 cagggcaagg acgtttcttg caagacacat tgccgcaaga ccataatcct gcgtcacggc
     6001 aatatcgccc tttagagcga tgttcacaag cttcatgtct gcactgtctg cacctttgtc
     6061 gacagttatc acctgtgcat agtcagagct gaaaatatgc gagctgtcgc aaactatgat
     6121 acattcaagc ccatggcttt tgcttataga aactgcgata ttcgtaacag gacagccgtc
     6181 agcgtctata agtatcctca cgccttgccg ccttctatac aaaatgtaga ttccatatct
     6241 gcaatgtggg tcgccagtgc gagaggatac atttcaaggg caagaccgat actgttcttg
     6301 ttttcttctc ctgaaaagcc catgtgccag cgtatagcca tggcttcctc acgggtcagt
     6361 cgcatatagc tgcttactat gtagaccgac ttttcaccgt gaccatatgg cagtgtttcg
     6421 tgaaattcat agtatgggac tttctcccac acgccctgct cgttcttggc gttgcggtag
     6481 cttactctgt aaagatttac cttgcagatg tcatgaagca gtgaaacgat agcaatgctc
     6541 tcgtcagagt attccatgtt gtaatcttcc tttacacgct cacgctccag atagcttttc
     6601 aggcactcat aaacgtgtat gctgtgttcg cacaagccac cctcgtatgc tccgtggtat
     6661 cttgtagaag ccggtgcaac aaagaagtcg ctgttttctg aaagcagata attgagcagc
     6721 ttttccgaac ctgggcgctt tatgttttcc gtgtagatct caacgaattt gtctttaagt
     6781 tctgttacct ttgacatagt tgacctcctg taaaaatatt atattatgat aagctttagc
     6841 gttttagttt taccaaacta attataacat attttttgta agattaacag tacttttcgc
     6901 taatttttgc attttgtagg tttgtcaatc atattggaca ctattattaa gaaattcgat
     6961 aggcgaaa
//
LOCUS       DAWEMR010000140         6880 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4448_length_6880_cov_9.301245, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000140 DAWEMR010000000
VERSION     DAWEMR010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4448_length_6880_cov_9.301245"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    303..312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2795..2804
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3815..3824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1821 a         1452 c         1610 g         1967 t
ORIGIN      
        1 taggggacgg cgccctcgac gtcccgtttg caaaattgat aaagaatatg atatttctgc
       61 ggacgacttt tatgccgtcc gctttttttg gtattgtgta aaggtttaca tatatcttaa
      121 taaatttata taatgtaaat atcaattcat gaaatgttta cactaaaata tgctcaatcc
      181 attaaaatac ttgcacatat cactttttta ctattgcaat ttaggcaaat atgctataaa
      241 atatacataa agggggagaa acggacttcc cgataaaagt ttcatttttt tatattctga
      301 aannnnnnnn nngagggttt attacatgaa atttgctgac ggtttctggc tcaaccagag
      361 aggctacgaa gtaaactatg caaatcaggc tttcgagatc gaagagatcg aaaacggatt
      421 cctcgttgtg gcaacacctt tcgttgtata caacagagct atgatgctcg gaagtgcaaa
      481 ccttgagatc gcttacactt caactcagga gaactgcgta aaggtaagca tcacacacca
      541 caagggtcat gttgacaatg gtccgcactt tgttctcgag gacaagggct tcaagccaca
      601 ggtaactgtt acagacaagg aagctgtact tgttacaggc gacacaaagc tcgttgtttc
      661 taaggaaaac tggagcttca catactatta tatggataag aaactcacaa gtggtggctg
      721 gagatcgaca ggcgttattt ttgagaatgc ttacaagcag caggcaagac ttcagacaca
      781 gctcgacgac acattctgga gctaccctgc cgatcctaca agaacataca tcagagagca
      841 atttgacact tctgttggcg aatatttcta cggtttcggc gaaaagttca caacattcac
      901 taagaacggt cagaacgttg agatatggaa cgctgacgga ggcacttgtt ccgatcagtc
      961 ttacaagtct atcccattct atctttcttc aaagggctac ggtgtatttg taaacagcac
     1021 tgacaaggtt tcatatgagg tttgctctga cactgtttct aaggtatcaa tgacagttcc
     1081 aggtgagagc cttgaatact atgtattcgg cggtgctaac ctcactgacg tactcaaggg
     1141 ctacactgac cttacaggca agccttcact gccgcctgca ttctcatttg gtctgtggct
     1201 cacaacttca tttacaacaa catatgatga ggagactatc acatcattta ttgatggcat
     1261 ggctgagaga gatattcctc ttcaggtatt ccactttgac tgcttctgga tgaagggctt
     1321 tgagtggtgt aactttgagt gggacaagtc acagttccct gacccacctg caatgctcaa
     1381 gagacttcac gaaaacaagg gtctgaaaac ttgcgtatgg atcaacccat atgttggtca
     1441 gcgttcaaag ctttttgatg agggtatgaa gaacggctac ttcatcaaga aaactgacgg
     1501 ctctgtattc cagtgtgatg agtggcagcc tggtatggct attgttgact tcactaatcc
     1561 tgaggcttgc aagtggtatg caggatatct gagaaagctc tgcgaaatgg gtgttgacac
     1621 attcaagaca gactttggtg agagaatacc tacaagagat gttgtttact atgacggctc
     1681 tgaccctatc aagatgcaca acttttacac atatctttat aacgagtgtg tattcaatgt
     1741 actgaaagaa tattttggag agaacaaggc ttgtctgttc gcacgttctg ctacagcagg
     1801 cggtcagaag ttccctgttc actggggcgg agattgctca ggcaactaca actcaatggc
     1861 cgaggttgta agaggctgtc tgtcactttg tatctcaggc ttcggctaca cttcacatga
     1921 catggcaggc tttgaagcta ctgctacacc tgacatttac aagagatggg ctgcgttcgg
     1981 tatgttctca tcacattcaa gactgcatgg taaccagtca tacagagtgc catggctctt
     2041 cgacgaggag tcctgcgacg ttctgcgctt cttcacaaag ctcaagggca agctcatgcc
     2101 ttatctgtgg gctcaggcta tcaagacaca cgaggtcggc gtacctgtaa tgagagcaat
     2161 ggtcatcgac tttgcggacg acccaacttg cctgacgctt gacagacagt atatgttcgg
     2221 cgacaacatt cttgttgctc ctattcttaa tgacgagggt atcgctgaat actatgttcc
     2281 agagggcaag tggacagata ttatcaccgg caaggttctt gagggcggca agtggtataa
     2341 gcacaaatgc tcatactttg agatacctgc tcttgctaag ccaaacagca ttattgcata
     2401 tggcaacttt gaaagacaga tagagtatga ttatcttgag ggtacaacat tcactatcta
     2461 cgagcttgaa gacggcaagt ctgctgtttg ccctatttat gacactgagg caaacaaggt
     2521 attcgagctg acagctacaa gacacggcaa caagatcgaa tgcgaataca ctgacacaaa
     2581 ggcaagcttc aagatcgctg tggctaacac agacaagact gttgaaattg ctcctaactt
     2641 cagtggcaag gtcatcattg aactctaatg actaaataaa actatcgctc cgagagatcg
     2701 gggcgatttt ttttgcgttc acacataaaa tcggggcaaa tgagggtaaa aattctgtat
     2761 atttgctctt gaagaaaata tgagatcggg gtatnnnnnn nnnngcaggt gggatatctt
     2821 tgcggtttca agggctttga aaggtctgag aaagtgggag aggatattgc tgacgaatgt
     2881 cttatttttt cgggcattga cggcaggtca cttgacccaa tactgaaaaa gctcagggag
     2941 aaaggttcag cagtggaact gaaagccatt gtcacggcac acaatcaaaa gtggaacgtt
     3001 ggcgagctta taaaagagct gcggcgtgaa cacatttaca tgaacggagg tgacagagat
     3061 gaaaagtaaa tacaaggcta ttatttacat tattatgtcg gctttttcgt ttgcattcat
     3121 gttcgcattt gtgcggcttt cgggagactt accgacggtg caaaagacct ttttcagaaa
     3181 ctttgtggcg ttgtttttcg ctattggagt gctaaagaaa aatcacatac gctttcacat
     3241 tgatggcaaa aagaacaatg cggtcatttt tgtgagagcg cttgctggca cgctaggaat
     3301 gcttggaaac ttctatgcca tagatcatct tgtgctgtct gacgcttcca tgctaaacaa
     3361 aatgtcaccg tttttcgtta tcattttctc ttttattttc cttaaagagc ggctgacttt
     3421 ctttcagggt ttcactgtag tttgtgcatt tataggaagc ttgtttatca tcaagccgag
     3481 ttttcagaat gtggacttct tccctgccct tatcggtttt ctaggaggaa tgggagcggg
     3541 acttgcttac gcttgcgtaa gatacttagg acagcatgga gtaaatggtg cggtgatagt
     3601 tgcatacttc tcgggatttt cctgcttgtt caccctgcca tttctaatat tagatttcca
     3661 ccctatgacg attttccaaa tactttgttt attgggagct ggcgcaggtg cggcaggcgg
     3721 gcagttcgct ataactgcgg catacactca cgctcctgca agggagatat cggtttatga
     3781 ttactcgcag ttgattttta ccacgatttt gggannnnnn nnnnttttgg agatattcct
     3841 gatggctgga gctttcttgg atattttatc ataattgcca tggctgtgct tgtttttctt
     3901 tacaacaatg tttggcacaa tgacagcgag gtcaagggct gacaaaaagg caaacaaaaa
     3961 agagcgtttt tactttctcg gtaaaaacgc tctttattat ttatattgtg gtttaagctc
     4021 cgcctgcgat aaatctcttg agatcctgtg ggtttacgca cttttcaagc gcaacgtcca
     4081 tggagatcgt tctcgaacga acgagctttg caagctcctg atccatggtc tgcattcctg
     4141 ttgctttacc tgtctgcata gctgaaccga taagatgcac cttatcgtca cggatcatag
     4201 ctttgataga gtctgttgca ttaagaatct cacagcaagc tacacggctt gtgccgtcga
     4261 atgtacggca aagtgtctga gtacagatac ctacaagaac ggaagcaagc tgagttctga
     4321 tctgatgctg ctggtgagca gggaaaacgt cgatgatacg gtcgatagtt gacggtgcgg
     4381 acgttgtatg aagtgtactc ataacaaggt gtcctgtttc agcggcggta acagcagctt
     4441 cgatagtctc gaagtcacgc atttctccga cgaggataac gtccgggtcc tcacggagag
     4501 cagcacggag agcaagtgag aagctcggta cgtcaggtcc gatctctctc tggtttacca
     4561 tacatctctt atgagcgtgc atatactcga tagggtcttc gatagtgatt atatgagctg
     4621 atttatggat atttacatgg tcgatcattg cggcaagagt tgttgactta cctgaaccgg
     4681 tagggcctgt tacaagcaca agtccacgag gtctcattga gaactctgca aggataggtg
     4741 gcaggtcaag gtcggtaagg gtcggaatat cgtttctcaa aagacggaga gcgatggccg
     4801 tgtttcctct ctgacgatac acgtttacac ggtgacggaa acctcttgaa gatacatatg
     4861 taaagtcggt atcaatatgg tctttgatag actgacgctg cttatctgta cacatatcct
     4921 cgcatatttt gagaatttcg atctcggtca tatcaggata tgaggaaagc tttctcaggt
     4981 taccgccctg tctaactatc ggcggaatac ctacggtgaa gtgcaggtcg gaacagccaa
     5041 gtgctcttgc ttcatcaaga attttgttaa tgtcgattgc cattagaatt tacctccata
     5101 tatttctttt ttatacagca tatgttacac ggataagttc gtccattgag gtcttgcctt
     5161 gctgaacaag ctctgaaacg ttgtcacgaa gcaggtgaca gccctctttc tttgcaagct
     5221 cctgaatttc gtcagctttt gctccccttg tgataagagc ctgcatagac ggtgttgcga
     5281 gaaggatctc atggatagcg gttcttccct tatatcctgt gttattgcac tttggacagc
     5341 cacatgggtc gtacacctgt actgaatggt caagacccat aagctcgttt tcttcctcag
     5401 ttgacattct aggctttttg cactcagggc aaagcacctt tgtaagacgc tgagcaacga
     5461 taccgataag tgatgttgcg accatgtatg cctcaacgcc catatccaca agtcttgtta
     5521 cagtagaagc ggcgtcattg gtatgaagtg ttgacagaac aaggtgtcct gtaatagcgg
     5581 cacggatagc gatctcagca gtttcggtat cacgcatttc tccgaccatt atgatgtcag
     5641 ggtcctgacg gaggatagaa cgaagggctg caccgaatgt catgcctgcc ttttcgttta
     5701 tctggcactg gtttacgcca tcgatctttt tctcgactgg gtcttcgacg gtaataacat
     5761 tgttattagg ctttgagatc tcaccaagcg cggcataaag tgtggtagat ttacctgaac
     5821 cagtagggcc tgttacaagg acaacaccct gtggcacttt aaggcaagac tcaaatctct
     5881 tatagttata ctcggtcata cccaagtcgg ttatttttct aagggacata tcgcctgttg
     5941 cgaggatacg gagaacgact ttctcaccgt acaccattgg aaggttagat acacggaggt
     6001 cgagagttgt gccgtcaacg acctgtgaca tacggccgtc ctgcgggata cgcttttcgg
     6061 cgatattcat accgctcaag atcttgaaac gggtaacaag cgaagtatga agtgctgcag
     6121 aaagggtcat aagctcgata aggtcgccgt ttacacggat acggataacg gttttatcct
     6181 tgaatggctc gatatggata tccgttgcat cctgacgctg ggcgttctcg acgatagcgg
     6241 tagcaagttt aacgatagga gcgttctcga ttctctcctc ggagatatcc ttgagttctt
     6301 caaggttaag ctcttcctct ttttgctttg tagcttcgtc aacaacggag ttaacctgac
     6361 cttctgagta catcttacca atagcacggt tgatagatga ctttgtggca aggacaggtg
     6421 ttatgctaca gcctgtgata acacggagat cctcgaaaat atagaagttg atagggtcat
     6481 tagttgcaac ggtcatacgc ttgcctattt tgctgactgc gatgacgtta tactttcttg
     6541 cagttgcctc cggtatcttc ttgactatat caggcataag ctctgtatta tcaaggtcga
     6601 tataggacac attcagtcgc tcggcgagga cctgacagaa ctgaacatcg gtaacgagct
     6661 tcatgtcaat aatgacatca ccaaaaagtc ttcccttttg ctccttctgt ttggcaagga
     6721 cttcctgaag ctgttcttca gttataagac ctttctgtac cagatactga ccgatagggc
     6781 cgtttctcat atatattact ccaatcctcc gcattgattt agggatccga cgctgcggaa
     6841 acgtctgctg cggtgcaaaa accgcaaaaa aatcatatta
//
LOCUS       DAWEMR010000141         6703 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4593_length_6703_cov_9.506919, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000141 DAWEMR010000000
VERSION     DAWEMR010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4593_length_6703_cov_9.506919"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    940..949
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2723..2732
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1926 a         1324 c         1551 g         1882 t
ORIGIN      
        1 gtcatccatg ttttctgtat aatattctgt ataccctccg aagccatact gcgcaaactt
       61 cttgaagtca gcgggacaag tatcgctttc atagagtttc agccgtcccg aaactgttat
      121 ttcgttgccg cctgatttac ggaaaccacg attgacgctt tcataatcca catactctgt
      181 tccggcacgg acacgtcctc ttcctaaaaa ttttatggcg taatggtgaa cttgtacgcc
      241 atattccatg aatcccggcg tggacggatt aatgtttctg ccttctactg tggcgttgtt
      301 ctttattaca ataccgcctg cttcaaggcc tgaggtgtat tctgaatcat actcttgtgg
      361 tttgtatatc tctttaaaga cgttaccttc gaccttactg ccgtcaactg tcaaaattcc
      421 atataccatt attcctgcaa tagttgaacc tgcattgttg aaatatccgc ctgaagtgag
      481 attttttggt ctgtagtgtg tgtaattggt gcactctccc ttaacaggtc tgtatgtagc
      541 acgaccctct acggtggtat tagtgccgtt tacataaagc gacccctcaa cataaatgcc
      601 gttcatgccc acagttggtc cgtttgcgca agcccagaca gtgctgtagt cattaactat
      661 catattatac tttacgataa tgcaagcgcc gaagctttcg cacgatacat tagtagcatt
      721 ttgttcagtt ccgtcgccga tagtaagatt tgagcagtat attgctgcgt catgtgttac
      781 tgctgaaaac gttccgctgc caatgatcgt gatattactg tcaggagcat aaattccgct
      841 gtagctctca ttgtatttgg ttgcagcttg gcttgaaaac gggctgtcgc caagatcaat
      901 gctgccgaca agctttatgt tcagatcttg ttcgccttcn nnnnnnnnna ccaaatcggc
      961 tgggcctttc gctgttgccg tttatacgtt tgcctatagt cgccatattg tagccaagaa
     1021 gcgtcagggt cttgctttgt atatcgtatt tccagccctg tttgctccat gttttatcag
     1081 atgttacctg ttcgtcgcct atccatatgc catattctgt gaccgcaaat gcgttcaatg
     1141 gtgtaatgga cattaccata catagagtaa gtatgatgct caataaccgt tttttcatct
     1201 tgtttccccc atttcgtcgt cttttgaaag tttaaaatcg aatgtcattt ccaacattgc
     1261 aaacagctta tacatgacct cagtggcgat agctctaata tcaagcgatc cgataaatgc
     1321 aagcactttt gcctgttctt cctccggtac tttatacact cgcattgccg ccgtcagaaa
     1381 acggcgaagc ttgtggtaaa gtgggaaatg gatatcgcat tttcttgcca tatagttgcg
     1441 cattattcct gatgttccga tttccatttc atagaaatcg ctttcggtat agcccggaaa
     1501 attcgagccg aatatctgtt tcagttccgt cgtagtgtga acatagatat actcagccgt
     1561 ttcaggcata gtataagctt caatgtagat ctctcggaga ttttcattca gctcagtgag
     1621 ggttatctgt atggcagttt ccactgcata tgtgtatatc ggcgacatat tatcaccaac
     1681 aatgttcttt gccactccaa attgtccgct gaacatcgtg cccacaagtt ccattagaat
     1741 agcgtctttt gtggagaata agttctgaaa gcttcctctt gcaacgccag ctttttccac
     1801 tatctgcgag atagttgtct gtttataccc ctgttccaga aacagttgga cgcatacagt
     1861 gagtatcttt tttttcgttt cgataccgtc ttttcttgta gaaatgtgta ttagttataa
     1921 catatataac acaaattgtc aagtatcaga aaggggtaac agcgaaattt tacaatgggc
     1981 aacgattttt tgtattattt ttgccttaat gatttaaagc aaaactcttg tattattata
     2041 aaaagtatgg tataatcaaa ttacgaattt tattgtatat ttctaaggag gaaataatta
     2101 tgagtatgta tatcacttgt cttgaccttg agggagttct cgttcctgag atatggatag
     2161 cttttgctga ggctacaggt atccctgagc ttaaaagaac aacaagagat gagcctgact
     2221 atgacaagct tatgaaatac cgtatcaaca ttcttaagga gcatggtctt ggtcttaagg
     2281 aaattcagga tactatcgca actatcgacc caatgccagg cgcaaaggag ttccttgacg
     2341 agctgagaag cttcacacag gtaataatca tcagcgatac tttctcacag tttgcaggtc
     2401 ctcttatgaa gaagcttggt tatcctacta tcttctgcaa cactcttgaa gttgctgaca
     2461 acggcgagat cacaggcttc aagatgagat gtgaaaagtc aaagctcaca actgtaaagg
     2521 ctctccagtc tatcggcttt gaaaccatcg caagcggtga cagtcataac gacctcggta
     2581 tgatacaggc aagcaaggca ggcttccttt tcagaagcac agacagcata aaggctgaat
     2641 atcctgagct tcctgcttat gaaacatatg atgagcttat ggctgcaata aaggctgcta
     2701 tggactaata gtctttaata aannnnnnnn nnatatcact tataatggca gcggctttgt
     2761 cgctgtctgc ggcagcctgc ggtcaaaatt ctgacagcag cgtggctgat aagtcaagct
     2821 caaaggctga tgaaaagcct gccgtggaaa gctgtaagat agctgatgat aagttcgata
     2881 catatgtgtc gaacacttac gttgccacgg gaaacaattt tgtggtaaat aaagctaacg
     2941 aggtaacata cagagcctat tttccactgg aggaatacgg agagcttgaa tacgctttct
     3001 atttttcaaa tactgtggat tctacataca acgctgacgg aaagcaggct tttgcaggca
     3061 aagagggcgg agagtatgag atttcctcgg cttatgtctg tgacggtgga acaggtccgg
     3121 atgacgagat aacctcacgc actgaggtaa catttgacgg tgcaggctca aagaaagtcg
     3181 cacctgcgga aactttttgg agcgaccctg ttaccctgaa tatccctgag gatcattatc
     3241 tcgtgtggga atggacggtg acgggaagag atatcccatg caacaagatg tcaaacctca
     3301 cgtctaccac atcatcgaaa aatggcagcg atttcactta ttgtgacgat gtgccactgc
     3361 cgcttcttat aggagcaaag cgtgatgtga aatatcgtgt tacggctatt ggcgattcta
     3421 tcacacaggg ctgtatgaca gacttcatgg cgtatgaatt ctgggcggca cgcattgcaa
     3481 aagagcttgg ttctgattat gctttctgga actgcggtct tggctgggcg agagcgagcg
     3541 attgctcaca gaaaggcaat tggctcggaa gagcgctcaa cagcgacatc atgatagttg
     3601 cgttcggcac aaatgacatt gtttcgggcg aatacggcgg cgacggcgga aacaatgccg
     3661 acgaggtgaa tggctatata agcactatcc ttgaacaatc tcaggcggca ggctgtaaga
     3721 ctatcttgtt caatgctcct ccacaggatt atgacgagga acacgaagcg gtgagaacag
     3781 cacttaacga tactgaaaag cagacggctg agaaatacgg cgcagagtat tttgactttg
     3841 cggcacagct ttcaacacct gaaaacccag caggtgcgct ctatggcgga cacccgaacg
     3901 gcgagggtgg cgaggccgtc tgctaggcat tcatgaaaca attcgcagag cttttaggca
     3961 aataatattt gtttttatac ggcgttgtgt cttttgacac ttcgccgtat ttttttgcaa
     4021 aacaatacca aacataatct gcgatatgta acgtatatta tatatagtat cttcgctttt
     4081 gccgtgaata ttttatcata tgaaaagttt atacttctga cataagtttc ccttgacaaa
     4141 cgtggttagt ttatgctaat atgaatatgg ttagtaaatg ctaacctaaa ttaaagaaaa
     4201 ggaggagtta agaaaagtat gagcgttata atagtaggcg gaaatgaccg tatggcaaca
     4261 cgctataagg atatctgcaa atcatatgat tgcaaggcta aggtgttcat caaatatcct
     4321 acaggttttg ataagaagat aggcagtcct gatctggcag tggtgtttac tgatacagtg
     4381 tcacataaaa tgctcaatgg tcttaaccaa agagcaagca aacttgattt tcccgtggct
     4441 atatgccatt catcaagctg ttcggctttg aagaacgtgc ttgaaaaata ttgtacaaaa
     4501 ggcgttgtaa acgcatagat ctatatgtat aggttaggga tttctaacca aaggagggac
     4561 tatgtcacag tcagagctga gaaattttga aaaaagcatg gcttatcatc ttgcccctgc
     4621 aatgcttgga ataaagcctg cctgtcttat gtcaatggaa aagaacaagg acgggcttga
     4681 agagaatgtc gagcttttta acagcagggc atatacaaag ggcatgaagc taacagtgct
     4741 ttgcgagtgc aaaagcagaa agctgctttt tctctataac gaaaagcttt tatctgacag
     4801 acttgcagac aacagagtaa gaaagcttct tgaaagcttt ggctatgaca gccgtatgac
     4861 ccttgaacag cagcttgaaa ggctgtctgg aagaatatcc gcaagtgagg attttcctca
     4921 tgagataggt ctgtttctag gctatcctgt tgatgatgtt acagggttca tacagaacaa
     4981 gggaagaaac tatcttcttt gcggatattg gaaagtctac tctgacgaaa acagagcaag
     5041 acgtattttc agcaattatg acaagtgcag aaattatctg tgtaataaac ttgctcaggg
     5101 cgaaagcatt taccaagccc tgaaaatttc ttaggaggta atcataatga aaacagcagt
     5161 aatttattgg agcggtacag gcaacacaga ggctatggca aaggcagttg cagagggtgc
     5221 aggcgcagag tgcttcgcag tttctgagtt cagcggcaat gttgaggatt atgacgcaat
     5281 agctcttgga tgccctgcaa tgggtgcaga ggagcttgag gacagtgagt tcgagccatt
     5341 tttcgcaggt atcgagggca aactctcagg caagaaggta gcactcttcg gctcgtacgg
     5401 ctggggtgac ggcgagtgga tgcgtcagtg gcaggacaga gttacgggcg acggtgcaac
     5461 acttgtaaat ggcgagggac ttattgtaaa cgaaactcct gacgacgacg ctctcgcgag
     5521 ctgcaaggct ttgggcgagg ctcttgctaa gtaagcatat atctctaatg taataatgta
     5581 aactgcaaac gcaggcggaa aaaggcagtt ttgcttttct gtctgcgttt gtgcagtaat
     5641 tgagttatta aaaactaact aaagttatac taaactaact tatgattttg tgcaagctta
     5701 caaaattgaa aaaacggcac atttagtctt gacaaattcg gttagccgtg ctaaactata
     5761 ttacgggtta gaaaatgcta acccttttaa taagcctgaa agttagataa atctaacttt
     5821 tgatactgac aaaagaacag gagggtctga atgatgccgc tgacatttgc aaacgtaggg
     5881 gaagaaaaca tgataaagaa agtaggcgga aaacctgaaa caagaaagtt tcttgaaaat
     5941 ctgggttttg tcgcaggtgg aacggtaacg gtagtaagca ctatcggagg aaacgttatc
     6001 gttaaggtca aagaatcaag agtagctgtt tcaaaggaaa tggctgcgaa gataatggta
     6061 taggaaacat ttggcggtca agccatgata aatatgtttg aagatatttt gtatagttag
     6121 gaggatcagg ttatggcaac attgaaagaa gctaagatcg gttcaacagt taccgtgaag
     6181 aagctcaacg gcactggtgc tgtaaagaga aggatcatgg atatgggcat cacaaagggt
     6241 gttgaagtcc atgtaagaaa ggttgcgcct ttgggtgacc ctattgaagt aacggtcaga
     6301 ggctatgagc tttcgctcag gaaggctgac gctgagatga tcgaaacggt ctgatacata
     6361 gttcaggcaa acagcaaaaa atttcatcgg cagagctgat gtgtacttag aagatgagga
     6421 tatatcagag tgccgaaatt ctgaaaggat caaattgtat ggagatcaaa attgctctcg
     6481 caggtaaccc taactgcggt aaaacaactc ttttcaacgc tcttacaggt tcaaaccagt
     6541 tcgtaggtaa ctggccgggc gtaacagttg aaaagaaaga gggtaagctc aagggtcata
     6601 aggacgtagt tatcatggac cttcctggta tctattctct ttcaccttac acacttgagg
     6661 aagtagttgc aagaaactat cttataagcg agcgtccgga cgc
//
LOCUS       DAWEMR010000142         6608 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4671_length_6608_cov_12.559591, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000142 DAWEMR010000000
VERSION     DAWEMR010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4671_length_6608_cov_12.559591"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1950 a         1354 c         1722 g         1582 t
ORIGIN      
        1 tcagggggcg ggcattttct gtgatatgaa aagtgagaat attatgaaaa agcatacaca
       61 aaaaaacagg caaataataa tgctgtcagc agcggcgttt tttatcgcag cggctttttt
      121 gtgtatgcgt ttttataata taagcagtta taagacacct gaaaatccgt tttcagcact
      181 gtctgaaagc cttgcggagg aggacggaca gggcggagag ggctatgaaa caagctctca
      241 gtcaaaggac gaaagctcag atatggacag ctcacaggca gatgacagtg ataacactga
      301 tagtagcgac agcaatacag atacaacaga aagctctccg caggctgaca aaagcgacgg
      361 aaattccccg acagtaaatg gcgggaacac gaatgttacc ataaatgccc aaggtgaaaa
      421 taagtctgac gaggtaaaga aaaagcctga cagcaagacc gaaactgtca caaagaacac
      481 cgacacagaa tatttcacca cctctataaa agagggcgag cgtgtaaaaa agccacttta
      541 taagttcaca ataacacata aaaacaaggc tctcacagtg caaaaagttc aggtcactgt
      601 gaacgacaac gttgtaaatc agttttcggg cagcgttacc cttcacgcag gaaaaaacac
      661 tatccgtgtt gcctgcactt atacagacaa aagcggtcag gtgaaaaggg cttataagga
      721 ctatacagtt tattttgata aaaaggagat aacctttgac accgaccttg ctgataagga
      781 tactgacaca gaaagcataa cattttcagc cacagctttt tacggcagtg ataagatacc
      841 tttgacagta aaagtcaacg gtgaaaagct ttccggtggt gaaactttta ccgcacagct
      901 ttctgagggc gagaatacta taaccctctc tgcggaaaag ggcggctcaa aggctgaaaa
      961 aagctttaag ataaaataca ctcctgcgga gttttccatc gataccgacc tttacgaccg
     1021 cactgtaaat gacgccgatt ttagcttcta tgcgagaatg aaaggtcaaa gcggcagtgc
     1081 aaagctaagc gttattctaa acggcaaaaa gctttcgggc agagataatt atacctgcac
     1141 cctccaaagc ggtgataacc gtataaggct ttatgcaaaa gacggtgaca aaaaaataga
     1201 cagatatttc actgtcacct atgtacccat tgctgacgat accacaagac ctgagataac
     1261 gtatataaac gtcaccaacg gacagacagt gaaaggctcg ggcttcaccc tcagatttaa
     1321 cgcacaggac tataagggcg gaaggatata tgccgacaag accgaggtct ggctaaatgg
     1381 gctttctgtt gagtgtatcg cacaggacag gaactcagcc tattatctcc agctttcggg
     1441 cggggcaaat cttcttgaaa taagggtgta tgacccagag ggcagatatg cggatcatgc
     1501 ctataccata aattctgtca gcgcacaaaa gggcgagcag acaggcgtta taacaatgag
     1561 ctttgacgct gataccatag ggcttggaca gcttgccgca ggctctgagg tggcaatata
     1621 cgagggggac acaggcgttg acgttataga gcgttttctc cagcaaaacg gcttcacagg
     1681 tgactttaca ggcaagggcg atcagagata tctaagccga atacacaaaa gcggtgcgtt
     1741 ttcaggtgga gcagtaaaca gtgagcttgc ggagcttgta aaaaatgatg gcattgcaaa
     1801 cagtggcaca cagtatgccg acagccttgg agagtttgac tacacctacg gctcaggctg
     1861 ggtgtacact gtaaacggca atatgcctgc atacggcatg ggcaaggtga actttaccga
     1921 cggcgacact gttaggcttg cgttcacagt tgcctacgga agagatataa caggttcaca
     1981 ggatagctat gacaaaacat ggtaggagga tattgttgaa aacgagaggt tttcttggca
     2041 gatcagtggc ggtgtttgca ggggcagtaa tgcttctgac aaatgttcct gcggtgaatg
     2101 cagataacgc agataaaagg gacaggataa ctgaaagcgc agaaaaggtc tgtcagtggc
     2161 agcgtgacaa aatgggcatt tctcaggacg agagcatttt ttcggggaat tttctgcaaa
     2221 acgcaggcat aggaagctcc gattggcttg ccataggcat ttcacgtttt ggttttgagg
     2281 aggactatga ggcttatctc actgcgcttt ctcagcgtgt gaaagctctt tcagataccg
     2341 acaacgccac tgagtggcaa aggtgtgcta taacagcctc cgcaatggga ggcgatcctg
     2401 ccgaccttgg gggcatagac cttgtaaaag gcggtgttta cggcagagat gaaaataatt
     2461 ccgtaggcaa acagggcctt aacggctgga tatttgctct tttgaccctt gatacaatgg
     2521 gctacaagac cccagagggt gcagagtttg acagagagcg tatacttgac gagatagttt
     2581 cttcgcagaa taccgatggc ggcttttcgc tgtcaaaagg tgaaagcgat atcgacatca
     2641 ccgcaatggc tttgcaggcg atcgctccat attacaatga tttttcccgt gacgatgtta
     2701 gaaaaagcgt tgacaaagcg gtggagtatc taagcggcaa gcaggacagc tcaggtacgt
     2761 tcggaagtgc agaagctgat tcacaggtag taatagcact ctgttcactt ggtatagacc
     2821 ccgaagctga cagccgtttt gtgaaaagtt ccgacctttt gaccgccctg ctttcctatc
     2881 agaacagcga cggtggattt tcacatgaaa agggcggcga ttctgacgag cttgccacag
     2941 ggcaggcgct ttgcgcactt gcggcacaaa agcggtttga acttacgatg cgacgtattt
     3001 acgatatgag agaggagctt tcagttctac agcgtgaaaa gcttgacggc ataaacggca
     3061 ggctttcgga tatctcagac gaggagagcg cagaaaaagc cttgaagctt ttcaatgacc
     3121 ttgattgtga cgaacgcaca tatgtgagat acggtgcaga gcttgaaaat gcggcagaaa
     3181 aatacagcct ggccttgagc gacagggctt tcacagtgga gcttgcacag acagatcatg
     3241 gaaacggctg tgtatacagt atcgagaaaa cggaaatata taagggcaag aaaggcttta
     3301 caaaaagcga caggaacaag cttgaagcct tgagaaaaaa cggcgttact tcgggggact
     3361 gcactgttac agctgtgctt ttagtccatg caaaggctga cgagagcctt tccgacaggg
     3421 ataagataat atcagagctt gaagaaatga acgccaaggc aaacgagctt tacagtgaga
     3481 tatcagacct taacagtatc ataagccgtg agctttatcc tgttgatagt gttggaaaag
     3541 acaaaaagga gcttttggaa aagaccgcag agaggataaa aaagcttcct gaaagcgagc
     3601 ggaaaaaggt cacttctgct gacgagataa taaaagaggc ggaggataaa aacgtgacag
     3661 tgtatgtgat atctgcggca gcggtgctgt gtgcagtggg agttttcaca gtggtaagaa
     3721 agaagggtaa aaaatgcgtg agataacaga tgaagtgaaa aaggtcataa tcggcaagga
     3781 cgaagtggtg gatatggtgt tcgccgcaat tttggcgcag ggtcatgtgc ttatggagga
     3841 cgtcccgggc gtgggaaaaa ctactcttgc ggtggcgttt tcaaaggctt tggggcttga
     3901 aacaaagagg atacagttca cccctgatac tgtggcttcc gacatcacag gctatacggc
     3961 ttttgacaga caggcacagg gctttacttt tcacagcggt gcggcaatga cgaatatact
     4021 tcttgcagac gaaataaaca gaacctcagg caagacgcaa tctgcccttt tggaggtcat
     4081 ggaggagcgg acctgcacag ttgacggcag aacatatcct ctgcctgagc cttttgcagt
     4141 gatagcaaca caaaatccta caggcacagt tggcacggcg gcactgcctg tttcacagct
     4201 tgacagattt atgataaagc tttcaatggg ctatcctgac aaggcggcaa tgcgaagcat
     4261 aatgtcagac cgaagcagtt cagatccact ggtagatgtg aaagccgtct gcaccgctga
     4321 aaagcttata gaaatgcaga aaaaggcaaa ggctgttttt gttgacgaca gcgtttatga
     4381 ctacatctca gacctttgcg acgccacaag gcataatgaa aatatttctc tgggcgtatc
     4441 cccaagaggt gcgttggcac tttgcaggat agcaaaggcg tgggcgtgct ataagggcag
     4501 agaatatgtc gttccccagg acgtaagaga tttttttgcc catgtttgca gccacaggat
     4561 atcactttct gcaaagggca ggctatcagg acttgcggca gaaagtgtgc ttgacgagat
     4621 acttaaaagc gtaaaatgtc ctgagaatga ggaaaaacgc agatgaagag gctaggcttc
     4681 tgtctttgca cagctttggg catactgctt tttgtgctga aaaagcccat tggcatatcg
     4741 gtgcttgggt tgtgcgcagt gctttttgta ttgtcagtat gtataaatat attctcgaag
     4801 aaaaagctgt cagcaacact ggtaagcgac ggcacaggct tgaaaaataa ggacatgagc
     4861 ctaaaattta tcttcacagg aacttgcggc gaatacagcg gtaaactgcg gtttagaaat
     4921 atgctcacag ggcagtgcga tgatataaag gtgcatttta tcatcagcag agagcctgct
     4981 caggtggata tcactgtaaa aagcgacacc tgcggaaaga taaaagcaga gtttctgaca
     5041 cttagagcct ttgactttac agggctaaca tggaaaaagg cgcagtgcga aataaagggc
     5101 agtgtgaaca taatgcctga tgccgcagag atagatgaga gcgaggaagc aaagctcaag
     5161 gaagcccttg aaagcgactt tttcagcaat gaaaacgagg gctttgacca cagcgagcct
     5221 attggaataa gagaatatcg tcagggggac agctataaat cggtgcattg gaagctgtct
     5281 ttcagacttg gtgatatcta cgtccgtgaa tatggcgagc ctgttactga cagcctttgt
     5341 atctacgttg aaacaggcgg aaatgtcccc gcagcggatt atgaccgcat tgccgcaaag
     5401 gcactgagcc tttcaatgta tctttcagac ggcggtatag ggcattacat ggcgttttac
     5461 agcttttctc agcagagaaa agttttcgag catatcgaca gcgcagacag tttcactgtc
     5521 gctttggaca gccttctttc ttcacagcct gagcagggaa aagagcggct gacggagttt
     5581 tcagagctgt ttgaaagaag cggcataagc cgtgttatcg agataaaata gtaaaagagg
     5641 tgacaatttt gacagcaagt atttgctatg tggaaaagca cttttcaaag ggtgtttata
     5701 tcatgcagag cctttttggt gcagtgacaa tgctcctgct gtcattgtcg cttggtatgg
     5761 gctggcgagc ctttgtcctg ataggtgtgt gcatattgac agcccttgca ggggtatcaa
     5821 aaaagacttt gcctatacca tttttcaccg cactattgtc ggcggtgata ttccacaaga
     5881 agtttttaac aagccttgca gggcttttga actgcgtctt gatcgcccac ggtaaaagcg
     5941 caggagtagt ggaaatgctt gtggaaaaaa gctcagaccc ttggcttgct ctttgcgtgg
     6001 gtgcagtgat agtttccaca gtgcagagca ttttcagccg tcttgacagc agagttttta
     6061 cagtggcaat gcttgcgctg tttttgtgcg caggtatctt tattgatatc cctctgtact
     6121 atgccactgc agaagttgcg ttaggtatcc ttgcattgac gttttccggc agcataaaag
     6181 gcgatttgaa gccctttgtg cttatactta cagcattttg cgcagtgctt atagcctgcc
     6241 tgacagggct ttttgataag ctttccaact ttgcatttgc cgatcgcttt aatacgaaaa
     6301 gtgagtatga gatcataatg gagcacccac agccattgta tcttctggag agccgttttg
     6361 agaaagaaac taacggcgtg tggggggagc ttagcggtga ggagaaatat gcctactcag
     6421 acgagttcta ttggcttgat aagcttgatt tttcccctgc ttttcagact tcttcccttg
     6481 acaatgccct tggtatatcc gacagcggca atgttacagt aaacatcaaa aatggcggaa
     6541 agccccttta tacctatggt atgaccgccg cagagaacgc tttttccgac ccaaagataa
     6601 taggcgga
//
LOCUS       DAWEMR010000143         6522 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4751_length_6522_cov_14.224679, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000143 DAWEMR010000000
VERSION     DAWEMR010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4751_length_6522_cov_14.224679"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1565 a         1435 c         1556 g         1966 t
ORIGIN      
        1 gtggggtcta ggggcaaagc ccctagcgga gtatgaggca gagcctcatt cggcgatagc
       61 cgactaggtg cagattgtag tattaaaagc atatagaaat aagatatcag acaaaagaaa
      121 tatcggagta acacaatctg caccgctatt ctatcccaaa gcaaaacgta tatccgcaaa
      181 gagcggagcg attttgcggc ggcagaaact tgcccgaata cctgtagggg agcagcgcaa
      241 tttctgcttt gcagaaatac gacgtcccgt ttcgaggagt tgcggggggt tgaatttgta
      301 caaaccatgt aggcgaataa aggcgggcga acgctgttcg cccctaccgc catttgtggg
      361 gttattgggg tttgtgcaat aatttttagt ggttatgttt cgcaatattg tgtatatgtg
      421 ccatatggca ttttgaaaca acgtgccgaa ctttgtatgg ttaccgcaag aatggacagg
      481 caaacaggtg cgggctgtcg agggcgccag cccctacatg gtttgtaaag tacataacaa
      541 agtgcggata acgcaaataa aaaatgcgaa acgaaaaccg ttccgcattg gtatcatatc
      601 aaattatttt tccaccgcat atcaccatgt caggtgatac cattacatca agggggtcgc
      661 cgctgaatag ggtgaggtct gcgtcaagcc ctgtctttac agagcctacc cggtcggata
      721 tgcctgctat ctctgccgct gtgcaggtga tagcttcaat gcccttttca tagtcaagac
      781 cgttttttac tgcaatagcc gctgacaagg gcagatattc tatcggcact tctgcgtggt
      841 cggtgcagat agctaccttt atgccgtgag aagaaagtat gcctgcgttt gctgaggtaa
      901 gatttgccat ttcaggcttg cacctgtcgc atattacagg acctatcaca cagcttgcat
      961 cttcctctgc aagctcgtca gctatgatat gaccgtcagt gcagtgtata agcacatagt
     1021 caagctcaaa ttctttcgct atccttatgg cggtgaaaat atcgtccgcc ctgtgacagt
     1081 gaaaatgcgc cttgacttcg cctctgagaa cagggagcag agcctccgac ttcatgtcaa
     1141 actcaggcat atcctcctcg cttcccattg cacgctcgac cgcttcaccg tatttctgcg
     1201 cctttataag tgcttctctt attatgcctg ccactgccat tcgggtgtag ggggcgtcct
     1261 ctttgtcaga ataggtgagt ttagggtttt cacccattga gaatttcatt ccaactgtgc
     1321 ctacaagcat tttatctatt cgcttgccat aggtttttac tgccgcaata ctgcctgcga
     1381 cagggtttgc tgagcctggg gatacaagac agcatgttac gcctgctttt cttgcaagct
     1441 caaaggaata gtcgatgggg tttatgccgt caacaatgcg gatatgtgct gtgcatggtt
     1501 cagattcctc gttgaaatcc tcgccctcca tgcctacacc gcttgtggat agtccaagat
     1561 gtgtgtgaat gtcgataaaa ccagggtaaa ctgttctgcc gttgccgttt atgtcctcgg
     1621 ctgtgacagc ttcgggagag ccgctttcta cagcggaaat aatgccgtct gttatctcaa
     1681 cccagccgtt ttcaataaca cgtctgtcgc tgtccatggt ctttatttta acgttgtaaa
     1741 ttttcatatg cagttttcac cattcttatt tgcctgttga gccaaagccg cctgcgccac
     1801 gctctgtatc ggaaagctct gttacttcct ctacttcggg agtgaaaaca ggggttatca
     1861 caagctgtgc gatcctgtcg ccactgttta tggtgtagtc cttgtctgaa ctgttgtgga
     1921 gagcgcagat tatctcacct ctgtagtccc aatcaacaac gccaacgcag tttgcagggg
     1981 caatgccgaa ctttgatgcg agtgagcttc ttgcatagat aagaagaaca cagtttttct
     2041 caccctgaac ctcggctgat atgcctgtgt gtattttctc ggtctgacct gcttttatgg
     2101 ttacaggtgc gtctgtgcag gcacaaaggt catagcctgc gctaaattct gtttgtcttg
     2161 cagggataac tgcattttcc ttaagctttt ttataagtaa tttcatttta taaaactcct
     2221 ctgtagtata ggccattggt ggcgttttcg ggctttgcga ccctttcgcc atggtaagcc
     2281 tttattattt cgtttatcct gtgggcgttc tcctcataga agtgaagctc catatagtcg
     2341 gcattgtcaa aatcgtcgag cttgtctgca agatacaaaa tatcggaatt tagtatctct
     2401 gcgcaatctc tgccctttgg acatctcacc ggaaactctc tgcctgtgcg gtcaaaaagc
     2461 ttgcccttgc agtttttaca gcccatatcg tttcttatag gacagtttgc agtgagcata
     2521 aggggaagtc tgccgtatgc cacaaagcct atctcaatgg gcttctttaa cttttctatc
     2581 tgcgacgctt taagctcaaa ggacactgtg cagtcttttg caccaagttt ttttacctcg
     2641 tctaatgcgg ctgagttgca gagattgaat gaatagcctg cgtgaagctc aaagccaagg
     2701 tcattcgcaa taaggcagtg gccgatattc atggcgagca tttttgttat gcctgcttct
     2761 tttgcggctg aaagcttttc acatagggct ttctcgtcga atgtgaagcg tggcattatt
     2821 atcataagct tgcttttgtc tgacacaagc tttgccgctt ccaaagctat ctctatcggc
     2881 acgcccacaa gctctgcttt gtcaaggtca acaccctcaa gctgagagag ttttgagagc
     2941 attatgcgga tctttttgtg ttcataggct tttggcaggg taggaagatc ggcaagtgct
     3001 ttgtcggtaa aatcggcggt atgggtgaaa aagtctatac gcttctggtc aagcttttct
     3061 accgcctgcc gtcttaggtc attaagtgcg gaagctggat aggcaaggtc tttgcctatg
     3121 gcggcttcaa gtcctccgaa ctcatatatg gtgtctccaa gcttggagag ctgttttttt
     3181 acatagtcgc tgtcgcaggc ctttttcaag gctttttgtg gtatatcgcc gtaaacggtc
     3241 acggtgtttc cctcgctgtc acgaagagaa agctccgccg gagaactttc ctgaagcttt
     3301 atggtgaaaa aggcttttgt gcgcttttca catctgcggt aaagctcatg aagctcggga
     3361 agaattttgg cggcggcgga aacgtcctct ttctgccttg tgccgaacat ttctctgcct
     3421 aatctgccat tgtaatagcc gtctgtaaag cccccgcggg aaaagaccgc ttcaagggct
     3481 ttcaggtcat aaggcttgtt ttcgagagaa tttttacagc agttcactgc cgccgcaaca
     3541 tattcagggc gcttcattct gccctctatt ttaagagagg aaactccgtc cttttcgagc
     3601 ctttggagct tatcaacata ggacatatct ttcagagaga gggcgtaacg ctcctgcccc
     3661 tttgttatct tatctccctg agcaggcagt ctgcaagcct gagcacaaag tcccctgttg
     3721 gcacttctcg aacctatcaa cgcactcata tagcactgac ctgacacgga catacaaagt
     3781 gcgccgtgga caaagacctc tgtttcgatg ccgagggcgg tgaggtcttt tatggtatgc
     3841 tcgggaagct ctcttgacag gacggctctt gaaaaaccga ggtttttagc cattttcaca
     3901 ccgctttcgc tgtggatggt catttgggtg gaggcgtgat attcaaggtc ggggcaacat
     3961 tcacggcata ttttcacaag ggtcaggtct tgggtgataa gtccgtcaac gccaatttct
     4021 gcggcaaatt tcacggcttt tttgcactct tcgagctgtt cgtcaaagac aacagtgttt
     4081 atcgcctgat agacctttac acctcgcagg tgacagagcc ttacggcttc ttttagctgt
     4141 tcatctgtga aattggcggc attttgtctg gcggaaaatt tctctccccc gagataaact
     4201 gcgtcacagc cgcttctaag ggcggcttcg agagtttcaa aagaaccgca gggggcaagt
     4261 atctcagctt tgtgagccat taatggcttc ctccctcggg tctgtaattc actgtgccga
     4321 caaaattgcc gttgttaggc tgattagggt ggacaggtct ttcaatggcg ttctttatct
     4381 cctgcgaaag aagttcgttt gcgtttataa cttcgtcctc aggctctctt tttacaactg
     4441 tgcgtgcttc gagctgttct tcaaggtcat ggacttttgc ctgaagctct cttatttctt
     4501 tctgagcttc gtcagcctga cttcttgctc tgcctgcgtc gtcaacatag tcctttatct
     4561 gggtgcggat attctcgata ttctgatttg ccttgctaag ctcgtcaaga caatcaagtg
     4621 ctgtcagcac tgcggcgtcc tgaacggaaa ggctcgggat agctttcatc atgcctttta
     4681 tctttgaatt tatcaccagc gcaagtttat tggtatactc ctgcgactct tcggaaagca
     4741 gattgtatgt acggttataa atatttactt taagcttgtt tttcatagct tttgacctcc
     4801 ctttattttc tttgctgtga acttgtatac ttaactgtat tatacactac tttttgagaa
     4861 aaataaagag ggtaaaaaga agttttttgt aaatacgccc tttgcggtgg tttttgcttg
     4921 acatttttac aaatatgata tataattaat catagatttt ctataggatt tatgaaagga
     4981 tctgacgctg atgaaaatat ccacaaaggg aagatatgca ctgaggctta tgcttgacct
     5041 tgctttacat gagcagagcg gatatgtgcc tatcaaagcg atcgctgaaa gacaagacat
     5101 cagcgacaag tatcttgaac agataatatc cattcttgca agagcgggct ttgtaaagag
     5161 cgttcgtggt gcaggtggcg gatacaagct ggcaaaaacg cctgcggaat acactgtggg
     5221 aatgatactt cggctcacag agggaagtct tgctcctgtg gcttgtctgg agggggaaga
     5281 aaatacctgt ccacgtcagg atagctgtgc aacgctgacg gtatggaaaa agctttacgg
     5341 tgctataaat ggtgttgttg acggcattac cttgcaggac cttgaaaatc aggaaaatgc
     5401 aaggatagat gattacgtta tttgatattt tgcaggggcg ggttttgctc gcccgttttt
     5461 tatgcgtttt ttgtgcgatt ttaagctgtc tgtttgcaga atggagtggt cattgcctat
     5521 gaccgctctt tttttacgcc tttttatctt ctacctatag cacaaaagaa agcctatatg
     5581 acagcttggt tttgtgcata attgattttg tcatgttatt gtaaaattat tgtgggatat
     5641 ggtattgctt tgatgcacaa atgtgatgtt cggataggac ttttggattg tgcaaaagat
     5701 acaatctgcg gtggaaaaag tgcgaaactg aggcatttgc aaatcatagt ctgggggtat
     5761 aaaataagga tagaaattaa attggagtga taataatgag tacaacagtt caggtaaaaa
     5821 attcaacaaa gcatattcag cttatatgcg tgacaggtct ttttgcggca atgatatatg
     5881 ttcttacggc atggctgcac attcctacgg gtgcaggcta cacacacgca ggtgacggac
     5941 ttatttatct tgcggcttca atgcttccta caccttatgc aatggcggca ggcgctatcg
     6001 gagcaggtct tgcagacgga ctttcgggat ttgtggtatg gctccctgga actatcgtta
     6061 taaaggcgat aactgccctt tgcttttcaa gaaagacaga gaaaataatc tgcgtgagaa
     6121 atcttctcgg cattatccct gcacttgtta tatgcgtggt atgctacagt ctttatgagg
     6181 gaatttttat ggcaggcggt ttttcaaaga gcgcaataat ttcggctttc ggtcagactc
     6241 ctgcttactg catacaggtg cttgcaagct cgatacttta tatagtgctt ggtcttggtt
     6301 ttgacaaggc tgggcttaaa aagaagtttt cacatttgtt tggttaaagt agacgtcata
     6361 gcggtgaaac aaagcgggcg acctcaggtc gcccctacac attatttgca aaagaaaaaa
     6421 aggctcaaac aagcgtgacg cctcaatttc ctacaaacga cgtaaatacg ctgtttgtat
     6481 gtaggggcga acagtgttcg cccgttgatt tgacattttt gc
//
LOCUS       DAWEMR010000144         6499 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4769_length_6499_cov_9.744569, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000144 DAWEMR010000000
VERSION     DAWEMR010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4769_length_6499_cov_9.744569"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2007 a         1145 c         1458 g         1889 t
ORIGIN      
        1 tttattcatt cttgctattg tgctttcagc gtatactcta gggaatctac tcattgtgat
       61 cttacctcga tatttttata ctttacttga ttatacatta gctgataata ttagtcaagg
      121 ggaggggcag aaaatcaatt ctttttttgt caccccatag gtaggttaat cattatctga
      181 cagcaatgaa tactcacaca aagctgtaat gccggctttt ttctctaagt gaatatcatc
      241 aggaaacgca tatcttctga taacagggtc tctcaaaata tgctgtatct gtttccaaag
      301 ctgctttaca tcaggcggaa ttgtaattgc tctcgacttg ccttttgtgt caaggatagt
      361 cacattcagg tattctattt catcaaaaca tctgcttgcc gatgtttttg atacgcccgg
      421 tttttggcga ctattgacgg cgaaaatagt atcatattaa agtatctggt ttaattcgat
      481 ttttttatta ggtcttgtta tggtgaaaaa ttttacgcca ttttctttgc tttatttact
      541 gcgaaataca tttagcacgc cacaataaca ttacgatttt tacacgaata ttaaaattat
      601 gatataattt tcagccatat agtgatacga tatagttgca aacaaaacta gaatcagaaa
      661 ggacggttaa aaatatgaga agatttaatg gctatatgca cggcatcgat atcggaggct
      721 ggctgtcaca atgcgattac agtaaggaac atcttgataa ttttattact gaggaggaca
      781 tcaagcgtat aaagggctgg ggctgcgacc acgttagagt gcctgtggat tataatatct
      841 ttcaggacga aaacggcttt atagagagcg gcttcgacta tgttcagaag tgtatcgatt
      901 ggtgcggaaa taacggtatc aatatgattc ttgacctcca taagacaatg ggctttttct
      961 ttgacaaggc tcaggctgaa agcggattct ttgataacgc tgagcttcag cagaaattct
     1021 acgacctttg ggaagagttt gcaaagcgtt tcagcaaata ttctgacagg gtctcttttg
     1081 aacttctcaa tgaggtaact gatcctaagt tcagcaaaaa gtggaatgct atcgttgaaa
     1141 atgccataaa gcttattaga aaatatagtg ctgatataaa tattattgtg ggcagctact
     1201 ggaacaactc tattgattca atgaaagacc ttgataagcc ttatgacgag catatagttt
     1261 atactttcca ttgctatgac ccatttcttt tcactcatca ggctgcctat tgggtagatg
     1321 agatgccgag agatttcagg atcgattatc ctggcagcgt ctcaaaatac agaactgaga
     1381 taaagcgtct tggtcttgaa tatatgcaga catacgagga ggtcaagaca gagaaattta
     1441 cgcctgatta ttttatggga agatttgctg aggctgtaag ggtcgcagag gaaagaaatg
     1501 ttcctatgta ttgtggcgaa tacggtgtta taaatcttgc atctgctgaa aatactctta
     1561 actggtacaa ggatataagc agtgcctttg agaaatgttc tatcggaaga gcggcttggt
     1621 catacaaggg cgttgactat ggttttattg acggacatct tgacagcgtt cttgatgaac
     1681 tggtaaagta tctttaatag atcatttctt aatataactc catttttgga caagctcggt
     1741 cacgtttgcc gagcttgttt tttatgctta gtttcataaa ttgatgtttg ctcataaaat
     1801 tctatttacg ataattattt gatataaata actgaatatt gatataataa ttttaaaaca
     1861 aagcgtataa tataaacata ataattcata gtcactttct tataataact tccgaataaa
     1921 cagacctgct ttaatatcaa gtgggtctgt tttcatatat ttataatctt tcataaattc
     1981 gatattaaac acttttatga tatttatgtt acgtttttga tataaacaaa acgtttacat
     2041 agttataata tatacagaaa caacaaaata aattgctttt agcgaaaaat tttattgaaa
     2101 aataggagga aaaggttatg aagatcaaaa aatttgtagc aggtctttca gcagcggttc
     2161 tcgcagcaac aatgatgtca gtttctgttt cggcagcaaa gcttggcgat gttgttcacc
     2221 cgtcagagga ggactcaaaa atgaatgact catattactc tatcggagca atgggctttt
     2281 atatgggtca ggactggacc tggaatcagg gtgactgggt aggcatcgat gatgctggca
     2341 agataactgt tgaatataag ataagcaaag ttatcaccga caagacaatg tcaggcaagg
     2401 gaactcttgg agatatgggc gttatggtgg caaatctgcc tgatggtatt tatcctgtgg
     2461 acgttaagat atctgacgca aagttcgttg ccgaggacgg cacaacaaca gtttttgatt
     2521 cgcttaattc tatcacttca atggacgagg acgcagaggg tggaataaga atacatatca
     2581 gaccaactga cgaggtcaat gaggaaacag gcgaggtaac aaagaaagct tgccctgagg
     2641 tcgcaggctg ggagaaggaa ggcgctttca acggcggcac actttcaatg acgcttgatt
     2701 tcggtgttgc atctgccgag ggcggagagt caaagacaga tacttcaaag gcagattcaa
     2761 agtctgatac ttcaaactca aaggctgata cgtcaaagaa agacgtttct tcatctaagg
     2821 cagatgcagg caagacttct gcagcagcgg ctacatcttc aaaagcatct gacgatacat
     2881 ctaaggcagc agaaataggt gcggctgttg gcggagcact tgcagttatc gctcttgcag
     2941 gcacagcagt gatcgttgca aagagaagaa agtaatattc ggaggtatgg ctatgaataa
     3001 tgcaaaaaga gttttgacat ttgcccttgc agtttgctca ctttcagcat ttgcatcttg
     3061 cggaagttca agtgattcat caagcaaggt agttgacaaa aagcttaagg aagatcagca
     3121 gcagatagtt caaagacttg ctgatgatat gaaagatgtg agagatctca aaagcaacga
     3181 aataaagtgg ttttcacatt gggatatcaa ccctactgag gcagaggaca aggagatagg
     3241 cgctgacctt gctttgttca agacaaagta taatggtaag ataaactggg tgcagaccac
     3301 ttgggaaact aagtttgacg atcttgcggc acttgtaatg tcaaatgatt cacctgattt
     3361 catcggtgct gatgatatgg acgttttccc taagggtgct ataaagtcaa tgatagagcc
     3421 tattgacgat tatatcgatc tttcaaagcc tctttggaca gacataaagg gtgcgacaga
     3481 tcagtttatg cttggcgata agcattatgt gagcgttatc agaacagacc ctgcatatgt
     3541 ttttgtatac aacaagcaga caatggaaga taacggctat gacgatcctg ctgagctttt
     3601 caaagaaggc aagtggaact gggatacatt cacagaaatg tgtcttgatt ttactgacgc
     3661 tgaaagcgat aaatacgctc tcgacggctg gtattatgaa aaggctttgc agcagtcttc
     3721 aggtgtgccg ctcatcggta tcacagacgg caagcttgta aataatattt ctgaccctat
     3781 gatagcaaag gctcagaata tgatgtatga gctgcagaag aacaatgttg tttatccaaa
     3841 gcacgagaat aactggaaac tcagaggcga cgcagagggc agcggaatgg caacaggtct
     3901 gacgcttttc tatcctatcg gactttgggc gcttgagaat gctccgtcaa caactgtaaa
     3961 ctatggcgat gtatcaaagg gagaggtaat gttcgttcct gttccttgct cagctgatag
     4021 tgataaacag tatatccctt caagagtaca cggctttagc attgtaaaga acgctcaaaa
     4081 tcctgagggc gtggcagctt tccttgaatg ttgccgttat gcagagcttg acgaggctgc
     4141 acatcagata accctcgatc agtacaagga cgattacggc tggacagatg agatgcttga
     4201 aatgagagaa actatctatg atctgtctgc acagaatcct gtatttgact ttgaacaggg
     4261 cgtatctgcc gaccttaact ctatctgcga tactgccata agaggaacaa tgaatccgca
     4321 ggagtcaaag agctggtcac aggtcgttca ggaaaatgaa aaggctatag attatcttat
     4381 cgacgaggct atgacttcaa tgaaagaggc taaataatat ctaattatca gaaaaaaggg
     4441 cggcagggtg atttgccgtc ctttttctat aaatatgagg ctttacttgt gtctgtaaat
     4501 atgatataat aagctaaaaa gcagggggtg aaagctatgg aaataaacag agaattgtct
     4561 aagcggcagt ttatgcaaaa ggaaaatgac tatcatcatt caccatatga aacggaactg
     4621 gaattttatt ctgcggtgcg agcaggcgac cttgaaacag tcaaacgttt gtatacgccg
     4681 ctggatacaa acggaaaggg cagactcagc aaagacgacc ttagaaacgt caagtatcat
     4741 cttgtcataa ccatagctat gctgtgcagg ttttgcatcg agggtggact tgacccggaa
     4801 actgcatata ccataagcga tatatacatt aataaatgtg atgagtgccg caatgaagaa
     4861 agcgttatgg ctgtacacaa agaggtcatc gtttattata caaagcaaat gaaaaaggcg
     4921 gcgtcacaga acgtttactc gaagcatatc cttatgtgct ttgactgcat tttcgataat
     4981 atttacaagg gcgtgaccgt caatcagata gctgatgaac tgaacataac tcctcagtat
     5041 ctttcaaagc ttttcagaga ggaagttggc gtaaagatct ctgatttcat tatgtcaaaa
     5101 aggatacaag cggctgaaaa tatgctgaga tattctgaat atagtccgct tgatatagcc
     5161 aattatcttg ctttcagctc gcacagccat tttattgcct gctttaagaa acagacagga
     5221 cttacgccga aacagtatag agatcagttt ttccgtgcaa actggaacaa gcatagcaca
     5281 agcagatcga aaatataagt tgatgttaat aatctgatat ttgcattact tttctgatat
     5341 aaatatcaca tttgttgatt tataatactt atagaagata aaattctgat atttattcgg
     5401 aggtaatgaa aaatgaaaaa agttttggct atgtcgattc tcacaatgat agttatgtct
     5461 atgggtggct gcggtgcttc atctgacagc agttcttcgc aagatgtcag cagcaaggcg
     5521 gaaacggaaa gcaattctcc atacacggtt gaggcagatt tcagcaaggg tgcgtcaaat
     5581 gatttttcca tttcagcagg ctggtcaaat ggtgatccgt tcaactgctc gtggagcgag
     5641 gataacgtga ctttcaatga cggaaaaatg cagcttacca ttgacagtat gggcgattcc
     5701 gaagcgtata gaggcggcga gtatcgttca aaggaaaact atggctatgg actttatgag
     5761 gtatcgatga aagccataaa gaatgacggc gttgtatcat cgttctttac atacacaggt
     5821 ccatcggagg ataatccttg ggacgaaata gatgttgaag tccttggcaa ggatacaaca
     5881 aaggtgcagt tcaattacta caccaatggt gttggcaagc acgaatatat gtatgatctt
     5941 ggatttgacg cttctgagga ttttcacact tacgcatttg agtggaaaga ggacagcata
     6001 acttggtatg ttgacggaaa ggaagcatac aaggctactg aaaatctgcc tgttacgcct
     6061 ggtaaaataa tgatgaatgc gtggaacggc attggcgttg acagctggct gaaagccttt
     6121 gacggcacag ttccgcttac agcggaatat gagtgggcaa ggtttacggc agaaaagtaa
     6181 agtgatccgc atattatgct tgcgtttatg tcttgatttt ttttagttaa tgatattgat
     6241 atttgaaccg accccattcg ctaggtcaaa aagtctagcg aatggggtcg gtttatttta
     6301 caaagataaa ctccatatat taacttttct catgagcctg actatttatg atatcatgag
     6361 tatatttctg tataggggcg ttttttgcta taattttcat ttatattctc acatctcaac
     6421 ttctcggtta tacgcctcag ccccctcgac atacagtgtt ctcatttctt caaacgagtc
     6481 gcttggggat aggataacg
//
LOCUS       DAWEMR010000145         6476 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4784_length_6476_cov_9.788506, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000145 DAWEMR010000000
VERSION     DAWEMR010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4784_length_6476_cov_9.788506"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2112..2121
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2051 a         1280 c         1479 g         1656 t
ORIGIN      
        1 tatcggcttt acgggtacgc ctatttccag agaggacaga aatacccgtg aagtattcgg
       61 agattatatt gacatctatg acatgacaca ggctgtcgag gacggtgcga cacgtcctgt
      121 ctactatgaa agccgtgtca tgaagctgaa actcgatgaa aacactctga aactgatcga
      181 caccgaatat gagatcatgg cgcagaatgc cgatgctgac gtaatagaga aaagcaagcg
      241 tgagcttggg caaatggaag ctatcctcgg tcacgatgat acgataaagt cccttgttga
      301 cgatattctt gaccattatg aaagctatcg tgagaacctg ctgacaggta aagcgatgat
      361 cgttgcatac tcccgtgcta tcgctatgaa gatatataag cgtatccttg aaattcgtcc
      421 gggctgggag gaaaaggtcg ctgtggtcat gacggcttct aacaaagatc ccgaggaatg
      481 gcacagtatc atcggcaaca agcgccacaa ggaggagctt gcaacaaaat tcaaggacaa
      541 tgacagtcct ctgaaaattg ctatcgtggt cgatatgtgg ctgacaggct ttgatgtgcc
      601 gtcccttgcg acgatgtatg tctataaacc gatgatgggc tataatctga tgcaggctat
      661 cgcccgtgtc aacagagtgt tccgtgacaa ggagggcggt cttgtcgttg attatgtcgg
      721 tatcgcaggt gcattgaagc aggctatgaa cgattatacc gttcgtgaca agaagaatta
      781 cggcaacacg gatattgcaa aggtagcata tccgaagttt caggagaaac tgcaagtctg
      841 ccgtgatctg ctccatgaat atgattattc tgcatttttc ggcggtaatg agcttacggc
      901 aggacgcacg atcactggtg cggtcaattt tctcatggac agaaagaggg agaaagacag
      961 agaaagctac acaaaagaag ctctgctgct gaaacaggca ctttcgctgt gttcctctat
     1021 cgctgaggaa gatgagcgtg aagaagccgc cttttttgaa gctgtccgtg tcctgttgat
     1081 gcgtattctc aatcagggcg gaggtaaaag gctctcactc cctgagatca atgccaaaat
     1141 caatgagctg ttgaagcaga gtatcaagag tgatggtgtt atcaaccttt tctccgacaa
     1201 ggaacaggac ttttcgctgt ttgatccaaa attcttggag gagatctcca aaatgaaaga
     1261 aaagaacctt gctgttgagc tgctgaaacg cctgattgct gaacaggtac atatctacaa
     1321 gcgaacaaac cttgtaaaat cggagaaatt cagcgagatc atgcaaagtg ctatgaaccg
     1381 ctacctcaac ggtatgctta ccaatgagga agtcattcag gaaatgctca accttgcaaa
     1441 gcagatcaga gaagcacaaa aagcaggcga agatctcggt ttgactgctg atgagcttgc
     1501 tttctacgat gcactcacca aaccgcaggc gataaaagac ttctacgaga acgaggagct
     1561 tatcgctatc acaaaggagc ttgccgatac gctccgccgt aatcgcacga tagactggca
     1621 gaagcgtgag agtgcaagag ccagaatgcg aatgatgata aagaaacttc tgaaaagtca
     1681 tcgctatccg ccggagggta tggacgatgc ggtggctact gttatgcagc agtgtgagct
     1741 gtgggttgac aacgcaagta tgaattgata taggagatga aattatgctg aaaaaagcaa
     1801 catggccgct atttacactt tcaaaaaaac gtaaaagaat agatactaat cctgattatc
     1861 agcgaccagc agtatggaca aaagcacaaa agcaactatt gattgattca attttacgag
     1921 acttcgatgt acctaaaatt tatttgcacg aaaaaaacaa tgacacatat gatgttatcg
     1981 acggtcagca acgtattcgt gcaatatggt cgttctatga tgatgagttc gctttgccta
     2041 aagatgctga acctgttaat ggttatgatg ttgcaaacaa aaagtatagc gaacttgata
     2101 tggatattgc annnnnnnnn natagatagc tataatttgg atttcgttat acttgataca
     2161 cagaacgaag atgaaatacg agaaatgttc ctgaggcttc aaaatggtac gactttaaaa
     2221 gctcaggaaa aaaggaacgc aatgcccgga cgaatgaggg ttttataaaa gagcttacta
     2281 atcatgaatt cttctcgaaa gtcaattttt ccaatactcg ttttacttat gatctcatag
     2341 ctgctcaaat gacacttctt gaattgaaca agggtatttg caatataaaa gatagagatt
     2401 taaatgcaat gtatgaaaaa tcaaagaatt ttaatacttc atccactgat gccaaaacaa
     2461 tatatcgcat tttggattat cttaataaaa tgtttcctgt caaaacaccg gagttgaaaa
     2521 gatacagcac catttcacta tttatactaa ttatggattt aatgccgaac tatgatattc
     2581 gtgagcgaga agctgatatt gcaaagtggt ttattgattt tgaagctgag agattattag
     2641 acgaacataa agaaccagaa aagcaagatc cacgtcttgt agtctaccat gaaagagtat
     2701 ctcattcatc tgactcggaa gattcattac tttacagaca taatttcctt aaagaaagcc
     2761 ttttaaccca tgttcctaat cttccacaaa aagatcctaa aagaagtttt gacgaggcgc
     2821 agcgtcaagt catttatcga cgtggaaacg gtatttgcaa aatatgtggg gtaaaatgta
     2881 attggaataa ctgggaagca gatcatatta ctccatggag taagggtgga aaaaccgaaa
     2941 tagagaatgg acaggtactc tgtccttcat gtaattcaaa gaaaagcgat tcgtaagaaa
     3001 taatatccaa caaaacttag tctattggtg cacacaaaac ctatcccaca caactcacat
     3061 tcagcattgt ggtatatctc ataatcagtg gatactaaaa tgatacatag caacattaaa
     3121 gcaccctctc ggagcgttcc tgagagggtg ctgttttcat cggtttgttt tccgctcggc
     3181 aggctcggac ggcgttgagg ggcattttcg ttcgtcacag tccgcaccac attgctgata
     3241 agctcagcga ggacggtgta ccaattccct cagattacta ctacgcaaag cttggcaaac
     3301 ctaatcctcg ccgtacaagg catatgtgga gccgtgaaac agtcaagcag atacttcaca
     3361 attacaccta cctcggacat cttgttcagc tccgcacaag aactgtcagc tacaaaaatc
     3421 acaaggtcat caaaaatgac gatgaagaca tgatcgttgt gaagaatacg cacgaagcta
     3481 ttgtttctca ggagctttgg gacagggtgc gtgagatgga gcaatcggtg tcgcagggca
     3541 agcgtaacag tagtggctca gcggtttgat ctactgtcag gactgcggaa acaaagtccg
     3601 ccttgcgtgg aacaatacca caagcggcag taagaagaag ccgagaaagt atatccacca
     3661 caattacaac tacacttctt acatgaaatt cggaaagcga tactgtccca gccattacat
     3721 taaaatgcag gatatggacg ctatcgtgct taaagatatt cgcagtctcg cactagagga
     3781 cgaggaaaag gcaaaagcgg aatctcttgc acgcaaggcg aagcatcatg aagagcagta
     3841 ttccgtggac accaagaagt tcacagaagg cggataccgc ttacaggagc ttacccgtga
     3901 gatgtgtctg gtatcacgct tgatgcctat gtcgaggtgc agccccgtga gatctacatc
     3961 tactacgagc tactcgatga acccctcaag gacaagagaa gcctgtttta aggcgtattt
     4021 acgttattta tcaaccatac gaaagtgtgt taccatttgg caaggggact accgctgatt
     4081 tttacagtca cttggatttc aactctaaga ttgactcagc taatacgatc gccaatatcc
     4141 tcggtggcgg agctgtcgaa aggccgcccg ctacggaaag caagagtgta ggaagcccta
     4201 atacggctga tgacatgaag aaataaaata aatgtgactg attggtagat aaacggacaa
     4261 aaaaataaaa tcggacttca aaaaagcccg attttacgat gatttatggt gccgctaacc
     4321 ggacttgaac cggtacgggt tttaccccga gggattttaa gtcccttgtg tctgcctatt
     4381 ccaccacagc ggcatgcaag atatattata acactttttt tgcaaaatgt caacgctttt
     4441 ttgcaaatta caagttgtta aacggaaata agtggtaatg acagaatagt ggcagtgttt
     4501 attttaaatc tttttgcctt gaaactgaaa atctgtagat aaatacagat atcaaagcgg
     4561 tgacgacttc tgttatccag aatgcgttcc atacgcctgt tggtcccgtg agcttgcaga
     4621 ggaagaacgc aagtggcagt attatcgcaa taaatctgca cagagatatc ataagcgacg
     4681 gaatgcccat gccaagcccc tccagcgcac ctgatgaggt gacggatact gcggagataa
     4741 caaagccgat acaaattagg cgtagtgctg ttttgcctat ggcgatagtg tcagcgtttt
     4801 ccgtgaaaag ccctatgagc ttggctggga aaagtgcgca tatgatagtt cctgccgcca
     4861 atatcacacc tgtcatgtaa agaacaatgc ggtatatttt cttcacacgc tccttctcgc
     4921 ctgctccgta gttaaaactg ataactggtc gcataccctg aataacacca tttgcgggta
     4981 gataaagaaa ggtctggagc ttgtagtaaa ttccaagaac aagcacatag ctttgtgaat
     5041 atgccgcaag tatgccgttg agggcggata tgagaagtga cggcagtgca agattaagtg
     5101 ttgcaggaat gcctatggag tatatctttg cgattattat gccgtcagga cgcttgtgtt
     5161 ttgcggaaag cttaacagga agctcacagc gaagatatgc tattatgtat atgacaaggg
     5221 tcagcacctg accgataccg gtggcgagtg cagcaccctt tatacccatc tcaggaaaaa
     5281 atccaacgcc aaaaataagc agagggtcaa ggattatgtt tgcaatacag ccgcagagca
     5341 tactgaacat tgatatcttc attctgccga cagactggaa tatcttctca aatgcaatgg
     5401 caagcattaa tgcaggagag aatgtaaata caatgttgca atactgcacg ccaagatcta
     5461 taacgttttt attacttgtg aacatagata gaaatgcagg cattataagt atacatatta
     5521 tcattagcac aataccatgc aaagccgaaa gggcaagccc ctgagttgcg gcaatgtcag
     5581 ccttttcctt tctgcctgca ccaagatata tggcaatgac cgcattgatg ccgataccaa
     5641 agccgatagc agtggcactt ataaaattct gcacagggaa caccagtgaa agcgatgtca
     5701 ttgcgtcctc gcttatcttt gccacgaaaa agctgtcaat gatgttgtaa agagagttta
     5761 caagcattga tatcaccatt ggcagggaca ttgaaagcag aagagggaag atcggtcttg
     5821 ttttcatgaa atcactgttc atttttttta cactcctttg tctgtgggca gaaaaaaagc
     5881 cgcacagcct gtttgctgtg tggcgaaaag tgacatactg tcaggaaaaa tccacgcaag
     5941 ttttacaaag tatattttaa catagcggta tgattttgtc aataaatttt ctttaatatt
     6001 atgtttcaaa aaagtataca aacacgctaa ttgtagattg acaagatttt tctgctatgg
     6061 taaaatgtta ttatgataat ttatgttagg ggagaaagcg taatgacttt tatagagtgg
     6121 ctcaaagact gtaatgaagc agaccctttg actaaaaagg tttacatatg taatgattat
     6181 ctcaacgccg agagaatgtt ggagaacgca agcggtgaaa atattgttat aaggcttgaa
     6241 agatacacag tcgcagatca tgcaaagcat attatcgaaa ccagtgccga gtatatggac
     6301 agcaggataa taacgctgtc aaatgctgag ggctgtgaga tagtaagacg tattattgat
     6361 gaaagaggta taagctattt caaaagcgac gaccataatg cctgcatgga gatattcaag
     6421 acgatatcag agcttagaat gaactgtata ggccctgatt cattgtcctt ggacag
//
LOCUS       DAWEMR010000146         6377 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4847_length_6377_cov_11.752610, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000146 DAWEMR010000000
VERSION     DAWEMR010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4847_length_6377_cov_11.752610"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1894 a         1361 c         1457 g         1665 t
ORIGIN      
        1 atcttcatgt catttgcagc gctttccacc gttatcgcag tgtttgagaa cattatcaca
       61 atgtcggtgg aactgtggga ttggagcaga aaaaaatccc ttattgtaaa ccttgtgatg
      121 atacttgtgc ttacaatgcc tgctgtgttt ggatttaacc tgctgaaaaa tatccagcca
      181 atgggcgcag gttcgactat catggacctt gaagacttct tggtatcatc gaatcttctg
      241 ccacttggct ctcttgtggg agtgcttttc tgcgttcgca aaaatggctg gggctgggag
      301 aacttcatca aggaagccaa cactggcaag ggcaagcata tccctaactg gcttcgcagc
      361 tacatggcct acgttgtgcc tgcacttata tgcttcatct atcttaaagg atactatgac
      421 acattctcaa acagaagtct gccggtcctt ataggctgga tgtgctttgc ggtgatactt
      481 cttgtgctga tattctgggc ggctttctca aaaagcaaaa aaaatttgga aaatcctatt
      541 gacaaataga aagtttggtt atataataaa ccttgcgtga tgtttcacaa aaaatcgtat
      601 aatgataaac gctataaaaa taaactaaaa ggtggtgggt gaaatgagaa atattctttt
      661 tgatcttcta aagactaatg agaatacaag ccttacaaac aatgtaagtt caatatgcct
      721 ttcagatgtc attttgcctg cccttattgc caaaagctaa tgagattttt atcagctttc
      781 tggatattat actgcagaac tattgcgacc tctgatatca ggtatcagag gtcttttttt
      841 gacctgttat aggcagttac attatgcgaa tttacagaat tatatcggag aaatatgaaa
      901 tttatcccga aaaggaggga atacatgaaa ttttttgacg ttttgccaca gcttgaagaa
      961 aacgaactgc taaagcgtgg agttatgact gacgagctgc tttactgcgt aaaagctgac
     1021 cttgacggtg agggcggata tgtggacgta tacataactt ttacaaaaga caccctttgc
     1081 atactccgtg gctatgaaga atacggaaaa atgaagcgtg ggcagaagag aaaaaatctc
     1141 gtaagcttca cagtatcagg ctatgatgaa taccccatag acagcattga agaaatgttt
     1201 gttgaccgct atgcaaattc cgcaagactt atgataaagg acaagtcagg caaggaattc
     1261 cctatagcga ggttctcact tggctttgct gacaaatttg agaaattcag ccagcgtgtg
     1321 aactgcacag ccaagaacga gcctattgac gaccgcctgc tggaaggagt aaagacccac
     1381 tgtcctacct gcggtgagcc ttatcctgac cctaaccgcc ctgtctgtcc gagatgtgta
     1441 gacagacgct cggtgttcaa acgcctgctt tctatgttcg gtgaatttaa gggtgcggta
     1501 tttatcatac tgctcaccat agttttaagc aacgttttcg cagtgctttc gccgatattc
     1561 ggctctcaga tgctctatga tgatgtgctt gcggaaaacg gaaaatacta tggcaagata
     1621 ctcatgatag taatgctcat cgtgggcata aacattgcag gaatgataat gcgtatcatt
     1681 tcaggcatta taacctcaaa ggtcgtgcct gttgttacgc accggcttag ggtgaagata
     1741 ttctcctcaa tgcagcggct ttcccttgac tttttcacaa gcaaacagac aggctcgctt
     1801 atgtcaagag ttgaccgtga tagtcagaac atctactggt ttttcacgga tatagtgccg
     1861 tatggtctta caaacgtggt aaagatagtg ggcattgcgg tgataatgct cacaatttcg
     1921 ccacttctgt cacttggcat aatcctcact atctcggtga tagagatagt tcagcaccaa
     1981 ttttacaaaa gccagcgcaa gctttaccgc aaatatgacg ttgcaatgcg ttctgcaaac
     2041 tcggtgctgt ctgacgttat gaacggtcag cgtgtggtaa aggctttcgc ccgtgaggac
     2101 agagagatag aacgctatag ggtaaaaaac actgacgcat ttttgataaa ctcaaggata
     2161 aactcacgaa tagcaaacac tatccctgtt atatggacgt tctataggat agtgaacaag
     2221 cttgttttct gccttggcgc agtgcttgtc ataaaaggtc acatactatt cggcgcactt
     2281 tctgcactta tctcatatac cgaaatggcg atggatccta tcaacttttt cacatggata
     2341 ggcgaccgct gggcaaaatg tatggacgcc gcatcaagaa tgtttgagat aatggacgct
     2401 ctgcctactg taaaagagga cgaacaccct gttcatatgg aaaacatgaa gggggatatc
     2461 gagcttaaaa atgtatcctt tgaatatgaa gcaggacacc ctatcataaa aaacgtttct
     2521 ttcaaggtgc aggctgggca tatgtttgga atcgttggta aaacaggcgc aggaaaatcc
     2581 actatcataa accttatggc aaggctttat gacgtaacag gtggtgagat aaccattgac
     2641 ggtgtgccta tcaggaagat agcttttgac gatctgcgca gaaatatcgg tatagtttct
     2701 caggaaactt tccttttcat agggtcgata gctgacaata tccgctatgc aaaccctgac
     2761 gcaaccatgg aagaagttat cgaagcggca aagtccgcaa acgcacatga gtttataaca
     2821 aagctccctg agggctataa cactaaggtc ggcgagggcg gaatatcctt gtcaggcggt
     2881 gaaaagcaga gaatatctat cgcaagggct atcattcaga agcctaacat acttatcctt
     2941 gacgaggcga ccgcttccat ggacacaagg acagagcgaa agatacaagc cgctatcgac
     3001 aatctgaaac agggcaggac gattatttct atcgcacacc gcctttcaac tctccgtgac
     3061 gctgatatgc tctgcgttat cgaaaatgga gagcttaaag aaatgggaac acatgacaag
     3121 ctcataaaag aaaaaggcaa atatttcgag ctttacaagc tgcaatctga agcattgcag
     3181 ttcataaacg aatagtaagg agatgaacac aatggctgaa aatattaccg ctgagcagga
     3241 ggtttatgag gtcgataagc tgaccttgct tgactgcaaa aagatacgcc ttgcaaagga
     3301 agaaagcggt tttcttaccc tcgattatga gggcaggact tatcacaagg taaaccccac
     3361 aaggctcata ccattttact caaagaccac ctacataagc ctttcatacg aaaattccga
     3421 aaaagagttc cgtgagatag gcgtgataaa agatatggcg gagcttgacg atgagcaata
     3481 caagcttctt gacagttatc ttgaatacaa atactatatg cctgagataa caaaggtcta
     3541 cagcataaag gacaatatgc gtggtgcgat ctttgtaaag gctgacacaa cttcagggca
     3601 gaaaactatc tgtatccgtg actggtatca gaatttcaga atgataggct atgattatct
     3661 ctacgtcaac gacgcagacg gcaacaaata tttttgccct gacattcata agcttgacag
     3721 aaagagccgt caggtgcttg aaatgtacac ctaaaaggtg ttagaccaca ctgaaaggag
     3781 tgtgaaaata tggatctaaa aacctctctt cctgaaaacg ccaaggcaga tatcatatat
     3841 tccctgcctg caaacttcac ctgtaagggc gacaaaatgg acggacattt ctgcgtgaca
     3901 aaagaaaaga tatacattta caacggcagt gagataacac ttgaatacag catagacgat
     3961 ttctccgagt ttgagtgcaa acagcagata ggcacttcaa tggctcaggg aacgctgaaa
     4021 agcggtgaaa ctatctgctt ctgcggtttt tcgcaggatc agtttctcag atacgccgag
     4081 cttatgaagc ttcttgacca ctgtttgcgt acaggcgagc ttatggagat aaccgacacg
     4141 gaagagcctg tctgccccaa atgcggtctg ccgctggagg gcgcaaagga atgtatctac
     4201 tgcactggca agggcaagac aatggtcaag ctcataaaac gcattgcgcc atacaagaaa
     4261 tattttgcga tcgcagttat atgcacgata ctttccgagc ttatttgggt gcttgcgcca
     4321 tatcttgaca gagttataat cgacaagtat gttaccccga aaaacaccca ttgggcaggc
     4381 cttgcaggag ttatcatcac tatcgtcacc ctgctgcttt tggcaggagt attcgagttt
     4441 ttcaacatga aaaacagctt caaggtcgca ctcaatctgg gacgtgacct taggcaggag
     4501 atatttgaaa agtcacagca gctttccatg aactccatat caaagcgaac agcaggcgag
     4561 cttatcaacc gtgtttcaag cgacgccgca aagcttgagg acttcatcac tgcaaacggc
     4621 aaggacgcca tagtaaaaat actttccctt gtgataatag gcatactgct ttttattatg
     4681 gactggcggc tcgcgcttat gacagttttg cctgtgccga tagttttcat aattgtgcaa
     4741 aagctttttg atgccatggc gatacgctac acaaaggtat ggaaaaacgg cgtttctcat
     4801 ggagaaactt tgcatgatat cttaaacggc ataagagttg taaagtccta cggcaacgag
     4861 gtaagggaga tacagcgcta cacagaatgt tccgaacgct gggcgaaaag ctgtgttcgt
     4921 gcggaaatga tgtggtatct caccattcct gcctcagaat ttatcctcac cattggtaac
     4981 ttcttcgtac tgtatttcgg tggaaacatg attctcgacc acaccatgac attaggtcag
     5041 cttatacagt tcaccaccta cgttgcaatg ctttacgaac ctatacgctg gctcatacag
     5101 ataccgagaa atcttgcgga cacctcagtt tccgcaggca aggtctttga gatacttgac
     5161 gaggacactg acgttcgtga ttgtgatgac cctattgacc ttgacattca aggcgatatc
     5221 gaatttgacg gcgtttattt tggatacaag gtatacaatc ctgtgctgaa agacataacc
     5281 tgcaagataa acaagggcga aatgataggc attgtgggac actcaggtgt gggaaaatcc
     5341 acaatgataa atcttattct cagactttat gactgcacgc agggagagat acgcattgat
     5401 ggtgtgaaca taaaggatat tgcacagcac agcctgcgtt cacaggtggg cgtggtgttg
     5461 caggaaactt tcctttttga cggaaacgta cttgaaaata tagcatatgc aaagcctgac
     5521 gctacctttg aggaggtaat aagggcagcg aaaattgcga atgcacatga ctttatcaca
     5581 aagctccctg acggctacaa cacccgagtg ggcaacaagg gctatcagct ttcgggcggc
     5641 gagcgtcaga gaattgccat tgcaagagcg atactccatg accctaagat aataatactt
     5701 gacgaggcga cggcttctct tgacactcag acggagaagc agatacaaga cgctttgggc
     5761 aagctcacaa agggcaggac gacaattgcc attgctcaca gactttccac cctaagcaac
     5821 gccgacaggc ttatcgtcct tgacaagggc agacttgcag agttcggcac acacgctgaa
     5881 cttatgcaga aaaaaggcgt atactacaag cttgttatgg ctcagcgcca gaccacaaaa
     5941 atgaaaaaag cccaaccgca gacctgctaa gcacttgaca ttatcacggc aatgtggtaa
     6001 aatacaatag taaaatctca tatcggggaa tgatgttctc ccctgggaaa cctaaaccgc
     6061 ttattaagct gatgacttct gtttttacgc aggagttgtc agcttttttg atttttggga
     6121 ggtataatta tgttattttc actggctgtg atctttcttg tggggctgtc agccgccgcc
     6181 atttttcagg ctatcggtct gccgaggata atcggtatgc ttgcaacggg aattctcaca
     6241 ggcacttacg ttctcgatct gctggatcct aagatacttg gcatatcctc agagcttcgg
     6301 cagatagccc ttatcattat ccttatcaaa gcagggctgt cactcaattt gtccgacctg
     6361 aaaaaggtgg gcagacc
//
LOCUS       DAWEMR010000147         6340 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4871_length_6340_cov_12.588385, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000147 DAWEMR010000000
VERSION     DAWEMR010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4871_length_6340_cov_12.588385"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1949 a         1304 c         1579 g         1508 t
ORIGIN      
        1 tacatatgcc ctttcgcagg caaagcaatg tctgaatatt tcatgtatta gggcaaggat
       61 gtacttatcg tatatgatga cctttcaaag cacgcagttg catacagagc aatgagcctg
      121 cttctgaaac gttctccggg acgtgaggct taccctggag atgtattcta tcttcattca
      181 agactgctgg agcgttcatg cagacttgac gacgctcacg gcggcggttc tatcactgcc
      241 ctgcctataa tcgaaacaca ggcgggcgac gtttccgcat atatccctac aaacgttatt
      301 tctatcacag acggacagat cttccttgaa acagagctgt tcttctcagg cgtaagacct
      361 gctgtaaatg ttggtctttc agtatcccgt gtaggcggtg cggcacagac caagatagta
      421 aagaaagtat caggcaatct gcgtatcgac cttgcgcagt tcagagaaat ggaagtgttc
      481 acacagttca gctctgagct tgataagaca acccaggagc ttctcgacca tggtcacagg
      541 ctcgttgaga tacttaaaca gccgctctat cacccttatt caatgcacga ggaggtaatt
      601 atcctctgct gcgcacagca caagcttttg cttgacgtgc ctgttaagga gataagaaaa
      661 ttcactctcg atatgctttc ctactttgag gcaaatcaca gcgatatcat tgccgagata
      721 aacgacaaaa aggtcatgac agacgagctt gacgcaaaga tagttgctgc cgcaaaggaa
      781 tttaagcagg gcagataaag gctctgcacc gaaaggaggc ataaggcatg gcaggaatga
      841 gagagataag agaaaggatc gcaagcgtaa gcgagatcat gaagatcaca aacgctatgt
      901 accttatctc ttcctcaaat ctgaaaaaag ccaagaaaag gcttgaaaac accgagcctt
      961 actttgaaaa gctccaatct accattcacc acatacttct tcacacacct gagttcgagc
     1021 acaagtattt cgaccagcgt gaggagaaaa agcctgagga caaaatgata ggctacatag
     1081 tggtaacagc cgacaagggt ctggcaggag cttataacca caacgtttta aagcttgcag
     1141 agcaggaaat ggctaagcac aagaacacct gccttttcgt tgtgggacag atcggaagac
     1201 attatttttc aagaaagaac gtgactatcg acgccgagtt cctttacacg gctcaaaatc
     1261 ctacccttta cagggcgagg gcgatcgccg aaactgtcac agatcttttt gagagaaaat
     1321 atcttgacga ggtttatgtt atttacacca agatgataaa ttccatggat atgaagcctg
     1381 agatgctcaa gctttttccg ctggagcgtg aggactttga acaccctctg caaaagcagg
     1441 atcacgatat cgtgacgttc agaccaagtc ctgaaaaggt aatggatcat cttgtgccga
     1501 actacatgaa aggtcttata ttcggcgttt tggtagaggc gttctccagt gagcagaatg
     1561 ccagaatgac cgccatgagc ggtgctacca caagtgcttc ggagatgata aaggagctgt
     1621 cattgcagta taaccgagca agacaggcgg ctatcacaca ggagataacc gagatcgtca
     1681 gcggagcaaa tgctcaaaag gacgagagtt aaggctttcg ggaaaggtgt tgagattaaa
     1741 atggaaaaag gaaagatcgt tgaaataatg ggacctgtcg ttgacgttga atttgaaaac
     1801 ggcaagctac ctatgataaa ggacgctctt actgtacagc ttgacggcaa gacaaaggtc
     1861 atggaagtgg ctcagcagct tggcaacaac actgtcagag ctatcatgct tgcttcaagc
     1921 gacggacttg aaaaggatat ggaggtcact gccacaggcg cttgcataaa agttcctgta
     1981 ggcgacgcta cccttggaag aatgttcaat gtccttggtg agagcattga cggcggcgag
     2041 cctgtcaagg ctgatgatta ttgggagata cacagagagc cgccaaaatt tgaagatcag
     2101 aagcctgcaa cagagatcct tgaaacaggc ataaaggtaa tagaccttct tgcgccatat
     2161 gcaaagggcg gtaaaatagg tctgttcggc ggtgcaggcg ttggtaaaac tgtgcttatt
     2221 caggagctta tcacaaacgt tgccacagag catggcggat attccatatt cacaggcgtt
     2281 ggagagcgtt caagagaggg caatgacctg tggcacgaaa tgacagactc aggcgttatc
     2341 aaaaagacag ccctagtgtt cggtcagatg aacgagccac caggatcaag aatgagagtt
     2401 gctcagacag gacttacaat ggctgagtat ttcagagata aggaacatca ggacgtactg
     2461 cttttcatag acaacatatt cagattcgtt caggcaggct ctgaggtatc tgcacttctt
     2521 ggcagaatgc cgtcagccgt tggttatcag cctacccttg cgaacgaaat gggcgctttg
     2581 caggagcgta tcgcttccac aaagcagggc tctatcacat ctgttcaggc tgtatacgtc
     2641 cctgcggacg acctgacaga ccctgcacct gctatcacgt tctcacatct tgacgcaaca
     2701 acaatacttt caagaaagat agttgaacag ggtatctatc ctgccgttga cccactggag
     2761 tcaacttcaa gaatacttga gcctgacgta gtaggtcagg agcattacga agttgcaaga
     2821 aagactcagg agcttttgca gaagtacaag gaacttcagg atatcatttc tatcctgggc
     2881 atggaggagc ttgacgacgc cgacaagacg gctgtttaca gagcaagaaa gatacagaaa
     2941 ttcctttcac agcctttcca tgttgcagag aacttcacag gcatagaggg caaatatgtt
     3001 cctctgaaag aaacgatcag aggcttcaag aagatcatcg acggcgaaat ggacgaatat
     3061 ccagaggctg cattccttaa cgcaggcact atcgacgatg ttatcgaaaa ggctaaacag
     3121 cttgaaaacg agtgatgacc ctcaccgaaa ggagaagctg aaatggtaaa gacattcaag
     3181 cttgaaatac ttgccagcga ccacattttc tattcgggag aatgtgacga gcttatattc
     3241 ccgggtcagg acggctcttt cggaatactg cctaatcacc agcccatgat aacctgtctt
     3301 aacgcaggta agctgcgtta cagaacgggc gaccagtggc actatgcggt ggtaagcgat
     3361 ggttttgtgg agataatgcc gtcatatgtg acgcttcttg cggacactgt ggaacgtcct
     3421 gaggagatag acgaaaagcg tgcggaagcc gccaaggaga gagctgagga gaagctcagg
     3481 cagaagcaga gcatttcaga atattatcac acgcaggcag ctcttaacaa ggctgtggca
     3541 aggcttaaga tatcaagcaa gtatacaaaa cataagataa atatttgata caaaggctct
     3601 ccgctcacac ggggagcttt ttttatgttt gcgtgttacc tttttaaaaa atgcgtgtat
     3661 atattagtga aagccaaaag acaggaggtg gaaagcttga acgaaaaaca tctgataaca
     3721 aagctgaaaa agagatccca gaaagctctg gatcagctta taagctccta ttcggcttat
     3781 gtgtatacca tagcgagaaa cattctggga ggtgcgttca ctgcagagga cgcagaggag
     3841 ataacatcag atgtgttttt ccgtatctgg cagaccgccg acagccttga cgaggagcgg
     3901 agcatttcac cttatcttgc agcctgcaca cgaaactgtg ccttgaacaa gctcaggtca
     3961 aagggcaagg atatgcagtg cggcgagctt atggaggata tcttatcctg cggagacatt
     4021 gaaaatgata tggaaaatgc agaacagctt gccataatat ccgacggtct tgaacggctt
     4081 tcaaagcagg acaaagagat ctttataaga ttttactatt acggagaaag acttgccgcc
     4141 ataagcggaa aaatgggcat tacagaaaac gcctgcaaaa caagactttc acgcacgaga
     4201 aaaaagctta gagaatatct ggcagaaagg ggctataaaa atgaaaaaaa atgataactg
     4261 gtcgcagagc tttgcaccgc tcaacacggc agaatgtgac cccgtccgca ttgcaggctc
     4321 tgtcagagaa aagataaacg cagagaaagg caggaacata acgatgaaaa caaacaaaag
     4381 aagaataaaa ccgcttgtta tcgcagcagc agttgcagcc accgccgcag tttctatggt
     4441 atcagtaaac gccgccacag acggacagct tgttgagaag ataagtctat tcataaatgg
     4501 caaagagcag gatatcgaca actcaaatat caagaaaggc gttgacgaga acggcgatca
     4561 gtattatgaa tttcacgtca atgaggacac ggacagcgag gtatctgtgg agcttagggg
     4621 agatgtttcc gatgctgatg ataacggcgt tcagtacgat atgaatgttg acaaggctga
     4681 ataacgccag ttcacaaaaa gtttacatta aaagcatagc aggctcttga caaaaggtca
     4741 agggtctgtt ataatattag agcattcgta tctaaagaca gttggcagca ggaaactgcg
     4801 gaagtccagc cgaggaaagg aatagcaaag taattatgac tttgtatctc ttttgtcttg
     4861 cgacgaaagg gatttttctt ttgttagttt acggtttgcg taaattagtt caaagaggtg
     4921 aaattgaaat gccaagcgaa aagacattgt ccgctaagaa ggaaagagtt gcacagctcg
     4981 ttgaaatgct caagaactct gctgcaggcg tactcgttga ttacaagggt atcactgttg
     5041 aagaggatac aaagctcaga aaagagctga gagaggctgg cgtaagttat ttcgttgaga
     5101 agaacactat cctgagattt gctcttaagg aagctggtct tgacggtatc acaaacgtac
     5161 tcgagggtac aacagctatc gctatctcta acgacgacca gacagctcct gcaagaatac
     5221 tcggtaagtt tgctgaggac tgcaaggacg aaaagttctt cctcaaggct ggttacattg
     5281 gtgaggacgt ttacgacgag gcaggcgtta aggctctctc gaagatccct tcaagagaaa
     5341 ctctgctcgc acagctggtt ggttcccttc agggtcctat ccagaagctt gccgcaacac
     5401 ttcaggctgt tgtggacaag gacaacgaag ctgcataatt tatatgccgc ttaacgaatg
     5461 ggctttgacc cgaaaaatta tttaataaaa aggagatttt tatcatggct tcagagaaga
     5521 ttatgaattt tgtagaagag atcaagaaac tttccgttct cgagctttca gagcttgttg
     5581 acgctatcca ggaagaattc ggtgtaacag cagctgttgc tgctgctcca gctgctggtg
     5641 ctgctggcgc tgctgctgag gagaagacag agttcgacgt agttctgact tcattcggcg
     5701 atgctaagat ggctgttatc aaggctgtta aggagatcac aggtcttggt cttaaggagt
     5761 ctaaggaact cgttgagagt gctcctaagg ctatcaagga aggtgcttca aaggctgacg
     5821 ctgaggagat caagacaaag cttgaggctg ctggcgcttc tgttgagctc aagtaattct
     5881 gagtttacac aaataattaa aggacttcgt aatgaagtcc tttttttgtt gaaaaaattc
     5941 ataatgagtg aaactgttcc accttgggca gcggagtggt ttgaattttt tacaaggttg
     6001 tgtagatata ccgtgtggta tgctttggtt tagaatatct agcccaacag aactgttata
     6061 tcgcctgact gatttgtcaa tagcgaagct cgaatatata caaacaagta tgtggtatgt
     6121 atatttataa atagtcgaat ttttttgaaa aaaagccttg acaatagaaa aaataaatgc
     6181 tataataatt aagctgaatg acacggaaca caaacaaaac gaaagagttc agcgaaaaga
     6241 atagcaaatg cggcagtgct ggaattggca gacaggcacg tttgaggggc gtgtgtcctt
     6301 gacgtacggg ttcaagtccc gtttgccgca ccatacttgt
//
LOCUS       DAWEMR010000148         6245 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4959_length_6245_cov_12.165590, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000148 DAWEMR010000000
VERSION     DAWEMR010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4959_length_6245_cov_12.165590"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1448 a         1578 c         1251 g         1968 t
ORIGIN      
        1 gatagttttc agagcttcat ctgtatatct caggctgata tgtttctcct ccagcggcga
       61 cttcatctca tccagcataa gacctgctat gcctgcgtaa ttctcctctg tgagcggatt
      121 gaacacaact atctcgtcta ttcttccaag aaactcaggc cgcaggaaat ctctcagggc
      181 tttcattgct ttatccttgg cgatatcact gtcggtctta ttgaagccca cacctgtatc
      241 tctatcagtc gaacctgcgt ttgatgtcat gacgataact gtgttctcaa atgaaacgct
      301 tctgccctgt gaatcgttga tacgtccctc gtcaagtatc tggagaagta tgttcataac
      361 gtcagggtga gccttttcta tctcgtcaaa aagtaccact gaatatggtc tgcggcgcac
      421 cttttcggta agctgacctg cttcatcaaa gcctacatat cccggaggtg agccgataag
      481 ctttgatact gagtgcttct ccatatactc ggtcatgtca actctgataa gcggctcggt
      541 ggcgtcaaaa agctcttctc caaggacttt cacaagctct gtttttccaa cgcctgttgg
      601 gccgacgaaa ataaatgaag caggtctgcg acgttttgaa agctgaaccc ttgtacgctt
      661 tattgccttt gcgaccttat caacggcttc gtcctgaccg ataacacgct ttgaaagggc
      721 ttctttgaga tgctttatct tatcatactc ggtctgagct atcttattgg cagggatacc
      781 tgtccaaagc tctattacct ttgagatatc gtcctctgtg acctgaacgt ttttaagagt
      841 ttcttccacc tctttgagtc tgttctggat cctgagtatc tcgccctttt cctttgcgat
      901 tatctcatag tcagggtctg acttctgctc gtaatcctca acgagctttt catgcttttt
      961 aagctcctcg ttaagtgcgt cgaactcctc tatttcaggt gttctgatac ttgtgcaggc
     1021 gcaggcttcg tcaagaagat ctatcgcctt gtcagggaga aatctgtcgt tgatatatct
     1081 ttcagaaagc actgcacact ctctgagcat atcgtctgat atctttactc tatggaaatt
     1141 ctcgtaatac tgctttatgc ctttgaggac tgtgattgtg tcctcaactg tcggctcgga
     1201 aactgttaca ggctggaaac gtctttcaag tgcagaatcc ttttcgatat actttctgta
     1261 ctccttgaaa gtcgttgcgc ctattacctg tatctcgcct cttgaaaggg acggtttcag
     1321 gatattagct gcgttcatcg ttccctcatt gtcacctgtg ccaacgaggg tatgcacctc
     1381 atctataaag aggataacat tgccctgctc ttttacttcc tctacaagtc ctttacaacg
     1441 gctctcgaac tgtcctctga actgagtacc tgcaacaagt gcggtaaggt cgaggaggta
     1501 aagctcctta tccttaatat ggaacggcac atctccgcca actatcctct gtgcgatacc
     1561 ctcggcaatg gctgttttac caacgccagg ttcgcctata aggcatgggt tgttcttctg
     1621 acgtcttgac aggatatgga taactcttga tatctcctta tctctgccca caacattatc
     1681 aagcttgccc tctcttgctt tctgagtaag atttgtgcaa tagttgttga gaaatttaag
     1741 ctttttcttt ttatcctttg gcttgttctc tccgccctca tcggtggctt taggcatacc
     1801 tcctgcgagg ttgctgaaaa gattggctgc gtcaccgccg aaaagattgc tcaggaagtt
     1861 cgttccgccc ggctcaaaat cgtccccgtc ggaagcctcc ataagctgat ttgacattgc
     1921 ttcaaggtca tcgtctgaga tgcccattgc cttcatataa tcgtccacct gcgaaatgcc
     1981 aagctccttg gcacaaacaa ggcaaagtcc ctcgttcttc ttgtgctgag ggtccttggc
     2041 gttcatctgt gagatgaaca ccacagcagg acgcttttta catcttgaac acattaacat
     2101 ttttatttgc tcctttcctt actgatacac cttacggtaa tcatttatac agaatatcaa
     2161 agaagtaact gaacagatag gtattttcaa aaacagcgct gttgaacttg ggaagcttgt
     2221 tctcggtggc atttatcaca gtgcagacca taaaggctat caacaccaga tacagaccgc
     2281 ctttgcaaag accaagcgcc atgcctgcga aacggtttgc cgctgtcaga cctgccattc
     2341 gggtaaaaac tcccgacaga cgcaggataa gtcttatgac tatctccata agcacataca
     2401 tcgtcatgaa cacgcaaagc cttataaaag tcagggcgat cggtcggaaa atgttctttt
     2461 ccagatactc agcaccagtt ctgccaccct cagagagagc cttgacggct tcgtctatct
     2521 gctcggcagt gaacttcacc gccttagaca gactttttcc aagcttattg ccctccagct
     2581 tttcgccgag cttttggggc ataacgctgt ggatatactc tgaaagctcc tcgccctttt
     2641 gctgagcggt atcaaagtct gttccctttg aaacaagcag cttttcggta ttctccgcaa
     2701 gagtgcctgc ccctgacaga acgtttttta tctcctcatc tgaaaggtcg atatcaacgc
     2761 cctcgttttt cagttcattg ctcacaagct ttgaaagatc cgtctgttca gcagcgtttt
     2821 caaggctcgt tataagctgt gttttgaaat atttgttata tacattttca gccgacatat
     2881 tggctataag aaaagccaca ataacggctg ccactgttcc gagcattgtg atgacatatc
     2941 tcacaaagcc cttacagtat cctgtcagag cggtcacgat aaggatcatt ataactgctc
     3001 cgtcaaaaaa atatgccata tttccctgcc tttcgtcagg tctcgaaagc tattttgttt
     3061 atctcatgca gacctaggaa ataaacgctg tcgttgaggt aaacaaagtc aagatactca
     3121 gaggacactg ccgctgttgc aagtagattt cctgcactgt cataggcttc tatattatcc
     3181 tcgccgagaa ggatcacctt cttattctct gcggtcattt tcttaggttt gccgctttga
     3241 gtataaacaa cgctgtcagg cttatctccg tcagagtccg aacggaacac cgcaagagtt
     3301 cctgttttac gctgtatgcc gtcaaatgct actgcggcag tggtatctgt cagggcatag
     3361 tctgaaagct cctcaggata ttcatactgc ataagtattt taccattgct gtcaagctta
     3421 tagaagcagg tatcgcccac tacccaataa tcgccgttat cgtttcgctg aacggcaact
     3481 gcgagagttt caagctgttc gctcttcatc ttctcctctg tgctgtcaaa ggtcacataa
     3541 tagatgaccg agcgtaacgc tccgccactg ctggaaaatg ttgagataca acagccgcta
     3601 ccgtccttat caaagcttat atcaagtatt cttcgtgagc ttgaccactg atatatctct
     3661 gctcccgagg agtcataaac tgtcagcact gcggcatatt tctcagtctg ggtgaccact
     3721 gcaactgtgc tgtcaggggc tatctccgcc aaaagtatag actggtctat atcctttgag
     3781 agcagctcgc cctttttatt caacactctg aaagaatttc caccattatc gtaaacaagc
     3841 acacgctttc cggatgttct tacaacaggg tttgcaaagt tgtgagaatt atccgatatc
     3901 ttattgccag actcgtcata aaaagcaatg ttcctgtcgt caactcgaac tataccgtcg
     3961 gaaagctgtt ttataacgtt aacggagctt tcatcaaagg tcagcgggaa atttccgctt
     4021 tgctttttgc cgtcgtttac tatagtggca tgaggtctgt ccaaaatgcc ctcaagcttc
     4081 ggcacccaca ctcctcttga agcgtaaacg ccaactccga aagcgaccac cactgctatg
     4141 gcggctatct tcttatagag ctttttgttt ttctctttct ttcttgccgc tcttatatca
     4201 gtgacgttgg atatctcctc gtcagacctc tctgctttct cggccttttt ctcttttctg
     4261 cttttatgct cggatttttc ctctttgctt tctgaagcct gctctttatt ttcaccgcct
     4321 gatactgtta tcttgctttc atcagctttt tcaggctttt cctcatcggc tttatcctcg
     4381 gtgttcgtca gctctattct gcttcgtgac tcctcggctt cttccgcttc ttttcggagc
     4441 tgttcttcct gctcacgttc cgccgctttc tgagccgcct ttttctgacg ctttatctct
     4501 ctgaaagtaa cgttcggcat aggctcttct gcaccgtgaa actcgtcacg ctgaacaaaa
     4561 tccgtctgct tggttattct tattgttttt tcttctttgg taggcggatt tttctttctc
     4621 cgcctgctct ttgatctttt atcattcacc ttgttcagat cctttatctt tatcataata
     4681 cacttcgtta agatacaatc cctgcggcgg aacggtcttt ccgcctcttg tcctgtcctt
     4741 tgacgcaagt atatcgggta tttgagtttc tcctatcttg ccctcattta taaaaataag
     4801 cgtgcctacc attatcctta ccatattgta cagaaagccg tctcccgaaa ctgtgaatat
     4861 cacaagctcg ccctcacgtc ttacatggaa agagaatata gtcctaactg tattctcctt
     4921 tgtgcaatcg gtggaacaca tggctttgaa atcgtgcgtt cccacaaaat cctgtgcggc
     4981 tttatcaagc tttttttcgt ctattggaaa ccagctgcgg taggctgtat ctttataaaa
     5041 gggatttttt atctcgctgt tatgtataat atactcatac tccttgccgc tgcacatata
     5101 tcttgcgtga aaatcaaggg ggactttttc gcactccagc acggagatat cgtcaggaag
     5161 ctttgaatta agtccgaaaa ctatcccacg gcaggttatt gtggactcta taagaaagct
     5221 tgcacaaaat cttcttgcgt gcactcctgc atctgttcgt gaacagccgt tcaccgtgac
     5281 tgtctgtccc gtcagcgaga ggatagcttc ttcaagcacc tgctgaatag caagggcgtt
     5341 gatctgcctt tgccacccat gataggctgt gccgttatag gcaagtatta tcttgaaatt
     5401 cgtcatgagc atatcccgtt tctttagtcg agtgttatgg aatctatatt gtcaagtctt
     5461 actatcatgg tgctgtcgct tttcttttcg tggacgataa gctttaccca ctcgtcgtct
     5521 gcatcaagaa tagtacagct atggccataa atcccgtcgc cgtcaatggt acattctctg
     5581 ccgataagtt cttttataat gcctgacatt ttacttcccc catttacatt tctctcaagt
     5641 ttcttgactt ttctttccaa agcatcaagt ctgtcatctt ggtccatatc tttaaaaaca
     5701 ctgctcataa tacccctcct aaacgactat atttatgact ataacgcctg caaggaacag
     5761 cacgcagaat atcgagccgt agaggtcgac tatgctcata tggagctgtt tcattcttgt
     5821 tctgccctct ccgccgtgat aacagcggca ctccatagca agggcaagct cctcggcgtg
     5881 tctgaacgat gccacaaaaa gcggtatcag cactgggata agggctttgg ctttctgaat
     5941 gagtgagcct gtttccatat ccgctcctct tgctttctgt gctgacatta tcttgtctgt
     6001 ttcctcgata agtgtaggga taaatctcag ggcaatagtc atcatcattg caagctcatg
     6061 gacaggcact tttatctttt tcagcggtga aagcaatctt tcgatagcgt ctgtcaggtc
     6121 gataggcgag gtggtgtagg taagaagcga agtgccgaca ataaggcaga ttatccttat
     6181 catcatgaac actgccgtgt caacgccttc atcggttatc ttgagaaatt tccactcaaa
     6241 atagg
//
LOCUS       DAWEMR010000149         6209 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_4986_length_6209_cov_9.837829, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000149 DAWEMR010000000
VERSION     DAWEMR010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4986_length_6209_cov_9.837829"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1634 a         1494 c         1181 g         1900 t
ORIGIN      
        1 gatagttctt ttttgctgtg tgatatatta aagcccgtgg gcttccatat tgaggaaatc
       61 cacgggcttt gttgcgcttt ttttacagcg cctgtttttt atattcgaat ctaattactt
      121 cctcttagaa accaccatag ctgcacctgc aagtgctgcc agacctatac ttatgcccat
      181 tgccgcatct gtattagggt ttgtggtgct gttaggattt gtcgttgtct ttgatgatga
      241 agccgctgcg ctgctgtcat ttgttgcttc agaagaaggc tcggaagatg actcctctgc
      301 tttgctctcg ctgctctcct cacttgacga ctcctcaaca acgttattga actcaacatc
      361 gtcgtccttt gtcatttcgt caacgccgtc gttaaccaac tcaactctct cccagccgta
      421 aatgccttca atagcatatg taagctcata agcagctgtc ttctgtgaaa caagctgatg
      481 ggtgatagtc aactgcataa ccattgtttt cgctgtcctg aacgctgaac tggaaaactg
      541 ataccttgct gtcgcctgtt tcagtcttat aagtgtcaac tgcatctgta atagaaggat
      601 aaagctcctg tcccataata ccgagtgtac aaagtatctc agaatttgga ttcttagctc
      661 ttatctcctt gaggaaattt acatagccct tttcaaactc agcacactta tcagggtcac
      721 cctttgtgta tgagctatcg tttgtaccaa gatttacaac gataagctca ggctgatact
      781 gtgagaaatc ccaatcataa ttagctgtct gttcaccgtc gaaccagctc catgtaaagc
      841 agaacttatc atagaaattt ggcacaagca gtgcgtcatt tatcttgccg ttgcctgaat
      901 aaccaaagat cacacctgct ccgcttactg atacaaagct gtagtcagcg ccaaaattct
      961 gtgctgcctt gtaagcatat gtctttgcgg cgttctcgtt gtagatagaa aatgactttc
     1021 caagtggatc gtcaacgccg tatccacaag tgatagagtc accaataaac tcgatagaat
     1081 gctttgctgc ctctgttggc tgtggcttgc cgtctacctc aaagctgtca attgcgataa
     1141 cgctctgtgc agcttctgaa agctttatga gcttaactgt gctttcgccc tcaggaagct
     1201 ctacctcaac tgcgtttgtc ttcttatttt ttatgtagcc ctgcttaaca agcttgccgt
     1261 ttacaaatac gccaattctt gcctcaccta cgctgccgtt tacattgaaa acagcctttg
     1321 tgcctgtgca tttgaactcg ataccgcttg cagaatagcc aagtatcgtt gtgtcgccct
     1381 ttctgtaagt acgaccgata agcttaacat tgtcgcttgt tgcctcataa gaggtgagct
     1441 tgtccgttgc ttccgtgtct gccatgactg tcattcccat gcagcctgct gcaattgccg
     1501 cagcacagaa gcctgccaaa aacttcttca tactatgatt tcctccgttg tttaattttt
     1561 ttgtatcttt tgccttaata tttctcgatc caataattta attatactct atctgcaaga
     1621 tatgtacatt aatttattgt taatatttat tcgtcatttt attaacagga ctgttagata
     1681 ttctttatcc tcttataaac cacagccgac agcggtggac agacgaaact gcctgaaacg
     1741 ggctttttgt tcatcttgcc actttcatca agcctgagat aaaagcttcc ctcaggcaga
     1801 ccgaagcccc tgttctcgta gtaaggatta agtgcgataa cgtagcattc tccgtctttt
     1861 tcaagcttcc agcttacgca gttccaatcg tcattgtgca taaaatgaag atgtgcgttc
     1921 acgtcctctt tagtatgcat tcggaaaagc ggacttccct ttctcagcct tataagagcc
     1981 ttataatact caaagacctc tttatattca agcttcctgt tccactttat ggagttggtg
     2041 tagtcaggcg cattgtaact gtcatgggaa tagatattca catcgtttgg ctcttcgccc
     2101 tctttaagta tcctcggctt tgacctaaga aaatcctgac caagctgtat gaacggcatg
     2161 ccctgtgaaa gtatcactat cgccgccgca agcttatcca tttttatcct tacgtcctcg
     2221 ctgtcattct ttgccgatat acaaagcttg tcccacaaag tattgttgtc atgagcctca
     2281 cagtaattca ccgcctgagt aggttcaaaa gcccagcaag cttcttttgc gtcatttatc
     2341 tgatagtgtg gcacagagcc ggctatacca cgccttaggg tagaagccgc atttctgttt
     2401 ccgctgacaa agcccttgtc atatggattg aaagtaccgc ccttaatgga atctctgatg
     2461 ttatcgttga aaagtccaac tcttccaaac tgatatgagt tccatttata tgcaagcctg
     2521 tcagcaggga gcggtgactc tcctcccgtc cagccctcgc catacattag tatctgagga
     2581 tctatcttgt taagctcgtc ccttatgatg ttcacagttt caatatcgtg aagtgccata
     2641 aggtcaaagc ggaagccgtc tatcttatat tccgttgccc aaaatctgag agaatctatc
     2701 atgaattttc tcatcatcat gtggtttgaa gccgtttcgt ttccacagcc cgagccgttt
     2761 gaaaaagagc cgtcatttct tatcctatga taataatatg gaaaagaacg ctcaaaggct
     2821 gactcctcgg tatagtaagt gtgattgtat accacgtcca ttacaacgcc aatgctgttc
     2881 tcgtgaagtg ccataacaag ctgtttgaac tctcttatcc tgcacttagg gtcataaggg
     2941 tctgtagaat atgacccctc aaggcagttg aagttcttag ggtcataacc ccagttatat
     3001 tgattgagcc aaggcttgct ctcgtccaca gtggcataat cttccacagg gagcaggtga
     3061 acatgggtta tgccaagctc tttgagatgc tcgatacagg tgttcacgcc ctgatatttc
     3121 gtacccttat cggcaaaggc cacatacttt cctctatgct caggagcaac gcctgacgaa
     3181 ctgtccgcag aaaaatccct gacgtggcac tcatatacca ctgcgtcgca ggcatttctg
     3241 cacttaggct ttttcacctt gtcccagccc tcagggtcgg tggtcttgaa atttaccacc
     3301 gcaccaacgt ttccgttgac tccgcaggct tttgcataga tatctatagt ttcatgcctt
     3361 gttttgtggt caaactcaaa tgtataggta taaaaaacac cgtctaggtc acggagtatc
     3421 tccacatacc aaacaccgtg gtcaagcctt tccataggca ggctttcaag acggcttttg
     3481 ccctcaccgt caagatacag attaagataa actcccgttg caagcggcga ccatgttttg
     3541 aacacagttt tgccctcttt catgatcgca cccagatcgt ttcctatata ttgattatct
     3601 ctgtcgaact gttcataatt caatctgtac attaaccatg ccccttgcct atatttttta
     3661 cgtcttagat atttttactt cagactttcg ccaagcaccg agccttgaag aagcttagtt
     3721 tcaaagccct ctctcgtatt tttaagcagg tcatcgcaaa gctttacctt gtccttttca
     3781 agaagcagca ggatagttga gccgccaaac tcgaaatagc ccttttcttc gccctttgtg
     3841 acctttacgc cgccctcagt gtgcagatta gttatcctgc ccaccataag agcaccgact
     3901 tccatttgaa taatatctcc gaactgctcc gttcttatca tgcaatattc tctggaattt
     3961 tccttgaaaa caggcagata attgttcacc acagggttca cagtgtgaag aataccattt
     4021 atctttctgt tgtggctctt tacaccgtct gcacaatagc aatagcggtg ataatcgtcc
     4081 actgaaagtc tgatgataac tgcatagcca ccctcataac gctttgcaag cttgctgtcc
     4141 ctgagaagcg aggcagtatt atacactgcg ttctttataa taaaggtatc agactgtgtt
     4201 atcttataag cagtcacctt gccgtctgaa ggtgacacca gcagattctt gtccttagtg
     4261 ataaaacgtc tgccaggctt tatttttctt gtgaaaaaat cattgaatga gcgatacatt
     4321 ctttcctcat attcaaacat atctattccg tttttttctg caaagccgaa aataaaaggc
     4381 actgagaact tgctgtcaag tatcactctt gcacactttg aaaatatcgg acagccgaga
     4441 aatttcataa gcgccctgcc cattgccgag ccgtaggcag ccgcaagaaa gctatcctga
     4501 gatgtggtct gttcatattc tctgccctgt ctgtcacgat atttcatttg tttctctcct
     4561 tttatttaca ttattgtagg ggacgacacc cccgacgtcc tgtttttcac acacctgtcc
     4621 ggcaaaaaca ttttccattc aggtaacttt taccttagat tatatgctgc attttaagat
     4681 agccgagaag gattatcgca cctatagtaa agagtatcag cacgcctttc atggaaagct
     4741 tcttgacatt tattttcaat acaaagagta ttgaaataag tatcataaag ctatggaaaa
     4801 caaatggaaa aatatttctc ggtatattgt gctgacccat gaacagcaga ggaagtatca
     4861 tagcaactgt tgtaacaggc aggcccttat attcttttct gttctctgtt gtgtttctct
     4921 gacgctcttc ctccataacg ttgaagtagc caagtctgat aacagcgccc agaactatca
     4981 taatgcttgc gataagacga agcggataaa ctgaattgta gtgatagcct atgacagcag
     5041 ggaaaacacc gaagcaaaca aggtcgcaca gtgagtcgat ctgtatgccg aacacctttt
     5101 catgctctgt tctgtttttc atgcttcttg ctatcttgcc gtcaaaaagg tcgcagatac
     5161 ctgaaagaag caggcagaga aaggctatgg acatatgatt ttcaaagacc tgagtgatcc
     5221 cgaacactga cgaaacaagt cctatgtatg tgagtattac cgaataatta taaaaaccga
     5281 tcattttttt cttttcctct cattcttttt cgacttcttt tttaacttcc tgcgacccct
     5341 caagaccatt cttcttatca ttcctcataa cgataaagtg agccacgaat gttaccaatg
     5401 cgctcattat cagcagtaaa tagaagctga gtccacggct cagtagcatt gcaggcttta
     5461 caagctctgc ggtgaaaatg ctcatgaaca tatatgtgaa gcagccctct gttacgcctg
     5521 ccgcaccagg caatggcagc atttcaactg caacgcctat catagtctga agtgcgataa
     5581 tattccaaaa acttgttccg ctaaggccgt aggacttata cactatccat gtgacagcca
     5641 gcaggaacag tctctgcacc agtgtgataa gaaaaatatt tatcacagta tgcacattgc
     5701 tctttatata ttccgaaccg atcatgtaat tgtcgcagat acgctttatt ttgtttatgt
     5761 atttttcgtt gttcttttct ttgagtatcc ttatgcgtgt cagaagattt ataagcttta
     5821 tgcccatttt tctcgcccac aaaggcttat agaaaataaa cacaagccct gcgatatagg
     5881 cgatattcag tataaatccg ataagcagaa gccaccaaag ccgtcccaca tgaagcttta
     5941 taaagccaaa gttgaaaagc aggaacgcca ctgcaagaag cacctgcaca gccttgaaag
     6001 ctatggtgat gataagcatt atcaaggtgg aaaagcctat ctttatgcca tctttcttca
     6061 tgtaatacat ctgcgcaggc tgtccgccgg aagcagacgg cgttatacag ctaaagaaaa
     6121 agcctataaa cgaatatttc aaacaacgcc tgaaaggagt ttttacttca aaaagcctca
     6181 gcatatactt tattatgacc gactcccct
//
LOCUS       DAWEMR010000150         6162 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5028_length_6162_cov_9.411495, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000150 DAWEMR010000000
VERSION     DAWEMR010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5028_length_6162_cov_9.411495"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    383..392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2188 a         1071 c         1214 g         1679 t
ORIGIN      
        1 ctattttgat aacctgtttt taaaaaacaa agttaataaa attataaaag acatttccga
       61 aaatatgcca gttataattg taacacataa cagcacagtt ggcgcatcaa ttaaacctga
      121 ttttttgatt catactaaac gttttattac agatgaaaaa gaagctattt ataaagtgta
      181 ctatggacat cctaacgata aagaattgca tacaattcac ggagaagtaa tcaataatgc
      241 aaatgtcaca atggattgtc tagaagccgg agaagatgca tataatgaga ggagaagtaa
      301 ttatgattta cttaaaaatt gaacgatcgc aagcctcata tatagatgag aatggtaata
      361 tatgtctgat aacaaatatt gannnnnnnn nntcctgaga aaaacaagat tcccaatgaa
      421 gcacaaaaaa taatatacga aaatatctat aataagctaa atgacttgat agcgaataaa
      481 gaaaaaattc tcagtgaaat atccaatttg tataaagaag aatatagcaa atattctata
      541 ccaaatatag cagatggtga tagtgatgaa aaatgacgca tacctcgaag ataacatgag
      601 caaataccct gcgatcgaat tgctgaaaaa gctggagtat acatacatat cccccgaaga
      661 gtgcgaggct cagcgtggcg gactttacaa cgtcattctc aaagatatcc tccgctcaca
      721 gcttgaaaaa ctgaacagct atactttcgg cggtaaggag tataaatttt caaatgagaa
      781 catacagcga gcagttgatg agatagacga gccgctggca gataatcttg ttgtgtccag
      841 tgaaaaggtc tacaatgata tcatgctcgg acgaagctat cccgaaactc tccccgacgg
      901 cagaactatg aatttcaatc tgaaatacat cgactgggac gatattacaa acaacgtctt
      961 tcatgttacc gaggagttta ctgttcacag cgcagaccgt cagcatgata cccgtcccga
     1021 cattgttctc tttgtcaacg gtattccatt tgcagtgata gagtgcaaag ctccgacaat
     1081 ctccgtagaa caggcagttg agcagactat ccgcaatcag aaacaggaat acactccgca
     1141 gcttttcaag tttgctcaga tagtcgccgc aacaaataaa aacttcttca aatatgcgac
     1201 cacaggaact gcgaagaagt tctggaacgt atggcgtgaa gaagatgccg agtggcttga
     1261 cggactgctt tcacagcata tcaccgaccg cagtatcact caacaggaca gggatatcgt
     1321 gtcgctcttt gacaggcaca ggcttatgga gcttacaaag tatttcatag tctttgatgc
     1381 caacataaag aagatatgcc gacatcagca gtttttcgct atccgaaaga taataaagac
     1441 tatcaacacc gacgatgaga acggcaatcg tcagagtggt gttatatggc acactcaggg
     1501 cagcggaaag tcccttacaa tggtaatgct tgcgaaatat atcctcatgg aacttacaag
     1561 ctgtcacccg aaagttatca tagttaccga ccgtaaagat cttgataacc agattgctaa
     1621 gacatttgca cacaccagac tttcaccggc aaaagccaca tccggaaagc atcttgtcga
     1681 gctgatacag agcgataaag ctgatgttgt gacaacgata atcaataaat tcaatactgc
     1741 cgaaaatcag aaaatcaaga taaatgaccg caatatattt gtgcttgtag atgaaagtca
     1801 ccgcagtaac tatggaacac ttgcgactaa gatgcgaata gttttccctt gtggatgtta
     1861 cataggcttt acaggtactc cgctgatgaa aaaggagaaa tccactgtcg gacgtttcgg
     1921 cggcggcgac tatatccata agtatactat caaggatgca gttgatgata aggcgatagt
     1981 tccgctcatt tatgagggac gtttcgtaga tcagaaggtc gacgaagata atatagacct
     2041 gtggtttgag aagactactc gccgtcttaa cgagcgtgag cgtgaggatc tgaagcagaa
     2101 atggagcagc atcaagcgcc tgacttctac cgatgcgaga atccgcagga tagctcttga
     2161 tattgaagaa cactttacag acagcgtaaa gaatacaggt atgaaagcga tgcttgcaac
     2221 aaatttcaag cgtgacgctg tacgttatct tgaagtattt gagcagcttg gaagtcttaa
     2281 ctgtgctgtt gttatatcag ctcccgatat gcgtgagggc gaagacgata tactgtcaga
     2341 gacagataac aaagtcatag cgttctggaa taagatgatg aaacagtatg gcgatgctga
     2401 tacctatgaa gaaacaatca agaataaatt ctgcgacggt gacattgata tccttattgt
     2461 ttgcagtaaa ctgcttaccg gctttgatgc accagtgtgt caggttttgt acattgataa
     2521 ggaactgaaa gaacatggct tgcttcaggc tattgcccgt acaaacagac tgtatgacgg
     2581 caaggattat ggcctcattg ttgactatcg aggacttatt cagaaactcg atactgctat
     2641 gaatatgtac agtggtgcag gtcttgaaaa ctatgaaggc actgatcttt caggagctat
     2701 cgttgacgtt atgacagttg taggagaact tcgagaagca tattctcagc tctgtgaact
     2761 cttcataggc atcagaaaca aaaatgatgt aaatgaatat gaggacaagc tggaagatga
     2821 agatcttcgt aatgacttct ataatctact ctgtaaattc ggaaaagctc ttgctatcgt
     2881 agttaattcc gagaaggctt atgaagctgt tccccgtgag gagataaata agtataaatc
     2941 cgaattcatc atgttctcta agatcagaaa gtctgtaaag ctccgctatg ccgatgctat
     3001 cgacaataag gaatatgagc ctataatgca gaatttgctt gatcagaatc tttcagtaac
     3061 aggcttaaaa atcgttacag ctccggttga tattctggat tcagatggac ttgaaaagga
     3121 actgaacgaa cttggctcag caagatcaaa ggcagatgct attcgttcaa gaatgactaa
     3181 aagcatatcc gaaaaatatg acgaaaaccc tgcttattat gagaatttct caaagaaaat
     3241 aaaagaagct cttgaactgt ataaaaagca ggtaataagt gaagcagaat atcttgaaaa
     3301 gatgactaag atcatggagg agtaccgcag cggaaaaact gaactcaagt atcctgaaag
     3361 aattaaagac aatgttcatg ctcaggcatt ttatggagtt gtttcagcta tacttgatga
     3421 agtaataccg ctttcagaaa acagagatat agtagctgat ctgtcggcgg atataacaga
     3481 catcataaag gaatacagta aagtagactg gacttcaaac attgacattc ataagaagat
     3541 atctcatgca ctggacgatc ttctctacga ttacagcgac aataataact ggaatctcag
     3601 ctttgagatt atggataaga tcattgacaa tgttaaaacc attgcgctca ggaggtttta
     3661 atgaaagtac tgtcaacaga aacaagaaaa gttaaaactg agtgcggaga tattgaatac
     3721 atttttgagc gcaagaatat caaaaacata aatctcagaa tacgtcataa cggaagtatt
     3781 tttgtttcag tgcctgagaa tgttgctgag aatatagcag attcttttgt tatcagtaag
     3841 tgcaggtata tcagaaatgc tgttgatact tttgaaagca atgagatact taaagctgaa
     3901 atgaagtatg tcagcggaga aaatgttaca cttctcggaa aaaatatgcg gataagcatt
     3961 atcaaagatg cagaggagta tgtaaatacc gacggcattt atgtttgcat ccatgtaaga
     4021 cgacccgact attataacag aaaaaagaat cttctcaaca gctggctgaa tgaacagtgc
     4081 agtatcatct tcaatgaaat aatgcatgaa gttcatcaga aattcattcc gtatggagtt
     4141 gattttcctc agttgaaacc tcgtaatatg acttcccgtt ggggcagctg tctgccgaat
     4201 aaaggtataa taacgcttaa taaacgattg atcgaagctc ctaagaactg cattgaatat
     4261 gtggtgtatc atgagttttg tcattttatt catcctaatc attcaaaaca gttctactct
     4321 cttttacagg taatgctgcc tgattggaga gagagtaaac agcttcttga aaattgcatt
     4381 gttttataga ggtggtattt atgaagttta caattaagga aatacgcttt aaagattata
     4441 aatcgtttaa aggtgattat atatcattaa agccagaaaa attcacactt cttattggac
     4501 gcaacaactg tggaaagtca agtataatag atgtatttga atcaataaca tccccttctt
     4561 ttttccataa acaccgttgt tctattctaa ttgatattaa cggacaaaag gtgagaaatg
     4621 aaccgttctc agggtataaa gaatatatag gcgataaaac atatagaatt gagcctatga
     4681 ataatacttt tagatgcaat ttattagaag gtaattatca aacgagcatt attgacgccg
     4741 agccccatat tagtaatttc ctacaacaaa tctctttcag aaggctcaat gcagatcgtg
     4801 atattattcc agaaggctat aattcagatt atcatttaga tgaaaatggc aacggagctt
     4861 gtaacctgat caatcaaata ctcaccaaag aacaattacc tgaaaaactg atacaaaaaa
     4921 acttgcttga agctttaaat agaataatgt gtccggattc aaaatttgat ggtatcacag
     4981 tacagaaaac agctgaaagt gataattggg aagtctattt atacgaaggt gatgaaagat
     5041 atgccctctc taaaatgggc agtggattaa aaactataat acttgttctt ttaaatctaa
     5101 taatcatccc taaagttgag aaatatgatg atgaaaaaat gtgctttttc gcatttgaag
     5161 aacttgaaaa caatctgcat ccggcattgc aaagacgtct ttttgactat cttttccaat
     5221 acggaaaaga tcatctcaat gtatatatat tcttaacaac acattcacat attgcgatta
     5281 atacatttgc aaataaacca gatacacaga tacttcatgt caaaaaagaa aacggtattt
     5341 ctactatgga tacaatagac agctttattt caaaaactca tgtattagaa gatctggatg
     5401 ttagagcaag tgatctattt caatcaaatg gaataatctg gattgaaggt ccatcggaca
     5461 gaatatatat caaaagatgg ttagaaatat ggggagataa agaactcatt gagggttcag
     5521 attatcaatt cctatattat ggtggaagat tgctttcaca ttatactatt gaaggaataa
     5581 aagatctgat aaacattctg tccactaata gaaatgccgt tattgtaatt gatagtgata
     5641 aaagatcaaa acagggacat atcaacgata ccaaaaaacg aataaaaaaa gaatttgaag
     5701 aaaaaggaat gctctgctgg ataacaaaag gaaaggaaat tgaaaactat cttccattta
     5761 gtgctattaa ttcggcatat tcttcaaaac tcaatgcaaa ttgcacacaa tatgaactct
     5821 tcccagaata tattgatgat gtatgcaagc attttactaa taaaaaagtg gaatttgcaa
     5881 agaaagtcgc accatatata tctgataatg atgcaagcaa tattttaggt gtaaaagaaa
     5941 tgattatagc attaaccagg agaataaaaa aatggaattc aagagataca tcataaatca
     6001 ctaatatata cgtctttgtt tttttctgat gatcatactt aatgccctcc acgggcagtg
     6061 gcgctgacgt gcagtccgat tgcatcgagc aatgtccctg ttcctcatcc aagattcgtc
     6121 acagagccac tctctaacga gagaaatcaa aaataaaatc tc
//
LOCUS       DAWEMR010000151         6130 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5063_length_6130_cov_14.350453, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000151 DAWEMR010000000
VERSION     DAWEMR010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5063_length_6130_cov_14.350453"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1627 a         1402 c         1245 g         1856 t
ORIGIN      
        1 gacaactgcg gctatactga aaacttatct tatcaaaaag gatatgtagt ccataatatt
       61 ttaaccatgc gataaaagaa acggtcgaac gctgttcgcc actgcataca taaagggtac
      121 tgtcaaagtg gcagtaccct ttttttgtgg taaaaaaaag agcgcccacg caccgaagtg
      181 catgagcgtc ttttttatag taacttagat tacttttcct cagtattgta aagcttagcg
      241 tatctgagca gataatcata tctgtcctta gcattctgaa cagccttacc aaagagcttc
      301 tcagctctct caggattctg tctagcaagt gcgttgtaac gtacctcgcc catgatgaag
      361 tccttatagt cagctgttgg agccttagaa tcaaggataa atggattctt gccctctgcc
      421 ttaagacgtg ggtcgaatct gtacagatgc cagtaaccag cctgaacagc cttcttctcc
      481 tctgtctgag caattgacat accacccttg ataccatggt tgatacatgg agcataaccg
      541 atgatgattg aaggaccgtg ataagactca gcctcgtgga tagccttgat gcactgattg
      601 taatcagcac ccattgcaac gtgagctaca tatacatagc cgtaagacat tgcgatacct
      661 gcgaggtcct tcttcttaac ttccttacca gctgctgcga actgagcgat agcacctgtt
      721 ggagttgact tagatgactg tccgcctgta tttgagtaaa cctcagtatc gaatacgaag
      781 atgtttacgt cctcgccctg agcgataacg tggtcaacgc ctgagaatcc gatatcgtaa
      841 gcccagccgt cgccgccgaa gatccacatt gacttctttc tcagatagtc agcgtcctta
      901 aggatctcct cagtagcctt attctttgtc ttcttaagag cagcgataag gttatctgta
      961 gctgctgagt tcttctcgcc gtcgtctact gtttcaagat actcttcgca agccttcttg
     1021 agagctgcag ccttagttgt cttagacagc tcaagaacct tagcgatagt tctgtttctg
     1081 atagttgact gtgcaaggaa cataccgtaa ccatactcag cgttatcctc gaacagtgag
     1141 ttagcccaag ctggaccctt gccgtccttg tttactgtgt atggagttga aggagctgaa
     1201 ccaccccaga ttgatgaaca gcctgttgcg ttagcaatgt acattctgtc accaaagagc
     1261 tgtgtgatga gcttagcata aggtgtttca ccgcagcctg cgcaagcgcc tgagaattca
     1321 agcagtggct gcttgaactg tgagccctta actgttgttg ccttgaactt ctcgaatact
     1381 tctggctttt cagcaagtgt aagaccatag ttgaatactt cctgctcagc gagctgagtt
     1441 tcaagtggca tcatgctgag agccttgttg cccttcttac caggacaaac atttacgcaa
     1501 gaaccgcagc ctgtgcagtc cagagcagac attgtcatta cgaagttgta accaggcata
     1561 cctgtcatag ctactgcctt ctcctgagca agcttaggag ccttcttcag ctcagcgtct
     1621 gtcattacaa caggtctgat aactgcgtgt ggacatacat atgaacagaa gttacactgg
     1681 atacagttgt caccattcca tgcaggaacg tcaacagcga tacctctctt ctcaaatgca
     1741 gcagaaccct gtgggaatgt gccgtctgcc atacctgtga atgttgaaac agggagcttg
     1801 tcgccctcct gagcattgca agggatctga atgttgttta cgaagtcctg aagtgtctta
     1861 tctgcgcact tagcggcagg catacccatt ggagtatcct tgcacttctt ccaagatgca
     1921 ggaaccttaa cttcgtgaac ctgctgacat ccagcgtcga tagcgtcatg gttcatctta
     1981 acgatagcgt cgcccttctt agaataagaa gctgtagcag cgtccttcat gtacttgata
     2041 gcgtcgtcga taggaatgat gttagcaagc ttgaagaatg cagactgaag aacagtgttt
     2101 attctgttgc ccagaccgat ctccttaccg atcttaacgc cgttgatcgt atagaacttg
     2161 atgttgttct cagcgatata tctctttacc tgacctggga ggtgcttatc cagctcttca
     2221 tgctcccact ggcagttcag aaggaatgtt ccgcctggct tgatatcctg taccatgttg
     2281 tacttagtga tataagactc gttatgacaa gcaacaaagt cagcctgatt gataaggtat
     2341 gttgacttga taggtgtctt accgaatctc aggtgagaaa ctgttacacc gcctgacttc
     2401 tttgagtcat atgcaaagta tgcctgagca tacagatctg tatggtcacc aatgatcttg
     2461 atagagttct tgtttgcacc aacagtaccg tcagcaccaa gaccccagaa cttacaagca
     2521 gttgtgcctg ctggagcaga gtcaagcttg ccctcagttt cgagagagag gtttgtaaca
     2581 tcgtcttcga taccgatagt aaatcttggc ttatatgtat ccttccagct tgcattccag
     2641 taaacagcct tgatctgtgc aggagttgta tccttagaac caagaccata tctgcctgaa
     2701 gcgatagcta catcgtggaa ctgtgtgccc ttaagagcag caacaacgtc aagataaagt
     2761 ggctcgccct cagaacctgg ctccttagtt ctgtcaagaa ctgagatctg ctcacaagtt
     2821 gaagggatag cttcaacgag cttgtcagca cggaaaggtc tgtaaagtct aaccttaaca
     2881 agaccgagct ttgtgccttc cttctcgttg aggtaatcaa ttgtttcttc gatagtgtca
     2941 caaacagaac ccatagctac gataacgtgt gtagcgtctg gtgcgccgta gtagttgaac
     3001 agcttgtagt ttgtgccgat cttttcgttt accttgttca tgtaatcctc tacgatgcca
     3061 gggatagcgt catagtatgg gttacaagct tctcttgcct ggaagaagat atcagggttc
     3121 tgagctgtac ctctaagtac aggatgctca gggttaagag ctctctttct gaactcctca
     3181 accttctcga agtcaaccat atctcttacg tcttccatat cccatgtctc gatcttctgg
     3241 atctcgtgag atgttctgaa tccgtcaaag aagtggatga atggtactct gccctcgata
     3301 gttgagaggt gagcaactgt acccaggtcc attacctcct gtacatttga agaacaaagc
     3361 atagcaaaac ctgtctgacg gcaagcatag atgtcagaat ggtcgccgaa aatattaagt
     3421 gcgtgtgaag ctacacatct tgcagataca tggataacgc aagggagaag ctcacctgcg
     3481 atcttataca ggttagggat cataagaaga agtccctgtg atgctgtata tgttgttgtg
     3541 agcgcaccgg ctgcaagtga gccgtgtact gtacctgaag cacctgcctc agactgcatt
     3601 tctgcaagct ttacaggagt gccaaacagg ttttccttac ccttggcagc ccactggtct
     3661 gttacttctg ccataacaga tgatggtgtg attggataga tagcagcaac ttctgtaaat
     3721 gggtatgacg cccaagcagc agcgttgttt ccgtccatgg ttttcatttt tcttgccatt
     3781 ttagaatcat tcctttctcg atatttcaag caaaagctca aaactcaaat ttccttttta
     3841 gcgtactttt tcaagcacgt ctgtttaagg acagcagata ttttcttcaa aagtaagcta
     3901 taacaaactt tctcaggata catctgtttt aactcttata gtataccact tatttttcaa
     3961 tttgtaaaga ccttttacca cattttttct tgatttatac gctgtaaacc ccctattttc
     4021 ggttagttat gacttcctca tatttcagac gtgctgtatt acggagataa aaaaaatgcc
     4081 tgccgaaaca gacatttttt atatatataa attataaaac tagcagttca taagaagttc
     4141 ggcaagggca ttgagtatct tatccacatc aacatcagag ctgaatttta catcaagctt
     4201 tgaaccgcct tttttatcct cactgagact tacacggagt ttatcggtag cctgatttac
     4261 tcttatctca aactcagtat acatgggatt tgtgatagta cgctttattt tcttttcagc
     4321 ttttttcttt gtttcaaaag cggcgatagc cttttctagc tctcttaccg tccagctctt
     4381 ttctatgcac tcggcagcaa gcttttcctg agtatcctta tcagcaacgc ctgcaagcac
     4441 ctttgcatga ccctcagtta gctttttgtc acgaagcatt tcctgcacat tttcgctaag
     4501 tgaaagaagt ctgacagagt ttgccacagc ggaacgtgaa cgtcctacag cttctgccac
     4561 ctgctgctgg gtaagaccat aggtttccat aagagtctga taagcggcgg cctcctcaag
     4621 aggtgtaagg tctttacgct ggatattttc aataagggat atctcagcgg cttcaagatc
     4681 ggtaagttcc tttatatata caggtatctc ttcaagccct gccagacgtg ccgcacgcca
     4741 cctgcgctcg cctgcaacta tcttatatcc cccattgtca agaggtctta caagtatagg
     4801 ctgtaaaacg ccatgctgtc ggatattttc cgcaagctca ttaagagcgt cctcgtcgaa
     4861 ttcggaacga ggttgttttt tgtcaggttc aataagagat attctcactg tggagatacc
     4921 attctcaccg ccgtcatttt caaccgcact gctgtcggct tctgaaattt catctataga
     4981 attatcaaaa aacaggctat ccaagcctct gtccattttg ctttttttag ccattgtatt
     5041 ttcctttcac attataacat acaatgttcc atgtggaaca ttatttttct ttattctttg
     5101 ccgctttttt cttttctctt ttagctattt cttttgcaag attttcatat gcctttgagc
     5161 cttttgcctt cttatcataa taaatagccg gctcaccaaa gcttggggct tccgacaagg
     5221 caacgtttct cggtatggta gtttcaaaga cttcctttgc aaagtgcttt ttgacctcgc
     5281 ttattatctg acctgttatc ttatatcttt caacgcacat tgtaaacaaa atgccctcaa
     5341 tatcaaggtc aggattgaaa gaaactttta ctctgttgat agtattgtga agctcaacaa
     5401 gaccttccaa agaaagaaac tcgcattgga gagggataag tacagaatca gccgccgcaa
     5461 gtgcatttat tgtgagcata tcgagagtcg ggggacaatc tataaatatg tagtcataaa
     5521 attcttttgc aggcataaga gcattgcgaa gcttcattgc tctgtccttt tcattcacaa
     5581 gtgacacaga tgcacctgaa agatcctgag ttgtcggcac aactgaaact ccacggcagg
     5641 aagttggcac gacagcttca aatatcgaac attcttcaaa tattacatca aacactgtat
     5701 ttctgatact ttttttcttt ataccaaagc ttgtggtagt attgccctgt gggtcaaagt
     5761 caacgatcag gacttttttg ccctttagtc caaaaaccgc tgcaagattt acaacagtag
     5821 tagattttcc aacgccgcct ttctgatttg aaacggcaat tattttcccc atattcttga
     5881 cctcttttct tagtagaatt atatcatatt tattatacta caatttttca cttttgaaaa
     5941 gaccctttta caaattttct tgaaaatgtt ccacatggaa caaatcaggt ttttacttta
     6001 tccgacaaag cccatgacaa tttagaaaat aatataaaga ataaagtacc aatatcaaaa
     6061 tcaaggtcat aaagagcaga aaagttttct gaataaaata tagaattgag gatcgtgaag
     6121 tgttatgatt
//
LOCUS       DAWEMR010000152         6123 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5073_length_6123_cov_8.651285, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000152 DAWEMR010000000
VERSION     DAWEMR010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5073_length_6123_cov_8.651285"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4137..4148
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1731 a         1362 c         1151 g         1867 t
ORIGIN      
        1 tatgacagag acaagtatgg ttttgacgga cacacctgtt cagttctcac aactatagac
       61 aagcagtcgc ctgatattgc aatgggtgtt gacaacgctc tcgaaggcag agctgaaaac
      121 gcattcgata caggtgcagg cgatcagggt atcatgttcg gttttgcttg cgacgaaaca
      181 cctgaactta tgccaatgcc tatatcactt gctcacaagc ttgctcttaa acttacagaa
      241 gttagaaaaa acggcacttt gccatatctc agaccggacg gaaaaactca ggtaacagtt
      301 gaatatgatg atggcaagcc aacaagagtt gacgctgttg tagtttcttc acagcatgat
      361 gacgctgtaa cactggagca gataagagaa gatattatcg agaacgttat aaagacagtt
      421 atccctgctg aacttcttga tgagaacact acttattttg taaacccaac aggaagattt
      481 gtagtcggcg gccctcaggg cgacagtggt ctgacaggaa gaaagataat cgttgacaca
      541 tacggtggat atgctcgtca tggtggtggt gctttctctg gaaaagatcc tacaaaggtt
      601 gacagaagcg caacatatgc tgcaagatac gttgcaaaga atatcgttgc agcaggtctt
      661 gccaagaagt gcgaggttga gcttgcgtac gctatcggtg ttgctcaccc tgtttcaatc
      721 atggtcgaca catacggcac aggaaaggtt tctgatgaaa agatagaggc agctgtagaa
      781 aaggtatttg accttcgccc tgctgcaata atcaagagcc ttgaccttag aaagccacag
      841 tacaagcagc ttgcagctta tggtcatatg ggacgtgagg atcttgatgt agcttgggaa
      901 aagacagaca agatcaatga tcttcttgaa gcagttaaat aataaatatc aaagccttga
      961 caggaatgtg cctgccaagg cttttttgtg ttcagcatta attttgtttt agtctttgcc
     1021 tttaagaata ggcgacaaag gcttataaat aagaattgtg accaatgcac aaataatacc
     1081 cttcacaaaa gtaaaaggca gattgaaaat acacagtgca gagaaaatat tctttacgct
     1141 gcccattatc tgctggtaca taccgagtat agcagcttct ccgccgaatg ctttaaagta
     1201 tataggataa acgataaaat aattcaacgg caagcttatt actgtgaaaa ccaaagtgcc
     1261 aatgatacag cttatgatag cattctttct ggtttttttc ttgtggtata taacagaagc
     1321 cgtcagcaca aaagctgcgc ttgttacaaa gtcaaagatg ttgccgatac ccattgttgt
     1381 gcctagtccc tttataatca actggataac gttcttgata aggcaaacga tcacgcctgc
     1441 aataggtccc attgagaaac ctgccagcag ggatataacg tttgaaaggt caagcttgat
     1501 aaatcctggc ataagtggca cagggaaatc aaggtaatat attacagtag ctatggcgct
     1561 gagcattgcc accgttacca tttttcttgt tgtaaaagtt ttatttcttg gcatagtgtt
     1621 atcacttgtc attttaattc ctccaaataa taaaattatc gggagaaaca gcagcaaaag
     1681 caaaagcacc ctgaaaataa aatcagggtg caatttatgc gttattgatc tgtcgtttct
     1741 tccatccgga ctttaccgtc ggtttcggat tttcaccgaa tcagcgcatt tctgcggtcg
     1801 aggacttacg aatactattc gatcacctcc ggtgtggaat ttcaccaccc ctgaaacaca
     1861 ttattaaatt ttgctgaaca ctttttatga gatcaatcct cgttgtcctc gtcctcaatt
     1921 tctgagaaat caaactcaag gctctctcca cagtttggac agtcaacaga accgtcagcg
     1981 ataatgttct cgccaagcat tattgtgtca ccacagttag ggcacacaca ctcgtacata
     2041 gcgtcatcat catcatcatc atcgtcatca tcacaacaac aatcatcgtc gcaacaacaa
     2101 tcgtcgtcat catcatcacc ataaattaga tcttctatct cgccgagatc ctcgtcaaga
     2161 gtatctacaa gctcaactgt ttcgtcaact tcttctgcaa tatcctcaac tgtagcagac
     2221 atctcatcaa gaatatcagc cataattgtc aatatctttc cctcgtttgt ggaatcgtca
     2281 atcttaagac cttccataag tccctttaaa tatgcaacct tctttgacag ctccataata
     2341 ttccctccgt ttctaaaaaa aaccgtaaag gctaaaactt atgctctttc catgtactca
     2401 catgtacgag tatcgattct tattctgtcg cctgtgttga tgaacagagg aactctgatc
     2461 tcagcaccag tttcaactgt tgcaggcttt gtagcatttg tagctgtatc gcccttgata
     2521 ccaggctctg tctctgttac ttcaagatca acaaagtatg gaggctctac gccgaatact
     2581 gtacctttgt aagaaagaat cttacatacc atgttttcct ttacgaactt aaagttatct
     2641 gaaagctcag ccttgtttac tggaacaagc tcatagctct ctggatccat gaaataataa
     2701 agatcaccgt cattgtaaga atactccata tcctttctct caacatatgc tgttgggaat
     2761 ttagctgttg gattgtatgt cttttcaaca acagaacctg aaattacatt tctaaccttt
     2821 gctctgacga acgctgcacc cttaccaggt ttaacgtgct gaaactcaac gatctgcatt
     2881 acgttgccgt cctcttcaaa agtcacgccg tttctgaaat caccagcagt taccattatt
     2941 tagtacctcc ataaacattc ttaattaatg tgtacacagt tcaaaatgtc cgcatacctc
     3001 tatatttatt ttaccatatt tatttgttta ttgcaatagc ttttgaaaac ttttttacca
     3061 aaaaatcaaa aaaagttaca agcaaataag ctctttagag gcttttgtca tattgataca
     3121 gccgttttct gtaacaacaa caaaatcttc tattctcaca ccaaacttgc cctcaagata
     3181 aatacctggc tctactgtta cgatcgaatt ttcaggcaat atatcttccc tgctaggcga
     3241 agaataaggg ctttcatgta tctcaactcc cacaccatgt ccgagagaat ggccgaaatt
     3301 atctccatag cctgcttcag ttatgatatc ccttgctatt ttgtcaagtg cttttcctga
     3361 catacctgcc tttactgcgt caagagctgc aagctgtgca gtaagaacag tttcgtatac
     3421 aaatttcatc ttgtcactag gtttgcctac gcatacagtt ctagtcatat cgctgtgcca
     3481 gccatcataa gttgctccga aatccataag cacgaactca ccgctctcaa cttttttgtc
     3541 agatggtaca ccatgtggaa gcgaagtatt agagcctgaa agtgcaatag tgtcaaaaga
     3601 cagagcttca gcaccatttt taagcatata atagtcaagc tccagctgaa tttctttttc
     3661 agttctgccg accttgataa agtcaagcat atgtgcaaaa cccttttcag cgatacgctg
     3721 tgctttgatt atcttgtcta tctgctcttg ggatttaact gttctgatct ctcgtattat
     3781 ttccgcaagc tttgtgtctg cgatgacctc agcattaagc ttttgctgat atgcgataag
     3841 ctcggaaagt gtgcaggtat cggcattaac agaaactgtt tttgcaccat gcttttcaat
     3901 aaggctgttt atctgctcat agagcttgtc ctgcaaaata acctcgcaag ctgtgacagt
     3961 tttctttgcc ttttcaatat atctgaaatc tatgataaaa tacgcttttt ccggaaaaac
     4021 cactagtgtt cctgcacttg atttcattgc ggtgaaatat cttctgttca catcgtcagt
     4081 gataacagca caatctgccg cttctgttac tcgtgaacaa agtttttcaa gatcagnnnn
     4141 nnnnnnnntg caccgctttc atttttccat ttgtaataca aagcgtcaac agccatgtag
     4201 tatgaatact ttccgaaccc gcttatctga cccacgcata caggtgcgat aacagaagtg
     4261 tgtctgaatt cgtcacggga gtgtatattg ctcagatgta cctcaacgac aggaatcttt
     4321 attgcggcga tggcgtctct gatagcatag ctatagtgtg tataagcgcc tgcgttaagt
     4381 ataacgccgt cataattttt tctagcaagg tgaagcttgt cgattatttc accctcatgg
     4441 tttgactgat aaacgtcgca gtgaaactca tatttcattg catgctcgat tatctcgttg
     4501 tttatgctct caaatgtatc tgtaccgtaa actccgggct cacgctcgcc cagaagatta
     4561 aggttaggac catgtataac aagtattttt ttcatataaa tactctccta tctttatttc
     4621 accgacaaat aataattctt cattgtcagt gcgtctttgt cctgagtgcc tgcactttca
     4681 cagacgaata taggtgaaag atctttcttt gcgactatct ccataagcgg ctcatagtca
     4741 ggtccaaagc cttgattatc gtcaaaagtc aggtgctttt tctcgccgcc tggatttgta
     4801 aacattatct tagaaaaatg gctgtggaat attttcagcc tgtcgtgacc gatagcattt
     4861 tcaacttcat caagcatttt ctcatattct gccttacctt ttatatagcc gaactctctt
     4921 gcgttgagat gtccgaaatc tattgtcggc aggaacgtat catcaagctt gcaaagctcc
     4981 agcacttctg ttaagttacc aagctgattt accttaccca tagtttcagg gcaaaaaacg
     5041 atatgctcaa agccctgctc aacggctgtt tttctagccc ttgtgagagt atctttagca
     5101 agttccaaag cgtcctctct gcttattttc gcacagcttc ctgaatggat aactattcta
     5161 tcagcaccca ttctgcttgc agcatcacag gattgcagaa tataatttat gctgttgtca
     5221 cgcttttcag cttcaaggct tgaaagcgaa ataaaatacg gcgcatggag cgaaagcgtg
     5281 atattatgct ctttagcctt atctttcaaa gcaaaagcta ttttatcgga gaccttaact
     5341 cccctgccac actgatattc atagtggtca agccccattg cttcgagata tcccggagct
     5401 tgaagtgttg ttttatattt agcagaaaac gcttcgctgt ttcccgcagg tccgaatttt
     5461 gccgacatta gtatcattcc tttcgtttta tgctatttga gctttggtaa aaacggcttt
     5521 tcacaggctc ttttaaatcg aaatggcaag ctcgtttcag gctttattgg gaacacgaat
     5581 attgagcgta tacctttcag atttgagcca agcacgtcag tgaatatctg atcacccaca
     5641 gccgcaacat ttttcttatt aagccccagc ttagctatag ctttggtata tccaaaggtc
     5701 agcggttttt tgccttcagg tacaaagtca agctccaact gctctgcaaa aggctttacc
     5761 cttggagcat gattgtttga aacgatcata agctttatcc ctgccgcttt catcttagca
     5821 agccaatcaa gactcgactg cgggggaacg ggattgttat gtgttgtcag cgtattatca
     5881 agatcgagga gcagcccctt gatatgcttt ttagcaagaa aatcgggggt tatctctcct
     5941 accttgtcaa aaacataagt tggtttgaac ataaaaaaac tcctgttgta taaaataaaa
     6001 agcggacata aaaatgcccg cctttaatta catattagta tttacgctta cgagcagctt
     6061 cggacttctt cttacgcttt accgaaggct tttcgtagtg ttctctttta cgaacctctg
     6121 cga
//
LOCUS       DAWEMR010000153         6098 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5104_length_6098_cov_9.214132, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000153 DAWEMR010000000
VERSION     DAWEMR010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5104_length_6098_cov_9.214132"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1660 a         1255 c         1146 g         2037 t
ORIGIN      
        1 tcgtccctta taatcttcct ctattttaaa gaggattttt cttataagct ccatatctct
       61 tttctttatt tacacctcac ctttcctgta agcaataatt gcataagggc ttttttcttt
      121 tgtttttcct gttctgactt acgctgatga agggtgataa gatggtcgag ccgagagaaa
      181 tatgctccga ttttctgttg ctcagcggat tcaggcatct gtacaggaaa gtcaacaagt
      241 atgctttgat taaggtgttt gatcgttccg ccagttgctt ttgaattaac ataatccatt
      301 aaaaatggtg ctttaagata ctgaaaaagg tacctattat ccacatcatt ttctataatt
      361 tcaacattga aaactccagc atttaatgta gccggtttat ctaaattctc gacaaatgcg
      421 acctttccga gtgttccatc cttagtaata agaatactgc cgttcttaat ttgtattttt
      481 ttgtcttgtt caaatctatc cttttcaaca tagtggcaag tagataaatt tattcttccg
      541 tcctcgaagt cagtaccagt tatcagatag taatcaccac tatccaaaaa ttctgatgtt
      601 ctaagatttt gccaaccgat tcttgcgtgc attgtacata tctccgacag cttacgctgt
      661 tcccaaggat cagtaaatcc tttaaacctg agttctggat atttactgcc ttttttcgga
      721 aacatctttt gcagataggc ttttttcagt tctttcaact gctctatctt cttttcctgc
      781 aattctatca gcttgtcctg cgtggagagt atctccgcta tcttttgctg ttcggggagt
      841 ggggggatag gcagcacaaa atcaagaaac tgagaaaaat gaacacgctg tttttctttt
      901 aaagaaccag tggaaactct attgtaatag ttaatcatag gctcagatag cagaaagttt
      961 tccatgtact ctttggagac actttctttt acagagaaaa tatcataata gcccgaaaca
     1021 ctaacatctt cttgtccctt gtatcttgct aatgcaccaa agcgaacatt cataggatta
     1081 taaacaaaat cattttcgga aacaattttg aatatatcat ccttcttgac taaatgacta
     1141 cgttcgtatc tttctgactt tggaacaacg ccttcttcta tagttaaact ataaagcgga
     1201 tacttttcaa aatcacttgt tacatttttc ttttcaataa acaactctga aaaatgcgta
     1261 tcttcccact ccacaggcac aataccgact tttgttttct tgtagccttc ggggatgatg
     1321 ccgctgttta tggcggctat gcgttttttt gtttcttcat tcatatcgta cctcacagtc
     1381 ccagttccct cagcatttca tccatcttag cctgcacctc agcaatttcc ttttctgttg
     1441 cggcaatatc agccttgaca acatcaatat caatgggggc ttcctcctcg aaagtatcga
     1501 cataacgggg aatattgagg ttgtagtcat tctcgacgat ctcactcttt tcagcaacat
     1561 agctgtattt gtccacggct tcacgcttgc ggtaagtctc gataatacgc tctatatcgc
     1621 tgtcacggag aatgttctgg ttcttgcctt tctcgaaatt gccgtcactg cttgcatcaa
     1681 tgaaaagcac atcggattta ctgcggtttt tcttaaaaac aagaatgcag gcaggtatgc
     1741 ccgtaccata gaacaggttt gcaggcagac cgataacagc gtcaagcagg ttaaactcta
     1801 ttatttcctt tctgatcttg ccctcgcttg cacctctgaa aagtacacca tgaggcagaa
     1861 caactgccat gcgtccgccg ttttcgtcaa ggctgtggag catatgcaga acaaaggcat
     1921 agtcgccctt tgaagtggga ggaactcccc atgcaaagcg tccgaagggg tcaagtgaag
     1981 cgttcatctt ctcttttttc ttgtcctcgc cactgagtcc cgagaggaaa ccgctgtccc
     2041 acttatcgag agagaatggc ggatttgcca caacgcactg gaacttcatc agcttgtctt
     2101 ccttgatatt ctgcgggttg gcgagtgtat cgccctgcca tattacagca tcgtcaacac
     2161 cgtggagaaa catattcatc ttacacagcg cccatgtctg gttattgact tcctgaccgt
     2221 aaacggctac tttgtgggac tgcactttat tgtatgcttt aaggagcaaa ccgccgcttc
     2281 cgcaggtagg atcgtaaatt ctctcatttt cctgcggcga aacaagctca gcaacgagct
     2341 ttgagacctg acttggggta aagaattcac cgcccttttt gcctgcatcg gaagcaaagt
     2401 tagctatcat gtactcataa gcatcgccga taatatcagc gctgtccaac tgcgtagggc
     2461 ggaggtcaag ctcatggaaa tcctcaagca gattgcggag aatagcgttc ttgtccttag
     2521 tctcgccgaa atcaacagtg gagttgaagt cgatagcacg gaacacgttg cggagcttct
     2581 cgctgttgtt attttcgatc tcggcaaggg caacatttat cttctgaccg atctcattat
     2641 cggtgcggtt gtcgtagaga taatcgaaag tggaagtctc agtcagtgtg aaacgctcat
     2701 tgcgcatagc acgttcaaca cgtctttcgt cgcccttgta ttcctcgata tacttagctt
     2761 tagtctcgtt gtaaacgtca ttcagatact tgacaaagag catggcgagt atgtagtcct
     2821 tgtaacgtga actgtctatc ttgtttctga agctgtcgca ggcgctccag agtacctttt
     2881 ctatatcatt tttagtagtc attattattt acctcacatt tctgtttatg cctttaaacc
     2941 aagcatcaaa tttttcacgg ttatggtcga accattgact tgttatataa tagtatttgc
     3001 cgttgaatct gaatggctct ttccagaatc ttatatgccc atttttatca tatatcttgc
     3061 ttttgttgtc agtgaaaaaa ggatatgtaa ttccgaatat ttttttggat tcctgaacat
     3121 taagcagagc aagcaattcg ttatgcgtaa agtgatattg ttttgcttca agttctcgca
     3181 tacatttctg aacgtgttta cctatctttt cctcatcatt ggacagcgtt ataggacgta
     3241 tctgttcttg ctttgaatca ccatagtgca ggggtgtgta gtctgctttc agataaacat
     3301 agaaatcacc cggattttca ttaaatgcaa taagaatttt ttgaatgaga tcagcaattg
     3361 cattggtgct aaggttagtc cagacatata cgttagtgcc atctattttc atatttttat
     3421 ctttaacgta tttggatccg aaatatgaga ttttacgccc tttgaaattc ggagaatcga
     3481 ttaatgtttc aaaagtcttt ttaggctttt ttaccagaat attgcatatt ttgattagcg
     3541 catcagtcca atcagttacg ttgtattttg taccattgta cataaagcgg catattttct
     3601 tatgagtgta gttttcgttt aaagtatgag gtttactgct gtcaaccgca tattttgcat
     3661 aatctacttt ttcctgatac atttcatctt gtgcgatgtc agaatcggac tcgaaacccg
     3721 gtataaactg agagggagtt gagtcacgat catcacttga attactttct gcgtaattca
     3781 aatacttatt tatatctctc ttgtattcct ttagcttgtc tctgtaattt ttcaagcgtt
     3841 catattcttc atcattatca ttagatgccg cagttattct gcgctctttt aaagcttcaa
     3901 ttgcttcatc gattttagat agcagccatt ctgaagcgta gcaaatcgaa tttgctatat
     3961 ccggttcatt ttcacccagg tacgcatata catcatcaat gttaatcata agataactcc
     4021 tttcacttaa ttcgattcac attacttatc aatgccttat aatatttctt tttttgctct
     4081 ataagttttt ccagcaattt tatctcctga tttgatatct catatagttc agctacttgt
     4141 ttctggcgtt taagactggg taattttatt tctgttgcgc ctaccatagt tggctttatc
     4201 tgtttcagca ttccggcgca actcatgctg aatgcttttt tgatacggtc tttgttaaga
     4261 taccacgcta tatactgcgg caatgctttt gttatatcaa ctctgacaac cacaaagtgc
     4321 gacggaatga caagattcgc atattcttct gtaatatata cagcagtata cggctccgaa
     4381 gttttgacta caatgtctcc gacttgtgtc agatagcttg ctgttaactg ttctgaagcg
     4441 tagaaaggga tagtgtcatt aatattgata gtgccgttat cgcagacagc tttgagatta
     4501 agctgacgat actcaaagcg ttctgaggca tcttttgatt ctttacgtga aagaacgaga
     4561 cctgttttta cagtcgcaat tttatttagt tccataagcc ctccaatcaa ttttgttatg
     4621 tagattatgt aacctgagct ttttaaaaat attgcccact cacaaattaa tatttgcgtc
     4681 atgagcaata tgtaactgtt tgtcttgagt atagtatatc acatatttcc ttgattgtca
     4741 atagctttta aaaaaataat tttgttatga tataaatgtg acatggtaaa agagacaatt
     4801 agtattatgt aataaagctg aaacaaagta gttctcaaaa tatgtattat aattccgata
     4861 aggttcattc cagccttatc ggaatttttg tttttggagg taattgaaat ggaagaaaac
     4921 gtaagaacct gcgacatctg cggctgtgag atcaaagagg aagagggaac atgggtagac
     4981 gatcagtttc tttgtcatga gtgtgttgac gaacactgca ccacctgcga tcactgcggt
     5041 gaaacgatct ggattaacga tgcagtacag gacgatgata ccacactatg cgaaacctgt
     5101 tacaatgact actatcaccg ctgtgagtgc tgcaacagaa ttatccatga caacaacatc
     5161 aactggcgag gcgattatcc ttattgcaac agttgttacg atgactttaa tgatgagatc
     5221 gaggagtaca gctacaagcc tgatcctgtg ttccatggca atggcaaccg ctatctcggt
     5281 gttgaacttg aagtagactg cggcggtaag gacgatgaca atgcaagcaa actcaaagaa
     5341 atagcaaata caagccatga gcatatctac atcaagtccg atggcagtat tgacgacggc
     5401 ttcgagatcg tttctcaccc aatgacgctt gactatcatc agcatgacat ggactgggag
     5461 gctctcctgc atgaagcgat aaaaatgggc taccgctctc accaaacaag tacctgcggt
     5521 ctgcatattc atgtcaacag gaacgctttt ggtgacaatc aggctgagca ggaagaagtc
     5581 atcggaaaaa tcctgttcct gatcgaaaag cattggaacg aggtcttctg cttttcccga
     5641 agaagccagt ataacatgaa tcgcaaaaca gcagatgcct aacactgatt ctgtagacgt
     5701 ctgctgttgc ttttgcttat gaagttcttt tgactgtcgc cattcttcag tgatgttatg
     5761 tttgcaaaag ttatttagat actatttgtt aaaaagtcta gaaaaaaatt gatcgaatat
     5821 tttttcaaat aagatggttg atacaaaagc tacaattgag ctcgtaaaag agactagctt
     5881 tgcgacaata ttttgatgtg aataaaattt acacattata atataaacta agaaaccaat
     5941 cgctaatgcc attacaattg caattattag tttcttttga ttctgttttc ttttaatttc
     6001 ttggtattca gattcttgtg caaatttttc ttttatcttt acaatgtcct ttttccaatc
     6061 ttcatctagc catttcataa ttccatttcc gggtctta
//
LOCUS       DAWEMR010000154         6075 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5124_length_6075_cov_11.673422, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000154 DAWEMR010000000
VERSION     DAWEMR010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5124_length_6075_cov_11.673422"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1486 a         1458 c         1300 g         1831 t
ORIGIN      
        1 tgcatggaca tagaacgtct ttcggacaag tctggatatt tacgcttcaa atcgaatttt
       61 ctcggcaaca taaggatgga gctttctaaa atgttagcga tgcttgacag ccaaaagaaa
      121 tccttctctt caaaagccgg cactgaatat gagcattttg accatgaggc aaggcttcgt
      181 atactgaaga cattttccgc aacggtgaac atgacagaac ttgcaaaata tttcagtggc
      241 aatttcacca ctgaatatca gagcctgtca gacatatgcg gtgagatatg cgacgaagaa
      301 ggcaatttgc tggaggagga cggctgtatt ttcagatact ccattgaccc gtgcatttat
      361 tttgaaacaa acaaaagcct tattaaaaat gtgcttgtaa atctgcttgt gaacgcatat
      421 atgtacaatg acaaggaaca acggcaggtg gagcttatcc taaaaactga ggacgacagg
      481 ataatactgt cagtaagtga taacggcaac ggcatgacag acgatgtatg gcaggcggct
      541 atcacgccct ttggcacatt ccagaaatac agcagcagtg aggcgttggg gcttgcactg
      601 gcaaaatgct tttgcgaccg ttttgacggt gaactaagtt atcgcagcga gatcggcaaa
      661 ggcacttgtg tgaacatgac tttcccatgt gacagccgtg gtttgccaaa agagtttcat
      721 gtgagcagaa tgccacccat accgaaccca tacggcagta cctactgcat aatggctaaa
      781 ggacttgacc cgctgaaata gggtttgggc aatatacaga gcaagtgaat aaatgggata
      841 caaaaaaggc agtgccgatt ttctcggtac tgccttgatt attttatgaa aattagtcct
      901 tgatgataga cttacctgtc atttcctttg gctgctccag acccatgagt ttgagcattg
      961 ttggtgaaag gtcagcaaga acgccgccct ctctgataag aacattgcct gcgcctgcga
     1021 taataagagg tacaaggttg gttgtgtggg ctgtgaatgg agagccgtcc tcttccatca
     1081 tcttatctgc gttgccgtgg tctgctgtga taagtgctat gccgcccttt gcaagaactg
     1141 cgtcaactgt tttgcctacg cactcgtcaa ctgcttctac ggctgctacg gctgcgtcga
     1201 acacgcctgt gtgacctacc atatcgcagt ttgcatagtt gaggataatt gcgtcatact
     1261 tgtcagactc tattctcttg agaacttcgt cgcatacaag atatgcgctc atttcaggct
     1321 ggaggtcata agttgctacc tttggtgaat tgatgagggc tctgtcctcg ttttcaaaag
     1381 gagcttctac gccaccgttg aagaagaatg taacgtgtgc atacttctct gtttcagcga
     1441 ttctaagctg tgaaagaccc ttctttgcga tatactcgcc gaaagtattt tcaaggcttg
     1501 ctggcttgaa tgcaacgtca acgtttggca ttgttgcgtc atactgtgtc atgcagacat
     1561 agtaaagtgg gaatctgccg tttcttctct caaagcctgt gaagtcgtcg tcaacgagag
     1621 ttcttgtgat ctctcttgct ctgtcaggac ggaagttgaa gaagataacg ctgtcgtttg
     1681 cggatatctt gccgttctca tcgataactg taggaacaac gaactcgtct gtaacgtcag
     1741 cggcatagga agccttgata gcgtcaactg cgttatcagc cttattgccc tcgccgtata
     1801 ccattgcgtc ataagccttg cctacacgct cccaacggtt atctctgtcc attgcatagt
     1861 aacgtcccat tactgttgct atcttgccga caccaatttc cttgagcttt ccttcaagct
     1921 cagctacgaa gtcaactccg cttgtaggag gaacatctct gccgtccatg aagcaatgaa
     1981 cgtatacctt tgtaagacct gctttcttag caagttccaa aagaccatag aggtgtgaat
     2041 tatgtgagtg aacgccgccg tttgaaagaa gtccgaacag gtggagtgcg ctgtcattct
     2101 tcttgcagtt ctcaacggct gctgtgaaag cttcatttga gaagaattcg ccgtccttga
     2161 tagactttgt aattcttgta agctcctgat aaactattct tcctgcgccg atatttgtat
     2221 ggcctacctc tgagttaccc atttgtccct caggaagtcc aacgtccatg ccgcttgcgc
     2281 cgataagagt gtgcggatag ttggcaagaa gtctgtcgat attaggtgtt ttggcagctc
     2341 tgatagcgtt gccgtaatcc ttgtgattat agccgaagcc gtccataatg ataagtgcta
     2401 tgggtttctt tgacatattt cttagtcctt tcaacatcat ttatattcgt attatcccca
     2461 ataaccgcca ttttcaagga agctcaggac atattcctta aattccttgg cagtatcgtc
     2521 gcttgtgtaa taggtctttg tgccgatacg gacatagttg ctctctcctg actcatagta
     2581 gatagctgga ccttctgaac attttatatc cagccaatcg ccaccgacaa catactcctt
     2641 tgaatccggc tctttttctt ctgcgttttc agttaccttt ttggtgaact ctgcggtttt
     2701 gtcgagaagc tcgctgtctg taaactcaac gccgctaagg agcttactag agtccatttt
     2761 gatagttaca ttttcaagct tagcctcaga tgttgtctga gagctttctg ccgctggtgc
     2821 gctgctttcg tcaacgcttc cgcaggctgc aaatgacata cacatggata atactaatgc
     2881 tgcaaatatt atttttttca cagttgcgtc ccccctgttc aaaaaaagca gaggacagcc
     2941 gcagccgtcc cctgcggaac tttttatttg tttgttcttg tgacgaatga tattttaccc
     3001 ctagatatcc gtctgttaat tagccctcaa cagcagcctg aacgatagtg ttgaagtcag
     3061 cagccttcag tgaagcacca ccgataagac caccgtctac gttctcctta gaaagaagct
     3121 ctgcgcagtt cttagcgttc attgagccac cgtactgaat tgtaactgcc tctgctgtag
     3181 cctcgtcata gatcttagca agtgttgctc tgatgaatgc acaaacttcc tcagcctgat
     3241 ctgctgtagc tgtcttgcct gtgccgatag cccatactgg ctcgtaagcg ataacggact
     3301 tcttaacgtc ctctgctgat acaccgaaga aatcaagctt tacctgtctt gcgataactt
     3361 cctctgtgat gttgcactca cgttcccaaa gaagctcgcc aacgcaaacg ataggcttga
     3421 ggcctgctgc aagagctgcc tttgttctct tgtttactgt ttcgtctgtt tcaccgaaat
     3481 actgtcttct ctctgagtga ccgataacaa cgtactcaac gccaagctca acgagcatat
     3541 ctgctgagat ctcgcctgtg aaagcaccgc tcttctcaaa gtgaacgttc tctgcaccga
     3601 tctttacgtt tgaacctgct gttgcgttga tagcagtttc aaggtttgtg aaaggaacgc
     3661 aagcgataac ttcaaccttg ccctcagcgt ttgcaacaag tggcttgata gcttcgagaa
     3721 gctccttagc ctcaggtctt gtcttgttca ttttccagtt tcctgcaata attgcttttc
     3781 ttacagcttt tttcatgata aaaaactcct ttatttttta tatggaacag aaaaactgct
     3841 cctgctagtt ttccaagtct attatacaac attcactcgt atttgtcaac taattaacaa
     3901 aggcttggac ttattttatc tgttttttta cagcttatct ataagcatga ccacaccgaa
     3961 aacgccaagc aggacaccga tagcgatagc cgcatagccg aaaggcgttt tctcctcttt
     4021 ttcctcggct tccttttcct cctcagtgac ttctaccttt gatttttcat cgtcaaggta
     4081 aaattcctgt gggttttctg ggttatactt tatgtctatc atgtcgccct tttcatagcc
     4141 gttgtctgtg actatctggg tatacttttc gccgtcaatg gtatagccta ttttccactc
     4201 ggtaaaaact ctgtcctcac cgccgttgaa gtcaggcatt gtggtcttga ctttctgcaa
     4261 ggcttttgcc tgagttggtt cgtagtgatc ctcctcaggg atattatatt cctgctcagg
     4321 gaaaaagcga tagccctgca aggctttgat accgaaatag ataagtccca cgcctgtgag
     4381 cagaagaatt attccgccta tgccctgcca gcctaatccg ctttttgcaa gtagtgttat
     4441 ttcattcatt tttactacct cgatttctgc ataaaaaagg gacaggcaaa acctgcctgc
     4501 ccctagctta tagcttagta aaatatatta cttgtcagca atcacgtcga tacctgggag
     4561 caccttgccc tcgaggtatt caagtgaagc tccgccgcct gttgagatgt gtgacatctt
     4621 atcagcaaaa ccgagctgga taactgctgc tgctgagtcg ccgccgccga tgatagttgt
     4681 agcgtctgtt tcagcaagtg cctttgcaac tgcgattgta ccctttgcaa gtgttgggtt
     4741 ctcgaacacg cccataggac cgttccaaac aactgtcttt gcagacttaa cagcctcgcc
     4801 gaagagagcc tgcgtctttg tgccgatatc aaggccttcc ttgtcagctg ggatcttatc
     4861 agaatcaaca acttctacgt cgataggagc gtcgattgga tcagggaagc ctgctgcaac
     4921 tgttgtgtcg atagggagaa gaagcttctt gccgagcttt tcagcctttg ccatcatttc
     4981 cttgcagtag tcgatctttg tatcgtcaac gagggaaaga ccaacttcct tgccctgtgc
     5041 cttaaggaat gtatatgcca taccgccacc gatgataagt gtatcgcact tctcgaggag
     5101 gtttgagata acgttgagct tatctgctac ctttgcgccg ccgaggatag caacgaatgg
     5161 tctaacaggt gtttcaactg catttccgag atagttgatc tccttctgca tgaggtaacc
     5221 aactgctgtt tccttaacaa actttgtaac gccagctgta gaagcatgag ctctgtgagc
     5281 tgaaccgaaa gcgtccatta cgaatacttc gtcgtcaacg aggtcagcaa gttccttagc
     5341 gaaagcttcg ccgttctttg tttcctctgc gcctctgaat cttgtgttct cgagaacaac
     5401 gatatcaccg tcagccattt cagcaactgc cttcttagcg ttgtcgccaa ctactgtatc
     5461 gtcagcagcg aatgttacct ttgtttcagg aagaagctct gcaagtcttg cagcagcagg
     5521 agcaagtgag aacttagcct ctgggccgtt ctttggcttg ccaagatgtg aacacagaac
     5581 taccttgccg ccgtcagcta ccagtttctt gatagttggg agagcagctg ttattctgtt
     5641 atcgtttgta ataacgccgt ccttaagagg aacgttaaag tcaactctta cgagtacctt
     5701 tttgcccttt acattaatgt catcaacagt aactttgttt aaacctgcca ttttaaagac
     5761 acctctcaat ttaatttttt aattcacgtt gttaaaaaac taacaacctt acctttatta
     5821 tataacatac cgccataaaa atcaagtatt tttttataat ttttaccctg attttataac
     5881 tttaggcttt taaagtgcgt ccacctttat ctctccgttg tcagcggata ttgccacggc
     5941 ggacactgct ccctcgccct tgtctataag cagttcggct atcttatcct ccacctcgtt
     6001 tcttatgaca cggcggatat ctcttgcacc aagcttctga ccgtatgcct tatgtgcgat
     6061 agttttcaga gcttc
//
LOCUS       DAWEMR010000155         5950 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5236_length_5950_cov_12.845123, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000155 DAWEMR010000000
VERSION     DAWEMR010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5236_length_5950_cov_12.845123"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1657 a         1232 c         1432 g         1629 t
ORIGIN      
        1 caaggtgctt ttgtgtttta taggcttttt gtgaaaaatt acgattttct ttccattgat
       61 gggagaaaat atatttataa tttttattgt ttataatttt tgcgaaatat attaagcaaa
      121 tcgttacaga aagcattcgc tttgctgtgt cttgataaat cggaggagta tgtatgatta
      181 gagaagattt aagaaatgtt gctattattg cccacgttga ccacggtaag actacccttg
      241 tagaccagat gctcaagcag agcggtactt tcagagaaaa tcaggctgta gaagaaagag
      301 tcatggactc aaacgatatc gagcgtgaga gaggaatcac tatccttgca aagaatactt
      361 ctatcaagta taaggacgtt aagataaacg ttatcgacac cccaggccat gctgatttcg
      421 gcggtgaggt agagcgtgtt cttaaaatgg taaacggtgt tatactgctt gtggacgcag
      481 ccgagggtcc aatgccacag acaagatttg tgcttcagaa agcacttgag cttggccata
      541 aaataatcat cgttatcaac aagatagaca gacctgacgc tcgtcttaat gaggtaggcg
      601 acgaagttct cgagcttctc atggatcttg acgctactga cgagcagctt gacagcccta
      661 tcctttattg ctcaggcaga gcaggcacag ctactcattc gccagatgaa gagggcaagg
      721 atatgatacc tctctttgat gagattctca gctatatccc tgcacctgag gtagacacag
      781 agggcccaat gcagtatctt gtttccgcta tcgactacaa cgagtatgta ggaagaatag
      841 ctatcggccg tatcgagcgt ggcgaaatga aagttaatca ggacgttacg atcggcgact
      901 atcatcagac taagaaggaa tatcgtggca agatagttac aatgtatcag atagagggac
      961 ttaacagagt tccatgccag agtgcaaagg ctggcgatat cgtttgtata agcggtatcg
     1021 agaacatcac tatcggtgac actatctgcg acttcggcaa ctttgaggct gtaccttttg
     1081 taaagatatc cgagcctaca gttgaaatga cattctctgt aaataattcg ccattcgcag
     1141 gcagagaggg caagttcgta acgagccgtc acctgagaga cagacttttc aaggagcttc
     1201 ttaaggacgt ttcactgaga gtttctgaaa cagacagcac agacgctttc aaggtcgcag
     1261 gacgtggcga aatgcacctt tctatcctta tcgagaatat gcgtcgtgag ggctatgagc
     1321 ttagcgtttc aacaccgcac gttctgttta aggagataga gggcaagctt tgcgaaccta
     1381 tcgaaagtct tgttattgac gttcctgaga attgcgtagg ttctgttatg gagaagatag
     1441 gtgcaagaaa gggcgagctt caggaaatgg cacctgttgg aagcagaatg agacttgaat
     1501 ttcttatccc tgcaagagga cttttcggat acaagagcga gttccttaca gacacaaagg
     1561 gcgagggtat catgagttct gtattccatg actacgagcc atacaagggc gagataccaa
     1621 agagagcaac aggctcactt gttgcgtttg aaacaggcga ggctgtaaca tacggtcttt
     1681 acaacgcaca ggagagaggc gagctgttca tcacagcagg cacacctgta tatgagggaa
     1741 tgatagttgg cgcttcacca aagcaggaag accttgttgt aaatgtctgc aagaagaagc
     1801 accttacaaa tacccgtgca agcggttctg acgacgctct cagacttgtt ccgccaagaa
     1861 atctcagcct tgaggattca cttgaattcc ttgctgacga tgaacttctt gaggtaacac
     1921 caaagagcat aagaataaga aagcgtattc tgagcaactc acagagagct aaggaaagag
     1981 ctaaaatgta ataataacaa aaagaggagc ggtcatgccg tctcctcttt acttttttat
     2041 gaaattgtag tgctattgta tgttggtgta gtagaaaata gcaagctgtg tgcggtcacg
     2101 gagggagagc ttttctagga tagtgctgat gtaatttcgc actgtgccct cactgagata
     2161 aagcagtgag gctatctcct tgttgctaag cccttttgcc acctgttcga ttatctcctg
     2221 ctctttttcg actatgccgt agtggtcaaa atcaaaactg ctgtgattgg taagaagctc
     2281 aggaagtttg cttgcaacat ctgagccgaa aactgtctgc ccattcatga cggtttcaag
     2341 ggcagggcat atgctttcaa aatcctgctt taaaatatat tccttcgctc ccataagcac
     2401 agcctttata atgtattcat catcagaaaa tgtggtaaga tacagtatcc ttgcgtcagg
     2461 gtacccagca aggatctttt cgccggcttc aataccgctc atgccgttca tgcggatatc
     2521 cataagcaga acatcaggct tgagaagaga ataaagctct atggcttcct caccgcagct
     2581 tcccatggca agaactttca cactgcctgt gctttccaaa atggttttca gcgacaccgc
     2641 cacaagcttg tcatcatcta ttataacaat gttcatgtca agacctccgt tcagtcaggt
     2701 tttgtttcgg cacagacaga aaaatgcgga atcccttgct gcccgatgtg atgtttatgt
     2761 ttccatttaa agaagccgca cgctctcgca tattggcaag ccccatgccg ctttcgtcta
     2821 tcttttcggg acagcttccg ttgtctgcga tactaagctg ataaagagca gggtgttcac
     2881 gaatgattat ttttatgctg tcgccgtcgc tgtgctttgc agtattggaa agtgcttctt
     2941 ttataacgcc aataaagcaa agcttcacat ctcgtgacat tctttcggaa aagtcataat
     3001 cacagcttac tgcaaatctg tctttcagag gtcttatgca gtcttcaaca gcaggcttta
     3061 gtgcgataga atcgtcatga agatcatgaa cgctgcttcg tatgctcgtc atggcggtgt
     3121 tcagcgtgcc gctcaggtcg ttgatggctt caccgacggt ttcatctttg tttatgaccg
     3181 agagcgcacc aagctgtaaa atagaccttg tgagcatatg tcccacgtta tcgtgtatct
     3241 cccttgcaat gcggttacgc tctttcagcg ttgcaagata tatctcgtag tcttgatttc
     3301 tctgcatggc tttgtttttc tctacaagct gggtggtaag ttcttttgaa ttgtcacgag
     3361 tatttatgag catctttgtc agcttttcgt tcagaacgct gcggcttgaa attgtgaagc
     3421 ttaccaaaaa aagagcggca agtgggaaga tgtcttttcc aagagtgaat atatttgcaa
     3481 gtacgcctat gaatgttaag accatagcag ggtaaaattt tgtacagaaa gcatcataaa
     3541 gcaccaaagg ggcgagtatc aaagcttctt tactgaaaat gcaaagtgag ctgtacgcaa
     3601 taactgaaag gtatgcaacg gcagagcttg gttttatttg aaagatagcg gagcatatga
     3661 cagccaatag taatgtggtg atgtttgacg gatctgttcc ctctgcaagc atgaatgcac
     3721 agcagctaag gcctaaaaga aacttatccc acagcttgtt catatggcgt tccccccttg
     3781 atgtgatatc tcaattttaa cacattagtg gtcgattgtc tagtatcgtt ctaaaatagt
     3841 tttgctatac tgtaaaaatc attgacttat acctataaaa aatggtataa tgataacaat
     3901 gataatttct tgtaaggagc ttatttttat gaaagacggt tttataaagg ttgccgcctg
     3961 cacccctgag atacaggtgg cagacgttga cttcaatgtg gataaaataa tcagtcagct
     4021 tgaaaagtgc agggaagagg gcgtaaaggt cgctgtgttc cctgagcttt gtataacagg
     4081 ctatacctgt caggacttgt tctttcagaa cgctttgctt gacaaggcaa tggagggtgt
     4141 tgtaaagata gcaaagacta ccgctgacag cgatatgctt gtggctgtgg gcgtgcctgt
     4201 gagagcaaac ggcaagcttt ataactgtgc ggccgtgata caagacggcg aggtgcgtgc
     4261 ctttgtacct aaaacccatc tgccgaacta taacgaattt tatgaggcaa gacattttgc
     4321 accatacaga ggcgaatgtg ctgccattga tctgagagag tacggacagc ttgaatttat
     4381 accgcctatg gaacagaccg ttttcgtatg tgaagagata cctgagcttg tggtgggctt
     4441 tgaactttgc gaggacttgt gggtggctga ccctgtttca aattatcttg ctaaggcagg
     4501 ggctacgctt atatgcaatc tgtcagcaag cgatgaagtt atcggcaagg actcataccg
     4561 cagacagctt gcttcaaacc aatctgcaag gcttgtggca gggtatatct attgttctgc
     4621 aggtgacggc gagtcaacgc aggatatggt cttttcaggt cacaatatca ttgcggaaaa
     4681 cggctccata cttgcagaga gcaggctttt tgaaaacggc attaccatat cggaaataga
     4741 ttttaaaaag cttgcgtttg agcgaagaaa aatttctacc tatcctgcaa gcgcagagtg
     4801 ggactattct tccaaggaca gcggtttttt ggataaggac aatatctccc gtgaaagatt
     4861 cagccttgaa atgggcgaaa cagctttgac aagaaaattt gcaaaaacgc cttttatccc
     4921 gtcaaatcag gcggagagaa acgagcgctg tcaccttatt ttgcaaatgc aatcccatgg
     4981 acttatgaag cgtatcgcac acacacattc caaaactgtg gtcattggca tttcaggggg
     5041 acttgattct acgcttgcac tgcttgtgtg cgtaatggca atggacttgc tgaaaagacc
     5101 gcacatggat atcgttgcgg tgactatgcc atgctttggc acaacaaagc gcacacgctc
     5161 caatgcggaa atactttgcg acgctttggg ggtaacgttc agagaggtgg atatctcaaa
     5221 agcagtttta cagcactttg aagatatcgg tcatgattat aacgatcaca gcgttgtgtt
     5281 cgagaatggt caggcgagag agcgcacaaa ggtgcttatg aatatcgcaa atcaggtgag
     5341 cggaatggtg gtcggcacag gcgacctgag cgagcttgct ctcggctggg cgacctacaa
     5401 cggcgaccat atgtccatgt atggtgtgaa cgcatctatc cctaagacct tggtaaggca
     5461 tatcgttcag tattacggcg acacctgcac atctgatact cttagagatg tactgtacga
     5521 tattttggac actcctgttt ctcctgagct tcttcctacc gacgagagtg gcactgagat
     5581 gacacagaaa acagaagacc ttgttggtcc atatgagctt catgacttct tcctttacta
     5641 tggcatacgc tggggctttg agccgtcaaa ggtgaaaagg cttgcgaaat atgcctttga
     5701 gggggattat cctgacgagg tcatagacaa gtggctcaaa acattctatc gcagattttt
     5761 ctcacagcag ttcaagcgct cctgcctgcc tgacggcgca aagatcggtt ctgttaccct
     5821 gtcaccgaga ggggattgga gaatgccgtc tgacgctgtg gctaagcttt ggctggatga
     5881 tatcgacaaa taacgcaagg taaaaataaa gtaacaaaat ctttataact gctcggtttt
     5941 acagtattgg
//
LOCUS       DAWEMR010000156         5880 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5300_length_5880_cov_9.014592, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000156 DAWEMR010000000
VERSION     DAWEMR010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5300_length_5880_cov_9.014592"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1161..1170
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5026..5035
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1724 a         1159 c         1498 g         1479 t
ORIGIN      
        1 tttcctgcac aaaactagac accccagtat gagcatctca gacctgccct tgttgctatc
       61 gagcaggata ggagcataga ggggcttgtg ataatcctca acactgtggg cggagatgtg
      121 gaagcaggac ttgccattgc agagcttatt gctagtatga aaacgcctac tgtgtcaatg
      181 gttgtgggcg gaggtcactc cattggcgtg ccgttggcgg tgtgtgcgaa aaagtcattt
      241 atagtgccaa gcgccacaat gaccatacac cctgttagaa tgaacgggct tgtgctggga
      301 gtacctcaga cgctttctta ttttgacaaa atgcaggaaa ggatagttcg ctttgtgtgc
      361 gataattcaa ccataaagcc tgagcgtttc cgtgagctta tgatgtcaac gggcgagctt
      421 gtaatggacg ttggttctgt tatcgacggg gagcaggctg taaaagaggg gcttatcgac
      481 aggctcggag ggctttccga ggcgatagac tgcctttatg atatgataga aaacagttct
      541 gaaaaggaga aagctgaaaa tgtttcagac aataatcgac ctttatgacg tattcaggac
      601 tgacgtagat atcggagaag agtttgagga tgatgatact gctgtggaca cagatccggc
      661 acattatctc agccgtgacg gcttttatct ctgacaaaca acaaaatttc ggaggacagc
      721 aatgggtatc atcaatgcga tttttcaggc tataattcag ggcttgacgg agtttcttcc
      781 agtttcaagt tcaggacatc tttcactgta tcagcatttt acaggaaaca gcggagaggg
      841 agctttgttt gtctcggcga tacttcacct tggcacgctg gtagcagtat ttgtggcttt
      901 caggaagaca atatgggata tgataaaaga gcttggcgtt atgataaagg atatttttac
      961 aggcaagttc actcttaaaa atatgaatcc tccaaggcgt atgatagtta tgataattgt
     1021 ttcctgcttc tgccttgtac cgttcctgcc atttaagggc tggtttgagg gcattgcaga
     1081 ggattcaagc attttcgcag agggtctttg tttcctttat acatcggcga tactttttat
     1141 gtcagatcgt tgtgtaaagg nnnnnnnnnn ggcgatatag cttataaaga cgcaataacg
     1201 gtcggacttt ttcagggagt tgcactgttg cctggcgttt caagatcagg ctcgactatc
     1261 tcagcgggac ttttctcagg catgaccaga gaaacatcgg taaagtattc tttcattctg
     1321 ggcatacctg ttatacttat gagctgtctg cttgaagtca aggacgcagt ttcaacacgt
     1381 gctgatatag attgggttag ctgtgcagtt ggatttgtag tagccgcatt tgtgggactt
     1441 tgtgctatca agcttgtaaa ctggctcgtt aaaacggata aattcaaggt gtttgcttat
     1501 tatacccttg tgcttggcgt catagtcctt gttatctcat ttatcgagat gtgcatgggt
     1561 caccctatat cctttgcaaa gtaatgacat aagataatac agtttctgtg aaaaagcttg
     1621 aaattctgtt ttcaggcttt tttgtgggtt ataaaggcaa ataacatttt gcctttattt
     1681 gcttaaataa gacaaatacc gaaaggaatg gtaaaataaa tggcagctca aaagaaaggc
     1741 tcaaagcctg cgccaaagca gacagcggca aagaaaaagc ccgcacaaaa gcctgcgcaa
     1801 gctgcaaaaa agaatacaaa acagcaaaag cctcagagca agccgccaca gtcaaattcc
     1861 atgacagcag tgagagaaag agagaacaga cgcttctggt cttacatact tttctttttc
     1921 ggcatacttg aactgcttat cacgttcgtg gagggggacg gcttgtggaa atggctccat
     1981 gagctgaacc gaggactttt cggagtgaca gtatttctgt ttgcacctat gatcatctat
     2041 gttgccctta tgatagcctc agatgtgacc cataacaagg tcatagcgaa ggtggtggag
     2101 ggaagcgttc ttatgcttct tataagcggc atggctcaga taatccaagt gggctctgtg
     2161 gacggaagtt cgttctggct caaacttgtg gggcttttca atgatggaaa acagctccgt
     2221 ggcggcggac ttgcaagcgc attgcttggc tggcctttgc tttcgctttt caagagagtt
     2281 ggcgcaggta tcgttatcgt gcttgtggcg ttcactttta taatgctgct tactaatgtt
     2341 acgcttccac agctcctcaa agcgatttca aagccttttg taaagagcta tgaagcggtg
     2401 aacgaggagc gtatcgagcg tgcggctcag cctccaaagc ctccaagaga gaagaaagag
     2461 ccaaggcgta atggtagggt tgatattgca aagttctatc ctgatgatgg ccctgacact
     2521 gcggcggaag cctttgtgcc tgttgcagag gcggaagaag ctaccgacaa ggtggacgct
     2581 tcaaagatag atatgccggt ccaccctgtg aaagctcctg tgataacaca tgaaaagctt
     2641 gaagaaacgg ctaagactac cgataatgag gagcttaaaa agataataga aaacgccata
     2701 ggcgacagca gagaagaaaa gaagtccaag gacgagcctg ttaagccacc agtagtgaat
     2761 gttgataaga acggtcagac aacgtttttt gagaaagata ataaaatatc tgcttacgtt
     2821 tatccgcctg tggatatact caaatatgct aaaaatcctg tggcttccga gatagtccag
     2881 caggagatac aagagaaatc tgctaagctt gtggaaacgc ttgaaacata tggcgctaaa
     2941 acaaggatcg tgggcataca cagaggtccg tccgttacaa gatatgagct tcagcctgca
     3001 gcgggcgtaa gagtgtcgaa gataacgggt cttgcagacg atatcgcact taacctcgcg
     3061 gcaatgagcg ttcgtatcga agctcctgtg ccgggcaagg cgtgcatagg catagaagtg
     3121 ccgaacgatc atagagatac ggtttctctc cgtgagctta tcgacagcga ggaatacaga
     3181 aaggcaaagg gcaagcttac atttgcagtg ggcaaggata tcgagggtaa tatcattatc
     3241 ggcgatatcg ctaaaatgcc gcatatgctc gttgcaggta caacaggttc aggtaagtct
     3301 gtttttacaa actctatcat actttctata ctttaccatg cgtcgcctga cgaagtaaag
     3361 cttattctta tcgaccctaa gaaagtcgag tttcctattt ataataagat acctcatttg
     3421 cttatccctg ttgttaccga gcctttaaaa gcggctggtg cgcttggctg ggcggtaaat
     3481 gaaatgaaca agcgttatct tatgttcgag gcgaacaatg taaagaactt gcaggagttt
     3541 aacgacatgg ttcttgaaga acgcaataag cctgccgagg agcaggacga agtccgtgca
     3601 aagatcgacc tgctgccgca gatagtcatt gtggtcgatg agttcgccga tcttatgatg
     3661 gctgcgagaa gcgaggtaga ggactcggtt ttaagacttg cacagctcgc ccgtgcggca
     3721 ggaatacata tgataatcgc aacacagtcc cctagggcgg acgtgttgac aggacttatt
     3781 aagtcgaata tcccatcgag agtttctctc agtgtatcca gcaacgttga ctcacgagtt
     3841 atacttgatg aaagcggtgc agagaagctt cttggaaacg gtgacctgct ttataagcct
     3901 gttggcgtta aaaagcctat aagaatgcag agcggttatg cagcgacttc tgagataaga
     3961 gaggttgtaa acttcctcaa gaacgagcat accgcagaat acagcgacga agttatcgca
     4021 gaggtggagg agaatactcc acagccaaag gacagcggtt cggcaggcag cgataatgtt
     4081 tccgtaaatc ctgatgatga ccttgtaaat caggcaatat ccataattgt gcagacaaat
     4141 aacgcttcca ccgcgttctt gcagagaaag ctaaagctcg gtttccctag agccgcccgt
     4201 atcatggacg agatagagga aatgggcata ataggaccac aggaaggctc taaggcacga
     4261 aagataaaca taacaaaaga agaatgggct gaaatgcagg caagaagata gctcaggcgg
     4321 aaagggataa catggcaggc agatctgcga aaaagaaaaa ttcgacagca ccgcttaaaa
     4381 aggcggtaac gctcatagcg gtttttgtgg tgctgctttt tacagtgctt gacctgacgg
     4441 ggttagtttc tttcgacagc ttgcttatta aggcaggcgt tgaggacaag ccggcggaac
     4501 acgctcagac agaggtacat tttatcgacg ttggtcaggg cgacagcacc cttgtgatat
     4561 cacaagggga ggctatgctc attgacagcg gcgataaaga tgacagcaat aaaatagaaa
     4621 aatatcttga aaagcaggga gtgacagagt taaaatatct cattgccacc caccctcacg
     4681 ctgaccatat tggtgaaatg agcgagataa tagaccagtt tcaggtggat aaattcatta
     4741 tgccgaaagt caaggctgat atgacgccca ccacaacgat atatgagaaa atgctcaaaa
     4801 gcataaaggc aaagggcttg aagataacac aggcaaagcc tatggaattt gagcttggaa
     4861 gctgtaaggt gaagcttttc acacccaaaa aggattatga tgatcttaat aactattcca
     4921 cacttgtgaa gataacagac ggtgagaaca gctttcttat cacgggggat tgcgagacca
     4981 ctgaagagaa agatatactt tctcagggtt atgatgtttc cgcaannnnn nnnnnttcgg
     5041 gggtcgattt tcttaacaag gtcatgccga gatatgctgt tatatcctgc ggtaagggca
     5101 acaagtatgg tcacccacac gaggaaactg tcacaaggct gaaaaaatat gcttcccatt
     5161 tgtatgtcac tgcagatgtg ggaacgatag ttttcagctc agatggcgag gggttgagcg
     5221 tttctgctga aaagggagaa aagtgatatg gaagagattt ttatagttga caggatagaa
     5281 aacggctatg ccgtgtgcga aactgagcag ggagaaaaaa aggatatacc gctttctgga
     5341 acaaaagacg ttcacgaggg tgacgtgctt atcttaaagg acggcgttta tatcccagat
     5401 aaggacaaaa cagaagcaag aagaaaaagg atactcgccc tacaggagga tctgtgggct
     5461 taaatatatg ctgaaaggta tagatagaaa tggaaaaaga tttatttacg cttatcaatg
     5521 aaaagctttc aggcttatca aagggacaca agcttattgc gaactacatt ctctcccact
     5581 atgacaaagc ggcttttatg acggctcaga agcttggaaa aaccgtgggc gtcagcgaat
     5641 caacggtggt aagatttgcc tcggagcttg gctatgacgg ctatcctgcc cttcaaaaag
     5701 gcttgcagga gcttatgaga aacaagctta caactgttca gagaatagag gtcacttccg
     5761 accttatgag cagtgacaac gtgcttgaaa gggtactcaa cctcgacatc gacaagataa
     5821 ggcgtacttt ggaggaaacc tcaaaggagg actttaacaa tgccgttgac actatcgtat
//
LOCUS       DAWEMR010000157         5876 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5307_length_5876_cov_8.635973, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000157 DAWEMR010000000
VERSION     DAWEMR010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5307_length_5876_cov_8.635973"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1781..1790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3457..3466
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1786 a         1361 c         1173 g         1536 t
ORIGIN      
        1 ttatccatat tctgcaaaga ctgatctaca gccgaattgc tcagcttgtc ctgtatcctt
       61 tttataagat atccgcccgc tttaagaaac gatatcatgc ccccaaggct aagtataaag
      121 gaaacgatat attttttgaa aaacgcaaac aatattcctg caaacatgac aacaacgcct
      181 gccgccattg cccacagcac ccactttggc ggaacgggtt ctatatactt gcccatgatg
      241 cccacgatag ttacaccgcc gtagaaaaat cccgaatacg caaacggcac tatcataaga
      301 agcttcaaaa taagataata aacaggctca ccgttcggtt ttgtgcgttt cgcctttttc
      361 atcatctttt ttgccgagcg gctctgcctt tctctggctt cctcacgctc tctctgttct
      421 tcaagctcct cttcttttct gagcctgtcc tgcttttctt tctgtgcttt aagcctgtct
      481 ttttttgaca taatgtacct cttttatgta ttaaaatatt atcgtatcat agttttctca
      541 tcagatattt tattatatca tagtatgcca cgaaattcaa cacatacaaa tgaaattttc
      601 ttcctttgca ctgttaagaa ggcagaataa aattgaaaaa ggctttccaa gacaaataat
      661 acttatgccg acggctgaat taaatttcta gacaggtcaa caataaaagc gaaaagaaaa
      721 acagaaagga acgtgaaaaa aaatgtcgtc atttttcaaa aaaacagcct gcgttacagg
      781 gataatactg ctggggctgt ttggtcttat aggattttat tccacaacgc tccccgattt
      841 ctaccttgtt tcaaagggca gcgagcttag cgtgaactcc tttttcacca tatcctcaaa
      901 gccctgcgaa agcaaggtga cagtggcggt gtcaggcggt tcgtcaggag cgtcacgata
      961 cacaaaaaat atgctcatgc tttttggggc agtgccagtc aaggaggttg agtcaaagac
     1021 aatggaaaga ccaatgcttt atccctgcgg acagccattc ggcataaagc ttctcacaga
     1081 gggcgtaatg gtagtcgatc tgcaaaaggt ggattcttca agccctgcca aagattgcgg
     1141 tatcagagag ggcgatgtta tagtatctat cgacggagaa aaagtcaaaa gcaacgcaga
     1201 cgttgcaaaa ataataagaa gctcaaacgg cgaagcctgc tcagtgcgca taaagcgtgg
     1261 aagcaacgac ctgaccttca agctttgccc acgccttgag agcggaagct ataaagcggg
     1321 aatgtgggtg cgtgactcct ccgcaggcat aggcactctc acattctatg accctgaaaa
     1381 cggaactttc ggaggtcttg gtcaccccgt atgcgacgca gacacaaaag aacccctccc
     1441 cctctccgca ggcacagttg gcgaaataaa cctcacaggc ttcaacaagt cacgctcagg
     1501 ctgtccggga cagcttttag gcgagtttgc aaactccgcc tccacaggtg atatcctgaa
     1561 aaactgcgaa agcggcgtgt tcggcacact gaatgcaaat ccttgcccaa atgcaaaaga
     1621 gtttcctctc ggcttccgtc aggagataca ccccggcaaa gccaaaatac tctcctctat
     1681 tgacaatgat gggccaaaag aatatgagat atctatcgag cagataaatc tctcagattc
     1741 tgccgagcat gaccttgtta tcaaaataac tgacaagacg nnnnnnnnnn ggctcgccta
     1801 tcattcagga cggaagactc gtgggcgcag tgacccatgt ttttattgag gacaccgcca
     1861 aaggctatgg aatcttcgct gacgaaatgt attcaaagac ctcagagctt gccgaaagca
     1921 ccatagaaaa cgcaggttag cgacctttgc cgatcgctgt cgatttatga aaaggctttg
     1981 catagagaaa aatatcatct gcagtcgaac aatgcttaat tatcttgttt tggcatattc
     2041 acgcactgat atttgtacac tttgcattaa taagtcgata atttgttgac gatttttcta
     2101 aaaaactatt gcatttaaca aataaatgtg ttatcattta tcttgtgaaa gggagttaaa
     2161 acaaacgctc tcaaacaaaa atgacagatt atacgacgga ggaaatgaca atgacaaaca
     2221 agatcaaaat tcttattggc gacgattcgg cacagtacgg ggtagcatgt gcttcctcac
     2281 tgagagcaat gggctttttc gtgataacac gtcccaagga cggacttact atctatgacg
     2341 ctatcaaaaa cgaacagccg gacgttgtta tcgcagacgc agtgatgccg agccttgacg
     2401 caatcgagct tatcagaaag atacagaact cgtcctatca gaaaccgctt tttataatca
     2461 caagcgccta tgagaactca tttatagaaa cacaggtcat gaacgcggga gcatcatatt
     2521 tcatgctcaa gccattcgac ataaagatac ttggcgagag gataaagtcc atgctggata
     2581 tcgacaccga tatatcctcc gactcaagct atacgaaaaa caaacagagc ataaaccttg
     2641 agataatcgt cacagacata atccatcaga taggcgtccc tgcccacatc aagggctatc
     2701 actatctcag agaagctatc atacagtccg tgaacgataa ggaaatgctt gaaagcgtta
     2761 caaagctatt atatcctgca gtggcaaaaa aattcgcaac tacgccctcc agagtggaaa
     2821 gagccatacg ccatgccatt gagatagcat gggacagagg agatatcgac accctcaaca
     2881 gcttcttcgg ctacacaata aacacaggca agggcaagcc cacaaacagc gaatttatcg
     2941 cacttatcgc agacaaaata agccttaaat tcaagaccac catggcggtc taggggtcat
     3001 aagtcatata tgcaaaaaga gcagaacgca ttttcacgtt ctgctctttt gttacttgtt
     3061 gataaatgta ggggcgaaca gtgttcgtcc gtctttgttc gcctgctaaa tttgcaataa
     3121 gacagacttt ttcgctttgg gtgtgacgtt gcatttccga acacaggcga cctcaggtcg
     3181 cccctacaca caaaatacaa aaaaagaggg cgaaaatgat ccgccctctg cattttcata
     3241 ttatcacagc tttatctctt cgctgttctt ttcgtccttg ctgttgtcat gctctatcct
     3301 gtcacggtga gtttcaagag gagctgaatc ctttacgccc tcagtggctt ctggtataaa
     3361 gcaggtcttt accgtcataa agaatattct aagatcaagc ataagcgact gatgctctat
     3421 gtacataagg tcaagcttca gcttgtcata aggcgtnnnn nnnnnngtag tgttatactt
     3481 tcccattacc tgcgcatagc ccgtaagtcc agccttgact ttcagcctgt atttgaactc
     3541 aggcattgtt ttttcatact tctgagcgat ctcaggccgc tcaggtctag gccctacaaa
     3601 cgacatatcg ccctttagta tgtttataag ctgcggaagc tcgtctatcc ttacttttct
     3661 tataaatttt cctataggag tgatacggct gtcatttttc tttgcaaggg tcgcgccgct
     3721 tttcttttca gcgtcaacta tcatgcttct gaacttgtat accttgaaca ccctgccccc
     3781 tgttgtgagc cttttctgag aataaagcac aggaccgcca tcataagcct ttattgcaat
     3841 agccgtcacc agcatgaacg gtgacgccac caccagtgcg gcagccgaca gcataatgtc
     3901 aagtatcctc tttaccgcac gctgttccca gcgcaagccg tcatttctcg ccagcaaaag
     3961 gggcgtatca aaaaggtgga aatcctccgc accccttacg ataatatctg acagcttagg
     4021 gtttatataa gtcctgatag aattttcaaa agtaaatttc aaaagcttgt ttctcaggtc
     4081 atttggaata tccgagatta taacagcctc gtgttttaat atctcacgct cgatatcctc
     4141 aaggctctcc tcacagctta tagaagcgca tatcctgtat ttgtcatttc tcacgctcat
     4201 ttttgaaaca aggtacttag cgttcttgtt tccataaacg attatcattc gccgtggagg
     4261 gtataccgcc tgatatatcc aagtgaaaac atagctccac gcaaaggcta tggccacctg
     4321 aattaccgtc ataacaagta tgggcgctat gttgagcctg cctcgtccaa taagagaaac
     4381 gagtataaat tctataaaat tcgcacccag cacacccagc acctgcgagc cgaaaagccc
     4441 cgtgaattta tcatatccaa gacggaagcc gttaaaggtc tttgccatgg atatatagag
     4501 caccgcatag atagccacca taagaatgtt accattatgc cagaacgggt cacgcatcgc
     4561 cttgttgtaa aagttgttcc aaacggtgtc gaagcacttc ataaaggcac agattataac
     4621 aagtgcgctg agatagttaa gtatcttttt gaactgatct ctgttattca acaaaaaacc
     4681 gcctttctct gctgtttttg ttatctgctc aatgagtaaa gtatagcata cttttcgcca
     4741 ataatcaagc attacacaca attttcaccg actgccacct ttttcagcct gatttttcaa
     4801 gataatacct gtatctccat tcgatatagt tttaaactat gacagaccat agaataaaaa
     4861 ttattgattt actaggaatt ttgtgttata atgtactttg cagaaaaata aaaaaatata
     4921 tttttatata gataacagaa aagaggaaaa taaatgaaga cctgcgagct tttcaagaaa
     4981 aagacaacat tatcctttga ggtgttccca ccaaagagtt caacgcctgt tgagagcata
     5041 tacagcacca tatcacagct ccagccactt aatcctgatt ttatcagcgt aacatacgga
     5101 gcaggcggaa gcacgaacaa tgcgactatc gacatatgct cggctataaa aaatcaccac
     5161 aagatagagt cggtggcaca tcttccatgc ctttatcaga caaaggaacg agtattagaa
     5221 cagcttgaag cgatgaaatc tgcgaatatt gagaacatac ttgctttgag aggtgacaga
     5281 aaccctgatt ttgagccggc aggtgatttt cactacgctt ccgaccttgt atctttcata
     5341 aaagaacaca gcgacatgaa cgtaataggc gcatgctatc ctgaatgtca cccggagtgc
     5401 gacagcatag tagatgatat aaagcacctg aaggataaag tagacgcagg ctgttcacag
     5461 cttatcaccc agcttttctt tgacaactgc acattttacg atttcagaga aaaaacagcc
     5521 atagcaggga taaacgtgcc tatcgaagca ggaataatgc ctgtgaccaa caaaaagcag
     5581 atacttagaa tgacaactct ttgtcatgcg accctgccga agaagtttct aaagataatg
     5641 gacaaatacg agaacgaccc aatagcaatg agagatgcag gcatagccta tgcggtatcg
     5701 cagatagtag atctcatcgc caatgatgta gacggcatac acctttacac catgaacaat
     5761 ccatatatag cagagaaaat atgtgaagcg gtgaagtcgc ttttctgatt tacacaaacg
     5821 caagggcgct gtaaaaaaag cgcaacaaag cccgtggatt ccctcgatat ggaagc
//
LOCUS       DAWEMR010000158         5875 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5310_length_5875_cov_8.511340, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000158 DAWEMR010000000
VERSION     DAWEMR010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5310_length_5875_cov_8.511340"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3302..3311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2115 a          978 c         1287 g         1485 t
ORIGIN      
        1 gtgccaaata atttttgcaa ttaataatat taagagacaa tttatatgat ttaggagcat
       61 aacaaataga gcttttaaag ctgttagtta tgctcctttg tattttataa gtaaaactcc
      121 gtttattgct ttaaaagcag aaacggagtt ttttatgccc atttttaaaa actggcaatg
      181 ctatattgcc aaatatccgt aagccttcgt tttgattcaa aacaaaaaaa tcaaaacgga
      241 ggaataaaga atgagtaaaa atacggttcg tgtttatgac acgataacca agaaatacgt
      301 tgaagtcgag gtgaatgaaa cggttcgtac atattacgac agaacgcagt ggaatattga
      361 agataatgac aagtcgttct ataagcacga gatccagttc tctgcgttga aaggaaacat
      421 taacggagaa cttgaaaatt tcaaggagtt tcgtactgat gatgattcgg ttgaaaagtg
      481 tattatcaga caagcggaat attcggagct gtataaatgt cttgctagcc tacctgatga
      541 tgagcactca ataattatca tgatatattt ttgtggcaaa accgagcagg aagttgctga
      601 tatattacat acatcacagc aaaatgtgca caaaaagaaa cataggattt tgtgcagact
      661 aaacaaattt ttaaaatctc aataaaaaac ggttgttaaa ctccttgttt cttcccctat
      721 acaagtgtaa ggggaaagaa aagctcctta agcatcttga aaattgaata tcaactctgc
      781 aagcacgtta atcacacggc tggtatgaaa tctcggcagc cgcatgaacc atacgccaag
      841 acctcaaacg agcgagcgat aactatgacg ttcagacctt tgaatagctt tcaaagtcgg
      901 cgatgaaacg tgtgaggata atgatacttc caagtgaggg ctgatagagc gggtgagacg
      961 cccatgagat cgatacgctg tcgatcgcct gcagagttcc gccgcagcgg atatcgagga
     1021 taaatactgc gggaaacgtc cgaaacaaaa tggatcggac gaaggcaaaa cgaattaaag
     1081 ttataccgca ctgttgtttt gcagtgcgga tataactcaa gtgcagaagc gttttttctg
     1141 catttgagtt gtatatagat tggaggaaga tgagttgctt aataaaaaag acattgccga
     1201 ttgggataaa atccgcaaga ggtttcttaa aataaagaac agaaattatg tttatgtcaa
     1261 cgggcaagaa tgccgaaaat gcgtatggtc aaatacggaa tcgggaaaaa tattttgtac
     1321 aaaaatgtgc aggaagaaag gcgataagat atgactaagt tcggacaggc agagtataac
     1381 gactttatgg caaggatatt cgcagataag cttgataaac ttcttaaaga aagcgaaaaa
     1441 cagaattaca tcaacagaaa tacagaagat atatataaag aatttgcgtc atcaaaagac
     1501 gcagcgtagg aggtatcgag atgttaggat ttgttattgg aatatttatc ggcgaaatta
     1561 ttggagtatt cacaatgtgt ctgtgcagag caacctcaga agcagacaaa tgtatggatt
     1621 ccacagattc tgaggaataa atttgttggg tatggtgata ccattcagta aaaatttgtg
     1681 gtatccttgt tagtaaaaca gaggtgaata atatgcctac agtaacggtg atacagccga
     1741 tcatagcagc agaacaaaac acaaagatcc gttgtgccgc atattgccga gtatcaagcg
     1801 attcagaaga tcagctcaat tcctttatgg ctcagacaag atactacagc caagttttta
     1861 aggattcgga aaccgaagaa ttgattgaca tatatgccga cgaaggtatc acaggaaccc
     1921 gagaggacaa gcgtgacgag tttcagcgta tgctaaagga ttgccggcgt ggtaagatag
     1981 acaggattta tacgaaatca ataagccgat ttgcaagaaa caccaaagac tgcctaaaaa
     2041 gcgtccgaga acttaaagct cttgggatta caatattttt tgagaaagaa aatatcgata
     2101 ctgccaatat gaccgacgaa atgatgatca ccatcatggg cggtctggcg caggaggagt
     2161 ccacttcgat ttcacagaat atgcggtgga gcataaaaaa gcgaatggaa aacggtacat
     2221 ttgagctgtc atgccctcca tacggatata tcaaagaaaa tggaaagctt gtggtcgatc
     2281 ctgctcaggc acaaactgtt cagaatatat tttcatggta tttaagtggt tatggaataa
     2341 tatcaatagt aaacagacta aatcacttta atactccttg ctgtaaaagg agcaacaaat
     2401 ggcatatgtt tggggtaaca tacatactta caaatgaaaa atataccgga aatacaatgt
     2461 ttcaaaaaac atattcaggc actaccctgc ccattcagcg gtataagaat aatggtgagc
     2521 gtaacaaata ctatgcagtc aatactcatc ctgcgatagt atcacaagat gattttgaca
     2581 aagtacaagc tcttatgtca gaaaaaggcg agatattcta caaaactgaa aaacagaaat
     2641 gtgttctgag aaaaataatg aagtgtgggc aatgcggcag tacttatata cgaaagaaaa
     2701 gccgtgataa gtattactgg tcatgccgaa aacatgattt gaaagccagt gaatgttttt
     2761 ccaaacgcat acccgaaagc gatatttatt atgcgtttat cagaatatgc aataagctga
     2821 taagtaatta tactcaaatt ctcatacctc ttcaaacagc attacaatac ttaaagctga
     2881 aaaaattcag cggtcaaact caggttatgg atatacacaa agaagtagcc aagctcaaag
     2941 aacaaactca cgtccttgca agacttaaaa caaaaggctt tcttgacgag gctaaataca
     3001 ttgagcagac tactgagctt acggcaaaga tcaacaaact gcaagcagaa ctgaaaaagc
     3061 ttacacgctc agacgatgag gacgaaacac ttgatcagat agatatgctg atcgacttct
     3121 ttgagaaaag agaaaatcct atgacagagt ttgaagaaac ggcatttgag agtatagttg
     3181 agaagattgt ggttatagat caacatgagt tggagtttca tttgatcggc ggaataaagt
     3241 ttaaagaaaa tatataaaac gcattgacaa tttagcataa ttatgataaa atatatttag
     3301 gnnnnnnnnn natgagcatg gacatgaata tgataagacc cgtttcggat ctaagaaaca
     3361 attttgcgga tatttcaaaa accgttcatg aaacagcaca acccgttttc cttaccaaaa
     3421 acggttacgg tgatatggtt gtcatgagta tggaggcttt tgagaaaatg cagtttgaaa
     3481 gcgacgtgta tttaaagctt gcagaagccg aaagagaagc tgaggaaaca ggcgaacgtt
     3541 tttcttcaaa ggatgttctc agcgcaatga aagcggcggc gagaggagaa taatgtatac
     3601 gattgaatat ttaccgattg ccagacgcga tatggtcgat attgcaaaat atatcggcgt
     3661 aaagcttgca aatccggatg ccgctgaaag acttgcggag aaaatggttg aatctgccga
     3721 agatttggca aatatgccgt ataagtgtcc gatgtatatt ccggtcaagc ctttgaagca
     3781 tgagtatcgt aagcttatag ttcataatta tattatgttt tactggatag acgaagataa
     3841 aaagcttgta acgatagcga gagttgtgta ttccggacga gattatgaaa agttgttata
     3901 actgaatcta aatgctttgc aggtaaaatt gcaaggcatt tttttgttgc tattggtaat
     3961 agcttttgag aagaaaatgt gatattattg tagtctgaaa ggagtgaaaa taatgccaaa
     4021 gaatcgtaca atcccgttcg gatactgtat gaaaaatggt gagataacaa ccgagccaaa
     4081 agaagtatac gccgttgttg caatatttga cgagtatctg aaaggcagaa gtttgtctga
     4141 gattgcaaag cttatgcagt cagagaaaat tcgttacaat gctatttcgg ataagtggaa
     4201 caaaaacatg gtcaagcgaa taatcgaaaa tgagagatat cttggaactg agaaatatcc
     4261 gcaattgatc gccgaggaca ttttcagaca agcaaatgaa aagcgggtta aaaaagcaac
     4321 cacgcttaat ctgatatccg atgatttaca ggaaattaga aatgttacat actgcgccga
     4381 atgcggtcac aaactttcaa ggataggcgg caattcaaag tatgagcatt gggattgcaa
     4441 aaatcacgat tgttacaagc ttgaatatcg gattacagat caaatgatca taggtgctgt
     4501 acttaatgtt attaattcgg caatagcaaa tccaagtctg ttggaatcgg gcggcgagat
     4561 aagcacatat tcacctactg cggatgtgat acgaaaacag aatgagatca atcagatgac
     4621 agattctccg caagtggatt ttgacagagt aaaatccgaa attttcaaac ttgccgagat
     4681 gaaatatgat tgctgcactt acaatgacag cccgcagaaa acagaggaaa tcaaggcttt
     4741 gcttgagaat cacaaacaat taaatacact cgatattggc ttatttaaag cgtgtgtttc
     4801 acgaatttgg ataagccatt tttgtgtcat agaggttgag cttattaacg gaatacgtat
     4861 taagaatatt acggaaagga atgagaaaaa tgtccacagc gccgaatgta acgataattc
     4921 ctgcgaaagt gcaaacagcg gaaagcaggg ataaatacca ccagttaaga gttgccgcat
     4981 actgccgagt atctacggct caggaagaac agcagaacag ctatcaggtc cagatcgcat
     5041 attacaccga tcttatcaac agaaaaaagg aatggacgct tgcggggata tttgcagatg
     5101 agggtataag cggaactcag acgaagaaaa gaaccgagtt taaccgcatg ataagaatgt
     5161 gcaggaacaa aaagatagat cttgtgatca ccaaatcaat atcaagattt gcacgaaata
     5221 ccgtggattg tcttgaatat gtcagacaac ttaaagactt gggtatcgga gttatttttg
     5281 aaaaagagaa cataaacacc ttaactatga catcggaatt tatgatagct ctgtatggaa
     5341 gttttgctca ggcagaatca gaatccataa gtaaaaacgt aagctggggc aaaggaaaag
     5401 cctaccgtga gggcaaggtg cagttccagt ataaatatct gcttgggtat aaaaaaggag
     5461 cagacggaaa accggagata gtacccgaag aggctgaaac agtaaggctg atatatacgc
     5521 tgtttctcga cggatacagc atgagcagga taaaaaagct cctcgagaac aaaggtattt
     5581 tgacttcaca gggaaataaa gtatggaacg aatcacttat ccgcagcatt ttgaaaaacg
     5641 aaaagtatgc aggcgacgca cttctgcaaa agacttttac ctcagattgt atcactcaca
     5701 agatagttaa aaatcacggc gaacgcccga tgtatcttgt gacagatcat catgaaccca
     5761 taatcgaccg tgacacatac aatagagttc agcaggagct tgcaaggcga agttctaagc
     5821 gtaaaatcag cgataaaact actaccgagc aaggaaaata cagcagtaaa tatgc
//
LOCUS       DAWEMR010000159         5873 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5314_length_5873_cov_24.109316, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000159 DAWEMR010000000
VERSION     DAWEMR010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5314_length_5873_cov_24.109316"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1462 a         1409 c          986 g         2016 t
ORIGIN      
        1 caacagttca aaattcaccg cagccgaaaa aacatcaatt tcaagctttt cggacttatc
       61 tgcaaagact tcaactgtaa acccgttaca gcgaacatca ttaccgtcct tatcctcccg
      121 atgcagatca gtatcgggag atattttcac ataaaaacca ttgtatttca ttcaaccact
      181 ctccttaaaa tctcatctgc atatcatcgt caaggtcgta atcatcatca aaatcctcat
      241 cgaattcatc attcacgtaa tactcaagct gctgagaatt ttcaagagct gtttgaagat
      301 atttctcgtc caaaccgttt tctctgtatc cctgcaaaat cgtgttgtaa tactgctgac
      361 tcggtggaga aattgtccca cgattcatca gatatgccat accttcgtag gtttttccgt
      421 ccatttcaaa ggtcattttt tcctgcctgt agaaactggg gtagccttcg tatttgttaa
      481 gagtaggcaa atccctttcg tcaagctccc atatcagaac gggaactctt gcacccttat
      541 tcggaacgat tgttgcaacg cccctgaact ctaattcata atccttgatt tctgatgttc
      601 ctacaacctt tgaatgtgga catctgtatg ccatttgctc caaatttata ttgcttccat
      661 atgcaatata taactgttta tttttcattc tctacctccg atcacattga catttccatg
      721 cccatttctt catcttcttc aaagttctca cattcgctct ctgtcggctc tgaattttca
      781 tcggatacat tttcgtttaa ctcctgaacc tcgcttacag gctcgacacg ctgcccacgg
      841 gcggcttgcc gttcagcttc acgtttttct ttcagctttg ctcttgcggc tattccgtcc
      901 tccgggtgcc gccatgcaat atcgccatcc aaatgtttta gcaaatgcgt tctgcaattc
      961 ttgaactcat ctccgataag tccaagattc aaaagccata cacgaaagct gtaccgcata
     1021 ttatcgcttt gcgaaacgtg cggaaaacag tatttcttag tcattgccgc attgcttatg
     1081 gcaagcgcaa gaactatctg acttcttacc tctcctgcat gaagacttcc gttgtatgcc
     1141 cttatctcgt aatgaccatg ctgaaagaat gagtgcagat tgcagataac atatcggcta
     1201 tttgagtaat gctgaaactg ttcgctcatt ctgccgtgat accaaagtct ctttatttct
     1261 tccatatctt taggcttttt tcggttaatt tcctcgatga actgcttatc catcttgtga
     1321 cagtaggatt cacgagcaga agaaacctgc aaagcgtccc acagaaagtc ctccttactt
     1381 gcaaaaatat ttacaagatt gcgtatctgc tgcggagtgt aatcgtctgc cgaaatgtgt
     1441 atatgcgtgc ctgcacaata tctcggaccc gttactccgc ccgcttttct aagagctcgt
     1501 acaacctcct gcaaaagcgg aatatcccca tattcaagaa caggagaatt catttccaca
     1561 ctgtaggatt tggaagctcg gtcgccgttt gcattgtaac agttgatact tccgtcgtac
     1621 acaatagacc aatccctgct tttaatatct ttaaccgtat atttatcata agtgccaccc
     1681 tcatggacaa cctctcctcc cagaacacct gaaagcgctt ttgccgcctg acatcttgta
     1741 agacccgtca tttctatctc aatgccaaaa tttcttgttt ttatcctatc aaaattgact
     1801 gccaaataac cacctcccaa caaaaacagc ttgcagatct ctccgcaagc cgtaaattta
     1861 atcgttattc aatttcagcc ctgacctcgt atccgccctt gaaaatcacc agtatttcgg
     1921 ttttagatag tacttttatg cactcgatta atttgcgtat cagcacatta tcgaaggttt
     1981 ccaactcaaa cttttcattt tcaatcatat ccataatttc atcaagtttc gcctgtgttt
     2041 cttctgaagt tttgttctgc aatttcagat tttcaagacg ttcgctcaac tgctgttctt
     2101 ccgcataaag cttcgcaaac tcgctgtcaa gcttatcctc atcgcagccg ccggaggcta
     2161 tcaaattgac taaatcattt cttgcttggt cgatttccct aagtcttttt cctgcagcta
     2221 aaatttcctc ctgaccttga cattcaagca ccgaaccaat attcgctttt aagatccttg
     2281 caatatcatt tcggcaggaa tagtaattgt ttatcgcccg aattatcgcc ttatggagct
     2341 gttcttcctt tatcgtcggt gaatcggggc agtacttttt accgtgttca agacggctta
     2401 tgcaacgcca gaccacctgc tttttgcctc ttgccgccca agtggttctg cgatagggtg
     2461 taccgcattt ttcgcagata agtaattcgg taagcgcata tttactcgaa tatctgcctt
     2521 gctcagtagt agttttatcg ctgatcttac gctttgaact tcgccttgca agttcctgct
     2581 gtactcggtt gtaggtgtca cggtcaacta tggggtcgtg gtggtcggtc accagataca
     2641 tcggtttttc gccgtgattt tttacaactt tatgagtaat acaatcaaga gtaaatgact
     2701 tttggagcag tgcgtctcca acatatttct cattcttcaa aatactctgt atcagtgatt
     2761 cattccaaat tttcttaccg gttggggtca aaagcccctt actttcaagg atttttttga
     2821 tgtttctcat gctatatccg tccagaaaaa gcttgtagat cagccttact atttctgctt
     2881 cttcgggaac gatctcaggc ttgccgtctg cacctttttt gtagccgaga agatatttgt
     2941 attgaaaact aaccttgccc tcacgaaagg ctttttcttt gccccaactt acatttttac
     3001 ttatggattc actctcagcc tgtgcaaaac ttccgtaaag tgcaatcata aattctgagg
     3061 tcatagtcat cgtattgatg ttctcttttt caaaaacaac tccgatgcca aggtctttca
     3121 attgtctgac atattccaag caatccacag tatttcgggc aaatcggctt atggattttg
     3181 tgatcactaa atcaattttc ttgtttttgc acatcctaat catgcggttg aactcggtac
     3241 gttttttcgt ttgagtgcct gaaattcctt cgtctgcaaa tattcctgcc agcgtccatt
     3301 ctttcttttt gttaataagg tcggtgtagt aagcgatctg cacctgatag ctgttctgct
     3361 gctcctcttg ctctgtactt acacggcagt atgcagccac tctcaattgg tgatatttat
     3421 cacggtttat ttccgtctgc gctttcgcag gaattattgt tacattcgat gctgttgaca
     3481 caattctcat tcctttccgt tatattttta attcgcactc catttatgag ttcaacctct
     3541 atggtgcaaa aatggcttat ccaaattcgt gaaacacacg ctttgaataa gccaatatcg
     3601 agcgtattta attgttcgtg attttcaagc agagccttga tttccgctgt tttctgcgga
     3661 ctgtcattgt aagtacagca atcgtatttc agctcggcaa gcctgaaaat ctcagctttc
     3721 actctgtcaa aatctatttg agcagaatct gtcatccggt ttatctcatt ctgctgacgg
     3781 ataacatcga cagtagggga atacacgctt atttcgccgc cgtactctaa taggcttgaa
     3841 tttgcaatag ctgtgttcag aacggtcaat accgctccta ttatcatttg gtcggtcagt
     3901 cgatattcat atttaaagca atcgggattt ccgcagttcc aatattcacg tcccttgccg
     3961 ttggtttttc tgaaaagctt ttctccgcat tcaaggcagt atgtaacctt tctgatttcc
     4021 tgtaaatcat cgcataccag attaagcgta ttagcctttc ttatccgctt ttcatttgcc
     4081 tgattgaaaa tatcctcatc aattatctgt ggatatttgt cagttccgag atgtttctca
     4141 ttttcgatta tccgcttgac catgttcttg ttccaatggt cagaatcctc agtatatcgt
     4201 atttttttag actccattag ctttgcgatt tgaagtaaac tgctgccgtt cagatattcg
     4261 ctgaaaattt ccgcaactgc gcaaacttct ttcggttctg tagttatttc gccgcttttc
     4321 atgcagtagc cgaacggtat tactctgttt ttgggcatct aatttcacct ccatgtacaa
     4381 caatatcaca aacttctgcg gaaatccagt accaaaaccg acaaaaatat gccctgcaat
     4441 tttactgcaa ggcactttcc gttatatgtt ttcttttaac ttcaatccgc cgatcaaatg
     4501 aaactccagt tcatgctgat ctgtagcaac aattttctca actatactct caaatgccaa
     4561 ttcttcaaac tcggttatcg gattatctcg cttttcaaaa aaatcaatca acatttcaat
     4621 ctgatcaagc gtttcattct catcgtcgga acgtgtaagc tttttcagct cggattgcag
     4681 tttattgatt ttagctgtaa gctccgtagt ctgctcaatg tattttgcct cgtccagaaa
     4741 tccttttgtt ttcagccttg cgagtacatg tgtctgctcc ttaagttttg cgatttcttt
     4801 gtgaatatcc ataacattgg tgtttccgct gaattttttc agctttaaat cctgtaacgc
     4861 cgtttgacag gggagaagta tctgtttata gttgtaccac agcttattgc ataatgttat
     4921 aaatgccgag atgacctttt cttctaaaat ctgcttattt gaacatctat cagactctga
     4981 gtcatgcagt cggcataccc aataattttt attttttctg catttaaact taaatgttga
     5041 accgcactct ccgcagtaaa tcttctttgc aaatatatga tcttgcttca atcctttaaa
     5101 cgatttctta tgctccgcta aaatattctg aaccttatca aaatcatctc ttgaaattat
     5161 gggcttgtgc ctgtcggaat aatagtattg atctcgttcg cctttattta ctacctgtcg
     5221 aaacggaagt gtatctgtag tatacttttt ctggaacagc tgatcaccta tatacttttc
     5281 atttctaagc ataaccccaa ccgtacttgc tgtccaatga cttgttctgc cgaacctttc
     5341 tattttgaga gcattcagca tttgggctat agccgccaaa ccatatccat tcagatacca
     5401 atcaaatatc tgctgtacta tttcagcctc atcagatttt aaaatcaaat ttccgtcaac
     5461 aagttcgtat ccgaaaactg gagtacacat ttttattgtg ccgttctcca ttctcttttt
     5521 tataccccaa cgcaagttct gcgagatcga agttgattcc tcctgcgcca gaccgcccat
     5581 aattgtaatc atcatttcat cggtcatatt ggcagtatca atgttttctt tctcaaaata
     5641 tattgtgata ccaagcgatt taagctctct gacgtttttc agacaatccc ttgtgtttcg
     5701 ggcaaaacgg cttatggact ttgtataaat tctgtcaatt tttccttttc ggcaatcctt
     5761 cataagtctc tgaaactcgt cacgcttgtc ctcacgggtt cctgtaacac cttcgtcagc
     5821 atatatgtcg atgagttctt ctgtttccga atcctcaaaa atctgactgt agt
//
LOCUS       DAWEMR010000160         5825 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5355_length_5825_cov_11.410225, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000160 DAWEMR010000000
VERSION     DAWEMR010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5355_length_5825_cov_11.410225"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1888 a          841 c         1398 g         1698 t
ORIGIN      
        1 aataagtgta tagtgtatag tttatattac atttattgct gatgcatttt agttgtgaaa
       61 gaactcgaag tataaggaag tttaagaaaa tgaacagaaa gatagcagta gcaggaacag
      121 gttatgttgg attgtcgctt gcagtattgc ttgcacagca caatcatgta acggctgttg
      181 acattatccc tgaaaaggtt gagcttatca ataataaaaa gtctcctatt caggacgatt
      241 atatagaaaa gtatcttgct gaaaaagagc ttgatctggc ggcaacgctt gacggcgaaa
      301 cggcgtacaa ggatgcagaa ttcgtaataa ttgcagctcc gactaactat gacagcaaga
      361 agaatttctt tgatacttca gctgtagaag cagttataga gcttgttatg aaagtcaatc
      421 ctgaggctat catggtcata aaaagtacta tcccagttgg ctacacaaag tcagtaaggg
      481 aaaagtataa tactgacaag atcattttca gccctgaatt tcttcgtgag tcaaaggctc
      541 tgtatgataa tttgtatcca agcagaatta ttgtaagctg tgatgatgat acaagagaaa
      601 aggctgagct ttttgcaaag cttttgcagg agggtgcaat aaaggaagat attgacacgc
      661 tctttatggg atttaccgag gctgaggctg ttaagctgtt tgcaaatact taccttgcac
      721 tgagagtttc gtattttaat gagcttgata cttatgcaga ggtaaaggga cttaatacac
      781 aggatattat aaatggtgtt tgtcttgatc ctagaattgg tagccattac aacaatccaa
      841 gctttggtta cggcggatat tgcctgccaa aggatacaaa gcagctgctt gcaaactatg
      901 aagatgttcc acagaacatg atgtctgcta ttgttgagag caataggact agaaaagatt
      961 tcattgcgga taaggtgctt gagcttgcag gctattatac cggaagcagt aattgggata
     1021 aggatagaga aaaggacgtt attataggcg tttataggct tacaatgaag tccaactcag
     1081 ataatttccg tcagagtagt attcagggcg ttatgaagcg tatcaaggca aagggtgcta
     1141 cggttataat ttatgagcca acccttgagg acggagatac gttctttgga agcaaggttg
     1201 taaatgattt ggaaaagttc aagtcaatga gccagtctat tgttgccaac agatatgaca
     1261 gctgtcttga tgatgttaag gataaggtct atactagaga tctgtatgga agagattaat
     1321 tagctaggag cttgatttat gtacaataac aaagatgatc aggaaccctt taaacaaaaa
     1381 gtatggcttg cgtcacctac aatgcatggt gaggagcttg aatacgttac tgaggcttat
     1441 aatacaaact ggatgtctac agtcggtaag aatataaatg aggtcgagaa gatagcggcg
     1501 gagaagtctg aagtcaagta tgctgttggt ctttcatgct gcacggctgc gcttcacttg
     1561 tgctgtaagc ttgctggcga gaggctttat ggcaagcctg ctatatctca tggcgctctt
     1621 gagggcagaa gagttttttg cagtgacatg acctttgacg ctactctcaa cccagttgtt
     1681 tacgagggcg gtatccctgt ttttatcgac actgacagag atacttggaa catggatcct
     1741 gtggcgcttg aaaaggcttt tgagatgtat cctgaagtca agctggtagt aagcgctgag
     1801 ttatacggtt ttcctggaag aatggacgag ataaagaaga tatgcgagaa gcatggtgcg
     1861 cttttgatcg aggacgctgc tgaagctatg ggtgctacaa ttgacggcag acagtgtgga
     1921 agctttggcg attacagtgc tgtaagctac aatggcaata agataataac aggttctagc
     1981 ggcggatgtt tgctgacgaa cagccttgaa gatgcaaaca gggctcgtaa gtggtcaacg
     2041 caggctcgtg agaatgcggc atggtatcag catgaagagg tcgggtacaa ctatcgtatg
     2101 tcgaatgtta ttgcaggagt cgttcgtggt cagtatccgc atcttgagga gcatattgca
     2161 cagaaaaaat caatatacga gagatacaaa gagggcctta aagggcttcc tgttaaaatg
     2221 aatcctatcg ttgagggtac agagccgaat tattggttgt ctgcacttat catagataaa
     2281 gacgcaatgt gcaagcaggt tagaaatgac agcgaggctt tgtacatcaa agaagctggc
     2341 aagacttgtc ctactgaaat tttggaagcg atagcaagca taaatgcaga gggaagacca
     2401 atatggaagc caatgcacat gcagcctatg tacagaatgc acgagtgcgt tggacgtgct
     2461 ggcagtatca gatgccgcac aaatgcttat attgcaggcg gtgcagaaga tgtaggcgct
     2521 gatatctttg acagaggcgc ttgcctgcct agtgataata agatgactga ggaacagcag
     2581 gagagaatta tttgggctat caggagttgt tttgagtaat gtatgcgaaa tacataaaga
     2641 gaatacttga ctttgttttg agtttaatgg cacttatcgt gctgtctccg ctgttggtga
     2701 tattgataat tcttggtgcg gtgtttatga ggggcaaccc gttttttact caggcacgac
     2761 cgggtaagga tgagaagatc tttaaactca taaagtttcg taccatggac aatcgcaagg
     2821 acaaggacgg aaagcttttg cctgatgatg tgcgtttgaa taagtatgga aggatactta
     2881 ggagtacttc gcttgacgaa ttgccagagc ttatcaatat tttgataggg gatatgagca
     2941 ttgtgggccc gaggccgcta atggcaaggt atcttgatta ttatactgct gaagaatctc
     3001 gaaggcatga agttagacca ggtttaacag gttatgctca ggttcatgga agaaataatg
     3061 ttaattggga tgaaagaatt aaattagata tagtttatgt agataatatt tcttttgtta
     3121 tggatttaaa ggtttttatt gatacagtcc tcatagttct taaaagagaa ggcataggca
     3181 cagaaggtat gagtaatttt gatgattata gaaaaaaaca gtgggaaagt caaaaacaag
     3241 gtgatcagca tgaattataa aacgccatgt tatgtaatac acgagaagga attagacgac
     3301 aattttatta aattgaccaa tgcattacaa aagcattgga ataactatat tataggttat
     3361 tcgtataaaa ccaatgctct tccgtggatt ataaaacatt ttaatgattt gggatgctat
     3421 gccgaaacgg tatcagaaga tgaatataat ttagcaaagt taataggtgt gtctaaagat
     3481 aagatcgttt ataatggacc aattaaaaca aaagatacat ttatagaagc ggttaataat
     3541 aattgcattg ttaatattga ttccgagaga gaaatcgaat ggcttgatga agtggatata
     3601 cctatgcgtt ccgttggaat tcgtgttaat tttaatattg agaaaatgtg caaaggtcag
     3661 tcacaatgtc cagaagatgg tggccgtttt ggattttgct atgaaaacgg tgaattagga
     3721 aagactatag atattttact tagtaaaaaa gtgagaatat ccggattgca ccttcatact
     3781 agttcaaaat caagaggatt agatatttat agggcaattg cagaagttgc atgtagaatt
     3841 cagaaagagt atagccttga gttagattat gtagacgttg gtggaggttt ttttggcggt
     3901 ttgccaacaa agccgcagtt tgatgaatat atatctttaa tggaatctat tttaataaaa
     3961 tgctttaata aacaaaaaac aaaacttatt gttgaaccag gcatggctgt tattggagct
     4021 ccagtaagct atatcactac ggtgattgat gttaaggata cagaatttaa tagatttgtt
     4081 attacagatg gaacgaggac aagtatcgat cctttgatga caaaaaaagc ttattttcat
     4141 agattgaaaa taaacggaga aagatatgtg catccgaagc aagttgtttg tggctatacg
     4201 tgtatggaac acgatcgcct gtttgaagaa ttagaaggtc ctactttgca gatgggtgat
     4261 caaataattt atgacaaagt tggtgcatat actatgtccc ttacaccttt gtttataaaa
     4321 tattttcctg atgtttatgt ggaaaataac gggaaatatg atttggttag aaaagcctgg
     4381 gagccagagc agtatgtaca gttatctaag tattaaataa tattggtgag gggaaatgaa
     4441 ttatgaagaa tgctatagtg tttggtgcaa caggccagtt gggatgttat agtgcacttg
     4501 ccttaaaaga aaatggatac aatgtatttg caataggaag aagagcaagt gatggcggct
     4561 tttttgctac taaaggtatt tcctttattg gtggtgtaga tgtttctgac atatcaacat
     4621 tttcaaagtt gccaaaagga aaaatagatg tcgttgttaa tatggcaggc acaatgccgg
     4681 cgcatgctga cagagatatg atgccatatg tacagagtat agtagttggg acagttaatg
     4741 tatgtaattg gatgaagaca gttgactgtc aaagaattat ttttaatacc acacctagtg
     4801 atactgctga atattggggt acaaccacac ctattgatga cgatgttgtc agatcatttc
     4861 cgaaaaacgg aaatgatcat gcagtctatg caatatgcaa aagagctgct actgatattt
     4921 tagaacacct tcaaatatgc ggtgaggtaa aaccatgtgt gtttagacat tttatggtct
     4981 atgggtggca tccagatgcc acatattatt tggagggcaa agagaagata ttgccatggc
     5041 gttctatgat aagaaagtgt atacagggcg aaccacttga agtatatggt gatgtgacaa
     5101 gaaaaaagga attactgtat ataaaggatt ttgcaagggc ggtagtaatt gcagctaata
     5161 atgatgcatg tggtattttt aacctccccg gatacgaacc atattcttta gattctatgg
     5221 tcgatggaat aataaatgct ttttctgacg gacgaacagt aaaaaaatat agaccagata
     5281 tgccagatac accgcaaatt cttttagctc atgaaaaaag taaaaatatt ttgggatggg
     5341 aacctatttg gacttgggaa aaagcgtgtt ttgacatgag aaaagaatgt attgaaaacc
     5401 cagttaaatt gatgtggggt aataatgatc cgttggacca aattaatgga gaagcttagg
     5461 atgaatattt tatttttaag tgcagggaca agaaacaagg ttgtgcagta ctttgttaaa
     5521 actttagatg gtaaaggtaa agtcattgct acagatatga gtaatcttgc accagcaatt
     5581 tatgatgcgg ataagtacta tattgttcct cgaatgaccg aagagggtta tatagatgtg
     5641 attcttgaca tttgtaaaaa agaaaagata agtggtgtac tttcacttat agatcctgaa
     5701 ctttcacttc ttgcagagaa taaagaaaat tttgaagctg taggaacaaa ggtaataggt
     5761 tcttcgtacg aactttgtga gatgtctctt gataaatata caatgtataa ttggctgact
     5821 gatca
//
LOCUS       DAWEMR010000161         5806 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5365_length_5806_cov_17.380282, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000161 DAWEMR010000000
VERSION     DAWEMR010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5365_length_5806_cov_17.380282"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1911 a         1028 c         1317 g         1550 t
ORIGIN      
        1 atataatgat attgtagtga ggtgaaagat tatggataaa acaataattt atcaaggaca
       61 gattagtgga tttccattgt ttaaatttca gaccgcagat ataattgaaa aaatacaaaa
      121 aggttcattt tatatgaata gcttaaaggt atatagagat cgatatcaaa ctagtggaga
      181 tgaagaaatc ggagatcctt ttgagggaaa aatttatgtt aataacgctc aattgattat
      241 cccggaaaag agtataattg aaccatgtaa taatcaagtt ttttcaactc caaatgaaga
      301 tgattttgta ttttgtatgt ttgggatcaa tccgcaaatt cataaatcat tttgttttaa
      361 tgaagatcaa aagaaaaaat ggttagaaat ttatgataca gcgttaatta ttaaggatca
      421 acaagagttt tttaatcgaa taaaaaataa agctttagaa atgaatattg atattatagg
      481 tgactttgtg aattattacg atgattcaat aaacgatgta accccattta tatgtagctt
      541 attaaaaggc atacgaaatt cagtatttca taaaaggaaa aaatacgcat accaacaaga
      601 atatcgattt actatggtta ataataaaaa atcagataac tttgaaatga atataggaga
      661 cattagtgat atatcaacta tacttccttt ggataaattt ttaaatgtag aaatctatcc
      721 tcacgagtaa ttattgtaaa tacagtcaag ccttgatata atccccttag agtagacaca
      781 cgaaaaaaca aaaatcgtga aaaaactcta agggggtatt ttatgtcaag aagaggaata
      841 ttgagtgatg aggaaagaat tgaagcggta caggaatatc ttgaagggaa aggcagctat
      901 cgctcaattg caagaaaata tggaatcaat tacgaacgat ttcgacaact tgctatccgt
      961 gcgaaaacag aaggtataga agcggtcaaa acgaggagta caaacaaaaa atatcaagtg
     1021 gaaacaaaaa taaaagctgt gagagaatac atggaaggta aatatagcct tgtagagata
     1081 tgttcaaggt accaaattag cggtcacagt gttctcatcc aatggataaa gtggtataat
     1141 agtggtagag actttaagga gcgcacacgc tcggaaagag gaattaccat gaacaaagaa
     1201 agaaaaacca cacaggaaga acgagcagag atcgtagcat tctgtattga aaacggcaag
     1261 gactatagac ttgcgatgga gaaatatagt gtatcctatc aacagatcta ttcctgggta
     1321 cgaaagtatg aagcaacggg catagaaggt ctcaccgacc gcagaggcaa agcaaagccg
     1381 gaagataagc ttacagaagc ggacaggctg cgaatggaaa acaagatatt acaagccaag
     1441 ctgaaagata tggaaatgga gaataagctg ctaaaaaaac tcagggaact gcaaggaggt
     1501 ggtcactgag cggagtacgg aacgaggaca agtatcttgc aatcaaatac atgaatgagg
     1561 cggagcatta cccgattcaa aagctctgtg ctgctgttca catcgcacac tctgcatatt
     1621 acaaatggct gaaaagaacg ccgagtttaa gccagaaagt caatgagcag ctggcagaat
     1681 ggatcaaaca gcactatgag gaacgaaacg ggatcttagg atatcgtcag atgacggtcg
     1741 tgataaaccg tgaacatgat gtacatttca acaagaagcg tattcacaga ataatgcaga
     1801 tccttcatct gagatcagtg actcgtatca agaagaatac atatattccg tcaacacctc
     1861 agatcacagc agagaatgtt ttgaatcgag attttcacgc agataagcca aacgagaagt
     1921 ggctgactga tgtaacggag ttcaaatatt acattggcat gaatgttaaa aagctgtatc
     1981 ttagtgcgat cctcgacctt tatgacaagc gtatcgtggc ttacaagatc ggcagcagca
     2041 acaacaatga tcttgtgttt tctacatttg atgaagcagt tcatctgaat cctgacgcgc
     2101 atcccatctt tcactgcgac agaggatttc aatacaccaa caagatgttt catcaaaaaa
     2161 ttgttgatgc cggaatgata cagagtatgt cacgtgtcgg aagatgcatt gataatggtc
     2221 caatggaagg ctggtgggga atcctcaaat ccgagatgta ctatctccgc aagtttacag
     2281 ataaggatga actgagctcg gcgatagaag aatacatcag ataatacaat acaaagcgtt
     2341 accagatcaa gctgaattgt atgacaccga tggaatacca tgaagcgtat gctgcttgat
     2401 cgaacgtcgg gtgcttgcca attgctaaca acaaagcagc ctgcacaaat tgcacagact
     2461 gcttttagtc attacatgga atatttttgt tttttgcgct gtctacttga cagggggcgg
     2521 gtcacctaaa aacttggctg tatttttata cccaaaagga ggtttgataa aattgaaaac
     2581 ctacatctac cccgaaaacc tgagagcaag cgtaaagctg tggttttgga gcgttcggga
     2641 ttttatcata atctgcggcg gaataattct gtcggttgtg attctggtga acttctggaa
     2701 cgtcctgccc tttgctgcga ccgcctgctt tgcgtttctc tctcttcggg tcgacgaaac
     2761 tgcaattata gactatattt ttaacgctgt gaagtttttt ctgacttcgc agcaattgta
     2821 tttatggagg aaagattaaa tgacaaagaa caaaaacagc gttcagggct tgataggtct
     2881 tgaacgcttt actcgtttcg gtgtaaagac cgacaaagcc gagcttgctt ttttcaacgt
     2941 agaacctacc aatatttctg ttctgtcagc agctaatatc gatgtcaaaa tacatcatct
     3001 tatgatgtta ttgagtacca tacctgacct agaaatactg gcattggact cctgcgagtg
     3061 ttttgacagc aataaaaatc atcttagaaa aaggcttgaa aaagagcaga acgaggcggt
     3121 cagaaaactg ctgcaagctg actatgattt tcttgatgag atacagattg agatgtccac
     3181 ggcaaggcag tttatgtttg cggtgcgttt cagacgtgaa aaggacgagc agatattcag
     3241 cacgctgaat cgtgtggata aggcaatatc ggagcacggt tttgcggcta ggagaatgag
     3301 taagccggag atcaagcgta tgctggcttt gtatttcggg acaagcatat ccggagatga
     3361 tatcccagat attgagggag agaatgaatt tgatctggaa aaacaggagg tgactgttaa
     3421 tgagaagtag aaagcaaaag aaaaaagaac tgtcagaaga acagcttgag atcatcgata
     3481 caaaggactt ttttgaccgc atttcaccgg gcattatacg cttttacaca gatcactaca
     3541 tctgcggaaa cagtttcaga tgcgtctggg ctgtgactga gtatccgccg agcacagaag
     3601 aaactgctat tcttgctcac cttgcggaca gaaacggcgt tacgctgagg atatatgaca
     3661 gacttgttac cgccgcagaa cagcgaaaga tcgtgcagca ggcaatgagg aataatcata
     3721 tgatgacgac tgtaaatgat gtgaacgaga gcataaaggc tcaggataac ataaatgatg
     3781 tggtggagct gataagcgaa ctacgcagaa acaaagaacc tttactgcac acagctgttt
     3841 ttattgagct aaaagcaagc tctgaggaca agctgaaaga actgcaagcc gatattcaaa
     3901 tggagcttac acgttccaaa atatccgttg acagactgct gttacgacaa aaagaggggt
     3961 tcttgtctgt tcatcctgcg ggcaataacg tgtttgcaag tcagtttgaa cgtgttcttc
     4021 ctgcaagttc tgtggctgat ctttatccgc tgaactattc aggtaagact gacgaaaaag
     4081 gcatatatat cggccgtgac aagtttggaa ctaacattct tgttgacttt gacaagcgaa
     4141 ctgaggacaa aaccaactcg aacattttga tacttggcaa cagcggtcag ggcaagtctt
     4201 atcttatgaa actgctgttg tgcaatcagc gtgaatcagg caaatctata ctgtgcttag
     4261 accctgaaca cgaatatgag gatctgtgca ataaccttgg cgacacatat attgatatga
     4321 tgtcgggcga gtttatgata aatccgcttg aacctaaagc gtggtcagaa aattctcgcc
     4381 ctgaaaatca agaaaaggaa accggtgaca gccctgaaac attccgaaaa gtaacccgtt
     4441 taagtcagca tatcagctat ctaaaagact ttttcagagc atacaaagac tttacagacg
     4501 cagagatcga cacaatagag ataatgctta tgaagctgta tgcacgtttt ggcattgacg
     4561 atcttacaga ctttgacacg ctgatagatg aggattatcc gatgatgtcc gatctatacg
     4621 atcttgtgga aaaggagttt tacgcttttg ataagtctaa aaagcagctt tacacagaag
     4681 aaatgttaca aaatatctgc cttggactgc attctatgtg caagggtgca gagtcaaaat
     4741 attttaacgg acacactaat atcaaagacg ctgagtttat ctgtttcgga gtgaaaggtc
     4801 ttatggacac taacaaacgg ctgaaagata ctctgctgtt taacatcttg tcatatatgt
     4861 ctgatcagct tttaggcaag ggaaatactg ttgcggcagt tgatgagctg tatttattcc
     4921 tcactaatat gacggcgata gaatatattc gtaacggaat gaaacgtgtt cgaaaaaagg
     4981 aatcgtcttt catattagct tcgcaaaata tcgaggactt tctgctgcct gagataaagg
     5041 aattcacaaa gccgctgttt tctattcctg cacatcattt tctgttcaat ccgggaaaca
     5101 tctcgccgac ggcatttatt gacaccttac agttggaaga atcagagtac agtcttatca
     5161 agtatcccga acgaggtact tgcctttaca ggtgcggtaa tgagaggtat ctgctgcagg
     5221 tcattgctcc tgcttacaag gcggcgctgt ttggttcggc gggaggaagg tgatgtaatg
     5281 tcggcgacag ttgcggcggc tcttaaaaag gcagccgtat atattcttgg tgatgaaaaa
     5341 aagcgaggca agcttgtgac tgtggtgctt agtgctgcgg tgggtctgct ccttttgttg
     5401 tgtgctcctg cggtgatgct ttcttctctt ggggaggtcg gcgaggaagc tcaagtgcct
     5461 gaacttcaca ttaatgccga tgatttttac agctctcttg acagtgaaag caggcaaagg
     5521 ctcgatgata tccgatcagc aggtaatgac attgcaaacg ctatggacga aaagggtgtc
     5581 aaggttcaga ccataaaggc tcaactgata tatatgtctt actttgaaga tgttcagaat
     5641 tttaatgctg agtcatacgc aaatttgttc gctgttgctc caaatgacag tgatttaatt
     5701 gcggcgataa actcgactta cggtttggat attgactatg atgagtttgt ccgcacatac
     5761 acttttgtga tgaacagcac cataaatgcg tttatgttta ctgata
//
LOCUS       DAWEMR010000162         5805 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5366_length_5805_cov_11.816348, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000162 DAWEMR010000000
VERSION     DAWEMR010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5366_length_5805_cov_11.816348"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5202..5211
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1766 a         1139 c         1215 g         1675 t
ORIGIN      
        1 aaaatgacat ttatcccata atatataaca tctacactta tgatgacaca gaaacgatcc
       61 gtattcttct cgattatgat gatattctgc acatacttat gccatacgac gctaagaacg
      121 tatgccacta tatgcttgac aagctaaaaa ggatacctgt tgatcctgat attcttaaac
      181 agaatatttc aaaggagata cataggatca taacatcaac gggcataaac cgcaagcatt
      241 caggatatga ccatatctac tacatgatat tccttattat attcaaatat agcggtaata
      301 aagtgcaaat aggcgaactt tataaggaca ttgaaaaaca atacggaaaa aatactgcgg
      361 ctattgaacg ctcaacaaga agtgctatag tttcaggctg ggaaaaaatg aagcctgtgg
      421 ataagcaaat gctttttgaa agttgccttg ctaacggaac aaagcctaca aatgcaatgt
      481 acataaacac tatagcttta tatataaagc atttgtataa cgatcagttg gagatgtatt
      541 acaaaaacaa aaacgatcat acataggacg atccccaaag tgcataccta ttataaaggg
      601 tatgcacttt ttttgaaagg ggaaggtttc atgatctgta gtttttcaag cttacgaaat
      661 aaagaggtcg tcaatatgcg aacaggactg aaaataggct atgttgatga tatagaaatg
      721 gatactcagg gagcgaacat agtttcactg atcgtctatg gcaaaccaag ggctttcggt
      781 atcatgggca gagatgagga tattatcata aaatgctcgg atatcgagct tatcggcgaa
      841 gataccatac ttgtaaaatt caacgatagt gcagtatgca caaagagcag aactgtaaaa
      901 atcgaaaatt tgttaaaata aattgtcgaa agctcttgaa atgaaataga aaatatgata
      961 taatattaaa gcaattcgca cggacaggtt tttgccgttg gtcctgccgt aagacaggtg
     1021 aaagcaagta aagccgtccg gaggataaaa acatctgcat aagcagaaat ctaaaaccaa
     1081 acaggaggtt actacaatgg gagtagtatc aatgaagcag cttcttgaag ctggtgtaca
     1141 ctttggtcac cagacaagaa gatggaaccc taaaatggct ccatacattt tcacagaaag
     1201 aaacggaatt tacatcatcg atcttcagaa gaccgttaag aagcttgaag aagcttatat
     1261 gtttatccgt gacatctcag ctaatggcga agaggtactt ttcgtaggta caaagaagca
     1321 ggctggagat tctatcaagg aagaggcact gagagctaat gcacattatg taaatgcaag
     1381 atggctcggc ggtatgatga caaactacaa gacgatccag cagagaatca agagacttga
     1441 gcagctccac acaatggaag aggacggcac attcgacctt ctccctaaga aggaagttgt
     1501 taagctgaag cttgagatag aaaagcttga aaagttcatg ggcggtatca agaacatgaa
     1561 gaagcttcca ggcgcacttt tcgttgttga cccaagaaag gaaaagatcg ctgtagcaga
     1621 ggctaagaag cttggcatac ctgtagtagc tatcgttgac acaaactgcg atcctgacga
     1681 tgttgattat gttatcccag gtaacgatga cgctatcaga gcagttaagc ttatcgctgg
     1741 ctgcatggct aacgctataa tcgaaggcag acagggtgag gacgctgagt ccgctgaaga
     1801 ggcagataag gtggaagaag ctgacgaggc tgctgaataa gagctgaaat ttcaggcagg
     1861 catttatagt atatttgtct gcctatttcg atataaaaca ctaggaggaa taataatggg
     1921 taaggtatcc gtagcagaga tcaaagaact tatgacagca acaggcgttg gcatgatgga
     1981 ctgcaagaag gctcttgtag aagctgacgg cgatatggaa aaggcagtaa cacttctccg
     2041 tgaaaaggga cttgctacac aggccaagaa gagcggcaga gttgcagcag agggtatctg
     2101 cacagctgta gttaagggca acgtaggctg cgttcttgaa gtaaacatcg aaacagactt
     2161 tgctgctaac aatgatgagt tcaaggcttt cgttgcagca gttgctgata cagtaatcga
     2221 gacaaaccct gctgatgttg acgcacttaa ggcaacaaag atcagcggtg gtgacaagac
     2281 agttgaagag acacttcagg atctgttcat caagatcaga gagaacatgg taatcagaag
     2341 attcaagaga atggaaggca ttcttgttcc ttacgttcac ggtgcaggca gcatcggcgt
     2401 tatggtaaag cttgattctg acattgcagc tgacaatgct gagctccttg ctgctggtaa
     2461 ggactgtgca cttcaggttg cagctatgaa tcctgcatac ctgaacagag aggctgttcc
     2521 tgctgacgct cttgacaagg agagagagat catgctggct cagatggctg aagatcctaa
     2581 gatggctaac aagcctgagc aggttaagca caagatcatc gacggtaaga ttggcaaata
     2641 ctattctgag aactgcctcc ttgagcaggc attcgttaag gatgataagg tatctgttgg
     2701 caagtacgtt gagtctgtag ctaagaagct tggcggcaag atcgacgttg ttgagtacgt
     2761 tcgtttcgag agaggcgaag gcgtagagaa gaaggaagag aacttcgctg acgaagttgc
     2821 agcaatgaca aagtaatctt tctgacataa atgaaagtcc ctcggtggtg agccgaggga
     2881 cttttttatg ggcttttgat tgtaacttgt caagttttac tataatacat aaatatatca
     2941 aaaacagatt tacataaaaa gatatgagct tgaaaactca ttttttcaga atcttttcat
     3001 ttactaatct tattcatggt tccgaaaaat aaaccgtgta aacgcaataa tcgccaaatt
     3061 tctgacaata ctaaaattgc agccaaacag ggcaacgctg aggaacgacc tgacgcaggc
     3121 tgtgagataa aacggtggaa agcggcggca gaaatgtcgt cggttttcaa tattccaccg
     3181 gatatttcca aagcggataa acctcagggg tttggggaca gagtccccga cagccgttag
     3241 gctgcaaatc tgtcctcaaa gtatatcgca aactgagaat acgccagacc ccagtctctg
     3301 ataggcatgg tccacttctt ggctatctct ttgacgctca tatagatgac cttgcgtatg
     3361 gaatcatcgg tcggaaaaac cgcctttgat tttgtaaatt ttcttaccat tctatggtag
     3421 ctttcgacag cgttcgtcgt atatattatc ttcctgatct cctgcggata ttcaaagaat
     3481 gttgtcagtt cagcccagtt tttgtcccat gattttatga tgttgggata tttcttgtcc
     3541 cacttttcac gaaactcttc cttggcaaat tcagcgtcgt caagattgac tgcaccgtag
     3601 attttcttta aatcagcaca cacagctttt ctgtccttgt aaggcacgaa tttacagctg
     3661 tttcttatct gatgaacgat gcagagctga ttctttgtct tcgggaaaat agcgttgatc
     3721 gcatcacaaa gtcctgtcag attatcgtga caagcgatga aaatgtccgt gactccgcgg
     3781 tttttaagat ctgagcatat acttgcgtaa aagcttgctg actcattttc agaaatccat
     3841 gttccaagaa tctccttctg accctccatg ttgataccga gtacagagta cacgcactta
     3901 ttgatgattt tattgtcctt gcgcgaatta aaaacgattc catcaaagaa aacgacagga
     3961 taaatactgt caagaggtct gttctgccac tcgtttactt cgggaaggat cttgtcggtt
     4021 attcgtgaaa caagtccttg agatatttca gcaccataaa tctctttgag ggtatcttcg
     4081 atgtcacgct gagacattcc cttagcgtac atcgccatga tcttctgctc aatttcgtcc
     4141 gtgtgagtct gtcttttttc gatgatctta ggttcaaatt caccgtttct gtcacgggga
     4201 acggctattt cacactcgcc gtaatcgctg gaaatggtct ttttgccgta accatttcta
     4261 ctgtttccgg agttgttgcc tgcggcagaa tgcttctcat aacccagatg ctcgtccatc
     4321 tcagcttcaa gcatctgctc gatagttcct gcaaacaaac gcttgagttt agcctgaata
     4381 tcgcctgtgc tttcgcactc cgccatgatg agcttcacaa gctccatttc cttctcgctt
     4441 aaaccgtgat ctcttgtttt cattacaatc tacctccaaa tttctttgtg ggtattattg
     4501 tcatttacac ggttgggtat tcagactctt attcatctcc caattctgga ataccgccaa
     4561 atttaccaat tagatagcca gttcttatat cagcgttgct ttttttaccg agcccatcaa
     4621 cactgtatag ttcagactcc cctgtttgtc ggtctttatc tacgaagtaa aattggtcag
     4681 catcaagaaa atgttgtctc attatttcgg tatcatgggt agtgaatata agctgagcgt
     4741 gatttttatt gatttcagaa ttttgaaatt tattgactat atactccgtt agtaacggat
     4801 gaagtccttt ttccagttca tctgcgacaa atatgccacc attatcaatc accctgtcaa
     4861 ttgccggagc cagtgccata agcctatgag ttccatctga ctcatcttct aaactaagct
     4921 catattgacc tagctctcct tttttgttta caccagtatg ataggttcga gccttaattt
     4981 catttctttg tatttttgcc tgagaattat cagacaacgc actaacaaaa cttttcagag
     5041 ctcttgacaa ctcaggagga gcgttttccg gtatggtgtc atcaacatct atctccgtgt
     5101 ttttcatatc aaacttcata tcaattatac caacatctgc acgttgagca tattgtttca
     5161 tggattttag aacattgtta ttgttataat taaatctgat cnnnnnnnnn ntagactgaa
     5221 agaaaattct tgtcccgtcc ttttaaaaac ttgagcctta gaacccttgg gataatgaaa
     5281 aagatattcc tcgcatatct tctctttcgt cgcagaaaag ccatagatat actttattcc
     5341 attatatttg tatataaatt cgaaacttgt cggagtttcc gtagaatagt cattcaattt
     5401 aaaaggatca acgggcacag gcgctccagc accttgtgtc atttgtgcat tgcatataaa
     5461 tttaacggca agccaaaatg ctcttattaa attacttttg cctccaccgt ttttaccata
     5521 tatagccaca agtggcagag attctttttt cttaattaaa ctctctaacg ataatgtgtt
     5581 ttcccctcga aaagagcggt aatttgaaaa cgaaaattca attaacatat atcttttaca
     5641 ccttttcctt ttataaaatt caaacgggat tattgattat tattggtgct tttatattat
     5701 tataacattt taattttaaa acatcaagct ttttgaagag caaaacagat tggaatgcag
     5761 cagttttggt tataggtatg agacaagaag gcatcctttg gggat
//
LOCUS       DAWEMR010000163         5779 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5395_length_5779_cov_9.758910, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000163 DAWEMR010000000
VERSION     DAWEMR010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5395_length_5779_cov_9.758910"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1816 a         1027 c         1438 g         1498 t
ORIGIN      
        1 cacgcatggg cggcaacaaa gcttggggat gatacccctc gttatcaggg tagactttcc
       61 cttaaccctc ttgcacatct tgaccctatt ggtgcattgt gcatattctt cataggtttt
      121 ggttggggta aacctgttca ggtaaaccct atcaggttta agaattacag gaagggcatg
      181 gcactcacgg cagccgcagg tcctatctca aatatcataa tgggacttat cggaatagtt
      241 ctttataagc ttttctatgt tctttatata gtgcattatt ctatagctct ttactatgtg
      301 tcgcttatat tatattattt tacagctata aacgtgggac ttgcagtgtt caaccttata
      361 cctatcccgc cgcttgatgg tcagaagata ctttcttact ttacaagcag taaggtagat
      421 cgctttatgg cgcagtatca gaactatatc gctattgcat ttatgatact tgttgtaaca
      481 ggcgttcttg atacgcctat aggttggttg caaaatggta tgttctggat aatggataaa
      541 cttacgttct gggttgaccc tatactaaaa gcgatatttt tgaaataaaa ctgttcatga
      601 aaggaaaaac gtaaatgagt gctgctcagt tcaagctgga tatgtttgaa ggaccgcttg
      661 acttgcttct tcaccttatc agcaagcaca aactgaatat ttacgatatt gagatatctt
      721 tgctccttga acagtatatg gactatatca atgatcttga ccacgaggat tatgaggatg
      781 ccgctgattt tcttgaaatg gcggcacgcc ttatatacat aaagacctgt tcgcttctgc
      841 ctcatgacga ggagtcaaaa gagcttaaaa aagagcttga aggcaggatc atcgagtatt
      901 ctctgtgcaa acaggcggct ctcacgctcc gtgatgatta tgtgggcgat cttgtgttcg
      961 tcagagcagc cgtaaaactg cctgtaaata agacctatac ccgtgagcat gaccctcagt
     1021 gcctttatga cgcttatatg ggcatatcaa agaaagcccg tgagagcgtt ccgctgaaag
     1081 cgaagatgtt cgagccgata gtttcacata agatagtatc ggtcacatcg aagataatcc
     1141 atgtccttaa aagactttat aagcatggtg aatgcgatat gggcgacctt tatgacggca
     1201 tgacggagaa aagcgagaaa gtcgctactt ttcttgctat attggagctt acaaagtctg
     1261 gcaggatatt tctcaatgac gataactcaa agattttttt caacaaggct tcaaagaacg
     1321 gaaaagtcga gtccgacttc gataagcctg ttgttatcga aagcgaaacg acagaagctg
     1381 tccccgagcc tgagcctata gaaaatatac ttgacgatga gcctgcggat gagatagaag
     1441 aagttgctga tgttgacgca gagcgtgaag aagcttctca gtcaaagcgg tatatatctg
     1501 caaaatccga gcgcatatca ggtgaagtca ttgcggcgta tgctcctgtg aagtcagctg
     1561 aggaggtcat ggcagaaatg gagcagatat ctgaaaagat agacagtgtt gatgtatata
     1621 ccgagcctgt tatggcgaag acagagcccg aacagtctga tgaagccact atggaggaga
     1681 taaggctaat cgaacagctt gcggatatca cccctgatac agtattcaag ccgaactatt
     1741 gggcaaaaag aagatattat tggggatatt cacctgtggg cgatgacgga gacaataatt
     1801 tctggtgcta cggataaaat aagaaaaggg ataatctatg gaacattcaa atatacaggc
     1861 agccgtcgag gctgtgcttt ttgcaagcgg agagcctgtg gagagcgaga agctatgcga
     1921 ggcagttggt atcgtcaaag atcagcttga ggaagctctt ggctctttgg cggataaata
     1981 cagcaaggct gaaagcggaa ttacgcttct gagccttaat gggtcatatc agcttgccac
     2041 aaaagaagag cttgcgacat tcataaaggc ggctttggag ataaagaaaa actctgttct
     2101 tagccctgcg gcaatggagg cgctgacaat agtcgcatac aatcagcctg taacaaaagg
     2161 ctttgtcgaa cacgtcaggg gagttgactc ctcgggagtt gtaaattctc tcgtggataa
     2221 gggtttgctt gcagaagcgg gaagacttga cctgcctggc agacctattg catacaagac
     2281 gacggacaat tttctccgtt gtttcaggct gtcaagcctg gacgatctgc cgcctatccc
     2341 tgataagcag gagcagataa ccattgacga gataacaaaa gagaatgata cggaagaata
     2401 aagtatgacc atgaggggag atgataacga taattgtttt atggatactg cttggtatta
     2461 tagcagttat tgttatcctg cttcattttt ccattacggc acagatagat ctgaataaaa
     2521 aaggctttga tctaaaagtg cggtatatgt tttttgacat ttatccacga aagcctaaga
     2581 agaaaaaaca gaaaagatca aaaaaagctg aactgcctga ggatagtttt gaggattttg
     2641 aggagtttga ggacaatatt gaagaaagct tacctatccc acagactgat gatacgcctg
     2701 ataaaacagg agaagtgtcc gagcaaattg ttgaagctaa gcctgaaaaa atagaaaaga
     2761 atgaagcaga tgaaaatgtc agcgtggctg aggtagagga taagcaggaa gatatctctg
     2821 atattgatac tgatactaat gttgaagctg gtgcagagga caaaccagag aaaataactg
     2881 tgaaacagga gaaaaagggc tttgtcggaa agctttttca taaaaaggat aagggtgaaa
     2941 agaagccggt ttccaaatct aaagaaaaga aaaaaagcaa gttctatgag ctgaaagcgc
     3001 agtataaaaa ggtgaagcct tacattccaa tgggctggaa atatttcaaa aagcttctga
     3061 aagctatacg ctttacagac acaaggatag ttcttgacgt tgggaaagag gacgcttacg
     3121 aagcggctat gctatatggc aaggtgcagg cagggctttt caataccatt gcaatacttg
     3181 cgggtgtttt tacagtaaag cttaaaaaag ctgatgtaaa ctgcattttt gatgaaaaaa
     3241 agttcggata tgacgttcat accttagtca gggtaagacc aagcactttg atagcaatag
     3301 ggttttgcac gggcgttaat tttcttaaaa tatatctttc aggcagacgt aagaaaaagc
     3361 gtgctgccaa gcgtcgcagt aaagagcttg cggctaagaa gaatgaagtt aaagcaacaa
     3421 attgaaatac gaaaggttga gaaaaatgag cgaaagtaca aagatcgagg gacttgttaa
     3481 aactgcaatg gacaaggtcc gtgaaatggc agactgcgaa actgttgtgg gcaagcctat
     3541 cgttaccgca gatggtacta ctataatccc agtttcaaag atatcagtgg gctttgcgtc
     3601 gggtggctcc gacttgccaa caaaaagcac aaaggaaacg ttcggcggtg gctcaggcgg
     3661 aggagtaact atccagccta tcgcttttat cgctgtttac aagggcgaag caaagctttt
     3721 acagctttca atgaatgcga caaaggaaaa cgctatcata aatatggtac ctgaggtaat
     3781 agataagata acagattttc ttggcagaaa gtctgacgac gaggacgaag acgtggaata
     3841 attttactga attttaaaag cacaagtgag caaactaatc cttgaaattg attttaaggg
     3901 tgtggtttgt ttgaaatgcg tgacggtatt tatttgtacg gtcatgatga tgtgcttttg
     3961 ctgtatgaat gtcagcgctg acgatctcag tgagatatct gcgaagagtg ctatagttat
     4021 agacggtgaa acaggtgatg tgctgttcga gaaaaacgcc tatgaaaggc gtccgatggc
     4081 aagcactacc aaaataatgt ctgtgcttct tgtgcttgaa agtggtgaag atcttgacaa
     4141 gcagttcgtg gtagacagcg aagctataaa ggtcgaaggc tcgtccatgg gtcttcaaga
     4201 tggtgacaaa gtcactctac gcgatctatg ctgtggtatg cttcttccaa gcggaaatga
     4261 cgctgcaaat gctgctgcgg taagagtggc aggaagtatc ggcaagttcg ttcagcttat
     4321 gaacaaaaag gctgaacaat tggagcttaa tgatactcat tttgtaacgc cgtcagggct
     4381 tgatgatgat acagatgagc attattccac tgcagcggat atggcaaagc ttgcagcgga
     4441 agcaatgaaa aatgaagatt tcagggctat atgtcacctt gaaaaggttg agttgtgcta
     4501 tggaaaccca ccgtatgacc gctggctaat taactcaaac aagcttttaa aaagctgtga
     4561 gggtattgtg ggcgtaaaaa cggggtttac ggacaaggcg aggagatgtc ttgtttcagc
     4621 ctgtgacaga aaggataaag agcttatatg cgttaccttg aatgctcctg atgattggaa
     4681 tgatcactca aagctctatg attattgctt tgattttgta tctaaacaaa agcttccact
     4741 ggataaaagt agttttgatg ttgcagttgt tggcggagtg agcgacagtg tgaaatgtaa
     4801 ggtagggcat gcaagtgctg tgcttttgaa cggcagggct tacaaagtca agacaaagat
     4861 atatctgcca aggtttgttt atgcgcctgt gaaaagtgga gataaaatag ggctggcatt
     4921 gttttactac aatgatgtag aaatagcccg aacggatatc acggcggtgg aagattcgat
     4981 gcttgcagag cctgaaaagc cagatatatt ccactattat ctagataagc tgaaaaaatt
     5041 gtttgcataa cggctcaatg tgagccgtac ataaataagg agaaaatatg gaaaagattc
     5101 gtatacagaa aataatgtct gacagtggtg tttgttcaag aagaaaggca gaggagtata
     5161 tctcagaggg aagggtaaag gtcaatggca gaccttgcaa gcttggcgac aaggctttgg
     5221 caggcaagga tataatcaca cttgatgggg aaaaaatata tgttgccaag aaaagacagc
     5281 tttattatat catgctccac aagccaagag gatatgttac cacaatgagc gatgagcttg
     5341 atagaaagtg cgtaaccgag cttctaacaa aagtgcctga aagagtttat cctattggca
     5401 gacttgacaa gaactcggag ggactgttgc tttttacaaa cgacggaaac tttgccaatg
     5461 atatcatgca cccttcaagg catatcgcta agacttatcg tgttacagta agacctgaca
     5521 taaacgatga gcagctcata aagcttgctt caggagttga aatagacggc aaaatgacgc
     5581 aggagtgttc agttgtcgtg ctcgacaaac agcctggcag ggttgtgctt caaatgacta
     5641 tccatgaggg cagaaaccgt cagataagaa aaatgtgcga agctgtggga cttgaagtcg
     5701 caagactaaa gcgcactgcc atcggtccga ttaaactggg catgctaaaa ccaggtgagt
     5761 atagagagct taaacctga
//
LOCUS       DAWEMR010000164         5742 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5431_length_5742_cov_10.260243, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000164 DAWEMR010000000
VERSION     DAWEMR010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5431_length_5742_cov_10.260243"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1688 a         1277 c         1149 g         1628 t
ORIGIN      
        1 tgccaaaggt ttctagatat gcaaatgctg tatgaagcgc accaaatccg cccattgaaa
       61 gacccataat gtatgtttca ctgccatctt tcgcaagtct gaaaactcgt cttacatagt
      121 ccacaagctc ctcgccgacg aatttgcaga actgatgacc agtcgagcgt ccgtccagcc
      181 aaaaagaatt ttcgcccgag gggataacta ctgcaaaatt atacttgtcc gcaagatatt
      241 caggcaccca gttttctgcg tcgcctgtaa aaccgtgcag caagaaaagc gttttggtgc
      301 tgttttccga gtatttcgta tctgtctgcc cccagtcctg ccttatatca catggcaggt
      361 acattttaaa cgtagtatat ctccgcaggc aatttgaata aaatctgatc gttaagtttg
      421 acatagcttt tcctcctgtt tttttatata attatactac ataaaatcag gtttgtccaa
      481 gggggcagcg aaaaaaattt tcaatttccc tcacaaaatc atttatccct ataaaaataa
      541 aaatcacatc tgtcaaagcc tcgtcccaac gtaccttgcc gctttttcat aatatttttt
      601 gtcgatcata attctacgct tctccacatt tacaaggtgt ctgtctgccc cgtctgcaac
      661 tattttattc attatatcaa aatcaaagcc gcaaagtcaa accagcaaaa aaagctacct
      721 gaccttgcgg cttttgacct ttataataca atactaaccg aaatgtgccc tacatatcct
      781 ccctcatcat atcattctca ctctttataa acataagcac aaagcatata agcagcaatg
      841 cacaagatat ccatataaac tcattgccta tgtataatga atatgctccg cctattcccg
      901 caccgcaggc aaatacgaat atcgtaagaa aatattgcag cgatttccta agcagcgact
      961 tgtcccctat cctcatgtat gcacttatgg attccattgc acttctcata tttcctatgc
     1021 acatcgtgct tgcgtatgga taactattta ctttcctgaa cgattgcacc tgcattgcac
     1081 aggcacatga tgaaagtgca ttcgcaacaa aatcgtatga accgccaagc ggtataaacc
     1141 ccacaatgca aagcagcacc atttccgtga aaactattat ctgccgccaa tgtataaatc
     1201 ccacgtcctt gaaacgtgcg tgcagacgct cgactatgaa aatccccatt gcaaacgcca
     1261 gcacaggtat aagatagtgc aacgccctgt gccagttgcc ttttgcgaca tatgtgctca
     1321 taagcacaat gtttcccgtc tgtgcatttg aaaaaacttc acctcgcaca aaatacgaat
     1381 atgcgtcctg caagccccct gatatggtca gaagtatcat gagcatattg gtttcagaca
     1441 tttgttttct tgatcgtaac atcattttat catattcctt cataaaaaat taacaagaaa
     1501 aatattttta tacactttaa ttttctcctt tttccattat aattatctca gatatataag
     1561 tcaaatatat aatttatatg caagatataa aatattgata tagcaagaaa gtgataaagt
     1621 gaatataaat tacgaatatt ataagatatt ttattatgcc gcaaagaacc gcagcttttc
     1681 aagaacggca aaagtgctgc tcacaaatca gcctaatatc acaagggtga taaaaatact
     1741 cgaaggagaa cttggctgca cgcttttcaa tcgaatgaaa aacggcgtca cgcttacccc
     1801 tgagggagag atactttacg agcacatctc aaaggcggtg gaacttatta cagagggcga
     1861 agatgagatc tcaaaagcca acgacctgca aagcggaact gtcaccataa gtgtgacgga
     1921 gaccgcactt cacggcgtta tgctgccact tctcaatgag ttccatgaaa gatatccggg
     1981 aataaaaata aagctgctga accattcaac ccctcaggca ctgactgctc tcaaaagtgg
     2041 cattgcaaac ctcgctgtgg taacaacgcc gataagcaca gagggctcgc tgaattttca
     2101 taagctcaga gagttccgtg aacttatcat atgcagcaca aagctaaagg atcttgcaaa
     2161 gcgaacgctc actcttgagg aactttgcag ccaccctata atatcactta accgaaacac
     2221 aaggacatat gatttctatt ccgatttctt tttccagcat ggaatagatt ttcagccgga
     2281 tatagaggtc gcaactgccg atcagataat gccgcttgtg aaaaacaacc ttggcatagg
     2341 tcttatacct gaaccaatgg cacaaagcgg cgatgtttat attctgaaaa ctgatgaaga
     2401 gtttccaaag aggagcatcg ttcttgcaga aaatgcagac aaaaaactca atgccgccgc
     2461 tacagagata caaaaaatga tactgaaaaa atgtaatatg tttgcaaatg ctgagatatg
     2521 acatacatga aaggagataa taatatgcct acagttttgc caaaagaaaa tgaagtcgaa
     2581 cagttttttt caaggataat ggcatcggaa ggagcttgcc aaaggctcag caacgtgctt
     2641 tacgatcacc ttagcgacga cgatatgcag accgatcctg acccagaaca tcttgtgcag
     2701 gtgcttttga ccgcatatac gaacggtgat ataagtgcac ttctgctgga gctttgtgga
     2761 agaagtcttt tcgatctgct gagagaggca taccttatac caaagcgttt tcacggaaaa
     2821 gccggaaaaa atcctgaact ccttacgacc cctgaaggcg atgtcattga agaaaagaaa
     2881 aagctagtca caaagcatga gttcgagaaa tttcgtgatg tgtacaaaaa ccacagctgt
     2941 gcaccacgtt caaagcttta tcttgcagac ggatatgacc ttatgagggt ctataccgac
     3001 ggaatgaaca ttaaggaaaa gcgtgttgac aaaagacgtg gcgtgcttat actttacgcc
     3061 ctgcctgaca ctgtaaagct tggactttcc gaggctcaga cctatgatgt tatatggaat
     3121 actttccaca agatacagca gactgcccca accgcaatgg tctattacgg acaagaaaca
     3181 ggcgaaaagg ctggaaaatc atttgatgag atcggcgttt tactgcctgt tcatcagttt
     3241 gaaaagaaaa tgctcatgca cctcagcgag atagacggca ttgtgcttgc ctgccgtgag
     3301 gaaatgatga aaaaagcagg ctcagacagc cttgatcttt aaaaacggaa gtgatactga
     3361 atgtcacaaa ctatagttaa gaaaaatcac atggatatcc catggcacga cttcacagac
     3421 gccgaaaagg acgagccagt atcaaaggcg agcctaaaag aaaaagcctc tgttataggc
     3481 agggtgggaa tgatgtttct ctcctgcgga acgggagcgt ggcgtgtgag aagttccatg
     3541 aacaccatgt cggaatgtat gggactgacc tgcacagcag atatcgggct tatgtccata
     3601 aactacacct gttttgacgg cgaggcttgc ttttcgcagt ctctttgcct tactaacacg
     3661 ggagtaaaca attcaaagct aacacgtctt gaaaatttca tcaacgattt tgtcggccgc
     3721 tgcgatagtt tgacttgcga acaggtccac aatgaacttg acgatatcga aaagatacac
     3781 ggacttgact cgccgcctgt tttgggcctt gcggcggcac ttgcctgcgg agcgttcaca
     3841 ttcctgcttg gcggtggact gaccgagatg atatgtgcat ttttcggtgc aggcattggc
     3901 aattatgtgc ggagcaaatt cacaaagcac caccttaccc ttgtgcttgg aatagttgcc
     3961 tcagtatcgg cggcgtgcct ttcatatgca gggctgtttg aacttgcgaa aatacttttc
     4021 aatataaaaa tgcagcatga ggcaggctac atatgtgcaa tgctttttat tatacctggt
     4081 tttccgttca tcacaagcgg catagacctt tcaaagctcg atatgcgttc gggcatcgag
     4141 cggctgacct acgccctcat cattataatt gtagccacaa tgacggcgtg gttaatggca
     4201 cttatcctgc accttacgcc aatggacttt ttgcctctgc accttacctt atggcagttc
     4261 atactgttca gacttgcggc aagtttctgt ggggttttcg gattttccgt tatgttcaac
     4321 agccctgtca agcttgcagc ctctgcggca gttataggtg cacttgcaaa cactctgaga
     4381 ttggaacttg ttgaccttgt ttcatttcct ccggcagctg ctgcgtttgt gggggcattc
     4441 accgcaggca tactcgcctc gctgctgaaa aagtatgtgg gctatccgag aatatccata
     4501 accgttccgt ccatagtcat aatggttccg ggattatatc tttacaaggc tttctataat
     4561 ttgggcgtca tgtcccttga aacatctgcg tcatggcttg cgtctgctct gctgataata
     4621 ctagctctgc ctttggggct tatcttcgct aggataatta cggacaggtc atttaggtgt
     4681 tgtacttaaa atcttagtca tagctaaagc tatactgaac ccacataaaa aaactatatt
     4741 gatatacact ttcctattaa gactattctc cattacctat aactggcgtc tgcgcaatgc
     4801 ggcatatatg ataaaaaaca catctgactc gataaatgct atctgtgggc tttgcggctt
     4861 tgacgatcac agctattttt caaaagcctt taaagaagcc tacgggtgca gccccagaga
     4921 ttttagaaag tagaatatac gggacaatat ctcacagatt tatccgtctg tcgtgtagac
     4981 attacaattt cctgtttata ggactacgat aaaactatta tcaaacaaaa gacgtttcat
     5041 tttatccaaa tggaacgtct tttgcatttg tgcttattct tgtatgaaaa agagtcaatt
     5101 attctcctcc caataatcct ctgcatcctc gtagtcatag aaatcatcat agtgatcctc
     5161 atagagatcc tctggatcgt cataatcatg agcatcatac tcagcaaatt tactggttga
     5221 ggttttattg gtattcttac gggtggctct ggtagctgaa ctacttgctg tagtggtttt
     5281 cggacgtgat gttgtagcag ttgtgaatac tggagtatag ctgttcttat tattgtaata
     5341 cttagggtca tgtatcaaac agtagcaact gccttcttca ggagtattgt cacagcctga
     5401 gtgtgcgcat ttggggcgca gggcatcata gatcatggct atccctatga tgatcaacac
     5461 agcgacaacg attcctttga gattgaacga actatgctga cagctgtttt ctgttgtcag
     5521 cttgtagact atataatcgt caagcatttc ctcgtttctg ttgttttttc cagattccat
     5581 aatcacacct ccaaactgta tttttaatat tataccatat tttttaagat ttgcggtaac
     5641 aatttttgga cagttgaata ttctgcccat tttatcattt ttttagtggc aagtcgggag
     5701 atgttgccca cttggacgtt tttataaagc aacgaaccat tg
//
LOCUS       DAWEMR010000165         5676 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5506_length_5676_cov_11.005337, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000165 DAWEMR010000000
VERSION     DAWEMR010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5506_length_5676_cov_11.005337"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1269 a         1474 c         1299 g         1634 t
ORIGIN      
        1 ataatcagtc ttgcggtctt cgtttttttc ttagagaatc ctgcctgctc caatgcggta
       61 taaaaatcag ttgtacttct gacaaattcc ccattctgcg cacaatagtt acggtactct
      121 tggtagagat caccggactt ttcagtgtag gacttatcga cctcacagca ttcagcaata
      181 aaatttccaa gccagtcgtt gtcatcacgg tacgagccga tcgccgcctt aacgcaatcg
      241 ggagctttga tctggtaatt tgccgaaaca accttttttg caccttcgat cagccacttc
      301 aaaactgcac ctccagcgtt gtcaatgagg tactgcgtgt aattcttgat atccgctgct
      361 ccctgaatct ttgcgtggaa aggaatcacg atcagtcgtc tccaagtacc gtcatcagag
      421 gctcccacct ttggcaggtg attcgtatag aggatcagcg tgtgggaagg ctcgaaatcg
      481 aacggggctt tgaacttttt ctcggcaaaa atgctgtcag tggaacagag ctgttttacc
      541 acggaggtgt tcagtctcat gccttcctgt aactcggcag cgataatcag tcgtttaccc
      601 ttgagctctg ccatttcggg cttgacattg cgcttgcagt tcatggtcaa agcatcagcc
      661 gacatattgc cgctgtagct tccgaggact ttgtagatga cattccaaaa agtcgatttg
      721 ccgttgcgtc cgtcaccata agcgataata agcgattcaa cgtatacttt tcccacgata
      781 cacagaccgc aaatcatctg cacatagtcg ataagctgtc ggtcaccgca gaagaataat
      841 tcaagtgcat cgagccacaa ctgcataccc tcgtccgagg gaacaacagc ggtcactttg
      901 gtcagcaggt cttcagggtt ggtagctctc caaccgtcca tgccatcggg gaggtagtaa
      961 gtgccgccgg gcgtgttcag gagcattgct tctttgtcca gtgcttccgg cggcttctgc
     1021 accagagctt ttgccgcatc aagggcgttg ttcagaccac gcatattgcg gtatttcatg
     1081 acgaatgcct taaacgactt tgcaaagctg tatgcctcat aagcatcaag ctgtccatcg
     1141 aagagagact caacgaattt ctttccgccc ttgagagcct gttccatgtt gatgcctgcg
     1201 ttctgcattc gttccagagc tgtcttttca agctcatcag ctttgttgag ctgtgcatcg
     1261 gtgtgctcca tcatcgcaag caccgccgac tgctcgtttt cttcccaata agtgccgttg
     1321 tatctgagat aatcagtcgc cttggtgaaa acaagctcat caccgaaaca gtccgcaagg
     1381 gtacgagctt caccgacatc ggagaagtca tcggggatca tgctgttctt gccatattct
     1441 tcggggctga catagccttc ctgctttgcg acctttttgc cgaacttaca ggcagaatgc
     1501 catatcgttt cgagttcctc gtcatcaagc ggagggtcac actcggcagc catttcgaga
     1561 aacttctcgt gcgcatcttc gccgataccg aaccgtttca cgatacgacc tgcctttcgg
     1621 gacatggtcg cattacgctg tccctgcgga atactgcggt tggacttcat gaaaagaagc
     1681 cagtcctcga tggagagcga tccctcatgc cactcgactt ctcccgtact gccgaagaag
     1741 aagcgagctg cgtcaagagc gttgctgtcg aagaacggca tttctttctg aatgcggcgt
     1801 ttcatcttct tgtgcatggt ggcatccttg caaatgccgg cagggagata gacgtgcatc
     1861 ttggggcgag cggtcttgcc gtttttcggt atcatgtgac tgcggctgta tacgatagcg
     1921 tatgcaacat cggtgaggat cgccgacagc ctctcggaat cgatccattc ttcggggtca
     1981 tcggaatggg tgttgtcgca gtcgagtaca agcacatcgg acatctcgaa attcgcctca
     2041 ctacggacat ttccagcata tctggcacat acatggtcga agcgtacagc tttctttagg
     2101 tcagccgccg aggtgacaat catctcgtgg ggataagcct tgttcttcgg attaccactg
     2161 caatcagcgg tgtagagcgt aaatttcatt cgttttcctc cttctcaaaa ataccgttgc
     2221 atttcgggca gtgcgtgaag ttcttttcct tgccgagttc atgattacta cagacaggac
     2281 atacagcttt tgtatcgagg tgatgttctt ccttacgggg cttgcggcat tcctttttgc
     2341 gaagctcggc aatggcagcg tcaagcacct tgatgtcgtg gtagaatacg tcattgaagt
     2401 cgtcatcggg tccgatatgc gactccgtat ctttgcggag cgacatcagc cttgcaataa
     2461 tttcgctgat cctcattttt tcttctccat ttctgccgta aagtagcgga tcttcatgta
     2521 tttgcgtttt gcctgtttga tctcctcgct catgcccttg gagatcacat cgccgaacac
     2581 ccacagttca cggcagtgac tgaggagaat attgttcatc agcaccgcct cggctctctc
     2641 gtcctcatcg ttcataaact gcgtaaagta gatgtgaggt gtgatcggca ggcaatggtt
     2701 gtccaccgca aaacggctgt atttctttgc gttctcggtg ttctgctcga tgttccctgc
     2761 gtatggcgag cagatgtata cgataggacg aaagtttgcg gcttttcggg cagccttttc
     2821 ttccttttcg atgcgagtca tcgcttcata ttcggtaggg ctgaagtagc cctcggagtt
     2881 gtagagattt gccacgattt atacctcctt tgccagcatc tcacggagaa tagcggtgaa
     2941 gatctcatgg agctttttgt cgccgttgat gatgtcgagg acagacaccg ccttaatagt
     3001 cgtcttggtt gcaccggtct ccaacaggcg atttttctta ttgcgcaccc tcgctgtcag
     3061 gcatacacct gcacggtact ccaatgcgtc ataggctctc tgatagacgg actcaatgcc
     3121 ggagtagtcg ccgcattctg ccatagcaat cttgccgacc atactgcgga catcatcacg
     3181 ccagtcatcg aactcgaccg taaatgcttc ctgaatggca ttgaccttgt caccgaccgc
     3241 tgtaatttca gcctgctgtg cattgagctt ctgttctgcc tcgatcatcc agcggagtgt
     3301 cggggaaagg ctcatatcgg caaggtaacc gccggtcttg cggatcgcag gcagtacctc
     3361 cgaggtcacc caatgtttga agcgttttgc tgtggggagc ttgctgcaaa gaacgaggga
     3421 gtagagaccg ctttcgttga taacggtcag cttctgtgtt ccgccaaggg tgtcgcattt
     3481 tgcgacatcc ttatcctcat cgtcaacgtg cttgaaaatt gcatctctgg cattgctgta
     3541 tccgagagcc tttgctacat ctgctcctac gaaccacggc tcaccgttaa tgtccattgt
     3601 tctgatttca ccgaattcgg catttttgta aatttcgtat ttcatgtaaa tatcctccaa
     3661 ttctatctgt ccgtattggt gcggacagtc cttttcgtta gttatactgt ctgtattggt
     3721 tgtttgtgcg tctttaccag agctatttaa cgtcttttaa agcgaagtgc agctcacacc
     3781 actttatcgc tgtaagtgta tgctcacggc tgtctgtaaa ccagccattc gtcaccataa
     3841 tcgtgctgat ctttcttgaa atgcagtaaa gattctctat gctgaaatct gtcttgtccg
     3901 aattcaaaaa gacgatcaca tgacctttcg gcactttctt gccgtggtgc tgttcccaaa
     3961 taagccgctg tttttcgata taatcctgtg tcttaattcc tgtgtctttg attttcacat
     4021 aggtgtatcc gttggaatgg atcgactcac tgcctacagg agcattattc catgtctgat
     4081 gtcccttttt atatgcctgt ctaccggata ttagtttgag aaatttagca caatgtgcct
     4141 gaacacctct ttcagtcttt ttcgcaccaa atcgcttatt gaaagcatcg gctactgcaa
     4201 cataattagc gtactcatgg caatgctctc tgagccattc atcctgctcc tcggtaaaag
     4261 gatgatgaac acccgctata ccaaggcttc tgcatttatt tttcagcgat gatgctgtaa
     4321 cgaagagtcc aaaataatct gcaaactgta tcgcaagttc tgcataagtt gcagttccga
     4381 ctttatcacg aagccactgc atttcctgct ccgtatagtg gtgcatctgc ctcatcggat
     4441 cagcttctcg atattggagt tctccagctt tccttcagcg accagctttt cggtgcggag
     4501 aacgacgtca gcgttgttga tcatctgctt ggcgagcgag gagatcgtct gagaaagctc
     4561 agcctccttc ttgcgctcat ccatactcat ttcagtattg cttacgatgc ggattctctc
     4621 accgagaatt ttctgtaatt ccataagtgt cataaaactt cctcctcaat ctttcatata
     4681 aaattcgcat tcatatccgt ctgcacggag cgtcagtcct cttgcccatc ggggagtgat
     4741 acacattttc tgcacgatct catcaacggt tatatcgtca gtagcttcga taatgacttc
     4801 atcgtgtaca tgagcgacaa tgcgataatc cctgagattc atcattgcat ggagcagaat
     4861 atctcgtgca atgccctgaa tgatgttctc cacgaacttc ggtccgtagc tttcgatacg
     4921 ttcccacttt ttctgctcac caacacccat gtaagtgacc gattcgccgc cgaacttgtt
     4981 ttcgccgatt ttcggtttca cataggcaag tcgtctgccg gatggaagct cgatgaacag
     5041 aaatcctgac tcacagaaga tatgcagtcc cttgacatta gcctctgtct tttccttgat
     5101 gcactttttt acggcacggt caacatccca ccagagcttt acgatatttg gagatgcatc
     5161 cctccagtcc gtcacgatct gctggatctc atcatcagac agtcccattt cctgtgcgcc
     5221 cattgctttc atcgcaccta cggagccgcc atagccacag gcgagctcgg ctattttacc
     5281 cttctgtcgc agatgcccat taataccgtg tttcacaacg ggaactccga atatctttga
     5341 tgcagagata cagtagatgt cgccgccatt tttgaacaca tccaggcgcc actgttcacc
     5401 tgcaagccac gacagcacaa caccctcaat gctcgtgaag tccgctacaa tgaagcgttt
     5461 gtatggggct ttcggtacaa gagccgtgcg gataagctgt gacagtacat cgggaatatc
     5521 tccgaagagg aggtcgagcg tttcgatatc atcggctttg atgaggtctc ttgctgtatc
     5581 gaggtgaggc atcgaattac gataaaggtt ctggagctgt accagcctcc cgctgaatct
     5641 ccctgaacga ttcgcaccgt aaaactgaaa cattcc
//
LOCUS       DAWEMR010000166         5646 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5541_length_5646_cov_9.038812, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000166 DAWEMR010000000
VERSION     DAWEMR010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5541_length_5646_cov_9.038812"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4317..4326
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2106 a          811 c         1161 g         1558 t
ORIGIN      
        1 gacattacat ataagtggat tacttatgct aaaaagtttg gtgggtggat tgattatgat
       61 gagtgtaaga aactgagaaa cgcagaaaag aaacgcaaga aaacggagga aaattgaatg
      121 aaaaataagc ttagttttgc ggaaatgcag gcatttataa ataatgtaat aaagggtaca
      181 attgagtacg gtgcaggata taaagttgtt ttgcgtaaat attatgtgct tactctttac
      241 ggagaatata aattctcatc agacgatatt gcggagattt atgacagtgg cgagttggat
      301 agagaatatg ataacattga tcggaaattg attgatggcg atcagtatta cctaattact
      361 aaggctattg acagcggtat tgacatgaac gttagataca aggcggctga aaaagttatg
      421 agcatggcaa atatagctat aacggagctt gcaaacaggg caaaagaaat gatagaacag
      481 acaaataata ctatgaagga ttttgacact gaaagcctaa atgagatact gaaaacattg
      541 aaagacagta atgacatggc aaataaaatt gtaatttcaa acaataagga cggtgactaa
      601 tatgttcttt gcagaacagg aaataacact tggaatagtt cctaatgcta ggaatattca
      661 taggtttgtg tattttacac aggtacgccc ctctgtggtt aatctgacaa cagatagaac
      721 ggtcaatggt aaatcaatta taggtctttg tagccttggt ttaagaaatg gtgacaaagt
      781 tacgatagaa acacatagta aagtttctca gaagcaagct gacgaggatt taaagcttgt
      841 tgtaaattgg ttgcgtggtg aggaataaat ggttgtaaaa aaccttaaag aactagagcg
      901 agaactaaga acaagaattg attacgctct gcttacagat gttaccgagg ttgttaccac
      961 tgttatgcta gatcatattg aaagagatgt ttacgatagt tatgtaccac atgaatatgt
     1021 aagacgatat gataatggtg gcttaatgga tattaacaat attaattctt ctattgaagg
     1081 tgacacttta gttgtagaga ataatggcta atccatatat ttttgttcag agcaaaatgg
     1141 ttaagtcaga taatgcagac caagaattag cacctatcat tgaaactggt tgggggtacg
     1201 attttggaaa ctggacgtat catggtgttg ctagaccatt tgtatataat acaaaagagg
     1261 atttaagtga taacaaatat cacattatag ctttaaggca aggacttaaa agacaaggaa
     1321 tagaggtgaa gtgaaatggc agatgattta aaaatacgag ttcctgtgga acttgacaca
     1381 agtaaagtta aggacgatat acctaaatta aataatatac ttgcaaatga caataaggct
     1441 catgctaaaa tcattggtga gttggatttg aataaaacac aaaagaaaat tcaatctcaa
     1501 cttgctacaa tcagcaaaaa tctaaaaata gatattggtg gtttaaatgt aacttctatt
     1561 cagagtggta taaaggttgc tgaaaaacag gtagttagct ctgttaaaaa tataaagcat
     1621 gagatacaga atattgacac aactcttgca gaaactttca aggcaggttt taataaagac
     1681 ggacagatag atattgttaa aactattgaa aatgcaagaa aagttttgag tcagtttggt
     1741 aatccgacat tttcatggac taaagatagt tcgggtgaag ttactcaaat tacggcagaa
     1801 gttacaagct tgacaggtca agttgaaaaa ctgaaatatg ctctgaacga aacaaatggg
     1861 tcatttgact atctatcggg tagcagttct gaaaagggta tattaaagct gattgcggat
     1921 attgataagg ctaagtcaaa atacacaaca cttctttccg agtttaagtc atcaaattcg
     1981 ggcattgaaa caggactcac taaggaaatc acagatgtta ataatgctat taataacctt
     2041 ggtaaaggtg gctctgttgc ggaagttgat agtttattta atactctcaa gactactgcg
     2101 aacgagatta agcaaaatct tgataccact tcaagttcat ttaataaagt aacaaatgct
     2161 gaaaacactt tggcaaaaat gcctgccaca atacaagaaa tttcaaataa cttttctaag
     2221 ctgaaaaatc agccacaaga aattgtggat ttaattcaag gtttaaacac tcaattaatc
     2281 aaggtaaaag ataccgagga gaattttgga cgcaataaac aatggtctga agaatatcgt
     2341 gagttagttg tttcggttaa aaaagcagaa acagaaataa agagcttaca gttacttgaa
     2401 aaatctgata attctgaggc acagcagcaa gctcattatt ataataagat gtttggtgaa
     2461 atcaaacaga ttaataagct taaaaagcaa caggtcaatg ctggcgagca agaaaatgtt
     2521 gagctaaaaa gacagattaa aaatcttgag agtagagttt cttatgacga gaagcagctt
     2581 aaaaagaaga aactgattac agaagaactt gaaagacaaa aaaatgaatt aataaacatt
     2641 ggtagggaag aacttagatt agccaattct cgttctgctg ataaatcgtc agctacatct
     2701 actaaaacag aaaataatgt agctagactt acgcaaaatc tcactacctt agaagcaaag
     2761 tggaaagagt cgcctatctt taatggagag ttccaagaaa agtttaatga gttaaaaaca
     2821 agtttgtcta atgtaggtgg cgatcctaaa gcattagacg aatatcgtat taaactcaat
     2881 gaactaacaa atgagttaaa gagggcagat gtagcttata aagctagttt ttctagcaat
     2941 aaatcacaac agaatataga agctacaagg cagaacatta aaaagttaat atacacaatt
     3001 cagacatggc aacaggctaa tactaaagcc atgggcaaga atacttttaa tggcggtaca
     3061 tatcaggttg aaactgataa tatgatagcc tcactcaaaa agttgcttaa tgctagcgat
     3121 ctaactgcga gcgatttgaa agtcaatgtt gataaaatca atcgtagttt taggacaatg
     3181 agttctgaag cacaggcagc aggtgtgaat gggttaagct ttttcgataa gattaaagag
     3241 gacgctttaa aattcacaag ctggatgagt ttaactactg tgatttcagg tatatcaaga
     3301 gaagctgtta agttctataa taatgttgta gatattgata cagctatgac agaattgcgt
     3361 aaggttactg ataacacaaa tcagcaatat gccgagttct ttgataatat aggtcaaaag
     3421 gctaaagatt taaagattga tttgtctgat cttatttctc aaaccgcgga atggggtaaa
     3481 cgtggttata gtttagatga agctgaaaca cttgccacaa actcaggtat ttattcagtt
     3541 gttggtgaag tagataatgc aacagcagta caagacctaa caacagttat gaaaagctat
     3601 aacatgacag ttgatgagtc tatcaatatt gttgataagt ttaacgcaat atcaaacaag
     3661 tatgctgttt cagcaagtga tattggtgat atgctatcaa ggtcagtatc ttcactgagc
     3721 gtagcaggaa atacactaga ccaagcaata gcaatgggta cagccattac agaaataact
     3781 ggagacgcag ccgaagcggg taaacgcaaa ttgcccgact atataataat atatagtatg
     3841 cagataacta tatcggtcaa aggctaaagg atagttaaga ccgaggtaag actcaatttt
     3901 ttttgagtaa ccgtagagac tacaggatat atatggcaac atatgtattg aagttatccg
     3961 tccttattac agggcgtaat atatagtccg agcatcgtat tataatccta taaaaaaaga
     4021 aatacgagag ttagccagaa atgactaacc gctacatatt taatgtagtc agtaccaata
     4081 taattgggaa agtaacagat tgaacagttt gaaagttctg tcaatgcgac ttcgtggagc
     4141 gaaaacagaa ctagaagatg caggcgagtc aacagagggc atggcagtat caacctcaaa
     4201 actgagggaa gatattaaag ctcttactaa tgtaaatggc acaggtggct ttgacataat
     4261 gaaggactct cagaacttta agagtaccta tgaaattatg aaaggtatcg ccaatgnnnn
     4321 nnnnnnatgt ttggaacgac cttactgata catcaaaagc cgctgtcata gagaaaattg
     4381 caggtagagt ttacctgaat gtacagaaat gtgcataaag aatatattta attgcaggta
     4441 atgagtaaag ccttacacca caataatgaa gaaattacat tatgacggtg cgaaagcaga
     4501 aataatgtaa ggattgtata aggtcaaaag cctaagtaca gtaacaatct ctgttcatgc
     4561 agctaagtac cctaacgtta tcctagatca taggacagtt taagtcgagg gtaaaagttc
     4621 aacgactatt ccccatatgg ggttgtaaca ataaaataaa ggtggaaatc ctgaatagtt
     4681 gcaacaaaag aagtacggct caatcgcaaa tggagtggga gaataaccct taaacggaaa
     4741 aggtataatt gctgtcataa atgacgtgat taagaaatag tctaagctct atgtgaaagc
     4801 ataggatatg ttatataaca tataagtaaa tttgcgactt actttaatat aattgaagca
     4861 aagaggcaat acaattactg cattgcttac gaatatgagt caagcggata aaattgttaa
     4921 tgactcaata ggctctgctg ggtctgctat gtcagagtat gaaaaatacc ttgactctat
     4981 tcaaggaaga gtgcaaggtt ttcagacaag tattgaaaat ttgtcagcta ctctgattaa
     5041 tggtgattta gttaaattcg gtatcaccag tggaacacaa attattgatg ttcttgataa
     5101 tctaattagt aaattcggtg ttttagaaac acttattcct accgttatgg caggattatc
     5161 attcaaaaac gtaggtaaac aattattaaa gatgccaact tatgcacagc cacaaactat
     5221 atgtgcatag gtcacacacg ttttaaaata aggttgccaa attgctggga acggctaaag
     5281 ctttgcaact acttgtagca atggtattac aagagtgagg aaactcggaa acaatagcaa
     5341 agataacata tgctgagata aaagcctatt atactattac aataggtgct aagtgttgtt
     5401 aaaaatgtca ggtcagcagc caacccctat cgggagatac ggactaggtt cagagagtag
     5461 acggtaacta tcttgtggta agataaaggt gtactccaac tataggtaac acctatagcg
     5521 tttcaaaaaa tgaattatcc ctcatttatt tagttttgtc ctttgacagt gagggtggga
     5581 taaaactgtt accaatcatt ttgcatagtg atttatttta cactattcat ttgcgtatgt
     5641 caacac
//
LOCUS       DAWEMR010000167         5621 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5558_length_5621_cov_13.721524, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000167 DAWEMR010000000
VERSION     DAWEMR010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5558_length_5621_cov_13.721524"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1515 a         1516 c          983 g         1607 t
ORIGIN      
        1 tgatatactc cgccttttct cttgaatacg ctgtaagttc tgccttcata gcatcaatat
       61 cagcctgtgt gattttgtca ctcttcttag ttgtcgccgt cacgaccccc gaatacttgc
      121 catagtgatt attattcgtc tttttgtcgt cctgcttgca agcccttatc ttgaactgat
      181 actttgtcgc aggcgcaagc ttcttcacag tgtagcttgt ccctgtggtc ttgcctatct
      241 ctttccattc gccttttatt ttctgataaa tgcggtaatt tgtacagcct atcttcgtcc
      301 aagaaagctt aacgctgtca gtgccaacac tgcttacctt tagccccttg caagcctgcg
      361 gattggtcgc cgtgttccag tttgcggaat attctccgta aactttcttg ccctttacag
      421 tcttgtatgc ccttacttta aaattttctc tcgttgcatt aggcagcttc ttaactgtgt
      481 agctcgtacc cttgcaatcc ccaagcttgt tgtacttgcc gttttttcgc atataaacag
      541 tgtaaccgct cgcacccttt accttgctcc atttgagctg tatctcactg tttgaaagcg
      601 tcttagattt aagccccgtt accttgccaa cactcgccgc ttccgcaacg atagccgtct
      661 gtgagaatat ccccatatcc tccgccgcag gtacaacagc ccccgtgccc ataagcattg
      721 ccgccataac aacagccgcc gttttcttca taccattctt cataaaaagc acctctcccg
      781 attctctata attccccata tgtcatggta atattatata ataccataaa gccaaatatt
      841 tgtcaatact tttacaaaaa aatcatcaaa tattttgcaa aatttcgtag gggcgaacag
      901 cgttcgccca tcgaagcaat acacaaaaag cacaacgccc acaaagagca ttgtgctttt
      961 atcatatcta aatcaatcag ctattcttct tgctgtcatt ctctcttatc tcaacacggc
     1021 gtatcttacc gctgatagtc ttaggcagct cgtccacgaa ctcaactatt ctaggatact
     1081 tgtaaggtgc agttgactgc ttaacatagt tctgaagctc cttagcaagc tcaggagaag
     1141 cagtgtagcc ctttgcaagc acgatagtag ccttaactac cttgcctctg atagcgtcgt
     1201 cagccgccgt gatagcgcac tcaagtacag aagggtgttc cataaggata ctctctatct
     1261 cgaaaggtcc tatacgataa cccgaagcct tgataaggtc gtccgttctt ccaacatacc
     1321 agtagtagcc gtcatcgtcc atgtaagccg tgtcgcctgt gtgatacata ccaaatctcc
     1381 aagcgttgtc cgttgcctca gggtttctgt aatactcctt gaacagcgca gggttttcgc
     1441 ccctcttagc gtaaacaacg atctcacctg tctcgcctac cttaacaggc ttgccctcgt
     1501 tgtcaacgat accgatattg taaagcggtg taggcttacc catcgagccc ggctttggct
     1561 ccatgccgta aagattgccc acaagcacag cgccctctgt ctgaccgaag ccctccataa
     1621 gcttaagacc tgtgtactca taccacttgt tgaatacctc agggttaaga gcctcaccgg
     1681 cgatacagct atatttcagt gaagaaaggt catagttttc aagaccctcc ttgatgaaaa
     1741 atctgtacat tgtcggaggt gcgcagaaag aagtcagctt gtaatcctga attactttca
     1801 gcacgtttgc aggaataaat ctgtcgaagt catatacaaa agttgttgca ccgcagataa
     1861 actgaccaaa catcttgccc catgcacact ttgcccaacc tgtctcagaa acagtaaggt
     1921 gcagtgagtt ctcatcaagg tggtgccagt gcttagcagt gataatgtgt gccgcagcca
     1981 gtgaatactt gtgcagtacc attttaggat aaccggtagt accggagctg aaatacatga
     2041 gcattgactc agtaacgtgt gtttcgatcc tctcaaaagt ctttggatac ttgtccattt
     2101 cgctgagaaa atctatccaa ccctctttag caccgtgagt gataaacttc tctttcatgc
     2161 cgctgtactg ttcaagagct gaatccacct gattaggaac atcgtcctcc gcagttgcca
     2221 cgatataaga gatagaagca gcgtcaaagc ggtaaacata gtcttttttc ttgagctgac
     2281 aagttgcagg cacaaggata gcgcctatct tcataagacc gtaagccaag aaccagaact
     2341 cataatgacg tttaagaaca gccataacaa aatcgccctt cttaactccg tgtgccagca
     2401 tcatgttagc cacctgactg ctcttttcag aaagatcctt gtaagtgaag atatgctcct
     2461 caccctcctc gttgacccac tgcatagcca ccttatcagg cacgttctta gccagctcgt
     2521 ctataacgtc atatgagaag ttgaagttct caggcaggtc aagcttaaag cttttaagtc
     2581 tgccctcgct gtcaagctcc tcgtcaacaa atctcttgta aaaatcagcc attacaaaac
     2641 tcatatatga aatcctccgt tttaattata tcctgtttat ctcatgacaa atgctcacta
     2701 tgtcctgcgg aacttccgca atatacttcg cacctgcatt ttcaagttct tctctgcccc
     2761 tgaagcccca ttcacagcca atgcagtcaa ggcccgcatt ttttgccgtc ataacgtcca
     2821 catcagaatc tccgataagc acagcttcct cagccttgac cccaagcgtg ccaagaatat
     2881 tgtatattat ctcaggcgca ggctttgtcg gaacaccgtc ctttttgccc tcgatcaggt
     2941 caaaatcttt ctcaccgaaa atattcttta caatgtagtt cacaaagccg tcaggcttgt
     3001 ttgaagccac cgccgtcata atgccctcat tgcgaagcgt gtcaagcacc tcgcagatat
     3061 gctcataagg cttggtcttg ttcacacatc tcttgttgta ttcctcagag aactcagcat
     3121 gatacttttt gatgttctcc tcgcttctca gcttctcagg caaagcccgc tcaacaagct
     3181 tcaaagtacc atttcccact aaatgcttgt aggtatcata atcaaacaca ggaaacccat
     3241 atctctcaag tactgcgttc atggcgtccc caagatcata aatagaattt acaagcgttc
     3301 cgtccagatc aaaaataaat agctttttca taaaatcagc tatacaaaaa gcctttcctc
     3361 cctccgactt tccataaact tgtcggtcac catgcactaa tttttacact tatcgcaagt
     3421 ttatacacat atcataactt tgtacactta tcacaagttt gtacatcaat cgcaaattta
     3481 tacacttgtc taaaaatcac gagatttatt atacactgat attgtggctt gtttatgaac
     3541 atacacacct gtaattgtta aattaaaatt cacaaagata tctatgtgat acatatttac
     3601 gtgaataagc aaattgtttt aaacaaatat agtctgttca gcgaatattt taagccctat
     3661 acacattaaa ataactcccc caactatcct tgccttgctt ttgagttttc cgctgaattt
     3721 gttaccaagc ataaacccac agaacgataa ggccgccgta acgacacata taaacgccgc
     3781 cgacctgact atgtccacac caagtgccgc aaagcttatg cccacagcca gtgcgtccac
     3841 agaggtggca actccctgca caagcacctc cccgaaagtg agcctgcccc ctgtctggat
     3901 ctcatcttcc cccgaaccgc atatcatgtt caaccctatg aacccaagca gtatcaaagc
     3961 tatccagtgg tcgaacctct ctatgtactc cgcaaaattc atgcccagcg catagccgat
     4021 cgcaggcatt atcccctgaa aaagcccgaa tatgataccg cacatcaatg ctcccctgac
     4081 tctccctttt ctgacgcaaa gcccattggt gaccgatacc gcaaaagcgt ccatagaaag
     4141 tccacacgca gtaagaaaaa gctcaaaaat actcatatga tccccctgta taatgtataa
     4201 aaacagcttt acattcaatc ttgacttccc ttatattttt atctgcaccc acacttttta
     4261 cgcctgctac cacacctttt ctgtaggggc aaacaacgtt cgccagtcga agcaacaaac
     4321 ccatgcttgc atcaaacaaa atagccccca ctcaacaaaa aaacgccatc tccgtaaaat
     4381 acaaaataac atctttgcat tttccgaaaa cagcgtcata ccgttatatt ttgttaatcc
     4441 tcacactttg aagcaagaag cgaactgtat tgatttctga actgctcaaa tctgccctcg
     4501 tcaagagcct ctcttatctt ctccgtgagc gtgttgtaga aatacagatt gtgcataact
     4561 gccagcctgc cgcccaactg ctcctcagac ttgaatagat gacgaatgta tgccctgctg
     4621 tgattacggc aagcaggaca atcgcaatgc tcgtcaatag gtctggggtc agtttcatac
     4681 cttttattag tgatgtgcat aatgccgttc catgtgaaca gcgttccatg acgtgcattt
     4741 ctgcttggca ttacgcagtc aaagaagtcc acgcctctgt aaacagcctc gattatgttt
     4801 gtcggtgtgc caacgcccat aagatatctc ggcttatcct ttggcataaa aggctcgacc
     4861 ttttctataa tgttatacat tacctcagta ggctcaccca ccgctagacc gccaatagca
     4921 aagcccgaac agtattccat ttctcttatg cgcttcatat gctcgatacg cagatcctca
     4981 taagtacagc cctgattgat accgaacagc atctgatgct tgtttatagt ctcctcaagt
     5041 ccgttgagcc ttttctgctc agcgacacat ctctcaagcc accttaccgt tctatcacag
     5101 cttgcctttg aatagctatg ctcagcaggg ttctctacac actcgtcaaa cgccattgcg
     5161 atagtagacg caaggtgcga ttgtatctgc atactctcct cagggcccat aaaaatcctg
     5221 cgaccgtcaa catgagagtt gaagtacacg ccctcctctt tgattttacg cagcttggcc
     5281 aaagaaaata cctgaaatcc accgctgtct gtaagaatag gcttgtccca gttcatgaac
     5341 ttgtgaagtc cacccatttt gtatataacg tcatcgcccg gtctgacgtg caaatgatag
     5401 gtattgcaaa gctccacctg agttttaagc tcatttttta aatcaatgga agaaactccg
     5461 ccctttatcg ccgccgaagt tcccacgttc ataaaaacag gtgtctgtat agttccatga
     5521 acagtttcaa actgccccct gcgtgcagca ccctcttgtt taagtaatgt aaacataaat
     5581 tctccttata aattattttt tcagcctatt ccatatcata c
//
LOCUS       DAWEMR010000168         5558 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5631_length_5558_cov_8.660549, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000168 DAWEMR010000000
VERSION     DAWEMR010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5631_length_5558_cov_8.660549"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2751..2760
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5085..5094
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1452 a         1215 c         1118 g         1753 t
ORIGIN      
        1 cgcagttcac caagagataa ttttatcccg tttgggagtg ctgaaaatca cctgttttgc
       61 gtcgtttttg tcgtttcgat tttgcctaaa atagggtgtt atttaggcgg taactaacac
      121 gtaactaaca ggtaactaac aaaaaaataa ggcatctgtt ctttgaacgg atgccttttt
      181 tgataaaaac attgtcaaac ctgtaggcga tattgaactg tatattgtat aaaaaagctg
      241 agaccgaagt ctcagctttt tcttatatct tattgatcgc ctccaaaagc tcttccacat
      301 ttacgtgtgt atagaccttt tcagtaaccg acattgcgcc tgaatgtcct acaatcttct
      361 ttatcagtgt aggattgaca ttctttgctg ccatcataga aatacaagta tggcgtgtat
      421 cgtggggctt gtgcgtgcat ccgatgcgtt tcatgatagg agtccaatag gaatcgtagt
      481 agttgcgata tgtaaagtga cctccatcgg gtgtgtgcag caaatactca cagccctctt
      541 cataccactt ttggaatatg ggataggtct tgtcatgtat cggaaccatt ctgatgccac
      601 tgctggtctt gctatgcaca acgttgaaac aatgctcgtc tatgtgaaca tcctcacgtt
      661 tcaagtcgag cagttctgac acacgcacac cgctgtagat cagcatcagt acgatctgcg
      721 catagatatc gtccttctgc atccaaagag cctggatctc gctttctgtg aacggcgtgc
      781 gatccatttt gtttggattc ttgttcttga atttctggat attcacgtac tgtgaatagt
      841 ccttgctgca aagctcgtac ttcatagcat agtcgtacat ctggttcagc agcaccttca
      901 atttacgaag tgtgggataa ttctttccgc aggtgtctac aacgctctgc agatccatca
      961 gtttcagatc cttgaatact cggttatgca gcggctgaca agccttatag gatgcctgat
     1021 agccctttac attcgattcc gagatcgtcg gaaatttctc agatgcccat ttgtcgaaca
     1081 cctcagaaaa agtgatcttt gataccgcaa tgtcatatgg atttttgttg tactccataa
     1141 gcatctcaag accttttgct cttgtcggag cataaccgat ggtaacgcag acctgtttgc
     1201 acttgcctgt ttccacatcg atctcccatc cgactgtttt gcgtacgata tacggattac
     1261 gtcttttgcc ggacagctta taaactgtgc caacgccgtt tcccagtttc attggcatca
     1321 tctcccttct ttgccttaaa caactttata ctacgtcgtc attgctcata tccttaaaat
     1381 agccggtctc ctcccatacc tttttgtctg ggcagaagta attgtattcg ctggatcctt
     1441 cttgtttgag ggcatatcca aattgaagga tacgtttctg aatgccaatg cgtataaact
     1501 gctggctctt gccggttgct tttgcgattt cggtgatagg gacgtttcgt ccagtaaatt
     1561 taagtgtttc catgttaaat tcctcctttt agatatagcc agattattct gtcataacta
     1621 tagtatacaa ctgaaataat agtgcctgtc aatagaaaaa tcattctccc ctattgacaa
     1681 gtgcttatag gtaatggtat aattagacag agctaatgtt gaaaacttca acttttgcct
     1741 ttatttcgta cgcaattcct gctgccttca gtatgtcatt gaccgtactg atttgaatgt
     1801 tgctagtgag ctgtttgcca tcattgtaaa ccttggtgct gttgcaggca accttcagct
     1861 ttgctttgag gctgtcaagg cactcggcgt tcttctcaga gttcttgtat gtatcaaaag
     1921 cctctgcagc cttcgtcttg aagtcaagga ttctgtcata gtacaggaac atatcgtctg
     1981 agatgactgg ctttttgcca tagagttcaa ggacagcctg ggcaaaggca tgaggctttt
     2041 cggggtcttt tcgcaagcct ctgaggaagg caatctgata cgcaagcgcc ttttctgcca
     2101 ttgtatttac aacagggtgc tttactttac tgtcgtaata tacgagcgca ggatacttcg
     2161 ctgcatactc caatgccaga tccggatctg ctttgacctc tttgaccatt ttcatttgct
     2221 gctctgcact tgcaatcgca ttgccgatgt cggtcataga gtagtcagga ataagaaccg
     2281 tgatctctct gggattcttg cggttgactc tcgctctgcc aatcacctgc tttagtgtga
     2341 ttggatccag tgtttcgaca acgatcagat tcaattcatc gtcgtgcaag gacactccgt
     2401 tctcagccac cttggtggtg atcaggtttc gatctgaaaa tttctcattt tgagcgatct
     2461 ctgcaatttc tgcacgtttg tcttcatctg agtagatgtg ctgggtattg cctaacactt
     2521 cttgaagcac ttttcccttg ctgatatcat ttacatagat caggcttttg gtgccctgct
     2581 cattttctcg tttgatataa tcagcaagtt cctttgtgtc gtctggggtg tatagcttga
     2641 agttgatata gttccaattg ctttccatcg agtaaaccat cttcaatctt gttttgtacg
     2701 aagcatacgc atggaaaaca gattttacat tggtatccca gtccattgca nnnnnnnnnn
     2761 gcctgctctt gtcctgattc acattctatt tccgctatca caggagtaac cgcatctggt
     2821 gttgccgtga ggtaaattct agcggtattg tcacgatttg ctttcagata ccgaatgatt
     2881 ttctctgcgt acactgagaa attagcatct tccagcagac aatggacttc gtcaagtatg
     2941 aggaggtatt ttttgccttt atacttatac gttgtctcag caagttcttg ataggtaatg
     3001 gcgatgatgt tctctcccaa ggaaatgctg tcaagcaact ctgcaggaac atctagtcct
     3061 ttcgatgcct tgatatcgtc ctttttgaac tgacaaagcg ccatctttct gttggtcaca
     3121 tagatgaccg gcattgaggt gtgcttgatc atcttgatta ctgcgcttgt cttgccagaa
     3181 ccagttggag agacgataag cattgactct cccaagtttg gaatatcgtt cgaatcaatg
     3241 atctgactga tatactgctt tgaggtgcca actttataat cgccaatcaa acataagaat
     3301 cgtagagcaa cgctcggttg ctcctctact actttcagca ttctttcttc aagttcatct
     3361 ttttcatact tcggattgaa cggcatgata tacacaccgt cggactccat tgaagatgtt
     3421 tttgatttca agtctttaat cgttctcatc ataacatttt actcctttcg gtttatgaat
     3481 tagaaatggc tatgccctca tatccgtttc cctgatgctt tttgactttt tttacaacgt
     3541 atccattcga gagaaggtaa ttgtttaatg cctgtgaaaa gcctgtcaca tctttgaata
     3601 catcacagtt aaagtcgaga caatgctgga aaacatcgta caagtctttt gtcagcacga
     3661 aattaccggg catagatctc acataacact gcacaaaacg ttctatgttg tgatactgcg
     3721 cattgatcgg cgtgaattgc agagcgtagt catccagccc ggcatcgtca ccggcaaagt
     3781 gatagccatt tgctttcagt ctgaaaaacg cattcaaagc ctctgttgtg accgcattca
     3841 attcagaccc cagtatctct gaaacaatgt tcggattttg ctgacttttc gggatcttca
     3901 catcaaatgg cagtgtgcag attctgcgag caatggcgct gtcactgctg ctgtacgcca
     3961 taccaatatt gtgattactt gcgataatga ccttacaagt agacgtaaag ctgacctgat
     4021 tcgaattttt cttgtcaacc gtaagccggt catgtccaga gatgctcttt agctttgcca
     4081 gctgatctgt attgagttcc aaggcgcctt catcagagct aacgttgatc cgtgcctcct
     4141 gaatctctga cattgcaaaa ttgccgccaa gcagattttt catggataag cctttgactg
     4201 cagaaggact tagcaagctg cgtatcaggt ggataaaggt agatttaccg ttatctccag
     4261 aagcaccagg gaaaacaaaa aagcgtttcg catgagcgtc actgctgata cagtagccaa
     4321 tgatctggta tgctctctct tcaagcgtct tatctccagc aaacagagtt gacatgaaac
     4381 ggtccatgtt aggatgatac tgcgggccag atcccagata cgccccggta agctgaatgg
     4441 tcggaaagta atcagaaaca atatcaaaca atcctgtgcg gttattcaga aagccgttcg
     4501 agcaataggt atagcgttcg tcatttggct ctccttggta cagatcagct cgcatttcca
     4561 actcatttgt aatactgcaa atggcaggct gtgtgaactc atagccaagc gctgtaacat
     4621 cagaatacac tctgcatatg aagaccttct tattgtaagg aacatataca ttgttctcct
     4681 tcatgtaaaa gtggttttgg taccgccata gaggtttttg ctgattgagt gtgtttacca
     4741 ccagataaaa tatctcacta gcatttgcac aattcagtct ctggactttt ggctcagtca
     4801 cagcgttttg cagttcttga gaaacgacag gtggagttcc tgccacttct gaacacactg
     4861 cgcctgctct tccaaagatt tcaggacttt ttgccatctc ttcaagtgtg attggtacaa
     4921 acaatccgtt tccaccctgc acagggggta aatgagaatt tgtttcattc atcttgtttt
     4981 cctcctttat ttaattgtaa agatgccagc tgcttgagct gtaagtgtat tataacagaa
     5041 ctcaattatt cttgcaacac ttttacacaa ataatgataa atctnnnnnn nnnngtgcat
     5101 tttgtacaat gagaatcact tttttatcta aaccataaaa atatacggtt agaaaggatt
     5161 aaacatgaga caatttgatg tagttaatac aatcgttgat attgtatttt caggtaaaac
     5221 gacaggtaac tatgtagaat ctcaaacaga aacgacactt gactgcaaac cgaaaattta
     5281 tattgttcat ggaggtacac tacgtgatca cctaaaaaat aaaaaggaga cagcaagatt
     5341 aaccgcaaga gtaataaatg caatactgtg ccgtgtaaaa ttttacctct accctgactt
     5401 acaaactatt gacccaggtg aaactaaaaa atcacctgaa aagttaccca ttgaaccaca
     5461 tgactttgca aatagatgcg tcgccgcttt gatgacttta tttgattata atctgtcaat
     5521 gctgaaagta aaaaaagatg acaaaaacgc tggtgaaa
//
LOCUS       DAWEMR010000169         5540 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5660_length_5540_cov_10.204011, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000169 DAWEMR010000000
VERSION     DAWEMR010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5660_length_5540_cov_10.204011"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1297 a         1458 c         1135 g         1650 t
ORIGIN      
        1 ggcgagtatc ctgcaaaata acttatatct tatttgtctt aattttttta ctttctcttt
       61 cttgaaactg taagagctcc gctgataaga agtgcaagac ctgctacgca aacgcctgct
      121 gtcgcacccg tgttagggtt agccgcacct gcgttatttg ctgtctttga tgagctgtca
      181 gccgcctttg atgtgtcagc actgcttact gactctgcgg ctgatgaagt ggcagaagat
      241 gtatcctcag agtttgcaga agatactgtg ctttcggaag atacgtcaga tgagcttaca
      301 gagctttctg cttctgatga agagcttggt gtcttgagtt cttcaagctt ttcctctgcg
      361 gccgtaagag cgtcaacatt gcctacatat tccttaaggt cgccaagagc gtcataagcg
      421 gctcttgctg ccttgatgtc attctcgctg tcaagtgtta tctcgccaag gtcactgata
      481 agcttctcaa cagcctttac gctgtcataa tcctctgctg caagaactgt tacgtcgata
      541 gttgcttcaa caggtgaaac ggaaacgcct gttgtgtttg cagcaccctc gtttgtctta
      601 tctctgtaca taccatactt gtatgtaaca gtaacgaaat catcgtctgc agtaagagca
      661 gtatcgtcga tagtcacata atctgtaacg tctttcttcg caccattttt gagaactgcg
      721 taaaccttca agtcggaagc gtcataagct tcgccctctt tgcatactgt cttagcattc
      781 tcggtcacaa gcttttccac ctctgagccg acagccatta tgtagcctga atcgttcttg
      841 aagtagatag tgccgttttc gtctgcaaca aggtcagcta tagcgaactg tgcctgagcg
      901 cccgttggtg tgaaaagtga cggtgctgtt ttctttccgt ttactatctg agcgtcaaga
      961 acttctgtta cgcccttttt atcctttaca tagcggatag cgcctgctga tgcgttctct
     1021 gagaagtaaa catagttata gccgttctca ttcacaacgc caagacctga tgtctgtgga
     1081 tagccctttg tttctgcctt gtatgctatc tcaaagctgt caaggtcgat aactgcaata
     1141 acactgccct tatagtcgcc ccagcctgag ccgtttacgc caacataagc tcttccgtta
     1201 agaactgtag gagttgatgt gcttgcgcca ccaagtggga tgctgtcaag ctttgtgaaa
     1261 ttgccctcat cgtctattgt tgccttatag aaatagccgc cgttagatgt gaaataataa
     1321 gcgtctgtat ccttatcgta agaaatagtg cttcttactc ctccacctac tgtatactca
     1381 gtatctataa cgcttcctga gtttttgtca agagtaacta ttcttgcagg gtcggtagtg
     1441 tttgcctttg cattttccat gccgagaacg acaaaatcat cagttgcata tgcgcctgcc
     1501 caatagaaac cgcttttgtt tgtgaatatc cactttggtg acttctcttc atctgttgtg
     1561 tcaggatctt cgtccttaac atctatgcaa acatactgat cgttttttcc tgaaccatag
     1621 ttgcagaagc ctgtgtagat acagccgtca ttatagtaga tagggcttac agactgacca
     1681 cccacgctgt ccttgtatac ccacagcgac tcgagggtgt cagcgtcaaa tgcctgaatt
     1741 ccgccgttaa aggctgagaa tatcatgccg tctgcataag taacaggctg gatagcgttg
     1801 ctgtttgtgc ctgctcgctg tccaactact gaatccacaa cgcccgtttc cttgttcatt
     1861 ctcatgagct tgtcgccaga tgtataaacg atatcatctc caacgatagc cacaggcgac
     1921 ggaccgtcca tataggtggt atcgacctta gttgcccagt taagcactgc gttatccgca
     1981 ctgtcaggga ggactgatgt tgttacgttg ttgtttgttc cgtctgaaac aaatgcgctt
     2041 acacacacgc tcagactaag agcagtcgca agagcgaagc tggctgttct caaaattttc
     2101 ttcttcattc ttttctgtcc tttcgttgat acacactgtt ccgccgtcaa tgcacttttt
     2161 atgaacaccc cgaccacagc aaaaaaaatg cctttccgcc gaaacgaaaa agcattctat
     2221 ccgctttact gcctgcacag cgataaagca gaccgtcatc ttcatcgccc aagacagaaa
     2281 tagtcaacga aaacacagca gatacctgac ttatgacagc gcacaaaaag cctgtcaaac
     2341 acagtaatgg cggttgtgca ggatttggac ctgcttccct gacggcaagg cttttgccct
     2401 gacacgctct actcagctaa caacttttca aactgtgctt tcttaatatt cacatcatga
     2461 taattatacc atatcgctat ggattattca ataacctttt tacaccacta ataagtccga
     2521 tacaacgccg tttttgtggc attttcataa actcatagac taaaacttcg ttgggtatag
     2581 ttttaggcta taccctcagt ttctcctcca gctccgtgtc aggcttgaca gtgatagtgt
     2641 aattatttgt aaaaataacc atgcccggct ttgagccgtt tggctttttc acgtttctta
     2701 tgaaagtgta gtcaacgtcc accctggagg aatttcttcc ctttgagttg caggcggcga
     2761 ttattgccgc ttcctctatt gtcctgtcag gtggcatttc cccgtcgcag gtgacgataa
     2821 cgtggcttcc tgtgatatct ttgacgtgga gccaaaggtc agttttctct gcttccttgc
     2881 aggtgaggcg gtcgttctgt ttattgttcc tgcctaccct tatttcgtag ccgtcagaag
     2941 aacggtatct tataggcggc agagcctttg gaggcttgcc cttgaacttt gagcgcttta
     3001 catagccctc ctgaaaaagc tcctcacgaa gctcggctat atccccctcc gtctgcgccc
     3061 tcgttagaga atcgaaaaca gaatccagat acttgacctc ctgctcgccc tctgcaataa
     3121 gcacagtgag ctttttctct gcggtgtcga gctttctgta ctccttataa tacttctgag
     3181 cgttctgtgt tggcgtaagt cttttgtcaa gtggtatctt cactgtaggg cagttctcgt
     3241 cataaaagtc ctgcacctca gcatacgcca tgcccttttc aagtgcatag agatttgaag
     3301 tgataaggtc accgtaaatg cggtattttt ccctgtcctt gcactcttta agctcctcac
     3361 gctgattttg cacacgcctt tgagttctct cggaggtgtt tataagaagc ttgaaaaggt
     3421 cgttggcttt ctgctttatt cttgaaagag agtccctctc cgcatagaaa tagtccagaa
     3481 gaacgcttgg cgactcgaag ctttttgtca ccatgagcgc accatactgc gtgatatcgc
     3541 agaagcagaa atctttcaga ttgccctctt ttgttttcag cactgtgtag acgttagtgc
     3601 cgtcatttat gctgtcacgg actttcgaga aataaaacca cagcctgtcc gcagtgtcgc
     3661 cgctaagctg ttcggctgtt atctcagcac cccttgccgc aaagaacaca gcttcccttg
     3721 cgaacactgg cgaaatgccc tccagcgttt tcattattgc ctttgaaagc tccgccgtgg
     3781 ggttttcggc tatctttgca agaaattctt cttttgtaca ttccataaga ttcagccttt
     3841 gctctcttgg gggcaaagta tactgcgcac cgggaagaac cattctcact gatgacattt
     3901 cctgtcccac acgctttatg ctgtctatta tcctgccgtc ctcacgatag aggataagat
     3961 ttgaacgcct gcccattatc tccgccgcaa gtgtgagatt caccctgtcg cccatttcgt
     4021 tggtggcttc aaagtcaaag aaaagtatac gctcaaagcc gtcctgccta attgcagtga
     4081 gtctgccccc tgcaagatgc tttctcatga gcatacagaa cataggcggt gttttcggat
     4141 tttctatatc cgccgttgtt atatggaccc ttgccgagcc tgcacctgtg ctgaacataa
     4201 gcttctgcgc accctcttta gtgcggatag tcattataag ctcctctcgt gaaggctggt
     4261 gtatcttatc caccctgcca tcaataagag ggtcaaattc ttttctgagc aagctcagat
     4321 aaattccgtc caatgccatt ttacttacct gctttttata ttacttcaac tatatccctg
     4381 ttgcttacgg ctatctctac tctcagatcg ctgaacttgt ttttcagctc gttggcgata
     4441 aattcgcaga agatattttc cgtatagaaa tgacctgcgt ctataacaga aacgcccata
     4501 ttcccgccgt ctataaaaac atcatgcttc acgtcccctg tcaaaagcac ctgacagccc
     4561 ttttctgctg catcagcaag gaacgaaccg cctgaacctg agcacactcc cacacgcctt
     4621 atctcacgct cgccgccgct gaaacggact actctacagc caagaacgtc ctttgctctc
     4681 tgtgcaagct cacggacgtt catgccgtca cactcgcaga catatccaca gccaacgcca
     4741 ttctcttcat gcaaagcgct ctcaggttca agacccagca ttttgcaaag cacgtcattc
     4801 attccgccct ctgcggagtc gaaatttgtg tgcatggaaa gagcgttctt tcctcctgcc
     4861 gcaagtatca cagcaggatc gttcggcacg agggtcttta gtcctctgaa aataacaggg
     4921 tggtgggtga gaacaaggtc aaagtcacgc tgacaggcct ctcttgccac ctcagcggtg
     4981 caatccagcg ccaccagcac gctccttacc tctgcggaca tttctcccac gttaagaccg
     5041 ctgttgtcat agccctcctg caagcggtat ggggctaaat cctctataaa actataaata
     5101 tcataaactg tcgtcatttt tgtcagctcc tttttgtgtg tcaaagtctg tcaagctcgc
     5161 tctgtgcaag cttaacgagc ctttctccca gttccttttt gtcctcagac ttcatcattc
     5221 cgctgcctgc gacaaaaatt ctttttgcct gccttgcgat atactctttc ccctcgccct
     5281 caggcttaac aaagccgaac tgcaaatatc tgtcatcaca ttcataagac ggagttttgc
     5341 ctgtatacac cgcctgcatg acagaatatg caaacagccc ctctgtgcag gcaagctctt
     5401 ttttcacctc aaagccgttg tcataaagcc actttctaag cacgtcccat tttgtcatag
     5461 gctgtaaaac gaggttcacg ccgtttttca cccacggaca tttttcaaga agccttgcaa
     5521 taagctcacc gcccatgccc
//
LOCUS       DAWEMR010000170         5520 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5687_length_5520_cov_12.990119, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000170 DAWEMR010000000
VERSION     DAWEMR010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5687_length_5520_cov_12.990119"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2843..2852
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1665 a         1172 c         1153 g         1520 t
ORIGIN      
        1 cataaacgga actgttatat caaacatcag cgagctgaat gagataaaga acaagtgcaa
       61 agtcggtgac actgttcagc ttactatata cagaaacaag aaaatgataa atgttgacgt
      121 tgttctcggc gagtccacag aggacacttc tgagtcaaat gacaacgacc agaacaacaa
      181 taacaataat aataacaata acaacgacga ctacaacgat tactatggaa actatggcaa
      241 tggtcttggc ggctataatt acggctacgg tttctaaata acaaagcttt cttcatattt
      301 ttcatattct tttacttcag ggaaagagct gttccgagag atcgggacag cttttttctt
      361 gactttcttc ttgacagagg gtcaaagcag tgttattata ataaggtata ctattaaggc
      421 aaagcccgcc tgacggtatt gccttaaacg agtacacttc tttattttgc aaaggtcggt
      481 gattttatga caaaagaaga atatcttgac ttttgcggct ctatcgcagg tgcgacatat
      541 gaccagcctt tcaacgagga ctttcagtcc actgcactgc gccactggga cacgaaaaag
      601 tggttcgggc ttttgatgct ccacaatgac aggtggatag taaacttaaa gctaaaaccc
      661 gaagaagtgc tgttttacag ggatatctac aagggcgtga cggaagctta tcacatgaac
      721 aaggtccact ggataacatt gtatctcgac agcgacgtgc caacagagga gatagaagat
      781 cttaccctca gaagctttga gcttacaaag agtaaagata aaaagaaaac caaaaagcga
      841 aaggaagctt tataaaatga gaactttttc aaaatcaagc aagcttgata acgtgtgtta
      901 tgatataaga ggtcctgtta tggacgaagc aaatgaaatg atagcaaagg gagagaagat
      961 attcaagctc aatatcggaa accctgctcc gttcgcattc aatgcccctg acgagataat
     1021 acaggcaatg acagacaacc tgagaaacgc tcagggctat tccgactcaa agggcataac
     1081 agaagcaaga caggctattt atgattatgc acactttaag ggcatacctg ttgaaagtct
     1141 taacgatatc tacaccggaa acggtgtttc cgagcttatc acaatggtaa tgcagggtct
     1201 tctcaacaac ggcgacgagg tgcttgttcc tgcacctgac tatccgctgt ggacagcttc
     1261 cgttactctt gcaggcggta ctgccgttca ctatatttgt gatgaaagct ccgaatggta
     1321 tcctgacctt gaggacatgg aaaagaagat aacagacaag acaaaggcta tcgttatcat
     1381 caaccctaac aacccgacag gtgcggtata tccaaaagag atacttgaaa agatagcaga
     1441 catggcaaga cgtcatgagc ttatcatatt ctcagacgag atatatgaca gacttcttat
     1501 ggacggcgtt gagcatactt ctatcgcttc actttgccct gacgttttct gcatcactct
     1561 taacggactt tcaaagtcac atcgtatcgc aggcttccgt gtaggctgga tgacccttag
     1621 cggtgataag agcagagtaa aaggctacat agagggtctt aatatgcttt caagcatgag
     1681 gctttgttca aatgtaccga gccagtatat aatcaaatat gctcttggtg attacacaaa
     1741 aactgacgat ctgctcctgc cggggggacg tatctacgat cagagagagt atatctacaa
     1801 cgcacttaac agcattgacg gtctgtcggc tgtaaagcct aaggcggcat tctatatctt
     1861 ccctaagata gacgcaaagc gtttcaacat cacaaatgac gagcagttcg tgcttgattt
     1921 tctccgtgag aagaagatac ttcttgttca tggcggcggc ttccactggg aacagcctga
     1981 ccatttcagg atagtttatc ttcctaagct tgaggatctt gaatactcca tgaatgaaat
     2041 gagagatttc ctcaaagact acaagcagaa gtaaaactgt agagcggaca gtgttcgccc
     2101 aatatgcttt tatgctcata ccatacatct acaaatacag acacgaggta caaaaatgtc
     2161 aaaactttgc aaatcatgtg gaaactatta tgagggagat cactgcgaca agtgcggcta
     2221 tggcgaccca aacttaaaga cccacgccgc tgacaaatac aaaaagccca caaagcctga
     2281 acgtttcagg acggaggagg aaaaagagct ttacgccgag tgggaaaagg agaaaaaaga
     2341 ggacactgag ataaagcgtc actatgatcc caacgcaggc aaaaagaccc ttgccattgt
     2401 ggcggtagtt gttataggcg ttatcgtgtc tgtgctatac aaaagcggag ttattttcag
     2461 ctccacaaag acagatgtaa tagaaaaata cttcaagtcc atacagacaa gcgatttcga
     2521 ctcattcgtg gagtgctttc cgaaagagat gaagaacgaa tacgaacagc agcgaaccga
     2581 aggcggatat ggcaaagagc aggtcatgaa agaagtcctt tatggtgatt tcattgatag
     2641 ctatggttct gattacacta ttgacgtatc tttcggcaga gaaacaaagc ttaaaaaatc
     2701 tgaatatgac ctgagcgaat acaaaagctt ttacggctct gcaccgaatg tttcagaggc
     2761 ttatgagata gtaacgaacg tgactttcaa aggctcaaag ggcagtgagg acgcaaagct
     2821 ttacctctac gttggaaaat gcnnnnnnnn nnggaaaatg cagcggaaaa tggtgcattt
     2881 tcaatatcac gcaggataac ggcattgttg acgaggacac tgggcttaaa acttcagaaa
     2941 attaaatagc acaaagcaaa accgccacgg agaaaaatcc gcagcggttt ttttgttttg
     3001 cgataaaacc tatatggtgt gacgtcgtat ttctgttaaa cagaaattga gtcgctcacc
     3061 tacatataag cggcgtaatt atacgctttt atgtgatatt atctcttctt aaaagcaaat
     3121 gactttcttg ttctctgctt tccgcctatt gacattgtga atatcttgtc gctcatgaag
     3181 attttcagtg cgacagtcat gcacactaca agaagcacta tctgatagat aagtccccct
     3241 gcataaaggc tgtagttgcc aaacattacg ttctcgcttg ccataaaggt atgggtgaac
     3301 ggtatagcat agacgatata ctttataaca ggtgaaagcg ttctgatatc aactaccatt
     3361 gcaagaagat atggtatcat tgcggagaag gttataggca aaagcattgt ctgggcagat
     3421 tttgcgtctt ttgcaagtgc tcctaatacc attgagagtg acaatgagat aaggatagac
     3481 acaaacatct gtattcccac aagaacatac tgtcctatgc tcatagtaag accaaggttt
     3541 tccattgcag attcatatgc tgaggtatct cccatgccct cagtaagacc gcctgtcatt
     3601 ttgctcatgc cgaacatata gaccacagcc tgcaaagccg caacaacacc tgcggcaagc
     3661 atttttgacg caaggactga aagtcttgaa acaggtgcgg aaagaagcgt ttcaagggtc
     3721 ttgtctattt tctcggtaga gatcgcgcta agtatcatct gagccgagaa cattataaga
     3781 acgaacatta ttataggcac taccatgctc tgtgctgagc aaagggacat cactacagaa
     3841 ctgctcactt tatctgcctt atcgccaaca accgttgttt cagaaagcag tacagggtct
     3901 tcaagctgat tcatttcgtc ctctgtcatt gcgccgcttg aaagcttatc ctgatagata
     3961 gttgatttta ctgcctgctg tataactgca agggcagtgt ctgagcctgt gtttatattt
     4021 gacatggtcg caagagaggt cattctctga acatatccaa cgtctgcctt tttatgctgt
     4081 tttacctgct ctgtaaaacc ttttggtatt atcacaacgc tgtcctgatc gagcctttta
     4141 agctctgcgg catagtcgtc agactttata tctacaactg ttatcttgcc gtcaccagcc
     4201 tttgcggtag ctttcagaga gtttaaaacg ttttctgtga actctgtttt gtcaaggtcg
     4261 cagatagtaa ggtcggaagc cttttcggca ctgtcgctta ttgcccctga cattgcctga
     4321 cccgccataa caagcaccac tactgtaact atcattgtta taatagtttg caggctgagc
     4381 atttccttta attccttgcg gaaaagacta taaaacttca cggtcaagca ccaccctttc
     4441 aaagacttct tcaaggttct gagcgccata tttttcaata agctccgcag gcttgccgac
     4501 ttccagcaac tcgccttttg caattatacc cactctgtct gaaacatact ctatttcaag
     4561 catattgtgg gaagaaagca gaaagctcat gccctcagaa gcaagctttc ttatcattct
     4621 tcttatttca agggcgttta taatatcaag tcccgaagtc ggctcgtcaa gtatagccag
     4681 tgcagggagc ggcattatcg cccttgcaag gagaagcttt cttgccatac cacgggaata
     4741 atttgatatt ttatcgttaa gcctgtcacc aagctcgcat atctcacaac cacgcttaac
     4801 aaactcgtaa gccttttctc cgttgtcggc atagatagaa gccatgaact ccagatattc
     4861 tctgcctgtc atggtcttat acgcaccagc ctcgtcaggg aggtatgtaa tgctttttct
     4921 taccatatca ggttcagtga gaatactatg acctgccacg atagcgtcgc cctctgttgc
     4981 ctgcaaaagt gttgatatca ttcttattgt tgttgtcttt cctgcaccgt ttggacctat
     5041 cagcgcaaat atctcgccct ttccaacgtc aaaagtgacg ttatgggagg cgtaaatgcc
     5101 ttttttgtac tgctttgaaa gccctttgac ctgtaaaacg gtttccatcg ttttcccatt
     5161 cctttctttg tctgtatcat tgtatttttt gtgaataaat caagtatagc acaccacgtt
     5221 ccaactgtca attgacattt gtcatgcact gctatgtaaa tatgatttac taatagtata
     5281 ctctgtctat ggcacgatgt caatagcggt tttcaccaaa acaggaatga tttttattat
     5341 ttatgcgtta gaatgtaaaa ttcacttact ttgtgagata aagtaaacca cgttacacaa
     5401 tgattacaaa tcaaataata ttctgtacat tttcaactct ttcaagtacg tttttttgga
     5461 tatcacaatg taatgatttg attacatttt ggttacagac aagggtaata aaacggacga
//
LOCUS       DAWEMR010000171         5481 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5739_length_5481_cov_9.900663, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000171 DAWEMR010000000
VERSION     DAWEMR010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5739_length_5481_cov_9.900663"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1516 a         1298 c         1076 g         1591 t
ORIGIN      
        1 ctacccctcg tatgtgtgag aaaatgcgaa aagaggcgaa cggtgaagag tgcaagcgtg
       61 aggcaaaaca aacgtggagt ttggtaacta tcagcgaaat attgtcaaac gatttctata
      121 taggcaccct ccgtcaaggc aaataccgcc gcaaaaggat aaacggcgat gaagtcaagc
      181 aacacgagac tgaccacatt gtatttgaaa acaaccacac gcctatcgtg gattacaagg
      241 tgttcgcatt ggcacaggag aatctcaaaa aacgtaccac atctcattat cgtggcgtaa
      301 gaaaatacga aaatgcttat tccggatttc tctactgtgg cgactgcggc tcaccaatgt
      361 tctcaatgag ccgaaacgat attgccgccg cttatacctg cggaacatat cacagacgag
      421 gaacaaaagg ctgtacatca catcacacaa gggtcgatat gcttgataag ctgctaaaag
      481 agtacattca aaaagtccgt gacaattcgc aggatatgat cgaacgtctg caaaactcca
      541 ttgctaagga gcagaacgca agcaataaca gcaagtcagt cgtggagctg ataaaaggac
      601 agatcgagga cgcaaaagct gaaatgaaaa tgctcacacg tcagcagaca agagatgtaa
      661 tgcgtcaccc tgagcgtaca gagatcattg aggaaaccta caccgaaatg atcgacgagc
      721 ttaccttgcg aatagaggga ctgaaaaacc agttgcaact ggcggtggat aaaaataatg
      781 ccatagtaag agtaaaccga accgcaaaaa ccgtatttga aatttttgac aatattttga
      841 ataagcctac ccttgacaaa aatgacataa atctgataat cgacaaaata acagtttatg
      901 aagatcatat agatattcag ctcaaagctg atattgactg cctgctaaca tacggtacac
      961 ttgacaaacc tgagagcgta tcagcaatgg cagatgtcag cgaaaaagcc gccgaaaatt
     1021 ttaataaagg cactgagagc aaaaagtcag ccggacaact tgccaatgcc ataaaaaaca
     1081 gcaaaggcaa ggttcttagt gtcaacgtta tcagtaacgg cgaccccctc gagatctaca
     1141 ccaaccccga cggcgaggtg atattcaaaa aatattctgc cataaacgaa atgtccgagg
     1201 gcgcagctca ggcagcggag gtcatctcaa agcttggcgg agcgcctgcc gtggtgttcg
     1261 ataaggacca cgtcgtggca gtgtcaggcg tgccgaaaaa agagtattcc cagcgccgcc
     1321 tctcccctgc cctggaggag cttttggaaa accgcaaaac ctttgactat accgatacca
     1381 cagccgagcc gcttagagcc gtcgagggta tcaccaccca cgccctcacc atcgccccta
     1441 tcctcacaaa cggcgatata acaggcgcag tggcattcat ggctaccgac gataccgagc
     1501 tttgtaccga taaacaatcc atgctcgcca aagccgccgc aatgttcttg ggcaaacaaa
     1561 tcgaagaata ataccaaaaa ggaccgccgc ttgaccgcag cagtcctttt ttctgtacta
     1621 ttatatcagt cattgcccca tgtcctccgc agccaacata ataataaacc ctccgaccca
     1681 tacaaatgga tcggagggca aactgcagac cacactgcgg tggagctgat agtcggactc
     1741 gaaccgacga ccatgggctg tcgataaagc gacgtcatat cgtattttga tttttcgtgt
     1801 gtaaccgctg tatttgcacg gttatgccgc cttggataat tcattctccg tgccgagttt
     1861 tccgacaaat ctgtaataaa tctcaatctg ctgaaatgtt cttccgtcct cagcttgttc
     1921 tttgggatga acaacaattt tgtctatcag ctcatttagt attgcggcat ccaacttgtc
     1981 gattatggtg tattttttta taacgttaat aaagttatcc gcacaatctg attgtctttt
     2041 caactcgtca attttcgatt tgagaatagg taacgccttt ttgatttcca cctgttccaa
     2101 ctcgtaactc tgcgacatag actcatatct ctcatcgcct atcctcccaa gaacattgtc
     2161 ctcataaagt cggctgataa tcttatccag ttcctcacag cgtttttgct tttgctcata
     2221 ttcgagtgtg atttgttccg cttgcttttg tgaatcagac tgatatttcc gacttaaaat
     2281 cgacttgaat ttatccgtat ttttgcggta ctgaaacaag ttacgctgaa tgtcgatcag
     2341 tacaagctcg taaattgtat caaacgtaat gtagtgcgaa gcgcagtatt ctttgccgtg
     2401 agttttgtac atccaacagg agtacgctcc gtgataactg ccgtctttac gctgtttctg
     2461 cgagtaagtc aggcaatgac cgcaatccga acagtagaga agtccggcaa acatctgaac
     2521 ctctcccgcc tttgcaggac ggtgcttggc attcagaatt ttgtgtaccg tatcccacag
     2581 ttcctgcgta atgataggct cgtggcagtt ttcaaccacg atccaatcct ctttaggatt
     2641 gcgtaccttt tctttacttt tcatggattt gtttctctgc ttgccgtaaa caagcttgcc
     2701 tagatagact tcattgttga gtatcgcgcg gattgaagta acgtgccagt caaattcctt
     2761 gcgccagtaa tccgatttga agtaatccgg attgttgagg ttgaagtagg caatgggagt
     2821 tagtaccttt tcttctctaa atatttttgc cattttgttg tagccaacac cctctgatgc
     2881 aagtctgaaa attcttttaa caacctgtgc ggcaggctcg tccacgatta aatgatgacg
     2941 gtcatttggg tctatcttgt agccgaacgg agcttttgat ccgataaact gtccgtccct
     3001 cgccttagct tttttcgccg ctttggtctt ttttgaaacg tcacgagcgt acatttcgtt
     3061 gactacattc tttatcggga acagcaaatc gtcgctgtac acaagagaat catagttatc
     3121 gtccgccgca ataaatcgga tattgctgtc ggtaaaccgt tgaacataca tacctgcctc
     3181 gacataattt cgtccaaacc gagaaaggtc tttgacgata acaagattga ttttgccctc
     3241 atcaatgtcc gaaagcattt ttttgaagga cggtctgtca aagttcgttc cgctgcaacc
     3301 gtcatcacaa tagaatttgt agtctgtaat tttatgatct ttgcagtact gctcaagcag
     3361 aattttctga gtttcgatag aaacacttcc tccaaagttt ccgtcatcaa ccgaaagtct
     3421 gcaataaagt gctgccttgt aatgctgctg ttttttcata gtaactcctt tctcagcagc
     3481 gaatttaata agccttgcgg tatcttccgc tccacaagta tatcacaact atgctgtaaa
     3541 tgccagtaaa atccgctgtc tgtaaaaata tttgttgaat gttgctaagt ccgattattg
     3601 aaattttaca ttgttactaa ggagctgtta ttgatacgct ctattccttt ttgaaattca
     3661 tcgactacct ctccgacctg ttcgcctgat tccagtttgc gaaacattag tctgctgaga
     3721 ctatcagcta cattggtttt accctcaaat gcagaccaca cacgataggt cacaccgttg
     3781 atgtagtgat cggtatattc agccgactgc acctctgtgt tgtcctgaca cagaggttta
     3841 tcaaggctgt atcttttagt cttttcttct gccattcgct tatcacgctc cgtttcatta
     3901 cgtcgattgt taattttcgt ataaacaaat cagttcagtc ctttcatatt taaattttga
     3961 tggtgctaat tagttacgca ccatcttaac tgttttttaa tatacagctt ttttcttgtc
     4021 gtaaattcac attttccaat tacaacgcct gaaaatatgc cgattttgtg cagtcttctg
     4081 cacttacgac ccctgaatca ttcaaagctc aaatttatga ccccataaaa attatgcaca
     4141 atcattttgc tgaaactgcg tattttcggc acttttgcgg cgagctttgc tcgtccgctg
     4201 tgactgtttc gggggctatg accccgaaac tttaacttgc aatgaaatat ttcactttgc
     4261 tttaactgct gcatatccgg tatttgtgca gattgacaac tgccttttta ttatcagaat
     4321 taaaacctct cccacatatc gcccacagcg gctcacaaat cgcttgtgtc ggcttttgaa
     4381 atgcttttgc taaacttacc cgacaaaaaa tccaagcccg caaatcggct tacgtttgcc
     4441 gattttggtt gacagtaact ttgcccgttt tcagttactg tcacacctgc tgtcagcacc
     4501 gtaaaccaag cattttcaag ggtttgcgag gtattgctta tgcactccgt tgacagtaac
     4561 tccgctacca tttttactgt cactactgtc acgctcacag tcgtaatgaa gaattatcag
     4621 tctgccgttt cttgttcgct tgtactgaaa atcaattcca aactttttga gagtgtaacc
     4681 gttctgcacc agatctcttg ttactctgtt gggataaaac gtttcatcgg aaacactttt
     4741 cagaacattc actaattccg aaggtgtacc gcagaaatca atatgcttct taagatacac
     4801 atacagttca gccagaaaaa tgttatcttt atttttgcag ttgagcggct catcggtgac
     4861 gatccacttc tttaaattgc tgtcaaactg caaacttatc tcggcatttt caatgtcacg
     4921 tcctacacag tatagctttg ctgtacgact tccacgcttg ctttcaatca gaaccatgct
     4981 tccgtcaaca caaccgctta agcctgtact gcctgagatc atattgaacg gatcgctgtc
     5041 agaacacttt cgtgtgtgat gcacaactac tatcgtaatt tcaagcttgt cggcaagtgc
     5101 tttcagcaca gacagttctt tgtaatcagt accgtagctt gactccgatt cgttacgcac
     5161 cttttgcaga gtgtcaatga acacaacttt taaatcgtaa tgctcatttt taaagttttc
     5221 aatctcttcc tccaaaccgt tcccgattga ttcggacatg attacaaaat gcaatcgctc
     5281 tgtaggttcg tcagtgattt cgtaaagacg attttgaatt cgtacatagc tatcttccag
     5341 acacaaatac agagcagttc cttgtgtagt ttgctgacct aatacctttt ctcctttcgc
     5401 aatggacaag cacatatcaa gcgcaagcca cgattttccg actttcggcg ctcctgctaa
     5461 gatatacaga ccttgtgcaa g
//
LOCUS       DAWEMR010000172         5478 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5743_length_5478_cov_7.683386, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000172 DAWEMR010000000
VERSION     DAWEMR010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5743_length_5478_cov_7.683386"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1650..1659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1440 a         1167 c         1030 g         1831 t
ORIGIN      
        1 tccatatcga ggaaatccac gggctttgtt gcgctttttt ttacagcgcc ttttttattc
       61 gatctcttcg ctgttgtcag tatcagtgct tgtgatgata tttgttgcat atgtaagtgt
      121 aagcaggctg tcacccaaat gaacattttc aacaactatg ttttcatagt cgatgctaag
      181 atcataatgt gaaaaattcc agaacgcttt cagaccaaga tttacaagct ggtctgcaat
      241 agcctgagtg tttgccttcg gtatacaaag cacagctaca acaggggagt ttgcttcgca
      301 aaagctttct atttcattag tgtgacgaat cggtatgttt cctaccactt caccagtgag
      361 cttagggttt atatcaaata tgccaatgag attgcaaccc cttgtttcaa agttaatatg
      421 tgtggcgata gctttaccaa ggtttcctgc accgataagt atggtcttga aatgtttgtc
      481 tactccgagt atcttgccta tttccattct aagctcgtca acgtgatagc catatccctg
      541 ctgaccgaat ccgccaaagc agttaaggtc ttgtcttatt tgtgaagccg tgagcttcat
      601 tctctcagaa agctcccttg aagatattct ttcaagtcct tctttttgaa gctcacctaa
      661 aaatctataa taacgtggca gtcgttttat tactgatgcg gatattgaac cgtctttaga
      721 catatattta cactttcctt tactaataaa catgattgat aaataaataa caatatcaca
      781 taatataata gcactaatct tttcttttgt caaggacttg taaactctct gcggtcttaa
      841 aaaaagcagc ctcgttttac agaaactgcc ttatgtatat taattttatt ctagatcttt
      901 tttctttaca gaagcaacac agaagtcgat gcgttcttca tcatcttctt cattccctga
      961 acctgtgaga ggaactcctt ttgttggatc gatcccgtca acggctgtaa caagtcctgt
     1021 gccctcgcca atagaagcac ccggtactct gaactttgta atgtcgatag gctcgataga
     1081 ggtctcaaca ggcttttcaa gctgtggtat ccactcgtat ggtacgcgat aagctctgta
     1141 gatcatgttg cttttgaatg ttccagcaaa attcttgaaa taagggttta caaattccca
     1201 aactatttca tggtcaggtg tgacctcaag aagatgtcca tccgaacctt ccgtaataag
     1261 agtgtttccg ttcggaagtc gctgtgcaga gctgatgtat gagctataga acttagaagc
     1321 gtcagtaaaa agcagatttc ctgcttcaag gggagtgtac tgccatgtta tttcaagtgt
     1381 aacaggattg aattgaagta ctcttgagta atctctgtgt gcattgttca cgcctgtgag
     1441 ggaagcaggg ttaggtgtgc cgtagccacc ctcgccaccg ttatcgaata ccaacaggtc
     1501 tccctctcca ggaagtccct ttggtatcat gtgcaggtgg tgctgaccaa ttatccagcc
     1561 aagcttcttt gtggcttcgc tttcgttgaa gttaggacct acacgccata ctatcttacc
     1621 tgttgatttc tcgataattc cgagtatgtn nnnnnnnnnt gtagagaagt tgtttatgtg
     1681 catccaatct ccacctgcct caccgtgaag accagggttt ctgaacaatg cgttctttgc
     1741 ggcctcgtca aagccaagct catcaaagtg gtcgcttgca cgccagctcc aaattatatt
     1801 gccttcccaa tccacttcga tgatcttatc gtcgataagt cttttgtctg aaatatcgtg
     1861 gttgtagagg ttctcgtgag taaggataag tgtattgcca ctgtctgtct ttggctcacc
     1921 atttggataa tagtaaccca cagtgctgcc ctcacgctga aaatcatggt gctgtctagc
     1981 catatagacc ggttctttac ccgggtcggc aacaagttca gttttgtcga atttccatac
     2041 gatatgaccg tcccagtcta cctgaacaag atcaagttga tcctgatatg catatttacc
     2101 tcctcttgct cctgttgtgc ccattacata tccgcctggg agtattttgt taggaaatcc
     2161 tcccagtcca gcccaaagct ttacttcatt gccattcata tctatgagca gagcaccttt
     2221 agcggacggg aagatcgtat agccgctgta ggctttttct ttgttgtata ttgtaactcc
     2281 tgttggataa atgcttggat aacccatgtc tgattcctcc ttgtttattt gtctgctttt
     2341 gtcaggtctg ccgtaacgtt atctggaaat gctttttttg ctgcatctgg ataataaaat
     2401 tcccttatgt tgtaactgtt tggatattta ccattctcat acgcccactt atttatttca
     2461 tcaagatgag atgcagcttc ctcagaaagt cccagtttaa tactgtactg ttcccagttt
     2521 gttttgaaat catctggttc tattccatat ttctttgata ttttttcagc cgactcatct
     2581 aagttgtcat ttatgtattt aacgctttca tcaagtgctt taagatagtt cgtaagagtt
     2641 tcagtattgt tctttgcaaa gtcgcttgat gttataagat aagcagatac gtttatccct
     2701 atatcttttg cagatgcgac ctgagtccag ccctctgctt catatttttt tatagctgag
     2761 cctgtaacga tagcggctga tgcctcaccg ttatgtgcaa ctgctatctg agttgatatg
     2821 gaatctgtgt tgacgttttt ctgctttgac gggtcaagtc ctaaataagt ctgagcctgc
     2881 cagttgaagt attctgaaac tgttccgata gaagttatcc atccttcact ttcatctaaa
     2941 gcatcaagat catctttgta ttttggtgca acaaaaagac ctccattata tgaaacgcta
     3001 gaacttagat ctgaaaaaat cacaagatta gtgttatcga gagtatttcc aattctgttt
     3061 acagcagcaa aatctgcaag cagtcctgta tctgctgtgc ctgttgcaat agcgtcaact
     3121 gtgttaatgc cggcagcgta ttctgatgtt tcgagagata taccatattt ctcaaatatt
     3181 ccttgctcca taccgatata gtcggcatac tgatctggct gacctgtcat gttcgcaact
     3241 ctcaaaacgc taagctcttt accgtctttg gtaaccttct ttgcggtttt tccgcttgat
     3301 gaactaccgc atgatgcaag aagagttact gtcaatgctg tcgatgctat tattgcgatt
     3361 atctttttta tgttcatttt taaatctctc ctgttaaatt aattatttta cgtcaaagtg
     3421 tttaagtttt ttcttgccaa taaactgtag gatcctgtca gcagcaaagc caatcatagc
     3481 aagtgtgacg attcctgcaa aagcccagct tgttttgtaa taaagcttcg agctgtttat
     3541 aagataacca aggccatcac tgccgaccag catttcggct gccacaacac agatgatagc
     3601 tgagctaagt cctagtctta ttccagtgta gatattagat accgctgatg gtacaactac
     3661 tgtgaaaaaa actcttgctt tttttgcccc catgcaggaa gctgcttcca cgagctgctt
     3721 atcagttgaa gccacacccg ctatagtgtt tacgaacata gggaagaatg atgcgtagaa
     3781 aataagtgct acttttgatt cttcgcctac tccgaaccac attagaaaga gtgtcgtcaa
     3841 tgcaatagcg ggcacaaatc ggaaaaagct aagtataggt tcaacgagcc agcgtacagg
     3901 tctgaaattt cctacaataa gacccatagg aacagcaaaa attattgaaa gtagccagcc
     3961 ttttattact cttccgacgc ttgcaacaat gtcactccaa agagttccgt ctttgataag
     4021 ttccttcatg ctgtcgatcg tttcctttgg gcttggaaga aaatattcat catatttcaa
     4081 agatcctatt ccccaaaggg caattattgc tacccatgat atgatgccac attttagtat
     4141 cttctgtata gcagaggact ttgttttatt attttccatc tttaatgtcc tccttattaa
     4201 atatagtaca gctctgagtc atcaagagct ttgtttacga gaatatttgt agtgttttca
     4261 tcaatagtgt aaagcttctt tatgagtact tttaccttat cgcctttttt taagcgggaa
     4321 gcattaagtg gatatcttcc cgttattgag atattgtcga tcagagctct tacttcaaaa
     4381 gagttacctc tgaatgatac atcttctaca actgcgtctt ccatagagct ttcaaatggg
     4441 tggtattcac tgtcttcaaa tttatttatt tcaataaatt ccggtcggat tatagctgag
     4501 gcattatttt ctgtaacttc aaaacccttt agtttgctgt aatcctcgat aatagcggac
     4561 tgaccgataa actgagcaac aaaaggagtt ttaggttcaa ggtatatctc aactggattg
     4621 cctatctgct caactctgcc tttgtttgtg ataacaattt cgtctgcgac ctctattgct
     4681 tcgtcctgat cgtgtgtaac gaaaatgcta gttatgccta ttttatcaat tgtatcacga
     4741 agccatgttc tcagttcttt tcttaccttt gcgtcgatag ctgcaaaagg ttcgtcaagt
     4801 agaaggagtt caggctgagg tgctagtgct cttgcaagag cgatacgctg cctttgacct
     4861 ccgctgagct ggtcaggata acgcttgcca acgtcaggca agccaacaag ttcaagaagc
     4921 tcagccacac gttgtttgat aaacttctta tcttttttct gaaccttcag accataagct
     4981 atattatcgt aaactgtcat aaaagggaag agcgcatagc tttgaaaaac aaatccgata
     5041 cctctttcat ttgatggaag tttgttgaca tttttaccat ctataataat atcgccgcta
     5101 tcctgttttt ccaaacctgc aagcatacgc aggatagttg ttttaccgct gccgctcggt
     5161 ccaagcagac cgataagctt tcccttttca ataccgaaac ttacgttatc cgaagcttta
     5221 aaatcgccaa agcttttatt aatgtttttt aattctatat acatagctga caactccaat
     5281 taagatatat tactgtgata gttccagtgt caacatcgct gcattcgctt attgatcttt
     5341 gacaatttaa gctcctcact ttcaattcat atcaagtcga tatgattagt atgcttttat
     5401 tatacatgat tttatggaaa tgtcattagt tatgaatgat tttcgtgaaa tcatacatag
     5461 atgttaggaa tactttat
//
LOCUS       DAWEMR010000173         5473 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5747_length_5473_cov_18.271133, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000173 DAWEMR010000000
VERSION     DAWEMR010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5747_length_5473_cov_18.271133"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1625 a         1088 c         1004 g         1756 t
ORIGIN      
        1 cttttccgtc aacaatcata tacttactga agtgtgtagt ctccacactt acagttgaat
       61 tctcctcgtc aagaatggta tccagctcat caaaattgtc attttcttca tcataccata
      121 aaaacagtag gttgtcaaac tcagtatcac caagcttaga cttatcgatc acatatgtaa
      181 gcgtagcctt atcgaacttc gaagtcgttt tgatctcaaa aggctcaccg atcaaaccca
      241 caacatcaga acaaagaatg tctttattca taatgctttc aacagttgtg gtcttctgaa
      301 gatttcccgt gccctccata gacacacgaa cctcggcaac agcgcaatct tcattttcta
      361 ccttatgaat aaatgtttgg aaacgctttt catcaccgtc aggggtaccg tttccgtctg
      421 tatcgggatt cagcggatcg gtttcaaagt agatctcgtc gccgtcgtaa agtccgtcat
      481 catcggtgtc aaccttaagc ggatcagtcc cgtaagtgtt gatctcttct ccgtctttaa
      541 gaccgtcgcc gtcggtatcc tcattgtaag gctctgtgcc atgctcgtac tcctgaagat
      601 ttgtcaaacc gtcttcatca aaatcctcat cgcagtcaag tacaccgtta tcatcagtat
      661 cgggttttgt ggaatctgtt ccaagagtta atgcctcata cccgtcggga agattatctc
      721 cgtccgtatc gggattgtaa ggatctgagc ctacttcctt ttcgataagg ttaggcagac
      781 cgtcatcgtc cgtatcggca agatactgcc agtcgtccat tgtccaactg agttcttcgg
      841 attctgtacc gacaagctct gcccacgagc tgctgtagtt aatattcgct ccgtatccgt
      901 cgataacaac attctgagca atgataagtc cgttcatatt gaagttgtcg cagtctatgg
      961 taacagttcc gaacggagca tagatcagtc cgtttaccga agcctgactg tttgagatgt
     1021 caatatcgcc gaactttgaa tagatgaccg tgttgttgcc gttaagattt ccgccggaaa
     1081 gatcaacgtc cgaaacagct cccacagcgt tattcaggct gatattgccg tctagactca
     1141 gtctacccgt aacgtagaca gaattattta tattaatatt catatcggaa tatgtataat
     1201 cttcgttgta tgtatcgcag ttttcggtaa aatatttgtc tgcaagcttg gtatgaatga
     1261 gtatcatatc cttgttgacg tcaaatacat cttcggtttc ttctccctcc gtattatctt
     1321 caatatcgtc ataatctgta acagtgccgt tgatattggg atactgagcg gttgtcgaaa
     1381 acacgccgtt tgtatatgca ttgccgttta aagtaaagct gtcggtgttt acgctaatgc
     1441 cggcagcttc gtcggcagca aaaatcgcat aaggatactt tgctgtttct tccgctgccg
     1501 caggcatgac cgcagctagg cttgcggtca tagctaaagc cagtgctcct gacagcgttc
     1561 gctttagccg tgactttgtt tttggtttga acatatttac tacctccata attatttatt
     1621 cttgttagtt tactaactcc ataaaaatac tacaaaatca tgctgttctc aacaaatttc
     1681 ttgtaaacac cccctttttc cttgtaaagg aacaaaaacg agatcagatt actaaaatcc
     1741 aacctcgttt tcgcatttca taaaatgttc tcattgatac caatatacac atcataaatt
     1801 ttgtttttaa tatcctgaac aatgaagtac ggaggggtag cgggcaaaaa agactggtct
     1861 tgaatatact tcttaagctc ctccactttg ttttgaatac tgtcgaaaga accgtaatgt
     1921 cttgtcctta tagcatttac aagtttgaac tccggtttat agcggtaatg cttgctgctt
     1981 gaaaattgtt tatctacagg aatcagaaac tcaagaaaag gggatttttg agtaataaca
     2041 attttcccgt cacgggatag atctaaaacg tcaagactat aatcgattct ttgggcaaag
     2101 tcctctattt ctccgtaatc aagctgcatt tcaaaacata aaacattttt ataatgtaga
     2161 tactggtgtt caactatcgg cataaccgca ccgccttact ttaagaattt ataaaatgca
     2221 agccaaaaat tatagaacat attatttgcc tcctttacaa atcgatgcca ttaagataac
     2281 accgatttct aaaacaggct acaaaattct tgtaaacacc cctttttttc ttgtaaagct
     2341 caaaatagct tgaaattaat gtcaaaatat tgtataataa gaacaacaaa aaatatcaga
     2401 aaataatagg gagaggttat tatgcatata gcagtttgcg atgatgagtc tttttttcga
     2461 aaaacgttgt ctgaagaatt atacatatat gcagataagc atggtcttaa ttttattata
     2521 tacgaatatt ccgacggcat ggaactctta gctgccgata tgtcctttga cttgattttt
     2581 atggatcatc aaatgacaga taaaaacgga ctcgataccg tttcggtatt gaggaaacgc
     2641 aatgttgata ctaccgtaat atttgtgagc agctataagg acgttgtttt tgacagtatg
     2701 aaagttaatg cttttcgctt tctcgttaaa ccgtttgaaa aagaggattt gtacgaagct
     2761 ttggattctg ttataaaaaa tctagcaaaa actgcttata tagtagttaa ggatactgtt
     2821 aatcataccc gaaaacgaaa taatatatgc acaagccgac aatatctatg cggaagttgt
     2881 cacagctcaa ggaatttttc tttattcaaa caaaatttct ttgttggtga aaaaattgaa
     2941 tgaagagtat ttctaccgct caaatcgctc gtatatcgtc aatttcaatt acataatcgg
     3001 ctacagtaaa aaggaaattg aattttctaa cggtcaaaaa gcgcttattt caagctctaa
     3061 attcaaggac tttaaaagca aatatctgaa ttatcttaaa agaaaaagta taggagaata
     3121 gaaatgaatt tagtacacac atttgaattt acagtaatat ctatactata tttatttacg
     3181 attatccaaa ccatgagtct tatttatgga accaaagagt acaggagaaa atatgtgact
     3241 gtatttctta tgtatttgat ttccggaatt atttgctcct ggtatagaaa tcatgacaaa
     3301 cctgttattt tcctaacggc aacaatatgt tattatgcat ttctactaat ttgcattttg
     3361 tcaatattca aatcaaaaaa attatggtct gtatatattt cggttattgt actgatttta
     3421 gattctattt tccaatcaat tggatgcata ctgattgatt tatttgtaca agattttgac
     3481 agagcaacgg ttttaaattt ttcatctatg atttttaata ttgttgtatt aatcctactc
     3541 agatttcttc aaaaaaatca tcaaaatcaa atcaggaaca gcataagatt actgccaaaa
     3601 cgattatata tattgatatt aattgtcctg gtttttatcg gagagctttg tggaaacatg
     3661 gctgttgaat ctagcgagtt gttctttgat aataaaataa acagtttctt gacagtgctg
     3721 accattatgg cttttttgac aattattatt tcatttgtat tcagcagtat ttcacggcaa
     3781 tactacgaaa gcatattagg aataatggaa aaacagatgg taggtcaagt tgagcattac
     3841 caaaagatca ataagcttac agacgatttg agagaatttc gtcatgatta taaaaatcat
     3901 atgatatgtt tgcaggcaac gcttgagaaa agacagcttg atgaagcact tgactatgta
     3961 aaaagtataa ccaagcagaa gataattgat gcaaataagt tttcaagcgg aaatcagatc
     4021 gctgattcga tactttcgga gaaaagcgaa tcagccgcaa atatcggcac tcagattcaa
     4081 ttcagcggtt ttatttccga tgagattcct gcagtcgatc tatgtacaat gctttccaac
     4141 gctttggata atgctatcga ggcttgtgaa aaactcagct taaccgattc ggctgttata
     4201 acggtgaaat gtgctgttgt acagaatgtt caggtgatta aattatcaaa tccaaatcca
     4261 gacaacaaaa attccacaga aacctctaag ctcgacaaag ataatcacgg ctttgggtta
     4321 tacaacattc gcaggactgt cgaaaagctt ggcggagaga tgagaatacc aaagcagttc
     4381 ccggagtttg ttctggaatt ggagtttcca atcaaatgat aaaagctgtt gtatggttat
     4441 ggtgacatac aacagctttg taatagctcc tattcggttc atttttattt ttcttcgtta
     4501 atggtataac gcaagtgcat actcggcaag atatttgtat tgtccgtttt tgaatgttgc
     4561 atagatttgc gaggtcagct tatcgggacg gcattttaaa taccccagtt cgcttacttc
     4621 tttcagatat tcttccttcc gcttgtgagg aacagcaggt aaagctttga ccccgttctt
     4681 tgctttcagc acctcgttcc agtccttaaa cttcggtgca agccgtttca catcggtata
     4741 tccattctct gaaagaatat cttttaagcg gtcaacggcc tcgatccctc cttcgtcatt
     4801 atcaacgcag agaattactt ccgataagtt agaatgattc tttaatgccg caagcacagc
     4861 attttcatac actccgttca ttgcaatgca gctgtgcttt tgccagtctt tcggataaag
     4921 cgtaagaaaa ctcatcatat ctatgggagc ctcaaacaca tagagccttt tgctttcacc
     4981 gaaatgtgca aagctgtatc ttgtgtcaga accttgacaa gtgatcctga aactattgcc
     5041 gtaagaattt gttgaacgct tgcttgcctg ccttggaacg ccgttttcat caattcctac
     5101 aaacacggca ttgtgatgtt ccttgtcctc ataaagcgtg tgctgttttg caaaatggct
     5161 gataatatcg ggagaaatga acctctgctt tatcaggtag gcataaaccc tatgcatatt
     5221 tgtgtttgtc tcgggaagtc tgaactcttt cttttcttcc tttgccgata ctttcggcgg
     5281 actgtgggac agcggagtta ccctctgtcc caacagttcc tgtaccgcag tctgaaagtc
     5341 cataccataa aactcctgca taaactttat tgctccgccg ccaacctgat ttttatggtc
     5401 aaaccatgtc gaaccacgga gcgttatgct gtcgtgtttg cctgaactgt cgtaataaat
     5461 gagcttatgc tct
//
LOCUS       DAWEMR010000174         5468 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5755_length_5468_cov_10.078884, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000174 DAWEMR010000000
VERSION     DAWEMR010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5755_length_5468_cov_10.078884"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1764..1773
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1518 a         1243 c         1083 g         1614 t
ORIGIN      
        1 gccaataaat gcggttttcg atccaattga aaagatcctc cgcagagtca ggaccccact
       61 cttcgccttt tctaaggtcg gcgtcatact ctttgagata ttcacgttct gtttttatac
      121 agccctgtaa aagcttttcc cggctcatct ctgtccactt ttccttggca gtagccataa
      181 accaatcctg cttgcaaaga acgataaacc atgggtttga acggtcgcct tttttagcca
      241 caaaattctt atctgtgaaa gcaccagcat aatatttctc agtgctgtca ttatatgccc
      301 agttaaaatc ccaaggagaa gtaaatttca gcttaggaca tttactgtcc tttgaaaaat
      361 cgacgcacat atagaagctt ccctcaccac agtcatagtc atgtacgatc tcataaagaa
      421 tatacatatc tctcactgag tctatatcca taacgttaga aacggcttcc tcagcggtgg
      481 taacatcgga gtcaacaagg tcataatttt catcaaattt ataatacttg ccgttctcgc
      541 aggcttcata aactatcttg aacagattgt tgagatactt gtcaataaag tctatctgat
      601 tttgcgaata gaggtcattc ttgatagaat attccgcagg aacgaactgc cttgtttcgc
      661 cgtttatatc ggtgacagtg gcattttcat agtccatgct tatataatgg ttatcctcat
      721 ctcttgttgc atagttgtca agctcaagat aatagcctat atcagtgtcg gtgtagcccg
      781 cctcaggctc ggagatatcc actctgtttg gatttatctg acactgttcg caaagctcat
      841 aaacgccttg aaactcctcg ttcacataga ggtgaacaag ctgaccgtct gagcagaaat
      901 tgctgtcacc catgatagtt ctgccaaaac ggaatgcgat atcgtttctt attagatccc
      961 aatctgattt caacagcacc cagcttttgc actctgcgcc gttattaagc ccaagcatat
     1021 ttgtcttttt atcaaatttt atcctataag gcactttgtt tgcaaggatc tgactaacat
     1081 caccgtaata tgcgcttgaa tttcctctta cctttatgcc tgctgaagct tcgtttatct
     1141 ccaatttttc atcacaattg aaaacgtcca ctacgcagga agtatacttc tcccttgaag
     1201 ctatcatatc gtcaggggca gtcgtaacgc ttataacagg tatcttactt tctgatcgta
     1261 tctttttctt aaaactttct ttttcagcct tgtcagcact caggctttca taagtagcag
     1321 gttcagagtt tatcacctgt tttgtggtga caggaagagt tacaacaggc tcagccgttg
     1381 ttgtcacagc ctgagattcg gcaggactac tctctgcttt tgagctttcc cctgtttctt
     1441 tagcacagct tgtgagcatt gctcccgtca tgactaccgc acatacagca gcgataattc
     1501 ttctgttcat atcatttctc atattattat ctttccattt attgtgcatt acacacatat
     1561 tttttatcat ataacttatt caattatagc acattgacat agataataca atatgctata
     1621 atcataaaag taagtgggat aatttatgca ctaatacgat tatataggag gttttttgaa
     1681 atgtttatac ctgacgataa aagatatgac aaaatgatat acaaccgctg tggaaaaagc
     1741 gggcttaagc tttgctgttt ccannnnnnn nnnatggaaa gaatggtaca cactgcgttc
     1801 aacagcggta tcacacattt tgaccttgca aacaactatg gcaatcccta caacggctcg
     1861 gcagaggaaa acttcggcag gatactcgac cgtggcatga gagaattccg tgacgagatg
     1921 tgcatttcca ccaaggcagg ctatgaaatg tgggcaggtc catacggtga cagaaacggc
     1981 tcgagaaaat atctcacggc aagccttgac caatctctca aaagaatggg acttgaatat
     2041 gttgacattt tctatcacca tgtaagagat cccgaaacgc ctcttgaaga gactgccctt
     2101 gcacttgaca atgcagtaag gcagggcaag gcgctttatg ttggcatatc aaattacaac
     2161 agagccgaca cagccgaaat gctgaaaatt ttccgagagc taagaactcc ttttattatc
     2221 aatcagccct catattccat actcaaccgc tggatagagg acgataaact tgacagcctt
     2281 gcactttctg agggctttgg cattgcagtg ttctcgccac tgtatcaggg ctttctcaca
     2341 agcaaatatc taaaaggcat accggcagat tcaagggtag gcagaggcga aacatggata
     2401 ggcaatgagc ttaacgacaa aatgatagaa aaactcaaca agcttaatga tatcgccgct
     2461 tcgaggggac agaccctttc gcaaatggca ctttcatggg tgcttcacaa caaggctgtt
     2521 actactgtgc ttatcggtgc aagccggcct gagcagataa cggaaaacat aggctgtctt
     2581 gaccgccttg acttcacaga agctgaactt gcagagatag acaggcttgc gaagatgtaa
     2641 taaagaagct gtcagagcca ccgccctgac agcttttttt atgcgttata atagcgaacg
     2701 taatcgtcaa gcatatctgc cgtaagctca aaaggataat ttttaagctt ggcagggttg
     2761 gcaagtattc cgtctctgtc cttcttccaa agctcctctg acatatcaag cttgccaaaa
     2821 agcgtcctga tatagtccct gaaagcgtcg atatcgtcaa agccaagaag ctgttttaca
     2881 acatactcca cctcggtgct gtcaccgtat tttttcaaaa agcctgacag aaaaacgcct
     2941 actgcattgc cgtggggtac gccattttca tatgtcaaag tataactcag accgtgaggt
     3001 atgcttgtgc ctgtgtaggc tatcgccatg cctgcaagca ctgatgcctg catgaaagtt
     3061 gagaactcat catcggtcat ttgagcaaat ctctcctctg tttcaagaca gcccttacag
     3121 cttgcccaaa gcctcatacc ctcctctgca tatgcacggc tgaacgcatt tgcgttggtg
     3181 ttgagccgtg actctatcat gtgagcaagt gcgtcaacgc aggtgctttt cataccgcca
     3241 tatgatgatg ttttaagata gccgtagtcc accaatgcaa gtgacggaaa aattttgtgg
     3301 gcaatagatt tcttggtatg cttgatgtgg gaagtcagga tagaataagg tgtagcctcc
     3361 gagcctgttc cgcaggtggt aggcacacag actatagggt aaaaaggtag tttcgcagga
     3421 gtgtacaaaa gctcctccac cttggcaatg ctgtctggaa gctcacactt ctcagctttt
     3481 acaacgccct cgttttcgga aaactcggca ttttcgcaaa ctatcccatt cttagcaagc
     3541 attgatatcg ccttgcttgc gtccattggc gaacctccgc ccactgccac gaacatatcc
     3601 acgccgtatt ttaccgccgc cgttgccgct gtgtaaacag tttccacaga tgggttttcc
     3661 tcaatatcat tatatatcac atatccaatg ccctgacttt caagcactgc cctgacatca
     3721 tcaagagaac cattcaccgc agaagaatgt ctgcctgtga ctatcatggt ttttttgcca
     3781 agttccgcta tctcgccccc gtgatttttt accgctccat tttctatgta aatatctgta
     3841 ggtatatttc tcattttcaa cgctccttct ggctttttat ccattatagc acctttgacg
     3901 gcgattttca agccttgact ttgtggaaca tatgtgctat aatattagta ataaatcatg
     3961 gagggaaaag ctatgcactt cacaacagca aaaggtatac tctcgcaaaa aaacggcatg
     4021 aatttatatc gtggctgcac ataacggtca gacgagtatt tttgatactg ccgattgaaa
     4081 acctctccca tttatgttat aataaagaaa aaacatcaag gtgaagcaat gaataaaaat
     4141 caaaatatag ctatggggat atcccttgcg gttgtggcat taatggtcgg ctcggcggag
     4201 atcttcgggg aaaaggaaat aatttttcct gagataacgg cagtggcaat gggtgcgcta
     4261 attgcgcctg tgcaatcgtg gaacacctct cgaacacggc ttttggcggc gatagtttct
     4321 gccgccgtcg caggcgtgtg catagtgcgg tttatccctg agatacttat acttcgtata
     4381 gctttggggc ttcttgtagc agtgagcgtt ataacactgt caaaaactag cttcgttcct
     4441 gccgtttcag cctgcatact tccgatactt atgggtacaa aaagccctgt ttacgtcatt
     4501 tccgttgcgg tcatgacagc atttatactt atttttcaaa aagttcttga gtgctttgaa
     4561 cttcatgaaa agtatgaata tagacctatc gaaccaagct cagagcttct caaactaaga
     4621 gcaaaacaag tcatcacagc gcttgttatc tgcatattgc ctgcgcattt tcacgagata
     4681 ttctttatcg ctccgccgct tatagttgca ttttttgaac ttagcggcaa aggctcaaag
     4741 cttatgaaaa gaaaatacac cgcaattgct ctcatggcgg tggctgcgtt tgcaggcgtt
     4801 atggcaagat tttttatctc tgaaaagctt gggcttaccc tgacatttgg tgcagtttta
     4861 tcctcggctg tgatattcat cctttgccga aaagcaaagc tttattttcc tccatgctgt
     4921 gcaatatcta ccttgccttt tataatccca aaaactgcaa taataaaatt tccatttgag
     4981 gtcacagcag gatatattgt ccttaccatt gccgcagtta tcataaccaa aatagacaat
     5041 aatacaaatt aggcataatc aaaaacgtac aaagctttat gatccgaccc ccagaagtta
     5101 gaccgaaaat ctaacgatct ggaggtcaca tcattacgct ctgtacgttt ttttgtatat
     5161 taagaaatca ttctctgttt ttaagcacct cggcaggggt tatcttgcca agctttctta
     5221 caagaattga ggtgatgata aagtatgttg ccattactcc tgcaaaaatt ataacgtaag
     5281 cataccatgg gaatgcaagg tttattccgc ttgttacgtt tggaatgaac actgggaaaa
     5341 ccttgtccat aaacatcttt gaaagcggta tctcgataag tgcgcccaaa gttatgataa
     5401 ttgcgtttcc gtttaggtaa agctttttca cgtccttggt cttatagcca aacaccttca
     5461 caagggat
//
LOCUS       DAWEMR010000175         5430 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5793_length_5430_cov_13.452279, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000175 DAWEMR010000000
VERSION     DAWEMR010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5793_length_5430_cov_13.452279"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1403 a         1320 c         1097 g         1610 t
ORIGIN      
        1 cccttttaag ggtggttgca gtcttttttt attgcgcgtt tttttacagc gcctttttct
       61 attttatcag atagccgcaa agccctgctt cagatcgtct atgatatcct cgacattttc
      121 aagacctacg gaaagtctta tcattccgcc gtttatgcct gcggcaacga gctgttcgtc
      181 tgaaagctgt ctgtgtgttg cggaggctgg gtggaggata caagtacgga tatcggcaac
      241 gtgaacttcg ttggaagcaa gcttaaggct gtccatgaac tttgccgctg tatctcttcc
      301 gcccttgata acgaacgaga taacacctgc tgtgccaaga gggagatact tttcagcaag
      361 cttatggtac ttatcgcttt caagtccagg atatgcaacg gactctacct tgtcattgct
      421 ttcgagaaat tttgccactg tgagcgcatt ctcacaatac tgcttcattc ttacagggag
      481 agtttcaagt cccagattga gcaggaacga ggaatttgcg gcaggatagc agccgaaatc
      541 tctcataagc tgcattcttg cttttacgat ataaggtgca aaagaatatt tctcagtgta
      601 gatagtgcca tgatagcttt cgtcaggctc ggtcatgcaa gggaactttc cgcccgtcca
      661 atcgaacttg ccgctgtcaa cgataacgcc gccgacctga acagcgtggc cgtccatata
      721 ctttgatgta gaatggataa cgatatctgc gccccactct ataggtctgc aaaggtatgg
      781 agtagcgaaa gtgttatcaa cgataagagg aacgccattc ttatgggcga tatttgcaaa
      841 acgctcgata tcgaaaactg tcagcgcagg gtttgcgata gtttcaccga aaacaagctt
      901 tgtattatcc ttgaatgccg cctgtatctc atcatctgta gcgtctgggt caacaaagat
      961 acactctatg ccaagtcttt tcattgttat gctgaaaagg tttatggtgc cgccatagat
     1021 agaagtggaa gctataaagc tgtcccctgc ctgacagatg ttcaggatag agcaaagtgt
     1081 ggcagcctgt ccgcttgatg tacacattgc ggctgcaccg ccctcgagag cggcaatttt
     1141 cttttcaact gcgtctgtgg tagggttttc aaagcgtgag tagataaggc tcttggtagg
     1201 gtcgtcaaag acctcggcta tctctcttga ggagtcatag acataggttg tactttgtac
     1261 gataggcact actctcggct ctccgttttt aggcgtataa ccctcgtgta aacattgtgt
     1321 ttcgatcttc aatcaaatca aatcctttca atgcggtaac gaccgcaaaa tttttaccaa
     1381 tataaaaagc ttttcaaaaa gcgtttaaaa cagcagtccc ctcagaccgc ccaaacatta
     1441 taaatgcgtt tattctgcat agcgaaaact catagctgaa tcgaaaatat cttctcccat
     1501 tgaaacatac tttagtggta tcaccacatt atattgtata tattcgctgt cagaaaactc
     1561 gtatatgccc tgaacgttca cactttgatc ctcacccaga gccgcaataa cacagccacg
     1621 cttgccattg ccaaggtcaa gtccccgata atcaagccaa acattatcat cagagctgat
     1681 atcagccgac tttgcataca tatctagcat atcatcgacc gtggtcttgt caagccgctg
     1741 ataataagac aatgacagat ttaacccgtc atatttttcg tccactttgc cgtcaataag
     1801 atttgcatag acaatcttca catctctcgt gccgtcaaaa tcatctgtag ttatctcatg
     1861 cttttccaac tttgcggcag aaaatccacc tatctcaggc aactctaaag cggcagaaaa
     1921 atccgaaagt tctatggtca aaccgttttc aatatcataa cggtctatat ccaccgactc
     1981 tccgttaaac atatcataaa catagctgtc tatgaccatg taaacgccac ccgtggtgat
     2041 attcgcagaa atgccatttt cgtcaacgaa actttcaagt ttcgtgaacg tgctatcctc
     2101 gtcaaactgc attataactg cactggcttt ctgctcgtcg cttattttgt cgctaaactc
     2161 aaaacgaata accccacccc cgaaatcagc gtcagtatac ttgacaggta tactaaggag
     2221 cggctcgtca gaatttatca ttgcgtttat accataataa tcagaaagct ttgctcctgc
     2281 accaaaattg ccctccacgg tgcatttggc tgtatatggt atttcgctaa ggtcacaaac
     2341 aagctcaggg tcacgaatta tttccacctg cgaactgtca tccgtctgcg aactttcctc
     2401 cacactgtcg ggcgtatctg acaccgcttc cgcagtagta gtatctttcg cttctgaaac
     2461 gctgtctgcg gcttcctcaa caggtccgca ggcgcaaagt gacactgaca gcaatgccgc
     2521 cagcaatacg gctgtttttc tgctattttt catattaagg ttctccctat ttttttattt
     2581 tgtttatctt acaagtcctt gaagtccacc cttgcccccg taagagcact ctccattact
     2641 atccttgcac attctgaaag tgaaactgcg cttttgcagg tcatcatggc tttttgtatg
     2701 caaagtccga aaactatgtg aagctctccc ctgcctgctg acttctgcgc tataggcaga
     2761 agcggttgcc aaagaagctt tatatagctt tggacagttt cgcctgtggt acagtttctt
     2821 attttgccaa agcttgcgcc ccctgcgttt gccgcagtgt agcggagcat ttccccaacg
     2881 tccgtaaaag gctcgttata tttttgaagc actcctgcgg taaaagacca cactgagtag
     2941 ttattgccca caagcttttc gtcaatgata tcaccgtaga aatactttct gcccaactta
     3001 tcagtgaaca ctgtgaaaac gtcctcttct ttcacaccct ctgtcatgaa aagctttctc
     3061 acgtcctgct gacaggaata gcctgcaaga gaacctatac agcacaaaag ggattgcggg
     3121 tggacatagc cctcggggct tctcatggct ctgagaagtc tgtcgaatat ttcaaaggca
     3181 ctgcttttat attgttcaag ttccattttt atcaccgttt ctcacgtttt cgctcccctt
     3241 tttatctttt ttatcacaaa tcttatgata ttaacataga tcaccgcaag cccaaagcca
     3301 ccaagcagac ctgttaccag ccgacgttta ttggtggact ttttcacccc tgtatactgc
     3361 aatgaccaat ccagcatggt agtttcacag cagagtacgg ccgtgagggt ggatatgttt
     3421 ttctttatgg ctatcggaaa gcctataagc tgtcctgcaa gcacgcctgt acatcttgcg
     3481 cacacaggaa actgccagcc gtgaaaaaag aagcttctgt cggggcgttg gtgacagcct
     3541 gccgacgcac caagcttcat aagataaagc cacagtgcat atgggtcttt tctcattatc
     3601 atgctatgct ctgaacttat aatcgcaatc aggacagacc caataggagc gtgtttttga
     3661 cttgctttcc atgccgcaaa gtccgcagat aagccctggc agtccgaaca gtatatagcc
     3721 gcagcagcct tttccaaagc cgtatttgcc gcttctgtag tctgtttcgc ttataagctg
     3781 acagttcctg ccgccacatt ttggacagcg tttgcctgta caaaaatctg ccatggtgtt
     3841 ttccgccttt cttttgtgtg gtgtttatca gataaaattc aggggcttga cgatctgaaa
     3901 agacctatca gcccgctgaa aaaaattatt ttgatattat acgtttattt ctgcaacatc
     3961 gtcagacaca ccgtttgctt cgacaacagg ctttacgcaa ttttcgataa actcgtccac
     4021 ctgctgtggg catctgccga taaagttttc aggcttcatt atcttatcca tttcttcctt
     4081 tgttatcatg aacatagggt cgttgagtat aagctcgcag aggttattgt caagtccctc
     4141 tttcttaact cttgcgcctg ctaccattga atattctctt attctttcgt ggagttcctg
     4201 tctgtttccg cccttttcta cagcgtccat cataatgttc tcggtagcca tgaaaggaag
     4261 ctctgccata actcttcttc tgatgatctt gtcatatact accaagccgt tgtcaggatc
     4321 tgtaacattg agcatgatat tgagtatagc gtcaacacca aggaaagctt cggcaacgga
     4381 aattctcttg tttgcagagt cgtcgagagt tctctcaaac cactgtgtac ctgctgtgaa
     4441 tgcagggttg agaacgtcgc agataacgta tctggcaagt gatgttattc tctcatctct
     4501 cattggattt ctcttatatg ccatagcgga agagcctatc tgatttttct cgaacggctc
     4561 ttcgatctcc ttgaatgact gcaaaattct tatatcgttt gagaacttca tagcagactg
     4621 agcgataccg gcgagaactg agcatacctg atagtccatt tttcttgaat aggtctgacc
     4681 tgaaactgga acgacagcgt caaagcccat ttcctcggct atcatttttt caagctcttt
     4741 taccttatca gtatcaccgt ggaaaagctc cataaatgat gcctgagtgc ctgtagttcc
     4801 ctttgagccg aggagcttga ggttagcaag tctgaaatcc agatcttcaa gatccataag
     4861 aagctcgttg caccaaagag tagctctttt gcccactgtt gtgagctgtg caggctggag
     4921 gtgagtataa gcaaggcaag gcatagcctt atacttatcg gcaaactttg caagctgatc
     4981 gataaccttg acaagctttc tctttacgac cttaagtgca tctctcatga tgataacgtc
     5041 tgtgttatcg cctacatagc atgaagttgc gccaaggtga atgatacctg ctgcctttgg
     5101 acaaacaagg ccataagcat aaacgtgtga cataacgtca tgacgaacaa gcttttcacg
     5161 ctcagcggca gcggcataat ctatattatc aatgttagct tcaagctcgt ctacctgctc
     5221 ctgagtaaca ttaagaccga gcttcatttc agcacgggca agggctaccc acagctttct
     5281 ccaagtagta aacttcttgt cagcagagaa aataaactgc atttccttgc ttgcatatct
     5341 ggtacaaaac gggctttcat aagaatttgt cattgttctt cgacctcttt cgtaatatta
     5401 tataatgtat acacacctat tatagcatat
//
LOCUS       DAWEMR010000176         5427 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5798_length_5427_cov_8.571482, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000176 DAWEMR010000000
VERSION     DAWEMR010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5798_length_5427_cov_8.571482"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1696 a          974 c         1225 g         1532 t
ORIGIN      
        1 ggctcgaacc actagatttt aagggaaaag gtgcgttcca ccatttcctc gtacaatagg
       61 ggttgagtat gtttttcgtt gaacaaccga gccacgtgga gtgtgtggta ttgataacaa
      121 ggaaagcgca gtaaatcagg acagttagct tagcggtgtg gtttacacac attggttaaa
      181 catgccgctt tttatagggc atggcagttt agcgtatagg ataatatgtt tgtggtgaca
      241 acagttccac agcgatacaa gtaacgttgg agatattatg gatagaactg taagaaatgc
      301 agcaaaagca ttaattatta aagatgataa gatgttggca atcaaaataa gtgacggaaa
      361 agaagaatgg tatattatgc ccggcggcgg acaagatgtg gaggaacttc ttcctgaagc
      421 agtttgtaga gaagtggcag aagaaatggg attgcgggta gttgtgaaag atttagcgtt
      481 tgttatagag ggtttacatg gagaaaaatt tcatcgtgta gatttggtgt ttttatgtga
      541 gtatattgga aaacttgaaa atgccgtctt gcaaagtgat acaaatcaag ttggatatga
      601 ttggttagat attaaaatat taaatacaac accattatat ccttcaaaat tacgccgaca
      661 gatcatgaat ttgtatgaag gaaaagcgta taaagtttat ttaggaaatg aagaaattgg
      721 agacccggaa gtaacagact aaataatgaa ggaacttgtt tatgagaaga gtgataccag
      781 caaataaagt aaatcatgat tatgagaaag tattgagtga atttgtgtca gccttaaaaa
      841 atagattagg tgaatactta ttaatggttt atttgactgg aagttatgcg agaggagatg
      901 caaatgataa ttctgattta gacgtgtttt gtatctttag tacaataaat caatatgtct
      961 tagaaaatgt tgggcattgt gcaagaaaca catctatccc ttatgatttg ttggagataa
     1021 atactcaatg cttatccgta gacgaatata aaagcaaaat atttgaagat tggtcagttt
     1081 tattgtatgg tgagcaattg gtgaaaatcg aaaatataaa tgaaaagata cagttatcct
     1141 ataagaaaaa tttagcaaat attttaatga gtgttcgcca ttatatatgt gtagatgagc
     1201 caaaagaatt acttacgcac caaaaaattt cgacatatat tttgaagccg ctcatgtttg
     1261 cattaagaca agaacgcttt tgtgcaacag gagtttatcc cttgtcgata gaaagtttat
     1321 tagaatcgta tcaggatgat aatagaatta tggttgaata ctttctaaat aaggaaagat
     1381 ttgaaacgga cattttgtct gatcataagg atgttctgat gtttcttaat gataaaatac
     1441 aaaactttct tgaaaatgat ttataattag atagcatgaa gaaagcatat atacgaaccc
     1501 tttatatcgc agaaatgggc atgtctttat atgaaaagtt tggatttgtg caaatgaaga
     1561 atgaaatgga gtacattgaa atttagcaaa taggaagaaa atatgaaaaa aacagtaata
     1621 ggcataattg cccacgtcga ctcaggcaag acaacgcttt cggaagcaat gctttatttg
     1681 tctggcaaaa taagaaaggt cggacgtgta gaccacggtg acgcttttct tgacacggat
     1741 atgattgaaa aatacagagg tataactgtt ttctcaaaac ccgcagaatt tgaatataaa
     1801 aacacatctt tcaccttgct tgacacacct ggtcatgtcg atttctcagc ggaaactgag
     1861 cgtgcattac aggtgcttga tcttgctgtg cttgttataa gtggcacaga cggtgtgcag
     1921 agccataccc aaactctttg gaggctgtta aagcgttata acgtcccagc ctttgttttt
     1981 gtgaacaaaa tggacttgga cggtgctgat aagacttttg ttatgaacag cctagagggt
     2041 cgtctcggca gtggatttgt ggatttctgc cgtatgaagt ccgaagtcgc agaggattgc
     2101 gccctttttg atgaaaatat tatgaatgag tttcttgaaa cgggatcaat gtctgacaag
     2161 atactgtcag attccgtggc tcatagaaaa gtatttccat gctttttcgg ctcagcttta
     2221 aaatcacagg gcatagaaaa atttatgaac gcccttgcag agcttgcacc gtgtgtatat
     2281 gacagcggta cttttggtgc aaaagtgtat aaaatttcag aggacggtgg ctcacgcctg
     2341 acttttatga agataacagg tggctgtctt aaggttagag atctagtaga ttacacatcg
     2401 gctgacggtg aaagcttctc agaaaaggtc agcaggataa gaatatattc aggggcaaaa
     2461 tatcgtaatg cggataacgc tgtaactggc gttgtttgcg ctgttgaagg gttgactaaa
     2521 acttatgcag ggcagggatt tggttttgag caggattctg caaaaccact gctggagcct
     2581 gttcttacct atcgtgtcga gcctgtgtgt gatttggata tgcacactct gctttcatat
     2641 ttccgtgtgc ttgaaaatga agatccacag cttcacgtcg attggaatga acagcttggc
     2701 gagattcatg tcagtataat gggtgaggtg cagcttgaca tactgaaaag cattttcaag
     2761 cgacgctttg atatcgacat tgattttggc gagggaagca ttgcatacaa agaaactatt
     2821 gagaaaacgg tttacggcta tggtcactat gagccgctca ggcattatgc tgaggtgcat
     2881 ttgaagcttg agccgctgga gcgtggcaag ggtctgcgtt ttgcaaccga atgcagtgaa
     2941 gatacgctgg acaaaaactg gcaaagactt attcttaccc atttgcagga gaaaaagtat
     3001 cttggcgttc tcacaggctc tcctattaca gatatgaaaa taacgctcgt ttcgggaaga
     3061 gcgcacctta aacacacaga gggcggcgac ttccgtcaag caacatatcg tgccgtcaga
     3121 cagggactta gaaacgcaaa gtccgttctt cttgagcctt attacagctt cacacttgaa
     3181 gttccacagc agaacgtggg tcgtgctata acagatatac agaacatggg cggagttttc
     3241 tcacagccgg aggtatcggg cgaattttcc gttataaagg gaagtgcacc ggtggcggaa
     3301 atgcgaggct atcagtcgca ggtcataagc tacaccaagg gcgttggaaa gctgatatgc
     3361 acttctgacg gttaccgtga atgtcataat acagaagttg tgcttgaaga atatggctat
     3421 gatcctgata gagatcttga aaatacggca gattctgtat tttgctctca tggcgcaggt
     3481 tacaatgtca aatggaacga agttcctgat aagcttcaca tacctccaga ggacaaacgc
     3541 cggcaggtct ctcagccaca gacatatgct cgtgctgaag attttgtaag acgtgcagca
     3601 agtgataagg agcttatgga gatatttgag cgcacttacg gaaagataga acgtgataag
     3661 tactatgcga tgcgcagacc tgaaaagtct gtaaagtcag cgtcaaagcc gaagcagata
     3721 tactcaggcg tggaatattt gctggttgac ggctataaca taattttctc ttgggacgag
     3781 ctgaaaaagg ctgccaacga gagccttgat cttgcacgaa gtatgcttgt gaacaggctt
     3841 tgcaactatc agggatacaa gcaatgtgag ctgatactcg tctttgacgc atacaaggtc
     3901 aaagagcagg agcgtgtagt ggaaaattac cacaatatca gtatagttta caccaaagaa
     3961 gccgaaacgg ctgatactta tatcgaacgc actgctcaca agctcagccg agagcataag
     4021 gtaagggtcg ccacatctga cggcatggag caggtcataa tcatgggcag cggcgctttc
     4081 agaatgtcag ctcaggagct tcacgaggac attatccgtg tggaaaaaga gataagagaa
     4141 tatatctcca atatgaaata acaaaaaacg attttctgaa aataaacaga aaatcgtttt
     4201 ttgttatctc ttccttttct tgggctttat ttttatctca ggtattgcta agttgtcgaa
     4261 tattacgcct gattcgctat tattttcgtt ttcatcattc tcagttttta catcttcaac
     4321 gtgaacttct ggaaaagcaa ggttatctgt ataacagtca agatgtttgt tctttttttt
     4381 catattcttt tcctccgtta attaataaag ttgataaatc ctgttataac aaggctgttt
     4441 ataaaatcgg caaacatact tccaacaata ggaacaagca tataagcctt tattgaaggg
     4501 gcaaattttg ctgttaaagc ctgcatatta gccatagcgt ttggcgttgc acccattccg
     4561 aagccgcaag ttcctgccgc aagcactgcc gcatcatagt ttctgcccat tatattatat
     4621 acgataaagt atgagaacat tgccatgaga agcacctgtg caacaagcat gattatcata
     4681 ggcaatgcaa gctcggcaag ctgccagagt ttcaatgtta tcatggctat tccgagaaaa
     4741 agtgaaagac aaataccacc gatatcattt atttcaccca tgtgtatgtg tattttaccg
     4801 ctgtattcac cgatatttct cataaatgct gcaacgatca ttgatccgat atacacagga
     4861 aaagtcatgc ctgaatatga aagcacccat gatacaaacg tgcctattcc cattgctatc
     4921 gcaagctgat aagctgcagg tgcatacatg cttacagaac ggtgatgttt ttgttcgtct
     4981 tcgacaagta tagagtcgtc ctcaggaata acagttttca gcagatcgtg tttgagtata
     5041 agctttctgc cgataggtcc gcccattaca gagcctataa tcaatccaaa agtagccgct
     5101 gctgtgcaga ggcttgttgc tccttgctga ccaaggtcct caagcactgt gccaaaagca
     5161 cctgacgttc cgtgaccgcc taccataggg atagatcctg tacacatacc taccagcgag
     5221 cttactccca atagctttga aagtcccact gcaaccgcat tctgcgcaaa tatcagcact
     5281 atcaggcata ataaaaatac aataagactt ttacctccag ctttcaaaac tttaatgttt
     5341 gcctgaaagc caacagaagt gaagaacatg accatgcaaa ccgtttttag cgtttcatca
     5401 aactgaaatt cgactattcc tgcgacg
//
LOCUS       DAWEMR010000177         5370 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5861_length_5370_cov_14.875447, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000177 DAWEMR010000000
VERSION     DAWEMR010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5861_length_5370_cov_14.875447"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1505 a          892 c         1461 g         1512 t
ORIGIN      
        1 aattgcagaa atgttgccaa attaaaatat aaatcatttg tttgcacaaa tttgtagagc
       61 aaattttgtg cagggcgatg ataaaatgtg agttatcaga aaataaaaaa tagatgcttt
      121 tggcataggg ggtatgatta tgaagaaatt tttggctata gcggctcatg ttatcagtgg
      181 cctgggaaat gaccttttgg gctgggtgat aatcattagc tttgagctga caggcagtga
      241 ggggaagttt cagtacggtg tttttcattg gataattttt gcttgtggtt tgatacatat
      301 tgcggtgagc gtgctgtaca gtttgttggt ttggaaaaag ggtacggcga atggtcatgc
      361 tctgtcggga aaaattcttg cagtttatga tattgttatg acgctggtac cttatgtgta
      421 ctggtttgtg gtttgtgtgc tgtaggtttg ggtaaggtga aactaacgga cgaggtttgc
      481 cgtatggctt tggataatat gtgataggtt ggaggttgag cgttatgaaa aaatttagga
      541 cggtggcggc tgttgttatc atgttttttg cggctatggc tggctatttt gttggggcag
      601 cattgcagga ggaaatgggc ggtgcgatac tgtttgcggt gataaccggg tttgcttgcg
      661 ttatttacaa tatggataat cgggaataga gttggcgttg tttaacggat agaaaggtga
      721 aaatatggaa aatacgttta gtgtgaagat gatatttcac ttttatcaca gtgtggtaaa
      781 ggcggagctt aaaaggagag gctttcctaa agaggtggct aagaagatag aggaagaaca
      841 tgggaagata gttgcgaggg ctaaggatat tggtaagtca aagcttttga gttcgtacat
      901 tatggcgagc tattacattg cgatgaacag gagcacaggg aagtctgcgg aggagaatta
      961 cgagatattc cgtgacgggc tttgcgcttc aaagcttttt cacaaggtca tgggtgatgt
     1021 ggacagttat cttgacccta agaagatgcc ggggcgtttg aaatggtcgg aagacagcca
     1081 caagaggtta tatgaaaacg attgggtggt ggatattctg ccgggcaatg gtgaatatga
     1141 ccttgggtat gattatcacg aatgtggggc gtgcaagctt tgtaaggacg aggggtgtcc
     1201 tgagctggcg gcttacatat gcagaatgga ttttgtgctg gcggacatga tgaacatgaa
     1261 actcgttagg acgggaacta ttgctgaggg tgcaccatac tgcgatttta ggtacagcag
     1321 gaagaagtga caggctcata ggggctgaga gttatttaag agaaggtaaa aaagaatgaa
     1381 agagttggag tatccatttg acaatgggtt tataatgaaa aagaaaaggt cgctgaaaag
     1441 gcagcttttg ggcgacggag cggtgcggct gaaaaagagg gtggctgtgc tgggcggttc
     1501 gactacggac gatatcgtgt cggtattgga gctgtttatg cttgacatgg gctttgaatg
     1561 tgagttctat caatcggaat acggacagtt ctggcaggac gctgtgttct caaacgagga
     1621 gcttgaccgc tttaaacctg atatcgttta tatacacacg agccttagaa acctcagctt
     1681 ttcacctagc cccaggtcgg gtgaagagga gatagagcag gggttaaatg ctgagcttga
     1741 caggctttct caggcttggg acggagtgaa agagcatttc ggctgtcctg tgatacagaa
     1801 caattttgag cttccgtttt tccgccttat gggaaacatg gacgcttctg acaggcgtgg
     1861 aaaggtgaac ttcgtcacaa ggctcaattc ggctttgtat gacaggatag cccggaggcc
     1921 ggaggtttat ctcaatgata taaactggct gtcagcggca tatgggcttg aaaagtggag
     1981 cgagccgaag tattggtatc tctacaaata tgccctaaat atcgaggcta tacctgagct
     2041 ggcttttcag gtggcgagta ttataaaggc tatctttggc aagaataaaa aggcgctggc
     2101 tcttgacctt gacaacactc tttggggcgg catcgttggt gacgacggtg tggaaaatct
     2161 cgagataggc aaggaaacgg ctgaggcaat ggcttatttt gagtttcagc agtatgttaa
     2221 ggcgcacaag gacttgggcg tgcttttgac ggtatgctcc aaaaacgagg aagagaacgc
     2281 tttggctggg ctttcccacc ctgagggcgt gctaaggcct gatgattttg tggcgataaa
     2341 agccaactgg ctgccaaagg acaaaaacat cgttgacacg gcggaggagc ttaacattct
     2401 ttctgaggcg tttgtttttg tggacgataa ccctgcggag agggagatag tcaggggaca
     2461 gcttggggga acggctgtgc ctgagatagg ggaggtcacg gactacataa gagtgcttga
     2521 ccgttcggga tattttgaaa ctgttacgct gtcggaggac gatctcaaac gcaatgatat
     2581 gtacagggcg aacgctcagc gtgcaaaggc gcagagcagg tttgctgatt atcatgatta
     2641 tcttttgagc cttgaaatga cggcagagat aggggatttt tcgcctttgt atctgcaaag
     2701 gataactcag ctcaccaaca agagcaatca gttcaatctc accacaaaga gatacactgc
     2761 ggagcaaatg gaggcggtct ataactctga tgaatacata aggctgtatg gaaagcttta
     2821 tgacaaattc ggtgacaacg gcgttgtggc ggtgactatc ggaaagacag acagcgagaa
     2881 caagggcagg cttgatatcg agctttggct gatgtcatgc cgtgtgctga aaagggatat
     2941 ggagcttgct atgctggata gtcttgtggc acaggcaaaa aagcgtggca taagggagat
     3001 atacgggcat tattacccta cggcgaaaaa taaaatggtg gcagggcttt atgaaagctt
     3061 cggctttgag aagatatctc aggacgaaga gggcaacacg gcttggcggc ttttagttga
     3121 ggggtatgag gataaaaata ctgttataaa ggtgaaggag gagcattatg cttagcaagg
     3181 acgagataat tgaaagactt gatgatgttt tcagagatgt ttttgatgac gatactttgg
     3241 aggtggacga gaacaccacg gctgatgata ttgaggattg ggactctatc gagcatatca
     3301 ctcttatcgg tgcggtggag gacgagttca agatgaggtt caaaatgggc gaagtttcag
     3361 gtatgaaaga tgtgggcgag atgatcggca ttattgagca gagaggcaag taacaggctg
     3421 tttgcgaaat atgcgtgaga acgctgttct ttttctgaat tttgtggttt ctagcggcgg
     3481 tatggctgaa aggctgtact gccgtaaact tttaatgtaa attcataggt tatacttgaa
     3541 atatctgcga aaatggttta tactattggt aataaaacta ttttacggag gatattatgg
     3601 aagataattt taccctctgt cagggcgtat ctgtgggcgg agcggaaaaa ctgcggtctt
     3661 gctgccacat tcagggggat aatataaggg gtgtgctgag ttgtgacatc atggcgaagg
     3721 tcgcctgcgg tgtgatagag aagctcgctg agcctatatt cttctttctg gagcttcctg
     3781 acagcgagag agatggatac aagacctatt atcttgataa ctgcacgaaa gaggtcgccc
     3841 ttgccataat gaagcgttac ggcactgtgc ttgtaaatga cggagtatcg agatttggat
     3901 ttggctcgca caagaatgac gaggagatat atttcacaga ctatcaggaa tttcagatat
     3961 actcacgcaa tccaaagaag cttgagaagc tttttgacag acttgggata gacgttgtgg
     4021 gcgaagatga agttgtaaca ctgtgggact tgataaacga ggacaacgcc ggttgtatct
     4081 catgcgttga ggcggacggc gaaacggttt ttgacatacc tgaaaatcta aagtctgagg
     4141 gtatgtacga agctgaatga aaatacagga ggaatgtaca tgaacatctg gcacgatatt
     4201 tcaccaaagg tgataacaaa ggaaaaattc acggcggtaa tagagatacc aaaaggctca
     4261 aaggtaaaat acgagcttga caagaccacg ggtcttctga gaatggacag gatactttac
     4321 acgtcgaccc actatcctgc aaactatggt tttatcccta ggacgtatgc agaggacggc
     4381 gacccattgg atgtacttgt gctttgctcg gaaacgcttg cgcctttgtc tttggttgac
     4441 tgttatccta taggcgttat ctccatgctt gacaatggtg cggctgacga gaagataatc
     4501 tgcattccgc tcaatgatcc tacatacaac atatataagg acatttcaga gttgcctaag
     4561 cacatttttg atgagatgtg tcatttcttc tcggtataca aggcacttga acacaaggac
     4621 actgttgtcg atgatgttaa ggatcgtgaa catgctactg agatcgtggc acagtgtatc
     4681 gacagataca ttgagaattt ttgcaagtaa ataactgagg cttggcggac gcaccccggt
     4741 gcgtccgctt ttttactgca tattgtattt ctacccttgc agttgccttt tttgccactt
     4801 tattttgcgg ttttgtgcaa ttttactatt atttttggct gttttaacgt taaaaaccct
     4861 ctctctcgct ccattcctct tcaaacttta ttttctttat ctaagcaatg aaaatttaca
     4921 aaatcggata gtgtaataat taaattagaa aacaaatttc cttgttaccg aattttaacc
     4981 tttttgcgac actatattct accgttcatc actcttcttg ctttttctgt cacagcgtca
     5041 gaaataattg tgcttttttt cgcaaaacct attgacgtat actatatgtt gtgttacaat
     5101 aactttataa ggtcaatacg atgtgaatgt atgggagttt cgcggaaata tctgctatct
     5161 ttatacaggt gcaaatgttt aaagtgccac aagatattgt atgacgtaaa attaattaaa
     5221 agtttacaaa ttacaagacg gtgacaaatt aaaaggtgaa agttacaaaa tccccctaaa
     5281 atctctctca aattgaccaa gtgtccaaga aaaatccctt gcaaaaattc actttgggaa
     5341 atattactgt tgcaattttc tataatatag
//
LOCUS       DAWEMR010000178         5317 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5930_length_5317_cov_10.702585, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000178 DAWEMR010000000
VERSION     DAWEMR010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5930_length_5317_cov_10.702585"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1669 a          984 c         1006 g         1658 t
ORIGIN      
        1 cctatctctc ttttcttttt tcttaatttt gtccaatatc tattgtagca ctacatttta
       61 acaagttttg cttctaaaaa tcgacttgct ttccccgcag aaaaatggta taataggaat
      121 aacaaatatt ttcaaggagg caagacaaat gggcatttac gttaaccctg ataatattga
      181 ttttcagcgt gcaataaatt cacagatata tgttgataaa agtggactta tagaacttac
      241 aaatgcaaaa ctttttaccg aacagcagtt tatttgcgta agccgaccac gtcgtttcgg
      301 caaatcaatg gcagcaaata tgctcaccgc ctattacagc cgtggctgtg attctcgtaa
      361 gatgttatct gagctaaaga tcgcaaaatc tgctgatttt gaaaagcatc tgaacaagta
      421 taatgttatt catataaata tggtcgattt tcttgaccgt tctaaaacta tggacgaaat
      481 gcttgactat ctcaggcgca gattgcttca cgagctgaaa aagggctttt cagacgttga
      541 ttgttttgac tgggataatc tgcaatctgt gttaggcgaa atatatactg acaaacgaat
      601 tgcttttatt ttcataatcg acgaatggga ctgcatattc cgtatacaca aaaacgattc
      661 tgacgctcag acaaaatatc ttgactttct gcgaaatctt ttgaaagacc aaagctatgt
      721 tgcgctcgca tatatgacag gcattttgcc cataaaaaag tacggcgaac attctgcatt
      781 aaatatgttt acggaggttt cgatgacaaa tccccgtgag tatgcagagt atacaggctt
      841 tactgaggac gaagtcaagg ggctttgtga aaaatataat atgccttttg atgaaactaa
      901 gcgttggtat gacggctata atttaaaagg cattgcaaca tacaatcctc gttcggtggt
      961 aatgtcaatg acaggtcacg attttgacag ctattggact tccacagaaa cctatgaggc
     1021 gctgaaagtc tacattgata tgaattttga cgggcttaaa gacaaagtga cacggcttgt
     1081 ggcaggcgag aaaataccga taaacccgac aaaatttcag aatgatatga ctactctgcg
     1141 cagtgcggac gatattttca cgctgcttgt ccaccttggt tatctgactt ttgattttta
     1201 cacaaagtgc gtgtggattc caaacagcga agtcgctcag gagtttataa actctatcga
     1261 agacggcggc tgggaagaag ttatgaaagc tatcaatgct tccgacaagc ttttgcaggc
     1321 tactataaat ggtgatgaac aggctgttgc tgaccttatc aataaagcgc acagcgagaa
     1381 tacatctatt ctgaaataca atgatgagaa ctctctttcc tgcgttattt ccattgctta
     1441 ttactctgca aagaggacat atacagtgga gcgtgaactg cctgcgggca agggttatgc
     1501 tgatcttgtt ttcaagccaa gaagaaataa cagcaaccct gcgatgatag tcgaactgaa
     1561 atacaacaaa tctgctgagt cagcgataca gcagataaag gacaaaaatt atcctggttg
     1621 cttgaaagat tattcgggcg aggtcttgct tgtggggatc agttatgatg ataataagag
     1681 acattgttgc aggatagaaa aaatagtttg ccaaaaataa tacaacattt gtatttgcat
     1741 ttttatgtta acaaaaactg gaatcgaggt atgttttatg agtattattt caatcatcac
     1801 cgaaaagggc ggcgtgggta aaacaactac cgcaattaac gccggaagtg cgttggtcaa
     1861 aaaaggcttt aaagttctgc ttatagatat ggataagcaa cggaatctca cctctactgc
     1921 aaatatcact gctgatgggc aatatagaga aattcatagg accatagttg atattttttg
     1981 tgaacttatt aaaggcagaa aacccgacta ttcagaattt gtacatcata atgacatcaa
     2041 tggttttgac tacatcgctt ctgatatgtt tgtggataat ttaaactcaa tgattgatga
     2101 ctatgccaaa acaaacggct ataatgtttt acaaagaatg tttaaagacc catttttcca
     2161 gtcctatgat tatattattt tcgacagcaa aaagtctaca gatagttttt caaaatgcca
     2221 gattgattct tcagactgtg cgataattcc tgttgaacca tcagcatatg cttttgatgc
     2281 tgtcgataga ataattgata agatatacca aaataatcac acaaagataa tgggcatatt
     2341 gataaacaaa acaggtcaaa cagcgctggc aaaggatttc ttgagcatat ataatgatac
     2401 gtataaggaa cttgtattca aaaccaaaat acctcaacgt gctgctcagg tcgagaaaac
     2461 accgtttgac ggcatcgctt gcgtcaacca atcgcataac actcttgcag aatattatat
     2521 ggctctcgca gatgaaatta tagaaagaac ttaaaggaac tgaataaaat ggtaagtaaa
     2581 caaaatatac ttctgcgata gcaaataggc gacaagtcaa tcacgctacc gatgttcgaa
     2641 ctcaaaacaa tttctattct aggcttaccc agagatataa caaatctgct gagtcagcga
     2701 tacagcagat aaaggacaag aattatcctg attgcttgaa agattattca ggcgaggtct
     2761 tgtttgtggg gatcagttat gatgataata agagtcattg ttgcaggata gaaaaaatag
     2821 tttgccaaaa ttaatatgac atttgaattt ttgaagccac gtatttcagt gttgataatc
     2881 gagacttgat aattcagttt gagattgaat gtactgaaat tgcaagagaa tgtgatttat
     2941 gaaaagaggt caagtgcaat gccaaacata tggtacaaaa acaaagaaac cgatactatt
     3001 tggtggaaag atgaccctac agttatcgga gcatggatat tcagctttga caaaaccact
     3061 gaatttaata tgttccatga ttatccttat aaattaacaa aagagcagaa acagattttt
     3121 gataaagaaa atcccgaatg ggcggatttc tttaaggata gaaaataaca gatacaaggt
     3181 attcattttg taattagata aatctctgat atccaacata ataaaaaagc ccacagaccc
     3241 gatcacattg atcgcatctg tgggcttttg aaattttacc acacatcata tatcttagcc
     3301 ttttcctcgt tgaaatcatc aggaagcgtt ccgatatccc agccggagtt ggtagtttct
     3361 atgtaaagat aatggcttcc gttgtaatca tagtacgtgc cgggggcatc gtctgttgcc
     3421 tttactccta ttgcaacgtg ttcaggaagc tcaataaaaa tgcagccata gccaagctct
     3481 ttgagcaaag ctcccaaaag tatggacgag tcctcacaat caccgcactg gtcataaagc
     3541 gtttccagag gatattttgg aaaatcagta tatcccgttg aggtcatgtc gtcaacatac
     3601 tctatgctct gcacgaaatt tgcagtttct cttatagtct cagattcgcc ataaccgcat
     3661 tggtcgcaaa ggctttttat ttcgtctgca agctgtttta cgagcttatg attattgtcg
     3721 tcacttacat agttatgata atctttcaca tatgagtatc tcaaaagact atgataatat
     3781 tcatacatat catcctgaat attcattgat atttcaaagg tatatcgttt gtcatagctc
     3841 gtccagttaa aagtcctttt cgaccatgtg gggtctgcag gctgaacata gtcaggcttt
     3901 cggtcgtcac gaggctcata tccgtcctca tcagtgacta tgggtgtttg ctgagtaact
     3961 gcggtagtgg tctgctgtgt tgttgtggtg tatgtgtcgt atgtattcaa tcccggtctt
     4021 ttctctggat acagaaattc atcaattgta ttgctaaatg caaaatatac gcccactgcc
     4081 acacaaatag agataagtaa tttcttcata tattcgtcct gtttttagtt ggtttacgac
     4141 ttttgtcata tgtacattat actacaagac ggtataaaat acaatactat tagatatact
     4201 ttacgaattt gttcttattt ttgctatggt ttgatatttt gctaaatagt tgaaatttgt
     4261 gaggcggtgg atttcattct cacctttcgt gaatttctgt tatattcata gcatctgacc
     4321 taacggaaca ttttttgtga caatgaagat ctatgccatt gtcgatagag tcctttatgg
     4381 ctagtttgtg tatgaaaaaa gcacccgtta aggtgcttag ttccgatgtt tgggtataaa
     4441 aataccgccc gaccttagtc aagcggtaaa attatttatg ccgtttctct ttatgcgata
     4501 aagagttccg aacctgtttc gatatcgcaa gctttcaaca gctcagttgt actttttgca
     4561 atatctgacg ggtcattgta tcttgtcatt gctactgaaa cgacttcata tgtcctgcca
     4621 ctcttatcgt acaacttcgt accatttttg agcatttcac acttgccgtc caaagtaaca
     4681 gacaacatat cgctgatttt aaatacatca agaactttaa acatatcagt cgcctccttt
     4741 ttcattatat gcctttaatt cactttcata ctgttgcaaa gcaatttttg tttgttcgac
     4801 ctcagcttct gtcaacttat aagccttgtt gtttcgtaaa agctttttct gtgccttgat
     4861 ctcgcaatta agtcttgaaa cataacttcc gtcattttct ccgttgcgat attgtgttgc
     4921 gtggataagt tcttcaaaaa cactggctct gcctggattt tgtttaagca atatggtctt
     4981 gtcattgtaa gtaatagcct cagcattctt acttttcagg taagcgtctg tttcttcggc
     5041 ataggctcaa tatagcctgc tctggttttt ctatacattg cttcacttcc tgcattcatt
     5101 ataccatatt tttcgttttt gtcaacacca ccgccataat acagtataca gatttctgga
     5161 atataacaaa aagagaatta attaaaaaga cacaaaaaag tgatatttga agatagccaa
     5221 gaattcttgc aaaatctttt tgacatagca aaaaaagaaa gcatatatat ttcccagaaa
     5281 aagatacaag cagcaccacg acagatcacc ataacac
//
LOCUS       DAWEMR010000179         5316 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_5931_length_5316_cov_8.755180, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000179 DAWEMR010000000
VERSION     DAWEMR010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5931_length_5316_cov_8.755180"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1549 a         1213 c         1118 g         1436 t
ORIGIN      
        1 gacatactcc tgatgttcca atggtcattc ctgaggtaaa ccctgagcat atcgagataa
       61 tcgcttcaca gagaaaaaga cttggcacaa agagaggatt tatcgcagtt aagtctaact
      121 gctcactcca gtcttacgtt cctgctcttg ctcctcttat ggataagtat aaggtaacaa
      181 aggctcttgc ctgcacatat caggcaattt caggcgcagg aaagacattt gaaagtttcc
      241 ctgagatact tgacaacgtt atcccttata tcggcggcga ggaagaaaag tctgagaaag
      301 agcctcttaa gatctggggt catatcgagg gcgacgttat cgttgacgca acagctccag
      361 ctatcacaac acagtgcctg agagttcctg tttccgacgg tcacacagca gcagttttcg
      421 tatcatttga gaataaacct tctaaggaag agatccttca ggcttggaaa gatttcagcg
      481 gtgttccaca ggagcttcag cttccaagcg ctccaaagca gttccttaac tacttcacag
      541 aggacgatcg tccacaggct aagctcgaca gaaaccttga gggcggcatg gcagtttcta
      601 taggcagact gagagaagat tcacagtatg actataagtt cgtttgcctt tcacacaata
      661 cactgagagg tgcggcaggc ggagcagttc tgctggctga gcttcttgca gcaaagggct
      721 attttgacta atataacaca tatatacgca cgaaaggagt gctgtttatg aaaaagacta
      781 ttttcacagg tgcaggcgtt gctatcgtta cacctatgaa cgctgacggc tctatcaact
      841 ttgacaagct gggtgagctt atcgatttca atatcgataa cggcacagac gctatcataa
      901 tctgcggaac aacaggcgaa agtgctacca tgacagacga ggagcatatc gagtgtatcc
      961 gctatgctgt tgaaaagaca aatcacagag ttcctgttat cgcaggcaca ggctcaaacc
     1021 atacagagta tgccgtaaac ctttctaaga aggctgagga gcttggtgct gacgctctgc
     1081 tttgtgttac accttattat aataagactt cacaggcagg acttatcgca cacttctccg
     1141 ctatcgcaaa ggctgttaca ctgcctatca ttctttacaa cgtaccgtca agaacaggtg
     1201 tgaacattct tcctgaaact tgccgtgagc ttgcaaagat agacaatatc gttgctatca
     1261 aggcagcaag cggaaacatc agccagataa cacaggtggc agctctctgc ggcgatgacc
     1321 ttgatatcta ttcaggcaac gacgatcaga tagttcctat catggctctg ggcggaaagg
     1381 gtgttatctc agttctttcc aactgtatgc catttgaaac acatgagatc acacagctct
     1441 gccttgatgg aaagtacgag gaagcaagag aaaaggtatt caagaccctt ggttttgcaa
     1501 aggcactttt cagcgacgta aaccctatcc ctgtcaagga agctcttaac cttatgggct
     1561 ttgaggtcgg cgagtgtcgt ctgccgcttg taagaatgag cgatgataag atagaaaagc
     1621 tgaaagcgtc aatgaacgct ctcggactta tcaagtaaaa tatacaaagg gcggagaaat
     1681 tcgcccttta tgtttaccgt tataaaaatg tactaatgta agggttggcg tccccgacaa
     1741 cccaaacgct gtctttgaca ttatcactta cgtttatcta caaatcaaaa agaaaaggaa
     1801 tgatataaat gactaagatc gcaatatgtg gcgcctgcgg aaaaatgggc aggttcatat
     1861 atgacgttat gtcagagcgt gacgactgca cagtaagcgc aggtatcgac aagttcggcg
     1921 aggcttatgc agacttccct gttgtaaaag agcctgccga cctgcctgaa aagcctgatg
     1981 taataatcga cttctcacac ccttccgcac tggacggact tctcagctat tgcctttcaa
     2041 acggcactgc ccttgtaata gctaccacag gctatacaga agaggataca gcgaagatac
     2101 acaaggcttc cgagcagata cctgttttct tcacattcaa catgaccctt ggtatcaatc
     2161 ttcttgcaaa tcttgcaaaa actgccgcaa aagttcttgg cggtcagtat gatatcgaga
     2221 taatcgagaa gcaccataac cagaagatag acgctccgtc aggcactgct atcatgcttg
     2281 cagacgcaat aaacgaggag cttgacaaca agtatcacta tgtttacgat cgtcattctg
     2341 taagagcaaa gagagaaaag accgagatcg gtatgcactc tgtccgtggt ggcactattg
     2401 ttggcgagca tgagataatc tttgcaggca gagatgaagt catctccctc aagcacgaag
     2461 ctcattcaaa gcaggttttc gctgtgggtg cagtgaatgc aggcgttttc ctttgtggaa
     2521 agccggcagg actttacgca atgagcgatc ttcttgcttc actttaatta aacatcagat
     2581 acagtgggcg tcattgacgt cctgctcttt cggagatttt ttacactctc aggctgctgt
     2641 tttatacggc agcctttttt catacaaaaa aatagccgtg cgtgattttt tccgcacagc
     2701 catttcatta catattctta tcaagctcca aagtagtttt ctcacgcttt gtgagctttt
     2761 tccattgtgc tttgtactct gcatagacca ttttccactc ggcttcatca ggaacatatc
     2821 caccgaagca ggtcttttca aaaagatcta taagaccgcc aagctcaact ccacgcctct
     2881 taacaagata ttctcttatc atttcaggcg tgtatcccaa tagcttatca ttagaagata
     2941 tggtcaaaag ccttaccacc cgcctgtaaa gccttatgac tttgtcgccg ttatctctga
     3001 atttaagtga cagtctgaaa aatacctcga taattctgtt aaggaatacg aaaaccacaa
     3061 gcacaaacgc tacaccgata aacagtgcga tcctgctgaa atagcttaca aaagttgaaa
     3121 catcaaagtt cgtaccattt gcactgtagt cacgctgata gcccggaact gtcgggtcaa
     3181 aagtcatcca gccaatgcct gtgataaaga cctctacaaa agcgtgagca tggctatctc
     3241 ttaccacaaa ctctccacgt tcattctttt catatgctgc aaagccctca acatatcttg
     3301 ttggaagtcc cagcattctc gccataagcg tcatgcttgt ggcatagttc gtgcagttac
     3361 cgcgcttgct ggtgaaaaga aaatatgcga tagactcatc gtcaggctca tactcgttat
     3421 cgtaaacata gccgttctgc tcaaaataat ccacaagcgc ctgagctttc tcataatcat
     3481 ttttgcaatt ttccgttatg tttttcgcaa gagcctccat ttttccgcct ttatcgtcat
     3541 atgcagaaac atctgtgtac aacgcatata tcttataaat attcagcgct tgatttgatg
     3601 tgacatctcc gtttgtcaaa ccataagaaa gcagaagttt gaactgattt tcggtcatat
     3661 caagatttgc cgcagcctgt ctttcgctct ccgtttcagg gatatactca aagctaatgc
     3721 caagcttatt tcctgcggtg tcatatggtc tgtagacctc actgtgagtt gttttgatat
     3781 aaacgttgtt ctcccctggg gttatcatca gtggagcagg gatatagcta ggggtaaagc
     3841 tgtcgctgaa ctgattcatg actgcctttt cactgaacag atcatcagga aactgtgacc
     3901 tgtccatgcc aagatccttg gtgtaatcct tatctgcgag tgactttgct atgctgtaaa
     3961 catagctcgg tgagttcacc tcgttgtcac cgacttctcg tttttgaaaa ggtcaaagga
     4021 ttgtctgcgg aggtaaatga cctgttcatc agaggttgtg gtgaagttga aaagaatctc
     4081 gcccgttgcg tctgcgccaa atctttggga agactcatca ctaagattat catatgtgcc
     4141 actgcttttg atattcacat caaagaagtt gctgtttttt tcaagataag atgtctttga
     4201 ggaagtcggc ataagcattg caagcgcacc cacaagggta acgaaaaccg ccgatgccgc
     4261 cacataagag ccgtttatga caaggtcatg cttgctctcg tctgaaacaa gtctgctgtg
     4321 caccataagt gcaataaaca cggtgagcat tatggttatg tcaagagtat ccattgagtc
     4381 ggcacgcttt ccgtaaataa aaaacggaaa aagcacaacg agcatcgttc caaagcttct
     4441 atagcgcaca atggtgaaat agtaaagcac cgaaatgata aagaagccta gtcccacaaa
     4501 tagaaagtag aaatattcat ctatctgacc tatctcctgc ctgtttacat aaaaccaatt
     4561 gataaatgat tttccagaat gttcaaatcc agcacgaaga agctgaaagc ccaaaaacag
     4621 cactgccacc ataagggcgg tatagacaag acctcgcagg acttttttcg gcttcagcca
     4681 ctcaaaaaga gcgaacagca gacattcata gaccacatat ccaaaagtat aaagtctgac
     4741 attgtctaca agataggtct taaagattat gttcatgacc actgctccaa ggagcagcgg
     4801 caaagcatat tttgaaagaa tattcacaag ggtgtttttt atctgagcag ttttagtatt
     4861 caaaagccac accccctatg tcattttacg aaactcgaaa gtttcgtcta caagccatat
     4921 attttctgtc tgcttatcaa gaccgctaag acgtgtcact acaaaagaac cgttaaattc
     4981 ttccataaga gaagaaaaat ccccctgaga agtcgccatg caggctgtga agcagatagg
     5041 agctgttttg cctatctcat gagaaaaggc aaaacgcttt tcctgcggat atggtgctat
     5101 tgccgcaagg tcgttttgaa gctctattat atcacctgcc tgctttattt tcatgctttt
     5161 ccagccgtca agataataat tatactcact ctcaaagccc tccatgataa gcattgaaag
     5221 cattgcaagg ctcgcttcga ctatattatc aatattcata gcaaacatag ggtcatgatt
     5281 ttcgcttggt atatcaagga aaaacgcctg acttga
//
LOCUS       DAWEMR010000180         5142 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6150_length_5142_cov_7.009829, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000180 DAWEMR010000000
VERSION     DAWEMR010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6150_length_5142_cov_7.009829"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1741 a          793 c         1030 g         1578 t
ORIGIN      
        1 gacaatgctt atcttgccga gttacataaa ccaatgcgaa aaatgtatgt taaaatggaa
       61 attttatcac actatgaagg tgctattggt gaaataacaa gcgatttatc ttctacagat
      121 ggttcaataa cgattaataa agagcaaggc tgtcgtaggt cttgctcttt atctattatt
      181 gatagaagtg gcaaatacat acctcaaaaa aatagtccat tttggtacaa tcgaaaattc
      241 aaaatcttta ttggattgca agttgatgag aatatttatt ggtttccgca aggtgttttt
      301 gttacaaagt cagcaaactc taatggtaga cgattaaatg ttgagggtgt tgataaatat
      361 ggttttcttg acggaacatt aaattcaaga atgtgtcttg tcgagtatca ggctagtgta
      421 actaattcta aaaaaggaac gaatattgcg actttaatta aggacacgct tatgcttgat
      481 ttgggtaata atatacctct tgaccctgtt gagcctatta ttgaccctat tttctataat
      541 gtaactctgt atgacgatat tgtagttgat gagggcggtt atcttggtga gattttcgac
      601 aagattgccg aaatgtatgg tgctaacatt tattacgatg ttaatggtag attgagaatg
      661 gaaagagttt tcaactataa tttaccttct tggtatcgtc atttgtcacc acaatttgaa
      721 ttgagtgaaa ccgaaattac agaaacgggt attaattata cttataatta tgacggtgta
      781 aacattatca cagttacaac agacaataca aatggtgaaa tttattcgta cacagctaaa
      841 aatgaaaacc cacaatcacc tgtaaatata aatgctgttg gttataaggg tttggacggt
      901 ggtacttatt atatacccct aggagataca agtgaagaaa gcggagagga aaagtgtagg
      961 caacaagccg aatatatgtt attgcaacat acttgcatga gtacaggtat tagttataat
     1021 ctgccgatta ctccacattt gaatgttgat aataccgtta gggttagcaa tgattattat
     1081 aattttgaca aacagttatt tatcgtaaac tctattacaa tgcctttatc ggcttctgaa
     1141 atgagtattg aagccactaa tctgcaatgg ctaccatttg atacagattg tatttcgatt
     1201 tactgtgaaa attcgagtga tacagtggca atatcttaca atacgaatgg tggcaaggac
     1261 aaagacggta atactatcac ttataagagt atcagccaag cccctaataa acaagtcgtt
     1321 ttacaaggtg gggatatgta taacgagaat aaattatttg cgtggacgga tagtcaaggc
     1381 aataaataca attatggtga cgtgtacact gtaccaaata acaatgcaac actgacagct
     1441 caatggataa caggaaatga agttacagtt accaatacat tatcggcaga tagtacggta
     1501 gaatttcaat ctatgtcacc gtcacgttgt ttaatacgct atgatgacaa cgaagtagtc
     1561 agacgtaata caaacacaat ttcaatattt aaaaagaatt attctttggg tacacatgat
     1621 acaactattg tgtctgagag tgatgattta actaactttg acaatgcttt tgataaagaa
     1681 acaactacaa agatagattg ttccaaagta aaagctacct acctcacttc acctatggga
     1741 aacggatttg agaatatgac agattttgtt ttccctgcta atctcgcaaa cattacgacc
     1801 agtaagggtg tgttgtcggg ttgtaaaaag cttgcaaata ttacatttcc tacagtatac
     1861 tgtgatattt cgcaccccga atcgtttctt gctaatagca cattcgttaa tggtttggaa
     1921 ctacctcaca ccttgaattt cgtaccaatg gtttcagttg ataagcaaac aggtatcgaa
     1981 gaaacaaaac aaaacgagat actaaaagga agtcatgttg ttagaagcct aaacatcaaa
     2041 gcatcaacta cgaataaatg tgtagtgtat gtaaataaag aaacaacaag tttagttatt
     2101 tatcctgcga cagtgcaggg aagattctat cttatggaca aaggtattga tggagattta
     2161 tctggacttc aaagtataca aattgggcga tctaccaata ttaacgatac agacggtttc
     2221 gcaagtaata catcggcaaa tataaatctg agtttagact tgcaatcggg taattgtact
     2281 accaaaatac ctaaaaacgc ttttaatggc tatagtggta acacgattaa tgttgtgatt
     2341 tatggtaatg tgaccgacag caatggtatt acgcttgaga gcggatcgtt ctgcaatatg
     2401 cccaatatga caaaattgcc aatgacaaat agtacaagct taaaagttat acctgagaac
     2461 tgtatgaata atttagtctc attaacttca gcgactacag gctatgtggt tgacgttgag
     2521 ggttgtaacg atatgcccaa tctgacaaca ttaagaattg aaagttcttg tgagaaggta
     2581 aatgggttta ataactgccc taaattgaaa agtttgtcat tcatgagtga cggaaaagta
     2641 aaagagattg gtgggttaaa caataacgct attacaacat tttatattcc aaatacggct
     2701 ttatctgtat cgggcgtgaa taattgctct gcattaacaa cggttgctat tggagcttct
     2761 ttgactagct ttacagggtt taataattgt cctaaattaa acaagtttac tgtggatagt
     2821 tctaatacta ctttcagagt cgttgagaat aacctctacc aagggaataa actctgccgt
     2881 gttccaatga gtaggtcaga tattgtagta gcaaatggta caacggaaat catgagcaat
     2941 gccattcagg ttgcttttgc aagcagcatt tccattccaa atgattgcat tttagctaac
     3001 gactcaatca aatgtcaaag cgtaggtcaa attattttcc atacttcttt caacatagaa
     3061 actgggaaat ataataattt aactatgacc gattttagta cccttgataa cgtacaagtc
     3121 ggaactattt tcacatatgg aaatggtata acagatacta caaacgcaaa ttgtttgcct
     3181 attgtaaaat actgtgtgga acataacatc aattatgtcg atatgaacga aacaaatact
     3241 aacgctcgtg gagctattgg aataagcggt aatgcagaat tggacggtga taattaatga
     3301 taaatactta tacttgcata ccaaatcaaa cttcatcaga aaccgtgttt gcagacctta
     3361 aaacattttt tgaagataag tggacttgga gtaaaattga aacaaattat cctgatagtg
     3421 agtccaccga ttataacact ttgacatttt ggattgataa tacaacgtac tttagaataa
     3481 tgtttgaccc tgcaaagtcg cgctactggg ttggatgtgg tgaatatgac tctactaaaa
     3541 cgtcaccata tgctgattat gtcagcttcg cctataacaa gtttgatagt gtcatgttgt
     3601 atactacaag tcggggaatg ttgattttgt ttaaaagtgg agataatgac tatgtattag
     3661 gtggggctat tgcaaaaatg agaaaactat ctgacaatac agaaattaca ggtttcttta
     3721 cccctacttc aaattcagga catcaaggaa gtaaaatggc aagcttatat aatatgttta
     3781 gtcaaagttt gcacaatggc ggtacgaacc ttgtaccaca agttgatttt aatataccat
     3841 tgaatagcac agttgagggg caatacgctg ctaaaactga cggaatattc tatgtttata
     3901 tgggacaaga cagtgtgttt cctgctgacg gaactgttgt aaaatttaca atgaatggtg
     3961 ttaaatatgt aggtaactgt aaaatggttt tagctgatta ttcgtaaagg cggtgtacag
     4021 aatgtctaaa atgaataagc tgattaagga aagtcaagat aataaaaaaa cacttggtta
     4081 cacctatgga acagttaaaa gctatgactc tacaaactgc acggctgttg tttcgctatt
     4141 agagtataat ggtgctgaaa aatctttttt gaataaatca ggtgagattt taagcatggg
     4201 agacagtgtg tggatatatt tccgtggtgg cggtataaac gctggctaca ttgctatcag
     4261 gaatggcaag cccgtacctc taggaagtca aaattctagt gtagggcgat ttgttgaata
     4321 cattgatagt ggtggtagta gacacatctc agaaaagttt aattattatg gcgattctta
     4381 tataatccct ggtggaacag ggcagattat ttatcttgaa aatattgctc atggtgatta
     4441 taaccatgtt gaaggtcaag caaatcactg ctacgactat agttatgaca gcaataatta
     4501 tattgatttt tcagacatga aaagcaattt aatgctgaag atgttggtga actatgctcg
     4561 tggaaatagc agtttaaatt ccttaacagg ctttaataat actagcgttg gtggattttc
     4621 taatcacgtc agcggtatgt ggaatacgtc taaatatagt gtggcagttg attgcagtgg
     4681 tgcaacaaat actgtttcca attctcgtgg tacatatgtt aatggtatag acaatgagct
     4741 tgaaggcgtg tccgatagta ttgtagttgg tacatataat attgttaagg gtgacaaaac
     4801 taaagaccaa atggcaaaat ataacgccgt gtttggatat cacaatgatg tttttaatta
     4861 tgatggatgt cttgtcgcag gtttatggaa tcacgccacg gcagataacc aaaccgttat
     4921 aggtattaat gcaaaatcaa cttataaaag ctcggaaatt gcaagtatac tatttaatat
     4981 agggaacggt cataatatag aagatggttc tctaactcaa aattctgcaa tgcaagtgga
     5041 cttttcaggc aatgtttatg ctggcggtgc atacaaaact attggcgctg actatgccga
     5101 atattttgaa tggcttgacg gtaacactaa aaatcaagac ag
//
LOCUS       DAWEMR010000181         5067 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6237_length_5067_cov_11.103751, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000181 DAWEMR010000000
VERSION     DAWEMR010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6237_length_5067_cov_11.103751"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1640 a          876 c         1231 g         1320 t
ORIGIN      
        1 tttcagggca atgctcaaat ggtgcaaaaa tgattataga tttgacctgt tcagagatgt
       61 aatgctttcg cctgttttcc aatatgagga gaaagtagag gacggcaaga ccaaaagcaa
      121 gggcaggata ttccttgaag cacagaactc tggaaacggc tggggcattg aatggtatag
      181 cacaagggat agtcaggtgt tcactgaatt ttatgatgtc tttaaaaaag attatgtcag
      241 cgcaaaagag ttttacggtg gcgtgctgaa attggtgaat gattatgtga aaggtacaaa
      301 cgctgagcag agaagttcca tatcaagcgt aaaggacgct ttgataggca gatacagatt
      361 ttatgcagag tatgacaaga gtcttagctt gcaggaagca atgactgaga tcaccgacat
      421 tgcagagaac gcaaggtatt ctttgcctgc atcaaaaggc gctgttacct gatgtcttat
      481 ggggcagtat tctgcgcttt tcatgaaaaa catatatgtt cttggtttga cgacaggaag
      541 atttcctgtc atgcaggacg agtcacctgt tctgaacgat aaggagagag aacagctttt
      601 cggcaactgt gaccattgtt ctgacgctat cgagcgcaga aagctcactg accttgtgag
      661 aactgttctc atggctgaga gagagaacct tgttgtcagc tgtagtgttt atgataccgt
      721 agaaataaga gcgcaaagcc cgtcggcagt gtatactgtt attgcggctg aaaagggcat
      781 tgatgtaaac aaaatagaag tgtatgatta tctttccgac agacgcaagg ttagcgtgcg
      841 ttctgagata tcattaaaca gtgcttttct taacaaggca gaaaaaatcg ggcttcatga
      901 tgacggtgcg aagcaaagcg aaagccttac ggagtatctt gataagcaaa gaaaaagccg
      961 tatagatata ttgcgtgaaa tgtgcgaaaa taaacatttt atcatttctg ccacaagcct
     1021 ttcgactatg cttcagtgtc cgctgaggtt tttctatgag cgtatcgctc acgttgaaaa
     1081 gccgcaggag gtggatatag accgcagtgc atggctcaaa ggcaatgtca agggaatgta
     1141 tatacatgag ataatggaga attatgccaa gactgtgctt aaaggcaaag gtgcggctga
     1201 ggtgtctgaa attgttgaca cagcgatgct tgatgagata ttcgagagcg tttctaaaaa
     1261 ctatctccgt aactatcccc ctgtggatat ggctgttgcg cagagtgaga gagaggaata
     1321 ttacaactgt gtcaaagctt atcttgacga actgcatgac gatatacact caggcaaacg
     1381 tgaggtcgtt gaggtcgagt atccgttcaa cggtgatgtg gagataggca aatatacagt
     1441 caccataagg ggtaggatag accgccttga caggataata gacggcacaa aggtgtcata
     1501 tcagatagtt gactacaaga cggaggatat caaaaaattc agggagaagc ttccagaaga
     1561 cccacaggat tatatttatg cccttgcact tgaaaaaggt cttacgcctg tgggcgaagt
     1621 gagcgaaagt gatatcgttt ccgcagatta tgttttcatt cttgaaaaag ggaattgcca
     1681 tgtagttaac aataaggata attctggaat tgaaaacatg ataacaacag catttgataa
     1741 aatattagaa gagggcttta aaaagtgtat tgtgaataag aatgataaaa ttactccatg
     1801 cacgttttgt cctgcttatg aagaagtttg ctcagggggt gcaacagaat gagtgaagaa
     1861 cagataagaa gctatataat agacaaagac agttatgata agaatatcct tgttgaagca
     1921 ggtgcaggtg caggtaagac aaggcttata atacagcgtg ttataggtca gataggcgca
     1981 ggtatacctg ttgaaaagat agtccttata accttcacca acgctgctgc aaacgagctt
     2041 tatgagagga tacaggcagg gcttacggag aatattgcaa aatgcagcgg tgaggaaaag
     2101 aagctgtatg agaacgctgt tatgaattta cagcgcatga agataagcac tatacacagc
     2161 ttttgtttga gtatgctgtc agaacagcct tttgaagctg acctgcattt gggattaaaa
     2221 cttgccgagg acggtgaaac aaagcaggca caggctgact ttttcgagag atattaccgc
     2281 acaaatggtg cgaacataga agatttcggc tttctgaaca gcaatcaata taagctcaga
     2341 gacagccgtg tgaaaaactt tcttatggaa acatttcttg agtgtgctga atacagagat
     2401 gttgagtttg ttagggataa aagctatgac agtgtgacat ttgacaccat aaataagctt
     2461 gcctataaag aaatgtgtaa ctatagggat agtcttgtgc gtgcattcta taatggtgct
     2521 ttgggtggaa aatacgaaga tatagcaggt tttcttgttg atgatgtgac gaagaaatta
     2581 cctgatgata tgaagaccta tgagccaaag tataattatg taaaggtttg cgctaatctt
     2641 aaagcttgca aaaagttcct tgagcctttt aataagacta aaattaagaa agatcatcca
     2701 aagagcaatg agaacatggc tattttcagc ccaagtgggt ttgataatgt aaagaaagaa
     2761 aatgcactgt atgccctcaa caagacggca acgtatatag agcaggctgt aaaggcttat
     2821 caggagagtg aagacgggga gagcgtttca aatgacgggc ttttgtatca cactcacgag
     2881 cttctcaaaa acagcagtga ggcaagggcg tttttcaaaa acaagtattc ctgcatttat
     2941 atagatgagt ttcaggatac tgatgttatg cagactgagc ttctgctgta tctttgtgca
     3001 gacgacagca agcttatagc aggcggtgat atatggcaat gcccactccg tgatggtgcg
     3061 ctgtttgcgg tgggcgaccc taaacagtct atctacggct tccgtgacgc cgatatcagg
     3121 ctttataaca aaatgaaagc caaattccag aacaataatg agaaaaactg cgagtttttc
     3181 tcacttgatt acaacttccg ctctgatgag cagcttatag cttgggtaaa tgaaaagttc
     3241 cggcctgaaa taaacggcaa atatggtatg acatacagcg atatgatatc aaaggctgat
     3301 aagcctgcgg ctgtttccga tgatgttatc agaggggcat attttgtcaa agatccaggt
     3361 gctgaaagcg tggctaagat aatatcagac ctagtgaagg tcggaaagat attcgacaag
     3421 ggtatggaaa gacctataaa atacagtgat tttcttctgc ttctttggaa cacaactcaa
     3481 atggagtttt attcaatggt cttgacagat tgtggcatac catatacaat gtggggcaaa
     3541 cgtcctcaaa aaaacagtct ggtgctgaaa agatttattg ctcttgcaga gttcgttttt
     3601 gaaagctaca gtcctaccgc aaaggaaaag gcttattcta tcctttggag atgtacgcct
     3661 gacagcatcg attttgaaga cgctgtaatt gacgtagacg attatgaaca aaactgtgaa
     3721 agcaatgacg ccacgcttgt tgcggtaaag gcgtctgccg atactgtcaa tgagcctata
     3781 gcaaagcttt ataagattct tgattttcct gagcttcttt ttgataatac cagcaacaga
     3841 gaatatgagc tttcaaatct tatctatctt attgaaaagt gggaaaagca ggattttgca
     3901 ggaagcgatg agcttatgga catgatgaaa aacgagcttt ctgtgctgaa agaaaaagag
     3961 cttatccttg acagcagtga gcagaactgc gtaaggctca tgaatcttca caaggcaaag
     4021 ggtcttgagg gaaatatcgt tattatggcg gaggctaaaa atcataaatt cagcggtgga
     4081 aagtacaccg accttgtggc taaaagacag gtctatgtac ctttgaaaac aagcgatttt
     4141 tcatatatgg actatctgac attctaccct aatgagagga agcaggcaga ggataaatat
     4201 tttggcgaac agctcaggct tgaatatgtt gcggccacaa gagcaaagca ggcttttata
     4261 tttgtgtata cttttgaacg gaaaaaaact gtgatcgaca aacttacaat gagcaaaata
     4321 ggcagagaac tgccgcagat aaacaatgcc acagcgcaaa gtgctgtgca ggcggcgcag
     4381 ataaacgtca aggatatatt cgacgaggtg aacaacagcc gaaatgcttt tgatgaaagc
     4441 gttaaaaagc tcagtgagaa acatgttagc aaaatcactc ctagccacct tgaggacggc
     4501 aaaaaagtca ctataccaag tggagtggag aaactgtctt acaaacgtcc aaagggcata
     4561 gatatcggtc atatggtaca tcgtgccttt gagcttgcgg tgaatatgag tggaaagctg
     4621 tttccgtata acgaaacgcc acagcagaag gagcttattc agaagattgt cagcagagcg
     4681 tatctcgact ttgaggacga gtgcagttcg caggattctg agagctttta taaggactat
     4741 atcaactatc agcttcacag ttttttgaaa gacaaggata tcgaaaaatt gctcaaaact
     4801 gcgcaggcag tgtatacaga gcttccattc tacagtatgg acggcaatga atacagcaac
     4861 ggagttatgg atcttgtgct taaaatgtca gacaaaagct ttatcataat cgactataag
     4921 accaacatac aggaagaagc cgacagaaag ctttttgagg agagaatttt aaagacatat
     4981 cgttcacagc ttgatttcta cgaaaagata ctcagaaaaa tgctctcgct cagtgaggaa
     5041 actaaggtgg agtgccgtat ttacttt
//
LOCUS       DAWEMR010000182         5018 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6301_length_5018_cov_10.128954, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000182 DAWEMR010000000
VERSION     DAWEMR010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6301_length_5018_cov_10.128954"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1564 a          994 c         1088 g         1372 t
ORIGIN      
        1 tcacgctcca cgtgagcgtg tgagttgaaa ttgttatgat acggaaaaaa tatccaatgc
       61 aaccatgtca cgctccacgt gagcgtgtga gttgaaattt ccagtttgac ctctgctgtc
      121 agaatattcc agtcacgctc cacgtgagcg tgtgagttga aatgttagtt gcgttttgtc
      181 aatgtttgat acaccgaagt cacgctccac gtgagcgtgt gagttgaaat gttaagtcgt
      241 ctgctgtctt tgccaaactc atgccagtca cgctccacgt gagcgtgtga gttgaaatcg
      301 cccgtaatta tcaggtacat ttaaaatcgg ttggtcacgc tccacgtgag cgtgtgagtt
      361 gaaatagcct gtgtagctga atttttcaaa gctacattgt cacgctccac gtgagcgtgt
      421 gagttgaaat tcgtttatca ctgtaatcat tataatacat tctggtcacg ctccacgtga
      481 gcgtgtgagt tgaaatctcg gacgggtcaa gaatgacgtg tccgccctct ggtcacgctc
      541 cacgtgagcg tgtgagttga aattatcaac tgcacagcat ggaacaatac agccgtggtc
      601 acgctccacg tgagcgtgtg agttgaaatt catcataacc acaggtaaag agggggaatc
      661 ttgagtcacg ctccacgtga gcgtgtgagt tgaaatcaca cgcccctatg ggcgttctga
      721 aagtctattt gtcacgctcc acgtgagcgt gtgagttgaa atatgataat gtaaaaaaga
      781 gaggtatcat gcaatgatta aattattttt gaaagaatac ttagatgaaa tgtcaactgt
      841 atgtagggat aatcagaata atgtaagtat cgctgtaaat cccgattctg aaaggcaggg
      901 atatccatat ttcaaatttt ataattccgt ttattatgga gatgctgcta aggtggttag
      961 aattttattt aattcagcag attatgttga taataaaaat gcagaagacc aaaagctttg
     1021 gaaactttcg cataaagaaa aaaagttgct taaagaactt ttgagtagtc cttctgcaga
     1081 atattcagat atgactattt gggaagcttg taaatttgag tggaactttg aatatttaga
     1141 gcaatctatt aacttagata aatatgtaaa tggtgaatat gataaggata aaacattcac
     1201 agaaaatcct ggctatgtac catacagttt ggaaatgcct gattatttag aattaaactt
     1261 ttgctaaata tgtaacttta acgtacgctc cacgtgagcg tatgagttga aatgattgcg
     1321 gagctataac gcaggaagag tacgatagtc acgctccacg tgagcgtgtg agtcaatttt
     1381 cgtttcatcg caggacttag ggtgtacgtt atccgctcag accacagcaa gtgttgtgcg
     1441 tggtgttcaa agcttgcagt aaaagacgtt tatcctgagt ttaactggag aaaagggggc
     1501 tatgttcatg gcagaagaaa atgatattct cacaaagtta aaaaaaggcg aaaaagttga
     1561 gtgtcctgtt tgccataacg gctactatgt tcctgtgaat acaacagcaa ataaggcaca
     1621 ttactttgtc tgcactaaca aagagtgcaa cggacattat cggtgggagc cgataatcga
     1681 tattgaataa tacaaataac cgcttgacta aggtcgggcg gtatttttat acccaaatac
     1741 tatacatttt ttatgtgttt tccgtcaaat ccgccaattc acaccccaaa tttgtgcaaa
     1801 caaatgattt ataatttaat ttggcaacat ttctgcaatt gttgcatttt ttatgcaaca
     1861 aaagtggtgt ttttcgggat aagtataggg gtattatccc aaattgaata tcctgcaagg
     1921 tagcagtgta tgaggaaaca ttttgtcaac gtgagttagg tgaacacgat gagaggttct
     1981 gatcgcacag aaaagcactt cctgcactcc gtctgaagct tgaaaattga atatttatac
     2041 cgacacacct tagctatacc ccatgagcat actgctaaat ttaattgtta taaccgcctt
     2101 tgttgacatt attcatataa aatggtatac ttaaaataaa atttatacat aatgaaaaga
     2161 gtaaatgtga tgaataaaca taccactatc gataagtcaa caggcgagaa aaaatgtagc
     2221 tgctttaaac gctgcggcgg ctgtcagttg gataaggtct acgcccagca aatagaatgg
     2281 aagcaggcaa aagccgatag aatgttgagc cgcttttgca gacctcagca gataataact
     2341 atggacgacc cctataatta tcgcaataaa gtccaaactg tctataaggt caatagctca
     2401 aagcgtatcg tctcaggggt atatcagtcc tctacccata gcatggtaat aactgacgat
     2461 tgctttttgg aggatataag agcacagcaa atagtcgcta cccttaaaga gcttatggct
     2521 gacttccgta tccttccata taacgaggaa agtgatcagg gtcttcttaa gcataccctt
     2581 atccgtacct cccaaacaac aggcgaggta atgctcgtgc tagttacagc tacccccata
     2641 tttcctgcaa agaaaaattt cgttaaagct attactgaaa aacacccata tataaccact
     2701 atcgtccaaa acgtaaataa aggcagagtt cctcttactt tgggtgatcg tgatattata
     2761 atgtatggca aaggctatat caccgacgag atgtgcggct gtaagttcag aatttcacct
     2821 cattctttct atcaggtaaa ctcagagcag accgaaaagc tctattctaa agcagtttcc
     2881 tgcgcagatc ttaaaaaagg cgataaagtt atcgacgcat attgcggaac aggtactatc
     2941 ggtcttatcg ctgcaaaaag cggtgcagac gtggcaggcg ttgagcttaa tgccgaagcc
     3001 gtaaaggacg cagtttcaaa cgccaagctc aataagcttg acaatatcag gttttataat
     3061 gaggacgcag gtgagttcat gacaaagctt gcgcaggcag gtgagagctg tgatacagtt
     3121 ttccttgacc ctccaagagc aggtacaaca gaagaatttg ttgaagcctt gggcaaactc
     3181 agacctgaaa agatagtcta cgtttcctgt aaaatagaaa ctctcgagcg tgatttgaag
     3241 cttcttgcaa aaataggcta taaagcacag cttatccagc ccgttgatat gttcccgcat
     3301 accacaggta tcgaaaatgt ggtgctttta aaaagaaaaa tgatattgct cacaccaaac
     3361 agaacacctg ataaaaaggg ggataaaaaa attgaacgaa aaggtaaaac ttctcacaga
     3421 taatatcgaa aaagttatcg tcggcaagac cgactctgtg cttaaaataa tcacagccat
     3481 gctttgcggc ggtcatgttc ttatcgagga cgtgcctgga gtaggcaaaa ctcagcttgt
     3541 atcagccctt gccaggtcag tggacggcaa atataaccgt atccagctta cccctgacgt
     3601 tatgccgtcc gatatcgttg gcttttctat gatagaccag aaaactcatg agcttgagta
     3661 taaagagggt gtggcaatgt gcaatttcct cttggcagac gaaataaaca gagcctcccc
     3721 aaaatctcag tcaagcttgc ttgaagtcat ggaagaacat cagatatcca ttgacggtaa
     3781 aactcatgaa cttccattgc catttatggt gcttgccaca caaaaccctg tggaaactta
     3841 cggcacttat catttgcctg aagcccaaat ggatagattt cttatgaaga tatcaatggg
     3901 ctatccttca tatgatgatg agcttgatat catgtcaaga gccgaaagat caggctttgc
     3961 aaagaatatc cagcctgtca tgacccttga aaacgtccgt gagcttatgg gctacgctga
     4021 aaatgtaacc gcagagcttt ccgtgagaaa atatatcctg agccttgtta ccgcaacaag
     4081 aaattcagat tatgtaaagc ttggcgtttc tccccgtgga agcatcgcac tgctcaaagc
     4141 ttcaaaagcc tatgcctttg ttcagggcag aggatttgtc atgccagatg atgtcaaggc
     4201 tgttatgcct gacgttcttg cacatagact tatgctttct cctaaaggca aatccgcctt
     4261 tgaaaatgaa agggaagctc ttgaaaatat cgctcttaca gtaaaatcac ctgacgcaat
     4321 agagaaatag aggatatgac cccatgaaaa atcttttagg ttatcttacc tgcgtggcgg
     4381 tggcgatact gcttagagtt tatataaacg gaagcggtgg tacgcttgtg ctttatcttt
     4441 tagctatagc ctttgtgata tctcttgcgg cacttctcat taccaagcgt aaacttgtgg
     4501 cagccataaa gctttccaca gaaataagtg caaagaacga ggagtttgac gctgacataa
     4561 tgcttttaaa tgcaatacgc attattccct catgcgttgt tgaggtaacg gcagagtgta
     4621 cagataatat cggtgctgta agcccacttg aatttaaaac tatcgtggca ggcggcagac
     4681 agcaaagcgt cagcgttaag cttaaaacac gctatgcagg tcttggcaca gtgaaaataa
     4741 aaagcataca tttgcatgac tatcttggaa taatcactct gcaccttgac agttcacgtt
     4801 atgaaaaagc cgtgggaaaa ataaagataa tgccgaatat ccctgacact ggctctcaaa
     4861 cagagcttct taaagcaact tccgaaaacg cttcatttga cgataacgaa agcgaatcag
     4921 atgaaaatgc acttggtctt acaggcgttc cgggctatga ccacagacag tatgagatag
     4981 gcgacccgct gaaaagaata aactggaagc tttcttca
//
LOCUS       DAWEMR010000183         4995 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6326_length_4995_cov_7.265385, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000183 DAWEMR010000000
VERSION     DAWEMR010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6326_length_4995_cov_7.265385"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2304..2313
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1835 a          685 c         1111 g         1354 t
ORIGIN      
        1 gtatattgtg agggtgaaaa atgatatcat atcttgctga ttttgctagc cgtatggaac
       61 atatctcaga gatagcttcc gtgatttgta gaagaaatag aaatcttgct atagaagcaa
      121 gctttggtga taatgaactt gataatatta tatttgcagt gcttgttcat attatggaac
      181 gatcacttac cacaaacgtt cgttgtacac ttgaagatat agaagatttt cttgcagaat
      241 tatttaagga ctataatagg gaaataaatg aaagtgaatt gcgagagttg acaagataca
      301 ttgtcaagga catacttcaa aacaaagggg aaagctttaa attcaaaata atggatcata
      361 gtgatcttac gatcaaatca tattctgtta gacttataag ggacatagag gacgatgacg
      421 gcgttatcta ttatgtgctt gaaaagcagg gatttgatat gctatttcgt aacaaagagg
      481 tcgatgacct tggttttcaa ttggaggagg tccgattgca taagcttatt gagcgaggca
      541 acttttcaga tgcttcttcg caaagcaaac ggcttttagc aatgcttaat caaaaggaga
      601 tagaccttac tctttttgaa cgaagaatga aaaatgatct tagtgaaact tcaggagaag
      661 aatatgatac tcttattcat gaaatagagc agatgctcga tgacgagtat gccgttatga
      721 gtgacattaa gaaaactatt ggaaaagcga tagaaagctt ttcggatagt aatgctttct
      781 cggatagagt aaaaaatgag ttagcggaca agaagcgtaa tatttttatg atctcaaata
      841 atactgaaaa aatacttgta aaacaacgtg aacttttaag tcacagcaga gaactaggac
      901 agttgtacag agaaatgctc ttgaaaacca taacaatgca caggacgcat tgttatgata
      961 tagaagatgt tattttaaag cttctggagg agtataaagg caataataag ctggatatag
     1021 aagaactatg tgctaagttg atcgaacctc ttgtgctgcc aaaacttcca gctatgttcg
     1081 atctaagact tgtttatgag ccacagcaat caagtgaaga agtaaacgga gagatcgaag
     1141 aggatgagga tagtcttgaa gaagtttcac cttacacaaa ggaagttgaa aggaagaata
     1201 aagctaatac cgagataata aggcttatgt ttgagtttgc atcacaaaga aaagtattca
     1261 gcctgtcaga cctttgggaa tactgtcttg aaaatgcgga tataagtctt attgccaggg
     1321 ataagtgctt ttttcttatc atgctggacc tttattcaac tgaaatgatt gatactgaaa
     1381 aatgggaaaa tgaagagaac aaaacggagg atagccaagg cgaatttgac ctgtcctatt
     1441 gtttgctcaa gataagttct cataacgaaa atttctatgg ggtaaagcgt atccttataa
     1501 cccgttcagg tgagaggaca gaattgaaat gtgaatatac agacgaagaa acgggactga
     1561 gatgtaagga aaccgttcag atcgacaata tgtgttttgc agcggaggtt atgtgatatg
     1621 cgtgaaagca tgagaatttt aaaacacctt ataataaaca attctataaa tgatcgtgat
     1681 gatgctgagt tgtttgatct ggcatctcgt gaggatataa gagaagaact tgatgtaatg
     1741 agtgaagagt gggatttcac ccttgtcagg acattccatg atatttatat cgttccactg
     1801 ccagataatg aaatgttcaa tatgcgtttt agggatttca gagaaagtat tgcaacaaat
     1861 gccaaaaagg tagaggctta tttgcaatgt tatattatca tggttattct ttggatgttt
     1921 tttggaggta gaaatagtga tccacagttg acaaattcgc ttcgtataag agatattgtt
     1981 ttgaagcttg atgaaagatt ttctgatgaa caaaaggctg caaaaaagga ggaagcacag
     2041 atcgattttc ataagatagc tgataagtgg aacgggaatg tgtataaagg tgaaggtcga
     2101 aagcaaagca agatcgagct tgttaagaca gcttgcagga tacttgtgag aaacgaactt
     2161 gtgatctttg ctgatgatga agatgagata agatgcagaa aaaggttgtc agaccttatg
     2221 aaaaattatt atctgagtga taaaagaata agtgaaataa acaggctctt tgaaaaggag
     2281 gacgaaaatg ccaacgatca atcnnnnnnn nnnagttaat attttttatg acggacgaat
     2341 aataaaggac agtatctttg attattatgg cggaagaaac gccttgatga atcttaataa
     2401 tggcggcggt aaaaccgtca tgatcgagac gatatttcag ccgatcattc caggtatgga
     2461 tatcgatggt tggaagataa ctgactatct tacaggagat cagaaaccct catatgtgat
     2521 gatagaatgg atgcttgatg gaacaaagaa gccgtcctat tttatgacag gcatttgttt
     2581 gagcaaaact aacgttaggg gtgatgatga taaaaatatc aaggtgctga aatattttac
     2641 ttttgttcat gactatgatc agggcaacga atttgatatc aaaaatatca atgtttcaga
     2701 agaacgtgac ggaaagaatg tattttatac ctataatgag ttttatgaaa ggctcaaaaa
     2761 atataaaaaa agtcatgaca aatttaatgt gtatagaaaa gaaaaaaagc aagattatcg
     2821 tgaatatctt aagaaacatc atatttatga gaatgaatgg gcattgcttg caaaagttaa
     2881 tcaggatgaa ggcgataagg gattagccgg actgtttgat aagtgcaaga cttccgatga
     2941 attatttgat caatggatac taaagacagt gagtgatatg aataaggatc agcgtcaaag
     3001 ccttatcaaa gaaactgcgt cacttataaa acctatgata gaaggtggca aggctcttgc
     3061 ggagaaagag ctcttagaaa atgtgtcggg tgatcttgaa acgtttagcg atatgtttaa
     3121 agaatatacc gaagaacttg acaaaaaaga ggagaaagaa aaatatatag caggaacact
     3181 aattaactta aaaagctgta tagaagcaga agaacgtaaa tcggccgagg ctgctgagat
     3241 cataaagaaa tgcaaagaaa tagagaaaaa gataaaacat gaggagcttt cagaacagta
     3301 tcatggcatt aaaagatcac ttgatgaatc agaggataag ctaaaagaat atgatactat
     3361 tttgcctgac ctttcaaaaa tccataaaga ggctgaatat aaaagagatt gtatgcatgc
     3421 cgctgaatat tatgaaaagg tcaaagccgc agaaagtaag ctagttgcag cccgaaatac
     3481 tatagctaca ttgggaaaag gtgagatcgc tgttagaaaa aactgtcttg gcgcaactat
     3541 ttatcatcat tattcgttgt tggcaaacag tctgaaatca gaacaagaac gcttaaaaag
     3601 ggagtttgaa agtaaaggta atgagataaa tgtacttaat aacaggctcg gtgaatttga
     3661 tagtaagctt tcaaaattga atattgataa aggcagatta caggagcgct gtgacaactt
     3721 taattcagct gagcaggaat atagagagca gatcggtgaa cttcctgaca gagatcttac
     3781 gggtaatttg gtggctgaga gtatcgacga gttaggaaat catattgttt cagctattaa
     3841 aagtgcagaa acagaacagg aaaggcttta cgatataaag aatgatcttg aaaataagcg
     3901 taatgcaaac gctcaggaac tagaacagat ctcgtctgac ataagcaaga agggtcagca
     3961 agtatatgac gcacaaaaca aatatgatgc gctgaaaaat gcagaagcag tagttgctga
     4021 aattttgagc agatacgata tttcaggcac tcatttatat gaaagtgaag aaaatcttgc
     4081 atctttaaaa gagaaatatg ataatattga agccatttat caaaaggaca taaaggagag
     4141 agatcgccta aaagatactt ttgatgacct tgttcacgga aagctgcata catctcctga
     4201 atttgcagac cttcttattc aatacgacat tgaatttcag actggtgaga gctatttgaa
     4261 gcgacaggag ggcgattatt ttgatacact gcttaacaaa aatccgcttc tgccgttttg
     4321 ttttatcgta aatgataaag attatgatag gactttggag atatctcagg actttggagt
     4381 tgacagagtg tgtcctgtta ttcgcagaaa atcattggac atgaacttgg aagcttcaga
     4441 acgttccgct gagatcggtg agatcaagtt ttacagcttt tataacaaag acagtctgca
     4501 cccaatgaca agagaaaggt ataaaaataa ccttgaagat aagatatctg aactcgacga
     4561 gaagatatct cagaggaaaa atgggcttag taaaatatct gatgatatgc ggggcataga
     4621 gtcatttgga tattcaaaat cggatagcag agaaatagaa aagtctttag atgatgctaa
     4681 ggctgatctg aagaagcttg aaagtcgtag agaaactctt agaggaaaca aggatcacct
     4741 tgcaaacgaa cttgaagata caaacgatgc aataagtgag aatgaaaaat tacttattac
     4801 ctgccaaaga agagatgata agtatcatga gtatcttaag gcggcggcaa aggacgctga
     4861 aaatagagtt gctcttttaa aagcattgag tgatatcgaa gcggttagca attctatcaa
     4921 ggataataaa gaaaaagtac atctgttatc agaaagtaaa aataaattag agattaatat
     4981 agaaactatc aaacc
//
LOCUS       DAWEMR010000184         4984 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6338_length_4984_cov_11.850071, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000184 DAWEMR010000000
VERSION     DAWEMR010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6338_length_4984_cov_11.850071"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1297 a         1200 c         1002 g         1485 t
ORIGIN      
        1 cctacgaaat tagaaaggaa aaacctatgg aacttcacgc tttgggacta aactacagcc
       61 atgacgggtc ttttgatata cgccgtccaa acggttcggg ggataatctt ctgcttatat
      121 tcaaatcctc tgcggtggtg cgtttgcacg gcagggaata ctccgtttcc cctgacagcg
      181 caatactata caaaaaaggc actgagcaga tataccgtgc ctgcaagggt ttttatataa
      241 accactggct tcattttgat tgcgacgata tgctcacccg tgactattca ctcccatatg
      301 atacgatttt tatgttatca gacgcagaga aaactgagga tatcttgtcc accataagca
      361 gactcagctt atctgaaagc ggcaggcgtg aggattatat cgagcttatg ctcagaatgc
      421 tgctcataag acttggagag gaatattctc ttagcaagag cggtcagccg ccaagaaagc
      481 gcagtcacta cgctgacgca cttgagcggc tcagggctga catctacagc tctccctccg
      541 ggagcaggaa cattgccgat atggcgaaaa ctctcagcgt aagcccctcc cactttcagt
      601 atctttacac ctcacgcttc ggtgtgagct gttatgagga tattctctgt gcaagaatga
      661 aaaacgctga atactatctt ctcagcactg acatgacagt gaaaaacatt gccgccgtat
      721 gcggatacga aaatgacgtg catttcatgc ggcagttcaa aaggcgatac aacatgaccc
      781 cctcacagtt tcggcagaaa aacaaaaaat aggcatatgc aaaaagcgga cgaaaagaac
      841 tcgtccgctt ttgttctgtc aaaattctgt taaaagtcat gcgaccaaag tccgccccta
      901 caaaacagct taatcttcga tctccgactg atcctcaatg acaacagggt ggccgtcctc
      961 ggtcattatc atatcccctg cactgcactg aacagccgtt tgctctgcgg ttatgtcatt
     1021 gggtttgagt ttgaacctta tcgcctgctt gcagttttct atctcaacat atgtatgctt
     1081 tccgcccgac tctccgtcca gcgtccaagg cacttcgttc acgcttgtga atgtgacctt
     1141 tgaggtcttg aacgtcacaa aatgcttatc gttgagctta ttaagcactg cttcatttat
     1201 ggtgttgctc agctcgatag cgttcttagg ggtctttacc aagaccactt caaacagtcc
     1261 gtcatcaaag cacacacctg atgcgataag ctttttcatt ccgcccactg agatagtatt
     1321 tgagataaga ccgagtataa agctaccttc tactacctcg ccgtcatgct ccactctcat
     1381 ttgctgacct gttatctttg aaagcctttt tatcccctct gccacatatg cggcatgacc
     1441 aaacttgttt ttcatcgttt gtgaggtttc ataagaaacg tcagtgaagg cgccaaagcc
     1501 tgctacatac acaaaatact gaccgttgaa cttaccaacg tcataatcaa aatacacgcc
     1561 gtcaactatc tcccttgcgg cgtcaagcat ttttttcggt atgctcaaag actgcgcaca
     1621 atcatttgtt gaaccggcag gaatatatcc aagcggtatt ttcttatcaa atgtcatcat
     1681 tgccttgaca gcctcagaca atgttccgtc accgcctgat gtgactatca tgtcataatc
     1741 ctgaccgcac ttgcaaagct tgttatagcc gtcaagctta gcctgcgtag ggtatgtgac
     1801 tacctcatag ccgccctttg taaaaatatc cacaatgtcg caaaggttat cttttatctg
     1861 tcccttgcca gcatgggggt tgaacacaaa aagcaatttt ttcattttcc cagccccttt
     1921 tcgtttttca aattcctatt attctacttt ttatctatct ttcttacaca ctttttacat
     1981 agagaattat accacaaatt tcacaaaagt caagtattat tgcgacaacc gtcaaaaagt
     2041 cgtcaacaat cacaaacaaa ttgtcagcca aaccacgaag gtgacaaaga taataagggc
     2101 aaaacatata aaataagcaa tatggataaa cgggtgatcg ctgttcgccc ctacatatag
     2161 aaatcaagat catcgccgca ggcgatacct taattcctca ttcctcactc ctcattcctc
     2221 actaaaaaat cagtctttcg cctcaaacca gcttctgccg ctgtttacgt ccactgtcat
     2281 tggcacagca agctttacag cgttctccat ttcatctttg agtatctttt cggctctatc
     2341 cttgtctttt tcagccgctt ctataataag ctcgtcatgc acctgcaaaa taagtcttgc
     2401 gtcaagatct tcttctctaa gctttttgta tactcttacc attgctatct ttatgatatc
     2461 agccgcagca ccttggatag gtgcgttcat tgccgccctc tttccaaaag cctgcaagac
     2521 cttgtttgaa gcagaaagct cagggatata tcttcttctg ccgaaaaggg tggtgacata
     2581 gccgtttttt atgccgtaat caactgtctt atccataaat tcagatactt tcgggaagtt
     2641 gtcaagataa ttcttgatgt atctttttgc ttccgcaacg gtcacgccga tatctttcga
     2701 caaagaaaat gctcctatgc catagattat accaaagttt acagcctttg ccgcacttct
     2761 catttcaggg gtgacaaaat cctcggggat atcaaagacc tgtgccgccg ttgaggtatg
     2821 gatatctcta ccctctgaga aagcttcctg catatttttg tcaccgcaca cggaagcaag
     2881 cactctaagc tcgatctggc tgtaatcagc gtcaagaaga gtatagccgt cctcggcaac
     2941 aaagaattta cgcatattcc tgcctatctc ttttctcaca gggatatttt gcatattagg
     3001 ctctgtggag gatatcctgc ctgttcgggt ttctgtctgc ttgaaaacag aatgaactct
     3061 tccgtcaggg tggataagct tcaaaagtcc gtcaacatag gtggaattaa gctttgtgag
     3121 tgtgcggtat tcaacgataa gaggcacgat agggtgcttg tccataaggc tttcaagaac
     3181 gtccgcattg gtggaatagc ccgactttgt tttcttcttt gcaggcagac ctaagtcctc
     3241 gaaaagcacc tccccaagct gttttgtgga agctatattg aactccttgc ctgcatacat
     3301 atatatctgc gaggtaagct cgtctatctt tattttcaag tcctcaccga aagcttttat
     3361 gccctctgcg tcagccttta caccgtaaaa ctccattgaa gcaagcactt cgcaaagtgg
     3421 catttcgata tcgtcgaata gctttgtcat ttctgtcatt tcaagctgtt ttttcagatt
     3481 ttcactaaga gcaggcaggc ttgaaaggtc tgcatattcg cccatatcag agcggtaaag
     3541 gcacttatat gaaagacaaa gattttccgc tgtatactcg cttgactgtg aattgagaag
     3601 atatcctgca aggtcgcagc agaaagttag gttttcaagg tgagtgccat ttgagaacgc
     3661 aaacttatgg gcagttttgc cctcaaaggt tattttttcg caggctgttc ccatgaaagc
     3721 tgtgataatt gcattgtcct cggtggtgta gataacatcg ttatagagga tagaaagttt
     3781 attgtcggca aagataaaat acactgtttt gtccttgtca agaacagata taacagtttc
     3841 atcaagtggc ttgacctcaa acttaggcag gtcggaaagc tttagcttgc tttcctgcac
     3901 tgcgctgtca gcactttcca cagcttttcc gctaaggccc aatctgtcta tgaggcgcat
     3961 catttcaagg ttggcaagga gagcctttgt tttctcggta tcaacctcgc ccagcttata
     4021 aaactctgta gttttctcaa caggggcttc aagacatatt tccgcaagga agcggctgtc
     4081 tatagcgtcc tgatgaccat tttcaagctt tgtgcgcaca gacggggtaa gcttgatagc
     4141 tgcgaggtgt tcataaaggg tcttgacatc gccctcctgc ttgatgaggg aaagggcggt
     4201 cttttcgcct atgcccttta cgcctgaaat attgtctgag gtatcgccca taagagcctt
     4261 tacctctatc atctgacgag gtgtgacgcc gtattcttcc attatcctgt caggggtata
     4321 tatctttgtt tcctttgtgg tggcaagacg cactgtcaca cggtcggaaa cgagctgaaa
     4381 gctgtccctg tcaccagtga ggatatagca ctcgttgcct gaatctgcgg cggcttttga
     4441 gagcgtacca agaatatcat ctgcttcata tccctcgcac tgtactatct ttataccaag
     4501 gtcgcccaaa agctctttta tgacaggcat ttgctgagca agctcctcag gcataccctt
     4561 tcggttggct ttgtatgcgg cattcgcctt atgtctgaaa gtaggggctt taaggtcgaa
     4621 agccactgcc acgcagtcag gctttacctc gcctatctcc ttgaggtaaa tattcataaa
     4681 ccccgtaagg gcattcgtga actgtccctt tgaatttgac agcagcttta ttccgtaaaa
     4741 agccctgttc attatgctgt ttccgtctat ggctagtagt ttcattctag tccaagcacc
     4801 tccgctttat tgttctcatt tcttggatta tactcgtcaa cgtcccactt gatagggtta
     4861 ttgtgtatgt attttgaaat tgctatatat tctttttggt ttctgatgat atggtcataa
     4921 tatgaacgct gaaaaatatt aaaaccaata tccttattta taaatctttt caacattgac
     4981 acat
//
LOCUS       DAWEMR010000185         4970 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6354_length_4970_cov_10.369278, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000185 DAWEMR010000000
VERSION     DAWEMR010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6354_length_4970_cov_10.369278"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1378 a         1158 c         1005 g         1429 t
ORIGIN      
        1 cgccggactg aggctagtcg ccacacctgg tcacaaattc aactgcgaca atctgccacc
       61 gctgtctaag ggcgatattc tgctccacgg tcacacccat gtaccggtaa taaaagatca
      121 gggcgattac acctacataa atccgggttc agtatctatc ccgaaagaaa attcagcaca
      181 cagctatctg atactggaaa acggcaagtt ctcgtttggc gagcttatat aaagaaaagg
      241 cagcgttgag ctgccttttt tttgtgctta ttttattgta gtttcaagcc cgttgacctc
      301 tatctcaggg tcatcaagat tgattataag gcattttctg ccatcgacta tctgagtttt
      361 taccttgtca actgcgtctt tgccaatatt tacggttatg cttggagcag aaactacggt
      421 ccttttctcc acaagattta ccgctgtgag tggcttcccg ccagccgcag cgtcaaaaac
      481 tttaggaaga ttatcaagct tatcctgact aactccagct tcccacagaa tggacgaaag
      541 tttatgttcg tcaattgtcg gtatctcagt ttcatgagca ttttggtcaa caaatgtgga
      601 aatcttgtca ttcacagcag tgataagatc atagtcaagc tcatcgccca caacgcttgt
      661 gaggatatcc ttgaaagttt ccttttcgtt ctgacaggtc attgagaact cacaacccag
      721 aagctcttcc acaacggaag tgttcggctt tttggcgttt ttcgtgtagt aaagcactcc
      781 gttgatatct ggtgcacggt cattgaaaag agggaacagg aagccgtcac ttggcagttc
      841 aacaattctg tcacatgact ctttcttagc gatagagttg tcctgttcat catacacaag
      901 cccgtcaata cgcagattta cagggcaaag cgccgtcact atgaagttgt aatcagtgtc
      961 agcttcgtcc tcaaactcgt ccattttgtt ctttttcagc acagaatatg tacaatgtgc
     1021 catgaaaatg gtatatgttg acacatactc caccttttca actatggcgt tgagaaaatt
     1081 atcaaccttt tcctcatcaa gcagcttgct ttgcaatgtt tcatacatga aaggctgtgc
     1141 gccaccctca agatatgcgt ccttcggaaa cgaatattcc agcagatttt tgccgataga
     1201 accgctgagt acctttttca ggtttatcat tatcagctcc gcctcgtcct gcggaattgt
     1261 gttgtaaagc tgattggtct tgcactttat gttcttttct gcatccacaa atgccgtaac
     1321 aacgtggttt acggtaaaaa agccgcagtc atcgctgaaa ttcttcttaa tttcatttat
     1381 ttcctttttg ttcatcttgg cgtcctcccg aaaaaatgta tattaattat agcacaaatg
     1441 tacagatttt tcaagagtcg taaacaaaaa aatcccacag ccgaaactgt gggataattg
     1501 ttattaaact gggtgaacct tattttcagc gtctgagtgc ttaccgtcaa cgccgtactt
     1561 tctatcacgt ctgtccttga agaacttagg tgcaacctta gggaacattg catatgtgag
     1621 gatatcttcc tcctgctctg tccactcagc agcctctttc ttcattgttt caaactctgg
     1681 ctggagaagg tcagcaggac ggcaagtgat aggctcttcg tcgccaagaa tcttcttctg
     1741 gaactcaggg tcgatagcaa ctggtgtctt accgtactca cccttaacaa gtcccttgaa
     1801 ctccttagtt actgtcttgt atctctcacc catgattacg ttaaacacag cctgtgtacc
     1861 aacgatctgt gatgtaggtg taacaagtgg tggataacct gcgtccttac gaacctttgg
     1921 aacttccttg agaacgtcgt caagctgatc tgccttgcca gcctgtttca gctgtgaaag
     1981 gaggtttgaa agcattccgc cagggacctg atacagaagt gcatttgtat ttgtagcgag
     2041 cattgaaggg tcaagcagac cattgtcgat gtacttcttt ctgagtttca tgaagtaatc
     2101 tcttatcacg ttgagcttaa caaggtcaag acctgtgtca tactcagtgc ccttgaatgt
     2161 agcaactata gactcagttg gtgcatgaga tgtaccaaga gcaagtggtg acattgctgt
     2221 atcaacgccg tcaacgccag cctcaactgc cttgaggata cacatagatg caagacctga
     2281 tgtatagtgt gtatgcagct ggataggaat tttaactgct gacttgaggt ccttaacaag
     2341 tgtttcagcc tcatatggag tcagaagagc cgccatatcc ttgatacaga tagaatctgc
     2401 acctgcattc tcgatctgct ttgcatagtt catgtaatac tcatgagtaa atacctcgcc
     2461 aagtgtgtac gagatagcaa cctgagcgtg accattttcc ttcttagcag ccttgatagc
     2521 tgtttcaagg tttctgatat cgttcagtgc gtcaaaaatt ctgatgatat caataccatt
     2581 tgcaatagac ttctgtacaa aatactcaac aaggtcatca gcatagtgac ggtagccaag
     2641 catattctga cctctgaaaa gcatctggag ctttgtgtta ggcatttctt ttctaagtat
     2701 acgaagtctt tcccatggat cttcatttaa gaatctgata catgagtcaa atgtagcacc
     2761 gccccaacac tcagcagaat aaaaaccgat cttgtcgatc tctggaagta taggacgcat
     2821 atcgtccatt gacattcttg tagcaagaag tgactgatga gcgtcacgga ggacagtttc
     2881 agttatttta agctttgcca ttttaaatcg atcctttcgt tatttttcag gaatgttatt
     2941 agccgataac agcaagaact gcaccagtct caacagcaga gcccttagtt gtattaacgc
     3001 tcagtacctt accgtcgcaa ggagcgttaa cgtcattttc cattttcata gcctcaagta
     3061 cgcaaatagc ctgaccagac ttaacagtgt cgcccacagc taccttgatg tcaaggatag
     3121 taccaggcat tggagctgtt acctttgtgc catccgcaac aggtgcagcg gctggagctg
     3181 gagcagcagg agcggcagca acaggggctg cggctacagg agcagctgcg gcacttccgc
     3241 cgacttcttc aactgcaacg tcataagctt ttccgtttac tgtaatatta tatcttttca
     3301 tcttaaaaaa ccaccaatct ttttaatttg atatgtttat cacaactgaa tattgctgtg
     3361 tttcttaggc agtctggaaa ttctctttcc agccattacc tcgatagagt ccataagtgt
     3421 ctgtctaatc tcgttcttag cgatgatacc gtcaacgcag ccattctttg cagcaacagc
     3481 agcagaggca ttttccttag cgaacttatc agcaagagtg tttcttgcag cagtaagatc
     3541 agcagcaccc ttaagctcgt catgctgaag gaattcaaca gcagtaacag gagcaagtgc
     3601 agagataaca gcggtatcaa gagcaaatga cagatcagca tttgaaccct tgccggcaag
     3661 tgcaataaat gcaggaccat aagccttacc tgtaacaaca gcaagtttaa tagtagtagc
     3721 ctcagcgtaa gcgtggcaaa gctttgccat atccttaaca gcgccgtaag cttcagtttc
     3781 atcatcagct ttgaagccct cagtatcaac aaaagttaca acagggatag cgtaagcgtc
     3841 acaagttctt acaaatcttg cgatctttga gcaatcagca gatgtaagct tgtcgcctgt
     3901 cttgttagta gcaacaacgc cgactgtagc accgccaagt gttgcaagtg ctgtataaga
     3961 agccttgcca tactcagcat taagctcagt tacactgtcg ccatcgcaaa cagcctttgc
     4021 aatgccatca gcgtcatcac caacagcctc agttggatca gcaaattcat acattggtac
     4081 tggtgaaagg ttgttctgtg gaagttttgt aagtatattc tttgcagcct caacagcagc
     4141 cttatcgtcc tcacaaacaa cagcagcagt gccgttttca gcagcatttt cagcagagtt
     4201 ctttatgttt gcgtttggtg caacataaag ctcagcatcc tttgcgataa cagtgaagtc
     4261 agcagactca gcgagcattg ctgcacagcc tgcacaagtg ccagcgacaa cagctatctg
     4321 aggaacaaca cctgaaaggt tagaagtcca cataagcagt tcaccataag cattgagagc
     4381 ctttgaaccg tcgcttacgt cagcaccata agaatcgaat attccgacaa caggaactcc
     4441 tgtcttagca gcaaggtcat aaacctttgc tatcttcttt gcctgtgcct ctgtcaaagc
     4501 accgtcctta acagtcacgt cctgagaaaa agcatataca ggattaccct caacatagcc
     4561 aaaagcagta ataactccag caagggagtc acccgcagca gcataagcgt caagctctgt
     4621 aaattttcct tcgtcaaaca aatatgtcag cctgtttctt gcagcagtag gagtctgcga
     4681 cgcagcttta agctcagcaa gagtagaaat tgaatcagac atacagtttc ctccattcaa
     4741 ataatttttg tgataaaata acgcaaaact actggatttt atcatattta tacacttaat
     4801 attatactat ttttttgccg aatttacaag agtaaattga cctatttccg catattttac
     4861 gtttagtagc ataatcaata tcgatacacg catattatta agcattttgc acaatagcaa
     4921 agggtctcct gacaaaaaga gaccctcgat aagcgttatt ttttggtgat
//
LOCUS       DAWEMR010000186         4938 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6395_length_4938_cov_10.843948, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000186 DAWEMR010000000
VERSION     DAWEMR010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6395_length_4938_cov_10.843948"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3731..3740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1456 a         1040 c          824 g         1608 t
ORIGIN      
        1 ctgtgaatca agaacaagga cagtatcaat ctcaaactga aattttacca gctcgttatt
       61 aacatacaat gcatttttat gtatttttaa aataggcata gttaacctcc gatatcataa
      121 taatcgttcg tttgtgaact taccaataaa ctgacccatt aaatcatatt gagttccgcc
      181 gccgttcccc caatttatta ttttattggc gattccataa cgaacagtac ttcctgcagg
      241 aatttcaaca tcacaaatat atttaggttc atactccaaa gcaaatttgt tttttatctg
      301 tgtcggtgtt agaccttcaa tttgctcagc ttccattacc caacctccgg ttagttttcc
      361 agactctcga taaaccctaa caaattttgt gttttcttta agtttaacgg ttatggtctt
      421 tgtcccaggt tgatagggtg gtttgtagtt agaattggtt ttaaattcgc tgtttacttt
      481 ttcagctgat tctattttga caatatcgcc tttttgagat attttagaaa tatcatctaa
      541 tgcagcttgc tgttttttag ataattcaaa agaattaaca gctttggtgt tcttagcctg
      601 aaaagcattt tctaacaccc aagtagataa gaaaaactga aaagattgtg tccaacttgt
      661 ataacctatg ctcattatct gagaccaaga agtttcgtct ctataattaa aatattctgt
      721 ataataaatt tggaatgcag ctttatcgca tttataccct aatgacgcta aatcctcaat
      781 cacttcgtca atcgcataat cagcatgtct gcgataaaca tttatgtaat ctatcgcaat
      841 aattggggag cattcgaatc tttcacaaaa ttgtgccaaa tactgtattt caaaaagttc
      901 agctgaatca aaaaagtcaa taatttttaa ttggtcatca gttgcaggat gaatcgggtc
      961 aaaatcgctg tcatcatcta acccatctcc atcagtatcc tcattccaag gatccgagtt
     1021 gtatctgaac actaatgaag aatattccgc atggccgcct gtagaatcct ttttgattac
     1081 aaacttagga ttttcgattg aaatttcttc tccatcaagc aatccgtccc cgtcacaatc
     1141 cggattttca ggatcggtat agaaaatctg tgcattagaa acaaccaatc ccgaaacctc
     1201 aaattcatct ggaagaccgt cgccgtccag atcctccatg ttctgaagat caaaatgatt
     1261 atctcttgac aaatcgttat aaatagcttc aagctcctcg gcagtcgctg ccgcaaagaa
     1321 ttgtcctccg gtagactcgg catatgattt cagcctgctg ttgcttgcac cgtaacctaa
     1381 accaatcgta tgtattttta tctttttatc ctgcgccttt tgtatatact cctctgatat
     1441 atttacatct ccgtcagaca gaagaataat atgtctggta taccaatgtt caacgctttc
     1501 caattcatca atggcgatct taacggcagc ctgtgcattt gtgccacctg aattatatgg
     1561 ctttactgca ctctttagca tatccttatc atttgaaaaa ccgcttacaa gcttagcaga
     1621 gctttcataa ctgatcagag ccgttttgtc agtcaatgtc attgaatcta tataattatc
     1681 tattgcaagc tttctgtagc aggtaggata agaataagct atcgtcccgt cctcgttata
     1741 tatattataa ttaaaggtac ggtcattgct actcatactt cccgaacagt cgtaacaaat
     1801 cacggtagca agcggttcgt aggtatcttc aaaataaagt gaagcttttt tccaagcatt
     1861 gaaccactct actttgtcca ccagcatata cttactgaag tgtgtagtct ccacgcttac
     1921 agttgaatta tcctcatcaa gcacagtgtc cagctcaaca aaattgtcat tttcttcatc
     1981 ataccataaa aacagcagat tgtcaaattc ggtatcacca agcttagact tatcgatcac
     2041 ataagtaagc gtagccttat caaactgcga agtcgttttg atttcaaaag gctcgcccac
     2101 aagacctaca acctctgagc agagtatatc cttgttcata atgctttcaa cagtagtggc
     2161 tttctggaga ttgcccgtac cctccataga cacacgaact tcggtaacgg cacaatcctc
     2221 gttttcaacc ttgtgaataa aggtctggaa acgcttttca tcaccgtcaa gaactccgtt
     2281 gccatcggta tcgggattca gcggatctgt ttcaaagtag atctcgtcac cgtcctcaag
     2341 tccgtcatca tcggtgtcaa ccttgagcgg atcagttatg taagtgttga tctcctcgcc
     2401 gtcattaagt ccgtcgccgt ccgtatcatc attataaggc tctgtaccaa gttcatactc
     2461 ctgaagattt gttaatccgt cttcatcaaa atcctcatcg cagtcaagga cgccgttgtc
     2521 atcggtatcg ggctttgttg gatctgttcc aagcgttaat gcctcgtatc cgtcaggaag
     2581 tccgtcacca tctgtgtcag gattgtacgg gtctgagccg atttcctttt caataaggtt
     2641 aggcaaaccg tcctcgtctg tatcggcaag gtactgccaa tcgtccattg tccagctgag
     2701 ttcttcgctc tctgtgccaa ctaattcagc ccatgaactg ctgtagttga tattagctcc
     2761 ataaccgtcg ataacaacat tctgtgcaat gataagtcca ttcatattga aattgtcgca
     2821 gtctatggta acagttccga acggagcata gatcagaccg tttacagttg cctgactgtt
     2881 tgagatatca atatcgccga attttgaata gataactgta ttatttccgt tgagatttcc
     2941 gcctgtaaga tcaacgtccg aaacagcacc cacagcgtca ttcaagctga tattgccgtc
     3001 taaattcagt ctgcctgtta catagatcgg gtcatttata ttcacattca tatcggaata
     3061 tgtgtaatcc tcgtcgtatg tatcacagcc ttcagtgaaa tacttgcttg tcagcttggt
     3121 gtgaatgagt atcatatctt tgttgacatc aaatacatct tcggtttctt actctgcgtt
     3181 gtcttcatca gtaatatcgt cagcatctgt aacagtacca ttgatattag gatattgtgc
     3241 agttgtcgag aaaactccgt ttgtgtaagc attaccgttt aaggtgaaac tgtctgtatt
     3301 gacagtaatg cctgcgtctt catcagcggc aaaaacagca taagggtact ttgctgtttt
     3361 ctcagcagat gcgggcataa ccgcagctgc acttgcggtc atagctaaag cgagtgcgcc
     3421 ggacaacatt cgtttcaatc gtgcctttgt ttttgtttta aacatttctt ttacgtccgt
     3481 tattttatta atgaaacttg ttaagttaaa acaaacctct tgttaataat tatataatat
     3541 taacaaccaa aaatcaacaa tatttataaa aaatatattt aaattctaaa tttcagaact
     3601 tacactaatt ttaagttaat taaattaaga atattgtaat ttttaaacaa atgttctaca
     3661 ttattcgatt atttacgaca ataagcgaca catttaattt aatgttttgc ataatcagtt
     3721 ttatatattt nnnnnnnnnn atgcagactg ttatctaata tgcttgaaaa tgctattact
     3781 gcaactgcaa acataaaact gaatgctaag cagataaatc tgaaaatcat ctccgagtca
     3841 gaaaaatata cttttcttat caaaaattcc attgacgagt ctgttctgaa agaaaatccg
     3901 aaactacata cgactaagac gaaatttgat tctcatgggt atggcattaa aattattcgt
     3961 gatattgcaa aaaagtataa cggtcattgc gatttttatg aagaaagcaa tatgttttgc
     4021 tgccttgttg ttttaattcc gaataaataa caaaaatgtg tatcatttaa aaatcgagtt
     4081 gtttggtaag cgtaaaataa cccgcgttta tttacagcag aaaaagacct cgccgaaaaa
     4141 cacagcgagg ttaagagcgt attagttttt ccacggcaaa agaattgtgc cattcggttt
     4201 tgagaacaac ctggtcaagt atttttcagc gggcaggtta ctcatccatt cgtcatgtgt
     4261 acgctttgcc ttgcactaat gccacacatt tctcacggag cttacgacat gttcttttcc
     4321 tcctgtgtct aatttgatcc aacacactct atagactaac atagattttc tcatccgctc
     4381 tatagagttg cttggacttt attatacacc aaagttcaat tgtttggaag tcgtggattt
     4441 attttacgct ttctttgctt ttagcctggg cctttgagaa actaaaaaag tttatgcata
     4501 atatgacaaa accctcctaa tgtctagaag ggttctatgg agctggaaac gtgacttgaa
     4561 cacgcgacct gctcattacg aatgagctgc tctaccaact gagctattcc agcatatatt
     4621 aaatttttct gttagcttat ttgatccgcc gccgtgtcgc tttatcaaca acccacgacc
     4681 tgctcattac gaatgagctg ctctaccaac tgagccatat cagcatacaa gacttaagta
     4741 ttatacacca aaaccacaca tttgtcaaca ttaattcaag atttttttca aagctttgca
     4801 aaatccatat caaatagtca tatcatatgt aacttgtgag tataatgaat gaaataaata
     4861 cgttttcttt ccacatcgtt attttgcaca ataaaaaaac gctgaaatat gcttaaacgt
     4921 ttcaaaatat cttcgcaa
//
LOCUS       DAWEMR010000187         4936 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6398_length_4936_cov_8.567712, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000187 DAWEMR010000000
VERSION     DAWEMR010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6398_length_4936_cov_8.567712"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1497 a          982 c         1011 g         1446 t
ORIGIN      
        1 tattccatta taccatattt ttttttcttt ttcaactgtt atttgaatat ataatataca
       61 taccttacat tattatttta agtgcaattt aacaaaaaaa tgactgcctg ctcccaaaag
      121 ggaacaagca gtcggaggtt tattatagat caactaataa tcaaatatta gtcactctgc
      181 aaattgcttt gtcagcaaag attatttgcc gtagtaagcc ttgaggtaca tttccttcag
      241 ctcgctcatc agtgggtatc ttgggttagc acctgtgcac tggtcatcaa atgcgtcctc
      301 aaccatgttg tcgagagtgt caaggaagtg cttctcgtct acgccgtaat cagcgatagt
      361 cttcttgata ccaactcttt ccttaagagc ctcgatagcg tcgatgaact tgttgagtgt
      421 atcctcatcg ttcttaccaa cgataccaag tgaatgtgca cactcgcagt atctctcaag
      481 agtatgtgga tactgatact gtgagaaagt acccattttt ctaggtgttg ggtcagcgtt
      541 gtatctcatt accattgtga taaggagagc gttagcaatg ccgtgtggca ggtgatgata
      601 agcaccgagc ttatgagcca ttgagtggca aacaccgagg aatgcgttag cgaatgccat
      661 accagccatt gtagatgctg tagccatttc ctcacgagcc ttaacgtctg tctgaccgtt
      721 gtcatatgca cctggcagat acttgaagat aagtttcata gctctaagtg caagaccgtc
      781 agtgtaatct gtagccatta cagatgcata agcctcaaga gcgtgtgtca gagcgtcgat
      841 acctgatgca gaagtcagac ctcttggagc agacatcatc atatctgtat caacgatagc
      901 catgttaggc atgagctggt agtcagccag aggatactta gtacctgtct tttcatcagt
      961 gataactgcg aaaggtgtta cctcagaacc tgtaccagaa gatgttggga tagcaatgaa
     1021 gtatgccttc tcacccatct tagggaatgt gtaaactctc ttacggatat cgatatatct
     1081 catagccata tcgaagaagt cagcgtctgg atgctcgtaa agaacccaca tgatcttacc
     1141 agcgtccatt gcagaaccac caccgatagc gataattgtg tcaggctcga aagctctcaa
     1201 ctgagcaaca ccctcaagtg cacaagcaag tgttgggtct ggagcaacat cgaagaatgt
     1261 ttcgtggaga atacccattt catcgagttt atctgtgata gcctttgtat aaccattctt
     1321 ataaaggaac tggtctgtta cgatgaatgc ctttttcttg cccataacag tcttcaactc
     1381 gtcaagagca acagggagac aacccttctt gaagtatacc ttttcaggtg ctctgaacca
     1441 aagcatattc tctctcctct caacgactgt cttaatgttc agcaggtgct taacgccaac
     1501 gttctcagaa actgagttgc cgccccatga gccgcagccg agtgtgaggg aaggagcaaa
     1561 gtcaaagttg tagaggtcac cgataccgcc gagtgaagaa ggtgtgttta cgataagacg
     1621 gcaagtcttc atccttgcag cgaacttatt gagcttttct ctctcgttta tgttatctac
     1681 ccaaagaact gaagtatgac caagaccgcc attgtttacg agaagtgcct ctgcgatatc
     1741 aagagcgtcg tcaaagttct ttgacttgta cataccaagg atagttgtga gcttttcgtg
     1801 accgaaagcc tcttcaggag ctgtgcttgt agcctcgccg atgagtacct ttgtatcctc
     1861 tggaacttca aagccagcca tcttagctat agttacagga cgctgaccaa cgatcttagc
     1921 gttaagagca ccgttgataa gcattgtctt tcttatcttg tcagcctctt catcattaag
     1981 gaagtagcaa cctctcttga tgaattcctt cttaacatcg ttgtaaactg tcttatcagc
     2041 gataactgtc tgctctgtag cacagatcat accgttatca aatgtcttag aatgaatgat
     2101 agagtttaca gcgtttacga tatcagcaga agaatcgatg atagctgatg cgttaccggc
     2161 accaacaccg agagctggtg taccagatga ataagcagcc tttaccattc caggaccgcc
     2221 tgtagcgagg attgtgtcag actctctcat gatcatgttt gtgagttcaa gagaaggaac
     2281 atcgatccaa ccgatgatac cctttggagc acctgctgca acagctgcat cataaacgat
     2341 cttagcagca gcgatagttg acttctttgc tcttgggtgt ggtgagatta tgataccatt
     2401 tcttgtctta agacagataa gtgtcttgaa tatagcagta gaagttgggt ttgttgttgg
     2461 gataacagca gcgataagac cgataggctc gtaaattctc attgtgccgt aagccttatt
     2521 ttcctcaaaa acaccacaag tctgaacatt cttgtactta ttgtagatgt gctcagcagc
     2581 atagttgttc ttgataacct tgtcttcaac gatacccatg cctgtttcct caacagccat
     2641 ctttgcaagt gggattcttg ccttgttagc agcgatagca gcagccttga aaatcttatc
     2701 tacctgctcc tgtgtgtagg tagcgaagat tgcctgcgca gctcttactt cagcgatctt
     2761 agcagtcaaa gcttcctctg tatcgacgat gccgactgtt ttagcttcac tctttgccat
     2821 aaaataaacc tccaataaat taattattgt tttttactct tgataacccc cgctcgggtt
     2881 ttatcttagt tatattatat acgattgtta tttttttgtc aatcttataa agttacagct
     2941 tttttgatat tttacgtcgc tttattatgc aatatacata aatattgaac attgttttat
     3001 gatagattaa caattcatga ccgtcaaatt gcacaataaa aatcagattg ataaaaattt
     3061 atcatatatg ctgcctgaaa gaagattaat aaatataaga taaaatctaa ataaaaataa
     3121 aacagttgtg aaaataacga aaaagtgctt gacatttaga aaatctgtag tataatatag
     3181 acataacaga gatacataga aagcacggag atgtaaaggt aattttacac aataaggctg
     3241 ttgaaaaatc gatgtatact ctgaagctcg acttaatacc atattttaaa ataaaaggag
     3301 atatgactta tgaaacttga taactttgta gcatccagcg acatctatga tgtttatgaa
     3361 tctgatggaa tggcagtcag gatctataag aagcctgaat acaaggaaaa gtgtctgtat
     3421 gcggctctta cacacgcaag agttgaaaca acacttggca attcctttat aaagatgcct
     3481 gttcttcacg aggtttccgt tatcgacggc aaatgggcta ttacaatgga ctttataaaa
     3541 ggtaagactc ttcagcagct tatggacgaa aatccagaca agaaagacac atatcttaac
     3601 cagtttatcg acattcagtg cgagatacac gctcagtata tgccacttct ttcaaagctg
     3661 aaagataaga tggcaagaca gatcaagact cttggtcaga tcgacgagat aaagaagtat
     3721 gagcttctca caagacttga ttcaatgcca aagcacatca agctttgcca tggtaacttt
     3781 gagccaaaga acgttatcat caatgacgaa ggtacttatg ttatcaattg gggttctgct
     3841 agacaaggca atgcttctgc tgacgttgca agaacatacc tgctgttctg ccttaacaat
     3901 cctgatcttg ctgaggctta tcttgataag tattgcgtaa agagcggaac atctaagcag
     3961 tatgtacagg cttggttgcc gatcgttgca gcagcacagc tcattaaggg caaaccagag
     4021 gagaaggatc tgctcatgaa gtggatcgac gttgtagatt acgattagtc ttttcgtcag
     4081 gttgtaacaa atttgtaacc tcttattgtg caaatagcta aaaaatgatt tatcccgatt
     4141 atgagtttcg tcataatcgg gatattttgt tacaattctt tataatctct ttacagagta
     4201 attttcatgt gatataatgt gaaactgttg aaatatgaca taatgattta ggactacaga
     4261 ggttattcgg aggatcaata tgaaaaagct aatgatagct gttgctatag tttgcactat
     4321 gtttactttc ccggcttgcg gaaaaaatgc cgataataaa acagaaaata atgttaatct
     4381 tacaaataat gacgtaacga cagctcagac agaggtttct gatgaagtgc cagacgaaac
     4441 tacttcactc cctgacagaa gcactcttga aaagatacca gatgaggctg ttgattcgtt
     4501 taatcaggtc gtagatgtct gcaaggagat atatcctttg gctgaaaaaa caaaaaggct
     4561 ttatggttat acaggcacta agactgtgaa caaaaagaat tgttacattt ttgttatata
     4621 tgataaaaat gacgatgtgt tgacaaaggt ggcaactgtc gctgccgagg tcgcatcaga
     4681 taagctttac gttcttgatg atgaaacagg aagtgctgaa aaaatcaata ccgaggatgt
     4741 taaaactcaa tctacagagt catgggcaga tcaagtaact gagtcttttg ctgaatacta
     4801 tcagagcaaa gaggttaatg accatcttgc tgcagttgtt gaagcagctg cggttaaaac
     4861 agttcgctaa tactttatat aaaaaaggcg ctgtaaaaaa acgcgcaata aaaaaagact
     4921 gcaaccaccc ttaaaa
//
LOCUS       DAWEMR010000188         4884 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6465_length_4884_cov_11.667219, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000188 DAWEMR010000000
VERSION     DAWEMR010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6465_length_4884_cov_11.667219"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1419 a          987 c          873 g         1605 t
ORIGIN      
        1 agtctttttt tattgcgcgt ttttttacag cgcctgtttt tttgattaag taaacatatt
       61 gtagtagtat tcgtaaatat ccttaacagt atcggaaatg tcatattgtg gataccagcc
      121 aagatctttt cttataagtt cattgcaaca gtatatcttg gcattttcac cattacgcac
      181 aagagaaggg tctatttcga tacttatttt gcaagacgaa agagaaacta tatagtcgag
      241 aatatctttt agttttgtgg cttttccgct gcctacgtta tatatggttt tacttttttc
      301 gctttcaagt atcattctgt aggctcttac tacgtcacgc acatcgctaa agtcacgata
      361 tacgtcaatg tttcctacaa tgatattgcc attctctttt tttgcagata atagcgccac
      421 ttgcctgcac cagcttggta taacaaatct gtcggattgt ccaacaccag tatgattaaa
      481 tgccctgaca cagtatattt tcatgccata ttgctctctg tatagttttg caaatcgttc
      541 ctgagttatt ttcgataatc catacgggct gcatgagtca agagaaaatt cttcattgat
      601 aggatcatca gactgctggt attcttcgct tgagcctatg accaatattt tggcattggg
      661 tattatctct tttgctgatt caaatatgtt cagtgttccc tctacattta cagccatagt
      721 ttttgcaggc tctttccaag aatagctgac gctgcttatt gccgcaaggt caacaatata
      781 cattggtttt gtttcagata tgacttttct tacctgaact tggtcaagaa tatcacaagg
      841 aatgaatgtt acttgatctg caatatcaga actttctgat atatcacagc catagacttt
      901 atatccaaag ctacaaaatt cttcagcaag atatccgcct acaaagcctg aaacaccaaa
      961 tatcagtact acgcctgtca tagccgcacc acctttatcg tatcattaat ttctgtgtcg
     1021 cctctttttc ggctagctgg cgatcgtgct ttgacataat agcgcaaagc tgttcatagc
     1081 ttgttttcag cggatcccag cccagaacag tgcgcgcctt ggtaggattg ccaaggaggt
     1141 ttacaacatc ggtaggtctg aaccattttg gatttacgca aacaagcatt ttaccagtct
     1201 ttgcgtcata acccttttca tcaataccat ttccttgcca ttctatcttg ataccattat
     1261 tggcaaaggc aagctcagta aattctctta cagtgtgctg aacaccagtt gctattacaa
     1321 agtcatctgg tttatcctgc tggagcatga gccacataca ttccacataa tccttagcat
     1381 atccccaatc tcttagagcg ttgagattgc ctagttcaag atgatcttgg agtccgcagg
     1441 ctattctgcc tactgccatt gttatctttc tggtaacaaa attttcacct ctacgctctg
     1501 attcgtggtt gaaaagaata ccgttgcagg caaacattcc atatgcttca cgatattgtt
     1561 ttatcatcca atagccatac tgctttgcaa ctgcatatgg gctgtatggg tgaaatggtg
     1621 ttgtttcgct ttgtggacac tcttccactt tgccgaaaag ttcagaagta gatgcctgat
     1681 aaattcggca ggatttatcc atgccgagca tatgtactgc ttcaagtatt ctcaatacgc
     1741 caagtgcgtc tacatcgcct gtgtattcgg ctgcgtcaaa ggatacctgt acatggcttt
     1801 gtgccgcaag gttgtatatt tcatcgggac gaacttcatt cactaccctg ataatgcttg
     1861 acgagtcgga aagatcgccg tcatgcagtg ttacagcgtt ttcagctata agatgatcta
     1921 ttctttcggt gtttgaaaca gaagaacggc gaacaatgcc gtgaacttca taatttttgc
     1981 taataagtag ttctgcaaga taagagccgt cttgtcctgt aattcctgtt attaaggcct
     2041 ttttcatatt atcctccatg atatttctaa aatgtttcta agaatatgtg cttgtccttt
     2101 acgctgatag actcgcctat ctgtatttca ataatgttca tatctgtctg ggcatggata
     2161 aggtgttttg caccctttga aactgaaatg gtgtctccgg tttttactaa ctttttctcg
     2221 ccgtccacaa caacatatcc gtttcctgaa actatcgtcc atatctcact gcgctgttcg
     2281 tgactgtgat atttcatgga atggttttct ttcaggtata tgcttactgt cattgaattt
     2341 agctgaacat caagcactgt aaaagtaccc catgattttt ctgcaaacat tgccatattt
     2401 cccagctttt ccacatatgg cttcatgtat ccgctggttt ccttatcaga aacaagtatg
     2461 ccgtcattgc ttgctgcaac tatcatgttt ttacagccca tacaaagaag tggaatacca
     2521 agttcattta taacgtgggt gttttcacat ttatcatcaa gaagcgcatt gcctttgaca
     2581 ttttcagaca tgacctccga catcatgttc catgttccta catctcgcca ttcgccttta
     2641 tatctcataa cgtctatgtt tgtttctttt tctgataccg catagtcaaa gcttatcttt
     2701 tcaagacttt catatttttc aagtagatca taatagtcgg aatattctat aagttcgtgg
     2761 gatttttcaa ttatgtattc aagtgaaaat gaaaacacac ctgcattcca caacgcaccc
     2821 tgctcaatat acttttcagc agatattcta tcaggctttt ccacaaactt tttaactgaa
     2881 cttatgcggt gatcattttc aggaataatg tagccgtatt tttcacttgg ataagttggc
     2941 tcaacgccta aaagcataag ctttttgctg acagtttcta tgcttttcat aagtgagata
     3001 actgctctgt agtagtcatc atgtacatat gggtcaacag gacaaacagc tatgctttca
     3061 gataatccca cgccttttat atcgtgaaga taagccgctg caagcactat cgccggaaat
     3121 gtatcacgtc tgcatggctc aatgcagata tcaacatcat caccaagctg gtttcttatg
     3181 gtgcttacct ggtcttcgcc tgttgcaata gttactccca catctggtga aacgcttttt
     3241 atctgacgat agacacgctg tgccatggat tcataggaat cattgcccgt tttgaataat
     3301 ttgagaaact gtttgcttct tatgctgtta gacagcggcc ataagcgctg tccgcttccg
     3361 cctgaaagca ggataatatt catttcatac tctttccttt attgtttatt atgatgtgct
     3421 atagcaagcg tcatttgttt cgctgcaaca ttatatgcta tatgatatgt atgcagatct
     3481 gttattgttg tgtcattgtc attacccatt aattcttcaa atgtgtaaat tgggatcgta
     3541 gaattaaatg accattcatc tgtgaatata taaaaatcgt tagtatagtc atatatgttt
     3601 gaaatgtcac caatattcac gatattgact ggaaaaacgt catatgttct aagaactgta
     3661 aatgtttgat cacaaacata atgagtggca ttaggaaagt aatatgacat ttctccaatg
     3721 gtccattcat gaagatgaag aaatgcaata tttccatctg ggttttgttt gttaagatca
     3781 gacgctattt tatccatatc ggctttattg atatcttgta tagtagaatt agctgaataa
     3841 attaaattgg tacacataac tataagaatc agtgtagata atattttgtt tccaaaacgc
     3901 cctaatagca cgcctacaag tacaagccat actgtgccta aaatataata atatcgctta
     3961 gatgcaaggt tagtaaagaa caaatttatt agtgttaaaa tcaaaaatgg tattagcaga
     4021 aataacaaaa gaagaaaaaa atctctatag actgttttct tatctgcaag ggcaaaaatt
     4081 tctttgaatg ttttggcttt ttttattgat ttcagtttga tatgtctgag caaaacggag
     4141 aataatagca aggaaaagat gagcttaaac gtaacagtaa taccaccgct gataagcatt
     4201 tgtttaaatg tccaatccaa agcatccctt gtgtcaaatt gagataccca gtaatgctct
     4261 tgcacatttg atatttgctt gaaaagcata gtcaaccatg gaatatatgc taacgcacat
     4321 ataactccgc aaataagaaa ctttttgaac ttcttgtagt tccgtgtgaa aagagaaaac
     4381 agcagtacag ccacaaatgc accgaacaca ccaacaaggg caatgttatg tgtatacatt
     4441 gcaagtatag aaaagataac agtgcataca tagccatgtt tcttttcaag gctgtaggca
     4501 taaagcatat acacagccgc acccataaag aagaacattc cgaaaatagt cggtcttatt
     4561 tcatggatct gataaaagat tggcggtgaa aaaaatgtta taagaagtgc tgcaatagct
     4621 gaatttctgc caaatatcct ttttacagga aatgcagcta taaaatacat tccgataact
     4681 gcgatgattg aaaaacagcg catcacaatg agactgttgc caaatatcac aacaaaaacc
     4741 ttgagtgcta cggcgtaaaa aggcgggctg taatcatagg gtatcaagcg ccatatttct
     4801 gaaaaagaat gttgagtaag agcatactgt gacccgcccc ctgtcaagta gacagcgcaa
     4861 aaaacaaaaa tattctatgt tgtg
//
LOCUS       DAWEMR010000189         4864 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6504_length_4864_cov_10.201289, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000189 DAWEMR010000000
VERSION     DAWEMR010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6504_length_4864_cov_10.201289"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3673..3682
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1433 a          907 c         1329 g         1185 t
ORIGIN      
        1 tagaaagata gaggtctttc agagaaagcc taactgggta aatctcaaaa gaaatatcca
       61 tgcggcagag tgtcttgtaa agtcaggaag cactgcaaag aagataataa aggaagcaag
      121 acctgatctt gttataggaa ccggaggata tgtttcagga cctatagtta gaaaagccgc
      181 taaaatgggc ataaagacgg ctattcacga gcagaacgct tttccgggcg taacaaacaa
      241 gatacttgca aagtctgcgg acgttgttat gctcactgtg aaagaggcag aaaagtatat
      301 gcccgaaaat gctaagacgg tggtgacagg tctgcctgta aggggagctt tcaacaccat
      361 gtcaaagtcg gaggctaaaa aacagcttgg ctttgatgaa aatgaaatat gtgtgctttc
      421 aacaggcgga agtctgggtg cgagagtgct taatgagaat atcgcaaagc ttttgaaatg
      481 gtatcaggac aatgatgtta aggtctgtca tatacattcc tacggcactt atcagggata
      541 taaggactat cctgaaaagc tggaggctat gggtatagat atcaacaaca gcagacgtat
      601 cataaaggat tatatcaata tgcctgtggc aatggctgcg gctgaccttg ttatatcaag
      661 gtgcggagca gcttcgctgg cggagcttga agcaatgggc agagcctctg tgcttatccc
      721 gtcgcctatg gtggctgaaa atcatcagta ttacaacggc atggtattgc aaaacgcagg
      781 agcaggcttt gttatcgaag aaaaagactt gacggaaaag cttttcattg atactgtaaa
      841 ggggcttata gaagaccctg agaagctcag acagtgctcg gaaaacgctg caaagcttca
      901 tgttgccgat actgaggaca ggatattcaa ggctttagag ccacttatta agggataagg
      961 aacgtaaaat ttaaggggat aaaataatgg ataagaatta cagacagtat aacaagaggg
     1021 cttcaatgga tagcattgaa gtgcctgatg ttgacaagat aaaaaagctg tcagataaaa
     1081 agttcaaggg cagcggagtg aaccgcaccg ccgacgagat ttttgacgga gaaaagttca
     1141 acaaggcagg ggagttcgac cgtgccacaa agacttacga tgaccctacc aagcacaagg
     1201 aagaggacga tgaggacgat ttccttgagg acgctgcggc tatgcctgcg caggcggcga
     1261 caggtcttag caagggagta aaggtgggag attcatatgt aaagcgtagc aagataataa
     1321 agacagttgt ggctgttatt atcgtgcttg tgcttatggt gttcttcttc ccaccgctgt
     1381 ttttcaacga cgcagacaag agcgtggtga gatatgacga caacgtgttc aaaacaatgg
     1441 gtatgacgga ttttaaaaca tatgcgctgt caaattattc tgtatacaac gaagaagctt
     1501 ttggttctga gttgagttca agctaccgtt ttgcagatat agtgtttaat attaaaaacc
     1561 cgtctccttt tgagataaag ataccgcagt atgagatcag ccatgtttct tctggatatg
     1621 acaacgcggt atgctatgcg accagcgtta caacaaacag cgacggtgag gtcataggcg
     1681 acactgttcc tccgttttcc agtaaggagg tcactgtaag gatacttgtg aatatcactg
     1741 atatggacga tagtacgttt gacgattgcg ttactggcat gatactttca actaaggata
     1801 tgaaaaagaa gataggcaag gactcctatc tgccttgttt gcctgctttt atgcctgttt
     1861 caaacgctac agagatatat atcaacaaat agctttaacc tatatgggcg gtcctgcata
     1921 agataagtca aaaccttaaa aggagcgacg cttatgcaac ggcttatcat tcagggagga
     1981 agcaggctga acggagagat aaccgttcag ggagcaaaaa acgcagctct gccgctgctg
     2041 gcagggtgtg ttcttataaa cggagagatc aggctacata actgtcctgc actttcggac
     2101 gtatttgcgg cttgccgcat tctgtcggtg cttggggcaa ggtgcagccg tgaggacggc
     2161 tgtgttactg tggacgcaag gggcgctgta aagacctttg tgccagatga gcttatgaga
     2221 gagatgcgtt cgtctatcgt ttttctggga gctgttcttg gaagaacagg tgaatgcagg
     2281 ctgtcattcc cgggagggtg tgagcttggt ccgagaccta tagatatgca cctgtgcgca
     2341 cttagaaaaa tgggcgtcag gataaacgag gagtatggcg tgcttagctg cacctgcccg
     2401 agagggctta gaggggctag gatagacctt tcgttcccct ctgtaggagc gactgagaac
     2461 atcatgcttg cggctgttct ggcaaagggc gacacggtga tatacaatgc ggcaagagag
     2521 cctgagatat gcgatcttgc ggagtttctg aactgctgcg gtgcaaagat aagcggcgca
     2581 gggggcggaa cgataacaat aagcggtgtg gaaaagctac acggctgtga atactctgtc
     2641 atgcccgaca ggatagttgc ggcgactttt atgagcgcgg ctgcggcaac aggcggtgag
     2701 ataggtctgc ttggggcaag ggcaggggat atccgtgcgg tcatacctat atttgagcaa
     2761 atgggctgtc aggtatttct atatgaggac aggatattta taaaagcgcc taaacagcta
     2821 agagctgtgc ggacggtgag gactatgcct tacccgggat tccctacgga cgctcaggcg
     2881 atagtcacgg cggcacttgc taaggcaaaa ggcaccagtg tggtggtgga aaatattttt
     2941 gaaaaccgct accgccatgt tgacgagctt gtgagaatgg gtgcggatat aaagacagag
     3001 ggaaaagtgg ctgtcatcga gggcgtgaag gagctttacg gagcaaaagt ccgtgcgcct
     3061 gaccttagag gcggtgcggc gttagctgtg gcggcgcttt cggcagaggg agaaacatct
     3121 ctcactaata taaagcttat ggacaggggc tatgactgca ttgaaaatgc ttttacgctt
     3181 ctgggaggaa acataaaacg tgtaaattac tagcagttag gaaagagaaa aatgaacgga
     3241 acttacaagg aaaacagaat gaactcacag cggcctgcgg cacgtcggca gacagtgaat
     3301 atgcgcaatg tgtatgttgc cagctttttt gcggctgcgg cgctgctgct ggtgctgagt
     3361 atgtttttgt tttttaatgt gtcggagata aaaatagagg gcgttaccct ttacgagcag
     3421 gatcagatcc ttggcgtggg cggcgtttca acgggacaga accttgtgag gacgaatacg
     3481 gatatagttg aaaagcgttt gaaagatact cttgtgtatc ttgatgatgt caaggtcaca
     3541 aagaaatttc cgtcaacact tgttataacc tgcacagagg ccgaaaaggc ggctgatatc
     3601 gagtataaaa attcatacta tgtgctgtct gcttcgggaa agatacttga aacgaaaaat
     3661 cctgctccga cannnnnnnn nngcttctga atgaacttca tgacctgaaa tttaaaaata
     3721 tagactctat tgacctgacc acccgttcgg atattaagct tatgtatgac ggcaggcttg
     3781 aaataaagct tggaagctcg gtggatatgg aatacaagct tacatatctt aaggcggtaa
     3841 ttgacaagag cattactgac gattacgagg gtacgcttat ttacaatggt gctgacagcg
     3901 gcatttcggc tattccgaaa tcgcaggacg agtcgtcaaa acctgacgac acaagctctg
     3961 caaagccaga tgacagcagc agtgcggtat cggctgatac taatattgat gacggcagca
     4021 catgggacag tgataactca tggtcagggg acgatagcca aggctacagc gacgatacca
     4081 acgcatggga cggcaatgat gatgctgaca atgcaggcac agatcagtgg acaaacgaga
     4141 acggaaacac cgactgggaa tatgataatg gcggcgatca gggctatacc gacaatggat
     4201 atgctgacaa cggctatgtg gacgatcaga cgaacacaga ttggtaaggg tcaggataac
     4261 taattgtata gtatacacaa atgaaactga aaaatcagtc gttttattga taattaataa
     4321 aaagtttata atggtttcat ttaagttctt tactttttga caaaagtatg ataatatatt
     4381 attgtatcta tttatgtgca tattcggcac ataggctttt ttgcatttta tttttgattt
     4441 gataaattga tatgtaaaaa tacggagaag tatgaaaatt cgcttttagg aggagttcta
     4501 ttatgggtta cgagtatgtt gaagagccag tcgtaggcgc aaagatcaag gtaatcggtg
     4561 tcggtggcgg cggtggaaac gcacttaact gtatcgcaca ggccggtatc gagggaaatg
     4621 ttgaatatat cgctgttaat actgatatcc aggcactgca taagtcagct gcacacacac
     4681 agatccagat aggtgagaag cttactcacg gtctgggtgc aggtgcaaag cctgagatcg
     4741 gcgaggcttc cgctcaggaa agcaaggacg agatcgagaa cgctatcaag gacgctgata
     4801 tggtattcat cacagcaggt atgggcggcg gaacaggtac tggtgctgca cctgtagttg
     4861 ctga
//
LOCUS       DAWEMR010000190         4732 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6690_length_4732_cov_10.130425, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000190 DAWEMR010000000
VERSION     DAWEMR010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6690_length_4732_cov_10.130425"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3714..3723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1294 a         1005 c          970 g         1453 t
ORIGIN      
        1 cgtgaagtat ctcacgaaaa tgcagaagct ttttatatcc cagcttacag cagtcaataa
       61 cgataacgtc aggagtatat tttacagcac ctatctcaag ctctatttca gtatcaccgc
      121 atatatggac ctttgtaccc ttgcgtttca gttcctcggt caacagttta tgctggccgt
      181 catatgactc ggaaataagg accgaataat cagtcagcat acacttcaac tcccttatga
      241 cgatttaaca taataactcc tatattatta tatcaaattt tttgactaca agcaacttta
      301 atggcacatt attaatcaaa ttgtttcaaa aacgtgacct taatgtaatt gtatataaca
      361 aaaatattca taaatgtcga tttaacgtat tgcgacagac gttagatact tttcttgatg
      421 tgttccaatg cacctttttc aagccgtgat acctgtgcct gagagatgcc aatatttttt
      481 gctacctcca cctgagtttg tcctttgaaa aagcgcagga agagtatatt acgttcacga
      541 ggctgaagct cgctcatggc ttgcttcaat gaaagctctt caagccagct tttatcgtca
      601 caggagtcgc tcacctgatc caatacataa agagcatcgc cgttgtcaga atacacaggc
      661 tcatagagtg agaccgggtc ggttatggct tcaagggcaa tgaccacctg actaggcttt
      721 tcggtgatag tgtctgctat ctgctcaaca gtaggctctt tctggttttc acatatgagc
      781 tgttctttcg cctgcattgc tttataagca aggtcacgga gagaacggct caccctcatg
      841 ggttgaaaat cacgaagaaa tcttctaagc tctcctgcta tcataggcac agcgtaggta
      901 gaaaacttca catcaagaga ggtatcgaaa ttatctatcg ccttgataag ccccacaaca
      961 cctacctgaa aaaggtcgtc aggggagtcg cttcggctca caaacttctg caaaacgctc
     1021 agcacgagcc tgagattgcc gtttataagc tcctcacggg ctttcttgtc acctgaatgg
     1081 gcttttttca aaagctccag cttttccgac tctttcagcg ttttaagctc attggtgttc
     1141 acgccgctta tctcaacttt attgtactgc atcgcacgct cctgttaaaa agcagcattt
     1201 tatgctgccg tgataatata attattacct ttcgatcagg aactatacag atatttttca
     1261 ggaaaatctg accataaaaa aatgcagagc cgtgtgactc tgcattagtt ctattctttt
     1321 ctgtcgctct tgcgctgata gtagaccgct agtatcgctg ttattatggc tgatatgcaa
     1381 aatgtcagca gaagcttgtt ggatattttt ctgtttatga aattcattgt agggttgagt
     1441 atatccaata tccagaatac aatgaacatt cccgcaagta ttatcgtgat atgtgggaga
     1501 attttgctta gtcttttcat ttttatcctc ctatatagat aatgtcgctg aggtcacgtc
     1561 caccgccgtc aggagcgttc acaatgctgt tgttgaagta catcatagcg gactttccgc
     1621 catcaagatt gaacgcagac atacagccct catcgctcat gatagccgca agctcactga
     1681 gagttgcgcc ctttgagtag ccttcgtttc tgccgtcaac tgtcacgaaa atatagtggc
     1741 ctgccgacac acagccgata gctgaacgtg gatttgcact gttgagatat tttgttgtgt
     1801 tgaacgtttc aagaacgttg ccacttccgt caagaagtgc aggaccaaag ttccaagcct
     1861 gccatgcacc ttttgctata acagcgtcgc tgtcatattc agacggggag taggtctgca
     1921 ttgtgccgtc tgtgaacagc acgcacacgt ctgcgtcgtt gacatcatca cggtatttta
     1981 taccgtttct tataacgata ctgcgttctg aattaccata gaaatcaccg tttatggcga
     2041 aaagagcgtt gttttccacc gccatactaa atactgagtc cttgatgttc ttaccgaatt
     2101 ctccgtctgc aaaggcggct tttatctcgc ttgcgcttgt gacataaaca tcagccacaa
     2161 aataggttat cttgttgctt ccctcaccga tagatttttt gtataatgtc acctgagcat
     2221 tgtcagttga agccgagcca agctcctcag aggttatggc accgtttgtt atactgctta
     2281 tagcgttttg gtctgaacga tagtcggcag tgtcctgatt gccccagttt ctgtggttcg
     2341 tctgctcagt acgcagtgtg gtcgtagcct tttctgcctg cggagctgtt gtggtaactg
     2401 caggctcgct atcgtcctgc gcagtgcttt ctgcctgcga gttgtcagcc gcatttggca
     2461 gggtgaagct accctctgcg gagctgtcaa ctgttgcgat cacttctccc tttactcctg
     2521 aactttgcgg cataacgtag tcaaaaagca tgaacacgcc tatcgctgct gctgtaagca
     2581 caacgtccgt tatgatcatt gcccagacag gcattggctt ttttgtctta ttcgtcaatt
     2641 tttctttccc tctttctgaa aacgaaactt ttttgtgccg cccagcttat aaagaaaagc
     2701 agcacttcta taagtatttt gctgatatat ttgttgtgta tgagatattt cacacatagc
     2761 tctagcatta ttgtgtttgc agctagtatt aatgcggcaa gcagaaagta ttttgcggct
     2821 gtctttgcaa tgccttcctt atttttgaag acgaattttt tgttcatgga aaagttgaaa
     2881 atggagctta tcactcttgc acatatgtta gaaaacgcta cgttcccgct tatcattgcg
     2941 aaaatgctgt agcaaatata gtccacgcag aagctaagaa gtgatgagca tgagaatttc
     3001 agtatatcct tgtatattct ccatgagtct ttgataggat taaaatgtga tgactcgttt
     3061 ttgccaaggt aaactgtttc gataggcact tcatagaatc ttatgccttc tcttgcacat
     3121 tcaagcagca tattcatctc aaactcatat ctgtcaccgt ttaccgacag cataaaaggt
     3181 atgagctttg aggaaaaacc acgcaagccc gtttgcgtgt cgcttacctt tactcctgcg
     3241 gcaaatgaga aaacgaattt tgtgatgttg ttgccgaatt tgcttcgcag tggtatcttt
     3301 cctgaaaatc tgcgacaacc cataataagc ttgtcagggg agcttttcag agcttccgac
     3361 actcttatta tatcctccac cttgtgctgt ccgtctgcgt ctgcaatgac gatgcccaca
     3421 gtgtcggctt catgttcttt tatgtattca agtgcggttt ttatgccacg ccccttgcct
     3481 ttgtttacct catgggtgag aactatggct ttttttgcgg cttcggcaaa aatcggcagc
     3541 ttatccttgc cgctgccgtc attaatgaca attatatgaa aatcggtcaa tgaagatata
     3601 tcgtcaataa cagccagcat tttttcatca ggctcataag cgggtatcac cgcataaaat
     3661 ctatctatcg ccaagatatc ttctcctttc atttatcgta tggtctaagt atannnnnnn
     3721 nnnatcacat atgctttaaa attacaataa aacttctgaa gaatttatac atagtttatc
     3781 tgtcgaaatt ttgcgaaaat gaaattgtgt cagtattatt gacaaaataa taaaaatgtc
     3841 gtataataga agaaacaata aaaaatgtac aaattaattc agcaaacgga ggatttgatt
     3901 tcatggaata tagagttctt gaaacacgtt cagccgatat tctcactaag tattacaata
     3961 acgagataga cagctttctt aatgcctgtc atgaagacat tctgtggata ggccccgctg
     4021 agaagcatgt gatacgcacc agaaaggcgc ttgttgatgc tttttcgcaa gaggaacaca
     4081 agcttgaatt tgtgctcagc gacctaaagg tgttttccat aggtcttacg gaatgttcct
     4141 gcgaggtcat aatgtttttc attttggata cgatttggcc tgacagcagt gttgggagag
     4201 cagatcaaag gatgcagttt acatgggtga gtgagaacgg cgtagaaaaa ataaggcttt
     4261 gtcacatttc caatgccatt aaatatgatg aacgtgacag gatatatcct gtacattatg
     4321 aagaaatata caagaaaaat gtctgttcag gtgaaaggcg tacaaagaga gttatagtaa
     4381 atggcgtaga caggtcgctt gtttttctgg ataggtcatg gatagcttat gtggaaagca
     4441 gtggcagtca ctgcgtcata tataccattg acggctcatt tgaatcagtg cagagcctta
     4501 aggtgtttga aagacgatat agggatatgt ttgtgcgctg tcatgaaaat tatcttgtaa
     4561 atcctgaata tgtaagccgc ataagccgtt acagaataga gcttacatat ggcaaggtga
     4621 tccctatttc tgagaagaat tacactgctg tgaaaacaaa attatcccat ataacataaa
     4681 aaggggctgt aaaaaaagtc cccaagaaaa aaggctgcga tcttgccaaa aa
//
LOCUS       DAWEMR010000191         4721 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6709_length_4721_cov_8.067724, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000191 DAWEMR010000000
VERSION     DAWEMR010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6709_length_4721_cov_8.067724"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1610 a          867 c          909 g         1335 t
ORIGIN      
        1 gatcatatgt atttcctcct tataatgtag tataaatcac atcaattata ttcacaatat
       61 aaataatcga caaatttcgc cgatttgtta taaataatat aaacttgtga cttctaaata
      121 tatttatact ataataaaaa tgttggaatc ggaaaggtag gtggatcgta tattatgaaa
      181 aagcgagtta aaagctcaaa tgtttttggc gaaaaacaaa agctcattat cgccaaaatc
      241 ttgatcaatc tgcgagagct aaactttcta acacaaacag atatagcaaa acatcttcat
      301 ctttctccta gcacgatttc tcactatgaa aaaggattaa ctgtcccacc tacagaagtt
      361 gtgtatcgac ttgccgactt ttataacgtt actacagatt atattctcgg caaaagtgca
      421 agcaggacaa atcttaatga cacttatgca ataaagctaa caaataaaat gactataggc
      481 gacgctgtag aaataatggc aaagatggac aacgcagaac gtgagcattt agcgtatttc
      541 attgaggtta taggaaagtc aaaaaaataa ttcaatttag aactctgaat ttgtatcagg
      601 gttctttttt attttaccat aataaaacaa caaggtcaag catattatgt tagttttctg
      661 atctttgcat ttcgtgctaa acgtcttcac cttatgccaa ttttgttgac atcgataatg
      721 taaaacgtat aattaaataa gactcatgca tcatatgaaa tttaataagg agaattttta
      781 tgactataaa tgcttggaga gcttcaaatg taaaaaattt tgatcaatat actgaattgt
      841 acgattttgc aggtgtttct gtttttaaaa tcgtagttca gtaaagaaca caaaacatga
      901 ctgacaaact cagattcggt cagttcatat ccgagaaacg aatacaacac tctatgacca
      961 agcaagaact cgcaagacag attgggatat ctacagcata tctcagtcaa cttgaaagcg
     1021 gagcaagaag caatccaact attgaagtgc ttgaccgaat gattaataaa ttgcacttgg
     1081 acgaagcgga aacacataaa atatatgatc tctatgcagc atcaaatgat acaattagtc
     1141 cggacattat tacatatcta aagagcaata atattgtaac aaaagcgatt cgtaaagcac
     1201 aggaaaatag tgcaacagaa aaagattggc ttgatttcat agaccaactt caaaaatgaa
     1261 caaataattt tgcggtttat gccgcaaatt tatttagcat cagtttagct taccagctaa
     1321 acgtcggagg attgatagat tgacaacaga taatatacat catatacgct gcccaatatg
     1381 tggtggcaga ctttttgatg taataacagg aaaaggcgag aatagttatt tcggtacaaa
     1441 ctttgcagtt atgatcaagt gttggaaatg ccgagaaaca ataacgttaa aaccattaaa
     1501 taatattaat tacttagtac aagcatcaca cgtttgttta aaatcggagt agaagcctga
     1561 caagtagctt ttaaagctag tgtcggtttc tattttttta tttttgttaa ctccattttt
     1621 gctttaagag cagaaatgga gtttttttat gcccattttt aaatactggc agcaatacat
     1681 agccaaatat ccataagcct tcgttttgat tcagaacaag aaaatcaaaa cggaggaata
     1741 aaaaatgagc aaaaacatag taagtgttta tgacactata accaaaagat acgttgaaat
     1801 tgaagtaagc gaaaagatcc gcacatatta caatagaacc caatggaata ttgatgacaa
     1861 caataaatcg ttctataagc acgagatcca gttctctgcg ttgaaaggaa acattaacgg
     1921 agaacttgaa aactttcagg agttccgaac agaaaatgac gacgtcgaaa gaagagtgat
     1981 acaaaatatt aaatgcgaaa agctgtatag ctgtctgaat gaactttcag aggatgaacg
     2041 aaggttgatc tttgcaatat attttgaaaa taaaacggaa aacgaggtcg cttgttttct
     2101 acataccact cagcaaaata ttcacaaaaa gaaacgtagg attttgtgca agcttaacaa
     2161 acttttgaaa ttgcaataaa aaacggttgt taaataccag acttctaccc ctatacaagt
     2221 gtaaggagaa taaaagctcc aagtaacttg acaatagaat atcaactctg taagtatgtt
     2281 aatcacacgg ccggtatgaa atctcggcag ccaaagaaac catacgccaa gactccgaaa
     2341 ggatgagcga taaatatgac gtttcaaaat tcgatgcagc aaaggcaaaa ctggatgaaa
     2401 cgcagtcggc gataaccaag cggcaggctc agagacagat gatggagaac ttcatgaagg
     2461 tgctgcggtc gctgccggag caggtcgatt actttgagga aggcacctgg tacgcaatgt
     2521 gcgacttcat cacggtctac ggcaaagatg acatccgggt taccttccat aacggattgg
     2581 aaatccgagt ataaagatta ctccccacaa cggctcactt tgaaccgatg tggggagctt
     2641 ttctgtctta ctttgtaata gccgaaaaat cgggctcacc ttttgttatg atgctgaaaa
     2701 tcaaatctgc cgtttcttga cttaccgcat ctgcatcgct tttgtctcca taacactcca
     2761 catagacatt cgtatttcca atagagaacg aagcgaagaa tattgcggat tgctcacctc
     2821 tgctgttggg actggtaaca aagtatcccg tagtgacagg aattccgttt accgatgagc
     2881 tgctttcgtt tccttccaga accacatcgc ttattggaaa attatcctgg gcaaatatga
     2941 ttgtcgcatc accgagtttt ccttcgactc ggagcagctc cccgttttcc tctcggaaag
     3001 ttccgatggc tgatcttcgt tcagtggttt caggaagtat ggcgtccaat tctgcatctg
     3061 tcagtttacg cgatgaaacg ggatagccga catgaatact tgccacgttg gtacttccga
     3121 tgtgatagac aatggagtca ccgtttgcaa gattaacagt ataaccttta gaaccaaacc
     3181 atcccatatt gctgccaaca attcctgtta ctgcgactat ggctaccagg caagcggcaa
     3241 ctggaatcag gaactttttg aaattctttc tctgtgacat atagttcacc ttatcctttc
     3301 tgtcatgaac agaacggttc ttctctaaaa tcgcagaaag catacgatca tttgcagaat
     3361 cgctcggttc gattttgtcc caagaagtaa tgattttatt gttcttcatc gaaatcatca
     3421 cctaaccttt ctttgaggat ggcacgagcc tcgtgcaaat aattacggat tgtcgattgc
     3481 ggctttttca ggatgcctgc gatttccacg ctggtatatc cttcataata atacagatag
     3541 accacggtct tatacttttc ggggagttcc ataaccactt ggaaaatatc atctgtttcg
     3601 atattttctg agctggggag attgtagaaa tcctcgatgt tctcgtgtct gcgccaccaa
     3661 tgccgcagga catttttgca aatgttcgtg gcagttctga taagccatgc cttttcgtgt
     3721 tcttcgcttt caaaagcggg accgctcttg attatctgga agaatgtatc ctgaactgca
     3781 tcctcggtat cagcaggatt tttcatgtaa gcaaagcaga cgcgataaac agttttactg
     3841 tggcgtttgt agatctccgc aatctctttg tccgtacgca acaaagattt tgacatcttg
     3901 ccttgccctc ctttcactac taatacaatt gagaacgcca aagtgtcgga taaaaaagaa
     3961 aaaatatcag aaaccattat aacgccagtt tctgatagca atatgataaa attgaaaatt
     4021 ccgagcctta tctacggaaa ggttacaaaa tttgaacccg tccgagaaat ttgaacccgt
     4081 ttttctgtaa aaataaaatt gtatcagaga cggcttttag atagatgagg gtatcagcgg
     4141 aacgcagaca aagaaaagaa cagaatttaa tcgtatgata agaatgtgca aaaagaagaa
     4201 aatagacctc ataattacaa agtctataag ccgtttttca agaaacacgg ttgattgtct
     4261 tcagtacata aggcagctga aggaattggg tattggcgtt attttcgaaa aagagaacat
     4321 aaacacgctg acaatgacaa gcgagtttat gatagctttg tatggaagtt ttgcacaggc
     4381 cgaaagtgaa tctatcagta agaacatact ctggggaaaa gaaaaagcat atagagaagg
     4441 gaaagtaaat tttcagtaca aacatcttct gggctacaaa aagggagcag acggcaaacc
     4501 agagatcgtt cctgatgagg cggaaacagt aaagctgata tataaactat ttcttgacgg
     4561 ttatagtatg aaaggtataa aaaatgttct tgaaagcaaa aagcttctta cctcaatggg
     4621 taataaaatc tggaacgaat cttttatccg cagtattttg aaaaatgaaa aatatgtagg
     4681 agacgctttg ctgcaaaaga catatacgct tgactgtatt a
//
LOCUS       DAWEMR010000192         4715 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6716_length_4715_cov_10.588627, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000192 DAWEMR010000000
VERSION     DAWEMR010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6716_length_4715_cov_10.588627"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1267 a         1009 c          803 g         1636 t
ORIGIN      
        1 ttatcgctcg ctttttcaag gtcatggcgg acatcacatt tggctgccga gatttcatac
       61 cgaccgtgtg attaacgtac ttgcagagtt gatattcggt tgtcaagatt ctgtcgagaa
      121 aattctccga attagtttca tcgattttcc ccttcactac ttactggcaa cgaaattgag
      181 atttgtaagg gatttatttt tcgttttcaa ataatttcag cactttcacc aaaatacgat
      241 gtactttttc gtgcattgtt tgtctggtaa cactatattt catggcaaga tcattctgac
      301 ttttattcat ccaaaaaagc tgataaataa tttcacgttc gtatggcttg agttttttca
      361 tgaccttttt gagctttacc aagtcatgtt ccaagcctaa ctgctctaag atgcgctcct
      421 caagcggaac aactgtactg tcattgagaa tcttctgctc gtaatttccg ccgtatgtat
      481 actgttccag cgaaaccgga ataaccttct tccagttcct atccttttgc gtatattctc
      541 cgtgatcctt cttggaaaag tgatccgaat actggctttc ctcgcattcc agaacgctgt
      601 cgccgaagct gataaaccaa cggttttctt cttcctttgc attgagatac tcttcctcag
      661 taaattcgga atacttctca cttccgccgt ttctgctaaa ttcaaaatat tttgttttca
      721 tcgtaattcc tccgttttga tttttcttgt ttgaatcaaa acgaaggctt aagggtattt
      781 tgcaatttgg cattgccatg ccttaaaaat aggcataaaa aaaatcccat tccgctttta
      841 aagcagaata ggagttcaaa aaatgaggca gaaatcctga catagcttta aaagctacct
      901 gtcaggcttc tacctcattc gtaatttctt actaagactg catacgcata tacagcccaa
      961 atcccatatt cacagatggg tggatgatgc tctgtacgag taatttgata tacttatttt
     1021 atttatattt ctcactttca attctctgcg gtcagaccta tcagtttacc gcatttgtga
     1081 cacttcgtga gataatcacc ttgttcggct gttgtggcta cgatcctgtt tttgcaccat
     1141 gtgttttgct tatccgcaag ttttcctccg cacaacggac atctcagcgg cggcatggat
     1201 ggtctttttc cgacttgacg gatctgtgaa tcaactaaca ttcttctgtc ctttctgttc
     1261 gttttttaga gaacattatg agtaaaaata taaccgaaat tcggttatat catattgtgt
     1321 gaagcaaggc agatcttcat tgcttgcctg gacacctgaa acagctctgc catttttaaa
     1381 attttagcgt taacgtttga tccgtgcatc gagaaaaacg ccttttttat ctgcattttc
     1441 ggcataagta tcgaagcagc aagagcgtct gcctgccatt cggttttaga attgctttct
     1501 gtcgttgtct tggcggcgga ttcctgcgaa ttatcaaaaa tcttatggtg cagtatccaa
     1561 tgtgaaagct catgggcaag agtgtacctg tatctgccca tattttcttc ttcaagcaaa
     1621 gcttcatcga tgagtatcgt tccgggctga acctcgataa tagtgtactg ctcattttct
     1681 ttgtcataca cagggacaaa gcagttttca aatacagtct gaccgaggac gtttcccgat
     1741 tttctcagtt ttacatattc tatcgtcagt ttgtattcaa cctcgatgat ttcctctatt
     1801 ggtatggatc ggggagcacc gtaaagatac atgccgtttc ggtaattttt caaaagtccg
     1861 tttgcaatgc agtcaagagc gctttgtctg tagaacattt cgttcatggt tttccctccc
     1921 tgctttttct aagcttatca ataaaatcct gccattcttc gtccgtagcg tccagatcct
     1981 ttgcagttct gagagcggaa acgatcacac ggttgtcttt cagaaaagca ttcagatctt
     2041 tcggtatcgg attttttgcc gtctgctcca gagcggcaag gtcgtaaaac ctttcggatt
     2101 cctctttatt cagcgccagc ttactcacca ttttttgcat gacttccata ctcggagccg
     2161 cttttctgcc tttttccagg tcgcacagat atacgggaga tatgtcaacc agcttcgcaa
     2221 agcccctcag cgttatttcc ttatcaagac gtttttgttt caggtattcg ccaaaagtca
     2281 taaatcttca atcctttcaa tccttgttct cttttctgcg aacgtcttgt atctattata
     2341 tcgaacgtaa attcgattgt caatagcaaa aaatgaggtg cacaattatg aatgcacctc
     2401 aatgatagtt tctcccattt gcacaaactg atctattgca ttttgtggag attgtataca
     2461 aatttcgccc tgatactgaa acagccacgc ataaaatgta ggactcagct ctacttttac
     2521 ctttacgcag aaaaattgac gatcggttcc gattgtatca atattgtctc caaacttcac
     2581 tataagggct ttcatgtgct tatttttaca tttcagtatc acgtctgaag actcaccgca
     2641 gaacatttta aaggtatgtg caataaagtc cttcagcaca aaatctttcg gacacggttt
     2701 ggaaggatgg tctgtcagag atatattcgc taatctgtct atcctgaaaa ccgttatatc
     2761 tctgtgtttt tcacaatacc ccacaaggta atacctgtcg tcgttccaca cggcggcata
     2821 ggggctgacc gtatatgtat aaccgttatg cttgagaatt ttttctcttt tttcattata
     2881 ttcgtaatac tgaaaacgga tcttacgtcg ctgcgtgatc gcagtgttga tgatatcggc
     2941 ataatatatg cttttactgt ccgccttgtt ttcgctcggc tgctgaatga gatttcttat
     3001 ttcttccttt tgatagtcgc tcagaggaga aaacagcttg tctatcagct ggaaggattt
     3061 ttcttccgaa ataaactgag ctgcgtttac tgcgtcgcac agcattttta tctccgaaag
     3121 ctccatgccc ctgctgccca taaaatattt attctgcgta cttttatcgc agataatatc
     3181 gtagcctgcg tttctgagct tttctatatc agaggggatc gtgtttcgat gagcatatac
     3241 gccctgctcc gacagttttt caataagctg atttgtagtc agcttatgat tttcatctgt
     3301 ttcttttatc agtatttgca gtaatgccag aagcttattc atacgtcacc ttgagatttt
     3361 ataattttga ttcttatatg tgatactctt tatttttccc ttgcaatcgt attcaatttt
     3421 ttggtttatc gccaacaagg agattgccag cattgataaa acccctgcct tgtacggatg
     3481 attatccgtt tttacaattt tctcaccaca tcactgccca taaacaagag gaacgcctga
     3541 tgctaatgat ggatcaatat tatcaagaat agaatcaaat actgtattct tgatatattc
     3601 ttttaatgat tccttgattt taacatcgtt agtaccaaga taaaatctga taattgcaag
     3661 ttttacaata tccaaaaaat cattataaga cattctataa gtatcaccct caaaagtttc
     3721 aaattgataa tactctttat tatcatttga tttttcaaaa aaacaccaac aaagctcggc
     3781 tgtccagttt tctttcttat taacacattc aataaacttt ttaaagtgac acgcagctaa
     3841 cgtattaata agtaattcaa cacattcatt tgcctcatta tttacaagta aattcataaa
     3901 aagctccttc tatttaaacc gatggataca tggtaataag cttattatct ctaatataaa
     3961 aattccatgc ttttccagaa ttatcaagtg cagtaacagg tcctccactt acagattcag
     4021 gaacattagc atacgcgtcc aagccgcgat caataaaatc atcagtgctg attacatttg
     4081 aatcatatat cgtcttagtg gcttttcccg tttttgtatt tgctgtatat tcaacataag
     4141 ttacaccgtc tataccactt acattttgga tattatctgt tagatcacca ccagcaatat
     4201 caagttcact taaaaagtga ctttcttcat ggcagccatt tatcgtaccg tttggctttg
     4261 ctccagtatt gtcagctgta ccttcaaggt gttccttaag ggattgctta ttttcaggtg
     4321 tgcaattctt gttatgcacc caaacagcac aatcaccaac aaaataagta tgataatctt
     4381 caacctgaaa attgtaaaca gtagcaggct cgtgaagttc ttcaatccga tgtttctcaa
     4441 caaacaaatc ttcgccattt acgctgataa gtttatcacc aacgagaaga tttcctgcat
     4501 tgataaagcc tctgccttgt acataaaacg ggtgattttc ggttgtgata atttatttac
     4561 cgttaattgt cagatgtaca atcttgtata cttgtcgggc atatagtgca agattcgctg
     4621 attattaaat gtaccaatat tcaaatctta attccacttt atctcttcaa gttgagcaat
     4681 aacattatta acataatctt tttcgaggat cattt
//
LOCUS       DAWEMR010000193         4633 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6842_length_4633_cov_9.376802, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000193 DAWEMR010000000
VERSION     DAWEMR010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6842_length_4633_cov_9.376802"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3985..3994
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1292 a         1073 c          858 g         1400 t
ORIGIN      
        1 aaggaagccc tttctattgt aacattcagc ataaggaaca tcacagagca gaaaattatt
       61 acagcaaccg caataagcat tactatcatt gacatcataa ggtccgtgaa aactgccgct
      121 gaacgctcaa tatccgattt tgttgttgtg ctgtaaagtc ttccctcgtc aatgtcaagt
      181 ttcttgtctg aaagcaaaca gttgtagtaa gtgtcgctct caccgaaaag ctcacgcata
      241 ctgtcaatat ccatgaacac cataagtccg ccacgctcgt cgcagatatc ctccactgtg
      301 aatgcgtaat ccattgaggt cgcatcgtct gtcaggataa acttatcgcc ctttgccact
      361 ccgtaacgct ccacaactga ctgactcact gttataaagc tcttgccctt atgtgttttc
      421 acatcataat acttgttgtc gctgtccata cccataacgg tgacatcaag gttatagcca
      481 agctgttcct ttgaaagcgt ttttacaaag caagcctcgc caccctctgg aacgctttcc
      541 tctggatatt tcagagtgta catatactcg tattttgtgt catttatagt gtcttttctc
      601 acgttctcac agagatagta gcaatcaacg ccaagcataa gaatgaacag tgaaaacagc
      661 atactgagaa gcactgttat gcctgtgcgc atttcacgaa gcatttggcg tatctgaaat
      721 ctgcggataa aattcctgct ctttatcttc actctgctgt agtgacctgt tttctgctcg
      781 tttctgataa gtgaaagagc cgtctgagaa agccgcttgt ttatcacaag ggtattaact
      841 ataaacgaaa caacaggagg cattacaatg ctgtaaatga taagatacaa aggataaaca
      901 ggggtaaaat cagggagcga tgaatatgcg tagctgtcga gaagctgata atcgattccc
      961 acaggtgaga agcctatgac cgcaccgatt attccgccaa caaatgcaac tatagtcggc
     1021 aatgttacgt agtgtcgtat aagaactttt ttctttgcgc caagagcata aagcgcacct
     1081 ataacactgc tttcacgctg aatctgatga atgacgaaaa ctgagataac gtaagtaaga
     1141 agtgccataa gtataacacc cacaccaagt cctgcatatt tgttcatgac aacgtcacct
     1201 gccgcaccag ctatacgaac gttgtcgccc tttttcacaa acattgtcag gttgtcaagg
     1261 tcaatggtga aaacctcgtc aagaagctcg tcagcctgct ttttgaagct atcaatacca
     1321 tttgcaaggc tttctgcacc gctgtatgca gatcttacac catctgacag atcttttgag
     1381 ccgtcatatg cggctttaac tcctgccgtg atagcgttcg caccctctgg gagtgccttt
     1441 gcaccctcgt aaagtccgcc cattgcgtca tagagagcgt ctgctccctc agaaaggtct
     1501 ttcacaccgt ctttaagggt ctgtgagccg tcataaagct tgtcgatacc atttgaaata
     1561 tcgtcacgct gcttcaagac gtccttgata gtttcaagat aatacttgtc tgtgactttt
     1621 ttataatcaa agtcaaaatc ctcgatcatt tctttaagct tgtcatctgt tgccttgccg
     1681 ttaagtctgt aagcatagca aagatcctct gctttctgtt cactgtcatt tttgaaatag
     1741 tcatactgat cgtcgcttac gaaaagaagt ccgaagccct tgcttgacac agccgtgtca
     1801 gagaaatttt caaaaggtgt gtcatagtca ggtgtagttc ctattccaac tatctcaaac
     1861 tcaacaccgc ctgctgtaag cttatcgccc actgacagag aatgttcctc gctgtaacgc
     1921 ttttctacca ccgcttcgcc ctttttctca gcaagtctgc cactgtcaag ctctataagg
     1981 tcaatgtcat ttcttttacg gaaaacacga agctttgagc cgtccttcgc ggtaacgtca
     2041 atgctgaaat gctgttcaag agtaattcct ttgtctgtta tttccttttc ctgctcgtct
     2101 gtaagaggaa taaaaacgcc gaactgtccg tcctcgacct tgttttgctt accatgttcg
     2161 gcagttccgt cgatgatagt gtctgcactt gcaaccacgg aaactattat gtacattccc
     2221 attacgataa gcaatacaag cgcaagataa cgtgcgaaat ttgttttaag ctcacgtctg
     2281 agtctttttc tgagtaccct gttcattaca agtcctccaa ctctgcggca ggtatgcgtg
     2341 tttcgttaag ataatccttt ttgataagtc cgtccttaac gattattacc ttatgcacca
     2401 tattttttat agcgttgttg tgggtaacga taagcattgt tgtgccatat ttttcgttta
     2461 tcttctccaa cagaatgaga atatcccttg aagttttcga gtcaagagcg cctgtaggct
     2521 cgtcacacaa aagaagcttt ggatttttta taagtgccct tgctatagca catctctgct
     2581 gctgaccgcc tgaaagctgt gacggaaact tgtttctgtg ttcggaaagt cccaacgttg
     2641 tgagaagttc ttccatgtcc agtggcgaat ctgaaagata ttcacaaacc tgaatatttt
     2701 ccacaactgt cagatttgga acgaggttat aaaactgaaa gataaaacct agattttcct
     2761 ttctgtagtc ggcaagctta tcaggtttaa gaccgaaaat ctccttgccg tcgaccttaa
     2821 cagagccact atccatagtg tcaaggccgc ctatgcagtt aaggagcgtg gatttaccag
     2881 agccgctcgt accctgaata acgcacatct gacctttttc cagacttgtg gtgataccct
     2941 ttagcacctg aatatagctg gcgtcactgc cgtagctttt cttaacatct ctgatctcca
     3001 aatacatatc atttcctcca tttataagtt agacttagct aactttactt tatataaaaa
     3061 tgtgcatatc tgcacatttc atcgacattt gagatacaaa attcacacat aacgttgtta
     3121 tgtaggtttc taaaagttac cgcccgcagg cggcaatttt ctaatcctcg actatcattg
     3181 taaaccatat ttttattaga aactccatta ctctgcgcat atggagcttt gccttttcca
     3241 catctctctc gtgagttagc aaatgagtgt aggcgtcaac acttatatgg gttatccaat
     3301 gaagcatata ctcatcaacg tggcagttag gcattcttgc ggcagctttt tccgctgata
     3361 ttctaaagcc ctgttctgtc atatctacga tcatatccac agtattttca tactcagacc
     3421 cttgcgcacc cgtgagcagc agcataaaaa cgtcatagtg gagatacata taatcaacaa
     3481 gcttgtcaag gtcggtgtga gtatcaatgt gatactcagc agacagcacc gcagggtcat
     3541 cgctatccat atcctgagcg tcctgcatat aatgctccgt aagaagcctg caaagctctt
     3601 tcagcggctc atcgacaata gccttgaaca agtcattttt atttttaaag aagaaataca
     3661 gcgcaccggt cgtaacgcct gcgtcagcgc atatttttct cagagaagcc ttcatataac
     3721 cattttcaga gaactcggcc attgcacttt ccaacagctt ttcccttgtt tcaagttcgt
     3781 cacccattac tgcaccccgc tttccacttc acataacact gttatcttag catataacgc
     3841 tcgatatgtc aatacattta ctatattttc agcatattat atgatattga aacataacag
     3901 tgttatgtga ttggtgacta ttacaaaata aaatgggaag cgtcatacct gtctggtcag
     3961 attttacgat ttttgacggg ttaannnnnn nnnntatata agcgatagct ataaaattgc
     4021 tatggtttac aactgcgaca gcgctcctat gctagtcctt gaaaacaagt actcatatag
     4081 ctgggatatt tgcattagtg caagtgaggt gtcgtcagga gttgctttct actcatacga
     4141 tgacattgca caagctttca gcaactacgg agtaagcatt tatgatatgg gacagcttca
     4201 catatttagc tctaattata ccgagatcta cggagtatat gcggctccgg tctgatatta
     4261 caacatagta actattcaag tcaaataata atcggaccga gcaaaatgtt ccaccggaac
     4321 atcttactcg gtctttttcg tagtattgat aattcaacat atttacaact atcctgtatt
     4381 tttcacgcaa tcaccaatct ggtcattttc tctatccact tatcaatgaa acttataaac
     4441 ccctcaaagc ttcatagctc tccatttgtt tgaaataacg ctgatattcc agtatgtgat
     4501 aaagcagata aacaacatac tgattgccga tacgacaact gcttcaataa taagaaacgg
     4561 tgtcgaatag gttgatatga atcttgacgt aattgtcaaa atgatatttt gacaaaacct
     4621 aacaaatgtc ccc
//
LOCUS       DAWEMR010000194         4576 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6929_length_4576_cov_9.042026, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000194 DAWEMR010000000
VERSION     DAWEMR010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6929_length_4576_cov_9.042026"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1262 a         1030 c         1043 g         1241 t
ORIGIN      
        1 gacgcctttt ttatgtcgct ttttatatcc ataatagaca aacaaacagg cttcaaacag
       61 ttgcaaatgc cgaaaatgga gcgtttctat tgcttccagg actttgttat ggtacaatca
      121 tatgtgcaaa gtaaaacgtt tacataaaac agaaagcagg aaatatatga acgcaagaaa
      181 aatattatgc cttatggccg cagtgcttat gctgtcaggc tgttcaaagg gtgtggacgg
      241 ttcacaggct tcatcaaaag cggctgaaag ctccggcaaa gcggacagca gtgtttcaga
      301 ctatgataat accaccaccg agcctgaaac agacaagcct gattttgacc tcaatgacag
      361 cgtgataggt ttttcacagg aaagcggctt ttatgacagc ggtttcagcc ttgagcttac
      421 cgcaaacgag ggtgagaaga tatactacac cactgacggc acagacccca ggacctctgc
      481 cacgagagaa gaatacactg cccctatcga gataaaagac agaagcaatg accctaatgt
      541 ggtttcagcc gtgccgacag aaatgatatc gggaaatttc aatgagttca gctttggcga
      601 aggggatttc tggtgcaaca aaaaagcacc ttctgacaat gcggtggaca aatgcactgt
      661 tataagagcg tcaagcgaaa aaagcgacgg aagcgtttca caaagcttct caggcagctt
      721 ttacatagga agcgcagagg agcatataaa agggcttaaa gaaagctgtg aagcggccgg
      781 gcatgacctt gcggtaatga acatcacaat ggactacgct gaccttttcg acagcaagat
      841 cggcatatac gtcaagggag atattttcaa caaggcactt gaagactata aatccagcgg
      901 agaaagcata gaaaacgaaa ctgcaaggca gcttgacgca aactacaaac agcgtggcaa
      961 ggaatgggaa cgtgattgtc acatcgagct taacgagatt tcagccgagg gaaatgtgac
     1021 gaacgctttg tcacaggatt gcggcataag gatacaagga aactattcac gctccgacct
     1081 gcaaaaggga ttcaggcttt acgccagaaa gaaatacggt gaaaagaagt ttaattatga
     1141 ggtctttgag gggctgaaaa acgcatctga tgaaactatc gacagcttta agacgcttac
     1201 tcttcgtgca ggtggtaatt gtgcttttac agcaaaattc aatgacacct attggcagag
     1261 tcttatgaca gagcttgacg gctcaacaaa ggcttcacgt ccatgcgttg tttatctgaa
     1321 tggcgaatac tggggacttt atgtgctgga agaggactat tccgacaatt attttgaaag
     1381 ccactacggt gtgaacaaag acgatgtggt ggtctacaaa ggcgacgcag aaacctatca
     1441 gagcggctac aagcttgacg agggcaaact tccggaggga gaaacagacg agggatattt
     1501 cttcaaggag cttacagatt tcttcgcttc tcacaaagac ttatccgcac aggcagatta
     1561 tgaggagttc tcaaagcttg ttgacaccga cagtgtgaga gattatttcc ttgcagaggt
     1621 ttggataaac aacaagtggg attggccggg caagaactgg tcaatgtgga aggtgcagaa
     1681 cacagacagt tccaacgaat atgcagacgg aaaatggcgt ttcatgttct atgacgttga
     1741 gttcggcgga gtgagcggcg agggcgacgc atggacgaac accatgaaag aggataacta
     1801 caagccaaag ggactgcttg acacggacac gaaaaatcct gcggttctga gttttgctta
     1861 tcttatgtca aatgaagatt tcaggaacga tttcaatgac aggcttttaa agatgtctga
     1921 gggtacgttt gaaaaagaaa aggcacttga taggctcgca gaatttgaga gcatatattc
     1981 ccctctttat gagcagttct ttgcaagata cccagaaaca ggctcggctg aagaagcgct
     2041 tcatggagga tatgcttcat cagactgcat aagagcgttc attaacaagc gtgataagtc
     2101 tatccaaagt attgtggact ggacaaattc gcagttctga tatttcacaa accaaaacgg
     2161 cagaacagca tatgctgaac tgccgttttt atatgctctg atctatgccg caggagcgta
     2221 tgtgaatgtt atttcaaggg tgtttatctt tcctgcatag tcaagtatta ttggagaagc
     2281 ctttgaaagg tcaccgcctg ctatccttgc gtcctcaggc ggtgtggata cccactggtt
     2341 cacaagattc actcttgtgc aaagctggtc gtcagaggtc gtatagccct ccgctagggt
     2401 ttcaacagct tcgccgttta tctttatctc gtcaatagtt gcgatatagt tcggataaag
     2461 cttctcaccg tttgcgatac ccaaagccga gaatgcaatg ccgttggcgt tgtttgatga
     2521 catatcaagg cttactgtgt aagttccctc accgtctata actgcgtttt ctgcgataac
     2581 tccgtcgctc tttgaaaccg gatcatactc gtcaccaacg ctgtagcata cggaaaaatc
     2641 tgcactctga tacattatcc atgccaccgc tgtgttctcg tcagcctgcg gtgcggtatc
     2701 atctgttagc ttttcaggtg cagccgcaag agtttcatca agcttcttta cggcagtggc
     2761 tctttcctcc tccactgtca tcgaggactg gttatcacgg cgtctttcat caaatatctt
     2821 tgccacagtt tcatcacgca ttttaagctc tgaacgtgag aagaaatcac tgcaatccca
     2881 aaggaatggt gcaaagccgt aggcgtcaca gttgtcaagg aggttgtcaa taaacttgtc
     2941 agtgcctttc tttaggtcac cgccgtttgt aagcactgca tactcgccta ttattatgcc
     3001 atagccctgc tcggaatact ttgtcatgtt cttgaaaagt ctgttctgct cctcatagtc
     3061 tgtctttgtt ccccaatctg aacgaccctt tgttccgcaa taatcccatg gggtataata
     3121 atgaacagaa agcaggagct tatcctttgc ggtatcagtt ggcatctgga acttgtctga
     3181 gcaggtcatt gtgatatcgg tattgtagcc tgcgataaga agaaaacgct gttcgttgtt
     3241 tccgcctgtt gcacggacag tgtcaacaaa ggtctgattt atcttgttgg ccatttcata
     3301 gcactcggct tttgaaagcg agcctgagtt tttgcacacg tctgtgtcat ttagtctgtc
     3361 accaagctct tcgtttgcac cctcaaatat aagtctgtcg gagtattttt tatatctgtt
     3421 cgctatctgc gtccacatgg acttatacat ttcaaacgcc ttgtcaacgt cgctctgctc
     3481 ggaagaaccg aacattcccc accagcttcc gtcccagtgg tcgtttatga taacatacat
     3541 atcattatcc aaagcgtagt ttattatctc ctccactctg tcgagatagg cttcatttat
     3601 ggtgtaatcg cctttttcat actccattgc gtttgtccat gccacaggca cacggagaga
     3661 gtcaaaaccg cttgctttca tgccctttat catttcttcc gttgtgacag gctgacccca
     3721 aaaagtttca aagctttcag ggtcggtgtc tgcggaatag cttttatgac cgtaggcttc
     3781 catggtgttt ccaaggtttg tgccgttgcc gagaagtctt gcaagctcgg ttgcggtaag
     3841 gtctttgttt atgcttccgt catcagaggt ctttgcaccc tcctctgcct tagatgaaga
     3901 gctgtcactt ccctttccac agcctgcaag cagtgcagta cagcccatga ccgctgcagt
     3961 aagaagcggg ataagcctaa acttttccat gtcattccct cctatttcat aaatacaaat
     4021 tcttcaagtt caaaaagcgg cttgtttttg aaataatctg ctatccaatc ggcaagttca
     4081 tctgaaaaag ctgtaggcac aaaatagata gaatgtctgc cagtaaggga cttgattact
     4141 cctgtgtaaa ctccgtcacc tgtgccgata tcaacatagc ctacaggctc gctgttctca
     4201 tagtcatcag catagaccgc cacctttgca acagttccca tgcccctgac tttcagggct
     4261 atcttcatgg tctttgagga aaagtcctcg ccaaaatcaa aatacttata gcctataaca
     4321 ttgccctctg tgatgttcac gatacttctt gtgaagatat ccttttcggt gataaaacaa
     4381 ccacctttca gcacgcaggc tatatccgca ggataaggct tgaaagggtc aaggctgtct
     4441 tcaaaaccaa gcgaggtcat ttccactgtc ggtatcgttc cgtcagggag aatctctatt
     4501 ttctcaacgc aggcacgtct tgacattatg gtattgtttg tcattctgtg atagaaaata
     4561 taccactgac cattta
//
LOCUS       DAWEMR010000195         4575 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_6934_length_4575_cov_8.671681, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000195 DAWEMR010000000
VERSION     DAWEMR010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6934_length_4575_cov_8.671681"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3112..3121
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1220 a          995 c          967 g         1383 t
ORIGIN      
        1 gttatatctg catttttcgt cacacctgac aaagcatatg caagttatat cgacttccga
       61 acacttggac ttctgttctc tcttatgaca gtgaccgagg gctgcaaagg tcttggcatt
      121 tttgaacgca gtgcaaaatt tatcataagc aaagtcaata ccgcatttca gctttccata
      181 gtgcttgtgg ggctttgctt cttttcaagt atgttcataa caaatgacgt ttcacttctg
      241 acctttgttc ccctcactct caccgccctc tcaactctcg gtgaagcagg caacaagctg
      301 ctgatccctg tggtcgttct gcagaccatt gccgcaaatc ttggcagtat gctcacgcct
      361 ataggcaatc ctcagaatct ataccttttt ggtgtatcgg atatgaagct gaaagatttt
      421 cttgtactta tgcttccctt tactattgtt gcaggagcga tgctttttct gtggacgctt
      481 gcagtgtgta aaaagtcagg aaagctgtct gtcaagacag atataactgc tgaaaacttc
      541 gatacatatt cgactaaaga caagacaaag ctttcaggat acgtcctgct ttttgtcctt
      601 tgcctgctta cagttgcaag gcttgtccca tggcagataa cagccctctg cgcacttgct
      661 ttctctgcca ttgctgacag aaacgtgctt aaaacagttg attactcact gcttcttact
      721 ttctgcggat ttttcatctt tatcggaaat atgggcagag tagagatttt ccgcagtgta
      781 ctcagcagca taataaacgg tcacgagctt tttattgccg ttgtcgcaag tcagataata
      841 agcaacgttc catgcgctat actcctttca ggcttcacca acaagctgaa cgcacttata
      901 ataggaacga acataggcgg ccttggcact cttatcgcct ccatggcaag ccttatatcc
      961 tacaagcaga tatgccgcag ccacccccat atcaaaggca gatatttcgc atatttcaca
     1021 gccgcaaata tcgcttttct cgctgtaatg gctctgactg taaagatcgc atactgaaat
     1081 aagttttcta taccctgccc actttatctg cataacagtt ccgctaaatg cccacactat
     1141 ccgcaggagg tgtttttatg cttgaatttt ttgcaggaac gctggtgggc ggttttgtgg
     1201 gcgttgtgac aatgtgcctt tgtatcaccg caggcgaaag cgaccgtcac gatgaacgat
     1261 gatgttttac tcaggtaaac ttcgtgattt tcaccaaaaa agctgccgca gaatttccct
     1321 ttgcggtggc tttttttatg cctgtccgta ttgacaaata ctccgctttg tactataata
     1381 gtatcattgt actttagcaa gtaaaaacgc aaaagcataa aagctttaaa gtggcacatg
     1441 aagtaaaagg agtgtattgg attgacagca aggtggatag tattggtatt ataccttgca
     1501 cttatgatag gcataggtgt ttattaccgc agaaaatcgg caagcgtcaa tgattttgta
     1561 ctgggaggaa gaggtcttgg agcatggttc acagcctttg catatggaac ttcatacttc
     1621 tcggcagtaa tttttgtagg ctacgcagga aaattcggct ggaattacgg tgtagcttcc
     1681 acatgggtag gaattggaaa tgccgtcata ggctcatggc ttgcatggaa agttttgggc
     1741 agacgtacaa ggataatgtc aaaacacctt gaggcaaaga ccatgcctga gtattttgaa
     1801 aagagataca acagcaagcc tttgaagata gtttcggcac ttatcgtgtt cgtatttctt
     1861 atcccttata cggcttcggt ctacaacggc ttgtcaagac tttttgagaa gggctttggc
     1921 attccttaca aatattgtat aatcggcatg gcagttttca ctgcggtgta tgttatcatg
     1981 ggtggctaca aggctacagc actgaacgat tttattcagg gaatagtaat gcttgtaggc
     2041 attgcggcgg tagttcttgc agtgcttggt gaaaacggtg gccttggaaa ttctcttgac
     2101 ctgcttggtg atatttctga cgcaaatggc gacaagggtg catattcgtc actattcggc
     2161 cctgacccta taaaccttgc aggcgttgtt attcttacct ctctcggcac atggggactt
     2221 ccacaaatgg tgcagaaatt ctacgcaata aaagacgaca aggctatcaa aacaggcacc
     2281 atcgtatcca cattctttgc agttgtggta gcaggcggct gttacttcct tggtggcttc
     2341 ggcagacttt ttgtcaagag cgctgacgga cttcctgagg gcggctttga cggaatagtt
     2401 ccgactatga tggaaaagct cccttcactg cttttcggac ttgttatcgt tcttgtgctt
     2461 tctgcttcaa tgtcaacact ttcatcgctt gtgcttacat caagctccac tttaactatc
     2521 gaccttatca agcctataat gaaagaaaaa atggacgaga aaaaacagct tatcacaatg
     2581 aggatattca ttgccgtatt tcttatcatc tcagttgtgc ttgcaataat cactcttgaa
     2641 aatccacaga cagtaatttc aactctcatg tctatctcat ggggcgcact tgcaggagca
     2701 tttcttgcgc cgtttatgta cggacttttc tggaagggcg taacaaaggt ggcttgcatg
     2761 gcaagcttta tcgcaggagt aggcataaca gtgatacaca tggcatacta cacccttgca
     2821 ggtcacacag gaacactttg gggcataaac cttgcctcgc ctatcaacgc aggcgcattt
     2881 gcaatgcttt tcggacttgt tcttgtacct atcgttagcc ttatcacaag gcagagcgac
     2941 gctgacaagg ctcatgctga aaaggtgttc gagtgttata atgacagcga gatatgattt
     3001 cacatggctt acatacttga aacgcagaca gggacggact ttgttcgttc ctgttttttt
     3061 gggtgataac gccccttgca tcaattatgc acaaaatttg cacatatgat tnnnnnnnnn
     3121 nttctgcaag tgttgcattt tttatgcaac aaaacacccc tttttcgtgt ataagtagaa
     3181 agcgttttga tacttgcggg cacgtacaag ccctgtgact tggtgaaacc gaagggagtt
     3241 tctggagcag gctgtagaga acttgtgtca gaacgtgggc gtgcatttga tattttgtgt
     3301 atgcaaaacc tagggcatta tactcggtga caccccccga acgcaacttg aaaatcgaat
     3361 ataaaaaggt ggctaaaatg cactcacctt tgattttatc acgcaccatg acgatgcaaa
     3421 caaaaagttc cataaaacac cgttcaacac ctgaacgcat tttatgggaa ttttgctcca
     3481 ctgagattga cttgcaaggc attttatgat aaaatattga agataagtta caaaaacaac
     3541 atgacaatta tgcaaggggg gagaaaaatg tcgatacttt tattgccgtt caagatagtt
     3601 ttcctaataa ttgcgtttat actaaagggc gttctatact tgcttgcgtt tattctgaac
     3661 tttatttctg aggtcttagt cgctttgcaa tacatacttg gaagtgtctt tgtattggtg
     3721 gctatcggag gtactatcgt acttgtcaga aacattcaga atggctctct tacaggcttg
     3781 caaggcggtg tattgatagg ctttttatgg ctcatatcca tggctttcag catgatgttc
     3841 tatttaagca gtgcggcggc tgaccttttt gaaagcattg gcgattggtt aggggacacg
     3901 gctttgggct ttttctacta gagattgggc aaaaaagacg ccgtcaaaaa gacagcgtct
     3961 tatcattcac attttctttc ggcaaacacc taagccaagt tgaggtttca ctgtgcaacc
     4021 cctcccgaaa gctcaaccaa gcaacgctct tggcacgccc cttggtatca tcgcttaaca
     4081 gaactgccac gactatcaag tttacgtcag ttctgaccga aaactcctgt tttctgacaa
     4141 cgtttcgttg ctgatatcat tatatccaat tttttcgaca atgtcaagca catatcaaaa
     4201 aaccgctctg ataattcagg gcggtctttt tcatagcaag gctgattttt atagtcgctg
     4261 cggtcgacca actttcatgc agagggcgtt ttgcctttgc acagtgcata ctatactttc
     4321 aagcagcaat gaacttcaca gtttcggata cacgtcacca tttctaatgc gtccctcaag
     4381 tgtcagccac actaagaaaa gctacggata gatcttctca atactattat aatcaattca
     4441 atggtaaatg tcaagtcttg tcactaaaag gctatttcaa cttccgtgtt gatgttaaca
     4501 aacatttcac aatcaacaag gttttacttc tacaaaacat cccccttgaa tttatacata
     4561 aataatgtta tactt
//
LOCUS       DAWEMR010000196         4535 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7002_length_4535_cov_13.105134, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000196 DAWEMR010000000
VERSION     DAWEMR010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7002_length_4535_cov_13.105134"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1128 a         1118 c          954 g         1335 t
ORIGIN      
        1 tgacttagat caatacacag aacatctatc ataccgccgc cgtttgagcc tttcagagat
       61 attttctgca ctccgtttga agcgtctttt tctgtttcaa aggaacagcc tttcatcgcc
      121 tccgtaacta tcggggaaac atccgtaaaa ctgatatttg agttgaaaag ctctgcgata
      181 aaggtatcgg acttcacctg ttcttcatcg accgtacttt cagcctccga agaggcagcc
      241 gaatcgggta aggactcggc tgtagtggta attgctgttt ccgttgcggt ctgcaagggc
      301 ggacttatcg ttccgcaggc ggtcattgtt attgcgattg ccgctgaaag cacagccaaa
      361 tatatttttg ttttcatacg caaattctcc tttttaaacg attgaccaga tatacagcgg
      421 agcggtcagt gcaaacagta gacaaccgaa aattatggct atcggagtcc attcaaactg
      481 cccgtgtttg cggtaatcca tgtacgcaag ggaggcttta acaaacagca aaaccgccag
      541 aactacgtcg atcacgggga aaacgacatt gtttacgacc gacaccacct gtgaccttgc
      601 cgtagtccaa gtgcttgtga ccgctcccgc aacatcgccc gaagcgtatg ctgtagtcga
      661 catcatcgcc gcaaagcaca caacgcttgc gactgctgcg atgaccttct tttttgatct
      721 cattccgcat catcctctct gttatcaata attttcccta ccagaattgt acggctgttg
      781 ccctccgcag aagtgcaagt cgacagcatg atcagattat cattcgcaga aataccgatc
      841 tccttgtaaa tactcgcact gtttctgagc tgagtatccc attcctcaaa actgggatca
      901 gcaagataaa gtccgcttgt gtcagcagtt ctcgcaaccg caaagaaatc tatcagccaa
      961 acttcatcct cagtggtcag ccagccgtag ctgtggttca tgaaatattc ctcattctcg
     1021 taatgcggaa tatccgcaaa catcgtccca ttgcccatat tgtgaccgta gataaccgag
     1081 ttaaaatcgg tgaaatccga ggaatttctg tagtccataa acagcgagcc tgcatacaaa
     1141 tacgaaccgt cagtcgccct gtgagcataa taatcgttat cttccgactg catgatcgga
     1201 taatcgatat ttgtatcggg aatatacagc catgccacgg aatcgtcatg ctctgcggaa
     1261 agctgccgga cctgttcggt cagttcttct ttggtatctt cgggaactct gtctgccgac
     1321 ggtttttctg aatcgttatt taccgcctcg tcaagcgttt ctctcgctat cggggtggga
     1381 gcatagatca gaatatcccg ccaattgatt ttctgagcca catcgttttt cacaagtaga
     1441 taaattcccc ctccgagaag ggcggctgca cttatgacag ccgctatgcg aatcgctttt
     1501 ttattcatac ttttttcctt tctttgtaat gataagagcg gcaatgatca cgcttgcagc
     1561 tccgagcatt gcataagccg caaaatcgct gtggttatcg cctgtatacg gagtagtgat
     1621 tttaggtgta agctcgtcat acataatgac ttcggtcacg ctaccgtcag cgtttactgt
     1681 gaacttgatt tcatttgcta tctcatagcc ctccggtgcg atagtttctt tcagcgtgta
     1741 ctccttgcct gcgatcagct ttccttcgat gaaatgcggt tcgcttgatg aaacccattc
     1801 ctcaacaaca tttccgtccc tgtctataac ctgaagcttt gcaccgggca gttcctcacc
     1861 tgtggtgata tcctgcttgg aaatctgtac ctttgttgtg tcgtcgatca ttacaacgag
     1921 tggattgccg ttctccgaag tcgctgtggc ttcaacgttt tctacggtaa cattgccgta
     1981 aacgtcaact gtaaacttaa tatccgtagc aatcacatat ccgtcgggag ccgcaatttc
     2041 cttgagcgtg taatctccgg caggaagttc ggttacaaca tgatttgtgc cgtcggaagt
     2101 ccactcatca acgatttctc cgttagcatt ttcaagctgc atctctgctc cgataagctc
     2161 ctcgccgtag acatcaatct tagacaattc aacggtaata ggcttgttaa ttgtacgaac
     2221 cgtgatagtt tcgccgtcct cgcaaacagt tacgggatag ctttcatcac tgagaatata
     2281 tcctgtggga gcttcgattt catgaaccac atactcgccg taggggatct catcaaactt
     2341 aaaatatccg tttttatcgg aaacagcggt catatatgcg ctttcagaag taaattcggc
     2401 agtatcaact gcaaacagtc cgaacagagc gttttcaaga ggttcttcgc tctcgttcac
     2461 cttgataccc tcgatttttc cgcgtttcaa ttcatttacg aactgaccgc agtcgattga
     2521 aactgtagtt acttcctgtc ccatatactc aaatgtcaca agatattttt cgccgtttaa
     2581 aacgtagtgt tcatcagttg caatttcctg aacataatac ttgccgaatg ggattttttc
     2641 agcaatgaca gcagtcatat tttcgtcaag tgaaacctca gaaatgagac cgtccgcagg
     2701 aattacagta ccgtctgccg ccgtaatatc ttctgctgca aacaatccga agcttacgct
     2761 tgtatagcag tccttgccgt aaatgccaaa cagctcgtcg ttctccataa cctttgagag
     2821 actgatctca acgtcctgat aatcattgac aaatgacgta tttacggtat caagcactgc
     2881 gatttcctgt cctgcatagg tcagttcaac aaactgtgat tccgtattct gaacatagcc
     2941 aaacggtgca gaaacctcct tgatttcgta attgccaaga taaagcagat cgctttccgc
     3001 atagccgttt tcatcggttg taatttcagc tacaacgtcg cctgcgttgg ctctgattgt
     3061 gccgtcaagc gtaacgatat cctccgaagc gatcacctga aatactgcgc cggaaagatt
     3121 tccgcttgca aacacgggag tgtaggtggt cggatttact gtcatctcgc cgttttcatc
     3181 cgtgtaagcg gaggacgcca tagcaacggt tttaaaaata tcacctgttt tctgaaccga
     3241 aattctgcct ttctgagcgg tgttttcttt gtgaacttta atgactgtca aagcattttc
     3301 ttcctctgcg ttatcagcag aaactgtgaa gggcacgggc gtgctgtcca gaacataacc
     3361 cacaggagcc tgaacctcaa caagagaata ttcgccgtag ggaagaacct cgggagtgat
     3421 cagatatccc tcgctgtttg tgtaaaacac atcaatagtt gtgggagtag gatagctgaa
     3481 agtcatagaa atcttctgac cgtttgaatc ataaatctca aagcctgcgc cctcgtaagg
     3541 aatgacattg ccgctttcgg aatcgacctt taccacctta acatagcttt caaactttgc
     3601 attgttaatg agatagcgat aggtctgacc gttctcgctg atgttcacat caaaatcagc
     3661 tataaactcc gtaccttccc agccattggt ctgatgaacc gtgtaaattc cgtatggcat
     3721 atcctttgtc tgtgcaaagc cgttttcatc gcaagtcaga tgatcacgct ctgtttcagc
     3781 agcatttcca taactgccta cgctcttgag ataaacctca aattcagcgc cgacttcggg
     3841 cgtttcgatc tgagtcgagc cgtcgtccgt atgcttgatg actgagatat tgccctttat
     3901 tacgtcctca gtcacagtca tagaaatgag attactttca attgtgtatt tttccggctc
     3961 tgcgcctacg gaatgaacag tcatatcaag cagatatccc gcggagggag aaatctcctg
     4021 aactgtgtaa tttccgcaga catactcctt ggacttgaaa tatccgtttt catctgtggt
     4081 gtaggtgtca ataagcgctc cgtcacggta aatgccgtaa actgcgcctg caagagtacc
     4141 gtcgccctgt gcggagacca tttctgcgtc ctgctttgtt acctcaactg taaacttttt
     4201 gaggatattt ttgaatgtct tagttgtggt tgcgtcagca gtaagagttg ctgtctgatc
     4261 agcaggaatc acatatttta tcggaacgtt tttctcgctg attgtgtagg ttattttctc
     4321 gttattactg tcgtagacgg gaatattttc aagcagagcg ataccgttct cacctgtgga
     4381 aatggtatag gttttaccat ttcctgtgac agtgaattct ctgccgccgt tctctccgtc
     4441 ctccgactgc ttgttgatct taattgaacc cgttttaagc tgattgttga acttcacatt
     4501 cacagtcaga tcaacatctc cgcttgtcag cgtaa
//
LOCUS       DAWEMR010000197         4462 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7131_length_4462_cov_11.940095, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000197 DAWEMR010000000
VERSION     DAWEMR010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7131_length_4462_cov_11.940095"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1383 a          795 c         1154 g         1130 t
ORIGIN      
        1 ggctcaacgc tgagatttct catacctgtt gcttgtgcgc ttggggtgga tactcagttc
       61 atcggcaggg gcaggcttcc gcaaagacct atcacaccat atcttgaaga gtttccaaag
      121 catggcgtaa gctttgaata tgacaacact atgccttttt ccactaatgg gaagctcaca
      181 gggggcaagt tctatgttga cggtggaata tcctcacagt ttatcacagg gcttctgttt
      241 gctctgccgc ttcttgagga ggacagcgag ataatactca cttctcattt ggagtcaaag
      301 ccatatgttg acataactct aggcactttg agagagcatg gctgtcaggt ggaggaaaca
      361 gagaatggat attttgtaaa gggcggtcag cgttatatgc ctttcagcgg cagagttgag
      421 ggggattatt ctcaggcggc tttcttcaag gtggcaaatt ctttgggcag tgatattgat
      481 atcgcagggc tttctgaaaa atcttttcag ggagataaaa aaattgttga aatatgcgag
      541 aaaatagtgt ataataaaaa tggcggtttg cagccttttg acctcgactg ttcggatata
      601 cccgaccttg tacctgttct tacagtactt ggaagcttct gcgagggaaa aagctatata
      661 cgaaatgccg cacgtcttag gataaaggag tgcgacaggc tttcggctat ctcacagtgc
      721 cttaacgcaa ttggcggaaa ggtaactgag cttgaggacg gattggagat agagggcgtt
      781 ggctcactga atggcggaga agttgacagc ttcaacgacc acaggatcgc aatgtcaatg
      841 gcggtggctg ccacaagatg tacttcacca ctgaagataa acggtgcgga gtgcgtcagc
      901 aagtcatatc ccgatttctt tgaggtatat gagagcatcg gaggagagat aagccgtgag
      961 ccttaacgtg ggctagtaca aaaatttgta ctgatctggc gggtatctgc cgttatttat
     1021 acaggcttta taagctgaca ggaggaagta aaatgtcatc aacatggaac aacaatatag
     1081 gctttaccgt tttcggtgag agccacggcc ctgcaatagg cgttgttatg gataatgtac
     1141 cgccggggga gagcatcgac cttgacaaga tatatcaatt tatggcaaga agagcgccta
     1201 agcagaacaa gacctctaca cagagaaaag aaaaggatat gccgcagata atgtctggct
     1261 atttcaacgg caagactacg ggtactcctc tttgtgcgct tatagccaat acagaccagc
     1321 attcacagga ttattcaaat ctttcaaggc ttgcaagacc aggtcacgct gattatacag
     1381 gcgcattgag atacagaggc ttcaatgatg taagaggcgg cggacatttt tcaggcagac
     1441 ttactgcgcc cctatgcttt gcaggagcag tggcaggtca gatacttgaa aaaagaggta
     1501 tctatgtggg cgctcatatc gcagaaatcc atggcataaa ggataaggct tttgacccta
     1561 tcaataccac taaggatatg ataagggaag ttcgtgagaa agagttcccg actattatcg
     1621 acgttcaggg caacgaaatg ataaaggaga tagacaaggc aagaaaggat aaggacagcg
     1681 ttggcggaat tatagaatgt atcgctatca atgttccagc cggtatcggc tcgcctatct
     1741 ttgacggact tgaaaactct atagctcagc ttattttcgg tatccctgcg gtaaagggtc
     1801 tggagtttgg tgcaggcttt aaggtcgcag aaatgacagg ttcggagaac aatgacgagt
     1861 tctatgtaaa tgataagggt cacgttgtga caaagacaaa caatcacggc ggaatactgg
     1921 gcggaatagc ttctggtatg cctatctcac ttagagttgc tttcaagcct actccgtcaa
     1981 tagcaagacc gcaggcaact gtggactatt cagctatgaa aaatgaaact ctcgaagtaa
     2041 agggcagaca cgacccttgt gttgttccaa gagctgtgcc atgtgttgag gcggctgtta
     2101 atatagcttt gctcaatcat atgcttgaat acccaaattt ttagagaaat ttccaaagga
     2161 ggattgatag cttggtacaa aatcctgagg ctcatatatc agagaacatg aagaacattt
     2221 ccgacgtgta tgaggttcaa atacttctgg cttattttct gggtcagata aatcagcttt
     2281 gtacgcctaa tcagcttact gagatagcca cgggcgaagg cgttgtgaat tattttgtgt
     2341 acactgaggc tgtcagcaga atgttggaaa acggcacgct ggagcttgcg gaaatagaag
     2401 gcacgaatta ctatcggctc actgaaaagg gcattgaggg cgctgagaaa ttcaaaaaac
     2461 ttgtgccgaa aagttttaga gataagatat atgcggcagg tctaagactg ttcgtcaagc
     2521 ttaaaaacga tcgtgacgta agctttgata taaagcctgc aaagcgtggt tataacgtgc
     2581 attgccgctg ctgtgacgat gatatggtgc ttatggacat aactctttac gcacctgatc
     2641 aggagcaggc aaattatata aaatcgaaaa ttcagatgaa tcctacagat ttctattgca
     2701 gagtggtgga ctatataatc gagaacgagg aatatgtgcc gtctgctgca gaggagcagg
     2761 gattgttcta aagaaagtgg agccgtctgg gcgtatgctc aggcggcttt tgaaaaaatt
     2821 tttatttttt tttgtgacat tttgggagag aataagtatt tattataaag gggtgatcaa
     2881 atggacgtca atttgaaaaa tacgccttca gagatagaca ggcttataga taaatataaa
     2941 agtaccgttt atggaatcgc tatagcacgt cttggctcaa aagcagatgc agacgatgtt
     3001 tttcaggaag tgtttcttgc agcttatcga aaggggataa cagtcaagga caatgatgag
     3061 gagaagttct ggcttatcag gactactctc aatcagtgca aaaagataac attgtcaagc
     3121 tggcgcaggc gtacagttcc ccttgaagaa agaaaggaag aggtaagctt tttcagaagc
     3181 cgtgaggaaa atgatgtttt ctgcgctgta acagaactgc ctgaaaaata cagaatggtg
     3241 gtctatcttt actattttga agaggtgtct actgatgaga tagcaaagat aatgaaagta
     3301 cgcagtggaa cggtgagaat gcagcttaca agggcaagag aaatgttgaa agaaaagctg
     3361 aaaggaggat atttctatga ataaggaatt tttcagaggc atgaaagagc agatagagcc
     3421 tgatgaagag caggtcgagg agctgaaaaa taagctcaag aatactgcgg cggtaagcag
     3481 gaaacctatg tggataggcg gagtatgtgc ggcggcactg tgtgtggcag ttggagcagg
     3541 tctgttttta tccgacagca aaactgataa aaacagcata aagacaagcg gcagttcatc
     3601 agtgcagtac aatgacagtt ggtcgcctga ccttataaat agaaatggga acggctctga
     3661 cggaaatcag acagtagctg gtggcgaaac agaggaaagc attgtcgaca gtacacatga
     3721 ttcaaagatc gacaacggca agacggacga tacgcccaat ataaacaaca atacgggtgg
     3781 aaatacgcac ataacagaca atgaccgcat aagtgacaac aatggcggaa ttatttcaga
     3841 caatagtaat acagaaaata cagatagtaa tgcaaacgac agcaaatcgg agaccacatc
     3901 gtccaaaaat gaaacggaag accctataga gttcgatgaa aaagaccctg tataccctga
     3961 gatatatgtg caggacgaaa ggctttttga tatgacatta aagggcaggg catttgcatt
     4021 ccctgcaaag tatagtgatg tagcggatat gggtctttgg gcggcaggtc taagtggtta
     4081 ttctgagagc gttgacccga aaaattatgt gatagcggaa gatgatatgg aaactttttt
     4141 cagtaatggt gcaacaggat attcaggaag catgacaatt gacaataaca cttatgagga
     4201 agcgaaagca agcgattgtg atgtcatggg attgtttttg gataactgtc ctgagttctc
     4261 tataggcgga ataaagactg gatcgactat agaggaagtg aataagacat tcggtgttaa
     4321 ttatactaat gaatgcagaa cgcttactgt acattctcag aacggagctt ttacagacag
     4381 caagggtact tttactttaa cagttgtatt tgcgttcgat gacggggtag tttatagtgt
     4441 aggcgagtca aagatatatt cg
//
LOCUS       DAWEMR010000198         4437 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7180_length_4437_cov_11.302373, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000198 DAWEMR010000000
VERSION     DAWEMR010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7180_length_4437_cov_11.302373"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2312..2321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1155 a          960 c          819 g         1493 t
ORIGIN      
        1 gtaagataac cgcccctaca aaaaaggtta cgttatctta cctcaacaaa atttgctccg
       61 gaatatccgt cgtcaacata gaatttacag tttaagaaac catggtcatt cgcatatttt
      121 tgcaggatga gtttctgatt ttcaatagag accgactcac cgtccctcat atcctcctgt
      181 gataaacggc agtataacgc agtaattttt cgattcgaat cagacatatt tatccatcct
      241 ttcatagtct gtaaattttc aaaccgaata ggatacagct acaatatatc actatccggc
      301 ttgaaagtcc agaaaatttt ttgataattt tatctgccgt taataagata agcgaacagt
      361 tccgacactt tttgtggctt gtatccttca tctgctctgg gactaacgga taagattttg
      421 taagttactc catcaatcac agtttcgatg tgcgagtaat catgtcggcg tcggtactga
      481 tttgaatctg gtttctcctc tatttctttg tgaatattct gcataaatca ctctccattt
      541 cgttgtcttg attgttgtta ttcgtgtaaa caaatcagtt cagtcctttc atatttaaat
      601 tttgcggttc gtattcgtta cgcaccatct tagccttgct gaaaatcaac agcgttctcg
      661 taatcgataa ttccattgat cttccgtact tcgtttacgc acatagcgtg cagactttgc
      721 aatgtttcat catcaacatc atttatcgcc gcagtcatat gcgacagctt tccaagctcc
      781 actgcgactt tgaaaagcag acgggcaagc cgctgttctg taccttttat ctgtgccttg
      841 accgtttccg taatcagtgg ggagaggtag tttacctctt cctgctgacg aaggtagccg
      901 atgtaaaact tcacggcttt ctcaataaat tctgttttcg aggcacaatt atcctcttta
      961 tactcctgtt cgatcttttt aattgtttct ggctgaaaat atacagccat tttttctttt
     1021 gccatgaaac aactcctttt tcttttacga ctcctgaaaa tacgccgatt tttcgcacat
     1081 ttctgcactt acgacccctg attttcgtaa aaagccgtat tttgactcca tgaggcgacc
     1141 gcctgaaata tgcacaagac ctgtgtgaag actgcgtatt tacggcacat ttgcggtcag
     1201 ctttgctgtc cgctgtgagc gtcatggcgg cttcagacgc catgacttta acctgcaatg
     1261 aaatatttca ctttcttcgt catgcggcga atcgggaatt tgtgcataac caacatatca
     1321 agttcccgat gtgcttttta actgatttca gaattgctca tttatccctt cgtacaccgc
     1381 ttacaatcgg cgtgtgtggc ttataccgtc tacggtggta tgtttaccct actgcgagtt
     1441 gggagggcaa atatcgccgt tgtgttgcgg ttaaaattaa ttacactttt ttgattattg
     1501 aacatctcca ttcttattcg cagaagaaaa aaggcggatt gtcaatcagc gaatcaatcc
     1561 gccgattcta ttcgtcattc atcaccaatc aatctttgat aactacatat cattctttct
     1621 tctgctatta ataatattag ttagtattaa tccttaaagg catagcactg tgacacggag
     1681 ggtatcgcaa ttattatttt tgtttgcccc acaacagtac agttcctgtc agaaaatctt
     1741 gtctgactta aggacgtatt ccaagcttgg ttacgcttct gctcctcctg aacgatcttc
     1801 gctgtacttc gccgcttggt atctctccta cgtgggattt tgttgtcaag atttttttgc
     1861 cttcatactg tagccgatga aaagatgctt tttatgcgtg tgtgaaaaaa ttttgtagac
     1921 ttgcacaaaa tctgaatgga ttatttatag agttccctct cacttgtata cggaaaggag
     1981 tcgccgtttt gacaaccctt aattcataaa gtttgtaggc ttgaacaaag tcgtaacaaa
     2041 ttttttgtag agtttgctct cacttgtata gggtaagaag ttgaggtttt acaaccattt
     2101 tgacagcaat tctttaaaag tttgtatgat tatacaaatt cggggcggac gatttagaga
     2161 gtttgcatat tggttaggta tccctataga aaccttttcc cagtgaatca gggtggtagt
     2221 tggctcgttt atgaagagtt ttgcgttgac agtaactctg aaatgttgac agtaaaacta
     2281 ccacttgatt tctaccgtca cacctcgcaa annnnnnnnn naggattttc aatggttttc
     2341 agacttttaa agccaaactg ttgacagtaa cttcgcccga ggttttactg tcactactgt
     2401 cacgctcacg gtcgtaatga agaataatca gtctgccgtt tcgtgtccgc ttgtacagaa
     2461 aatcaattcc atactgtctg agcgtgtaac cgttctgaac caaatctctt gtaactctgt
     2521 tcggatagaa tgtttcattt gaaatacttt tcagtgcggt taccaattct gaagccgttc
     2581 ctgtgaaatc aatctttttc tgaatgtaaa catacaatgc ggcgagaaaa atattgtctt
     2641 tgcttttcga aacagttggc tcatctgtga ctatccactt tttcaaatcg ctatcaaact
     2701 gcaagcttat ttctgcgttt tcaatatcac gacctacgca gtatagcttt gcatttcgac
     2761 tgccacgctt tgtttctaca ataaccatgc tttcgtcaac gcatccgctg attcctgtcg
     2821 agcccgaaat catattgaac ggatcgctgt ccgaacactt tcttgtgtga tgaacaacta
     2881 cgattgcaat ttcaagcttg tccgcaagat tttttagaac agataactct ttgtagtccg
     2941 agccataacc gttatcggtt gactcacgaa tcatttgcag agtgtcaatg aacacaactt
     3001 ttaaatcgct atgctctttc ttgaaattct tgatctgctc ttcaagtccg ctgcctattg
     3061 attctgacat gattacaaaa tgcaatcgct ctgtcggctc atctgtaatt tcatagagac
     3121 gattttggat tcgtacatag ctgtcttcca aacacagata gagcgcagtt ccttgagccg
     3181 tttcctgacc taaaactttt tctcctttcg ctatcgacaa gcacatatca agtgccagcc
     3241 atgactttcc cacttttggt gcacctgcta agatgtacag accttgcgca agcaagccat
     3301 ctacaacaaa ctcgataggc ttgtacacag ttgtcatgat ttcttcgcag gactttgagt
     3361 tgaatttctt caagttatca agcctcctct tctttttttc tttttacttc tttcccacaa
     3421 cagtacaatt cctgtcagaa agccttgtta gacttaagga cgtatttcca agcctggtac
     3481 gtttctgctc ctcctgcacg atcttcgctg tacttcgccg cttggtatct ctccttcgtg
     3541 ggattcaatt gtcaagattc cttttcctca tactgtagcc gactaaacaa agtgtttggg
     3601 caacttctga aaaaagtttg taggtttgca caagttagct catatgattt cggttatttt
     3661 gactaccact gctcttccgc ttgtatatgg gtagaagtcg ctgttttgat acccctttat
     3721 ctaaatgaat tcataaagtt tgtagggttg aacaaagtcg taacggatta tttgtagagt
     3781 ttgctctcac ttgtataggg ttagaagttg aggttttaac aacctttttg gcagagtttc
     3841 tttaagattt tgtatgattg tctaatagac agagcagtga tgacttttgt tttttgcatt
     3901 atcacaacac gatgcggtag gacgtgaatc acgacctacc gtaaaaagta aggtggtaaa
     3961 catctatgat ccgacataga aaaaccactg ccgtgttttc cgacagtggt tcgagagaat
     4021 gattttatta tgttcaaaaa atgatatctc ttgccgtgaa gatttactag gctaaataat
     4081 ttgtcacagc ttgcttcttt aaaacaggag cggggtaatc ccctcgtaca ccacaacaat
     4141 cggcgtgtgt cgcccttttc ctcagaggat ggtatgttta ctctactgcg agttagggac
     4201 aaatatctcc gttacgctgt gtactttcga gagcaccttt cgttgacagt aactctgaaa
     4261 ttttgacatt gaaactcgtc tttgatttct accgtcacgc ctcacaaagc aaagattttt
     4321 aagagttttt aagcttttga agcaagctga ttgaccatag aatttttatt gtcagtacta
     4381 tcactctgac gatcataatg aagaataatc agtccgaaac ctcaaactca tctgaac
//
LOCUS       DAWEMR010000199         4392 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7260_length_4392_cov_11.445008, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000199 DAWEMR010000000
VERSION     DAWEMR010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7260_length_4392_cov_11.445008"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2485..2494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1243 a          994 c          740 g         1405 t
ORIGIN      
        1 cctttttgaa aaatctgcaa ttatgttatc tagaccttca attattgtac ctctgccaaa
       61 atccatttgt tgttgaagaa cataaaataa agcattcgca aatacaacag cacaattatc
      121 ataatcatca tctactggca taattacttc acatgaatta ttaattaaat tcgaataatt
      181 tgacagtccc aaagatgcga atgttaaaca atctttaaac gttctgccaa atcccaaaat
      241 ttgtataacg tattcgccgc ttgtatattt atctacacct atatattttc ctaaaaacaa
      301 ttcataatgg tcaagtatac tttcacctaa actcattcag caaaatctcc tttcactcct
      361 tcaaatatat gttttatatt gcattcatga cactgaactc tcaaatctga attataacag
      421 tctaaaactt ctgttcttgt aggagttgtt tctaatgatt gtaattttac ctttctctca
      481 gcccaagtct ctggataatg atcaaggtca taacctattc tatcaaccgg accattcttg
      541 gtatttattg ttattttatc aggtatttct tttccacaag ttgggcattt catttttccg
      601 ttagagtttc ttggtgcctc actttctgct ttttgtcttg cactgcgtct aaatgatccc
      661 cttgcatata aatcacttgc attatgtacc aacacgctaa aaccgctaac gtgacatgta
      721 tgaaaatctt ctacctgaaa attatacacc tttacgggtt tatctgtcaa ctcaacatca
      781 aaattttcaa ctaaaagaac atttccgttt acatcaagaa gttcatcacc aactattagt
      841 tctcctgctt tgataaagcc ttgatttttt acatagaacg ggtggtcaac agttgtaacg
      901 atttcttcac cgttcacagt gagatgaaca agcgttgtta cctctcttat gtaggtttca
      961 agaacagttt tcggagaagt ttccattgtt tcagggtctg ttgaaatgac tttatctcct
     1021 gactttatct tttctatggc aagcaaacta accgccttgg cattacctaa aaactcacct
     1081 gccgctccaa gtccgccaaa acctagtcat gcgatgtgta gaacatcttc taatctaatt
     1141 taatctgaaa tagcaggtgc tctttccctt gccctctttt tttagttctc ctgcggcaac
     1201 aagttttctt agtgcacctt cgatagaact gtcactgaga gtaggacaga gttcccttat
     1261 atcctgcttg ttaaatcgtc ctattttgct tttgcttgcc attctgacca tctcaagtgc
     1321 aggcagtttt tcttgaacaa gcgagatacg ctcttcaaaa tctttatacg atgcaagtat
     1381 cgtgcctaaa atgtacttta taaatggcaa agcatcctct ttgccctcgt gccagcccgt
     1441 ctgcgactga ctcatagcac tgtaatacag atctttgttc ttagagatct ttgcctcgag
     1501 cgatatatat ttgccaatgt aaaagccatt ctgatacagc agaagagtgg taagcagacg
     1561 gctcattctt ccgttgccgt cattaaaagg gtgtatacag agaaaatcgt ggataaatat
     1621 aggaataatt ataagcggct caacctccaa attaccgata acacgattgt actcttcaca
     1681 tatcctgtca aatgcctcag atgtttcatt tggcgcgagc gaagtaaaca gtatttctgt
     1741 atgtccatcg gggtaagccg cactgatata attctgcacg ctttttgttc tgccagcaag
     1801 cggattgttc atatgactat aaagcatttt gtgaagctgc agaatatagt tctgagttat
     1861 cggaatagcg tcaaagcttt cgtgtataac gttcagcacg tctctatacc ctgcgatctc
     1921 ctgttcgtca cgatttcgag gagtagtctt ttcttcgacc aactgtttga tcctcgtgtt
     1981 tgttgttacg ataccctcta tcgcatttga agcttcggta ctttgaattt ttgccatctc
     2041 aacaagcttc tccatctctt gcggacgctg tttcagatac tgctcctgtt ttccagcctc
     2101 tttgtatatg gccgctataa gtccgagaat accagaatcc cacttttgtt ctttaaaagc
     2161 agaataatta aaacttctca tttccttacc tccattattt tccattagac tatatcataa
     2221 aataagggaa atgtcaatag aataagcaaa tttgtcctta atattatgcg cgaaataagg
     2281 gaatatatca aacagtattc ctctactgta taaaagagct ggtttctcta ctcttatgat
     2341 atctatagca ttggggaaca tattcgtaat tttacatcat aaattgttcc ccacgtttct
     2401 tgttttggtt atgaaaaaag cgcctatcag tgatagatgc taatggaatt tgataaattt
     2461 atatggctat ttcaacttct gtgtnnnnnn nnnnccacat actttcacag cgtgcattat
     2521 catgacatct tccgccggca ctgttcatgc tttgaatgat cccatatttc ggctatttca
     2581 acttccgtgt tgataaatgt aaactttttc agaaaacatg ttgtaatata tgacaatttg
     2641 gaataaggct gtagacaaat ggggtgtttg gcaagctgca cagcatatag ctaagaaata
     2701 tgatgacgga tatcttcgca agaaagaata gcataaacaa aaaataagct gctacagaaa
     2761 aaatctgtgg cagcttattc tattcagaat agtcctaatt ttaacctatc aatattagct
     2821 cctgatatct gtttgtacga atccatactt actcagtcta tgtcacctta ctccctcaat
     2881 gactccctta tccatttcat gcacagtatc gcacagcact gagatgtcct ccgcagagtg
     2941 tgagcatatg agaatagtct tgccctgctc tttgtagctg agcaaatact ctctcatttc
     3001 cttaacgccg tccttgtcaa gaccgttgaa aggctcgtca agaatgagta tcttcgggtt
     3061 ttccataatg gcttgtgcaa gccccaatct ctgccgcata cctagactgt atttgcggac
     3121 gtggcgcttt aaatcagggt caagccccac ctgcttcatg ctgttgcgta tctcttcttt
     3181 gcctatcttg ttgttcagcc ctgcaagcag ctttagattt ttgtagcctg aatagtatgg
     3241 tataaacccc ggtgtttcaa tgataattcc catatctttc ggaaagtcaa cgtcctttcc
     3301 gaccttcttg ccgtcaactg ttatctcgcc cgatgttggc ttgataaagc cgcatatgca
     3361 cttcatcagc atcgttttgc cgctgccgtt tctgccgata agtccgtgta tcttgccctc
     3421 ctcaaaggac acatttatgt gctttagtat ctccgttttg ccaagcgtaa ggcatacgtc
     3481 atttacattg atgatatcca ttttgtcccc ctaacttgca acctccgtca cattatcata
     3541 attaaactta ccaatggcta tccctccgaa aaccagcaga accgcaataa gtactagcag
     3601 ataaataaca gaaaagctca tcggcattat tggctttttg aaatattttg tgtaatgcag
     3661 ccaagttatt gagtttgcca ttggcattgt ccacataaga tttgtcctta tagcactgaa
     3721 cgccattcca agggatatca ttccgccgca tatgataaag cccaatgtct ttcgcttgaa
     3781 tagtgaaaac accagcagta tcagccctat tatcatcaaa tacgccgcca cgagcagata
     3841 gcttttcacc gccgcctcaa ataccgaaag ctgattgtag agattttctg gaagcagcgc
     3901 aactccgaaa tcgcctcgcc tgtcggggaa cattttcaca aaacccgtgg caacattgct
     3961 ccagccattg taccaaaaac cctttgaaag cattggcaaa actgtaccga gaaatattac
     4021 cgcaagatat gatactatca taaaaacaag tttcaaaacc tgccctacaa accagttcgc
     4081 cctgccgata cgcacgatat aaaacacagt attggtgtcg actttcggat agtcagcaat
     4141 gagggtaaga aaaactatgg ggattataag tatcagtatc tcagaattcg ccacagctat
     4201 aaatggctct agtatgttaa gcggctcacc cataagctct gcattgttag tcagcggaga
     4261 aactgcaaaa ttgtttatga atatgagcag caccgcaact atcaccattc gtgcgtcgca
     4321 gacccatttg acaaattccg tcacctcaca ggaaaaggct tgcttcaaac taaaattttt
     4381 catcgcttac ac
//
LOCUS       DAWEMR010000200         4375 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7288_length_4375_cov_11.953935, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000200 DAWEMR010000000
VERSION     DAWEMR010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7288_length_4375_cov_11.953935"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1374 a          865 c         1022 g         1114 t
ORIGIN      
        1 gtgcagagcg acggattcta taccgtggat atccctgatg ttgagcttga cgagggtgaa
       61 agatattctg tcgtgatgac attcagcggt gatgacggct caggttatgt gaccgtttat
      121 ggctattcag acggggtcat gaagcctggt caggcgtata tttccaatga cggcgacagt
      181 tggaccgacg ttaccgacaa ggacgcttat actggtcagc ctataatatt tgcctatacc
      241 gatgatatag ataagtctga taaaagcgag cttgaaacac ttgtggcaaa gtatgaaaag
      301 cagagcggtt atgagcgtga ggtcaacaac ggcaagaaag ttattgcaga tgaaaacgct
      361 tcaaagaacg atatcaccaa tgccaagctt cttataaagg caaaggcgaa agagataaag
      421 gaacaaagcc ttgttataaa gacagcgacg gattggaaaa acttcgcaaa gcgtgtatca
      481 ggcggtgaaa gctttgcagg aaagcgtgtt gtgcttgaaa aagacatcga cttcggtggt
      541 gcaaagatat cggcagtagg aacggcttca aagccttttt gcggatattt tgacggaaac
      601 ggacacgttc tgacaaacgc tgttatccaa aacaattctt atgcaggtct gttcggctat
      661 gctgaggaca gagcgcagat aagagatctc agtgctgaga aaataactgt cagcggtaag
      721 tacgcaggcg gggtcgttgg tgaatttgat gcggtatcta tccaacgctg tacgttcagc
      781 ggaactgtca agggtacaag ctcggcaggc ggaatcgtgg gcaaagcagg tggtctgacc
      841 ctgacagagt gcgcttcaga ccttgcaaag tcaagcagtt caaatgctaa agctatggtg
      901 ggcagcattg ataatgacgc tttttattat ggacagcaat ctgccggttg ctggtataat
      961 agcaactttt ctgacagcaa ggtaaagaaa acggctttgt ctaacgttac aagctctgca
     1021 gcttattcgc tgaataccgt tggcggaaag cagacagaca gcggcaggtg gacaatggca
     1081 ggcggaaaag caaggctaac tcgtcctggg gagcaggctt cccacaagat aacatttgaa
     1141 gctcctgaca gcaagcatta tgcctgcact gataaagacg gcaaggtgaa atatcctgct
     1201 gtcaaagtgc aaagcggtta tgctcttaaa tggtacaaga acggaattgc tgtcgatgcg
     1261 aacacagttt atactgctga cagcacagta aaggcaaagc ttgttgaagc aaaatttgtc
     1321 actgtcgact atgttttaaa tggcggcacg aacagcacag gcaatccaga atacctcagc
     1381 aaaggcgaaa gcgtgacgct ttccgagccg acaaaagagg gtgcagactt tgcaggctgg
     1441 tatgataacg ctgcactttc aggtgaaaga atagaaagca tctcatattc aggcggtgca
     1501 aagacccttt atgccgcatg gaagccttat acatataacg ttacgtttgt ggataaggac
     1561 ggcaaggtga taagtcagca gacagtggaa tatggtaagt ctgcaaaagc tccgaaagcc
     1621 cctgctataa agggtctgcg ttttacaggc tgggataagg actataaaaa cgtcacagag
     1681 gatatgactg tcacagcgga gtattctgac agcaagctta ttgcagactg cgagatatca
     1741 ggcttcaaga acagcttcac atacacagga aagaatattg aacagcctga cgtaacgctc
     1801 acatatgagg gcaaagagct taaagagggc gtggattaca ctgtttatta taagaccaat
     1861 cttaacgcag gacctgcaaa aatgaccttt gttggaaaag gcgaatacag aggctctgtt
     1921 gcaaaaagct ttactatcca gccaaaggac gcttccaagt tcaagattta ttatgctaca
     1981 ccgctgtcgt tcaccggcca ggcgataaca cctgatgtgc ttataaagga cggaaataag
     2041 accctagagc gttatattga ttatatggtg atgtatttct caaacgtgaa cgttggcacg
     2101 ggcaagataa cagttgaatt ttacggaaac tataagggca agcagaccgt ttcttttgtt
     2161 attaatcctg caaaacagca gatacaggtg cttgaaacaa gatacaaggg cttttttgtg
     2221 gattttgtgc agaaaggatc tgcaacaggc tatgaggtcc agtatggcac aaaatctgat
     2281 ttttcagatg cttccacata cagatccacc accaacaaga cggataagat gacggtactt
     2341 ggattaggga cttcaaagac atattatgta agagttcgct cttatactgt caagggcgga
     2401 aagacctatt acggcaattg gtcagacgct aaaagcatag tgacggcaaa gactaatttt
     2461 gcaaacgcaa aggtcagcgg tatttcttca aaaagcttta ccggcaaggc gataacgcag
     2521 aaaatcacag taaaatacgg ctcaaagacc attaaaaacg gcacagacta cactgtgaaa
     2581 tattctgcaa acacaaaggt cggcaccgca aaggtgacta tcacaggcaa gggcatttac
     2641 ggcggagtta tcaccaaaag ctttgctatc tatcctgcaa agcaggtgat acagaagctt
     2701 tacggcacat atgcaggctt ctttgtagac tatgcacaga aaggctctgc cacgggatat
     2761 gaaatatcat actcacagcg ttccgacttc aagggagcaa aaagcgtaaa ggtaacaaac
     2821 aaccgcaccg ataaaaagac agtttcgggg cttgcaagat atcaaaaata ttacgtccgt
     2881 gtaagaagct acactgtcaa aggagaaaag acttataacg gagcgtggtc cgataaaaaa
     2941 tgggtctata ctcaatgata tgacatacga taaaaaatac ccctgatcat tttctggcca
     3001 ggggtatttt tttgctcaga aaagttctgc tttgtacttg atagaatcct cctcagttat
     3061 ctcacggata actctgcatg gatttcctgc cgcaagagaa tttggcggta tgtcacgggt
     3121 gacaacgctt cctgcaccga taacacagcc ctcgcctatg gtaactccgc ctgtgatgac
     3181 aacattgctc gctatccagc agttggagtg tattgtgata ggcttgccgt attcatcatc
     3241 ataaacagtg ccgtcagact ttttcttcat gttacgctcc tgccatctga atgggtgaac
     3301 aggtgtggca atggtgcagt tgggaccgaa aaacacgttg tcgccaatgg tcacagggca
     3361 ggtgtcaagc actgtcagat tgaagtttgc atagcagcct gagccgaact ttgtgaaaac
     3421 gccatagtca aaatacacag gaccttgaag aaaaaatccc tcgccatggt cgggaagaag
     3481 ctcgtcaagg atagcgtttc ttctctcatc atcttcatac aaactgctgt actgctgtga
     3541 aagcttgtga gcctttgtcc tcaattctgc aagctccttg tcggaggagt catatatctt
     3601 gcccgcaagc atttttcctt tttcagtcat tataaaacgc cctttctgcg tgtagtttca
     3661 tttgattttg ttgagagtat agcacaagtt tcgggctgtg tcaatagttt tttgggggtc
     3721 gagtgataga ataaaaaatg gtatacaaaa gtggagaatg cttatgggca aagcactctc
     3781 cacttgatga gtatactatt taaaaagctt ggcaggatag aaaaagttat aggtaaaatc
     3841 cctgcatata atttgtgtaa ataaatgtgt cctcgtcatc agcggtattg tcaccagtgg
     3901 gcttttttct gccactaaca caagaagcca aaattatcag accgcctaga acaattgcaa
     3961 cgctcactcc aagggctacc aaaaccgcac agaaaatttt tactatcata aacaccgctt
     4021 caatcatatt tctcatactt cgccataatt actattatga ttatatgctg gttttgcctg
     4081 ataatcaata aaagccgtgt aaaatgcaga taaaaatcaa gtcagctttt gtctgcatca
     4141 aaaatcttgt agtaaaaata tttttcaaaa cgtttcatga tttaacaatt ttaaatgaag
     4201 aaaattatag ctttcagctt agtataaaat tgcacaaact gagctttttg taaatggtta
     4261 gcattgtata ttacactgaa aatgtacgat tcttgaaaaa atatacataa atgtggggtg
     4321 aatacttgac tagatgaaaa ttttgtgata aaattattaa agtgaataag tgtat
//
LOCUS       DAWEMR010000201         4372 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7296_length_4372_cov_7.566829, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000201 DAWEMR010000000
VERSION     DAWEMR010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7296_length_4372_cov_7.566829"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    420..429
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4059..4068
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1437 a          795 c          922 g         1198 t
ORIGIN      
        1 taagtgtaca tttaattgta cttgtatcaa aagtacgatt gacttttgag ccttgccgtg
       61 ttattatata aatatcaatc ataacggagg ctcattcaat ggtaacagtt ataagcggtg
      121 gctctcacag tggacgtgac aaagttttca tagataagat caagtctgct ataaaaaacg
      181 gcgagcaggt catggtcata ataccagatc agttttcttt tgaatatgat aaaaaactgt
      241 atgaacacct tggtgcggca gatttcaacg tcatacagac tgcaggcttt aaccgtatcg
      301 ctgagttcac agagaagctt tatggcggta gggtaaaaga aaatgcggat gataacgtcc
      361 gcactatcct tatgtataag gctgtcagac gtcttaagca gaccaaggat atccgctttn
      421 nnnnnnnnnt gtggatcagg tgaagaaaaa cggatatttc cgtggaatga cagtttttgt
      481 tgacgctttc aacgatttct cttttgaaga acttaaaatg cttgacgctt gtatcgcaca
      541 gtgcaagaat ataacgttct ctctatgtat cgacaatgaa agcattagaa gatatgcaaa
      601 tcaccctttt gctgatactc ttaaaacgtt gcagcagata acagatattt ccgctgatca
      661 taattacaag gtcaacacgg tagagtgtca taacagctca tttagagtcc ctgaacttga
      721 atatgtgagc aaagagattt acaacacctg taaaaagcca tacgtcggaa agtgcgaaaa
      781 tgtttctgtt atatcagcaa cggatattta cgaagaaagt gaatttgtca gtggaaagat
      841 atgggagctt gtcagagaaa aaggctataa attcagtgac atagctttgc ttgcaagaaa
      901 ccttaaagat tgtgcttccg cttttgaggg tacgtttgac agatatgaga taccatattt
      961 ttcagactgc agtgacagtg tttcttcatc atctctggtg aggtatatga actcgctttt
     1021 caagtgcttg ctttcaagaa agtattccac ggacaatatt ttgaaataca taaaatcgcc
     1081 gctttttcca atgctgaata tcgagatatc aaatctggaa gactattgca tacgctggag
     1141 cgttgagggt gatatgtggc tcagtgattt tactgttgca ggtgatctca gcgaaaaaca
     1201 gcttgaaaaa ataaactgct caagacgaaa gataatcgag ccttttgaag catttaaaag
     1261 caaacttgtt aaagacgtta cagcagggga actgtgccgt gggctttatg atcttcttgg
     1321 cagcattgag ctttctcagc agaccttttc tgtagtaaaa aaagcaggaa cttctgataa
     1381 cgaaactcag cttgaaatgg caagaggatt gaaacaactt tggaactctg ttctttcggc
     1441 tatcaaatcc gtctatgact gcatgaatga cgaagttatc tccctcagac agttttatga
     1501 gcttttcaaa ctcatgcttt cgcaaatgag tgtttcacgt cctccgcaga aacttgactg
     1561 cgtaaggata tgcgacccga gccattcaag gcttgagggt gtaaagactg tctttcttgt
     1621 tgaggtgaac gacggcatat ttcctgcgac tttaagctct ggtggactta tcacagaaaa
     1681 tgaaaaaatg atattagagg gaagcgatat tcatatctca cacagcgcaa gaaaatcttt
     1741 tcagacagag aagcttgtgt gctacaacgc ttttaccctt gcagacgaaa agctctatgt
     1801 gctttattca gaatctgacc ttgtaggaga agctcatcac ccctctgcac ttgtttctga
     1861 gcttaacgga atgttcggca aaatcaatac caaagctcag gatatggggc ttgattttta
     1921 ctgcacttct ttcagaacag cttactataa atatatggaa cattctcgtg ataaaaacgc
     1981 tacaatttct gcgattaggt ctgtgttggg gttatcgcag gaatatgccg agaaacttat
     2041 gcgaacaaag aatcttcatg aaaacggtga ctataagctt gaagaaaaga ccgcagaaaa
     2101 gcttttcatg tcagaacagc ctgcaaaggt ctcgcctact cagcttgata catattataa
     2161 atgcccgttc aattatttct gcaagtatgg tcttggtctt aaaactgtca gaaaggcaga
     2221 tatcgatggg cttaacaggg gacttatcat tcatcagctt cttgaaaaga taatgcgtaa
     2281 agatgatgac ggcacaggct ttgacaatgc tttccttact ctatcagatg atgagataaa
     2341 agagtatgtc cacaaaaacg ttaccgaata tataaaggaa agccttggtg gaatgttcgg
     2401 aaaatctccg tcatttgtat ttttacagga gcgtatggaa agtaatatcc tgcatatcat
     2461 tcgttttatg cagaatgagc ttcttcactc aaaattcaga cctgctcttt tggaatacaa
     2521 aatagacaag tccgaaggag aagacagact tatcatagac gtaaacggca gagataagat
     2581 aatccttacg ggaattatcg acagagttga cgttttcgag gacgagaacg gtgaaaaata
     2641 tgtgaggata atcgactata aaacaggctc tattgacctt aaatattcaa tgctttacaa
     2701 tggccttaat ttgcagatgt tcgtgtatct taccgcttta cttgaaacca aaaatccagt
     2761 gaatatagac ggtggattga agcaggctgg tatcgtatac tacacgctag gaaacaagcc
     2821 taaaacatac gagcagacag ccgacgctga cgtagaagaa gccgagagca gatcacggct
     2881 aaacgctttc aagcctatag gaaaagtcgt tgatataaag gctgtcactg aagctttcag
     2941 ttcagaagaa aattacgcct atgcgccttt taaatattct aaaaccaacg gcaattcagg
     3001 cactgttcca gacggctttt ttacaaagct cagagaattt gctataggca aagtggcgca
     3061 gtttggtgaa cagctttgtc agggaaacat tcaggcaaga cctgtggcgg aagcctgcaa
     3121 gcactgtgat tttcatgata tctgcggcag gataaaccaa gaggacgtta cagacgtgaa
     3181 agacagcgtt tatgaagaaa aacttatgga agaactaact gacagaaagg agaaaaaata
     3241 attggctgat acaaaatgga ccaaagagca ggaaagagcc attagtgacc gtggcaaggg
     3301 cattatagtt tcagcggcgg caggaagcgg aaaaaccaca gtgcttatag aacgaatgat
     3361 acaacttctt tctgatgaaa aaaataaaat acctgccgac agacttcttg cagtaacatt
     3421 cacaaaagaa gccgcttcaa gcatgaaaga aaagctcagc cgtgcttttg acgaacagct
     3481 tcgccgtgac cctgaaaaca agtggttgct tagtcagcaa aatcttatcc agcttgcgag
     3541 gatatctaca ataaattctt tctgtcttga ccttgtgaaa agcaatctac atgaatttga
     3601 ttttcaggga ggacttgata tacttgatga cagtgtacaa aatcttattt tgcaggaagc
     3661 tatcacgcag gcttatgaaa agctttgtga agaagattat gaaagctaca aactgctgaa
     3721 aaacgctttt gcggataaaa ctctcaatga aatcaccgac aatctttata aattcatgcg
     3781 ttcgctgccg tttcctgagc gttggatagc ggaggtacat aaaaacttta ctgatgataa
     3841 gatttttgac agctttataa aagataatat atgtgtagct caaacaaagc tgaaagaagc
     3901 attttcaatg tatgaggttt ttgaagaata tgcctgccgt aaagagatag aaggcatccc
     3961 ttttataaaa catatcaaaa tgaatgaaac tgccgacagt gacagagaac ttcttcatag
     4021 gttaagaaag cttcttgaaa gcggcgatat agatactann nnnnnnnnat tgctaagtca
     4081 gattggaaat tcagcaccat aaaaagattt cccacgaaca aggctttcaa tgcatcactt
     4141 gacgatgata ttcgcattaa gttcaaggac aattatgcct tctgtgcatc atacagaaac
     4201 aaatataaag atatcgtagc taaaattacc gatatgttca ataacacaaa actaatcatg
     4261 aaggaaaata tgcttgaatc tgacagaata tttactgttc tgtgcagggt atgcgaagat
     4321 atagacaaga tctcacaaga aatgaagctt gaacgaaatg gcgttacttt tt
//
LOCUS       DAWEMR010000202         4316 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7369_length_4316_cov_8.270124, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000202 DAWEMR010000000
VERSION     DAWEMR010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7369_length_4316_cov_8.270124"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2215..2224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1153 a         1019 c          833 g         1301 t
ORIGIN      
        1 ccacgccttc attcagccat atacacggat aataaagcac cttatagaag atatctccct
       61 tgccctcctg actgaatgaa tccctgaata ttgcaggaat aaaatacact agaagcataa
      121 gaagatacac tattgtaata ttgacccagc tccaattttc atatgcggca tggcgtttgc
      181 cgtcgtcata aaaaagtctt atgaaaaata taagcatgac cgcaaagcct ataaatgagt
      241 ctacattact atttgtcata aagcttttga aagcagatgt gcttgacgga aagaccgtaa
      301 ccagagccgg tatcagcaca atgcctgcaa aggctgaaaa cagcgtaaga acgatcatta
      361 tccctattct cgacagaatg tactttaaaa attttattgg ctccataact acctccgttt
      421 tattctttgg ctttataaag tctttatgct ttacatctca tcttacacga taaagttttc
      481 agactttaca tctttcaatt atctgacata gttttatcaa agagtttatc cacaagctct
      541 cttagatata tattttctgt acgctcaagt ttagggtctg cgtttctgat aagctgggcg
      601 gcttcctgtg cttttcttag tatatccata tcctgtgaca tatccgctat cttcaatgca
      661 ggaaggccat gctgtctgct tccgaaaaag tcaccgggac ctctgagttt aagatcttcc
      721 tcagatatct taaagccgtc gttagtcttg cccaaaattt taagcctttt cacgctttcc
      781 tcattcacgt tgtctgtaat gaggatacaa cttgatttat atttacctct tcccacacga
      841 ccacgaagct ggtgaagctg ggaaagacca aatctgtcag cattttctat taccattatt
      901 gccgcattag gcacgtcaac tcctacctcc acaactgttg tggaaacgag caggtctatt
      961 ttatgctcct tgaagtcgtt catgacctgt tctttctgag caccgctcat tctgccgtga
     1021 agaagtccca cagtgtaatc tttaaacgtg ccctgagaaa gctttttagc ataggtctta
     1081 acgtcctgca agtccatatc actttcctct atcattggac agactatata accctgtctg
     1141 ccctcgtcaa gatatttttt cacatagtta aaagcacgct cgcgaagctt tcctgtaacg
     1201 gcataagttt caacgggctg tctgccctta ggaagctctt tcagtacaga aatatcaagg
     1261 tcgccgtata tcataagggc aagagttcta gggataggcg tggctgacat aacaagctta
     1321 tggggatttt caccctttga agcaagtgcc gcacgctgtt caacgccaaa gcggtgctgc
     1381 tcgtctgtta tgacaagggc aagctttttg aacttcgttg tctgctgaac aagagcgtgt
     1441 gttcccactg caacggcgta ttcaccgttt tcaagcccct ctttcacaag ctttttctgt
     1501 tttgcggtca tagagcctgt gagcagtgcc acctttacgc ctagcggttc aaggaagcct
     1561 ttgagtgtct cgtaatgctg ctcggcaagt atctcagtag gcgccataag tgcgctctga
     1621 cagccatttt catatacaaa atatgccgct cctgccgcaa ctgcggtctt acccgagcca
     1681 acatcgccct gaacgagtct gttcataggc tgttctttct gcatatctct gatacaatcc
     1741 tctatggcgg tttgctggct gtttgtaagg ataaaaggca gagcgttata aaagctgtcc
     1801 atatcctgca ttttcagcac aaagccgctt ttttcacggc ttcggctttt cataagctgc
     1861 ataccaagct gcaaaacaag aagctcgtca aagacaaggc gatttttcgc aagctcgcag
     1921 atgtgcatgt cttttggaaa atgtatattc tccaaagcat attcaagaga acagaaatta
     1981 tacctttgca ttatgctttt tggcagcggc tcatagatat tcttattaaa aacattcagg
     2041 gcgagcctta ctgtttttct gagcatttgc tgactaagac cctctgtgag gtgatagaca
     2101 ggctgtactt tatcttccgt atcggcagaa agtatcacag gagaatttat ctcttttcgc
     2161 acaaggtttc ctgttatcct gccgcagagg taatactctt tatccacttc aagcnnnnnn
     2221 nnnnaggtcg gtgacgtcgt cggtaaaaat gactttgtac actgacagac cctttctgat
     2281 acgggcttca gggagctttt taactactct gccctttact atgcaaggct cgttaattgg
     2341 tgcgtcggct atggggacag gcgaggtata gtcaatataa cttcttggat aatgacaaag
     2401 caggtcatat actgtcttta cgcctaattt ctcataacat tcggcacgct tttccccaac
     2461 gccttttaga tactctattg gtttctcaag atcttttaac aaaccctcac cacctgtcag
     2521 catttactcc acttaatacg attatatcat acatcacata aaaggtcaat gacttttatt
     2581 caaatcatac atatttacgc cattttccct gcacgttttg catttttgca caaaaaaaag
     2641 aacagatgaa aaaaatcatc tgtccttgat aaaaactata tgttattata ttttctcgcc
     2701 aaactcagaa tactcgatag aagctctgat gaaatctctg aacagagggt gcgctctgtt
     2761 tggtcttgac ttgaactctg ggtggaactg aacaccaacg aaccatgggt gtacattctt
     2821 tggaagctct actatctcaa caagcttttc gtcaggtgaa aggcctgcaa gcataagacc
     2881 cttatcttca agaagctttc tgtattcatt attaaactcc caacggtgac gatgacgctc
     2941 acggataaga ttctgtggtg ctgaatagat ctcagcacat cttgaattgt ctgcaagctt
     3001 gcatggataa aggccaagtc gcatcgtgcc gcccttttct gtgatatcct tctgtgaatc
     3061 catgatatct ataacaggat atggagtgtc accaaattcg gaagaatttg ccccttccag
     3121 tcctgcaacg tgtcttgcga actcaacaac agacatctgc ataccgaggc agataccgaa
     3181 cattggtatc ttatgttctc ttgcatattt gatagcttcg atcatgccct cgattcctct
     3241 gtcaccaaaa ccgcccggta cgattatgcc gtcgcagccg tggagcattt cagctacagt
     3301 gttctcattg agattttcag actgtatcca cttgatatca acttctgcgc tgttctcaaa
     3361 accgccgtga cggagagctt ctgctacgga aagataagcg tcctcaagct caacatactt
     3421 gccaacaaga ccgatagtta ctttcttgtt acagctatga actctgccta tcatttcctg
     3481 ccactcttta aggtctggct gtctgcactc aagcttaaga tgatgacaaa caacatctgc
     3541 aaggccctct ttttcaagcc agagaggcac ttcataaagt gatggcgctg taagattctg
     3601 tataacgtcc tctgctctta cgttgcagaa aagacctatc ttctgcttca tatcctcagg
     3661 tatctccatt tcagaacgaa gaacaaggat attaggctgg ataccgatag aaagcagttc
     3721 ctttacggaa tgctgtgtag gctttgattt aagctccttt gagcctgaga tataaggcag
     3781 caggcacaca tggataaaca cagaattttc tcttccaagc tcgatagaag cctgtctgat
     3841 agcctcgagg aatggtgtgg actcgatatc gccaactgtt ccgccgatct ctgtgataac
     3901 tatatctgtg tcagttgatt tgcctactct gtaaagacga tcctttatct cgttggtgat
     3961 atgaggaacg acctgaactg tgccgccaag ataatctcct cttctctcct tattaagaac
     4021 tgtccagtat atcttacctg tggtgacatt tgaattgata gaaagatttt cgtcgataaa
     4081 tctctcatag tgaccaagat caaggtcagt ttccgcacca tcgtcagtta cgaaaacctc
     4141 accatgctga taaggtgaca ttgtgcctgg atcgacattt atatacgggt caaatttctg
     4201 aatggtaacg ctgtagcccc ttgcttttaa caatcttccc agtgacgcag cggttatgcc
     4261 ctttccaaga cctgatacta caccgcctgt tacaaagaca tattttgtag gcatat
//
LOCUS       DAWEMR010000203         4312 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7374_length_4312_cov_9.264271, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000203 DAWEMR010000000
VERSION     DAWEMR010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7374_length_4312_cov_9.264271"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1065 a         1001 c         1035 g         1211 t
ORIGIN      
        1 tgatttattt ccagctggtt gcaatatagt tttctattgc agtggtttca gctggaagtg
       61 ttttctttgc gggcttatga taaaacaaag caagtgtgcc atcaagctca cttgcttttc
      121 tttttatgat tttccgggtt tcagcgtatt caaaaaatcc gccagcgcca tctgttcctt
      181 gtcattaaac cgcgtcaaag cactcataag ataaggattg atctcgctat catcttcacg
      241 gaagaattcc tccagggtga cgccgagtgc gtcgcagaag tacgacagcg tttcaacagt
      301 aggattacgc agtccaagct ctatttcacg caggtggctc tgagaaattc ccgccatatt
      361 cgcaagcttg ttcgtggtaa gccccttttg ttctctcaga tagattattc tggacgcaac
      421 attcatgcca ttcacctctc cttgtagtat aattataaag tatatagtga taatttatta
      481 cttttatata gttgacaagt tacatttatt gtgatataat tttaactgca tagagataaa
      541 tatcgaaatt tgcagatggt gaggatggaa aacatgaaga aaaagaaaga acttttgata
      601 actttagcag gagcagctgt attaggtggg attatcggag caatgtccgg atgcagcaat
      661 tcatatcggg taagaacaga tcccgaaatc atagggagtt tttatgaaat gggcggaaac
      721 ggcagctgcc ttgcatgctt aggctgcgtc agcaaatcgt ctgcggctga ctgctgcgga
      781 aatgttgagt actcatacaa cggttttaca gattgcttcg gactgacggt agatgacgac
      841 acaacggcag atgatattta tatgtcatac tacatatgca acggaatgta ctgcgttaat
      901 atgccggttg aatcgggtga cagcgtagtt cccgtgtacg gctgctacac agctcactga
      961 tatgcttccg ataattcata tcgtactgcc gatgtatgca gtctgctgcg ctcttggtgt
     1021 tatcgcagca gcaatattgc tgataagccg tgtcaagaaa tatggagtac ccccgattca
     1081 tgccatacag gtttgtattt ttgctgctat cggaacggtt attgggagta agctgttgtt
     1141 tttgctgact cagcttgata cgataatccc tgagttttct tttggactgc tgataggacg
     1201 tttcataaac agcggatttg tattttacgg cggattgttt ggtgcacttg cgggtgtaaa
     1261 gatctattcc gctgtaagaa aatatgacag tctgatgctg tttaatatgc tcgtcccgtg
     1321 tttcctgatg ttccacgcat ttgggagagt ggggtgcttt atgtcggggt gttgctacgg
     1381 cattgagtgt ccgtttggat ttgaaatgct cataagtccc ggtgtaacac gttttccggt
     1441 tcagcttgca gagagtgtct gcgatatcct tatcctcgtt gcagtgctga ttatcgaaaa
     1501 taaaaaggga acgcagaccg atattttgcg tatttacatg gtgtcgtatg ccgtgtgccg
     1561 attcctgctg gaatttttgc gtggagatga gatacgagga cattttctgt gcttttctac
     1621 gtcacagtgg atagtgctag gagtaattgt tttctatctt gtaagagcta taatgcactc
     1681 cacaagaacg aaacaagagg tgagcgacca atgatagaat atgtcatgcg gataatccag
     1741 aatgtcttta acaatgtaaa tacggcggct gaaaccccac tcggcgaaac tgttgtttcc
     1801 atcatcgtca taattgtagt aggcattata gtctgcataa tagcaagcgg aatcggaatg
     1861 atcgttgcgt cgatttttaa gggaatccgg ataaatcttg cgggaatgct cgcagcggtt
     1921 atcgcagaat ttgtcctgct gggttccgtg ccggaaaagg ggctgatcga ataccttatt
     1981 ggcggcttga taatacttgc cgggcttgtc ggctgtggaa tgacgctgat tataggcaag
     2041 gaatacggct ggggaaaatg ctcaaagaag cagacgggcg gctatgcgta ttactatgcc
     2101 gcattcatgc tgagcctgtt tgcggtatct ccgacgtggt acggaatgct tccggggaac
     2161 gtcaataaat ggtgtatata tgtgccgctc tttttgtccg gaatggttgc ctgtggtgag
     2221 cttttcggct cgttcagcga ggaggaacag caatgacgtt ttatgaactt ctgaatgtga
     2281 gcagtgactc ttcctcggag gaaattgagc ttgccttttc ggtacgcctt gcagaattca
     2341 gccgtgaagc cgcttcgggg tcagaggaag ccgctaaaaa aatctccgac ctgaaatacg
     2401 ccaggaaaat gctcacggat cccaaactgc gagcggaata cgacaaagcg ctgacggcgg
     2461 acgaaaatct gaatgcttgt ctactgcgta tctgcaatag cgtcgggcat tcgctgtatt
     2521 cagcctggtg ttgcccgagt aaggcgaaca gatatacacc acaggcttga agtgcattgc
     2581 cttttccgcc tttatagcgg ctgtaagccc tgcgtatgcg gtagggtcgg gatagcgttc
     2641 ggagttatag tagcttacat cactcattaa gtcacacctc ccgcaactgc tgtcagcagt
     2701 ccgtttacga agtttccgta gtcctcccgc ccgagttcct gtatatcact gatgttataa
     2761 tcgctgtagg ttgagcgaac ctgtcttgtg aaaccgagtt tgcacaagga taaagtcaga
     2821 gccacgacat cattaagcgt cgggtggtgt cggctgaact gctcccttgt acattcgggg
     2881 catattacct ttgccgacag cgggtggtct ttcttggctc tgaacagttc gttcagagga
     2941 acgggtactt cctttccgca tttgcagcag acagaaaagg tgtttccttc aagaacggga
     3001 ttttccctgt tgttgttttt tgtgtaaatc atttttctcc tccaaaaaaa ttttgattcg
     3061 gtttattgcc ttcacctact aacggacagg caaaagcaaa aaccgaacca gcattcaatc
     3121 ttttttataa aaaacacatt catacccgtc agctcgcaac ggaagtcccg ctgaccacgg
     3181 cggagttcta cccatcatct cgcagatttc agccacgtta gtgtcctcgg gacactcgat
     3241 aataagttca tcgtgaacgt gaccgcagat acggtaattc cacagcgtcc gcatagcgta
     3301 gcagagaata tctctgctta tcgcctggac gatattctcc acgaacttag gaccgtagct
     3361 ttcaatacgc tcccacttct ttgttgcacc tactccctca taagtgacag actcgccgcc
     3421 gaacttattc tcaccgatac ggggtttgac gtatgaaagc cgtctgccgc tcggcaacgt
     3481 gatgaacagc attccgctct gatattcaaa ctgaatgcca tgtgtgtctg cgcgaagcct
     3541 ttgtcgtatt gtatccttca cgcagcggtc gacttcccac cagaaccgaa ctatattcgg
     3601 gttggaactg cgccacatat ccaccagcgg ctgtaactca tcctccgaca accccatctc
     3661 caatgcgccc atagctttca gagcgccgac cgaaccgcca taaccgagcg ccaactctgc
     3721 gattttgcct ttctgtcgca gatgaccgtt cacaccatgc ttttcaacag gaacacggaa
     3781 catctgactt gcggacgcac aatagatatc tccgccactt ttgaaaacat caagccgcca
     3841 attctcacaa gcaaaccagg acagaactct tgcttcaata gccgaaaagt cagacaccac
     3901 aaatttcatt cccgatttcg gaacaaatgc cgtgcggata agttgggaaa gcgtgtccgg
     3961 aatatcgtca tacagcagtt ccatggcttc gtagttgccg ctttccacaa gctcccgagc
     4021 ctgttcaagg tcggggatat ggttctgcgg gaggttttgc aactgtatga gccgccccgc
     4081 ccaccgacca ctgcggtttg cgccgtaaaa ctgaaacatt ccgtgtgctc gaccgtcagc
     4141 gcagacagca ttcttcattg cctgatattt cttgaccgag gattttgcaa gctgctgacg
     4201 gagttccaga acctccgcaa gctgcggcgg tacggttttc agcattgacg ctacctcctt
     4261 tttgccaagg ctgtcaacct ccaagccgtt ctcggagagc cattgtttca tc
//
LOCUS       DAWEMR010000204         4277 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7437_length_4277_cov_9.935576, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000204 DAWEMR010000000
VERSION     DAWEMR010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7437_length_4277_cov_9.935576"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1393 a          756 c          978 g         1150 t
ORIGIN      
        1 ataaaaaagt cgttaaaaag tttacgaaaa ttcacaaaat gcctttgaca actcgaggca
       61 aatgtggtat aatgtttaaa tgactgtagg gtgcagtgcg tctaaatgcg acattataga
      121 gaaggtgaga cattttggga agaaaacgca ttggagtgat attgtctgag gcagaaagct
      181 attatcagga aagattgctg agaggcgtaa tgacaaaggc ttttcagctt gactatgatg
      241 tgcttatctt cacaagcttt gtcaaagagt cattcttaaa agcagtttct gttggagaaa
      301 tgcagatata tgacgttata aattatgata agctcgatgg cgttatcgtt ttggcagata
      361 cattaaaaat gcctgggctt tttgacaaaa tatgcaggga tatagatgag aaatgtcatg
      421 ttcctgtggt ttttatagat ggaagctata aggactatga aagtatttat acccgtgacg
      481 aaaaaccctt tatgcaattg acagaacatc ttatcgaggt ccatggatgc aggaagatac
      541 tttttttgag cggtccgctg gagactgcca ccactcgtga acggcttgcg ggatataagc
      601 gtgcccttga aaagcatggc atagagtatg atccggactt tgtctgcttt gacggaggct
      661 tctggtatga taaagcggca gaggtggcaa ataatataat ctatggcaga cgtaaaaagc
      721 ctgacgctat cgtttgctgt ggagattata tggctatagg cgttgtacat gaatatcaga
      781 aaaacggttt gagcgtgcct gaggatatga tagttgcagg ttatgacgca atagatgagg
      841 ctatccattg cgtgccagct ataacctcgt gctcaccgcc gatctttgaa actggcgtga
      901 acgcagtaat gaccattgag gcaaggatca aaggcgttga accgcagggc attatagagg
      961 acggcggcaa ggtggagata ggtgcttcct gcggctgtca tgaggattat agctatacaa
     1021 agcgtgatct tttatcatct cagaacaaga tgaattatca tgactttttg gacagcaata
     1081 tgaccgatat aatggcttgc tctaaagatc ccgatgatct gatggccagg atacaatatt
     1141 ttctctatct tatcattggt catgagaggt attatctctg ccttaatgag gattgccttg
     1201 gagagcatga gatagtcggt gagatctcca ctgttccaaa ggaatataca gaaaatatgg
     1261 tgctttatat aagaaacgtt gacggcaaaa gcagaactct tcatgatcct tttgaactca
     1321 aagagatatt tcctgatctg gatgaagaac gagaaagccc ttgtgcattc tttatcaccc
     1381 cggtacattt tatagatcgg tgctatggct atgctgtatt gtcttacggt gacgaaataa
     1441 aatcgataga tatcacttat cgtaattgga caaatcatgt aagcaatgct ctggaatcaa
     1501 tgcggatacg aaactccata gctaatcttg cagtccgtga cgctatgaca ggtgtttatt
     1561 cgagaatagg cattgagaaa aatatccagt ttgttattga cagaatgtca aatccgaaaa
     1621 ataaggcttt tatcgctgtc tgcgatctgg actgtcttaa aaagataaat gataattttg
     1681 gccataactg cggtgatatt gcaataaaag cgatcgcaga cgcagttttg gcatcgacaa
     1741 aaaatcatga gatatgcgca aggataggcg gcgatgagtt tgtggtgata ggctgtaacg
     1801 actataacga caactatcct gaacgatttt ctgcaagggt caatgaccgt cttagggtat
     1861 ataacaagtt tgcaggtaac gagtttgagg tcagcgttag tataggcggt atttgtaaac
     1921 atataacaga tgtccacgag cttgaagata tgtttacaca ggcggataag ataatgtatg
     1981 caagtaaatt ggaaagacat aaaaacagga aatagcaata gataaaatat cagtgtatta
     2041 caacggacgg tcagtgatcg tccgtttttg cgtgcgagag acacatatcc acttgactta
     2101 taggctcaaa tgtagtataa tgtaaaagtt aaggcggttt tctgcctgtt agtaagacga
     2161 ttttttcgga aaggaaatgg ataataaaat ggctaaagct aaaaaaataa cggctgctct
     2221 tactgccgca ttgctttgta ctgcgagcct taccgcttgc tcggacacaa gctatgtgat
     2281 gacagcaggt gacagcaaaa taaacgcagg tatctacatt tataatgagc ttacagaaat
     2341 gtcatatcaa atgacgatga tgtattatca gaaaggcatc aaaaaggact atttcgatca
     2401 gaaagtagac ggaaaggcat ttgacgagta tctttcagat tatgctctca ctgcaactaa
     2461 ggagtatgcg gctgtagttg acaagttcaa cgagcttgga cttactcttt cagatgagga
     2521 tataaagtcc attaatgaca gcataagcag cacatgggat tctcagggcg agttttacga
     2581 gtcagagggt atctcaaagg aatctgtaaa gcttgcgctc aaaggctcga aaatgaggga
     2641 agagcttttt gatcactact atgcagaggg cggcgaggaa gccgtttcag acgatgaaat
     2701 ggtgaagtat cttgatgata actacctgag atataagtcc atttcctttg caaaaactaa
     2761 agcctcaact gattcttcat catcagccac cgattcttct acagactcag cagacgctgc
     2821 aaatgaggaa gccaaggcaa aaagagatga gttccttgaa aaggctcagg gagtttcgtt
     2881 tgacgatttt gatagcatca tagacgaata caatgattat gttgcatcaa agaaagcctc
     2941 agattcaagc tccgccgctg acagcgattc atcagccgca gacagcagca cgacttctga
     3001 cgacacttct tctgtagcag agagcgacac aagttccaca gcttccgacg atacatcttc
     3061 tgtatctgac agcacagcag attcaagcac agcagcgtca gacacagatt cttccacaga
     3121 cagttcttct tccgcacctg acccgtatgc aaatgaaaag atgatgaatt acggcacaat
     3181 ggacgattca cagaaagaca ctacaaacgg caagatactc aaagaagtaa gcggtatgag
     3241 cacagacgtt gcaacagctt atgaggacga taacgcatat tacattctta tcaagggcga
     3301 tgttaaggac agagatacag aatatgccaa ggacaatcat gaagatcttc taaaagaaat
     3361 gaagagcgat gaatttcaga agaaattaac aagctgggta gaaaagcttg acatcaaggt
     3421 gaacaacaag gctataaagc gttatacgcc gaaagtggtc tacgataagc agacagagta
     3481 ttacagcaag aacaataatt catagcagat aaaattaccg ccgcaaccga gtttggctgc
     3541 ggcggttttg ttatgttgac aaatttgaga aatacgatat tgttgaccct atccggcaat
     3601 agtggtaatt ggtaggcttt aaggagcatt taaagcccga aggatcaagg attttcgtgg
     3661 ttgaaacgat aaaaaacctg aaaacttggg aaatccaaaa acgctgatag aaagcctgtt
     3721 gacaacaaat aaatagtctt cattagcgga cagtgtttca taaatttttg aagtgctgtc
     3781 cactattttc gtgtctaaaa ttgaatactt aataaagcac tttttggtgg ctgtcatctg
     3841 acggtcacca agaagtgctt tttcttttct ataaggattg ttttatcatc ataatcatgt
     3901 ttaagaacac ttgtgagtga atcggtaaga aaagatatac ttgattcatg gaaaacaagc
     3961 ctgactactt atttaccgtt tttaagaata atgatctcgt taccctgctg aacagcttgc
     4021 agataatgac caaaattgtt ctgaacatcg gttactgttg cagtgatcat aataacacct
     4081 ccaaacattt tcgctaatat tagtttgtga cattttcgct aaaatgttaa tggcgaactt
     4141 tagaaagagg aggaaaaatc aattttaata caaaaagaaa atatcccccg aaaagtgttg
     4201 gcacggcttg acaaggggag caaacgttca aagcaatatc agatagactt tcatcgtggg
     4261 caaaacagcc aggggtg
//
LOCUS       DAWEMR010000205         4272 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7448_length_4272_cov_8.318947, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000205 DAWEMR010000000
VERSION     DAWEMR010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7448_length_4272_cov_8.318947"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3449..3458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1346 a          820 c          903 g         1193 t
ORIGIN      
        1 tccaatctat aaccattttt cttctttttg ttatcgttca taagatcttc tccacaatat
       61 gattttaata ccattatact atatccctct gcctttttca agccaaaatg acaaaatcgc
      121 agatattttt ataccataca cactgccatg tgataaatgc acaaaagaca atttctttta
      181 ttgtttaatt tttgttttta ccccttgcaa aaaagccgca tatagtgtat aataagtatt
      241 atattgcttg acgttcacta ggcgacaagc ggccgtatta actaagatac aggtaatttg
      301 atttaacaga aatgaggctt ttatatgtgc ggttttgttg gatttacaaa taaaatcaat
      361 gacgcttcca tagttcttgg aaaaatgatg gatagaataa aacatagagg tcctgactct
      421 gacggaaaat atgttgacga gcagatcgca atgggtttcc gccgactttc gatcatcgac
      481 ctttcagatc aaggtagcca gcctatcttc aatgaagata agtcgttggt acttacattc
      541 aacggtgaga tatacaacta caaggatctc cgtgaggagc ttgtagcgtc tggtcacaag
      601 ttctatacac agaccgactc agaggtgctt atccatggct atgagcagtg gggcgaggat
      661 atgctcgaca agctgagagg aatgtttgca ttcgttatct tcaacaagaa cacaaacgag
      721 gttttcggtg caagagattt cttcggtatc aagcctcttt actacgcaaa aatgggcgaa
      781 actcttatgt ggggttctga gataaagagt tttcttgacc accctcattt taagaaggag
      841 cttaacacag acgttcttga aacttatctc acattccagt attctcctac aacagaaact
      901 ttcttcaaaa atgtttacaa gctccctgcc gctcactgct ttacatacaa gaacggcgag
      961 atgaacgtaa gaagatattg ggaagtcaag tttcatgcgg acaatggtcc ttctttagag
     1021 gattgggtga ataggatatc tgacactttc aagaactctg ttgaggtaca caagtttgct
     1081 gacgttgagg taggttcttt cctctcaagc ggtgttgact caagctatgt tgccgctgtt
     1141 gccaacgttg acaagacctt tactgtgggc ttcggagagg acgagaaata caacgagata
     1201 ggctatgcta aggagttttc aaagtatatc cacaaggaga atttctcaaa ggtcatcagc
     1261 cctgaggaat actggaacag cctttcaaaa atacagtatc acatggacga acctctcgca
     1321 gaccctgcgg ctgtggctct gttcttcgta tgtcaaatag cttctgaaaa ggtcaaggca
     1381 gtgctttcag gcgagggtgc agacgagata ttcggcggat acaacatcta tcacaaccct
     1441 gctgatatgg caagctactt caagatacca agacctatca gaaaggctgt gggtgctgtt
     1501 gctgataaac tgcctcacaa gcacggcata aactatctta taagaggttc taaggacctt
     1561 gacgagagat ttattggtaa cgcatacatc ttctctgaga aagagcgcaa ggacattctg
     1621 agcataaaaa caaacgctcc tgacgctatg gctatcacaa agcctttcta cgataaggta
     1681 agagatcagg atcaggtaac gcagatgcag tacattgacc tgcacctttg gatgacaggt
     1741 gatattcttc ttaaggcgga taaaatgagc atggctcact ccctagagct tcgtgtaccg
     1801 ttccttgaca gaaaggtaat ggagcttgca gagcagatac ctgtaaagga cagagttaca
     1861 gaaactgaaa cgaagtatgc aatgcgtctt gctgcacttc aggcttgccc tccacagaca
     1921 gctaagaaaa agaagctcgg cttccctgtt cctatcaggg tatggctgaa agaagataag
     1981 tattataata tagtaaagga taagtttact tctcctcagt cggcacagtt cttccacaca
     2041 gataggcttg tacagcttct tgacgatcac cgtgcaggca aatatgacta ttcaagaaag
     2101 atttggacag tgttctcatt ccttgtttgg tatgatgttt atttcagcga caacgtataa
     2161 ttacacaaat agtaaaagcg gttacctcca gcgggttacc gcttttactg taattttttt
     2221 tatatgacta tcttttagaa atactttttt ctttatgtag tagcatcaac atatatatat
     2281 tagggtacag ctaataaaat atgttgcggt caatgtaaaa tcattgaaga ttaaaggtaa
     2341 aaaagcaaaa atattgtgtc ttagctattg taaattttaa aataatatgt tataataaat
     2401 cttaaataaa tgagaggaac agggggtatc atatgacaat ggctacactc aaaatagttt
     2461 tctgggcttt agctttcgtg ttctttgttg tatgcgaggt cgcaacagga acggcacttg
     2521 tgtctatatg gcttgctata gcagcgctta ttgcgatgtt ttgtgccata gctaagcttt
     2581 ctttccttac acagtgcatc gtattcgtcg taagctcgat catacttctt ttgcttacaa
     2641 gacctgtggt caagaagctc cagaacaagc cagttgcaac aaattacgag cttgatgtgg
     2701 gaaaaactgc aatagttatt gagcctatag ataactctgt gaacaaaggc agggtcactc
     2761 ttgatggcac agattgggca gcaagaactg aggacggctc aaagatctca gaaggcacac
     2821 cagttaaggt cgtgcgtatt gacggtgcaa agctgatagt ttcagaagaa aagtaacagc
     2881 ttacataaac ttattttttt aggaggcata taatggaacc tggaatttat atcgtacttg
     2941 ctatcatcgt attgcttata atcattgtta tcagcaacat caagatagta ccgcaggcat
     3001 atgtatttgt agttgaaaga cttggagctt ttcatgcggc atggggaacg ggacttcacg
     3061 taaaagtacc ttttatcgat cgagttgcaa aaagagtatc tatcaaagag caggtagttg
     3121 acttcaagcc acagtcagtt atcacaaagg ataacgtaac aatgcagata gacactgttg
     3181 tgttcttcca gataacaaac gcaatgcagt tcacatacgg cgttgagaga cctatctcag
     3241 ctattgaaaa tctgacggca acaacactca gaaacattgt tggtgacctt gatcttgagg
     3301 ctacgctcac ttcccgtgac attatcaaca caaggatcac agcaattctt gatgaagcca
     3361 cagataggtg gggcatcaag gtgcagagag ttgagcttaa gaatattctt ccgccaagag
     3421 aaattcagga cgcaatggaa aagcagatnn nnnnnnnnga cgtgaaaagg tcattcaagc
     3481 agaggctgag aagaaatctc aggtgcttgt tgcagaaggt gagaaagaat ctaagatact
     3541 cagagcgcag gcagacaagg agtcacagat acttgcagcc gaagctgaga agcagtctat
     3601 gatacttcgt gcagacgctg tcaaggagca gaagatactt gaggcagaag gtgaagcgca
     3661 ggctatcgac atggtacaga aggcgattgc cgagagtatc gtcaagctga atgctgctaa
     3721 ccctaacgag caagtaatcc agctcaagag ccttgaagca tttgccaagg cagcagacgg
     3781 caaggctaca aagatcatta taccaagtga gatacagggt attgcaggtc ttgcaacagg
     3841 acttaaagaa gtcatgacag acgttaagaa gccacagtaa aacgcatata gttaatcccc
     3901 ttcaaggcaa atgctttgga ggggatattt tttttcattg aaaagcgaga aggacactga
     3961 gataaactca atgccctccc aatatcatta agctaaaaaa atcaagaaat aaaataacta
     4021 tgatacattc gcataaatcc gttaactatc ggctctaaaa atgctccgtc agcaagaaaa
     4081 attgtatgcg ttattatact tgatatactt atttttccat agccccaact aaaaatagat
     4141 atttaagttt tctattttcg ttttcattaa catcgggaat aatatttgta atattcacca
     4201 ttgttttatg ataaatatta atcatacggc atttattaaa tacaattaaa tatccacgtt
     4261 caagatctgg tt
//
LOCUS       DAWEMR010000206         4210 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7566_length_4210_cov_27.475812, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000206 DAWEMR010000000
VERSION     DAWEMR010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7566_length_4210_cov_27.475812"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1327 a          794 c         1057 g         1032 t
ORIGIN      
        1 tcaacaaggt caaggctgtg ggaatgtccg acgcacagct ttacaagcag gcaggaaatg
       61 cggtgacaac taatgttatc gaggctctgg caaagtatat tttagatatc gaaaaggaga
      121 atgcaaatgg ataatatggt aaaaacattt gtgaatgagg agtttggctc ggtgagaact
      181 atcgaggaaa acggaagggt gatgttttgt ggaaaagacg tggctctcgc attggggtat
      241 agcgatacga acaatgcgat aaaaactcat tgcaaaaaag atggggtggt gattcaccac
      301 ctcatagatt caatgggcag aaagcaaggt gctaagttta tttctgaaag taatctttac
      361 agacttatat cccactccag acttccctct gctgaaaaat ttgaaagctg gatatttgat
      421 gatgtactcc caactatacg cagaacaggc ggttatgttt caaacgaaga aatgtttatt
      481 gaaaactacc tgccgtttct tgacgagccg taccgtgacc tgttcagact tcagatgacg
      541 gctatcgaaa agctgaacga gcgtatcaga aatgacaagc ctctcgtgga tttcgctctg
      601 cacgttgccg acagcgagga tctcatagat atgaatgcta tggcaaagct tgctgctgat
      661 aaaaatttca atatcggcag aacaaggctg tttcgctggc tgaaagaaat gggagttctt
      721 atgtcaaaca atctgccgta tcagagatat attgaccgca gatattttgt cgtaaaagag
      781 tcggtgtttg aggtcgaggg tatgaaaaag acttatcggc agacatttgt tacgggaaag
      841 ggtcagcttt ttatcatagg tctgctgaga aaatattacg gaaaggaggt tttgcaataa
      901 tggatatcga tgatatttct ttgcatgagc tgagaaaaat gaatgactgc gaaggacttg
      961 tcctgcaagg gtgcggcggt gatctgcagg aatgggtgaa cggcataaat gatatgctga
     1021 cggaaagcgg aatattgcag agcggcagta agtttgaaaa agcattctct tttaaaaacg
     1081 gggaccttac ctgtctgctg tttccatttg atgacgttca gcttgatgtc ggaaagcttg
     1141 caatatggcg gctccgaacg agggaggatt tcggaagcac atggctgtcg gactatgtgg
     1201 agaactcgtt gggaggatat attcaggagc agtcggaaac cgaagatttt gaaatggaga
     1261 tgatgtaaat gacgtatttg ttcattggaa tgatgatagg tgttatcatc ggaacaacga
     1321 taggttctct tgccgtggct tttaagtctg ctgaaaaaga gatcgcacgt cttgataagg
     1381 aggtcaaaga tgcacacaaa cagagttaaa gccaagatag acttcaagtt ctgtatgggt
     1441 aatattcctg caatgctgag agcaacaaag cctgtgcttt cggaaaagca atacaaggag
     1501 ctgtgtaatg aggtaaacaa agccgatggc taccttgaac agaaacgcat tatattcagc
     1561 tacgtcgatc caatgatcaa gggctgaaga aaacacaatt aaataaaaac taatgtcgtt
     1621 ttgccaacag cagataaata aatctggtgc ttgcaaagcg actttttcgt ttgcatactt
     1681 cttttctgct gtttggcaag gaaaggagct taatgaacag ttttatgtct tgggtaggtg
     1741 ggaagaaaaa tcttcgtgac gaggttcttg caagatttcc gccttactat gaaaggtata
     1801 tagaggtctt cggcggtgca ggctgggtgc tgtttcacaa gccgcccggt gcagattttg
     1861 aggtgtacaa tgacttcaat tcaaatcttg caaatctgta ccgctgcgtc agagataagc
     1921 cgaacagact caaatacaaa ctgagatatg tgcttgattc aagagaagat tttgagtatt
     1981 tagccctact tcacaagcgt ggtattttgc ctagacttta tgacgttgac agggcggcta
     2041 agttttatca gcttatacgt tacagctatg cttcgggact tgacagcttt ggaagtcagc
     2101 cgcattcgat gtggtcggac ttccctatga tcgaccttgc cgcaaggcgg ttgcagaagg
     2161 tagtagttga gaataaagat tttgaaaagc ttatcaggca gtatgacaga cccgtcagct
     2221 ttttttactg cgatccgccc tactttgcta cagaaagcta ctacaaggac gtgggattta
     2281 cggcaaagga tcatatcaga ctgcgtgata cacttttaga gatcaaaggc aagttcttgg
     2341 tttcatacaa tgactgtccg gagataaggg agatatggga caagcctaat atccacattg
     2401 aggagataag cagacttaac aacttagcac agcgttatga cgcaggctgt cagtacggcg
     2461 aactgcttat ctcaaattat gacacaagcg aacgtgcgaa agctatcaaa cagctttcac
     2521 tttttgatta ggaggttttt atgaaaaaga taatattggc acaggtttta acggcaaaag
     2581 gcaaaacaga attttcaggg ctttatgaca ggcacggaaa tgctgtaacg gtggaggtcg
     2641 aacgtgacag cagcggcgtt tcacttatcg tatggcagga cgaacagaaa aagcagcact
     2701 ttgactgtgg gatatccgaa gaagagatac agcgtacctg tgcgatgtat gatgactgcg
     2761 gcgattgtcc gctgaaagag tattgtgaaa gtgaggcaaa agaatgagga tacttgtgtt
     2821 tgaacctctg aaagaaccgt atgtgaaaga tatcgaggac gatatccagg cgatgcagga
     2881 agtagtcgga gaaagcattg aacctatata ttttgagcag acaaacaacg ccctttgctg
     2941 gtgtaatgat gagttcctgc tgaacggctc aaagcctaac aggatagtag gaaacactct
     3001 tgttcacggc actttctata tttcgggcaa ctgtctgaac gaatacggcg aatgggactc
     3061 ttgttcactt accgatgaac agatagaaaa gtacaagcag cagtttgatc atatcatagt
     3121 agatctgccc ggtatcgggc tggttgctgt gagagaaaca aagccggaag tgatccaacc
     3181 gcttgaagaa tatgaagaag aaccggaaat cgaacagaca atgtaaagga gaatgaaact
     3241 atgagaatac acgcatctat caacagaatg gtaaacaaag aggattcagc aatcaaggca
     3301 tatgccagcg taacaatgga cggtatgttc gccgttcacg gactgagggt catcgagacc
     3361 gaaaagggca gatttgtcaa tatgccctcc acctcataca ccgataagga cggcaacaag
     3421 cagtacagcg acaccttcca cgccataacc aagtcggcga gaactgccgt aaatcaggct
     3481 gtgctcaatg cttatgatct caagctgcag caggttcagc agacggatat cgaggtggag
     3541 aacactcccg atgaggaaat gtcggagcct gaagatgaac ctgaaccgga gctaagtatg
     3601 tagtttgcta aagcatttgc tgtgtatctt gtaacaaagg tagaactttt gcaaaaagta
     3661 tagatttgtg gacggatata tgatatattg cttgacaagg agcgtgatga tatgaaaaga
     3721 gcgatatata tttcaatact ttgcacagta atgctgactg cctgcgggaa tgtatcggag
     3781 tcaacgaaaa gtgcagaaac ctcagatgtg agccaaactg cggtcacggt aacatcggca
     3841 aaggaagaaa tggatacaag taaaaccaca acgaccatta aaaagatttc gcagacggta
     3901 tcatcttcct caaagctgac agatactgaa gtaactgcgg ttaagaaatc aagcacttca
     3961 aatacaggcg gcaattcagt tcaggggcag aataacgctg tacagggtaa taaccaagct
     4021 gtgcagaata atactccatc caacaattct caggacaata acttagcgga gcagaacgct
     4081 cagccgtcca atgagaagtc tcagaataac gagccgtctg ccacccgatc aacaacggca
     4141 aagcagtcag catcggcaaa gcctgcggta acaaatccgc ctgacacaac gacaaagccg
     4201 caggctacaa
//
LOCUS       DAWEMR010000207         4190 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7602_length_4190_cov_10.187424, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000207 DAWEMR010000000
VERSION     DAWEMR010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7602_length_4190_cov_10.187424"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1245 a          814 c          996 g         1135 t
ORIGIN      
        1 ctctgttatc gcaggcgagc taccgaaaat attcagagag ggacttatct ttgacgcttc
       61 aaagctcggt ggctttttga atgtgactgc ttcagacctt agacttttcc ctgaccctga
      121 tacgctggcg cttcttccat ggcgaccgca gcacggcagg gtagtaagat ttttctgcca
      181 cctgaaatat cctgacggaa cggctttcga gggcgacggc aggtttttcc tcaaaactgc
      241 tatcgcatat gcaaagggta agggatataa cttccagata ggtacttcat gcgagtttta
      301 ctgctttgaa aaggacgata ctgatatgcc tacaaagcgt cctcacgatt atgcaggcta
      361 ttgtgacgct gcgcctgttg acaggggaga aaacctcaga cgtgacatat gcctgacact
      421 tgagcaaatg gatattttcc cagaaagctc aagacatgaa accggtccgg gtcagaacga
      481 gatagatttt cagtattcaa acgcactgaa agccgccgat aatcttgtga cgttcaagaa
      541 cgttgtaaaa tctgtggctg acagaaatgg gctttacgca acgttcatgc caaagcctat
      601 cgtcgacaac tgcggatctg gtctgcatat aatgctcatg tgcatgaaag gcacggaaaa
      661 tgttttcaaa cctcagcttg gcggactttc caaagaggct gagcgcatga tagcaggaat
      721 actgaaaaac gttggcgata tgacgctgtt tctaaacacc actacaaatt catacaaacg
      781 ttttggcagt ctgcttgcac ctaagtacat ttcatggtca catgaaaatt atgcacagct
      841 tcttcgtgca ccgcttgctg atacagatga gaatggtata atactccgtt cagcagacaa
      901 cacctgtaat ccttactttg ctatgggtct gcttatttat gcctgcatag acggagttat
      961 aaataaggaa gaacttccaa aaccttttaa tttcaatata ggctcggcag atgaaagcga
     1021 gcttgcaaag cttgaaacac ttccgcagtc tttgaaagag gcagtggaac gtgctgaaaa
     1081 gagcgagttt cttgcggata ggcttcctga gcatatgttg gagcgtttta tcgcaataga
     1141 aaaggagctt atcatagaat atgagagagc tgccgataag gagcagtttg aaacagccag
     1201 atatttttat catctgtaat gtcagagaaa gctaaaaaaa ggaggtgggg aaatggacag
     1261 gatacttgtg gcttcaagca atgacaaggc aacagctatg cttgtgagtc tgttaaaaga
     1321 aacctttccc acctgcaaga cttctatagc cgcaacgggt cttgaaacaa ggcgagcggt
     1381 agggtcaggc agttatgact gtgtcgtgat aaactgtcca ttgtcagacg aatatggcaa
     1441 cgagcttgcg gagatagtct gcacctcaac tgacgcaagc tgtgtggcta tcgcaaagtc
     1501 ggaaaatgcg gatatccttg cggataaact ggaagatttc ggcgtaatgg tgataccaaa
     1561 gccgctaggc agacagtatt tttaccgttc aatgaaattt gtcagtgccg caagaaaacg
     1621 tatgctcgga atacggtctg aaaatctgaa gcttcacaaa aagcttgaag agataagaac
     1681 gataaacaga gcaaagtgct cacttatgca gtatcttggc tttacagaac agcaggctca
     1741 taaatatctt gaaaagcagg ctatggataa gagatgcaca aagatcgagg tggctcagaa
     1801 ggtcatcgag atgtacgagg tttaaatgtt acataattgt taaattaatg tcacgctatt
     1861 gcaattgacg gtgtaatgtt gtataataat aaaggaaaat ttaaaataac ggaggtcgtt
     1921 aaaaaatgtt agtttcagct aaggaaatga tgacaaaggc acttgcaggc aagtatgctg
     1981 taggtcagtt caatattaac aaccttgagt ggacaaaggc tatccttctc actgctcagg
     2041 aactgcagtc acctgtaatt cttggtgtgt ctgagggcgc aggcaagtac atgacaggct
     2101 ttaagacagt tgctgctatg gtaaaggcaa tggacgaaga gctcggtatc acagtaccag
     2161 tagcacttca ccttgaccac ggaacatatg agggctgcta caagtgcgta aaggctggtt
     2221 tctcatctat catgttcgac ggttcacact atccatttga ggagaacctt gctaagtcaa
     2281 ctgaacttgt tcacgttgca gatcagcttg gtctttctat cgagtgcgaa gttggttcta
     2341 tcggcggcga agaggacggc gttgtaggta tgggcgagtg cgcagatcca gacgagtgca
     2401 agactattgc tgatctcggt gttacaatgc ttgcagctgg tatcggtaac attcacggta
     2461 agtatccagc aaactggcca ggtctttcat tcgagacact tgctgctatc aaggaaaagg
     2521 ttggcgatat gccactggta cttcacggcg gtacaggtat tcctgatgac atgatcaaga
     2581 aggcaatctc acttggcgtt gcaaagatca acgtaaacac agagtgccag ctttcattcc
     2641 aggaagctac aagaaagtac attgaagagg gcaaggatct tcagggcaag ggctttgacc
     2701 caagaaaact tctcaaccct ggcttcgagg ctatcaaggc taaggtcaag gagaagatgg
     2761 aactcttcgg ttctgttggc aaggcttaat ttcagctaaa ttaacgtaaa taaacgggct
     2821 gtcgtttcgg cggcagcctt tttatgttat tttattttcc aattaaatag agtgtaacct
     2881 agatcaacca ctcacaaata aaaacaagga gattttttta tggaaatttt cagtgcacgt
     2941 cgtttaacaa acatgggact tgttgcagcg atatacgttg cggcaacaat gctttgcagc
     3001 tcgcttgcat acggacaggt acagttccgc atatctgagg ttttgatgct actttgttat
     3061 ttcaacaagg attacatcat ttcgctttct ataggttgtt tgatagtcaa tctattcagt
     3121 acgctcggaa tggtggacgt agtatttgga acgcttgcta cagttgtggc agcggttctt
     3181 atctatctgc tgagaaacaa ggtaaatctt gtagttgcgt cgcttttccc ggtgatatca
     3241 aatgcggtga tagttggctt tgaactgaca tatgtcttta agacgccgct tcttgtgaac
     3301 atgggctggg tagccttagg cgaatttgta tgcgtaacga tagttggtgt aatacttatc
     3361 aaggcgctta tgaagaacaa gtatttcagc aagcttgtga ctttcggcat gagtgatcag
     3421 gctctaagtg agcagtttaa aaacgggtga tgattccgat ataaaaatca gcctgacttt
     3481 ttagtcaggc tttatttttt ggcgttttta tcgaaaaaac tgcgaaaaag tattgacaaa
     3541 gtacgtgtaa tatgatataa taagtacgtt gcattgagca acgttaaata tgcggatgtg
     3601 gcggaattgg cagacgcgct agcttcaggt gctagtgatc gcaaggtcat gtgggttcaa
     3661 gtcccgtcat ccgcaccata ttggtggaac aaaaaagatg caccagttga aaagcccgta
     3721 aatacgggct tttttcatgc tttttaaccg gaaattttaa ggtgaaaacc gtggatgctt
     3781 tttcacgatt ttagccgacc ggaaggattt gaacgctaca caacgaaata ttgtcaaaca
     3841 aatcggcagg ctttgagcaa atttcgctct taacttgccg attatttatg aaaaaacttt
     3901 cacaaacttt tcaagactgt tttgtctaat atcacgaaat gtgataaatg acaaaacggt
     3961 ctttttttat ttctcaagga aggacgtgat acccgtgtgg cagctatact attaaaatgt
     4021 aaaataaaac aaaggaggaa atcaatgaac aacaaactta taatcaacac aaatctgctt
     4081 cgcaaagaat cggaattcaa aacgtatact tgcgtggtgg aaaaagctgt gcctgttccg
     4141 accgaagaat ttgagaggct gaaacacact ccgatgtgca ataacgatct
//
LOCUS       DAWEMR010000208         4181 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7619_length_4181_cov_9.052109, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000208 DAWEMR010000000
VERSION     DAWEMR010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7619_length_4181_cov_9.052109"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3608..3617
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1087 a          886 c          974 g         1224 t
ORIGIN      
        1 gtaatgaact atccgttccg tgaggcgatc atcgcctaca tgaagggtga ggctgccacc
       61 acgttccggg accgcattat ggtgatcctg gaaaattatc cgaaatgcac ggtggatgtg
      121 ctgatgaact ttgtctccac ccacgacatc gagcgtgcca tcaaccgttt cggcggtcag
      181 ccctgcgacg acaagagcaa ggactggatg gcatccaacg aactgtctcc ggacgagtac
      241 aaccgtggca aggctctgct caagggcgct atggtactcc agttcttcct gcccggcgtc
      301 cccagcattt attatggtga cgaggctggc ttgcagggct ggaaagaccc gttcaaccgc
      361 cgctgctatc cgtggggcaa tgaggatacg gaactggtgg aatacacccg tcaaatggcg
      421 aagatccgcc gggagcatcc ggcttttgca gagggcgcta tggagttcct ggtactggag
      481 gaaaaggtgc tgggctttgc acgatacatt ctgcagaacg gttccagcgc tgttgtgctg
      541 atgaaccgca gcgatgagac tgccatggtc tctctccggg acaagtgctt tgagaagtat
      601 gcgttcagca cagtaatcag cggcatctgt gacggcgatg ctgtcattct gccggcacac
      661 agctatgcag ttgtcagcgc cgttcgctcc aagcaagcaa aaacgcagta aattctccgg
      721 gatttttctg cattcagaca aaaggcggca gagatgccgc ttttttgttg gctgttttta
      781 tttgcggatt gttctaaaat caaagatatc tattttattt tgcaaagata tcaaaagcaa
      841 aactttcttt tttttgaaag tgtcgaaaaa ggctttacaa gtgtccggaa atgtgctata
      901 ctatatgagt cgccaatcga ggtgtggctc agtttggtag agcactacgt tcgggacgta
      961 ggggccgcaa gttcgaatct tgtcacctcg accaattgca gaaggctgaa ttacgaaaaa
     1021 agctcgtaat tcagcctttt ttcgttacct ggggtgtttt gcgaaaaaca ggttttttcc
     1081 caaaatggca agtaaatacg ggcaaatggc aagtaaaatg gcaagtggaa aacggtcagt
     1141 ttgtgccagt tgttggaact tttggagttg cctagcgttg tccaatcaag aaagaaggca
     1201 aatggatttg aacaaaaata tgaaaagcat acagcctata gaccccgtca ttacacttcc
     1261 agcctatctg aatcaaaggc aataccgtgc aaagtcacca gacgctcttt gtgcggtatt
     1321 gccttaaaat aaaataggca aaggatttgt cactgccaat cgcagcaagt cctttgcttt
     1381 ttattatttc cgttccgacc gcctgtgatt tctgtactcc ttaggcgtca ttccatagag
     1441 tgccttaaat ttgcggcaaa aatatcccac attttcaaag cctgctgtct gagctataac
     1501 gaaaacgctg ttttcagttt ccgtaagaaa cttggcagca cgcttcaaac gatattccag
     1561 agcataatta atgggcgatg tgtgcagata gcttttaaaa atattcagca cactgctttt
     1621 gcttatagaa acagatcttg caatgtcttc caaggttatc cgacgataat agtttttgtg
     1681 gatatgctgc atcattatct gcagctgtgc ttgtgcctgt gcggaggttt tgtttatgtt
     1741 ttgagtgtcg ttccaactta tgctttcgta aatctgcccc catagcttca taataagttc
     1801 ggttgttttc agttcggata tgcttttcga ttcctgtatg gcaaacacat ttcgtattgt
     1861 atcaagcatc tcacattgct tttcatctgt cggcgatatt atcaggcatt caaccgagga
     1921 catgagcaca ggctttatat atttgcggta tatcaaactg ttttctgcgg ctatcagaag
     1981 cggagaaaag accatattgg gaataattgt actttcgctg ctctcaaacc gatgtatcac
     2041 ctgtgtgttg atgaaaattg cttcccctgt gttaagcaca tatctgctgc tgcccacaaa
     2101 aaaatctgcc gtcccatttt ccacaagcac aaactcaacc tcggggtgcc agtgccagtc
     2161 aatacagtgc aggtcaaaga gccatatatc ctcataataa tatcgaaagg gatattcctc
     2221 actgccgtga cttatggttt cacgaagagt ttcgtcagtg atgatctttg cgtagccgtc
     2281 gtttaggttt ttcaaattta tcctccattt ttgcgatata gtgtaataaa aatgcgatat
     2341 tgtacattga aatatgccgt ttgttatgat atgatacaat tatatcaaaa agcgaaagga
     2401 acgtcaagat gaattacaga aaaactctta ttgcctgtta tcttggattt gttacacaag
     2461 ctattacagc aaattttgca ccgctgctat ttctcacatt ccacaatgtt tacaatatcc
     2521 ctctcggaaa aattgcgctc atatcatccg ttttctttat cacccagctt attgtggata
     2581 ttctctgtgc aaagtttgcg gacagaatag gctaccgcag atgcgttgtg ggttctcagc
     2641 tgttttcggc agcaggactt ctgggacttg cgtttcttcc tcagataatg cccgacccgt
     2701 ttatgggaat aatcatcagc acaattgtat atgcggtggg cagcggtctt acggaggtgc
     2761 ttgtcagccc tatcgttgaa tcctgtccct ttgagcataa agaggcggca atgagtttgc
     2821 tccattcctt ttactgttgg ggctcggtag gagttatcct gctttccaca gtatttttct
     2881 cggtgttcgg tataggcaat tggaaatggc ttgcttgtat ctgggcaata attccgctgt
     2941 ataatatgtt taatttcatc acctgtccca ttgaacaccc tgccgaggac gggcaaggta
     3001 tgagtatcgg aaatctttta aaaacccccg tgttcattat ttccatactg ctgatgatat
     3061 gtgcgggagc gtcggagctt tcaatggcac agtgggcatc tgcatttgcg gaatctgctt
     3121 tggatctttc aaaggctatg ggagatattg ccgggccttg cctgtttgcg gtgactatgg
     3181 gaataagtcg ctcactgtat ggaaaatacg gcgacaggct tgacctaata aaatttatga
     3241 taggctcagg catgctttgc ctgatatgct atcttgttgc ttccctgtcg gatattccca
     3301 tgcttgggct tgcaggctgt ataatatgcg gcttttccgt tggaattatg tggcctggaa
     3361 ccataagcat ctgttcgggg aaaatgccct caggcggcac agcaatgttt gcccttcttg
     3421 ccatggcagg cgaccttggc ggtgcaatag gtccgggtat cgtaggaaac atttcacaaa
     3481 gtgcaggaga tgatattcgc aagggcgtgc ttgcaggctg tatttttcca cttgtgcttg
     3541 ttattctgac ttttgtgatt tccaaaaatg tcaataaaaa caggcaggaa tgaaagagta
     3601 tgactatnnn nnnnnnnact ataaggcaat accgtacaaa gagcgtctga cgactttgtg
     3661 cggtattgcc ctgaatcagt acaatgtgat tcatatcgat atccagcagt ttgtcagcaa
     3721 atatcctgtg ataaaagaca tggtggcacg cttaacaaag gaattgttgt ttgatgtgaa
     3781 aatggaaatg ccggatgtta cattgtttga tgaaacagac ttggtaagga catcaaaaga
     3841 tattttttcg cagaaacggg aaaagtttat ctttatcatt gacgaatggg attccatttt
     3901 ccgggtacat cgggataatg caactgctcc aaaggaatat ctggattttc tgcgggattt
     3961 tctgaaaggg cagccctatg tggcacttgc ctatatgaca gacattctcc caatcaagaa
     4021 atacggtcaa cattctgcac tgaatatgtt caaggaatac gctatgacca atcaatatgc
     4081 actcgcaaaa ttcactggtt ttacagagag ggaagttcgt cagctctgtg aacgttatca
     4141 tatgtctttt gaacaaacga aagattggta cgatggctat a
//
LOCUS       DAWEMR010000209         4169 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7641_length_4169_cov_13.011181, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000209 DAWEMR010000000
VERSION     DAWEMR010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7641_length_4169_cov_13.011181"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1109 a         1098 c          760 g         1202 t
ORIGIN      
        1 ggcaatatct acacatacga ttattatata ctataacccc ttttatgtca atagcacaac
       61 ttacaaacag caccgcaaag ctgcaaggat tttcagcgat tttaacacga aaatccccga
      121 aaaatcgtcg tttaaagctt aaaagggact gtcgccctgc aagcgtcagt cccccatatc
      181 tgcatgatta ttttatgagc ttttccgtcc aattatgcgc acacatacat ctccatacaa
      241 aaaaaataaa gggtcaaaac aaattgaccc tttaaatacc tattaaagcg attttagacg
      301 gattcgcaac ccctattaaa agaataaatc tttctagttc tattatacca tattatatgt
      361 aaatgtcaat agtaaagtgt cagaaatctt cctgattatt cttcctcttc aatcggcata
      421 ttctcccagt tgtggtaaac gttctgaacg tcgtcatcgt cctcaagatg ctcaagaagc
      481 agacccatct tcttgatagc gtcctcgtct tcgatagttg tgtagtttga aggaactttc
      541 tcagcctcag cagaaagtac cttgtaaccc attccctcaa gagcttctct tacagaagaa
      601 acctgattag ggtcgcatga tatctcgata acatcgtcat caacgttgaa atcgtcagca
      661 cctgcctcaa agcagtcctc cattatcttg tcctcgtcag caccgttgtt ctccataaca
      721 acagtaccaa catcagagaa catgaaagat acacagcctg atgtacccat gtttccgccg
      781 aacttgtcga agaagtgacg gatatcgcct gctgttctgt tcttgttgtc tgtaaggcac
      841 tcaacgataa cagcaacacc gcttggaccg tagccctcgt ataccattga ctcgtagctg
      901 ttcttgtcgc cgtcgcctgc tgcctttttg ataattctat caatattatc gttaggcacg
      961 ttgtttgact tagcctttgc aataagatct ctcagctttg cgttacctgc aggatcagca
     1021 ccaccctgct taactacaac agcgatctct ctgcctatct ttgtaaatat cttagctctt
     1081 gcagcgtctg ttgcaccctt ttttctttta atgttttccc attttgaatg acctgacatt
     1141 tgattcactc cttgatgtaa tattattcat tgatgctgcc cacagaccat gctgcctcga
     1201 acagttcacc tatacgttcc tcacgccccg tgagatataa aaaaccgaaa agacattcca
     1261 aacctgttgc catgcggtat tcccccacag tggaatgttt cggagcattt atgccctttg
     1321 catttctgcc ccttttgaaa atatccattt cctgttcgtt aagcagccca tcgtcaagcc
     1381 acttgtgaac agcctttgcc tgatattccg cacacaccct ctcaacagtg agcttgtgca
     1441 gcttgtccgc aggcatatta gctccaagga taagcttttc tcttaccctt atctcgtaaa
     1501 cgccgtcccc gataaaagcc agtgccagag gggagtattg catagcgtcc atatcagaaa
     1561 gctttcccgt gacgctccct cctatctgag atattcaaac tcaaagtcga atatagaaac
     1621 agtgtcgccc tcttcgatac ccatttcttc cagcttgtcg attatgccgc tggtcctgag
     1681 tacgttctgg aaatactgca gtgaggagta gtcgtccata ttagccgtcc tgagtatgtc
     1741 aagaagccag tctgcttcca cgaaataaac gccgtcctcc acagtaacac ggaagtcttt
     1801 ctttgagatg agcttatcgt caagctcctc ctgagtgagc ggctgagctt caaactgctt
     1861 aacaggcggc agaacagaaa gtcgctccca tacgccatac ataagctctt ttgtaccctt
     1921 tgtggtagcg gcagaaatct catagaagag aagtccctga tcctcaacat agtttctcag
     1981 tctttctatc tgctcagggg tagccatatc gctcttgttt gcggcaacta tctgcggagc
     2041 tttcgcaagc tccattgaga agttttcaag ctcgtgattt atcgccttga aatcctcgat
     2101 aggatctctg ccctcgctgc ctgaaacgtc gataacgtga acgataagtc tgcacctttc
     2161 aacgtgtctg agaaattcgt gaccaagacc gacgccctcg ctcgcaccct cgataagacc
     2221 tgggatatcc gccataacaa aagattttcc ctcttctatc ttaacaacgc caagcacagg
     2281 cgtcagggta gtgaagtggt agttggctat ctttggcttt gccgctgata caacagagat
     2341 aagtgtggat ttaccaacgt ttggaaagcc cacaagtcct acgtctgcaa taagcttgag
     2401 ttcaagcatt acctcatatt cgtcaccacg gaaaccaggc tttgcaaact tcggtatctg
     2461 ccttgtggaa gtggcaaaat gagcgtttcc acgcccaccc ttgcctccgt gagcgataat
     2521 agcaggctca tcagtagaaa ggtcggcaag tatcctgccg ctttttatct ctttaacaac
     2581 agtgcctcta ggaactttga caacaaggtc gggagcattt ctgcctgagc agtttttgcc
     2641 gccgccgttc tgtccctttt cggcaacata ttttctctta tatctgaaat ctataaggtt
     2701 tgagatattg tcgtcaacaa cgaaaacaat atctccgccc ttaccgccgt caccgccgtc
     2761 cggaccgcct gccgcaacgt atttctcacg gtggaatgaa actgcaccgt caccgccgtc
     2821 accggctttt atatagatct tcgcctgatc gacaaacatg atcaaacctc cttataaatg
     2881 atattatgcg taaatttagt tgtcattaat caacaaaaga ccccaagcct aaggctcagg
     2941 gtcatatgtt acaaaattaa gccttgggat aaaccgaaac cttcttgcgg tctctaccga
     3001 gtctttcaaa tcttacaaca ccatcgcttg ttgcgaacag agtatcgtca gaacccttct
     3061 taacgttctc gcctgggtga atgtgagtac ctctctgacg aacgaggatg ttaccagcca
     3121 gtacgaactg accgtcagct ctctttacgc caagtctctt tgactcagaa tcacgtccgt
     3181 tctttgtaga acccattccc tttttatgag cgaaaaactg cattgaaatc ttaatcatct
     3241 aaattacacc tccataaaat gtaatccgtc cgatcagtta tctctgaccg tgactttgat
     3301 cctgcctgaa aattcctcag ctagaaaata taaatgagtg agaagcccta aaagcagctt
     3361 gtccccctcc tctgagctgt cattttcaag ccttaatatt atttcgtttt cttccactgc
     3421 gacatcggcc tttatcttaa aagcctctgt gacagtgtta gccgtcaaca ttacagcaga
     3481 cgatacgctt gcacagcata cgtcctcgcc ataatctgca taacctgcgt gaccagttat
     3541 acgaaatccc acaagtctgt catgctcatt tctgaaaaaa tcagcacaga tcattatgcg
     3601 ttgatagctt cgatcataac cttagtgtaa ggctgtctgt gaccgatctt ggtcttttcg
     3661 cccttctttg gcttgtaagt gaatacagtc accttcttgg acttgccgtt cttgatgacc
     3721 ttagctgtta cggaagcgcc gtcaacatat ggagcgccaa ccttgatacc gtcgtcagcg
     3781 ccaactacca ttaccttatc gaaagtaaca gcgtcctcag cgtttacgtc cagcttttct
     3841 acgaaaacct catcaccagg cttaacctga tactgctttc cgcctgtttc aattactgcg
     3901 tacattacga atgcacctac ctttaaataa actcgctacg cacggtggcc tgcaaataca
     3961 aacacttatg accgtgaata tgcggcttta atatcataac atatccgccc caaaaagtca
     4021 agcacagctc tgttcctgct tgaaaattta cagaaattta acttttaaaa acccctttac
     4081 tatccaatga cgtcccaccg taaacatttc tccttaaatg taggtttgtc aatcatattg
     4141 gacactatta ttaagaaatt cgataggcg
//
LOCUS       DAWEMR010000210         4137 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7700_length_4137_cov_9.168055, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000210 DAWEMR010000000
VERSION     DAWEMR010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7700_length_4137_cov_9.168055"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3557..3566
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1266 a          863 c         1013 g          985 t
ORIGIN      
        1 atggttagtg ttcgtatgat gttgggtata tgcaataccg agttacgatt atatgggctt
       61 ttgatgataa tgtgatgaga aaaatgggtc tccacggatc atacaataca aattatcaag
      121 agttcatgta ttccgctggc ttactgacta ttatttcaga taatattgaa ataaatattt
      181 ccaaggagaa ttgaaatggg aacactaata tccgacaaat acgccgcatg ggagcaggaa
      241 tgcaccgagc gtttcaataa actaaaagcc aacgaggaag aactcaaccg cattttcatc
      301 gacatttacg gcttgcagga cgagctgaca cccgaggtgg aggataagga cgtgaccgtc
      361 agaaaagccg atctacagcg tgagataaaa agcctgataa gctacgccgt gggctgttgg
      421 tttggcagat attcgctcga tgtcaagggg cttgcctacg caggcggtga gtgggacagc
      481 acgaaataca agacgataat tcccgttaag gacaacgttc tgccaatatg cgacgacgat
      541 tacctgccca ccgacctcac tgggctgatc gtggattttg tggcgaaagc ttatggcgag
      601 gacacgcttg acgaaaacct gaaattcatc gcagatgccc ttggcggcaa gggtacgccc
      661 cgtgaggtga tacgcagtta tctgctgaac ggtttctatg ccgaccattg caaaatttat
      721 cagaaaaggc cgatttactg gcttttcagc tccggcaaaa agaacggttt caaggcgctt
      781 gtgtatatgc accgctggga gcgcaccacc gttgcgaccg tccgcaccga ctacgttcac
      841 gagttgcagg aacgctaccg cacacagctt tccttgcttt ccgagcagat agattcggtg
      901 gcacagtccg accgtgtgaa gctgaaaaag cgtcaggaaa agctgaccgc acagctcact
      961 gagataaaca cctacgagga gaaagtccac cacctcgctg acatgatgat agacattgac
     1021 cttgacgacg gtgtgaaggt caactatgcg aaatttgcgg acatactgga gaggatcaaa
     1081 tgacgattga taatattaac cgcagtctgg cggagcattt tgcgaaagat ctgcccgact
     1141 gctacgaccg caggatcata ttttggttcg attccgagcg tgagtttgaa tccatgcttg
     1201 atgaacttga cctgaccgat gtgaaaatat taaagctgac cggtgacaat ttttttgaag
     1261 cgaaaatgct actatctgag acggacacgc agtcgaatta tcttgtatat gaccctctga
     1321 cctacaaaaa gcgtgaggac aactggctta gggatataca gttatacagc gaggaatttc
     1381 gtgccgatct cgtttccatg cagatgaacg aactgaaaat gccccagaca acacagctcc
     1441 gccgtgccat gaagcattac aagaaattct ttgagagcaa ggagcgtgta ggcaagctgg
     1501 cggcactcgc cacaaaatat gaaaccgcag gacagttgca catcgatatc atggcggtgc
     1561 tttcgggtgc aaaacataat accgtcaatg gtgttatacg tgcgatactc tgcgattcgc
     1621 ttgacaatga agaaaatggt gcattggagc gtatcgcaaa atttggaagt gaacaggcgc
     1681 tgcaagaaat gacagcgaag cttttcggct tttcaaatga aaaaataccg ctgtacgatc
     1741 ttgcaacaca tatcctgctg acggcatttt cgaccctcgg tgatgaccgc gtgacttcgg
     1801 ggctggaaca gtatatctca cctgaaaaac agaccgcctg ctatgctttt cttgacgatt
     1861 ggaacaattc tgacgacagc gaaaagctgt tcgacatggc ggataatatt tcaaggcact
     1921 ataacctgac agatcgcctt aaaaaagtca atacagatat tctgctgaaa gccgacagcc
     1981 tgccttgcat tgatgaggta ataatcggca gatttatgag cgagatcggt gaggatatta
     2041 tcaaggctga ggagatactc aaagctgtcg aaagccgccg aacatcaaaa tggtatgaca
     2101 gatattccta tctgtatgac gggctgtatt atatcgccaa aatgcaggag ttccactacg
     2161 agcatattac gggatttcat tatggcagat atgacgacct gtggaaagcc tacggcaaag
     2221 acctgtacat catggataca tattacagac gtctgcatat tgcgttccgc aagagtcttg
     2281 tcagcacatc ggggacgctt gatgatctgt tcaggggtgc aatcgtgact gcggaaaaaa
     2341 tatacaagaa ctggtatctt tccgagctga atgggcagtg gtgtgacctg ataaaggact
     2401 atatttgtga tggctttgcc cttgatggta tttcatatca gaaggatttc tataaatcaa
     2461 atattaagcc gatagcaagc gacagcagag tattcgtgat aatatctgat gccctgcgtt
     2521 atgaagtggc gatggagctt aacgaaaaac ttcttcgtga aacaaatgga acagcaaagc
     2581 tgacagcgat gcagtctgta ttcccaagcg ttacgaaatt tggcatggca gcacttttgc
     2641 cacacgatga actgaccgtc aacgatgata tgaagatact ctgcgatggt ctgccgacag
     2701 aaagcaccga aaaccgtgac aagatactga aacgttgttc acctaaaaac tgtgccataa
     2761 catatgaaaa tctgctttcg atgaaccaga cacagcgcag agagcttgtc agcggtgcgg
     2821 aggtcgtgta tatatatcac aacaagatag atgctgtagg tgataaggcg aacacggaaa
     2881 agcaggtatt tgaagcctgt accgattcag tcgaggaaat gaaaaatctt gtgcgaatgg
     2941 tcgtaaactc catgagcggt tcaaatatca tcattaccgc agaccacgga ttcttgtatt
     3001 ccaatgagcc gctttccgag agcgatatgg ctgaaacgag ccttgtttca gggaacatat
     3061 ccgagacggg cagaagatat atcatagctg acaaaaacag cagttcggat attctgatga
     3121 aaatacctat ggacgagtat tctgaaagca ttaaaggttt tgcaccttat gaaaatattc
     3181 gcatcaaaaa acagggtggc ggcatgaatt ttgttcacgg tggtatttcc ctgcaagaat
     3241 gttgtgtgcc tgtcatcata ttcaagaata tatcaaagaa ttcaaagaaa ttcgttgata
     3301 taaagaaagt cacgttgaaa cttatcagca tgacaagaaa gatcagcaac aatatattct
     3361 cgcttgactt catgcagccc gaagcagtgg gtggcaagat cgttcccgca acgtatgaga
     3421 tatatctgtg tgacaagcag tcagttcctg tgagtgatgt ccagacggta acagccgaca
     3481 agacaggcga accgcaggac agagttttca agataagatt cacgctgaaa agtgcggatt
     3541 ttgacggcaa tgcggtnnnn nnnnnnggca catgggtaac tctgcaagga actaccaaac
     3601 atgaagtcca aactacgcgt gaccgtgaaa acagagaaac attaaaatca atcgttacag
     3661 tttgtgctat cggatcgaaa ctgctcttat cccccgaaaa gaaaaaacgc atttaccgct
     3721 ggcaagctgc tcaaaggcgg agagagcctg attattacgc tgccaataaa cagcttgaag
     3781 cagagatgag cattcctgta agaaccacat atggaggaac aatcatggat gattttgata
     3841 gaaaagtagt agacgcattt ccaggcaagt ctgtccgcaa ggatctgacc tcacttatga
     3901 aaaagggtgc aaatgtgcct acatatgtac ttgaatatct tcttggaatg tactgcacca
     3961 ccgatgacga tgatgaaatg aggatcggct tggataagat caagaagata cttagcgaga
     4021 attacgttcg ccccgaccag agcgattatg tcaagtccaa aataaaagaa aatggtccgt
     4081 atacggtcag cgataaggga actgtctggc tcgaggatga agagggtctg tgggtcg
//
LOCUS       DAWEMR010000211         4121 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7734_length_4121_cov_10.494097, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000211 DAWEMR010000000
VERSION     DAWEMR010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7734_length_4121_cov_10.494097"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1328 a          778 c         1045 g          970 t
ORIGIN      
        1 acgatctcat aagtgaaaac cttaacagta tgtggtacgg caacggcgta caccattgtc
       61 tgctgatcta cgacaagcaa cagggcgacg gcttgttaat agagtctgag ggtgctccct
      121 atgcaagata tgcccagtat attccaaacg ccaaactgtt gtacgaaaat catatgcaga
      181 atcatttaca ggaattaaaa ttttactgtc cgcttgaaat caacagagaa ccggaatgcc
      241 ggtatgacga ggaatacgaa aaaatctcgt cctatgaggc ttctgcttac aaatcggaga
      301 taaaccgatt tattgaggat tttacaaagc ccgaggagaa agaacggggt ctcatgaact
      361 ggtatggcaa gggcaattca gtcgaccaaa aggtacgctc ggcgtttatg tctgtcgagg
      421 agcgcgacgg agagcttgtt ggcgttatta cggctcagat ttacggtcag cttacagatg
      481 aagagcttga agaattccgc tcgtactgcg agggacaact ttcagacggc gttggggagt
      541 ctcttgaaca gcgaccgata aaaactcccg acggcgatat ttatgtcagc ttctggaatt
      601 cagatgacaa ctggagcttg cagacggagg aagaaataaa cagcattcag tcagaagatt
      661 tgaccgatga acccgatatt ggaatgacaa tgtgaggtac gcatggtata caatgaaaag
      721 aaagttgagt tgcttagaca gaggtatccc gagggtactc ggatttgtct tgaccacatg
      781 gaggacttat gtcctgtgga aagcggcacc aatggaacta ttattggagt cgacgatatc
      841 ggctcagtca tggttaagtg ggataacggc agaactttga atcttctgcc cgatgaagat
      901 aaatttcata cgatcaagca tgaacaaact cagtcagacg aacaaacaga agaaaatacg
      961 gaaaatgaag aaatcgcaga aatcgaggaa ttatcggaag aaccggaaat ggatatgtcc
     1021 atgtaggact tgaaatacgg cggtttatgt gatataatgt acaagataaa attctgagag
     1081 gaatacgttc atgagtaaaa acagtaagat ctgcccgaac tgcggcagaa aaatgaaaca
     1141 gcagtttatc ggattacagc attgtaagtg cggtataagc tggaaacatg atatgggtta
     1201 ctttgaacgc acaaacgata tggtattcgc tttgcagagt gttaaaatcg gcaagaaggt
     1261 aaagcaaatg cccattatac gaataaaaga gcgaaaggaa aatgaatatg ccgaataagc
     1321 cgctccttga ccgagcctta tacaaaaaaa ttaaagctat ggaccgtgaa acaatggaca
     1381 atttcattca gaatatatat cagacaggaa aagacgacgt acacgccaca gaagtcgact
     1441 tcgacaagct gcgtgaagat atatccaaga tcaagggtat cggagaaagc cgattgaatg
     1501 aaataatggc tgtgatcgaa agtcatatag gagctgactc cgaataatac aattaaatat
     1561 cgcagaactg ccgttaaatg agagatcatt tagcggtttt tttattgtca aaatcagaaa
     1621 ggggcgcttt gaatgatacg agtattcgac gcattttcag gcatcggagg cttccgatct
     1681 gctctggaaa gagtcggagg atttgaaatt gtggggtggt gcgagattga cagatttgcg
     1741 cagaaagcct acaaagcact gtacgatacc ggaggtgaac aattttatga aaatatcaga
     1801 gatattgacg tcggaaaact tgcagacttt gatctcctta ttggaggttt cccctgccag
     1861 ccgttctcgg tctgcggagc aagaaaaggc tttgccgacg agcgaggaga tctgttcttt
     1921 gagcttgccc gactgcttga agcgaaaaga cctaagtatt tttgctttga aaacgtaccc
     1981 ggtctcatgg ggattgactc gggaaaaact tttgcaaaaa tcattgaaac gctttctcaa
     2041 ctggggtatt gcgtggaatg gcgtgtgtat aacagcgccg attaccttcc ccaagtcaga
     2101 aaaagagttt acattgcagg atgtcttgga atcgactgtt ccggaaaaat actggctttc
     2161 ggaaaaagcg acagccagaa ttgccggaaa actgaacaaa tcataggcgg aagtcaaggc
     2221 acgagagttt atgatcccga tggacttgcg gtgacgcagt gcagcggttc gggcggtatg
     2281 ggcggaaaaa caggtctgta ctttattgac agcaatcctc cacctaatct cacagaaaat
     2341 gcaagatgta taaccgcaag gcagaacagc ggagtaagtc atcacaaagg tgaacactcc
     2401 gctgtttttt gtgatctgaa cgaaaatccg cagattacgg aaaacgcccg atgtctgcat
     2461 acaagaatgg acttgggggt aacaaacgga actcataaag gagaaagatc gggagttctg
     2521 attgaggacg gaccgagagc aattatcaat ccgtttaagg aaactacccg tcaaaacggc
     2581 agacgaataa aaaatcccaa cgaaccgatg tttacgctta cggtaaccga tcgacacggg
     2641 attgtccacc atggacggat aagacgcctt atgccaattg aggcatggcg gctgcagggt
     2701 tttacaacag agcaattcga aaaagttgcg gcaacgggga tgtccgacgc acagctctac
     2761 aaacaagccg gaaatgccgt aagcgtaccc gttgttgaag aaattgcgag aaatttattg
     2821 aaatttgatg aggagataaa ttaaatgaat aatatgatca agattttcaa aaatgaggaa
     2881 tttggttctc tcagaatttt aaaggatgat aacggcagaa ttatgttctg cggcaaggac
     2941 gtggcttctg cattgggcta cagcaataca aaagacgcaa taaagcggca ttgcaggtgg
     3001 ggcgtgaaac acgacctacc tcatccgcag tctccaagta aaactattaa gatgattttt
     3061 attcctgagg gagatgttta cagacttgtt gcacacagca agctgccgag agctgcggaa
     3121 tttgagtcgt ggatttttga taaaattctg cctcagatca atcaaacagg cggctacgtc
     3181 agcaacgagg aaatgttcat agaaaactat cttccgtttc tcgacaagcc gtaccgcaat
     3241 ctgttccgtt tgcagatgat ggctatcaac aagctgaacg agcggatacg tcacgaccaa
     3301 ccgttagtgg agtttgcgaa tcaggttgca aataccgata atctcatcga catgaacgct
     3361 atggcaaaat tggcaagagc cgaaaatatc cctgtcggaa gaaacaagct ctacggctgg
     3421 cttaagagta tgagagtgct tatggcgaat aatctcccct atcaggcgtt tatcgaccga
     3481 ggatattttg cggtcaaaga gtcggtattc gaggtcaatg gtctgaaaaa gacttatcag
     3541 cagacgtttg tgacgggaaa gggtcagcat ttcgtcataa atctgctgaa aaaatattac
     3601 gggaaggagg ttttgcaata atggagatca aaagcatttc tttacacgat ctgagaaaaa
     3661 tgaatgacag cgagggactt gtcctgcaag ggtgcggcgg cgatctgcag gaatgggtcg
     3721 acggcataaa cgatatgctg acggaaagcg gaatattgca gaatgacaat aggtttgaaa
     3781 aggcatatag ctttaaaaac ggaggcctta cctgtctgct gtttcccttt gaagatgttc
     3841 agcttgatgt aggtaagctt gcaatatggc ggctccgaac gagggaggat ttcggaagca
     3901 catggctgtc ggattacatt gtgaacaatc ttgaagaatg cgtttcggaa caggacgaag
     3961 atttagaaat ggagatgaag taatgcacac aaacaaagta aaagcgaaag ttgacttcaa
     4021 gttctgcatg ggaagtatcc ctgcaatgct gagagccaca aagccagtac tttcggaaag
     4081 gcagtataaa gaattatgta ctgaggttaa caaggtcgac g
//
LOCUS       DAWEMR010000212         4109 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7751_length_4109_cov_8.339665, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000212 DAWEMR010000000
VERSION     DAWEMR010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7751_length_4109_cov_8.339665"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    716..750
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1318 a          722 c          868 g         1166 t
ORIGIN      
        1 caacaatcag taatataatc aagtaattgg tatttagagg agttgactgt catgcttaaa
       61 ggaattaata aacaaatcat cgaaataaag tgtacagaaa atgaatgttt tgataagatt
      121 ctattatttg taaacgcaaa taaaaatcat cttcatcctg atattttaag aaaaaatgct
      181 gttgatatcg caagtgcgat aacgggggac aaaatgtttt cgacaaacag aaaattcaac
      241 tttgcggcag tgatatcaac tataatgggt gtggcaatgc ttgttctgct gcttatcgca
      301 ttgtgtatgt gataagcata aatatcattg ataaaatttg tgtaaatcgt cgaaagtcta
      361 tataaaaagc ctgtttacta ttggcattat gttttttttg tggtataatt acgagtagta
      421 tatttttttg aaaaggaaaa ttcaatgagt aattatcaag ataaaaaaaa taatttagac
      481 gaagaaaatg aactgcagct tataattggc agaaaccctg ttatcgaggc tttaaaagca
      541 aataaactga tcgacactat ctatattaac cctgaggcta aaggctctat aacacttatt
      601 tgtagacttg caaaggaaaa aggcataaat gtcaagcgtg ttaccgaaac aaaactcaac
      661 aatatgagca ggggagcttc acatcagggt gttattgcta tgggtgcgtg tgcggnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaacgtccg caggtgcagc aagctggttg
      781 cctgttgcaa gagtttcaaa tcttgcagca actattgata tgcttaaaga aaacggtgtg
      841 tggatatacg gaactgacgg cgagggacag agctatactg aagttaagct aactggtcct
      901 atagggctga tagtcggctc tgagggcttt ggcatgggac gtttaataaa agataaatgt
      961 gattttttgc ttagtttgcc tatggcaggc aaagtcaatt ctttgaatgc ttccgttgcg
     1021 gcgggcattt ttatgtatga agccgtaaga cagagaaatc tataaggaac agggaggtta
     1081 aagctttggc tgataaattc tcaatagacg atataatttc agaatacagc gaaaaagttg
     1141 aaaacgattt aaaaactgat gagtcacagg aaaacgatga aattttaaat gaagatacta
     1201 atgaagatac taatattgat gagagtattg aaaaagataa tgatatcagc gatatagata
     1261 gtgacggcta tgatccaata aaagcaacag attttgatga tgaattggcg tcagatcata
     1321 cgaacgaaaa aaaacgtaac gaggaaaatg ctgcacttat aaataatctc accaaacata
     1381 aaaaaggcaa ggctgcaaag cagagcattc cgcctgtgaa cagggcaagc cttaaagata
     1441 tcaaaatggg actgacggga aaaataatcc cgaaaacaga ggagtttgac aaggcactta
     1501 tccctgatga cgccacctat gaggaaaaat cctctatatt gtcacagcac agaaagaaga
     1561 aagttgacag ctttgttctt aaaactaacg agcaggaaag cgagcctgac agtgatgata
     1621 caaacgataa agacaaggca gggcagacgg agtttaagaa gtttgatgac gccgaaaagg
     1681 tctttggctc tatccgtcat gtgaagagca accttgttct aaggctttgt gtacttctgt
     1741 ttacgggcat tttcagcaca ttcataacgg ttgcaaatga cctggaactg cctcttataa
     1801 agacatttga cagaactata acccctgcgg catatctgtt tgcaaacact attttgggtc
     1861 ttatttctat agcggtgtgc tataacgtgc ttcttgcagg aataaaaagc ctatttaaaa
     1921 aagaggcaga taatgataca atagcagcga taggtatttt tattacagtc attgcaggaa
     1981 taataaatct atttgaccct gagtctattc gtgacaatgt ttaccatgtt tacatatcag
     2041 ttgcaatcgt gggtctgata ttcaatactc ttggaaaact tatggtggta aaacgtactg
     2101 agcgtaattt taggtacgtt gcaggagatt ttgagagata tgcagttaca tccgttgaag
     2161 atgaggaaat agcagaaaac ttcacaagag gtgctgttgc agagccaaag atagcagcta
     2221 tgcgcaaaac cgagtttgtg gacgatttta tgaagaacag ctacacttct gatatctcag
     2281 atgagtattc aaagaaaata agtccgtata tcctcattgc aggcgttgtt atagcacttt
     2341 tgtcacttat atttgataag ggtgcgtcaa acatgaaaga acgtttgttc atagcacttg
     2401 cggctttctc aggcactgtt accatgtgtt cttcgtttgc ggtaatgctt atagtaaatg
     2461 tacctctggc aagagctaca aagaagtatt tgcagtattc agcggtaatg ctgggatatt
     2521 cttctgttga tgaatttgct gacacaaatt cacttcttgt tgacgctgaa cagcttttcc
     2581 ctaaaggaag cattgagctt gcaaacctta agcttctctc agctatttct attgaagact
     2641 gtattttaat ggcggcaagc cttagctgtc aatcgggaag tgttcttaag tctacattct
     2701 acaaaatgct cagaggcaag actgagttgc tttatcctgt tgaaagctac atttatgagg
     2761 acggtcttgg actttcgggc tggatagaaa acaaacgtgt acttcttggt acaagagagc
     2821 ttatggaaaa ccactctatt gacggtctgc cgtcagaagc aaaagagaag gagtatacaa
     2881 atgggaatgt tgctgtctat ctttctattt ctggaataac agcagcaatg ttcgttattc
     2941 aggtatcgcc taatctgtcc gttacacgtt ggcttcagga gcttgaactt gaaggtatca
     3001 caacagtaat aagaacagtt gacggctttc tttcacagcg ttttctctct gacctctttg
     3061 atattgaaag cgacagcgtt aagcttctca gcttcagata tcacaaggat tacgaaagcg
     3121 aaaccgagta cgttccacgt caggcttcat caatgctttg cagcggacat tttccgtcat
     3181 ttgcaatgct tgtgataggc gcaaaaaggc tcaaatttac cgcagaactt ggtgcgtcag
     3241 tgatgtatgg tgcatcgata cttgcggcgc tcatagccct tataatgatg ctgacaggtt
     3301 catttgttca gcttactcca acgctagtta tcgtctacaa cctcattgta atgggagtag
     3361 cgttgcttat acagcacatc aaaaaactat aaaaaaataa aacctcggtc tgtagtgaaa
     3421 caaaccgagg ctattttttt tacttattgc ttattgtcca atctattggc tgaatattgt
     3481 tacttacaag gaaatcgtta accttagaaa aatgtctgca tccgaaaaaa ccattatacg
     3541 cagacaatgg agatggatga gctgccttga atactgcatg aatagggttc gttataagtg
     3601 gtgccttagc cttagcattg ccaccccaaa ggataaacgc agtaggcaca ttacgctcat
     3661 taagcttttt tattacagcg tcagtaagct gctcccagcc tttaccctta tgagaattag
     3721 gctgaccctg acgaacggta agaacagtgt tcatcatcag cacgccctgc ttagcccaat
     3781 cggtaagctc accattatta gggatagaga taccaagatc agcattaagt tctttaaaaa
     3841 tattctgcaa agacggtggt ggattaacac cagctttaac ggaaaaacaa agaccatgag
     3901 cctgtcccgg accatggtaa ggatcctgtc caatgataac agccttgacg ttttcataag
     3961 gcgtgtactt cagtgaattg aagatatcat gcatatcagg ataaacagtg aagtgtgaat
     4021 attcgtattt caaaaattca cggagtttca aatagtattc ctttttaaac tcatcagcca
     4081 gaatatcgtc ccagctattt ccgatatgc
//
LOCUS       DAWEMR010000213         4106 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7757_length_4106_cov_6.728462, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000213 DAWEMR010000000
VERSION     DAWEMR010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7757_length_4106_cov_6.728462"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1281 a          609 c          815 g         1401 t
ORIGIN      
        1 ataattactg caaaacagat atatataagg gaaagatcat ttttgataat actgttgggg
       61 gagaacgatg aaaaatttcc atttagtaat gccaatgggc ggcaagggac agcgcttttt
      121 caatgacggg tatgtagccc ctaaaccatt gatcgagata catgacagac cattcttcta
      181 ttggtccact atgtcaatac tgaagtataa tccagattgt gatgttactt ttgttgtgct
      241 gaaagaacat atagagaagt tcgatataaa aaatcgtata ttatcgtatt tcccacaggc
      301 tagagtggag gtaatacctg aggttcttgc aggtgcagtg ctgacttgca tggcaggtgt
      361 agaaaatatc aatgacgatc gtcctatctt gtttaatgac tgcgatcaca tgttttgttg
      421 ctctgagttc aattctatgg atgaaaaaag tgcggcaaag tatgacggct tgttgttcac
      481 gttcaaatca gatctgccgc agttcagcta tgctttgtgt gatgaaaatg gtgttgttat
      541 tagaactcag gagaaagttg ttattagtga caatgcaata tgcggagcat attatttcag
      601 aaataaggac atttttcttg aaaacgccaa gagatatttg tcggagtgcg aatatagtga
      661 atattttatg agcggcgtgt ataacagcat gataaagaat aaacttatat ccggcgtagt
      721 gggtacggat tatcatatat cgtttggtac acctgctgag tatcaggacg cagttgacag
      781 actggaattt ggagagctgc tatgaacatt cttatgtttt tagctgcgct ggctgtgata
      841 actcttggac acttttttag aattaggcgt tggaaaagtt ttatatcggt ctatgaagat
      901 tcacatgatt cagacttgat gttttgcaca ggaataggtt atttggtgga taatgttctg
      961 ccattccatg tgggcgatat tgtcagagca gcgataattg gcaaaaaact aaaaaatggt
     1021 gctgcatttt ctcttgccgt gataatcata gataggatct tagatgtttt cgttgtagcg
     1081 ttcatttatg ggacgatttt ttttgcctca gggaaaaatc tgatgaattt tattttcttc
     1141 accgggtttt cagcactgct actgtttttt ctttggttgt cggttacatt tagcaaacgt
     1201 tttaaaaagt gtgtgcttgt attttcctca atatttaata cgaagatcca gctttgcatc
     1261 ttagaatttg tgtggtcttt tatctgtacc atacgcaata cagtgaaaaa gatcgacaaa
     1321 acaaagcttg ttctaagaac actttgtatg tggtcttgct acattttgtc atatctgatg
     1381 tattcaaatt gcttaaaaaa cacgtctttc gtagatgttt tcaataattt gttcagcata
     1441 gattcttatt ctccatttgt tgattatgta cgtcatggtt tttctcacta ttattttatt
     1501 ttcctgctgt ttaatttcct cacttgtgtg tctataatag tggtcgcatt ttttgaaaaa
     1561 ttcaaaaaat gcagctctga gaataaagga gagcttatta taccatatac aaatgaaaac
     1621 agctgtcttg actttttgaa aatatatttc tcagatatcc gtgataaaaa ctatattgac
     1681 cggtttcttg aaataaataa agatgtaatt atattgagaa actgttcagc agtttcaaat
     1741 gcaacaactt tgcaatgtat aaaaagcgga agaatggtgt atcggaagta tgcctttggc
     1801 agtgacggtg aaaaattatt tgaacaggta aagtggcttc aaaataataa agatcagctg
     1861 tatgttactg agatcctaga tgcgtatcag aaaaataatg tttgttacta tgatatgccg
     1921 tatcttggtg attctatagg tttgtttgat tacatacatt ctatgccttt ggagagttct
     1981 tggagaataa tggagagcgt agtcagcgat cttgaaagca attattctaa gaaatatagt
     2041 tccaaggctg acgctgatac aataaggcag tattatgata aaaagatcag gtccaacata
     2101 gataaaataa tgaatgcaca cgttttgagc gaattgacaa actatgaaaa ggttgtcatc
     2161 aacggtgaaa catatgataa tctgacaatg tttcttgaca aattatactc ctttgacttt
     2221 tggaaagaaa tatttgaaaa tgactattat tcagatatcc atggagattt gacagtagaa
     2281 aatattgtgt gcaatattaa ttatcccaaa gggtattatc tcatagaccc aaatggcgga
     2341 aatatccatt cgtcgcctaa ccttgactat tcaaagcttc tgcaatcgct tcatggaaat
     2401 tatgagttct ttatgcacac agcaaaggtc aaagttaata aaaatgagat atcgtttaag
     2461 ataacaagaa ctacatcgta tgatgtattg tataaaaggc ttgataagta tctaaaagac
     2521 acgtttgatg ccaagagagt caaaagcata tattttcacg agatagtgca ttggctgaga
     2581 ttgatgccgt ataaaataaa taatgacagt gatcgtgcag caatgtttta tgcaggcctt
     2641 gtaatggttg taaatgatat ttttgaggag tttgataata ttgacaaaag gatcggaatt
     2701 aaagcttgca atgtttgacc tagatggtac gctttttgat acgaacaagg caaattatct
     2761 ttcctataaa gaagcctgca acggcttttg tgatgtaagc gaggattttt tttacgagca
     2821 ttgtatggga aaaaactata agactttttt gcctctttta ggagtggagg ataaagaaaa
     2881 tatagaattg atacataaac ggaaaagtga taattataag agctttttct cttttatacg
     2941 tcctaatttt tcacttctga agatagctga actgtttaag acaaatggtt taaagatcgc
     3001 aataataaca accgcgtcaa gaagcaatac ttcagcgctt ttgaagtttt tcgattatga
     3061 tgattttttt gatctggtgg tcacacagga aatgacagat aagacaaaac cttttcctga
     3121 cgggtatctt tatgccatga actatttcca ttgcacgcct gatgaaagca ttatatttga
     3181 agattctgat gttggtttgc aagcggctct taaaacacaa actgcggtat ataaaatagt
     3241 acgcttttga agggtgacac aatgaaaaat aaaaccagaa atttgatacc tgtgcttgta
     3301 atagtgctgt attcaatatt gttctttgct gttattttgt caaagaatag aagttccctt
     3361 tatgttgatg ataatattct tcagtgggga cctgttataa ataaagcatt tgataatatt
     3421 ttcagcggta agggcatacc atattggaat ttttatcagt acaagggaat aaacattttc
     3481 agcagcggtt attatggctt gttgaatccc tttatgtatg tttcctatgc cataagccat
     3541 tacctgatga acgccttcct tgaaactctt attgtttacg aaataattat gtatattatt
     3601 ggtaatctta tgatgtataa gatactgaga acattagatt tgaagcgtag tacgataata
     3661 atagcgatgc ttacatattc aaccagcgtg atttttttcc ttttcagctt ttattatttt
     3721 actttcaata attatttctt tatgccgttt ttaatgtgga catttattca gaccaactcc
     3781 aaaaggaccc gctattttat tcctggcata gttttggctt tttccctgct aatgggccat
     3841 gttcagttca gttgttatta tgtaatggta tactgtattt ttcaagtggt cgcttctgtt
     3901 caaaagaaaa aattaaaaga attagttccg ctcatgacaa atttgatgat attcataggc
     3961 ttgtcaagcg ggataataat tgggtctatg aaagtgtcag gcgacagaag tctgatactt
     4021 tcaggcaatg agggcttttt cgccctgaac gtagctattt caaatatttt caagcctata
     4081 tgtatagttt tatttaataa taagac
//
LOCUS       DAWEMR010000214         4099 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7771_length_4099_cov_12.100643, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000214 DAWEMR010000000
VERSION     DAWEMR010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7771_length_4099_cov_12.100643"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1008 a         1025 c          849 g         1217 t
ORIGIN      
        1 tgtgccgctg tgtttgctgt acccaaaatt cagccaaaat agttattttt gcgatctggc
       61 ataaaaaaag agtcgagatt ttccaaaagc aggaaatctt gactctttga gcgctgtttt
      121 tttacaacgc cttttttgca tcttaataaa tgtaacgctt ttttcttaaa ttacctagca
      181 ggtgtacagt agcaaataga ataaactgtg atatcaccgt tttttgccgc agcatgaagc
      241 tgatccagct tatcaaaacc tgcaccaagc tcctttacac agtctgcata tgtgaactta
      301 gcatagtaat gatcattcgc tctgcctgtt tcgctaggct gaactcttgc ccagtttgct
      361 cctcctgacc agctctggag aataagctca agctcgttct ctgcgcccga atactcaaca
      421 tagaaatagc catttgcaga aagatacgaa acatcaagac tgccataatt ttttgaagtc
      481 ataactgaaa ctgcctgacc ccatgagcca catgattttg caccccagaa aatagataca
      541 tatggatcct gctccggctt atcagggtca acaggatcta ccggatcaac cggctctgtt
      601 gactttccga caatatactt aacactaaga acttcgatat ctccgttagc cgcaccaaca
      661 tggaagcggt ctactttaga gaaatctgtt gttccgaacg ccacagcaac atcgtcgtag
      721 ctgtactttg cgtatacaat gccgtccttt tctgttacct cagttggtga aactctcgcc
      781 cagtctgcac cgcctgacca actctggaga atgacctccg ccttgtctgt gccgcccttg
      841 aactgaactt caaaatatcc gtccttttcg ataactgagc tgtcaaagct gccgccctcc
      901 ttagttgtgg aaagtgtgat agcctgaccc cagcctgatg tctttgccga gcctgtgaag
      961 ataacagttt cttttttgtc acttacctcg ccagttgcag ggataacctc tgtctttatc
     1021 accttgccgc aggttgtaca ctcagtgtgc tttgagccat tttctgtcgc agtagccgcc
     1081 ttgtcgatta tccatgcgct ttcgctgtga cctgtcgcag ggatagtttc agtgtacttt
     1141 tcaccgcatg agcaagtgta taccttttcg ccgtcagttt cgcaagttgc agcctttgtt
     1201 gatgtgaggg tatagctgtg cctgtgaccg atagggtcgg tgtaatcgtc cttctttgtc
     1261 tcgccgcagg aagcgcatgt gtagatagtg tagccctttt cgttctctgt aggagcaact
     1321 gtctcgccct ttacccagct gtgacccttg gcagggatag tttcagtgta cttgtctccg
     1381 caagtacaag tgtaaattct ctcgccctct gttgtgcaag tagcttcctt tgttaccttt
     1441 gcggtgtagc tgtgagtgtg tgatgactta acattcttga cagtgagtgc aaatgtatct
     1501 gtgtttccgt ctgagaatgt gtatgtaagt gtgtaattac catttgcaag cgtcttgagc
     1561 cagctttcgt tgagcattat ctgacttccg cttgtagaat aatctaagcc gcctgtaagc
     1621 ttcttgccgt ttactatgat agactcaacg ctctttccat aaagcacaag gtctgttctc
     1681 acaccgcttg gagcatcagc gtcaaatatg cctgactttg tggaaaggtg tggcttctcg
     1741 atctgtccgt cctcgtagaa aacaacatcg tctatgtaaa ctgtacctgt cttttgagca
     1801 gaatctccgc ccatatagaa cgagaactgg ttaagctttg atgtatcaag atttccgccc
     1861 gactgccagc ttggcgcttt aaagtcagcg aatggtatct tgatagtttt tccccctgcg
     1921 aaatcaagtg ctatctgcgc ttcaaaataa ttgtcgtttg cgtctctaag ctgaacagta
     1981 aggctgttgc ctgtgccgtc cccctctacc caaagctcaa cgcctgcaaa tgatgaagca
     2041 tttcttactg caatagggtg gtttactccg cagtagccgt ttggcgagcc gatatcatat
     2101 ccgaaagcaa gcgcctttga accgttcttt gcagcaagag aaagtgaaac ttcattgccg
     2161 cttgtgtttc ttgaataaga cgaccaaagt gccttatcat atgaatagct ctcaaatgtg
     2221 tcaagctcat caaggcctgt ggtgtcggaa acatttattg taagctccct atcctcgccc
     2281 tcatagaaat cgaacttaag cgtcacaatg cctgctgaaa gcgtcttgag atatgcactt
     2341 ttaagggtca cagtgtcgcc cgaagtagtg taatctgtac cctctgcgag tgcttttcca
     2401 tcagcaacaa tgcctctgaa ctcgcttgtc ttgcgatcca tggtgatatc aacatcactg
     2461 cacttcttag ggtttacgtc aacagagatt atctcaggtg agataatagg cttagggata
     2521 gagctgtcgc tcaccgtcag attgaactta ggctgattgc cgcctgttgt gagtatcctg
     2581 cacgaatact ttgcaagctc ctgcctttta agataagaag ccttgatagt aacagcgttg
     2641 ccacttatgg tgtaatcgtc tgacgagagt ttctttccgc cgacctcaac accgcttatg
     2701 ctgccctctt tcatagaaac attcaccaca acgttacctg acttgcttct gtcaaaactg
     2761 tatgtagact tgtcagtggt gtttacaatg ttcttctttt ccatttttgc agcagcgttc
     2821 atcataagag ttcttgtgct gtccgtcttt tcgataccct cagggccgta aacggtgtag
     2881 ccgtcatagt cctgataaag agtgccgtca tcaagatagg aagctatcat ccagtagttg
     2941 aagccctgat attcgacacc ctcatagtag tcacctgtta caatatccag ccaatcggaa
     3001 taagcgatgt cacgctttgt tttgtctgta agaccgaatt cctcaaagat aataggcttg
     3061 tcagcggaag atgtggtctg ggcatggcgc tttatccact cgagatcatc atcgccgagg
     3121 tcaaagcccc actgatcaac gtacatatgc ggtgtgccaa agtctacagt gtcgatagtc
     3181 ataagcttgt caaagtcaac accgtagtag cctgaatatg cggaagacgg aagatcttgc
     3241 cttgcagctt cctgatagcc aagattataa aagccctcgt cgccaacaga taccatgtga
     3301 taagggtcga tagactttac atatgccgac atttcctttg cccagttata aagaatatcg
     3361 tccttgcaga actcgtcaac agtacatctc ggctcgtttg aaagctccca agaaaaaaca
     3421 gcttcagaat ccataagctt ctcgcctgtg tagtagttgg tgtgatttaa caaagtcttg
     3481 atataatcct tataccaacc cttgatagtt tcattagtgt aaaaatcgca ggtgtccttt
     3541 tcgtccactt tgctattgcc aacgctcttg ccctgtgaca tctgatacca ctttacatac
     3601 tgacccattc cgccgaacgc ttcccaatag ttcgtgaaag tgataacaag tttcatatca
     3661 tgttcttcag cctgcttgat aatataatca agatgtctca ggccgtcctc gccctcgttc
     3721 acaacaggct tgccagcctc atcgtcccaa tactggaagt aaactccgtc cttttcgccc
     3781 gttccgtcat tgttgccctc aaaggtctca tgaccagact gcgagtcgat ctcgcctgtt
     3841 ttcttaccaa cgtcaagatt tccccaaatt cttattactt taagccccat ttttgaagcg
     3901 tcatcgaaaa cattctttac ctcactcttt gacttatagg tgagataata gcagttcgtg
     3961 ccgccgtaat agtaagggct tccgtcgcac ataaactttg agccgtcagc ataaacaaat
     4021 ccctccgggt gtgtcgccgc atactccgca tatgcgctga tatctgcgaa aggcacaatg
     4081 ctaacagcca ttgcagcag
//
LOCUS       DAWEMR010000215         4020 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7920_length_4020_cov_7.401765, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000215 DAWEMR010000000
VERSION     DAWEMR010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7920_length_4020_cov_7.401765"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1188 a          879 c          876 g         1077 t
ORIGIN      
        1 attcctgcta ggtttttatg acagcttcat aaattccctt aacgacatta tgggacgtga
       61 caatgcaaag cgtaaaaaag catttttctt tcttataaag cttggcattg gctgggtgat
      121 cggtatgctt cttgcagtaa gcgttcttgc caacgtgttt gaaagcggaa tctacaaggt
      181 aagctcgctg tttctgggct ttatcattct ggcaataccg gtcatgataa tggaagaaaa
      241 agagagcatt aagggcaaat attacaacat catatgggct gttatcggca ttgctatcgt
      301 tgtgggcatt tcccttataa atccctccgg cagcggtgct gacgtttctc acatgaatat
      361 aggcacagtg atattcgttg ttcttgcagg aatgtgcgca atatctgcaa tggtgcttcc
      421 cggcatttca ggctccacca tacttctcac attcggtctt tatatgcctg ttatcacggg
      481 aataaaagag ttccttcatc tgaatttcga gtatttcgga cttctctgct ctttgggtat
      541 cggcattatc ctcggcgttt tcgttactct caggcttatc aaaaaggctc ttgaaaagtt
      601 cagaagccag actatctact gcgttatcgg catgatgata ggctcactgt acgccatagt
      661 tataggtcct acaacgctta aagagcctaa agaccctatg tccttcggca cattcagcat
      721 cgtatttttt atcatcggtg cggcgatcgt gttcggtatt caggtagcaa agagcgtgta
      781 tgacaagaaa cagcttgcaa aggctgaaga atagatatta tatcaaaacg aacgaatata
      841 taaaaaggct caatttctga atcatagaaa ttgagccttt tgctgcaaat aacgtaagta
      901 cacttttttc tgagcacata tcgtatggcc tatatgccca cctagcaaat tcatattctt
      961 gtgattatat aaaaactacc gcccctactg cccccttttg ctgttcattc ttccaaaatc
     1021 agcattttat acaattgaac gccttgacaa ttatgcaact ataccatata ataaaattag
     1081 gcaaatgcaa tatcctgtat tatattccaa acataatatg acaactattg cagaaatagc
     1141 atatcagcgt ctatgatttc tacaaatcat acgcctgccc tatgtagaaa gtgtttaaat
     1201 tttaggggcg tttggcattt tttcttgcaa aaattgtcgg cttgtggtat aattatcagg
     1261 agcaggggcg tgttattccc ccaaacaggt gaaaaccatc cttatttctc aaacgttttc
     1321 gccaatacag taataaaggt gcagagcgca aagctcgact tgaaagtata taggaggtaa
     1381 agatttatgg caaatagtgc tgtagaaaaa acaatttcca cgcaggaact taaagaaaag
     1441 cttcttgacg ttcttcacaa cgatttccag acaacacctg aggacgcttc cgatcagcag
     1501 gtatacgagg ctctttctaa gatcgttgta ggcattctta aagctaagag aagaaagttc
     1561 actgttaaaa ctcagtctga gggcaagaag aaggtctact atctttcaat ggaatttctt
     1621 atgggaagaa gccttaagac ttctctttat aaccttgaaa tgcagaagca ggctacaaag
     1681 gtccttaagg acatgggcat ttctataaac ggcatttacg agtgtgagcc tgacgcaggt
     1741 cttggcaacg gcggtcttgg aagacttgct gcctgctatc ttgacgctct tgctgccgac
     1801 ggctatcatg ctacaggcta ttctatctgc tatgaatacg gtatcttcaa gcagaagctg
     1861 gaggacggct ggcagaccga gcttcctgac aactggctcc cgggcggctc tgtatggctc
     1921 gttcctgttc cgtcaaaggc tgtcgaggtt cgttttgacg gcgagcttaa ggaatactgg
     1981 gacaatcagt atcactgcgt aactcacgag aactacacat cagttatggc tgttccatac
     2041 gatctgttcg tatcaggcta caacagcgaa gcagtttcaa agctcagact ttggaaagct
     2101 gaaatggctg actttgatat gtccatgttc aatcagggcg actatcagaa ggctcttagc
     2161 aagaacatca tttcacaggc tatcacaaag gtgctttatc ctaacgacaa ccacgcagag
     2221 ggcaagtcac tcagactcag acagcagtat ttcctttgcg ctgcttctat cggcgatatc
     2281 gtaaaccagc acatgagcgt ttacggcaca cttgacaatc tacatgaaaa ggtagctatc
     2341 cacatcaacg atactcaccc gacacttgct atcccagagc ttatgagagt tcttcttgat
     2401 gattgcggat attcatggga caaggcttgg cacatcgtta cgaacacatt tgcatacaca
     2461 aaccacactg taatgccaga ggctttggag aagtgggact gcaacctcct caagagcgtt
     2521 tgccctagaa tcttctctat cattatcgag ataaacgagc gttactgcaa ggatctttgg
     2581 gagagataca aggatcaggc taaggtttca catatgtcta tcgttgagga caacaaggtg
     2641 aaaatggcta cactttgcgt acacgcttgc cacagcgtaa acggcgttgc aaagatacac
     2701 tctgagatca tcaagaagga aacattcaag aacgagtatc ttgacacacc aacaaagttc
     2761 aagaacgtta caaacggtat cgcatacaga agatggcttt atcagtctaa cgagggtctg
     2821 acagatcttc tttgcgagaa gataggcgac ggcttcctca agaatgccgc tgagctttct
     2881 aagcttgctg tattcaagga cgacaaggac gttcttgaca gacttgcaaa gatcaagctt
     2941 gacaacaaga agagttttgc aaagtatgta aagaatcagt acggcgttgt tctcaacact
     3001 gactctatct ttgacgttca ggtaaaaaga cttcacgaat acaagagaca gcagctcaac
     3061 gctttgaaca tcattgcaca gtacaattac ctcaaggcta atccaaacgc tgattttgta
     3121 ccaaagacat atatctttgc tgcaaaggct gctcctggct actacatggc aaagcagatc
     3181 atcaagctta tctggaacat ttctcaggag ctgaaaaagg acaagaagct caacgagaag
     3241 ctcaacgtta tcttccttga ggactacaac gtatcacttt ctgagatact catgccagcg
     3301 gcaaacatct ctgagcagat ctcacttgca ggtacagagg cttcaggtac aggtaacatg
     3361 aagcttatga taaacggtgc tatcacactt ggtactcttg acggagcaaa cgttgagata
     3421 cacgagcagg taggcgacga caacatcatc atcttcggta tgaacgttga cgaggtaaac
     3481 gcttgcaagt caggctacaa gcctatggac atctacaact ccaacgcagt tgttaagcag
     3541 gctatcgaca cacttcagta cggcatcaac ggacagcagt tcaacgagat cgctgacagc
     3601 ctgaagaatt ctgacccata catggctctc aaggacttcg attcttacca gaaggctcag
     3661 gcttacgctt ctgagtgcta caaggatcag acaaagtggc agaagatgag ccttgcaaac
     3721 attgcaggcg caggcatttt ctccgcagac cgttctgttg aggactacgc tagagatatc
     3781 tggggtcttt caaagtaatt tattaataca aaaaattatc ggacagttgc ttggtgcgac
     3841 tgtccgattt tttgtacata tatgcaattc atgaaacgat aaatttgtaa gaccacaaga
     3901 tgagcgaaag gaacgggacg ccgagggcgt cgtcccctac atggttcgag cgatgtttat
     3961 acaatgtgaa aatcaacata aatagtgacg tgcgagaaaa gcgggctgtc gagggcgcca
//
LOCUS       DAWEMR010000216         4002 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_7961_length_4002_cov_10.349379, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000216 DAWEMR010000000
VERSION     DAWEMR010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7961_length_4002_cov_10.349379"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1076 a          956 c          730 g         1240 t
ORIGIN      
        1 tactttaaaa gagcgtgata cacagtaccg tcgctaccat ctattcctaa ctcccatcca
       61 tttacatttt caaagaaatt tctactgtct ttaacatttg gcaacacaga tgcaacgctt
      121 tcttcaaagc taatcatcat ttcattttct actaatatcg aaccatttgc tgcgtttacg
      181 cgctctgcca tatgctttgt tgcatttgga tgaactttaa ctttattacc attcacttca
      241 tatcctttta attcaaatga ccttggaatg tcagttccat ctcttacacc tgaatcggaa
      301 gtaatattat ctttccaatt atcagataat tcaggaaccg tacattcatt atgcaccagc
      361 accccacact caccaacaaa gtaagtatgg aaatcctgca cctcaaagtt ataaaccttg
      421 ataggtgatt cgagaatttc gtgctgaacc cattcaactg ttacaagttc accatttgaa
      481 agtacaagta catcgcctgc tcgaagtgaa ccagctaaag tccaaccaag ttattgatag
      541 caattatttt tacaatccta attatactat aaaatctgta aaaaatcaat atccgaatac
      601 agaaaaagca gacgtggcaa aacgtctgct cagctatttg gagcaagcaa cgggaatcga
      661 acccgcatcc caagcttggg aagcttgtat actgaccatt gtactatgct tgcaaaaagg
      721 caatactaaa atatattgcc ttagagcaaa gcccattcac cacggacttt gctcattctt
      781 gtatttttta ttgtatctca tctttaaacg ccaagatgtg aaccccacga aagccccaag
      841 agtgttgttg aaaacatcat caaattcaca aagccctctg cctgtgatat actgcgttat
      901 ctcaatacct gctgaaaagg caagggacat aagaaaaact tttttgaact ttgtgacgtt
      961 taaaagatac ggcagcaaaa cacccaaggg gataaacatt gcaatgttac cgaatatctg
     1021 taatagccaa aaatcccgca gatacttgat gcttatgttc tccaaaaggt ttcgcacctc
     1081 ccagaagggc ataagctccg cctgacgctt gccccactgt ctgtctatga gtgtgagcca
     1141 gaggacggcg aagatgtaca ctgcaaacag cgatgtaagt atttttctct ttttactcca
     1201 ccgtaacgct ctttgcaaga tttctcggct tatcaacgtc atagcccttt cctactgata
     1261 cataatatga caaaagctga agcggtacta ctgaaaggct tgccgcaaaa aggttatcta
     1321 tccttggagt gtaaactgtg aagtcagcaa cgtcctccat tgcataattc ccataggtag
     1381 taagtccgaa aagctttgct ccacggctct ttacctcgac catattgctt atggtctttt
     1441 cataaagtgc agactgagtt accacgctga acacgacagt tccgtcctcg ataaggctga
     1501 taggaccatg tttaagctcg cctgccgcat atgcttcgga atggatatag cttatttctt
     1561 tgagtttcag gcttccctcc atacctactg catagtcgat acctctgccg ataaagaatg
     1621 cgtcctttat attcactacc tttgaagcaa accactgcaa gcgctcctta tcctcgagaa
     1681 ttttctctat cttctcaggg atagtattga tttcctcaac gagctgagca aatctttcgt
     1741 cggatatctc ccctcttgct tttgcaaact gcattgcaag gagatatgcg gcaacgagct
     1801 gacagctata ggctttagtt gttgcgacgg atatttcagg acctgcgagg gtatagaaga
     1861 cattatcggc ttctcttgcg atcgatgagc caaccacatt tacgataccg agagttttta
     1921 ctcctttttc tttacaaagt cttaatgctg cgaggctgtc ggcagtttcg cccgactggc
     1981 ttatgatgat agcgaggcta ttttttgcaa gcttcatttg tctgtatctg aactcagaag
     2041 caagctccac acggacagat agtgaagtca gttcttcgat aacatactga gccaccatac
     2101 caacatggta ggcagagccg caggcaacta tatacacctg ctggatattt ttcatatcct
     2161 cgtcagagag tccgacgctt tcaaagctaa tatttccatc tgtaaggaca gaattgatag
     2221 tatcctttat tacctttggc tgttcgtgta tctctttgag cataaaatgc tcatagccgc
     2281 ttttttctgc agcttctgcg tcccactcga tagttttcat ttctttctgt atttcttcgc
     2341 catcgagatt atagaaagca actgcgccct ttgtaagttt tgcaagctcc atattgccta
     2401 tatagtagac atttcttgta tacttaagta ttgcaggaac gtcagaggca aggtagcttt
     2461 cgccgcctgt aacgcctatt atcatagggc tgtcttttct tgcggcatat atttcaccgg
     2521 gatagtcctt gaacataaca gcgagggcat aagagccacg gatacggacg agggcgtgat
     2581 taagagcgtc aacaggagta cccaggtact ttttgtaata atagtcgata agctttacgg
     2641 caacctcggt atcagtttct gacttaaagg tatatccttt tttgataagc ttttcacgaa
     2701 gctcctgata gttttcgata ataccgttat gtacggcgat aacattttca tcgtcgctac
     2761 aatgagggtg ggcgtttaca gttgacggct cgccatgggt agcccaacgg gtatggccta
     2821 taccgcaagc accttttaca gccttgccgt cgttagtcat ttcctttagg actttcagtt
     2881 tgcctttagc ttttacgatc tcaggctctg cgtcaccgtt tcttacagcg ataccggcag
     2941 agtcatagcc tctatactcc agctttgaca ggccgtccaa gagtatcggg gctgcctcgt
     3001 tatttccagt aaatccaaca attccgcaca taacagtgtc tcctttaagt tttatttttg
     3061 ttgatgtaat cttctatagc aggtctattt ggagggaaga tatcctctgg caggtcgcag
     3121 atatcaataa atttaagctg cgacacctca gaagcctgca ttttaagctc gcctgtatac
     3181 tcgtggcagg tgtagactat atcaacgttt gacaccttat caccgtttgg gtaagtatac
     3241 tgccgtttta cgccagtcta gccgccaagc agttcgagct tttttgcttt aagacctgtt
     3301 tcctcaaaaa gctctctttc ggctgtttgc tcaacagact ctccaagctc caccgagccg
     3361 cctgcatatc cccagcttcc gcagtctgtt ctctgctgca tgagcatttt acctgcttcg
     3421 tttattacta ttacgcttgc tcccacctgc agcagaggga tgtggcctag gtgctggcgg
     3481 agggatttta tatattctga catatttgta cctcacaatt tcgtcaggtt catcctttgt
     3541 aagccagaac ataaagtcat cgttatcaaa atctagacat tatttttaac ctgacagttg
     3601 aagttttttt acttcatatc catacatctt ggcaagtttg aagtccaaca ttgcctttat
     3661 atcactcaca ttacatatag ttgttcatca atcccttact acctttgtca ctaacctgtt
     3721 tagctggcac acctgcaatt gtaatatttt ctttcaaaaa gctcttgcaa acaaccgcat
     3781 ttgcaccaat tgctattcca tttgcaattt ctatatctcc aaatatcttt acgcctggcg
     3841 caatataaac attatctcca atttgaggaa ctcgtctttc aacgccagct gcagtaccta
     3901 tattaacacc cacgtgaatt ctacaatttt caccaattct agcatatgga tttactataa
     3961 tactcccttg atgtgctatg cttagtcctt ttccaaatac at
//
LOCUS       DAWEMR010000217         3936 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8091_length_3936_cov_11.451688, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000217 DAWEMR010000000
VERSION     DAWEMR010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8091_length_3936_cov_11.451688"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1297 a          813 c          873 g          953 t
ORIGIN      
        1 catatgagaa tgagaagaaa gaaaaatctt aaggcacgtc ttgaagcctg cggagattat
       61 attttatatc ttgaccgtga cgagaagaat tttcaggaca aatcaaacaa acagcttatt
      121 gactttgaga agatcttcgg aaacagcgat ccccttgtgc tggagatagg ctgtggaaag
      181 ggtcagtttg caagagagat agccaagaga aatcctgaca agaatttcct tgcagtagaa
      241 aaaagcagta acgttatagt tgacgccgca caaatggcta tagacgaggg cataccgaac
      301 ctgagatttg caaggggcga ggctgaatat ctggattgct ttatccccga aaagtcagtg
      361 gagtgtatct atcttaactt tagctgtccg tatcctaaaa atacatatgc acgtcatagg
      421 ctcacatacc gagcttttct tgaaatatac agacgtattc ttgtggacaa aggcgagata
      481 taccaaaaga ccgacaatat gcactttttt gagttctcct tggaggagtt ctctgcggca
      541 aattacggac ttaaaaacat ttctctcgac cttcacaaca gcggcttcga gggcaatatc
      601 gtaacggaat atgagaagaa attctcagac atgggtatgc ctatctacag attggaggcg
      661 ttcgtaaaat aatgagcgag cagataaatc caattgtcag cgagaaagat atgggggctg
      721 acggcaagct tcgcaaatgg tcaacaggca gaaaagccaa gtggataatt tggatagtca
      781 taatcttagc agtagcactc ggcttctggc atcagcacta tatgcgctct gacagccaga
      841 taaaagccgt tttcgatgac aataaggcta actttcagac cactgcggaa tttatgatag
      901 aaagcatttc atgcgaaaag ccgacactac caaagggcaa atgctccatt aaatcgctca
      961 ctgaaaacaa tgcctgcaag tctgtgaaga aagagcttga ggagctggag cgcaggaacg
     1021 ttacatacat cgacagcgac ggcttgacag tgaaattcta taccatttat gatcattact
     1081 atatttaccg ctcacccata agctcatcag ggggcgagga taacctcggt gacgggtggt
     1141 cttatgtaaa aacaagtaaa agctgatttt tttatggacg aaaaaatttt tttcgtccat
     1201 ttttttatgc aatcaaacgg tctttacatt cgtgttatag atgaaaggct tttcaaaaca
     1261 acaaaggagg tgacagcttt gacaagagat attgactaca tagttgacaa attttcagac
     1321 gatatctacc gagcggcact ggcagtcaca ggcagcgttc acgaggcgga ggatatagtc
     1381 agcgaggtca taataaaata tttcacaagg cagggtgagc ttttcttcaa tgatgacgag
     1441 catcttaaag cgtggctgct gagaacggcg ataaatctat ccaaagacct gctaaggtcg
     1501 gcaggcagac gtctgcgtgc agaaaatgag gtaagtgcaa gcagcgattt ttcatcacct
     1561 gatatgcaga tagatgtgca aaacgctata aacaggctgg ataaaaaata cagaattgtg
     1621 ctttatcttt actactatca gggctataaa acagaggaga tagccgatat cctcggcaca
     1681 ggcaaaggca ctgtcacatc acggctaaaa agagcaagag gtaaacttag gctgtcactt
     1741 tcagactatg acgaaaagga gatgagcgta tgaacgaaag agatataaaa caatttttcg
     1801 caggcgcaag cctttctgag cagaaaaaaa cacagctcaa agaaacgctc aaaaagcgtt
     1861 ttccacaaca aaacggagat ataaatacgg atactttcac accacaaacc gagccgacac
     1921 agcctgccat agtcgcaaga cacaagggtg caaaggtagt tgcggcagcg gcggcagtgg
     1981 cgcttgtaat gtcaggcgga atttacatga caagaaatat ccatgactac gagccgaacg
     2041 caggcacagc tccacctgca aacagcagtg tatccggcga tacaggtaca ctgcagttgt
     2101 gttcagcaga ctttataaag cttgcagaca cagtgcttga aagcgcaaat tctacggtag
     2161 gaaatctcga cataatacaa aacagccctg catactgcga tatagtctac agcggcaact
     2221 atgacgaaaa tatcgagggc aaggaacaca gcacaaggta ttacgttgac ctgctggaga
     2281 aaaccgacag cggacttcca agcgtggatt ttttgacaga tgataacagc tatgagccaa
     2341 attctttggt aatcgatatc ctgtcggaga taaaaaccct tgatgaggac gcttttaaca
     2401 aggcggtgaa ttgcagaata gaagcccata ttgagaataa caactgcaag ataaccattt
     2461 cagacagcga taacagcgtt tctctcaaat acggaagcgg cgaaagcttc actgaaaatg
     2521 ccaaaattat agctcaaacc ctcaaaaatg acctgctgga tatctatgac gaaagctcta
     2581 tgaaaaaccc agacaatgcc atttttagct actcccctgc tgactataat tattacaacg
     2641 atatcatgaa cagcaacaat acaacagaat atgtaaagtc agactatgcg caaaatggat
     2701 atatcacaaa cgaaagtctg aacctcctga ccctgaatat cgtcaacgat tcggacatac
     2761 agttcaatat ggcgacagat tatttcactg tggatatgtt cacaaacaga tttactctgt
     2821 atagctacgc caatgacgga agctgggcgg tgtatgattc cgctccgttt tcaagcacca
     2881 gcactgtgga agctgacccg tcagccacgg cgcagtatat ttttgaaaag gcgcaggcgc
     2941 ttgtaacaaa gtataacacc agcggcaaga agcttcactt tgacaatgtg acctcactcg
     3001 accttgcgaa agagcttgca atgtcgtatg tgccagatat ggacaaagaa gataacatca
     3061 cgccggagct tttcgcatat ggactttggg tagaaactct gccatatgac aaggagtcca
     3121 gctcctatga tttatccgga cttgactttt caatatactt tgaaaaggat aaggacggca
     3181 gtgcatatga tgtaacccag gtggatgtca cagttgaggg ggatatggtt tattcataca
     3241 ccactgatga gcatgacagc gttgaaactt atgaccaaaa tgacagtgca ttatcttaac
     3301 aaaacaatgt tggtagctcg gttgctcact aaacaaaggt agagttaccg agccacaaaa
     3361 taatagctat actctaaaat agagtagcga gataaaaaaa caaagttagt caacaaactt
     3421 gctaattaaa caatggtaaa gtcaccgagc cacaaaataa aagctatgct ccaagataga
     3481 gtagcgaagt aaaaaaagaa aaaaagtgac ctgcaaagtt aattgcaagt cgcttttctt
     3541 tatgcaataa tatttatctg cgaattagtt gttgatgagc ttctcgtcat cattgttcca
     3601 gctgtagagc tttctgatct ccttaccaac cttctcagaa ggatgctcag cgtggagctt
     3661 tctcatagcc ttgaagtgta cctgtgaacc tgcgtcagac atatcaagga ggaagtcctt
     3721 agcgaatgta ccgtcctgaa tgtcagacag aaccttcttc atagccttct tagtgtcctc
     3781 agtaacgatc tttggacctg tgatgtagtc gccgtactca gctgtgttag agatagaata
     3841 tctcatgccc tcaaaacctg actgatagat aaggtcaacg atgagcttca tctcatggat
     3901 acactcaaag tatgcgtttc ttgggtcata accagc
//
LOCUS       DAWEMR010000218         3912 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8143_length_3912_cov_7.327716, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000218 DAWEMR010000000
VERSION     DAWEMR010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8143_length_3912_cov_7.327716"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2541..2550
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1292 a          645 c          891 g         1074 t
ORIGIN      
        1 atacttagaa atcttcttga caaatttgca tttgagtatg attggtcagg agtatcaaca
       61 ccagcaaagc ttactgttac ggagataact gcggctgaaa aagagaagca gacagagaaa
      121 accgaacctg agttttatcc tagtcttcca cggcttgatg atgagtttga taaactatca
      181 gcagcggaaa agggaacttt tacccacaaa tttatggagt tatcggacta tgataaggca
      241 catgaaagcg taaaaacaga gcttgaacgt cttgtgaatc aggggtactt cagccagaaa
      301 gaagctgacg gagtttatgt tgaagcactt gaagcattct ttagatctga tttttatgag
      361 cgtgtgaaaa attccgataa tgtgatgcgt gaaaagaagt ttcttgtttc aatgagcgat
      421 cttgacttaa gtgaaaatct tcctgaatat aatggtaaca gcggtatgct tcaaggtatc
      481 gccgattgta tcttccacga gcctgacgga tatgttattg ttgattataa aactgacaga
      541 tttaaagatt tgtcacagct catgggctat aaaacacagc ttgcactgta taaagcatcg
      601 tttgagatga tactcggaga gaaaataaaa agctgttata tttattcatt ctggctgcga
      661 aaaggggttg aaatcaagct ctgaatgtga tataatgaca tgtagagagc tttgataata
      721 tttttttgaa ataatttgag aaaaggtatt gacaagtggg taaaagtgta gtataatatc
      781 atggtaaaca aagcagaaca atgtccgttc tcaccttaag cgcagatctc tgtagtaaaa
      841 aggtcaacat gaatttgcat atcaatgtga ttttatgcgg gtacggatca gttctgtgcc
      901 cgttttattt tatttttgga ggtgctgcgt cattagcaac agggaacatc agatcaacga
      961 agagatccgt gataaagaac tcagggtcat agacgttgac ggaaatcagc ttggtatttt
     1021 aagtgctgac gaggctctta aaattgcatt tgagaaagat cttgatcttg taaagatcgc
     1081 tcctcaggca actccgcctg tatgccgtat catggattat ggcaagttct gttttgaaca
     1141 gacaaagcgt gcgaaggaag caaagaaaaa tcagaaagtt attgagatca aggaagttag
     1201 aatgtcatct acgatcgaca ctcatgattt taatgtaaag gtcaatcagg ctgttaaatt
     1261 ccttaacgga ggagataaga taaaagtttc tgtaaggttc aagaaaagaa ctttggctca
     1321 tccacagttc ggtgaagaac ttcttgcaaa attcaaagac gctatctctg aagccggcgt
     1381 tgttgacaag ccatcaaaaa tggagggacg cagtcttgtg atgttcgttt ctccgaaagt
     1441 aaacaagtaa aataataagg aggataaata caatgccaaa gatcaaaaca cattctggtg
     1501 ctaagaagcg tttcagtgtt accggtagcg gtaaggttaa gttccagcac acaaacaaaa
     1561 gacacagact tactcagaag gatcacaagc gtaagagaat cctgcgtggt gcagcttatg
     1621 cagatgatac caacatcaag aacgtaaagg ctcttatccc ttacaaataa acgagttgta
     1681 gggaaaacaa attttaaaaa tttcaggagg taataactat ggctcgtgta aagggagcaa
     1741 tgatgactcg taagagaaga aataagactc tgaagcttgc taagggatac tttggtgcca
     1801 agagcaagca cttcaaaatg gctaaacagg ctgtaatgaa gtcaggtcag tatgcgtaca
     1861 ttggaagaaa gcagaagaag agagatttca gaaagctttg gatcacaaga atttcagcag
     1921 ctgctaaaat gaacggtatg aattattcaa cattcatgaa cggcgtaaac aaggctggta
     1981 tcaatcttaa cagaaagatg ctctccgaaa tagctatcag cgatcctgct ggtttcacag
     2041 cgattgcaga aaaggcgaag gctgctcttt aatagtcaaa aacacgcacg ttcttgtaac
     2101 ctgcgtgttt tgttgtatat taaggcaaca ccatatatga cgcatttggc aactaaatga
     2161 ttaaattttc agaataagag gtgacgcagt ttgcatatta atagtaaaga taaccctaga
     2221 gtgaagcttt tcagaaagct tctgtcgtca aaaaaagcta gggaagaaca tggattgttt
     2281 gctgttgaag gtgcaagaaa ctgcgttgac accgcttatg aagctgtgtg cggaagaata
     2341 aaagtccatt ctgttttcta tactcaaacg gccttgacta agtataaaaa tacacttaaa
     2401 actgatagcc ttttctcgtg cgcaagaccc gaagatattt ttgaaatatc tgatgagctt
     2461 gcgaaaaaga tgacagatga gggaaactct cagggcattt atatgatcgt ttttaaggtt
     2521 gatttgcgtt ttttgactga nnnnnnnnnn cggttctgat tttcactcca tcgagcaaac
     2581 gtatatggga agtttctgca aattatgatg atatagttga gttcatgact gcactcgatg
     2641 atcatatggt cagcggactt aatgataaag agctgcagag gataggaaaa tatagtctta
     2701 aagacgcaga aatcataaaa aaacgctgtg aagagcttgg cataaatatt tattgttacg
     2761 aaagtgaggg ctatcctgac agacttaaac ggatcgcaaa tccacctgct gttctgtata
     2821 cttatggtaa ccttgacttt ttaaacgata aatgcgttat ttcagtagta ggctcccgtg
     2881 agccgtctga atattctgta aagataacga agcatatttg cacagatctt ataagaagaa
     2941 actgtctgct tgcttcgggg tttgcaggag gtatagatca gatcgctaac agggtagcta
     3001 ttgaaaataa tagtagacct atagctgtgt gtggcacagc tcttgattgt gattacccta
     3061 agggaagtaa tgagctaaaa aaacagatcg ctgagaatgg agtagtaata tcagagtatt
     3121 ttccgggtta taaaccattt ggaagtgctt ttgtgaatcg caataggatt cttacaggac
     3181 tttcaaatgc agtgcttttt actgagtgta gcaaggaaag tcatggtctt gataatgtca
     3241 atcacgccat atctcaagga aaacaggtat ttgtagtacc gccacatgat atctatgaca
     3301 gtcgatattt cggacaacgt gatctcataa gaaatgagtg tcaacctgtg ttcggcgcag
     3361 aggatatagt gtattcgctt ggcaatgaaa ggtatcaatc tcttagactt gtggaaagcc
     3421 tgggagagtt cacgcttcct gctgaggatt ctgctatact taacaatggc aagcctaaga
     3481 cagtgaagca cagaaaaagc aaaagaaaag tgcctgacag caagactgtt tctgaaacag
     3541 aagtggtaaa gccaacacct gagcctgatc tgtcagggct tgatgacaca caaaagagca
     3601 tttgcgaact gttaaaaagc ggaaatatgc ttgcggacga gataggcgca aagcttggta
     3661 tggatatctc tgatgtgctt gcacagctaa cggagcttga actttatggc actgttaagt
     3721 catatccggg caaaatgttt ggactttaat atatattaag aatggagaat agatttgtct
     3781 aatttgatca tagttgagtc accaacgaag ataaaaacaa ttaagcgtta tcttagcgat
     3841 gattatgaag ttgtggcttc aaagggtcat gtccgtgacc tgccaaaatc aaagctgggc
     3901 gtcgatatag at
//
LOCUS       DAWEMR010000219         3898 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8179_length_3898_cov_11.042675, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000219 DAWEMR010000000
VERSION     DAWEMR010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_3898_cov_11.042675"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1213 a          741 c          920 g         1024 t
ORIGIN      
        1 gaggggtata tagcttatac tttctatctc ataaattccg gtaaagacac acttagctat
       61 gacagcgaaa tgactatcga gaacgtcacg aacggtgttg atgaagctat cagagtggag
      121 cttttcgtga atggcgagaa aactgtttat ggtaaaacaa agtctgacgg cagtggtaag
      181 gaaagcgatt gcgacaagga gttcgcctcc tctactgagg tcatgaagga ccgacgtgaa
      241 aagcttggtc ctggtgagaa agacaagtat acagtagtca tatggcttga gggaaatgac
      301 cctgattgtg ttgacaaaat aatcggtggc acaatgaagc ttggtatgaa tttcaagatc
      361 gttgaaacaa cttaaagaat tagaagggag gaagcctcgg gtttccgagg ttttgaatta
      421 tgaaaaaagc tttgaagatc gtttttaatg ttatcgcatg ggtggtgctt atctttgcac
      481 tgcttataac aattctcgtg ttctcgtctg ataagaataa tggtacggca agtctgctgg
      541 gatatatccc gctcacagtt gaatctgact caatgaagcc aactttcaaa aaaggcgacc
      601 ttattatctc aaaggaaata gacgatatca attctctgaa aaagggcgac gttatcactt
      661 tctggacgct tattgacggc aaaaaggtga agaataccca ccgtatcgca gaggtactca
      721 atgataacgg ctctgtaggc tttatcacac gaggcgatgc taataacgta gatgatacat
      781 acactgttta tgcaggtgat ataatcggtc agtggaatgg tgcaaaaata ggcggtttcg
      841 gcaaggtaat ggattttctc agaacaaaaa cgggattttt catttgcata cttttgccga
      901 tagcactgtt tttcctcttt gagctttata agcttatcgc aaccataatc gagataaagc
      961 gtcctgctat cacagagagc gatgaggaag aaataaaaag acgtgcagtt gaggaatacc
     1021 ttgcacagca gaagaaaaag gaagaggaaa atgcacagca ggagaaaaat gacaaataac
     1081 tgctgcttgg gtgatacatt atgaatggat ttctgaaatg gttttttgtt ttcatgtcgt
     1141 caatgcttgc aggctttggg cagatcttca agggtctgtg gaacggcata aaacagatct
     1201 ttaatataaa aaactacata aggatattca agacctactc ggcagacttt ggtgcgctgg
     1261 gctgggtact ttctgtgctt gcaattatcg ttgttgtggc tatctttgtg cttattattt
     1321 ttatgatagt tcttgctgtg agaaagtaca taagattcag acactctatc gtcagcaacg
     1381 aggatcttat cgaagaaata tctgacctac agcgtcaggt ggtcaaaatg accaaggaaa
     1441 aagacgagat catggcaatg aaggtggctc agcttggcgt atatgataac accgctcttg
     1501 cagagggtga aacaaagctt gcagagggcg agcagatgcc tgcacagggc gaagttcaaa
     1561 ccactgtgga cggcgttgtt cagacagcag attgccgttt ttcaaagctt atcgaggtgg
     1621 acaatttcta caagacatac gagccgcctg cttacaataa tgacatcacc cttagcggta
     1681 tctgcgaagc atacagaaac tttgcctgct caagaatgca cctttattat gacataaaaa
     1741 ctatccgact tttcattgca ggcatggctt caacaaagct cattatcctg caaggtatct
     1801 ctggtacagg taaaacatca ttgccgtatt ctttcggtaa gttcttgcag aaagatacga
     1861 ctatcgcttc tgttcagccg tcatggcgtg acagaactga gcttttcggc tacttcaacg
     1921 agttcacgaa gaactttaat gaaacagaag tcctcaaaag aatttattct tctgaataca
     1981 atgacgatgt aaacttcata ctcctcgatg agatgaacat tgcgagagta gaatattact
     2041 ttgcagaaat gctctcgatt cttgaaatgc ctgatcctgc cgagtgggag cttgatcttg
     2101 ttcctaacgt gtggagtacc gaccctgttc gccttgataa gggcaagctg cgtattccgc
     2161 agaacatttg gtatatcggt actgccaaca acgacgattc cacgttcact atctcagata
     2221 aggtatacga tcgtgcacag cctatcaatc ttgacgcaaa gggagttgcc tttgacgcac
     2281 ctgacacgcc gcctatgaat ttaagctttg aacatattga tacactcttc aaggaagctt
     2341 ttcaaatgta tcctgtttca caggacagtc tgaaaaagat ccagcagctt gacctgtggg
     2401 taattgaaaa gctgagagtg gctttcggta accgtatact taaacagatg aacttgtttg
     2461 ttcctgtata tgttgcctgc ggtggagagg aacttgacgg cattgactat gtgcttgcca
     2521 caaagatatt cagaaaattt gagtcgctta acttggcaat gctaagagaa gagctgaaag
     2581 agctttgcac ctatatgcag aagctttttg gcagaaacac tatgaaagaa tctatagcat
     2641 atcttgaaag attgcaaaaa ctgtactaaa atttgtactc ataaactttg agaggaggga
     2701 cgggcatgag cgaatacaac gaacagcgtg cagcttttga caaagctgta aagacgtttg
     2761 acggcaataa cagctttgcg gcagcccgtg ccttgcttct ttcgggcgaa aaaactgtat
     2821 cttttaatcg taagataatc gagaagaata tagattcgtc ctggctcaca gagatagaaa
     2881 aagctctgcc acatcttgat acggtaataa gaaatcccag aaacaccatt aaagaagtgg
     2941 aagagatcgt gcctatcgct atgtcaagga agataacggt agagtccata aaacatctgg
     3001 gacagcacac cgatcttatt caggatatcg acaaaaagac cggaaagata actccgtcaa
     3061 agatactcaa cattcataaa gaggaaactc ttgatacata tgaaaataga tttgtaaata
     3121 cccttatcga caggctatac atttttataa atataagata caataagctt gtccagaccg
     3181 cagaggcgca ggaggcttat tcttttaatt acgataccgt ggctgacagc ggcgacggca
     3241 gaaaggtcaa cattagtttt aagatagaaa ctgttgacag tcttgtcggc ggcagcaagg
     3301 atactgatgt ttgggcgagg cttgaaaggg tcaaaaaggc tatcgagggc tataaaggtt
     3361 cagtgctttg taccactctt ggcaacgctt ttatcagacc gcctgttatg agaacaaacg
     3421 ctatcactaa aaatgtggac cttatggcgt gtcttaccct ttggtagttt atcgaaagct
     3481 acgataaggc aggctacgag gtcaacgttt ctgatactgc acaaagacct gatgattctt
     3541 atatcgagga tatatatggt ctgaccgcaa tgaactatat gcttttccgc tcctataccc
     3601 atggggcaga taagtcggag gagcttaaaa ccataaagag caagacactc gcaccaaagg
     3661 ttgtcaggcg ttttgataag cagacgtctg ataaatacga tgtcatagtt ggcgatggtg
     3721 aacaaagtag tgcaagtaag acagatatgg gtatgactgc cgaagaggca ggaaacctta
     3781 cggcagagct tgaacgtata gttgagatag aaaagcagtt tctcaaagac gaggaagacc
     3841 gtaagagggc tgaggagctt gcggctgcag aagctgaaag aaagcgtgcc gagcagga
//
LOCUS       DAWEMR010000220         3897 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8183_length_3897_cov_8.455752, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000220 DAWEMR010000000
VERSION     DAWEMR010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8183_length_3897_cov_8.455752"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    641..650
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2183..2192
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          850 a          956 c          839 g         1232 t
ORIGIN      
        1 atcatagtac tccttcttag aaaatggatt atattctaaa tcgttcattt ccaaaccttc
       61 tcctttattg aaatgaattt gattgcatat cgctctcaaa ttataaatca tcgataatat
      121 tattatatca cgaattctgc catgtgtcaa tataaaaatt aagccgccat attttcatac
      181 agcggctttg caaatgtctg attttcggtg aaaatcaccg tttctgtttc ggggcggatt
      241 tggtaatttc ctcggtttgg gcaggagcgt cgcattcctg ctcatcgtca ttgttcataa
      301 tgacgtcctc tgccttgccg acctcaagtt cacggttgag ttgctccagc cttgcggact
      361 tttcggcaag ctcggcagcg tgttcaaacg gcttgccttt agcggctacg gcttcggtaa
      421 ggtcagtctg catacgggcg agtttatccg tcatactgtc gagctttcgt tctaacccct
      481 tactgaacag attttcaagg cgagtgatat ttcctatatc gcccgaaata tccatatcag
      541 tgttataagt cagttccttg cagagagaaa tccctgcgct gacgttaaat ccattgtgaa
      601 acttctcgat tgccagttca aacccgcaga acgttccgat nnnnnnnnnn atcttttcgc
      661 agtgacttat cgtatcgtca aggcggtact gcgcattgta gtgttcgctt tcaagcaggc
      721 gcagacgggc gacatcgccg tcaagctcga ttttctcctt gatacgctcg tcacccgtgg
      781 caagagcctg catttcagag tagttcagaa cagcctcgtc aacgtcctca cagcttcggg
      841 agattgcgtt gccactcata agctgggaga tgaaccgctg ctttgtcacg attatgttca
      901 tgagatatgc gtcgaatgtg ttttcggtga cataattgta cacgccgact tcaccgaaag
      961 tattgccctg acggattatt cggccgtttc gctgctcaat gtctgttgac cccacccaac
     1021 ggaacatctc atagtaacaa aaaattaatc cagtctgccg acgaatttat agtaaacatc
     1081 gagcttctga ataaactcac cgtccacatc ttcaggctcg tgaacctcaa ttctgtctat
     1141 cagagtattg aggagagcct gcgtaagctc gtcaatgtcc gcatactgct tgattaaatc
     1201 aacgaaattt tcggcgttct cgccgttctc acgctccgcc ttaatcaccg aattaagttc
     1261 gcttattctg ctttccaatt cggtctgttc cgtttcatag gcggctgaaa ggctgttgta
     1321 attgcgttcg ttcaccttgc cattgatgtg ttcctcatag agctttgcaa acagcctgtc
     1381 gagttccgaa agccgcttgt tcgctctttt cagttcacgc tcggcttgcc gcacggtgtc
     1441 cttttccgcc ttgcccagcc gttcggcgat tgacgaaatc atctgcctgt catcgcccag
     1501 agcttcattc gccagccgct taatgtccgc taaaaccgca ttgtaaaggt tcagagcagt
     1561 aattctgtgc tgtgtgcaaa ccgacttgcc ctccattcgg tagttgttgc actgatagtc
     1621 gtagtttgca agtatgtttt cgctgttcca atttcggtgc gctcttacat aggtcagcgc
     1681 aaagccgcag tcggcgcatt tcaccaagcc cgaaaagata ttgtcgaact tcttctcaac
     1741 cttgcgagtc tttttcctac tggttatcaa acgctgtaca agctcccatt cttcggggtc
     1801 aataatcgct tcgtgcatac cctccacaac aaatgttcca gccgattgag caggctttct
     1861 cttttcgctt ttcagcgaga ttttagggcg tttctgccca cgaattgcgc ctttgtaaac
     1921 agggttgcgg agtatctcac gcaccgaacc cattgtccat ttgtatgcgc tttcttcggt
     1981 cgtgatatac ctgtcatagc actccaattc ctcggcagcc gcagcaacgg gacgaggaat
     2041 tttctctttg gtgagccgcc ttgcgatttg cgttatgccg ataccgttct ttgccagctt
     2101 gtaaatgtac ctcacaatag gcgcaaagcg ttcatcaatc agcaagtgat ttttgtccgc
     2161 agggtctttc acatagccgt agnnnnnnnn nngggagagg gcacatcttc cagattaagc
     2221 gttctggcaa tttccgtggt gcaaaagccc gccagggtca tatcgaaaat acgccggacc
     2281 actttggccg ctggcgggtc gatcaccagt ctatgcttat cttcactgct tttcacatag
     2341 ccgtagggag cttttgtgcc gatgaattta ccctgcttct gccgcgtcag cagcgaggac
     2401 ttgattttct ttgaaatgtc ctgtgcgtac atatcgttga gcagattttt gaacggcgta
     2461 atgtccattg tagagccttt tgcggtgtcc acgccgtccg ttacggctat gtaccgcacg
     2521 ttgtgttcgg ggaaataaat ctccgtaaaa ctgcccgttt caagataatt tctgccaagc
     2581 cgggaaaggt cttttgtgat aacgcagttg atttttccgt cctcgatatc cgacagcata
     2641 cgctgaaagc tcggtcggtt ggtgttcgtt ccgctccagc cgtcgtcaat gtagatctgg
     2701 ctgattgcaa agccgttttc acggcagtag cgtgtgagca tttccttttg cgtctgtatg
     2761 ctcgagctct cgctcatggt gccgtcgtcc tttgaaagac gaagatataa agctgtattg
     2821 tacttgggtt gtcctgtcat tgtaaacctc cttcaaacac ggacaaccga tttattcgac
     2881 atctttatta tacatcagct gtcccaatat ttcaagtatt ttacggtaca ttattgtcgc
     2941 ttgccgtatc attatttttc ttttcagatt tgttgagttt cttctcaatc tgttcggcta
     3001 ttacctttct gaaaacctcg gtagttttca cagagccgct gtaatgcggc acgacaacga
     3061 ttttgagatt tttctttttg tatcgcataa tttacctccg ttatcttctg ttgtttttct
     3121 tgtttgtggt ttgtgttggt ttaggctgtt cggggaagtt ttcttttttc tgttctgctt
     3181 ctctgcgctc ccgttcggca cggataaggt tatctatata attgccctca acgatttcct
     3241 cgtaagtacg tatcacattc tcaatttgtg aaagctgtgt tttcaagtca atatcatcag
     3301 cgttgttctc ggctttttct cgcaagtcgg cagctatctc gtaaagctgt tcacgggatt
     3361 taatctccaa ctcggacagc gtgttcaggc aatacagcaa acgcccaatc gtcaggtcgt
     3421 tcatgatatc atacggttgc tgcttattcc actttgcagg tgtaatctca aactttctca
     3481 caagcgtaat acttgtgttt atcgaagcgt agaacggctt ttcaagggcg tttgcctgca
     3541 tttcagcttg cctaaactcg tacggaattt tatttttgtt ttcaattcgg ttcttcagtg
     3601 aatactcgga ataatcctcg ccaagcgatt tcagacggaa aaatcttttt gcatagggcg
     3661 gttttatcgc gatgtattta cctctccgca cttcatattt gcagtcctcc agcagtttga
     3721 tgaaatcctc gaaatctctt gcggaataca gtattcggtc gataaagaat ttcagccgtt
     3781 cgcggatatt ccgaccctcc ataggcttga aagcagcaag cgcttttttc acagacggct
     3841 cgtaatattc gggaacatag atcgtgctgt tttcttcgac gaacgggcag ctgacgt
//
LOCUS       DAWEMR010000221         3892 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8199_length_3892_cov_8.388324, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000221 DAWEMR010000000
VERSION     DAWEMR010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8199_length_3892_cov_8.388324"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1210 a          886 c          735 g         1061 t
ORIGIN      
        1 cctacatatt gccccgacta ctcaatacta agaaactcat caggtatgta agacaagcat
       61 tgcttcacgc tctcacgagt gaagccctct acataattct tggcaaacag ctcgtcagtg
      121 tattccgcat aaggataaac agtgcagttg taccaatcgc catagtatga atcaccctca
      181 aagtgcgata ttatcggtat cgccttaacg ttctcgaatg tcacctcatt ggtgtcaggg
      241 tccttcacaa cattgagctt gccaataagt cctacaagcg actttgtgtc atacatagtg
      301 gaaataaagt tgcccaaacc gtagtaaacg aatgcctgtc cgccctcagg cttgtcaaga
      361 tatccgcagg tgctgatagt gtgcgcctgc gtgcctatta tcaggtcagc accccactcc
      421 acaagcttag gcgttatctc cgtctgctgt tcgttaagct cgttctgtat ctctgtgccg
      481 taatgacatg aaaccacaac aacgtccgcc attttgtcag cctcttctat ctgagccttt
      541 atcttgtcct cttcatcaag atatatcacc ttagcgccgt caccgctgag ataattgccg
      601 ttggtgtgtt ccatgtatcc cagaaaagca aattttatgc cgttgacttc catagtcttg
      661 atagtgtcag cgtcctcctg cgaacgataa gcaccgtaat gcacaatgcc ctttgtgtcc
      721 cagtaatcaa gagagcttat aagcccctct tcaccctggt cgagaacatg attgtttgac
      781 atagaaacca cattgaagcc catgtcaact attttgtcgc ccacagcagt tggcgatgcg
      841 aacataggat agctttgtgg ttcaacgctg tctgtaacaa gcgtttcctg attgattata
      901 gccacatcag tgccttctat gtactttgta acgtgttcat aagcctgcgt gaaatcatat
      961 ttttcaccac cgccaagctt tagcgcctca ttataaatgt tgtcgtgtat aaggttgtcg
     1021 cccacgcaaa ccagcgagca ggtgtagctt ggctgttcct tttcttcttc attcacctcg
     1081 acctcttttt tgctggagct gtcctcagtt tttttgactt ccgctttgct ctcggtttcg
     1141 gtcactgtca aaacgtttcc gtcaagcttg ctcgcgtcaa gcttaccgca tgatacagca
     1201 ctcagcgaca acatcgccga aagagccact actgtgatct tattgtataa aagtctgtac
     1261 ttcatataaa ttcctctcat aatatcttaa aaaaaataca aatttactta cagtttaatt
     1321 ttaacacatt tttaaataat aatcaagtca aattttcaaa atgtcgaatg ctttatgttg
     1381 acctatatat tataatgtat tataataaaa atataacaat ttgtacaaat atttgtttat
     1441 gtgtacaaaa attttcccca caagtattaa attgtatgct ataataaata cacagataga
     1501 tttgtctatc taaaaaagga gaaatgacca tggaatatta cgcacacacc cgtgaggacg
     1561 gtgaaaggca aactgtaaaa gcccacctta taggcgtaag caaattggct gaggactttt
     1621 cagtagatct tttgaaaccc cttgcgaaaa aagcgagcct gtatcacgat ataggtaagt
     1681 atgctcttaa ctttcagaaa cggcttgatg atgacaaagt aaaattcagc cacgctgcct
     1741 gcggagctat agagtataaa atacttgcag ataaaaacga ttgttttgca ccccttatgg
     1801 agtattgcat cgcaggacac cacacaggac ttatggacgg cggcacaggc gcagacaact
     1861 ctgacagccc gaccttaaac ggtacactaa aaagagcaga cgaatataca ggcgatagtg
     1921 actacagcgc atacgccact gagatagact ttgccacact gactcaggaa gaaagaacgc
     1981 cgctgcgtaa tgaactgctt tctacaaaag accccaccga gcttatcgag cgttacgcct
     2041 ttttcaccaa gtacgtgttc tcttgcctga cagacgcaga ttttctcgat accgaaatat
     2101 tttgcaataa aaatgttgaa cgtggcatga gcggtgattt tgaaaaggca cttgataaac
     2161 tgaacagaga attaagtgat atgccgtcag ataacccgtt aaggcaagca agaagccgta
     2221 tccagcaaca agcttttgat aattcggtca ataaaagtca tatttcaatt cttgatatgc
     2281 caacaggtag cggaaaaacc ttatgtagtt tgaaactagc cttagagagc ggaaagaaga
     2341 gaatcatata cgtcatacct tacacaagta taatagaaca gaccgctaat aagtttgaaa
     2401 aaatgttcgg cgacgttctc cctgtcctac agcaccattc aaactattcc tatgacggcg
     2461 acacagagga agaaaagaaa accgcagaga agctcaaaag gacctgcgaa aattgggacg
     2521 caccacttat aatcaccaca agcgtgcaat ttttccaatc catataccat tataaaggtt
     2581 cagcactgcg aaagctccac aatctccgtg acagcgtgat agtttttgat gaaatacacc
     2641 ttattccaac agaacttctt cgcccctgcc ttaaagctgt gggctatata acaaagtatc
     2701 ttaacagcga agcccttttt ctctccgcca caatgcccga ttattctaag ctttttgata
     2761 agttcctgcc tgatgtgaac tataataagc ttgttaccga ccgaaccaat tttaagtatt
     2821 tcaagaaatg cgaatataaa gacatgggca aaacctctct tgaaaccatt gcagaaaatg
     2881 cctctcggtg taaaaatgcc ctgatcgttg tgaataccaa gaaaacatcg gcagagcttt
     2941 atagccttgt tcaaggtgaa aaatatcacc tttcagccaa tatgacccca gcccacagaa
     3001 gccgtgtcat agaagtagta agaaataagc ttgaaaatgg agaacatatc acagtagttt
     3061 caacttcact tgtagaagca ggcgttgacc ttgactttaa cacagttttc cgccagcttt
     3121 caggtttgga cagtattttg caggcagggg gcagatgtaa tcgtgagggt aaagatgcca
     3181 agggctatgt gtacgttttt gacattgacg aaacataccg caagggaagc gaccttgcca
     3241 tgcgaattaa caagactaaa ggtttgctgg aaaagtacca agacataact tcctatgatt
     3301 gtattaaaga atactacgac ggcattttcg acttcaacca aagtcgtata gctgaaaact
     3361 ctattgcaaa atataacgaa caaagcaact cattcgaccg ccaaggctta atgttaccat
     3421 acagcatacc attccgaagc tatgcaatgc agtttgaata catctccgcc gacactatct
     3481 ctatcgttat cgacgatcct aacgaccaga cttgccatga acttgttgaa accttgcgta
     3541 agggtgatat gtcagtaaga agagcattgc agaaatattc agtgtccgtc tatatgaacg
     3601 ttttcaagga tctctattct cagggtgttc tcaaagatca cggtacaggt atattcattc
     3661 ttgaaaatca aagctattat aataatgaaa caggcttgaa cacccaagcc gcaatgcagg
     3721 attatttcat ctaaaacaaa tggcaggctg ccaccaagac agtctgccac ttgccgtgtt
     3781 ccgtaccaca tagaatacaa atatattaca actcacatat gcgtaacgca tagacaaata
     3841 cattatacaa atataagctt aaaaagtcaa tgatttatgc cacaaactgt tt
//
LOCUS       DAWEMR010000222         3886 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8213_length_3886_cov_11.304620, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000222 DAWEMR010000000
VERSION     DAWEMR010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8213_length_3886_cov_11.304620"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          996 a          903 c          777 g         1210 t
ORIGIN      
        1 caaggttgct gcattatctt cggctcttat cgtacacgaa gtcgcaggaa tgtcagaggg
       61 gcaaggcaat gccacagcga attatgaata ttacagagaa tgtttcggcg aaacgtctga
      121 ggtgctgaac agcaggaaca atcccgaatt tcttatagac gaacttcagc gagagggcaa
      181 aaatatccca gatatattta tgtgctgcgg tacagaagat ttcctgcttg aaaacaaccg
      241 tgatcttcac aaatttcttg aaagcagaaa cgtttctcac gagtattttg aaagcaaagg
      301 caaccacgat atgatttttt ggagtgagta tgctgaaaag gcggtcaagt ggatgtttga
      361 ggagaagtaa gagcggtgat gtctttgcgg tttttatgtg ataatatgaa acacaagaaa
      421 cctcatacaa actgcacaat ataaaaatac cctttgagaa agggatttct cctctctcaa
      481 agggcaggtt ggcaaactgg aatttatctg tctctttctt tatttttatc agcatttgct
      541 cttcttttta ttcttaatat taaactatca ggacttttct tttcttccgt atttttgcct
      601 gcattagctc ttgcttttat tcttgatatc aaattatttg tattttcttt tttattcttt
      661 tccatataat taacctccct acaaattccg atttgtattt ctgtttgttt taaaaatggg
      721 caaccccgat tggaattgcc cattaattct acaaattatt atgttatggt tactccaacg
      781 tcttcatcgt agggaacaac agcacatctc tgatagccgg tgagttagtc agcagcataa
      841 caagtctgtc tatgccgtaa ccaataccgc ctgtaggtgg catacctatc tccaaagccc
      901 tcaggaagtc ctcgtctgtg tggtttgctt cctcgtcacc ctgcttcaat gcctcttcct
      961 gcgctgcaaa tctctcacgc tgatctatcg gatcgttcag ctctgagtat gcgttgcaca
     1021 tctctctgcc tgtgatgaaa agctcgaatc tctctacata atcaggattt tctggcttct
     1081 tctttgtaag tggagaaatc tcgatagggt gatccattat gaatgtaggc tgaataagat
     1141 gctcctcaac atattcctca aagaacaggt tgagtatgtc gcccctcttg tgccttgcct
     1201 cgtattcaat gtgatgctcg tctgcaagtg cctttgcttc ctctgttgtg gctacctgtg
     1261 agaaatcaac accagaatac ttctttactg cgtcgatcat tgtcattctc tcaaaaggct
     1321 taccaagatc tatcattaca tcaccatatg gtacacaagt tgtgccgcat acctcctgtg
     1381 caacgtgcct gaacatattc tccgtcagat ccatcatgcc gtagtaatct gtgtatgcct
     1441 gataaagctc cataagagtg aactcaggat tgtgtcttgc gtcaacaccc tcgtttctga
     1501 atactctgcc tatctcgtat actctttcaa gtccgccgac aataagtctt ttcagataaa
     1561 gctccaaaga aattctaagc ttaacgtcct cgtcaagtgc gttgtagtgt gtctcaaatg
     1621 gtcttgcggc agctccgcct gcgttagata ccagcattgg agtttcaact tccatgaagc
     1681 cctgtccgtc aagatatctt ctgatagatg aaattatctg cgacctcttt ataaatgtat
     1741 cctttacctc aggattgcat ataaggtctg tgtatctctg tctgtatcta gtgtcctgat
     1801 ccttcagacc atgccacttc tcaggcaacg gcagaagcga ctttgaaagc agtacgaact
     1861 tctgagcgtg aattgatatc tcgcccattt ttgttctgaa tacaaagccc tcaatgccaa
     1921 cgatatcacc gatatcgccc ttcttgtaat cgtcaaattc ttccttgccg atctcgttca
     1981 ttctcagata gacctgaacc ttgtcgctct tatctctcag gtcgaaaaag cttgctttgc
     2041 ccataagacg tcttgacatt actctgcctg caacgcttac tgttatgcgg tttgcctcga
     2101 gaagctcctt gagcttttct tcgtcctcgc ctgcctgctc cttaagctca gcctcaagct
     2161 tttcatactg tgctttagcc tcggcacagc ttgccgtaac gtcatacttt gttatctcaa
     2221 atggattctt gcccttggct ttcagctcct ccagcttgtc cattcttatc ttctttaagc
     2281 tgtttatgtc ctcctgagta agctcttcct caggagcgtt tgtgcggttt tcctcgctca
     2341 tttatatttt cctttccgtg tgttttgtaa gtggtctgtt actttgaaat gtcgatgacc
     2401 tcgaatttca gcagaccggc aggtgtttca gcctcgaaaa tgtcgcctac cttgtgcttg
     2461 agtgcagcct tgcctatagg tgactcgtca gagatcttgc cgttgtcagg gtcagactca
     2521 gttgaaccta ctatctgaag atcctcgatc tcgtcaagct cgatatcctt tatcttgatg
     2581 atagagccta cgcctatctc gtttacagaa atatcatcgt cctcaacgac agttgcgttt
     2641 gaaatgagaa gctctaactc attgatcttt gattcaagga tagcctgctc gttcttagcc
     2701 tcgtcgtact cagcattctc ggaaaggtct ccgaaagaac gtgcttcttt aagcttctga
     2761 gcgacttcac gtctttttac tgtaatgagg tattcaagtt cctcctgcaa tttagcataa
     2821 cctgcttttg aaacagtctt agaaatagct gccataatta aacactccta tatcaatata
     2881 attttatacg ggtactatca acttaatatt atacacgatt tgccctcttt tgtcaaggtc
     2941 ggaagccgtc gcaaacgggc ttttttgcac ctttggacag tctgcacgat tttacatttt
     3001 gcacaaattt tcacctctga cagcgtgtta ggatattgaa aataataata gtatgatcta
     3061 taattatctt atgataaaac aagttatgaa aaaacatagg aggaaatttt catgccaaat
     3121 cccattttgc ctgcatggga gtatatccct gacggtgagc cgagagtttt cggcgacaga
     3181 gtttatcttt atggctcaca tgacagagcc gctcaaagcg acttttgcga ctataaactg
     3241 aaagtctggt ctgcaccact tgatgacctt aacaattggg tctgccacgg acacagcttc
     3301 catacccgtg atgaccgtga ccataagtcc gataccgagt taatgaccga ccgcaagctg
     3361 ttcgctccgg acgttataga aaaagacggc aagtattatc tctatgccta tatagtcggc
     3421 tcaaagggag ttgttggcgt gtccgataag cccgaaggtc ctttcaagct cctttctcag
     3481 tataaatatg atcctgagga cgcaggcgat gaaggcattt ataatgatgc aggcgtgctt
     3541 gttgatgatg acggcagagt gtatatttat tacggcttca cagaatccaa cttcaacgag
     3601 atagaccctg cggatatgta taccattaaa aagggaagct acaaaagagc ggtcattgac
     3661 gacagcgaca acgcaccgca ggaacagcgc ttcttcgagg caagctcccc gaggaaaatc
     3721 ggagatacat actatcttat atattcccca tgtgtaggaa gcaggcttgc ctatgcgacc
     3781 tcagataagc cacaaggtcc gttcaaatat cgtggatata taatagacaa cggcgtggat
     3841 ttcccgggtg gaaatgacca tggctcagtg tgcaacataa atggtc
//
LOCUS       DAWEMR010000223         3881 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8230_length_3881_cov_15.146628, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000223 DAWEMR010000000
VERSION     DAWEMR010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8230_length_3881_cov_15.146628"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1279..1378
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          970 a          785 c          843 g         1183 t
ORIGIN      
        1 agcgggaaca aaaaaatccg ccctacccaa agtaagacgg aaattgggtg cagggtcgaa
       61 actttcagaa aaagaccaac gattggtgct tgaagtttct gaaagactag gaaaagaata
      121 atttttgagg tctcaaactg caatatcaaa gtaaaaaaac agcgtaccgc agccccctgc
      181 gatacgctgt tctcatataa aattacttct ttgtagtcac actctttgaa gccgaccaag
      241 caccataata cttagtaccc tttacagttg tgtaagaacg aacacgaacg taatatttct
      301 ttttgcctga aagctttgaa acagtggcct tgtcagtctt gttgtttgtt atggtaactt
      361 tctttgcact tgtaaactta gagtttgtag cgtactgtat ctcgtatcca gtagcagaac
      421 ctttctgcgc ccaatccaca aagaatgcct tgctcttagc tgtgagcttc tgtatctcct
      481 gcttggcagg atttatcttg aatgtctttg tgatagtgcc tgtataagag ccttttcctg
      541 cgatttttac tgtggcagtg cctatgctct tgttgcttga gtaggaaact gtatagtcag
      601 tgccattttt cagcgttttg ccattgtatt tagcagtaat gctctgagta atatttttgc
      661 ccgtgtatga cttgtttgaa atgcccgaaa ctgtggcttt cttgaagtta tttttgatac
      721 tgtaggtctt agaaactgag cctgtgtagt tgctcttgcc cgtgattttt acagtagctg
      781 tgcctacctt ggtgttgttt gaataggaaa ctgtgtaatc tgtaccattc ttcaaagttt
      841 tgccgtttag cttgaccgtt acaccaggct tctttgcctt gccatcatat gcgtaggtcg
      901 aagtggaaag tgtcacgctt gcctttgaaa tgctaattct tgaaagtgcc gggatctcag
      961 ccgtttcaag aactttcttg catactgtac attccttgtg ctttgagcct ttaactccga
     1021 tagaagccgc cttgtcgact atccaaccgc ttgacttgtg acctgtggca tttatcactg
     1081 tctgagcctt gataacagta ttgcaaactg agcaatgtga gccgtctgtt ttgcctgcgg
     1141 tggtgcaagt agcagggtag cccttgtcgg taactgcagt gtgacccttt gcaggaagct
     1201 tttctgtaac tgtcgcattg cattttgtgc aagtctttat agcagtaccc tctgatgtgc
     1261 aggtcggctg ttttgttann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     1381 aagtctttgg tatagtttct gtaactgtcg cattgcactt tgtgcaagtc tttatagcag
     1441 taccctctga tgtgcaggtc ggctgttttg ttacaacaga atttccgtag ctgtgacctg
     1501 ttgcctttgt tgtgctgtcc ttgtatgaag taccgcaaac agaacacttg tggagtgtgt
     1561 agccgtcagt agtgcaagta ggtgcaacaa ctgtgtcagc atatttgtgt gaagtctttg
     1621 gtatagtttc tgtaactgtc gcattgcact ttgtgcaagt ctttgttttt gtaccctctg
     1681 atgtgcaggt cggctgtttt gtaataacag cattgccata tgtgtggctc gtgcatccgc
     1741 aggtgaattt gtatgtcttt gctacagacg gattgtatgt aggataaatt tttacagtga
     1801 gtgaaccgcc gtttttgaat gtgatacgtc tgatatcatt agcatagttt tcaagcttat
     1861 ttacacttac catactgcga tcagaaaatt caactgtgta gtctgtatcg tcataaagcc
     1921 agaaatcaat gctgtcgccc acactgaact gagttttgct caatacgctt gaaaaggacg
     1981 tattcgaaag gtctgtgcgc caataaacag tggtagaggt cggaactgtg aacttattca
     2041 cataaccgca agacttgcag gtctgtgtta cagtgccgtc agtttttgat gcgtactttg
     2101 tttcgtagtc atgacctgtt ttgacgtcaa ccgtctttat atcgtcaaga tttgaaaggt
     2161 tcagggagtt gaaggtaacg ttatttttat cgtaaacata ccaaactgct ctgccgtttt
     2221 tgataacagg ctggcaatct gaaaggcttc cctcaaaggt gtgtatactg ccgtttactg
     2281 tgccgtcagc gtttagcttc acacagctta cctttgtatc tctggcccac aatagcaaaa
     2341 agctgttatt atttatcttc acaagctgtg gagcagaagc tgagtctgta ccctctgcat
     2401 aagaagttat cttattgagc ttgtttgtgg aaaggtcctt tgaaacagag gaaacgtaga
     2461 cgtttcgtgt ttctgacgta ttgatataat caaggtcaac tgtactctgt gccacgatat
     2521 agcttgatga tgacacatca aagccgccta tagccgcacc tgtatagtta tagtgaccgg
     2581 cggtatattc agggtatgtt acaacgtcga tattgctgac ctgttcaaaa tagcttggga
     2641 aaaatttgcc tgtagtaaaa tcagaattat acttcaccag aacggcggaa cgtggatgag
     2701 cgtcaccatg gtcgagggcg actatatggt tgccgtcggt ttttataaac tgattgaagg
     2761 agtggctaac atagccataa tcaacgttca tgacgccggt atatgaatca gtgatagtca
     2821 tcgaaggcat atccacttca atggtaacat tagcctgatg attattgccg tcgcttgatt
     2881 tatacatttc gtggcaggtc ctcacaagca ggtggtcacc gctatgggtc attcttgccg
     2941 agcctgcatc gaatggaact gtagtgttag ctccatacag accgcaggac tttattttgt
     3001 tccaattctt atcatacttc gtgatacgga aaacctcgag ggagtcgttt tgtttcggat
     3061 tttcctgacc gctaagtaca taataattat tgccggagtc atagaaagca ccaaagatcg
     3121 gcagttcatt gtcgataagc ttagtgctga gcggttcaaa atcagagctg taatattcca
     3181 caaggagctt gcccttgata gcgcctgact ggacacgcat ataattgccg ttgtcgcaca
     3241 ctgttaggta agatttcact gtgtcagacc attgcacata gtcctgatca ttcacattag
     3301 agcctgaata cgcaacacat tgcgccacgg caaaggcact gaacgatcca gcagacacag
     3361 cagtagaaac agccattgcg ccagacagga caatgctcaa cattcttttc ttcatattca
     3421 ttaatttcat cacctcatac aaaaactttt aaatgagaac acctttatat atataatatc
     3481 acaacgcaag gcatatgtca atgaaaataa gttacaatga gaatgatttc aataaattcg
     3541 tcatacacca aaggtcaacg cagtaaaaaa gagcaaagag gatacaaaac agaaaaaaac
     3601 gccttgacaa ggtttgatgg gtgtgatata atattacagt ggtatttcga ggtgtggctc
     3661 agtttggtag agcgctgcgt tcgggacgca gaggccgtgg gttcaagtcc cgtcacctcg
     3721 accagtcact cgccgtgacg ggcatcgtcc gtcatggctt tttttatcga cgaaattttg
     3781 caccgctcaa tacactttat tattgccttt acaaagttgg gcgtgcccct gcacccaatt
     3841 tccgtcttac tttgggtagg gcggattttt ttgttcccgc t
//
LOCUS       DAWEMR010000224         3859 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8273_length_3859_cov_12.085962, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000224 DAWEMR010000000
VERSION     DAWEMR010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8273_length_3859_cov_12.085962"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          961 a          951 c          769 g         1178 t
ORIGIN      
        1 gcaccagtga tcatgttctt tacatagtca gcatggcctg ggcagtcaac gtgagcatag
       61 tgacggttct ttgtctcata ctcaacgtga gctgtgttga ttgtaattcc tctctctctc
      121 tcctctggag ctgagtcgat attgttgtaa tccttcttct cagcaagacc ttcaagagca
      181 agagtctttg tgatagcagc agtaagagtt gtcttaccgt ggtcaacgtg accgattgta
      241 ccaatgttaa cgtgtggttt ggttctttcg aaatgtgcct ttgccatagg aattttcctc
      301 cttaaaattt aatagtttcc gatttaacat accgtagtat aattatatca aataaaaagc
      361 aaatttgcaa tagaaaaagc agaaaatttt ccgcaaattt attagtcctt gtttcttgaa
      421 gccatgatct tctcagagat agactttggt acctgaatgt agctgtcagg ctccattgtg
      481 tactgtccac gaccctgtgt cttagaacgc aggtcatttg agtaaccaaa catttcagac
      541 agtggaacga gagcgtctac ctgaacagca ccagttctct gctcctgacc gaggatctgt
      601 ccacgacggg agttgaggtc gcctataact gttccgagat aatcatcagg agcgataacg
      661 caaaccttca tgataggctc aagaatgatt ggtgaagcct ttctcatagc ctccttgaac
      721 gccatagaac cggcgatctt aaatgccatt tcagaagagt cgacttcgtg ataagatcca
      781 tcgtacagct caaccttaac gtcaactacc tggaagccag cgagaacacc gttctccata
      841 gcgcccctga tacctgcgtc aactgcagga atgtattcct taggaatagc accgccgaca
      901 accttgttga tgaactcata gcccttaccg gactcgtttg gctcaacgtt gatcttaacg
      961 tgaccgtact gacccttacc acctgactgc ttagcgtact tgtaatcaac gcttgcagga
     1021 gcagtaattg tttccttata tgaaacctga ggagcaccga cattagcctc taccttgaac
     1081 tcacggagaa gtctgtctac gatgatctca aggtgcagct cgcccattcc ggcgataatt
     1141 gtctgacctg tttcctcaga agtataagtt ctgaatgttg ggtcctcttc agcaagcttt
     1201 gcaagagcga tacccatctt ttcctgacct gccttagtct taggctcgat agcgagctgg
     1261 ataacaggct cagggaattc cattgactca agaagtacag gatgctcttc atcacaaaga
     1321 gtgtcacctg ttgttgtgtt cttcagaccg atagcggcac cgatatcacc agcatagata
     1381 gtatcgatat cctttctagc gttagagtgc atctgaacga ttcttccgat tctctcgtcc
     1441 ttgtccttag tggagttata aactgtagaa ccggctgtca gaacacctga ataaactctg
     1501 aagtagcaga gcttaccaac gaatgggtct gtagcgatct tgaatgcgag agcagcgaat
     1561 ggctgatcgt cgcctgaaat tctctcaact tcctcacctg tatttacgtc agtacccttg
     1621 atgtgtggta cgtcgatagg tgaaggcata taatctacaa tagcgtcaag aagcttctga
     1681 acgcccttgt ttctgtatga agttccgcag gtaacaggta ccattgtgtt agcgatagta
     1741 ctctttctga ttaccttctt catctcgtcg atagtgatct cttcgtctgc gaaatacttc
     1801 tcagcgagat cgtcatcaac ttctgcaaga tgctcgataa ggtcagcgtg atactgttca
     1861 gccttttcct taaggtcctc aggaatctct tcctgacgga tatcctttcc aaggtcatca
     1921 tagtatacat aagccttcat ttcgagcaga tcgacgatac ccttgaagtc gtcctcagca
     1981 ccgataggga gctggatagg aacggcgtta caatgaagtc tttcatggag catattcaaa
     2041 acgttgtaga agtttgcacc catgatatcc atcttgttga catataccat tcttggtacc
     2101 tgatagttat cagcctgtct ccaaactgtt tcagtctgag gctcaacacc acccttagca
     2161 caaagaactg ttacagaacc gtcgagtaca cgaagtgaac gctcaacctc aacagtaaag
     2221 tcaacgtggc ccggagtatc tataatgtta agtctgtgac ccttccagta gcaagtagta
     2281 gcagcagaag taattgtgat acctctctcc ttctcctgtg ccatccagtc cattgtagat
     2341 gcaccgtcgt gtgtttcacc gatcttatag tttacaccgg tataaaacag gatacgctct
     2401 gtagtagtag tcttgccggc gtcgatgtgt gccatgatac cgatattacg gtaatcctgc
     2461 aatttacatt ctcttgccat atatatcctc ctttatacct cttaccatct gtaatgagcg
     2521 aaagccttgt tagcttcagc catcttatga gtttcgtcac gcttcttgca tgaaccgcca
     2581 acgccgttaa gagcgtcgag gatctctgct gcgagtctct ctctcattgt atcctcgttt
     2641 ctctcacgag aatactttgt tatccatctc agaccaagtg tctgtcttct gtcaggacga
     2701 acctccattg gtacctggta agtcgcaccg cctacacggc gagccttgac ctccagctca
     2761 ggcatgatgt tatccatagc ctcctggaat acttcaagag caggtctgcc tgtcttttct
     2821 tcgacaattt caaatgctcc gtaaacgatc ttctgggcaa cacccttctt accgtcaagc
     2881 atgatgttgt ttacaagacg tgtaacaagc ttagaattgt atactggatc ctgaagcacg
     2941 tctctctttg taactctacc tcttcttggc actttaattc cctccttcac aaataatgaa
     3001 ttcacaggta ctcgctctgt aaccacggca catagccgtt ttacagcccc gtcagttcct
     3061 tgtagagtca actaatatat attaatcact ccacataaac tgtggtcttc gcaatatgcg
     3121 acaatgagtt attaccttgt tactaaaatg agcttttcag tttttgatta cttggctgct
     3181 cctgcctttg gcttcttagc gccatacttg gatctcgcct gcattctctt agcaacgccc
     3241 tgagcgtcga gagtgcctct aatgatgtga tatcttacac cagggaggtc cttaacacgt
     3301 ccgcccctga taagaacaac ggagtgctcc tgaagattgt gtccgatacc aggaatgtaa
     3361 gctgtaactt cgtttccgtt tgtgagctta actctggcaa cctttctcat agctgagttt
     3421 ggctttttag gtgtagcagt tcttactact gtgcaaacgc cacgcttctg tgggcagctc
     3481 tgatcgataa ctaccttctt ctttgagttg aaacccttct gaagagcagg agctgtagac
     3541 ttttctgcaa ggacatttct ttcttttctt actaactggt taaaggttgg catattttct
     3601 ctcctttcat agatttgata ctatacgctg atatacaata catcagcata ccttgatatt
     3661 atacactatt ctccgtctct tgtcaagcac tttttcttga cttttttaac tttttgaaaa
     3721 tttcagttaa attttctgtc aaaagctata aaaagcacag ggcagcggct tttcatgccc
     3781 tgccctgcaa attattcctc attccgtctt gctgtctgta tctaaaattt ctcctttatc
     3841 cgccacttcg acctctgtc
//
LOCUS       DAWEMR010000225         3830 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8332_length_3830_cov_11.220132, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000225 DAWEMR010000000
VERSION     DAWEMR010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8332_length_3830_cov_11.220132"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    315..324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          962 a          879 c          669 g         1310 t
ORIGIN      
        1 gtaggggctg gcgccctcga cagcccgaca tcgaagcggc tagacttgga ttggttacga
       61 atgaaaagaa gcagacaatg ttataaattt ttgcaccgaa cattttgtaa aaaaatacaa
      121 atgatattga aaccgaaaca tcgataagcg ggacgtcggg ggcgccgtcc cctacaataa
      181 aaacaagcaa aatagcaaaa aaacacgctg aatttttgtt cagcgtgttt tcttattgtc
      241 atttttaatt tagaactcta tctttgagta aagacctgaa tccttcattg tgtcctctgg
      301 ctttggcttc ttgtnnnnnn nnnncctctg ttgtcatact ttctaggtgt tgtagagctg
      361 attattacct ttctccatgg ctcttcaccc tttgactgag atgttacacc ctcgatctct
      421 gatacagcag cgtgaatgat tcttctctca tatggattca ttggctcgag agctgatgaa
      481 cgaccgcttc tctttacgtt gtttgctatc tttcttgcaa gctcttcaag ctgggtcttt
      541 cttcttgccc tgaaaccatt gcaatctgtg gatattctga aatattctct gtctatcctg
      601 ttgcatacaa gtgatgcaag atactgaagt gaatcgagga tatcgccctt cttgccgata
      661 agcttctcaa actcttcgct ctttatctca aaaagtgcgc cgttttcaag ctttgttacc
      721 tcgatttctg tgttttctat gcccattgcc tttataacat tgagtatata ttcctttgca
      781 cagtcagcct ttttctgcaa agtgtcgctc cactctgcct ctgccttgac ttcttcctga
      841 acttctgcct tttcttcaac agcctctgtt ttttcagtta cagtttctac ctttgtttca
      901 ggctcatatt cagcttctac ctttgcctca cccttgatct ttccgaaaaa gcccttttta
      961 ggctcttcaa gaactgtgaa agtgatcttt tctacttctg ttccaaattc aactgccgcc
     1021 ttgttttttg cttcgtctac agttgcggct gtaaagatct gtttcatttt ttctgctcct
     1081 ctcgctaaaa aagtttagtc aacaacaggt caatcctgtg aatcgtcata ttcgtcgcca
     1141 tatttctcag ccattctctt tctggcttcg ttaagacgtc ttctctgctc gtctttaagc
     1201 tccgtctttg ttttcttctt aggctcatcg taatcctcat cgtcagaaag tgactttctg
     1261 atctcagcag gatccttacc agcctgtatc atctgagcct cagccatttt ctccataaga
     1321 gtaggcttgc ccttcttttt ctgctttgct atatccttct gaacaagctc tgcaacgtgt
     1381 tcaggtgtgt agatcttatt gagtacgata gtctgtccaa gtgcaaacag tgaagaatag
     1441 caccagtaaa gaccaagacc tgccggatag ctgaaaccga tccacagtga gaagataggc
     1501 ataccataaa gcattatctt cattcccatt ccgcccatat ttgcagcgtc aggattgttc
     1561 ttttttgcaa aatgctgtga aacgaatgtc actacaagct gcaaaagtgc tgatacaata
     1621 gggataatgc aggaaagatc cttcattgtt ggtatctttc caaggaaaag tccgaaaatg
     1681 ctgtagttga aatcttcagc cgcagccttt acgcttgttg gaagcacatc tgcatactgt
     1741 ccgccctctt tttcagtgaa aacgaaagtc ataagctcag gtcttgactg aatgaggttt
     1801 gtggagaagt aattctcgtt aagaataaaa gtatctatat cattatgagc cttaatagca
     1861 tccataacgt tcgatatctt gttgtcatta gaaagtgcct ttgcagactt tggatacttg
     1921 tccttgtctg taaaaagctt ttcaagcttt tcataagctt cgtcctcagt agcaccgtct
     1981 ttctcaaact tttcaataag cttttctata gtagtatcct tgctttttac ctcagcactg
     2041 acaacataaa gatttgatat catgttgttt gagtctgtaa gctcctcttt atctgcgttt
     2101 gaaacgtagg taagaggtcc gtaaataaca gggataagag caaacagaat gaccattgtg
     2161 atgaccattg gcaaacagct tgccataggg tttacgccag ccttggcgta aaggttcatc
     2221 tgctcttcct gcatcttctg ctgattttta gcgtatttct tctttatctt ttcaagctca
     2281 ggctgaagca tggtcattct tgccgtgctc ttctgctgct tgacgttaag cggaaatact
     2341 ataagtcttg taaggatagt gaaaagcagc agtgctattc cgtagttctt gcatacaaaa
     2401 tagcagccct tcattatcca gcccaaaggg gtcgcaataa tacccattta atatcctcca
     2461 gatcaattat ttgtcataat gataacctat atatctgatt aagctctcag gcttattttt
     2521 ttctgatttt aaaatactca gtggtaagct tgtcaggaac atagtcaacc cctcccagac
     2581 tccacgggtt gcacctaagc agtctccata cagtgagcag cgtgccttta aaaaagccgt
     2641 gttttctgta agcctccagc gcatatgccg aacagcttgg ataatacttg caccttggtc
     2701 cgaaaagagg acttataaat cgctgataaa accttatcag cagctgagcc agtttactca
     2761 ttttactttc tctttgccct gcctgacctg ttattctttg gaacaggctt attcatctct
     2821 tttatgagtc ttttttttat aaatctttca atatcctgag ttttcttttc ccctatatcg
     2881 tttcttccca caaaaactat atcatagccc agcggaaatt ccgcctcatt aagtctatag
     2941 gcggctctta ctattctctt aattctgttt cttgttaccg cgcagccctg ctttttgcct
     3001 gccgttatgc caagcctgtt gtatgggctg ccgttgggat aaaaatatgc tgctacaaaa
     3061 ctgtctgcac aaaatcttcc ctttttatag caccttaaaa atgccttgtt atcctttaaa
     3121 accgttgtga acagcatctt catttcccca ttgtgtactg tttttactct tttcgccttt
     3181 tcacggcaat tttgccaaaa aaagataaag acccaatacc atgcaaatca atgaatggta
     3241 tataggtcat ctgaggcagc gtccaacgac gcttaaatac aactgcctag ggacatcgat
     3301 tcaggcgtaa ctaaccgcct gaataatgcc tgtcagactt atcatcagaa actgcctgta
     3361 tctcttagta gctgagtcta gctctgccct ttgctcttct tcttgcgagt acctttctgc
     3421 cgttagcagt agacattctc ttcatgaagc cgtgaacttt ctttctctga agctttttag
     3481 gctgatatgt tcttttcatc gaattctcct ccttccgctt tcaacactcc gtcattttat
     3541 acaaaaaaac gtcctcggag atgtttaaac ttatagggtc acagcaaatt acaatatcgc
     3601 tatgaaaaaa gcccttgtaa tttaatatta tagctcattc acacaccatt tgtcaagcct
     3661 gttcataata tttttatatt ttttttgata ttatttttct ttgctttgag gggcttactg
     3721 tccgaaaaac ctgccgctta cgtcctcatt ttttatgagc atacaatata ttgtgtgcca
     3781 aatataaatt ttatctgtat taagtgtttt ttcttatctt tgaaaaacat
//
LOCUS       DAWEMR010000226         3748 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8508_length_3748_cov_7.627945, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000226 DAWEMR010000000
VERSION     DAWEMR010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8508_length_3748_cov_7.627945"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2168..2177
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2610..2642
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1111 a          736 c          939 g          919 t
ORIGIN      
        1 atagaatagg tatgtgtgca atatattgca cacaactgac agaattattg aaggagttaa
       61 tgatatgaca aaagcacaaa gatatttcat ggaaataaaa gataagagag tagcgttcat
      121 aggtacgggc gtaagtcacc ttgagcttat aaagcttttt ctctcaaagg gcataagatg
      181 cgttgtctgc gacaaaaaga cagaggacga atttgatgag cttatctatg aggagctttc
      241 cgcaaagggc tgtgagttct cattgggcga gcactatctt gatatcatct ttaattgcga
      301 tatagttttc agaacacctg gtatgtacta taatgatgaa actatcacaa aggcaagaaa
      361 agcaggcgtt gccattacat cagaaatgga aactttcttt gacctctgcc catgcaagat
      421 atacggtgta acaggttcag acggaaagac tactactact acccttatca gcgaaatgct
      481 caaagccgag ggcaagcgtg tccatatcgg tggaaatatc ggcaaggctc ttctgcctat
      541 agttgaaaca atgtcagaca gcgatgttgc agtggttgag ctttcaagct tccagcttat
      601 ctcaatgaga cagtcgccaa acgttgccgc aatcacaaac atcactccaa accacctgaa
      661 cgttcacgga acaatggagg agtatatcgg tgcaaaggcc aaccttatcg ctcatcagaa
      721 cggatactca agaactgttc tcaacgaaga taacgaggaa accataaagc ttgcagacct
      781 cgtaagagga aagcttgtca cattctctct aaagcaccct gttcagacag gcgcatatct
      841 taatgacgac ggtatgcttt gctacacgga aaagggcaga actacagaga tagtgcacaa
      901 ggacgatata aggatacctg gtatccacaa cgttgcgaac tatctcacag ctatcgccgc
      961 agtatggggc gaggtatcta tccagagcat agttcaggta gcaaagaact tcggcggcgt
     1021 tgagcataga attgaatttg taagagagat agacggtgtt aagtggtata acgattctat
     1081 cgcaacaagc cctacaagag ttatcgcagg acttaattca tttaatcaga agcttattgt
     1141 tatcgcaggt ggctatgaca agaagatacc ttttgaacct cttgcagaac ctgtgaacaa
     1201 gaacgtaaag atactcattc ttcttggtgc gacagcagac aagatagaaa aggctgttac
     1261 agaaagcccg ctctatcctg aaagcggtct taagatagta agagtaaaga cacttgaaga
     1321 ggcagtcctc acagcacaga aaatggctga aaagggcgat gttgtcacac tttcaccagc
     1381 ctgtgcaagc tttgacctct atccaaactt tgaggcaaga ggcagacatt tcaagcgact
     1441 tgtgaacgaa ctttaatttt ataaaaagca gggatatgta ttatggatac atttgagata
     1501 ctgtcacagc tttgctctgc ggcaggtgta tcgggagcgg aaggctcagc ggctgagact
     1561 gcggcaaagc tccttgaaga gtatggcgag gtcaccttgg ataacgctct tggcagtgtt
     1621 atctgcaagg ttggaagctg ggaggacggc aagcctgttg tgcttcttga cgcccatata
     1681 gacgagatag gcatgatagt cacatacatc acagaggacg gctttttgaa aatatcaggc
     1741 tgcggtggcc ttgatacaag gcttcttttg gctcagcagg tgacagttta cggcaaagaa
     1801 aagcttacgg gcatcgtgac ttcaactccg cctcaccttg aaaaagacag ctctgtcgca
     1861 cctgaccttg acagcgtttt catagatata ggtttcacga gccgtgaaaa tgcagcaaag
     1921 tatgtttccc ttggggacag agtgcttata gaaaacaaag ccgaaaggct cgcaggaagc
     1981 cgtgtgacat ctaaagcaat agacgatagg tcaggctgta cggctatcct aaagactttg
     2041 gagcttctca attgtcagca gacagcttat aatatcgcag tgtgcttttc aactcaggaa
     2101 gaagttggcg agcgtggtgc aaaaacagcc gcctttgata tcagtgcaga ctatgcgata
     2161 gtggttgnnn nnnnnnngtg gttgacgttt cctttgcaaa gacccactat gagagcgagg
     2221 aagagtgcgg cataatgggc aagggagcga tgataggcat tgctccgtcc ctttcacgag
     2281 agatgtcaga tggctttatt agccttgcag aagaaaaggg cataccatat cagcttgaag
     2341 tcatgagcgg aaaaacaggt acgaacgctg acgctatcgg tgtgagcgga agcggaacaa
     2401 aagccgttac catatccatt ccagaaaagc atatgcacac gcctgttgag atagtggata
     2461 taaatgatat tgataatacg gctctgctta ttgcagaata tctgaaaaga gtgtagatat
     2521 ttatgttgga taaactgaaa gacctctgcc tccttgacgg catttcaggc gacgagggag
     2581 cggtaagaga atatataata gataaaatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnggataacc ttggcaatgt catagctttc tgtaagggca aaaagacgcc taaaaacaag
     2701 ataatggtgt ctgctcatat ggacgaggtt ggcatgatag tcacctatgt gaattctgac
     2761 ggcacgctga aagtgtcatc agtcggcggt gttgacccaa gagttgtttt cggcagacgt
     2821 gtcaaaatcg gcaggaataa cgtccttggc gttgtggggg gaaaggctat ccataatctc
     2881 acagccgagg agagaaaaaa gtctgtgcct tttgataagc ttaccataga tataggtgtt
     2941 gacagccgtg aggaagcctt aaagcttgtg cgccttgggg acagtgtgag atttgtttct
     3001 gactttgtgg agttcggcga gggctttgtg aagtgcaagg ctatagacga cagagcaggc
     3061 tgtgcaataa tgctcagaat gatagaagag ggcatggagt atgacacata ttttaccttc
     3121 gtggtgcagg aagagatagg tctgcgtggc tcgacctgtg cggcgttcac tgttgcgcct
     3181 gattatgcag ttgtgcttga atccacaact gcggcggata ttccatcggc ttctggagag
     3241 aaaagggtat gtgagcttgg aaaaggtcct gttgtttcat atatggacag acacacaatg
     3301 tatgaccgtg agctttttga gcttgcattt tctgcggcag aagagaacgg cataccatgc
     3361 cagaccaaaa caatggtggc aggaggaaat gatgcaggtg cgatacacgt ttcccgtggc
     3421 ggcgtaaaga cagccgcagt gtcactccct tgcagatatc ttcattctcc aagctgcgtg
     3481 ataaataagg cggactttga gaacgcttat gttcttacaa aattgatctt ggataggact
     3541 tataacaaat gatctatgaa accaaagaga tcagctcaga actgctgagc ggtcttaaac
     3601 ctaacaatta tacaaggcgt ataaagtcgc actttgcggc atacggcaca gggtatgatt
     3661 ttctgcattt ctatgccgtt gaggaaagcg gagaaaagct tggtatcata tcagtattca
     3721 acgcctctat gatgatatcg actttcaa
//
LOCUS       DAWEMR010000227         3718 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8584_length_3718_cov_8.150696, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000227 DAWEMR010000000
VERSION     DAWEMR010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8584_length_3718_cov_8.150696"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1283 a          752 c          715 g          968 t
ORIGIN      
        1 gtctcaatta tgatatacgg aggaattaaa ggatggaaaa acaaatcgaa gataggatct
       61 ttaaaatagc tttcaagaaa ctaaaatcca gcgtttatta cgataaaacg cagcttattc
      121 tcagaaacga tatcgtcagg tatgagcaga attcaaaaga taaaattgac agcaagttag
      181 aatctttatg cacccaattc aatgatgaca gcaaattcga aaaactaaaa gaattgataa
      241 aacgctcaat aagtatatcg gcatttccca agaagttaat taaaagtcaa acacccggtg
      301 tgataatcaa caagcaaacc agccaaacca ctgtaaacca aaatcaatat tttattgaca
      361 tgtgcgttga aggacacatc cttggcgttg tctggctcat gaatattggg tctaagcttg
      421 acaagctcgt ttatgaaaag tcatacggaa acagaatcag gaaaaagctg ataaacgagt
      481 taagtgatga gccgactttt tcaccgtatc tgtttgaacc gtatttcatg caatacgaaa
      541 gctggcgaga cacagcgctt gacgaagcta aaaagtacct tcatcagaaa aatgacgtca
      601 tgatacttac aatggaccta aaaaggtatt tttattccgt agatgtaacc cctttcgaca
      661 aaatgcttga agacgcaggt atcgataaat cagataaagc aaaatgtgga cttgtcaagc
      721 tcaatgaact tatgtatgaa atcgtaaata catacgccaa acagtttgga gaagaatttg
      781 aaagaagaaa cattcttcct attggatttc ttccatcaaa tgtaatctca aactggtgtc
      841 tgcgtaatct tgacaaggca ataattgatg ggtggaatcc cgttttctac ggaagatacg
      901 ttgacgatat actcatcgtt gacaaaatag aaccaggcag caaaatattt aaccttattc
      961 gcagcgaacc taaaaaagtc aatgtggact tcattatgga ttattacact gaaaaatgct
     1021 gtaaatggta tgaccgaaac aaagacgcaa cgtcatgtaa agggcttttt ttcaagtgtg
     1081 atcaatcagg atatccctta tcgaatccag atgcaaatga tgtatattat agggtaaatt
     1141 ttaattttaa ccctacgatc ccggatacca aaagcaaatt gatcgtccag aatgaaaaga
     1201 tgaagctgtt ctacttcctt gagggtgaaa cagacgccct gatagattgc ttcaagaatc
     1261 agatttccaa aaataaaagt gaattcaggc atatgcccga ggatgacgca ttttaccaga
     1321 aagaccagta taacgagatc tactcgctgg tcattgataa cggcataaac aaattccgag
     1381 aagtcaagga ttgcactctt gataagttcg ggctgtccaa atttctcggg aaacatcttc
     1441 agattgccgg aatggtgtca ggtttcaatg agtcaaaatt tgaacatgat atcgaaaaga
     1501 tatttacacc gcatgtcact cttgaatact acattttctg ggaacggata attgaaacac
     1561 tggtgatcaa cgaaaattat gatgcacttt ataaattctt aagcagagtt gtttctgcaa
     1621 tatcagagat gagttgttct aatgtcgaaa atgacccaaa gaattatgac ccgaattggg
     1681 tggacagaac atcaaacata aaacgctcaa tgtataagta cttaatctcc gcattggcac
     1741 gctcatactc attgctatgg aaacaagacg ccttaaagtc agctataaaa attctggaaa
     1801 tcaacaccaa cgataataaa tcgatattag gaaattatct ggaaagaaca aatagcaaat
     1861 catcttctct ggagaatgtt ttttccaatc tctgcttgtg gcactgtaaa tcacgcatga
     1921 ttgacaagtc tgtacttcct gtccctatcg atttacttct gcctttttgt acctctagag
     1981 gaaataagct tggcattttt tcaataaaaa atggcatcaa tattactaat tttcaggacg
     2041 ccctggaaac tatgctttca ctcgaatcca aatcaaataa aaccgggaaa gataaaaagg
     2101 gtgctctgtg gccggatgac ggtaacaaaa caattaccaa gcattttaat tatatctact
     2161 atccatacat ggtaggaatg aacgaaatat caattgcagc ctgccttata ggtatttcaa
     2221 atgacgggat gcttgatctg ctgactgatg atcataccaa cgcctatgcc aatatcaact
     2281 ataacaatga caaaacatgt gattctaata tcatagaaat ctccaagcac acactcagcg
     2341 gtcaggagat caaaacaata tatgttggga caaacaaaaa gagcaaactt cgcatagctg
     2401 ttgcaaatgc gtgcctgttc aactttaaca taactgagct ttataatggt aaaccgcaga
     2461 ggagctactc aagatatcag gcactaagca aaatcgtcaa tcaggcaata aatgaaaaag
     2521 ccgatatgct ggttctgccc gaagcatatg taccgttcga gtggcttcca aggctttcta
     2581 aaacctgtga aagaaataat ctggcaatag tgtgcggcgt tgaataccta ataaatccaa
     2641 taaccgaaaa ggtttttaac ctcaccgctg taattcttcc gtttattgac aatgttttct
     2701 caggcaccaa tatcactttc catctgaaaa accactatgc tccagtggaa aaatccacaa
     2761 ctggacctag aggtctgcaa cttgtccagg gaaaacaata cgaattatac agatggaacg
     2821 gctgttattt tgccgtgtac tgctgttatg agctagcttc catacgtgat cgtgcgctat
     2881 tccaggcgta tgcggacgcc ataatagcaa tcgagtggaa caaagatgtg aattattaca
     2941 gcaacattct ggaatctcta agcagagatg ttcactgcta ttgtatacag gtaaattcag
     3001 ccgactacgg cgacagcagg atcacccagc catcacctac tgtttccaag gatcttgtcc
     3061 gaacaaaagg cggcaccaat gaaactatcc tagtaggagt tattgatgtt actgagctaa
     3121 ggcgattcca gatgcaggga caaacatttg tttcacccaa cagcaaattt aaacccactc
     3181 cacccaattt cgatatcgaa gtagtaaaac aaaaatacaa caacaaaccg cttcgatttg
     3241 acgtactcaa taacagtgat taacatacaa ctgataagta aagcatacac tggaggtgcc
     3301 gttaaataaa caacaccttc agtgcattat tctatgtgac tgaaaaagaa ctgtgcaaat
     3361 gcaaagccac tcaaacattt caatgctaaa aggcagcgaa acagggcaac gctggagagt
     3421 gacccgacgc ggctgcaaga tatacggtga atagcagcgg agtcaattcg ctgcttttca
     3481 tacttcaccg gtatcttcca gcggataaac tccgaggttc ggggcagagc cccgaattgc
     3541 tcctaggctg cgaatctgtc ctcaaagtaa atcatgaact gggcgtaagc cagcccccag
     3601 tcgcgaacag gcatagtcca cttcttcgaa atctcggaaa cgctcatata aacgaccttg
     3661 cggatagcgt catcggtcgg aaaaatcgac ttggatttag tgaattttct gaccattc
//
LOCUS       DAWEMR010000228         3684 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8679_length_3684_cov_18.070543, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000228 DAWEMR010000000
VERSION     DAWEMR010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8679_length_3684_cov_18.070543"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1129 a          737 c          876 g          942 t
ORIGIN      
        1 aggtctttca aaaggacttg gtttagagcc ttttatgtac tcggtttcag ataacaagct
       61 ttccacaaga tatgagaggc ttattacacc tgcaatgaag agcttttaca acgaacgcag
      121 agagaaatat tacaagtctt tcacgcaggt cagtgacctt tctcagtaca tcgacaatat
      181 gatatacttc acatttattg cagaaaaaga aaaacttgta ccgctgtatg acgctcttaa
      241 agaaaatgaa aagctgaaca tcacatacta ttatgatgtt tatgacccca agctttggta
      301 tcttgaagtg ttctcttcga tggcttctaa ggagcaaggc gtgaggtatc ttcgtgagaa
      361 ttacggcttt gactttgtca cggcttttgg cgacaacacc aacgatctgc ctatgttcaa
      421 ggcggctgat aaaagagtgg cagtaaaaaa cgcctgcaaa gaggtgcttg aaggctgcga
      481 ccaggtgaca ggcacaaatg aggagaacgg cgttgcagag tatcttctga aaacttttga
      541 aaggaattag ttatggcaag gatatatcca cttttttcat caagcaaggg caacagctct
      601 tttatcggca acagtgacgg cgggatactt gttgacgcag gagtatcctg caaacgtctt
      661 tgtgaggctt tgaaagctaa cgagatcgac cctgctgtga tacagggaat ttttattaca
      721 cacacacatt cagaccatgt ttcggggctt aaagttctca cgaagaaata tcacatccct
      781 gtatttgccc aaaagaccaa tcttgagata cttgtatctg acggcaaaat agacccagcc
      841 tgtgatgtca acgaggtgga cggcaatgaa gtctgcatag gagattatgc cgtaaaaggt
      901 tttgccacat ttcacgatac ccctgcaagc tgtggatatc agataaagac ccctgacggc
      961 aagcaggtat gcgtatgcac cgaccttggc actgttacaa atgaggtgaa tatgcacctg
     1021 agcggttctg accttgtttt gctggagtca aattatgatg agacaatgtt gaaaaatgga
     1081 ccttatccat acgagctgaa aaggcgcatc gcttcagacc acggccacct ttcaaactct
     1141 gcctgcggag aacagctaaa aaagcttatg acagcaggta catataaatt tattctaggg
     1201 catttaagtc aggaaaacaa cacgcctttg caggctgaaa actcggctgt gggagcgctt
     1261 ggggattacg agcgcaacag cgactatatt ttacatatag caaagcctga gggcagcgga
     1321 atggcggtgg cattttgata aggataaatc ttattacagt tggaaagctg aaagaaaaat
     1381 actggcgtga cgctgcggcg gaatactcaa agcggcttgg ggcgttctgc aagcttgaaa
     1441 tagttgaact gaacgagtcg aggttatctg acaacccctc cgaaaaggag atagcggcgg
     1501 cgcttgaaaa cgaggcgaaa gccatgaaaa gctacactga catcaagggt gcatacaata
     1561 ttgcaatgtg cattgagggc aagcagcttt caagtgaaaa gctgtccaaa aagctcagcg
     1621 aatgcggtct gaacggattc agcacagtga atttcataat aggctcttca ttcggcattg
     1681 cccctgagat aaaggcaaaa gctgacctgc gcttatcaat gtcggagatg acgttcccac
     1741 accagcttgc aaggataatg cttttggagc aggtttacag ggcgtttcag ataaatgcta
     1801 atactaggta tcataagtga atttcggata tgacaaaatc gccatttgtg cctgtcatgt
     1861 agtgatgaac atcaggatcg aacagcttac tttcggtctt gctggtcaaa aaatcattgg
     1921 cattgataat ttcaaggaca agcttaaaac ggctgataaa aatatcgttc agcttgtatg
     1981 cacagctttt gctgtccata gaatcatctg taattgaaat atgaggattt cttgacttag
     2041 tataacagta ttataagtca ttgcaatacc aacgtcaatg acttaaacta ataatataat
     2101 aattcagtat gtagcggagg tataacatga aaaaaatatc aatggtaata aacgcagacg
     2161 acaggctcgg acatatctct cccgagatat acggacattt ttctgagcat ctcggaagat
     2221 gtatctacaa cggtatatat gtgggtgaaa attcacctat tccgaataca gacggaataa
     2281 ggaatgatat catcgaggcg ttcagaaata taaaagctcc cgttttccgc tggcctggcg
     2341 gctgctttgc ggaagaatat cactggcagg acggtatcgg tgaaaaagcc ttgcgccgaa
     2401 agatcgtgaa cacgaactgg ggcggagtta cggaggataa ttctttcggt acacacgaat
     2461 ttatgcgctt ctgtgagctt gttggctgca agccttatat aaacgtcaat gttggcagcg
     2521 gcagtgttcg tgaaatgtcc gaatggatag aatatatgac ctcagatgcg gaatcgcctc
     2581 tcacagagca gagaaagaag aacggcagag ccgagccgtg gaaactcgaa tacctcggtg
     2641 tcggcaatga aaactggggc tgcggaggaa atatgcgtcc cgaatattac gccgacgttt
     2701 ataaaaggta tcagacattc tgccgcaatt attcgggaaa cagactttac agaatagcct
     2761 gcggtccaag ctctgccgac tataactgga cagaagtcat gatgaaaaat cttgacagca
     2821 ataatgttga tgctatcgac ctgcattatt atacgatgcc cgtatggccc gaaatggatt
     2881 cagccaccga ttttgacgat gagctttatt ataaaactat cgctgcagcg aacttttccg
     2941 atgagcttat cacaagacat tcggagataa tgaatcgcta tgatcccgaa aagaaaatag
     3001 gacttgtaat aggcgagtgg ggctgttggc ataaggtaga agagggtacg aacccgggat
     3061 ttttatatca gcagaataca atgcgtgatg ctatcgttgc tgcaatagag ctgaatgttt
     3121 ttaacagaca cagcgaccgt gttgtaatgg caaaccttgc gcaggcggtg aatgttctcc
     3181 aatcggtcat tcttacagag ggcgataaga tgataaaaac gcctacatat cacgttttcg
     3241 acctttttaa aacacaccaa aacaacgagg cagtttactg ctatacgcag aacgaaaata
     3301 tgtcggaagg caaaaatgct ccgatgatct cggtttccgc atcggtgaac gaaaagagta
     3361 tgaccgttac cgccgcaaac tgttcgctta cggaagaggc tgctgttgaa tgcagcattt
     3421 tcggatttaa agcatcatcg gtttcgtgca ggatacttac aaacgaggcg cacagctata
     3481 acgacttcga ctgtcccgac agggtgagca ttaccgagca tacggcagtc atcaaggata
     3541 atgttttaaa gctgaatatt ccgccttgtg ctgttgtgga atgtgttgtt gactaaatat
     3601 tggggcatat tcacagtgca tttcgtcttt ataataaaaa tataagcata agagctgcgt
     3661 ttcattaaag aagcgcagct cttt
//
LOCUS       DAWEMR010000229         3634 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8828_length_3634_cov_16.051132, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000229 DAWEMR010000000
VERSION     DAWEMR010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8828_length_3634_cov_16.051132"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          876 a          925 c          799 g         1034 t
ORIGIN      
        1 gagacttccc aactgcttga ttaagaagtc ttttagagca tacaacaccg gcagcgagct
       61 tatacgcctt gaattgtttg agtgcaaaag cacccaagcg ccgttgttcg gcatcgggtt
      121 ctgtttcaaa aagatcggca tagtctgcca ttacttcggg atcactatgc tctgtaccgt
      181 tcagccaaca ctctacaatt tcaaattcaa ggtctatcgc attcttgaac tcctcatcgt
      241 cctcacggca ttccgaagcg tttatcatat caataagcgt ttcaaaattc cattgttcag
      301 gatcatacat cgcaaagata agagcgatat atgccgtgta caggagcttt tcggctttta
      361 cccaaaagtc atcgccggac ggctgctgag aggaagaagt gttttttatc aagacctcga
      421 cgaatttcag aatgtctttt tcacggttct tttcgctgat gtacgcaaac gggttgtagt
      481 gcatcgactt tgcaaaatca atggtgttga acaccttgat cttatacggc tcataaacaa
      541 tctgtctctt gccgtccttg tccactgtat acagcacctc gccgtttttg ccgcgcgctg
      601 ggcggccccg tgcaagcatt tttccgcact cgaccaaaac cgttcccttc ggatcggtaa
      661 ctacatagga ggagtgcatt tgcatcaggt tcggcttcac gaaaaagcgc gttttacctg
      721 aacctgaacc gccgataacg aggatatttt tatttctcgc atacttcggt tcagagggct
      781 tgcccatagt cagagattcc gttttcgtga gaatgacgtt gttatcggga tttgagcagt
      841 ccatatacgg ctcaatatcc ttttcaccgc cccatacggc agagccgtac tcctctccct
      901 gacggaattt cttcttgttt ttcgacttga catagaggac taacctcatt acacccgcaa
      961 gaaccaagcc gaaaagaaga tcaaacgggt gtaaactggg aaagattcgt gcaaacgcca
     1021 caccgagatt gctcataaag ggcaggagct tttcctgaaa tccattgccc tcagcggttc
     1081 ggtacgcaaa gccgatcagg ttgcccacat aagagaaagc tacataggga acagccttga
     1141 tgatcagctt tttgagcttt ctggtgttaa gctgcaatac gcagtcttat cctttccgca
     1201 gttatcggca caaaagtctg ccgtaaaaat atcatcgccc cttgcccgtg gaacgggtgc
     1261 ggacttgaag tagtcataga tcaacgtctg ttctcctatg tgcgattctt cgcacggttt
     1321 ttctctctct gtttctgttt acgaggtctg tttgccacct tctgagcctg ctggtgcatc
     1381 tgctcacggg taaactcgcc gcgcttctgc gatttgccct gtcccttgcc gagatactcc
     1441 gaaaatgctc tcttgaaatt ctccgtttta gcagcggaga agaaaacatg ataggtaggc
     1501 ggctgggtgc ttttatctct tttcagagca aaatccacat cgtattttcg tgcggttttc
     1561 aggaaatcac cgatattccg gtctgaaacc tcaatgctgt cgagcttgga cggggacttc
     1621 gcctgcaact gcttgtaggt cattcgtccc tttttctccg ccttacccga cataaactcc
     1681 tgcaaggcag atttcagcac atcggcggtc agtttctccg ccttgatcga aatatctatg
     1741 gttttcttag caccgctttc gtagtcggac ggcattacgc atcaccgccc gtgatctcat
     1801 aaacggcata cgcctcggtc gaaagccagt catgaagggc tttgagtgca ttatctgctt
     1861 catcgggcgt gtcatagata cccataggaa tcttgttgcc agcggtatcc gtgccgatca
     1921 tttcaaaatc gggcttgatc gtgccgtttt cctcgtcaat ttcggcatca tcccaatttg
     1981 tttcaacgcc gatcttcacg atgttggcat agttcaggaa ctcgccaaac tgcgtaacga
     2041 tagtcttagc cattgtcctc accatccttt ccgtaaagct ctgagagctt gttgtgatac
     2101 tcctcctgcg tttcatcgag atcgcagaaa acgcagctca gaagattata gctgacctta
     2161 tcaccgagca tatccataac gatagcttca aattcgctat gaaaacggtt aaaaagtccg
     2221 tctatctcct gaaaagaagg attatcgggc atagttacac ctcctcattg gtctgctgcg
     2281 gcttcggatt gaaatatccg gtcttgagat atactaccct gtcagggcag taatagcgat
     2341 gctgtttgcc ctcaccgcag gggatcgcat aaccgaagtc aagaactccg gacagaatac
     2401 catttttcag cgtagtaata ccgataccgc ttgcccgtgc gatctccgac agaggaacat
     2461 ttcttcccgt aaacacaaga tcatgctcac tgattctctt tgcacccata ggaacacctc
     2521 ccttcgtaat cagacagccc gaccgtacat ctctgccaca gcattgtcag cccatcggct
     2581 ttcacagcct tcgttgaaat gatatgtcag gacttcgttg tagagggcgc tgatgaaata
     2641 tttgcggaaa ttcttgatat tatcgacctt tgccatattc acaaggacat tctcgaccat
     2701 gtaaatatta gccctaagca tcttggactt tatcacgctt ctggggaaat tcgtacctct
     2761 gagcgtggtg tacggcagag tagagcatat ttcgtcaacg ataaagccga cgatattatc
     2821 tgcttctgca tagccgtccc gaccgtcaac ggtcagccag tctccgagat agccgtagcc
     2881 gatgttctgt ttcacgactt ccgtataata ctcaacttct tctgcgttat atccctcgtt
     2941 cgacttttca acattttcac cgttgaaaac ttgtggaatt gccgcagaag gaatgataga
     3001 ggatttatca ctccgtaatt gatttttctg taatgtattg ttgttattat attgtccgtg
     3061 gttttcagac agcggagttt cagcagacgg aatgtccgga tacaggtttt ccaccctctg
     3121 tttttccccc tcagaagaaa taggcttttc agcccgaccg cctgcacgta tcgtgattgc
     3181 cttgcgtctg gtctcagctt caagctcgtc agcctgttct ttggtgacag gcacatcgaa
     3241 aaagctgtaa acgtactcaa agcagttgtg aacgctctca ttggagagaa gctgtgtgcg
     3301 tacaaggtaa cccatatcta tgagcttgtt tacagcgtta tgaaccgcag tcctgccctc
     3361 tttgcagatc gctttcagtc ccgacataga gaaatgccag tagtcaggca gagaacgcac
     3421 tttgagcagc aagccgagga gcttattggg aatcgtcttg ttccgcagga gagcagtgct
     3481 taccatagta aagttgctgt cctttttgac acgattcaga acggcgttat ccaccgtaaa
     3541 ggtttcaagc tcatagtcat actgagggat agaggtatcc ttcgctgaat actcatagaa
     3601 cttgtagacc gtctgtatcc tgccggtcgg gttc
//
LOCUS       DAWEMR010000230         3612 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8893_length_3612_cov_10.242339, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000230 DAWEMR010000000
VERSION     DAWEMR010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8893_length_3612_cov_10.242339"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1010 a          814 c          568 g         1220 t
ORIGIN      
        1 atcaagcgac gaataaaaat tattttttta tatttcacaa aaaccatcta cggtatatgt
       61 ttcttcgtca gatagtttta tcattgtttc ttctactttt gtcatatcat tgtctagttt
      121 taatataatc tcaaaacttg tatcaatatt aatcgacgtt ttattttcct ccaaaattaa
      181 gatctataat atttagatgc ccataattaa aagaaagagt cagccacttt ttatcaatta
      241 aatataaata tgagctaaag tgacttgaat cttcatgatt gtctgtcggt tcaccaaaca
      301 tttgatttat ttctgcttct gtactctcaa aggttattcc gcctattgaa atattatcaa
      361 tcgtatcctg ccttttaaaa gcactcgcag taaagcttat acaaataata tcattatcac
      421 tataatcatt atcgtctgct gcacaatgca ttgtgaccgt atccagcaat ttaccgtcat
      481 ataacaatcc tcagcttcta agttcggttt tctcctcata tatcatgctt tcatcccaaa
      541 aagaaaaacc ctcacccaaa tcatccagcg agcatggaaa tgaaaaagtt ttatcgaccc
      601 tgcgaaaaaa tctctgtaaa ttcaacaaca gtgataaaaa acgatattgt ggagcggctt
      661 gaaaaacagc cgctcatttt tttacagtat acaataaata ccgtaatttg tcaaggttct
      721 tcaacagtca gtctgctgga atggagcgta gcggaatgga agcagactga ctgtgccggc
      781 tcagcttagc ctgtcgagat acatgatctc cagctcgcca agaactatgc cccagtttct
      841 cagcggtata gtccattttt tcgctatttc atgagttgca agatacagaa ccttcaggag
      901 cgctgtatcg ctcggaaata cgcttctctg tttgttcaga cgacgatagc cgctgttaag
      961 gctttcgatc gcattggtgg tgtaaatgac ctttctcaca tcagcggaaa acttgaagat
     1021 cggtgagatc acatcccagt ttttgtacca gctcttcatg gcgtttggat aatctttctc
     1081 ccacttctcc gtcacacgct caagccgttc aagtgcagcg tcctcagacg gcgcctggta
     1141 aattgttttc agatcattag caaattcttt cttgcttttt tcgccaacgt atttcagcat
     1201 atttcgcacc tgatgaacga tacaacgctg taattctgtc tgcggaaatg ccgctgatac
     1261 ggcgctcctt tcatacccga aagaccatcg gcacatatca cgaggacgtc ctttacacca
     1321 cgatttttca gctcattcag gactccaagc caatattttg cactttcgtt ttctccgata
     1381 tgtatggata aaacttcctt gtgacctgtc aaacttactg caaggattac atatgctgca
     1441 agttttttat ctgtccgttt tctcgtacag aaaaatgtac agcatcaata aatacaatga
     1501 gataaacctc gcccagagga cgtttctgcc agtcctctat ctgcggaaga aggcggtctg
     1561 tgatatcgga tatcatactc ctcgatcagc tctgcgatga tattcttctt cccctcgctc
     1621 attggttctc tttttcttct tgccataaaa aatcagcctc ctgtgttatt tatattttac
     1681 catagttgac tgcgttttta cagacttttt ttcggtgggt ctcagctctt ttattataca
     1741 ctactttgga gatttacaca acatatggga gaaaacctat cttttctttg aatatagctt
     1801 ttaagatatt tcttagttag tcatattttg ttcttctctg atgccaattc ctgactttct
     1861 cttttgtatc atttttctga gctttctatc agtttgtatt tttaaagaac actgcttctg
     1921 cgaataatta tcctcaatac agcgacttaa attcacaaac aatcccataa cagtatttct
     1981 aagctgcata ttttcaaaat catcaatatt gttaagcata ttctgaacgt actcattacc
     2041 atcatgtgcg gactttgtga gccactccac agccttttcc ttatcctttt caagttcatc
     2101 tgctccgaaa agataaagcc tgccgagctg atagcttgac cacatatttt tatcagacga
     2161 ccgcttaaaa tagtccaccg ccctttgaat atcatacttt tcttcctgca aatacaactt
     2221 cccaaaagca tactgtgtga attcgttatc ctctgcaagc agcaaatatt tctctgctct
     2281 ttctaaatcc tgcgtgacaa tttcaccttt caaataaaac tgacccaact gaaaacaagc
     2341 aaaactgtca cccttatctg cacattctgt caacataaat agtccttttt caatatcttg
     2401 tgaaaaatat tcacccgaaa tatattccaa tgcaaaaaaa cgctttgcat tggaattttt
     2461 aagtttcatt gctttttcaa tacactccaa acccttattt aaatcagcct caatgtattt
     2521 tccttgaatt aatgttttgc catattcata cagaccattc ttgttattca tctcagcgga
     2581 acgtttgaag taatcaatgg cttttgaaat atcagcttcc gttccaagtc cgtttttgta
     2641 cataactcca attttataaa ataggttatc gtccgcctga tctttgcttt caagttcaag
     2701 gaaaccagat aatgcgacct tgtaatatct ttgtgcggtt tcttcacttt gacttacata
     2761 ttcgccttta tcgtacattt gagcaacagc gtatggggca taaggctgtc cctgttcaga
     2821 tgatttgcga taccacaaaa acgcctgcga caagtctttt tccacaccgt tgccgtagta
     2881 atataaattg gcgagactgt attgtgcgaa tttgttgccc tcctgagctg acttcaaaaa
     2941 ccattcaaat gccttttcat aatcttgttc cgttcccaga ccatagcaat gcatttttcc
     3001 gattctatac caaacgtaag aacgcatatc cacgggtttc atgacctgcc ctttgtattt
     3061 tggttcatat ggaaacataa aatctgagtc cggctcgatc tccataaaac cctgaaatgc
     3121 ttcttcgtag aatgaaaaag actttttttc atctttttcg ccgagtttat cagtagaata
     3181 gagtttgccc agatcttgaa tagcaagaac attacctgcc cctgattcgt taagcaaaag
     3241 ctgctccgct ttttgaaaat cttcaagctt tgatcgcttg ttataaatca gcttgcaggc
     3301 ttctttgtag gaactactcc acttgaggta atatttgctt ttcggagcgt catcaacatc
     3361 gactgttacc gcattttcac cactccaaat gaaatcttcg gctgtcagat catcgttatt
     3421 tgaaaatgtc actttatcat tttcggattg ctcagattca tcgaactgtg gagaaatatc
     3481 aacataaaag tcatcattcg caaattgttc tgttggttcg ggttcttcaa tttctacatc
     3541 aatgacagga taattcatgt caagcacagt tctgattatc atattccgca caggctgaaa
     3601 aactttgtta tc
//
LOCUS       DAWEMR010000231         3588 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_8954_length_3588_cov_12.897254, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000231 DAWEMR010000000
VERSION     DAWEMR010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8954_length_3588_cov_12.897254"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          837 a          962 c          794 g          995 t
ORIGIN      
        1 cgcaggcggc agggcagagc aaagtccaaa gcctgccctt gaaatggcac gtcagctaat
       61 aagcgagaat cctcacggag tgaatcttat atgcggttcg cttttcctat gtgcccaggt
      121 gcttaatgaa ataaaagatg attaacgtac atcagtaggg gcgacctcag gtcgcccgat
      181 ttttttgcct ttattcccta atagccgctt gaaccgcttt cttccaacca cctatcagat
      241 cactgcgttt accctcactc atagacggaa taaactctct aaacttatcc gaaactccca
      301 caagctcttc catactgccc caaagcccac agccaagccc tgcaagatat gccgcacctg
      361 ttgcggtgga ttccacgtta taaggtctta tgaccgaatg tcccaatata tcagcctgaa
      421 attccattag aaaatcgttg ctcgccgcac cgccgtcagc ctttatgctt ccgatagccc
      481 cagcgtcctc ctccatcgcc ctgagaacgt cataggtctg atagcacata gcttccaatg
      541 ttgccctgac tatgtggcat ctgtttgttc ctcttgtaag ccctgttatc atgcccctgg
      601 cttcgggctt ccagtatggt gcgccaagtc ccacgaatgc aggcacaaag tatacaccgc
      661 ccgtgtcctc aacgcttctt gcaagctcct cggtttctgc cgctgtgcgt attatgcaaa
      721 gctcgtcacg aagccatttt accgccgcac ccgatacgaa taccgacccc tccagagcat
      781 aattcacctt tcctcctatg ccccaggcaa tagtcgtgag caatccatga cggctttcca
      841 caggcttttc acccgtgttc ataagcagaa aaccgcccgt gccataggtg tttttcacat
      901 ctccctcacg gaaacagcat tgcccaaaaa gtgccgcctg ctgatctcct gcacagcctg
      961 cgatctttat cctgcttccg aaaatgttct cctcagtttc gcccaagtca cccgaacatt
     1021 ctttgacctc agggagcata cagctgggta tgcccaaaag ccccagtatc tccctgtccc
     1081 attcaagggt gtgaatgttg aaaagcatag tcctggaagc attggagtag tccgtaacgt
     1141 gtacccttcc ccctgtcaga ttccatatga gccagcaatc cacagtgccg aaaagtatct
     1201 cccccttttc cgcacgctcc ctcacaccct caacattttc aagtatccac tttatctttg
     1261 tggcagagaa ataagggtcg ataagaagtc ccgtcttgtc cttgaaaaac ttctgtaagc
     1321 cctgactttc cattcgtgcg cagtcctcag aagtccttct gcattgccaa acgatagcgt
     1381 tataaacagg cttgccggtg ttcttgtccc aaagtatcgt cgtttcacgc tggttggcga
     1441 tgccgatagc cgccacgtcc tccgctgtta tcccggcctt tgcaatgacc tcacgagcag
     1501 aactgagctg actttccagt atgtccatag ggtcatgctc cacccagccg ttgccgggga
     1561 atatctgcgg atattctttc tgtacgctcg caaccttttc aacctttgcg ttaaaaagca
     1621 ccgctctgga ggaagtcgtt ccctgatcta aagagagtat gtattctttc ataaaatttc
     1681 tccttattgc aaaaaagggg cggaattccc accccttata aatctgttat ctaaatttct
     1741 aacactgttt attttcctac aaaatttcgc atcacattat aatgtagggg acggcgccct
     1801 cgacgtcccg cttcctcgcc cgattaattg ccgaataaac ttgtgttact cgcaaaatgc
     1861 tgacgttttc ataaaccatg taggggagcg gctcaatttc tgctttgcag aaatgcaacg
     1921 tcccgcttgc ccgacgtttt gtttacttat tttttttcgt tacaccacgt tgcggatatt
     1981 ctgcaacact ttatatcgtc atcaaataag ccccgtgtaa agcagtataa gataaatgat
     2041 caacaaaagc gacagaacgc caagaagctt ccaaataccc tttatggcgg acttttcctg
     2101 ctttttagct ttaacaaatt cctctatagc gtccggagtg tattctttgt gcatctggct
     2161 tcctactgtt ggattgagca tatcattgat gttgatgttg cggtattttt cctcacgaag
     2221 cttcttagga tcggtctcct tggtgctgat gttatcatat gaagaattga gcaggctctt
     2281 gtccattttc ttcatgttga tagcgtccat agaaagctct gcttcctcag gtctgccaag
     2341 gctgtcacca ttgtcgtaaa tatgctttgt gtcaacgctc ttcataagct gattgtttga
     2401 gcggtttgcc ctcagcatct tgataagagt gtcagtgtag aggttttcct cttcctcagc
     2461 ctcgtcagta aggattatgt ccgaggtgtc gtttatcgct tcgtcaactg tatcaataag
     2521 ggaagtaagt cccttattcg ccttcttgta tggcttttta gccgcaggct tcggagcttc
     2581 cgccgcaggc tgtggagcag gctggctgta cacaggtgct gaaactgtct gctgtgcagg
     2641 ctgtgctgtc tgaacaggcg ttggagcagg agcaggctgt gaaactggcg gaacaggagc
     2701 agaaacaggc tggcttgcag gtgcagagcc tgaaatgccc tcgtgtcccg gaataaagat
     2761 ctttctcttt cttataggtg cgtttatctc gccgggaata ccgctctgag ccatcggctt
     2821 tgtgttgaat acaggagcag actggatgtc ctctgcaggc atcggtgcag gagcaggctg
     2881 tggctctggt ataggctctg gctctggtgc aggctgtact gccgcaggct caggctgaac
     2941 gggagctgga ggcggaacta ccttaagctc aggattttcc tcaggctcgt caggtatagg
     3001 ctcgctgtca gggccgtcct cgccaacgat agcgtcagca gaaatggagc ttgcagccgc
     3061 agagaaatcg ttgctctcga tagcagagtc ctcgtcgccc tcaaagctta tcattttgcc
     3121 tgttgtaaca tcatagtatt cctcgccgtc cttgacagcg atcttgagct tcggcgcagg
     3181 ctcgcccctc ttaggcaggt tagcctgtgg aatgtcaatg tcagggaata cctcgtcaac
     3241 gtccaccttt gcgatcttaa gtatctccat tgcggatctg cacttcatag agcctcttac
     3301 catttccttg ataggaagca gaattatatc gcagaatgtg gcacagaaag acctcagcgc
     3361 agacattgag ccgtcaagca tatcctgcgg aaaacgatgg tcaaaatact tatcccattg
     3421 ctcagggctt gacatgatca tcttgtctat gtactgacaa agcggtatcc aaagctcagg
     3481 ctcttcaaaa tctctgtcat tggtgatgag ccacatataa catctgagga aactgtcata
     3541 gcatgaaata tctctgaggt tgagcataac gtcatcaatg ttcaggga
//
LOCUS       DAWEMR010000232         3564 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_9011_length_3564_cov_8.461955, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000232 DAWEMR010000000
VERSION     DAWEMR010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9011_length_3564_cov_8.461955"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2340..2349
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2774..2783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1124 a          648 c          754 g         1018 t
ORIGIN      
        1 tgaccgcatt ttttgtcgca gggacatatc tccttgctga aaggcaggtg ggataatgaa
       61 aagtatgtat agttatcaga cttcacggct cataaagaaa ttctggctgc tttttgtcat
      121 agtgataatt ttttcagctt ccgcaaaatt cacagtgtat cggagctttg attcttcaaa
      181 taatcttacc tatatgaaca aagaaacggc ggagatatac aatgaactta tagaaaattc
      241 agcagacaag acacttgatc aatgtgatga atacataacg gactatgagc tgaggagtta
      301 cgcaggcggt tataacggta acatcacagc cgccgtgaac aagtataaga ccgagcttcg
      361 cagttgtttc atggtcgaaa gcatcagaag ctatgcacgc acgggaagag gtctaacaga
      421 cgctaatatt ccctctgacc ttgtgaaaaa cagccgactt tacgccgcct ttccaacacc
      481 gcaggtgata aatgacgagc cgtttttgaa gtttcttgaa ttgcaggcgg tagatatcat
      541 gcctatattt gtgcttctgc ttgtgggcgt ttttgttgca gatagttatg aaaagagaat
      601 ggatcagcag ataaagatat ctaaaaattc gtcagcgttt tttaagtcac gggagataat
      661 tttatcagtg gcgatattta taatgttcac actgagcttt tttgcagacc ttattataag
      721 cggaacatta catcagagca agtacagcac ctcatcttta caaagcgtgt ggcaatttga
      781 ccttgcgcct ttaatgctaa ctgttagagg cacaataata tggctatatg tcacaaggct
      841 tatgtcgctt tttataacct acggcatttt cgtgcttgtg gcaaagcaag tgcgctctgt
      901 gaaaaaatat gttatgctga cctctgggct gatagcggtc atgtccgccg ccgcacatta
      961 tgtgggagca ctttcgcctt atatgtttgc ggctgtcaca agcgtaaatg ggcagcttaa
     1021 caaccttgac tatatgaggg cttttgattg ttcggaagcg gtagctgtat tcatatatct
     1081 tgcagcgatt ttgagcggac tgactgcatg gagatacagc ggatattatg aaaaaagctt
     1141 tgaccgataa ggagtggtga tgatatgagc gttttaagag tagaaaactt atccaaaaga
     1201 tacggcaaaa agcaggtgct caagggcata acctgcgaat ttcatacggg cataaacgct
     1261 ttgcttggac caaacggcgc aggcaagacc accataatga actgcatatc gggcgtgata
     1321 aaaaagtcgg gtggcagcgt gaaccttgac gggcaggacg tttttgaaat gggcgagaaa
     1381 tatcgtgaaa agctgagctt tcagtttcag tatcagccat tttatccaag ctacaccgcc
     1441 catgaatttc tcagattttc ggctctgtta aaaggaatgc ctgagccgct tattgaggac
     1501 gagataagac gtgtgctggg gcttgtgaat ctgtctgaaa atcttgatga caagataaag
     1561 tcattttctg gcggaatgaa acagcgcctt gccattgcac agactatcct taactccccc
     1621 gagatactga tttttgacga gccttctgct ggtcttgacc cattcgaacg tgagcaattc
     1681 aagaggataa tggtgcgact taaaaaggac agcattataa taatttccac ccatatcgtt
     1741 tctgatattg acaccataac ggacgacctg atcatactta acaaaggctc tgttatcgct
     1801 aatgacagcc cagaggcgct ttttgagcat ataaggggta tggtttggga tattccgaaa
     1861 gcggacattg gtctgctgcc tatcgagaac atttattatg aggatacaaa gtcgaagact
     1921 atctcaaaga caaagcccac ccctaacgct gttatgagcg agccgactat gaatgatctt
     1981 tatttctgcg gacagcttga cgggggcgtg tttgagtgag gagataattc agataatgat
     2041 gtcaactctg aacgatggtt gcctcttatc ttcagtaatt aggctttgtc ctctacgaaa
     2101 aaattgtaat gaaaggatag aatgtgctta taaaccacaa ccaaaatatg ctttgtcttg
     2161 atgaatttga tatacttgaa ctgcgtgaaa atgcagatga gtgtgattat ctttttgttg
     2221 tttcgcctaa aattgaaaca aagatatgca caaaatgcgg gagctgcaat gttgtcaatt
     2281 acggtcgata tactaaactt gtcagggata tcaacataaa tgaacattat acagctcttn
     2341 nnnnnnnnng tcacaaacat ttgtgtaaag actgtggaag tatttttgtt gacagattca
     2401 atacacttga tctttgctca aaaatgacta acagactaag gtgcaagatc catcaattat
     2461 cattaacaaa actatttaag catattgcaa gagaatataa gttatctgtt actacgatag
     2521 agaaaatagc tttggcatat attcgagaga aagattctca gcatattatg tattcacctg
     2581 aagtattagg tatagatgaa gcacatgtga atggctcgat taggtgtgtt ttgacggaca
     2641 taaacgagca cttgcttttt gaaatgcttc caaagagaga caaacaaact ttaataaatt
     2701 atctcgactc attgcctgac agtaacaata ttaaagtagt tacaatggat atgtatagac
     2761 cctatcgtga ggcnnnnnnn nnngcttacc ggatgcggca atagttatcg acagatttca
     2821 tgtagtcaag ttaattaatg acgtactaag cgactataga aaaacacttt gcaaacagct
     2881 atctgctgct gaaaagaaag agataaattc agtacagaaa gtcttaaatg ccaacagaaa
     2941 agacttaact gcaaaccaaa acaaaaaact tggatacatc tattcaaagt atccggaact
     3001 aaaaatggca tataatctta aagaagaatt cagagagatc tacgaatgta ctaacaggag
     3061 agcggctgtt ctaaagtata caaaatggtc cagaaaggtc aaacagaact ttagtcctta
     3121 catttcaata actgaaacgg taaatcgatg gagcctggaa atatttaatt attttgatca
     3181 cccgtacaca aatggcgtta ctgaagctat aaatggatgt ataaaagcaa tcgcacgaga
     3241 tgggaaaggt tattcgtttg aaattctaag aggaaaggtt ctgtatggtc aagcctataa
     3301 aaccaatgcc attttttctt ataataaatt ttctccgaca gtacactatg caactggtcc
     3361 ttcgaaccgc tcgtcattaa atatgattca aaatcttatg gattcgtaca aacagatatc
     3421 aggagccgat attgataggt taaaattagg actattctaa ataggataag ctgccacaga
     3481 ttttttctgt agcagcttat ttttgtttgt gctattcctt cttgcgaaga tatccgtcat
     3541 catcatattt cttagctata tgct
//
LOCUS       DAWEMR010000233         3523 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_9145_length_3523_cov_11.175029, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000233 DAWEMR010000000
VERSION     DAWEMR010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9145_length_3523_cov_11.175029"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1072 a          744 c          799 g          908 t
ORIGIN      
        1 gtctacagtt atcggctttg ttctgggtct ggcgaccatg cttcttgtag caaagatagt
       61 tcaactttct gtaagacttg ctgaaaacgg tacggctgat tataaaaatg ctataggaga
      121 aactgctact gtttatatcc cctgcccacc taaagaccat gggcttggta aagtgaatgt
      181 tatgataaac ggacagctaa gagaagtcac tgcaataaac aatggtgatg agcttataaa
      241 gacaggaact caggttcgta ttatcgattt gcaaggagat actgtcgtaa tagaaaaata
      301 aaagaattga tagaacaaag gggtgctaaa cataaagatt ctgtgaaatg acccgtcttg
      361 aggttattat aatattattt gtgtgggagg aaaaattatg gaaccagtaa tcatcatctg
      421 cgtgatagtc gttgtgcttt ttgcggcaat tatggcgata ctgtcacgct acagaaaatg
      481 tccgtctgac aaggtgcttg ttatctatgg taaagtagga acagacaaga acggtcaagc
      541 aagaagcgca agatgtatcc acggcggtgc ggcatttatc gtgccaataa tccaatctta
      601 tgagtatatg gatcttactc ctatatccat aaatgtggat ctaaaaaacg ctctttcaaa
      661 gcagaacatc agaattgatg ttccgtcaag atttactgtt ggtatctcga cagaaccagg
      721 cgttatgcag aacgctgctg agcgacttct cggtcttaaa ctcattgaga tacaggagct
      781 tgctaaggat attattttcg gtcagttgag acttattatc gcaacaatgg atatcgagga
      841 aataaactca gacagagata aatttttgct cgcggtatca aataatgttg agatagagct
      901 taaaaagata ggtcttaagc ttatcaacgt aaacgtaacg gatattacag atgagtctgg
      961 atatctggaa gctctcggta aagaagctgc tgcaaaggct atcaatgacg ctaagaagtc
     1021 agttgccgaa aaacacagag acggtgagat cggtcaggca aacgctcaga aagaccagcg
     1081 tatccaagtt gctgctgcaa acgcttctgc tatcgacggt gagaaccagg ccaagataga
     1141 ggttgcacag tcagaagccc tcagacgtga gcgtgaagcg gaagctctca gacaggctac
     1201 agccgctgaa gctgttcagg cagcaaaggc aaagcaggaa gcttacatag ctcagcaatc
     1261 cgccgagcag acgagagccg aacttgaaaa ggctacacag accgccgatg ttatcgtaaa
     1321 ggctcagatc tcaaagcagc aggccgagat cgaagctgaa gcacaggctg aggtagtaag
     1381 acgtaaagca aagggtgagg ccgatgctat ctttgcaaaa atggaagctg aagcaaaggg
     1441 taatcaggaa atactcacaa agcaggcaga gggtatgaga aagcttgttg aagcagcagg
     1501 cggagatgct aatgcagcag tcaaactcat agttgctgac aagcttgaag aacttatctc
     1561 tattcaggta gaagctatca agaacattaa gatagacaag ataaccgtgt gggatacagg
     1621 tgcaggtgca gacggaaaag gctcgacagc aaactttcta agcggactta tgcagtcagt
     1681 tcctccgctg aacgatctgt tcaagcaagc aggaatgtca ctccctgatt ttcttggcga
     1741 ggacatacag gaagctttga aaaagtcaca ggaagaagca gtaaagagag cagccgaggc
     1801 tgataatgca gaaactctcg acccaaaaga tgttgaggta atagagcctg agaaataatt
     1861 aatttgacct ccgtagttta acgattgcgg aggtcttttg gtataaataa aagcgagttt
     1921 caacatcatg aaactcgctt tttatgttca atatggttca acgactactg ctctgccctc
     1981 tttcaaggac ctttgtacat caataaaacg ctgatttttg ctgcccatga atttaagctc
     2041 aagacttcgt tgtgacagta taaaagggcc gtccacaaga taatcaagct gttgcagcag
     2101 ctcaagatag ccgttattat catcagcatt ggctataaga tattcataag tatatccagt
     2161 gaaagatatc acattcagtg gaaagccatg gaacgctttt atccttcctg caagttctgc
     2221 taacggtttg cactgacaga aaggctcacc gccactgaac gtcacgccgt caagaagcgg
     2281 gtcttttatt atctcattgt aaagctggtc tgtgtcaacg acaatgccac cattaaaatc
     2341 atgtgtctgc ggattgtgac aaccctcaca gccgtgtgga cagccctgcg taaaaatagt
     2401 gaacctaaaa cccggtccgt ctacgataga ttcaccgaca gtgcctgcta tcctaagctc
     2461 catggctaat tgtcaccctc acagcaggct tttgcacgag gtatcctctc atgtatcacc
     2521 ttgctgtgtt cagcctcaga acgtccacac ttcgggcatt tgtcacctat tattccagta
     2581 tatccgcaaa caggatcacg gtcaacaggg tggtttattg agccgtagcc tatgccaact
     2641 tctttcatat atcttacgac tttctcgaat gcagaaagat tgttcagcgg atcgccgtca
     2701 agctcgatat agcttatatg gcctgcattt gtaagagcat ggtaaggagc ttctatttta
     2761 agtttctcaa acgctgttat cggatagtaa acaggtacat ggaaagaatt agtataatat
     2821 tctctgtcag taactcctgc tatctcgcca taacgttctc tatccatttt aacaaatctg
     2881 ccggaaagtc cctcagcagg cgttgcaagc aacgagaagt taagaccagt acgctttgtt
     2941 tcctcgtcca ttcttgctct catacgtgag attatctcca agccaagctc tcttgcttct
     3001 tcactttcac catggtgctt gccggtaagc actttaagac actcagcaag accgataaag
     3061 cccactgaaa gagtgccatg ctttagcact tcttcaagtg tatcgtcagc cgtaagcttc
     3121 tctgaatcga gccatatgcc ctgacccatg aggaacggat aatttctcac acgctttgaa
     3181 cactgtatct taaatctgtg cataagctgg tcgatacaaa gatccataag ctcatcaagg
     3241 ctgtcaaaga aagcaccgat attacgctgt gcttttatgc caagtctagg gagattaatg
     3301 gtagtaaaag agagatttcc tcttcctgtc actatctcac gagtagggtc atatgcgtta
     3361 ccgataactc ttgtgcgaca acccatgtaa gctatctcag tgtcaggatt gccctcttta
     3421 taatactgta gattgaaagg agcgtcgata aatgaaaaat tcgggaaaag acgcttcgct
     3481 gatacacggc aggcgagctt gaaaaggtca tagttcggat cat
//
LOCUS       DAWEMR010000234         3504 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_9195_length_3504_cov_6.664830, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000234 DAWEMR010000000
VERSION     DAWEMR010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9195_length_3504_cov_6.664830"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2304..2313
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1086 a          646 c          751 g         1011 t
ORIGIN      
        1 cacaggcagc taacgatgac ggacatgaga cttggcggac tcgggaagaa aacttactga
       61 gcctatggga agaagtaagg aacaatgatg ttaggatcac cgaaaaactc gtcatagacg
      121 ggcccttttc aaaggaaatc ggatcagata tttttacttc tgacgttaaa gatgaagttg
      181 ttttatgctt gaactatgat gggaaattcg ggttaaataa tataaacagt tatttccaaa
      241 atgcaaatcc aaatggagaa gctataatat ggcaaagctg gaggtttaaa aagggagaca
      301 aaattctctt taatgataac tctcgtttca catatcttta taataatctt aaagggatca
      361 tcgttgaaat tgaaaaggct gatgatcaaa tagcgtttac tattgatgtg gaaacaataa
      421 ttactgaaca acaatgtaaa tcagatcaaa tagagtacat tgacaccata gacgaaaaaa
      481 ctcggattaa actcattgta tatgctttcg atgaagatga agttgatgac gaggaaaacg
      541 ctaagaagac tattatacct ttccagttag catatgccgt ttcgattcat aaagcacaag
      601 gcttagaata tgattctgta aaagttgtta ttccgagcgt taataccgaa aaaataacaa
      661 aaggtgtatt ttacacagcg ataactcgca caaagaaaaa actaaccatt tactggagtt
      721 ccgaaacgat ggaagcaatt atttctggtt tttcatctga acataatggt tttaaaagtc
      781 tggaaatcat aaagaataaa ttagttcaga ccactagttc cagttaaatc taataacgga
      841 agtcgtttct tctatgtaaa aagacagata cctctcttgt ggaagtatct gtctttcatt
      901 ttcatagctc gctgttttgg tcttaatata gtagttggga ttatactgct atatcagcgc
      961 cgcactttcg gtcggggctt tttttcacat ggttttcact ttgcattaag gtctgtttaa
     1021 gtaaggaacg ctataatata gtcataacaa aaccgagacg ggaggcgttc ttgtgaacgt
     1081 aagtaatatg gctattctta ttattgcggc tgtgctgctt tctttgccgc ttgttttatc
     1141 tgctgcattt tgtgttgaag aacgctgggg ctttctttca cgaaaacatg ataagtggat
     1201 agacgaacat tacagataat aggcagacag ataagcatga gttgtccgtc atacaaatac
     1261 aagcgtattt cacaaacata caccaaaaaa actataaaaa ttagtccatt tatacattga
     1321 cttaatcacg caaattcggt atactattta ttgtaacgtc agcgtgggaa aagtacgcag
     1381 acaggattaa ttctatagtt tataaggggt ttgtgaaatg acaaaggttg taaaattcgg
     1441 tggaagctct cttgcaagtg ctgagcagtt caaaaaggtt aaggagataa tcacagcgga
     1501 aagctcaaga agatttgttg tgccgtctgc tcctggtaaa agattttcaa acgatacaaa
     1561 ggtaactgat atgctttacg gctgctatga tcttgctgca aagggcaagg atttcagcag
     1621 cgagttcgac gctatcaagg aaagatatta cggcataatc aatgatttgg gtcttgacct
     1681 ttctcttgac aaggagtttg aaactatcaa ggcttgcttt atcggcaagg cgggacgtga
     1741 ctatgcggct tcccgtggcg agtatcttaa cggtatcgtt cttgctaact atcttggata
     1801 taactttgtt gacgctgccg atgttatcta ttttaacgat cagggtcagt ttgattctgc
     1861 aagaacaaac gaggtgcttc ctcagagatt agctacactt gacaatgcgg taatacctgg
     1921 tttctacggc tcaatgccta acgatactat caagacattt tcaagaggcg gttctgacgt
     1981 aacaggttct atcgttgccg cagcagtaaa cgctgatctt tatgagaact ggacagacgt
     2041 atcaggattc ctggtatgcg accctagaat tatcgacaac ccacagccta tcactaccat
     2101 aacatataag gaactgagag agctttctta catgggtgca ggtgtacttc atgaggacgc
     2161 tattttccct gtaagaaaag caggtatccc aataaacatc aagaacacaa acaagcctga
     2221 ggatatgggt acaatgatcg ttgagtccac atctcagaag cctgcattca cgatcacagg
     2281 tatagcagga aagaagggct ttannnnnnn nnngaaaagg atatgatgaa cgcagagctt
     2341 ggtttcggca gaagagtgct tgaagtattc gagaagaatg gtatctgctt tgagcatatg
     2401 ccttcaggta tcgacacaat gtctgtcgtt gttcatcagg cagagtttgc tccaaaggag
     2461 caggagataa tgtcaggtct gcacagaaac tgtcaccctg atacaattga tattgaaaca
     2521 gaccttgcgc ttattgcagt tgtgggacgt gcaatgaagt caaacagagg tacagcaggc
     2581 agaatatttt cagcccttgc acacgctcat gtaaacgtaa agatgataga tcagggctct
     2641 agcgagctta acatcatcat cggtgtaaac gaggctgatt ttgaatctgc aatgaaggct
     2701 atctatacaa tttttgttga tacacagctt taatacaata tgaaaaatat ggcggacggc
     2761 tttggctgtc cgcttttttt gtgcaaaaaa gcgtcccgaa aattatcgga acgctcttat
     2821 ttatttcttc ttgtcagaaa gctcagtgat tatctgaatg aacgaatcaa cgtcagagaa
     2881 gtccttgtaa acggaagcaa aacgcacata tgcaacgtgg tcaagctttt tcagacccat
     2941 aagcaccata tcgcctattt cgctggtggt ggtctcagtc tgcattttgt ttgcaaaggt
     3001 attctctata tcgtcaacaa ggtttttcat atcatcaaca ctgacaggac gctttttgat
     3061 agcgttctga atgcctttta taagcttctg tctgtcaaaa ggctcaaacg agccgtcacg
     3121 cttgtatacc ataagctgtg gcttttcgac cacctcataa gtagtgaaac gctttccgca
     3181 tttcggacac tcacggcggc gacgcttttt tccctcgctt ggacgtgaat ctattactct
     3241 tgtatcttca tagttgcaaa aaggacatct cattataaac actccttagt taagagaaaa
     3301 ttttgtgcgt ggtattgcat atattgtgct tttattaatt aattatacca tagattgtgg
     3361 acgattgcaa gagggtatat aaaaaaagtt gcggtaaagg gactcagcaa ttttccggca
     3421 gacaaaccat agcataattg taggggacgg cgccctcgac atcccctaca tgggcagtgc
     3481 aatatttatg cacggatatg gcag
//
LOCUS       DAWEMR010000235         3483 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_9266_length_3483_cov_8.094224, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000235 DAWEMR010000000
VERSION     DAWEMR010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9266_length_3483_cov_8.094224"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2782..2791
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          963 a          616 c          859 g         1035 t
ORIGIN      
        1 atgtctgtgt gcggagttgt ttgatacaac agggcattca gcctttgcat acttttcttc
       61 aagggctctt atctcgtctt tcttcatatc aactgcgcag aatacgaaat ctaccattga
      121 agcgatcttc tcaacgtcct cctcggcgtt cagaactgta agctcaccga tagcttccgg
      181 cagaggctct gaaagcttcc acttgcctgc aactgcgtcc ttgagctttt tgcctgctga
      241 acgtggtgaa gcggcaacgc atactacatt gaaccatggg tgtttgtcaa gaagcagaga
      301 aaatctctga cctaccattc ctgttgcacc gatgataccg acattgtact ttttcatatt
      361 aaaactcctt ttcctgtgcc atggtaacaa ttttctttgg cgttatacac taaaatatgc
      421 cgctgttcaa atgttatttt gcacaaaaca taaaaaataa acaaataata aaaaaactgg
      481 ttgaaaacca gttcatcata caatataata agaatgttga cataaaaatt tatgccgata
      541 gcactccacc atttgtaatg atgacaactg atagtctatt aaactaacag tcagcaggag
      601 tgttcccgat actccgcttc ggcagtttta cctttctcat ttaccatagg cgttgggacg
      661 ctgttgtgtg gggtcacacg aaaaatgata ctcatttcaa ctgcacctct atctgttctg
      721 tttaatgata acaaatattt agcgttctgt caatacatat agataaaaat attttaattt
      781 tctgtaaatt aataagttta tatgaaatta attagactta cagagcggtg acggctattg
      841 ggtcgggaat tttacatcgg gacgtcgggg gcgccgtccc ctacaaagtt attcatgtac
      901 attattatat tattataaat aggagatatt taaaatgctt gatatcaaca atatgaaaag
      961 atcggatttt tattatgacc tgccgcagga gcttattgca cagacccctg ttgagcctag
     1021 aaatgcttcg agaatgatga agataaacag gcagacggga gaggtggtac acgaccattt
     1081 ctataatcta tgcaattatc tcaaaaaagg tgacttgctt gtgcttaacg actcaaaggt
     1141 gcttcctgcg aggatatacg gcgagcgtga ggacacaggc tcgttcattg agtttttgct
     1201 tgtggagcag aaagagaaca tggtctggga gattctttgc agacccggca aaaaagccaa
     1261 aatcggcaca agatttgttt ttggcggtgg taagctgaag gcggagataa ttgatgtgtt
     1321 ggaggacgga aaccgtatcg cacgatttga gtgcgaggag aatttctttg ccacacttga
     1381 cgagataggt cagatgcctt tgccacctta tattactgaa aagctgaagg acaaggacag
     1441 ataccagacg gtttatgctc atgagcttgg ctcgtctgct gcgccgactg ccggtctgca
     1501 tttcactgag cagcagcttg aggacttaaa ggcaatgggt gtgaacattg cgtatgttac
     1561 tctgcacgtt ggtcttggca cgttcagacc tgttaaggag gacaaggtcc ttgaccataa
     1621 gatgcacagg gaacattatg agcttagccg tgaaactgca gacatgataa acgaaacgaa
     1681 gaaaaatggc ggacgtgtta ttgcggtggg tactacttca tgcaggacac tggagagcgt
     1741 ggcggcattc aatgacggaa agatagttcc atgtgatggt tacacggaga tatttattta
     1801 tcctggcttt gagttcaagg tgcttgacgg acttgttact aattttcacc tgcctgaaag
     1861 cacgcttata atgcttgttt ctgcatttat gggatacgag aacactatga acgcttataa
     1921 gatagcggtt gaagagaaat acagattttt cagctttggt gattgtatgc tcataatatc
     1981 tgagtgagga attaaggtat cgcctgcggc gatgatatct tatttcaaac tttgttttac
     2041 ggaggttttt tctatatgaa tgacgttaag tcaggggaga aaaagcggct ttatttatat
     2101 gataatctga aatttctgct tatagtgctt gtggtcttgg ggcatttgat agacgacagc
     2161 actgtcaagc tttttggtga cggcggcggt gaaacaggtg taccgcaggc aaaggttttt
     2221 agcggcgcat ttgtgtttct gtatgctttt catatgccat tgtttttatt catatcagga
     2281 ctgttcaaca gggggcatga tgacggcagg aaagtgcttg gaaagacgct tggatttgtt
     2341 gttatcggaa tgttaatgaa atgcctgaat tattggtcgc aggtgagatt tcagacggat
     2401 tttgacaagg aaaacagtga tggcgagata ttctttgacc ttttaggcgg cgacggtgta
     2461 tactggtata tgttcgcaat ggcggcgttt atggggcttt gctatctgtt aagaaacagc
     2521 agaccttggg cggtgcttac ggcggctatg gtgacggcgc ttgcggcagg gtatgaccct
     2581 agcgtgggcg atgaatatga gctatccagg atagtggtgt ttttcccgtt ttactactgc
     2641 ggatatgtgc ttgaccctga aaaggttgct gattttgtga agaagtggta tgtaagggtg
     2701 ctgtccttgg gagtgatagg catttgggct tatttctgct ttggaaaaac aaagcttgtt
     2761 tatccgctga gaatgcttct tnnnnnnnnn ngattgcaca tttttgagca ggcttcttgt
     2821 tatgggtatc tcggcactgc tttgtttggc ggtgcttggt attgggcttg atgtgaagat
     2881 tccactgata acaaagtgcg gctccaggac tttgcaggtg tatttctggc acagaactat
     2941 agtttatatg ctcacatact atggttatca ggcatatctt gcaaaggctt ttcctgaacg
     3001 ttgggagctt tatttgtcac ttacagcgat acctattgta ttggtgcttt gcataaaaat
     3061 atttggtgtg cctcttgata tggtgcttaa aggaataagg ggcaggaatg atattataaa
     3121 ggagaacgga aatggaaaat aactttttta agagcgttat tgacgcagac gatgcacctg
     3181 tggttatttg cgaccttgag catacgatag tatatatgaa cccgacagcg gtcaaaagat
     3241 atgaaaagcg tggaggagcg gctcttgtgg gcaggtccat aaagggctgt cacaacgggg
     3301 attctaatga caagatagag caggtcattg catggttcaa aaaggacaag ggtaacaaca
     3361 aggtgtatac tttccgcaac gaaaaggaaa ataaggacgt ttacatgata gctctcagaa
     3421 atgatgaggg agagcttatt ggatattatg aaaagcatga gtacagaaat attgaaacta
     3481 tga
//
LOCUS       DAWEMR010000236         3460 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_9338_length_3460_cov_13.298678, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000236 DAWEMR010000000
VERSION     DAWEMR010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9338_length_3460_cov_13.298678"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1035 a          690 c          796 g          939 t
ORIGIN      
        1 acacgcagcc gtgaatggcg ttttcgataa gatttccgaa aagcacagag aggtcggtta
       61 tgtctatttt tatatcatct gggatgtcag cgctcacctt gtattcaatg ccgctgcttt
      121 ccgctatttg agcaaaatat gcaagaagtg cgcttacaga tgtgtttttg cagtaaatgt
      181 gttctgatat atcgggcata gaatccctga aagaattgag atattccgaa agcttatcgt
      241 attcgccctt tttcagaagc tcctgcataa gagcgatatg atgtctgaca tcatgcttat
      301 agcgtcttgt ttcttttatc ttatcctgca aatagtcata ttgcaggttc tgcatttcaa
      361 gcaggtggtt attttcctga agccgcagat ttttctgctg ttcgatgacc agccgtgcca
      421 cgatgtaata caccagaaac gcccctgcgt ttataaagaa cagagctatg gtgttctttg
      481 gctccagagc aagctctaag ctgcttttcg tagagttgcc gtagagagta tagtaccata
      541 ttatatagaa cgtaaatggg ataagccaaa gatagcgcca ctccagacct gacggctctt
      601 tttccaccgc aggtgtgtag acctttttca tgtagataaa aagcggcccc cataccacgg
      661 cttctgtcat aagcatacaa agggaaaacg accagcggta tgattcatag gcaagtgacg
      721 ggaacagaat gccctccaga cattttgagg taatgacgat aaagcttgcg ttatttgaaa
      781 gcagaagcag tgtgaagcct gttttgccga ccttgacctt cacagcgaaa aagtaaaaag
      841 ccgcatagag cagggtgctg actgcactta gcagacctgc atttgtgctg aaaaatgccg
      901 cctgccagcc taaaaagatc tgcaccagtg aaagagccac aacaagtccc acggtagctg
      961 ttttggaaaa gcgaagccta tgccggaagg gataaagggc aagcaccata agtggcagaa
     1021 aattcagaag ggaatatatg cccacttcca caaggcggta taaaggaggc attactgctc
     1081 accgcctttt cgcagatcac tgaagcgaaa ctgagcgaag gcgtctaatg tttctctgga
     1141 ttttcggcgg cttatgggga tatgtgtgcc gtcgctcatt atgaaaaggt cgcttttttg
     1201 ctgggctatc tcatagaaat tcacgatgac gcctttggtg gaggtgtaaa agtatgaatg
     1261 actgcaaaaa atgctctcga tctcagcgaa agaatttctt gaaactatgc tttcaccgtc
     1321 tgtgcaatgg atagtcacga tatgtgacgc acagtctatg tagataacat ttctgagtac
     1381 cacgttctga ccgtcgggaa gcttaacgga acgcatacga tcaatttcat cagaaccgat
     1441 ccggtcaagc atggctttta ctttgtcagc ctgaaagggc ttgcaaagat agtagcaggc
     1501 attgaggtcg tagctttcgc ttgcaaactc gttgcttgag gtgccgaatg cgatatgcac
     1561 gtttttgtct gtttcacgaa gcttttctgc gacctctatc cctgtcattt tccccatgaa
     1621 tatatcaagc accaccagat cataagctcc catctgccaa catgataaaa agctttctcc
     1681 gcttgaaaac gtatctatct gacagctgtg agcggtttgt tcggaaatat attcatgaag
     1741 aagtgcaagg tcgtttttat tatcgtctac caatgccact ctcatactgt aaaaatcctt
     1801 tcttatacat gatttcttaa tatataataa cacacatcaa agcaaaattc aagcattatt
     1861 catttatttt gttaccgctt gtgcagattt ttgaccgttt gtgccaaatc tattgacaaa
     1921 gtgtgtaggc cgtgttaaaa taatcgtgat aaattttgtt ctgagtaaaa aagaaatatc
     1981 aacccagaaa aatataatgg aggaattaga atgaaaaaga aaattgtaag ccttttggtg
     2041 acgggctgtc ttttgtttgt gccgacaacg gcatttgcag acgacaataa gacggtaata
     2101 aaaactgttg acgacctgtt ggcttttgga aaagcagtaa acgatggcga ttttaacggc
     2161 aagacagacg ctgttgtagt tctcaataag gatcttgact taacgggcat tgactggaca
     2221 cctattggaa atgtttttaa cgcaaagggt gaacttcaga attatttcag cggtaaattt
     2281 tacggaaacg gtcatacgat atcaaatatt gactttacac ctatctatgg caaagacttg
     2341 ttggtcggtt tctttggcga tattgaaggt gcagaggtat caggtctgac tatagaggga
     2401 agtattgatg tgacaaatac tgataacgac tatacttttt atggcactat tgcaggattt
     2461 gcaggtgatt gtactattac tgattgtgtg tcaaacgtat ctttcaacaa taacggcaaa
     2521 tatgtttatg gcttaatggg catggtaggt caggcagacg gcacaacttt tgagtattgt
     2581 gagaacacag cagacataac tatctcaggc gacagcggaa gtctttatgt gggcggtata
     2641 gttggatatg cacagaatgg aacggaagtc cgctattgtt caagcacagg cgatatggta
     2701 tatgctgcac cagacgccgg cggtatcgta ggatgtttgt atggcgattc caaggtcata
     2761 aacagctatg ttacaggaaa actcacgccg gttggcaacg gaaccacaga tgtaggcggt
     2821 atcgttggtt cagtagccgg cggttctgta agtgattgct attttgcagg agaaattgac
     2881 ctttcacagt attcagccag aaagccatat acacgtttcg gcggaatagt gggaaaagat
     2941 tcctcgccta cacctgactt taaaaacaac tatttcacag aaacagaaga tgttgaagct
     3001 tgcggttcaa ataaagaagc aggcaaggct aaagcatatg actatatgac aacaaaagaa
     3061 ttttatgacg agctgacagc cggcggtgca aagtatcagt atgttgaggg aaaaacgcct
     3121 gtcctgccaa ctaaggaata tgcagttgat tttgaagtaa cacctgctga cctgaaaaat
     3181 gttgtaataa aggtagacgg aaaagagata acaagcaata ctgcaatgct tacagcaggc
     3241 acttacacag tagaggtaac tgctgacgat tgcgagcctc tcagcaagga aataactatt
     3301 tcagcagata tcgcaacaca tacacaggct tttgaacttg catacaagtc agcagactac
     3361 acagagcttg acaaggcaga gaaagccgca aaggctctca acaaagacga ttatgaggac
     3421 ttctcagaag tcgaaaaggc tcttgccgct atcgacagaa
//
LOCUS       DAWEMR010000237         3372 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_9586_length_3372_cov_10.415134, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000237 DAWEMR010000000
VERSION     DAWEMR010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9586_length_3372_cov_10.415134"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          931 a          755 c          593 g         1093 t
ORIGIN      
        1 acgcagatga taccgggtct gacgatattg ctgttgagct tatggacgtt gcagacaact
       61 gagcttcgca ggaagatata ctttactaag atattgtgat ttaatacagc aaaaagaggc
      121 agagaaaccc attgatcggg tttctctgtt tttttatttt tgctaaagca gacaaccaaa
      181 caccgccacc tcctgtcccc caagagagcg gcggtatcta cactacgcta cttttcagta
      241 agatattcat ttctgtcaaa caactgctca attgaaagat ttttaaacct cggaatgttg
      301 tatccaaacg gcgagttgag agcctttaca aagtctttcg tatagccgtg attttttaaa
      361 agatcgtcct catactttat tttctcttct tgctttttgt catcttccct tttcgtgata
      421 cagcgctgca taacataatg ataaatggag aagtaagaat ccactgtacg aatatctcct
      481 atgtattctt tcagttcacg aactacagta agatgagcaa tacagttgcg gtattttctt
      541 gtcatgctgc tgtctgcatt attgatatca acttcaacgc acttgcgcag ccgcttgttc
      601 tttttcaaga acaaattcgg cgactcatca cgatcatcac aaagttcaca aagcgtctgt
      661 gaaagtatcc tgtagtcatt tttcaagttc tttgaagcca actcaggaat tatctcctta
      721 tacagcccaa aatcacgttc aaggcagtgt atcgcaataa cataccttgc attgacattt
      781 acaagatttt tcaccagcag atacatgacc gtaagataaa gcccgataac agcctttgcc
      841 ctctccttag ccacgttttc tctgcccttt gcctgctgtt tcacattttt gaaatcatca
      901 aagctgatgt tctttatcat tctcgcaagc tcactgcgct ttacttccaa agaactgttc
      961 atgtcaggaa attccacaca actcttgtaa taacgctcta tctgcgtgtc agggataccc
     1021 ccaagaacaa acatgacaac tttctcattc tcagccactt ctcttatctt ctgagcgttc
     1081 gcatacttga taaggtatac aaaccgagag gactcgataa cattgtttgt tatgaaattt
     1141 ctcagaccat gtatgccctt gcctttttct ttaagcttta aaatctcgct tatcctatcg
     1201 tccgtgatct tgtcgtctat accgagtata gtcagtgcgt cacggaacat cgtaagcttc
     1261 gctgaagccg caggctttct catggaagca atgttcttta cgataaaaag ctcgttggtt
     1321 atcctctggc tgtcattgaa cagcttgtaa cccgccgtaa gctcacactc aacatcaaca
     1381 gcagagcttt tcattatctt caaaaactcc ttgatgttat caaacttgct gataagcgtt
     1441 gtaagaagat cgtttatctc cttgccgtca agaaaatatg tgagcatata tatcattttg
     1501 gagaaataca aaaggtcaga cgcattcttc ttttcactgt caagaatttt ctcatcaaaa
     1561 tccatgtcag ccttgccaag ctccttgata acgtcaccgt tcatgtggtc ggcgatattt
     1621 tcaaaatcat tcctgaattt gccccaaagc ttttccgctt catcagcata tatccactct
     1681 ttttcatcat cggtcattga aaaacgcagt ttgcgcacaa gaacttcgcc tgcggcaacg
     1741 tcatttctgt agtagttgca gaaaagcaga aaatccataa gcttgtacat ctttgagcgc
     1801 acagagtcat attgcttgtc cttaaatctg aagccgtatt cgtccagcat tttctcacga
     1861 agctttttga tagaaaaacc gagatttttc tgagatttaa gcacaatgaa atcataataa
     1921 aggcgtatga tatcatcagc ctcatagcct ttcatcatat caataagcaa gctgatattg
     1981 accttgttgc cctggacaaa gcccttattt atagaatcaa atctctcatc tacaagatag
     2041 tcaagagtgt ctctgtattc ggaatcaata atgtcaataa agctgtaaag gtactcatca
     2101 agctcatttg acttatcatg gaaaacactc tgccttatct gccccacaat tgcaagcata
     2161 tgataaacac gctttttgta tgcctccgaa actcttttat ctttcgtctt aggctcttca
     2221 agaccaaaat agccaagacg cttagttttc agcaggtcat taaacttgct aaggcttttc
     2281 ttgatattac cctttacctt atcggaaaga ttacttttgt cagggtgagt aaaaatataa
     2341 taagtatttt gggcagaaag ataccccata aaatcgtcat gactttcatc gtcgccttcc
     2401 ccgagcatat tgttcagggc ataaacgata ttcgtcacat acaccgcaag tatcttttcg
     2461 atatccagaa tgttgtaaat aagctggata tgtatattat catcaaaagt tttgccgaaa
     2521 aagcgctttt caagctccga tttaagcccc agcatatccc ctcttacagg cgagctttca
     2581 ccgcttctgt gagtggggtt tgaagtattt atctccactc cgctctcaaa gccatgcttg
     2641 ctggaaaaag tgatgtttac ttcgtttaca ccacaaagct tgatattgct gctgccctta
     2701 gaggaaagct gagtttggtt gtagacatta ttatccacct tatccacctc atattcaagc
     2761 acagcagaat tgcccttgcc aaaagaggtt atatacattt tattttcgct gacaagaata
     2821 gacttcattc ctgccgcctt gacggaggat tttttcttct ctgccgcagg tgcaatgacc
     2881 tctgcagcag gcatggcagc gatcgcagga gcagcgttat tatttatctc agccgctttg
     2941 agctgtctgg cttttttctg agcctcacgc agctctctag gcttcatttt attctttttt
     3001 gccataaata ttactcctta tattagttat cccttagcgc attgagcgtt aagggatttt
     3061 tttatttgtt gatttcgatg ttaaactttg ccaattggcg caaattcaca ccagtatagt
     3121 agcaaatttt aaaatttatt ggaggtatta tcagttttag accagtgcaa aatcgcacta
     3181 gtatagtagc attcccttct ttttctgagg tgcgatgcgt tttagactag tgcaaattcg
     3241 caccagtgta gtagcttttt gccttgtttg tcaaggttct tttagtttta gactagtgca
     3301 aattcgcact agtgtagtag attgaaactt cttgcgaaaa taattcagaa agttttagac
     3361 tagtgcaaat tt
//
LOCUS       DAWEMR010000238         3360 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_9614_length_3360_cov_10.246899, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000238 DAWEMR010000000
VERSION     DAWEMR010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9614_length_3360_cov_10.246899"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2674..2683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          807 a          780 c          687 g         1076 t
ORIGIN      
        1 gctttttggc aagatcgcag ccttttttct tggggacttt ttttacagcc cctttatatt
       61 ttgtttactt gtttttcttt ttgcgctttt tatcctctaa aggagtcatg ctctttgcac
      121 agatcgccat tgttgaagcg ttgtgcagaa gcgctgaggc tgacggctgt aaaagcccga
      181 aagctcctga caacagaagc actgaattga acagcagtat aaatctgtag ttactgttta
      241 ttcggtgcat tagcttctcg ctaagctgtc ttatagacgc aagctgtgta aggtctgcac
      301 cacgaactgt gatatcggca gtttcctttg cgatatcgga agcatcgctc attgcaacag
      361 aaacgttcgc cgccgcaagg gctggagcgt cgtttattcc gtcgcctacc attattactc
      421 tgtgtccatg ctctttgagc tgttgaacat atctgtgctt gtcctcagga agaacctgcg
      481 ccctgaaatc tgatatgcca agcttctgtg cagttatctc ggcagccttt gcgctgtcgc
      541 ctgtgagcat cacgatattt gtgatgcctg cttctttgag ctgtttaaca gcttcctcag
      601 cttcaggtct tggcgggtcg cttatgcaga gcgcacctgt gagcttgtcg cctacagcaa
      661 gataaaccac tgagcaagct cccgactttt cgtcgatctc agcctgctgt tcgtctgtta
      721 tctctatgcc ctcgtcctcg cagacaaaat gcctgcttcc tatgatagca cgtttgccgt
      781 gaagcgttgt tgaaattccg tgggcaacta tatactgcac ctcagcgtgt tcctcggcat
      841 ggtcgatatc tctatcggcc gcacccttta ctatagctct cgctacgctg tgagggaagt
      901 gctcttcaag gcaagccgca attttcagcg tttccgcctc ggagtaatcc ccgagggcta
      961 ttaccttttc aagcacaggc tcagcgtttg taagagtgcc ggtcttgtca aaaacgatag
     1021 aatcggcaag tgcgtattct tcaagatatt ttccgccctt taccgtaacg tcatcatcag
     1081 cggcttcttt aatagcggat ataacggcta taggtgttga aagcttgata gcgcaggaat
     1141 agtccaccat aagaacggaa actgcttttg tgacatttct agtaaacagc agtgtaagac
     1201 caaaggcaag gaagctgtac ggcacgatag agtccgcaag acgctccgct ttgctctgca
     1261 cccctgcttt aaggttttct gagttgtcga taagctcgat tatcttgctt atctttgtgt
     1321 cggaagaaag cttgcgaact tttatcacaa ctgagccttc ctcgacaact gttcccgcaa
     1381 atacgcttgc tcctgcgctc ttcatgaccg caagaggctc gcctgtcatg gaagcttcat
     1441 ttatgtaagc ttcgccctcg gttatctgac cgtcaacagg gataacgctt cctgtgcgaa
     1501 ctcttatgca atcgtccacc tgaagctgtg acattggtat aagagtatca gcgtccctgc
     1561 ctgccagcca tacctgatct gcctttatcg ccaagctgtc agtaagagcg gctcttgttc
     1621 tcgctcttgt gtaatcctcc agcaagcctg atacagaaag caggaacatg acagtgccag
     1681 ccgtattgta atttttctgg ataagacaag cgcttattga cgctccgtca agcacctcaa
     1741 ctgtaagctt gccgtcaaac agtgcgctta cgccctttgc tatgtattta agtccgctgt
     1801 acacgataag tgctgcgcct ataggtgcag gcagaatagc ttttcttata tatctttgca
     1861 tcacaagctt tgcaaggtca gatttgaatg ttctgtctat ctcctcggtg tcatactgat
     1921 tgtcgctgac aggtgccagg tcgcttcctc tgagctttct tacaagttcg acagcttcgg
     1981 ctctgtgacc ctctttatag cgtataagta ttccgccgtt ctcatagtga gcctcgcagg
     2041 cgatcgccca cgggtttgac ataacaagct tatgtatcga ccgttcaagt tcttttgtga
     2101 aagcgttgct accacagcga aaacgtatgc ggcagggctt gtcataaacg atctgagctt
     2161 tcattattca gttacttctt cctcgtcatc tatccctgcc tcgaccttag cgtcatagca
     2221 gatgtcagat gcttcgtcct tcatgcttct cagtgctgac tgagcgtcgc tcttgagctt
     2281 cattcccttt gcaagacctg taacggcaag ctctcttgtt ttcttagcct ttacgacttt
     2341 cttgccaaga atgactgcgg ctgctccgcc tactgcacac cagaatttct cgtctttaaa
     2401 acattttaac ataattatta cctcctgttt tcgtctctaa agatcagtaa gaactaatta
     2461 tttctgtata tacagaaact ctgtcaaaag actagctttt tacaagtctt atgacagatc
     2521 tttaccacgt ttttattata actccaagat gtttattagt caagtcaaac cgacaaattt
     2581 aaaaattatt taaaactatg tataatcttt tgccgtaagt taaattatca tcgtttttct
     2641 aacttttcat ttttgggtga agctttagga taannnnnnn nnngtttatg atatcttatc
     2701 agtaaaccgc aagaatgaac attacaacga atagataata attgacaaaa accataaatt
     2761 cgggctttta tttatatgta agttatgcac acaaccaaaa atattataac tttttgttaa
     2821 atcggttgaa gccataacag agttatgtta aaatattacc ataacaatac ggggaggtct
     2881 gcgttgtggg ttatattatc cattatgata tatgtgccct tgtgatatat gctatcacag
     2941 gagttgtatt ctatttcaaa aagcgtattc cgtgcaacaa gaataaggtc tttggcattc
     3001 tcgtgggcga ctcggctttg tcaactgtgt tggatatttt aagtgtgttt tctaccggcg
     3061 aatggggcat agggcttaaa atcttcttcc atacggctta ttattttaca cataatctta
     3121 ttccgtttct atttgtgata tatatacttt tcctgacgga cggctataaa gagatgtctg
     3181 ctcttttcaa aagcttttta tatacaccga tgatcgttga tcttttgctt gtgattacga
     3241 cgcctgcaac acattttatc atctatgtgg atagtcaagg tggttatcat cgtggcactc
     3301 ttcagccatt tacatacatt gtggcgattt attatcttat atttggcatc gtttacgcaa
//
LOCUS       DAWEMR010000239         3360 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_9615_length_3360_cov_8.754009, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000239 DAWEMR010000000
VERSION     DAWEMR010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9615_length_3360_cov_8.754009"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          930 a          714 c          573 g         1143 t
ORIGIN      
        1 tgtgagcttt atctcggctg tgttgtcgcc tatggtctgt actgtgccga aaaagttggg
       61 gaggtcacgg tttagctgtt cttcatagtt tttctctcca ttttcgtcat attcggcaac
      121 atacagttga ttaaggataa aattattgtc cttgaaaaga taccccacat atacaacatc
      181 atcgacttca tcgggatagt ttatttcctg atatactata agatcgttca ggtcggcttt
      241 taatgttctt tgtggatctg taaattcaca ggcagatccg cccgttcttg tcatagtaca
      301 aatgccgccg ctgtttgaaa aaactttgcc gttttgacct ataacatcac acagcttgat
      361 cttagttatt ccatcatcaa cggtaagaac agcagtgacc ttttcaccaa gcttattgtt
      421 tatgatctgt tctgagtgga gctttgatcc aggataataa actatatcgt ctttaaagtt
      481 atctcttgaa agcggcaatg tatagctttt gccctcatat gtgaatgtaa gctgttcatt
      541 ctcatatttt tctgtttcca cagttatgtt ctcaaattcc gaacttggct ttgacacggt
      601 gctctccggc atatttgaca cgctgtcaac agcagagaga tccgactgtg gtttattttc
      661 cccacaggaa gtcagaaaaa taacagcaga aaacacgata cacgaaaacg tcataaactt
      721 cttttgtttc atgatctacc tcactgcata agcatattta tatcaaactc ataatcaaga
      781 tcgccgcttt cagcgtcaag ataaataaat cgctgaatat tgcctgccga tgaatattca
      841 tcacctactg gcatttcatt ttcgggaaca tccataacaa actcccagac caaatggctg
      901 tcaaatttta tgcctgcatc gtagcagctt tgaccgtttt tattatcgtt aagaggggtg
      961 agtgtgtatt taaggtcaat ggcacttatc tccattttct cattaaaatc tgtaaaagta
     1021 ttttcaataa accccattgc acttgacagg gaaattatct tatcaacaga tctttctttt
     1081 tttggtatca agataccgtt gccgttagta aggctgccta tctcgtcgct tttaaacatc
     1141 tgcatatata tgccctgcgt gatatatttc attttcgaca tactttcatc agacgtatct
     1201 gtcatttgca caagatcgtc aagcaatacg ccgttgtatt ttttagacgc atggatatca
     1261 aaagagtaaa tgccctgatc gctttgtctt acaattatag ttttaacggc tatctccaag
     1321 tcaggttcaa gatcggcata gttcttatcg agccattcct gagcaaaatc agctgcctgc
     1381 ttgatcgtga ctgaagttcc gtccagttca tagctgtctg aaagatcgtc atctctgtca
     1441 atatgataga tcttgactct gtttccgccg ttaaagagat catcaaaagc tgagggcttg
     1501 gcaaaacaga taaagccgtt atttccaacg cagccataca ttttcccgtc atcgtcgcta
     1561 aagctgtaac ttaccattcc gtcgccggtt tcatcatgaa ctgtattctc acggtcgaga
     1621 acatccttgc caaaaattcg ggtcattatc ttttcgtaat tttcctcata atccgatatc
     1681 tgagtaaaat cgacttcgca gtaatcttcg ggcaatttta cactgatatt ttctctcaga
     1741 tcaaattgag tatagctttt atcaagagca ttgtctatgt catcactgag ttcgttggaa
     1801 gatatgtact gcatagctgt gatctcattt ctctctttac tgtctttctc ggcagaggat
     1861 cttacgctgt caggtgtctg cgcacagcct gcaagaacga cagccaccgc tgcggcaagt
     1921 aattttagat ttttcataag caacttcctt cctcatatgt gataacatca gtaagtcaat
     1981 ggtaattttc gcttttgctc aggtcacatt ttattgtgtt atacgtttcc tatttgatta
     2041 ttaacaaaag aaacgttcat atcgttgcaa tttatggttg cataaatttc cttgttcgga
     2101 gtttcgtcaa agtaaaacac ccagcatgga acagcttcat atatgtcata cgagcagtaa
     2161 gttgcccagg gagctttttc acggtctgtt tcttcatctc ctgtgctttt ctcaatatta
     2221 ccttttttaa tcatacagta ttcaatagct acacgcttga cgttcaactt catatttgtt
     2281 gctaaagtat cacttaaaca tttagcggca gaaacaggat cggatgtact cgtcaattca
     2341 ccgtcgattt tatattcttc aaagtaggag ttcgtgacaa aattattgac ctcgtttccg
     2401 cagatatatg cgaaagcgca ggacacgatc atattttcgt caatgctatc atagcctgca
     2461 tattccaaaa tatttacgtc agatacggac tgagtataat ttgccatata aaaatagccg
     2521 tccgaacatt catacaagct tattctagat actgacagat cattgcgata attgcagaaa
     2581 tttgtaaaat catcagcaaa atcctgtgct aactttacag cctcaggcac agatagctct
     2641 ccttcactgc caacaagctt atatttttct gagctttcca cagcttcgga gtatggaaac
     2701 gtcttgacga attcggattc attgtaatat ttttgcacat caaaaacctt ttgatacgaa
     2761 aaaaatccgg tacatccgat cgacaattgc agaccctttt ctttatcctc atagctcgga
     2821 cctgttgggt aagtagtatc gtcattcgta atatttgctt cattgaaatc ttcatcataa
     2881 tgtgtgaata tttccttgta attatcctca aaacctgtag gacatataaa atctcctgtt
     2941 tttatcttct gcggaatatc tatatcaagg atatcactga aattcatatt gtcacattca
     3001 agctttttaa gttcatctat atactccgat ttctttttac tcatttcatc cagcgaagtg
     3061 agagcatatc cctgagtcgc agtgttttca ttttttgatc ctttcttaac actttcaggt
     3121 gtctctgcac aagatgacaa gaataacagc gacaggcata aaatcgtact tgttattttt
     3181 aaatgtagct tcattttgtt gctcctttga tttcttataa tacaaggcaa taccgcacaa
     3241 agactgcttg acggtgttat gcggtatcgc attatatttt tatgctgtta tccatacttt
     3301 atcaactccg atctatattg taaaattaca aatttatatc acctctgctt tgcgaataga
//
LOCUS       DAWEMR010000240         3284 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_9866_length_3284_cov_10.657789, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000240 DAWEMR010000000
VERSION     DAWEMR010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9866_length_3284_cov_10.657789"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1080 a          553 c          792 g          859 t
ORIGIN      
        1 caaatattgt gtatggcttg ctgaatttga caagtcaaag ccttcatacg gtggtagcta
       61 tggtatgtgg cagtacacaa gtaaaggctc ggtttcaggt gtaaatggaa actgtgattg
      121 caattatgcc tataaagatt ttaccgcaat tataaagaaa aagggtctta atggttttaa
      181 aaagcaaaaa aataatgaac ttccgatact tgaaaagtct ggctataaaa aaggtaataa
      241 gaccagtggt gttcttgctc tgaaagaaat gctcatcata gccaaggcaa gaaaacttca
      301 caatgtcaca cttgatgaaa atagtatatt tggcgagggt actgaaaagg ctgttaatgc
      361 tctgctgaaa aagtggggtt ataagcagac tagcattgca ggcgagaagt ttatcaagaa
      421 gctggcaagt gctattaaat aatactaatt atttttgttt ttaaagggcg aggtaacaca
      481 gcttcgccct tgttatattt tatttatacg aaaggaagat gatttatggc gtattgtgct
      541 acgaacggaa acttgtacga aaatggaaaa gcttttgagc tgaaagttgg cattggtgct
      601 gattttaaag tacaggcttc gggaactggt agttttcagg ttgtaggaaa actgactcag
      661 aatggtgcag aggaagtgct tatgatggtt gatctgagcg acttctcaac ggttgatacg
      721 attacaacgg aaaatgttta cgcaggagat gttagtggtt actatagtgt aactgttaaa
      781 aatgtcaagg gtgtaaacaa aatttgggga actataacat attaaggagg tggatttatg
      841 gctacagata ttattgctag aggtatggcg gttaatgcta aaaagtctgt cactgaatta
      901 ggcaacaagg ttgaaagcga aaagtggatt ggcacaaaag ccgagtggga agccgttgat
      961 aaatccacta taaaagacgg cacaattgta tatatcactg atgataaaac ggtgatttta
     1021 tacgataagg cggaaatgga aaagatagcc gcacaggttg ccacagaccg caaagccgct
     1081 gaaaccgctg cacagacggc acaggcggtg gctgatagtt tgcctgaaga ttatgttacg
     1141 gcggtttcaa aaattgccaa aaatacggct gagataggac gtgtgaagct gaccgacaag
     1201 gaactgcaac gtagggtaaa tgcactgtat tccatcggtc agggtatcac acacaaattt
     1261 gaaactgaca gtgatacggc atatcagaaa actattccta cggggacaaa gctgatgtcg
     1321 gttaagaatg ttggtggtag gtctatcgtg tttaatcagg ttgttaacct taacaatttt
     1381 cgcccaaact atacagtgaa cggtgttaca tttacaaagc ttgacaatgg caagtttgta
     1441 gcaaacggca ctgcaactgg cggagatgcc tacttctcgg atagctttat tccgattaaa
     1501 ggtcatgtgt atctggaaaa atcctgccct aaaggtggct cagcagagac atatcgttca
     1561 tacattacag gtagtggtgt tgtgatggac accaattatg gaagtggcgt aatagcaccc
     1621 attaatgtag acgcccgtgt atatatagtt ccgctgatgg ttaagtcagg agcaactgtc
     1681 aataatttgg tagtctaccc acagatttat gacctcaccg caatgtttgg ttcaggcaac
     1741 gaacctgcga gcgttgaaga atttgagaaa attttccctg ctgcctatta tccgtataat
     1801 gccggcgaaa tagtcagcac tggggtgaca gaggtcgctg tgggtgatac cgcacataaa
     1861 atccccgaag caatcaaggc actgcctggc tatggttggt cagcaggaac ggcacgaaac
     1921 tatgtggact atgaaaataa acgatacgtt cagtgtgtga acagcgttga tttggggacg
     1981 ctggattgga cgtctagatt atcgacagtc gacaacagta tatacgtttt tatttccaat
     2041 gatttaaacg tgaacaaccg aggtaataat ggattgtgta gccattatga ttttgttaca
     2101 tctgatattg acgcaaccat agcaaaaaaa gataaatttt tctattataa tccactgaac
     2161 atcgcattta ttgataaatc ctacaccgat gccgccgcat ttaaacaggc aatgcagggt
     2221 gttatcctgt actatgaatt agcgaaccct atcgtaaccg atatttcaac cctaatacca
     2281 gatgatttcc tgcgaaacat tgaggtagaa gcaggcggta gcataacgtt caaaaacagc
     2341 aatggtgaca gctattgcat acccgtgcca tcagaggaag agtacgttgt aaaactaagt
     2401 gaagtaggag gtagcgtatg acggatttac aaaagaaaat ggctgacaag ctagggttga
     2461 cccctgatga ttttcagccg aaaaaagcca cgaaagttga cgagctagaa gcacaggtgc
     2521 tatatactgc gctgatgaca gacacactaa tcgaggaggg caaggaagat gtatagaaaa
     2581 gtcaagaggt tgtacgattt agggctgtac accactgagc aggtcaagga ctttgccgac
     2641 agggggaaga taacccctga gcagtacgag aaaatcactg gggaaaagta tgaaagtgag
     2701 gtagcgaggg tggtggaaag accaaatgag cgtaagtata tataataaaa ctgataacaa
     2761 gcttagttca ctagcaaacc aaacggagct tatggacagt gacagtacgg cagatattac
     2821 aagccaaata gaaaatttga ttacctcggt taaaagaaac acaaatgaaa tatctactct
     2881 gagtggaagt tgtgttcgca tgggggaatt aaatcgtaat gctcataccg taggtggaac
     2941 atggaattgt aatgatccag ataatataaa tgggcttctc ggtcaaataa atcgtggcga
     3001 tatttacgaa ataggtcttg gtacagaact aaaactaaaa ggaactattg aaaatgttcc
     3061 ttgtatcgtt aatggcgaag aaagtacaaa aacggtagag tatgatactt attttgtatg
     3121 tgtagctgtg gattttctta gaactacaaa agcttcaagc gggaaacggt catatacatt
     3181 tatgcccttt ggttcgccaa taggaacaaa tgtcattgat aacgctacag gtttaggtga
     3241 tgttcacgca tactctcaaa cattcattca gcaaaaggtt atgc
//
LOCUS       DAWEMR010000241         3264 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_9925_length_3264_cov_13.577750, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000241 DAWEMR010000000
VERSION     DAWEMR010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9925_length_3264_cov_13.577750"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          945 a          657 c          735 g          927 t
ORIGIN      
        1 acgctgtttg tatgtagggg cgaacagtgt tcgcccgttg atttgacatt tttgcaacag
       61 actggctcat gtgcttattg catatgagct tttttattca aaatcgttct aaagactgtt
      121 taaaatgttg atttttatat gtagctgtgt tataatgaat tgtgtatggt tttttaccaa
      181 tatattatca gtaaagaaaa agaggacaag acaatgttta agaaaaacgg acttctaaaa
      241 agccctgaca gcaagccaaa agaaaagtat ctgctgctgt ttgcggtggc ttttatcggc
      301 atgatgatct cattcatacc gtcaatgata gtgaacaagg gcatctttct ctactatggc
      361 gacttcaact cacagcagat gatgttctat cagcactgtc atgacgctgt tcgcagcgga
      421 aacatgggct gggattgggg aacggactta ggctcgagct ttataggctc atatgctttc
      481 tatctccttg gctcgccatt tttctggctc actacacttt tcccgtcagg tgcagtgcct
      541 tatctgatac catggctcct tgcgctgaaa acggctatcg ccgcaataac agcctacgct
      601 tacatcagat tttttgttga caacacaagc gcctgcttta taggcggtct gctttacgct
      661 ttctcaggct tccagaccta caacgttttc ttcaaccact tccacgacgc aactgcgttc
      721 ttccctctca tgcttttagg atttgaactg ttggttcaaa agaacaagaa gggtccgttt
      781 gcggtagcgg tagctatatg tgcgaccata agctatttct tctttgcctg cgaggtcgta
      841 ttcgttatta tctacttctt cctgagatgc cttgacaagg actttgaaat tgacatgaag
      901 aagttcggtc tgcttatcct tgaggctgtt atcggtgtga tgatatccgc tgttatcttc
      961 ctgccgtcag cgctgagcgt aatgggcaac taccgtgtaa acgaaagact ttatggactt
     1021 gaccttgtta tatacggtga aaatgtaaga ataccaagaa ttatccaatc gttctttatg
     1081 ctttccgata tgcctgcaag aataaatatc cttaactcag acaaggcaag atgggcttca
     1141 atggcaggct atctgcctat gttctcaatg gcaggcgtta tagcgtttat gagaacgaga
     1201 aagaaaggca aagacagttg gcttacaaag gctattatcg tgttcggcat aatggcttgc
     1261 gtgcctatat tgaactcctc tttcgtcctt ttcaatgcgt catattatgc aagatggtat
     1321 tatatgccta tactgcttat gtgccttatg acagcaaagg tcatcgcaga aaacaaggac
     1381 gatctgaaaa agggatttgt gcctgtttgt gtagtatgtg cgatatttat tggcatcggt
     1441 ctgcttccga caagcgaaaa cggcgttgta tcctatggca aagtccctga atatattgaa
     1501 ctttactaca ttcaggctat cgttaccata gtaatgataa taatgcttgg cacgcttata
     1561 tatgtggtcg cagagaagaa gctcgactac atgaagatcg cttctgcaat gacggcggtt
     1621 gcggtggtca tctgcatgac ttcttctatc gtatacggcg taagccaggg tactgacaac
     1681 accgactaca tcaacagagc aataaacggt ggcgacaaca tcaacatgga taagctggag
     1741 tctggatcag agaactacaa tgctgacaat aatttctaca gaatagatac ctcagaaaat
     1801 gttgacaact ggtgtatgtt ctggaagctt tcatcaatga gatgcttcca cagcgttgtt
     1861 tcaacatcta tcatggattt ctacagctct atcggacaaa cgagagatgt tgcatcaaga
     1921 atggaaacta aggtctatcc gctaagaagc ctgttttccg taaaatacta tttcaatgaa
     1981 cttactgaaa gtgagagaaa aggtatcaca gaggtatcga agcctgaaac tcttgcaaat
     2041 ctctatggct ttgaatatgt tgatactcag aacggcttca acatttacga aaacaaatat
     2101 tatgtaccaa tgggcttcac atatgattac tactgccttg acagcgacat tcagaaggct
     2161 tcagaggtag acaagacatc aatgctcatg aaagcccttg tgcttacccc tgagcaggct
     2221 gcaaaataca ataatattct cagctactac agctttaagg gtactgacta ctcaacagat
     2281 cagtattatc agaactgcct tgacagacgt gcaaacagtt gttcatcatt cagttatgat
     2341 tcttacggct tcaacgccaa gatcgacctt gacaaggata accttgtgtt cttctcagtt
     2401 ccatatgatg acggctggag cgcaaaggtg aatggtcagg acgtggaaat agaaaaggtg
     2461 gattacggct ttatggctgt gctttgtcct gcgggatcaa acgacatcca gttcacatat
     2521 gaaacaaagg gacttttctg gggcaaggta ataacagccg ttggcttcgg ctcgcttatc
     2581 gtttaccttg gcgcagtgag atttacaggc agaaagaaaa aagaagaagc tcagcctgtt
     2641 gaaaagaacg cataaacctg aaagggagga tataaaatgt atcagaacga aaaaactatc
     2701 agtgaaaaag agatatacaa gggcagggtc gttcacctga cttgtgatga agtggagctt
     2761 gaaaacggct acaagacgct tagagaggtc attcatcacc ccggcggagt ttgcatcgtt
     2821 gctcttgacg aggacgagaa tgtttatttt gtgaagcagt tccgctatcc tttcaagacc
     2881 gcacttatgg agatacctgc aggaaagctt gaatacggtg aagatcattt ggagtgcgga
     2941 aagcgtgagc ttaaagagga gataggtgca acggcagaaa aaatgactta tcttggctgt
     3001 atttatccta cagtggctta tgataccgag ataatccata tgtatatggc agagggactt
     3061 gatttcggtg agcagcacct tgacgagggc gagttccttg atgtaacaaa aatgcctctt
     3121 gaagaagctt acaaaatggt catggaaaac aagctccctg acgcaaagac aactgcggct
     3181 atactgaaaa cttatcttat caaaaaggat atgtagtcca ttatatttta accatgcgtt
     3241 aaaagaaagg gtcgttcgct gttc
//
LOCUS       DAWEMR010000242         3213 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_10092_length_3213_cov_8.187460, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000242 DAWEMR010000000
VERSION     DAWEMR010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10092_length_3213_cov_8.187460"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          934 a          738 c          633 g          908 t
ORIGIN      
        1 ttatataata cacgatatat ctggtcagtt gcttcaagaa gaatgtcaat ataagttata
       61 agcaatatca aatgattaag atcgtgatta tttctgaaat caatggaatt aagctgtttt
      121 actgcttcac gactccatat aatgccagaa tgaacagtat tagctcttga gtgattgtcc
      181 ttatcatgaa tgtagtaact gatgcgacga tccaacagcc tattacagta acattcagca
      241 aacttttcaa tatattctgt tggtaagttc atatcgtact cctaacataa atgaaaattt
      301 acatcagctt atagcagctt actatgctga aatctttaag gaatgtgagc ataccgcaaa
      361 aatcagcgac tacgctgata gagtttttag tggtggtaca ccaactacct caaatgaaac
      421 ctactggagt ggagagttca aatggctttc atcgggtgaa acttctcaaa aatacatcgt
      481 agatactgaa aaaactatta cacaggcagg cgttgataat tcttctaccc gattagccaa
      541 gaaatatgat actgtaatag catctgctgg tcaaggtcac accagaggtc aaacctcgat
      601 gttgcttaca gatacctata tcaatcagtc tatcatagtt gtagagtcgg aaagaatata
      661 tatgccgttt atttattgga atatctgcgg aagatatgaa gaattaagag taatatccaa
      721 ctccaatagt atccgtggta gcctcactac aaaaatgatt tcggcattta cttctcctaa
      781 agctgatata acgcaaataa gtagattttc agaattagct tgggctgcta tacacgaaat
      841 agaaaagaat tgcaatgaga tagcaagact tgccgccatc cgtgacacgc tcctgccgaa
      901 gctgatgaac ggcgagatcg acgtgtcgac agcgcagatt taggcggctt gcaggcgtaa
      961 attttcattt atcgcagtat ttacagaaat cttattacct atgctggata atacgcctgc
     1021 gattttatgc tgtgtattca tattgggcat aggaatagtc atttttagaa aatgcgattt
     1081 gggtaattga ggctgcgcac ttccgctttg tattgataag atttgattat agaaccattt
     1141 gcttcttagc acttgatata agtagtaatt atcaatctta tcaccacacc ttataataag
     1201 cattcctgaa tttatacgga tattatcata aggcacttca tcagagtata ttgcaacatt
     1261 acccacagta ccacgagttg taatcacaat atctccacgc attagttttc cattattcag
     1321 caactcatct ttttttgtgt gataaattga ttatcagcga ataagaaacc attttgtgta
     1381 acattcttag cagaaaggaa aaggcaatga cctgcttcaa gtaattcatc ttgatgagga
     1441 tagttttttc ctctatcacc gtcaattaca tcaatattaa tatcgcataa ttgaacaata
     1501 tcaaatgtca tacccaatcg cccccagctt ctccctgatc tcattttcaa gctcatggga
     1561 cttcttgaac atatccgaca gttccgaggt caggcgcttc attttctcat cgaacggctc
     1621 gccgtcatct tcctgctcct caatgccgac atagcgtccc ggtgtaagga tatagtcctg
     1681 tgcagcgata gcctgaatgt cagccaccgc acagaagccc ttgacttcct ctaatgtgcc
     1741 gtcctgaaat gccttgaatg tatcagcaag tttctgaatg tcctcgtccg tgaaatcacg
     1801 gtgcttgcgg tcaaccatat aacccatttt acgagcatca acgaacagcg ttttgccttt
     1861 ctgcttctta ccctttgtaa tgaaccacag cgttacaggg atagtgacgg agtagaaaag
     1921 ctgtgtcggc atcgaaacga taccctcaac caagtcggct tcaatgatat ttttacgaat
     1981 gtcaccctca ccgctggact gcgttgacag cgcaccattt gcaagcacca gaccaatttt
     2041 gccgtttggt gcaaggtggt gtatcatgtg ctgtatccac gcatagttag cgtttccggc
     2101 aggcggcaca ccgtatttcc agcgaatatc gtctttgagc ttgtcctgcc cccagtttga
     2161 gaggttgaac ggcggatttg ccatgataaa gtcggctttc aaggtcttgt gcaggtcgtt
     2221 gaagaacgtg tcagcgtgat actgtccgaa atccgcatca ataccacgga tagccatatt
     2281 catcttagcc attttccaag tgtcggcatt ggactcctgt ccgtaaacag agatattgcc
     2341 cctgtttccg ctgtgagcct gaatgaattt cgcactctgc acaaacatac cgcccgaacc
     2401 gcagcaagga tcgtaaacac ggcagttctc aaacggacgc aatatctcaa caagggtacg
     2461 caccacgctt gacggagtat agaactcacc gcccttgaca ccctcgtagg cggcaaactg
     2521 agcgatacag tattcataag tgcgaccgag caagtccttg ctgtcctccg tgtcggtcat
     2581 atccatattg gtgaacagat cgacaacatc accgagaaca cgcttgtcca agtcggggct
     2641 tgcgtagttc ttcggaagaa cgtttttcaa tgtcttgttc tcgtcctcga tagctctcat
     2701 agcatcatca atgaccgagc cgatctcagg agtgtgagcc gcagcggaca ccaccgacca
     2761 ccgagccttt tcaggcacga agaaaatatt gtccataagg tatgcgtcag gctcgtcctc
     2821 gaagccgtca ccctcagcca caagctcgtt ataacgcttc tcaaatgcac ttgatatgta
     2881 acgcaggaag ataagtccca cgataacttt tctatattcg gcagccggaa tatgccccca
     2941 caacacgcag gcagcgtccc aaatctgctt ttcaaagcca atattggcgg tattcttttc
     3001 agccatagtt tttacacctc ttaaacctac gcttacagcg aggtattatc tattatatta
     3061 tagcacaatc tgcacaaata gtcaatcatt cgacaccgaa aaatctatat ttttcactgg
     3121 ggattttcgt attaagtaca attacggttg ataaaacgaa aggcgctgta aaaaaaagcg
     3181 caacaaagcc cgtggattcc ctcaatatgg aag
//
LOCUS       DAWEMR010000243         3187 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_10184_length_3187_cov_9.330779, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000243 DAWEMR010000000
VERSION     DAWEMR010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10184_length_3187_cov_9.330779"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          929 a          635 c          571 g         1052 t
ORIGIN      
        1 ttttgcggca aatactgcaa ctagatatcc gccaaagctc atattcgccg aacttagttc
       61 aaaaccgctc agatattgca atggcatatc aagtttaaag ccgtttgtat cgtaaataaa
      121 aagagttata atatccacag tgtaaattat caaaacaaga attattacag caacaaagcc
      181 ggtacaaagc ttgaaaagtg agaatttcct cacgcccgtt ttggttatcc caaagctttt
      241 tatcctgctg ctttgtatct ctgatgaaaa tatgctgaaa aggagcagat aaacaccgaa
      301 tggtatgagt atttcagatg acatatagtt ttcaaagccg tcagcggctc tggtgtcaat
      361 gatatcgtcc gactttttta ggtctgttat cacggcgttg taatcgctga tgagcttgtc
      421 ggaaagcttc aaagaatact catccgtaac gccacgtctt gcatttcggc tgtaacctct
      481 cacacgctcg gatatgagct tcaaagcatt ttgctgggaa tatagctctc cctcaataag
      541 agaaaacata ctgccaagtc ctgcgcctgc ttgtgaattt ttctgagaga aatctattat
      601 cttttcctca agttccacag gcaattcagc ctgctttaca ccgcctgtgt tgtggttatt
      661 ctgatagtcg tttatctcgt tgttaagagc gttttgctca tcagcaagct tttgagcgtc
      721 ttggcttaca gtttccgagc cgcctttaag ataggtcatg tatttttcgc cataagcata
      781 agaaggtctg agtacagata tcaaaaaggc gcaggaaagc aaagccagca ataagcttat
      841 caaagctgac ttcgccatat cttttcttat cataaatctg agaagtttca aatcgtcaca
      901 cctcaaacat caagctctta catttctgac tgtatctctg catatgtatc ataatcccat
      961 tcgccgtttg aaataaactt gtgcagttta aaatctttat ccacaagata agcggctgta
     1021 tcagcgtcgg gatcataggt cgaaagaagg aaataatcct cacagttatt gaatattgtc
     1081 atatagtcga tatactcata gccgtaaaaa tacttttcat tgagcttagc atcatcaatt
     1141 atcttctgcg gtttattatc gttatcaaga taataaagca catactgata cattttgcca
     1201 ttatcatttg gctcacgaac aagcactatc atagtgttat tgaaatactg aaaatatctt
     1261 acaaaagatt tgtctttaaa agtgcaaact gtgtcacttt tttcattatc ctcattatac
     1321 ctatacagct tgttttcgcc attagagtaa tacacatctc cgtcggtcgg ataatacata
     1381 tacggcgtat tttcgcttat ctgagagcca acatcaatgc ctgtatcctt gacgttctga
     1441 gggtctgaaa gtggagctat aaataggcgg actgtatccg tctttgggca aagccttgta
     1501 aaataaagct tgccgtcgct tatgtaatag gtattggctt ttgttatctc gcttttgtca
     1561 gagtttttga gagcgtattt atatagcgaa tagcctgtgc tgaatccgtt ctcgtcggtc
     1621 tcgctctttt tgtatttgcc ctcaacaaaa tagatatctc cgtctttgtc catagacata
     1681 cagcctaata tgctgtcctt tggtgcagaa tacacttccg tgacttcacc gctgttgtaa
     1741 tccactgtga caatgctgta atcttctttt tgcgtagcag aattatacac tgaccttaca
     1801 gcgtatataa cgccgtcata tatggtatag caggacaagt ttgaatactc ttgaagcttg
     1861 acctttatca ttgcaaggct gtcggattcg atatcatatt ctgcaatgcc gtcataggtt
     1921 ttgtaataca caaaattgtt tgcaaatgca gtagaagaag cttcaggaca caaaaggttt
     1981 gtgggagaag cagaaagttc ttgttgaccc ttttcagaag aaaccgcctt atcggatttt
     2041 gtgccaagag aaatgaaaag tgtaactgca actgcggcaa ttataactgc ggctgttgcg
     2101 ataataattt tagttttcat aattcacact caagttctat tattgactat caacggaaag
     2161 cgttgtgctg taattgccat gtaccttatc attataaata tgtacagttt ttactaactt
     2221 tgaagatgta caacctttta acccaaacga atgctcaatt ttacctgtgt cagaatgaga
     2281 tgatccacaa ccagtaccac caacagaggc acttgcataa acataaccgc tgtatttaca
     2341 atttggattg taaacattgt tattgttact tgatgtccta gtccaagctt gaccagctac
     2401 agttgaattt gttcttattc ctgttacaaa acatgaaata taagcatttg agccagaacc
     2461 accaactgct ctgccgtatt ttatggcttc atacgcacta gccccaatag ccatagagct
     2521 catcatcata acagcagcac ccatagctgc gatccttttt acgatctttt tcatattaaa
     2581 cgtcctttct tattataaca tccaaatatt tgaatgtcaa tgttttgttt tttgattttg
     2641 gattatataa attttattac tgcattggaa tcacctgtcc tttctgtaca cagcctatta
     2701 ttttagcagg acgctgtttt gctgtaaata gttatggtct cataatcaga accgttatag
     2761 acctttgcga ttgttttaag acggtatgta ccgctgctta aagactcttt ggaatttgaa
     2821 taaatcgcat accaactgtt gaatgttttt gcccaactac tgtatttatt ccatgaagat
     2881 tcattttttc ttcttaagtg tttgagttat aacgatttta gtagttgtac ctgaaatacc
     2941 acgtacagtg gatttacaag ttgcagtctt gttggagatt gacaatgtac ctgatatttt
     3001 atctgtgtat aaataacaaa cagaaatgtc atcgtcagaa ggcgtttgca gtttggattc
     3061 ggcaaaagct gtaagtgaca ggcatagcat aataacaacc gatataattg tagatatcac
     3121 ttttttcata tatgtaagtt cctcctttca caaaaaatag gtgcaagaga tttattttct
     3181 cctacac
//
LOCUS       DAWEMR010000244         3158 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_10279_length_3158_cov_15.044151,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000244 DAWEMR010000000
VERSION     DAWEMR010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10279_length_3158_cov_15.044151"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1005 a          721 c          770 g          662 t
ORIGIN      
        1 cttattgaaa tgtatcacaa cttcggcaga tatcttctta ttgcaggcag ccgggaaaag
       61 accctgccta caaatctcca aggcatttgg aacaaggata tgtggcctgc ttggggctgt
      121 aaattcacta taaatatcaa taccgaaatg aactattggt gtgcagagaa ctgtaatctc
      181 tcagagcttc atatgcctct tatcgaccat atcgagaagc tccgtccaaa cggcagaaag
      241 accgcaagga atatgtatgg ctgccgtggc tttgtctgtc accataacac cgacatatgg
      301 ggcgacaccg caccgcagga cttgtggata cctggaacac aatggccaat gggtgcggca
      361 tggctctgcc tgcacatatg ggagcattat ctctatgttc aggacagaga gtttctcagc
      421 gagaaatatg acaccctgaa agaagcggca gaatttttcc ttgacttcct tatcgaagac
      481 aaaaagggca gacttgttac ctgcccgtca gtttcccctg agaataccta tctcacagct
      541 tcaggctcaa aaggctctat ctgcataggt ccgtctatgg acagccagat aatctatgag
      601 cttttcactg ccgttgcgga agcttcaaaa atacttgaaa ctgacggagg tttcagaaag
      661 aaagtccttg aagccagaga cagacttcct gcacctgaga taggcaaata cggtcagata
      721 atggaatggg cagaggacta tgacgaggta gagcctggtc acaggcacat ctcacagctt
      781 ttcgcactct atcctgctga catcataaca atgcgaaaga cccctgagct tgccaaagcc
      841 gcaagggcta cccttgaaag acgtctttca cacggcggcg gacacacagg ctggtcaagg
      901 gcttggataa taaaccactg ggcaagactt tttgacggcg aaaaagtgta cgaaaatgtt
      961 atagcacttc tttcaaactc cacaagcgag aatatgttcg atatgcaccc tcctttccag
     1021 atagacggaa acttcggtgg cacagccggt atcacggagg cacttttgca aagcgagaac
     1081 ggcgagataa ttctcctgcc tgcactgccg aaagaatggt cggagggcag cttcaaagga
     1141 ctttgtgcaa gaggcggctt tgttatcgac cttgagtgga aagactcaaa gataacagcc
     1201 tgccacattc actcaaggtg cggaaagaag tgcaggatag tgtgtgacag tgtaaaggta
     1261 cacactgcaa gcagcgaggt acaaaccttg tacacagacg gcgcagcaga gtttgacacg
     1321 gaaacaggta aaacatacac gctcacattc tgagcaaaag acaaaatttg agaggatatt
     1381 gaaatgagac attatcggat aatggcagcg gctgttgcag caatttgcac gctgtcaggc
     1441 tgcggagtgc agataggcag aaacgagagc atgaccgccc ccgaaaaaac atctgttgta
     1501 accacaacaa agaaacagac aaacgatgac agcaaggctg acgagcaggc tttaaaaagc
     1561 tacgaagaaa caaagatagt ggcaaacgcc ataccttatg tgaagttttc aaaaagcgtt
     1621 gaagccgaaa caggctcagt ttcaggcaag gcgaaagtaa aaagtgaccg caagggctac
     1681 aagggcaagg gttatgtgac gaacatttcc actgaggaag attggtcaag agagatcgag
     1741 cttaccgact cacagtatta tgaccttacc ataacagtgg cttccgatgt gccgtgtgtg
     1801 aacggcatag ctgtgaacgg caaaaagctt caggattttt cagccagcgg ctcgggcaag
     1861 tttgaaaagg tgactttcaa gaacatctac atagaaaaag gcaagacggc aatatccgtt
     1921 atccctgtgg acggcggact tgacgttgac tgcctgaccc tcaccgcaag cgaggatata
     1981 tcaaagcttg accttactat atcaaagcct gctctttcaa acaaagactc cgactacaac
     2041 gcaaaagcgc tttatcagta tctttgcgaa agctacggca agcaggtgct tttgggtcag
     2101 catgacacta tcggcacatc tgccgaaaca gacatgatat acaaaaccac aggaaaatat
     2161 cctgccatac gttttggcga ccttatgctt gccactgaaa aggacagcat taccaccgac
     2221 accgaaatga acatcgccat ggattgggct tcaaaggacg gcatagtggg ctatatgtgg
     2281 cactgggctg cccctgacga caagcgtgaa tattatgccg accagacgga ttttgacatc
     2341 aaaaaggcag ttacaaagga aaatatcgca gaactcagcc ttgaggatat caaaaagctt
     2401 caaaaggacg gcaaggtaag caaagaatgt gttgctgtcg ttcaggatat cgacaccgta
     2461 tctgagaaac tctccaccct ccgtgatgag ggaatagcag tgctttggcg accgctccac
     2521 gaggccagca acggcgattt ctggtggggc aatgacaagg aagcctacaa atggctttgg
     2581 aagcttatgt acgagcgtca gacgaaatac cacaagctga acaatctcat atgggtatgg
     2641 tcagcacaga atgccgattg gtatgtgggc gacgaatact gcgacgtgct ttcctgcgat
     2701 gtttatgacg acggcaataa ggacgcacag gtgaacataa tgcttttcct gcaaagcata
     2761 tcaaagaaca agcctattgc aatgagcgaa tgtggaagct tccctgacat acagagcatt
     2821 gcagacgaaa aagccatgtg ggcgttcata ggtcaatggg gcggaaatta tcttatgacc
     2881 gatgacggca agcttgctga agaaaacaac accgccgccg agcttatcaa gatgtataac
     2941 aacaatctca ctctaacaag ggataagctc cccgacttca cccaccttgc aagttccata
     3001 aaggacacgg aagaaaaatc cgctgagagc aaaaagaatg atagctcaaa ggctgacagc
     3061 aagacgaaga aagaaaatac ttctaaagca gaataggaaa aaaggggctg taaaaaaacg
     3121 cgcaataaaa aaagactgca accaccctta aaagggta
//
LOCUS       DAWEMR010000245         3103 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_10455_length_3103_cov_13.841207,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000245 DAWEMR010000000
VERSION     DAWEMR010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10455_length_3103_cov_13.841207"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          856 a          870 c          559 g          818 t
ORIGIN      
        1 agcctttgta ctgctccaag ccctccagct ttctgatagc gacatcgtta cgctccatta
       61 tgcccgaaac tttaactccg tccttttcaa gctcttcaac aagaagcctg aatatcatat
      121 cctttatcat atccatgccg taggaaagca cctgcgaaac aagtatatct ccatacttgt
      181 cgacagtaag ccccggcatg ccgtcagcct caccaaaaat aagcctgcaa gccgaaaaat
      241 cgtcccccat aacagttttt ctgtaatcta tagcatagcg gatacgtctg tggaaaaagt
      301 tttccgaaaa attttcattt gcgttgtcag ataagatcct tatccttatc ttgctgttaa
      361 gactgagaaa accagttcca agataagagc ctttttcaga gaaaacatcg caaagctcgc
      421 cgtttttcaa gtcctcaggc tcgtttctca gttcgtcaca atacacccat gggtgaccgt
      481 ttttcaggga catttcccct tttttcgata tagtgaatgc aggatagatt cttttgcttt
      541 tcattattat acctcacaat caagatttgt cgatttgtca gatataatta taccacagca
      601 tacccatttt tgcaagacga tacacaacaa taaaaatgta gaggacggcg tcccgatagc
      661 catgtcccac agcaataaaa atatgtatcg ccgcacccga aaattttcag atgcggcgtt
      721 atatggggga ttataacatc agcactttgt attacaaagc accaaaatga agaaagctta
      781 tttgtccgca gggtcaataa actcgatagg gtctatccac tgaccgttct ttttcaagcc
      841 aaagtgcaga tgagaaagct ctgccacttc cgcctgagca gtgtcgccca cagcacctat
      901 gacctcccct gcattcacct cgtcgccctc cttgacggac atttcctttg acagattgta
      961 gtaatgccct acaatgccgt ttccatggtc gatagtcatg cagttgcccc acagagggtc
     1021 gtcccatatc tttgtaacag tgcccttgtt catcgccttg acaggctcgc caaggtcagc
     1081 tttgatatca actccatcat gggttttcca gtagccgaga gtttccgact taacaagctc
     1141 gccattagaa aacggctcta atatctcacc gttcacaggc ataacatttg gctgcgtctg
     1201 aatgtccaca tccggcttgg agctttcgtc cttttcaacg tccgttttct tgttatcagc
     1261 ctgctttggc gcagtgctgt caacgctcga aatagtcaga ttgtcggtga tattctccat
     1321 agccttgtta tatgcagcta tacccgcacc gccaagagcc agcagacacg ccgcaagtgt
     1381 aatgtaaaca cccttcgctc ccagagcctt gcctgcgtgt gaatttctga tactgaattt
     1441 tttcatacac acaaaacctt tccccgatat gatttccgca gccactgttg cgcccacggc
     1501 agtcttatgc tatcggcggc ataagtcctc tggcattatt tttgacatat acgggtaaaa
     1561 tattcacagc aaaataaaaa taccgcaccc aagctttcac ccaagtgcgg tattccctat
     1621 ttctaatcac atttatgtag gggacggcgt cctcgacgtc ccacaaagtc caaattgtcc
     1681 ctatccgttt tgtatctttc ttttgtcctc tcagccttac ctatcctgcg gttttttcct
     1741 cacaaactta tacacagccc ctgcaatagc aagcaccgcc gcaactcccg ccagacaaag
     1801 gcagactata acatcatagc ggctgttgtc agcacctttc accgaaacta tctcagacag
     1861 tttcatgccg ttccacgcat tgctcagcac agtcattgcc gctacccata ccgcagatgc
     1921 cacaaagcaa agtgcaccct tgaaaaagcc gttcaccttt agatacctta taataaggaa
     1981 cacagcccac accaatgcca cacaagccac cgttgcaagc atgccatcac gaaggtctac
     2041 cacgtcaccg tatttcagcg taccaaaaac aacgcaggca aaagtcagta ccgaatccgc
     2101 cgccatgcac acaagcccct tgctgtttct cacatacttt tcaagctgtt cacttctcag
     2161 cacaaaaggc aaaaatacga tagcctcccc aaggagcgtt ccaagcaccg ccataacaaa
     2221 ccaatccccg tgagaataaa tgcaccctgc ggcgtaaaga agcacaagcg agaccgtcgc
     2281 agaacctaac gtccataacg ccctgttttt cttcacaagc acaggcacat tgatgaaaga
     2341 aaacgttagc atcagcgacg tgataagcac catgaaccaa cctataccgc catgagccac
     2401 attataaata aagctcggta ttatcgccgc aagatagcct atcccagtta tgatagtgta
     2461 cccccgaaca aagcttttat aacttcgcgc cataaactca gcctctctct gctgattgtc
     2521 ctcgctcgcc gtgcaaagct cgtgctcaga aatgcccagc accctgcaaa ttcccgatat
     2581 catcgttata tcaggatatg atacccctct ctcccacttg ctcactgccg actcagtaac
     2641 ataaagctcc ttagcaaggt ctttttgaga aagccccagt tcttttcttt ttgattgaat
     2701 gaattttcca agagtaattt tatcttccat agcatatcac ctttcaattt gttcggagtg
     2761 cactctctat ggtcaacatt atagcccacg gctttgaaaa agtcaacaaa atcgggctgg
     2821 actgtcagtc aagccgccta tctacgcact ttttcgcatc tttttcatct tatcactgca
     2881 ctatccacac ccacaaaaca gcacaaaaaa tcgccctgct aaaaagcaag gcgttactgg
     2941 agcgggtcag agggatcgca cctccatctc ctttcaggaa gaaaagtatt ctggtaattg
     3001 aaataaaccc gcaaaaaata tgaagtcctt actcatcgta aggacttttt tggagcggat
     3061 tacgaggctc gaactcgcta cctccacctt ggcaaggtgg cgc
//
LOCUS       DAWEMR010000246         3067 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_10581_length_3067_cov_9.925631, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000246 DAWEMR010000000
VERSION     DAWEMR010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10581_length_3067_cov_9.925631"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          874 a          703 c          584 g          906 t
ORIGIN      
        1 cgcagtacat acaactgttg atttgtaggg gcgaacagtg ttcgcccacc ttttaaaaac
       61 tttcaaccaa aagaccgctc atcaaaaagc ggtctttttt catacaaaaa aatacatcgc
      121 cgcaggcgat accataattc ctcactcctc attcctcact agtagtacaa cgtttcgatt
      181 gacacaaaac tccacctgtg atataatttt cttgtaaaac acataacgca aatatggaga
      241 atataaaatg aataacgata caataaaact tatacggaaa acctcaaacg taaattcact
      301 tatggtgata attttctata tgcttgatat cgcacttata agacttggcg acttggcggt
      361 gtaccatatc gcaggaaagt ccgcctacta tgaggatatc gctcagcttg tgagctactg
      421 catacagtat ctcgtggttg tgccaatagt tcttgttgta ttcagacttg taagtggcaa
      481 aaaagagggg cttaaaataa cagcctgttt cagaaaaccc tcaatgcctg caaaatgggt
      541 agcaaagtgg attgtcatat cattcggact tatctatgcc tcaaattata tcaacacatt
      601 tattttctca tatgtgcaga aagcttcggg aacgagcctt aaaggcgttg acttcacttc
      661 aagcggcact gctctgggcg gcgtaacgag cgtgctggca atgtcagtgc ttgcaccgtt
      721 ttttgaggag cttatgttca ggggcacgct tttcagaaat gctgaaaaat gcggacagct
      781 cacaggcatt ataatggtgg ggctgacatt cggactttgg cacggaagct atcagcagat
      841 actctatgcg gctgttatgg gaatgtgtgc ctgctttatg gttgcaaaaa caggatctat
      901 cttcccatct ctgatacttc acttcaccat gaacactatc ggagcaattc agagcgtcgc
      961 acttggttcc atagactttg acaagtttgc cgcagacctt gaaaaaggtc agatcacagg
     1021 cacagcacca atgataataa ttgctgtcgc actgctttca ttcggactta tgatagttgg
     1081 tcttgtgctt tttgtaaaag agcttaggta tcaccgtgac agcttcaggc tcataaactt
     1141 ctgccctgac gtttcacaag gcaaaaagat agtcatctat ctcaccgcac ctgttaccat
     1201 actcgccttt gccgggctta tcttcatcac agtccgcaca gctttaggtc ttgggctatt
     1261 ctaaaatacg cataaaaata acgcagactt tttacagcct gcgttatttt tatcaggagt
     1321 tatttattat gatttaaggc ttgcgatctt ctggttaagt tcctttatct cggaatctac
     1381 agttgcagaa tacttctcac gaacctgcgt ccatgtggac tgtacaccct cttcatcaac
     1441 atttgaagca tcactgtaga gtgcgtcaac aaggcactgg tttccgtcaa actgctttct
     1501 gtcgccgaga gcggaagata taccgtaagc atagtcaaag agcataaggt aatcgtcaga
     1561 aacaacgtcc attttcacat catacatctc gtctgtccaa ttagggttgt tctccatgaa
     1621 cttgtccttg tttgtctgct catacttagg atctgtcttt gaagctctta cgcactcgaa
     1681 ccaacactta acagcttcgc ttcttgttga accttttacc cacatgaaag ctgtcatatc
     1741 agacgttgtt atcttctgct gattatcgtc atactgaggg ataggaacaa cgccccagtt
     1801 ctcgccctcc tttggagttt ggttgcctgt gtaagcccac tcgcccattg catagaacaa
     1861 gcagttctga ttgaagcaat ctcttgcaga gcctacccag ccgtttagga taagtccgtc
     1921 cttcatcata ttataaagga agttctgagc cttttcgata tcagggtcgt taaggttgct
     1981 tgaaaattcg tttgttgcag gatcatagtt tacaagacgc ttgcccgtct gctgaacaac
     2041 gtgcgctctg aagaagccgt ttacgccgta acgctcagta tcagccggtg cgttgccaac
     2101 atatgaggac ataatatcct cccaattctt ccaagtccac tcattgttta cataaagctc
     2161 atatggatcg tcaaggccct ctgcgtcaat gatatcctta tcatagcaga gcatcgcaga
     2221 agcagaatag ccaagaggcg caacatagtg ctttccattg agcatgaact gatctgctgt
     2281 atcctttgtt gcagaccaca aaggctggct gaaatcaact atgctgtcga taggctgata
     2341 catatcctta actacctgtg aaggaaatgc aagccactca tacttgaata tatcaggaat
     2401 atccttgtta gctgtgatag cagcagcaag attatcaaat ctgtcctcat ttgaagtctg
     2461 ctggaatctt atctttccgc ccttgctctc aaaaagctga agctctgtgc tcttctcagg
     2521 actgccctta gtagggttaa ggtcgccctc accgagataa agtatctctt tttcagcacc
     2581 ctcagggata gcctcgatag attccgtgtc agctaccgca acgttctctg ttgcagaaaa
     2641 atcgtcctcc gccttgcttg aagaatctga tgagcttccg caacccgcta ctcctgttgc
     2701 cattgacaaa gccattacag ccgctatgta ctttttgtaa ttcataaaat attcctccat
     2761 aattaaatat tttagcacca aagctgtccg tctgtagttg atgagttcat atattaaatc
     2821 ttgctataat tatacaacac atataaagct attgtcaatc accattatag cacaattatc
     2881 agcaatttaa ttgtacactt tgcacaatta aatccatatg tattgttaaa acgcaatatt
     2941 acaatcaata tagtttaaaa tatacgcata aagttcaaat attcaatatg cccgttttga
     3001 atgaatataa aacagataca agagcttttt tgtagaaaac attaaccaaa acagcaaaat
     3061 atgtaca
//
LOCUS       DAWEMR010000247         3059 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_10614_length_3059_cov_7.327230, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000247 DAWEMR010000000
VERSION     DAWEMR010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10614_length_3059_cov_7.327230"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          745 a          662 c          668 g          984 t
ORIGIN      
        1 gatgatatct ttatcggaag tttcatagac ggtctttatg ccgttttcaa gatattttat
       61 acaatctgta agcgctccgt cactgtttat atccgggtta tcgtggagcg aacgtctgat
      121 aagataacgt gtatttgaaa gctttgcggc gacagtattc ctgataaggt cgagctgagg
      181 ggtagaaaaa agctcaaact gctcttttcg cagatagacg ttgccctttg ttttgccctg
      241 aactcttgcg agaaatttac cttgaggaga aataaaatta acggctatgc catattctgc
      301 acacttgccc ataagcgcag gtgaacagcc agaatagctg aaaatataga tatcctcgat
      361 attagtaaaa ggcacacgga acttctcgct gtcctctgcc ctgcacacta tgttttcgcc
      421 gtcaagtgtg aggtaggcag tttcttcgag aatataaagt gaattgagca gtttacgcat
      481 tgttttcgcc tccgttcatg gagaaaatcg tatttttcac gctatatttc ttcacttttg
      541 gaaagcacat atccgacagt gaacagcctg aacatttctg accttttaca cgctttggaa
      601 taatatccgc tgcaagtatc tcacgcattt ttccaagata gtcgcaaagc attttatcat
      661 actctgcgcc ctgagtatca aagggcaatt ctgatcgtct gcgcacatct gcatagtaca
      721 gaaaacatcg gctattgcaa tgaaatacgt tatccacgca aagcttctgc gcatagactt
      781 gtatagcgtc ggtctgattg aactcccctg ctttgggtct ggtgggttta tactccacaa
      841 tttccacacg aaatgtgccg tccagggtat ctatatagtc gccgctttta tcacggacaa
      901 actcgataca atctacaact ccgaaaatat tgtacttagg gtcatcgtta tacactgaca
      961 cgtcacttcg gacgatctta tgcttatttt caaagttgcg ctcgccgctg tggacgttct
     1021 catggaggag gtttgctttg acgacaaagg cgttttcagc ccagtcgtca ttaacttcta
     1081 aaagagcaaa cctgcgtgga caatacatat aatgctgtat gtcacggata ttgatagtta
     1141 catcttcgct catcagagct tttcaataag ggtaacgccc tcaggcatat ccttatctat
     1201 ggagatatca tagtctgagt aatgtcttgg tgttgtcacg ccgtctttga gcttaactga
     1261 caccttgtca aagaggatat gtgcaggggc attgcccagg gcggagtcat gcttgaacac
     1321 gataagctta cgcatacaaa gctttcctct tgcggcgctt ctgtcatgct cgaacatatt
     1381 gatgatagat gtccaaagaa gctcaaggtc gtcctcacta aagcctgtta tcttctgagc
     1441 gagcattgcg gaaacatagc cctcgccacg atagagagcg tatgggatta tgctcttttt
     1501 gcccatttcg gtctcaccgc tctgctgtct gtcttctgtt gttcttgcct gtcttgtgat
     1561 tgtgatatcc tgagcaaaaa cagggtcgat acttcttgca aagttgagct gtacaggacc
     1621 tcttacaata ccgcacgggt catcgcccgt actcattact gcaccgaaag ttcttacgtc
     1681 aaagtagttc tgacagatat agtcacgagc ctgcttaacg tcatcaggat tcttgccttt
     1741 ctgctttgtg gcaagaccaa gggtgttata ggcttcggtg aactttgtat tgagggcttt
     1801 gtctgccttg ataaggatat tgtagcctgc ttcgccctct ttggtaagct ctacaaagtt
     1861 tcttatcttt ctcttaaggc acacgtctgt aacgataccg aggcttgttt cagggtctgt
     1921 tctaggcata ttgcctgcgt cagggtcgcc gtttgggttg ccgttctcaa cgtcaaaaag
     1981 cattacaaat tcatatctgt tcttgattgc gttttccata atatttcgtc ctttctttat
     2041 tcttctgatt ttttggtgta aagcgcttta ttctgctgtg catatcctac aataaatctg
     2101 ccctgatcgt ctagggagag tgtagacggg aatttgccgt caagtccgtc aagtatctct
     2161 ccgcagagtt tgttatagaa tattgggctt gaaagcttct tgatatggtt ttgtgaaagc
     2221 ttatacagct ttggaaaaac tgttgacggt tttgagcacg cagaagcaaa gtatgagtcc
     2281 ttaattgttc tgttaagtcc tccgcctgaa gcgtcctgct gaatcttttc aagcactgcg
     2341 aaaagtcttc cgcagagata agcctgattt tggttttcaa ggtctaatga cattttgatc
     2401 tcctccttat tttgtttacg gtttatgcag ccttttatca agcctgccct tgtatcattg
     2461 agttttataa aatggttgtt ctcctcgtca ctgtcggtct tgacccttgt tattactgtg
     2521 gaaagcagtt cgtcagggta tcttgtgcct ttgagtattg cttcaaaaag tgcggcggtg
     2581 agaggtgacg gaactttttc tttggacgat tttggtgaga taagctcacg cttgatacgt
     2641 tcaatgctta cttgatgctg accctcagta aatgaaatat cctgctgatg tttgagtaca
     2701 ttgtcaaata tcctgccgaa cttatcgtgg aagatgaact tctgggatat tctggagctg
     2761 ttgggtgtga agcctgcaac gtaaaattct acgttagggt cataaggcat atcggctgtc
     2821 ctgccctgtt taagctgggt catgacagtg tttatgtttc ggtcggcttc ggtcacaacg
     2881 gttttattta attcgtcctc aaatccgctg aacataccgg caaacatatt gcattcttct
     2941 gcatcgttct tcttcattgc gaaataaact attgtcatat cgtcaaaagt cttgtaatgg
     3001 cttctgtcag caagcagaga attaagcgca gaggtataca ttttcatggc tttctcaga
//
LOCUS       DAWEMR010000248         3054 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_10638_length_3054_cov_8.916972, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000248 DAWEMR010000000
VERSION     DAWEMR010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10638_length_3054_cov_8.916972"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          946 a          608 c          570 g          930 t
ORIGIN      
        1 gtctgcattt tatgttattc aaccacaaaa tatgaccatc gaattgtgga aaatgttcgt
       61 acaacgaatt gaaaaaataa tgtcaactat gattttgtca atttacactt taaagaaaga
      121 agtagatgaa ctttcttcca aactcaagaa gtatgatgca gggaaaatag atgtggctcg
      181 attattagga tttgaagaaa ataatattgt gatcacggtg gatatcatca atcagttata
      241 ttgcaataaa gataaatacg attttacaaa aacatttaat caaaatttgc ctgtaatttt
      301 aaatctggat agtgatatta tagcgagaac gcaggaagaa aaaatggagt atataaagtc
      361 tttagtagcg atggataaag aaaagcaatt atctgagtat atatggaatg gaatctatgc
      421 gttcaatgag atatatgaaa atgaaatgaa tttaacaaaa taaaatatag tttacatcgc
      481 cgagaggttt tcttaacgta agcgtaaaat aaatccacga ctttcaaaca actgaacttt
      541 ggtgctataa atgagcatcc caacaccgtt ttcgtgaaaa tgcacaaaag tggttaagca
      601 atattctcca aaagggcaga cttatcccca ccgttttgag catagtcaaa aatgaaagag
      661 atctgctctc ttcgaatcgt ttgggacagc aaatgatatc cttccgagaa attgtaccgt
      721 tttgattcga agcaagctcc gacagtgtga aagcctttgc atattaactg tacagctttt
      781 ctgatttgtc ccagcttcaa tatgctatgt gcagtatcac attattttag catttttagc
      841 aattcccgtt tcatgaggca caagtcaaac acattaatct tgttatcttc gcataaatct
      901 cctgctttcc aatccgcaag cattgcttcg ggaactgcaa gcagccactt ttgcattgtc
      961 acaacatccg caatattgaa tgcaccatca gcgtttacat cacccacaat atgagaattt
     1021 gtttcagcaa atgttaaaag ataagtaagc ggagaattcc agtaaatggt gatttcattt
     1081 gttgtataac tttcctgatt gtcaacataa cattttcctg tcggtttatc cttgcaataa
     1141 actttagtgg catcgtcctc taaatatgga tgcacaccac ccacaagcat acctttcata
     1201 gccgtattct gtgcaatgga tggtctgtga tgcggatgtt ttggcgaaac tgttccgtaa
     1261 cctgtatagt agcagatacc cagcggattt tcaccaaaca gatagtcaag atctgatttt
     1321 gccgcattga gatatttgca gtcgccggta agaagatacg catttccaag aattattccc
     1381 tgattgcaag ctgtcatatt actgccccaa ccgcctttgt agtaattcgt aacagaaagt
     1441 ctgtaagcgc ttttttcaga tgcactgaca agtaagttgg cattgcttat aagagaattt
     1501 tttgcttttt ggtagacttc tgaatcagtg tcaatatctt tcattgtaac gattgcaatg
     1561 tttccgtaat caccgaaatt tgcagagtcc ataccctttg caacactcat tttttccagt
     1621 gccgaaaagt atttttcgtt gccagtagcc ctccacatct gagcgaccgc ccagtaacgc
     1681 tcgtccttat cagaattgtc gccataagat cctgttacaa tatcggacgg atttgtaaaa
     1741 atgaaattcg gattttcctc taaaaacata taggcatttt cggcagaaac aaggcatttt
     1801 tcggcaaaat tgctgtcgat atccttgtaa aattcataag aaagtgccat tgtaccgcag
     1861 aaagcagctg ttgcagttgt tgaaatcgga gttacaaata attcctccgt ttcgttttcg
     1921 ggcatgatgt aaccgcagaa attcgcacaa ctcactttat gatgtacgcc gccattgctg
     1981 tcctgcattt taagaagcca ttcaatttca taacgtgcct catcgagaat gtcggggata
     2041 ttatttttac tttcgggaat acctgtatta tctccgaaaa gttcaggagc attgtcataa
     2101 gcataaagca agtctgcaac agtttttgca cctgttacaa catacctgcc gtaatcacct
     2161 gcatcgtgcc aaccgccgga aacgtcaatt ttttcagcag ttccgtatac tgtcgcaaga
     2221 gaggtatgac aagccttgtg actgaaaatg tcgtcattaa cagaagttcc gcagcactgt
     2281 aaataaagca ttttaatgga atcctccaaa gcagaattat atacggaatc ggagatttca
     2341 aaagtgtagg aattatcaag attcccgctt gagacgtaat aatttccgga ttctgtaacg
     2401 cacgagaaat cgccgactct cacttcctgt ttcgaaaagc cgtcgtaagt tttggcagac
     2461 aattcgcctg taaatacgat ttttccggtg tcggcattca caacagaaaa agtatcgctc
     2521 tccgcaccga caaatacagc ctttttattg gcatcaggtt tatagcctat ctgatttgta
     2581 acaatcggcg gttcataagt ttccattccg gatttatcaa cattactgtc gtcaatcagt
     2641 ttaacttgca cattatcaat ataaattgta tgttcaggca attcagaatc aaaatagccg
     2701 cagttaaaac aaagttttgt cataatatca gtgtccttgt ccattgtaaa ctcataatca
     2761 acggtctgag cttcagtggt gacactaagc tgtttgtagg tgtaagcctg atatgttcca
     2821 ccgttttgct gtatcataaa gtcaacagga cgattggttg ttgccgatat atcaaagctt
     2881 acacgataaa ctccattttg gtacaacggt atggagtcat cagcaaatgc ctgcacagaa
     2941 tatgtaagct ctcctgccga ttttacgttg acagcaagac gtttattatc aagtgaaagc
     3001 gaacatttgc cgccatttgt gaaatatgtt ccccaaccgt aagaagtgat gtcg
//
LOCUS       DAWEMR010000249         3041 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_10681_length_3041_cov_10.087408,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000249 DAWEMR010000000
VERSION     DAWEMR010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10681_length_3041_cov_10.087408"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1872..1881
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2408..2520
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          849 a          528 c          591 g          950 t
ORIGIN      
        1 tttgcctctg atatttgaaa aaatggctgt gatatattag atttcatatt ttatcctcca
       61 aatactgatt tgatgctacg atcaactatc gtattcagtt ttttctttgt tttgaaatat
      121 tataccacgg cataattgaa ctgtcaacca caccaaaccg gcaaaagctg cttgatttat
      181 ctcttgattt aatttgcaat gtgtggtata atagctataa acctgcgtat aatacgatca
      241 taagatatat gtaaacatgc gtattattga aattaagaat atattttaac ctgcgtatta
      301 ttgaagtgag gtgttgttat gatactaaag cgtaaaatgt ataataaact actgacgctg
      361 aaaaaagaac tgaatggtaa aaaagccttt cttatcgagg gtgcaagacg tatcggcaag
      421 tctactatct gcgaggagtt tggcaagaac gagtacaaga gctatatcct gatcgacttt
      481 gcaaaatgtc ccgatgaggt caagggctat tttcaaaagc atatgaatga cctcgacacg
      541 ttttttatgc tcctgtccac atattatggt gtgaaattgt ttgaacgtga gtcgcttata
      601 atttttgacg aggtgcaaat gtacccaaag gcaagagaat gtattaaata tctcgttgcc
      661 gacggcagat atgactatat cgaaacaggc tcgctgatct ccatcaagga gaatgtaaaa
      721 aacatcgtca ttccgtcgga ggaacgtcat ctgagtatgt atccgctgga ttttgaggag
      781 ttttgcgatg ctttgggtga ggggcagatc attggctata tcagaaaatg ctttgcagac
      841 agagaacccc ttgaaaacga gctgcaccat aaggcaatgc tgctttttaa gcaatatatg
      901 cttgtgggcg gtatgccgca gagtgttatt gcgtttcttg aaagcaggaa ggattttgat
      961 aaagctgacg ttgaaaagcg tgatatactc gcactctatc gcagcgatat tatgaagatc
     1021 gatgcaaggt atcgcagcaa ggtgcttacc atatttgatc agataccggg actgctttct
     1081 cagcacgaaa agcgtgtggt gttcagtaat gttgtcgcac aaagtacggc ggatcagtat
     1141 gaagaaacat ttttttggct gtcagattct atgatatcca atgagtgttt tctatgcaac
     1201 gatccgaatg ttgggctgtc gatcaacgaa aacagaagtt atgtaaagtg ttatctcggt
     1261 gacactgggc tgctcgtaag ccacgcattc gatgagaacg aactgcttga agatgaggtc
     1321 tacaagcaga tactcggtga caagctgaac atgaatgagg gtatgcttta cgagaacacc
     1381 attgctcaga tgctgacggc aaacggtcac aaactgtatt tctatacaca ttacaacact
     1441 gaaaagcacc gaaacgatat tgaaatagat tttcttattt ccaacaacag taagctgaaa
     1501 tacaaaatgt atcctattga agttaagtcc ggcaagcgat attccataga gtccttgaaa
     1561 cgctttaggg agaagtataa ggcaaggatc ggagagtgct atgtcattca tccacgtagt
     1621 ctttgtttca aagaggatat agtttgtatt ccgccgtata tgacaatatg tctgtaaaaa
     1681 accgccgttc gggaactagc ctgaacggcg gttttgcttt tgtgtcactt tatgtacagc
     1741 ctatccgcca atctgaggag tgcaagcagg tagtcaagtt cgcttttcaa cgttgtcagt
     1801 aacgggtcgc cctcacggat acgcatggtg cgccttattt ctttaggtat gacaactctg
     1861 ccgagatcgt cnnnnnnnnn natcctgcgg acaattcctg ttgctttcat atttatgacc
     1921 tccgttgttt tttcaaaatt gcagtaatat tatttgcaga ggtggggaat ttatgcagga
     1981 aaaaagcgag tgataatcgt atggatttta gcttttattg ttcattatca cattgattgg
     2041 gaaatgtagt atataactaa tacgattatc attattatta cgattgcagc ggctataatg
     2101 gcatttactt cattggttgt ccttggaagt tcttcaagat cagcttctaa ataattagga
     2161 tctaactgtt tcaatttttc gtcaactcta tttattgaat caagccattg ccttttattt
     2221 ttatagttat ggcggtagga cgtttttcga catacagaat atatatatgc atattgttgg
     2281 cgtatgattt tccaaagata tgttgtctta tatttgtccg gtatagtatg tatgatatgt
     2341 tctattgatt cgatcattcc caatgatagt tccacgaaca tatcataagt gtattcacta
     2401 tcattagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 aattgtcatc aattacagat atcgaatttt ttaacacttt aattttctgt tctcttttaa
     2581 agcggtcaga atctacaagt gccattcttg cttttccata gctggagtaa aaaatagcct
     2641 caatatttgt tggctcatac tgctctacca tgttatagta tttttcacat tcttcccaat
     2701 cttctttagc ttttgcacgg cgtgcgtttt cgagggaacg ctctacaaaa gaggtgttat
     2761 cgactgtgac tttacttcct gacacatcga ccttgccatt aacttcgaca ataagacgtt
     2821 tagcttcttc aggtgaatat ttagcaccgc aatactgaca gataaagcag ccgtcctctt
     2881 tgactaaatc attgctaccg caaacttcgc atacgatttg tttcatgctt taacatcttc
     2941 tttctgtgat ttgtattttc gtgattaaaa aaattattat atttctggtc cgctataatg
     3001 tggtaaacca gctaaatcca tgttttcgtt ataataatca a
//
LOCUS       DAWEMR010000250         3040 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_10685_length_3040_cov_10.880402,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000250 DAWEMR010000000
VERSION     DAWEMR010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10685_length_3040_cov_10.880402"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1039 a          566 c          666 g          769 t
ORIGIN      
        1 attcggcaga ggacatctcc gtgctgtgcg atactgtgca tgaaatggat aagggagtga
       61 taagtgagat cacgttttga catagggggt atctcacagt gcgacggcga aaacgcacat
      121 cttacccccg tgtcaaaatc aaatcttctg tgcaaaacaa tgaaaatgga ccatgactat
      181 tgacaaacgt attaaaatgc gataccataa gaatggagaa tgataaaaac tcgtaaagat
      241 ttaaacgtct ttgcgagctt tttgttttgt atatattttc gctgtgagca gaaagggtga
      301 aacattaatt gaataaggag gcttgataaa ttggaacaat tcaattcaaa gtcctgcgaa
      361 gaaattatga caaccgtgta caagccaatc gagttcgttg tagacggctt gcttgcacaa
      421 ggtctgtata tcttagcagg cgcaccgaaa gtgggaaagt cttggctggc acttgagatg
      481 tgcttgtcga tagcgaaagg agaaaaagtt ttaggtctaa aaacttcaca aggaactgcg
      541 ctctatctgt gtttgaagac agctttcagc gaatccaaaa caggctttat gaattgacgg
      601 acgagcctgt tgaaaatctt cactttgtaa ttttgtcaga cacaatcgga aaaggtctcg
      661 aagagcagat agagcaattc aaaagtgagc atagcgattt aaaagttatg ttcattgata
      721 ctctgcaaat gattcgtgag tcaaacgata acggttatgg ttcggactac aaagagctat
      781 ctgttctgaa aagtctagct gacaaacttg aaatcgcaat aatagttgta catcacacta
      841 gaaaatgcaa agacagcgac ccattcaata tgatctcagg ttcgacagga ctgagcggct
      901 gtgtagacgg aagtatggtt ctgatcgaaa caaaacgtgg cagccgtaat gcaaaacttt
      961 attgtgtagg acgtgatatt gaaaacgcag aaattaattt gcagttcgat agtgatttga
     1021 aaaagtgggt cgttaccgat gagccgaccg ctccaaaaag taaggccaac atttttcttg
     1081 cggcattgta tgtttacatc aaaaagaaaa ttgatttttc aggtacggcg tcagaactta
     1141 tagagaaact gaaaagtgtt tctgatgaaa cattttatcc caacagagta acaagagatc
     1201 tggttcgcaa cggttacacg ctgagaaaat acggcattga ttttgagtac aaacggacac
     1261 gaaactgcag gctgataatt cttcattacg accgtgagcg tgacagcagt gacagtaaaa
     1321 acactagcga agttactgtc aacaggctga ccttgaaagt atgaaaagtc ctgcaaaacc
     1381 ttgctttaca agatgtgacg gtagaaatca aagcaagttt tactgtcaac atttcaaagc
     1441 tactgtaaaa gccaaatcag cgaacgtgtg gcgatctgtg ggctaaaaaa atcagtcggg
     1501 taagtttagc agaaactcac caaaaccaca cacaagcgat ttgtgagccg ctgtgagcga
     1561 tattttaaat aaggttagct tgtgcaagtc gaactcaacg atttgagcaa ttatgtactt
     1621 ttcacaattc agcagctaga gctataacca ccaaagctaa atatttcatt gcaagttaaa
     1681 gtttcggggt cacagccccc gaaacactca cagcggacga gcaaagctcg ccgcaaaagt
     1741 gccgtaaata cgcagtttta aaaaagtctt tgtgcatatt ttttatgggg tcaagatacg
     1801 gctttcaaag aaaattaggg gtcgcaagtt acagaaatcg cactcaaatg gcgtgaatac
     1861 gaactttgca ataggagtca cagggtggtc aatcaaaagc acggacaata aaaccatata
     1921 gaaacgaggc gataaacaaa atgtgcaaac tgattaacac tatacaaaaa aggcaaaaat
     1981 cgtaataagg agtgttgctt ttgcagataa tcaagaacga gaaatcaggc atcgctcaga
     2041 tctggctgag caatgcagaa caacaaaatg agagagttat gaacttggtt gagtgcaaga
     2101 taaaggagct tagcggcgaa aaatttaaag ttgccgtgtt ccgctcgggc tctaaagatc
     2161 tgtatgaatg tactgaaaac cttctccacc ataatcttac tctctgacag cgagtgcacc
     2221 tatggtgcac tttacataca ctccaccatt atttttatat cctttgccta ttgccttttg
     2281 ggcaaagatg tgatacaata ttatttgtaa taaaacatat aaactaacaa cattgtatgt
     2341 atccgcaagg gtaagatcca taatcaaaag tgacaacatt gtaccatata tcaaagaaag
     2401 ggtgaaagtt atgagcacaa aatacagcat agcaggctac tgccgaattt ctgttgacga
     2461 ggaaatggac agggacaaca cctctatcga gaaccaaaaa gctatcataa ctgactttgt
     2521 aaagcgaaaa ttccccgaca gcacgcttga attttacgaa gatcgtgacc gttcgggata
     2581 tacttttgaa cagcgtgagg gctatcagga actgcgaaag aaaatgctgc gaatggaata
     2641 tgatatcctt atcgtcaaag acttctcaag attttcacgc cgcaacagca aaggtcttgt
     2701 agagcttgaa gatctccgtg acgccggtat gaggatcatc tcaataggtg acaacattga
     2761 ttatcctacc tatgacgatt ggacagctat ccaatttaga tttctcatta acgaaatgcc
     2821 cgttaccgac acctcaaaaa aagtcaagtc agtaataaaa cgccgtcagg aagaaggcaa
     2881 atggatatgt gccgtgcctt atggttacgt tatgaccaat agcaaaacaa tgaaatttga
     2941 tgttgacgag cctgccgcac aggtggtgcg tgatattttt gaaaagtaca atgagggctg
     3001 gggctataaa aaaattgcaa attaccttac agaaaagcat
//
LOCUS       DAWEMR010000251         2968 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_10963_length_2968_cov_9.927909, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000251 DAWEMR010000000
VERSION     DAWEMR010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10963_length_2968_cov_9.927909"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          891 a          597 c          608 g          872 t
ORIGIN      
        1 ttttatcggt tcggagtata ccaaatggta tattacaact atttcagcgt agcattctat
       61 gcagcgatgt atttcctgct tcattttaag aagataggca aaatgtcatt tacttacctt
      121 gtgcttggcg agataatagt acacgcctgc atgggtgctt attacatagg ctggtcagca
      181 ggatttacgc agataatgct gtgtataata cctattccat ttttcatttc acaaaacagg
      241 aaagcgatac catatatact ttcgtccttt gatgttgtag tgttcatagt catgcgtatc
      301 atagtcacaa acagggtcgc accttacagc tttgatacca acagagagaa tatcttatac
      361 atttacaata ctttatgcag ctttattata attatctatg tatcttccat atacattttc
      421 acaaatgagc ataacaagcg tgaagcaaaa gcacagaacg aaaagcttca aaagctcgct
      481 actatcgacc cactcaccca gcttttcaac cgccgtgcca tgatggattt tatcaaaaag
      541 atagaaagca acagccgcag gacaaacagc gtatattcta tgtgtttggg cgatattgat
      601 gatttcaagc acgttaatga tacatatggt cacgaagtcg gtgacaaggt acttagagcg
      661 gtatcagatg ttatagctgg aaacgtgccc agcgagggct atgtgtgccg ttggggcggt
      721 gaggaaatac tattcgttgt tccaaataca gacacagaaa gctgcgagaa gatagcaaag
      781 agcatttgcc agaaaataca tgagtgtaca tttaaggaaa attctcgaag tttcaatata
      841 tccatgactt ttggagttta ctcagtgact ccaaacgaaa actatgacga gggtatctca
      901 aaagtggata agctgctata caagggcaaa aatcagggca aaaactgcgt tgtatctaaa
      961 taaaatcaag gcagccgtgt agaaaaaaac tccacggctg ccattttcat gcctattttg
     1021 cagagagata tcctctcatg tctacagaaa ggcgttcctc aagacatata agctccttgg
     1081 caatgttctt ggacttctcg tctgccgcct tataattgtt gagatatcca gacagggact
     1141 tcacgcccat attacagccg tctgttataa aatctgccac agcgtcgtca ctgctctctg
     1201 ccgcaagctt gaagttggtt ttaagccatg ccatgccctt tgccacagca ggcggttctt
     1261 ttccgctgtc atgatatgaa tttaaaagtc cctgaagctt ctcttgtatc tccacgtgcc
     1321 ctgcaagatt tttctctaac gttcgcttta gctctccgtt tgaagtgtat ttcagcgaat
     1381 cctcaataga ctgcacgccc atttttattc ctgcatcgca ctccctgaga agctttattg
     1441 tatccttttc tatcatctaa gttcgctcct ttcataattg caaatagttt tccacaaaaa
     1501 tttctcttta tgcctgcata aattcagcaa aactattgaa aaatctttaa atttgtgata
     1561 taatttcttc atggcttatg cctgaagtat ataagggggt attttaaatg ggagaacaca
     1621 aacattacaa agagtcgggc aaattcagcc cagttggagc agtgctcatg gtgcttgcaa
     1681 cggcagtatg cggcggactg cttttctggc tctatctgct ttttgagggc tggtgcacta
     1741 tcatctatct caatgttatc gctgcgatag cggtgtcatg cgcacttggc tttgtgggag
     1801 gaaagatagt cagagcattc aaaatgcgaa acacagttgt ggcagttgcc tgtgcagtgg
     1861 tgggcttttt attagcctca tattttaaat gggcgctgta tgattacaac gatctgaaaa
     1921 aatatggcta tgagcttatg gaagaataca cagcctatga ttattatgac gagctgaata
     1981 tgctttttga tgaaaacaca gacggtttcg gtgattatta tgacttttat caccagacca
     2041 aggcatatga tcttttcagc aacgattcaa caggcgattt cacagacaag gacattgcca
     2101 ccatgcagaa aaagtctgta tggcagtttt tcgagcttga caagcttctc ggcaaggata
     2161 aaaaatccgc acagaaaagc cttgaaaagt catacaccat gaacgcttac gactacactt
     2221 atgattacag agattatgat aaaacaggac tttttgacat aatcgcacac ccttccgacc
     2281 tttggaatga tataaagtct atcaacaagg agggtcgctg gtcttacaag tcatcacatt
     2341 catcaagaga taacggctcg cttgtaaacg gcacgatctt gtggatagta tggggcagtg
     2401 aatttatcct tctgctggct ttccttgtgg gcggtgtgag aatcaagtca aaagagcctt
     2461 tcatagagtc tgaggacgat tgggcagaat acaaaaacct caagggtgca tacaaattca
     2521 tggtgcctga ggacataaag tcatttaagg tttcaatgga gcaaagccct tacacgcttt
     2581 ttgattatca gacggctgtt actcaattcc tgcctacata tatggtgata gattgctatg
     2641 tgagcaagtc aggcaatgag aattatctca cagtttatca gcagacgatc aaccctaaga
     2701 acaaaaatcc tgacacaaag gtgcttgtga aaaacctctt tgttgacggt gatttcatga
     2761 acaggctcta ttcccttgca aattttgctc cgcaaatgca ggacgttcag cagactccgc
     2821 agtaaagata acaaaataaa gacatacaaa acaggatgac ttagatttca tcaaaagggg
     2881 atatgattat tataatgaaa tatgttaaat atgtattgat gagtttcata tatgctcttt
     2941 tgtttaattt aactgcatat ttaggagc
//
LOCUS       DAWEMR010000252         2967 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_10970_length_2967_cov_6.293269, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000252 DAWEMR010000000
VERSION     DAWEMR010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10970_length_2967_cov_6.293269"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    588..597
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1821..1830
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          852 a          596 c          441 g         1058 t
ORIGIN      
        1 ctttctgtgc gaacaaatga tgttctacac ttgttctaag ttggtctata gtgtaaaaac
       61 tgtttaatga cataaaaatc gtcctttcct gatataatgt ttacatataa ttgtactaaa
      121 aattgcctat aattatatgt attataccac aaaggacgat taaatgtcaa gtgttcacca
      181 attaatgatg cagggcaagc gaaacataaa tcgcttgttc aatctgcttc ataacattag
      241 gtgtcaaatg cccaagtgtt ttaataacac tagatttatt aatagtcaat agctgttcac
      301 acaaaacggt gctagttttc agtaaaccgc tttcaacacc gattttaaca tgggttggca
      361 catatttttt tgtagcactt gtaatcggta caactattat gcaaggggag tgtgcgttgc
      421 ccatgttatt ctgtacaaca atagctggtc tcctacctgt ctgaactgac tcgcctacat
      481 ttggcagatc aaccaaaatt atatctcctc tagtaactat atttttatta actcttcttt
      541 ctcttgtttc tgtggttatt actggtgtta tggtgttaat catacgannn nnnnnnncta
      601 ttaaacgttt tgttgtctct atttgtcttt ttttgtcata ttttctatat tataatctgc
      661 actcgaataa tagtcaacgt ttatttgatt acggatatgt taattatcta tgaaacggga
      721 cgttttcaaa actgaaatta ccgatattaa aatttagatt tccgacttct gacttactca
      781 aaattctttt tacttcagaa cttattttga atacttgtgc cttgttattt ttactctcgt
      841 aattatcata tcctataact tctattggta ctttactgat aagatggcta ttttgcagac
      901 tccataaacc tgcaaacgca agctcgtgta cataatcgta catgacatat ggtgtacatg
      961 aataatcata ttcatcgttc tccgtgtcgc caaactgtaa atctatatat aaatctttta
     1021 gaccgtcaag ctgttcctct gaaagattac caagtgtata acaatcaatt ggcagtattg
     1081 cttcatgctc atttgtttta actctggcaa aatcaatgta atcaacttta agaaaattca
     1141 ttaaattata acaatccaaa gattgaggag caggcggcaa gggaagtgag ggtacaacgt
     1201 gtgttccatc attttctcca actatggtta gtacaatatc tttacaattt atcataacgg
     1261 tactgttgtt atcttcaacc gtcatttcgg acaattcagt gagatagtct acatcatcac
     1321 ctaagcccaa tgacattatg taatcggcta ataacaaatc atgtacccta tctagttcta
     1381 agacaagcca ctcaggatca tcaaaatacg gcactaattt gtcacttatg ctttttagtg
     1441 acttgtatac aacaggctca tgcgacaatt tcagagccgt tccataaatg tggtctgtat
     1501 ttatgttgtt agtgatgata aatttgttcc ataaattctc acgagcaaat gtcataagct
     1561 cttgtaatgt catttttttc attttataca ctccttttat tcaatctcaa aacgaacatc
     1621 tgttctataa tgtttatact atactacaaa acaaatgctt tgtcaaggga tatttgtcct
     1681 ttattttata cagcaataat tgccatacta attaccacta tcacaattct atcaccattc
     1741 aatgtctaaa tcaatgataa attattccca aaaataaata cacgatttaa cagcgacaat
     1801 agtttcttcg aaagttccat nnnnnnnnnn cgatagaacc ttctttacgg gttgatggga
     1861 atgaaaaaga ctctcccttc tcatttaccc atatctcatg cgacccctta cctctgcgtg
     1921 agtatgaaaa cccacgctcg gcaagcagcc ttttaaattt gtttatgctc attttgttta
     1981 ttgttccttt cttttctaat ttttgcaaga tatgaaaaca aaacttgcat tttatttact
     2041 ttagcctgtt acatcacact ttctcaacat tctaataatt ccactctgac cctttggcgt
     2101 taccataggt gttagtccta ttctgacttc gccattctgt atgtatgagc tttcttttag
     2161 ctgaaaccat ggctgagtgt ctatgtatct ctgataaggc atattcttat gaccgtccct
     2221 acagcctaac actttcttct ccctcagaaa gttaaacaac cttgttctgc ctatctttat
     2281 tccattctta gttgccagct tcgccatatc gttcattgat atacaatctt cagaagtttg
     2341 tatatgactt gcaaagtcca caagaggttt atcctgctct atcttattat taagctgtct
     2401 gatcgttgat agattgagcc tgaacagttc tctcgtctga gcatcggcat tcggtagata
     2461 agtgttaatg aatatctcgt cattggctac atagccgcct gtcttgcgta tagtcgggag
     2521 aacttctgaa gtgacccaac gtttaaatgt tttagccttt ggtagcttac ttccgagaat
     2581 aagagaatac aagccagact cattaatgat agtcattgtt tgctttccac caaggacgcc
     2641 ctgaaatggg gcgtcatttt tatcatcatc atcaacgtgc cttgcaatgg catttcttgc
     2701 ctttgtatac cccaaaatct cagccacatc ttttccgaca aaccaaggct ctccgtcctt
     2761 aactattgtc ctcactgttc caaattcctt gtttgtgaat gttttaattc cgtccatttt
     2821 ctttgtcctt tctgttctta atttacattg ttgtttgaaa tttcctgctt acaagcataa
     2881 aaatacacta ttgtcaaaga aatagttctt gacagcagtg tttactcatg atataatata
     2941 tttaccagaa gtaacacttg tcaaaac
//
LOCUS       DAWEMR010000253         2929 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_11132_length_2929_cov_11.112039,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000253 DAWEMR010000000
VERSION     DAWEMR010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11132_length_2929_cov_11.112039"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          745 a          632 c          475 g         1077 t
ORIGIN      
        1 attgaaaatg gtctgcttaa cacagtatct aaatgagcta tatctgcttc atatgccgca
       61 gtcagttcct catattttgc aatccgttga ggatattcta ctgcaatcgc atcctgaagc
      121 cggaacagat tactttcata atttgctttc aacatcttta attttgttac ccgcacatcc
      181 agttccatct tttcttttat ctttggattt cccgttgcaa gtgccttcgt ttctgcataa
      241 gataatgcca ccgcatctac atcctgacaa gaacgtactg gcgatttgga ggtcatgatc
      301 tggctgatga atttctgttt gttttctaaa atctgccaca tatatgcatc aaaagtattt
      361 tccacaatat aacggaatat tttgactttt ttatttttat taccctgacg taagatacga
      421 ccttcctgct gtaggctaag tacccagcgc cttgccatat tgctatggta ggtttccaaa
      481 tactcgcccc cgaaccgtac ttacaactct cactgtatac ggctctccat ttataaactc
      541 aatttaactg tccacagtgt aatttcttat gacattctct acacaaagct attgttttgc
      601 gatttctcgc tatcataaga gcttcccaaa aagttttacc ttgtaaatct ttaagccgat
      661 gaacatgatg aatttctatg ggagtattag tttttccgca tagctcacat tctctggctt
      721 ttattctgtc gattagcttt gtactgctga aatatttgat tgtatttgga actaagtctg
      781 cattagattg gaatgctttt ttctgccgtt taaaaccctc gttataaaac actcgcattt
      841 tctcaactcc tttggaatta cggaacttta cagcaaaatc ttttcctgct ctgaattttc
      901 cgattacttt tgaaattgat gttctgtatt ttgtgccgta ggttttgtac atactgtatt
      961 ccataatata cttgtagctg ttgatgattg agctgttatt ggctatacag taataattgt
     1021 aaaaacctcg tatcatactg ttgtagtagt cgagtatttc cagatcatca cagtttttca
     1081 atctgtatat tgccttgggt ttccaaattt cttttccatt atggtagatt aacttcatag
     1141 cacctgtatc aatcaagtgt ttcctgattg tatcacttga aacttccagt acaattctgc
     1201 ccgtgtaatt acgaacgagc ttgcctgttt tatctctctt ggttagattg ctatgccgta
     1261 ctctgatttc ataactgaga aatttcgcac ttttttgggc attggtaatc agcgttttct
     1321 cttctgatag ctccaactgt aattgttctt tgagaaaatt cttaaaatct tcttttattt
     1381 tcttacaatc ctctttcgaa ccgataactc cgcacagcca atcatcagca tatctgacat
     1441 agaccagcct tgcataattg ttatccatag ggtcacttgg gggaatattc acccgttctt
     1501 tttccaactg ccttatgcgt gcaatcgcat tttctctttc acagtcgtcg tttgcatttt
     1561 tcaatgcgat tctggctttg cttaattcga cttcgttcct gcggtactct tttgtacgca
     1621 tacgcttttt acctttttta aattttcgga catactcatt tacatactta tcaaatttat
     1681 ccaaataaat gtttgcaaga ataggactga taatgccacc ctgcggtgtt cccgaatagg
     1741 tgttatgaaa agtccaattc tcaatatagc ctgcattcag aaattttctt accagtcgaa
     1801 gaaatctctc gtcattgata cgttctcgta aaattccaat caacgtagca tggttgatgt
     1861 tatcaaaaaa acctttaatg tctccttcga taaaccattt cacacccgtg aaagtttttt
     1921 gaatttcatt gagtgccgta tgacagcttc tattaggtct gaacccatga gaagtatttt
     1981 caaaatgccc ctcatagata gcttctaaaa tcattctgat aacctcttga agcaatttat
     2041 cctcaaagga cggtattccg agtggacgca tttttccgtt cttctttgga atataggttc
     2101 ttcttgcagg ttttggctga tagctttcat ctttgagcga aactatgatt ttccgtattc
     2161 tttcgatact cataccgtca accgtttgct tgtcagtacc ttttgtcata cttccgtcat
     2221 tggaataaat gttttgataa gcggttaagt acagttcctc attgtagagc aaacggtata
     2281 aacgctcgta cttgtaatct ttttggttac tgtgtttcgt taagctgatt aacacatttt
     2341 caggatttct cataatgtat cacacattat cctttctata ttgttaatgt tataaactgc
     2401 cttccttcgc catgtaagtg ccattaacac tctcggacta ctatgaaggc tccgttacca
     2461 tatcgaatat tcataagcct acacttaata gcttattttt aagcgtttcg acttaggtaa
     2521 tccccgttta gcatcatact aacgatttgg cttgttaggt tgtcggatac gacttccatc
     2581 cttttatgct tattgcatat acgtctcagc ctatctttgc ttacgctcct gaacagcgga
     2641 acataagtgc tgagatatag cgaataataa ttaccttacg ccatagtccc gacattgacc
     2701 gtttggacta tcgttcaacc agtatgggct ttatcctcat acctaacttt tcatctcccc
     2761 attcagtcgc agcttggtaa ttagctgact tatggcttta ccgacgtgct attttccctg
     2821 atatttagtt tccctcacag gtaaatcggt tgatgatagg actgtaagat actatcctaa
     2881 taccttgcca aaggtatatt tattatgatg cctttacggg cgcacgttc
//
LOCUS       DAWEMR010000254         2869 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_11398_length_2869_cov_9.111230, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000254 DAWEMR010000000
VERSION     DAWEMR010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11398_length_2869_cov_9.111230"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          781 a          683 c          503 g          902 t
ORIGIN      
        1 agatgataga acacaagggc aagcgtggca tttctcatgg cgacgttatc gaccttttca
       61 gggagaacat caaggatttt gacgtgagag aattttatgt agatatagta aaagagctta
      121 aagaacaagg cttctgataa aaaaacaccc gtatcgcaca tgatacgggt gctttgctat
      181 tttctggggc gaattaaata tttcattctg aaacgctgtc tgtccttgat agttttgcga
      241 ttgaagtttg ctatcttcgc attgaacgca gacatcattc taagctcatg ggttggaagc
      301 tcctcctcac cataaaaccg acagtctgac agactatcgg tcaaaactca gttatctatt
      361 tttgtcacat tgtcataaac tatcttatat ttttcgcaga ccttttcata atcctgctca
      421 tagcctgaca gctcaccgtc cctaagatcc tctgtaaggt catagctcgc cttgtaaagc
      481 tcatcaaagc caagattgct gcaaatgcct ttgagggtgt gagccatacg gaaagcttcc
      541 ttggcgtttt tctcctctaa agccttgcac atatcctgaa aagttgtatc atcagcaaat
      601 ctttttgcaa actttgttat cattttctca ctgccgaaac gtaccagaac gttgtcataa
      661 cttgcgccaa tgctttcata aaattctcta gtagtcatca ggacactcct tacaaattat
      721 atcatctctt attctacgcc ctttgcaagc tcgttctgaa tatctttaag acattcgaca
      781 agcacagggt tgaatgtgcc gcactctccg ttaagtatca atgccatagc cttttcatga
      841 gaatacgcct ttttgtaaac tcgctcgctg acaagtgcgt cataaacgtc ggcgacagaa
      901 actatctgag ccgatatcgg tatctcgtcg ccttttagtc cgtcaggata gcccctgccg
      961 tcataacgct catggtgcca cctgcatatg tcatgggcta tcttcaagag tggctcgtca
     1021 cggaactgct caagactttc aagcatattc gctccgatag cggagtgagt tttcattatc
     1081 tcaaactcct cttttgttag cctgccgggc ttattgagta tcttatcatc aatgcctatc
     1141 ttgccgatat catggagtgc cgcagccatt ggtatgagaa gctgctcagt gccgctgaca
     1201 gtatacttgt ttgtgcgcat aggaagccta tctaaaagca gttccgttat gcgctttatg
     1261 ttcaccacat ggcttccact ctcgccgttt ctgaactcta ctatctggct gagtatgctt
     1321 atcatcatac gattgttttt atctttttcg tacatctggt cgctgacaag ggaaataagt
     1381 ctgcgctgtt ttgcataaag cttaatggta ttgaaaacac gctgatacac aacgcgtgaa
     1441 tcaaagggtc tgctgatata atcagaaacg ccaaactcat atgcttttct tatagaggat
     1501 gtagaatcct cgcttgatat cattataaca gggatatcat ctatccaatg atgttccgcc
     1561 atatagtcaa ggacttcaaa tccgtccatt tcgggcatga ttatatctaa aagcacaagt
     1621 gaaattccag tgccatactg attaagcagg tcaatacact cctcgccgct tgccgcctca
     1681 tgtatgatat attcattgcc aagcatttca gagagtatct ctctgtttat atttgaatcg
     1741 tcaacaataa gtatctcctg cttttgaggc tcaccgacaa cactttcgtg accatctgtg
     1801 acaactgtat ctttctttgt ctttgcctga tacataagct tatcagcctt gctgacagct
     1861 tcgtcaaggc tattgccatc agagaaaacg ccgccgacgc ttgctgacag acgtatattt
     1921 gaataccctg gcacagttgc cgcatggata tttcgtttta tcttcctgag cttatgggca
     1981 aaatcatcct cctgaatatc aggcatgaca agcaaaaact cgtcgccacc ataacgcaca
     2041 agcacatcag attttctaat acagcgcctt atagcatcaa ccactgtcac aagcaccata
     2101 tctcctgcgt catgaccgca tgtatcatta taaaccttga agtcgtcaag atctatcatc
     2161 gcaacgcccg caggcacatg ggacttttta agcttatctt caaaatatct tctgttgctc
     2221 gcacctgtca acgcatcagt atacattgcc ttatgatatt ttgacagctt attcattatc
     2281 ttttctctgt cctgaacacc cagcagagag tcctcatcaa gctttttgat aagctccata
     2341 acatacggct ctccgtcaat ctgaacatac tttgaaaaga cctgataaat gtcagttccc
     2401 ataaattcaa gcttagttct cggcttttta tcaataaaag tttttaagga tatacagttt
     2461 tcacaaggct cgtccacttt ccaaaaagaa aaacactgac atggattttc aacgcagccg
     2521 ctcactccat gaggaccgtc cgctttcaga agcctgacaa aatcaaaagt tttcctgaga
     2581 acgtcaattt ccttttctat ctccgaagag gtagtactca gttggctttc gctgttatca
     2641 tcgcaggtcc ataatttgta cacgtccata ttcacgttct ccttacgctt agtgtagact
     2701 tattgttgct ttttttactt ttttcgacat tattaactga tttattatac cacacctcaa
     2761 tcacattgtc aaccaaacga tacaaattat aatattattc ataatattaa cacattcata
     2821 ttttaagcaa ctttgctcta gtttttcata tatttatcgt cattcataa
//
LOCUS       DAWEMR010000255         2854 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_11458_length_2854_cov_9.501965, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000255 DAWEMR010000000
VERSION     DAWEMR010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11458_length_2854_cov_9.501965"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          856 a          571 c          601 g          826 t
ORIGIN      
        1 acagcacaca ttttgacaaa tcaatgaagg taggagcaag tgtagtttgt gcaagacgag
       61 gcggctgttc ggctacattt gatgagctta acaagtattt cactatttgc aatatgccta
      121 ttgcctcaag tcagtattgg aacagtattc acggacgtga agtaggcgag gctgaaatgg
      181 acgatgaggg taaacagaca atgcgtgtac ttgcaagaaa catgactttc cttatgaaaa
      241 gcattgcact tggcaaagaa aaatacggct tacctgaggt cgaagaacac gcttggactc
      301 attttatcag ataactctat acataatgaa aacatgaaaa atatccgttc tgcaaaaaaa
      361 cagaacggat attttttagt tatatttcat tcaggcactt tgtcactcgc ctgcgcagtt
      421 cttctcctcc aaagggaaag ccgaactctg cggcggagct tccccactca ggtgggtgct
      481 tctttgagcc gtcaaaatag cggcttttca ccacgccacg ataacgtttt ggcatttttg
      541 ccgcaagctc ctcacattct ctcaagcgaa gctgcacctt tgcaaggtca agccacaggt
      601 cggtgagttt tttccctctg tgggctaccc tgccgcaatg ctcccccact gcggcaagct
      661 gtgaggacat ttcgcagata tcttctttaa gcttccactc ctgccacctt agtttgcgat
      721 actgtgaaag ctttcgcagt gcggctgtat taactctgct tccaccgctt ctaaaattat
      781 atcggctcgc tgagggtgca agccttttta tggaatttac ctctacggaa taatctatat
      841 tatcgtacat ttttctcctt tcgccttttt gtacagacat ttggacagtt agtatgatat
      901 actgtaaata aaataaagca atgacatttc agataaccgt tttctgtata tctctcgggg
      961 cggagatgtc aaaaaaagga gggggcggtt tatcctcttg acttttacag taaaatgtat
     1021 tataattata tcaaagccag attttatgca gatatctgta ctaacgatac atacagtata
     1081 ctacaggttt ctgcaaaagt caatatctat cagcaaattt gatacagact tctgtatttt
     1141 tgtgaaatga tcacaattgg aggaagaaaa tatgactgaa atgtacaaaa ggataaaaga
     1201 gctatgcgag gagaaccata tgacggtatc gcagatgtgc aaggagctgt ctataacgag
     1261 gagctgttta tctgagctga gcaaaggcag gacggaacga ctttccgcag agaattctac
     1321 aaagatagcg aacagatttg gagtgagcgt acaatatctt atatctggca gggaagaaga
     1381 ttttgacacg aagctgaaat atgcattatt caacggcacg cttggagtga cggacgaaat
     1441 gtttgacgag gtcaagcagt atgcagaaat ggtcatgctc agagaggaag caaagcgcag
     1501 aaaggctggt gaaaagtgag agatgacgaa catacaggaa ttgtatgata tttgcagtag
     1561 agagaacata actatagatt ttattgattg tccaaagtgc ggggcgatat cgctttttgg
     1621 cgcaggtggc tgtgcgatcg gcatagacag ccagacgcct gcaaggcttt tgaaggagcg
     1681 cactgctcac gagataggtc actgcgtaaa gggtgcgttt tacaacagat acgctacggc
     1741 agacaacatc tcacagcatg aataccgtgc agacaagtgg gcgatagaaa atctgatacc
     1801 gaaaagcgag cttatcaaag ccataaagaa aaatctggac agtatttatc tacttgcgca
     1861 acattttgat gtgagcgagg attttatgat aaaggcttgc aggtattatg gggtttattt
     1921 tgggtagaaa aatgtatata ccgcccacag aaccgcaact gcactgactt aaaaccgcac
     1981 tttaacggtt acaaaaccgc gctttcgcca ctattgaacc gcacaatata tccacgaatt
     2041 tttcgctgac tgcccacggg aagtcagcaa caagttgtgg caaaacgtcc gtgcagttgt
     2101 ggcagtcgaa gcgatatata caaatcaagt gctaattgtc ttgtggcttt tatccaaaaa
     2161 caaataaatg gttgctgccg atgtaattat tgttgtgaat atggcaatga gcactgttat
     2221 atccatttcc ttgttttcat agcttaattg cactgtataa ttacccttag gtataactgc
     2281 tgaaatatag ccatctgagg tttctgacag tttagcatct cctactgatc ttccgtcttg
     2341 atataatgtc aatttgtaat tatcaacgta ggtcattgat acaactatct cggtatcctt
     2401 agcaaagtca gcatcaaaat ttagcgtatc caaatctgct tttattggaa tttcaatatt
     2461 atccccatag gaacagatag gagagactcc gtctatttct ataagctcca taccacgcgt
     2521 ccaatcatgt acacttgata tcgtcaattt agggcatttt tcaattactt gggtgaatgc
     2581 ttcaatagct ttcggactat ttttatcgac cagaacatat tttactccgt ttgatatcat
     2641 ctgatcttca aacttattta tccattcggg gtctttcata tattgcagat aacttaatgg
     2701 tgatgctacc atattgcaca tcataccttc atctgtgata ctgctcatta tcgcattgct
     2761 ctgatcaaag cctttcttgg aaaatatatt atcataaccg tttaaggaaa acaagccata
     2821 ctcagtgctg atattttttg taaaaccata gaaa
//
LOCUS       DAWEMR010000256         2815 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_11637_length_2815_cov_8.476812, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000256 DAWEMR010000000
VERSION     DAWEMR010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11637_length_2815_cov_8.476812"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          762 a          581 c          524 g          948 t
ORIGIN      
        1 gttttccatt tttcagggac aactctcctt gcatctgaga gataaatttc atggtgtaac
       61 cttgtcttag ttatgtcatt gacatatccg tttttttcta gatattcatc aattaaagca
      121 atagtagctg gttcatcatc aaacgaaccc aagtgcatta tttgaacaca taatccctcg
      181 tcaattgtca ttagctctgc tgacgaacaa tctaactttt tctttttagt tgctgtttca
      241 actgcccatt caaagtcttt ttgagttata aattcaggta aacggataac agatatccaa
      301 ttgaatgatg ccttatcagt atagtctata ccgtctatgc cgtcctgcca ccaaaaaccc
      361 tcaagtggtg gaactacata ttcaaaaaat ccttcaattt tatgatctgt tttataactc
      421 attttcagtg tataagcaac agcatatagc acactaattg cttgttgata tgctccacat
      481 acttcatttg ggtcgccttt tcctctgatt gcaatataat ttgctttagg aacagttaca
      541 atttctggct tgtttttagg catataaaat tctttgtact ctttcttaaa atcaaaagcc
      601 atgattacct ccataaaata taatctataa atacgcttta atacggcata accccgtttt
      661 gtcctcgtcg gttatgttat taggacaagt atatcacatt ttgaagtgat tttcaaggga
      721 ctgttttatt ttgtcaacat aaaactccct gtctgtttca aagcagtatg cgcacagacg
      781 tccacctgag gcacagccac agtctatggc tatgtgtccg ttggctttgt aggcttcggg
      841 tctgccatgg tttgggatat tggcggtagg ggtgtgacct gtcacaagga aagtattcgg
      901 gtcgctgaaa tagcgttttg aatagtcggc acgctcccag ataagctggt ggagggtgta
      961 ttcatcaagt ggcttatcct cgctaaagcc cccaagacct gcatgggtga ggacaaaacg
     1021 tttgccgttt atggtgatat cctcatacat tgagaagctt ttaagaaact ctataaagtc
     1081 cacacggtcg gctttggaga gggaaataaa ttcgctgaaa gtgggtttgc ctccgctggt
     1141 gacccaatcg atcaggtcta gccgttcgtc ttttgtaagc ttgctcacat cattttttat
     1201 gaggtggcgg agattgatat aggcaaggtg ttcgtggttg ccgaacagcg ggataacatt
     1261 atcatgagcc atgatgtcgt tatagatctc cataggcttt tcgcccctgt cgcagatatc
     1321 acctaggata tagagggtat cgtcctcaga gaagcctatt ttttcgagaa gcttaatata
     1381 ctcctcataa cagccgtgga tatcagatat tgcatatgtc ataaaagcac ctcgctgggt
     1441 tttgggtcgg gtttcttgtc tattctatta tagcatactt aggggtaaaa ttggggaggt
     1501 ttgaaaaaaa tttttaccca ttggcataga gttcgcttca agctgtataa tggggataag
     1561 ctcatggaac tcttttggct tatgttatct gagtgaaccc ttggtgctta taggcgttgt
     1621 caccaccgca aggtctgcca cgctgctttt cagtgtggcg agagcctgtg gtgttgtgtg
     1681 gccattgttt tgataataat ttaactgtac ctttataaac tgaaatttgt cgaacgaaag
     1741 aaaggttttt gttgtttact gatcatgtaa aatcattgct caaatatgca agcgtacttt
     1801 ggatcttctg taattaactt cttttccttc tctggaaagg aagtcgcagt ctttactaaa
     1861 aagcttggct cacatacaat tactttatga cctgacggat ttacagattt caacagtgat
     1921 gaagtaaaat gctcatcgtt attctcatca tttatgctct tattattccg ttcttcccat
     1981 cttttggatt gtttaatttt ccattgttca aatttaataa attgctcaaa cgctgaatca
     2041 aaacagtcca ctgcaacttt atgatctccg atattatttg cgattttgac gcatctcata
     2101 tctataaaac ataagtcact gtgaaatcca tggcattcag tacctactat tttatcaaac
     2161 aactgtcgag tcaataaaag aagctccaat ccttctcgac tattattcag ctcattatta
     2221 cacactatcg cttcatcaag tgatttttga aaagcatgaa gaagctctaa cactgcgtct
     2281 ccacgatatt gttcagccct tttctcatca tttatactgc cgatactccc taaatttcct
     2341 aacagaactt gtctgcacaa ttcaaggtcg ggctgttcag atgctttctg ctcggctttt
     2401 tcatatttac caaggcagga atatacggat aataacggaa tgatacacga ttggtcatga
     2461 gacaataatt tctcatacag tttagcggct tcttcccaaa agatatttgg ctctttgcct
     2521 ttatttttcc aaccttgctt atgtaaagca caagcaagct tggtcttaaa atccactctg
     2581 tctggaaact cttcaagccc tctacgcatt agagcaatac actctgtcat atcatcgtta
     2641 ttatttatca gccattgagc cttatcatta tattcctgaa taattctgtt cctgtcatta
     2701 ttatagccaa acaattcatc gatcgatata tggaaatagt tagctatcga tgggatcatc
     2761 tccatgtccg gataaccgcc atttgcctcc caccttgaaa tggcttgtgg cgtaa
//
LOCUS       DAWEMR010000257         2815 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_11636_length_2815_cov_8.537319, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000257 DAWEMR010000000
VERSION     DAWEMR010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11636_length_2815_cov_8.537319"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          647 a          717 c          578 g          873 t
ORIGIN      
        1 gaataataat agatataaaa aacgcagccc ttgcagactg cgtttttacg ttatataagt
       61 cttttgatta tagtgatgtt gttgtatctt gaaaaatact caacatttga aataacgata
      121 ccagtggcag tgttctgggc gtgtaccatt ttgccgtcgc cgatatagat agcaacgtgg
      181 taaatgctcg agatcgatgc tccgccaaag aagataacgt cgcccttctt catgttgtta
      241 tatgaaactg tagtgccgta taaagcctgc gaagccgcaa agtgcggaag atttattccc
      301 acctgtgcga acgagagcat cacaagaccc gaacaatccg ttgcaccaaa ctgtgaaccg
      361 ccccatatat acgctccgcc caccatgctt ttggcatatg aaacaacagt gtctatttta
      421 gaactgcttg aataatcagg cttaggtgca ggcgccgctg ttgttgtggt agtggtcttt
      481 ttgcttgttt caggctttgg cttagctgtt tcctgcggag ttgtggtagt ctgaaccgta
      541 gtttcgtcag gcttttccgt ctgagccggc ttcttcgtgg tagtggtctt ttcaccgctg
      601 ttgccactct ttttggtggt ggtagtcttt ttcttggtgg tgatcgtagt cgtggtggta
      661 ttcgcctttg aggacacctc cgaatactta ttgtccacct gctgtgactt attttcaagg
      721 tcgctcttat cgttctttat ctgctcgata agcttgcccg actcctgctc cattttcaga
      781 agctccgcct gctcgcccgc caaagttttc tgctccctgg catattcctc agcctttgct
      841 ttgagctgtt ttatctgctc atcaagctcc gccttggtct tttcaagctt tttatactga
      901 tccaaaagtt cgttaagttc gtcattatcg tgctttgcaa ctctcgtcac aagctctatc
      961 ctcataagaa tgtcataaaa atcagatgaa tttatcagca cgttcgtgta cgaatctgct
     1021 cctcctgcaa catacattgc acgaatacgc tccataaaat cgtccttgcc ctcgtttatt
     1081 gcagtttcct gcaagtcaag gctgtgctga gtgtcaagct gtttgccgtc aagctctgcc
     1141 atttgatctt caagctcaga ggaatatttc tctgaatttg ctatcttttt ctgtatggat
     1201 ttatattttt tctttatagc ctcaaggttg tcctgctcgt cggcaagctt tttgtcagcg
     1261 tcagctatct gcttatcaag ctcctgctgt tgctttttta gctcttcaag ctgactgctg
     1321 tatgtatcgt tatccgccaa agcgatatca cttacgcttc cgtcagaaaa accaaaggcg
     1381 gcaaaagcta tgcacagagc catacagcct gcggctattc ttttcatggg gtaaattttt
     1441 ttcataatat cagttctcca aaatataagc gtattatata tttctcacgc tgtaacgttt
     1501 atgtagtccc tttaattata acgcaaaacc ccgttcttgt caagaaacca accgctaaaa
     1561 gccgtatttt cggcatttta agacaattcg ccgcacatat tttgtgcgtt attgcaccta
     1621 ttctcctccg aacatctcac ccaaggtctt tcctataagc tcgcctgtat actgtgcctg
     1681 agtgatagaa ttgccgtggc ttcccacctc tataagaagc gagccggtgg tcaagtcctg
     1741 attatagtgg cgatagtcga aaagaacagg ccgtgtaagc ccctgccaat cgctctccaa
     1801 agccgactgc aaagcacagg caaagtgaaa attcttcata tagtcaggca tacccatagt
     1861 gccgtcatca caccctgata ttatcataag ctgagccgct tctctgccat ctatctccgc
     1921 cacaggtgcg agccttgtgc cgtcctcacg ctctatgccg tcacggtgaa cgtcaagaca
     1981 tacctttata gaggggtact cctccaatat ctgctttacc gccattcggc ttgaatcgta
     2041 agagccgtca tagctgggat aatcatgcac aagtgtatcg tggatatacg caacgcctgc
     2101 tttgtcaagc tgttcgcata tcttgtcccc taccgacagc atattcattt caggttcggt
     2161 ggtcttgcag gaaaaggagc tgtcatagtg gtctttgtct gtgggctcaa agctttccgt
     2221 ggcgtgggta tgataaataa gtatctgcgg ctcagaggaa tttccgtcta tttgaaaatc
     2281 atatggcttt gagctttcct cgtaaagctc atcatttgaa aggtcagtgc agtttctcac
     2341 ctgaccgccg ccgtcaaggt cgaaatactg ctcgccgtca tatcttccgt aggtgtattt
     2401 ttctataaca ccgtcattcc cctccatgct tgcaggatat ggtatcttgt ccgtcacgtc
     2461 ctgagcaggc aaaggacttg gcacttctgc gcttacagat ggcgtttgct tctcctgtgg
     2521 cgccatatcc atatcccata tgctgtgctt tgacaccact gccgcttcaa gcttgtttga
     2581 taccacaggc tttgatgccg tatccgtttc gctttccata gcgaaacaag gcttgtattc
     2641 tcccatagcc acccctgccg ccacagccgc caccctgccg aacctgcttg cagatcttgc
     2701 ataaagccat atccccgtgg gcataacgaa cgccactatt ataactgcaa cacaacgcag
     2761 gcagactttc tttatatcca atgctgtcac tcccatatac atcttagtat tataa
//
LOCUS       DAWEMR010000258         2794 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_11722_length_2794_cov_11.354874,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000258 DAWEMR010000000
VERSION     DAWEMR010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11722_length_2794_cov_11.354874"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          812 a          564 c          709 g          709 t
ORIGIN      
        1 ttaaagtgca gcgtccgtca aggtatatcg gcggcgaggt gggcagcgtt gtaatggata
       61 aggataaggt ggacgtgcgc tttgccttct gctttcctga tacctacgag gtgggtatgt
      121 cacaccttgg catgaagata ctttatggtc tgaaaaatgc ccgtgaaaat tggtggtgcg
      181 agcgtgtttt catgcctgag gaggactata acaagcttat gcgtgagaac gatatccctc
      241 tttacgctct tgagagcctt gaccctataa aggattttga tttcatcggc tttacgctca
      301 tgtatgagct ttcttataat aatgtgcttg aaatgctcga ccttgcaggc gtgccggtgc
      361 ttgcaaagga cagaactgag cttacacctc ttgttatcgc aggcggtccg tgtgcctgca
      421 accctgagcc tatggctgac tttttcgacc tgttcattct tggcgagggt gaggaagtca
      481 acgttgagct tcttgaactt tacgagagaa tgaagccttt gaagcctact aagcaagagt
      541 ttctaagaga agccgcaaag ctggagggcg tttatgttcc gtctttctat gacgtggatt
      601 acaatgagga cggcacagtg aagtctatca cgccaaacgt ccctgaggca cctgctaggg
      661 tcaaaaagcg tgttataaag gactttgaca aggtatatta cccagaggga tttgttgtgc
      721 cgttcactca gatagtccat gacagagcgg tggttgaggt actgagaggg tgcataagag
      781 gctgtcgatt ctgtcaggca ggctttatct accgaccttt cagagagaaa agcatcgaca
      841 ccataaagaa cgaaactaag tccctttgtg agcagacggg ctatgaggaa gtttctcttt
      901 cgtcactgtc aacgagcgat tacacagaga taaacaagct tttggaaaat cttgtggaat
      961 acactgacgg cgagcagata aacctttcac tgccttcgtt gagaatagat aatttcagcg
     1021 aagagctttt agagaaaata aagtccgtaa gaaaaagcgg tctgaccttt gctcctgaag
     1081 caggcactca gcatctgcgt gacgtaataa acaagaacat caccgaggag gagataatgc
     1141 gtacctgtcg cactgccttt gagggcggat acagcactgt aaagctttat ttcatgcttg
     1201 gtctgccaga ggaaacgctg gaggacgttg agggtatcgc aaagcttgca cagaaagtcc
     1261 ttgaccttta ttacgaaaca ccaaaagaaa agcgtggcaa gagtgtgcag atatcagttt
     1321 ctactgcaac attcgtacca aagcctttca cacctttcga gtttgaaccg caggcaactc
     1381 ccgaggagat aaaagagaaa caggaacacc ttttcaaaag tcttacaagc cgtaagatac
     1441 gccttagcac tcaccaatcc tatacatcta tattggaagc agttcttgca aggggtgaca
     1501 ggcgtcttgg taaggcgata tacaccgcat ggaaaaaggg ttgctatctt gacggctggg
     1561 acgagcattt caaggctgac aagtggcagg aagcatttga ggaatgcggt cttgacacaa
     1621 gcttctatgc tcaccgcaga cgttcttatg acgagatagc gccgtggtcg caccttgatt
     1681 atcttgtgag ccacgagttt ttcgttcgtg agaacaagaa agcacatcag gcagtgacta
     1741 cacgaaactg caaagagggc tgttcaggct gtggcataaa atgcgaatac ggaggtgttt
     1801 actgtggcaa acgctgagcc gaagaatgta aatctccgtt tggagagatt tgaatacaga
     1861 gcggtatttt caaaaacagg cagggcgaaa tacatttctc accttgacct tatgagagcc
     1921 tttcagcgag tgttcaaaag ggcgcacctg cctatatggc atactcaggg tttcaatcct
     1981 catgtgtata taatgttccc gcttgcattg ccccttggca ctgacagcag ggtagagata
     2041 atggactttg ctctcactga ggatatccca tacggcgagg ttttggagcg aatgaatgca
     2101 caaacgcctg ttggtctgga gatagtgagc gtttcaaagc ctgagcataa gcataccgat
     2161 atcgctgcgg cggagtatgt ggcaaggctg aaaacagata agagcgtaca tgaagtggcg
     2221 gagcttttcg gcaagttcat agcacttgac aagatagaga tagaaaagcg aaccaagaaa
     2281 aagactatca atcttgtgga tataaagcca catatcacag tgcttgacag caaagagcta
     2341 gaggacagtt ttgaggtgac attaaagctg ccgtgtggaa gctccttcaa tcttaatgtg
     2401 aacgtagtca tagacacgtt ctgtgagcgt tatggtatag aaatatcgga aatttataca
     2461 gaacgtacaa aaatactcat ggcaaatggc gaaaatttta tataaatatc cttgcaaaag
     2521 gttttgagat gtggtataat attaaagcat ttgaattacc gtcggttctt ccgacgagag
     2581 ttaatgccac gcctttgaac gaaaggcacg acattaaagc gggcagtcct ctggctttgc
     2641 agcgatagct gccataaagc gaatgaatgt ctgaaaatat taaggaggaa tttaaagtgg
     2701 acgctttaaa gcagatttct aattcaagcc ttaaatccga ggcacctgtt atcaatgttg
     2761 gtgatacagt aaaggtacac gtaagaatta caga
//
LOCUS       DAWEMR010000259         2759 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_11859_length_2759_cov_12.533654,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000259 DAWEMR010000000
VERSION     DAWEMR010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11859_length_2759_cov_12.533654"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          697 a          699 c          558 g          805 t
ORIGIN      
        1 tgagcttcat ctcatggata cactcaaagt atgcgtttct tgggtcataa ccagcttcac
       61 aaagtgtttc aaaaccagcc tgcataagtg cgcaaacacc accgcagaga acagcctgct
      121 caccgaagag gtctgtttct gtttctgttc tgaatgttgt ctcaagaaca cctgctcttg
      181 ctccaccgat accaagtgca tatgcaagtc cgatatcctg tgcgttgcct gttgcgtcct
      241 gctcaactgc gataaggcaa ggtgtaccct taccagcctg gtactcagaa cgtactgtgt
      301 gaccaggagc cttaggtgcg atcatgataa cgttaacgtt ctttggtggt acgatgcagc
      361 caaagtgaat gttaaagccg tgtgcgaatg caagagtctt accctctgtg aggtatggct
      421 cgatagattt cttataaagt gcagcctgct tctcatcagg aataaggatc atgatgatgt
      481 cagatgtctt tgcagcttcc tcagttgtca taactgtaag accctgctgc tcagcctttg
      541 cccagctctt agaaccttcg taaagaccta ctactacgtc aacaccgctc tccttaaggt
      601 tgagtgcatg agcgtgaccc tgtgaaccgt aaccgataat ggcaactctc ttacccttga
      661 gtacgttaat gtcgcaatcc tgctgataga atattcttgc ctgtgccatt gttaattcct
      721 ccaaattcag aaatttaaga tttattttat gtagcctttc ggctatataa tcaaagttta
      781 tttgcctgca ttgaatgtgt cagagccttt ctctattgcg atagtgcctg ttcttaccat
      841 ttcgattatt ccgtaaggtg ctatgacctc ctcaaatctg caaaggtgag cgtaagtgtc
      901 gcaaagctcc aatgtcatcg tcgtaagcgt tatatccatg accttagccc ctgtgatatc
      961 cgcaatagtc attatctcac tgcgtctgtt tgcgaccgca gacaccttga ctatcataag
     1021 ctctcttgcg agatactcat tagcctcaaa tgtctttacc ttgatgacct ctataagctt
     1081 attgagctgt ttctcgatct gctcaactgt atgctcgtca ccgtcagcaa ctatcgttat
     1141 tcttgaaacg tcagggtcat gagttggacc tactgcaagg ctctcgatat tgaacccacg
     1201 tcttgaaaac agtccggaaa ttcttgatag aacaccgctg tggttttcta caagaactga
     1261 gatcgtatgt ttcataaatc aacctccata aggaaacctt tccttgattt cttattatac
     1321 cagcttatta ttaagatttt ttgaacgtaa acactatata ctgtaaacgc tacagatagt
     1381 taaaatattg gccttataat gttggcatat ccttatccac aatgacttca acaaggcaaa
     1441 tgcctttatg ttcagcaagc atttcgatgc ccttatccat agtatcatcg tcataaactc
     1501 tcattgcagg tatgccataa gcttctgcga gcttgataaa gtcaggagag ccgtcaagaa
     1561 aaactgctgt ctgtctgtca tgatagccat ttgtctgaag ttctcttacc atgccaaggc
     1621 ggttgtttgt cattactacc acctttatat caacgccgaa ctgaacggct gttgcaagct
     1681 ccatgaacga catctgaaaa gctccgtcac cgcatatagc aactgttgct ctgctgttgt
     1741 cagccatttt tgcacctatt gccgcaggca cagaataacc catcgtaccc atgccgcctg
     1801 atgtaagaag tctgccctct ttgcggaact tatattcagt gcaggtccat atctgatttt
     1861 gtccaacgtc tgccacaaca gtaacattgg caggcatttt ctttgcaagc ctgtcgataa
     1921 acaccctcgg attgacagta ccctttgcag gctcaacata ctttctctcg cccccaaggg
     1981 tcttgatgtg agctatccac tcatcgtgct tcataggctt tgcaagctcc gtcatttgct
     2041 gtaaaacaag ctttgcgtcg ccaacgagag gcaggtcagc accgatattc ttgcctatct
     2101 ccgcagggtc gatatctata tgtattatct tagtagtttt ctccagagcc aaaggtgttt
     2161 taacggctct gtcgccaact cttgcaccca caaggaacag cacgtcgcac tcgttcacag
     2221 cggcgtttgc cattttaaca ccgtgagttc ctatcatgcc gcagaaaagc tggtcgtcat
     2281 cagggaaaac agaaatgccc atcatagttg tgataacagg tatctgcata aggtcggcaa
     2341 gctctcttat ctcgttctca gcgtgagcgg caaaaacacc tccaccaata cagataagcg
     2401 gcttctcagc ctcttccagt gccgccatta cacgccttac ctgaacgtag ttgccctttg
     2461 tggtaggctt atagcttctt atctcgcact tttcagggta ctcaaagtcg ataacagctt
     2521 tctgaacgtc catagggata tctataagca caggacccgg tctgcccgtg cctgcgatat
     2581 agaaagcttc cttgaacact ctcgcagtgt cctcagggtc tttgagaaga tagctgtgct
     2641 taacgaacgg ctcagccgag cctgtaatgt cagcctcctg aaaaacgtca gagcctatct
     2701 ggtcggtatt cacctgacct gttatgcaca cgataggtat agaatccgca taagcggta
//
LOCUS       DAWEMR010000260         2704 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_12121_length_2704_cov_21.271046,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000260 DAWEMR010000000
VERSION     DAWEMR010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12121_length_2704_cov_21.271046"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          736 a          593 c          630 g          745 t
ORIGIN      
        1 ctttttcggc acagtacaga ccataggcga caacacagcc gagataaagc tcacagacgg
       61 caagaccgtt tgcacagtgc ctacatatta ttgcgacggt gagcttagtg aggggcttga
      121 agttatggtg atacttgact gctccacaga tcttttcggg agcggaaagt cggagagttt
      181 tgattacgcc gttatctgca ccgaccctgc ggagtatgac ccctcagggc atgagtttgg
      241 aaatctcgcc tacgccaaag ctgacagcaa taaccttggt aggtttatct acaccgagat
      301 cggagatata gagaaatgaa actaaaactt ctaaacacaa taaaatgtga cctgaacaaa
      361 agcataataa acataggctt tgcaggggcg gtgctgctga cgacagtgct tgctttcacc
      421 gcctcggcct atacagacct tgccaccgat aaatcatatt cggtgttcga gtcacttttc
      481 accctcgata aaaatatcct ccgcaccgac ccaatgctgt cctcagtgct ggttttcaga
      541 aacggacttt cgggatatat cacaatgttt ttgcctattg tcgtggcatt cccattcatg
      601 gtgtctttct gtgcggagcg aaataacgga ctgatgcgtt tcaccatatc tcgcacaggc
      661 aagctaagat attatctttc aaaattcata tccgcactta tctcaggcgg atttgccgtt
      721 atgctaggta tagctgttta cggcatagcc tgtgcagtgc tttttcaacc actttcggag
      781 tctgacgtat cagccgacca gcttcaatac attatccaag acagcacagg taaaacggta
      841 gtcaaaatgc ttgcggtagc tttcgcatat ggtgcagttt ccacactgcc tgcgtttttc
      901 ctaagctctt tctgcaaaaa tccgtatatc ataacctgcc tgccctttat gctgaactat
      961 gtactcacca caatgctaaa caggataata gacgcaaatc ttttcaacga taactttgat
     1021 tttgatagag cgaatgcctt ttatccaagc tctgtgacga attttctgta tatccaaagc
     1081 tttgacagaa gcgcaaagtg gataactgcg gtgaactgtt cgggggcgat cgttgccctg
     1141 ctgggattta ttatggtgat gagtttgcac aaggacaagg gggtgtaggt cagctatgat
     1201 gaaaaatttc agcctaaaac aaagcttttt ctgtgcaagg gcggagttca taaagtgggt
     1261 atgcgacgca cgaatgataa tcctgggagt gcttcttatc ttcatttaca gctttgctat
     1321 cgagcctctt aaatcaaacg cagagcttat gggcgaaccg ctgaatatcc ttgagccttt
     1381 tatcgccata gctaattcag gtgcgatact tcttatcata ccgcttgttt ttcttacact
     1441 tatagcagat tttcccaaga tagacaccaa cacagttttc tacataatgc gtgtgggcag
     1501 gctgaactgg ctgttcggac agcttttgaa gctcattttt atggcgttat cttatcttgc
     1561 agtgatattc ttgggcgcag tgcttccaat gctttcagac ggcttttggt acaatggctg
     1621 gagcaatgtg gcgacaaagt ttgtttcacg ctttcctgaa cattcgggca atttcggcgt
     1681 acagcttttg cccgaaaacc tgtataatca gctcacagta ttttcagccg ccgtgcaaag
     1741 ctatctgctg gtgttcgcat atctcatgat aatagggctt atcctgctga gcttttcact
     1801 tgtgaaacac aaaactgtgg gttttgtcct ctgcggagcg gtgatatctt taggcacggc
     1861 gttctgctcc ataaagacca cgcttatgtg gacaatgcca atggcgaaca gcataatatg
     1921 gctgcactac acaaaatatt tccgtgagcc tgttatgtca atgagttttt cggtcagcta
     1981 tcttgccata tttatagcgg tactgctggc gttctgcttt atcgccataa gaaaattcaa
     2041 ttacgacaat gttgctgaga tagcaagtta gggattaggg ggacgaaatg gatatcatca
     2101 atgtaaacga cgtatgcctt acgcttggca aaacggagat actaaagcac ataaatgtgt
     2161 cctttgagga gggcaagata cacggactta tcggcagaaa cggcagcggc aaaacgatgc
     2221 tgatgaagtg catatgcggc tttatcaagc ccacgagcgg tgagattatc gttgacggca
     2281 agcaggtggg caaggacgtt gacttcccga aagatatggg tatcattatt gaaacacctg
     2341 ggtttatacc gtattacagt ggatacaaaa atctcaagct gcttgcaggg ctcaacaaca
     2401 agatcggcaa agatgagata aaaagcagta tgaaacaggt gggacttgat cccgaactaa
     2461 agcgacacgt ccgcaaatac agtctaggta tgcggcagcg tttgggactt gcacaagcca
     2521 ttatggaaaa tccgaagata ctcattcttg acgagccttt caatggtctt gacaaggacg
     2581 gcgtaaaaga aatgcgtgag tacctacttt catacaagga gcagggcaag actattctta
     2641 tatgctcaca ttcggcagag gacatctccg tgctgtgcga tactgtgcat gaaatggata
     2701 aggg
//
LOCUS       DAWEMR010000261         2663 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_12321_length_2663_cov_8.472009, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000261 DAWEMR010000000
VERSION     DAWEMR010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12321_length_2663_cov_8.472009"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          815 a          544 c          462 g          842 t
ORIGIN      
        1 aacagaactt ggaagccaaa tgtaaagaga gttaaagctg ttgtaaacgg cactccttgt
       61 cacatttacg cttgctcaag atgcctgcgt tcaggtaagg tagaaagagc ttaatgcttg
      121 aatattccaa aaaatcgact agcttcgcta gtcttttttt tatgcttttt aagatacaac
      181 aaaaaaacgg agccgagtgt catactctgc tccgattttt attatgcagc gtgtctgcca
      241 aatgttctct tattctcagg cggtgcaaaa acagaattat cacctgacga tgaattgtca
      301 ctgttcatat tgttgttact gaaaccgtgg tcggtcttat atatcatatc atagtagatt
      361 atagcattgg aaatgttaat tcgctcctcg gcataatttg aatagttact gatataatcc
      421 tcgtccacaa caccatcgtt tattcgtgcg ttacaagaat cctgacagta cgtttcattc
      481 acagtggtct tgaacagacc ctgattgtcg tccatataca ctgggttagg atcatcttta
      541 tatacttgat tgcatgacac cgccgttgcc acatttttgt aatccgtaag atcaaaatat
      601 gtgtttttct ggctatcata gtaaacagta tctgttacaa aattgccgtt tgggaagata
      661 acaacatttt cctcgttatc agcaacagaa aagatatcat gaccaagtgc atagacattt
      721 gagaagccaa acatatttcc aagtgttggc tgaacgtcat acatacccat tatctgcttg
      781 acctctgtag gctcatatcc gccgtctttc gaccatatta tgaacggcac ttttctgtta
      841 agattgtagt agaaatcgtc aacaggaaca tagccctcgt catcctcagt aagaacagaa
      901 tcagtaaatg gattatagtt gaaatatcgg tcatactcct ctgcctttat ttttgcgtca
      961 tggtcaccat aaataacaac tacagtgtta tcaagaagtc ctgcattatc aagttctgtc
     1021 atgaactgac cgatagcctg atctgcatag tgaacagact taaaataaga tccaagcttt
     1081 gtaccctcaa ggaacggtgc agatttttcc tcatacatcc cgtcctcttc gttgtacatc
     1141 ttatacttga aatctacctc atagtcgctc acacgctcaa tgtcagtaaa cggcgtatgg
     1201 tttgtgagca taaggaacgc accataccac ttatcatgct ccttgtttat cttctcgatc
     1261 tttggaactg cctgtctgaa gaacgatttg tctgacagac caagaccaat agtttcatca
     1321 atgttaaaat ctttgttata gttaaagaaa tcatcataac caagggaatg atgtagatta
     1381 agtctgttcc agtaagagcc gttgttgccg tgcatactga aagtataata gcccttcttc
     1441 ttaagcattt tctgggtagt tgtgtaatct ctgtcccaat agttgatagc aactgtaccg
     1501 cttgaagctg gcataagtga agaagcaaag gtaaactcgc tatctgagct tgttcccacg
     1561 ctctcttgag cgtagaaatt tgagaaataa agaccctctc tcgcaagctt gttcatatta
     1621 ggtgtaagct cttctccgtt tatatatgta tccatacaga attgctgaaa actttcagcg
     1681 tggataacta taatgttctt acccttgaaa atatttgtgt acttgttact cttttcggaa
     1741 gtttcttcag tctttgactt atcgtcatag aacttattga aaacctcctc gctctcctcg
     1801 taaccaaaca tcatatttat tttcgcatga gtgcaggaaa taacatcgct gaactgatat
     1861 gtataaagac caaaagtgcc aagaacatat tctctgttcc actgcttagc aagtcttgaa
     1921 taatcagtgc cggtaagtgt tgagcaaaat attcctgcaa gacctatgga aacacaaaat
     1981 gttccaagag catatccacg gcgctttctc ttttccttga cctcggtgat ataatctctt
     2041 gtacgtcttt taagaagtat gtacacaact ataatcacag ggatatccca caggaaaata
     2101 agatcctttg gttccatgat gttctcagta acagcgtcca ttactccgcc gagctgtgaa
     2161 gccgttgaaa taagcgacac tgagataaac gacttataat ttgtatagta tatagaattt
     2221 gccgtacaaa gaatagaaaa aattatggtg agtataagca aatatacaaa ttgctttctt
     2281 ggctttttaa acacatatga aattagagca agcaacagta taactgccat atcagccatg
     2341 agcggtttta tctggctgta attaaactta acagtaaatg ctctaagaag aaatccattc
     2401 agaacagatg caaatacaaa tgttagaaac aagatgttat ccttcaggta tccgcctatt
     2461 ccgctgcgga tctttgttat gaccttattc atttatatct ccttttcaat ttactttatt
     2521 cagtcgcaaa gaatattgta taattttttc tgataaaagt caagcatatt aaaacctttt
     2581 cagtgaaata cacaaaactt tcctcaaaca agccaactaa tttaagggca atattccttg
     2641 aacaaagtat tttttactca cat
//
LOCUS       DAWEMR010000262         2660 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_12335_length_2660_cov_9.666027, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000262 DAWEMR010000000
VERSION     DAWEMR010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12335_length_2660_cov_9.666027"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          617 a          618 c          541 g          884 t
ORIGIN      
        1 catggtgtgc cagcacggtt acttcttttt cacctgtgat gacaaaaccg tctttgagtt
       61 ttgccgaagc gtaaaccgtc attgttccgc tctcaagatc tgacgcactt acctttgcgg
      121 tgtaaagatc gtctttttcg gtaaacgcca attccgtacc gtcgcctgca aatctgaagc
      181 ctgctgtcac gtcagtacag ccctcaggca gagcaaatgt aaattcataa tcacctaaag
      241 gacaggtctc gtcagttccg tttatggata ttttcaaaat ggttgatttt ttcgtttcat
      301 atccgcaaat tgtacattcc tgccaatatt cgccgtcttc cgtattccac ttgtaggcgt
      361 gaagttcctt ttggctgtca gttccgcact cactgcactg cttccaatgt ccgttttcgt
      421 cggatatata atcgccgaag ctgtgttcga taggctcgcc ctcagtagcc tgacagcgtt
      481 tgcaggtctt tggagctgtg caggtcgctt ctttccaatc atgtcccaat gggtttatag
      541 gctcatcttt gtactgtgaa cattcggagc aattaaacct cttcacaccc ttatcgatgc
      601 aagtcggctc ggagattatc gttccctcgt caaatatatg tccgtctact gcgcagtttc
      661 ttattactgt aagcgttgct ttttccgata cccaagtacc cacatagtcc gcattttcag
      721 cgttcttgac cgtacatctg tacatatatc cgttaaagct tatgttcgtt gcacaggtgg
      781 tgaatgtagt cgaccattct tccttgccgt ccacgacttt tgaatcaaga gagaacgttc
      841 cgtcagcttc ggtaacgtct gcaaaagtct ttccatcgtc cttgctcacc tgccacatca
      901 cctcagctcc gtaaggattt ccctgagcga taggaagtgt gaacgtagcc ttttctccgc
      961 tgtttatggt cttattgccg agtgaggctg taaactcagg agaataatat attgtaagct
     1021 ttgccgcatt tgaaactaca gagccgtatt catttgatac aacacagcgc acaagctttc
     1081 cgttgttttc ttttcgcact tttaatgaat gtgaattgtc gtttgtatat gggatatcca
     1141 cgaaagtttc tccgttatcg ctgctttcct gccactgata ttggagcggt tcactgcctg
     1201 ttgcttctac attgaagaaa tagtaacgtt caattacgat agttatttct ccgggcttaa
     1261 caggtttagg ctggctagga gcgtttgttg caccgctttg cgggtgtttt actatgaccg
     1321 gtgctttttc ggagaagttt atcgtaacag ggccgaaaac ggtcggctca gttttttgtc
     1381 cgtcgcctgt tgatacccat gttttgcatc ttatctgcca gccgttcatt tggctttcta
     1441 tagtaggctc gtaaacagaa gaagtttctc cgtcaatggc ggtgaaagtc tttccgccgt
     1501 ccttgctcac ttcccataga tatttgagcc ttactgcgcc atttggcgta ttgtttgtga
     1561 cctcgaattt tgccttttta tccaagtgga atttgtcagg agcagtaaat accggctcgt
     1621 caatgaagat atatgcgtca taggtatatg aatgattgag cttggaatcc cagcctaaat
     1681 aatatacatc tgcctgataa tgcgtaccat tgtcactgtt gcccatacgc cccacataca
     1741 tgcttgtgcc atgggcattt atttttgtgc gcttgtcgtt ttcaacactg taccattcta
     1801 ctcggatatc actgtcttgc agtgaggacg gctcgttttc cattgttttc agcgtaacga
     1861 cgtcgttctt tttcacaaca aagtatttta atgttatagg atcaaaaggt atcctatgct
     1921 ctaagatatt cgttccataa cgcagattaa aataggtgtt ctcatcttca agagtgccgt
     1981 cagcttttat tttaaaagca ttcacgccat aaatggaatc ctgtgacttg agccagagat
     2041 gaagcttgtt tccgtcatgg aatatttctg agcggttata gccataaaaa tcgtcatatg
     2101 gcggctgacg tgtgttagca ctgcctataa actcattgcc atctgcaccc cttgcaacat
     2161 aggtgtactt gtgtaccgag tttccttttg cgtccacagc cttgccgttg tcattgaagt
     2221 cagcgcttcc gcctgatatc tgcaagatta cgttgtcacc aagcgttcca gagtagtttc
     2281 cgctatcaat gatgaccttg ccctctccta caatgttaag tcctcgcacg atcgcactgt
     2341 ttgtaaattc tatcgtgccg cctttcagct cgataggata agtgaaatta tatgttttat
     2401 caggggaggt ctttaatttt aaagttgcgc cgtcatagac aatgatcttc ggaagctcaa
     2461 aaccgctttt gatatctatg ttcattttga tatcgcctga aagtatgttt acatcaaatg
     2521 gaacgttggt gtccagatcg atttcatata tatcttttag atctattccg cttgctaaat
     2581 aaaatgtttc ataatcatgc atatccttga aatatgacat tttaccacgg ccattatcat
     2641 cttttatata gatgtcatcc
//
LOCUS       DAWEMR010000263         2632 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_12457_length_2632_cov_5.317423, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000263 DAWEMR010000000
VERSION     DAWEMR010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12457_length_2632_cov_5.317423"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          953 a          422 c          592 g          665 t
ORIGIN      
        1 tagaatctgt tataaatgac tgtaaggtca caatctactt ttctaaaaac agaaataacc
       61 atgttgaaag gaatattctc gaaaatcttt tggatacatt tgagaacaga atgaaaactc
      121 agattggatg ctgaatttat ttcaatttat tttttggatt tcgctggata ttataaaaat
      181 tatgtagtat tgttggtttg caaaaatcgt atgaaagggt gatgaaatga aaagagtata
      241 ttgcttgtac agagtatcaa ccaaaggtca ggttgataaa gatgatattc caatgcagaa
      301 aacctcgtgc agagaattcg cagagcgcaa tggctggaca attctgaaag agtttcagga
      361 aaaaggcgtg tcaggcttta aggtttcggc aagtgatcga gatgctattc aggatttgaa
      421 agccgccgca gaaaagaaag aattcgatgt gctccttgtc tttatgtttg acagaatagg
      481 cagaattgat gacgaaaccc cgtttgtcgt cgaatggttt attaagcacg gcatagaggt
      541 atggagtgtc aacgagggcg aacagcgtat ggataatcat gtggacaaac ttatgaatta
      601 catacgattt tggcaagcca acggtgagag tcagaaaacg tcagcacgtg ttaaaactcg
      661 ccttaaccaa atgacgttag acggcaaatt taccggaggt gttgcaccat tcggctacaa
      721 acttatcaaa agcggtgaaa tcaataaaat gggaaaagag cttatggaca tagtcgttga
      781 cgatgatgaa gctccgatag tcaagaagat atttgagatg acagttaaag agggttacgg
      841 ctcatatcga atggctgatt atctgaacag tcacggaatc agaacccata ataacagcaa
      901 gtttcaatgt aatacggtta atcgtattct aaagaacagg ctgtactgcg gttatcttgt
      961 gtcaggcggt gttaaatcac cgtatatcga aagactgcat atcattgatg aaaatatctt
     1021 tgaccaagca cagtttatac tgaaacaacg ctcatcaaag aatgaggaaa agcaacagat
     1081 agcaagaaca acaaaaggca gtacattgct gtcgggaaac atatactgtg cccactgtgg
     1141 tcagaagatg gtatcaacaa gctatatcga ccgatacgac agggcagacg gcagtcagta
     1201 cagagttcgc agacagaggt atatatgtac caataaagcg atgaaaagag gtgcatgtga
     1261 cggacagtcg gcttatgtag cacacagaat tgacggtgct gtgcttgcaa cgctgaggga
     1321 ttatctcgcc aaaataaaat caacaccaaa agatatagca ctggaaaaac gctacaaatc
     1381 cgaaatatcg gagcatagga tgaagcagac aaagcttgag aaagaaattg aaaagctgaa
     1441 aaggcaggta gttgaattat ctgccgaaat aggacgatct ttacttggcg aaagtcgctt
     1501 cactcccgat atactgtcca cttctattga caatacaaac gatttgctcc ataaaaaaga
     1561 aattgaatta agtgatataa agtacaagct tgccaatcag caaaatgcga tgggcaggct
     1621 tgatttttat tattcgcaat tcagaacttg ggcagacgaa tttgacaatt caacaatgga
     1681 gcagaagaaa atgatagcct gtcagcttat caaagaggtt aaagtcgcac gaggttatga
     1741 attagagatt atattcgacc ttaattatga acaattctta tctctataaa gcctaataga
     1801 ttgaatttac aaatttattc tgctataata ttttcagctt agaagtgggt gaagatatat
     1861 gagcgaagaa aataagatac aaatttttga agataaaaag attcgtactg cgtggaatga
     1921 gtcagaagaa gaatggtatt tttccatagt tgatgtaatt tcagttctga ctgacagtaa
     1981 cgaacctcgt cggtactgga gcgatcttaa gcgaaagctt aaagctgaag gagcaaacca
     2041 gttgtacgat aatatcgtac aactgaaaat gcaatctcct aaggatggta aaaattacaa
     2101 aacagatgtt gcaaatacag agcaactttt gagaataatt caatctattc cgtcaccaaa
     2161 agcagaacct tttaaaatgt ggcttgcaga agttggcaga gaaagaatag atgaaactat
     2221 tgatcccgaa cttaccatag agagagccct tgaaacatat ctgaaaaaag ggtatagcag
     2281 ggagtggata aatcaacgac ttcaagctat acaggtaaga aaagagctta ctgatgaatg
     2341 ggattctcgt ggtgtaaagc agggtgtaga atatgcaatt ctgactgatg aaattacaaa
     2401 agcgtggtcg ggaatgacaa cccgccagta taaaaatctc aaaggactta aaaaggaaaa
     2461 tttgagagat aatatgagta cgcttgaatt ggttctaaat atgttggcag aagctactac
     2521 aaccgagatt tccaaagaga agaatcctca gtctttttct gaaaacagaa gtgttgcaaa
     2581 agagggcgga gaagtcgccg gaaatgcacg aaaagatatc gaggaaagaa ca
//
LOCUS       DAWEMR010000264         2586 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_12711_length_2586_cov_11.800474,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000264 DAWEMR010000000
VERSION     DAWEMR010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12711_length_2586_cov_11.800474"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          706 a          599 c          502 g          779 t
ORIGIN      
        1 ataacatcaa gctccgtaaa tagcaatagt ttttcacatt tatttaaaat tatatttact
       61 taaaatttat atattctgaa atatatccaa cagtgacgag ccgatataga tatgagggta
      121 tttttcaaaa tcctctttag tggtagagcc agttgttact cctgcaaaat ccacacctga
      181 attgagtgca gtttcggcgt cgatgtagct gtcgcctata taaagcacgt ccgatttatc
      241 cgcaccgaaa cgctctacta tcaggtcaag tccctgcggg tcaggctttg cctttgtgac
      301 atcctctccg ccaactatga tatctacagg caaagagcct gcctgctcat tgaaagtatt
      361 gactatccta tagcgatatt tcgtggaaac tatgccgact tttacccctg cgttctgcaa
      421 ggtctgtaag agtgcgatag tatcgtcata aaaatatgtt ttcttcacca ttacttcgtc
      481 tgcctttttt acataagcct tgcgcatatc ctcacgctga ggattgtcag gcatatctgt
      541 cagcaggtcg aaagcgtcaa caagggtcat gcctatggtg ttgtagatag tcctgtcgtc
      601 ctttggttga taaccaaact ctttcaaaac gtgcttaaag cactccagaa taccctcgga
      661 agagtctgca agagtaaggt caaaatcgaa aacaaaatat ctatatttgc tcataataaa
      721 ctccttttca aacagcaagg gcaacccttt gcggattgcc ctgtatttta ttcaactgta
      781 actgattttg caagatttct cggcttatca acgtcattgc ctctgagaac tgaggtatag
      841 taagcgataa gctgcaaagg aaccactgtc agcataggca tgagcagatc gtctattgtc
      901 ggcagaccga tcttataatc tgccacctca tcgtcaacgt tgatatatcc gtgagtaaca
      961 aggacaacgt atgctcctct tgccttgacc tccttgacgt tgcttatggt cttgtcaaga
     1021 agctctcttt gtgttgcaac tgcgataaca ggcatattgt ttgttaccag tgagatagtg
     1081 ccatgcttaa gctcgcctgc ggcatatgac tcagaatgga tataggatat ctctttaagc
     1141 ttcagtgagc cttccataga aagcgcatag tcaagacctc tgccgatata aagaagattt
     1201 tccttgttta caagctgtga agcaacatac tgacaattat caggtgtttt aaggacaact
     1261 tcaagcatat taggaacgtt ctgaagctct gctgtaagtc tcatgcactc ctcttttgag
     1321 atagctccgt gagcgtatgc tatagcaaag ctcagcagat agaatacaga aagctgaacg
     1381 atatatgcct ttgtggaagc tacagatatc tcagggcctg catgggtgta aagcaccata
     1441 tcagactctc ttgcgataga cgagcctttt acgtttacga tagaaagtgt ttcagcaccc
     1501 ctctccttag ccattctaag ggctgcgaga gtgtcggctg tttctcctga ctgcgaaaca
     1561 agaataacaa gatctccctt tgcaatgata ggatctctgt atctgaactc agaagccata
     1621 tccactgtaa caggtattct gccaatcttt tcaagcacat acttgcccac aagacctgcg
     1681 tgcatagcag taccgcaggc aacaacgaag atgttgttat agcccttgag tctgtcatat
     1741 gtaagaccac actcttcaag gtttggcata ccgtcaacta ttcttggtgt gatagttgtt
     1801 tttatagctg ttggctgttc gtgaatctct ttgagcataa agtgagcata tccgcccttc
     1861 tcagcggctt ccatatcaaa gtcagcaacg agcatctctt tctcaactgg cattccatga
     1921 acgtcaaaga actccacctt gccatcgtca aggacagcta tctcgttctc gtcaagaaga
     1981 tagtaattct tcgtatattt cagtattgca ggcacatcag aagctataaa agcttcgtcc
     2041 tcgccaactg caacgataag aggagaatcc tttcttactg catatattct tcttggaaaa
     2101 tccttgaata tgataccgag agcatatgaa ccctctatct cagcaagaac cttctgtatt
     2161 gtcgccatag ggtcgccctc ataataataa tcaagaagct ttgcaactgt ttctgtatct
     2221 gtctgagatt caaagacata tccctttttc agcagaaaat ccttgatagt tctgtagttt
     2281 tcaatgatac cattatgaac gatagaaata ttcgcatttc cgtgagggtg tgaatttata
     2341 tcagacggct cgccgtgtgt agcccagcgt gtgtgaccta taccgcaagt accgttgagc
     2401 caaccctctc ttttaagctt ttcttcaagg tcagcaagtc tgccctttgt cttttttgtc
     2461 ttgatagcat cgttctcaaa aacagctatt cctgctgagt cataacctct gtattcaagc
     2521 tttgacagtg agtcaagcag cacctttgta caatcctttt ttcccacata gcctactatt
     2581 ccgcac
//
LOCUS       DAWEMR010000265         2569 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_12800_length_2569_cov_8.571599, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000265 DAWEMR010000000
VERSION     DAWEMR010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12800_length_2569_cov_8.571599"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          798 a          470 c          578 g          723 t
ORIGIN      
        1 gagagaaata tgcagaatat agtgactttt ggtgttatat gtgttaaaat aatgtatgca
       61 tagataaaaa agtctattgc aaaaaaatgt ggtgagtcca atgaagatgt gaatattcat
      121 tatgcacaat tatccgttgc aataattgtg ttatatgatg aaattgaatt tatttgcaac
      181 aaaacttgaa gaaaatcaca cctatattat aaaaaatatc gttaagagaa tcgcagctca
      241 ggatgattat gttagggtca actaccaaat tgacaaaatt tccaatgcta catccgtttc
      301 atatagaaca acggttggag gtacatatat tgcaagtgga gaaatatcaa gtaaaggaaa
      361 ccatactacg aaacacatta atgcgacata tattgataaa ggtgaacgta ttttgtgcac
      421 catgtcactc aatccagctc cgtcaggtgt aggtagtcag gcttcaggat atgttagcgg
      481 taaataaaat ttaaactaag gcaatatcac taactatctt tttatgtgat attgccttaa
      541 attatacgat caatttaaca gcttgatttc tatactacct cgcagcaata acccaaaaga
      601 aaggaatgac atatgaaaaa tatcagaaaa acaaaagtat tattgcccat gatgttggcg
      661 gctgtggcgc tgtcagcttg tgaaaatact cccgacaata tcagaagcaa caagcattcg
      721 gctgaggtgg acagcggcga ggcttatgtt tcgtcagaga atatttatga cagctttgat
      781 gctgcatttg aaaaagagta tacaaaattc aagctacccg aaaaatcggc ggtgagcatt
      841 tgcaaacctg acggcgttta tgacttggag cttgcttata tcaatgccga aaatgatatg
      901 gactggctgt cgtcaaaagt ggaagagctg caaagtgcct tggatatcaa gatcgatggt
      961 aagatcgaaa ctgtggaaga tcaggcacag ctccaggacg aacataacaa acttaaaata
     1021 acacgctttg cacagcctta ttgctgttgg gaggcaagcg aaaacggcgt aaataccgca
     1081 gacgctgacg ccgataatat ggagcttttt tatctcgaca gaccaacttc tgaaaatgct
     1141 gacagcggac ttttgtctgc tgcggacaag gcaaagactt tggcggacac gttcagcaat
     1201 gtgcttggag aggagcttga caatgtggtc agtgacgggt atataatcac caccgacagc
     1261 ggtgttggct atgagatcga gatacaaaag gcatataaag gtatcggcat acaaaatgtt
     1321 ttttccacga atgctgacga tacgctgttc gtgaagggca atgcgcttat gacctattca
     1381 atgcagactt ataccgatct cgacgcaaac tttggccccg aatattacat aggttgtaac
     1441 gctttcaagg tggcaaaggc tgagaaaata gacaaaatgc tgtctttcaa gggcgcttgc
     1501 gatctgcttg aggaaaatct tgcggacaag atcgatatac agtttgatga cgtcaagctt
     1561 atgtatgaac ctcgtggcga gagtaccgaa gtcggcgaaa gtgcggataa tatatgctgt
     1621 acgccaaagt ggtattttat tatcgacgat aaacagccga caggtctgca cgcaataaat
     1681 tatgtgacag tcgattgcgt tacgggcgag atatatgtgc ttattccata gggggtgggg
     1741 caatgaaagc acttaagtgc gaccttaaca agagtatgct gaatatcgga tatgtcggtg
     1801 cggtgctgct cacgaccctg ctttgcttta ccgcaaaggc gtataccgac ccgaacacgg
     1861 cgaagatata cacggtgttt gagtgcattg caaagcttga caaacagctt tttgctgatg
     1921 attattcctt cagttcctat atgatattcg caaaaggtat gtcgggatat atggcaatgt
     1981 ttctgcctat aatagcggct tttcccttta tgacggcatt ttgtgcagag cgaaacggcg
     2041 gctcaatgcg gttcaacata acccgaacag gcagtttgag atattatctg tcaaaattct
     2101 gctcagcatt tctgtgcggc ggttcagccg ttctattcgg atatataatt ttcggcgtta
     2161 tcgtgcggat aatatttccg ccattgagcc aatacgaact ttccgcagat gatttatcat
     2221 tcgtgcttgt gaacaataaa gacgagctta cgggcgtgtt gagatatatg ctttcggcgt
     2281 ttttgtatgg agctatgagc acaatgcctg cactcttcat aagctcattc tgcaaaaatc
     2341 cgtatcttat aacctgcgtg ccatttatgg taacatatat gtggcagata actgtgcaga
     2401 aacttattgc aaattcgatg atgaaagagg atttcgacag ggcaagtata ttggagaatt
     2461 tttatcccaa cagcataatg aatttgtcat attacacagc ttttgctgac acaaaataca
     2521 ttctgttttt caatttcggg ataatacttt tatctgtgat aggttttat
//
LOCUS       DAWEMR010000266         2549 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_12905_length_2549_cov_7.541700, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000266 DAWEMR010000000
VERSION     DAWEMR010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12905_length_2549_cov_7.541700"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    256..265
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          660 a          593 c          463 g          823 t
ORIGIN      
        1 atccagatat acacaccgct tttgtcaatg attcataata gcagatgata gttttatgct
       61 tatccccaaa aaacatttac tcttcccttt ctcattatcg ttccattttg aaccattata
      121 acatattctt cattatatgt caagaaaaaa tcagcgaaga agcaataatg gtttattagg
      181 tatcgcaaat ttattaaagt gaaaaaaatg ctctgcggga tcacactctc cgcagagcat
      241 tttgttttag tatagnnnnn nnnnntaacc accgtcagcc tcagaatatt taaggcacat
      301 aaactgccac ttgtaattcc attgtgggct gtcatagacc ttttctccga cttccagcag
      361 ataagtcact ccgtctatct ttatctcgcc ccagaagtgc tgtcctccgc cattgcggtt
      421 gcccataaca aggtttgcgt catagccgag ataaaccatc atttccacca gtgcgccatt
      481 gtaatcagaa cattgacctt ctttgtaaac aaagatattc ttgctatgcg agcctgtcgg
      541 tatcctgcca tatttcaaat tctttcttat ccagtccgca gtatattcga ccttctttgc
      601 cgccgaccaa tctttcttga aatgctttga ggcaaagttt ttaagtatct tcttctcctc
      661 tgctgtaaga gtgtgcgtcg aggtctttgt tttctttccc tgaacgttgt agatcttata
      721 agtgctgtgg ctcttttttg ttgccgcatt aagtcttgaa acagtgttta tcttgcattg
      781 gatacccgtc cagctactgt aaaccacctt tccgtttatg gtcttataag ccctcatttt
      841 gaagtgataa ttctttgaac ctgaaaggtt tgattttgta taagaggttg tgctgttctt
      901 tgttatcttc ttgagctgaa cataatcgtt atagcaatcc tttggcatac tatagtaatc
      961 gttatgtggg atagtccact catcgccctt gcaccagtat atctgatatc catcagcctt
     1021 tgtgttcttc ttccagctta ttgtgacttt cttattataa gcctttaccg tcattttcgg
     1081 agcagataac tttgtgttgc cttttaccgt tatataaggg ccggtcttag agttgtattc
     1141 aagctttacg gcataagtat aactcgttgc ctctgaaagc ttcttgtcag tatagcttgt
     1201 ggaactcgtg accgtcagac gcttgtaggt ctttttcttt gaatcatagc gataaaccct
     1261 gtatttgcaa ttgccgatct tgttccagct aagctttaca gaagtggtag agacagcggt
     1321 agccttaaat cccgttggct ttccaaggac gatcttgaat gttgtcgaaa cagggtacct
     1381 gctttcgtag tttccgccaa gagtaacaac agcggtcgct gtgcctacat ttgtgttatt
     1441 gtaatatgtt acagtatagt cagaactctt gatcatattt ccgtccgcct tgacaaaaag
     1501 gctcggtttg acagctttgc cagtataatc cacatctgct atctttagta tctcgacctc
     1561 agaaacagct ttcgggctga tagtgaaagg cactgtcaca tatcctgtat agccgttcat
     1621 tcctgctatg gtcatatagc ctgttccggc attcacgttg tttgtatatg atacattata
     1681 atcaacgcca cgtcttagca cctcaccact aactgtaaca acaggtgcag gagtctgagc
     1741 gtagcccgtg taggtgcagc tagtatccac agaaatattc tcgctggata tttcagcctc
     1801 tacaacgcta tactcaaagg aatcggttcc attaaagttg ccgatacctt taatataagc
     1861 ataatgccag ccaagctcgc cacagtcgtc ctcatattcg atctcatagt caacgccctc
     1921 agcaagcaca atatcattgt atgtaacaat aggttttggc tgaacaggtt taccggcaca
     1981 gtagctctgc tcatactcgc agtcaatatc cgcacggtaa atatcagttg gcacgatatt
     2041 gaaattcttt gtaaagcttc ctgtgtaact acctatacca gtgaaagtag ccgtcgctat
     2101 acctacgttt atgttgttct cataagtaac aacatagtcc tcgtcctcag tgagccggta
     2161 atagttgttg ctgtccataa gagaatagcc atagagcgtc ttttccgtgc cgtcatacgt
     2221 ctgatcgcct tcataccaac tgctatacat atcctcagtc aactgaatgg cttcatcgtt
     2281 ctccgacgta taaagaccga cttcctctcc gccagcagga atttgctcct ctgcctcagc
     2341 accaaagcat accactgcac tttgcagtgc aagacacact gccactgccg tcgtaagcgt
     2401 ccgcttaaat aacttcatat tatttttttc ctttccacta taatttctca catattacta
     2461 attttatcac aaagatgtga caatgtcaag ataaaataca acaaatagaa agcctcatga
     2521 cattcaatta gatcgtgaag tggaggatg
//
LOCUS       DAWEMR010000267         2505 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_13163_length_2505_cov_5.673061, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000267 DAWEMR010000000
VERSION     DAWEMR010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13163_length_2505_cov_5.673061"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          720 a          554 c          367 g          864 t
ORIGIN      
        1 ttccctcaaa tattttgaaa aatcgggatc aaccatgtgt ttgtactttc tactcattgt
       61 aagtctaaaa ccttgcttaa aaataacttc gtcatttttc atatccaaat taaaaacatg
      121 actaagatta acaagatact ttttatgaac tctcggaaaa tcgtattccg caagctcact
      181 ttcaaggtca ccgataactg cacgtacttt agtatgaatc tgtgaaccat actcatccaa
      241 gatacaatat ttcacatgat gattgtcact ttcaatatac aaaatgtctc tgtattgaat
      301 gcagattcgc ccatgctctt catcgctcaa aattattttt tggtgctgct ttaaataaac
      361 agaaagcttg tttaaaacat taacaacatc tttatttaat cttgaaacgc tagacttttg
      421 tataaagtaa aacggttgaa attccatgct tccgtacacc agttcattgt gatttgtaac
      481 aaatattata taacaatccc ttctttgttt tcttaatatt tctgcaacat caaagcctgt
      541 gaattcaggc atatcaatat ccaaaaaaat cacatcaaaa aatatgtgct tgtgttgatt
      601 caataaacct tgaccgttat tgaaaccttg aacatttaca tctttaaaaa gctctcatta
      661 tcatcacaaa ctgcgatttt aagcacatcc ttcaccacct ttgtatgaca ttatatcaaa
      721 catttttcac taaatcaata caccttttat taattatgtt ataaaatcac ttacatccgt
      781 atataaacgc ttggctttgc aataattgtc accaaacaat ccgccattca aacatctata
      841 tattccggaa tcaatatgat tattcatagt tctgagcctg ctcttccttg atacccagcg
      901 acagcttttt ttgacttatc attctccgca gtctgctgtc aactgtccgt cttaccaact
      961 tatacttctg cgtatagtca tcttcgatgc acctactgag atttgcaaac aaaccaaaga
     1021 tcgtattggc aagcacagcg ttttcatacc gctccatatt ctcaagcaga tcttgagcat
     1081 actcgtttcc gtcatttgca gactttgtga accattcgac agctttttcc ttatctcgtt
     1141 caatgtcctt agccccgaaa agatagatcc tgccaagctg ataacttgcc caatgatttt
     1201 tggcagcaca attctcaaaa taattgacag ctttttgtat atcatacttt tcttcctgca
     1261 aatacagctt tgcaagtgca tactgcgtat actcgctatc ttctgccaag agcaaatact
     1321 tttctgcttt gtccagattt tgaggcatta tctcgccctg caaataaatt tttcctagcc
     1381 tgtaacaagc aatgacatct ccgctgtccg cacactcagt aagcaaggct attccctttt
     1441 caatgtcctg ttccaaatgc tctcctgaaa tatattcaag ggcaaggaat ctttttgcat
     1501 ttctgttttt tagtttgaca gctttctcaa gcaattttac tgcactatcc gtatcttgcg
     1561 gaatgtgttc acctatcaaa agtgctttgc catactcaaa caagccgttc ttgttattca
     1621 tttcagctga acgtctgaag tattcgatcg cttttgtaac gtctgagtct gttccaagtc
     1681 caagcttgaa catctgtccc aatttgtaaa acagatcgtc gttcgccata tcatcgcttt
     1741 cgattttcaa aaatcccgac aaagcttgtt tgtaatatct ctgtgctgta tcgttatctt
     1801 tggttacata ctctccatat ctgtacattt gagcaataga ataagccgca taaggctgcc
     1861 cctgagagga tgatctctga taccaaagaa aagcctgcga cagatctttc tctataccgc
     1921 tgccatagta atacagattt gcaaggctaa actgagcaaa cttgtttttc tgttttgccg
     1981 atctttcaaa ccactcaaag gctttttgat agttctgctc cgtaccaaga ccataacaaa
     2041 acattttgcc tatacgatac tgaacatatg gctttaattt ctttgagttt ggttctattt
     2101 gcaaaaagcc ctgcaaagcc tgtgtgtatt ttgcaataga tatttcttcg cttttttcgc
     2161 caagctagtc ggtagaataa agctttccga gatcgtaaac tgccaaaaca tttccacgct
     2221 gtgattcaga taacaaaagc tgttctgctt ccttaaaatc attgagctta gcgtctttgc
     2281 catagataag cttacaggct tttttgtatt gatcgttcca tttgatatag tattttattg
     2341 ccgcagccga ttcgtctgtt tctgtctctg ccaacggttc tgcttcatca aacagaggtg
     2401 acatattctg aggtgtatta tcaaagtatt catcatttgg ttcagatcct ttaatttcag
     2461 tatttacatc aaagggtttc atattaagta ctgtacgaat tatca
//
LOCUS       DAWEMR010000268         2475 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_13343_length_2475_cov_10.628099,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000268 DAWEMR010000000
VERSION     DAWEMR010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13343_length_2475_cov_10.628099"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          811 a          482 c          535 g          647 t
ORIGIN      
        1 gagttactgc tcccattgca gaaaatgcaa ttaaaagatt ctaaagatgt gggaaaatta
       61 tttgcaccgc tttatcgtcc agaatttccg catctgtttg gtattgatct tttttacaga
      121 cggcaaaatt ctttaagaga ggtcagcaga gatgacggta tagatatcac aagtgatgac
      181 actgctgaaa atgtggcaga tatcagaaac gaaagatatg ttgagataat aagaggactg
      241 ctgtcttttg ccgaaaacag ggcggtcttt aattttagcg aatatcttga cactgtatct
      301 aaaacgcagc tgcatgaaca aatgcaggaa aaagcattgc ctgacgtcat gctgaagctc
      361 tatggacttg gagaaataga tatcgcaggc tggcgttcgg cacagcatga cataatcgtt
      421 cccgtgggag aatttgatct ctcttattgt ctgagcgagc ttcctgaaga atcggtacaa
      481 atggagtcga taatgatata taaactcgat gatgttataa ctttatctga tcataaaggc
      541 ggacatatcg agatgaatga ttttaagatc gaggtgagaa gatgaggcct gaaagtctga
      601 aactatttgc aaagcttatg gaaaatggtt acattacccg tgagcagtcc cctgcgtatt
      661 atgaatatta taatgatcct gaaattgctt ctgaacttgc gatcatggaa caggagcttg
      721 atttcactct ttacaaaacg aacggcagac tttatctttt gccagatcca aacaacgaat
      781 tattttcgca ggataacaat gatttccgcc gcagcgttgg caatgaaaag cttgaagagc
      841 tttatttgct gaattatatg gcgatctttc ttttacatga gctttacggc ggaaaaggaa
      901 acacactgca aacaagatat tacatcaaag aatcagattt tctttccgag ttcacaaagc
      961 attgtgagca ggtacgctct gaaggagaat ccttaaaaaa tgcttcagca cagtacagca
     1021 tagattttct gcaccttgct gacaactggc tcgcaaagcg tggtgatgaa tccaactcta
     1081 tagataccaa acatggctgt ttcatgaaga tcatcagaaa gtttcgtgat gaggaattgc
     1141 tgtatgacgc tgacggtgta tggaaaccaa cggtcaagct gaatgatctt atgccgtatt
     1201 atttatctca aacccgcatt actgaaatac actctattct tgaaggagga aaaccagatg
     1261 ccagcgatcc gtaagatacg aattgtaaat ttcaggttca acaatggtgc aaagctgata
     1321 ccagacgaga ttttctgcac tgagaacgct gaaggaaaac caatagatac tctttttaat
     1381 ctggataacg gcggaggcaa gtctgttatc gttcagttgt tgctccagcc tatttgcccg
     1441 aaggcaaaag ttcagaatcg taatatcagc gattattttc agaaaggcac ggatcatgcg
     1501 tttgtcttga ttgagtgggc attggacgga tcacataata gtctgctcac agggatcgca
     1561 ctggctgcaa gcaccacagc agatgacgaa aatgagagca agaccattcg ttattatact
     1621 ttcatacatg attacacaag agccggcgac aagctcgatc ttatcagtct ccctctttca
     1681 cagcgcacag gcagtcatat ccgcccaatc tcttttgacg agcttcgcaa atatctgcaa
     1741 aacagaaggg tcgaatacta tccttctgat tcattgcgcc gttatcagaa gcgtttagag
     1801 gaattcggta ttttccatac cgaatgggaa aacattcttg tgccgatcaa tgcattagaa
     1861 ggcggcatta caaagttctt tgaggaatat aaaacgagcg acagattact tgacaaattt
     1921 attattcctg gcgttatgcc gagcaacgat acgtcaacaa gatcccttga agaaatgttt
     1981 atcaactatg cggtcagcta tgccgggcag gagaaaaatc tactcttaaa gtcgcagatc
     2041 agggatttta cagataagct gcagggtatg cttcagctaa taaaggatat gtggaacgca
     2101 gaagatcaga agatacaatc tatacaccaa ttggctgatc attcatttac acttcagcat
     2161 caaatcaagc tgaacaaaga aagacaacaa cagcttgaaa aagcaatatc cgacttaaag
     2221 acaaaacaga agcacatcaa aaccgaagca gcatcaaaag ctgtttatct ttcacaggct
     2281 gattttgata aatcaaccat atggcttgca gaatgtaaac agaaaaaaga tgatgccgaa
     2341 agtcagtatg acaggcttga acataaagtc aaggccctga acgctgccaa agagtacaag
     2401 gagcttctta aacagcaaaa tgatgagaag gcattgactg cacagatcag agagcttgac
     2461 caaggaacgc aggat
//
LOCUS       DAWEMR010000269         2467 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_13391_length_2467_cov_12.818823,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000269 DAWEMR010000000
VERSION     DAWEMR010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13391_length_2467_cov_12.818823"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          756 a          428 c          650 g          633 t
ORIGIN      
        1 gttgtgacta actcgttctt ttttatgata gcacaggacg tgggaaatgt caatagaggt
       61 ggggcggata ggggcgttgg ggcagaaaaa taccgataca ggggagctgt atcggtcagc
      121 ctgtcgcaaa agcctacctt ttaataaaaa aaaggtaggc ttttttcgtg aaatgtgata
      181 taatatagta gggtgataaa ggttttgtta catcttgtca acataaaact ccctgtcggt
      241 ttcaaagcag tatgcgcaca gccgtccacc tgaggcacaa ccacagtcta tggctatgtg
      301 tccgttggct ttgtaggctt cgggactgcc gtggtttggg atattggcgg taggggtgtg
      361 acctgtcaca aggaaagaat tcgggtcgct gaaatagcgt tttgaatagt cagcacgctc
      421 ccaaataagc tggtggaggg tatattcatc aaggggctta tcctcgctaa agcccccaag
      481 acctgcatgg gtgaggacaa aacgtttgcc gtttatggtg atatcctcat acattgagaa
      541 gcttttaaga aactcgataa aatccatgcg gtcggctttg gagagggaga taaattcgct
      601 gaaaggtaac cgtcaaatcc tccgcaccct cccaaaaata atcagaccct gcaagagatc
      661 accgtgggca agcaaaacgt cgctggaggg taatgttgag ataacggtat tgcaagatga
      721 agaaagagtg cggaaggatc catgctctga cacacttggg tatcttaatg gtttggacga
      781 tcagcaaagg ttggagctag tgaaacatca caaggatatg gtaaaactta cgcctgaggg
      841 tgaggaagaa gaatataaga agcactgcca aagctacgag tttaaaagag caacggattc
      901 tcttactttt gtatgtatac gccgaatggc tgacggagaa tttcaggtat cgcatttgta
      961 ttccgacaat tattattgca gtgcggaaga tatgtttgaa aaaaatcgaa agcaaaatga
     1021 gtgatgattc ggtcattgag tgtgttatac tgacttatag gagaagctat ttgcctgagc
     1081 ggatgatata taatagggac gcaaattttt atgcgggctt ttattacaaa gacggcgtgc
     1141 ttacagaaaa gagtgaggaa gacgctttta agctttttca tttactttac tcagaaaagg
     1201 tcgatcagga gtatgaacac aaatatggtc atttagagaa gcgacggata agatgtaaag
     1261 gaggacaaaa tgccgaagca ttggagcaaa ctgcaaagag agatatacaa gcttatcgac
     1321 agcaggatag ggattcagat acattgcagt gtttacagaa tggatagccg ctggggcgga
     1381 actgacctgc ctagatattg gataacatta ggcaaggaga taatttttga ttatcctaaa
     1441 gactttttgg ataagaagct gcctgagcga gggttgataa tatcatgctc acataatgaa
     1501 actgtggagg agctttatcc ttatgtaaca gagataagcc tgatatcgga tatcatcagg
     1561 gagtatatag acacgccgct agcggatctg ctatcccatg attttgaagc cgatgacctt
     1621 gggataacgg atattttgaa agcctctgac aggaggatag gaaagcgcag gcgggcggag
     1681 tattttaagg acactgacaa tgaagcggtg aagaagattt taagagtcag gggagagatt
     1741 tgactttgca gggtgcggcg tgatataatg ataaaaaata tctatctaag gagtaaaaaa
     1801 tgaccgacta tatagaagct cacaagtttt ccagcaacaa caaggaagca ctgcaaaatg
     1861 acaaaaagtg cggctgtttc tactgcctga aaatttttga cccaaaggag ataaactttt
     1921 ggataaaaga taacacagaa acagcagtat gcccatattg ctgtatagac tctgttatcg
     1981 gagaatcaag cggatatcct atcacaacgg aatttttgac gaaaatgcat gaatattggt
     2041 tttgataagc cccattaaaa cggaaaggac aagaaaatga cactgcatta ttttgctgac
     2101 aaaaatgccg acacagttct tatccagcct gtgggcgacc atgatctgcc tgaggtagaa
     2161 aacgaggtgg cagagataaa aaaactcacg agtgtggatt ttcagcttat tgcggtgaag
     2221 gtcgatgatt ggaacgatga gttatcgcct tggaacgctc ctgcggtatt tggcaatgag
     2281 ggcttcggca atggagcgga agacactctg gcggagatat taacgctttg ctcggacaag
     2341 agcaaggatt attacattgg cggatattct ctggcagggc tttttgcctt gtgggcggcg
     2401 tttcaaacgg agaaattttc aggcgttgcg gcggcttctc cgtcggtgtg gtttcctgat
     2461 tttgtgg
//
LOCUS       DAWEMR010000270         2464 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_13404_length_2464_cov_10.726027,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000270 DAWEMR010000000
VERSION     DAWEMR010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13404_length_2464_cov_10.726027"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          742 a          586 c          493 g          643 t
ORIGIN      
        1 ccatgaaaaa atgggctata ctataatagg tacgttccat agatgtgcct ttaagttcaa
       61 caagtgctat catatggttt ggctcgaaaa aatactcacc cagaccgata tcacccctct
      121 gcccgttatc ccattttcca aactcgataa agccttaatt aacaaaatag ttcttgaata
      181 aaggcagaga atatggtaaa ataatatcat tctgtttgtt aaggagcgca aaaatggatc
      241 ttcaggaatt aagaaatgaa ataaacgata tagacagcca aatgcaggag cttttcaata
      301 agcgtatgca gataagcttc aaggtggctg agtataagat agcaaacaat atgcctgttt
      361 ttcagagcaa gcgtgaaaac gagatacttg accatgtagc agaaagctca ccaaagggct
      421 tggaaagtgc gtcaagagtg ttcttccaaa acatcatgga tatttcaaag tgccgtcagt
      481 accaggagtt ttttgcagac tcagataaga tagagtacaa aaagttggac ttgacaggtc
      541 ataaaaaagt cgctgtacct ggtaccacag gctcatattc acatatagcc tgcaagcaga
      601 tattcagcga ctttgagcca atgttctttg aagattttga ggacgttttt tcatcagttg
      661 aaaacggctc agcggatttc ggcatacttc ccatagcaaa ttccactgca ggctcagttg
      721 gtcagaccta cgagcttatg aaaaagcatg acttcaagat atgcgcagca actaaggtaa
      781 agatctccca ctgccttgcc gtaaaaaaag gcacacgctt tgaggatatc aaaatagtct
      841 attcccacga gcaggcgctc agccagtgtt ctgagtttct ttctcagaac ggacttaaaa
      901 gccataaata cgcaaatact gcccttgcgg cgtgctacat aaaagaaagc aatgaaccgt
      961 atgccgctat ctgctcagaa gcctgcgcac agcagaacga tcttgagata ctcaggcata
     1021 atatagccaa cgccgccgag aattacacaa aatttattct catttcaaag gagactctct
     1081 gctcggaaaa tgctgacatc gtgtcagtgt ccttggcact gccacatacc tcatctgcac
     1141 tttacagact tctcaccaaa ttctccgtgg caggtcttaa cctttccatg atagagagcc
     1201 gccctatcgc aaacaccgat tttgacgttg ttttctatct tgattttgag ggagcaataa
     1261 cctctcctga cgtggcaaag ctcatgagcg agcttaattc agagctttca tatttcaaat
     1321 ttttaggtaa ctacgaggaa atataacagt acgattttta ggacaggagg aacaaaaatg
     1381 cgtataggac acggctatga cgtccacaga ctgaccgagg gcagaaagct cattctcggc
     1441 ggcgtggata taccatatga aaaaggactt ttaggacatt ccgatgcaga cgtccttgcc
     1501 cacgccataa tggattccat acttggtgca ttggcactgg gcgatatcgg caagcttttc
     1561 cccgataatg accccgacta tgagggtgca gacagtatga agctcatgga aaaagtcata
     1621 aacgttatga ccgaaaaagg ctggaaaata ggcaatatag actccaccat ctgcgcacag
     1681 aagccgaagc ttgccccata tatcacctcc atgcgggagc gtatagcaga agcctgcggc
     1741 tgcgatattt cacaaatatc agttaaagcc accactgaag aaaaactagg cttcacaggc
     1801 gaggaactag gcatatccgc cacagcggtc tgcctgcttg aaaaaatttg atacaaaacc
     1861 aaaaggactg ctgtcttaaa ccacagcagt cctttttatt gctactttgc cttatgtgca
     1921 actccgtcat tgtcaatgtt atatccgtca acagtagtgt ttatgagcat catgccataa
     1981 tcattgaaat aatatcgctt tccgcctatc ttcttccaac ctgtcacaac agtgccgttc
     2041 ttgttgttgt agaacttctg cccacgccag cttacccagc cggactttag atatccgtcc
     2101 tcaccgaagt tgtaatactt gtcgtcaatg tacacaggac ctctcaacgc aacaccattc
     2161 tgcaggaaat atttctttcc tgcaatagtc gtccattcat tctgcaaaac atgatcgctg
     2221 tcaaaataaa actccgttcc gtctatttct ctccagcctg aaactgcctt gccatcgccg
     2281 tcaaaataat aggtcttgtc accggctgtt acccacttgt ctttctgagc gccgttcttg
     2341 tcaatgtagt aaagccctga atcatcactg aacagcccct ttgaaagctt gccgtcattg
     2401 tcaaaatgat acagcttgtc ttcaatagtg tgccaacctg taagtgcagc ctcgtcctcg
     2461 ccaa
//
LOCUS       DAWEMR010000271         2314 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_14324_length_2314_cov_7.571049, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000271 DAWEMR010000000
VERSION     DAWEMR010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14324_length_2314_cov_7.571049"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          623 a          481 c          492 g          718 t
ORIGIN      
        1 tgatgttttg aaagagaggg ctaacaggct ttatgaattg cgtaagagcc gcaacctgac
       61 gcagacagcg gtagcttata ggctgaacct ttctctttcg aggatatcag cctatgagaa
      121 cggcgagaat attccatgtg atgtgttgag ggtatttgcc aacttttata gtgtatccat
      181 tgattacata ctgcgctttt cggacaggac agttggcagc tctggcgtta atcttcacat
      241 ttctgaacaa aagctgctgg atctgtacag aaatctttcg cctgatggga aagccgttat
      301 cgacggtatg gtaaatggct atgttcctct caaataatga gaataatcgc ctgcaagcat
      361 gaaaatgcta tatacacagc cctttcgcaa attattccct tttgcgataa taagaccgta
      421 ttccgcataa tcaagcggaa ttttttgttt tcctgacgag aaaaatgacg gcaaataatc
      481 ttttaagcca ccgagaacag atccgaaacc gacttcatat actcgacttt ttgctccatt
      541 gatgaatgaa catagcgatt aagcgtgatt tgtacgttgc tgtgtcctaa tatttcagac
      601 agcgacttta cgtcaactcc tattgctatg cagttcgtgg cgaacaggtg acgcagcgaa
      661 tgaaaagata cgtccgacag gtgaagttca gcaaggatcc gcttaaatcg gtactgcatt
      721 actcttggct ctacaattcc ttgcgttccc gaaagaagat aacagctgtt acctgttcta
      781 tgattactca aataaggtat aatgaactcc ggcagcggga ttttccgaat agacgagctg
      841 ctcttgggcg aagtcaccac cagctttgtg gctgtatttc cgtcaatatt cttaattctc
      901 tgaaccgtgt tcctgacggt cagaattctt ttttcaaggt tgatgttttc ccatttcagc
      961 gcacaaagct cacctatacg gataccagtt gccgcagata gtaaaatacc gacatttgat
     1021 gatgtcgggt tagaaataag ataattgtta agccaagtct gctccgtttt atcgagcagc
     1081 tctttttctt ttgccttgcc tttgggagtt gccataaatg ttgatttatc ctcataacca
     1141 aaacgttttc tggcaaagcg gcacgctgat ttgattaccg ctgcaatatc agcggtatat
     1201 ttagcagaaa gtccgcttgc cagctttgaa tgcacaaagt cattaagttt ttcgaccgtc
     1261 agcttttcat aaggtgtttt tccaagcgat gagctgatgt gtttttcata tttcatctgg
     1321 tagttcatat aagtggattc ttttactttt agccgaatgt ctgcaaacca aaacgaaaaa
     1381 agattgttaa ccgtcagatt tttcggaata gacgaaacat cggttttaga acgtttctcc
     1441 gataatttgc ccttgacctc ggcataggtt ttcccgtata ttgaggagta tcttgctgtt
     1501 ccgtcagcct tgactccgct tttatagcgt ccttcccagc gaccgtcttt tcttttgtag
     1561 atattctcac cgcgtctagc cattgtataa cccccggttc tgacaacatt tttgacggta
     1621 aatacggctg taaggtggat ttttcttttc agttctgccc attttacggt tcaaatttgc
     1681 tgtctgtaat agtaattgag tgaattttgg ctttagaatt gcgtttttca gccgatttgc
     1741 cctttcgcaa aagggaataa tttgcgaaac catacactca ttatattaca aataatctag
     1801 gtagtccaac aatggtaatt cgtaattctt gtataacgaa acatcattcc tgataatcgg
     1861 gatttctcag agcggattta tgatattatt aaagtacagg caggaatgct tgtgaaagct
     1921 cattgaaaac tgaatagcaa agcaaacaca taagttgcag tgctgtgctg tttctgacag
     1981 acaagcgcca agacattcct gctttcgatc tgatttggta tctcattatc cgtgatcacc
     2041 tacttttttg cgcgagaata aaagatacca tgtcacctgc cgacggttcg caggtgccct
     2101 aaacgcatag ggtgtcctta aaggaacgag cgcatttgca gcctgcaata taatgatact
     2161 tccggtgacg gctgtcccac aggaatgggc agaggtgaga gtcctatgtg cgaatgcgta
     2221 actactcgca gtttgctttg agaagatata gtcagaaaaa tgggtcggac tgtacgcaga
     2281 aaacgtaatg tccgacctgt tttagattgt agtc
//
LOCUS       DAWEMR010000272         2292 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_14464_length_2292_cov_11.003129,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000272 DAWEMR010000000
VERSION     DAWEMR010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14464_length_2292_cov_11.003129"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          585 a          585 c          475 g          647 t
ORIGIN      
        1 attgcgttgg gacaacattg ggaaagcaat tgcataagcc ccatggcggt gtccttgccc
       61 ttggcatata cgttaaaacg cagtacattc ttcttgtgct tcgtgcagac gattatctcg
      121 ttggtgtagc tgatgaaatt catactcgct gtgtgctggt aaatgccgta ggcttcctct
      181 cgccagcaca tctgaaatgg cgctttcttg ttggcaagca ccctgtcgga tacgataata
      241 taatcgtcct gatattgtat gttggcgtaa agctcgtcag cctgctgtaa aagatcggga
      301 ttggctttga atatgccgct cttttcaggg tgcgtcctcc gtgacgtacc aaatataaga
      361 aatataacac caggcgtaag cgcaaaaagt ccaagtatca atacgctgta ttcaccggct
      421 actaccgccg caaatgagat aaatccaccc attagcgtga gtatcacacc gccaactatc
      481 attgctatca ttctttttcg tatgttggca aatactactt ctcgtcccat ttttgttacc
      541 cctctttact ttttatccgt gattctgctt tgcaacaaga ctctctccgc agtatatctt
      601 cctgagcttc ggcttctcta tcttgcccgt cgggtttctc ggtatgtctg caaaaattat
      661 cttgtgcgga cgcttgtatc tcggaagctt ctgacagaac gccattatct cttcctctgt
      721 gcatgggtgg tctggcttta gctcgattat tgccgccgct atctcgccca aacgctggtc
      781 aggcagacca attactgcca cgtccctgat agcgtcatga cttctcaaaa agtcttctat
      841 ctgtacaggg taaagatttt ctccaccact tataataacg tcctttttgc ggtcaacaag
      901 gtaaataaat ccgtcctcgt cctcctgagc catgtcgccc gtgaaaagcc agccgtcttt
      961 gagagtttca gccgtcgcct ttggatctct gtagtagcag gtcataacgc caggaccttt
     1021 tacgcaaagc tcgccaactt caccacgctt tacagtgttg cccttatcat caactatctt
     1081 gaccttccag ccaaagcctg ccttgcctat tgcgcctacc ttgtcaatgt tatccatgcc
     1141 aaggtgtaca cagccgggac ctatagattc gctaagacca tagttagtgt catatttgtg
     1201 gttagggaaa acctttttcc agcgtgcaat aagtgacggc ggaacaggct gtgcgcctat
     1261 gtgcataagc ctccactgtg aaagctcata ctttgaaagt gtcacctcgc cgctgtcgat
     1321 agcgtcaagg atatcctgcg cccaaggcac aagaagccat acgatagtac acttttccct
     1381 tgatacagta tcaagtataa attcaggctt cacgcccttg agaagcaccg ccttgccccc
     1441 tgagataagg cttccgaacc agtgcatctt cgcacctgta tgataaaggg gaggaataca
     1501 aaggaaaacg tcctcctttg tctgaccgtg atggttctgc tcaactcttg cggcgtgcat
     1561 aaggctctcg tgattgtgca ggatagcctt cgggaagcca gttgtgcctg acgagaagta
     1621 gatagccgca tcgtcctcgt cagtaagctc tatgtacgga gtagtgcttg cacaatttgc
     1681 agtgagcctg tcataatgct ctgcaaatga cggacagttc tcgccaacat agaaaagaag
     1741 tctgttcttg cttatctcat cagctatctc ctcaactctg ccgataaatt caggaccaaa
     1801 tacaagaata tccacctcgg caaggtcaag acagtatttt atctcctctg gagtgtatct
     1861 gaaattcagc ggcactgcaa gcgcacccgt cttgaggata ccaaaataaa taggcagcca
     1921 ttcaagacag ttcataagca ggatacccac cttgtcaccc ttttttaccc cacgggataa
     1981 aagcagattg gcaaagcggt tagccttttc gttgaaaacg ctccaggtta tctcacggcg
     2041 ataatgacag actgggttag gctcgataag ctcatattcc ttccaagtca cacgtctggt
     2101 ttctctgatc tcaggattga cctctaccag agcaacatcg tttccgaact ctcgtgcgtt
     2161 acgctccagt atctcggtta taggcataca aaaacttcct ttcataatta aaagcttaaa
     2221 agctagaaaa cttaaaagct taaaagtatt cacaaacata tatgttatta ttttatcata
     2281 tgatgacaaa aa
//
LOCUS       DAWEMR010000273         2272 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_14619_length_2272_cov_9.886333, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000273 DAWEMR010000000
VERSION     DAWEMR010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14619_length_2272_cov_9.886333"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    545..554
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          725 a          359 c          512 g          666 t
ORIGIN      
        1 aggtatataa tatgttgtta tctgtaaata atattttaaa caactataat tcatttacat
       61 tcgatctgga gaaaataggc aaggagatga tgatggtttc cgttagcgaa aaaaataaaa
      121 gtaaagttat tccatgttat tggagattga aatttgaaaa taattgtccg ccattagaaa
      181 ttggaatttc gccgtattca ggtgatgtaa taaatataac ctgttttatt acatcagatg
      241 attttagaca tattaatctg aatttttcag ttgtttccga taaaagtatt caagtagata
      301 ctagaatatt ttctaaagcc aatgatttta atgacaatga cggaactgtt aaattctttt
      361 tggagggcaa aaatttattt tgtgtatttg gtgatggttc atgcaattgt aaagttgtgg
      421 ttaaaggtca gattggcttt atagttgatg atatggataa gatgattggt tttgctttat
      481 ataatttatc gcaatatcag atatcaatga ttaattcttt gaaataatgt agaaaaacct
      541 acgannnnnn nnnnctgacg gaaacatgat ctacggtccg cttaacggca aaatggctga
      601 gtttgagtac gactgccgca acagactcgt ttcggcgggt ggaatttcct acgaatacga
      661 cgctgaaaac aaccgtattt ctcagacgga aaatggagca aagaccgagt atgttgttga
      721 cagcaacagc agttcgctta cacgtattct tacggcggaa aaggagggcg atacaactta
      781 ttatatttac ggaattggtc tgatcgcaca ggaaaacggc aatgaatatt tgatttacca
      841 cttcaacaat atcggaagca ccgaggcagt gacaaatatc gacggagaaa tcgttgaaac
      901 ttttgattac ggaccttatg gtgagttgct ttctgagaac aaatgtagta taatgttcct
      961 gtataacggc gaactcggcg tagctacgga cagcaacggt ctgtactaca tgagggcgag
     1021 gtattacaac cccgagatta agcgttttat caatcaggac gttatgacgg gttcaatcgt
     1081 caataccccg actttgaaca ggtacgcata tgtcaacggc aatccgataa gcctgaacga
     1141 cccgttcgga ctaagtccgt ttcttaactg gcttgacgga attacgggac atgatattct
     1201 tgatctgctt ggaatgcttc cgggtattgg atttgtattt gatggaatca acgctgcttg
     1261 gtatgcccaa gagggagatt attacaacgc agcttgcagc cttgtgtcag cattgccagg
     1321 agcgggcgac gcattaggcg tgtttgcgaa aacaggaaag tcatgtaaac ttgtaactgc
     1381 attccataaa acaggatcag caggaaatct tatgatcggc tcatatgaat tgggaaaaac
     1441 ggctgataaa tacataagtg gagatgcaag tttcacatgg gaagagataa agggcgactt
     1501 atttaaagtt gcaatgacag gaacgtcaat gtggggttct gcaaaggact ttggcacatc
     1561 ttattgcttt gttgcgggta cgcttgtcac tactgaggac ggatttaaaa ctattgaaga
     1621 aatcgaagtc ggcgataagg ttctttcgga ggacgaaaca actggtgaag tagctgttaa
     1681 aacggttact gaaacttatg tcaatgagac agacgagctt attcatattg gtgtaaatgg
     1741 cgaaactatt tctgcaacgc cgacacaccc attctatgtt gataaacttg gttggacttt
     1801 agcacgttca cttcgtgcag gcgatgtact tgtactttca aatggtgaac ttgtaacagt
     1861 tgaatgggtt cagcacgaaa ttctcgaatc acctatcaag gtttataact ttgaggtgca
     1921 ggatttccat acttactttg tgggtcaatc ttcggtactt gtacataatg attgtgaaaa
     1981 atcaagatta cctcgaagta atggtgattg ggatggtatc cctggtgaaa gcaattggaa
     2041 atcatccaat aatgcagtaa ataaaattac taatggtgag tctttaccat ttaaaaatgg
     2101 acgtcctgat ttttcaaaat ggagtaaggg ttccttgaat tttaaaagtg gtgtcttaac
     2161 aggaactgat aaagacttta gattagtata taaagagata aagaaaaata tggaattcag
     2221 aagtcaaaaa gaggctaaaa agtggttgaa aaacaagggg ctaacaccac at
//
LOCUS       DAWEMR010000274         2185 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_15233_length_2185_cov_10.321596,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000274 DAWEMR010000000
VERSION     DAWEMR010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15233_length_2185_cov_10.321596"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          640 a          499 c          489 g          557 t
ORIGIN      
        1 agttataaca agcaatatta taacataaat atttataaat actatagaaa aaaggagaat
       61 tttttatgag cgcaaaaaac agcttcaatt ctatggatcc aaaagccatg caggcgcttc
      121 ttgcagtggc ttcaagaaag ttaggcacta ctcctgatgt gctgaaaggt cagcttgaaa
      181 gcggagttta tgacagggcg cttcatggta tgccacaaaa cgaggctatg atgcttcaaa
      241 aggctttgtc agatccgaaa actgccgaga aaatattgtc ctcaccacag gcaagggcga
      301 tattcaaaaa gcttcagggg aaatagtatt ttacagggca aaagcttgaa caggcggtga
      361 aataattgga cgatctgatg aacaaaatac agaatgtgct taatgacgag gaaagcatga
      421 agcagataaa ggagtttgcg gatatgctca cggcggataa cgtcggacag ggagaacagg
      481 gcggtcagag cgaagctcca caaagccctc agaacatgga cttttctcag cttctccaaa
      541 gcctgggcgg cgtttctcca cctgcggaaa gcaagcctgc gcaagcgaca cagacagggg
      601 gtcttgatat tgcaaagctt atgaagatac aaagcattat gcagtcagct tcaaagcccg
      661 ataaaaatat ccagcttctt cttgctctgc gacctctgct gaaagaagaa aatcaggtga
      721 aaatagacag gctcataaaa atattcaagc tttttgcggt gtatcccgca ctgaaagaaa
      781 gcggacttct gggaggtgat ctgcttggct tattctgatg acaataattt caccaccatg
      841 caggaggaag ccattcgccg tgtgagagaa atgcaaaggc gttcacgaag cattgtgggc
      901 gcaagcccta atagcgagcc tgcaccacct aaagcagagg aaacccctcg cactcagaat
      961 gctcacagcc cacaaaaagc ccctcatagc agttcgcttt tgagcggtct gcttgggggg
     1021 aatttaggcg atataaagat agacgaggaa aaagccctta tcggtctgct tatttacata
     1081 ttatacaagc agggcgcgga cataaagctt cttctggggc ttggttacct tcttctctaa
     1141 agcgtccatt caagaagtgc aagcatagaa atatcggaga aaatatcgga aagatagctg
     1201 tcatcatata tcctgtccat gttgtcacct gagtattcta tctccttgca aagctccttg
     1261 atggtttcaa agcgtgcggg agcgtcctgc tcacgactga ggtttatgac ctctgcggca
     1321 aaaacgaaca ctatcatagc ctttattttt gacagcaggt cgaaatctct cacagcttta
     1381 agaaaatatc tgtatacaaa atacaccgca agcttttcgt actcgccgct cgtctgagcc
     1441 tttaagggtg cggaaagctt tttcacaaag cggcttgcgt cctttatctg ctgtgggaga
     1501 atgtcgccta taaagtcaag acgttcaaga tataaaagca tattttcggc attttccaaa
     1561 ggcttttcag gaaaagcctt tttcaagctc tccacacgct ctgcaattat ctcagggtgg
     1621 gaaagcttct gggcaatggc ttttatctcc tctgtatcct cttcgtcaag agcatattgt
     1681 atctccgctg acgctgaaag aacgttcaca agcctgtcat ggagagaata ttccctgttt
     1741 tgaagcaggt caatgaaagc cgtccgtgca aaaagcatgg tttcaagaag ctggggatcg
     1801 aactcaaggt cgtcaggctc ttcatctatc tctttggtaa aaaaccttac aggctcatca
     1861 tcagaaagat acattctcac agcttcttcg caggcaagtc ccacgcctgc ctccttgtaa
     1921 tctccaaacc actcgtaaaa cctcgggtgt tcacggcata tgtctgagat gttttcctca
     1981 ccgtaagcaa gctgaacaga gcaaagcccc ttttcgttga gcatagggca ttttccgtca
     2041 cagtggacaa gatatttcac gccctcgtct tctgtaactg agttcacaaa cttcctgcct
     2101 atatcattgt caagcttttt gtaaaagccg taggtgtcct cgtctatctc gatatcccaa
     2161 tcggtcatac agcagttgtc agaac
//
LOCUS       DAWEMR010000275         2144 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_15543_length_2144_cov_11.049785,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000275 DAWEMR010000000
VERSION     DAWEMR010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15543_length_2144_cov_11.049785"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          645 a          377 c          571 g          551 t
ORIGIN      
        1 aagaacgcat ggagcgatgg tgcgcagtca cttactcctg cacagttcgc tgaaacaatg
       61 aagaaagtta aggcactttc cgagttcatg ggcaagcctc ttgatgtaaa cgaataaaca
      121 agacattgaa aaagaggtgt tttcgcacct ctttttcttt acaaatattc agttatgtgg
      181 tataatgttt tagaactgta aatggataaa gtacaaagga ggaagagcga tgaaacttgt
      241 gcttaaacaa aggctttttt catggtttga cagctatgat gtgtgcgacg aatatggtaa
      301 cgcagttttc agcgtaaaag gcagactcgc atggggtcac ttgctggaga tatacgacaa
      361 catgggaaac aaggtcggcg tgctgaaaga aaaggtgctg aaattattcc ctacctttga
      421 gatatacata ggcggagagt atatgggctg tataaagaaa gagttcacct ttttcaagcc
      481 taagttcgac atagacttct gcggctggag agtggatggt gatattttcg gctgggacta
      541 ttccgtttat agcggtgctg aacagataat gtatgtgtca aaggagcttt tgcaccttac
      601 tgatacctat gtcatagacg tttatgacga tagcaatgct ttggcaggat tgctcattgc
      661 acttgcgata gacgccgaga aatgctcaga cggcaattag gcttttatga tagctgtgta
      721 aaaaatcgta ctgaaaggtt ttgatattaa tgaaattcaa ctgcgagctt aaaagggtcg
      781 tggacgattc ttatgatata gaaataggca gaaaattgca ggatacgctg gtcagcgacc
      841 ttaaaggcgg tcttgtggga aagataaaga aatttgccgt tgtaacggat tctatcgttg
      901 tggatcttta cggcagagat atttatgaca ggctaaatgc cgcaggtctg aaagccgagc
      961 tttttgtgat acctgaggga gaaaagtcca aaaccaggca gatgaaagag tttgttgagg
     1021 actctatgct tgaaaaaggc tttcgccgtg actgctgtgt ggtagccgtt ggcggcggag
     1081 ttgtcacaga cctggcaggc tttgttgcag gaacatttgg cagaggagtg ccttttgtga
     1141 attatgccac aacgctcctt gcggcggctg acgcttctgt gggcggaaaa actgccgttg
     1201 atacgcccct tgctacaaat cttatcggtc tgttcaatca gcccaagaaa gtgtatctcg
     1261 atatagacac atggaaaaca cttcctgaaa aacagctttc aagcggcctt tcggaaacta
     1321 taaagcacgg ctgtcttggt gataaggagc tgtttgagta tcttgaaaaa aacgtggaaa
     1381 aggtgcttga atgtgacggt gaagcctgcg agtatatcgc aaagaaaaac tgcgaagtca
     1441 agtacaaagt ggtaatgaaa gacgagagag agtcgggtct gagagaggtg cttaatctcg
     1501 gccacactgt tggcagagct atcgaaactg tgtctgatta tagactatat catggcgaag
     1561 ctgtagctat cggtatggtg gcacagtcaa gattgggcga gaagcttggc tttatatccc
     1621 atgaaaatga acagcgtgtg gaaaagcttc ttgaaagggc aaagcttcct acaaaaatac
     1681 ctgactatat tgacagaaag gctcttgtga aaaagcttta tacagataaa aaggtccgtg
     1741 acggaaagct gagatttgtt ttccaaaaag aaataggcga gatgatgtgc ttcggtgaaa
     1801 atcagtacgg caaagagata tccgaggagc agattgctga aataatcaca gagatgtaac
     1861 ggaggatagc gatgaacata actgtatttc cgaaaaaact cagcggacag gtgacagttc
     1921 cgccctcaaa aagcgttgca cacagaatgg tgatagcggc ggcgctgtca aacggcattt
     1981 ctgagataac aaatctttat ccctcggtgg atatccttgc gactatggat tgcatgaggg
     2041 cgcttggggc gaaaatagat tttgacggcg acaaggctgt gatagagggc ataaaggata
     2101 ttcctgagag cgcagttttg gattgctgtg aaagcggctc aacg
//
LOCUS       DAWEMR010000276         2085 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_16032_length_2085_cov_12.112808,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000276 DAWEMR010000000
VERSION     DAWEMR010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16032_length_2085_cov_12.112808"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          547 a          394 c          489 g          655 t
ORIGIN      
        1 gggcgcaact gctctttcac gctctgacag cgtaacagat gaggagtttg agcttgtttg
       61 caatatattt ggctcatgcg gtatgtatgc cgtcatatca aaggataaaa tgaaagagat
      121 aatagcaata aacggctcat ctcctgcttt tatctatctg tatgctcagg cattcgtaga
      181 gtatgcgaag agcgttgaca tcgatgaaac agttgcaaga gatctttttg caaagtcgct
      241 tatcggctct gccaaaatga taactgacag tggcaagtca cttgacgagc ttattgaaat
      301 ggtatcatca aagggtggca caactatcgc tggtcttgaa aagctccgtg agggcggtct
      361 gccaaaggct gttgaggatt gctgcaaggc ttgcactaag agagcttacg agctttcaaa
      421 atagttattt tttctgcacc gtggcatata cattactcaa aggagtgatg tataatgaac
      481 agcgggacag gcatatacaa aagcggagtt cttgcatttt ttgcaggagc tatcctgatt
      541 ggtgcgcttt tgggtgctgt tactttttgc tttatgggca gcgacgaact ttcaatgctg
      601 tcggtgtttt caaacagctt tgttgacgtg aggtcgtcag gtgatcttga agaaattctt
      661 gtgaggtctt ttttcagttc gactgttttt ttagcagttg tgtttttgtc aggtttttct
      721 tcactgggac agcctttagc tgttgcagta ttgctgttgc gtggcacggg ctggggtgtt
      781 gcaatgtctc agctttacag catttacggc ggacatggtg cattactttc agcgatgttt
      841 gtacttcttc ctgcagtcat aacagcggcg gcacttatga tagccgcaag ggaagctgtc
      901 gctatgtcaa atatcctgct gcgcattgtt ttttctgaag gacagcatag cggtctgcga
      961 cagacggtgc ggctttatgc tgttaagttt cttgtgcttg aagcagcggc ggctgttgcg
     1021 gctgcgcttg aatgtctgct gacgattatt tttgcaggac gaatttagta tacagtatac
     1081 aaagaatgaa ataaaattcg gggcaatttc ggggactatt aattttaagg agcggtgtaa
     1141 tgtctttatt tggtaattac aatacccctg gcagaggtgt gcttaaagca cctcaggaga
     1201 aaaagggtat ttttaagttt tttgaagtct atggcaggca tatgtggaag cttatggagc
     1261 ttaatttgct gtatttcgtt ttctgcatac cgatgacgct tatggtcata cttatgctta
     1321 tgacatcaaa tccaatatgg ctcttactgg cgataccgtc agtattggta ggacctgcaa
     1381 cggctgcaat gacaaaggtt tgccgaaatt attctcagga aagaaatgcg tttctgctac
     1441 atgatttctg ggattctttc aagaagaatt tcaagcaggg cactatcatg ggtgccatag
     1501 atatcatctt cgctatagga tttatggtcg ggattccaat gtataaatat tgggcagagc
     1561 agaactccat gatatacatt ccttttgtta tttgcataag ctgtcttatc gtattcttca
     1621 tgatgcactt ttacatctat cttatgataa gctccactaa ccttaacatg aagcagataa
     1681 taaagaactc gttttatctt gtgagtcttg gcataaagca gtccctttgg tcacttctgg
     1741 catcgcttat cgttattgtt atgatgtatc ttttcctgcc gtattctctg tttatattgc
     1801 cattttggcc tctttcgttc atatgcttcg tgacttgctt caattgttat cctgttatca
     1861 gaaaacacgt tatccagcct tactatgatc agcgtggtga aagcaatcct gagtttgcat
     1921 acaagaatgc agaccctgac gaacagctct ttgaggatag agcggcggaa gaaactcctg
     1981 tcaagaccaa agagagcaga aaaaaaggca aaacaatctc ataaaaacat aaggcgctgt
     2041 aaaaaaacgc gcaataaaaa aagactgcaa ccacccttaa aaggg
//
LOCUS       DAWEMR010000277         2050 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_16326_length_2050_cov_9.289724, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000277 DAWEMR010000000
VERSION     DAWEMR010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16326_length_2050_cov_9.289724"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          505 a          484 c          413 g          648 t
ORIGIN      
        1 gatagttctt ttttgctgtg tgatatatta aagcccgtgg gcttccatat cgaggaaatc
       61 cacgggcttt gttgcgcttt ttttacagcg ccttttttat gatactaagg tcatttgtcg
      121 ttttgcggaa gcgtaacaat aaaatttgta ccctttccgt attgtgattc tacacggata
      181 ttgccgttgc acagctccac gatacgctta acaaggggca aaccaagtcc gtttccctct
      241 gaagcgtgcg ccctgtcccc ttgataaaaa cgctcgaaaa tgtgtgatat ggtctgctga
      301 tccattccga tgccacggtc agttatctca gccgttatca catcaccctc tttaaaaagc
      361 tttactctga tagagcttcc ctccggggag aattttatgg cgttgcctat tatgttcacc
      421 cacacctgcg aaagcatttc cgcattggtg gtgtattgta tctcctgcat atcaatgtca
      481 aagcttatgt ccttagccgt ccattgcttt tccagaagaa gtatgcagtt tcttatctgc
      541 tcgtcaaggg aaaagcttgt cttgtcagtg atgatctgct gattttcaag ctttgaaagc
      601 ataagaatgt tcgatgaaag attggtcaaa cgctttgatt cgctgatgat aatgtcaatg
      661 tattcctgct ttttctcctc cgagagattt tcttttttta gctgtttcgc aaagccttgg
      721 atagaaacga taggcgtttt gaactcgtgg gagaagctgt tgataaaatt ctccctgaac
      781 atctcgatgc ctccaagctc ctgcgccatt ttgttgtagc tttctataag ctcgtctatc
      841 tcgccgttaa agcctgttac ctctaacctt tgagagaaat ctcccttttc taccctgtgc
      901 attgcctttt ttacctcacg cacaggcctt actatcttgc ttgctacagc ggcagaaatg
      961 caagtgccga ttattacaga taccacaagt cccacaataa ggaaaacaaa aggcatttgc
     1021 aaaggggata gaaaatgcag gcatcttttt atgcctatca ttataaatcc cgtaagaagc
     1081 cctgcccata tcattatcat aaataccgcc gaaacaatgc tagcgttcat ggtgtaattt
     1141 cgcctgcgct ctgagcgaag agcagagata agatgttttt tcatgagcct atcctttcag
     1201 cttgaccgcc ttgtagccaa gccctctcac tgtcaatatc tcaaaatcgt ggttattctt
     1261 caaatgctct cttatgcggt ttatgtgtac gtccacagtg cgctcgtcag tgtctgaggt
     1321 catatcccaa agctcgtcca taagctgacg gcgggtgaat atcttgtcgg gataggaaag
     1381 aagcttgtaa agaagcatga actctttcgg cggaagctcc cttatgccct caggggtctt
     1441 gatagtcagt gcgtcatagt cgagagtggt gtcgcccaca gttatcttgt ggtcgctcac
     1501 tatctgcgaa cgtcgcaaaa gagcctgaat acgcagaagc atttcctcct cgtccacagg
     1561 cttcaccatg taatcgtccg taccttttat aaagccctgc tttttgtcaa aagccgtttc
     1621 cttagccgtc accattaata taggcgtctg aaacccagcc ttgcggatag tttcgcaaag
     1681 ctccaagccg tccatacgag gcatcataac gtccattata ataaggtcaa cgtgtttttc
     1741 gtccattatt tcgagagcct ctataccgtc acgagcctcg ataggtgtgt agccgttgtc
     1801 gctaagcaca tcacaggtca gtttgcgagt gttagtatcg tcctccacca caagtatgtg
     1861 aaacattttt ttacccctcc gttcactttg cattttttta ttatatcacc gttttattaa
     1921 cgtgatatta acgatgtcaa gacacattta gttcaaaatt ccatgttgat cctgcacaat
     1981 ttcaggggta ttgtcttgtg caaacaaatg atttatattt taatttggca acatttctgc
     2041 aattgttgca
//
LOCUS       DAWEMR010000278         1944 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_17392_length_1944_cov_7.755956, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000278 DAWEMR010000000
VERSION     DAWEMR010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17392_length_1944_cov_7.755956"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          586 a          400 c          438 g          520 t
ORIGIN      
        1 agcggcagat cctatgtcct ctcagtaagc gtcggtgcag cagtagcacc ggtggaaaac
       61 ttctggtttg acgatgtaat gcacatcgcc gataaatata tgtatgaaga taaaagacaa
      121 aaacctcata acagaatatg atttgtaatg gttctgaaaa aacttttatg ggcgaccttt
      181 ggccgccctc tttttgtcat tgaccggtcg gcaaaaattt tcatacaccc tcagaggacg
      241 gataaaaccg tccgtttttt attttttgca taattttgct tgacaaaacg acataaaagt
      301 tgtataatat taattaatgc tttatgaact ttgtgtttaa gcaccatgta caaaaacagt
      361 gtcatatatt tgtgcgaaag atagaatttt gtaaaaaata gcaacatttt gcctttttaa
      421 aacgtactta tatatagtag gctcactttt tgcagtgttg cccacaagaa acttttcaga
      481 acggaacata aaaaatgaag ataaaacagc ttttttcggg tgataccgat aatacattca
      541 tacagttttt cagatacgtc tttgtgggcg gctttgcctt tgtggtggat tacggcatta
      601 tgaccgcact tgtggagctt tgccgcttcg accccgtcat tgccgcgact atctctttcg
      661 tggcaggact tttggtgaat tatgttctga gtacgttttg gatattcaaa aattcaaaga
      721 taaaaaaccg ccttgcagag tttgcggcat ttgccgccgt gggcgttgtg ggacttggca
      781 taaacgccgc aataatatgg cttttcaagg atatccttgc ttcaaagctc atattcggca
      841 cgcttctccc gcaggataaa tattatcttg tgggcaaact cgtttccaca gtgatagttt
      901 tcatatggaa ctttgcaggc agaaaattta ttatatttga taaaaataaa gactgaacag
      961 gagaggataa cacatggaaa aggtaattat tataggcgca ggcccagctg gtctgactgc
     1021 ggcaatagag cttcttaaaa acggcggtga ctatgacgtg accatactcg aaggctcgca
     1081 ggcaatgggc ggaatatcac agacagtgag atataacggc aaccgcatgg atataggcgg
     1141 acacagattt ttctcaaaaa atcagcaggt catggattgg tgggcagacc ttatgccatt
     1201 gcagggcaag cctgcctatg acgataaaaa gctcggccac gaaaagcccc ttgccgaaaa
     1261 cggccccgac cctgaaactg aggataaagt aatgctcgtc cgtgacagag tgtcgagaat
     1321 ttattacaat aagcacttct ttgactaccc tatctcaatg aagcccgaaa ccataaagaa
     1381 catgggcttt gtcaccacta tgaaagcagg ctttagctac ctttgggcgt gcgtgttcaa
     1441 aaagcctgaa acaaatcttg agaatttcta cataaaccgt ttcggaaaag tcctctattc
     1501 aatgttcttc gagggctata ccgaaaagct ttggggcaga cacccaagcg agatatccgc
     1561 agattggggt tcacagcgag taaaaggtct ttctatcaca gcccttttga aagatatgtt
     1621 caaaaaggtg ttcggtacaa agaataaggg cgaggtagaa acctccctta tcgagcagtt
     1681 ctggtatcca aaatacggcc ctggtcagct ttgggaaacc gccgctgaga aagtaaaaga
     1741 gcttggtgga aaaatactca caggctgtca ggtaaagaaa ataatgtgcg agggcaggaa
     1801 agtcacctcc gttgtctgcg ataccgctga gggcgaaaag accttcacag gtgatatctt
     1861 tatctcctca atgcctgtca aagaccttat cctcggcatg gacggcacaa agccttgcga
     1921 taccgtcata aagatagccg aagg
//
LOCUS       DAWEMR010000279         1937 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_17477_length_1937_cov_6.358130, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000279 DAWEMR010000000
VERSION     DAWEMR010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17477_length_1937_cov_6.358130"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          654 a          357 c          362 g          564 t
ORIGIN      
        1 tatcgataaa tagatatcga ccaaataaat ccaaagacat agaggtatat agtatagagt
       61 atgggatatt aaacctatga gtgtgtgaat aagaataatt atttgagttt atgtaggttt
      121 taattatttt ctcaagataa tcatttttaa aattgattcc tgttatctca cgaatatctt
      181 taatgatttt agatttattt ttatttatta atgatagtaa atttgaattg taaacctcat
      241 catttctgag ccgtctgcaa gaaaaagaac tagctttatg caaaggacaa cccaagtact
      301 tcaattgatc atgtttgggt aatcctaaaa tattttttcc agtatgtctt gcatgcgtat
      361 tacgtttttt tgttatttgc tcttcatatt cttgcccaaa caaattaata atttgaatgg
      421 ggtttccaca ctctggacat attgcatagt gcttttcttt tcctttagta tctacctgaa
      481 aatatggctc ttttttatga gtagcatcaa caaagttatc tattgtaatt tcaaaaggac
      541 ctttagtaag tgtactaaat acattcataa acgttcacct ctaatattct aaactcatta
      601 ttatcatatt actatattgc tgaaaaatca ataggcagta tatgtcctgc caagggggca
      661 actgcaccca tacgctgact ggtgcctcct gtcactgtga taagacaaga actacccaat
      721 ataagatatt acagcagaaa gaaaatatat taagactagg aagtgataac aatatcaaca
      781 tacattcatt ttacaaaaga acagagagaa cgggcaaggc gaactgacct tgccaatttt
      841 ttaatcagtc agggtgagca agtcaagcgt tcaggctaag aatacgaatg gcttgacggt
      901 tcgcaaaagg tcacaatcag aggtcatctc tggtatcatc agtacgaaca aaaaggcggt
      961 gatgcggttg acttcgtccg cagattttac aacaaggact ataccgaagc ggttcaaatg
     1021 cttttgaata acggtgacag tcaaatcata acatcaccgc ccatagaaaa agagcataaa
     1081 ccctttaaac tgccaccgag gaatgacaga atgagccgag ttttttctta tctgctgttg
     1141 actcgtggta ttgataaaga tgttttgtat gagttcgtgc ggcggaaaat gatttatgaa
     1201 tcgtcagact ttcacaacgc tgtctttgtc ggctacgatt caaacggtaa acctcgccac
     1261 gcccacaagc gaggaactgt gacgaacaat ccgtacaaag gaaatgctgc aggaagtcaa
     1321 cccgaattca gttttcactg gcacggcaca agcgacaaga tattcttatt tgaagctcct
     1381 attgatatgt tatcgttcat aagtatgcat aaggaaaatt ggaaaaatca tagctatgcg
     1441 gcatcctgct ccgtttcgga cagggtgctt tttcaatgtc ttaaagacaa tccaaacatc
     1501 aaaaatgtgt ttctctgctt tgacaatgac gaagcaggac agaccgccaa caagcgcata
     1561 gccgaaaaat taaattcaat gaatatccaa aataaaatcc ttgttccaac tcataaagac
     1621 tggaacgaag atattttgaa aaatgaaagg actgaagaaa tatgccatca ggtgttataa
     1681 ctcttattat tgtgacaggc tcaatgtttg cccttttcat aataatttct acggtgggca
     1741 attattattc cctcaaccgc ataaagaaca agacagtcgg gcaaggtcag cacggtacgg
     1801 ctcgttgggc gaccaaaaaa gagataaaaa gaacctacaa gcacataaat tttcagccta
     1861 acaagtggag aaaaaatccg aatagcagac ctactcaaca gggcattgtt gtcggttgta
     1921 aaagcaggac aataggt
//
LOCUS       DAWEMR010000280         1926 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_17581_length_1926_cov_7.125601, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000280 DAWEMR010000000
VERSION     DAWEMR010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17581_length_1926_cov_7.125601"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          525 a          431 c          373 g          597 t
ORIGIN      
        1 cattcgggct gatgacattt cgctcttgtc atcatagaga agtctgcatg atctattctc
       61 acgaggttag tagctactaa ccacatttat tttaccatgc gtgaagccgt atgtcaatct
      121 tagttagtca tatctaatat attttatagg tatgcacaaa aggtgtactg cctgcaggca
      181 tggctattga ctttttgaac aacagataag ctttgcgaaa cttttcgttt gttattattg
      241 acaagctttg tgagaatatt cgttttgggc gtgatcttat gtatgctcaa aaccgcacaa
      301 agcatagtag ggtatagaaa aaagcggtgc agagattgtt ctccaacacc gccttgattt
      361 atttctcatt taactctgca agtgcattcg ccattcttag attgctatct gtggttatct
      421 tgaaattcaa tgcgtccccg acaacaaggt agatatcctc attgcaggcc gtaaaaacag
      481 aacaagtatc cgtcagcttt ttattttgct cctcgctaag cgaataataa ttcctcctca
      541 gatccccaag tctgaaagtt tgcggagttt gcgcctgata aagctcgctt ctgttaagcg
      601 tttccctgac cttttcgccg tcagatctta ttatggtatc agccgcaggc atataagttc
      661 cgcaggcgga atgtttcgcc attgcctcga tattgtcctt tattatcttc tctgtcacga
      721 atggtcttac agcgtcatgg gtgagtatta tgtccccatc attctcacca tgttcagcga
      781 ctattgcctc agctatgttc attacagtgc cgtttctgtc agctccgccg tctatgactc
      841 ttacacgctc tttgtctatc ccgaagcgtt caaaaagctc gttagtgtat tcgtgccagt
      901 caggttttat gcccacataa accatatcta tctcctccac tgcaagaaac gctctcactg
      961 ttctaacgat aacaggcact ccgcctagaa cagaagcgta gtacatattt ctgttgatag
     1021 cgccctcgtc aggggagatt atcatgaagt gatccttgtc aggtctgaaa tctgaaaggt
     1081 ttttaaacat tgccttgaga acctgatagg aagggattat gttgtcaaga cccatgagtg
     1141 gaacagcatt ctgtactctt gggtcgtgag cgtcaaaagt aaggatattt gaaacgccca
     1201 tagactgaag ctcctgaaga gcaactgcac agtcaagaga ttctctgtag tttcttctgt
     1261 gctgtctgcc gccgtaaagg gaaggcatga tgatgtttat tctgtgagcc ttaccgcctg
     1321 cagcctggat tattctttta agatcctgat aatgatcgtc aggcgacata ctgttttccc
     1381 tgccgaaata gttgtatgtg cagctatagt ttcctacgtc gcagagaatg aaaagatcgt
     1441 cgcctctgat agatgacttg ataaggccct tgccgtcgcc ggaagaaaat ctaggacaaa
     1501 cagcctcaac aaggaaagta tccttaacaa attcgttttc attagcccat tttgtaagat
     1561 aaccgtctac cttcttgcca agctcggaag cgcctgccat agcaataaga ccaagcggtg
     1621 caactcttga gtcattgtcg aacaatacat tgtcagcatt acccattatt atcatatcct
     1681 ccaatactaa ttgttgtctg acctcaggtc agtacgaaac ataccaatat acatatatta
     1741 tactttataa atcccgattt tacaatagct tttttgcaaa taataaattt ttctcacttt
     1801 tcacaaaaaa cacactgtta ctgcggcaca tttagacaca aaaatcaagt aaacgtttgc
     1861 tgaccgcacg acaggtaaac tttttttgca tatataaaaa agtccttgcg tttttggata
     1921 aaatga
//
LOCUS       DAWEMR010000281         1910 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_17737_length_1910_cov_5.659299, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000281 DAWEMR010000000
VERSION     DAWEMR010000281.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17737_length_1910_cov_5.659299"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          523 a          457 c          278 g          652 t
ORIGIN      
        1 gaactccttt ccttccgctg taataaattc cataaggttg cccatcccca tagcaccagg
       61 ttcggcaagc gtaaagatcg ctatgtcttt aaacatttct tttgtatatt cttgattttt
      121 tacgtttgcc aatttcatat ccacctttca tatcactatt cataatcata tggttctaat
      181 ggtatttgcc agcttttgga tagagtgtct cctcatcatt aaaagctttg atagtattct
      241 gtatctgctc ctccgaaaaa ccaaagtacg ttatctcata caacaccagc gccgcaaatt
      301 tgacattgcc atacgttgaa aggcttgctt tatcgactgc ataacctaag acctcatccc
      361 aaggaactgc ctcgatactg taggttttgt tatctgatct atcgagcaaa taaacgttgt
      421 aaagattttc accttcgtcc tgtcggtctt tcagttgttc aatgaataaa acttcattgt
      481 cagacttctt tggagtatat ttttattaac tgaacacttg tcttgacatt tgataatcgc
      541 taaaaattcg ctgacaacct taaattcatc gcagctttgg agcacttgat aaaagtacat
      601 catatctccc ccattatcat tctgaatcat tcatgtcaat aacataatca tttatcgaaa
      661 gccacgccac tatattttct ttacccatat agaaacaccc ctgtttgaaa tatcctcgtt
      721 cctcccctag gctgtatgcg tccgaatgcc aagttgctac gtctaacccg ccattcttat
      781 atagcacgac acagcctgtc atatcctctg gctttttctc ctttaagttt ataaacaccg
      841 acaatggaat attcaacttc ttctttgaca taaaaataac ctctcctctt ataatcacat
      901 atcctacaac tcattcatat agttttgtgt gtatttaccc caacagctta gcatttcatc
      961 atctgaaaac ttcccgtcat atagatagtc cttcaatttt ctttccgcct ttacaatctc
     1021 atctaggttc gttatattct ttgggtcgct ctttattact tcaaatattc ttttcaaagc
     1081 atgtgtgtag cccttttgac cataggattc tctaccttct tcccttatct ttggttgtaa
     1141 attatgtgtg actatgtgtg aaacagtttc atctatccag gacatctggt gattaaaaga
     1201 cattccaaaa aagctatcct gaacacgacc gctttccatg ctgcccactc ctcgtctttc
     1261 atacgcatct tctctatatt tatagtataa cacgctttta ccgttacgtc tagaacgtta
     1321 tttatcacat atcaaactat ttataatcca gcaaacggca cttatagtac cataataatg
     1381 accacggtat aatcaaaacg agcgtacacc aaatcggtat acgctcgttt tgtcaaaaag
     1441 ctattactct gaaatatatt tttcagactt ttccttgaat ctctcgtgaa tttcttgatt
     1501 tctttcttcg acaacttcgt caataagctg tgaaaatgta ttattgatat ttaccttgag
     1561 aagttcctgc gtctttgttg acagaaaata atcaaagcat atctcggaaa tgcatttgaa
     1621 aatatgttcg tatttttctt caggcttcat actatcccag cctttttccg cacactgcga
     1681 aatcatagct catatcgtcc ctgtcaggag aaccgtagcc aagctcctcc ggaacatcag
     1741 gcacgccctg ctgtgcttcg ctttcaaaag ggggtggaac aaactctgct ttgggctttg
     1801 tctgcggtct gcaaagtgca tatatcataa caagcagcgt caccgcaaag caaaccgcca
     1861 gcacagatac tctcaatttg ccgtcatctt ttttgcttat tcatcatgag
//
LOCUS       DAWEMR010000282         1904 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_17796_length_1904_cov_9.045430, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000282 DAWEMR010000000
VERSION     DAWEMR010000282.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17796_length_1904_cov_9.045430"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          552 a          350 c          458 g          544 t
ORIGIN      
        1 gaacacagcc gacgggcttc gctttgcgtt gccgttttat tcgttgtgat ctgatatatc
       61 tgcccgtgtg tgacctgtca tgcacgggca tttttatgcc tgtttgtccc tcacgtcata
      121 aaaaaatgaa acggaggaca agcaaatgaa gaaaaatgaa ctgaacaaaa atgtaatttt
      181 aaacgaggac gcagtaaaga acattcactg cgacgagata accgctgtca tcaacagcaa
      241 tgattccccg aaagccatga tgaacaagct tgacgattat catggcagtg atatcgcaga
      301 ggtaataagt acgttttctg ttctcaacag aaagaaattc taccgtgtgt gcagtcttga
      361 aatgctggca gagatatttg aatatcttga cgaaaagcag gcaggggagt atctgagaga
      421 aatggatatc cacaaggctt cggagcttat atcggagctg gacactgata cagcggtgaa
      481 tattctgtct gaaacagata aggacaggag aagccttatt atagacgctg ttgcacctga
      541 tgtacgcagt gaactcaagc ttatcgcttc atttgatgag gaagagatag gaagcagaat
      601 gactacgaac tgcattatca ttgacgaaga catgactgta aagcaggcaa tgaatgctct
      661 tgttagtcag gcgagaaaaa atgacaacat ctcaacgctg ttcgtggtgg acgaaaataa
      721 ggttttcagc ggagctatag atctgaagga tcttatcacg gctgacagcg aggtggagct
      781 tgagagcata acggcaactt cttttccata tgtttacgcc aacgagcaga ctgccgattg
      841 tatcgaaaag ctgaaggatt attcagagta tatcatacct gtgcttgacg attcaaacag
      901 gctgctggga gttatcacag cgcaaaacat catcgaggca tcagatgatg aaatgggcga
      961 ggattatgca aagcttggtg gtctttctgc agaggaagat cttaacgagc ctgtgttgca
     1021 gagtgtgaaa aagagattgc cttggctgct tgtgctgttg ggacttggca tggtggtgtc
     1081 cacagtggtg ggagcgtttg agaaagttgt tgcacagctc actcttattg tggcgttcca
     1141 atcgctgata cttgacatgg cgggaaatgt tggtacacaa tcccttgcag tgactatccg
     1201 tgtgcttaca gacggaagcc ttactgcaaa gcagaagctt catcttgtct caaaagaaat
     1261 gcgagttgga cttctaaacg gcactcttat aggcgtgctg tcgttcatag cagtggggct
     1321 gtatattttc ttcttcaagg gcaagggact ggtatactcc tttgcaatgt cgggctgtat
     1381 aggcatttca cttatacttg ccataatagt atccagtgca gtgggaacgc tcacgccgct
     1441 tttcttcaag aaaataaaga tagaccctgc cgttgcgtca ggccctatga taaccacgct
     1501 gaacgatctg gtggcagttg tggcatacta tggaactagc tggatatttc tgctgaatgt
     1561 tttgaaactg gggtagaaaa aatagaataa gataaaaaag ccgatcgcat ccatttgggt
     1621 gcggttggct ttttcaggtt tggctattag attcaaggta atgcctatct tattgaataa
     1681 ttttgcattt ggagttttct tcaatttctg aaaagacctt tttttgatcg attattgaat
     1741 catagtatga cttccttatt tctttgacca aaactaattt gttattctca atttttttga
     1801 attgataaat gccatagatc ttatggttga aaaagtaaat gaaagatacc agtttttcat
     1861 ctgacaaaag ttcaccattt tgtgatttta cgaaagatat gatc
//
LOCUS       DAWEMR010000283         1889 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_17960_length_1889_cov_10.087241,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000283 DAWEMR010000000
VERSION     DAWEMR010000283.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17960_length_1889_cov_10.087241"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          629 a          345 c          473 g          442 t
ORIGIN      
        1 atatgacgat gatagaaaaa ctcagcgaaa gtgcttgtgc agacgatgaa ctatgatact
       61 aaaaaagttt agaaagcaat ggtggacact atggcggctg tcataatgtc agatgatgag
      121 aggtgaaata tggataaaaa ggtaaaagct ttttttgcag ctatgggaat tgccacggcg
      181 ttgttagtgc ctgtttctgc atctgcggat aattctgact caaaagtgca ggtgagcatc
      241 gacatcactt gggactcttt agagtttacc tatacagacg ggcagtggga tcctgagacc
      301 cactcatata aaggtggcgg ttggtctgac agcggcggaa attttacttt gacaaacaca
      361 gggaatgtgg gtgttatggc ggatttttca tacaaacaag cagagggcat ggacgagata
      421 aaaggctatt tttcaagcag tgagctaata gtgcctatct cagaaagccg aaacagcaaa
      481 cttactttgt caggtaaacc aacagagcat tttgaaagca tgacggtagg cacggttaca
      541 gtgaatgtct atcctcacgg ctgggcaaac gtgaacggca caaagtatta ttttcgactt
      601 ggttataggg aaactggctg ggtaaaggat gaggacactg gtgattggta ctattttgat
      661 aaagacggca atatggtgaa aggttggttt aaggacggcg gagaagaatg gtattatgcc
      721 gacgaagacg gcaaggtgct cacaggcagc aacaatggtg gtggaaacga ttggtattat
      781 tcttatgctg acggcaaatt gtataaagat tgggtcaaag tggatgacga ttggtactat
      841 tttgacgtag acggctcaat agcaaaggac accactatcg acggacggta tattggactt
      901 gacggcaggt gggacgggat aggcgacgca ccaaatatgt aagtaataaa aatcaattat
      961 aaaaaaggag aactcactta tgaaaaagac aatttctatc acaatggcat tagcacttgt
     1021 ggcaacactc agcacggcgg cgtttgcagc ggaggctgat gacggaaaca tcaaaaagaa
     1081 tatcactgta aaggcaaagt atgttgagga tatcaagact tcaaaaacta tcagtgccga
     1141 cgttgtgtgg ggcgaaatgg agttcacata cagcgtgaac ggaacaaaga cttggaacgc
     1201 taaaacccac gaatatgaca ttgataccaa gggcgaatgg tctgcaaagg gcaatgatat
     1261 aagcgttaca aaccattcca atgcggcaat cgacgttgat cttacatacc agcctcttga
     1321 tgaatacagc gttgtaaaag gcacattcac aaatgacgaa tttaccatac ccacagccga
     1381 gggcaaggct gttgatgatg aaagccttac agtaagcacg gctcttaccc taagcggcga
     1441 gctttcatca gatgttaccg ctcttacaaa agtaggaaac gtagcggtaa atattgcaga
     1501 agctttggaa aatgcagata cagatgtaaa aacagtttca tcatataacg accttgttag
     1561 cgcagtggca gcaggcggaa agataaagct tgatgacgat atcacactga aaagccgcat
     1621 aaactgcaaa aagaacactg ttatcgaact cgaccttaac ggtcacacta tcacaggaca
     1681 gataatgaac aatggcgcag attgtactat caaaaacggc gctcttaacg gcgacgaagg
     1741 tccgataatg gtgcagggcg gcacaacaaa tcttataggc tgtaagatat ccacaaaata
     1801 tactcctgtt tatgtttcga ggggtactgc aaatataact gactgcactc ttacaaatga
     1861 agatgcgaat aaaagcgtag ttatcaata
//
LOCUS       DAWEMR010000284         1889 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_17962_length_1889_cov_8.781352, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000284 DAWEMR010000000
VERSION     DAWEMR010000284.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17962_length_1889_cov_8.781352"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          571 a          417 c          357 g          544 t
ORIGIN      
        1 gtctgactgt cagccaacct atgaccacaa taaagaatat attaagaaca tatttcaggt
       61 tcttttttat tttcatcatc tccgaatttt cagcctgtcc attaaaaggt cagaccgttt
      121 ttgtcccaca catatttttt acagcacaaa tgccataaag cacagattta ctattatctg
      181 caaaattgta aatcttatta ctacctgagt atcagaccag cccttgttct ttcttgcatg
      241 gtcgtgaaga ggagttctta tgccctccat aaaattcttt attttcagga aacgtctgaa
      301 agaaagcttg agaagtccaa gtccgccgtc aagtatgata actatagcca tagggataaa
      361 tgcgaacggt gcggcagttt tcaaaaacat caccgcaagg aaaacgccca gcgctctcga
      421 gcctgcgtcg cccatgagca tctggctagg cgagcagttg aaccacagat aagccgcaag
      481 cgtcacgaac attatcatag gaaggagcat atcagccgca ccactctttg tcaccataaa
      541 tgcaagtggc agaagaactg tcatgaccaa tgaaccgcat agcccatcaa cgccgtctgt
      601 acagtttgta acgtttatag ctgtccaaac gaggacgcct gcaaggatga tgaacagcgg
      661 tgcagggata gtgaaagtgg aatctgtgat ataaagcttc acctgcgagc cgttataaca
      721 atagtatgta aacgcaatgc caagagatat aacaaggtcg ataaggccct ttttcaggtt
      781 gccccaagga gtttcagcag catcgtcaaa atatccgctg agcatggcgg catacacaag
      841 cacgagatag attatgttct cgatatcgag aggataaaaa agcgctgtgc aaagtgtaaa
      901 ggaagtcacg aagataattc ctgcgccacg aggcttgccc tcagaaagtg cgccgttcac
      961 agcaaacgct ctgccctgat ctttaggcaa aatgcgtttg aagaatttca ggctgaaaaa
     1021 agcaataaga aaactgcaca gcattggcac aaatagcacc aatgtagtat tattagtctt
     1081 agccaaaagg ttataaaaca ttgtcatcgt ccttttctgt ttcaattcat acatatacta
     1141 ttatattata caacatagct gtgcaaaatg caaatattat tatccgtcat tttgcacaat
     1201 tttttcttaa tggtttctta atgtagaaaa tttaaggtaa gtgccaagca aaacaaaatg
     1261 cacagcccat ataagactgt gcataggttc attctgtttc attcaatatg ttcatcattt
     1321 cggacggcgt aacaataaaa tctttcatgg gataatgttt cttatttccc gtgacaacat
     1381 aagcattatt atcacgcttt tccattgcta cctcgtagaa aataagatcg tccatatcca
     1441 cgagtatctc ccctgtcggt ttcgggtcaa ctttaattcc atttgaaata atagaattta
     1501 tcattgtttg gatagtttca gcttttaggt gaaacttagg tctgttcaag acttccgcat
     1561 attcatctat tatgtcatca tgaaacagcg gcgttatcct gccgtcaagc acctcacgaa
     1621 gcaccataca cggcggagat tgcgaatttt tagtgataag agcagatacg ataacatttg
     1681 tgtcaataac agcgcagact ttcatttatc attttctcct gttctttctc acttctgata
     1741 tctcagcgtt tatctcgtcc aaagacattt cagggacgtc agttgcctgc tctctaagct
     1801 tttcaaacgc ttcaagtccc tctgctcttg taacagcatt ttcaggaaga actgtttcaa
     1861 atggtattcc acgcaccatt ttgctgcgc
//
LOCUS       DAWEMR010000285         1858 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_18263_length_1858_cov_9.784803, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000285 DAWEMR010000000
VERSION     DAWEMR010000285.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18263_length_1858_cov_9.784803"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          597 a          346 c          475 g          440 t
ORIGIN      
        1 agaaaaagcc tgagaacgct cagaagccca gcagggtaga ggcgactata aatgttagag
       61 tgcctgttca gaaagagcag gagcctgtca ggaaagctga aaaaaaggct gagcctaaat
      121 ctgagccgaa acctgatcct gtggaaaaag aagaaagcga cgctaaggca gaggaagctt
      181 acagaaaagg catcgaaaag gcaaaaaaac gtcgtgagga acgtgaaaag gctaagaaaa
      241 acaatattca tgagctggag tttaatgtta taaattctgt tgtttgcctg cttattgttt
      301 tcggcgtgtt tgcggcactg cttgtgatga agcgtgaaag cggttttata cagtcagaaa
      361 acagaaacct tgcgactttt ccgaaatttt cgctgtcatc atattttgac ggtgacttca
      421 caagcggaat aaccgaatat tttaccgaca ctgttccaaa cagagaaaag cttaaatctt
      481 tctgctctga tttcacgaat atgcttggaa taaagctcaa cgatactgtt ataactggcg
      541 atttcaaaac ggtggaaaaa gaagagctgg atcaggataa gatcgcaaaa acaactactg
      601 tgacggcttt tacaggttcg aggaccacca ccactgcgga tacaaagacc accgacacaa
      661 gcacaactac gaaaaaatca acggaaagcg aaaaggtagt tgacgtgcct gacaatcttg
      721 acgacggtca gtgggagggt gacgttatag tatccggcaa gggcgaaaac gtccgtgcag
      781 tgggtgcata ctatggcacg ttcgagaacg gcgacaagta tgcgaacacc ataaacaaat
      841 ggaaggctga ccttggggac agcgtgaacg tatacaatat gtctatcccg acttcggcgg
      901 cttattatat gccgaacaat ctcaaggacg ctgtatcaga tcagaaagac aatatcgaca
      961 acattgccgc aggtcttaac ggaataatca acactgatgt ttatgactct ctggctgagc
     1021 atacaaagga atatatctac tcaagaacgg atcatcactg gcagcctttg ggagcatatt
     1081 atgcggcgca ggtttttgcc gatcagtcgg gaatagattt tccagacctt gatacctatg
     1141 ataaatggga gatagacggc tttgtgggaa cgatgtatgc ttacagcaat tacaacagtg
     1201 agttgaaaaa atatcctgac aagttcattt actataagcc tgacaataat gatgatctga
     1261 cggtaaagta ctatgatacg gagtttaaaa accctgtgga gtctacgctg ttttttgact
     1321 atgcagaggg cgtgaactgc tattctgcga tactcaacgt ggatcaggag atagctgaga
     1381 tagatacggg cgtggacaat ggcagagtgc ttgtggtgtt caaagacagc tttggaaatg
     1441 ctctgatacc atttttcacc catggcttct caaaaatcta tgtttgtgat ttcagatatt
     1501 ttgatataaa cgctattgat ttttgtaagg aagtgggttg cacagacctg ctgtttgcaa
     1561 tatcgttgac gagctgttca acgcctagta aggtggattg tttgaataat attaggatac
     1621 agtaggagct aagtgaggag tggattaagt gaggagtgag gaatgaggaa tgggcaaagc
     1681 agaccaaagt aaattgcaag cataaagcaa aataaagcac cgagcgaagc gagagataaa
     1741 gcgcacgctc aagcccggcg ctagagggct tgtggggtct aggggcaaag cccctagcgg
     1801 agtttgaggc agagcctcat tcggcgcaag ccgacaaggt gcagatttaa tgaaaaat
//
LOCUS       DAWEMR010000286         1786 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_19078_length_1786_cov_11.604853,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000286 DAWEMR010000000
VERSION     DAWEMR010000286.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19078_length_1786_cov_11.604853"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1115..1124
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          435 a          460 c          316 g          565 t
ORIGIN      
        1 cctttaaaac tttgtggata atttttacag aacaaatcgg caggctcaga gcaaaatccg
       61 ctcaaagtct gccgttatgt tcgatatttt ttgttgtgta gggttcgact cccttttttc
      121 gttttctaaa catcaaaaaa gcccgcagaa gtctgcgagc tttttgtgtt gctactataa
      181 aatagatgac aataaaaatg ccaatttgac gtcatctatc aagcggtctt atcctaccga
      241 gtcagctgtc gttttatata ttccttcccc tcacaatcaa caaaatcctt ggcaaactgt
      301 tccgcctccg caaagctgtt ctccagtatc tcaaacccca cagccgcaga aaagctgtcg
      361 atctcaacac gttcggtctc gtctgcgaaa acctgtatta aaaatccttc gcaaagaaca
      421 tctctgccgt tcttgtcgac cctgctaatg tttttgtcgg gtgagatctt tatgtaaaat
      481 cctttgtatc tcatttgacc acgctcctta aaacttcatc tgaaaatcat tatcttcttc
      541 aaaatcctcg tccggttcat catcaaaatg ctgactgcaa accgaccttt caagtgccgc
      601 cctcagatag cttgtgtcca taccgttcgc ctcatatccc tgctttataa cattgtaata
      661 ataactggtt ggcggagaga tctgtccttt gttcatcaaa tacgccatgc actctttctt
      721 ttcgccgttg acttctatat caaacaactc cttgcggtaa agattcggga atccctcata
      781 cctatcaaga ctgtgttcgt cacgtccgtc tatctcccat atcagaacgg gtacttccga
      841 cttctcattc ggtacgatcg ttgccacacc acgaaattca agttcatagc cctgcatcat
      901 ttctgtgctt acaacctttg aattcggaca gcgatgcgcc atctgttcaa ggttgatgtt
      961 gctgccatag gcaatgtata atcttttgta ttccatgttt tcctccttac atactcattt
     1021 caaattcttc ttcaaattcg tcacattcac tttctgacgg ctcaatgtgt tcttcgggta
     1081 cattttcgac ttgcgtgctg ttgtcgctta caagnnnnnn nnnntttcag ctttgccctt
     1141 gcagcaattc catcctcagg gtgccgccag gcaatatcgc cctcaagatg tttcagaaga
     1201 tgtgtcctgc aattcttgaa ttcttcacct atcagtccaa gattcaaaag ccatacccta
     1261 aagctgtacc gcatattatc actctgcgaa acgtgaggag agcagtattt cttggtcatt
     1321 gccgcattgc ttatggctaa cgcaagcact atctgacttc ttacctctcc tgcgtgaaga
     1381 gaaccgttgt acgctcttat ctcataatgt ccgtgctgaa aaaagctgtg cagattgcag
     1441 atcacatatc ggctgtttga ataatgctga aactgctcgc tcattcttcc acgataccaa
     1501 agccttttta tctcctccat atctttgggc ttcttgcggt ttatgtcctc tatgaactgc
     1561 ttattcatct tgtgacagta gctttcacgg gcagatgata cctgcaaagc gtcccataga
     1621 aaatcttcct tgcttgcgaa gatgtttaca agatttcgta tctgctgcgg tgtataatcg
     1681 tctgccgaaa tgtgaatgtg cgtgcctgca caatatctcg gacctgtcac tccgcctgca
     1741 cggcgtaacg ctctgacaac ttcctgcaaa agcggaatat cttcat
//
LOCUS       DAWEMR010000287         1776 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_19210_length_1776_cov_10.314352,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000287 DAWEMR010000000
VERSION     DAWEMR010000287.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19210_length_1776_cov_10.314352"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          551 a          376 c          410 g          439 t
ORIGIN      
        1 gtgcaaagaa taaaaaaata ataatcagag caaaggctga cagctattcc ctttgcctgg
       61 cggtggacaa caccttcacc ggaactatcc tcaccgaccc cgaaggcggt ttcctttcct
      121 caaaacgcaa aggcaaaggg atcggcacag agtctgtcaa gaacattgcc gccaaataca
      181 atggcgtagc taaatttgaa tacggtgacg gaatgtttta cgcttccgta atgctgaaca
      241 tatgaaaaaa ggcggaaagc tcgcaaagag cctccgcttt ttctgtttta tcgtcctcca
      301 agctttacat tttcaaatgt tgccacaaca aataattcac atatcattta taaaatgttt
      361 cttgtatctt ttgtgcaaaa tgctataatc taattgaaaa tacggagaga attccgtata
      421 aagttaataa atcaaacaaa tcggaggtaa ttcattatga tgggaagact cagagcaatg
      481 cgtgagccgc tcaaatatgt tcagggcaga gatgctacac ttaagtttca tgaagaaatg
      541 ggctatatgg gcaaaaggtg gctgtttgta tgttcaaaca gtggctacaa agcctgtcat
      601 gacaagatag agaaaagctt tggcgagctt gacgattaca gacgctatga agtatttggt
      661 ggtatatcaa gcaagggcga gatagccaaa atggaagaga tagtaaaggc tgacaacatc
      721 gacacagtag ttgcagttgg cggcggaagc gcagttgata ctgcaaaagc tacagcatac
      781 tacacaggca agcacattgt tatcgtgcct acagttgctg caacagatgc gccatgtaca
      841 ggtctgtcag taatatacaa tgatgaccac agctttgaca aatatctttt ctatcctacc
      901 aatccagacg cagtaatggt agacacaaca gttatcgcaa acgcacctgt aaagttcctt
      961 attgcaggca tgggcgatgc acttggtaca tattttgaga gcagagcttc cataagaaca
     1021 gagtctgcaa gccttgaagg cacaggcatc acaagagcag gacaggcact tgcaagactt
     1081 tgctatgaca cgctgaaaac atacggcaag caggcagttg aggcttgcaa ggtacacgca
     1141 gttacacctg cccttgaagc tatctgcgag gctaacgtat atctttcagg tgttggagca
     1201 gataacgtaa actgtgcggc tgcacactcg ttctacaatg gcgtaacatc attgggaatc
     1261 aagcacgcag accacggctg ttgtgtagcc cttggtacac tggttcagct tgtacttgag
     1321 ggtgcgccaa aggaagagtt tgaagaggta cagaacttct gccttgaagt gggacttcct
     1381 gtcacactcg aggagatcgg tgtaactaca cctgagcagg tagaagctat cgcaaagaac
     1441 gcttgtatcc caggcgaaac tatccacaat cttgcaggcg acgtacagcc tatcgaactt
     1501 tatgacgcta tcatgcaggc agacgcaatg ggcaagatag cgctgggaaa ataaagaata
     1561 gtgtatattt aaaatgatat tgccacccac gactgatttg gctgtgggtg gttttttgtg
     1621 cgaaaaaaca gagaaacccg atcaatgggt ttctctgcat cttttagctg tattaaatca
     1681 caatatctta gtaaagtata tcttcctgcg aagctcagtt atctgcaacg tccataagct
     1741 caacagcaat atcgtcagac ccggtatcat ctgcgt
//
LOCUS       DAWEMR010000288         1694 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_20234_length_1694_cov_11.520439,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000288 DAWEMR010000000
VERSION     DAWEMR010000288.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20234_length_1694_cov_11.520439"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          495 a          389 c          319 g          491 t
ORIGIN      
        1 tgcatgaact tatcgagaag atagttgttt acgctcctga aaagagcagt gggcatcgta
       61 ctcagcagat agagatacac tttcgatttg atgtggctgt cgctacggca atagctgaca
      121 gcagagaata cgacaaaaag aaaaaggctg cgtaactgcg atgttacgca accttatctt
      181 caaatcacat aaactgcttt atgcgccccg accgaagtca gggctttttt tcatcagact
      241 gcttaaaccg tcacagcctg agtattattc tttttaagca cctcaataaa atctttttca
      301 ggaacaggct ttgagaagta gtatccctga atgaaatcac agcccatgcc aagaagcgtg
      361 tcgagctgat gcttgtcctc gataccctcg gcaactatct gcatattgag cttctttatc
      421 atcgccactg tactctcaat ggcgatatgc gccttttcgc tcttgaaaca ttcccagact
      481 atttcctttt caagcttgat aaacctgaaa ggcaggctga gaagatatgt catatttgaa
      541 tatcccgtac cataatcgtc aagtgcaaac ttcacaccat agtcggaaag ggtcttgatg
      601 tttttctcca taatgtgcgg agtatacacc gccgcagttt ccgttactcc aagacaaata
      661 gacgatgggt ctaccttgta tctgtccata atgctgagaa gctgctctac aaggtcagat
      721 tgcatacact ggactaccga taggtttacc tgcacatatt cgataccaag tgaagttccc
      781 acgcctttct ttataaatcg gcaggcttcc tcgaaaacat atttgcctat tttgagtatc
      841 ttgccgtttt cctcagctat aggaataaat tcctgtggag ggataaaacc aagcttttca
      901 tccttaagcc taacaagggc ttcacaggaa actatcttgt ttcttgaact tgaatatatc
      961 ggctgatagt atacctcaaa gccacgatta tccagagcct tgtctatagc attctctacc
     1021 tcgcttcgac gtttgctgtc ataaaagttt atgtcagttg catgaagaag tctttcggtt
     1081 tcaggttcac gatgcttgaa ctgctcgata aagtccacca gcatatctgc attgtcaacg
     1141 tcctcagggc agtcgatacg cataaagcag gctgttatca aagtatcaac agtacctata
     1201 ttccagtttt cagacagctt tttccttatt gcacgatacg tttcccccac cttttcagaa
     1261 tccttcggaa acataagagc aaagcattcg tcctccaaat aaaatccatc gcccagatta
     1321 acaaagttat aaagatagtc ggaaaagctt cttaacagag agttggaaaa acgtccgcca
     1381 aaggttttca taaacatttt gaaatccggt atccttatga gcaataatgt aaaatcagaa
     1441 gctgaggcat attttatgga acacctattg acaaagctgc tccagttgag cattttcgtt
     1501 ccttggtcta tcatatcgtc ctgactttgc aaagtgaaca ttattagaag catacataca
     1561 gatgccgcaa agcactccac aagaaggagt ttattgatca tctgaacaat taccgccact
     1621 acagtcattg agataaaggc tgttatagaa gtgaccacct gtcttgatag catacttcta
     1681 ttacccattg cgta
//
LOCUS       DAWEMR010000289         1672 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_20520_length_1672_cov_7.875077, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000289 DAWEMR010000000
VERSION     DAWEMR010000289.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20520_length_1672_cov_7.875077"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          410 a          394 c          327 g          541 t
ORIGIN      
        1 tatgttacag tagcggttac tccgttttcc gtcacattat cctccacttg tcggtcttta
       61 atctcatagt ctgagagaaa attttcctca tataaatacg ccttttcatg aacggcgtca
      121 agtgcctgct gttctgtcag ggtgtcttgt gaaaatttat attcttcaag ttcgcaggtg
      181 gttatgccaa aaggcatttc aagtccgaaa agcgacagag gagaatatct ctgctcattt
      241 ttccagtaac cctcaggctt tgagaaaaaa agagggatat cagcgtcaaa cacagtgaaa
      301 aaacgctttg tatctttctt gcctgtcgtc ttttctgcct tgttctcaaa gggctgtgtg
      361 aatgtgacag tgcgctcaaa agtgcctagt atcttaccct ttgagcgtgc atagcttgtc
      421 tttgtttcaa cgttttcctt gccgtctatc cagcttgatt ttgatgtgat gatctcacct
      481 cctacaacga tatcgccctt tgtcacaccg cttcccacag gtatctttag ctgtccgtca
      541 taaacctcga ttttttcgat aactccgttt tcgcaggcaa caagatttga gggatagcgg
      601 tttgactcac gctttgtgtc gccctcagtt tcgataacgt cgattatcag cgtgtcaccc
      661 ttgcgtgtta tgcctgccca tgatatgccc tccacttttt gctttaacgc acgttctgtg
      721 actatgagat caatgcttgg gatataagtg ccggtttcaa cgccctcctc tttaagcacc
      781 gacataacgc cttttctgat attttcatca tcactgagta tgtcaaacct caccaccacg
      841 tttgatagat agaagcatga aagcatgaca gccgcagtgc ctatgacaag tcctcctctt
      901 ccgaaaaaac gttttgcaag aacaaagctt ccgtgacgtt ttgtggcggt gtattcaagc
      961 ccaagttcat cgcatagtct ttcaagtgcc ttacagtgtt ccgcaggcat acgcaaagtg
     1021 agctttccgt tcttttcaca cattttgcta acgggaagct tttttgacat tatcctgcgg
     1081 aaaaacctgg tgaagctatc ccctgtgata acataatcta tataaccgaa aatcattatc
     1141 ctctctccct gatacttctt gacacaagct tgaccgactc tatttttcct cttatctcca
     1201 cgctgtcgga gcagaaattt gacattgtta gtccgctgcc ccatatctct atctcaaaag
     1261 agcctgtcag cacctttgcc attatctcac tgcactcaac tatctgcttg cagttttcga
     1321 taacggcggc tctgtcgtca gttatgctta tatatgagct tagtgcggtc aattcttttg
     1381 tcatgccgaa aaagcttaaa aatggtatct tcttcatttt tcgtcctccg ttcattgtct
     1441 tatgcttaat tatattatta gacagactga catatactta aaaagtaaaa gtagggaggt
     1501 aataaaaatg ttgaaaagac ttgcaaagga gctttgcgta tacatacttg cgggggtggt
     1561 catatggttc tgcattatca ccatatcagg gggcgagagc gtaaagcttg gtatctccga
     1621 agccgttgac cgctgtatga atatcataat cccgtcgctg tttgttttta tg
//
LOCUS       DAWEMR010000290         1544 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 NODE_22468_length_1544_cov_13.677636,
            whole genome shotgun sequence.
ACCESSION   DAWEMR010000290 DAWEMR010000000
VERSION     DAWEMR010000290.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22468_length_1544_cov_13.677636"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          475 a          311 c          349 g          409 t
ORIGIN      
        1 agtctatgat gagcgtaatt atagaatcat acgggctctt aataattttg ctgtagaaaa
       61 cgattacagg ttgtttgtat atcacacctg ctcagacctt tattggaata ctctgtctga
      121 aaaaggcgag cagtctgtgt ttgaccttat tgattataat ataacagatg ccgtcgtgac
      181 ctttgaagag ggtgtgtatg ctgagaatgt aatgacaaaa atcagaatgg acgccgccca
      241 atatggtaag cctgtcatat cagtcggcgt agagcatgac gattgcataa gcatcaaatt
      301 cgactatgca aaaggctttg agcaagtggt tcgccatgtg atagaacagc atggtgtaag
      361 agatataggc tttatcgcag gcagaaaaga cgaagaaaat tcagaagagc gtatagcagt
      421 tttcagaaag ctactcgagg agtatgatat cccattcaga aatgatgtct tttattacgg
      481 tgactattgg tctgttcctg ctttgaaagc agtggacgat atgatagaaa aaaataaagt
      541 cccaagagcg atcatctgtg caaacgatat gatggctata gccgcagggg ctgagtttca
      601 aagaaggggc tataagatcc cggaggatat tatcataaca ggctttgacg gctcagaaga
      661 agctaatttt tgcactccta tgcttacaac aagcggctgt agctatgatg ataccgctaa
      721 aacaatcatt aatgttataa gtaaagctct ggctggtgaa aagcccgaaa aaatatatac
      781 agtagactat gatctgtccg ttgaaaattc ctgcggctgt aaaccatcag tcgagcagat
      841 aaatacaggg gagtatatca gaatactccg agacagaatg aatcgctatc aggacgagga
      901 gcgtgtcctc tatgagcttg cagtgaatat catgaattgc gaaactccta agactcttgc
      961 cgccctgctg aaagagtatg cttttggcga tatggctgtt gtggtgaatg gtgatttctt
     1021 tgacgaacgt aaagaccctc gtgtaagcaa cagagaaccg ctttttgacg atgaaatgat
     1081 acttttgcgg agccgtgccg tggaagacag agatatccca cagccttttg accgcaccaa
     1141 catcattccc gatgtagaga atataatgga ctataaattc cccctcatat tctccgcact
     1201 tgcccatatc ggcatacccc taggctatgc ctgcttctat tttcctgtgc agctcaacga
     1261 gtattttaaa ataccactct atgtaaattc cctgaatacc gccttgggca gcttccgaag
     1321 cgtcacttat cagaagtata tgacaaagca tatcgaagaa atgtaccgtc atgatatgct
     1381 aacagggctt tataaccgca caggctttta taatgccctt gaaactctcc cacgaagaaa
     1441 caatcagctt gcattggtgg tttccgttga cgttgacgga cttaaataca taaacgataa
     1501 tttcggacac gaggcaggcg actatgcaat aagagccgcc gcaa
//
LOCUS       DAWEMR010000291         1441 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 k141_34920_length_1441_cov_14.0000, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000291 DAWEMR010000000
VERSION     DAWEMR010000291.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34920_length_1441_cov_14.0000"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          455 a          261 c          316 g          409 t
ORIGIN      
        1 aagaactgaa ctatgtcgta ttatacattt gtaaagttcg gctacattac agctaaaagc
       61 attttacttg acacctaaaa tcaatatctg acgaaaatgt ggctttgtgc atataataca
      121 aataacaaag tcacattttg ttgtataacg ccttgacttt gcgtcactta tactatatac
      181 ttgtattaag cactgttttt tatacaatat gttgtgattt gcggtgcgag ttgtgagaat
      241 aaaaaatata aggataaatc gggaaaatcg gaggcggtat tgtgataatc aagataaaaa
      301 agcgtgacgg acgtactgta actttcaata ttgagaaaat agctggcgct atatacaagg
      361 cggctcagtc tgtgggcaag gataactatg aggaagcttt ggagctttcg ggtaaagttg
      421 ttgataagct tatggaaaaa caccttgata tgcctactgt tgaagagatt caggattgcg
      481 ttgagcgtgt acttatcgag gagggcatgg ctgatactgc aaaaagttac atactctacc
      541 gctcaaacag aactcgtgca agagaaatga atacccgtct tatgaaagtt tatgaggatc
      601 ttactttcca atcagcgaaa gataacgatt tgaagcgtga gaatgctaat atagacggtg
      661 ataccgcaat gggtacaatg cttaaatacg gatctgtggg tgcaaaggag ttcaatgaga
      721 tgtatgttct tgctcctgag cattcaaagg ctcatcagga gggagatatc cacattcatg
      781 atcttgattt ttatacactg actaccactt gcactcagat agaccttaca aagctttttg
      841 ataagggttt ttctacaggt catggctttt tgcgtacacc aaatgatata caaagctatg
      901 cggcgcttgc ctgcattgcg atacaatcca atcagaacga ccagcatggt ggtcaatctc
      961 ttccgaagtt cgattatgat atggctgagg gtgtaagaaa gacattcaga caccgctatc
     1021 gtgacaatat cggcagggga cttgctctcc ttggtgaagt ttctgatgct cagagcattg
     1081 caaaaaaaat aactgaaatg cttgatgagc agggtttaaa gatcactctc gccaatgata
     1141 acggctatca agaggctgaa gcacagtttc ttgtgaattt tgttgacgca cctatagtga
     1201 agaagataca gagttttgca tataaaaact ctttgaaaga aactgacaga gccacatatc
     1261 aggcaatgga agcgcttatc cataatctta ataccatgaa ctcccgtgca ggcgctcaaa
     1321 cgcctttcag ctccataaat tatggcacgg atacttctat cgaaggcaga cttgttataa
     1381 agaatattct tcttgcagag gaagcaggac ttggcaatgg tgaaacgcct attttcccta
     1441 t
//
LOCUS       DAWEMR010000292         1425 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 k141_10673_length_1425_cov_12.0000, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000292 DAWEMR010000000
VERSION     DAWEMR010000292.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10673_length_1425_cov_12.0000"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          346 a          303 c          288 g          488 t
ORIGIN      
        1 gccgttaggc tcgttgcaga tctcgtaaat aacgttattg tagtccttgt aggtcttagc
       61 catttctgtg aagaacttga tagcttcatt tttgtggttg ttagggtttt ggtcattgag
      121 aacgtgccag tcgattatag catacattcc aagagacttt gcgttctcaa cgcctgtctt
      181 gactttctgc tttgcctgtg cctgatactt gccgttctcg gtgcagtagc caccccattc
      241 ttctgtgtac attgcaattc ttatagtgtt gaccttccag tcgtctctca gcactttaag
      301 agagtccttt gtaaggatat cggagaaatc ttcccacatg atgccgtgag ttgacatacc
      361 ctttatcttg aataccttgc cgttcttgtc aacaatattc gcaccattta cagaaagctg
      421 accatgctgt gaaactgggg tcacaacagc aggagcttta gatgtggtag ccgatacctc
      481 tgcagaatat tttccgtagt ggttggcttt cttgtcgtct gtcttgcagg ctcttatctt
      541 gaacttgtag gtggtcttct ggctgagatt ttttacagtg tagcttgtgg aagtggtctt
      601 tgcgatctct ttccactgac catttttaag ctgaaatact ctgtaatttg tacagcctat
      661 ctttgtccat gaaagcttta cgctgtcata agatacgctt gataccttta gaccctttgc
      721 aggctgtggg ttcgtggcgg tgttccagtt gtcggaatat gcaccgtaga ccttttttcc
      781 tttcacggtc ttgtaggctc ttaccttgaa gttttcacgg gtagcgtttg gcagattttt
      841 cacagtgtat gtgagggtcg agccactttt tacgtctgcg atctgcggat atttaccgtt
      901 ttttctcata caaactgagt atcccgaagc tccgctgacc tttttccagc taagctttat
      961 ctcgctgttg ctaagtgttt ttgaagtaag ccctgtgact tttccgacgc ttgccgcctc
     1021 aacagttatc gccgtctgcg aaagaaacgg cgtttcgtcc acagcaggca gtgccgccgc
     1081 agccgacatg agcgttaccg aagtcagcag cacagcagcg gctttcttca agctggttaa
     1141 ttttcccata atttttcctc caataataaa tattgtaact tttcattcat atttattata
     1201 agtttaacat attttcattc atttgtaaac agcaaaagcc tattttaaaa cttaaatact
     1261 tcacaaattt taaattcgtt cattggtgaa aatgtaataa atcctcccta tttactatag
     1321 aagtacgaaa aatgaataaa ttacaaatga ttaaaaaacg gtaacaagag cttttttgga
     1381 cagttgaaaa ctacagttga aaacttttga aaatgttgaa aacta
//
LOCUS       DAWEMR010000293         1359 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 k141_59290_length_1359_cov_13.0000, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000293 DAWEMR010000000
VERSION     DAWEMR010000293.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59290_length_1359_cov_13.0000"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          446 a          295 c          297 g          321 t
ORIGIN      
        1 tacaatacta atataagttt aaaaggagtg atgtaaaaat gagctatggt tttaagatca
       61 ttgtgacagg tgattacgct tgcttctcaa gacctgaaat gaaagttgag agggtgagtt
      121 acaacgtacc caccccaagc gcactggagg gacttatcaa atcagtatat tggaagcctg
      181 ccgtgcgcta tcagataaac aaaatagtgg tgttcaaccc tatcgagttt accaatatcc
      241 gacgaaacga ggtcaaagat aagctgctgt tatcatcagt gaaacagcaa atgaaaggcg
      301 gaggctcggc ggagatgtac acctcggaga tacgaagtca gcgtgcggca atggtgttga
      361 aaaatgtgaa atacggtatc gagttcacgt ttgagcgcac ctatcttaaa tcagaccacc
      421 cagacgaatc tgatgagaag cattataaca taatgctcag acgtctcaga aacggccagc
      481 atttcagaca gccatgcctt ggctgccgtg aatttgcaat aaagagtatt gaacttgttg
      541 acgactttga ccttacgcag atagcaccgc aaaatatggg tgataaagac cttggcttta
      601 tgctttacgg catgaagttt gaggacggag gcaagcctgt caataatgat tggaatgacc
      661 ctaagttttc tgacaaggcc gacgcagttt actaccgtcc gcatatgata gacggtgtta
      721 tcgatgttgc aaaatacagg gaggagatta aatgctgatt aaagccttgt gcgattacag
      781 tgctttgcag gagaataaaa ctcctgcagg ctattgtaag caagccatac actttgaaat
      841 tcttcttacc cccgagggca agatagccgc aataaacgat ttgagagtac cccgaattat
      901 ccctctgaaa aataaaaaga caaaaacagt ctatgatcca gtaatggaac agcttcccgt
      961 aagaacgcag aaaccgggca tagacaaaaa tacgatcgaa cacagaccac tctacatatt
     1021 cggtctgaac tatgataaag gaactttgac ccctacagac gctaccaaca aagcagtaaa
     1081 atcacacaaa tgcttttgcg aatggaatga gaagttcttt gagaatatcg actcgcctat
     1141 ttgtaaagcc tatcttaact ttatccgtac atggaaacct gagcaggaaa cccaaaaccc
     1201 acaccttctt gccctaggca aggagtattc aagctcctac tatgagtttg gacttgacgg
     1261 ctctcctgaa actaaacttc acaatgacag caaattcaaa gccgcctatg aggattacct
     1321 cgcccagctt aacgccgaca gcgacagtga cgataccga
//
LOCUS       DAWEMR010000294         1081 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 k141_22601_length_1081_cov_10.0000, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000294 DAWEMR010000000
VERSION     DAWEMR010000294.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22601_length_1081_cov_10.0000"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          346 a          188 c          261 g          286 t
ORIGIN      
        1 taattgaatg agaatgttat cagagtttat tttaaggagt tcattaagaa gaaaaagcgg
       61 tgagaatttt gaagaaagca gttacactta tagatatagc caaagcctgt gacacaagta
      121 atgtcactgt gtcaaaagcc cttgcaggaa aaagcggagt tagcgatgct ctcagagaga
      181 agataaagca ggtcgctgac gaaatgggtt atgtgggcgt gaaaagcact cagaacaggg
      241 gaggaaatac tatcggtgtg cttatcccat caaaattcat caatcctaac ggttcatttt
      301 attgggcatt gtataacgct cttgtaacaa ggctcaaaaa agagaatatg ttctgcataa
      361 tggaaaattt agatcaggag gacgaggaca ggctcatgct cccgcattgt atcgaggaca
      421 agcaggtttc agcacttatt tcactgggac agctttcaaa gaattatgca aagcttctta
      481 atgacaatgt gaaagatctt gtactgcttg attattatgt gccgtcctta gacttggacg
      541 ctgttgtgac aaatggctac agcggaggct acaagcttac ttcatatctt ataaaaatgg
      601 gtcacaagaa gataggatac ataggaagta aatttgccac aacgagtatt tttgacagat
      661 atatgggcta tgtaaaggct atgatagagc atggctatga ggtcaacgaa aagtggcgta
      721 tcgacgatcg ttacaagcgt gattttatca ctatgacttt ccctgaggac gatatgccaa
      781 cggctttcgt atgcaactgc gacgaggcgg cttaccgtgc aataagacag ctccgtggca
      841 tgggatataa cgtgcctgag gatatttcca tcgtgggata tgataactat ttgatctccg
      901 aagtttcaga cccgactatc acgactataa acgttgacgc agattatatg gcagaccttg
      961 cggtaatgac acttatggat agaatggaag aacctgacgg caagccaagg ataataacta
     1021 tcgagggcga tctcgttata aaggactctg tgaagagaat atagtgcgtt tttattagca
     1081 a
//
LOCUS       DAWEMR010000295         1010 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus bicirculans (ex Wegman et al. 2014)
            isolate gSpreadC_ID_3547 k141_52285_length_1010_cov_10.0000, whole
            genome shotgun sequence.
ACCESSION   DAWEMR010000295 DAWEMR010000000
VERSION     DAWEMR010000295.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37997059
            Sequence Read Archive: SRR8800843
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bicirculans (ex Wegman et al. 2014) (human gut
            metagenome)
  ORGANISM  Ruminococcus bicirculans (ex Wegman et al. 2014)
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Ruminococcus bicirculans (ex Wegman et al.
                     2014)"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52285_length_1010_cov_10.0000"
                     /isolate="gSpreadC_ID_3547"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1160721"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          344 a          181 c          215 g          270 t
ORIGIN      
        1 atgatcttat tttcatagac gagtttcagg acgtgaataa tcttcaggag cttgttttcc
       61 gctcgctttc tgattctgat aacttggata tcatgggcaa aaatgttttt gtggttggcg
      121 atataaagca ggctatatat cgtttcagac tctcaaatcc tgaacttttc agcaaaacga
      181 gaaatgacgc ttccgcacct gaaaacagcg aacagctcga ggctatcttg ctaagcaaga
      241 atttcagaag tcgtgagtct gtggttgatt ttgtaaattt tctgttccac tatcttatga
      301 cacagcacac aggtcaggtg gattatgata gaaatgaaga acttcgatac ggtgaacttt
      361 atgataaaaa taacaagcct acagaaataa tgctcatacc ttttgataag gactacaaaa
      421 agacaaatgg ctattctgtg gagaacaaag taatagcaca aaagataaag cacatgcttg
      481 aaaacggtga ggaagtcaaa gatcatggct ctctaagacc gtgccgtgca ggagattttt
      541 gtatacttgt tcaaaagaat gatgaaatta cccttatggc aaaagccctt gaagaagtag
      601 gtctgaaagc atattctgag gatacaaacg gatacatgaa gtcaagagaa ataacacttg
      661 tgctgaatat gctcagggta attgacaatc ctatgaacga tattgcaatg acagctatca
      721 tgatgtcgcc tatcatggga tttacgcctg atgaaatggc ggcagtaaca gaaaagtcaa
      781 agaagcctaa cagcaaactg aaagaccata tctatcaggt ggtagcggcg tcagcttctg
      841 ctgaggacag ccacgaaaaa gaagctactc atataagctt taataataaa acattgcagg
      901 ataagtgcat aaagcttaat aagcttatta attccctgcg ttatctttca atgagcatgg
      961 gtcttgaacg gctcataaga aaaatatacg acatgaccga ccttatgggg
//
